WorldWideScience

Sample records for genome project progress

  1. RadGenomics project

    Energy Technology Data Exchange (ETDEWEB)

    Iwakawa, Mayumi; Imai, Takashi; Harada, Yoshinobu [National Inst. of Radiological Sciences, Chiba (Japan). Frontier Research Center] [and others

    2002-06-01

    Human health is determined by a complex interplay of factors, predominantly between genetic susceptibility, environmental conditions and aging. The ultimate aim of the RadGenomics (Radiation Genomics) project is to understand the implications of heterogeneity in responses to ionizing radiation arising from genetic variation between individuals in the human population. The rapid progression of the human genome sequencing and the recent development of new technologies in molecular genetics are providing us with new opportunities to understand the genetic basis of individual differences in susceptibility to natural and/or artificial environmental factors, including radiation exposure. The RadGenomics project will inevitably lead to improved protocols for personalized radiotherapy and reductions in the potential side effects of such treatment. The project will contribute to future research into the molecular mechanisms of radiation sensitivity in humans and will stimulate the development of new high-throughput technologies for a broader application of biological and medical sciences. The staff members are specialists in a variety of fields, including genome science, radiation biology, medical science, molecular biology, and informatics, and have joined the RadGenomics project from various universities, companies, and research institutes. The project started in April 2001. (author)

  2. RadGenomics project

    International Nuclear Information System (INIS)

    Iwakawa, Mayumi; Imai, Takashi; Harada, Yoshinobu

    2002-01-01

    Human health is determined by a complex interplay of factors, predominantly between genetic susceptibility, environmental conditions and aging. The ultimate aim of the RadGenomics (Radiation Genomics) project is to understand the implications of heterogeneity in responses to ionizing radiation arising from genetic variation between individuals in the human population. The rapid progression of the human genome sequencing and the recent development of new technologies in molecular genetics are providing us with new opportunities to understand the genetic basis of individual differences in susceptibility to natural and/or artificial environmental factors, including radiation exposure. The RadGenomics project will inevitably lead to improved protocols for personalized radiotherapy and reductions in the potential side effects of such treatment. The project will contribute to future research into the molecular mechanisms of radiation sensitivity in humans and will stimulate the development of new high-throughput technologies for a broader application of biological and medical sciences. The staff members are specialists in a variety of fields, including genome science, radiation biology, medical science, molecular biology, and informatics, and have joined the RadGenomics project from various universities, companies, and research institutes. The project started in April 2001. (author)

  3. The Human Genome Project and Mental Retardation: An Educational Program. Final Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Sharon

    1999-05-03

    The Arc, a national organization on mental retardation, conducted an educational program for members, many of whom have a family member with a genetic condition causing mental retardation. The project informed members about the Human Genome scientific efforts, conducted training regarding ethical, legal and social implications and involved members in issue discussions. Short reports and fact sheets on genetic and ELSI topics were disseminated to 2,200 of the Arc's leaders across the country and to other interested individuals. Materials produced by the project can e found on the Arc's web site, TheArc.org.

  4. Genome Writing: Current Progress and Related Applications

    Directory of Open Access Journals (Sweden)

    Yueqiang Wang

    2018-02-01

    Full Text Available The ultimate goal of synthetic biology is to build customized cells or organisms to meet specific industrial or medical needs. The most important part of the customized cell is a synthetic genome. Advanced genomic writing technologies are required to build such an artificial genome. Recently, the partially-completed synthetic yeast genome project represents a milestone in this field. In this mini review, we briefly introduce the techniques for de novo genome synthesis and genome editing. Furthermore, we summarize recent research progresses and highlight several applications in the synthetic genome field. Finally, we discuss current challenges and future prospects. Keywords: Synthetic biology, Genome writing, Genome editing, Bioethics, Biosafety

  5. The human genome project: Information management, access, and regulation. Technical progress report, 1 April--31 August 1993

    Energy Technology Data Exchange (ETDEWEB)

    McInerney, J.D.; Micikas, L.B.

    1993-09-10

    Efforts are described to prepare educational materials including computer based as well as conventional type teaching materials for training interested high school and elementary students in aspects of Human Genome Project.

  6. The human genome project

    International Nuclear Information System (INIS)

    Worton, R.

    1996-01-01

    The Human Genome Project is a massive international research project, costing 3 to 5 billion dollars and expected to take 15 years, which will identify the all the genes in the human genome - i.e. the complete sequence of bases in human DNA. The prize will be the ability to identify genes causing or predisposing to disease, and in some cases the development of gene therapy, but this new knowledge will raise important ethical issues

  7. Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Block, S. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Cornwall, J. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Dally, W. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Dyson, F. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Fortson, N. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Joyce, G. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Kimble, H. J. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Lewis, N. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Max, C. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Prince, T. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Schwitters, R. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Weinberger, P. [The MITRE Corporation, McLean, VA (US). JASON Program Office; Woodin, W. H. [The MITRE Corporation, McLean, VA (US). JASON Program Office

    1998-01-04

    The study reviews Department of Energy supported aspects of the United States Human Genome Project, the joint National Institutes of Health/Department of Energy program to characterize all human genetic material, to discover the set of human genes, and to render them accessible for further biological study. The study concentrates on issues of technology, quality assurance/control, and informatics relevant to current effort on the genome project and needs beyond it. Recommendations are presented on areas of the genome program that are of particular interest to and supported by the Department of Energy.

  8. Parasite Genome Projects and the Trypanosoma cruzi Genome Initiative

    Directory of Open Access Journals (Sweden)

    Wim Degrave

    1997-11-01

    Full Text Available Since the start of the human genome project, a great number of genome projects on other "model" organism have been initiated, some of them already completed. Several initiatives have also been started on parasite genomes, mainly through support from WHO/TDR, involving North-South and South-South collaborations, and great hopes are vested in that these initiatives will lead to new tools for disease control and prevention, as well as to the establishment of genomic research technology in developing countries. The Trypanosoma cruzi genome project, using the clone CL-Brener as starting point, has made considerable progress through the concerted action of more than 20 laboratories, most of them in the South. A brief overview of the current state of the project is given

  9. The Chlamydomonas genome project: a decade on

    Science.gov (United States)

    Blaby, Ian K.; Blaby-Haas, Crysten; Tourasse, Nicolas; Hom, Erik F. Y.; Lopez, David; Aksoy, Munevver; Grossman, Arthur; Umen, James; Dutcher, Susan; Porter, Mary; King, Stephen; Witman, George; Stanke, Mario; Harris, Elizabeth H.; Goodstein, David; Grimwood, Jane; Schmutz, Jeremy; Vallon, Olivier; Merchant, Sabeeha S.; Prochnik, Simon

    2014-01-01

    The green alga Chlamydomonas reinhardtii is a popular unicellular organism for studying photosynthesis, cilia biogenesis and micronutrient homeostasis. Ten years since its genome project was initiated, an iterative process of improvements to the genome and gene predictions has propelled this organism to the forefront of the “omics” era. Housed at Phytozome, the Joint Genome Institute’s (JGI) plant genomics portal, the most up-to-date genomic data include a genome arranged on chromosomes and high-quality gene models with alternative splice forms supported by an abundance of RNA-Seq data. Here, we present the past, present and future of Chlamydomonas genomics. Specifically, we detail progress on genome assembly and gene model refinement, discuss resources for gene annotations, functional predictions and locus ID mapping between versions and, importantly, outline a standardized framework for naming genes. PMID:24950814

  10. The Ensembl genome database project.

    Science.gov (United States)

    Hubbard, T; Barker, D; Birney, E; Cameron, G; Chen, Y; Clark, L; Cox, T; Cuff, J; Curwen, V; Down, T; Durbin, R; Eyras, E; Gilbert, J; Hammond, M; Huminiecki, L; Kasprzyk, A; Lehvaslaiho, H; Lijnzaad, P; Melsopp, C; Mongin, E; Pettett, R; Pocock, M; Potter, S; Rust, A; Schmidt, E; Searle, S; Slater, G; Smith, J; Spooner, W; Stabenau, A; Stalker, J; Stupka, E; Ureta-Vidal, A; Vastrik, I; Clamp, M

    2002-01-01

    The Ensembl (http://www.ensembl.org/) database project provides a bioinformatics framework to organise biology around the sequences of large genomes. It is a comprehensive source of stable automatic annotation of the human genome sequence, with confirmed gene predictions that have been integrated with external data sources, and is available as either an interactive web site or as flat files. It is also an open source software engineering project to develop a portable system able to handle very large genomes and associated requirements from sequence analysis to data storage and visualisation. The Ensembl site is one of the leading sources of human genome sequence annotation and provided much of the analysis for publication by the international human genome project of the draft genome. The Ensembl system is being installed around the world in both companies and academic sites on machines ranging from supercomputers to laptops.

  11. Shippingport: Overall project progress

    International Nuclear Information System (INIS)

    Crimi, F.P.

    1989-01-01

    The Shippingport atomic power station (SAPS) consisted of the nuclear steam supply system and associated radioactive waste processing systems, which were owned by the US Department of Energy (DOE), and the balance of plant, owned by the Duquesne Light Company. The station is located at Shippingport, Pennsylvania, on 7 acres of land leased by DOE from Duquesne Light Company. The Shippingport Station Decommissioning Project (SSDP) is being performed under contract to the DOE by the General Electric Company (GE) and its preselected subcontractor, MK-Ferguson Company, as the decommissioning operations contractor (DOC). This paper describes the decommissioning work that has been accomplished since July 1988, and the project's cost and schedule status. As the first decommissioning of a commercial, full-scale nuclear power plant, the SSDP is expected to set the standards for the demolition of future nuclear power plants

  12. Human genomics projects and precision medicine.

    Science.gov (United States)

    Carrasco-Ramiro, F; Peiró-Pastor, R; Aguado, B

    2017-09-01

    The completion of the Human Genome Project (HGP) in 2001 opened the floodgates to a deeper understanding of medicine. There are dozens of HGP-like projects which involve from a few tens to several million genomes currently in progress, which vary from having specialized goals or a more general approach. However, data generation, storage, management and analysis in public and private cloud computing platforms have raised concerns about privacy and security. The knowledge gained from further research has changed the field of genomics and is now slowly permeating into clinical medicine. The new precision (personalized) medicine, where genome sequencing and data analysis are essential components, allows tailored diagnosis and treatment according to the information from the patient's own genome and specific environmental factors. P4 (predictive, preventive, personalized and participatory) medicine is introducing new concepts, challenges and opportunities. This review summarizes current sequencing technologies, concentrates on ongoing human genomics projects, and provides some examples in which precision medicine has already demonstrated clinical impact in diagnosis and/or treatment.

  13. Progressive Finland sees progress with nuclear projects

    Energy Technology Data Exchange (ETDEWEB)

    Dalton, David [NucNet, Brussels (Belgium)

    2016-02-15

    The Finnish Hanhikivi-1 reactor project is firmly on track and a licence has been granted for construction of a final disposal facility for spent nuclear fuel - the first final repository in the world to enter the construction phase. Significant progress has been made with plans for Finland to build its sixth nuclear reactor unit at Hanhikivi. Fennovoima's licensing manager Janne Liuko said the company expects to receive the construction licence for the Generation III+ Hanhikivi-1 plant in late 2017. The application was submitted to the Finnish Ministry of Employment and the Economy in June 2015.

  14. Skyhook project: progress report

    International Nuclear Information System (INIS)

    Ainsworth, E.J.; Prioleau, J.C.; Mahlmann, L.J.

    1986-01-01

    The authors have initiated two types of animal experiments to complement and extend the existing data base comparing low- and high-LET radiations, and the studies utilize the Harderian gland model and range-finding or pilot studies on life-span shortening after exposure to heavy charged particles characterized by different LETs. Results from the animal studies have yielded new information concerning relationships between LET and carcinogenic effects and the animal studies are of themselves complementary. The Harderian gland model yields information on in-vivo transformation under conditions of tumor promotion that result from excess pituitary hormones, while the life-span studies, involving no extraneous promotion, indicate interactions between tumor induction and expression. Comparison of tumorigenic results on the Harderian gland and life shortening responses are expected to be highly informative. The purpose of the Skyhook Project was to test the hypothesis that LET was an adequate predictor for life shortening and that low-energy charged particles characterized by a LET approximately the same as that for fission neutrons would produce quantitatively similar life-shortening results after single or multifractionated doses. Another purpose was to evaluate life shortening, using comparatively high doses, over a LET range from less than 1 to approximately 200 keV/μm

  15. The Pediatric Cancer Genome Project

    Science.gov (United States)

    Downing, James R; Wilson, Richard K; Zhang, Jinghui; Mardis, Elaine R; Pui, Ching-Hon; Ding, Li; Ley, Timothy J; Evans, William E

    2013-01-01

    The St. Jude Children’s Research Hospital–Washington University Pediatric Cancer Genome Project (PCGP) is participating in the international effort to identify somatic mutations that drive cancer. These cancer genome sequencing efforts will not only yield an unparalleled view of the altered signaling pathways in cancer but should also identify new targets against which novel therapeutics can be developed. Although these projects are still deep in the phase of generating primary DNA sequence data, important results are emerging and valuable community resources are being generated that should catalyze future cancer research. We describe here the rationale for conducting the PCGP, present some of the early results of this project and discuss the major lessons learned and how these will affect the application of genomic sequencing in the clinic. PMID:22641210

  16. DOE Joint Genome Institute 2008 Progress Report

    International Nuclear Information System (INIS)

    Gilbert, David

    2009-01-01

    dominated how sequencing was done in the last decade is being replaced by a variety of new processes and sequencing instruments. The JGI, with an increasing number of next-generation sequencers, whose throughput is 100- to 1,000-fold greater than the Sanger capillary-based sequencers, is increasingly focused in new directions on projects of scale and complexity not previously attempted. These new directions for the JGI come, in part, from the 2008 National Research Council report on the goals of the National Plant Genome Initiative as well as the 2007 National Research Council report on the New Science of Metagenomics. Both reports outline a crucial need for systematic large-scale surveys of the plant and microbial components of the biosphere as well as an increasing need for large-scale analysis capabilities to meet the challenge of converting sequence data into knowledge. The JGI is extensively discussed in both reports as vital to progress in these fields of major national interest. JGI's future plan for plants and microbes includes a systematic approach for investigation of these organisms at a scale requiring the special capabilities of the JGI to generate, manage, and analyze the datasets. JGI will generate and provide not only community access to these plant and microbial datasets, but also the tools for analyzing them. These activities will produce essential knowledge that will be needed if we are to be able to respond to the world's energy and environmental challenges. As the JGI Plant and Microbial programs advance, the JGI as a user facility is also evolving. The Institute has been highly successful in bending its technical and analytical skills to help users solve large complex problems of major importance, and that effort will continue unabated. The JGI will increasingly move from a central focus on 'one-off' user projects coming from small user communities to much larger scale projects driven by systematic and problem-focused approaches to selection of

  17. DOE Joint Genome Institute 2008 Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Gilbert, David

    2009-03-12

    -based sequencing process that dominated how sequencing was done in the last decade is being replaced by a variety of new processes and sequencing instruments. The JGI, with an increasing number of next-generation sequencers, whose throughput is 100- to 1,000-fold greater than the Sanger capillary-based sequencers, is increasingly focused in new directions on projects of scale and complexity not previously attempted. These new directions for the JGI come, in part, from the 2008 National Research Council report on the goals of the National Plant Genome Initiative as well as the 2007 National Research Council report on the New Science of Metagenomics. Both reports outline a crucial need for systematic large-scale surveys of the plant and microbial components of the biosphere as well as an increasing need for large-scale analysis capabilities to meet the challenge of converting sequence data into knowledge. The JGI is extensively discussed in both reports as vital to progress in these fields of major national interest. JGI's future plan for plants and microbes includes a systematic approach for investigation of these organisms at a scale requiring the special capabilities of the JGI to generate, manage, and analyze the datasets. JGI will generate and provide not only community access to these plant and microbial datasets, but also the tools for analyzing them. These activities will produce essential knowledge that will be needed if we are to be able to respond to the world's energy and environmental challenges. As the JGI Plant and Microbial programs advance, the JGI as a user facility is also evolving. The Institute has been highly successful in bending its technical and analytical skills to help users solve large complex problems of major importance, and that effort will continue unabated. The JGI will increasingly move from a central focus on 'one-off' user projects coming from small user communities to much larger scale projects driven by systematic and problem

  18. Genome projects and the functional-genomic era.

    Science.gov (United States)

    Sauer, Sascha; Konthur, Zoltán; Lehrach, Hans

    2005-12-01

    The problems we face today in public health as a result of the -- fortunately -- increasing age of people and the requirements of developing countries create an urgent need for new and innovative approaches in medicine and in agronomics. Genomic and functional genomic approaches have a great potential to at least partially solve these problems in the future. Important progress has been made by procedures to decode genomic information of humans, but also of other key organisms. The basic comprehension of genomic information (and its transfer) should now give us the possibility to pursue the next important step in life science eventually leading to a basic understanding of biological information flow; the elucidation of the function of all genes and correlative products encoded in the genome, as well as the discovery of their interactions in a molecular context and the response to environmental factors. As a result of the sequencing projects, we are now able to ask important questions about sequence variation and can start to comprehensively study the function of expressed genes on different levels such as RNA, protein or the cell in a systematic context including underlying networks. In this article we review and comment on current trends in large-scale systematic biological research. A particular emphasis is put on technology developments that can provide means to accomplish the tasks of future lines of functional genomics.

  19. The 1000 bull genome project

    Science.gov (United States)

    To meet growing global demands for high value protein from milk and meat, rates of genetic gain in domestic cattle must be accelerated. At the same time, animal health and welfare must be considered. The 1000 bull genomes project supports these goals by providing annotated sequence variants and ge...

  20. The Human Genome Diversity Project

    Energy Technology Data Exchange (ETDEWEB)

    Cavalli-Sforza, L. [Stanford Univ., CA (United States)

    1994-12-31

    The Human Genome Diversity Project (HGD Project) is an international anthropology project that seeks to study the genetic richness of the entire human species. This kind of genetic information can add a unique thread to the tapestry knowledge of humanity. Culture, environment, history, and other factors are often more important, but humanity`s genetic heritage, when analyzed with recent technology, brings another type of evidence for understanding species` past and present. The Project will deepen the understanding of this genetic richness and show both humanity`s diversity and its deep and underlying unity. The HGD Project is still largely in its planning stages, seeking the best ways to reach its goals. The continuing discussions of the Project, throughout the world, should improve the plans for the Project and their implementation. The Project is as global as humanity itself; its implementation will require the kinds of partnerships among different nations and cultures that make the involvement of UNESCO and other international organizations particularly appropriate. The author will briefly discuss the Project`s history, describe the Project, set out the core principles of the Project, and demonstrate how the Project will help combat the scourge of racism.

  1. Progress of JPDR decommissioning project

    International Nuclear Information System (INIS)

    Kiyota, M.; Yanagihara, S.

    1995-01-01

    The Japan Power Demonstration Reactor (JPDR) decommissioning project is progressively achieving its final goal; the project will be finished by March 1996 to release the JPDR's site into unrestricted use in a green field condition. The new techniques which developed or improved in R and D, the first phase of this program, have been successfully applied to the actual dismantling activities. Some decommissioning wastes have been managed as the first case of onsite shallow land burial based on the new regulatory frame of radioactive waste management. The experiences and the data obtained from the JPDR dismantling activities are expected to contribute to future decommissioning of commercial nuclear power plants. (author)

  2. All about the Human Genome Project (HGP)

    Science.gov (United States)

    ... Care Genomic Medicine Working Group New Horizons and Research Patient Management Policy and Ethics Issues Quick Links for Patient Care Education All About the Human Genome Project Fact Sheets Genetic Education Resources for ...

  3. [The human variome project and its progress].

    Science.gov (United States)

    Gao, Shan; Zhang, Ning; Zhang, Lei; Duan, Guang-You; Zhang, Tao

    2010-11-01

    The main goal of post genomics is to explain how the genome, the map of which has been constructed in the Human Genome Project, affacts activities of life. This leads to generate multiple "omics": structural genomics, functional genomics, proteomics, metabonomics, et al. In Jun. 2006, Melbourne, Australia, Human Genome Variation Society (HGVS) initiated the Human Variome Project (HVP) to collect all the sequence variation and polymorphism data worldwidely. HVP is to search and determine those mutations related with human diseases by association study between genetype and phenotype on the scale of genome level and other methods. Those results will be translated into clinical application. Considering the potential effects of this project on human health, this paper introduced its origin and main content in detail and discussed its meaning and prospect.

  4. Social implications of the Human Genome Project: Policy roundtable series and journals. Final progress report, March 15, 2001 - March 15, 2002

    Energy Technology Data Exchange (ETDEWEB)

    Seiguer, Erica

    2002-12-30

    This report reflects the activities of the Harvard Health Caucus at Harvard Medical School that were supported, in part, by the Department of Energy. The following policy roundtables and panels were held: Spring 2001 Policy Roundtable Series: The social implications of the Human Genome Project; Spring 2002 Policy Roundtable Series: Managing globalization to improve health; 13 February 2002 Keynote Address: The globalization of health; 25 February 2002 Healthier or Wealthier: Which comes first in the new global era?; 28 February 2002 The crisis of neglected diseases: Creating R&D incentives for diseases of developing countries; 7 March 2002 Health care education in the developing world: Bridging global and local health care practices; 20 March 2002 Building a legal framework for global health: How can the US and UN work to reduce global disparities?; 25 April 2002 The role of mass media and tobacco control efforts. Caucus organizational information is also included.

  5. Genomics and the human genome project: implications for psychiatry

    OpenAIRE

    Kelsoe, J R

    2004-01-01

    In the past decade the Human Genome Project has made extraordinary strides in understanding of fundamental human genetics. The complete human genetic sequence has been determined, and the chromosomal location of almost all human genes identified. Presently, a large international consortium, the HapMap Project, is working to identify a large portion of genetic variation in different human populations and the structure and relationship of these variants to each other. The Human Genome Project h...

  6. The human Genome project and the future of oncology

    International Nuclear Information System (INIS)

    Collins, Francis S.

    1996-01-01

    The Human Genome Project is an ambitious 15-year effort to devise maps and sequence of the 3-billion base pair human genome, including all 100,000 genes. The project is running ahead of schedule and under budget. Already the effects on progress in disease gene discovery have been dramatic, especially for cancer. The most appropriate uses of susceptibility testing for breast, ovarian, and colon cancer are being investigated in research protocols, and the need to prevent genetic discrimination in employment and health insurance is becoming more urgent. In the longer term, these gene discoveries are likely to usher in a new era of therapeutic molecular medicine

  7. Helminth genome projects: all or nothing

    Czech Academy of Sciences Publication Activity Database

    Lukeš, Julius; Horák, Aleš; Scholz, Tomáš

    2005-01-01

    Roč. 21, č. 6 (2005), s. 265-266 ISSN 1471-4922 Institutional research plan: CEZ:AV0Z60220518 Keywords : genome project * helminth * Dracunculus Subject RIV: EG - Zoology Impact factor: 4.526, year: 2005

  8. Origins of the Human Genome Project.

    Science.gov (United States)

    Watson, J D; Cook-Deegan, R M

    1991-01-01

    The Human Genome Project has become a reality. Building on a debate that dates back to 1985, several genome projects are now in full stride around the world, and more are likely to form in the next several years. Italy began its genome program in 1987, and the United Kingdom and U.S.S.R. in 1988. The European communities mounted several genome projects on yeast, bacteria, Drosophila, and Arabidospis thaliana (a rapidly growing plant with a small genome) in 1988, and in 1990 commenced a new 2-year program on the human genome. In the United States, we have completed the first year of operation of the National Center for Human Genome Research at the National Institutes of Health (NIH), now the largest single funding source for genome research in the world. There have been dedicated budgets focused on genome-scale research at NIH, the U.S. Department of Energy, and the Howard Hughes Medical Institute for several years, and results are beginning to accumulate. There were three annual meetings on genome mapping and sequencing at Cold Spring Harbor, New York, in the spring of 1988, 1989, and 1990; the talks have shifted from a discussion about how to approach problems to presenting results from experiments already performed. We have finally begun to work rather than merely talk. The purpose of genome projects is to assemble data on the structure of DNA in human chromosomes and those of other organisms. A second goal is to develop new technologies to perform mapping and sequencing. There have been impressive technical advances in the past 5 years since the debate about the human genome project began. We are on the verge of beginning pilot projects to test several approaches to sequencing long stretches of DNA, using both automation and manual methods. Ordered sets of yeast artificial chromosome and cosmid clones have been assembled to span more than 2 million base pairs of several human chromosomes, and a region of 10 million base pairs has been assembled for

  9. The life cycle of a genome project: perspectives and guidelines inspired by insect genome projects.

    Science.gov (United States)

    Papanicolaou, Alexie

    2016-01-01

    Many research programs on non-model species biology have been empowered by genomics. In turn, genomics is underpinned by a reference sequence and ancillary information created by so-called "genome projects". The most reliable genome projects are the ones created as part of an active research program and designed to address specific questions but their life extends past publication. In this opinion paper I outline four key insights that have facilitated maintaining genomic communities: the key role of computational capability, the iterative process of building genomic resources, the value of community participation and the importance of manual curation. Taken together, these ideas can and do ensure the longevity of genome projects and the growing non-model species community can use them to focus a discussion with regards to its future genomic infrastructure.

