WorldWideScience

Sample records for gina leiss sv

  1. Understanding GINA and How GINA Affects Nurses.

    Science.gov (United States)

    Delk, Kayla L

    2015-11-01

    The Genetic Information Nondiscrimination Act (GINA) is a federal law that became fully effective in 2009 and is intended to prevent employers and health insurers from discriminating against individuals based on their genetic or family history. The article discusses the sections of GINA, what information constitutes genetic information, who enforces GINA, and scenarios in which GINA does not apply. Also discussed are the instances in which an employer may request genetic information from employees, including wellness or genetic monitoring programs. Finally, the article offers a look at how GINA affects nurses who are administering wellness or genetic monitoring programs on behalf of employers. © 2015 The Author(s).

  2. ginas web para el aprendizaje

    Directory of Open Access Journals (Sweden)

    Isabel ÁLVAREZ

    2016-04-01

    Full Text Available Este artículo plantea la construcción de paginas web como vehículo para facilitar la integracion de las tecnologias de la informacion y la comunicacion en el aula para futuros maestros. El término Página Web Coherente enfatiza la utilización de páginas cuyo objetivo principal es promover el aprendizaje siguiendo un proceso colaborativo, una estructura integradora y un contenido educativo.

  3. A summary of the new GINA strategy

    DEFF Research Database (Denmark)

    Reddel, H. K.; Bateman, E. D.; Becker, A.

    2015-01-01

    Over the past 20 years, the Global Initiative for Asthma (GINA) has regularly published and annually updated a global strategy for asthma management and prevention that has formed the basis for many national guidelines. However, uptake of existing guidelines is poor. A major revision of the GINA...... report was published in 2014, and updated in 2015, reflecting an evolving understanding of heterogeneous airways disease, a broader evidence base, increasing interest in targeted treatment, and evidence about effective implementation approaches. During development of the report, the clinical utility...... of recommendations and strategies for their practical implementation were considered in parallel with the scientific evidence. This article provides a summary of key changes in the GINA report, and their rationale. The changes include a revised asthma definition; tools for assessing symptom control and risk factors...

  4. gina web tienda de ultramarinos

    OpenAIRE

    Jauregui Miranda, Mikel

    2014-01-01

    En la actualidad, Internet se ha convertido en una herramienta muy poderosa que utilizan muchas empresas para darse a conocer. Muchas de ellas emplean las redes sociales, blogs o incluso la creación de su propia página web para publicitarse. Además, en estos tiempos de crisis económica es prácticamente imprescindible que una empresa tenga su propia página web, ya que existe una gran competencia entre los comercios y es una buena forma de captar nuevos clientes. Por ello, Ult...

  5. Family physicians' awareness and knowledge of the Genetic Information Non-Discrimination Act (GINA).

    Science.gov (United States)

    Laedtke, Amanda L; O'Neill, Suzanne M; Rubinstein, Wendy S; Vogel, Kristen J

    2012-04-01

    Historically, physicians have expressed concern about their patients' risk of genetic discrimination, which has acted as a barrier to uptake of genetic services. The Genetic Information Nondiscrimination Act of 2008 (GINA) is intended to protect patients against employer and health insurance discrimination. Physicians' awareness and knowledge of GINA has yet to be evaluated. In 2009, we mailed surveys to 1500 randomly selected members of the American Academy of Family Physicians. Questions measured physicians' current knowledge of GINA and their level of concern for genetic discrimination. In total, 401 physicians completed the survey (response rate 26.9%). Approximately half (54.5%) of physicians had no awareness of GINA. Of physicians who reported basic knowledge of GINA, the majority were aware of the protections offered for group health insurance (92.7%), private health insurance (82.9%), and employment (70.7%). Fewer physicians were aware of GINA's limitations regarding life insurance (53.7%) and long-term care insurance (58.8%). Physicians demonstrated highest levels of concern for health insurance, life insurance, and long-term care insurance discrimination, with less concern for employer and family/social discrimination. Level of concern for the risk of genetic discrimination did not correlate significantly with awareness of GINA. Approximately 17 months after GINA was signed into federal law, physicians' knowledge remained limited regarding the existence of this legislation and relevant details. Physicians who are aware of GINA continue to have significant concerns regarding the risk of genetic discrimination. This study reveals the need to further educate physicians about the existence of GINA and the protections offered.

  6. gina web amb gestor de continguts

    OpenAIRE

    Castells Gonzalbo, Pau

    2014-01-01

    En aquest informe es detalla l'anàlisi previ i posterior a la implementació del projecte, que consta del disseny i implementació d'una eina per gestionar el contingut d'una pàgina web, concretament una web orientada a músics. En este informe se detalla el análisis previo y posterior a la implementación del proyecto, que consta del diseño e implementación de una herramienta para gestionar el contenido de una página web, concretamente una web orientada a músicos.

  7. The GINA asthma strategy report: what's new for primary care?

    Science.gov (United States)

    Reddel, Helen K; Levy, Mark L

    2015-07-30

    The Global Initiative for Asthma (GINA) was established in 1993 by the World Health Organization and National Heart Lung and Blood Institute to develop a global strategy for managing and preventing asthma. GINA reports, now funded independently through the sale of GINA products, have provided the foundation for many national guidelines. They are prepared by international experts from primary, secondary and tertiary care, and are annually updated following a review of evidence. In 2014, a major revision of the GINA report was published, that took into account advances in evidence not only about asthma and its treatment, but also about how to improve implementation of evidence-based recommendations in clinical practice. This paper summarises key changes relevant to primary care in the new GINA report. A noticeable difference is the report's radically different approach, now clinically-focussed, with multiple practical tools and flow charts to improve its utility for busy frontline clinicians. Key changes in recommendations include a new, diagnosis-centred definition of asthma; more detail about how to assess current symptom control and future risk; a comprehensive approach to tailoring treatment for individual patients; expanded indications for commencing inhaled corticosteroids; new recommendations for written asthma action plans; a new chapter on diagnosis and initial treatment of patients with asthma-COPD overlap syndrome; and a revised approach to diagnosing asthma in preschool children. The 2014 GINA report (further updated in 2015) moved away from a 'textbook' approach to provide clinicians with up-to-date evidence about strategies to control symptoms and minimise asthma risk, in a practical, practice-centred format.

  8. gina

    OpenAIRE

    Gonçalves, Sofia

    2009-01-01

    No território de passagem ou confluência do objecto ao sistema, o artigo procura circunscrever os aspectos essenciais da mudança de paradigma no design de comunicação operada pela cultura digital, utilizando o livro e a página como modelos exemplares de reflexo das alterações ocorridas. Do texto ao hipertexto, do plano ao “sítio”, estes artefactos constroem um possível “ecotone” para uma continuidade negociada, i.e. o local onde se encontram “tradicional” e “novo”, história e contempora...

  9. SvABA

    DEFF Research Database (Denmark)

    Wala, Jeremiah A; Bandopadhayay, Pratiti; Greenwald, Noah

    2018-01-01

    Structural variants (SVs), including small insertion and deletion variants (indels), are challenging to detect through standard alignment-based variant calling methods. Sequence assembly offers a powerful approach to identifying SVs, but is difficult to apply at scale genome-wide for SV detection...... due to its computational complexity and the difficulty of extracting SVs from assembly contigs. We describe SvABA, an efficient and accurate method for detecting SVs from short-read sequencing data using genome-wide local assembly with low memory and computing requirements. We evaluated Sv...... complex somatic rearrangements with chains of short (applied SvABA to 344 cancer genomes from 11 cancer types and found that short templated-sequence insertions occur in ∼4% of all somatic rearrangements. Finally, we...

  10. La página web del Senado de España: Indignado

    OpenAIRE

    Luján Mora, Sergio

    2012-01-01

    La página web del Senado de España incumple la legislación española en materia de accesibilidad web. La página web del Senado debería ser accesible desde principios de 2012, pero todavía no lo es (mayo 2012).

  11. The Effectiveness of Social Media as a Marketing Communication Tactic : Case Gina Tricot Ltd

    OpenAIRE

    Kallio, Kira

    2015-01-01

    The aim of this thesis was to discover whether social media is an effective marketing communication tactic for Gina Tricot or not. The objective was to find out how customers experience Gina Tricot’s marketing and social media activities and whether social media accounts are able to serve customers as aimed. The case company Gina Tricot is presented in the introduction chapter. The theoretical study focuses on social media marketing and on the transition from traditional outbound marketing to...

  12. Análisis de usabilidad de la página web de la Fundació CIM

    OpenAIRE

    Rosendo Pulido, Miguel Angel

    2016-01-01

    Análisis de usabilidad de la página web de la Fundació CIM. Engloba todo el conocimiento sobre la organización, la página web actual y usabilidad necesario para entender el proceso. Incluye un análisis de usabilidad completo de la página web, pruebas de usabilidad y una propuesta de mejora.

  13. 77 FR 5396 - Recordkeeping and Reporting Requirements Under Title VII, the ADA, and GINA

    Science.gov (United States)

    2012-02-03

    ... 2008 (GINA) into law. Title II of GINA protects job applicants, current and former employees, labor... creation of any documents or impose any reporting requirements. It imposes the same record retention... reports or the creation of new documents; it merely requires retention of documents that the employer has...

  14. 76 FR 57013 - Recordkeeping and Reporting Requirements Under Title VII, the ADA, and GINA

    Science.gov (United States)

    2011-09-15

    ... EQUAL EMPLOYMENT OPPORTUNITY COMMISSION 29 CFR Part 1602 RIN 3046-AA89 Recordkeeping and Reporting Requirements Under Title VII, the ADA, and GINA AGENCY: Equal Employment Opportunity Commission. ACTION... under title VII, the ADA, and GINA. (76 FR 31892, June 2, 2011). No requests to present oral testimony...

  15. 76 FR 31892 - Recordkeeping and Reporting Requirements Under Title VII, the ADA, and GINA

    Science.gov (United States)

    2011-06-02

    ... Requirements Under Title VII, the ADA, and GINA AGENCY: Equal Employment Opportunity Commission. ACTION: Notice... Civil Rights Act of 1964 (Title VII) and the Americans with Disabilities Act (ADA) to entities covered... the same record retention requirements under GINA that are imposed under Title VII and the ADA, i.e...

  16. 76 FR 79065 - Recordkeeping and Reporting Requirements Under Title VII, the ADA and GINA

    Science.gov (United States)

    2011-12-21

    ... Reporting Requirements Under Title VII, the ADA and GINA CFR Correction In Title 29 of the Code of Federal... title VII or section 107 of the ADA'' and add in their place the words ``section 709(c) of title VII, section 107 of the ADA, or section 207(a) of GINA'' wherever they appear in the following sections...

  17. ER Operations Installation of Three FLUTe Soil-Vapor Monitoring Wells (MWL-SV03 MWL-SV04 and MWL-SV05) at the Mixed Waste Landfill.

    Energy Technology Data Exchange (ETDEWEB)

    Copland, John Robin [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2014-09-01

    This installation report describes the May through July 2014 drilling activities performed for the installation of three multi-port soil-vapor monitoring wells (MWL-SV03, MWL-SV04, and MWL-SV05) at the Mixed Waste Landfill (MWL), which is located at Sandia National Laboratories, New Mexico (SNL/NM). SNL/NM is managed and operated by Sandia Corporation (Sandia), a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy (DOE)/National Nuclear Security Administration. The MWL is designated as Solid Waste Management Unit (SWMU) 76 and is located in Technical Area (TA) III (Figure 1-1). The locations of the three soil-vapor monitoring wells (MWL-SV03, MWL-SV04, and MWL-SV05) are shown in Figure 1-2

  18. Título da página electrónica: Multitudes Web

    Directory of Open Access Journals (Sweden)

    Pedro Araújo

    2012-10-01

    Full Text Available Página de uma revista política, cultural e artística votada à análise de todas as formas de dominação e dos movimentos sociais visando a emancipação social. Além da Revue Multitudes, disponível online, encontram-se igualmente na página análises da actualidade, um espaço de debate, complementos à revista com diversas contribuições internacionais e um arquivo onde é possível aceder às revistas Futur Antérieur e Alice, Revue Critique du Temps. A revista Multitudes integra a rede Eurozine.

  19. Implementation of GINA guidelines in Ho Chi Minh City: a model for Viet Nam.

    Science.gov (United States)

    Tho, N V; Loan, H T H; Thao, N T P; Dung, N T T; Lan, L T T

    2012-12-21

    The Global Initiative for Asthma (GINA) guidelines have not been implemented effectively in primary care settings in Viet Nam. To estimate the proportion of patients with controlled asthma and the direct health care costs of managing asthma according to GINA guidelines at four out-patient clinics in Ho Chi Minh City (HCMC), Viet Nam. One hundred and six patients with asthma were treated and followed up according to GINA guidelines for 12 months. Clinical and pulmonary function responses and direct health care costs were evaluated every 3 months during the study. The proportion of patients with controlled asthma rose from 1.0% at the start of the study to 36.8% by the end of the study (P Viet Nam, improved asthma control with modest direct health care costs.

  20. GINA--a polarized neutron reflectometer at the Budapest Neutron Centre.

    Science.gov (United States)

    Bottyán, L; Merkel, D G; Nagy, B; Füzi, J; Sajti, Sz; Deák, L; Endrőczi, G; Petrenko, A V; Major, J

    2013-01-01

    The setup, capabilities, and operation parameters of the neutron reflectometer GINA, the recently installed "Grazing Incidence Neutron Apparatus" at the Budapest Neutron Centre, are introduced. GINA, a dance-floor-type, constant-energy, angle-dispersive reflectometer is equipped with a 2D position-sensitive detector to study specular and off-specular scattering. Wavelength options between 3.2 and 5.7 Å are available for unpolarized and polarized neutrons. Spin polarization and analysis are achieved by magnetized transmission supermirrors and radio-frequency adiabatic spin flippers. As a result of vertical focusing by a five-element pyrolytic graphite monochromator, the reflected intensity from a 20 × 20 mm(2) sample has been doubled. GINA is dedicated to studies of magnetic films and heterostructures, but unpolarized options for non-magnetic films, membranes, and other surfaces are also provided. Shortly after its startup, reflectivity values as low as 3 × 10(-5) have been measured by the instrument. The instrument capabilities are demonstrated by a non-polarized and a polarized reflectivity experiment on a Si wafer and on a magnetic film of [(62)Ni/(nat)Ni](5) isotope-periodic layer composition. The facility is now open for the international user community. Its further development is underway establishing new sample environment options and spin analysis of off-specularly scattered radiation as well as further decreasing the background.

  1. GINA-A polarized neutron reflectometer at the Budapest Neutron Centre

    Energy Technology Data Exchange (ETDEWEB)

    Bottyan, L.; Merkel, D. G.; Nagy, B.; Sajti, Sz.; Deak, L.; Endroczi, G. [Wigner RCP, RMKI, H-1525 Budapest, P.O. Box 49 (Hungary); Fuezi, J. [Wigner RCP, SZFKI, H-1525 Budapest, P.O. Box 49 (Hungary); University of Pecs, Pollack Mihaly Faculty of Engineering and Information Technology, H-7602 Pecs, P.O. Box 219 (Hungary); Petrenko, A. V. [Frank Laboratory of Neutron Physics, JINR, Joliot-Curie 6, Dubna, 141980 (Russian Federation); Major, J. [Wigner RCP, RMKI, H-1525 Budapest, P.O. Box 49 (Hungary); Max-Planck-Institut fuer Intelligente Systeme (formerly Max-Planck-Institut fuer Metallforschung), Heisenbergstr. 3, D-70569 Stuttgart (Germany)

    2013-01-15

    The setup, capabilities, and operation parameters of the neutron reflectometer GINA, the recently installed 'Grazing Incidence Neutron Apparatus' at the Budapest Neutron Centre, are introduced. GINA, a dance-floor-type, constant-energy, angle-dispersive reflectometer is equipped with a 2D position-sensitive detector to study specular and off-specular scattering. Wavelength options between 3.2 and 5.7 A are available for unpolarized and polarized neutrons. Spin polarization and analysis are achieved by magnetized transmission supermirrors and radio-frequency adiabatic spin flippers. As a result of vertical focusing by a five-element pyrolytic graphite monochromator, the reflected intensity from a 20 Multiplication-Sign 20 mm{sup 2} sample has been doubled. GINA is dedicated to studies of magnetic films and heterostructures, but unpolarized options for non-magnetic films, membranes, and other surfaces are also provided. Shortly after its startup, reflectivity values as low as 3 Multiplication-Sign 10{sup -5} have been measured by the instrument. The instrument capabilities are demonstrated by a non-polarized and a polarized reflectivity experiment on a Si wafer and on a magnetic film of [{sup 62}Ni/{sup nat}Ni]{sub 5} isotope-periodic layer composition. The facility is now open for the international user community. Its further development is underway establishing new sample environment options and spin analysis of off-specularly scattered radiation as well as further decreasing the background.

  2. Título da página electrónica: Pamela Oliver Homepage

    Directory of Open Access Journals (Sweden)

    José Manuel Mendes

    2012-10-01

    Full Text Available Esta é a página pessoal de Pamela Oliver, professora do Departamento de Sociologia na Universidade de Wisconsin-Madison, nos Estados Unidos. A autora é especialista no estudo dos movimentos sociais e no protesto social, tanto numa vertente quantitativa como qualitativa. Realço nesta página a secção intitulada Protest Dynamics (Dinâmicas de Protesto. Entre os vários artigos disponibilizados, são de especial importância os que se atêm à análise do papel dos media na construção dos aconteciment...

  3. Random integration of SV40 in SV40-transformed, immortalized human fibroblasts.

    Science.gov (United States)

    Hara, H; Kaji, H

    1987-02-01

    We have studied the relationship between immortalization of SV40-transformed human embryonic fibroblasts and their SV40 integration sites. From several independently transformed cell pools, we have isolated clones which do not harbor unintegrated SV40 DNA. We have analysed whole-cell DNA from these clones, using the Southern blot method. Our results suggest that no specific integration sites in the cellular genome exist which are a prerequisite for the immortalization process. Although some integration sites were found to be predominant in pre-crisis clones, they could not be detected in the post-crisis clones. This suggests that none of these predominating sites is selected for during the crisis period.

  4. Is the standard dose rate for de-contamination, 0.23 mc-Sv/hr, truly 1 mSv/year?

    International Nuclear Information System (INIS)

    Furuta, Sadaaki

    2014-01-01

    Examined is the validity of the standard dose rates in the title, which have been additionally defined by Ministry of the Environment (ME) to the measures of Fukushima Nuclear Power Plant Accident. The standard 0.23 mc-Sv/hr is the sum of natural ambient dose rate 0.04 mc-Sv/hr in average of Japan as measured with NaI scintillation surveymeter, plus additional accidental exposure dose 1 mSv/y, which is defined equivalent to 0.19 mc-Sv/hr. Here, the equation of addition 0.04+0.19 mc-Sv/hr is not exactly correct because the unit of each dose value has different means as follows. The natural dose is derived from the value 5.8 mc-R/hr (0.038 mc-Sv/hr, effective dose) of the average national natural dose data (2011) of Ministry of Education, Culture, Sports, Science and Technology. However, if the measurement is done with the surveymeter which practically gives 1 cm dose equivalent, the rate is calculated to be 0.06 mc-Sv/hr. When the Fukushima prefectural natural dose rate 6.4 mc-R/hr is employed, the calculation gives 0.07 mc-Sv/hr. ME defines the additional doses to be 0.19 mc-Sv/hr and 1 mSv/y, which are derived from the calculation: (0.19 mc-Sv/hr x 8 hr outdoor + 0.19 mc-Sv/hr x 0.4 indoor, shielded) x 365 d/y= 1 mSv/y. The dose is assumed to be measurable with the surveymeter (1 cm dose equivalent) and thereby calculation, if the source is mainly "1"3"4Cs and "1"3"7Cs, gives the effective dose of 0.32 mc-Sv/hr to be managed by the meter. As this, the effective dose unit and 1 cm equivalent dose unit are used for calculation of the administrative standard dose rate of 1 mSv/y, which should be recognized by both radiological experts and administration for the explanation to general public. (T.T.)

  5. Simvastatin (SV) metabolites in mouse tissues

    International Nuclear Information System (INIS)

    Duncan, C.A.; Vickers, S.

    1990-01-01

    SV, a semisynthetic analog of lovastatin, is hydrolyzed in vivo to its hydroxy acid (SVA), a potent inhibitor of HMG CoA reductase (HR). Thus SV lowers plasma cholesterol. SV is a substrate for mixed function oxidases whereas SVA undergoes lactonization and β-oxidation. Male CD-1 mice were dosed orally with a combination of ( 14 C)SV and ( 3 H)SVA at 25 mg/kg of each, bled and killed at 0.5, 2 and 4 hours. Labeled SV, SVA, 6'exomethylene SV (I), 6'CH 2 OH-SV (II), 6'COOH-SV (III) and a β-oxidized metabolite (IV) were assayed in liver, bile, kidneys, testes and plasma by RIDA. Levels of potential and active HR inhibitors in liver were 10 to 40 fold higher than in other tissues. II and III, in which the configuration at 6' is inverted, may be 2 metabolites of I. Metabolites I-III are inhibitors of HR in their hydroxy acid forms. Qualitatively ( 14 C)SV and ( 3 H)SVA were metabolized similarly (consistent with their proposed interconversion). However 3 H-SVA, I-III (including hydroxy acid forms) achieved higher concentrations than corresponding 14 C compounds (except in gall bladder bile). Major radioactive metabolites in liver were II-IV (including hydroxy acid forms). These metabolites have also been reported in rat tissues. In bile a large fraction of either label was unidentified polar metabolites. The presence of IV indicated that mice (like rats) are not good models for SV metabolism in man

  6. One-step simultaneous detection of Ureaplasma parvum and genotypes SV1, SV3 and SV6 from clinical samples using PlexPCR technology.

    Science.gov (United States)

    Payne, M S; Furfaro, L L; Tucker, R; Tan, L Y; Mokany, E

    2017-08-01

    Ureaplasma spp. are associated with preterm birth. In recent times, it has become apparent that Ureaplasma parvum, but not Ureaplasma urealyticum, is of most relevance. We recently demonstrated this in Australian pregnant women and using high-resolution melt (HRM) PCR, further showed that U. parvum genotype SV6 was of particular significance. However, our assay was unable to identify multiple genotypes in the same sample, required a separate species-level qPCR for low titre samples and was not ideal for diagnostic laboratories due to the nature of HRM PCR result interpretation. Consequently, our current study developed a novel, one-step PlexPCR assay capable of detecting U. parvum and genotypes SV1, SV3 and SV6 in a single reaction directly from clinical samples. We then validated this using vaginal swab DNA from our Australian cohort of pregnant women. The PlexPCR was highly sensitive, detecting all targets to between 0.4 × 10 -5  ng DNA (SV3) and 0.4 × 10 -6  ng DNA (U. parvum, SV1 and SV6). Compared to our HRM PCR, the PlexPCR defined genotype distribution in all seven cases previously reported as 'mixed', and detected another eight cases where multiple genotypes (two) were present in samples previously reported as single genotypes using HRM PCR. Ureaplasma spp. have been associated with prematurity for decades, however, only a minority of studies have examined this beyond the genus level. In those that have, Ureaplasma parvum has been strongly associated with preterm birth. We recently demonstrated this in Australian women and further showed that U. parvum genotype SV6 was of particular significance. Our PlexPCR assay allows rapid detection and concurrent genotyping of U. parvum in clinical samples and may be of particular interest to obstetricians, particularly those caring for women at a high risk of preterm birth, and any other disease phenotypes where U. parvum is of interest. © 2017 The Authors. Letters in Applied Microbiology published by John

  7. The Genetic Information Nondiscrimination Act (GINA): A Civil Rights Victory

    Science.gov (United States)

    Petruniak, Mark; Krokosky, Alyson; Terry, Sharon F.

    2011-01-01

    This article discusses the Genetic Information Nondiscrimination Act (GINA) which President George W. Bush officially signed in 2008. The law prohibits employers from making adverse employment decisions based on a person's genetic information, including family health history. It also forbids insurance companies from discriminating against…

  8. Commentaar op art. 563 Sv

    NARCIS (Netherlands)

    Knoops, G.G.J.; van Laanen, F.; Melai, A.L.; Groenhuijsen, M.S.

    2004-01-01

    Schrijvers verschaffen een wetenschappelijk commentaar op art. 563 Sv, betreffende de tenuitvoerlegging van andere straffen dan de bij art. 562 Sv bedoelde straffen ingeval van een veroordeelde wiens geestvermogens ziekelijk zijn gestoord.

  9. Protecting posted genes: social networking and the limits of GINA.

    Science.gov (United States)

    Soo-Jin Lee, Sandra; Borgelt, Emily

    2014-01-01

    The combination of decreased genotyping costs and prolific social media use is fueling a personal genetic testing industry in which consumers purchase and interact with genetic risk information online. Consumers and their genetic risk profiles are protected in some respects by the 2008 federal Genetic Information Nondiscrimination Act (GINA), which forbids the discriminatory use of genetic information by employers and health insurers; however, practical and technical limitations undermine its enforceability, given the everyday practices of online social networking and its impact on the workplace. In the Web 2.0 era, employers in most states can legally search about job candidates and employees online, probing social networking sites for personal information that might bear on hiring and employment decisions. We examine GINA's protections for online sharing of genetic information as well as its limitations, and propose policy recommendations to address current gaps that leave employees' genetic information vulnerable in a Web-based world.

  10. Persistencia de modelos de diseño gráfico en las páginas web

    Directory of Open Access Journals (Sweden)

    Mª Luisa García Guardia

    2012-04-01

    Full Text Available El objetivo del artículo es conocer si realmente existe un modelo que de forma repetitiva es utilizado en la creación de páginas desde el campo del diseño gráfico. Para ello se ha desarrollado un estudio descriptivo funcional utilizando las páginas de inicio de quince sitios webs; un  estudio sobre la valoración de la correlación entre profesionales y estudiantes, partiendo de la hipótesis de que existen diferencias en dicha valoración; y una comparativa de ambos estudios. Los resultados obtenidos  muestran que la “página ideal” sería aquella: con el buscador centrado, copyright, el logotipo centrado, la ubicación de la botonera centrada, los tipos botoneras verticales y con animación.

  11. ANÁLISE DA TRADUÇÃO PARA O ESPANHOL DA PÁGINA WEB DO PORTAL DO MINISTÉRIO BRASILEIRO DE TURISMO

    Directory of Open Access Journals (Sweden)

    Antonio Messias Nogueira da Silva

    2011-11-01

    Full Text Available El objetivo principal de este estudio es realizar un análisis traductológico de  la página web de publicidad turística del Ministerio Brasileño de Turismo, escrita originalmente en portugués de Brasil y traducida al español. En especial, realizaremos un estudio de los tipos de errores traductológicos que comete el traductor de esa página en relación al par de lenguas portugués-español y la especialidad traductológica. Además analizaremos las estrategias utilizadas por el traductor de esta página de publicidad, teniendo en cuenta el aspecto verbal y no verbal de la página web.

  12. Biological activity of SV40 DNA

    International Nuclear Information System (INIS)

    Abrahams, P.J.

    1978-01-01

    This thesis deals with a study on the biological activity of SV40 DNA. The transforming activity of SV40 DNA and DNA fragments is investigated in order to define as precisely as possible the area of the viral genome that is involved in the transformation. The infectivity of SV40 DNA is used to study the defective repair mechanisms of radiation damages of human xeroderma pigmentosum cells. (C.F.)

  13. Tallinna tüdruk Gina liigutab Räniorus sadu miljoneid dollareid / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2007-01-01

    Riskikapitali BlueRun Ventures looja ning turundusjuhi Gina Baumani sõnul investeerib ettevõte vaid kõige parematesse ideedesse üle maailma, samuti aitab Bauman kohapeal tegevust alustavaid eestlasi

  14. Inventario etnográfico de las parteras de Sierra Mágina: Aproximación a su relación con las Matronas Ethnographic inventory of the traditional midwives of mountain Mágina: approach to their relationship with the titled midwives

    Directory of Open Access Journals (Sweden)

    Manuel Linares-Abad

    2005-03-01

    Full Text Available Introducción. Los cuidados a las mujeres durante el parto han sido tradicionalmente prestados por mujeres. La partera tradicional ha jugado un papel decisivo en la asistencia al nacimiento en las comunidades donde vivieron en tanto que se producía la institucionalización de las profesiones sanitarias. Pretendemos por tanto, visibilizar la identidad de unas mujeres de una comarca concreta, Sierra Mágina (Jaén y la relación que mantuvieron con matronas tituladas contribuyendo a la historia de las gestoras del nacimiento desde la categoría de análisis de género. Participantes y método. Se trata de un microanálisis realizado entre los meses de Enero y Agosto de 2004 con técnicas de investigación etnográfica. El ámbito de estudio es la comarca de Sierra Mágina y el objeto de estudio las parteras tradicionales. Resultados. Hemos rescatado de la memoria colectiva y de las propias protagonistas los nombres o apodos de 41 parteras que ejercieron en Sierra Mágina desde principio hasta mediados del siglo XX. Conclusiones. En Mágina ha habido un gran número de parteras sin formación académica que han prestado sus servicios a la comunidad en la primera mitad del siglo XX. Hubo variedad de relaciones con las matronas, influenciada por diversos factores.Introduction. The cares to the women during the childbirth have been traditionally borrowed for women. The traditional midwives have played a decisive paper in the attendance to the birth in the communities where they lived as long as the installation of the sanitary professions. We seek therefore, to take out to the light the identity of a women of a concrete district, Mountain Mágina (Jaén and the relationship that they maintained with titled midwives contributing to the history of the agents of the birth from the category of gender analysis. Method. It’s a microanalysis carried out between the months of January and August of 2004 with technical of ethnographic research. The study

  15. Improvement of bovine ß-lactoglobulin production and secretion by Lactococcus lactis

    Directory of Open Access Journals (Sweden)

    S. Nouaille

    2005-03-01

    Full Text Available The stabilizing effects of staphylococcal nuclease (Nuc and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS on the production of a model food allergen, bovine ß-lactoglobulin (BLG, in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively. Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml (~2-fold higher than Nuc-BLG. In conclusion, the fusions described here led to the improvement of the production and secretion of BLG. These tools will be used to modulate the immune response against BLG via delivery of recombinant lactococci at the mucosal level, in a mouse model of cow's milk allergy.

  16. Título da página electrónica: Z Net – A Community of People Commited to Social Change

    Directory of Open Access Journals (Sweden)

    José Manuel Mendes

    2012-10-01

    Full Text Available Esta página electrónica inclui-se no projecto Z Communications – The Spirit of Resistance Lives, uma iniciativa lançada por Michael Albert e Lydia Sargent que propõe uma visão crítica e alternativa ao sistema capitalista mundial. Entre os vários colaboradores desta página contam-se, entre outros, Noam Chomsky, Arundhati Roy e Vandana Shiva, com muitos documentos próprios disponíveis. Entre as várias secções que constituem esta página, são de salientar as seguintes: a revista Z Magazine com a...

  17. As páginas de história

    Directory of Open Access Journals (Sweden)

    Höfling Maria Arlete Zülzke

    2003-01-01

    Full Text Available Produzimos um material para o estudo da localidade num Atlas Municipal Escolar, objetivando despertar a curiosidade e o gosto pelo conhecimento da história local. Destacamos nas páginas de história do Atlas de Rio Claro o processo de ocupação, povoamento, doação de sesmarias, desmembramento em fazendas, a cana-de-açúcar, o café, o escravo, o imigrante e as mudanças que ocorreram em Rio Claro até o início do século XX.

  18. Título da página electrónica: International Confederation of Free Trade Unions

    Directory of Open Access Journals (Sweden)

    Hermes Augusto Costa

    2012-10-01

    Full Text Available Embora se trate da maior organização sindical mundial e tenha completado já meio século de vida, só a partir de 1996 é que a Confederação Internacional dos Sindicatos Livres (CISL despertou para o sindicalismo online. Tal como sucede com a página da OIT, parece excessivo o número de itens com que se deparam os cibernautas que entram na página. Em todo o caso, a igualdade no trabalho, os direitos sindicais, as empresas multinacionais, a globalização e seus impactos sobre o trabalho, o comérci...

  19. iDESWEB: Ejercicio: JavaScript: Una página web con un poco de código (encuesta de valoración)

    OpenAIRE

    Luján Mora, Sergio

    2013-01-01

    Solución del ejercicio "JavaScript: Una página web con un poco de código (encuesta de valoración)" del curso MOOC iDESWEB, Introducción al desarrollo web. Página web del curso: http://idesweb.es/

  20. Título da página electrónica: The Children and Armed Conflict Unit

    Directory of Open Access Journals (Sweden)

    Gilberto C. Oliveira

    2012-10-01

    Full Text Available Esta página refere‑se a um projecto desenvolvido em conjunto pela The Children’s Legal Centre, uma organização não‑governamental britânica, e a Universidade de Essex. O objectivo da página é tornar público o impacto dos conflitos armados sobre as crianças. Nesse sentido, são divulgadas, de forma regular e actualizada, as notícias sobre conflitos, violação dos direitos humanos e questões humanitárias que afectam as crianças em todo o mundo. Essas notícias são arquivadas desde 1999 e os casos m...

  1. Oltar Sv. Jeronima u crkvi Sv. Šime u Zadru i radionica Bettamelli

    Directory of Open Access Journals (Sweden)

    Bojan Goja

    2012-12-01

    Full Text Available U radu se na temelju arhivskih podataka govori o oltaru Sv. Jeronima u crkvi Sv. Šime u Zadru. Od ranije se zna da je oltar dala podići i o njegovu se uređenju brinula bratovština hrvatskih i albanskih vojnika (Croatti a cavallo i Soldati Albanesi u službi Mletačke Republike, osnovana 1675. godine u Zadru. Novim je arhivskim istraživanjima utvrđeno da je dana 26. rujna 1694. godine bratovština odobrila izdatak u iznosu od 200 srebrnih dukata, namijenjenih venecijanskim klesarima braći Bettamelli kao predujam za izradu oltara te da su radovi na njegovu podizanju započeti u travnju 1696. godine. Na temelju zapisa o gradnji oltara iznijeta je pretpostavka da se grb na istočnom pilastru stipesa oltara, ranije povezivan s predstavnicima obitelji Civran, odnosi na zadarskog plemića i istaknutog zapovjednika u mletačkoj vojsci, Šimuna Fanfognu (Zadar, 7. travnja 1663. - Lendinara, 6. ožujka 1707. koji je obavljao i dužnost prokuratora oltara. Brojni radovi na uređenju oltara i kapele Sv. Jeronima obavljani su i tijekom čitavog 18. stoljeća, a na njima su bili uposleni brojni mjesni majstori različitih struka: graditelji Antonio Piovesana (1742. i Antonio Bernardini (1789., oltarist Girolamo Picco (1756., marangon Domenico Tomaselli (1743., kovač Antonelli (1744. te zlatari Zorzi Cullisich (1738., Nicolò Giurovich (1752. i Giuseppe Rado (1755.. Donose se još neke zanimljivosti vezane uz uređenje oltara i djelovanje bratovštine Sv. Jeronima.

  2. Título da página: Queer Resources Directory

    Directory of Open Access Journals (Sweden)

    Ana Cristina Santos

    2012-10-01

    Full Text Available Embora a apresentação visual desta página não disponha de características imediatamente apelativas, os conteúdos disponibilizados constituem um importante recurso para quem se interesse por temas queer. Neste directório encontram-se mais de vinte e cinco mil ficheiros cruzando a temática queer com questões relacionadas com activismo, cultura, família, história, juventude, legislação, media, política, religião, trabalho, saúde, etc.

  3. Svømmehaller og krav til energieffektivitet

    OpenAIRE

    Øen, Martin Nerhus

    2010-01-01

    Svømmehaller er en spesiell bygningstype, som med høy innendørs temperatur og luftfuktighet, skiller seg fra andre bygninger som boliger og kontorbygg. Den høye innetemperaturen, forbruket av varmtvann, og fordampning fra svømmebassenget fører til et stort energiforbruk. Svømmehaller er ikke gitt egne krav i Teknisk forskrift til plan- og bygningsloven (TEK), til tross for at energiforbruket kan være tre ganger høyere enn kravene gitt for idrettshaller. Målet med denne rapporten er å finne fo...

  4. Levetiracetam reverses synaptic deficits produced by overexpression of SV2A.

    Directory of Open Access Journals (Sweden)

    Amy Nowack

    Full Text Available Levetiracetam is an FDA-approved drug used to treat epilepsy and other disorders of the nervous system. Although it is known that levetiracetam binds the synaptic vesicle protein SV2A, how drug binding affects synaptic functioning remains unknown. Here we report that levetiracetam reverses the effects of excess SV2A in autaptic hippocampal neurons. Expression of an SV2A-EGFP fusion protein produced a ∼1.5-fold increase in synaptic levels of SV2, and resulted in reduced synaptic release probability. The overexpression phenotype parallels that seen in neurons from SV2 knockout mice, which experience severe seizures. Overexpression of SV2A also increased synaptic levels of the calcium-sensor protein synaptotagmin, an SV2-binding protein whose stability and trafficking are regulated by SV2. Treatment with levetiracetam rescued normal neurotransmission and restored normal levels of SV2 and synaptotagmin at the synapse. These results indicate that changes in SV2 expression in either direction impact neurotransmission, and suggest that levetiracetam may modulate SV2 protein interactions.

  5. Utilización de una página web para la enseñanza del Método Pilates

    OpenAIRE

    Camblor, Ezequiel; Jócano, Érica

    2011-01-01

    En el presente trabajo se describe la utilización de una página web cómo herramienta que pretende emular los procesos involucrados en las decisiones de construcción de una clase de Pilates y que tiene como objetivos proporcionar: instancias prácticas de toma de decisiones y herramientas de nivelación de conocimientos, además de evaluar conocimientos adquiridos. La navegación por la página permite: contribuir al aprendizaje por descubrimiento, poner en juego la intuición, el pensamiento imagin...

  6. Título da Página Electrónica: Directorate for Education of the Organization for Economic Co-Operation and Development

    Directory of Open Access Journals (Sweden)

    Alfredo Campos

    2012-10-01

    Full Text Available Esta é a página do Directório para a Educação da Organização para a Cooperação e Desenvolvimento Económico (OCDE. Na página de rosto, além de notícias e destaques, podemos encontrar ligações imediatas para as últimas publicações, bem como as ligações às suas várias secções: Educação Escolar e Pré-escolar; Educação Superior e de Adultos; Educação, Economia e Sociedade; Gestão de Investigação e Conhecimento; bem como ao tópico de Ciência e Inovação. Logo nesta página de rosto encontra-se o ace...

  7. Título da página: European Transgender Network

    Directory of Open Access Journals (Sweden)

    Ana Cristina Santos

    2012-10-01

    Full Text Available A página desta federação europeia de grupos e associações transgénero reúne informações detalhadas sobre a situação do transgenderism em muitos países europeus, disponibilizando dados actualizados sobre as principais reivindicações e medidas políticas levadas a cabo por activistas e governos. Para além dos documentos fundadores desta rede, é ainda facilitada informação acerca das principais campanhas desenvolvidas, bem como ligações para grupos e associações nacionais.

  8. SV-AUTOPILOT: optimized, automated construction of structural variation discovery and benchmarking pipelines.

    Science.gov (United States)

    Leung, Wai Yi; Marschall, Tobias; Paudel, Yogesh; Falquet, Laurent; Mei, Hailiang; Schönhuth, Alexander; Maoz Moss, Tiffanie Yael

    2015-03-25

    Many tools exist to predict structural variants (SVs), utilizing a variety of algorithms. However, they have largely been developed and tested on human germline or somatic (e.g. cancer) variation. It seems appropriate to exploit this wealth of technology available for humans also for other species. Objectives of this work included: a) Creating an automated, standardized pipeline for SV prediction. b) Identifying the best tool(s) for SV prediction through benchmarking. c) Providing a statistically sound method for merging SV calls. The SV-AUTOPILOT meta-tool platform is an automated pipeline for standardization of SV prediction and SV tool development in paired-end next-generation sequencing (NGS) analysis. SV-AUTOPILOT comes in the form of a virtual machine, which includes all datasets, tools and algorithms presented here. The virtual machine easily allows one to add, replace and update genomes, SV callers and post-processing routines and therefore provides an easy, out-of-the-box environment for complex SV discovery tasks. SV-AUTOPILOT was used to make a direct comparison between 7 popular SV tools on the Arabidopsis thaliana genome using the Landsberg (Ler) ecotype as a standardized dataset. Recall and precision measurements suggest that Pindel and Clever were the most adaptable to this dataset across all size ranges while Delly performed well for SVs larger than 250 nucleotides. A novel, statistically-sound merging process, which can control the false discovery rate, reduced the false positive rate on the Arabidopsis benchmark dataset used here by >60%. SV-AUTOPILOT provides a meta-tool platform for future SV tool development and the benchmarking of tools on other genomes using a standardized pipeline. It optimizes detection of SVs in non-human genomes using statistically robust merging. The benchmarking in this study has demonstrated the power of 7 different SV tools for analyzing different size classes and types of structural variants. The optional merge

  9. Lampião da esquina: lutas feministas nas páginas do "Jornal Gay", luzes em tempos sombrios (Brasil, 1978-1981)

    OpenAIRE

    Silva, Daniel Henrique de Oliveira

    2016-01-01

    Quais as representações do feminino construídas e veiculadas nas páginas de um jornal escrito por editores gays? Tal questionamento orienta uma pesquisa que busca entender como homossexuais masculinos, sujeitos inferiorizados e estigmatizados socialmente, deram visibilidade para outro grupo marginalizado, nesse caso as mulheres, nas páginas de um jornal considerado marginal, o Lampião da Esquina, que surgiu em 1978, anos finais da ditadura civil – militar. Nessa pesquisa, buscou-se contextual...

  10. Stimulation of NADH-dependent microsomal DNA strand cleavage by rifamycin SV.

    Science.gov (United States)

    Kukiełka, E; Cederbaum, A I

    1995-04-15

    Rifamycin SV is an antibiotic anti-bacterial agent used in the treatment of tuberculosis. This drug can autoxidize, especially in the presence of metals, and generate reactive oxygen species. A previous study indicated that rifamycin SV can increase NADH-dependent microsomal production of reactive oxygen species. The current study evaluated the ability of rifamycin SV to interact with iron and increase microsomal production of hydroxyl radical, as detected by conversion of supercoiled plasmid DNA into the relaxed open circular state. The plasmid used was pBluescript II KS(-), and the forms of DNA were separated by agarose-gel electrophoresis. Incubation of rat liver microsomes with plasmid plus NADH plus ferric-ATP caused DNA strand cleavage. The addition of rifamycin SV produced a time- and concentration-dependent increase in DNA-strand cleavage. No stimulation by rifamycin SV occurred in the absence of microsomes, NADH or ferric-ATP. Stimulation occurred with other ferric complexes besides ferric-ATP, e.g. ferric-histidine, ferric-citrate, ferric-EDTA, and ferric-(NH4)2SO4. Rifamycin SV did not significantly increase the high rates of DNA strand cleavage found with NADPH as the microsomal reductant. The stimulation of NADH-dependent microsomal DNA strand cleavage was completely blocked by catalase, superoxide dismutase, GSH and a variety of hydroxyl-radical-scavenging agents, but not by anti-oxidants that prevent microsomal lipid peroxidation. Redox cycling agents, such as menadione and paraquat, in contrast with rifamycin SV, stimulated the NADPH-dependent reaction; menadione and rifamycin SV were superior to paraquat in stimulating the NADH-dependent reaction. These results indicate that rifamycin SV can, in the presence of an iron catalyst, increase microsomal production of reactive oxygen species which can cause DNA-strand cleavage. In contrast with other redox cycling agents, the stimulation by rifamycin SV is more pronounced with NADH than with NADPH as the

  11. pSv3neo transfection and radiosensitivity of human cancer cell lines

    International Nuclear Information System (INIS)

    Parris, C.N.; Masters, J.R.W.; Green, M.H.L.

    1990-01-01

    Immortalisation of human fibroblasts by transfection with a plasmid, pSV3neo, results in an increase in their radioresistance. The change in radiosensitivity may either be a consequence of transformation or due to expression of the SV40 T-antigen in pSV3neo. To investigate these two possibilities, we transfected pSV3neo into cells already transformed and immortalised. The radiosensitivies of three human bladder cancer cell lines were unaltered in clones expressing T-antigen, indicating that the changes observed in fibroblasts probably are a consequence of transformation, and not the presence of SV40 T-antigen. (author)

  12. Proposta de linguagem geradora de imagens em impressoras de página

    OpenAIRE

    Mario Antonio Stefani

    1990-01-01

    Uma compacta linguagem descritora de páginas, destinada a impressoras não-impacto de estrutura raster é apresentada. Tal linguagem foi implementada usando o processador gráfico TMS4010, da Texas Instruments e possui uma estrutura muito similar à encontrada nas linguagens interpretativas encadeadas. A linguagem é totalmente modular e interativa, e se utiliza um modelo gráfico simples, visando simular as tarefas normalmente encontradas nas artes tipográficas. São efetuadas comparações com outra...

  13. Specifications for Testing Procedures at Svåheia

    DEFF Research Database (Denmark)

    Margheritini, Lucia; Kofoed, Jens Peter

    This report is realized in order to define testing procedures of the SSG pilot in Svåheia location. This will be done by listing all the relevant parameters related to the structure performance.......This report is realized in order to define testing procedures of the SSG pilot in Svåheia location. This will be done by listing all the relevant parameters related to the structure performance....

  14. Título da página: Gay, Lesbian and Straight Education Network

    Directory of Open Access Journals (Sweden)

    Ana Cristina Santos

    2012-10-01

    Full Text Available Embora centrada nos Estados Unidos da América, esta página disponibiliza importantes recursos pedagógicos – para docentes e estudantes – sobre o combate à discriminação em meio escolar. Estão acessíveis artigos referentes a políticas sociais, grupos locais, estudos académicos, relatórios nacionais e iniciativas de estudantes. Há ainda uma biblioteca virtual organizada por temas, na qual se pode aceder a artigos e manuais para formação de formadores.

  15. A novel splice variant of supervillin, SV5, promotes carcinoma cell proliferation and cell migration

    International Nuclear Information System (INIS)

    Chen, Xueran; Yang, Haoran; Zhang, Shangrong; Wang, Zhen; Ye, Fang; Liang, Chaozhao; Wang, Hongzhi; Fang, Zhiyou

    2017-01-01

    Supervillin is an actin-associated protein that regulates actin dynamics by interacting with Myosin II, F-actin, and Cortactin to promote cell contractility and cell motility. Two splicing variants of human Supervillin (SV1 and SV4) have been reported in non-muscle cells; SV1 lacks 3 exons present in the larger isoform SV4. SV2, also called archvillin, is present in striated muscle; SV3, also called smooth muscle archvillin or SmAV, was cloned from smooth muscle. In the present study, we identify a novel splicing variant of Supervillin (SV5). SV5 contains a new splicing pattern. In the mouse tissues and cell lines examined, SV5 was predominantly expressed in skeletal and cardiac muscles and in proliferating cells, but was virtually undetectable in most normal tissues. Using RNAi and rescue experiments, we show here that SV5 displays altered functional properties in cancer cells, and regulates cell proliferation and cell migration.

  16. Stimulation of NADH-dependent microsomal DNA strand cleavage by rifamycin SV.

    OpenAIRE

    Kukiełka, E; Cederbaum, A I

    1995-01-01

    Rifamycin SV is an antibiotic anti-bacterial agent used in the treatment of tuberculosis. This drug can autoxidize, especially in the presence of metals, and generate reactive oxygen species. A previous study indicated that rifamycin SV can increase NADH-dependent microsomal production of reactive oxygen species. The current study evaluated the ability of rifamycin SV to interact with iron and increase microsomal production of hydroxyl radical, as detected by conversion of supercoiled plasmid...

  17. Development of direct reading dosimeters for the dose 0-3 mSv and 0-5 mSv ranges for personnel monitoring

    International Nuclear Information System (INIS)

    Gaikwad, P.V.; Shirkar, Y.B.; Patil, A.S.; Madgaonkar, P.P.; Kale, K.L.; Guhagarkar, H.V.; Gandhi, D.P.; Gupta, S.K.; Kothiyal, G.P.; Sahni, V.C.

    1998-01-01

    Direct reading dosimeters (DRDs) are widely used to measure cumulative dose received by personnel working at nuclear reactor sites or in other environment having x- and gamma rays. A DRD operates on the principle of gold leaf electroscope, and is a small, rugged, hermetically sealed, self reading type device easily carried by an individual in his pocket. The development of dosimeters suitable for the dose ranges 0-3 mSv and 0-5 mSv is reported

  18. Estudio del discurso escrito en páginas de internet como apoyo para la enseñanza de temas científicos: evolución y origen de la vida

    OpenAIRE

    Valeiras, Nora; Micieli, Carolina; Skicko, Marcela

    2011-01-01

    El presente trabajo analiza el discurso escrito que proponen un grupo de páginas Web sobre “Origen de la vida y Evolución”. Se definen categorías que permiten analizar el uso del enguaje, la procedencia y el diseño de las páginas. El método de estudio que se propone es descriptivo utilizándose técnicas cualitativas, fijándose características de las páginas Web, de tal forma que se puedan seleccionar como materiales para ser usadas en la generación de un programa de formación permanente de doc...

  19. Título da página electrónica: Development Gateway

    Directory of Open Access Journals (Sweden)

    João Paulo Dias

    2003-10-01

    Full Text Available Esta página é desenvolvida pela Development Gateway Foundation, que se intitula uma organização sem fins lucrativos e tem como missão a redução da pobreza e o apoio ao desenvolvimento sustentável baseado na disseminação de informação e de tecnologias da comunicação. A lista de entidades que participam neste projecto é muito diversificada e apresenta-se em níveis muito distintos. Desde instituições académicas e de investigação (Biblioteca Alexandrina, Pontifícia Universidade Católica do Peru, ...

  20. Test of models for replication of SV40 DNA following UV irradiation

    International Nuclear Information System (INIS)

    Barnett, S.W.

    1983-01-01

    The replication of SV40 DNA immediately after irradiation of infected monkey cells has been examined. SV40 DNA synthesis is inhibited in a UV fluence-dependent fashion, and the synthesis of completely replicated (Form I) SV40 molecules is more severely inhibited than is total SV40 DNA synthesis. Two models for DNA replication-inhibition have been tested. Experimental results have been compared to those predicted by mathematical models derived to describe two possible molecular mechanisms of replication inhibition. No effect of UV irradiation on the uptake and phosphorylation of 3 H-thymidine nor on the size of the intracellular deoxythymidine triphosphate pool of SV40-infected cells have been observed, validating the use of 3 H-thymidine incorporation as a measure of DNA synthesis in this system. In vitro studies have been performed to further investigate the mechanism of dimer-specific inhibition of completion of SV40 DNA synthesis observed in in vivo. The results of these studies are consistent with a mechanism of discontinuous synthesis past dimer sites, but it is equally possible that the mechanism of DNA replication of UV-damaged DNA in the in vitro system is different from that which occurs in vivo

  1. Modelo de análisis de las páginas web turísticas (diputaciones y ayuntamientos de las capitales de provincia de Andalucía

    Directory of Open Access Journals (Sweden)

    Almudena Soto Benito

    2010-11-01

    Full Text Available El análisis de las diecisiete páginas web de Andalucía  nos proporcionará las claves, no sólo sobre el tipo de turismo que desde los organismos oficiales se pretende vender, sino también los aspectos técnicos-formales que aparecen en dichas páginas. Los apartados que vamos a analizar en las diecisiete páginas Web están entre los primeros lugares del ranking de los principales buscadores (Google, Yahoo, bing, Ask, etc., son fíceles de navegar, rápidos a la hora de cargarse, visualmente atractivos, interactivos, detallistas y orientados a un público concreto.Palabras Claves: Andalucía, páginas web, internet, accesibilidad, empresas turísticas.___________________________ABSTRACT:The analysis of the seventeen websites in Andalusia will give us the keys, not only on the type of tourism that the agencies intend to sell, but also the technical-formal aspects shown in these pages. The sections we will analyze among the seventeen websites are in the first places in the ranking of major searching engines (Google, Yahoo, MSN, Ask, etc... Those are user friendly, quick to load, attractive, interactive, detailed and thought for a specific target.Keywords: Andalusia, websites, internet, accessibility, tourism enterprises

  2. Título da página electrónica: ProVentium Consortium

    Directory of Open Access Journals (Sweden)

    Susana Freiria

    2012-12-01

    Full Text Available O Consórcio ProVentium, criado em 2000, resulta de uma iniciativa que reúne governos, organizações do sector privado, da sociedade civil e de âmbito académico. O facto de reunir instituições provenientes de sectores tão diversos contribui para criar uma plataforma de diálogo que incorpora diferentes pontos de vista. Este consórcio tem como objectivo aumentar a segurança das comunidades mais vulneráveis e reduzir os impactos dos desastres. A página principal encontra-se dividida em três secçõ...

  3. SV2: accurate structural variation genotyping and de novo mutation detection from whole genomes.

    Science.gov (United States)

    Antaki, Danny; Brandler, William M; Sebat, Jonathan

    2018-05-15

    Structural variation (SV) detection from short-read whole genome sequencing is error prone, presenting significant challenges for population or family-based studies of disease. Here, we describe SV2, a machine-learning algorithm for genotyping deletions and duplications from paired-end sequencing data. SV2 can rapidly integrate variant calls from multiple structural variant discovery algorithms into a unified call set with high genotyping accuracy and capability to detect de novo mutations. SV2 is freely available on GitHub (https://github.com/dantaki/SV2). jsebat@ucsd.edu. Supplementary data are available at Bioinformatics online.

  4. Marketing digital e marketing de relacionamento: interação e engajamento como determinantes do crescimento de páginas do Facebook

    Directory of Open Access Journals (Sweden)

    Felipe Ribeiro Soares

    2015-07-01

    Full Text Available As transformações deflagradas pela internet, permitindo a aproximação de clientes e fornecedores, têm gerado crescentes reflexões acadêmicas e empresariais que culminam na proposição do uso de mídias sociais como ferramentas de marketing. Nesse sentido, este estudo buscou verificar o impacto das ações realizadas pela organização nas redes sociais relativamente às interações e ao engajamento de usuários, e seus reflexos para o crescimento da popularidade da marca no ambiente virtual. Tomando como unidade de análise a página da Fiat (Brasil no Facebook, perfazendo um corte longitudinal por 53 semanas, pôde-se concluir que as interações do administrador da página da empresa e o engajamento dos usuários promovem o crescimento de popularidade e engajamento da marca no ambiente virtual. Dessa maneira, as organizações precisam entender como suas ações nas redes sociais estão ecoando, apresentando indícios de quais tipos de conteúdos, horários e dias da semana da publicação determinaram maior crescimento da página do Facebook analisada.

  5. Solid state fermentation and production of rifamycin SV using Amycolatopsis mediterranei.

    Science.gov (United States)

    Nagavalli, M; Ponamgi, S P D; Girijashankar, V; Venkateswar Rao, L

    2015-01-01

    Production of Rifamycin SV from cheaper agro-industrial by-products using mutant strain of Amycolatopsis mediterranei OVA5-E7 in solid state fermentation (SSF) was optimized. Among the agro-based substrates used, ragi bran was found suitable for maximizing the yield of Rifamycin SV (1310 mg 100 g(-1) ds). The yield can be further enhanced to 19·7 g Kg(-1) of dry substrate by supplementing the substrate with deoiled cotton cake (10% w/w) using optimized fermentation parameters such as maintaining 80% moisture, pH 7·0, 30°C incubation temperature, inoculum 25% v/w and carrying the solid state fermenting for 9 days. Manipulating these seven specifications, the end product yield achieved in our experimentation was 20 g of Rifamycin SV Kg(-1) ds. Eventually, an overall 5-fold improvement in Rifamycin SV production was achieved. Antibiotics such as rifamycin are broad-spectrum antimicrobial drugs used in large-scale worldwide as human medicine towards controlling diseases. Amycolatopsis mediterranei strain which produces this antibiotic was earlier used in submerged fermentation yielded lower amounts of rifamycin. By employing cheaper agro-industrial by-products, we produced upto 20 g rifamycin SV per Kg dry substrate used under optimized solid state fermentation conditions. Keeping in view, the role of rifamycin in meeting the medical demands of world's increasing population; we successfully used an improved strain on cheaper substrates with optimized fermentation parameters and achieved a 5-fold improvement in rifamycin SV production. © 2014 The Society for Applied Microbiology.

  6. The local and the global: Gina Nahai and the taking up of serpents and stereotypes

    OpenAIRE

    Yousaf, N

    2007-01-01

    Region, home and transnational migration are explored in terms of the transcultural complexities that reverberate through Iranian American Gina Nahai's Sunday's Silence. Nahai grapples with stereotypes that attach to the Holiness churches in the east Tennessee region of Appalachia. This essay argues that the novel's politics rest on the intersubjectivity of strangers as bound into a metaphysics of desire. It is through this paradigm that Nahai writes against the reductive association of “mino...

  7. Genetic influences on ovulation of primary oocytes in LT/Sv strain mice.

    Science.gov (United States)

    Everett, Clare A; Auchincloss, Catherine A; Kaufman, Matthew H; Abbott, Catherine M; West, John D

    2004-11-01

    A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

  8. Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.

    Science.gov (United States)

    Cole, C N; Tornow, J; Clark, R; Tjian, R

    1986-01-01

    The biochemical properties of the large T antigens encoded by simian virus 40 (SV40) mutants with deletions at DdeI sites in the SV40 A gene were determined. Mutant large T antigens containing only the first 138 to 140 amino acids were unable to bind to the SV40 origin of DNA replication as were large T antigens containing at their COOH termini 96 or 97 amino acids encoded by the long open reading frame located between 0.22 and 0.165 map units (m.u.). All other mutant large T antigens were able to bind to the SV40 origin of replication. Mutants with in-phase deletions at 0.288 and 0.243 m.u. lacked ATPase activity, but ATPase activity was normal in mutants lacking origin-binding activity. The 627-amino acid large T antigen encoded by dlA2465, with a deletion at 0.219 m.u., was the smallest large T antigen displaying ATPase activity. Mutant large T antigens with the alternate 96- or 97-amino acid COOH terminus also lacked ATPase activity. All mutant large T antigens were found in the nuclei of infected cells; a small amount of large T with the alternate COOH terminus was also located in the cytoplasm. Mutant dlA2465 belonged to the same class of mutants as dlA2459. It was unable to form plaques on CV-1p cells at 37 or 32 degrees C but could form plaques on BSC-1 monolayers at 37 degrees C but not at 32 degrees C. It was positive for viral DNA replication and showed intracistronic complementation with any group A mutant whose large T antigen contained a normal carboxyl terminus. These findings and those of others suggest that both DNA binding and ATPase activity are required for the viral DNA replication function of large T antigen, that these two activities must be located on the same T antigen monomer, and that these two activities are performed by distinct domains of the polypeptide. These domains are distinct and separable from the domain affected by the mutation of dlA2465 and indicate that SV40 large T antigen is made up of at least three separate functional

  9. Documentación Informativa. Práctica 3: Evaluación de páginas web: parámetros de calidad

    OpenAIRE

    Alemany, Dolores

    2010-01-01

    Observación y análisis de indicadores fundamentales para reconocer la calidad de un sitio web: AUTORÍA, ACCESIBILIDAD, CONTENIDO, ACTUALIZACIÓN, DISEÑO, ERGONOMÍA, USABILIDAD y VISIBILIDAD, en tres páginas web.

  10. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC.

    Science.gov (United States)

    Arthur, Kelly K; Kendrick, Brent S; Gabrielson, John P

    2015-01-01

    The levels and types of aggregates present in protein biopharmaceuticals must be assessed during all stages of product development, manufacturing, and storage of the finished product. Routine monitoring of aggregate levels in biopharmaceuticals is typically achieved by size exclusion chromatography (SEC) due to its high precision, speed, robustness, and simplicity to operate. However, SEC is error prone and requires careful method development to ensure accuracy of reported aggregate levels. Sedimentation velocity analytical ultracentrifugation (SV-AUC) is an orthogonal technique that can be used to measure protein aggregation without many of the potential inaccuracies of SEC. In this chapter, we discuss applications of SV-AUC during biopharmaceutical development and how characteristics of the technique make it better suited for some applications than others. We then discuss the elements of a comprehensive analytical control strategy for SV-AUC. Successful implementation of these analytical control elements ensures that SV-AUC provides continued value over the long time frames necessary to bring biopharmaceuticals to market. © 2015 Elsevier Inc. All rights reserved.

  11. High throughput testing of the SV40 Large T antigen binding to cellular p53 identifies putative drugs for the treatment of SV40-related cancers

    International Nuclear Information System (INIS)

    Carbone, Michele; Rudzinski, Jennifer; Bocchetta, Maurizio

    2003-01-01

    SV40 has been linked to some human malignancies, and the evidence that this virus plays a causative role in mesothelioma and brain tumors is mounting. The major SV40 oncoprotein is the Large tumor antigen (Tag). A key Tag transforming activity is connected to its capability to bind and inactivate cellular p53. In this study we developed an effective, high throughput, ELISA-based method to study Tag-p53 interaction in vitro. This assay allowed us to screen a chemical library and to identify a chemical inhibitor of the Tag binding to p53. We propose that our in vitro assay is a useful method to identify molecules that may be used as therapeutic agents for the treatment of SV40-related human cancers

  12. Análisis y medición de calidad y accesibilidad de páginas web públicas con énfasis en orientación inclusiva

    OpenAIRE

    Ingavélez Guerra, Paola Cristina

    2013-01-01

    El desarrollo de páginas web en nuestro país se ha constituido en una forma de publicidad moderna, que responde a la búsqueda de mayores mercados y clientes potenciales. La constante velocidad de la tecnología y su interacción, no permite espacios de reflexión sobre la igualdad de oportunidades para todas las personas en este aspecto, pues cada uno busca terminar un producto y que sea atractivo para el común denominador. Es así como nos vemos sumergidos en muchas páginas web agradables a la v...

  13. Genome-Wide Comparative Functional Analyses Reveal Adaptations of Salmonella sv. Newport to a Plant Colonization Lifestyle

    Directory of Open Access Journals (Sweden)

    Marcos H. de Moraes

    2018-05-01

    Full Text Available Outbreaks of salmonellosis linked to the consumption of vegetables have been disproportionately associated with strains of serovar Newport. We tested the hypothesis that strains of sv. Newport have evolved unique adaptations to persistence in plants that are not shared by strains of other Salmonella serovars. We used a genome-wide mutant screen to compare growth in tomato fruit of a sv. Newport strain from an outbreak traced to tomatoes, and a sv. Typhimurium strain from animals. Most genes in the sv. Newport strain that were selected during persistence in tomatoes were shared with, and similarly selected in, the sv. Typhimurium strain. Many of their functions are linked to central metabolism, including amino acid biosynthetic pathways, iron acquisition, and maintenance of cell structure. One exception was a greater need for the core genes involved in purine metabolism in sv. Typhimurium than in sv. Newport. We discovered a gene, papA, that was unique to sv. Newport and contributed to the strain’s fitness in tomatoes. The papA gene was present in about 25% of sv. Newport Group III genomes and generally absent from other Salmonella genomes. Homologs of papA were detected in the genomes of Pantoea, Dickeya, and Pectobacterium, members of the Enterobacteriacea family that can colonize both plants and animals.

  14. Comparing Global Initiative for Asthma (GINA) criteria with the Childhood Asthma Control Test (C-ACT) and Asthma Control Test (ACT)

    NARCIS (Netherlands)

    Koolen, B.B.; Pijnenburg, M.W.; Brackel, H.J.; Landstra, A.M.; Berg, N.J. van den; Merkus, P.J.F.M.; Hop, W.C.J.; Vaessen-Verberne, A.A.

    2011-01-01

    Several tools are useful in detecting uncontrolled asthma in children. The aim of this study was to compare Global Initiative for Asthma (GINA) guidelines with the Childhood Asthma Control Test (C-ACT) and the Asthma Control Test (ACT) in detecting uncontrolled asthma in children. 145 children with

  15. Una discutible ocupación de las páginas de cultura en el diario madrileño El País

    Directory of Open Access Journals (Sweden)

    Dr. José Manuel de Pablos Coello - jpablos@ull.es

    2000-01-01

    Full Text Available El autor, despué de hacer una introducción al concepto de cultura y al trato que en prensa suele darse a la divulgación cultural, muestra cómo en el diario madrileño El País hay una cierta confusión entre los contenidos de las páginas de cultura y de espectáculos, que van seguidas y en ocasiones muestran textos intercambiados. Analiza cómen la sección "La cultura" se inserta con frecuencia y en el tiempo del tercer director (Jesús Ceberio informaciones sobre moda femenina, siempre ilustradas con bellas modelos que muestran partes de su cuerpo al desnudo. Esa estrategia informativa la califica el autor como una premeditada idea de erotizar las páginas del periódico.

  16. Synaptic vesicle glycoprotein 2C (SV2C) modulates dopamine release and is disrupted in Parkinson disease.

    Science.gov (United States)

    Dunn, Amy R; Stout, Kristen A; Ozawa, Minagi; Lohr, Kelly M; Hoffman, Carlie A; Bernstein, Alison I; Li, Yingjie; Wang, Minzheng; Sgobio, Carmelo; Sastry, Namratha; Cai, Huaibin; Caudle, W Michael; Miller, Gary W

    2017-03-14

    Members of the synaptic vesicle glycoprotein 2 (SV2) family of proteins are involved in synaptic function throughout the brain. The ubiquitously expressed SV2A has been widely implicated in epilepsy, although SV2C with its restricted basal ganglia distribution is poorly characterized. SV2C is emerging as a potentially relevant protein in Parkinson disease (PD), because it is a genetic modifier of sensitivity to l-DOPA and of nicotine neuroprotection in PD. Here we identify SV2C as a mediator of dopamine homeostasis and report that disrupted expression of SV2C within the basal ganglia is a pathological feature of PD. Genetic deletion of SV2C leads to reduced dopamine release in the dorsal striatum as measured by fast-scan cyclic voltammetry, reduced striatal dopamine content, disrupted α-synuclein expression, deficits in motor function, and alterations in neurochemical effects of nicotine. Furthermore, SV2C expression is dramatically altered in postmortem brain tissue from PD cases but not in Alzheimer disease, progressive supranuclear palsy, or multiple system atrophy. This disruption was paralleled in mice overexpressing mutated α-synuclein. These data establish SV2C as a mediator of dopamine neuron function and suggest that SV2C disruption is a unique feature of PD that likely contributes to dopaminergic dysfunction.

  17. Visualization of SV2A conformations in situ by the use of Protein Tomography

    International Nuclear Information System (INIS)

    Lynch, Berkley A.; Matagne, Alain; Braennstroem, Annika; Euler, Anne von; Jansson, Magnus; Hauzenberger, Elenor; Soederhaell, J. Arvid

    2008-01-01

    The synaptic vesicle protein 2A (SV2A), the brain-binding site of the anti-epileptic drug levetiracetam (LEV), has been characterized by Protein Tomography TM . We identified two major conformations of SV2A in mouse brain tissue: first, a compact, funnel-structure with a pore-like opening towards the cytoplasm; second, a more open, V-shaped structure with a cleft-like opening towards the intravesicular space. The large differences between these conformations suggest a high degree of flexibility and support a valve-like mechanism consistent with the postulated transporter role of SV2A. These two conformations are represented both in samples treated with LEV, and in saline-treated samples, which indicates that LEV binding does not cause a large-scale conformational change of SV2A, or lock a specific conformational state of the protein. This study provides the first direct structural data on SV2A, and supports a transporter function suggested by sequence homology to MFS class of transporter proteins

  18. Stratification of eosinophilic asthma patients treated with reslizumab and GINA Step 4 or 5 therapy.

    Science.gov (United States)

    Brusselle, Guy; Canvin, Janice; Weiss, Sivan; Sun, Shawn X; Buhl, Roland

    2017-07-01

    Reslizumab, an anti-interleukin-5 monoclonal antibody, significantly reduces exacerbation frequency and improves lung function, asthma control and quality of life in adults with severe eosinophilic asthma, as demonstrated in Phase III studies. This secondary analysis assessed reslizumab's efficacy in patients receiving baseline treatment per Global Initiative for Asthma (GINA) Step 4 and Step 5 guidelines. Pooled data from duplicate, Phase III, reslizumab versus placebo studies in patients with severe eosinophilic asthma (blood eosinophils ≥400 cells·µL -1 ) were stratified by baseline therapy. Efficacy assessments were exacerbation rates and changes from baseline forced expiratory volume in 1 s (FEV 1 ) and patient-reported outcomes. Of 953 patients, 69% (n=657) and 11% (n=106) were receiving Step 4 and Step 5 therapy, respectively. Compared with placebo, reslizumab reduced exacerbation rates by 53% (95% CI 0.36-0.62) and 72% (95% CI 0.15-0.52), in Step 4 and Step 5 groups respectively. By study end, reslizumab increased FEV 1 in Step 4 and Step 5 groups by 103 mL (95% CI 52-154 mL) and 237 mL (95% CI 68-407 mL), respectively. Reslizumab also improved patient-reported outcomes compared with placebo in both groups. Reslizumab reduces exacerbation rates and improves lung function and patient-reported outcomes in patients with eosinophilic asthma receiving therapy per Steps 4 and 5 of the GINA guidelines.

  19. O cinema da literatura: quando a página se torna tela

    Directory of Open Access Journals (Sweden)

    André Soares Vieira

    2016-06-01

    Full Text Available Este ensaio busca problematizar uma forma de relação entre o cinema e a literatura, no momento em que essa se vale de procedimentos próprios à linguagem e ao imaginário daquele. Ao considerarmos obras literárias como as que foram produzidas por escritores como Marguerite Duras, Hubert Aquin e Marcio Souza, percebemos zonas de contato que vão desde a referência a um roteiro fictício até o ato de contar filmes. Nesta prespectiva, as obras examinadas evocam outras facetas da « máquina cinematográfica » em suas relações com a literatura, apresentando-se como filmes de textos projetados nas páginas do livro.

  20. Aanvullend commentaar op art. 511a Sv

    NARCIS (Netherlands)

    van Laanen, F.; Melai, A.L.; Groenhuijsen, M.S.

    2005-01-01

    Schr. geeft een actueel wetenschappelijk commentaar op art. 511a Sv. Daarin komt met name de hedendaagse werkingssfeer aan de orde van deze bepaling, waarin is gesteld dat de berechting van strafbare feiten die ingevolge enige wet aan de burgerlijke rechter is opgedragen, ter terechtzitting van de

  1. Tipos, organização e visibilidade de informações em páginas iniciais de websites de universidades brasileiras / Types, organization, and visibility of information of brasilian universities website

    Directory of Open Access Journals (Sweden)

    Lígia Dias de Freitas

    2013-05-01

    Full Text Available Apresenta pesquisa realizada com o objetivo de investigar a relação entre o tipo de informação disponibilizada nas páginas iniciais de websites de universidades brasileiras e a forma como ela está organizada, especificamente: verificar a visibilidade dada a cada tipo de informação por meio da área utilizada nas páginas iniciais dos websites das universidades escolhidas; identificar a visão das universidades em relação a seus websites; e relacionar tipos de informação disponibilizadas e tipos de organização nas páginas iniciais dos websites das universidades. Para isto, o artigo discorre sobre Arquitetura de Informação, a presença das universidades na web e o papel de suas páginas iniciais. A motivação da pesquisa baseia-se na identificação de um padrão para as informações organizadas nos websites de universidades, visto que estes agregam conteúdo para diferentes públicos-alvo. As universidades escolhidas como objeto de pesquisa foram as dez primeiras brasileiras no Webometrics Ranking of World Universities, de julho de 2010. A metodologia consiste em analisar as capturas de telas das páginas iniciais e as respostas a três questões abertas feitas aos setores de comunicação das universidades. Os resultados mostram que o tipo de informação mais freqüente é o Administrativo e institucional e a organização predominante é por Assunto. Acredita-se que o método de análise aqui proposto é útil para o estudo de websites e pode ser aplicado a diferentes tipos de instituições.

  2. The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems.

    Directory of Open Access Journals (Sweden)

    Tamer Z Salem

    Full Text Available The simian virus 40 polyadenylation signal (SV40 polyA has been routinely inserted downstream of the polyhedrin promoter in many baculovirus expression vector systems (BEVS. In the baculovirus prototype Autographa californica multiple nucleopolyhedrovirus (AcMNPV, the polyhedrin promoter (very late promoter transcribes its gene by a viral RNA polymerase therefore there is no supporting evidence that SV40 polyA is required for the proper gene expression under the polyhedrin promoter. Moreover, the effect of the SV40 polyA sequence on the polyhedrin promoter activity has not been tested either at its natural polyhedrin locus or in other loci in the viral genome. In order to test the significance of adding the SV40 polyA sequence on gene expression, the expression of the enhanced green fluorescent protein (egfp was evaluated with and without the presence of SV40 polyA under the control of the polyhedrin promoter at different genomic loci (polyherin, ecdysteroid UDP-glucosyltransferase (egt, and gp37. In this study, spectrofluorometry and western blot showed reduction of EGFP protein for all recombinant viruses with SV40 polyA, whereas qPCR showed an increase in the egfp mRNA levels. Therefore, we conclude that SV40 polyA increases mRNA levels but decreases protein production in the BEVS when the polyhedrin promoter is used at different loci. This work suggests that SV40 polyA in BEVSs should be replaced by an AcMNPV late gene polyA for optimal protein production or left untouched for optimal RNA production (RNA interference applications.

  3. The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems

    Science.gov (United States)

    Salem, Tamer Z.; Seaborn, Craig P.; Turney, Colin M.; Xue, Jianli; Shang, Hui; Cheng, Xiao-Wen

    2015-01-01

    The simian virus 40 polyadenylation signal (SV40 polyA) has been routinely inserted downstream of the polyhedrin promoter in many baculovirus expression vector systems (BEVS). In the baculovirus prototype Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the polyhedrin promoter (very late promoter) transcribes its gene by a viral RNA polymerase therefore there is no supporting evidence that SV40 polyA is required for the proper gene expression under the polyhedrin promoter. Moreover, the effect of the SV40 polyA sequence on the polyhedrin promoter activity has not been tested either at its natural polyhedrin locus or in other loci in the viral genome. In order to test the significance of adding the SV40 polyA sequence on gene expression, the expression of the enhanced green fluorescent protein (egfp) was evaluated with and without the presence of SV40 polyA under the control of the polyhedrin promoter at different genomic loci (polyherin, ecdysteroid UDP-glucosyltransferase (egt), and gp37). In this study, spectrofluorometry and western blot showed reduction of EGFP protein for all recombinant viruses with SV40 polyA, whereas qPCR showed an increase in the egfp mRNA levels. Therefore, we conclude that SV40 polyA increases mRNA levels but decreases protein production in the BEVS when the polyhedrin promoter is used at different loci. This work suggests that SV40 polyA in BEVSs should be replaced by an AcMNPV late gene polyA for optimal protein production or left untouched for optimal RNA production (RNA interference applications). PMID:26659470

  4. Análisis, diseño e implementación de una página web y su gestión para un grupo de centros médicos

    OpenAIRE

    Salinero Comesaña, Javier

    2012-01-01

    Aquest projecte tracta l'actualització d'una pàgina Web d'un centre mèdic, que amb el pas del temps és obsoleta, i que vol obrir nous centres. Este proyecto trata la actualización de una página Web de un centro médico, que con el paso del tiempo está obsoleta, y que quiere abrir nuevos centros. This project aims at updating a medical center website, which over time is no longer up-to-date, and tries to open new centers.

