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Sample records for globigerinoides ruber pink

  1. Boron/calcium ratios in Globigerinoides ruber from the Arabian Sea: Implications for controls on boron incorporation

    Digital Repository Service at National Institute of Oceanography (India)

    Naik, S.S.; Naidu, P.D.

    of Globigerinoides ruber from the Ocean Drilling Program (ODP) Site 723A (western Arabian Sea) and Core AAS9/21 (eastern Arabian Sea) from the last 22 kyr were analysed for B/Ca, delta18O and Mg/Ca. B/Ca was compared to reconstructed salinity and sea...

  2. The Effects of Temperature and Salinity on Mg Incorporation in Planktonic Foraminifera Globigerinoides ruber (white): Results from a Global Sediment Trap Mg/Ca Database

    Science.gov (United States)

    Gray, W. R.; Weldeab, S.; Lea, D. W.

    2015-12-01

    Mg/Ca in Globigerinoides ruber is arguably the most important proxy for sea surface temperature (SST) in tropical and sub tropical regions, and as such guides our understanding of past climatic change in these regions. However, the sensitivity of Mg/Ca to salinity is debated; while analysis of foraminifera grown in cultures generally indicates a sensitivity of 3 - 6% per salinity unit, core-top studies have suggested a much higher sensitivity of between 15 - 27% per salinity unit, bringing the utility of Mg/Ca as a SST proxy into dispute. Sediment traps circumvent the issues of dissolution and post-depositional calcite precipitation that hamper core-top calibration studies, whilst allowing the analysis of foraminifera that have calcified under natural conditions within a well constrained period of time. We collated previously published sediment trap/plankton tow G. ruber (white) Mg/Ca data, and generated new Mg/Ca data from a sediment trap located in the highly-saline tropical North Atlantic, close to West Africa. Calcification temperature and salinity were calculated for the time interval represented by each trap/tow sample using World Ocean Atlas 2013 data. The resulting dataset comprises >240 Mg/Ca measurements (in the size fraction 150 - 350 µm), that span a temperature range of 18 - 28 °C and 33.6 - 36.7 PSU. Multiple regression of the dataset reveals a temperature sensitivity of 7 ± 0.4% per °C (p < 2.2*10-16) and a salinity sensitivity of 4 ± 1% per salinity unit (p = 2*10-5). Application of this calibration has significant implications for both the magnitude and timing of glacial-interglacial temperature changes when variations in salinity are accounted for.

  3. A comparison of Mg/Ca ratios in Globigerinoides ruber (white): sensu stricto versus a mixture of genotypes

    Digital Repository Service at National Institute of Oceanography (India)

    Naik, S.S.

    at the University of Southampton, UK, using G. ruber, sensu stricto only. Cleaned samples were dissolved in 100-300 µl 0.5M HNO3. Mg/Ca ratios were analysed on a Thermo Finnigan Element 2 ICP-MS. Long term precision for Mg/Ca is 3% (2σ) based on repeat.... Acknowledgements: I thank Jimin Yu of LDEO and Miguel Martinez-Boti of the University of Southampton for the analytical help. This work was funded by the INDOUSSTF fellowship and a DST Fast Track Project to SN. This is National Institute of Oceanography...

  4. The effects of temperature, salinity, and the carbonate system on Mg/Ca in Globigerinoides ruber (white): A global sediment trap calibration

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    Gray, William R.; Weldeab, Syee; Lea, David W.; Rosenthal, Yair; Gruber, Nicolas; Donner, Barbara; Fischer, Gerhard

    2018-01-01

    The Mg/Ca of planktic foraminifera Globigerinoides ruber (white) is a widely applied proxy for tropical and sub-tropical sea-surface temperature. The accuracy with which temperature can be reconstructed depends on how accurately relationships between Mg/Ca and temperature and the multiple secondary controls on Mg/Ca are known; however, these relationships remain poorly quantified under oceanic conditions. Here, we present new calibrations based on 440 sediment trap/plankton tow samples from the Atlantic, Pacific and Indian Oceans, including 130 new samples from the Bay of Bengal/Arabian Sea and the tropical Atlantic Ocean. Our results indicate temperature, salinity and the carbonate system all significantly influence Mg/Ca in G. ruber (white). We propose two calibration models: The first model assumes pH is the controlling carbonate system parameter. In this model, Mg/Ca has a temperature sensitivity of 6.0 ± 0.8%/°C (2σ), a salinity sensitivity of 3.3 ± 2.2%/PSU and a pH sensitivity of - 8.3 ± 7.7%/0.1 pH units; The second model assumes carbonate ion concentration ([3 2-CO]) is the controlling carbonate system parameter. In this model, Mg/Ca has a temperature sensitivity of 6.7 ± 0.8%/°C, a salinity sensitivity of 5.0 ± 3.0%/PSU and a [3 2-CO] sensitivity of - 0.24 ± 0.11%/μmol kg-1. In both models, the temperature sensitivity is significantly lower than the widely-applied sensitivity of 9.0 ± 0.6%/°C. Application of our new calibrations to down-core data from the Last Glacial Maximum, considering whole ocean changes in salinity and carbonate chemistry, indicate a cooling of 2.4 ± 1.6°C in the tropical oceans if pH is the controlling parameter and 1.5 ± 1.4°C if [3 2-CO] is the controlling parameter.

  5. Fossil and Genetic Evidence for the Polyphyletic Nature of the Planktonic Foraminifera "Globigerinoides", and Description of the New Genus Trilobatus

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    Spezzaferri, Silvia; Kucera, Michal; Pearson, Paul Nicholas; Wade, Bridget Susan; Rappo, Sacha; Poole, Christopher Robert; Morard, Raphaël; Stalder, Claudio

    2015-01-01

    Planktonic foraminifera are one of the most abundant and diverse protists in the oceans. Their utility as paleo proxies requires rigorous taxonomy and comparison with living and genetically related counterparts. We merge genetic and fossil evidence of “Globigerinoides”, characterized by supplementary apertures on spiral side, in a new approach to trace their “total evidence phylogeny” since their first appearance in the latest Paleogene. Combined fossil and molecular genetic data indicate that this genus, as traditionally understood, is polyphyletic. Both datasets indicate the existence of two distinct lineages that evolved independently. One group includes “Globigerinoides” trilobus and its descendants, the extant “Globigerinoides” sacculifer, Orbulina universa and Sphaeroidinella dehiscens. The second group includes the Globigerinoides ruber clade with the extant G. conglobatus and G. elongatus and ancestors. In molecular phylogenies, the trilobus group is not the sister taxon of the ruber group. The ruber group clusters consistently together with the modern Globoturborotalita rubescens as a sister taxon. The re-analysis of the fossil record indicates that the first “Globigerinoides” in the late Oligocene are ancestral to the trilobus group, whereas the ruber group first appeared at the base of the Miocene with representatives distinct from the trilobus group. Therefore, polyphyly of the genus "Globigerinoides" as currently defined can only be avoided either by broadening the genus concept to include G. rubescens and a large number of fossil species without supplementary apertures, or if the trilobus group is assigned to a separate genus. Since the former is not feasible due to the lack of a clear diagnosis for such a broad genus, we erect a new genus Trilobatus for the trilobus group (type species Globigerina triloba Reuss) and amend Globoturborotalita and Globigerinoides to clarify morphology and wall textures of these genera. In the new

  6. Nucleus Ruber of Actinopterygians.

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    Nakayama, Tomoya; Miyajima, Satoshi; Nishino, Hirotaka; Narita, Junya; Abe, Hideki; Yamamoto, Naoyuki

    2016-01-01

    Nucleus ruber is known as an important supraspinal center that controls forelimb movements in tetrapods, and the rubral homologue may serve similar functions in fishes (motor control of pectoral fin). However, two apparently different structures have been identified as 'nucleus ruber' in actinopterygians. One is nucleus ruber of Goldstein (1905) (NRg), and the other nucleus ruber of Nieuwenhuys and Pouwels (1983) (NRnp). It remains unclear whether one of these nuclei (or perhaps both) is homologous to tetrapod nucleus ruber. To resolve this issue from a phylogenetic point of view, we have investigated the distribution of tegmental neurons retrogradely labeled from the spinal cord in eight actinopterygian species. We also investigated the presence/absence of the two nuclei with Nissl- or Bodian-stained brain section series of an additional 28 actinopterygian species by comparing the morphological features of candidate rubral neurons with those of neurons revealed by the tracer studies. Based on these analyses, the NRg was identified in all actinopterygians investigated in the present study, while the NRnp appears to be absent in basal actinopterygians. The phylogenetic distribution pattern indicates that the NRg is the more likely homologue of nucleus ruber, and the NRnp may be a derived nucleus that emerged during the course of actinopterygian evolution. © 2016 S. Karger AG, Basel.

  7. Microbial Ecophysiology of Vibrio ruber

    Directory of Open Access Journals (Sweden)

    Tjaša Danevčič

    2014-01-01

    Full Text Available Bacteria use different adaptation strategies to survive environmental perturbations. In this minireview, adaptation strategies of new red-pigmented Vibrio ruber isolated from coastal environments to different environmental stresses (i.e. salinity, viscosity, UV light, mitomycin C, nutrient availability and temperature are reviewed. To cope with environmental stresses Vibrio ruber uses several different adaptive strategies. For example, lipid composition as well as phase behaviour are strongly dependent on salt concentration. Vibrio ruber membrane has no hydroxy fatty acids, but exceptionally high lysolipid content compared to other related Vibrio species. Inorganic nutrient uptake by bacteria is selective, depends on environmental conditions and varies several fold with environmental perturbations. Protein composition, carbon flow through the central metabolic pathways, energy generation as well as secondary metabolite production adapt readily to stress conditions. The activity of glucose-6-phosphate dehydrogenase proved to be a good indicator of Vibrio ruber stress. Cells are able to modulate their local viscosity in response to variations of environmental viscosity. The bacterium harbours several viral genetic elements in its genome, which could be induced by mitomycin C. Environmental conditions during growth of bacteria have a significant effect on lysate carbon turnover. Secondary metabolite prodigiosin confers protection against UV in the environment, which adds to the known repertoire of prodigiosin ecophysiological functions. In conclusion, Vibrio ruber in its short acquaintance with the scientific community (less than ten years has proven to be an immensely valuable model system for ecophysiological studies of bacteria.

  8. A comparison of Globigerinoides ruber calcification between upwelling and non-upwelling regions in the Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Naik, S.S.; Godad, S.P.; Naidu, P.D.; Ramaswamy, V.

    stream_size 24687 stream_content_type text/plain stream_name J_Earth_Syst_Sci_122_1153.pdf.txt stream_source_info J_Earth_Syst_Sci_122_1153.pdf.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 A... Keywords. Arabian Sea; pCO2; shell weights; flux; SST. J. Earth Syst. Sci. 122, No. 4, August 2013, pp. 1153–1159 c© Indian Academy of Sciences 1153 1154 Sushant S Naik et al. calcification and further what could be the fate of planktonic foraminifera under...

  9. Pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria.

    Science.gov (United States)

    Gilardi, G L; Faur, Y C

    1984-10-01

    Twenty-one strains of pink-pigmented bacteria, isolated from human clinical specimens and an environmental source, were compared with Pseudomonas mesophilica ATCC 29983 and Protaminobacter ruber ATCC 8457. These isolates were gram-negative, oxidative rods which were motile by means of a single polar flagellum; gave positive catalase, indophenol oxidase, urease, and amylase reactions; and grew slowly at 30 degrees C. Fourteen isolates conformed to the designated type strains Pseudomonas mesophilica ATCC 29983 and Protaminobacter ruber ATCC 8457. The remaining seven strains represented an undescribed taxon. These pink bacteria appear to be invaders of debilitated patients with an underlying chronic disease.

  10. VISCOSITY DICTATES METABOLIC ACTIVITY of Vibrio ruber

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    Maja eBoric

    2012-07-01

    Full Text Available Little is known about metabolic activity of bacteria, when viscosity of their environment changes. In this work, bacterial metabolic activity in media with viscosity ranging from 0.8 to 29.4 mPas was studied. Viscosities up to 2.4 mPas did not affect metabolic activity of Vibrio ruber. On the other hand, at 29.4 mPas respiration rate and total dehydrogenase activity increased 8 and 4-fold, respectively. The activity of glucose-6-phosphate dehydrogenase increased up to 13-fold at higher viscosities. However, intensified metabolic activity did not result in faster growth rate. Increased viscosity delayed the onset as well as the duration of biosynthesis of prodigiosin. As an adaptation to viscous environment V. ruber increased metabolic flux through the pentose phosphate pathway and reduced synthesis of a secondary metabolite. In addition, V. ruber was able to modify the viscosity of its environment.

  11. Chemical Profile of Monascus ruber Strains

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    Ahamed M. Moharram

    2012-01-01

    Full Text Available Chemical profile of Monascus ruber strains has been studied using gas chromatography-mass spectrometry (GC/MS analysis. The colour intensity of the red pigment and secondary metabolic products of two M. ruber strains (AUMC 4066 and AUMC 5705 cultivated on ten different media were also studied. Metabolic products can be classified into four categories: anticholesterol, anticancer, food colouring, and essential fatty acids necessary for human health. Using GC/MS, the following 88 metabolic products were detected: butyric acid and its derivatives (25 products, other fatty acids and their derivatives (19 products, pyran and its derivatives (22 products and other metabolites (22 products. Among these, 32 metabolites were specific for AUMC 4066 strain and 34 for AUMC 5705 strain, whereas 22 metabolites were produced by both strains on different tested substrates. Production of some metabolites depended on the substrate used. High number of metabolites was recorded in the red pigment extract obtained by both strains grown on malt broth and malt agar. Also, 42 aroma compounds were recorded (4 alcohols, 2 benzaldehydes, 27 esters, 3 lactones, 1 phenol, 1 terpenoid, 3 thiol compounds and acetate-3-mercapto butyric acid. Thin layer chromatography and GC/MS analyses revealed no mycotoxin citrinin in any media used for the growth of the two M. ruber strains.

  12. Pseudomonas mesophilica and an unnamed taxon, clinical isolates of pink-pigmented oxidative bacteria.

    OpenAIRE

    Gilardi, G L; Faur, Y C

    1984-01-01

    Twenty-one strains of pink-pigmented bacteria, isolated from human clinical specimens and an environmental source, were compared with Pseudomonas mesophilica ATCC 29983 and Protaminobacter ruber ATCC 8457. These isolates were gram-negative, oxidative rods which were motile by means of a single polar flagellum; gave positive catalase, indophenol oxidase, urease, and amylase reactions; and grew slowly at 30 degrees C. Fourteen isolates conformed to the designated type strains Pseudomonas mesoph...

  13. [Lichen ruber planus : Better understanding, better treatment!

    Science.gov (United States)

    Schilling, L; Vogt, T

    2018-02-01

    Lichen ruber, also called lichen ruber planus or lichen planus (LP), is a noncontagious inflammatory skin disease. LP is the main representative and namesake of the group of lichenoid diseases, which are characterized by small papules often accompanied by severe itching. With 65% of cases, LP is primarily a disease of the mucous membranes. In 20% of the cases, the disease is found on the skin and mucous membranes; skin involvement alone is seen in only about 10% of cases. Cutaneous LP has a very favorable 1‑year prognosis of almost 80% healing as opposed to the mucosa and the adnexal organs. Histologically, keratinocytes with vacuolar degeneration, leaving behind apoptotic Kamino bodies and the characteristic band-shaped lymphocytic infiltrate at the dermatoepithelial junction, are common to lichenoid diseases. The horny layer is firm and compact and the stratum granulosum is thickened as a correlate of the Wickham stripes. The molecular pathogenesis, still partially hypothetical, assumes trigger factors leading to the presentation of intrinsic or foreign antigens. The triggered inflammation becomes independent in the sense of a classical cell-mediated autoimmune disease. Other autoimmune diseases are often associated with LP. Classical anti-inflammatory-immunosuppressive therapeutic concepts dominate with systemic retinoids ranking first in the highest evidence class for cutaneous LP with limitations in treatment of both mucosal and adnexal LP. More recently, interesting and new complementary phototherapeutics have been identified.

  14. Pink urine syndrome

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    Luis del Carpio-Orantes

    2017-03-01

    Full Text Available In the present images we allude to a syndrome of low incidence, characterized by pink urine, being related to factors such as obesity, and being triggered by abdominal surgeries, use of propofol, among others. Being favoured by the presence of abundant crystals of uric acid in the urine confers the typical pink coloration.

  15. Pink Eye (Conjunctivitis)

    Science.gov (United States)

    ... or child care if you're not able to take time off — just stay consistent in practicing good hygiene. Preventing pink eye in newborns Newborns' eyes are susceptible to bacteria normally present in the mother's birth canal. ...

  16. Pink Eye: What To Do

    Centers for Disease Control (CDC) Podcasts

    In this podcast, CDC's Adam Cohen, MD, a pediatrician and parent, discusses conjunctivitis (pink eye), a common eye condition in children and adults. He reviews pink eye causes and treatment, suggestions on when to call or visit a doctor, and practical tips to prevent pink eye from spreading.

  17. Pink-line syndrome

    Digital Repository Service at National Institute of Oceanography (India)

    Ravindran J.; Raghukumar, C.; Manikandan, B.

    , a physiological crisis in the scleractinian coral Porites lutea. Marine biology 149: 347-356 7. Ravindran J, Raghukumar C (2006a) Histological observations on the scleractinian coral Porites lutea affected by pink-line syndrome. Current science... handbook: guidelines for assessment and monitoring. Currie Communications, Melbourne, Australia, 94p+App 10. Vargas-Angel B, Wheeler B (2009) Coral health and disease assessment in the US Pacific territories and affiliated states. Proc. 11th Coral Reef...

  18. Pink Eye: What To Do

    Centers for Disease Control (CDC) Podcasts

    2010-10-12

    In this podcast, CDC's Adam Cohen, MD, a pediatrician and parent, discusses conjunctivitis (pink eye), a common eye condition in children and adults. He reviews pink eye causes and treatment, suggestions on when to call or visit a doctor, and practical tips to prevent pink eye from spreading.  Created: 10/12/2010 by National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 10/13/2010.

  19. PINK1-Interacting Proteins: Proteomic Analysis of Overexpressed PINK1

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    Aleksandar Rakovic

    2011-01-01

    Full Text Available Recent publications suggest that the Parkinson's disease- (PD- related PINK1/Parkin pathway promotes elimination of dysfunctional mitochondria by autophagy. We used tandem affinity purification (TAP, SDS-PAGE, and mass spectrometry as a first step towards identification of possible substrates for PINK1. The cellular abundance of selected identified interactors was investigated by Western blotting. Furthermore, one candidate gene was sequenced in 46 patients with atypical PD. In addition to two known binding partners (HSP90, CDC37, 12 proteins were identified using the TAP assay; four of which are mitochondrially localized (GRP75, HSP60, LRPPRC, and TUFM. Western blot analysis showed no differences in cellular abundance of these proteins comparing PINK1 mutant and control fibroblasts. When sequencing LRPPRC, four exonic synonymous changes and 20 polymorphisms in noncoding regions were detected. Our study provides a list of putative PINK1 binding partners, confirming previously described interactions, but also introducing novel mitochondrial proteins as potential components of the PINK1/Parkin mitophagy pathway.

  20. IDENTITY OF THE PINK-PIGMENTED METHANOL-OXIDIZING BACTERIA AS VIBRIO EXTORQUENS.

    Science.gov (United States)

    STOCKS, P K; MCCLESKEY, C S

    1964-10-01

    Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological, cultural, and physiological characteristics. All the strains utilized methanol, formate, oxalate, succinate, glycerol, and benzene as sole carbon sources; methanol, formate, and glycerol afforded best growth. Most strains utilized fructose and ribose; other carbohydrates tested were not available as carbon and energy sources. There was strain variation in the use of hexane, heptane, n-propanol, n-butanol, acetate, and propionate. Methane, ethane, n-propane, and n-butane were not utilized. Our isolates, and Pseudomonas methanica of Harrington and Kallio (not the methane-dependent P. methanica of Dworkin and Foster), Pseudomonas AM1 of Peele and Quayle, Pseudomonas PRL-W4 of Kaneda and Roxburgh, and Protaminobacter ruber den Dooren de Jong are nearly identical with Vibrio extorquens (Bassalik) Bhat and Barker, and should be considered the same species.

  1. Investigation of Pink Tourmalines by X-ray Fluorescent Technique

    International Nuclear Information System (INIS)

    Sangariyavanich, A.; Na Songkhla, S.; Pimjum, S.

    1998-01-01

    X-ray fluorescent technique has been employed in the study of trace elements in six samples of gamma irradiated pink tourmalines, namely, red-pink (rubellite), light-pink, orange-pink, brownish orange-pink, purple red and purple orange-pink. The analysis of their characteristic X-ray indicated the existence of manganese in all samples. Trace amounts of iron, zinc, lead, bismuth or gallium were also investigated in certain samples. Since these elements were not present in red-pink tourmaline, therefore, we believed that manganese is the major cause of pink color in tourmaline while other elements produce various types of pink color

  2. Re-evaluation of the holotype of Mus ruber Jentink, 1880 (Rodentia: Muridae) from western New Guinea (Irian Jaya)

    NARCIS (Netherlands)

    Calaby, J.H.; Mary Taylor, J.

    1980-01-01

    INTRODUCTION The first rodent from the New Guinea region, now included in the genus Rattus, to be formally named, was Mus ruber Jentink, 1880. The name R. ruber is currently in widespread use (Lidicker, 1968, 1973; Lidicker & Ziegler, 1968; Misonne, 1969; Ziegler, 1971; Bulmer & Menzies, 1972, 1973;

  3. Molecular characterization of three 3-ketosteroid-Delta(1)-dehydrogenase isoenzynnes of Rhodococcus ruber strain Chol-4

    NARCIS (Netherlands)

    Fernandez de las Heras, Laura; van der Geize, Robert; Drzyzga, Oliver; Perera, Julian; Navarro Llorens, Juana Maria

    2012-01-01

    Rhodococcus ruber strain Chol-4 isolated from a sewage sludge sample is able to grow on minimal medium supplemented with steroids, showing a broad catabolic capacity. This paper reports the characterization of three different 3-ketosteroid-Delta(1)-dehydrogenases (KstDs) in the genome of R. ruber

  4. The acyl-CoA binding protein affects Monascus pigment production in Monascus ruber CICC41233.

    Science.gov (United States)

    Long, Chuannan; Liu, Mengmeng; Chen, Xia; Wang, Xiaofang; Ai, Mingqiang; Cui, Jingjing; Zeng, Bin

    2018-02-01

    The present study verified whether acyl-coenzyme A (acyl-CoA)-binding protein (ACBP) affected the production of Monascus pigments (MPs) in Monascus ruber CICC41233 (MrACBP). Phylogenetic analysis revealed that the cloned Mracbp gene, which encoded the MrACBP protein, exhibited the closest match (99% confidence level) to the gene from Penicilliopsis zonata . The MrACBP and maltose-binding protein (MBP) were simultaneously expressed in Escherichia coli Rosetta DE3 in the form of a fusion protein. The microscale thermophoresis binding assay revealed that the purified MBP-MrACBP exhibited a higher affinity for myristoyl-CoA (Kd = 88.16 nM) than for palmitoyl-CoA (Kd = 136.07 nM) and octanoyl-CoA (Kd = 270.9 nM). Further, the Mracbp gene was homologously overexpressed in M. ruber CICC41233, and a positive transformant M. ruber ACBP5 was isolated. The fatty acid myristic acid in M. ruber ACBP5 was lower than that in the parent strain M. ruber CICC41233. However, when compared with the parent strain, the production of total MPs, water-soluble pigment, and ethanol-soluble pigment in M. ruber ACBP5 increased by 11.67, 9.80, and 12.70%, respectively, after 6 days. The relative gene expression level, as determined by a quantitative real-time polymerase chain reaction analysis, of the key genes acbp , pks , mppr1 , fasA , and fasB increased by 4.03-, 3.58-, 1.67-, 2.11-, and 2.62-fold after 6 days. These data demonstrate the binding preference of MrACBP for myristoyl-CoA, and its influence on MPs production.

  5. Salinibater Ruber as a Model for the Habitability of Europa's Ocean

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    Cardona, M. C.; Ramírez, S. I.

    2017-11-01

    The moon Europa has an ocean enriched with sulfate compounds. This work evaluates the adaptation strategies of Salinibacter ruber, a halophilic bacterium, when subjected to MgSO4 and NaSO4, two of the main salty components of Europás ocean.

  6. Observations on the behaviour of the Scarlet Ibis, Eudocimus ruber, in Artis Zoo, Amsterdam

    NARCIS (Netherlands)

    Spil, R.E.; Walstijn, van M.W.; Albrecht, H.

    1985-01-01

    The Scarlet Ibis, Eudocimus ruber (Linnaeus), fam. Threskiornithidae, is the closest relative of the White Ibis, Eudocimus albus (Linnaeus). The two species live in adjoining geographical areas. Nothing is known about the Scarlet Ibis’s breeding biology in the wild and only little about its breeding

  7. Autoradiographic study of the nucleolar RNA metabolism during the oogenesis of Lineus ruber Mueller (Heteronemertes)

    International Nuclear Information System (INIS)

    Rue, Gerard; Gontcharoff, Marie

    1975-01-01

    During Lineus ruber oogenesis, there is a very high uridine uptake by the oocyte before the yolk formation. At the end of this stage, the nucleolus shows a special structure that seems to be related to a decrease of the ribosomal RNA synthesis. During the yolk formation, the nucleolus scatters in the nucleus, allowing ribonucleoprotein granules to go towards the cytoplasm [fr

  8. Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. ruber

    Czech Academy of Sciences Publication Activity Database

    Baldwin, H. J.; Vallo, Peter; Gardner, M. G.; Drosten, C.; Tschapka, M.; Stow, A. J.

    2014-01-01

    Roč. 7, č. 607 (2014), s. 607 ISSN 1756-0500 Institutional support: RVO:68081766 Keywords : Bat * Hipposideridae * Hipposideros caffer * Hipposideros ruber * Microchiroptera * Microsatellites * Population genetics Subject RIV: EG - Zoology

  9. Influence of Light Intensity on Growth and Pigment Production by Monascus ruber in Submerged Fermentation.

    Science.gov (United States)

    Bühler, Rose Marie Meinicke; Müller, Bruna Luíse; Moritz, Denise Esteves; Vendruscolo, Francielo; de Oliveira, Debora; Ninow, Jorge Luiz

    2015-07-01

    To reduce environmental problems caused by glycerine accumulation and to make the production of biodiesel more profitable, crude glycerin without treatment was used as substrate for obtaining higher value-added bioproducts. Monascus ruber is a filamentous fungus that produces pigments, particularly red ones, which are used for coloring foods (rice wine and meat products). The interest in developing pigments from natural sources is increasing due to the restriction of using synthetic dyes. The effects of temperature, pH, microorganism morphology, aeration, nitrogen source, and substrates have been studied in the cultivation of M. ruber. In this work, it was observed that light intensity is also an important factor that should be considered for understanding the metabolism of the fungus. In M. ruber cultivation, inhibition of growth and pigment production was observed in Petri dishes and blaffed flasks exposed to direct illumination. Growth and pigment production were higher in Petri dishes and flasks exposed to red light and in the absence of light. Radial growth rate of M. ruber in plates in darkness was 1.50 mm day(-1) and in plates exposed to direct illumination was 0.59 mm day(-1). Maximum production of red pigments (8.32 UA) and biomass (8.82 g L(-1)) were obtained in baffled flasks covered with red film and 7.17 UA of red pigments, and 7.40 g L(-1) of biomass was obtained in flasks incubated in darkness. Under conditions of 1248 lux of luminance, the maximum pigment production was 4.48 UA, with production of 6.94 g L(-1) of biomass, indicating that the fungus has photoreceptors which influence the physiological responses.

  10. Physiological role of vitamin B12 in a methanol-utilizing bacterium, Protaminobacter ruber

    International Nuclear Information System (INIS)

    Shimizu, S.; Ueda, S.; Sato, K.

    1984-01-01

    The methanol-utilizing bacterium Protaminobacter ruber is able to produce a relatively large amount of vitamin B 12 . The present study aims at the physiological role of vitamin B 12 in P. ruber. P. ruber was found to contain the two sequential reactions of glutamate mutase with β-methylaspartase and propionyl-CoA carboxylase with methylmalonyl-CoA mutase. Considering the presence of these enzyme systems and the reaction from mesaconyl-CoA to glyoxylate and propionyl-CoA, it could be considered that the formation of glutamate from α-ketoglutarate, the conversion of glutamate to mesaconate via β-methylaspartate, the activation of mesaconate with CoA to form mesaconyl-CoA, the cleavage of mesaconyl-CoA to glyoxylate and propionyl-CoA, the carboxylation of propionyl-CoA to methylmalonyl-CoA, and the isomerization of methylmalonyl-CoA to succinyl-CoA require cobalamine as a cofactor. 29 refs., 2 figs., 2 tabs

  11. Health and pink-collar work.

    Science.gov (United States)

    Basu, S; Ratcliffe, G; Green, M

    2015-10-01

    In recent years, there has been a decline in the manufacturing sector of the UK economy with a corresponding growth of service-orientated pink-collar jobs in some regions. While the health outcomes of white- and blue-collar workers are well-established, less is known about this emerging pink-collar group. To outline the health of pink-collar workers in comparison to their white-collar counterparts across a range of indicators. Area-level percentages for white-, pink- and blue-collar workers were derived from residents' routinely collected employment data in a northern English town. Area-level health data pertaining to male and female life expectancy, respiratory deaths and deaths from cardiovascular and circulatory causes (all age and under 75 years) were obtained from the local authority and public health observatory. Multivariate regression analyses were performed to assess relationships between job collar and health. When adjusted for deprivation, areas with higher percentages of pink-collar workers experienced lower rates of death from circulatory disease under the age of 75 in comparison to white-collar workers. Other relationships between collar status and health outcomes were not statistically significant. The reasons underlying the apparent protective effect of pink-collar status for deaths from circulatory disease are uncertain and merit further study. Possibilities include differences in age, exposure to occupational hazards and lifestyle behaviours. Our work has a number of limitations and longitudinal studies with detailed exposure data should assess the long-term health outcomes of these workers using agreed definitions. © The Author 2015. Published by Oxford University Press on behalf of the Society of Occupational Medicine. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Chum and pink salmon genetics - Genetic and life history variation of southern chum and pink salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The distribution of genetic and life history variation in chum (Oncorhynchus keta) and pink (O. gorbuscha) salmon in their southern range in North America is key to...

  13. Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910.

    Science.gov (United States)

    Huang, Tao; Tan, Hailing; Lu, Fangju; Chen, Gong; Wu, Zhenqiang

    2017-11-21

    Monascus pigments are widely used in the food and pharmaceutical industries due to their safety to human health. Our previous study found that glucose concentration induced extracellular oxidoreduction potential (ORP) changes could influence extracellular water-soluble yellow pigment production by Monascus ruber CGMCC 10910 in submerged fermentation. In this study, H 2 O 2 and dithiothreitol (DTT) were used to change the oxidoreduction potential for investigating the effects of oxidative or reductive substances on Monascus yellow pigment production by Monascus ruber CGMCC 10910. The extracellular ORP could be controlled by H 2 O 2 and DTT. Both cell growth and extracellular water-soluble yellow pigment production were enhanced under H 2 O 2 -induced oxidative (HIO) conditions and were inhibited under dithiothreitol-induced reductive conditions. By optimizing the amount of H 2 O 2 added and the timing of the addition, the yield of extracellular water-soluble yellow pigments significantly increased and reached a maximum of 209 AU, when 10 mM H 2 O 2 was added on the 3rd day of fermentation with M. ruber CGMCC 10910. Under HIO conditions, the ratio of NADH/NAD+ was much lower than that in the control group, and the expression levels of relative pigment biosynthesis genes were up-regulated; moreover, the activity of glucose-6-phosphate dehydrogenase (G6PDH) was increased while 6-phosphofructokinase (PFK) activity was inhibited. Oxidative conditions induced by H 2 O 2 increased water-soluble yellow pigment accumulation via up-regulation of the expression levels of relative genes and by increasing the precursors of pigment biosynthesis through redirection of metabolic flux. In contrast, reductive conditions induced by dithiothreitol inhibited yellow pigment accumulation. This experiment provides a potential strategy for improving the production of Monascus yellow pigments.

  14. Seeing the World Through "Pink-Colored Glasses": The Link Between Optimism and Pink.

    Science.gov (United States)

    Kalay-Shahin, Lior; Cohen, Allon; Lemberg, Rachel; Harary, Gil; Lobel, Thalma E

    2016-12-01

    This study investigated optimism, which is considered a personality trait, from the grounded cognition perspective. Three experiments were conducted to investigate the association between pink and optimism. In Experiment 1A, 22 undergraduates (10 females; M age  = 23.68) were asked to classify words as optimistic or pessimistic as fast as possible. Half the words were presented in pink and half in black. Experiment 1B (N = 24; 14 females; M age  = 22.82) was identical to 1A except for the color of the words-black and light blue instead of pink-to rule out the possible influence of brightness. Experiment 2 exposed 144 participants (74 females; M age  = 25.18) to pink or yellow and then measured their optimism level. The findings for Experiments 1A and 1B indicated an association between pink and optimism regardless of brightness. Experiment 2 found that mere exposure to pink increased optimism levels for females. These results contribute to the dynamic view of personality, current views on optimism, and the growing literature on grounded cognition. © 2015 Wiley Periodicals, Inc.

  15. Genome Analysis of Fimbriiglobus ruber SP5T, a Planctomycete with Confirmed Chitinolytic Capability.

    Science.gov (United States)

    Ravin, Nikolai V; Rakitin, Andrey L; Ivanova, Anastasia A; Beletsky, Alexey V; Kulichevskaya, Irina S; Mardanov, Andrey V; Dedysh, Svetlana N

    2018-04-01

    Members of the bacterial order Planctomycetales have often been observed in associations with Crustacea. The ability to degrade chitin, however, has never been reported for any of the cultured planctomycetes although utilization of N -acetylglucosamine (GlcNAc) as a sole carbon and nitrogen source is well recognized for these bacteria. Here, we demonstrate the chitinolytic capability of a member of the family Gemmataceae , Fimbriiglobus ruber SP5 T , which was isolated from a peat bog. As revealed by metatranscriptomic analysis of chitin-amended peat, the pool of 16S rRNA reads from F. ruber increased in response to chitin availability. Strain SP5 T displayed only weak growth on amorphous chitin as a sole source of carbon but grew well with chitin as a source of nitrogen. The genome of F. ruber SP5 T is 12.364 Mb in size and is the largest among all currently determined planctomycete genomes. It encodes several enzymes putatively involved in chitin degradation, including two chitinases affiliated with the glycoside hydrolase (GH) family GH18, GH20 family β- N -acetylglucosaminidase, and the complete set of enzymes required for utilization of GlcNAc. The gene encoding one of the predicted chitinases was expressed in Escherichia coli , and the endochitinase activity of the recombinant enzyme was confirmed. The genome also contains genes required for the assembly of type IV pili, which may be used to adhere to chitin and possibly other biopolymers. The ability to use chitin as a source of nitrogen is of special importance for planctomycetes that inhabit N-depleted ombrotrophic wetlands. IMPORTANCE Planctomycetes represent an important part of the microbial community in Sphagnum -dominated peatlands, but their potential functions in these ecosystems remain poorly understood. This study reports the presence of chitinolytic potential in one of the recently described peat-inhabiting members of the family Gemmataceae , Fimbriiglobus ruber SP5 T This planctomycete uses

  16. Sugarcane bagasse hydrolysate as a potential feedstock for red pigment production by Monascus ruber.

    Science.gov (United States)

    Terán Hilares, Ruly; de Souza, Rebeca Andrade; Marcelino, Paulo Franco; da Silva, Silvio Silvério; Dragone, Giuliano; Mussatto, Solange I; Santos, Júlio César

    2018-04-15

    Sugarcane bagasse (SCB) hydrolysate could be an interesting source for red pigment production by Monascus ruber Tieghem IOC 2225. The influence of different wavelength of light-emitting diode (LED) at 250 μmol.m -2 .s -1 of photon flux density on red pigment production by M. ruber in glucose-based medium was evaluated. Then, SCB hydrolysate was used as carbon source under the previously selected light incidence conditions. In glucose-based medium, the highest pigment production was achieved in fermentation assisted with orange LED light (8.28 UA 490nm ), white light (8.26 UA 490nm ) and under dark condition (7.45 UA 490nm ). By using SCB hydrolysate-based medium, the highest red pigment production (18.71 AU 490nm ) was achieved under dark condition and the glucose and cellobiose present in the hydrolysate were metabolized. SCB enzymatic hydrolysate was demonstrated to be a promising carbon source for high thermal stability red pigment production (activation energy of 10.5 kcal.mol -1 ), turning an interesting alternative for implementation in biorefineries. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Production of tannase from Aspergillus ruber under solid-state fermentation using jamun (Syzygium cumini) leaves.

    Science.gov (United States)

    Kumar, Rakesh; Sharma, Jitender; Singh, Randhir

    2007-01-01

    Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzyphus mauritiana), jamun leaves (Syzygium cumini), amla leaves (Phyllanthus emblica) and jawar leaves (Sorghum vulgaris). Jamun leaves were found to be the best substrate for enzyme production under solid-state fermentation (SSF). In SSF with jamun leaves, the maximum production of tannase was found to be at 30 degrees C after 96 h of incubation. Tap water was found to be the best moistening agent, with pH 5.5 in ratio of 1:2 (w/v) with substrate. Addition of carbon and nitrogen sources to the medium did not increase tannase production. Under optimum conditions as standardized here, the enzyme production was 69 U/g dry substrate. This is the first report on production of tannase by A. ruber, giving higher yield under SSF with agro-waste as the substrate.

  18. The rise and fall of pink disease.

    Science.gov (United States)

    Dally, A

    1997-08-01

    This paper explores the social and medical history and context of pink disease (acrodynia), a serious disease of infants and young children that baffled the medical world during the first half of the twentieth century until it was shown to be caused by mercury poisoning. In the English-speaking world the commonest source of the mercury was teething powders, which were widely available and advertised with increasing sophistication. Efforts to control them (such as the BMJ's campaign against 'Secret Remedies') were as yet unsuccessful. The article discusses the social conditions that influenced the existence and recognition of pink disease, the delay in finding its cause, the way in which it was explained as a virus infection or nutritional deficiency and why it seldom occurred outside the teething period. It discusses both professional and lay attitudes to health and diseases during the early twentieth century and provides a model of how the disease developed in a specific social setting and how the medical profession attempted to deal with it within the limitations of contemporary professional thought. The resistance to the evidence of mercury poisoning is typical of resistance to new medical knowledge and declined only when the opponents and sceptics grew old and disappeared from the scene. Meanwhile, the cause having been identified and accepted, pink disease disappeared, but its consequences emerged much later, in an unexpected quarter, as a cause of male infertility.

  19. Flight activity of Noack's round-leafbat (Hipposideros cf. ruber) at two caves in central Ghana, West Africa

    Czech Academy of Sciences Publication Activity Database

    Nkrumah, E. E.; Badu, E. K.; Baldwin, H. J.; Anti, P.; Klose, S. M.; Vallo, Peter; Drosten, C.; Kalko, E. K. V.; Oppong, S. K.; Tschapka, M.

    2017-01-01

    Roč. 19, č. 2 (2017), s. 347-355 ISSN 1508-1109 Institutional support: RVO:68081766 Keywords : prey * insects * temperature * caves * flight activity * Hipposideros cf. ruber Subject RIV: EG - Zoology OBOR OECD: Zoology Impact factor: 1.040, year: 2016

  20. Advertisement call of Scinax camposseabrai (Bokermann, 1968) (Anura: Hylidae), with comments on the call of three species of the Scinax ruber clade.

    Science.gov (United States)

    Novaes, Gabriel; Zina, Juliana

    2016-02-25

    Scinax camposseabrai was allocated into the Scinax ruber clade by Caramaschi & Cardoso (2006) by overall similarities as snout not pointed, breeding in open areas, and an advertisement calls with multipulsed notes. This assumption about the call was based solely on an onomatopoeia provided by Bokermann (1968). Herein we provide a formal description of the advertisement call of S. camposseabrai and compare it with described calls of other S. ruber clade species. Additionally, we provide descriptions of the advertisement calls of three sympatric species of the S. ruber clade: S. eurydice (Bokermann), S. pachycrus (Miranda-Ribeiro) and S. cf. x-signatus.

  1. Effect of amino acids on red pigments and citrinin production in Monascus ruber.

    Science.gov (United States)

    Hajjaj, Hassan; François, Jean-Marie; Goma, Gérard; Blanc, Philippe J

    2012-03-01

    Amino acids were used as sole nitrogen sources to examine their effects on the production of water-soluble red pigments and citrinin by Monascus ruber ATCC 96218 cultivated on chemically defined media. In general, when glycine, tyrosine, arginine, serine, or histidine were used as sole nitrogen sources, they favored the production of red pigments, and restricted the synthesis of the mycotoxin. In contrast, the production of citrinin was enhanced in media supplemented with either glutamate, alanine, or proline. Histidine was found to be the most valuable amino acid as it resulted in the highest production of red pigments and almost completely eliminated the formation of mycotoxin. © 2012 Institute of Food Technologists®

  2. Optimal C:N ratio for the production of red pigments by Monascus ruber.

    Science.gov (United States)

    Said, Farhan M; Brooks, John; Chisti, Yusuf

    2014-09-01

    The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10-15) compared with the C:N ratio in the red pigments (e.g. >20) of the fungus Monascus ruber. Therefore, determining an optimal C:N ratio in the culture medium for maximizing the production of the pigments is important. A culture medium composition is established for maximizing the production of the red pigment by the fungus M. ruber ICMP 15220 in submerged culture. The highest volumetric productivity of the red pigment was 0.023 AU L(-1) h(-1) in a batch culture (30 °C, initial pH of 6.5) with a defined medium of the following composition (g L(-1)): glucose (10), monosodium glutamate (MSG) (10), MgSO4·7H2O (0.5), KH2PO4 (5), K2HPO4 (5), ZnSO4·7H2O (0.01), FeSO4·7H2O (0.01), CaCl2 (0.1), MnSO4·H2O (0.03). This medium formulation had a C:N mole ratio of 9:1. Under these conditions, the specific growth rate of the fungus was 0.043 h(-1) and the peak biomass concentration was 6.7 g L(-1) in a 7-day culture. The biomass specific productivity of the red pigment was 1.06 AU g(-1) h(-1). The best nitrogen source proved to be MSG although four other inorganic nitrogen sources were evaluated.

  3. Fimbriiglobus ruber gen. nov., sp. nov., a Gemmata-like planctomycete from Sphagnum peat bog and the proposal of Gemmataceae fam. nov.

    Science.gov (United States)

    Kulichevskaya, Irina S; Ivanova, Anastasia A; Baulina, Olga I; Rijpstra, W Irene C; Sinninghe Damsté, Jaap S; Dedysh, Svetlana N

    2017-02-01

    An aerobic, budding, dark pink to red-pigmented bacterium was isolated from an acidic boreal Sphagnum peat bog and designated strain SP5T. Cells of this strain were non-motile spheres that were uniformly covered with crateriform pits and fimbria, and tended to form aggregates during growth in liquid media. Strain SP5T was capable of growth between pH 4.0 and pH 6.8 (optimum at pH 5.5-6.0) and at temperatures between 10 and 30 °C (optimum at 20-25 °C). The preferred growth substrates were sugars and some heteropolysaccharides. The major fatty acids were C20 : 1ω9c, C16 : 1ω9c and C16 : 0, and the major polar lipid was trimethylornithine. Cells contained also significant amounts of bound (ω-1)OH-C30 : 1 fatty acid. The quinone was menaquinone-6, and the G+C content of the DNA was 60.7 mol%. Strain SP5T was a member of the order Planctomycetales and belonged to the phylogenetic lineage defined by the genus Gemmata. It displayed 88 and 89 % 16S rRNA gene sequence similarity to Gemmata obscuriglobusUQM 2246T and 'Gemmata massiliana' IIL30, 89 % to Zavarzinella formosa A10T and 86 % to Telmatocola sphagniphila SP2T. However, strain SP5T differed from members of these genera by cell morphology, substrate utilization pattern and fatty acid composition. Based on these data, the novel isolate should be considered as representing a novel species of a new genus of planctomycetes, for which the name Fimbriiglobus ruber gen. nov., sp. nov, is proposed. The type strain is SP5T (=LMG 29572T=VKM B-3045T). We also suggest the establishment of a novel family, Gemmataceaefam. nov., which includes the phylogenetically related genera Gemmata, Zavarzinella, Telmatocola and Fimbriiglobus.

  4. Various Stages of Pink Fungus (Upasia salmonicolor in Java

    Directory of Open Access Journals (Sweden)

    Ambarwati Harsojo Tjokrosoedarmo

    1995-12-01

    Full Text Available Pink fungus in Java is classified as Upasia salmonicolor (Basidiomycetes: Corticiaceae and its anamorph is Necator decretus. This fungus is a serious pathogen which attacks many woody plants. The pink fungus in Java exhibits five developmental stages on the surface of the host bark: I. An initial cobweb stage as thin, white, cobweb-like hyphal layer, which creeps over the surface of the bark, during which penetration of the host occurs; II. Pseudonodular stage, as conical white pustules occurring only on lenticels or cracks, and only on shady side of branches; III. Teleomorph, occurs as pink incrustation and pink pustules on shady side of branches; IV. Nodular stages, as globose white pustules occurring chiefly on intact bark, but also on the lenticels or cracks, on exposed side of branches; V. Anamorph, as small orange-red sporodochium, on exposed side of branches. Key words: pink fungus, Corticiaceae, Basidiomycetes, Necator

  5. Effects of Light Intensity and Color on the Biomass, Extracellular Red Pigment, and Citrinin Production of Monascus ruber.

    Science.gov (United States)

    Wang, Liling; Dai, Yang; Chen, Wanping; Shao, Yanchun; Chen, Fusheng

    2016-12-21

    Light is a crucial environmental signal for fungi. In this work, the effects of different light intensities and colors on biomass, Monascus pigments (MPs) and citrinin production of Monascus ruber M7 were investigated. We have demonstrated that low intensity of blue light (500 lx) decreased Monascus biomass, increased MPs accumulation via upregulation of MpigA, MpigB, and MpigJ genes expression, but had no significant influence on citrinin production. High intensity of blue light (1500 lx) decreased citrinin accumulation but had no significant influence on biomass and MPs production after 14 days cultivation. Low intensity of green light (500 lx) stimulated citrinin production via upregulation of pksCT, mrl1, mrl2, and ctnA genes expression. One putative red light photoreceptor and two putative green light photoreceptors were identified in M. ruber M7. These observations will not only guide the practical production of Monascus but also contribute to our understanding light effects on Monascus.

  6. Production of water-soluble yellow pigments via high glucose stress fermentation of Monascus ruber CGMCC 10910.

    Science.gov (United States)

    Wang, Meihua; Huang, Tao; Chen, Gong; Wu, Zhenqiang

    2017-04-01

    Monascus pigments are secondary metabolites of Monascus species and are mainly composed of yellow pigments, orange pigments and red pigments. In this study, a larger proportion of Monascus yellow pigments could be obtained through the selection of the carbon source. Hydrophilic yellow pigments can be largely produced extracellularly by Monascus ruber CGMCC 10910 under conditions of high glucose fermentation with low oxidoreduction potential (ORP). However, keeping high glucose levels later in the culture causes translation or a reduction of yellow pigment. We presume that the mechanism behind this phenomenon may be attributed to the redox level of the culture broth and the high glucose stress reaction of M. ruber CGMCC 10910 during high glucose fermentation. These yellow pigments were produced via high glucose bio-fermentation without citrinin. Therefore, these pigments can act as natural pigments for applications as food additives.

  7. Properties of Thermus ruber Strains Isolated from Icelandic Hot Springs and DNA:DNA Homology of Thermus ruber and Thermus aquaticus

    Science.gov (United States)

    Sharp, Richard J.; Williams, Ralph A. D.

    1988-01-01

    Seventeen pink-pigmented strains of the genus Thermus were isolated from samples collected from thermal areas of Iceland. The strains were examined by using phenotypic characterization and DNA:DNA homology and were compared with recognized strains. Visually, the strains could be divided into three groups based on their pigmentation; however, spectroscopic studies of the pigments indicated little difference among them. Most strains required a vitamin supplement for growth and used fructose, maltose, mannose, or sucrose as the sole carbon source. In the presence of nitrate, two strains were able to grow under anaerobic conditions. The optimum growth temperature was 60°C; growth did not occur at 30 or 70°C. PMID:16347714

  8. Using millet as substrate for efficient production of monacolin K by solid-state fermentation of Monascus ruber.

    Science.gov (United States)

    Zhang, Bo-Bo; Xing, Hong-Bo; Jiang, Bing-Jie; Chen, Lei; Xu, Gan-Rong; Jiang, Yun; Zhang, Da-Yong

    2018-03-01

    In this study, various grains such as rice, millet, corn, barley and wheat were used as raw materials for monacolin K production by solid-state fermentation of Monascus ruber. Among these substrates, millet was found to be the best one for monacolin K production, by which the yield reached 7.12 mg/g. For enhanced monacolin K production, the effects of fermentation time, charge amount, initial moisture content and inoculum volume were systematically investigated in the solid-state fermentation of M. ruber. Moreover, complementary carbon source and nitrogen source were added for further improving the production of monacolin K. Results showed that the maximum production of monacolin K (19.81 mg/g) could be obtained at the optimal conditions. Compared with the traditional red mold rice, using millet as substrate is promising for high production of monacolin K in the solid-state fermentation of M. ruber. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  9. Functional characterization of 3-ketosteroid 9α-hydroxylases in Rhodococcus ruber strain chol-4.

    Science.gov (United States)

    Guevara, Govinda; Heras, Laura Fernández de Las; Perera, Julián; Llorens, Juana María Navarro

    2017-09-01

    The 3-Ketosteroid-9α-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9α-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with a 3-ketosteroid-Δ 1 -dehydrogenase activity (KstD), being both responsible of the steroid nucleus (rings A/B) breakage. KshAB initiates the opening of the steroid ring by the 9α-hydroxylation of the C9 carbon of 4-ene-3-oxosteroids (e.g. AD) or 1,4-diene-3-oxosteroids (e.g. ADD), transforming them into 9α-hydroxy-4-androsten-3,17-dione (9OHAD) or 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD), respectively. The redundancy of these enzymes in the actinobacterial genomes results in a serious difficulty for metabolic engineering this catabolic pathway to obtain intermediates of industrial interest. In this work, we have identified three homologous kshA genes and one kshB gen in different genomic regions of R. ruber strain Chol-4. We present a set of data that helps to understand their specific roles in this strain, including: i) description of the KshAB enzymes ii) construction and characterization of ΔkshB and single, double and triple ΔkshA mutants in R. ruber iii) growth studies of the above strains on different substrates and iv) genetic complementation and biotransformation assays with those strains. Our results show that KshA2 isoform is needed for the degradation of steroid substrates with short side chain, while KshA3 works on those molecules with longer side chains. KshA1 is a more versatile enzyme related to the cholic acid catabolism, although it also collaborates with KshA2 or KshA3 activities in the catabolism of steroids. Accordingly to what it is described for other Rhodococcus strains, our results also suggest that the side chain degradation is KshAB-independent. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Pink shrimp as an indicator for restoration of everglades ecosystems

    Science.gov (United States)

    Browder, Joan A.; Robblee, M.B.

    2009-01-01

    The pink shrimp, Farfantepenaeus duorarum, familiar to most Floridians as either food or bait shrimp, is ubiquitous in South Florida coastal and offshore waters and is proposed as an indicator for assessing restoration of South Florida's southern estuaries: Florida Bay, Biscayne Bay, and the mangrove estuaries of the lower southwest coast. Relationships between pink shrimp and salinity have been determined in both field and laboratory studies. Salinity is directly relevant to restoration because the salinity regimes of South Florida estuaries, critical nursery habitat for the pink shrimp, will be altered by changes in the quantity, timing, and distribution of freshwater inflow planned as part of the Comprehensive Everglades Restoration Project (CERP). Here we suggest performance measures based on pink shrimp density (number per square meter) in the estuaries and propose a restoration assessment and scoring scheme using these performance measures that can readily be communicated to managers, policy makers, and the interested public. The pink shrimp is an appropriate restoration indicator because of its ecological as well as its economic importance and also because scientific interest in pink shrimp in South Florida has produced a wealth of information about the species and relatively long time series of data on both juveniles in estuarine nursery habitats and adults on the fishing grounds. We suggest research needs for improving the pink shrimp performance measure.

  11. Molecular characterization of three 3-ketosteroid-Δ(1)-dehydrogenase isoenzymes of Rhodococcus ruber strain Chol-4.

    Science.gov (United States)

    Fernández de las Heras, Laura; van der Geize, Robert; Drzyzga, Oliver; Perera, Julián; María Navarro Llorens, Juana

    2012-11-01

    Rhodococcus ruber strain Chol-4 isolated from a sewage sludge sample is able to grow on minimal medium supplemented with steroids, showing a broad catabolic capacity. This paper reports the characterization of three different 3-ketosteroid-Δ(1)-dehydrogenases (KstDs) in the genome of R. ruber strain Chol-4. The genome of this strain does not contain any homologues of a 3-keto-5α-steroid-Δ(4)-dehydrogenase (Kst4d or TesI) that appears in the genomes of Rhodococcus erythropolis SQ1 or Comamonas testosteroni. Growth experiments with kstD2 mutants, either a kstD2 single mutant, kstD2 double mutants in combination with kstD1 or kstD3, or the triple kstD1,2,3 mutant, proved that KstD2 is involved in the transformation of 4-androstene-3,17-dione (AD) to 1,4-androstadiene-3,17-dione (ADD) and in the conversion of 9α-hydroxy-4-androstene-3,17-dione (9OHAD) to 9α-hydroxy-1,4-androstadiene-3,17-dione (9OHADD). kstD2,3 and kstD1,2,3 R. ruber mutants (both lacking KstD2 and KstD3) did not grow in minimal medium with cholesterol as the only carbon source, thus demonstrating the involvement of KstD2 and KstD3 in cholesterol degradation. In contrast, mutation of kstD1 does not alter the bacterial growth on the steroids tested in this study and therefore, the role of this protein still remains unclear. The absence of a functional KstD2 in R. ruber mutants provoked in all cases an accumulation of 9OHAD, as a branch product probably formed by the action of a 3-ketosteroid-9α-hydroxylase (KshAB) on the AD molecule. Therefore, KstD2 is a key enzyme in the AD catabolism pathway of R. ruber strain Chol-4 while KstD3 is involved in cholesterol catabolism. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Aerobic Biodegradation of N-Nitrosodimethylamine by the Propanotroph Rhodococcus ruber ENV425▿

    Science.gov (United States)

    Fournier, Diane; Hawari, Jalal; Halasz, Annamaria; Streger, Sheryl H.; McClay, Kevin R.; Masuda, Hisako; Hatzinger, Paul B.

    2009-01-01

    The propanotroph Rhodococcus ruber ENV425 was observed to rapidly biodegrade N-nitrosodimethylamine (NDMA) after growth on propane, tryptic soy broth, or glucose. The key degradation intermediates were methylamine, nitric oxide, nitrite, nitrate, and formate. Small quantities of formaldehyde and dimethylamine were also detected. A denitrosation reaction, initiated by hydrogen atom abstraction from one of the two methyl groups, is hypothesized to result in the formation of n-methylformaldimine and nitric oxide, the former of which decomposes in water to methylamine and formaldehyde and the latter of which is then oxidized further to nitrite and then nitrate. Although the strain mineralized more than 60% of the carbon in [14C]NDMA to 14CO2, growth of strain ENV425 on NDMA as a sole carbon and energy source could not be confirmed. The bacterium was capable of utilizing NDMA, as well as the degradation intermediates methylamine and nitrate, as sources of nitrogen during growth on propane. In addition, ENV425 reduced environmentally relevant microgram/liter concentrations of NDMA to <2 ng/liter in batch cultures, suggesting that the bacterium may have applications for groundwater remediation. PMID:19542346

  13. Behavioural laterality as a predictor of health in captive Caribbean flamingos (Phoenicopterus ruber): an exploratory analysis.

    Science.gov (United States)

    Anderson, Matthew J; Ialeggio, Donna M

    2014-01-01

    The present study sought to explore the possibility that lateral behaviour in captive Caribbean flamingos (Phoenicopterus ruber) housed at the Philadelphia Zoo (Philadelphia, PA) could be used to predict a variety of physiological measures of health obtained via complete blood counts (CBC) and plasma biochemistry analyses that were performed as part of the flock's annual physical examination. Consistent with previous research, evidence of rightward lateral neck-resting preferences were obtained, no evidence was found for the existence of leg stance preferences, and neck-resting and leg stance preferences were shown to be unrelated. Both lateral neck-resting preferences and lateral support leg preference were shown to be related to a variety of measures from the CBC and plasma biochemistry analyses. While several general trends emerged in regards to the CBC variables, the relationships between the lateral behaviours and those variables generated via plasma biochemistry analyses proved to be fewer and somewhat less consistent. Birds with rightward neck-resting preferences and birds with leftward support leg preferences generally appeared to be healthier and less stressed according to the CBC measures; however, the validity of lateral leg stance preference as a predictor of health and wellbeing is questionable given the lack of statistically significant leg stance preferences.

  14. Production of red pigments by Monascus ruber in culture media containing corn steep liquor

    Directory of Open Access Journals (Sweden)

    P. S. Hamano

    2006-12-01

    Full Text Available The production of red pigments by Monascus ruber was evaluated utilizing complex culture media composed of glucose or sucrose (10 g/L, corn steep liquor (5 or 10 g/L and monosodium glutamate (0, 5.0, 7.6, 11.4 or 15.2 g/L. Medium containing 10 g/L glucose, 5 g/L corn steep liquor and 7.6 g/L monosodium glutamate resulted the highest values of extracellular red pigment absorbance (20.7 U and productivity (0.35 U/h. This medium also produced better results than using semi-synthetic medium with analytical grade reagents (12.4 U and 0.21 U/h. The cell growth was similar in both media (X @ 6.5 g/L, indicating that the capacity of the cells to produce red pigments was higher in complex culture media. In addition, in the complex culture medium, less of the intracellular red pigments accumulated than in semi-synthetic medium (9.1% and 30%, respectively.

  15. Kinetic of orange pigment production from Monascus ruber on submerged fermentation.

    Science.gov (United States)

    Vendruscolo, Francielo; Schmidell, Willibaldo; de Oliveira, Débora; Ninow, Jorge Luiz

    2017-01-01

    Pigments produced by species of Monascus have been used to coloring rice, meat, sauces, wines and beers in East Asian countries. Monascus can produce orange (precursor), yellow and red pigments. Orange pigments have low solubility in culture media and when react with amino groups they become red and largely soluble. The orange pigments are an alternative to industrial pigment production because the low solubility facilitates the downstream operations. The aim of this work was to study the kinetic on the production of orange pigments by Monascus ruber CCT 3802. The shaking frequency of 300 rpm was favorable to production, whereas higher shaking frequencies showed negative effect. Pigment production was partially associated with cell growth, the critical dissolved oxygen concentration was between 0.894 and 1.388 mgO 2  L -1 at 30 °C, and limiting conditions of dissolved oxygen decreased the production of orange pigments. The maintenance coefficient (mo) and the conversion factor of oxygen in biomass (Yo) were 18.603 mgO 2  g x -1  h -1 and 3.133 g x  gO 2 -1 and the consideration of these parameters in the oxygen balance to estimate the biomass concentration provided good fits to the experimental data.

  16. Monascus ruber as cell factory for lactic acid production at low pH.

    Science.gov (United States)

    Weusthuis, Ruud A; Mars, Astrid E; Springer, Jan; Wolbert, Emil Jh; van der Wal, Hetty; de Vrije, Truus G; Levisson, Mark; Leprince, Audrey; Houweling-Tan, G Bwee; Pha Moers, Antoine; Hendriks, Sjon Na; Mendes, Odette; Griekspoor, Yvonne; Werten, Marc Wt; Schaap, Peter J; van der Oost, John; Eggink, Gerrit

    2017-07-01

    A Monascus ruber strain was isolated that was able to grow on mineral medium at high sugar concentrations and 175g/l lactic acid at pH 2.8. Its genome and transcriptomes were sequenced and annotated. Genes encoding lactate dehydrogenase (LDH) were introduced to accomplish lactic acid production and two genes encoding pyruvate decarboxylase (PDC) were knocked out to subdue ethanol formation. The strain preferred lactic acid to glucose as carbon source, which hampered glucose consumption and therefore also lactic acid production. Lactic acid consumption was stopped by knocking out 4 cytochrome-dependent LDH (CLDH) genes, and evolutionary engineering was used to increase the glucose consumption rate. Application of this strain in a fed-batch fermentation resulted in a maximum lactic acid titer of 190g/l at pH 3.8 and 129g/l at pH 2.8, respectively 1.7 and 2.2 times higher than reported in literature before. Yield and productivity were on par with the best strains described in literature for lactic acid production at low pH. Copyright © 2017 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

  17. Pink Salmon Distribution, Pacific Northwest (updated March, 2006)

    Data.gov (United States)

    Pacific States Marine Fisheries Commission — This dataset is a record of fish distribution and activity for PINK SALMON contained in the StreamNet database. This feature class was created based on linear event...

  18. Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil

    OpenAIRE

    Ting Yang; Lei Ren; Yang Jia; Shuanghu Fan; Junhuan Wang; Jiayi Wang; Ruth Nahurira; Haisheng Wang; Yanchun Yan

    2018-01-01

    Di-(2-ethylehxyl) phthalate (DEHP) is one of the most broadly representative phthalic acid esters (PAEs) used as a plasticizer in polyvinyl chloride (PVC) production, and is considered to be an endocrine-disrupting chemical. DEHP and its monoester metabolites are responsible for adverse effects on human health. An efficient DEHP-degrading bacterial strain Rhodococcus ruber YC-YT1, with super salt tolerance (0–12% NaCl), is the first DEHP-degrader isolated from marine plastic debris found in c...

  19. Individual or synchronous biodegradation of di-n-butyl phthalate and phenol by Rhodococcus ruber strain DP-2

    Energy Technology Data Exchange (ETDEWEB)

    He, Zhixing; Niu, Chengzhen; Lu, Zhenmei, E-mail: lzhenmei@zju.edu.cn

    2014-05-01

    Highlights: • A Rhodococcus ruber strain degraded DBP and phenol. • Degradation kinetics of DBP or phenol fit modified first-order models. • Degradation interaction between DBP and phenol was studied by strain DP-2. • The degradation genes transcriptional were quantified by RT-qPCR. - Abstract: The bacterial strain DP-2, identified as Rhodococcus ruber, is able to effectively degrade di-n-butyl phthalate (DBP) and phenol. Degradation kinetics of DBP and phenol at different initial concentrations revealed DBP and phenol degradation to fit modified first-order models. The half-life of DBP degradation ranged from 15.81 to 27.75 h and phenol degradation from 14.52 to 45.52 h under the initial concentrations of 600–1200 mg/L. When strain DP-2 was cultured with a mixture of DBP (800 mg/L) and phenol (700 mg/L), DBP degradation rate was found to be only slightly influenced; however, phthalic acid (PA) accumulated, and phenol degradation was clearly inhibited during synchronous degradation. Transcriptional levels of degradation genes, phenol hydroxylase (pheu) and phthalate 3,4-dioxygenase (pht), decreased significantly more during synchronous degradation than during individual degradation. Quantitative estimation of individual or synchronous degradation kinetics is essential to manage mixed hazardous compounds through biodegradation in industrial waste disposal.

  20. Pink line syndrome (PLS) in the scleractinian coral Porites lutea

    Digital Repository Service at National Institute of Oceanography (India)

    Ravindran, J.; Raghukumar, C.

    Reef sites Pink line syndrome (PLS) in the scleractinian coral Porites lutea Accepted: 10 May 2002 / Published online: 5 July 2002 C211 Springer-Verlag 2002 We describe here an unreport- ed diseased state of Porites lutea (Milne-Edwards and Haime...)ontheKavarattireefof the Lakshadweep group of is- lands (11C176 N; 71C176E). Pink line syndrome (PLS) causes partial mortality of the coral P. lutea around Kavaratti Island (Fig. 1), and about 10% of colonies were found to be af- fected by PLS. The dead patches were colonized by a...

  1. Drosophila pink1 is required for mitochondrial function and interacts genetically with parkin.

    Science.gov (United States)

    Clark, Ira E; Dodson, Mark W; Jiang, Changan; Cao, Joseph H; Huh, Jun R; Seol, Jae Hong; Yoo, Soon Ji; Hay, Bruce A; Guo, Ming

    2006-06-29

    Parkinson's disease is the second most common neurodegenerative disorder and is characterized by the degeneration of dopaminergic neurons in the substantia nigra. Mitochondrial dysfunction has been implicated as an important trigger for Parkinson's disease-like pathogenesis because exposure to environmental mitochondrial toxins leads to Parkinson's disease-like pathology. Recently, multiple genes mediating familial forms of Parkinson's disease have been identified, including PTEN-induced kinase 1 (PINK1; PARK6) and parkin (PARK2), which are also associated with sporadic forms of Parkinson's disease. PINK1 encodes a putative serine/threonine kinase with a mitochondrial targeting sequence. So far, no in vivo studies have been reported for pink1 in any model system. Here we show that removal of Drosophila PINK1 homologue (CG4523; hereafter called pink1) function results in male sterility, apoptotic muscle degeneration, defects in mitochondrial morphology and increased sensitivity to multiple stresses including oxidative stress. Pink1 localizes to mitochondria, and mitochondrial cristae are fragmented in pink1 mutants. Expression of human PINK1 in the Drosophila testes restores male fertility and normal mitochondrial morphology in a portion of pink1 mutants, demonstrating functional conservation between human and Drosophila Pink1. Loss of Drosophila parkin shows phenotypes similar to loss of pink1 function. Notably, overexpression of parkin rescues the male sterility and mitochondrial morphology defects of pink1 mutants, whereas double mutants removing both pink1 and parkin function show muscle phenotypes identical to those observed in either mutant alone. These observations suggest that pink1 and parkin function, at least in part, in the same pathway, with pink1 functioning upstream of parkin. The role of the pink1-parkin pathway in regulating mitochondrial function underscores the importance of mitochondrial dysfunction as a central mechanism of Parkinson's disease

  2. PELAKSANAAN KEGIATAN SPECIAL EVENT JAKARTA GOES PINK OLEH LOVEPINK INDONESIA

    Directory of Open Access Journals (Sweden)

    Nugroho Ajie Hartono

    2017-01-01

    Full Text Available ABSTRAKPenelitian ini bertujuan untuk mengetahui kegiatan special event yang dilakukan Lovepink Indonesiadalam menyelenggarakan Jakarta Goes Pink 2015 untuk meningkatkan kesadaran. Penelitian inimenggunakan Event Management Process Joe Goldblatt sebagai landasan konsep.Penelitian ini adalah penelitian kualitatif dengan pendekatan studi deskriptif. Teknik pengumpulan datadilakukan dengan wawancara mendalam, observasi partisipan pasif, dan studi kepustakaan, dengan teknik pengumpulan key informan purposive sampling. Teknik analisis data menggunakan tiga tahap yaitu reduksi data, penyajian data, serta penarikan kesimpulan. Teknik validitas data menggunakantriangulasi sumber data.Hasil penelitian ini mengemukakan bahwa manajemen special event Jakarta Goes Pink dikategorikandalam riset, desain, perencanaan, koordinasi, dan evaluasi. Riset yang dilakukan meliputi analisissituasi terkait kesadaran masyarakat Indonesia tentang kanker payudara, hasil riset menyatakanbahwa kesadaran masih rendah, terutama dibandingkan dengan aktivitas Pink Ribbon diluar negeridan evaluasi acara tahun sebelumnya. Desain acara dilakukan menggunakan unsur warna untukmemerahmudakan Jakarta, edukasi pengunjung terhadap kanker payudara, dan unsur hiburan dengankonsep fair and festival. Perencanaan Jakarta Goes Pink meliputi penentuan tujuan yang kemudiandapat menentukan tanggal dan lokasi, penetapan anggaran, pembagian tugas pengurus, dan publikasiyang dilakukan melalui media sosial dan bantuan mitra media. Koordinasi dilakukan sebagai upayapengelolaan komunikasi antara pihak eksternal seperti komunitas, relawan, sponsor, dan mitra media;serta pihak internal yaitu kepengurusan Jakarta Goes Pink. Tahap evaluasi yang dilakukan JakartaGoes Pink meliputi evaluasi acara, feedback langsung dari orang terdekat, dan penghitungan jumlahliputan media massa dan sifat pemberitaan.Kata kunci: Special event, kesadaran, organisasi, kanker payudara, event management process ABSTRACTThis study

  3. The Color Pink Is Bad for Fighting Breast Cancer

    NARCIS (Netherlands)

    S. Puntoni (Stefano)

    2011-01-01

    textabstractThe article presents an interview with researcher Stefano Puntoni, who conducted experiments indicating that the color pink makes women think they have a reduced susceptibility to getting cancer, and also disinclines them toward donating to cancer research. Topics discussed include why

  4. Sample sufficiency of chinese pink grown in different substrates

    Directory of Open Access Journals (Sweden)

    Sidinei José lopes

    2016-04-01

    Full Text Available The cravina is an excellent plant to build up gardens due to its early flowering, abundant flowering and great performance in spring and autumn. The objective was to estimate the sample size for plant chinese pink, grown on different substrates, and check the variability of the sample size between growth parameters and production and substrates. They used seven treatments (substrates: S1 = 50% soil + 50% rice husk ash; S2 = 80% soil + 20% earthworm castings; S3 = 80% rice husk ash + 20% earthworm castings; S4 = 40% soil + 40% rice husk ash + 20% earthworm castings; S5 = 100% peat; S6 = 100% commercial substrate Mecplant®; S7 = 50% peat + 50% rice husk ash, with 56 repetitions each, totaling 392 plants of garden pink, which was evaluated in 17 of growth and production parameters. The methodology used to bootstrap resampling, with replacement, for each character within each substrate with predetermined error: 5, 10, 20 and 40% of the average (D%. To a 95% confidence interval, with D = 20%, the substrate 50% soil and 50% of rice husk ash had the largest sample size 11 characters; when comparing the characters , the number of flower buds had the highest sample size on average 113 plants. Using samples of 44 plant chinese pink for commercial substrate Mecplant® meet the lower precisions or equal to 20% for all variables. There is variation in sample size in relation to the substrate used and the variable evaluated in chinese pink plants.

  5. Lavaka lõpetajate pink on pikk / Tiiu Laks

    Index Scriptorium Estoniae

    Laks, Tiiu, 1984-

    2009-01-01

    Kevadel lavakunstikooli lõpetavate tudengite lavastusest "Pikk pink" , mis koosneb kuuest lühiloost - Mait Jooritsa "Kantani padi", Kristjan Üksküla "Jääger" ja "Albioni tütar", Roland Laosi "Genees ja katastroof", Hendrik Toompere jun. jun. "Karupoeg Puhh" ja Kerttu Moppeli " Leib!". Etendused toimuvad Hobuveskis

  6. Pink läks pikemaks... / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2007-01-01

    20. sept. VAT Teatris esietendunud Eesti Muusika ja Teatriakadeemia Lavakunstikooli lavastajate õppesuuna tudengite koolitööst "Pink", mis koosneb kahest iseseisvast loost - Jon Fosse "Talv", lavastaja Robert Annus ja Edward Albee "Loomaaialugu", lavastaja Uku Uusberg. Lisaks küsimus "Aare Toikka, kuidas noored lavastajatudengid VAT Teatri tiiva alla sattusid?"

  7. BST-PINK PANTHER. An intelligent CAMAC crate controller

    International Nuclear Information System (INIS)

    Troester, D.A.

    1984-01-01

    A technical and functional description of the PINK system for intelligent, distributed data acquisition, data formatting, and data reduction is presented. The system has been developed to bypass some of the constraints of CAMAC when collecting data with the high-resolution π 0 spectrometers of the Basel-Stockholm-Thessaloniki (BST) Collaboration at CERN. (orig.)

  8. Potential for the development of tolerance by Aspergillus amstelodami, A. repens and A. ruber after repeated exposure to potassium sorbate.

    Science.gov (United States)

    Viñas, I; Morlans, I; Sanchis, V

    1990-01-01

    Three strains of A. amstelodami, A. repens and A. ruber were exposed to various levels of potassium sorbate, and the MICs were determined. Selected strains of the molds were then repeatedly exposed to subinhibitory levels of the compound to determine whether increased tolerance might develop. The MIC of sorbate (pH 5.5 or 6.5) for 3 species of Aspergillus was 0.07%. Increasing levels of sorbate resulted in increasing growth suppression of the molds. The 3 Aspergillus species were tested for increased tolerance to potassium sorbate, and none was found. They developed a slight increase in tolerance dependent upon pH and the mold strain by subculturing at low levels of sorbate.