  10. Convergence of advances in genomics, team science, and repositories as drivers of progress in psychiatric genomics.

    Science.gov (United States)

    Lehner, Thomas; Senthil, Geetha; Addington, Anjené M

    2015-01-01

    After many years of unfilled promise, psychiatric genetics has seen an unprecedented number of successes in recent years. We hypothesize that the field has reached an inflection point through a confluence of four key developments: advances in genomics; the orientation of the scientific community around large collaborative team science projects; the development of sample and data repositories; and a policy framework for sharing and accessing these resources. We discuss these domains and their effect on scientific progress and provide a perspective on why we think this is only the beginning of a new era in scientific discovery. Published by Elsevier Inc.

  11. Extreme project. Progress report 2006

    International Nuclear Information System (INIS)

    Eyrolle, F.; Masson, O.; Charmasson, S.

    2007-01-01

    The E.X.T.R.E.M.E. project introduced in 2005 to the S.E.S.U.R.E. / L.E.R.C.M. has for objectives to acquire data on the consequences of the extreme climatic meteorological episodes on the distribution of the artificial radioisotopes within the various compartments of the geosphere. This report presents the synthesis of the actions developed in 2006 in positioning and in co financing of the project by means of regional or national research programs (C.A.R.M.A., E.X.T.R.E.M.A., E.C.C.O.R.E.V.I.), of data acquisition, valuation and scientific collaboration. (N.C.)

  12. Decomposable Mandrel Project. Progress report

    International Nuclear Information System (INIS)

    Letts, S.A.; Fearon, E.; Allison, L.; Buckley, S.; Saculla, M.; Cook, R.

    1995-01-01

    We report on our progress in developing a new technology to produce both Nova and NIF scale capsules using a depolymerizable mandrel. In this technique we use poly(α-methylstyrene) (PAMS) beads or shells as mandrels which are overcoated with plasma polymer. The poly(α-methylstyrene) mandrel is then thermally depolymerized to gas phase monomer which diffuses away through the more thermally stable plasma polymer coating, leaving a hollow shell. Since our last report we have concentrated on characterization of the final shell. Starting with PAMS bead mandrels leads to distorted pyrolyzed shells because of thermally induced creep of the CH coating. We found that plasma polymer coatings on hollow shell mandrels shrink isotropically during pyrolysis and maintain sphericity. We are now concentrating our efforts on the use of microencapsulated shells to prepare targets with buried diagnostic layers or inner wall surface texture

  13. Attitudes towards the Human Genome Project.

    Science.gov (United States)

    Shahroudi, Julie; Shaw, Geraldine

    Attitudes concerning the Human Genome Project were reported by faculty (N=40) and students (N=66) from a liberal arts college. Positive attitudes toward the project involved privacy, insurance and health, economic purposes, reproductive purposes, genetic counseling, religion and overall opinions. Negative attitudes were expressed regarding…

  14. Learning by Doing: the Progressive Novella Project.

    Science.gov (United States)

    Conroy, Michael G.

    1996-01-01

    States that the Progressive Novella Project for high school students involves the collaborative writing of a 35-50 page novella. Explains that prior to the actual writing process, students are educated in the basic elements of fiction writing. Describes the division of labor into groups. Comments that the results of the project are invariably…

  15. Genome evolution during progression to breast cancer

    KAUST Repository

    Newburger, D. E.

    2013-04-08

    Cancer evolution involves cycles of genomic damage, epigenetic deregulation, and increased cellular proliferation that eventually culminate in the carcinoma phenotype. Early neoplasias, which are often found concurrently with carcinomas and are histologically distinguishable from normal breast tissue, are less advanced in phenotype than carcinomas and are thought to represent precursor stages. To elucidate their role in cancer evolution we performed comparative whole-genome sequencing of early neoplasias, matched normal tissue, and carcinomas from six patients, for a total of 31 samples. By using somatic mutations as lineage markers we built trees that relate the tissue samples within each patient. On the basis of these lineage trees we inferred the order, timing, and rates of genomic events. In four out of six cases, an early neoplasia and the carcinoma share a mutated common ancestor with recurring aneuploidies, and in all six cases evolution accelerated in the carcinoma lineage. Transition spectra of somatic mutations are stable and consistent across cases, suggesting that accumulation of somatic mutations is a result of increased ancestral cell division rather than specific mutational mechanisms. In contrast to highly advanced tumors that are the focus of much of the current cancer genome sequencing, neither the early neoplasia genomes nor the carcinomas are enriched with potentially functional somatic point mutations. Aneuploidies that occur in common ancestors of neoplastic and tumor cells are the earliest events that affect a large number of genes and may predispose breast tissue to eventual development of invasive carcinoma.

  16. Genome evolution during progression to breast cancer

    KAUST Repository

    Newburger, D. E.; Kashef-Haghighi, D.; Weng, Z.; Salari, R.; Sweeney, R. T.; Brunner, A. L.; Zhu, S. X.; Guo, X.; Varma, S.; Troxell, M. L.; West, R. B.; Batzoglou, S.; Sidow, A.

    2013-01-01

    Cancer evolution involves cycles of genomic damage, epigenetic deregulation, and increased cellular proliferation that eventually culminate in the carcinoma phenotype. Early neoplasias, which are often found concurrently with carcinomas and are histologically distinguishable from normal breast tissue, are less advanced in phenotype than carcinomas and are thought to represent precursor stages. To elucidate their role in cancer evolution we performed comparative whole-genome sequencing of early neoplasias, matched normal tissue, and carcinomas from six patients, for a total of 31 samples. By using somatic mutations as lineage markers we built trees that relate the tissue samples within each patient. On the basis of these lineage trees we inferred the order, timing, and rates of genomic events. In four out of six cases, an early neoplasia and the carcinoma share a mutated common ancestor with recurring aneuploidies, and in all six cases evolution accelerated in the carcinoma lineage. Transition spectra of somatic mutations are stable and consistent across cases, suggesting that accumulation of somatic mutations is a result of increased ancestral cell division rather than specific mutational mechanisms. In contrast to highly advanced tumors that are the focus of much of the current cancer genome sequencing, neither the early neoplasia genomes nor the carcinomas are enriched with potentially functional somatic point mutations. Aneuploidies that occur in common ancestors of neoplastic and tumor cells are the earliest events that affect a large number of genes and may predispose breast tissue to eventual development of invasive carcinoma.

  17. Tunneling progress on the Yucca Mountain Project

    International Nuclear Information System (INIS)

    Hansmire, W.H.; Munzer, R.J.

    1996-01-01

    The current status of tunneling progress on the Yucca Mountain Project (YMP) is presented in this paper. The Exploratory Studies Facility (ESF), a key part of the YMP, has been long in development and construction is ongoing. This is a progress report on the tunneling aspects of the ESF as of January 1, 1996. For purposes of discussion in this summary, the tunneling has progressed in four general phases. The paper describes: tunneling in jointed rock under low stress; tunneling through the Bow Ridge Fault and soft rock; tunneling through the Imbricate Fault Zone; and Tunneling into the candidate repository formation

  18. Justice and the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, T.F.; Lappe, M. (eds.)

    1992-01-01

    Most of the essays gathered in this volume were first presented at a conference, Justice and the Human Genome, in Chicago in early November, 1991. The goal of the, conference was to consider questions of justice as they are and will be raised by the Human Genome Project. To achieve its goal of identifying and elucidating the challenges of justice inherent in genomic research and its social applications the conference drew together in one forum members from academia, medicine, and industry with interests divergent as rate-setting for insurance, the care of newborns, and the history of ethics. The essays in this volume address a number of theoretical and practical concerns relative to the meaning of genomic research.

  19. Justice and the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, T.F.; Lappe, M. [eds.

    1992-12-31

    Most of the essays gathered in this volume were first presented at a conference, Justice and the Human Genome, in Chicago in early November, 1991. The goal of the, conference was to consider questions of justice as they are and will be raised by the Human Genome Project. To achieve its goal of identifying and elucidating the challenges of justice inherent in genomic research and its social applications the conference drew together in one forum members from academia, medicine, and industry with interests divergent as rate-setting for insurance, the care of newborns, and the history of ethics. The essays in this volume address a number of theoretical and practical concerns relative to the meaning of genomic research.

  20. Human genome program report. Part 1, overview and progress

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-11-01

    This report contains Part 1 of a two-part report to reflect research and progress in the U.S. Department of Energy Human Genome Program from 1994 through 1996, with specified updates made just before publication. Part 1 consists of the program overview and report on progress.

  1. Genomic Prediction from Whole Genome Sequence in Livestock: The 1000 Bull Genomes Project

    DEFF Research Database (Denmark)

    Hayes, Benjamin J; MacLeod, Iona M; Daetwyler, Hans D

    Advantages of using whole genome sequence data to predict genomic estimated breeding values (GEBV) include better persistence of accuracy of GEBV across generations and more accurate GEBV across breeds. The 1000 Bull Genomes Project provides a database of whole genome sequenced key ancestor bulls....... In a dairy data set, predictions using BayesRC and imputed sequence data from 1000 Bull Genomes were 2% more accurate than with 800k data. We could demonstrate the method identified causal mutations in some cases. Further improvements will come from more accurate imputation of sequence variant genotypes...

  2. The Human Genome Project and Biology Education.

    Science.gov (United States)

    McInerney, Joseph D.

    1996-01-01

    Highlights the importance of the Human Genome Project in educating the public about genetics. Discusses four challenges that science educators must address: teaching for conceptual understanding, the nature of science, the personal and social impact of science and technology, and the principles of technology. Contains 45 references. (JRH)

  3. Implications of the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Kitcher, P.

    1998-11-01

    The Human Genome Project (HGP), launched in 1991, aims to map and sequence the human genome by 2006. During the fifteen-year life of the project, it is projected that $3 billion in federal funds will be allocated to it. The ultimate aims of spending this money are to analyze the structure of human DNA, to identify all human genes, to recognize the functions of those genes, and to prepare for the biology and medicine of the twenty-first century. The following summary examines some of the implications of the program, concentrating on its scientific import and on the ethical and social problems that it raises. Its aim is to expose principles that might be used in applying the information which the HGP will generate. There is no attempt here to translate the principles into detailed proposals for legislation. Arguments and discussion can be found in the full report, but, like this summary, that report does not contain any legislative proposals.

  4. Origins of the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Cook-Deegan, Robert

    1993-07-01

    The human genome project was borne of technology, grew into a science bureaucracy in the US and throughout the world, and is now being transformed into a hybrid academic and commercial enterprise. The next phase of the project promises to veer more sharply toward commercial application, harnessing both the technical prowess of molecular biology and the rapidly growing body of knowledge about DNA structure to the pursuit of practical benefits. Faith that the systematic analysis of DNA structure will prove to be a powerful research tool underlies the rationale behind the genome project. The notion that most genetic information is embedded in the sequence of CNA base pairs comprising chromosomes is a central tenet. A rough analogy is to liken an organism's genetic code to computer code. The coal of the genome project, in this parlance, is to identify and catalog 75,000 or more files (genes) in the software that directs construction of a self-modifying and self-replicating system -- a living organism.

  5. Origins of the Human Genome Project

    Science.gov (United States)

    Cook-Deegan, Robert (Affiliation: Institute of Medicine, National Academy of Sciences)

    1993-07-01

    The human genome project was borne of technology, grew into a science bureaucracy in the United States and throughout the world, and is now being transformed into a hybrid academic and commercial enterprise. The next phase of the project promises to veer more sharply toward commercial application, harnessing both the technical prowess of molecular biology and the rapidly growing body of knowledge about DNA structure to the pursuit of practical benefits. Faith that the systematic analysis of DNA structure will prove to be a powerful research tool underlies the rationale behind the genome project. The notion that most genetic information is embedded in the sequence of CNA base pairs comprising chromosomes is a central tenet. A rough analogy is to liken an organism's genetic code to computer code. The coal of the genome project, in this parlance, is to identify and catalog 75,000 or more files (genes) in the software that directs construction of a self-modifying and self-replicating system -- a living organism.

  6. 2013 Progress Report -- DOE Joint Genome Institute

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-11-01

    In October 2012, we introduced a 10-Year Strategic Vision [http://bit.ly/JGI-Vision] for the Institute. A central focus of this Strategic Vision is to bridge the gap between sequenced genomes and an understanding of biological functions at the organism and ecosystem level. This involves the continued massive-scale generation of sequence data, complemented by orthogonal new capabilities to functionally annotate these large sequence data sets. Our Strategic Vision lays out a path to guide our decisions and ensure that the evolving set of experimental and computational capabilities available to DOE JGI users will continue to enable groundbreaking science.

  7. A comprehensive crop genome research project: the Superhybrid Rice Genome Project in China.

    Science.gov (United States)

    Yu, Jun; Wong, Gane Ka-Shu; Liu, Siqi; Wang, Jian; Yang, Huanming

    2007-06-29

    In May 2000, the Beijing Institute of Genomics formally announced the launch of a comprehensive crop genome research project on rice genomics, the Chinese Superhybrid Rice Genome Project. SRGP is not simply a sequencing project targeted to a single rice (Oryza sativa L.) genome, but a full-swing research effort with an ultimate goal of providing inclusive basic genomic information and molecular tools not only to understand biology of the rice, both as an important crop species and a model organism of cereals, but also to focus on a popular superhybrid rice landrace, LYP9. We have completed the first phase of SRGP and provide the rice research community with a finished genome sequence of an indica variety, 93-11 (the paternal cultivar of LYP9), together with ample data on subspecific (between subspecies) polymorphisms, transcriptomes and proteomes, useful for within-species comparative studies. In the second phase, we have acquired the genome sequence of the maternal cultivar, PA64S, together with the detailed catalogues of genes uniquely expressed in the parental cultivars and the hybrid as well as allele-specific markers that distinguish parental alleles. Although SRGP in China is not an open-ended research programme, it has been designed to pave a way for future plant genomics research and application, such as to interrogate fundamentals of plant biology, including genome duplication, polyploidy and hybrid vigour, as well as to provide genetic tools for crop breeding and to carry along a social burden-leading a fight against the world's hunger. It began with genomics, the newly developed and industry-scale research field, and from the world's most populous country. In this review, we summarize our scientific goals and noteworthy discoveries that exploit new territories of systematic investigations on basic and applied biology of rice and other major cereal crops.

  8. Project Progress Assessment Report (PPAR) 2015

    International Nuclear Information System (INIS)

    Sall, Baba

    2015-01-01

    This evaluation reports reviews basic information, output achievement, equipment and human resources, comment and recommendations. It highlights outputs Fully achieved, those which are partially achieved or in progress and also non achieved outputs. Regarding comments and lessons learned, counterpart stated that the overall timeline of the project is respected, even if activities are delayed to adapt to technical, financial and human resources constraints. The results obtained are in line with expectations thanks to a rigorously respected scientific approach. The Collegial Coordination of the project (DSV-LNERV-CIRAD) and the TO are in phase on the conduct of the Project the collection of baseline data is a crucial phase in the implementation of tsetse control programs. It makes it possible to implement a good strategy. The scientific and technical rigor and the good atmosphere within the Project team are also to be retained.

  9. Project Progress Assessment Report (PPAR) 2012

    International Nuclear Information System (INIS)

    Sall, Baba

    2012-01-01

    This evaluation reports reviews basic information, output achievement, equipment and human resources, comment and recommendations. It highlights outputs Fully achieved, those which are partially achieved or in progress and also non achieved outputs. Regarding comments and lessons learned, counterpart stated that the overall timeline of the project is respected, even if activities are delayed to adapt to technical, financial and human resources constraints. The results obtained are in line with expectations thanks to a rigorously respected scientific approach. The Collegial Coordination of the project (DSV-LNERV-CIRAD) and the TO are in phase on the conduct of the Project the collection of baseline data is a crucial phase in the implementation of tsetse control programs. It makes it possible to implement a good strategy. The scientific and technical rigor and the good atmosphere within the Project team are also to be retained.

  10. The ALPHA project: A progress report

    International Nuclear Information System (INIS)

    Yadigaroglu, G.

    1995-01-01

    A review of the ALPHA project is presented, including a summary of progress and current status. The project comprises the experimental and analytical investigation of the long-term decay heat removal phenomena from the containment of the next generation of passive Advanced Light Water Reactors. In its present phase, the project is directed to the investigation of the Simplified Boiling Water Reactor (SBWR) Passive Containment Cooling system. The project includes separate-effects tests of containment phenomena (the LINX program). The effects of aerosols that may result from hypothetical severe accidents are also considered (the AIDA program). The construction and commissioning of the major ALPHA experimental facilities, PANDA, LINX-2 and AIDA is complete and the first series of tests are underway. Scaling studies have guided their design. Several small-scale experiments and studies have already produced valuable results

  11. An overview of the human genome project

    Energy Technology Data Exchange (ETDEWEB)

    Batzer, M.A.

    1994-01-01

    The human genome project is one of the most ambitious scientific projects to date, with the ultimate goal being a nucleotide sequence for all four billion bases of human DNA. In the process of determining the nucleotide sequence for each base, the location, function, and regulatory regions from the estimated 100,000 human genes will be identified. The genome project itself relies upon maps of the human genetic code derived from several different levels of resolution. Genetic linkage analysis provides a low resolution genome map. The information for genetic linkage maps is derived from the analysis of chromosome specific markers such as Sequence Tagged Sites (STSs), Variable Number of Tandem Repeats (VNTRs) or other polymorphic (highly informative) loci in a number of different-families. Using this information the location of an unknown disease gene can be limited to a region comprised of one million base pairs of DNA or less. After this point, one must construct or have access to a physical map of the region of interest. Physical mapping involves the construction of an ordered overlapping (contiguous) set of recombinant DNA clones. These clones may be derived from a number of different vectors including cosmids, Bacterial Artificial Chromosomes (BACs), P1 derived Artificial Chromosomes (PACs), somatic cell hybrids, or Yeast Artificial Chromosomes (YACs). The ultimate goal for physical mapping is to establish a completely overlapping (contiguous) set of clones for the entire genome. After a gene or region of interest has been localized using physical mapping the nucleotide sequence is determined. The overlap between genetic mapping, physical mapping and DNA sequencing has proven to be a powerful tool for the isolation of disease genes through positional cloning.

  12. Progress of the EAST project in China

    International Nuclear Information System (INIS)

    Wan, Y.X.; Wu, S.T.; Weng, P.D.; Li, J.G.; Gao, D.M.

    2005-01-01

    The Experimental Advanced Superconducting Tokamak (EAST) project is one of the National Mega-Projects of Science Research of China, which was approved by Chinese government in 1998. EAST is a full superconducting tokamak with an elongated plasma cross-section. The mission of the project is to widely investigate both of the physics and the technologies of advanced tokamak operations, especially the mechanism of power and particle handling for steady-state operations. The basic requirements for the EAST tokamak are full superconducting coils, suitable inductive current system, continuous working non-inductive current driven and heating systems, flexible operation scenarios, flexible J(r) and P(r) control, reliable and fast plasma positioning and shaping control, changeable plasma facing components, advanced divertor and diagnostics. Significant progress of the EAST project has been achieved during last two years. The R and D programs, mainly focused on the superconducting magnets, have processed successfully. The prototypes of main parts have been fabricated and qualified. Most of the key parts of the machine have been delivered to the assembly site. The assembly of the device has begun. It is planned to obtain the first plasma in 2005. The detail information of the testing results of superconducting magnets will be given in this paper. The assembly plan and the experimental plan will be introduced, too. (author)

  13. The Human Genome Project (HGP): dividends and challenges: a ...

    African Journals Online (AJOL)

    The Human Genome Project (HGP): dividends and challenges: a review. ... Genomic studies have given profound insights into the genetic organization of ... with it will be an essential part of modern medicine and biology for years to come.

  14. Teleosts Genomics: Progress and Prospects in Disease Prevention and Control

    Directory of Open Access Journals (Sweden)

    Hetron Mweemba Munang’andu

    2018-04-01

    Full Text Available Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.

  15. Teleosts Genomics: Progress and Prospects in Disease Prevention and Control.

    Science.gov (United States)

    Munang'andu, Hetron Mweemba; Galindo-Villegas, Jorge; David, Lior

    2018-04-04

    Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs) as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL) mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.

  16. Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project

    OpenAIRE

    Sliva, Anna; Yang, Huanming; Boeke, Jef D.; Mathews, Debra J. H.

    2015-01-01

    First introduced in 2011, the Synthetic Yeast Genome (Sc2.0) Project is a large international synthetic genomics project that will culminate in the first eukaryotic cell (Saccharomyces cerevisiae) with a fully synthetic genome. With collaborators from across the globe and from a range of institutions spanning from do-it-yourself biology (DIYbio) to commercial enterprises, it is important that all scientists working on this project are cognizant of the ethical and policy issues associated with...

  17. The modest beginnings of one genome project.

    Science.gov (United States)

    Kaback, David B

    2013-06-01

    One of the top things on a geneticist's wish list has to be a set of mutants for every gene in their particular organism. Such a set was produced for the yeast, Saccharomyces cerevisiae near the end of the 20th century by a consortium of yeast geneticists. However, the functional genomic analysis of one chromosome, its smallest, had already begun more than 25 years earlier as a project that was designed to define most or all of that chromosome's essential genes by temperature-sensitive lethal mutations. When far fewer than expected genes were uncovered, the relatively new field of molecular cloning enabled us and indeed, the entire community of yeast researchers to approach this problem more definitively. These studies ultimately led to cloning, genomic sequencing, and the production and phenotypic analysis of the entire set of knockout mutations for this model organism as well as a better concept of what defines an essential function, a wish fulfilled that enables this model eukaryote to continue at the forefront of research in modern biology.

  18. The Astronomy Genealogy Project: A Progress Report

    Science.gov (United States)

    Tenn, Joseph S.

    2016-01-01

    Although it is not yet visible, much progress has been made on the Astronomy Genealogy Project (AstroGen) since it was accepted as a project of the Historical Astronomy Division (HAD) three years ago. AstroGen will list the world's astronomers with information about their highest degrees and advisors. (In academic genealogy, your thesis advisor is your parent.) A small group (the AstroGen Team) has compiled a database of approximately 12,000 individuals who have earned doctorates with theses (dissertations) on topics in astronomy, astrophysics, cosmology, or planetary science. These include nearly all those submitted in Australia, Canada, the Netherlands, and New Zealand, and most of those in the United States (all through 2014 for most universities and all through 1990 for all). We are compiling more information than is maintained by the Mathematics Genealogy Project (MGP). In addition to name, degree, university, year of degree, and thesis advisor(s), all provided by MGP as well, we are including years of birth and death when available, mentors in addition to advisors, and links to the thesis when it is online and to the person's web page or obituary, when we can find it. We are still struggling with some questions, such as the boundaries of inclusion and whether or not to include subfields of astronomy. We believe that AstroGen will be a valuable resource for historians of science as well as a source of entertainment for those who like to look up their academic family trees. A dedicated researcher following links from AstroGen will be able to learn quite a lot about the careers of astronomy graduates of a particular university, country, or era. We are still seeking volunteers to enter the graduates of one or more universities.

  19. A 1000 Arab genome project to study the Emirati population.

    Science.gov (United States)

    Al-Ali, Mariam; Osman, Wael; Tay, Guan K; AlSafar, Habiba S

    2018-04-01

    Discoveries from the human genome, HapMap, and 1000 genome projects have collectively contributed toward the creation of a catalog of human genetic variations that has improved our understanding of human diversity. Despite the collegial nature of many of these genome study consortiums, which has led to the cataloging of genetic variations of different ethnic groups from around the world, genome data on the Arab population remains overwhelmingly underrepresented. The National Arab Genome project in the United Arab Emirates (UAE) aims to address this deficiency by using Next Generation Sequencing (NGS) technology to provide data to improve our understanding of the Arab genome and catalog variants that are unique to the Arab population of the UAE. The project was conceived to shed light on the similarities and differences between the Arab genome and those of the other ethnic groups.

  20. Skate Genome Project: Cyber-Enabled Bioinformatics Collaboration

    Science.gov (United States)

    Vincent, J.

    2011-01-01

    The Skate Genome Project, a pilot project of the North East Cyber infrastructure Consortium, aims to produce a draft genome sequence of Leucoraja erinacea, the Little Skate. The pilot project was designed to also develop expertise in large scale collaborations across the NECC region. An overview of the bioinformatics and infrastructure challenges faced during the first year of the project will be presented. Results to date and lessons learned from the perspective of a bioinformatics core will be highlighted.

  1. The Human Genome Project: how do we protect Australians?

    Science.gov (United States)

    Stott Despoja, N

    It is the moon landing of the nineties: the ambitious Human Genome Project--identifying the up to 100,000 genes that make up human DNA and the sequences of the three billion base-pairs that comprise the human genome. However, unlike the moon landing, the effects of the genome project will have a fundamental impact on the way we see ourselves and each other.

  2. The PLX- α Project: Progress and Plans

    Science.gov (United States)

    Hsu, S.; Witherspoon, F. D.; Cassibry, J.; Gilmore, M.; Samulyak, R.; Stoltz, P.; PLX-α Team

    2016-10-01

    The Plasma Liner Experiment-ALPHA (PLX- α) project aims to demonstrate the viability of spherically imploding plasma liners as a standoff driver for plasma-jet-driven magneto-inertial fusion (PJMIF). In the past year, progress has been made in designing and testing new contoured-gap coaxial guns, 3D model development and simulations (via Eulerian and Lagrangian hydrocodes) of PLX- α-relevant plasma-liner formation/implosion via up to 60 plasma jets ( 100 kJ of liner kinetic energy), 1D semi-analytic and numerical modeling of reactor-scale PJMIF (10s of MJ of liner kinetic energy), and preparation/upgrade of the PLX facility/diagnostics. The design goal for the coaxial guns is to form plasma jets of up to initial n 2 ×1016 cm-3, mass 5 mg, Vjet 50 km/s, rjet = 4 cm, and length 10 cm. The modeling research is assessing ram-pressure amplification and Mach-number degradation during liner convergence, evolution of liner non-uniformity amplitude and mode number, and exploration of PJMIF configurations with promising 1D and 2D fusion gains. Conical multi-jet-merging and full-4 π experiments will commence in Fall, 2016 and late 2017, respectively. Supported by the ARPA-E ALPHA Program.