  5. Acceso a páginas pornográficas en Internet y Comunicación familiar sobre sexualidad en adolescentes del distrito de "El Agustino", Lima - Perú 2006-2007

    OpenAIRE

    Santa Maria Alvarez, Jorge Arturo; Laguna Urdanivia, Keylla Jessica; Escalante Romero, Lorena del Rocio; Zimic Zare, Carolina Teresa; Luna Rengifo, Denisse; Echazu Irala, Carlos; Salazar Granara, Alberto

    2008-01-01

    Se ha realizado un estudio descriptivo transversal, en 10 colegios del Distrito de El Agustino-Lima; desarrollándose un cuestionario en una población total de 1107 adolescentes, siendo el 50% (n=553) de sexo masculino y 50% (n=554) de sexo femenino, indagando sobre el acceso a las páginas pornográficas en Internet, el lugar donde lo realizan y la comunicación familiar global entre progenitores y los mismos. El 30.4% (n=337) de los adolescentes visitan páginas pornográficas; siendo los lugares...

  6. Título da página electrónica: Escola de Cultura de Paz

    Directory of Open Access Journals (Sweden)

    Mónica Rafael Simões

    2012-10-01

    Full Text Available A Escola de Cultura de Paz foi criada em 1999, dando seguimento à Cátedra UNESCO sobre Paz e Direitos Humanos da Universidade Autónoma de Barcelona, com o objectivo de trabalhar para uma cultura de paz, promovendo a investigação e formação em áreas relacionadas com prevenção e análise de conflitos, processos de paz, desarmamento, direitos humanos e educação para a paz. Esta página é coordenada por Vicenç Fisas e produzida pela sua equipa de jovens investigadores da Universidade Autónoma de B...

  7. Escala de confiabilidad de libros de texto y páginas web desde la transposición didáctica de modelos moleculares de Kossel, Lewis y Pauling

    Directory of Open Access Journals (Sweden)

    Myriam Moreno Amado

    2016-03-01

    Full Text Available Resumen El presente trabajo tuvo como propósito elaborar una escala de confiabilidad de libros de texto y páginas web desde el análisis de la transposición didáctica de algunos modelos moleculares presentados por W. Kossel, G. N. Lewis y L. C. Pauling. Se tomaron como referencia documentos publicados en sus primeras versiones, los cuales fueron analizados desde una perspectiva histórico-epistemológica, para identificar aspectos medulares de su formulación. La escala de confiabilidad se estableció con seis criterios enmarcados en cuatro categorías de análisis, donde se incluyen los aspectos identificados para cada modelo molecular. Se elaboraron dos escalas de confiabilidad, la escala de mención de aspectos (EMA y la escala de ponderación (EP utilizadas para el análisis de 15 libros de texto y 40 páginas web. Según las escalas de confiabilidad se encontró que, los modelos presentados en páginas web y libros de texto revisados presentan, respectivamente, máximo un 10,85% y 5,87% de satisfacción.

  8. Tipos, organização e visibilidade de informações em páginas iniciais de websites de universidades brasileiras / Types, organization, and visibility of information of brasilian universities website

    OpenAIRE

    Lígia Dias de Freitas

    2013-01-01

    Apresenta pesquisa realizada com o objetivo de investigar a relação entre o tipo de informação disponibilizada nas páginas iniciais de websites de universidades brasileiras e a forma como ela está organizada, especificamente: verificar a visibilidade dada a cada tipo de informação por meio da área utilizada nas páginas iniciais dos websites das universidades escolhidas; identificar a visão das universidades em relação a seus websites; e relacionar tipos de informação disponibilizadas e tipos ...

  9. SV40-transformed human fibroblasts: evidence for cellular aging in pre-crisis cells.

    Science.gov (United States)

    Stein, G H

    1985-10-01

    Pre-crisis SV40-transformed human diploid fibroblast (HDF) cultures have a finite proliferative lifespan, but they do not enter a viable senescent state at end of lifespan. Little is known about either the mechanism for this finite lifespan in SV40-transformed HDF or its relationship to finite lifespan in normal HDF. Recently we proposed that in normal HDF the phenomena of finite lifespan and arrest in a viable senescent state depend on two separate processes: 1) an age-related decrease in the ability of the cells to recognize or respond to serum and/or other mitogens such that the cells become functionally mitogen-deprived at the end of lifespan; and 2) the ability of the cells to enter a viable, G1-arrested state whenever they experience mitogen deprivation. In this paper, data are presented that suggest that pre-crisis SV40-transformed HDF retain the first process described above, but lack the second process. It is shown that SV40-transformed HDF have a progressively decreasing ability to respond to serum as they age, but they continue to traverse the cell cycle at the end of lifespan. Concomitantly, the rate of cell death increases steadily toward the end of lifespan, thereby causing the total population to cease growing and ultimately to decline. Previous studies have shown that when SV40-transformed HDF are environmentally serum deprived, they likewise exhibit continued cell cycle traverse coupled with increased cell death. Thus, these results support the hypothesis that pre-crisis SV40-transformed HDF still undergo the same aging process as do normal HDF, but they end their lifespan in crisis rather than in the normal G1-arrested senescent state because they have lost their ability to enter a viable, G1-arrested state in response to mitogen deprivation.

  10. Antička baština samostana sv. Frane u Splitu

    OpenAIRE

    Cambi, Nenad

    2005-01-01

    Na mjestu današnjega franjevačkog samostana sv. Frane u Splitu otkriveno je nekoliko starokršćanskih spomenika: dva natpisa (danas izgubljena), sarkofag s prikazom motiva Prelaska Izraelaca preko Crvenoga mora, fragment s prikazom Krista i apostola te najvjerojatnije i motivom Davida i Golijata, pilastar s urezanim križem, fragment akroterija sarkofaga s križem te omanji fragment pluteja s ljiljanom. Današnja crkva sv. Frane uništila je prethodni samostan i crkvu, koje je u XI. st. podigao sp...

  11. Methods of calculation of cross section of reaction 115In(gamma, n)114mIn

    International Nuclear Information System (INIS)

    Zhaba, V.I.; Parlag, A.M.

    2015-01-01

    The cross section of reaction 115 In(gamma, n) 114m In is expected by different methods. Results of the got cross section it is well comported inter se the Penfold-Leiss and Tikhonov's methods. The calculation of cross section is conducted the Penfold-Leiss method with smoothing out by the method of iterations. Number of iterations n = 1; 3; 5. In the programmatic package of TALYS-1.4 got cross section for five models of closeness of levels. Theoretical and experimental results well coincide in a maximum.

  12. Validity and reliability of the Brazilian version of Yale-Brown obsessive compulsive scale-shopping version (YBOCS-SV).

    Science.gov (United States)

    Leite, Priscilla Lourenço; Filomensky, Tatiana Zambrano; Black, Donald W; Silva, Adriana Cardoso

    2014-08-01

    The Yale-Brown Obsessive Compulsive Scale-Shopping Version (YBOCS-SV) is considered the gold standard in the assessment of shopping severity. It is designed to assess cognitions and behaviors relating to compulsive buying behavior. The present study aims to assess the validity of the Brazilian version of this scale. For the study, composed the sample 610 participants: 588 subjects of a general population and 22 compulsive buyers. Factorial analysis was performed to assess the relations and the correlation between the YBOCS-SV, the Compulsive Buying Scale (CBS), and Richmond Compulsive Buying Scale (RCBS), was assessed using Pearson coefficient, for study of convergent and divergent validity. Cronbach's alpha coefficients were used to assess internal consistency. The results show good to excellent psychometric parameters for the YBOCS-SV in its Brazilian version. With regard to correlations, the YBOCS-SV is inversely and proportionally correlated with CBS and the RCBS, indicating that the YBOCS-SV is an excellent instrument for screening compulsive buying. The YBOCS-SV presented high alpha coefficient of Cronbach's alpha (0.92), demonstrating good reliability. The Brazilian version of the YBOCS-SV is indicated to diagnose compulsive buying disorder, and likely use for the purposes intended in the Brazilian population. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Analysis of plasma membrane Ca(2+)-ATPase expression in control and SV40-transformed human fibroblasts.

    Science.gov (United States)

    Reisner, P D; Brandt, P C; Vanaman, T C

    1997-01-01

    It has been long known that neoplastic transformation is accompanied by a lowered requirement for extracellular Ca2+ for growth. The studies presented here demonstrate that human fibroblastic cell lines produce the two commonly found 'housekeeping' isoforms of the plasma membrane Ca(2+)-ATPase (PMCA), PMCA1b and 4b, and at the expression of both is demonstrably lower in cell lines neoplastically transformed by SV40 than in the corresponding parental cell lines. Western blot analyses of lysates from control (GM00037) and SV40-transformed (GM00637) skin fibroblasts revealed a 138 kDa PMCA whose level was significantly lower in the SV40-transformed cells relative to either total cellular protein or alpha-tubulin. Similar analyses of plasma membrane preparations from control WI-38) and SV40-transformed (WI-38VA13) lung fibroblasts revealed 3-4-fold lower levels of PMCA in the SV40-transformed cells. Competitive ELISAs performed on detergent solubilized plasma membrane preparations indicated at least 3-4-fold lower levels of PMCA in the SV40-transformed cell lines compared to controls. Reverse transcriptase coupled-PCR analyses showed that PMCA1b and PMCA4b were the only isoforms expressed in all four cell lines. The PMCA4b mRNA level detected by Northern analysis also was substantially lower in SV40 transformed skin fibroblasts than in non-transformed fibroblasts. Quantitative RT-PCR analyses showed levels of PMCA1b and 4b mRNAs to be 5 and 10-fold lower, respectively, in GM00637 than in GM00037 when the levels of PCR products were normalized to glyceraldehyde-3-phosphate dehydrogenase (G3PDH) mRNA. These results demonstrate that the expression of these distinct PMCA genes is substantially lower in SV40 transformed human skin and lung fibroblasts and may be coordinately regulated in these cells.

  14. Dynamic VaR Measurement of Gold Market with SV-T-MN Model

    Directory of Open Access Journals (Sweden)

    Fenglan Li

    2017-01-01

    Full Text Available VaR (Value at Risk in the gold market was measured and predicted by combining stochastic volatility (SV model with extreme value theory. Firstly, for the fat tail and volatility persistence characteristics in gold market return series, the gold price return volatility was modeled by SV-T-MN (SV-T with Mixture-of-Normal distribution model based on state space. Secondly, future sample volatility prediction was realized by using approximate filtering algorithm. Finally, extreme value theory based on generalized Pareto distribution was applied to measure dynamic risk value (VaR of gold market return. Through the proposed model on the price of gold, empirical analysis was investigated; the results show that presented combined model can measure and predict Value at Risk of the gold market reasonably and effectively and enable investors to further understand the extreme risk of gold market and take coping strategies actively.

  15. Evolutionary models of early-type contact binary SV Centauri

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, Y; Saio, H [Tohoku Univ., Sendai (Japan). Faculty of Science; Sugimoto, Daiichiro

    1978-12-01

    Models of the early-type contact binary system SV Centauri are computed with a binary-star evolution program. The effects of mass exchange, i.e., the effects of mass acceptance as well as mass loss, are properly included. With the initial masses of the component stars as 12.4 and 8.0 M sub(solar mass), the following observed configurations are well reproduced; the component stars are definitely in contact and the rate of mass exchange is 4 x 10/sup -4/ M sub(solar mass)yr/sup -1/. The more massive component is less luminous and has a lower effective temperature. Such features are also reproduced quantitatively. Agreement of the computed models with observation indicates that the binary system SV Cen is actually in the phase of rapid mass exchange preceding the mass-ratio reversal.

  16. Characterization of SV-40 Tag rats as a model to study prostate cancer

    International Nuclear Information System (INIS)

    Harper, Curt E; Patel, Brijesh B; Cook, Leah M; Wang, Jun; Shirai, Tomoyuki; Eltoum, Isam A; Lamartiniere, Coral A

    2009-01-01

    Prostate cancer is the second most frequently diagnosed cancer in men. Animal models that closely mimic clinical disease in humans are invaluable tools in the fight against prostate cancer. Recently, a Simian Virus-40 T-antigen (SV-40 Tag) targeted probasin promoter rat model was developed. This model, however, has not been extensively characterized; hence we have investigated the ontogeny of prostate cancer and determined the role of sex steroid receptor and insulin-like growth factor-1 (IGF-1) signaling proteins in the novel SV-40 Tag rat. The SV-40 Tag rat was histopathologically characterized for time to tumor development, incidence and multiplicity and in the ventral, dorsal, lateral and anterior lobes of the prostate. Immunoassay techniques were employed to measure cell proliferation, apoptosis, and sex steroid receptor and growth factor signaling-related proteins. Steroid hormone concentrations were measured via coated well enzyme linked immunosorbent assay (ELISA) kits. Prostatic intraepithelial neoplasia (PIN) and well-differentiated prostate cancer developed as early as 2 and 10 weeks of age, respectively in the ventral prostate (VP) followed by in the dorsolateral (DLP). At 8 weeks of age, testosterone and dihydrotestosterone (DHT) concentrations in SV-40 Tag rats were increased when compared to non-transgenic rats. High cell proliferation and apoptotic indices were found in VP and DLP of transgenic rats. Furthermore, we observed increased protein expression of androgen receptor, IGF-1, IGF-1 receptor, and extracellular signal-regulated kinases in the prostates of SV-40 Tag rats. The rapid development of PIN and prostate cancer in conjunction with the large prostate size makes the SV-40 Tag rat a useful model for studying prostate cancer. This study provides evidence of the role of sex steroid and growth factor proteins in prostate cancer development and defines appropriate windows of opportunity for preclinical trials and aids in the rational design of

  17. Clarín y Página/12 en el debate sobre la Ley de Servicios de Comunicación Audiovisual

    Directory of Open Access Journals (Sweden)

    Brenda Inés Di Paolo

    2017-07-01

    Full Text Available [es] El trabajo de investigación pretende dar cuenta de las modalidades enunciativas adoptadas por dos de los principales diarios de alcance nacional –Clarín y Página/12– en uno de los momentos de mayor tensión en la relación entre los medios concentrados de comunicación y el gobierno de Cristina Fernández de Kirchner, el debate sobre la Ley de Servicios de Comunicación Audiovisual (LSCA. La búsqueda se orienta a establecer criterios que delimiten la especificidad conceptual del campo de lo político y su relación con el campo de la comunicación. Pretende indagar en la tensión entre poder corporativo y poder de democratización del espacio radioeléctrico a través de un análisis de la construcción de títulos y noticias en los diarios Clarín y Página/12 en el debate de audiencias públicas de la LSCA en la Cámara de Diputados, el día 8 de septiembre de 2009. El estudio recorre un corpus de 14 noticias, en las cuales observaremos la producción de los encuadres (volantas, títulos y bajadas y su correspondencia con los cuerpos o textos. El abordaje teórico apunta a problematizar la relación: medios y política articulando las categorías de Eliseo Verón para el análisis de la prensa y su propuesta conceptual sobre la enunciación política. [en] Our research addresses the discursive modes adopted by two of the main national newspapers—Clarín and Pá- gina/12—on the occasion of one of the most stressing moments of the relation between concentrated mass media and Cristina Fernández de Kirchner’s administration: the debate over the Law of Audiovisual Communication Services (LSCA, in Spanish. Our research seeks to establish criteria for the conceptual specification of the political field as related to the communication field. It tackles the tension between corporative and democratic power over the radio space by analyzing the headlines and news in Clarín and Página/12 during the LSCA public hearings in The House

  18. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants

    International Nuclear Information System (INIS)

    Kawano, Masaaki; Morikawa, Katsuma; Suda, Tatsuya; Ohno, Naohito; Matsushita, Sho; Akatsuka, Toshitaka; Handa, Hiroshi; Matsui, Masanori

    2014-01-01

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A ⁎ 02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A ⁎ 02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimeric SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. - Highlights: • We constructed chimeric SV40-VLPs carrying an influenza virus-derived CTL epitope. • Chimeric SV40-VLPs induce influenza-specific CTLs in mice without adjuvants. • Chimeric SV40-VLPs induce heterosubtypic protection against influenza A viruses. • Chimeric SV40-VLPs induce long-lasting memory CTLs. • Chimeric SV40-VLPs is a promising vaccine platform with self-adjuvant properties

  19. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants

    Energy Technology Data Exchange (ETDEWEB)

    Kawano, Masaaki [Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan); Morikawa, Katsuma [Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501 (Japan); Suda, Tatsuya [Department of Microbiology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan); Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Ohno, Naohito [Laboratory for Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Matsushita, Sho [Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan); Allergy Center, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan); Akatsuka, Toshitaka [Department of Microbiology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan); Handa, Hiroshi, E-mail: handa.h.aa@m.titech.ac.jp [Solutions Research Laboratory, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8503 (Japan); Matsui, Masanori, E-mail: mmatsui@saitama-med.ac.jp [Department of Microbiology, Faculty of Medicine, Saitama Medical University, Moroyama-cho, Iruma-gun, Saitama 350-0495 (Japan)

    2014-01-05

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A{sup ⁎}02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A{sup ⁎}02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimeric SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. - Highlights: • We constructed chimeric SV40-VLPs carrying an influenza virus-derived CTL epitope. • Chimeric SV40-VLPs induce influenza-specific CTLs in mice without adjuvants. • Chimeric SV40-VLPs induce heterosubtypic protection against influenza A viruses. • Chimeric SV40-VLPs induce long-lasting memory CTLs. • Chimeric SV40-VLPs is a promising vaccine platform with self-adjuvant properties.

  20. A mouse model for chronic lymphocytic leukemia based on expression of the SV40 large T antigen

    DEFF Research Database (Denmark)

    ter Brugge, Petra J; Ta, Van B T; de Bruijn, Marjolein J W

    2009-01-01

    The simian virus 40 (SV40) T antigen is a potent oncogene able to transform many cell types and has been implicated in leukemia and lymphoma. In this report, we have achieved sporadic SV40 T-antigen expression in mature B cells in mice, by insertion of a SV40 T antigen gene in opposite...... transcriptional orientation in the immunoglobulin (Ig) heavy (H) chain locus between the D and J(H) segments. SV40 T-antigen expression appeared to result from retention of the targeted germline allele and concomitant antisense transcription of SV40 large T in mature B cells, leading to chronic lymphocytic...... leukemia (CLL). Although B-cell development was unperturbed in young mice, aging mice showed accumulation of a monoclonal B-cell population in which the targeted IgH allele was in germline configuration and the wild-type IgH allele had a productive V(D)J recombination. These leukemic B cells were Ig...

  1. A monoclonal antibody against SV40 large T antigen (PAb416) does not label Merkel cell carcinoma.

    Science.gov (United States)

    Pelletier, Daniel J; Czeczok, Thomas W; Bellizzi, Andrew M

    2018-07-01

    Merkel cell carcinoma represents poorly differentiated neuroendocrine carcinoma of cutaneous origin. In most studies, the vast majority of Merkel cell carcinomas are Merkel cell polyomavirus (MCPyV)-associated. SV40 polyomavirus immunohistochemistry is typically used in the diagnosis of other polyomavirus-associated diseases, including tubulointerstitial nephritis and progressive multifocal leukoencephalopathy, given cross-reactivity with BK and JC polyomaviruses. MCPyV-specific immunohistochemistry is commercially available, but, if antibodies against SV40 also cross-reacted with MCPyV, that would be advantageous from a resource-utilisation perspective. Tissue microarrays were constructed from 39 Merkel cell carcinomas, 24 small-cell lung carcinomas, and 18 extrapulmonary visceral small-cell carcinomas. SV40 large T antigen immunohistochemistry (clone PAb416) was performed; MCPyV large T antigen immunohistochemistry (clone CM2B4) had been previously performed. UniProt was used to compare the amino acid sequences of the SV40, BK, JC and MCPyV large T antigens, focusing on areas recognised by the PAb416 and CM2B4 clones. SV40 immunohistochemistry was negative in all tumours; MCPyV immunohistochemistry was positive in 38% of Merkel cell carcinomas and in 0% of non-cutaneous poorly differentiated neuroendocrine carcinomas. UniProt analysis revealed a high degree of similarity between SV40, BK, and JC viruses in the region recognised by PAb416. There was less homology between SV40 and MCPyV in this region, which was also interrupted by two long stretches of amino acids unique to MCPyV. The CM2B4 clone recognises a unique epitope in one of these stretches. The PAb416 antibody against the SV40 large T antigen does not cross-react with MCPyV large T antigen, and thus does not label Merkel cell carcinoma. © 2018 John Wiley & Sons Ltd.

  2. The monetary value of the man-mSv for Korean NPP radiation workers assessed by the radiation aversion factor

    International Nuclear Information System (INIS)

    Lee, B. I.; Suh, D. H.; Kim, S. I.; Jeong, M. S.; Lim, Y. K.

    2008-01-01

    The monetary value of the man-mSv for operators of Korean nuclear power plants (NPPs) was calculated using a radiation aversion factor based on a survey of NPP workers. Initially, the life expectancy in the population is 79.4 y, the average age of cancer occurrence is 60 y, the average annual wage for an electric worker is 56 000 $ y -1 and the nominal risk coefficient induced by radiation is 4.2 E-5 mSv were used to evaluate the basic monetary value (α base) resulting in 45.6 $ mSv -1 . To investigate the degree of radiation aversion, the subject of the investigation was selected as the working radiation workers in 10 NPPs in Korea (Kori 1-2, Yeonggwang 1-3, Ulchin 1-3 and Wolseong 1-2). In August 2010, with the cooperation of KHNP and partner companies, a total of 2500 survey questionnaires to 10 NPPs (or 250 surveys to each NPP) were distributed to currently employed radiation workers. From these, 2157 responses were obtained between August and October 2010. The assessed radiation aversion factor and the monetary value of the man-mSv from the calculated radiation aversion factor were 1.26 and ∼50 $ in the 0-1 mSv range, 1.38 and ∼200 $ in the 1-5 mSv range, 1.52 and ∼1000 $ in the 5-10 mSv range, 1.65 and ∼4000 $ in the 10-20 mSv range and 1.74 and ∼8500 $ >20 mSv. (authors)

  3. Título da página electrónica: Centro de Documentação Eremias Delizoicov – Comissão de Familiares dos Mortos e Desaparecidos Políticos

    Directory of Open Access Journals (Sweden)

    António Sousa Ribeiro

    2012-10-01

    Full Text Available Trata‑se de uma página cujo objectivo é divulgar as investigações realizadas sobre as mortes e a localização dos restos mortais das vítimas e identificar os responsáveis pelos crimes de tortura, homicídio e ocultação dos cadáveres de dezenas de pessoas durante o período da ditadura militar no Brasil (1964/85. A página tem na sua base de dados os nomes de 383 mortos e desaparecidos, textos sobre a amnistia, a guerrilha do Araguaia, a vala clandestina do Cemitério de Perus, a história das orga...

  4. Measuring marine iron(III) complexes by CLE-AdSV

    NARCIS (Netherlands)

    Town, R.M.; Leeuwen, van H.P.

    2005-01-01

    Iron(iii) speciation data, as determined by competitive ligand exchange?adsorptive stripping voltammetry (CLE-AdSV), is reconsidered in the light of the kinetic features of the measurement. The very large stability constants reported for iron(iii) in marine ecosystems are shown to be possibly due to

  5. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated ras oncogene and SV40 T-antigen.

    Science.gov (United States)

    Su, L N; Little, J B

    1992-08-01

    Three normal human diploid cell strains were transfected with an activated Ha-ras oncogene (EJ ras) or SV40 T-antigen. Multiple clones were examined for morphological alterations, growth requirements, ability to grow under anchorage independent conditions, immortality and tumorigenicity in nude mice. Clones expressing SV40 T-antigen alone or in combination with ras protein p21 were significantly radioresistant as compared with their parent cells or clones transfected with the neo gene only. This radioresistant phenotype persisted in post-crisis, immortalized cell lines. Cells transfected with EJ ras alone showed no morphological alterations nor significant changes in radiosensitivity. Cell clones expressing ras and/or SV40 T-antigen showed a reduced requirement for serum supplements, an increase in aneuploidy and chromosomal aberrations, and enhanced growth in soft agar as an early cellular response to SV40 T-antigen expression. The sequential order of transfection with SV40 T-antigen and ras influenced radio-sensitivity but not the induction of morphological changes. These data suggest that expression of the SV40 T-antigen but not activated Ha-ras plays an important role in the radiosensitivity of human diploid cells. The radioresistant phenotype in SV40 T transfected cells was not related to the enhanced level of genetic instability seen in pre-crisis and newly immortalized cells, nor to the process of immortalization itself.

  6. Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies

    Directory of Open Access Journals (Sweden)

    Miguel G. Toscano

    2017-09-01

    Full Text Available Replication-defective (RD recombinant simian virus 40 (SV40-based gene delivery vectors hold a great potential for clinical applications because of their presumed non-immunogenicity and capacity to induce immune tolerance to the transgene products in humans. However, the clinical use of SV40 vectors has been hampered by the lack of a packaging cell line that produces replication-competent (RC free SV40 particles in the vector production process. To solve this problem, we have adapted the current SV40 vector genome used for the production of vector particles and generated a novel Vero-based packaging cell line named SuperVero that exclusively expresses the SV40 large T antigen. SuperVero cells produce similar numbers of SV40 vector particles compared to the currently used packaging cell lines, albeit in the absence of contaminating RC SV40 particles. Our unique SV40 vector platform named SVac paves the way to clinically test a whole new generation of SV40-based therapeutics for a broad range of important diseases.

  7. GABA(A)-benzodiazepine receptor complex sensitivity in 5-HT(1A) receptor knockout mice on a 129/Sv background.

    NARCIS (Netherlands)

    Pattij, T.; Groenink, L.; Oosting, R.S.; Gugten, J. van der; Maes, R.A.A.; Olivier, B.

    2002-01-01

    Previous studies in 5-HT(1A) receptor knockout (1AKO) mice on a mixed Swiss Websterx129/Sv (SWx129/Sv) and a pure 129/Sv genetic background suggest a differential gamma-aminobutyric acid (GABA(A))-benzodiazepine receptor complex sensitivity in both strains, independent from the anxious phenotype. To

  8. Host-cell reactivation of ultraviolet-irradiated SV 40 DNA in five complementation groups of xeroderma pigmentosum

    International Nuclear Information System (INIS)

    Abrahams, P.J.; Eb, A.J. van der

    1976-01-01

    Host-cell reactivation of UV-irradiated double-stranded SV40 DNA was studied in BSC-1 monkey cells, normal human cells, heterozygous Xeroderma pigmentosum xp cells, representative cell strains of the five complemention groups of XP and in XP 'variant' cells. The following percentages of survival of the plaque-forming ability of double-stranded SV40 DNA were found in XP cells compared with the value found in normal monkey and human cells: groupA, 13%; group B, 30%; group C, 18%; group D, 14%; group E, 59%; and in the heterozygous XP cells almost 100%. The survival in XP 'variant' cells was 66%. The survival of single-stranded SV40 DNA in BSC-1 cells was much lower than that of double-stranded SV40 DNA in XP cells of complementation group A, which possibly indicates that some repair of UV damage occurs even in XP cells of group A

  9. When public health and genetic privacy collide: positive and normative theories explaining how ACA's expansion of corporate wellness programs conflicts with GINA's privacy rules.

    Science.gov (United States)

    Bard, Jennifer S

    2011-01-01

    The Patient Protection and Affordable Care Act of 2010 (ACA) contains many provisions intended to increase access to and lower the cost of health care by adopting public health measures. One of these promotes the use of at-work wellness programs by both providing employers with grants to develop these programs and also increasing their ability to tie the price employees pay for health insurance for participating in these programs and meeting specific health goals. Yet despite ACA's specific alteration of three different statues which had in the past shielded employees from having to contribute to the cost of their health insurance based on their achieving employer-designated health markers, it chose to leave alone recently enacted rules implementing the Genetic Non-Discrimination Act (GINA), which prohibits employers from asking employees about their family health history in any context, including assessing their risk for setting wellness targets. This article reviews how both the changes made by ACA and the restrictions recently put place by GINA will affect the way employers are likely to structure Wellness Programs. It also considers how these changes reflect the competing social goals of both ACA, which seeks to expand access to the population by lowering costs, and GINA, which seeks to protect individuals from discrimination. It does so by analyzing both positive theories about how these new laws will function and normative theories explaining the likelihood of future friction between the interests of the population of the United States as a whole who are in need of increased and affordable access to health care, and of the individuals living in this country who risk discrimination, as science and medicine continue to make advances in linking genetic make-up to risk of future illness. © 2011 American Society of Law, Medicine & Ethics, Inc.

  10. SV40 host-substituted variants: a new look at the monkey DNA inserts and recombinant junctions.

    Science.gov (United States)

    Singer, Maxine; Winocour, Ernest

    2011-04-10

    The available monkey genomic data banks were examined in order to determine the chromosomal locations of the host DNA inserts in 8 host-substituted SV40 variant DNAs. Five of the 8 variants contained more than one linked monkey DNA insert per tandem repeat unit and in all cases but one, the 19 monkey DNA inserts in the 8 variants mapped to different locations in the monkey genome. The 50 parental DNAs (32 monkey and 18 SV40 DNA segments) which spanned the crossover and flanking regions that participated in monkey/monkey and monkey/SV40 recombinations were characterized by substantial levels of microhomology of up to 8 nucleotides in length; the parental DNAs also exhibited direct and inverted repeats at or adjacent to the crossover sequences. We discuss how the host-substituted SV40 variants arose and the nature of the recombination mechanisms involved. Copyright © 2011 Elsevier Inc. All rights reserved.

  11. Evaluación de la calidad de información sobre el embarazo en páginas web según las guías peruanas Evaluation of the quality of information about pregnancy found in webpages according to the Peruvian guidelines

    Directory of Open Access Journals (Sweden)

    Rodrigo M. Carrillo-Larco

    2012-03-01

    Full Text Available Objetivos. El objetivo del presente trabajo es evaluar la calidad de páginas web en español, que contengan información sobre los síntomas del embarazo. Materiales y métodos. Se realizó un estudio transversal y analítico, empleando la metodología previamente validada por Curró et al. para evaluar la calidad de páginas web; estas páginas fueron recuperadas a través de búsquedas en Google, MSN.com y Yahoo.com. La evaluación de la calidad incluyó tres componentes: contenido médico, responsabilidad y usabilidad de la página web. El contenido médico fue contrastado con la información disponible en las guías nacionales de Atención Integral de la Salud Sexual y Reproductiva, del Ministerio de Salud del Perú. Resultados. Se evaluaron 99 páginas web con contenido en español. El 26,3% presentó media adherencia al patrón de oro. En el 32,3% se encontraron los tres factores evaluados de las guías nacionales; 37,4% eran páginas web de mala calidad. El 24,2% eran blogs los cuales estaban asociados, como factor protector, a páginas web de mala calidad (OR: 0,1, IC 95%: 0,02-0,5. Conclusiones. La mayoría de páginas web evaluadas en español, relacionadas con síntomas del embarazo, son de mala calidad. Estos resultados indican que se requiere educar adecuadamente a los pacientes y al público en general así como evaluar la calidad de información que se encuentra en Internet.Objectives. The objective of this study is to evaluate the information quality about pregnancy symptoms in Spanish available in the internet. Materials and methods. This is an analytic, cross-sectional study. In order to evaluate the selected websites, we used a methodology previously validated by Curró et al. The websites were selected from Google, MSN.com and Yahoo.com. The quality evaluation included three components: medical contents, accountability and usability of the website. The medical content was compared with the data available in the national

  12. A high-quality narrow passband filter for elastic SV waves via aligned parallel separated thin polymethylmethacrylate plates

    Directory of Open Access Journals (Sweden)

    Jun Zhang

    2017-08-01

    Full Text Available We designed a high-quality filter that consists of aligned parallel polymethylmethacrylate (PMMA thin plates with small gaps for elastic SV waves propagate in metals. Both the theoretical model and the full numerical simulation show the transmission spectrum of the elastic SV waves through such a filter has several sharp peaks with flawless transmission within the investigated frequencies. These peaks can be readily tuned by manipulating the geometry parameters of the PMMA plates. Our investigation finds that the same filter performs well for different metals where the elastic SV waves propagated.

  13. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants.

    Science.gov (United States)

    Kawano, Masaaki; Morikawa, Katsuma; Suda, Tatsuya; Ohno, Naohito; Matsushita, Sho; Akatsuka, Toshitaka; Handa, Hiroshi; Matsui, Masanori

    2014-01-05

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A*02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A*02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimeric SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. © 2013 Elsevier Inc. All rights reserved.

  14. Impaired CK1 delta activity attenuates SV40-induced cellular transformation in vitro and mouse mammary carcinogenesis in vivo.

    Directory of Open Access Journals (Sweden)

    Heidrun Hirner

    Full Text Available Simian virus 40 (SV40 is a powerful tool to study cellular transformation in vitro, as well as tumor development and progression in vivo. Various cellular kinases, among them members of the CK1 family, play an important role in modulating the transforming activity of SV40, including the transforming activity of T-Ag, the major transforming protein of SV40, itself. Here we characterized the effects of mutant CK1δ variants with impaired kinase activity on SV40-induced cell transformation in vitro, and on SV40-induced mammary carcinogenesis in vivo in a transgenic/bi-transgenic mouse model. CK1δ mutants exhibited a reduced kinase activity compared to wtCK1δ in in vitro kinase assays. Molecular modeling studies suggested that mutation N172D, located within the substrate binding region, is mainly responsible for impaired mutCK1δ activity. When stably over-expressed in maximal transformed SV-52 cells, CK1δ mutants induced reversion to a minimal transformed phenotype by dominant-negative interference with endogenous wtCK1δ. To characterize the effects of CK1δ on SV40-induced mammary carcinogenesis, we generated transgenic mice expressing mutant CK1δ under the control of the whey acidic protein (WAP gene promoter, and crossed them with SV40 transgenic WAP-T-antigen (WAP-T mice. Both WAP-T mice as well as WAP-mutCK1δ/WAP-T bi-transgenic mice developed breast cancer. However, tumor incidence was lower and life span was significantly longer in WAP-mutCK1δ/WAP-T bi-transgenic animals. The reduced CK1δ activity did not affect early lesion formation during tumorigenesis, suggesting that impaired CK1δ activity reduces the probability for outgrowth of in situ carcinomas to invasive carcinomas. The different tumorigenic potential of SV40 in WAP-T and WAP-mutCK1δ/WAP-T tumors was also reflected by a significantly different expression of various genes known to be involved in tumor progression, specifically of those involved in wnt-signaling and DNA

  15. SV40 Assembly In Vivo and In Vitro

    Directory of Open Access Journals (Sweden)

    Ariella Oppenheim

    2008-01-01

    Full Text Available The Simian virus 40 (SV40 capsid is a T = 7d icosahedral lattice ∼45 nm in diameter surrounding the ∼5 kb circular minichromosome. The outer shell is composed of 360 monomers of the major capsid protein VP1, tightly bound in 72 pentamers. VP1 is a jellyroll β-barrel, with extending N- and C-terminal arms. The N-terminal arms bind DNA and face the interior of the capsid. The flexible C-arms tie together the 72 pentamers in three distinct kinds of interactions, thus facilitating the formation of a T = 7 icosahedron from identical pentameric building blocks. Assembly in vivo was shown to occur by addition of capsomers around the DNA. We apply a combination of biochemical and genetic approaches to study SV40 assembly. Our in vivo and in vitro studies suggest the following model: one or two capsomers bind at a high affinity to ses, the viral DNA encapsidation signal, forming the nucleation centre for assembly. Next, multiple capsomers attach concomitantly, at lower affinity, around the minichromosome. This increases their local concentration facilitating rapid, cooperative assembly reaction. Formation of the icosahedron proceeds either by gradual addition of single pentamers to the growing shell or by concerted assembly of pentamer clusters.

  16. Transformation of SV40-immortalized human uroepithelial cells by 3-methylcholanthrene increases IFN- and Large T Antigen-induced transcripts

    Directory of Open Access Journals (Sweden)

    Easton Marilyn J

    2010-02-01

    Full Text Available Abstract Background Simian Virus 40 (SV40 immortalization followed by treatment of cells with 3-methylcholanthrene (3-MC has been used to elicit tumors in athymic mice. 3-MC carcinogenesis has been thoroughly studied, however gene-level interactions between 3-MC and SV40 that could have produced the observed tumors have not been explored. The commercially-available human uroepithelial cell lines were either SV40-immortalized (HUC or SV40-immortalized and then 3-MC-transformed (HUC-TC. Results To characterize the SV40 - 3MC interaction, we compared human gene expression in these cell lines using a human cancer array and confirmed selected changes by RT-PCR. Many viral Large T Antigen (Tag expression-related changes occurred in HUC-TC, and it is concluded that SV40 and 3-MC may act synergistically to transform cells. Changes noted in IFP 9-27, 2'-5' OAS, IF 56, MxA and MxAB were typical of those that occur in response to viral exposure and are part of the innate immune response. Because interferon is crucial to innate immune host defenses and many gene changes were interferon-related, we explored cellular growth responses to exogenous IFN-γ and found that treatment impeded growth in tumor, but not immortalized HUC on days 4 - 7. Cellular metabolism however, was inhibited in both cell types. We conclude that IFN-γ metabolic responses were functional in both cell lines, but IFN-γ anti-proliferative responses functioned only in tumor cells. Conclusions Synergism of SV40 with 3-MC or other environmental carcinogens may be of concern as SV40 is now endemic in 2-5.9% of the U.S. population. In addition, SV40-immortalization is a generally-accepted method used in many research materials, but the possibility of off-target effects in studies carried out using these cells has not been considered. We hope that our work will stimulate further study of this important phenomenon.

  17. N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A

    Science.gov (United States)

    Yao, Guorui; Zhang, Sicai; Mahrhold, Stefan; Lam, Kwok-ho; Stern, Daniel; Bagramyan, Karine; Perry, Kay; Kalkum, Markus; Rummel, Andreas; Dong, Min; Jin, Rongsheng

    2016-01-01

    Botulinum neurotoxin serotype A1 (BoNT/A1) is one of the most dangerous potential bioterrorism agents, and exerts its action by invading motoneurons. It is also a licensed drug widely used for medical and cosmetic applications. Here we report a 2.0 Å resolution crystal structure of BoNT/A1 receptor-binding domain in complex with its neuronal receptor, the glycosylated human SV2C. We find that the neuronal tropism of BoNT/A1 requires recognition of both the peptide moiety and an N-linked glycan on SV2. This N-glycan—conserved in all SV2 isoforms across vertebrates—is essential for BoNT/A1 binding to neurons and its potent neurotoxicity. The glycan-binding interface on SV2 is targeted by a human BoNT/A1-neutralizing antibody currently licensed as an anti-botulism drug. Our studies reveal a new paradigm of host-pathogen interactions, in which pathogens exploit conserved host post-translational modifications to achieve highly specific receptor binding while also tolerating genetic changes across multiple isoforms of receptors. PMID:27294781

  18. COLONIZAÇÃO OU DIÁSPORAS? RESENHA C.-G. SCHWENTZEL (org., M. DANA, St.LEBRETON, Fr.PRÊTEUX, Les diásporas grecques VIII-III s. Paris, Atlande, 2012, 446 páginas, 25 euros. (Dossiê: O Mundo Antigo: Literatura e Historiografia

    Directory of Open Access Journals (Sweden)

    José Antonio Dabdab Trabulsi

    2015-09-01

    Full Text Available C.-G. SCHWENTZEL (org., M. DANA, St.LEBRETON, Fr.PRÊTEUX, Les diásporas grecques VIII-III s. Paris, Atlande, 2012, 446 páginas, 25 euros.C.-G. SCHWENTZEL (org., M. DANA, St.LEBRETON, Fr.PRÊTEUX, Les diásporas grecques VIII-III s. Paris, Atlande, 2012, 446 páginas, 25 euros. Recebido em: 02/04/2015  – Aceito em 13/05/2015

  19. PSHA in Israel by using the synthetic ground motions from simulated seismicity: the modified SvE procedure

    Science.gov (United States)

    Meirova, T.; Shapira, A.; Eppelbaum, L.

    2018-05-01

    In this study, we updated and modified the SvE approach of Shapira and van Eck (Nat Hazards 8:201-215, 1993) which may be applied as an alternative to the conventional probabilistic seismic hazard assessment (PSHA) in Israel and other regions of low and moderate seismicity where measurements of strong ground motions are scarce. The new computational code SvE overcomes difficulties associated with the description of the earthquake source model and regional ground-motion scaling. In the modified SvE procedure, generating suites of regional ground motion is based on the extended two-dimensional source model of Motazedian and Atkinson (Bull Seism Soc Amer 95:995-1010, 2005a) and updated regional ground-motion scaling (Meirova and Hofstteter, Bull Earth Eng 15:3417-3436, 2017). The analytical approach of Mavroeidis and Papageorgiou (Bull Seism Soc Amer 93:1099-1131, 2003) is used to simulate the near-fault acceleration with the near-fault effects. The comparison of hazard estimates obtained by using the conventional method implemented in the National Building Code for Design provisions for earthquake resistance of structures and the modified SvE procedure for rock-site conditions indicates a general agreement with some perceptible differences at the periods of 0.2 and 0.5 s. For the periods above 0.5 s, the SvE estimates are systematically greater and can increase by a factor of 1.6. For the soft-soil sites, the SvE hazard estimates at the period of 0.2 s are greater than those based on the CB2008 ground-motion prediction equation (GMPE) by a factor of 1.3-1.6. We suggest that the hazard estimates for the sites with soft-soil conditions calculated by the modified SvE procedure are more reliable than those which can be found by means of the conventional PSHA. This result agrees with the opinion that the use of a standard GMPE applying the NEHRP soil classification based on the V s, 30 parameter may be inappropriate for PSHA at many sites in Israel.

  20. Early superoxide dismutase alterations during SV40-transformation of human fibroblasts.

    Science.gov (United States)

    Bravard, A; Hoffschir, F; Sabatier, L; Ricoul, M; Pinton, A; Cassingena, R; Estrade, S; Luccioni, C; Dutrillaux, B

    1992-11-11

    The expression of superoxide dismutases (SOD) 1 and 2 was studied in 4 clones of human fibroblasts after their infection by simian virus 40 (SV40), in parallel with the alterations of chromosomes 21 and chromosome 6q arms, carrying the genes that encode for SOD1 and SOD2 respectively. For all clones, a similar scheme with 2 main phases was observed for both chromosome and SOD variations. The first phase, defined as the pre-crisis phase, was characterized by chromosomal instability, but maintenance of normal numbers of chromosome 6q arms and chromosomes 21. The level of SOD2 mRNA was high, while SOD2 activity and immunoreactive protein were low. SOD1 protein and activity were decreased. In the second phase, defined as the post-crisis phase, the accumulation of clonal chromosomal rearrangements led to the loss of 6q arms, while the number of chromosomes 21 remained normal. SOD2 mRNA level was decreased and SOD2 immunoreactive protein and activity remained low. SOD1 protein and activity increased with passages, reaching values similar to those of control cells at late passages. As in established SV40-transformed human fibroblast cell lines, good correlation was found between SOD2 activity and the relative number of 6q arms. These results allow us to reconstruct the sequence of events leading to the decrease of SOD2, a possible tumor-suppressor gene, during the process of SV40-transformation of human fibroblasts.

  1. Measurement Invariance of the Short Version of the Problematic Mobile Phone Use Questionnaire (PMPUQ-SV) across Eight Languages.

    Science.gov (United States)

    Lopez-Fernandez, Olatz; Kuss, Daria J; Pontes, Halley M; Griffiths, Mark D; Dawes, Christopher; Justice, Lucy V; Männikkö, Niko; Kääriäinen, Maria; Rumpf, Hans-Jürgen; Bischof, Anja; Gässler, Ann-Kathrin; Romo, Lucia; Kern, Laurence; Morvan, Yannick; Rousseau, Amélie; Graziani, Pierluigi; Demetrovics, Zsolt; Király, Orsolya; Schimmenti, Adriano; Passanisi, Alessia; Lelonek-Kuleta, Bernadeta; Chwaszcz, Joanna; Chóliz, Mariano; Zacarés, Juan José; Serra, Emilia; Dufour, Magali; Rochat, Lucien; Zullino, Daniele; Achab, Sophia; Landrø, Nils Inge; Suryani, Eva; Hormes, Julia M; Terashima, Javier Ponce; Billieux, Joël

    2018-06-08

    The prevalence of mobile phone use across the world has increased greatly over the past two decades. Problematic Mobile Phone Use (PMPU) has been studied in relation to public health and comprises various behaviours, including dangerous, prohibited, and dependent use. These types of problematic mobile phone behaviours are typically assessed with the short version of the Problematic Mobile Phone Use Questionnaire (PMPUQ⁻SV). However, to date, no study has ever examined the degree to which the PMPU scale assesses the same construct across different languages. The aims of the present study were to (i) determine an optimal factor structure for the PMPUQ⁻SV among university populations using eight versions of the scale (i.e., French, German, Hungarian, English, Finnish, Italian, Polish, and Spanish); and (ii) simultaneously examine the measurement invariance (MI) of the PMPUQ⁻SV across all languages. The whole study sample comprised 3038 participants. Descriptive statistics, correlations, and Cronbach's alpha coefficients were extracted from the demographic and PMPUQ-SV items. Individual and multigroup confirmatory factor analyses alongside MI analyses were conducted. Results showed a similar pattern of PMPU across the translated scales. A three-factor model of the PMPUQ-SV fitted the data well and presented with good psychometric properties. Six languages were validated independently, and five were compared via measurement invariance for future cross-cultural comparisons. The present paper contributes to the assessment of problematic mobile phone use because it is the first study to provide a cross-cultural psychometric analysis of the PMPUQ-SV.

  2. Qualidade da informação da internet disponível para pacientes em páginas em português Quality of internet information available to patients on websites in Portuguese

    Directory of Open Access Journals (Sweden)

    Adriana Del Giglio

    2012-12-01

    Full Text Available OBJETIVO: Pacientes e seus familiares frequentemente procuram na internet informações a respeito de suas doenças. Diabetes mellitus (DM, hipertensão arterial sistêmica (HAS e infarto agudo do miocárdio (IAM são muito prevalentes no Brasil, e informações sobre estas patologias são bastante procuradas na internet. Por isso, buscamos avaliar a qualidade da informação relacionada com estas doenças disponível em português na internet. MÉTODOS: Foram selecionadas as primeiras 20 páginas em português de cada uma das doenças escolhidas, através do algoritmo de busca do Google®. Como ferramentas para avaliar a qualidade da informação foram utilizados o Discern Questionnarie (DQ e o Health on Net (HON. Para saber se as informações eram adequadas, foram utilizadas as diretrizes (guidelines brasileiras e internacionais para as diferentes comorbidades. RESULTADOS: Ao avaliar o conteúdo das informações disponíveis, 45%, 95%, 85% das páginas continham, respectivamente, a definição de DM, HAS e IAM. Com relação ao diagnóstico e ao tratamento, somente 25% das páginas das três comorbidades apresentavam especificamente esta informação. Somente 15%, 20% e 10% das páginas tinham a certificação pelo HON, respectivamente. Em função do DQ, as páginas obtiveram notas maiores que 50% em 70% das páginas de DM, 65% nas de HAS e 55% nas de IAM. CONCLUSÃO: A informação disponível em português na internet sobre as três patologias escolhidas (DM, HAS e IAM é frequentemente inadequada e insuficiente.OBJECTIVE: Patients and their relatives often look for information about their diseases on the internet. Diabetes mellitus (DM, systemic arterial hypertension (SAH, and acute myocardial infarction (AMI are the most prevalent in Brazil, thus, information on these pathologies is extremely searched for on the internet. For this reason, this study attempted to evaluate the quality of information available in Portuguese on the web

  3. CLAWS: uma ferramenta colaborativa para apoio à interação de surdos com páginas da web.

    OpenAIRE

    Stefan José Oliveira Martins

    2012-01-01

    Os deficientes auditivos têm uma forma peculiar de uso das tecnologias de informação e comunicação (TICs). Como grupo de usuários, essas pessoas nem sempre são consideradas como usuários nos modelos de interação de sistemas comerciais. O objetivo desta pesquisa é conceber uma ferramenta adequada às necessidades dos surdos, que os apoie na interação com páginas da web. Para isso, estudaram-se as particularidades da comunidade surda com as TICs e as barreiras encontradas durante a interação. Co...

  4. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated RAS oncogene and SV40 T-antigen

    Energy Technology Data Exchange (ETDEWEB)

    Su, L.-N.; Little, J.B. (Harvard School of Public Health, Boston, MA (United States))

    1992-08-01

    Three normal human diploid cell strains were transfected with an activated Ha-ras oncogene (EJ ras) or SV40 T-antigen. Multiple clones were examined for morphological alterations, growth requirements, ability to grow under anchorage independent conditions, immortality and tumorigenicity in nude mice. Clones expressing SV40 T-antigen alone or in combination with ras protein p21 were significantly radioresistant as compared with their parent cells or clones transfected with the neo gene only. This radioresistant phenotype persisted in post-crisis, immortalized cell lines. These data suggest that expression of the SV40 T-antigen but not activated Ha-ras plays an important role in the radiosensitivity of human diploid cells. The radioresistant phenotype in SV40 T transfected cells was not related to the enhanced level of genetic instability seen in pre-crisis and newly immortalized cells, nor to the process of immortalization itself. (author).

  5. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated RAS oncogene and SV40 T-antigen

    International Nuclear Information System (INIS)

    Su, L.-N.; Little, J.B.

    1992-01-01

    Three normal human diploid cell strains were transfected with an activated Ha-ras oncogene (EJ ras) or SV40 T-antigen. Multiple clones were examined for morphological alterations, growth requirements, ability to grow under anchorage independent conditions, immortality and tumorigenicity in nude mice. Clones expressing SV40 T-antigen alone or in combination with ras protein p21 were significantly radioresistant as compared with their parent cells or clones transfected with the neo gene only. This radioresistant phenotype persisted in post-crisis, immortalized cell lines. These data suggest that expression of the SV40 T-antigen but not activated Ha-ras plays an important role in the radiosensitivity of human diploid cells. The radioresistant phenotype in SV40 T transfected cells was not related to the enhanced level of genetic instability seen in pre-crisis and newly immortalized cells, nor to the process of immortalization itself. (author)

  6. Measurement Invariance of the Short Version of the Problematic Mobile Phone Use Questionnaire (PMPUQ-SV across Eight Languages

    Directory of Open Access Journals (Sweden)

    Olatz Lopez-Fernandez

    2018-06-01

    Full Text Available The prevalence of mobile phone use across the world has increased greatly over the past two decades. Problematic Mobile Phone Use (PMPU has been studied in relation to public health and comprises various behaviours, including dangerous, prohibited, and dependent use. These types of problematic mobile phone behaviours are typically assessed with the short version of the Problematic Mobile Phone Use Questionnaire (PMPUQ–SV. However, to date, no study has ever examined the degree to which the PMPU scale assesses the same construct across different languages. The aims of the present study were to (i determine an optimal factor structure for the PMPUQ–SV among university populations using eight versions of the scale (i.e., French, German, Hungarian, English, Finnish, Italian, Polish, and Spanish; and (ii simultaneously examine the measurement invariance (MI of the PMPUQ–SV across all languages. The whole study sample comprised 3038 participants. Descriptive statistics, correlations, and Cronbach’s alpha coefficients were extracted from the demographic and PMPUQ-SV items. Individual and multigroup confirmatory factor analyses alongside MI analyses were conducted. Results showed a similar pattern of PMPU across the translated scales. A three-factor model of the PMPUQ-SV fitted the data well and presented with good psychometric properties. Six languages were validated independently, and five were compared via measurement invariance for future cross-cultural comparisons. The present paper contributes to the assessment of problematic mobile phone use because it is the first study to provide a cross-cultural psychometric analysis of the PMPUQ-SV.

  7. A guide to the translation of the Global Initiative for Asthma (GINA) strategy into improved care

    DEFF Research Database (Denmark)

    Boulet, Louis-Philippe; FitzGerald, J Mark; Levy, Mark L

    2012-01-01

    In 1995, the Global Initiative for Asthma (GINA) published an evidence-based workshop report as a guide to clinicians managing asthma patients, and has updated it annually to ensure that recommendations remain current. Although the report has been widely disseminated and influenced clinical...... practice and research, its major objective, of forming the basis for local and national initiatives to improve services for asthma patients, remains to be achieved. Over recent years, the science of guideline implementation has progressed, and encouraging examples of successful asthma programmes have been...... published. This report is intended to draw on this experience and assist with the translation of asthma guideline recommendations into quality programmes for patients with asthma using current knowledge translation principles. It also provides examples of successful initiatives in various socioeconomic...

  8. Effektregnskab for projektet Digitales ll – Det svære valg

    DEFF Research Database (Denmark)

    Mark, Stine

    Effektregnskabet for projektet Digitales ll – Det svære valg er udarbejdet af forskningscenteret INCEVIDA, Aalborg Universitet 2012. Rapporten er gennemført i et samarbejde mellem INCEVIDA, KulturarvNord og Bangsbo Museum. Effektregnskabet tager udgangspunkt i en model for oplevelsesøkonomisk...

  9. SV40 large T-p53 complex: evidence for the presence of two immunologically distinct forms of p53

    International Nuclear Information System (INIS)

    Milner, J.; Gamble, J.

    1985-01-01

    The transforming protein of SV40 is the large T antigen. Large T binds a cellular protein, p53, which is potentially oncogenic by virtue of its functional involvement in the control of cell proliferation. This raises the possibility that p53 may mediate, in part, the transforming function of SV40 large T. Two immunologically distinct forms of p53 have been identified in normal cells: the forms are cell-cycle dependent, one being restricted to nondividing cells (p53-Go) and the second to dividing cells (p53-G divided by). The authors have now dissociated and probed the multimeric complex of SV40 large T-p53 for the presence of immunologically distinct forms of p53. Here they present evidence for the presence of p53-Go and p53-G divided by complexed with SV40 large T

  10. Muusikamaailm : Sibeliuse nädal Järvenpääl. Enescu festival Bukarestis. Ukraina tagastab käsikirjad. Nello Santi 70. Gina Cigna lahkus kõrges eas / Priit Kuusk

    Index Scriptorium Estoniae

    Kuusk, Priit, 1938-

    2001-01-01

    Järvenpääl toimus Sibeliuse nädal. Bukarestis toimus George Enescu festival. Ukraina valitsuse otsusega tagastatakse Berliini Gesangsakademie'le nende fondides olnud hulk käsikirjalis Nimekas itaalia ooperidirigent Nello Santi sai 70. Itaalia päritolu prantsuse ooperilaulja Gina Cigna suri 101 aastasena

  11. Inhibition of in vitro SV40 DNA replication by ultraviolet light

    International Nuclear Information System (INIS)

    Gough, G.; Wood, R.W.

    1989-01-01

    Ultraviolet light-induced DNA damage was found to inhibit SV40 origin-dependent DNA synthesis carried out by soluble humancell extracts. Replication of SV40-based plasmids was reduced to approx. 35% of that in unirradiated controls after irradiation with 50-100 J/m 2 germicidal ultraviolet light, where an average of 3-6 pyrimidine dimer photoproducts were formed per plasmid circle. Inhibition of the DNA helicase activity of T antigen (required for initiation of replication in the in vitro system) was also investigated, and was only significant after much higher fluences, 1000-5000 J/m 2 . The data indicate that DNA damage by ultraviolet light inhibits DNA synthesis in cell-free extracts principally by affecting components of the replication complex other than the DNA helicase activity of T antigen. The soluble system could be used to biochemically investigate the possible bypass or tolerance of DNA damage during replication (author). 21 refs.; 2 figs

  12. Hyperacetylation and differential deacetylation of histones H4 and H3 define two distinct classes of acetylated SV40 chromosomes early in infection

    International Nuclear Information System (INIS)

    Milavetz, Barry

    2004-01-01

    SV40 chromosomes undergoing encapsidation late in infection and SV40 chromatin in virions are hyperacetylated on histones H4 and H3. However, the fate of the SV40 chromosomes containing hyperacetylated histones in a subsequent round of infection has not been determined. In order to determine if SV40 chromosomes undergo changes in the extent of histone acetylation during early infection, we have analyzed SV40 chromosomes isolated 30 min and 3 h postinfection by quantitative ChIP assays, depletion ChIP assays, competitive ChIP assays, and ChIP assays combined with restriction endonuclease sensitivity using antibodies to hyperacetylated histones H4 and H3. We have shown that at 30 min postinfection, the hyperacetylated histones are associated with two distinct classes of SV40 chromosomes. One form is hyperacetylated specifically on histone H4 while a second form is hyperacetylated on both H4 and H3. Both forms of chromosomes appear to contain a nucleosome-free promoter region. Over the course of the next few hours of infection, the class of SV40 chromosomes hyperacetylated on only H4 is reduced or completely eliminated through deacetylation

  13. Título da página electrónica: Proyecto Desaparecidos. Por la memória y la justicia

    Directory of Open Access Journals (Sweden)

    António Sousa Ribeiro

    2012-10-01

    Full Text Available O Projecto Desaparecidos, animado por diversos organismos e activistas de direitos humanos, tem por objectivo preservar o direito à memória das vítimas do terrorismo de Estado na América Latina e no mundo. Contém informações pormenorizadas, não apenas sobre as vítimas, mas também sobre os perpetradores, responsáveis por sequestros, assassínios, actos de tortura e um sem‑número de violações dos direitos humanos nas últimas décadas um pouco por todo o mundo. A página, disponível também em versã...

  14. The suitability of 129SvEv mice for studying depressive-like behaviour: both males and females develop learned helplessness.

    Science.gov (United States)

    Chourbaji, Sabine; Pfeiffer, Natascha; Dormann, Christof; Brandwein, Christiane; Fradley, Rosa; Sheardown, Malcolm; Gass, P

    2010-07-29

    Behavioural studies using transgenic techniques in mice usually require extensive backcrossing to a defined background strain, e.g. to C57BL/6. In this study we investigated whether backcrossing can be replaced by using the 129SvEv strain from which the embryonic stem cells are generally obtained for gene targeting strategies to analyze e.g. depression-like behaviour. For that purpose we subjected male and female 129SvEv mice to two frequently used depression tests and compared them with commonly used C57BL/6 mice. 129SvEv and C57BL/6 mice exhibited differing profiles with regard to locomotion and pain sensitivity. However, in the learned helplessness paradigm, a procedure, which represents a valid method to detect depressive-like behaviour, 129SvEv animals develop a similar level of helplessness as C57BL/6 mice. One great advantage of the 129SvEv animals though, is the fact that in this strain even females develop helplessness, which could not be produced in C57BL/6 mice. In the tail suspension test, both genders of 129SvEv exhibited more despair behaviour than C57BL/6 animals. We therefore suggest that this strain may be utilized in the establishment of new test procedures for affective diseases, since costly and time-consuming backcrossing can be prevented, depressive-like behaviour may be analyzed effectively, and gender-specific topics could be addressed in an adequate way. Copyright 2010 Elsevier B.V. All rights reserved.

  15. Epilepsy caused by an abnormal alternative splicing with dosage effect of the SV2A gene in a chicken model.

    Directory of Open Access Journals (Sweden)

    Marine Douaud

    Full Text Available Photosensitive reflex epilepsy is caused by the combination of an individual's enhanced sensitivity with relevant light stimuli, such as stroboscopic lights or video games. This is the most common reflex epilepsy in humans; it is characterized by the photoparoxysmal response, which is an abnormal electroencephalographic reaction, and seizures triggered by intermittent light stimulation. Here, by using genetic mapping, sequencing and functional analyses, we report that a mutation in the acceptor site of the second intron of SV2A (the gene encoding synaptic vesicle glycoprotein 2A is causing photosensitive reflex epilepsy in a unique vertebrate model, the Fepi chicken strain, a spontaneous model where the neurological disorder is inherited as an autosomal recessive mutation. This mutation causes an aberrant splicing event and significantly reduces the level of SV2A mRNA in homozygous carriers. Levetiracetam, a second generation antiepileptic drug, is known to bind SV2A, and SV2A knock-out mice develop seizures soon after birth and usually die within three weeks. The Fepi chicken survives to adulthood and responds to levetiracetam, suggesting that the low-level expression of SV2A in these animals is sufficient to allow survival, but does not protect against seizures. Thus, the Fepi chicken model shows that the role of the SV2A pathway in the brain is conserved between birds and mammals, in spite of a large phylogenetic distance. The Fepi model appears particularly useful for further studies of physiopathology of reflex epilepsy, in comparison with induced models of epilepsy in rodents. Consequently, SV2A is a very attractive candidate gene for analysis in the context of both mono- and polygenic generalized epilepsies in humans.

  16. Alterations of DNA content in human endometrial stromal cells transfected with a temperature-sensitive SV40: tetraploidization and physiological consequences.

    Science.gov (United States)

    Rinehart, C A; Mayben, J P; Butler, T D; Haskill, J S; Kaufman, D G

    1992-01-01

    The normal genomic stability of human cells is reversed during neoplastic transformation. The SV40 large T antigen alters the DNA content in human endometrial stromal cells in a manner that relates to neoplastic progression. Human endometrial stromal cells were transfected with a plasmid containing the A209 temperature-sensitive mutant of SV40 (tsSV40), which is also defective in the viral origin of replication. Ninety-seven clonal transfectants from seven different primary cell strains were isolated. Initial analysis revealed that 20% of the clonal populations (19/97) had an apparent diploid DNA content, 35% (34/97) had an apparent tetraploid DNA content, and the remainder were mixed populations of diploid and tetraploid cells. No aneuploid populations were observed. Diploid tsSV40 transformed cells always give rise to a population of cells with a tetraploid DNA content when continuously cultured at the permissive temperature. The doubling of DNA content can be vastly accelerated by the sudden reintroduction of large T antigen activity following a shift from non-permissive to permissive temperature. Tetraploid tsSV40 transfected cells have a lower capacity for anchorage-independent growth and earlier entry into 'crisis' than diploid cells. These results indicate that during the pre-crisis, extended lifespan phase of growth, the SV40 large T antigen causes a doubling of DNA content. This apparent doubling of DNA content does not confer growth advantage during the extended lifespan that precedes 'crisis'.

  17. Electrochemical cleaning of Sv-08G2S wire surface

    International Nuclear Information System (INIS)

    Kozlov, E.I.; Degtyarev, V.G.; Novikov, M.P.

    1981-01-01

    Results of industrial tests of the Sv-08G2S wire with different state of surface fwith technological lubrication, after mechanical cleaning, with electrochemically cleaned surface) are presented. Advantages of welding-technological properties of the wire with electroe chemically cleaned surface are shown. An operation principle of the electrochemical cleaning facility is described. A brief specf ification f of the facility is given [ru

  18. Estudio de metales pesados y arsénico en los suelos de olivar de Sierra Mágina, Jaén (España Study of heavy metal and arsenic concentrations in olive farm soils, Sierra Mágina, Jaen, Spain

    Directory of Open Access Journals (Sweden)

    Juan Carlos Menjivar Flores

    2009-10-01

    Full Text Available En Sierra Mágina, Jaén (España fueron seleccionados 35 suelos dedicados al cultivo del olivar en los que se estudió la concentración de cromo (Cr, níquel (Ni, cobre (Cu, plomo (Pb, Zinc (Zn y arsénico (As a profundidades entre 0 - 20 y 20 - 40 cm. Estos suelos se desarrollan sobre materiales carbonatados, predominando Calcisoles pétricos y Regosoles calcáricos (FAO, 1999. La homogeneidad del material parental es responsable de la ausencia de variaciones significativas en las propiedades de los suelos a las profundidades estudiadas, así como en el contenido de metales pesados y arsénico. El Cu es el único elemento que varía con la profundidad (p The content of heavy metals and arsenic was studied in 35 soils under olive cultivation in the Sierra Mágina, Jaen, Spain. The concentrations of chromium (Cr, nickel (Ni, copper (Cu, lead (Pb, zinc (Zn and arsenic (As were determined at depths between 0 - 20 and 20- 40 cm. These soils developed on carbonate materials, predominantly Petric Calcisols and Calcaric Regosols (FAO, 1999. The homogeneity of parental material was responsible for the lack of significant variation seen in soil properties, and alsoheavy metal and arsenic content at the depths studied. Copper was the only element that varied over depth (p<0.01, with a higher mean value between 0 - 20 cm; a consequence of agrochemical applications in the region. Soils with a clay and silt texture had higher concentrations of Cr and Ni, while the rest of the metals, and As, did not present significant variation with the main physical chemical properties of the soil. The studied elements did not exceed the reference levels established by the Andalucia Authorities, presenting similar concentrations to those found in equivalent rocks and in non-contaminated soils. The mean values for As, Cr, Cu, Ni, Pb and Zn were related to the concentrations inherited from the lithological material fromwhich the soils are derived

  19. Effect of caffeine on the ultraviolet light induction of SV40 virus from transformed hamster cells

    International Nuclear Information System (INIS)

    Zamansky, G.B.; Kleinman, L.F.; Little, J.B.; Black, P.H.; Kaplan, J.C.

    1976-01-01

    The effect of caffeine on the uv light induction of SV40 virus from two transformed hamster cell lines heterogeneous for the induction of infectious virus was studied. The amount of virus induced was significantly increased in both cell lines when exposure to uv light was followed by treatment with caffeine. Caffeine in the absence of uv irradiation did not stimulate virus induction, nor did it stimulate SV40 replication in a lytic infection. There was an apparent difference in the concentrations of caffeine which maximally stimulated SV40 virus induction in the two cell lines. This effect could not be explained by differences in cell survival after exposure to uv light and caffeine. Since caffeine is known to cause the accumulation of gaps formed in DNA during postreplication repair of uv-irradiated rodent cells, our results support the hypothesis that the formation of gaps or breaks in DNA is an important early step in virus induction

  20. Časopis "Módní svět" a "Lada" v kulturním kontextu

    OpenAIRE

    Šrolová, Kristýna

    2010-01-01

    The thesis discusses tbe fashion magazine Módní svět (Fashion World) and its enclosure Lada, which it implants into their cultural and historica1 context. The magazine Módní svět (Fashion World) was puhli,hed from 1875 to 1935 and thi, a1,o deline, lhe time circmnscription of tbe themes in tbe thesis. It contextua11y deals with tbe women society during tbe tum of 19th to 20th century, with women's emancipatory movement, with tbe beginning of publishing of rn.agazines for women and fashion mag...

  1. A high-quality narrow passband filter for elastic SV waves via aligned parallel separated thin polymethylmethacrylate plates

    OpenAIRE

    Jun Zhang; Yaolu Liu; Wensheng Yan; Ning Hu

    2017-01-01

    We designed a high-quality filter that consists of aligned parallel polymethylmethacrylate (PMMA) thin plates with small gaps for elastic SV waves propagate in metals. Both the theoretical model and the full numerical simulation show the transmission spectrum of the elastic SV waves through such a filter has several sharp peaks with flawless transmission within the investigated frequencies. These peaks can be readily tuned by manipulating the geometry parameters of the PMMA plates. Our invest...

  2. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with SV40 T-antigen mutants defective in RB and P53 binding domains

    International Nuclear Information System (INIS)

    LingNah Su; Little, J.B.

    1992-01-01

    A series of human diploid fibroblast cell clones were developed by DNA transfection with either wild-type SV40 T-antigen (SV40T) or T-antigen mutants defective in its various functional domains. Cell clones expressing the wild-type SV40 T were significantly radioresistant as compared with clones transfected with the neo gene only (D o 192 ± 13 vs 127 ± 19). This radioresistance persisted in post-crisis, immortalized cell lines. A series of mutants with point or deletion mutations within each functionally active domain of SV40 T were also examined for their ability to alter radiosensitivity and induce morphological transformation. Cell clones transfected with T-antigen mutants defective in nuclear localization or origin binding showed increased radioresistance similar to clones transfected with wild-type T-antigen, and expressed morphological changes characteristic of SV40 T-transfected cells. (author)

  3. Medicina herbal china ofertada en páginas web en español: calidad de la información y riesgos

    OpenAIRE

    Noelia Tejedor-García; Coral García-Pastor; Selma Benito-Martínez; Francisco Javier de Lucio-Cazaña

    2018-01-01

    Objetivo: El auge de la venta en Internet propicia el acceso a productos naturales potencialmente tóxicos y la rápida difusión de la información, no necesariamente veraz, que los proveedores ofrecen al consumidor acerca de sus propiedades. El objetivo del presente trabajo ha sido analizar en páginas web en español de venta de medicina herbal china la calidad de la información ofrecida al consumidor y los posibles riesgos derivados de su consumo. Métodos: i) Búsqueda en Google España de sit...

  4. Crystal Structure of Botulinum Neurotoxin A2 in Complex with the Human Protein Receptor SV2C Reveals Plasticity in Receptor Binding

    Directory of Open Access Journals (Sweden)

    Robert Gustafsson

    2018-04-01

    Full Text Available Botulinum neurotoxins (BoNTs are a family of highly dangerous bacterial toxins, with seven major serotypes (BoNT/A-G. Members of BoNTs, BoNT/A1 and BoNT/B1, have been utilized to treat an increasing number of medical conditions. The clinical trials are ongoing for BoNT/A2, another subtype of BoNT/A, which showed promising therapeutic properties. Both BoNT/A1 and BoNT/A2 utilize three isoforms of synaptic vesicle protein SV2 (SV2A, B, and C as their protein receptors. We here present a high resolution (2.0 Å co-crystal structure of the BoNT/A2 receptor-binding domain in complex with the human SV2C luminal domain. The structure is similar to previously reported BoNT/A-SV2C complexes, but a shift of the receptor-binding segment in BoNT/A2 rotates SV2C in two dimensions giving insight into the dynamic behavior of the interaction. Small differences in key residues at the binding interface may influence the binding to different SV2 isoforms, which may contribute to the differences between BoNT/A1 and BoNT/A2 observed in the clinic.

  5. A physical model study of the travel times and conversion point locations of P-SV converted waves in vertical transversely isotropic media

    Science.gov (United States)

    Tseng, C.

    2013-12-01

    In exploration seismology, subsurface medium commonly exhibits anisotropy, characterized by a vertical transversely isotropic (VTI) model. Due to the need of exploring small reservoirs in complex structures, the seismic exploration is extended to deal with anisotropic media. The P-S converted wave seismic exploration is a relatively inexpensive, broadly applicable, and effective way to obtain the S-wave information of the medium. In anisotropic traveltime analysis, the moveout curve of horizontal P-SV event can help to determine the ratio of the P- and SV-wave vertical velocities, the normal moveout (NMO) velocity of SV-waves, and the anisotropy parameters. The P-SV conversion point (CP) location is of great importance to P-SV data binning, NMO corrections and common conversion point (CCP) stacking, and the anisotropy has a more significant effect on the conversion point location than on the moveout. In this study, we attempt to inspect the theoretical non-hyperbolic moveout and CP equations for the P-SV waves reflected from a VTI layer by numerical calculations and physical modeling. We are also interested in visualizing the variations of the conversion point locations from a designed VTI medium. In traveltime analysis, the theoretical moveout curve is accurate up to offsets about one and a half times the reflector depth (x/z=1.5). However, the moveout curve computed by Fermat's principle fits well to the physical data. The CP locations of P-SV waves are similar to those calculated by Fermat's principle and theoretical CP equation, which are verified by the physical modeling.

  6. Methionine sulfoximine supplementation enhances productivity in GS-CHOK1SV cell lines through glutathione biosynthesis.

    Science.gov (United States)

    Feary, Marc; Racher, Andrew J; Young, Robert J; Smales, C Mark

    2017-01-01

    In Lonza Biologics' GS Gene Expression System™, recombinant protein-producing GS-CHOK1SV cell lines are generated by transfection with an expression vector encoding both GS and the protein product genes followed by selection in MSX and glutamine-free medium. MSX is required to inhibit endogenous CHOK1SV GS, and in effect create a glutamine auxotrophy in the host that can be complemented by the expression vector encoded GS in selected cell lines. However, MSX is not a specific inhibitor of GS as it also inhibits the activity of GCL (a key enzyme in the glutathione biosynthesis pathway) to a similar extent. Glutathione species (GSH and GSSG) have been shown to provide both oxidizing and reducing equivalents to ER-resident oxidoreductases, raising the possibility that selection for transfectants with increased GCL expression could result in the isolation of GS-CHOKISV cell lines with improved capacity for recombinant protein production. In this study we have begun to address the relationship between MSX supplementation, the amount of intracellular GCL subunit and mAb production from a panel of GS-CHOK1SV cell lines. We then evaluated the influence of reduced GCL activity on batch culture of an industrially relevant mAb-producing GS-CHOK1SV cell line. To the best of our knowledge, this paper describes for the first time the change in expression of GCL subunits and recombinant mAb production in these cell lines with the degree of MSX supplementation in routine subculture. Our data also shows that partial inhibition of GCL activity in medium containing 75 µM MSX increases mAb productivity, and its more specific inhibitor BSO used at a concentration of 80 µM in medium increases the specific rate of mAb production eight-fold and the concentration in harvest medium by two-fold. These findings support a link between the inhibition of glutathione biosynthesis and recombinant protein production in industrially relevant systems and provide a process-driven method for

  7. Når det er svært at være ung i DK

    DEFF Research Database (Denmark)

    Nielsen, Jens Christian; Sørensen, Niels Ulrik; Grubb, Ane

    I rapporten Når det er svært at være ung i DK – unges beretninger om mistrivsel og ungdomsliv præsenteres resultaterne af et kvalitativt studie af mistrivsel og ungdomsliv blandt 15-24-årige unge i Danmark. Studiet bygger på dybdegående interviews med 33 unge fra forskellige dele af landet, der...... fortæller om deres erfaringer med diverse mistrivselsformer, som ensomhed, selvskadende adfærd og mobning, og om deres besvær med at håndtere de krav, udfordringer og muligheder, der i øvrigt præger det moderne ungdomsliv. Studiet indgår i det treårige forskningsprojekt Når det er svært at være ung i DK...

  8. Las páginas culturales de los diarios como puente de comunicación con el lector

    Directory of Open Access Journals (Sweden)

    Dra. Margarita Pérez de Eulate Vargas

    1999-01-01

    Full Text Available Es evidente que la prensa juega un papel importante en el engranaje cultural de nuestro mundo, ya que además de contribuir a la formación de la opinión pública, vierte sobre los lectores noticias de toda índole que pueden influir en sus decisiones y negocios personales. La información cultural inserta en los periódicos contribuye de modo destacado a una mayor socialización del público lector y a una elevación del nivel de alfabetización. De las páginas culturales nacen el diálogo y la controversia ideológica que posibilitan el pluralismo y el encuentro con la verdad. Desde ellas también se puede luchar contra la pasividad y convertir la cultura amenazada en algo vivo y eficaz.

