Paulo D. Ferreira Júnior
Full Text Available This work presents the first data on incubation temperature of Phrynops geoffroanus (Schweigger, 1812 in a natural environment, and provides information on nest predation, hatching success and size of offspring born in the nests on sandy beaches along the Upper Xingu River. Thirty-one P. geoffroanus nests were found, of which eleven were completely predated, mainly by Cerdocyon thous (Linnaeus, 1766. Incubation was completed in nine out of the 17 nests protected by netting. The nests presented an average of 13.1 eggs and were distributed over the various geomorphological sectors of the nine sampled beaches. The size and weight of the hatchlings varied significantly between nests, and the incubation period in protected nests lasted for 76.5 days, less than reported for controlled incubation in the laboratory. Daily variation in incubation temperature in the three nests monitored for temperature was lower in those situated in fine sand sediments. Incubation temperature varied from 22 to 39 C and may have affected hatching success, which reached 60.8% in protected nests. Nest distribution in different geomorphological sectors indicated the plasticity of P. geoffroanus in terms of variation in nesting environment, which partly explains the species' broad geographical distribution.
Bruno O. Ferronato
Full Text Available During 2006 and 2007, we collected free-ranging Phrynops geoffroanus, from two anthropogenically altered rivers in southeastern Brazil. Oral microbiological samples were taken for isolation of aerobic and facultative anaerobic bacteria; a physical examination was performed;and we evaluated possible effects on the turtles’ health. Twenty-nine species of bacteria were isolated in Piracicaba River turtles (n=10, and twenty-four species in Piracicamirim stream turtles (n=8, most of them gram-negative. In both sites, potential pathogens for reptiles were: Escherichia coli, Klebsiella pneumoniae, Enterobacter agglomerans, Citrobacter freundii, and Bacillus sp. Although boatpropeller lesions were common on the carapace of the turtles, we have not found turtles with signs of clinical diseases. The oral bacterial microbiota of P. geoffroanus inhabiting the Piracicaba River basin are composed of a diverse microbe spectrum, and long-term studies of the effects of pollution and traumatic injuries on this population and its microbial flora are warranted.
Piña, C I; Lance, V A; Ferronato, B O; Guardia, I; Marques, T S; Verdade, L M
The Piracicaba River basin is considered the most disturbed river basin in the state of São Paulo. Considerable amounts of agricultural residues are seasonally drained into the river, and the region is also highly urbanized and industrialized with an incipient sewage treatment system. The presence of heavy metals has been previously reported for the water and riverbed in Piracicaba river basin. In this study we evaluated 13 heavy metals in the blood of 37 Geoffroy's side-necked turtles, Phrynops geoffroanus, from Piracicaba River and Piracicamirim Creek, one of its tributaries. Blood levels of As, Co, Cr, Se and Pb varied among sites, whereas Sn varied between males and females. However, no obvious pathology was detected. Serum level of Cu (2,194 ng g(-1)) and Pb (1,150 ng g(-1)) found in this study are the highest ever described for any reptile; however, no clinical symptoms have been detected in the present study. There is no information about the time scale of such contamination, which could be currently subclinical and yet lead to a breakdown in the population reproductive success in a few years. Based on the present study, legal enforcement is urged in order to locate and extirpate heavy metal sources in the Piracicaba River basin. In addition, monitoring should include humans and commercial fish consumed in local markets.
Geoffroy's side-necked turtle [Phrynops geoffroanus (Schweigger, 1812), Testudines: Chelidae] as a model for evolutionary ecotoxicology: relationship between environmental contamination, conditions and genetic variability.
Venancio, L P R; Zuccari, D A P C; Bonini-Domingos, C R
of five genetic groups that reflected changes in habitat created by damming and siltation, which initiate separation processes (barriers) between sub-populations. The relationship between the data obtained for biochemical parameters, condition factors and genetic diversity was analyzed by heterozygosity-fitness correlation. The negative relationship observed may be explained by the profile of structural and ecological changes in the populations studied, indicating the important influence of humans on the biology of natural populations. Therefore, Phrynops geoffroanus shows adaptation to environmental contamination, and ecological changes and possible loss of habitat are altering the genetic diversity of the populations studied. This is the first study evaluating all these aspects of P. geoffroanus simultaneously in natural populations in Brazil, using this species as a model.
Full Text Available The Neotropical turtle Phrynops williamsi (Testudines, Chelidae is a scarcely studied species distributed in southern Brazil, north-eastern Argentina, Paraguay and Uruguay. We report herein the finding of a juvenile specimen, it measured 40 mm of carapace length and was collected in February 2013 by Tiago Delpino at San Gregorio de Polanco (32°36'S, 55°50'W, Tacuarembó Department, in a streamlet tributary of the Negro River (MNHN 9487. This new record suggests that P. williamsi is widely distributed over the Negro River basin, at the southern boundaries of the species geographic range.
Franco L. Souza
Full Text Available A review on activity patterns, reproduction, and feeding habits in Brazilian chelid turtles (Testudines, Chelidae.The knowledge on natural history of some Brazilian chelonian species is still scarce what makes it difficult to ascertain ecological andevolutionary histories. Data like this, however, can be used as fundamental tools for species management and conservation. A survey was made on the activity patterns, reproduction, and feeding behavior of Brazilian Chelidae species (freshwater sidenecked turtles available in the literature. A clear lack of information regarding natural history is evident for most species. The species diversity and the biological importance of this taxon regard it as an interesting target to be studied. Since several Chelidae behaviors (including those reported in this study are considered stereotyped, I suggest that laboratory studies should be encouraged as a way of complementing the field research.
William P. McCord
Full Text Available Relationships among turtle species loosely categorized within the South American genus Phrynops are explored. Three once recognized genera (Batrachemys, Mesoclemmys and Phrynops that were demoted to subgenera, and then synonymized with Phrynops, are demonstrated to warrant full recognition based on morphometric analysis, skull osteology, and mitochondrial and nuclear gene sequencing. Mesoclemmys is resurrected from the synonymy of Phrynops as a monotypic genus including M. gibba. The genus Rhinemys, previously a synonym of Phrynops, is resurrected for the species R. rufipes. Ranacephala gen. nov. is described to inciude the species R hogei. The genus Batrachemys is resurrected from the synonymy of Phrynops and inciudes B. dahli, B. nasuta B. raniceps, B. tuberculata, and B. zuliae. The taxon vanderhaegei is placed in Bufocephala gen. nov. The genus Phrynops is redefined to include the taxa P. geoffroanus, P. hilarii, P. tuberosus and P.williamsi. Ciadistic analysis of morphological data supports this taxonomy. A new species of Batrachemys is described from the western Amazon region, and is distinguished by having facial markings in juveniles, a relatively wide head, and a flattened shell. The new species, B. heliostemma sp. nov., is sympatric with and most similar to the recently resurrected form Batrachemys raniceps in the upper Amazonian region of Peru and adjacent Brazil, Ecuador, Venezuela, and Colombia. Lastly, morphometric data from living and museum specimens of all species of Batrachemys are presented.Se investigaron las afinidades entre las especies de tortugas agrupadas casualmente dentro del género suramericano Phrynops. Tres géneros anteriormente reconocidos (Batrachemys, Mesoclemmys, y Phrynops, que fueron subsecuentemente colocados como sub-géneros, y luego puestos bajo sinonimia de Phrynops, se reconocen ahora como géneros válidos basándose en análisis morfométrico, osteología craneal y de secuencias génicas, nucleares y
Anestesia de cágado-de-barbicha Phrynops geoffroanus Schweigger, 1812 (Testudines com a associação midazolan e propofol = Anaesthesia of geoffroy’s side-necked turtle Phrynops geoffroanus Schweigger, 1812 (Testudines with the association of midazolam and propofol
André Luiz Quagliatto Santos
Full Text Available Os cágados apresentam fisiologia e morfologia únicas, que se diferenciam em muitos aspectos dos mamíferos. Por isso, a monitoração do paciente durante um processo anestésico ou sedativo deve ser realizada, porque dosagens e drogas com resultados benéficos em mamíferos têm-se mostrado inadequados para estas espécies. Foram utilizados dez exemplares de Phrynops geoffroanus, provenientes do rio Uberabinha, no município de Uberlândia, Estado de Minas Gerais (licença RAN/IBAMA nº 035/2006, os quais foram anestesiados com o protocolo midazolan 2 mg kg-1 IM-1 e propofol 10 mg kg-1 IV-1. Osbatimentos cardíacos dos exemplares foram monitorados com o aparelho Doppler Vascular Eletrônico nos tempos 0’, 10’, 30’, 60’, 120’ e 180’ pós-anestésico e, durante o período transanestésico, os cágados foram observados em relação aos parâmetros estipulados (locomoção, relaxamento muscular, manipulação, estímulos dolorosos nos membrostorácicos e pélvicos. O propofol (10 mg kg-1 IV-1 se mostrou um anestésico de rápida indução, com duração média da anestesia ideal de 66’. O midazolan na dose de 2 mg kg-1 IM-1 foi um pré-anestésico eficiente, promovendo relaxamento muscular e facilidade de manipulação do animal. A associação de anestésicos utilizada obteve bons resultados, promovendo analgesia por um tempo médio de 97’5’’. Não houve significante diminuição da frequência cardíaca e não foi observada apneia nos quelônios anestesiados.Turtles present a unique morphology and physiology and differ in many ways from mammalians. Therefore, anesthetic monitoring of the patient during sedation and anesthesia should be known, because the drugs and the dosages used successfully in mammals may prove to be inadequate in these species. Ten Phrynops geoffroanus were used, from the Uberabinha River, in Uberlândia, Minas Gerais State (license RAN/IBAMA no. 035/2006 which were anesthetized with midazolam (2 mg kg-1
Anestesia de cágado-de-barbicha Phrynops geoffroanus Schweigger, 1812 (Testudines com a associação midazolan e propofol - DOI: 10.4025/actascibiolsci.v31i3.674 Anaesthesia of geoffroy’s side-necked turtle Phrynops geoffroanus Schweigger, 1812 (Testudines with the association of midazolam and propofol - DOI: 10.4025/actascibiolsci.v31i3.674
Andréa Cristina Scarpa Bosso
Full Text Available Os cágados apresentam fisiologia e morfologia únicas, que se diferenciam em muitos aspectos dos mamíferos. Por isso, a monitoração do paciente durante um processo anestésico ou sedativo deve ser realizada, porque dosagens e drogas com resultados benéficos em mamíferos têm-se mostrado inadequados para estas espécies. Foram utilizados dez exemplares de Phrynops geoffroanus, provenientes do rio Uberabinha, no município de Uberlândia, Estado de Minas Gerais (licença RAN/IBAMA nº 035/2006, os quais foram anestesiados com o protocolo midazolan 2 mg kg-1 IM-1 e propofol 10 mg kg-1 IV-1. Os batimentos cardíacos dos exemplares foram monitorados com o aparelho Doppler Vascular Eletrônico nos tempos 0’, 10’, 30’, 60’, 120’ e 180’ pós-anestésico e, durante o período transanestésico, os cágados foram observados em relação aos parâmetros estipulados (locomoção, relaxamento muscular, manipulação, estímulos dolorosos nos membros torácicos e pélvicos. O propofol (10 mg kg-1 IV-1 se mostrou um anestésico de rápida indução, com duração média da anestesia ideal de 66’. O midazolan na dose de 2 mg kg-1 IM-1 foi um pré-anestésico eficiente, promovendo relaxamento muscular e facilidade de manipulação do animal. A associação de anestésicos utilizada obteve bons resultados, promovendo analgesia por um tempo médio de 97’5’’. Não houve significante diminuição da frequência cardíaca e não foi observada apneia nos quelônios anestesiados.Turtles present a unique morphology and physiology and differ in many ways from mammalians. Therefore, anesthetic monitoring of the patient during sedation and anesthesia should be known, because the drugs and the dosages used successfully in mammals may prove to be inadequate in these species. Ten Phrynops geoffroanus were used, from the Uberabinha River, in Uberlândia, Minas Gerais State (license RAN/IBAMA no. 035/2006 which were anesthetized with midazolam (2 mg kg
João F.M. Rodrigues
Full Text Available Here, we described a noninvasive method for sexing freshwater turtles by stimulating penile eversion. We immobilized the neck and limbs of animals using fingers and, after some seconds, turtles everted their penis. This method was tested in 33 male Phrynops tuberosus, and 28 everted the penis. The efficiency of the method was not dependent of animal size, which reinforces its applicability. Our method allows sexing turtles in the field, avoiding killing the animal or causing major injuries in order to assess the sex.
Full Text Available A type of locomotor behavior observed in animals with rigid bodies, that can be found in many animals with exoskeletons, shells, or other forms of body armor, to change direction, is the turning behavior. Aquatic floated-turning behavior among rigid bodies animals have been studied in whirligig beetles, boxfish, and more recently in freshwater turtle, Chrysemys picta. In the laboratory we observed a different kind of turning movement that consists in an underwater turning movement during foraging, wherein the animal pivoted its body, using one of the hindlimbs as the fixed-point support in the substratum. We describe, analyze and quantify this movement during foraging in Hydromedusa maximiliani, using observations made in the laboratory. We studied 3 adult specimens (2 males, 1 female and 2 non-sexed juveniles of H. maximiliani. They were kept individually in an aquarium filled with water and small fish. They were filmed, in dorsal view, at 30 frames per second. Sequences were analyzed frame by frame and points were marked on limbs and shell to enable analysis of variation in limb flexion and extension, as well as rotation movements. While foraging, turtles frequently turned their bodies, using one hind leg as the pivot point. This underwater turning movement, in addition to slow movements with the neck stretched, or staying nearly immobile and scanning the surroundings with lateral movements of the neck (in arcs up to 180°, and fast attacks of neck, may increase prey capture rates.
Cabrera Mario R.
Full Text Available The plastral spotting variation in the chelid turtle Phrynops hilarii (Duméril & Bibron, 1835 in relation to sex, size, and geographic procedence of individuals was analyzed. States for qualitative characters were analyzed using non-parametric tests. Quantitative characters (shell and scute measurements were standardized for body size by linear regression against carapace length, and were subjected to principal components analysis and canonical discriminant function analysis. Results suggest that increased plastral spotting is a polymorphic ontogenetic trait in P. hilarii. Neither hatchlings nor juveniles have plastral pattern moderately or heavily pigmented. The simplest pattern, however, may persist without changes in some adults. There are no differences between sexes. The spatial distribution of the plastral pattern is not ordered latitudinally or longitudinally, showing no relationship with gradients of elevation, temperature, or precipitation. This pattern trait lacks of taxonomic significance. The morphometric analysis failed to reveal any character of diagnostic utility in the plastron to support the possibility that these patterns correspond to different sympatric taxa.
Full Text Available Mesoclemmys vanderhaegei is a poorly known freshwater turtle widely distributed in central South America, where it occurs in Argentina, Paraguay, Brazil, and probably Bolivia. It is considered “Near Threatened” by the IUCN Red List and “Data Deficient” by other local lists. Herein, we present new records and data on the reproductive biology of Mesoclemmys vanderhaegei in southeastern Brazil.
Comportamento alimentar e dieta de Phrynops hilarii (Duméril & Bibron em cativeiro (Reptilia, Testudines, Chelidae Feeding behavior and diet of Phrynops hilarii (Duméril & Bibron in captivity (Reptilia, Testudines, Chelidae
Flavio de Barros Molina
Full Text Available Since 1985 the biology and behavior of Phrynops hilarii (Duméril & Bibron, 1835 at São Paulo Zoo is being studied. Feeding behavior is divided in five phases (foraging, approach, capture, dilaceration and ingestion, but not necessarily all of them happen. During phase 1 the food or prey seems to be visually located. During phase 2 the food itens and stationaty preys are approached and examined by olfaction. Moving preys are pursued and there is no olfactory examination. During phase 3 the food is captured by suction. When food is bigger than turtle mouth it is dilacerated by one or both forefeet used alternately (phase 4. Ingestion is accomplished by gradual suction (phase 5. Intra and inter-specific cleptoparasitism was observed. Success in capture and ingestion of food seems not be dependent on species or size of the turtle. Adults, young, and hatchlings of P. hilarii are primarily carnivorous and vegetables were rarely eaten.
Temnocephala brevicornis Monticelli, 1889 is the only species of the genus Temnocephala Blanchard, 1849 reported from chelonians to date. During a survey of the species of Temnocephala extant in southern Uruguay, two new species were found on the chelonian Hydromedusa tectifera Cope, 1869. They are described here as Temnocephala pereirai n. sp. and Temnocephala cuocoloi n. sp. Both resemble T. brevicornis, but differ in the morphometry of the penial stylet, and in qualitative details of the reproductive complex. Temnocephala pereirai n. sp. differs from T. brevicornis by having a massive, cylindrical sphincter in the distal portion of the vagina, and a seminal vesicle that opens into the subpolar to equatorial portion of the contractile vesicle. In addition, the penial stylet in Temnocephala pereirai n. sp. is large in relation to body size, straight and more slender, having the distal portion of its shaft slightly sinuous, and a smaller introvert equipped with about 16 distal crowns of smaller spines. Temnocephala cuocoloi n. sp. is most similar to T. brevicornis, but differs by having a smaller, curved penial stylet that has a smaller introvert in relation to stylet size, with about 10 distal crowns of smaller spines. A key to the species of the Temnocephala from chelonians is provided. This study supports the validity of the following characters previously proposed for the taxonomy of the genus Temnocephala: the shape of the sphincters in the female reproductive system, the shape of the penial stylet, and the number, size, and position of spines in the introvert.
Adriano Lima Silveira
Full Text Available Mesoclemmys tuberculata is a turtle species that is distributed in northeastern Brazil, recorded mainly in the Caatinga and at some localities in the Atlantic Forest and Cerrado. In this paper we report the first species record in an area of Cerrado of Minas Gerais state, and it is the only known state record in a specific location. During field sampling, a specimen of M. tuberculata was collected in the municipality of João Pinheiro, northwest state, in a Cerrado nuclear area in the São Francisco river basin. The locality of this record is the southern and western limits of M. tuberculata’s known distribution, as well as the most inland locality of species record in the Cerrado biome.
Piña, Carlos I.; Argarañaz, Bettina
Nuestro objetivo es establecer los efectos de la temperatura de incubación sobre diferentes aspectos de la ontogenia y la supervivencia de crías de P. hilarii. En el presente trabajo respondemos las siguientes preguntas: 1) ¿afecta la temperatura de incubación al período de incubación de P. hilarii?; 2) ¿cuál es la menor temperatura a la cual existe desarrollo embrionario de P. hilarii?; 3) ¿varía el éxito de eclosión de P. hilarii en relación a la temperatura a la cual se incuban los huevos?...
Piña, Carlos I.
Full Text Available Nuestro objetivo es establecer los efectos de la temperatura de incubación sobre diferentes aspectos de la ontogenia y la supervivencia de crías de P. hilarii. En el presente trabajo respondemos las siguientes preguntas: 1 ¿afecta la temperatura de incubación al período de incubación de P. hilarii?; 2 ¿cuál es la menor temperatura a la cual existe desarrollo embrionario de P. hilarii?; 3 ¿varía el éxito de eclosión de P. hilarii en relación a la temperatura a la cual se incuban los huevos?; 4 ¿está la supervivencia de P. hilarii hasta el año de edad relacionada a la temperatura de incubación?
Guillon, J.-M.; Guéry, L.; Hulin, V.; Girondot, M.
Turtles (Testudines) form a monophyletic group with a highly distinctive body plan. The taxonomy and phylogeny of turtles are still under discussion, at least for some clades. Whereas in most previous studies, only a few species or genera were considered, we here use an extensive compilation of DNA
The purpose of this study was to evaluate the accuracy of hand-held refractometer in determining plasma protein concentrations in South American snake-necked turtle (Hydromedusa tectifera) as compared with the standard biuret method. The results indicated that plasma protein values may be accurately determined in snake- necked turtle with a hand-held refractometer.
Yherson Franchesco Molina Henao; Guillermo Barreto; Alan Giraldo
Rhinoclemmys nasuta (Testudines:Geoemydidae) is considered an almost endemic specie to Colombia and the most primitive species of Rhynoclemmys. However, it is classified data deficient by IUCN because the available information is not enough to make a direct or indirect assessment of its extinction risk. Here, we describe the implementation of the method to analyze the mitochondrial DNA control sequence (mtDNA) of R. nasuta in order to generate tools for future studies in systematics and popul...
Diógenes de Queiroz Dias
Full Text Available Background. Phrynops geoffroanus is a small turtle that inhabits lakes, rivers, and streams throughout South America. The body fat of this animal is used as a folk medicine in Brazil for treating illnesses such as sore throats, ear aches, mumps, rheumatism, and arthritis. The present study evaluated the antimicrobial activity of oil extracted from Phrynops geoffroanus (OPG, determined its chemical composition, and discussed the implications of its use in traditional medicine. The OPG was obtained from the ventral region of this turtle using hexane as a solvent. The antimicrobial activity of OPG was tested against standard and multiresistance strains of bacteria and fungi and its composition was determined indirectly by analyzing the methyl esters of the component fatty acids. The OPG presented a clinically relevant antifungal activity against Candida krusei ATCC 6258 (MIC 128 µg/mL. When the OPG was associated with the antibacterial and antifungal drugs, was observed a synergistic effect when associated the OPG with the gentamicin against the strain Pseudomonas aeruginosa 22. Our results indicated that OPG has clinically relevant antifungal activity against C. krusei, and demonstrated synergetic antibacterial activity in combination with commercial antibiotics against Pseudomonas aeruginosa.
Katie L Willis
Full Text Available The position of testudines in vertebrate phylogeny is being re-evaluated. At present, testudine morphological and molecular data conflict when reconstructing phylogenetic relationships. Complicating matters, the ecological niche of stem testudines is ambiguous. To understand how turtles have evolved to hear in different environments, we examined middle ear morphology and scaling in most extant families, as well as some extinct species, using 3-dimensional reconstructions from micro magnetic resonance (MR and submillimeter computed tomography (CT scans. All families of testudines exhibited a similar shape of the bony structure of the middle ear cavity, with the tympanic disk located on the rostrolateral edge of the cavity. Sea Turtles have additional soft tissue that fills the middle ear cavity to varying degrees. When the middle ear cavity is modeled as an air-filled sphere of the same volume resonating in an underwater sound field, the calculated resonances for the volumes of the middle ear cavities largely fell within testudine hearing ranges. Although there were some differences in morphology, there were no statistically significant differences in the scaling of the volume of the bony middle ear cavity with head size among groups when categorized by phylogeny and ecology. Because the cavity is predicted to resonate underwater within the testudine hearing range, the data support the hypothesis of an aquatic origin for testudines, and function of the middle ear cavity in underwater sound detection.
Willis, Katie L; Christensen-Dalsgaard, Jakob; Ketten, Darlene R
a similar shape of the bony structure of the middle ear cavity, with the tympanic disk located on the rostrolateral edge of the cavity. Sea Turtles have additional soft tissue that fills the middle ear cavity to varying degrees. When the middle ear cavity is modeled as an air-filled sphere of the same......The position of testudines in vertebrate phylogeny is being re-evaluated. At present, testudine morphological and molecular data conflict when reconstructing phylogenetic relationships. Complicating matters, the ecological niche of stem testudines is ambiguous. To understand how turtles have...
Yherson Franchesco Molina Henao
Full Text Available Rhinoclemmys nasuta (Testudines:Geoemydidae is considered an almost endemic specie to Colombia and the most primitive species of Rhynoclemmys. However, it is classified data deficient by IUCN because the available information is not enough to make a direct or indirect assessment of its extinction risk. Here, we describe the implementation of the method to analyze the mitochondrial DNA control sequence (mtDNA of R. nasuta in order to generate tools for future studies in systematics and population conservation. Genomic mtDNA was extracted by salting-out from blood samples from Isla Palma and Playa Chucheros (Bahía Málaga - Colombian Pacific Coast. The polymerase reaction chain (PCR was performed using a pair of degenerate primers (reported for Chrysemys picta, Testudines:Emydidae in polyacrylamide gel stained with silver nitrate. Fragments of 800pb were obtained and the sequencing reaction was effective.High homology percentage (> 92 % was established between the obtained sequences with mtDNA sequences from two species of Geoemydidae, Sacalia quadriocellata and Cuora aurocapitata which are deposited in the GenBank. This demonstrate an effective sequencing ofthe mtDNA control region of R. nasuta.IMPLEMENTACIÓN DE LA METODOLOGÍA DE ANÁLISIS DE ADN MITOCONDRIAL EN Rhinoclemmys nasuta (TESTUDINES:GEOEMYDIDAERhinoclemmys nasuta (Testudines: Geoemydidae es considerada una especie casi endémica de Colombia y la más primitiva del género, sin embargo, se encuentra clasificada por la IUCN como deficiente de datos, ya que la información disponible no es suficiente para hacer una evaluación directa o indirecta de su riesgo de extinción. Con el propósito de generar herramientas de análisis poblacional se describe la metodología de secuenciación de la región control del ADN mitocondrial (ADNmt para el análisis genético de poblaciones de Rhinoclemmys nasuta, que permita establecer bases para futuros estudios evolutivos y de conservación. Se
Martinez, Pablo A; Boeris, Juan M; Sánchez, Julieta; Pastori, María C; Bolzán, Alejandro D; Ledesma, Mario A
We describe for the first time the karyotypes of two species of Cryptodiran turtles from Argentina, namely, Trachemys dorbigni (Emydidae) and Chelonoidis (Geochelone) donosobarrosi (Testudinidae). The karyotype of T. dorbigni (2n = 50) consists of 13 pairs of macrochromosomes and 12 pairs of microchromosomes, whereas the karyotype of C. donosobarrosi (2n = 52) consists of 11 pairs of macrochromosomes and 15 pairs of microchromosomes. Fluorescence in situ hybridization (FISH) with a (TTAGGG)n telomeric probe showed that the chromosomes of these species have four telomeric signals, two at each end, indicating that none of the chromosomes of T. dorbigni and C. donosobarrosi are telocentric. The fact that no interstitial telomeric signals were observed after FISH, suggests that interstitial telomeric sequences did not have a major role in the chromosomal evolution of these species. Additional data will be needed to elucidate if interstitial telomeric sequences have a major role in the karyotypic evolution of Testudines.
Caio Henrique Ferreira
Full Text Available Knowledge on chemical components of the post-hatching eggshell of reptiles may provide indicators of the quality of the diet offered to females kept in captivity. Therefore, the objective of this study was to investigate the chemical characteristics of the calcareous layer, as well as the thickness of Podocnemis expansa post-hatching eggshells. Eggshell thickness was 183±1.405 µm. This value is similar to that of the eggs of other Testudines with flexible eggshells. As for the chemical composition, the following percentages were observed: nitrogen 7.983 ± 0.054; crude protein 49.91 ± 0.324; crude fat 0.068 ± 0.002; mineral matter 20.302 ± 0.807; calcium 13.374 ± 0.647; and phosphorus 0.176 ± 0.003. Knowledge on chemical composition of the eggshell may aid the nutrition of P. expansa raised in commercial facilities, once this species is an alternative and promising source of exotic meat.
Learedius learedi Price 1934 (Digenea, Spirorchiidae in Chelonia mydas Linnaeus 1758 (Testudines, Chelonidae in Brazil: case report Learedius learedi Price 1934 (Digenea, Spirorchiidae em Chelonia mydas Linnaeus 1758 (Testudines, Chelonidae no Brasil: relato de caso
Full Text Available This study reports the occurrence of Learedius learedi Price 1934 (Digenea, Spirorchiidae in Chelonia mydas Linnaeus 1758 (Testudines, Chelonidae in Brazil. Eleven animals were included in this study, 54.6 % of them were parasitized. Two hundred and fifty five parasite specimens were recovered from heart, liver, spleen, lungs, kidneys, mesenterium, and body wash. Results contribute to the knowledge about the helminthofauna of marine chelonian and their geographical distribution. This is the first report of L. learedi in the Southwestern Atlantic.Relata-se a ocorrência de Learedius learedi Price 1934 (Digenea, Spirorchiidae em Chelonia mydas Linnaeus 1758 (Testudines, Chelonidae no Brasil. Onze animais foram examinados e destes, 54,6% estavam parasitados. Duzentos e cinqüenta e cinco exemplares de L. learedi foram recuperados de órgãos (coração, fígado, baço, pulmões, rins, mesentério e do lavado corporal dos animais. Os resultados contribuem para o conhecimento da helmintofauna de quelônios marinhos e sua distribuição geográfica. Este é o primeiro registro da ocorrência de L. learedi na região do Atlântico Sul Ocidental.
Reynolds, R.P.; Gotte, S.W.; Ernst, C.H.
The known type specimens of Crocodilia and Testudines in the collection of the Division of Amphibians and Reptiles, National Museum of Natural History, Smithsonian Institution, published through 2006 represent 93 names of taxa. The catalog presents a list of 249 type-specimen records consisting of 39 holotypes, 52 syntypes, 3 lectotypes, 2 neotypes, 132 paratypes, and 21 paralectotypes. The list is arranged alphabetically by family within Crocodilia and Testudines, and alphabetically by genus and species, as described originally within family. Each entry provides both original and current genus and species names, author(s), date of publication, abbreviated type citation, page of original description, and accompanying fi gures and plates (if any), current type status, USNM catalog number, number of specimens, specimen measurement(s), locality, collector, and date collected. Also included for each taxon is the published type locality, type material at other institutions, an etymology, and remarks on corrections or additional data for original type records, changes in type status, and information pertaining to lost, exchanged, or destroyed specimens. An index of scientific names follows the catalog.
Alejandro De La Ossa L
Full Text Available Objetivo. Analizar los comportamientos de alimentación bajo diferentes condiciones de temperatura en un grupo de neonatos de Peltocephalus dumerilianus (Testudines Podocnemididae. Materiales y Métodos. Los individuos experimentales fueron obtenidos mediante incubación en el laboratorio, se dividieron en 5 grupos al azar. Durante 2 meses con tres repeticiones a la semana se hicieron pruebas de temperatura utilizando dentro de las bandejas calentadores eléctricos, con registro continuo de temperatura mediante termómetro digital - 10 a + 60ºC (± 0.1ºC, procurando mantener un ritmo constante de incremento equivalente a 0,25ºC cada 10 minutos, hasta llegar a la temperatura seleccionada, que fue mantenida por 60 minutos en cada ensayo. Las temperaturas seleccionadas para cada ensayo, fueron: 26ºC, 30ºC, 34ºC, 38ºC y ambiente que fue de 26,8ºC en promedio. Fueron evaluados los siguientes parámetros: duración en minutos de la ingesta, tiempo de inicio de comportamientos agonísticos, presencia de dos tipos de despliegues característicos: morder y disputa por alimento. Resultados. En general todos los parámetros fueron significativos, a mayor temperatura el tiempo de ingestión fue mayor, a menor temperatura el volumen de consumo fue menor, a menor temperatura el inicio de los despliegues agonísticos fue mayor y se mantuvo una relación inversamente proporcional. Conclusiones. Los resultados mostraron que existe relación directamente proporcional entre la temperatura ambiental y los procesos de alimentación, además que se relacionaron con aspectos básicos del comportamiento lo cual se manifestó en los despliegues agonísticos observados.
YHERSON FRANCHESCO MOLINA HENAO
Full Text Available Rhinoclemmys nasuta (Testudines: Geoemydidae es considerada una especie casi endémica de Colombia y la más primitiva del género, sin embargo, se encuentra clasificada por la IUCN como deficiente de datos, ya que la información disponible no es suficiente para hacer una evaluación directa o indirecta de su riesgo de extinción. En este trabajo se describe la implementación del método para realizar la secuenciación de la región control del ADN mitocondrial de R. nasuta, con el propósito de generar herramientas técnicas para futuros estudios de evolutivos y de conservación. Se utilizó el método de desalamiento (Salting-out para extraer ADN a muestras sanguíneas procedentes de Isla Palma y Playa Chucheros (Bahía Málaga–Pacífico Colombiano y se utilizó una pareja de cebadores degenerados (reportada para Chrysemys picta Testudines: Emydidae para realizar la amplificación. Se obtuvieron fragmentos de 800pb siendo exitosa la reacción de secuenciación de los amplificados, y se estableció un porcentaje de homología mayor al 92 % entre las secuencias obtenidas y las secuencias de ADNmt de Sacalia quadriocellata (Testudines:Geoemydidae y Cuora aurocapitata (Testudines:Geoemydidae, depositadas en el GeneBank. Este resultado demuestra que el método descrito puede ser una herramienta útil para el estudio de las poblaciones de R. nasuta del Pacífico colombiano, lográndose una efectiva secuenciación de la región control del ADNmt de esta especie.