  3. Fluor Hanford Project Focused Progress at Hanford

    International Nuclear Information System (INIS)

    HANSON, R.D.

    2000-01-01

    Fluor Hanford is making significant progress in accelerating cleanup at the Hanford site. This progress consistently aligns with a new strategic vision established by the U.S. Department of Energy's Richland Operations Office (RL)

  4. The impact of the human genome project on risk assessment

    International Nuclear Information System (INIS)

    Katarzyna Doerffer; Paul Unrau.

    1996-01-01

    The radiation protection approach to risk assessment assumes that cancer induction following radiation exposure is purely random. Present risk assessment methods derive risk from cancer incidence frequencies in exposed populations and associate disease outcomes totally with the level of exposure to ionizing red aeon. Exposure defines a risk factor that affects the probability of the disease outcome. But cancer risk can be affected by other risk factors such as underlying genetic factors (predisposition) of the exposed organism. These genetic risk factors are now becoming available for incorporation into ionizing radiation risk assessment Progress in the Human Genome Project (HOP) will lead to direct assays to measure the effects of genetic risk determinants in disease outcomes. When all genetic risk determinants are known and incorporated into risk assessment it will be possible to reevaluate the role of ionizing radiation in the causation of cancer. (author)

  5. Peanut (Arachis hypogaea Expressed Sequence Tag Project: Progress and Application

    Directory of Open Access Journals (Sweden)

    Suping Feng

    2012-01-01

    Full Text Available Many plant ESTs have been sequenced as an alternative to whole genome sequences, including peanut because of the genome size and complexity. The US peanut research community had the historic 2004 Atlanta Genomics Workshop and named the EST project as a main priority. As of August 2011, the peanut research community had deposited 252,832 ESTs in the public NCBI EST database, and this resource has been providing the community valuable tools and core foundations for various genome-scale experiments before the whole genome sequencing project. These EST resources have been used for marker development, gene cloning, microarray gene expression and genetic map construction. Certainly, the peanut EST sequence resources have been shown to have a wide range of applications and accomplished its essential role at the time of need. Then the EST project contributes to the second historic event, the Peanut Genome Project 2010 Inaugural Meeting also held in Atlanta where it was decided to sequence the entire peanut genome. After the completion of peanut whole genome sequencing, ESTs or transcriptome will continue to play an important role to fill in knowledge gaps, to identify particular genes and to explore gene function.

  6. GI-POP: a combinational annotation and genomic island prediction pipeline for ongoing microbial genome projects.

    Science.gov (United States)

    Lee, Chi-Ching; Chen, Yi-Ping Phoebe; Yao, Tzu-Jung; Ma, Cheng-Yu; Lo, Wei-Cheng; Lyu, Ping-Chiang; Tang, Chuan Yi

    2013-04-10

    Sequencing of microbial genomes is important because of microbial-carrying antibiotic and pathogenetic activities. However, even with the help of new assembling software, finishing a whole genome is a time-consuming task. In most bacteria, pathogenetic or antibiotic genes are carried in genomic islands. Therefore, a quick genomic island (GI) prediction method is useful for ongoing sequencing genomes. In this work, we built a Web server called GI-POP (http://gipop.life.nthu.edu.tw) which integrates a sequence assembling tool, a functional annotation pipeline, and a high-performance GI predicting module, in a support vector machine (SVM)-based method called genomic island genomic profile scanning (GI-GPS). The draft genomes of the ongoing genome projects in contigs or scaffolds can be submitted to our Web server, and it provides the functional annotation and highly probable GI-predicting results. GI-POP is a comprehensive annotation Web server designed for ongoing genome project analysis. Researchers can perform annotation and obtain pre-analytic information include possible GIs, coding/non-coding sequences and functional analysis from their draft genomes. This pre-analytic system can provide useful information for finishing a genome sequencing project. Copyright © 2012 Elsevier B.V. All rights reserved.

  7. INTEGRATION OF PROGRESS STRATEGY IN PROJECT MANAGEMENT

    OpenAIRE

    Дмитрий Георгиевич БЕЗУГЛЫЙ

    2015-01-01

    The methods and tools of the developed project promotion, the planning system of promotion strategy are suggested; the main approaches used in the project promotion have been identified. The conclusions about the importance, necessity and role in the promotion of a holistic project management system have been made.

  8. Comparative genomic data of the Avian Phylogenomics Project.

    Science.gov (United States)

    Zhang, Guojie; Li, Bo; Li, Cai; Gilbert, M Thomas P; Jarvis, Erich D; Wang, Jun

    2014-01-01

    genomic data that has been generated and used in our Avian Phylogenomics Project. To the best of our knowledge, the Avian Phylogenomics Project is the biggest vertebrate comparative genomics project to date. The genomic data presented here is expected to accelerate further analyses in many fields, including phylogenetics, comparative genomics, evolution, neurobiology, development biology, and other related areas.

  9. A computational genomics pipeline for prokaryotic sequencing projects.

    Science.gov (United States)

    Kislyuk, Andrey O; Katz, Lee S; Agrawal, Sonia; Hagen, Matthew S; Conley, Andrew B; Jayaraman, Pushkala; Nelakuditi, Viswateja; Humphrey, Jay C; Sammons, Scott A; Govil, Dhwani; Mair, Raydel D; Tatti, Kathleen M; Tondella, Maria L; Harcourt, Brian H; Mayer, Leonard W; Jordan, I King

    2010-08-01

    New sequencing technologies have accelerated research on prokaryotic genomes and have made genome sequencing operations outside major genome sequencing centers routine. However, no off-the-shelf solution exists for the combined assembly, gene prediction, genome annotation and data presentation necessary to interpret sequencing data. The resulting requirement to invest significant resources into custom informatics support for genome sequencing projects remains a major impediment to the accessibility of high-throughput sequence data. We present a self-contained, automated high-throughput open source genome sequencing and computational genomics pipeline suitable for prokaryotic sequencing projects. The pipeline has been used at the Georgia Institute of Technology and the Centers for Disease Control and Prevention for the analysis of Neisseria meningitidis and Bordetella bronchiseptica genomes. The pipeline is capable of enhanced or manually assisted reference-based assembly using multiple assemblers and modes; gene predictor combining; and functional annotation of genes and gene products. Because every component of the pipeline is executed on a local machine with no need to access resources over the Internet, the pipeline is suitable for projects of a sensitive nature. Annotation of virulence-related features makes the pipeline particularly useful for projects working with pathogenic prokaryotes. The pipeline is licensed under the open-source GNU General Public License and available at the Georgia Tech Neisseria Base (http://nbase.biology.gatech.edu/). The pipeline is implemented with a combination of Perl, Bourne Shell and MySQL and is compatible with Linux and other Unix systems.

  10. The Human Genome Project: big science transforms biology and medicine.

    Science.gov (United States)

    Hood, Leroy; Rowen, Lee

    2013-01-01

    The Human Genome Project has transformed biology through its integrated big science approach to deciphering a reference human genome sequence along with the complete sequences of key model organisms. The project exemplifies the power, necessity and success of large, integrated, cross-disciplinary efforts - so-called 'big science' - directed towards complex major objectives. In this article, we discuss the ways in which this ambitious endeavor led to the development of novel technologies and analytical tools, and how it brought the expertise of engineers, computer scientists and mathematicians together with biologists. It established an open approach to data sharing and open-source software, thereby making the data resulting from the project accessible to all. The genome sequences of microbes, plants and animals have revolutionized many fields of science, including microbiology, virology, infectious disease and plant biology. Moreover, deeper knowledge of human sequence variation has begun to alter the practice of medicine. The Human Genome Project has inspired subsequent large-scale data acquisition initiatives such as the International HapMap Project, 1000 Genomes, and The Cancer Genome Atlas, as well as the recently announced Human Brain Project and the emerging Human Proteome Project.

  11. The Human Genome Project: An Imperative for International Collaboration.

    Science.gov (United States)

    Allende, J. E.

    1989-01-01

    Discussed is the Human Genome Project which aims to decipher the totality of the human genetic information. The historical background, the objectives, international cooperation, ethical discussion, and the role of UNESCO are included. (KR)

  12. Genome-wide association studies in asthma: progress and pitfalls

    Directory of Open Access Journals (Sweden)

    March ME

    2015-01-01

    Full Text Available Michael E March,1 Patrick MA Sleiman,1,2 Hakon Hakonarson1,2 1Center for Applied Genomics, Children's Hospital of Philadelphia Research Institute, 2Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA Abstract: Genetic studies of asthma have revealed that there is considerable heritability to the phenotype. An extensive history of candidate-gene studies has identified a long list of genes associated with immune function that are potentially involved in asthma pathogenesis. However, many of the results of candidate-gene studies have failed to be replicated, leaving in question the true impact of the implicated biological pathways on asthma. With the advent of genome-wide association studies, geneticists are able to examine the association of hundreds of thousands of genetic markers with a phenotype, allowing the hypothesis-free identification of variants associated with disease. Many such studies examining asthma or related phenotypes have been published, and several themes have begun to emerge regarding the biological pathways underpinning asthma. The results of many genome-wide association studies have currently not been replicated, and the large sample sizes required for this experimental strategy invoke difficulties with sample stratification and phenotypic heterogeneity. Recently, large collaborative groups of researchers have formed consortia focused on asthma, with the goals of sharing material and data and standardizing diagnosis and experimental methods. Additionally, research has begun to focus on genetic variants that affect the response to asthma medications and on the biology that generates the heterogeneity in the asthma phenotype. As this work progresses, it will move asthma patients closer to more specific, personalized medicine. Keywords: asthma, genetics, GWAS, pharmacogenetics, biomarkers

  13. USEPAM Project: The State of Progress

    DEFF Research Database (Denmark)

    Lund, Søren

    The paper represents the introductory speech at the 2nd regional workshop of the USEPAM project in Phnom Penh March 2005. It gives a review of the project's activities and achievements, and an introduction to the objectives, structure and programme of the workshop...

  14. The Human Genome Project: big science transforms biology and medicine

    OpenAIRE

    Hood, Leroy; Rowen, Lee

    2013-01-01

    The Human Genome Project has transformed biology through its integrated big science approach to deciphering a reference human genome sequence along with the complete sequences of key model organisms. The project exemplifies the power, necessity and success of large, integrated, cross-disciplinary efforts - so-called ‘big science’ - directed towards complex major objectives. In this article, we discuss the ways in which this ambitious endeavor led to the development of novel technologies and a...

  15. Progress of the HTR-10 project

    International Nuclear Information System (INIS)

    Zhong, D.; Xu, Y.

    1996-01-01

    This paper briefly introduces the main technical features and the design specifications of the HTR-10. Present status and main progress of the license applications, the design and manufacture of the main components and the engineering experiments as well as the construction of the HTR-10 are summarized. (author). 3 tabs

  16. National human genome projects: an update and an agenda.

    Science.gov (United States)

    An, Joon Yong

    2017-01-01

    Population genetic and human genetic studies are being accelerated with genome technology and data sharing. Accordingly, in the past 10 years, several countries have initiated genetic research using genome technology and identified the genetic architecture of the ethnic groups living in the corresponding country or suggested the genetic foundation of a social phenomenon. Genetic research has been conducted from epidemiological studies that previously described the health or disease conditions in defined population. This perspective summarizes national genome projects conducted in the past 10 years and introduces case studies to utilize genomic data in genetic research.

  17. EG-13GENOME-WIDE METHYLATION ANALYSIS IDENTIFIES GENOMIC DNA DEMETHYLATION DURING MALIGNANT PROGRESSION OF GLIOMAS

    Science.gov (United States)

    Saito, Kuniaki; Mukasa, Akitake; Nagae, Genta; Aihara, Koki; Otani, Ryohei; Takayanagi, Shunsaku; Omata, Mayu; Tanaka, Shota; Shibahara, Junji; Takahashi, Miwako; Momose, Toshimitsu; Shimamura, Teppei; Miyano, Satoru; Narita, Yoshitaka; Ueki, Keisuke; Nishikawa, Ryo; Nagane, Motoo; Aburatani, Hiroyuki; Saito, Nobuhito

    2014-01-01

    Low-grade gliomas often undergo malignant progression, and these transformations are a leading cause of death in patients with low-grade gliomas. However, the molecular mechanisms underlying malignant tumor progression are still not well understood. Recent evidence indicates that epigenetic deregulation is an important cause of gliomagenesis; therefore, we examined the impact of epigenetic changes during malignant progression of low-grade gliomas. Specifically, we used the Illumina Infinium Human Methylation 450K BeadChip to perform genome-wide DNA methylation analysis of 120 gliomas and four normal brains. This study sample included 25 matched-pairs of initial low-grade gliomas and recurrent tumors (temporal heterogeneity) and 20 of the 25 recurring tumors recurred as malignant progressions, and one matched-pair of newly emerging malignant lesions and pre-existing lesions (spatial heterogeneity). Analyses of methylation profiles demonstrated that most low-grade gliomas in our sample (43/51; 84%) had a CpG island methylator phenotype (G-CIMP). Remarkably, approximately 50% of secondary glioblastomas that had progressed from low-grade tumors with the G-CIMP status exhibited a characteristic partial demethylation of genomic DNA during malignant progression, but other recurrent gliomas showed no apparent change in DNA methylation pattern. Interestingly, we found that most loci that were demethylated during malignant progression were located outside of CpG islands. The information of histone modifications patterns in normal human astrocytes and embryonal stem cells also showed that the ratio of active marks at the site corresponding to DNA demethylated loci in G-CIMP-demethylated tumors was significantly lower; this finding indicated that most demethylated loci in G-CIMP-demethylated tumors were likely transcriptionally inactive. A small number of the genes that were upregulated and had demethylated CpG islands were associated with cell cycle-related pathway. In

  18. The Genome 10K Project: a way forward.

    Science.gov (United States)

    Koepfli, Klaus-Peter; Paten, Benedict; O'Brien, Stephen J

    2015-01-01

    The Genome 10K Project was established in 2009 by a consortium of biologists and genome scientists determined to facilitate the sequencing and analysis of the complete genomes of 10,000 vertebrate species. Since then the number of selected and initiated species has risen from ∼26 to 277 sequenced or ongoing with funding, an approximately tenfold increase in five years. Here we summarize the advances and commitments that have occurred by mid-2014 and outline the achievements and present challenges of reaching the 10,000-species goal. We summarize the status of known vertebrate genome projects, recommend standards for pronouncing a genome as sequenced or completed, and provide our present and future vision of the landscape of Genome 10K. The endeavor is ambitious, bold, expensive, and uncertain, but together the Genome 10K Consortium of Scientists and the worldwide genomics community are moving toward their goal of delivering to the coming generation the gift of genome empowerment for many vertebrate species.

  19. Geodynamics Project. US progress report, 1975

    Energy Technology Data Exchange (ETDEWEB)

    1976-01-01

    The recommendations of the US Geodynamics Committee relative to program activities are presented. US Program progress is reviewed in the following areas: fine structure of the crust and upper mantle; continuous seismic reflection profiling of the deep basement: Hardeman County, Texas; Mid-Atlantic Ridge - evolution of oceanic lithosphere; internal processes and properties; crystal growing; chemical differentiation of magmas; geodynamic modelling; magnetic problems; plate boundaries; plate interiors; geodynamic syntheses; and eustatic cycles of sea level changes. (MHR)

  20. Los Alamos Science: The Human Genome Project. Number 20, 1992

    Science.gov (United States)

    Cooper, N. G.; Shea, N. eds.

    1992-01-01

    This document provides a broad overview of the Human Genome Project, with particular emphasis on work being done at Los Alamos. It tries to emphasize the scientific aspects of the project, compared to the more speculative information presented in the popular press. There is a brief introduction to modern genetics, including a review of classic work. There is a broad overview of the Genome Project, describing what the project is, what are some of its major five-year goals, what are major technological challenges ahead of the project, and what can the field of biology, as well as society expect to see as benefits from this project. Specific results on the efforts directed at mapping chromosomes 16 and 5 are discussed. A brief introduction to DNA libraries is presented, bearing in mind that Los Alamos has housed such libraries for many years prior to the Genome Project. Information on efforts to do applied computational work related to the project are discussed, as well as experimental efforts to do rapid DNA sequencing by means of single-molecule detection using applied spectroscopic methods. The article introduces the Los Alamos staff which are working on the Genome Project, and concludes with brief discussions on ethical, legal, and social implications of this work; a brief glimpse of genetics as it may be practiced in the next century; and a glossary of relevant terms.

  1. Los Alamos Science: The Human Genome Project. Number 20, 1992

    Energy Technology Data Exchange (ETDEWEB)

    Cooper, N G; Shea, N [eds.

    1992-01-01

    This article provides a broad overview of the Human Genome Project, with particular emphasis on work being done at Los Alamos. It tries to emphasize the scientific aspects of the project, compared to the more speculative information presented in the popular press. There is a brief introduction to modern genetics, including a review of classic work. There is a broad overview of the Genome Project, describing what the project is, what are some of its major five-year goals, what are major technological challenges ahead of the project, and what can the field of biology, as well as society expect to see as benefits from this project. Specific results on the efforts directed at mapping chromosomes 16 and 5 are discussed. A brief introduction to DNA libraries is presented, bearing in mind that Los Alamos has housed such libraries for many years prior to the Genome Project. Information on efforts to do applied computational work related to the project are discussed, as well as experimental efforts to do rapid DNA sequencing by means of single-molecule detection using applied spectroscopic methods. The article introduces the Los Alamos staff which are working on the Genome Project, and concludes with brief discussions on ethical, legal, and social implications of this work; a brief glimpse of genetics as it may be practiced in the next century; and a glossary of relevant terms.

  2. Genomes to life project quarterly report June 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Heffelfinger, Grant S.

    2005-01-01

    This SAND report provides the technical progress through June 2004 of the Sandia-led project, ''Carbon Sequestration in Synechococcus Sp.: From Molecular Machines to Hierarchical Modeling'', funded by the DOE Office of Science Genomes to Life Program. Understanding, predicting, and perhaps manipulating carbon fixation in the oceans has long been a major focus of biological oceanography and has more recently been of interest to a broader audience of scientists and policy makers. It is clear that the oceanic sinks and sources of CO{sub 2} are important terms in the global environmental response to anthropogenic atmospheric inputs of CO{sub 2} and that oceanic microorganisms play a key role in this response. However, the relationship between this global phenomenon and the biochemical mechanisms of carbon fixation in these microorganisms is poorly understood. In this project, we will investigate the carbon sequestration behavior of Synechococcus Sp., an abundant marine cyanobacteria known to be important to environmental responses to carbon dioxide levels, through experimental and computational methods. This project is a combined experimental and computational effort with emphasis on developing and applying new computational tools and methods. Our experimental effort will provide the biology and data to drive the computational efforts and include significant investment in developing new experimental methods for uncovering protein partners, characterizing protein complexes, identifying new binding domains. We will also develop and apply new data measurement and statistical methods for analyzing microarray experiments. Computational tools will be essential to our efforts to discover and characterize the function of the molecular machines of Synechococcus. To this end, molecular simulation methods will be coupled with knowledge discovery from diverse biological data sets for high-throughput discovery and characterization of protein-protein complexes

  3. A decade of human genome project conclusion: Scientific diffusion about our genome knowledge.

    Science.gov (United States)

    Moraes, Fernanda; Góes, Andréa

    2016-05-06

    The Human Genome Project (HGP) was initiated in 1990 and completed in 2003. It aimed to sequence the whole human genome. Although it represented an advance in understanding the human genome and its complexity, many questions remained unanswered. Other projects were launched in order to unravel the mysteries of our genome, including the ENCyclopedia of DNA Elements (ENCODE). This review aims to analyze the evolution of scientific knowledge related to both the HGP and ENCODE projects. Data were retrieved from scientific articles published in 1990-2014, a period comprising the development and the 10 years following the HGP completion. The fact that only 20,000 genes are protein and RNA-coding is one of the most striking HGP results. A new concept about the organization of genome arose. The ENCODE project was initiated in 2003 and targeted to map the functional elements of the human genome. This project revealed that the human genome is pervasively transcribed. Therefore, it was determined that a large part of the non-protein coding regions are functional. Finally, a more sophisticated view of chromatin structure emerged. The mechanistic functioning of the genome has been redrafted, revealing a much more complex picture. Besides, a gene-centric conception of the organism has to be reviewed. A number of criticisms have emerged against the ENCODE project approaches, raising the question of whether non-conserved but biochemically active regions are truly functional. Thus, HGP and ENCODE projects accomplished a great map of the human genome, but the data generated still requires further in depth analysis. © 2016 by The International Union of Biochemistry and Molecular Biology, 44:215-223, 2016. © 2016 The International Union of Biochemistry and Molecular Biology.

  4. JET Project: progress in construction and management

    International Nuclear Information System (INIS)

    Bertolini, E.

    1978-01-01

    The JET Project of the European Community aims to construct and operate one of the largest Tokamak Devices foreseen at present in the field of Fusion Research. The aim of the experiment is to produce and study plasma conditions approaching those needed in a thermonuclear reactor and therefore will involve operation with deuterium--tritium gas

  5. Progress of JAERI neutron science project

    International Nuclear Information System (INIS)

    Oyama, Yukio

    1999-01-01

    Neutron Science Project was started at Japan Atomic Energy Research Institute since 1996 for promoting futuristic basic science and nuclear technology utilizing neutrons. For this purpose, research and developments of intense proton accelerator and spallation neutron target were initiated. The present paper describes the current status of such research and developments. (author)

  6. DOE Robotics Project. Summary of progress for 1991

    Energy Technology Data Exchange (ETDEWEB)

    1991-01-01

    This document provide the bimonthly progress reports on the Department of Energy (DOE) Robotics Project by the University of Michigan. Reports are provided for the time periods of December 90/January 91 through June 91/July 91. (FI)

  7. Human genome project: revolutionizing biology through leveraging technology

    Science.gov (United States)

    Dahl, Carol A.; Strausberg, Robert L.

    1996-04-01

    The Human Genome Project (HGP) is an international project to develop genetic, physical, and sequence-based maps of the human genome. Since the inception of the HGP it has been clear that substantially improved technology would be required to meet the scientific goals, particularly in order to acquire the complete sequence of the human genome, and that these technologies coupled with the information forthcoming from the project would have a dramatic effect on the way biomedical research is performed in the future. In this paper, we discuss the state-of-the-art for genomic DNA sequencing, technological challenges that remain, and the potential technological paths that could yield substantially improved genomic sequencing technology. The impact of the technology developed from the HGP is broad-reaching and a discussion of other research and medical applications that are leveraging HGP-derived DNA analysis technologies is included. The multidisciplinary approach to the development of new technologies that has been successful for the HGP provides a paradigm for facilitating new genomic approaches toward understanding the biological role of functional elements and systems within the cell, including those encoded within genomic DNA and their molecular products.

  8. The human genome project and the future of medical practice ...

    African Journals Online (AJOL)

    Contrary to the scepticism that characterised the planning stages of the human genome project, the technology and sequence data resulting from the project are set to revolutionise medical practice for good. The expected benefits include: enhanced discovery of disease genes, which will lead to improved knowledge on the ...

  9. Conjoint utility analysis of technical maturity and project progress of construction project

    Directory of Open Access Journals (Sweden)

    Ma Wei

    2016-01-01

    Full Text Available In this paper, taking construction project as the research object, the relationship between the project maturity index calculated by the construction project technical risks with different fine degree and the project progress index is studied, and the equilibrium relationship between the Party A’s utility curve and the Party B’s cost curve of using project maturity index and project progress index as the research variables is analyzed. The results show that, when the construction project technical risk division is more precise, the conjoint utility of the project's technical maturity index and the project progress is higher, and the project’s Party A and Party B two sides are closer to the optimal equilibrium. This shows that the construction project technical risk must be finely divided, and managed and controlled respectively, which will help to improve the conjoint utility of the project Party A and Party B two sides.

  10. The pig genome project has plenty to squeal about.

    Science.gov (United States)

    Fan, B; Gorbach, D M; Rothschild, M F

    2011-01-01

    Significant progress on pig genetics and genomics research has been witnessed in recent years due to the integration of advanced molecular biology techniques, bioinformatics and computational biology, and the collaborative efforts of researchers in the swine genomics community. Progress on expanding the linkage map has slowed down, but the efforts have created a higher-resolution physical map integrating the clone map and BAC end sequence. The number of QTL mapped is still growing and most of the updated QTL mapping results are available through PigQTLdb. Additionally, expression studies using high-throughput microarrays and other gene expression techniques have made significant advancements. The number of identified non-coding RNAs is rapidly increasing and their exact regulatory functions are being explored. A publishable draft (build 10) of the swine genome sequence was available for the pig genomics community by the end of December 2010. Build 9 of the porcine genome is currently available with Ensembl annotation; manual annotation is ongoing. These drafts provide useful tools for such endeavors as comparative genomics and SNP scans for fine QTL mapping. A recent community-wide effort to create a 60K porcine SNP chip has greatly facilitated whole-genome association analyses, haplotype block construction and linkage disequilibrium mapping, which can contribute to whole-genome selection. The future 'systems biology' that integrates and optimizes the information from all research levels can enhance the pig community's understanding of the full complexity of the porcine genome. These recent technological advances and where they may lead are reviewed. Copyright © 2011 S. Karger AG, Basel.