  9. Fetal bovine serum and human constitutive androstane receptor: Evidence for activation of the SV23 splice variant by artemisinin, artemether, and arteether in a serum-free cell culture system

    Energy Technology Data Exchange (ETDEWEB)

    Lau, Aik Jiang; Chang, Thomas K.H., E-mail: thomas.chang@ubc.ca

    2014-06-01

    The naturally occurring SV23 splice variant of human constitutive androstane receptor (hCAR-SV23) is activated by di-(2-ethylhexyl)phthalate (DEHP), which is detected as a contaminant in fetal bovine serum (FBS). In our initial experiment, we compared the effect of dialyzed FBS, charcoal-stripped, dextran-treated FBS (CS-FBS), and regular FBS on the basal activity and ligand-activation of hCAR-SV23 in a cell-based reporter gene assay. In transfected HepG2 cells cultured in medium supplemented with 10% FBS, basal hCAR-SV23 activity varied with the type of FBS (regular > dialyzed > CS). DEHP increased hCAR-SV23 activity when 10% CS-FBS, but not regular FBS or dialyzed FBS, was used. With increasing concentrations (1–10%) of regular FBS or CS-FBS, hCAR-SV23 basal activity increased, whereas in DEHP-treated cells, hCAR-SV23 activity remained similar (regular FBS) or slightly increased (CS-FBS). Subsequent experiments identified a serum-free culture condition to detect DEHP activation of hCAR-SV23. Under this condition, artemisinin, artemether, and arteether increased hCAR-SV23 activity, whereas they decreased it in cells cultured in medium supplemented with 10% regular FBS. By comparison, FBS increased the basal activity of the wild-type isoform of hCAR (hCAR-WT), whereas it did not affect the basal activity of the SV24 splice variant (hCAR-SV24) or ligand activation of hCAR-SV24 and hCAR-WT by 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO). The use of serum-free culture condition was suitable for detecting CITCO activation of hCAR-WT and hCAR-SV24. In conclusion, FBS leads to erroneous classification of pharmacological ligands of hCAR-SV23 in cell-based assays, but investigations on functional ligands of hCAR isoforms can be conducted in serum-free culture condition. - Highlights: • FBS leads to erroneous pharmacological classification of hCAR-SV23 ligands. • Artemisinin, artemether, and arteether activate hCAR-SV

  10. Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains

    Directory of Open Access Journals (Sweden)

    Taavi Vanaveski

    2018-06-01

    Full Text Available The main goal of the study was to characterize the behavioral and metabolomic profiles of repeated administration (for 11 days of d-amphetamine (AMPH, 3 mg/kg i. p., indirect agonist of dopamine (DA, in widely used 129S6/SvEvTac (129Sv and C57BL/6NTac (Bl6 mouse strains. Acute administration of AMPH (acute AMPH induced significantly stronger motor stimulation in Bl6. However, repeated administration of AMPH (repeated AMPH caused stronger motor sensitization in 129Sv compared acute AMPH. Body weight of 129Sv was reduced after repeated saline and AMPH, whereas no change occurred in Bl6. In the metabolomic study, acute AMPH induced an elevation of isoleucine and leucine, branched chain amino acids (BCAA, whereas the level of hexoses was reduced in Bl6. Both BCAAs and hexoses remained on level of acute AMPH after repeated AMPH in Bl6. Three biogenic amines [asymmetric dimethylarginine (ADMA, alpha-aminoadipic acid (alpha-AAA, kynurenine] were significantly reduced after repeated AMPH. Acute AMPH caused in 129Sv a significant reduction of valine, lysophosphatidylcholines (lysoPC a C16:0, lysoPC a C18:2, lysoPC a C20:4, phosphatidylcholine (PC diacyls (PC aa C34:2, PC aa C36:2, PC aa C36:3, PC aa C36:4 and alkyl-acyls (PC ae C38:4, PC ae C40:4. However, repeated AMPH increased the levels of valine and isoleucine, long-chain acylcarnitines (C14, C14:1-OH, C16, C18:1, PC diacyls (PC aa C38:4, PC aa C38:6, PC aa C42:6, PC acyl-alkyls (PC ae C38:4, PC ae C40:4, PC ae C40:5, PC ae C40:6, PC ae C42:1, PC ae C42:3 and sphingolipids [SM(OHC22:1, SM C24:0] compared to acute AMPH in 129Sv. Hexoses and kynurenine were reduced after repeated AMPH compared to saline in 129Sv. The established changes probably reflect a shift in energy metabolism toward lipid molecules in 129Sv because of reduced level of hexoses. Pooled data from both strains showed that the elevation of isoleucine and leucine was a prominent biomarker of AMPH-induced behavioral sensitization

  11. ART-GUMENTACIÓN EN UN DISCURSO TURÍSTICO VIRTUAL. MADRID EN LA PÁGINA WEB DE TURESPAÑA: SPAIN.INFO

    Directory of Open Access Journals (Sweden)

    Laurence Chapuis

    2016-01-01

    Full Text Available Turespaña es un organismo creado con el propósito de acometer acciones en el exterior para la promoción de España como destino turístico, por lo que era fundamental que tuviera una persuasiva presencia en Internet. Basándonos en el análisis argumentativo de Amossy, estudiamos la estrategia discursiva desarrollada en la página de Madrid del portal de Spain.info para convencer al visitante vir - tual de viajar a la capital. Así, en ese contexto digital, mostramos cómo el locutor construye su imagen (ethos y la de su destinatario, y maneja las relaciones entre ambos para cumplir con sus objetivos.

  12. Når det er svært at være ung i DK

    DEFF Research Database (Denmark)

    Nielsen, Jens Christian; Sørensen, Niels Ulrik

    Når det er svært at være ung i DK – viden og råd om unges trivsel og mistrivsel er afslutningen på et større forskningsprojekt om unges trivsel og mistrivsel. Hæftet præsenterer forskningsprojektets hovedkonklusioner og giver desuden en række råd og ideer til, hvordan voksne kan hjælpe unge med...... at håndtere mistrivsel. Hæftet er udarbejdet af forskerne Jens Christian Nielsen og Niels Ulrik Sørensen fra Center for Ungdomsforskning. Det er den sidste publikation i forskningsprojektet Når det er svært at være ung i DK, der fra 2008-2011 har belyst unges trivsel og mistrivsel. Projektet bygger både på en...

  13. Når det er svært at være ung i DK

    DEFF Research Database (Denmark)

    Nielsen, Jens Christian; Sørensen, Niels Ulrik; Ozmec, Martha Nina

    I rapporten Når det er svært at være ung i DK – unges trivsel og mistrivsel i tal offentliggør Center for Ungdomsforskning resultaterne af en stor videnskabelig spørgeskemaundersøgelse om trivsel og mistrivsel blandt 15-24-årige unge i Danmark. Rapporten indgår i det treårige forskningsprojekt Når...... det er svært at være ung i DK, som Center for Ungdomsforskning udfører med støtte fra Egmont Fonden. Rapporten bygger på telefoninterviews med 3.481 unge, der udgør et repræsentativt udsnit af alle 15-24-årige unge i Danmark. I rapporten forfølges de unges trivsel og mistrivsel gennem to spor: 1) Et...

  14. Reconstitution of wild type viral DNA in simian cells transfected with early and late SV40 defective genomes.

    Science.gov (United States)

    O'Neill, F J; Gao, Y; Xu, X

    1993-11-01

    The DNAs of polyomaviruses ordinarily exist as a single circular molecule of approximately 5000 base pairs. Variants of SV40, BKV and JCV have been described which contain two complementing defective DNA molecules. These defectives, which form a bipartite genome structure, contain either the viral early region or the late region. The defectives have the unique property of being able to tolerate variable sized reiterations of regulatory and terminus region sequences, and portions of the coding region. They can also exchange coding region sequences with other polyomaviruses. It has been suggested that the bipartite genome structure might be a stage in the evolution of polyomaviruses which can uniquely sustain genome and sequence diversity. However, it is not known if the regulatory and terminus region sequences are highly mutable. Also, it is not known if the bipartite genome structure is reversible and what the conditions might be which would favor restoration of the monomolecular genome structure. We addressed the first question by sequencing the reiterated regulatory and terminus regions of E- and L-SV40 DNAs. This revealed a large number of mutations in the regulatory regions of the defective genomes, including deletions, insertions, rearrangements and base substitutions. We also detected insertions and base substitutions in the T-antigen gene. We addressed the second question by introducing into permissive simian cells, E- and L-SV40 genomes which had been engineered to contain only a single regulatory region. Analysis of viral DNA from transfected cells demonstrated recombined genomes containing a wild type monomolecular DNA structure. However, the complete defectives, containing reiterated regulatory regions, could often compete away the wild type genomes. The recombinant monomolecular genomes were isolated, cloned and found to be infectious. All of the DNA alterations identified in one of the regulatory regions of E-SV40 DNA were present in the recombinant

  15. SV40 Utilizes ATM Kinase Activity to Prevent Non-homologous End Joining of Broken Viral DNA Replication Products

    Science.gov (United States)

    Sowd, Gregory A.; Mody, Dviti; Eggold, Joshua; Cortez, David; Friedman, Katherine L.; Fanning, Ellen

    2014-01-01

    Simian virus 40 (SV40) and cellular DNA replication rely on host ATM and ATR DNA damage signaling kinases to facilitate DNA repair and elicit cell cycle arrest following DNA damage. During SV40 DNA replication, ATM kinase activity prevents concatemerization of the viral genome whereas ATR activity prevents accumulation of aberrant genomes resulting from breakage of a moving replication fork as it converges with a stalled fork. However, the repair pathways that ATM and ATR orchestrate to prevent these aberrant SV40 DNA replication products are unclear. Using two-dimensional gel electrophoresis and Southern blotting, we show that ATR kinase activity, but not DNA-PKcs kinase activity, facilitates some aspects of double strand break (DSB) repair when ATM is inhibited during SV40 infection. To clarify which repair factors associate with viral DNA replication centers, we examined the localization of DSB repair proteins in response to SV40 infection. Under normal conditions, viral replication centers exclusively associate with homology-directed repair (HDR) and do not colocalize with non-homologous end joining (NHEJ) factors. Following ATM inhibition, but not ATR inhibition, activated DNA-PKcs and KU70/80 accumulate at the viral replication centers while CtIP and BLM, proteins that initiate 5′ to 3′ end resection during HDR, become undetectable. Similar to what has been observed during cellular DSB repair in S phase, these data suggest that ATM kinase influences DSB repair pathway choice by preventing the recruitment of NHEJ factors to replicating viral DNA. These data may explain how ATM prevents concatemerization of the viral genome and promotes viral propagation. We suggest that inhibitors of DNA damage signaling and DNA repair could be used during infection to disrupt productive viral DNA replication. PMID:25474690

  16. SV40 utilizes ATM kinase activity to prevent non-homologous end joining of broken viral DNA replication products.

    Directory of Open Access Journals (Sweden)

    Gregory A Sowd

    2014-12-01

    Full Text Available Simian virus 40 (SV40 and cellular DNA replication rely on host ATM and ATR DNA damage signaling kinases to facilitate DNA repair and elicit cell cycle arrest following DNA damage. During SV40 DNA replication, ATM kinase activity prevents concatemerization of the viral genome whereas ATR activity prevents accumulation of aberrant genomes resulting from breakage of a moving replication fork as it converges with a stalled fork. However, the repair pathways that ATM and ATR orchestrate to prevent these aberrant SV40 DNA replication products are unclear. Using two-dimensional gel electrophoresis and Southern blotting, we show that ATR kinase activity, but not DNA-PK(cs kinase activity, facilitates some aspects of double strand break (DSB repair when ATM is inhibited during SV40 infection. To clarify which repair factors associate with viral DNA replication centers, we examined the localization of DSB repair proteins in response to SV40 infection. Under normal conditions, viral replication centers exclusively associate with homology-directed repair (HDR and do not colocalize with non-homologous end joining (NHEJ factors. Following ATM inhibition, but not ATR inhibition, activated DNA-PK(cs and KU70/80 accumulate at the viral replication centers while CtIP and BLM, proteins that initiate 5' to 3' end resection during HDR, become undetectable. Similar to what has been observed during cellular DSB repair in S phase, these data suggest that ATM kinase influences DSB repair pathway choice by preventing the recruitment of NHEJ factors to replicating viral DNA. These data may explain how ATM prevents concatemerization of the viral genome and promotes viral propagation. We suggest that inhibitors of DNA damage signaling and DNA repair could be used during infection to disrupt productive viral DNA replication.

  17. Sigma Virus (DMelSV Incidence in Lines of Drosophila melanogaster Selected for Survival following Infection with Bacillus cereus

    Directory of Open Access Journals (Sweden)

    Meghan L. Bentz

    2017-01-01

    Full Text Available The immune response of Drosophila melanogaster is complex and involves both specific and general responses to parasites. In this study we tested for cross-immunity for bacteria and viruses by scoring the incidence of infection with the vertically transmitted Sigma virus (DMelSV in the progeny of a cross between females transmitting DMelSV at high frequencies and males from lines subjected to three selection regimes related to resistance to Bacillus cereus. There was no significant difference in transmission of DMelSV among selection regimes, though results suggest that the B. cereus selected lines had lower rates of infection by DMelSV. We found a significant difference in viral infection with respect to the sex of the progeny, with males consistently less likely to be infected than females. Given a finite energy budget, flies that have experienced immune system challenge may show alterations in other life history traits. Later eclosing progeny were also less likely to be infected than earlier eclosing progeny, indicating a relationship with development time. Finally, there was a significant interaction between the timing of collection and the sex of the progeny, such that later eclosing males were the most resistant group. Increased development time is sometimes associated with increased energy acquisition; from this perspective, increased development time may be associated with acquiring sufficient resources for effective resistance.

  18. Reply to Comments on Measuring marine iron(III) complexes by CLE-AdSV

    NARCIS (Netherlands)

    Town, R.M.; Leeuwen, van H.P.

    2005-01-01

    The interpretation of CLE-AdSV based iron(iii) speciation data for marine waters has been called into question in light of the kinetic features of the measurement. The implications of the re-think may have consequences for understanding iron biogeochemistry and its impact on ecosystem functioning.

  19. Internet y las nuevas tecnologías: evolución de la página Web del Instituto de estudio de las adicciones

    OpenAIRE

    Jesús A. Lacoste Marín

    2005-01-01

    Se realiza una descripción y valoración del nacimiento y aplicación de las nuevas tecno­logías en el campo de las adicciones, con especial detalle en la página web del Instituto para el Estudio de las Adicciones www.lasdrogas.info. Se analiza la evolución desde su creación y los contenidos que pone a disposición de toda la comunidad profesional y de la sociedad en general como contribución para la atención y prevención de las drogodependencias.

  20. El Cine durante la Primera Guerra Mundia a través de las páginas del diario El Progreso de Tenerife

    OpenAIRE

    Orlando Betancor

    2013-01-01

    Este artículo analiza el tratamiento in­formativo dado a las películas cinematográfi­cas en las páginas del periódico El Progreso de Santa Cruz de Tenerife durante la Primera Guerra Mundial. Además, recoge los princi­pales acontecimientos cinematográficos que tuvieron lugar en Tenerife en este momento y su repercusión en este diario. Investiga el papel de los anuncios publicitarios de los loca­les de exhibición, editados en este periódico, que mostraron al público de la Isla estas obras de ar...

  1. The adaptation of limb kinematics to increasing walking speeds in freely moving mice 129/Sv and C57BL/6.

    Science.gov (United States)

    Serradj, Nadjet; Jamon, Marc

    2009-07-19

    The kinematics of locomotion was analyzed in two strains of great importance for the creation of mutated mice (C56BL/6 and 129/Sv). Different behavioral situations were used to trigger sequences of movement covering the whole range of velocities in the mice, and the variations of kinematic parameters were analyzed in relation with velocity. Both stride frequency and stride length contributed to the moving speed, but stride frequency was found to be the main contributor to the speed increase. A trot-gallop transition was detected at speed about 70 cm/s, in relation with a sharp shift in limb coordination. The results of this study were consistent with pieces of information previously published concerning the gait analyses of other strains, and provided an integrative view of the basic motor pattern of mice. On the other hand some qualitative differences were found in the movement characteristics of the two strains. The stride frequency showed a higher contribution to speed in 129/Sv than in C57BL/6. In addition, 129/Sv showed a phase shift in the forelimb and hindlimb, and a different position of the foot during the stance time that revealed a different gait and body position during walking. Overall, 129/Sv moved at a slower speed than C57BL/6 in any behavioral situation. This difference was related to a basal lower level of motor activity. The possibility that an alteration in the dopamine circuit was responsible for the different movement pattern in 129/Sv is discussed.

  2. Cat odor exposure induces distinct changes in the exploratory behavior and Wfs1 gene expression in C57Bl/6 and 129Sv mice.

    Science.gov (United States)

    Raud, Sirli; Sütt, Silva; Plaas, Mario; Luuk, Hendrik; Innos, Jürgen; Philips, Mari-Anne; Kõks, Sulev; Vasar, Eero

    2007-10-16

    129Sv and C57Bl/6 (Bl6) strains are two most widely used inbred mice strains for generation of transgenic animals. The present study confirms the existence of substantial differences in the behavior of these two mice strains. The exploratory behavior of Bl6 mice in a novel environment was significantly higher compared to 129Sv mice. The exposure of mice to cat odor-induced an anxiety-like state in Bl6, but not in 129Sv mice. The levels of Wfs1 gene expression did not differ in the prefrontal cortex, mesolimbic area and temporal lobe of experimentally naive Bl6 and 129Sv mice. However, after cat odor exposure the expression of Wfs1 gene was significantly lower in the mesolimbic area and temporal lobe of Bl6 mice compared to 129Sv strain. Dynamics of Wfs1 gene expression and exploratory behavior suggest that the down-regulation of Wfs1 gene in Bl6 mice might be related to the increased anxiety. Further studies are needed to test the robustness and possible causal relationship of this finding.

  3. SV40 T antigen alone drives karyotype instability that precedes neoplastic transformation of human diploid fibroblasts.

    Science.gov (United States)

    Ray, F A; Peabody, D S; Cooper, J L; Cram, L S; Kraemer, P M

    1990-01-01

    To define the role of SV40 large T antigen in the transformation and immortalization of human cells, we have constructed a plasmid lacking most of the unique coding sequences of small t antigen as well as the SV40 origin of replication. The promoter for T antigen, which lies within the origin of replication, was deleted and replaced by the Rous sarcoma virus promoter. This minimal construct was co-electroporated into normal human fibroblasts of neonatal origin along with a plasmid containing the neomycin resistance gene (neo). Three G418-resistant, T antigen-positive clones were expanded and compared to three T antigen-positive clones that received the pSV3neo plasmid (capable of expressing large and small T proteins and having two origins of replication). Autonomous replication of plasmid DNA was observed in all three clones that received pSV3neo but not in any of the three origin minus clones. Immediately after clonal expansion, several parameters of neoplastic transformation were assayed. Low percentages of cells in T antigen-positive populations were anchorage independent or capable of forming colonies in 1% fetal bovine serum. The T antigen-positive clones generally exhibited an extended lifespan in culture but rarely became immortalized. Large numbers of dead cells were continually generated in all T antigen-positive, pre-crisis populations. Ninety-nine percent of all T antigen-positive cells had numerical or structural chromosome aberrations. Control cells that received the neo gene did not have an extended life span, did not have noticeable numbers of dead cells, and did not exhibit karyotype instability. We suggest that the role of T antigen protein in the transformation process is to generate genetic hypervariability, leading to various consequences including neoplastic transformation and cell death.

  4. SV-BR-1-GM, a Clinically Effective GM-CSF-Secreting Breast Cancer Cell Line, Expresses an Immune Signature and Directly Activates CD4+ T Lymphocytes

    Directory of Open Access Journals (Sweden)

    Markus D. Lacher

    2018-05-01

    Full Text Available Targeted cancer immunotherapy with irradiated, granulocyte–macrophage colony-stimulating factor (GM-CSF-secreting, allogeneic cancer cell lines has been an effective approach to reduce tumor burden in several patients. It is generally assumed that to be effective, these cell lines need to express immunogenic antigens coexpressed in patient tumor cells, and antigen-presenting cells need to take up such antigens then present them to patient T cells. We have previously reported that, in a phase I pilot study (ClinicalTrials.gov NCT00095862, a subject with stage IV breast cancer experienced substantial regression of breast, lung, and brain lesions following inoculation with clinical formulations of SV-BR-1-GM, a GM-CSF-secreting breast tumor cell line. To identify diagnostic features permitting the prospective identification of patients likely to benefit from SV-BR-1-GM, we conducted a molecular analysis of the SV-BR-1-GM cell line and of patient-derived blood, as well as a tumor specimen. Compared to normal human breast cells, SV-BR-1-GM cells overexpress genes encoding tumor-associated antigens (TAAs such as PRAME, a cancer/testis antigen. Curiously, despite its presumptive breast epithelial origin, the cell line expresses major histocompatibility complex (MHC class II genes (HLA-DRA, HLA-DRB3, HLA-DMA, HLA-DMB, in addition to several other factors known to play immunostimulatory roles. These factors include MHC class I components (B2M, HLA-A, HLA-B, ADA (encoding adenosine deaminase, ADGRE5 (CD97, CD58 (LFA3, CD74 (encoding invariant chain and CLIP, CD83, CXCL8 (IL8, CXCL16, HLA-F, IL6, IL18, and KITLG. Moreover, both SV-BR-1-GM cells and the responding study subject carried an HLA-DRB3*02:02 allele, raising the question of whether SV-BR-1-GM cells can directly present endogenous antigens to T cells, thereby inducing a tumor-directed immune response. In support of this, SV-BR-1-GM cells (which also carry the HLA-DRB3*01:01 allele treated with

  5. Improved AMD counters quicky type to achieve a level of 0.2 log derivative mSv output in counts refills; Mejora de la AMD en contadores tipo quicky para conseguir un nivel derivado de registro de 0,2 mSV en contajes de salida en recargas

    Energy Technology Data Exchange (ETDEWEB)

    Bravp Perez-Tinao, B.; Marchena Gonzalez, P.; Sollet Danudo, E.

    2011-07-01

    To ensure that no worker receives contract exposed by internal exposure dose computed not exceed 1 mSv / year, the Nuclear Security Council requested the Spanish nuclear power plants and Tecnatom that in 2010 would ensure a level derived from registration in internal dosimetry counts refills output equal to or less than 0.2 mSv, which represented a reduction of AMD computers.

  6. SV3R : un framework pour la gestion de la variabilité des services

    Directory of Open Access Journals (Sweden)

    Boutaina Chakir

    2014-11-01

    Full Text Available The emergence and expansion of the development paradigm based on service-oriented approaches have been behind the elaboration of new models and methods that aim at facilitating the reuse of services within multiples context of use. This requires providing systematically services with several possible realizations. Among the promising approaches that achieve this objective is the management of variability which has been widely adopted by the software engineering disciplines and whose objective is to facilitate the adaptation or the configuration of software artifacts in a systematic way. Hence, in this work we provide a framework for the development (for and by reuse of services supporting variability, called “SV3R” (Service Variability Representation and Resolution for Reuse. This paper introduces at first the concept of variability. Afterwards, it presents some related work, before giving an overview of the framework SV3R and describing

  7. StralSV: assessment of sequence variability within similar 3D structures and application to polio RNA-dependent RNA polymerase

    Energy Technology Data Exchange (ETDEWEB)

    Zemla, A; Lang, D; Kostova, T; Andino, R; Zhou, C

    2010-11-29

    Most of the currently used methods for protein function prediction rely on sequence-based comparisons between a query protein and those for which a functional annotation is provided. A serious limitation of sequence similarity-based approaches for identifying residue conservation among proteins is the low confidence in assigning residue-residue correspondences among proteins when the level of sequence identity between the compared proteins is poor. Multiple sequence alignment methods are more satisfactory - still, they cannot provide reliable results at low levels of sequence identity. Our goal in the current work was to develop an algorithm that could overcome these difficulties and facilitate the identification of structurally (and possibly functionally) relevant residue-residue correspondences between compared protein structures. Here we present StralSV, a new algorithm for detecting closely related structure fragments and quantifying residue frequency from tight local structure alignments. We apply StralSV in a study of the RNA-dependent RNA polymerase of poliovirus and demonstrate that the algorithm can be used to determine regions of the protein that are relatively unique or that shared structural similarity with structures that are distantly related. By quantifying residue frequencies among many residue-residue pairs extracted from local alignments, one can infer potential structural or functional importance of specific residues that are determined to be highly conserved or that deviate from a consensus. We further demonstrate that considerable detailed structural and phylogenetic information can be derived from StralSV analyses. StralSV is a new structure-based algorithm for identifying and aligning structure fragments that have similarity to a reference protein. StralSV analysis can be used to quantify residue-residue correspondences and identify residues that may be of particular structural or functional importance, as well as unusual or unexpected

  8. Reflection of P and SV waves at the free surface of a monoclinic ...

    Indian Academy of Sciences (India)

    The propagation of plane waves in an anisotropic elastic medium possessing monoclinic symmetry is discussed. The expressions for the phase velocity of qP and qSV waves propagating in the plane of elastic symmetry are obtained in terms of the direction cosines of the propagation vector. It is shown that, in general, ...

  9. Orwellův svět v roce 2009

    Directory of Open Access Journals (Sweden)

    Miloslav Petrusek

    2017-10-01

    Full Text Available V červnu 1949 vyšla v nakladatelství Harcourt, Brace v New Yorku kniha, která se zařadila mezi několik zásadních beletristických děl 20. století. Svým politickým vyzněním měla otřást totalitními režimy, tímto zrůdným plodem „instrumentální racionality“ a ad absurdum dovedené byrokracie. Pomineme literaturu, jejímž dominantním literárním tématem je kritika nacismu – třebas Feuchtwangerovu triologii, román I ve smrti sami, Mannův esej Bratr Hitler, ale i alegorický příběh Doktora Fausta či nejslavnější román Heinricha Manna Mefisto, případně kritika latinskoamerických autoritářských režimů – třebas vynikající román Marquézův Podzim patriarchy nebo romány Carpentierovy Výbuch v katedrále a Náprava dle metody. Setrváme především u literárního obrazu a kritiky sovětského systému. Je jistě věcí individuální zkušenosti (osobní i literární a do značné míry subjektivní volby, která díla vybereme. Zdá se nicméně, že nejméně tři patří k neopominutelné „klasice“. Jsou totiž nejen formálně často jmenována, ale stále čtena, jsou živá a zřejmě tedy obsahují jakýsi aktualizující náboj, který jim nedovolí zůstat v řadě strnulé nehybnosti nečtených, leč ctěných klasiků. Myslím, že jde o knihu Artura Koestlera Tma o polednách, která již v roce 1939 odhalila – bez znalosti jakýchkoliv svědeckých či archivních podkladů – mechanismus moskevských procesů, které „otřásly světem“, o žánrově science fiction s titulem 1984 George Orwella a konečně o Solženicynův monument Soustroví Gulag.

  10. SV40 late protein VP4 forms toroidal pores to disrupt membranes for viral release.

    Science.gov (United States)

    Raghava, Smita; Giorda, Kristina M; Romano, Fabian B; Heuck, Alejandro P; Hebert, Daniel N

    2013-06-04

    Nonenveloped viruses are generally released from the cell by the timely lysis of host cell membranes. SV40 has been used as a model virus for the study of the lytic nonenveloped virus life cycle. The expression of SV40 VP4 at later times during infection is concomitant with cell lysis. To investigate the role of VP4 in viral release and its mechanism of action, VP4 was expressed and purified from bacteria as a fusion protein for use in membrane disruption assays. Purified VP4 perforated membranes as demonstrated by the release of fluorescent markers encapsulated within large unilamellar vesicles or liposomes. Dynamic light scattering results revealed that VP4 treatment did not cause membrane lysis or change the size of the liposomes. Liposomes encapsulated with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-3-indacene-labeled streptavidin were used to show that VP4 formed stable pores in membranes. These VP4 pores had an inner diameter of 1-5 nm. Asymmetrical liposomes containing pyrene-labeled lipids in the outer monolayer were employed to monitor transbilayer lipid diffusion. Consistent with VP4 forming toroidal pore structures in membranes, VP4 induced transbilayer lipid diffusion or lipid flip-flop. Altogether, these studies support a central role for VP4 acting as a viroporin in the disruption of cellular membranes to trigger SV40 viral release by forming toroidal pores that unite the outer and inner leaflets of membrane bilayers.

  11. Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia

    Directory of Open Access Journals (Sweden)

    C.B. Sacramento

    2010-08-01

    Full Text Available The main objective of the present study was to find suitable DNA-targeting sequences (DTS for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS and hypoxia-responsive element (HRE sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF. The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2 and hypoxia (less than 5% O2 were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by real-time PCR, and the expression level of the hVEGF gene was measured by ELISA. The in vitro nuclear transport efficiency of the SV40-DTS plasmid was about 50% lower under hypoxia, while the HRE plasmid was about 50% higher under hypoxia. Quantitation of reporter gene expression in vitro and in vivo, under hypoxia and normoxia, confirmed that the SV40-DTS plasmid functioned better under normoxia, while the HRE plasmid was superior under hypoxia. These results indicate that the efficiency of gene expression by plasmids containing DNA binding sequences is affected by the concentration of oxygen in the medium.

  12. Ultraviolet radiation inactivates SV40 by disrupting at least four genetic functions

    International Nuclear Information System (INIS)

    Brown, T.C.; Cerutti, P.A.

    1986-01-01

    The most UV sensitive region within the SV40 viral genome contains the transcriptional promotors and enhancers for the early and late viral genes plus part of the origin of DNA replication. Lesions within this regulatory region are 3.2-fold more effective in inactivating viral DNA than is the same amount of damage randomly distributed throughout the viral genome. The region least sensitive to damage lies within the coding portion of the viral coat protein genes, which are expressed only late in infection and would therefore be transcribed from undamaged progeny viral genomes, provided DNA replication occurs. Damage within this region is only 45% as effective in inactivating viral DNA as are randomly distributed lesions. Thus there is a 7-fold difference in the lethal effect of DNA damage within the most and least sensitive regions of the viral genome. Intermediate sensitivities are observed within the transcribed portion of the viral A gene, coding for the T antigen whose expression is required early in infection, and in a region at the terminus of DNA replication. The sum of the individual sensitivities for all regions of the SV40 genome is equal to the total sensitivity of viral DNA subjected to random damage. (author)

  13. Validation of the portuguese version of the tampa scale for kinesiophobia heart (TSK-SV heart

    Directory of Open Access Journals (Sweden)

    Gabriela Lima de Melo Ghisi

    Full Text Available ABSTRACT Introduction: It has been shown that kinesiophobia has a negative influence on the outcomes of cardiac rehabilitation and consequently is important for the clinical setting. Objective: The objective of this study was to translate, culturally adapt, and psychometrically validate the Tampa Scale for Kinesiophobia Heart (TSK-SV Heart to Brazilian Portuguese. Methods: The Portuguese version was tested in 300 patients in cardiac rehabilitation. Test-retest reliability was assessed by intraclass correlation coefficient, internal consistency by Cronbach’s alpha, and criterion validity was assessed with respect to patients’ education, income, duration of cardiac rehabilitation, and sex. Results: After intraclass correlation coefficient analysis, one item was excluded. All four areas were considered internally consistent (α >0.7. Significant differences between mean total scores and income (p 37. Conclusions: The Brazilian Portuguese version of TSK-SV Heart demonstrated sufficient reliability, consistency and validity, supporting its use in future studies.

  14. Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures

    DEFF Research Database (Denmark)

    Tanner, V A; Ploug, Thorkil; Tao-Cheng, J H

    1996-01-01

    substantially improved the efficiency of the preembedding EM ICC procedures for cell cultures. The advantages and related caveats of this method are discussed. SV2 was distinctly localized on dusters of synaptic vesicles and large dense-cored vesicles (LDCV). The distribution of SV2 on these two types...... of secretory vesicles was compared quantitatively to that of another secretory vesicle-associated transmembrane protein, synaptophysin. In cultures under similar experimental conditions, the ratio of SV2 vs synaptophysin ICC staining on synaptic vesicle dusters was about 1:1, whereas it was about 9:1 on LDCV...

  15. 36 http://spilplus.journals.ac.za/

    African Journals Online (AJOL)

    behaviorisme, veral van Watson (Behaviorism, 1924) en ;.leiss (A Theoretical basis of human behaviour, 1924, hersiene uitgawe 1929). Soos bekend, is die behaviorisme die opvatting dat psigologiese verskynsels voldoende behandel en verklaar kan word deur 'n studie van die reaksies van 'n organisme op stimuli sonder ...

  16. SvO(2)-guided resuscitation for experimental septic shock: effects of fluid infusion and dobutamine on hemodynamics, inflammatory response, and cardiovascular oxidative stress.

    Science.gov (United States)

    Rosário, André Loureiro; Park, Marcelo; Brunialti, Milena Karina; Mendes, Marialice; Rapozo, Marjorie; Fernandes, Denise; Salomão, Reinaldo; Laurindo, Francisco Rafael; Schettino, Guilherme Paula; Azevedo, Luciano Cesar P

    2011-12-01

    The pathogenetic mechanisms associated to the beneficial effects of mixed venous oxygen saturation (SvO(2))-guided resuscitation during sepsis are unclear. Our purpose was to evaluate the effects of an algorithm of SvO(2)-driven resuscitation including fluids, norepinephrine and dobutamine on hemodynamics, inflammatory response, and cardiovascular oxidative stress during a clinically resembling experimental model of septic shock. Eighteen anesthetized and catheterized pigs (35-45 kg) were submitted to peritonitis by fecal inoculation (0.75 g/kg). After hypotension, antibiotics were administered, and the animals were randomized to two groups: control (n = 9), with hemodynamic support aiming central venous pressure 8 to 12 mmHg, urinary output 0.5 mL/kg per hour, and mean arterial pressure greater than 65 mmHg; and SvO(2) (n = 9), with the goals above, plus SvO(2) greater than 65%. The interventions lasted 12 h, and lactated Ringer's and norepinephrine (both groups) and dobutamine (SvO(2) group) were administered. Inflammatory response was evaluated by plasma concentration of cytokines, neutrophil CD14 expression, oxidant generation, and apoptosis. Oxidative stress was evaluated by plasma and myocardial nitrate concentrations, myocardial and vascular NADP(H) oxidase activity, myocardial glutathione content, and nitrotyrosine expression. Mixed venous oxygen saturation-driven resuscitation was associated with improved systolic index, oxygen delivery, and diuresis. Sepsis induced in both groups a significant increase on IL-6 concentrations and plasma nitrate concentrations and a persistent decrease in neutrophil CD14 expression. Apoptosis rate and neutrophil oxidant generation were not different between groups. Treatment strategies did not significantly modify oxidative stress parameters. Thus, an approach aiming SvO(2) during sepsis improves hemodynamics, without any significant effect on inflammatory response and oxidative stress. The beneficial effects associated

  17. Arginine-rich cross-linking peptides with different SV40 nuclear localization signal content as vectors for intranuclear DNA delivery.

    Science.gov (United States)

    Bogacheva, Mariia; Egorova, Anna; Slita, Anna; Maretina, Marianna; Baranov, Vladislav; Kiselev, Anton

    2017-11-01

    The major barriers for intracellular DNA transportation by cationic polymers are their toxicity, poor endosomal escape and inefficient nuclear uptake. Therefore, we designed novel modular peptide-based carriers modified with SV40 nuclear localization signal (NLS). Core peptide consists of arginine, histidine and cysteine residues for DNA condensation, endosomal escape promotion and interpeptide cross-linking, respectively. We investigated three polyplexes with different NLS content (10 mol%, 50 mol% and 90 mol% of SV40 NLS) as vectors for intranuclear DNA delivery. All carriers tested were able to condense DNA, to protect it from DNAase I and were not toxic to the cells. We observed that cell cycle arrest by hydroxyurea did not affect transfection efficacy of NLS-modified carriers which we confirmed using quantitative confocal microscopy analysis. Overall, peptide carrier modified with 90 mol% of SV40 NLS provided efficient transfection and nuclear uptake in non-dividing cells. Thus, incorporation of NLS into arginine-rich cross-linking peptides is an adequate approach to the development of efficient intranuclear gene delivery vehicles. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Petition for Reconsideration from Biogenic CO2 Coalition to Gina McCarthy, U.S. EPA, for the Finding that Greenhouse Gas Emissions from Aircraft Cause or Contribute to Air Pollution that May Reasonably be Anticipated to Endanger Public Health and Welfare

    Science.gov (United States)

    This page contains a Petition for Reconsideration From Biogenic CO2 Coalition to Gina McCarthy, U.S. EPA, for the finding that greenhouse gas emissions from aircraft cause or contribute to air pollution that may reasonably be anticipated to endanger public

  19. Oncogenic transformation of rat lung epithelioid cells by SV40 DNA and restriction enzyme fragments

    International Nuclear Information System (INIS)

    Daya-Grosjean, L.; Lasne, C.; Nardeux, P.; Chouroulinkov, I.; Monier, R.

    1979-01-01

    Rat epithelioid lung cells were transformed with various preparations of SV40 DNA using the Ca 2+ -precipitation technique. The amount of SV40 genetic information integrated into transformed clones was evaluated by DNA-DNA renaturation kinetics. The growth properties on plastic and in soft-agar were examined, as well as the ability to induce tumors in syngeneic newborn animals or in adult nude mice. One particular transformed line, which had received the HpaII/BamHIA (59 per cent) fragment, was found to contain about 3 integrated copies of this fragment per cell and no significant amount of the HpaII/BamHIB (41 per cent fragment). This line which grew to high saturatio densities and efficiently formed clones in low serum on plastic, produced tumors in both syngeneic rats and nude mice. Thus the HpaII/BamHIA fragment, which mainly includes early viral information, was sufficient to impart these properties to rat epithelioid lung cells. (author)

  20. Role of Host-Driven Mutagenesis in Determining Genome Evolution of Sigma Virus (DMelSV; Rhabdoviridae) in Drosophila melanogaster.

    Science.gov (United States)

    Piontkivska, Helen; Matos, Luis F; Paul, Sinu; Scharfenberg, Brian; Farmerie, William G; Miyamoto, Michael M; Wayne, Marta L

    2016-10-05

    Sigma virus (DMelSV) is ubiquitous in natural populations of Drosophila melanogaster. Host-mediated, selective RNA editing of adenosines to inosines (ADAR) may contribute to control of viral infection by preventing transcripts from being transported into the cytoplasm or being translated accurately; or by increasing the viral genomic mutation rate. Previous PCR-based studies showed that ADAR mutations occur in DMelSV at low frequency. Here we use SOLiD TM deep sequencing of flies from a single host population from Athens, GA, USA to comprehensively evaluate patterns of sequence variation in DMelSV with respect to ADAR. GA dinucleotides, which are weak targets of ADAR, are strongly overrepresented in the positive strand of the virus, consistent with selection to generate ADAR resistance on this complement of the transient, double-stranded RNA intermediate in replication and transcription. Potential ADAR sites in a worldwide sample of viruses are more likely to be "resistant" if the sites do not vary among samples. Either variable sites are less constrained and hence are subject to weaker selection than conserved sites, or the variation is driven by ADAR. We also find evidence of mutations segregating within hosts, hereafter referred to as hypervariable sites. Some of these sites were variable only in one or two flies (i.e., rare); others were shared by four or even all five of the flies (i.e., common). Rare and common hypervariable sites were indistinguishable with respect to susceptibility to ADAR; however, polymorphism in rare sites were more likely to be consistent with the action of ADAR than in common ones, again suggesting that ADAR is deleterious to the virus. Thus, in DMelSV, host mutagenesis is constraining viral evolution both within and between hosts. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  1. Role of Host-Driven Mutagenesis in Determining Genome Evolution of Sigma Virus (DMelSV; Rhabdoviridae) in Drosophila melanogaster

    Science.gov (United States)

    Piontkivska, Helen; Matos, Luis F.; Paul, Sinu; Scharfenberg, Brian; Farmerie, William G.; Miyamoto, Michael M.; Wayne, Marta L.

    2016-01-01

    Abstract Sigma virus (DMelSV) is ubiquitous in natural populations of Drosophila melanogaster. Host-mediated, selective RNA editing of adenosines to inosines (ADAR) may contribute to control of viral infection by preventing transcripts from being transported into the cytoplasm or being translated accurately; or by increasing the viral genomic mutation rate. Previous PCR-based studies showed that ADAR mutations occur in DMelSV at low frequency. Here we use SOLiDTM deep sequencing of flies from a single host population from Athens, GA, USA to comprehensively evaluate patterns of sequence variation in DMelSV with respect to ADAR. GA dinucleotides, which are weak targets of ADAR, are strongly overrepresented in the positive strand of the virus, consistent with selection to generate ADAR resistance on this complement of the transient, double-stranded RNA intermediate in replication and transcription. Potential ADAR sites in a worldwide sample of viruses are more likely to be “resistant” if the sites do not vary among samples. Either variable sites are less constrained and hence are subject to weaker selection than conserved sites, or the variation is driven by ADAR. We also find evidence of mutations segregating within hosts, hereafter referred to as hypervariable sites. Some of these sites were variable only in one or two flies (i.e., rare); others were shared by four or even all five of the flies (i.e., common). Rare and common hypervariable sites were indistinguishable with respect to susceptibility to ADAR; however, polymorphism in rare sites were more likely to be consistent with the action of ADAR than in common ones, again suggesting that ADAR is deleterious to the virus. Thus, in DMelSV, host mutagenesis is constraining viral evolution both within and between hosts. PMID:27614234

  2. Comparative human cellular radiosensitivity: I. The effect of SV40 transformation and immortalisation on the gamma-irradiation survival of skin derived fibroblasts from normal individuals and from ataxia-telangiectasia patients and heterozygotes.

    Science.gov (United States)

    Arlett, C F; Green, M H; Priestley, A; Harcourt, S A; Mayne, L V

    1988-12-01

    We have compared cell killing following 60Co gamma irradiation in 22 primary human fibroblast strains, nine SV40-immortalized human fibroblast lines and seven SV40-transformed pre-crisis human fibroblast cultures. We have examined material from normal individuals, from ataxia-telangiectasia (A-T) patients and from A-T heterozygotes. We have confirmed the greater sensitivity of A-T derived cells to gamma radiation. The distinction between A-T and normal cells is maintained in cells immortalized by SV40 virus but the immortal cells are more gamma radiation resistant than the corresponding primary fibroblasts. Cells transformed by plasmids (pSV3gpt and pSV3neo) expressing SV40 T-antigen, both pre- and post-crisis, show this increased resistance, indicating that it is expression of SV40 T-antigen, rather than immortalization per se which is responsible for the change. We use D0, obtained from a straight line fit, and D, estimated from a multitarget curve, as parameters to compare radiosensitivity. We suggest that both have their advantages; D0 is perhaps more reproducible, but D is more realistic when comparing shouldered and non-shouldered data.

  3. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes.

    Science.gov (United States)

    Puthumana, Jayesh; Prabhakaran, Priyaja; Philip, Rosamma; Singh, I S Bright

    2015-12-01

    In an attempt of in vitro transformation, transfection mediated expression of Simian virus-40 (T) antigen (SV40-T) and transduction mediated expression of Adenovirus type 12 early region 1A (12S E1A) oncogene were performed in Penaeus monodon lymphoid cells. pSV3-neo vector encoding SV40-T oncogene and a recombinant baculovirus BacP2-12S E1A-GFP encoding 12S E1A oncogene under the control of hybrid promoters were used. Electroporation and lipofection mediated transformation of SV40-T in lymphoid cells confirmed the transgene expression by phenotypic variation and the expression of GFP in co-transfection experiment. The cells transfected by lipofection (≥ 5%) survived for 14 days with lower toxicity (30%), whilst on electroporation, most of the cells succumbed to death (60%) and survived cells lived up to 7 days. Transduction efficiency in primary lymphoid cells was more than 80% within 14 days of post-transduction, however, an incubation period of 7 days post-transduction was observed without detectable expression of 12S E1A. High level of oncogenic 12S E1A expression were observed after 14 day post-transduction and the proliferating cells survived for more than 90 days with GFP expression, however, without in vitro transformation and immortalization. The study put forth the requirement of transduction mediated 'specific' oncogene expression along with telomerase activation and epigenetic induction for the immortalization and establishment of shrimp cell line. Copyright © 2015. Published by Elsevier Ltd.

  4. Diagnostic accuracy of sub-mSv prospective ECG-triggering cardiac CT in young infant with complex congenital heart disease.

    Science.gov (United States)

    Gao, Wei; Zhong, Yu Min; Sun, Ai Min; Wang, Qian; Ouyang, Rong Zhen; Hu, Li Wei; Qiu, Han Sheng; Wang, Shi Yu; Li, Jian Ying

    2016-06-01

    To explore the clinical value and evaluate the diagnostic accuracy of sub-mSv low-dose prospective ECG-triggering cardiac CT (CCT) in young infants with complex congenital heart disease (CHD). A total of 102 consecutive infant patients (53 boys and 49 girls with mean age of 2.9 ± 2.4 m and weight less than 5 kg) with complex CHD were prospectively enrolled. Scans were performed on a 64-slice high definition CT scanner with low dose prospective ECG-triggering mode and reconstructed with 80 % adaptive statistical iterative reconstruction algorithm. All studies were performed during free breathing with sedation. The subjective image quality was evaluated by 5-point grading scale and interobserver variability was calculated. The objective image noise (standard deviation, SD) and contrast to noise ratio (CNR) was calculated. The effective radiation dose from the prospective ECG-triggering mode was recorded and compared with the virtual conventional retrospective ECG-gating mode. The detection rate for the origin of coronary artery was calculated. All patients also underwent echocardiography before CCT examination. 81 patients had surgery and their preoperative CCT and echocardiography findings were compared with the surgical results and sensitivity, specificity, positive and negative predictive values and accuracy were calculated for separate cardiovascular anomalies. Heart rates were 70-161 beats per minute (bpm) with mean value of 129.19 ± 14.52 bpm. The effective dose of 0.53 ± 0.15 mSv in the prospective ECG-triggering cardiac CT was lower than the calculated value in a conventional retrospective ECG-gating mode (2.00 ± 0.35 mSv) (p ECG-triggering CCT with sub-mSv effective dose provides excellent imaging quality and high diagnostic accuracy for young infants with complex CHD.

  5. Pleomorphic adenoma cells vary in their susceptibility to SV40 transformation depending on the initial karyotype.

    Science.gov (United States)

    Kazmierczak, B; Thode, B; Bartnitzke, S; Bullerdiek, J; Schloot, W

    1992-07-01

    Chromosomal aberrations involving 8q12 or 12q13-15 characterize two cytogenetic subgroups of salivary gland pleomorphic adenomas. As the tumors of the two groups differ in their clinical and histologic characteristics, we decided to determine their susceptibility to SV40 transformation. We transfected cell cultures from 13 adenomas with aberrations involving 8q12 and from seven adenomas with involvement of 12q13-15 using an SV40 plasmid coding for the early region of the viral genome. Whereas all cultures with aberrations of 12q13-15 showed transformed foci, only 4 of the 13 cultures with 8q12 abnormalities showed foci of transformed cells. We also observed a much higher immortalization rate in the first group (3/7 vs. 1/13). All successfully transformed tumor cell cultures showed a relatively stable karyotype in the pre-crisis stage and a high mitotic index, were T-antigen positive, and had an extended life span in vitro.

  6. Klar svækkelse af den faglige organisering de sidste ti år i Danmark

    DEFF Research Database (Denmark)

    Scheuer, Steen

    2006-01-01

    skyldes især at fagforbundene ikke har kunnet følge med den stigende arbejdsstyrke. Det påpeges desuden, at LO’s dominerende position på det organiserede arbejdsmarked bliver svækket, idet LO’s andel af samtlige fagligt organiserede er markant faldende, fra 73% i 1980 til 65% i dag, hvilket især skyldes...

  7. Estimation of Wave Conditions at Svåheia SSG Pilot Site

    DEFF Research Database (Denmark)

    Kofoed, Jens Peter; Margheritini, Lucia; Stratigaki, V.

    The purpose of the project described in the present report is to estimate the local wave conditions at the proposed location for a SSG pilot at the Svåheia site in the south western part of Norway. Focus is put on estimating the everyday conditions to enable an evaluation of the power production...... potential for the SSG pilot at the proposed location. The work in the project has been performed in three parts: 1. Establishing the offshore wave conditions and bathymetry of the area. 2. Transformation of offshore waves to near shore, through numerical wave modeling. 3. Evaluation of the transformed...... (local) wave conditions and its implications....

  8. Analogías en la enseñanza de la estequiometría: revisión de páginas web

    Directory of Open Access Journals (Sweden)

    Andrés Raviolo

    2014-12-01

    Full Text Available En este trabajo se presentan los resultados de una búsqueda sobre analogías para enseñar estequiometría, realizada en páginas web. Se analizan los resultados obtenidos a la luz de una serie de preguntas que plantea la investigación y se emiten sugerencias para la enseñanza. Se revisan las confusiones o concepciones alternativas que pueden promover y la utilización que se hace de las imágenes. Se concluye que un profesor encontrará en Internet muchos ejemplos de analogías, pero pocas secuencias adecuadas y escasas propuestas sistematizadas y fundamentadas a la luz del conocimiento de la Didáctica de las Ciencias. El trabajo con la analogía debería reforzar especialmente la comprensión profunda del significado de la ecuación química.

  9. Criação de páginas na internet, através do software, Front Page no processo ensino-aprendizagem como facilitador

    Directory of Open Access Journals (Sweden)

    Gildenir Carolino Santos

    2001-01-01

    Full Text Available Este artigo tem como objetivo, mostrar a capacidade de aprendizagem de duas pessoas, funcionárias da Biblioteca da Faculdade de Educação da UNICAMP, que possuem apenas conhecimentos em informática, e que através de um facilitador, aprenderão a utilizar o software Front Page (Microsoft para a elaboração de páginas na Internet, na linguagem HTML. This paper have how objective, to show the capacity of learning between two peoples, staff of Library of Faculty of Education/UNICAMP, that they can only knowledge in infomatics, and that através de um facilitador, will be to learn the to utilize the Front Page (Microsoft software for the elaborate the pages in the Internet, in HTML language.

  10. V. kongres světové literárněvědné bohemistiky

    Czech Academy of Sciences Publication Activity Database

    Holanová, Markéta; Jareš, Michal; Pišna, Jan; Segi, Stefan; Soukupová, K.

    2015-01-01

    Roč. 63, č. 5 (2015), s. 793-807 ISSN 0009-0468. [V. kongres světové literárněvědné bohemistiky: Válka a konflikt v české literatuře. Praha, 29.06.2015-04.07.2015] Institutional support: RVO:68378068 Keywords : Czech literature * war and conflict * congress * literary studies Subject RIV: AJ - Letters, Mass-media, Audiovision

  11. ginas de História da Enfermagem: o jubileu de ouro de uma obra (1951-2001 Páginas de História de la Enfermería: el jubileo de oro de una obra (1951-2001 History of Nursing Pages: the gold jubilee of a piece of work (1951-2001

    Directory of Open Access Journals (Sweden)

    Ana Paula Costa Alves

    2006-08-01

    Full Text Available O objeto deste estudo é a permanência do livro "Páginas de História da Enfermagem/História da Enfermagem" em referências acadêmicas em 2001, ano do cinqüentenário da primeira edição. OBJETIVO: Identificar a referência ao livro de Waleska Paixão nas fontes supracitadas. Metodologicamente, é um estudo histórico-social, de natureza quantitativa. Foi realizado levantamento bibliográfico nas dissertações e teses de três programas de pós-graduação, nos programas de disciplina de História da Enfermagem em três escolas públicas de graduação no município do Rio de Janeiro e artigos com descritores História da Enfermagem publicados na REBEn. RESULTADO: A análise revelou a citação do título em apenas um programa de disciplina de graduação. CONCLUSÃO: Apesar da importância do referido livro, devido ao seu pioneirismo no Brasil (1951, as citações desta obra estão desaparecendo nas referências pesquisadas, o que aponta para o surgimento de novos títulos e fontes para o estudo da História da Enfermagem.El objeto de este estudio es la permanencia del libro "Páginas de História de la Enfermería/História de la Enfermería" en referencias académicas en 2001, año del cincuentenario de la primera edición. El propósito es identificar la referencia al libro de Waleska Paixão en las fuentes antedichas. Metodologicamente es un estudio histórico-social, de naturaleza cuantitativa. Fué realizado levantamiento bibliográfico en las disertaciones y tesis de tres programas de posgrado, en los programas de la disciplina de Historia de la Enfermería en tres escuelas públicas de pregrado en el município del Rio de Janeiro y artículos con descritores Historia de la Enfermería publicados en la REBEn. RESULTADOS: El análisis reveló la citación del título sólo en un programa de disciplina de graduación. CONCLUSIÓN: A pesar de la importancia del libro ya mencionado, debido al su pionerismo en el Brasil (1951, las

  12. Identification of potential drug targets in Salmonella enterica sv. Typhimurium using metabolic modelling and experimental validation

    DEFF Research Database (Denmark)

    Hartman, Hassan B.; Fell, David A.; Rossell, Sergio

    2014-01-01

    Salmonella enterica sv. Typhimurium is an established model organism for Gram-negative, intracellular pathogens. Owing to the rapid spread of resistance to antibiotics among this group of pathogens, new approaches to identify suitable target proteins are required. Based on the genome sequence of ...

  13. Cerebral disorders of Chernobyl clean-up workers in remote period after irradiation in doses > 1 Sv

    International Nuclear Information System (INIS)

    Bomko, M.O.

    2004-01-01

    Neurologic, psychiatric and psychophysiologic (computed EEG) examinations were carried out in 45 men irradiated in doses > 1 Sv. Magnetic-resonance imaging (MRI) was carried out in 35 of them. Prevalence of negative psychopathologic symptoms, domination of low-voltage polymorphous types of EEG were the proves of organic nature of cerebral disorders in these patients. Pathologic changes in brain's structures were confirmed by MRI

  14. A Novel Experimental Set-Up for Improving the Sensitivity of SV Waves to Shallow Surface-Breaking Cracks

    Energy Technology Data Exchange (ETDEWEB)

    Pecorari, Claudio [Royal Inst. of Technology, Stockholm (Sweden). Dept. of Aeronautical and Vehicle Engineering

    2006-03-15

    Conventional inspection procedures to detect surface-breaking defects in train axels and thick pipes often employ 45-degree incidence shear vertical (SV) waves as probing tool. Recently obtained theoretical and experimental results indicate that this method is considerably less sensitivity to shallow surface-breaking defects, than the one in which the angle of incidence is selected to be close to the critical angle of the longitudinal wave. This project has confirmed this thesis by experimentally investigating the backscattering of SV waves by surface-breaking cracks as a function o t the angle of incidence. To this end, three cracks of depth approximately equal to 0.3 mm, 0.5 mm and 0.7 were introduced on the surface of steel samples with a thickness of 47 mm. These cracks were insonified with transducers operating at 2.25 MHz, 3.5 MHz, and 5 MHz, which correspond to wavelengths in steel of 1.38 mm, 0.88 mm, and 0.62 mm, respectively. The increase in sensitivity has been assessed in the order of 15 dB.

  15. Increased levels of SV2A botulinum neurotoxin receptor in clinical sensory disorders and functional effects of botulinum toxins A and E in cultured human sensory neurons

    Directory of Open Access Journals (Sweden)

    Yiangou Y

    2011-10-01

    Full Text Available Yiangos Yiangou1 Uma Anand1,2, William R. Otto2, Marco Sinisi3, Michael Fox3, Rolfe Birch3 Keith A. Foster4, Gaurav Mukerji1,5, Ayesha Akbar1,6, Sanjiv K. Agarwal5, Praveen Anand11Department of Clinical Neuroscience, Imperial College London, Hammersmith Hospital, London; 2Histopathology Laboratory, Cancer Research UK, London Research Institute, London; 3Peripheral Nerve Injury Unit, Royal National Orthopaedic Hospital, Stanmore; 4Syntaxin Ltd, Oxford; 5Department of Urology; 6Department of Gastroenterology, Imperial College London, Hammersmith Hospital, London, United Kingdom Background: There is increasing evidence that botulinum neurotoxin A may affect sensory nociceptor fibers, but the expression of its receptors in clinical pain states, and its effects in human sensory neurons, are largely unknown.Methods: We studied synaptic vesicle protein subtype SV2A, a receptor for botulinum neurotoxin A, by immunostaining in a range of clinical tissues, including human dorsal root ganglion sensory neurons, peripheral nerves, the urinary bladder, and the colon. We also determined the effects of botulinum neurotoxins A and E on localization of the capsaicin receptor, TRPV1, and functional sensitivity to capsaicin stimuli in cultured human dorsal root ganglion neurons.Results: Image analysis showed that SV2A immunoreactive nerve fibers were increased in injured nerves proximal to the injury (P = 0.002, and in painful neuromas (P = 0.0027; the ratio of percentage area SV2A to neurofilaments (a structural marker was increased proximal to injury (P = 0.0022 and in neuromas (P = 0.0001, indicating increased SV2A levels in injured nerve fibers. In the urinary bladder, SV2A nerve fibers were found in detrusor muscle and associated with blood vessels, with a significant increase in idiopathic detrusor overactivity (P = 0.002 and painful bladder syndrome (P = 0.0087. Colon biopsies showed numerous SV2A-positive nerve fibers, which were increased in quiescent

  16. Effects of Enrichment and Litter Parity on Reproductive Performance and Behavior in BALB/c and 129/Sv Mice.

    Science.gov (United States)

    Whitaker, Julia W; Moy, Sheryl S; Pritchett-Corning, Kathleen R; Fletcher, Craig A

    2016-01-01

    We examined the effect of adding species-appropriate environmental enrichment items to breeding cages of BALB/cAnNCrl and 129S2/SvPasCrl mice. The 3 enrichment conditions were: 1) cotton nesting material; 2) nesting material plus a paper shelter and rolled paper bedding; and 3) an igloo dome with an exercise wheel in addition to the shelter-group enrichments. We measured litter size, litter survival to weaning age, average pup weight at 21 d, and the interlitter interval to evaluate reproductive performance. A random subset of the first- or second-litter offspring from each enrichment condition and strain was assessed in multiple behavioral tests. Enrichment significantly affected anxiety-like behavior and sociability, with the direction of change dependent on strain and sex. Litter parity had greater effects on some reproductive parameters than did the enrichment condition, and this effect was not solely due to a difference between the first compared with subsequent litters. The significant effects of litter parity on the number of pups born and weaned, female pup weight, and interlitter interval were dependent on the enrichment condition in BALB/c but not 129/Sv mice. Offspring from the first or second litter were included in a generational component to investigate whether enrichment effects on reproduction persist in adult offspring after transfer to a different facility for breeding. Natal cage enrichment had no effect on any reproductive parameter in the transferred mice. Overall, additional enrichment beyond nesting material had a beneficial effect on the interlitter interval in BALB/c mice and on the number of pups weaned in 129/Sv mice.

  17. De la literatura misógina medieval a la igualdad de oportunidades entre hombres y mujeres. Descripción de una unidad didáctica para 3º de la ESO

    OpenAIRE

    Bataller Català, Alexandre

    2009-01-01

    Se explica y comenta los contenidos y secuenciación de una unidad didáctica que contiene como tema transversal la igualdad de oportunidades entre homes y mujeres, preparada para la asignatura de "Valencià. Llengua i literatura" para el curso de 3º de la ESO (que hemos publicado dentro de un proyecto editorial, Ediciones SM, 2007). Los contenidos literarios, tanto teóricos como prácticos, tratan tanto de la obra más celebrada de temática misógina del siglo XV, la novela en verso l'Spill, escri...

  18. STX140, but not paclitaxel, inhibits mammary tumour initiation and progression in C3(1/SV40 T/t-antigen transgenic mice.

    Directory of Open Access Journals (Sweden)

    Florence Meyer-Losic

    Full Text Available Despite paclitxael's clinical success, treating hormone-refractory breast cancer remains challenging. Paclitaxel has a poor pharmacological profile, characterized by a low therapeutic index (TIX caused by severe dose limiting toxicities, such as neutropenia and peripheral neuropathy. Consequently, new drugs are urgently required. STX140, a compound previously shown to have excellent efficacy against many tumors, is here compared to paclitaxel in three translational in vivo breast cancer models, a rat model of peripheral neuropathy, and through pharmacological testing. Three different in vivo mouse models of breast cancer were used; the metastatic 4T1 orthotopic model, the C3(1/SV40 T-Ag model, and the MDA-MB-231 xenograft model. To determine TIX and pharmacological profile of STX140, a comprehensive dosing regime was performed in mice bearing MDA-MD-231 xenografts. Finally, peripheral neuropathy was examined using a rat plantar thermal hyperalgesia model. In the 4T1 metastatic model, STX140 and paclitaxel significantly inhibited primary tumor growth and lung metastases. All C3(1/SV40 T-Ag mice in the control and paclitaxel treated groups developed palpable mammary cancer. STX140 blocked 47% of tumors developing and significantly inhibited growth of tumors that did develop. STX140 treatment caused a significant (P<0.001 survival advantage for animals in early and late intervention groups. Conversely, in C3(1/SV40 T-Ag mice, paclitaxel failed to inhibit tumor growth and did not increase survival time. Furthermore, paclitaxel, but not STX140, induced significant peripheral neuropathy and neutropenia. These results show that STX140 has a greater anti-cancer efficacy, TIX, and reduced neurotoxicity compared to paclitaxel in C3(1/SV40 T-Ag mice and therefore may be of significant benefit to patients with breast cancer.

  19. New candidate tumor-suppressor gene KLF6 and its splice variant KLF6 SV2 counterbalancing expression in primary hepatocarcinoma.

    Science.gov (United States)

    Zhenzhen, Zhou; De'an, Tian; Limin, Xia; Wei, Yan; Min, Luo

    2012-01-01

    This study aimed to detect the expression of newly discovered zinc finger transcriptional factor KLF6 and its splice variant KLF6 SV2 in primary hepatocarcinoma (PHC) tissues and hepatoma cell strains, and to evaluate their clinicopathologic relationship with PHC. Wild-type KLF6 and KLF6 SV2 mRNA expression was determined by RTPCR in 27 cases of PHC tissues and cell strains of HepG2, SMMC7721 and LO2. Western blotting and immunohistochemical staining were adopted to detect KLF6 protein expression. Positive area ratio of wild-type KLF6 protein expression and its relationship with clinicopathological parameters of PHC was analyzed. Wild-type KLF6 expression in PHC tissues was lower than that in paracancerous tissues. In contrast, KLF6 SV2 mRNA expression was higher in PHC tissues and hepatoma cell strains (p<0.05). Positive area ratio of wild-type KLF6 protein expression was positively correlated with cellular differentiation degree of PHC (p<0.01), but negatively correlated not only with liver cirrhosis, tumor size and extrahepatic metastases (p<0.01), but also with portal vein thrombus and the number of lymph nodes with metastasis (p<0.05). Wild-type KLF6 deletion and inactivation was involved in the growth, cell differentiation and other physiological processes of PHC. The upregulation of KLF6 splice variant might counterbalance the wildtype KLF6 and contribute to the occurrence and development of PHC.

  20. Defective repair of UV-damaged DNA in human tumor and SV40-transformed human cells but not in adenovirus-transformed human cells

    International Nuclear Information System (INIS)

    Rainbow, A.J.

    1989-01-01

    The DNA repair capacities of five human tumor cell lines, one SV40-transformed human cell line and one adenovirus-transformed human cell line were compared with that of normal human fibroblasts using a sensitive host cell reactivation (HCR) technique. Unirradiated and UV-irradiated suspensions of adenovirus type 2 (Ad 2) were assayed for their ability to form viral structural antigens (Vag) in the various cell types using immunofluorescent staining. The survival of Vag formation for UV-irradiated Ad 2 was significantly reduced in all the human tumor cell lines and the SV40-transformed human line compared to the normal human fibroblasts, but was apparently normal in the adenovirus-transformed human cells. D 0 values for the UV survival of Ad 2 Vag synthesis in the tumor and virally transformed lines expressed as a percentage of that obtained on normal fibroblast strains were used as a measure of DNA repair capacity. Percent HCR values ranged from 26 to 53% in the tumor cells. These results indicate a deficiency in the repair of UV-induced DNA damage associated with human tumorigenesis and the transformation of human cells by SV40 but not the transformation of human cells by adenovirus. (author)

  1. GINA WEB: UNA HERRAMIENTA VIRTUAL PARA LA ENSEÑANZA-APRENDIZAJE DE LA GENÉTICA EN EL GRADO OCTAVO DE LA INSTITUCIÓN EDUCATIVA MARÍA CRISTINA ARANGO DE LA CIUDAD DE NEIVA, HUILA

    Directory of Open Access Journals (Sweden)

    Dora Magaly García Ibarra

    2015-12-01

    Full Text Available En esta ponencia presentamos una experiencia de aula desarrollada al interior  del espacio académico de  Práctica Pedagógica II del Programa de  Licenciatura en Ciencias Naturales: Física, Química y Biología de la Universidad Surcolombiana (Neiva, Huila  desarrollada con un grupo de 30 estudiantes de grado octavo de la Institución Educativa María Cristina Arango de la ciudad de Neiva durante el 2015-I. El problema de investigación está enmarcado en el campo de la Didáctica de las Ciencias y particularmente de las TIC aplicadas a la educación, ya que éstas son  un conjunto de medios y herramientas tecnológicas de la informática y la comunicación  que se pueden utilizar en pro del aprendizaje en este caso de la Genética. La investigación se propone desde un enfoque cualitativo - descriptivo empleando análisis de contenido, acción participante, el cuestionario y la página web. En éste sentido, consideramos que el diseño, aplicación y sistematización de la Página web es indispensable para contemplar las temáticas, finalidades y estrategias de enseñanza de la genética para contribuir aprendizajes más enriquecidos en las ciencias naturales.

  2. The ICRP Proposed Maximum Public Dose Constraints of o.3 mSv/y: a Major Issue for the Nuclear Industry

    International Nuclear Information System (INIS)

    Saint-Pierre, S.; Coates, R.

    2004-01-01

    The International Commission on Radiological Protection (ICRP) is currently developing a new set of Recommendations on Radiological Protection. A value of 0.3mSv/y for the maximum public dose constraint has been discussed by ICRP. This value represents a major concern for the nuclear industry at large. The primary issue arises from the lack of any new scientific evidence on public health effects from ionising radiation to support, in practice, the proposed reduction by about a factor of 3 (from 1 to 0.3 mSv/y) of the upper bound value for public dose impact from a nuclear activity or site. Such a change would create a de facto limit on public exposure from specific sources at a dose level of about one tenth of average natural background and an even smaller fraction of the typical range of background exposures and exposures from medical sources. This cannot be justified on public health grounds. The WNA supports ICRP's renewed intention, as expressed at the NEA-ICRP Stakeholder Forum in Lanzarote (April 2003), to retain the concept of a public dose limit at 1 mSv/y. We strongly believe that the current system comprising of the dose limit and the ALARA Principle provides the necessary flexibility and tools for regulators to address all situations in all countries. The WNA consider that the question of setting an upper bound dose constraint (below 1 mSv/y) at the country/site specific level is best left for discussion and agreement between the local stakeholders rather than at an international level. When considering the potential practical implications of a maximum dose constraint, it is important to look beyond the very low off-site dose impacts (on the public) resulting from annual routine radioactive discharges of nuclear industrial sites. There are many off-site and on-site practical situations, related to public exposures (both workers and the public) and worker classification as well as activities such transportation, decommissioning and site remediation, for

  3. The ICRP Proposed Maximum Public Dose Constraints of o.3 mSv/y: a Major Issue for the Nuclear Industry

    Energy Technology Data Exchange (ETDEWEB)

    Saint-Pierre, S.; Coates, R.

    2004-07-01

    The International Commission on Radiological Protection (ICRP) is currently developing a new set of Recommendations on Radiological Protection. A value of 0.3mSv/y for the maximum public dose constraint has been discussed by ICRP. This value represents a major concern for the nuclear industry at large. The primary issue arises from the lack of any new scientific evidence on public health effects from ionising radiation to support, in practice, the proposed reduction by about a factor of 3 (from 1 to 0.3 mSv/y) of the upper bound value for public dose impact from a nuclear activity or site. Such a change would create a de facto limit on public exposure from specific sources at a dose level of about one tenth of average natural background and an even smaller fraction of the typical range of background exposures and exposures from medical sources. This cannot be justified on public health grounds. The WNA supports ICRP's renewed intention, as expressed at the NEA-ICRP Stakeholder Forum in Lanzarote (April 2003), to retain the concept of a public dose limit at 1 mSv/y. We strongly believe that the current system comprising of the dose limit and the ALARA Principle provides the necessary flexibility and tools for regulators to address all situations in all countries. The WNA consider that the question of setting an upper bound dose constraint (below 1 mSv/y) at the country/site specific level is best left for discussion and agreement between the local stakeholders rather than at an international level. When considering the potential practical implications of a maximum dose constraint, it is important to look beyond the very low off-site dose impacts (on the public) resulting from annual routine radioactive discharges of nuclear industrial sites. There are many off-site and on-site practical situations, related to public exposures (both workers and the public) and worker classification as well as activities such transportation, decommissioning and site remediation

  4. La comunicación corporativa a través de las páginas Web: el caso de las universidades españolas

    Directory of Open Access Journals (Sweden)

    Alejandro Tapia Frade

    2011-11-01

    Full Text Available La comunicación corporativa supone un activo de suma importancia para cualquier organización. También para las universidades, que se encuentran en un proceso de convergencia que exige la generación de un discurso corporativo que se adapte al nuevo entorno competidor. Así, este trabajo analiza la comunicación corporativa de las universidades españolas a través de sus portales Web mediante la técnica de análisis de contenido con perspectiva cuantitativa. Los resultados ponen de manifiesto que aunque la presencia de una sección específica destinada a ésta en las páginas analizadas es mayoritaria, la gestión de contenidos de la misma no puede calificarse de buena, ya que no se incluye toda la información que debería, y todavía más, no se aprovecha la flexibilidad del soporte para generar discurso corporativo multimedia.

  5. Amplification of oncogenes and integrated SV40 sequences in mammalian cells by the decay of incorporated iodine-125

    International Nuclear Information System (INIS)

    Ehrfeld, A.; Planas-Bohne, F.; Luecke-Huhle, C.

    1986-01-01

    Iodine-125, in the form of 5-[ 125 I]iododeoxyuridine (I-UdR), was incorporated into the DNA of SV40 transformed Chinese hamster embryo cells. Disintegration of the 125 I led to increased cell killing with increasing dose as measured by the colony-forming ability of single cells. The D37 (the dose at which 37% of the cells survive) amounts to 95 decays per cell, corresponding to 0.66 Gy. Variations in the copy number of specific DNA sequences was measured by using dispersed cell blotting with sensitive DNA hybridizations. A 13-fold amplification of the viral DNA sequences (SV40) and a twofold amplification of two cellular oncogenes of the ras-family (Ki-ras and Ha-ras) were found. Other cellular genes, like the alpha-actin gene, were not amplified, and no variation in gene copy number was detected after incubation of cells with cold I-UdR. We suggest the observed gene amplifications are induced by the densely ionizing radiation emitted by the decay of the incorporated 125 I atoms

  6. Efficient production and secretion of bovine beta-lactoglobulin by Lactobacillus casei.

    Science.gov (United States)

    Hazebrouck, Stéphane; Pothelune, Laetitia; Azevedo, Vasco; Corthier, Gérard; Wal, Jean-Michel; Langella, Philippe

    2007-04-06

    Lactic acid bacteria (LAB) are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine beta-lactoglobulin (BLG), a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23. Using a nisin-inducible plasmid system, we first showed that L. casei BL23 strain could efficiently secrete a reporter protein, the staphylococcal nuclease (Nuc), with the lactococcal signal peptide SPUsp45 fused to its N-terminus. The fusion of SPUsp45 failed to drive BLG secretion but led to a 10-fold increase of intracellular BLG production. Secretion was significantly improved when the synthetic propeptide LEISSTCDA (hereafter called LEISS) was added to the N-terminus of the mature moiety of BLG. Secretion rate of LEISS-BLG was 6-fold higher than that of BLG alone while intracellular production reached then about 1 mg/L of culture. The highest yield of secretion was obtained by using Nuc as carrier protein. Insertion of Nuc between LEISS and BLG resulted in a 20-fold increase in BLG secretion, up to 27 microg/L of culture. Furthermore, the lactococcal nisRK regulatory genes were integrated into the BL23 chromosome. The nisRK insertion allowed a decrease of BLG synthesis in uninduced cultures while BLG production increased by 50% after nisin induction. Moreover, modification of the induction protocol led to increase the proportion of soluble BLG to around 74% of the total BLG production. BLG production and secretion in L. casei were significantly improved by fusions to a propeptide enhancer and a carrier protein. The resulting recombinant strains will be further tested for their ability to modulate the immune response

  7. Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

    Directory of Open Access Journals (Sweden)

    Wal Jean-Michel

    2007-04-01

    Full Text Available Abstract Background Lactic acid bacteria (LAB are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine β-lactoglobulin (BLG, a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23. Results Using a nisin-inducible plasmid system, we first showed that L. casei BL23 strain could efficiently secrete a reporter protein, the staphylococcal nuclease (Nuc, with the lactococcal signal peptide SPUsp45 fused to its N-terminus. The fusion of SPUsp45 failed to drive BLG secretion but led to a 10-fold increase of intracellular BLG production. Secretion was significantly improved when the synthetic propeptide LEISSTCDA (hereafter called LEISS was added to the N-terminus of the mature moiety of BLG. Secretion rate of LEISS-BLG was 6-fold higher than that of BLG alone while intracellular production reached then about 1 mg/L of culture. The highest yield of secretion was obtained by using Nuc as carrier protein. Insertion of Nuc between LEISS and BLG resulted in a 20-fold increase in BLG secretion, up to 27 μg/L of culture. Furthermore, the lactococcal nisRK regulatory genes were integrated into the BL23 chromosome. The nisRK insertion allowed a decrease of BLG synthesis in uninduced cultures while BLG production increased by 50% after nisin induction. Moreover, modification of the induction protocol led to increase the proportion of soluble BLG to around 74% of the total BLG production. Conclusion BLG production and secretion in L. casei were significantly improved by fusions to a propeptide enhancer and a carrier protein. The resulting recombinant strains will be further tested

  8. Predialing the number of cinegraphic frames enables an effective patient dose due to coronary angiography of 0.8 mSv

    International Nuclear Information System (INIS)

    Kuon, E.; Schmitt, M.; Dorn, C.; Pfahlberg, A.; Gefeller, O.; Dahm, J.B.

    2003-01-01

    Purpose: To investigate the effect of a new device for predialing the number of cinegraphic frame before each coronary angiography, with the objective of reducing the patient dose area product (DAP) from coronary angiography, which typically requires 1000 to 2350 cinegraphic frames. That DAP is high and stated to be between 15.6 to 106.3 Gy x cm 2 . Applying the accepted DAP-to-ED conversion factors, for the thoracic region of approximately 0.20 mSv/Gy x cm 2 , this corresponds to a mean effective dose (ED) in the range of 3.1 to 21.3 mSv. Material and Methods: For patients undergoing elective coronary angiography, we compared various parameters of radiation exposure obtained with judicious radiation reducing standard techniques (n=106) and with an additional new rotary switch for predialing the number of cinegraphic frames (n=106). Results: The patient radiation dose was significantly lower with the new device, with the mean DAP reduced to 5.5 from 9.1 Gy x cm 2 . The corresponding reducation of the mean DAP for left ventriculography and coronary angiography was 1.3 from 1.7, and 4.2 from 7.4 Gy x cm 2 , respectively. The number of cinegraphic frames was 98 vs. 184, whereas the number of cinegraphic runs and the fluoroscopy time were comparable. Conclusion: Predialing the cinegraphic frame number before each cinegraphic run enables a reduction of the patients effective dose from coronary angiography to 0.8 mSv, i.e. to 57% of the baseline value and far below typically reported values. (orig.) [de

  9. As percepções acerca do Nazismo presentes nas páginas do jornal A Offensiva

    Directory of Open Access Journals (Sweden)

    Murilo Antonio Paschoaleto

    2011-05-01

    Full Text Available

    A Ação Integralista Brasileira, movimento de extrema direita brasileiro, fundado, oficialmente, por Plínio Salgado em Outubro de 1932, contou com uma extensa rede de periódicos. Tais periódicos foram de suma importância para o desenvolvimento do movimento, pois, suas páginas, além de serem o canal mais eficaz para a difusão da doutrina apregoada pela AIB, funcionavam, concomitantemente, como meio de propaganda tanto ideológica quanto para a arregimentação de novos militantes. A cúpula integralista, apesar de admitir sua admiração aos demais movimentos fascistas existentes no mundo, a todo o momento recusava as afirmações de que o Integralismo seria um fascismo. Acreditamos ser frutífera uma análise de como os próprios integralistas, no calor daquele contexto, se viam e se posicionavam diante dos movimentos fascistas. Para tanto, levando-se em consideração a própria natureza dos jornais e tendo-se em vista a importância central desempenhada por estes dentro do movimento, acreditamos ser pertinente uma análise de como a imprensa integralista retratava o universo fascista.  Assim, neste trabalho, ao procurarmos colher indícios de como o principal periódico do movimento, o jornal A Offensiva, se posicionava diante da Alemanha e o Nazismo, notamos que o jornal, naquele contexto e no calor dos acontecimentos, se posicionava à favor dos avanços e conquistas nazistas.