Diana Pachón C.
Full Text Available Objetivo. Determinar la presencia de microorganismos del género Salmonella sp. en el ambiente acuático de los ejemplares Crocodylus intermedius y testudines en la Estación de Biología Tropical Roberto Franco (EBTRF. Materiales y métodos. En este estudio se utilizó la metodología estándar para aislar e identificar microorganismos del género Salmonella sp., a partir de muestras de agua y sedimento de 52 estanques (nEstanques Crocodylus=25; nEstanques testudines=27; se procedió a serotipificar los aislamientos por el método convencional de Kaufmann-White y se realizaron pruebas de sensibilidad a antimicrobianos por la técnica de Kirby Bauer. Resultados. Se determinó la presencia de Salmonella sp., en un 33% del total de estanques muestreados. El 29% de los aislamientos de Salmonella sp. serotipificados, correspondió al serogrupo B; los serogrupos C, C1, C2 y D1 presentaron menores porcentajes. Con las pruebas de sensibilidad a antimicrobianos se determinó que el 100% de los aislamientos fueron sensibles a norfloxacina. Conclusiones. La ocurrencia de Salmonella sp., en los estanques de la EBTRF fue del 33%, con la mayor presencia del serogrupo B, donde se encuentran especies con características ampliamente zoonóticas. Con los resultados obtenidos es necesario el seguimiento de las normas de bioseguridad establecidas en la estación para el manejo de las poblaciones allí mantenidas y evitar de esa manera la ocurrencia de cuadros zoonóticos.
Full Text Available Se midieron 31 ejemplares de tortuga africana de espolones Geochelone sulcata para la realización de un estudio biométrico. Se consideraron 26 medidas morfológicas para cada individuo. Las tortugas tenían una edad de entre 5 y 34 meses, y ninguna había alcanzado la madurez sexual. Todas ellas habían nacido en cautiverio en La Garita de Alajuela, Costa Rica, donde son una especie introducida. La longitud recta del espaldar fue de 83.1 mm, la anchura recta del espaldar de 68.3 mm y la altura máxima media de 46.2 mm. Todas las medidas estaban correlacionadas entre sí, salvo la longitud de la cola y la distancia cloacal. El mayor coeficiente de alometría positiva correspondió a la variable Peso. El mayor coeficiente de alometría negativa correspondió a la anchura a nivel de los escudos gulares. Todas las variables se agruparon en dos componentes principales, la longitud de la cola y la distancia cloacal en el Factor 2 y el resto en el Factor 1. La falta de correlación de las medidas de la cola así como su inclusión en un factor aparte al resto de la muestra podría responder a un proceso incipiente de diferenciación sexualGross morphometry of young Geochelone sulcata (Testudines: Testudinidae in Costa Rica. The African Spur tortoise, Geochelone sulcata, has been introduced to Costa Rica. A total of 31 tortoises were measured for 26 gross morphometry parameters. All individuals measured were inmature, aged from 5 to 34 months, and were born in captivity in La Garita de Alajuela, Costa Rica. Mean straight carapace length was 83.1 mm, mean straight plastron length was 68.3 mm and mean maximum height was 46.2 mm. All the measurements were correlated, except tail length and cloacal distance. Weight had the highest positive allometry coefficient. All the variables were joined in two Principal Components; tail length and cloacal distance in Factor 2 and the rest of them in Factor 1. Lack of correlation among tail measures and the other
Holroyd, P. A.; Hutchison, J. H.
The earliest Eocene is marked by significant events of global warming: the Paleocene-Eocene Thermal Maximum (PETM) at ~55.8 Ma and two short-lived events (ETM2 or Elmo and H2) approximately 2 Ma later. These environmental changes induced strong responses in the continental biota. Noteworthy changes in North American mid-latitude faunas and floras that are temporally correlated with earliest Eocene warming events include: increased diversity; turnover; and significant range changes, comprising both northward shifts in ranges of North American taxa as well as intercontinental dispersal across Holarctica. Evidence for these biotic changes comes directly from the fossil record and indirectly from phylogeographic analyses of molecular phylogenies of extant biota. To date, the stratigraphic record of biotic change has only been examined for the flora and terrestrial mammals. Data on reptiles and for continental aquatic systems are particularly lacking. In order to assess the impact of climate-mediated faunal change in aquatic systems during early Paleogene warming, we have focused on developing a detailed record of fossil turtles (Testudines) from the Bighorn Basin of Wyoming, where these records can be directly compared to similarly studied mammalian and floral data and to isotopic studies that provide independent proxies of climate change. Using genus-level occurrence data from more than 450 stratigraphically-constrained localities spanning ~2.5 Ma, we calculated first and last appearances, taxonomic richness, and relative abundance as measured by presence-absence (site occupancy). Among turtles, taxonomic richness increased episodically through the earliest Eocene with two new taxa appearing at the PETM, two immediately following it, and two at Biohorizon B, an interval associated with the younger hyperthermals. These new, immigrant taxa eventually comprised 40% of known generic richness. Phylogenetically, the inferred biogeographic source regions are southern North
Clóvis S. Bujes
Full Text Available O Rio Grande do Sul é o estado mais meridional do Brasil, apresentando fauna e flora peculiares associadas às características morfoclimáticas da região. A diversidade de Testudines do Rio Grande do Sul é representada por seis espécies continentais e cinco marinhas. Este estudo apresenta comentários sobre a diversidade de quelônios continentais do Rio Grande do Sul, através de uma compilação de dados publicados e alguns inéditos sobre sua biologia e estado de conservação.
Rücknagel, K P; Reischl, E; Braunitzer, G
The hemoglobins of two turtles (Testudines)--Chrysemys picta bellii (suborder Cryptodira) and Phrynops hilarii (suborder Pleurodira)--were investigated. In both specimens we found two hemoglobin components with two distinct alpha-chains. The alpha-chains of the component HbD of Chrysemys picta bellii and of the component CII of Phyrynops hilarii belong to the alpha D-type, which has so far been reported to occur only in birds. The complete amino-acid sequences of both alpha D-chains are presented. Our further investigations on hemoglobins of other reptiles (Crocodilia, Lacertilia, Serpentes) did not give any evidence for the expression of alpha D-globin genes in the species examined. These findings are discussed with especial reference to the physiology of respiration. It is supposed that alpha D-genes were of certain significance in earlier times. There are findings suggesting that alpha D-genes are embryonic genes with persistent expression in many adult birds and turtles.
Contribuciones al conocimiento sobre la Ecología y distribución geográfica de Phrynops (Batrachemys Dahli; (Testudinata, Pleurodira, Chelidae Contribuciones al conocimiento sobre la Ecología y distribución geográfica de Phrynops (Batrachemys Dahli; (Testudinata, Pleurodira, Chelidae
; this habit became lost after about four months in captivity. Since movements and sounds produce vibrations in the water, this behavior may possibly be connected with some biological function, as e. g., to facilitate the meeting of the two sexes during mating season, or to announce the presence of an individual within a fixed territory, already occupied; the function could also be a combined one. 15. Specimens have been collected only from the type locality, Sincelejo (Bolivar, 200 meters high, but have been later reported by local inhabitants from different places within the same general region (see map. 16. Chelid turtles were formerly supposed to range only from Colombia, south of Cordillera Oriental, to northern Argentina, but now they are know to extend to north-western Colombia close to the shore of the Caribbean Sea. Since fossil shells of Chelus fimbriatus, together with others of a pelomedusid turtle, Podocemis spec., have been found at La Venta (Huila, a site belonging to Honda group of Late Miocene, situated north of Cordillera Oriental, it seems evident, that the range of the Chelidae extended farther north, before the last formation of the Eastern Andes during Pleistocene. Thus, there exists also the possibility that one or several members of this family had migrated as far as that region, actually still occupied by an isolated form, during Terciary. 1. Data on Ecology, Behavior and Geographical Distribution of the chelid turtle, Phrynops (Batrachemys dahli, are reported. 2. The original habitat of the "carranchina" or "cabeza al lado" consisted in ponds and small brooks within the forests, a situation which has gradually changed; actually the entire area around the type locality is transformed into pastures. 3. It is principally carnivorous and feeds on snails, aquatic insects, tadpoles, frogs, small fishes, carrion, but also sometimes on vegetable matter. No endoparasites had been found. 4. A marked dimorphism exists between the two sexes (figs. 2, 4, 5
Jannello, Juan Marcos; Cerda, Ignacio A.; de la Fuente, Marcelo S.
Yaminuechelys is a long-necked chelid turtle whose remains have been recovered from outcrops of the Santonian-Maastrichtian and Danian of South America. With the purpose of providing data about shell sculpturing origin and palaeoecology, the bone histology of several shell elements (including neural, costal, peripheral and plastral plates) of Yaminuechelys is described herein. Histological analysis reveals that Yaminuechelys shares with Chelidae the presence of interwoven structural fibre bundles in the external cortex, and parallel-fibred bone of the internal cortex. The presence of resorption lines in several samples indicates that the particular ornamentation of the external surfaces originated, at least in part, by focalized resorption and new bone deposition. This mechanism for ornamentation origin and maintenance is here described for the first time in a turtle. Compactness of the shell bones is consistent with an aquatic habitat, which supports previous hypothesis based on palaeoenvironmental and morphological data.
Full Text Available Con el objetivo de determinar la flora normal aerobia, cloacal y nasal de la tortuga lora (Lepidochelys olivacea , entre los meses de julio y agosto del 2002,se colectaron muestras bacteriológicas de 45 quelonios aparentemente sanos,durante el desove en Playa Nancite,Parque Nacional Santa Rosa, Costa Rica, a través del uso de hisopos estériles que se introdujeron en la cloaca y en uno de los conductos nasales. De las muestras recolectadas se obtuvieron e identificaron un total de 99 aislamientos, incluyendo 10 grupos de Gram-negativos y 5 de Gram-positivos. De cada tortuga se obtuvo un promedio de 0.7 bacterias de la cloaca y 1.4 de las cavidades nasales. Las bacterias más frecuente halladas fueron Aeromonas spp.(13/45 y Citrobacter freundi (6/45 en la cloaca, y Bacillus spp. (32/45,Staphylococcus aureus (6/45y Corynebacterium spp.(5/45en las cavidades nasales. En este investigación, la flora microbiana de las tortugas lora resultó constituida por microorganismos potencialmente patógenos para el ser humano y las tortugas.Cloacal and nasal bacterial flora of Lepidochelys olivacea (Testudines: Cheloniidae from the North Pacific coast of Costa Rica.The aerobic cloacal and nasal bacterial flora of 45 apparently healthy female olive ridley sea turtles (Lepidochelys olivacea was studied at Nancite nesting beach,in Santa Rosa National Park (Costa Rican North Pacificduring July and August 2002.Bacterial samples were obtained by inserting sterile swabs directly into the cloaca and the nasal cavities of the turtles.Ninety-nine aerobic bacterial isolates, including 10 Gram-negative and 5 Gram-positive bacteria, were recovered.The most common bacteria cultured were Aeromonas spp. (13/45 and Citrobacter freundi (6/45from cloacal samples and Bacillus spp.(32/45, Staphylococcus aureus (6/45and Corynebacterium spp.(5/45from nasal ducts.The results of the present study showed that the aerobic bacterial flora of nesting female olive ridleys was composed of
Actividad reproductiva de Chelonia mydas (Testudines: Cheloniidae en Isla de Aves, Venezuela (2001-2008 Reproductive activity of Chelonia mydas (Testudines: Cheloniidae in Isla de Aves, Venezuela (2001-2008
Full Text Available Isla de Aves, una isla a 650km de La Guaira, Venezuela, protegida como Refugio de Fauna Silvestre, constituye el segundo sitio de mayor anidación de la tortuga verde (Chelonia mydas (Linnaeus 1758 en el Caribe. El seguimiento de la población comenzó en 1972 y de manera más continua desde 1978. Los datos históricos indican que la captura de hembras en la isla, afectó severamente la población hasta 1978, cuando fue construida una base científico-naval. Durante las temporadas de anidación entre 2001-2008 con excepción de 2003 y 2004, las hembras fueron marcadas con placas metálicas y medidas. Asimismo, se muestreó durante 458 noches, en donde se observaron 5 154 eventos, con un máximo de 53 por noche. Los posibles eventos no observados fueron calculados ajustando la distribución temporal de eventos observados a una curva normal. El total de eventos estimados varió de =637.1±106.6 en 2001 a =2 853±42.5 en 2008 (ANOVA F(6.5gl=60.37, pReproductive activity of Chelonia mydas (Testudines: Cheloniidae in Isla de Aves, Venezuela (2001-2008. The second major nesting-site for green turtles in the Caribbean is Isla de Aves, an island protected as a wildlife refuge since 1972, located at 650km Northeast from La Guaira, Venezuela. In this island, the nesting population monitoring started in 1972 and in a more continuous way after 1978, when a Scientific-Naval Station was established and scientific observations started. Since historical data show that female captures had severely affected population levels in this island before 1978, this study aim to describe recent reproductive activities. For this, during the nesting seasons of 2001-2002 and 2005-2008, nesting females were measured and tagged using metal flipper tags. A total of 458 nights were sampled observing 5 154 female emergences, with a maximum of 53 in a single night. Non-observed emergences were calculated fitting the temporal distribution of observed emergences to a normal curve
Aislamiento e identificación de microorganismos entéricos en muestras ambientales y cloacales en crocodylus intermedius y testudines de la estación de biología tropical roberto franco en villavicencio, colombia
Pachón, D. A.; A. P. Pulido; Moreno, C.A.
Los microorganismos entéricos han sido frecuentemente reportados como patógenos en mamíferos, aves, peces, reptiles y humanos, a pesar de hacer parte de su flora normal intestinal. La Estación de Biología Tropical Roberto Franco (EBTRF), lidera el programa de recuperación del Caimán Llanero ( Crocodylus intermedius ), que se encuentra en inminente peligro de extinción; adicionalmente cuenta con una colección viva de Testudines que comprende más de 20 especies. Con el fin de determinar la p...
Supernumerary epidermal shields and carapace variation in Orbigny's slider turtles, Trachemys dorbigni (Testudines, Emydidae Escudos epidérmicos supernumerários e variação na carapaça na tartaruga-tigre-d'água, Trachemys dorbigni (Testudines, Emydidae
Clóvis S. Bujes
Full Text Available The epidermal plates of the carapace and plastron of 51 adults (38 females and 13 males, 07 immature individuals, and 46 hatchlings of the freshwater turtle Trachemys dorbigni (Durémil & Bibron, 1835, originated from the delta of Rio Jacuí region, Rio Grande do Sul state, Brazil, were examined. The results showed that 7.7% of males, 10.52% of females, 14.28% of immature individuals, and 6.52% of the hatchlings presented a kind of anomaly on the shell, as well as a presence of supernumerary epidermal shields. Although the modification in the number of epidermal shields presents a high frequency in Testudines, these are the first descriptions of the variation in the pattern of carapacial scutation in eleven individuals from a population of T. dorbigni. The association of several environmental factors acting on the embryonic development of the individual may be responsible for the alteration of the pattern of carapacial scutation in this species.Os escudos epidérmicos da carapaça e do plastrão de 51 adultos (38 fêmeas e 13 machos, 07 imaturos e 46 filhotes da tartaruga de água doce Trachemys dorbigni (Durémil & Bibron, 1835, procedentes da região do delta do Rio Jacuí, Rio Grande do Sul, Brasil, foram examinados. Desta análise, 7,7% dos machos, 10,52% das fêmeas, 14,28% dos imaturos e 6,52% dos filhotes apresentaram algum tipo de anomalia no casco, bem como presença de escudos epidérmicos supernumerários. Embora a alteração no número dos escudos epidérmicos seja relativamente freqüente em Testudines, estas são as primeiras descrições de variação no padrão de escutelação em onze indivíduos de uma população de T. dorbigni. A associação de diferentes fatores ambientais, interagindo sobre o desenvolvimento embrionário do indivíduo, parece ser a responsável pela alteração do padrão de escutelação nessa espécie.
Ontogeny of the cranial bones of the giant amazon river turtle Podocnemis expansa Schweigger, 1812 (Testudines, Podocnemididae - doi: 10.4025/actascibiolsci.v32i2.5777 Ontogeny of the cranial bones of the giant amazon river turtle Podocnemis expansa Schweigger, 1812 (Testudines, Podocnemididae - doi: 10.4025/actascibiolsci.v32i2.5777
Fabiano Campos Lima
Full Text Available In order to determine the normal stages of formation in the sequence of ossification of the cranium of Podocnemis expansa in its various stages of development, embryos were collected starting on the 18th day of natural incubation and were subjected to bone diaphanization and staining. In the neurocranium, the basisphenoid and basioccipital bones present ossification centers in stage 19, the supraoccipital and opisthotic in stage 20, the exoccipital in stage 21, and lastly the prooptic in stage 24. Dermatocranium: the squamosal, pterygoid and maxilla are the first elements to begin the ossification process, which occurs in stage 16. However, ossification centers begin to appear in stage 17 in most of these bone elements, i.e., the frontal, jugal, postorbital, parietal, premaxilla and prefrontal, followed by the palatine and quadratojugal in stage 19 and lastly by the vomer in stage 25. The quadrate bone of the splanchnocranium ossifies in stage 23. The mandible and hyoid apparatus, the dentary, coronoid and supra-angular, show ossification centers in stage 16 and the branchial horn I in stage 17. The sequence and synchronization of ossification in P. expansa show similarities as well as differences when compared with other species of Testudines.In order to determine the normal stages of formation in the sequence of ossification of the cranium of Podocnemis expansa in its various stages of development, embryos were collected starting on the 18th day of natural incubation and were subjected to bone diaphanization and staining. In the neurocranium, the basisphenoid and basioccipital bones present ossification centers in stage 19, the supraoccipital and opisthotic in stage 20, the exoccipital in stage 21, and lastly the prooptic in stage 24. Dermatocranium: the squamosal, pterygoid and maxilla are the first elements to begin the ossification process, which occurs in stage 16. However, ossification centers begin to appear in stage 17 in most of these
Development of a quantitative PCR for rapid and sensitive diagnosis of an intranuclear coccidian parasite in Testudines (TINC), and detection in the critically endangered Arakan forest turtle (Heosemys depressa).
Alvarez, W Alexander; Gibbons, Paul M; Rivera, Sam; Archer, Linda L; Childress, April L; Wellehan, James F X
The intranuclear coccidian parasite of Testudines (TINC) is responsible for significant disease in turtles and tortoises causing high mortality and affecting several threatened species. Diagnostic testing has been limited to relatively labor intensive and expensive pan-coccidial PCR and sequencing techniques. A qPCR assay targeting a specific and conserved region of TINC 18S rRNA was designed. The qPCR reaction was run on samples known to be TINC positive and the results were consistent and analytically specific. The assay was able to detect as little as 10 copies of target DNA in a sample. Testing of soil and invertebrates was negative and did not provide any further insights into life cycles. This assay was used to identify TINC in a novel host species, the critically endangered Arakan forest turtle (Heosemys depressa).
Full Text Available RESUMO: Este estudo objetivou avaliar a biometria corporal e o perfil hematológico de Trachemys scripta elegans (N=28 e de Trachemys dorbignyi (N=22 criadas em cativeiro na região do submédio do Vale do São Francisco, semiárido nordestino brasileiro, visando estabelecer valores sanguíneos básicos de saúde e gerar dados úteis na fisiologia comparativa de Testudines. Após 120 dias de adaptação e jejum de 24 horas, 2,5 mL de sangue foram coletados do seio occipital dorsal e depositados em tubo com heparina sódica para a avaliação, na sequência, dos níveis hematologicos. A contagem total de eritrócitos (CTE e global de leucócitos (CGL foi realizada em câmara de Neubauer; a dosagem de hemoglobina (HGB pelo método da método da cianometahemoglobina e o hematócrito (HCT através da técnica do microhematócrito. A partir da CTE estabeleceram-se matematicamente os índices hematimétricos. A biometria corporal também foi avaliada: a massa corporal (MC, g; b dimensões máximas da carapaça [comprimento (CMC, cm e largura (LMC, cm];c dimensões máximas do plastrão [comprimento (CMP, cm e largura (LMP, cm]; d comprimento total da cauda (CTC, cm; e comprimento linear da base da cauda ao orifício cloacal (CprC, cm; f comprimento linear do orifício cloacal a extremidade da cauda(CpoC, cm. T. scripta elegans apresentaram valores maiores (P 0,05 entre as espécies. Os resultados demostram que a maior parte da variação observada entre T. scripta elegans e T. dorbignyi é explicada pelas variáveis biométricas e que algumas correlações hematológicas caracterizam diferenças interespecíficas. Conclui-se que os resultados lançam luz sobre valores de referência para estas espécies mantidas em cativeiro na região do semiárido e servem como um modelo para a fisiologia comparativa intra e interespécies.
J.F. Arango Ospina
Full Text Available Presentamos el caso de un paciente de 2 años de edad, conocido en su pueblo, como "el niño tortuga (testudines" debido a la gran similitud con dicho reptil. Desde su nacimiento presentó nevus melanocítico gigante localizado en región torácica posterior, región lumbosacra y lesiones satélites en extremidades y abdomen, de 50 por 40cm de diámetro y color café oscuro; la piel en las zonas afectadas es gruesa con grandes surcos de aspecto corrugado (cerebriforme. El nombre dado al niño se basa en la similitud de sus lesiones con la morfología de una tortuga, ya que el caparazón cubre la zona superior, inferior y lateral del cuerpo de este animal, de la misma forma que el nevus gigante lo hacía en el caso de nuestro paciente. Este caparazón está formado por placas óseas revestidas de placas corneas, que se asemejan a los surcos de aspecto corrugado que conforman el nevus melanocítico gigante. Los estudios clínicos practicados revelaron compromiso cardiovascular, hepático y esplénico, así como una desfavorable evolución y pronostico del cuadro clínico, por lo que esta patología, específicamente para este niño y de acuerdo a la junta Médico-Quirúrgica y valoraciones, fue considerada inoperable.We present the case of a 2 years-old patient, known in his town as "turtle boy"(testudines due to his great simility with this reptile because of his giant congenital melanocytic nevus, located in the posterior and lateral aspect of the chest and in smaller proportion in abdomen and extremities, of 50 by 40cm and dark brown colour. The skin in this zone is thick with great furrows and corrugated aspect. He was called "turtle boy" based on the morphology of the turtle, its shell covers the superior, inferior and lateral zone like in our patient's case. It is formed by bone layers covered by cornea layers, which resemble the furrows and corrugated aspect of the giant congenital melanocytic nevus. Medical investigation reveals
María Rondón Médicci
Full Text Available En Playa Cipara, Península de Paria, Venezuela, se evaluó el impacto de la luz artificial sobre la anidación de la tortuga cardón. Se estimó el número de anidaciones y su distribución espacial a lo largo de la playa entre los años sin y con iluminación artificial y entre segmentos de playa iluminada y oscura. Se hicieron entrevistas a los residentes para conocer su percepción sobre el impacto de la luz artificial hacia las tortugas marinas. Entre el 2000 y 2005 se registraron 1 217 salidas de tortuga cardón; con 1 056 nidos. El número de nidos con huevos dependió significativamente del año (p=0.035 al igual que el número de nidos totales (p=0.015. En los años previos a la electricidad (2000-2003 se observaron 743 salidas, de las cuales 661 con nido y 374 nidadas confirmadas. En los dos años (2004-2005 con iluminación artificial, se contaron 474 salidas con 395 nidos y 232 nidadas. La proporción de salidas con construcción de nido disminuyó significativamente (p=0.005 en los años con luz eléctrica, pero no varió el éxito de desove (p=0.402. No se encontró diferencia significativa entre el número de salidas por metro de playa en los sectores oscuros y los iluminados (p=0.244, ni entre el número de nidos construidos (p=0.379, ni entre las anidaciones con desove (p=0.516. Tampoco en la proporción de las salidas totales que constituyeron anidaciones (p=0.067 entre los sectores iluminados y oscuros, ni en la proporción de nidos exitosos (p=0.833. El volumen medio de arena por metro de playa fue mayor en La Peña, Cipara y La Remate y menor en Varadero (pImpact of artificial light on nesting in the leatherback turtle Dermochelys coriacea (Testudines: Dermochelyidae at Cipara beach, Venezuela. The number of Leatherback turtle nests and their spatial distribution was compared between years with and without artificial light, and between dark and lighted beach segments, in Cipara Beach, Paria Peninsula, Venezuela. Residents
María Isabel Castro-González
Full Text Available Por su potencial como fuente alimenticia, se analizó la composición química de huevo de Lepidochelys olivácea en La Escobilla, Oaxaca, México. El Instituto de Ciencias del Mar y Limnología de la Universidad Nacional Autónoma de México proporcionó 250 muestras de huevo liofilizado de 25 tortugas, que se analizaron siguiendo métodos oficiales para humedad, cenizas, proteína, grasa, lípidos totales(LT y análisis microbiológicos; además de ácidos grasos(AG por cromatografía de gases, aminoácidos(AA, vitaminas y colesterol(col por HPLC. Los resultados fueron los siguientes: (g/100g humedad(4.7, cenizas(3.8, proteína(53.7 y grasa(47.4. AA esenciales (g aa/100g Proteína: Ile (4.4, Lys(6.6, Leu(7.4, Met+Cys (8.8, Phe+Tyr (10.8; retinol (340μg/100g, colecalciferol (5.9 μg/100g, tocoferol (8.6, tiamina (0.3 y riboflavina (1.1 (mg/100g. Y los de (LT, (AG y (Col se concentraron en tres grupos por peso de tortuga: (LT (44.3-48.7-49.1g/100g, (Col (518.4-522.5-728.7 mg/100g. Entonces se identificaron 17 AGSaturados, 8 AGMonoinsaturados y 11 AGPoliinsaturados. Los AGS más abundantes (mg/100g: C16:0 (485-1263, AGM: C16:1 (456-716, C18:1n-9c (904- 1754 y AGP: C20:4n-6 (105-217, EPA (48-103 y HA (97-189. También, existió diferencia significativa (Fisher, pChemical composition of eggs of the Olive Ridley Lepidochelys olivacea (Testudines: Cheloniidae and it’s potential as a food source. The Olive Ridley is a worldwide distributed species with high nesting production per season, and in La Escobilla Oaxaca, México, there is a 70% of non-hatched eggs that are lost. In order to evaluate their potential use as a source for human and animal food products, their chemical composition was analyzed. Lyophilized egg samples from 25 turtles were obtained and were analyzed following the analytical methods for fatty acids, protein, fat, ash, moisture, amino acids, vitamins, cholesterol and microbiological agents. The analytical composition obtained
Aislamiento e identificación de microorganismos entéricos en muestras ambientales y cloacales en Crocodylus intermedius y Testudines de la Estación de Biología Tropical Roberto Franco en Villav icencio, Colombia
D. A. Pachón
Full Text Available Los microorganismos entéricos han sido frecuentemente reportados como patógenos en mamíferos, aves, peces, reptiles y humanos, a pesar de hacer parte de su flora normal intestinal. La Estación de Biología Tropical Roberto Franco (EBTRF, lidera el programa de recuperación del Caimán Llanero ( Crocodylus intermedius , que se encuentra en inminente peligro de extinción; adicionalmente cuenta con una colección viva de Testudines que comprende más de 20 especies. Con el fin de determinar la presencia de potenciales enteropatógenos en el hábitat de los ejemplares, se obtuvieron 129 muestras ambientales y cloacales de las especies allí encontradas; se utilizó el medio de cultivo CHROMagar Orientación BD® para realizar los aislamientos y la identificación microbiológica. Los resultados muestran una mayor presentación de flora gram negativa predominando microorganismos de los géneros Escherichia coli (28%, Klebsiella sp (26%, Salmonella sp. (6%, Proteus sp (3% y Citrobacter sp. (1% Sin embargo, microorganismos del género Enterococcus sp. (gram positivo, fueron hallados en un mayor porcentaje (31% en todas las muestras sin importar el origen de las mismas. Conscientes del riesgo que implica el aislamiento de microorganismos entéricos que pueden presentar un carácter zoonótico, se dio inicio a la implementación de un manual de bioseguridad para la Estación con el fin disminuir el riesgo para la población humana y animal.
debe cambiar a: C. mydas: por encima de 93.0cm y E. imbricata por encima de 79.0cm.Gonadic histology and phenotypical maturation criteria in the marine turtles Chelonia mydas and Eretmochelys imbricata (Testudines: Chelonidae from Cuba. Gonad maturity is usually evaluated through macroscopic analysis of the gonads. In sea turtles, the maturation stages are associated with body size, depending on the studied marine stock. Fishermen classify turtles bigger than 65.0cm as sexually mature. If they have secondary sex characters they are recorded as breeding males. We compared body size with macroscopic and microscopic gonad characteristics in two Cuban turtles. Eighteen individuals of C. mydas and twenty of E. imbricata was obtained from the legal fishery stock of Jardines del Rey Archipelago (Cuba, from October 2005 and 2006. In males, breeding condition (maximum spermiogenesis was checked by histological analysis of the testes. In females, sexual maturity was identified by the presence of vitellogenic follicles or ovarian corpora. Most males were immature (C. mydas: 79.0cm; E. imbricata: 73.1±4.9cm, n=3 and lacked secondary sex characters. Some E. imbricata without a developed penis were in spermatogenic stages II to IV (i.e. pubescent. Most females were immature (C. mydas: 79.6±7.7cm, n=17; E. imbricata: 69.0±7.1cm, n=16; i.e.prepubescent and pubescent. The prepubescent females had ovaries with previtellogenic follicles near 1.0mm in a compact and yellowish stroma. The pubescent females had ovaries with previtellogenic follicles between 2.0 and 3.0mm. The stroma was more loosened and irrigated than in prepubescent turtles. The finding of spermatogenic activity in pubescent males indicates asynchrony between testicular and penial development in E. imbricata. The current phenotypical approach used by fishermen is not enough to determine sexual maturation in these turtles. The minimal size tentatively should be changed to: C. mydas: above 93.0cm and E. imbricata above 79
Martins, G S; Alevi, K C C; Azeredo-Oliveira, M T V; Bonini-Domingos, C R
The hematology of wild and captive animals is essential for obtaining details about species and represents a simple method of diagnosing disease and determining prognosis. Few studies have described the morphology of chelonian blood cells, which are more common in sea and freshwater turtle species. Thus, in order to further our understanding and recognition of different chelonian cells types, the present study aimed to describe blood cells from the two species of Brazilian tortoises, Chelonoidis carbonarius and C. denticulatus. Cytochemical analysis of tortoise blood tissue with Panótico®, made it possible to describe all the of the chelonian cell types (with the exception of thrombocytes): erythrocytes, agranular leukocytes (monocytes and lymphocytes), and granular leukocytes (eosinophils, heterophils, basophils, and azurophils). These data are of high importance for establishing hematological profiles of Brazilian tortoises and reptiles. Therefore, based on our results and on comparative analyses with data from the literature for other reptile species, we can conclude that the blood cells described for Brazilian tortoises are found in all species of reptiles that have been analyzed thus far, and may be characterized and used as a comparative parameter between different groups to evaluate the health status of these animals.
Marcela dos S. Magalhães
Full Text Available This study characterized the morphology of the digestive tube of five species of sea turtles. We used specimens found dead along the coast of the state Rio Grande do Norte, as well as specimens accidentally killed as a result of pelagic longline fishing. Nineteen animals of the following species were analyzed: Chelonia mydas (Linnaeus, 1758 (n = 9, Lepidochelys olivacea (Eschscholtz, 1829 (n = 6, Caretta caretta (Linnaeus, 1758 (n = 2, Eretmochelys imbricata (Linnaeus, 1766 (n = 1 and Dermochelys coriacea (Vandelli, 1761 (n = 1. After opening the plastron, we removed the digestive organs and described the external and internal morphology of each organ. The esophagus of all species had pointed papillae on the mucosa. The stomach varied in shape among species. Differences were found in the mucosa of the small intestine. It was reticular in the duodenum, and longitudinal rectilinear in the jejunum/ileum. In all species an alternation of saccular and narrow regions was observed in the large intestine. The exception was D. coriacea, in which the mucosa of the entire large intestine had irregularly distributed folds. The pattern of the esophagus was the same in all species. The morphology of the stomach differed among species, and these differences reflect their diets. In addition, the distribution pattern of the folds on the mucosa of the small intestine varied between regions of the intestine and among species.