  11. Biorefinery Demonstration Project Final Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, David [University of Georgia Research Foundation, Inc., Athens, GA (United States)

    2015-10-20

    In this project we focused on various aspects of biorefinery technology development including algal-biorefinery technology, thermochemical conversion of biomass to bio-oils and biochar; we tested characteristics and applications of biochars and evaluated nutrient cycling with wastewater treatment by the coupling of algal culture systems and anaerobic digestion. Key results include a method for reducing water content of bio-oil through atomized alcohol addition. The effect included increasing the pH and reducing the viscosity and cloud point of the bio-oil. Low input biochar production systems were evaluated via literature reviews and direct experimental work. Additionally, emissions were evaluated and three biochar systems were compared via a life cycle analysis. Attached growth systems for both algal cultivation and algal harvesting were found to be superior to suspended growth cultures. Nutrient requirements for algal cultivation could be obtained by the recycling of anaerobic digester effluents, thus experimentally showing that these two systems could be directly coupled. Twenty-two journal articles and six intellectual property applications resulted from the cumulative work that this project contributed to programmatically.

  12. Genomes to Life Project Quartely Report October 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Heffelfinger, Grant S.; Martino, Anthony; Rintoul, Mark Daniel; Geist, Al; Gorin, Andrey; Xu, Ying; Palenik, Brian

    2005-02-01

    This SAND report provides the technical progress through October 2004 of the Sandia-led project, %22Carbon Sequestration in Synechococcus Sp.: From Molecular Machines to Hierarchical Modeling,%22 funded by the DOE Office of Science Genomes to Life Program. Understanding, predicting, and perhaps manipulating carbon fixation in the oceans has long been a major focus of biological oceanography and has more recently been of interest to a broader audience of scientists and policy makers. It is clear that the oceanic sinks and sources of CO2 are important terms in the global environmental response to anthropogenic atmospheric inputs of CO2 and that oceanic microorganisms play a key role in this response. However, the relationship between this global phenomenon and the biochemical mechanisms of carbon fixation in these microorganisms is poorly understood. In this project, we will investigate the carbon sequestration behavior of Synechococcus Sp., an abundant marine cyanobacteria known to be important to environmental responses to carbon dioxide levels, through experimental and computational methods. This project is a combined experimental and computational effort with emphasis on developing and applying new computational tools and methods. Our experimental effort will provide the biology and data to drive the computational efforts and include significant investment in developing new experimental methods for uncovering protein partners, characterizing protein complexes, identifying new binding domains. We will also develop and apply new data measurement and statistical methods for analyzing microarray experiments. Computational tools will be essential to our efforts to discover and characterize the function of the molecular machines of Synechococcus. To this end, molecular simulation methods will be coupled with knowledge discovery from diverse biological data sets for high-throughput discovery and characterization of protein-protein complexes. In addition, we will develop

  13. The research progress of genomic selection in livestock.

    Science.gov (United States)

    Li, Hong-wei; Wang, Rui-jun; Wang, Zhi-ying; Li, Xue-wu; Wang, Zhen-yu; Yanjun, Zhang; Rui, Su; Zhihong, Liu; Jinquan, Li

    2017-05-20

    With the development of gene chip and breeding technology, genomic selection in plants and animals has become research hotspots in recent years. Genomic selection has been extensively applied to all kinds of economic livestock, due to its high accuracy, short generation intervals and low breeding costs. In this review, we summarize genotyping technology and the methods for genomic breeding value estimation, the latter including the least square method, RR-BLUP, GBLUP, ssGBLUP, BayesA and BayesB. We also cover basic principles of genomic selection and compare their genetic marker ranges, genomic selection accuracy and operational speed. In addition, we list common indicators, methods and influencing factors that are related to genomic selection accuracy. Lastly, we discuss latest applications and the current problems of genomic selection at home and abroad. Importantly, we envision future status of genomic selection research, including multi-trait and multi-population genomic selection, as well as impact of whole genome sequencing and dominant effects on genomic selection. This review will provide some venues for other breeders to further understand genome selection.

  14. Imperial Valley Environmental Project: progress report

    Energy Technology Data Exchange (ETDEWEB)

    Phelps, P.L.; Anspaugh, L.R. (eds.)

    1977-10-19

    Progress is reported in six areas of research: air quality, water quality, ecosystem quality, subsidence and seismicity, socioeconomic effects, and integrated assessment. A major goal of the air quality element is to evaluate the rate of emission of H/sub 2/S, CO/sub 2/, H/sub 2/, N/sub 2/, CH/sub 4/, and C/sub 2/H/sub 6/ from the operation of the geothermal loop experimental facility at Niland. Concentrations of H/sub 2/S were found to vary between 1500 to 4900 ppM by volume at the Niland facility. To distinguish between geothermal fluids and other waters, extensive sampling networks were established. A major accomplishment was the installation of a high-resolution subsidence-detection network in the Salton Sea geothermal field area, centered on the test facility at Niland. A major effort went into establishing a background of data needed for subsequent impact assessments related to socioeconomic issues raised by geothermal developments. Underway are a set of geothermal energy scenarios that include power development schedules, technology characterizations, and considerations of power-plant-siting criteria. A Gaussian air-pollution model was modified for use in preliminary air-quality assessments. A crop-growth model was developed to evaluate impacts of gases released from geothermal operations on various agricultural crops. Work is also reported on the legal analysis of geothermal legislation and the legal aspects of water-supply utilization. Remote sensing was directed primarily at the Salton Sea, Heber, Brawley, and East Mesa KGRAs. However, large-format photography of the entire Salton Trough was completed. Thermal and multispectral imaging was done for several selected sites in the Salton Sea KGRA. (JGB)

  15. The OPTHER Project: Progress toward the THz Amplifier

    DEFF Research Database (Denmark)

    Paoloni, C; Brunetti, F; Di Carlo, A

    2011-01-01

    This paper describes the status of the OPTHER (OPtically driven TeraHertz AmplifiERs) project and progress toward the THz amplifier realization. This project represents a considerable advancement in the field of high frequency amplification. The design and realization of a THz amplifier within...... this project is a consolidation of efforts at the international level from the leading scientific and industrial European organizations working with vacuum electronics....

  16. US company's new nuclear project progresses

    International Nuclear Information System (INIS)

    Archie, Jeffrey B.

    2011-01-01

    Operated by South Carolina Electric and Gas Company in the southeastern United States, V.C. Summer Nuclear Station has maintained a reputation of excellence for more than 25 years. The key to its success: placing safety as its first priority, keeping a keen focus on operations, establishing a culture of continuous learning, and embracing collaboration with industry peers. This philosophy guides its new nuclear project, which SCE and G has undertaken to meet the need for baseload generation in its service area in a manner that is clean, safe, reliable, and efficient. In the process, SCE and G is generating jobs and boosting the economy. Site preparation has been under way since 2009. SCE and G anticipates receiving its combined construction and operating license from the Nuclear Regulatory Commission, the independent, federal agency that oversees commercial nuclear power plants in the U.S., in late 2011 or early 2012, which will allow major construction to commence. The 2 new reactors are scheduled to begin commercial operation in 2016 and 2019, respectively, placing V.C. Summer at the forefront of a U.S. nuclear renaissance and carrying its legacy of excellence into the future. (orig.)

  17. Harvard Personal Genome Project: lessons from participatory public research

    Science.gov (United States)

    2014-01-01

    Background Since its initiation in 2005, the Harvard Personal Genome Project has enrolled thousands of volunteers interested in publicly sharing their genome, health and trait data. Because these data are highly identifiable, we use an ‘open consent’ framework that purposefully excludes promises about privacy and requires participants to demonstrate comprehension prior to enrollment. Discussion Our model of non-anonymous, public genomes has led us to a highly participatory model of researcher-participant communication and interaction. The participants, who are highly committed volunteers, self-pursue and donate research-relevant datasets, and are actively engaged in conversations with both our staff and other Personal Genome Project participants. We have quantitatively assessed these communications and donations, and report our experiences with returning research-grade whole genome data to participants. We also observe some of the community growth and discussion that has occurred related to our project. Summary We find that public non-anonymous data is valuable and leads to a participatory research model, which we encourage others to consider. The implementation of this model is greatly facilitated by web-based tools and methods and participant education. Project results are long-term proactive participant involvement and the growth of a community that benefits both researchers and participants. PMID:24713084

  18. Harvard Personal Genome Project: lessons from participatory public research.

    Science.gov (United States)

    Ball, Madeleine P; Bobe, Jason R; Chou, Michael F; Clegg, Tom; Estep, Preston W; Lunshof, Jeantine E; Vandewege, Ward; Zaranek, Alexander; Church, George M

    2014-02-28

    Since its initiation in 2005, the Harvard Personal Genome Project has enrolled thousands of volunteers interested in publicly sharing their genome, health and trait data. Because these data are highly identifiable, we use an 'open consent' framework that purposefully excludes promises about privacy and requires participants to demonstrate comprehension prior to enrollment. Our model of non-anonymous, public genomes has led us to a highly participatory model of researcher-participant communication and interaction. The participants, who are highly committed volunteers, self-pursue and donate research-relevant datasets, and are actively engaged in conversations with both our staff and other Personal Genome Project participants. We have quantitatively assessed these communications and donations, and report our experiences with returning research-grade whole genome data to participants. We also observe some of the community growth and discussion that has occurred related to our project. We find that public non-anonymous data is valuable and leads to a participatory research model, which we encourage others to consider. The implementation of this model is greatly facilitated by web-based tools and methods and participant education. Project results are long-term proactive participant involvement and the growth of a community that benefits both researchers and participants.

  19. Sequencing of a new target genome: the Pediculus humanus humanus (Phthiraptera: Pediculidae) genome project.

    Science.gov (United States)

    Pittendrigh, B R; Clark, J M; Johnston, J S; Lee, S H; Romero-Severson, J; Dasch, G A

    2006-11-01

    The human body louse, Pediculus humanus humanus (L.), and the human head louse, Pediculus humanus capitis, belong to the hemimetabolous order Phthiraptera. The body louse is the primary vector that transmits the bacterial agents of louse-borne relapsing fever, trench fever, and epidemic typhus. The genomes of the bacterial causative agents of several of these aforementioned diseases have been sequenced. Thus, determining the body louse genome will enhance studies of host-vector-pathogen interactions. Although not important as a major disease vector, head lice are of major social concern. Resistance to traditional pesticides used to control head and body lice have developed. It is imperative that new molecular targets be discovered for the development of novel compounds to control these insects. No complete genome sequence exists for a hemimetabolous insect species primarily because hemimetabolous insects often have large (2000 Mb) to very large (up to 16,300 Mb) genomes. Fortuitously, we determined that the human body louse has one of the smallest genome sizes known in insects, suggesting it may be a suitable choice as a minimal hemimetabolous genome in which many genes have been eliminated during its adaptation to human parasitism. Because many louse species infest birds and mammals, the body louse genome-sequencing project will facilitate studies of their comparative genomics. A 6-8X coverage of the body louse genome, plus sequenced expressed sequence tags, should provide the entomological, evolutionary biology, medical, and public health communities with useful genetic information.

  20. Freedom and Responsibility in Synthetic Genomics: The Synthetic Yeast Project.

    Science.gov (United States)

    Sliva, Anna; Yang, Huanming; Boeke, Jef D; Mathews, Debra J H

    2015-08-01

    First introduced in 2011, the Synthetic Yeast Genome (Sc2.0) PROJECT is a large international synthetic genomics project that will culminate in the first eukaryotic cell (Saccharomyces cerevisiae) with a fully synthetic genome. With collaborators from across the globe and from a range of institutions spanning from do-it-yourself biology (DIYbio) to commercial enterprises, it is important that all scientists working on this project are cognizant of the ethical and policy issues associated with this field of research and operate under a common set of principles. In this commentary, we survey the current ethics and regulatory landscape of synthetic biology and present the Sc2.0 Statement of Ethics and Governance to which all members of the project adhere. This statement focuses on four aspects of the Sc2.0 PROJECT: societal benefit, intellectual property, safety, and self-governance. We propose that such project-level agreements are an important, valuable, and flexible model of self-regulation for similar global, large-scale synthetic biology projects in order to maximize the benefits and minimize potential harms. Copyright © 2015 by the Genetics Society of America.

  1. Progress of scientific researches and project of CSR in IMP

    International Nuclear Information System (INIS)

    Jin Genming

    2004-01-01

    The article reviews the recent progress of the scientific researches including synthesis of new nuclides, investigations of the isospin effects in heavy ion collisions, studies of the nuclear structure in high spin states and the applications of heavy ion beams to other scientific researches, such as biology and material science. It also gives a brief introduction of the development of the design and progress of the new project of heavy ion cooling storage ring (CSR) of Lanzhou. (author)

  2. Forecasting of Radiation Belts: Results From the PROGRESS Project.

    Science.gov (United States)

    Balikhin, M. A.; Arber, T. D.; Ganushkina, N. Y.; Walker, S. N.

    2017-12-01

    Forecasting of Radiation Belts: Results from the PROGRESS Project. The overall goal of the PROGRESS project, funded in frame of EU Horizon2020 programme, is to combine first principles based models with the systems science methodologies to achieve reliable forecasts of the geo-space particle radiation environment.The PROGRESS incorporates three themes : The propagation of the solar wind to L1, Forecast of geomagnetic indices, and forecast of fluxes of energetic electrons within the magnetosphere. One of the important aspects of the PROGRESS project is the development of statistical wave models for magnetospheric waves that affect the dynamics of energetic electrons such as lower band chorus, hiss and equatorial noise. The error reduction ratio (ERR) concept has been used to optimise the set of solar wind and geomagnetic parameters for organisation of statistical wave models for these emissions. The resulting sets of parameters and statistical wave models will be presented and discussed. However the ERR analysis also indicates that the combination of solar wind and geomagnetic parameters accounts for only part of the variance of the emissions under investigation (lower band chorus, hiss and equatorial noise). In addition, advances in the forecast of fluxes of energetic electrons, exploiting empirical models and the first principles IMPTAM model achieved by the PROGRESS project is presented.

  3. Genome editing: progress and challenges for medical applications

    Directory of Open Access Journals (Sweden)

    Dana Carroll

    2016-11-01

    Full Text Available Editorial summary The development of the CRISPR-Cas platform for genome editing has greatly simplified the process of making targeted genetic modifications. Applications of genome editing are expected to have a substantial impact on human therapies through the development of better animal models, new target discovery, and direct therapeutic intervention.

  4. SOCIAL PROGRESS AND ECONOMIC PROJECTS FOR DISADVANTAGED GROUPS IN ROMANIA

    Directory of Open Access Journals (Sweden)

    Aurelian Virgil BALUTA

    2016-12-01

    Full Text Available The paper aims to analyse the social progress, social politics, social law and successful projects applicable to the some disadvantaged groups: Roma people and woman. Inclusion of Roma people is analysed from economic point of view. The economic problems of equality by gender is presented in terms of labour rate and other relevant ratio. For Roma people the focus of analyse is also on labour involvement. The chapters of the communication are: introduction, literature review ( state of art in the field of social progress, theoretical background, tools for social progress in EU, economic inclusion of Roma population, economic equality by gender, conclusions.

  5. Reconsidering democracy. History of the Human Genome Project.

    NARCIS (Netherlands)

    Marli Huijer

    2003-01-01

    What options are open for people—citizens, politicians, and other nonscientists—to become actively involved in and anticipate new directions in the life sciences? In addressing this question, this article focuses on the start of the Human Genome Project (1985-1990). By contrasting various models of

  6. The Human Genome Project: Biology, Computers, and Privacy.

    Science.gov (United States)

    Cutter, Mary Ann G.; Drexler, Edward; Gottesman, Kay S.; Goulding, Philip G.; McCullough, Laurence B.; McInerney, Joseph D.; Micikas, Lynda B.; Mural, Richard J.; Murray, Jeffrey C.; Zola, John

    This module, for high school teachers, is the second of two modules about the Human Genome Project (HGP) produced by the Biological Sciences Curriculum Study (BSCS). The first section of this module provides background information for teachers about the structure and objectives of the HGP, aspects of the science and technology that underlie the…

  7. Reconsidering democracy - History of the human genome project

    NARCIS (Netherlands)

    Huijer, M

    What options are open for people-citizens, politicians, and other nonscientists-to become actively involved in and anticipate new directions in the life sciences? In addressing this question, this article focuses on the start of the Human Genome Project (1985-1990). By contrasting various models of

  8. Enhancing Biology Instruction with the Human Genome Project

    Science.gov (United States)

    Buxeda, Rosa J.; Moore-Russo, Deborah A.

    2003-01-01

    The Human Genome Project (HGP) is a recent scientific milestone that has received notable attention. This article shows how a biology course is using the HGP to enhance students' experiences by providing awareness of cutting edge research, with information on new emerging career options, and with opportunities to consider ethical questions raised…

  9. Comparing genetic variants detected in the 1000 genomes project ...

    Indian Academy of Sciences (India)

    Single-nucleotide polymorphisms (SNPs) determined based on SNP arrays from the international HapMap consortium (HapMap) and the genetic variants detected in the 1000 genomes project (1KGP) can serve as two references for genomewide association studies (GWAS). We conducted comparative analyses to provide ...

  10. Mapping our genes: The genome projects: How big, how fast

    Energy Technology Data Exchange (ETDEWEB)

    none,

    1988-04-01

    For the past 2 years, scientific and technical journals in biology and medicine have extensively covered a debate about whether and how to determine the function and order of human genes on human chromosomes and when to determine the sequence of molecular building blocks that comprise DNA in those chromosomes. In 1987, these issues rose to become part of the public agenda. The debate involves science, technology, and politics. Congress is responsible for /open quotes/writing the rules/close quotes/ of what various federal agencies do and for funding their work. This report surveys the points made so far in the debate, focusing on those that most directly influence the policy options facing the US Congress. Congressional interest focused on how to assess the rationales for conducting human genome projects, how to fund human genome projects (at what level and through which mechanisms), how to coordinate the scientific and technical programs of the several federal agencies and private interests already supporting various genome projects, and how to strike a balance regarding the impact of genome projects on international scientific cooperation and international economic competition in biotechnology. OTA prepared this report with the assistance of several hundred experts throughout the world. 342 refs., 26 figs., 11 tabs.

  11. Mapping Our Genes: The Genome Projects: How Big, How Fast

    Science.gov (United States)

    1988-04-01

    For the past 2 years, scientific and technical journals in biology and medicine have extensively covered a debate about whether and how to determine the function and order of human genes on human chromosomes and when to determine the sequence of molecular building blocks that comprise DNA in those chromosomes. In 1987, these issues rose to become part of the public agenda. The debate involves science, technology, and politics. Congress is responsible for ?writing the rules? of what various federal agencies do and for funding their work. This report surveys the points made so far in the debate, focusing on those that most directly influence the policy options facing the US Congress. Congressional interest focused on how to assess the rationales for conducting human genome projects, how to fund human genome projects (at what level and through which mechanisms), how to coordinate the scientific and technical programs of the several federal agencies and private interests already supporting various genome projects, and how to strike a balance regarding the impact of genome projects on international scientific cooperation and international economic competition in biotechnology. The Office of Technology Assessment (OTA) prepared this report with the assistance of several hundred experts throughout the world.

  12. The Cordoba and Wolsung projects: a progress report

    International Nuclear Information System (INIS)

    Brooks, G.L.

    1977-06-01

    The Cordoba and Wolsung projects mark the entry into the international sales arena of the standardized Canadian 600 MWe CANDU-PHW reactor design. The Cordoba station experienced a setback in the early stages when severe inflation in Argentina led to a renegotiation of the contract. However, following this, good progress has been made and the current forecast completion date in 1980 is expected to be achieved. The Wolsung project experienced difficulties early in the project due to site conditions, so that site work commenced some 9-10 months later than originally planned. These difficulties had predictable effects upon the progress of site-related engineering and it is expected that the project completion date will be somewhat delayed

  13. Coordinated international action to accelerate genome-to-phenome with FAANG, the Functional Annotation of Animal Genomes project : open letter

    NARCIS (Netherlands)

    Archibald, A.L.; Bottema, C.D.; Brauning, R.; Burgess, S.C.; Burt, D.W.; Casas, E.; Cheng, H.H.; Clarke, L.; Couldrey, C.; Dalrymple, B.P.; Elsik, C.G.; Foissac, S.; Giuffra, E.; Groenen, M.A.M.; Hayes, B.J.; Huang, L.S.; Khatib, H.; Kijas, J.W.; Kim, H.; Lunney, J.K.; McCarthy, F.M.; McEwan, J.; Moore, S.; Nanduri, B.; Notredame, C.; Palti, Y.; Plastow, G.S.; Reecy, J.M.; Rohrer, G.; Sarropoulou, E.; Schmidt, C.J.; Silverstein, J.; Tellam, R.L.; Tixier-Boichard, M.; Tosser-klopp, G.; Tuggle, C.K.; Vilkki, J.; White, S.N.; Zhao, S.; Zhou, H.

    2015-01-01

    We describe the organization of a nascent international effort, the Functional Annotation of Animal Genomes (FAANG) project, whose aim is to produce comprehensive maps of functional elements in the genomes of domesticated animal species.

  14. Integrated genomics of ovarian xenograft tumor progression and chemotherapy response

    International Nuclear Information System (INIS)

    Stuckey, Ashley; Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H; Hillenmeyer, Sara; Kim, Kyu K; Ritz, Anna; Singh, Rakesh K; Raphael, Benjamin J; Brard, Laurent

    2011-01-01

    Ovarian cancer is the most deadly gynecological cancer with a very poor prognosis. Xenograft mouse models have proven to be one very useful tool in testing candidate therapeutic agents and gene function in vivo. In this study we identify genes and gene networks important for the efficacy of a pre-clinical anti-tumor therapeutic, MT19c. In order to understand how ovarian xenograft tumors may be growing and responding to anti-tumor therapeutics, we used genome-wide mRNA expression and DNA copy number measurements to identify key genes and pathways that may be critical for SKOV-3 xenograft tumor progression. We compared SKOV-3 xenografts treated with the ergocalciferol derived, MT19c, to untreated tumors collected at multiple time points. Cell viability assays were used to test the function of the PPARγ agonist, Rosiglitazone, on SKOV-3 cell growth. These data indicate that a number of known survival and growth pathways including Notch signaling and general apoptosis factors are differentially expressed in treated vs. untreated xenografts. As tumors grow, cell cycle and DNA replication genes show increased expression, consistent with faster growth. The steroid nuclear receptor, PPARγ, was significantly up-regulated in MT19c treated xenografts. Surprisingly, stimulation of PPARγ with Rosiglitazone reduced the efficacy of MT19c and cisplatin suggesting that PPARγ is regulating a survival pathway in SKOV-3 cells. To identify which genes may be important for tumor growth and treatment response, we observed that MT19c down-regulates some high copy number genes and stimulates expression of some low copy number genes suggesting that these genes are particularly important for SKOV-3 xenograft growth and survival. We have characterized the time dependent responses of ovarian xenograft tumors to the vitamin D analog, MT19c. Our results suggest that PPARγ promotes survival for some ovarian tumor cells. We propose that a combination of regulated expression and copy number

  15. The African Genome Variation Project shapes medical genetics in Africa.

    Science.gov (United States)

    Gurdasani, Deepti; Carstensen, Tommy; Tekola-Ayele, Fasil; Pagani, Luca; Tachmazidou, Ioanna; Hatzikotoulas, Konstantinos; Karthikeyan, Savita; Iles, Louise; Pollard, Martin O; Choudhury, Ananyo; Ritchie, Graham R S; Xue, Yali; Asimit, Jennifer; Nsubuga, Rebecca N; Young, Elizabeth H; Pomilla, Cristina; Kivinen, Katja; Rockett, Kirk; Kamali, Anatoli; Doumatey, Ayo P; Asiki, Gershim; Seeley, Janet; Sisay-Joof, Fatoumatta; Jallow, Muminatou; Tollman, Stephen; Mekonnen, Ephrem; Ekong, Rosemary; Oljira, Tamiru; Bradman, Neil; Bojang, Kalifa; Ramsay, Michele; Adeyemo, Adebowale; Bekele, Endashaw; Motala, Ayesha; Norris, Shane A; Pirie, Fraser; Kaleebu, Pontiano; Kwiatkowski, Dominic; Tyler-Smith, Chris; Rotimi, Charles; Zeggini, Eleftheria; Sandhu, Manjinder S

    2015-01-15

    Given the importance of Africa to studies of human origins and disease susceptibility, detailed characterization of African genetic diversity is needed. The African Genome Variation Project provides a resource with which to design, implement and interpret genomic studies in sub-Saharan Africa and worldwide. The African Genome Variation Project represents dense genotypes from 1,481 individuals and whole-genome sequences from 320 individuals across sub-Saharan Africa. Using this resource, we find novel evidence of complex, regionally distinct hunter-gatherer and Eurasian admixture across sub-Saharan Africa. We identify new loci under selection, including loci related to malaria susceptibility and hypertension. We show that modern imputation panels (sets of reference genotypes from which unobserved or missing genotypes in study sets can be inferred) can identify association signals at highly differentiated loci across populations in sub-Saharan Africa. Using whole-genome sequencing, we demonstrate further improvements in imputation accuracy, strengthening the case for large-scale sequencing efforts of diverse African haplotypes. Finally, we present an efficient genotype array design capturing common genetic variation in Africa.