  10. SvSXP: a Strongylus vulgaris antigen with potential for prepatent diagnosis.

    Science.gov (United States)

    Andersen, Ulla V; Howe, Daniel K; Dangoudoubiyam, Sriveny; Toft, Nils; Reinemeyer, Craig R; Lyons, Eugene T; Olsen, Susanne N; Monrad, Jesper; Nejsum, Peter; Nielsen, Martin K

    2013-04-04

    Strongyle parasites are ubiquitous in grazing horses. Strongylus vulgaris, the most pathogenic of the large strongyles, is known for its extensive migration in the mesenteric arterial system. The lifecycle of S. vulgaris is characterised by a long prepatent period where the migrating larvae are virtually undetectable as there currently is no test available for diagnosing prepatent S. vulgaris infection. Presence of S. vulgaris larvae in the arterial system causes endarteritis and thrombosis with a risk of non-strangulating intestinal infarctions. Emergence of anthelmintic resistance among cyathostomins has led to recommendations of reduced treatment intensity by targeting horses that exceed a predetermined strongyle faecal egg count threshold. One study suggests an apparent increase in prevalence of S. vulgaris on farms where reduced anthelmintic treatment intensity has been implemented. These issues highlight the need for an accurate and reliable assay for diagnosing prepatent S. vulgaris infection. Immunoscreening of a larval S. vulgaris cDNA library using hyperimmune serum raised against S. vulgaris excretory/secretory antigens was performed to identify potential diagnostic antigens. Immunoreactive clones were sequenced, one potential antigen was characterised, expressed as a recombinant protein, initially evaluated by western blot (WB) analysis, the diagnostic potential of the IgG subclasses was evaluated by ELISA, and the diagnostic accuracy evaluated using serum from 102 horses with known S. vulgaris infection status. The clone expressing the potential antigen encoded a S. vulgaris SXP/RAL2 homologue. The recombinant protein, rSvSXP, was shown to be a potential diagnostic antigen by WB analysis, and a target of serum IgGa, IgG(T) and total IgG in naturally infected horses, with IgG(T) antibodies being the most reliable indicator of S. vulgaris infection in horses. Evaluation of diagnostic accuracy of the ELISA resulted in a sensitivity of 73.3%, a specificity

  11. La imagen electrónica de la energía. Estudio estético y funcional de las páginas webs de las compañías del sector energético

    OpenAIRE

    Álvarez Ruiz, Antón; Reyes Moreno, María Isabel

    2011-01-01

    Este artículo analiza las páginas webs de las principales compañías operantes en el sector de la energía (electricidad y gas) de los países más desarrollados. La situación actual del mercado es muy significativa. Por primera vez en la historia, las administraciones centrales de estos países han consentido liberalizar el mercado energético. Y, aunque este proceso avanza con gran lentitud, comienzan a notarse cambios que anticipan un proceso de concentración, a través de compras, fusiones y ...

  12. Transcriptional repression is epigenetically marked by H3K9 methylation during SV40 replication

    OpenAIRE

    Kallestad, Les; Christensen, Kendra; Woods, Emily; Milavetz, Barry

    2014-01-01

    Background We have recently shown that T-antigen binding to Site I results in the replication-dependent introduction of H3K9me1 into SV40 chromatin late in infection. Since H3K9me2 and H3K9me3 are also present late in infection, we determined whether their presence was also related to the status of ongoing transcription and replication. Transcription was either inhibited with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidizole (DRB) or stimulated with sodium butyrate and the effects on histone m...

  13. Dedicated sub 0.1 mSv 3DCT using MBIR in children with suspected craniosynostosis: quality assessment

    Energy Technology Data Exchange (ETDEWEB)

    Ernst, Caroline W.; Hulstaert, Tine L.; Belsack, Dries; Buls, Nico; Gompel, Gert van; Nieboer, Koenraad H.; Verhelle, Filip; Maeseneer, Michel de; Mey, Johan de [Universitair Ziekenhuis Brussel, Department of Radiology, Brussels (Belgium); Buyl, Ronald [Vrije Universiteit Brussel, Department of Biomedical Statistics and Informatics, Brussels (Belgium)

    2016-03-15

    To retrospectively compare image quality of a lowered dose CT protocol to a standard CT protocol in children with suspicion of craniosynostosis. Forty-eight patients (age 0- 35 months), who presented with a cranial deformity underwent cranial 3D CT to assess sutural patency: between 2009 - 2010, 24 patients were imaged with a standard protocol (CTDIvol 32.18 mGy), from 2011-2012, 24 underwent a low dose protocol (0.94 mGy) combined with iterative reconstruction. Image quality was evaluated by both expert reading and objective analysis. Differences were assessed by independent t-test and Mann-Whitney U test, interreader agreement by Cohen's Kappa test. Effective dose of the low dose protocol was 0.08 mSv, corresponding to a reduction of 97 %. Image quality was similar in both groups in terms of overall diagnostic acceptability, objective noise measurements, subjective cranial bone edge sharpness and presence of artefacts. For objective sharpness of cranial bone-brain interface and subjective perception of noise, the images of the low dose protocol were superior. For all evaluated structures, interreader agreement was moderate to almost perfect. In the diagnosis of craniosynostosis in children with cranial deformities, a dedicated sub 0.1 mSv cranial 3DCT protocol can be used without loss in image quality. (orig.)

  14. Ambtscriminaliteit aangegeven? : Een onderzoek naar het opvolgen van en kennis over de wettelijke verplichting tot aangifte van artikel 162 Sv misdrijven

    NARCIS (Netherlands)

    Vries Robbé, E. de; Cornelissens, A.; Ferwerda, H.

    2008-01-01

    Dit is een onderzoek naar de werking van de aangifteplicht bij ambtscriminaliteit (art 162 Sv). Het gaat dan bijvoorbeeld om het aannemen van steekpenningen en misdrijven waarbij ambtenaren een bijzondere ambtsplicht schenden. De aangifteplicht raakt de klokkenluidersregeling en maakt onderdeel uit

  15. Immune cells in Chernobyl recovery operation workers exposed over 500 mSv

    International Nuclear Information System (INIS)

    Bazyka, D.; Byelyaeva, N.; Chumak, A.; Golyarnik, N.; Maznichenko, O.; Kovalenko, Ju.

    2004-01-01

    Immune response parameters were studied in Chernobyl radiation emergency workers exposed to radiation over 500 mSv during 1986. Initial response stage to the radiation exposure was characterised by immunological deficiency with T-cell subsets changes. In the reconstitution period inhibition of immune function was associated with lymphocyte subset changes such as decreased CD3 + and CD4 + cell counts and increased number of somatic mutations in TCR-locus. Late period after the acute radiation exposure in Chernobyl radiation emergency workers is characterized by decreased CD8 + suppressor cell function that could lead to poor proliferation control. Subset analysis of CD34 + cells showed in ARS survivors counts significantly higher than in control especially for the most primitive progenitors with CD34 + CD90 + CD45 -/l ow and CD34 + CD45 - CD38 - phenotypes. (author)

  16. EVIDENCIAÇÃO DE CAPITAL INTELECTUAL NAS PÁGINAS WEB DE PREFEITURAS DO RIO GRANDE DO SUL

    Directory of Open Access Journals (Sweden)

    Clari Schuh

    2013-12-01

    Full Text Available Esta pesquisa objetivou analisar as informações sobre capital intelectual evidenciadas nas páginas WEB dos municípios do Estado do Rio Grande do Sul, mediante pesquisa documental, partindo-se de um instrumento de pesquisa composto de itens referentes aos componentes de capital intelectual – capital humano, capital estrutural, capital de munícipes e transparência –, assim como os seus respectivos subitens. Os dados foram objeto de análise descritiva e testes estatísticos. Como resultados do estudo, podem-se mencionar: (a o teste Tukey de comparação múltipla de proporções revelou não haver diferença significativa na proporção de evidenciação de capital intelectual entre os municípios de Porto Alegre, Bagé, Canoas, Caxias do Sul, Santa Cruz do Sul, São Leopoldo e Sapucaia do Sul; (b comparando-se a proporção de evidenciação por item, constatou-se que a transparência apresentou uma proporção de 65,90%, seguida do capital dos munícipes (43,10%, capital estrutural (36,80% e capital humano (26,70%. Considera-se, finalmente, que há espaço para uma maior evidenciação de capital intelectual pelas prefeituras, pois, além do compromisso com a transparência e a comunicação com os cidadãos, a evidenciação de informações poderá agregar valor aos municípios.

  17. A página virtual do movimento dos trabalhadores rurais sem terra (MST) como instrumento de contrainformação na luta político-ideológica pela reforma agrária

    OpenAIRE

    Engelmann, Solange Inês

    2013-01-01

    O presente trabalho teve como objetivo a análise da página nacional de internet do Movimento dos Trabalhadores Rurais Sem Terra (MST), criada em 1997. Nosso propósito foi compreender o processo de construção do portal e qual a importância desse novo aparato de comunicação na divulgação das demandas do MST e no debate da Reforma Agrária no Brasil, para o diálogo com a classe trabalhadora urbana e sua base social. Para tanto, inicialmente apresentamos uma discussão sobre o novo processo de t...

  18. Sequence-selective topoisomerase II inhibition by anthracycline derivatives in SV40 DNA: Relationship with DNA binding affinity and cytotoxicity

    International Nuclear Information System (INIS)

    Capranico, G.; Kohn, K.W.; Pommier, Y.; Zunino, F.

    1990-01-01

    Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and β-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding affinity. Cleavage of 32 P-end-labeled DNA fragments was visualized by autoradiography of agarose and polyacrylamide gels. Structure-activity relationships indicated that alterations in the chromophore structure greatly affected drug action on topoisomerase II. In particular, removal of substituents on position 4 of the D ring resulted in more active inducers of cleavage with lower DNA binding affinity. The stereochemistry between the sugar and the chromophore was also essential for activity. All the active anthracyclines induced a single region of prominent cleavage in the entire SV40 DNA, which resulted from a cluster of sites between nucleotides 4237 and 4294. DNA cleavage intensity patterns exhibited differences among analogues and were also dependent upon drug concentration. Intensity at a given site dependent on both stimulatory and suppressive effects depending upon drug concentration and DNA sequence. A good correlation was found between cytotoxicity and intensity of topoisomerase II mediated DNA breakage

  19. School and writing. A school page in the daily newspaper Escuela y escritura. Una página escolar en la prensa diaria

    Directory of Open Access Journals (Sweden)

    Teresa GONZÁLEZ PÉREZ

    2013-07-01

    Full Text Available The innovative and vanguard experience known as «La Isla de los Niños» that was accomplished in the Canary Islands by the teacher Ricardo García Luis, represents the reality of writing done in the schools. His ability to prepare students for participation in a form of media communication led to the fusion of educational, cultural and social elements that facilitated a dialog between the students and the world. The purpose was not to prepare a student publication for the educational center, but rather to publish in the daily newspaper. The children expressed themselves freely on diverse topics and themes at a time in which the Franco dictatorship represented definite censorship. Initially the work came out of the professional work of the aforementioned teacher, in a school in the town of La Zarza, in the municipality of Arico (south of the island of Tenerife, that discovered the ability to narrate, describe, interpret and retell the activities of his students that he felt worthy of notice. He broke with the mold and all educational stereotypes and opened new horizons for his students when he edited the material written in his school and published in the newspaper El Día a weekly paper from Santa Cruz de Tenerife.La experiencia innovadora y vanguardista que realizó en Canarias el maestro Ricardo García Luis, conocida como «La Isla de los Niños», representa la proyección de la escritura desde la escuela. Su conciencia y disposición para habilitar a los escolares para su intervención y proyección en un medio de comunicación condujo a la conjugación educativa, cultural y social, a través de la cual los alumnos dialogaban con el mundo. Porque no se trataba de elaborar una página escolar para el centro educativo, sino para insertarla en la prensa diaria. De acuerdo con los objetivos de su creador, los niños se expresaban libremente sobre diversos aspectos y temas en una etapa aún amordazada por la dictadura franquista. En un

  20. Effect of substrate composition on atomic layer deposition using self-assembled monolayers as blocking layers

    International Nuclear Information System (INIS)

    Zhang, Wenyu; Engstrom, James R.

    2016-01-01

    The authors have examined the effect of two molecules that form self-assembled monolayers (SAMs) on the subsequent growth of TaN x by atomic layer deposition (ALD) on two substrate surfaces, SiO 2 and Cu. The SAMs that the authors have investigated include two vapor phase deposited, fluorinated alkyl silanes: Cl 3 Si(CH 2 ) 2 (CF 2 ) 5 CF 3 (FOTS) and (C 2 H 5 O) 3 Si(CH 2 ) 2 (CF 2 ) 7 CF 3 (HDFTEOS). Both the SAMs themselves and the TaN x thin films, grown using Ta[N(CH 3 ) 2 ] 5 and NH 3 , were analyzed ex situ using contact angle, spectroscopic ellipsometry, x-ray photoelectron spectroscopy (XPS), and low energy ion-scattering spectroscopy (LEISS). First, the authors find that both SAMs on SiO 2 are nominally stable at T s  ∼ 300 °C, the substrate temperature used for ALD, while on Cu, the authors find that HDFTEOS thermally desorbs, while FOTS is retained on the surface. The latter result reflects the difference in the head groups of these two molecules. The authors find that both SAMs strongly attenuate the ALD growth of TaN x on SiO 2 , by about a factor of 10, while on Cu, the SAMs have no effect on ALD growth. Results from LEISS and XPS are decisive in determining the nature of the mechanism of growth of TaN x on all surfaces. Growth on SiO 2 is 2D and approximately layer-by-layer, while on the surfaces terminated by the SAMs, it nucleates at defect sites, is islanded, and is 3D. In the latter case, our results support growth of the TaN x thin film over the SAM, with a considerable delay in formation of a continuous thin film. Growth on Cu, with or without the SAMs, is also 3D and islanded, and there is also a delay in the formation of a continuous thin film as compared to growth on SiO 2 . These results highlight the power of coupling measurements from both LEISS and XPS in examinations of ultrathin films formed by ALD

  1. Numerical Study on Dynamic Response of a Horizontal Layered-Structure Rock Slope under a Normally Incident Sv Wave

    Directory of Open Access Journals (Sweden)

    Zhifa Zhan

    2017-07-01

    Full Text Available Several post-earthquake investigations have indicated that the slope structure plays a leading role in the stability of rock slopes under dynamic loads. In this paper, the dynamic response of a horizontal layered-structure rock slope under harmonic Sv wave is studied by making use of the Fast Lagrangian Analysis of Continua method (FLAC. The suitability of FLAC for studying wave transmission across rock joints is validated through comparison with analytical solutions. After parametric studies on Sv wave transmission across the horizontal layered-structure rock slope, it is found that the acceleration amplification coefficient η, which is defined as the ratio of the acceleration at the monitoring point to the value at the toe, wavily increases with an increase of the height along the slope surface. Meanwhile, the fluctuation weakens with normalized joint stiffness K increasing and enhances with normalized joint spacing ξ increasing. The acceleration amplification coefficient of the slope crest ηcrest does not monotonously increase with the increase of ξ, but decreases with the increase of K. Additionally, ηcrest is more sensitive to ξ compared to K. From the contour figures, it can also be found that the contour figures of η take on rhythm, and the effects of ξ on the acceleration amplification coefficient are more obvious compared to the effects on K.

  2. Reseña de: Aguiar Andrade, Amélia y Millán da Costa, Adelaide (Eds. La ville Médiévale en Débat. Instituto de Estudos Medievais, Lisboa, 2013, 206 páginas (Estudos, 8, (Idiomas Francés, Español e Inglés. ISBN: 978-989-97066-9-9.

    Directory of Open Access Journals (Sweden)

    Ana María Rivera Medina

    2017-05-01

    Full Text Available Aguiar Andrade, Amélia y Millán da Costa, Adelaide (Eds. La ville Médiévale en Débat. Instituto de Estudos Medievais, Lisboa, 2013, 206 páginas (Estudos, 8, (Idiomas Francés, Español e Inglés. isbn: 978-989-97066-9-9.

  3. Homologous SV40 RNA trans-splicing: Special case or prime example of viral RNA trans-splicing?

    Directory of Open Access Journals (Sweden)

    Sushmita Poddar

    2014-06-01

    Full Text Available To date the Simian Virus 40 (SV40 is the only proven example of a virus that recruits the mechanism of RNA trans-splicing to diversify its sequences and gene products. Thereby, two identical viral transcripts are efficiently joined by homologous trans-splicing triggering the formation of a highly transforming 100 kDa super T antigen. Sequences of other viruses including HIV-1 and the human adenovirus type 5 were reported to be involved in heterologous trans-splicing towards cellular or viral sequences but the meaning of these events remains unclear. We computationally and experimentally investigated molecular features associated with viral RNA trans-splicing and identified a common pattern: Viral RNA trans-splicing occurs between strong cryptic or regular viral splice sites and strong regular or cryptic splice sites of the trans-splice partner sequences. The majority of these splice sites are supported by exonic splice enhancers. Splice sites that could compete with the trans-splicing sites for cis-splice reactions are weaker or inexistent. Finally, all but one of the trans-splice reactions seem to be facilitated by one or more complementary binding domains of 11 to 16 nucleotides in length which, however occur with a statistical probability close to one for the given length of the involved sequences. The chimeric RNAs generated via heterologous viral RNA trans-splicing either did not lead to fusion proteins or led to proteins of unknown function. Our data suggest that distinct viral RNAs are highly susceptible to trans-splicing and that heterologous viral trans-splicing, unlike homologous SV40 trans-splicing, represents a chance event.

  4. Dissociation of DNA damage and mitochondrial injury caused by hydrogen peroxide in SV-40 transformed lung epithelial cells

    Directory of Open Access Journals (Sweden)

    Adcock Ian M

    2002-11-01

    Full Text Available Abstract Background Since lung epithelial cells are constantly being exposed to reactive oxygen intermediates (ROIs, the alveolar surface is a major site of oxidative stress, and each cell type may respond differently to oxidative stress. We compared the extent of oxidative DNA damage with that of mitochondrial injury in lung epithelial cells at the single cell level. Result DNA damage and mitochondrial injury were measured after oxidative stress in the SV-40 transformed lung epithelial cell line challenged with hydrogen peroxide (H2O2. Single cell analysis of DNA damage was determined by assessing the number of 8-oxo-2-deoxyguanosine (8-oxo-dG positive cells, a marker of DNA modification, and the length of a comet tail. Mitochondrial membrane potential, ΔΨm, was determined using JC-1. A 1 h pulse of H2O2 induced small amounts of apoptosis (3%. 8-oxo-dG-positive cells and the length of the comet tail increased within 1 h of exposure to H2O2. The number of cells with reduced ΔΨm increased after the addition of H2O2 in a concentration-dependent manner. In spite of a continual loss of ΔΨm, DNA fragmentation was reduced 2 h after exposure to H2O2. Conclusion The data suggest that SV-40 transformed lung epithelial cells are resistant to oxidative stress, showing that DNA damage can be dissociated from mitochondrial injury.

  5. Hypothalamic neurosecretory and circadian vasopressinergic neuronal systems in the blind cone-rod homeobox knock out mouse (Crx(-/-) ) and the 129sv wild type mouse

    DEFF Research Database (Denmark)

    Rovsing, Louise; Rath, Martin Fredensborg; Møller, Morten

    2013-01-01

    circadian AVP-rhythm. We have in this study of the brown 129sv mouse and the visual blind cone-rod homeobox gene knock out mouse (Crx(-/-) ) with degeneration of the retinal rods and cones, but a preserved non-image forming optic system, studied the temporal Avp-expression in both the neurosecretory...

  6. Musikterapi med børn med svær autisme - en litteraturgennemgang

    Directory of Open Access Journals (Sweden)

    Ulla Holck

    2003-03-01

    Full Text Available Faglitteratur om musikterapi med børn med autisme er omfattende og indeholder såvel kvalitative case-beskrivelser som kvantitative forskningsundersøgelser. I artiklen gennemgås faglitteraturen systematisk med henblik på at specifi cere musikterapiens effekt og virkemåder i forhold til denne målgruppe. Vægten ligger på børn med svær autisme, men litteratur om voksne højtfungerende personer med autisme inddrages også. Forskningslitteraturen viser, at det især er inden for områderne koncentration, visuel opmærksomhed, respons og initiativ, samt brug af stemme og tur-tagning, at musikterapi har en effekt. Case-litteraturen begrunder denne effekt med musikkens evne til at være redundant, anvendelse af imitation og responsfremmende teknikker (overraskelse etc., fælles opbyggede samspilsformer, samt det temporale-interaktive element i improvisatorisk musikterapi. Ud fra en interaktionsteoretisk indfaldsvinkel sammenkobles effekten endvidere med, at den musikalske interaktion hjælper musikterapeuten til at fastholde et dynamisk udtryk, hvilket er afgørende i forhold til en klientgruppe, der ofte giver ´flad´ eller stærkt afvigende feedback.

  7. Cryo-electron microscopy of vitrified SV40 minichromosomes: the liquid drop model.

    Science.gov (United States)

    Dubochet, J; Adrian, M; Schultz, P; Oudet, P

    1986-03-01

    The structure of SV40 minichromosomes has been studied by cryo-electron microscopy of vitrified thin layers of solution. In high-salt buffer (130 mM NaCl), freshly prepared minichromosomes are condensed into globules 30 nm or more in diameter. On the micrograph, they appear to be formed by the close packing of 10 nm granules which give rise to a 10 nm reflection in the optical diffractogram. The globules can adopt many different conformations. At high concentration, they fuse into a homogeneous 'sea' of closely packed 10 nm granules. In low-salt buffer (less than 10 mM NaCl), the globules open, first into 10 nm filaments, and then into nucleosome-strings. The 'liquid drop' model is proposed to explain the condensed structure of the minichromosome in high-salt buffer: nucleosomes stack specifically on top of one another, thus forming the 10 nm filaments. 10 nm filaments in turn, tend to aggregate laterally. Optimizing both these interactions results in the condensation of 10 nm filaments or portions thereof into a structure similar to that of a liquid. Some implications of this model for the structure of cellular chromatin are discussed.

  8. New components for the neutron spectrometer SV5c at the 10H channel of the research reactor FRJ2

    International Nuclear Information System (INIS)

    Stockmeyer, R.

    1991-02-01

    The following new components have been installed at the neutron time-of-flight spectrometer SV5c: 1. Monochromator with devices for tilting and rotating 10 crystal. The fine-adjustment of the crystal orientation can be done with a computer program which maximizes the neutron intensity at the sample position. 2. 128 x 512 multi-channel time-of-flight electronic 3. Computerized equipment for measuring thermal properties of a sample (adsorption isotherm, sample transmission) 4. Data aquisition, data handling and experiment control software coded in ASYST. (orig.)

  9. Structure and evaluation of antibacterial and antitubercular properties of new basic and heterocyclic 3-formylrifamycin SV derivatives obtained via 'click chemistry' approach.

    Science.gov (United States)

    Pyta, Krystian; Klich, Katarzyna; Domagalska, Joanna; Przybylski, Piotr

    2014-09-12

    Thirty four novel derivatives of 3-formylrifamycin SV were synthesized via reductive alkylation and copper(I)-catalysed azide-alkyne cycloaddition. According to the obtained results, 'click chemistry' can be successfully applied for modification of structurally complex antibiotics such as rifamycins, with the formation of desired 1,2,3-triazole products. However, when azide-alkyne cycloaddition on 3-formylrifamycin SV derivatives demanded higher amount of catalyst, lower temperature and longer reaction time because of the high volatility of substrates, an unexpected intramolecular condensation with the formation of 3,4-dihydrobenzo[g]quinazoline heterocyclic system took place. Structures of new derivatives in solution were determined using one- and two-dimensional NMR methods and FT-IR spectroscopy. Computational DFT and PM6 methods were employed to correlate their conformation and acid-base properties to biological activity and establish SAR of the novel compounds. Microbiological, physico-chemical (logP, solubility) and structural studies of newly synthesised rifamycins indicated that for the presence of relatively high antibacterial (MIC ~0.01 nmol/mL) and antitubercular (MIC ~0.006 nmol/mL) activities, a rigid and basic substituent at C(3) arm, containing a protonated nitrogen atom "open" toward intermolecular interactions, is required. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  10. Biomolecules from olive pruning waste in Sierra Mágina - Engaging the energy transition by multi-actor and multidisciplinary analyses.

    Science.gov (United States)

    Cohen, Marianne; Lepesant, Gilles; Lamari, Farida; Bilodeau, Clelia; Benyei, Petra; Angles, Stéphane; Bouillon, Julien; Bourrand, Kevin; Landoulsi, Ramla; Jaboeuf, Delphine; Alonso-Roldán, Maria; Espadas-Tormo, Isidro; Belandria, Veronica; Silar, Philippe; Dicko, Moussa

    2018-06-15

    The price volatility of fossil resources, the uncertainty of their long-term availability and the environmental, climatic and societal problems posed by their operation lead to the need of an energy transition enabling the development and utilization of other alternative and sustainable resources. Acknowledging that indirect land-use change can increase greenhouse gas emission, the European Union (EU) has reshaped its biofuel policy. It has set criteria for sustainability to ensure that the use of biofuels guarantees real carbon savings and protects biodiversity. From a sustainability perspective, biofuels and bioliquids offer indeed both advantages (e.g., more secure energy supply, emission reductions, reduced air pollution and production of high added-value molecules) as well as risks (monocultures, reduced biodiversity and even higher emissions through land use change). Approaching economic, environmental and social sustainability at the local level and in an integrated way should help to maximize benefits and minimize risks. This approach has been adopted and is described in the present work that combines chemical, biological, social and territorial studies on the management of pruning waste residues from olive trees in the Sierra Mágina in Spain. The biological and social analyses helped to orientate the research towards an attractive chemical process based on extraction and pyrolysis, in which high added value molecules are recovered and in which the residual biochar may be used as pathogen-free fertilizer. In this region where farmers face declining economic margins, the new intended method may both solve greenhouse gas emission problems and provide farmers with additional revenues and convenient fertilizers. Further research with a larger partnership will consolidate the results and tackle issues such as the logistics. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Matrix metalloproteinase-9 (MMP9) is involved in the TNF-α-induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells.

    Science.gov (United States)

    Weiler, Julian; Mohr, Marieke; Zänker, Kurt S; Dittmar, Thomas

    2018-04-10

    In addition to physiological events such as fertilisation, placentation, osteoclastogenesis, or tissue regeneration/wound healing, cell fusion is involved in pathophysiological conditions such as cancer. Cell fusion, which applies to both the proteins and conditions that induce the merging of two or more cells, is not a fully understood process. Inflammation/pro-inflammatory cytokines might be a positive trigger for cell fusion. Using a Cre-LoxP-based cell fusion assay we demonstrated that the fusion between human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells was induced by the pro-inflammatory cytokine tumour necrosis factor-α (TNF-α). The gene expression profile of the cells in the presence of TNF-α and under normoxic and hypoxic conditions was analysed by cDNA microarray analysis. cDNA microarray data were verified by qPCR, PCR, Western blot and zymography. Quantification of cell fusion events was determined by flow cytometry. Proteins of interest were either blocked or knocked-down using a specific inhibitor, siRNA or a blocking antibody. The data showed an up-regulation of various genes, including claudin-1 (CLDN1), ICAM1, CCL2 and MMP9 in M13SV1-Cre and/or MDA-MB-435-pFDR1 cells. Inhibition of these proteins using a blocking ICAM1 antibody, CLDN1 siRNA or an MMP9 inhibitor showed that only the blockage of MMP9 was correlated with a decreased fusion rate of the cells. Likewise, the tetracycline-based antibiotic minocycline, which exhibits anti-inflammatory properties, was also effective in both inhibiting the TNF-α-induced MMP9 expression in M13SV1-Cre cells and blocking the TNF-α-induced fusion frequency of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells. The matrix metalloproteinase-9 (MMP9) is most likely involved in the TNF-α-mediated fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells. Likewise, our data indicate that the tetracycline

  12. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Diego Iturriaga Barco

    2009-11-01

    Full Text Available Anguita, Julio, Corazón rojo. La vida después de un infarto. Madrid, La esfera de los libros, 2005, 251 pp.Páginas 169-171Diego Iturriaga BarcoAttinà, Fulvio; Rossi, Rosa (ed., European Neighbourhood Policy: Political, Economic and Social Issues. Catania, The Jean Monnet Centre "Euro-Med", 166 pp.Páginas 171-172Alejandro Román AntequeraCastells, Manuel; Serra, Narcís (eds., Guerra y Paz en el Siglo XXI. Una Perspectiva Europea. Barcelona, Tusquets Editores, 2003, 208 pp.Páginas 172-175Jesús Fernández GarcíaChomsky, Noam, Hegemonía o Supervivencia. La estrategia imperialista de Estados Unidos. Barcelona, Ediciones B, 2004, 401 pp.Páginas 175-177Jesús Fernández GarcíaClarke, Richard C., Contra todos los enemigos. Madrid, Taurus, 2004, 381 pp.Páginas 177-179Inmaculada Ramírez PérezDunn, Ross E., The New World History: A Teacher's Companion. New York, Bedford/St. Martins, 2000, 596 pp.Páginas 179-183Andre Gunder FrankFausto, Boris; Devoto, Fernando J., Brasil e Argentina: Um ensaio de história comparada (1850-2002. São Paulo, Editora 34, 2004, 574 pp.Páginas 183-184Paulo Roberto de AlmeidaFûret, François, El pasado de una ilusión. Ensayo sobre la idea comunista en el siglo XX. Madrid, Fondo de Cultura Económica, 1995, 583 pp.Páginas 184-185Alfonso Galindo LucasGill, Stephen; Mittelman, James, Innovation and transformation in International Studies. Cambridge & New York, Cambridge University Press, 1997, 294 pp.Páginas 185-189Andre Gunder FrankMussolini, Romano, Il duce mio padre. Milano, Rizzoli, 2004, 170 pp.Páginas 189-195Jan NelisNavajas Zubeldia, Carlos (ed., Actas del IV Simposio de Historia Actual. 2 Vols., Logroño, 2004, 1.090 pp.Páginas 195-197Alejandro Román AntequeraRichardson, Philip, Economic Change in China, c.1800-1950. Cambridge, Cambridge University Press, 1999, 137 pp.Páginas 197-198Andre Gunder FrankSartori, Giovanni; Mazzoleni, Gianni, La tierra explota. Superpoblación y desarrollo. Madrid, Taurus

  13. p53 levels, cell cycle kinetics and radiosensitivity in two SV40 transformed Wi38VA13 fibroblast strains

    International Nuclear Information System (INIS)

    Werner, F.; Zoelzer, F.; Streffer, C.

    2001-01-01

    Background: The tumor suppressor protein p53 which can mediate an ionizing radiation-induced G 1 arrest in mammalian cells, forms complexes with SV40 large T antigen (l-T-Ag). We have analyzed the p53 levels, the capability to undergo a G 1 arrest and the radiosensitivity of two SV40 transformed fibroblast strains differing in their large T antigen expression. Material and Methods: One of the two strains (VA13F) is the commercially available form of Wi38VA13, the other (VA13E) arose spontaneously from the original one in our laboratory. Their p53 levels were measured by means of flow cytometry (FCM) and Western blot (WB) with two p53 antibodies (Ab-3, clone PAb240; Ab-6, clone DO-1; both Oncogene Science). Cell cycle distributions were determined flow cytometrically after BrdU labeling at regular time intervals after exposure to 250 kV X-rays. Radiosensitivity was assessed in a clonogenicity assay. Results: The p53 levels of the two strains corresponded to their large T antigen expression, presumably due to complex formation between the two proteins. The strain with a high p53 level did not show a G 1 arrest and had a relatively high radiosensitivity, whereas the strain with a low p53 level showed a significant G 1 arrest and a lower radiosensitivity. Conclusion: These results suggest that 1. complex formation between the large T antigen and p53 reduces the latter's functionality; 2. in these two strains the G 1 arrest is one of the factors determining radiosensitivity. (orig.) [de

  14. Maestro di Pico i iluminacije u inkunabuli De Civitate Dei (Nicolas Jenson, Venecija, 1475. u samostanu Sv. Duje u Kraju na Pašmanu

    Directory of Open Access Journals (Sweden)

    Bojan Goja

    2014-12-01

    Full Text Available U franjevačkom samostanu Sv. Duje u Kraju na otoku Pašmanu čuva se inkunabula „O državi Božjoj“ (De Civitate Dei Sv. Aurelija Augustina koju je 1475. godine u Veneciji tiskao Nicolas Jenson. Inkunabula je na f. 17 (kako je naknadno označena paginacija grafitnom olovkom, tj. stranici incipita – liber primus kao i na više drugih stranica ukrašena lijepim višebojnim iluminacijama renesansnih stilskih karakteristika izrađenih temperom, zlatnim listićima, zlatnim prahom, sepijom, tintom i akvarelom. Iluminacije f. 17 sastoje se od dva fitomorfna inicijala, dekorativnog okvira i slobodnih figurativnih prizora tj. minijatura, dok su pojedine druge stranice ukrašene fitomorfnim i brojnim inicijala tipa littera notabilior visine dva retka izrađenima u crvenoj ili plavoj boji. Dekorativni ornamentirani okvir zaključen trolistima ispunjava gornju i lijevu marginu početne stranice. Osnovu dekoracije čini traka sastavljena od pet tankih linija koja se spiralno povija i tvori kružnice koje su ispunjene cvjetovima, listovima i bobicama u plavoj i zelenoj boji i boji ciklame te stiliziranim zlatnim cvjetovima čička (lat. Arctium lappa; pojedini autori nazivaju ove ukrasne motive bottoni dorati ili gold dots. U gornjem lijevom uglu okvira naslikan je češljugar. Na mjestu gdje započinje tekst lijevog stupca naslikan je unutar pravokutnog polja na zlatnoj pozadini inicijal „I“ sastavljen od fitomorfnih motiva plave, zelene i boje ciklame s osjenčanjima u različitim nijansama. Unutar dekorativnog okvira, na sredini visine inicijala, nalazi se medaljon unutar kojeg je naslikano poprsje Sv. Aurelija Augustina. Dekorativni ornamentirani okvir koji ispunjava gornju marginu ukrašen je na isti način kao i onaj uz desnu ali skromnije. Ime tiskara dodatno je naznačeno tako da je sa gornje i donje strane okvira ukrašeno sa po jednim stiliziranim zlatnim cvijetom čička sa gornje i češljugovine (lat. Dipsacus fullonum sa donje strane

  15. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Carlos L. Yordan

    2009-12-01

    Full Text Available Bacevich, Andrew J., The New American Militarism: How Americans are seduced by war. New York, Oxford University Press, 2005, 288 pp.Páginas 201-203Carlos L. YordanGirona Albuixech, Albert; Santacreu Soler, José Miguel (coords., La crisis de la Segunda República. Valencia, Prensa Valenciana, 2006, 143 pp.Páginas 203-206Antonio J. Piqueres DíezGonzález del Miño, Paloma, Las relaciones entre España y Marruecos. Perspectivas para el Siglo XXI. Madrid, Los Libros de la Catarata, 2005, 225 pp.Páginas 206-208Marcela Iglesias OnofrioGuereña, Jean–Louis, La prostitución en la España contemporánea. Marcial Pons, Madrid, 2003, 471 pp.Páginas 208-210Alejandro Román AntequeraHamilton, Clive, El fetiche del crecimiento. Pamplona, Laetoli, 2006, 254 pp.Páginas 210-214José Antonio Ruiz GilKlare, Michael T., Sangre y petróleo. Peligros y consecuencias de la dependencia del crudo. Barcelona, Urano, 2006, 396 pp.Páginas 214-216David Molina RabadánLarraza Micheltorena, María del Mar (dir., De leal a disidente: Pamplona, 1936-1977. Pamplona, Ediciones Eunate, 2006, 330 pp.Páginas 216-218Joaquín Piñeiro BlancaLedesma, José Luis, Los días de llamas de la revolución. Violencia y política en la retaguardia republicana de Zaragoza durante la guerra civil. Zaragoza, Institución Fernando el Católico, 2004, 364 pp.Páginas 218-219José Luis Gutiérrez MolinaMartín de la Guardia, Ricardo; Pérez Sánchez, Guillermo Á.; Szilágyi, István, La Batalla de Budapest. Historia de la insurrección húngara de 1956. Madrid, Actas, 2006, 295 pp.Páginas 219-221Virginia Martín JiménezMedina, Francisco, 23-F. La verdad. Barcelona, Plaza y Janés, 2006, 448 pp.Páginas 221-225Alfonso Pinilla GarcíaMoreno Pestaña, José Luis, Convirtiéndose en Foucault: sociogénesis de un filósofo. Mataró, Montesinos, 2006, 208 pp.Páginas 225-228Alejandro Estrella González“Política de alianza y estrategias unitarias en la historia del PCE”. Papeles de la

  16. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Héctor Vega Deloya

    2010-01-01

    Full Text Available Arizpe, Lourdes, Culturas en movimiento: Interactividad cultural y procesos globales. México, Cámara de los diputados LIX Legislatura, UNAM, CRIM, Editorial Miguel Ángel Porrúa, 2006, 368 pp.Páginas 203-206Héctor Vega DeloyaCárcel Ortí, Vicente, La Iglesia y la transición española. Valencia, Edicep C. B., 2003, 342 pp.Páginas 206-207Gonzalo Ruiz BidónChaput, Marie-Claude (éd., De l´anarquisme aux courants alternatifs (XIX-XXIe siècles. Régards 9, París, Publidix, Université de Paris X-Nanterre, 2006, 460 pp.Páginas 208-210Francisco de Paula Villatoro SánchezChuse, Loren, Mujer y flamenco. Sevilla, Signatura Ediciones, 2007, 228 pp.Páginas 210-212Gema León RavinaCores Trasmonte, Baldomero; Luis Porteiro Garea, A Coruña, TresCtres, 2005, 260 pp.Páginas 212-213Israel Sanmartín BarrosDíez Torre, Alejandro R., Trabajan para la eternidad. Colectividades de Trabajo y Ayuda Mutua durante la Guerra Civil en Aragón. Madrid, La Malatesta Editorial, Prensas Universitarias de Zaragoza, 2009, 540 pp.Páginas 213-216José Luis Gutiérrez MolinaGonin, Jean Marc; Guez, Olivier, La caída del Muro de Berlín, Madrid, Alianza Editorial, 2009, 367 pp.Páginas 216-217Mª del Rocío Piñeiro ÁlvarezHardy, Jane, Poland´s new capitalism, London, Pluto Press, 2009, 258 pp.Páginas 217-218Daniel Alcalde GüelfoKaplan, Robert D., Por tierra, mar y aire. Las huellas globales del ejército americano. Barcelona, Ediciones B, 2008, 542 pp.Páginas 218-220David Molina RabadánMartínez, Jesús Manuel, Salvador Allende. Oviedo, Ediciones Nobel, 2009, 402 pp.Páginas 220-223Juan Gustavo Núñez OlguínTravaglio, Marco, La scomparsa dei fatti. Milano, Collana Pamphlet, Il Sagiatore, 2006, 316 pp.Páginas 223-225Flavia PascarielloZertal, Idith; Eldar, Akiva, Lords of the Land: The War for Israeli´s Settlements in The Occupied Territories, 1967-2007. Nueva York, Nation Books, 2009, 531 pp.Páginas 225-227Javier Lión Bustillo

  17. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    José Antonio Ruiz Gil

    2009-12-01

    Full Text Available Beck, Ulrich; Grande, Edgar, La Europa Cosmopolita. Sociedad y Política en la Segunda Modernidad. Barcelona, Paidós, 2006, 392 pp.Páginas 187-189José Antonio Ruiz GilCastells, Manuel (ed., La sociedad red: una visión global. Madrid, Alianza Editorial, 2006, 558 pp.Páginas 189-191David Molina RabadánCuenca Toribio, José Manuel, Nacionalismo, Franquismo y Nacionalcatolicismo. Madrid, Editorial Actas, 2008, 220 pp.Páginas 191-193Ricardo Martín de la GuardiaDiamond, Jared, Colapso, Por qué unas sociedades perduran y otras desaparecen. Madrid, Debate, 2006, 854 pp.Páginas 193-195Daniel Alcalde GüelfoFumaroli, Marc, El Estado cultural (ensayo sobre una religión moderna. Barcelona, Acantilado, 2007, 147 pp.Páginas 195-197José Antonio Ruiz GilGarí, Manuel; Pastor, Jaime y Romero, Miguel (eds., 1968. El mundo pudo cambiar de base. Madrid, Los Libros de la Catarata, 2008, 365 pp.Páginas 197-199Ana Domínguez RamaMann, James, Los Vulcanos. El gabinete de guerra de Bush: Cheney, Powell, Rumsfeld, Rice, Wolfowitz, Armitage. Granada, Almed, 2007, 498 pp.Páginas 199-201David Molina RabadánMartín Muñoz, Gema, Iraq. Un fracaso de Occidente (1920-2003. Barcelona, Tusquets, 2003, 323 pp.Páginas 201-202José María Torrente GisbertMuñoz Aunión, Antonio, La política común europea de derecho de asilo. Valencia, Tirant Lo Blanc. 2007, 268 pp.Páginas 202-205José Joaquín Fernández AllesRey Tristán, Eduardo (dir., Memorias de la violencia en Uruguay y Argentina. Golpes, dictaduras, exilios (1973-2006. Santiago de Compostela, Universidad de Santiago de Compostela-CIEAM, 2007, 382 pp.Páginas 205-206Silvina JensenShlaim, Avi, El Muro de Hierro. Israel y el mundo árabe. Granada, Almed, 2003, 778 pp.Páginas 207-208José María Torrente GisbertWallerstein, Immanuel, Utopística o las opciones históricas del siglo XXI. Madrid, Siglo XXI Editores, 1998, 91 pp.Páginas 208-209Manuel Pérez Salinas

  18. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Daniel Alcalde Güelfo

    2009-12-01

    Full Text Available Achcar, Gilbert, El choque de barbaries, terrorismo y desorden mundial. Barcelona, Icaria-Antrazyt, 2007, 197 pp.Páginas 213-214Daniel Alcalde GüelfoDel Arco Blanco, Miguel Ángel, Hambre de Siglos. Mundo rural y apoyos sociales del franquismo en Andalucía Oriental (1936-1951. Granada, Comares Historia, 2007, 513 pp.Páginas 214-217Mª Candelaria Fuentes NavarroBen-Ghiat, Ruth; Fuller, Mia (eds., Italian Colonialism. New York, Palgrave MacMillan, 2005, 266 pp.Páginas 217-219Karl J. TrybusBlack, Edwin, IBM y el Holocausto: La alianza estratégica entre la Alemania nazi y la más poderosa corporación norteamericana. Buenos Aires, Atlántida, 2001, 508 pp.Páginas 219-220Flavia PascarielloCasassas, Jordi (coord., La construcción del presente. El mundo desde 1848 hasta nuestros días. Barcelona, Ariel, 2005, 696 pp.Páginas 220-223Alfonso Pinilla GarcíaFerguson, Niall, La guerra del mundo. Los conflictos del siglo XX y el declive de Occidente (1904-1953. Barcelona, Debate, 2007, 888 pp.Páginas 223-225David Molina RabadánGentile, Emilio, Fascismo di pietra. Roma-Bari, Laterza, 2007, 284 pp.Páginas 225-227Jan NelisKelly, Eamonn, La década decisiva. Tres escenarios para el futuro del mundo. Barcelona, Granica, 2007, 318 pp.Páginas 227-228David Molina RabadánLuzzatto, Sergio, The Body of Il Duce: Mussolini’s Corpse and the Fortunes of Italy. New York, Metropolitan Books, 2005, 258pp.Páginas 228-230Karl J. TrybusSánchez Blanco, Ángel, Organización Intermunicipal. Madrid, Iustel, Biblioteca de Derecho Municipal, 2006, 258 pp.Páginas 230-232José Joaquín Fernández AllesSantacreu Soler, José Miguel; Aura Murcia, Federico y Millán Llin, Vicente, El municipi del liberalisme: Sant Vicent del Raspeig 1806-1848. Simat de la Valldigna, Edicions La Xarxa, 2004, 156 pp.Páginas 232-235Antonio J. Piqueres Díez

  19. Role of unsaturated derivatives of spermidine as substrates for spermine synthase and in supporting growth of SV-3T3 cells.

    OpenAIRE

    Pegg, A E; Nagarajan, S; Naficy, S; Ganem, B

    1991-01-01

    Synthetic unsaturated analogues of the natural polyamine were examined as possible substrates for spermine synthase and as replacements for spermidine in supporting the growth of SV-3T3 cells. It was found that N-(3-aminopropyl)-1,4-diamino-cis-but-2-ene [the cis isomer of the alkene analogue of spermidine] was a good substrate for spermine synthase, but that the trans isomer [N-(3-aminopropyl)-1,4-diamino-trans-but-2-ene] and the alkene analogue [N-(3-aminopropyl)-1,4-diaminobut-2-yne] were ...

  20. Equilibrium Investment Strategy for DC Pension Plan with Inflation and Stochastic Income under Heston’s SV Model

    Directory of Open Access Journals (Sweden)

    Jingyun Sun

    2016-01-01

    Full Text Available We consider a portfolio selection problem for a defined contribution (DC pension plan under the mean-variance criteria. We take into account the inflation risk and assume that the salary income process of the pension plan member is stochastic. Furthermore, the financial market consists of a risk-free asset, an inflation-linked bond, and a risky asset with Heston’s stochastic volatility (SV. Under the framework of game theory, we derive two extended Hamilton-Jacobi-Bellman (HJB equations systems and give the corresponding verification theorems in both the periods of accumulation and distribution of the DC pension plan. The explicit expressions of the equilibrium investment strategies, corresponding equilibrium value functions, and the efficient frontiers are also obtained. Finally, some numerical simulations and sensitivity analysis are presented to verify our theoretical results.

  1. Growth and hydrogenation of ultra-thin Mg films on Mo(111)

    DEFF Research Database (Denmark)

    Ostenfeld, Christopher Worsøe; Davies, Jonathan Conrad; Vegge, Tejs

    2005-01-01

    . Hydrogen cannot be adsorbed on magnesium films under UHV conditions. However, when evaporating Mg in a hydrogen background, a hydrogen overlayer is seen to adsorb at the Mg surface, due to the catalytic interaction with the Mo(1 1 1) substrate and subsequent spill-over. We show that two monolayers of Mg......The growth and hydrogenation of ultra-thin magnesium overlayers have been investigated on a Mo(1 1 1) single crystal substrate. For increasing magnesium coverages we observe intermediate stages in the TPD and LEISS profiles, which illustrate the transition from one monolayer to multilayer growth...

  2. Reseñas de libros

    Directory of Open Access Journals (Sweden)

    Alfonso Galindo Lucas

    2009-03-01

    Full Text Available Ali, Tariq, Bush en Babilonia. La recolonización de Irak. Madrid, Alianza Editorial, 2004, 286 pp.Páginas 183-185Alfonso Galindo LucasÁlvarez Espinosa, Daniel, Cristianos y marxistas contra Franco. Cádiz, Servicio de Publicaciones de la Universidad de Cádiz, 2003, 454 pp.Páginas 185-187Gonzalo Ruiz BidónBonhoeffer, Dietrich, Resistencia y sumisión. Cartas y apuntes desde el cautiverio. Salamanca, Sígueme, 2003, 300 pp.Páginas 187-188Daniel Francisco Álvarez EspinosaCabrera, Miguel Ángel, Historia, Lenguaje y Teoría de la Sociedad. Madrid, Cátedra Universitat de Valencia, 2001, 188 pp.Páginas 188-191Alejandro Estrella GonzálezCobo Romero, Francisco, De campesinos a electores. Modernización agraria en Andalucía, politización campesina y derechización de los pequeños propietarios y arrendatarios. El caso de la provincia de Jaén, 1931-1936. Madrid, B. Nueva, 2003, 358 pp.Páginas 191-193David Molina RabadánColomer Viadel, Antonio, Crisis y Reformas en Iberoamérica ¿... y la Revolución? Valencia, Editorial Nomos, 2002, 231 pp.Páginas 193-196Jesús Fernández GarcíaCué, Carlos E., ¡Pásalo!: Los cuatro días de marzo que cambiaron el país. Barcelona, Ediciones Península Atalaya, 2004, 143 pp.Páginas 196-198Inmaculada Ramírez PérezCuenca Toribio, José Manuel, Catolicismo social y político en la España contemporánea (1870-2000. Madrid, Unión Editorial, 2003, 451 pp.Páginas 198-200Fernando López MoraKagan, Robert, Poder y debilidad. Europa y EEUU en el nuevo orden mundial. Madrid, Taurus, 2003, 165 pp.Páginas 201-202David Molina RabadánNapoleoni, Loretta, Yihad. Cómo se financia el terrorismo en la nueva economía. Barcelona, Ediciones Urano, 2004, 462 pp.Páginas 202-204Andrés Herrera FeligrerasPaksoy, H.B., How Governed, Who Pays? Lawrence (KS, Carrie, 2001, 79 pp.Páginas 204-206Dan KrejciReinares, Fernando, Terrorismo Global. Madrid, Taurus, 2003, 180 pp.Páginas 206-208Marcela Iglesias OnofrioSala Rose

  3. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Joaquín Piñeiro Blanca

    2009-12-01

    Full Text Available Affaya, Noureddine; Guerraoui, Driss, La imagen de España en Marruecos. Barcelona, Fundación CIDOB, 2005, 222 pp.Páginas 197-198Joaquín Piñeiro BlancaBengoa, José, Historia de un conflicto: El Estado y los mapuches en el siglo XX. Santiago, Planeta/Ariel, 1999, 237 pp.Páginas 198-201Danny Monsálvez AranedaBernstein, Richard J., El abuso del mal. La corrupción de la política y la religión desde el 11/9. Buenos Aires, Katz Editores, 2006, 228 pp.Páginas 201-202Alejandro Piñero GonzálezCarroll, James, La casa de la guerra. El Pentágono es quien manda. Barcelona, Crítica, 2007, 996 pp.Páginas 203-205David Molina RabadánEsparza, José Javier, Los ocho pecados capitales del arte contemporáneo. Córdoba, Ed. Almuzara, 2006, 172 pp.Páginas 205-206Juan José López CabralesFerguson, Niall, Coloso. Auge y decadencia del imperio americano. Barcelona, Debate, 2005, 504 pp.Páginas 206-208David Molina RabadánFernández Navarro, Antonio, Sindicatos y empresarios almerienses ante la construcción de la democracia. Almería, Instituto de Estudios Almerienses-Diputación de Almería, 2005, 263 pp.Páginas 209-211Áurea Vidal GómezGraham, Helen, La República española en guerra (1936-1939. Barcelona, 2006, 611 pp.Páginas 211-214Carmen González MartínezHerrerín López, Ángel, La CNT durante el franquismo. Clandestinidad y exilio (1939-1975. Madrid, Siglo XXI, 2004, 468 pp.Páginas 214-216Alberto Carrillo LinaresHyde-Price, Adrian, European Security In The Twenty First Century. The Challenge Of Multipolarity. Oxon, Routledge, 2007, 241 pp.Páginas 216-218Javier Lión BustilloOrellana, Luis, El fuego y la nieve. Historia del movimiento pentecostal en Chile: 1909-1932. Concepción, CEEP Ediciones, 2006, 165 pp.Páginas 218-220David Oviedo SilvaQuiroga Fernández de Soto, Alejandro, Los orígenes del nacionalcatolicismo. José Pemartín y la Dictadura de Primo de Rivera. Granada, Editorial Comares, 2006, 154 pp.Páginas 221-223Miguel

  4. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Alejandro Estrella González

    2008-12-01

    Full Text Available Aragüés, Juan Manuel, Líneas de fuga. Filosofía contra la sociedad idota. Madrid, Fundación de Investigaciones Marxistas, 2002, 172 pp.Páginas 183-184Alejandro Estrella GonzálezArroyo Martín, José Víctor, La Banca en España en el período de entreguerras, 1920-1935. Un modelo de modernización y crecimiento. Bilbao, Archivo Histórico BBVA, 2003, 303 pp.Páginas 184-186José Víctor Arroyo MartínBuriano Castro, Ana (ed., Tras la Memoria. El Asilo Diplomático en Tiempos de la Operación Cóndor. México D.F., Instituto Mora, Instituto de Cultura de la Ciudad de México, 2000, 291 pp.Páginas 186-188Jesús Fernández GarcíaCuenca Toribio, José Manuel, Conversaciones con Alfonso Armada: el 23F. Madrid, Actas, 2001, 254 pp.Páginas 188-190David Molina RabadánHijano del Río, Manuel; Ruiz Romero, Manuel, Documentos para la historia de la autonomía andaluza (1882-1982. Málaga, Sarriá, 2001, 650 pp.Páginas 190-192Carlos Alberto Chernichero DíazIrujo, José María, La lista negra. Los espías nazis protegidos por Franco y la Iglesia. Madrid, Aguilar, 2003, 2ª ed., 254 pp.Páginas 192-194Julio Pérez SerranoNye, Joseph S., La paradoja del poder norteamericano. Madrid, Taurus, 2003, 303 pp.Páginas 195-196Marcela Iglesias OnofrioPopovich, Pavel (ed., Orbits of peace and progress. Moscú, Mir Publishers, 1988, 304 pp.Páginas 196-199Rafael Gómez SánchezRodríguez Jiménez, José Luis, Los esclavos españoles de Hitler. Barcelona, Planeta, 2002, 321 pp.Páginas 199-201Gonzalo Ruiz BidónRufin, Jean-Christophe, Globalia. Paris, Editions Gallimard, 2003, 495 pp.Páginas 201-203Jean Marie IzquierdoSantacreu Soler, José Miguel; García Andreu, Mariano, La transició democràtica al País Valencià. Simat de la Valldigna, La Xara Edicions, 2002, 158 pp.Páginas 203-204Helena Ruiz CondeStiglitz, Joseph E., El malestar en la globalización. Madrid, Suma de Letras, 2003, 471 pp.Páginas 205-206Alfonso Galindo LucasTorres Villanueva, Eugenio

  5. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Diego Iturriaga Barco

    2009-12-01

    Full Text Available Beck, Ulrich, Sobre el terrorismo y la guerra. Barcelona, Paidós, 2002, 62 pp.Páginas 187-188Diego Iturriaga BarcoBurleigh, Michael, Sangre y rabia. Una historia cultural del terrorismo. Madrid, Taurus, 2008, 736 pp.Páginas 188-190David Molina RabadánColectivo IOÉ, Barómetro Social de España. Análisis del período 1994-2006. Madrid, Traficantes de Sueños y Centro de Investigación para la Paz (CIP-Ecosocial, 2008, 469 pp.Páginas 190-192Gema González FerreraDavis, Mike, Planeta de Ciudades Miseria. Madrid, Foca, 2007, 283 pp.Páginas 192-194Jesús Rodríguez GonzálezGonzález Madrid, Damián A. (coord., El Franquismo y la Transición en España: Desmitificación y Reconstrucción de la memoria de una época. Madrid, Catarata, 2008, 273 pp.Páginas 194-196Antonio Muñoz de Arenillas ValdésIllades, Carlos, Las otras ideas. El primer socialismo en México 1850-1935. UAM, Era. 2008, 327 pp.Páginas 196-199Alejandro Estrella GonzálezOrtega López, Teresa María (ed., Por una Historia Global. El debate historiográfico en los últimos tiempos. Granada, Universidad de Granada, Prensas Universitarias de Zaragoza, 2007, 440 pp.Páginas 199-201Francisco de Paula Villatoro SánchezOrtiz Heras, Manuel (coord., Movimientos sociales en la crisis de la dictadura y la transición: Castilla-La Mancha, 1969-1979. Ciudad Real, Almud Ediciones, 2008, 337 pp.Páginas 201-203Antonio Muñoz de Arenillas ValdésRevista de Estudios Europeos, 46-47 (Mayo/Diciembre 2007Páginas 204-206Miguel Ángel González ClarósTitos Martínez, Manuel, Música y finanzas. Biografía económica de Manuel de Falla. Granada, Publicaciones del Archivo Manuel de Falla, 2008, 473 pp.Páginas 206-209Joaquín Piñeiro BlancaSloterdijk, Peter, En el mundo interior del Capital. Para una teoría filosófica de la globalización. Madrid, Siruela, 2007, 336 pp.Páginas 209-212José Antonio Ruiz GilWeiner, Tim, Legado de cenizas. Historia secreta de la CIA. Barcelona, Debate, 2008, 718

  6. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Juan Gustavo Núñez Olguín

    2009-12-01

    Full Text Available Amorós, Mario, Compañero Presidente. Salvador Allende, una vida por la democracia y el socialismo. Valencia, Universitat de València, 2008, 372 pp.Páginas 215-217Juan Gustavo Núñez OlguínCoppola Salvatore, El pontífice sumamente bondadoso. Diplomazia, chiesa e politica ai tempi di Giovanni XXIII (1958-1963, Editan, Galatina, 2009Páginas 217-218Romina De CarliDel Arco Blanco, Miguel Ángel, Hambre de Siglos. Mundo rural y apoyos sociales del franquismo en Andalucía Oriental, 1936-1951. Granada, Comares, 2007, 513 pp.Páginas 218-220Damián González MadridGómez Morales, Jesús (Coord., La Gestión de Proyectos Europeos de Cooperación Transfronteriza. Cádiz, Universidad de Cádiz-Aula Universitaria del Estrecho, 2008, 483 pp.Páginas 220-221Héctor Vega DeloyaLacomba, Josep, Historia de las migraciones internacionales. Historia, geografía análisis e interpretación. Madrid, Catarata, 2008, 253 pp.Páginas 222-224Francisco de Paula Villatoro SánchezOliver Olmo, Pedro, La utopía insumisa de Pepe Beunza. Barcelona, Virus, 2002, 174 pp.Páginas 224-225María Francisca Fernández CáceresPiatigorsky, Jacques; Sapir, Jacques (coord., Le Grand Jeu: Les enjeux géopolitiques de l’Asie Centrale. Paris, Éditions Autrement, 2009, 252 pp.Páginas 225-227Manuel Pérez SalinasShapiro, Robert J., 2020: un nuevo paradigma. Cómo los retos del futuro cambiarán nuestra forma de vivir y trabajar. Barcelona, Ediciones Urano, 2009, 574 pp.Páginas 227-229David Molina RabadánVelarde, Juan, Cien años de economía española. Madrid, Ediciones Encuentro, 2009, 360 pp.Páginas 229-231Ubaldo Cuadrado MartínezVarios autores, Explosión urbana y globalización. Madrid, Editorial Popular, 2008, 185 pp.Páginas 231-232Miguel Ángel González ClarósVarios autores, Atlas Cronológico de la Historia de España. Madrid, Real Academia de la Historia-Grupo SM, 2007, 446 pp.Páginas 232-234Joaquín Piñeiro BlancaZakaria, Fareed, El mundo después de USA

  7. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Diego Iturriaga Barco

    2009-04-01

    Full Text Available Aznar López, José María, Ocho años de gobierno. Una visión personal de España. Barcelona, Planeta, 2004.Páginas 201-203Diego Iturriaga BarcoChamocho Cantudo, Miguel Ángel, La Justicia del Pueblo. Los tribunales populares de Jaén durante la Guerra Civil. Jaén, Instituto de Estudios Giennenses, 2004, 296 pp.Páginas 203-205Eduardo Cebreiros ÁlvarezCobo Romero, Francisco, Revolución campesina y contrarrevolución franquista en Andalucía. Granada, Universidad de Granada, Universidad de Córdoba, 2004, 396 pp.Páginas 205-207David Molina RabadánDay, Timothy, Un siglo de música grabada. Madrid, Alianza Editorial, 2002, 312 pp.Páginas 207-209Joaquín Piñeiro BlancaDos Santos, Theotonio, La teoría de la dependencia. Balance y perspectivas. México, Plaza & Janés, 2003, 170 pp.Páginas 209-213Andre Gunder FrankGoodman, Amy, En la cama con el enemigo. Contra los políticos untados de petróleo, los empresarios que se benefician de las guerras y los medios de comunicación que los encubren. Madrid, Temas de Hoy, 2004, 364 pp.Páginas 213-215Inmaculada Ramírez PérezGu, Zhibin, China's Global Reach. Markets, Multinationals, and Globalization. Binghamton, Haworth Press, 2005, 359 pp.Páginas 215-216Jorge Nascimento RodriguesJiménez Bautista, Francisco, Las gentes del área metropolitana de Granada. Relaciones, percepciones y conflictos. Granada, Universidad de Granada, Instituto de la Paz y los Conflictos, 2004, 872 pp.Páginas 217-218David Molina RabadánKaye, H.J., Los historiadores marxistas británicos: un análisis introductorio. Zaragoza, Prensas Universitarias, 1989, 240 pp.Páginas 218-220Alejandro Estrella GonzálezKüng, Hans, Libertad conquistada: memorias. Madrid, Trotta, 2003, 619 pp.Páginas 221-222Gonzalo Ruiz BidónLópez Martínez, Mario (dir., Enciclopedia de Paz y Conflictos. Granada, Editorial Universidad de Granada, Junta de Andalucía-Consejería de Educación y Ciencia, 1.228 pp.Páginas 222-225Francisco Jim

  8. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    José Miguel Delgado Idarreta

    2009-12-01

    Full Text Available Andrés Cabello, Sergio; Capellán de Miguel, Gonzalo; Fandiño Pérez, Roberto y Orduña Prada, Mónica, La transición a la democracia en La Rioja. Logroño, Gobierno de La Rioja, 2001, 291 pp.Páginas 199-200José Miguel Delgado IdarretaBeneyto, José María; Martín de la Guardia, Ricardo y Pérez Sánchez, Guillermo (dirs., Europa y Estados Unidos. Una historia de la relación atlántica en los últimos cien años. Madrid, Biblioteca Nueva, 2005, 356 pp.Páginas 201-203David Molina RabadánBosworth, Richard J. B., Mussolini’s Italy. Life under the Fascist Dictatorship 1915-1945. New York, Penguin, 2006, 692 pp.Páginas 203-205Jan NelisDávila A., Mireya; Fuentes S., Claudio (eds., Promesas de cambio. Izquierda y derecha en el Chile contemporáneo. Santiago de Chile, Editorial Universitaria, FLACSO, 2003, 189 pp.Páginas 205-208David Oviedo SilvaDelgado Idarreta, José Miguel; Andrés Cabello, Sergio, Actas del V Simposio de Historia Actual: La Rioja, España, Europa. Logroño, Instituto de Estudios Riojanos, 2006, 449 pp.Páginas 208-212Alfonso Pinilla GarcíaEvans, Richard J., La llegada del Tercer Reich. Barcelona, Península, 2005, 672 pp.Páginas 212-214Alejandro Piñero GonzálezFriedman, Thomas L., La Tierra es plana. Breve historia del mundo globalizado del siglo XXI. Madrid, Martínez Roca, 2006, 496 pp.Páginas 214-216David Molina RabadánGil Calvo, Enrique, El miedo es el mensaje. Riesgo, incertidumbre y medios de comunicación. Madrid, Alianza Editorial, 2004, 320 pp.Páginas 216-218Lucía Benítez EyzaguirreGoicovic Donoso, Igor, Entre el dolor y la ira. La venganza de Antonio Ramón Ramón. Chile, 1914. Osorno, Editorial Universidad de los Lagos, 2005, 188 pp.Páginas 218-221Alejandra Brito PeñaGriffin, Roger (ed., Fascism, Totalitarianism and Political Religion. New York, Routledge, 2005, 225 pp.Páginas 221-222Jan NelisMcNeill, John R., Algo nuevo bajo el Sol. Historia medioambiental del mundo en el siglo XX. Alianza

  9. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    David Oviedo Silva

    2009-12-01

    Full Text Available Aróstegui, Julio; Saborido, Jorge, El tiempo presente. Un mundo globalmente desordenado. Buenos Aires, Eudeba, 2005, 172 pp.Páginas 215-218David Oviedo SilvaDíaz, Lorenzo, 50 Años de TVE. Madrid, Alianza Editorial, 2006, 414 pp.Páginas 219-220Virginia Martín JiménezFernández Alles, José Joaquín, Las relaciones intergubernamentales en el Derecho Constitucional Español. Cádiz, Servicio de Publicaciones de la Universidad de Cádiz-Fundación Centro de Estudios Constitucionales 1812, 2007, 338 pp.Páginas 220-224Marcela Iglesias OnofrioHernández Sandoica, Elena; Baldó Lacomba, Marc y Ruiz Carnicer, Miguel Ángel, Estudiantes contra Franco (1939-1975. Oposición política y movilización juvenil. Madrid. La Esfera de los libros, 2007, 512 pp.Páginas 224-226Gonzalo Ruiz BidónJeffreys-Jones, Rhodri, Historia de los servicios secretos norteamericanos. Barcelona, Paidós, 392 pp.Páginas 226-228David Molina RabadánMartín Casares, Aurelia, Antropología del género. Culturas, mitos y estereotipos sexuales. Madrid, Ediciones Cátedra, 2006, 344 pp.Páginas 228-230Lucía Benítez EyzaguirreOrtega y Gasset, José, Historia como sistema. Obras Completas de José Ortega y Gasset. Madrid, Taurus, 2004-.Páginas 230-232Alfonso Pinilla GarcíaProcacci, Giuliano, Historia general del siglo XX. Barcelona, Crítica, 2005, 636 pp.Páginas 232-233Jesús María Ramos PérezReinert, Erik S., La globalización de la pobreza. Cómo se enriquecieron los países ricos… y por qué los países pobres siguen siendo pobres. Barcelona, Crítica, 2007, 370 pp.Páginas 233-235David Molina RabadánSaid, Edward W., Representaciones del intelectual. Barcelona, Editorial Debate, 2007, 142 pp.Páginas 235-237Joaquín Piñeiro BlancaSaviano, Roberto, Gomorra: un viaje al imperio económico y al sueño de poder de la Camorra. Madrid, Editorial Debate, 2007, 324 pp.Páginas 237-238Flavia Pascariello

  10. Diseño de una web 2.0 sobre el turismo cultural en la comarca del Garraf en el ámbito de las TIC

    OpenAIRE

    Hidalgo Álvarez, Raquel

    2009-01-01

    En la actualidad existen muchas páginas Web donde colectivos de gente, organizaciones o usuarios personales se dan a conocer. Para crear estas páginas actualmente se pueden encontrar muchas herramientas que permiten la creación de páginas personales con pocos pasos. Estas páginas son fruto de plantillas, que luego no pueden ser modificadas o muy poco dinámicas y muy similares entre ellas. Para la creación de esta Web se ha partido desde cero para obtener una Web lo ...

  11. Star-spot distributions and chromospheric activity on the RS CVn type eclipsing binary SV Cam

    Science.gov (United States)

    Şenavcı, H. V.; Bahar, E.; Montes, D.; Zola, S.; Hussain, G. A. J.; Frasca, A.; Işık, E.; Yörükoǧlu, O.

    2018-06-01

    Using a time series of high-resolution spectra and high-quality multi-colour photometry, we reconstruct surface maps of the primary component of the RS CVn type rapidly rotating eclipsing binary, SV Cam (F9V + K4V). We measure a mass ratio, q, of 0.641(2) using our highest quality spectra and obtain surface brightness maps of the primary component, which exhibit predominantly high-latitude spots located between 60° - 70° latitudes with a mean filling factor of ˜35%. This is also indicated by the R-band light curve inversion, subjected to rigourous numerical tests. The spectral subtraction of the Hα line reveals strong activity of the secondary component. The excess Hα absorption detected near the secondary minimum hints to the presence of cool material partially obscuring the primary star. The flux ratios of Ca II IRT excess emission indicate that the contribution of chromospheric plage regions associated with star-spots is dominant, even during the passage of the filament-like absorption feature.

  12. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    José Francisco Tinao Martín-Peña

    2008-12-01

    Full Text Available Chomsky, Noam, Poder y Terror. Reflexiones posteriores al 11/09/2001. Barcelona, RBA, 2003, 155 pp.Páginas 133-135José Francisco Tinao Martín-PeñaCobo Romero, Francisco, Conflicto rural y violencia política. Jaén, Universidad de Jaén-Universidad de Granada, 1998, 381 pp.Páginas 135-137José Luis Gutiérrez MolinaColacrai, Miryam (ed., Relaciones Internacionales. Viejos temas, nuevos debates. Rosario, Centro de Estudios en Relaciones Internacionales de Rosario, 2001, 203 pp.Páginas 137-141David Molina RabadánCuenca Toribio, José Manuel, Historia y actualidad. Clío en la posada. Madrid, Actas, 2002, 402 pp.Páginas 141-143Alejandro Román AntequeraHardt, Michael; Negri, Antonio, Imperio. Barcelona, Paidós, 2002, 432 pp.Páginas 143-145Jesús Fernández GarcíaHowson, Gerald, Armas para España. La historia no contada de la Guerra Civil Española. Barcelona, Península, 2000, 450 pp.Páginas 145-148Roberto Germán Fandiño PérezKaku, Michio, Visiones: cómo la ciencia revolucionará la materia, la vida y la mente en el siglo XXI. Madrid, Debate, 1998, 484 pp.Páginas 148-150Rafael Gómez SánchezLemus López, Encarnación, En Hamelin... la Transición española más allá de la frontera. Oviedo, Septem Ediciones, 2001, 158 pp.Páginas 150-153Julio Pérez SerranoSalazar Vergara, Gabriel, Labradores, peones y proletarios. Formación y crisis de la sociedad popular chilena del siglo XIX. Santiago de Chile, LOM Ediciones, 2000, 3ª ed., 334 pp.Páginas 153-155Leonardo Mazzei de GraziaSantacreu Soler, José Miguel, Peseta y política: Historia de la peseta, 1868-2001. Barcelona, Ariel Historia, 2002, 196 pp.Página 155Joaquín Piñeiro Blanca

  13. El Cine durante la Primera Guerra Mundia a través de las páginas del diario El Progreso de Tenerife

    Directory of Open Access Journals (Sweden)

    Orlando Betancor

    2013-10-01

    Full Text Available Este artículo analiza el tratamiento in­formativo dado a las películas cinematográfi­cas en las páginas del periódico El Progreso de Santa Cruz de Tenerife durante la Primera Guerra Mundial. Además, recoge los princi­pales acontecimientos cinematográficos que tuvieron lugar en Tenerife en este momento y su repercusión en este diario. Investiga el papel de los anuncios publicitarios de los loca­les de exhibición, editados en este periódico, que mostraron al público de la Isla estas obras de arte del cine mudo. Asimismo, describe los estrenos más destacados del incipiente cine español y los documentales realizados por los ejércitos británico y francés y exhibidas en las salas de proyección de Tenerife durante esta etapa.This article analyses the informative treatment given to the films on the pages of the newspaper El Progreso of Santa Cruz de Tenerife during the First World War. Besides, it collects the principal movie events that took place in Tenerife in this time and its repercus­sion on this diary. It investigates the role of the advertisements of the movie houses, edited in this journal, that showed these masterpieces of the silent film era. Likewise it describes the most outstanding releases of the incipient Spa­nish cinema and the documentary films made by the British and French Armies and exhibited in the cinemas of Tenerife in this moment.