Derocco, Natacha N.
Full Text Available Phrynops hilarii presenta una amplia distribución en la región noreste de la República Argentina, en las provincias de Buenos Aires, Chaco, Córdoba, Corrientes, Entre Ríos, Formosa, Misiones, Santa Fe, Santiago del Estero (Cabrera, 1998, incluyendo poblaciones establecidas por antropocoria en Mendoza (Richard, 1999.
Souza Franco L.
Full Text Available Hydromedusa maximiliani is a vulnerable neotropical freshwater turtle endemic to mountainous regions of the Atlantic rainforest in southeastern Brazil. Random amplified polymorphic DNA (RAPD was used to estimate the gene flow and dispersal for individuals inhabiting rivers and streams within a drainage. Nine primers generated 27 scoreable bands, of which 9 (33% were polymorphic and produced 12 RAPD phenotypes. The gene flow estimates (Nm among turtles inhabiting different rivers and streams were variable, ranging from 0.09 to 3.00 (mean: 0.60. For some loci, the rates of gene flow could offset population differentiation (Nm > 1, whereas for others random genetic drift could result in population divergence (Nm < 1. Since the genetic variation of this turtle seems to be structured according to the natural hierarchical system of rivers and streams within drainages, management programs involving translocations between different regions across the geographical range of H. maximiliani should be viewed with caution.
Full Text Available 1. The first exact data about reproduction of the Colombian Painted Turtle, Pseudemys scripta callirostris, are presented, based on studies carried out on 75 adults, 89 hatchlings and 276 eggs.2. There exists a marked sexual dimorphism which comprises the size of the shell, length of tail, and shape of shell and head. The Carapace length, taken in straight line, is up to 300.0 mm in ♀♀ and 252.0 mm in ♂ ♂, but generally less.3. The mating season takes place between September and December.4. Copulation takes place in quiet but deep water and lasts for about 2-3 minutes.5. Only the hind legs are used to excavate the nest; the entire process (excavation, egg laying and covering of the hole lasts between one hour, twenty minutes and four hours, twenty five minutes aproximately. (Figs. 1-2.6. The flask-shaped nesting hole is up to 180.0 mm deep, 110.0 mm wide at the entrance and 130.0 mm wide at the bottom. (Table 1.7. There are from 9-30 eggs to be found in a single nest which are deposited in 2-3-4 layers; the first eggs (last layer are found in 67.0 mm-10.0mm depth. (Table 3.8. The oblong, soft-shelled eggs are pinkish-white when recently laid, they show, moreover, a shallow concavity about 19.0 mm-21.0 mm long. In contrast, those already containing embryos have a less flexible shell, no concavity and are white. (Fig. 3-4.9. The measurements and weight of 152 eggs comprises from 41.0:26.0 mm to 27.0:21.0 mm, and between 15 g, 670 mg and 5 g, 170 mg respectively. (Table 4.10. The egg-laying season takes place principally between late December and late April; according to information there is a second one in August but to a lesser degree; dissected ♀♀ contained both eggs and numerous ovules. (Table 2.11. The incubation period varies between 69 and 92 days. (Table 6.12. Hatchlings are born from late april to early June. 13. The Carapace length ranges from 39.0 mm to 29.5 mm, and the weight varies between 13 g, 300 mg and 7 g, 60 mg. (Tables 7,8,9; fig. 5.14. The egg caruncle normally disappears after 5-9 days, but in some cases it is already vestigial or even worn off at the day of hatching.15. Growing rates of eight specimens between 1966 and 1971 are presented in table 7; however, these rates under natural conditions within the original habitat are still unknown.16. Either natural enemies or, and mainly, human activities cause great losses every year. The eggs are eaten by certain mammals, birds and reptiles (Tupinambis nigropunctatus ; hatchlings are sold in great quantities for exportation to pet shops, and adults are sold at the markets during the Holy Week, mostly ♀♀ containing eggs and stored since January.17. The establishment of breeding stations and areas totally protected for breeding under natural conditions are recommended.1.Se presentan los primeros datos exactos sobre la reproducción de la "Icotea", Pseudemys scripta callirostris, una tortuga de agua dulce¡ endémica en Colombia.2. Se estudiaron 75 ejemplares adultos de ambos sexos, 89 crías y 276 huevos.3. Existe un dimorfismo sexual marcado.4. La época de celos se efectúa entre septiembre y diciemhre.5. La cópula se efectúa en aguas mansas y profundas y dura unos 2·3 minutos.6. Para la excavación de los nidos se usan solamente las extremidades posteriores; la durución del proceso total de excavación, postura de huevos y tapamiento de hueco es variada; en dos casos duró una hora y veinte minutos y cuatro horas veinticinco minutos respectivamente, (Figs. 1-2.7. El nido tiene la forma de una botella redondeada en el centro, y sus dimensiones comprenden: Profurididad hasta 180.0 mm.; ancho (entrada hasta 110.0 mm., y ancho (fondo hasta 130.0 mm. (tabla 1.8. La cantidad de huevos en un solo nido varía entre 9 y 25; en varios casos se encontraron hasta 30, según informes. (Figs. 3-4.9. Estan depositados en 2·3·4 capas; los primeros huevos (última capa se encuentran en una profundidad de 67.0 mm. a 10.0 mm. (tabla 3.10. Son de forma oblonga; en los recién puestos la cáscara es blanda y su color blancuzco-rosado ; tienen, además, una escotadura poco profunda de 19.0 mm-21.0 mm; en cambio, la cáscara de huevos con embriones es más dura, no tiene escotadura y su color es blanco, (Figs. 3.4.11. Las dimensiones de 152 huevos varian entre 41.0:26.0 mm y 27.0:21.0 mm; el peso comprende de 15 g, 670 mg a 5g, 170 mg. (Tabla 4.12. Se efectúan una o dos posturas al año; en 4 ♀♀ disecadas se encontraron tanto huevos como óvulos de diámetro variado. (Tabla 2.13. La época principal de anidación se extiende de fines de diciembre a fines de abril; según informes, la segunda se efectúa en agosto.14. El periodo de incubación varía entre 69 y 92 dias. (Tabla 6.15. La cría nace de fines de abril a principios de junio.16. La longitud del Carapax cOmprende de 39.0 mm a 29.5 mm, y el peso fluctúa entre 13 g, 300 mg y 7 g, 60 mg. (Tablas 7, 8, 9; fig. 5.17. El Ovirruptor, una pequeña protuberancia epidérmica situada en la punta del hocico, funciona para cortar tanto las membranas embriológicas como la cáscara del huevo; desaparece despues de 5-9 días por lo general.18. Los datos sobre el crecimiento de ocho ejemplares entre 1966 y 1971 estan presentados en la tabla 7.19. Tanto diferentes enemigos naturales como, especialmente, las actividades humanas causan anualmente grandes perdidas entre los huevos y ejemplares recién nacidos y adultos.20. Se recomienda el establecimiento de criaderos debidamente mantenidos, vigilados y orientados por un personal científico competente con fines de proteger más efectivamente a un elemento faunístico endémico.
Adam M. Yates
Full Text Available The new species Chelodina (Chelodina murrayi is described from the late Miocene Alcoota Local Fauna of central Australia, in the Northern Territory. The new species is based on shell fragments and can be diagnosed by a ventrally reflexed anterior margin of the plastron, a ventrally narrowed cervical scute and strongly dorsally curved margins of the carapace extending from approximately peripheral two to peripheral nine or ten as well as by a unique combination of characters. Within Chelodina the new species is part of the nominal subgenus and within that subgenus it is most closely related to the Chelodina (Chelodina novaeguineae species group. This is not only the oldest record but also the most southerly occurrence of this species group.
Walter G. Joyce
Full Text Available Background Bothremydidae is a clade of extinct pleurodiran turtles known from the Cretaceous to Paleogene of Africa, Europe, India, Madagascar, and North and South America. The group is most diverse during the Late Cretaceous to Paleogene of Africa. Little is known, however, about the early evolution of the group. Methods We here figure and describe a fossil turtle from early Late Cretaceous deposits exposed at MacFarlane Mine in Cedar Canyon, southwestern Utah, USA. The sediments associated with the new turtle are utilized to infer its stratigraphic provenience and the depositional settings in which it was deposited. The fossil is compared to previously described fossil pleurodires, integrated into a modified phylogenetic analysis of pelomedusoid turtles, and the biogeography of bothremydid turtles is reassessed. In light of the novel phylogenetic hypotheses, six previously established taxon names are converted to phylogenetically defined clade names to aid communication. Results The new fossil turtle can be inferred with confidence to have originated from a brackish water facies within the late Cenomanian Culver Coal Zone of the Naturita Formation. The fossil can be distinguished from all other previously described pleurodires and is therefore designated as a new taxon, Paiutemys tibert gen. et. sp. nov. Phylogenetic analysis places the new taxon as sister to the European Polysternon provinciale, Foxemys trabanti and Foxemys mechinorum at the base of Bothremydinae. Biogeographic analysis suggests that bothremydids originated as continental turtles in Gondwana, but that bothremydines adapted to near-shore marine conditions and therefore should be seen as having a circum-Atlantic distribution.
Li, Wei; Zhao, Jian; Shi, Yan; Xiao, Feng-Fang; Zhang, Xin-Cheng; Zhu, Xin-Ping
The complete mitochondrial genome of the Vietnamese three-striped box turtle (Cuora cyclornata) was first determined in this study. It was a circular molecule of 16,594 bp in length, consisting of 37 genes typically found in other vertebrates. The AT content of the overall base composition of the whole mitogenome was 60.39%, while the control region was 70.23%. Two ETAS and 4 CSBs were identified, while a remarkable feature was found in the control region: a large number of (TTATTATA)10 direct tandem repeats followed by (TTATA)n (n=10, 8 and 1), which were spaced into three domains by (TA)n (n=1, 1 and 2). The sequence information could play an important role in the study of phylogenetic relationships in turtles and preservation of genetic resources for helping conservation of the endangered species.
Petzold, Alice; Vargas-Ramírez, Mario; Kehlmaier, Christian; Vamberger, Melita; Branch, William R; Preez, Louis Du; Hofmeyr, Margaretha D; Meyer, Leon; Schleicher, Alfred; Siroký, Pavel; Fritz, Uwe
Using nearly range-wide sampling, we analyze up to 1848 bp of mitochondrial DNA of 183 helmeted terrapins and identify a minimum of 12 deeply divergent species-level clades. Uncorrected p distances of these clades equal or clearly exceed those between the currently recognized species of Pelusios, the genus most closely related to Pelomedusa. We correlate genetic discontinuities of Pelomedusa with data on morphology and endoparasites and describe six new Pelomedusa species. Moreover, we restrict the name Pelomedusa subrufa (Bonnaterre, 1789) to one genetic lineage and resurrect three further species from its synonymy, namely P. galeata (Schoepff, 1792), P. gehafie (Rüppell, 1835), and P. olivacea (Schweigger, 1812). In addition to these ten Pelomedusa species, we identify two further clades from Cameroon and Sudan with similar levels of genetic divergence that remain unnamed candidate species. We also note that some problematical terrapins from South Africa and Somalia may represent two additional candidate species. Some of the Pelomedusa species are morphologically distinctive, whilst others can only be identified by molecular markers and are therefore morphologically cryptic taxa.
Full Text Available A common problem when trying to identify the sex of hatchling turtles is that juveniles are not obviously externally dimorphic and current techniques to identify sex are often invasive. In this paper, 300 eggs of Podocnemis expansa from Bananal Island, state of Tocantins (Brazil, were incubated at constant temperatures. The carapaces of the hatchlings were photographed and subjected to geometric morphometric analysis. The hatchlings were subsequently euthanized and had their gonads removed for sex determination. The pivotal temperature of P. expansa was 33.5ºC, confirming that this species has the highest pivotal temperature among reptiles. Geometric morphometric analysis of the shape of the carapace proved efficient in differentiating the sex of the hatchlings and confirmed that this methodology can be efficient for studies that need to ascertain the sex ratio in P. expansa hatchlings.
Ernst, Carl H.; Laemmerzahl, Arndt F.; Lovich, Jeffrey E.
The turtle Cuora amboinensis has an extensive distribution covering most of southern mainland Asia, Indonesia, and extending to the Philippine Islands. Unlike many species, C. amboinensis occurs on both sides of Wallace's Line separating Asian and Australian flora and fauna. Four subspecies are currently recognized; Cuora a. kamaroma (southern continental Asia, Java and the northern Philippines [introduced]), C. a. lineata (Kachin Province, Myanmar [Burma] and adjacent Yunnan Province, China), C. a. couro (Sumatra, Java, Sumbawa, and adjacent smaller Indonesian islands); and C. a. amboinensis (Moluccas, Sulawesi, Philippines). Five pattern and 33 morphological characters were examined for variation in 691 individuals from throughout the species' range. Our analyses suggest that only two presently recognized subspecies are valid: amboinensis andkamaroma. Neither couro nor lineata are supported by our analysis. We recommend that C. a. couroshould be synonymized with the species C. amboinensis and C. a. lineata with the subspecies C. a. kamaroma.
Ana Lucía BERMUDEZ-ROMERO
Full Text Available El objetivo del estudio fue identificar y explicar la influencia del hábitat sobre los nidos de la tortuga “cupiso” (Podocnemis sextuberculata, en el lago Erepecu ubicado en la Reserva Biológica del Río Trombetas (REBIO-Trombetas; Pará-Brasil. Inicialmente se describieron las principales características de las playas escogidas, los nidos encontrados fueron marcados y cercados para su protección contra la depredación natural. A través de regresiones lineales simples se determinó que el número de nidos por playa se correlacionó significativamente con el “área” de las playas. Los resultados muestran que la selección del lugar de nidificación en las playas del lago Erepecu por P. sextuberculata, podría depender en unos escenarios de compensación entre las amenazas naturales y un hábitat adecuado para anidar. Debido a la gran fluctuación hidrológica anual, es posible que el factor determinante para selección de las hembras sea el riesgo de exposición de las hembras durante la búsqueda de área de anidación, más que el tipo de hábitat en particular.
Duncan, W P; Marcon, J L
The metabolic profiles of selected tissues were analyzed in hatchlings of the Amazonian freshwater turtles Podocnemis expansa, P. unifilis and P. sextuberculata. Metabolic design in these species was judged based on the key enzymes of energy metabolism, with special emphasis on carbohydrate, lipid, amino acid and ketone body metabolism. All species showed a high glycolytic potential in all sampled tissues. Based on low levels of hexokinase, glycogen may be an important fuel for these species. The high lactate dehydrogenase activity in the liver may play a significant role in carbohydrate catabolism, possibly during diving. Oxidative metabolism in P. sextuberculata appears to be designed for the use of lipids, amino acids and ketone bodies. The maximal activities of 3-hydroxyacyl-CoA dehydrogenase, malate dehydrogenase, glutamine dehydrogenase, alanine aminotransferase and succinyl-CoA keto transferase display high aerobic potential, especially in muscle and liver tissues of this species. Although amino acids and ketone bodies may be important fuels for oxidative metabolism, carbohydrates and lipids are the major fuels used by P. expansa and P. unifilis. Our results are consistent with the food habits and lifestyle of Amazonian freshwater turtles. The metabolic design, based on enzyme activities, suggests that hatchlings of P. unifilis and P. expansa are predominately herbivorous, whereas P. sextuberculata rely on a mixed diet of animal matter and vegetation.
Lucélia G. Vieira
Full Text Available Abstract: With aim to report the ontogeny of the osseous elements of the carapace in Peurodiras, 62 embryos and 43 nestlings of Podocnemis expansa were collected and submitted to the clearing and staining technique of bones and cartilages and study of serial histological slices. The carapace has mixed osseous structure of endo and exoskeleton, formed by 8 pairs of costal bones associated with ribs, 7 neural bones associated with neural arches, 11 pairs of peripheral bones, 1 nuchal, 1 pygal and 1 suprapygal. This structure begins its formation in the beginning of stage 16 with the ossification of the periosteal collar of the ribs. With exception of the peripheral bones, the other ones begin their ossification during the embrionary period. In histologic investigation it was found that the costal bones and neural bones have a close relation to the endoskeleton components, originating themselves as intramembranous expansions of the periosteal collar of the ribs and neural arches, respectively. The condensation of the mesenchyme adjacent to the periosteal collar induces the formation of spikes that grow in trabeculae permeated by fibroblasts below the dermis. The nuchal bone also ossifies in an intramembranous way, but does not show direct relation to the endoskeleton. Such information confirms those related to the other Pleurodira, mainly with Podocnemis unifilis, sometimes with conspicuous variations in the chronology of the ossification events. The formation of dermal plates in the carapace of Pleurodira and Criptodira follow the same pattern.
Alhaboubi, Amer Rasool; Pollard, Dana A; Holman, Patricia J
A severely underweight alligator snapping turtle Macrochelys temminckii Troost in Harlan, 1835, was found near Tyler, Texas, and taken to the Caldwell Zoo. Blood films were submitted to Texas A&M University, College Station, Texas, for morphological and molecular identification of haemogregarine-like inclusions in the red blood cells. Intraerythrocytic Haemogregarina sp. forms were found on microscopic examination at a parasitemia of <1 %. The morphology and morphometric data for the forms indicate similarity to Haemogregarina macrochelysi n. sp. Telford et al., 2009, previously reported in alligator snapping turtles in Florida and Georgia, but two characteristic stage forms were not shared between H. macrochelysi n. sp. and the parasite found in this report. The haemogregarine 18S ribosomal RNA gene (1555-bp fragment) was amplified and cloned, and five clones sequenced. The sequences were deposited in the NCBI GenBank database. All five showed ∼96 % identity to Haemogregarina balli Paterson and Desser, 1976, Hepatozoon sp., and Hemolivia stellata Petit et al., 1990. A 774-bp segment shared 98-99 % identity with the corresponding Haemogregarina sp. rDNA sequence (KR006985) from Caspian turtles (Mauremys caspica McDowell, 1964) in Iran. A neighbor-joining phylogenetic tree generated from aligned sequences from the clones, 26 hematozoa, Adelina dimidiata Schneider, 1875, and Cryptosporidium serpentis Levine, 1980, revealed the cloned sequences clustered on their own branch within the Haemogregarina spp. clade. No genetic data are available for H. macrochelysi n. sp. at this time, so it remains unclear if this parasite in a Texas alligator snapping turtle is conspecific with H. macrochelysi n. sp.
Ennen, J.R.; Kreiser, B.R.; Qualls, C.P.; Lovich, J.E.
The turtle genus Graptemys consists of 15 recognized taxa, distinguished largely on the basis of pigmentation pattern (i.e., soft tissue and shell), head size, and shell morphology. However, phylogenetic studies have shown limited sequence divergence within the genus and between Graptemys oculifera and Graptemys flavimaculata relative to most other members of the Emydidae. Graptemys oculifera of the Pearl River drainage and G. flavimaculata of the Pascagoula River drainage have been recognized as species since 1890 and 1954, respectively. However, the description of G. flavimaculata was based on a limited number of morphological characters. Several of these characters overlap between G. flavimaculata and G. oculifera, and no attempt was made to test for significant morphological differentiation. In this study, we reevaluated the morphological and genetic distinctiveness of G. flavimaculata and G. oculifera with (1) multivariate statistical analyses of 44 morphological characters and (2) 1,560 bp of sequence data from two mitochondrial genes (control region and ND4). The morphological and molecular analyses produced incongruent results. The principal components analysis ordinations separated the two species along a pigmentation gradient with G. flavimaculata having more yellow pigmentation than G. oculifera. Likewise, clustering analyses separated the specimens into two distinct groups with little overlap between the species. Our mitochondrial data supported previous findings of limited genetic differentiation between the two species. However, the results of our morphological analyses, in conjunction with recently published nuclear gene sequence data, support the continued recognition of the two species. Copyright 2010 Society for the Study of Amphibians and Reptiles.
Merchán, Manuel; Coll, Marta; Fournier, Raúl
The African Spur tortoise, Geochelone sulcata, has been introduced to Costa Rica. A total of 31 tortoises were measured for 26 gross morphometry parameters. All individuals measured were inmature, aged from 5 to 34 months, and were born in captivity in La Garita de Alajuela, Costa Rica. Mean straight carapace length was 83.1 mm, mean straight plastron length was 68.3 mm and mean maximum height was 46.2 mm. All the measurements were correlated, except tail length and cloacal distance. Weight h...
Merchán, Manuel; Coll, Marta; Fournier, Raúl
The African Spur tortoise, Geochelone sulcata, has been introduced to Costa Rica. A total of 31 tortoises weremeasured for 26 gross morphometry parameters. All individuals measured were inmature, aged from 5 to 34months, and were born in captivity in La Garita de Alajuela, Costa Rica. Mean straight carapace length was 83.1mm, mean straight plastron length was 68.3 mm and mean maximum height was 46.2 mm. All the measurementswere correlated, except tail length and cloacal distance. Weight had t...
Merchán, Manuel; Coll, Marta; Fournier, Raúl
The African Spur tortoise, Geochelone sulcata, has been introduced to Costa Rica. A total of 31 tortoises were measured for 26 gross morphometry parameters. All individuals measured were inmature, aged from 5 to 34 months, and were born in captivity in La Garita de Alajuela, Costa Rica. Mean straight carapace length was 83.1 mm, mean straight plastron length was 68.3 mm and mean maximum height was 46.2 mm. All the measurements were correlated, except tail length and cloacal distance. Weight had the highest positive allometry coefficient. All the variables were joined in two Principal Components; tail length and cloacal distance in Factor 2 and the rest of them in Factor 1. Lack of correlation among tail measures and the other variables as well as their inclusion in a different Factor could be related with an incipient development of sexual dimorphism characters.
Full Text Available Previous studies have demonstrated that river-based surveys can provide an inexpensive source of information for neotropical zoologists, yet little information is available to inform the application of this technique for the long term monitoring of neotropical turtle species. We aimed to fill this gap by presenting an assessment of data collected during 333 river surveys over 50 months along rivers in a newly protected area in the Peruvian Amazon. A total of 14,138 basking Podocnemis unifilis Troschel, 1848 were recorded during 13,510 km of river-based surveys. We used generalized additive models (GAMs to explore the influence of a series of abiotic and seasonal variables on the recorded abundances at two temporal scales: monthly and per trip. Our analysis revealed that there was a significant increase in turtle abundances during the study period and we also found a significant seasonal periodicity in monthly abundances. Abiotic factors strongly influenced trip level abundances, with more individuals per kilometer recorded during sunny days in the dry season, with temperatures between 25 and 30°C. The results demonstrate that turtle populations are increasing following the establishment of the protected area and that river-based surveys are likely to be more effective when carried out within a limited set of key abiotic conditions.
Gabriel S. Ferreira
Full Text Available The extinct Stereogenyina turtles form a relatively diverse Podocnemididae lineage, with twelve described and phylogenetically positioned species. They are characterized by a wide geographic and temporal range, from the Eocene of Africa to the Pleistocene of Southeast Asia, and a peculiar palate morphology, with a secondary palate that is unique among side-necked turtles. Here, we describe a new Stereogenyina species, based on an almost complete skull from the middle Miocene Capadare Formation, of Venezuela. A new phylogenetic analysis supports the assignment of the new species to the genus Bairdemys. Based on geometric morphometrics analyses, we related the development of the stereogenyin secondary palate with the acquisition of a durophagous diet. Based on a review of the sedimentary environments where their fossils are found, we also propose that stereogenyins were a marine radiation of podocnemidid turtles, as corroborated by previous studies of fossil eggs and limb morphology. These two inferences allowed us to hypothesize that stereogenyins occupied an ecological niche similar to that of the extant Carettini sea turtles, and that the rise of the latter group may be related to the Stereogenyina diversity fall in the end of the Miocene.
Full Text Available Many Amazonian animals are a valuable food source, for the rural and urban people of the Peruvian forest. Their meat is called carne de monte, and is the third source of protein food in the region. Podocnemis unifilis (Yellow-spotted Amazon River turtle or taricaya is utilized for both its meat and eggs. The efforts for restocking this reptile through breeding in semicaptivity and captivity, has allowed increasing the population, and now they are abundant. However, the breeding process had not taken into account the sanitary aspect, and this information is barely known in Peru. With the objective of identifying the parasitic fauna of the taricaya, the digestive tracts of 12 females (2 adults, 10 young captured in the Pacaya Samiria National Reserve where examined. Different grades of parasitic infection were found in all turtles investigated. The parasites identified were the nematodes Serpinema amazonicus (100% and Ancyracanthus pinnatifidus Diesing, 1934 (83%, and the trematode Nematophila grandis (Diesing, 1939, Travassos, 1934 (100%. Ancyracanthus pinnatifidus is a new record from Peru.
Full Text Available Coelioscopy has been proven to be a valuable technique to determine the sex of juvenile chelonians. However, there is a disagreement regarding the proper way to identify testes and ovaries, which is a direct consequence of the lack of studies correlating the results of endoscopic examination with the histology of the gonad. In this blinded study we assessed two methods of sex determination in juvenile pond sliders (Trachemys scripta using endoscopy: via coelioscopy, with visualization of the gonads, and via cloacoscopy, with visualization of phallus/clitoris; we then compared the results of these procedures with the histology of the gonad. The results of gonad histology correlated 100% with the results from coelioscopic examination, but only 57.77% of the results obtained by cloacoscopy were accurate. Using cloacoscopy, 83.33% of males and 38.46% of females were misdiagnosed. Sex determination of juvenile pond sliders was considered accurate when coelioscopy was used, but inaccurate when cloacoscopy with identification of phallus/clitoris was attempted.
Hernández-Guzmán, Javier; Indy, Jeane Rimber; Yasui, George Shigueki; Arias-Rodriguez, Lenin
Mexico is a biodiverse country in several taxa as reptiles, that include several species of freshwater and marine turtles. Eventhough most of this group species are under protection, Tabasco State has nine native freshwater turtles, like Kinosternon leucostomum, Trachemys scripta and Staurotypus triporcatus that are very important in traditional dishes. This has resulted in a critical level of their populations, together with little biological knowledge for their conservation. Therefore, this study was dedicated to turtle cytogenetics. The study was conducted using the conventional methods for cytogenetics. The results showed the modal diploid and haploid number for K. leucostomum of 2n = 56 (2n = 56+3 microchromosomes "B") and 1n = 28 chromosomes in mitosis and meiosis, respectively. In T. scripta 2n = 50 chromosomes (2n = 50+2 microchromosomes "B") and 1n = 25 chromosomes were also characterized. Whereas in S. triporcatus we only report the 2 = 54 chromosomes (2n = 54+2 microchromosomes "B"). The karyological formula for K. leucostomum was integrated by 12 metacentric-submetacentric chromosomes "msm"/"A"+22 subtelocentric-telocentric chromosomes "stt"/"B"+22 telocentric chromosomes "T"/"C" with fundamental number (FN) of 90 chromosome arms. While T. scripta karyotype was integrated by 32 "msm/"A"+10 "stt"/"B"+8"T/"C" chromosomes, with FN of 92 arms. S. triporcatus karyotype formula was built up by 20 chromosomes "msm"/"A"+34 chromosomes "T"/"C" with FN of 74. The variation in chromosome classification, the fundamental number and the presence of supernumerary microchromosomes "B" in the studied species, were evidence of a particular chromosome cytotypes in Tabasco. We considered that the presence of microchromosomes "B" probably has different origins, and they may be very important as a pattern for the formation or separation of new species. This study also showed the absence of heterologous chromosomes between the females and males karyotypes from the studied species.
Full Text Available This study provides an analysis of the occurrence and the spatial and temporal distribution of marine turtle strandings found in the south of the State of Bahia. Data was collected between January 2006 and June 2008. This study covers an area of 220 km of the southern coast of Bahia State (northeastern Brazil, and spatial analyses were made considering data collected in three bases suported by Petrobras-Petróleo Brasileiro S/A distributed in the area. The records were sorted according to month and year, species, age group and sex. A total of 260 stranding were reported: 183 of Chelonia mydas (74.1%, the most frequent species. The highest number of strandings was recorded in Gamboa do Morro Base. Juveniles presented the highest densities, but no differences between adults and small juveniles were detected. Males were more frequently stranded in Gamboa do Morro Base, while females were more frequent in Ilhéus Base. An increase in the number of stranding between 2006 and 2008 was noted; moreover, the months with more records were January, February, March, October and December. The number of stranding events was discontinuously distributed in the study area. This study also demonstrated the usefulness of implement different strategies of recording marine turtle strandings: direct monitoring efforts (patrol in remote beaches and educational campaigns applied on beaches frequented by tourists. This study demonstrated that, despite spatial nearby, the three bases attend independent biological systems and show different stranding dynamics, thus different conservancy actions should be implemented in order to improve the knowledge on natural history of sea-turtles in the southern coast of Bahia State.
de Carvalho, Robson Henrique; Lacerda, Pedro Dutra; da Silva Mendes, Sarah; Barbosa, Bruno Corrêa; Paschoalini, Mariana; Prezoto, Fabio; de Sousa, Bernadete Maria
Assessment of marine debris ingestion by sea turtles is important, especially to ensure their survival. From January to December 2011, 23 specimens of five species of sea turtles were found dead or dying after being rehabilitated, along the coast of the municipality of Rio de Janeiro, Brazil. To detect the presence of marine debris in the digestive tract of these turtles, we conducted a postmortem examination from the esophagus until the distal portion of the large intestine for each specimen. Of the total number of turtles, 39% had ingested marine debris such as soft plastic, hard plastic, metal, polyethylene terephthalate (PET) bottle caps, human hair, tampons, and latex condoms. Five of the seven sea turtles species are found along the Brazilian coast, where they feed and breed. A large number of animals are exposed to various kinds of threats, including debris ingestion.
Full Text Available We quantified total mercury concentrations in eggshells, egg yolks, and embryos from 16 nests of the Colombian Slider (Trachemys callirostris. Nests were collected in different stages of development, but estimated time of incubation in natural substrates was not correlated with mercury levels in the eggs, suggesting that mercury was not absorbed from the substrate, but more likely passed on to the embryos during folliculogenesis by the reproductive females who had bioaccumulated the mercury from environmental sources. Mean mercury concentrations were higher in embryos than in eggshells or egg yolks, indicating that embryos also bioaccumulate mercury present in other egg tissues. Intra-clutch variation in egg yolk mercury concentrations was relatively high. Egg yolk mercury concentrations were not associated with any of the fitness proxies we quantified for the nests (hatching success rates, initial neonate sizes and first-month juvenile growth rates. After five months of captive rearing in a mercury-free laboratory environment, most juveniles had eliminated the mercury from their tissues.
Amanda Maria Picelli
Full Text Available Seventy-five turtles Podocnemis expansa in the Brazilian Amazon were examined for the presence of ectoparasites and hemoparasites. Samplings were performed in three study areas in the state of Tocantins, Brazil. Twenty-five specimens were sampled per study area (a commercial breeding facility, an indigenous subsistence breeding facility and a wild population of the Javaés River. Hemoparasites of the genus Haemogregarina were found in 66% (50/75 of the turtle specimens, and the infections were restricted to the commercial breeding facility and to the wild population of the Javaés River. The mean level of parasitemia was 54/2,000 erythrocytes (2%. There was no correlation between the body condition index of the chelonians and the level of parasitemia, with no significant difference between genders. No leeches were observed during the physical exams in any of the study areas, but the specimens from the commercial breeding facility were in poor physical condition with shell deformities and the presence of a relatively high amount of skin ulcerations, most likely caused by fungi and bacteria. This was the first study to record the occurrence of hemogregarines on a population scale in P. expansa and helps to increase knowledge about hemoparasites in chelonians in Brazil.