  9. Biodegradation of Di-(2-ethylhexyl Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil

    Directory of Open Access Journals (Sweden)

    Ting Yang

    2018-05-01

    Full Text Available Di-(2-ethylehxyl phthalate (DEHP is one of the most broadly representative phthalic acid esters (PAEs used as a plasticizer in polyvinyl chloride (PVC production, and is considered to be an endocrine-disrupting chemical. DEHP and its monoester metabolites are responsible for adverse effects on human health. An efficient DEHP-degrading bacterial strain Rhodococcus ruber YC-YT1, with super salt tolerance (0–12% NaCl, is the first DEHP-degrader isolated from marine plastic debris found in coastal saline seawater. Strain YC-YT1 completely degraded 100 mg/L DEHP within three days (pH 7.0, 30 °C. According to high-performance liquid chromatography–mass spectrometry (HPLC-MS analysis, DEHP was transformed by strain YC-YT1 into phthalate (PA via mono (2-ethylehxyl phthalate (MEHP, then PA was used for cell growth. Furthermore, YC-YT1 metabolized initial concentrations of DEHP ranging from 0.5 to 1000 mg/L. Especially, YC-YT1 degraded up to 60% of the 0.5 mg/L initial DEHP concentration. Moreover, compared with previous reports, strain YC-YT1 had the largest substrate spectrum, degrading up to 13 kinds of PAEs as well as diphenyl, p-nitrophenol, PA, benzoic acid, phenol, protocatechuic acid, salicylic acid, catechol, and 1,2,3,3-tetrachlorobenzene. The excellent environmental adaptability of strain YC-YT1 contributed to its ability to adjust its cell surface hydrophobicity (CSH so that 79.7–95.9% of DEHP-contaminated agricultural soil, river water, coastal sediment, and coastal seawater were remedied. These results demonstrate that R. ruber YC-YT1 has vast potential to bioremediate various DEHP-contaminated environments, especially in saline environments.

  10. S-Nitrosylation of PINK1 Attenuates PINK1/Parkin-Dependent Mitophagy in hiPSC-Based Parkinson’s Disease Models

    Directory of Open Access Journals (Sweden)

    Chang-Ki Oh

    2017-11-01

    Full Text Available Mutations in PARK6 (PINK1 and PARK2 (Parkin are linked to rare familial cases of Parkinson’s disease (PD. Mutations in these genes result in pathological dysregulation of mitophagy, contributing to neurodegeneration. Here, we report that environmental factors causing a specific posttranslational modification on PINK1 can mimic these genetic mutations. We describe a molecular mechanism for impairment of mitophagy via formation of S-nitrosylated PINK1 (SNO-PINK1. Mitochondrial insults simulating age- or environmental-related stress lead to increased SNO-PINK1, inhibiting its kinase activity. SNO-PINK1 decreases Parkin translocation to mitochondrial membranes, disrupting mitophagy in cell lines and human-iPSC-derived neurons. We find levels of SNO-PINK1 in brains of α-synuclein transgenic PD mice similar to those in cell-based models, indicating the pathophysiological relevance of our findings. Importantly, SNO-PINK1-mediated deficits in mitophagy contribute to neuronal cell death. These results reveal a direct molecular link between nitrosative stress, SNO-PINK1 formation, and mitophagic dysfunction that contributes to the pathogenesis of PD.

  11. The yeast complex I equivalent NADH dehydrogenase rescues pink1 mutants.

    Directory of Open Access Journals (Sweden)

    Sven Vilain

    2012-01-01

    Full Text Available Pink1 is a mitochondrial kinase involved in Parkinson's disease, and loss of Pink1 function affects mitochondrial morphology via a pathway involving Parkin and components of the mitochondrial remodeling machinery. Pink1 loss also affects the enzymatic activity of isolated Complex I of the electron transport chain (ETC; however, the primary defect in pink1 mutants is unclear. We tested the hypothesis that ETC deficiency is upstream of other pink1-associated phenotypes. We expressed Saccaromyces cerevisiae Ndi1p, an enzyme that bypasses ETC Complex I, or sea squirt Ciona intestinalis AOX, an enzyme that bypasses ETC Complex III and IV, in pink1 mutant Drosophila and find that expression of Ndi1p, but not of AOX, rescues pink1-associated defects. Likewise, loss of function of subunits that encode for Complex I-associated proteins displays many of the pink1-associated phenotypes, and these defects are rescued by Ndi1p expression. Conversely, expression of Ndi1p fails to rescue any of the parkin mutant phenotypes. Additionally, unlike pink1 mutants, fly parkin mutants do not show reduced enzymatic activity of Complex I, indicating that Ndi1p acts downstream or parallel to Pink1, but upstream or independent of Parkin. Furthermore, while increasing mitochondrial fission or decreasing mitochondrial fusion rescues mitochondrial morphological defects in pink1 mutants, these manipulations fail to significantly rescue the reduced enzymatic activity of Complex I, indicating that functional defects observed at the level of Complex I enzymatic activity in pink1 mutant mitochondria do not arise from morphological defects. Our data indicate a central role for Complex I dysfunction in pink1-associated defects, and our genetic analyses with heterologous ETC enzymes suggest that Ndi1p-dependent NADH dehydrogenase activity largely acts downstream of, or in parallel to, Pink1 but upstream of Parkin and mitochondrial remodeling.

  12. Three Pink Decades: Breast Cancer Coverage in Magazine Advertisements.

    Science.gov (United States)

    AbiGhannam, Niveen; Chilek, Lindsay A; Koh, Hyeseung E

    2018-04-01

    Breast cancer advocacy has experienced tremendous success since the 1980s. Yet, the quality and authenticity of breast cancer information in the media are sometimes questionable. Using a content analysis, we examined the informative (donation information, breast cancer advocacy content, etc.) and persuasive (appeals used, cues to action, etc.) contents of magazine advertisements relevant to breast cancer. While ads offered minimal informative content about the disease or about ways by which sales will contribute to the breast cancer cause, they integrated "breast cancer appeals," such as the color pink, the pink ribbon, and mostly positive depictions of survivorship and hope, into the ads. Breast cancer thus took center stage in the persuasive content of the ads, but a back seat when it came to their informative content. We discuss the implications of those findings in light of the meanings and purposes of cause-related marketing campaigns.

  13. Time response measurements of pressure sensors using pink noise technique

    International Nuclear Information System (INIS)

    Pereira, Iraci Martinez; Santos, Roberto Carlos dos

    2009-01-01

    This work presents an experimental setup for Pink Noise method application on pressure transmitters' response times. The Pink Noise method consists on injecting artificial pressure noise into the pressure transmitter. The artificial pressure noise is generated using a current-to-pressure (I-to-P) converter, which is driven by a random noise signal generator. The output pressure transmitter noise is then analyzed using conventional Noise Analysis Technique. Noise signals may be interpreted using spectral techniques or empirical time series models. The frequency domain method consists of evaluating the Power Spectral Density (PSD) function. The information needed for time constant estimation can be obtained by fitting an all-pole transfer function to this power spectral density. (author)

  14. Pink Globalization: Hello Kitty sebagai Instrumen Soft-Diplomacy Jepang

    OpenAIRE

    Rijal, Najamuddin Khairur

    2017-01-01

    Pink globalization merujuk pada istilah untuk menggambarkan popularitas internasional Hello Kitty melalui penyebaran berbagai jenis produk berlabel Hello Kitty yang kawaii dari Jepang sebagai bagian dari dunia industri. Popularitas Hello Kitty tersebut merupakan soft-power yang memberikan kekuatan nasional tersendiri Jepang. Jepang kemudian menjadikan Hello Kitty sebagai instrumen soft-diplomacy sebagai upaya meningkatkan citra pariwisata dan menarik wisatawan untuk berkunjung ke Jepang. Tuli...

  15. The Time is Gone!? Pink Floyds "Time" (1973)

    OpenAIRE

    Hantschel, Florian

    2012-01-01

    This paper provides a hermeneutic approach on Pink Floyds popular song "Time" from 1973s album Dark Side Of The Moon. The primary aim of this examination is the analysis of the conceptual connection of music and lyrics in this song. Moreover, derivation of semantic meaning potential is used to form an intersubjectively verifiable song interpretation. All in all, the main results of this analysis suggest a close connection of the songs musical parameters, i.e. form, harmony, melody, rhythm as ...

  16. The mitochondrial fusion-promoting factor mitofusin is a substrate of the PINK1/parkin pathway.

    Directory of Open Access Journals (Sweden)

    Angela C Poole

    2010-04-01

    Full Text Available Loss-of-function mutations in the PINK1 or parkin genes result in recessive heritable forms of parkinsonism. Genetic studies of Drosophila orthologs of PINK1 and parkin indicate that PINK1, a mitochondrially targeted serine/threonine kinase, acts upstream of Parkin, a cytosolic ubiquitin-protein ligase, to promote mitochondrial fragmentation, although the molecular mechanisms by which the PINK1/Parkin pathway promotes mitochondrial fragmentation are unknown. We tested the hypothesis that PINK1 and Parkin promote mitochondrial fragmentation by targeting core components of the mitochondrial morphogenesis machinery for ubiquitination. We report that the steady-state abundance of the mitochondrial fusion-promoting factor Mitofusin (dMfn is inversely correlated with the activity of PINK1 and Parkin in Drosophila. We further report that dMfn is ubiquitinated in a PINK1- and Parkin-dependent fashion and that dMfn co-immunoprecipitates with Parkin. By contrast, perturbations of PINK1 or Parkin did not influence the steady-state abundance of the mitochondrial fission-promoting factor Drp1 or the mitochondrial fusion-promoting factor Opa1, or the subcellular distribution of Drp1. Our findings suggest that dMfn is a direct substrate of the PINK1/Parkin pathway and that the mitochondrial morphological alterations and tissue degeneration phenotypes that derive from mutations in PINK1 and parkin result at least in part from reduced ubiquitin-mediated turnover of dMfn.

  17. Loss of PINK1 increases the heart's vulnerability to ischemia-reperfusion injury.

    Directory of Open Access Journals (Sweden)

    Hilary K Siddall

    Full Text Available Mutations in PTEN inducible kinase-1 (PINK1 induce mitochondrial dysfunction in dopaminergic neurons resulting in an inherited form of Parkinson's disease. Although PINK1 is present in the heart its exact role there is unclear. We hypothesized that PINK1 protects the heart against acute ischemia reperfusion injury (IRI by preventing mitochondrial dysfunction.Over-expressing PINK1 in HL-1 cardiac cells reduced cell death following simulated IRI (29.2±5.2% PINK1 versus 49.0±2.4% control; N = 320 cells/group P5 animals/group; P<0.05. Cardiomyocytes isolated from PINK1-/- hearts had a lower resting mitochondrial membrane potential, had inhibited mitochondrial respiration, generated more oxidative stress during simulated IRI, and underwent rigor contracture more rapidly in response to an uncoupler when compared to PINK1+/+ cells suggesting mitochondrial dysfunction in hearts deficient in PINK1.We show that the loss of PINK1 increases the heart's vulnerability to ischemia-reperfusion injury. This may be due, in part, to increased mitochondrial dysfunction. These findings implicate PINK1 as a novel target for cardioprotection.

  18. The Effect of Pink Uniform Color on Future Imaging among Female Student Nurses

    OpenAIRE

    石井, 国雄; 加藤, 樹里; 田戸岡, 好香

    2017-01-01

    Responding to various social demands, variety of nurse uniforms have been created and worn. The present study examined the effects of pink nursing uniform on self-perception and work motivation among female student nurses. We hypothesized that female student nurses would perceive themselves more competent and increase work motivation in the pink condition. In the experiment, Japanese female student nurses imaged their future career as nurse, watching the illustration of nurse either in a pink...

  19. Pretty in pink: The early development of gender-stereotyped colour preferences.

    Science.gov (United States)

    Lobue, Vanessa; Deloache, Judy S

    2011-09-01

    Parents commonly dress their baby girls in pink and their baby boys in blue. Although there is research showing that children prefer the colour blue to other colours (regardless of gender), there is no evidence that girls actually have a special preference for the colour pink. This is the focus of the current investigation. In a large cross-sectional study, children aged 7 months to 5 years were offered eight pairs of objects and asked to choose one. In every pair, one of the objects was always pink. By the age of 2, girls chose pink objects more often than boys did, and by the age of 2.5, they had a significant preference for the colour pink over other colours. At the same time, boys showed an increasing avoidance of pink. These results thus reveal that sex differences in young children's preference for the colour pink involves both an increasing attraction to pink by young girls and a growing avoidance of pink by boys. © 2011 The British Psychological Society.

  20. Responses of pink salmon to CO2-induced aquatic acidification

    Science.gov (United States)

    Ou, Michelle; Hamilton, Trevor J.; Eom, Junho; Lyall, Emily M.; Gallup, Joshua; Jiang, Amy; Lee, Jason; Close, David A.; Yun, Sang-Seon; Brauner, Colin J.

    2015-10-01

    Ocean acidification negatively affects many marine species and is predicted to cause widespread changes to marine ecosystems. Similarly, freshwater ecosystems may potentially be affected by climate-change-related acidification; however, this has received far less attention. Freshwater fish represent 40% of all fishes, and salmon, which rear and spawn in freshwater, are of immense ecosystem, economical and cultural importance. In this study, we investigate the impacts of CO2-induced acidification during the development of pink salmon, in freshwater and following early seawater entry. At this critical and sensitive life stage, we show dose-dependent reductions in growth, yolk-to-tissue conversion and maximal O2 uptake capacity; as well as significant alterations in olfactory responses, anti-predator behaviour and anxiety under projected future increases in CO2 levels. These data indicate that future populations of pink salmon may be at risk without mitigation and highlight the need for further studies on the impact of CO2-induced acidification on freshwater systems.

  1. An overlooked pink species of land iguana in the Galapagos.

    Science.gov (United States)

    Gentile, Gabriele; Fabiani, Anna; Marquez, Cruz; Snell, Howard L; Snell, Heidi M; Tapia, Washington; Sbordoni, Valerio

    2009-01-13

    Despite the attention given to them, the Galápagos have not yet finished offering evolutionary novelties. When Darwin visited the Galápagos, he observed both marine (Amblyrhynchus) and land (Conolophus) iguanas but did not encounter a rare pink black-striped land iguana (herein referred to as "rosada," meaning "pink" in Spanish), which, surprisingly, remained unseen until 1986. Here, we show that substantial genetic isolation exists between the rosada and syntopic yellow forms and that the rosada is basal to extant taxonomically recognized Galápagos land iguanas. The rosada, whose present distribution is a conundrum, is a relict lineage whose origin dates back to a period when at least some of the present-day islands had not yet formed. So far, this species is the only evidence of ancient diversification along the Galápagos land iguana lineage and documents one of the oldest events of divergence ever recorded in the Galápagos. Conservation efforts are needed to prevent this form, identified by us as a good species, from extinction.

  2. Biochemical and chemical characterization of pink-pigmented oxidative bacteria.

    Science.gov (United States)

    Wallace, P L; Hollis, D G; Weaver, R E; Moss, C W

    1990-04-01

    The biochemical and chemical characteristics were determined for 156 clinical isolates of pink-pigmented bacteria that are similar to but distinct from Methylobacterium extorquens (synonymous with Pseudomonas mesophilica). These isolates were gram-negative, nonfermentative, usually nonvacuolated, coccoid rods; all grew at 35 degrees C and were catalase and urease positive; the majority grew on MacConkey agar and were variable for oxidase production and motility. On the basis of oxidation of xylose and mannitol and hydrolysis of esculin, these 156 strains were subdivided into four groups that were designated "pink coccoid" groups I, II, III, and IV. Groups I, II, and III are similar to an unnamed taxon described by Gilardi and Faur in 1984; only strains of group IV hydrolyze esculin. The cellular fatty acid compositions of strains of groups I, II, and III were essentially identical and differed from strains of group IV by the absence of 3-OH-C14:0 and the presence of C19:0 delta and 2-OH-C19:0 delta. The fatty acid composition of group IV strains was most similar to that of M. extorquens but differed by the presence of small amounts of two C17:1 acids, 3-OH-C16:0, and 2-OH-C18:1.

  3. Plant colonization by pink-pigmented facultative methylotrophic bacteria (PPFMs).

    Science.gov (United States)

    Omer, Zahra S; Tombolini, Riccardo; Gerhardson, Berndt

    2004-03-01

    Bacteria belonging to the genus Methylobacterium are characterized by being able to rely on methanol as a sole carbon and energy source and by presenting a more or less intense pink reddish pigmentation. These bacteria, also referred to as pink-pigmented methylotrophic bacteria (PPFMs), are common inhabitants of the phyllosphere and are found in many other environmental samples. Since they grow slowly they are often overlooked and their impact on phyllosphere microbial communities and on the plants harboring them is not well studied nor has their ecology been elucidated. In a survey of PPFM colonization in three different agricultural sites, PPFM populations were identified on both red clover and winter wheat, but red clover was more consistently colonized. Isolations from collected leaves showed PPFM populations to decrease from spring towards summer, but they increased again towards the end of the cropping season. Isolates from red clover readily colonized winter wheat leaves and vice versa in greenhouse experiments, but population sizes were dependent on the application procedure. Tested isolates had also good potential to colonize the rhizosphere, especially after seed inoculations. Confocal scanning laser microscopy showed gfp-tagged isolates to colonize the surface of clover leaves by forming large aggregates.

  4. Evaluation of induced color changes in chicken breast meat during simulation of pink color defect.

    Science.gov (United States)

    Holownia, K; Chinnan, M S; Reynolds, A E; Koehler, P E

    2003-06-01

    The objective of the study was to establish a pink threshold and simulate the pink defect in cooked chicken breast meat with treatment combinations that would induce significant changes in the color of raw and cooked meat. The subjective pink threshold used in judging pink discoloration was established at a* = 3.8. Samples of three color groups (normal, lighter than normal, and darker than normal) of boneless, skinless chicken breast muscles were selected based on instrumental color values. The in situ changes were induced using sodium chloride, sodium tripolyphosphate, sodium erythorbate, and sodium nitrite at two levels: present and not present. Fillets in all treatments were subjected to individual injections, followed by tumbling, cooking, and chilling. Samples were analyzed for color [lightness (L*), red/green axis (a*), yellow/blue axis (b*)] and reflectance spectra. Simulation of the pink defect was achieved in eight of the 16 treatment combinations when sodium nitrite was present and in an additional two treatment combinations when it was absent. Pinking in cooked samples was affected (P meat color. Results confirmed that it was possible to simulate the undesired pinking in cooked chicken white meat when in situ conditions were induced by sodium chloride, sodium tripolyphosphate, and sodium nitrite. The continuation of the simulation study can aid in developing alternative processing methods to eliminate potential pink defects.

  5. Revisiting the Pink Triangle Exercise: An Exploration of Experiential Learning in Graduate Social Work Education

    Science.gov (United States)

    Pugh, Greg L.

    2014-01-01

    The pink triangle exercise is an example of an experiential learning exercise that creates cognitive dissonance and deep learning of unrealized internalized biases among social work students. Students wear a button with a pink triangle on it for 1 day and write a reflection paper. The exercise increases self-awareness, cultural competence, and the…

  6. Progression of subtle motor signs in PINK1 mutation carriers with mild dopaminergic deficit

    DEFF Research Database (Denmark)

    Eggers, C; Schmidt, A; Hagenah, J

    2010-01-01

    While homozygous mutations in the PINK1 gene cause recessively inherited early-onset Parkinson disease (PD), heterozygous mutations have been suggested as a susceptibility factor.......While homozygous mutations in the PINK1 gene cause recessively inherited early-onset Parkinson disease (PD), heterozygous mutations have been suggested as a susceptibility factor....

  7. Early-onset parkinsonism associated with PINK1 mutations: frequency, genotypes, and phenotypes.

    NARCIS (Netherlands)

    Bonifati, V.; Rohe, C.F.; Breedveld, G.J.; Fabrizio, E.; Mari, M. De; Tassorelli, C.; Tavella, A.; Marconi, R.; Nicholl, D.; Chien, H.F.; Fincati, E.; Abbruzzese, G.; Marini, P.; Gaetano, A. De; Horstink, M.W.I.M.; Maat-Kievit, J.A.; Sampaio, C.; Antonini, A.; Stocchi, F.; Montagna, P.; Toni, V.; Guidi, M.; Dalla Libera, A.; Tinazzi, M.; Pandis, F. De; Goldwurm, S.; Klein, A. de; Barbosa, E.; Lopiano, L.; Martignoni, E.; Lamberti, P.; Vanacore, N.; Meco, G.; Oostra, B.A.

    2005-01-01

    OBJECTIVE: To assess the prevalence, nature, and associated phenotypes of PINK1 gene mutations in a large series of patients with early-onset (<50 years) parkinsonism. METHODS: The authors studied 134 patients (116 sporadic and 18 familial; 77% Italian) and 90 Italian controls. The whole PINK1

  8. Phenology of body mass changes during reproduction in a nomadic, tropical waterbird, the Scarlet Ibis (Eudocimus ruber).

    Science.gov (United States)

    Babbitt, Gregory A; Frederick, Peter C

    2008-09-01

    In birds, the prereproductive buildup of endogenous energy reserves (e.g. body fat) is highly variable and is often thought to be a strategy evolving in response to either seasonal and/or unpredictable changes in breeding conditions. Nomadic behavior is also thought to be an adaptation to unpredictable resource distribution in both space and time. Because of the difficultly in obtaining a longitudinal time series of body masses for free-living individuals of highly nomadic species, the relationship between nomadism and endogenous energy storage has not been explored. In this study, we investigated prereproductive energy storage in a large free-flighted captive colony of highly nomadic waterbird, the Scarlet Ibis, Eudocimus ruber. We used size-corrected body mass as an index of body condition both earlier to and during breeding. We compared both breeders and nonbreeders body condition earlier to nesting. We also prevented a subsample of the birds from gaining mass earlier to nesting and compared their nesting success with a control group that was allowed to feed freely. Although significant differences were found in prereproductive body conditions of breeders and nonbreeders, we were unable to control breeding by manipulating prereproductive condition, most likely because of the ability of some birds to rapidly change body condition within several days or weeks earlier to nesting. We conclude that prereproductive energy storage is important for nesting success in both sexes of this highly nomadic species, however energy stores are highly labile and can be rapidly obtained through prenesting hyperphagia. Zoo Biol 27:360-370, 2008. (c) 2008 Wiley-Liss, Inc.

  9. Yeasts from Scarlet ibises (Eudocimus ruber): A focus on monitoring the antifungal susceptibility of Candida famata and closely related species.

    Science.gov (United States)

    Brilhante, Raimunda Sâmia Nogueira; Silva, Aline Lobão da; Monteiro, Frederico Ozanan Barros; Guedes, Glaucia Morgana de Melo; Sales, Jamille Alencar; Oliveira, Jonathas Sales de; Maia Junior, José Erisvaldo; Miranda, Stefânia Araújo; Sidrim, José Júlio Costa; Alencar, Lucas Pereira de; Castelo-Branco, Débora Souza Collares Maia; Cordeiro, Rossana de Aguiar; Pereira Neto, Waldemiro de Aquino; Rocha, Marcos Fábio Gadelha

    2017-10-01

    This study aimed to identify yeasts from the gastrointestinal tract of scarlet ibises (Eudocimus ruber) and from plant material collected from the environment where they live. Then, the isolates phenotypically identified as Candida famata were submitted to molecular identification of their closely related species and evaluated for their antifungal susceptibility and possible resistance mechanisms to antifungal drugs. Cloacal swabs from 20 scarlet ibises kept in captivity at Mangal das Garças Park (Brazil), pooled stool samples (n = 20) and samples of trunks and hollow of trees (n = 20) obtained from their enclosures were collected. The samples were seeded on Sabouraud agar supplemented with chloramphenicol. The 48 recovered isolates were phenotypically identified as 15 Candida famata, 13 Candida catenulata, 2 Candida intermedia, 1 Candida lusitaniae, 2 Candida guilliermondii, 1 Candida kefyr, 1 Candida amapae, 1 Candida krusei, 8 Trichosporon spp., and 4 Rhodotorula spp. The C. famata isolates were further identified as 3 C. famata, 8 Debaryomyces nepalensis, and 4 C. palmioleophila. All C. famata and C. palmioleophila were susceptible to caspofungin and itraconazole, while one D. nepalensis was resistant to fluconazole and voriconazole. This same isolate and another D. nepalensis had lower amphotericin B susceptibility. The azole resistant strain had an increased efflux of rhodamine 6G and an alteration in the membrane sterol content, demonstrating multifactorial resistance mechanism. Finally, this research shows that scarlet ibises and their environment harbor C. famata and closely related species, including antifungal resistant isolates, emphasizing the need of monitoring the antifungal susceptibility of these yeast species. © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  10. PINK1 is necessary for long term survival and mitochondrial function in human dopaminergic neurons.

    Directory of Open Access Journals (Sweden)

    Alison Wood-Kaczmar

    2008-06-01

    Full Text Available Parkinson's disease (PD is a common age-related neurodegenerative disease and it is critical to develop models which recapitulate the pathogenic process including the effect of the ageing process. Although the pathogenesis of sporadic PD is unknown, the identification of the mendelian genetic factor PINK1 has provided new mechanistic insights. In order to investigate the role of PINK1 in Parkinson's disease, we studied PINK1 loss of function in human and primary mouse neurons. Using RNAi, we created stable PINK1 knockdown in human dopaminergic neurons differentiated from foetal ventral mesencephalon stem cells, as well as in an immortalised human neuroblastoma cell line. We sought to validate our findings in primary neurons derived from a transgenic PINK1 knockout mouse. For the first time we demonstrate an age dependent neurodegenerative phenotype in human and mouse neurons. PINK1 deficiency leads to reduced long-term viability in human neurons, which die via the mitochondrial apoptosis pathway. Human neurons lacking PINK1 demonstrate features of marked oxidative stress with widespread mitochondrial dysfunction and abnormal mitochondrial morphology. We report that PINK1 plays a neuroprotective role in the mitochondria of mammalian neurons, especially against stress such as staurosporine. In addition we provide evidence that cellular compensatory mechanisms such as mitochondrial biogenesis and upregulation of lysosomal degradation pathways occur in PINK1 deficiency. The phenotypic effects of PINK1 loss-of-function described here in mammalian neurons provides mechanistic insight into the age-related degeneration of nigral dopaminergic neurons seen in PD.

  11. Oxidative stress and regulation of Pink1 in zebrafish (Danio rerio.

    Directory of Open Access Journals (Sweden)

    Madhusmita Priyadarshini

    Full Text Available Oxidative stress-mediated neuronal dysfunction is characteristic of several neurodegenerative disorders, including Parkinson's disease (PD. The enzyme tyrosine hydroxylase (TH catalyzes the formation of L-DOPA, the rate-limiting step in the biosynthesis of dopamine. A lack of dopamine in the striatum is the most characteristic feature of PD, and the cause of the most dominant symptoms. Loss of function mutations in the PTEN-induced putative kinase (PINK1 gene cause autosomal recessive PD. This study explored the basic mechanisms underlying the involvement of pink1 in oxidative stress-mediated PD pathology using zebrafish as a tool. We generated a transgenic line, Tg(pink1:EGFP, and used it to study the effect of oxidative stress (exposure to H2O2 on pink1 expression. GFP expression was enhanced throughout the brain of zebrafish larvae subjected to oxidative stress. In addition to a widespread increase in pink1 mRNA expression, mild oxidative stress induced a clear decline in tyrosine hydroxylase 2 (th2, but not tyrosine hydroxylase 1 (th1 expression, in the brain of wild-type larvae. The drug L-Glutathione Reduced (LGR has been associated with anti-oxidative and possible neuroprotective properties. Administration of LGR normalized the increased fluorescence intensity indicating pink1 transgene expression and endogenous pink1 mRNA expression in larvae subjected to oxidative stress by H2O2. In the pink1 morpholino oliogonucleotide-injected larvae, the reduction in the expression of th1 and th2 was partially rescued by LGR. The pink1 gene is a sensitive marker of oxidative stress in zebrafish, and LGR effectively normalizes the consequences of mild oxidative stress, suggesting that the neuroprotective effects of pink1 and LGR may be significant and useful in drug development.

  12. Hard X-ray emission spectroscopy with pink beam

    Energy Technology Data Exchange (ETDEWEB)

    Kvashnina, Kristina O.; Rossberg, Andre; Exner, Joerg; Scheinost, Andreas C. [Helmholtz-Zentrum Dresden-Rossendorf e.V., Dresden (Germany). Molecular Structures

    2017-06-01

    Valence-band X-ray emission spectroscopy (XES) with a ''pink beam'', i.e. a beam with large energy bandwidth produced by a double-multilayer monochromator, is introduced here to overcome the weak count rate of monochromatic beams produced by conventional double-crystal monochromators. Our results demonstrate that - in spite of the large bandwidth in the order of 100 eV - the high spectral resolution of the Johann-type spectrometer is maintained, while the two orders of magnitude higher flux greatly reduces the required counting time. The short working distance Johann-type X-ray emission spectrometer and multilayer monochromator is available at ROBL.

  13. You say "lean finely textured beef," I say "pink slime".

    Science.gov (United States)

    Reid, Rita-Marie Cain

    2014-01-01

    In 2012, American Broadcasting Companies, Inc. (ABC) broadcast a segment on its evening news show regarding the manufacture of "lean finely textured beef" by Beef Products, Inc. (BPI). The broadcast, as well as follow-up reports and social media communications, repeatedly referred to the product as "pink slime," a term originated by a United States Department of Agriculture (USDA) employee for the processed meat. The market backlash against the product was immediate and intense. Ultimately, BPI closed three processing plants, cut hundreds of jobs, and filed for bankruptcy. BPI sued ABC and others for food libel, defamation, and tortious interference. This research analyzes those claims and defenses and discusses the future of such cases.

  14. Pink esthetics in periodontics - Gingival depigmentation: A case series

    Directory of Open Access Journals (Sweden)

    Arthiie Thangavelu

    2012-01-01

    Full Text Available Smile expresses a feeling of joy, success, sensuality, affection, and courtesy, and reveals self-confidence and kindness. The harmony of the smile is determined not only by the shape, the position, and the color of the teeth, but also by the gingival tissues. Although melanin pigmentation of the gingiva is completely benign and does not present a medical problem, complaints of "black gums" are common, particularly in patients having a very high smile line. Thus, perio-esthetic treatment modalities strive to achieve a harmonious inter-relationship of the pink with white, which is imperative of all treatment procedures. For depigmentation of gingival, different treatment modalities have been reported, such as bur abrasion, scraping, partial thickness flap, cryotherapy, electrosurgery, and laser. In the present case series, scraping, electrosurgery, and diode laser have been tried for depigmentation, which are simple, effective, and yield good results, along with good patient satisfaction.

  15. Diatoms as Proxies for Abrupt Events in the Hudson River Estuary

    Science.gov (United States)

    Skorski, W.; Abbott, D. H.; Recasens, C.; Breger, D. L.

    2014-12-01

    The Hudson River estuary has been subject to many abrupt events throughout its history including hurricanes, droughts and pluvials. Hurricanes in particular are rare, discrete events that if fingerprinted can be used to develop better age models for Hudson River sediments. Proxies use observed physical characteristics or biological assemblages (e.g. diatom and foraminiferal assemblages) as tools to reconstruct past conditions prior to the modern instrumental record. Using a sediment core taken from the Hudson River (CDO2-29A), in New York City, drought and pluvial layers were selected based on Cs-137 dating while hurricane layers were determined from occurrences of tropical to subtropical foraminifera. Contrary to previous studies (Weaver, 1970, Weiss et al, 1978), more than sixty different diatom species have been identified using a scanning electron microscope (SEM). Cosmopolitan, hurricane and drought assemblages have begun to be identified after observing multiple layers (Table 1). Tropical foraminifera dominated by Globigerinoides ruber pink were also found in a hurricane layer that we infer was deposited during Hurricane Belle in 1976. More diatom abundance analyses and cataloged SEM pictures will provide further insight into these proxies. Table 1 Diatom Genera and Species Environment Clarification Cyclotella caspia Planktonic, marine-brackish Cosmopolitan Karayevia clevei Freshwater Cosmopolitan Melosira sp Planktonic, marine Cosmopolitan Thalassiosira sp Marine, brackish Cosmopolitan Staurosirella leptostauron Benthic, freshwater Cosmopolitan Actinoptychus senarius Planktonic or benthic, freshwater to brackish Hurricane and pluvial layers Amphora aff. sp Benthic, marine or freshwater Hurricane layers only Nitzschia sp Benthic, marine or freshwater Hurricane layers only Gomphonema sp Freshwater Hurricane layers only Surirella sp Marine-brackish Drought layer only Triceratium sp Marine Drought layer only Other Genera and species Environment Clarification

  16. Pink-color sign in esophageal squamous neoplasia, and speculation regarding the underlying mechanism

    Science.gov (United States)

    Ishihara, Ryu; Kanzaki, Hiromitsu; Iishi, Hiroyasu; Nagai, Kengo; Matsui, Fumi; Yamashina, Takeshi; Matsuura, Noriko; Ito, Takashi; Fujii, Mototsugu; Yamamoto, Sachiko; Hanaoka, Noboru; Takeuchi, Yoji; Higashino, Koji; Uedo, Noriya; Tatsuta, Masaharu; Tomita, Yasuhiko; Ishiguro, Shingo

    2013-01-01

    AIM: To investigate the reasons for the occurrence of the pink-color sign of iodine-unstained lesions. METHODS: In chromoendoscopy, the pink-color sign of iodine-unstained lesions is recognized as useful for the diagnosis of esophageal squamous cell carcinoma. Patients with superficial esophageal neoplasms treated by endoscopic resection were included in the study. Areas of mucosa with and without the pink-color sign were evaluated histologically. The following histologic features that were possibly associated with the pink-color sign were evaluated. The keratinous layer and basal cell layer were classified as present or absent. Cellular atypia was classified as high grade, moderate grade or low grade, based on nuclear irregularity, mitotic figures, loss of polarity, chromatin pattern and nuclear/cytoplasmic ratio. Vascular change was assessed based on dilatation, tortuosity, caliber change and variability in shape. Vessels with these four findings were classified as positive for vascular change. Endoscopic images of the lesions were captured immediately after iodine staining, 2-3 min after iodine staining and after complete fading of iodine staining. Quantitative analysis of color changes after iodine staining was also performed. RESULTS: A total of 61 superficial esophageal neoplasms in 54 patients were included in the study. The lesions were located in the cervical esophagus in one case, the upper thoracic esophagus in 10 cases, the mid-thoracic esophagus in 33 cases, and the lower thoracic esophagus in 17 cases. The median diameter of the lesions was 20 mm (range: 2-74 mm). Of the 61 lesions, 28 were classified as pink-color sign positive and 33 as pink-color sign negative. The histologic diagnosis was high-grade intraepithelial neoplasia (HGIN) or cancer invading into the lamina propria in 26 of the 28 pink-color sign positive lesions. There was a significant association between pink-color sign positive epithelium and HGIN or invasive cancer (P = 0

  17. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.

    Science.gov (United States)

    Gu, Xueyan; Qi, Yongmei; Feng, Zengxiu; Ma, Lin; Gao, Ke; Zhang, Yingmei

    2018-07-01

    Lead (Pb), a widely distributed environmental pollutant, is known to induce mitochondrial damage as well as autophagy in vitro and in vivo. In this study, we found that Pb could trigger mitophagy in both HEK293 cells and the kidney cortex of male Kunming mice. However, whether ataxia telangiectasis mutated (ATM) which is reported to be linked with PTEN-induced putative kinase 1 (PINK1)/Parkin pathway (a well-characterized mitophagic pathway) participates in the regulation of Pb-induced mitophagy and its exact role remains enigmatic. Our results indicated that Pb activated ATM in vitro and in vivo, and further in vitro studies showed that ATM could co-localize with PINK1 and Parkin in cytosol and interact with PINK1. Knockdown of ATM by siRNA blocked Pb-induced mitophagy even under the circumstance of enhanced accumulation of PINK1 and mitochondrial Parkin. Intriguingly, elevation instead of reduction in phosphorylation level of PINK1 and Parkin was observed in response to ATM knockdown and Pb did not contribute to the further increase of their phosphorylation level, implying that ATM indirectly regulated PINK1/Parkin pathway. These findings reveal a novel mechanism for Pb toxicity and suggest the regulatory importance of ATM in PINK1/Parkin-mediated mitophagy. Copyright © 2018 Elsevier B.V. All rights reserved.

  18. Novel P-TEN-induced putative kinase 1 (PINK1) variant in Indian Parkinson's disease patient.

    Science.gov (United States)

    Halder, Tamali; Raj, Janak; Sharma, Vivek; Das, Parimal

    2015-09-25

    Loss-of-function mutation in PINK1 is known for causing autosomal recessive early onset Parkinsonism accounting approximately 6.5% of PD cases. Recently, PINK1 has also been shown to cause Parkinson's disease (PD) in eastern India. Present study is aimed to see its contribution in north-Indian PD patients. A total of 106 PD patients and 60 ethnically matched healthy controls were included in the study. All the patients were screened for mutation in PINK1 by direct DNA sequence analysis of the PCR amplicons covering all exons and exon-intron boundaries. Identified novel variant was reconfirmed by DNA sequencing of 10 randomly selected TA clones containing the variant amplicon. In vitro functional assay of the mutant protein was performed by transfecting COS-7 cell line with wild type and mutant (created by site-directed-mutagenesis) cDNA construct of PINK1 fused to N' terminal GFP followed by western blot analysis. Two potentially pathogenic, one being novel (p.Q267X) and 6 other apparently non-pathogenic variants were identified. Western blot analysis reveals production of truncated PINK1 fusion protein of ∼55kDa in p.Q267X mutant instead of 82/93kDa of wild type PINK1 fusion protein (molecular weight of GFP is ∼27kDa). Our study concludes that PINK1 variants are prevalent for causing Parkinson's disease (PD) in India, as revealed by the occurrence of 1.8% (2/106) in PD patients from north Indian population. The novel homozygous variant of PINK1 (c.799C>T) reported here is the plausible cause for disease manifestation in this patient. Future study, however, would be helpful to understand the functional mechanism how this premature PINK1 protein (p.Q267X) responds to cellular stress leading to the PD pathophysiology. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  19. Clueless, a protein required for mitochondrial function, interacts with the PINK1-Parkin complex in Drosophila

    Directory of Open Access Journals (Sweden)

    Aditya Sen

    2015-06-01

    Full Text Available Loss of mitochondrial function often leads to neurodegeneration and is thought to be one of the underlying causes of neurodegenerative diseases such as Parkinson's disease (PD. However, the precise events linking mitochondrial dysfunction to neuronal death remain elusive. PTEN-induced putative kinase 1 (PINK1 and Parkin (Park, either of which, when mutated, are responsible for early-onset PD, mark individual mitochondria for destruction at the mitochondrial outer membrane. The specific molecular pathways that regulate signaling between the nucleus and mitochondria to sense mitochondrial dysfunction under normal physiological conditions are not well understood. Here, we show that Drosophila Clueless (Clu, a highly conserved protein required for normal mitochondrial function, can associate with Translocase of the outer membrane (TOM 20, Porin and PINK1, and is thus located at the mitochondrial outer membrane. Previously, we found that clu genetically interacts with park in Drosophila female germ cells. Here, we show that clu also genetically interacts with PINK1, and our epistasis analysis places clu downstream of PINK1 and upstream of park. In addition, Clu forms a complex with PINK1 and Park, further supporting that Clu links mitochondrial function with the PINK1-Park pathway. Lack of Clu causes PINK1 and Park to interact with each other, and clu mutants have decreased mitochondrial protein levels, suggesting that Clu can act as a negative regulator of the PINK1-Park pathway. Taken together, these results suggest that Clu directly modulates mitochondrial function, and that Clu's function contributes to the PINK1-Park pathway of mitochondrial quality control.

  20. PINK1 is required for timely cell-type specific mitochondrial clearance during Drosophila midgut metamorphosis.

    Science.gov (United States)

    Liu, Yan; Lin, Jingjing; Zhang, Minjie; Chen, Kai; Yang, Shengxi; Wang, Qun; Yang, Hongqin; Xie, Shusen; Zhou, Yongjian; Zhang, Xi; Chen, Fei; Yang, Yufeng

    2016-11-15

    Mitophagy is the selective degradation of mitochondria by autophagy, which is an important mitochondrial quality and quantity control process. During Drosophila metamorphosis, the degradation of midgut involves a large change in length and organization, which is mediated by autophagy. Here we noticed a cell-type specific mitochondrial clearance process that occurs in enterocytes (ECs), while most mitochondria remain in intestinal stem cells (ISCs) during metamorphosis. Although PINK1/PARKIN represent the canonical pathway for the elimination of impaired mitochondria in varied pathological conditions, their roles in developmental processes or normal physiological conditions have been less studied. To examine the potential contribution of PINK1 in developmental processes, we monitored the dynamic expression pattern of PINK1 in the midgut development by taking advantage of a newly CRISPR/Cas9 generated knock-in fly strain expressing PINK1-mCherry fusion protein that presumably recapitulates the endogenous expression pattern of PINK1. We disclosed a spatiotemporal correlation between the expression pattern of PINK1 and the mitochondrial clearance or persistence in ECs or ISCs respectively. By mosaic genetic analysis, we then demonstrated that PINK1 and PARKIN function epistatically to mediate the specific timely removal of mitochondria, and are involved in global autophagy in ECs during Drosophila midgut metamorphosis, with kinase-dead PINK1 exerting dominant negative effects. Taken together, our studies concluded that the PINK1/PARKIN is crucial for timely cell-type specific mitophagy under physiological conditions and demonstrated again that Drosophila midgut metamorphosis might serve as an elegant in vivo model to study autophagy. Copyright © 2016 Elsevier Inc. All rights reserved.

  1. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Shell weights of planktonic foraminifera species Globigerinoides ruber in the size range of 300–355 μm were measured from sediment traps in the western and eastern Arabian Sea which represent upwelling and non-upwelling conditions respectively. In the Western Arabian Sea Trap (WAST), G. ruber flux ranged from 33.3 ...

  2. PINK1 positively regulates IL-1β-mediated signaling through Tollip and IRAK1 modulation

    Directory of Open Access Journals (Sweden)

    Lee Hyun Jung

    2012-12-01

    Full Text Available Abstract Background Parkinson disease (PD is characterized by a slow, progressive degeneration of dopaminergic neurons in the substantianigra. The cause of neuronal loss in PD is not well understood, but several genetic loci, including PTEN-induced putative kinase 1 (PINK1, have been linked to early-onset autosomal recessive forms of familial PD. Neuroinflammation greatly contributes to PD neuronal degeneration and pathogenesis. IL-1 is one of the principal cytokines that regulates various immune and inflammatory responses via the activation of the transcription factors NF-κB and activating protein-1. Despite the close relationship between PD and neuroinflammation, the functional roles of PD-linked genes during inflammatory processes remain poorly understood. Methods To explore the functional roles of PINK1 in response to IL-1β stimulation, HEK293 cells, mouse embryonic fibroblasts derived from PINK1-null (PINK1−/− and control (PINK1+/+ mice, and 293 IL-1RI cells stably expressing type 1 IL-1 receptor were used. Immunoprecipitation and western blot analysis were performed to detect protein–protein interaction and protein ubiquitination. To confirm the effect of PINK1 on NF-κB activation, NF-κB-dependent firefly luciferase reporter assay was conducted. Results PINK1 specifically binds two components of the IL-1-mediated signaling cascade, Toll-interacting protein (Tollip and IL-1 receptor-associated kinase 1 (IRAK1. The association of PINK1 with Tollip, a negative regulator of IL-1β signaling, increases upon IL-1β stimulation, which then facilitates the dissociation of Tollip from IRAK1 as well as the assembly of the IRAK1–TNF receptor-associated factor 6 (TRAF6 complex. PINK1 also enhances Lys63-linked polyubiquitination of IRAK1, an essential modification of recruitment of NF-κB essential modulator and subsequent IκB kinase activation, and increases formation of the intermediate signalosome including IRAK1, TRAF6, and

  3. Genomic variants at the PINK1 locus are associated with transcript abundance and plasma nonesterified fatty acid concentrations in European whites

    DEFF Research Database (Denmark)

    Franks, P.W.; Scheele, C.; Loos, R.J.

    2008-01-01

    these were not statistically significant after correcting for multiple testing. In primary adipocytes, Pink1 knockdown affected fatty acid binding protein 4 (Fabp4) expression, indicating that PINK1 may influence substrate metabolism. We demonstrate that PINK1 polymorphisms are associated with PINK1...

  4. AFSC/ABL: Pink salmon data collected at Sashin Creek Weir 1934-2002

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — A database describing a 67-year time series for Sashin Creek pink salmon (Oncorhynchus gorbuscha) data is presented. The database details the survival and other...

  5. AFSC/ABL: Population structure of odd- and even-broodline Asian pink salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Electrophoretic analysis of Asian even brood-year pink salmon stocks has shown regional heterogeneity (Noll et al. in review). Hypothetical mixed fisheries were...

  6. Histological observations on the scleractinian coral Porites lutea affected by pink-line syndrome

    Digital Repository Service at National Institute of Oceanography (India)

    Ravindran, J.; Raghukumar, C.

    A pink-line syndrome (PLS) was reported in the reef building coral Porites lutea in Kavaratti island of the Lakshadweep archipelago. The affected corals had dead patches colonized by a cyanobacterium Phormidium valderianum and the bordering coral...

  7. Broodyear Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. Data is collected by broodyear on % survival to adult, % maturity as two...

  8. Production Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. Information on the number of smolts received into the program is...

  9. Fish Health Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. All fresh mortalities larger than 100 mm are sent to Fish Health for...

  10. Allium Discoloration: Color Compounds Formed during Pinking of Onion and Leek

    Czech Academy of Sciences Publication Activity Database

    Kubec, R.; Urajová, P.; Lacina, O.; Hajšlová, J.; Kuzma, Marek; Zápal, Jakub

    2015-01-01

    Roč. 63, č. 46 (2015), s. 10192-10199 ISSN 0021-8561 Institutional support: RVO:61388971 Keywords : discoloration * plant pigment * pinking Subject RIV: CE - Biochemistry Impact factor: 2.857, year: 2015

  11. Growth Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. The fork length to the nearest mm and weight to the nearest gram of a...

  12. Breeding ecology of the pink-billed lark, Spizocorys conirostris , in ...

    African Journals Online (AJOL)

    Breeding ecology of the pink-billed lark, Spizocorys conirostris , in an agricultural landscape in South Africa. ... Open Access DOWNLOAD FULL TEXT ... The results are compared with data in Birdlife South Africa's Nest Record Card Scheme.

  13. Kõigepealt on näitleja ja alles siis muu, pink kaasa arvatud / Merit Kask

    Index Scriptorium Estoniae

    Kask, Merit

    2007-01-01

    20. sept. esietendub VAT Teatris Eesti Muusika ja Teatriakadeemia Lavakunstikooli lavastajate õppesuuna tudengite koolitöö "Pink", mis koosneb kahest iseseisvast loost - Jon Fosse "Talv", lavastaja Robert Annus ja Edward Albee "Loomaaialugu", lavastaja Uku Uusberg

  14. Pink Pungi näitusel valgub veri linale / Raimu Hanson

    Index Scriptorium Estoniae

    Hanson, Raimu, 1957-

    2006-01-01

    Kunstirühmituse Pink Punk (Sandra Jõgeva, Kristin Kalamees, Aino Ingrid Sepp, Kaarel Sammet) näitusest Y-galeriis. Lähemalt videoinstallatsioonist "Must turg" ja ruumiinstallatsioonist "Elulähedane kogemus"

  15. Fish Culture Data - Captive Broodstock Gene Rescue Program for Odd Year Class Elwha River Pink Salmon

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Conduct captive brood stock gene rescue program for Elwha River odd-year class pink salmon. Raw data on rearing density, loading density, water temperature, ration,...

  16. Enhancing NAD+ salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease

    Directory of Open Access Journals (Sweden)

    Susann Lehmann

    2017-02-01

    Full Text Available Familial forms of Parkinson's disease (PD caused by mutations in PINK1 are linked to mitochondrial impairment. Defective mitochondria are also found in Drosophila models of PD with pink1 mutations. The co-enzyme nicotinamide adenine dinucleotide (NAD+ is essential for both generating energy in mitochondria and nuclear DNA repair through NAD+-consuming poly(ADP-ribose polymerases (PARPs. We found alterations in NAD+ salvage metabolism in Drosophila pink1 mutants and showed that a diet supplemented with the NAD+ precursor nicotinamide rescued mitochondrial defects and protected neurons from degeneration. Additionally, a mutation of Parp improved mitochondrial function and was neuroprotective in the pink1 mutants. We conclude that enhancing the availability of NAD+ by either the use of a diet supplemented with NAD+ precursors or the inhibition of NAD+-dependent enzymes, such as PARPs, which compete with mitochondria for NAD+, is a viable approach to preventing neurotoxicity associated with mitochondrial defects.

  17. PINK1 regulates mitochondrial trafficking in dendrites of cortical neurons through mitochondrial PKA.

    Science.gov (United States)

    Das Banerjee, Tania; Dagda, Raul Y; Dagda, Marisela; Chu, Charleen T; Rice, Monica; Vazquez-Mayorga, Emmanuel; Dagda, Ruben K

    2017-08-01

    Mitochondrial Protein Kinase A (PKA) and PTEN-induced kinase 1 (PINK1), which is linked to Parkinson's disease, are two neuroprotective serine/threonine kinases that regulate dendrite remodeling and mitochondrial function. We have previously shown that PINK1 regulates dendrite morphology by enhancing PKA activity. Here, we show the molecular mechanisms by which PINK1 and PKA in the mitochondrion interact to regulate dendrite remodeling, mitochondrial morphology, content, and trafficking in dendrites. PINK1-deficient cortical neurons exhibit impaired mitochondrial trafficking, reduced mitochondrial content, fragmented mitochondria, and a reduction in dendrite outgrowth compared to wild-type neurons. Transient expression of wild-type, but not a PKA-binding-deficient mutant of the PKA-mitochondrial scaffold dual-specificity A Kinase Anchoring Protein 1 (D-AKAP1), restores mitochondrial trafficking, morphology, and content in dendrites of PINK1-deficient cortical neurons suggesting that recruiting PKA to the mitochondrion reverses mitochondrial pathology in dendrites induced by loss of PINK1. Mechanistically, full-length and cleaved forms of PINK1 increase the binding of the regulatory subunit β of PKA (PKA/RIIβ) to D-AKAP1 to enhance the autocatalytic-mediated phosphorylation of PKA/RIIβ and PKA activity. D-AKAP1/PKA governs mitochondrial trafficking in dendrites via the Miro-2/TRAK2 complex and by increasing the phosphorylation of Miro-2. Our study identifies a new role of D-AKAP1 in regulating mitochondrial trafficking through Miro-2, and supports a model in which PINK1 and mitochondrial PKA participate in a similar neuroprotective signaling pathway to maintain dendrite connectivity. © 2017 International Society for Neurochemistry.

  18. Regional-Scale Declines in Productivity of Pink and Chum Salmon Stocks in Western North America

    Science.gov (United States)

    Malick, Michael J.; Cox, Sean P.

    2016-01-01

    Sockeye salmon (Oncorhynchus nerka) stocks throughout the southern part of their North American range have experienced declines in productivity over the past two decades. In this study, we tested the hypothesis that pink (O. gorbuscha) and chum (O. keta) salmon stocks have also experienced recent declines in productivity by investigating temporal and spatial trends in productivity of 99 wild North American pink and chum salmon stocks. We used a combination of population dynamics and time series models to quantify individual stock trends as well as common temporal trends in pink and chum salmon productivity across local, regional, and continental spatial scales. Our results indicated widespread declines in productivity of wild chum salmon stocks throughout Washington (WA) and British Columbia (BC) with 81% of stocks showing recent declines in productivity, although the exact form of the trends varied among regions. For pink salmon, the majority of stocks in WA and BC (65%) did not have strong temporal trends in productivity; however, all stocks that did have trends in productivity showed declining productivity since at least brood year 1996. We found weaker evidence of widespread declines in productivity for Alaska pink and chum salmon, with some regions and stocks showing declines in productivity (e.g., Kodiak chum salmon stocks) and others showing increases (e.g., Alaska Peninsula pink salmon stocks). We also found strong positive covariation between stock productivity series at the regional spatial scale for both pink and chum salmon, along with evidence that this regional-scale positive covariation has become stronger since the early 1990s in WA and BC. In general, our results suggest that common processes operating at the regional or multi-regional spatial scales drive productivity of pink and chum salmon stocks in western North America and that the effects of these process on productivity may change over time. PMID:26760510

  19. Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables

    OpenAIRE

    MIZUNO, Masayuki; YURIMOTO, Hiroya; YOSHIDA, Naoko; IGUCHI, Hiroyuki; SAKAI, Yasuyoshi

    2012-01-01

    The distribution of pink-pigmented facultative methylotrophs (PPFMs) on the leaves of various vegetables was studied. All kinds of vegetable leaves tested gave pink-pigmented colonies on agar plates containing methanol as sole carbon source. The numbers of PPFMs on the leaves, colony-forming units (CFU)/g of fresh leaves, differed among the plants, although they were planted and grown at the same farm. Commercial green perilla, Perilla frutescens viridis (Makino) Makino, gave the highest coun...

  20. PINK1 Primes Parkin-Mediated Ubiquitination of PARIS in Dopaminergic Neuronal Survival

    Directory of Open Access Journals (Sweden)

    Yunjong Lee

    2017-01-01

    Full Text Available Mutations in PTEN-induced putative kinase 1 (PINK1 and parkin cause autosomal-recessive Parkinson’s disease through a common pathway involving mitochondrial quality control. Parkin inactivation leads to accumulation of the parkin interacting substrate (PARIS, ZNF746 that plays an important role in dopamine cell loss through repression of proliferator-activated receptor gamma coactivator-1-alpha (PGC-1α promoter activity. Here, we show that PARIS links PINK1 and parkin in a common pathway that regulates dopaminergic neuron survival. PINK1 interacts with and phosphorylates serines 322 and 613 of PARIS to control its ubiquitination and clearance by parkin. PINK1 phosphorylation of PARIS alleviates PARIS toxicity, as well as repression of PGC-1α promoter activity. Conditional knockdown of PINK1 in adult mouse brains leads to a progressive loss of dopaminergic neurons in the substantia nigra that is dependent on PARIS. Altogether, these results uncover a function of PINK1 to direct parkin-PARIS-regulated PGC-1α expression and dopaminergic neuronal survival.

  1. Red, Purple and Pink: The Colors of Diffusion on Pinterest

    Science.gov (United States)

    Bakhshi, Saeideh; Gilbert, Eric

    2015-01-01

    Many lab studies have shown that colors can evoke powerful emotions and impact human behavior. Might these phenomena drive how we act online? A key research challenge for image-sharing communities is uncovering the mechanisms by which content spreads through the community. In this paper, we investigate whether there is link between color and diffusion. Drawing on a corpus of one million images crawled from Pinterest, we find that color significantly impacts the diffusion of images and adoption of content on image sharing communities such as Pinterest, even after partially controlling for network structure and activity. Specifically, Red, Purple and pink seem to promote diffusion, while Green, Blue, Black and Yellow suppress it. To our knowledge, our study is the first to investigate how colors relate to online user behavior. In addition to contributing to the research conversation surrounding diffusion, these findings suggest future work using sophisticated computer vision techniques. We conclude with a discussion on the theoretical, practical and design implications suggested by this work—e.g. design of engaging image filters. PMID:25658423

  2. Bioaccumulation of animal adenoviruses in the pink shrimp

    Directory of Open Access Journals (Sweden)

    Roger B. Luz

    2015-09-01

    Full Text Available Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100 Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems.

  3. Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit colour

    NARCIS (Netherlands)

    Ballester, A.R.; Molthoff, J.W.; Vos, de C.H.; Lintel Hekkert, B.; Orzaez, D.; Fernandez-Moreno, J.P.; Tripodi, S.; Grandillo, S.; Martin, C.; Heldens, J.; Ykema, M.; Granell, A.; Bovy, A.G.

    2010-01-01

    The color of tomato fruit is mainly determined by carotenoids and flavonoids. Phenotypic analysis of an introgression line (IL) population derived from a cross between Solanum lycopersicum 'Moneyberg' and the wild species Solanum chmielewskii revealed three ILs with a pink fruit color. These lines

  4. Migratory routes and at-sea threats to Pink-footed Shearwaters

    Science.gov (United States)

    Adams, Josh; Felis, Jonathan J.; Hodum, Peter; Colodro, Valentina; Carle, Ryan; López, Verónica

    2016-01-01

    The Pink-footed Shearwater (Ardenna creatopus) is a seabird with a breeding range restricted to three islands in Chile and an estimated world population of approximately 56,000 breeding individuals (Muñoz 2011, Oikonos unpublished data). Due to multiple threats on breeding colonies and at-sea, Pink-footed Shearwaters are listed as Endangered by the government of Chile (Reglamento de Clasificación de Especies, 2011), Threatened by the government of Canada (Environment Canada 2008), and are listed under Appendix 1 of the Agreement on the Conservation of Albatrosses and Petrels (ACAP 2013). A principal conservation concern for the species is mortality from fisheries bycatch during the breeding and non-breeding seasons; thus, identification of areas of overlap between at-sea use by Pink-footed Shearwaters and fisheries is a high priority conservation objective (Hinojosa Sáez and Hodum 1997, Mangel et al. 2013, ACAP 2013). During the non-breeding period, Pink-footed Shearwaters range as far north as Canada, although little was known until recently about migration routes and important wintering areas where fisheries bycatch could be a risk. Additionally, Pink-footed Shearwaters face at-sea threats during the non-breeding season off the west coast of North America. Recently, areas used by wintering Pink-footed Shearwaters have been identified as areas of interest for developing alternative energy offshore in North America (e.g., floating wind generators; Trident Winds 2016). The goal of our study was to track Pink-footed Shearwater post-breeding movements with satellite tags to identify timing and routes of migration, locate important non-breeding foraging habitats, and determine population distribution among different wintering regions.

  5. Antiplatelet activity of white and pink Nelumbo nucifera Gaertn flowers

    Directory of Open Access Journals (Sweden)

    Brindha Durairaj

    2010-09-01

    Full Text Available Nelumbo nucifera Gaertn (Nelumbonaceae a plant used in Ayurvedic medicine (common name: lotus, is a perennial, large and rhizomatous aquatic herb most prevalent in South India. Preliminary phytochemical screening of both white and pink Nelumbo nucifera flowers revealed the presence of phytochemical constituents (flavonoids, alkaloids, phenols etc,. Hence, an attempt has been made to screen the effect of Nelumbo nucifera flowers (both types on platelet aggregation. The antiplatelet activity of hydroethanolic extract of both types of flowers was studied using platelet-rich plasma in different concentrations (100-500µg/ml. Both white and pink Nelumbo nucifera flower extracts showed dose-dependent effective antiplatelet activity with maximum activity at 500µg/ml concentration; prevention of platelet aggregation was 50% of that achieved with standard aspirin. Furthermore, the antiplatelet activity of white flowers was relatively high (pNelumbo nucifera Gaertn (Nelumbonaceae, planta utilizada na medicina Ayurvédica, é erva aquática rizomatosa grande, predominante no sul da Índia. A triagem fitoquímica preliminar das flores brancas e cor-de-rosa de Nelumbo nucifera revelou a presença de constituintes fitoquímicos (flavonoides, alcaloides, fenóis etc. Assim, tentou-se a triagem do efeito das flores de Nelumbo nucifera de ambos os tipos na agregação plaquetária. A atividade antiplaquetária dos extratos hidroetanólico de ambos os tipos de flores foi estudada, utilizando-se plasma rico em plaquetas em duas diferentes concentrações (100 - 500 µg/mL. Tanto os extratos das flores brancas quanto daquelas de cor-de-rosa mostraram atividade antiplaquetária dose-dependente, com o máximo na concentração de 500 µg/mL. A prevenção da agregação plaquetária foi 50% daquela alcançada com o padrão de ácido acetilsalicílico. Além disso, a atividade antiplaquetária das flores brancas foi, relativamente, alta (p<0,05; ANOVA

  6. The PINK1-Parkin pathway is involved in the regulation of mitochondrial remodeling process

    International Nuclear Information System (INIS)

    Park, Jeehye; Lee, Gina; Chung, Jongkyeong

    2009-01-01

    The two Parkinson's disease (PD) genes, PTEN-induced kinase 1 (PINK1) and parkin, are linked in a common pathway which affects mitochondrial integrity and function. However, it is still not known what this pathway does in the mitochondria. Therefore, we investigated its physiological function in Drosophila. Because Drosophila PINK1 and parkin mutants show changes in mitochondrial morphology in both indirect flight muscles and dopaminergic neurons, we here investigated whether the PINK1-Parkin pathway genetically interacts with the regulators of mitochondrial fusion and fission such as Drp1, which promotes mitochondrial fission, and Opa1 or Marf, which induces mitochondrial fusion. Surprisingly, DrosophilaPINK1 and parkin mutant phenotypes were markedly suppressed by overexpression of Drp1 or downregulation of Opa1 or Marf, indicating that the PINK1-Parkin pathway regulates mitochondrial remodeling process in the direction of promoting mitochondrial fission. Therefore, we strongly suggest that mitochondrial fusion and fission process could be a prominent therapeutic target for the treatment of PD.

  7. Early hypersynchrony in juvenile PINK1-/- motor cortex is rescued by antidromic stimulation

    Directory of Open Access Journals (Sweden)

    Romain eCARRON

    2014-05-01

    Full Text Available In Parkinson’s disease, cortical networks show enhanced synchronized activity but whether this precedes motor signs is unknown. We investigated this question in PINK1-/- mice, a genetic rodent model of the PARK6 variant of familial Parkinson’s disease which shows impaired spontaneous locomotion at 16 months. We used two-photon calcium imaging and whole-cell patch clamp in slices from juvenile (P14-P21 wild-type or PINK1-/- mice. We designed a horizontal tilted cortico-subthalamic slice where the only connection between cortex and subthalamic nucleus (STN is the hyperdirect cortico-subthalamic pathway. We report excessive correlation and synchronization in PINK1-/- M1 cortical networks 15 months before motor impairment. The percentage of correlated pairs of neurons and their strength of correlation were higher in the PINK1-/- M1 than in the wild type network and the synchronized network events involved a higher percentage of neurons. Both features were independent of thalamo-cortical pathways, insensitive to chronic levodopa treatment of pups, but totally reversed by antidromic invasion of M1 pyramidal neurons by axonal spikes evoked by high frequency stimulation (HFS of the STN. Our study describes an early excess of synchronization in the PINK1-/- cortex and suggests a potential role of antidromic activation of cortical interneurons in network desynchronization. Such backward effect on interneurons activity may be of importance for HFS-induced network desynchronization.

  8. Empirical evaluation of cell critical volume dose vs. cell response function for pink mutations in tradescantia

    International Nuclear Information System (INIS)

    Varma, M.N.; Bond, V.P.

    1982-01-01

    Microdosimetric spectra for 0.43, 1.8, and 14.7 MeV neutrons, and for 215 kVp x rays and 1250 keV gammas were used in conjunction with relative biological effectiveness (RBE) values for pink mutations in Tradescantia to obtain an effectiveness function (i.e., a cell critical volume dose vs. cell response function). This effectiveness function (or hit size weighting function) provides the probability of inducing a biological effect of interest (in the present study, pink mutations in Tradescantia) as a function of lineal energy density y. In a preliminary analysis the critical value of y above which pink mutations are seen was 4.5 keV/μm, and the value of y at which the probability reaches unity was 115 keV/μm. Idealized but approximate event size distributions for mono-LET particles ranging from 10 to 5000 keV/μm were generated, and these distributions were weighted by the effectiveness function to determine the pink mutation frequencies. Results are compared with measured pink mutation frequencies for 11 keV/μm ( 12 C) and 31 keV/μm ( 20 Ne) ions

  9. Supplementing long-chain n-3 polyunsaturated fatty acids in canned wild Pacific pink salmon with Alaska salmon oil

    Science.gov (United States)

    Lapis, Trina J; Oliveira, Alexandra C M; Crapo, Charles A; Himelbloom, Brian; Bechtel, Peter J; Long, Kristy A

    2013-01-01

    Establishing n-3 polyunsaturated fatty acid contents in canned wild Alaska pink salmon products is challenging due to ample natural variation found in lipid content of pink salmon muscle. This study investigated the effect of adding salmon oil (SO) to canned pink salmon produced from fish exhibiting two opposite degrees of skin watermarking, bright (B) and dark (D). Specific goals of the study were to evaluate the benefits of adding SO to canned pink salmon with regard to nutritional value of the product, sensory characteristics, and the oxidative and hydrolytic stability of the lipids over thermal processing. Six groups of canned pink salmon were produced with variable levels of SO, either using bright (with 0, 1, or 2% SO) or dark (with 0, 2, or 4% SO) pink salmon. Compositional analysis revealed highest (P  0.05) ranging from 5.7% to 6.8%. Consequently, addition of SO to canned pink salmon allowed for consistent lipid content between bright and dark fish. Addition of 1% or 2% SO to canned bright pink salmon was not detrimental to the sensory properties of the product. It is recommended that canned bright pink salmon be supplemented with at least 1% SO, while supplementation with 2% SO would guarantee a minimum quantity of 1.9 g of n-3 fatty acids per 100 g of product. Addition of 4% SO to canned dark pink salmon was detrimental to product texture and taste, while supplementation with 2% SO did not negatively affect sensorial properties of the product. Accordingly, canned dark pink salmon should be supplemented with 2% SO so that a minimum n-3 fatty acids content of 1.5 g per 100 g of product. PMID:24804010

  10. Parallel signatures of selection in temporally isolated lineages of pink salmon

    DEFF Research Database (Denmark)

    Seeb, L. W.; Waples, R. K.; Limborg, M. T.

    2014-01-01

    Studying the effect of similar environments on diverse genetic backgrounds has long been a goal of evolutionary biologists with studies typically relying on experimental approaches. Pink salmon, a highly abundant and widely ranging salmonid, provide a naturally occurring opportunity to study......-associated DNA (RAD) sequencing to discover and genotype approximately 8000 SNP loci in three population pairs of even- and odd-year pink salmon along a latitudinal gradient in North America. We found greater differentiation within the odd-year than within the even-year lineage and greater differentiation...... be particularly informative in understanding adaptive evolution in pink salmon and exploring how differing genetic backgrounds within a species respond to selection from the same natural environment...

  11. Induction of in vivo mutation in chrysanthemum (Dendranthema grandiflora Tzvelev) cv. Pink Repin breeding

    International Nuclear Information System (INIS)

    Neto, Augusto Tulmann; Latado, Rodrigo Rocha

    1997-01-01

    Mutation induction was used with the objective of obtaining mutants for flower colour of chrysanthemum, cv. Repin (pink colour). Rooted cuttings were irradiated with 20 Gy of gamma rays and before the selection the cutting back method was used to advance the generations. The frequency of colour mutants observed was 5.8%. Among the mutants obtainedthe white and dark-pink-coloured ones were evaluated in yield trial and post-harvest. The results indicated that these mutants mantained the same agronomical characteristics showed by the control, with the exception of plant height in the white mutant that was shorter. Due to commercial interest of the producers, these mutants were multiplied and released as new cultivars. The white flower colour mutant was named Cristiane and the dark-pink, Ingrid. This was the first example of cultivars from an ornamental plant released by mutation breeding in Brazil. (author)

  12. The effect of irradiation in the preservation of pink pepper (Schinus terebinthifolius Raddi)

    Science.gov (United States)

    de Souza, Adriana Régia Marques; Arthur, Valter; Nogueira, Danielle Pires

    2012-08-01

    Pink peppers, also known as "pimenta-rosa" and "poivre rose", are the fruit of Schinus terebinthifolius Raddi, a species of pepper cultivated in Brazil, and have great potential for the exploration of uses. In efforts to lengthen the shelf life of this pepper, the purpose of this study was to evaluate the effect of different doses of radiation on its physical composition and color. The pink pepper samples were irradiated with doses of 0, 0.2, 0.4, 0.8 and 1.6 kGy, and the moisture, ash and lipid contents, pH and color were analyzed. The moisture content, lipid content and pH analysis indicated effects due to the irradiation (p>0.05) in which the higher doses resulted in decreases in the attribute. In contrast, there were no significant differences for the ash analysis (ppreservation process for pink peppers.

  13. EFTIHIYA – THE NEWEST WINE AROMATIC VARIETY WITH PINK COLORED BERRY

    OpenAIRE

    Zamanidi P. C.; Troshin L. P.; Radchevskiy P. P.

    2014-01-01

    The newest wine grape variety Eftihiya with aromatic pink colored skin (in Greek the name means “happi-ness”) was breed with hybridization at the Athens In-stitute of Viticulture by P. Zamanidi, L. Troshin and P. Radchevskiy in 2005 by crossing the Greek varieties and Malaguzya with Eurasian Traminer pink. Dura-tion of production period from bud burst to harvest 146-155 days. Yields are very high: 25-30 t / ha. Modal mass of clusters is 250 g. Bunch is conical, me-dium density. Berry is mediu...

  14. PINK TOMATO CETUS F1 – PROSPECTS OF THE RUSSIAN MARKET

    Directory of Open Access Journals (Sweden)

    A. Yu. Barbaritskiy

    2017-01-01

    Full Text Available Pink tomato due to their high taste quality is particularly important crop for consumers in Russia. Pink tomato seeds become more popular in the market every year. Breeders have accomplished to combine high fruit taste quality with good transportation abilities and fruit denseness. One of the latest breeding achievement is Cetus F1. A successful combination of good fruit taste with transportability and yield makes this variety a potential leader in Russian market. This is confirmed by growers' trials in various regions of South of Russia. 

  15. I-ST INTERNATIONAL CONFERENCE. "PINK TOMATO. PRODUCTION. CONSUMPTION. MARKET TRENDS IN CENTRAL ASIA».

    Directory of Open Access Journals (Sweden)

    Sh. Alimov

    2015-01-01

    Full Text Available 28 May 2015, in Tashkent, Uzbekistan, the Ist International conference "Pink tomato. Production, consumption, market trends" was held. The problems of breedong, seed production of cultivars and hybrids of pink tomato, as well as increasing of consumer's demand have been considered. The conference has provided good opportunity for meetings of producers, breeders, and researchers from different countries.

  16. Efficacy of pink guava pulp as an antioxidant in raw pork emulsion.

    Science.gov (United States)

    Joseph, Serlene; Chatli, Manish K; Biswas, Ashim K; Sahoo, Jhari

    2014-08-01

    Lipid oxidation-induced quality problems can be minimized with the use of natural antioxidants. The antioxidant potential of pink guava pulp (PGP) was evaluated at different levels (0%; C, 5.0%; T-1, 7.5%; T-2 and 10.0%; T-3) in the raw pork emulsion during refrigerated storage of 9 days under aerobic packaging. Lycopene and β-carotene contents increased (P emulsion than control throughout storage period. Our results indicated that pink guava pulp can be utilized as antioxidants in raw pork products to minimize lipid oxidation, off-odour development, and surface discolouration.

  17. ‘De ongelukkigsten onder de menschen’ De Verbeelding van zenuwzwakte in advertenties voor Pink pillen, 1900-1920

    NARCIS (Netherlands)

    Blok, Gemma; Spijkerman, Rose

    2013-01-01

    abstractBetween 1880 and 1920 Pink Pills, a medication for blood and nerve strengthening, were heavily advertised in Dutch newspapers. The advertisements consisted of the personal stories of patients who had been amazingly healed. This article argues that the Pink Pill advertisements contributed to

  18. Parámetros de crecimiento y mortalidad de Orthopristis ruber (Perciformes: Haemulidae en el archipiélago Los Frailes, Venezuela

    Directory of Open Access Journals (Sweden)

    Atenas Guerrieri

    2015-03-01

    Full Text Available Orthopristis ruber es una especie de gran demanda en el oriente de Venezuela cuya producción se ha visto disminuida en los últimos años. Por esta razón, se planteó como objetivo estimar los parámetros de crecimiento y mortalidad del recurso. Se realizaron muestreos mensuales de junio 2011 a mayo 2012, para un total de 2 980 ejemplares recolectados en El Tirano y Puerto Abajo. A cada ejemplar se le tomó datos de longitud total (cm, peso total (g y se determinó el sexo. Se comprobó que no existe diferencia sexual con respecto a longitud (t s=-1.113; p>0.05, la relación longitud-peso para ambos sexos fue: Pt=0.0612*Lt2.54, lo que evidencia un crecimiento alométrico minorante. La estimación del crecimiento se basó en el análisis de la distribución de frecuencia de longitudes, con uso del software FiSAT. Los parámetros de crecimiento estimados (L∞=39.03cm, P∞=679.60g, k=0.48/año y t o=-0.32año mostraron un crecimiento moderadamente rápido. Los datos de frecuencias de longitudes fueron ajustados al modelo de von Bertalanffy, e indica una tendencia de tipo exponencial con crecimiento acelerado los primeros años de edad, que luego se hizo lento hasta que el pez alcanzó la longitud máxima. El coeficiente de variación del índice de crecimiento (Ø’ demostró que no hay diferencias en el patrón de crecimiento. La tasa de mortalidad natural (M=0.97/año, por pesca (F=1.57/año, y total (Z=2.54/año fueron altas así como la tasa de explotación (E=0.62/año. Se concluye que O. ruber está plenamente explotado por la pesca artesanal; por lo que se sugiere continuar con los estudios de dinámica de poblaciones para evaluar la pesquería con fines de manejo.