  14. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Jesús María Ramos Pérez

    2009-12-01

    Full Text Available Alfaro Monsalve, Karen et al., Historia sociopolítica del Concepción contemporáneo: memoria, identidad y territorio. Santiago-Concepción, Universidad ARCIS-Ediciones Escaparate, 2006 (Taller de Ciencias Sociales “Luis Vitale”.Páginas 195-196Jesús María Ramos PérezCarroll, Tim, La Gran Evasión. La verdadera historia de la fuga más famosa de la Segunda Guerra Mundial. Barcelona, Puzzle Editorial, 2005, 352 pp.Páginas 197-201Daniel Francisco Álvarez EspinosaCerón Torreblanca, Cristian Matías, “La paz de Franco”, la posguerra en Málaga: desde los oscuros años 40 a los grises años 50. Málaga, Servicio de Publicaciones de la Universidad de Málaga, 2007, 408 pp.Páginas 201-203Joaquín Piñeiro BlancaChang, Ha Joon; Grabel, Ilene, Reivindicar el desarrollo. Un manual de política económica alternativa. Barcelona, Intermón Oxfam, 2006, 208 pp.Páginas 203-206Fernando López CastellanoDel Amo, Mercedes; García Rey, Marcos y Ortega, Rafael (eds., El 11-M en la prensa árabe. Sevilla, Mergablum, 2004, 136 pp.Páginas 206-208Diego Iturriaga BarcoHardt, Michael; Negri, Antonio, Imperio. Barcelona, Paidós, 2002, 432 pp.Páginas 208-213Flavia PascarielloJudt, Tony, Postguerra. Una historia de Europa desde 1945. Madrid, Taurus, 2006, 1212 pp.Páginas 213-215David Molina RabadánLópez Castillo, Antonio, El republicanismo de centro. El Partido Republicano Radical de Almería durante la Segunda República (1931-1936. Almería, Instituto de Estudios Almerienses, 2006, 229 pp.Páginas 215-217Mónica Fernández AmadorMarsi, Luca, Estrategias corporativas. Aspectos sociales y consecuencias para la comunidad. Nanterre, Centre de Recherches Ibériques et Ibéro-Américaines, Universidad de Paris X–Nanterre, 2006, 295 pp.Páginas 217-219Alvar De la LlosaMorgan Philip, The Fall of Mussolini. Oxford-New York, Oxford University Press, 2007, 263 pp.Páginas 219-220Jan NelisPisanty Valentina, La difesa della razza. Antologia 1938-1943, con un

  15. Reseñas de Libros

    Directory of Open Access Journals (Sweden)

    Joaquín Piñeiro Blanca

    2009-12-01

    Full Text Available Álvarez Rey, Leandro (Estudio preliminar, recopilación y edición, Diego Martínez Barrio. Palabra de republicano. Sevilla, Ayuntamiento de Sevilla-Instituto de la Cultura y las Artes, 2007, 1.046 pp.Páginas 177-179Joaquín Piñeiro BlancaBancalari Molina, Alejandro, Orbe romano e Imperio Global. La romanización desde Augusto a Caracalla. Santiago de Chile, Editorial Universitaria, 2007, 330 pp.Páginas 179-182Francisco de Paula Villatoro SánchezBernal-Meza, Raúl y Saha, Suranjit (comps., Economía mundial y desarrollo regional. Buenos Aires, GEL, 2005, 403 pp.Páginas 182-184David Molina RabadánDavis, Mike, Planeta de Ciudades Miseria. Madrid, Foca Editorial, 2007, 283 pp.Páginas 184-185Daniel Alcalde GüelfoElguero Olavide, Ignacio, Los niños de los Chiripitifláuticos: retrato generacional de los nacidos en los 60. Madrid, La Esfera de los Libros, 2006, 672 pp.Páginas 185-189Daniel Francisco Álvarez EspinosaEstulin, Daniel, Los secretos del Club Bilderberg. Editorial Planeta, Barcelona, 2006, 304 pp.Páginas 190-191Mª del Rocío Piñeiro ÁlvarezFernández Bessa, Cristina et al., Frontera Sur. Nuevas políticas de gestión y externalización del control de la inmigración en Europa. Bilbao, Virus Editorial, 2008, 272 pp.Páginas 191-192Manuel Baraja EscuderoGonzález Clavero, Mariano; Pelaz Pérez, José Vidal; Pérez López, Pablo, Castilla y León en democracia. Partido, elecciones y personal político. 1977-2007. Salamanca, Junta de Castilla y León, 2007, 345 pp.Páginas 192-194Francisco de Paula Villatoro SánchezKhanna, Parag, El segundo mundo. Barcelona, Paidós, 2008, 570 pp.Páginas 194-196David Molina RabadánSanmartín Barros, Israel, Entre dos siglos. Globalización y pensamiento único. Madrid, Ediciones Akal, 2007, 334 pp.Páginas 196-198Joaquín Piñeiro BlancaToscano Gil, Francisco, Autonomía y potestad normativa local. Granada, Comares. 2007, 202 pp.Páginas 198-200José Joaquín Fernández AllesVV.AA., Ernesto

  16. ginas iniciais

    Directory of Open Access Journals (Sweden)

    Ficha catalográfica e sumário bilíngue

    2013-12-01

    Full Text Available REDES, Santa Cruz do Sul, v. 18, nº 3, p. 1 - 273, set/dez. 2013. ISSN: 1982-6745 Editores Virginia Elisabeta Etges Silvio Cezar Arend Sumário/Summary O PROCESSO DE MODERNIZAÇÃO AGRÍCOLA E AS TRAJETÓRIAS E ESTRATÉGIAS EMPREENDIDAS PELOS PRODUTORES AGRÍCOLAS DO MUNICÍPIO DE PASSO FUNDO – RS – BRASIL AGRICULTURAL MODERNIZATION PROCESS AND THE TRAJEÓRIAS AND STRATEGIES OF AGRICULTURAL PRODUCERS TAKEN BY THE CITY OF STEP FUND – RS –BRASIL Luiz Fernando Fritz Filho Lovois de Andrade Miguel Karen Beltrame Becker Fritz ANÁLISE ESPACIAL DA PRODUTIVIDADE DA AGROPECUÁRIA NO ESTADO DO PARANÁ – 1970, 1996 E 2007 SPATIAL ANALYSIS OF AGRICULTURAL PRODUCTIVITY IN THE STATE OF PARANA - 1970, 1996 AND 2007 Lucir Reinaldo Alves Andreice Criscieli Saran Aneli O ESVAZIAMENTO DAS REGIÕES RURAIS: O CASO DA BACIA LEITEIRA DE PIRACANJUBA – GO – BRASIL (2000-2010 THE EXODUS OF RURAL AREAS. THE CASE PIRACANJUBA – GOIÁS – BRAZIL (2000-2010 Margot Riemann Costa e Silva Luis Antonio Estevam A CONTRIBUIÇÃO DA AGRICULTURA FAMILIAR PARA O DESENVOLVIMENTO RURAL DE MATO GROSSO DO SUL - BRASIL THE CONTRIBUTION OF THE FAMILY FARM FOR RURAL DEVELOPMENT IN MATO GROSSO DO SUL STATE - BRAZIL Adriana Rita Sangalli Madalena Maria Schlindwein PEDAGOGIA DA ALTERNÂNCIA E REPRESENTAÇÕES SOCIAIS DE MEIO AMBIENTE EM DOCENTES E DISCENTES DE CASAS FAMILIARES RURAIS ALTERNATION PEDAGOGY AND SOCIAL REPRESENTATIONS OF THE ENVIRONMENT ON TEACHERS AND STUDENTS OF RURAL FAMILY HOUSES Edival Sebastião Teixeira Ederson Henrique de Souza Machado Fernanda Luiza Algeri Raffael Cantú BENEFÍCIOS DOS ARRANJOS PRODUTIVOS LOCAIS: A PERCEPÇÃO DOS APICULTORES SOBRE A REDE ABELHA CEARÁ - BRASIL BENEFITS OF LOCAL PRODUCTIVE ARRANGEMENTS (LPA: PERCEPTIONS OF THE MEMBERS OF THE APICULTURE NETWORK ‛REDE ABELHA CEARÁ - BRAZIL Oderlene Vieira de Oliveira Francisco Correia de Oliveira Rosângela Andrade Pessoa QUALIDADE E INOVAÇÃO EM SERVIÇOS DE TRANSPORTE COLETIVO PÚBLICO EM GOIÂNIA – GO – BRASIL QUALITY AND INNOVATION IN PUBLIC TRANSPORTATION SERVICE IN GOIANIA – GO – BRAZIL Maria Regina Silva Lima Bento Alves Costa Filho Cesar Ricardo Maia Vasconcelos CRESCIMENTO OU MANUTENÇÃO DA POSIÇÃO: O DILEMA DOS GESTORES DE UMA INDÚSTRIA DE PLÁSTICOS DA SERRA GAÚCHA GROWTH OR MAINTENANCE OF THE POSITION: THE DILEMMA OF THE MANAGERS OF A PLASTICS INDUSTRY SERRA GAUCHA Julio Cesar Ferro de Guimarães Eliana Andréa Severo Sylvia Maria Azevedo Roesch Pelayo Munhoz Olea Eric Charles Henri Dorion ESTRATÉGIA E COMUNICAÇÃO COM O MERCADO. PERCEPÇÃO DOS GESTORES DAS INSTITUIÇÕES DE ENSINO SUPERIOR SOBRE AS AÇÕES DE RESPONSABILIDADE SOCIAL NA PERSPECTIVA DO MÉTODO FENOMENOLÓGICO STRATEGY AND COMMUNICATION WITH THE MARKET. PERCEPTION OF MANAGERS OF INSTITUTIONS OF HIGHER EDUCATION ON THE CORPORATE SOCIAL RESPONSIBILITY IN THE PHENOMENOLOGICAL METHOD VIEW Roberto Bazanini Ademir Antonio Ferreira Homero Leoni Bazanini Rosalina Florindo da Silva REGIONAL DEVELOPMENT POLICY IN BRAZIL: A REVIEW OF EVALUATION LITERATURE POLÍTICA DE DESENVOLVIMENTO REGIONAL NO BRASIL: UMA REVISÃO DA LITERATURA SOBRE AVALIAÇÃO Guilherme Mendes Resende FLORESTAS E PARTICIPAÇÃO COMO VETORES DO DESENVOLVIMENTO REGIONAL: REFLEXÕES SOBRE OS CASOS ACRE E RIO GRANDE DO SUL FORESTRY AND CIVIC PARTICIPATION AS DRIVERS OF REGIONAL DEVELOPMENT: ANALYSIS OF THE CASES ACRE AND RIO GRANDE DO SUL Markus Erwin Brose A IMPORTÂNCIA DA INTERDISCIPLINARIDADE PARA COMPREENSÃO DAS QUESTÕES AMBIENTAIS THE IMPORTANCE OF INTERDISCIPLINARITY FOR UNDERSTADING OF THE ENVIRONMENTAL SUBJETCTS Cidonea Machado Deponti PROJETOS DE VIDA, MOBILIDADE E METRÓPOLE: AS TRAJETÓRIAS DE JOVENS ADULTOS NO CONTEXTO INTERNACIONAL LIFE PROJECTS, MOBILITY AND METROPOLIS : YOUNG ADULTS’ IN THE INTERNATIONAL CONTEXT Grazielle Betina Brandt Serge Côté

  17. ginas iniciais

    Directory of Open Access Journals (Sweden)

    Ficha catalográfica e sumário bilíngue

    2013-09-01

    Full Text Available REDES, Santa Cruz do Sul, v. 18, nº 2, p. 1 - 273, maio/ago. 2013. ISSN: 1982-6745 Editores Virginia Elisabeta Etges Silvio Cezar Arend Sumário/Summary A EFETIVIDADE DA COLABORAÇÃO ENTRE ORGANIZAÇÕES DO ARRANJO PRODUTIVO LOCAL (APL: EXPERIÊNCIAS DOS PROCESSOS LOGÍSTICOS NAS INDÚSTRIAS DO VALE DA ELETRÔNICA DE MINAS GERAIS – BRASIL THE EFFECTIVENESS OF COLLABORATION INTER-COMPANIES: EXPERIENCES OF LOGISTIC PROCESSES IN THE INDUSTRIES OF THE ELECTRONIC VALLEY OF MINAS GERAIS – BRAZIL Ricardo Silveira Martins Osmar Vieira de Souza Filho IDENTIFICAÇÃO DE CLUSTERS INDUSTRIAIS: UMA APLICAÇÃO DE ÍNDICES DE ESPECIALIZAÇÃO E CONCENTRAÇÃO, E ALGUMAS CONSIDERAÇÕES IDENTIFICATION OF INDUSTRIAL CLUSTERS: AN APPLICATION OF INDICES OF SPECIALIZATION AND CONCENTRATION, AND SOME CONSIDERATIONS Autenir Carvalho de Rezende Bernardo Palhares Campolina Diniz CAPACIDADES LOCAIS: POR UMA NOVA ECONOMIA DOS TERRITÓRIOS PRODUTIVOS LOCAL CAPACITIES: FOCUSING ON A NEW ECONOMY FOR PRODUCTIVE TERRITORIES Giancarlo Corò Paolo Gurisatti ANÁLISE CRÍTICA DA INFLUÊNCIA DO CAPITALISMO TRANSNACIONAL SOBRE AS INSTITUIÇÕES E ORGANIZAÇÕES CRITICAL ANALYSIS OF THE INFLUENCE OF TRANSNATIONAL CAPITALISM ON INSTITUTIONS AND ORGANIZATIONS José G. Vargas-Hernández REDES SOCIAIS E GOVERNANÇA DEMOCRÁTICA: O CASO DE UMA INSTITUIÇÃO FEDERAL DE ENSINO SUPERIOR DE MINAS GERAIS – BRASIL NETWORKS AND DEMOCRATIC GOVERNANCE: THE CASE OF A PUBLIC INSTITUTION OF HIGHER EDUCATION OF MINAS GERAIS – BRAZIL Edimilson Eduardo da Silva Fábio Costa Lasmar José Roberto Pereira DE MATRIZ CONSERVADORA A UMA POSTURA PROGRESSISTA: CATOLICISMO SOCIAL NO RIO GRANDE DO SUL (RS - BRASIL FROM A CONSERVATIVE MATRIX TO A PROGRESSIVE ATTITUDE: SOCIAL CATHOLICISM IN RIO GRANDE DO SUL (RS - BRAZIL Olgário Paulo Vogt Roberto Radünz DO PREJUÍZO À TRANSGRESSÃO: O DISCURSO SOBRE O MOVIMENTO GREVISTA NA IMPRENSA INJURY TO THE TRANSGRESSION: THE DISCOURSE ON STRIKE IN THE PRESS Patrícia Regina Schuster Ângela Cristina Trevisan Felippi PADRONIZAÇÃO DE PRODUTOS DAS AGROINDÚSTRIAS RURAIS E A INSERÇÃO NOS MERCADOS PRODUCT STANDARDIZATION AND INTEGRATION OF RURAL AGRIBUSINESS MARKETS Silvio Santos Junior Augusto Fischer Simone Sehnem Paulo Dabdab Waquil Miguelangelo Gianezini O MERCADO DE TRABALHO FORMAL NO SETOR SUCROALCOOLEIRO NO BRASIL FORMAL LABOR MARKET IN THE BRAZILIAN SUGARCANE AGRO-INDUSTRY Carlândia Brito Santos Fernandes Pery Francisco Assis Shikida Marina Silva da Cunha TRANSIÇÕES POPULACIONAIS E VULNERABILIDADE ÀS MUDANÇAS CLIMÁTICAS NO BRASIL POPULATION TRANSITIONS AND VULNERABILITY TO CLIMATE CHANGE IN BRAZIL Alisson Flávio Barbieri CRESCIMENTO ECONÔMICO BRASILEIRO E EMISSÃO DE CO2 BRAZILIAN ECONOMIC GROWTH AND THE EMISSION OF CO2 Cleyzer Adrian Cunha Paulo Roberto Scalco PARQUES URBANOS MUNICIPAIS EM DOURADOS - MS -BRASIL: ESTADO DA ARTE URBAN MUNICIPAL PARKS IN DOURADOS – MS – BRAZIL: THE STATE OF THE ART Maria Cristiane Fernandes da Silva Lunas Lidia Maria Lopes Rodrigues Ribas AS FINANÇAS DOS MUNICÍPIOS PARAENSES: ENSAIO ANALÍTICO SOBRE AS RECEITAS DE TRANSFERÊNCIAS E DE SUAS PRINCIPAIS DESPESAS SOCIAIS FINANCES OF PARÁ MUNICIPALITIES: ANALYTICAL ESSAY ABOUT REVENUES OF TRANSFER AND ITS MAIN SOCIAL SPENDING José Raimundo Barreto Trindade Wesley Pereira Oliveira

  18. Información del conocimiento organizacional a través de los informes anuales publicados en las páginas web de las empresas

    Directory of Open Access Journals (Sweden)

    Tejedo Romero, Francisca

    2014-03-01

    Full Text Available Internet is a means of communication and transmission of information that has led companies to develop their business activities in knowledge-based economies. Knowledge has become the most important resource for creating competitive advantages, specifically the knowledge incorporated by companies into their processes, systems, culture, R&D, i.e., their organizational knowledge (Structural Capital. Therefore, Structural Capital disclosures by companies can improve their reputation. Thus, the objective of our work has focused on analysing the information on organizational knowledge supplied by companies in annual reports posted on their websites, as well as on its evolution over the years studied.Internet es un medio de comunicación y transmisión de información que ha dado lugar a que las empresas desarrollen su actividad en economías basadas en el conocimiento. El conocimiento se ha convertido en el recurso más importante para la creación de ventajas competitivas, en particular el conocimiento que las empresas han incorporado en sus procesos, sistemas, cultura, I+D, es decir, su conocimiento organizacional (Capital Estructural. Por ello, la publicación de información por parte de las empresas sobre su Capital Estructural puede mejorar la reputación de las mismas. Así, el objetivo de este trabajo se ha centrado en analizar la cantidad de información sobre el conocimiento organizacional que suministran las empresas en los informes anuales obtenidos de sus páginas web, así como la evolución sufrida en los años objeto de estudio.

  19. Estudio de metales pesados y arsénico en los suelos de olivar de Sierra Mágina, Jaén (España

    Directory of Open Access Journals (Sweden)

    Juan Carlos Menjivar Flores

    2009-10-01

    Full Text Available En Sierra Mágina, Jaén (España fueron seleccionados 35 suelos dedicados al cultivo del olivar en los que se estudió la concentración de cromo (Cr, níquel (Ni, cobre (Cu, plomo (Pb, Zinc (Zn y arsénico (As a profundidades entre 0 - 20 y 20 - 40 cm. Estos suelos se desarrollan sobre materiales carbonatados, predominando Calcisoles pétricos y Regosoles calcáricos (FAO, 1999. La homogeneidad del material parental es responsable de la ausencia de variaciones significativas en las propiedades de los suelos a las profundidades estudiadas, así como en el contenido de metales pesados y arsénico. El Cu es el único elemento que varía con la profundidad (p < 0.01, presentando un valor promedio más elevado entre 0 y 20 cm debido a la aplicación de controles fitosanitarios en los cultivos de la región. En los suelos con texturas arcillosa y limosa se presentan concentraciones más altas de Cr y Ni, mientras que el resto de metales analizados y el As no presentan variaciones significativas con las principales propiedades físicas y químicas de los suelos. Los elementos estudiados no exceden el nivel de referencia establecido para suelos por la Junta de Andalucía y presentan concentraciones similares a las encontradas en rocas equivalentes y en suelos no contaminados. Los valores promedio en As, Cr, Cu, Ni, Pb y Zn están relacionados con las concentraciones heredadas del material litológico del que se derivan.

  20. Estudio de metales pesados y arsénico en los suelos de olivar de Sierra Mágina, Jaén (España

    Directory of Open Access Journals (Sweden)

    Menjivar Flores Juan Carlos

    2009-12-01

    Full Text Available En Sierra Mágina, Jaén (España fueron seleccionados 35 suelos dedicados al cultivo del olivar en los que se estudió la concentración de cromo (Cr, níquel (Ni, cobre (Cu, plomo (Pb, Zinc (Zn y arsénico (As a profundidades entre 0 - 20 y 20 - 40 cm. Estos suelos se desarrollan sobre materiales carbonatados, predominando Calcisoles pétricos y Regosoles calcáricos (FAO, 1999. La homogeneidad del material parental es responsable de la ausencia de variaciones significativas en las propiedades de los suelos a las profundidades estudiadas, así como en el contenido de metales pesados y arsénico. El Cu es el único elemento que varía con la profundidad (p < 0.01, presentando un valor promedio más elevado entre 0 y 20 cm debido a la aplicación de controles fitosanitarios en los cultivos de la región. En los suelos con texturas arcillosa y limosa se presentan concentraciones más altas de Cr y Ni, mientras que el resto de metales analizados y el As no presentan variaciones significativas con las principales propiedades físicas y químicas de los suelos. Los elementos estudiados no exceden el nivel de referencia establecido para suelos por la Junta de Andalucía y presentan concentraciones similares a las encontradas en rocas equivalentes y en suelos no contaminados. Los valores promedio en As, Cr, Cu, Ni, Pb y Zn están relacionados con las concentraciones heredadas del material litológico del que se derivan.

  1. GeoCensus : extracção de informação geográfica da Web

    OpenAIRE

    Macedo, Joaquim; Santos, Maribel Yasmina

    2002-01-01

    O sistema GeoCensus tem como objectivo a extracção de informação geográfica da Web. Através da colecta exaustiva (para já apenas uma amostra significativa) de páginas Web existentes em Portugal, o sistema localiza geograficamente os servidores que hospedam as páginas colectadas. A localização geográfica das páginas pode ser realizada usando diferentes fontes de informação, nomeadamente a localização geográfica dos servidores Web que hospedam as páginas, os nomes geográficos extraí...

  2. Bibliografía

    Directory of Open Access Journals (Sweden)

    Hermes Tovar P.

    1972-01-01

    Full Text Available MARGARITA GONZÁLEZ. El Resguardo en el Nuevo Reino de Granada. Bogotá, 1970, 197 páginas. PEÑUELA, CAYO LEÓNIDAS: Álbum de Boyacá, 2 tomos, segunda edición, Tmi- Ja, 1969-1970, 764 -f- 535 páginas. Fotografías. WEST, ROBERT C: La minería de aluvión en Colombia durante el período Colo- nial, Divulgación Cultural, Universidad Nacional de Colombia, Bogotá, 1972, 129 páginas, XVI B. Láminas. (Traducción de Jorge Orlando Meló y re- visión de Camilo Domínguez. URRUTIA, MIGUEL y ARRUBLA, MARIO (editores: Compendio de Estadísticas Históricas de Colombia, Dirección de Divulgación Cultural, Universidad Na- cional de Colombia, Bogotá, 1970, 314 páginas. MARTÍNEZ ZÜLAICA, ANTONIO: La Medicina del siglo XVIII en el Nuevo Reino de Granada, Universidad Pedagógica y Tecnológica de Colombia^ Tun- ja, 1972, 318 páginas. RUIZ RIVERA, JULIÁN: Fuentes para la Demografía Histórica de Nueva Gra- nada, Escuela de Estudios Hispanoamericanos, Sevilla, 1972, 157 páginas, 1 mapa. GILHODES, FIERRE: Las luchas agrarias en Colombia, Ed. El Tigre de Papel, Bogotá-Medellín, 1972, 90 páginas. (Traducción, Jorge Orlando Melo.

  3. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1943-06-01

    Full Text Available British Medical Information Service. 3, Hanover Street. London, w. 1. Autores, Batchelor, R. C. L., Murrell, M., Thomson, G. M. Revista. British Journal of Venereal Diseases. Tomo 17, páginas 244-9. Julio y Octubre, 1941. Sulfatiazol en el tratamiento de la gonorrea / Scarff, R. ,W. and Smith, C. P. Revista : Brttish Journal. Abreviación: Brit. J. Sur. Tomo 29. Páginas 393-396. Fecha: Abril, 1942. Lesiones proliferativas y otras lesiones de mama en varón / Hemphill, R. E. Revista Jonrnal of Mental Science. Tomo 88. páginas 1-30. Enero, 1942. Catatonia hipertiroidea: un compiejo de síntomas esqulzofrenicos / Miller, E. W., Pybus, F. C. Revista: Journal of Pathology and Bacteríology, Abreviación : J. Path. Bact. Tomo 54. Páginas 155-168. Fecha: Abril-1942. El efecto de la estrona sobre ratones de tres cepas puras, con especial referencia a las glandulas mamarias / Dann, L., Glucksmann, A. Revísta : Lancet. Tomo 1. Páginas 95-98. Fecha, 24/1/42. Heridas experimentaleis tratadas con aceite de higado de bacalao y sustancias afines / Maitland, A. J. L. Revista: Journal of the Royal Naval Medical Service. Abreviación: J. roy. nav. med. Serv. Tomo 28. Páginas 3-17. Fecha: Enero, 1942. Quemaduras de guerra. Un estudio del tratamiento y resultados en un centenar de casos / Sheehan. H. L. Revista: Lancet. Tomo 1. Páginas: 616-618. Fecha: 23/5/42. Transfusión de sangre para  hemorragia obstetrica y shock / Carter, A. B. Revista, Journal of Mental Science. Tomo 88, páginas 31-81. Enero, 1942. Los factores pronosticos en las psicosis de los adolescentes

  4. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1943-05-01

    Full Text Available British Medical Information Service. 3, Hanover Street. London, w. 1. Autores: Robson, J. M,., Tebrich, W. Revista: British Medical Journal. Abreviación: Brit. Med. J. Tomo 1. Páginas 687-690. Fecha, 6/6/42. Penetración y distribución de sodio-sulfacetamida en  el  tejido ocular de los conejos / Autores: Henson, R. R., Russell, D. S. Revista: Journal of Pathology and Bacteriology. Abreviación: J. Path. Bact. Tomo 54. Páginas 227-234. Fecha: Abril, 1942. Leuco-encefalitis hemorragica aguda: historia de un caso / Autores: Whitby, r, E. H. Revista: Lancet. Tomo 1. Páginas 581-584. Fecha, 16/5/42. Riesgos de la transfusión /  Autores: Wíddowsou, E. M., l\\lcCance R. A. Revista: Lancet. Tomo 1. Páginas 588-590. Fecha 1615142. El recambio del hierro en los adultos con dietas de pan blanco y moreno /  Autores: Albert, M. Rlevista: Brltísh Medlcal Journal. Abreviación: Brit; Med. J. Tomo 1. Páginas 666-668. Fecha, 30/5/42. Calcificación de los discos intervertebrales /  Autores: Findlay, G. M. Revista: Proceedings of the Royal Society of Medicine. Abreviación: Proc. royo Soco Med. Tomo 35. Páginas 157-160. Fecha, Diciembre, 1941. Investigaciones de laboratorio sobre tifus / Autores: Cheeseman, E. A. Revista: .Journal of Hygiene, Abreciación: J. Byg., Cambo Tomo 41. Páginas 463-472. Fecha, Diciembre, 1941. Comparación entre el riesgo profesional directo e indirecto en la mortalidad por tuberculosis pulmonar /  Autores: Simpson, K. Revlsta : Lancet. Tomo 1. Páginas 697-698. Fecha 13/6/42. Accidentes producidos por el aire durante la transfusión /  Autores: Young, J. Revista: .Iournal of Obstetric & Gynecology or the British Empíre. Abreviación: .J. Obstet. Gynaec. Brit. Emp. Tomo 49. Páginas 221-246. Fecha, Junio, 1942. El fenómeno eclampsico, y la isquemia placentaria /  Autores: Menzies, M. F. Revista : Lancet. Tomo 2. Páginas 35-38. Fecha: 11/7/42. Parto en el hospital o en el propio domicilio / Autores: Findlay

  5. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1942-11-01

    Full Text Available Autores, Foster-Carter, A. F. Revista, British Journal of Tuberculosis. Tomo 36, páginas 19-38. Enero, 1942. La anatomia del arból bronquial / Autores, Robinson, M. Revista, Archives of Disease in Childhood. Tomo 17, páginas 23-29. Marzo, 1942. Una nueva investigación sobre la crianza de niños al pecho /  Autores, Medawar, P. B. Revista, Lancet. Tomo 1, páginas 350-352. Fecha 21/3/42. Coagulantes químicos en el tratamiento de las quemaduras / Autores, Wyburn-Mason, R. Revista, Lancet. Tomo 2, páginas 662-3. Fecha 29/11/41. Neuritis braquial en forma epidemica

  6. Precise determination of protein extinction coefficients under native and denaturing conditions using SV-AUC.

    Science.gov (United States)

    Hoffmann, Andreas; Grassl, Kerstin; Gommert, Janine; Schlesak, Christian; Bepperling, Alexander

    2018-04-17

    The accurate determination of protein concentration is an important though non-trivial task during the development of a biopharmaceutical. The fundamental prerequisite for this is the availability of an accurate extinction coefficient. Common approaches for the determination of an extinction coefficient for a given protein are either based on the theoretical prediction utilizing the amino acid sequence or the photometric determination combined with a measurement of absolute protein concentration. Here, we report on an improved SV-AUC based method utilizing an analytical ultracentrifuge equipped with absorbance and Rayleigh interference optics. Global fitting of datasets helped to overcome some of the obstacles encountered with the traditional method employing synthetic boundary cells. Careful calculation of dn/dc values taking glycosylation and solvent composition into account allowed the determination of the extinction coefficients of monoclonal antibodies and an Fc-fusion protein under native as well as under denaturing conditions. An intra-assay precision of 0.9% and an accuracy of 1.8% compared to the theoretical value was achieved for monoclonal antibodies. Due to the large number of data points of a single dataset, no meaningful difference between the ProteomeLab XL-I and the new Optima AUC platform could be observed. Thus, the AUC-based approach offers a precise, convenient and versatile alternative to conventional methods like total amino acid analysis (AAA).

  7. The magazine Vida e Saúde: manners of educating and looking at the feminine body in its pages (1940–1950 La revista Vida e Saúde: modos de mirar y educar el cuerpo femenino en sus páginas (1940-1950 A REVISTA VIDA E SAÚDE:MODOS DE OLHAR E EDUCAR O CORPO FEMININO EM SUAS PÁGINAS (1940-1950

    Directory of Open Access Journals (Sweden)

    André Dalben

    2008-12-01

    -tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} A revista Vida e Saúde sustentou durante a década de 1940 uma educação do corpo pautada nos discursos sobre a saúde e a beleza feminina construída a partir de três instituições: a científica, a religiosa e a política. Procurando compreender como se organizava nas páginas da revista essa proposta, explicitamos os diferentes dispositivos utilizados para sustentar os modos sobre como se deu a educação do corpo feminino pelas páginas da referida revista.

  8. The mechanism of thioacetamide-induced apoptosis in the L37 albumin-SV40 T-antigen transgenic rat hepatocyte-derived cell line occurs without DNA fragmentation.

    Science.gov (United States)

    Bulera, S J; Sattler, C A; Gast, W L; Heath, S; Festerling, T A; Pitot, H C

    1998-10-01

    The hepatotoxicant thioacetamide (TH) has classically been used as a model to study hepatic necrosis; however, recent studies have shown that TH can also induce apoptosis. In this report we demonstrate that 2.68+/-0.54% of the albumin-SV40 T-antigen transgenic rat hepatocytes undergo TH-induced apoptosis, a level comparable to other in vivo models of liver apoptosis. In addition, TH could induce apoptosis and necrosis in the L37 albumin-SV40 T-antigen transgenic rat liver-derived cell line. Examination of dying L37 cells treated with 100 mM TH by electron microscopy revealed distinct morphological characteristics that could be attributed to apoptosis. Quantitation of apoptosis by FACS analysis 24 h after treatment with 100 mM TH revealed that 81.3+/-1.6% of the cells were undergoing apoptosis. In contrast, when L37 cells were treated with 250 mM TH, cells exhibited characteristics consistent with necrotic cell death. DNA fragmentation ladders were produced by growth factor withdrawal-induced apoptosis; however, in 100 mM TH-induced apoptosis, DNA fragmentation ladders were not observed. Analysis of endonuclease activity in L37 cells revealed that the enzymes were not inactivated in the presence of 100 mM TH. The data presented in this report indicate that the L37 cell line could be used to study the mechanism of TH-induced apoptosis that was not mediated through a mechanism requiring DNA fragmentation.

  9. Um Estudo dos Portais Corporativos como Instrumento de Externalização do Conhecimento Explícito em UniversidadesA Study of the Corporative Portals as a Tool of Explicit Knowledge Externalization in UniversitiesUn Estudio de las Páginas Web Corporativas como Instrumento de Externalización del Conocimiento Explícito en Universidades

    Directory of Open Access Journals (Sweden)

    GASPAR, Marcos Antonio

    2009-06-01

    students, the support for the courses as well as the spreading of information to the public interested in joining the institution; whereas the analyzed public universities prioritize more specific information, directed to the dissemination of the research developed internally or with institutional objectives.RESUMENLas páginas web corporativas, viables por medio de la Tecnología de la Información y Comunicación, permiten la integración de datos heterogéneos procedentes de sistemas internos de información, que están disponibles para que la comunidad interesada pueda acceder y participar. Pueden transformarse en importante instrumento de evaluación del conocimiento explícito en la organización, pues el intercambio de información es más rápido y seguro, y propician un saludable ambiente de colaboración. En el caso específico de grandes universidades brasileñas, las páginas web corporativas adquieren significación fundamental, porque ofrecen gran variedad y cantidad de informaciones y conocimentos, en función de la multiplicidad de sus actividades. Este estudio tiene por finalidad investigar por medio del análisis de contenido, aspectos importantes del conocimiento explícito expuesto por las universidades analizadas en sus páginas web corporativas. El estudio fue de carácter exploratorio con prospección mediante observación directa de los contenidos existentes en las páginas web corporativas de dos universidades públicas y tres privadas. Se realizó un análisis comparativo de los contenidos existentes en esas páginas web, que puede ser útil para evaluar su uso como factor de optimización del conocimiento explícito generado en la universidad. Como resultado, se pudo constatar la existencia de diferencias importantes, tanto en la composición como en el contenido de las páginas web corporativas de las universidades públicas en comparación con las páginas de las universidades privadas. Las principales diferenciaciones se refieren al tipo de

  10. Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity.

    Science.gov (United States)

    Mead, Emma J; Masterton, Rosalyn J; Feary, Marc; Obrezanova, Olga; Zhang, Lin; Young, Robert; Smales, C Mark

    2015-12-15

    Translation initiation is on the critical pathway for the production of monoclonal antibodies (mAbs) by mammalian cells. Formation of a closed loop structure comprised of mRNA, a number of eukaryotic initiation factors (eIFs) and ribosomal proteins has been proposed to aid re-initiation of translation and therefore increase global translational efficiency. We have determined mRNA and protein levels of the key components of the closed loop, eIFs (eIF3a, eIF3b, eIF3c, eIF3h, eIF3i and eIF4G1), poly(A)-binding protein (PABP) 1 and PABP-interacting protein 1 (PAIP1), across a panel of 30 recombinant mAb-producing GS-CHOK1SV cell lines with a broad range of growth characteristics and production levels of a model recombinant mAb. We have used a multi-level statistical approach to investigate the relationship between key performance indicators (cell growth and recombinant antibody productivity) and the intracellular amounts of target translation initiation factor proteins and the mRNAs encoding them. We show that high-producing cell lines maintain amounts of the translation initiation factors involved in the formation of the closed loop mRNA, maintaining these proteins at appropriate levels to deliver enhanced recombinant protein production. We then utilize knowledge of the amounts of these factors to build predictive models for and use cluster analysis to identify, high-producing cell lines. The present study therefore defines the translation initiation factor amounts that are associated with highly productive recombinant GS-CHOK1SV cell lines that may be targets for screening highly productive cell lines or to engineer new host cell lines with the potential for enhanced recombinant antibody productivity. © 2015 Authors; published by Portland Press Limited.

  11. p53-stabilizing Agent CP-31398 Prevents Growth and Invasion of Urothelial Cancer of the Bladder in Transgenic UPII-SV40T Mice

    Directory of Open Access Journals (Sweden)

    Venkateshwar Madka

    2013-08-01

    Full Text Available The high prevalence of bladder cancer and its recurrence make it an important target for chemoprevention. About half of invasive urothelial tumors have mutations in p53. We determined the chemopreventive efficacy of a p53-stabilizing agent, CP-31398, in a transgenic UPII-SV40T mouse model of bladder transitional cell carcinoma (TCC that strongly resembles human TCC. After genotyping, six-week-old UPII-SV40T mice (n = 30/group were fed control (AIN-76A or experimental diets containing 150 or 300 ppm of CP-31398 for 34 weeks. Progression of bladder cancer growth was monitored by magnetic resonance imaging. At 40 weeks of age, all mice were killed; urinary bladders were collected to determine weights, tumor incidence, and histopathology. There was a significant increase in bladder weights of transgenic versus wild-type mice (male: 140.2 mg vs 27.3 mg, P < .0001; female: 34.2 mg vs 14.8 mg, P < .0001. A significant decrease in the bladder tumor weights (by 68.6–80.2%, P < .0001 in males and by 36.9–55.3%, P < .0001 in females was observed in CP-31398-treated mice. Invasive papillary TCC incidence was 100% in transgenic mice fed control diet. Both male and female mice exposed to CP-31398 showed inhibition of invasive TCC. CP-31398 (300 ppm completely blocked invasion in female mice. Molecular analysis of the bladder tumors showed an increase in apoptosis markers (p53, p21, Bax, and Annexin V with a decrease in vascular endothelial growth factor in transgenic mice fed CP-31398. These results suggest that p53-modulating agents can serve as potential chemopreventive agents for bladder TCC.

  12. Evaluation of the Reference Numerical Parameters of the Monthly Method in ISO 13790 Considering S/V Ratio

    Directory of Open Access Journals (Sweden)

    Hee-Jeong Kwak

    2015-01-01

    Full Text Available Many studies have investigated the accuracy of the numerical parameters in the application of the quasi steady-state calculation method. The aim of this study is to derive the reference numerical parameters of the ISO 13790 monthly method by reflecting the surface-to-volume (S/V ratio and the characteristics of the structures. The calculation process was established, and the parameters necessary to derive the reference numerical parameters were calculated based on the input data prepared for the established calculation processes. The reference numerical parameters were then derived through regression analyses of the calculated parameters and the time constant. The parameters obtained from an apartment building and the parameters of the international standard were both applied to the Passive House Planning Package (PHPP and EnergyPlus programs, and the results were analyzed in order to evaluate the validity of the results. The analysis revealed that the calculation results based on the parameters derived from this study yielded lower error rates than those based on the default parameters in ISO 13790. However, the differences were shown to be negligible in the case of high heat capacity.

  13. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1941-09-01

    Full Text Available Autores Hill, D. K. Revista Lancet. Abreviación Lancet. Tomo 1, páginas 177-178. Fecha 8-2/41. Determinación del volumen sanguíneo en pacientes con shock / Autores, Jewesbury, E. C. O. Artículo, Reactions after transfusion of stored blood. Revista, British Medical Journal. Abreviación Brit. med. J. Tomo 1, páginas 663-5. Fecha 3-5-41. Reacciones después de la transfusión de sangre conservada / Autores, Mackay, M. E. Artículo, The Bacteriostatic effect of desinfectants in human serum and citrated plasma. Revista, British Medical Journal. Abreviación, Brit. med. J. Tomo 1, páginas 737-750. Fecha 17-5-41 / Autores, Dubash, J. Clegg, O., Vaughan, J. Artículo, Changes ocuring in blood stored in different preservatives. Revista, British Medical Journal. Abreviación Brit. med. J. Tomo 2, páginas 482-4. Fecha 12-10-40 / Alteraciones producidas en sangre conservada con distintos preservativos. Autores, Czekalowski, J. W. Artículo, Studies on stored blood. Revista, Endinburgh Medical Journal. Abreviación, Edinb. med. J. Tomo 48, páginas 405-13. Fecha, Junio, 1941 / Estudios sobre sangre conservada. Autores, Harrison, G. A. Picken, L. E. Artículo, Quantitative aspects of transfusion. Revista Lancet. Tomo 1, página 685. Fecha 31-5-41 / Aspectos cuantitativos de la transfusión.

  14. Lo visual como construcción. Desarrollo de un modelo pictórico de representación de la imagen

    OpenAIRE

    De la Villa Liso, Lourdes

    2012-01-01

    903 páginas, bibliografía en páginas 854-895, glosario en páginas 896-903 [En] Along with the development of neuroscience in the last century until today, has arisen the comparison between our brain function and the work of art. More specifically, the neuroscience of vision compares it with painting. From the point of view of our visual sense, what defines our brain function is the visual mechanism. This thesis tries to approach such a mechanism, from the functioning of the artwork. Suc...

  15. Sub-milliSievert (sub-mSv) CT colonography: a prospective comparison of image quality and polyp conspicuity at reduced-dose versus standard-dose imaging

    Energy Technology Data Exchange (ETDEWEB)

    Lubner, Meghan G.; Pooler, B.D.; Kitchin, Douglas R.; Kim, David H.; Munoz del Rio, Alejandro; Pickhardt, Perry J. [University of Wisconsin School of Medicine and Public Health, E3/311 Clinical Sciences Center, Departments of Radiology, Madison, WI (United States); Tang, Jie [University of Wisconsin School of Medicine and Public Health, Medical Physics, Madison, WI (United States); Li, Ke; Chen, Guang-Hong [University of Wisconsin School of Medicine and Public Health, E3/311 Clinical Sciences Center, Departments of Radiology, Madison, WI (United States); University of Wisconsin School of Medicine and Public Health, Medical Physics, Madison, WI (United States)

    2015-07-15

    To prospectively compare reduced-dose (RD) CT colonography (CTC) with standard-dose (SD) imaging using several reconstruction algorithms. Following SD supine CTC, 40 patients (mean age, 57.3 years; 17 M/23 F; mean BMI, 27.2) underwent an additional RD supine examination (targeted dose reduction, 70-90 %). DLP, CTDI{sub vol}, effective dose, and SSDE were compared. Several reconstruction algorithms were applied to RD series. SD-FBP served as reference standard. Objective image noise, subjective image quality and polyp conspicuity were assessed. Mean CTDI{sub vol} and effective dose for RD series was 0.89 mGy (median 0.65) and 0.6 mSv (median 0.44), compared with 3.8 mGy (median 3.1) and 2.8 mSv (median 2.3) for SD series, respectively. Mean dose reduction was 78 %. Mean image noise was significantly reduced on RD-PICCS (24.3 ± 19HU) and RD-MBIR (19 ± 18HU) compared with RD-FBP (90 ± 33), RD-ASIR (72 ± 27) and SD-FBP (47 ± 14 HU). 2D image quality score was higher with RD-PICCS, RD-MBIR, and SD-FBP (2.7 ± 0.4/2.8 ± 0.4/2.9 ± 0.6) compared with RD-FBP (1.5 ± 0.4) and RD-ASIR (1.8 ± 0.44). A similar trend was seen with 3D image quality scores. Polyp conspicuity scores were similar between SD-FBP/RD-PICCS/RD-MBIR (3.5 ± 0.6/3.2 ± 0.8/3.3 ± 0.6). Sub-milliSievert CTC performed with iterative reconstruction techniques demonstrate decreased image quality compared to SD, but improved image quality compared to RD images reconstructed with FBP. (orig.)

  16. Gestualidad y vacilación

    Directory of Open Access Journals (Sweden)

    Mario Jursich Durán

    1987-04-01

    Full Text Available Entre el silencio de un pensamiento... Bernardo Camacho. Editorial Oveja Negra, Bogotá, 1986,69 páginas. Lluvia de vientos. Jaime León Castaño. Editorial La Tecla, Bogotá, 1986, 83 páginas

  17. Aplicación de normas de ergonomia informatica en paginas web en Panama con miras a la creacion de ambientes usables por personas con discapacidad

    OpenAIRE

    de Fuertes, Laila V.; de Clunie, Gisela T

    2012-01-01

    En este trabajo presentamos un estudio, realizado en la República de Panamá, sobre la aplicación de normas de ergonomía informática en el diseño de páginas web y el nivel de aplicación de las regulaciones de ergonomía de software para páginas web en personas con discapacidad. A manera de contribución, introducimos algunos indicadores relativos al desarrollo de páginas web en Panamá, en el contexto del estudio. Un referente importante para el diagnóstico realizado, lo constituyen las Normas IS...

  18. Reactive Aggregate Model Protecting Against Real-Time Threats

    Science.gov (United States)

    2014-09-01

    IPv4 address space. Cisco products such as Auto Secure and Adaptive Security Appliance are effective for Cisco products, but large distributed...protection capability within GINA. GINA has no published history of implementation as an IPS. A. RAMPART DECISION MODEL In order to establish a threat

  19. La responsabilidad social corporativa en los principales grupos de comunicación españoles: incorporación, gestión y análisis de la información a través de sus páginas web

    Directory of Open Access Journals (Sweden)

    Jessica Fernández Vázquez

    2012-10-01

    Full Text Available La responsabilidad social corporativa (RSC se ha convertido en un activo intangible e imprescindible en la gestión empresarial. Las instituciones son conscientes de que se integran en una comunidad con la que deben mantener un diálogo constante y en la que revierten parte de sus beneficios. De esta forma, la RSC se convierte en una herramienta clave de relaciones públicas a la hora de mejorar su reputación y legitimación social así como para transmitir una identidad corporativa determinada. En el caso de los medios de comunicación, la RSC cobra una doble dimensión: no sólo se trata de aplicar medidas responsables en torno al medio ambiente, grupos de interés, instituciones, etc. sino que debe transmitir la información de forma rigurosa, veraz, objetiva y ética. Teniendo en cuenta esto, decidimos estudiar la RSC de los principales grupos de comunicación en España a partir de la información publicada en sus páginas web.

  20. Estabilización de suelos contaminados. El caso de Aznalcóllar

    OpenAIRE

    Cabrera, Francisco

    2005-01-01

    21 páginas, 11 figuras, 4 tablas, 48 referencias.-- El volumen citado consta de 260 páginas.-- Forma parte de la Colección Congresos y Cursos de la Universidad de Burgos.-- Curso de verano celebrado del 18-22 de julio 2005, en Burgos, España.

  1. Progressive paralysis associated with diffuse astrocyte anaplasia in delta 202 mice homozygous for a transgene encoding the SV40 T antigen.

    Science.gov (United States)

    López-Revilla, Rubén; Soto-Zárate, Carlos; Ridaura, Cecilia; Chávez-Dueñas, Lucía; Paul, Dieter

    2004-03-01

    A convenient transgenic astrocytoma model in delta202 mice, homozygous for a construct encoding the early region of the SV40 virus genome, is described. In the offspring of crosses between delta202 mice heterozygous for the transgene nearly 60% were transgenic; one third of these developed progressive paralysis starting in the hindlimbs at approximately 35 days of age and died at 90 +/- 30 days of age. In affected mice proliferating-non-neuronal cells immunostained with antibodies to the GFAP, an astrocyte marker, whose number increased with age were found in the white matter of the brain, cerebellum and spinal cord, and progressive degeneration and necrosis of spinal motoneurons was observed that-may explain the paralysis. The early onset and reproducible time course of the neurological disease suggest that homozygous delta202 mice, whose proliferating astrocytes appear to damage spinal motoneurons, are a useful model to study astrocyte differentiation, function and tumorigenesis.

  2. Visual narratives in a page: the photography and diagramming of the meanings Narrativas visuais na página:a fotografia e a diagramação dos sentidos

    Directory of Open Access Journals (Sweden)

    Hertez Wendel de Camargo

    2008-03-01

    Full Text Available This article is aimed at analyzing the composition of newsmagazine reportage viewed as a creation process and, specifically, determine its intervention in the meanings of photography. The resulting news diagram and visual narratives reflect the editorial concept of the publication meanwhile speeches are enhanced. Photoreporting appeared in Brazil after the years 40, and marked the start of modern photographic journalism. The piece of a photoreporting entitled 4 a 1 published by O Cruzeiro magazine is intended to be analyzed here. A photoreporting covering a mere soccer match between a São Paulo and a Rio de Janeiro team discloses how diagramming seeks to reset news timing and how the meanings in Jean Manzon images are extended beyond the pages. Este artigo objetiva analisar a composição da reportagem em revista como processo de criação e, especificamente, a sua intervenção nos sentidos da fotografia. A diagramação da notícia e as narrativas visuais resultantes refletem o conceito editorial da publicação ao mesmo tempo em que potencializam discursos. A fotorreportagem surge no Brasil a partir dos anos 40, marcando o início do fotojornalismo moderno. Pretende-se analisar a fotorreportagem 4 a 1, publicada em março de 1947, na revista O Cruzeiro. A matéria sobre uma simples partida de futebol entre times paulista e carioca revela como a diagramação busca a recomposição do tempo da notícia e de como os sentidos das imagens de Jean Manzon são estendidos além das páginas.

  3. Differential effects of simple repeating DNA sequences on gene expression from the SV40 early promoter.

    Science.gov (United States)

    Amirhaeri, S; Wohlrab, F; Wells, R D

    1995-02-17

    The influence of simple repeat sequences, cloned into different positions relative to the SV40 early promoter/enhancer, on the transient expression of the chloramphenicol acetyltransferase (CAT) gene was investigated. Insertion of (G)29.(C)29 in either orientation into the 5'-untranslated region of the CAT gene reduced expression in CV-1 cells 50-100 fold when compared with controls with random sequence inserts. Analysis of CAT-specific mRNA levels demonstrated that the effect was due to a reduction of CAT mRNA production rather than to posttranscriptional events. In contrast, insertion of the same insert in either orientation upstream of the promoter-enhancer or downstream of the gene stimulated gene expression 2-3-fold. These effects could be reversed by cotransfection of a competitor plasmid carrying (G)25.(C)25 sequences. The results suggest that a G.C-binding transcription factor modulates gene expression in this system and that promoter strength can be regulated by providing protein-binding sites in trans. Although constructs containing longer tracts of alternating (C-G), (T-G), or (A-T) sequences inhibited CAT expression when inserted in the 5'-untranslated region of the CAT gene, the amount of CAT mRNA was unaffected. Hence, these inhibitions must be due to posttranscriptional events, presumably at the level of translation. These effects of microsatellite sequences on gene expression are discussed with respect to recent data on related simple repeat sequences which cause several human genetic diseases.

  4. On the risk to low doses (<100 mSv) of ionizing radiation during medical imaging procedures - IOMP policy statement

    International Nuclear Information System (INIS)

    Pradhan, A.S.

    2013-01-01

    The science committee of International Organization for Medical Physics (IOMP) developed a policy statement on the predictions of radiation-induced cancers and cancer deaths in patients exposed to low doses (<100 mSv) of ionizing radiation during medical imaging; this statement has been approved by the IOMP council. In order to attract the attention of medical physicists, an editorial (1) titled 'Risk of Medical Imaging' that includes the said statement has recently been published in Medical Physics journal of American Association of Physicists in Medicine (AAPM). As stated, IOMP represents 80 national and 6 regional medical physics organizations and 18,000 medical physicists worldwide. The IOMP affiliated bodies/organizations in different countries (such as Association of Medical Physicists of India, AMPI) have been encouraged to reproduce the IOMP statement in their journals/newsletters for the benefit of larger community of medical physicists. The IOMP statement is reproduced below (readers may also go through the supportive literature listed in references). It is hoped that this policy statement will have some deterrent influence on the continued propagation of unproven risk related to medical imaging procedures conducted with small doses.

  5. Suitability of digital elevation models generated by uav photogrammetry for slope stability assessment (case study of landslide in Svätý Anton, Slovakia

    Directory of Open Access Journals (Sweden)

    Rusnák Miloš

    2016-12-01

    Full Text Available Assessing the accuracy of photogrammetrically-derived digital elevation models (DEMs from UAV is essential in many geoscience disciplines. The suitability of different DEM devised for slope stability assessment was evaluated in the example of the landslide in Svätý Anton village in Slovakia. Aerial data was acquired during a one-day field campaign in autumn 2014. The point cloud from 218 images (54,607,748 points was manually classified into 7 different classes for filtering vegetation cover and buildings. Assessment of vertical differences between the UAV derived elevation model and real terrain surface was based on comparison of control points targeted by GPS (337 points and unclassified and ground classified point cloud for raster elevation models with 1, 5, 10, 20 and 50 cm pixel resolution.

  6. Improving Weak Lensing Mass Map Reconstructions using Gaussian and Sparsity Priors: Application to DES SV

    Science.gov (United States)

    Jeffrey, N.; Abdalla, F. B.; Lahav, O.; Lanusse, F.; Starck, J.-L.; Leonard, A.; Kirk, D.; Chang, C.; Baxter, E.; Kacprzak, T.; Seitz, S.; Vikram, V.; Whiteway, L.; Abbott, T. M. C.; Allam, S.; Avila, S.; Bertin, E.; Brooks, D.; Rosell, A. Carnero; Kind, M. Carrasco; Carretero, J.; Castander, F. J.; Crocce, M.; Cunha, C. E.; D'Andrea, C. B.; da Costa, L. N.; Davis, C.; De Vicente, J.; Desai, S.; Doel, P.; Eifler, T. F.; Evrard, A. E.; Flaugher, B.; Fosalba, P.; Frieman, J.; García-Bellido, J.; Gerdes, D. W.; Gruen, D.; Gruendl, R. A.; Gschwend, J.; Gutierrez, G.; Hartley, W. G.; Honscheid, K.; Hoyle, B.; James, D. J.; Jarvis, M.; Kuehn, K.; Lima, M.; Lin, H.; March, M.; Melchior, P.; Menanteau, F.; Miquel, R.; Plazas, A. A.; Reil, K.; Roodman, A.; Sanchez, E.; Scarpine, V.; Schubnell, M.; Sevilla-Noarbe, I.; Smith, M.; Soares-Santos, M.; Sobreira, F.; Suchyta, E.; Swanson, M. E. C.; Tarle, G.; Thomas, D.; Walker, A. R.

    2018-05-01

    Mapping the underlying density field, including non-visible dark matter, using weak gravitational lensing measurements is now a standard tool in cosmology. Due to its importance to the science results of current and upcoming surveys, the quality of the convergence reconstruction methods should be well understood. We compare three methods: Kaiser-Squires (KS), Wiener filter, and GLIMPSE. KS is a direct inversion, not accounting for survey masks or noise. The Wiener filter is well-motivated for Gaussian density fields in a Bayesian framework. GLIMPSE uses sparsity, aiming to reconstruct non-linearities in the density field. We compare these methods with several tests using public Dark Energy Survey (DES) Science Verification (SV) data and realistic DES simulations. The Wiener filter and GLIMPSE offer substantial improvements over smoothed KS with a range of metrics. Both the Wiener filter and GLIMPSE convergence reconstructions show a 12% improvement in Pearson correlation with the underlying truth from simulations. To compare the mapping methods' abilities to find mass peaks, we measure the difference between peak counts from simulated ΛCDM shear catalogues and catalogues with no mass fluctuations (a standard data vector when inferring cosmology from peak statistics); the maximum signal-to-noise of these peak statistics is increased by a factor of 3.5 for the Wiener filter and 9 for GLIMPSE. With simulations we measure the reconstruction of the harmonic phases; the phase residuals' concentration is improved 17% by GLIMPSE and 18% by the Wiener filter. The correlation between reconstructions from data and foreground redMaPPer clusters is increased 18% by the Wiener filter and 32% by GLIMPSE.

  7. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1941-12-01

    Full Text Available Autores, Patey, D. H., Robertson, J. D. Artículo, comprenssion treatment of crush injuries of limbs. Revista, Lancet. Tomo 1. Páginas 780-782. Tratamiento por comprensión de las lesiones de los miembros a consecuencia de aplastamiento / Autores. Della Vida, B. L. Dyke, S. c. Arículo, blood-Picture in trichiniasis. Revista, Lancet. Abreviación, Tomo 2. Páginas 67-71. Fecha 19/7/41. Cuadro hemático en la triquinosis. Autores Bowesman, C. Artículo, A. Short report on the use of 4:4' - Diamidi - no stilbene in the treatment of human sleeping sickness. Revista, Annals of Tropical Medicine and Parasitology. Abreviación, Ann. Trop. Med. & Parasit. Tomo 34. páginas 217-222. Fecha 31/12/40 . Informe breve sobre el empleo del 4:4' - diamidino stilbeno en el tratamiento de la enfermedad del sueño en el hombre. Autores, Findlay, G. M. Artículo, The action of sulphanilamide on the virus of lymphogranuloma venereum. Revista, Britis Journal of Experimental Pathology. Abreviación, Brit. J. Exper. Path. Tomo 21. Páginas, 356-360. Fecha, diciembre, 1940. La acción de la sulfanilamida sobre el virus del linfogranuloma venereo. Autores, Beck, S. Peacock, P. R. Artículo Gastro-Papillomatosis due to Vitamin A deficiency induced by heated fats. Revista, British Medical Journal. Abreviación. Brit. med. J. Tomo 2. Páginas 81-83. Fecha 19/7/41 / Gastro-papilomatosis debida a deficiencia en vitamina a provocada por calentamiento de grasas. Autores, Sommerville, J. Artículo, Acute Gonorrhoea: Further Observation on treatment by sulphapiridine fowolled by Lavage. Revista, British Medical Journal. Abreviación, Brit. med. J. Tomo 1. Páginas 961-962-28-6/41. Gonorrea aguda: nuevas observaciones sobre el tratamiento con sulfapiridina seguido de lavados.

  8. Novedades Bibliográficas

    Directory of Open Access Journals (Sweden)

    anurio Colombiano de Historia social y de la cultura

    2003-01-01

    Full Text Available Jaime Humberto Borja, Los indios medievales de Fray Pedro de Aguado. Construcción del idólatra y escritura de la historia en una crónica del siglo XVI. Bogotá: Pontificia Universidad Javeriana - Icanh, 2002, 247 páginas. / Testamentos Indígenas de Santafé de Bogotá, siglos XVI-XVII. Edición y prólogo de Pablo Rodríguez Jiménez. Bogotá: Instituto Distrital de Cultura y turismo - Alcaldía Mayor de Bogotá D.C., 2002. /  Juan Carlos Pérgolis, La Plaza: el centro de la ciudad. Bogotá: Universidad Católica de Colombia- Universidad Nacional de Colombia, 223 páginas. / Clément Thibaud, Repúblicas en armas. Los ejércitos bolivarianos en la guerra de Independencia en Colombia y Venezuela. Bogotá: Editorial Planeta, 2003. 571 páginas. / Proceso de beatificación y canonización de San Pedro Claver. Anna María Splenadianni y Tulio Aristizábal, S.J. (editores. Bogotá: Pontificia Universidad Javeriana - Universidad Católica del Táchira, 2002, 593 páginas. / La Terapéutica en el Nuevo Reino de Granada: un recetario franciscano del siglo XVIII. Editado por el Dr. Santiago Díaz y el padre Luis Carlos Mantilla. Bogotá: Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 2002, 208 páginas. / Cartagena de Indias en el Siglo XIX. Haroldo Calvo y Adolfo Meisel (Editores. Cartagena: Universidad Jorge Tadeo Lozano - Banco de la República, 2002. /  Osear Almario García, Los Renacientes y su territorio: ensayos sobre la etnicidad negra en el Pacífico su colombiano. Medellín: Concejo de Medellín - Universidad pontificia Bolivariana, 2003, 276 páginas. /  Ricardo Sánchez, De la memoria a la acción: crítica histórica. Cali: Universidad del Valle, 2003, 248 páginas. / Santiago Montenegro. El arduo tránsito hacia la modernidad. Historia de la industria textil durante la primera mitad del siglo xx: Medellín: Editorial Universidad de Antioquia - Facultad de Ciencias Humanas y Económicas - Universidad Nacional de

  9. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1943-09-01

    Full Text Available British Medical Information Service. - 3 Hanover Street London, W 1. Autores Klaber, r. Revista British Journal of Dermatology and Syphilis. Abreviación Brit. J. Derm. Syph. Tomo 54. Páginas 193-211. Fecha julio 1942 / Fito-foto-dermatitis. Autores Taylor H. M. Revista Journal of Obstetrics & Gynaecology of the British Empire. Abreviación j. Obste. Gynaec. Brit. Emp. Tomo 49. Páginas 341-367. Fecha, Agosto, 1942 / Un estudio clínico de la mestruación con referencia especial a la dismenorrea primaria. Autores: Alcock, H. B. Revista, The Medical Press and Circular. Abreviación, Med. Press & Circular. Páginas 44-46. Fecha 15/7/42 / Anestesia en pacientes ancianos.

  10. SvSXP

    DEFF Research Database (Denmark)

    Andersen, Ulla Vestergaard; Howe, Daniel K.; Dangoudoubiyam, Sriveny

    2013-01-01

    Strongyle parasites are ubiquitous in grazing horses. Strongylus vulgaris, the most pathogenic of the large strongyles, is known for its extensive migration in the mesenteric arterial system. The lifecycle of S. vulgaris is characterised by a long prepatent period where the migrating larvae...... are virtually undetectable as there currently is no test available for diagnosing prepatent S. vulgaris infection. Presence of S. vulgaris larvae in the arterial system causes endarteritis and thrombosis with a risk of non-strangulating intestinal infarctions. Emergence of anthelmintic resistance among...... cyathostomins has led to recommendations of reduced treatment intensity by targeting horses that exceed a predetermined strongyle faecal egg count threshold. One study suggests an apparent increase in prevalence of S. vulgaris on farms where reduced anthelmintic treatment intensity has been implemented...

  11. SV-AUTOPILOT

    NARCIS (Netherlands)

    Leung, Wai Yi; Marschall, Tobias; Paudel, Yogesh; Falquet, Laurent; Mei, Hailiang; Schönhuth, Alexander; Maoz Moss, Tiffanie Yael

    2015-01-01

    Background: Many tools exist to predict structural variants (SVs), utilizing a variety of algorithms. However, they have largely been developed and tested on human germline or somatic (e.g. cancer) variation. It seems appropriate to exploit this wealth of technology available for humans also for

  12. Rat bone marrow progenitor cells transduced in situ by rSV40 vectors differentiate into multiple central nervous system cell lineages.

    Science.gov (United States)

    Louboutin, Jean-Pierre; Liu, Bianling; Reyes, Beverly A S; Van Bockstaele, Elisabeth J; Strayer, David S

    2006-12-01

    Using bone marrow-directed gene transfer, we tested whether bone marrow-derived cells may function as progenitors of central nervous system (CNS) cells in adult animals. SV40-derived gene delivery vectors were injected directly into femoral bone marrow, and we examined transgene expression in blood and brain for 0-16 months thereafter by immunostaining for FLAG epitope marker. An average of 5% of peripheral blood cells and 25% of femoral marrow cells were FLAG(+) throughout the study. CNS FLAG-expressing cells were mainly detected in the dentate gyrus (DG) and periventricular subependymal zone (PSZ). Although absent before 1 month and rare at 4 months, DG and PSZ FLAG(+) cells were abundant 16 months after bone marrow injection. Approximately 5% of DG cells expressed FLAG, including neurons (48.6%) and microglia (49.7%), and occasional astrocytes (1.6%), as determined by double immunostaining for FLAG and lineage markers. These data suggest that one or more populations of cells resident within adult bone marrow can migrate to the brain and differentiate into CNS-specific cells.

  13. Improving Weak Lensing Mass Map Reconstructions using Gaussian and Sparsity Priors: Application to DES SV

    Energy Technology Data Exchange (ETDEWEB)

    Jeffrey, N.; et al.

    2018-01-26

    Mapping the underlying density field, including non-visible dark matter, using weak gravitational lensing measurements is now a standard tool in cosmology. Due to its importance to the science results of current and upcoming surveys, the quality of the convergence reconstruction methods should be well understood. We compare three different mass map reconstruction methods: Kaiser-Squires (KS), Wiener filter, and GLIMPSE. KS is a direct inversion method, taking no account of survey masks or noise. The Wiener filter is well motivated for Gaussian density fields in a Bayesian framework. The GLIMPSE method uses sparsity, with the aim of reconstructing non-linearities in the density field. We compare these methods with a series of tests on the public Dark Energy Survey (DES) Science Verification (SV) data and on realistic DES simulations. The Wiener filter and GLIMPSE methods offer substantial improvement on the standard smoothed KS with a range of metrics. For both the Wiener filter and GLIMPSE convergence reconstructions we present a 12% improvement in Pearson correlation with the underlying truth from simulations. To compare the mapping methods' abilities to find mass peaks, we measure the difference between peak counts from simulated {\\Lambda}CDM shear catalogues and catalogues with no mass fluctuations. This is a standard data vector when inferring cosmology from peak statistics. The maximum signal-to-noise value of these peak statistic data vectors was increased by a factor of 3.5 for the Wiener filter and by a factor of 9 using GLIMPSE. With simulations we measure the reconstruction of the harmonic phases, showing that the concentration of the phase residuals is improved 17% by GLIMPSE and 18% by the Wiener filter. We show that the correlation between the reconstructions from data and the foreground redMaPPer clusters is increased 18% by the Wiener filter and 32% by GLIMPSE. [Abridged

  14. Discursos para o feminino em páginas da revista Querida (1958-1968: aproximações Women discourses in pages of Querida magazine (1958-1968: approaches

    Directory of Open Access Journals (Sweden)

    Laura Peretto Salerno

    2011-06-01

    Full Text Available A imprensa feminina desempenha importante papel na educação de mulheres letradas. Nas décadas de 50 e 60 do século XX, as revistas femininas ocupavam um lugar de destaque na vida de suas leitoras, dialogando com elas sobre problemas cotidianos. Eram conselheiras e confidentes; companheiras de lazer. A partir disso é possível afirmar que tais periódicos podem colaborar para a manutenção de determinados padrões, veiculando papéis ditos tradicionais de mulher, de comportamento, de sexualidade e de relações de gênero. As fontes da pesquisa são compostas por 31 volumes da Revista Querida com exemplares que representam os anos compreendidos entre 1958 e 1968. O objetivo da pesquisa com tais revistas seria procurar compreender que modos de comportamento os discursos presentes na coluna Certo e Errado de Querida (1958-1968 teriam contribuído para sugerir e que representações de uma época esses discursos, veiculados nas páginas das revistas selecionadas, ecoaram.Women's press has an important role in the education of literate women. In the 50's and 60's of the twentieth century women's magazines occupied a prominent place in the lives of its readers, talking to them about everyday problems. They were counselors and confidants, entertainment partners. From this it can be said that such journals may contribute to the maintenance of certain standards, passing so-called traditional roles of women, behavior, sexuality and gender relations. The research sources are composed of 31 volumes of the Querida magazine with copies representing the years between 1958 and 1968. The objective of this research with such magazines is to seek to understand patterns of behavior that the speeches in the column Right and Wrong in Querida (1958-1968 would have contributed to suggest and what representations of a time these speeches, which appeared on pages of the selected magazines, echoed.

  15. Limits of transforming competence of SV40 nuclear and cytoplasmic large T mutants with altered Rb binding sequences.

    Science.gov (United States)

    Tedesco, D; Fischer-Fantuzzi, L; Vesco, C

    1993-03-01

    Multiple amino acid substitutions were introduced into the SV40 large T region that harbors the retinoblastoma protein (Rb) binding site and the nuclear transport signal, changing either one or both of these determinants. Mutant activities were examined in a set of assays allowing different levels of transforming potential to be distinguished; phenotypic changes in established and pre-crisis rat embryo fibroblasts (REFs) were detected under isogenic cell conditions, and comparisons made with other established rodent cells. The limit of the transforming ability of mutants with important substitutions in the Rb binding site fell between two transformation levels of the same established rat cells. Such cells could be induced to form dense foci but not agar colonies (their parental pre-crises REFs, as expected, were untransformed either way). Nonetheless, agar colony induction was possible in other cell lines, such as mouse NIH3T3 and (for one of the mutants) rat F2408. All these mutants efficiently immortalized pre-crisis REFs. The transforming ability of cytoplasmic mutants appeared to depend on the integrity of the Rb-binding sequence to approximately the same extent as that of the wild-type large T, although evidence of in vivo Rb-cytoplasmic large T complexes was not found. The presence or absence of small t was critical when the transforming task of mutants was near the limit of their abilities.

  16. Influencia del fuego en la evolución de un suelo arenoso

    OpenAIRE

    Siljeström, Patricia; Clemente Salas, Luis

    1987-01-01

    14 páginas, 2 tablas, 2 perfiles, 13 referencias. Este trabajo se presentó como Comunicación a la VII Reunión sobre el Cuaternario de la Asociación Española para el Estudio del Cuaternario, celebrada en Santander, en 1987. Páginas 73-76, Capítulo II: Edafología, del libro de Actas.

  17. Generacion de Nuevas Capacidades en el Ejercito de Guatemala para el Combate al Trafico de Drogas y Delitos Conexos (Generating New Capacities within the Guatemalan Army in Order to Combat Drug Trafficking and Related Crimes)

    Science.gov (United States)

    2014-06-13

    parcial de los requisitos para la obtención del grado de MAESTRÍA EN CIENCIAS Y ARTES MILITARES Estudios Generales Por MARIO ANTONIO CASTILLO...MAESTRÍA EN ARTES Y CIENCIAS MILITARES PÁGINA DE APROBACIÓN DE LA TESIS Nombre del Candidato: Mayor Mario Antonio Castillo Paredes Título...TABLA DE CONTENIDO Página MAESTRÍA EN ARTES Y CIENCIAS

  18. Información, autoayuda y creación de identidades: las tecnologías de la información y la comunicación (TIC y las asociaciones de discapacitados físicos. El ejemplo de la poliomielitis

    Directory of Open Access Journals (Sweden)

    Rosa Ballester

    2011-01-01

    criterios de credibilidad (r de Pearson = 0,81, p<0,001, por lo que se sugiere el Indicador de Credibilidad como una herramienta útil para determinar la calidad de las páginas web. Su uso debe complementarse con otro tipo de acercamientos cualitativos que exploren el contexto de las relaciones expertos-legos y las funciones que dichas páginas cumplen.

  19. Branching ratios, CP asymmetries and polarizations of B → ψ(2S)V decays

    Energy Technology Data Exchange (ETDEWEB)

    Rui, Zhou [North China University of Science and Technology, College of Sciences, Tangshan (China); Li, Ya; Xiao, Zhen-Jun [Nanjing Normal University, Department of Physics and Institute of Theoretical Physics, Nanjing, Jiangsu (China)

    2017-09-15

    We analyze the non-leptonic decays B/B{sub s} → ψ(2S)V with V = (ρ, ω, K*, φ) by employing the perturbative QCD (pQCD) factorization approach. Here the branching ratios, the CP asymmetries and the complete set of polarization observables are investigated systematically. Besides the traditional contributions from the factorizable and non-factorizable diagrams at the leading order, the next-to-leading order (NLO) vertex corrections could also provide considerable contributions. The pQCD predictions for the branching ratios of the B{sub (s)} → ψ(2S)K*, ψ(2S)φ decays are consistent with the measured values within errors. As for B → ψ(2S)ρ, ψ(2S)ω decays, the branching ratios can reach the order of 10{sup -5} and could be measured in the LHCb and Belle-II experiments. The numerical results show that the direct CP asymmetries of the considered decays are very small. Thus the observation of any large direct CP asymmetry for these decays will be a signal for new physics. The mixing-induced CP asymmetries in the neutral modes are very close to sin 2β{sub (s)}, which suggests that these channels can give a cross-check on the measurement of the Cabbibo-Kobayashi-Maskawa (CKM) angle β and β{sub s}. We find that the longitudinal polarization fractions f{sub 0} are suppressed to ∝ 50% due to the large non-factorizable contributions. The magnitudes and phases of the two transverse amplitudes A {sub parallel} and A {sub perpendicular} {sub to} are roughly equal, which is an indication for the approximate light-quark helicity conservation in these decays. The overall polarization observables of B → ψ(2S)K{sup *0} and B{sub s} → ψ(2S)φ channels are also in good agreement with the experimental measurements as reported by LHCb and BaBar. Other results can also be tested by the LHCb and Belle-II experiments. (orig.)

  20. Sobre neología y contextos de uso: Análisis pragmalingüístico de lo ecológico y de lo sostenible en normativas y páginas web de promoción turística

    Directory of Open Access Journals (Sweden)

    Julia Sanmartín Saez

    2016-04-01

    Full Text Available Esta investigación pretende averiguar si existe alguna correlación entre los neologismos que conllevan un valor de “protección del medio ambiente” y se crean con el formante eco- o mediante composición sintagmática con sustentable, sostenible y ecológico, y dos parámetros: el primer parámetro se corresponde con la variación geolectal del español en España y en América; y el segundo parámetro, con la inserción de estos neologismos en tres tipos de géneros discursivos del turismo (páginas web comerciales, institucionales y textos legales. Para ello, se revisa un corpus de géneros del sector turístico (COMETVAL con el programa de cuantificación léxica AntConc 3.4.1 (Anthony, 2014 y se contrasta con la nomenclatura de los diccionarios generales a modo de filtro neológico. El análisis permite constatar el predominio de voces con valor de “protección” en América, la implicación entre proceso lexicogenético activado y tipo de género discursivo, y la mayor capacidad de eco- para lexicalizar las nuevas formaciones

  1. 13C and 15N CP/MAS, 1H-15N SCT CP/MAS and FTIR spectroscopy as tools for qualitative detection of the presence of zwitterionic and non-ionic forms of ansa-macrolide 3-formylrifamycin SV and its derivatives in solid state.

    Science.gov (United States)

    Przybylski, Piotr; Pyta, Krystian; Klich, Katarzyna; Schilf, Wojciech; Kamieński, Bohdan

    2014-01-01

    (13)C, (15)N CP/MAS, including (1)H-(13)C and (1)H-(15)N short contact time CP/MAS experiments, and FTIR methods were applied for detailed structural characterization of ansa-macrolides as 3-formylrifamycin SV (1) and its derivatives (2-6) in crystal and in powder forms. Although HPLC chromatograms for 2/CH3 OH and 2/CH3 CCl3 were the same for rifampicin crystals dissolved in respective solvents, the UV-vis data recorded for them were different in 300-375 nm region. Detailed solid state (13)C and (15)N CP/MAS NMR and FTIR studies revealed that rifampicin (2), in contrast to 3-formylrifamycin SV (1) and its amino derivatives (3-6), can occur in pure non-ionic or zwitterionic forms in crystal and in pure these forms or a mixture of them in a powder. Multinuclear CP/MAS and FTIR studies demonstrated also that 3-6 derivatives were present exclusively in pure zwitterionic forms, both in powder and in crystal. On the basis of the solid state NMR and FTIR studies, two conformers of 3-formylrifamycin SV were detected in powder form due to the different orientations of carbonyl group of amide moiety. The PM6 molecular modeling at the semi-empirical level of theory, allowed visualization the most energetically favorable non-ionic and zwitterionic forms of 1-6 antibiotics, strongly stabilized via intramolecular H-bonds. FTIR studies indicated that the originally adopted forms of these type antibiotics in crystal or in powder are stable in standard laboratory conditions in time. The results presented point to the fact that because of a possible presence of two forms of rifampicin (compound 2), quantification of the content of this antibiotic in relevant pharmaceuticals needs caution. Copyright © 2013 John Wiley & Sons, Ltd.

  2. Follow-up study of the health experience of U.S. Department of Energy (DOE) workers exposed to >=50mSv (5 rems) per year

    International Nuclear Information System (INIS)

    Fry, S.A.; Hudson, D.R.H.; Robie, D.M.; Lushbaugh, C.C.; Rudnick, S.A.; Shy, C.M.

    1982-01-01

    In 1978, the U.S. Department of Energy initiated a study of its employees reported to have equalled or exceeded the current occupational limit of 50 mSv per year since 1947. The 3200 employees under study were involved in the post-mining and pre-waste disposal phases of the nuclear fuel cycle. The objective of the study is to identify any excess mortality or morbidity in these employees and to determine whether or not any such increase is attributable to occupational exposure to radiation. Standardized mortality ratios will be developed for all and specific causes by comparing the age and sex adjusted mortality rates in the cohort initially with similarly adjusted rates in the general U.S. population. Exploratory analysis of these parameters, based on currently available data, has shown deficits in the cohort of deaths due to all causes, all malignant neoplasms and diseases of the circulatory system. (U.K.)

  3. Canalització de l'oferta i la demanda d'esdeveniments

    OpenAIRE

    Ricart Balart, Albert

    2015-01-01

    Aquest projecte pretén mostrar l'estudi previ realitzat per crear una pàgina web dedicada a la canalització de l'oferta i la demanda d'esdeveniments. Este proyecto pretende mostrar el estudio previo realizado para crear una página web dedicada a la canalización de la oferta y la demanda de eventos. Bachelor thesis for the Computer Science program on Human-computer interaction.

  4. Physiotherapy in asthma--seeking consensus.

    Science.gov (United States)

    Nowobilski, Roman; Plaszewski, Maciej; Wloch, Tomasz; Mika, Piotr; Gajewski, Piotr; Brożek, Jan L

    2013-08-01

    The evidence base for or against physiotherapy interventions in asthmatic adults remains ambiguous, and there are discrepancies between different clinical practice guidelines. We evaluated the level of agreement between the recommendations about physiotherapy for adults with asthma in two major clinical practice guidelines: the Global Initiative for Asthma (GINA 2011) and the British Thoracic Society and the Association of Chartered Physiotherapists in Respiratory Care (BTS/ACPRC 2009). We used the Appraisal of Guidelines for Research & Evaluation (AGREE II) instrument to assess the methodological rigor of the guideline development, the Assessment of Multiple Systematic Reviews (AMSTAR) tool and the Physiotherapy Evidence Database (PEDro) scale to assess the methodological quality of systematic reviews and clinical trials included in the analyzed documents. Additionally, we compared the reference lists of the analyzed sections to establish the overlap in included primary and secondary studies. We observed no agreement between the two guidelines in the choice of source research articles. Only two studies out of 18 used in BTS guidelines were used in the GINA. The reason why GINA developers did not use the body of evidence included in BTS is that it is not clear. Three independent investigators indicated higher scores in all domains of the AGREE II in the BTS/ACPRC document in comparison with the GINA guidelines. The significant differences in the content and in the development processes of the examined sections of the two guidelines suggest the need for more frequent and careful updating or directing the readers of the GINA to the BTS/ACPRC, a guideline addressing specifically and more comprehensively physiotherapy interventions in asthma.