A Cordero, Gerardo; Janzen, Fredric J
Normal developmental staging tables often undergo expansion and enhancement in response to advancing research paradigms and technologies. The Painted Turtle, Chrysemys picta, has long been a preferred reference taxon for comparative embryology and recently became the first turtle species to feature a sequenced genome. However, modern descriptive studies on embryogenesis are lacking and an earlier developmental staging table has been ignored. To address these problems, we re-evaluated descriptions of developmental stages by studying embryos under standardized laboratory conditions. We created an enhanced normal developmental staging table that clarifies and validates previous descriptions of developmental processes in this species. Moreover, we emphasized description of turtle-specific developmental characters such as the carapacial ridge. We demonstrated that embryo growth rate, length of incubation period, and timing to developmental stages are predictable under controlled environmental conditions. The appearance of characters associated with eye, limb, and shell anatomy was congruent with observations made in other turtle species. To reduce experimental bias, we recommend the use of our enhanced staging table when describing embryogenesis in the Painted Turtle. Copyright © 2013 Wiley Periodicals, Inc.
Julia Azanza Ricardo
Full Text Available The nesting colony of green sea turtles Chelonia mydas at Guanahacabibes Peninsula Biosphere Reserve and National Park is one of the largest in the Cuban archipelago; however, little information about its nesting ecology is available. Temporal and spatial variation in nesting and reproductive success as well as morphometric characteristics of gravid females were used to ecologically characterize this colony. Nine beaches of the Southernmost coast of Guanahacabibes Peninsula were monitored for 14 years 1998-2012 to determine green turtle nesting activity, from May to September peak nesting season in this area. Beach dimensions were measured to determine nest density using the length and the area. Afterward the beaches were divided in two categories, index and secondary. Females were measured and tagged to compare new tagged females 823 with returning tagged females 140. Remigration interval was also determined. Temporal variation was identified as the annual number of nesting emergences and oviposits per female, with apparent peaks in reproductive activity on a biennial cycle in the first six years followed by periods of annual increase in nest number 2003-2008 and periods of decreasing number of nests 2010-2012. We also found intra-seasonal variation with the highest nesting activity in July, particularly in the second half of the month. The peak emergence time was 22:00-02:00hr. In terms of spatial variation, smaller beaches had the highest nest density and nesting was more frequent 6-9m from the high tide line, where hatchling production was maximized although hatchling success was high on average, above 80. Morphometric analysis of females was made and newly tagged turtles were smaller on average than remigrants. Our results are only a first attempt at characterizing Guanahacabibes populations but have great value for establishing conservation priorities within the context of national management plans, and for efficient monitoring and protection of nesting beaches.
Rovatsos, Michail; Praschag, Peter; Fritz, Uwe; Kratochvšl, Lukáš
Turtles demonstrate variability in sex determination ranging from environmental sex determination (ESD) to highly differentiated sex chromosomes. However, the evolutionary dynamics of sex determining systems in this group is not well known. Differentiated ZZ/ZW sex chromosomes were identified in two species of the softshell turtles (Trionychidae) from the subfamily Trionychinae and Z-specific genes were identified in a single species. We tested Z-specificity of a subset of these genes by quantitative PCR comparing copy gene numbers in male and female genomes in 10 species covering the phylogenetic diversity of trionychids. We demonstrated that differentiated ZZ/ZW sex chromosomes are conserved across the whole family and that they were already present in the common ancestor of the extant trionychids. As the sister lineage, Carettochelys insculpta, possess ESD, we can date the origin of the sex chromosomes in trionychids between 200 Mya (split of Trionychidae and Carettochelyidae) and 120 Mya (basal splitting of the recent trionychids). The results support the evolutionary stability of differentiated sex chromosomes in some lineages of ectothermic vertebrates. Moreover, our approach determining sex-linkage of protein coding genes can be used as a reliable technique of molecular sexing across trionychids useful for effective breeding strategy in conservation projects of endangered species. PMID:28186115
Ernst, Carl H.; Lovich, Jeffrey E.
The taxonomy of Asian box turtles of the genus Cuora is complicated by the description of numerous valid and invalid taxa over the last several decades. However, some characteristics used to differentiate species are questionable. Members of the C. flavomarginata complex are defined by some, but not all, taxonomists as having reduced interanal seam lengths relative to other species. We examined the ratio of interanal scute seam length divided by midline anal scute length in C. flavomarginata and C. evelynae. Hatchlings show a seam that divides 100% of the anal scute along the midline. As individuals increase in carapace length, there is a tendency for the percentage to decrease, especially in females, although there is considerable overlap. We suggest that the decrease in interanal seam length is due to abrasion of the plastron on the substrate as turtles grow larger and older. Differences in habitat substrates across the range of the species may contribute to the wide variation we observed.
Full Text Available We obtained 8 digestive tract of the turtle Podocnemis expansa and 18 of P. unifilis from the Belen market (Iquitos, Peru. Only digeneans were studied and identified. Four species were found: Nematophila grandis (Diesing, 1839 Travassos, 1934, Halltrema avitellina Lent & Freitas, 1939, Podocnemitrema papillosum Alho & Vicente, 1964 and Telorchis hagmanni Lent & Freitas, 1937. Halltrema avitellina, Podocnemitrema papillosum and Telorchis hagmanni are new records from Peru.
Azanza Ricardo, Julia; Ibarra Martín, Maria E; González Sansón, Gaspar; Abreu Grobois, F Alberto; Eckert, Karen L; Espinosa López, Georgina; Oyama, Ken
The nesting colony of green sea turtles (Chelonia mydas) at Guanahacabibes Peninsula Biosphere Reserve and National Park is one of the largest in the Cuban archipelago; however, little information about its nesting ecology is available. Temporal and spatial variation in nesting and reproductive success as well as morphometric characteristics of gravid females were used to ecologically characterize this colony. Nine beaches of the Southernmost coast of Guanahacabibes Peninsula were monitored for 14 years (1998-2012) to determine green turtle nesting activity, from May to September (peak nesting season in this area). Beach dimensions were measured to determine nest density using the length and the area. Afterward the beaches were divided in two categories, index and secondary. Females were measured and tagged to compare new tagged females (823) with returning tagged females (140). Remigration interval was also determined. Temporal variation was identified as the annual number of nesting emergences and oviposits per female, with apparent peaks in reproductive activity on a biennial cycle in the first six years followed by periods of annual increase in nest number (2003-2008) and periods of decreasing number of nests (2010-2012). We also found intra-seasonal variation with the highest nesting activity in July, particularly in the second half of the month. The peak emergence time was 22:00-02:00 hr. In terms of spatial variation, smaller beaches had the highest nest density and nesting was more frequent 6-9m from the high tide line, where hatchling production was maximized although hatchling success was high on average, above 80%. Morphometric analysis of females was made and newly tagged turtles were smaller on average than remigrants. Our results are only a first attempt at characterizing Guanahacabibes' populations but have great value for establishing conservation priorities within the context of national management plans, and for efficient monitoring and protection of nesting beaches.
da Silva Mendes, Sarah; de Carvalho, Robson Henrique; de Faria, Adriana Fonseca; de Sousa, Bernadete Maria
Chelonia mydas is distributed in several regions of the world and they are common in coastal regions and around islands. Between August 2008 and July 2009, 20 specimens of C. mydas were found dead on the beaches of Ubatuba, São Paulo, Brazil. The stomachs were removed and anthropogenic wastes were separated according their malleability and color. Of those animals, nine had ingested marine debris. Soft plastic was the most frequent among the samples and the majority of fragments was white or colorless and was between zero and five cm. Many studies have shown a high incidence of eating waste for some species of sea turtles. The record of ingestion of mostly transparent and white anthropogenic wastes in this study strengthens the hypothesis that these animals mistake them for jellyfish. Although the intake of anthropogenic waste causes impact on the lives of sea turtles, such studies are still scarce in Brazil.
Werneck, M R; Silva, R J
The present study offers a parasitological analysis of juvenile individuals of the green turtle ( Chelonia mydas ) found on the Brazilian coast between 2004 and 2011. Helminths were found in 90 out of 136 individuals (66.2%, CI = 57.7-74.0). In total, 29,411 helminths were collected, belonging to the families Brachycoeliidae, Cladorchiidae, Microscaphidiidae, Pronocephalidae, Rhytidodidae, and Spirorchiidae. Mean species richness was 4.74 (CI = 4.03-5.46), the mean intensity was 327 (CI = 223-489), and the mean abundance was 216 (CI = 146-339). This study also reports new geographical records for: Angiodictyum longum, Angiodictyum parallelum, Rameshwarotrema uterocrescens, Pyelosomum cochlear, Schizamphistomum scleroporum, Cymatocarpus solearis, and Neospirorchis sp. This is the first analysis of helminth composition in juveniles of green turtles.
Vera, Vicente; Buitrago, Joaquín
The second major nesting-site for green turtles in the Caribbean is Isla de Aves, an island protected as a wildlife refuge since 1972, located at 650km Northeast from La Guaira, Venezuela. In this island, the nesting population monitoring started in 1972 and in a more continuous way after 1978, when a Scientific-Naval Station was established and scientific observations started. Since historical data show that female captures had severely affected population levels in this island before 1978, this study aim to describe recent reproductive activities. For this, during the nesting seasons of 2001-2002 and 2005-2008, nesting females were measured and tagged using metal flipper tags. A total of 458 nights were sampled observing 5 154 female emergences, with a maximum of 53 in a single night. Non-observed emergences were calculated fitting the temporal distribution of observed emergences to a normal curve. Total emergences estimated varied from X=637.1+/-106.6 in 2001 to X =2 853+/-42.5 in 2008 (ANOVA F(6.5df)=60.37, p<0.0001). Internesting interval in the same season was estimated in X=10.71+/-1.32 days. Clutch frequency in a nesting season was calculated as X=1.71+/-1.6 times per female and season. Estimated number of nesting females per year varied from X=373+/-12.5 females in 2001 to X=l 669+/-56.1 females in 2008 (ANOVA F 55.6df)=89.42, p<0.0001); with a positive and significant trend (r=0.842, p=0.036). Results show that nesting females numbers are increasing. We suggest that the protection of the nesting area for more than 30 years, has contributed with this population increase.
Full Text Available Cuantificamos la concentración total de mercurio en cáscaras, yemas y embriones de 16 nidos de hicotea (Trachemys callirostris. Los nidos fueron colectados en diferentes estadios de desarrollo embrionario. No hubo una correlación entre el tiempo estimado desde el desove y los niveles de mercurio en los huevos, sugiriendo que el metal no fue absorbido del substrato, sino que probablemente éste fue transferido a los huevos durante el proceso de foliculogénesis en las hembras reproductivas, las cuales bioacumularon el mercurio de fuentes ambientales. La concentración promedio de mercurio fue mayor en los embriones que en las cáscaras o yemas, indicando que los embriones también bioacumulan el metal presente en otros tejidos del huevo. La variación de la concentración de mercurio dentro de una misma nidada fue relativamente alta. Las concentraciones de mercurio en las yemas no estuvieron asociadas con ninguna de las medidas de fitness que fueron evaluadas (éxito de eclosión, tamaño inicial de los neonatos y tasas de crecimiento de los juveniles en el primer mes. Después de cinco meses de mantenimiento en cautiverio, en un ambiente libre de mercurio, 86 % de los juveniles había eliminado completamente este metal de sus tejidos.
Full Text Available The metabolic profiles of selected tissues were analyzed in hatchlings of the Amazonian freshwater turtles Podocnemis expansa, P. unifilis and P. sextuberculata. Metabolic design in these species was judged based on the key enzymes of energy metabolism, with special emphasis on carbohydrate, lipid, amino acid and ketone body metabolism. All species showed a high glycolytic potential in all sampled tissues. Based on low levels of hexokinase, glycogen may be an important fuel for these species. The high lactate dehydrogenase activity in the liver may play a significant role in carbohydrate catabolism, possibly during diving. Oxidative metabolism in P. sextuberculata appears to be designed for the use of lipids, amino acids and ketone bodies. The maximal activities of 3-hydroxyacyl-CoA dehydrogenase, malate dehydrogenase, glutamine dehydrogenase, alanine aminotransferase and succinyl-CoA keto transferase display high aerobic potential, especially in muscle and liver tissues of this species. Although amino acids and ketone bodies may be important fuels for oxidative metabolism, carbohydrates and lipids are the major fuels used by P. expansa and P. unifilis. Our results are consistent with the food habits and lifestyle of Amazonian freshwater turtles. The metabolic design, based on enzyme activities, suggests that hatchlings of P. unifilis and P. expansa are predominately herbivorous, whereas P. sextuberculata rely on a mixed diet of animal matter and vegetation.
Marc E H Jones
Full Text Available BACKGROUND: Sea turtles (Chelonoidea are a charismatic group of marine reptiles that occupy a range of important ecological roles. However, the diversity and evolution of their feeding anatomy remain incompletely known. METHODOLOGY/PRINCIPAL FINDINGS: Using computed tomography and classical comparative anatomy we describe the cranial anatomy in two sea turtles, the loggerhead (Caretta caretta and Kemp's ridley (Lepidochelys kempii, for a better understanding of sea turtle functional anatomy and morphological variation. In both taxa the temporal region of the skull is enclosed by bone and the jaw joint structure and muscle arrangement indicate that palinal jaw movement is possible. The tongue is relatively small, and the hyoid apparatus is not as conspicuous as in some freshwater aquatic turtles. We find several similarities between the muscles of C. caretta and L. kempii, but comparison with other turtles suggests only one of these characters may be derived: connection of the m. adductor mandibulae internus into the Pars intramandibularis via the Zwischensehne. The large fleshy origin of the m. adductor mandibulae externus Pars superficialis from the jugal seems to be a characteristic feature of sea turtles. CONCLUSIONS/SIGNIFICANCE: In C. caretta and L. kempii the ability to suction feed does not seem to be as well developed as that found in some freshwater aquatic turtles. Instead both have skulls suited to forceful biting. This is consistent with the observation that both taxa tend to feed on relatively slow moving but sometimes armoured prey. The broad fleshy origin of the m. adductor mandibulae externus Pars superficialis may be linked to thecheek region being almost fully enclosed in bone but the relationship is complex.
Joyce, Walter G; Rabi, Márton; Clark, James M; Xu, Xing
Turtles (Testudinata) are a successful lineage of vertebrates with about 350 extant species that inhabit all major oceans and landmasses with tropical to temperate climates. The rich fossil record of turtles documents the adaptation of various sub-lineages to a broad range of habitat preferences, but a synthetic biogeographic model is still lacking for the group. We herein describe a new species of fossil turtle from the Late Jurassic of Xinjiang, China, Sichuanchelys palatodentata sp. nov., that is highly unusual by plesiomorphically exhibiting palatal teeth. Phylogenetic analysis places the Late Jurassic Sichuanchelys palatodentata in a clade with the Late Cretaceous Mongolochelys efremovi outside crown group Testudines thereby establishing the prolonged presence of a previously unrecognized clade of turtles in Asia, herein named Sichuanchelyidae. In contrast to previous hypotheses, M. efremovi and Kallokibotion bajazidi are not found within Meiolaniformes, a clade that is here reinterpreted as being restricted to Gondwana. A revision of the global distribution of fossil and recent turtle reveals that the three primary lineages of derived, aquatic turtles, including the crown, Paracryptodira, Pan-Pleurodira, and Pan-Cryptodira can be traced back to the Middle Jurassic of Euramerica, Gondwana, and Asia, respectively, which resulted from the primary break up of Pangaea at that time. The two primary lineages of Pleurodira, Pan-Pelomedusoides and Pan-Chelidae, can similarly be traced back to the Cretaceous of northern and southern Gondwana, respectively, which were separated from one another by a large desert zone during that time. The primary divergence of crown turtles was therefore driven by vicariance to the primary freshwater aquatic habitat of these lineages. The temporally persistent lineages of basal turtles, Helochelydridae, Meiolaniformes, Sichuanchelyidae, can similarly be traced back to the Late Mesozoic of Euramerica, southern Gondwana, and Asia. Given
Ex situ Annual Egg—Laying Cycles of Rhinoclemmys melanosterna, R. diademata and their Hybrids (Reptilia: Testudines: Emydidae: Batagurinae Ciclos de postura anual ex situ de Rhinoclemmys melanosterna, R. diademata y de sus hibridos (Reptilia: Testudines: Emydidae: Batagurinae
Full Text Available R. melanosterna, R. diademata and their hybrids (R. melanosterna x R. diademata present annual continuous egg-laying cycles with maximum frequency during the rainy season. The period of maximal annual egg-laying for hybrid R. melanosterna x R. diademata is earlier than its parents. Aparently there is not direct relation between the ad libitum food offer and Rhinoclemmys sp egg-laying cycles.Registros históricos de postura ex situ de huevos de tortugas del género Rhinoclemmys en la Estación de Biología Tropical Roberto Franco (EBTRF, correspondientes a los años 1991-1999, fueron analizados en relación con factores climáticos locales. Rhinoclemmys melanosterna, Rhinoclemmys diademata y sus híbridos presentan ciclos de postura continuos durante el año con máxima frecuencia durante la estación lluviosa. La época de postura máxima durante un ciclo anual del híbrido R. melanosterna x R. diademata es más temprana que la de sus progenitores de origen. No parece existir relación directa entre la oferta de alimento ad libitum y los ciclos de postura de Rhinoclemmys sp.
Hematological profile of Chelonia mydas (Testudines, Cheloniidae according to the severity of fibropapillomatosis or its absence Perfil hematológico de Chelonia mydas (Testudines, Cheloniidae de acordo com o grau de acometimento pela fibropapilomatose e sua ausência
Full Text Available The green turtle Chelonia mydas feeds and nests in the Brazilian coastal area and is considered an endangered species by the World Conservation Union (IUCN 2009 and threatened by the Red List of Brazilian Fauna (Ministério do Meio Ambiente 2009. Fibropapillomatosis is a disease characterized by benign skin tumors (fibropapillomas, and it is one of the main threats to the survival of this species. Studies suggest the involvement of viruses as infectious agents associated with environmental and genetic factors. Blood samples were collected from 45 turtles captured in the coastal area of the state of Sao Paulo, Brazil. From these, 27 were affected by fibropapillomas and 18 were tumor free. Biometrical data on the turtles, size, location and quantity of tumors were recorded. The area occupied by fibropapillomas per animal was calculated and four groups were determined according to severity of the disease or its absence. The objective of the study was to compare hemogram results of the sea turtles classified in these four groups. The lowest hematocrit value was observed in severely affected animals. In the hemoglobin assay, the highest value was observed in the group of tumor free turtles and the lowest, in animals severely affected. Lymphocyte counts and curved carapace length were on the verge of statistical significance.Chelonia mydas, denominada tartaruga verde, é uma tartaruga marinha que frequenta o litoral brasileiro para alimentação e nidificação e é considerada em perigo de extinção pela IUCN (World Conservation Union, 2009 e ameaçada pela Lista Vermelha da Fauna Brasileira (Ministério do Meio Ambiente, 2009. A fibropapilomatose, doença caracterizada por tumores cutâneos benignos (fibropapilomas, é uma das mais importantes ameaças à sobrevivência dessa espécie. Pesquisas sugerem o envolvimento de agentes infecciosos virais em associação com fatores ambientais e genéticos. Foram colhidas amostras sanguíneas de 45 tartarugas provenientes do litoral do estado de São Paulo, Brasil, sendo 18 sem fibropapilomas e 27 acometidas. Dados de biometria das tartarugas, quantidade, localização e tamanho dos tumores foram anotados. Foi realizado o cálculo da área de fibropapilomas por animal e foram estipulados 4 grupos de acordo com o grau de acometimento e sua ausência. O objetivo foi realizar uma comparação entre os hemogramas das tartarugas marinhas classificadas nos 4 grupos. Animais de grau grave apresentaram o menor valor para hematócrito. Para dosagem de hemoglobina, observou-se que o maior valor foi para o grupo de tartarugas sem fibropapilomas e o menor para o de grau grave. Os valores de linfócitos e comprimento curvilíneo da carapaça beiraram a significância estatística.
Avaliação de padrão irregular dos escudos do casco em Podocnemis expansa e Podocnemis unifilis (Testudines, Podocnemididae Evaluation of irregular pattern of the shell scutes in Podocnemis expansa and Podocnemis unifilis (Testudines, Podocnemididae
Full Text Available O casco de Podocnemis expansa (tartaruga-da-amazônia e Podocnemis unifilis (tracajá, os maiores quelônios de água doce da América do Sul, é uma estrutura única que diferencia esse grupo dos vertebrados atuais e está associado a alguns padrões comportamentais. O padrão dos escudos que compõem o casco dos quelônios é bastante uniforme, mas variações já foram descritas e analisadas para um grande número de espécies em todas as famílias existentes atualmente. Averiguou-se a incidência de irregularidades do padrão de escutelação no casco dessas espécies, durante os anos de 1999 a 2003 no rio Javaés, entorno do Parque Nacional do Araguaia e da Área de Proteção Ambiental Bananal/ Cantão, oeste do estado do Tocantins. P. expansa se apresenta como uma espécie mais suscetível à ocorrência de anormalidades no casco em relação a P. unifilis. Sendo que, dos 14.378 filhotes avaliados, (13,32 % apresentavam irregularidades no padrão de escutelação enquanto que dos 1.329 filhotes de P. unifilis apenas (4,44 %. Em P. expansa, os defeitos ocorreram quase que em sua totalidade na carapaça (98,64 % e em menor número (83,05 % em P. unifilis.The shell of Podocnemis expansa (Amazonian giant River turtle and Podocnemis unifilis (Yellow-spotted Amazon River Turtle, the largest South American turtles, is a unique structure that differentiates them from other known vertebrates; and it is associated with some of their behavioral patterns. Their scute pattern is quite uniform however variations have been described and analyzed for a great number of other species of existing families. In the Javaés River, from 1999 to 2003, we observed an occurrence of abnormalities in their scute pattern. This was in the vicinity of the Araguaia National Park and the Bananal/ Cantão Environment Protected Area, west of Tocantins state. Of the both species, P. expansa is more susceptible to shell abnormalities. Of 14.378 P. expansa hatchlings observed, 13.32 % presented scute pattern abnormalities, while of 1.329 P. unifilis hatchlings, only 4.44 %. The incidence of abnormalities occurred only in the carapace, namely, 98.64% in P. expansa, and 83.05% in P. unifilis.
Radiographic anatomy aspects and gastrointestinal transit time in Podocnemis unifilis troschel, 1848 (Testudines, Podocnemididae. = Aspectos anátomo-radiográficos e tempo de trânsito gastrintestinal em Podocnemis unifilis troschel, 1848 (Testudines, Podocnemididae
Fernando Moraes Machado Brito
Full Text Available The present study analyzed the radiographic anatomy and determined thegastrointestinal transit time of Podocnemis unifilis. We used ten animals belonging to LAPAS from the Federal University of Uberlândia, Uberlândia, Minas Gerais State, Brazil. The animals were orally fed with a barium sulphate suspension at 10 mL kg-1 mixed with mineral oil, at a ratio of 70% of barium sulphate for 30% of mineral oil. Afterwards, the animals underwent radiography in a dorsum ventriloquoal position, with the X-ray device adjusted at 72 Kv and 200 mA, in time intervals to follow the permanency of contrast in the organism. Five minutes after the contrast was supplied, the stomach was filled. After sixteen hours the contrast advanced to the smallintestine. In 48 hours, the whole small intestine and part of the colon were fulfilled. On the 9th day the stomach was empty and the contrast advanced to the colon. On the 11th day, the colon was totally fulfilled, and the contrast was close to cloaca. On the 18th day all contrast was eliminated by the animal. Total time for contrast elimination was, in average, 17.6 ± 2.4 days, with the minimum of 12 and maximum of 22 days, with temperature at 27ºC. The digestion of the food was slower in the duodenum, and faster in the colon-rectum, which presents lower indices of repletion.Avaliou-se aspectos anátomo-radiográficos bem como o tempo de trânsito gastrintestinal em Podocnemis unifilis. Foram utilizados 10 animais pertencentes ao Laboratório de Pesquisas em Animais Silvestres(LAPAS da Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil. Aos animais foi administrada, por via oral, uma suspensão de sulfato de bário 10 mL kg-1 misturada com óleo mineral na proporção de 70% de sulfato de bário para 30% de óleo. Posteriormente, os animais foram radiografados dorso-ventralmente, com o aparelho de raios-X regulado para 72 Kv e 200 mA, em intervalos de tempo pré-estabelecidos. Em média, cinco minutos após a administração do contraste, a porção proximal do estômago estava preenchida. Após 16 horas, o contraste progrediu para o intestino delgado. Com 48 horas, todo o intestino delgado e parte do intestinogrosso estavam preenchidos. No 9 dia o estômago apresentava-se vazio e o contraste progrediu para o cólon. No 11 dia, o contraste encontrava-se na região do reto e o duodeno encontrava-se vazio. No 14 dia, o intestino delgado já estava totalmente sem preenchimento. No 18 dia, todo o contraste foi eliminado. O tempo total de eliminação do contraste foi, em média, de 17,6 ± 2,4 dias, sendo o mínimo de 12 e o máximo de 22 dias em temperatura média de 27°C. A digestão do alimento foi mais lenta no duodeno e mais alta no cólon-reto, que alcançou menores índices de repleção.
Anatomia vascular das artérias renais natomia e gonadais de Podocnemis unifilis Schweigger, 1812 (Testudines, Pelomedusidae = Vascular anatomy of renal and gonadal arteries of Podocnemis unifilis Schweigger, 1812 (Testudines-Pelomedusidae
Líria Queiroz Luz Hirano
Full Text Available Este trabalho foi desenvolvido com o intuito de enriquecer o conhecimento sobre a morfologia vascular das artérias renais e gonadais de Podocnemis unifilis, facilitando o entendimento da fisiologia clínica e cirúrgica destes animais. Foram utilizados cincoexemplares machos de Podocnemis unifilis (tracajá, coletados segundo a Licença nº 066/2004- Ibama/RAM. A artéria carótida esquerda e a veia femoral direita foram canuladas e, pelas mesmas, foi introduzida solução fisiológica para lavagem do sistema vascular; em seguida,aplicou-se solução de Neoprene Látex “450” corada com pigmento específico (Globo S/A Tintas e Pigmentos. O material foi fixado em solução de Formol tamponado a 10% por um período mínimo de 96h. Uma abertura central e de formato quadrangular foi feita na metade caudal do plastrão e casco, de forma a expor os ramos da artéria aorta que irrigam os rins e as gônadas. Observou-se que as artérias renais se originam da face ventral da artéria aorta dorsal, em número de dois pares para cada rim e, em um único exemplar, elas originaram uma artéria renal direita e duas esquerdas. A artéria gonadal surgiu a partir da artéria renal, e apenas um par penetrou pela face dorsal de cada gônada.This work was developed with the aim of enriching knowledge on the vascular morphology of renal and gonadal arteries of Podocnemisunifilis, thus increasing the understanding of the clinical and surgical physiology of these animals. Five Podocnemis unifilis males were used, collected according to license no. 066/2004-Ibama/RAM.The left carotid artery and right femoral vein were cannulated, and a serum solution was introduced to remove obstructions from the vascular system. A solution of Neoprene Latex “450” dye was injected. The material was fixed in a solution of 10% formaldehyde for a period of 96 hours. Next, a square-shaped central opening was made in the caudal end of the plastron and bridge, exposing the branches of the aorta that irrigate the kidneys and gonads. It was observedthat the renal arteries were originated from the ventral face of the dorsal aorta artery, in a number of two pairs per kidney and, in a single case, they originated one right and two left renal arteries.The gonadal artery was originated from the renal artery, and only one pair penetrated the dorsal face of each gonad.
Pérez, Emir; Ruiz, Ariel; Espinosa, Georgina; Lee, Idania
Gonad maturity is usually evaluated through macroscopic analysis of the gonads. In sea turtles, the maturation stages are associated with body size, depending on the studied marine stock. Fishermen classify turtles bigger than 65.0 cm as sexually mature. If they have secondary sex characters they are recorded as breeding males. We compared body size with macroscopic and microscopic gonad characteristics in two Cuban turtles. Eighteen individuals of C. mydas and twenty of E. imbricata was obtained from the legal fishery stock of Jardines del Rey Archipelago (Cuba), from October 2005 and 2006. In males, breeding condition (maximum spermiogenesis) was checked by histological analysis of the testes. In females, sexual maturity was identified by the presence of vitellogenic follicles or ovarian corpora. Most males were immature (C. mydas: 79.0 cm; E. imbricata: 73.1+/-4.9 cm, n=3) and lacked secondary sex characters. Some E. imbricata without a developed penis were in spermatogenic stages II to IV (i.e. pubescent). Most females were immature (C. mydas: 79.6+/-7.7 cm, n=17; E. imbricata: 69.0+/-7.1 cm, n=16; i.e.prepubescent and pubescent. The prepubescent females had ovaries with previtellogenic follicles near 1.0 mm in a compact and yellowish stroma. The pubescent females had ovaries with previtellogenic follicles between 2.0 and 3.0 mm. The stroma was more loosened and irrigated than in prepubescent turtles. The finding of spermatogenic activity in pubescent males indicates asynchrony between testicular and penial development in E. imbricata. The current phenotypical approach used by fishermen is not enough to determine sexual maturation in these turtles. The minimal size tentatively should be changed to: C. mydas: above 93.0 cm and E. imbricata above 79.0 cm.
Kern, Maximilian M.; Guzy, Jacquelyn C.; Lovich, Jeffrey E.; Gibbons, J. Whitfield; Dorcas, Michael E.
Because resources are finite, female animals face trade-offs between the size and number of offspring they are able to produce during a single reproductive event. Optimal egg size (OES) theory predicts that any increase in resources allocated to reproduction should increase clutch size with minimal effects on egg size. Variations of OES predict that egg size should be optimized, although not necessarily constant across a population, because optimality is contingent on maternal phenotypes, such as body size and morphology, and recent environmental conditions. We examined the relationships among body size variables (pelvic aperture width, caudal gap height, and plastron length), clutch size, and egg width of diamondback terrapins from separate but proximate populations at Kiawah Island and Edisto Island, South Carolina. We found that terrapins do not meet some of the predictions of OES theory. Both populations exhibited greater variation in egg size among clutches than within, suggesting an absence of optimization except as it may relate to phenotype/habitat matching. We found that egg size appeared to be constrained by more than just pelvic aperture width in Kiawah terrapins but not in the Edisto population. Terrapins at Edisto appeared to exhibit osteokinesis in the caudal region of their shells, which may aid in the oviposition of large eggs.
McAllister, Chris T; Bursey, Charles R; Freed, Paul S
One hundred and seven reptiles (11 families, 32 species) from the Northern and Western Cape Provinces of South Africa were examined for helminths. Twenty-three (22%) individual reptiles were found to harbor at least 1 species of nematode; 3 (7%) reptiles harbored multiple infections of 2 nematode species. Eight species within 5 families of Nematoda were found in the reptiles surveyed including 1 atractid, 1 diaphanocephalid, 1 heterakid, 3 pharyngodonids, and 2 physalopterans. Ten new host records are reported. A summary of the nematode parasites identified from South African reptiles is provided.
Zazhigin, Vladimir S.
Background The present-day amphibian and reptile fauna of Western Siberia are the least diverse of the Palaearctic Realm, as a consequence of the unfavourable climatic conditions that predominate in this region. The origin and emergence of these herpetofaunal groups are poorly understood. Aside from the better-explored European Neogene localities yielding amphibian and reptile fossil remains, the Neogene herpetofauna of Western Asia is understudied. The few available data need critical reviews and new interpretations, taking into account the more recent records of the European herpetofauna. The comparison of this previous data with that of European fossil records would provide data on palaeobiogeographic affiliations of the region as well as on the origin and emergence of the present-day fauna of Western Siberia. An overview of the earliest occurrences of certain amphibian lineages is still needed. In addition, studies that address such knowledge gaps can be useful for molecular biologists in their calibration of molecular clocks. Methods and Results In this study, we considered critically reviewed available data from amphibian and reptile fauna from over 40 Western Siberian, Russian and Northeastern Kazakhstan localities, ranging from the Middle Miocene to Early Pleistocene. Herein, we provided new interpretations that arose from our assessment of the previously published and new data. More than 50 amphibians and reptile taxa were identified belonging to families Hynobiidae, Cryptobranchidae, Salamandridae, Palaeobatrachidae, Bombinatoridae, Pelobatidae, Hylidae, Bufonidae, Ranidae, Gekkonidae, Lacertidae, and Emydidae. Palaeobiogeographic analyses were performed for these groups and palaeoprecipitation values were estimated for 12 localities, using the bioclimatic analysis of herpetofaunal assemblages. Conclusion The Neogene assemblage of Western Siberia was found to be dominated by groups of European affinities, such as Palaeobatrachidae, Bombina, Hyla, Bufo bufo, and a small part of this assemblage included Eastern Palaearctic taxa (e.g. Salamandrella, Tylototriton, Bufotes viridis). For several taxa (e.g. Mioproteus, Hyla, Bombina, Rana temporaria), the Western Siberian occurrences represented their most eastern Eurasian records. The most diverse collection of fossil remains was found in the Middle Miocene. Less diversity has been registered towards the Early Pleistocene, potentially due to the progressive cooling of the climate in the Northern Hemisphere. The results of our study showed higher-amplitude changes of precipitation development in Western Siberia from the Early Miocene to the Pliocene, than previously assumed. PMID:28348925
Mario Santoro; Carlos Mario Orrego; Giovanna Hernández Gómez
Con el objetivo de determinar la flora normal aerobia, cloacal y nasal de la tortuga lora (Lepidochelys olivacea ), entre los meses de julio y agosto del 2002,se colectaron muestras bacteriológicas de 45 quelonios aparentemente sanos,durante el desove en Playa Nancite,Parque Nacional Santa Rosa, Costa Rica, a través del uso de hisopos estériles que se introdujeron en la cloaca y en uno de los conductos nasales. De las muestras recolectadas se obtuvieron e identificaron un total de 99 aislamie...