  19. Efeito dos resíduos de café seco e fermentado por Monascus ruber no metabolismo de camundongos Apo E

    Directory of Open Access Journals (Sweden)

    Larissa Froede Brito

    2012-08-01

    Full Text Available FUNDAMENTO: A aterosclerose é uma doença inflamatória crônica de origem multifatorial que ocorre em resposta à agressão endotelial. O fungo Monascus ruber apresenta atividade hipocolesterolêmica e polifenóis presentes no resíduo de café apresentam atividade antioxidante, podendo auxiliar na prevenção de doenças cardiovasculares. O resíduo de café possui quantidade significativa de açúcares fermentescíveis, constituindo-se em substrato apropriado para o cultivo de fungos. OBJETIVO: O objetivo deste estudo foi avaliar o efeito dos resíduos de café seco e fermentado por Monascus ruber no metabolismo lipídico de camundongos knockout Apo E. MÉTODOS: O ensaio biológico foi realizado com 30 camundongos knockout para o gene Apo E, divididos em cinco grupos e submetidos a diferentes tratamentos. Foi realizada a prospecção fitoquímica e quantificação de compostos fenólicos dos resíduos fermentado e sem fermentar. O soro dos animais foi analisado utilizando kits enzimáticos e o tecido aórtico incluso em parafina e corado com H/E para realização da análise histopatológica. RESULTADOS: O resíduo de café sem fermentar 2%, em relação ao grupo controle, diminuiu em 42% o nível sérico de triacilgliceróis e em aproximadamente 41% a fração VLDL-c. Os grupos dos animais alimentados com 10% de resíduo não fermentado e 2% de resíduo fermentado diminuíram a área de lesão 10,5% e 15,4%, respectivamente, quando comparados ao controle. O resíduo fermentado apresentou um teor de compostos fenólicos superior ao resíduo não fermentado. CONCLUSÃO: O presente estudo mostra que a fermentação do resíduo de café apresenta potencial efeito benéfico sobre as doenças cardiovasculares, especialmente a aterosclerose.

  20. PINK1-Parkin alleviates metabolic stress induced by obesity in adipose tissue and in 3T3-L1 preadipocytes.

    Science.gov (United States)

    Cui, Chen; Chen, Shihong; Qiao, Jingting; Qing, Li; Wang, Lingshu; He, Tianyi; Wang, Chuan; Liu, Fuqiang; Gong, Lei; Chen, Li; Hou, Xinguo

    2018-04-06

    Mitochondria play an important role in cellular metabolism and are closely related with metabolic stress. Recently, several studies have shown that mitophagy mediated by PTEN-induced putative kinase 1 (PINK1) and Parkin may play a critical role in clearing the damaged mitochondria and maintaining the overall balance of intracellular mitochondria in quality and quantity. A previous study showed that PINK1 and Parkin were overexpressed in adipose tissue in obese subjects. However, it is still unclear whether a direct relationship exists between obesity and mitophagy. In this study, we created a high-fat-diet (HFD)-induced obese mouse model and examined the expression of PINK1 and Parkin in adipose tissue using western blot and real-time quantitative PCR. After we confirmed that there is an interesting difference between regular-chow-fed mice and HFD-induced obese mice in the expression of PINK1 and Parkin in vivo, we further tested the expression of PINK1 and Parkin in 3T3-L1 preadipocytes in vitro by treating cells with palmitic acid (PA) to induce metabolic stress. To better understand the role of PINK1 and Parkin in metabolic stress, 3T3-L1 preadipocytes were transfected with small interfering RNA (siRNA) of PINK1 and Parkin followed by PA treatment. Our results showed that under lower concentrations of PA, PINK1 and Parkin can be activated and play a protective role in resisting the harmful effects of PA, including protecting the mitochondrial function and resisting cellular death, while under higher concentrations of PA, the expression of PINK1 and Parkin can be inhibited. These results suggest that PINK1-Parkin can protect mitochondrial function against metabolic stress induced by obesity or PA to a certain degree. Copyright © 2018 Elsevier Inc. All rights reserved.

  1. Low Latitude Pelagic Foraminifera Found in the Hudson River: Are They Hurricane Deposits?

    Science.gov (United States)

    Monahan, K. M.; Abbott, D. H.; Hoenisch, B.; Breger, D.

    2011-12-01

    River sediment cores provide a record of past environmental changes through stacked layers of sediments. In core CD02-29A, recovered from the southern Hudson River, a significant number of tropical planktic foraminifer tests were found. Foraminifera were concentrated in sediment layers of low impedance, suggesting high carbonate content. Because modern planktic foraminifera are exclusively marine, their presence in Hudson sediments in the core was remarkable. We can think of only two mechanisms that could explain this observation: either living specimens are carried upriver with the daily tides, or storm surges carry large amounts of seawater and re-suspended marine sediment upriver. To test for the presence of living specimens in Hudson River water, plankton tow samples were collected during high tide at the Hudson Battery south of the sample site, and at Piermont Pier north of the sample site and no living foraminifera were found. In addition, oxygen isotope (δ18O) analyses reveal a marine composition but the large difference in δ18O between the two surface dwelling species Globigerinoides ruber (pink) and Globigerinoides sacculifer, picked from the same sediment layer, suggests re-suspension and mixing of marine sediment deposits. Because only planktic, tropical to subtropical foraminiferal assemblages were found, the Hudson River deposits differ from previously recorded storm deposits found on Long Island and in New Jersey. In particular, the foraminiferal assemblages contain up to 40% G. ruber (pink), suggesting a highly tropical signal from a location where abundances of G. ruber are very low. This data, in addition to the pulsed occurrence of tests in the sediment suggests that the introduction of planktic foraminifera into the Hudson River must be driven by rare events. We suggest that storm surges from rare high-intensity hurricanes most likely explain the presence of these tests in Hudson River sediments, possibly assisted by the Gulf Stream entraining

  2. Geomagnetic imprinting predicts spatio-temporal variation in homing migration of pink and sockeye salmon.

    Science.gov (United States)

    Putman, Nathan F; Jenkins, Erica S; Michielsens, Catherine G J; Noakes, David L G

    2014-10-06

    Animals navigate using a variety of sensory cues, but how each is weighted during different phases of movement (e.g. dispersal, foraging, homing) is controversial. Here, we examine the geomagnetic and olfactory imprinting hypotheses of natal homing with datasets that recorded variation in the migratory routes of sockeye (Oncorhynchus nerka) and pink (Oncorhynchus gorbuscha) salmon returning from the Pacific Ocean to the Fraser River, British Columbia. Drift of the magnetic field (i.e. geomagnetic imprinting) uniquely accounted for 23.2% and 44.0% of the variation in migration routes for sockeye and pink salmon, respectively. Ocean circulation (i.e. olfactory imprinting) predicted 6.1% and 0.1% of the variation in sockeye and pink migration routes, respectively. Sea surface temperature (a variable influencing salmon distribution but not navigation, directly) accounted for 13.0% of the variation in sockeye migration but was unrelated to pink migration. These findings suggest that geomagnetic navigation plays an important role in long-distance homing in salmon and that consideration of navigation mechanisms can aid in the management of migratory fishes by better predicting movement patterns. Finally, given the diversity of animals that use the Earth's magnetic field for navigation, geomagnetic drift may provide a unifying explanation for spatio-temporal variation in the movement patterns of many species. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

  3. The Coming Right-Brain Economy: Daniel H. Pink Says the MFA Is the New MBA

    Science.gov (United States)

    Connection: The Journal of the New England Board of Higher Education, 2005

    2005-01-01

    According to a recent "Harvard Business Review" piece by author Daniel H. Pink, MBA graduates are, in many ways, becoming this century's blue-collar workers--people who entered a workforce that was full of promise only to see their jobs move overseas. At the same time, businesses are realizing that the only way to differentiate their goods and…

  4. The mixed Ax-Lindemann theorem and its applications to the Zilber-Pink conjecture

    NARCIS (Netherlands)

    Gao, Ziyang

    2014-01-01

    The Zilber-Pink conjecture is a common generalization of the Andre-Oort and the Mordell-Lang conjectures. In this dissertation, we study its sub-conjectures: Andre-Oort, which predicts that a subvariety of a mixed Shimura variety having dense intersection with the set of special points is special;

  5. Demography and monitoring of Welsh's milkweed (Asclepias welshii) at Coral Pink Sand Dunes

    Science.gov (United States)

    Brent C. Palmer; L. Armstrong

    2001-01-01

    Results are presented of a 12-year monitoring program on the Coral Pink Sand Dunes and Sand Hills populations of the threatened Welsh's milkweed, Asclepias welshii N & P Holmgren. The species is an early sera1 member of the dune flora, colonizing blowouts and advancing with shifting dunes. When an area stabilizes and other vegetation encroaches, A. welshii is...

  6. Elegant lady a pink longiflorum lily cultivar suitable for cut flower forcing

    NARCIS (Netherlands)

    Lim Ki-Byung,; Tuyl, van J.M.

    2004-01-01

    A new pink longiflorum (Lilium longiflorum x (L. longiforum x L. rubellum)) lily cultivar was obtained by integrated intersectional hybridization techniques. The F1 intersectional hybrid was obtained by crossing between L. longiflorum 'Gelria' and wild type L. rubellum. Since the F1 hybrid was

  7. Geomagnetic imprinting predicts spatio-temporal variation in homing migration of pink and sockeye salmon

    Science.gov (United States)

    Putman, Nathan F.; Jenkins, Erica S.; Michielsens, Catherine G. J.; Noakes, David L. G.

    2014-01-01

    Animals navigate using a variety of sensory cues, but how each is weighted during different phases of movement (e.g. dispersal, foraging, homing) is controversial. Here, we examine the geomagnetic and olfactory imprinting hypotheses of natal homing with datasets that recorded variation in the migratory routes of sockeye (Oncorhynchus nerka) and pink (Oncorhynchus gorbuscha) salmon returning from the Pacific Ocean to the Fraser River, British Columbia. Drift of the magnetic field (i.e. geomagnetic imprinting) uniquely accounted for 23.2% and 44.0% of the variation in migration routes for sockeye and pink salmon, respectively. Ocean circulation (i.e. olfactory imprinting) predicted 6.1% and 0.1% of the variation in sockeye and pink migration routes, respectively. Sea surface temperature (a variable influencing salmon distribution but not navigation, directly) accounted for 13.0% of the variation in sockeye migration but was unrelated to pink migration. These findings suggest that geomagnetic navigation plays an important role in long-distance homing in salmon and that consideration of navigation mechanisms can aid in the management of migratory fishes by better predicting movement patterns. Finally, given the diversity of animals that use the Earth's magnetic field for navigation, geomagnetic drift may provide a unifying explanation for spatio-temporal variation in the movement patterns of many species. PMID:25056214

  8. Superoxide dismutating molecules rescue the toxic effects of PINK1 and parkin loss.

    Science.gov (United States)

    Biosa, Alice; Sanchez-Martinez, Alvaro; Filograna, Roberta; Terriente-Felix, Ana; Alam, Sarah M; Beltramini, Mariano; Bubacco, Luigi; Bisaglia, Marco; Whitworth, Alexander J

    2018-05-01

    Reactive oxygen species exert important functions in regulating several cellular signalling pathways. However, an excessive accumulation of reactive oxygen species can perturb the redox homeostasis leading to oxidative stress, a condition which has been associated to many neurodegenerative disorders. Accordingly, alterations in the redox state of cells and mitochondrial homeostasis are established hallmarks in both familial and sporadic Parkinson's disease cases. PINK1 and Parkin are two genes which account for a large fraction of autosomal recessive early-onset forms of Parkinson's disease and are now firmly associated to both mitochondria and redox homeostasis. In this study we explored the hypothesis that superoxide anions participate in the generation of the Parkin and PINK1 associated phenotypic effect by testing the capacity of endogenous and exogenous superoxide dismutating molecules to rescue the toxic effects induced by loss of PINK1 or Parkin, in both cellular and fly models. Our results demonstrate the positive effect of an increased level of superoxide dismutase proteins on the pathological phenotypes, both in vitro and in vivo. A more pronounced effectiveness for mitochondrial SOD2 activity points to the superoxide radicals generated in the mitochondrial matrix as the prime suspect in the definition of the observed phenotypes. Moreover, we also demonstrate the efficacy of a SOD-mimetic compound, M40403, to partially ameliorate PINK1/Parkin phenotypes in vitro and in vivo. These results support the further exploration of SOD-mimetic compounds as a therapeutic strategy against Parkinson's disease.

  9. Complete Genome Sequence of Methylobacterium populi P-1M, Isolated from Pink-Pigmented Household Biofilm

    OpenAIRE

    Morohoshi, Tomohiro; Ikeda, Tsukasa

    2016-01-01

    Methylobacterium populi P-1M is isolated from the pink-pigmented household biofilm. Here, we present the complete genome sequence of P-1M, consisting of one chromosome of 5,705,640?bp and five plasmids of 64,864?bp, 59,879?bp, 42,569?bp, 41,417?bp, and 29,506?bp.

  10. Genetic and metabolic diversity of pink-pigmented facultative methylotrophs in phyllosphere of tropical plants

    OpenAIRE

    Balachandar, D.; Raja, P.; Sundaram, SP.

    2008-01-01

    Diversity of Pink-Pigmented Facultative Methylotrophs (PPFMs) in phyllosphere of cotton, maize and sunflower was determined based on differential carbon-substrate utilization profile and Random Amplified Polymorphic DNA data. Results indicate that six diversified groups of PPFMs are found in these crops. Sunflower and maize phyllosphere harbor four different groups of methylobacteria while cotton has only two groups.

  11. Genetic and metabolic diversity of pink-pigmented facultative methylotrophs in phyllosphere of tropical plants.

    Science.gov (United States)

    Balachandar, D; Raja, P; Sundaram, Sp

    2008-01-01

    Diversity of Pink-Pigmented Facultative Methylotrophs (PPFMs) in phyllosphere of cotton, maize and sunflower was determined based on differential carbon-substrate utilization profile and Random Amplified Polymorphic DNA data. Results indicate that six diversified groups of PPFMs are found in these crops. Sunflower and maize phyllosphere harbor four different groups of methylobacteria while cotton has only two groups.

  12. Pop nädal : Pink muutis taas imagot. Billboard 200 TOP 20 / Karolina Põder

    Index Scriptorium Estoniae

    Põder, Karolina

    2002-01-01

    Ameerika popstaarist Pink (Alecia Moorist). Crazy Town'i uuest albumist "Darkhorse". Hard Rock Club' üritusest Biker's Pubis Tallinnas 5. okt.. Impaled Nazarene HR STAR üritusel Nõmme kultuurimajas 25. okt.. Saksa popkuulsuse Alphaville saabumisest Riiga Krasdtmala festivalile 4. oktoobril

  13. A comparative study of LRRK2, PINK1 and genetically undefined familial Parkinson's disease.

    Science.gov (United States)

    Nishioka, Kenya; Kefi, Mounir; Jasinska-Myga, Barbara; Wider, Christian; Vilariño-Güell, Carles; Ross, Owen A; Heckman, Michael G; Middleton, Lefkos T; Ishihara-Paul, Lianna; Gibson, Rachel A; Amouri, Rim; Ben Yahmed, Samia; Ben Sassi, Samia; Zouari, Mourad; El Euch, Ghada; Farrer, Matthew J; Hentati, Faycal

    2010-04-01

    Genetic classification of Parkinson's disease (PD) subtypes may become the preferred diagnostic tool for neurologists. Herein we compare clinical features from a large cohort of patients with familial PD of unknown aetiology or attributable to distinct genetic forms. Comprehensive neurological examinations were performed in 231 familial PD patients from Tunisia. Analysis was previously performed to screen for mutations in leucine rich repeat kinase 2 (LRRK2), PTEN induced kinase 1 (PINK1) and parkin (PRKN). Clinical features were compared between patients with genetically undefined PD (n=107) and those with LRRK2 (n=73) and PINK1 (n=42) mutations using regression analyses adjusted for gender, age of onset and disease duration. PRKN cases (n=9) were too few for meaningful statistical analysis. In comparison with genetically undefined patients, LRRK2 mutation carriers had more severe motor symptoms (median Unified Parkinson's Disease Rating Scale scores approximately 1.6 times higher, pundefined patients. As expected, PINK1 patients had younger ages and ages at disease onset, and a longer disease duration compared with LRRK2 mutation carriers and genetically undefined patients. Clinical differences between LRRK2, PINK1 and genetically undefined familial PD appear more pronounced than previously appreciated, and may prove useful in clinical practice. As future therapies are targeted to specific protein abnormalities, identifying the genetic causes and associated clinical and pathological features will determine diagnosis, preventative medicine and drug intervention strategies.

  14. Why girls go pink: Game character identification and game-players’ motivations

    NARCIS (Netherlands)

    van Reijmersdal, Eva A.; Jansz, Jeroen; Peters, O.; van Noort, Guda

    2013-01-01

    Based on social role theory and uses and gratifications, this study provides insights into the popularity of so-called pink games. This study is the first to examine the roles of identification, playing time, and age in the experience of motivations while playing an online role-playing game. Drawing

  15. Why girls go pink: game character identification and game-players’ motivations

    NARCIS (Netherlands)

    van Reijmersdal, E.A.; Jansz, J.; Peters, O.; van Noort, G.

    2013-01-01

    Based on social role theory and uses and gratifications, this study provides insights into the popularity of so-called pink games. This study is the first to examine the roles of identification, playing time, and age in the experience of motivations while playing an online role-playing game. Drawing

  16. Pink Tea to Proactive: CCET's Transition Years, 1961-1982, as I Remember Them

    Science.gov (United States)

    Thompson, Alvin H.

    2015-01-01

    Alvin Thompson, former president of the California Council on the Education of Teachers (CCET), describes how he became involved with the CCET, and how the cultural environment of the CCET shifted from a "Pink Tea" society to one of political action focused on the real needs of the schools for which teachers are prepared. Thompson…

  17. Determination of arsenic concentration in tiger tooth croaker (Otolithes ruber and indian halibut (Psettodes erumei using hydride generation atomic absorption spectrophotometer

    Directory of Open Access Journals (Sweden)

    E Rahimi

    2011-11-01

    Full Text Available Heavy metal contaminants in fish are of particular interest because of their potential risk to human. This study was undertaken to determine the levels of arsenic in two fish type including tiger tooth croaker and Indian halibut  in Esfahan. A total of 42 fish samples including 28 tiger tooth croaker (Otolithes ruber and 14 Indian halibut (Psettodes erumei were collected from retails of Esfahan from May 2010 to January 2011. For detection of arsenic contamination, the edible muscles of  fish samples were analyzed by hydride generation atomic absorption spectrophotometer. The arsenic contamination in fish samples were found to be in the range of 11 to 98 µg/kg. Concentration of arsenic in tiger tooth croaker and Indian halibut was 11-56 and 32-98 µg/kg, respectively. Arsenic concentrations were below the limit was acceptable to the World Health Organization. According to the results, the concentration of arsenic did not exceed the maximum acceptable intake limit.

  18. Removal of polycyclic aromatic hydrocarbons in soil spiked with model mixtures of petroleum hydrocarbons and heterocycles using biosurfactants from Rhodococcus ruber IEGM 231.

    Science.gov (United States)

    Ivshina, Irina; Kostina, Ludmila; Krivoruchko, Anastasiya; Kuyukina, Maria; Peshkur, Tatyana; Anderson, Peter; Cunningham, Colin

    2016-07-15

    Removal of polycyclic aromatic hydrocarbons (PAHs) in soil using biosurfactants (BS) produced by Rhodococcus ruber IEGM 231 was studied in soil columns spiked with model mixtures of major petroleum constituents. A crystalline mixture of single PAHs (0.63g/kg), a crystalline mixture of PAHs (0.63g/kg) and polycyclic aromatic sulfur heterocycles (PASHs), and an artificially synthesized non-aqueous phase liquid (NAPL) containing PAHs (3.00g/kg) dissolved in alkanes C10-C19 were used for spiking. Percentage of PAH removal with BS varied from 16 to 69%. Washing activities of BS were 2.5 times greater than those of synthetic surfactant Tween 60 in NAPL-spiked soil and similar to Tween 60 in crystalline-spiked soil. At the same time, amounts of removed PAHs were equal and consisted of 0.3-0.5g/kg dry soil regardless the chemical pattern of a model mixture of petroleum hydrocarbons and heterocycles used for spiking. UV spectra for soil before and after BS treatment were obtained and their applicability for differentiated analysis of PAH and PASH concentration changes in remediated soil was shown. The ratios A254nm/A288nm revealed that BS increased biotreatability of PAH-contaminated soils. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. The bioenergetic status relates to dopamine neuron loss in familial PD with PINK1 mutations.

    Directory of Open Access Journals (Sweden)

    Rüediger Hilker

    Full Text Available Mutations in the PINK1 gene cause autosomal recessive familial Parkinson's disease (PD. The gene encodes a mitochondrial protein kinase that plays an important role in maintaining mitochondrial function and integrity. However, the pathophysiological link between mutation-related bioenergetic deficits and the degenerative process in dopaminergic neurons remains to be elucidated. We performed phosphorous ((31P and proton ((1H 3-T magnetic resonance spectroscopic imaging (MRSI in 11 members of a German family with hereditary PD due to PINK1 mutations (PARK6 compared to 23 age-matched controls. All family members had prior 18-Fluorodopa (FDOPA positron emission tomography (PET. The striatal FDOPA uptake was correlated with quantified metabolic brain mapping in MRSI. At group level, the heterozygous PINK1 mutation carriers did not show any MRSI abnormalities relative to controls. In contrast, homozygous individuals with manifest PD had putaminal GPC, PCr, HEP and β-ATP levels well above the 2SD range of controls. Across all subjects, the FDOPA K(i values correlated positively with MI (r = 0.879, p<0.001 and inversely with β-ATP (r = -0.784, p = 0.008 and GPC concentrations (r = -0.651, p = 0.030 in the putamen. Our combined imaging data suggest that the dopaminergic deficit in this family with PD due to PINK1 mutations relates to osmolyte dysregulation, while the delivery of high energy phosphates was preserved. Our results corroborate the hypothesis that PINK1 mutations result in reduced neuronal survival, most likely due to impaired cellular stress resistance.

  20. Evidence of Neurobiological Changes in the Presymptomatic PINK1 Knockout Rat.

    Science.gov (United States)

    Ferris, Craig F; Morrison, Thomas R; Iriah, Sade; Malmberg, Samantha; Kulkarni, Praveen; Hartner, Jochen C; Trivedi, Malav

    2018-01-01

    Genetic models of Parkinson's disease (PD) coupled with advanced imaging techniques can elucidate neurobiological disease progression, and can help identify early biomarkers before clinical signs emerge. PTEN-induced putative kinase 1 (PINK1) helps protect neurons from mitochondrial dysfunction, and a mutation in the associated gene is a risk factor for recessive familial PD. The PINK1 knockout (KO) rat is a novel model for familial PD that has not been neuroradiologically characterized for alterations in brain structure/function, alongside behavior, prior to 4 months of age. To identify biomarkers of presymptomatic PD in the PINK1 -/- rat at 3 months using magnetic resonance imaging techniques. At postnatal weeks 12-13; one month earlier than previously reported signs of motor and cognitive dysfunction, this study combined imaging modalities, including assessment of quantitative anisotropy across 171 individual brain areas using an annotated MRI rat brain atlas to identify sites of gray matter alteration between wild-type and PINK1 -/- rats. The olfactory system, hypothalamus, thalamus, nucleus accumbens, and cerebellum showed differences in anisotropy between experimental groups. Molecular analyses revealed reduced levels of glutathione, ATP, and elevated oxidative stress in the substantia nigra, striatum and deep cerebellar nuclei. Mitochondrial genes encoding proteins in Complex IV, along with mRNA levels associated with mitochondrial function and genes involved in glutathione synthesis were reduced. Differences in brain structure did not align with any cognitive or motor impairment. These data reveal early markers, and highlight novel brain regions involved in the pathology of PD in the PINK1 -/- rat before behavioral dysfunction occurs.

  1. PINK1 heterozygous mutations induce subtle alterations in dopamine-dependent synaptic plasticity

    Science.gov (United States)

    Madeo, G.; Schirinzi, T.; Martella, G.; Latagliata, E.C.; Puglisi, F.; Shen, J.; Valente, E.M.; Federici, M.; Mercuri, N.B.; Puglisi-Allegra, S.; Bonsi, P.; Pisani, A.

    2014-01-01

    Background Homozygous or compound heterozygous mutations in the PTEN-induced kinase 1 (PINK1) gene are causative of autosomal recessive, early onset PD. Single heterozygous mutations have been repeatedly detected in a subset of patients as well as in non-affected subjects, and their significance has long been debated. Several neurophysiological studies from non-manifesting PINK1 heterozygotes have shown the existence of neural plasticity abnormalities, indicating the presence of specific endophenotypic traits in the heterozygous state. Methods In the present study, we performed a functional analysis of corticostriatal synaptic plasticity in heterozygous PINK1 knock-out (PINK1+/−) mice by a multidisciplinary approach. Results We found that, despite a normal motor behavior, repetitive activation of cortical inputs to striatal neurons failed to induce long-term potentiation (LTP), whereas long-term depression (LTD) was normal. Although nigral dopaminergic neurons exhibited normal morphological and electrophysiological properties with normal responses to dopamine receptor activation, we measured a significantly lower dopamine release in the striatum of PINK1+/−, compared to control mice, suggesting that a decrease in stimulus-evoked dopamine overflow acts as a major determinant for the LTP deficit. Accordingly, pharmacological agents capable of increasing the availability of dopamine in the synaptic cleft restored a normal LTP in heterozygous mice. Moreover, MAO-B inhibitors rescued a physiological LTP and a normal dopamine release. Conclusions Our results provide novel evidence for striatal plasticity abnormalities even in the heterozygous disease state. These alterations might be considered an endophenotype to this monogenic form of PD, and a valid tool to characterize early disease stage and design possible disease-modifying therapies. PMID:24167038

  2. Silencing of PINK1 expression affects mitochondrial DNA and oxidative phosphorylation in dopaminergic cells.

    Directory of Open Access Journals (Sweden)

    Matthew E Gegg

    Full Text Available Mitochondrial dysfunction has been implicated in the pathogenesis of Parkinson's disease (PD. Impairment of the mitochondrial electron transport chain (ETC and an increased frequency in deletions of mitochondrial DNA (mtDNA, which encodes some of the subunits of the ETC, have been reported in the substantia nigra of PD brains. The identification of mutations in the PINK1 gene, which cause an autosomal recessive form of PD, has supported mitochondrial involvement in PD. The PINK1 protein is a serine/threonine kinase localized in mitochondria and the cytosol. Its precise function is unknown, but it is involved in neuroprotection against a variety of stress signalling pathways.In this report we have investigated the effect of silencing PINK1 expression in human dopaminergic SH-SY5Y cells by siRNA on mtDNA synthesis and ETC function. Loss of PINK1 expression resulted in a decrease in mtDNA levels and mtDNA synthesis. We also report a concomitant loss of mitochondrial membrane potential and decreased mitochondrial ATP synthesis, with the activity of complex IV of the ETC most affected. This mitochondrial dysfunction resulted in increased markers of oxidative stress under basal conditions and increased cell death following treatment with the free radical generator paraquat.This report highlights a novel function of PINK1 in mitochondrial biogenesis and a role in maintaining mitochondrial ETC activity. Dysfunction of both has been implicated in sporadic forms of PD suggesting that these may be key pathways in the development of the disease.

  3. Carbon Monoxide, Nitric Oxide, and Nitrogen Dioxide Levels in Gas Ovens Related to Surface Pinking of Cooked Beef and Turkey.

    Science.gov (United States)

    Cornforth; Rabovitser; Ahuja; Wagner; Hanson; Cummings; Chudnovsky

    1998-01-19

    Carbon monoxide (CO) and total nitrogen oxide (NO(x)()) levels were monitored during meat cookery with a standard Ovenpak and a new ultralow-NO(x)() (ULN) cyclonic gas burner. With the standard burner, CO varied from 103 to 152 ppm, NO(x)() was 1.3-10.7 ppm, and surface pinking was observed on both beef and turkey. The ULN burner at optimal efficiency produced only 6.7 ppm of CO and 1 ppm of NO(x)(), insufficient to cause surface pinking. To determine the relative contribution of CO and NO(x)() to pinking, trials were also conducted in an electric oven with various pure gases. Pinking was not observed with up to 149 ppm of CO or 5 ppm of NO. However, as little as 0.4 ppm of nitrogen dioxide (NO(2)) caused pinking of turkey rolls. Beef roasts were pink at >2.5 ppm of NO(2). Thus, pinking previously attributed to CO and NO in gas ovens is instead due to NO(2), which has much greater reactivity than NO with moisture at meat surfaces.

  4. Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration.

    Directory of Open Access Journals (Sweden)

    Suzana Gispert

    Full Text Available BACKGROUND: Parkinson's disease (PD is an adult-onset movement disorder of largely unknown etiology. We have previously shown that loss-of-function mutations of the mitochondrial protein kinase PINK1 (PTEN induced putative kinase 1 cause the recessive PARK6 variant of PD. METHODOLOGY/PRINCIPAL FINDINGS: Now we generated a PINK1 deficient mouse and observed several novel phenotypes: A progressive reduction of weight and of locomotor activity selectively for spontaneous movements occurred at old age. As in PD, abnormal dopamine levels in the aged nigrostriatal projection accompanied the reduced movements. Possibly in line with the PARK6 syndrome but in contrast to sporadic PD, a reduced lifespan, dysfunction of brainstem and sympathetic nerves, visible aggregates of alpha-synuclein within Lewy bodies or nigrostriatal neurodegeneration were not present in aged PINK1-deficient mice. However, we demonstrate PINK1 mutant mice to exhibit a progressive reduction in mitochondrial preprotein import correlating with defects of core mitochondrial functions like ATP-generation and respiration. In contrast to the strong effect of PINK1 on mitochondrial dynamics in Drosophila melanogaster and in spite of reduced expression of fission factor Mtp18, we show reduced fission and increased aggregation of mitochondria only under stress in PINK1-deficient mouse neurons. CONCLUSION: Thus, aging Pink1(-/- mice show increasing mitochondrial dysfunction resulting in impaired neural activity similar to PD, in absence of overt neuronal death.

  5. The effect of gamma irradiation on the growth and flowering of stem chrysanthemum (chrysanthemum morifolium ramat.) cv. pink fiji

    International Nuclear Information System (INIS)

    Ita Dwimahyani

    2007-01-01

    Rooted shoot cuttings have been irradiated by gamma rays with 0, 10, 15, 20 and 25 Gy doses, and then planted in green house on Gadog, Ciawi. Plant growth from each doses proved to be varied, both in height and flowering process. Plants with dose 15 Gy and higher had shown late flowering time and dwarfing. The optimal dose for chrysanthemum cv. Pink Fiji irradiation is suggested to be 10-15 Gy. The highest mutation frequency shown on flower color is yellow, followed by orange, white, dark pink and light pink. (author)

  6. Effect of weather factors on the incidence and development of pink bollworm on flowers of advance cotton genotypes

    International Nuclear Information System (INIS)

    Khaliq, A.; Subhani, M.N.; Hassan, S.W.; Afzal, M.

    2008-01-01

    Ten advance genotypes of cotton Viz. BH-121, NIAB KRISHMA, DNH-137, VH-142, VH-142 BH-125, MNH-635, SLH-267, FNH-245, CRIS-467 and CRIS-82 were used to determine the effect of different weather factors on the incidence and development of pink bollworm (Pectinophora gossyiella) infestation at Nuclear institute for Agriculture and Biology (NIAB) Faisalabad. Trials were laid out using Randomized Complete Block Design (RCBD) with four replications. Finally data were subjected to statistical analysis and for correlation studies between weather factors and pink bollworm. Temperature and relative humidity and rainfall affected negatively for the infestation of pink bollworm on flowers in advance genotypes of cotton. (author)

  7. The human PINK1 locus is regulated in vivo by a non-coding natural antisense RNA during modulation of mitochondrial function

    Directory of Open Access Journals (Sweden)

    Wahlestedt Claes

    2007-03-01

    Full Text Available Abstract Background Mutations in the PTEN induced putative kinase 1 (PINK1 are implicated in early-onset Parkinson's disease. PINK1 is expressed abundantly in mitochondria rich tissues, such as skeletal muscle, where it plays a critical role determining mitochondrial structural integrity in Drosophila. Results Herein we characterize a novel splice variant of PINK1 (svPINK1 that is homologous to the C-terminus regulatory domain of the protein kinase. Naturally occurring non-coding antisense provides sophisticated mechanisms for diversifying genomes and we describe a human specific non-coding antisense expressed at the PINK1 locus (naPINK1. We further demonstrate that PINK1 varies in vivo when human skeletal muscle mitochondrial content is enhanced, supporting the idea that PINK1 has a physiological role in mitochondrion. The observation of concordant regulation of svPINK1 and naPINK1 during in vivo mitochondrial biogenesis was confirmed using RNAi, where selective targeting of naPINK1 results in loss of the PINK1 splice variant in neuronal cell lines. Conclusion Our data presents the first direct observation that a mammalian non-coding antisense molecule can positively influence the abundance of a cis-transcribed mRNA under physiological abundance conditions. While our analysis implies a possible human specific and dsRNA-mediated mechanism for stabilizing the expression of svPINK1, it also points to a broader genomic strategy for regulating a human disease locus and increases the complexity through which alterations in the regulation of the PINK1 locus could occur.

  8. Modelling planktic foraminifer growth and distribution using an ecophysiological multi-species approach

    DEFF Research Database (Denmark)

    Lombard, Fabien; Labeyrie, L.; Michel, E.

    2011-01-01

    We present an eco-physiological model reproducing the growth of eight foraminifer species (Neogloboquadrina pachyderma, Neogloboquadrina incompta, Neogloboquadrina dutertrei, Globigerina bulloides, Globigerinoides ruber, Globigerinoides sacculifer, Globigerinella siphonifera and Orbulina universa...... in the marine carbon cycle....... ocean (PISCES) instead of satellite images as forcing variables gives also good results, but with lower efficiency (58.9%). Compared to core tops observations, the model also correctly reproduces the relative worldwide abundance and the diversity of the eight species when using either satellite data...

  9. AFSC/ABL: Adult Pink Salmon Predation in Prince William Sound and Southeast Alaska, 2009-2011

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The project objectives were to assess potential salmon predation impact on juvenile salmon and herring by: (1) comparing diets of adult pink salmon during their...

  10. Growth kinetics and efficacy as parameters for ranking and selecting biocontrol agents that reduce pink rot in stored potatoes

    Science.gov (United States)

    Increased production of organic agricultural products and the relative ineffectiveness of traditional control measures support development of new biocontrol technologies for use against pink rot infections in storage. The microbiota of 84 different agricultural soils was individually transferred to...

  11. Improvement of age estimation using amino acid racemization in a case of pink teeth.

    Science.gov (United States)

    Ohtani, S; Yamada, Y; Yamamoto, I

    1998-03-01

    Age was estimated from pink teeth using racemization of dentinal aspartic acid. Materials for identification were two lower second premolars. The body was determined to be that of a 40-year-old man; however, the age of the decedent had been estimated to be 29 and 30 years by the conventional method and 30 years from findings in the oral cavity. To clarify the cause of this difference, the powdered teeth were further washed in 0.01 mol/L hydrochloric acid. The racemization ratio (D/L ratio) of ordinary white teeth from persons of known age was slightly lower than that before washing, whereas that of the teeth used for identification was higher than before washing. The calculated age of the decedent using the racemization ratio of his teeth was between 36 and 37 years. These results suggest that age estimated from pink teeth is probably underestimated, but a more accurate age estimate can be obtained after adequate washing.

  12. Heterozygous carriers of a Parkin or PINK1 mutation share a common functional endophenotype

    DEFF Research Database (Denmark)

    van Nuenen, BF; Siebner, Hartwig; Weiss, MM

    2008-01-01

    inherited Parkinson disease alters the cortical control of sequential finger movements. METHODS: Nonmanifesting individuals carrying a single heterozygous Parkin (n = 13) or PINK1 (n = 9) mutation and 23 healthy controls without these mutations were studied with functional MRI (fMRI). During f...... rostral dorsal premotor cortex in mutation carriers but not in controls. Task-related activation of these premotor areas was similar in carriers of a Parkin or PINK1 mutation. CONCLUSION: Mutations in different genes linked to recessively inherited Parkinson disease are associated with an additional...... recruitment of rostral supplementary motor area and rostral dorsal premotor cortex during a simple motor sequence task. These premotor areas were recruited independently of the underlying genotype. The observed activation most likely reflects a "generic" compensatory mechanism to maintain motor function...

  13. Oxidative stability of pork emulsion containing tomato products and pink guava pulp during refrigerated aerobic storage.

    Science.gov (United States)

    Joseph, Serlene; Chatli, Manish K; Biswas, Ashim K; Sahoo, Jhari

    2014-11-01

    Lipid oxidation-induced quality problems can be minimized with the use of natural antioxidants. Antioxidant potential of tomato puree (10 %; T-1), tomato pulp (12.5 %; T-2), lyophilized tomato peel (6 %; T-3), and pink guava pulp (10 %; T-4) was evaluated in raw pork emulsion during refrigerated storage for 9 days under aerobic packaging. The lycopene and β-carotene content varied in pork emulsion as T-3 > T-1 > T-2 > T-4 and decreased (P emulsions than in control. Overall, incorporation of tomato products and pink guava pulp improved the visual colour and odour scores of raw pork emulsion. These results indicated that tomato products and guava pulp can be utilized as sources of natural antioxidants in raw pork products to minimize lipid oxidation, off-odour development, and surface discolouration.

  14. The Pinking Shears: A Novel Tool for Improving Skin Graft Cosmesis

    Directory of Open Access Journals (Sweden)

    Cormac W. Joyce, MB BCh

    2014-12-01

    Full Text Available Summary: A significant aesthetic disadvantage to split skin grafts is the obvious transition between the graft and the normal skin. We report on a novel method to interrupt this transition point by using pinking shears, which are dressmaking scissors with saw-toothed blades that create a chevron pattern instead of a straight edge. We describe a case where the pinking shears were utilized on a split skin graft and Integra for reconstruction of the skin on a volar forearm. This technique allows for breaking-up of the transition point between the skin graft and normal skin and gives rise to an improved aesthetic outcome as the boundary is significantly less well-defined. This novel method shows promise and further study is certainly warranted.

  15. Asymmetric hybridization and introgression between pink salmon and chinook salmon in the Laurentian Great Lakes

    Science.gov (United States)

    Rosenfield, Jonathan A.; Todd, Thomas; Greil, Roger

    2000-01-01

    Among Pacific salmon collected in the St. Marys River, five natural hybrids of pink salmon Oncorhynchus gorbuscha and chinook salmon Oncorhynchus tshawytscha and one suspected backcross have been detected using morphologic, meristic, and color evidence. One allozyme (LDH, l-lactate dehydrogenase from muscle) and one nuclear DNA locus (growth hormone) for which species-specific fixed differences exist were analyzed to detect additional hybrids and to determine if introgression had occurred. Restriction fragment length polymorphism of mitochondrial DNA (mtDNA) was used to identify the maternal parent of each hybrid. Evidence of introgression was found among the five previously identified hybrids. All hybrid specimens had chinook salmon mtDNA, indicating that hybridization between chinook salmon and pink salmon in the St. Marys River is asymmetric and perhaps unidirectional. Ecological, physiological, and sexual selection forces may contribute to this asymmetric hybridization. Introgression between these highly differentiated species has implications for management, systematics, and conservation of Pacific salmon.

  16. Analysis of the Exxon Valdez oil spill impacts on Prince William Sound pink salmon

    International Nuclear Information System (INIS)

    Brannon, E.L.; Moulton, L.L.; Maki, A.W.

    1996-01-01

    The impact of the Exxon Valdez oil spill on pink salmon in Prince William Sound (PWS), was studied. Since the incident, numerous and extensive field and laboratory studies have been completed by various scientists. This paper synthesized information on the post-spill salmon harvests, the concentration of petroleum that entered the water column, the number of streams oiled, and stream sediment chemistry, to determine the potential for oil spill effects. Results from this study showed that oiling to the extent experienced during incubation and rearing during both the spring and fall of 1989 through the spring of 1991, resulted in no measurable effects on the PWS pink salmon. The estimated losses predicted earlier, likely exceeded actual losses. 16 refs., 4 tabs., 4 figs

  17. Adaptive harvest management for the Svalbard population of Pink-Footed Geese: 2014 progress summary

    Science.gov (United States)

    Johnson, Fred A.; Madsen, J.

    2015-01-01

    This document describes progress to date on the development of an adaptive harvest-management strategy for maintaining the Svalbard population of pink-footed geese (Anser brachyrhynchus) near their agreed target level (60 thousand) by providing for sustainable harvests in Norway and Denmark.  Specifically, this report provides an assessment of the most recent monitoring information and its implications for the harvest management strategy.

  18. Efficacy of pink guava pulp as an antioxidant in raw pork emulsion

    OpenAIRE

    Joseph, Serlene; Chatli, Manish K.; Biswas, Ashim K.; Sahoo, Jhari

    2012-01-01

    Lipid oxidation-induced quality problems can be minimized with the use of natural antioxidants. The antioxidant potential of pink guava pulp (PGP) was evaluated at different levels (0%; C, 5.0%; T-1, 7.5%; T-2 and 10.0%; T-3) in the raw pork emulsion during refrigerated storage of 9 days under aerobic packaging. Lycopene and β-carotene contents increased (P 

  19. Complete Genome Sequence of Methylobacterium populi P-1M, Isolated from Pink-Pigmented Household Biofilm.

    Science.gov (United States)

    Morohoshi, Tomohiro; Ikeda, Tsukasa

    2016-06-16

    Methylobacterium populi P-1M is isolated from the pink-pigmented household biofilm. Here, we present the complete genome sequence of P-1M, consisting of one chromosome of 5,705,640 bp and five plasmids of 64,864 bp, 59,879 bp, 42,569 bp, 41,417 bp, and 29,506 bp. Copyright © 2016 Morohoshi and Ikeda.

  20. Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria.

    Science.gov (United States)

    Doronina, Nina V; Trotsenko, Yuri A; Kuznetsov, Boris B; Tourova, Tatjana P; Salkinoja-Salonen, Mirja S

    2002-05-01

    Two aerobic, pink-pigmented, facultatively methylotrophic bacteria, strains F20T and RXM(T), are described taxonomically. On the basis of their phenotypic and genotypic properties, the isolates are proposed as novel species of the genus Methylobacterium, Methylobacterium suomiense sp. nov. (type strain F20T = VKM B-2238T = NCIMB 13778T) and Methylobacterium lusitanum sp. nov. (type strain RXMT = VKM B-2239T = NCIMB 13779T).

  1. Albumin Overload and PINK1/Parkin Signaling-Related Mitophagy in Renal Tubular Epithelial Cells.

    Science.gov (United States)

    Tan, Jin; Xie, Qi; Song, Shuling; Miao, Yuyang; Zhang, Qiang

    2018-03-01

    BACKGROUND Albumin, as a major urinary protein component, is a risk factor for chronic kidney disease progression. Mitochondrial dysfunction is one of the main causes of albumin-induced proximal tubule cells injury. Mitophagy is considered as a pivotal protective mechanism for the elimination of dysfunctional mitochondria. The objective of this research was to determine whether albumin overload-induced mitochondrial dysfunction can activate PINK1/Parkin-mediated mitophagy in renal tubular epithelial cells (TECs). MATERIAL AND METHODS Immunofluorescence assay and Western blot assay were used to detect the effects of albumin overload on autophagy marker protein LC3. Transmission electron microscopy and Western blot assay were used to investigate the role of albumin in mitochondrial injury. Western blot assay and co-localization of acidic lysosomes and mitochondria assay were employed to detect the activation of mitophagy induced by albumin. Finally, we explored the role of PINK1/Parkin signaling in albumin-induced mitophagy by inhibiting mitophagy by knockdown of PARK2 (Parkin) level. RESULTS Immunofluorescence and Western blot results showed that the expression level of LC3-II increased, and the maximum increase point was observed after 8 h of albumin treatment. Transmission electron microscopy results demonstrated that albumin overload-induced mitochondrial injury and quantity of autophagosomes increased. Additionally, expression of PINK1 and cytosolic cytochrome C increased and mitochondria cytochrome C decreased in the albumin group. The co-localization of acidic lysosomes and mitochondria demonstrated that the number of albumin overload-induced mitophagy-positive dots increased. The transient transfection of PARK2 siRNA result showed knockdown of the expression level of PARK2 can inhibit mitophagy induced by albumin. CONCLUSIONS In conclusion, our study suggests that mitochondrial dysfunction activates the PINK1/Parkin signaling and mitophagy in renal tubular

  2. Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.

    OpenAIRE

    Dourado, Manuella Nóbrega; Aparecida Camargo Neves, Aline; Santos, Daiene Souza; Araújo, Welington Luiz

    2015-01-01

    The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM) bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one carbon (C1), such as methanol and methylamine. Due to their high phenotypic plasticity, these bacteria are able to colonize different habitats, such as soil, water, and sediment, and different host plants as both endophytes and epiphytes. In plant colonization, the frequency and distribution may be in...

  3. Growth and enzymological characteristics of a pink-pigmented facultative methylotroph Methylobacterium sp. MB1.

    Science.gov (United States)

    Baev, M V; Kuznetsov, E V; Skladnev, D A; Govorukhina, N I; Sterkin, V E; Tsygankov, Y D

    1992-01-01

    Growth characteristics of batch and continuous cultures of the pink facultative methylotroph Methylobacterium sp. MB1 were determined. The response of a chemostat culture to a pulse increase of methanol concentration was studied. Malate, succinate and oxaloacetate additions to the methanol-supplemented medium decreased batch culture growth inhibition by methanol. The carotenoid content in cells grown in a chemostat decreased with increasing growth rate. The key enzyme activities of C1-metabolism were measured in a chemostat culture at different dilution rates.

  4. DNA:DNA hybridization studies on the pink-pigmented facultative methylotrophs.

    Science.gov (United States)

    Hood, D W; Dow, C S; Green, P N

    1987-03-01

    The genomic relatedness among 36 strains of pink-pigmented facultatively methylotrophic bacteria (PPFMs) was estimated by determination of DNA base composition and by DNA:DNA hybridization studies. A reproducible hybridization system was developed for the rapid analysis of multiple DNA samples. Results indicated that the PPFMs comprise four major and several minor homology groups, and that they should remain grouped in a single genus, Methylobacterium.

  5. Common variants in the PARL and PINK1 genes increase the risk to leprosy in Han Chinese from South China.

    Science.gov (United States)

    Wang, Dong; Zhang, Deng-Feng; Feng, Jia-Qi; Li, Guo-Dong; Li, Xiao-An; Yu, Xiu-Feng; Long, Heng; Li, Yu-Ye; Yao, Yong-Gang

    2016-11-23

    Leprosy is a chronic infectious and neurological disease caused by Mycobacterium leprae, an unculturable pathogen with massive genomic decay and dependence on host metabolism. We hypothesized that mitochondrial genes PARL and PINK1 would confer risk to leprosy. Thirteen tag SNPs of PARL and PINK1 were analyzed in 3620 individuals with or without leprosy from China. We also sequenced the entire exons of PARL, PINK1 and PARK2 in 80 patients with a family history of leprosy by using the next generation sequencing technology (NGS). We found that PARL SNP rs12631031 conferred a risk to leprosy (P adjusted  = 0.019) and multibacillary leprosy (MB, P adjusted  = 0.020) at the allelic level. rs12631031 and rs7653061 in PARL were associated with leprosy and MB (dominant model, P adjusted  leprosy at the genotypic level (P adjusted  = 0.004). We confirmed that common variants in PARL and PINK1 were associated with leprosy in patients underwent NGS. Furthermore, PARL and PINK1 could physically interact with each other and were involved in the highly connected network formed by reported leprosy susceptibility genes. Together, our results showed that PARL and PINK1 genetic variants are associated with leprosy.

  6. A qualitative exploration of patients' attitudes towards the 'Participate Inform Notice Know' (PINK) patient safety video.

    Science.gov (United States)

    Pinto, Anna; Vincent, Charles; Darzi, Ara; Davis, Rachel

    2013-02-01

    To explore patients' attitudes towards the PINK video, a patient education video aimed at encouraging hospital patients' involvement in safety-relevant behaviours. Qualitative semi-structured interviews. Detailed field notes were taken during the interviews which were analysed using content analysis. One National Health System (NHS) teaching hospital based in London, UK. Thirty-six in-patients aged between 20 and 79 years, 18 of them males. The PINK video is a short animated educational video aimed at encouraging patients to be involved in the safety of their care during hospitalization. Patients' perceptions of how informative, relevant and acceptable the video is; attitudes towards participating in the recommended safety-related behaviours and; potential negative side effects of watching the video. Overall the video was received favourably among the interviewees. Commonly cited benefits included raising awareness and facilitating patients to be involved in their care during their hospital stay. More variability was found in participants' views with regard to the video's role as a patient safety enhancement tool. A number of suggestions for improvement of the video were provided relating to tailoring its content and design to meet the needs of individual patients and their circumstances. Educational videos such as PINK have significant potential to empower patients in the safety and quality of their care. However, efforts to implement patient safety educational videos in practice need to consider different patient groups' needs and characteristics instead of trying to adopt 'a one size fits all' approach.

  7. Recovery of polyphenols from Pink Guava processing wastes by ultra filtration

    International Nuclear Information System (INIS)

    Lilis Sukeksi; Che Rosmani Che Hassan; Nik Meriam Sulaiman; Mohamed Kheireddine Aroua

    2010-01-01

    Full text: Processing of fruits are results in high amounts of waste material that is prone to microbial spoilage and usually represents a problem that is further aggravated by legal restrictions. Polyphenols are a wide variety of compounds that occur in pink guava fruit or others fruits and vegetables. Recovery from pink guava wastes seems to be promising in the case of polyphenols, which are of considerable interest due to their healthy and anti oxidative properties. In this work the performance of commercial tubular PVDF membrane FP 200 with nominal MWCO 200,000, was studied during pretreatment for recovery polyphenols from pink guava processing wastes. The experiments have been carried out at trans-membrane pressure of 0.5 until 2.5 Bar, and all permeate flux significantly decreased with time until a steady-state was established. The steady-state permeates flux reached a maximum at a trans-membrane pressure of about 1 bar. The first results obtained confirm the flux decline at 20 minutes was 35 % of the total flux. Meanwhile concentration of polyphenols at first step reached a steady state after 900 ml of permeate volume (47 %) and the concentration of polyphenols when the permeate volume at VCR = 4 or 3000 ml is 54 %. (author)

  8. Growth, Plastochron, and the Final Number of Nodes of China Pink Seedlings Grown on Different Substrates

    Directory of Open Access Journals (Sweden)

    Marília Milani

    Full Text Available ABSTRACT The objective of this work was to plot the growth curves and determine the plastochron and the final number of nodes of China pink seedlings grown on different substrates. Thus, 392 China pink seedlings were grown on seven substrates under greenhouse conditions, in Santa Maria in the state of Rio Grande do Sul, Brazil. The growth curves were plotted using the logistic model. The plastochron was estimated by the inverse of the angular coefficient of the simple linear regression between the number of accumulated nodes and accumulated thermal sum from the subsampling of the seedlings. In all substrates, the logistic model fit better for the variable number of leaves than for the plant height. The plants in substrates with 50% of soil plus 50% of rice husk ash, and 80% of rice husk ash plus 20% earthworm humus had the longest cycles with 74 and 65 days, respectively. They completed the cycles with a thermal sum of 1317.9 ºC day for number of leaves and plant height. The growth curves that were plotted by the logistic model and the plastochron of the China pink seedlings are dependent on the type of substrate used. The commercial substrate Mecplant® had the best results. The average final number of nodes of the main stem of the plants was 14 for all substrates.

  9. An experiment on the reuse of artificial medium for rearing Pink Bollworm, Pectinophora gossypiella (Saunders)

    International Nuclear Information System (INIS)

    Chiravathanapong, S.

    1983-12-01

    The development of pink bollworm, Pectinophora gossypiella (Saunders) was compared on rearing on used-diet or the medium remaining after larval use, with no supplements added versus used-diet with 100 percent supplement of vitamins, choline chloride and the heat sensitive antimicrobial agents (Fumidil B, Aureomycin and formaldehyde). In another evaluation of used diet it was mixed at 0, 25, 50, and 75 percent with the new diet and compared to 100 percent new diet. Used diet with no supplement and used diet with 100 percent supplement of vitamins and heat sensitive antimicrobial agents had no significant effect on the development of the pink bollworm except in adult emergence. Some pupae which developed from unsupplemented used-diet died before moth emergence. Used diet mixed with 75 percent new ingredients was the best rate for reclamation of the used diet and produced the same results in pupal production rate pupal weight, adult emergence, adult longevity and egg production of female pink bollworm when compared to those from diet prepared with 100 percent new ingredients. The other rates supplemented with 0,25 and 50 percent new ingredients were significantly poorer by most criteria

  10. Laboratory evidence for short and long-term damage to pink salmon incubating in oiled gravel

    International Nuclear Information System (INIS)

    Heintz, R.; Rice, S.; Wiedmer, M.

    1995-01-01

    Pink salmon, incubating in gravel contaminated with crude oil, demonstrated immediate and delayed responses in the laboratory at doses consistent with the concentrations observed in oiled streams in Prince William Sound. The authors incubated pink salmon embryos in a simulated intertidal environment with gravel contaminated by oil from the Exxon Valdez. During the incubation and emergence periods the authors quantified dose-response curves for characters affected directly by the oil. After emergence, fish were coded wire tagged and released, or cultured in netpens. Delayed responses have been observed among the cultured fish, and further observations will be made when coded wire tagged fish return in September 1995. The experiments have demonstrated that eggs need not contact oiled gravel to experience increased mortality, and doses as low as 17 ppb tPAH in water can have delayed effects on growth. A comparison of sediment tPAH concentrations from streams in Prince William Sound with these laboratory data suggests that many 1989 brood pink salmon were exposed to deleterious quantities of oil

  11. The effect of irradiation in the preservation of pink pepper (Schinus terebinthifolius Raddi)

    International Nuclear Information System (INIS)

    Régia Marques de Souza, Adriana; Arthur, Valter; Pires Nogueira, Danielle

    2012-01-01

    Pink peppers, also known as “pimenta-rosa” and “poivre rose”, are the fruit of Schinus terebinthifolius Raddi, a species of pepper cultivated in Brazil, and have great potential for the exploration of uses. In efforts to lengthen the shelf life of this pepper, the purpose of this study was to evaluate the effect of different doses of radiation on its physical composition and color. The pink pepper samples were irradiated with doses of 0, 0.2, 0.4, 0.8 and 1.6 kGy, and the moisture, ash and lipid contents, pH and color were analyzed. The moisture content, lipid content and pH analysis indicated effects due to the irradiation (p>0.05) in which the higher doses resulted in decreases in the attribute. In contrast, there were no significant differences for the ash analysis (p ⁎ and b ⁎ were the most affected by the intermediate doses (0.2 and 0.8 kGy), which induced their elevation, enhancing the reddish and yellowish colors. Based on the presented data, irradiation is as an alternative preservation process for pink peppers.

  12. Loss of PINK1 attenuates HIF-1α induction by preventing 4E-BP1-dependent switch in protein translation under hypoxia.

    Science.gov (United States)

    Lin, William; Wadlington, Natasha L; Chen, Linan; Zhuang, Xiaoxi; Brorson, James R; Kang, Un Jung

    2014-02-19

    Parkinson's disease (PD) has multiple proposed etiologies with implication of abnormalities in cellular homeostasis ranging from proteostasis to mitochondrial dynamics to energy metabolism. PINK1 mutations are associated with familial PD and here we discover a novel PINK1 mechanism in cellular stress response. Using hypoxia as a physiological trigger of oxidative stress and disruption in energy metabolism, we demonstrate that PINK1(-/-) mouse cells exhibited significantly reduced induction of HIF-1α protein, HIF-1α transcriptional activity, and hypoxia-responsive gene upregulation. Loss of PINK1 impairs both hypoxia-induced 4E-BP1 dephosphorylation and increase in the ratio of internal ribosomal entry site (IRES)-dependent to cap-dependent translation. These data suggest that PINK1 mediates adaptive responses by activating IRES-dependent translation, and the impairments in translation and the HIF-1α pathway may contribute to PINK1-associated PD pathogenesis that manifests under cellular stress.

  13. Linoleic acid enhance the production of moncolin K and red pigments in Monascus ruber by activating mokH and mokA, and by accelerating cAMP-PkA pathway.

    Science.gov (United States)

    Huang, Jing; Liao, NanQing; Li, HaoMing

    2018-04-01

    Monacolin K, an inhibitor of HMG-CoA reductase, is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus ruber. The mokH gene encoding Zn(II)2Cys6 binding protein and mokA gene encoding polyketide synthase are presumed to activate monacolin K production. In this study, linoleic acid could be a quorum sensing signaling molecule to increase monacolin K production in the cyclic AMP(cAMP)-protein kinase A(PKA) signaling pathway. Analysis of the PKA activity and the cAMP concentration shows that linoleic acid could increase cAMP concentration and activate PKA. Analysis of the RT-qPCR products demonstrates that 256μM and 512μM linoleic acid can up-regulate mokH and mokA gene transcript levels. Especially with 512μM linoleic acid addition, linoleic acid increase 1.35 folds of monacolin K production, but 64μM linoleic acid increase 1.94 folds of red pigment production in Monascus ruber. These results show the cAMP-PkA pathway activity can up-regulate mokA and mokH gene, which enhance the yield of Monacolin K. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Identification of candidate amino acids involved in the formation of pink-red pigments in onion (Allium cepa L.) juice and separation by HPLC.

    Science.gov (United States)

    Lee, Eun Jin; Yoo, Kil Sun; Patil, Bhimanagouda S

    2010-10-01

    The formation of pink-red pigments ("pinking") by various amino acids was investigated by reacting amino acids with compounds present in onion juice. The unknown pink-red pigments were generated and separated using high-performance liquid chromatography (HPLC) and a diode array detector (DAD) in the range of 200 to 700 nm. To generate pink-red pigments, we developed several reaction systems using garlic alliinase, purified 1-propenyl-L-cysteine sulfoxide (1-PeCSO), onion thiosulfinate, natural onion juice, and 21 free amino acids. The compound 1-PeCSO was a key compound associated with pinking in the presence of both the alliinase and amino acids. Numerous naturally occurring pink-red pigments were detected and separated from pink onion juice using the HPLC-DAD system at 515 nm. Most free amino acids, with the exceptions of histidine, serine, and cysteine, formed various pink-red pigments when reacted with onion thiosulfinate. This observation indicated that onion pinking is caused not by a single pigment, but by many. Furthermore, more than one color compound could be produced from a single amino acid; this explains, in part, why there were many pink-red compound peaks in the chromatogram of discolored natural onion juice. We presumed that the complexity of the pink-red pigments was due to the involvement of more than 21 natural amino acids as well as several derivatives of the color products produced from each amino acid. We observed that the pinking process in onion juice is very similar to that of the greening process in crushed garlic, emphasizing that both thiosulfinate from flavor precursors and free amino acids are absolutely required for the discoloration.

  15. LncRNA NEAT1 promotes autophagy in MPTP-induced Parkinson's disease through stabilizing PINK1 protein.

    Science.gov (United States)

    Yan, Wang; Chen, Zhao-Ying; Chen, Jia-Qi; Chen, Hui-Min

    2018-02-19

    Long non-coding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) was found to be closely related to the pathological changes in brain and nervous system. However, the role of NEAT1 and its potential mechanism in Parkinson's disease (PD) largely remain uncharacterized. In this study, PD mouse model was established by intraperitoneal injection of MPTP. The numbers of TH + neurons, NEAT1 expression and the level of PINK1, LC3-II, LC3-I protein were assessed in PD mice. SH-SY5Y cells were treated with MPP + as PD cell model. RNA pull-down assay was used to identify the interaction between NEAT1 and PINK1 in vitro. The endogenous expression of NEAT1 was modified by lentiviral vector carrying interference sequence for NEAT1 in vivo. The numbers of TH + neurons significantly decreased in PD mice compared with the control. The expressions of NEAT1, PINK1 protein and LC3-II/LC3-I level were increased by MPTP in vitro and in vivo. Moreover, NEAT1 positively regulated the protein level of PINK1 through inhibition of PINK1 protein degradation. And NEAT1 mediated the effects of MPP + on SH-SY5Y cells through stabilization of PINK1 protein. The results of in vivo experiments revealed that NEAT1 knockdown could effectively suppress MPTP-induced autophagy in vivo that alleviated dopaminergic neuronal injury. LncRNA NEAT1 promoted the MPTP-induced autophagy in PD through stabilization of PINK1 protein. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Identification of pink-pigmented bacteria isolated from environmental water samples and their biofilm formation abilities.

    Science.gov (United States)

    Furuhata, Katsunori; Kato, Yuko; Goto, Keiichi; Saitou, Keiko; Sugiyama, Jun-Ichi; Hara, Motonobu; Fukuyama, Masahumi

    2008-06-01

    Sixty-seven strains of pink-pigmented bacteria, which were isolated from environmental water samples collected nationwide, were identified by partial 16S rDNA sequence analysis. In addition, the biofilm formation ability of the isolates was experimentally investigated. We could identify only 2 strains at the species level: Pedobacter roseus HS-38 and Runella slithyformis HS-77. The results showed that of the strains tested, 22 strains (32.8%) were Pedobacter spp., which was most frequently identified, followed by 19 strains (28.4%) of Arcicella spp., 16 strains (23.9%) of Deinococcus spp., 5 strains (7.5%) of Roseomonas spp., 4 strains (6.0%) of Flectobacillus spp. and 1 strain (1.5%) of Runella sp. Most isolates showed low similarity values to previously known species, and they were found to be novel species. At a result, it was difficult to identify environmental water-derived pink-pigmented bacteria at the species level. On the other hand, when we measured the absorbance by the crystal violet staining to examine the quantities of biofilm formation of these strains, fifty-five (82.0%) of the 67 isolates formed biofilm. The absorbance of Deinococcus sp. HS-75 was the highest (3.56). When comparing the absorbance values among the genera, Roseomonas spp. showed the highest absorbance (mean:1.62), followed by Deinococcus spp. (mean: 1.03), and Arcicella spp. (mean: 1.01). Strains of Flectobacillus spp. (mean: 0.48) and Pedobacter spp. (mean: 0.42) showed lower absorbance values. As above, it was shown that, at the species level, the pink-pigmented bacteria in the water in the Japanese environment had various levels of ability to form biofilm.

  17. Increased frequency of pink bollworm resistance to Bt toxin Cry1Ac in China.

    Directory of Open Access Journals (Sweden)

    Peng Wan

    Full Text Available Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt kill some key insect pests, but evolution of resistance by pests can reduce their efficacy. The main approach for delaying pest adaptation to Bt crops uses non-Bt host plants as "refuges" to increase survival of susceptible pests. To delay evolution of pest resistance to transgenic cotton producing Bt toxin Cry1Ac, the United States and some other countries have required refuges of non-Bt cotton, while farmers in China have relied on "natural" refuges of non-Bt host plants other than cotton. The "natural" refuge strategy focuses on cotton bollworm (Helicoverpa armigera, the primary target of Bt cotton in China that attacks many crops, but it does not apply to another major pest, pink bollworm (Pectinophora gossypiella, which feeds almost entirely on cotton in China. Here we report data showing field-evolved resistance to Cry1Ac by pink bollworm in the Yangtze River Valley of China. Laboratory bioassay data from 51 field-derived strains show that the susceptibility to Cry1Ac was significantly lower during 2008 to 2010 than 2005 to 2007. The percentage of field populations yielding one or more survivors at a diagnostic concentration of Cry1Ac increased from 0% in 2005-2007 to 56% in 2008-2010. However, the median survival at the diagnostic concentration was only 1.6% from 2008 to 2010 and failure of Bt cotton to control pink bollworm has not been reported in China. The early detection of resistance reported here may promote proactive countermeasures, such as a switch to transgenic cotton producing toxins distinct from Cry1A toxins, increased planting of non-Bt cotton, and integration of other management tactics together with Bt cotton.

  18. Electron paramagnetic resonance of atomic hydrogen (H0) centers in pink tourmaline from Brazil

    International Nuclear Information System (INIS)

    Camargo, M.B.

    1985-01-01

    A model for explaining the atom of hydrogen (H 0 ) in pink tourmaline irradiated with gamma rays is presented. The concentration of H 0 was evaluated and the H 0 lines using the electron paramagnetic resonance were analysed. The g factor and the hyperfine interaction constant were measured with accuracy and determined by matrix diagonalization of spin hamiltonian in vetor space of four dimensions, followed by an iterative calculation with quick convergence the local electric field produced by charges in the lattice was calculated and compared with the value obtained experimentally. (M.C.K.) [pt

  19. Isolation and Partial Characterization of the Pink and Blue Pigments of Pocilloporid and Acroporid Corals.

    Science.gov (United States)

    Dove, S G; Takabayashi, M; Hoegh-Guldberg, O

    1995-12-01

    The compounds responsible for the pink and blue colors of two families of hermatypic corals (Pocilloporidae, Acroporidae) from the southern Great Barrier Reef were isolated and biochemically characterized. Isolation of the pink pigment from Pocillopora damicornis (named pocilloporin, {lambda}max = 560 nm, 390 nm) revealed that it was a hydrophilic protein dimer with a native molecular weight of approximately 54 kD and subunits of 28 kD. The subunits are not linked by disulfide bonds. Attempts to dissociate the chromophore from the protein proved unsuccessful. Denaturing the protein with heat (60{deg}C) or 5% sodium dodecyl sulfate (SDS) removed the 560-nm absorbance peak without introducing a detectable bathochromic shift. In acetone, ethanol, ether, and chloroform, the pigment precipitates out of solution, leaving a colorless supernatant. These properties suggest that the protein and chromophore are covalently linked. Ion analysis revealed that the pigment does not have metal ions chelated to it. Coral pigments were also isolated from pink morphs of other pocilloporids, Seriatopora hystrix ({lambda}max = 560 nm) and Stylophora pistillata ({lambda}max = 560 nm); and from bluish regions of the acroporids, Acropora formosa (blue; {lambda}max = 590 nm) and Acropora digitifera (purple; {lambda}max = 580 nm). With the exception of A. formosa, all the corals examined had pigments with the same native (54 kD) and subunit (28 kD) molecular weights as those of P. damicornis. A. formosa pigment has a native molecular weight of about 82.6 kD and three subunits of 28 kD. The pigments isolated from each of these coral species have properties similar to those described for P. damicornis. Isolation and biochemical purification of the pigment enabled the exploration of the function of the pink pigment. Three possibilities were eliminated. The compound does not act as (i) a photoprotectant for shielding the photosynthetic pigments of symbiotic zooxanthellae against excessive

  20. CSR - A marketing tool? : A case study of ICA's and Lindex's Pink Ribbon campaign

    OpenAIRE

    Ström, Rickard; Backteman, Richard; Batmunkh, Temuulen

    2009-01-01

    Purpose: The purpose of this thesis is to investigate how a CSR strategy can be used as a marketing tool by analyzing ICA‘s and Lindex‘s marketing approach within the Pink Ribbon campaign. Background: The concept of CSR has become more and more common in business practices and customers today almost expect companies to be socially responsible. Even though CSR is very important for companies, it has historically not been a very lucrative approach for them to involve in these activities. Howeve...

  1. Oxidative stability of pork emulsion containing tomato products and pink guava pulp during refrigerated aerobic storage

    OpenAIRE

    Joseph, Serlene; Chatli, Manish K.; Biswas, Ashim K.; Sahoo, Jhari

    2012-01-01

    Lipid oxidation-induced quality problems can be minimized with the use of natural antioxidants. Antioxidant potential of tomato puree (10 %; T-1), tomato pulp (12.5 %; T-2), lyophilized tomato peel (6 %; T-3), and pink guava pulp (10 %; T-4) was evaluated in raw pork emulsion during refrigerated storage for 9 days under aerobic packaging. The lycopene and β-carotene content varied in pork emulsion as T-3 > T-1 > T-2 > T-4 and decreased (P 

  2. Hunting for the optimal hunt - Contributions to a sustainable harvest strategy for pink-footed geese

    DEFF Research Database (Denmark)

    Jensen, Gitte Høj

    As part of the recently endorsed African-Eurasian Migratory Waterbird (AEWA) International Species Management Plan for the Svalbard population of the pink-footed goose Anser brachyrhynchus, a stable population target of 60,000 (current population is c. 80,000 during 2011-2013) has been agreed...... the development of the AHM plan. This has been done at the flyway level by developing demographic population models and exploring the application of dynamic optimization methods to find an optimal management strategy. At the local and regional levels I explored effects of hunting practises and organisation at one...

  3. Isolation of an unidentified pink-pigmented bacterium in a clinical specimen.

    OpenAIRE

    Odugbemi, T; Nwofor, C; Joiner, K T

    1988-01-01

    An unidentified pink-pigmented bacterium isolated from a clinical specimen is reported. The organism was oxidase, urease, and catalase positive; it grew on Thayer-Martin and MacConkey media. The isolate is possibly similar to an unnamed taxon (G.L. Gilardi and Y.C. Faur, J. Clin. Microbiol. 20:626-629, 1984); however, it had unique characteristics of nonmotility with no flagellum detectable and was a gram-negative coccoid with a few rods in pairs and negative for starch hydrolysis.

  4. Isolation of an unidentified pink-pigmented bacterium in a clinical specimen.

    Science.gov (United States)

    Odugbemi, T; Nwofor, C; Joiner, K T

    1988-05-01

    An unidentified pink-pigmented bacterium isolated from a clinical specimen is reported. The organism was oxidase, urease, and catalase positive; it grew on Thayer-Martin and MacConkey media. The isolate is possibly similar to an unnamed taxon (G.L. Gilardi and Y.C. Faur, J. Clin. Microbiol. 20:626-629, 1984); however, it had unique characteristics of nonmotility with no flagellum detectable and was a gram-negative coccoid with a few rods in pairs and negative for starch hydrolysis.

  5. Wearing pink as a stand against bullying: why we need to say more.

    Science.gov (United States)

    Naugler, Diane

    2010-01-01

    This article presents a contextual discourse analysis of the media response to a campaign against bullying that was developed in the spring of 2007 in the Canadian province of Nova Scotia. As a feature of masculine socialization, male-on-male bullying secures the reproduction of an aggressive and heteronormative hegemonic masculinity (Connell, 1987) for boys and young men in contemporary North American mainstream culture. I argue that the celebration of the "Pink Campaign" is illustrative of the normalizing silences, or "unremarkability," about the related discourses of sexism and homophobia that motivate everyday practices of male-on-male bullying.

  6. [Isolation and preliminary characterization of carotenoids from pink-pigmented methylotrophs].

    Science.gov (United States)

    Konovalova, A M; Shylin, S O; Rokytko, P V

    2006-01-01

    An effective method was developed for complete removal of pigments from the cells and solvent mixture for further separation of pigments using thin layer chromatography on silica gel. Carotenoid samples that have been obtained in this way are of good purity for further investigations. Carotenoid pigments of pink-pigmented facultative methylotrophic bacteria Methylobacterium have been characterized. These carotenoids are represented mainly by xanthophylls, particularly hydroxycarotenoids. Strains M. fujisawaense B-3365 and M. mesophilicum B-3352 also have nonpolar carotenes in a small amount. Physico-chemical properties of carotenoids have been studied.

  7. Fitness cost of resistance to Bt cotton linked with increased gossypol content in pink bollworm larvae.

    Directory of Open Access Journals (Sweden)

    Jennifer L Williams

    Full Text Available Fitness costs of resistance to Bacillus thuringiensis (Bt crops occur in the absence of Bt toxins, when individuals with resistance alleles are less fit than individuals without resistance alleles. As costs of Bt resistance are common, refuges of non-Bt host plants can delay resistance not only by providing susceptible individuals to mate with resistant individuals, but also by selecting against resistance. Because costs typically vary across host plants, refuges with host plants that magnify costs or make them less recessive could enhance resistance management. Limited understanding of the physiological mechanisms causing fitness costs, however, hampers attempts to increase costs. In several major cotton pests including pink bollworm (Pectinophora gossypiella, resistance to Cry1Ac cotton is associated with mutations altering cadherin proteins that bind this toxin in susceptible larvae. Here we report that the concentration of gossypol, a cotton defensive chemical, was higher in pink bollworm larvae with cadherin resistance alleles than in larvae lacking such alleles. Adding gossypol to the larval diet decreased larval weight and survival, and increased the fitness cost affecting larval growth, but not survival. Across cadherin genotypes, the cost affecting larval growth increased as the gossypol concentration of larvae increased. These results suggest that increased accumulation of plant defensive chemicals may contribute to fitness costs associated with resistance to Bt toxins.

  8. Pink1 and Parkin regulate Drosophila intestinal stem cell proliferation during stress and aging.

    Science.gov (United States)

    Koehler, Christopher L; Perkins, Guy A; Ellisman, Mark H; Jones, D Leanne

    2017-08-07

    Intestinal stem cells (ISCs) maintain the midgut epithelium in Drosophila melanogaster Proper cellular turnover and tissue function rely on tightly regulated rates of ISC division and appropriate differentiation of daughter cells. However, aging and epithelial injury cause elevated ISC proliferation and decreased capacity for terminal differentiation of daughter enteroblasts (EBs). The mechanisms causing functional decline of stem cells with age remain elusive; however, recent findings suggest that stem cell metabolism plays an important role in the regulation of stem cell activity. Here, we investigate how alterations in mitochondrial homeostasis modulate stem cell behavior in vivo via RNA interference-mediated knockdown of factors involved in mitochondrial dynamics. ISC/EB-specific knockdown of the mitophagy-related genes Pink1 or Parkin suppresses the age-related loss of tissue homeostasis, despite dramatic changes in mitochondrial ultrastructure and mitochondrial damage in ISCs/EBs. Maintenance of tissue homeostasis upon reduction of Pink1 or Parkin appears to result from reduction of age- and stress-induced ISC proliferation, in part, through induction of ISC senescence. Our results indicate an uncoupling of cellular, tissue, and organismal aging through inhibition of ISC proliferation and provide insight into strategies used by stem cells to maintain tissue homeostasis despite severe damage to organelles. © 2017 Koehler et al.