  5. Revista de revistas

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1943-12-01

    Full Text Available Autores, Sandison, R. A., McGregor, J. S. Revista, Journal od Mental Science. Abreviación, J. ment. Sci. Tomo 88. páginas, 387-406. Fecha, julio, 1942. Una experiencia de un año de insulina intravenosa en el tratamiento de la esquizofrenia mediante shock higlucemico / Autores Botterell, E. H., Jefferson, G. Revista, British Medical Journal. Abreviación, Brit. med. J. Tomo 1. Paginas 781-783. Fecha, 27-6-42 / Tratamiento de las heridas de cuero cabelludo en bajas por raid aéreo y otros accidentes / Autores, Clegg, J. W., King, E. J. Revista, British Medical Journal. Abreviación, Brit. med. J. Tomo 2. Páginas 329-333. Fecha 19-9-42 / Determinación de la hemoglobina por el método de la hematina alcalina. Autores, Henry, T. C. Revista, British Medical Journal. Abreviación. Brit. med. J. Tomo 2. Páginas 273-276. Fecha, 5-9-42 / Estomatitis úlcero-membranosa. Autores, Campbell, J. A. Revista, British journal of Experimental Pathology. Abreviación, Brit. J. exp. Path. Tomo 23. Páginas 191-201, Fecha, agosto 1942 / La incidencia de tumores primarios de pulmón en una cepa mixta de ratones.

  6. Scalable Biomarker Discovery for Diverse High-Dimensional Phenotypes

    Science.gov (United States)

    2015-11-23

    William D. Shannon, Richard R. Sharp, Thomas J. Sharpton, Narmada Shenoy, Nihar U. Sheth, Gina A. Simone, Indresh Singh, Chris S. Smillie, Jack D... William D. Shannon, Richard R. Sharp, Thomas J. Sharpton, Narmada Shenoy, Nihar U. Sheth, Gina A. Simone, Indresh Singh, Christopher S. Smillie, Jack D...Susanne J. Szabo, Jeff Porter, Harri Lähdesmäki, Curtis Huttenhower, Dirk Gevers, Thomas W. Cullen , Mikael Knip, on behalf of the DIABIMMUNE Study Group

  7. Synthesis of Pd₃Co₁@Pt/C core-shell catalysts for methanol-tolerant cathodes of direct methanol fuel cells.

    Science.gov (United States)

    Aricò, Antonino S; Stassi, Alessandro; D'Urso, Claudia; Sebastián, David; Baglio, Vincenzo

    2014-08-18

    A composite Pd-based electrocatalyst consisting of a surface layer of Pt (5 wt.%) supported on a core Pd3Co1 alloy (95 wt.%) and dispersed as nanoparticles on a carbon black support (50 wt.% metal content) was prepared by using a sulphite-complex route. The structure, composition, morphology, and surface properties of the catalyst were investigated by XRD, XRF, TEM, XPS and low-energy ion scattering spectroscopy (LE-ISS). The catalyst showed an enrichment of Pt on the surface and a smaller content of Co in the outermost layers. These characteristics allow a decrease the Pt content in direct methanol fuel cell cathode electrodes (from 1 to 0.06 mg cm(-2)) without significant decay in performance, due also to a better tolerance to methanol permeated through the polymer electrolyte membrane. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Review of the thesis: Remnyova S.V. Crime prevention in Leningrad and the Leningrad Region between the later 1950s and the first half of the 1960s

    Directory of Open Access Journals (Sweden)

    Frolov Vasiliy Vladimirovich

    2017-10-01

    Full Text Available This article analyses the thesis “Crime prevention in Leningrad and the Leningrad Region between the later 1950s and the first half of the 1960s” (St. Petersburg, 2016. 254 p. for a Candidate Degree in History by S.V. Remneva as well as the structure and logic of work, the validity of the conclusions, the merits of research and its controversial points. Special attention was paid to the analysis of interaction between the public and law enforcement agencies in crime fighting in the second half of the 1950s and the first half of the 1960s. In conclusion the reviewer pays attention on the idea that the presented facts, assessments and results can be used to develop textbooks on the history of crime, the history of law enforcement community, the history of Leningrad and the Leningrad Region.

  9. Analysis of proviral integration in human mammary epithelial cell lines immortalized by retroviral infection with a temperature-sensitive SV40 T-antigen construct.

    Science.gov (United States)

    Stamps, A C; Davies, S C; Burman, J; O'Hare, M J

    1994-06-15

    A panel of eight conditionally immortal lines derived by infection of human breast epithelial cells with an amphotropic retrovirus transducing a ts mutant of SV40 large T-antigen was analyzed with respect to individual retroviral integration patterns. Each line contained multiple integration sites which were clonal and stable over extended passage. Similar integration patterns were observed between individual lines arising separately from the same stock of pre-immortal cells, suggesting a common progenitor. Retroviral integration analysis of pre-immortal cells at different stages of pre-crisis growth showed changes indicative of a progressive transition from polyclonality to clonality as the cells approached crisis. Each of the immortal lines contained a sub-set of the integration sites of their pre-immortal progenitors, with individual combinations and copy numbers of sites. Since all the cell lines appeared to originate from single foci in separate flasks, it is likely that each set arose from a common clone of pre-immortal cells as the result of separate genetic events. There was no evidence from this analysis to suggest that specific integration sites played any part either in the selection of pre-crisis clones or in the subsequent establishment of immortal lines.

  10. ginas de colectivos LGBT portugueses

    Directory of Open Access Journals (Sweden)

    Ana Cristina Santos

    2012-10-01

    Full Text Available Associação Cultural Janela Indiscreta: http://www.lisbonfilmfest.org/ AT – Associação para o Estudo e Defesa dos Direitos à Identidade de Género: http://a-trans.planetaclix.pt/ Clube Safo: http://www.clubesafo.com/ ILGA Portugal: http://ilga-portugal.oninet.pt/ não te prives – Grupo de Defesa dos Direitos Sexuais: http://www.naoteprives.org/ Opus Gay: http://www.opusgay.org/ Panteras Rosa – Frente de Combate à LesBiGayTransfobia: http://www.panterasrosa.com/ Ponto Bi: http://www.pontobi....

  11. ¿Cómo se dice me he muerto en inglés? Memory. Una webquest sobre el humor en la clase de ELE

    Directory of Open Access Journals (Sweden)

    Adrián Cabedo Nebot

    2008-01-01

    Full Text Available Esta actividad práctica está pensada para estudiantes angloamericanos de español (nivel C1/C2. Para ellos, hemos diseñado una webquest, es decir, una página de Internet con diferentes apartados cuya finalidad es que el alumno recopile información a partir de la consulta de diferentes páginas web. En ese sentido, el profesor sugiere a sus alumnos unos enlaces electrónicos o urls que aquellos deben visitar.

  12. ¿Cómo se dice me he muerto en inglés? Memory. Una webquest sobre el humor en la clase de ELE

    Directory of Open Access Journals (Sweden)

    Adrián Cabedo Nebot

    2012-08-01

    Full Text Available Esta actividad práctica está pensada para estudiantes angloamericanos de español (nivel C1/C2. Para ellos, hemos diseñado una webquest, es decir, una página de Internet con diferentes apartados cuya finalidad es que el alumno recopile información a partir de la consulta de diferentes páginas web. En ese sentido, el profesor sugiere a sus alumnos unos enlaces electrónicos o urls que aquellos deben visitar.

  13. A novel nuclear pyrometry for the characterization of high-energy bremsstrahlung and electrons produced in relativistic laser-plasma interactions

    International Nuclear Information System (INIS)

    Guenther, M. M.; Sonnabend, K.; Harres, K.; Roth, M.; Brambrink, E.; Vogt, K.; Bagnoud, V.

    2011-01-01

    We present a novel nuclear activation-based method for the investigation of high-energy bremsstrahlung produced by electrons above 7 MeV generated by a high-power laser. The main component is a novel high-density activation target that is a pseudo alloy of several selected isotopes with different photo-disintegration reaction thresholds. The gamma spectrum emitted by the activated targets is used for the reconstruction of the bremsstrahlung spectrum using an analysis method based on Penfold and Leiss. This nuclear activation-based technique allows for the determination of the number of bremsstrahlung photons per energy bin in a wide range energy without any anticipated fit procedures. Furthermore, the analysis method also allows for the determination of the absolute yield, the energy distribution, and the temperature of high-energy electrons at the relativistic laser-plasma interaction region. The pyrometry is sensitive to energies above 7 MeV only, i.e., this diagnostic is insensitive to any low-energy processes.

  14. A novel nuclear pyrometry for the characterization of high-energy bremsstrahlung and electrons produced in relativistic laser-plasma interactions

    Energy Technology Data Exchange (ETDEWEB)

    Guenther, M. M.; Sonnabend, K.; Harres, K.; Roth, M. [Institut fuer Kernphysik, Schlossgartenstr. 9, Technische Universitaet Darmstadt, D-64289 Darmstadt (Germany); Brambrink, E. [Laboratoire pour l' Utilisation des Lasers Intenses, UMR 7605 CNRS-CEA-Ecole Polytechnique-Universite Paris VI, F-91128 Palaiseau (France); Vogt, K.; Bagnoud, V. [GSI - Helmholtzzentrum fuer Schwerionenforschung GmbH, Planckstr. 1, D-64291 Darmstadt (Germany)

    2011-08-15

    We present a novel nuclear activation-based method for the investigation of high-energy bremsstrahlung produced by electrons above 7 MeV generated by a high-power laser. The main component is a novel high-density activation target that is a pseudo alloy of several selected isotopes with different photo-disintegration reaction thresholds. The gamma spectrum emitted by the activated targets is used for the reconstruction of the bremsstrahlung spectrum using an analysis method based on Penfold and Leiss. This nuclear activation-based technique allows for the determination of the number of bremsstrahlung photons per energy bin in a wide range energy without any anticipated fit procedures. Furthermore, the analysis method also allows for the determination of the absolute yield, the energy distribution, and the temperature of high-energy electrons at the relativistic laser-plasma interaction region. The pyrometry is sensitive to energies above 7 MeV only, i.e., this diagnostic is insensitive to any low-energy processes.

  15. The religion under the rule of aesthetics

    Directory of Open Access Journals (Sweden)

    Alberto da Silva Moreira

    2015-04-01

    Full Text Available This article examines the modern process of aestheticization of culture and religion as consequent unfolding of the expansion of market rationality to the subjective life and the libidinal sphere of subjects. Its main objective is to inquire about the future of religion under the impact of sensation seeking culture and the inflation of aesthetics. Firstly, with the help of Türcke, Welsch, Foucault and Schultze´s investigate the aestheticization process of of social life, its causes and characteristics; Secondly, following Dufour, Türcke Leiss, Kline, Jhally e Welsch, it asks how the dynamics of aesthetical impacts the daily life and the bio-psychic economy of people; thirdly, it applies the results obtained to the analysis of what is happening with religion under the regime of aesthetics and sensational culture. Finally, it asks about the possible emancipatory potential of aestheticized own religious experience and tries to draw some further consequences for religion in the aesthetic field.

  16. Avaliação de desempenho e motivação: o impacto das recompensas na motivação e obtenção de melhorias de desempenho, o caso da TranscorSV, S. A

    OpenAIRE

    Reis, Sandra Helena Graça

    2009-01-01

    A realização do presente estudo tem por intuito analisar o impacto das recompensas na motivação e obtenção de melhorias de desempenho. O estudo de caso inerente a este documento recaiu sobre uma empresa de transportes rodoviários de passageiros de Cabo Verde, a “Transcor SV, S.A.”. Os resultados acusaram que neste contexto organizacional, os trabalhadores do escritório e os controladores atribuem um impacto positivo às recompensas na motivação e obtenção de melhorias de desempenho, tendo s...

  17. Proinflammatory Effect of High Glucose Concentrations on HMrSV5 Cells via the Autocrine Effect of HMGB1

    Directory of Open Access Journals (Sweden)

    Yuening Chu

    2017-09-01

    Full Text Available Background: Peritoneal fibrosis, in which inflammation and apoptosis play crucial pathogenic roles, is a severe complication associated with the treatment of kidney failure with peritoneal dialysis (PD using a glucose-based dialysate. Mesothelial cells (MCs take part in the inflammatory processes by producing various cytokines and chemokines, such as monocyte chemoattractant protein 1 (MCP-1 and interleukin 8 (IL-8. The apoptosis of MCs induced by high glucose levels also contributes to complications of PD. High mobility group protein B1 (HMGB1 is an inflammatory factor that has repeatedly been proven to be related to the occurrence of peritoneal dysfunction.Aim: In this study, we aimed to explore the effect and underlying mechanism of endogenous HMGB1 in high-glucose-induced MC injury.Methods: The human peritoneal MC line, HMrSV5 was cultured in high-glucose medium and incubated with recombinant HMGB1. Cellular expression of HMGB1 was blocked using HMGB1 small interfering RNA (siRNA. Apoptosis and production of inflammatory factors as well as the potential intermediary signaling pathways were examined.Results: The major findings of these analyses were: (1 MCs secreted HMGB1 from the nucleus during exposure to high glucose levels; HMGB1 acted in an autocrine fashion on the MCs to promote the production of MCP-1 and IL-8; (2 HMGB1 had little effect on high-glucose-induced apoptosis of the MCs; and (3 HMGB1-mediated MCP-1 and IL-8 production depended on the activation of MAPK signaling pathways. In conclusion, endogenous HMGB1 plays an important role in the inflammatory reaction induced by high glucose on MCs via mitogen-activated protein kinase (MAPK signaling pathways, but it seems to have little effect on high-glucose-induced apoptosis.

  18. Diseño e implementación de un sitio web seguro de consultas para usuaríos de un proveedor de servicios de telecomunicaciones

    OpenAIRE

    Sojos Briones, Ericka Paola; Lopez Montesdeoca, Edwin Daniel; Zambrano Lopez, Ignacio Abel; Espinal Santana, Albert

    2009-01-01

    Los Servidores Web suministran páginas Web a los navegadores (como por ejemplo, Netscape Navigator, Internet Explorer de Microsoft) que lo solicitan. En términos más técnicos, los servidores Web soportan el Protocolo de Transferencia de Hypertexto conocido como HTTP (HyperText Transfer Protocol), el estándar de Internet para comunicaciones Web. Usando HTTP, un servidor Web envía páginas Web en HTML y CGI, así como otros tipos de scripts a los navegadores o browsers cuando éstos lo requieren. ...

  19. Análisis de estructura de sitios web: el caso de las bibliotecas universitarias andaluzas

    OpenAIRE

    Arellano Pardo, María del Carmen; Rodríguez Mateos, David; Nogales Flores, Tomás; Hernández Pérez, Antonio

    1999-01-01

    Se presenta un análisis de la estructura de los sitios web de las bibliotecas universitarias de las ocho universidades andaluzas. Se definen una serie de variables e indicadores necesarios para la medición de diversos aspectos relacionados con estos sitios web. Entre ellos los índices de hipertextualidad, de interconexión y apertura, que relación número y tipo de enlaces con número de páginas. Para la exploración de los diversos servidores web en los que radican las páginas de estas bibliotec...

  20. Diseño e implementación de una web de compra-venta de artículos falleros

    OpenAIRE

    YARZA FERNÁNDEZ, DIEGO

    2015-01-01

    [ES] El mundo fallero es muy importante para la mayoría de los valencianos, tanto es así que se crean incluso páginas de facebook para la compra-venta de artículos falleros. Siguiendo esta idea surgió la de una página web abierta a todos en la que poder vender todo lo que ya no necesites dentro del sector de las fallas y que otros posibles compradores pudieran contactar con ellos y cerrar la venta por su cuenta, sin intermediarios. Una vez determinadas las necesidades de los us...

  1. Uncovering obfuscated web tracking

    OpenAIRE

    Espuña Buxó, Álvaro

    2016-01-01

    En este proyecto creamos una plataforma para detectar automáticamente y de forma dinámica si en una cierta página web se esta usando "canvas fingerprinting" y si el uso de éste está siendo ofuscado. Además analizamos las páginas más visitadas según Alexa y exponemos los resultado obtenidos. In this project we develop a framework that tries to detect automatically and dynamically if a website is using canvas fingerprinting and if its usage is being obfuscated. We also analyze the top ranked...

  2. Contributos para uma crítica da aceleração do tempo

    OpenAIRE

    Duque, Eduardo

    2012-01-01

    Gilles Deleuze (1992: 178) escreveu que nenhum pintor “(...) pinta numa tela virgem, nem o escritor escreve numa página branca, mas a página ou a tela estão desde logo de tal modo cobertas por “clichés” preexistentes, preestabelecidos, que é necessário antes de mais apagar, limpar, laminar, ou até rasgar para fazer passar uma corrente de ar vinda do caos, que nos traz a visão”. Razão pela qual apresentamos, inicialmente, um pequeno poema de Miguel Torga para, de seguida, emp...

  3. Boletín bibliográfico

    OpenAIRE

    Revista, Facultad de Medicina

    2011-01-01

    Parasitosis intestinales, por el doctor Fidel Fernández. Un volumen en cuarto menor de 134 páginas. Salvat Editores, S. A. Barcelona- Buenos Aires. 1940. Doctor Alvarez Herrera. - Tanatología forense. - "Manuales de Medicina Práctica" Salvat Editores, S. A. 1940. Dr. F. Gallart Monés: Ocho lesiones clínicas.- Quinta serie. Un tomo de 212 páginas y 110 fgs. Editorial Salvat. 1939. Enfermedades piogenas y parasitarias de la piel. Por el doctor José Gay Prieto catedrático numerario de Dermatolog...

  4. Differential protein expression, DNA binding and interaction with SV40 large tumour antigen implicate the p63-family of proteins in replicative senescence.

    Science.gov (United States)

    Djelloul, Siham; Tarunina, Marina; Barnouin, Karin; Mackay, Alan; Jat, Parmjit S

    2002-02-07

    P53 activity plays a key role in mammalian cells when they undergo replicative senescence at their Hayflick limit. To determine whether p63 proteins, members of the family of p53-related genes, are also involved in this process, we examined their expression in serially passaged rat embryo fibroblasts. Upon senescence, two truncated DeltaNp63 proteins decreased in abundance whereas two TAp63 isoforms accumulated. 2-D gel analysis showed that the DeltaNp63 proteins underwent post-translational modifications in both proliferating and senescent cells. Direct binding of DeltaNp63 proteins to a p53 consensus motif was greater in proliferating cells than senescent cells. In contrast p63alpha isoforms bound to DNA in a p53 dependent manner and this was higher in senescent cells than proliferating cells. An interaction of p63alpha proteins with SV40 large tumour antigen was also detected and ectopic expression of DeltaNp63alpha can extend the lifespan of rat embryo fibroblasts. Taken together the results indicate that p63 proteins may play a role in replicative senescence either by competition for p53 DNA binding sites or by direct interaction with p53 protein bound to DNA.

  5. El mundo del libro: Julio de 1958

    Directory of Open Access Journals (Sweden)

    Agustín Rodríguez Garavito

    1958-07-01

    Full Text Available Se presentan las reseñas de las siguientes obras y autores: Italia, Guía para vagabundos -- Por Arciniegas, Germán. -- Editorial Suramericana -- 233 páginas. Por los valores del espíritu -- Por Sánchez, Álvaro -- Ediciones del Instituto de Cultura Hispánica. Editorial A B C -- 303 páginas. Colombia Literaria -- Dos Volúmenes -- Litografía Villegas -- División de Extensión Cultural del Ministerio de Educación -- Compilación y trabajo de J.M. Álvarez D'Orsonville.  Temas de castellano. Por Flórez, Luis -- Publicaciones del Instituto Caro y Cuervo. Palabras a la juventud -- Por el doctor Agustín Nieto Caballero --Editorial A B C -- 229 páginas. Peligro en occidente. Por Gonzalo Restrepo Jaramillo. Panamericanismo Democrático. Por Diego Uribe Vargas -- Ediciones Nuevo Signo. Instituciones de Derecho Tributario -- Por A.D. Gianinni -- Editorial de Derecho Financiero de Madrid. Oro y Tormenta -- Por Juana de Ibarborou -- Editorial Zig Zag -- Santiago de Chile. Setenta sonetos del atardecer.

  6. Activation of histamine H4 receptor inhibits TNFα/IMD-0354-induced apoptosis in human salivary NS-SV-AC cells.

    Science.gov (United States)

    Stegajev, Vasili; Kouri, Vesa-Petteri; Salem, Abdelhakim; Rozov, Stanislav; Stark, Holger; Nordström, Dan C E; Konttinen, Yrjö T

    2014-12-01

    Apoptosis is involved in the pathogenesis of Sjögren's syndrome (SS), an autoimmune disease affecting exocrine glands. Our recent studies revealed diminished histamine H4 receptor (H₄R) expression and impaired histamine transport in the salivary gland epithelial cells in SS. The aim was now to test if nanomolar histamine and high-affinity H₄R signaling affect apoptosis of human salivary gland epithelial cell. Simian virus 40-immortalized acinar NS-SV-AC cells were cultured in serum-free keratinocyte medium ± histamine H₄R agonist HST-10. Expression and internalization of H₄R were studied by immunofluorescence staining ± clathrin inhibitor methyl-β-cyclodextrin (MβCD). Apoptosis induced using tumor necrosis factor-α with nuclear factor-κB inhibitor IMD-0354 was studied using phase contrast microscopy, Western blot, flow cytometry and polymerase chain reaction (qRT-PCR). HST-10-stimulated H₄R internalization was inhibited by MβCD. Western blotting revealed diminished phosphorylated c-Jun N-terminal kinase JNK, but unchanged levels of phosphorylated extracellular signal regulated kinase pERK1/2 in H₄R-stimulated samples compared to controls. qRT-PCR showed up-regulated expression of anti-apoptotic B cell lymphoma-extra large/Bcl-xL mRNAs and proteins, whereas pro-apoptotic Bcl-2-associated X protein/BAX remained unchanged in H4R-stimulated samples. H₄R stimulation diminished cleavage of PARP and flow cytometry showed significant dose-dependent inhibitory effect of H₄R stimulation on apoptosis. As far as we know this is the first study showing inhibitory effect of H₄R activation on apoptosis of human salivary gland cells. Diminished H₄R-mediated activation may contribute to loss of immune tolerance in autoimmune diseases and in SS in particular.

  7. A construção discursiva de Dilma Rousseff: análise da primeira página de três jornais mineiros à luz dos estudos sistêmico-funcionais The discursive construction of Dilma Rousseff: a systemic functional analysis of three newspapers?

    Directory of Open Access Journals (Sweden)

    Maria Carmen Aires Gomes

    2012-01-01

    Full Text Available Esta pesquisa analisou a representação da presidente Dilma Rousseff, após o resultado das eleições de 2010 na capa de três jornais mineiros - Estado de Minas, Hoje em dia e Super Notícia -, de acordo com a abordagem Linguística Sistêmico-Funcional apresentada em Halliday (1994 e Halliday e Matthiessen (2004. Objetivo deste artigo é analisar a representação da presidente Dilma Rousseff após o resultado das eleições de 2010, na primeira página de três jornais mineiros, Estado de Minas, Hoje em Dia e Super Notícia, objetivando compreender quais as operações de seleções e escolhas lexicogramaticais foram suscitadas pelas mídias. Cada jornal se revelou ideológico pois deixou transparecer sua linha editorial por meio das escolhas.This research analyzes the representation of Dilma Rousseff, in the front page of three Minas Newspapers, after the results of the 2010 elections - Estado de Minas, Hoje em Dia and Super Notícia -, based on Systemic-Functional Linguistics (Halliday (1994 and Halliday e Matthiessen (2004. Our objective is to understand the ideology underlying the picture construed of the social actor candidate for the presidency. The picture of Dilma Rousseff painted revealed the ideology of the owner - editor - of each newspaper revealed representing the social actor Dilma Rousseff president on your way.

  8. Mejorando la visibilidad de sitios web usando tecnología semántica

    OpenAIRE

    Montenegro, Lenin; Ochoa, Verónica; Espinoza Mejía, Jorge Mauricio; Universidad de Cuenca; Dirección de Investigación de la Universidad de Cuenca; DIUC

    2014-01-01

    La visibilidad de una página Web involucra el proceso de mejora de la posición del sitio en los resultados devueltos por motores de búsqueda como Google. Hay muchas empresas que compiten agresivamente para conseguir la primera posición en los motores de búsqueda más populares. Como regla general, los sitios que aparecen más arriba en los resultados suelen obtener más tráfico a sus páginas, y de esta forma, potencialmente más negocios. En este artículo se describe los principales modelos para ...

  9. Web Munitec, portal de servicios al mutualista

    OpenAIRE

    Cerdán Viu, Julián

    2013-01-01

    El objetivo de la nueva página web es aumentar la usabilidad y accesibilidad de la web, teniendo en cuenta que la mayoría de los usuarios de esta página tienen edades superiores a los 40 años y no tienen gran experiencia a la hora de navegar por internet. Aparte de dar una nueva apariencia a la web, hubo que hacer una reestructuración de los contenidos y menús, aparte de incorporar nuevas secciones. La antigua web, en lo que respecta a servicios a sus mutualistas, únicamente posee una zona ...

  10. Sincronización de estadísticas entre servidores y proxies

    OpenAIRE

    Catullo, María José

    1999-01-01

    Un caching proxy server colecciona todas las páginas que pasan a través de él, así cuando un usuario pregunta por una página, el proxy server, si la tiene, la retoma de su cache, sin necesidad de acceder al servidor real. De ésta manera se ahorra ancho de banda y se agilizan las respuestas. Cada requerimiento que arriba a un proxy ó a un servidor, se almacena en archivos de logs. Existen varios tipos de archivos de logs, que se utilizan para auditoría del rendimiento, monitoreo, control de...

  11. General Office and Information Systems (OGIS)

    OpenAIRE

    Instituto Nacional de Salud

    2009-01-01

    En la figura 1, se observa una disminución de las visitas realizadas en el mes de febrero a las páginas principales del portal virtual del INS con respecto al mes de enero de 2006, esto se explica al rediseño del portal Web y a las nuevas ubicaciones de los accesos rápidos de algunas páginas principales por lo que los usuarios tienen que habituarse al nuevo diseño; La figura 2, muestra una estabilidad parcial de visitas a determinadas secciones de la Revista Peruana de Medicina Experimenta...

  12. Control y privacidad en el ciberespacio. Uso de las cookies por parte de los principales medios digitales españoles.

    OpenAIRE

    García Ull, Francisco José

    2015-01-01

    En marzo de 2012 la legislación española incorporó la directiva comunitaria sobre privacidad electrónica. Desde ese momento, todas las páginas web españolas que recopilan datos a través de cookies y tecnologías similares (siempre que estos archivos no sean estrictamente necesarios para el funcionamiento de la página), tienen la obligación de informar claramente sobre el tipo y la finalidad de los datos recolectados, así como de obtener el consentimiento de los usuarios a destinar sus datos a ...

  13. Análisis cualitativo de la visibilidad de la investigación de las universidades españolas a través de sus páginas web

    Directory of Open Access Journals (Sweden)

    Pinto Molina, María

    2004-09-01

    Full Text Available Analysis and evaluation of the dissemination of the research of Spanish universities throughout the World Wide Web, with the aim of offering a state of their visibility and, derived from it, a set of proposals to improve the quality of such a diffusion. The part of the project related to the use of qualitative research methods is presented. The object of study is conformed by a selected sample of 19 Spanish universities, representing all the Spanish Autonomous Regions but with a consideration to the administrative and scientific weight of some of them. The procedure of definition of the qualitative indicators, the method of data collection and the method of analysis are presented. The results offer a detailed landscape of the visibility of the information on research in the World Wide Web of the universities selected in relation with each of the proposed indicators. Those results have allowed, as well, to define a set of proposals of improvement which could contribute to the excellence of Spanish universities’ web-based information.

    Análisis y evaluación de la difusión de la investigación de las universidades españolas a través de la World Wide Web, con la finalidad de obtener un estado de la visibilidad de la información sobre investigación de las mismas y proponer medidas para mejorar la calidad de su difusión. Se presenta la parte de la investigación relacionada con el uso de métodos de investigación cualitativa. El objeto de estudio está conformado por una muestra seleccionada de 19 universidades españolas, elegidas en función de la representatividad por Comunidad Autónoma y su peso administrativo y científico. Se explica el procedimiento de definición de los indicadores cualitativos, el método de recogida de datos y el procedimiento de análisis. Los resultados ofrecen un panorama detallado del estado de la cuestión de la visibilidad de la información sobre investigación en las páginas web de las

  14. Characterization of flood and precipitation events in Southwestern Germany and stochastic simulation of extreme precipitation (Project FLORIS-SV)

    Science.gov (United States)

    Florian, Ehmele; Michael, Kunz

    2016-04-01

    Several major flood events occurred in Germany in the past 15-20 years especially in the eastern parts along the rivers Elbe and Danube. Examples include the major floods of 2002 and 2013 with an estimated loss of about 2 billion Euros each. The last major flood events in the State of Baden-Württemberg in southwest Germany occurred in the years 1978 and 1993/1994 along the rivers Rhine and Neckar with an estimated total loss of about 150 million Euros (converted) each. Flood hazard originates from a combination of different meteorological, hydrological and hydraulic processes. Currently there is no defined methodology available for evaluating and quantifying the flood hazard and related risk for larger areas or whole river catchments instead of single gauges. In order to estimate the probable maximum loss for higher return periods (e.g. 200 years, PML200), a stochastic model approach is designed since observational data are limited in time and space. In our approach, precipitation is linearly composed of three elements: background precipitation, orographically-induces precipitation, and a convectively-driven part. We use linear theory of orographic precipitation formation for the stochastic precipitation model (SPM), which is based on fundamental statistics of relevant atmospheric variables. For an adequate number of historic flood events, the corresponding atmospheric conditions and parameters are determined in order to calculate a probability density function (pdf) for each variable. This method involves all theoretically possible scenarios which may not have happened, yet. This work is part of the FLORIS-SV (FLOod RISk Sparkassen Versicherung) project and establishes the first step of a complete modelling chain of the flood risk. On the basis of the generated stochastic precipitation event set, hydrological and hydraulic simulations will be performed to estimate discharge and water level. The resulting stochastic flood event set will be used to quantify the

  15. Does prostate brachytherapy treat the seminal vesicles? A dose-volume histogram analysis of seminal vesicles in patients undergoing combined PD-103 prostate implantation and external beam irradiation

    International Nuclear Information System (INIS)

    Stock, Richard G.; Lo, Yeh-Chi; Gaildon, Mohamoud; Stone, Nelson N.

    1999-01-01

    Purpose: Combined brachytherapy of the prostate and external beam irradiation (EBRT) of the prostate and seminal vesicles (SV) is becoming a popular treatment for high-risk prostate cancer. Dose-volume histogram (DVH) analysis of the SV in patients undergoing this treatment was performed to determine the dose distribution to the SV and the adequacy of this treatment in patients with potential SV involvement. Methods and Materials: Twenty-five consecutive patients were treated with a Pd-103 implant of the prostate alone and 45 Gy of EBRT to the prostate and SV. Attempts were not made to implant the SV but seeds were routinely placed at the junction of the prostate and SV. All patients underwent CT-based post implant dosimetric analysis 1 month after implantation. As part of this analysis, DVH were generated for the prostate and total SV volume (SVT). In addition, the SV was divided into 6-mm-thick volumes identified as SV1, SV2, SV3, SV4, and SV5 starting from the junction of the prostate and SV and extending distally. DVH were also generated for these structures. Delivered dose was defined as the D90 (dose delivered to 90% of the organ on DVH). Results: The median volumes in cc of the prostate, SVT, SV1, SV2, SV3, SV4, and SV5 were 34.33, 9.75, 2.7, 3.48, 2.92, 3.18, and 1.96 respectively. The SVT contained from 0-9 seeds (median 2). There was little dose delivered to the SVT and SV volumes from the implanted prostate. The median D90 values for the prostate, SVT, SV1, SV2, SV3, SV4, and SV5 were 8615 cGy, 675 cGy, 3100 cGy, 1329 cGy, 553 cGy, 246 cGy, and 67 cGy, respectively. The dose delivered to the prostate covered small percentages of SV. The percents of SV volumes covered by the prostate D90 were 11, 35, 3.3, 0, 0, and 0 for SVT, SV1, SV2, SV3, SV4, and SV5, respectively. Conclusions: DVH analysis of the SV reveals that dose generated from an implanted prostate contributes little to the SV. Those patients at high risk for SV involvement may be under treated

  16. Cultura indígina e identidade nacional

    Directory of Open Access Journals (Sweden)

    Maria Vitória Silva Paiva

    2016-12-01

    Full Text Available A ideia de uma identidade nacional foi sendo construída; não havia, inicialmente, um Brasil como hoje conhecemos. Essa noção começa a se solidificar entre os anos de 1940 a 1950. As pessoas se identificavam pelo local onde nasciam, onde sua família estava enraizada, não pela sua nacionalidade. A história do país foi e é construída por um processo contínuo de inclusão-exclusão, o que faz com que diferentes grupos sociais e identitários continuem num processo de busca pela sua afirmação, em especial os indígenas e a população afro-brasileira. A metodologia utilizada na construção deste artigo foi a pesquisa bibliográfica e a pesquisa de campo, tanto atuando como psicóloga no grupo de saúde mental que existe dentro da aldeia Jaraguá, em São Paulo, como nas visitas às aldeias Guarani de Paraty, Rio de Janeiro, e entrevistas com três indígenas, das etnias Guarani, Pitaguary e Tibiriça.

  17. Direct advertising to the consumer of web pages in Spanish that offer cannabinoids for medicinal uses

    Directory of Open Access Journals (Sweden)

    Julio Cjuno

    2018-02-01

    Full Text Available Señor Editor: Los canabinoides son sustancias derivadas a partir de las plantas del cannabis (Whiting et al., 2015, las cuales han sido aprobadas por la Food & Drug Admnistration [FDA] para el manejo de diversos síntomas como pérdida de apetito en pacientes con VIH/SIDA, y las náuseas y vómitos asociados a la quimioterapia (OMS, 2015. Sin embargo, un reciente metaanálisis encontró que existe escasa evidencia sobre el uso de canabinoides para diversas condiciones (Whiting et al., 2015. Asimismo, se han reportado eventos adversos tales como mareos, sequedad de boca, náuseas, fatiga, somnolencia, vómitos, desorientación, confusión y alucinaciones (Whiting et al., 2015. Debido a esto, resulta importante que la publicidad dirigida al consumidor [PDAC] realizada por quienes ofertan productos derivados de la marihuana para usos medicinales contenga una adecuada recomendación sobre sus usos y posibles eventos adversos (Gellad and Lyles 2007. Lo cual no ha sido explorado previamente. El objetivo del presente estudio fue evaluar la PDAC de las páginas web que ofrecen derivados de la marihuana para usos medicinales, en países de habla hispana. Para ello, durante marzo del 2017 se realizaron búsquedas en Google.com utilizando los siguientes términos de búsqueda en español: [Marihuana medicinal], [Cannabis medicinal], [Aceite de marihuana], y [Aceite de cannabis], elegidos por ser los términos con más búsquedas sobre el tema en los últimos cinco años según estadísticas de Google Trends (2017, combinadas con nombres de los siguientes países: México, Colombia, Argentina, Chile, Bolivia, Paraguay, Uruguay, Venezuela, Ecuador, Perú y España. El historial y las cookies fueron previamente eliminados para no obtener resultados personalizados. Se revisaron los 50 primeros resultados de cada búsqueda, y se seleccionaron las páginas web que ofrecían derivados de la marihuana con fines medicinales, cuyas características fueron digitadas

  18. La intimidad en el mundo y la gran malla

    Directory of Open Access Journals (Sweden)

    Dra. Rocío Jiménez Ramón

    2001-01-01

    Full Text Available Europeos y asiáticos se sienten cómodos con la idea de permitir que los gobiernos regulen su intimidad, pero la idea de unas leyes amplias que protejan la intimidad pone nerviosos a los norteamericanos.El concepto de confidencialidad de la información abarca las múltiples formas de obtención, utilización y distribución de datos acerca de personas particulares en el ciberespacio. En la vida real, un paseo informal por las calles de una ciudad o por las galerías de un supermercado constituye generalmente un asunto económico, a diferencia de lo que ocurre cuando se navega por la World Wide Web. Como reza una verdad de perogrullo en línea: cuando se navega por la red, la red le navega a uno. Con los programas adecuados, desde un servidor de Internet a menudo se puede identificar el nombre, la dirección electrónica y la ubicación geográfica de la mayoría de usuarios que recalan en una página, así como información sobre otras páginas visitadas recientemente o el modelo de ordenador utilizado. Mientras tanto, muchos creadores de sitios de la web utilizan también etiquetas informáticas denominadas cookies (galletas, que permiten la compilación detallada de registros sobre los intereses individuales de cada visitante, basándose en el tipo de páginas que visitan o en la clase de productos que adquieren al recorrer una página comercial.

  19. Innovaciones en la enseñanza de la Antropología Biológica en la Facultad de Humanidades y Ciencias de la Educación, Universidad de la República, Uruguay

    Directory of Open Access Journals (Sweden)

    Sans, Mónica

    2003-01-01

    Full Text Available La enseñanza de la Antropología Biológica en el Uruguay es impartida como parte de la Licenciatura en Ciencias Antropológicas. Se centra en un curso curricular del mismo nombre, así como en otras asignaturas de carácter opcional que se ofrecen ocasionalmente. Su dictado está organizado en módulos teóricos y prácticos, e incluye temáticas variadas. El número de estudiantes que asiste anualmente es de aproximadamente 70, aunque el número de anotados supera los 100. Uno de los mayores problemas es la falta de bibliografía, lo que se une a la dificultad de los estudiantes para concurrir a las clases teóricas. Con base a esto, se implementó una página web como mecanismo de enseñanza y actualización, que pudiera ser consultada por estudiantes, egresados, y otros interesados. Esta página está incorporada al servidor de nuestra Facultad: http://www.fhuce.edu.uy/antrop/cursos/abiol. Incluye los programas de práctico y teórico, las guías para los prácticos, y vínculos a artículos y páginas web de interés. En el primer año de su puesta on line se detectó que 50% de los estudiantes tenía dificultades con la lectura del inglés, por lo cual se tradujeron varios artículos para cada tema. Aproximadamente el 90% de los estudiantes del año 2003 ha consultado la página.

  20. Asthma control in severe asthmatics under treatment with omalizumab: a cross-sectional observational study in Italy.

    Science.gov (United States)

    Novelli, Federica; Latorre, Manuela; Vergura, Letizia; Caiaffa, Maria Filomena; Camiciottoli, Gianna; Guarnieri, Gabriella; Matucci, Andrea; Macchia, Luigi; Vianello, Andrea; Vultaggio, Alessandra; Celi, Alessandro; Cazzola, Mario; Paggiaro, Pierluigi

    2015-04-01

    Few data are available on the proportion of asthmatics achieving a good asthma control (according GINA guidelines) and on the level of airway inflammation during omalizumab treatment. The aim of this cross-sectional national observational study was to assess the level of control (according to GINA guidelines) achieved in a group of asthmatics on omalizumab treatment, and to characterize the factors that influence the lack of control. We studied 306 asthmatics under omalizumab treatment for a median of 32 months (range 4-120). The level of control according to GINA was good in 25.2%, partial in 47.1% and poor in 24.5% of patients (data were missing for the remaining 3.2%). Comparison between poorly controlled and partially or well controlled asthmatics showed a statistically significant higher prevalence of some comorbidities in the first group, namely obesity, gastro-oesophageal reflux disease (GORD), aspirin intolerance and mental disorders (all p omalizumab treatment, a high percentage of asthmatics obtain a good or partial control of asthma. Comorbidities are associated with the lack of asthma control and persistence of exacerbations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Interactividad, información, promoción y valor de portal de las televisiones locales en la Red

    Directory of Open Access Journals (Sweden)

    Lic. Elvira García de Torres

    2000-01-01

    Full Text Available Tomamos como referencia un trabajo preliminar, presentado en el congreso Comunicar en la Era Digital, que examina la interactividad y la información de las páginas web de las televisiones locales valencianas a partir de un programa de codificación de 72 variables, que se aplica a las páginas de nueve televisiones locales valencianas, la semana del 14 al 21 de febrero de 1999: TVV-Canal 9, Televisión de Elche, Tele-Safor, Televisión de Villena, Canal 55 de Benidorm, Canal Castelló, Valencia-TeVe, Televisión de Silla y Ribera Televisión (Alzira.

  2. Sistemas de recuperación e internet: Metadescripción, procesamiento, webcrawling, técnicas de consulta avanzada, hacking documental y posicionamiento web

    OpenAIRE

    Blázquez Ochando, Manuel

    2013-01-01

    La búsqueda y recuperación en Internet consta de métodos y técnicas complementarios a los empleados en el desarrollo de algoritmos en los motores de búsqueda, véase blog de la asignatura Técnicas Avanzadas de Recuperación de Información. En este sentido, se estudiarán con detenimiento todos los metadatos Dublin Core básicos y extendidos, para su aplicación en páginas web en forma de etiquetas embebidas dentro del código fuente de una página web y en formato RDF. Tales sistemas de meta-descrip...

  3. Application of SV40 T-transformed human corneal epithelial cells to evaluate potential irritant chemicals for in vitro alternative eye toxicity.

    Science.gov (United States)

    Kim, Cho-Won; Park, Geon-Tae; Bae, Ok-Nam; Noh, Minsoo; Choi, Kyung-Chul

    2016-01-01

    Assessment of eye irritation potential is important to human safety, and it is necessary for various cosmetics and chemicals that may contact the human eye. Until recently, the Draize test was considered the standard method for estimating eye irritation, despite its disadvantages such as the need to sacrifice many rabbits for subjective scoring. Thus, we investigated the cytotoxicity and inflammatory response to standard eye irritants using SV40 T-transformed human corneal epithelial (SHCE) cells as a step toward development of an animal-free alternative eye irritation test. MTT and NRU assays of cell viability were performed to investigate the optimal experimental conditions for SHCE cell viability when cells were exposed to sodium dodecyl sulfate (SDS) as a standard eye irritant at 6.25×10(-3) to 1×10(-1)%. Additionally, cell viability of SHCE cells was examined in response to six potential eye irritants, benzalkonium chloride, dimethyl sulfoxide, isopropanol, SDS, Triton X-100 and Tween 20 at 5×10(-3) to 1×10(-1)%. Finally, we estimated the secretion level of cytokines in response to stimulation by eye irritants in SHCE cells. SHCE cells showed a good response to potential eye irritants when the cells were exposed to potential irritants for 10min at room temperature (RT), and cytokine production increased in a concentration-dependent manner, indicating that cytotoxicity and cytokine secretion from SHCE cells may be well correlated with the concentrations of irritants. Taken together, these results suggest that SHCE cells could be an excellent alternative in vitro model to replace in vivo animal models for eye irritation tests. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. Die Deutsche Statistische Gesellschaft in der Weimarer Republik und während der Nazidiktatur

    Science.gov (United States)

    Wilke, Jürgen

    Nach anfänglichen Schwierigkeiten durch den 1. Weltkrieg erlangte die Deutsche Statistische Gesellschaft (DStatG) unter dem renommierten Statistiker und Vorsitzenden der DStatG, Friedrich Zahn, durch eine Vielzahl von Aktivitäten hohes Ansehen. Es gab Bestrebungen, Statistiker aus allen Arbeitsfeldern der Statistik in die DStatG zu integrieren, wobei die "Mathematische Statistik" nur zögerlich akzeptiert wurde (Konjunkturforschung, Zeitreihenanalyse). Nach der Machtübernahme 1933 durch Adolf Hitler geriet die DStatG in das Fahrwasser nationalsozialistischer Ideologie und Politik (Führerprinzip, Gleichschaltung des Vereinswesens). Damit war eine personelle Umstrukturierung in der DStatG verbunden. Politisch Missliebige und rassisch Verfolgte mussten die DStatG verlassen (Bernstein, Freudenberg, Gumbel u.a.). Unter den Statistikern gab es alle Abstufungen im Verhalten zum Regime von Ablehnung und zwangsweiser Anpassung über bereitwilliges Mitläufertum bis zu bewusster Täterschaft. Besonders die Bevölkerungsstatistik wurde durch die NS- Rassenpolitik auf lange Sicht diskreditiert. Im Rahmen von Wirtschaftsplanung und Aufrüstung wurden neue zukunftsträchtige statistische Modelle (Grünig, Bramstedt, Leisse) entwickelt.

  5. Experimental investigation of the photoneutron production out of the high-energy photon fields at linear accelerator

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yeon Su; Yoon, In Ha; Bae, Sun Myeong; Kang, Tae Young; Baek, Geum Mun; Kim, Sung Hwan; Nam, Uk Won; Lee, Jae Jin; Park, Yeong Sik [Dept. of Radiation Oncology, ASAN Medical Center, Seoul (Korea, Republic of)

    2014-12-15

    Photoneutron dose in high-energy photon radiotherapy at linear accelerator increase the risk for secondary cancer. The purpose of this investigation is to evaluate the dose variation of photoneutron with different treatment method, flattening filter, dose rate and gantry angle in radiation therapy with high-energy photon beam (E≥8 MeV). TrueBeam STxTM(Ver1.5, Varian, USA) and Korea Tissue Equivalent Proportional Counter (KTEPC) were used to detect the photoneutron dose out of the high-energy photon field. Complex Patient plans using Eclipse planning system (Version 10.0, Varian, USA) was used to experiment with different treatment technique(IMRT, VMAT), condition of flattening filter and three different dose rate. Scattered photoneutron dose was measured at eight different gantry angles with open field (Field size : 5×5cm). The mean values of the detected photoneutron dose from IMRT and VMAT were 449.7 μSv, 2940.7 μSv. The mean values of the detected photoneutron dose with Flattening Filter(FF) and Flattening Filter Free(FFF) were measured as 2940.7 μSv, 232.0 μSv. The mean values of the photoneutron dose for each test plan (case 1, case 2 and case 3) with FFF at the three different dose rate (400, 1200, 2400 MU/min) were 3242.5 μSv, 3189.4 μSv, 3191.2 μSv with case 1, 3493.2 μSv, 3482.6 μSv, 3477.2 μSv with case 2 and 4592.2 μSv, 4580.0 μSv, 4542.3 μSv with case 3, respectively. The mean values of the photoneutron dose at eight different gantry angles ( 0° , 45°, 90°, 135°, 180°, 225°, 270° , 315°) were measured as 3.2 μSv, 4.3 μSv, 5.3 μSv, 11.3 μSv, 14.7 μSv, 11.2 μSv, 3.7 μSv, 3.0 μSv at 10 MV and as 373.7 μSv, 369.6 μSv, 384.4 μSv, 423.6 μSv, 447.1 μSv, 448.0 μSv, 384.5 μSv, 377.3 μSv at 15MV. As a result, it is possible to reduce photoneutron dose using FFF mode and VMAT method with TrueBeam STxTM. The risk for secondary cancer of the patients will be decreased with continuous evaluation of the photoneutron dose.

  6. Spatially variant morphological restoration and skeleton representation.

    Science.gov (United States)

    Bouaynaya, Nidhal; Charif-Chefchaouni, Mohammed; Schonfeld, Dan

    2006-11-01

    The theory of spatially variant (SV) mathematical morphology is used to extend and analyze two important image processing applications: morphological image restoration and skeleton representation of binary images. For morphological image restoration, we propose the SV alternating sequential filters and SV median filters. We establish the relation of SV median filters to the basic SV morphological operators (i.e., SV erosions and SV dilations). For skeleton representation, we present a general framework for the SV morphological skeleton representation of binary images. We study the properties of the SV morphological skeleton representation and derive conditions for its invertibility. We also develop an algorithm for the implementation of the SV morphological skeleton representation of binary images. The latter algorithm is based on the optimal construction of the SV structuring element mapping designed to minimize the cardinality of the SV morphological skeleton representation. Experimental results show the dramatic improvement in the performance of the SV morphological restoration and SV morphological skeleton representation algorithms in comparison to their translation-invariant counterparts.

  7. How parents perceive screen viewing in their 5-6 year old child within the context of their own screen viewing time: a mixed-methods study.

    Science.gov (United States)

    Thompson, Janice L; Sebire, Simon J; Kesten, Joanna M; Zahra, Jesmond; Edwards, Mark; Solomon-Moore, Emma; Jago, Russell

    2017-06-01

    Few studies have examined parental perceptions of their child's screen-viewing (SV) within the context of parental SV time. This study qualitatively examined parents' perceptions of their 5-6-year-old child's SV within the context of their own quantitatively measured SV. A mixed-methods design employed semi-structured telephone interviews, demographic and SV questionnaires, objectively-measured physical activity and sedentary time. Deductive content analysis was used to explore parents' perceptions of, and concerns about, their child's SV, and management of their child's SV. Comparisons were made between parent-child dyads reporting low (parents were interviewed (94.3% mothers), with 52 interviews analysed. Fifteen parent-child dyads (28.8%) exceeded the 2-h SV threshold on both weekdays and weekend days; 5 parent-child dyads (9.6%) did not exceed this threshold. The remaining 32 dyads reported a combination of parent or child exceeding/not exceeding the SV threshold on either weekdays or weekend days. Three main themes distinguished the 15 parent-child dyads exceeding the SV threshold from the 5 dyads that did not: 1) parents' personal SV-related views and behaviours; 2) the family SV environment; and 3) setting SV rules and limits. Parents in the dyads not exceeding the SV threshold prioritized and engaged with their children in non-SV behaviours for relaxation, set limits around their own and their child's SV-related behaviours, and described an environment supportive of physical activity. Parents in the dyads exceeding the SV threshold were more likely to prioritise SV as a shared family activity, and described a less structured SV environment with minimal rule setting, influenced their child's need for relaxation time. The majority of parents in this study who exceeded the SV threshold expressed minimal concern and a relaxed approach to managing SV for themselves and their child(ren), suggesting a need to raise awareness amongst these parents about the time

  8. How parents perceive screen viewing in their 5–6 year old child within the context of their own screen viewing time: a mixed-methods study

    Directory of Open Access Journals (Sweden)

    Janice L. Thompson

    2017-06-01

    Full Text Available Abstract Background Few studies have examined parental perceptions of their child’s screen-viewing (SV within the context of parental SV time. This study qualitatively examined parents’ perceptions of their 5–6-year-old child’s SV within the context of their own quantitatively measured SV. Methods A mixed-methods design employed semi-structured telephone interviews, demographic and SV questionnaires, objectively-measured physical activity and sedentary time. Deductive content analysis was used to explore parents’ perceptions of, and concerns about, their child’s SV, and management of their child’s SV. Comparisons were made between parent-child dyads reporting low (<2-h per day versus high SV time. Results Fifty-three parents were interviewed (94.3% mothers, with 52 interviews analysed. Fifteen parent-child dyads (28.8% exceeded the 2-h SV threshold on both weekdays and weekend days; 5 parent-child dyads (9.6% did not exceed this threshold. The remaining 32 dyads reported a combination of parent or child exceeding/not exceeding the SV threshold on either weekdays or weekend days. Three main themes distinguished the 15 parent-child dyads exceeding the SV threshold from the 5 dyads that did not: 1 parents’ personal SV-related views and behaviours; 2 the family SV environment; and 3 setting SV rules and limits. Parents in the dyads not exceeding the SV threshold prioritized and engaged with their children in non-SV behaviours for relaxation, set limits around their own and their child’s SV-related behaviours, and described an environment supportive of physical activity. Parents in the dyads exceeding the SV threshold were more likely to prioritise SV as a shared family activity, and described a less structured SV environment with minimal rule setting, influenced their child’s need for relaxation time. Conclusions The majority of parents in this study who exceeded the SV threshold expressed minimal concern and a relaxed approach

  9. Guidance on the Use of Hand-Held Survey Meters for radiological Triage: Time-Dependent Detector Count Rates Corresponding to 50, 250, and 500 mSv Effective Dose for Adult Males and Adult Females

    International Nuclear Information System (INIS)

    Bolch, W.E.; Hurtado, J.L.; Lee, C.; Manger, Ryan P.; Hertel, Nolan; Burgett, E.; Dickerson, W.

    2012-01-01

    In June 2006, the Radiation Studies Branch of the Centers for Disease Control and Prevention held a workshop to explore rapid methods of facilitating radiological triage of large numbers of potentially contaminated individuals following detonation of a radiological dispersal device. Two options were discussed. The first was the use of traditional gamma cameras in nuclear medicine departments operated as makeshift wholebody counters. Guidance on this approach is currently available from the CDC. This approach would be feasible if a manageable number of individuals were involved, transportation to the relevant hospitals was quickly provided, and the medical staff at each facility had been previously trained in this non-traditional use of their radiopharmaceutical imaging devices. If, however, substantially larger numbers of individuals (100 s to 1,000 s) needed radiological screening, other options must be given to first responders, first receivers, and health physicists providing medical management. In this study, the second option of the workshop was investigated by the use of commercially available portable survey meters (either NaI or GM based) for assessing potential ranges of effective dose (G50, 50Y250, 250Y500, and 9500 mSv). Two hybrid computational phantoms were used to model an adult male and an adult female subject internally contaminated with 241Am, 60Cs, 137Cs, 131I, or 192Ir following an acute inhalation or ingestion intake. As a function of time following the exposure, the net count rates corresponding to committed effective doses of 50, 250, and 500 mSv were estimated via Monte Carlo radiation transport simulation for each of four different detector types, positions, and screening distances. Measured net count rates can be compared to these values, and an assignment of one of four possible effective dose ranges could be made. The method implicitly assumes that all external contamination has been removed prior to screening and that the measurements be

  10. Información sobre el tratamiento farmacológico de la obesidad en los sitios web: evaluación de la calidad

    Directory of Open Access Journals (Sweden)

    Emilia Vitoria da SILVA

    2010-12-01

    Full Text Available Pese a que la facilidad de acceso a la información relacionada con la salud en los sitios web puede beneficiar a los pacientes, la información de mala calidad puede a su vez perjudicarlos. El propósito del presente estudio fue probar un modelo de evaluación de las páginas web que contienen información sobre tratamientos farmacológicos contra la obesidad. Se desarrollaron, convalidaron y probaron dos instrumentos: uno de mayor alcance, destinado a investigar la presencia de los criterios técnicos de calidad (CTC y otro, más específico, para evaluar la precisión del contenido y el alcance. La frecuencia de CTC fue baja: sólo en el 39% de las páginas web se declara el autor, el 69% informa la institución responsable de la publicación, el 48% indica la fecha de publicación y el 14% menciona la bibliografía de apoyo. Las calificaciones de la presencia de CTC oscilaron de –3 a 22 (un promedio de 9,4. La exactitud y la exhaustividad de la información no eran satisfactorias: de las 38 páginas evaluadas, 23 contenían información incompleta o divergente en cantidad de datos correctos, las puntuaciones de exactitud oscilaron de –4 a 11 (promedio: 2,82. El método empleado en esta investigación se ha considerado adecuado y puede reproducirse en otros temas relacionados con la salud. En Brasil, las páginas web que contienen información sobre tratamiento farmacológico contra la obesidad son de mala calidad y no son adecuadas como fuentes de información para el público lego.

  11. Normas para publicação

    Directory of Open Access Journals (Sweden)

    Editores INTERthesis

    2014-06-01

    • Sociedade e Meio Ambiente Serão aceitas contribuições nas seguintes modalidades: aArtigo: deve ser original, contribuição de caráter acadêmico e/ou técnico-científico destinada a divulgar resultados de pesquisa científica, de natureza empírica, experimental ou conceitual (mínimo de 12 e máximo de 18 páginas, incluindo títulos, resumos, tabelas, figuras, mapas e referências. b Revisão: avaliação crítica sistematizada da literatura sobre determinado assunto; deve-se citar o objetivo da revisão, especificar (em métodos os critérios de busca na literatura e o universo pesquisado, discutir os resultados obtidos e sugerir estudos no sentido de preencher lacunas do conhecimento atual (mínimo de 12 e máximo de 18 páginas, incluindo títulos, resumos, tabelas, figuras, mapas e referências. cEnsaio: reflexão circunstanciada, com redação adequada ao escopo de uma publicação científica, com maior liberdade por parte do autor para defender determinada posição, que vise aprofundar a discussão ou que apresente nova contribuição/abordagem a respeito de tema relevante (mínimo de 12 e máximo de 18 páginas, incluindo títulos, resumos, tabelas, figuras, mapas e referências. d Resenha: análise crítica sobre livro publicado nos últimos dois anos (até 6 páginas, com as mesmas configurações que se solicita ao texto, incluindo referência bibliográfica completa no cabeçalho. Leia mais nas Instruções aos autores: https://periodicos.ufsc.br/index.php/interthesis/article/view/31715/25904

  12. Computer-aided detection (CAD) of solid pulmonary nodules in chest x-ray equivalent ultralow dose chest CT - first in-vivo results at dose levels of 0.13 mSv

    Energy Technology Data Exchange (ETDEWEB)

    Messerli, Michael, E-mail: Michael.Messerli@usz.ch [Division of Radiology and Nuclear Medicine, Cantonal Hospital St. Gallen (Switzerland); Kluckert, Thomas; Knitel, Meinhard [Division of Radiology and Nuclear Medicine, Cantonal Hospital St. Gallen (Switzerland); Rengier, Fabian [Department of Diagnostic and Interventional Radiology, University Hospital Heidelberg (Germany); Warschkow, René [Department of Surgery, Cantonal Hospital St. Gallen (Switzerland); Alkadhi, Hatem [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, University Zurich (Switzerland); Leschka, Sebastian [Division of Radiology and Nuclear Medicine, Cantonal Hospital St. Gallen (Switzerland); Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, University Zurich (Switzerland); Wildermuth, Simon; Bauer, Ralf W. [Division of Radiology and Nuclear Medicine, Cantonal Hospital St. Gallen (Switzerland)

    2016-12-15

    Highlights: • Computer-aided detection (CAD) of solid pulmonary nodules was compared in 202 patients in standard dose and ultralow dose CT. • The per–nodule sensitivity of CAD was 70% in standard dose CT and 68% in ultralow dose CT. • The per–nodule sensitivity of CAD in standard dose CT was similar to ultralow dose CT in all size subgroups (all p > 0.05). • Adding CAD markings in ultralow dose CT significantly improved the sensitivity of two radiologists from 77% to 88% and from 66% to 79%, respectively. • CAD can serve as an excellent second reader for nodule detection in CT even at dose levels similar to chest X-ray. - Abstract: Objectives: To determine the value of computer-aided detection (CAD) for solid pulmonary nodules in ultralow radiation dose single-energy computed tomography (CT) of the chest using third-generation dual-source CT at 100 kV and fixed tube current at 70 mAs with tin filtration. Methods: 202 consecutive patients undergoing clinically indicated standard dose chest CT (1.8 ± 0.7 mSv) were prospectively included and scanned with an additional ultralow dose CT (0.13 ± 0.01 mSv) in the same session. Standard of reference (SOR) was established by consensus reading of standard dose CT by two radiologists. CAD was performed in standard dose and ultralow dose CT with two different reconstruction kernels. CAD detection rate of nodules was evaluated including subgroups of different nodule sizes (<5, 5–7, >7 mm). Sensitivity was further analysed in multivariable mixed effects logistic regression. Results: The SOR included 279 solid nodules (mean diameter 4.3 ± 3.4 mm, range 1–24 mm). There was no significant difference in per–nodule sensitivity of CAD in standard dose with 70% compared to 68% in ultralow dose CT both overall and in different size subgroups (all p > 0.05). CAD led to a significant increase of sensitivity for both radiologists reading the ultralow dose CT scans (all p < 0.001). In multivariable analysis, the use

  13. Computer-aided detection (CAD) of solid pulmonary nodules in chest x-ray equivalent ultralow dose chest CT - first in-vivo results at dose levels of 0.13 mSv

    International Nuclear Information System (INIS)

    Messerli, Michael; Kluckert, Thomas; Knitel, Meinhard; Rengier, Fabian; Warschkow, René; Alkadhi, Hatem; Leschka, Sebastian; Wildermuth, Simon; Bauer, Ralf W.

    2016-01-01

    Highlights: • Computer-aided detection (CAD) of solid pulmonary nodules was compared in 202 patients in standard dose and ultralow dose CT. • The per–nodule sensitivity of CAD was 70% in standard dose CT and 68% in ultralow dose CT. • The per–nodule sensitivity of CAD in standard dose CT was similar to ultralow dose CT in all size subgroups (all p > 0.05). • Adding CAD markings in ultralow dose CT significantly improved the sensitivity of two radiologists from 77% to 88% and from 66% to 79%, respectively. • CAD can serve as an excellent second reader for nodule detection in CT even at dose levels similar to chest X-ray. - Abstract: Objectives: To determine the value of computer-aided detection (CAD) for solid pulmonary nodules in ultralow radiation dose single-energy computed tomography (CT) of the chest using third-generation dual-source CT at 100 kV and fixed tube current at 70 mAs with tin filtration. Methods: 202 consecutive patients undergoing clinically indicated standard dose chest CT (1.8 ± 0.7 mSv) were prospectively included and scanned with an additional ultralow dose CT (0.13 ± 0.01 mSv) in the same session. Standard of reference (SOR) was established by consensus reading of standard dose CT by two radiologists. CAD was performed in standard dose and ultralow dose CT with two different reconstruction kernels. CAD detection rate of nodules was evaluated including subgroups of different nodule sizes (<5, 5–7, >7 mm). Sensitivity was further analysed in multivariable mixed effects logistic regression. Results: The SOR included 279 solid nodules (mean diameter 4.3 ± 3.4 mm, range 1–24 mm). There was no significant difference in per–nodule sensitivity of CAD in standard dose with 70% compared to 68% in ultralow dose CT both overall and in different size subgroups (all p > 0.05). CAD led to a significant increase of sensitivity for both radiologists reading the ultralow dose CT scans (all p < 0.001). In multivariable analysis, the use

  14. First molecular data and the phylogenetic position of the millipede-like centipede Edentistoma octosulcatum Tömösváry, 1882 (Chilopoda: Scolopendromorpha: Scolopendridae.

    Directory of Open Access Journals (Sweden)

    Varpu Vahtera

    Full Text Available Edentistoma octosulcatum Tömösváry, 1882, is a rare, superficially millipede-like centipede known only from Borneo and the Philippines. It is unique within the order Scolopendromorpha for its slow gait, robust tergites, and highly modified gizzard and mandible morphology. Not much is known about the biology of the species but it has been speculated to be arboreal with a possibly vegetarian diet. Until now its phylogenetic position within the subfamily Otostigminae has been based only on morphological characters, being variably ranked as a monotypic tribe (Arrhabdotini or classified with the Southeast Asian genus Sterropristes Attems, 1934. The first molecular data for E. octosulcatum sourced from a newly collected specimen from Sarawak were analysed with and without morphology. Parsimony analysis of 122 morphological characters together with two nuclear and two mitochondrial loci resolves Edentistoma as sister group to three Indo-Australian species of Rhysida, this clade in turn grouping with Ethmostigmus, whereas maximum likelihood and parsimony analyses of the molecular data on their own ally Edentistoma with species of Otostigmus. A position of Edentistoma within Otostigmini (rather than being its sister group as predicted by the Arrhabdotini hypothesis is consistently retrieved under different analytical conditions, but support values within the subfamily remain low for most nodes. The species exhibits strong pushing behaviour, suggestive of burrowing habits. Evidence against a suggested vegetarian diet is provided by observation of E. octosulcatum feeding on millipedes in the genus Trachelomegalus.

  15. Análise de campo do desempenho de acessos Web em IPv6.

    OpenAIRE

    Pirani, Deivis Fernandes

    2014-01-01

    Este trabalho apresenta um estudo de campo, comparativo, sobre a utilização dos protocolos IPv4 e IPv6 no acesso a páginas web. Foi considerada a perspectiva de um usuário real deste tipo de serviço, no atual período de transição do IPv4 para o IPv6. Para que tal estudo pudesse ser realizado, foi desenvolvido um arranjo no qual três configurações de endereçamento do Protocolo de Internet, IPv4, IPv6 e Pilha Dupla (IPv4+IPv6), foram utilizadas nos acessos às 100 páginas web, com suporte ao I v...

  16. Dose assessment of natural radioactivity in fly ash and environmental materials from Morupule a coal-fired power station in Botswana

    International Nuclear Information System (INIS)

    Mudiwa, J

    2015-01-01

    This study has been undertaken to estimate the occupational and public radiation doses due to natural radioactivity at Morupule, a Coal-Fired Power Station and its environs. The radiation doses were reconstructed to include 60 year period from 1985 to 2045. Direct gamma ray spectroscopy was used to determine the natural radionuclides Th-232, U-238, and K-40 both qualitatively and quantitatively for fly ash, coal, soil and water (from the fly ash ponds) samples. The average activity concentrations for Th-232, U-238, and K-40 in fly ash samples were 64.54 Bq/kg, 49.37 Bq/kg and 40.08 Bq/kg respectively. In the case of coal, the corresponding average activity concentrations for Th-232, U-238, and K-40 were 27.43 Bq/kg, 18.10 Bq/kg and 17.38 Bq/kg respectively. For soil samples, the average activity concentrations for Th-232, U-238, and K-40 were 10.11 Bq/kg, 6.76 Bq/kg and 118.03 Bq/kg respectively. In water samples, the average activity concentrations for Th-232, U-238, and K-40 were 0.79 Bq/l, 0.32 Bq/l and 1.01 Bq/l respectively. These average activity concentrations were generally comparable to the average world activity concentrations in the case of coal samples, but were generally lower than the average world activity concentrations in the case of fly ash, soil and water samples. The average annual effective doses for the study area were estimated as 0.320 mSv, 0.126 mSv, 0.069 mSv and 0.003 mSv for fly ash, coal, soil and water samples respectively. Dose reconstruction modelling estimated the average fly ash annual effective doses for the years 1985, 1995, 2005, 2015, 2025, 2035 and 2045 to be 0.182 mSv, 0.459 mSv, 0.756 mSv, 0.320 mSv, 0.183 mSv, 0.137 mSv and 0.124 mSv respectively. The reconstructed average coal annual effective doses for similar years were 0.070 mSv, 0.182 mSv, 0.303 mSv, 0.126 mSv, 0.070 mSv, 0.060 mSv and 0.046 mSv respectively. The dose reconstruction modelling also estimated the average soil annual effective doses for the same years as

  17. Genomic evidence that simian virus 2 and six other simian picornaviruses represent a new genus in Picornaviridae

    International Nuclear Information System (INIS)

    Oberste, M. Steven; Maher, Kaija; Pallansch, Mark A.

    2003-01-01

    Analysis of the VP1 capsid protein coding region of simian virus (SV) 2, SV16, SV18, SV42, SV44, SV45, and SV49 demonstrates that they are clearly distinct from members of the Enterovirus genus and from members of other existing picornavirus genera. To further characterize this group of viruses and to clarify their classification within the Picornaviridae, we have determined the complete genomic sequence of SV2 (8126 nucleotides). The genome was typical of members of Picornaviridae, encoding a single open reading frame. The putative polyprotein contained typical picornavirus protease cleavage sites, yielding mature proteins homologous to each of the known picornavirus proteins. SV2 contained an amino-terminal extension of the reading frame, which was analogous to the leader protein of members of the Aphthovirus, Cardiovirus, Erbovirus, Kobuvirus, and Teschovirus genera, but there was no significant amino acid homology with any of these known leader proteins. The 2A protein also aligned poorly with the 2A proteins of other picornaviruses. The deduced amino acid sequences of the SV2 structural and nonstructural proteins were related to but phylogenetically distinct from those of enteroviruses and human rhinoviruses. The major distinguishing features of SV2 were the presence of a type 2 internal ribosome entry site in the 5'-NTR, a putative leader protein encoded upstream of the structural proteins, and an unusually large 2A protein. On the basis of the molecular analysis, we propose that SV2, SV16, SV18, SV42, SV44, SV45, SV49, and porcine enterovirus 8 be classified as members of a new genus in Picornaviridae and that SV2 (strain 2383) be designated as the type strain

  18. Variations in absorbed doses from 59Fe in different diseases

    International Nuclear Information System (INIS)

    Roth, P.; Werner, E.; Henrichs, K.; Elsasser, U.; Kaul, A.

    1986-01-01

    The biokinetics of radiopharmaceuticals administered in vivo may vary considerably with changes in organ functions. They studied the variations in absorbed doses from 59 Fe in 207 patients with different diseases, in whom ferrokinetic investigations were performed for diagnostic purposes. Radiation doses to the bone marrow were highest in patients with deserythropoietic anemias (mean 38 nSv/Bq, range 19 - 57 nSv/Bq) and in hemolytic anemias (mean 21 nSv/Bq, range 7 - 35 nSv/Bq), whereas lower and rather constant values were found in other diseases (mean values between 9 and 13 nSv/Bq). The highest organ doses, the greatest differences with respect to diagnosis and also the largest variations within each group of patients were found for liver and spleen (e. g. in aplastic anemia; liver: 66 nSv/Bq, range 29 - 104 nSv/Bq; spleen: 57 nSv/Bq, range 34 - 98 nSv/Bq. In iron deficiency; liver: 13 nSv/Bq range 12 - 14 nSv/q; spleen: 19 nSv/Bq, range 18 - 20 nSv/Bq). Lower organ doses and smaller variations within and between the groups of patients were found for the gonads (means 3 - 7 nSv/Bq), the kidneys (means 10 - 13 nSv/Bq), the bone (means 4 - 7 nSv/Bq), the lung (means 8 - 12 nSv/Bq), and the total body (means 6 - 8 nSv/Bq). In patients with chronic bleeding absorbed doses decrease concomitantly to the extent of blood loss. The D/sub E/ is not markedly affected by the variations in organ doses but is fairly constant for different diseases. 16 references, 1 figure, 3 tables

  19. 75 FR 17715 - Notice of Receipt of a Pesticide Petition Filed for Temporary Tolerance Exemption for Residues of...

    Science.gov (United States)

    2010-04-07

    ... Facility telephone number is (703) 305-5805. FOR FURTHER INFORMATION CONTACT: Gina Casciano, Biopesticides... requirements. Dated: March 30, 2010. Keith A. Matthews, Acting Director, Biopesticides and Pollution Prevention...

  20. 76 FR 17644 - Pesticide Product; Registration Application

    Science.gov (United States)

    2011-03-30

    ... telephone number is (703) 305-5805. FOR FURTHER INFORMATION CONTACT: Gina Casciano, Biopesticides and... Environmental protection, Pesticides and pest. Dated: March 18, 2011. Keith A. Matthews, Director, Biopesticides...

  1. 75 FR 53689 - Issuance of an Experimental Use Permit

    Science.gov (United States)

    2010-09-01

    ... INFORMATION CONTACT: Gina Casciano, Biopesticides and Pollution Prevention Division (7511P), Office of..., Experimental use permits. Dated: August 19, 2010. W. Michael McDavit, Acting Director, Biopesticides and...

  2. Quantitative assessment of global and regional air trappings using non-rigid registration and regional specific volume change of inspiratory/expiratory CT scans: Studies on healthy volunteers and asthmatics

    International Nuclear Information System (INIS)

    Lee, Eun Sol; Seo, Joon Beom; Lee, Hyun Joo; Chae, Eun Jin; Lee, Sang Min; Oh, Sang Young; Kim, Nam Kug

    2015-01-01

    The purpose of this study was to compare air trapping in healthy volunteers with asthmatics using pulmonary function test and quantitative data, such as specific volume change from paired inspiratory CT and registered expiratory CT. Sixteen healthy volunteers and 9 asthmatics underwent paired inspiratory/expiratory CT. DeltaSV, which represents the ratio of air fraction released after exhalation, was measured with paired inspiratory and anatomically registered expiratory CT scans. Air trapping indexes, DeltaSV0.4 and DeltaSV0.5, were defined as volume fraction of lung below 0.4 and 0.5 DeltaSV, respectively. To assess the gravity effect of air-trapping, DeltaSV values of anterior and posterior lung at three different levels were measured and DeltaSV ratio of anterior lung to posterior lung was calculated. Color-coded DeltaSV map of the whole lung was generated and visually assessed. Mean DeltaSV, DeltaSV0.4, and DeltaSV0.5 were compared between healthy volunteers and asthmatics. In asthmatics, correlation between air trapping indexes and clinical parameters were assessed. Mean DeltaSV, DeltaSV0.4, and DeltaSV0.5 in asthmatics were significantly higher than those in healthy volunteer group (all p < 0.05). DeltaSV values in posterior lung in asthmatics were significantly higher than those in healthy volunteer group (p = 0.049). In asthmatics, air trapping indexes, such as DeltaSV0.5 and DeltaSV0.4, showed negative strong correlation with FEF25-75, FEV1, and FEV1/FVC. DeltaSV map of asthmatics showed abnormal geographic pattern in 5 patients (55.6%) and disappearance of anterior-posterior gradient in 3 patients (33.3%). Quantitative assessment of DeltaSV (the ratio of air fraction released after exhalation) shows the difference in extent of air trapping between health volunteers and asthmatics.

  3. Quantitative assessment of global and regional air trappings using non-rigid registration and regional specific volume change of inspiratory/expiratory CT scans: Studies on healthy volunteers and asthmatics

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Sol; Seo, Joon Beom; Lee, Hyun Joo; Chae, Eun Jin; Lee, Sang Min; Oh, Sang Young; Kim, Nam Kug [Dept. of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of)

    2015-06-15

    The purpose of this study was to compare air trapping in healthy volunteers with asthmatics using pulmonary function test and quantitative data, such as specific volume change from paired inspiratory CT and registered expiratory CT. Sixteen healthy volunteers and 9 asthmatics underwent paired inspiratory/expiratory CT. DeltaSV, which represents the ratio of air fraction released after exhalation, was measured with paired inspiratory and anatomically registered expiratory CT scans. Air trapping indexes, DeltaSV0.4 and DeltaSV0.5, were defined as volume fraction of lung below 0.4 and 0.5 DeltaSV, respectively. To assess the gravity effect of air-trapping, DeltaSV values of anterior and posterior lung at three different levels were measured and DeltaSV ratio of anterior lung to posterior lung was calculated. Color-coded DeltaSV map of the whole lung was generated and visually assessed. Mean DeltaSV, DeltaSV0.4, and DeltaSV0.5 were compared between healthy volunteers and asthmatics. In asthmatics, correlation between air trapping indexes and clinical parameters were assessed. Mean DeltaSV, DeltaSV0.4, and DeltaSV0.5 in asthmatics were significantly higher than those in healthy volunteer group (all p < 0.05). DeltaSV values in posterior lung in asthmatics were significantly higher than those in healthy volunteer group (p = 0.049). In asthmatics, air trapping indexes, such as DeltaSV0.5 and DeltaSV0.4, showed negative strong correlation with FEF25-75, FEV1, and FEV1/FVC. DeltaSV map of asthmatics showed abnormal geographic pattern in 5 patients (55.6%) and disappearance of anterior-posterior gradient in 3 patients (33.3%). Quantitative assessment of DeltaSV (the ratio of air fraction released after exhalation) shows the difference in extent of air trapping between health volunteers and asthmatics.

  4. 75 FR 33506 - Safety and Security Zones; Tall Ships Challenge 2010, Great Lakes, Cleveland, OH, Bay City, MI...

    Science.gov (United States)

    2010-06-14

    ... ``small entities'' comprises small businesses and not-for-profit organizations that are independently... Entities Under section 213(a) of the Small Business Regulatory Enforcement Fairness Act of 1996 (Pub. L... BALTIMORE II, S/V ROALD AMUNDSEN, S/V RED WITCH, S/V ROTALISTE, S/V ROSEWAY, S/V UNICORN, S/V WELCOME, and S...