Vieira, Lucélia Gonçalves
CAPITULO 2: O cágado Podocnemis expansa está distribuído pela bacia amazônica e ocorre em quase todos os seus afluentes. O esqueleto dos vertebrados é composto de cartilagem e osso e representa o produto de células de três linhagens embrionárias distintas. O crânio é formado por células da crista neural. O conhecimento do critério biológico para a seqüência de formação óssea é de suma importância para o entendimento da ontogenia. Assim, o propósito do presente estudo foi est...
Full Text Available The wild diet of Testudo graeca ibera in Dobrogea, Romania is investigated by direct observation. A clear predominance (over 95% of plant matter is noticed, with 25 plant species consumed. Moreover the ingestion of animal matter (carrion as well as calcareous earth was observed.
Full Text Available Coprological examination of ten Pink-ringed tent turtles Kachuga tentoria circumdata, recently imported from India, and three Burmese black turtles Melanochelys trijuga edeniana, imported from Myanmar, revealed the presence of two new species of Eimeria. Oocysts of Eimeria kachua n. sp. from K. t. circumdata are broadly oval to subspherical, 15.3 (13-18 × 13.9 (12-16 μm, with polar granule and subspherical oocyst residuum. Sporocyst elongatelly oval to spindle-shaped, 8.7 (7.5-10 × 4.9 (4-6 μm, with a knoblike Stieda body, covered with fine membranous cupola-like structures. Thin walled oocysts of Eimeria patta n. sp. from M. t. edeniana, have an irregular shape, influenced by the position of sporocysts, frequently with lobular irregularities, 12.6 (11-16 × 9.1 (7.5-12 μm. Sporocysts are oval to ellipsoidal, 5.8 (5-7 × 4.2 (3.5-5 μm.
Godwin, James; Lovich, Jeffrey E.; Ennen, Joshua R.; Kreiser, Brian R.; Folt, Brian; Lechowicz, Chris
Map turtles of the genus Graptemys are highly aquatic and rarely undergo terrestrial movements, and limited dispersal among drainages has been hypothesized to drive drainage-specific endemism and high species richness of this group in the southeastern United States. Until recently, two members of the megacephalic “pulchra clade,” Graptemys barbouri andGraptemys ernsti, were presumed to be allopatric with a gap in both species' ranges in the Choctawhatchee River drainage. In this paper, we analyzed variation in morphology (head and shell patterns) and genetics (mitochondrial DNA and microsatellite loci) from G. barbouri, G. ernsti, and Graptemys sp. collected from the Choctawhatchee River drainage, and we document the syntopic occurrence of those species and back-crossed individuals of mixed ancestry in the Choctawhatchee River drainage. Our results provide a first counter-example to the pattern of drainage-specific endemism in megacephalic Graptemys. Geologic events associated with Pliocene and Pleistocene sea level fluctuations and the existence of paleo-river systems appear to have allowed the invasion of the Choctawhatchee system by these species, and the subsequent introgression likely predates any potential human-mediated introduction.
Coelioscopy has been proven to be a valuable technique to determine the sex of juvenile chelonians. However, there is a disagreement regarding the proper way to identify testes and ovaries, which is a direct consequence of the lack of studies correlating the results of endoscopic examination with the histology of the gonad. In this blinded study we assessed two methods of sex determination in juvenile pond sliders (Trachemys scripta) using endoscopy: via coelioscopy, with visualization of the...
Espinoza, Luis L; Mertins, Omar; Gama, Gabriella S; Fernandes Patta, Ana C M; Mathews, Patrick D
A new myxosporean species, Myxidium peruviensis n. sp., is described parasitizing the gall bladder of the yellow-spotted river turtle Podocnemis unifilis kept in captivity in an Amazonian Peruvian turtle rescue unit in the city of Iquitos, State of Loreto, Peru. The parasite was found in four of ten (40%) P. unifilis examined. The new species was characterized based on morphological and molecular phylogeny analyses. SSU rDNA sequence of the spores of M. peruviensis n. sp. resulted in 1876 nucleotides and this sequence did no match any of the Myxozoa available in the GenBank. Phylogenetic analysis identified the new species as a sister species of Myxidium turturibus, the unique Myxidium species described in a Neotropical turtle. Nevertheless, the SSU rDNA sequences of the new species and M. turturibus have only a 91.5% similarity. This is the first description and molecular study of a Myxozoa in a reptile from Peru. Considering the status of P. unifilis as vulnerable species, the infection by Myxidium parasites is emphasized as possible disease impeller, representing menace to the turtle conservation. Copyright © 2017 Elsevier B.V. All rights reserved.
Pantoja-Lima, Jackson; Aride, Paulo H R; de Oliveira, Adriano T; Félix-Silva, Daniely; Pezzuti, Juarez C B; Rebêlo, George H
Consumption of turtles by natives and settlers in the Amazon and Orinoco has been widely studied in scientific communities. Accepted cultural customs and the local dietary and monetary needs need to be taken into account in conservation programs, and when implementing federal laws related to consumption and fishing methods. This study was conducted around the Purus River, a region known for the consumption and illegal trade of turtles. The objective of this study was to quantify the illegal turtle trade in Tapauá and to understand its effect on the local economy. This study was conducted in the municipality of Tapauá in the state of Amazonas, Brazil. To estimate turtle consumption, interviews were conducted over 2 consecutive years (2006 and 2007) in urban areas and isolated communities. The experimental design was randomized with respect to type of household. To study the turtle fishery and trade chain, we used snowball sampling methodology. During our study period, 100% of respondents reported consuming at least three species of turtles (Podocnemis spp.). Our estimates indicate that about 34 tons of animals are consumed annually in Tapauá along the margins of a major fishing river in the Amazon. At least five components related to the chain of commercialization of turtles on the Purus River are identified: Indigenous Apurinã and (2) residents of bordering villages (communities); (3) of local smugglers buy and sell turtles to the community in exchange for manufactured goods, and (4) regional smugglers buy in Tapauá, Lábrea, and Beruri to sell in Manaus and Manacapuru; Finally, (5) there are professional fishermen. We quantify the full impact of turtle consumption and advocate the conservation of the region's turtle populations. The Brazilian government should initiate a new turtle consumption management program which involves the opinions of consumers. With these measures the conservation of freshwater turtles in the Brazilian Amazon, is possible.
Santana Gindomar G
Full Text Available Abstract Background Throughout Brazil a large number of people seek out reptiles for their meat, leather, ornamental value and supposed medicinal importance. However, there is a dearth of information on the use of reptiles in folk medicine. In North Brazil, the freshwater turtle, Podocnemis expansa, is one of the most frequently used species in traditional medicines. Many products derived from P. expansa are utilized in rural areas and also commercialized in outdoor markets as a cure or treatment for different diseases. Here we document the use and commercialization of P. expansa for medicinal purposes in the state of Pará, Northern Brazil. Methods Data were gathered through interview-questionnaires, with some questions left open-ended. Information was collected in two localities in Pará State, North of Brazil. In the City of Belém, data was collected through interviews with 23 herbs or root sellers (13 men and 10 women. Attempts were made to interview all animal merchants in the markets visited. In fishing community of the Pesqueiro Beach, interviews were done with 41 inhabitants (23 men and 18 women and during the first contacts with the local population, we attempted to identify local people with a specialized knowledge of medicinal animal usage. Results P. expansa was traded for use in traditional medicines and cosmetics. Fat and egg shells were used to treat 16 different diseases. Turtle fat was the main product sold. The demand for these products is unknown. However, the use of this species in folk medicine might have a considerable impact on wild population, and this must be taken into account for the conservation and management of this species. Conclusion Our results indicated that the use and commercialization of P. expansa products for medicinal purposes is common in North of Brazil. More studies regarding the use and commerce of Brazilian turtles are urgently needed in order to evaluate the real impact of such activities on natural populations. We hope that our findings about the trade and use of P. expansa in folk medicine will motivate further studies on the use of animals in folk medicine and its implications for conservation.
Rossi, S; Sá-Rocha, V M; Kinoshita, D; Genoy-Puerto, A; Zwarg, T; Werneck, M R; Sá-Rocha, L C; Matushima, E R
Chelonia mydas is a sea turtle that feeds and nests on the Brazilian coast and a disease called fibropapillomatosis is a threat to this species. Because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. In order to achieve this aim, blood samples were collected from C. mydas with or without fibropapillomas captured on the São Paulo north coast. Blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile RPMI 1640 cell culture medium. Leukocytes were separated by density gradient using Ficoll-PaqueTM Plus, Amershan Biociences. The following stimuli were applied in the assessment of leukocyte function: Phorbol Miristate-Acetate (PMA) for oxidative burst activity evaluation and Zymosan A (Saccharomyces cerevisiae) Bio Particles, Alexa Fluor 594 conjugate for phagocytosis evaluation. Three cell populations were identified: heterophils, monocytes and lymphocytes. Monocytes were the cells responsible for phagocytosis and oxidative burst.
Joyce, Walter G; Werneburg, Ingmar; Lyson, Tyler R
The hooked element in the pes of turtles was historically identified by most palaeontologists and embryologists as a modified fifth metatarsal, and often used as evidence to unite turtles with other reptiles with a hooked element. Some recent embryological studies, however, revealed that this element might represent an enlarged fifth distal tarsal. We herein provide extensive new myological and developmental observations on the hooked element of turtles, and re-evaluate its primary and secondary homology using all available lines of evidence. Digital count and timing of development are uninformative. However, extensive myological, embryological and topological data are consistent with the hypothesis that the hooked element of turtles represents a fusion of the fifth distal tarsal with the fifth metatarsal, but that the fifth distal tarsal dominates the hooked element in pleurodiran turtles, whereas the fifth metatarsal dominates the hooked element of cryptodiran turtles. The term 'ansulate bone' is proposed to refer to hooked elements that result from the fusion of these two bones. The available phylogenetic and fossil data are currently insufficient to clarify the secondary homology of hooked elements within Reptilia.
Full Text Available Nest site selection for individual leatherback sea turtles, Dermochelys coriacea, is a matter of dispute. Some authors suggest that a female will tend to randomly scatter her nests to optimize clutch survival at a highly dynamic beach, while others suggest that some site fidelity exists. It is also possible that both strategies exist, depending on the characteristics of each nesting beach, with stable beaches leading to repeating nest site selections and unstable beaches leading to nest scattering. To determine the strategy of the Tortuguero population of D. coriacea, female site preference and repetition were determined by studying whether females repeat their nest zone choices between successive attempts and whether this leads to a correlation in hatching and emergence success of subsequent nests. Nesting data from 1997 to 2008 were used. Perpendicular to the coastline, open sand was preferred in general, regardless of initial choice. This shows a tendency to scatter nests and is consistent with the fact that all vertical zones had a high variability in hatching and emergence success. It is also consistent with nest success not being easily predictable, as shown by the lack of correlation in success of subsequent nesting attempts. Along the coastline, turtles showed a preference for the middle part of the studied section of beach, both at a population level and as a tendency to repeat their initial choice. Interestingly, this zone has the most artificial lights, which leads to slightly lower nest success (though not significantly so and hatchling disorientation. This finding merits further study for a possibly maladaptive trait and shows the need for increased control of artificial nesting on this beach.
Cabrera, Mario R.
Full Text Available En este trabajo se describen restos del caparazón de una tortuga que constituyen el primer registro del género dulceacuícola Trachemys Agassiz, 1857 y de la familia Emydidae Rafinesque, 1815 en la provincia de Córdoba, y el hallazgo más occidental de ambos en la Argentina. Los materiales recuperados consisten en la placa proneural, las placas pleurales IV y VIII derechas y fragmentos de las placas pleurales III y IV izquierdas, epiplastron, hioplastron e hipoplastron derechos, más tres fragmentos indeterminables. Los restos provienendel Sitio El Silencio (30°53’20" S, 62°49’29" W, un yacimiento paleontológico de edad Pleistoceno tardío - Holoceno temprano, expuesto en la costa de la actual laguna Mar Chiquita, en un nivel de limo con intercalaciones de carbonato de calcio asignado a la Formación Tezanos Pinto, cuya depositación se produjo entre los 36.000 y 8.000 AP. El registro esrelacionado con un evento húmedo acontecido entre los 16.500 y 15.500 años AP, que pudo haber sido lo suficientemente marcado y sostenido como para posibilitar el ingreso deTrachemys (y otros taxones vertebrados durante el Pleistoceno a cercanías de la laguna Mar Chiquita, unos 220 km hacia el oeste de su distribución actual.
Didiher Chacón Chaverri
Full Text Available La anidación de la tortuga baula fue estudiada en playa Gandoca, una importante playa de anidación de tortugas marinas localizada al sureste de la costa Caribeña de Costa Rica, dentro de los límites del Refugio Nacional de Vida Silvestre Gandoca/Manzanillo (82° 37' O, 09° 37' N. Entre 1990 y 1997, se desarrollaron campañas de observación de la anidación entre los meses de febrero a julio de cada año; 16 variables de la anidación fueron anotadas y una parte de la colonia fue marcada con marcas monel y 3 484 nidos fueron encontrados; durante este periodo se depositaron un promedio de 534 nidos por temporada, 1,135 nidos fue el máximo de anidación anual, mientras que 226 nidos fue el mínimo, el 20.8% de los nidos fueron relocalizados en los viveros. El promedio de la longitud curva del caparazón de las hembras fue de 154.65 cm y el promedio del ancho curvo del caparazón fue de 112.83 cm. Las observaciones mostraron que el promedio de huevos normales para este periodo fue de 79.28, mientras que el promedio de huevos vanos fue de 35. La recolección ilegal de huevos, la basura sobre la playa y la erosión intensiva son parte de los problemas más importantes que están causando el declive de las tortugas en Gandoca.The nesting of the leatherback sea turtle was studied in Gandoca Beach, an important nesting beach on the southeastern Caribbean coast of Costa Rica, in the Gandoca/Manzanillo National Wildlife Refuge. Nesting was recorded from 1990 to 1997 (each February through July. Nesting peaked in April and May (depending on the year; 16 biological paramenters were recorded and part of the rookery was tagged with monel tags. During this period 1 045 females were studied and 3 484 nests were recorded; 534 nests was the yearly mean, (range 226-1 135, 20.8 % of the nests were relocated to hatcheries, as a conservation effort to prevent loss of nests. Mean curve carapace length was 154.65 cm and width 112.83 cm. Mean normal number of eggs/nest was 79.28 and 35 yolkless. Each turtle laid an average of 2.5 nests per season with an internesting interval of 9 days. In 1997, 39% of the nesting females had been previously tagged in Gandoca Beach and a few also in Colombia (Urabá Gulf, Pacuare and Tortuguero (Costa Rica. Poaching activity, beach debris and extensive erosion represent the main hazards that are leading to a decline of the sea turtles in Gandoca.
Ana Lucia Bermúdez Romero
Full Text Available The objective of this study was to identify and describe the nesting habitat of Podocnemis sextuberculata at Erepecu Lake, Trombetas River Biological Reserve, (REBIO-Trombetas; Pará-Brazil. Initially, the main features of the beaches that potentially determine the habitat selection by cupiso for nesting were described. The nests observed on the beaches were recorded, marked and fenced as protection from natural predators. Information regarding date and location was analyzed with simple linear regression for each nest in order to determine relationships between beach features and number of nests. The results showed a positive co-relationship between number of nests and area. Nest site selection by P. sextuberculata in the beaches of the Erepecu Lake could depend on trade-off scenarios among natural threats and a suitable nesting habitat. We also suggest that, due to the high annual hydrologic oscillations on the beaches, it is possible that the driving factor for habitat selection would be the risks that the species is exposed to at the time of the search for a nesting site, rather than seeking a particular habitat type. RESUMENEl objetivo del estudio fue identificar y explicar la influencia del hábitat sobre los nidos de la tortuga “cupiso” (Podocnemis sextuberculata Cornalia, 1849, en el lago Erepecu ubicado en la Reserva Biológica del Río Trombetas (REBIO-Trombetas; Pará-Brasil. Inicialmente se describieron las principales características de las playas escogidas, los nidos encontrados fueron marcados y cercados para su protección contra la depredación natural. A través de regresiones lineales simples se determinó que el número de nidos por playa se correlacionó significativamente con el “área” de las playas. Los resultados muestran que la selección del lugar de nidificación en las playas del lago Erepecu por P. sextuberculata, podría depender en unos escenarios de compensación entre las amenazas naturales y un hábitat adecuado para anidar, ya que las condiciones ambientales de las playas del lago Erepecu son muy variantes, impidiendo que las hembras anualmente seleccionen un tipo específico de hábitat.
Joyce, Walter G; Werneburg, Ingmar; Lyson, Tyler R
The hooked element in the pes of turtles was historically identified by most palaeontologists and embryologists as a modified fifth metatarsal, and often used as evidence to unite turtles with other reptiles with a hooked element. Some recent embryological studies, however, revealed that this element might represent an enlarged fifth distal tarsal. We herein provide extensive new myological and developmental observations on the hooked element of turtles, and re-evaluate its primary and secondary homology using all available lines of evidence. Digital count and timing of development are uninformative. However, extensive myological, embryological and topological data are consistent with the hypothesis that the hooked element of turtles represents a fusion of the fifth distal tarsal with the fifth metatarsal, but that the fifth distal tarsal dominates the hooked element in pleurodiran turtles, whereas the fifth metatarsal dominates the hooked element of cryptodiran turtles. The term ‘ansulate bone’ is proposed to refer to hooked elements that result from the fusion of these two bones. The available phylogenetic and fossil data are currently insufficient to clarify the secondary homology of hooked elements within Reptilia. PMID:24102560
Lauana Schneider Fadini
Full Text Available The beaches of Espírito Santo encompass the most important nesting sites of the loggerhead sea turtle, Caretta caretta (Linnaeus, 1758, in the South Atlantic Ocean. Previous research has shown that, unlike other nesting sites in Brazil, Espírito Santo harbors a large percentage of male individuals, which play an important role in maintaining the equilibrium of C. caretta populations in the South Atlantic. During the 2006/2007 reproductive season, four beaches presenting considerable geologic differences were monitored. The sedimentary features of the nesting sites (mineral composition, albedo, and grain size, nesting dates, and clutch sizes were correlated with hatching success and incubation duration. Results show that hatching success is mostly affected by clutch size _ and to a lesser extent, by grain size and albedo. Incubation duration is more sensitive to the characteristics of the nesting site, being affected by nesting date, clutch size, mineral composition, and grain size. However, results from one nesting site cannot be generalized or extended to other sites, because sediment properties are functions of the geological framework of the area and the interaction among environmental variables is far too complex. Our results reveal the importance of protecting areas with large geologic diversity, since sedimentary characteristics may affect incubation duration and embryo survival. Our findings are important in the scope of management activities, as nest transfer may alter nest microenvironment, impacting incubation duration and hatching success.
Full Text Available Chelonia mydas is a sea turtle that feeds and nests on the Brazilian coast and a disease called fibropapillomatosis is a threat to this species. Because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. In order to achieve this aim, blood samples were collected from C. mydas with or without fibropapillomas captured on the São Paulo north coast. Blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile RPMI 1640 cell culture medium. Leukocytes were separated by density gradient using Ficoll-PaqueTM Plus, Amershan Biociences®. The following stimuli were applied in the assessment of leukocyte function: Phorbol Miristate-Acetate (PMA for oxidative burst activity evaluation and Zymosan A (Saccharomyces cerevisiae Bio Particles®, Alexa Fluor® 594 conjugate for phagocytosis evaluation. Three cell populations were identified: heterophils, monocytes and lymphocytes. Monocytes were the cells responsible for phagocytosis and oxidative burst.
María Isabel Castro-González
Full Text Available Por su potencial como fuente alimenticia, se analizó la composición química de huevo de Lepidochelys olivácea en La Escobilla, Oaxaca, México. El Instituto de Ciencias del Mar y Limnología de la Universidad Nacional Autónoma de México proporcionó 250 muestras de huevo liofilizado de 25 tortugas, que se analizaron siguiendo métodos oficiales para humedad, cenizas, proteína, grasa, lípidos totales(LT y análisis microbiológicos; además de ácidos grasos(AG por cromatografía de gases, aminoácidos(AA, vitaminas y colesterol(col por HPLC. Los resultados fueron los siguientes: (g/100g humedad(4.7, cenizas(3.8, proteína(53.7 y grasa(47.4. AA esenciales (g aa/100g Proteína: Ile (4.4, Lys(6.6, Leu(7.4, Met+Cys (8.8, Phe+Tyr (10.8; retinol (340μg/100g, colecalciferol (5.9 μg/100g, tocoferol (8.6, tiamina (0.3 y riboflavina (1.1 (mg/100g. Y los de (LT, (AG y (Col se concentraron en tres grupos por peso de tortuga: (LT (44.3-48.7-49.1g/100g, (Col (518.4-522.5-728.7 mg/100g. Entonces se identificaron 17 AGSaturados, 8 AGMonoinsaturados y 11 AGPoliinsaturados. Los AGS más abundantes (mg/100g: C16:0 (485-1263, AGM: C16:1 (456-716, C18:1n-9c (904- 1754 y AGP: C20:4n-6 (105-217, EPA (48-103 y HA (97-189. También, existió diferencia significativa (Fisher, p<0.05 para (Col, AG totales, AGS, AGM, AGP y AG n-3 (EPA+DHA. No se detectó presencia de agentes microbiológicos. El huevo liofilizado de L. olivacea es una opción para el desarrollo de productos alimenticios por su proteína de alto valor biológico y ácidos grasos n-3.
LESLIE ANAIS CASTILLO-CUTIVA
Full Text Available Se realizó la caracterización de la diversidad genética de la población de Rhinoclemmys nasuta, que habita en la localidad insular de Isla Palma, Bahía Málaga (Valle del Cauca, utilizando tres sistemas de microsatélites (Cm72, Cm58 y Cm3. En esta localidad, R. nasuta se encuentra ampliamente distribuida en los sistemas de riachuelos y quebradas presentes. Se tomaron entre 100 a 200 μL de sangre periférica de diez tortugas en cinco riachuelos, preservando las muestras en una solución 0,5 M de EDTA y el ADN fue extraído mediante las técnicas de Salting-out y Chelex. Los productos amplificados por PCR fueron visualizados y medidos en geles de poliacrilamida teñidos con nitrato de plata. Se obtuvieron productos amplificados exitosos para todos los sistemas analizados, dos de los cuales (Cm72 y Cm3 resultaron ser monomórficos, mientras que el sistema Cm58 presentó un PIC alto (0,698 permitiendo estimar la diversidad genética de esta población. La heterocigosidad observada fue baja (Ho = 0,26 y los índices de endogamia FIS y FIT fueron altos (0,67857 y 0,67881, indicando un exceso de homocigotos en cada uno de los ríos y para la población total. El Análisis Molecular de Varianza sugirió que no existe estructura genética diferencial en esta población (FST = 0,00075; p = 0,95112. En conclusión los resultados sugieren que la diversidad genética de la población de R. nasuta en Isla Palma es baja, siendo una población altamente endogámica
Werneck M. R.
Full Text Available We report the occurrence of Neospirorchis schistosomatoides Price 1934 in a juvenile green turtle (Chelonia mydas L.1758 from the Brazilian coast. This species has been reported only in green turtles from the USA, Bermuda and Australia. Only two entire specimens and parts of six worms were found in the host’s heart. The collection, identification and morphometric data of intact specimens of N. schistosomatoides are rare and restricted to the original description. This paper presents the first report of N. schistosomatoides in green turtles from the western South Atlantic Ocean.
Abstract We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona,...
Murphy, Robert W.; Berry, Kristin H.; Edwards, Taylor; Leviton, Alan E.; Lathrop, Amy; Riedle, J. Daren
Abstract We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection. PMID:21976992
Full Text Available We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii Cooper. The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of G. lepidocephalus Ottley et Velázques Solis, 1989 from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because G. lepidocephalus is from the Mojavian population no names are available for the Sonoran species. Thus, a new species, Gopherus morafkai sp. n., is named and this action reduces the distribution of G. agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of G. agassizii, which may deserve a higher level of protection.
Murphy, Robert W; Berry, Kristin H; Edwards, Taylor; Leviton, Alan E; Lathrop, Amy; Riedle, J Daren
We investigate a cornucopia of problems associated with the identity of the desert tortoise, Gopherus agassizii (Cooper). The date of publication is found to be 1861, rather than 1863. Only one of the three original cotypes exists, and it is designated as the lectotype of the species. Another cotype is found to have been destroyed in the 1906 San Francisco earthquake and subsequent fire. The third is lost. The lectotype is genetically confirmed to be from California, and not Arizona, USA as sometimes reported. Maternally, the holotype of Gopherus lepidocephalus (Ottley & Velázques Solis. 1989) from the Cape Region of Baja California Sur, Mexico is also from the Mojavian population of the desert tortoise, and not from Tiburon Island, Sonora, Mexico as previously proposed. A suite of characters serve to diagnose tortoises west and north of the Colorado River, the Mojavian population, from those east and south of the river in Arizona, USA, and Sonora and Sinaloa, Mexico, the Sonoran population. Species recognition is warranted and because Gopherus lepidocephalus is from the Mojavian population, no names are available for the Sonoran species. Thus, a new species, Gopherus morafkaisp. n., is named and this action reduces the distribution of Gopherus agassizii to only 30% of its former range. This reduction has important implications for the conservation and protection of Gopherus agassizii, which may deserve a higher level of protection.
Parris, L.B.; Lamont, M.M.; Carthy, R.R.
Hatching sea turtles may be at risk to fire ant predation during egg incubation, and especially at risk once pipped from the egg, prior to hatchling emergence from the nest. In addition to direct mortality, fire ants have the potential to inflict debilitating injuries that may directly affect survival of the young. The increased incidence of red imported fire ant induced mortality and envenomization of loggerhead sea turtle hatchlings on Cape San Blas suggests this invasive ant species may pose a serious threat to the future of this genetically distinct population.
Hartog, den J.C.; Nierop, van M.M.
Microscopical investigations of the gut contents of six individuals of Dermochelys coriacea from southern England and the North Sea revealed the presence in all of these of numerous nematocysts, mainly scyphozoan. Only six species of Scyphozoa occur in British shallow waters and in the North Sea,
Matthew J Spanier
Full Text Available Leatherback sea turtles (Dermochelys coriacea nest on dynamic, erosion-prone beaches. Erosive processes and resulting nest loss have long been presumed to be a hindrance to clutch survival. In order to better understand how leatherbacks cope with unstable nesting beaches, I investigated the role of beach erosion in leatherback nest site selection at Playa Gandoca, Costa Rica. I also examined the potential effect of nest relocation, a conservation strategy in place at Playa Gandoca to prevent nest loss to erosion, on the temperature of incubating clutches. I monitored changes in beach structure as a result of erosion at natural nest sites during the time the nest was laid, as well as in subsequent weeks. To investigate slope as a cue for nest site selection, I measured the slope of the beach where turtles ascended from the sea to nest, as well as the slopes at other random locations on the beach for comparison. I examined temperature differences between natural and relocated nest sites with thermocouples placed in the sand at depths typical of leatherback nests. Nests were distributed non-randomly in a clumped distribution along the length of the beach and laid at locations that were not undergoing erosion. The slope at nest sites was significantly different than at randomly chosen locations on the beach. The sand temperature at nest depths was significantly warmer at natural nest sites than at locations of relocated nests. The findings of this study suggest leatherbacks actively select nest sites that are not undergoing erosive processes, with slope potentially being used as a cue for site selection. The relocation of nests appears to be inadvertently cooling the nest environment. Due to the fact that leatherback clutches undergo temperaturedependent sex determination, the relocation of nests may be producing an unnatural male biasing of hatchlings. The results of this study suggest that the necessity of relocation practices, largely in place to protect nests from erosion, should be reevaluated to ensure the proper conservation of this critically endangered species. Rev. Biol. Trop. 58 (4: 1237-1246. Epub 2010 December 01.
Full Text Available El presente trabajo registra por primera vez para el Perú a Nematophila grandis (Diesing, 1839 Travassos, 1934, en la tortuga de río Podocnemis unifilis «taricaya». Los hospederos fueron colectados en las localidades del Río Putumayo, Samiria, Iquitos (Loreto y Manu (Madre de Dios. El material identificado pertenece a la Colección Helmintológica del Departamento de Protozoología, Helmintología e Invertebrados Afines del Museo de Historia Natural de la Universidad Nacional Mayor de San Marcos.
Gibbons Lynda M
Full Text Available Specimens collected from the large intestine of the sidenecked turtle Podocnemis unifilis Troschel, 1848 in the region of Cuminá and Trombetas rivers near Pará, Brazil are assigned to a new genus and new species of the nematode superfamily Cosmocercoidea and family Atractidae and named Paraorientatractis semiannulata. The new genus is separated from the nearest genus Orientatractis by the funnelshaped mouth opening, the presence of 4 distinct lips, 4 papillae in the internal cycle, one on each lip margin, 2 lateral amphids with large amphidial pores and absence of submedian papillae. It is also separated from Orientatractis and Proatractis by the presence of striated lateral alae which curve dorsally extending from mid oesophagus to mid tail, the difference in size of the vulvar opening and the presence of large transverse ridges or semiannules on the dorsal surface. The new species can be separated from the species of the genera Orientatractis and Proatractis by the characters that distinguish the genera and the arrangement of the caudal papillae on the male. A host/parasite list for Podocnemis spp. is included
Background. Neochelys franzeni Schleich, 1993 is the only pleurodire or side-necked turtle from the middle Eocene, Messel Pit (the first UNESCO, World Natural Heritage Site in Germany, since 1995). The original description of the species is based on two specimens SMF ME 1091 (Holotype) and 715 (Paratype) housed at the Senckenberg Museum Frankfurt. The excellent preservation of complete and articulated skeletons of this species makes it a key taxon for understanding the evolution and phylogeny of the European Neochelys genus and its relationships with South American and African-Madagascar podocnemidids. Results. Five new specimens of Neochelys franzeni from Messel Pit are described here, together with the redescription of SMF ME 1091 and 715. Specimens correspond to individuals of different ontogenetic stages showing conservative morphology from hatching to adults. A revised diagnosis for the species is presented here, together with its inclusion in a global phylogenetic analysis of Pelomedusoides that shows that this species and the whole Neochelys spp. is sister to the Erymnochelys madagascariensis-Peltocephalus dumerilianus clade within Podocnemididae.