  9. Logistic growth models of China pinks, cultivated on seven substrates, as a function of degree days

    Directory of Open Access Journals (Sweden)

    Marília Milani

    Full Text Available ABSTRACT: The objective of this study was to characterize the height (H and leaf number (LN of China pinks, grown in seven substrates, as a function of degree days, using the logistic growth model. H and LN were measured from 56 plants per substrate, for 392 plants in total. Plants that were grown on substrates formed of 50% soil with 50% rice husk ash (50% S + 50% RH and 80% rice husk ash with 20% worm castings (80% RH + 20% W had the longest vegetative growth period (74d, corresponding to 1317.9ºCd. The logistic growth model, adjusted for H, showed differences in the estimation of maximum expected height (α between the substrates, with values between 10.47cm for 50% S + 50% RH and 35.75cm for Mecplant(r. When α was estimated as LN, variation was also observed between the different substrates, from approximately 30 leaves on plants growing on 50% S + 50% RH to 34 leaves on the plants growing on the substrate formed of 80% RH + 20% W. Growth of China pinks can be characterized using H or LN in the logistic growth model as a function of degree days, being the provided plants adequately fertilized. The best substrates in terms of maximum height and leaf number were 80% soil + 20% worm castings and Mecplant(r. However, users must recalibrate the model with the estimated parameters before applying it to different growing conditions.

  10. Maturity stages of Pink Shrimp Farfantepenaeus notialis (Penaeidae) in the Colombian Caribbean

    International Nuclear Information System (INIS)

    Perez Ferro, Daniel Giovanny; Paramo Granados, Jorge Enrique

    2014-01-01

    The pink Shrimp Farfantepenaeus notialis is one resource of great socioeconomic importance in the Caribbean region. However, this species has been overexploited in the last decades and there is a lack of biological and fishery information. For such reason, the objective of the present study is to determine and characterize the maturity stages a macroscopic and microscopic level of the pink Shrimp (F. notialis) as input for its management. The samples were taking from June of 2012 and May 2013, on board shrimp commercial vessels in the Colombian Caribbean. The females of F. notialis were identified and preserved for the histologic analysis and some gonads were fixed. Data of size, weight and sex were taken. The gonadal stages were determined from the morphology and coloration of gonad and to microscopic determination were carried out histologic cut from samples of thirty gonads from all stages. A total of 3019 females were obtained from F. notialis, to which five stages of gonadal development were found and described. The macroscopic results were corroborated with the analysis ovocyte development a microscopic level. The development of ovocyte was coherent to that reported for F. brevirostris in the Colombian pacific and F. paulensis in the north coast of Brazil. These results are a contribution of the great scientific importance to the determination of maturity of F. notialis in the Colombian Caribbean.

  11. Distribution of pink-pigmented facultative methylotrophs on leaves of vegetables.

    Science.gov (United States)

    Mizuno, Masayuki; Yurimoto, Hiroya; Yoshida, Naoko; Iguchi, Hiroyuki; Sakai, Yasuyoshi

    2012-01-01

    The distribution of pink-pigmented facultative methylotrophs (PPFMs) on the leaves of various vegetables was studied. All kinds of vegetable leaves tested gave pink-pigmented colonies on agar plates containing methanol as sole carbon source. The numbers of PPFMs on the leaves, colony-forming units (CFU)/g of fresh leaves, differed among the plants, although they were planted and grown at the same farm. Commercial green perilla, Perilla frutescens viridis (Makino) Makino, gave the highest counts of PPFMs (2.0-4.1×10(7) CFU/g) of all the commercial vegetable leaves tested, amounting to 15% of total microbes on the leaves. The PPFMs isolated from seeds of two varieties of perilla, the red and green varieties, exhibited high sequence similarity as to the 16S rRNA gene to two different Methylobacterium species, M. fujisawaense DSM5686(T) and M. radiotolerans JCM2831(T) respectively, suggesting that there is specific interaction between perilla and the PPFMs.

  12. Behavioural response of pink salmon Fry Oncorhynchus gorbuscha to fresh, weathered, and dispersed crude oil

    International Nuclear Information System (INIS)

    Moles, A.

    2009-01-01

    Very little is known about fish behaviour in relation to oil spill contamination. In this study, out-migrant pink salmon fry Oncorhynchus gorbuscha were subjected to varying concentrations of oil and sea water solutions, or uncontaminated sea water at a temperature of 7 degrees C. Fresh oil, artificially weathered oil, and dispersed oil samples were used. Twenty trials were run for concentrations of 48 aromatic and 30alkane hydrocarbons. The number of minutes spent in the uncontaminated water was significantly higher than sample areas. Fry avoided water-accommodated mixtures of Alaska North Slope crude oil in sea water at concentrations of 960 mg/L total aromatic hydrocarbon fresh oil and 873 mg/L total aromatic hydrocarbon dispersed oil. Concentrations corresponded to 75 per cent and 68 per cent of the median lethal concentration level. However, the fry did not avoid the weathered oil, which indicated that the fry may be sensitive to monoaromatic hydrocarbon components in the oil. It was concluded that oil pollution may alter pink salmon migration behaviour. 28 refs., 1 tab., 2 figs

  13. Effects of Different Drying Conditions on Key Quality Parameters of Pink Peppercorns (Schinus terebinthifolius Raddi

    Directory of Open Access Journals (Sweden)

    Bruno Guzzo Silva

    2017-01-01

    Full Text Available Pink peppercorns are among the most sophisticated condiments in the international cuisine. This culinary spice is obtained from dried fruits of Schinus terebinthifolius Raddi, a species native to South America. In this work, a methodology for the assessment of pink peppercorn quality under various drying conditions was defined. Experiments were performed in a pilot tray dryer, which ensured integrity of the product. A central composite rotatable design with 11 experiments was devised to study the influence of drying air temperature (35–75°C and air velocity (0.3–0.9 m/s on product quality, assessed by moisture content, color (CIELAB system, and volatile compounds. The essential oils of fresh and dried fruits were extracted by hydrodistillation and analyzed by gas chromatography coupled to mass spectrometry. Air temperature had the greatest influence on the quality parameters under study, while air velocity had no statistically significant effect. Considering all quality criteria, temperatures between 40 and 55°C provided the best compromise, yielding an adequate moisture content in the dried product without dramatic degradation of color and essential oil.

  14. Pink adhesive substance on washstand, toilet and bathroom; Yokushitau toire senmendai nadoni hasseisuru pinkuiro fuchakubutsu

    Energy Technology Data Exchange (ETDEWEB)

    Furuhata, K. [The Tokyo Metropolitan Research Laboratory of Public Health, Tokyo (Japan)

    1996-01-05

    This paper describes the result of discussions on pink substances deposited on bathrooms and washstands. These deposits are in the form of cluster of rod-shaped germ-like microorganisms of various sizes, considered a kind of slime. According to a germ growing test, the number of germ was estimated to be 220,000 to 300 million per cm{sup 3}. In a test on four cases using Gram stain, the types of germs constituting the slime were found being consisted of three to seven genera, and methylobacterium is the constitutive bacteria common to the slimes. The pink color in the slimes is thought attributable to this bacteria. The slime is formed from the deposited bacteria that proliferate under high temperature and humidity using soap and other materials as its nutrient. The slime is generated in places where tap water is used. Methylobacterium has high chlorine resistivity, which gives a high possibility of the bacteria growing to a type that involves in the initial formation of the slime. Although this slime gives no effect to healthy people, infants who have been infected with specific bacteria may have their diseases aggravated as a result of activities of the slime. Slime generation may be prevented effectively by good ventilation and removal of organic matters. 4 refs., 4 figs., 4 tabs.

  15. PINK1/Parkin-Dependent Mitochondrial Surveillance: From Pleiotropy to Parkinson's Disease

    Directory of Open Access Journals (Sweden)

    Olga Corti

    2017-05-01

    Full Text Available Parkinson's disease (PD is one of the most frequent neurodegenerative disease caused by the preferential, progressive degeneration of the dopaminergic (DA neurons of the substantia nigra (SN pars compacta. PD is characterized by a multifaceted pathological process involving protein misfolding, mitochondrial dysfunction, neuroinflammation and metabolism deregulation. The molecular mechanisms governing the complex interplay between the different facets of this process are still unknown. PARK2/Parkin and PARK6/PINK1, two genes responsible for familial forms of PD, act as a ubiquitous core signaling pathway, coupling mitochondrial stress to mitochondrial surveillance, by regulating mitochondrial dynamics, the removal of damaged mitochondrial components by mitochondria-derived vesicles, mitophagy, and mitochondrial biogenesis. Over the last decade, PINK1/Parkin-dependent mitochondrial quality control emerged as a pleiotropic regulatory pathway. Loss of its function impinges on a number of physiological processes suspected to contribute to PD pathogenesis. Its role in the regulation of innate immunity and inflammatory processes stands out, providing compelling support to the contribution of non-cell-autonomous immune mechanisms in PD. In this review, we illustrate the central role of this multifunctional pathway at the crossroads between mitochondrial stress, neuroinflammation and metabolism. We discuss how its dysfunction may contribute to PD pathogenesis and pinpoint major unresolved questions in the field.

  16. The human PINK1 locus is regulated in vivo by a non-coding natural antisense RNA during modulation of mitochondrial function

    DEFF Research Database (Denmark)

    Scheele, Camilla; Petrovic, Natasa; Faghihi, Mohammad A

    2007-01-01

    BACKGROUND: Mutations in the PTEN induced putative kinase 1 (PINK1) are implicated in early-onset Parkinson's disease. PINK1 is expressed abundantly in mitochondria rich tissues, such as skeletal muscle, where it plays a critical role determining mitochondrial structural integrity in Drosophila. ...

  17. Role of PINK1 binding to the TOM complex and alternate intracellular membranes in recruitment and activation of the E3 ligase Parkin.

    Science.gov (United States)

    Lazarou, Michael; Jin, Seok Min; Kane, Lesley A; Youle, Richard J

    2012-02-14

    Mutations in the mitochondrial kinase PINK1 and the cytosolic E3 ligase Parkin can cause Parkinson's disease. Damaged mitochondria accumulate PINK1 on the outer membrane where, dependent on kinase activity, it recruits and activates Parkin to induce mitophagy, potentially maintaining organelle fidelity. How PINK1 recruits Parkin is unknown. We show that endogenous PINK1 forms a 700 kDa complex with the translocase of the outer membrane (TOM) selectively on depolarized mitochondria whereas PINK1 ectopically targeted to the outer membrane retains association with TOM on polarized mitochondria. Inducibly targeting PINK1 to peroxisomes or lysosomes, which lack a TOM complex, recruits Parkin and activates ubiquitin ligase activity on the respective organelles. Once there, Parkin induces organelle selective autophagy of peroxisomes but not lysosomes. We propose that the association of PINK1 with the TOM complex allows rapid reimport of PINK1 to rescue repolarized mitochondria from mitophagy, and discount mitochondrial-specific factors for Parkin translocation and activation. Copyright © 2012 Elsevier Inc. All rights reserved.

  18. Paleoclimatic and diagenetic history of the Late Quaternary sediments in a core from the southeastern Arabian Sea: Geochemical and magnetic signals

    Digital Repository Service at National Institute of Oceanography (India)

    Rao, V.P; Kessarkar, P; Thamban, M.; Patil, S.K.

    the last 35 kaBP. The delta sup(18)O values of Globigerinoides ruber are heaviest during the Last Glacial Maximum (LGM) and appear unaffected by low-saline waters transported from the Bay of Bengal by the strong northeast monsoon and West Indian coastal...

  19. Oxygen and carbon isotope analyses of a Late Quaternary core in the Zaire (Congo) fan

    International Nuclear Information System (INIS)

    Olausson, E.

    1984-01-01

    Oxygen and carbon isotope analyses have been carried out on samples from a core of the Angola Basin (6 0 50'S, 10 0 45'E, depth 2100 m). The pelagic foraminifer Globigerinoides ruber, a species with a shallow water habitat, and two benthic species Uvigerina peregrina and Bulimina aculeata have been analysed. The data are given relative to PDB. (Auth.)

  20. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Globigerinoides ruber) from a deep sea sediment core (GC-1) in the Andaman Sea show high glacial-to-Holocene 180 amplitude of 2.1% which is consistent with previously published records from this marginal basin and suggest increased ...

  1. Condições de atmosfera controlada para a maçã ?Pink Lady?

    Directory of Open Access Journals (Sweden)

    Brackmann Auri

    2005-01-01

    Full Text Available O objetivo do presente trabalho foi avaliar o efeito de diferentes temperaturas e pressões parciais de O2 e CO2 sobre a manutenção da qualidade da maçã (Malus domestica Borkh. cultivar Pink Lady durante o armazenamento em atmosfera controlada. Os tratamentos avaliados foram 1,5kPa de O2 + 1,0kPa de CO2 na temperatura de -0,5degreesC, e 1,5kPa de O2 + 1,0kPa de CO2; 1,5kPa de O2 + 2,0kPa de CO2; 1,0kPa de O2 + 1,0kPa de CO2; 1,0kPa de O2 + <0,5kPa de CO2; armazenamento refrigerado (20,8kPa de O2 + <0,5kPa de CO2 na temperatura de +0,5degreesC. As avaliações foram realizadas após nove meses de armazenamento, seguidos de sete dias de exposição dos frutos a 20degreesC. Os frutos mantidos em atmosfera controlada apresentaram menor ocorrência de podridões, maior firmeza de polpa e maior teor de sólidos solúveis totais, em comparação com os frutos conservados em armazenamento refrigerado. As melhores condições de atmosfera controlada para conservação da qualidade da maçã ?Pink Lady? foram 1,5kPa de O2 + 1,0kPa de CO2 na temperatura de -0,5degreesC, e 1,5kPa de O2 + 1,0kPa de CO2; 1,5kPa de O2 + 2,0kPa de CO2 na temperatura de +0,5degreesC, já as pressões parciais 1,0kPa de O2 + 1,0kPa de CO2 e 1,0kPa de O2 + <0,5kPa de CO2 apresentaram maiores valores de podridão e degenerescência senescente, respectivamente. A atmosfera controlada, com exceção da atmosfera refrigerada, mantém conservada a qualidade da maçã ?Pink Lady?.

  2. Presence, distribution and effect of white, pink and purple morphs on pollination in the orchid Orchis mascula

    Directory of Open Access Journals (Sweden)

    Bertrand Schatz

    2013-12-01

    Full Text Available How floral polymorphism of flowering plants can be maintained in evolutionary time has long intrigued ecologists and is still debated. In particular, how floral colour polymorphism influences reproductive success is still poorly understood. Here, we investigated the case of Orchis mascula, a deceptive orchid species in which the presence of rare white-flowered individuals is known to increase the percentage pollination of co-occurring coloured morphs. In a brief review, we report all the orchid species for which rare colour morphs are recorded and show that colour polymorphism occurs in most orchid genera occurring in France. In this study, more than 20,000 individuals of O. mascula were surveyed and some rare clear pink morphs were recorded. The frequencies of white-flowered and clear pink-flowered individuals were 0.59% and 0.28%, respectively. These two rare-colour flowered individuals were not randomly distributed and restricted to a few populations. In addition, the presence of pink-flowered individuals and the use of experimental pink lures resulted in an increase in the percentage pollination of surrounding purple-flowered individuals, as previously shown for white-flowered individuals and white lures. These new observations favour kin selection as the means by which floral colour polymorphism is maintained in this species. We suggest conducting comparative studies of other species in order to evaluate the importance of this mechanism in orchid pollination and that of other plant families.

  3. Pink Frilly Dresses and the Avoidance of All Things "Girly": Children's Appearance Rigidity and Cognitive Theories of Gender Development

    Science.gov (United States)

    Halim, May Ling; Ruble, Diane N.; Tamis-LeMonda, Catherine S.; Zosuls, Kristina M.; Lurye, Leah E.; Greulich, Faith K.

    2014-01-01

    Many young children pass through a stage of gender appearance rigidity; girls insist on wearing dresses, often pink and frilly, whereas boys refuse to wear anything with a hint of femininity. In 2 studies, we investigated the prevalence of this apparent hallmark of early gender development and its relation to children's growing identification with…

  4. Field evaluation of two systemic neonicotinoid insecticides against pink hibiscus mealybug (Maconellicoccus hirsutus (Green))on mulberry trees

    Science.gov (United States)

    Infestations of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green), in ornamental trees were already in an advanced state at the time of its discovery in the Imperial Valley of California (USA) in August 1999. Concern about the spread of M. hirsutus beyond the Imperial Valley led to the p...

  5. Temporally isolated lineages of Pink salmon reveal unique signatures of selection on distinct pools of standing genetic variation

    DEFF Research Database (Denmark)

    Limborg, Morten; Waples, R.K.; Seeb, J.E.

    2014-01-01

    A species’ genetic diversity bears the marks of evolutionary processes that have occurred throughout its history. However, robust detection of selection in wild populations is difficult and often impeded by lack of replicate tests. Here, we investigate selection in pink salmon (Oncorhynchus gorbu...

  6. E3 Ligase Subunit Fbxo15 and PINK1 Kinase Regulate Cardiolipin Synthase 1 Stability and Mitochondrial Function in Pneumonia

    Directory of Open Access Journals (Sweden)

    Bill B. Chen

    2014-04-01

    Full Text Available Acute lung injury (ALI is linked to mitochondrial injury, resulting in impaired cellular oxygen utilization; however, it is unknown how these events are linked on the molecular level. Cardiolipin, a mitochondrial-specific lipid, is generated by cardiolipin synthase (CLS1. Here, we show that S. aureus activates a ubiquitin E3 ligase component, Fbxo15, that is sufficient to mediate proteasomal degradation of CLS1 in epithelia, resulting in decreased cardiolipin availability and disrupted mitochondrial function. CLS1 is destabilized by the phosphatase and tensin homolog (PTEN-induced putative kinase 1 (PINK1, which binds CLS1 to phosphorylate and regulates CLS1 disposal. Like Fbxo15, PINK1 interacts with and regulates levels of CLS1 through a mechanism dependent upon Thr219. S. aureus infection upregulates this Fbxo15-PINK1 pathway to impair mitochondrial integrity, and Pink1 knockout mice are less prone to S. aureus-induced ALI. Thus, ALI-associated disruption of cellular bioenergetics involves bioeffectors that utilize a phosphodegron to elicit ubiquitin-mediated disposal of a key mitochondrial enzyme.

  7. Hit-size effectiveness theory applied to high doses of low LET radiation for pink mutations in Tradescantia

    International Nuclear Information System (INIS)

    Varma, M.N.; Bond, V.P.; Matthews, G.

    1985-01-01

    A hit-size effectiveness function which represents the probability of inducing a pink mutation in Tradescantia as a function of lineal energy density has been obtained (1) using observed pink mutation data for seven different radiation qualities and their respective single event microdosimetric spectra. In obtaining this function only the linear portions of dose-response curves were used. A significant improvement of the concepts embodied in the proposed hit-size effectiveness theory would be the demonstration of its applicability at high doses (where multiple hits are produced) and high dose rates (at which no significant biological repair takes place). In this article details are given on preliminary calculations of the pink mutation frequency in Tradescantia at 1, 5, 10, 20, and 60 rads for 250 kVp x rays, using the multi-hit spectra and the hit-size effectiveness function obtained on the basis of single hit microdosimetric spectra as outline in (1). A comparison of the calculated and observed pink mutation frequencies indicate excellent agreement and suggests the possibility of obtaining the hit-size effectiveness function from high dose biological-effect data obtained using low-LET radiations. 6 refs., 3 figs., 3 tabs

  8. The halo effect: suppression of pink bollworm on non-Bt cotton by Bt cotton in China.

    Directory of Open Access Journals (Sweden)

    Peng Wan

    Full Text Available In some previously reported cases, transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt have suppressed insect pests not only in fields planted with such crops, but also regionally on host plants that do not produce Bt toxins. Here we used 16 years of field data to determine if Bt cotton caused this "halo effect" against pink bollworm (Pectinophora gossypiella in six provinces of the Yangtze River Valley of China. In this region, the percentage of cotton hectares planted with Bt cotton increased from 9% in 2000 to 94% in 2009 and 2010. We found that Bt cotton significantly decreased the population density of pink bollworm on non-Bt cotton, with net decreases of 91% for eggs and 95% for larvae on non-Bt cotton after 11 years of Bt cotton use. Insecticide sprays targeting pink bollworm and cotton bollworm (Helicoverpa armigera decreased by 69%. Previously reported evidence of the early stages of evolution of pink bollworm resistance to Bt cotton in China has raised concerns that if unchecked, such resistance could eventually diminish or eliminate the benefits of Bt cotton. The results reported here suggest that it might be possible to find a percentage of Bt cotton lower than the current level that causes sufficient regional pest suppression and reduces the risk of resistance.

  9. Lunar periodicity in the shell flux of planktonic foraminifera in the Gulf of Mexico

    Science.gov (United States)

    Jonkers, Lukas; Reynolds, Caitlin E.; Richey, Julie N.; Hall, Ian R.

    2015-01-01

    Synchronised reproduction offers clear benefits to planktonic foraminifera – an important group of marine calcifiers – as it increases the chances of successful gamete fusion. Such synchrony requires tuning to an internal or external clock. Evidence exists for lunar reproductive cycles in some species, but its recognition in shell flux time series has proven difficult, raising questions about reproductive strategies. Using spectral analysis of a 4-year time series (mostly at weekly resolution) from the northern Gulf of Mexico, we show that the shell flux ofGloborotalia menardii, Globigerinella siphonifera, Orbulina universa, Globigerinoides sacculifer, Globigerinoides ruber (both pink and white varieties), Pulleniatina obliquiloculata, Neogloboquadrina dutertrei, Globigerinella calida and Globigerinita glutinata is characterised by lunar periodicity. However, the lunar rhythm is not present in all size fractions of each species and tends to be more dominant in the flux of larger shells, consistent with reproduction being more prevalent in larger specimens. Lunar periodicity is superimposed on longer term/seasonal changes in the shell fluxes, but accounts for a significant part of the variance in the fluxes. The amplitude of the lunar cycle increases roughly proportional with the magnitude of the flux, demonstrating that most of the population is indeed affected by lunar-phased synchronisation. In most species peak fluxes occur predominantly around, or just after, full moon. Only G. siphonifera and G. calida show a contrasting pattern with peaks concentrated around new moon. Although the exact cause of the synchronisation remains elusive, our data considerably increase the number of species for which lunar synchronised reproduction is reported and suggest that such reproductive behaviour is common in many species of planktonic foraminifera.

  10. What is the Right Temperature Sensitivity for Foraminiferal Mg/ca Paleothermometry in Ancient Oceans?

    Science.gov (United States)

    Eggins, S.; Holland, K.; Hoenisch, B.; Spero, H. J.; Allen, K. A.

    2013-12-01

    Mg/Ca seawater thermometry has become a cornerstone of modern paleoceanography. Laboratory experiments, seafloor core-top samples, plankton trap and tow collected materials all indicate consistent temperature sensitivity (9-10% increase in Mg/Ca per °C) for a full range of modern planktic foraminifer species. While these results demonstrate the overall robustness of Mg/Ca paleothermometry for the modern ocean, it is an empirical tool for which there is limited understanding of its bio-physio-chemical basis and its applicability to ancient oceans. We have undertaken experimental cultures of Orbulina universa, Globigerinoides sacculifer and Globigerinoides ruber (pink) across a range of seawater compositions (temperature, carbonate chemistry and Mg/Casw) that encompass modern and ancient Paleogene and Cretaceous ocean compositions (Mg/Casw 0.25x to 2x modern and pCO2 = 200 to 1500 ppmv). Our results reveal that the sensitivity of the Mg/Ca-thermometer for planktic foraminifers reduces significantly with Mg/Casw, rather than remaining constant as has been widely assumed or, increasing at lower Mg/Casw as proposed recently by Evans and Müller (2012). These results indicate that the modern sensitivity of 9-10% increase in Mg/Ca per °C cannot yet be applied to obtain reliable relative temperature change estimates to ancient oceans. These results further suggest that variations in foraminiferal Mg/Ca compositions in ancient oceans with lower Mg/Casw may correspond to larger temperature variations than in the modern ocean. Evans D. and Müller W., Paleoceanography, vol. 27, PA4205, doi:10.1029/2012PA002315, 2012

  11. Hypofolins A - L, ent-Labdane Diterpenoids from the Roots of Hypoestes phyllostachya 'Pink Splash'.

    Science.gov (United States)

    Cheng, Bin; Ding, Lin-Fen; Yan, Tong; Xie, Zhang-Qiao; Zhang, Zhi-Jun; Song, Liu-Dong; Wu, Xing-De; Zhao, Qin-Shi

    2018-06-01

    Twelve new ent-labdane diterpenoids, hypofolins A - F (1 - 6) and hypofolins G - L (7a/7b, 8a/8b, and 9a/9b), were isolated from the roots of Hypoestes phyllostachya 'Pink Splash'. Their structures were elucidated by extensive 1D- and 2D-NMR spectroscopic and HR-MS data. The absolute configurations of 1, 2, 5, and 7a/7b were determined by single crystal X-ray diffraction and ECD analysis, as well as chemical transformations. Compounds 7a/7b, 8a/8b, and 9a/9b were isolated as three pairs of interconverting mixture of two isomers between ketone and hemiketal types. Compound 1 showed weak cytotoxicity against SMMC-7721 cell line with IC 50 value of 31.40 μm. © 2018 Wiley-VHCA AG, Zurich, Switzerland.

  12. Petrographic and mineralogical features of the uraniferous pink granites in the north eastern desert of egypt

    International Nuclear Information System (INIS)

    Atawiya, M.Y.; Salman, A.B.; El-Bayyomi, R.M.

    1998-01-01

    The present work is concerned with the petrological, mineralogical and geochemical studies of some uranium bearing younger granites in the north eastern desert of egypt particularly Gebel Gattar area. The area around Gebel Gattar comprises the following rock units (starting from the oldest): meta volcanic, diorite-grano-diorite complex- Dokhan volcanics- Hammamat sediments, younger granites and dykes. The most significant structural features are represented by NNE-ENE dominantly trending faults and joints. Petrographicaly, the pink granites are divided into normal and mineralized (uraniferous) granites. Normal granites are classified into three types; a) leucocratic perthitic granite, b) hornblende- biotite perthitic granite and c) two feldspars perthitic granite. Mineralized granites are sheared, deformed, pinkish brown in colour and strongly altered. A remarkable secondary uranium mineralization has been recorded along fault and fracture zones

  13. Genome sequence of the dark pink pigmented Listia bainesii microsymbiont Methylobacterium sp. WSM2598.

    Science.gov (United States)

    Ardley, Julie; Tian, Rui; Howieson, John; Yates, Ron; Bräu, Lambert; Han, James; Lobos, Elizabeth; Huntemann, Marcel; Chen, Amy; Mavromatis, Konstantinos; Markowitz, Victor; Ivanova, Natalia; Pati, Amrita; Goodwin, Lynne; Woyke, Tanja; Kyrpides, Nikos; Reeve, Wayne

    2014-01-01

    Strains of a pink-pigmented Methylobacterium sp. are effective nitrogen- (N2) fixing microsymbionts of species of the African crotalarioid genus Listia. Strain WSM2598 is an aerobic, motile, Gram-negative, non-spore-forming rod isolated in 2002 from a Listia bainesii root nodule collected at Estcourt Research Station in South Africa. Here we describe the features of Methylobacterium sp. WSM2598, together with information and annotation of a high-quality draft genome sequence. The 7,669,765 bp draft genome is arranged in 5 scaffolds of 83 contigs, contains 7,236 protein-coding genes and 18 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 G enomic E ncyclopedia for B acteria and A rchaea- R oot N odule B acteria (GEBA-RNB) project.

  14. Foraging behaviour of pink-footed geese (Anser brachyrhynchus) during spring migration

    DEFF Research Database (Denmark)

    Chudzińska, Magda Ewa

    and their energetic consequences are therefore of great importance to these birds. In this thesis, I have aimed to address some aspects of the foraging decisions and behaviour of pink-footed geese during their spring migration to the Arctic breeding area. I combined field techniques with telemetry technology as well...... as modelling tools to address questions about how geese forage and fuel during their spring migration. The first three presented manuscripts focus on changes in goose foraging behaviour and energetics over the course of the day, a stopover season and the entire migration. They also focus on variety of factors...... the question: which foraging decision do geese make at the Mid-Norway stopover site....

  15. A System for Harvesting Eggs from the Pink-Spotted Lady Beetle

    Directory of Open Access Journals (Sweden)

    Margaret L. Allen

    2012-01-01

    Full Text Available We describe a system for harvesting eggs from a predatory insect, the pink-spotted lady beetle, Coleomegilla maculata De Geer (Coleoptera: Coccinellidae. Adult beetles placed in square, transparent containers that included oviposition substrates hanging from the top of the cage deposited eggs on the materials provided. We harvested eggs from these substrates in quantities sufficient for either destructive sampling or synchronous development of larvae. We evaluated effects of crowding inside cages; effects of a chemical attractant on oviposition behavior; egg cannibalism. Females preferred a textured surface rather than a smooth, waxy one for laying eggs. Crowding inhibited oviposition of beetles. Presence of a chemical attractant (methyl salicylate did not significantly improve oviposition. This paper describes an inexpensive system for harvesting eggs from C. maculata. Refinement of this system should improve oviposition and reduce cannibalism.

  16. Phosphorylation by PINK1 releases the UBL domain and initializes the conformational opening of the E3 ubiquitin ligase Parkin.

    Directory of Open Access Journals (Sweden)

    Thomas R Caulfield

    2014-11-01

    Full Text Available Loss-of-function mutations in PINK1 or PARKIN are the most common causes of autosomal recessive Parkinson's disease. Both gene products, the Ser/Thr kinase PINK1 and the E3 Ubiquitin ligase Parkin, functionally cooperate in a mitochondrial quality control pathway. Upon stress, PINK1 activates Parkin and enables its translocation to and ubiquitination of damaged mitochondria to facilitate their clearance from the cell. Though PINK1-dependent phosphorylation of Ser65 is an important initial step, the molecular mechanisms underlying the activation of Parkin's enzymatic functions remain unclear. Using molecular modeling, we generated a complete structural model of human Parkin at all atom resolution. At steady state, the Ub ligase is maintained inactive in a closed, auto-inhibited conformation that results from intra-molecular interactions. Evidently, Parkin has to undergo major structural rearrangements in order to unleash its catalytic activity. As a spark, we have modeled PINK1-dependent Ser65 phosphorylation in silico and provide the first molecular dynamics simulation of Parkin conformations along a sequential unfolding pathway that could release its intertwined domains and enable its catalytic activity. We combined free (unbiased molecular dynamics simulation, Monte Carlo algorithms, and minimal-biasing methods with cell-based high content imaging and biochemical assays. Phosphorylation of Ser65 results in widening of a newly defined cleft and dissociation of the regulatory N-terminal UBL domain. This motion propagates through further opening conformations that allow binding of an Ub-loaded E2 co-enzyme. Subsequent spatial reorientation of the catalytic centers of both enzymes might facilitate the transfer of the Ub moiety to charge Parkin. Our structure-function study provides the basis to elucidate regulatory mechanisms and activity of the neuroprotective Parkin. This may open up new avenues for the development of small molecule Parkin

  17. Pink bollworm integrated management using sterile insects under field trial conditions, Imperial Valley, California

    International Nuclear Information System (INIS)

    Walters, M.L.; Staten, R.T.; Roberson, R.C.

    2000-01-01

    The pink bollworm moth (Pectinophora gossypiella Saunders) feeds almost exclusively on cotton (Gossypium spp.) and causes economic loss (Pfadt 1978). The pink bollworm (PBW) is often the key pest of cotton in Arizona, southern California, and northwestern Mexico. The larvae (immature stages) bore into the developing cotton fruit, where they feed on the cotton lint and seeds, causing significant damage and dramatically reducing the yield of cotton lint (Pfadt 1978). The PBW is difficult to control with conventional means (insecticides) because it spends the destructive larval phase inside the cotton boll where it is well protected from control measures. Cultural controls, such as a short growing season, have successfully decreased the population in the Imperial Valley (Chu et al. 1992) to the point where eradication may be possible using sterile insects and genetically engineered cotton. Because the PBW is an introduced insect, with few plant hosts other than cultivated cotton, its eradication from continental USA is a desirable and economically attractive alternative to the continued use of pesticides and/or further loss to the pest. Mass releases of sterile insects began in earnest in 1970 in the San Joaquin Valley, California, in order to inhibit normal reproduction and to eradicate the pest in an environmentally responsible manner. Sterile release involves mass production and sexual sterilisation using irradiation (20 krad for PBW adults). This was accomplished by building a rearing facility in Phoenix, AZ. The facility has 6,410 square metres of permanent laboratories, rearing and irradiation chambers and insect packing rooms. The facility operates the year round but with a variable production rate, that is, maximal during the cotton growing season (May through September). Sterile insect technology is based on the monitoring of the native and sterile populations in the field and the subsequent release of appropriate numbers of sterile insects in order to

  18. Evidence of damage to pink salmon inhabiting Prince William Sound, Alaska, three generations after the Exxon Valdez oil spill

    International Nuclear Information System (INIS)

    Bue, B.G.; Miller, G.D.; Seeb, J.E.; Sharr, S.

    1995-01-01

    Investigations into the environmental effects of the 1 989 Exxon Valdez oil spill lead us to conclude that chronic damage occurred in some pink salmon populations. Differences in survival between streams contaminated by oil and uncontaminated streams have been observed annually since the spill for pink salmon embryos incubating in the intertidal portions of Prince William Sound. The authors assessed the environmental influence on these findings by collecting gametes from both contaminated and uncontaminated streams, transporting them to a hatchery where intra-stream crosses were made, and incubating the resulting embryos under identical conditions. Lower survival was detected in the embryos originating from the oil-contaminated streams indicating that the agent responsible for the differences detected in the field was genetic rather than environmental

  19. Degradation mechanism of Direct Pink 12B treated by iron-carbon micro-electrolysis and Fenton reaction.

    Science.gov (United States)

    Wang, Xiquan; Gong, Xiaokang; Zhang, Qiuxia; Du, Haijuan

    2013-12-01

    The Direct Pink 12B dye was treated by iron-carbon micro-electrolysis (ICME) and Fenton oxidation. The degradation pathway of Direct Pink 12B dye was inferred by ultraviolet visible (UV-Vis), infrared absorption spectrum (IR) and high performance liquid chromatography-mass spectrometry (HPLC-MS). The major reason of decolorization was that the conjugate structure was disrupted in the iron-carbon micro-electrolysis (ICME) process. However, the dye was not degraded completely because benzene rings and naphthalene rings were not broken. In the Fenton oxidation process, the azo bond groups surrounded by higher electron cloud density were first attacked by hydroxyl radicals to decolorize the dye molecule. Finally benzene rings and naphthalene rings were mineralized to H2O and CO2 under the oxidation of hydroxyl radicals. Copyright © 2013 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.

  20. Carotenoid Profile, Antioxidant Capacity, and Chromoplasts of Pink Guava (Psidium guajava L. Cv. 'Criolla') during Fruit Ripening.

    Science.gov (United States)

    Rojas-Garbanzo, Carolina; Gleichenhagen, Maike; Heller, Annerose; Esquivel, Patricia; Schulze-Kaysers, Nadine; Schieber, Andreas

    2017-05-10

    Pigments of pericarp and pulp of pink guava (Psidium guajava L. cv. 'Criolla') were investigated to elucidate the profile and the accumulation of main carotenoids during four stages of fruit ripening by using HPLC-DAD and APCI-MS/MS analysis. Seventeen carotenoids were identified, and changes in their profile during fruit ripening were observed. The carotenoids all-trans-β-carotene, 15-cis-lycopene, and all-trans-lycopene were present in all ripening stages, but all-trans-lycopene was found to be predominant (from 63% to 92% of total carotenoids) and responsible for the high lipophilic antioxidant capacity determined by spectrophotometric assays. By using light and transmission electron microscopy, the development of chromoplasts in pericarp and pulp was demonstrated. The accumulation of all-trans-lycopene and all-trans-β-carotene coincided with the development of large crystals; the chromoplasts of pink guava belong, therefore, to the crystalline type.

  1. PKA Phosphorylation of NCLX Reverses Mitochondrial Calcium Overload and Depolarization, Promoting Survival of PINK1-Deficient Dopaminergic Neurons

    Directory of Open Access Journals (Sweden)

    Marko Kostic

    2015-10-01

    Full Text Available Mitochondrial Ca2+ overload is a critical, preceding event in neuronal damage encountered during neurodegenerative and ischemic insults. We found that loss of PTEN-induced putative kinase 1 (PINK1 function, implicated in Parkinson disease, inhibits the mitochondrial Na+/Ca2+ exchanger (NCLX, leading to impaired mitochondrial Ca2+ extrusion. NCLX activity was, however, fully rescued by activation of the protein kinase A (PKA pathway. We further show that PKA rescues NCLX activity by phosphorylating serine 258, a putative regulatory NCLX site. Remarkably, a constitutively active phosphomimetic mutant of NCLX (NCLXS258D prevents mitochondrial Ca2+ overload and mitochondrial depolarization in PINK1 knockout neurons, thereby enhancing neuronal survival. Our results identify an mitochondrial Ca2+ transport regulatory pathway that protects against mitochondrial Ca2+ overload. Because mitochondrial Ca2+ dyshomeostasis is a prominent feature of multiple disorders, the link between NCLX and PKA may offer a therapeutic target.

  2. Mucuna pruriens (Velvet bean) rescues motor, olfactory, mitochondrial and synaptic impairment in PINK1B9 Drosophila melanogaster genetic model of Parkinson's disease.

    Science.gov (United States)

    Poddighe, Simone; De Rose, Francescaelena; Marotta, Roberto; Ruffilli, Roberta; Fanti, Maura; Secci, Pietro Paolo; Mostallino, Maria Cristina; Setzu, Maria Dolores; Zuncheddu, Maria Antonietta; Collu, Ignazio; Solla, Paolo; Marrosu, Francesco; Kasture, Sanjay; Acquas, Elio; Liscia, Anna

    2014-01-01

    The fruit fly Drosophila melanogaster (Dm) mutant for PTEN-induced putative kinase 1 (PINK1B9) gene is a powerful tool to investigate physiopathology of Parkinson's disease (PD). Using PINK1B9 mutant Dm we sought to explore the effects of Mucuna pruriens methanolic extract (Mpe), a L-Dopa-containing herbal remedy of PD. The effects of Mpe on PINK1B9 mutants, supplied with standard diet to larvae and adults, were assayed on 3-6 (I), 10-15 (II) and 20-25 (III) days old flies. Mpe 0.1% significantly extended lifespan of PINK1B9 and fully rescued olfactory response to 1-hexanol and improved climbing behavior of PINK1B9 of all ages; in contrast, L-Dopa (0.01%, percentage at which it is present in Mpe 0.1%) ameliorated climbing of only PINK1B9 flies of age step II. Transmission electron microscopy analysis of antennal lobes and thoracic ganglia of PINK1B9 revealed that Mpe restored to wild type (WT) levels both T-bars and damaged mitochondria. Western blot analysis of whole brain showed that Mpe, but not L-Dopa on its own, restored bruchpilot (BRP) and tyrosine hydroxylase (TH) expression to age-matched WT control levels. These results highlight multiple sites of action of Mpe, suggesting that its effects cannot only depend upon its L-Dopa content and support the clinical observation of Mpe as an effective medication with intrinsic ability of delaying the onset of chronic L-Dopa-induced long-term motor complications. Overall, this study strengthens the relevance of using PINK1B9 Dm as a translational model to study the properties of Mucuna pruriens for PD treatment.

  3. Mucuna pruriens (Velvet bean) Rescues Motor, Olfactory, Mitochondrial and Synaptic Impairment in PINK1B9 Drosophila melanogaster Genetic Model of Parkinson’s Disease

    Science.gov (United States)

    Ruffilli, Roberta; Fanti, Maura; Secci, Pietro Paolo; Mostallino, Maria Cristina; Setzu, Maria Dolores; Zuncheddu, Maria Antonietta; Collu, Ignazio; Solla, Paolo; Marrosu, Francesco; Kasture, Sanjay; Acquas, Elio; Liscia, Anna

    2014-01-01

    The fruit fly Drosophila melanogaster (Dm) mutant for PTEN-induced putative kinase 1 (PINK1B9) gene is a powerful tool to investigate physiopathology of Parkinson's disease (PD). Using PINK1B9 mutant Dm we sought to explore the effects of Mucuna pruriens methanolic extract (Mpe), a L-Dopa-containing herbal remedy of PD. The effects of Mpe on PINK1B9 mutants, supplied with standard diet to larvae and adults, were assayed on 3–6 (I), 10–15 (II) and 20–25 (III) days old flies. Mpe 0.1% significantly extended lifespan of PINK1B9 and fully rescued olfactory response to 1-hexanol and improved climbing behavior of PINK1B9 of all ages; in contrast, L-Dopa (0.01%, percentage at which it is present in Mpe 0.1%) ameliorated climbing of only PINK1B9 flies of age step II. Transmission electron microscopy analysis of antennal lobes and thoracic ganglia of PINK1B9 revealed that Mpe restored to wild type (WT) levels both T-bars and damaged mitochondria. Western blot analysis of whole brain showed that Mpe, but not L-Dopa on its own, restored bruchpilot (BRP) and tyrosine hydroxylase (TH) expression to age-matched WT control levels. These results highlight multiple sites of action of Mpe, suggesting that its effects cannot only depend upon its L-Dopa content and support the clinical observation of Mpe as an effective medication with intrinsic ability of delaying the onset of chronic L-Dopa-induced long-term motor complications. Overall, this study strengthens the relevance of using PINK1B9 Dm as a translational model to study the properties of Mucuna pruriens for PD treatment. PMID:25340511

  4. Mucuna pruriens (Velvet bean rescues motor, olfactory, mitochondrial and synaptic impairment in PINK1B9 Drosophila melanogaster genetic model of Parkinson's disease.

    Directory of Open Access Journals (Sweden)

    Simone Poddighe

    Full Text Available The fruit fly Drosophila melanogaster (Dm mutant for PTEN-induced putative kinase 1 (PINK1B9 gene is a powerful tool to investigate physiopathology of Parkinson's disease (PD. Using PINK1B9 mutant Dm we sought to explore the effects of Mucuna pruriens methanolic extract (Mpe, a L-Dopa-containing herbal remedy of PD. The effects of Mpe on PINK1B9 mutants, supplied with standard diet to larvae and adults, were assayed on 3-6 (I, 10-15 (II and 20-25 (III days old flies. Mpe 0.1% significantly extended lifespan of PINK1B9 and fully rescued olfactory response to 1-hexanol and improved climbing behavior of PINK1B9 of all ages; in contrast, L-Dopa (0.01%, percentage at which it is present in Mpe 0.1% ameliorated climbing of only PINK1B9 flies of age step II. Transmission electron microscopy analysis of antennal lobes and thoracic ganglia of PINK1B9 revealed that Mpe restored to wild type (WT levels both T-bars and damaged mitochondria. Western blot analysis of whole brain showed that Mpe, but not L-Dopa on its own, restored bruchpilot (BRP and tyrosine hydroxylase (TH expression to age-matched WT control levels. These results highlight multiple sites of action of Mpe, suggesting that its effects cannot only depend upon its L-Dopa content and support the clinical observation of Mpe as an effective medication with intrinsic ability of delaying the onset of chronic L-Dopa-induced long-term motor complications. Overall, this study strengthens the relevance of using PINK1B9 Dm as a translational model to study the properties of Mucuna pruriens for PD treatment.

  5. Control of Wilt and Rot Pathogens of Tomato by Antagonistic Pink Pigmented Facultative Methylotrophic Delftia lacustris and Bacillus spp.

    OpenAIRE

    Janahiraman, Veeranan; Anandham, Rangasamy; Kwon, Soon W.; Sundaram, Subbiah; Karthik Pandi, Veeranan; Krishnamoorthy, Ramasamy; Kim, Kiyoon; Samaddar, Sandipan; Sa, Tongmin

    2016-01-01

    The studies on the biocontrol potential of pink pigmented facultative methylotrophic (PPFM) bacteria other than the genus Methylobacterium are scarce. In the present study, we report three facultative methylotrophic isolates; PPO-1, PPT-1 and PPB-1, respectively identified as Delftia lacustris, Bacillus subtilis and Bacillus cereus by 16S rRNA gene sequence analysis. Hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicate...

  6. Numerical study of hydrodynamic and salinity transport processes in the Pink Beach wetlands of the Liao River estuary, China

    Directory of Open Access Journals (Sweden)

    H. Qiao

    2018-06-01

    Full Text Available Interaction studies of vegetation within flow environments are essential for the determination of bank protection, morphological characteristics and ecological conditions for wetlands. This paper uses the MIKE 21 hydrodynamic and salinity model to simulate the hydrodynamic characteristics and salinity transport processes in the Pink Beach wetlands of the Liao River estuary. The effect of wetland plants on tidal flow in wetland areas is represented by a varying Manning coefficient in the bottom friction term. Acquisition of the vegetation distribution is based on Landsat TM satellites by remote sensing techniques. Detailed comparisons between field observation and simulated results of water depth, salinity and tidal currents are presented in the vegetated domain of the Pink Beach wetlands. Satisfactory results were obtained from simulations of both flow characteristics and salinity concentration, with or without vegetation. A numerical experiment was conducted based on variations in vegetation density, and compared with the tidal currents in non-vegetated areas; the computed current speed decreased remarkably with an increase in vegetation density. The impact of vegetation on water depth and salinity was simulated, and the findings revealed that wetland vegetation has an insignificant effect on the water depth and salinity in this wetland domain. Several stations (from upstream to downstream in the Pink Beach wetlands were selected to estimate the longitudinal variation of salinity under different river runoff conditions; the results showed that salinity concentration decreases with an increase in river runoff. This study can consequently help increase the understanding of favourable salinity conditions for particular vegetation growth in the Pink Beach wetlands of the Liao River estuary. The results also provide crucial guidance for related interaction studies of vegetation, flow and salinity in other wetland systems.

  7. Pink Mold Rot on Unishiu Orange (Citrus unshiu Mac. Caused by Trichothecium roseum (Pers. Link ex Gray in Korea

    Directory of Open Access Journals (Sweden)

    Jin-Hyeuk Kwon

    2013-09-01

    Full Text Available In 2012, a pink mold rot was observed on unishiu orange (Citrus unshiu Mac. fruits at the Wholesale Market for Agricultural Products, Jinju, Korea. The symptom on unishiu orange was a water-soaked lesion on the surface of fruit, which later on enlarged to form softened brown rot lesions. The diseased fruits were covered with pink-colored mold, consisting of conidia and conidiophores of the pathogen. Optimum temperature for mycelial growth was 25oC. Conidia were hyaline, smooth, 2-celled, and thick-walled conidia with truncate bases, ellipsoidal to pyriform, characteristically held together zig-zag chains and 12−26 × 8−12 μm in size. Conidiophore was erect, colorless, unbranched, and 4−5 μm wide. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete ITS rDNA region, the causal fungus was identified as Trichothecium roseum (Pers. Link ex Gray. This is the first report of pink mold rot caused by T. roseum on unishiu orange in Korea.

  8. Effects of oil-contaminated prey on the feeding, growth, and related energetics on pink salmon, Oncorhynchus gorbuscha Walbaum, fry

    International Nuclear Information System (INIS)

    Schwartz, J.P.

    1984-01-01

    Pink salmon, Oncorhynchus gorbuscha Walbaum, fry were exposed to oil contaminated prey (OCP) in a series of experiments to determine the effect of oil exposure via the diet on the ability of pink fry to survive. Brine shrimp, Artemia salina, nauplii were contaminated with petroleum hydrocarbons by exposure to the water-soluble fraction (WSF) of Cook Inlet crude oil and fed to the fish. Feeding rates were measured for 10 days using OCP and for 5 days using uncontaminated prey (post-exposure period). In a separate experiment, fry growth was measured over a 50 day period. In another experiment, fry oxygen consumption, food absorption and utilization, and ammonia excretion was measured to determine the effects of OCP on fry metabolic activity. Results indicate that exposure to OCP can reduce fry growth primarily by reducing food intake, but additional nutrition is lost from the non-absorption of ingested food. Reductions in growth could decrease fry survival, and thereby reduce the number of returning adult pink salmon

  9. Genetic Analysis of PARK2 and PINK1 Genes in Brazilian Patients with Early-Onset Parkinson's Disease

    Directory of Open Access Journals (Sweden)

    Karla Cristina Vasconcelos Moura

    2013-01-01

    Full Text Available Parkinson's disease is the second most frequent neurodegenerative disorder in the world, affecting 1-2% of individuals over the age of 65. The etiology of Parkinson's disease is complex, with the involvement of gene-environment interactions. Although it is considered a disease of late manifestation, early-onset forms of parkinsonism contribute to 5–10% of all cases. In the present study, we screened mutations in coding regions of PARK2 and PINK1 genes in 136 unrelated Brazilian patients with early-onset Parkinson's disease through automatic sequencing. We identified six missense variants in PARK2 gene: one known pathogenic mutation, two variants of uncertain role, and three nonpathogenic changes. No pathogenic mutation was identified in PINK1 gene, only benign polymorphisms. All putative pathogenic variants found in this study were in heterozygous state. Our data show that PARK2 point mutations are more common in Brazilian early-onset Parkinson's disease patients (2.9% than PINK1 missense variants (0%, corroborating other studies worldwide.

  10. Evaluation of intraspecies interactions in biofilm formation by Methylobacterium species isolated from pink-pigmented household biofilms.

    Science.gov (United States)

    Xu, Fang-Fang; Morohoshi, Tomohiro; Wang, Wen-Zhao; Yamaguchi, Yuka; Liang, Yan; Ikeda, Tsukasa

    2014-01-01

    Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mechanisms underlying biofilm formation by using the bacterial consortium found in household pink slime. A clone library analysis revealed that Methylobacterium was the predominant genus in household pink slime. In addition, 16 out of 21 pink-pigmented bacterial isolates were assigned to the genus Methylobacterium. Although all of the Methylobacterium isolates formed low-level biofilms, the amount of the biofilms formed by Methylobacterium sp. P-1M and P-18S was significantly increased by co-culturing with other Methylobacterium strains that belonged to a specific phylogenetic group. The single-species biofilm was easily washed from the glass surface, whereas the dual-species biofilm strongly adhered after washing. A confocal laser scanning microscopy analysis showed that the dual-species biofilms were significantly thicker and tighter than the single-species biofilms.

  11. "The Lady in Pink: Dress and the Enigma of Gendered Space in Marcel Proust's Fiction"

    Directory of Open Access Journals (Sweden)

    Eva Maria Stadler

    2005-06-01

    Full Text Available A study of the role of clothing as central to issues of characterization, description and historical reference in Marcel Proust's A la recherche du temps perdu. Focus on Odette de Crécy, one of the central characters in the novel, a courtesan who becomes the wife of Charles Swann but who first captivates the narrator's imagination when, as a child, he briefly sees her as a "Lady in Pink." Odette's role as a fashionable woman, as one of the best-dressed women in Parisian society, gives unity to her character. The description of her clothing, however, not only provides the occasion for an accurate recreation of contemporary dress codes. The links between clothing and a woman's body are explicitly explored in creating the character of Odette. Her femininity is defined specifically in terms of surfaces and objects and her personality seems to assume its reality from costume. Dress not only encloses her lovely appearance; it gives substance to her person and order to her life. Through the agency of dress this ordinary, even vulgar woman rises above her condition and enters a world of passion and poetry.

  12. An overlooked pink species of land iguana in the Galápagos

    Science.gov (United States)

    Gentile, Gabriele; Fabiani, Anna; Marquez, Cruz; Snell, Howard L.; Snell, Heidi M.; Tapia, Washington; Sbordoni, Valerio

    2009-01-01

    Despite the attention given to them, the Galápagos have not yet finished offering evolutionary novelties. When Darwin visited the Galápagos, he observed both marine (Amblyrhynchus) and land (Conolophus) iguanas but did not encounter a rare pink black-striped land iguana (herein referred to as “rosada,” meaning “pink” in Spanish), which, surprisingly, remained unseen until 1986. Here, we show that substantial genetic isolation exists between the rosada and syntopic yellow forms and that the rosada is basal to extant taxonomically recognized Galápagos land iguanas. The rosada, whose present distribution is a conundrum, is a relict lineage whose origin dates back to a period when at least some of the present-day islands had not yet formed. So far, this species is the only evidence of ancient diversification along the Galápagos land iguana lineage and documents one of the oldest events of divergence ever recorded in the Galápagos. Conservation efforts are needed to prevent this form, identified by us as a good species, from extinction. PMID:19124773

  13. Viable mass production method for cotton pink bollworm, Pectinophora gossypiella (Saunders

    Directory of Open Access Journals (Sweden)

    B. Dhara Jothi

    2016-01-01

    Full Text Available Cotton seed based artificial diet has been standardized for continuous rearing of pink bollworm Pectinophora gossypiella (Saunders at the Central Institute for Cotton Research, Regional Station, Coimbatore. The ingredients of the diet are easily available and are cost effective. Basic ingredients of the diet are cotton seed flour (processed and chick pea flour, Carbohydrate, Protein, Fat sources, multi vitamin, antimicrobial agents and agar as thickening agent are used as other ingredients. Micro centrifuge tubes with lid were used as rearing containers. Individual neonate larvae were released on each piece of the diet inside the micro centrifuge tube and the lids were closed. This prevented larval escape, retaining them inside the tubes and also prevented diet dehydration. The recovery of insect reared on diet was recorded as 95.56%. Egg hatchability and adult emergence were 100% while pupal malformation was nil. Eggs, larval and pupal periods were recorded as 4.8 ± 0.632, 25.10 ± 0.994 and 7.9 ± 0.88 days, respectively. Larval and pupal weights were recorded as 21.40 mg ± 3.63, 18.00 mg ± 2.73, respectively.

  14. [Reproduction of the pink shrimp Farfantepenaeus notialis (Decapoda: Penaeidae) in the Colombian Caribbean].

    Science.gov (United States)

    Paramo, Jorge; Pérez, Daniel; Wolff, Matthias

    2014-06-01

    The shallow water pink shrimp (Farfantepenaeus notialis) is among the socioeconomically most important resources of the Caribbean. The lack of biological and fishery information is of great concern for the fisheries management authorities. The presented study therefore aimed at the investigation of the reproductive cycle, the size composition and the size at first maturity of this species as a basis for the ordination and management of this resource. The study was conducted from June 2012 to May 2013 off the coast of the Caribbean Sea of Colombia. A total of 5 356 individuals were collected, identified, classified and preserved for their subsequent analysis in the laboratory. Size, weight, sex and gonad stage were recorded for each specimen. Significant differences were found in sex ratio in all months sampled with a clear predominance of females. Mature females were found year-around, but two reproductive peaks were identified during the periods October-December and April-June. The mean catch total length size (MCS) for females and males was 148.00mm and 122.54mm, respectively. The mean size at maturity (LT50%) was 129.34mm for females and 97.77mm for males. MCS was always above LT50% for both sexes. Considering the large reduction in fishing effort in the Colombian Caribbean Sea over the last years, we could expect that the shrimp population is in a rebuilding process or perhaps it may be already restored.

  15. The Tarawera eruption, Lake Rotomahana, and the origin of the Pink and White Terraces

    Science.gov (United States)

    Keam, Ronald F.

    2016-03-01

    This chapter introduces the historical and geographical background for the scientific studies at Tarawera and Lake Rotomahana in the Taupo Volcanic Zone of New Zealand as detailed in this Special Issue of the Journal of Volcanology and Geothermal Research. It also presents the results of some original investigations. These are based partly on the large body of historical information that exists about the 1886 Tarawera eruption and the geothermal system at Rotomahana, and partly on the results of dedicated geological studies by other researchers within the Okataina Volcanic Centre where the historical events took place. Specifically, the new material here presented includes a detailed analysis of a previously almost neglected narrative by the only observer to witness the 1886 eruption from the southeast of the erupting craters and leave an account of his observations. The importance of a co-operative interplay between pre-existing tectonic deformation and its responses to strong seismic activity induced by magmatic intrusion is emphasised as being a major determinant in the course of the eruption, and as the main trigger of the eruption explosions that were audible throughout half of the land area of New Zealand. The chapter then concentrates on showing how the recent geological studies, in conjunction with ideas on the architecture of geysers, permit an explanation to be given as to how the unique Pink and White Terraces came to be formed.

  16. Methylobacterium gossipiicola sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere.

    Science.gov (United States)

    Madhaiyan, Munusamy; Poonguzhali, Selvaraj; Senthilkumar, Murugaiyan; Lee, Jung-Sook; Lee, Keun-Chul

    2012-01-01

    A pink, aerobic, facultatively methylotrophic, motile, Gram-negative rod, designated Gh-105(T), was isolated from the phyllosphere of cotton from Coimbatore (Tamilnadu, India). 16S rRNA gene sequence analysis showed clearly that the isolate belonged to the Methylobacterium cluster. Strain Gh-105(T) was most closely related to Methylobacterium adhaesivum AR27(T) (99% 16S rRNA gene sequence similarity) and Methylobacterium iners 5317S-33(T) (97.5%). The isolate grew with C(1) compounds such as methanol and dichloromethane, but not with formaldehyde, formate, methylamine, trimethylamine or methane, as sole carbon sources and carried mxaF, which encodes methanol dehydrogenase and supports methylotrophic metabolism. The major fatty acid was C(18:1)ω7c and the G+C content of the genomic DNA was 64.2 mol%. Physiological and biochemical data and DNA-DNA relatedness with M. adhaesivum KACC 12195(T) and M. iners KACC 11765(T) revealed clear phenotypic and genotypic differences. For this reason, we propose that strain Gh-105(T) (=CCM 7572(T) =NRRL B-51692(T)) represents the type strain of a novel species, with the name Methylobacterium gossipiicola sp. nov.

  17. A pink-pigmented, oxidative, nonmotile bacterium as a cause of opportunistic infections.

    Science.gov (United States)

    Korvick, J A; Rihs, J D; Gilardi, G L; Yu, V L

    1989-06-01

    We describe two cases of bacteremia due to a pink-pigmented, oxidative, nonmotile, gram-negative, rod-shaped organism. One case occurred in a febrile neutropenic patient and another in a chronically debilitated patient with pancreatic abscess. The first patient was cured with gentamicin and ticarcillin, but the second patient died while receiving cefamandole therapy. The organisms described here are similar to Methylobacterium mesophilicum (Pseudomonas mesophilica) and the "unnamed taxon" organisms. A major difference from M mesophilicum is the lack of methanol utilization. Further distinctions between our isolates and M mesophilicum are the lack of flagella in our organisms, growth at 42 degrees C, growth on MacConkey's agar, lack of acetamide assimilation, and citrate utilization. The lack of flagella is the principle difference between our isolates and those in the unnamed taxon. Both of the isolates were resistant to the cephalosporins, but susceptible to the aminoglycosides, ticarcillin-clavulanic acid, sulfamethoxazole and trimethoprim, and imipenem. With the growing population of immunocompromised and chronically ill patients, these organisms may emerge as important pathogens.

  18. Cesiribacter roseus sp. nov., a pink-pigmented bacterium isolated from desert sand.

    Science.gov (United States)

    Liu, Ming; Qi, Huan; Luo, Xuesong; Dai, Jun; Peng, Fang; Fang, Chengxiang

    2012-01-01

    A pink-pigmented, Gram-negative, rod-shaped, motile, strictly aerobic bacterium, designated strain 311(T), was isolated from desert sand in Xinjiang, China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 311(T) was related closely to Cesiribacter andamanensis AMV16(T) (94.6% similarity). The DNA G+C content of strain 311(T) was 47.1 mol% and the major respiratory quinone was menaquinone 7 (MK-7). The main cellular fatty acids were C(16:1)ω5c (29.9%), iso-C(15:0) (21.9%), iso-C(17:0) 3-OH (13.3%) and summed feature 4 (iso-C(17:1) I and/or anteiso-C(17:1) B; 13.0%). Based on phenotypic and chemotaxonomic data and phylogenetic analysis, strain 311(T) is considered to represent a novel species of the genus Cesiribacter, for which the name Cesiribacter roseus sp. nov. is proposed. The type strain is 311(T) (=CCTCC AB 207142(T) =KACC 15456(T)).

  19. Dominant lethal mutations in insects with holokinetic chromosomes: irradiation of pink bollworm sperm

    International Nuclear Information System (INIS)

    Berg, G.J.; LaChance, L.E.

    1976-01-01

    Adult males of the pink bollworm, Pectinophora gosypiella (Saunders), were irradiated with 19 and 30 krad of gamma radiation and mated with virgin, untreated females. Males treated with 19 or 30 krad of gamma radiation, at 2 to 24-h or 48 to 72-h postemergence, respectively, did not show reduced mating frequency compared with the untreated male controls. However, transfer of eupyrene sperm was reduced by treating 2 to 24-h postemergent males with 30 krad. Irradiation with 19 or 30 krad did not cause complete male sterility; 12.7 and 16.8 percent, respectively, of the fertilized eggs hatched. Eggs fertilized with irradiated sperm were examined cytologically and showed a retardation of embryonic development up to the blastoderm stage. From the blastoderm stage onward, development was parallel to those eggs which were fertilized by unirradiated sperm. Of the embryos in the groups treated with 30 and 19 krad, 51.3 to 66.6 percent, respectively, developed into fully differentiated, normal-appearing, prehatch embryos. The radiation-induced dominant lethal mutations were, generally, expressed very late in embryonic development

  20. Complementation analyses for 45 mutations encompassing the pink-eyed dilution (p) locus of the mouse

    Energy Technology Data Exchange (ETDEWEB)

    Russell, L.B.; Montgomery, C.S.; Cacheiro, N.L.A.; Johnson, D.K. [Oak Ridge National Lab., TN (United States)

    1995-12-01

    The homozygous and heterozygous phenotypes are described and characterized for 45 new pink-eyed dilution (p) locus mutations, most of them radiation-induced, that affect survival at various stages of mouse development. Cytogenetically detectable aberrations were found in three of the new p mutations (large deletion, inversion, translocation), with band 7C involved in each case. The complementation map developed from the study of 810 types of compound heterozygotes identifies five functional units: jls and jlm (two distinct juvenile-fitness functions, the latter associated with neuromuscular defects), pl-1 and pl-2 (associated with early-postimplantation and preimplantation death, respectively), and nl [neonatal lethality associated with cleft palate (the frequency of rare {open_quotes}escapers{close_quotes} from this defect varied with the genotype)]. Orientation of these units relative to genetic markers is as follows: centromere, Gas-2, pl-1, jls, jlm p, nl (equatable to cp1= Gabrb3); pl-2 probably resides in the c-deletion complex. pl-1 does not mask preimplantation lethals between Gas2 and p; and no genes affecting survival are located between p and cp1. The alleles specifying mottling or darker pigment (generically, p{sup m} and p{sup x}, respectively) probably do not represent deletions of p-coding sequences but could be small rearrangements involving proximal regulatory elements. 43 refs., 5 figs., 7 tabs.

  1. Prevention of pink-pigmented methylotrophic bacteria (Methylohacterium mesophilicum) contamination of plant tissue cultures.

    Science.gov (United States)

    Chanprame, S; Todd, J J; Widholm, J M

    1996-12-01

    Pink-pigmented facultative methylotrophic bacteria (PPFMs) have been found on the surfaces of leaves of most plants tested. We found PPFMs on the leaf surfaces of all 40 plants (38 species) tested and on soybean pods by pressing onto AMS medium with methanol as the sole carbon source. The abundance ranged from 0.5 colony forming unit (cfu) /cm(2) to 69.4 cfu/cm(2) on the leaf surfaces. PPFMs were found in homogenized leaf tissues of only 4 of the species after surface disinfestation with 1.05% sodium hypochlorite and were rarely found in cultures initiated from surface disinfested Datura innoxia leaves or inside surface disinfested soybean pods. Of 20 antibiotics tested for PPFM growth inhibition, rifampicin was the most effective and of seven others which also inhibited PPFM growth, cefotaxime should be the most useful due to the expected low plant cell toxicity. These antibiotics could be used in concert with common surface sterilization procedures to prevent the introduction or to eliminate PPFM bacteria in tissue cultures. Thus, while PPFMs are present on the surfaces of most plant tissues, surface disinfestation alone can effectively remove them so that uncontaminated tissue cultures can be initiated in most cases.

  2. Biotechnological and agronomic potential of endophytic pink-pigmented methylotrophic Methylobacterium spp.

    Science.gov (United States)

    Dourado, Manuella Nóbrega; Camargo Neves, Aline Aparecida; Santos, Daiene Souza; Araújo, Welington Luiz

    2015-01-01

    The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM) bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one carbon (C1), such as methanol and methylamine. Due to their high phenotypic plasticity, these bacteria are able to colonize different habitats, such as soil, water, and sediment, and different host plants as both endophytes and epiphytes. In plant colonization, the frequency and distribution may be influenced by plant genotype or by interactions with other associated microorganisms, which may result in increasing plant fitness. In this review, different aspects of interactions with the host plant are discussed, including their capacity to fix nitrogen, nodule the host plant, produce cytokinins, auxin and enzymes involved in the induction of systemic resistance, such as pectinase and cellulase, and therefore plant growth promotion. In addition, bacteria belonging to this group can be used to reduce environmental contamination because they are able to degrade toxic compounds, tolerate high heavy metal concentrations, and increase plant tolerance to these compounds. Moreover, genome sequencing and omics approaches have revealed genes related to plant-bacteria interactions that may be important for developing strains able to promote plant growth and protection against phytopathogens.

  3. Evaluation of pink-pigmented facultative methylotrophic bacteria for phosphate solubilization.

    Science.gov (United States)

    Jayashree, Shanmugam; Vadivukkarasi, Ponnusamy; Anand, Kirupanithi; Kato, Yuko; Seshadri, Sundaram

    2011-08-01

    Thirteen pink-pigmented facultative methylotrophic (PPFM) strains isolated from Adyar and Cooum rivers in Chennai and forest soil samples in Tamil Nadu, India, along with Methylobacterium extorquens, M. organophilum, M. gregans, and M. komagatae were screened for phosphate solubilization in plates. P-solubilization index of the PPFMs grown on NBRIP-BPB plates for 7 days ranged from 1.1 to 2.7. The growth of PPFMs in tricalcium phosphate amended media was found directly proportional to the glucose concentration. Higher phosphate solubilization was observed in four strains MSF 32 (415 mg l(-l)), MDW 80 (301 mg l(-l)), M. komagatae (279 mg l(-l)), and MSF 34 (202 mg l(-l)), after 7 days of incubation. A drop in the media pH from 6.6 to 3.4 was associated with an increase in titratable acidity. Acid phosphatase activity was more pronounced in the culture filtrate than alkaline phosphatase activity. Adherence of phosphate to densely grown bacterial surface was observed under scanning electron microscope after 7-day-old cultures. Biochemical characterization and screening for methanol dehydrogenase gene (mxaF) confirmed the strains as methylotrophs. The mxaF gene sequence from MSF 32 clustered towards M. lusitanum sp. with 99% similarity. This study forms the first detailed report on phosphate solubilization by the PPFMs.

  4. Methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice.

    Science.gov (United States)

    Madhaiyan, Munusamy; Poonguzhali, Selvaraj; Kwon, Soon-Wo; Sa, Tong-Min

    2009-01-01

    A pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, CBMB27T, isolated from leaf tissues of rice (Oryza sativa L. 'Dong-Jin'), was analysed using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium oryzae, Methylobacterium fujisawaense and Methylobacterium mesophilicum; strain CBMB27T showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strains of these three species. DNA-DNA hybridization experiments revealed low levels (<38 %) of DNA-DNA relatedness between strain CBMB27T and its closest relatives. The sequence of the 1-aminocyclopropane-1-carboxylate deaminase gene (acdS) in strain CBMB27T differed from those of close relatives. The major fatty acid of the isolate was C(18 : 1)omega7c and the G+C content of the genomic DNA was 66.8 mol%. Based on the results of 16S rRNA gene sequence analysis, DNA-DNA hybridization, and physiological and biochemical characterization, which enabled the isolate to be differentiated from all recognized species of the genus Methylobacterium, it was concluded that strain CBMB27T represents a novel species in the genus Methylobacterium for which the name Methylobacterium phyllosphaerae sp. nov. is proposed (type strain CBMB27T =LMG 24361T =KACC 11716T =DSM 19779T).

  5. Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented Methylotrophic Methylobacterium spp.

    Directory of Open Access Journals (Sweden)

    Manuella Nóbrega Dourado

    2015-01-01

    Full Text Available The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one carbon (C1, such as methanol and methylamine. Due to their high phenotypic plasticity, these bacteria are able to colonize different habitats, such as soil, water, and sediment, and different host plants as both endophytes and epiphytes. In plant colonization, the frequency and distribution may be influenced by plant genotype or by interactions with other associated microorganisms, which may result in increasing plant fitness. In this review, different aspects of interactions with the host plant are discussed, including their capacity to fix nitrogen, nodule the host plant, produce cytokinins, auxin and enzymes involved in the induction of systemic resistance, such as pectinase and cellulase, and therefore plant growth promotion. In addition, bacteria belonging to this group can be used to reduce environmental contamination because they are able to degrade toxic compounds, tolerate high heavy metal concentrations, and increase plant tolerance to these compounds. Moreover, genome sequencing and omics approaches have revealed genes related to plant-bacteria interactions that may be important for developing strains able to promote plant growth and protection against phytopathogens.

  6. Value of information in natural resource management: technical developments and application to pink-footed geese

    Science.gov (United States)

    Williams, Byron K.; Johnson, Fred A.

    2015-01-01

    The “value of information” (VOI) is a generic term for the increase in value resulting from better information to guide management, or alternatively, the value foregone under uncertainty about the impacts of management (Yokota and Thompson, Medical Decision Making 2004;24: 287). The value of information can be characterized in terms of several metrics, including the expected value of perfect information and the expected value of partial information. We extend the technical framework for the value of information by further developing the relationship between value metrics for partial and perfect information and describing patterns of their performance. We use two different expressions for the expected value of partial information to highlight its relationship to the expected value of perfect information. We also develop the expected value of partial information for hierarchical uncertainties. We highlight patterns in the value of information for the Svalbard population of the pink-footed goose (Anser brachyrhynchus), a population that is subject to uncertainty in both reproduction and survival functions. The framework for valuing information is seen as having widespread potential in resource decision making, and serves as a motivation for resource monitoring, assessment, and collaboration.

  7. A study on the isolation of protoplasts from mesophyll cells of Dendrobium Queen Pink

    International Nuclear Information System (INIS)

    Aqeel, R.; Zehra, M.; Kazmi, S. K.; Khan, S.

    2016-01-01

    Protoplasts were successfully isolated from one month old In vitro grown plantlets of Dendrobium cultivar Queen pink. The enzyme solution used was composed of 1 percent Cellulase Onozuka R-10, 0.5 percent Macerozyme R-10, 0.1 percent Pectinase, 0.3 M mannitol, 10 mM CaCl/sub 2/.2H/sub 2/O and 10 mM 2 (N-morpholino)-ethanesulfonic acid (MES) at pH 5.8. Protoplast highest yield with 15.7x104 protoplasts per 1.5 gm freshly chopped leaves were obtained when digested in enzyme solution for 4 hrs on a rotary shaker with an agitation speed of 45 rpm in dark conditions. Protoplasts were filtered with 45 micro m nylon sieve and washed with 0.3 M mannitol solution supplemented with 10 mM CaCl/sub 2/.2H/sub 2/O and 10 mM MES, and purified with 0.3 M sucrose solution gradient. Purification of protoplasts on a sucrose mannitol gradient yielded clean protoplasts that were free from debris. (author)

  8. Curcumin Rescues a PINK1 Knock Down SH-SY5Y Cellular Model of Parkinson's Disease from Mitochondrial Dysfunction and Cell Death.

    Science.gov (United States)

    van der Merwe, Celia; van Dyk, Hayley Christy; Engelbrecht, Lize; van der Westhuizen, Francois Hendrikus; Kinnear, Craig; Loos, Ben; Bardien, Soraya

    2017-05-01

    Parkinson's disease (PD) is a neurodegenerative disorder characterised by the loss of dopaminergic neurons in the substantia nigra. Mutations in the PINK1 gene result in an autosomal recessive form of early-onset PD. PINK1 plays a vital role in mitochondrial quality control via the removal of dysfunctional mitochondria. The aim of the present study was to create a cellular model of PD using siRNA-mediated knock down of PINK1 in SH-SY5Y neuroblastoma cells The possible protective effects of curcumin, known for its many beneficial properties including antioxidant and anti-inflammatory effects, was tested on this model in the presence and absence of paraquat, an additional stressor. PINK1 siRNA and control cells were separated into four treatment groups: (i) untreated, (ii) treated with paraquat, (iii) pre-treated with curcumin then treated with paraquat, or (iv) treated with curcumin. Various parameters of cellular and mitochondrial function were then measured. The PINK1 siRNA cells exhibited significantly decreased cell viability, mitochondrial membrane potential (MMP), mitochondrial respiration and ATP production, and increased apoptosis. Paraquat-treated cells exhibited decreased cell viability, increased apoptosis, a more fragmented mitochondrial network and decreased MMP. Curcumin pre-treatment followed by paraquat exposure rescued cell viability and increased MMP and mitochondrial respiration in control cells, and significantly decreased apoptosis and increased MMP and maximal respiration in PINK1 siRNA cells. These results highlight a protective effect of curcumin against mitochondrial dysfunction and apoptosis in PINK1-deficient and paraquat-exposed cells. More studies are warranted to further elucidate the potential neuroprotective properties of curcumin.