  16. The African Genome Variation Project shapes medical genetics in Africa

    Science.gov (United States)

    Gurdasani, Deepti; Carstensen, Tommy; Tekola-Ayele, Fasil; Pagani, Luca; Tachmazidou, Ioanna; Hatzikotoulas, Konstantinos; Karthikeyan, Savita; Iles, Louise; Pollard, Martin O.; Choudhury, Ananyo; Ritchie, Graham R. S.; Xue, Yali; Asimit, Jennifer; Nsubuga, Rebecca N.; Young, Elizabeth H.; Pomilla, Cristina; Kivinen, Katja; Rockett, Kirk; Kamali, Anatoli; Doumatey, Ayo P.; Asiki, Gershim; Seeley, Janet; Sisay-Joof, Fatoumatta; Jallow, Muminatou; Tollman, Stephen; Mekonnen, Ephrem; Ekong, Rosemary; Oljira, Tamiru; Bradman, Neil; Bojang, Kalifa; Ramsay, Michele; Adeyemo, Adebowale; Bekele, Endashaw; Motala, Ayesha; Norris, Shane A.; Pirie, Fraser; Kaleebu, Pontiano; Kwiatkowski, Dominic; Tyler-Smith, Chris; Rotimi, Charles; Zeggini, Eleftheria; Sandhu, Manjinder S.

    2015-01-01

    Given the importance of Africa to studies of human origins and disease susceptibility, detailed characterization of African genetic diversity is needed. The African Genome Variation Project provides a resource with which to design, implement and interpret genomic studies in sub-Saharan Africa and worldwide. The African Genome Variation Project represents dense genotypes from 1,481 individuals and whole-genome sequences from 320 individuals across sub-Saharan Africa. Using this resource, we find novel evidence of complex, regionally distinct hunter-gatherer and Eurasian admixture across sub-Saharan Africa. We identify new loci under selection, including loci related to malaria susceptibility and hypertension. We show that modern imputation panels (sets of reference genotypes from which unobserved or missing genotypes in study sets can be inferred) can identify association signals at highly differentiated loci across populations in sub-Saharan Africa. Using whole-genome sequencing, we demonstrate further improvements in imputation accuracy, strengthening the case for large-scale sequencing efforts of diverse African haplotypes. Finally, we present an efficient genotype array design capturing common genetic variation in Africa.

  17. The international arctic seas assessment project: Progress report

    International Nuclear Information System (INIS)

    Sjoeblom, K.L.; Linsley, G.S.

    1995-01-01

    The article provides some background information on wastes dumped into the Arctic Seas and describes the progress made within the framework of International Arctic Seas Assessment Project (IASAP) lunched to assess the health and environmental implications of the dumping. 1 tab

  18. Interactive reliability analysis project. FY 80 progress report

    International Nuclear Information System (INIS)

    Rasmuson, D.M.; Shepherd, J.C.

    1981-03-01

    This report summarizes the progress to date in the interactive reliability analysis project. Purpose is to develop and demonstrate a reliability and safety technique that can be incorporated early in the design process. Details are illustrated in a simple example of a reactor safety system

  19. Progress in Aging Epidemiology in Japan: The JAGES Project

    Directory of Open Access Journals (Sweden)

    Katsunori Kondo

    2016-07-01

    Full Text Available Aging is a prominent topic in global health. The purpose of this report is to document progress in two of our research projects in Japan, which currently is the most aged society in the world. The Japan Gerontological Evaluation Study (JAGES is one of the largest nation-wide research projects on aging, with more than 100 000 participants in 2010 and 2013. One of the notable findings is that community participation is a significant determinant of older people’s health. We have also made progress in the development of the JAGES Health Equity Assessment and Response Tools (HEART, which is a management tool for developing age-friendly cities. This progress suggests that community perspective and management of health promotion in the communities are valuable and require further research.

  20. The Qatar genome project: translation of whole-genome sequencing into clinical practice.

    Science.gov (United States)

    Zayed, Hatem

    2016-10-01

    Qatar Genome Project was launched in 2013 with the intent to sequence the genome of each Qatari citizen in an effort to protect Qataris from the high rate of indigenous genetic diseases by allowing the mapping of disease-causing variants/rare variants and establishing a Qatari reference genome. Indeed, this project is expected to have numerous global benefits because the elevated homogeneity of the Qatari population, that will make Qatar an excellent genetic laboratory that will generate a wealth of data that will allow us to make sense of the genotype-phenotype correlations of many diseases, especially the complex multifactorial diseases, and will pave the way for changing the traditional medical practice of looking first at the phenotype rather than the genotype. © 2016 John Wiley & Sons Ltd.

  1. The African Genome Variation Project shapes medical genetics in Africa

    Science.gov (United States)

    Gurdasani, Deepti; Carstensen, Tommy; Tekola-Ayele, Fasil; Pagani, Luca; Tachmazidou, Ioanna; Hatzikotoulas, Konstantinos; Karthikeyan, Savita; Iles, Louise; Pollard, Martin O.; Choudhury, Ananyo; Ritchie, Graham R. S.; Xue, Yali; Asimit, Jennifer; Nsubuga, Rebecca N.; Young, Elizabeth H.; Pomilla, Cristina; Kivinen, Katja; Rockett, Kirk; Kamali, Anatoli; Doumatey, Ayo P.; Asiki, Gershim; Seeley, Janet; Sisay-Joof, Fatoumatta; Jallow, Muminatou; Tollman, Stephen; Mekonnen, Ephrem; Ekong, Rosemary; Oljira, Tamiru; Bradman, Neil; Bojang, Kalifa; Ramsay, Michele; Adeyemo, Adebowale; Bekele, Endashaw; Motala, Ayesha; Norris, Shane A.; Pirie, Fraser; Kaleebu, Pontiano; Kwiatkowski, Dominic; Tyler-Smith, Chris; Rotimi, Charles; Zeggini, Eleftheria; Sandhu, Manjinder S.

    2014-01-01

    Given the importance of Africa to studies of human origins and disease susceptibility, detailed characterisation of African genetic diversity is needed. The African Genome Variation Project (AGVP) provides a resource to help design, implement and interpret genomic studies in sub-Saharan Africa (SSA) and worldwide. The AGVP represents dense genotypes from 1,481 and whole genome sequences (WGS) from 320 individuals across SSA. Using this resource, we find novel evidence of complex, regionally distinct hunter-gatherer and Eurasian admixture across SSA. We identify new loci under selection, including for malaria and hypertension. We show that modern imputation panels can identify association signals at highly differentiated loci across populations in SSA. Using WGS, we show further improvement in imputation accuracy supporting efforts for large-scale sequencing of diverse African haplotypes. Finally, we present an efficient genotype array design capturing common genetic variation in Africa, showing for the first time that such designs are feasible. PMID:25470054

  2. nGASP - the nematode genome annotation assessment project

    Energy Technology Data Exchange (ETDEWEB)

    Coghlan, A; Fiedler, T J; McKay, S J; Flicek, P; Harris, T W; Blasiar, D; Allen, J; Stein, L D

    2008-12-19

    While the C. elegans genome is extensively annotated, relatively little information is available for other Caenorhabditis species. The nematode genome annotation assessment project (nGASP) was launched to objectively assess the accuracy of protein-coding gene prediction software in C. elegans, and to apply this knowledge to the annotation of the genomes of four additional Caenorhabditis species and other nematodes. Seventeen groups worldwide participated in nGASP, and submitted 47 prediction sets for 10 Mb of the C. elegans genome. Predictions were compared to reference gene sets consisting of confirmed or manually curated gene models from WormBase. The most accurate gene-finders were 'combiner' algorithms, which made use of transcript- and protein-alignments and multi-genome alignments, as well as gene predictions from other gene-finders. Gene-finders that used alignments of ESTs, mRNAs and proteins came in second place. There was a tie for third place between gene-finders that used multi-genome alignments and ab initio gene-finders. The median gene level sensitivity of combiners was 78% and their specificity was 42%, which is nearly the same accuracy as reported for combiners in the human genome. C. elegans genes with exons of unusual hexamer content, as well as those with many exons, short exons, long introns, a weak translation start signal, weak splice sites, or poorly conserved orthologs were the most challenging for gene-finders. While the C. elegans genome is extensively annotated, relatively little information is available for other Caenorhabditis species. The nematode genome annotation assessment project (nGASP) was launched to objectively assess the accuracy of protein-coding gene prediction software in C. elegans, and to apply this knowledge to the annotation of the genomes of four additional Caenorhabditis species and other nematodes. Seventeen groups worldwide participated in nGASP, and submitted 47 prediction sets for 10 Mb of the C

  3. The Human Genome Diversity (HGD) Project. Summary document

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-12-31

    In 1991 a group of human geneticists and molecular biologists proposed to the scientific community that a world wide survey be undertaken of variation in the human genome. To aid their considerations, the committee therefore decided to hold a small series of international workshops to explore the major scientific issues involved. The intention was to define a framework for the project which could provide a basis for much wider and more detailed discussion and planning--it was recognized that the successful implementation of the proposed project, which has come to be known as the Human Genome Diversity (HGD) Project, would not only involve scientists but also various national and international non-scientific groups all of which should contribute to the project`s development. The international HGD workshop held in Sardinia in September 1993 was the last in the initial series of planning workshops. As such it not only explored new ground but also pulled together into a more coherent form much of the formal and informal discussion that had taken place in the preceding two years. This report presents the deliberations of the Sardinia workshop within a consideration of the overall development of the HGD Project to date.

  4. Imaging Genetics and Genomics in Psychiatry: A Critical Review of Progress and Potential.

    Science.gov (United States)

    Bogdan, Ryan; Salmeron, Betty Jo; Carey, Caitlin E; Agrawal, Arpana; Calhoun, Vince D; Garavan, Hugh; Hariri, Ahmad R; Heinz, Andreas; Hill, Matthew N; Holmes, Andrew; Kalin, Ned H; Goldman, David

    2017-08-01

    Imaging genetics and genomics research has begun to provide insight into the molecular and genetic architecture of neural phenotypes and the neural mechanisms through which genetic risk for psychopathology may emerge. As it approaches its third decade, imaging genetics is confronted by many challenges, including the proliferation of studies using small sample sizes and diverse designs, limited replication, problems with harmonization of neural phenotypes for meta-analysis, unclear mechanisms, and evidence that effect sizes may be more modest than originally posited, with increasing evidence of polygenicity. These concerns have encouraged the field to grow in many new directions, including the development of consortia and large-scale data collection projects and the use of novel methods (e.g., polygenic approaches, machine learning) that enhance the quality of imaging genetic studies but also introduce new challenges. We critically review progress in imaging genetics and offer suggestions and highlight potential pitfalls of novel approaches. Ultimately, the strength of imaging genetics and genomics lies in their translational and integrative potential with other research approaches (e.g., nonhuman animal models, psychiatric genetics, pharmacologic challenge) to elucidate brain-based pathways that give rise to the vast individual differences in behavior as well as risk for psychopathology. Copyright © 2017 Society of Biological Psychiatry. All rights reserved.

  5. Making Progress: The Use of Multiple Progress Reports to Enhance Advertising Students' Media Plan Term Projects

    Science.gov (United States)

    Kritz, Gary H.; Lozada, Hector R.; Long, Mary M.

    2007-01-01

    Since the AACSB mandates that students demonstrate effective oral and written communication skills, it is imperative that business professors do what is necessary to improve such skills. The authors investigate whether the use of using multiple progress reports in an Advertising class project improves the final product. The data results show that…

  6. The international Genome sample resource (IGSR): A worldwide collection of genome variation incorporating the 1000 Genomes Project data.

    Science.gov (United States)

    Clarke, Laura; Fairley, Susan; Zheng-Bradley, Xiangqun; Streeter, Ian; Perry, Emily; Lowy, Ernesto; Tassé, Anne-Marie; Flicek, Paul

    2017-01-04

    The International Genome Sample Resource (IGSR; http://www.internationalgenome.org) expands in data type and population diversity the resources from the 1000 Genomes Project. IGSR represents the largest open collection of human variation data and provides easy access to these resources. IGSR was established in 2015 to maintain and extend the 1000 Genomes Project data, which has been widely used as a reference set of human variation and by researchers developing analysis methods. IGSR has mapped all of the 1000 Genomes sequence to the newest human reference (GRCh38), and will release updated variant calls to ensure maximal usefulness of the existing data. IGSR is collecting new structural variation data on the 1000 Genomes samples from long read sequencing and other technologies, and will collect relevant functional data into a single comprehensive resource. IGSR is extending coverage with new populations sequenced by collaborating groups. Here, we present the new data and analysis that IGSR has made available. We have also introduced a new data portal that increases discoverability of our data-previously only browseable through our FTP site-by focusing on particular samples, populations or data sets of interest. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  7. Ceramic Technology Project semiannual progress report, October 1992--March 1993

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, D.R.

    1993-09-01

    This project was developed to meet the ceramic technology requirements of the OTS`s automotive technology programs. Although progress has been made in developing reliable structural ceramics, further work is needed to reduce cost. The work described in this report is organized according to the following work breakdown structure project elements: Materials and processing (monolithics [Si nitride, carbide], ceramic composites, thermal and wear coatings, joining, cost effective ceramic machining), materials design methodology (contact interfaces, new concepts), data base and life prediction (structural qualification, time-dependent behavior, environmental effects, fracture mechanics, nondestructive evaluation development), and technology transfer.

  8. Crystalline Repository Project. Technical progress report, October 1982-March 1983

    International Nuclear Information System (INIS)

    1985-01-01

    This document reports the progress being made periodically on the development of a geologic repository in crystalline rock for the permanent disposal of high-level nuclear waste. The reporting elements are arranged by the work breakdown structure so that related studies are presented together. The studies are reported by the Office of Crystalline Respository Development (OCRD), a prime contractor of the US Department of Energy Repository Project Office. The studies include work by other prime contractors and by subcontractors to OCRD

  9. Crowdfunding the Azolla fern genome project: a grassroots approach.

    Science.gov (United States)

    Li, Fay-Wei; Pryer, Kathleen M

    2014-01-01

    Much of science progresses within the tight boundaries of what is often seen as a "black box". Though familiar to funding agencies, researchers and the academic journals they publish in, it is an entity that outsiders rarely get to peek into. Crowdfunding is a novel means that allows the public to participate in, as well as to support and witness advancements in science. Here we describe our recent crowdfunding efforts to sequence the Azolla genome, a little fern with massive green potential. Crowdfunding is a worthy platform not only for obtaining seed money for exploratory research, but also for engaging directly with the general public as a rewarding form of outreach.

  10. 2012 U.S. Department of Energy: Joint Genome Institute: Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Gilbert, David [DOE JGI Public Affairs Manager

    2013-01-01

    The mission of the U.S. Department of Energy Joint Genome Institute (DOE JGI) is to serve the diverse scientific community as a user facility, enabling the application of large-scale genomics and analysis of plants, microbes, and communities of microbes to address the DOE mission goals in bioenergy and the environment. The DOE JGI's sequencing efforts fall under the Eukaryote Super Program, which includes the Plant and Fungal Genomics Programs; and the Prokaryote Super Program, which includes the Microbial Genomics and Metagenomics Programs. In 2012, several projects made news for their contributions to energy and environment research.

  11. The Genome of the Netherlands: design, and project goals

    Science.gov (United States)

    Boomsma, Dorret I; Wijmenga, Cisca; Slagboom, Eline P; Swertz, Morris A; Karssen, Lennart C; Abdellaoui, Abdel; Ye, Kai; Guryev, Victor; Vermaat, Martijn; van Dijk, Freerk; Francioli, Laurent C; Hottenga, Jouke Jan; Laros, Jeroen F J; Li, Qibin; Li, Yingrui; Cao, Hongzhi; Chen, Ruoyan; Du, Yuanping; Li, Ning; Cao, Sujie; van Setten, Jessica; Menelaou, Androniki; Pulit, Sara L; Hehir-Kwa, Jayne Y; Beekman, Marian; Elbers, Clara C; Byelas, Heorhiy; de Craen, Anton J M; Deelen, Patrick; Dijkstra, Martijn; den Dunnen, Johan T; de Knijff, Peter; Houwing-Duistermaat, Jeanine; Koval, Vyacheslav; Estrada, Karol; Hofman, Albert; Kanterakis, Alexandros; Enckevort, David van; Mai, Hailiang; Kattenberg, Mathijs; van Leeuwen, Elisabeth M; Neerincx, Pieter B T; Oostra, Ben; Rivadeneira, Fernanodo; Suchiman, Eka H D; Uitterlinden, Andre G; Willemsen, Gonneke; Wolffenbuttel, Bruce H; Wang, Jun; de Bakker, Paul I W; van Ommen, Gert-Jan; van Duijn, Cornelia M

    2014-01-01

    Within the Netherlands a national network of biobanks has been established (Biobanking and Biomolecular Research Infrastructure-Netherlands (BBMRI-NL)) as a national node of the European BBMRI. One of the aims of BBMRI-NL is to enrich biobanks with different types of molecular and phenotype data. Here, we describe the Genome of the Netherlands (GoNL), one of the projects within BBMRI-NL. GoNL is a whole-genome-sequencing project in a representative sample consisting of 250 trio-families from all provinces in the Netherlands, which aims to characterize DNA sequence variation in the Dutch population. The parent–offspring trios include adult individuals ranging in age from 19 to 87 years (mean=53 years; SD=16 years) from birth cohorts 1910–1994. Sequencing was done on blood-derived DNA from uncultured cells and accomplished coverage was 14–15x. The family-based design represents a unique resource to assess the frequency of regional variants, accurately reconstruct haplotypes by family-based phasing, characterize short indels and complex structural variants, and establish the rate of de novo mutational events. GoNL will also serve as a reference panel for imputation in the available genome-wide association studies in Dutch and other cohorts to refine association signals and uncover population-specific variants. GoNL will create a catalog of human genetic variation in this sample that is uniquely characterized with respect to micro-geographic location and a wide range of phenotypes. The resource will be made available to the research and medical community to guide the interpretation of sequencing projects. The present paper summarizes the global characteristics of the project. PMID:23714750

  12. MAKER2: an annotation pipeline and genome-database management tool for second-generation genome projects.

    Science.gov (United States)

    Holt, Carson; Yandell, Mark

    2011-12-22

    Second-generation sequencing technologies are precipitating major shifts with regards to what kinds of genomes are being sequenced and how they are annotated. While the first generation of genome projects focused on well-studied model organisms, many of today's projects involve exotic organisms whose genomes are largely terra incognita. This complicates their annotation, because unlike first-generation projects, there are no pre-existing 'gold-standard' gene-models with which to train gene-finders. Improvements in genome assembly and the wide availability of mRNA-seq data are also creating opportunities to update and re-annotate previously published genome annotations. Today's genome projects are thus in need of new genome annotation tools that can meet the challenges and opportunities presented by second-generation sequencing technologies. We present MAKER2, a genome annotation and data management tool designed for second-generation genome projects. MAKER2 is a multi-threaded, parallelized application that can process second-generation datasets of virtually any size. We show that MAKER2 can produce accurate annotations for novel genomes where training-data are limited, of low quality or even non-existent. MAKER2 also provides an easy means to use mRNA-seq data to improve annotation quality; and it can use these data to update legacy annotations, significantly improving their quality. We also show that MAKER2 can evaluate the quality of genome annotations, and identify and prioritize problematic annotations for manual review. MAKER2 is the first annotation engine specifically designed for second-generation genome projects. MAKER2 scales to datasets of any size, requires little in the way of training data, and can use mRNA-seq data to improve annotation quality. It can also update and manage legacy genome annotation datasets.

  13. Development and progress of the South African uranium enrichment project

    International Nuclear Information System (INIS)

    Roux, A.J.A.; Grant, W.L.; Barbour, R.A.; Loubser, R.S.; Wannenburg, J.J.

    1977-01-01

    The earlier development of the project is briefly reviewed, and some of the salient features of the South African process are touched upon. Development of the separation element in the last 18 months is discussed, as well as further work on the helikon cascade process. A brief description of the helikon cascade operation is given by means of diagrams. Because of time limitations, the complete helikon theory is not presented, but only some examples shown. Experimental work done to verify the helikon concept, as well as theoretical treatment, is presented. A brief report of the progress made on the experimental module of 6 t/a separative work capacity is given. This module, known as Mini-Z, is well advanced and details of its features and construction are shown. A short discussion of progress on the full-scale prototype module, known as Proto-Z, is next presented. The flexibility of such a design to fit a wide range of cascade sizes is considered, as well as cost implications of various approaches to design. Apart from progress on the development of the commercial plant, a brief review is given of the present state of the pilot plant at Valindaba. Some of the information obtained is mentioned. In conclusion, some information is given in regard to further planning and other work on the commercial plant at present being undertaken. Projected operation of the plant and some nuclear fuel service aspects are touched on

  14. The Personal Genome Project Canada: findings from whole genome sequences of the inaugural 56 participants.

    Science.gov (United States)

    Reuter, Miriam S; Walker, Susan; Thiruvahindrapuram, Bhooma; Whitney, Joe; Cohn, Iris; Sondheimer, Neal; Yuen, Ryan K C; Trost, Brett; Paton, Tara A; Pereira, Sergio L; Herbrick, Jo-Anne; Wintle, Richard F; Merico, Daniele; Howe, Jennifer; MacDonald, Jeffrey R; Lu, Chao; Nalpathamkalam, Thomas; Sung, Wilson W L; Wang, Zhuozhi; Patel, Rohan V; Pellecchia, Giovanna; Wei, John; Strug, Lisa J; Bell, Sherilyn; Kellam, Barbara; Mahtani, Melanie M; Bassett, Anne S; Bombard, Yvonne; Weksberg, Rosanna; Shuman, Cheryl; Cohn, Ronald D; Stavropoulos, Dimitri J; Bowdin, Sarah; Hildebrandt, Matthew R; Wei, Wei; Romm, Asli; Pasceri, Peter; Ellis, James; Ray, Peter; Meyn, M Stephen; Monfared, Nasim; Hosseini, S Mohsen; Joseph-George, Ann M; Keeley, Fred W; Cook, Ryan A; Fiume, Marc; Lee, Hin C; Marshall, Christian R; Davies, Jill; Hazell, Allison; Buchanan, Janet A; Szego, Michael J; Scherer, Stephen W

    2018-02-05

    The Personal Genome Project Canada is a comprehensive public data resource that integrates whole genome sequencing data and health information. We describe genomic variation identified in the initial recruitment cohort of 56 volunteers. Volunteers were screened for eligibility and provided informed consent for open data sharing. Using blood DNA, we performed whole genome sequencing and identified all possible classes of DNA variants. A genetic counsellor explained the implication of the results to each participant. Whole genome sequencing of the first 56 participants identified 207 662 805 sequence variants and 27 494 copy number variations. We analyzed a prioritized disease-associated data set ( n = 1606 variants) according to standardized guidelines, and interpreted 19 variants in 14 participants (25%) as having obvious health implications. Six of these variants (e.g., in BRCA1 or mosaic loss of an X chromosome) were pathogenic or likely pathogenic. Seven were risk factors for cancer, cardiovascular or neurobehavioural conditions. Four other variants - associated with cancer, cardiac or neurodegenerative phenotypes - remained of uncertain significance because of discrepancies among databases. We also identified a large structural chromosome aberration and a likely pathogenic mitochondrial variant. There were 172 recessive disease alleles (e.g., 5 individuals carried mutations for cystic fibrosis). Pharmacogenomics analyses revealed another 3.9 potentially relevant genotypes per individual. Our analyses identified a spectrum of genetic variants with potential health impact in 25% of participants. When also considering recessive alleles and variants with potential pharmacologic relevance, all 56 participants had medically relevant findings. Although access is mostly limited to research, whole genome sequencing can provide specific and novel information with the potential of major impact for health care. © 2018 Joule Inc. or its licensors.

  15. Documenting genomics: Applying archival theory to preserving the records of the Human Genome Project.

    Science.gov (United States)

    Shaw, Jennifer

    2016-02-01

    The Human Genome Archive Project (HGAP) aimed to preserve the documentary heritage of the UK's contribution to the Human Genome Project (HGP) by using archival theory to develop a suitable methodology for capturing the results of modern, collaborative science. After assessing past projects and different archival theories, the HGAP used an approach based on the theory of documentation strategy to try to capture the records of a scientific project that had an influence beyond the purely scientific sphere. The HGAP was an archival survey that ran for two years. It led to ninety scientists being contacted and has, so far, led to six collections being deposited in the Wellcome Library, with additional collections being deposited in other UK repositories. In applying documentation strategy the HGAP was attempting to move away from traditional archival approaches to science, which have generally focused on retired Nobel Prize winners. It has been partially successful in this aim, having managed to secure collections from people who are not 'big names', but who made an important contribution to the HGP. However, the attempt to redress the gender imbalance in scientific collections and to improve record-keeping in scientific organisations has continued to be difficult to achieve. Copyright © 2015 The Author. Published by Elsevier Ltd.. All rights reserved.

  16. Ceramic Technology Project semiannual progress report, April 1992--September 1992

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, D.R.

    1993-07-01

    This project was developed to meet the ceramic technology requirements of the DOE Office of Transportation Systems` automotive technology programs. Significant progress in fabricating ceramic components for DOE, NASA, and DOE advanced heat engine programs show that operation of ceramic parts in high-temperature engines is feasible; however, addition research is needed in materials and processing, design, and data base and life prediction before industry will have a sufficient technology base for producing reliable cost-effective ceramic engine components commercially. A 5-yr project plan was developed, with focus on structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines.

  17. Shippingport Station Decommissioning Project (SSDP). A progress report

    International Nuclear Information System (INIS)

    Mullee, G.R.; Usher, J.M.