Full Text Available Rhinoclemmys punctularia punctularia is a semi-aquatic chelonian found in Northern South America. We analyzed the habitat use, size structure and sex ratio of the species on Algodoal-Maiandeua Island, a protected area on the Northeastern coast of the Brazilian state of Pará. Four distinct habitats (coastal plain lake, flooded forest “igapó”, interdunal lakes, and tidal channels were surveyed during the rainy (March and April and dry (August and September seasons of 2009, using hoop traps. For the analysis of population structure, additional data were taken in March and August, 2008. A total of 169 individuals were captured in flooded forest (igapó, lakes of the coastal plain and, occasionally, in temporary pools. Capture rates were highest in the coastal plain lake, possibly due to the greater availability of the fruits that form part of the diet of R. p. punctularia. Of the physical-chemical variables measured, salinity appeared to be the only factor to have a significant negative effect on capture rates. The sex ratio was only slightly biased to females, and did not vary between habitats or seasons. Straight carapace length was significantly larger in females, but did not vary between habitats. Overall, the evidence indicates that both biotic and abiotic factors like food availability, low current and salinity, influence the habitats selection and use by R. p. punctularia on Algodoal-Maiandeua Island.Rhinoclemmys punctularia punctularia es un quelonio semi-acuático, con amplia distribución geográfica. El presente trabajo analiza la densidad relativa, proporción sexual y el uso de hábitat de esta especie en la isla de Algodoal-Maiandeua, en el litoral Norte de Brasil. Cuatro hábitats distintos fueron muestreados durante los períodos de lluvias y seco de 2009, en donde se utilizaron trampas de aro. Muestras de 2008 (marzo-agosto también se emplearon para el análisis de la estructura de la población. Asimismo, fueron encontrados 169 ejemplares en el bosque inundado, lagunas de tierras bajas y, ocasionalmente, en lagunas temporales. Las tasas de captura fueron más altas en las lagunas costeras, posiblemente debido a la mayor disponibilidad de frutos, que sirven de alimento para esta especie. De las variables físico-químicas, la salinidad fue el único factor que se correlaciona inversamente con las tasas de captura. La proporción de sexos fue ligeramente superior en las hembras, pero no varió entre los hábitats o período del año. El tamaño promedio de los individuos fue mayor en las hembras, pero no varió entre hábitats. Los factores ambientales, como la disponibilidad de alimentos, la baja salinidad y la poca corriente, probablemente hayan favorecido la selección y el uso de estos hábitats por R. p. punctularia.
Vlachos, Evangelos; Cerda, Ignacio; Tsoukala, Evangelia
Soft-shelled turtles (Pan-Trionychidae) are not included in the present-day chelonian fauna of Greece and have been unknown in the Greek fossil record up to now. Here, we report the first fossil occurrence of a soft-shelled turtle from Greece, originating from the Pliocene Gefira Member (Angelochori Formation), in the lower Axios valley. The corresponding specimens were discovered with several mammalian remains, most of them attributable to the mastodon of Auvergne, Anancus arvernensis. The chelonian material includes five carapacial fragments that belong to the same individual and can be attributed to Pan-Trionychidae based on the typical sculpturing on the dorsal side of the carapace. Most of these bony plates were histologically sampled and thereby provide evidence for the "plywood" structure, another characteristic of pan-trionychids. They represent the first extended sampling of trionychid plates that belong to the same individual, allowing the documentation of the variation of the relevant trionychid morphologies in the carapace. These findings expand the paleobiogeographic range of this taxon to the southern Balkans and Greece and allow a better estimation of the chelonian paleo-fauna of the area. They are also important for the temporal distribution of this clade in the Paleoarctic, as they join specimens from Italy as being the last trionychids in Europe.
Braga, Heitor de Oliveira; Schiavetti, Alexandre
The use of ethnoecological tools to evaluate possible damage and loss of biodiversity related to the populations of species under some degree of threat may represent a first step towards integrating the political management of natural resources and conservation strategies. From this perspective, this study investigates fishermen's ecological knowledge about sea turtles and attitudes towards the conservation and bycatch in Ilhéus, Southern Bahia, Brazil. Fishermen experts semi-structured interviews were performed using snowball sampling method. The interviews consisted of a series of questions relating to the fishermen's profile, structure and work equipment, the local ecological knowledge of fishermen about sea turtles and bycatch, a projective test, attitudes towards turtle conservation and beliefs and taboos regarding turtles. Indicators for quantitative comparisons of respondents in terms of their broad knowledge and attitudes towards turtle conservation were created. Correlation analyses were made between indicators of knowledge and attitude as well as the relationship between education level and knowledge and attitudes. Thirty experts were interviewed for the study. The local ecological knowledge and attitudes of fishermen towards the conservation of sea turtles were respectively medium (0.43) and moderate (0.69) according to experts (based on Likert scale and Cronbach's Alpha). Potential areas of spawning were reported from Barra Grande to Una covering the entire coast of Ilhéus. Methods for identifying the animal, behavior, and popular names were described by fishermen. The most recent captures of turtles were attributed to fishing line, but according to the respondents, lobster nets and shrimp traps are more likely to capture turtles. Knowledge and attitudes were weakly inversely correlated (r = -0.38, p = 0.04), and the education level of the respondent showed a positive correlation with positive attitudes towards turtle conservation (H = 8.33; p = 0.04). Life history, habitat, specific and exogenous taboos, beliefs and the use of hawksbill turtle to make glasses and other handcrafts are also reported in the study. Monitoring of spawning areas, preservation of traditional practices, strategies to moderate the use of fishery resources and the local ecological knowledge/attitudes can provide data to improve the conservation practices and management of sea turtles.
Hartog, den J.C.; Nierop, van M.M.
Microscopical investigations of the gut contents of six individuals of Dermochelys coriacea from southern England and the North Sea revealed the presence in all of these of numerous nematocysts, mainly scyphozoan. Only six species of Scyphozoa occur in British shallow waters and in the North Sea, vi
Werneck M. R.
Full Text Available This note reports the occurrence of Hapalotrema postorchis Rao 1976 collected from an aortic aneurysm in a green turtle (Chelonia mydas Linnaeus 1758 found on the coast of Brazil. Besides the vascular lesion, granulomatous reactions with foreign - body giant cells were found surrounding the parasite eggs in the heart, intestines, liver, pancreas, spleen, brain and kidneys. This paper presents the first report of H. postorchis and accompanying lesions in a green sea turtle from the western South Atlantic Ocean.
Werneck M. R.
Full Text Available This paper describes the occurrence of Enodiotrema megachondrus (Looss, 1899 Looss, 1901 in a juvenile green sea turtle (Chelonia mydas Linnaeus, 1758 found on the coast of Brazil. This parasite has been described in Caretta caretta from Egypt, France, the Mediterranean Sea, the Madeira Archipelago, the Adriatic Sea and the USA, in C. mydas from Egypt and the USA, in Eretmochelys imbricata from Cuba, in Lepidochelys olivacea from Mexico and Costa Rica and in Lepidochelys kempii from USA. This note represents the first report of E. megachondus in a green sea turtle in the South-West Atlantic Ocean.
Elizabeth A. Lacey
Full Text Available Green sea turtles, Chelonia mydas, are grazers influencing the distribution of seagrass within shallow coastal ecosystems, yet the drivers behind C. mydas patch use within seagrass beds are largely unknown. Current theories center on food quality (nutrient content as the plant responds to grazing disturbances; however, no study has monitored these parameters in a natural setting without grazer manipulation. To determine the morphological and physiological responses potentially influencing seagrass recovery from grazing disturbances, seagrasses were monitored for one year under three different grazing scenarios (turtle grazed, fish grazed and ungrazed in a tropical ecosystem in Akumal Bay, Quintana Roo, Mexico. Significantly less soluble carbohydrates and increased nitrogen and phosphorus content in Thalassia testudinum were indicative of the stresses placed on seagrasses during herbivory. To determine if these physiological responses were the drivers of the heterogeneous grazing behavior by C. mydas recorded in Akumal Bay, patches were mapped and monitored over a six-month interval. The abandoned patches had the lowest standing crop rather than leaf nutrient or rhizome soluble carbohydrate content. This suggests a modified Giving Up Density (GUD behavior: the critical threshold where cost of continued grazing does not provide minimum nutrients, therefore, new patches must be utilized, explains resource abandonment and mechanism behind C. mydas grazing. This study is the first to apply GUD theory, often applied in terrestrial literature, to explain marine herbivore grazing behavior.
Full Text Available Background Abundant pan-trionychid (soft-shell turtles specimens have been found in Eocene sequences of central Europe, particularly from two localities in Germany, the Messel Pit (a UNESCO World Natural Heritage Site and Geiseltal, traditionally attributed to Trionyx messelianus or Rafetoides austriacus. Over the last two decades new specimens of this taxon from these two localities have been discovered and fully prepared. However, they have remained unstudied, as well as their phylogenetic position inside Pan-Trionychidae is unknown. Results Five new specimens of Palaeoamyda messeliana nov. comb. from Messel Pit and Geiseltal localities are fully described here. A revised diagnosis for the species is also presented here, together with its inclusion in a phylogenetic analysis of Pan-Trionychidae that shows that this species is sister to the extant Amyda cartilaginea, one of the most abundant pan-trionychid (soft-shell turtles from Asia, both members of the clade Chitrini. The specimens described in here are among the best and most complete fossil pan-trionychid skeletons so far known.
PD. Ferreira Júnior
Full Text Available Nest site has influence on incubation duration and hatching success of two Neotropical turtles, the Giant Amazon River Turtle (Podocnemis expansa and Yellow-Spotted Side-Neck Turtle (Podocnemis unifilis - "Tracajá". The 2000 and 2001 nesting seasons have been monitored at the Javaés River in Bananal Island, Brazil. Although they nest on the same beaches, there is a separation of the nesting areas of P unifilis and P. expansa nests on the upper parts of the beach. The incubation duration for P. expansa is influenced by the nesting period, the height of the nest from the river, the clutch size, and the grain size in the site of the nest. Nests of Podocnemis expansa placed in coarse sediments have shorter incubation duration than those placed in finer sediments. The hatching success in P. expansa is influenced by grain size, incubation duration, and nesting period. The grain size is negatively correlated with hatching success, indicating that the nests situated in finer-grained sand have better chances of successful egg hatching than those in coarser-grained sand. Nests of the end of the reproductive season have lower hatching success and incubation duration than those at the start of the season. For P. unifilis, the nesting period and nest depth influence the incubation duration; moreover, the river dynamics significantly affect the hatching success. The oscillation of the river level and the moment of initial increase, the height of the nest from the river level, and the nesting period are all decisive components for hatching success. The results of this research show the importance of protecting areas with great geological diversity, wherein the features of the environment can affect the microenvironment of nests, with consequences on incubation duration and hatching success.
Full Text Available Rhinoclemmys nasuta (Boulenger 1902 es una especie endémica que se encuentra registrada en el libro rojo de los reptiles de Colombia y está catalogada como casi amenazada a nivel mundial. Esta especie, de tamaño mediano, habita los cursos de agua en la región Tumbes-Chocó, desde la cuenca del río Esmeraldas (Ecuador hasta la zona media de la cuenca del río Atrato (Chocó, Colombia. Hasta la fecha no se ha podido realizar una adecuada valoración de su estado de conservación debido a diferentes vacíos de conocimientos identificados con relación a la historia natural y la ecología de la especie. En el presente trabajo se recogen los resultados del análisis del tamaño y estructura de una población de R. nasuta estudiada en un ambiente insular del Pacífico colombiano (Isla Palma, Bahía Málaga, Valle del Cauca. En las condiciones de estudio, la población estuvo dominada por individuos adultos siendo la proporción Hembras: Machos: Juveniles de 1.00: 0.71: 0.85. Las hembras fueron consistentemente más grandes que los machos (♀: 179.87 ± 3.27 mm; ♂:151.83 ± 2.41 mm, definiéndose un índice general de dimorfismo sexual de 1.18. Con base en los registros de captura-recaptura, se estimó que el tamaño de la población de esta especie en Isla Palma durante el periodo de estudio fue de 990 individuos (IC90%: 941 a 1044 ind, con una densidad promedio de 2444 ± 122 tortugas por cada hectárea de río. Al evaluar la relación tamaño-peso, se estableció que el crecimiento de R. nasuta es de tipo isométrico (b = 3.04, siendo la tendencia de crecimiento similar entre machos y hembras a pesar del dimorfismo sexual detectado. Este trabajo es el primer esfuerzo de investigación en la región pacífica de Colombia que aborda el estudio sistemático de una población de tortugas continentales. Además, proporciona información sobre la historia natural de R. nasuta que fortalecerá las iniciativas de conservación de tortugas en Colombia.
Full Text Available 96% de la postura anual de Trachemys scripta ornata sucede entre los meses de noviem- bre hasta marzo con frecuencias máximas de oviposición en diciembre (34% y enero (23% correspondientes al período seco del año. No existe asociación (correlación de Pearson entre porcentaje de posturas promedio/mes vs. precipitación, humedad relativa o temperatura (ρ = -0,23; ρ = -0,08; ρ = 0,02 pero es positiva en relación con horas luz/día (ρ = 0,42.
Braga, Heitor de Oliveira; Schiavetti, Alexandre
... the political management of natural resources and conservation strategies. From this perspective, this study investigates fishermen's ecological knowledge about sea turtles and attitudes towards the conservation and bycatch...
Parasitic Ulcerous Caseous Gastroesophagitis Associated with Rameshwarotrema uterocrescens Rao, 1975 (Digenea: Pronocephalidae) in a Juvenile Green Turtle [Chelonia mydas, Linnaeus 1758 (Testudines: Cheloniidae)]: A Case Report.
Ribeiro, Rachel B; Jerdy, Hassan; Wernek, Max R; Goldberg, Daphne W; Bianchi, Mariah; Carvalho, EulÃ Â Gio C Q
Here we report a case of ulcerative caseous gastroesophagitis associated with Rameshwarotrema uterocrescens; Rao, 1975 (Digenea: Pronocephalidae) in a juvenile green turtle (Chelonia mydas) from Southern Brazil. Similar pathologies have been reported only in adult green turtles from Costa Rica. This paper presents the second report of parasitic esophagitis due R. uterocrescens and the first occurrence in juvenile green turtles along coastal Brazil.
Ana L. Abreu-Silva
Full Text Available O objetivo do presente trabalho foi analisar histológica e histo- químicamente a pele do jurará (Kinosternon scorpioides scorpioides. Foram utilizados seis animais (três machos e três fêmeas. Os animais foram eutanasiados com dose letal de tiopental sódico a 2,5%, para colheita de fragmentos de pele mole das patas e pescoço do animal, que após a fixação em líquido de Bouin, foram incluídos em parafina e corados pelas técnicas de hematoxilina-eosina, Giemsa, Sirius red, Reticulina de Gomori e Fucsina-resorcina de Weigert. Os resultados revelaram que a pele do jurará é delgada e composta de epiderme e derme. A epiderme é formada por estrato germinativo constituído por uma única camada de células cilíndricas; estrato espinhoso apresentando duas ou três camadas de células poliédricas; o estrato granuloso não foi observado nos exemplares estudados O estrato córneo apresenta uma delgada camada de queratina mole. Na derme, os fibroblastos foram as células mais freqüentes e as fibras colágenas formavam feixes espessos dispostos em várias direções. No método do Picro Sirius Red sob luz polarizada observou-se que, independente da região analisada, há predomínio de fibras colágenas tipo I em relação ao colágeno tipo III. Foi também observados mastócitos em pequena quantidade e fibras elásticas na região subepidérmica. Concluiu-se que a pele de Kinosternon scorpioides scorpioides possui características semelhantes a dos demais vertebrados (anfíbios, aves e mamíferos, apresenta peculiaridades, como por exemplo, a ausência de papilas dérmicas e glândulas.
de Morais, Paula Benevides; de Souza, Denise Rodrigues; de Sousa, Francisca Maria Pinheiro; de Oliveira, Kleverson Wessel; Pimenta, Raphael Sanzio
Shigella flexnerii and Escherichia coli were the most frequent Gram-negative bacteria found in the mouth cavity and cloacae of the turtles Podocnemis expansa and P. unifilis on beaches in the National Park of Araguaia, Brazil. Reptiles are known as Salmonella carriers, despite rarely isolated in these turtles. PMID:24031664
Full Text Available New fossil vertebrates from the most representative Upper Pleistocene section (Tyrrhenian, MIS 5e of the outcrop of San Giovanni di Sinis (Oristano, Sardinia are here reported and described. The fossils, although scarce and fragmentary, document the occurrence of a terrapin (Mauremys sp. and the endemic Sardinian deer (Praemegaceros cazioti. Significant is the occurrence of the terrapin because it is the youngest representative of the genus in the central Mediterranean area where it is extinct at present. The Late Pleistocene extinction of Mauremys in Italy follows the same pattern of other Mediterranean reptiles, in being in some cases delayed on the islands. A comparison of the modern range of Mauremys and that of the pond turtle, Emys, as well as of their past ranges as evidenced by the fossil record, might suggest that some sort of thermophily (at least during pre-hatching stages characterized the former taxon and is responsible for its past and present distribution. SHORT NOTE
Hartog, den J.C.; Nierop, van M.M.
Microscopical investigations of the gut contents of six individuals of Dermochelys coriacea from southern England and the North Sea revealed the presence in all of these of numerous nematocysts, mainly scyphozoan. Only six species of Scyphozoa occur in British shallow waters and in the North Sea, vi
Avaliação da predação de Podocnemis expansa e Podocnemis unifilis (Testudines, Podocnemididae no rio Javaés, Tocantins Evaluation of predation in Podocnemis expansa and Podocnemis unifilis (Testudines, Podocnemididae in the Javaés River, Tocantins
Giovanni Salera Junior
Full Text Available Podocnemis expansa e P. unifilis são animais de vida longa, com uma demorada maturação sexual, o que influencia uma baixa taxa de substituição de indivíduos. Suas populações são caracterizadas por uma pequena mortalidade dos animais adultos, mas alta taxa de mortalidade de filhotes e embriões. Sendo a predação natural de ninhos e filhotes um dos fatores mais importantes do baixo sucesso de eclosão dessas espécies. No rio Javaés, os ovos e recém-eclodidos podem ser predados por uma grande diversidade de animais: dentre as aves, urubus (Coragyps atratus e Cathartes aura, carcará (Polyborus plancus, jaburu (Jabiru mycteria; lagartos (Tupinambis teguixin e mamíferos de pequeno porte, coati (Nasua nasua e cachorro-do-mato (Cerdocyon thous. Do total anual de desovas de P. unifilis em média 65,98% são predadas, sendo 41,68% de forma total e 24,30% parcialmente. Enquanto que apenas 5,31% das ninhadas de P. expansa são sempre parcialmente predadas. Dentre os predadores aquáticos existem diversos peixes, principalmente piranhas (Serrasalmus nattereri e jacarés (Melanosuchus niger e Caimam crocodilus. Os predadores das fêmeas de P. unifilis são: jacaré-açu (Melanosuchus niger, onça-pintada (Panthera onca e onça-parda (Puma concolor. Enquanto que as fêmeas de P. expansa em postura, somente são predadas por P. onca. As fêmeas de P. unifilis em postura são predadas num total médio de 3,93% anualmente, enquanto que para P. expansa a média anual é 5,66% das fêmeas.Podocnemis expansa and P. unifilis long lived with late sexual maturation, which influences a low replacement rate of individuals. Their populations are characterized by low adults mortality, but high mortality of embryos and hatchlings. The natural nest predation is an important factor for hatchling success. In Javaés River, the eggs and hatchlings can be predated by a large number of animals such as birds, vultures (Coragyps atratus and Cathartes aura, carcará (Polyborus plancus, jabiru (Jabiru mycteria; lizards (Tupinambis teguixin and small mammals, coati (Nasua nasua and crab-eating-fox (Cerdocyon thous. About 65,98% of nests of P. unifilis are predated (41,68% totally and 24,30% partially. Only 5,31% of P. expansa nests are partially predated. There are many aquatic predators, like carnivorous fish, mainly piranhas (Serrasalmus nattereri and caimans (Melanosuchus niger e Caimam crocodilus. The predators of P. unifilis females are: black caiman (Melanosuchus niger, jaguar (Panthera onca and puma (Puma concolor, and of P. expansa females is the P. onca. In average 3,93% of P. unifilis females are predated annually while that to P. expansa the average is 5,66%.
Morfometria e histologia das gônadas de machos e fêmeas recém-eclodidos de Podocnemis expansa e Podocnemis unifilis (Testudines, Podocnemididae=Morphometry and histology of the gonads of males and females hatchlings of Podocnemis expansa and Podocnemis. unifilis (Testudines, Podocnemididae
Adson Gomes de Ataídes
Full Text Available Considerando a importância da técnica histológica na sexagem de quelônios e os poucos trabalhos direcionados para esta técnica, é proposto neste estudo caracterizar morfometricamente e histologicamente as gônadas de P. expansa e P. unifilis. Todos os espécimes utilizados neste trabalho foram procedentes do rio Javaés, entorno do Parque Nacional do Araguaia, Estado do Tocantins, Brasil. Após estudo sob estereomicroscópio, foram coletadas as gônadas para o procedimento histológico de rotina. Foram analisadas 187 amostras de P. expansa, das quais 81,2% foram identificadas como fêmeas e 18,7% como machos; e 98 de P. unifilis, das quais 31,6% eram fêmeas e 68,4% machos. Não foram verificadas diferenças microscópicas relevantes na sexagem entre estas espécies. Os critérios utilizados no diagnóstico microscópico foram principalmente a morfologia das gônadas, a presença do apêndice no oviduto remanescente, a morfologia do oviduto e as características das células de revestimento dos folículos e túbulos seminíferos. Quando comparadas as medidas biométricas dos ovários e testículos, somente foram observadas diferenças para a largura. Considering the importance of the histological sexing technique of turtles and the few works directed to this objective, it was proposed in this study to morphometrically and histologically characterize the gonads of P. unifilis and P. expansa. All specimens used in this work originated in the Javaés river, around the Araguaia National Park, Tocantins State, Brazil. Following a study under a stereoscope, the gonads were collected for the routine histological procedure. A total of 187 samples of P. expansa were analyzed, of which 81.2% were identified as female and 18.7% as male; as well as 98 P. unifilis samples, of which 31.6% were female and 68.4% males. No relevant microscopic differences were verified in the sexing between these species. The criteria used in the microscopically diagnosis were primarily gonad morphology, presence of the appendix in the remaining oviduct, oviduct morphology and the characteristics of covering cells of the follicles and somniferous tubules. When the measurements of the ovaries and testicles were compared, only differences in width were observed.
Comportamento e preferência alimentar em Podocnemis expansa (Schweigger, P. unifilis (Troschel e P. sextuberculata (Cornalia em cativeiro (Testudines, Pelomedusidae Feeding behavior and food preference of Podocnemis expansa (Schweigger, P. unifilis (Troschel and P. sextuberculata (Cornalia in captivity (Testudines, Pelomedusidae
Full Text Available The feeding behavior and the food preference was studied in P. expansa (Schweigger, 1812 P. unifilis (Troschel, 1848 and P. sextuberculata (Cornalia, 1849. The method used for feeding behavior and food preference was the sampling of all occurrences. The Students' t test was applied on the food items, to compare differences during the development of each species. The main conclusions are: feeding behavior for food, except alive animals, is divided in foraging, approach, olfactory recognition, capture, laceration and ingestion; the persecution behavior was observed for prey activity; cleptoparasitism occurs in the three species and neustophagia mechanism was detected in P. unifilis; P. sextuberculata shows to be almost only carnivorous and P. expansa and P. unifilis show to be omnivorous; P. expansa can be considered more herbivorous in captivity than P. unifilis during the age between one and five years old and more than five years old; P. expansa is more sensitive than P. unifilis concerning the alteration of the food place, mainly the meat, decreasing its consumption, if it is put on a dry place.
Description of behavioral patterns of Podocnemis erythrocephala (Spix, 1824 (Testudines: Podocnemididae (Red-headed river turtle in captivity, Manaus, Amazonas, Brazil Descrição de padrões comportamentais de Podocnemis erythrocephala (Spix, 1824 (Testudines: Podocnemididae (Irapuca em cativeiro, Manaus, Amazonas, Brasil
Full Text Available The biology and ecology of South American turtles is still poorly known, particularly, for the Brazilian species. Laboratory studies are essential to understand the life cycles of aquatic turtles species and to help in formulating management plans for their conservation. As a contribution to the knowledge of Podocnemis erythrocephala species, we give a description of its species-typical behaviors, categorized as: maintenance, locomotion, feeding, agonistic and reproduction, based on captives observations of four pairs of turtles in an aquarium in Manaus, Brazil. Similarities and differences with the repertoires of other turtle species are discussed, concluding that turtles have much more complex adaptative strategies and social life than was believed.A biologia e ecologia dos quelônios da América do Sul é pouco conhecida, particularmente para as espécies brasileiras. Estudos em laboratório são essenciais para entender o ciclo de vida das espécies de tartarugas aquáticas e ajudar na formulação de planos de manejo para sua conservação. Como uma contribuição ao conhecimento da espécie Podocnemis erythrocephala, nós descrevemos os comportamentos típicos nas categorias manutenção, locomoção, alimentação, agonístico e reprodutivo, baseado em observações de quatro pares de tartarugas em um aquário na cidade de Manaus, Brasil. São discutidas similaridades e diferenças com repertórios de outras espécies de quelônios, concluindo que esses animais possuem estratégias adaptativas e vida social muito mais complexas do que tem sido ponderado.
Radiographic anatomy aspects and gastrointestinal transit time in Podocnemis unifilis troschel, 1848 (Testudines, Podocnemididae - doi: 10.4025/actascibiolsci.v32i4.5082 Radiographic anatomy aspects and gastrointestinal transit time in Podocnemis unifilis troschel, 1848 (Testudines, Podocnemididae. - doi: 10.4025/actascibiolsci.v32i4.5082
Fernando Moraes Machado Brito
Full Text Available The present study analyzed the radiographic anatomy and determined the gastrointestinal transit time of Podocnemis unifilis. We used ten animals belonging to LAPAS from the Federal University of Uberlândia, Uberlândia, Minas Gerais State, Brazil. The animals were orally fed with a barium sulphate suspension at 10 mL kg-1 mixed with mineral oil, at a ratio of 70% of barium sulphate for 30% of mineral oil. Afterwards, the animals underwent radiography in a dorsum ventriloquoal position, with the X-ray device adjusted at 72 Kv and 200 mA, in time intervals to follow the permanency of contrast in the organism. Five minutes after the contrast was supplied, the stomach was filled. After sixteen hours the contrast advanced to the small intestine. In 48 hours, the whole small intestine and part of the colon were fulfilled. On the 9th day the stomach was empty and the contrast advanced to the colon. On the 11 th day, the colon was totally fulfilled, and the contrast was close to cloaca. On the 18th day all contrast was eliminated by the animal. Total time for contrast elimination was, in average, 17.6 ± 2.4 days, with the minimum of 12 and maximum of 22 days, with temperature at 27°C. The digestion of the food was slower in the duodenum, and faster in the colon-rectum, which presents lower indices of repletion.The present study analyzed the radiographic anatomy and determined the gastrointestinal transit time of Podocnemis unifilis. We used ten animals belonging to LAPAS from the Federal University of Uberlândia, Uberlândia, Minas Gerais State, Brazil. The animals were orally fed with a barium sulphate suspension at 10 mL kg-1 mixed with mineral oil, at a ratio of 70% of barium sulphate for 30% of mineral oil. Afterwards, the animals underwent radiography in a dorsum ventriloquoal position, with the X-ray device adjusted at 72 Kv and 200 mA, in time intervals to follow the permanency of contrast in the organism. Five minutes after the contrast was supplied, the stomach was filled. After sixteen hours the contrast advanced to the small intestine. In 48 hours, the whole small intestine and part of the colon were fulfilled. On the 9th day the stomach was empty and the contrast advanced to the colon. On the 11 th day, the colon was totally fulfilled, and the contrast was close to cloaca. On the 18th day all contrast was eliminated by the animal. Total time for contrast elimination was, in average, 17.6 ± 2.4 days, with the minimum of 12 and maximum of 22 days, with temperature at 27°C. The digestion of the food was slower in the duodenum, and faster in the colon-rectum, which presents lower indices of repletion.
Anatomia vascular das artérias renais e gonadais de Podocnemis unifilis Schweigger, 1812 (Testudines, Pelomedusidae - DOI: 10.4025/actascibiolsci.v31i2.680 Vascular anatomy of renal and gonadal arteries of Podocnemis unifilis Schweigger, 1812 (Testudines-Pelomedusidae - DOI: 10.4025/actascibiolsci.v31i2.680
Árthur Paulino Sanzo Kaminishi
Full Text Available Este trabalho foi desenvolvido com o intuito de enriquecer o conhecimento sobre a morfologia vascular das artérias renais e gonadais de Podocnemis unifilis, facilitando o entendimento da fisiologia clínica e cirúrgica destes animais. Foram utilizados cinco exemplares machos de Podocnemis unifilis (tracajá, coletados segundo a Licença nº 066/2004-Ibama/RAM. A artéria carótida esquerda e a veia femoral direita foram canuladas e, pelas mesmas, foi introduzida solução fisiológica para lavagem do sistema vascular; em seguida, aplicou-se solução de Neoprene Látex “450” corada com pigmento específico (Globo S/A Tintas e Pigmentos. O material foi fixado em solução de Formol tamponado a 10% por um período mínimo de 96h. Uma abertura central e de formato quadrangular foi feita na metade caudal do plastrão e casco, de forma a expor os ramos da artéria aorta que irrigam os rins e as gônadas. Observou-se que as artérias renais se originam da face ventral da artéria aorta dorsal, em número de dois pares para cada rim e, em um único exemplar, elas originaram uma artéria renal direita e duas esquerdas. A artéria gonadal surgiu a partir da artéria renal, e apenas um par penetrou pela face dorsal de cada gônada.This work was developed with the aim of enriching knowledge on the vascular morphology of renal and gonadal arteries of Podocnemis unifilis, thus increasing the understanding of the clinical and surgical physiology of these animals. Five Podocnemis unifilis males were used, collected according to license no. 066/2004-Ibama/RAM. The left carotid artery and right femoral vein were cannulated, and a serum solution was introduced to remove obstructions from the vascular system. A solution of Neoprene Latex “450” dye was injected. The material was fixed in a solution of 10% formaldehyde for a period of 96 hours. Next, a square-shaped central opening was made in the caudal end of the plastron and bridge, exposing the branches of the aorta that irrigate the kidneys and gonads. It was observed that the renal arteries were originated from the ventral face of the dorsal aorta artery, in a number of two pairs per kidney and, in a single case, they originated one right and two left renal arteries. The gonadal artery was originated from the renal artery, and only one pair penetrated the dorsal face of each gonad.
Aksoy, Serap; Almeida-Val, Vera Maria F; Azevedo, V C R; Baucom, Regina; Bazaga, Pilar; Beheregaray, L B; Bennetzen, Jeffrey L; Brassaloti, Ricardo A; Burgess, Treena I; Caccone, Adagisa; Chang, Shu-Mei; Ciampi, A Y; Ciancaleoni, S; Clímaco, Gisele T; Clouet, Cécil; Coimbra, Maria R M; Coutinho, Luiz L; Dantas, Hozana L; De Vega, Clara; Echodu, Richard; Enyaru, John; Figueira, Antonio; Filho, Manoel A G; Foltz, Britnie; Fressigné, L; Gadomski, Mateusz; Gauthier, Nathalie; Herrera, Carlos M; Hyseni, Chaz; Jorge, Erika C; Kaczmarczyk, Dariusz; Knott, Emily; Kuester, Adam; Lima, Ana P S; Lima, Maíra A; Lima, Marcos P; Longo, Ana Luiza B; Lor, Grant; Maggioni, Rodrigo; Marques, Thiago S; Martins, Aline R; Matoso, Daniele A; Medrano, Mónica; Mendonça, M A C; Mettler, Raeann; Nascimento, Priscila Roberta M; Negri, V; Oliveira, Karine K C; Oliveira, L O; Ovcarenko, Irina; Paula-Silva, Maria N; Raggi, L; Sandoval-Castillo, J; Santos, Carlos Henrique Dos Anjos; Martin Schaefer, H; Segelbacher, Gernot; Seino, Miyuki M; Sistrom, Mark; Taole, Matsepo M; Teske, P R; Tsagkarakou, Anastasia; Verdade, Luciano M; Villela, Priscilla M S; Vinson, C C; Wingfield, Brenda D; Wingfield, Michael J
This article documents the addition of 153 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brassica oleracea, Brycon amazonicus, Dimorphandra wilsonii, Eupallasella percnurus, Helleborus foetidus, Ipomoea purpurea, Phrynops geoffroanus, Prochilodus argenteus, Pyura sp., Sylvia atricapilla, Teratosphaeria suttonii, Trialeurodes vaporariorum and Trypanosoma brucei. These loci were cross-tested on the following species: Dimorphandra coccicinea, Dimorphandra cuprea, Dimorphandra gardneriana, Dimorphandra jorgei, Dimorphandra macrostachya, Dimorphandra mollis, Dimorphandra parviflora and Dimorphandra pennigera.