  9. Binding and Oligomerization of Modified and Native Bt Toxins in Resistant and Susceptible Pink Bollworm.

    Directory of Open Access Journals (Sweden)

    Josue Ocelotl

    Full Text Available Insecticidal proteins from Bacillus thuringiensis (Bt are used extensively in sprays and transgenic crops for pest control, but their efficacy is reduced when pests evolve resistance. Better understanding of the mode of action of Bt toxins and the mechanisms of insect resistance is needed to enhance the durability of these important alternatives to conventional insecticides. Mode of action models agree that binding of Bt toxins to midgut proteins such as cadherin is essential for toxicity, but some details remain unresolved, such as the role of toxin oligomers. In this study, we evaluated how Bt toxin Cry1Ac and its genetically engineered counterpart Cry1AcMod interact with brush border membrane vesicles (BBMV from resistant and susceptible larvae of Pectinophora gossypiella (pink bollworm, a global pest of cotton. Compared with Cry1Ac, Cry1AcMod lacks 56 amino acids at the amino-terminus including helix α-1; previous work showed that Cry1AcMod formed oligomers in vitro without cadherin and killed P. gossypiella larvae harboring cadherin mutations linked with >1000-fold resistance to Cry1Ac. Here we found that resistance to Cry1Ac was associated with reduced oligomer formation and insertion. In contrast, Cry1AcMod formed oligomers in BBMV from resistant larvae. These results confirm the role of cadherin in oligomerization of Cry1Ac in susceptible larvae and imply that forming oligomers without cadherin promotes toxicity of Cry1AcMod against resistant P. gossypiella larvae that have cadherin mutations.

  10. Methylobacterium pseudosasae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the bamboo phyllosphere.

    Science.gov (United States)

    Madhaiyan, Munusamy; Poonguzhali, Selvaraj

    2014-02-01

    A pink-pigmented, Gram negative, aerobic, facultatively methylotrophic bacterium, strain BL44(T), was isolated from bamboo leaves and identified as a member of the genus Methylobacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed similarity values of 98.7-97.0 % with closely related type strains and showed highest similarity to Methylobacterium zatmanii DSM 5688(T) (98.7 %) and Methylobacterium thiocyanatum DSM 11490(T) (98.7 %). Methylotrophic metabolism in this strain was confirmed by PCR amplification and sequencing of the mxaF gene coding for the α-subunit of methanol dehydrogenase. Strain BL44(T) produced three known quorum sensing signal molecules with similar retention time to C8, C10 and C12-HSLs when characterized by GC-MS. The fatty acid profiles contained major amounts of C18:1 ω7c, iso-3OH C17:0 and summed feature 3 (C16:1 ω7c and/or iso-C15:0 2-OH), which supported the grouping of the isolate in the genus Methylobacterium. The DNA G+C content was 66.9 mol%. DNA relatedness of the strain BL44(T) to its most closely related strains ranged from 12-43.3 %. On the basis of the phenotypic, phylogenetic and DNA-DNA hybridization data, strain BL44(T) is assigned to a novel species of the genus Methylobacterium for which the name Methylobacterium pseudosasae sp. nov. is proposed (type strain BL44(T) = NBRC 105205(T) = ICMP 17622(T)).

  11. Go Pink! The Effect of Secondary Quanta on Detective Quantum Efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Watson, Scott [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-09-05

    Photons are never directly observable. Consequently, we often use photoelectric detectors (eg CCDs) to record associated photoelectrons statistically. Nonetheless, it is an implicit goal of radiographic detector designers to achieve the maximum possible detector efficiency1. In part the desire for ever higher efficiency has been due to the fact that detectors are far less expensive than associated accelerator facilities (e.g. DARHT and PHERMEX2). In addition, higher efficiency detectors often have better spatial resolution. Consequently, the optimization of the detector, not the accelerator, is the system component with the highest leverage per dollar. In recent years, imaging scientists have adopted the so-called Detective Quantum Efficiency, or DQE as a summary measure of detector performance. Unfortunately, owing to the complex nature of the trade-space associated with detector components, and the natural desire for simplicity and low(er) cost, there has been a recent trend in Los Alamos to focus only on the zerofrequency efficiency, or DQE(0), when designing such systems. This narrow focus leads to system designs that neglect or even ignore the importance of high-spatial-frequency image components. In this paper we demonstrate the significant negative impact of these design choices on the Noise Power Spectrum1 (NPS) and recommend a more holistic approach to detector design. Here we present a statistical argument which indicates that a very large number (>20) of secondary quanta (typically visible light and/or recorded photo-electrons) are needed to take maximum advantage of the primary quanta (typically x-rays or protons) which are available to form an image. Since secondary particles come in bursts, they are not independent. In short, we want to maximize the pink nature of detector noise at DARHT.

  12. Rearing and gamma radiation effects on mature pupae of pink bollworm and their F1 progeny

    International Nuclear Information System (INIS)

    Qureshi, Z.A.; Ahmed, N.; Hussain, T.

    1993-01-01

    Pink bollworm larvae were successfully reared in captivity on a casein wheat germ diet. The substitution of casein with soyflour, corn-cob grit and wheat germ, and casein for peanut flour, resulted in delayed development, reduced pupal recovery and fecundity of the adult moths. This reduction was more drastic in corn-cob grit and peanut flour diets. The irradiation of mature pupae at 50-200 Gy resulted in decreased adult emergence with increased gamma radiation doses, and more deformed moths were recorded at a dose of 200 Gy. Adults following irradiation of mature pupae when crossed with untreated males or females or treated individuals crossed to treated exhibited reduced fecundity and fertility with the increasing doses. This reduction was more pronounced when treated males were crossed with treated females. Females were relatively more sensitive to gamma radiation, as a reduced number of eggs was obtained when treated females were crossed with untreated males. At 200 Gy, no F 1 progeny were obtained from any cross involving treated parents. The fecundity and fertility were reduced significantly when F 1 males or F 1 females from male parents irradiated as mature pupae were mated with untreated insects at both 100 and 150 Gy. However, inherited sterility was more pronounced when F 1 males were crossed with untreated females than when F 1 females were crossed with untreated males. Similarly reduced fecundity and fertility in F 1 progeny from female parents irradiated as mature pupae, both at 100 and 150 Gy, were also recorded in crosses as described for male F 1 progeny. The fecundity and fertility were the lowest in F 1 progeny of both male and female parents irradiated as mature pupae when compared with the F 1 progeny of male or female irradiated parents separately. (author). 28 refs, 7 tabs

  13. Effects of Short-Term Presalting and Salt Level on the Development of Pink Color in Cooked Chicken Breasts

    OpenAIRE

    Jeong, Jong Youn

    2017-01-01

    The objective of this study was to determine the effects of short-term presalting on pink color and pigment characteristics in ground chicken breasts after cooking. Four salt levels (0%, 1%, 2%, and 3%) were presalted and stored for 0 and 3 d prior to cooking. Cooking yield was increased as salt level was increased. However, no significant differences in pH values or oxidation reduction potential (ORP) of cooked chicken breasts were observed. Cooked products with more than 2% of salt level ha...

  14. The EDTA effect on phytoextraction of single and combined metals-contaminated soils using rainbow pink (Dianthus chinensis).

    Science.gov (United States)

    Lai, Hung-Yu; Chen, Zueng-Sang

    2005-08-01

    Rainbow pink (Dianthus chinensis), a potential phytoextraction plant, can accumulate high concentrations of Cd from metal-contaminated soils. The soils used in this study were artificially added with different metals including (1) CK: original soil, (2) Cd-treated soil: 10 mg Cd kg(-1), (3) Zn-treated soil: 100 mg Zn kg(-1), (4) Pb-treated soil: 1000 mg Pb kg(-1), (5) Cd-Zn-treated soil: 10 mg Cd kg(-1) and 100 mg Zn kg(-1), (6) Cd-Pb-treated soil: 10 mg Cd kg(-1) and 1000 mg Pb kg(-1), (7) Zn-Pb-treated soil: 100 mg Zn kg(-1) and 1000 mg Pb kg(-1), and (8) Cd-Zn-Pb-treated soil: 10 mg Cd kg(-1), 100 mg Zn kg(-1), and 1000 mg Pb kg(-1). Three concentrations of 2Na-EDTA solutions (0 (control), 2, and 5 mmol kg(-1) soil) were added to the different metals-treated soils to study the influence of applied EDTA on single and combined metals-contaminated soils phytoextraction using rainbow pink. The results showed that the Cd, Zn, Pb, Fe, or Mn concentrations in different metals-treated soil solutions significantly increased after applying 5 mmol EDTA kg(-1) (p<0.05). The metal concentrations in different metals-treated soils extracted by deionized water also significantly increased after applying 5 mmol EDTA kg(-1) (p<0.05). Because of the high extraction capacity of both 0.005 M DTPA (pH 5.3) and 0.05 M EDTA (pH 7.0), applying EDTA did not significantly increase the Cd, Zn, or Pb concentration in both extracts for most of the treatments. Applying EDTA solutions can significantly increase the Cd and Pb concentrations in the shoots of rainbow pink (p<0.05). However, this was not statistically significant for Zn because of the low Zn concentration added into the contaminated soils. The results from this study indicate that applying 5 mmol EDTA kg(-1) can significantly increase the Cd, Zn, or Pb concentrations both in the soil solution or extracted using deionized water in single or combined metals-contaminated soils, thus increasing the accumulated metals concentrations in

  15. Body Mass Dynamics of Pink-footed Geese (Anser brachyrhynchus) During Stopover on Autumn Migration in Norway

    DEFF Research Database (Denmark)

    Gundersen, Ove Martin; Clausen, Kevin; Madsen, Jesper

    2017-01-01

    Body mass accumulation is a widely used measure of waterfowl condition and predictor of fitness. So far, however, post-breeding changes in body mass affecting autumn and winter condition have been largely unexplored. Here, changes in body mass of Pink-footed Geese (Anser brachyrhynchus) were...... adult body mass did not vary between years. During the stopover, juveniles of both sexes increased their body mass substantially (11.4 ± 2.8 g/day), while adult birds showed sex-specific differences. Adult males took on an average of 6.1 ± 2.4 g/day, whereas adult females showed no increase during...

  16. Ameliorating mitochondrial dysfunction restores carbon ion-induced cognitive deficits via co-activation of NRF2 and PINK1 signaling pathway

    Directory of Open Access Journals (Sweden)

    Yang Liu

    2018-07-01

    Full Text Available Carbon ion therapy is a promising modality in radiotherapy to treat tumors, however, a potential risk of induction of late normal tissue damage should still be investigated and protected. The aim of the present study was to explore the long-term cognitive deficits provoked by a high-linear energy transfer (high-LET carbon ions in mice by targeting to hippocampus which plays a crucial role in memory and learning. Our data showed that, one month after 4 Gy carbon ion exposure, carbon ion irradiation conspicuously resulted in the impaired cognitive performance, neurodegeneration and neuronal cell death, as well as the reduced mitochondrial integrity, the disrupted activities of tricarboxylic acid cycle flux and electron transport chain, and the depressed antioxidant defense system, consequently leading to a decline of ATP production and persistent oxidative damage in the hippocampus region. Mechanistically, we demonstrated the disruptions of mitochondrial homeostasis and redox balance typically characterized by the disordered mitochondrial dynamics, mitophagy and glutathione redox couple, which is closely associated with the inhibitions of PINK1 and NRF2 signaling pathway as the key regulators of molecular responses in the context of neurotoxicity and neurodegenerative disorders. Most importantly, we found that administration with melatonin as a mitochondria-targeted antioxidant promoted the PINK1 accumulation on the mitochondrial membrane, and augmented the NRF2 accumulation and translocation. Moreover, melatonin pronouncedly enhanced the molecular interplay between NRF2 and PINK1. Furthermore, in the mouse hippocampal neuronal cells, overexpression of NRF2/PINK1 strikingly protected the hippocampal neurons from carbon ion-elicited toxic insults. Thus, these data suggest that alleviation of the sustained mitochondrial dysfunction and oxidative stress through co-modulation of NRF2 and PINK1 may be in charge of restoration of the cognitive

  17. Enhancement of high glucose-induced PINK1 expression by melatonin stimulates neuronal cell survival: Involvement of MT2 /Akt/NF-κB pathway.

    Science.gov (United States)

    Onphachanh, Xaykham; Lee, Hyun Jik; Lim, Jae Ryong; Jung, Young Hyun; Kim, Jun Sung; Chae, Chang Woo; Lee, Sei-Jung; Gabr, Amr Ahmed; Han, Ho Jae

    2017-09-01

    Hyperglycemia is a representative hallmark and risk factor for diabetes mellitus (DM) and is closely linked to DM-associated neuronal cell death. Previous investigators reported on a genome-wide association study and showed relationships between DM and melatonin receptor (MT), highlighting the role of MT signaling by assessing melatonin in DM. However, the role of MT signaling in DM pathogenesis is unclear. Therefore, we investigated the role of mitophagy regulators in high glucose-induced neuronal cell death and the effect of melatonin against high glucose-induced mitophagy regulators in neuronal cells. In our results, high glucose significantly increased PTEN-induced putative kinase 1 (PINK1) and LC-3B expressions; as well it decreased cytochrome c oxidase subunit 4 expression and Mitotracker™ fluorescence intensity. Silencing of PINK1 induced mitochondrial reactive oxygen species (ROS) accumulation and mitochondrial membrane potential impairment, increased expressions of cleaved caspases, and increased the number of annexin V-positive cells. In addition, high glucose-stimulated melatonin receptor 1B (MTNR1B) mRNA and PINK1 expressions were reversed by ROS scavenger N-acetyl cysteine pretreatment. Upregulation of PINK1 expression in neuronal cells is suppressed by pretreatment with MT 2 receptor-specific inhibitor 4-P-PDOT. We further showed melatonin stimulated Akt phosphorylation, which was followed by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) phosphorylation and nuclear translocation. Silencing of PINK1 expression abolished melatonin-regulated mitochondrial ROS production, cleaved caspase-3 and caspase-9 expressions, and the number of annexin V-positive cells. In conclusion, we have demonstrated the melatonin stimulates PINK1 expression via an MT 2 /Akt/NF-κB pathway, and such stimulation is important for the prevention of neuronal cell apoptosis under high glucose conditions. © 2017 The Authors. Journal of Pineal Research

  18. The impact of weather on women's tendency to wear red or pink when at high risk for conception.

    Directory of Open Access Journals (Sweden)

    Jessica L Tracy

    Full Text Available Women are particularly motivated to enhance their sexual attractiveness during their most fertile period, and men perceive shades of red, when associated with women, as sexually attractive. Building on this research, we recently found that women are more likely to wear reddish clothing when at peak fertility (Beall & Tracy, 2013, presumably as a way of increasing their attractiveness. Here, we first report results from a methodological replication, conducted during warmer weather, which produced a null effect. Investigating this discrepancy, we considered the impact of a potentially relevant contextual difference between previous research and the replication: current weather. If the red-dress effect is driven by a desire to increase one's sexual appeal, then it should emerge most reliably when peak-fertility women have few alternative options for accomplishing this goal (e.g., wearing minimal clothing. Results from re-analyses of our previously collected data and a new experiment support this account, by demonstrating that the link between fertility and red/pink dress emerges robustly in cold, but not warm, weather. Together, these findings suggest that the previously documented red-dress effect is moderated by current climate concerns, and provide further evidence that under certain circumstances red/pink dress is reliably associated with female fertility.

  19. Observation of intermediate bands in Eu3+ doped YPO4 host: Li+ ion effect and blue to pink light emitter

    Directory of Open Access Journals (Sweden)

    Abdul Kareem Parchur

    2012-09-01

    Full Text Available This article explores the tuning of blue to pink colour generation from Li+ ion co-doped YPO4:5Eu nanoparticles prepared by polyol method at ∼100-120 °C with ethylene glycol (EG as a capping agent. Interaction of EG molecules capped on the surface of the nanoparticles and/or created oxygen vacancies induces formation of intermediate/mid gap bands in the host structure, which is supported by UV-Visible absorption data. Strong blue and pink colors can be observed in the cases of as-prepared and 500 °C annealed samples, respectively. Co-doping of Li+ enhances the emission intensities of intermediate band as well as Eu3+. On annealing as-prepared sample to 500 °C, the intermediate band emission intensity decreases, whereas Eu3+ emission intensity increases suggesting increase of extent of energy transfer from the intermediate band to Eu3+ on annealing. Emission intensity ratio of electric to magnetic dipole transitions of Eu3+ can be varied by changing excitation wavelength. The X-ray photoelectron spectroscopy (XPS study of as-prepared samples confirms the presence of oxygen vacancies and Eu3+ but absence of Eu2+. Dispersed particles in ethanol and polymer film show the strong blue color, suggesting that these materials will be useful as probes in life science and also in light emitting device applications.

  20. Parkin and PINK1 Patient iPSC-Derived Midbrain Dopamine Neurons Exhibit Mitochondrial Dysfunction and α-Synuclein Accumulation

    Directory of Open Access Journals (Sweden)

    Sun Young Chung

    2016-10-01

    Full Text Available Parkinson's disease (PD is characterized by the selective loss of dopamine neurons in the substantia nigra; however, the mechanism of neurodegeneration in PD remains unclear. A subset of familial PD is linked to mutations in PARK2 and PINK1, which lead to dysfunctional mitochondria-related proteins Parkin and PINK1, suggesting that pathways implicated in these monogenic forms could play a more general role in PD. We demonstrate that the identification of disease-related phenotypes in PD-patient-specific induced pluripotent stem cell (iPSC-derived midbrain dopamine (mDA neurons depends on the type of differentiation protocol utilized. In a floor-plate-based but not a neural-rosette-based directed differentiation strategy, iPSC-derived mDA neurons recapitulate PD phenotypes, including pathogenic protein accumulation, cell-type-specific vulnerability, mitochondrial dysfunction, and abnormal neurotransmitter homeostasis. We propose that these form a pathogenic loop that contributes to disease. Our study illustrates the promise of iPSC technology for examining PD pathogenesis and identifying therapeutic targets.

  1. Parkin and PINK1 Patient iPSC-Derived Midbrain Dopamine Neurons Exhibit Mitochondrial Dysfunction and α-Synuclein Accumulation.

    Science.gov (United States)

    Chung, Sun Young; Kishinevsky, Sarah; Mazzulli, Joseph R; Graziotto, John; Mrejeru, Ana; Mosharov, Eugene V; Puspita, Lesly; Valiulahi, Parvin; Sulzer, David; Milner, Teresa A; Taldone, Tony; Krainc, Dimitri; Studer, Lorenz; Shim, Jae-Won

    2016-10-11

    Parkinson's disease (PD) is characterized by the selective loss of dopamine neurons in the substantia nigra; however, the mechanism of neurodegeneration in PD remains unclear. A subset of familial PD is linked to mutations in PARK2 and PINK1, which lead to dysfunctional mitochondria-related proteins Parkin and PINK1, suggesting that pathways implicated in these monogenic forms could play a more general role in PD. We demonstrate that the identification of disease-related phenotypes in PD-patient-specific induced pluripotent stem cell (iPSC)-derived midbrain dopamine (mDA) neurons depends on the type of differentiation protocol utilized. In a floor-plate-based but not a neural-rosette-based directed differentiation strategy, iPSC-derived mDA neurons recapitulate PD phenotypes, including pathogenic protein accumulation, cell-type-specific vulnerability, mitochondrial dysfunction, and abnormal neurotransmitter homeostasis. We propose that these form a pathogenic loop that contributes to disease. Our study illustrates the promise of iPSC technology for examining PD pathogenesis and identifying therapeutic targets. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  2. The Salmon Smai Family of Short Interspersed Repetitive Elements (Sines): Interspecific and Intraspecific Variation of the Insertion of Sines in the Genomes of Chum and Pink Salmon

    OpenAIRE

    Takasaki, N.; Yamaki, T.; Hamada, M.; Park, L.; Okada, N.

    1997-01-01

    The genomes of chum salmon and pink salmon contain a family of short interspersed repetitive elements (SINEs), designated the salmon SmaI family. It is restricted to these two species, a distribution that suggests that this SINE family might have been generated in their common ancestor. When insertions of the SmaI SINEs at 10 orthologous loci of these species were analyzed, however, it was found that there were no shared insertion sites between chum and pink salmon. Furthermore, at six loci w...

  3. Effects of seasonal variations on antioxidant activity of pink guava fruits

    Science.gov (United States)

    Ahmad, Haniza; Abdullah, Aminah

    2014-09-01

    This study aimed to evaluate the effects of seasonal variations during rainy and hot season on antioxidant activity of pink guava fruits in approximately one year duration specifically on November 2012, December 2012, January 2013, March 2013, April 2013, May 2013, July 2013, August 2013 and November 2013. Fruit samples (Sungkai and Semenyih variants) were collected from Sime Darby Beverages plantation located in Sitiawan. The fruits were samples for 9 times from Nov 2012 to Nov 2013 except Feb 2013, Jun 2013, Sept 2013 and Oct 2013. Fruits were peeled, seeded and blended into uniform puree. Samples were then extracted for its antioxidant activity determination using 50% acetone. Antioxidant activity was evaluated using total phenolic compounds (TPC) assay, ferric-reducing antioxidant power assay (FRAP) and 1,1-diphenyl1-2-picrylhydrazyl free radical-scavenging capacity (DPPH). Analysis was conducted using 96-well microplate spectrophotometer UV. The highest TPC result was Semenyih var recorded 2192.80 mg GAE/100g FW whilst Sungkai var 1595.98 mg GAE/100g FW both on July 2013 with rainfall was at the least (45mm) and the lowest for Sungkai var was 792.75 mg GAE/100g FW and 1032.41 mg GAE/100g FW for Semenyih var, both on Nov 2012 with 185mm rainfall. There were significant negative correlation between TPC and rainfall (mm) for both Semenyih var (r = - 0.699, p<0.005, r2 = 0.489) and Sungkai var (r = -0.72, p<0.05, r2 = 0.531). The highest FRAP result (mg TE/100g FW) was 1677.74 for Semenyih var (Aug 2013, rainfall = 160.5mm) and the highest FRAP for Sungkai var was 1104.60 (Jul 2013, rainfall = 45.0mm) whereas the lowest for Semenyih and Sungkai var was 1090.22 (Mar 2013, rainfall = 97.5mm) and 767.88 (Nov 2012, rainfall = 185.50) respectively. There was weak negative correlation between FRAP and rainfall(mm) for both Sungkai var (r = - 0.324, p<0.05, r2 = 0.105) and Semenyih var (r = - 0.362, p<0.05, r2 = 0.132). The highest DPPH for Semenyih var was 88.40% (Aug

  4. Kinetics of phase transformation and optical property of pink coral zirconia powders

    International Nuclear Information System (INIS)

    Chu, Hsueh-Liang; Wang, Cheng-Li; Hwang, Weng-Sing; Lee, Kuen-Chan; Zhou, Xuedong; Wang, Moo-Chin

    2014-01-01

    Highlights: • The single phase of tetragonal ZrO 2 formed when calcined at 1223 K for 1 h. • The tetragonal ZrO 2 fully converted to ZrSiO 4 when calcined at 1323–1473 K for 1 h. • The activation energy of t-ZrO 2 formed is 399.9 kJ/mol when 5 mol% Fe 2 O 3 added. • The activation energy of the Fe/ZrSiO 4 formed is 257.7 kJ/mol when 5 mol% Fe 2 O 3 added. • The growth morphology parameter and crystallization index are about 2.0 and 1.0. - Abstract: The kinetics of phase transformation and optical property of pink coral zircon powders have been studied. The ZrO 2 –SiO 2 –Fe 2 O 3 precursor powders were synthesized using Zr(NO 3 ) 4 ⋅4H 2 O, Si(C 2 H 5 O) 4 and Fe(NO 3 ) 3 ⋅9H 2 O as initial materials via the hot–wet routes. The kinetics of phase transformation of the ZrO 2 –SiO 2 –Fe 2 O 3 precursor powders was characterized by thermo-gravimetric (TG)/differential scanning calorimeter (DSC), X-ray diffraction (XRD), transmission electron microscopy (TEM), nano-beam electron diffraction (NBED), and spectrophotometry. The crystallization temperatures of tetragonal zirconia (t-ZrO 2 ) and zircon (ZrSiO 4 ) of ZrO 2 –SiO 2 precursor powders with 1 mol% Fe 2 O 3 were estimated to be approximately 1204 K and 1496 K, respectively, based on a DSC analysis conducted at a heating rate of 20 K/min. The activation energies of t-ZrO 2 formulation are 428.2, 403.2, and 399.9 kJ/mol, respectively, for ZrO 2 –SiO 2 precursor powders containing 1, 3, and 5 mol% Fe 2 O 3 , respectively, whereas the activation energies of the Fe/ZrSiO 4 formulation are 271.9, 261.9, and 257.7 kJ/mol, respectively. The parameter of growth morphology (n) and index of crystallization (m) were approximated as 2.0 and 1.0, respectively, meaning that two-dimensional growth with plate-like morphology was the primary mechanism of ZrO 2 crystallization from ZrO 2 –SiO 2 –Fe 2 O 3 precursor powders. The XRD results show that when the precursor powders of ZrO 2 –SiO 2 –1 mol

  5. Salmon on the Edge: Growth and Condition of Juvenile Chum and Pink Salmon in the Northeastern Bering Sea

    Science.gov (United States)

    McPhee, M. V.

    2016-02-01

    As the Arctic and Subarctic regions warm, Pacific salmon (Oncorhynchus spp.) are expected to expand their range northward during ice-free periods in the Bering and Chukchi seas. The oscillating control hypothesis, which describes energetic differences of primary consumers between ice-associated and pelagic production phases, provides a framework for understanding how juvenile salmon might respond to changing conditions at the northern edge of their marine range. Additionally, relationships between growth/condition and temperature, salinity and bottom depth will help identify marine habitats supporting growth at the Arctic-Subarctic interface. In this study, we used survey data from NOAA and Arctic Ecosystem Integrated Survey project to 1) compare growth and condition of juvenile pink (O. gorbuscha) and chum (O. keta) salmon in the NE Bering Sea between warm and cool spring phases, and 2) describe relationships between summer environmental conditions and juvenile salmon growth and condition from 2006 - 2010. Chum and pink salmon were shorter, and chum salmon exhibited greater energy density, in years with cool springs; however, no other aspects of size and condition differed significantly between phases. Over all years, longer and more energy dense individuals of both species were caught at stations with greater bottom depths and in cooler sea-surface temperatures. We found little evidence that chlorophyll-a explained much of the variation in size or condition. We used insulin-like growth factor-1 (IGF-1) concentration as an indicator of relative growth rate for fishes sampled in 2009-2012 and that found juvenile salmon exhibited higher IGF-1 concentrations in 2010-2012 than in 2009. IGF-1 concentrations tended to increase with SST in chum salmon and with bottom depth (a proxy for distance from shore) in pink salmon, but more years of data are needed to adequately describe the relationship of IGF with environmental conditions. This study, although descriptive in

  6. Pink slimed: Media framing of novel food technologies and risk related to ground beef and processed foods in the U.S.

    Science.gov (United States)

    Runge, Kristin K; Chung, Jennifer H; Su, Leona Yi-Fan; Brossard, Dominique; Scheufele, Dietram A

    2018-09-01

    In March 2012 ABC World News Report aired a series of reports on lean finely textured beef (LFTB) that resulted in a 10-year low for beef prices and the bankruptcy of a major firm that produced LFTB. Using a random sample survey, we tested the effects of the media frame "pink slime" and industry frame "lean finely textured beef," alongside media use, food-related knowledge, trust in food-related institutions and preference for local, fresh, organic and GMO-free foods on perceptions of risk related to ground beef containing pink slime/LFTB, processed foods and red meat. The "pink slime" frame was strongly and positively associated with risk related to ground beef, but not risk related to red meat or processed foods. Attention to news stories about pink slime/LFTB was strongly associated with risk related to ground beef and processed foods, but not red meat. We found varying effects of food values, knowledge and trust on all three dependent variables. Implications are discussed. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Climate change, pink salmon, and the nexus between bottom-up and top-down forcing in the subarctic Pacific Ocean and Bering Sea.

    Science.gov (United States)

    Springer, Alan M; van Vliet, Gus B

    2014-05-06

    Climate change in the last century was associated with spectacular growth of many wild Pacific salmon stocks in the North Pacific Ocean and Bering Sea, apparently through bottom-up forcing linking meteorology to ocean physics, water temperature, and plankton production. One species in particular, pink salmon, became so numerous by the 1990s that they began to dominate other species of salmon for prey resources and to exert top-down control in the open ocean ecosystem. Information from long-term monitoring of seabirds in the Aleutian Islands and Bering Sea reveals that the sphere of influence of pink salmon is much larger than previously known. Seabirds, pink salmon, other species of salmon, and by extension other higher-order predators, are tightly linked ecologically and must be included in international management and conservation policies for sustaining all species that compete for common, finite resource pools. These data further emphasize that the unique 2-y cycle in abundance of pink salmon drives interannual shifts between two alternate states of a complex marine ecosystem.

  8. Suspected natural lysosomal storage disease from ingestion of pink morning glory (Ipomoea carnea) in goats in northern Argentina.

    Science.gov (United States)

    Ríos, Elvio E; Cholich, Luciana A; Chileski, Gabriela; García, Enrique N; Lértora, Javier; Gimeno, Eduardo J; Guidi, María G; Mussart, Norma; Teibler, Gladys P

    2015-07-01

    This study describes an occurrence of pink morning glory (Ipomoea carnea) intoxication in goats in northern Argentina. The clinical signs displayed by the affected animals were ataxia, lethargy, emaciation, hypertonia of the neck muscles, spastic paresis in the hind legs, abnormal postural reactions and death. The clinico-pathologic examination revealed that the affected animals were anemic and their serum level of aspartate aminotransferase was significantly increased. Cytoplasmic vacuolation in the Purkinje cells and pancreatic acinar cells was observed by histological examination. The neuronal lectin binding pattern showed a strong positive reaction to WGA (Triticum vulgaris), sWGA (succinylated T. vulgaris) and LCA (Lens culinaris). Although I. carnea is common in tropical regions, this is the first report of spontaneous poisoning in goats in Argentina.

  9. Early Vessels Exploration of Pink Pulseless Hand in Gartland III Supracondylar Fracture Humerus in Children: Facts and Controversies

    Directory of Open Access Journals (Sweden)

    Tunku-Naziha TZ

    2017-03-01

    Full Text Available The management of pink pulseless limbs in supracondylar fractures has remained controversial, especially with regards to the indication for exploration in a clinically well-perfused hand. We reviewed a series of seven patients who underwent surgical exploration of the brachial artery following supracondylar fracture. All patients had a non-palpable radial artery, which was confirmed by Doppler ultrasound. CT angiography revealed complete blockage of the artery with good collateral and distal run-off. Two patients were more complicated with peripheral nerve injuries, one median nerve and one ulnar nerve. Only one patient had persistent arterial constriction which required reverse saphenous graft. The brachial arteries were found to be compressed by fracture fragments, but were in continuity. The vessels were patent after the release of obstruction and the stabilization of the fracture. There was no transection of major nerves. The radial pulse was persistently present after 12 weeks, and the nerve activity returned to full function.

  10. Diurnal Variation In Behaviour Of Pink-Footed Geese (Anser Brachyrhynchus) During Spring Migration In Trøndelag, Norway

    DEFF Research Database (Denmark)

    Chudzińska, Magda Ewa; Madsen, Jesper; Nabe-Nielsen, Jacob

    During spring migration, Pink-footed Geese Anser brachyrhynchus stop in mid Norway to refuel before their onward flight to the Svalbard breeding grounds. In mid Norway, geese feed on pastures, stubble as well as newly sown grain fields. The aim of the paper is to describe diurnal variations...... in the behaviour of geese and to examine whether these variations are driven by digestibility of food geese feed on or also by external factors such as distance to the roost, disturbance and flock size. Based on diurnal flock scans of activity budgets (observations carried out between 05h00 and 22h00 hrs) in each...... habitat type, we fitted a model containing all predictors we believe may influence geese behaviour. The number of feeding and alert geese on fields displayed a strong diurnal trend, which varied among habitat types, frequent and sporadic disturbance, but not flock size. On roost sites, geese also showed...

  11. Pink-pigmented non-fermentative gram-negative rods associated with human infections: a clinical and diagnostic challenge.

    Science.gov (United States)

    Hogue, R; Graves, M; Moler, S; Janda, J M

    2007-06-01

    Over the past several decades, the appearance of pink-pigmented bacteria in clinical specimens has gone from being a microbiologic curiosity in the clinical laboratory to the recognition of these aerobic microorganisms as etiologic agents of human disease, most notably bloodstream infections. Advances in the fields of molecular taxonomy and phylogenetics indicate that at least four distinct genera and eight different species are associated with clinical infections in susceptible patient populations. However, these bacteria are slow growing and present multiple diagnostic challenges to the microbiology laboratory including culture, isolation, and identification to species rank. This article provides a current review of these unusual non-fermentative chromogenic bacteria including their disease spectrum, taxonomy, and laboratory identification. The review also highlights the pitfalls or shortcomings we currently have in our knowledge of these microbes and their disease-producing capabilities.

  12. Ameliorating mitochondrial dysfunction restores carbon ion-induced cognitive deficits via co-activation of NRF2 and PINK1 signaling pathway.

    Science.gov (United States)

    Liu, Yang; Yan, Jiawei; Sun, Cao; Li, Guo; Li, Sirui; Zhang, Luwei; Di, Cuixia; Gan, Lu; Wang, Yupei; Zhou, Rong; Si, Jing; Zhang, Hong

    2018-07-01

    Carbon ion therapy is a promising modality in radiotherapy to treat tumors, however, a potential risk of induction of late normal tissue damage should still be investigated and protected. The aim of the present study was to explore the long-term cognitive deficits provoked by a high-linear energy transfer (high-LET) carbon ions in mice by targeting to hippocampus which plays a crucial role in memory and learning. Our data showed that, one month after 4 Gy carbon ion exposure, carbon ion irradiation conspicuously resulted in the impaired cognitive performance, neurodegeneration and neuronal cell death, as well as the reduced mitochondrial integrity, the disrupted activities of tricarboxylic acid cycle flux and electron transport chain, and the depressed antioxidant defense system, consequently leading to a decline of ATP production and persistent oxidative damage in the hippocampus region. Mechanistically, we demonstrated the disruptions of mitochondrial homeostasis and redox balance typically characterized by the disordered mitochondrial dynamics, mitophagy and glutathione redox couple, which is closely associated with the inhibitions of PINK1 and NRF2 signaling pathway as the key regulators of molecular responses in the context of neurotoxicity and neurodegenerative disorders. Most importantly, we found that administration with melatonin as a mitochondria-targeted antioxidant promoted the PINK1 accumulation on the mitochondrial membrane, and augmented the NRF2 accumulation and translocation. Moreover, melatonin pronouncedly enhanced the molecular interplay between NRF2 and PINK1. Furthermore, in the mouse hippocampal neuronal cells, overexpression of NRF2/PINK1 strikingly protected the hippocampal neurons from carbon ion-elicited toxic insults. Thus, these data suggest that alleviation of the sustained mitochondrial dysfunction and oxidative stress through co-modulation of NRF2 and PINK1 may be in charge of restoration of the cognitive impairments in a mouse

  13. Marine reserves help preserve genetic diversity after impacts derived from climate variability: Lessons from the pink abalone in Baja California

    Directory of Open Access Journals (Sweden)

    Adrián Munguía-Vega

    2015-07-01

    Full Text Available Genetic diversity is crucial for the adaptation of exploited species like the pink abalone (Haliotis corrugata, faced with threats from climate change, overfishing and impacts associated with aquaculture production. While marine reserves are commonly used to mitigate risks to marine populations, the duration, size, location and larval connectivity needed for a reserve to help conserve genetic resources is still poorly understood. Here, we examine the effects of fishing, reserves, and restocking on the genetic diversity of 10 populations from central Baja California, Mexico, and Southern California, USA. We demonstrate that each population shows characteristic genetic signatures according to recent management decisions. We found high allelic diversity, particularly rare alleles, a larger effective population size and a lack of a recent genetic bottleneck in pink abalones within a small (0.8 km2, recently established (5 years reserve in Baja California, compared to other fished sites after a climatic bottleneck. Higher diversity may result from the presence of older animals in the reserve. Due to its location, the reserve may also act as an important hub connecting distant populations via larval dispersal. In contrast, a population from California showed genetic isolation, loss of allelic diversity and high relatedness, consistent with the collapse of fisheries in the 1990s and their lack of recovery thereafter. In addition, a fished area in Baja California with a history of restocking for over a decade showed an increase in frequency of related individuals and high genetic differentiation from nearby sites that were consistent with the production of larvae from a few adults in the laboratory. A network of strategically placed small marine reserves that considers ocean circulation patterns could help to maintain genetic diversity and connectivity of exploited populations.

  14. Degradation of formaldehyde at high concentrations by phenol-adapted Ralstonia eutropha closely related to pink-pigmented facultative methylotrophs.

    Science.gov (United States)

    Habibi, Alireza; Vahabzadeh, Farzaneh

    2013-01-01

    The ability of the phenol-adapted Ralstonia eutropha to utilize formaldehyde (FD) as the sole source of carbon and energy was studied. Adaptation to FD was accomplished by substituting FD for glucose in a stepwise manner. The bacterium in the liquid test culture could tolerate concentrations of FD up to 900 mg L(-1). Degradation of FD was complete in 528 h at 30°C with shaking at 150 rpm (r = 1.67 mg L(-1) h(-1)), q = 0.035 g(FD) g(cell) (-1) h(-1). Substrate inhibition kinetics (Haldane and Luong equations) are used to describe the experimental data. At non-inhibitory concentrations of FD, the Monod equation was used. According to the Luong model, the values of the maximum specific growth rate (μ(max)), half-saturation coefficient (k(S)), the maximum allowable formaldehyde concentration (S(m)), and the shape factor (n) were 0.117 h(-1), 47.6 mg L(-1), 900 mg L(-1), and 2.2, respectively. The growth response of the test bacterium to consecutive FD feedings was examined, and the FD-adapted R. eutropha cells were able to degrade 1000 mg L(-1) FD in 150 h through 4 cycles of FD feeds. During FD degradation, formic acid metabolite was formed. Assimilation of FD, methanol, formic acid, and oxalate by the test bacterium was accompanied by the formation of a pink pigment. The carotenoid nature of the cellular pigment has been confirmed and the test bacterium appeared to be closely related to pink-pigmented facultative methylotrophs (PPFM). The extent of harm to soil exposed to biotreated wastewaters containing FD may be moderated due to the association between methylotrophic/oxalotrophic bacteria and plants.

  15. Control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic Delftia lacustris and Bacillus spp.

    Directory of Open Access Journals (Sweden)

    Veeranan Janahiraman

    2016-11-01

    Full Text Available The studies on the biocontrol potential of pink pigmented facultative methylotrophic (PPFM bacteria other than the genus Methylobacterium are scarce. In the present study, we report three facultative methylotrophic isolates; PPO-1, PPT-1 and PPB-1, respectively identified as Delftia lacustris, Bacillus subtilis and Bacillus cereus by 16S rRNA gene sequence analysis. Hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicates that the isolates PPO-1, PPT-1 and PPB-1 are not pathogenic strains. Under in vitro conditions, D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 showed direct antagonistic effect by inhibiting the mycelial growth of fungal pathogens; Fusarium oxysporum f. sp. lycopersici (2.15, 2.05 and 1.95 cm, Sclerotium rolfsii (2.14, 2.04 and1.94 cm, Pythium ultimum (2.12, 2.02 and 1.92cm, and Rhizoctonia solani (2.18, 2.08 and 1.98 cm and also produced volatile inhibitory compounds. Under plant growth chamber condition methylotrophic bacterial isolates; D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 significantly reduced the disease incidence of tomato. Under greenhouse condition, D. lacustris PPO-1, B. subtilis PPT-1 and B. cereus PPB-1 inoculated tomato plants, when challenged with F. oxysporum f. sp. lycopersici, S. rolfsii, P. ultimum and R. solani, increased the pathogenesis related proteins (β-1, 3-glucanase and chitinase and defense enzymes (phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, and catalase on day 5 after inoculation. In the current study, we first report the facultative methylotrophy in pink pigmented Delftia lacustris, B. subtilis, and B. cereus and their antagonistic potential against fungal pathogens. Direct antagonistic and ISR effects of these isolates against fungal pathogens of tomato evidenced their possible use as a biocontrol agent.

  16. Kinetics of phase transformation and optical property of pink coral zirconia powders

    Energy Technology Data Exchange (ETDEWEB)

    Chu, Hsueh-Liang; Wang, Cheng-Li [Department of Materials Science and Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Hwang, Weng-Sing [Department of Materials Science and Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University, 1 Ta-Hsueh Road, Tainan 70101, Taiwan (China); Lee, Kuen-Chan, E-mail: kclee@kmu.edu.tw [Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan (China); Zhou, Xuedong [State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070 (China); Wang, Moo-Chin, E-mail: mcwang@kmu.edu.tw [Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan (China)

    2014-07-15

    Highlights: • The single phase of tetragonal ZrO{sub 2} formed when calcined at 1223 K for 1 h. • The tetragonal ZrO{sub 2} fully converted to ZrSiO{sub 4} when calcined at 1323–1473 K for 1 h. • The activation energy of t-ZrO{sub 2} formed is 399.9 kJ/mol when 5 mol% Fe{sub 2}O{sub 3} added. • The activation energy of the Fe/ZrSiO{sub 4} formed is 257.7 kJ/mol when 5 mol% Fe{sub 2}O{sub 3} added. • The growth morphology parameter and crystallization index are about 2.0 and 1.0. - Abstract: The kinetics of phase transformation and optical property of pink coral zircon powders have been studied. The ZrO{sub 2}–SiO{sub 2}–Fe{sub 2}O{sub 3} precursor powders were synthesized using Zr(NO{sub 3}){sub 4}⋅4H{sub 2}O, Si(C{sub 2}H{sub 5}O){sub 4} and Fe(NO{sub 3}){sub 3}⋅9H{sub 2}O as initial materials via the hot–wet routes. The kinetics of phase transformation of the ZrO{sub 2}–SiO{sub 2}–Fe{sub 2}O{sub 3} precursor powders was characterized by thermo-gravimetric (TG)/differential scanning calorimeter (DSC), X-ray diffraction (XRD), transmission electron microscopy (TEM), nano-beam electron diffraction (NBED), and spectrophotometry. The crystallization temperatures of tetragonal zirconia (t-ZrO{sub 2}) and zircon (ZrSiO{sub 4}) of ZrO{sub 2}–SiO{sub 2} precursor powders with 1 mol% Fe{sub 2}O{sub 3} were estimated to be approximately 1204 K and 1496 K, respectively, based on a DSC analysis conducted at a heating rate of 20 K/min. The activation energies of t-ZrO{sub 2} formulation are 428.2, 403.2, and 399.9 kJ/mol, respectively, for ZrO{sub 2}–SiO{sub 2} precursor powders containing 1, 3, and 5 mol% Fe{sub 2}O{sub 3}, respectively, whereas the activation energies of the Fe/ZrSiO{sub 4} formulation are 271.9, 261.9, and 257.7 kJ/mol, respectively. The parameter of growth morphology (n) and index of crystallization (m) were approximated as 2.0 and 1.0, respectively, meaning that two-dimensional growth with plate-like morphology was the

  17. Biology of Pink Hibiscus MealybugMaconellicoccushirsutus (Green on Chinese Hibiscus Shrubs in Khuzestan Province

    Directory of Open Access Journals (Sweden)

    M. Sadat Alizadeh

    2017-12-01

    Full Text Available Introduction: The pink hibiscus mealybug, Maconellicoccushirsutus (Green (Hem.:Pseudococcidae is one of the serious economic pests of agricultural crops in tropical and subtropical regions.This pesthas spread through Asia, Africa, America and Australia, and infests more than 330 host plants which some of them have the important role in theeconomy. This sap-feeding insect secrets honeydew which provides suitablemedium for black sooty moldgrowth. It also releases a toxin during feeding, which results in severe stunting, decline, and deformation of twigs. It has recorded as a pest on different host plants in southern Iranian provinces since the 1990s.In Khuzestan province, it was first observed on Chinese hibiscus shrubs in urban landscapes of Ahvaz in February 2009. The evergreen hibiscus shrub is very common in the urban landscape of warmcities of Khuzestan province. The severity of mealybug damage to hibiscus shrubs causedto becut off in some regions. As there is no data on the biology of M. hirsutus in Iran,in the present study, the biologyof this pestwas investigatedat different temperatures in laboratory conditions as well as its population fluctuations on Chinese hibiscus shrubs in urban landscapes of Ahvaz. Materials and methods: Biology of the pest species was evaluated at five laboratory constant temperatures 15, 20, 25, 30and 35±2°C (65±5% RH and a photoperiod of L: D 14:10 h on Chinese hibiscus leaves in plastic boxes (8×6×2 cm. Number ofM. hirsutusgenerations was also studied in clip cages on hibiscus shrubs in Ahvaz urban landscapes within a year. Population fluctuations of the insect were also investigated on those shrubs in two different regions of Ahvaz for 16 months. For this purpose, random sampling was done on five shrubs in each region bi-weekly. Eight twigs, 5 cm in length, were cut from the upper and lower halves of each shrub quadrant and a number of all mealybug developmental stages per twig were counted and recorded

  18. [Density, size structure and reproductive activity of the pink conch Eustrombus gigas (Mesogastropoda: Strombidae) in Banco Chinchorro, Mexico].

    Science.gov (United States)

    Cala, Yuself R; Navarrete, Alberto de Jesús; Ocaña, Frank A; Rivera, José Oliva

    2013-12-01

    The pink conch Eustrombus gigas is an important fisheries resource. At the regional level in the Caribbean, over-exploitation and habitat destruction have caused a decrease in the abundance of this resource. In order to provide necessary information for the species management in Mexico, this work aimed to analyze the total density, adult density, size structure and reproductive behavior of pink conch population at Banco Chinchorro during 2009-2010. Data from three seasons were obtained (rainy, dry and cold fronts periods) in three areas: Norte (North), Centro (Center) and Sur (South). The organisms were separated into two groups: (a) the criteria based upon legal harvest in Mexico: legal size conchs (siphonal length > 200 mm) and illegal size conchs (siphonal length sexual maturity using the 15 mm lip thickness standard: lip or = 15 mm as adult conch. Copulation, spawning, egg masses and aggregations were evaluated as reproductive evidences. The highest total density was observed during the dry season with 384ind./ha, and the lowest during the rainy season with 127ind./ha. The highest density was reported at Sur (385ind./ha) and the lowest at Norte (198ind./ ha). The highest adult density was observed during the rainy season (8.33ind./ha), and the lowest occurred in the dry season (6.1 ind./ha). Adult density values were 5.55, 7.05 and 8.33ind./ha for Centro, Sur and Norte areas, respectively. Adult densities were lower than the threshold needed for reproduction, and 42% of the population may be vulnerable to fishing, as they had the minimum size for catch (Lsi 200 mm). Furthermore, only 2.2% of the population reached a Gl > 15 mm as sexual maturity indicator. During the study period, only six evidences of reproductive activity were observed. The smaller densities reported at Banco Chinchorro may cause reproduction events to be almost absent which in turn is sufficient evidence to show that the Allee Effect is acting on the queen conch population and there is an

  19. Composition of the Essential Oil of Pink Chablis Bluebeard (Caryopteris x clandonensis ’Durio’) and Its Biological Activity against the Yellow Fever Mosquito Aedes aegypti

    Science.gov (United States)

    2016-04-07

    oil of Pink Chablis™ bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti ARTICLE...bluebeard (Caryopteris ×clandonensis ’Durio’) and its biological activity against the yellow fever mosquito Aedes aegypti Eugene K. Blythe1...mosquito [ Aedes aegypti (L.) (Diptera: Culicidae)]. Essential oil from the aerial parts of this mildly aromatic ornamental species was extracted by water

  20. The Abundance of Pink-Pigmented Facultative Methylotrophs in the Root Zone of Plant Species in Invaded Coastal Sage Scrub Habitat

    OpenAIRE

    Irvine, Irina C.; Brigham, Christy A.; Suding, Katharine N.; Martiny, Jennifer H.

    2012-01-01

    Pink-pigmented facultative methylotrophic bacteria (PPFMs) are associated with the roots, leaves and seeds of most terrestrial plants and utilize volatile C(1) compounds such as methanol generated by growing plants during cell division. PPFMs have been well studied in agricultural systems due to their importance in crop seed germination, yield, pathogen resistance and drought stress tolerance. In contrast, little is known about the PPFM abundance and diversity in natural ecosystems, let alone...

  1. Pink spot, white spot: the pineal skylight of the leatherback turtle (Dermochelys coriacea Vandelli 1761) skull and its possible role in the phenology of feeding migrations

    Science.gov (United States)

    Davenport, John; Jones, T. Todd; Work, Thierry M.; Balazs, George H.

    2014-01-01

    Leatherback turtles, Dermochelys coriacea, which have an irregular pink area on the crown of the head known as the pineal or ‘pink spot’, forage upon jellyfish in cool temperate waters along the western and eastern margins of the North Atlantic during the summer. Our study showed that the skeletal structures underlying the pink spot in juvenile and adult turtles are compatible with the idea of a pineal dosimeter function that would support recognition of environmental light stimuli. We interrogated an extensive turtle sightings database to elucidate the phenology of leatherback foraging during summer months around Great Britain and Ireland and compared the sightings with historical data for sea surface temperatures and day lengths to assess whether sea surface temperature or light periodicity/levels were likely abiotic triggers prompting foraging turtles to turn south and leave their feeding grounds at the end of the summer. We found that sea temperature was too variable and slow changing in the study area to be useful as a trigger and suggest that shortening of day lengths as the late summer equilux is approached provides a credible phenological cue, acting via the pineal, for leatherbacks to leave their foraging areas whether they are feeding close to Nova Scotia or Great Britain and Ireland.

  2. The influence of EDTA application on the interactions of cadmium, zinc, and lead and their uptake of rainbow pink (Dianthus chinensis).

    Science.gov (United States)

    Lai, Hung-Yu; Chen, Zueng-Sang

    2006-10-11

    Soil used in this study was artificially contaminated with Cd, Zn, Pb, or applied in combinations (Cd-Zn, Cd-Pb, Zn-Pb, or Cd-Zn-Pb) to study the interactions of metals in soil contaminated with multiple metals. After planting rainbow pink (Dianthus chinensis) in these soils for 21 days, three different concentrations of ethylenedinitrilotetraacetic acid (EDTA) solutions were added to study the effect of applying EDTA on the interactions among these metals. The concentrations of Cd, Zn, and Pb in the soil solutions of different metals-treated soils increased significantly after applying 5 mmol EDTA kg(-1) soil (p<0.05). The potential of groundwater contamination will increase after applying EDTA and it is not recommended to be in situ used or have to use very carefully. The existence of Pb in the Cd-contaminated soil enhanced the uptake of Cd in rainbow pink in the treatments of control and 2 mmol EDTA kg(-1) soil. Cadmium inhibited the concentration of Zn without applying EDTA. However, whether the application of EDTA or not and the applied EDTA concentration had the greatest effect on the uptake of Pb when compared to Cd and Zn. After applying 5 mmol EDTA kg(-1) soil, Cd or Zn in the Pb-contaminated soil inhibited the uptake of Pb in rainbow pink, but there were no effect in other treatments.

  3. Near-infrared 808 nm light boosts complex IV-dependent respiration and rescues a Parkinson-related pink1 model.

    Directory of Open Access Journals (Sweden)

    Melissa Vos

    Full Text Available Mitochondrial electron transport chain (ETC defects are observed in Parkinson's disease (PD patients and in PD fly- and mouse-models; however it remains to be tested if acute improvement of ETC function alleviates PD-relevant defects. We tested the hypothesis that 808 nm infrared light that effectively penetrates tissues rescues pink1 mutants. We show that irradiating isolated fly or mouse mitochondria with 808 nm light that is absorbed by ETC-Complex IV acutely improves Complex IV-dependent oxygen consumption and ATP production, a feature that is wavelength-specific. Irradiating Drosophila pink1 mutants using a single dose of 808 nm light results in a rescue of major systemic and mitochondrial defects. Time-course experiments indicate mitochondrial membrane potential defects are rescued prior to mitochondrial morphological defects, also in dopaminergic neurons, suggesting mitochondrial functional defects precede mitochondrial swelling. Thus, our data indicate that improvement of mitochondrial function using infrared light stimulation is a viable strategy to alleviate pink1-related defects.

  4. Assessment of trophic transfer of benzo(a)pyrene genotoxicity from the post-larval pink shrimp F. brasiliensis to the juvenile Florida pompano T. carolinus.

    Science.gov (United States)

    Rocha, Arthur José da Silva; Santos, Thaís Cruz Alves; Gomes, Vicente; Bícego, Márcia Caruso; Barbosa, Ana Cecília Rizzatti de Albergaria; Passos, Maria José de Arruda Campos Rocha; Hasue, Fabio Matsu; Van Ngan, Phan

    2012-11-01

    In the present study, the polycyclic aromatic hydrocarbon (PAH) genotoxicity was investigated in a one-step predator-prey relationship with the trophic-related marine species. Florida pompanos were fed for 5 and 10 days with pink shrimp post larvae previously exposed to benzo(a)pyrene (BaP) concentrations. Parent BaP body burden was measured in samples of Farfantepenaeus brasiliensis. BaP metabolites were determined in bile samples of Trachinotus carolinus and DNA damage was assessed through the comet and erythrocyte nuclear abnormalities (ENAs) assays in fish erythrocytes. BaP body burden increased significantly with the PAH concentration in pink shrimp PLs as well as the fish bile BaP metabolites. Both, comet and ENAs assays indicated significant increase on erythrocyte DNA damage of Florida pompanos fed with BaP-exposed pink shrimp on both feeding periods. The trophic route of BaP genotoxicity is discussed as well as the PAH biotransformation as the inducing mechanism for the DNA damages observed. Copyright © 2012 Elsevier B.V. All rights reserved.

  5. A resource management scenario for traditional and scientific management of pink shrimp (Farfantepenaeus paulensis) in the Patos Lagoon estuary (RS), Brazil.

    Science.gov (United States)

    Moura, Gustavo Goulart Moreira; Kalikoski, Daniela Coswig; Diegues, Antonio Carlos Sant'Ana

    2013-01-11

    This article aims to discuss the incorporation of traditional time in the construction of a management scenario for pink shrimp in the Patos Lagoon estuary (RS), Brazil. To meet this objective, two procedures have been adopted; one at a conceptual level and another at a methodological level. At the conceptual level, the concept of traditional time as a form of traditional ecological knowledge (TEK) was adopted. At the methodological level, we conduct a wide literature review of the scientific knowledge (SK) that guides recommendations for pink shrimp management by restricting the fishing season in the Patos Lagoon estuary; in addition, we review the ethno-scientific literature which describes traditional calendars as a management base for artisanal fishers in the Patos Lagoon estuary. Results demonstrate that TEK and SK describe similar estuarine biological processes, but are incommensurable at a resource management level. On the other hand, the construction of a "management scenario" for pink shrimp is possible through the development of "criteria for hierarchies of validity" which arise from a productive dialog between SK and TEK. The commensurable and the incommensurable levels reveal different basis of time-space perceptions between traditional ecological knowledge and scientific knowledge. Despite incommensurability at the management level, it is possible to establish guidelines for the construction of "management scenarios" and to support a co-management process.

  6. Relationship between growth and total nucleic acids in juvenile pink salmon, Oncorhynchus gorbuscha, fed crude oil contaminated food

    International Nuclear Information System (INIS)

    Wang Shiao, Y.; Lum, J.L.; Carls, M.G.; Rice, S.D.

    1993-01-01

    Total nucleic acids of junvenile pink salmon fed crude oil contaminated food were analyzed to deteremine if nucleic acid measurements can be used to evaluate growth of fish collected at oil spill sites. In general, the nucleic acid concentration (μg per mg dry weight) of salmon fry fed food contaminated with either 0.37 or 2.78 mg crude oil/g food was not significantly affected. However, RNA concentration of fry fed food contaminated with 34.83 mg/g was reduced whereas DNA concentration increased. Results over 8 weeks indicate decreased protein synthesis and cell content but maintenance of cell integrity in these fish. Growth was inversely related to the level of crude oil contamination in the food. The significant correlations between measured growth and RNA/DNA ratios and RNA contents (mg RNA per mm fork length) suggest that nucleic acid measurement can be used to compare growth of fish collected from the field. 23 refs., 4 figs

  7. A fixed angle double mirror filter for preparing a pink undulator beam at the Advanced Photon Source

    International Nuclear Information System (INIS)

    Dufresne, E.; Sanchez, T.; Nurushev, T.; Clarke, R.; Dierker, S.B.

    2000-01-01

    Recent advances in X-ray Photon Correlation Spectroscopy (XPCS) use the full bandwidth of an undulator harmonic in order to maximize the coherent flux for small angle X-ray scattering experiments. X-ray mirrors and filters are typically used to select a given harmonic of the spectrum. At the University of Michigan/Howard University/Lucent Technologies, Bell Labs, Collaborative Access Team (MHATT-CAT) undulator beamline of the Advanced Photon Source, we have designed a fixed-angle Double Mirror Filter which will provide a 'pink beam' (i.e., 2-3% bandwidth) for XPCS experiments. This device uses two small mirrors which vertically reflect a 0.1 mmx0.1 mm white beam in a symmetric geometry. The doubly reflected beam propagates parallel to the incident white beam, but is offset vertically by 35 mm. Using the standard offset of the APS allows one to stop the white beam with a standard APS beam stop. In this report, we will describe our design considerations for this instrument. We also report the results of preliminary tests of the performance. The mirrors preserve the transverse coherence of the source, and filter the undulator spectrum as expected

  8. Drosophila PINK1 and parkin loss-of-function mutants display a range of non-motor Parkinson's disease phenotypes.

    Science.gov (United States)

    Julienne, Hannah; Buhl, Edgar; Leslie, David S; Hodge, James J L

    2017-08-01

    Parkinson's disease (PD) is more commonly associated with its motor symptoms and the related degeneration of dopamine (DA) neurons. However, it is becoming increasingly clear that PD patients also display a wide range of non-motor symptoms, including memory deficits and disruptions of their sleep-wake cycles. These have a large impact on their quality of life, and often precede the onset of motor symptoms, but their etiology is poorly understood. The fruit fly Drosophila has already been successfully used to model PD, and has been used extensively to study relevant non-motor behaviours in other contexts, but little attention has yet been paid to modelling non-motor symptoms of PD in this genetically tractable organism. We examined memory performance and circadian rhythms in flies with loss-of-function mutations in two PD genes: PINK1 and parkin. We found learning and memory abnormalities in both mutant genotypes, as well as a weakening of circadian rhythms that is underpinned by electrophysiological changes in clock neurons. Our study paves the way for further work that may help us understand the mechanisms underlying these neglected aspects of PD, thus identifying new targets for treatments to address these non-motor problems specifically and perhaps even to halt disease progression in its prodromal phase. Crown Copyright © 2017. Published by Elsevier Inc. All rights reserved.

  9. Methylobacterium marchantiae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the thallus of a liverwort.

    Science.gov (United States)

    Schauer, S; Kämpfer, P; Wellner, S; Spröer, C; Kutschera, U

    2011-04-01

    A pink-pigmented, facultatively methylotrophic bacterium, designated strain JT1(T), was isolated from a thallus of the liverwort Marchantia polymorpha L. and was analysed by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis placed the strain in a clade with Methylobacterium adhaesivum AR27(T), Methylobacterium fujisawaense DSM 5686(T), Methylobacterium radiotolerans JCM 2831(T) and Methylobacterium jeotgali S2R03-9(T), with which it showed sequence similarities of 97.8, 97.7, 97.2 and 97.4 %, respectively. However, levels of DNA-DNA relatedness between strain JT1(T) and these and the type strains of other closely related species were lower than 70 %. Cells of JT1(T) stained Gram-negative and were motile, rod-shaped and characterized by numerous fimbriae-like appendages on the outer surface of their wall (density up to 200 µm(-2)). Major fatty acids were C(18 : 1)ω7c and C(16 : 0). Based on the morphological, physiological and biochemical data presented, strain JT1(T) is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium marchantiae sp. nov. is proposed. The type strain is JT1(T) ( = DSM 21328(T)  = CCUG 56108(T)).

  10. Characteristics of a pink-pigmented bacterium isolated from biofilm in a cooling tower in Tokyo, Japan.

    Science.gov (United States)

    Furuhata, Katsunori; Goto, Keiichi; Kato, Yuko; Saitou, Keiko; Sugiyama, Jun-ichi; Hara, Motonobu; Yoshida, Shin-ichi; Fukuyama, Masafumi

    2007-01-01

    Strain K-20, a Gram-negative, non-motile, non-spore-forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in Tokyo, Japan. The taxonomic feature of the strain was studied using phenotypic tests and phylogenetic analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was related to Roseomonas gilardii subsp. rosea, Roseomonas gilardii subsp. gilardii, Roseomonas cervicalis and Roseomonas mucosa at 94.3-94.6 sequence similarities. Growth occurred at 25-40 C and pH 5.0-10.0, optimal at 35 C and pH 7.0. Growth did not occur in the presence of >or=2% NaCl. The API 20NE identification system gave a positive result for urease, L-arabinose, potassium gluconate, adipic acid, malic acid and trisodium citrate (API code number 0201465). The predominant fatty acids of strain K-20 were C18:1Delta11 (50.8%) and C16:1 (17.2%). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 72.0 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data, it was assumed that strain K-20 (=JCM 14634) is a novel species of the genus Roseomonas.

  11. Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China.

    Science.gov (United States)

    He, Y B; Zhan, R L; Sun, G M; Wu, J B; Zhao, Y L

    2015-08-19

    The pink pineapple mealybug (PPM), Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. Its distribution is closely linked with its host, the pineapple [Ananas comosus (L.) Merrill] because of its wingless and parthenogenetic characteristics. To investigate the history of D. brevipes introduction and the cultivation of pineapple in China, samples of D. brevipes were collected from the main pineapple production region in China, and from Thailand, and the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed. Homologous sequences of D. brevipes COI from Brazil, Thailand, and Philippines that are deposited in GenBank were compared. Phylogenetic analyses suggest there are close genetic relationships between PPM populations from Hawaii, Brazil, the Philippines, and from Thailand and China, which probably originate from South America. It is suggested that most PPMs in China were introduced from South America by way of Southeast Asia, being accompanied by the pineapple seedling. Conversely, some PPMs represented by Haplotype-WN from Wanning of China, and Lampang of Thailand were found to differ greatly from populations in Hawaii, Brazil, the Philippines, Thailand, and China. It is possible that another route was used for the introduction and distribution of pineapple, or that pineapple might have originated in Southeast Asia.

  12. Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone.

    Science.gov (United States)

    Meena, Kamlesh K; Kumar, Manish; Kalyuzhnaya, Marina G; Yandigeri, Mahesh S; Singh, Dhananjaya P; Saxena, Anil K; Arora, Dilip K

    2012-05-01

    Methylotrophic bacteria were isolated from the phyllosphere of different crop plants such as sugarcane, pigeonpea, mustard, potato and radish. The methylotrophic isolates were differentiated based on growth characteristics and colony morphology on methanol supplemented ammonium mineral salts medium. Amplification of the mxaF gene helped in the identification of the methylotrophic isolates as belonging to the genus Methylobacterium. Cell-free culture filtrates of these strains enhanced seed germination of wheat (Triticum aestivum) with highest values of 98.3% observed using Methylobacterium sp. (NC4). Highest values of seedling length and vigour were recorded with Methylobacterium sp. (NC28). HPLC analysis of production by bacterial strains ranged from 1.09 to 9.89 μg ml(-1) of cytokinins in the culture filtrate. Such cytokinin producing beneficial methylotrophs can be useful in developing bio-inoculants through co-inoculation of pink-pigmented facultative methylotrophs with other compatible bacterial strains, for improving plant growth and productivity, in an environment-friendly manner.

  13. PEMANFAATAN MINYAK BUAH MERAH, Pandanus conoideus Lam DAN CAROPHYLL PINK DALAM RANSUM PAKAN YUWANA IKAN KAKAP MERAH, Lutjanus sebae

    Directory of Open Access Journals (Sweden)

    Titiek Aslianti

    2016-11-01

    Full Text Available Ikan kakap merah, Lutjanus sebae termasuk komoditas budidaya yang banyak diminati konsumen. Kualitas benih untuk pembesaran selain ditentukan dari ukuran dan performansi fisik, juga berdasarkan warna ikan. Dalam penelitian ini telah dilakukan 4 perlakuan pakan yang diperkaya dengan Minyak Buah Merah (MBM dan Carophyll Pink (CP yaitu: (A 10 mL MBM/kg pakan; (B 10 mL MBM+1,4 g CP/kg pakan; (C 1,4 g CP/kg pakan, dan (D Kontrol. Hewan uji adalah yuwana kakap merah dengan rata-rata panjang dan bobot tubuh awal 11,32±0,76 cm dan 37,56±7,22 g. Ikan ditempatkan dalam bak fiber kapasitas 5 m3 dengan kepadatan 200 ekor/bak dan diberi pakan 2 kali sehari secara ad libitum serta dipelihara selama 2 bulan dengan pergantian air 200%-300%/hari. Kualitas yuwana diamati pada akhir penelitian melalui pengukuran panjang dan bobot tubuh, sedangkan performansi warna diamati dengan kamera digital dan dianalisis secara diskriptif menggunakan Microsoft Adobe Photoshop 8. Hasil penelitian menunjukkan bahwa MBM dan CP dapat dimanfaatkan dalam ransum pakan guna meningkatkan pertumbuhan dan performansi warna yuwana kakap merah. Pakan yang mengandung 10 mL MBM+1,4 g CP menghasilkan sintasan 94%, kenaikan pertumbuhan panjang dan bobot mutlak 58,92% dan 281%, konversi pakan 0,82 serta performansi yuwana berwarna merah cerah dengan nilai persentase rata-rata sebesar 89,58%±0,07%. Red emperor snapper, Lutjanus sebae is one of high demanded cultured fishes. The quality of seeds does not only depend on size and performance of fish, but also depends on fish color. A feeding experiment was conducted during two-months period by adding red fruit oil (RFO and carophyll pink (CP to every kilogram of pellet fed as the treatment i.e. (A 10 mL RFO/kg feed; (B 10 mL RFO+1.4 g CP/kg feed; (C 1.4 g CP /kg feed and (D Control. Two hundred seeds with initial total length of 11.32±0.76 cm and 37.56±7.22 g of body weight were stocked in a fiber glass tank of 5 m3. Feeding frequency was

  14. Molecular characterization and expression profiling of ryanodine receptor gene in the pink stem borer, Sesamia inferens (Walker).

    Science.gov (United States)

    Wu, Shun-Fan; Zhao, Dan-Dan; Huang, Jing-Mei; Zhao, Si-Qi; Zhou, Li-Qi; Gao, Cong-Fen

    2018-04-01

    The susceptibilities of three field populations of pink stem borer (PSB), Sesamia inferens (walker) to diamide insecticides, chlorantraniliprole and flubendiamide, were evaluated in this study. The results showed that these PSB field populations were still sensitive to the two diamide insecticides after many years of exposure. To further understand PSB and diamide insecticide, the full-length ryanodine receptor (RyR) cDNA (named as SiRyR), the molecular target of diamide insecticides was cloned from PSB and characterized. The SiRyR gene contains an open reading frame of 15,420 nucleotides, encoding 5140 amino acid residues, which shares 77% to 98% sequence identity with RyR homologous of other insects. All hallmarks of RyR proteins are conserved in the SiRyR protein, including the conserved C-terminal domain with the consensus calcium-biding EF-hands (calcium-binding motif), the six transmembrane domains, as well as mannosyltransferase, IP3R and RyR (pfam02815) (MIR) domains. Real-time qPCR analysis revealed that the highest mRNA expression levels of SiRyR were observed in pupa and adults, especially in males. SiRyR was expressed at the highest level in thorax, and the lowest level in wing. The full genetic characterization of SiRyR could provide useful information for future functional expression studies and for discovery of new insecticides with selective insecticidal activity. Copyright © 2018 Elsevier Inc. All rights reserved.

  15. Methylobacterium trifolii sp. nov. and Methylobacterium thuringiense sp. nov., methanol-utilizing, pink-pigmented bacteria isolated from leaf surfaces.

    Science.gov (United States)

    Wellner, S; Lodders, N; Glaeser, S P; Kämpfer, P

    2013-07-01

    Three pink-pigmented, aerobic, Gram-stain-negative, rod-shaped and facultatively methylotrophic strains were isolated from the phyllosphere of Trifolium repens and Cerastium holosteoides. 16S rRNA gene sequence analysis support the affiliation of all strains to the genus Methylobacterium. The closest relatives of strains C34(T) and T5 were Methylobacterium gnaphalii 23e(T) (98.0 and 98.5 % sequence similarity, respectively) and Methylobacterium organophilum JCM 2833(T) (97.0 and 97.2 %, respectively). Strain TA73(T) showed the highest sequence similarities to Methylobacterium marchantiae JT1(T) and Methylobacterium bullatum F3.2(T) (both 97.9 %), followed by Methylobacterium phyllosphaerae CBMB27(T) and Methylobacterium brachiatum DSM 19569(T) (both 97.8 %), Methylobacterium cerastii C15(T) and Methylobacterium radiotolerans JCM 2831(T) (both 97.7 %). The major components in the fatty acid profiles were C18 : 1ω7c, C16 : 0 and one unknown fatty acid for strain TA73(T) and C18 : 1ω7c, C16 : 1ω7c/iso-C15 : 0 2-OH, C18 : 0 and C16 : 0 for strains C34(T) and T5. Physiological and biochemical analysis, including DNA-DNA hybridization, revealed clear differences between the investigated strains and their closest phylogenetic neighbours. DNA-DNA hybridization studies also showed high similarities between strains C34(T) and T5 (59.6-100 %). Therefore, the isolates represent two novel species within the genus Methylobacterium, for which the names Methylobacterium trifolii sp. nov. (type strain TA73(T) = LMG 25778(T) = CCM 7786(T)) and Methylobacterium thuringiense sp. nov. (type strain C34(T) = LMG 25777(T) = CCM 7787(T)) are proposed.

  16. Preferences for Pink and Blue: The Development of Color Preferences as a Distinct Gender-Typed Behavior in Toddlers.

    Science.gov (United States)

    Wong, Wang I; Hines, Melissa

    2015-07-01

    Many gender differences are thought to result from interactions between inborn factors and sociocognitive processes that occur after birth. There is controversy, however, over the causes of gender-typed preferences for the colors pink and blue, with some viewing these preferences as arising solely from sociocognitive processes of gender development. We evaluated preferences for gender-typed colors, and compared them to gender-typed toy and activity preferences in 126 toddlers on two occasions separated by 6-8 months (at Time 1, M = 29 months; range 20-40). Color preferences were assessed using color cards and neutral toys in gender-typed colors. Gender-typed toy and activity preferences were assessed using a parent-report questionnaire, the Preschool Activities Inventory. Color preferences were also assessed for the toddlers' parents using color cards. A gender difference in color preferences was present between 2 and 3 years of age and strengthened near the third birthday, at which time it was large (d > 1). In contrast to their parents, toddlers' gender-typed color preferences were stronger and unstable. Gender-typed color preferences also appeared to establish later and were less stable than gender-typed toy and activity preferences. Gender-typed color preferences were largely uncorrelated with gender-typed toy and activity preferences. These results suggest that the factors influencing gender-typed color preferences and gender-typed toy and activity preferences differ in some respects. Our findings suggest that sociocognitive influences and play with gender-typed toys that happen to be made in gender-typed colors contribute to toddlers' gender-typed color preferences.

  17. The influence of the N(2D) and N(2P) states in the ionization of the pink afterglow of the nitrogen flowing DC discharge

    Science.gov (United States)

    Levaton, J.; Klein, A. N.; Binder, C.

    2018-01-01

    In the present work, we extensively discuss the role of N(2D) and N(2P) atoms in the ionization processes of pink afterglow based on optical emission spectroscopy analysis and kinetic numerical modelling. We studied the pink afterglow generated by a nitrogen DC discharge operating at 0.6 Slm-1 flow rate, 45 mA discharge current and pressures ranging from 250 to 1050 Pa. The 391.4 nm nitrogen band was monitored along the afterglow furnishing the relative density of the N2+(B2Σ+u, v = 0) state. A numerical model developed to calculate the nitrogen species densities in the afterglow fits the excited ion density profiles well for the experimental conditions. From the modelling results, we determine the densities of the N+, N2+, N3+, and N4+ ions; the calculations show that the N3+ ion density predominates in the afterglow at the typical residence times of the pink afterglow. This behaviour has been observed experimentally and reported in the literature. Furthermore, we calculate the fractional contribution in the ionization for several physical-chemical mechanisms in the post-discharge. Even with the N3+ ion density being dominant in the afterglow, we find through the calculations that the ionization is dominated by the reactions N(2D) + N(2P) → N2+(X2Σ+g) + e and N2(a'1Σ-u) + N2(X 1Σg+, v > 24) → N4+ + e. The ion conversion mechanisms, or ion transfer reactions, which are responsible for the fact that the N3+ density dominates in the post-discharge, are investigated.

  18. An assessment of oil-spill effects on pink salmon populations following the Exxon Valdez oil spill. Part 2: Adults and escapement

    International Nuclear Information System (INIS)

    Maki, A.W.; Brannon, E.J.; Gilbertson, L.G.; Moulton, L.L.; Skalski, J.R.