    1986-01-01

    The Shippingport Atomic Power Station was shutdown in October, 1982 by the Plant Operator, Duquesne Light Company, for decommissioning by the US Department of Energy. The planning for decommissioning was completed in September, 1983. In September, 1984 operational responsibility for the station was transferred to the DOE's Decommissioning Operations Contractor - the General Electric Company (assisted by an integrated subcontractor, MK Ferguson Company). Significant accomplishments to date include the completion of all prerequisites for decommissioning, the removal of asbestos from plant systems, loading of irradiated reactor components into the reactor vessel for shipment, the commencement of electrical deactivations and the commencement of piping/component removal. Decontamination and waste processing are progressing in support of the project schedule. The reactor vessel will be shipped as one piece on a barge for burial at Hanford, Washington. The final release of the site is scheduled for April, 1990. A technology transfer program is being utilized to disseminate information about the project

  18. Relationship between Deleterious Variation, Genomic Autozygosity, and Disease Risk: Insights from The 1000 Genomes Project.

    Science.gov (United States)

    Pemberton, Trevor J; Szpiech, Zachary A

    2018-04-05

    Genomic regions of autozygosity (ROAs) represent segments of individual genomes that are homozygous for haplotypes inherited identical-by-descent (IBD) from a common ancestor. ROAs are nonuniformly distributed across the genome, and increased ROA levels are a reported risk factor for numerous complex diseases. Previously, we hypothesized that long ROAs are enriched for deleterious homozygotes as a result of young haplotypes with recent deleterious mutations-relatively untouched by purifying selection-being paired IBD as a consequence of recent parental relatedness, a pattern supported by ROA and whole-exome sequence data on 27 individuals. Here, we significantly bolster support for our hypothesis and expand upon our original analyses using ROA and whole-genome sequence data on 2,436 individuals from The 1000 Genomes Project. Considering CADD deleteriousness scores, we reaffirm our previous observation that long ROAs are enriched for damaging homozygotes worldwide. We show that strongly damaging homozygotes experience greater enrichment than weaker damaging homozygotes, while overall enrichment varies appreciably among populations. Mendelian disease genes and those encoding FDA-approved drug targets have significantly increased rates of gain in damaging homozygotes with increasing ROA coverage relative to all other genes. In genes implicated in eight complex phenotypes for which ROA levels have been identified as a risk factor, rates of gain in damaging homozygotes vary across phenotypes and populations but frequently differ significantly from non-disease genes. These findings highlight the potential confounding effects of population background in the assessment of associations between ROA levels and complex disease risk, which might underlie reported inconsistencies in ROA-phenotype associations. Copyright © 2018 American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

  19. Gene disruptions using P transposable elements: an integral component of the Drosophila genome project.

    OpenAIRE

    Spradling, A C; Stern, D M; Kiss, I; Roote, J; Laverty, T; Rubin, G M

    1995-01-01

    Biologists require genetic as well as molecular tools to decipher genomic information and ultimately to understand gene function. The Berkeley Drosophila Genome Project is addressing these needs with a massive gene disruption project that uses individual, genetically engineered P transposable elements to target open reading frames throughout the Drosophila genome. DNA flanking the insertions is sequenced, thereby placing an extensive series of genetic markers on the physical genomic map and a...

  20. Considerations for creating and annotating the budding yeast Genome Map at SGD: a progress report.

    Science.gov (United States)

    Chan, Esther T; Cherry, J Michael

    2012-01-01

    The Saccharomyces Genome Database (SGD) is compiling and annotating a comprehensive catalogue of functional sequence elements identified in the budding yeast genome. Recent advances in deep sequencing technologies have enabled for example, global analyses of transcription profiling and assembly of maps of transcription factor occupancy and higher order chromatin organization, at nucleotide level resolution. With this growing influx of published genome-scale data, come new challenges for their storage, display, analysis and integration. Here, we describe SGD's progress in the creation of a consolidated resource for genome sequence elements in the budding yeast, the considerations taken in its design and the lessons learned thus far. The data within this collection can be accessed at http://browse.yeastgenome.org and downloaded from http://downloads.yeastgenome.org. DATABASE URL: http://www.yeastgenome.org.

  1. Progress on the Hanford K basins spent nuclear fuel project

    International Nuclear Information System (INIS)

    Culley, G.E.; Fulton, J.C.; Gerber, E.W.

    1996-01-01

    This paper highlights progress made during the last year toward removing the Department of Energy's (DOE) approximately, 2,100 metric tons of metallic spent nuclear fuel from the two outdated K Basins at the Hanford Site and placing it in safe, economical interim dry storage. In the past year, the Spent Nuclear Fuel (SNF) Project has engaged in an evolutionary process involving the customer, regulatory bodies, and the public that has resulted in a quicker, cheaper, and safer strategy for accomplishing that goal. Development and implementation of the Integrated Process Strategy for K Basins Fuel is as much a case study of modern project and business management within the regulatory system as it is a technical achievement. A year ago, the SNF Project developed the K Basins Path Forward that, beginning in December 1998, would move the spent nuclear fuel currently stored in the K Basins to a new Staging and Storage Facility by December 2000. The second stage of this $960 million two-stage plan would complete the project by conditioning the metallic fuel and placing it in interim dry storage by 2006. In accepting this plan, the DOE established goals that the fuel removal schedule be accelerated by a year, that fuel conditioning be closely coupled with fuel removal, and that the cost be reduced by at least $300 million. The SNF Project conducted coordinated engineering and technology studies over a three-month period that established the technical framework needed to design and construct facilities, and implement processes compatible with these goals. The result was the Integrated Process Strategy for K Basins Fuel. This strategy accomplishes the goals set forth by the DOE by beginning fuel removal a year earlier in December 1997, completing it by December 1999, beginning conditioning within six months of starting fuel removal, and accomplishes it for $340 million less than the previous Path Forward plan

  2. First progress report on the Japan Endoscopy Database project.

    Science.gov (United States)

    Kodashima, Shinya; Tanaka, Kiyohito; Matsuda, Koji; Fujishiro, Mitsuhiro; Saito, Yutaka; Ohtsuka, Kazuo; Oda, Ichiro; Katada, Chikatoshi; Kato, Masayuki; Kida, Mitsuhiro; Kobayashi, Kiyonori; Hoteya, Shu; Horimatsu, Takahiro; Matsuda, Takahisa; Muto, Manabu; Yamamoto, Hironori; Ryozawa, Shomei; Iwakiri, Ryuichi; Kutsumi, Hiromu; Miyata, Hiroaki; Kato, Mototsugu; Haruma, Ken; Fujimoto, Kazuma; Uemura, Naomi; Kaminishi, Michio; Tajiri, Hisao

    2018-01-01

    The Japan Endoscopy Database (JED) Project was started to develop the world's largest endoscopic database, capture the actual performance of endoscopic practice, and standardize the terminology and fundamental items needed for a clinical and research registry. This paper presents a progress report on the first phase of this project undertaken at eight endoscopic centers in Japan. The list of data items to be collected was drafted by the MSED-J (Minimal Standard Endoscopic Database) subcommittee. These items were aggregated offline by integrating data from two endoscopic filing systems between July 2015 and December 2015. The study population included all patients who underwent esophagogastroduodenoscopy or colonoscopy at all eight centers, patients who underwent enteroscopy at five of the eight centers, and patients who underwent endoscopic retrograde cholangiopancreatography (ERCP) at four of the eight centers. Data collected in this phase included 61 070 endoscopic procedures, of which 40 475 were esophagogastroduodenoscopies, 215 were enteroscopies, 19 204 were colonoscopies, and 1176 were ERCPs. Frequencies of complications were 0.68% for esophagogastroduodenoscopy, 0% for enteroscopy, 0.43% for colonoscopy, and 13.34% for ERCP. In addition, we obtained various data including Helicobacter pylori infection status, past history of endoscopy in patients who underwent enteroscopy or colonoscopy, and degree of difficulty of ERCP, although the frequencies of reporting were sometimes low, with some items <20%. Results of the first phase suggest that the JED project can provide vast quantities of useful data about endoscopic procedures. © 2017 Japan Gastroenterological Endoscopy Society.

  3. A simulation of 'schedule-cost' progress monitoring system in nuclear power project management

    International Nuclear Information System (INIS)

    Song Haitao; Huang Zhongping; Zhang Zemin; Wang Zikai

    2010-01-01

    The objective of project management is to find the optimal balance between progress and cost according to the project requirements. Traditional method always manages progress and cost separately. However, domestic and international experience indicated that the interactions between these two factors are crucial in the project implementation. Modern project managers have to manage and maintain a 'Progress - Cost' joint control framework. Such a model is applied into a sub-project of a nuclear power project using Simulink in this paper. It helps to identify and correct the deviations of the project. Earned Value Management is used by the project manager to quantify the cost of the project and progress of implementation. The budget plan value, actual value, earned value are three important parameters to measure cost and progress of the project. The experimental results illustrated that the method gives a more comprehensive performance evaluation of the project. (authors)

  4. Cloning, production, and purification of proteins for a medium-scale structural genomics project.

    Science.gov (United States)

    Quevillon-Cheruel, Sophie; Collinet, Bruno; Trésaugues, Lionel; Minard, Philippe; Henckes, Gilles; Aufrère, Robert; Blondeau, Karine; Zhou, Cong-Zhao; Liger, Dominique; Bettache, Nabila; Poupon, Anne; Aboulfath, Ilham; Leulliot, Nicolas; Janin, Joël; van Tilbeurgh, Herman

    2007-01-01

    The South-Paris Yeast Structural Genomics Pilot Project (http://www.genomics.eu.org) aims at systematically expressing, purifying, and determining the three-dimensional structures of Saccharomyces cerevisiae proteins. We have already cloned 240 yeast open reading frames in the Escherichia coli pET system. Eighty-two percent of the targets can be expressed in E. coli, and 61% yield soluble protein. We have currently purified 58 proteins. Twelve X-ray structures have been solved, six are in progress, and six other proteins gave crystals. In this chapter, we present the general experimental flowchart applied for this project. One of the main difficulties encountered in this pilot project was the low solubility of a great number of target proteins. We have developed parallel strategies to recover these proteins from inclusion bodies, including refolding, coexpression with chaperones, and an in vitro expression system. A limited proteolysis protocol, developed to localize flexible regions in proteins that could hinder crystallization, is also described.

  5. Progress report on recommendations of the Flaring Project Team

    International Nuclear Information System (INIS)

    Macken, C.

    1999-01-01

    Part of the mandate of the Clean Air Strategic Alliance (CASA) is to share decision-making responsibility for air quality management with the government of Alberta, through the ministries of Environmental Protection, Energy, and Health, and the Alberta Energy and Utilities Board (EUB). CASA's vision for air quality in Alberta is that 'the air will be odourless, tasteless, look clear, and have no measurable short- or long-term adverse effects on people, animals, or the environment'. In 1997, CASA approved the establishment of the Flaring Project Team in response to public concern about potential and observed impacts associated with flaring of solution gas. Members of that team established a framework for the management of solution gas flaring. Their long-term goal is to eliminate routine flaring of solution gas. The Project Team assessed existing information on solution gas flaring, including technologies, efficiencies, emissions and impacts. Alternative technologies were also reviewed along with biological and health effects of solution gas flaring. A list of data gaps and research needs was compiled in order to help with the development of the Team's recommendations. The Team's final report was delivered in June 1998. It was recommended that the following policy objective hierarchy be used to guide decisions related to routine solution gas flaring: (1) eliminate routine solution gas flaring, (2) reduce volumes of gas flared, and (3) improve the efficiency of flares. By way of progress the Project Team was able to report that in March, 1999, the EUB issued a draft interim directive to address upstream petroleum industry flaring. The draft Directive incorporates the recommendations from the CASA Flaring Project Team with respect to management of solution gas flaring. In December 1998, changes to the royalty structure to encourage the productive use of flare gas have been announced by the Alberta Department of Energy and Alberta Environmental protection, thus

  6. Planning and progress of the WAGR decommissioning project

    International Nuclear Information System (INIS)

    Boorman, T.

    1988-01-01

    In the United Kingdom, the earliest production reactors, which will be decommissioned first, are of the Magnox type. The Windscale Advanced Gas-cooled Reactor, is however, sufficiently similar to make it a suitable prototype decommissioning project. The planning and progress so far is described. Special decommissioning equipment, including a remote dismantling machine, has been developed and a waste packaging building built on site. Its function is to enable all intermediate-level and low-level radioactive waste removed from the reactor vault by remote equipment to be packaged remotely into suitable containers. The work done on the WAGR decommissioning has shown that the dismantling of a power-producing reactor is feasible and can be accomplished using existing engineering techniques. (U.K.)

  7. The GenABEL Project for statistical genomics.

    Science.gov (United States)

    Karssen, Lennart C; van Duijn, Cornelia M; Aulchenko, Yurii S

    2016-01-01

    Development of free/libre open source software is usually done by a community of people with an interest in the tool. For scientific software, however, this is less often the case. Most scientific software is written by only a few authors, often a student working on a thesis. Once the paper describing the tool has been published, the tool is no longer developed further and is left to its own device. Here we describe the broad, multidisciplinary community we formed around a set of tools for statistical genomics. The GenABEL project for statistical omics actively promotes open interdisciplinary development of statistical methodology and its implementation in efficient and user-friendly software under an open source licence. The software tools developed withing the project collectively make up the GenABEL suite, which currently consists of eleven tools. The open framework of the project actively encourages involvement of the community in all stages, from formulation of methodological ideas to application of software to specific data sets. A web forum is used to channel user questions and discussions, further promoting the use of the GenABEL suite. Developer discussions take place on a dedicated mailing list, and development is further supported by robust development practices including use of public version control, code review and continuous integration. Use of this open science model attracts contributions from users and developers outside the "core team", facilitating agile statistical omics methodology development and fast dissemination.

  8. The human genome project and the Catholic Church (1)

    Science.gov (United States)

    Moraczewski, Albert S

    1991-12-01

    The Cathlic Church has not made any formal statements about the Human Genome Project as such. But the present Pope, John Paul II, has commented, albeit very briefly, on various aspects of genetic manipulation. Genetic interventions which are therapeutic (e.g. gene therapy), namely, directed to the correction or amelioration of a disorder are acceptable, in principle, provided they promote the personal well being of the individual being so treated. Genetic interventions which are not therapeutic for the specific individual involved but are experimental and directed primarily to improving humans as biological entities are of dubious moral probity, but are not necessarily to be totally rejected out of hand. To be morally acceptable such genetic intervention should meet certain conditions which include due respect for the given psychological nature of each individual human being. In addition, no harm should be inflicted on the process of human generation, and its fundamental design should not be altered. Any genetic manipulation which results in, or tends to, the creation of groups with different qualities such that there would result a fresh marginalization of these people must be avoided. It has been also suggested by a few that because the Son of God took on a human nature in Jesus Christ, one may not so alter the human genome that a new distinct species would be created....

  9. The UK Human Genome Mapping Project online computing service.

    Science.gov (United States)

    Rysavy, F R; Bishop, M J; Gibbs, G P; Williams, G W

    1992-04-01

    This paper presents an overview of computing and networking facilities developed by the Medical Research Council to provide online computing support to the Human Genome Mapping Project (HGMP) in the UK. The facility is connected to a number of other computing facilities in various centres of genetics and molecular biology research excellence, either directly via high-speed links or through national and international wide-area networks. The paper describes the design and implementation of the current system, a 'client/server' network of Sun, IBM, DEC and Apple servers, gateways and workstations. A short outline of online computing services currently delivered by this system to the UK human genetics research community is also provided. More information about the services and their availability could be obtained by a direct approach to the UK HGMP-RC.

  10. Progress with the NESC spinning cylinder project and other NESC projects

    International Nuclear Information System (INIS)

    Wintle, J.B.; Hurst, R.C.; Hemsworth, B.

    1995-01-01

    The first international project (NESC I) of the Network of Evaluating Steel Components is a spinning cylinder, pressurized thermal shock (PTS) experiment. The main objective of the project is to validate the non-destructive evaluation and structural mechanics procedures for PWR reactor pressure vessels under PTS conditions. Contributing organizations world-wide will participate in this blind trial which embraces all aspects of structural integrity assessment. This paper describes the progress of the project to date, covering cylinder manufacture and inspection, materials evaluation, structural analysis and test instrumentation. It emphasizes the importance of networking global expertise in a managed framework and of the partnership, co-operation and teamwork developed by the contributing organizations through the five Task Groups constituting the NESC I. Finally, five new initiatives for projects managed by the Network are currently under review and described in this paper

  11. Progress with the NESC spinning cylinder project and other NESC projects

    International Nuclear Information System (INIS)

    Wintle, J.B.; Hurst, R.C.; Hemsworth, B.

    1995-01-01

    The first international project (NESC I) of the Network for Evaluating Steel Components is a spinning cylinder, pressurized thermal shock (PTS) experiment. The main objective of the project is to validate the non-destructive evaluation and structural mechanics procedures for PWR reactor pressure vessels under PTS conditions. Contributing organizations world-wide will participate in this blind trial which embraces all aspects of structural integrity assessment. This paper describes the progress of the project to date, covering cylinder manufacture and inspection, materials evaluation, structural analysis and test instrumentation. It emphasises the importance of networking global expertise in a managed framework and of the partnership, co-operation and teamwork developed by the contributing organizations through the five Task Groups constituting NESC I. Finally, five new initiatives for projects managed by the Network are currently under review and described in this paper. (author). 2 refs, 6 figs

  12. The Midwifery Legacies Project: history, progress, and future directions.

    Science.gov (United States)

    Moore-Davis, Tonia L; McGee, Karen B; Moore, Elaine M; Paine, Lisa L

    2015-01-01

    The Midwifery Legacies Project, formerly known as the OnGoing Group, was founded as an annual greeting card outreach aimed at maintaining contact with midwives as they approached retirement and beyond. In 2009, the importance of documenting personal and professional stories of midwives arose out of a bequest by a midwife who was relatively unknown outside of the community she served. The result has been the evolution of a robust collection of stories, which are known as the 20th Century Midwife Story Collection. Between 2009 and 2014, more than 120 US midwives aged 65 years or older were interviewed by a midwife, a student midwife, or a professional filmmaker. Collectively, these midwives' stories offer an intimate snapshot of the social, political, and cultural influences that have shaped US midwifery during the past half century. Individually, the stories honor and recognize midwives' contributions to the profession and the women they have served. This article details the development, progress, and future directions of the Midwifery Legacies Project. © 2015 by the American College of Nurse-Midwives.

  13. Progress of the Hanford Bulk Vitrification Project ICVTM Testing Program

    International Nuclear Information System (INIS)

    Witwer, K.S.; Woolery, D.W.; Dysland, E.J.

    2006-01-01

    In June 2004, the Bulk Vitrification Project was initiated with the intent to engineer, construct and operate a full-scale bulk vitrification pilot-plant to treat low-activity tank waste from Hanford tank 241-S-109. The project, managed by CH2M HILL Hanford Group, Inc., and performed by AMEC Earth and Environmental, Inc. (AMEC), will develop and operate a full-scale demonstration facility to exhibit the effectiveness of the bulk vitrification process under actual operating conditions. Since project initiation, testing has been undertaken using crucible-scale, 1/6 linear (engineering) scale, and full-scale vitrification equipment. Crucible-scale testing, coupled with engineering-scale testing, helps establish process limitations of selected glass formulations. Full-scale testing provides critical design verification of the In Container Vitrification (ICV) TM process both prior to and during operation of the demonstration facility. Beginning in late 2004, several full-scale tests have been performed at AMEC's test site, located adjacent to the U.S. Department of Energy's Hanford Site, in Richland, WA. Early testing involved verification of melt startup methodology, followed by subsequent full-melt testing to validate critical design parameters and demonstrate the 'Bottom-Up, Feed While Melt' process. As testing has progressed, design improvements have been identified and incorporated into each successive test. Full scale testing at AMEC's test site is currently scheduled to complete in 2006, with continued full-scale operational testing at the demonstration facility on the Hanford Site starting in 2007. Additional engineering scale testing will validate recommended glass formulations that have been provided by the Pacific Northwest National Laboratory (PNNL). This testing is expected to continue through 2006. This paper discusses the progress of the full-scale and engineering scale testing performed to date. Crucible-scale testing, a critical step in developing

  14. The Epilepsy Phenome/Genome Project (EPGP) informatics platform.

    Science.gov (United States)

    Nesbitt, Gerry; McKenna, Kevin; Mays, Vickie; Carpenter, Alan; Miller, Kevin; Williams, Michael

    2013-04-01

    The Epilepsy Phenome/Genome Project (EPGP) is a large-scale, multi-institutional, collaborative network of 27 epilepsy centers throughout the U.S., Australia, and Argentina, with the objective of collecting detailed phenotypic and genetic data on a large number of epilepsy participants. The goals of EPGP are (1) to perform detailed phenotyping on 3750 participants with specific forms of non-acquired epilepsy and 1500 parents without epilepsy, (2) to obtain DNA samples on these individuals, and (3) to ultimately genotype the samples in order to discover novel genes that cause epilepsy. To carry out the project, a reliable and robust informatics platform was needed for standardized electronic data collection and storage, data quality review, and phenotypic analysis involving cases from multiple sites. EPGP developed its own suite of web-based informatics applications for participant tracking, electronic data collection (using electronic case report forms/surveys), data management, phenotypic data review and validation, specimen tracking, electroencephalograph and neuroimaging storage, and issue tracking. We implemented procedures to train and support end-users at each clinical site. Thus far, 3780 study participants have been enrolled and 20,957 web-based study activities have been completed using this informatics platform. Over 95% of respondents to an end-user satisfaction survey felt that the informatics platform was successful almost always or most of the time. The EPGP informatics platform has successfully and effectively allowed study management and efficient and reliable collection of phenotypic data. Our novel informatics platform met the requirements of a large, multicenter research project. The platform has had a high level of end-user acceptance by principal investigators and study coordinators, and can serve as a model for new tools to support future large scale, collaborative research projects collecting extensive phenotypic data. Copyright © 2012

  15. Genome-wide analysis of disease progression in age-related macular degeneration.

    Science.gov (United States)

    Yan, Qi; Ding, Ying; Liu, Yi; Sun, Tao; Fritsche, Lars G; Clemons, Traci; Ratnapriya, Rinki; Klein, Michael L; Cook, Richard J; Liu, Yu; Fan, Ruzong; Wei, Lai; Abecasis, Gonçalo R; Swaroop, Anand; Chew, Emily Y; Weeks, Daniel E; Chen, Wei

    2018-03-01

    Family- and population-based genetic studies have successfully identified multiple disease-susceptibility loci for Age-related macular degeneration (AMD), one of the first batch and most successful examples of genome-wide association study. However, most genetic studies to date have focused on case-control studies of late AMD (choroidal neovascularization or geographic atrophy). The genetic influences on disease progression are largely unexplored. We assembled unique resources to perform a genome-wide bivariate time-to-event analysis to test for association of time-to-late-AMD with ∼9 million variants on 2721 Caucasians from a large multi-center randomized clinical trial, the Age-Related Eye Disease Study. To our knowledge, this is the first genome-wide association study of disease progression (bivariate survival outcome) in AMD genetic studies, thus providing novel insights to AMD genetics. We used a robust Cox proportional hazards model to appropriately account for between-eye correlation when analyzing the progression time in the two eyes of each participant. We identified four previously reported susceptibility loci showing genome-wide significant association with AMD progression: ARMS2-HTRA1 (P = 8.1 × 10-43), CFH (P = 3.5 × 10-37), C2-CFB-SKIV2L (P = 8.1 × 10-10) and C3 (P = 1.2 × 10-9). Furthermore, we detected association of rs58978565 near TNR (P = 2.3 × 10-8), rs28368872 near ATF7IP2 (P = 2.9 × 10-8) and rs142450006 near MMP9 (P = 0.0006) with progression to choroidal neovascularization but not geographic atrophy. Secondary analysis limited to 34 reported risk variants revealed that LIPC and CTRB2-CTRB1 were also associated with AMD progression (P < 0.0015). Our genome-wide analysis thus expands the genetics in both development and progression of AMD and should assist in early identification of high risk individuals.

  16. Ethical considerations of research policy for personal genome analysis: the approach of the Genome Science Project in Japan.

    Science.gov (United States)

    Minari, Jusaku; Shirai, Tetsuya; Kato, Kazuto

    2014-12-01

    As evidenced by high-throughput sequencers, genomic technologies have recently undergone radical advances. These technologies enable comprehensive sequencing of personal genomes considerably more efficiently and less expensively than heretofore. These developments present a challenge to the conventional framework of biomedical ethics; under these changing circumstances, each research project has to develop a pragmatic research policy. Based on the experience with a new large-scale project-the Genome Science Project-this article presents a novel approach to conducting a specific policy for personal genome research in the Japanese context. In creating an original informed-consent form template for the project, we present a two-tiered process: making the draft of the template following an analysis of national and international policies; refining the draft template in conjunction with genome project researchers for practical application. Through practical use of the template, we have gained valuable experience in addressing challenges in the ethical review process, such as the importance of sharing details of the latest developments in genomics with members of research ethics committees. We discuss certain limitations of the conventional concept of informed consent and its governance system and suggest the potential of an alternative process using information technology.

  17. Human Genome Teacher Networking Project, Final Report, April 1, 1992 - March 31, 1998

    Energy Technology Data Exchange (ETDEWEB)

    Collins, Debra

    1999-10-01

    Project to provide education regarding ethical legal and social implications of Human Genome Project to high school science teachers through two consecutive summer workshops, in class activities, and peer teaching workshops.