Aislamiento e identificación de microorganismos entéricos en muestras ambientales y cloacales en Crocodylus intermedius y testudines de la Estación de Biología Tropical Roberto Franco en Villavicencio, Colombia
C. A. Moreno
Full Text Available Los microorganismos entéricos han sido frecuentemente reportados como patógenos enmamíferos, aves, peces, reptiles y humanos, a pesar de hacer parte de su flora normal intestinal.La Estación de Biología Tropical Roberto Franco (EBTRF, lidera el programade recuperación del Caimán Llanero (Crocodylus intermedius, que se encuentra en inminentepeligro de extinción; adicionalmente cuenta con una colección viva de Testudinesque comprende más de 20 especies. Con el fin de determinar la presencia de potencialesenteropatógenos en el hábitat de los ejemplares, se obtuvieron 129 muestras ambientalesy cloacales de las especies allí encontradas; se utilizó el medio de cultivo CHROMagarOrientaciónBD® para realizar los aislamientos y la identificación microbiológica. Los resultadosmuestran una mayor presentación de flora gram negativa predominando microorganismosde los géneros (28%, Klebsiella sp (26%, Salmonella sp.(6%, Proteus sp (3% y Citrobacter sp. (1% Sin embargo, microorganismos del géneroEnterococcus sp. (gram positivo, fueron hallados en un mayor porcentaje (31% en todaslas muestras sin importar el origen de las mismas. Conscientes del riesgo que implica elaislamiento de microorganismos entéricos que pueden presentar un carácter zoonótico,se dio inicio a la implementación de un manual de bioseguridad para la Estación con elfin disminuir el riesgo para la población humana y animal.
Shi, H.; Parham, James F.; Simison, W. Brian; Wang, J.; Gong, S.; Fu, B.
Ten new turtle taxa were described from pet trade specimens from China since the 1980s (see Fritz and Obst, 1998; Fritz and Obst, 1999; Parham et al., 2001 for a review). Specimens similar to one of these taxa, Cuora serrata Iverson and McCord, 1992 (originally Cuora galbinifrons serrata, elevated by Fritz and Obst, 1997), were shown to be hybrids of male Cuora mouhotii (Gray, 1862; formerly Pyxidea, but see Stuart and Parham, 2004) and females of Cuora galbinifrons Bourret, 1939 or Cuora bourreti Obst and Reimann, 1994 (Parham et al., 2001; and Stuart and Parham, 2004).
Socio-economic and spatial determinants of anthropogenic predation on Yellow-spotted River Turtle, Podocnemis unifilis (Testudines: Pelomedusidae, nests in the Brazilian Amazon: Implications for sustainable conservation and management
Full Text Available Human expansion has drastically affected wildlife species across Amazonian waterways and the continued increase in rural populations across Amazonia is likely to increase pressure on widely exploited chelonian species. The lack of information evaluating determinants of human consumption patterns limits the effective implementation of conservation strategies. Our objective was to determine the relative importance of social, economic, and spatial variables on human exploitation of Podocnemis unifilis Troschel, 1848 around a sustainable-use protected area in the eastern Brazilian Amazon. We conducted interviews with 51 riverine residents to evaluate attitudes towards and exploitation of P. unifilis. We used multimodel inference to evaluate 12 working hypothesis predicting social, economic, and spatial influences on the occurrence and intensity of P. unifilis egg consumption. None of the respondents reported catching and/or eating adult P. unifilis and none sold adults or eggs during the previous year. Although the majority (58.8% of respondents had eaten P. unifilis eggs during the previous year and the species was occasionally caught in fishing nets, P. unifilis was rarely cited as a liked or disliked species. Our information theoretic analysis showed that spatial hypotheses were the most strongly supported whereas social and economic hypotheses were only weakly supported in explaining if and how many P. unifilis eggs had been consumed by riverine residents during the previous year. Our findings suggest that current Amazonian development patterns may be associated with reduced consumption of adult P. unifilis, but consumption of eggs together with other indirect anthropogenic perturbations continue to threaten remaining P. unifilis populations.
How nest translocation-time, clutch size and presence of yolkless eggs affected hatching success in Dermochelys coriacea (Linnaeus, 1766 (Testudines: Dermochelyidae, at Projeto Tamar-Ibama, Espirito Santo, Brazil
Dal Pont Morisso, Eduardo
Full Text Available En este estudio se analizó el manejo de los nidos de la especie Dermochelys coriacea, utilizados por el Proyecto Tamar-Ibama entre las temporadas reproductivas de 1989/1990 a 1998/1999, para verificación del éxito de la eclosión en relación a los tiempos de traslación. El estudio se realizó en el litoral norte del Estado de Espírito Santo. Existió tendencia a que el tiempo de traslación influencie el éxito de eclosión de los nidos. Los nidos trasladados entre 6 y más de 24 horas presentaron mayor cantidad de huevos sin desarrollo embrionario. No se encontró relación entre el número de huevos inviables trasladados y el tamaño de la postura, con el porcentual de eclosión. Se sugiere que la traslación sea realizada hasta las 6 horas, o 15 días después de la oviposición. The results of managing Dermochelys coriacea (Linnaeus, 1766 nests by Projeto TAMAR - IBAMA, in Northern Espírito Santo, Brazil, during the nesting seasons from 1989/90 to 1998/99 are analyzed. The influence of the translocation time on hatching success of the studied nests is discussed. The time translocation, in relation to natural oviposition, seems to increase the number of non-developed eggs found in each nest if translocated between 6 hours and 15 days post egg-laying. There was no relationship established between either the number of yolkless eggs in a translocated nest or the clutch size, with hatching success. The translocation of Dermochelys nests either within 6 hours or after 15 days from natural oviposition is recommended.
Full Text Available We studied turtles in the focal area of the Reserva de Desenvolvimento Sustentável Mamirauá from September 1996 through August 1998. The reserve is located in the western part of the Brazilian Amazon, between the Japurá, Solimões, and Auti-Paraná rivers, near the city of Tefé, in the state of Amazonas. In this study the population structure, sex ratio and abundance of Podocnemis sextuberculata were investigated. We intensively studied the population of turtles in the Jarauá river basin in order to determine population structure. We sampled turtles in different sectors in the focal area of the reserve for one month each year during July and August of 1997 and 1998. We captured the turtles using flag gill nets and trammel nets. The type of net and the size of the nets used influenced the size of the turtles captured. The nets used by the local fishermen were sexually selective in the capture of the turtles. Sevent-two percent of the P. sextuberculata captured were adults; 13% juveniles and 14.7% subadults. During the second year of the study we found a lower density of turtles in the five areas wich we sampled each years. The sex ratio of captured P. sextuberculata was 1.87 males per female.
Full Text Available We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4, in Islas Del Rosario (Bolívar; n=2 in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI, we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The ethodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity.
Aislamiento e identificación de microorganismos entéricos en muestras ambientales y cloacales en Crocodylus intermedius y testudines de la Estación de Biología Tropical Roberto Franco en Villavicencio, Colombia
C. A. Moreno; A. P. Pulido; D. A. Pachón
Los microorganismos entéricos han sido frecuentemente reportados como patógenos enmamíferos, aves, peces, reptiles y humanos, a pesar de hacer parte de su flora normal intestinal.La Estación de Biología Tropical Roberto Franco (EBTRF), lidera el programade recuperación del Caimán Llanero (Crocodylus intermedius), que se encuentra en inminentepeligro de extinción; adicionalmente cuenta con una colección viva de Testudinesque comprende más de 20 especies. Con el fin de determinar la presencia d...
Incubação artificial dos ovos e processo de eclosão em Trachemys dorbignyi (Duméril & Bibron (Reptilia, Testudines, Emydidae Artificial egg incubation and hatching proccess in Trachemys dorbignyi (Duméril & Bibron (Reptilia, Testudimes, Emydidae
Flavio de Barros Molina
Full Text Available Artificial incubation of 558 eggs from 70 clutches of Trachemys dorbignyi (Duméril & Bibron, 1835 were performed at São Paulo Zoo during 1992 and 1993. Hatching occurred when eggs were incubated between 25 and 31.5oC. Incubation time varied from 54 (at 31.5oC to 120 days (at 25oC, similarly to Trachemys scripla sspp. Hatchling used the caruncle to made small incisions in the egg shell, latter enlarged by movements of the head and forefeet. Hatching usually lasted from one to two days. Newborn's carapace and plastron showed their natural form few hours after the emergence from the egg shell. During the third or fourth week, caruncle usually disappeared, and yolk sac was completely absorbed. Average (x±sd measures of newborn were 3.55±0.18cm of carapace length, 3.35±0.17cm of plastron length, and 10.73±1.36g of weight.
Nicholson, David B.; Holroyd, Patricia A.; Valdes, Paul; Barrett, Paul M.
The latitudinal biodiversity gradient (LBG)-the pattern of increasing taxonomic richness with decreasing latitude-is prevalent in the structure of the modern biota. However, some freshwater taxa show peak richness at mid-latitudes; for example, extant Testudines (turtles, terrapins and tortoises) exhibit their greatest diversity at 25° N, a pattern sometimes attributed to recent bursts of climatically mediated species diversification. Here, we test whether this pattern also characterizes the Mesozoic distribution of turtles, to determine whether it was established during either their initial diversification or as a more modern phenomenon. Using global occurrence data for non-marine testudinate genera, we find that subsampled richness peaks at palaeolatitudes of 15-30° N in the Jurassic, 30-45° N through the Cretaceous to the Campanian, and from 30° to 60° N in the Maastrichtian. The absence of a significant diversity peak in southern latitudes is consistent with results from climatic models and turtle niche modelling that demonstrate a dearth of suitable turtle habitat in Gondwana during the Jurassic and Late Cretaceous. Our analyses confirm that the modern testudinate LBG has a deep-time origin and further demonstrate that LBGs are not always expressed as a smooth, equator-to-pole distribution.
Cadena, Edwin A.; Anaya, Federico; Croft, Darin A.
We describe the first Miocene turtle remains from Bolivia, which were collected from the late middle Miocene (13.18-13.03 Ma) of Quebrada Honda, southern Bolivia. This material includes a large scapula-acromion and fragmentary shell elements conferred to the genus Chelonoidis (Testudinidae), and a left xiphiplastron from a pleurodire or side-necked turtle, conferred to Acanthochelys (Chelidae). The occurrence of a giant tortoise and a freshwater turtle suggests that the paleoelevation of the region when the fossils were deposited was lower than has been estimated by stable isotope proxies, with a maximum elevation probably less than 1000 m. At a greater elevation, cool temperatures would have been beyond the tolerable physiological limits for these turtles and other giant ectotherm reptiles.
AMAVET Patricia Susana
The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii (Chelidae) and Trachemys dorbigni (Emydidae), two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the ampliifed products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work.
Yuri C. C. Lima
Full Text Available The Herpetofauna of an urban fragment of Atlantic Forest was investigated in relation to species richness and habitat use. Fourteen species of amphibian anurans pertaining to the families Bufonidae, Brachycephalidae, Hylidae, Leptodactylidae, Leiuperidae, Microhylidae and Ranidae were recorded. The reptiles were represented by 37 species, distributed in the families Gekkonidae, Gymnophthalmidae, Polychrotidae, Scincidae, Teiidae, Tropiduridae, Amphisbaenidae, Boidae, Colubridae, Elapidae, Typhlopidae, Chelidae, Testudinidae and Alligatoridae. Most of the recorded species presented wide geographic distribution, although some of them had distributions that were restricted to the Atlantic Forest. The species richness of Mata do Buraquinho is relatively high for an urban fragment of Atlantic Forest, and the observed anthropogenic impacts show the urgent necessity of conservation in order to guarantee the viability of populations of amphibians and reptiles.
Olivieri, D N; von Haeften, B; Sánchez-Espinel, C; Faro, J; Gambón-Deza, F
Reptiles and mammals diverged over 300 million years ago, creating two parallel evolutionary lineages amongst terrestrial vertebrates. In reptiles, two main evolutionary lines emerged: one gave rise to Squamata, while the other gave rise to Testudines, Crocodylia, and Aves. In this study, we determined the genomic variable (V) exons from whole genome shotgun sequencing (WGS) data in reptiles corresponding to the three main immunoglobulin (IG) loci and the four main T cell receptor (TR) loci. We show that Squamata lack the TRG and TRD genes, and snakes lack the IGKV genes. In representative species of Testudines and Crocodylia, the seven major IG and TR loci are maintained. As in mammals, genes of the IG loci can be grouped into well-defined IMGT clans through a multi-species phylogenetic analysis. We show that the reptilian IGHV and IGLV genes are distributed amongst the established mammalian clans, while their IGKV genes are found within a single clan, nearly exclusive from the mammalian sequences. The reptilian and mammalian TRAV genes cluster into six common evolutionary clades (since IMGT clans have not been defined for TR). In contrast, the reptilian TRBV genes cluster into three clades, which have few mammalian members. In this locus, the V exon sequences from mammals appear to have undergone different evolutionary diversification processes that occurred outside these shared reptilian clans. These sequences can be obtained in a freely available public repository (http://vgenerepertoire.org).
Full Text Available Se llevaron a cabo estudios adicionales sobre problemas de reproducción de la babilla (Caiman sclerops y tres especies de tortugas (Podocnemis expansa, Podocnemis unifilis y Phrynops geoffroanus tuberosus. Los resultados principales son los siguientes: 1 Caiman sclerops. Los huevos miden de 67.0 : 41.5 mm. a 73.5 : 41.0 mm. La longitud total de los recién nacidos comprende de 239.5 mm. a 255.5 mm.; pesan entre 32 g, 250 mg. y 47 g. 2 Podocnemis expansa. No es nativa del Alto Caquetá, sino ha sido introducida de su curso bajo y del Putumayo en 1940, 1943 y 1955. Actualmente está establecida y se reproduce. Los huevos miden de 44.5 : 36.5 mm, a 48.5 : 45.5 mm., y pesan de 34 g, 350 mg. a 44 g, 300 mg. La Iongitud del Carapax de los recién nacidos varía entre 39.5 mm. y 45.0 mm.; pesan de 17 g, 300 mg. a 20 g. 3 Podocnemis unifilis. Los huevos miden de 41.0 : 28.5 mm. a 51.0 33.5 mm., y pesan entre 15 g. y 31 g.; los recién nacidos de 34.0 a 48.0 mm., pesan de 8 g, 750 mg. a 21 g, 100 mg. Evidentemente, la luz constituye el factor más decisivo en relación con la orientación de la cría recién salida del nido, pero indudablemente existen también otros factores todavía desconocidos. Desarrollan una velocidad considerable para llegar al agua, así, por ejemplo, necesitan unos 25 minutos para recorrer una distancia de 96 metros; no permanecen en las aguas poco profundas cerca de la orilla, sino buscan en seguida las partes hondas donde encuentran mejor protección. En cambio, las babillas recién salidas si permanecen cerca de las orillas, ya que están protegidas por la hembra. 4 Phrynops geoffroanus tuberosus. Los huevos miden entre 32.5 31.5 mm, y 35.0 : 34.0 mm.; pesan de 13 g, 550 mg. a 22 g.; los recién nacidos entre 38.5 mm, y 48.0 mm., .y pesan de 7 g, 700 mg. a 14 g, 750 mg. 5 La Matamata (Chelus fimbriatus es, en realidad, nativa en el Alto Caquetá, y no ocasionalmente traída de otros ríos, como se
Thales de Lema
Full Text Available This paper reports the preliminary results of two years of herpetogeographic studies in the region called «Grande Porto Alegre» at its northern portion. The physiognomy shows, at North, slopes of the Brazilian Southern Plateau, more or less forested in a general view; these elevations graãuálly decrease to the South until the levei of the sea to Atlantic littoral by the eastern border, and to Rio Grande do Sul savanah, which extends to Uruguay and Argentina. The biota is transitional from the highland prairies and forests of the plateau to lowland prairies of the pampas. Three faunistic Provinces are included: Guarani, Pampean, and Tupi. The most conspicuous dispersion is shown by the taxa proceeding from the plateau to savanah (Dispersion Centre Guarani; the pampean species occur in low percentage (Dispersion Centre Uruguay, and an inappreciable account of Tupi fauna (Dispersion Centre of «Serra do Mar». The new records are: presence of Chironius pyrrhopogon, Hydrodynastes gigas, Bothrops neuwiedi para-naensis, Micrurus corallinus; intergradation zone of Guarani and Pampean geographic roces; and new taxa of snakes, which will be described brieflly. The systematic composition presents: Chelonia, 4 (3 Chelidae, 1 Testudinidae; Sauria, 10 (1 Gekkonidae, 2 Iguanidae, 1 Anguidae, 3 Teiidae, 3 Amphisbaenidae; Serpentes, 45 (35 Colubridae, 4 Elapidae, 6 Viperidae; Crocodylia (1 Crocodylidae.
Meredith Robert W
Full Text Available Abstract Background Secondary edentulism (toothlessness has evolved on multiple occasions in amniotes including several mammalian lineages (pangolins, anteaters, baleen whales, birds, and turtles. All edentulous amniote clades have evolved from ancestors with enamel-capped teeth. Previous studies have documented the molecular decay of tooth-specific genes in edentulous mammals, all of which lost their teeth in the Cenozoic, and birds, which lost their teeth in the Cretaceous. By contrast with mammals and birds, tooth loss in turtles occurred in the Jurassic (201.6-145.5 Ma, providing an extended time window for tooth gene degradation in this clade. The release of the painted turtle and Chinese softshell turtle genomes provides an opportunity to recover the decayed remains of tooth-specific genes in Testudines. Results We queried available genomes of Testudines (Chrysemys picta [painted turtle], Pelodiscus sinensis [Chinese softshell turtle], Aves (Anas platyrhynchos [duck], Gallus gallus [chicken], Meleagris gallopavo [turkey], Melopsittacus undulatus [budgerigar], Taeniopygia guttata [zebra finch], and enamelless mammals (Orycteropus afer [aardvark], Choloepus hoffmanni [Hoffmann’s two-toed sloth], Dasypus novemcinctus [nine-banded armadillo] for remnants of three enamel matrix protein (EMP genes with putative enamel-specific functions. Remnants of the AMBN and ENAM genes were recovered in Chrysemys and retain their original synteny. Remnants of AMEL were recovered in both testudines, although there are no shared frameshifts. We also show that there are inactivated copies of AMBN, AMEL and ENAM in representatives of divergent avian lineages including Galloanserae, Passeriformes, and Psittaciformes, and that there are shared frameshift mutations in all three genes that predate the basal split in Neognathae. Among enamelless mammals, all three EMP genes exhibit inactivating mutations in Orycteropus and Choloepus. Conclusions Our results
Moura, Geraldo Jorge Barbosa; Albuquerque, Ulysses Paulino
There have been very few ethnopharmacological studies performed on the traditional use of fossil species, although a few records have been conducted in Asia, Africa, and Europe. This study is the first ever to be performed on the use of Testudine (turtle) fossils for folk medicine in Latin America. An investigation was conducted in the Araripe Basin, which is one of the most important fossil-bearing reserves in the world due to the diversity, endemism, and quality of preservation of its fossils. We propose the formalization of a new discipline called ethnopaleontology, which will involve the study of the dynamic relationship between humans and fossils, from human perception to direct use. PMID:21969843
Vaz-Silva, W; Oliveira, R M; Gonzaga, A F N; Pinto, K C; Poli, F C; Bilce, T M; Penhacek, M; Wronski, L; Martins, J X; Junqueira, T G; Cesca, L C C; Guimarães, V Y; Pinheiro, R D
The region of Volta Grande do Xingu River, in the state of Pará, presents several kinds of land use ranging from extensive cattle farming to agroforestry, and deforestation. Currently, the Belo Monte Hydroelectric Power Plant affects the region. We present a checklist of amphibians and reptiles of the region and discuss information regarding the spatial distribution of the assemblies based on results of Environmental Programmes conducted in the area. We listed 109 amphibian (Anura, Caudata, and Gymnophiona) and 150 reptile (Squamata, Testudines, and Crocodylia) species. The regional species richness is still considered underestimated, considering the taxonomic uncertainty, complexity and cryptic diversity of various species, as observed in other regions of the Amazon biome. Efforts for scientific collection and studies related to integrative taxonomy are needed to elucidate uncertainties and increase levels of knowledge of the local diversity.
Full Text Available Abstract The region of Volta Grande do Xingu River, in the state of Pará, presents several kinds of land use ranging from extensive cattle farming to agroforestry, and deforestation. Currently, the Belo Monte Hydroelectric Power Plant affects the region. We present a checklist of amphibians and reptiles of the region and discuss information regarding the spatial distribution of the assemblies based on results of Environmental Programmes conducted in the area. We listed 109 amphibian (Anura, Caudata, and Gymnophiona and 150 reptile (Squamata, Testudines, and Crocodylia species. The regional species richness is still considered underestimated, considering the taxonomic uncertainty, complexity and cryptic diversity of various species, as observed in other regions of the Amazon biome. Efforts for scientific collection and studies related to integrative taxonomy are needed to elucidate uncertainties and increase levels of knowledge of the local diversity.
Klein, Wilfried; Codd, Jonathan R
Using specialized respiratory structures such as gills, lungs and or a tracheal system, animals take up oxygen and release carbon dioxide. The efficiency of gas exchange, however, may be constrained by the morphology of the respiratory organ itself as well as by other aspects of an animal's physiology such as feeding, circulation or locomotion. Herein we discuss some aspects of the functional link between the respiratory and locomotor systems, such as gill morphology of sharks as a factor limiting maximum aerobic scope, respiratory constraints among legless lizards, lung morphology of testudines, trade-offs between locomotion and respiration among birds, reconstruction of the respiratory system of sauropods, respiration of mice during locomotion as well as some aspects of gas exchange among insects. Data covering such a broad spectrum of interactions between the locomotor and respiratory systems shall allow us to place breathing and locomotion into a wider context of evolution of oxygen.
Geraldo Jorge Barbosa Moura
Full Text Available There have been very few ethnopharmacological studies performed on the traditional use of fossil species, although a few records have been conducted in Asia, Africa, and Europe. This study is the first ever to be performed on the use of Testudine (turtle fossils for folk medicine in Latin America. An investigation was conducted in the Araripe Basin, which is one of the most important fossil-bearing reserves in the world due to the diversity, endemism, and quality of preservation of its fossils. We propose the formalization of a new discipline called ethnopaleontology, which will involve the study of the dynamic relationship between humans and fossils, from human perception to direct use.
Alfaro Nuñez, Luis Alonso
This thesis describes various aspects of marine turtle (Testudines) evolution and tackles a well-described and controversial disease of these animals, fibropapillomatosis (FP), which is believed to be caused by the Chelonid fibropapilloma-associated herpesvirus (CFPHV). A large dataset of samples...... from five globally distributed marine turtle species was analysed in order to detect the presence of CFPHV DNA. These samples represent both clinical (FP tumour) and subclinical (non-tumour) CFPHV infections. Validated methods were developed and applied in order to determine the global prevalence...... of the CFPHV among these species. Singleplex and nested PCR, Sanger sequencing and qPCR techniques were used to achieve these goal. Moreover, by using next generation sequencing we obtained a large number of full turtle mitochondrial genomes and a few entire CFPHV genomes were obtained from various samples...
Alfaro Nuñez, Luis Alonso
This thesis describes various aspects of marine turtle (Testudines) evolution and tackles a well-described and controversial disease of these animals, fibropapillomatosis (FP), which is believed to be caused by the Chelonid fibropapilloma-associated herpesvirus (CFPHV). A large dataset of samples...... from five globally distributed marine turtle species was analysed in order to detect the presence of CFPHV DNA. These samples represent both clinical (FP tumour) and subclinical (non-tumour) CFPHV infections. Validated methods were developed and applied in order to determine the global prevalence...... of the CFPHV among these species. Singleplex and nested PCR, Sanger sequencing and qPCR techniques were used to achieve these goal. Moreover, by using next generation sequencing we obtained a large number of full turtle mitochondrial genomes and a few entire CFPHV genomes were obtained from various samples...
Kawagoshi, Taiki; Uno, Yoshinobu; Nishida, Chizuko; Matsuda, Yoichi
Reptiles have a wide diversity of sex-determining mechanisms and types of sex chromosomes. Turtles exhibit temperature-dependent sex determination and genotypic sex determination, with male heterogametic (XX/XY) and female heterogametic (ZZ/ZW) sex chromosomes. Identification of sex chromosomes in many turtle species and their comparative genomic analysis are of great significance to understand the evolutionary processes of sex determination and sex chromosome differentiation in Testudines. The Mexican giant musk turtle (Staurotypus triporcatus, Kinosternidae, Testudines) and the giant musk turtle (Staurotypus salvinii) have heteromorphic XY sex chromosomes with a low degree of morphological differentiation; however, their origin and linkage group are still unknown. Cross-species chromosome painting with chromosome-specific DNA from Chinese soft-shelled turtle (Pelodiscus sinensis) revealed that the X and Y chromosomes of S. triporcatus have homology with P. sinensis chromosome 6, which corresponds to the chicken Z chromosome. We cloned cDNA fragments of S. triporcatus homologs of 16 chicken Z-linked genes and mapped them to S. triporcatus and S. salvinii chromosomes using fluorescence in situ hybridization. Sixteen genes were localized to the X and Y long arms in the same order in both species. The orders were also almost the same as those of the ostrich (Struthio camelus) Z chromosome, which retains the primitive state of the avian ancestral Z chromosome. These results strongly suggest that the X and Y chromosomes of Staurotypus turtles are at a very early stage of sex chromosome differentiation, and that these chromosomes and the avian ZW chromosomes share the same origin. Nonetheless, the turtles and birds acquired different systems of heterogametic sex determination during their evolution.
Full Text Available Reptiles have a wide diversity of sex-determining mechanisms and types of sex chromosomes. Turtles exhibit temperature-dependent sex determination and genotypic sex determination, with male heterogametic (XX/XY and female heterogametic (ZZ/ZW sex chromosomes. Identification of sex chromosomes in many turtle species and their comparative genomic analysis are of great significance to understand the evolutionary processes of sex determination and sex chromosome differentiation in Testudines. The Mexican giant musk turtle (Staurotypus triporcatus, Kinosternidae, Testudines and the giant musk turtle (Staurotypus salvinii have heteromorphic XY sex chromosomes with a low degree of morphological differentiation; however, their origin and linkage group are still unknown. Cross-species chromosome painting with chromosome-specific DNA from Chinese soft-shelled turtle (Pelodiscus sinensis revealed that the X and Y chromosomes of S. triporcatus have homology with P. sinensis chromosome 6, which corresponds to the chicken Z chromosome. We cloned cDNA fragments of S. triporcatus homologs of 16 chicken Z-linked genes and mapped them to S. triporcatus and S. salvinii chromosomes using fluorescence in situ hybridization. Sixteen genes were localized to the X and Y long arms in the same order in both species. The orders were also almost the same as those of the ostrich (Struthio camelus Z chromosome, which retains the primitive state of the avian ancestral Z chromosome. These results strongly suggest that the X and Y chromosomes of Staurotypus turtles are at a very early stage of sex chromosome differentiation, and that these chromosomes and the avian ZW chromosomes share the same origin. Nonetheless, the turtles and birds acquired different systems of heterogametic sex determination during their evolution.
Full Text Available Abstract Background The Recombination Activating Proteins, RAG1 and RAG2, play a crucial role in the immune response in vertebrates. Among the nuclear markers currently used for phylogenetic purposes, Rag1 has especially enjoyed enormous popularity, since it successfully contributed to elucidating the relationships among and within a large variety of vertebrate lineages. We here report on a comparative investigation of the genetic variation, base composition, presence of indels, and selection in Rag1 in different vertebrate lineages (Actinopterygii, Amphibia, Aves, Chondrichthyes, Crocodylia, Lepidosauria, Mammalia, and Testudines through the analysis of 582 sequences obtained from Genbank. We also analyze possible differences between distinct parts of the gene with different type of protein functions. Results In the vertebrate lineages studied, Rag1 is over 3 kb long. We observed a high level of heterogeneity in base composition at the 3rd codon position in some of the studied vertebrate lineages and in some specific taxa. This result is also paralleled by taxonomic differences in the GC content at the same codon position. Moreover, positive selection occurs at some sites in Aves, Lepidosauria and Testudines. Indels, which are often used as phylogenetic characters, are more informative across vertebrates in the 5' than in the 3'-end of the gene. When the entire gene is considered, the use of indels as phylogenetic character only recovers one major vertebrate clade, the Actinopterygii. However, in numerous cases insertions or deletions are specific to a monophyletic group. Conclusions Rag1 is a phylogenetic marker of undoubted quality. Our study points to the need of carrying out a preliminary investigation on the base composition and the possible existence of sites under selection of this gene within the groups studied to avoid misleading resolution. The gene shows highly heterogeneous base composition, which affects some taxa in particular and
Rice, Ritva; Kallonen, Aki; Cebra-Thomas, Judith; Gilbert, Scott F
The dorsal and ventral aspects of the turtle shell, the carapace and the plastron, are developmentally different entities. The carapace contains axial endochondral skeletal elements and exoskeletal dermal bones. The exoskeletal plastron is found in all extant and extinct species of crown turtles found to date and is synaptomorphic of the order Testudines. However, paleontological reconstructed transition forms lack a fully developed carapace and show a progression of bony elements ancestral to the plastron. To understand the evolutionary development of the plastron, it is essential to know how it has formed. Here we studied the molecular development and patterning of plastron bones in a cryptodire turtle Trachemys scripta We show that plastron development begins at developmental stage 15 when osteochondrogenic mesenchyme forms condensates for each plastron bone at the lateral edges of the ventral mesenchyme. These condensations commit to an osteogenic identity and suppress chondrogenesis. Their development overlaps with that of sternal cartilage development in chicks and mice. Thus, we suggest that in turtles, the sternal morphogenesis is prevented in the ventral mesenchyme by the concomitant induction of osteogenesis and the suppression of chondrogenesis. The osteogenic subroutines later direct the growth and patterning of plastron bones in an autonomous manner. The initiation of plastron bone development coincides with that of carapacial ridge formation, suggesting that the development of dorsal and ventral shells are coordinated from the start and that adopting an osteogenesis-inducing and chondrogenesis-suppressing cell fate in the ventral mesenchyme has permitted turtles to develop their order-specific ventral morphology.
Rodrigues, João Fabrício Mota; Diniz-Filho, José Alexandre Felizola
Habitat may be viewed as an important life history component potentially related to diversification patterns. However, differences in diversification rates between aquatic and terrestrial realms are still poorly explored. Testudines is a group distributed worldwide that lives in aquatic and terrestrial environments, but until now no-one has evaluated the diversification history of the group as a whole. We aim here to investigate the diversification history of turtles and to test if habitat influenced speciation rate in these animals. We reconstructed the phylogeny of the modern species of chelonians and estimated node divergence dates using molecular markers and a Bayesian approach. Then, we used Bayesian Analyses of Macroevolutionary Mixtures to evaluate the diversification history of turtles and evaluate the effect of habitat on this pattern. Our reconstructed phylogeny covered 300 species (87% of the total diversity of the group). We found that the emydid subfamily Deirochelyinae, which forms the turtle hotspot in south-eastern United States, had an increase in its speciation rate, and that Galapagos tortoises had similar increases. Current speciation rates are lower in terrestrial turtles, contradicting studies supporting the idea terrestrial animals diversify more than aquatic species. Our results suggest that habitat, ecological opportunities, island invasions, and climatic factors are important drivers of diversification in modern turtles and reinforce the importance of habitat as a diversification driver. Copyright © 2016 Elsevier Inc. All rights reserved.
Sánchez Hemández, B.