    1995-01-01

    This paper presents results of a field program designed to monitor the status of wildstock pink salmon populations in Prince William Sound following the Exxon Valdez oil spill. Field counts of spawning salmon were conducted each year from 1989 through 1992 to test for spill effects o the distribution nd abundance of pink salmon adults spawning in selected streams in the southwestern portion of Prince William Sound, including streams from the most heavily oiled areas. Counts of whole-stream and intertidal escapement density were statistically compared for 40 study streams in 1989 and for a subset of those streams in successive years. Measurements of residual hydrocarbons were made from stream-bed sediments to test for correlations with spawning behavior. Adult pink salmon in the postspill years of 1990 and 1991, progeny of the year classes considered most vulnerable to the oil spill, returned in high numbers, with the wildstock spawners exceeding their parent year returns. In 1989, adult returns reflected the relatively weak run for that year with a mean spawner density of 0.68 fish/m 2 in reference streams and 0.69 fish/m 2 in oiled streams. In 1990, mean escapement density for reference streams was 1.40 fish/m 2 and 1.55 fish/m 2 for oiled streams, indicating the strongest run of the four study years. Trends in polycyclic aromatic hydrocarbon (PAH) concentrations for the majority of oiled streams show a general decline from 1989 to background levels by 1990. 45 refs., 14 figs., 5 tabs

  19. Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.

    Science.gov (United States)

    Fernandez-Moreno, Josefina-Patricia; Tzfadia, Oren; Forment, Javier; Presa, Silvia; Rogachev, Ilana; Meir, Sagit; Orzaez, Diego; Aharoni, Aspah; Granell, Antonio

    2016-07-01

    The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source of new alleles for breeding programs. To date, all characterized pink-pigmented tomato fruit mutants appear to result from low SlMYB12 transcript levels in the fruit skin. Two new mutant lines displaying a pink fruit phenotype (pf1 and pf2) were characterized in this study. In the pf mutants, SlMYB12 transcripts accumulated to wild-type levels but exhibited the same truncation, which resulted in the absence of the essential MYB activation domain coding region. Allelism and complementation tests revealed that both pf mutants were allelic to the y locus and showed the same recessive null allele in homozygosis: Δy A set of molecular and metabolic effects, reminiscent of those observed in the Arabidopsis (Arabidopsis thaliana) myb11 myb12 myb111 triple mutant, were found in the tomato Δy mutants. To our knowledge, these have not been described previously, and our data support the idea of their being null mutants, in contrast to previously described transcriptional hypomorphic pink fruit lines. We detected a reduction in the expression of several flavonol glycosides and some associated glycosyl transferases. Transcriptome analysis further revealed that the effects of the pf mutations extended beyond the flavonoid pathway into the interface between primary and secondary metabolism. Finally, screening for Myb-binding sites in the candidate gene promoter sequences revealed that 141 of the 152 co-down-regulated genes may be direct targets of SlMYB12 regulation. © 2016 American Society of Plant Biologists. All Rights Reserved.

  20. Regulation of the hunting season as a tool for adaptive harvest management — first results for pink-footed geese Anser brachyrhynchus

    DEFF Research Database (Denmark)

    Madsen, Jesper; Clausen, Kevin; Christensen, Thomas Kjær

    2016-01-01

    Adjustment of hunting season length is often used to regulate harvest of waterbirds but the effects are disputed. We describe the first results of season length extension on the harvest of the pink-footed goose, which has been selected as the first test case of adaptive harvest management...... of waterbirds in Europe. In Denmark, the season (previously 1 September to 31 December) was extended to include January in 2014–2015 with the aim to increase the harvest and, in the longer term, reduce the population size. The total harvest in Denmark increased by 52% compared to previous years, and almost 50...

  1. Increased opercular rates of pink salmon (Oncorhynchus gorbuscha) fry after exposure to the eater-soluble fraction of Prudhoe Bay crude oil

    Energy Technology Data Exchange (ETDEWEB)

    Thomas, R E [Chico State Univ., CA; Rice, S D

    1976-01-01

    The opercular rates of pink salmon (Oncorhynchus gorbuscha) fry were measured during 24-h exposure to sublethal concentrations of the water-soluble fraction of Prudhoe Bay crude oil. Opercular rates increased significantly for as long as 9 and 12 h after exposure to water-soluble fractions prepared from oil-water solutions of 2.83 and 3.46 ppM. The increases in rates were proportional to increases in dose. Recording changes in opercular rates appears to be a suitable method for detecting sublethal physiological effects of stress, because the observed changes occurred at approximately 20 percent of the 96 h-LC50.

  2. Synthesis and structure of a pink pigment in the system Zr02-TiO2-SnO2-Cr2O3

    International Nuclear Information System (INIS)

    Zygadlo, M.

    1979-01-01

    The mechanism has been discussed of the formation of this pigment in the ceramic glazes and the results are communicated of the experiment to synthetize this pigment and to test it by the thermographic, X-ray and electron-microsonde methods. It has been found that the pink pigment is the result of dispersion of Cr 2 O 3 upon the grain areas of Zr0 2 , TiO 2 and SnO 2 : it is not a solid solution of Cr 2 O 3 in these oxides as has been earlier suggested. (author)

  3. Limisphaera ngatamarikiensis gen. nov., sp. nov., a thermophilic, pink-pigmented coccus isolated from subaqueous mud of a geothermal hotspring.

    Science.gov (United States)

    Anders, Heike; Power, Jean F; MacKenzie, Andrew D; Lagutin, Kirill; Vyssotski, Mikhail; Hanssen, Eric; Moreau, John W; Stott, Matthew B

    2015-04-01

    A novel bacterial strain, NGM72.4(T), was isolated from a hot spring in the Ngatamariki geothermal field, New Zealand. Phylogenetic analysis based on 16S rRNA gene sequences grouped it into the phylum Verrucomicrobia and class level group 3 (also known as OPB35 soil group). NGM72.4(T) stained Gram-negative, and was catalase- and oxidase-positive. Cells were small cocci, 0.5-0.8 µm in diameter, which were motile by means of single flagella. Transmission electron micrograph (TEM) imaging showed an unusual pirellulosome-like intracytoplasmic membrane. The peptidoglycan content was very small with only trace levels of diaminopimelic acid detected. No peptidoglycan structure was visible in TEM imaging. The predominant isoprenoid quinone was MK-7 (92%). The major fatty acids (>15%) were C(16 : 0), anteiso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0). Major phospholipids were phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME) and cardiolipin (CL), and a novel analogous series of phospholipids where diacylglycerol was replaced with diacylserinol (sPE, sPMME, sCL). The DNA G+C content was 65.6 mol%. Cells displayed an oxidative chemoheterotrophic metabolism. NGM72.4(T) is a strictly aerobic thermophile (growth optimum 60-65 °C), has a slightly alkaliphilic pH growth optimum (optimum pH 8.1-8.4) and has a NaCl tolerance of up to 8 g l(-1). Colonies were small, circular and pigmented pale pink. The distinct phylogenetic position and phenotypic traits of strain NGM72.4(T) distinguish it from all other described species of the phylum Verrucomicrobia and, therefore, it is considered to represent a novel species in a new genus for which we propose the name Limisphaera ngatamarikiensis gen. nov., sp. nov. The type strain is NGM72.4(T) ( = ICMP 20182(T) = DSM 27329(T)). © 2015 IUMS.

  4. Pink electrons, anyone?

    International Nuclear Information System (INIS)

    McCaughey, John.

    1997-01-01

    The deregulated electric power industry in the United States is undergoing a radical change in marketing emphasis more familiar to consumers of branded products such as soft drinks. In attempts to woo customers from other companies, utilities are beginning to market a ''company image'' offering lower prices, better products (i.e. other household services such as telephone and cable television) and improved provision levels of customer services. The price of the product, in this case electric power, has been shown to be much more likely to persuade a consumer to shift supplier than any existing level of customer satisfaction. (UK)

  5. EITSA teatab / Katrin Pink

    Index Scriptorium Estoniae

    Pink, Katrin

    2005-01-01

    Eesti Infotehnoloogia Sihtasutuse konkursist "Mobiilsustoetused - ITK erialade doktorantide mobiilsus", mille eesmärgiks on toetada Eesti avalik-õiguslike ülikoolide info- ja kommunikatsioonitehnoloogia erialade doktorantide osavõttu erialastest konverentsidest, täienduskursustest, suve- ja talvekoolidest välisriikides

  6. A Pink Writing Experiment

    Directory of Open Access Journals (Sweden)

    Teija Löytönen

    2015-10-01

    Full Text Available This article addresses a collaborative writing experiment that explores spaces of diverse encounters that began at a research conference held in the Flamingo hotel in Las Vegas; spaces where knowings emerge in the (shared moment, in-between (ourselves, prompted by different (research questions and entanglements of matter and meaning. Through these multiple and emergent writing encounters we explore ways towards collaborative scholarly writing and accessible ways of working and knowing beyond the immediately known or sensed. In addition, this collaborative writing experiment serves to inspire and engage participants (qualitative researchers and ethnographers alike to explore, share, and disseminate knowledge across contexts differently. We call for writing in qualitative research that senses, figures out, and “reveals” via moving and sensuous bodies, and emerging embodied encounters within particular spaces.

  7. Facts About Pink Eye

    Science.gov (United States)

    ... NEI Intranet (Employees Only) *PDF files require the free Adobe® Reader® software for viewing. This website is maintained by the NEI Office of Science Communications, Public Liaison, and Education. Technical questions about this website can be addressed ...

  8. The influence of EDTA application on the interactions of cadmium, zinc, and lead and their uptake of rainbow pink (Dianthus chinensis)

    International Nuclear Information System (INIS)

    Lai, H.-Y.; Chen, Z.-S.

    2006-01-01

    Soil used in this study was artificially contaminated with Cd, Zn, Pb, or applied in combinations (Cd-Zn, Cd-Pb, Zn-Pb, or Cd-Zn-Pb) to study the interactions of metals in soil contaminated with multiple metals. After planting rainbow pink (Dianthus chinensis) in these soils for 21 days, three different concentrations of ethylenedinitrilotetraacetic acid (EDTA) solutions were added to study the effect of applying EDTA on the interactions among these metals. The concentrations of Cd, Zn, and Pb in the soil solutions of different metals-treated soils increased significantly after applying 5 mmol EDTA kg -1 soil (p -1 soil. Cadmium inhibited the concentration of Zn without applying EDTA. However, whether the application of EDTA or not and the applied EDTA concentration had the greatest effect on the uptake of Pb when compared to Cd and Zn. After applying 5 mmol EDTA kg -1 soil, Cd or Zn in the Pb-contaminated soil inhibited the uptake of Pb in rainbow pink, but there were no effect in other treatments

  9. Sorafenib targets the mitochondrial electron transport chain complexes and ATP synthase to activate the PINK1-Parkin pathway and modulate cellular drug response.

    Science.gov (United States)

    Zhang, Conggang; Liu, Zeyu; Bunker, Eric; Ramirez, Adrian; Lee, Schuyler; Peng, Yinghua; Tan, Aik-Choon; Eckhardt, S Gail; Chapnick, Douglas A; Liu, Xuedong

    2017-09-08

    Sorafenib (Nexavar) is a broad-spectrum multikinase inhibitor that proves effective in treating advanced renal-cell carcinoma and liver cancer. Despite its well-characterized mechanism of action on several established cancer-related protein kinases, sorafenib causes variable responses among human tumors, although the cause for this variation is unknown. In an unbiased screening of an oncology drug library, we found that sorafenib activates recruitment of the ubiquitin E3 ligase Parkin to damaged mitochondria. We show that sorafenib inhibits the activity of both complex II/III of the electron transport chain and ATP synthase. Dual inhibition of these complexes, but not inhibition of each individual complex, stabilizes the serine-threonine protein kinase PINK1 on the mitochondrial outer membrane and activates Parkin. Unlike the protonophore carbonyl cyanide m -chlorophenylhydrazone, which activates the mitophagy response, sorafenib treatment triggers PINK1/Parkin-dependent cellular apoptosis, which is attenuated upon Bcl-2 overexpression. In summary, our results reveal a new mechanism of action for sorafenib as a mitocan and suggest that high Parkin activity levels could make tumor cells more sensitive to sorafenib's actions, providing one possible explanation why Parkin may be a tumor suppressor gene. These insights could be useful in developing new rationally designed combination therapies with sorafenib. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. p53-dependent manner of persistent activation of the radiation-induced reversion in the pink-eyed unstable mouse embryo

    International Nuclear Information System (INIS)

    Shiraishi, K.; Yonezawa, M.; Niwa, O.

    2003-01-01

    Full text: We previously reported that radiation has an ability to induce genomic instability which causes delayed and untargeted mutation. These mutations aren't accounted for by the usual relationship between DNA damages and repair. However, the mechanisms of a long-term memory of DNA damage and the persistence of up-regulated recombination activity have yet to be elucidated. The mouse pink-eyed unstable (pun) mutation is due to an intragenic duplication of the pink-eyed dilution locus and frequently reverts black to the wild type in germ cells as well as somatic cells. The frequency of reversion was estimated by counting cluster of pigment cells in retinal pigment epithelium. Twice increase of the reversion was observed in F1 mice born to 6Gy irradiated male at spermatozoa stage, but not at other spermatogenesis stages( -tid, -cyte, -gonia ). Trans-genarational effect in F2 mice also didn't observe. Therefore, this phenomenon only occurs under the restricted germ cell stage. Additionally, the reversion frequency of p53 deficient F1 mouse born to irradiated sperm was less than irradiated wild mouse. 5aza-dc chemical agent, which is DNA methylation emzyme inhibitor, also suppressed pun allele recombination in mouse embryo. These data indicate that p53 contributes delayed and untargeted mutation, perhaps, by regulation of DNA metylation status

  11. Popular o non popular? I tardi anni Sessanta, la controcultura e l’avanguardia nella musica rock. L’esempio di "Ummagumma" dei Pink Floyd

    Directory of Open Access Journals (Sweden)

    Philippe Gonin

    2017-05-01

    Full Text Available In alcuni generi che emergono nella popular music della fine degli anni Sessanta, quali progressive, art o psychedelic rock, la tensione verso la sperimentazione sembra essere la principale preoccupazione dell’ “avanguardia pop”, sia negli Stati Uniti (con figure come Frank Zappa, The Velvet Underground, The Grateful Dead, che nel Regno Unito (AMM, Pink Floyd, The Beatles, Soft Machine. In questi repertori si evidenziano due principali tipologie di approcci, che possono essere definiti “tentazione sinfonica” e “tentazione sperimentale”. L’obiettivo di questo contributo è mostrare come, alla fine degli anni Sessanta, i confini tra popular music, free jazz, musica sperimentale e “musica d’arte” si siano progressivamente indeboliti, se non addirittura siano completamente superati. A partire da una panoramica generale del decennio, il saggio si concentra sulla combinazione di modalità compositive tipiche dell’avanguardia, della musica d’arte e della popular music, concentrando l’attenzione su tre aspetti: sperimentazione nel rock della fine degli anni Sessanta; sperimentazione nella musica dei Pink Floyd; analisi di alcuni pezzi tratti dall’album Ummagumma.

  12. Knowledge regarding fertility preservation in cancer patients: a population-based survey among Brazilian people during the Pink October awareness event.

    Science.gov (United States)

    Chehin, Mauricio B; Bonetti, Tatiana Cs; Serafini, Paulo C; Motta, Eduardo LA

    2017-06-01

    The aim of this study was to assess the knowledge about the risk of infertility in cancer patients after treatment, and the options for fertility preservation based on a survey carried out during the 2013 Pink October campaign. This survey was carried out during the 2013 Pink October event in the most important public park of São Paulo, Brazil. Approximately 900 people expressed interest in learning about breast cancer prevention and fertility preservation by participating in workshops, and 242 people filled out a questionnaire. Most of the respondents (78.5%) were women, and one-fourth (25%) had at least one relative with gynecological cancer. Among women over 40 years of age, 86.3% had been screened for breast cancer at some point. However, few participants (34.0%) were aware that cancer treatment can lead to infertility or had heard about fertility preservation options (22.0%). Having a relative with cancer did not influence their knowledge about fertility preservation (22.4% versus 21.3%; p=0.864). However, a higher educational level was significantly associated with more knowledge about the effects of cancer on fertility and options for fertility preservation. The majority of participants did not have knowledge about the impact of oncologic treatment on fertility and did not know that there are options to preserve fertility in cancer patients. Awareness of infertility risk factors is an essential first step to safeguard future fertility, and therefore, more educational initiatives are needed to spread knowledge about oncofertility.

  13. Seasonal marine growth of Bristol Bay sockeye salmon (Oncorhynchus nerka) in relation to competition with Asian pink salmon (O. gorbuscho) and the 1977 ocean regime shift

    Science.gov (United States)

    Ruggerone, Gregory T.; Farley, Ed; Nielsen, Jennifer L.; Hagen, Peter

    2005-01-01

    Recent research demonstrated significantly lower growth and survival of Bristol Bay sockeye salmon (Oncorhynchus nerka) during odd-numbered years of their second or third years at sea (1975, 1977, etc.), a trend that was opposite that of Asian pink salmon (O. gorbuscha) abundance. Here we evaluated seasonal growth trends of Kvichak and Egegik river sockeye salmon (Bristol Bay stocks) during even- and odd-numbered years at sea by measuring scale circuli increments within each growth zone of each major salmon age group between 1955 and 2000. First year scale growth was not significantly different between odd- and even-numbered years, but peak growth of age-2. smolts was significantly higher than age-1 smolts. Total second and third year scale growth of salmon was significantly lower during odd- than during even-numbered years. However, reduced scale growth in odd-numbered years began after peak growth in spring and continued through summer and fall even though most pink salmon had left the high seas by late July (10-18% growth reduction in odd vs. even years). The alternating odd and even year growth pattern was consistent before and after the 1977 ocean regime shift. During 1977-2000, when salmon abundance was relatively great, sockeye salmon growth was high during specific seasons compared with that during 1955-1976, that is to say, immediately after entry to Bristol Bay, after peak growth in the first year, during the middle of the second growing season, and during spring of the third season. Growth after the spring peak in the third year at sea was relatively low during 1977-2000. We hypothesize that high consumption rates of prey by pink salmon during spring through mid-July of odd-numbered years, coupled with declining zooplankton biomass during summer and potentially cyclic abundances of squid and other prey, contributed to reduced prey availability and therefore reduced growth of Bristol Bay sockeye salmon during late spring through fall of odd-numbered years.

  14. The effect of silicon foliar application on the development of Season ornamental plants. Part II: Argyranthemum frutescens 'Blazer Rose', Xerochrysum bracteatum 'Gold', Osteospermum ecklonis 'Grande Pink Blush' and Gaura lindheimeri 'Corinas Choice'

    OpenAIRE

    Katarzyna Wróblewska; Regina Dębicz

    2012-01-01

    Recent research has proved that supplementary application of silicon, involved in plant tolerance against many stress factors, positively influences plant development improving the quality of ornamental plants. A two-factorial experiment involved four cultivars of ornamental plants: Argyranthemum frutescens 'Blazer Rose', Xerochrysum bracteatum 'Gold', Osteospermum ecklonis 'Grande Pink Blush', and Gaura lindheimeri 'Corinas Choice' The first experimental factor was the concentration of Actis...

  15. Ecology of planktonic foraminifera and their symbiotic algae

    International Nuclear Information System (INIS)

    Gastrich, M.D.

    1986-01-01

    Two types of symbiotic algae occurred abundantly and persistently in the cytoplasm of several species of planktonic Foraminifera over a ten year period in different tropical and subtropical areas of the North Atlantic Ocean. These planktonic Foraminifera host species consistently harbored either dinoflagellates or a newly described minute coccoid algal type. There appeared to be a specific host-symbiont relationship in these species regardless of year, season or geographic locality. The larger ovoid dinoflagellates (Pyrrhophycophyta) occur in the spinose species Globigerinoides ruber, Globigerinoides sacculifer, G. conglobatus and Orbulina universa. The smaller alga, from 1.5 to 3.5 um in diameter, occurs in one spinose species Globigerinella aequilateralis and also in the non-spinose species Globigerinita glutinata, Globoquadrina dutertrei, Globorotalia menardii, Globorotalia cristata, Globorotalia inflata, Candeina nitida, in various juvenile specimens and at all seasons except the winter months in Pulleniatina obliquiloculata and Globorotalial hirsuta. Controlled laboratory studies indicated a significant C incorporation into the host cytoplasm and inorganic calcium carbonate test of Globigerinoides ruber. During incubation for up to two hours, the 14 C uptake into the cytoplasm and test in the light was significantly greater than uptake in the dark by living specimens or by dead foraminifers. There appears to be light-enhanced uptake of 14 C into the test with dinoflagellate photosynthesis contributing to host calcification. In culture, symbiotic algae were observed to survive for the duration of the lifespan of their hosts

  16. Immediate Restoration of Immediate Implants in the Esthetic Zone of the Maxilla Via the Copy-Abutment Technique: 5-Year Follow-Up of Pink Esthetic Scores.

    Science.gov (United States)

    Fürhauser, Rudolf; Mailath-Pokorny, Georg; Haas, Robert; Busenlechner, Dieter; Watzek, Georg; Pommer, Bernhard

    2017-02-01

    Implant esthetics may benefit from individualized zirconia abutments copying the emergence profile of the natural tooth and delivered within days after immediate implant insertion. To investigate the esthetic outcome of the Copy-Abutment technique using the Pink Esthetic Score (PES). A total of 77 patients with single-tooth implants in the anterior maxilla restored at the day of immediate implant placement using Copy-Abutments and provisional crowns were followed-up after 1 week, 1 month, 4 months, 6 months, 1, 2, 3, 4, and 5 years to assess implant esthetics. PES ranged between 7 and 14 (median: 13) and improved significantly between the 6 month and 1 year follow-up (p esthetic zone show satisfactory long-term esthetic outcomes. © 2016 Wiley Periodicals, Inc.

  17. On the difficulty of N({sup 4}S) atom recombination to explain the appearance of the pink afterglow in a N{sub 2} flowing discharge

    Energy Technology Data Exchange (ETDEWEB)

    Loureiro, J [Centro de Fisica dos Plasmas, Instituto Superior Tecnico, 1049-001 Lisbon (Portugal); Sa, P A [Centro de Fisica dos Plasmas, Instituto Superior Tecnico, 1049-001 Lisbon (Portugal); Departamento de Fisica, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto (Portugal); Guerra, V [Centro de Fisica dos Plasmas, Instituto Superior Tecnico, 1049-001 Lisbon (Portugal)

    2006-01-07

    The possibility that the pink afterglow (PA) of a flowing nitrogen discharge occurs as a result of recombination of N({sup 4}S) atoms is evaluated and discussed, based on a detailed kinetic model for a microwave discharge and post-discharge. The present simulation shows that the N{sub 2}{sup +}(B{sup 2}{sigma}{sub u}{sup +},v) states responsible for the emission of the PA cannot be created via an indirect mechanism initiated with atomic recombination. Alternatively, it is indicated that the PA may have its origin in non-resonant vibration-vibration energy-exchange processes between N{sub 2}(X{sup 1}{sigma}{sub g}{sup +},v) molecules, which lead to an overpopulation of high levels of the vibrational manifold.

  18. On the difficulty of N(4S) atom recombination to explain the appearance of the pink afterglow in a N2 flowing discharge

    International Nuclear Information System (INIS)

    Loureiro, J; Sa, P A; Guerra, V

    2006-01-01

    The possibility that the pink afterglow (PA) of a flowing nitrogen discharge occurs as a result of recombination of N( 4 S) atoms is evaluated and discussed, based on a detailed kinetic model for a microwave discharge and post-discharge. The present simulation shows that the N 2 + (B 2 Σ u + ,v) states responsible for the emission of the PA cannot be created via an indirect mechanism initiated with atomic recombination. Alternatively, it is indicated that the PA may have its origin in non-resonant vibration-vibration energy-exchange processes between N 2 (X 1 Σ g + ,v) molecules, which lead to an overpopulation of high levels of the vibrational manifold

  19. Diurnal variation in the behaviour of the Pink-footed Goose (Anser brachyrhynchus) during the spring stopover in Trøndelag, Norway

    DEFF Research Database (Denmark)

    Chudzinska, Magda Ewa; Madsen, Jesper; Nabe-Nielsen, Jacob

    2013-01-01

    behaviour at a staging site and assess the extent to which behavioural patterns are attributable to physiological factors (digestibility of the food) and environmental conditions (flock size, type and frequency of disturbance and distance to roost). We found that feeding activity peaked at mid-day, whereas...... different energetic and nutrient demands when at spring staging sites. Seasonal changes in habitat availability as well as density dependence may also affect the birds’ behavioural patterns. A sporadic, unpredictable disturbance reduced the proportion of geese feeding to a greater extent than a predictable...... the birds were most alert in the morning and afternoon. The behaviour of Pink-footed Goose also varied with habitat type, disturbance level and distance to roost. The diurnal variation in feeding activity differed from behaviour reported for geese on the wintering grounds, indicating that the birds have...

  20. Implementation of the first adaptive management plan for a European migratory waterbird population: The case of the Svalbard pink-footed goose Anser brachyrhynchus

    Science.gov (United States)

    Madsen, Jesper; Williams, James Henty; Johnson, Fred A.; Tombre, Ingunn M.; Dereliev, Sergey; Kuijken, Eckhart

    2017-01-01

    An International Species Management Plan for the Svalbard population of the pink-footed goose was adopted under the Agreement on the Conservation of African-Eurasian Migratory Waterbirds in 2012, the first case of adaptive management of a migratory waterbird population in Europe. An international working group (including statutory agencies, NGO representatives and experts) agreed on objectives and actions to maintain the population in favourable conservation status, while accounting for biodiversity, economic and recreational interests. Agreements include setting a population target to reduce agricultural conflicts and avoid tundra degradation, and using hunting in some range states to maintain stable population size. As part of the adaptive management procedures, adjustment to harvest is made annually subject to population status. This has required streamlining of monitoring and assessment activities. Three years after implementation, indicators suggest the attainment of management results. Dialogue, consensus-building and engagement among stakeholders represent the major process achievements.

  1. Comparison of X-ray and gamma-ray dose-response curves for pink somatic mutations in Tradescantia clone 02

    International Nuclear Information System (INIS)

    Underbrink, A.G.; Kellerer, A.M.; Mills, R.E.; Sparrow, A.H.; Brookhaven National Lab., Upton, N.Y.

    1976-01-01

    Microdosimetric data indicate that the mean specific energy, xi, produced by individual charged particles from X rays and gamma rays is different for the two radiation qualities by nearly a factor of two. In order to test whether this influences the initial, linear component in the dose-effect relations, a comparison was made between dose-response curves for pink somatic mutations in Tradescantia clone 02 stamen hairs following X and gamma irradiations. Absorbed doses ranged from 2.66 to 300 rad. The results are in agreement with predictions made on the basis of microdosimetric data. At low doses gamma rays are substantially less effective than X rays. The RBE of gamma rays vs. X rays at low doses was approximately 0.6, a value lower than those usually reported in other experimental systems. (orig.) [de

  2. Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides x nigra DN34).

    Science.gov (United States)

    Van Aken, Benoit; Peres, Caroline M; Doty, Sharon Lafferty; Yoon, Jong Moon; Schnoor, Jerald L

    2004-07-01

    A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001T, was isolated from internal poplar tissues (Populus deltoidesxnigra DN34) and identified as a member of the genus Methylobacterium. Phylogenetic analyses showed that strain BJ001T is related to Methylobacterium thiocyanatum, Methylobacterium extorquens, Methylobacterium zatmanii and Methylobacterium rhodesianum. However, strain BJ001T differed from these species in its carbon-source utilization pattern, particularly its use of methane as the sole source of carbon and energy, an ability that is shared with only one other member of the genus, Methylobacterium organophilum. In addition, strain BJ001T is the only member of the genus Methylobacterium to be described as an endophyte of poplar trees. On the basis of its physiological, genotypic and ecological properties, the isolate is proposed as a member of a novel species of the genus Methylobacterium, Methylobacterium populi sp. nov. (type strain, BJ001T=ATCC BAA-705T=NCIMB 13946T).

  3. The preventative power of "pink": Delineating the effects of social identification and protection motivation theory in breast cancer-related advertisements.

    Science.gov (United States)

    Devlin, Michael B; Dillard, Sydney

    2016-01-01

    An online experiment was conducted to assess the extent gender-salient breast cancer awareness advertisements had on influencing risk perception, encouraging preventative behaviors, and gathering health information. Social identification theory and protection motivation theory postulate gender-salient pink-branded advertisements trigger defense mechanisms, countering desired outcomes. This study concludes gender-salient ads focusing on women, and displaying the Susan G. Komen logo caused aversive behaviors, whereas gender-neutral ads, showing medical providers and logos such as American Medical Association, improve health-related reporting. Results also highlight a disconnect between women who socially identify as breast cancer survivors/supporters, and those with no prior experience with breast cancer.

  4. Characterization of the key aroma compounds in pink guava (Psidium guajava L.) by means of aroma re-engineering experiments and omission tests.

    Science.gov (United States)

    Steinhaus, Martin; Sinuco, Diana; Polster, Johannes; Osorio, Coralia; Schieberle, Peter

    2009-04-08

    Seventeen aroma-active volatiles, previously identified with high flavor dilution factors in fresh, pink Colombian guavas (Psidium guajava L.), were quantified by stable isotope dilution assays. On the basis of the quantitative data and odor thresholds in water, odor activity values (OAV; ratio of concentration to odor threshold) were calculated. High OAVs were determined for the green, grassy smelling (Z)-3-hexenal and the grapefruit-like smelling 3-sulfanyl-1-hexanol followed by 3-sulfanylhexyl acetate (black currant-like), hexanal (green, grassy), ethyl butanoate (fruity), acetaldehyde (fresh, pungent), trans-4,5-epoxy-(E)-2-decenal (metallic), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (caramel, sweet), cinnamyl alcohol (floral), methyl (2S,3S)-2-hydroxy-3-methylpentanoate (fruity), cinnamyl acetate (floral), methional (cooked potato-like), and 3-hydroxy-4,5-dimethyl-2(5H)-furanone (seasoning-like). Studies on the time course of odorant formation in guava puree or cubes, respectively, showed that (Z)-3-hexenal was hardly present in the intact fruits, but was formed very quickly during crushing. The aroma of fresh guava fruit cubes, which showed a very balanced aroma profile, was successfully mimicked in a reconstitute consisting of 13 odorants in their naturally occurring concentrations. Omission tests, in which single odorants were omitted from the entire aroma reconstitute, revealed (Z)-3-hexenal, 3-sulfanyl-1-hexanol, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, 3-sulfanylhexyl acetate, hexanal, ethyl butanoate, cinnamyl acetate, and methional as the key aroma compounds of pink guavas.

  5. Some new and noteworthy diseases of poplars in India. [Botryodiplodia sett-rot; Alternaria tip blight; Cladosporium leaf spot; Fusarium pink incrustation

    Energy Technology Data Exchange (ETDEWEB)

    Singh, S.

    1983-09-01

    Four new diseases of poplars namely Botryodiplodia sett-rott, Alternaria tip blight, Cladosporium leaf spot and Fusarium pink incrustation are described in this paper. Botryodiplodia palmarum causes sett-rott of poplars both at pre-sprouting and post-sprouting stage. The pathogen also causes mortality of poplar plants in the field within 4-6 weeks after planting. Alternaria stage of Pleuspora infectoria has been found as the cause of blackening and dying of growing tips and young leaves of a Populus sp. and P. deltoides in nurseries. Cladosporium humile has been recorded as the cause of brown spot followed by crumpling and premature shedding of leaves in P. ciliata, P. nigra and P. alba. The cause of Fusarium incrustation disease on P. cilata has been identified as Fusarium sp. of Gibbosum group. Pathogenicity of Botryodiplodia palmarum and Alternaria stage of Pleospora infectoria was confirmed by artificial inoculations. Brief descriptions of Alternaria, Cladosporium and Fusarium are also given. The paper also gives a short account of some noteworthy diseases recorded on poplars namely Ganoderma root rot, foliage ruts and stem cankers. Ganoderma root-rot is found to reach alarming proportions in closely spaced poplar plantations. Melampsora ciliata, an indigenous rust, is found to attack mainly clones of P. deltoides, P. yunnanensis, P. trichocarpa, P. alba and some cultivars of P. x euramericana in nurseries. A brief account of three types of stem cankers i.e. cankers due to pink disease fungus, Corticium salmonicolor, sun-scaled cankers and cankers associated with slime flux on various clones of P. deltoides is also given.

  6. Paleomonsoon records from the western Arabian Sea: evidence of aeolian input and Red Sea water inflow

    International Nuclear Information System (INIS)

    Tiwari, M.; Ramesh, R.; Bhushan, R.; Somayajulu, B.L.K.; Jull, A.J.T.; Burr, G.S.; Sheshshayee, M.S.

    2004-01-01

    The western Arabian Sea experiences intense productivity variations due to wind-induced upwelling during the South West monsoon. Variations in calcareous productivity derived from marine sediment cores can prove to be an excellent indicator of monsoon wind strength provided that the core has been raised from water depths well above the lysocline (∼4000 m). The core was radiocarbon dated by on select planktonic foraminiferal species viz. Globigerinoides ruber, Globigerinoides sacculifer, Orbulina universa and Neogloboquadrina dutertrei (size range chosen for dating as well as isotopic analysis is 250 μ -500 μ. Planktonic foraminifera were selected for our study because they inhabit the surface and near surface oceans (upto∼ 100 m) and, therefore, readily incorporate changes occurring in the surface ocean into their calcitic shells

  7. Characterization of phenolic and other polar compounds in peel and flesh of pink guava (Psidium guajava L. cv. 'Criolla') by ultra-high performance liquid chromatography with diode array and mass spectrometric detection.

    Science.gov (United States)

    Rojas-Garbanzo, Carolina; Zimmermann, Benno F; Schulze-Kaysers, Nadine; Schieber, Andreas

    2017-10-01

    Pink guava (Psidium guajava L.) is a highly consumed fruit in tropical countries. Despite of interesting research on health effects of this fruit, investigations into the profile of secondary plant metabolites are scarce. In this study, the phenolic compounds in the peel and flesh of pink guava were characterized by ultra-high performance liquid chromatography with diode array and mass spectrometric detection. Sixty phenolic compounds were characterized by MS 2 and classified as ellagitannins, flavones, flavonols, flavanols, proanthocyanidins, dihydrochalcones, and anthocyanidins, and non-flavonoids such as phenolic acid derivatives, stilbenes, acetophenones, and benzophenones. Forty-two polyphenols are reported for the first time in both peel and flesh, and twenty-four compounds were detected for the first time in P. guajava, e.g., phlorizin, nothofagin, astringin, chrysin-C-glucoside, valoneic acid bilactone, cinnamoyl-glucoside, and two dimethoxycinnamoyl-hexosides. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Survival of pink salmon (Oncorhynchus gorbuscha) fry to adulthood following a 10-day exposure to aromatic hydrocarbon water-soluble fraction of crude oil and release to the Pacific Ocean

    International Nuclear Information System (INIS)

    Birtwell, I. K.; Fink, R.; Brand, D.; Alexander, R.; McAllister, C. D.

    1999-01-01

    Potential effects of hydrocarbons on salmonids were investigated. In the course of the experiment pink salmon fry were exposed for 10 days to seawater containing varying controlled doses of the water-soluble fractions of North Slope crude oil, comprised primarily of monoaromatics. After exposure the fry were released into the Pacific Ocean to complete their life cycle. The experiments were replicated for three years following the original experiment. No consistent dose-dependent effect of the 10-day exposure on the growth and survival to maturity of the pink salmon was observed, although there was high mortality following each initial release. These results may be considered as one measure of resistance to exposure to contaminants, however, the method is not recommended for general use because it is based on a highly selected sample (i.e. those that survived the initial exposure), and the same results may not apply to populations that have not been subjected to intensive nonselective fishing pressures. In summary, the survival of some fraction of the population of pink salmon is evidence of the robustness of some of these fish, but it is at best a crude indicator of environmental health. 44 refs., 2 tabs., 2 figs

  9. Loss of VPS13C Function in Autosomal-Recessive Parkinsonism Causes Mitochondrial Dysfunction and Increases PINK1/Parkin-Dependent Mitophagy.

    Science.gov (United States)

    Lesage, Suzanne; Drouet, Valérie; Majounie, Elisa; Deramecourt, Vincent; Jacoupy, Maxime; Nicolas, Aude; Cormier-Dequaire, Florence; Hassoun, Sidi Mohamed; Pujol, Claire; Ciura, Sorana; Erpapazoglou, Zoi; Usenko, Tatiana; Maurage, Claude-Alain; Sahbatou, Mourad; Liebau, Stefan; Ding, Jinhui; Bilgic, Basar; Emre, Murat; Erginel-Unaltuna, Nihan; Guven, Gamze; Tison, François; Tranchant, Christine; Vidailhet, Marie; Corvol, Jean-Christophe; Krack, Paul; Leutenegger, Anne-Louise; Nalls, Michael A; Hernandez, Dena G; Heutink, Peter; Gibbs, J Raphael; Hardy, John; Wood, Nicholas W; Gasser, Thomas; Durr, Alexandra; Deleuze, Jean-François; Tazir, Meriem; Destée, Alain; Lohmann, Ebba; Kabashi, Edor; Singleton, Andrew; Corti, Olga; Brice, Alexis

    2016-03-03

    Autosomal-recessive early-onset parkinsonism is clinically and genetically heterogeneous. The genetic causes of approximately 50% of autosomal-recessive early-onset forms of Parkinson disease (PD) remain to be elucidated. Homozygozity mapping and exome sequencing in 62 isolated individuals with early-onset parkinsonism and confirmed consanguinity followed by data mining in the exomes of 1,348 PD-affected individuals identified, in three isolated subjects, homozygous or compound heterozygous truncating mutations in vacuolar protein sorting 13C (VPS13C). VPS13C mutations are associated with a distinct form of early-onset parkinsonism characterized by rapid and severe disease progression and early cognitive decline; the pathological features were striking and reminiscent of diffuse Lewy body disease. In cell models, VPS13C partly localized to the outer membrane of mitochondria. Silencing of VPS13C was associated with lower mitochondrial membrane potential, mitochondrial fragmentation, increased respiration rates, exacerbated PINK1/Parkin-dependent mitophagy, and transcriptional upregulation of PARK2 in response to mitochondrial damage. This work suggests that loss of function of VPS13C is a cause of autosomal-recessive early-onset parkinsonism with a distinctive phenotype of rapid and severe progression. Copyright © 2016 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

  10. Esthetics of Flapless Single-Tooth Implants in the Anterior Maxilla Using Guided Surgery: Association of Three-Dimensional Accuracy and Pink Esthetic Score.

    Science.gov (United States)

    Fürhauser, Rudolf; Mailath-Pokorny, Georg; Haas, Robert; Busenlechner, Dieter; Watzek, Georg; Pommer, Bernhard

    2015-10-01

    Flapless implant placement using surgical templates may guarantee predictable and esthetic results provided that preplanned implant positions are transfered precisely into surgical reality. The study aims to investigate the effect of three-dimensional accuracy in guided implant surgery on the esthetic outcome of single-tooth implants in the anterior maxilla. Single-tooth implants for delayed replacement of upper incisors were inserted in 27 patients using stereolithographic templates. Superimposition of postoperative cone beam computed tomography (CBCT) scans allowed assessment of positional inaccuracy at the implant shoulder and apex, as well as angular deviation. Objective evaluation of implant esthetics was performed after a mean follow-up of 2.3 years using the Pink Esthetic Score (PES). Mean deviation between planned and actual implant position was 0.84 mm at the implant shoulder and significantly correlated to average PES of 12 (p = .031). Inaccuracy toward the buccal side was most frequent (70%). Deviations  ≥  0.8  mm resulted in significantly worse implant esthetics (median PES: 9.5, interquartile range [IQR]: 8-11) compared with more accurate implant positions (median PES: 13, IQR: 12-13, p = .039). Positional inaccuracy is low in guided implant surgery, but may however significantly compromise implant esthetics in the anterior maxilla. © 2014 Wiley Periodicals, Inc.

  11. Feasibility of integrating radiation-induced F1 sterility and biological control for population suppression of the pink bollworm, Pectinophora gossypiella, in Pakistan

    International Nuclear Information System (INIS)

    Ahmad, N.; Ashraf, M.; Hussain, T.; Qureshi, Z.A.

    2002-01-01

    Substitution of casein and wheat germ with locally available ingredients (chickpea flour, soybean flour, wheat husk and sawdust) in the specified casein-wheat germ diet affected various biological parameters of pink bollworm (PBW), Pectinophora gossypiella. The diet containing chickpea flour performed significantly better and is more economical than the other diets tested. The highest PBW field populations were recorded in the month of October when large numbers of fruiting bodies were present in the cotton. Field behavioural observations revealed that mating and other sexual activities of treated and native moths varied significantly with time of night and peak activity was during 03:00-04:00 hours. Male moths treated with 100 Gy as mature pupae responded well to gossyplure baited traps. The attraction of male moths to irradiated virgin females decreased significantly with increasing doses of radiation. Male moths responded more readily to virgin untreated females than to irradiated females. Field-cage studies demonstrated that irradiated moths (100 Gy) released at a 50:1 treated to normal ratio at three week intervals reduced larval infestations inside the cages to subeconomic level. Studies suggested that there is a great potential for integrating the egg parasitoid, Trichogramma chilonis, with the sterile insect technique to control cotton bollworms. (author)

  12. Population genetic structure of cotton pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) using mitochondrial cytochrome oxidase I (COI) gene sequences from India.

    Science.gov (United States)

    Sridhar, J; Chinna Babu Naik, V; Ghodke, A; Kranthi, S; Kranthi, K R; Singh, B P; Choudhary, J S; Krishna, M S R

    2017-11-01

    Pink bollworm (PBW), Pectinophora gossypiella is one of the most destructive pest's globally inflicting huge economic losses in cotton even during later stages of crop growth. In the present investigation, the population genetic structure, distribution, and genetic diversity of P. gossypiella in cotton growing zones of India using partial mitochondrial DNA cytochrome oxidase-I (COI) gene was addressed. The overall haplotype (Hd), number of nucleotide differences (K), and nucleotide diversity (π) were 0.3028, 0.327, and 0.00047, respectively which suggest that entire population exhibited low level of genetic diversity. Zone-wise clustering of population revealed that central zone recorded low level of Hd (0.2730) as compared to north (0.3619) and south (0.3028) zones. The most common haplotype (H1) reported in all 19 locations could be proposed as ancestral/original haplotype. This haplotype with one mutational step formed star-like phylogeny connected with 11 other haplotypes. The phylogenetic relationship studies revealed that most haplotypes of populations are closely related to each other. Haplotype 5 was exclusively present in Dharwad (South zone) shared with populations of Hanumangarh and Bathinda (North zone). The result indicated that there is no isolation by distance effect among the Indian populations of PBW. The present study reports a low genetic diversity among PBW populations of India and H1, as ancestral haplotype from which other haplotypes have evolved suggests that the migration and dispersal over long distance and invasiveness are major factors.

  13. Habitat selection of the pink shrimp Farfantepenaeus paulensis and the blue crab Callinectes sapidus in an estuary in southern Brazil: influence of salinity and submerged seagrass meadows

    Directory of Open Access Journals (Sweden)

    Vinicius Mendes Ruas

    Full Text Available This study was conducted in two estuarine inlets (Saco da Mangueira and Saco do Arraial at the Patos Lagoon estuary, southern Brazil. The changes in relative abundance and size of post-larvae and juvenile shrimp Farfantepenaeus paulensis and juvenile blue crab Callinectes sapidus were compared, considering the influence of salinity and the presence of submerged seagrass meadows. The analyses were performed using generalized linear models (GLM for abundance variations and ANOVA for variations on the size of individuals. The pink shrimp was more abundant at Saco da Mangueira, in seagrass meadows and areas of higher salinity. The blue crab was more abundant at Saco do Arraial and in lower levels of salinity. The importance of submerged vegetation for the blue crab lies in a preference of smaller crabs of the species for the seagrass meadows. It has been shown that these species choose different habitats in the estuary, and both the salinity and the presence of submerged seagrass meadows influence the selection of habitat.

  14. Distribution pattern of juveniles of the pink shrimps Farfantepenaeus brasiliensis (Latreille, 1817 and F. paulensis (Pérez-Farfante, 1967 on the southeastern Brazilian coast

    Directory of Open Access Journals (Sweden)

    Rogério Caetano da Costa

    Full Text Available Abstract The spatio-temporal distribution of juveniles of the pink shrimps Farfantepenaeus brasiliensis (Latreille, 1817 and Farfantepenaeus paulensis (Pérez-Farfante, 1967 in the Ubatuba region (SP was investigated. Sampling was performed in the bays of Ubatumirim (UBM, Ubatuba (UBA and Mar Virado (MV. A total of 2,018 F. brasiliensis and F. paulensis were collected. The largest catch of juveniles of both species occurred in UBA (N = 867, followed by UBM (N = 729 and MV (N= 422. The bottom sediment in MV had the highest silt and clay content, which explains the negative correlation of the substrate with the abundance of both species. Temperature was positively correlated with the abundance of both species. Juveniles were highly abundant in shallower areas in the summer of 1998. The high rainfall in this El Niño period may have lowered the salinity in estuarine waters and led the shrimps to move to coastal areas in search of higher salinities such as in bays. With this unusually early reduction in salinity, individuals migrated to the bay before the closed season began and thus became more exposed to fishing. We confirmed that monitoring environmental variations, especially in El Niño years, is essential for understanding the distribution patterns of juveniles of both species.

  15. Microbial dynamics during shelf-life of industrial Ricotta cheese and identification of a Bacillus strain as a cause of a pink discolouration.

    Science.gov (United States)

    Sattin, E; Andreani, N A; Carraro, L; Fasolato, L; Balzan, S; Novelli, E; Squartini, A; Telatin, A; Simionati, B; Cardazzo, B

    2016-08-01

    Dairy products are perishable and have to be preserved from spoilage during the food chain to achieve the desired shelf-life. Ricotta is a typical Italian soft dairy food produced by heat coagulation of whey proteins and is considered to be a light and healthy product. The shelf-life of Ricotta could be extended, as required by the international food trade market; however, heat resistant microflora causes spoilage and poses issues regarding the safety of the product. Next-generation sequencing (NGS) applied to the Ricotta samples defined the composition of the microbial community in-depth during the shelf-life. The analysis demonstrated the predominance of spore-forming bacteria throughout the shelf-life, mostly belonging to Bacillus, Paenibacillus and Clostridium genera. A strain involved in spoilage and causing a pink discolouration of Ricotta was isolated and characterised as Bacillus mycoides/weihenstephanensis. This is the first report of a food discolouration caused by a toxigenic strain belonging to the Bacillus cereus group that resulted the predominant strain in the community of the defective ricotta. These results suggest that the processing of raw materials to eliminate spores and residual microflora could be essential for improving the quality and the safety of the product and to extend the shelf-life of industrial Ricotta. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. The abundance of pink-pigmented facultative methylotrophs in the root zone of plant species in invaded coastal sage scrub habitat.

    Science.gov (United States)

    Irvine, Irina C; Brigham, Christy A; Suding, Katharine N; Martiny, Jennifer B H

    2012-01-01

    Pink-pigmented facultative methylotrophic bacteria (PPFMs) are associated with the roots, leaves and seeds of most terrestrial plants and utilize volatile C(1) compounds such as methanol generated by growing plants during cell division. PPFMs have been well studied in agricultural systems due to their importance in crop seed germination, yield, pathogen resistance and drought stress tolerance. In contrast, little is known about the PPFM abundance and diversity in natural ecosystems, let alone their interactions with non-crop species. Here we surveyed PPFM abundance in the root zone soil of 5 native and 5 invasive plant species along ten invasion gradients in Southern California coastal sage scrub habitat. PPFMs were present in every soil sample and ranged in abundance from 10(2) to 10(5) CFU/g dry soil. This abundance varied significantly among plant species. PPFM abundance was 50% higher in the root zones of annual or biennial species (many invasives) than perennial species (all natives). Further, PPFM abundance appears to be influenced by the plant community beyond the root zone; pure stands of either native or invasive species had 50% more PPFMs than mixed species stands. In sum, PPFM abundance in the root zone of coastal sage scrub plants is influenced by both the immediate and surrounding plant communities. The results also suggest that PPFMs are a good target for future work on plant-microorganism feedbacks in natural ecosystems.

  17. Characterization of the aroma-active compounds in pink guava (Psidium guajava, L.) by application of the aroma extract dilution analysis.

    Science.gov (United States)

    Steinhaus, Martin; Sinuco, Diana; Polster, Johannes; Osorio, Coralia; Schieberle, Peter

    2008-06-11

    The volatiles present in fresh, pink-fleshed Colombian guavas ( Psidium guajava, L.), variety regional rojo, were carefully isolated by solvent extraction followed by solvent-assisted flavor evaporation, and the aroma-active areas in the gas chromatogram were screened by application of the aroma extract dilution analysis. The results of the identification experiments in combination with the FD factors revealed 4-methoxy-2,5-dimethyl-3(2 H)-furanone, 4-hydroxy-2,5-dimethyl-3(2 H)-furanone, 3-sulfanylhexyl acetate, and 3-sulfanyl-1-hexanol followed by 3-hydroxy-4,5-dimethyl-2(5 H)-furanone, ( Z)-3-hexenal, trans-4,5-epoxy-( E)-2-decenal, cinnamyl alcohol, ethyl butanoate, hexanal, methional, and cinnamyl acetate as important aroma contributors. Enantioselective gas chromatography revealed an enantiomeric distribution close to the racemate in 3-sulfanylhexyl acetate as well as in 3-sulfanyl-1-hexanol. In addition, two fruity smelling diastereomeric methyl 2-hydroxy-3-methylpentanoates were identified as the ( R,S)- and the ( S,S)-isomers, whereas the ( S,R)- and ( R,R)-isomers were absent. Seven odorants were identified for the first time in guavas, among them 3-sulfanylhexyl acetate, 3-sulfanyl-1-hexanol, 3-hydroxy-4,5-dimethyl-2(5 H)-furanone, trans-4,5-epoxy-( E)-2-decenal, and methional were the most odor-active.

  18. Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice.

    Science.gov (United States)

    Madhaiyan, Munusamy; Kim, Byung-Yong; Poonguzhali, Selvaraj; Kwon, Soon-Wo; Song, Myung-Hee; Ryu, Jeoung-Hyun; Go, Seung-Joo; Koo, Bon-Sung; Sa, Tong-Min

    2007-02-01

    A pink-pigmented, facultatively methylotrophic bacterium, strain CBMB20T, isolated from stem tissues of rice, was analysed by a polyphasic approach. Strain CBMB20T utilized 1-aminocyclopropane 1-carboxylate (ACC) as a nitrogen source and produced ACC deaminase. It was related phylogenetically to members of the genus Methylobacterium. 16S rRNA gene sequence analysis indicated that strain CBMB20T was most closely related to Methylobacterium fujisawaense, Methylobacterium radiotolerans and Methylobacterium mesophilicum; however, DNA-DNA hybridization values were less than 70 % with the type strains of these species. The DNA G+C content of strain CBMB20T was 70.6 mol%. The study presents a detailed phenotypic characterization of strain CBMB20T that allows its differentiation from other Methylobacterium species. In addition, strain CBMB20T is the only known member of the genus Methylobacterium to be described from the phyllosphere of rice. Based on the data presented, strain CBMB20T represents a novel species in the genus Methylobacterium, for which the name Methylobacterium oryzae sp. nov. is proposed, with strain CBMB20T (=DSM 18207T=LMG 23582T=KACC 11585T) as the type strain.

  19. The abundance of pink-pigmented facultative methylotrophs in the root zone of plant species in invaded coastal sage scrub habitat.

    Directory of Open Access Journals (Sweden)

    Irina C Irvine

    Full Text Available Pink-pigmented facultative methylotrophic bacteria (PPFMs are associated with the roots, leaves and seeds of most terrestrial plants and utilize volatile C(1 compounds such as methanol generated by growing plants during cell division. PPFMs have been well studied in agricultural systems due to their importance in crop seed germination, yield, pathogen resistance and drought stress tolerance. In contrast, little is known about the PPFM abundance and diversity in natural ecosystems, let alone their interactions with non-crop species. Here we surveyed PPFM abundance in the root zone soil of 5 native and 5 invasive plant species along ten invasion gradients in Southern California coastal sage scrub habitat. PPFMs were present in every soil sample and ranged in abundance from 10(2 to 10(5 CFU/g dry soil. This abundance varied significantly among plant species. PPFM abundance was 50% higher in the root zones of annual or biennial species (many invasives than perennial species (all natives. Further, PPFM abundance appears to be influenced by the plant community beyond the root zone; pure stands of either native or invasive species had 50% more PPFMs than mixed species stands. In sum, PPFM abundance in the root zone of coastal sage scrub plants is influenced by both the immediate and surrounding plant communities. The results also suggest that PPFMs are a good target for future work on plant-microorganism feedbacks in natural ecosystems.

  20. A Near Infrared Spectroscopy (NIRS) and Chemometric Approach to Improve Apple Fruit Quality Management: A Case Study on the Cultivars "Cripps Pink" and "Braeburn".

    Science.gov (United States)

    Eisenstecken, Daniela; Panarese, Alessia; Robatscher, Peter; Huck, Christian W; Zanella, Angelo; Oberhuber, Michael

    2015-07-24

    The potential of near infrared spectroscopy (NIRS) in the wavelength range of 1000-2500 nm for predicting quality parameters such as total soluble solids (TSS), acidity (TA), firmness, and individual sugars (glucose, fructose, sucrose, and xylose) for two cultivars of apples ("Braeburn" and "Cripps Pink") was studied during the pre- and post-storage periods. Simultaneously, a qualitative investigation on the capability of NIRS to discriminate varieties, harvest dates, storage periods and fruit inhomogeneity was carried out. In order to generate a sample set with high variability within the most relevant apple quality traits, three different harvest time points in combination with five different storage periods were chosen, and the evolution of important quality parameters was followed both with NIRS and wet chemical methods. By applying a principal component analysis (PCA) a differentiation between the two cultivars, freshly harvested vs. long-term stored apples and, notably, between the sun-exposed vs. shaded side of apples could be found. For the determination of quality parameters effective prediction models for titratable acid (TA) and individual sugars such as fructose, glucose and sucrose by using partial least square (PLS) regression have been developed. Our results complement earlier reports, highlighting the versatility of NIRS as a fast, non-invasive method for quantitative and qualitative studies on apples.

  1. Importance of seagrass-mangrove continuum as feeding grounds for juvenile pink ear emperor Lethrinus lentjan in Setiu Lagoon, Malaysia: Stable isotope approach

    Science.gov (United States)

    Le, Dung Quang; Tanaka, Kentaro; Hii, Yii Siang; Sano, Yuji; Nanjo, Kusuto; Shirai, Kotaro

    2018-05-01

    The commercially important pink ear emperor fish, Lethrinus lentjan, often occurs as a juvenile in subtropical and tropical interlinked mangrove and seagrass ecosystems, but little is known about its feeding habits and habitat use. Here, we used gut contents and stable isotopic (δ13C and δ15N) ratios to determine temporal changes in food sources and foraging habits of juvenile and sub-adult fish collected in mangrove forests and seagrass beds in the Setiu Lagoon. Gut content examination identified the main food sources as crustaceans, gastropods, bivalves, and annelids. Stable isotope analysis of food sources showed marked differences between the mangroves (δ13C = -26.8 ± 2.0‰; δ15N = 4.3 ± 1.7‰) and the seagrasses (mean ± S.D. δ13C = -20.5 ± 5.5‰; δ15N = 5.8 ± 1.2‰). The isotopic composition of L. lentjan revealed that it mainly utilized seagrass-based food sources. Rainfall and the semi-diurnal tidal regimes may affect the foraging habitats of fish in the lagoon. A significant depletion of 13C related to body size was observed, suggesting that mangroves provided some benefits to sub-adult fish. In contrast, trophic position increased with fish growth, although this increase was negligible. A stable isotope mixing model confirmed that the seagrass bed constituted the main carbon source for the fish, but with an increasing contribution of mangrove prey, such as sesarmid crabs, related to fish growth. This study provided novel information on seasonal variations in feeding areas and flexible habitat use in L. lentjan in the Setiu Lagoon, which will help optimize management strategies for sustainable use and wildlife conservation.

  2. What We Have Learned and What We Have Missed in Tuberculosis Pathophysiology for a New Vaccine Design: Searching for the “Pink Swan”

    Directory of Open Access Journals (Sweden)

    Pere-Joan Cardona

    2017-05-01

    Full Text Available This is a call to encourage the search for a new vaccine to stop the progression of Mycobacterium tuberculosis infection to tuberculosis (TB disease. TB is a highly discreet and stigmatized disease, with a massive impact on human health. It has killed 1.2 billion people in the last 200 years and still kills 1.5 million people per year. Over the last 20 years, the TB vaccine field has experienced spectacular developments, and we have learned about (1 the importance of the Th1 response in controlling infection, mainly against RD1 and Ag85 antigens; (2 the stability of the antigenic repertoire; (3 the dynamics of M. tuberculosis granulomas; or (4 the link between typical and atypical pulmonary TB and the immune status of the host. However, we still do not (1 know how to avoid M. tuberculosis infection and reinfection; (2 understand the major role of the increase in lesion size in progression from infection to disease; (3 the role of interlobular septa in encapsulating pulmonary lesions; or (4 the role of neutrophilic infiltration and an exaggerated inflammatory response in the development of TB disease. These are strong reasons to pursue new, imaginative proposals involving both the antibody response and a balanced, tolerant immune response that averts progression toward TB. So far, the scientific mindset has been quite monolithic and has mainly focused on the stimulation of conventional T cells. But this approach has failed. For that reason, we are seeking unconventional perspectives to find a “pink swan,” a more efficacious and safer vaccine candidate.

  3. What We Have Learned and What We Have Missed in Tuberculosis Pathophysiology for a New Vaccine Design: Searching for the "Pink Swan".

    Science.gov (United States)

    Cardona, Pere-Joan

    2017-01-01

    This is a call to encourage the search for a new vaccine to stop the progression of Mycobacterium tuberculosis infection to tuberculosis (TB) disease. TB is a highly discreet and stigmatized disease, with a massive impact on human health. It has killed 1.2 billion people in the last 200 years and still kills 1.5 million people per year. Over the last 20 years, the TB vaccine field has experienced spectacular developments, and we have learned about (1) the importance of the Th1 response in controlling infection, mainly against RD1 and Ag85 antigens; (2) the stability of the antigenic repertoire; (3) the dynamics of M. tuberculosis granulomas; or (4) the link between typical and atypical pulmonary TB and the immune status of the host. However, we still do not (1) know how to avoid M. tuberculosis infection and reinfection; (2) understand the major role of the increase in lesion size in progression from infection to disease; (3) the role of interlobular septa in encapsulating pulmonary lesions; or (4) the role of neutrophilic infiltration and an exaggerated inflammatory response in the development of TB disease. These are strong reasons to pursue new, imaginative proposals involving both the antibody response and a balanced, tolerant immune response that averts progression toward TB. So far, the scientific mindset has been quite monolithic and has mainly focused on the stimulation of conventional T cells. But this approach has failed. For that reason, we are seeking unconventional perspectives to find a "pink swan," a more efficacious and safer vaccine candidate.

  4. What We Have Learned and What We Have Missed in Tuberculosis Pathophysiology for a New Vaccine Design: Searching for the “Pink Swan”

    Science.gov (United States)

    Cardona, Pere-Joan

    2017-01-01

    This is a call to encourage the search for a new vaccine to stop the progression of Mycobacterium tuberculosis infection to tuberculosis (TB) disease. TB is a highly discreet and stigmatized disease, with a massive impact on human health. It has killed 1.2 billion people in the last 200 years and still kills 1.5 million people per year. Over the last 20 years, the TB vaccine field has experienced spectacular developments, and we have learned about (1) the importance of the Th1 response in controlling infection, mainly against RD1 and Ag85 antigens; (2) the stability of the antigenic repertoire; (3) the dynamics of M. tuberculosis granulomas; or (4) the link between typical and atypical pulmonary TB and the immune status of the host. However, we still do not (1) know how to avoid M. tuberculosis infection and reinfection; (2) understand the major role of the increase in lesion size in progression from infection to disease; (3) the role of interlobular septa in encapsulating pulmonary lesions; or (4) the role of neutrophilic infiltration and an exaggerated inflammatory response in the development of TB disease. These are strong reasons to pursue new, imaginative proposals involving both the antibody response and a balanced, tolerant immune response that averts progression toward TB. So far, the scientific mindset has been quite monolithic and has mainly focused on the stimulation of conventional T cells. But this approach has failed. For that reason, we are seeking unconventional perspectives to find a “pink swan,” a more efficacious and safer vaccine candidate. PMID:28555137

  5. Reproducibility of the pink esthetic score--rating soft tissue esthetics around single-implant restorations with regard to dental observer specialization.

    Science.gov (United States)

    Gehrke, Peter; Lobert, Markus; Dhom, Günter

    2008-01-01

    The pink esthetic score (PES) evaluates the esthetic outcome of soft tissue around implant-supported single crowns in the anterior zone by awarding seven points for the mesial and distal papilla, soft-tissue level, soft-tissue contour, soft-tissue color, soft-tissue texture, and alveolar process deficiency. The aim of this study was to measure the reproducibility of the PES and assess the influence exerted by the examiner's degree of dental specialization. Fifteen examiners (three general dentists, three oral maxillofacial surgeons, three orthodontists, three postgraduate students in implant dentistry, and three lay people) applied the PES to 30 implant-supported single restorations twice at an interval of 4 weeks. Using a 0-1-2 scoring system, 0 being the lowest, 2 being the highest value, the maximum achievable PES was 14. At the second assessment, the photographs were scored in reverse order. Differences between the two assessments were evaluated with the Spearman's rank correlation coefficient (R). The Wilcoxon signed-rank test was used for comparisons of differences between the ratings. A significance level of p esthetic restorations showed the smallest deviations. Orthodontists were found to have assigned significantly poorer ratings than any other group. The assessment of postgraduate students and laypersons were the most favorable. The PES allows for a more objective appraisal of the esthetic short- and long-term results of various surgical and prosthetic implant procedures. It reproducibly evaluates the peri-implant soft tissue around single-implant restorations and results in good intra-examiner agreement. However, an effect of observer specialization on rating soft-tissue esthetics can be shown.

  6. Different roles suggested by sex-biased expression and pheromone binding affinity among three pheromone binding proteins in the pink rice borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Jin, Jun-Yan; Li, Zhao-Qun; Zhang, Ya-Nan; Liu, Nai-Yong; Dong, Shuang-Lin

    2014-07-01

    Pheromone binding proteins (PBPs) are thought to bind and transport hydrophobic sex pheromone molecules across the aqueous sensillar lymph to specific pheromone receptors on the dendritic membrane of olfactory neurons. A maximum of 3 PBP genes have been consistently identified in noctuid species, and each of them shares high identity with its counterparts in other species within the family. The functionality differences of the 3 proteins are poorly understood. In the present study, 3 PBP cDNAs (SinfPBP1, 2, 3) were identified from the pink rice borer, Sesamia inferens, for the first time. The quantitative real-time PCR indicated that the 3 PBPs displayed similar temporal but very different sex related expression profiles. Expression of SinfPBP1 and SinfPBP2 were highly and moderately male biased, respectively, while SinfPBP3 was slightly female biased, as SinfPBPs were expressed at very different levels (PBP1>PBP2≫PBP3) in male antennae, but at similar levels in female antennae. Furthermore, the 3 SinfPBPs displayed different ligand binding profiles in fluorescence competitive binding assays. SinfPBP1 exhibited high and similar binding affinities to all 3 sex pheromone components (Ki=0.72-1.60 μM), while SinfPBP2 showed selective binding to the alcohol and aldehyde components (Ki=0.78-1.71 μM), and SinfPBP3 showed no obvious binding to the 3 sex pheromone components. The results suggest that SinfPBP1 plays a major role in the reception of female sex pheromones in S. inferens, while SinfPBP3 plays a least role (if any) and SinfPBP2 functions as a recognizer of alcohol and aldehyde components. Copyright © 2014 Elsevier Ltd. All rights reserved.

  7. Isolation and characterization of Rhodococcus ruber CGMCC3090 ...

    African Journals Online (AJOL)

    A bacterial strain was isolated from soil samples that had been polluted by nitrile compounds. This strain converts acrylonitrile to acrylamide with high activity. The nitrile hydrolysis activity was tested using eight substrates, including aliphatic, aromatic and heterocyclic (di)nitriles. All of the nitrile compounds were hydrolyzed ...

  8. Rufibacter ruber sp. nov., isolated from fragmentary rock

    Czech Academy of Sciences Publication Activity Database

    Kýrová, K.; Sedláček, I.; Pantůček, R.; Králová, S.; Holochová, P.; Mašlaňová, I.; Staňková, E.; Kleinhagauer, T.; Gelbíčková, T.; Sobotka, Roman; Švec, P.; Busse, H.-J.

    2016-01-01

    Roč. 66, č. 11 (2016), s. 4401-4405 ISSN 1466-5026 R&D Projects: GA MŠk(CZ) LO1416 Institutional support: RVO:61388971 Keywords : Gram-stain-negative * CCM 8646T * Rufibacter Subject RIV: EE - Microbiology, Virology Impact factor: 2.134, year: 2016

  9. Isolation and characterization of Rhodococcus ruber CGMCC3090 ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... Available online at http://www.academicjournals.org/AJB. ISSN 1684–5315 .... the soil samples by an enrichment culture method in basal medium containing 0.5 ..... The phylogeny of the genus Rhodococcus currently remains ...

  10. Philopatry in a changing world: Response of Pink-footed Geese Anser brachyrhynchus to the loss of a key autumn staging area due to restoration of Filsø Lake, Denmark

    DEFF Research Database (Denmark)

    Clausen, Kevin; Madsen, Jesper

    2016-01-01

    to the sudden loss of a major autumn staging area along their migration corridor, Filsø in Denmark, which followed the re-establishment of a former lake on open arable land serving as foraging site to tens of thousands of geese. Comparisons of goose usage before and after the restoration event revealed that 1...... philopatric to the Filsø area was unaffected by these changes, suggesting that geese quickly moved to other areas and responded well to the sudden decline in available food at their formerly preferred staging site. These findings indicate that at least for Pink-footed Geese the cognitive plasticity necessary...

  11. Conjunctivitis (Pink Eye) in Newborns

    Science.gov (United States)

    ... as well as the sexually transmitted infection called gonorrhea. Women with untreated gonorrhea can pass the bacteria to her baby during ... conjunctivitis is less common than conjunctivitis caused by gonorrhea and chlamydia. Symptoms usually include red eye(s) and ...

  12. A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources.

    Science.gov (United States)

    Wood, A P; Kelly, D P; McDonald, I R; Jordan, S L; Morgan, T D; Khan, S; Murrell, J C; Borodina, E

    1998-02-01

    The isolation and properties of a novel species of pink-pigmented methylotroph, Methylobacterium thiocyanatum, are described. This organism satisfied all the morphological, biochemical, and growth-substrate criteria to be placed in the genus Methylobacterium. Sequencing of the gene encoding its 16S rRNA confirmed its position in this genus, with its closest phylogenetic relatives being M. rhodesianum, M. zatmanii and M. extorquens, from which it differed in its ability to grow on several diagnostic substrates. Methanol-grown organisms contained high activities of hydroxypyruvate reductase -3 micromol NADH oxidized min-1 (mg crude extract protein)-1], showing that the serine pathway was used for methylotrophic growth. M. thiocyanatum was able to use thiocyanate or cyanate as the sole source of nitrogen for growth, and thiocyanate as the sole source of sulfur in the absence of other sulfur compounds. It tolerated high concentrations (at least 50 mM) of thiocyanate or cyanate when these were supplied as nitrogen sources. Growing cultures degraded thiocyanate to produce thiosulfate as a major sulfur end product, apparently with the intermediate formation of volatile sulfur compounds (probably hydrogen sulfide and carbonyl sulfide). Enzymatic hydrolysis of thiocyanate by cell-free extracts was not demonstrated. Cyanate was metabolized by means of a cyanase enzyme that was expressed at approximately sevenfold greater activity during growth on thiocyanate [Vmax 634 +/- 24 nmol NH3 formed min-1 (mg protein)-1] than on cyanate [89 +/- 9 nmol NH3 min-1 (mg protein)-1]. Kinetic study of the cyanase in cell-free extracts showed the enzyme (1) to exhibit high affinity for cyanate (Km 0.07 mM), (2) to require bicarbonate for activity, (3) to be subject to substrate inhibition by cyanate and competitive inhibition by thiocyanate (Ki 0.65 mM), (4) to be unaffected by 1 mM ammonium chloride, (5) to be strongly inhibited by selenocyanate, and (6) to be slightly inhibited by 5 m

  13. PROTETOR SOLAR DIMINUI A INCIDÊNCIA DAS PODRIDÕES ‘OLHO-D-BOI’ E ‘BRANCA’ EM MAÇÃS ‘FUJI STANDARD’ E ‘PINK LADY’

    Directory of Open Access Journals (Sweden)

    ROSA MARIA VALDEBENITO-SANHUEZA

    2016-02-01

    Full Text Available RESUMO A podridão-olho-de-boi (Cryptosporiosis perennans e a podridão-branca (Botryosphaeria dothidea estão entre as principais doenças de verão da macieira no Brasil. O objetivo deste trabalho foi avaliar o efeito de uma película protetora constituída de cera de carnaúba e argilas (Raynox® , nas doses de 2,5 e 5,0%, na infecção por C. perennans e por B. dothidea em maçãs das cultivares Fuji Standard e Pink Lady. As macieiras foram pulverizadas cinco vezes de dezembro de 2007 a março de 2008, conforme previsão de condições adequadas para ocorrência dos danos e crescimento dos frutos. Na colheita, as maçãs foram inoculadas com os dois patógenos e avaliadas quanto à incidência das podridões. Indiferente à dose, o uso de Raynox® reduziu a incidência da podridão-branca e da podridão-olho-de-boi em 67% e 42% na ‘Fuji Standard’, e 43% e 42% na ‘Pink Lady’, respectivamente. O incremento da dose aumentou a eficiência do produto para o controle da podridão-olho-de-boi. Na dose de 2,5%, o controle da podridão-olho-de-boi foi de 19% e 20%, respectivamente, em maçãs ‘Fuji Standard’ e ‘Pink Lady’, enquanto para as mesmas cultivares, mas com a dose de 5,0%, estes valores foram de 65% e 63%. Mais estudos são necessários para o ajuste de dose e critérios de aplicação para que o protetor solar possa ser recomendado.

  14. Genetic and metabolic diversity of pink-pigmented facultative methylotrophs in phyllosphere of tropical plants Diversidade metabólica e genética de microrganismos metilotróficos facultativos pigmentados na filosfera de plantas tropicais

    Directory of Open Access Journals (Sweden)

    D. Balachandar

    2008-03-01

    Full Text Available Diversity of Pink-Pigmented Facultative Methylotrophs (PPFMs in phyllosphere of cotton, maize and sunflower was determined based on differential carbon-substrate utilization profile and Random Amplified Polymorphic DNA data. Results indicate that six diversified groups of PPFMs are found in these crops. Sunflower and maize phyllosphere harbor four different groups of methylobacteria while cotton has only two groups.A diversidade de microrganismos metilotróficos facultativos pigmentados (PPFMs na filosfera de algodão, milho e girassol foi determinada baseada no perfil diferencial de utilização de substratos de carbono e em dados de RAPD. Os resultados indicaram a existência de seis grupos diferentes de PPFMs nessas plantas. As filosferas de girassol e milho apresentaram quatro grupos diferentes de metilobactérias enquanto a de algodão apresentou apenas dois grupos.

  15. High - Resolution SST Record Based on Mg/Ca Ratios of Late Holocene Planktonic Foraminifers From the Great Bahama Bank

    Science.gov (United States)

    Mueller, A.; Reijmer, J. J.; Roth, S.

    2001-12-01

    We analyzed five different planktic foraminifera species in the high resolution core MD 992201 off the Great Bahama Bank (79° 16.34 W; 25° 53.49 N) in 290 m water depth. This 38.05 m long core comprises a 7,000 year long Holocene record. The selected species were Orbulina universa, Globigerinoides ruber, Globigerinoides sacculifer, Globorotalia menardii and Globigerinella aequilateralis, which live in the upper 200 m of the water column. The Mg/Ca ratios of these different foraminifers show species-specific values, which represent a distinct habitat depth. With this species-specific Mg/Ca ratios we can reconstruct a temperature profile through the water column. The lowest Mg/Ca are shown by G. menardii (2.5 - 4 mmol/mol), followed by G. sacculifer (4.2 - 5.6 mmol/mol), G. ruber (5.1 - 7.2 mmol/mol) and G. aequilateralis (5.5 - 8.7 mmol/mol). Highest are shown by O. universa (6 - 14 mmol/mol). During the Little Ice Age, the Mg/Ca ratios of all species except for the deeper dwelling G. menardii, became more variable and showed lower ratios. The shallow dwelling species like G. ruber and G. sacculifer display an increase in the Mg/Ca ratios during the Medieval Warm Period. Our data show that transferring Mg/Ca ratios into SST based calibration curves known from literature needs re-evaluation. Species-specific calibration seems to be necessary to achieve reliable results.