  5. Modelling of SH- and P-SV-wave fields and seismic microzonation based on response spectra for Talchir basin, India

    International Nuclear Information System (INIS)

    Mohanty, W.K.; Yanger Walling, M.; Vaccari, F.; Tripathy, T.; Panza, G.F.

    2008-02-01

    The P-SV- and SH-wave field in the Talchir basin is simulated along eight profiles: four profiles strike across the basin and the other four are along the basin. The hybrid method, which combines two computational techniques, modal Summation and finite differences, is used to produce multiphase synthetic seismograms. An M = 6 earthquake is considered, with hypocenter along the North Orissa Boundary Fault (NOBF) at a depth of 5 km and with the focal mechanisms parameters: dip = 90 deg., strike = 160 deg. and rake = 180 deg. The peak acceleration (AMAX) along each profile is determined considering the maximum acceleration obtained at the horizontal components. The response spectra ratio (RSR) as a function of frequency is computed for the eight profiles and the higher amplification is seen to increase in correspondence with the thicker sedimentary cover, especially for the radial component. Higher site amplification for all the profiles is observed in the frequency range from 0.5 to 1.4 Hz. To validate the obtained site-effects, the sources, for the profiles across the basin, are placed near to the southern end of the profile and the site amplifications are recomputed. Even if the spatial distribution of AMAX is mainly controlled by the epicentral distance, i.e. the geometrical spreading prevails on local soil effects, the RSR shows a pattern that can be easily correlated with the local site conditions. The RSR at the intersection of the profiles is dependent not only upon the local lithology and sediment thickness but also upon the epicentral distance. The Talchir basin is classified into three zones based on the RSR values: low RSR zone (1.0 - 1.9), intermediate RSR zone (2.0 - 2.8) and high RSR zone (2.9 - 5.2). The PGA estimated for the bedrock model by Bhatia et al. (1999) for the study region is around 0.05 to 0.10 g while the Indian seismic zonation map estimated it to be in the range from 0.1 to 0.2g. In the present study, that considers the effects of

  6. Epidermal cell-shape regulation and subpopulation kinetics during butyrate-induced terminal maturation of normal and SV40-transformed human keratinocytes: epithelial models of differentiation therapy.

    Science.gov (United States)

    Staiano-Coico, L; Steinberg, M; Higgins, P J

    1990-10-15

    Recent data indicate that malignant human epidermal cells may be appropriate targets for sodium butyrate (NaB)-mediated differentiation therapy. The response of pre- and post-crisis populations of SV40-transformed human keratinocytes (SVKs) to this differentiation-inducing agent was assessed, therefore, within the framework of NaB-directed normal human keratinocyte (NHK) maturation. NaB augmented cornified envelope (CE) production in NHK and pre-crisis SVK cultures; the time-course and efficiency of induced maturation were similar in the 2 cell systems. In NHKs, the percentage of amplifying ("B" substate) cells decreased with time in NaB correlating with increases in both "C" stage keratinocytes and CEs. The latter formed over one or 2 layers of nucleated basal-like cells. Inductions were accompanied by immediate cell cycle blocks (in both the G1 and G2/M phases), reorganization within the actin cytoskeleton, and transient early increases in cellular actin content. Increased NHK and pre-crisis SVK cytoskeletal-associated actin reached a maximum approximately 48 hr after NaB addition and preceded development of CEs. The CE precursors, thus, probably reside in the "B" substate. Post-crisis SVKs, in contrast, were refractive to NaB-induced terminal maturation or cell-cycle perturbation, failed to initiate actin filament rearrangements, and retained a basal cell-like phenotype. Stable transformation of human SVKs in post-crisis phase, therefore, appears to be associated with loss of maturation "competence" within the "B" keratinocyte subpopulation.

  7. Insertion of liver enriched transcription factor hepatocyte nuclear factor-4 (HNF-4) in a vector which contains simian virus (SV40) promoter

    International Nuclear Information System (INIS)

    Al-Nbaheen, M.; Pourzand, C.; Tyrrell, R.M.

    2006-01-01

    One way of targeting gene expression in vivo is to control transcription using a tissue-specific regulatory system. Tissue specific promoters or enhancers are in use in transgenic animals and could be utilized in medical for gene therapy. At present the usual method for selection of a tissue-specific promoter is to identify a gene, which is expressed at unusually high level in the target tissue, and then to use the promoter for this gene to drive expression of another therapeutic gene in the target tissue. This approach is logical but does not always lead to high levels of gene expression. A second approach is to investigate the scope for discovery of synthetic specific promoters using a target tissue. The objective of the work described in this paper was to use both approach to design plasmid DNA expression vectors that would carry liver-specific promoter/enhancer linked to reporter gene (i.e. luciferase). Then transfect these vectors to both liver-derived and non-liver cell lines. This is followed by evaluation of the liver-specificity of each construct by measuring the basal level expression of the reporter gene (i.e. luciferase activity) in both cell lines. Hepatocyte nuclear factor-4 (HNF-4) is liver-enriched transcription factor used to design new synthetic enhancers by inserting a tandem array of 1', 3' or 5' repeats of the HNF-4 binding site upstream of the SV40 promoter linked to the luciferase reporter gene within an Epstein-Barr virus (EBV)-based vector, p 706. The results of transfection revealed that unexpectedly the HNF-4 binding sites in these constructs act as a repressor rather than enhancer of the liver-specific expression of the luciferase gene. (author)

  8. La Ampliación de los Canales de Comunicación entre Las Universidades Públicas Federales y La Sociedad: Los Portales Insitucionales como Mecanismos para Implementar un Nuevo Modelo de Gobernanza.

    Directory of Open Access Journals (Sweden)

    Priscila Valduga Dinarte

    2015-12-01

    las páginas de las universidades federales. Como método de procedimiento fue tomado el monográfico, en vista de la pesquisa en las páginas de esas instituciones. Además, como técnicas de pesquisa fueron utilizadas la observación directa, estructurada y no participante, aún la bibliográfica, pues se vale de autores para esclarecer y embazar la temática. Fue concluso que aún hay un largo camino a ser recorrido para que las universidades federales públicas brasileñas efectivamente adentren en la era del acceso, una vez que la implementación de la LAI en los espacios académicos aún está en inicio.

  9. Sobre el Boletín

    Directory of Open Access Journals (Sweden)

    Andrés Arias

    2007-04-01

    Full Text Available En la página de Internet de la Biblioteca Luis Ángel Arango se indica que una de la funciones principales del Boletín es la de reseñar la novedades bibliográfica que van apareciendo en el país. "Novedades": de lo nuevo, tanto en las publicaciones como en las reseñas. Es decir, si lo que asegura la página de la Biblioteca es cierto, quien se acerca al Boletín debe encontrarse con comentarios recientes sobre libros recientes. Con guía que le ayuden a elegir entre las mil ofertas que hay en el mercado bibliográfico. Pero bien sabemos que este objetivo no se cumple.

  10. Asthma-COPD overlap syndrome-Coexistence of chronic obstructive pulmonary disease and asthma in elderly patients and parameters for their differentiation.

    Science.gov (United States)

    Tochino, Yoshihiro; Asai, Kazuhisa; Shuto, Taichi; Hirata, Kazuto

    2017-03-01

    Japan is an aging society, and the number of elderly patients with asthma and chronic obstructive pulmonary disease (COPD) is consequently increasing, with an estimated incidence of approximately 5 million. In 2014, asthma-COPD overlap syndrome (ACOS) was defined by a joint project of Global Initiative for Asthma (GINA) committee and the Global Initiative for Chronic Obstructive Lung Disease (GOLD) committee. The main aims of this consensus-based document are to assist clinicians, especially those in primary care or nonpulmonary specialties. In this article, we discussed parameters to differentiate asthma and COPD in elderly patients and showed prevalence, clinical features and treatment of ACOS on the basis of the guidelines of GINA and GOLD. Furthermore, we showed also referral for specialized investigations.

  11. gina Web gestió protectora d'animals

    OpenAIRE

    Segura Valls, Marc

    2011-01-01

    Aplicació Web amb ASP visual studio 2010 i Visual C# , Microsoft SQL Server i AJAX. Aplicación Web con ASP visual studio 2010 y Visual C# , Microsoft SQL Server y AJAX. ASP Web Application with Visual Studio 2010 and Visual C #, Microsoft SQL Server and AJAX.

  12. Geophysical fingerprints of shallow cultural structures from microgravity and GPR measurements in the Church of St. George, Svätý Jur, Slovakia

    Science.gov (United States)

    Panisova, Jaroslava; Murín, Igor; Pašteka, Roman; Haličková, Jana; Brunčák, Peter; Pohánka, Vladimír; Papčo, Juraj; Milo, Peter

    2016-04-01

    Recording of the historic edifice using the state-of-the-art geodetic and geophysical techniques brings easier visualisation in form of a three-dimensional (3D) model, thus allowing better understanding of its historical construction by the public and non-experts. We have applied this approach at the Church of St. George, one of the most significant religious buildings in south-western Slovakia, which dominates a silhouette of the town Svätý Jur. The geodetic survey allowed to record the actual state of the church. The church exterior was surveyed using a total station. Due to the intricate shape of the interior components of the church, it was decided to use a terrestrial laser scanner to generate the point cloud data, which were processed into floor plan, elevations, sectional 2D drawings and 3D model. The geophysical survey was carried out in the interior of the church in order to identify potential subsurface anthropogenic structures. Microgravity and ground penetrating radar (GPR) methods were selected as the most effective geophysical tools for such task. In microgravity data processing we focused on the calculation and removal of the gravitational effects of the building masses. The main negative gravity anomalies of interest in the nave, which also have been confirmed by GPR measurements, are interpreted as medieval crypts. Another very important outcome of the geophysical survey is the discovery of the west wall foundations of the oldest Romanesque construction. From each geophysical data acquired we derived 3D polygonal models, which are compared to achieve more realistic picture of the subsurface structures. Verification of these structures by means of archaeological excavation has not been carried out yet.

  13. Defining the diverse spectrum of inversions, complex structural variation, and chromothripsis in the morbid human genome.

    Science.gov (United States)

    Collins, Ryan L; Brand, Harrison; Redin, Claire E; Hanscom, Carrie; Antolik, Caroline; Stone, Matthew R; Glessner, Joseph T; Mason, Tamara; Pregno, Giulia; Dorrani, Naghmeh; Mandrile, Giorgia; Giachino, Daniela; Perrin, Danielle; Walsh, Cole; Cipicchio, Michelle; Costello, Maura; Stortchevoi, Alexei; An, Joon-Yong; Currall, Benjamin B; Seabra, Catarina M; Ragavendran, Ashok; Margolin, Lauren; Martinez-Agosto, Julian A; Lucente, Diane; Levy, Brynn; Sanders, Stephan J; Wapner, Ronald J; Quintero-Rivera, Fabiola; Kloosterman, Wigard; Talkowski, Michael E

    2017-03-06

    Structural variation (SV) influences genome organization and contributes to human disease. However, the complete mutational spectrum of SV has not been routinely captured in disease association studies. We sequenced 689 participants with autism spectrum disorder (ASD) and other developmental abnormalities to construct a genome-wide map of large SV. Using long-insert jumping libraries at 105X mean physical coverage and linked-read whole-genome sequencing from 10X Genomics, we document seven major SV classes at ~5 kb SV resolution. Our results encompass 11,735 distinct large SV sites, 38.1% of which are novel and 16.8% of which are balanced or complex. We characterize 16 recurrent subclasses of complex SV (cxSV), revealing that: (1) cxSV are larger and rarer than canonical SV; (2) each genome harbors 14 large cxSV on average; (3) 84.4% of large cxSVs involve inversion; and (4) most large cxSV (93.8%) have not been delineated in previous studies. Rare SVs are more likely to disrupt coding and regulatory non-coding loci, particularly when truncating constrained and disease-associated genes. We also identify multiple cases of catastrophic chromosomal rearrangements known as chromoanagenesis, including somatic chromoanasynthesis, and extreme balanced germline chromothripsis events involving up to 65 breakpoints and 60.6 Mb across four chromosomes, further defining rare categories of extreme cxSV. These data provide a foundational map of large SV in the morbid human genome and demonstrate a previously underappreciated abundance and diversity of cxSV that should be considered in genomic studies of human disease.

  14. Personal dose assessment using region of interest analysis compared with harshaw TLD WinREMS software evaluation

    International Nuclear Information System (INIS)

    Adjei, D.

    2010-06-01

    Personal dose equivalents, Hp(10), have been evaluated manually using Region of Interest (ROI) analysis and compared with the automated computerized WinREMS software for the occupationally exposed in medical, industrial and research/teaching applications for 2008 and 2009. The mean annual effective dose estimated by the WinREMS software for medical, industrial and research/teaching applications for the study period are 0.459 mSv, 0.549mSv and 0.447 mSv, respectively compared with ROI analysis are 0.424 mSv, 0.520 mSv and 0.407 mSv respectively. The mean annual collective doses evaluated by the WinREMS software for medical, industrial and research/teaching applications for the two-year study period are 0.258 man-Sv, 0.084 man-Sv and 0.032 man-Sv respectively, compared with the ROI analysis with values: 0.238 man-Sv, 0.080 man-Sv and 0.029 man-Sv respectively. The individual doses for the occupationally exposed in Ghana fall within the typical range of individual doses in the UNSCEAR 2008 report. In calibration mode, the WinREMS method overestimated the personal dose equivalent by 51.3% for doses below 1 mSv and 12.0% above 1 mSv. The corresponding values for the Region of Interest analysis method are 13.2% and 6.5%. The results from the study indicate that the ROI analysis provides a better alternative to estimating the personal doses (au).

  15. Absorbed and effective dose from periapical radiography by portable intraoral x-ray machine

    International Nuclear Information System (INIS)

    Cho, Jeong Yeon; Han, Won Jeong; Kim, Eun Kyung

    2007-01-01

    The purpose of this study was to measure the absorbed dose and to calculate the effective dose for periapical radiography done by portable intraoral x-ray machines. 14 full mouth, upper posterior and lower posterior periapical radiographs were taken by wall-type 1 and portable type 3 intraoral x-ray machines. Thermoluminescent dosemeters were placed at 23 sites at the layers of the tissue-equivalent ART woman phantom for dosimetry. Average tissue absorbed dose and radiation weighted dose were calculated for each major anatomical site. Effective dose was calculated using 2005 ICRP tissue weighted factors. On 14 full mouth periapical radiographs, the effective dose for wall-type x-ray machine was 30 Sv; for portable x-ray machines were 30 Sv, 22 Sv, 36 Sv. On upper posterior radiograph, the effective dose for wall-type x-ray machine was 4 Sv; for portable x-ray machines doses were 4 Sv, 3 Sv, 5 Sv. On lower posterior radiograph, the effective dose for wall type x-ray machine was 5 Sv; for portable x-ray machines doses were 4 Sv, 4 Sv, 5 Sv. Effective doses for periapical radiographs performed by portable intraoral x-ray machines were similar to doses for periapical radiographs taken by wall type intraoral x-ray machines

  16. La influencia positiva de la CAN en la descentralización territorial colombiana

    Directory of Open Access Journals (Sweden)

    Ruth Carolina Blanco Alvarado

    2015-05-01

    Full Text Available Mediante las presentes páginas desarrollaremos la importancia del proceso andino de integración en la descentralización territorial colombiana. Para efectos de lo anterior, estableceremos, desde una perspectiva general, los principales beneficios que la descentralización territorial colombiana puede obtener en el marco de la Comunidad Andina (CAN, haciendo un análisis que descarta la perspectiva económica, núcleo rector de cualquier proceso de integración, y como consecuencia de ello, prefiriendo un análisis jurídico-político. El tipo de trabajo de investigacion empleado para las presentes páginas es sintético, esto es, trata de poner de manifiesto la totalidad del contenido de una materia o tema exponiendo sus partes principales y esenciales. A su vez, y en el marco del citada modalidad de trabajo de investigacion, las presentes páginas se circunscriben en un trabajo científico tipificado en la modalidad de “los estados de la cuestión”, esto es, en un trabajo de investigacion que recoge la situación científica de una determinada materia en un determinado momento. Adicionalmente, el método empleado para la presente investigacion es analítico, esto es, aquel que parte de lo complejo, para llegar a lo más simple.

  17. Svær asthma bronchiale

    DEFF Research Database (Denmark)

    von Bülow, Anna; Backer, Vibeke; Porsbjerg, Celeste

    2015-01-01

    Severe asthma is defined by persistent symptoms and frequent exacerbations despite intensive asthma therapy. The prevalence is estimated to be 5-10% of all asthmatics. Severe asthma is responsible for a major burden of illness including low quality of life and a disproportionate use of health......-care resources. The clinical assessment of severe asthma must include verification of the correct diagnosis, adherence to medication, excluding differential diagnosis and identification and treatment of aggravating co-morbidities and trigger factors....

  18. A study of the dose distribution in the region of the eye lens and extremities for staff working in interventional cardiology

    International Nuclear Information System (INIS)

    Domienik, J.; Brodecki, M.; Rusicka, D.

    2012-01-01

    The dose distributions at the region of eye lens and extremities of staff working in interventional cardiology were analyzed. The doses to physicians and nurses from three hospitals in Poland were measured with TL dosimeters (MCP-N) located on various places near eyebrows, on both fingers, wrists, knees and on the ankle. The procedures under investigation were coronary angiography (CA) and percutaneous coronary intervention (PCI), peacemaker and defibrillator implantations (PM/ICDs), cardiac resynchronization therapy with or without defibrillator implantations (CRT-D or CRT) and radiofrequency ablations (RFA). The study aimed at analyzing the distribution of radiation in selected anatomic regions, determining the typical locations of highest doses and estimating the dose ranges for selected types of procedures. The maximum registered doses per procedure to eye lens and ankle were 1.21 mSv and 1.46 mSv for CA PCI procedures, 0.02 mSv and 0.05 mSv for RFA and 0.13 mSv and 0.51 mSv for PM/ICDs, respectively. The maximum doses to fingers, wrists and knees were, accordingly, 2.11 mSv, 1.07 mSv and 0.77 mSv for CA PCI procedures, 0.38 mSv, 0.20 mSv and 0.04 mSv for RFA ones, 0.50 mSv, 0.25 mSv and 0.01 mSv for PM/ICDs procedures and 2.25 mSv, 1.12 mSv and 0.58 mSv for CRT and CRT-D ones. The factors which might influence the dose like utilized radiation, availability of additional protective equipment and position of the staff with respect to X-ray source were also analyzed. The annual doses for eye lens and extremities were estimated on the basis of individual annual workloads of the physicians participating in the study. The highest annual doses were revealed for physicians performing CA PCI procedures. Annual eye lens doses range up to 247 mSv indicating that the occupational limit for eye lens 150 mSv has been surpassed. In case of extremities the maximal estimated annual doses were 355 mSv, 136 mSv, 55 mSv and 328 mSv, for fingers, wrists, knees and for ankle

  19. Nutrition Following Pancreatic Surgery

    Science.gov (United States)

    ... BACK Contact Us DONATE NOW GENERAL DONATION PURPLESTRIDE Nutrition Following Pancreatic Surgery Home Facing Pancreatic Cancer Living with Pancreatic Cancer Diet and Nutrition Nutrition Following Pancreatic Surgery Ver esta página en ...

  20. Nature and distribution of feline sarcoma virus nucleotide sequences.

    Science.gov (United States)

    Frankel, A E; Gilbert, J H; Porzig, K J; Scolnick, E M; Aaronson, S A

    1979-01-01

    The genomes of three independent isolates of feline sarcoma virus (FeSV) were compared by molecular hybridization techniques. Using complementary DNAs prepared from two strains, SM- and ST-FeSV, common complementary DNA'S were selected by sequential hybridization to FeSV and feline leukemia virus RNAs. These DNAs were shown to be highly related among the three independent sarcoma virus isolates. FeSV-specific complementary DNAs were prepared by selection for hybridization by the homologous FeSV RNA and against hybridization by fline leukemia virus RNA. Sarcoma virus-specific sequences of SM-FeSV were shown to differ from those of either ST- or GA-FeSV strains, whereas ST-FeSV-specific DNA shared extensive sequence homology with GA-FeSV. By molecular hybridization, each set of FeSV-specific sequences was demonstrated to be present in normal cat cellular DNA in approximately one copy per haploid genome and was conserved throughout Felidae. In contrast, FeSV-common sequences were present in multiple DNA copies and were found only in Mediterranean cats. The present results are consistent with the concept that each FeSV strain has arisen by a mechanism involving recombination between feline leukemia virus and cat cellular DNA sequences, the latter represented within the cat genome in a manner analogous to that of a cellular gene. PMID:225544

  1. El monacato femenino en el imperio español. Monasterios, beaterios, recogimientos y colegios

    Directory of Open Access Journals (Sweden)

    Angela I. Robledo

    1996-01-01

    Full Text Available El monacato femenino en el imperio español. Monasterios, beaterios, recogimientos y colegios. Memoria del II Congreso Internacional. Manuel Ramos Medina (Coord. México: CONDUMEX, 1995. 596 páginas.

  2. Boletín bibliográfico

    Directory of Open Access Journals (Sweden)

    Facultad de Medicina Revista

    1941-07-01

    Full Text Available Parasitosis intestinales, por el doctor Fidel Fernández. Un volumen en cuarto menor de 134 páginas. Salvat Editores, S. A. Barcelona- Buenos Aires. 1940. Doctor Alvarez Herrera. - Tanatología forense. - "Manuales de Medicina Práctica" Salvat Editores, S. A. 1940. Dr. F. Gallart Monés: Ocho lesiones clínicas.- Quinta serie. Un tomo de 212 páginas y 110 fgs. Editorial Salvat. 1939. Enfermedades piogenas y parasitarias de la piel. Por el doctor José Gay Prieto catedrático numerario de Dermatología y Sifilografia de la Universidad de Granada, médico de la Lucha Oficial Antivenérea. - Un volumen de 187 pags. Salvat Editores, S. A", Barcelona, Buenos Aires, 1940. "Manuales de Medicina práctica".

  3. Dose measurements in laboratory of Physics department, University of Khartoum

    International Nuclear Information System (INIS)

    Hamid, Maria Mohammed

    1999-05-01

    Personal monitoring in University of Khartoum is being conducted using thermoluminescent dosimetry. The purpose of the study is to measure the dose of radiation in laboratory of Physics in physics department. TL phosphors LiF: Mg, Ti (card) and LiF Mg, Cu, P (GR-200) and mini-rad dosimeter are used to measure the dose in laboratory. The total dose for students form the laboratory bu using card, GR-200 and mini-rad dosimeter was found to be 2.2μ sv/year. 2.5 μ sv/year and 2.6 μ sv respectively, and for the teacher about 4.0 μ sv/year, 5.8 μ sv/year and 13.6 μ sv/year respectively, and for the dose near junk room about 3.9 μ sv/year, 2.9 μ sv/year and 2.8 μ sv/year by using card, GR-200 and mini-rad dosimeter respectively. There is just a background radiation in the main library and the applied nuclear.(Author)

  4. Kinkaleri : teater peab tekitama küsimuse, mitte andma vastuse / Marco Mazzoni ; interv. Andres Keil

    Index Scriptorium Estoniae

    Mazzoni, Marco

    2008-01-01

    1995. a. Itaalias asutatud kuuest kunstnikust - Matteo Bambi, Luca Camilletti, Massimo Conti, Marco Mazzoni, Gina Monaco ja Cristina Rizzo - koosnevast eksperimentaalteatrist Kinkaleri ja Kanuti Gildi saalis 7. ja 8. nov. esiettekandele tulevast lavastusest "Yes, Sir!"

  5. Hullud itaallased tõid dekadentsi / Eva Kübar

    Index Scriptorium Estoniae

    Kübar, Eva

    2008-01-01

    1995. a. Itaalias asutatud kuuest kunstnikust - Matteo Bambi, Luca Camilletti, Massimo Conti, Marco Mazzoni, Gina Monaco ja Cristina Rizzo - koosneva eksperimentaalgrupi Kinkaleri tantsulisest lavastusest "Yes, Sir!", mis esietendub Kanuti Gildi saalis 7. ja 8. nov

  6. Genetics Home Reference: STING-associated vasculopathy with onset in infancy

    Science.gov (United States)

    ... a site of injury or disease to fight microbial invaders and help with tissue repair. Excessive inflammation ... GINA) Turns 10 All Bulletins Features What are genome editing and CRISPR-Cas9? What is direct-to- ...

  7. Genetics Home Reference: eosinophil peroxidase deficiency

    Science.gov (United States)

    ... play a role in regulating inflammation by fighting microbial invaders. EPX gene mutations reduce or prevent eosinophil ... GINA) Turns 10 All Bulletins Features What are genome editing and CRISPR-Cas9? What is direct-to- ...

  8. Radiation risk to critical organ during brain CT

    International Nuclear Information System (INIS)

    Alaraki, N. M. E.

    2012-08-01

    Study was performed to evaluate dose to critical organ for patient undergoing CT brain in modern medical center a total of 61 patient were examined in this study. The data collected from Modern Medical Center for brain. The data collected from Modern Medical Center for brain. The eye lens dose was 31.31 mSv, skin 29.23 mSv, cranium 30.01 mSv, brain 34.50 mSv, mandible 4.39 mSv, thyroid 2.59 mSv. The organ dose value were comparable to the previous studies. (Author)

  9. Evaluation of eleven years of area monitoring for external dose rate in a deposit of radioactive waste at the ore treatment unit

    International Nuclear Information System (INIS)

    Pereira, W.S.; Oliveira, S.Q. de; Py Junior, D.A.; Silva, A.C.A.; Garcia Filho, O.

    2013-01-01

    At Ore Treatment Unit (UTM, in Portuguese) situated in Pocos de Caldas, MG, there is a deposit of about 40 tons radioactive waste produced decades ago from Santo Amaro deactivated plant (SAP) and then from NUCLEMON that processed monazite sands to extract rare earth elements. This waste contains uranium and thorium and it is stored in six hangars. This study aims to analyze the dose rates in the hangars from 2002 to 2012. Annually, two samples were obtained, for a total of 24 samples. The results showed the highest doses rates at UTM, ranging from 0.5 to 409.8 μSv h - '1 for the total set of samples. The averages extended from 0.96 μSv h -1 in hangar C-02 up to 282.64 μSv h -1 in hangar C-05. Considering each hangar separately, the results were as follows: hangar C-01 average 30.34 μSv h -1 , ranging from 6.2 to 71.7 μSv h -1 ; hangar C-02 average 0.96 μSv h -1 (min-max 0.5 to 2.51 μSv h -1 ); hangar C-05 average 282.64 μSv h -1 (min-max 3.7 to 409.8 μSv h -1 ); hangar C-06 average 188.92 μSv h -1 (min-max 1.85 to 338.0 μSv h -1 ); hangar C-07, average 172.05 μSv h -1 (min-max 1.95 to 283.0 μSv h -1 ) and hangar C-09, average 122.59 μSv h -1 (min-max 1.11 to 277.0 μSv h -1 ). ANOVA test indicated that the dose rates averages in the six hangars are different (F calc of 70.90 higher F crit of 2.28), and the Tukey test allowed to group the hangars in the following sequence: C-05> C-06 = C-07> C-09> C-01 = C-02. (author)

  10. Exploring parents' screen-viewing behaviours and sedentary time in association with their attitudes toward their young child's screen-viewing

    Directory of Open Access Journals (Sweden)

    Emma Solomon-Moore

    2017-09-01

    Full Text Available Sedentary time and screen-viewing (SV are associated with chronic disease risk in adults. Parent and child sedentary time and SV are associated. Parents influence children's SV through parenting styles and role modelling. Understanding whether parents' attitudes toward child SV are associated with their own SV and sedentary time will aid development of family interventions to reduce sedentary behaviours. Cross-sectional data with 809 parents from Bristol, UK were collected in 2012–2013 and analysed in 2016. Parental total sedentary time was derived from accelerometer data. Parents self-reported daily television viewing, use of computers, games consoles, and smartphone/tablets (none, 1–59 min, 1–2 h, >2 h and attitudes toward child SV. Adjusted linear and logistic regression models were used to examine associations, separately for weekdays and weekend days. Having negative attitudes toward child SV was associated with lower weekend sedentary time (Coeff: −6.41 [95% CI: −12.37 to −0.45] min/day. Limiting behaviours and having negative attitudes toward child SV were associated with lower weekday television viewing (OR: 0.72 [0.57–0.90] and 0.57 [0.47–0.70] respectively, weekend television viewing (0.75 [0.59–0.95] and 0.61 [0.50–0.75], and weekend computer use (0.73 [0.58–0.92] and 0.80 [0.66–0.97]. Negative attitudes were also associated with lower smartphone use on weekdays (0.70 [0.57–0.85] and weekends (0.70 [0.58–0.86]. Parent self-efficacy for limiting child SV and setting SV rules were not associated with sedentary time or SV. Reporting negative attitudes toward child SV was associated with lower accelerometer-assessed weekend total sedentary time and self-reported SV behaviours, while limiting child SV was also associated with lower self-reported SV.

  11. New acute transforming feline retovirus with fms homology specifies a C-terminally truncated version of the c-fms protein that is different from SM-feline sarcoma virus v-fms protein

    International Nuclear Information System (INIS)

    Besmer, P.; Lader, E.; George, P.C.; Bergold, P.J.; Qui, F.; Zuckerman, E.E.; Hardy, W.D.

    1986-01-01

    The HZ5-feline sarcoma virus (FeSV) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. The HZ5-FeSV transforms fibroblasts in vitro and is replication defective. A biologically active integrated HZ5-FeSV provirus was molecularly cloned from cellular DNA of HZ5-FeSV-infected FRE-3A rat cells. The HZ5-FeSV has oncogene homology with the fms sequences of the SM-FeSV. The genome organization of the 8.6-kilobase HZ5-FeSV provirus is 5' Δgag-fms-Δpol-Δenv 3'. The HZ5- and SM-FeSVs display indistinguishable in vitro transformation characteristics, and the structures of the gag-fms transforming genes in the two viruses are very similar. In the HZ5-FeSV and the SM-FeSV, identical c-fms and feline leukemia virus p10 sequences form the 5' gag-fms junction. With regard to v-fms the two viruses are homologous up to 11 amino acids before the C terminus of the SM-FeSV v-fms protein. In HZ5-FeSV a segment of 362 nucleotides then follows before the 3' recombination site with feline leukemia virus pol. The new 3' v-fms sequence encodes 27 amino acids before reaching a TGA termination signal. The relationship of this sequence with the recently characterized human c-fms sequence has been examined. The 3' HZ5-FeSV v-fms sequence is homologous with 3' c-fms sequences. A frameshift mutation (11-base-pair deletion) was found in the C-terminal fms coding sequence of the HZ5-FeSV. As a result, the HZ5-FeSV v-fms protein is predicted to be a C-terminally truncated version of c-fms. This frameshift mutation may determine the oncogenic properties of v-fms in the HZ5-FeSV

  12. New acute transforming feline retovirus with fms homology specifies a C-terminally truncated version of the c-fms protein that is different from SM-feline sarcoma virus v-fms protein

    Energy Technology Data Exchange (ETDEWEB)

    Besmer, P.; Lader, E.; George, P.C.; Bergold, P.J.; Qui, F.; Zuckerman, E.E.; Hardy, W.D.

    1986-10-01

    The HZ5-feline sarcoma virus (FeSV) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. The HZ5-FeSV transforms fibroblasts in vitro and is replication defective. A biologically active integrated HZ5-FeSV provirus was molecularly cloned from cellular DNA of HZ5-FeSV-infected FRE-3A rat cells. The HZ5-FeSV has oncogene homology with the fms sequences of the SM-FeSV. The genome organization of the 8.6-kilobase HZ5-FeSV provirus is 5' ..delta..gag-fms-..delta..pol-..delta..env 3'. The HZ5- and SM-FeSVs display indistinguishable in vitro transformation characteristics, and the structures of the gag-fms transforming genes in the two viruses are very similar. In the HZ5-FeSV and the SM-FeSV, identical c-fms and feline leukemia virus p10 sequences form the 5' gag-fms junction. With regard to v-fms the two viruses are homologous up to 11 amino acids before the C terminus of the SM-FeSV v-fms protein. In HZ5-FeSV a segment of 362 nucleotides then follows before the 3' recombination site with feline leukemia virus pol. The new 3' v-fms sequence encodes 27 amino acids before reaching a TGA termination signal. The relationship of this sequence with the recently characterized human c-fms sequence has been examined. The 3' HZ5-FeSV v-fms sequence is homologous with 3' c-fms sequences. A frameshift mutation (11-base-pair deletion) was found in the C-terminal fms coding sequence of the HZ5-FeSV. As a result, the HZ5-FeSV v-fms protein is predicted to be a C-terminally truncated version of c-fms. This frameshift mutation may determine the oncogenic properties of v-fms in the HZ5-FeSV.

  13. Evaluation of eleven years of area monitoring for external dose rate in a deposit of radioactive waste at the ore treatment unit

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, W.S.; Oliveira, S.Q. de; Py Junior, D.A.; Silva, A.C.A.; Garcia Filho, O., E-mail: pereiraws@gmail.com [Industrias Nucleares do Brasil (INB), Pocos de Caldas, MG (Brazil). Unidade de Tratamento de Minerio. Grupo Multidisciplinar de Radioprotecao; Kelecom, A., E-mail: akelecom@id.uff.br [Universidade Federal Fluminense (LARARA-PLS/GETA/UFF), Niteroi, RJ, (Brazil). Lab. de Radiobiologia e Radiometria. Grupo de Estudos em Temas Ambientais; Pereira, J.R.S., E-mail: pereirarsj@gmail.com [Universidade Federal de Alfenas (UNIFAL), Pocos de Caldas, MG (Brazil)

    2013-07-01

    At Ore Treatment Unit (UTM, in Portuguese) situated in Pocos de Caldas, MG, there is a deposit of about 40 tons radioactive waste produced decades ago from Santo Amaro deactivated plant (SAP) and then from NUCLEMON that processed monazite sands to extract rare earth elements. This waste contains uranium and thorium and it is stored in six hangars. This study aims to analyze the dose rates in the hangars from 2002 to 2012. Annually, two samples were obtained, for a total of 24 samples. The results showed the highest doses rates at UTM, ranging from 0.5 to 409.8 μSv h{sup -}'1 for the total set of samples. The averages extended from 0.96 μSv h{sup -1} in hangar C-02 up to 282.64 μSv h{sup -1} in hangar C-05. Considering each hangar separately, the results were as follows: hangar C-01 average 30.34 μSv h{sup -1}, ranging from 6.2 to 71.7 μSv h{sup -1}; hangar C-02 average 0.96 μSv h{sup -1} (min-max 0.5 to 2.51 μSv h{sup -1}); hangar C-05 average 282.64 μSv h{sup -1} (min-max 3.7 to 409.8 μSv h{sup -1}); hangar C-06 average 188.92 μSv h{sup -1} (min-max 1.85 to 338.0 μSv h{sup -1}); hangar C-07, average 172.05 μSv h{sup -1} (min-max 1.95 to 283.0 μSv h{sup -1}) and hangar C-09, average 122.59 μSv h{sup -1} (min-max 1.11 to 277.0 μSv h{sup -1}). ANOVA test indicated that the dose rates averages in the six hangars are different (F{sub calc} of 70.90 higher F{sub crit} of 2.28), and the Tukey test allowed to group the hangars in the following sequence: C-05> C-06 = C-07> C-09> C-01 = C-02. (author)

  14. Factors associated with sexual violence against men who have sex with men and transgendered individuals in Karnataka, India.

    Directory of Open Access Journals (Sweden)

    Souradet Y Shaw

    Full Text Available There is a lack of information on sexual violence (SV among men who have sex with men and transgendered individuals (MSM-T in southern India. As SV has been associated with HIV vulnerability, this study examined health related behaviours and practices associated with SV among MSM-T.Data were from cross-sectional surveys from four districts in Karnataka, India.Multivariable logistic regression models were constructed to examine factors related to SV. Multivariable negative binomial regression models examined the association between physician visits and SV.A total of 543 MSM-T were included in the study. Prevalence of SV was 18% in the past year. HIV prevalence among those reporting SV was 20%, compared to 12% among those not reporting SV (p = .104. In multivariable models, and among sex workers, those reporting SV were more likely to report anal sex with 5+ casual sex partners in the past week (AOR: 4.1; 95%CI: 1.2-14.3, p = .029. Increased physician visits among those reporting SV was reported only for those involved in sex work (ARR: 1.7; 95%CI: 1.1-2.7, p = .012.These results demonstrate high levels of SV among MSM-T populations, highlighting the importance of integrating interventions to reduce violence as part of HIV prevention programs and health services.

  15. Stroke Volume estimation using aortic pressure measurements and aortic cross sectional area: Proof of concept.

    Science.gov (United States)

    Kamoi, S; Pretty, C G; Chiew, Y S; Pironet, A; Davidson, S; Desaive, T; Shaw, G M; Chase, J G

    2015-08-01

    Accurate Stroke Volume (SV) monitoring is essential for patient with cardiovascular dysfunction patients. However, direct SV measurements are not clinically feasible due to the highly invasive nature of measurement devices. Current devices for indirect monitoring of SV are shown to be inaccurate during sudden hemodynamic changes. This paper presents a novel SV estimation using readily available aortic pressure measurements and aortic cross sectional area, using data from a porcine experiment where medical interventions such as fluid replacement, dobutamine infusions, and recruitment maneuvers induced SV changes in a pig with circulatory shock. Measurement of left ventricular volume, proximal aortic pressure, and descending aortic pressure waveforms were made simultaneously during the experiment. From measured data, proximal aortic pressure was separated into reservoir and excess pressures. Beat-to-beat aortic characteristic impedance values were calculated using both aortic pressure measurements and an estimate of the aortic cross sectional area. SV was estimated using the calculated aortic characteristic impedance and excess component of the proximal aorta. The median difference between directly measured SV and estimated SV was -1.4ml with 95% limit of agreement +/- 6.6ml. This method demonstrates that SV can be accurately captured beat-to-beat during sudden changes in hemodynamic state. This novel SV estimation could enable improved cardiac and circulatory treatment in the critical care environment by titrating treatment to the effect on SV.

  16. Looking Ahead Toward Community-Level Strategies to Prevent Sexual Violence

    Science.gov (United States)

    DeGue, Sarah; Holt, Melissa K.; Massetti, Greta M.; Matjasko, Jennifer L.; Tharp, Andra Teten; Valle, Linda Anne

    2018-01-01

    The Division of Violence Prevention within CDC’s National Center for Injury Prevention and Control recently undertook a systematic review of primary prevention strategies for sexual violence (SV) perpetration. This review identified the lack of community-level strategies to prevent SV as a critical gap in the literature. Community-level strategies function by modifying the characteristics of settings (e.g., schools, workplaces, neighborhoods) that increase the risk for violence victimization and perpetration. Identification of evidence-based strategies at the community level would allow implementation of ecologic approaches to SV prevention with a greater potential for reducing the prevalence of SV perpetration. The field will face several challenges in identifying and evaluating the effectiveness of promising community-level strategies to prevent SV. These challenges include limited knowledge of community-level and societal-level risk factors for SV, a lack of theoretical or empirical guidance in the SV literature for identification of promising community-level approaches, and challenges in evaluating SV outcomes at the community level. Recognition of these challenges should guide future research and foster dialogue within the SV prevention field. The development and evaluation of community-level approaches to SV prevention represent a vital and logical next step toward the implementation of effective, multilevel prevention efforts and a population-level reduction in the prevalence of SV. PMID:22185587

  17. Enhancement of SV40 transformation by treatment of C3H2K cells with uv light and caffeine. I. Combined effect of uv light and caffeine

    International Nuclear Information System (INIS)

    Ide, T.; Anzai, K.; Andoh, T.

    1975-01-01

    Treatment of cultured mouse cells, C3H2K, with uv light and/or caffeine enhanced the frequency of SV40-induced transformation. This enhancement depends upon the doses of uv and caffeine and the mode of combination of these agents. Irradiation of cells with increasing doses of uv just before infection resulted in approximately 2-fold enhancement of the transformation frequency up to a dose of 90 ergs/mm 2 and 3.3-fold at 150 ergs/mm 2 . Addition of 1 mM caffeine to the medium for 4 days subsequent to infection brought about a 2-fold enhancement. When cells were irradiated and treated with 1 mM caffeine, the enhancement was approximately 4-fold up to a uv dose of 90 ergs/mm 2 and 5.9-fold at 150 ergs/mm 2 . When 0.1 to 4 mM caffeine was added for 4 days postinfection, the absolute number of transformations increased, and an enhancement ratio of 1.3 to 6.8 resulted. After the addition of the same increasing doses of caffeine to uv-irradiated cells (75 ergs/mm 2 ), the enhancement of transformation frequency was even higher ranging 2.0 to 13.3. The transformation frequencies thus obtained by the double treatment were always higher than those predicted if uv and caffeine acted additively. The transformation frequency was little affected by the addition of dibutyrylcyclic AMP and theophylline

  18. Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain

    International Nuclear Information System (INIS)

    Meinke, Gretchen; Phelan, Paul; Fradet-Turcotte, Amélie; Archambault, Jacques; Bullock, Peter A.

    2011-01-01

    With the aim of forming the ‘lock-washer’ conformation of the origin-binding domain of SV40 large T antigen in solution, using structure-based analysis an intermolecular disulfide bridge was engineered into the origin-binding domain to generate higher order oligomers in solution. The 1.7 Å resolution structure shows that the mutant forms a spiral in the crystal and has the de novo disulfide bond at the protein interface, although structural rearrangements at the interface are observed relative to the wild type. The modular multifunctional protein large T antigen (T-ag) from simian virus 40 orchestrates many of the events needed for replication of the viral double-stranded DNA genome. This protein assembles into single and double hexamers on specific DNA sequences located at the origin of replication. This complicated process begins when the origin-binding domain of large T antigen (T-ag ODB) binds the GAGGC sequences in the central region (site II) of the viral origin of replication. While many of the functions of purified T-ag OBD can be studied in isolation, it is primarily monomeric in solution and cannot assemble into hexamers. To overcome this limitation, the possibility of engineering intermolecular disulfide bonds in the origin-binding domain which could oligomerize in solution was investigated. A recent crystal structure of the wild-type T-ag OBD showed that this domain forms a left-handed spiral in the crystal with six subunits per turn. Therefore, we analyzed the protein interface of this structure and identified two residues that could potentially support an intermolecular disulfide bond if changed to cysteines. SDS–PAGE analysis established that the mutant T-ag OBD formed higher oligomeric products in a redox-dependent manner. In addition, the 1.7 Å resolution crystal structure of the engineered disulfide-linked T-ag OBD is reported, which establishes that oligomerization took place in the expected manner

  19. Estimates of effective dose in adult CT examinations

    International Nuclear Information System (INIS)

    Mohamed, Mustafa Awad Elhaj.

    2015-12-01

    The goal of study was to estimate effective dose (E) in adult CT examinations for Toshiba X64 slice using CT. Exp version 2.5 software in Sudan. Using of CT in medical diagnosis delivers radiation doses to patients that are higher than those from other radiological procedures. lack of optimized protocols could be an additional source of increased dose in developing countries. In order to achieve these objectives, data of CT-scanner has been collected from three hospitals ( ANH, ZSH and MMH). Data collected included equipment information and scan parameters for individual patients, who were used to asses. 300 adult patients underwent head, chest, abdomen-pelvis and peivis CT examinations. The CT1_w , CTD1_vol, DLP, patient effective dos and organ doses were estimated, using CT exposure parameters and CT Exp version 2.5 software. A large variation of mean effective dose and organ doses among hospitals was observed for similar CT examinations. These variations largely originated from different CT scanning protocols used in different hospitals and scan length. The mean effective dose in this study in the Brain, PNS, Chest, pulmonary, Abdomen-pelvis, Pelvis, KUB and CTU were 3.2 mSv, 2.6 mSv, 18.9 mSv 17.6 mSv 27.1 mSv, 11.2 mSv, 9.6 mSv and 23.7 mSv respectively, and organ equivalent, doses presented in this study in this study for the eye lens (for head), lungs and thymus ( for chest) , liver, kidney and small intest ( for abdomen t-pelvis), bladder, uterus and gonads ( for pelvis), were 62.9 mSv, 39.5 mSv, 34.1 mSv, 53.9 mSv, 52.6 mSv, 58.1 mSv, 37 mSv, and 34.6 mSv, respectively. These values were mostly comparable to and slightly higher than the values of effective doses reported from similar studies the United Kingdom, Tanzania, Australia, Canada and Sudan. It was concluded that patient effective dose and organ doses could be substantially minimized through careful selection of scanning parameters based on clinical indications of study, patient size, and body

  20. Alteraciones metabólicas en individuos mexicanos con hiperlipidemia familiar combinada y las variantes del polimorfismo de nucleótido único rs1424032 /

    OpenAIRE

    Almeda Valdes, Paloma

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Medicas, presenta Paloma Almeda Valdes ; asesor Carlos Aguilar Salinas68 páginas : ilustracionesDoctorado en Ciencias Medicas UNAM, Facultad de Medicina, 2012

  1. Influence of nuclear reactor accident at Chernobyl' on the environmental radioactivity in Toyama

    International Nuclear Information System (INIS)

    Morita, Miyuki; Shoji, Miki; Honda, Takashi; Sakanoue, Masanobu.

    1987-01-01

    The environmental radioactivity caused by the reactor accident at Chernobyl' was investigated from May 7 to May 31 of 1986 in Toyama. Measurement of radioactivities in airborne particles, rain water, drinking water, milk, and mugwort are carried out by gamma-ray spectrometry (pure Ge detector; ORTEC GMX-23195). Ten different nuclides ( 103 Ru, 106 Ru, 131 I, 132 Te-I, 134 Cs, 136 Cs, 137 Cs, 140 Ba-La) are identified from samples of airborne particles. In the air samples, a maximum radioactivity concentration of each nuclide is observed on 13th May 1986. The time of the reactor shut-down and the flux of thermal neutron at the reactor were calculated from 131 I/ 132 I and 137 Cs/ 134 Cs ratio. The exposure dose in Toyama by this accident is given as follows: internal exposure; [thyroid] adult-59 μSv, child-140 μSv, baby-130 μSv, [total body] adult-0.2 μSv, child, baby-0.4 μSv, external exposure; 7 μSv, effective dose equivalent; adult-9 μSv, child-12 Sv, baby-11 μSv. (author)

  2. Collective effective dose in Europe from x-ray and nuclear medicine procedures

    International Nuclear Information System (INIS)

    Bly, R.; Jaervinen, H.; Jahnen, A.; Olerud, H.; Vassileva, J.; Vogiatzi, S.

    2015-01-01

    Population doses from radiodiagnostic (X-ray and nuclear medicine) procedures in Europe were estimated based on data collected from 36 European countries. For X-ray procedures in EU and EFTA countries (except Liechtenstein) the collective effective dose is 547 500 man Sv, resulting in a mean effective dose of 1.06 mSv per caput. For all European countries included in the survey the collective effective dose is 605 000 man Sv, resulting in a mean effective dose of 1.05 mSv per caput. For nuclear medicine procedures in EU countries and EFTA (except Liechtenstein) countries the collective effective dose is 30 700 man Sv, resulting in a mean effective dose of 0.06 mSv per caput. For all European countries included in the survey the collective effective dose is 31 100 man Sv, resulting in a mean effective dose of 0.05 mSv per caput. (authors)

  3. As hemerotecas digitais discursivizadas na web: heterogeneidade discursiva e formação imaginária na rede

    Directory of Open Access Journals (Sweden)

    Francis Lampoglia

    2011-10-01

    Full Text Available Este trabalho estuda o funcionamento discursivo de duas páginas da web, sendo uma do Arquivo Público do Estado, referente à hemeroteca do jornal Última Hora, e outra referente ao site do jornal O Estado de S. Paulo, mais precisamente à seção "Especiais", intitulado "Nas páginas do Estadão, a luta contra a censura". Sob a orientação da Análise do Discurso de matriz francesa, fundada por Michel Pêcheux, com o apoio dos estudos sobre o dialogismo de Mikhail Bakhtin e das discussões sobre o virtual de Pierre Lévy, esse trabalho visa compreender o modo como são produzidos efeitos de sentido sobre o entrelaçamento de vozes que compõem as páginas web analisadas, bem como analisar qual a formação imaginária que tais sites constroem de si e de seus leitores através do discurso eletrônico. Inseridos em um ambiente eletrônico, os sites que abrigam acervos de jornais e revistas, além de constituir um arquivo sob uma perspectiva histórica, também tentam construir uma imagem de si e/ou do jornal que abrigam. Entendendo que a rede mundial de computadores é uma realidade, destacamos a importância de se estudar o discurso presente nos sites como forma de romper com a suposta neutralidade da imprensa e da web.

  4. Genetic Diversity of Pinus nigra Arn. Populations in Southern Spain and Northern Morocco Revealed By Inter-Simple Sequence Repeat Profiles

    Directory of Open Access Journals (Sweden)

    Oussama Ahrazem

    2012-05-01

    Full Text Available Eight Pinus nigra Arn. populations from Southern Spain and Northern Morocco were examined using inter-simple sequence repeat markers to characterize the genetic variability amongst populations. Pair-wise population genetic distance ranged from 0.031 to 0.283, with a mean of 0.150 between populations. The highest inter-population average distance was between PaCU from Cuenca and YeCA from Cazorla, while the lowest distance was between TaMO from Morocco and MA Sierra Mágina populations. Analysis of molecular variance (AMOVA and Nei’s genetic diversity analyses revealed higher genetic variation within the same population than among different populations. Genetic differentiation (Gst was 0.233. Cuenca showed the highest Nei’s genetic diversity followed by the Moroccan region, Sierra Mágina, and Cazorla region. However, clustering of populations was not in accordance with their geographical locations. Principal component analysis showed the presence of two major groups—Group 1 contained all populations from Cuenca while Group 2 contained populations from Cazorla, Sierra Mágina and Morocco—while Bayesian analysis revealed the presence of three clusters. The low genetic diversity observed in PaCU and YeCA is probably a consequence of inappropriate management since no estimation of genetic variability was performed before the silvicultural treatments. Data indicates that the inter-simple sequence repeat (ISSR method is sufficiently informative and powerful to assess genetic variability among populations of P. nigra.

  5. Combining modelling and mutagenesis studies of synaptic vesicle protein 2A to identify a series of residues involved in racetam binding.

    Science.gov (United States)

    Shi, Jiye; Anderson, Dina; Lynch, Berkley A; Castaigne, Jean-Gabriel; Foerch, Patrik; Lebon, Florence

    2011-10-01

    LEV (levetiracetam), an antiepileptic drug which possesses a unique profile in animal models of seizure and epilepsy, has as its unique binding site in brain, SV2A (synaptic vesicle protein 2A). Previous studies have used a chimaeric and site-specific mutagenesis approach to identify three residues in the putative tenth transmembrane helix of SV2A that, when mutated, alter binding of LEV and related racetam derivatives to SV2A. In the present paper, we report a combined modelling and mutagenesis study that successfully identifies another 11 residues in SV2A that appear to be involved in ligand binding. Sequence analysis and modelling of SV2A suggested residues equivalent to critical functional residues of other MFS (major facilitator superfamily) transporters. Alanine scanning of these and other SV2A residues resulted in the identification of residues affecting racetam binding, including Ile273 which differentiated between racetam analogues, when mutated to alanine. Integrating mutagenesis results with docking analysis led to the construction of a mutant in which six SV2A residues were replaced with corresponding SV2B residues. This mutant showed racetam ligand-binding affinity intermediate to the affinities observed for SV2A and SV2B.

  6. The Study of External Radiation Dose for Radiation Worker at PRSG-BATAN Serpong

    International Nuclear Information System (INIS)

    Sunarningsih; Mashudi; A Lilik W; Yosep S

    2012-01-01

    The study of External radiation dose for radiation worker at PRSG-BATAN Serpong has been carried out. The sample is taken from the System Reactor division (BSR), Operation Reactor division, (BOR) Safety division UPN, UJM and head of PRSG by setting Thermoluminescence Dosemeter (TLD) on the chest, then is detected by a tool TLD reader model 6600. The aim of this study is to evaluate the occupational exposure dose that has been accepted by the radiation worker for the last five years. The result in average doses at BSR is 0,99 mSv, BOR is 3,27 mSv, at BK is 0,69 mSv and UPN + UJM + head of PRSG is 0,03 mSv. The result highest doses at BSR is 6,58 mSv, BOR is 28,94 mSv, BK is 4,24 mSv, and UPN UJM Head of PRSG is 0,52 mSv. Dose interval radiation worker at PRSG BATAN ttd - 28,98 mSv. To overall the external personal dose acceptant for radiation worker at PRSG BATAN one below maximum permissible dose acceptant that allowed by BAPETEN, that is 20 mSv in average every year during five years. (author)

  7. Oxidative shift in tissue redox potential increases beat-to-beat variability of action potential duration.

    Science.gov (United States)

    Kistamás, Kornél; Hegyi, Bence; Váczi, Krisztina; Horváth, Balázs; Bányász, Tamás; Magyar, János; Szentandrássy, Norbert; Nánási, Péter P

    2015-07-01

    Profound changes in tissue redox potential occur in the heart under conditions of oxidative stress frequently associated with cardiac arrhythmias. Since beat-to-beat variability (short term variability, SV) of action potential duration (APD) is a good indicator of arrhythmia incidence, the aim of this work was to study the influence of redox changes on SV in isolated canine ventricular cardiomyocytes using a conventional microelectrode technique. The redox potential was shifted toward a reduced state using a reductive cocktail (containing dithiothreitol, glutathione, and ascorbic acid) while oxidative changes were initiated by superfusion with H2O2. Redox effects were evaluated as changes in "relative SV" determined by comparing SV changes with the concomitant APD changes. Exposure of myocytes to the reductive cocktail decreased SV significantly without any detectable effect on APD. Application of H2O2 increased both SV and APD, but the enhancement of SV was the greater, so relative SV increased. Longer exposure to H2O2 resulted in the development of early afterdepolarizations accompanied by tremendously increased SV. Pretreatment with the reductive cocktail prevented both elevation in relative SV and the development of afterdepolarizations. The results suggest that the increased beat-to-beat variability during an oxidative stress contributes to the generation of cardiac arrhythmias.

  8. Jornalismo e convergência: reflexões sobre o futuro do jornalismo (M. Engel Bronosky & J. Maurício de Carvalho)

    OpenAIRE

    André Luís Lourenço

    2017-01-01

    Jornalismo e convergência: reflexões sobre o futuro do jornalismo Organizadores: Marcelo Engel Bronosky e Juliano Maurício de Carvalho Editorial: Editora Cultura Acadêmica Ano: 2014 Páginas: 280 ISBN: 978-85-7983-551-3

  9. Información general sobre la preparación y la respuesta frente a desastres naturales

    Science.gov (United States)

    Esta página brinda información general sobre la manera en que se puede preparar para enfrentar desastres naturales potenciales y las acciones a tomar después de dichos desastres naturales y otras emergencias ambientales.

  10. Global strategy for the diagnosis and management of asthma in children 5 years and younger

    DEFF Research Database (Denmark)

    Hurd, Suzanne S; Lemanske, Robert F; Becker, Allan

    2011-01-01

    in this age group. For this reason, to aid in the diagnosis of asthma in young children, a symptoms-only descriptive approach that includes the definition of various wheezing phenotypes has been recommended. In 1993, the Global Initiative for Asthma (GINA) was implemented to develop a network of individuals...... and our ability to manage and control it effectively. However, in children 5 years and younger, the clinical symptoms of asthma are variable and non-specific. Furthermore, neither airflow limitation nor airway inflammation, the main pathologic hallmarks of the condition, can be assessed routinely......, organizations, and public health officials to disseminate information about the care of patients with asthma while at the same time assuring a mechanism to incorporate the results of scientific investigations into asthma care. Since then, GINA has developed and regularly revised a Global Strategy for Asthma...

  11. A micro-controller based palm-size radiation monitor

    International Nuclear Information System (INIS)

    Bhingare, R.R.; Bajaj, K.C.; Kannan, S.

    2001-01-01

    A micro-controller based, palm-size radiation monitor, PALMRAD, using a silicon P-N junction diode as a detector has been developed. It is useful for radiation protection monitoring during radiation emergency as well as radioactive source loading operations. Some of the features of PALMRAD developed are the use of a semiconductor diode as the detector, simultaneous display of integrated dose and dose rate on a 16-digit alpha numeric LCD display, measurable integrated dose range from 1 μSv to 5000 μSv and dose rate range from 1 mSv/h to 1,000 mSv/h, RS 232C serial interface for connection to a Personal Computer,-storage of integrated dose and dose rate readings, recall of stored readings on LCD display, presentable integrated dose alarm from 1 μSv to 5000 μSv and dose rate from 1 mSv/h to 1,000 mSv/h, battery status and memory status check during measurement, LCD display with LED back-lighting, etc. (author)

  12. Safeguards of Neurotransmission: Endocytic Adaptors as Regulators of Synaptic Vesicle Composition and Function

    Directory of Open Access Journals (Sweden)

    Natalie Kaempf

    2017-10-01

    Full Text Available Communication between neurons relies on neurotransmitters which are released from synaptic vesicles (SVs upon Ca2+ stimuli. To efficiently load neurotransmitters, sense the rise in intracellular Ca2+ and fuse with the presynaptic membrane, SVs need to be equipped with a stringently controlled set of transmembrane proteins. In fact, changes in SV protein composition quickly compromise neurotransmission and most prominently give rise to epileptic seizures. During exocytosis SVs fully collapse into the presynaptic membrane and consequently have to be replenished to sustain neurotransmission. Therefore, surface-stranded SV proteins have to be efficiently retrieved post-fusion to be used for the generation of a new set of fully functional SVs, a process in which dedicated endocytic sorting adaptors play a crucial role. The question of how the precise reformation of SVs is achieved is intimately linked to how SV membranes are retrieved. For a long time both processes were believed to be two sides of the same coin since Clathrin-mediated endocytosis (CME, the proposed predominant SV recycling mode, will jointly retrieve SV membranes and proteins. However, with the recent proposal of Clathrin-independent SV recycling pathways SV membrane retrieval and SV reformation turn into separable events. This review highlights the progress made in unraveling the molecular mechanisms mediating the high-fidelity retrieval of SV proteins and discusses how the gathered knowledge about SV protein recycling fits in with the new notions of SV membrane endocytosis.

  13. Basics of LASIK Eye Surgery

    Science.gov (United States)

    ... Vea esta página en español The Basics of LASIK Eye Surgery Share This Page Facebook Twitter Linked- ... Surgery Surgical Alternatives to LASIK For More Information  LASIK Basics If you wear glasses or contact lenses, ...

  14. Ciclo cósmico del agua

    OpenAIRE

    Martínez-Frías, J.

    2008-01-01

    1 página.-- Trabajo presentado en la Exposición Internacional "Agua y Desarrollo Sostenible" celebrada en Zaragoza del 14 de Junio al 14 de septiembre del 2008 (Pabellón: Torre del Agua. Agua para la vida).

  15. Comparative human cellular radiosensitivity: Pt. 1

    International Nuclear Information System (INIS)

    Arlett, C.F.; Green, M.H.L.; Priestley, A.; Harcourt, S.A.; Mayne, L.V.

    1988-01-01

    The authors compared cell killing following 60 Co gamma irradiation in 22 primary human fibroblast strains, nine SV40-immortalized human fibroblast lines and seven SV40-transformed pre-crisis human fibroblast cultures from normal individuals, from ataxia-telangiectasia (A-T) patients and from A-T heterozygotes. They confirmed the greater sensitivity of A-T derived cells to gamma radiation. The distinction between A-T and normal cells is maintained in cells immortalized by SV40-virus but immortal cells are more gamma radiation resistant than corresponding primary fibroblasts. Cells transformed by plasmids (pSV3gpt and pSV3neo) expressing SV40 T-antigen, both pre- and post-crisis, show this increased resistance, indicating that expression of SV40 T-antigen, rather than immortalization per se is responsible for the change. (author)

  16. Optimal contouring of seminal vesicle for definitive radiotherapy of localized prostate cancer: comparison between EORTC prostate cancer radiotherapy guideline, RTOG0815 protocol and actual anatomy

    International Nuclear Information System (INIS)

    Qi, Xin; Gao, Xian-Shu; Asaumi, Junichi; Zhang, Min; Li, Hong-Zhen; Ma, Ming-Wei; Zhao, Bo; Li, Fei-Yu; Wang, Dian

    2014-01-01

    Intermediate- to-high-risk prostate cancer can locally invade seminal vesicle (SV). It is recommended that anatomic proximal 1-cm to 2-cm SV be included in the clinical target volume (CTV) for definitive radiotherapy based on pathology studies. However, it remains unclear whether the pathology indicated SV extent is included into the CTV defined by current guidelines. The purpose of this study is to compare the volume of proximal SV included in CTV defined by EORTC prostate cancer radiotherapy guideline and RTOG0815 protocol with the actual anatomic volume. Radiotherapy planning CT images from 114 patients with intermediate- (36.8%) or high-risk (63.2%) prostate cancer were reconstructed with 1-mm-thick sections. The starting and ending points of SV and the cross sections of SV at 1-cm and 2-cm from the starting point were determined using 3D-view. Maximum (D 1H , D 2H ) and minimum (D 1L , D 2L ) vertical distance from these cross sections to the starting point were measured. Then, CTV of proximal SV defined by actual anatomy, EORTC guideline and RTOG0815 protocol were contoured and compared (paired t test). Median length of D 1H , D 1L , D 2H and D 2L was 10.8 mm, 2.1 mm, 17.6 mm and 8.8 mm (95th percentile: 13.5mm, 5.0mm, 21.5mm and 13.5mm, respectively). For intermediate-risk patients, the proximal 1-cm SV CTV defined by EORTC guideline and RTOG0815 protocol inadequately included the anatomic proximal 1-cm SV in 62.3% (71/114) and 71.0% (81/114) cases, respectively. While for high-risk patients, the proximal 2-cm SV CTV defined by EORTC guideline inadequately included the anatomic proximal 2-cm SV in 17.5% (20/114) cases. SV involvement indicated by pathology studies was not completely included in the CTV defined by current guidelines. Delineation of proximal 1.4 cm and 2.2 cm SV in axial plane may be adequate to include the anatomic proximal 1-cm and 2-cm SV. However, part of SV may be over-contoured

  17. Correlates of disclosure of sexual violence among Kenyan youth.

    Science.gov (United States)

    Boudreau, Courtney L; Kress, Howard; Rochat, Roger W; Yount, Kathryn M

    2018-05-01

    Sexual violence (SV) against children is a global health and human rights issue that can have short and long-term consequences for health and wellbeing. Disclosing SV increases the likelihood that children can access health and protective services and receive psychosocial support. Research in high-income countries has found that child SV survivors are more likely to disclose when they are girls/women, experience fewer SV events, and experience SV perpetrated by a stranger. No studies have examined correlates of SV disclosure in Kenya. The objective of this research was to assess the correlates of disclosing SV among Kenyan youth ages 13-24 who reported an SV experience before age 18. In 2010, the Kenya Ministry of Gender, Children and Social Development, the U.S. Centers for Disease Control and Prevention's (CDC) Division of Violence Prevention, the UNICEF Kenya Country Office, and the Kenya National Bureau of Statistics (KNBS) conducted a national survey of violence against children. These data were used to conduct weighted logistic regression analyses to determine which factors were correlated with reporting SV disclosure. Among the 27.8% of girls/women and 14.5% of boys/men who reported SV before age 18, 44.6% of girls/women and 28.2% of boys/men reported to have disclosed the experience. In weighted logistic regression analysis, the odds of disclosure were lower among survivors who were boys/men and among survivors who reported more SV events, and higher when any perpetrator was a family member. More context-specific research on SV disclosure among young people is needed globally. Copyright © 2018 Elsevier Ltd. All rights reserved.

  18. Radiation dose of staff during positron emission tomography study

    Energy Technology Data Exchange (ETDEWEB)

    Hachiya, Takenori; Shoji, Yasuaki; Hagami, Eiichi; Aizawa, Yasuo; Onodera, Hiroshi; Kan, Mikio; Sasaki, Nobuo; Toyoshima, Eiji; Sugawara, Shigeki

    1989-04-01

    In this paper, the radiation dose of the PET staff including blood sampling operators (medical doctors), PET operators (radio technologists), nurses, blood analysts (medical technologists), radiochemists and cyclotron operators in the cyclotron-PET unit was evaluated. The dose was measured using the film-badges for whole body and TLD ring-badges for fingers. These data were obtained from June, 1986 to May, 1987. In the average dose per month, those with the highest dose were radiochemists. The whole body dose was 1.08 mSv (108 mrem) for /gamma/-ray, 520 /mu/Sv (52 mrem) for /beta/-ray and the dose for fingers, 7.6mSv (760 mrem). The total dose per year both for /gamma/ and /beta/-ray was 19.2 mSv (1.92 rem) for whole body and 91.2 mSv (9.12 rem) for fingers. The staff with the lowest whole body dose for /gamma/-ray per month was blood analysts whose dose was 30 /mu/Sv (3 mrem). For the staff consisting of doctors, radiotechnologists and nurses, the whole body dose per month was 70-170/mu/Sv (7-17 mrem) for /gamma/-ray, 20 /mu/Sv (2 mrem) for /beta/-ray and the dose for fingers per month was 210-540 /mu/Sv (21-54 mrem). For cyclotron operators, the dose was 90 /mu/Sv (9 mrem) for /gamma/-ray, 30 /mu/Sv (3 mrem) for /beta/-ray and the dose for fingers was 160 /mu/Sv (16 mrem). For cyclotron operators and radiochemists, the whole body dose for the neutron ray was measured by solid state nuclear track detector (SSNTD), but the dose was found to be negligible. (author).

  19. Sobre coisas de outros tempos: rastros biográficos nas crônicas de Cecília Meireles na Página de Educação - About things from other times: biographic traces of Cecília Meireles stories in the Education Page

    Directory of Open Access Journals (Sweden)

    Ana Chrystina Venancio Mignot

    2011-03-01

    Full Text Available Resumo Adentrar  pela urdidura  narrativa  da  crônica  “A  escola  atraente” permite discutir a relação entre objetos  escolares e memória e, ao mesmo tempo, examinar o papel desempenhado por Cecília Meireles no debate  educacional dos anos 30, do século passado, debate este marcado por uma  nova  sensibilidade pela infância e  que  conferiu identidade a uma geração de intelectuais que apostou na constituição do  campo educacional. Investigar o horizonte de  intervenção  da cronista permitiu uma aproximação com um ângulo menos estudado da biografia intelectual da poeta, jornalista e educadora. Palavras-chave: Cecília Meireles; Escola Nova;  biografia;  cultura material da escola.   ABOUT  THINGS  FROM OTHER TIMES: BIOGRAPHIC TRACES OF CECILIA MEIRELES’ STORIES  IN THE EDUCATION PAGE Abstract The  plot  of the story “A escola atraente”  (The  attractive  school opens a discussion about  the  relation  between  school objects and memory and, at  the same  time,  reveals  the role played by Cecilia Meireles in the education debates of the 1930’s, which were marked by a  new understanding  of  childhood  by a  whole  generation  of intellectuals that  had placed their bets in  the constitution  of the education field. The investigation of Meireles' range of intervention resulted  in  approaching a less known aspect of her intellectual biography as a poet, journalist and education scholar. Keywords: Cecilia Meireles; New School; biography; school material culture.     SOBRE COSAS DE OTROS TIEMPOS: RASTROS BIOGRÁFICOS EN LAS CRÓNICAS DE CECÍLIA MEIRELES EN LA “PÁGINA DE EDUCACIÓN” Resumen Adentrar  por  la  urdidura  narrativa  de  la  crónica  “La   escuela atractiva”   permite  discutir  la  relación  entre   objetos  escolares  y memoria y, al mismo tiempo, examinar el rol de Cecília Meireles en el debate  educacional de los años 30

  20. Occupational exposure to eye-lenses and extremities in interventional cardiology

    International Nuclear Information System (INIS)

    Jankowski, Jerzy; Brodecki, Marcin; Kosmider, Maciej; Tylkowski, Michal

    2008-01-01

    Full text: Despite of very fast developments in the field of Interventional Cardiology, the level of doses received by medical staff is still substantial and therefore must be routinely controlled. The annual dose limits established by International Commission on Radiological Protection are monitored by the personal dosimeters worn under the lead apron. In this method the information about doses on uncovered body parts can be only approximated. Values obtained by means of ring or wrist dosimetry are also insufficient to provide basis for conclusions on the extremities doses received by medical staff. The main purpose of this study was to investigate radiation doses absorbed for eye-lenses and extremities to personnel participating in cardiac procedures. The doses were measured using highly sensitive thermoluminescent dosimeters (LiF: Mg, Cu, P), calibrated in Secondary Standard Dosimetry Laboratory for X-ray beam typical of cardiac procedures. TLDs were placed in three locations: in a special band on eyebrow ridge, on a left ankle and on a left hand (finger's ring). Considerable amount of detectors used allowed determining the actual distribution of doses. Mean equivalent doses per procedure for Coronary Angiography (CAG) and Coronary Angiography with Percutaneous Transluminal Coronary Angioplasty (CAG+PTCA) were investigated. For CAG these values amounted to: on left eyebrow ridge from 80 μSv to 140 μSv, on right eyebrow ridge from 30 μSv to 75 μSv, on ankle from 80 μSv to 110 μSv and on hand from 190 μSv to 240 μSv, respectively. For CAG+PTCA the observed levels were: on left eyebrow ridge from 170 μSv to 240 μSv, on right eyebrow ridge from 60 μSv to 160 μSv, on ankle from 180 μSv to 270 μSv and on hand from 410 μSv to 590 μSv, respectively. The wild range of doses for both eyebrow ridges was especially visible and depend on operation position with respect to X-ray tube. The eye-lens doses will be measured using a Randoman anthropomorphic phantom

  1. Stavitel železnic Eduard Bazika ve světle svých pamětí

    Czech Academy of Sciences Publication Activity Database

    Hlavačka, Milan

    2016-01-01

    Roč. 42, č. 1 (2016), s. 219-237 ISSN 0585-4172 Institutional support: RVO:67985963 Keywords : Bohemia * Eduard Bazika * Czech Tehnical High School Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  2. Simian virus 40 infection in humans and association with human diseases: results and hypotheses

    International Nuclear Information System (INIS)

    Barbanti-Brodano, Giuseppe; Sabbioni, Silvia; Martini, Fernanda; Negrini, Massimo; Corallini, Alfredo; Tognon, Mauro

    2004-01-01

    Simian virus 40 (SV40) is a monkey virus that was introduced in the human population by contaminated poliovaccines, produced in SV40-infected monkey cells, between 1955 and 1963. Epidemiological evidence now suggests that SV40 may be contagiously transmitted in humans by horizontal infection, independent of the earlier administration of SV40-contaminated poliovaccines. This evidence includes detection of SV40 DNA sequences in human tissues and of SV40 antibodies in human sera, as well as rescue of infectious SV40 from a human tumor. Detection of SV40 DNA sequences in blood and sperm and of SV40 virions in sewage points to the hematic, sexual, and orofecal routes as means of virus transmission in humans. The site of latent infection in humans is not known, but the presence of SV40 in urine suggests the kidney as a possible site of latency, as it occurs in the natural monkey host. SV40 in humans is associated with inflammatory kidney diseases and with specific tumor types: mesothelioma, lymphoma, brain, and bone. These human tumors correspond to the neoplasms that are induced by SV40 experimental inoculation in rodents and by generation of transgenic mice with the SV40 early region gene directed by its own early promoter-enhancer. The mechanisms of SV40 tumorigenesis in humans are related to the properties of the two viral oncoproteins, the large T antigen (Tag) and the small t antigen (tag). Tag acts mainly by blocking the functions of p53 and RB tumor suppressor proteins, as well as by inducing chromosomal aberrations in the host cell. These chromosome alterations may hit genes important in oncogenesis and generate genetic instability in tumor cells. The clastogenic activity of Tag, which fixes the chromosome damage in the infected cells, may explain the low viral load in SV40-positive human tumors and the observation that Tag is expressed only in a fraction of tumor cells. 'Hit and run' seems the most plausible mechanism to support this situation. The small tag

  3. Probióticos, Prebióticos y Salud: Evidencia Científica

    OpenAIRE

    Delgado, Susana; Salazar, Nuria; Sánchez García, Borja; Villar-Tajadura, M. Antonia; Nácher-Vázquez, Montserrat; Olivares, Marta

    2010-01-01

    24 páginas.-- Resúmenes de las ponencias presentadas al II Workshop Científico: Probióticos, prebióticos y salud: evidencia científica, celebrado en Madrid del 16 al 17 de Diciembre de 2010.-- et al.

  4. Women, feminism and social change in Argentina, Chile and Uruguay, 1980-1940

    Directory of Open Access Journals (Sweden)

    Guimoar Dueñas

    1996-01-01

    Full Text Available Asunción Lavrin. Women, feminism and social change in Argentina, Chile and Uruguay, 1890-1940. Lincoln and London: University of Nebraska Press, 1995, 481 páginas. Tercer volúmen de la serie Engendering Latin America.

  5. Prevención de accidentes de trabajo y enfermedades profesionales

    OpenAIRE

    Alonso Seco, José María

    2010-01-01

    En las páginas que siguen se ofrece una visión, necesariamente sintética porque cada actividad laboral presenta sus propias peculiaridades y grandes diferencias, sobre los aspectos comunes de la prevención de riesgos profesionales.

  6. Severe and unusual hepatic lesions asociated with alveolar echinococcosis (Echinococcus multilocularis) in a Gorilla g. gorilla

    OpenAIRE

    Polledo, Laura; Martínez-Fernández, B.; González, J.; Ferreras, Mª del Carmen; García Iglesias, M. J.; García Marín, Juan Francisco

    2011-01-01

    1 página.-- Trabajo presentado al II Iberic Meeting of Veterinary Pathology.--XVI Annual Meeting of the Portuguese Society of Animal Pathology.-- Annual Meeting of the Spanisch Society of Veterinary Anatomical Pathology. (Lisboa, Portugal, 1-3 de junio de 2011).

  7. Recurrent annual outbreaks of seasonal bovine congenital defects in north of Spain

    OpenAIRE

    Polledo, Laura; Martínez-Fernández, B.; González, J.; Pérez Martínez, C.; García Marín, Juan Francisco; García Iglesias, M. J.

    2011-01-01

    1 página.-- Trabajo presentado al II Iberic Meeting of Veterinary Pathology.--XVI Annual Meeting of the Portuguese Society of Animal Pathology.-- Annual Meeting of the Spanisch Society of Veterinary Anatomical Pathology. (Lisboa, Portugal, 1-3 de junio de 2011).

  8. A suspected case of calicivirus disease in an iberian hare (Lepus granatensis)

    OpenAIRE

    Polledo, Laura; Martínez-Fernández, B.; González, J.; Prieto, J. M.; Pérez Martínez, C.; García Iglesias, M. J.; García Marín, Juan Francisco

    2011-01-01

    1 página.-- Trabajo presentado al II Iberic Meeting of Veterinary Pathology.--XVI Annual Meeting of the Portuguese Society of Animal Pathology.-- Annual Meeting of the Spanisch Society of Veterinary Anatomical Pathology. (Lisboa, Portugal, 1-3 de junio de 2011).