  18. The lawful uses of knowledge from the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Grad, F.P.

    1994-04-15

    Part I of this study deals with the right to know or not to know personal genetic information, and examines available legal protections of the right of privacy and the adverse effect of the disclosure of genetic information both on employment and insurance interests and on self esteem and protection of personal integrity. The study examines the rationale for the legal protection of privacy as the protection of a public interest. It examines the very limited protections currently available for privacy interests, including genetic privacy interests, and concludes that there is a need for broader, more far-reaching legal protections. The second part of the study is based on the assumption that as major a project as the Human Genome Project, spending billions of dollars on science which is health related, will indeed be applied for preventive and therapeutic public health purposes, as it has been in the past. It also addresses the recurring fear that public health initiatives in the genetic area must evolve a new eugenic agenda, that we must not repeat the miserable discriminatory experiences of the past.

  19. Enabling a Community to Dissect an Organism: Overview of the Neurospora Functional Genomics Project

    OpenAIRE

    Dunlap, Jay C.; Borkovich, Katherine A.; Henn, Matthew R.; Turner, Gloria E.; Sachs, Matthew S.; Glass, N. Louise; McCluskey, Kevin; Plamann, Michael; Galagan, James E.; Birren, Bruce W.; Weiss, Richard L.; Townsend, Jeffrey P.; Loros, Jennifer J.; Nelson, Mary Anne; Lambreghts, Randy

    2007-01-01

    A consortium of investigators is engaged in a functional genomics project centered on the filamentous fungus Neurospora, with an eye to opening up the functional genomic analysis of all the filamentous fungi. The overall goal of the four interdependent projects in this effort is to acccomplish functional genomics, annotation, and expression analyses of Neurospora crassa, a filamentous fungus that is an established model for the assemblage of over 250,000 species of nonyeast fungi. Building fr...

  20. The Genomes On Line Database (GOLD) in 2009: status of genomic and metagenomic projects and their associated metadata

    Science.gov (United States)

    Liolios, Konstantinos; Chen, I-Min A.; Mavromatis, Konstantinos; Tavernarakis, Nektarios; Hugenholtz, Philip; Markowitz, Victor M.; Kyrpides, Nikos C.

    2010-01-01

    The Genomes On Line Database (GOLD) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete and ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables and a search page. As of September 2009, GOLD contains information for more than 5800 sequencing projects, of which 1100 have been completed and their sequence data deposited in a public repository. GOLD continues to expand, moving toward the goal of providing the most comprehensive repository of metadata information related to the projects and their organisms/environments in accordance with the Minimum Information about a (Meta)Genome Sequence (MIGS/MIMS) specification. GOLD is available at: http://www.genomesonline.org and has a mirror site at the Institute of Molecular Biology and Biotechnology, Crete, Greece, at: http://gold.imbb.forth.gr/ PMID:19914934

  1. Bat biology, genomes, and the Bat1K project

    DEFF Research Database (Denmark)

    Teeling, Emma C; Vernes, Sonja C; Dávalos, Liliana M

    2018-01-01

    and endangered. Here we announce Bat1K, an initiative to sequence the genomes of all living bat species (n∼1,300) to chromosome-level assembly. The Bat1K genome consortium unites bat biologists (>148 members as of writing), computational scientists, conservation organizations, genome technologists, and any...

  2. Progress report: Bangladesh [CSC Project on Management of Water Hyacinth

    International Nuclear Information System (INIS)

    Haider, S.Z.

    1981-01-01

    The CSC Project on Management of Water Hyacinth in Bangladesh was started in only April 1981. The present report from Bangladesh is based on the interim progress reports, submitted by the project coordinators of the organizations concerned, for the work during the short intervening period to 4th June 1981. It is obvious that the report deals mainly with the preliminary preparations and observations on the various aspects of the investigations. The research components assigned to various organisations in Bangladesh are: Bangladesh University of Engineering and Technology; - Growth rate and biogas production; Housing and Building Research Institute; - Paper and board, and biogas; Bangladesh Council of Scientific and Industrial Research - Protein, enzymes and ammoacids; Dacca University - Pollution removal and studies of biomass production. Bangladesh is a flat deltaic plain formed from old and new alluvium. The areas of old alluvium are located above the general level, while the vast areas of new alluvium are situated m the flood plain of the modern rivers. The whole country is intersected by a network of rivers; the areas comprising the alluvial plains are very large. The river bank levees are high and the land gradually slopes inland away from the levees. The tract of land between two rivers are shaped like a furrow and in most cases form elongated 'beels' or swamps which are ideal place for the growth and propagation of aquatic plants particularly water hyacinth along with other aquatic organisms, both of plant and animal origin due to eutrophication. It is in the shallow swamps and derelict ponds that luxuriant growth and proliferation of water hyacinth is generally observed in Bangladesh. However, there is a feeling that much of the water hyacinth population in the open countryside was depleted during the severe drought which prevailed during the summer of 1979. But Bangladesh has the ideal water and climatic environment for conditions of rapid growth and spread

  3. The 150 ns detector project: progress with small detectors

    International Nuclear Information System (INIS)

    Warburton, W.K.; Russell, S.R.; Kleinfelder, Stuart A.; Segal, Julie

    1994-01-01

    This project's long term goal is to develop a pixel area detector capable of 6 MHz frame rates (150 ns/frame). Our milestones toward this goal are: a single pixel, 1x256 1D and 8x8 2D detectors, 256x256 2D detectors and, finally, 1024x1024 2D detectors. The design strategy is to supply a complete electronics chain (resetting preamp, selectable gain amplifier, analog-to-digital converter (ADC), and memory) for each pixel. In the final detectors these will all be custom integrated circuits. The front end preamplifiers are being integrated first, since their design and performance are both the most unusual and also critical to the project's success. Similarly, our early work is also concentrating on devising and perfecting detector structures which are thick enough (1 mm) to absorb over 99% of the incident X-rays in the energy range of interest. In this paper we discuss our progress toward the 1x256 1D and 8x8 2D detectors. We have fabricated sample detectors at Stanford's Center for Integrated Systems and are preparing both to test them individually and to wirebond them to the preamplifier samples to produce our first working small 1D and 2D detectors. We will describe our solutions to the design problems associated with collecting charge in less than 30 ns from 1 mm thick pixels in high resistivity silicon. We have constructed and tested the front end of our preamplifier design using a commercial 1.2 μm CMOS technology and are moving on to produce a few channels of the complete preamplifier, including a switchable gain stage and output stage. We will discuss both the preamplifier design and our initial test results. ((orig.))

  4. The 150 ns detector project: progress with small detectors

    Energy Technology Data Exchange (ETDEWEB)

    Warburton, W.K. (X-ray Instrumentation Associates, 2513 Charleston Rd, Ste 207, Mountain View, CA 94043 (United States)); Russell, S.R. (X-ray Instrumentation Associates, 2513 Charleston Rd, Ste 207, Mountain View, CA 94043 (United States)); Kleinfelder, Stuart A. (VLSI Physics, 19 Drury Lane, Berkeley, CA 94705 (United States)); Segal, Julie (Integrated Ckts Lab., Dept. of Electrical Engineering, Stanford University, Stanford, CA 94305 (United States))

    1994-09-01

    This project's long term goal is to develop a pixel area detector capable of 6 MHz frame rates (150 ns/frame). Our milestones toward this goal are: a single pixel, 1x256 1D and 8x8 2D detectors, 256x256 2D detectors and, finally, 1024x1024 2D detectors. The design strategy is to supply a complete electronics chain (resetting preamp, selectable gain amplifier, analog-to-digital converter (ADC), and memory) for each pixel. In the final detectors these will all be custom integrated circuits. The front end preamplifiers are being integrated first, since their design and performance are both the most unusual and also critical to the project's success. Similarly, our early work is also concentrating on devising and perfecting detector structures which are thick enough (1 mm) to absorb over 99% of the incident X-rays in the energy range of interest. In this paper we discuss our progress toward the 1x256 1D and 8x8 2D detectors. We have fabricated sample detectors at Stanford's Center for Integrated Systems and are preparing both to test them individually and to wirebond them to the preamplifier samples to produce our first working small 1D and 2D detectors. We will describe our solutions to the design problems associated with collecting charge in less than 30 ns from 1 mm thick pixels in high resistivity silicon. We have constructed and tested the front end of our preamplifier design using a commercial 1.2 [mu]m CMOS technology and are moving on to produce a few channels of the complete preamplifier, including a switchable gain stage and output stage. We will discuss both the preamplifier design and our initial test results. ((orig.))

  5. Contracts for field projects and supporting research on enhanced oil recovery. Progress review number 87

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-10-01

    Approximately 30 research projects are summarized in this report. Title of the project, contract number, company or university, award amount, principal investigators, objectives, and summary of technical progress are given for each project. Enhanced oil recovery projects include chemical flooding, gas displacement, and thermal recovery. Most of the research projects though are related to geoscience technology and reservoir characterization.

  6. Genomic evolution of Staphylococcus aureus isolates colonizing the nares and progressing to bacteremia.

    Directory of Open Access Journals (Sweden)

    Jeanne B Benoit

    Full Text Available Nasal colonization by Staphylococcus aureus is a key risk factor for bacteremia. The objective of this study is to identify genomic modifications occurring in nasal carriage strains of S. aureus as they progress to bacteremia in a cohort of hospitalized patients.Eight patients with S. aureus bacteremia were identified. Genomic sequences of the bloodstream isolates were compared with 57 nasal isolates collected longitudinally prior to the occurrence of bacteremia, which covered a timespan of up to 326 days before bacteremia.Within each subject, nasal colonizing strains were closely related to bacteremia strains. Within a subject, the number of single nucleotide polymorphisms (SNPs observed between time points was greater than within a single time point. Co-colonization and strain replacement were observed in one case. In all cases colonization progressed to bacteremia without addition of new virulence genes. In one case, a mutation in the accessory gene regulator gene caused abrogation of agr function.S. aureus evolves in the human nares at a variable rate. Progression of S. aureus nasal colonization to nosocomial infection is seldom associated with acquisition of new virulence determinants. Mutation in the agr gene with abrogation of function was associated with progression to bacteremia in one case.

  7. The MICROBE Project, A Report from the Interagency Working Group on Microbial Genomics

    Science.gov (United States)

    2001-01-01

    functional genomics tools (gene chips, technologies, etc.), comparative genomics, proteomics tools, novel culture techniques, in situ analyses, and...interested in supporting microarray/chip development for gene expression analysis for agricultural microbes, bioinformatics, and proteomics , and the...including one fungus ) in various stages of progress. The closely integrated Natural and Accelerated Bioremediation Research Program in the Office of

  8. Sequencing and characterizing the genome of Estrella lausannensis as an undergraduate project: training students and biological insights

    Directory of Open Access Journals (Sweden)

    Claire eBertelli

    2015-02-01

    Full Text Available With the widespread availability of high-throughput sequencing technologies, sequencing projects have become pervasive in the molecular life sciences. The huge bulk of data generated daily must be analyzed further by biologists with skills in bioinformatics and by embedded bioinformaticians, i.e., bioinformaticians integrated in wet lab research groups. Thus, students interested in molecular life sciences must be trained in the main steps of genomics: sequencing, assembly, annotation and analysis. To reach that goal, a practical course has been set up for master students at the University of Lausanne: the Sequence a genome class. At the beginning of the academic year, a few bacterial species whose genome is unknown are provided to the students, who sequence and assemble the genome(s and perform manual annotation. Here, we report the progress of the first class from September 2010 to June 2011 and the results obtained by seven master students who specifically assembled and annotated the genome of Estrella lausannensis, an obligate intracellular bacterium related to Chlamydia. The draft genome of Estrella is composed of 29 scaffolds encompassing 2,819,825 bp that encode for 2,233 putative proteins. Estrella also possesses a 9,136 bp plasmid that encodes for 14 genes, among which we found an integrase and a toxin/antitoxin module. Like all other members of the Chlamydiales order, Estrella possesses a highly conserved type III secretion system, considered as a key virulence factor. The annotation of the Estrella genome also allowed the characterization of the metabolic abilities of this strictly intracellular bacterium. Altogether, the students provided the scientific community with the Estrella genome sequence and a preliminary understanding of the biology of this recently-discovered bacterial genus, while learning to use cutting-edge technologies for sequencing and to perform bioinformatics analyses.

  9. The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata

    Science.gov (United States)

    Pagani, Ioanna; Liolios, Konstantinos; Jansson, Jakob; Chen, I-Min A.; Smirnova, Tatyana; Nosrat, Bahador; Markowitz, Victor M.; Kyrpides, Nikos C.

    2012-01-01

    The Genomes OnLine Database (GOLD, http://www.genomesonline.org/) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete and ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables and a search page. As of September 2011, GOLD, now on version 4.0, contains information for 11 472 sequencing projects, of which 2907 have been completed and their sequence data has been deposited in a public repository. Out of these complete projects, 1918 are finished and 989 are permanent drafts. Moreover, GOLD contains information for 340 metagenome studies associated with 1927 metagenome samples. GOLD continues to expand, moving toward the goal of providing the most comprehensive repository of metadata information related to the projects and their organisms/environments in accordance with the Minimum Information about any (x) Sequence specification and beyond. PMID:22135293

  10. Rhipicephalus (Boophilus) microplus strain Deutsch, whole genome shotgun sequencing project first submission of genome sequence

    Science.gov (United States)

    The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence difficult. Cot filtration/selection techniques were used to reduce the repetitive fraction of the tick genome and enrich for the fraction of DNA with gene-containing regions. The Cot-selected ...

  11. Drosophila Sld5 is essential for normal cell cycle progression and maintenance of genomic integrity

    Energy Technology Data Exchange (ETDEWEB)

    Gouge, Catherine A. [Department of Biology, East Carolina University East Carolina University, Greenville, NC 27858 (United States); Christensen, Tim W., E-mail: christensent@ecu.edu [Department of Biology, East Carolina University East Carolina University, Greenville, NC 27858 (United States)

    2010-09-10

    Research highlights: {yields} Drosophila Sld5 interacts with Psf1, PPsf2, and Mcm10. {yields} Haploinsufficiency of Sld5 leads to M-phase delay and genomic instability. {yields} Sld5 is also required for normal S phase progression. -- Abstract: Essential for the normal functioning of a cell is the maintenance of genomic integrity. Failure in this process is often catastrophic for the organism, leading to cell death or mis-proliferation. Central to genomic integrity is the faithful replication of DNA during S phase. The GINS complex has recently come to light as a critical player in DNA replication through stabilization of MCM2-7 and Cdc45 as a member of the CMG complex which is likely responsible for the processivity of helicase activity during S phase. The GINS complex is made up of 4 members in a 1:1:1:1 ratio: Psf1, Psf2, Psf3, And Sld5. Here we present the first analysis of the function of the Sld5 subunit in a multicellular organism. We show that Drosophila Sld5 interacts with Psf1, Psf2, and Mcm10 and that mutations in Sld5 lead to M and S phase delays with chromosomes exhibiting hallmarks of genomic instability.

  12. HBV genome analysis in the progression of HBV related chronic liver disease

    Directory of Open Access Journals (Sweden)

    Ruksana Raihan

    2017-12-01

    Full Text Available Although HBV is a non-cytopathic virus, alteration of viral genome may also alter host immunity and may play a part in the pathogenesis LC and HCC. During the last decade, various studies have shown that mutations in the HBV genome may play a role in HCC pathogenesis. Here, we have analyzed HBV genome from patients with asymptomatic HBV carrier [ASC], chronic hepatitis B (CHB, cirrhosis of liver (LC, and hepatocellular carcinoma (HCC of Bangladeshi origin. A total of 225 patients tested positive for HBV with different stages of chronic HBV infection were enrolled in this study. The extent of liver damages were assayed by estimating serum levels of alanine aminotransferase (ALT, serum bilirubin and finally by abdominal ultrasonography and/or fine needle aspiration cytology. Wherever required, cancer marker like alpha fetoprotein (AFP was assessed. HBV genotype was evaluated by immunoassays and sequenced. A total of 25 patients were ASC, 135 were CHB and 65 were LC and HCC. Among ASC patients, 5, 7 and 13 belonged to HBV genotype A, C, and D, respectively. On the other hand, HBV genotype C was most prevalent in CHB patients (about 42%, followed by HBV genotype D (36%. About 69% patients with LC and HCC also had genotype C. Full genomic analysis of sera of patients with progressive liver damages (LC and HCC revealed mutations at HBeAg promoter regions in more than 80% patients. However, mutations in this region were mostly unseen in ASC and patients with less progressive liver diseases. HBV genotype was found quite different in Bangladeshi HBV patients which seem a mixture of Indian and Asia-Pacific region. This study also reveals that HBeAg promoter region mutation may have role in development of HBV related LC and HCC.

  13. Matching phenotypes to whole genomes: Lessons learned from four iterations of the personal genome project community challenges.

    Science.gov (United States)

    Cai, Binghuang; Li, Biao; Kiga, Nikki; Thusberg, Janita; Bergquist, Timothy; Chen, Yun-Ching; Niknafs, Noushin; Carter, Hannah; Tokheim, Collin; Beleva-Guthrie, Violeta; Douville, Christopher; Bhattacharya, Rohit; Yeo, Hui Ting Grace; Fan, Jean; Sengupta, Sohini; Kim, Dewey; Cline, Melissa; Turner, Tychele; Diekhans, Mark; Zaucha, Jan; Pal, Lipika R; Cao, Chen; Yu, Chen-Hsin; Yin, Yizhou; Carraro, Marco; Giollo, Manuel; Ferrari, Carlo; Leonardi, Emanuela; Tosatto, Silvio C E; Bobe, Jason; Ball, Madeleine; Hoskins, Roger A; Repo, Susanna; Church, George; Brenner, Steven E; Moult, John; Gough, Julian; Stanke, Mario; Karchin, Rachel; Mooney, Sean D

    2017-09-01

    The advent of next-generation sequencing has dramatically decreased the cost for whole-genome sequencing and increased the viability for its application in research and clinical care. The Personal Genome Project (PGP) provides unrestricted access to genomes of individuals and their associated phenotypes. This resource enabled the Critical Assessment of Genome Interpretation (CAGI) to create a community challenge to assess the bioinformatics community's ability to predict traits from whole genomes. In the CAGI PGP challenge, researchers were asked to predict whether an individual had a particular trait or profile based on their whole genome. Several approaches were used to assess submissions, including ROC AUC (area under receiver operating characteristic curve), probability rankings, the number of correct predictions, and statistical significance simulations. Overall, we found that prediction of individual traits is difficult, relying on a strong knowledge of trait frequency within the general population, whereas matching genomes to trait profiles relies heavily upon a small number of common traits including ancestry, blood type, and eye color. When a rare genetic disorder is present, profiles can be matched when one or more pathogenic variants are identified. Prediction accuracy has improved substantially over the last 6 years due to improved methodology and a better understanding of features. © 2017 Wiley Periodicals, Inc.

  14. Understanding the Human Genome Project — A Fact Sheet | NIH MedlinePlus the Magazine

    Science.gov (United States)

    ... The Human Genome Project spurred a revolution in biotechnology innovation around the world and played a key ... the U.S. the global leader in the new biotechnology sector. In April 2003, researchers successfully completed the ...

  15. National human genome projects: an update and an agenda

    OpenAIRE

    An, Joon Yong

    2017-01-01

    Population genetic and human genetic studies are being accelerated with genome technology and data sharing. Accordingly, in the past 10 years, several countries have initiated genetic research using genome technology and identified the genetic architecture of the ethnic groups living in the corresponding country or suggested the genetic foundation of a social phenomenon. Genetic research has been conducted from epidemiological studies that previously described the health or disease conditions...

  16. Massachusetts Crystalline Repository Project. Progress report, December 31, 1985

    International Nuclear Information System (INIS)

    1985-01-01

    Project activities which have been undertaken include the following: review and comment on OCRD projects reports; review of pertinent DOE, NRC, EPA, and DOT quidelines and regulations; review of reports and maps released by the federal project group and contractors; attendance at DOE workshops and conferences; implementation of state-specific research activities; interaction with representatives of federal agencies and other participating states; and interface with media, state officials and legislators, and interested citizens

  17. Projects at the component development and integration facility. Quarterly technical progress report, April 1, 1994--June 30, 1994

    International Nuclear Information System (INIS)

    1994-01-01

    This quarterly technical progress report presents progress on the projects at the Component Development and Integration Facility (CDIF) during the third quarter of FY94. The CDIF is a major Department of Energy test facility in Butte, Montana, operated by MSE, Inc. Projects in progress include: Biomass Remediation Project; Heavy Metal-Contaminated Soil Project; MHD Shutdown; Mine Waste Technology Pilot Program; Plasma Projects; Resource Recovery Project; and Spray Casting Project

  18. The Human Genome Project: Information access, management, and regulation. Final report

    Energy Technology Data Exchange (ETDEWEB)

    McInerney, J.D.; Micikas, L.B.

    1996-08-31

    The Human Genome Project is a large, internationally coordinated effort in biological research directed at creating a detailed map of human DNA. This report describes the access of information, management, and regulation of the project. The project led to the development of an instructional module titled The Human Genome Project: Biology, Computers, and Privacy, designed for use in high school biology classes. The module consists of print materials and both Macintosh and Windows versions of related computer software-Appendix A contains a copy of the print materials and discs containing the two versions of the software.

  19. A locally funded Puerto Rican parrot (Amazona vittata genome sequencing project increases avian data and advances young researcher education

    Directory of Open Access Journals (Sweden)

    Oleksyk Taras K

    2012-09-01

    Full Text Available Abstract Background Amazona vittata is a critically endangered Puerto Rican endemic bird, the only surviving native parrot species in the United States territory, and the first parrot in the large Neotropical genus Amazona, to be studied on a genomic scale. Findings In a unique community-based funded project, DNA from an A. vittata female was sequenced using a HiSeq Illumina platform, resulting in a total of ~42.5 billion nucleotide bases. This provided approximately 26.89x average coverage depth at the completion of this funding phase. Filtering followed by assembly resulted in 259,423 contigs (N50 = 6,983 bp, longest = 75,003 bp, which was further scaffolded into 148,255 fragments (N50 = 19,470, longest = 206,462 bp. This provided ~76% coverage of the genome based on an estimated size of 1.58 Gb. The assembled scaffolds allowed basic genomic annotation and comparative analyses with other available avian whole-genome sequences. Conclusions The current data represents the first genomic information from and work carried out with a unique source of funding. This analysis further provides a means for directed training of young researchers in genetic and bioinformatics analyses and will facilitate progress towards a full assembly and annotation of the Puerto Rican parrot genome. It also adds extensive genomic data to a new branch of the avian tree, making it useful for comparative analyses with other avian species. Ultimately, the knowledge acquired from these data will contribute to an improved understanding of the overall population health of this species and aid in ongoing and future conservation efforts.

  20. The Human Genome Project: applications in the diagnosis and treatment of neurologic disease.

    Science.gov (United States)

    Evans, G A

    1998-10-01

    The Human Genome Project (HGP), an international program to decode the entire DNA sequence of the human genome in 15 years, represents the largest biological experiment ever conducted. This set of information will contain the blueprint for the construction and operation of a human being. While the primary driving force behind the genome project is the potential to vastly expand the amount of genetic information available for biomedical research, the ramifications for other fields of study in biological research, the biotechnology and pharmaceutical industry, our understanding of evolution, effects on agriculture, and implications for bioethics are likely to be profound.

  1. PBX1 Genomic Pioneer Function Drives ERα Signaling Underlying Progression in Breast Cancer

    Science.gov (United States)

    Magnani, Luca; Ballantyne, Elizabeth B.; Zhang, Xiaoyang; Lupien, Mathieu

    2011-01-01

    Altered transcriptional programs are a hallmark of diseases, yet how these are established is still ill-defined. PBX1 is a TALE homeodomain protein involved in the development of different types of cancers. The estrogen receptor alpha (ERα) is central to the development of two-thirds of all breast cancers. Here we demonstrate that PBX1 acts as a pioneer factor and is essential for the ERα-mediated transcriptional response driving aggressive tumors in breast cancer. Indeed, PBX1 expression correlates with ERα in primary breast tumors, and breast cancer cells depleted of PBX1 no longer proliferate following estrogen stimulation. Profiling PBX1 recruitment and chromatin accessibility across the genome of breast cancer cells through ChIP-seq and FAIRE-seq reveals that PBX1 is loaded and promotes chromatin openness at specific genomic locations through its capacity to read specific epigenetic signatures. Accordingly, PBX1 guides ERα recruitment to a specific subset of sites. Expression profiling studies demonstrate that PBX1 controls over 70% of the estrogen response. More importantly, the PBX1-dependent transcriptional program is associated with poor-outcome in breast cancer patients. Correspondingly, PBX1 expression alone can discriminate a priori the outcome in ERα-positive breast cancer patients. These features are markedly different from the previously characterized ERα-associated pioneer factor FoxA1. Indeed, PBX1 is the only pioneer factor identified to date that discriminates outcome such as metastasis in ERα-positive breast cancer patients. Together our results reveal that PBX1 is a novel pioneer factor defining aggressive ERα-positive breast tumors, as it guides ERα genomic activity to unique genomic regions promoting a transcriptional program favorable to breast cancer progression. PMID:22125492

  2. Microenvironmental Heterogeneity Parallels Breast Cancer Progression: A Histology-Genomic Integration Analysis.

    Directory of Open Access Journals (Sweden)

    Rachael Natrajan

    2016-02-01

    Full Text Available The intra-tumor diversity of cancer cells is under intense investigation; however, little is known about the heterogeneity of the tumor microenvironment that is key to cancer progression and evolution. We aimed to assess the degree of microenvironmental heterogeneity in breast cancer and correlate this with genomic and clinical parameters.We developed a quantitative measure of microenvironmental heterogeneity along three spatial dimensions (3-D in solid tumors, termed the tumor ecosystem diversity index (EDI, using fully automated histology image analysis coupled with statistical measures commonly used in ecology. This measure was compared with disease-specific survival, key mutations, genome-wide copy number, and expression profiling data in a retrospective study of 510 breast cancer patients as a test set and 516 breast cancer patients as an independent validation set. In high-grade (grade 3 breast cancers, we uncovered a striking link between high microenvironmental heterogeneity measured by EDI and a poor prognosis that cannot be explained by tumor size, genomics, or any other data types. However, this association was not observed in low-grade (grade 1 and 2 breast cancers. The prognostic value of EDI was superior to known prognostic factors and was enhanced with the addition of TP53 mutation status (multivariate analysis test set, p = 9 × 10-4, hazard ratio = 1.47, 95% CI 1.17-1.84; validation set, p = 0.0011, hazard ratio = 1.78, 95% CI 1.26-2.52. Integration with genome-wide profiling data identified losses of specific genes on 4p14 and 5q13 that were enriched in grade 3 tumors with high microenvironmental diversity that also substratified patients into poor prognostic groups. Limitations of this study include the number of cell types included in the model, that EDI has prognostic value only in grade 3 tumors, and that our spatial heterogeneity measure was dependent on spatial scale and tumor size.To our knowledge, this is the first

  3. [Shippingport Station Decommissioning Project]: FY 1987 annual progress report, October 1, 1986-September 30, 1987

    International Nuclear Information System (INIS)

    1988-01-01

    This report presents progress on the Shippingport Station Decommissioning Project for FY 1987. There are two main topics: Project Management and Decommissioning Project Activities. Changes from technical and managerial concepts developed in the original Decommissioning Plan are presented with the related technical, economic, or schedule considerations. 3 refs., 9 figs., 4 tabs

  4. UK Safeguards R and D Project progress report for the period July 1983 - April 1984

    International Nuclear Information System (INIS)

    Adams, J.M.