  16. Post harvest of pink ginger floral stems treated with silver thiosulphate, sucrose, and calcium Pós-colheita de hastes de alpínia tratadas com tiossulfato de prata, sacarose e cálcio

    Directory of Open Access Journals (Sweden)

    Antonio Tarciso C da Silva

    2009-09-01

    Full Text Available The Brazilian market of tropical flowers has been growing broadly with a strong participation of the Northeast Region, where the State of Alagoas stands out. Among the exporting tropical cut flowers, pink ginger (Alpinia purpurata (Vieill K. Schum has been one of the most promising species. Aiming at delaying senescence in floral stems of pink ginger, two laboratory experiments were carried out at the Agricultural Science Center of the Federal University of Alagoas. In the first experiment, three different exposure periods (30; 60, and 120 minutes to silver thiosulphate 1 mM were tested, followed or not by pulsing in a 20% sucrose solution, for 12 hours. In the second experiment, we evaluated the effect of adding calcium sulphate 50 and 100 mM, sodium silicate 1.25 and 2.5 mM, and the combination of calcium sulphate 50mM + sodium silicate 1.25mM on the hydric status and longevity of floral stems. In both experiments, the control treatment consisted of keeping stems in distilled water. Fresh biomass and quality of floral stems were determined every other day. Silver thiosulphate applied in pulsing for 60 minutes or more led to stem dehydration, whereas calcium sulphate improved both stem hydration and commercial durability.O mercado brasileiro de flores tropicais encontra-se em franco crescimento, com uma importante participação da região Nordeste, destacando-se o estado de Alagoas. Dentre as flores tropicais de corte para exportação, a alpínia (Alpiniapurpurata (Vieill K. Schum ocupa posição de destaque. Visando retardar a senescência das hastes florais de alpínia, cultivar Pink Ginger, foram conduzidos dois experimentos em laboratório no CCA da Universidade Federal de Alagoas. No primeiro, foram testados três tempos (30; 60 e 120 minutos de exposição ao tiossulfato de prata 1 mM, seguido ou não de pulsing em sacarose a 20%, por 12 horas. No segundo experimento foram verificados os efeitos nas relações hídricas e longevidade

  17. Efeito do processamento sobre os níveis de colesterol e 7-cetocolesterol em camarão-rosa Effects of processing on free cholesterol and 7-ketocholesterol concentrations in pink-shrimp

    Directory of Open Access Journals (Sweden)

    Andréa Figueiredo Procópio de Moura

    2002-08-01

    has been used as a "tracer" of the degree of cholesterol oxidation, due to its fast and continuous formation and thus relatively high amounts with respect to the other oxidation products. The objective of this work was to evaluate the effects of boiling and deep-frying on the cholesterol oxidation in pink-shrimp (Penaeus brasiliensis and Penaeus paulensis. Cooking of pink-shrimp, in general, decreased both free cholesterol and 7-ketocholesterol concentrations. Such decrease was higher after frying than after boiling and was more pronounced for 7-ketocholesterol. While the cholesterol concentrations were 10.7 and 24.7% lower, the decrease of 7-ketocholesterol concentrations was 41.5 and 61% in boiled and fried shrimp, respectively. The reduction of free cholesterol and 7-ketocholesterol concentrations in processed pink-shrimp was related to elution of these compounds by the cooking medium, i.e. water in boiling and oil in frying.

  18. Evaluation of PWR and BWR assembly benchmark calculations. Status report of EPRI computational benchmark results, performed in the framework of the Netherlands` PINK programme (Joint project of ECN, IRI, KEMA and GKN)

    Energy Technology Data Exchange (ETDEWEB)

    Gruppelaar, H. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Klippel, H.T. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Kloosterman, J.L. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Hoogenboom, J.E. [Technische Univ. Delft (Netherlands). Interfacultair Reactor Instituut; Leege, P.F.A. de [Technische Univ. Delft (Netherlands). Interfacultair Reactor Instituut; Verhagen, F.C.M. [Keuring van Electrotechnische Materialen NV, Arnhem (Netherlands); Bruggink, J.C. [Gemeenschappelijke Kernenergiecentrale Nederland N.V., Dodewaard (Netherlands)

    1993-11-01

    Benchmark results of the Dutch PINK working group on calculational benchmarks on single pin cell and multipin assemblies as defined by EPRI are presented and evaluated. First a short update of methods used by the various institutes involved is given as well as an update of the status with respect to previous performed pin-cell calculations. Problems detected in previous pin-cell calculations are inspected more closely. Detailed discussion of results of multipin assembly calculations is given. The assembly consists of 9 pins in a multicell square lattice in which the central pin is filled differently, i.e. a Gd pin for the BWR assembly and a control rod/guide tube for the PWR assembly. The results for pin cells showed a rather good overall agreement between the four participants although BWR pins with high void fraction turned out to be difficult to calculate. With respect to burnup calculations good overall agreement for the reactivity swing was obtained, provided that a fine time grid is used. (orig.)

  19. Evaluation of PWR and BWR assembly benchmark calculations. Status report of EPRI computational benchmark results, performed in the framework of the Netherlands' PINK programme (Joint project of ECN, IRI, KEMA and GKN)

    International Nuclear Information System (INIS)

    Gruppelaar, H.; Klippel, H.T.; Kloosterman, J.L.; Hoogenboom, J.E.; Bruggink, J.C.

    1993-11-01

    Benchmark results of the Dutch PINK working group on calculational benchmarks on single pin cell and multipin assemblies as defined by EPRI are presented and evaluated. First a short update of methods used by the various institutes involved is given as well as an update of the status with respect to previous performed pin-cell calculations. Problems detected in previous pin-cell calculations are inspected more closely. Detailed discussion of results of multipin assembly calculations is given. The assembly consists of 9 pins in a multicell square lattice in which the central pin is filled differently, i.e. a Gd pin for the BWR assembly and a control rod/guide tube for the PWR assembly. The results for pin cells showed a rather good overall agreement between the four participants although BWR pins with high void fraction turned out to be difficult to calculate. With respect to burnup calculations good overall agreement for the reactivity swing was obtained, provided that a fine time grid is used. (orig.)

  20. The effect of silicon foliar application on the development of Season ornamental plants. Part II: Argyranthemum frutescens 'Blazer Rose', Xerochrysum bracteatum 'Gold', Osteospermum ecklonis 'Grande Pink Blush' and Gaura lindheimeri 'Corinas Choice'

    Directory of Open Access Journals (Sweden)

    Katarzyna Wróblewska

    2012-12-01

    Full Text Available Recent research has proved that supplementary application of silicon, involved in plant tolerance against many stress factors, positively influences plant development improving the quality of ornamental plants. A two-factorial experiment involved four cultivars of ornamental plants: Argyranthemum frutescens 'Blazer Rose', Xerochrysum bracteatum 'Gold', Osteospermum ecklonis 'Grande Pink Blush', and Gaura lindheimeri 'Corinas Choice' The first experimental factor was the concentration of Actisil preparation being an equivalent of 60, 120, and 180 mg Si×dm-3, applied three times by spraying, while the second one was the type of medium: peat substrate and peat substrate with sand. Actisil had a beneficial effect on the number of lateral shoots in all plants. The highest number of buds and inflorescences was determined in Xerochrysum and Osteospermum plants sprayed with 0.3% Actisil, and 0.2% Actisil in case of Argyranthemum and Gaura. The effect of medium type on plant growth depended on plant species. Argyranthemum and Osteospermum plants cultivated in the medium with sand showed inhibited growth, although medium type did not affect the growth of Xerochrysum.

  1. Qualidade química e microbiológica de camarão-rosa comercializado em São Paulo Chemical and microbiological quality of pink shrimp sold in São Paulo

    Directory of Open Access Journals (Sweden)

    Andréa Figueiredo Procópio de Moura

    2003-06-01

    Full Text Available Durante as etapas de captura, manipulação e distribuição, o camarão precisa receber tratamentos adequados visando à manutenção das condições apropriadas de consumo. Com o intuito de avaliar a qualidade de camarão-rosa comercializado no município de São Paulo, amostras do crustáceo foram submetidas a testes de bases voláteis totais (BVT, substâncias reativas ao ácido tiobarbitúrico (TBARs, pH e análises microbiológicas (contagens totais de mesófilos e psicrotróficos. Estas provas, analisadas em conjunto, são consideradas parâmetros adequados no reconhecimento da qualidade do pescado. As amostras analisadas apresentaram valores de pH de 7,09 a 8,10, BVT entre 27,6 e 73 mgN/100 g, TBARs de 0,17 a 2,12 mg aldeído malônico/kg, contagem total de mesófilos de 2,5 x 10³ a 1,7 x 10(7 UFC/g e contagem total de psicrotróficos variando de 1,1 x 10(4 a 3,0 x 10(7 UFC/g. Os resultados obtidos sugerem qualidade química insatisfatória para o camarão-rosa analisado, incompatível para um produto considerado comercialmente como fresco.It is necessary to take special care when catching, handling and distributing shrimps in order to keep them in adequate conditions for future consumption. The objective of this study was to evaluate the quality of pink shrimp sold in São Paulo, Brazil. Samples of this crustacean were submitted to VBN (Volatile Basic Nitrogen, TBARS (Thiobarbituric Acid-Reactive Substances, pH and microbial analyses (Mesophilic and Psychrotrophic Plate Counts. These tests together are suitable parameters to evaluate the fish quality. The analyses of the samples showed the following results: the pH values ranged from 7.09 to 8.10, the VBN values ranged from 27.6 to 73 mgN/100 g, the TBARS values ranged from 0.17 to 2.12 mg malonaldehyde/kg, the Mesophilic Plate Counts ranged from 2.5 x 10³ to 1.7 x 10(7 UFC/g, and the Psychrotrophic Plate Counts ranged from 1.1 x 10(4 to 3.0 x 10(7 UFC/g. The results suggest that the

  2. Quaternary carbonate record from the equatorial Indian Ocean and its relationship with productivity changes

    Digital Repository Service at National Institute of Oceanography (India)

    Naidu, P.D.; Malmgren, B.A.

    Globigerinoides ruber. This indicates that CaCO fluctuations at this site do not show either a 3 y2 y1 .Pacific or an Atlantic pattern of CaCO fluctuations. Fluxes of CaCO 0.38 to 2.46 g cm ka in total sediment and 33 y2 y1 .BarAl ratios 0.58 to 3.93 g cm ka... show six-fold variability through the last 1370 ka, which points out that productivity changes are significant at this site. Fluxes of the fine CaCO component demonstrate a 26-fold change 0.02 to 3 y2 y1 . y2 y1 .0.52 g cm ka , whereas the coarse Ca...

  3. Millennial- to century-scale variability in Gulf of Mexico Holocene climate records

    Science.gov (United States)

    Poore, R.Z.; Dowsett, H.J.; Verardo, S.; Quinn, T.M.

    2003-01-01

    Proxy records from two piston cores in the Gulf of Mexico (GOM) provide a detailed (50-100 year resolution) record of climate variability over the last 14,000 years. Long-term (millennial-scale) trends and changes are related to the transition from glacial to interglacial conditions and movement of the average position of the Intertropical Convergence Zone (ITCZ) related to orbital forcing. The ??18O of the surface-dwelling planktic foraminifer Globigerinoides ruber show negative excursions between 14 and 10.2 ka (radiocarbon years) that reflect influx of meltwater into the western GOM during melting of the Laurentide Ice Sheet. The relative abundance of the planktic foraminifer Globigerinoides sacculifer is related to transport of Caribbean water into the GOM. Maximum transport of Caribbean surface waters and moisture into the GOM associated with a northward migration of the average position of the ITCZ occurs between about 6.5 and 4.5 ka. In addition, abundance variations of G. sacculifer show century-scale variability throughout most of the Holocene. The GOM record is consistent with records from other areas, suggesting that century-scale variability is a pervasive feature of Holocene climate. The frequency of several cycles in the climate records is similar to cycles identified in proxy records of solar variability, indicating that at least some of the century-scale climate variability during the Holocene is due to external (solar) forcing.

  4. Salinity controls on Na incorporation in Red Sea planktonic foraminifera

    Science.gov (United States)

    Mezger, E. M.; de Nooijer, L. J.; Boer, W.; Brummer, G. J. A.; Reichart, G. J.

    2016-12-01

    Whereas several well-established proxies are available for reconstructing past temperatures, salinity remains challenging to assess. Reconstructions based on the combination of (in)organic temperature proxies and foraminiferal stable oxygen isotopes result in relatively large uncertainties, which may be reduced by application of a direct salinity proxy. Cultured benthic and planktonic foraminifera showed that Na incorporation in foraminiferal shell calcite provides a potential independent proxy for salinity. Here we present the first field calibration of such a potential proxy. Living planktonic foraminiferal specimens from the Red Sea surface waters were collected and analyzed for their Na/Ca content using laser ablation quadrupole inductively coupled plasma mass spectrometry. Using the Red Sea as a natural laboratory, the calibration covers a broad range of salinities over a steep gradient within the same water mass. For both Globigerinoides ruber and Globigerinoides sacculifer calcite Na/Ca increases with salinity, albeit with a relatively large intraspecimen and interspecimen variability. The field-based calibration is similar for both species from a salinity of 36.8 up to 39.6, while values for G. sacculifer deviate from this trend in the northernmost transect. It is hypothesized that the foraminifera in the northernmost part of the Red Sea are (partly) expatriated and hence should be excluded from the Na/Ca-salinity calibration. Incorporation of Na in foraminiferal calcite therefore provides a potential proxy for salinity, although species-specific calibrations are still required and more research on the effect of temperature is needed.

  5. Late Holocene hydrographic settings of the northern Red Sea

    Directory of Open Access Journals (Sweden)

    Amani Badawi

    2016-03-01

    Full Text Available Temporal variability of the paleo-oceanographic setting of the northern Red Sea during the last 6 Ky was deduced from high-resolution faunal results and stable isotope records of planktonic foraminifera in three short cores sediment obtained by the German R/V Meteor vessel. In general, the investigated time interval is fundamentally comparable to the present day composition and distribution of planktonic foraminifera. However, interrupted short enhanced arid phase spanning the last 4–2 Ky appears to have existed in the northern Red Sea, and resulted in elevation of salinity and somehow productivity, as hypersaline, dense surface water favored vertical mixing of the water column resulting in an increase in productivity. This paleoclimatic reconstruction is revealed from the distinct gradient in the composition and distribution of planktonic foraminifera, as well as the significant distribution trend of Globigerinoides ruber versus Globigerinoides sacculifer correlated with the stable isotope records. Starting from the last 2 Ky to the present time, less strength arid conditions relative to the previous period prevailed, reflected from a gradual decrease in surface water salinity and productivity assuming that the present water conditions and consequently current climatic conditions began to develop from that time with minor fluctuations reaching the recent conditions.

  6. Middle Pleistocene Transition (MPT) in the Eastern Indian Ocean: a 2000 kyr planktic faunal and isotope record from DSDP site 214

    International Nuclear Information System (INIS)

    Gupta, Anil K.; Dhingra, Hitesh

    2004-01-01

    Planktic foraminiferal faunal and isotope data from Deep Sea Drilling Project (DSDP) Site 214 reveal a major change in surface water properties in the eastern Indian Ocean, coinciding with the mid-Pleistocene climate transition (MPT). A comparative study of Globigerinoides sacculifer (a surface dwelling, warm water, mixed layer tropical planktic foraminifera), Globorotalia menardii Complex (a deep dwelling, tropical species group), and Orbulina universa (an intermediate depth warm-water subtropical foraminifera) with the stable isotope record of Globigerinoides ruber suggests a warm, thick mixed layer in the eastern Indian Ocean during,∼ 2000 Kyr to ∼ 900 Kyr. Since,∼ 900 Kyr the surface water mass stratification weakened, and the mixed layer as well as thermocline were shallow. A decrease in the population abundance of Gs. sacculifer, together with a decrease in δ 13 C and increase in δ 18 O values suggest a continuous cool climate and increased surface productivity over the last ∼ 900 Kyr. This coincides with an increased variance in the 400 ∼Kyr component of Earth's eccentricity cycle. (author)

  7. Pink eye in potato is problematic

    Science.gov (United States)

    Fall of 2017 was a challenging harvest season for potato growers in Central Wisconsin because of the less-than-ideal patterns of temperature and precipitation. Late September had several days with high temperatures above 85'F that shut down most harvest operations. Early October brought in quite a f...

  8. Reconstruction of pink esthetics: The periodontal way

    Directory of Open Access Journals (Sweden)

    K Balasubramanian

    2015-01-01

    Full Text Available Cosmetic procedures involving gingival reconstruction have become an integral part of current periodontal practice. The ability to cover unsightly exposed, sensitive roots and recontour soft tissue recessions have added an esthetic angle to the traditional concept of biological and functional periodontal health. The recession of the gingiva, either localized or generalized, may be associated with one or more surfaces, resulting in attachment loss and root exposure, which can lead to clinical problems such as diminished cosmetic appeal and aesthetic concern. Marginal gingival recession, therefore, can cause major functional and aesthetic problems and should not be viewed as merely a soft tissue defect, but rather as the destruction of both the soft and hard tissue. Treatment proposals for this type of defect have evolved based on the knowledge for healing the gingiva and the attachment system. This case report describes a clinical case of severe Miller Class II gingival recession treated by two stages of surgery that combined a free gingival graft and connective tissue grafting. First, a free gingival graft (FGG was performed to obtain an adequate keratinized tissue level. Three months later, a connective tissue graft (CTGwas performed to obtain root coverage. The results indicated that the FGG allows for a gain in the keratinized tissue level and the CTG allows for root coverage with decreased recession level after 6 months. Therefore, for this type of specific gingival recession, the combined use of FGG and CTG still serves as a Gold Standard in predictable root coverage.

  9. [Perfecting smile esthetics: keep it pink!

    Science.gov (United States)

    Monnet-Corti, Virginie; Antezack, Angeline; Pignoly, Marion

    2018-03-01

    Smile aesthetics is based on numerical, physical, physiological and psychological data regarding beauty, while taking into account the desires of the patient. It is determined by the shape, colour and position of the lips, teeth and gingival tissues. Periodontal examination in both the facial and labial settings supports analysis of the gingival display during natural and forced smiling, the health of the periodontium, the gingival contours, the aesthetic gingival line, and the presence of the papillae. All these data will help establish a gingival aesthetic score to determine the causes of disharmony and possible treatment. During implementation of the global orthodontic treatment plan, periodontal plastic surgery can change the gingival appearance and morphology in order to restore the harmony of the smile. Subtractive periodontal plastic surgery treats biological space defects and excess tissue during incomplete passive eruption by gingivectomy or apically positioned flap, combined, or not, with osteoplasty or osteoectomy. Finally, injections of hyaluronic acid in the papillae can plump them up and minimize the size of any black holes. © EDP Sciences, SFODF, 2018.

  10. The Myth of Pink and Blue Brains

    Science.gov (United States)

    Eliot, Lise

    2010-01-01

    Eliot, a neuroscientist who has analyzed gender differences in children's brains, asserts that--contrary to the widely held idea that boys' and girls' brains are hardwired differently--few differences exist in the neural structures and neurochemistry of boys' and girls' brains. Actual ability differences between the genders are quite small as…

  11. Chocolate das bagas e clorose foliar: anomalia da videira causada por deficiência de boro Internal browning ("chocolate" and leaf chlorosis of the 'pink Niagara' grafe, a boron deficiency anomaly

    Directory of Open Access Journals (Sweden)

    Hugo Kuniyuki

    1985-01-01

    Full Text Available A anomalia do cultivar de videira Niagara Rosada, caracterizada por sintomas de clorose nas folhas, chocolate das bagas e cachos malformados, não é de natureza virótica, de acordo com resultados de testes de transmissão e de perpetuação. Essa anomalia, presente em áreas definidas de alguns vinhedos de Indaiatuba e Jundiaí (SP, está associada à deficiência nutricional de boro. O teor médio de boro em amostras de plantas afetadas de 'Niagara Rosada' (18 ppm no limbo e 7 ppm nos frutos foi menor do que nas de plantas normais (24 ppm no limbo e 10 ppm nos frutos das mesmas plantaçoes; um material coletado de um vinhedo bastante afetado mostrou 13 e 1 ppm respectivamente no limbo e nos frutos de plantas deficientes contra 20 e 6 ppm nos de plantas sem sintomas. Amostras de três vinhedos vizinhos sem essa anomalia apresentaram um teor médio de 35 ppm no limbo. As do porta-enxerto 'Traviú' com clorose e sem clorose mostraram 16 ppm e 43 ppm de boro respectivamente. A aplicação de bórax, nas doses de 5, 10 e 20g por planta, 1ogo após a poda de inverno, ou pulverização de ácido bórico nas concentraçoes de 0,3% e 0,6% sobre a folhagem, durante o início do florescimento e durante a fase de uva chumbinho, permitiram controlar a anomalia nas condições de campo. Foi notada resposta favorável por um ciclo vegetativo, pelo menos, indicando controle por efeito residual de uma aplicação.The 'pink Niagara' grape (V. labrusca L. x V. vinifera L. anomaly characterized by leaf chlorosis and "chocolate" (internal browning of the young fruit, at first thought to be a virus disease, has been found to be a result of boron deficiency. The anomaly was first recorded in 1974. It occurs in patches in vineyards of Indaiatuba and Jundiaí, two grape-growing counties of the State of São Paulo, Brazil. Affected plants show yellowing between the primary and secondary veins of the upper leaves and malformed clusters, with normal-sized berries and

  12. Growth and survival of pink shrimp (Farfantepenaeus paulensis postlarve in cages and pen enclosures Crescimento e sobrevivência de pós-larvas de camarão-rosa (Farfantepenaeus paulensis em gaiolas e cercados

    Directory of Open Access Journals (Sweden)

    Luciano Jensen Vaz

    2004-06-01

    Full Text Available Technology for the culture of the pink shrimp Farfantepenaeus paulensis in low-cost cages and pen enclosures was developed together with artisanal fishermen of the Patos Lagoon estuary, southern Brazil. Although the production of postlarvae (PL under laboratory conditions is well documented, survival and growth during the nursery phase cages and pen enclosures in the nature has not yet been analyzed. Therefore, the present study compared survival and growth of F. paulensis postlarvae reared either in cages or pens. Six cages (2.0 ´ 2.0 ´ 1.4 m of PVC-coated polyester with a mesh size of 1.5 mm were stocked with 800 PL26 each. To assess the effect of the substrate, a 10 cm layer of sand was added to three of these cages to simulate a pen enclosure. No difference in the mean survival rate between cages (92.2% and pens (88.7% was found (P > 0.05. However, final weight of shrimp reared in pens (1.05 ± 0.05 g was higher than those kept in cages (0.88 ± 0.12 g. The nursery phase of F. paulensis in cages and pens did not affect survival. Nevertheless, because of the higher growth rate of shrimp grown in pen enclosures, this kind of structure should be preferentially used in nursery rearing of this species.Um pacote tecnológico para o cultivo do camarão-rosa em estruturas alternativas de baixo custo, gaiolas e cercados, vem sendo aplicado em conjunto com pescadores artesanais do estuário da Lagoa dos Patos, no extremo sul do Brasil. Embora a tecnologia para o cultivo intensivo de pós-larvas (PL em laboratório já esteja bem documentada, informações referentes à sobrevivência e crescimento desses organismos na fase de berçário ainda são escassas para cercados e gaiolas no ambiente natural. Em virtude destes fatos, o objetivo do trabalho foi comparar o crescimento e a sobrevivência de pós-larvas de F. paulensis durante a fase de berçário em gaiolas e cercados no ambiente. Para o desenvolvimento do trabalho, foram utilizadas seis

  13. Variability of Indonesian Throughflow and Borneo Runoff During the Last 14 kyr

    Science.gov (United States)

    Hendrizan, Marfasran; Kuhnt, Wolfgang; Holbourn, Ann

    2017-10-01

    We present a high-resolution ( 20 to 100 years temporal resolution) reconstruction of hydrological changes in the Makassar Strait over the last 14 kyr from Core SO217-18517 retrieved off the Mahakam Delta (1°32.198'S, 117°33.756'E; 698 m water depth) during the SO217 Makassar-Java Cruise. Sea surface temperatures, based on Mg/Ca of Globigerinoides ruber and alkenone UK'37, and seawater δ18O reconstructions, based on G. ruber δ18O and Mg/Ca, in combination with sortable silt grain size measurements and X-ray fluorescence (XRF) core scanner derived elemental data provide evidence for increased precipitation during the Bølling-Allerød (BA) and early Holocene and for warmer and more saline surface waters and a decrease in the intensity of the Indonesian Throughflow (ITF) during the Younger Dryas (YD). XRF derived Log (Zr/Rb) records, sortable silt data and increased sedimentation rates indicate decreased winnowing, interpreted as a slowdown of the ITF thermocline flow during the YD. We attribute this decline in ITF intensity to slowdown of the Atlantic meridional overturning circulation during the YD. We suggest that changes in Makassar Strait surface hydrology during this interval of Northern Hemisphere cooling and Southern Hemisphere warming were related to a southward displacement of the Intertropical Convergence Zone.

  14. Paleoclimatic changes occurred during the last two centuries in the Gulf of Gaeta (central-eastern Tyrrhenian Sea): a contribution of NEXTDATA project

    Science.gov (United States)

    Lirer, Fabrizio; Vallefuoco, Mattia; Albertazzi, Sonia; Anzalone, Erlisiana; Bellucci, Luca; Bonomo, Sergio; Capotondi, Lucilla; Cascella, Antonio; Di Rita, Federico; Ferraro, Luciana; Florindo, Fabio; Giuliani, Silvia; Domenica Insinga, Donatella; Lurcock, Pont; Magri, Donatella; Margaritelli, Giulia; Marsella, Ennio; Petrosino, Paola; Sprovieri, Mario; Sorgato, Stefania

    2014-05-01

    A high-resolution integrated study, which includes tephrochronology (tefra layer associated to 1906 AD Vesuvius event) and radionuclide dating method (210Pb and 137Cs), has been performed in a super-expanded marine record (sedimentation rate of 0.47 cm/yr) from the continental shelf of the Gulf of Gaeta (central eastern Tyrrhenian Sea). Planktonic foraminifera, calcareous nannofossils, pollens and oxygen stable isotope data performed on Globigerinoides ruber, revealed environmental changes occurred during the last two centuries. In detail we recognise four paleoclimatic changes. From ca. 1800 to ca. 1865 AD, d18O G. ruber signal combined with evidences of abundance increase of planktonic foraminifera Globoratialia inflata and G. truncatulinoides and of calcareous nannofossils Emiliana huxley document cold-temperate phase. Between ca. 1865 and ca. 1882 AD, d18O G. ruber record document a short warm interval associated with a turnover from carnivorous to herbivorous-opportunistic planktonic foraminiferal species and an increase in abundance of calcareous nannofossil Florisphaera profunda. At ca. 1935 AD, d18O G. ruber data indicate a gradually warming, concomitant an evident turnover from carnivorous to herbivorous-opportunistic planktonic foraminiferal species, and associated to the increase of calcareous nannofossil F. profunda. From ca. 1950 AD to the present day the warm phase documents a further strong increase in G. ruber alba, G. quadrilobatus and F. profunda abundances. The pollen analysis carried out on the same core revealed a two-step increase in arboreal vegetation, this is mostly related to a rise of Olea, Quercus evergreen and riparian trees (Alnus and Salix) from ca. 1882 AD, and increase in conifers (Pinus and Cupressaceae) from ca. 1980 AD. The short lived warm interval documented by d18O between ca. 1865 and ca. 1882 AD may triggered the increase in Olea and the coeval general decrease in deciduous trees. Among herbs, Cyperaceae, along with ferns

  15. Morphologically uniform bats Hipposideros aff. ruber (Hipposideridae) exhibit high mitochondrial genetic diversity in southeastern Senegal

    Czech Academy of Sciences Publication Activity Database

    Vallo, Peter; Benda, P.; Martínková, Natália; Kaňuch, Peter; Kalko, E. K. V.; Červený, Jaroslav; Koubek, Petr

    2011-01-01

    Roč. 13, č. 1 (2011), s. 79-88 ISSN 1508-1109 R&D Projects: GA AV ČR IAA6093404 Institutional research plan: CEZ:AV0Z60930519 Keywords : cytochrome b * Hipposideros caffer complex * cryptic species * phylogeny Subject RIV: EG - Zoology Impact factor: 1.116, year: 2011

  16. Sugarcane bagasse hydrolysate as a potential feedstock for red pigment production by Monascus ruber

    DEFF Research Database (Denmark)

    Terán Hilares, Ruly; de Souza, Rebeca Andrade; Marcelino, Paulo Franco

    2018-01-01

    condition (7.45 UA490nm). By using SCB hydrolysate-based medium, the highest red pigment production (18.71 AU490nm) was achieved under dark condition and the glucose and cellobiose present in the hydrolysate were metabolized. SCB enzymatic hydrolysate was demonstrated to be a promising carbon source...

  17. Eesti kirjandus Moskvas : ajutine vaikus : [intervjuu] / Vera Ruber, Aleksandr Tomberg ; üles kirjut. Triin Parts

    Index Scriptorium Estoniae

    Ruber, Vera, 1925-2011

    1997-01-01

    Emil Tode (T. Õnnepalu) 'Piiririik' ilmub Vera Ruberi tõlkes ajakirja 'Druzhba Narodov' detsembrinumbris. Aleksandr Tombergil on valmis romaani 'Hind' tõlge, kuid seni puuduvad avaldamisvõimalused

  18. 'Szhigaja eti kulturnõje mostõ...' : Moskva-Tallinn 1958-1986 / Vera Ruber

    Index Scriptorium Estoniae

    Ruber, Vera, 1925-2011

    1997-01-01

    Tööst eesti kirjanduse konsultandina Üleliidulise Kirjanike Liidu juhatusse sekretariaadis. Ka 1959. a. romaanivõistlusel silmapaistnud romaanidest (Lilli Prometi 'Meesteta küla' ja Raimond Kaugveri 'Võõra mõõga teenistuses'), Lilli Prometi romaanist 'Primavera' ja venekeelsest kogumikust 'Eesti noor proosa' (1978)

  19. Seleção de linhagens de feijão rosinha de boa cocção, resistentes à antracnose e mancha angular Selection of pink grain common bean lines with good cooking ability, resistance to anthracnose and angular leaf spot

    Directory of Open Access Journals (Sweden)

    Diego Velásquez Faleiro e Silva

    2009-09-01

    Full Text Available Cultivares de feijoeiro com grão rosinha foram de grande importância, no passado, e ainda hoje, mesmo com a preferência pelo grão carioca, há nichos de mercado para feijões do grupo Rosinha. Dessa forma, o objetivo do trabalho foi selecionar linhagens de feijoeiro comum com grão rosinha, alta produção de grãos, rápido cozimento e resistentes à antracnose e à mancha angular. A partir de cinco famílias F8 superiores, provenientes do cruzamento entre os genitores Rosinha Maria da Fé e ESAL 693, foram extraídas 143 linhagens as quais foram avaliadas na safra das águas de 2005/2006 em Lavras (MG. Dessas, manteve-se 99 linhagens que foram avaliadas em Lavras e Lambari (MG, na safra da seca de 2006. As 24 linhagens selecionadas foram novamente avaliadas nos dois locais, no inverno de 2006. Os caracteres avaliados foram produção e tipo de grão, tempo de cocção, reação à mancha angular e também, foi realizado o teste de resistência ao patótipo 65 de Colletotrichum lindemuthianum. Nas linhagens, observaram-se variabilidade genética para todos os caracteres avaliados, altas estimativas dos coeficientes de herdabilidade, assegurando elevados ganhos com a seleção. Foram selecionadas quatro linhagens com alta produção, tipo de grão ideal, com rápido tempo de cocção e resistência à mancha angular e à antracnose.Common bean cultivars with pink grain type used to be very important, although there is still some market spot for them. The objective of the research was to select common bean lines with pink grain, high grain yield with good cooking ability, and resistance to anthracnose and angular leaf spot. One hundred and forty three lines were selected from five F8 segregant families derived from the cross Rosinha Maria da Fé x ESAL 693. Those lines were evaluated in the rainy season of 2005/2006 at Lavras county, MG State. Ninety nine lines were kept and tested in the dry season of 2006 at Lavras and Lambari. The 24

  20. Modelling planktic foraminifer growth and distribution using an ecophysiological multi-species approach

    Directory of Open Access Journals (Sweden)

    F. Lombard

    2011-04-01

    Full Text Available We present an eco-physiological model reproducing the growth of eight foraminifer species (Neogloboquadrina pachyderma, Neogloboquadrina incompta, Neogloboquadrina dutertrei, Globigerina bulloides, Globigerinoides ruber, Globigerinoides sacculifer, Globigerinella siphonifera and Orbulina universa. By using the main physiological rates of foraminifers (nutrition, respiration, symbiotic photosynthesis, this model estimates their growth as a function of temperature, light availability, and food concentration. Model parameters are directly derived or calibrated from experimental observations and only the influence of food concentration (estimated via Chlorophyll-a concentration was calibrated against field observations. Growth rates estimated from the model show positive correlation with observed abundance from plankton net data suggesting close coupling between individual growth and population abundance. This observation was used to directly estimate potential abundance from the model-derived growth. Using satellite data, the model simulate the dominant foraminifer species with a 70.5% efficiency when compared to a data set of 576 field observations worldwide. Using outputs of a biogeochemical model of the global ocean (PISCES instead of satellite images as forcing variables gives also good results, but with lower efficiency (58.9%. Compared to core tops observations, the model also correctly reproduces the relative worldwide abundance and the diversity of the eight species when using either satellite data either PISCES results. This model allows prediction of the season and water depth at which each species has its maximum abundance potential. This offers promising perspectives for both an improved quantification of paleoceanographic reconstructions and for a better understanding of the foraminiferal role in the marine carbon cycle.

  1. Annual change in the rate of participation in breast cancer screening through active encouragement of sports participation. A survey of women participants at the annual meetings of the Pink Ribbon Ladies' Tennis Tournament organized by the Japan Women's Tennis Players' League

    International Nuclear Information System (INIS)

    Shimada, Naoko; Nozue; Etsuko; Fukuda, Mamoru; Sawai, Kiyoshi; Kasumi, Fujio

    2007-01-01

    In March 2002, an initial attempt was made to decrease breast cancer mortality by the Japan Society of Breast Health, by means of encouraging participation in sports. This was followed by other similar events. The present study was designed to examine whether these kinds of sport-associated events are actually effective for increasing the screening participation rate. We hoped that the results would reveal practical ways of organizing such programs. One of these activities, the All Japan Women's Tennis Players' League, has called for amateur players to participate in an annual meeting of the Pink Ribbon Ladies' Tennis Tournament since 2003. A survey of their knowledge about breast cancer and their will to participate in breast cancer screening has been carried out annually in 2003, 2004 and 2005, by asking the participants to respond to our questionnaires. As a result, the number of participants has increased: from 7,201 women in 2003, to 7,846 in 2004 and to 8,572 in 2005. The questionnaires included items about participation in breast cancer screening, performance of self-examination, and participation in mammography screening. The participation rate increased year by year. The participation rate at mammography screening was 21% in 2003, and this increased to 26% in 2005. Thus this kind of sports event appears to promote knowledge about breast cancer screening and to increase the participation rate. On the other hand, it was found that the rate of self-examination decreased from 53% to 22%. Therefore problems that need to be addressed in the future include not only increasing women's motivation to undergo screening, but also the selection of appropriate screening methods, their combination, and distribution of accurate information. (author)

  2. Estimación de mortalidad natural e incertidumbre para congrio dorado (Genypterus blacodes Schneider, 1801 en la zona sur-austral de Chile Estimation of natural mortality and uncertainty in pink cusk-eel (Genypterus blacodes Schneider, 1801 in southern Chile

    Directory of Open Access Journals (Sweden)

    Rodrigo Wiff

    2011-07-01

    Full Text Available El congrio dorado (Genypterus blacodes es un pez demersal de gran importancia económica en la pesquería multiespecífica y multiflota que opera en la zona sur-austral de Chile (41°28'-57°00'S. Desde principio de los años noventas se consideró para efectos de la evaluación poblacional la existencia de un único stock. Sin embargo, varios antecedentes relacionados con la historia de vida y demografía han conducido a que, desde el año 2005, la evaluación de esta especie se realice bajo dos stocks administrativos, uno en la zona norte (41°28'-47°00'S y otro en la zona sur (47°00'-57°00'S. La separación de stocks produce una demanda por actualización de parámetros de historia de vida, entre éstos la mortalidad natural (M. En este trabajo se estimó M para el congrio dorado mediante métodos empíricos aplicados por zona y sexo. La incertidumbre en M fue incorporada a través de remuestreo de Monte Carlo considerando dos fuentes de error, una proveniente de los parámetros de historia de vida que alimentan los modelos empíricos y otra, proveniente de los coeficientes que los definen. El promedio de M mediante los diferentes métodos mostró, para una determinada zona, importantes diferencias entre sexos, como también para sexos conjuntos entre zonas de pesca. Los individuos de la zona norte presentaron mayor M que aquellos provenientes de la zona sur y los coeficientes de variación por método son altamente dependientes del tipo de error incorporado. El método de Pauly (1980 parece ser el más adecuado para el congrio dorado entregando valores de M para sexos conjuntos de 0,27 año-1 (IC: 0,13-0,47 en la zona norte y 0,23 año-1 (IC: 0,11-0,40 en la zona sur.Pink cusk-eel (Genypterus blacodes is a demersal fish of high economic importance for the multi-species and multi-fleet fishery operating off far-southern Chile (41°28'-57°00'S. Since the early 1990s, the existence of a single stock was assumed for purposes of stock

  3. Environmental controls on barium incorporation into planktic foraminifer, Globorotalia truncatulinoides

    Science.gov (United States)

    Richey, J. N.; Reynolds, C. E.; Fehrenbacher, J. S.

    2017-12-01

    The Ba/Ca of planktic foraminifera in marine sediment cores has been used primarily to track changes in riverine input over time, and thus may be a potentially powerful proxy for reconstructing past changes in the terrestrial hydrologic cycle. Using Ba/Ca as a proxy for riverine freshwater input requires the assumption that Ba/Ca in foraminiferal calcite reflects the Ba/Ca of seawater, and that the partition coefficient for barium between seawater and foraminiferal calcite is independent of the influence of temperature, salinity, pH, alkalinity and light availability. Although it has been shown that this partition coefficient is nearly identical for common species of spinose planktic foraminifera (e.g., Globigerinoides ruber, Orbulina universa, Globigerinoides sacculifer), some non-spinose species have been demonstrated to have Ba/Ca ratios that are much higher than that of co-occurring spinose specimens. We investigate environmental controls on Ba/Ca in the tests of Globorotalia truncatulinoides, a planktic species of foraminifera with a unique life history in the Gulf of Mexico (GoM). G. truncatulinoides experiences 92% of its annual flux to the sediment trap during winter (JFM) in the GoM. The Mg/Ca and ∂18O of the ontogenetic calcite suggests that primary calcification occurs within the surface mixed layer (0-150 meters), and a thick secondary crust is added at depths below the thermocline. We use LA-ICP-MS to analyze the Ba/Ca of both encrusted and non-encrusted G. truncatulinoides from a sediment trap time series in the GoM and find that the Ba/Ca in ontogenetic calcite of non-encrusted specimens varies between 10 and 200 mmol/mol, while the Ba/Ca of the secondary crust varies between 0 and 3 mmol/mol. The Ba/Ca of the non-encrusted G. truncatulinoides specimens is two to three orders of magnitude higher than that of co-occurring spinose planktic foraminifera (O. universa and G. ruber) in the GoM sediment trap, while the secondary crust has Ba/Ca similar to

  4. Low planktic foraminiferal diversity and abundance observed in a spring 2013 west-east Mediterranean Sea plankton tow transect

    Science.gov (United States)

    Mallo, Miguel; Ziveri, Patrizia; Mortyn, P. Graham; Schiebel, Ralf; Grelaud, Michael

    2017-05-01

    Planktic foraminifera were collected with 150 µm BONGO nets from the upper 200 m water depth at 20 stations across the Mediterranean Sea between 2 May and 2 June 2013. The main aim is to characterize the species distribution and test the covariance between foraminiferal area density (ρA) and seawater carbonate chemistry in a biogeochemical gradient including ultraoligotrophic conditions. Average foraminifera abundances are 1.42 ± 1.43 ind. 10 m-3 (ranging from 0.11 to 5.20 ind. 10 m-3), including 12 morphospecies. Large differences in species assemblages and total abundances are observed between the different Mediterranean sub-basins, with an overall dominance of spinose, symbiont-bearing species indicating oligotrophic conditions. The highest values in absolute abundance are found in the Strait of Gibraltar and the Alboran Sea. The western basin is dominated by Globorotalia inflata and Globigerina bulloides at slightly lower standing stocks than in the eastern basin. In contrast, the planktic foraminiferal assemblage in the warmer, saltier, and more nutrient-limited eastern basin is dominated by Globigerinoides ruber (white). These new results, when combined with previous findings, suggest that temperature-induced surface water stratification and food availability are the main factors controlling foraminiferal distribution. In the oligotrophic and highly alkaline and supersaturated with respect to calcite and aragonite Mediterranean surface water, standing stocks and ρA of G. ruber (white) and G. bulloides are affected by both food availability and seawater carbonate chemistry. Rapid warming increased surface ocean stratification impacting food availability and changes in trophic conditions could be the causes of reduced foraminiferal abundance, diversity, and species-specific changes in planktic foraminiferal calcification.

  5. HOW PINK ARE YOU? : Creating Awareness of the Gay Market

    OpenAIRE

    Orlik, Inez

    2012-01-01

    The objective of the thesis was to gather information on as well as draw attention to the Gay Market among representatives connected to the tourism business who met during the international fair Matka Expo 2012 held in the Helsinki Exposition and Convention Centre. The aim was to seek to improve the future situation on this particular niche market through increased interest and knowledge, which would lead to better understanding, cooperation and prejudice release. The thesis was aimed to make...

  6. The pink pigment prodigiosin: Vibrational spectroscopy and DFT calculations

    Czech Academy of Sciences Publication Activity Database

    Jehlička, J.; Němec, I.; Varnali, T.; Culka, A.; Svatoš, A.; Frank, Otakar; Oren, A.; Edwards, G.M.

    2016-01-01

    Roč. 134, NOV 2016 (2016), s. 234-243 ISSN 0143-7208 Institutional support: RVO:61388955 Keywords : prodigiosin * serratia marcescens * raman spectroscopy Subject RIV: CG - Electrochemistry Impact factor: 3.473, year: 2016

  7. Planet Mercury from pale pink dot to dynamic world

    CERN Document Server

    Rothery, David A

    2014-01-01

    A new and detailed picture of Mercury is emerging thanks to NASA's MESSENGER mission that spent four years in orbit about the Sun's innermost planet. Comprehensively illustrated by close-up images and other data, the author describes Mercury's landscapes from a geological perspective: from sublimation hollows, to volcanic vents, to lava plains, to giant thrust faults. He considers what its giant core, internal structure and weird composition have to tell us about the formation and evolution of a planet so close to the Sun. This is of special significance in view of the discovery of so many ex

  8. Pink Card: Tax Issues Affecting International Students, Faculty, and Staff.

    Science.gov (United States)

    Somers, Patricia; And Others

    1993-01-01

    The Internal Revenue Service and Immigration and Naturalization Service have increased monitoring of tax withholding for international scholars. Higher education institutions and scholars alike will benefit from a thorough understanding of tax treaties, nonresident alien status, income taxation, and social security tax obligations and periodic…

  9. Using functional genomics to study PINK1 and metabolic physiology

    DEFF Research Database (Denmark)

    Scheele, Camilla; Larsson, Ola; Timmons, James A

    2009-01-01

    Genome sequencing projects have provided the substrate for an unimaginable number of biological experiments. Further, genomic technologies such as microarrays and quantitative and exquisitely sensitive techniques such as real-time quantitative polymerase chain reaction have made it possible to re...... to be simpler than the in vivo mammalian tissue and thus the methods discussed largely apply to this cell biology phase. We apologize for not referring to all relevant publications and for any technical considerations we have also failed to factor into our discussion....

  10. Pink-Beam, Highly-Accurate Compact Water Cooled Slits

    International Nuclear Information System (INIS)

    Lyndaker, Aaron; Deyhim, Alex; Jayne, Richard; Waterman, Dave; Caletka, Dave; Steadman, Paul; Dhesi, Sarnjeet

    2007-01-01

    Advanced Design Consulting, Inc. (ADC) has designed accurate compact slits for applications where high precision is required. The system consists of vertical and horizontal slit mechanisms, a vacuum vessel which houses them, water cooling lines with vacuum guards connected to the individual blades, stepper motors with linear encoders, limit (home position) switches and electrical connections including internal wiring for a drain current measurement system. The total slit size is adjustable from 0 to 15 mm both vertically and horizontally. Each of the four blades are individually controlled and motorized. In this paper, a summary of the design and Finite Element Analysis of the system are presented

  11. Pink truck ads: second-wave feminism and gendered marketing.

    Science.gov (United States)

    Howard, Ella

    2010-01-01

    Second-wave feminist media had a contentious relationship with corporate advertisers. This article uses automotive advertisements to explore the role of gender, class, and race in the construction of consumer markets from the 1970s through the 1980s. It analyzes the struggle of Gloria Steinem and other liberal feminists to navigate the terrain between the women's movement and corporate advertisers. The increased economic power of women, stemming from the Equal Credit Opportunity Act as well as broader social and political shifts, facilitated their efforts. In the 1980s, automobiles continued to be marketed to women, albeit through "feminine" imagery conforming to the era's dominant trends.

  12. Pink Masks: Obstructive Sleep Apnoea and the Sociology of Diagnosis

    Directory of Open Access Journals (Sweden)

    SHARON HANCOCK

    2013-01-01

    Full Text Available The sociology of diagnosis takes a new look at diagnostic categories, the means by which they are delivered, and the social consequences of diagnosis for patient and professional alike. Considering the social elements which contribute to the recognition of disease categories and their consequences highlights important phenomena which can enrich the thinking of nurses regardless of whether or not they diagnose as part of their practice. However, the principles of the sociology of diagnosis, while widely debated in academe, have yet to penetrate nursing literature. In this discussion article, we use obstructive sleep apnoea in women as an exemplar to illustrate how a clearly material, pathophysiological disorder has, nonetheless, significant social “content.” We demonstrate the social structures and interests which shape obstructive sleep apnoea as a male disease, and the risks, paradoxically, of both under- and over-diagnosis that arise from this social construction. We use this example to exhort nurses to consider how the social and the biological intermesh and shape how we perceive disease and its impact. This should open the door for more responsive and responsible health care.

  13. "PINK1"-Linked Parkinsonism Is Associated with Lewy Body Pathology

    Science.gov (United States)

    Samaranch, Lluis; Lorenzo-Betancor, Oswaldo; Arbelo, Jose M.; Ferrer, Isidre; Lorenzo, Elena; Irigoyen, Jaione; Pastor, Maria A.; Marrero, Carmen; Isla, Concepcion; Herrera-Henriquez, Joanna; Pastor, Pau

    2010-01-01

    Phosphatase and tensin homolog-induced putative kinase 1 gene mutations have been associated with autosomal recessive early-onset Parkinson's disease. To date, no neuropathological reports have been published from patients with Parkinson's disease with both phosphatase and tensin homolog-induced putative kinase 1 gene copies mutated. We analysed…

  14. Home range of Noack's round-leaf bat (Hipposideros aff. ruber) in an agricultural landscape of central Ghana

    Czech Academy of Sciences Publication Activity Database

    Nkrumah, E. E.; Vallo, Peter; Klose, S. M.; Ripperger, S.; Badu, E. K.; Drosten, C.; Kalko, E. K. V.; Tschapka, M.; Oppong, S. K.

    2016-01-01

    Roč. 18, č. 1 (2016), s. 239-247 ISSN 1508-1109 Institutional support: RVO:68081766 Keywords : core area * foraging area * local convex hull * maximum foraging distance * radio-tracking Subject RIV: EG - Zoology Impact factor: 1.040, year: 2016

  15. Foraging behavior and habitat selection of Noack’s round-leaf bat (Hipposideros aff. ruber) and conservation implications

    Czech Academy of Sciences Publication Activity Database

    Nkrumah, E. E.; Vallo, Peter; Klose, S. M.; Ripperger, S.; Badu, E. K.; Gloza-Rausch, F.; Drosten, C.; Kalko, E. K. V.; Tschapka, M.; Oppong, S. K.

    2016-01-01

    Roč. 9, č. 4 (2016), s. 1-11 ISSN 1940-0829 Institutional support: RVO:68081766 Keywords : agro-environment * cocoa farms * fallow lands * seminatural habitats * sub-Saharan Africa Subject RIV: EG - Zoology Impact factor: 1.238, year: 2016

  16. Molecular and biochemical characterization of a new thermostable bacterial laccase from Meiothermus ruber DSM 1279

    DEFF Research Database (Denmark)

    Kalyani, D. C.; Munk, L.; Mikkelsen, J. D.

    2016-01-01

    . Spectroscopic analysis of the purified enzyme by UV/visible and electron paramagnetic resonance spectroscopy confirmed that the Mrlac was a multicopper oxidase. The Mrlac had a molecular weight of ∼ 50 kDa and exhibited activity towards the canonical laccase substrates 2,2'-azino-bis(3-ethylbenzothiazoline-6...

  17. Size-dependent response of foraminiferal calcification to seawater carbonate chemistry

    Science.gov (United States)

    Henehan, Michael J.; Evans, David; Shankle, Madison; Burke, Janet E.; Foster, Gavin L.; Anagnostou, Eleni; Chalk, Thomas B.; Stewart, Joseph A.; Alt, Claudia H. S.; Durrant, Joseph; Hull, Pincelli M.

    2017-07-01

    The response of the marine carbon cycle to changes in atmospheric CO2 concentrations will be determined, in part, by the relative response of calcifying and non-calcifying organisms to global change. Planktonic foraminifera are responsible for a quarter or more of global carbonate production, therefore understanding the sensitivity of calcification in these organisms to environmental change is critical. Despite this, there remains little consensus as to whether, or to what extent, chemical and physical factors affect foraminiferal calcification. To address this, we directly test the effect of multiple controls on calcification in culture experiments and core-top measurements of Globigerinoides ruber. We find that two factors, body size and the carbonate system, strongly influence calcification intensity in life, but that exposure to corrosive bottom waters can overprint this signal post mortem. Using a simple model for the addition of calcite through ontogeny, we show that variable body size between and within datasets could complicate studies that examine environmental controls on foraminiferal shell weight. In addition, we suggest that size could ultimately play a role in determining whether calcification will increase or decrease with acidification. Our models highlight that knowledge of the specific morphological and physiological mechanisms driving ontogenetic change in calcification in different species will be critical in predicting the response of foraminiferal calcification to future change in atmospheric pCO2.

  18. The response of calcifying plankton to climate change in the Pliocene

    Directory of Open Access Journals (Sweden)

    C. V. Davis

    2013-09-01

    Full Text Available As a result of anthropogenic pCO2 increases, future oceans are growing warmer and lower in pH and oxygen, conditions that are likely to impact planktic communities. Past intervals of elevated and changing pCO2 and temperatures can offer a glimpse into the response of marine calcifying plankton to changes in surface oceans under conditions similar to those projected for the future. Here we present new records of planktic foraminiferal and coccolith calcification (weight and size from Deep Sea Drilling Project Site 607 (mid-North Atlantic and Ocean Drilling Program Site 999 (Caribbean Sea from the Pliocene, the last time that pCO2 was similar to today, and extending through a global cooling event into the intensification of Northern Hemisphere glaciation (3.3 to 2.6 million years ago. Test weights of both surface-dwelling Foraminifera Globigerina bulloides and thermocline-dwelling Foraminifera Globorotalia puncticulata vary with a potential link to regional temperature variation in the North Atlantic, whereas in the tropics Globigerinoides ruber test weight remains stable. In contrast, reticulofenestrid coccoliths show a narrowing size range and a decline in the largest lith diameters over this interval. Our results suggest no major changes in plankton calcite production during the high pCO2 Pliocene or during the transition into an icehouse world.

  19. Orbital-scale denitrification changes in the Eastern Arabian Sea during the last 800 kyrs.

    Science.gov (United States)

    Kim, Ji-Eun; Khim, Boo-Keun; Ikehara, Minoru; Lee, Jongmin

    2018-05-04

    Denitrification in the Arabian Sea is closely related to the monsoon-induced upwelling and subsequent phytoplankton production in the surface water. The δ 15 N values of bulk sediments collected at Site U1456 of the International Ocean Discovery Program (IODP) Expedition 355 reveal the orbital-scale denitrification history in response to the Indian Monsoon. Age reconstruction based on the correlation of planktonic foraminifera (Globigerinoides ruber) δ 18 O values with the LR04 stack together with the shipboard biostratigraphic and paleomagnetic data assigns the study interval to be 1.2 Ma. Comparison of δ 15 N values during the last 800 kyrs between Site U1456 (Eastern Arabian Sea) and Site 722B (Western Arabian Sea) showed that δ 15 N values were high during interglacial periods, indicating intensified denitrification, while the opposite was observed during glacial periods. Taking 6‰ as the empirical threshold of denitrification, the Eastern Arabian Sea has experienced a persistent oxygen minimum zone (OMZ) to maintain strong denitrification whereas the Western Arabian Sea has undergone OMZ breakdown during some glacial periods. The results of this study also suggests that five principal oceanographic conditions were changed in response to the Indian Monsoon following the interglacial and glacial cycles, which controls the degree of denitrification in the Arabian Sea.

  20. Holocene aridification of India

    Science.gov (United States)

    Ponton, C.; Giosan, L.; Eglinton, T.I.; Fuller, D.Q.; Johnson, J.E.; Kumar, P.; Collett, T.S.

    2012-01-01

    Spanning a latitudinal range typical for deserts, the Indian peninsula is fertile instead and sustains over a billion people through monsoonal rains. Despite the strong link between climate and society, our knowledge of the long-term monsoon variability is incomplete over the Indian subcontinent. Here we reconstruct the Holocene paleoclimate in the core monsoon zone (CMZ) of the Indian peninsula using a sediment core recovered offshore from the mouth of Godavari River. Carbon isotopes of sedimentary leaf waxes provide an integrated and regionally extensive record of the flora in the CMZ and document a gradual increase in aridity-adapted vegetation from ???4,000 until 1,700 years ago followed by the persistence of aridity-adapted plants after that. The oxygen isotopic composition of planktonic foraminifer Globigerinoides ruber detects unprecedented high salinity events in the Bay of Bengal over the last 3,000 years, and especially after 1,700 years ago, which suggest that the CMZ aridification intensified in the late Holocene through a series of sub-millennial dry episodes. Cultural changes occurred across the Indian subcontinent as the climate became more arid after ???4,000 years. Sedentary agriculture took hold in the drying central and south India, while the urban Harappan civilization collapsed in the already arid Indus basin. The establishment of a more variable hydroclimate over the last ca. 1,700 years may have led to the rapid proliferation of water-conservation technology in south India. Copyright 2012 by the American Geophysical Union.

  1. Métodos de extrusão manual e elétrica dos espermat��foros de reprodutores selvagens do camarão-rosa Farfantepenaeus paulensis (Decapoda: Penaeidae Manual and electrical spermatophore extrusion methods of the pink shrimp Farfantepenaeus paulensis (Decapoda: Penaeidae wild broodstock

    Directory of Open Access Journals (Sweden)

    Cintia Nakayama

    2008-10-01

    Full Text Available O presente estudo comparou os métodos manual e elétrico de extrusão do espermatóforo do camarão-rosa Farfantepenaeus paulensis com o objetivo de verificar se os métodos de extrusão exercem influência na quantidade de células espermáticas e na regeneração de novos espermatóforos. Os machos foram extrusados no início (dia zero e no final do experimento (dia 43 para verificação da eficiência dos métodos no processo de regeneração. A extrusão manual foi realizada por meio de pressão na região do quinto par de pereiópodos e o método elétrico com uso de eletrodo para transmissão de impulso elétrico de 9 volts na mesma região. Os dois métodos foram considerados eficientes, não sendo encontradas diferenças significativas entre estes (P>0,05 para o número de células espermáticas. Entretanto, foi verificada, no final do experimento, a perda de peso corporal, peso de espermatóforo e menor índice espermatossomático (IES (PThe present study compared manual and electrical methods to extrude the spermatophore of the pink shrimp Farfantepenaeus paulensis aiming to analyze their influence on the number of spermatic cells and spermatophore regeneration. The males were extruded in the beginning (day zero and in the end (43rd day of the experiment to evaluate the efficiency of these methods in the regeneration process. For the extrusion, a gentle pressure was applied manually in the fifth pair of pereiopods or electrically by a 9 volt pulse in the same area. Both methods were efficient in removing the spermatophore and no significant differences were found (P>0.05 in the number of sperm cells. Nevertheless, significant decreases (P<0.05 in the body weight, spermatophore weight and spermatosomatic index (ESI at the end of the experimental period were observed by using the electrical stimulation. The mean values (±SD of the number of sperm cells were 1.46 (±0.84 and 3.25 (±2.12 millions for the electrical and manual treatments

  2. Spatio-temporal variation of the pink shrimp Farfantepenaeus paulensis (Crustacea, Decapoda, Penaeidae associated to the seasonal overture of the sandbar in a subtropical lagoon Variação espaço-temporal do camarão rosa Farfantepenaeus paulensis (Crustacea, Decapoda, Penaeidae associado à abertura sazonal da barra de conexão com o mar em uma lagoa subtropical

    Directory of Open Access Journals (Sweden)

    Nicolle C Ferreira

    2009-12-01

    Full Text Available On the southern Brazilian coast, the penaeid pink shrimp Farfantepenaeus paulensis (Pérez-Farfante, 1967 is intensively captured by both artisanal and industrial fisheries. In the Ibiraquera Lagoon, artisanal fishing of juvenile stocks has declined sharply over the last 15 years. The traditional management methods used by fishermen are no longer sustainable due to conflicts with the demands of tourism and weak environmental and public agencies. The dynamics of the timing of the artificial opening and natural reformation of a sandbar across the mouth of the lagoon are regarded as a central socio-ecological problem. We evaluated the abundance and biomass variation of F. paulensis throughout the year and along a spatial gradient measured from the sandbar into the lagoon. We also explored the influence of opening the sandbar on the shrimp population. Samples were collected monthly from February 2004 to February 2005, using a 27 mm mesh cast net, in four different areas, with three sites being sampled per area. Small numbers of large shrimps were captured in the upper area, while greater biomass and abundance of small shrimps were observed near the sandbar, suggesting the role of the inner area as a refuge for growing. The highest records for biomass and abundance were recorded during warm months, regardless if the lagoon was open or closed by the sandbar. The Ibiraquera Lagoon could be more productive for fishermen if the sandbar opening was coincident to the period of natural post-larvae influx. Furthermore, coastal stocks could be improved by opening the sandbar again during summer in conjunction with fishing quotas.O camarão rosa Farfantepenaeus paulensis (Pérez-Farfante, 1967 é intensivamente capturado, tanto pela frota pesqueira industrial quanto artesanal na costa sul brasileira. Na Lagoa de Ibiraquera (SC, a captura artesanal de camarões tem diminuído gradativamente nos últimos 15 anos. Os métodos de gestão tradicionais utilizados

  3. Microbial growth on oxalate by a route not involving glyoxylate carboligase

    Science.gov (United States)

    Blackmore, Maureen A.; Quayle, J. R.

    1970-01-01

    1. The metabolism of oxalate by the pink-pigmented organisms, Pseudomonas AM1, Pseudomonas AM2, Protaminobacter ruber and Pseudomonas extorquens has been compared with that of the non-pigmented Pseudomonas oxalaticus. 2. During growth on oxalate, all the organisms contain oxalyl-CoA decarboxylase, formate dehydrogenase and oxalyl-CoA reductase. This is consistent with oxidation of oxalate to carbon dioxide taking place via oxalyl-CoA, formyl-CoA and formate as intermediates, and also reduction of oxalate to glyoxylate taking place via oxalyl-CoA. 3. The pink-pigmented organisms, when grown on oxalate, contain l-serine–glyoxylate aminotransferase and hydroxypyruvate reductase but do not contain glyoxylate carboligase. The converse of this obtains in oxalate-grown Ps. oxalaticus. This indicates that, in contrast with Ps. oxalaticus, synthesis of C3 compounds from oxalate by the pink-pigmented organisms occurs by a variant of the `serine pathway' used by Pseudomonas AM1 during growth on C1 compounds. 4. Evidence in favour of this scheme is provided by the finding that a mutant of Pseudomonas AM1 that lacks hydroxypyruvate reductase is not able to grow on oxalate. PMID:5472155

  4. Evaluating controls on planktonic foraminiferal geochemistry in the Eastern Tropical North Pacific

    Science.gov (United States)

    Gibson, Kelly Ann; Thunell, Robert C.; Machain-Castillo, Maria Luisa; Fehrenbacher, Jennifer; Spero, Howard J.; Wejnert, Kate; Nava-Fernández, Xinantecatl; Tappa, Eric J.

    2016-10-01

    To explore relationships between water column hydrography and foraminiferal geochemistry in the Eastern Tropical North Pacific, we present δ18O and Mg/Ca records from three species of planktonic foraminifera, Globigerinoides ruber, Globigerina bulloides, and Globorotalia menardii, collected from a sediment trap mooring maintained in the Gulf of Tehuantepec from 2006-2012. Differences in δ18O between mixed-layer species G. ruber and G. bulloides and thermocline-dweller G. menardii track seasonal changes in upwelling. The records suggest an increase in upwelling during the peak positive phase of El Niño, and an overall reduction in stratification over the six-year period. For all three species, Mg/Ca ratios are higher than what has been reported in previous studies, and show poor correlations to calcification temperature. We suggest that low pH (7.6-8.0) and [3 2-CO] values (∼70-120 μmol/kg) in the mixed layer contribute to an overall trend of higher Mg/Ca ratios in this region. Laser Ablation Inductively Coupled Mass Spectrometry analyses of G. bulloides with high Mg/Ca ratios (>9 mmol/mol) reveal the presence of a secondary coating of inorganic calcite that has Mg/Ca and Mn/Ca ratios up to an order of magnitude higher than these elemental ratios in the primary calcite, along with elevated Sr/Ca and Ba/Ca ratios. Some of the samples with abnormally high Mg/Ca are found during periods of high primary productivity, suggesting the alteration may be related to changes in carbonate saturation resulting from remineralization of organic matter in oxygen-poor waters in the water column. Although similar shell layering has been observed on fossil foraminifera, this is the first time such alteration has been studied in shells collected from the water column. Our results suggest a role for seawater carbonate chemistry in influencing foraminiferal calcite trace element:calcium ratios prior to deposition on the seafloor, particularly in high-productivity, low

  5. Millennial-Scale Planktic Foraminifer Faunal Variability in the East China Sea during the Past 40000 Years (IMAGES MD012404 from the Okinawa Trough

    Directory of Open Access Journals (Sweden)

    Yuan-Pin Chang

    2008-01-01

    Full Text Available High resolution planktic foraminifer fauna assemblage data are used to reconstruct the millennial-scale sea surface temperature (SST variability of the past 40000 years at an IMAGES core site (MD012404 in the Okinawa Trough in the East China Sea (ECS. The fauna assemblages in core MD012404 are dominated by five species - Globigerinoides ruber, Globigerina bulloides, Neogloboquadrina dutertrei, Pulleniatina obliquiloculata, and Globigerinita glutinata, which account for > 70% in relative abundance. Our Q-mode factor analysis decomposed the fauna abundance data into three factors, which indicate cold water mass, warm water mass, and possibly coastal water flow with low salinity in the ECS. The MD012404 fauna data show abrupt changes at ~16 kya, suggesting a return to a warmer climate or warm water intrusion of the Kuroshio into the Okinawa Trough since the Last Glacial Maximum (LGM. SST estimates based on the fauna assemblages of planktic foraminifers indicate a LGM cooling of 1 - 2°C. Amaximum cooling by 3 - 4°C is observed in episodic, millennial-scale events in the glacial stages of the record. The SST record displays variability that closely tracks the structure of oxygen isotopes of stalagmites from Hulu Cave and ice cores from GISP 2 Dansgaard/Oeschger cycles and Heinrich events. Low salinity in the ECS is inferred based on MD012404 fauna SST and planktic foraminifer oxygen isotope records for the cold millennial-scale intervals, pointing to the Intertropical Convergence Zone (ITCZ and/or East Asian monsoon as important factors driving SST and salinity in the subtropical western Pacific, both on orbital and suborbital time scales.

  6. Deglacial Western Equatorial Pacific pCO2 Reconstruction Using Boron Isotopes of Planktonic Foraminiferas

    Science.gov (United States)

    Kubota, K.; Yokoyama, Y.; Ishikawa, T.; Sagawa, T.; Ikehara, M.; Yamazaki, T.

    2017-12-01

    During the last deglaciation (ca. 19 - 11 ka), partial pressure of CO2 (pCO2) of the atmosphere increased by 80 μatm. Many paleoceanographers point out that the ocean had played an important role in atmospheric CO2 rise, since the ocean have 60 times larger capacity to store carbon compared to the atmosphere. However, evidence on where carbon was transferred from the ocean to the atmosphere is still lacking, hampering our understanding of global carbon cycles in glacial-interglacial timescales. Boron isotope of skeletons of marine calcifying organisms such as corals and foraminiferas can pin down where CO2 source/sink existed, because boron isotopes of marine calcium carbonates is dependent on seawater pH, from which pCO2 of the past seawater can be reconstructed. In previous studies using the boron isotope teqnique, Martinez-Boti et al. (2015, Nature) and Kubota et al. (2014, Scientific Reports) revealed that central and eastern parts of the equatorial Pacific acted as a CO2 source (i.e., CO2 emission) during the last deglaciation, suggesting the equatorial Pacific's contribution to atmospheric CO2 rise. However, some conflicting results have been confirmed in a marine sediment record from the western part of the equatorial Pacific (Palmer & Pearson, 2003, Science), making the conclusion elusive. In this presentation, we will show new results of Mg/Ca, oxygen isotope, and boron isotope measurements during the last 35 ka on two species of surface dwelling foraminiferas (Globigerinoides ruber and G. sacculifer) which was hand-picked separatedly from a well-dated marine sediment core recovered from the West Caroline Basin (KR05-15 PC01) (Yamazaki et al., 2008, GRL). From the new records, we will discuss how the equatorial Pacific behaved during the last deglaciation and how it related to the global carbon cycles.

  7. Indian Monsoon and denitrification change in the Laxmi Basin (IODP Exp. 355 Site U1456) of the Eastern Arabian Sea during the last 800 kyrs

    Science.gov (United States)

    Kim, J. E.; Khim, B. K.; Ikehara, M.; Lee, J.

    2017-12-01

    The Arabian Sea is a famous site for the basin-wide denitrification in the globe. The Western Arabian Sea has been acknowledged by its upwelling-induced denitrification related to the Indian Monsoon system (Altabet et al., 1999). It was recently reported that the denitrification in the Eastern Arabian Sea (IODP Exp. 355 Site U1456) has been persistent and consistent during the mid-Pleistocene as reflected in the bulk sediment δ15N values (Tripathi et al., 2017). Based on the age model reconstructed by δ18O stratigraphy of planktonic foraminifera (Globigerinoides ruber) together with shipboard biostratigraphic and paleomagnetic data at Site U1456 drilled in the Laxmi Basin of the Eastern Arabian Sea, the glacial-interglacial fluctuations of denitrification in association with the development of oxygen minimum zone (OMZ) were resolved in the context of Indian Monsoon activity. One of striking features in the Eastern Arabian Sea is that the δ15N values of bulk sediment show clear and consistent denitrification with minimum δ15N values exceeding 6‰ even during glacial periods, when its western counterpart experienced a temporal collapse of OMZ and denitrification. The Eastern Arabian Sea is fed not only by the upwelling-induced productivity in the western margin during the summer monsoon but also by the high productivity during the winter monsoon, both of which maintain the increased productivity affecting the OMZ through the consumption of dissolved oxygen by the degradation of sinking organic particles. The Eastern Arabian Sea is further influenced by the clockwise surface currents, intermediate water ventilation change by the blockage of Antarctic Intermediate Water, limited inflow from the Red Sea/Persian Gulf, and the freshwater salinity stratification due to nearby riverine discharges, all of which make the denitrification process more complicated than the Western Arabian Sea. Nonetheless, the glacial-interglacial denitrification change in the Eastern

  8. Pleistocene Thermocline Reconstruction and Oxygen Minimum Zone Evolution in the Maldives

    Science.gov (United States)

    Yu, S. M.; Wright, J.

    2017-12-01

    Drift deposits of the southern flank the Kardiva Channel in the eastern Inner Sea of the Maldives provide a complete record of Pleistocene water column changes in conjunction with monsoon cyclicity and fluctuations in the current system. We sampled IODP Site 359-U1467 to reconstruct water column using foraminiferal stable isotope records. This unlithified lithostratigraphic unit is rich in well-preserved microfossils and has an average sedimentation rate of 3.4 cm/yr. Marine Isotope Stages 1-6 were identified and show higher sedimentation rates during the interglacial sections approaching 6 cm/kyr. We present the δ13C and δ18O record of planktonic and benthic foraminiferal species taken at intervals of 3 cm. Globigerinoides ruber was used to constrain surface conditions. The thermocline dwelling species, Globorotalia menardii, was chosen to monitor fluctuations in the thermocline compared to the mixed layer. Lastly, the δ13C of the benthic species, Cibicidoides subhaidingerii and Planulina renzi, reveal changes to the bottom water ventilation and expansion of oxygen minimum zones over time. All three taxa recorded similar changes in δ18O over the glacial/interglacial cycles which is remarkable given the large sea level change ( 120 m) and the relatively shallow water depth ( 450 m). There is a small increase in the δ13C gradient during the glacial intervals which might reflect less ventilated bottom waters in the Inner Sea. This multispecies approach allows us to better constrain the thermocline hydrography and suggests that changes in the OMZ thickness are driven by the intensification of the monsoon cycles while painting a more cohesive picture to the changes in the water column structure.

  9. Planktonic foraminiferal abnormalities in coastal and open marine eastern Mediterranean environments: A natural stress monitoring approach in recent and early Holocene marine systems

    Science.gov (United States)

    Antonarakou, A.; Kontakiotis, G.; Zarkogiannis, S.; Mortyn, P. G.; Drinia, H.; Koskeridou, E.; Anastasakis, G.

    2018-05-01

    Marine environmental status can be assessed through the study of bio-indicator species. Here, we monitor natural environmental stress by the occurrence of morphologically abnormal planktonic foraminiferal specimens from a suite of surface sediments in the eastern Mediterranean Sea. We also compare Scanning Electron Microscopy (SEM) abnormality observations from sapropel S1-derived sediments in the Aegean, Libyan and Levantine basins, since they provide a direct record of a natural stress experiment that took place over past time scales. At initial sapropel deposition levels, we observe increased growth asymmetry in Globigerinoides ruber twinned and twisted individuals, possibly associated with eutrophication and anoxia. In modern material, a range of malformations and aberrant morphologies from slight deformity with smaller or overdeveloped chambers to more severe deformity with abnormally protruding or misplaced chambers, distorted spirals, and double tests is also observed, as a result of the hypersaline, oligotrophic and oxygen-depleted nature of the Mediterranean Sea water column. Overall, we highlight the current use of the relative abundance of abnormal tests as a bio-indicator for monitoring natural stress, especially the occurrence of twin specimens as indicative of high-salinity stress conditions, and further illustrate the necessity to map both their spatial and temporal distribution for accurate paleoenvironmental reconstructions. Such an approach presents the advantage to rapidly provide information over wide spatial and temporal scales, extending our ability to monitor a wide variety of environments (from coastal to the open-sea). However, further investigations should extend this approach to test the robustness of our findings in a number of similar oceanic settings.

  10. Changes in Eastern Equatorial Pacific Thermocline Structure across the Last Deglaciation: Evidence from the Carnegie Ridge

    Science.gov (United States)

    Glaubke, R.; Schmidt, M. W.; Warner, L.; Hertzberg, J. E.; Marcantonio, F.; Bianchi, T. S.

    2017-12-01

    The eastern equatorial Pacific (EEP) is an important climatological region given its influence in the modulation of the El Niño - Southern Oscillation (ENSO). The current climatic mean state of the EEP is characterized by cool sea surface temperatures (SST) and a strong, shallow thermocline. Nevertheless, there remains significant uncertainty about past changes in tropical Pacific climate and how ENSO variability relates to the millennial-scale climate events of the last deglaciation. Here, we will present 21 kyrs of Mg/Ca paleotemperature data from the surface-dwelling foraminifera Globigerinoides ruber and the thermocline-dwelling foraminifera Neogloboquadrina dutertrei collected from piston core MV1014-02-17JC (00° 10.83'S, 85° 52.00'W; 2846 m depth) on the Carnegie Ridge. Initial results reveal a 1.3°C warming of the surface ocean from the early-Holocene until 6 kyrs, a trend present in other EEP SST reconstructions (Pena et al., 2008; Timmerman et al., 2014; Lea et al., 2000). The surface ocean subsequently cools from 6 kyrs and reaches present-day temperatures by 3.5 kyrs. The subsurface reveals a nearly monotonic cooling of 1.8°C from 10.8 kyrs to the present day, which suggest a gradual shoaling of the thermocline across the Holocene. Furthermore, an increase in the vertical temperature gradient occurs from the late- to mid-Holocene, with the sharpest temperature difference centered at 6 kyrs, coincident with the mid-Holocene peak in SSTs. Taken together, these data suggest a gradual shoaling of the thermocline across the Holocene, with the variations in SST primarily governing the intensity of the vertical temperature gradient. Future work includes extending this record back to the last glacial maximum (LGM) to assess tropical Pacific mean state change across the abrupt climate events that characterized the last deglaciation.

  11. AMS 14 C dating controlled records of monsoon and Indonesian throughflow variability from the eastern Indian Ocean of the past 32,000 years

    Science.gov (United States)

    Li, Z. Y.; Chen, M. T.; Shi, X.; Liu, S.; Wang, H.