Full Text Available Sediments of Tithonian-Barremian of Iberian Basin in the surroundings of Galve (Teniel, Spain have a high content of vertebrate's remains. These ones have been studied since s. XX until today by different scientists. More than ninety taxons have been mentioned in these papers, distributed in Hybodontiformes, Squalomorpha, Batoidea, Rajiforms, Amphibia, Reptilia (Chelonia, Sauria, Crocodylia, Pterosauria, Ornithischia, Saurischia and Marnmalia.Los sedimentos del Tithónico-Barremiense de la Cuenca Ibérica aflorantes en los alrededores de la localidad de Galve (Teniel, son particularmente ricos en restos de vertebrados mesozoicos. Estos han sido estudiados por diferentes autores, desde principios del siglo xx hasta la actualidad. El objetivo del presente artículo es recopilar los distintos taxones que han sido citados para esta área, a lo largo del tiempo, recogiéndose más de noventa taxones distribuidos entre Hybodontiformes, Squalomorpha, Batoidea, Rajiformes, Amphibia, Reptilia (Testudines, Sauria, Crocodilia, Pterosauria, Ornithischia, Saurischia y Mammalia.
de Freitas, Marco Antonio; Vieira, Ruhan Saldanha; Entiauspe-Neto, Omar Machado; Sousa, Samantha Oliveira e; Farias, Tayse; Sousa, Alanna Grazieli; de Moura, Geraldo Jorge Barbosa
Abstract Understanding the biodiversity of an area is the first step for establishing effective interventions for conservation, especially when it comes to herpetofauna, since 4.1% and 9.2%, respectively, of Brazilian amphibians and reptiles are endangered. The aim of this study is to identify the composition of the herpetofauna occurring in the Northwest Amazonian state of Maranhão, with a focus on the Gurupi Biological Reserve and surrounding areas. Samples were collected between May 2012 and October 2013 (18 months), through pitfall traps, time constrained active search, and opportunistic encounters, and these records were supplemented by specimens collected by third parties and by bibliographic records. A total of 131 species were recorded: 31 species of amphibians and 100 species of reptiles (six testudines, 30 lizards, two amphisbaenas, 60 snakes and two alligators), including some species new to the state of Maranhão and the northeast region of Brazil. This inventory contributes to the knowledge of the herpetofauna for the Belém Endemism Center, the most devastated region of the Brazilian Amazon, and considered poorly sampled. PMID:28144181
Brilhante, Raimunda Sâmia Nogueira; Rodrigues, Pedro Henrique de Aragão; de Alencar, Lucas Pereira; Riello, Giovanna Barbosa; Ribeiro, Joyce Fonteles; de Oliveira, Jonathas Sales; Castelo-Branco, Débora de Souza Collares Maia; Bandeira, Tereza de Jesus Pinheiro Gomes; Monteiro, André Jalles; Rocha, Marcos Fábio Gadelha; Cordeiro, Rossana de Aguiar; Moreira, José Luciano Bezerra; Sidrim, José Júlio Costa
The aim of this study was to evaluate the antifungal susceptibility of Candida spp. recovered from tortoises (Chelonoidis spp.) and sea turtles (Chelonia mydas, Caretta caretta, Lepidochelys olivacea, Eretmochelys imbricata). For this purpose, material from the oral cavity and cloaca of 77 animals (60 tortoises and 17 sea turtles) was collected. The collected specimens were seeded on 2% Sabouraud dextrose agar with chloramphenicol, and the identification was carried out by morphological and biochemical methods. Sixty-six isolates were recovered from tortoises, out of which 27 were C. tropicalis, 27 C. famata, 7 C. albicans, 4 C. guilliermondii and 1 C. intermedia, whereas 12 strains were obtained from sea turtles, which were identified as Candida parapsilosis (n = 4), Candida guilliermondii (n = 4), Candida tropicalis (n = 2), Candida albicans (n = 1) and Candida intermedia (n = 1). The minimum inhibitory concentrations for amphotericin B, itraconazole and fluconazole ranged from 0.03125 to 0.5, 0.03125 to >16 and 0.125 to >64, respectively. Overall, 19 azole-resistant strains (14 C. tropicalis and 5 C. albicans) were found. Thus, this study shows that Testudines carry azole-resistant Candida spp.
Joyce, Walter G; Schoch, Rainer R; Lyson, Tyler R
Proterochersis robusta from the Late Triassic (Middle Norian) of Germany is the oldest known fossil turtle (i.e. amniote with a fully formed turtle shell), but little is known about its anatomy. A newly prepared, historic specimen provides novel insights into the morphology of the girdles and vertebral column of this taxon and the opportunity to reassess its phylogenetic position. The anatomy of the pectoral girdle of P. robusta is similar to that of other primitive turtles, including the Late Triassic (Carnian) Proganochelys quenstedti, in having a vertically oriented scapula, a large coracoid foramen, a short acromion process, and bony ridges that connect the acromion process with the dorsal process, glenoid, and coracoid, and by being able to rotate along a vertical axis. The pelvic elements are expanded distally and suturally attached to the shell, but in contrast to modern pleurodiran turtles the pelvis is associated with the sacral ribs. The primary homology of the character "sutured pelvis" is unproblematic between P. robusta and extant pleurodires. However, integration of all new observations into the most complete phylogenetic analysis that support the pleurodiran nature of P. robusta reveals that this taxon is more parsimoniously placed along the phylogenetic stem of crown Testudines. All current phylogenetic hypotheses therefore support the basal placement of this taxon, imply that the sutured pelvis of this taxon developed independently from that of pleurodires, and conclude that the age of the turtle crown is Middle Jurassic.
Angielczyk, Kenneth D; Burroughs, Robert W; Feldman, Chris R
Understanding how and why biodiversity is structured across the globe has been central to ecology, evolution, and biogeography even before those disciplines took their modern forms. Three global-scale patterns in particular have been the focus of research and debate for decades: latitudinal gradients in species richness (richness decreases with increasing latitude), body size (body size increases with increasing latitude in endotherms; Bergmann's rule), and geographic range size (range size increases with increasing latitude; Rapoport's rule). Despite decades of study, the generality and robustness of these trends have been debated, as have their underlying causes. Here we investigate latitudinal gradients in species richness, body size, and range size in the world's turtles (Testudines), and add more evidence that these rules do not seem to apply across all taxa. We show that turtle diversity actually peaks at 25° north, a highly unusual global pattern. Turtles also fail to follow Bergmann's Rule, and may show the converse (larger at lower latitudes), though trends are weak. Turtles also show a complex relationship between latitude and range size that does not directly follow Rapoport's rule. Body size and geographic range size are significantly correlated, and multiple abiotic and biotic variables help explain the relationships between latitude and species diversity, body size, and range size. Although we show that turtles do not strictly follow some classic biogeographical rules, we also call for further in-depth research to investigate potential causal mechanisms for these atypical patterns.
Lee, M S Y
Adding new taxa to morphological phylogenetic analyses without substantially revising the set of included characters is a common practice, with drawbacks (undersampling of relevant characters) and potential benefits (character selection is not biased by preconceptions over the affinities of the 'retrofitted' taxon). Retrofitting turtles (Testudines) and other taxa to recent reptile phylogenies consistently places turtles with anapsid-grade parareptiles (especially Eunotosaurus and/or pareiasauromorphs), under both Bayesian and parsimony analyses. This morphological evidence for turtle-parareptile affinities appears to contradict the robust genomic evidence that extant (living) turtles are nested within diapsids as sister to extant archosaurs (birds and crocodilians). However, the morphological data are almost equally consistent with a turtle-archosaur clade: enforcing this molecular scaffold onto the morphological data does not greatly increase tree length (parsimony) or reduce likelihood (Bayesian inference). Moreover, under certain analytic conditions, Eunotosaurus groups with turtles and thus also falls within the turtle-archosaur clade. This result raises the possibility that turtles could simultaneously be most closely related to a taxon traditionally considered a parareptile (Eunotosaurus) and still have archosaurs as their closest extant sister group.
All derived turtles are characterized by one of the strongest reductions of the dorsal elements among Amniota, and have only 10 dorsal and eight cervical vertebrae. I demonstrate that the Late Triassic turtles, which represent successive stages of the shell evolution, indicate that the shift of the boundary between the cervical and dorsal sections of the vertebral column occurred over the course of several million years after the formation of complete carapace. The more generalized reptilian formula of at most seven cervicals and at least 11 dorsals is thus plesiomorphic for Testudinata. The morphological modifications associated with an anterior homeotic change of the first dorsal vertebra towards the last cervical vertebra in the Triassic turtles are partially recapitulated by the reduction of the first dorsal vertebra in crown-group Testudines, and they resemble the morphologies observed under laboratory conditions resulting from the experimental changes of Hox gene expression patterns. This homeotic shift hypothesis is supported by the, unique to turtles, restriction of Hox-5 expression domains, somitic precursors of scapula, and brachial plexus branches to the cervical region, by the number of the marginal scute-forming placodes, which was larger in the Triassic than in modern turtles, and by phylogenetic analyses.
Edwards, Taylor; Karl, Alice E.; Vaughn, Mercy; Rosen, Philip C.; Torres, Cristina Meléndez; Murphy, Robert W.
Abstract Desert tortoises (Testudines; Testudinidae; Gopherus agassizii group) have an extensive distribution throughout the Mojave, Colorado, and Sonoran desert regions. Not surprisingly, they exhibit a tremendous amount of ecological, behavioral, morphological and genetic variation. Gopherus agassizii was considered a single species for almost 150 years but recently the species was split into the nominate form and Morafka’s desert tortoise, Gopherus morafkai, the latter occurring south and east of the Colorado River. Whereas a large body of literature focuses on tortoises in the United States, a dearth of investigations exists for Mexican animals. Notwithstanding, Mexican populations of desert tortoises in the southern part of the range of Gopherus morafkai are distinct, particularly where the tortoises occur in tropical thornscrub and tropical deciduous forest. Recent studies have shed light on the ecology, morphology and genetics of these southern ‘desert’ tortoises. All evidence warrants recognition of this clade as a distinctive taxon and herein we describe it as Gopherus evgoodei sp. n. The description of the new species significantly reduces and limits the distribution of Gopherus morafkai to desertscrub habitat only. By contrast, Gopherus evgoodei sp. n. occurs in thornscrub and tropical deciduous forests only and this leaves it with the smallest range of the three sister species. We present conservation implications for the newly described Gopherus evgoodei, which already faces impending threats. PMID:27006625
Full Text Available Green turtle (Cheloniamydas; Testudines is included in the group of polyandryanimals, which is single female mated with many male. DNA polymorphism method generally considered to have a high degree of accuracy as compared to other methods to elucidate polyandry phenomena on many animals. In this research, three microsatellite loci were used to identify the number and frequency of genotypes per locus, the number and frequency alleles per locus, and genotypes and number of alleles in the nest. The purpose of this research was to study the reproductive pattern of Cheloniamydas and compensation eggs of males from hatchling’s population in turtle conservation area of Pangumbahan Coastal Park, West Java. The result showed that from 10 nests, we could find 37 genotypes with 11 alleles for D108 locus, 21 genotypes with 9 alleles for B103 locus, and 27 genotypes with 9 alleles for C102 locus. The alleles number of each nest was more than 5 alleles for 5 nests, and more than 4 alleles for the remaining nests. Based on the probabilities of alleles contribution of each parent, the green turtle was polyandry animals.
Full Text Available New nephron formation (nephrogenesis ceases in mammals around birth and is completely absent in adults. In contrast, postembryonic nephrogenesis is well documented in the mesonephric kidneys of fishes and amphibians. The transient mesonephros in reptiles (including birds and mammals is replaced by the metanephros during embryogenesis. Thus, one may speculate that postembryonic nephrogenesis is restricted to the mesonephric kidney. Previous reports have suggested the metanephros of non-avian reptiles (hereafter reptiles may continually form nephrons throughout life. We investigated the presence of adult nephrogenesis in reptiles by examining adult kidneys from several species including Trachemys scripta, Chrysemys picta, Boa constrictor, Tupinambis tegu, Anolis carolinensis, and Alligator mississipiensis among others. We found that all major reptilian groups (Testudines, Crocodylia, and Squamates showed the presence of adult nephrogenesis. The total amount of nephrogenesis varied greatly between species with turtles displaying the highest density of nephrogenesis. In contrast, we were unable to detect adult nephrogenesis in monotremes, and in the iguanid A. carolinensis. Nephron progenitor cells express the transcription factor Six2, which in mammals, becomes downregulated as the progenitor cell population is exhausted and nephrogenesis ends. Using the alligator as a model, we were able to detect Six2-positive cap mesenchyme cells in the adult kidney, which spatially correlated with areas of nephrogenesis. These results suggest that the metanephric kidney of reptiles has maintained the ability to continually grow new nephrons during postembryonic life, a process lost early in mammalian evolution, likely due to the persistence of a Six2-expressing progenitor cell population.
Full Text Available Complete mitochondrial (mt genome sequences with duplicate control regions (CRs have been detected in various animal species. In Testudines, duplicate mtCRs have been reported in the mtDNA of the Asian big-headed turtle, Platysternon megacephalum, which has three living subspecies. However, the evolutionary pattern of these CRs remains unclear. In this study, we report the completed sequences of duplicate CRs from 20 individuals belonging to three subspecies of this turtle and discuss the micro-evolutionary analysis of the evolution of duplicate CRs. Genetic distances calculated with MEGA 4.1 using the complete duplicate CR sequences revealed that within turtle subspecies, genetic distances between orthologous copies from different individuals were 0.63% for CR1 and 1.2% for CR2app:addword:respectively, and the average distance between paralogous copies of CR1 and CR2 was 4.8%. Phylogenetic relationships were reconstructed from the CR sequences, excluding the variable number of tandem repeats (VNTRs at the 3' end using three methods: neighbor-joining, maximum likelihood algorithm, and Bayesian inference. These data show that any two CRs within individuals were more genetically distant from orthologous genes in different individuals within the same subspecies. This suggests independent evolution of the two mtCRs within each P. megacephalum subspecies. Reconstruction of separate phylogenetic trees using different CR components (TAS, CD, CSB, and VNTRs suggested the role of recombination in the evolution of duplicate CRs. Consequently, recombination events were detected using RDP software with break points at ≈290 bp and ≈1,080 bp. Based on these results, we hypothesize that duplicate CRs in P. megacephalum originated from heterological ancestral recombination of mtDNA. Subsequent recombination could have resulted in homogenization during independent evolutionary events, thus maintaining the functions of duplicate CRs in the mtDNA of P
Sparkman, Amanda M; Schwartz, Tonia S; Madden, Jill A; Boyken, Scott E; Ford, Neil B; Serb, Jeanne M; Bronikowski, Anne M
Insulin-like growth factor-1 (IGF-1) is a member of the vertebrate insulin/insulin-like growth factor/relaxin gene family necessary for growth, reproduction, and survival at both the cellular and organismal level. Its sequence, protein structure, and function have been characterized in mammals, birds, and fish; however, a notable gap in our current knowledge of the function of IGF-1 and its molecular evolution is information in ectothermic reptiles. To address this disparity, we sequenced the coding region of IGF-1 in 11 reptile species-one crocodilian, three turtles, three lizards, and four snakes. Complete sequencing of the full mRNA transcript of a snake revealed the Ea-isoform, the predominant isoform of IGF-1 also reported in other vertebrate groups. A gene tree of the IGF-1 protein-coding region that incorporated sequences from diverse vertebrate groups showed similarity to the species phylogeny, with the exception of the placement of Testudines as sister group to Aves, due to their high nucleotide sequence similarity. In contrast, long-branch lengths indicate more rapid divergence in IGF-1 among lizards and snakes. Additionally, lepidosaurs (i.e., lizards and snakes) had higher rates of non-synonymous:synonymous substitutions (dN/dS) relative to archosaurs (i.e., birds and crocodilians) and turtles. Tests for positive selection on specific codons within branches and evaluation of the changes in the amino acid properties, suggested positive selection in lepidosaurs on the C domain of IGF-1, which is involved in binding affinity to the IGF-1 receptor. Predicted structural changes suggest that major alterations in protein structure and function may have occurred in reptiles. These data propose new insights into the molecular co-evolution of IGF-1 and its receptors, and ultimately the evolution of IGF-1's role in regulating life-history traits across vertebrates.
S. F. Perry
Full Text Available Comparison of avian and crocodylian lung structure suggests a basic archosaurian structural type, consisting of four rows of chambers that radiate independently from an intrapulmonary bronchus. The later structure is cartilage-reinforced cranially. Caudally, the cartilage reinforcement diminishes and the radiating pattern of tubular chambers becomes irregular. The presence of extensive pneumatic spaces in the dorsal vertebrae combined with the extremely large size of Brachiosaurus suggests that the dorsal margin of the lungs was strongly attached to the body wall, thus restricting lung inflation in this region. By analogy with similarly structured testudine lungs, it is highly probable that the ventral part of the very large and cavernous lungs consisted of sac-like chambers, which acted as air reservoirs and also ventilated the gas exchange tissue, which was concentrated dorsally. Die Strukturen der Vogel- und der Krokodillunge deuten auf einen basalen Bauplan der Archosaurierlunge hin, der aus vier Reihen von Kammern besteht, die von einem intrapulmonalen Bronchus entspringen. Dieser ist cranial knorpelig verstärkt. Caudal vermindert sich die knorpelige Verstärkung und das Verteilungsmuster der Kammern wird ungleichmäßig. Die Anwesenheit von pneumatischen Räumen in den Wirbelkörpern und die extreme Größe von Brachiosaurus deuten daraufhin, daß der dorsale Bereich der Lunge mit der Körperwand verbunden war. Dies schränkte die Belüftung der Lunge in dieser Region ein. In Analogie zu den ähnlich strukturierten Schildkrötenlungen ist es höchst wahrscheinlich, daß der ventrale Teil der sehr großen Lungen aus sackartigen Kammern bestand, die als Luftreservoir dienten und das dorsal liegende Gasaustauschgewebe ventilierten. doi:10.1002/mmng.1999.4860020104
Full Text Available Se presenta una caracterización de la fauna de reptiles del valle seco del río Magdalena en el departamento del Huila, así como la comparación faunística entre unidades de cobertura vegetal y épocas climáticas. Se registraron 31 especies pertenecientes a 30 géneros, 17 familias y dos órdenes. En el orden Squamata, la familia más diversa fue Teiidae (lagartijas con tres especies y Colubridae (serpientes con nueve. Para el orden Testudines se registró una especie. Con base en curvas de acumulación de especies y los estimadores no paramétricos Jackknife 2 y Bootstrap, para las lagartijas se obtuvo una alta representatividad en el muestreo (83% y 92% respectivamente, mientras que para las serpientes fue menor (75% y 82% respectivamente. La estructura y composición de los ensambles de reptiles en cada unidad de cobertura vegetal no fue significativamente diferente, en general se caracterizaron por presentar pocas especies con muchos individuos y numerosas especies raras. El arbustal presentó la mayor riqueza de especies seguido del bosque de ribera y los cultivos de cacao. En general la abundancia de reptiles fue mayor en la época de lluvias que en la época seca, sin embargo la manera en que responden las especies a las épocas climáticas está dada por sus características ecológicas, fisiológicas y comportamentales.
Rabi, Márton; Zhou, Chang-Fu; Wings, Oliver; Ge, Sun; Joyce, Walter G
Most turtles from the Middle and Late Jurassic of Asia are referred to the newly defined clade Xinjiangchelyidae, a group of mostly shell-based, generalized, small to mid-sized aquatic froms that are widely considered to represent the stem lineage of Cryptodira. Xinjiangchelyids provide us with great insights into the plesiomorphic anatomy of crown-cryptodires, the most diverse group of living turtles, and they are particularly relevant for understanding the origin and early divergence of the primary clades of extant turtles. Exceptionally complete new xinjiangchelyid material from the ?Qigu Formation of the Turpan Basin (Xinjiang Autonomous Province, China) provides new insights into the anatomy of this group and is assigned to Xinjiangchelys wusu n. sp. A phylogenetic analysis places Xinjiangchelys wusu n. sp. in a monophyletic polytomy with other xinjiangchelyids, including Xinjiangchelys junggarensis, X. radiplicatoides, X. levensis and X. latiens. However, the analysis supports the unorthodox, though tentative placement of xinjiangchelyids and sinemydids outside of crown-group Testudines. A particularly interesting new observation is that the skull of this xinjiangchelyid retains such primitive features as a reduced interpterygoid vacuity and basipterygoid processes. The homology of basipterygoid processes is confidently demonstrated based on a comprehensive review of the basicranial anatomy of Mesozoic turtles and a new nomenclatural system is introduced for the carotid canal system of turtles. The loss of the basipterygoid process and the bony enclosure of the carotid circulation system occurred a number of times independently during turtle evolution suggesting that the reinforcement of the basicranial region was essential for developing a rigid skull, thus paralleling the evolution of other amniote groups with massive skulls.
Full Text Available Comparative genome analysis of non-avian reptiles and amphibians provides important clues about the process of genome evolution in tetrapods. However, there is still only limited information available on the genome structures of these organisms. Consequently, the protokaryotypes of amniotes and tetrapods and the evolutionary processes of microchromosomes in tetrapods remain poorly understood. We constructed chromosome maps of functional genes for the Chinese soft-shelled turtle (Pelodiscus sinensis, the Siamese crocodile (Crocodylus siamensis, and the Western clawed frog (Xenopus tropicalis and compared them with genome and/or chromosome maps of other tetrapod species (salamander, lizard, snake, chicken, and human. This is the first report on the protokaryotypes of amniotes and tetrapods and the evolutionary processes of microchromosomes inferred from comparative genomic analysis of vertebrates, which cover all major non-avian reptilian taxa (Squamata, Crocodilia, Testudines. The eight largest macrochromosomes of the turtle and chicken were equivalent, and 11 linkage groups had also remained intact in the crocodile. Linkage groups of the chicken macrochromosomes were also highly conserved in X. tropicalis, two squamates, and the salamander, but not in human. Chicken microchromosomal linkages were conserved in the squamates, which have fewer microchromosomes than chicken, and also in Xenopus and the salamander, which both lack microchromosomes; in the latter, the chicken microchromosomal segments have been integrated into macrochromosomes. Our present findings open up the possibility that the ancestral amniotes and tetrapods had at least 10 large genetic linkage groups and many microchromosomes, which corresponded to the chicken macro- and microchromosomes, respectively. The turtle and chicken might retain the microchromosomes of the amniote protokaryotype almost intact. The decrease in number and/or disappearance of microchromosomes by repeated
Farmer, C G
The factors that explain the diverse arrangement of the major arteries of tetrapods are not known. Here, I aim to illuminate some of the underpinnings of these patterns. I review the variation in the sauropsid left, right, and dorsal aortae regarding the origin of the gastrointestinal blood vessels and the relative diameters of left and right aortae where they join together to form the dorsal aorta. I focus on these features because the quality of blood that flows through these aortae can vary depending on the state of cardiac shunting and the size of the vessel can provide insight into the quantity of blood borne by the vessels. I then place the information in a phyletic, historical, and ecological context. The plesiomorphic pattern is for the gastrointestinal vessels to arise as segmental arteries from the dorsal aorta, which is formed from the confluence of left and right aortae with similar diameters. The pattern is well conserved with only two major variations. First, in several clades of reptiles (testudines, crocodilians, lizards of the genera Varanus and Hydrosaurus) a substantial portion of the gastrointestinal arteries arises from the left aorta, leaving the diameter of the left aorta smaller than the right at their confluence. I hypothesize that this vascular arrangement facilitates growth by allowing more alkaline blood to flow to the somatic (body wall) and appendicular circulations, which may promote bone deposition and inhibit resorption, whereas hypercapnic, acidic blood flows to the digestive viscera, which may provide CO(2) as a substrate for the synthesis of gastric acid, bicarbonate, fatty acids, glutamine, purine rings, as well as glucose from lactate. Second, in some snakes and lizards with snake-like body forms, such as Amphisbaenidae, the diameters of left and right aortae are asymmetrical at their confluence with the left aorta exceeding the right, but in members of the amphibian order Gymnophiona the right generally exceeds the left. This
蔡波; 王跃招; 陈跃英; 李家堂
本文对中国爬行纲动物的分类体系和物种进行了系统的评估,规范了中文学名,给出了《中国爬行纲校正名录》,结果表明:中国现存爬行纲动物3目30科132属462种,其中鳄形目(Crocodylia)1科1属1种,龟鳖目(Testudines)6科18属33种,有鳞目(Squamata)蜥蜴亚目(Lacertilia) 10科41属189种,有鳞目蛇亚目(Serpentes) 13科72属239种.与《中国动物志爬行纲第一卷(总论、龟鳖目、鳄形目)》、《中国动物志爬行纲第二卷(有鳞目:蜥蜴亚目)》和《中国蛇类》相比,目和亚目无变化;科级水平新增5科,变更2科;属级水平新增23属,合并15属,变更6属;种级水平新增81种,变动2种;未收录同物异名12种、杂交6种、中国无分布7种.形态和分子系统发育研究结果在爬行动物不同分类阶元均有一定差异,文章对这些争议进行了讨论,并对名录的选择做了说明.
Full Text Available Con la participación de nueve especialistas de diferentes instituciones de Argentina y transcurridos más de 10 años desde la primera Lista Roja de la herpetofauna de la República Argentina realizada por la Asociación Herpetológica Argentina en el año 2000, se re-categorizaron los taxones de Testudines presentes en el país incorporando nueva información taxonómica, biológica y ecológica y, aplicando correcciones metodológicas. Como resultado, de los 14 taxones de tortugas continentales y marinas, nueve quedaron incluidas en la presente Lista Roja bajo algún grado de amenaza, tres En Peligro, tres Amenazadas y tres Vulnerables, mientras que dos fueron categorizadas como No Amenazadas y tres son Insuficientemente Conocidas. En la evaluación de las tres especies de tortugas marinas, que frecuentan las costas del Mar Argentino, se homologaron las categorías con las de IUCN (2011. Respecto de la categorización anterior, se agregó la especie Phrynops geoffranus (Insuficientemente Conocida, se sinonimizaron dos especies, disminuyó el número de especies En Peligro de tres a dos y se incrementó el de Vulnerables de una a tres. Los pocos cambios resultantes de la presente categorización de las tortugas de Argentina respecto de la anterior podría reflejar la escasez de estudios realizados sobre este grupo en la última década y la necesidad de promoverlos.
Chojnowski, Jena L; Braun, Edward L
Vertebrate genomes are comprised of isochores that are relatively long (>100 kb) regions with a relatively homogenous (either GC-rich or AT-rich) base composition and with rather sharp boundaries with neighboring isochores. Mammals and living archosaurs (birds and crocodilians) have heterogeneous genomes that include very GC-rich isochores. In sharp contrast, the genomes of amphibians and fishes are more homogeneous and they have a lower overall GC content. Because DNA with higher GC content is more thermostable, the elevated GC content of mammalian and archosaurian DNA has been hypothesized to be an adaptation to higher body temperatures. This hypothesis can be tested by examining structure of isochores across the reptilian clade, which includes the archosaurs, testudines (turtles), and lepidosaurs (lizards and snakes), because reptiles exhibit diverse body sizes, metabolic rates, and patterns of thermoregulation. This study focuses on a comparative analysis of a new set of expressed genes of the red-eared slider turtle and orthologs of the turtle genes in mammalian (human, mouse, dog, and opossum), archosaurian (chicken and alligator), and amphibian (western clawed frog) genomes. EST (expressed sequence tag) data from a turtle cDNA library enriched for genes that have specialized functions (developmental genes) revealed using the GC content of the third-codon-position to examine isochore structure requires careful consideration of the types of genes examined. The more highly expressed genes (e.g., housekeeping genes) are more likely to be GC-rich than are genes with specialized functions. However, the set of highly expressed turtle genes demonstrated that the turtle genome has a GC content that is intermediate between the GC-poor amphibians and the GC-rich mammals and archosaurs. There was a strong correlation between the GC content of all turtle genes and the GC content of other vertebrate genes, with the slope of the line describing this relationship also
Sonia Eugenia Palacios-Sánchez
hurricane impact, the three species share the available food resources in different proportions (bivalves, gastropods, barnacles and decapods, according to different strategies that enabled them to coexist and reduce interspecific competition. After the impact, the abundance of available prey decreased and the interespecific competition for food increased, leading to S. testudines and S. nephelus change their trophic spectrum (xiphosurans, amphipods, isopods and detritus and displacing S. splengleri of the inlet. The distribution of food resources was conditioned by the abundance and diversity of prey, as well as the adaptive response of each species. Rev. Biol. Trop. 58 (4: 1223-1235. Epub 2010 December 01.
Leslie Anais Castillo Cutiva
Full Text Available Characterization of the genetic diversity of Rhinoclemmys nasuta population inhabits Isla Palma (Malaga Bay, Valle del Caucawas carried out using three microsatellite systems (Cm72, Cm58 and Cm3. In this locality, individuals of R. nasutaare widely distributed in the inland streams and creeks system. 100 to 200 ìL of peripheral blood was taken off from 10 turtles in five streams of theisland, preserving samples in in a 0.5 M EDTA. DNA was extracted using Salting-out and Chelex 100 solutiontechniques. PCR amplified products were visualized and measured in polyacrylamide gels (19:1 to 8 % silver nitrate. Successful amplification products were obtained for all systems analyzed, two of which (Cm72 and Cm3 were found to be monomorphic, while the system Cm58had a high PIC (0.698 allowing to estimate the genetic diversity of this population. The observed heterozygosity was low (Ho = 0.26 and inbreeding indices FIS and FIT were high (0.67857 and 0.67881 , indicating an excess of homozygotes in each of the rivers and for the all population. The molecular analysis of variance suggested that there is no difference in genetic structure of the population (FST= 0.00075,p= 0.95112 . Therefore, the results suggest that the genetic diversity of R. nasutapopulation in Isla Palma was low and exhibited a highly inbred index.DIVERSIDAD GENÉTICA EN UNA POBLACION DE Rhinoclemmys nasuta (TESTUDINES:GEOEMYDIDAE ASOCIADA A UN AMBIENTE INSULAR DEL CHOCÓ BIOGEOGRÁFICOSe realizó la caracterización de la diversidad genética de la población de Rhinoclemmys nasuta, que habita en la localidad insular de Isla Palma, Bahía Málaga (Valle del Cauca, Colombia, utilizando tres sistemas de microsatélites (Cm72, Cm58 y Cm3. En esta localidad, R. nasuta se encuentra ampliamente distribuida en los sistemas de riachuelos y quebradas presentes. Se tomaron entre 100 a 200 µL de sangre periférica de 10 tortugas en cinco riachuelos, preservando las muestras en una solución 0
Contribución al conocimiento sobre la Taxonomía, distribución geográfica y ecología de la Tortuga "Bache" (Chelydra Serpentina Acutirostris Contribución al conocimiento sobre la Taxonomía, distribución geográfica y ecología de la Tortuga "Bache" (Chelydra Serpentina Acutirostris
longitud y el ancho de las marginales es constante en rossignonii pero no en acutirostris; además, no es fácilmente discernible, sino hay que tomar las dimensiones correspondientes.19. se propone un cambio del texto de las claves de determinación taxonómica para acutirostris y rossignonii.20. Se llega a la conclusión que las cuatro (4 formas de Chelydra no representan subespecies bien definidas, sino más bien populaciones locales ("demes" .21. El habitat de acutirostris consiste en las aguas mansas; su nicho ecológico, sin embargo, no está estrechamente restringido. Vive tanto en las lagunas, pozos y cabeceras de los caños dentro del habitat denominado como "selva tropical pluvial", como en los inmensos esteros de la costa del Pacífico y en los lagos y remansos de los valles abiertos (los cuales, posibleme han sido cubiertos parcialmente por bosques en los tiempos pasados.22. No existe un dimorfismo sexual discernible, en contraste con la mayoría de las demás Testudines; sin embargo, los ♂♂ crecen más grandes que las ♀♀.23. La época de celo se efectúa en junio, julio y noviembre, según las observaciones hechas con ejemplares mantenidos en cautividad.24. El período de anidación se efectúa en febrero en el bajo Atrato (Chocó; faltan los datos correspondientes de las demás regiones.25. Se encontraron hasta 27 huevos de forma circular o ligeramente ovaloide; miden de 3.5 - 3.3 cm a 3.9 - 3.2 cm. No se tomó el peso.26. La distribución geográfica de Chelydra serpentina acutirostris abarca el área entre Costa Rica, Panamá, Colombia y el Ecuador. El limite aproximado hacia el sur es la hoya del río Guayas, que desemboca en la bahia de Guayaquil.27. En Colombia se encuentra en las siguientes areas:a La costa del Pacífico, desde el río Valle (Chocó hasta el río Mataje (Nariño, situado en la frontera entre Colombia y Ecuador.b El Valle del Cauca propiamente dicho.c La region de Armenia (Quindío. Su migración hacia la hoya del Magdalena est