  9. Avian tuberculosis in an eagle owl

    OpenAIRE

    Martínez-Fernández, B.; García Marín, Juan Francisco; Polledo, Laura; Pérez Pérez, Valentín; Delgado, L.; Ferreras, Mª del Carmen

    2011-01-01

    1 página.-- Trabajo presentado al II Iberic Meeting of Veterinary Pathology.--XVI Annual Meeting of the Portuguese Society of Animal Pathology.-- Annual Meeting of the Spanisch Society of Veterinary Anatomical Pathology. (Lisboa, Portugal, 1-3 de junio de 2011).

  10. Assessment of different monoclonal antibodies for the immunohistochemical detection of Mycobacterium avium subsp. paratuberculosis in tissue sections

    OpenAIRE

    Delgado, L.; Colstra, C.; Ferreras, Mª del Carmen; García Marín, Juan Francisco; Bakker, D.; Pérez Pérez, Valentín

    2011-01-01

    1 página.-- Trabajo presentado al II Iberic Meeting of Veterinary Pathology.--XVI Annual Meeting of the Portuguese Society of Animal Pathology.-- Annual Meeting of the Spanisch Society of Veterinary Anatomical Pathology. (Lisboa, Portugal, 1-3 de junio de 2011).

  11. National space, (trans)national cinema : Estonian film in the 1960s

    Index Scriptorium Estoniae

    Näripea, Eva, 1980-

    2011-01-01

    1960. aastatel kujunes ja kinnistus rahvusmaastiku kronotoop, mis mängis keskset rolli kogu hilisemas eesti filmis. Artiklis vaadeldakse lähemalt põlvkonnavahetuse n. ö. avalöögina käsitletava filmi "Ühe küla mehed" saamislugu

  12. Utilización de un compost de RSU (Villarrasa-Huelva) en tomate cultivado en invernadero

    OpenAIRE

    Madrid Díaz, Fernando; Trasierra, Manuel J.; López Núñez, Rafael; Murillo Carpio, José Manuel; Cabrera, Francisco

    1997-01-01

    7 páginas, 1 figura, 3 tabla y 13 referencias.-- Trabajo presentado en el VI Simposium Nacional - II Ibérico sobre nutrición mineral de las plantas, del 12-15 de noviembre 1996, en Sevilla, España.

  13. A multiproxy approach to evaluate biocidal treatm entson biodeteri orated majolica glazed tiles

    OpenAIRE

    Coutinho, M. L.; Miller, A. Z.; Jurado, Valme; Sáiz-Jiménez, Cesáreo; Macedo, M. F.

    2016-01-01

    23 páginas.-- 10 figuras.-- 6 tablas.-- 88 referencias.-- Supporting information Additional Supporting Information may be found in the online version of this article at the publisher’s web-site: http://dx.doi.org/10.1111/1462-2920.13380

  14. Self-Organized InGaAs Quantum Rings -- Fabrication and Spectroscopy

    OpenAIRE

    Lorke, Axel; García Martínez, Jorge Manuel; García Martínez, Jorge Manuel; Blossey, Ralf; Luyken, Richard Johannes; Petroff, Pierre M.

    2003-01-01

    13 páginas.-- The written version of the plenary and invited lectures of the Spring Meeting of the Condensed Matter Physics section of the Deutsche Physikalische Gesellschaft held from March 24 to 28, 2003 in Dresden, Germany.-- Kramer, Bernhard (Ed.).

  15. Identificación de polimorfismos en los genes FTO, MC4R y PCSK1 asociados a la obesidad en la población infantil y adulta mexicana /

    OpenAIRE

    Villalobos Comparan, Marisela

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Biomédicas, presenta Marisela Villalobos Comparan ; asesor Samuel Canizales Quinteros107 páginas : ilustracionesDoctorado en Ciencias Biomédicas UNAM, Facultad de Medicina, 2012

  16. Prevención de tabaquismo en mujeres adolescentes /

    OpenAIRE

    Calleja Bello, Nazira sustentante.

    2009-01-01

     tesis que para obtener el grado de Doctor en Psicología, presenta Nazira Calleja Bello ; asesor Susan Pick Steiner. 296 páginas : ilustraciones. Doctorado en Psicología UNAM, Facultad de Psicología, 2009

  17. Scorn misfortune. Subject, communication and the public in Peter Lemebel's narratives

    Directory of Open Access Journals (Sweden)

    Mirela Butnaru

    2012-11-01

    Full Text Available Fernando A. Blanco y Juan Poblete (Eds.. Desdén al infortunio. Sujeto, comunicación y público en la narrativa de Pedro Lemebel. Santiago de Chile: Editorial Cuarto Propio, 2010. 285 páginas.

  18. Schema.org, la millora de la visualització dels resultats en els cercadors i molt més

    Directory of Open Access Journals (Sweden)

    Sulé Duesa, Andreu

    2015-06-01

    Full Text Available Es descriuen les principals característiques de l'Schema.org, el vocabulari creat el 2011 per Google, Bing, Yahoo! i Yandex per marcar el contingut de les pàgines web i, d'aquesta manera, fer recognoscibles i comprensibles les seves dades als cercadors. Se n'exposa la principal aplicació avui dia, l'enriquiment de la informació que mostren els cercadors de cada pàgina web en els seus resultats, així com els fonaments de la seva estructura (entitats, propietats, valors, etc. i dels formats de codificació que accepta. També s'explica com marcar el contingut d'una pàgina web amb l'Schema.org, qui fa servir avui dia aquest vocabulari, i qui el manté i desenvolupa. Finalment, es valora breument la conveniència o no d'utilitzar l'Schema.org, així com les possibles aplicacions d'aquest vocabulari en la millora de les cerques i dels resultats dels cercadors (més precisió, més funcionalitats, etc., en el desenvolupament del web semàntic i, fins i tot, en el posicionament de les pàgines web en els cercadors (SEO.Se describen las principales características de Schema.org, el vocabulario creado en 2011 por Google, Bing, Yahoo! y Yandex para marcar el contenido de las páginas web y, de este modo, hacer reconocibles y comprensibles sus datos a los buscadores. Se expone su principal aplicación a día de hoy, el enriquecimiento de la información que muestran los buscadores de cada página web en sus resultados, así como los fundamentos de su estructura (entidades, propiedades, valores, etc. y de los formatos de codificación que acepta. También se explica cómo marcar el contenido de una página web con Schema.org, quién usa actualmente este vocabulario, y quién lo mantiene y desarrolla. Por último, se valora brevemente la conveniencia o no de utilizar Schema.org, así como las posibles aplicaciones de este vocabulario para mejorar las búsquedas y los resultados de los buscadores (más precisión, más funcionalidades, etc., en el

  19. Internal 40K radiation dose to Indians

    International Nuclear Information System (INIS)

    Ranganathan, S.; Someswara Rao, M.; Nagaratnam, A.; Mishra, U.C.

    2002-01-01

    A group of 350 Indians from both sexes (7-65 years) representing different regions of India was studied for internal 40 K radiation dose from the naturally occurring body 40 K, which was measured in the National Institute of Nutrition (NIN) whole-body counter. Although the 40 K radioactivity reached a peak value by 18 years in female (2,412 Bq) and by 20 years in male (3,058 Bq) and then varied inversely with age in both sexes, the radiation dose did not show such a trend. Boys and girls of 11 years had annual effective dose of nearly 185 mSv, which decreased during adolescence (165 mSv), increased to 175 mSv by 18-20 years in adults and decreased progressively on further ageing to 99 mSv in males and 69 mSv in females at 65 years. The observed annual effective dose (175 mSv) of the young adults was close to that of the ICRP Reference Man (176 mSv) and Indian Reference Man (175 mSv). With a mean specific activity of 55 Bq/kg for the subjects and a conversion coefficient close to 3 mSv per annum per Bq/kg, the average annual effective dose from the internal 40 K turned out to be 165 mSv for Indians. (author)

  20. The beneficial effects of l-cysteine on brain antioxidants of rats affected by sodium valproate.

    Science.gov (United States)

    Hamza, R Z; El-Shenawy, N S

    2017-11-01

    Oxidative stress caused by sodium valproate (SV) is known to play a key role in the pathogenesis of brain tissue. The present study was designed to evaluate the protective effect of l-cysteine (LC) on the antioxidants of brain tissue of rats. The animals were divided into six groups: control group 1 was treated with saline as vehicle, groups 2 and 3 were treated with low and high doses of SV (100 and 500 mg/kg, respectively), group 4 was treated with LC (100 mg/kg), and groups 5 and 6 were treated with low-dose SV + LC and high-dose SV + LC, respectively. All the groups were treated orally by gastric tube for 30 successive days. Some antioxidant parameters were determined. Brain tissue (cerebral cortex) of SV-treated animals showed an increase in lipid peroxidation (LPO) and reduction in activity of enzymatic antioxidant and total antioxidant levels. Histopathological examination of cerebral cortex of SV rats showed astrocytic swelling, inflammation, and necrosis. After 4 weeks of the combination treatment of SV and LC daily, results showed significant improvement in the activity of cathepsin marker enzymes and restored the structure of the brain. LC was able to ameliorate oxidative stress deficits observed in SV rats. LC decreased LPO level and was also able to restore the activity of antioxidant enzymes as well as structural deficits observed in the brain of SV animals. The protective effect of LC in SV-treated rats is mediated through attenuation of oxidative stress, suggesting a therapeutic role for LC in individuals treated with SV.

  1. Continuous stroke volume estimation from aortic pressure using zero dimensional cardiovascular model: proof of concept study from porcine experiments.

    Science.gov (United States)

    Kamoi, Shun; Pretty, Christopher; Docherty, Paul; Squire, Dougie; Revie, James; Chiew, Yeong Shiong; Desaive, Thomas; Shaw, Geoffrey M; Chase, J Geoffrey

    2014-01-01

    Accurate, continuous, left ventricular stroke volume (SV) measurements can convey large amounts of information about patient hemodynamic status and response to therapy. However, direct measurements are highly invasive in clinical practice, and current procedures for estimating SV require specialized devices and significant approximation. This study investigates the accuracy of a three element Windkessel model combined with an aortic pressure waveform to estimate SV. Aortic pressure is separated into two components capturing; 1) resistance and compliance, 2) characteristic impedance. This separation provides model-element relationships enabling SV to be estimated while requiring only one of the three element values to be known or estimated. Beat-to-beat SV estimation was performed using population-representative optimal values for each model element. This method was validated using measured SV data from porcine experiments (N = 3 female Pietrain pigs, 29-37 kg) in which both ventricular volume and aortic pressure waveforms were measured simultaneously. The median difference between measured SV from left ventricle (LV) output and estimated SV was 0.6 ml with a 90% range (5th-95th percentile) -12.4 ml-14.3 ml. During periods when changes in SV were induced, cross correlations in between estimated and measured SV were above R = 0.65 for all cases. The method presented demonstrates that the magnitude and trends of SV can be accurately estimated from pressure waveforms alone, without the need for identification of complex physiological metrics where strength of correlations may vary significantly from patient to patient.

  2. Replication of simian virus 40 in simian virus 40-transformed hamster kidney cells induced by mitomycin C or 60Co γ irradiation

    International Nuclear Information System (INIS)

    Rakusanova, T.; Smales, W.P.; Kaplan, J.C.; Black, P.H.

    1978-01-01

    Several clones of simian virus 40 (SV40)-transformed hamster kidney cells, which are heterogeneous for induction of infectious SV40, have been studied. SV40 yields are low after induction with 60 Co γ irradiation or mitomycin C. In order to clarify the mechanism(s) by which virus is produced in induced cells, we analyzed the replication of viral DNA and production of virion (V) antigen and infectious virus after induction in various clones as well as in lytically infected permissive cells. Cells replicating SV40 DNA or synthesizing V antigen were visualized by in situ hybridization and immunofluorescence techniques, respectively. Only some cells in induced cultures were found to produce SV40 and those which did were less efficient than lytically infected monkey cells. Mitomycin C or 60 Co γ irradiation acted by inducing more cells to replicate virus rather than by increasing the amount of SV40 released from individual cells. A greater proportion of cells could be induced to replicate SV40 DNA than to synthesize V antigen in all induced clones studied. Also, SV40 DNA replication was induced at lower doses of γ irradiation than the production of either V antigen or infectious virus suggesting that synthesis of late virus protein is more restricted in induced cells than is replication of SV40 DNA. These findings indicate that one of the effects of induction treatments on SV40-transformed hamster cells is an enhancement of the cells' capacity to support SV40 replication

  3. Video Quality Assessment Using Spatio-Velocity Contrast Sensitivity Function

    Science.gov (United States)

    Hirai, Keita; Tumurtogoo, Jambal; Kikuchi, Ayano; Tsumura, Norimichi; Nakaguchi, Toshiya; Miyake, Yoichi

    Due to the development and popularization of high-definition televisions, digital video cameras, Blu-ray discs, digital broadcasting, IP television and so on, it plays an important role to identify and quantify video quality degradations. In this paper, we propose SV-CIELAB which is an objective video quality assessment (VQA) method using a spatio-velocity contrast sensitivity function (SV-CSF). In SV-CIELAB, motion information in videos is effectively utilized for filtering unnecessary information in the spatial frequency domain. As the filter to apply videos, we used the SV-CSF. It is a modulation transfer function of the human visual system, and consists of the relationship among contrast sensitivities, spatial frequencies and velocities of perceived stimuli. In the filtering process, the SV-CSF cannot be directly applied in the spatial frequency domain because spatial coordinate information is required when using velocity information. For filtering by the SV-CSF, we obtain video frames separated in spatial frequency domain. By using velocity information, the separated frames with limited spatial frequencies are weighted by contrast sensitivities in the SV-CSF model. In SV-CIELAB, the criteria are obtained by calculating image differences between filtered original and distorted videos. For the validation of SV-CIELAB, subjective evaluation experiments were conducted. The subjective experimental results were compared with SV-CIELAB and the conventional VQA methods such as CIELAB color difference, Spatial-CIELAB, signal to noise ratio and so on. From the experimental results, it was shown that SV-CIELAB is a more efficient VQA method than the conventional methods.

  4. Resistance to cypermethrin and amitraz in Rhipicephalus (Boophilus microplus on the Santa Catarina Plateau, Brazil Situação da resistência do Rhipicephalus (Boophilus microplus à cipermetrina e amitraz no Planalto Catarinense, Brasil

    Directory of Open Access Journals (Sweden)

    Luana Paula Haubold Neis Veiga

    2012-06-01

    Full Text Available With the objective of evaluating Rhipicephalus (Boophilus microplus resistance to cipermetrina and amitraz, were collected engorged tick females from cattle on 20 farms on the Santa Catarina Plateau, in southern Brazil, between January of 2004 and May of 2006. Were also received 20 groups of engorged R. (B. microplus females, collected by cattle farmers requesting acaricidal efficacy (AE testing. Were performed in vitro tests, which consisted of immersing engorged females in cypermethrin (0.015% and in amitraz (0.025%. An AE >95% was considered indicative of effectiveness. Of the 20 collected groups, 18 (90% showed cypermethrin resistance and 1 (5% showed amitraz resistance. Of the 20 received groups, 19 (95% showed cypermethrin resistance and 2 (10% showed amitraz resistance. The AE of cypermethrin was found to be >95%, 85-94%, and 55-64%, respectively, in 4 (57.1%, 2 (28.6%, and 1 (14.3% of 7 reference groups, collected in the 1997-2001 period. The AE of amitraz was >95% in all of those groups. Among the groups of specimens received for analysis in that period, the AE of amitraz was >95% in 6 (85.71% and 75-84% in 1 (14.28%. R. (B. microplus resistance to acaricides is increasing on the Santa Catarina Plateau.Com os objetivos de avaliar a resistência do Rhipicephalus (Boophilus microplus à cipermetrina e ao amitraz no Planalto Catarinense, no período de janeiro de 2004 a maio de 2006, foram coletadas teleóginas em bovinos de 20 propriedades onde os produtores autorizaram a coleta (amostras por conveniência e recebidas teleóginas de mais 20 propriedades com objetivo de diagnóstico de eficácia. Os testes in vitro foram realizados por imersão de teleóginas em cipermetrina (0,015% e amitraz (0,025%. Considerando a eficácia igual ou superior a 95%, das 20 propriedades amostradas, 18 (90% apresentaram resistência à cipermetrina e uma (5% ao amitraz. Das 20 propriedades com teleóginas para diagnóstico, 19 (95% apresentaram resist

  5. Comparison between biventricular cine MRI and MR flow quantification in ascending aorta and pulmonary outflow tract for the assessment of intracardial shunt volumes

    International Nuclear Information System (INIS)

    Rominger, M.B.; Kluge, A.; Bachmann, G.F.; Dinkel, H.P.

    2002-01-01

    Purpose: Comparison between biventricular volumetric measurements and flow measurements in ascending aorta (Ao) and pulmonary outflow tract (Pu) for quantification of intracardial shunts, and evaluation of the combination of biventricular cine MRI with flow measurements for the assessment of RV and LV heart failure and valvular regurgitation (VR). Material and Methods: In 24 patients, right (RV) and left (LV) ventricular volumetric and flow measurements were performed in the Ao and Pu to assess the ratio of RV stroke volume (SV) or, respectively, pulmonary SV to LV SV or, respectively, systemic aortic SV (Qp/Qs). 34 patients without echocardiographically proven shunt or VR served as control group for measurement accuracy. Left-to-right shunt ratios were calculated from RV and LV SV, Pu and Ao SV, Pu and LV SV, and RV and Ao SV. Left ventricular VR was calculated by the difference of LV SV and Ao SV, and right ventricular VR by the difference of RV SV and Pu SV. Global systolic function was evaluated by biventricular cine MRI. Results: Intracardial shunts with Qp/Qs>1,16 can be quantified by flow measurements in Ao and Pu. Using biventricular volumetric measurements in cases without VR, requires a Qp/Qs>1.21. 17 of 18 intracardiac shunts were identified on MRI, and all 8 hemodynamically significant shunts were quantitatively confirmed. The diagnosis of complete shunt closure or absent shunt was correctly made in all 6 cases RV EF reduction was found in 6 of 24 patients. LV EF reduction was also found in 6 of 24 patients. Conclusion: Flow measurements in Ao and Pu are more accurate than biventricular cine MRI for the assessment of Qp/Qs ratios. Flow measurements in Ao and Pu combined with biventricular cine MRI enables the quantification of hemodynamic significant shunts, higher grade VR and biventricular global systolic function with a single examination. (orig.) [de

  6. Aquisition of age- and sex-dependent patient data for the calculation of annual radiation exposure in nuclear medicine: a German pilot study; Erfassung alters- und geschlechtsbezogener Daten nuklearmedizinischer Untersuchungen zur Berechnung der jaehrlichen Strahlenexposition in der BRD: Eine Pilotstudie

    Energy Technology Data Exchange (ETDEWEB)

    Schnell-Inderst, P.; Hacker, M.; Weiss, M.; Hahn, K. [Klinik und Poliklinik fuer Nuklearmedizin, Klinikum der Univ. Muenchen (Germany); Nosske, D.; Stamm-Meyer, A.; Brix, G. [Bundesamt fuer Strahlenschutz, Inst. fuer Strahlenhygiene, Neuherberg (Germany)

    2004-04-01

    Aim: a pilote study for estimation of radiation exposure due to diagnostic procedures in nuclear medicine using routine data of hospitals and practices in Germany. Methods: hospitals and practices willing to participate in the study supplied data of one year (1997), containing information on patients' identification number, age, sex, type of diagnostic procedure, radiopharmaceutical, administered activity, type of health insurance (private/public), inpatient/outpatient status, and so-called leistungsziffer, which describes the type of medical performances in Germany. The effective dose per examination was calculated according to ICRP 80. Mean, standard deviation, median, 5{sup th} and 95{sup th} percentiles of the effective dose were calculated, stratified by type of organ system and also by sex and age, including patients of {>=} 18 years. Results: 82 039 examinations from patients of 9 hospitals and practices were analyzed. The median (5-95{sup th} percentiles) of the effective dose per examination for all patients was 2.9 mSv (0.4-8.5 mSv); 1.2 examinations per patient and year were performed on average. The three most frequent examinations were bone scans (median 3.4 mSv; 2.9-5.1), thyroid (0.9 mSv; 0.4-2.2) and cardiovascular studies (7.3 mSv; 3.8-20.2). The median effective dose for 18 to 40 years old women was 1.0 mSv (0.4-5.8), for women between 41 and 65 years 2.2 mSv (0.4-7.3) and for women older than 65 years 2.4 mSv (0.5-7.6). The corresponding values for men were 2.6 mSv (0.3-7.6); 3.3 mSv (0.4-9.1), and 3.4 mSv (0.5-8.8). Conclusion: it was possible to gain an accurate determination of radiation exposure of diagnostic procedures in nuclear medicine by routine data. (orig.) [German] Ziel: Pilotstudie zur Ermittlung der Strahlenexposition in Deutschland bei Untersuchungen in der nuklearmedizinischen Diagnostik anhand von Routinedaten aus Kliniken und Praxen. Methoden: Kliniken und Praxen in Deutschland lieferten 1997 routinemaessig erhobene

  7. Mapping the outdoor gamma dose rate in Indonesia

    International Nuclear Information System (INIS)

    Iskandar, Dadong; Syarbaini, Sutarman; Bunawas, Kusdiana

    2008-01-01

    Full text: Indonesia is the largest archipelago in the world, comprising five main islands - Java, Sumatra, Sulawesi, Kalimantan and Papua - as well as 30 archipelagoes totaling 17,508 islands with about 6000 of those inhabited. Mapping the outdoor gamma dose rate in Indonesia is a research project conducted by National Nuclear Energy Agency since 2005 aiming to produce a baseline data map as an overview for planning purposes. In these three years 4 main islands has been measured. The grid system has been used in the research. In Sumatra Island the grid is 50 x 50 km 2 , while in Java 40 x 40 km 2 , in Kalimantan 60 x 60 km 2 , and in Sulawesi 40 x 40 km 2 . The gamma dose rates have been measured by Mini Gamma Ray Spectrometer Model GR-130 made by Exploranium-Canada. Figure 1 shows the map of outdoor gamma dose rate in Indonesia. Range of dose rate are in Sumatra from 22,96 ± 0,46 n Sv/h to 186,08 ± 3,72 n Sv/h, in Java 11,32 ± 0,72 n Sv/h to 127,54 ± 6,14 n Sv/h, in Kalimantan 10.72 ± 8.32 n Sv/h to 349,48 ± 57,21 n Sv/h, and in Sulawesi 17.7 ± 11,5 n Sv/h to 467 ± 102 n Sv/h. The arithmetic and geometric mean of dose rate in Indonesia are 68 n Sv/h and 53 n Sv/h, respectively. In general, outdoor gamma dose rate in Indonesia is in a normal range. There are some regions have anomaly of gamma dose rate, for examples at North Sumatra 186.08 ± 3,72 n Sv/h (N 2.12727, E 99.80909), at West Kalimantan 349,48 ± 57,21 n Sv/h (S 1.39507, E 110.57584), at West Sulawesi 487 ± 103 n Sv/h (S 2.95781, E 118.86995), etc. These data is very useful as a radiation baseline in Indonesia. (author)

  8. Predicting the Emergence of Sexual Violence in Adolescence.

    Science.gov (United States)

    Ybarra, Michele L; Thompson, Richard E

    2018-05-01

    This study aims to report the epidemiology of sexual violence (SV) perpetration for both female and male youth across a broad age spectrum. Additionally, the etiology of SV perpetration is examined by identifying prior exposures that predict a first SV perpetration. Six waves of data were collected nationally online, between 2006 and 2012, from 1586 youth between 10 and 21 years of age. Five types of SV were assessed: sexual harassment, sexual assault, coercive sex, attempted rape, and rape. To identify how prior exposures may predict the emergence of SV in adolescence, parsimonious lagged multivariable logistic regression models estimated the odds of first perpetrating each of the five types of SV within the context of other variables (e.g., rape attitudes). Average age at first perpetration was between 15 and 16 years of age, depending on SV type. Several characteristics were more commonly reported by perpetrators than non-perpetrators (e.g., alcohol use, other types of SV perpetration and victimization). After adjusting for potentially influential characteristics, prior exposure to parental spousal abuse and current exposure to violent pornography were each strongly associated with the emergence of SV perpetration-attempted rape being the exception for violent pornography. Current aggressive behavior was also significantly implicated in all types of first SV perpetration except rape. Previous victimization of sexual harassment and current victimization of psychological abuse in relationships were additionally predictive of one's first SV perpetration, albeit in various patterns. In this national longitudinal study of different types of SV perpetration among adolescent men and women, findings suggest several malleable factors that need to be targeted, especially scripts of inter-personal violence that are being modeled by abusive parents in youths' homes and also reinforced by violent pornography. The predictive value of victimization for a subsequent first SV

  9. In vivo capsular switch in Streptococcus pneumoniae--analysis by whole genome sequencing.

    Directory of Open Access Journals (Sweden)

    Fen Z Hu

    Full Text Available Two multidrug resistant strains of Streptococcus pneumoniae - SV35-T23 (capsular type 23F and SV36-T3 (capsular type 3 were recovered from the nasopharynx of two adult patients during an outbreak of pneumococcal disease in a New York hospital in 1996. Both strains belonged to the pandemic lineage PMEN1 but they differed strikingly in virulence when tested in the mouse model of IP infection: as few as 1000 CFU of SV36 killed all mice within 24 hours after inoculation while SV35-T23 was avirulent.Whole genome sequencing (WGS of the two isolates was performed (i to test if these two isolates belonging to the same clonal type and recovered from an identical epidemiological scenario only differed in their capsular genes? and (ii to test if the vast difference in virulence between the strains was mostly - or exclusively - due to the type III capsule. WGS demonstrated extensive differences between the two isolates including over 2500 single nucleotide polymorphisms in core genes and also differences in 36 genetic determinants: 25 of which were unique to SV35-T23 and 11 unique to strain SV36-T3. Nineteen of these differences were capsular genes and 9 bacteriocin genes.Using genetic transformation in the laboratory, the capsular region of SV35-T23 was replaced by the type 3 capsular genes from SV36-T3 to generate the recombinant SV35-T3* which was as virulent as the parental strain SV36-T3* in the murine model and the type 3 capsule was the major virulence factor in the chinchilla model as well. On the other hand, a careful comparison of strains SV36-T3 and the laboratory constructed SV35-T3* in the chinchilla model suggested that some additional determinants present in SV36 but not in the laboratory recombinant may also contribute to the progression of middle ear disease. The nature of this determinants remains to be identified.

  10. ASSOCIAÇÃO ENTRE A FRAÇÃO EXALADA DE ÓXIDO NÍTRICO E DADOS DA ESPIROMETRIA E O CONTROLE CLÍNICO DA ASMA EM CRIANÇAS E ADOLESCENTES

    Directory of Open Access Journals (Sweden)

    Luanda Dias da Silva Salviano

    2018-01-01

    Full Text Available RESUMO Objetivo: Avaliar a associação entre os valores da fração exalada de óxido nítrico (FeNO e do volume expiratório forçado no primeiro segundo (VEF1 e o grau de controle da asma, segundo proposta do Global Initiative for Asthma (GINA, em crianças e adolescentes asmáticos atendidos no Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira da Fundação Oswaldo Cruz(IFF/FIOCRUZ. Métodos: Estudo transversal, com revisão de prontuários de 90 asmáticos entre 7 e17 anos de idade, acompanhados no Ambulatório de Asma do IFF/FIOCRUZ e encaminhados ao setor de Prova de Função Respiratória (PFR entre março de 2013 e setembro de 2014. Após classificação segundo o GINA, os pacientes realizaram espirometria completa e medida da FeNO. Posteriormente, foram separados em dois grupos: em uso regular e não regular de corticosteroide inalatório (CI, independentemente do padrão ventilatório na espirometria. Resultados: Observou-se associação entre os valores de VEF1 e o grau de controle da asma segundo o GINA (p=0,001 em todos os pacientes analisados, independentemente do uso de CI, mas não houve associação entre os valores de VEF1 e os níveis da FeNO. Conclusões: A correlação observada entre o GINA e o VEF1 reforça a importância da espirometria no seguimento clínico desses pacientes. Embora não tenha sido detectada associação entre o valor da FeNO e o grau de controle da asma e o VEF1,a FeNO pode constituir um método precoce para detectar inflamação nas vias aéreas, antes mesmo dos sintomas e das alterações espirométricas.

  11. Modeling energy price dynamics: GARCH versus stochastic volatility

    International Nuclear Information System (INIS)

    Chan, Joshua C.C.; Grant, Angelia L.

    2016-01-01

    We compare a number of GARCH and stochastic volatility (SV) models using nine series of oil, petroleum product and natural gas prices in a formal Bayesian model comparison exercise. The competing models include the standard models of GARCH(1,1) and SV with an AR(1) log-volatility process, as well as more flexible models with jumps, volatility in mean, leverage effects, and t distributed and moving average innovations. We find that: (1) SV models generally compare favorably to their GARCH counterparts; (2) the jump component and t distributed innovations substantially improve the performance of the standard GARCH, but are unimportant for the SV model; (3) the volatility feedback channel seems to be superfluous; (4) the moving average component markedly improves the fit of both GARCH and SV models; and (5) the leverage effect is important for modeling crude oil prices—West Texas Intermediate and Brent—but not for other energy prices. Overall, the SV model with moving average innovations is the best model for all nine series. - Highlights: • We compare a variety of GARCH and SV models for fitting nine series of energy prices. • We find that SV models generally compare favorably to their GARCH counterparts. • The SV model with moving average innovations is the best model for all nine series.

  12. A ciência e a tecnologia n’A Capital: da página de fait-divers à página de ciência

    OpenAIRE

    Fonseca, Rui Brito

    2009-01-01

    Durante muito tempo, a ciência e a tecnologia foram olhadas, pela população em geral, como mais um tema de entretenimento e curiosidade, a par de outros. Para a imprensa portuguesa, mormente a imprensa de cariz “popular”, a ciência e a tecnologia também foram durante algum tempo olhadas como um assunto de entretenimento e curiosidade. Partindo da análise, quantitativa e qualitativa, de cerca de 2000 artigos sobre ciência e tecnologia, publicados no jornal diário, pago e de c...

  13. Evaluación de la actividad del caseinato de sodio (casna) sobre la inducción de la mielopoyesis y linfopoyesis /

    OpenAIRE

    Domínguez Melendez, Vanihamin

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Biológicas, presenta Vanihamin Dominguez Melendez ; asesor Edelmiro Santiago Osorio99 páginas : ilustracionesDoctorado en Ciencias Biológicas UNAM, Facultad de Estudios Superiores Zaragoza, 2012

  14. Evaluación de la conducta impulsiva y desarrollo de autocontrol en adolescentes testigos de violencia familiar /

    OpenAIRE

    Negrete Cortés, Agustin Jaime

    2012-01-01

     tesis que para obtener el grado de Doctorado en Psicología, presenta Agustin Jaime Negrete Cortes ; asesor Ariel Vite Sierra98 páginas : ilustracionesDoctorado en Psicología UNAM, Facultad de Psicología, 2012

  15. Efecto del flujo turbulento, la condición superficial, la presencia de ácido acético y un compuesto orgánico usado como inhibidor de corrosión sobre la cinética electroquímica del acero api 5l x52 en soluciones con co2 disuelto /

    OpenAIRE

    Olvera Martínez, María Elena

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ingeniería, presenta Maria Elena Olvera Martinez ; asesor Juan Mendoza Flores310 páginas : ilustracionesDoctorado en Ingeniería UNAM, Facultad de Química, 2012

  16. Diferencias en la asociación de complejos respiratorios de yarrowia lipolytica en respuesta a los requerimientos energéticos /

    OpenAIRE

    Guerrero Castillo, Sergio

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Bioquímicas, presenta Sergio Guerrero Castillo ; asesor Salvador Uribe Carvajal135 páginas : ilustracionesDoctorado en Ciencias Bioquímicas UNAM, Facultad de Química, 2012

  17. Sobre la historia de la traducción en España

    Directory of Open Access Journals (Sweden)

    Manuel Bruña Cuevas

    2011-01-01

    Full Text Available A propósito de la obra de Francisco Lafarga y Luis Pegenaute (eds., Diccionario histórico de la traducción en España (Madrid, Gredos, 2009; 1232 páginas. ISBN: 978-84-249-3626-6.

  18. Recall bias did not affect perceived magnitude of change in health-related functional status

    NARCIS (Netherlands)

    Middel, B; Goudriaan, H; De Greef, M; Stewart, R; van Sonderen, E; Bouma, J; de Jongste, M

    Background and Objective: it was hypothesized that within an invasively treated group and within a group that improved in gina pectoris no difference in effect size would occur between prospective and retrospective measures. Furthermore, it was hypothesized that assessment of perceived change at

  19. Implementation of a General Reform Plan in the Army of the Dominican Republic (IMPLEMENTACI N DE UNA REFORMA INTEGRAL EN EL EJ RCITO DE LA REPUBLICA DOMINICANA)

    Science.gov (United States)

    2013-06-13

    CIENCIAS Y ARTES MILITARES Estudios Generales Por TTE.COR. MANUEL A. CARRASCO GUERRERO B.A., Technical University of Santiago-Utesa......otras sinceramente muchas gracias. v TABLA DE CONTENIDO Páginas MAESTRÍA EN CIENCIAS Y ARTES MILITARES PAGINA DE APROBACION DE LA TESIS

  20. Arcillas y biocarbones para la eliminación de contaminantes de origen agrícola en aguas: ensayo en filtro a escala precomercial

    OpenAIRE

    Durán, Esperanza

    2017-01-01

    297 páginas.-- Tesis para obtener el título de Doctor por la Universidad de Sevilla con la calificación de Sobresaliente Cum laude.-- Tutora: Dra. Isabel González Díez, Profesora Titular de la Universidad de Sevilla

  1. Comunicación Pública y Gestión Estratégica Municipal. Un estudio exploratorio sobre la agenda temática, libro de Concepción Campillo Alhama

    Directory of Open Access Journals (Sweden)

    Kathy Matilla

    2013-05-01

    Full Text Available Comunicación Pública y Gestión Estratégica Municipal. Unestudio exploratorio sobre la agenda temática. Concepción Campillo Alhama, 2011, España, Instituto Andaluz de Administración Pública, 411 páginas.

  2. Distribution of Radiation Exposure from Natural Radiation in Big Cities

    International Nuclear Information System (INIS)

    Udiyani, P.M.; Ahmad, Yus R.

    2000-01-01

    The measurement of radiation exposure from the natural radiation in the big city in Java such as Jakarta, Bandung, Semarang, Yogyakarta, and Surabaya have be done. Based on radiation dose and population at the sample location, the dose collective and risk probability will be know. The maximal exposure at Yogyakarta is 0.291 mSv/year and the minimal exposure at Surabaya is 0.216 mSv/year. Collective dose at Jakarta is 1.649.526 men mSv/year; Bandung 124.844 men mSv/year; Semarang : 64.558 men mSv/year; Yogyakarta 136.188 men mSv/year; and Surabaya 145.152 men mSv/year. The person probability of radiation disease at jakarta is 16.49 person/year, Bandung is 1.24 person/year, Semarang 1.64 person/year, Yogyakarta is 1.36 person/year, and Surabaya is 1.45 person/year

  3. Radiation dose from cigarette tobacco

    International Nuclear Information System (INIS)

    Papastefanou, Constantin

    2008-01-01

    The radioactivity in tobacco leaves collected from 15 different regions of Greece before cigarette production was studied in order to estimate the effective dose from cigarette tobacco due to the naturally occurring primordial radionuclides, such as 226 Ra and 210 Pb of the uranium series and 228 Ra of the thorium series and or man-made produced radionuclides, such as 137 Cs of Chernobyl origin. Gamma-ray spectrometry was applied using Ge planar and coaxial type detectors of high resolution and high efficiency. It was concluded that the annual effective dose due to inhalation for adults (smokers) for 226 Ra varied from 42.5 to 178.6 μSv y -1 (average 79.7 μSv y -1 ), while for 228 Ra from 19.3 to 116.0 μSv y -1 (average 67.1 μSv y -1 ) and for 210 Pb from 47.0 to 134.9 μSv y -1 (average 104.7 μSv y -1 ), that is the same order of magnitude for each radionuclide. The sum of the effective dose of the three natural radionuclides varied from 151.9 to 401.3 μSv y -1 (average 251.5 μSv y -1 ). The annual effective dose from 137 Cs of Chernobyl origin was three orders of magnitude lower as it varied from 70.4 to 410.4 μSv y -1 (average 199.3 μSv y -1 ). (author)

  4. Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation

    International Nuclear Information System (INIS)

    Tateishi, Yoshihisa; Sasabe, Eri; Ueta, Eisaku; Yamamoto, Tetsuya

    2008-01-01

    Reactive oxygen species (ROS) have important roles in various physiological processes. Recently, several novel homologues of the phagocytic NADPH oxidase have been discovered and this protein family is now designated as the Nox family. We investigated the involvement of Nox family proteins in ionizing irradiation-induced ROS generation and impairment in immortalized salivary gland acinar cells (NS-SV-AC), which are radiosensitive, and immortalized ductal cells (NS-SV-DC), which are radioresistant. Nox1-mRNA was upregulated by γ-ray irradiation in NS-SV-AC, and the ROS level in NS-SV-AC was increased to approximately threefold of the control level after 10 Gy irradiation. The increase of ROS level in NS-SV-AC was suppressed by Nox1-siRNA-transfection. In parallel with the suppression of ROS generation and Nox1-mRNA expression by Nox1-siRNA, ionizing irradiation-induced apoptosis was strongly decreased in Nox1-siRNA-transfected NS-SV-AC. There were no large differences in total SOD or catalase activities between NS-SV-AC and NS-SV-DC although the post-irradiation ROS level in NS-SV-AC was higher than that in NS-SV-DC. In conclusion, these results indicate that Nox1 plays a crucial role in irradiation-induced ROS generation and ROS-associated impairment of salivary gland cells and that Nox1 gene may be targeted for preservation of the salivary gland function from radiation-induced impairment

  5. Investigation of Contributions of Cosmic Radiation to Background Dose with Altitude at Ahmadu Bello University, Zaria, Nigeria

    International Nuclear Information System (INIS)

    Uwaechia, F.C.; Zakari, Y.I.; Ibeanu, I.G.E.

    2014-01-01

    This study investigated the contributions of cosmic radiation to background dose at Ahmadu Bello University, Zaria, Nigeria using two portable survey meters (Rados -120 and FH 40F2 ). The work was conducted in two phases (dry and rainy seasons).The recorded gamma dose rates were observed to proportionally increase with increase in altitude, that is from ground floor (altitude 3.52 m above sea level) to the eight floor (altitude 30.08 m above sea level). In a similar manner, there were observed variations in the seasonal results-the dry season data were consistently higher than the rainy season data. The mean measured indoor and outdoor gamma dose rates for the two seasons (rainy and dry seasons) were 210.0nSv/h and 279.4nSv/h, and 231.3nSv/h and 368.8nSv/h at the ground floor and the eight floor, respectively. The rainy season result repeated itself on a particular very heavy rainy day with a mean measured gamma dose rate of 204.4nSv/h and 267.4nSv/h at the ground floor and eight floor, respectively. The annual indoor and outdoor mean effective dose for a 1000hour working period was calculated as 0.32 μSv and 0.35 μSv at the ground floor and 0.68 μSv and 0.76 μSv the 8 th floor, respectively (for inside and outside the building).

  6. Portales de destinos turísticos en Internet: una reflexión estratégica

    Directory of Open Access Journals (Sweden)

    Miralbell, Oriol

    2001-03-01

    Full Text Available La denominación de portal en el ámbito de Internet es de uso relativamente reciente y fue iniciada primero por los norteamericanos, siendo su significado en inglés y en español casi el mismo. Aunque originalmente el portal ha pretendido ser la página de entrada a Internet, la que un usuario usa para entrar a navegar por Internet, con la actual proliferación de portales, podríamos decir que esta definición se usa para referirse a una página web que hace las funciones de pórtico de entrada a un espacio informativo temático (a veces de gran alcance, es decir, de un conglomerado de múltiples temas, con lo que se pretende homologar el mundo virtual de Internet con los espacios sociales y culturales de la vida real.

  7. MISIÓN SIN CONQUISTA. ACOMPAÑAMIENTO DE COMUNIDADES INDÍGENAS AUTÓCTONAS COMO PRÁCTICA MISIONERA ALTERNATIVA (RESEÑA

    Directory of Open Access Journals (Sweden)

    Hilario Wynarzcyk

    2012-06-01

    Full Text Available El libro de 338 páginas es una colección de artículos organizada por misioneros menonitas. Willis Horst es estadounidense mientras que Ute Mueller-Eckhardt y su marido Frank Paul son alemanes. Todos ellos con sus familias se retiraron ya de la misión entre los años 2008 y 2009. Este libro coronan una extensa experiencia en las provincias del Chaco y Formosa acompañando pastoralmente a las comunidades de indígenas tobas (así conocidos históricamente por el público argentino y en la actualidad comúnmente denominados “Pueblo Qom”. Horts, Willis; Mueller-Eckhardt,Ute; Paul Frank.2011. Misión Sin Conquista.Acompañamiento de comunidades indígenas autóctonas como práctica misionera alternativa.Editorial Kairos, Buenos Aires, Argentina, 338 páginas.

  8. Facebook como herramienta de marketing/comunicación para la atracción de estudiantes internacionales: un análisis de Chile y España

    Directory of Open Access Journals (Sweden)

    José Luis Meza Orellana

    2015-11-01

    Full Text Available Este trabajo analiza el uso de Facebook como herramienta de marketing internacional en universidades de Chile y España. Se estudió el 93% de las universidades de ambos países mediante el método de análisis de contenido. Los resultados muestran que el uso de Páginas de Facebook corresponde, en su mayoría, a universidades con mayor matrícula internacional. Hay una relación directa entre la cantidad de estudiantes extranjeros y el uso de Fanpage como canal de comunicación de marketing. Por el contrario, los mejores desempeños corresponden a universidades con menos extranjeros. Existe una relación inversa entre el desempeño en esas páginas y la cantidad de estudiantes extranjeros, pero directa, entre el número de estudiantes y la presencia en Facebook mediante Fan Page.

  9. Relation of size of seminal vesicles on ultrasound to premature ejaculation

    Directory of Open Access Journals (Sweden)

    Zhi-Wei Hong

    2017-01-01

    Full Text Available Myriad biological factors have been proposed to explain premature ejaculation (PE. However, data correlating PE with seminal vesicles (SVs are sparse. The study aimed to evaluate the relationship between the size of SV and PE. The cross-sectional study included 44 outpatients with PE and 44 volunteers without PE, and the size of SV was compared. Self-estimated intravaginal ejaculatory latency time, the Premature Ejaculation Diagnostic Tool (PEDT, the International Index of Erectile Function-15, and the National Institutes of Health-Chronic Prostatitis Symptom Index were used for assessment of symptoms. Compared to the control group, the PE group had significantly higher mean anterior-posterior diameter (APD of SV (P < 0.001. The optimal mean APD of SV cutoff level was 9.25 mm for PE. In the PE group, PEDT was also higher with a mean APD of SV ≥9.25 mm compared with mean APD of SV <9.25 mm. PEDT was significantly correlated with the mean APD of SV (r = 0.326, P = 0.031. The seminal plasma proteins were compared between six PE and six matched control cases by mass spectrometry and it was shown that 102 proteins were at least 1.5-fold up- or down-regulated. Among them, GGT1, LAMC1, and APP were significantly higher in the PE group. These results indicated that men with a larger mean APD of SV might have a higher PEDT score. Transrectal ultrasound of SV should be considered in the evaluation of patients with premature ejaculation. SV might be a potential target for the treatment of patients with PE and ultrasound change in SV.

  10. Precollege Sexual Violence Perpetration and Associated Risk and Protective Factors Among Male College Freshmen in Georgia.

    Science.gov (United States)

    Salazar, Laura F; Swartout, Kevin M; Swahn, Monica H; Bellis, Alexandra L; Carney, Jhetari; Vagi, Kevin J; Lokey, Colby

    2018-03-01

    Sexual violence (SV) perpetration on college campuses is a serious and prevalent public health issue in the U.S. In response, incoming male freshmen are mandated to receive SV prevention programming. To provide a more effective response, however, we need to understand the SV behaviors of male freshmen before they arrive on campus and the associated factors that contribute to risk and that afford protection, areas that have received limited attention. Male freshmen (N = 1,133) across 30 selected 4-year colleges and universities throughout the state of Georgia were recruited for a longitudinal study on SV perpetration. Levels of precollege SV as well as a range of covariates were assessed at baseline. Self-reported SV perpetrators were compared with nonperpetrators on demographic and hypothesized covariates deemed either risk or protective; then risk and protective models were analyzed using binary logistic regression. Weighted analyses revealed that 19.3% self-reported perpetrating SV before college. Before starting college, young men who reported more sexual media consumption, heavy episodic drinking, hypermasculine beliefs, and peers who endorsed SV were more likely to have a history of SV perpetration at college matriculation. Alternatively, men with more knowledge of effective sexual consent and stronger family functioning were less likely to arrive to college with an SV perpetration history. A significant proportion of incoming male freshmen have perpetrated SV previously. Colleges and universities need to assess incoming freshmen for risk behaviors and negative beliefs and to offer both primary and secondary preventions to more effectively reduce further perpetration. Copyright © 2017 The Society for Adolescent Health and Medicine. All rights reserved.

  11. Staff radiation doses during eight years in a nuclear medicine radiopharmacy

    International Nuclear Information System (INIS)

    Jansen, S.E.; Aswegen, A. van; Loetter, M.G.; Herbst, C.P.; Otto, A.C.; Orange Free State Univ., Bloemfontein

    1994-01-01

    Staff working in the radiopharmacy and nursing staff responsible for injecting radionuclides are being monitored constantly in our department. We report here on the effective doses and doses to the hands received by staff at two hospitals during 8 years from January 1985 to December 1992. In addition to the doses determined monthly by the South African Bureau of Standards' Radiation Protection Service (SABS), radiation doses received to the hands and whole body were measured every week using lithium fluoride thermoluminescent dosimetry (TLD). The highest total radiation dose received in any one year by any one person at hospital A was 233.53 mSv to the hands, and 10.20 mSv and 8.37 mSv to the whole body depending on the dosemeter used. The corresponding values for hospital B were 54.05 mSv to the hands and 6.94 mSv and 4.43 mSv to the whole body. If only one radiographer should do all the work calculated highest dose received would be 447.06 mSv to the hands and 9.68 mSv SABS effective dose. The radiation doses to staff were well within the limits prescribed. (Author)

  12. Estimation of outdoor and indoor effective dose and excess lifetime cancer risk from Gamma dose rates in Gonabad, Iran

    Energy Technology Data Exchange (ETDEWEB)

    Jafaria, R.; Zarghania, H.; Mohammadia, A., E-mail: rvzreza@gmail.com [Paramedical faculty, Birjand University of Medical Sciences, Birjand (Iran, Islamic Republic of)

    2017-07-01

    Background gamma irradiation in the indoor and outdoor environments is a major concern in the world. The study area was Gonabad city. Three stations and buildings for background radiation measurement of outdoor and indoor were randomly selected and the Geiger-Muller detector (X5C plus) was used. All dose rates on display of survey meter were recorded and mean of all data in each station and buildings was computed and taken as measured dose rate of that particular station. The average dose rates of background radiation were 84.2 nSv/h for outdoor and 108.6 nSv/h for indoor, maximum and minimum dose rates were 88.9 nSv/h and 77.7 nSv/h for outdoor measurements and 125.4 nSv/h and 94.1 nSv/h for indoor measurements, respectively. Results show that the annual effective dose is 0.64 mSv, which compare to global level of the annual effective dose 0.48 mSv is high. Estimated excess lifetime cancer risk was 2.24×10{sup -3} , indicated that it is large compared to the world average value of 0.25×10{sup -3}. (author)

  13. La política monetaria de objetivos de inflación en México /

    OpenAIRE

    Rodríguez Benavides, Domingo

    2011-01-01

     tesis que para obtener el grado de Doctor en Economía, presenta Domingo Rodríguez Benavides ; asesor Ignacio Perrotini Hernández. 121 páginas : ilustraciones. Doctorado en Economía UNAM, Facultad de Economía, 2011

  14. UOC Restaurant : gestió integral d'un restaurant

    OpenAIRE

    Vidal Andrade, Marta

    2012-01-01

    Desenvolupament d'una aplicació amb Microsoft.net per la gestió d'un restaurant i de la seva plana web. Desarrollo de una aplicación con Microsoft.net para la gestión de un restaurante y de su página web.

  15. Evaluación agronómica de residuos-orgánicos mediante caracterización físico-química y bioensayos de laboratorios e invernadero

    OpenAIRE

    García de Vinuesa Fernández, Daniel

    1999-01-01

    105 páginas.-- 8 figuras.-- 8 tablas.-- 94 referencias.-- El presente trabajo ha sido realizado en el marco del Proyecto de Investigación: SUSTRATOS ORGÁNICOS EN CULTIVOS DE FRESÓN Y NARANJO: VALORACIÓN AGRONÓMICA y MEDIOAMBIENTAL (Proyecto AGF96-0914)

  16. Metodología para la evaluación del nivel de sustentabilidad de materiales de construcción. Caso de estudio : el acero /

    OpenAIRE

    Hernández Ayón, Francisco Javier sustentante.

    2009-01-01

     tesis que para obtener el grado de Doctor en Arquitectura, presenta Francisco Javier Hernández Ayón ; asesor David Morillón Galvéz. ix, 141 páginas : ilustraciones. Doctorado en Arquitectura UNAM, Facultad de Arquitectura, 2009

  17. Impacto ambiental del turismo de buceo en los arrecifes coralinos de Cozumel, México /

    OpenAIRE

    Santander Botello, Luis Carlos sustentante.

    2009-01-01

     tesis que para obtener el grado de Doctor en Geografía, presenta Luis Carlos Santander Botello ; asesor Enrique Propin Frejomil. 303 páginas : ilustraciones. Doctorado en Geografía UNAM, Facultad de Filosofía y Letras, 2009

  18. Estudio mediante teoría de funcionales de la densidad de las propiedades electrónicas, estructurales y energéticas de complejos organometálicos de cúmulos de hierro con benceno en fase gaseosa : fen(c6h&)m, n<=7, m>=4 /

    OpenAIRE

    Valencia Quiroz, Israel

    2012-01-01

     tesis que para obtener el grado de Doctorado en Ciencias Químicas, presenta Israel Valencia Quiroz ; asesor Miguel Castro Martínez149 páginas : ilustracionesDoctorado en Ciencias Químicas UNAM, Facultad de Química, 2012

  19. Diseño y evaluación de un programa de habilidades para la salud en niños en comunidades rurales /

    OpenAIRE

    Anaya González, Cecilia Irene sustentante.

    2009-01-01

     tesis que para obtener el grado de Doctor en Psicología, presenta Cecilia Irene Anaya González ; asesor Susan Pick Steiner. 197 páginas : ilustraciones. Doctorado en Psicología UNAM, Facultad de Psicología, 2009

  20. Racismo en Chile. La piel como marca de la inmigración (María Emilia Tijoux)

    OpenAIRE

    Gonzalo Díaz Letelier

    2016-01-01

    Racismo en Chile. La piel como marca de la inmigración Editora: María Emilia TIJOUX — Editorial: Universitaria. Santiago, Chile. Año: 2016 Páginas: 284 ISBN: 978-956-11-2502-5 Autor de la reseña: Gonzalo DÍAZ LETELIER

  1. Světový trh kakaa

    OpenAIRE

    Voglová, Karolína

    2014-01-01

    This bachelor thesis deals with the global cocoa market. The aim of this thesis is to analyse the global cocoa market and indentify the future prospects of this market. The first chapter deals with the general charakteristics of this commodity, i.e. the history of cocoa growing, types of cocoa, cocoa cultivation and cocoa producing areas, and it is processed by using the methods of synthesis and comparison. Another chapter is the analysis of the global cocoa market, which presents the greates...

  2. Så faldt Damokles-sværdet!

    DEFF Research Database (Denmark)

    Fledelius, Karsten

    2013-01-01

    En i udlandet stærkt kritiseret russisk lov om registrering af NGOer, herunder selvangivelse som "udenlandske agent" hvis man modtager støtte fra udlandet, er nu sat i fuld gang, trods kritik bl.a. af den tyske kansler Angela Merkel...

  3. Sexual Violence Perpetration by Adolescents in Dating versus Sam-Sex Peer Relationships: Differences in Associated Risk and Protective Factors

    Directory of Open Access Journals (Sweden)

    Kathleen C Basile

    2013-08-01

    Full Text Available Introduction: Little is known about the risk and protective factors for youth sexual violence (SV perpetration across different types of relationships. This study examined factors associated with perpetrating SV against a dating partner and a same-sex peer.Methods: Analyses were based on data from a survey conducted in 2004 with public school boys and girls in grades 7, 9, 11, and 12 (N=4,131 in a high-risk, urban school district in the United States. SV perpetration was defined broadly to include forcing someone, about the same age and of the same or opposite sex as the respondent, to have sex or to do something sexual that they did not want to do. Analyses examined the associations between risk and protective factors and SV perpetration, adjusting for SV victimization and demographic characteristics.Results: Findings revealed that 2.1% of respondents reported perpetration against a same-sex peer and 3.2% reported perpetration against a date during the past 12 months. Victims of SV for each relationship type were more likely than non-victims to perpetrate SV. A combination of factors across the individual, relationship, and community level were significantly associated with SV perpetration and there were both shared and unique factors across the relationship types.Conclusion: Data suggest that programs to prevent SV perpetration for both relationship types should start when students are young, with particular focus on middle school boys. Prevention efforts should have slightly different foci to address these 2 types of SV perpetration. [West J Emerg Med. 2013;14(4:329–340.

  4. Extremity and eye lens dosimetry for medical staff performing vertebroplasty and kyphoplasty procedures

    International Nuclear Information System (INIS)

    Struelens, L; Schoonjans, W; Vanhavere, F; Schils, F; De Smedt, K

    2013-01-01

    Measurements of doses to hands, legs and eyes are reported for operators in four different hospitals performing vertebroplasty or kyphoplasty. The results confirm that occupational doses can be high for interventional spine procedures. Extremity and eye lens doses were measured with thermoluminescent dosimeters positioned on the ring fingers, wrists, legs and near the eyes of interventional radiologists and neurosurgeons, over a period of 15 months. Doses were generally larger on the left side for all positions monitored. The median dose to the left finger was 225 μSv per procedure, although a maximum of 7.3 mSv was found. The median dose to the right finger was 118 μSv, but with an even higher maximum of 7.7 mSv. A median left eye dose of 34 μSv (maximum 836 μSv) was found, while the legs received the lowest doses with a median of 13 μSv (maximum 332 μSv) to the left leg. Annual dose to the hand assessed by the cumulated doses almost reached the annual dose limit of 500 mSv, while annual dose to the eyes exceeded the eye lens dose limit of 20 mSv yr −1 . Different x-ray systems and radiation protection measures were tested, like the use of lead gloves and glasses, tweezers, cement delivery systems and a magnetic navigation system. These measurements showed that doses can be significantly reduced. The use of lead glasses is strongly recommended for protection of the eyes. (paper)

  5. Sexual Violence Perpetration by Adolescents in Dating versus Same-Sex Peer Relationships: Differences in Associated Risk and Protective Factors

    Science.gov (United States)

    Hamburger, Merle E.; Swahn, Monica H.; Choi, Colleen

    2013-01-01

    Introduction: Little is known about the risk and protective factors for youth sexual violence (SV) perpetration across different types of relationships. This study examined factors associated with perpetrating SV against a dating partner and a same-sex peer. Methods: Analyses were based on data from a survey conducted in 2004 with public school boys and girls in grades 7, 9, 11, and 12 (N = 4,131) in a high-risk, urban school district in the United States. SV perpetration was defined broadly to include forcing someone, about the same age and of the same or opposite sex as the respondent, to have sex or to do something sexual that they did not want to do. Analyses examined the associations between risk and protective factors and SV perpetration, adjusting for SV victimization and demographic characteristics. Results: Findings revealed that 2.1% of respondents reported perpetration against a same-sex peer and 3.2% reported perpetration against a date during the past 12 months. Victims of SV for each relationship type were more likely than non-victims to perpetrate SV. A combination of factors across the individual, relationship, and community level were significantly associated with SV perpetration and there were both shared and unique factors across the relationship types. Conclusion: Data suggest that programs to prevent SV perpetration for both relationship types should start when students are young, with particular focus on middle school boys. Prevention efforts should have slightly different foci to address these 2 types of SV perpetration. PMID:23930146

  6. Individual radiation doses. Annual report 1995

    International Nuclear Information System (INIS)

    Bergman, L.

    1995-05-01

    During the year we measured whole body doses on 10226 bearers, distributed as follows: 0-0,5 mSv on 8816 persons, 0,6-1,0 mSv on 693 persons, 1,1-5,0 on 678 persons, >5 mSv on 39 persons. At higher dose than 4 mSv/4 weeks, the reason to the irradiation will be investigated. 2 figs, 2 tabs

  7. Prevalence of Sexual Violence and its Association with Depression among Male and Female Patients with Risky Drug Use in Urban Federally Qualified Health Centers.

    Science.gov (United States)

    Bone, Curtis W; Goodfellow, Amelia M; Vahidi, Mani; Gelberg, Lillian

    2018-02-01

    Sexual violence (SV) is common; however, the prevalence of SV and its long term sequela vary geographically and among subpopulations within the USA. As such, the aims of this study are the following: (1) to determine the prevalence of SV, (2) to identify correlates of SV, and (3) to determine if SV is associated with depression among male and female risky drug users in urban Federally Qualified Health Centers (FQHCs) in Los Angeles. This study includes adult patients of five urban FQHCs who self-reported risky drug use. We identified survivors of SV and those experiencing depression through survey questions that queried, before or after age 18, "Were you ever sexually assaulted, molested or raped?" and with the RAND Mental Health Index (MHI-5). We utilized Pearson's chi-square tests to assess predictors of SV and logistic regression to assess for an association between SV and depression. Data collection took place from February 2011 to November 2012. Of the 334 study patients, 49% of females and 25% of males reported surviving SV. Exposure to SV, (both before 18 years of age and after 18 years of age) was the strongest predictor of depression among men and women in this study (OR 4.7, p < 0.05). These data demonstrate that sexual violence is prevalent in this urban FQHC population and is strongly associated with depression. Providers should consider screening both men and women with risky drug use for SV while health systems should continue to align mental health and primary care services to appropriately care for these extremely vulnerable patients. Trial Registration Clinical Trials. gov ID NCT01942876, Protocol ID DESPR DA022445, http://www.clinicaltrials.gov.

  8. Measurement and evaluation of personal radiation dose during 18F-FDG PET imaging

    International Nuclear Information System (INIS)

    Lu Ning; Wang Jing; Qiao Hongqing; Deng Jinglan; Li Guoquan; Zhou Yi

    2004-01-01

    Objective: To measure and evaluate the personal radiation dose for medical staff and patient accompanying persons in PET imaging, in order to offer the reference data for clinical radiation protection. Methods: Analysis of γ-ray radiation dose rate was performed on 30 medical staff members by using radiation dose meter during each medical procedure in injection room and scanning room , and the instantaneous, 1 and 2 h dose rate at 0.1, 0.5, 1.0 and 2.0 m from the mid-thorax of the patient received injection of the isotope were also measured. Then the mean dose per medical procedure per person and the assuming annual dose at different working sites were all calculated. Results: The mean personal doses per procedure were: left hand (30.0 ± 8.0) μSv, right hand (6.0 ± 1.5) μSv, whole-body (0.5 ± 0.1) μSv for syringe preparation; hand (3.00 ± 0.75) μSv, whole-body (1.27 ± 0.20) μSv for injection; (9.9 ± 1.4) μSv for imaging operation; (310 ± 91) μSv for close contact accompanying persons. Annual dose for staff members working in different sites were: left hand (16.63 ± 4.41) mSv, right hand (6.45 ± 1.23) mSv, whole-body (1.18 ± 0.15) mSv in the injection room; whole-body (4.99 ± 0.70) mSv in the imaging room. Conclusion: Under the normal operational conditions, the dose received by staff members and accompanying persons do not exceed the annual limit for professional and non-professional persons that has published as GuoBiao safe standard (GBSS)

  9. Dose reduction in multi-slice CT of the heart by use of ECG-controlled tube current modulation (''ECG pulsing''): phantom measurements

    International Nuclear Information System (INIS)

    Poll, L.W.; Cohnen, M.; Brachten, S.; Moedder, U.; Ewen, K.

    2002-01-01

    To evaluate the effect of ECG-controlled tube current modulation on radiation exposure in retrospectively-ECG-gated multislice CT (MSCT) of the heart. Material and methods: Three different cardiac MSCT protocols with different slice collimation (4 x 1, and 4 x 2.5 mm), and a pitch-factor of 1.5 and 1.8 were investigated at a multi-slice CT scanner Somatom Volume Zoom, Siemens. An anthropomorphic Alderson-Rando phantom was equipped with LiF-Thermoluminescence dosimeters at several organ sites, and effective doses were calculated using ICRP-weighting factors. Scan protocols were performed with ECG-controlled tube current modulation ('ECG pulsing') at two different heart rates (60 and 80 bpm). These data were compared to previous data from MSCT of the heart without use of 'ECG pulsing'. Results: Radiation exposure with (60 bpm) and without tube current modulation using a 2.5 mm collimation was 1.8 mSv and 2.9 mSv for females, and 1.5 mSv and 2.4 mSv for males, respectively. For protocols using a 1 mm collimation with a pitch-factor of 1.5 (1.8), radiation exposure with and without tube current modulation was 5.6 (6.3) mSv and 9.5 (11.2) mSv for females, and 4.6 (5.2) mSv and 7.7 (9.2) mSv for males, respectively. At higher heart rates (80 bpm) radiation exposure is increased from 1.5-1.8 mSv to 1.8-2.1 mSv, using the 2.5 mm collimation, and from 4.6-5.6 mSv to 5.9-7.2 mSv, for protocols using 1 mm collimation. Conclusions: The ECG-controlled tube current modulation allows a dose reduction of 37% to 44% when retrospectively ECG-gated MSCT of the heart is performed. The tube current - as a function over time - and therefore the radiation exposure is dependent on the heart rate. (orig.) [de

  10. Sexual Violence and Intimate Partner Violence in College Women with a Mental Health and/or Behavior Disability.

    Science.gov (United States)

    Bonomi, Amy; Nichols, Emily; Kammes, Rebecca; Green, Troye

    2018-03-01

    We address questions about (1) how college women with a disability experience sexual violence (SV) and intimate partner violence (IPV) across partners, including disability-specific abuse and (2) how SV/IPV impacts psychological, behavioral, physical, and academic life domains. Twenty-seven female college students (mean age, 21.2; 66.6% white; 66.6% heterosexual) were randomly sampled from university registrar records. To be eligible for the study, students had to have at least one experience of SV/IPV since age 18 and a disability (88.8% reported one or more mental health conditions; 11.1% reported other conditions, such as attention deficit and hyperactivity disorder; with the majority of women indicating their disability preceded SV/IPV victimization). Using the Centers for Disease Control and Prevention's definitions of SV/IPV as guides, clinically trained master's level interviewers conducted semistructured interviews to ascertain SV/IPV patterns across students' three most recent relationships and related life impacts. SV/IPV was pervasive in college women with a disability, within hookup settings and/or recurring SV/IPV with a long-term partner. For some women, SV spanned multiple abusive partners. For women in relationships marked by chronic abuse, in addition to SV, the relationship dynamic included disability-specific abuse, social isolation, threats/intimidation, and technology-related abuse. For women experiencing SV events within hookup settings, alcohol was a common facilitator, with some abusers using a disability to manipulate a sexual connection. All but one participant reported exacerbated adverse mental health consequences (e.g., depression, anxiety, post-traumatic stress disorder, suicidal ideation/attempts, stress) after victimization. These adverse mental health consequences coincided with adverse behavioral (e.g., becoming less social, avoiding usual study lounge areas on campus), physical (e.g., problems sleeping, bruising, pregnancy

  11. A review of radiation risk estimates

    International Nuclear Information System (INIS)

    1991-06-01

    Three authoritative reports (UNSCEAR-1988, BEIR-V-1990, and ICRP-1990 Recommendations) on risk estimates have been reviewed and compared to previous risk estimates published by the same organizations. The ICRP now uses the term 'probability' in place of the term 'risk'. For fatal cancers, the new ICRP probability estimates are 5.0 x 10 -2 Sv -1 for a population of all ages and 4.0 x 10 -2 Sv -1 for a population of working age. For serious hereditary effects summarized over all generations, the ICRP probability coefficients are 1.0 x 10 -2 Sv -1 for a population of all ages and 0.6 x 10 -2 Sv -1 for a population of working age. For prenatal irradiation, at 8 - 15 weeks after conception, there may be a decrease of 30 I.Q. points per Sv and a risk of cancer which may lie in the range of 2 to 10 x 10 -2 Sv -1 . Based mainly on the new probability estimates the ICRP recommends a limit on effective dose of 20 mSv per year, averaged over 5 years (100 mSv in 5 years) with the further provision that the effective dose should not exceed 50 mSv in any single year. For public exposure the ICRP recommends an annual limit on effective dose of 1 mSv. However, in special circumstances, a higher value of effective dose could be allowed in a single year provided that the average over 5 five years does not exceed 1 mSv per year. Once pregnancy has been declared, the conceptus should be protected by applying a supplementary equivalent dose limit to the surface of the woman's abdomen of 2 mSv for the remainder of the pregnancy and by limiting intakes of radionuclides to about 1/20 of the annual limit on intake. A brief survey of epidemiological studies of workers and the risks from radon and thoron progeny is also included. (110 refs, 29 tabs., 10 figs.)

  12. Role of microRNA in Aggressive Prostate Cancer

    Science.gov (United States)

    2015-09-01

    Interleukin enhancer-binding factor 2 OS=Homo sapiens GN=ILF2 PE=1 SV=2 ACACA_HUMAN Acetyl-CoA carboxylase 1 OS=Homo sapiens GN=ACACA PE=1 SV=2...I3L1L3_HUMAN Myb-binding protein 1A (Fragment) OS=Homo sapiens GN=MYBBP1A PE=4 SV=1 XRCC5_HUMAN X-ray repair cross-complementing protein 5 OS=Homo sapiens GN...XRCC5 PE=1 SV=3 SFPQ_HUMAN Splicing factor, proline- and glutamine-rich OS=Homo sapiens GN=SFPQ PE=1 SV=2 ATPA_HUMAN ATP synthase subunit alpha

  13. Dose assessment of radiation workers at atomic energy authority (Inchas site) 1964-1994. Vol. 4

    Energy Technology Data Exchange (ETDEWEB)

    Gommaa, M A; Youssed, S K [Nuclear Research Center, Atomic Energy, Cairo (Egypt)

    1996-03-01

    The present study reports the 1994 radiation exposure of workers at the nuclear research center, of the at atomic energy authority of egypt. Results indicated that 50% of the workers receive annual exposure of 3 mSv, 15% of workers received annual exposure less than 1 mSv, 28% of the workers received annual exposure of 7.5 mSv, and 7% of the workers received annual exposure in the range 10 to 20 mSv. The average annual exposure was estimated as 4.8 mSv. This result is comparable with the 1988-1993 results for nuclear research center workers. 3 tabs.

  14. Does iterative reconstruction lower CT radiation dose: evaluation of 15,000 examinations.

    Directory of Open Access Journals (Sweden)

    Peter B Noël

    Full Text Available PURPOSE: Evaluation of 15,000 computed tomography (CT examinations to investigate if iterative reconstruction (IR reduces sustainably radiation exposure. METHOD AND MATERIALS: Information from 15,000 CT examinations was collected, including all aspects of the exams such as scan parameter, patient information, and reconstruction instructions. The examinations were acquired between January 2010 and December 2012, while after 15 months a first generation IR algorithm was installed. To collect the necessary information from PACS, RIS, MPPS and structured reports a Dose Monitoring System was developed. To harvest all possible information an optical character recognition system was integrated, for example to collect information from the screenshot CT-dose report. The tool transfers all data to a database for further processing such as the calculation of effective dose and organ doses. To evaluate if IR provides a sustainable dose reduction, the effective dose values were statistically analyzed with respect to protocol type, diagnostic indication, and patient population. RESULTS: IR has the potential to reduce radiation dose significantly. Before clinical introduction of IR the average effective dose was 10.1±7.8mSv and with IR 8.9±7.1mSv (p*=0.01. Especially in CTA, with the possibility to use kV reduction protocols, such as in aortic CTAs (before IR: average14.2±7.8mSv; median11.4mSv /with IR:average9.9±7.4mSv; median7.4mSv, or pulmonary CTAs (before IR: average9.7±6.2mSV; median7.7mSv /with IR: average6.4±4.7mSv; median4.8mSv the dose reduction effect is significant(p*=0.01. On the contrary for unenhanced low-dose scans of the cranial (for example sinuses the reduction is not significant (before IR:average6.6±5.8mSv; median3.9mSv/with IR:average6.0±3.1mSV; median3.2mSv. CONCLUSION: The dose aspect remains a priority in CT research. Iterative reconstruction algorithms reduce sustainably and significantly radiation dose in the clinical routine

  15. Absorbed Dose and Effective Dose for Lung Cancer Image Guided Radiation Therapy(IGRT) using CBCT and 4D-CBCT

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Dae Yong; Lee, Woo Suk; Koo, Ki Lae; Kim, Joo Seob; Lee, Sang Hyeon [Dept. of Radiation Oncology, GangNeung Asan Hospital, Gangneung (Korea, Republic of)

    2016-06-15

    To evaluate the results of absorbed and effective doses using CBCT and 4D-CBCT settings for lung cancer. This experimental study. Measurements were performed using a Anderson rando phantom with OSLD(optically stimulated luminescent dosimeters). It was performed computed tomography(Lightspeed GE, USA) in order to express the major organs of the human body. Measurements were obtained a mean value is repeated three times each. Evaluations of effective dose and absorbed dose were performed the CL-IX-Thorax mode and Truebeam-Thorax mode CBCT. Additionally, compared Truebeam-Thorax mode CBCT with Truebeam-Thorax mode 4D-CBCT(Four-dimensional Cone Beam Computed Tomography). Average absorbed dose in the CBCT of CL-IX was measured in lung 2.505cGy, heart 2.595cGy, liver 2.145cGy, stomach 1.934cGy, skin 2.233cGy, in case of Truebeam, It was measured lung 1.725cGy, heart 2.034cGy, liver 1.616cGy, stomach 1.470cGy, skin 1.445cGy. In case of 4D-CBCT, It was measured lung 3.849cGy, heart 4.578cGy, liver 3.497cGy, stomach 3.179cGy, skin 3.319cGy Average effective dose, considered tissue weighting and radiation weighting, in the CBCT of CL-IX was measured lung 2.164mSv, heart 2.241mSVv, liver 0.136mSv, stomach 1.668mSv, skin 0.009mSv, in case of Turebeam, it was measured lung 1.725mSv, heart 1.757mSv, liver 0.102mSv, stomach 1.270mSv, skin 0.005mSv, In case of 4D-CBCT, It was measured lung 3.326mSv, heart 3.952mSv, liver 0.223mSv, stomach 2.747mSv, skin 0.013mSv. As a result, absorbed dose and effective Dose in the CL-IX than Truebeam was higher about 1.3 times and in the 4D-CBCT Truebeam than CBCT of Truebeam was higher about 2.2times However, a large movement of the patient and respiratory gated radiotherapy may be more accurate treatment in 4D-CBCT. Therefore, it will be appropriate to selectively used.

  16. A theoretical and experimental dose rate study at a multipurpose gamma irradiation facility in Ghana

    International Nuclear Information System (INIS)

    Sackey, Tracey A.

    2015-01-01

    Radiation dose rate monitoring out at the Radiation Technology Centre (RTC) of the Ghana Atomic Energy Commission (GAEC) to establish the safety or otherwise of staff at the occupied areas is presented. The facility operates a rectangular source of Co-60 gamma with an having activity of 27.4kCi as at March 2015 and has 14 workers. The aim of the research was determine by means of practical and theoretical evaluations shielding effectiveness of the irradiation chamber. This was to ensure that occupationally exposed workers are not over exposed or their exposures do not exceed the regulatory limits of 7.5μSv/h or 50mSv per annum. The study included dose rate measurements at controlled areas, evaluation of personnel dose history, comparison of experimental and theoretical values and determination of whether the shielding can support a. 18.5PBq (500kCi) Co-60 source. Practical dose rate measurements when the source was in the irradiation position was carried out using a Thermo Scientific Rad-Eye Gamma Survey Meter in the controlled areas of the facility which included the control room, electric room, deionizer room, on top of the roof of irradiation chamber (specifically above the roof plugs) and the two entrances to the irradiation chamber; the personnel door and the goods door. Background reading was found to be 0.08±0.01μSv/h whilst the average dose rates at the two entrances to the irradiation chamber (i e.,- the personnel door and the goods door) were measured to be 0.090μSv/h and 0.109μSv/h respectively. Practical measurements at the roof plugs produced average values of 0.135μSv/h. A particular point on the roof marked as plug-3 produced a relatively higher dose rate of 8.151μSv/h due probably to leakage along the cable to the drive motor. Measurements in the control room, electrical room and deionizer room had average readings of 0.116μSv/h, 0.089μSv/h and 0.614μSv/h respectively. All these average values were below the regulatory limits of 7.5μSv

  17. Natural radionuclides in the human body; Natuerliche Radionuklide im menschlichen Koerper

    Energy Technology Data Exchange (ETDEWEB)

    Voelkle, Hansruedi [Fribourg Univ. (Switzerland). Physikdept.

    2017-08-01

    Natural radionuclides in the human body produce worldwide a medium annual radiation exposure of 0.31 mSv. 0.17 mSv are due to potassium-40 (K-40) per year, 0.12 mSv per year are due to radionuclides from the uranium and thorium decay series, less than 0.02 mSv due to cosmogenic radionuclides. Natural radioactivity is therefore the largest exposure source, anthropogenic exposure is comparatively marginal.

  18. Programación de gráficos 3D con Mathematica, DrawGraphics, CurvesGraphics, LiveGraphics3D y JavaView

    OpenAIRE

    Mora Flores, Walter; Instituto Tecnológico de Costa Rica; Figueroa, Geovanni; Instituto Tecnológico de Costa Rica

    2015-01-01

    Se muestra como integrar las herramientas: Mathematica (y los paquetes DrawGraphics y CurvesGraphics), LiveGraphics3D, JavaView y html, para crear algunas figuras 3D las cuales se pueden incrustar en páginas Web independientes y con posibilidad de interacción.

  19. Ganancia obtenida mediante recogida selectiva de semilla en un huerto semillero de Pinus pinaster Ait.

    OpenAIRE

    Merlo, E.; Zas Arregui, Rafael; Fernández López, Josefa

    2005-01-01

    8 páginas, 3 figuras, 3 tablas -- Comunicación presentada al 4º Congreso Forestal Español que se celebró en Zaragoza entre el 26 y el 30 de septiembre de 2005 bajo el lema ‘La ciencia forestal: Respuestas para la sostenibilidad’.

  20. Desarrollo de una interfaz gráfica mediante Galaxy para Qiime

    OpenAIRE

    Navas Molina, Jose Antonio

    2012-01-01

    En aquest projecte s'ha desenvolupat un paquet Python que permet la integració del pipeline QIIME a la plataforma Galaxy de forma automàtica. També s'ha replicat la pàgina web FastUniFrac utilitzant scripts de QIIME integrats en Galaxy.