    2015-12-01

    Zi-Ye Li a, Min-Te Chen b, Hou-Jie Wang a, Sheng-Fa Liu c, Xue-Fa Shi ca College of Marine Geosciences, Ocean University of China, Qingdao 266100, P.R. Chinab Institute of Applied Geosciences, National Taiwan Ocean University, Keelung, Taiwan 20224, ROCc First Institute of Oceanography, SOA, Qingdao 266100, P.R. China Indonesian throughflow (ITF) is one of the most important currents responsible for transporting heat and moisture from the western Pacific to the Indian Oceans. The ITF is also well-known as effectively in modulating the global climate change with the interactions among ENSO and Asian monsoons. Here we present an AMS 14C dating controlled sea surface temperature (SST) record from core SO184-10043 (07°18.57'S, 105°03.53'E), which was retrieved from 2171m water depth at a north-south depression located at the southeastern offshore area of Sumatera in the eastern Indian Ocean. Based on our high-resolution SST using Mg/Ca analyses based on planktonic foraminifera shells of Globigerinoides ruber and alkenone index, U k'37-SST, oxygen isotope stratigraphy, and AMC 14C age-controls, our records show that, during the past 32,000 years, the SSTs were decreased which imply weaker ITF during Marine Isotope Stage (MIS) 2 and 3. The weaker UTF may respond to strengthened northeast monsoon during the boreal winter. During 21 to 15ka, the southeast monsoon had been stronger and the northeast monsoon was relatively weaker. During 15 to 8ka, rapid sea level rising may allow the opening of the gateways in the Makassar Strait and Lombok Strait that may have further strengthened the ITF. During the early Holocene, the northeast and southeast monsoons seem to be both strengthened. We will discuss the implications of the hydrographic variability and their age uncertainties in this paper during the meeting.

  12. Late Quaternary Palaeoceanographic Changes in Sea Surface Conditions in the Tropical Atlantic

    Science.gov (United States)

    Fischel, Andrea; Seidenkrantz, Marit-Solveig; Kuijpers, Antoon; Nürnberg, Dirk

    2013-04-01

    Palaeoceanographic changes and the variability in surface water mass hydrography are reconstructed in order to track tropical ocean and climate variability and inter-hemispheric heat exchange through the last 42,000 year BP. Our studies are based on the relative abundance of planktonic foraminifera combined with sea surface temperature approximation based Mg/Ca measurements, XRF scanning and stable oxygen isotope analyses in a 5 m long gravity core Ga307-Win-12GC (17°50.80N, 64°48.7290W), retrieved in the Virgin Island Basin in approx. 3,960 m water depth. The Virgin Island Basin is the deepest part of the Anegada-Jungfern Passage in the northeast Caribbean, one of the most important pathways for water mass exchange between the Central Atlantic and the Caribbean Sea. Due to its bathymetry surface waters as well as deep water mass strata from the northern and southern hemisphere enter the basin, comprising Caribbean Surface Water (CSW), Antarctic Intermediate Water (AAIW), Atlantic Intermediate Water (AIW) and North Atlantic Deep Water (NADW). The planktonic foraminiferal assemblage suggests rather stable sea-surface conditions during the Holocene in the NE Caribbean. However, major changes in the hydrographic setting could be identified within the glacial period. During the glacial period, clear millennial-scale variability in sea-surface temperature and productivity are present. Fluctuations in the relative abundance of Globigerinoides ruber in the sediment core may be correlated to Dansgaard-Oeschger events in the northern North Atlantic. Furthermore an increase in relative abundance of Globorotalia rubescens occurs synchronous with ice rafted debris layers described from the North Atlantic. The faunal changes in the tropical Atlantic may thus be correlated to major climate changes in the North Atlantic, mainly D-O cyclicity as well as Heinrich events. Thus, the synchronous change in water mass distribution and hydrographic cyclicity suggests a possible linkage

  13. Automatic Picking of Foraminifera: Design of the Foraminifera Image Recognition and Sorting Tool (FIRST) Prototype and Results of the Image Classification Scheme

    Science.gov (United States)

    de Garidel-Thoron, T.; Marchant, R.; Soto, E.; Gally, Y.; Beaufort, L.; Bolton, C. T.; Bouslama, M.; Licari, L.; Mazur, J. C.; Brutti, J. M.; Norsa, F.

    2017-12-01

    Foraminifera tests are the main proxy carriers for paleoceanographic reconstructions. Both geochemical and taxonomical studies require large numbers of tests to achieve statistical relevance. To date, the extraction of foraminifera from the sediment coarse fraction is still done by hand and thus time-consuming. Moreover, the recognition of morphotypes, ecologically relevant, requires some taxonomical skills not easily taught. The automatic recognition and extraction of foraminifera would largely help paleoceanographers to overcome these issues. Recent advances in automatic image classification using machine learning opens the way to automatic extraction of foraminifera. Here we detail progress on the design of an automatic picking machine as part of the FIRST project. The machine handles 30 pre-sieved samples (100-1000µm), separating them into individual particles (including foraminifera) and imaging each in pseudo-3D. The particles are classified and specimens of interest are sorted either for Individual Foraminifera Analyses (44 per slide) and/or for classical multiple analyses (8 morphological classes per slide, up to 1000 individuals per hole). The classification is based on machine learning using Convolutional Neural Networks (CNNs), similar to the approach used in the coccolithophorid imaging system SYRACO. To prove its feasibility, we built two training image datasets of modern planktonic foraminifera containing approximately 2000 and 5000 images each, corresponding to 15 & 25 morphological classes. Using a CNN with a residual topology (ResNet) we achieve over 95% correct classification for each dataset. We tested the network on 160,000 images from 45 depths of a sediment core from the Pacific ocean, for which we have human counts. The current algorithm is able to reproduce the downcore variability in both Globigerinoides ruber and the fragmentation index (r2 = 0.58 and 0.88 respectively). The FIRST prototype yields some promising results for high

  14. Increased Amazon freshwater discharge during late Heinrich Stadial 1

    Science.gov (United States)

    Crivellari, Stefano; Chiessi, Cristiano Mazur; Kuhnert, Henning; Häggi, Christoph; da Costa Portilho-Ramos, Rodrigo; Zeng, Jing-Ying; Zhang, Yancheng; Schefuß, Enno; Mollenhauer, Gesine; Hefter, Jens; Alexandre, Felipe; Sampaio, Gilvan; Mulitza, Stefan

    2018-02-01

    The temporal succession of changes in Amazonian hydroclimate during Heinrich Stadial 1 (HS1) (ca. 18-14.7 cal ka BP) is currently poorly resolved. Here we present HS1 records based on isotope, inorganic and organic geochemistry from a marine sediment core influenced by the Amazon River discharge. Our records offer a detailed reconstruction of the changes in Amazonian hydroclimate during HS1, integrated over the basin. We reconstructed surface water hydrography using stable oxygen isotopes (δ18O) and Mg/Ca-derived paleotemperatures from the planktonic foraminifera Globigerinoides ruber, as well as salinity changes based on stable hydrogen isotope (δD) of palmitic acid. We also analyzed branched and isoprenoid tetraether concentrations, and compared them to existing bulk sediment ln(Fe/Ca) data and vegetation reconstruction based on stable carbon isotopes from n-alkanes, in order to understand the relationship between continental precipitation, vegetation and sediment production. Our results indicate a two-phased HS1 (HS1a and HS1b). During HS1a (18-16.9 cal ka BP), a first sudden increase of sea surface temperatures (SST) in the western equatorial Atlantic correlated with the slowdown of the Atlantic Meridional Overturning Circulation (AMOC) and the associated southern hemisphere warming phase of the bipolar seesaw. This phase was also characterized by an increased delivery of terrestrial material. During HS1b (16.9-14.8 cal ka BP), a decrease in terrestrial input was, however, associated with a marked decline of seawater δ18O and palmitic acid δD. Both isotopic proxies independently indicate a drop in sea surface salinity (SSS). A number of records under the influence of the North Brazil Current, in contrast, indicate increases in SST and SSS resulting from a weakened AMOC during HS1. Our records thus suggest that the expected increase in SSS due to the AMOC slowdown was overridden by a two-phased positive precipitation anomaly in Amazonian hydroclimate.

  15. Mõnda Eesti kirjanduselust Moskva vaatevinklist (1958-1986) : [mälestusi ja päevikumärkmeid aastaist 1968-1969] / Vera Ruber

    Index Scriptorium Estoniae

    Ruber, Vera, 1925-2011

    1997-01-01

    Tööst eesti kirjanduse konsultandina Üleliidulise Kirjanike Liidu juhatuse sekretariaadis. Ka 1959. a. romaanivõistlusel silmapaistnud romaanidest (Lilli Prometi "Meesteta küla" ja Raimond Kaugveri "Võõra mõõga teenistuses"), Lilli Prometi romaanist "Primavera" ja venekeelsest kogumikust "Eesti noor proosa" ("Эстонская молодая проза", koost. Endel Mallene ja Rein Saluri, 1978)

  16. Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber

    Czech Academy of Sciences Publication Activity Database

    Siřišťová, L.; Luhový, R.; Sigler, Karel; Řezanka, Tomáš

    2011-01-01

    Roč. 15, č. 3 (2011), 423-429 ISSN 1431-0651 Institutional research plan: CEZ:AV0Z50200510 Keywords : Thermophilic bacteria * Geobacillus * Meiothermus Subject RIV: EE - Microbiology, Virology Impact factor: 2.941, year: 2011

  17. Testing the Molecular Clock Using the Best Fossil Record: Case Studies from the Planktic Foraminifera

    Science.gov (United States)

    Steel, B. A.; Kucera, M.; Darling, K.

    2004-12-01

    Criticism of molecular clock studies often centres on inadequate calibration and a perceived lack of correlation between reproductive isolation and recognisable morphological evolution. Since many major groups (e.g. birds, mammals, reptiles) have a poor fossil record, it is often difficult to test and refute these limitations. Planktic foraminifera represent an exception to this rule. Deep-sea sediments are super-abundant in foraminifera, and large numbers of specimens and occurrences are easily garnered from Ocean Drilling Programme cores. Planktic foraminifera therefore represent an ideal model group with which to test and refine molecular clock studies. Since the 1990AƒAøAøâ_sA¬Aøâ_zAøs, genetic sequences (principally 18S r-RNA) have been extracted from living planktic foraminifera, and a large genetic library has developed. Our study attempts to contextualise and test molecular data, particularly AƒAøAøâ_sA¬Agenetic studies have suggested that these two AƒAøAøâ_sA¬A<Å"super-speciesAƒAøAøâ_sA¬Aøâ_zAø in fact consist of a number of isolated forms, with contrasting ecologies and longevities, which in Recent and sub-Recent sediments can be distinguished either on the basis of pore ultrastructure (Gl. siphonifera) or test colouration (Gs. ruber). In both cases, molecular clock estimates are indicative of ancient (7-11 Ma) intra-species cryptic divergences, which seem to be considerably older than fossil dates. In particular, the calculated molecular split between the two forms of Gs. ruber (AƒAøAøâ_sA¬A<Å"whiteAƒAøAøâ_sA¬Aøâ_zAø and AƒAøAøâ_sA¬A<Å"pinkAƒAøAøâ_sA¬Aøâ_zAø) of around 11 Ma is considerably discordant with the fossil date of around 0.7 Ma. At first glance, this may appear to be a classic case of molecular over-estimation, often a feature of clock models, especially where, as in the foraminifera, substitution rates may vary widely. However, there is good reason to suspect that fossil range of the

  18. Descrição da larva de Scinax similis (Cochran com notas comparativas sobre o grupo "ruber" no sudeste do Brasil (Amphibia, Anura, Hylidae Description of the larva of Scinax similis (Cochran with comparative notes on the Scinax ruber group in Southeastern Brazil (Amphibia, Anura, Hylidae

    Directory of Open Access Journals (Sweden)

    Ana C.R Alves

    1999-06-01

    Full Text Available The larva of Scinax similis (Cochran, 1952 is described from Ilha do Fundão (Rio de Janeiro, Rio de Janeiro State, Brazil. Comparisons with other species of the group that occur in Southeastern Brazil are added. The larva of S. similis can be distinguished by the following diagnostic features: (1 eyes large in relation to body length (17,3%, body height (26,8%, and body width (29,7%, interocular distance three times larger than eye diameter; (2 lower beak with two transverse stripes, proximal half white and distal half black.

  19. Spring feeding by pink-footed geese reduces carbon stocks and sink strength in tundra ecosystems

    NARCIS (Netherlands)

    van der Wal, Rene; Sjogersten, Sofie; Woodin, Sarah J.; Cooper, Elisabeth J.; Jonsdottir, Ingibjorg S.; Kuijper, Dries; Fox, Tony A. D.; Huiskes, A. D.

    Tundra ecosystems are widely recognized as precious areas and globally important carbon (C) sinks, yet our understanding of potential threats to these habitats and their large soil C store is limited. Land-use changes and conservation measures in temperate regions have led to a dramatic expansion of

  20. Spring feeding by pink-footed geese reduces carbon stocks and sink strength in tundra ecosystems

    NARCIS (Netherlands)

    Van der Wal, R.; Sjögersten, S.; Woodin, S.J.; Cooper, E.J.; Jónsdóttir, I.S.; Kuijper, D.; Fox, A.D.; Huiskes, A.H.L.

    2007-01-01

    Tundra ecosystems are widely recognized as precious areas and globally important carbon (C) sinks, yet our understanding of potential threats to these habitats and their large soil C store is limited. Land-use changes and conservation measures in temperate regions have led to a dramatic expansion of

  1. 75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

    Science.gov (United States)

    2010-08-25

    ... identified. No associated funerary objects are present. Based on the skeletal and dental morphology, as well...-mentioned human remains are Native American. Based on the ceramic styles and construction of pottery related...

  2. Is it about "pink" or about "girls"? The inherence heuristic across social and nonsocial domains.

    Science.gov (United States)

    Kinzler, Katherine D; Sullivan, Kathleen R

    2014-10-01

    The inherence heuristic provides an intriguing and novel explanation for early thought in a variety of domains. Exploring similarities and differences in inherent reasoning across social and nonsocial domains can help us understand the role that inherent thinking plays in the development of human reasoning and the process by which more elaborate essentialist reasoning develops.

  3. 75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

    Science.gov (United States)

    2010-08-25

    ... here given in accordance with the Native American Graves Protection and Repatriation Act (NAGPRA), 25 U... Bradley site and have repatriated Native American human remains and associated funerary objects from the... an important burial ritual. Archeological, historical and ethnographic sources indicate that the type...

  4. Marked bengal pink. A dynamic test using marking doses; its value in jaundice diagnosis

    International Nuclear Information System (INIS)

    Kellershohn, C.; Desgrez, A.; Delaloye, B.

    1961-01-01

    The authors do a historic calling back of the differential study of jaundices thanks to the semi logarithmic gradients of expurgation of bromine sulphone-phthalein and thanks to the time of appearance of this material in the bile collected by duodenal tubing. They sum up likewise the foreign works which lead to take the place of this test, the outside count at the level of the head, the liver, and the abdomen after intravenous injection of a running dose of marked Rose Bengal. They specify the technical conditions of this test and report an experience which corroborate its value, its accuracy and the interest of its using in the different types of jaundices (hepatitis and obstructive types). (author) [fr

  5. Pink programming across Europe: exploring identity politics at European LGBT film festivals

    OpenAIRE

    Dhaenens, Frederik

    2018-01-01

    LGBT film festivals curate programs that are expected to cater to LGBT identity politics, conform to normative cinematic standards of European film festivals and consider the manifold desires of their target audiences. Since programmers are crucial to this process, the present study investigated European programmers’ approaches to identity politics. The analysis of in-depth expert interviews with 24 film programmers from 17 film festivals in 17 European countries revealed that most programmer...

  6. Turning the pink cloud grey: Dampening of positive affect predicts postpartum depressive symptoms.

    NARCIS (Netherlands)

    Raes, Filip; Smets, Jorien; Wessel, Ineke; Van Den Eede, Filip; Nelis, Sabine; Franck, Erik; Jacquemyn, Yves; Hanssens, Myriam

    OBJECTIVE: Maladaptive response styles to negative affect have been shown to be associated with prospective (postpartum) depression. Whether maladaptive styles to positive affect are also critically involved is understudied, even though anhedonia (a correlate of low positive affectivity) is a

  7. Characterization of nutrient disorders of gerbera hybrid 'Festival Light Eye Pink'

    Science.gov (United States)

    Gerbera hybrid ‘Festival Yellow with Light Eye’ plants were grown in silica sand culture to induce and photograph nutritional disorder symptoms. Plants were grown with a complete modified Hoagland's all nitrate solution: (macronutrients in mM) 15 NO3-N, 1.0 PO4-P, 6.0 K, 5.0 Ca, 2.0 Mg, and 2.0 SO4...

  8. ‘Hapa White’, 'Hapa Pink', and 'Hapa Red' interspecific hybrid hibiscus cultivars

    Science.gov (United States)

    Hibiscus mutabilis, also known as confederate rose, is native to southeastern China, but it is also grown as an ornamental throughout the southeastern United States and is hardy in USDA zone 7 to 9. It is popular for its large, soft, gray-green foliage during the summer, and large, showy flowers pro...

  9. From the West Wing to Pink Floyd to Einstein Advertising: Astronomy in Popular Culture

    Science.gov (United States)

    Fraknoi, Andrew

    2007-12-01

    In what popular movie does Darryl Hannah play an astronomer? What Japanese car company is named after a well-known star cluster? Can you name at least two murder mysteries that take place at an observatory? What national astronomy education project was mentioned on The West Wing television show (which had several "stealth astronomy” episodes)? What piece of classical music begins with a Big Bang and has the players expanding on stage and into the concert hall? Can you recite the most famous neutrino poem and name the poet? What science fiction story, written by an astronomer under a pseudonym, features an H-R diagram? What rock group had its members’ names included in a reference in the Astrophysical Journal, unbeknownst to the editor? How many astronomy related operas can you name? How many astronomers does it take to screw in a light bulb? Join in on an exploration of astronomy in popular culture, from stamp collecting to advertising, from science fiction (with accurate astronomy) to rock music, from Broadway musicals to modern poetry. Learn which astronomy colleagues have been writing fiction and poetry while you were busy publishing in the research literature. Bring your favorite example of astronomy in popular culture and we'll take the time at the end to share ideas and have some fun. A resource guide for exploring astronomy and popular culture will be available.

  10. Convergence In Latin America: Illuminating The Pink Tide And Iranian Nexus Through Social Network Analysis

    Science.gov (United States)

    2017-12-01

    Global Security Studies 6, no. 3 (Summer 2015): 21. 25 involvement in money laundering , and was a key component of an Iranian financial mechanism...conduct large monetary transactions and money laundering to support the development of what is now called the Rabbani network.115 Rabbani’s final...actual functioning factories.117 The program is assessed to be a money laundering mechanism to move capital from the then- sanctions strapped Iran.118

  11. Pink Time: Evidence of Self-Regulated Learning and Academic Motivation among Undergraduate Students

    Science.gov (United States)

    Baird, Timothy D.; Kniola, David J.; Lewis, Ashley L.; Fowler, Shelli B.

    2015-01-01

    This article describes and analyzes a classroom assignment to promote intrinsic motivation for learning in college students. Here, grades and instructor expectations for content are viewed as students' primary motivations for learning, and correspondingly present obstacles for improved critical thinking skills, student autonomy, and engagement.…

  12. Pink-line syndrome, a physiological crisis in the scleractinian coral Porites lutea

    Digital Repository Service at National Institute of Oceanography (India)

    Ravindran, J.; Raghukumar, C.

    the night and oxidative stress during the day due to the evolution of reactive nascent oxygen by the cyanobacterial photosynthesis. Therefore, in order to stop the photosynthetic processes in the cyanobacterial cells, the photosynthetic inhibitor, 3... and increase in numbers. 15 The subsequent increase in photosynthesis by the increased number of symbionts possibly causes oxidative stress to the host cell. The host is induced to produce defense enzyme such as superoxide dismutase, catalase...

  13. Insidious Influence of Gender Socialization on Females' Physical Activity: Rethink Pink

    Science.gov (United States)

    Mullins, Nicole M.

    2015-01-01

    Continually accumulating information on the health risks associated with sedentary lifestyles indicates a severe public health need for increased physical activity, as well as for careful attention to factors that can curtail it. Study and documentation of such factors, however, are not enough to promote widespread change in firmly established…

  14. Stamina paneb liikuma / Urmo Raiend ; küsis Ülli Pink

    Index Scriptorium Estoniae

    Raiend, Urmo

    2009-01-01

    Spordiklubi Stamina korraldatavatest rahvaspordi üritustest ja -sarjadest: Mayeri Järvejooksud, Eesti Energia kepikõnnisari, Swedbanki Tervisejooksusari, Deloitte'i rahvatriatlonisari, rulluisusari, SL Õhtulehe Rahvajooks

  15. Through Pink and Blue glasses : designing a dispositional empathy game using gender stereotypes and Virtual Reality

    NARCIS (Netherlands)

    Muller, Daphne A.; van Kessel, Caro R.; Janssen, Sam

    2017-01-01

    Society is affected by gender stereotypes and a decrease of dispositional empathy (empathy as a stable character trait). We believe there is an overall lack of awareness how gender stereotypes affect us as individuals, both male and female, and our society as a whole. This paper reports on the

  16. 78 FR 26308 - Endangered and Threatened Wildlife and Plants; Proposed Threatened Status for Coral Pink Sand...

    Science.gov (United States)

    2013-05-06

    ... effects of climate change and drought; and (4) cumulative interaction of individual factors such as off..., we considered the types of activities that might trigger regulatory impacts under the rule, as well... work programs; Child Nutrition; Food Stamps; Social Services Block Grants; Vocational Rehabilitation...

  17. Is a Pink Cow Still a Cow? Individual Differences in Toddlers' Vocabulary Knowledge and Lexical Representations

    Science.gov (United States)

    Perry, Lynn K.; Saffran, Jenny R.

    2017-01-01

    When a toddler knows a word, what does she actually know? Many categories have multiple relevant properties; for example, shape "and" color are relevant to membership in the category "banana." How do toddlers prioritize these properties when recognizing familiar words, and are there systematic differences among children? In…

  18. "Pink Is a Girl's Color": A Case Study of Bilingual Kindergarteners' Discussions about Gender Roles

    Science.gov (United States)

    Kim, So Jung

    2016-01-01

    This article discusses the results of an empirical study that examined young bilingual students' discussions of picture books dealing with gender themes in a Spanish/English bilingual classroom. The study focused on the reading of five picture books by sixteen 5-year-old Mexican-origin children at a small charter school. The data were collected by…

  19. Do Women Prefer Pink? : The Effect of a Gender Stereotypical Stock Portfolio on Investing Decisions

    NARCIS (Netherlands)

    Prast, H.M.; Rossi, M.; Torricelli, C.; Druta, C.

    We investigate whether lack of familiarity may contribute to an explanation of the gender gap in stock market participation and risk taking. We use ads in widely read women magazines to select companies that we assume to be more familiar to women than to men, and construct a “pink” portfolio. We

  20. Chronic effects of nitrogenous compounds on survival and growth of juvenile pink shrimp

    Directory of Open Access Journals (Sweden)

    W. J. Wasielesky

    Full Text Available Abstract In response to growing worldwide market demand, intensive shrimp farming, based on high feed, has developed over the past decade. The nitrogenous compounds mainly generated by animal excretion can cause deterioration of water quality and produce chronic or even acute toxicity to aquatic animals. As prevention, theoretical safety levels have been estimated from acute toxicity tests and they are traditionally used to prevent toxic effects on biota. However, are those concentrations of nitrogenous compounds really safe to Farfantepenaeus paulensis? The current study aimed to investigate the lethal and sublethal effects of ammonia, nitrite and nitrate to juvenile F. paulensis based on safety levels. Each experiment was performed independently in 100 L tanks for 30 days. The survival rates and wet weight of all shrimps were recorded every 10 days. The concentrations tested for ammonia, nitrite and nitrate were respectively: treatment “T1/4”, a quarter of the safety level (0.91 mg/L TA-N, 2.55 mg/L NO2--N and 80.7 mg/L NO3--N; treatment “TSL”, the safety level (3.65 mg/L TA-N, 10.2 mg/L NO2--N and 323 mg/L NO3--N; and treatment “T2X”, twice the safety level (7.30 mg/L TA-N, 20.4 mg/L NO2--N and 646 mg/L NO3--N. For F. paulensis cultivation, the real safety level for nitrite was estimated to be 2.55 mg/L NO2--N. For ammonia and nitrate, the recommended concentrations were <0.91 mg/L TA-N corresponding to 0.045 mg/L NH3-N and <80.7 mg/L NO3--N, respectively.

  1. Do women prefer pink? : The effect of a gender stereotypical stock portfolio on investing decisions

    NARCIS (Netherlands)

    Prast, Henriette; Rossi, M.; Torricelli, C.; Sansone, D.

    2015-01-01

    We investigate whether lack of familiarity may contribute to an explanation of the gender gap in stock market participation and risk taking. We use ads in widely read women magazines to select companies that we assume to be more familiar to women than to men, and construct a “pink” portfolio. We

  2. Contesting the notion of "The Pink Tide", Case studies of Bolivia and Brazil

    DEFF Research Database (Denmark)

    Andersson, Vibeke; Christensen, Steen Fryba

    Vi sammenligner venstredrejningen i Bolivia og Brasilien og diskuterer om disse landes udviklinger er en del af en venstredrejning i Latinamerika.......Vi sammenligner venstredrejningen i Bolivia og Brasilien og diskuterer om disse landes udviklinger er en del af en venstredrejning i Latinamerika....

  3. Chronic effects of nitrogenous compounds on survival and growth of juvenile pink shrimp.

    Science.gov (United States)

    Wasielesky, W J; Poersch, L H; Martins, T G; Miranda-Filho, K C

    2017-01-01

    In response to growing worldwide market demand, intensive shrimp farming, based on high feed, has developed over the past decade. The nitrogenous compounds mainly generated by animal excretion can cause deterioration of water quality and produce chronic or even acute toxicity to aquatic animals. As prevention, theoretical safety levels have been estimated from acute toxicity tests and they are traditionally used to prevent toxic effects on biota. However, are those concentrations of nitrogenous compounds really safe to Farfantepenaeus paulensis? The current study aimed to investigate the lethal and sublethal effects of ammonia, nitrite and nitrate to juvenile F. paulensis based on safety levels. Each experiment was performed independently in 100 L tanks for 30 days. The survival rates and wet weight of all shrimps were recorded every 10 days. The concentrations tested for ammonia, nitrite and nitrate were respectively: treatment "T1/4", a quarter of the safety level (0.91 mg/L TA-N, 2.55 mg/L NO2--N and 80.7 mg/L NO3--N); treatment "TSL", the safety level (3.65 mg/L TA-N, 10.2 mg/L NO2--N and 323 mg/L NO3--N); and treatment "T2X", twice the safety level (7.30 mg/L TA-N, 20.4 mg/L NO2--N and 646 mg/L NO3--N). For F. paulensis cultivation, the real safety level for nitrite was estimated to be 2.55 mg/L NO2--N. For ammonia and nitrate, the recommended concentrations were <0.91 mg/L TA-N corresponding to 0.045 mg/L NH3-N and <80.7 mg/L NO3--N, respectively.

  4. Behavior of Pectinophora gossypiella (gelechiidae) (pink bollworm) males monitored with pheromone trap in cotton

    International Nuclear Information System (INIS)

    Pontes de Melo, Elmo; Degrande, Paulo Eduardo; Aveiro Cessa, Raphael Maia; De Lima Junior, Izidro dos Santos; Barros, Ricardo; Fernandes Nogueira, Rodrigo

    2012-01-01

    The objective of the present study was to evaluate the behavior of p. gossypiella males captured with pheromone-baited traps in cotton field. Three experiments were done during the 2001-02 and 2002-03 growing seasons using the delta opal cotton cultivar. The first experiment was related to the insect population captured during the crop cycle by of two commercially available delta type traps, the second experiment was conducted to evaluate the efficiency of traps in capturing p. gossypiella males and, the third experiment assessed the nocturnal circadian rhythm. It was realized a descriptive analysis of the data collected to first and third experiment. Used in this second experiment consisted of comparing randomly selected groups and the means were compared by t-test, the significance level was set at 5 %, and, the canonical correlation analysis was performed. The delta pheromone trap was more efficient in capturing p. gossypiella than was the PET pheromone trap. Nocturnal activity peaks were found to be related with the time of year and it occurs between 11:00 p.m. and 2:00 a.m.

  5. Pink Collar Blues: For Women Who Work, It Still Doesn't Add Up.

    Science.gov (United States)

    Strum, Philippa

    1980-01-01

    Documents the salary and promotional inequities between women and men in working class, academic, governmental, and professional (law, medicine, and scientific research) jobs. Calls for more affirmative action programs and argues that professional women will be able to fight for equal rights for all classes of working women. (GC)

  6. Pink berry grape (Vitis vinifera L.) characterization: Reflectance spectroscopy, HPLC and molecular markers.

    Science.gov (United States)

    Rustioni, Laura; De Lorenzis, Gabriella; Hârţa, Monica; Failla, Osvaldo

    2016-01-01

    Color has a fundamental role for the qualitative evaluation and cultivar characterization of fruits. In grape, a normally functional pigment biosynthesis leads to the accumulation of a high quantity of anthocyanins. In this work, 28 Vitis vinifera L. cultivars accumulating low anthocyanins in berries were studied to characterize the biosynthetic dysfunctions in both a phenotypic and genotypic point of view. Reflectance spectroscopy, HPLC profiles and molecular markers related to VvMybA1 and VvMybA2 genes allowed a detailed description of the pigment-related characteristics of these cultivars. Data were consistent concerning the heterozygosity of the non-functional allele in both investigated genes, resulting in a low colored phenotype as described by reflectance. However, the variability in berry colour among our samples was not fully explained by MybA locus, probably due to specific interferences among the biosynthetic pathways, as suggested by the anthocyanin profile variations detected among our samples. The results presented in this work confirmed the importance of the genetic background: grapes accumulating high levels of cyanidin-3-O-glucosides (di-substituted anthocyanin) are generally originated by white cultivar retro-mutations and they seem to preserve the anomalies in the flavonoid hydroxylases enzymes which negatively affect the synthesis of tri-substituted anthocyanins. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  7. Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants

    Directory of Open Access Journals (Sweden)

    Cláudia Ulisses

    2010-02-01

    Full Text Available The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM and 2,4-D (0; 13.57; 18.10; 22.62μM or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM. Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be observed, with proembryogenic cell clusters developing into globular embryos. These results offer the possibility of using new types of explants for culturing helicons that avoid the growth of endophytic bacteria.Este trabalho teve como objetivo avaliar a resposta de secções transversais de ovários e o desenvolvimento de calos embriogênicos. O experimento constou de dois ensaios. No primeiro avaliou-se combinações entre AIA (0; 5.71; 8.56; 11.42; 14.27μM e 2,4-D (0; 13.57; 18.10; 22.62μM e no segundo avaliou-se as concentrações de 2,4-D supracitadas, combinadas com concentrações de BA (0; 4.43; 6.65; 8.87; 11.09μM. Os calos formados foram avaliados quanto à presença de estruturas embriogênicas utilizando-se estereomicroscópio, microscópio eletrônico de varredura, além de análises citoquímicas e histológicas. Combinações entre 2,4-D e AIA induziram a formação de calos friáveis com setores pró-embriogênicos, refletindo a aquisição de competência morfogenética. Posteriormente foi observada a expressão do programa embriogênico quando massas pró-embriogências desenvolveram-se formando embriões somáticos. Esses resultados apresentam uma alternativa para a utilização de um tipo de explante que possibilita o cultivo in vitro de helicônia sem o desenvolvimento de bactérias endofíticas.

  8. Through Pink and Blue glasses : Designing a dispositional empathy game using gender stereotypes and Virtual Reality

    NARCIS (Netherlands)

    Muller, Daphne A.; Van Kessel, Caro R.; Janssen, Sam

    2017-01-01

    Society is affected by gender stereotypes and a decrease of dispositional empathy (empathy as a stable character trait). We believe there is an overall lack of awareness how gender stereotypes affect us as individuals, both male and female, and our society as a whole. This paper reports on the

  9. Maturity Stages of Pink Shrimp Farfantepenaeus notialis (Penaeidae in the Colombian Caribbean

    Directory of Open Access Journals (Sweden)

    Daniel Giovanny Pérez Ferro

    2014-05-01

    shrimp commercial vessels in the Colombian Caribbean. The females of F. notialiswere identified and preserved for the histologic analysis and some gonads were fixed. Data of size, weight and sex were taken. The gonadal stages were determined from the morphology and coloration of gonad and to microscopic determination were carried out histologic cut from samples of thirty gonads from all stages. A total of 3019 females were obtained from F. notialis, to which five stages of gonadal development    ESTADIOS DE MADUREZ DEL CAMARÓN ROSADO Farfantepenaeus notialis (PENAEIDAE EN EL CARIBE COLOMBIANO

  10. Assessment of the Phototoxicity of Weathered Alaska North Slope Crude Oil to Juvenile Pink Salmon

    Science.gov (United States)

    Petroleum products are known to have greater toxicity to the translucent embryos and larvae of aquatic organisms in the presence of ultraviolet radiation (UV) compared to toxicity determined in tests performed under standard laboratory lighting with minimal UV. This study assesse...

  11. 77 FR 60207 - Endangered and Threatened Wildlife and Plants; Proposed Threatened Status for Coral Pink Sand...

    Science.gov (United States)

    2012-10-02

    ... FURTHER INFORMATION CONTACT: Larry Crist, Field Supervisor, U.S. Fish and Wildlife Service, Utah Field... burrows, dune migration characteristics that permit vegetation to develop and persist within dune swales... sediment characteristics not conducive for vegetation nor suitable for larval burrows, dune migration...

  12. Assessment of smile architecture and pink aesthetics: A successful methodology in cosmetic dentistry

    OpenAIRE

    Shital Hungund; Dhwani Gohil; Rakesh Mishra

    2012-01-01

    Objective: Aesthetic restorative treatment plays a very essential role in smile appearance. The aim of this study is to assess smile architecture and periodontium outlook for aesthetic evaluation. Materials and Methods: One hundred subjects (50 women and 50 men) aged from 18 to 62 years were enrolled and photographed. Standardized digital photographs were taken to assess smile architecture and gingival biotype during regular and expanded smile. Smile was assessed on the basis of following cri...

  13. Antifungal Compound Isolated from Catharanthus roseus L. (Pink for Biological Control of Root Rot Rubber Diseases

    Directory of Open Access Journals (Sweden)

    R. Zahari

    2018-01-01

    Full Text Available Rigidoporus microporus, Ganoderma philippii, and Phellinus noxius are root rot rubber diseases and these fungi should be kept under control with environmentally safe compounds from the plant sources. Thus, an antifungal compound isolated from Catharanthus roseus was screened for its effectiveness in controlling the growth of these fungi. The antifungal compound isolated from C. roseus extract was determined through thin layer chromatography (TLC and nuclear magnetic resonance (NMR analysis. Each C. roseus of the DCM extracts was marked as CRD1, CRD2, CRD3, CRD4, CRD5, CRD6, and CRD7, respectively. TLC results showed that all of the C. roseus extracts peaked with red colour at Rf = 0.61 at 366 nm wavelength, except for CRD7. The CRD4 extract was found to be the most effective against R. microporus and G. philippii with inhibition zones of 3.5 and 1.9 mm, respectively, compared to that of other extracts. These extracts, however, were not effective against P. noxius. The CRD4 extract contained ursolic acid that was detected by NMR analysis and the compound could be developed as a biocontrol agent for controlling R. microporus and G. philippii. Moreover, little or no research has been done to study the effectiveness of C. roseus in controlling these fungi.

  14. A study in pink : What determines the success of gender-specific advertising?

    NARCIS (Netherlands)

    Fenko, Anna; Drost, Willemijn

    2014-01-01

    Gender-specific marketing gains importance as women become more influential as consumers. The study investigated an efficiency of two approaches to gender-specific marketing: designing a stereotypically “feminine” product and priming an interdependent self-construal with an advertising scenario.

  15. Effect of Cycocel and Salisilic acid on Morphologic Traits of Brassica Oleracea, Pink Type

    Directory of Open Access Journals (Sweden)

    S.N. Mortazavi

    2017-08-01

    Full Text Available Introduction: Brassica Oleracea L. is biennial of cabbages family. This plant is valuable for its leaves and resistivity against cold weather. Cabbages are resistant to cold and suitable for landscape in cold areas. In some ornamental plants, the control of plant size, vegetative growth and size reduction is necessary to enhance sale. Salicylic acid is a phenolic compound that is produced naturally in plants and on multiple factors affect. Cycocel is one of the retarder material to the growth. This research was performed in order to best retarder concentration of growth factors to improve the quantity and quality of ornamental cabbage landscape. Materials and Methods: This study was done as factorial experiment in a randomized complete block design with three replications. Experiment was performed with two factors of cycocel with four levels (0, 50, 100, 150 mg.lit-1 and salicylic acid with four levels ( 0, 50, 100, 200 mg.lit-1 by spraying and every of them with three replications. In this experiment, traits such as plant height, antocianin, resistivity against cold weather, electrolyte leakage, rational component of water, chlorophyll, wet and dry weight of leaves, wet and dry weight of roots and the number of leaves were investigated. 80 numbers of uniform seedlings of ornamental cauliflower varieties of purple were prepared and after the reached the stage of 5 and 4 leaves to the pot of 10 to 15 cm and then after a short growing period for the the main land (bed in loam soil with distance of 30 cm they were transferred. The treatment of salicylic acid and CCC two times and as foliar application on the leaves of cabbage was performed them with an interval 24 to 48 hours. The first stage of treatment on 18 and 19 November and after a few days of planting cabbage and establish them in the land, was performed. Statistical analysis using software MSTATC and comparing the average results using Duncan's multiple range test was performed. Results and Discussion: Result from variance analysis showed that using of cycocel and salisilic acid have effect on growing, antocianin, number of leaves, rational component of water, chlorophyll, net weight of wet and dry leaves and roots, is significant in the level of one percent. The highest percentage of electrolyte leakage, leaf number, leaf and root dry weight and fresh weight were observed at 200 mg/ liter. The plant height, anthocyanin, cold resistance and Fresh weight of root were in control. High levels of chlorophyll and relative water content were observed in 100 mg/ liter. The use of Cycocel had a significant effect on growth traits. The highest dry weight of leaves, root dry weight and the highest percentage of electrolyte leakage in the treatment of 150 mg/ liter and the highest plant height, the highest percentage of relative water content and chlorophyll was observed in control. Most of the leaves, anthocyanin and cold resistance at the level of 50 mg/litter and highest fresh weight were observed at 100 mg/litter. The cycocel had no significant effect the growth traits. Results showed that significant interactions SA and CCC one percent of levels on plant height, electrolyte leakage, number of leaves, anthocyanins, relative water content, chlorophyll content, leaf dry weight and root dry weight and the significant other traits has not. The results showed that spray application separately SA and CCC and the interaction of these compounds had significant effects on the plant height, anthocyanin extract, chlorophyll conten, fresh weight of root and dry weight of root. The results showed that the Cycocel and interaction of SA and CCC had a significant effect on the relative water content, but salicylic acid alone had no significant effect, which may be no significant in this experiment due to lack of relative water content in the presence of a low level of salicylic acid and or the duration of treatment was low. Cycocel alone and combination of SA and CCC had significant effects on dry weight of leaves, but salicylic acid alone had no significant effect. Conclusions: Therefore, to increase the quantity and quality characteristics of ornamental cabbage treatments Salyslyk acid concentration of 200 mg l cycocel concentration of 150 mg per liter is recommended

  16. Choosing Wisely When It Comes to Eye Care: Antibiotics for Pink Eye

    Science.gov (United States)

    ... Laundry Packets On the Rise Jun 30, 2017 New Technology Helps the Legally Blind Be More Independent Oct 31, 2016 Is El Niño Making Your Allergies Worse? May 16, 2016 More Eye Health News Stem Cells May Return Some Vision Lost to Wet AMD ...

  17. The effect of solvents on recovery of polyphenols from the pink fuji ...

    African Journals Online (AJOL)

    Flavonoids constitute a group of polyphenols widely distributed in plants and are assumed to have beneficial effects on human health when present in food. The phenolic content of apple fruit skin and leaves was determined at the developmental stage of each organ. Phenolic levels decreased on a dry weight basis during ...

  18. Water Isotope Proxy-Proxy and Proxy-Model Convergence for Late Pleistocene East Asian Monsoon Rainfall Reconstructions

    Science.gov (United States)

    Clemens, S. C.; Holbourn, A.; Kubota, Y.; Lee, K. E.; Liu, Z.; Chen, G.

    2017-12-01

    Confidence in reconstruction of East Asian paleomonsoon rainfall using precipitation isotope proxies is a matter of considerable debate, largely due to the lack of correlation between precipitation amount and isotopic composition in the present climate. We present four new, very highly resolved records spanning the past 300,000 years ( 200 year sample spacing) from IODP Site U1429 in the East China Sea. We demonstrate that all the orbital- and millennial-scale variance in the onshore Yangtze River Valley speleothem δ18O record1 is also embedded in the offshore Site U1429 seawater δ18O record (derived from the planktonic foraminifer Globigerinoides ruber and sea surface temperature reconstructions). Signal replication in these two independent terrestrial and marine archives, both controlled by the same monsoon system, uniquely identifies δ18O of precipitation as the primary driver of the precession-band variance in both records. This proxy-proxy convergence also eliminates a wide array of other drivers that have been called upon as potential contaminants to the precipitation δ18O signal recorded by these proxies. We compare East Asian precipitation isotope proxy records to precipitation amount from a CCSM3 transient climate model simulation of the past 300,000 years using realistic insolation, ice volume, greenhouse gasses, and sea level boundary conditions. This model-proxy comparison suggests that both Yangtze River Valley precipitation isotope proxies (seawater and speleothem δ18O) track changes in summer-monsoon rainfall amount at orbital time scales, as do precipitation isotope records from the Pearl River Valley2 (leaf wax δ2H) and Borneo3 (speleothem δ18O). Notably, these proxy records all have significantly different spectral structure indicating strongly regional rainfall patterns that are also consistent with model results. Transient, isotope-enabled model simulations will be necessary to more thoroughly evaluate these promising results, and to

  19. Late Pleistocene shallow water sand transported to the slope at IODP Sites U1484 and U1485 off the north coast of Papua New Guinea: how, when and why?

    Science.gov (United States)

    Mountain, G. S.; Browning, J. V.; Bova, S. C.

    2017-12-01

    IODP Exp 363 drilled two sites on a gently seaward-dipping terrace 18 and 21 km north of Papua New Guinea, enabling the study of mechanisms that bring shallow water sediment to the deep sea. We expect past changes in sea level and precipitation / fluvial run-off dominated this record, but additional processes may have been important. We examined Hole U1484B (1031 m water depth; 223 m drilled; 99.8% recovered) and detected 339 sharp-based sand layers 0.5 cm or more thick. In contrast to the background hemipelagic nanno-bearing silty clay, sand layers are graded or massive turbidites containing detrital grains, shallow-water benthic foraminifera, shell fragments and/or wood. δ18O values of Globigerinoides ruber tied to the isotopic curve of Lisiecki and Raymo (2004) show the densest concentration of sand layers in the last 310 ka occurred during the cooling trend of MIS stage 6. Stage 2 contains significantly fewer discrete sand beds, even during the coldest part of the LGM. Other times of glacial intensification show a similarly modest correlation to peak sand deposition. Sand layers strongly correlate with high values of magnetic susceptibility (MS) measured on unsplit cores, and when mapped to the MIS time scale, MS increases match times of ice growth / falling sea level more consistently than does the density of sand layers. We attribute this to reworking of discrete sand layers by bioturbation, indicating the need for caution tying the absence of sharp-based sands to times of transgression or low precipitation / fluvial run-off. Packages of especially thick and closely-spaced sharp-based sands match seismic reflections at Site U1484. Tracing these reflections throughout the grid of hi-res MCS site survey profiles reveals the areal distribution and transport path of sand as well as a direct tie to similar sharp-based sands in the more distal Hole U1485A (1145 m water depth; 301 m drilled; 103.8% recovered.) The distribution of sands through time might be

  20. Centennial-scale links between Atlantic Ocean dynamics and hydroclimate over the last 4400 years: Insights from the northern Gulf of Mexico

    Science.gov (United States)

    Thirumalai, K.; Quinn, T. M.; Okumura, Y.; Richey, J. N.; Partin, J. W.; Poore, R. Z.

    2015-12-01

    Surface circulation in the Atlantic Ocean is an important mediator of global climate and yet its variability is poorly constrained on centennial timescales. Changes in the Atlantic meridional overturning circulation (AMOC) have been implicated in late Holocene climate variability in the Western Hemisphere, although the relationship between AMOC variability and hydroclimate is uncertain due to the lack of sufficiently highly resolved proxy records. Here we present a replicated reconstruction of sea-surface temperature (SST) and salinity (SSS) from the Garrison Basin in the northern Gulf of Mexico (NGOM) spanning the last 4,400 years to better constrain past sea-surface conditions. We generated time series of paired Mg/Ca (SST proxy) and δ18O (SST and SSS proxy) variations in planktic foraminifer Globigerinoides ruber (white variety) from three multi-cores collected in 2010. Using a Monte Carlo-based technique we produce a stacked record from the three multi-cores and constrain analytical, calibration, chronological, and sampling uncertainties. We apply this technique to existing paired Mg/Ca- δ18O studies in the Gulf of Mexico and Atlantic Ocean to facilitate comparison between time-uncertain proxy reconstructions. The Garrison Basin stack exhibits large centennial-scale variability (σSST~0.6°C; δ18Osw~0.17‰) and indicates a substantially cool (0.9±0.5°C) and fresh (0.26±0.1‰) Little Ice Age (LIA; 1450-1850 A.D.), corroborating extant records from the Gulf of Mexico. Focusing on the last millennium, we analyze a suite of oceanic and terrestrial proxy records to demonstrate a centennial-scale link between salt advection in the Atlantic Ocean, a diagnostic parameter of ocean circulation, and hydroclimate in the adjacent continents. The ensuing multiproxy relationships seem to be consistent with spatial field correlations of limited salinity and rainfall instrumental/reanalysis data, which suggest that NGOM salinity varies with large-scale Atlantic Ocean

  1. Sediment provenance in the Laxmi Basin of the Arabian Sea during the last 800 kyrs

    Science.gov (United States)

    Khim, B. K.; Horikawa, K.; Asahara, Y.; Kim, J. E.; Ikehara, M.; Lee, J.

    2017-12-01

    International Ocean Discovery Program Expedition 355 conducted to drill 1109.4 m penetration at Site U1456 in the Laxmi Basin of the Arabian Sea. Four lithologic units are defined onboard at Site U1456 (Pandey et al., 2016). Unit I is 121 m long, consisting mostly of pelagic carbonates (nannofossil ooze and/or foraminifera-rich nannofossil ooze) interbedded with thin terrigenous (clay, silt, and sand) turbidite layers. The age model of Unit I was determined by the correlation of δ18O fluctuations of planktonic foraminifera (Globigerinoides ruber) to LR04 stacks, estimating 1.2 Ma. A total of 60 samples, collected in the context of magnetic susceptibility (MS) changes at a discrete interval from a composite section (Holes U1456A and U1456C) of Unit I, were analyzed to measure Nd and Sr isotopes of detrital fraction. Based on Nd and Sr isotopes, the sediment provenance in the Laxmi Basin during the last 800 kyrs was traced in response to the monsoon activity between the interglacial and glacial periods. ɛNd and 87Sr/86Sr vary in a range from -12.4 to -8.0 and from 0.712 to 0.727, respectively. The correlation between ɛNd and 87Sr/86Sr is quite linear, indicating that the sediments were provided mainly by two dominant sources. Considering the ɛNd and 87Sr/86Sr end-members of sediment sources (i.e., river sediments), the Tapi River and Narmada River are the main contributors of sediments to Site U1456 with a little influence by the modern Indus River. However, the glacial sediments from the Indus River and the Mahi River may supply an additional fraction, leading to less ɛNd and more 87Sr/86Sr at Site U1456. Judged by the sediment sources, the sediments in the Laxmi Basin are characterized by the mixture of different provenances. In addition, it should be noted that the low ɛNd and high 87Sr/86Sr values coincide largely with high MS and vice versa, irrespectively of the glacial-interglacial change. Thus, rather than the sediment provenances, ɛNd and 87Sr/86Sr

  2. Investigation of Possible Tsunami Events on the Eastern Coast of Taiwan: Case Studies of Lu-Ye, Changping, and Tulan.

    Science.gov (United States)

    Laksono, A. T., Jr.; Tsai, L. L. Y., Sr.

    2017-12-01

    Major earthquakes had occurred in eastern Taiwan for many times. According to an Amis folklore originated in Chengkong City, there was a big sea wave struck their settlement in 1850 AD. Several studies had been conducted, however, the evidence which indicates the tsunami was very weak. There is also a possibility that big sea waves had occurred due to typhoons which take place 3-4 times a year in Taiwan. The purpose of this study is to prove the possibility of tsunami events on the eastern coast of Taiwan based on sedimentological features. The methods in this study are facies analysis including observation of the marine terrace along Lu-Ye, Changping, and Tulan, identification of lithology, sedimentary structure, and fossil content. Lithology analysis is conducted by using point counting of 12 sandstone samples from marine terrace outcrops. Based on the field observation, we found a thin sand marine deposit included in the beach gravel at a height of 10 meters at the Changping marine terrace. It contains coral and some Mollusca shells and does not display any particular sedimentary structure. Sediments that have similar characteristics were also found in the Tulan marine terrace with a height of 5 m. In addition, fossil analysis of marine sand in Tulan exhibits the presence of several planktonic foraminifera fossils such as Orbulina bilobata and Globigerinoides ruber. Temporary interpretation indicates that there is a "super" event which transports shallow marine and beach materials subsequently deposit them on top of an alluvial fan. A 10 cm thin layer of sediment serves as an early tsunami indicator. In addition, the absence of deposits with the same characteristics further indicates that the event occurred only once. Since the eastern coast of Taiwan is an uplift zone with an uplift rate between 5-8 mm/year, the estimated wave height of tsunami should take into account both the tsunami age and the uplift rate. Furthermore, based on the distance from the

  3. Late Pliocene History of Mediterranean Outflow Water in the Gulf of Cadiz (IODP Expedition 339, Site U1389)

    Science.gov (United States)

    Grunert, P.; Balestra, B.; Flores, J. A.; Sierro, F. J.; García Gallardo, Á.; Piller, W. E.; Aubry, M. P.

    2014-12-01

    The opening and closure of ocean gateways play an important role amongst climate forcing mechanisms: surface and deep-water circulation are altered, and hence global heat transport. An important component of North Atlantic circulation patterns is the warm and saline Mediterranean Outflow Water (MOW) that enters the North Atlantic via the Gibraltar Strait. Its onset and early history after the opening of the Gibraltar Strait at 5.3 Ma are poorly constrained and its impact on oceanography and climate in the Pliocene are largely unexplored. Current research efforts in the scope of projects funded by the Austrian Science Fund (FWF-P25831-N29) and the Max Kade Foundation aim to improve our knowledge about this early phase of MOW. Research focuses on Sites U1387C (upper Miocene-lower Pliocene; ~5.8-3.8 Myrs) and U1389E (middle-upper Pliocene; ~3.7-2.8) drilled during IODP Expedition 339 in the northern Gulf of Cádiz close to the source area of MOW. At IODP Site U1389E, a 280m-thick sequence of upper Pliocene contourites is studied to reconstruct MOW behavior and potential paleoceanographic and paleoclimatic feedback mechanisms during the Mid-Pliocene Thermal Optimum. Currently, a reliable age model is developed for the studied interval based on bio-, chemo-, and cyclostratigraphy. Several biostratigraphic datums have been identified from analyses of calcareous nannoplankton and planktic foraminifera which indicate a depositional age between 3.65 Ma and 2.87 Ma. In some intervals of the record a further refinement of the age model at the millenial scale is complicated by poor recovery. However, preliminary results from high-resolution geochemical analyses of the bulk sediment (TOC, CaCO3 and S) and shells of the planktic foraminifer Globigerinoides ruber (δ18O, δ13C) reveal distinct cyclic patterns in several well recovered intervals. Based on an average sedimentation rate of ~25cm/ka, these cycles most likely reflect precessional forcing. Current paleoceanographic

  4. Variations in Mediterranean-Atlantic exchange across the late Pliocene climate transition

    Science.gov (United States)

    García-Gallardo, Ángela; Grunert, Patrick; Piller, Werner E.

    2018-03-01

    Mediterranean-Atlantic exchange through the Strait of Gibraltar plays a significant role in the global ocean-climate dynamics in two ways. On one side, the injection of the saline and warm Mediterranean Outflow Water (MOW) contributes to North Atlantic deep-water formation. In return, the Atlantic inflow is considered a sink of less saline water for the North Atlantic Ocean. However, while the history of MOW is the focus of numerous studies, the Pliocene Atlantic inflow has received little attention so far. The present study provides an assessment of the Mediterranean-Atlantic exchange with a focus on the Atlantic inflow strength and its response to regional and global climate from 3.33 to 2.60 Ma. This time interval comprises the mid-Pliocene warm period (MPWP; 3.29-2.97 Ma) and the onset of the Northern Hemisphere glaciation (NHG). For this purpose, gradients in surface δ18O records of the planktonic foraminifer Globigerinoides ruber between the Integrated Ocean Drilling Program (IODP) Hole U1389E (Gulf of Cádiz) and Ocean Drilling Program (ODP) Hole 978A (Alboran Sea) have been evaluated. Interglacial stages and warm glacials of the MPWP revealed steep and reversed (relative to the present) W-E δ18O gradients suggesting a weakening of Mediterranean-Atlantic exchange likely caused by high levels of relative humidity in the Mediterranean region. In contrast, periods of stronger inflow are indicated by flat δ18O gradients due to more intense arid conditions during the severe glacial Marine Isotope Stage (MIS) M2 and the initiation of NHG (MIS G22, G14, G6-104). Intensified Mediterranean-Atlantic exchange in cold periods is linked to the occurrence of ice-rafted debris (IRD) at low latitudes and a weakening of the Atlantic Meridional Overturning Circulation (AMOC). Our results thus suggest the development of a negative feedback between AMOC and exchange rates at the Strait of Gibraltar in the latest Pliocene as it has been proposed for the late Quaternary.

  5. Composition of Hydrothermal Vent Microbial Communities as Revealed by Analyses of Signature Lipids, Stable Carbon Isotopes and Aquificales Cultures

    Science.gov (United States)

    Jahnke, Linda L.; Edger, Wolfgang; Huber, Robert; Hinrichs, Kai-Uwe; Hayes, John M.; DesMarais, David J.; Cady, Sherry; Hope, Janet M.; Summons, Roger E.; DeVincenzi, Donald L. (Technical Monitor)

    2001-01-01

    Extremely thermophilic microbial communities associated with the siliceous vent walls and outflow channel of Octopus Spring, Yellowstone National Park, have been examined for lipid biomarkers and carbon isotopic signatures. These data were compared with that obtained from representatives of three Aquificales genera. Thermocrinis ruber. "Thermocrinis sp. HI", Hydrogenobacter thermophilus TK-6, Aquifex pyrophilus and Aquifex aeolicus all contained phospholipids composed not only of the usual ester-linked fatty acids, but also ether-linked alkyls. The fatty acids of all cultured organisms were dominated by a very distinct pattern of n-C-20:1 and cy-C-21 compounds. The alkyl glycerol ethers were present primarily as CIS() monoethers with the expection of the Aquifex spp. in which dialkyl glycerol ethers with a boarder carbon-number distribution were also present. These Aquificales biomarker lipids were the major constituents in the lipid extracts of the Octopus Spring microbial samples. Two natural samples, a microbial biofilm growing in association with deposition of amorphous silica on the vent walls at 92 C, and the well-known 'pink-streamers community' (PSC), siliceous filaments of a microbial consortia growing in the upper outflow channel at 87 C were analyzed. Both the biofilm and PSC samples contained mono and dialkyl glycerol ethers with a prevalence of C-18 and C-20 alkyls. Phospholipid fatty acids were comprised of both the characteristic Aquificales n-C-20:1 and cy-C-21, and in addition, a series of iso-branched fatty acids from i-C-15:0 to i-C-21:0, With i-C-17:0 dominant in the PSC and i-C-19:0 in the biofilm, suggesting the presence of two major bacterial groups. Bacteriohopanepolyols were absent and the minute quantities of archaeol detected showed that Archaea were only minor constituents. Carbon isotopic compositions of the PSC yielded information about community structure and likely physiology. Biomass was C-13-depleted (10.9%) relative to available

  6. Boron isotope-based seasonal paleo-pH reconstruction for the Southeast Atlantic - A multispecies approach using habitat preference of planktonic foraminifera

    Science.gov (United States)

    Raitzsch, Markus; Bijma, Jelle; Benthien, Albert; Richter, Klaus-Uwe; Steinhoefel, Grit; Kučera, Michal

    2018-04-01

    The boron isotopic composition of planktonic foraminiferal shell calcite (δ11BCc) provides valuable information on the pH of ambient water at the time of calcification. Hence, δ11BCc of fossil surface-dwelling planktonic foraminifera can be used to reconstruct ancient aqueous pCO2 if information on a second carbonate system parameter, temperature and salinity is available. However, pH and pCO2 of surface waters may vary seasonally, largely due to changes in temperature, DIC, and alkalinity. As also the shell fluxes of planktonic foraminifera show species-specific seasonal patterns that are linked to intra-annual changes in temperature, it is obvious that δ11BCc of a certain species reflects the pH and thus pCO2 biased towards a specific time period within a year. This is important to consider for the interpretation of fossil δ11BCc records that may mirror seasonal pH signals. Here we present new Multi-Collector Inductively Coupled Mass Spectrometry (MC-ICPMS) δ11BCc coretop data for the planktonic foraminifera species Globigerina bulloides, Globigerinoides ruber, Trilobatus sacculifer and Orbulina universa and compare them with δ11Bborate derived from seasonally resolved carbonate system parameters. We show that the inferred season-adjusted δ11BCc /δ11Bborate relationships are similar to existing calibrations and can be combined with published δ11BCc field and culture data to augment paleo-pH calibrations. To test the applicability of these calibrations, we used a core drilled on the Walvis Ridge in the Southeast Atlantic spanning the last 330,000 years to reconstruct changes in surface-water pCO2. The reconstruction based on G. bulloides, which reflects the austral spring season, was shown to yield values that closely resemble the Vostok ice-core data indicating that surface-water pCO2 was close to equilibrium with the atmosphere during the cooler spring season. In contrast, pCO2 estimated from δ11BCc of O. universa, T. sacculifer and G. ruber that

  7. Dichelyne (Dichelyne) spinigerus sp. nov. (Nematoda: Cucullanidae) from the marine fish Otolithes ruber (Sciaenidae) off Iran and first description of the male of Philometra otolithi Moravec et Manoharan, 2013 (Nematoda: Philometridae)

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Khosheghbal, M.; Pazooki, J.

    2014-01-01

    Roč. 59, č. 2 (2014), s. 229-237 ISSN 1230-2821 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Dichelyne * Philometra * marine fish * Otolithes * Iran Subject RIV: EG - Zoology Impact factor: 0.905, year: 2014

  8. Responsiveness to distracting stimuli, though increased in Parkinson's disease, is decreased in asymptomatic PINK1 and Parkin mutation carriers

    DEFF Research Database (Denmark)

    Verleger, Rolf; Hagenah, Johann; Weiss, Manuel

    2010-01-01

    Patients with Parkinson's disease (PD) are more sensitive than healthy controls to response-triggering by irrelevant flanking stimuli in speeded choice-response tasks. This increased responsiveness may either indicate a lack of executive control or reflect compensatory efforts to cope...... motor system. Disease progression might prevent symptomatic PD patients from using this compensatory mechanism, leading to increased disinhibition of their lateral motor system....

  9. Postharvest shelf-life extension of pink guavas (Psidium guajava L.) using HPMC-based edible surface coatings.

    Science.gov (United States)

    Vishwasrao, Chandrahas; Ananthanarayan, Laxmi

    2016-04-01

    Psidium guajava L. var. 'Lalit' is a perishable fruit with delicate skin which is prone to damage. The objective of this study was to determine the effect of edible coating made up of hydroxypropyl methyl cellulose and palm oil on ripening of guava. Coating solution was applied over fruits and coated fruits were stored at 24 ± 1 °C and 65 ± 5%RH. Changes in fruit colour, texture softening, respiration rate, weight loss, ascorbic acid content, soluble solids, titrable acidity, chlorophyll content, total reducing sugars, total phenolic content were studied during post-harvest ripening. Fruits coated with 1 % of hydroxypropyl methyl cellulose and 0.3 % of palm oil showed significant delay in weight loss, fruit firmness as well as colour change (p < 0.05). Coating delayed the enzyme activities of peroxidase and polyphenol oxidase of the fruit. Results suggest that overall quality of coated fruit was maintained by edible coating formulation extending the shelf life of fruit up to 12 days with appreciable retention of all quality parameters tested.

  10. The pink journalism like not-fiction soap opera within the framework of the freedom of expression

    Directory of Open Access Journals (Sweden)

    Dr. Carlos Pérez Ariza

    2007-01-01

    Full Text Available This article aims to describe the yellow journalism phenomenon in Spain during recent years. We hypothesize that such sensationalism is instaled within the so called ‘gossip press’. We made a brief one, although exhaustive relation of the meaning of the yellow media or vein sensationalist in its American origins, whose objective era and is to sell newspaper, as well as timid and little the excellent attempts of this form of journalism in Spain. Its development, harnessed by the television, comes dice by the proliferation from programs to the use in the channels deprived public and, as well as the increase of the circulation of magazines of the heart, as much the traditional ones as Hello to most recent because You say to me, among others. We assert that the practice of this peculiar way of information very often goes beyond the limits of the citizen’s rights to be truthfully informed, to the expression of freedom, both of information authors as the audiences. In the same way, we face such a journalistic exercise in front of its own structure, that is close to the fiction metastory in which TV serials are based, here adopting the shape of a no-fiction discourse

  11. The Pink Underside: The Commercialization of Medical Risk Assessment and Decision-Making Tools for Hereditary Breast Cancer Risk.

    Science.gov (United States)

    Hesse-Biber, Sharlene; Flynn, Bailey; Farrelly, Keeva

    2018-04-01

    The growth of the Internet since the millennium has opened up a myriad of opportunities for education, particularly in medicine. Although those looking for health care information used to have to turn to a face-to-face doctor's visit, an immense library of medical advice is now available at their fingertips. The BRCA genetic predispositions (mutations of the BRCA1 and BRCA2 breast cancer genes) which expose men and women to greater risk of breast, ovarian, and other cancers can be researched extensively online. Several nonprofit organizations now offer online risk assessment and decision-making tools meant to supplement conversation with medical professionals, which in actuality are quickly replacing it. We argue here through a critical qualitative template analysis of several such tools that the discursive frameworks utilized are prone to fearmongering, commercialization, and questionable validity. Left unchecked, these assessment tools could do more harm than good in driving young women especially to take unnecessary extreme surgical action.

  12. Effects of sowing time on pink snow mould, leaf rust and winter damage in winter rye varieties in Finland

    Directory of Open Access Journals (Sweden)

    M. SERENIUS

    2008-12-01

    Full Text Available Disease infection in relation to sowing time of winter rye (Secale cereale was studied in southern Finland in order to compare overwintering capacity of modern rye varieties and to give recommendations for rye cultivation. This was done by using three sowing times and four rye varieties in field trials conducted at three locations in 1999–2001. The early sown rye (beginning of August was severely affected by diseases caused by Puccinia recondita and Microdochium nivale, whereas postponing sowing for two weeks after the recommended sowing time resulted in considerably less infection. The infection levels of diseases differed among rye varieties. Finnish rye varieties Anna and Bor 7068 were more resistant to snow mould and more winter hardy than the Polish variety Amilo, or the German hybrid varieties Picasso and Esprit. However, Amilo was the most resistant to leaf rust. In the first year snow mould appeared to be the primary cause of winter damage, but in the second year the winter damage was positively correlated with leaf rust. No significant correlation between frit fly infestation and winter damage or disease incidence of snow mould or leaf rust was established. The late sowing of rye (in the beginning of September is recommended in Finland, particularly with hybrid varieties, to minimize the need for chemical plant protection in autumn.;

  13. Influence of Citric Acid on the Pink Color and Characteristics of Sous Vide Processed Chicken Breasts During Chill Storage

    Science.gov (United States)

    Lim, Ki-Won

    2015-01-01

    Chicken breast dipped with citric acid (CA) was treated by sous vide processing and stored in a refrigerated state for 0, 3, 6, 9, and 14 d. A non-dipped control group (CON) and three groups dipped in different concentrations of citric acid concentration were analyzed (0.5%, 0.5CIT; 2.0%, 2CIT and 5.0%, 5CIT; w/v). Cooking yield and moisture content increased due to the citric acid. While the redness of the juice and meat in all groups showed significant increase during storage, the redness of the citric acid groups was reduced compared to the control group (pacid reactive substances (TBARS) were generally lower in the citric acid-treated samples than in untreated ones, indicating extended shelf life of the cooked chicken breast dipped in citric acid solution. The shear force of the 2CIT and 5CIT groups was significantly lower (pcitric acid concentrations. PMID:26761885

  14. A field study of the geomorphic effects of sublimating CO2 blocks on dune slopes at Coral Pink Dunes, Utah.

    Science.gov (United States)

    Bourke, Mary; Nield, Jo; Diniega, Serina; Hansen, Candy; McElwaine, Jim

    2016-04-01

    The seasonal sublimation of CO2 ice is an active driver of present-day surface change on Mars. Diniega et al (2013) proposed that a discrete type of Martian gully, found on southern hemisphere dunes, were formed by the movement of CO2 seasonal ice blocks. These 'Linear Gullies' consist primarily of long (100 m - 2.5 km) grooves with near-uniform width (few-10 m wide), and typical depth of nudge it moved downslope. The dynamics of the block movement were recorded using a pair of high resolution video cameras. Geomorphological observations were noted and topographic change was quantified using a Leica P20 terrestrial laser scanner with a resolution of 0.8 mm at 10 m, and change detection limits less than 3 mm. The block run was repeated a total of 10 times and launched from the same location at the dune brink. The experiment ran for 45 minutes. The block size was reduced to (45 x 190 x 195 mm) by the end of the run series. The resultant geomorphology shows that the separate block runs occupied different tracks leading to a triangular plan form shape with a maximum width of 3.5 m. This is different from the findings in Arizona where a narrower track span was recorded (1.7m) (Bourke et al, 2016). Similar block dynamics were observed at both sites (as blocks moved straight, swiveled and bounced downslope). Distinctive pits with arcuate rims on their downslope edge were formed where blocks bounced on the surface. These pits are at an almost equidistant spacing. Despite a longer slope (16 m as opposed to 8m at Grand Falls), no depositional apron was formed. Levee development was less consistent compared to the Arizona site, but a pronounced unpaired-levee formed towards the base of the lee slope. These data show that sublimating blocks of CO2 ice leave signatures of transport paths and are capable of eroding and transporting sediment. Diniega,S. et al (2013) A new dry hypothesis for the formation of Martian linear gullies. Icarus. Vol. 225, 1, p. 526-537. Bourke, M.C. et al (2016) The geomorphic effect of sublimating CO2 blocks on dune lee slopes at Grand Falls, Arizona. LPSC

  15. Ten-year variations in population structure of pink-shrimp in a southwestern Atlantic Bay affected by highway construction

    Directory of Open Access Journals (Sweden)

    Felipe Freitas Jr

    2011-12-01

    Full Text Available OBJECTIVES: Population structures of Farfantepenaeus brasiliensis and F. paulensis, consisting mainly of juveniles, were evaluated during the construction of an expressway along a mangrove area. Estuarine regions in southern Brazil function as sites for reproduction, nursery and growth of a variety of organisms, including two of the most important species of shrimp for fisheries in the southwestern Atlantic. Shrimps were collected in Saco dos Limões creek, Baía-Sul, Florianópolis, State of Santa Catarina, four times a year, by day and night, between 1997 and 2006. Fluctuations in the abundances of shrimp appear to be related to their life cycles, with largest abundances in Summer and Spring, rather than to impacts promoted by dredging activities during the expressway construction.OBJETIVOS: A estrutura populacional de Farfantepenaeus brasiliensis e F. paulensis, consistindo principalmente de juvenis, foi avaliada durante a construção de uma via expressa ao longo de uma área de manguezais. Regiões estuarinas do sul do Brasil funcionam como locais de reprodução, berçários e de crescimento de uma variedade de organismos, incluindo duas das mais importantes espécies de camarões comerciais do Atlântico Sul Ocidental. Camarões foram coletados no Saco dos Limões, Baía Sul, Florianópolis, Estado de Santa Catarina, trimestralmente, de dia e de noite, entre 1997 e 2006. Flutuações em abundâncias de camarões podem estar relacionados aos seus ciclos de vida, com as maiores abundâncias no verão e primavera, ao invés de impactos promovidos pelas atividades de dragagem durante a construção da via expressa.

  16. Living in isolation - population structure, reproduction, and genetic variation of the endangered plant species Dianthus gratianopolitanus (Cheddar pink).

    Science.gov (United States)

    Putz, Christina M; Schmid, Christoph; Reisch, Christoph

    2015-09-01

    The endangered plant species Dianthus gratianopolitanus exhibits a highly fragmented distribution range comprising many isolated populations. Based upon this pattern of distribution, we selected a study region in Switzerland with a lower magnitude of isolation (Swiss Jura) and another study region in Germany with a higher degree of isolation (Franconian Jura). In each region, we chose ten populations to analyze population structure, reproduction, and genetic variation in a comparative approach. Therefore, we determined population density, cushion size, and cushion density to analyze population structure, investigated reproductive traits, including number of flowers, capsules, and germination rate, and analyzed amplified fragment length polymorphisms to study genetic variation. Population and cushion density were credibly higher in German than in Swiss populations, whereas reproductive traits and genetic variation within populations were similar in both study regions. However, genetic variation among populations and isolation by distance were stronger in Germany than in Switzerland. Generally, cushion size and density as well as flower and capsule production increased with population size and density, whereas genetic variation decreased with population density. In contrast to our assumptions, we observed denser populations and cushions in the region with the higher magnitude of isolation, whereas reproductive traits and genetic variation within populations were comparable in both regions. This corroborates the assumption that stronger isolation must not necessarily result in the loss of fitness and genetic variation. Furthermore, it supports our conclusion that the protection of strongly isolated populations contributes essentially to the conservation of a species' full evolutionary potential.

  17. Photocatalytic Oxidation and Reduction Chemistry and a New Process for Treatment of Pink Water and Related Contaminated Water

    Science.gov (United States)

    1996-10-01

    Influence of pH on the Degradation Kinetics of Nitrophenol Isomers in a Heterogeneous ...34 Heterogeneous Photocatalytic Degradation of Organic Water Contaminants: Kinetics and Hydroxyl Radical Mechanisms," Ph.D. Thesis, Raleigh, NC: North Carolina...Dioxide Systems : Photocatalytic Degradation of Chloro- and Nitrophenols ." Trace Met. Environ 3, no. Photocatalytic Purification and Treatment of Water

  18. The "hour of pink twilight": lesbian poetics and queer encounters on the fin-de-siècle street.

    Science.gov (United States)

    Flint, Kate

    2009-01-01

    This essay examines the cultural representation of women's encounters on the fin-de-siècle street and, in particular, the uncertainties that clustered around the possibilities of mutual, or one-sided, same-sex desire accompanying such meetings. It argues, through an examination of lyric poetry, paintings, and short fiction, for the usefulness of twilight--a time of shadowy ambiguity--as a trope to suggest these uncertainities. More than this, it maintains that the lyric and the developing genre of the short story were modes ideally suited to an invocation of the fluid, the uncertain, and the unnamable. This argument is advanced through a close reading of Charlotte Mew's strange short story "Passed," which is read as representative of a transitional moment in lesbian literary history.

  19. Pink Is for Girls: Sugar and Spice and Everything Nice--A Case of Single-Sex Education

    Science.gov (United States)

    Martin, Jennifer; Beese, Jane A.

    2016-01-01

    Leaders must know how to use evidence to inform district decisions, particularly as decisions related to learning become standard practice, and provide professional development that builds the organizational capacity needed to support continuous and sustainable district improvement. Collaboration and implementation of a shared vision and mission…

  20. Results of a study of innovative reactors. A report by a working group of the PINK PROGRAMME

    Energy Technology Data Exchange (ETDEWEB)

    Jehee, J N.T. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Swanenburg de Veye, R J [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Bueno de Mesquita, K G [Hoogovens Technical Services, Ijmuiden (Netherlands); Eendebak, B T [Keuring van Electrotechnische Materialen NV, Arnhem (Netherlands); Mheen, W.A.G. van der [Keuring van Electrotechnische Materialen NV, Arnhem (Netherlands); Essen, D van [NUCON Nuclear Technology BV, Amsterdam (Netherlands); Lievense, K [NUCON Nuclear Technology BV, Amsterdam (Netherlands)

    1993-07-01

    In the opinion of the working group, the gas-cooled reactor modules, i.e. the MHTGR and the HTR-M, offer the highest degree of inherent safety. This view is based on the high thermal and chemical stability of coated fuel particles as well as helium coolant and the very high safety margin between core operating conditions and fuel failure limit. Despite the potential flammability of the graphite, it is impossible to envisage any credible accident scenario that could result in a major release of radioactive material to the reactor building or the environment. The HTR-M can be favourably distinguished from the MHTGR regarding its potential to cope with reactivity accidents. Thanks to the continuously refuelled core, inadvertent withdrawal of neutron absorber rods only creates a small increase in reactivity. (orig./HP).