    1984-10-01

    Progress reports are presented on the following projects: centrifuge enrichment plant safeguards; stores safeguards and general accounting techniques; generic programmes (projects underlying many instrument systems (e.g. tamper proofing and indication; neutron interrogation systems); system studies); FBR fuel cycle safeguards; service programmes (services to the IAEA); exploratory and short projects. (U.K.)

  5. Shippingport Station Decommissioning Project: FY 1986 annual progress report, October 1, 1985 through September 30, 1986

    International Nuclear Information System (INIS)

    1987-01-01

    This report presents progress on the Shippingport Station Decommissioning Project for FY 1986. There are two main topics: Project Management and Decommissioning Project Activities. Changes from technical and managerial concepts developed in the original Decommissioning Plan are presented with the related technical, economic, or schedule considerations. 9 refs., 4 figs., 3 tabs

  6. Shippingport Station Decommissioning Project: FY 1988 annual progress report, October 1, 1987--September 30, 1988

    International Nuclear Information System (INIS)

    1989-01-01

    This report presents progress on the Shippingport Station Decommissioning Project for FY 1988. There are two main topics: Project Management and Decommissioning Project Activities. Changes from technical and managerial concepts developed in the original Decommissioning Plan are presented with the related technical, economic, or schedular considerations. 4 refs., 17 figs., 2 tabs

  7. SEAFOODplus: objectives, outputs, progress since the completion of the project

    DEFF Research Database (Denmark)

    Børresen, Torger

    the human nutrition area one project pointed toward new information indicating that fish protein has been overlooked as a key factor for human nutrition. When male individuals received a hypocaloric diet for weight reduction, the weight loss was significantly higher when the diet contained fish...... for delivering high amounts of essential nutrients, e.g. selenium, through optimal feed formulations, thus using fish as a carrier of important nutrients in the human diet. Traceability was a project area cutting across all the other areas within the SEAFOODplus project. New methods for implementing full...

  8. Progress Report of the Schools Television Research Project--III

    Science.gov (United States)

    Kemelfield, Graeme

    1969-01-01

    "This concluding article provides the first published account of a series of psychological experiments which are presently being undertaken by the Schools Television Research Project, examining presentation factors in instructional television. (Editor)

  9. Progress on the HUPO Draft Human Proteome: 2017 Metrics of the Human Proteome Project.

    Science.gov (United States)

    Omenn, Gilbert S; Lane, Lydie; Lundberg, Emma K; Overall, Christopher M; Deutsch, Eric W

    2017-12-01

    The Human Proteome Organization (HUPO) Human Proteome Project (HPP) continues to make progress on its two overall goals: (1) completing the protein parts list, with an annual update of the HUPO draft human proteome, and (2) making proteomics an integrated complement to genomics and transcriptomics throughout biomedical and life sciences research. neXtProt version 2017-01-23 has 17 008 confident protein identifications (Protein Existence [PE] level 1) that are compliant with the HPP Guidelines v2.1 ( https://hupo.org/Guidelines ), up from 13 664 in 2012-12 and 16 518 in 2016-04. Remaining to be found by mass spectrometry and other methods are 2579 "missing proteins" (PE2+3+4), down from 2949 in 2016. PeptideAtlas 2017-01 has 15 173 canonical proteins, accounting for nearly all of the 15 290 PE1 proteins based on MS data. These resources have extensive data on PTMs, single amino acid variants, and splice isoforms. The Human Protein Atlas v16 has 10 492 highly curated protein entries with tissue and subcellular spatial localization of proteins and transcript expression. Organ-specific popular protein lists have been generated for broad use in quantitative targeted proteomics using SRM-MS or DIA-SWATH-MS studies of biology and disease.

  10. Construction progress of the cooling & ventilation in the LHC project

    CERN Document Server

    Body, Y; Josa, F; Monsted, A; Pirollet, B; CERN. Geneva. ST Division

    2002-01-01

    After the LEP dismantling Phase the Cooling and Ventilation Group has started the LHC construction work. Year 2001 through to 2004 will certainly be the most important period of activity for the CV group in the erection phase The author will report on the current works that are in progress on the different LHC Points distinguishing between the Ventilation and the Water Cooling installations. The Ventilation work completed in the new surface buildings in Points 1, 4,5,6 and 8. The work for the Cooling plants comprehend to the pumping stations, the cooling towers and the chilled water production stations in Points 1 and 5, For all of these activities, an updated report of the progress the work, the planning and of the expenses are given. Finally, a brief overview of the future activities is presented.

  11. Progress, status, and plans for the HRIBF project

    International Nuclear Information System (INIS)

    Auble, R.L.; Alton, G.D.; Bailey, J.D.

    1995-01-01

    Over the last three years, the Holifield accelerator system has been reconfigured into a first-generation radioactive ion beam facility, the HRIBF, a national user facility for RIB research. The construction and reconfiguration have been completed and the equipment commissioning and beam development phases have started. The progress to date, the present status, and future plans will be given. The special problems connected with the production and acceleration of RIBs will be discussed

  12. Jules Horowitz reactor - presentation of the project and progress report

    International Nuclear Information System (INIS)

    Ballagny, A.; Frachet, S.; Rommens, M.; Guigon, B.; Minguet, J.L.; Dupuy, J.P.; Leydier, C.

    2001-01-01

    The RJH project was launched by CEA some years ago, with the objective to replace, after 2010, the material testing reactors of the previous generation. The objectives are also: to realise a significant step in term of performances, to ensure a high flexibility of the design, in order to host in the future new experiments, which are not completely defined at the project stage, to reach a high level of safety, according to the best current practice. After a summary of the main experimental objectives of the facility, the present paper deals with a detailed technical presentation of the project, resulting from preliminary design studies. The following topics are covered successively: the main functionalities, the resulting design options and technical solutions, the layout of the nuclear facility. (orig.)

  13. Progress and achievements of the ITER L-4 blanket project

    International Nuclear Information System (INIS)

    Daenner, W.; Toschi, R.; Cardella, A.

    1999-01-01

    The L-4 Blanket Project embraces the R and D of the ITER Shielding Blanket, and its main objective is the fabrication of prototype components. This paper summarises the main conclusions from the materials R and D and the development of technologies which were required for the prototype specifications and manufacturing. The main results of the ongoing testing activities, and of the component manufacture are outlined.The main objectives of the project have been achieved including improvements of the material properties and of joining technologies, which resulted in good component quality and high performance in qualification tests. (author)

  14. Progress and achievements of the ITER L-4 blanket project

    International Nuclear Information System (INIS)

    Daenner, W.; Toschi, R.; Cardella, A.

    2001-01-01

    The L-4 Blanket Project embraces the R and D of the ITER Shielding Blanket, and its main objective is the fabrication of prototype components. This paper summarises the main conclusions from the materials R and D and the development of technologies which were required for the prototype specifications and manufacturing. The main results of the ongoing testing activities, and of the component manufacture are outlined. The main objectives of the project have been achieved including improvements of the material properties and of joining technologies, which resulted in good component quality and high performance in qualification tests. (author)

  15. 7 CFR 3405.19 - Monitoring progress of funded projects.

    Science.gov (United States)

    2010-01-01

    ... RESEARCH, EDUCATION, AND EXTENSION SERVICE, DEPARTMENT OF AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS... reform, and opportunities to enhance dissemination of exemplary end products/results. (b) An Annual..., including: A review of project objectives and accomplishments; a description of any products and outcomes...

  16. Savanna ecosystem project: phase I summary and phase II progress

    CSIR Research Space (South Africa)

    Huntely, BJ

    1978-07-01

    Full Text Available A summary of the results of the first phase (mid 1974 to mid 1976) of the South African Savanna Ecosystem Project being undertaken at Nylsvley in the northern Transvaal is presented. Phase I of this ten year study of the structure and functioning...

  17. 7 CFR 3402.23 - Documentation of progress on funded projects.

    Science.gov (United States)

    2010-01-01

    ... RESEARCH, EDUCATION, AND EXTENSION SERVICE, DEPARTMENT OF AGRICULTURE FOOD AND AGRICULTURAL SCIENCES... of Fellows supported by any special international study or thesis/dissertation research allowance and... Research Information System (CRIS). The CRIS database contains narrative project information, progress...

  18. Ceramic Technology Project. Semiannual progress report, April 1991--September 1991

    Energy Technology Data Exchange (ETDEWEB)

    1992-03-01

    The Ceramic Technology Project was developed by the USDOE Office of Transportation Systems (OTS) in Conservation and Renewable Energy. This project, part of the OTS`s Materials Development Program, was developed to meet the ceramic technology requirements of the OTS`s automotive technology programs. Significant accomplishments in fabricating ceramic components for the USDOE and NASA advanced heat engine programs have provided evidence that the operation of ceramic parts in high-temperature engine environments is feasible. These programs have also demonstrated that additional research is needed in materials and processing development, design methodology, and data base and life prediction before industry will have a sufficient technology base from which to produce reliable cost-effective ceramic engine components commercially. A five-year project plan was developed with extensive input from private industry. In July 1990 the original plan was updated through the estimated completion of development in 1993. The objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. The project approach includes determining the mechanisms controlling reliability, improving processes for fabricating existing ceramics, developing new materials with increased reliability, and testing these materials in simulated engine environments to confirm reliability. Although this is a generic materials project, the focus is on the structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines. To facilitate the rapid transfer of this technology to US industry, the major portion of the work is being done in the ceramic industry, with technological support from government laboratories, other industrial laboratories, and universities.

  19. Progress in resolving construction issues on nuclear projects

    International Nuclear Information System (INIS)

    Reedy, R.F.

    1985-01-01

    The cost of nuclear plant construction in the United States can be drastically reduced. Progress is being made in the reduction of construction costs and with continuing effort much more can be done. The easiest way to attack high costs is to eliminate the unnecessary repairs and rework in construction, along with the time consuming evaluations and dispositions, and to use common sense in design practices. By doing this, millions of dollars could easily be cut from the cost of construction of nuclear powerplants

  20. Athlome Project Consortium: a concerted effort to discover genomic and other "omic" markers of athletic performance.

    Science.gov (United States)

    Pitsiladis, Yannis P; Tanaka, Masashi; Eynon, Nir; Bouchard, Claude; North, Kathryn N; Williams, Alun G; Collins, Malcolm; Moran, Colin N; Britton, Steven L; Fuku, Noriyuki; Ashley, Euan A; Klissouras, Vassilis; Lucia, Alejandro; Ahmetov, Ildus I; de Geus, Eco; Alsayrafi, Mohammed

    2016-03-01

    Despite numerous attempts to discover genetic variants associated with elite athletic performance, injury predisposition, and elite/world-class athletic status, there has been limited progress to date. Past reliance on candidate gene studies predominantly focusing on genotyping a limited number of single nucleotide polymorphisms or the insertion/deletion variants in small, often heterogeneous cohorts (i.e., made up of athletes of quite different sport specialties) have not generated the kind of results that could offer solid opportunities to bridge the gap between basic research in exercise sciences and deliverables in biomedicine. A retrospective view of genetic association studies with complex disease traits indicates that transition to hypothesis-free genome-wide approaches will be more fruitful. In studies of complex disease, it is well recognized that the magnitude of genetic association is often smaller than initially anticipated, and, as such, large sample sizes are required to identify the gene effects robustly. A symposium was held in Athens and on the Greek island of Santorini from 14-17 May 2015 to review the main findings in exercise genetics and genomics and to explore promising trends and possibilities. The symposium also offered a forum for the development of a position stand (the Santorini Declaration). Among the participants, many were involved in ongoing collaborative studies (e.g., ELITE, GAMES, Gene SMART, GENESIS, and POWERGENE). A consensus emerged among participants that it would be advantageous to bring together all current studies and those recently launched into one new large collaborative initiative, which was subsequently named the Athlome Project Consortium. Copyright © 2016 the American Physiological Society.

  1. Application of genomics-assisted breeding for generation of climate resilient crops: Progress and prospects

    Directory of Open Access Journals (Sweden)

    Chittaranjan eKole

    2015-08-01

    Full Text Available Climate change affects agricultural productivity worldwide. Increased prices of food commodities are the initial indication of drastic edible yield loss, which is expected to surge further due to global warming. This situation has compelled plant scientists to develop climate change-resilient crops, which can withstand broad-spectrum stresses such as drought, heat, cold, salinity, flood and submergence, and pests along with increased productivity. Genomics appears to be a promising tool for deciphering the stress responsiveness of crop species with adaptation traits or in wild relatives towards identifying underlying genes, alleles or quantitative trait loci. Molecular breeding approaches have been proven helpful in enhancing the stress adaptation of crop plants, and recent advancement in next-generation sequencing along with high-throughput sequencing and phenotyping platforms have transformed molecular breeding to genomics-assisted breeding (GAB. In view of this, the present review elaborates the progress and prospects of GAB in improving climate change resilience in crop plants towards circumventing global food insecurity.

  2. The Recon Pilot Project: A Progress Report, October 1970-May 1971

    Directory of Open Access Journals (Sweden)

    Henriette D. Avram

    1971-09-01

    Full Text Available Synopsis of three progress reports on the RECON Pilot Project submitted by the Library of Congress to the Council on Library Resources covering the period October 1970-May 1971. Progress is reported in the following areas: RECON production, foreign language editing test, format recognition, microfilming, input devices, and tasks assigned to the RECON Working Task Force.

  3. Aircraft Nuclear Propulsion Project Quarterly Progress Report for Period Ending December 31, 1956

    Energy Technology Data Exchange (ETDEWEB)

    NA, NA [ORNL

    1957-03-12

    This quarterly progress report of the Aircraft Nuclear Propulsion Project at ORNL records the technical progress of research on circulating-fuel reactors and other ANP research at the Laboratory. The report is divided into five major parts: 1) Aircraft Reactor Engineering, 2) Chemistry, and 3) Metallurgy, 4) Heat Transfer and Physical Properties, Radiation Damage, and Fuel Recovery and Reprocessing, and 5) Reactor Shielding.

  4. Human genome education model project. Ethical, legal, and social implications of the human genome project: Education of interdisciplinary professionals

    Energy Technology Data Exchange (ETDEWEB)

    Weiss, J.O. [Alliance of Genetic Support Groups, Chevy Chase, MD (United States); Lapham, E.V. [Georgetown Univ., Washington, DC (United States). Child Development Center

    1996-12-31

    This meeting was held June 10, 1996 at Georgetown University. The purpose of this meeting was to provide a multidisciplinary forum for exchange of state-of-the-art information on the human genome education model. Topics of discussion include the following: psychosocial issues; ethical issues for professionals; legislative issues and update; and education issues.

  5. Staunton 1 Reclamation Demonstration Project. Progress report for 1977

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-12-01

    The Staunton 1 Reclamation Demonstration Project involves an evaluation of the reclamation process for a deep coal mine refuse system. A typical abandoned midwestern deep coal mine refuse site was selected, final land use was determined, baseline data were collected, engineering plans were developed and implemented, and a post-construction evaluation was begun. The project is a cooperative effort by two state agencies--the Abandoned Mined Land Reclamation Council of Illinois the Illinois Institute for Environmental Quality--and the U.S. Department of Energy through the Land Reclamation Program at Argonne National Laboratory. Current investigations are monitoring groundwater, surface water quality, aquatic ecosystems, revegetation, soil characteristics, erosion and runoff, soil microbial and soil fauna populations, wildlife, and economic effects of the reclamation effort. The research is a multidisciplinary approach to the concept of ecosystem response to reclamation.

  6. Milliwatt Generator Project. Progress report, April-September 1980

    International Nuclear Information System (INIS)

    Maraman, W.J.

    1981-01-01

    This formal biannual report covers the effort related to the Milliwatt Generator Project (MWG) carried out for the Department of Energy, Office of Military Applications, by the Los Alamos Scientific Laboratory (LASL). Most of the studies discussed here are of a continuing nature. Results and conclusions may change as the work continues. Published reference to the results cited in this report should not be made without the explicit permission of the person in charge of the work

  7. Oman- Decisive progress for the Liquefied Natural Gas (LNG) project

    International Nuclear Information System (INIS)

    Anon.

    1996-01-01

    The soon signature of a firm contract with the Korea Gas Corporation and the perspective of an agreement with Thailand will allow the attribution of a construction contract in September 1996. The hypothesis of a third liquefaction train seems to be more and more probable. The project of Oman-India gas pipeline is not given up but only put it back to several years. (N.C.)

  8. Advanced Neutron Source (ANS) Project Progress report, FY 1991

    International Nuclear Information System (INIS)

    Campbell, J.H.; Selby, D.L.; Harrington, R.M.; Thompson, P.B.

    1992-01-01

    This report discusses the following about the Advanced Neutron Source: Project Management; Research and Development; Fuel Development; Corrosion Loop Tests and Analyses; Thermal-Hydraulic Loop Tests; Reactor Control and Shutdown Concepts; Critical and Subcritical Experiments; Material Data, Structural Tests, and Analysis; Cold-Source Development; Beam Tube, Guide, and Instrument Development; Hot-Source Development; Neutron Transport and Shielding; I ampersand C Research and Development; Design; and Safety

  9. Research in progress: FY 1992. Summaries of projects

    Energy Technology Data Exchange (ETDEWEB)

    1993-08-01

    The Biological and Environmental Research (BER) Program of OHER has two main missions: (1) to develop the knowledge base necessary to identify, understand, and anticipate the long-term health and environmental consequences of energy use and development and (2) to utilize the Department`s unique scientific and technological capabilities to solve major scientific problems in medicine, biology, and the environment. These missions reflect a commitment to develop the beneficial uses of advanced energy technologies while at the same time assuring that any potentially adverse health and environmental impacts of the Nation`s energy policies are fully identified and understood. The BER Program includes research in atmospheric, marine, and terrestrial processes, including the linkage between the use in greenhouse gases, carbon dioxide, and regional and global climate change; in molecular and subcellular mechanisms underlying human somatic and genetic processes and their responses to energy-related environmental toxicants; in nuclear medicine, structural biology, the human genome, measurement sciences and instrumentation, and other areas that require the unique capabilities of the Department`s laboratory system. The principal areas of research are Health Research and Environmental Research.

  10. Clonal expansion and linear genome evolution through breast cancer progression from pre-invasive stages to asynchronous metastasis

    DEFF Research Database (Denmark)

    Krøigård, Anne Bruun; Larsen, Martin Jakob; Lænkholm, Anne Vibeke

    2015-01-01

    Evolution of the breast cancer genome from pre-invasive stages to asynchronous metastasis is complex and mostly unexplored, but highly demanded as it may provide novel markers for and mechanistic insights in cancer progression. The increasing use of personalized therapy of breast cancer necessita......Evolution of the breast cancer genome from pre-invasive stages to asynchronous metastasis is complex and mostly unexplored, but highly demanded as it may provide novel markers for and mechanistic insights in cancer progression. The increasing use of personalized therapy of breast cancer...... progression from one breast cancer patient, including two different regions of Ductal Carcinoma In Situ (DCIS), primary tumor and an asynchronous metastasis. We identify a remarkable landscape of somatic mutations, retained throughout breast cancer progression and with new mutational events emerging at each...

  11. Enabling a community to dissect an organism: overview of the Neurospora functional genomics project.

    Science.gov (United States)

    Dunlap, Jay C; Borkovich, Katherine A; Henn, Matthew R; Turner, Gloria E; Sachs, Matthew S; Glass, N Louise; McCluskey, Kevin; Plamann, Michael; Galagan, James E; Birren, Bruce W; Weiss, Richard L; Townsend, Jeffrey P; Loros, Jennifer J; Nelson, Mary Anne; Lambreghts, Randy; Colot, Hildur V; Park, Gyungsoon; Collopy, Patrick; Ringelberg, Carol; Crew, Christopher; Litvinkova, Liubov; DeCaprio, Dave; Hood, Heather M; Curilla, Susan; Shi, Mi; Crawford, Matthew; Koerhsen, Michael; Montgomery, Phil; Larson, Lisa; Pearson, Matthew; Kasuga, Takao; Tian, Chaoguang; Baştürkmen, Meray; Altamirano, Lorena; Xu, Junhuan

    2007-01-01

    A consortium of investigators is engaged in a functional genomics project centered on the filamentous fungus Neurospora, with an eye to opening up the functional genomic analysis of all the filamentous fungi. The overall goal of the four interdependent projects in this effort is to accomplish functional genomics, annotation, and expression analyses of Neurospora crassa, a filamentous fungus that is an established model for the assemblage of over 250,000 species of non yeast fungi. Building from the completely sequenced 43-Mb Neurospora genome, Project 1 is pursuing the systematic disruption of genes through targeted gene replacements, phenotypic analysis of mutant strains, and their distribution to the scientific community at large. Project 2, through a primary focus in Annotation and Bioinformatics, has developed a platform for electronically capturing community feedback and data about the existing annotation, while building and maintaining a database to capture and display information about phenotypes. Oligonucleotide-based microarrays created in Project 3 are being used to collect baseline expression data for the nearly 11,000 distinguishable transcripts in Neurospora under various conditions of growth and development, and eventually to begin to analyze the global effects of loss of novel genes in strains created by Project 1. cDNA libraries generated in Project 4 document the overall complexity of expressed sequences in Neurospora, including alternative splicing alternative promoters and antisense transcripts. In addition, these studies have driven the assembly of an SNP map presently populated by nearly 300 markers that will greatly accelerate the positional cloning of genes.

  12. Plutonium Reclamation Facility incident response project progress report

    International Nuclear Information System (INIS)

    Austin, B.A.

    1997-01-01

    This report provides status of Hanford activities in response to process deficiencies highlighted during and in response to the May 14, 1997, explosion at the Plutonium Reclamation Facility. This report provides specific response to the August 4, 1997, memorandum from the Secretary which requested a progress report, in 120 days, on activities associated with reassessing the known and evaluating new vulnerabilities (chemical and radiological) at facilities that have been shut down, are in standby, are being deactivated or have otherwise changed their conventional mode of operation in the last several years. In addition, this report is intended to provide status on emergency response corrective activities as requested in the memorandum from the Secretary on August 28, 1997. Status is also included for actions requested in the second August 28, 1997, memorandum from the Secretary, regarding timely notification of emergencies

  13. Plutonium Reclamation Facility incident response project progress report

    Energy Technology Data Exchange (ETDEWEB)

    Austin, B.A.

    1997-11-25

    This report provides status of Hanford activities in response to process deficiencies highlighted during and in response to the May 14, 1997, explosion at the Plutonium Reclamation Facility. This report provides specific response to the August 4, 1997, memorandum from the Secretary which requested a progress report, in 120 days, on activities associated with reassessing the known and evaluating new vulnerabilities (chemical and radiological) at facilities that have been shut down, are in standby, are being deactivated or have otherwise changed their conventional mode of operation in the last several years. In addition, this report is intended to provide status on emergency response corrective activities as requested in the memorandum from the Secretary on August 28, 1997. Status is also included for actions requested in the second August 28, 1997, memorandum from the Secretary, regarding timely notification of emergencies.

  14. Competence development organizations in project management on the basis of genomic model methodologies

    OpenAIRE

    Бушуев, Сергей Дмитриевич; Рогозина, Виктория Борисовна; Ярошенко, Юрий Федерович

    2013-01-01

    The matrix technology for identification of organisational competencies in project management is presented in the article. Matrix elements are the components of organizational competence in the field of project management and project management methodology represented in the structure of the genome. The matrix model of competence in the framework of the adopted methodologies and scanning method for identifying organizational competences formalised. Proposed methods for building effective proj...

  15. Genomics England's implementation of its public engagement strategy: Blurred boundaries between engagement for the United Kingdom's 100,000 Genomes project and the need for public support.

    Science.gov (United States)

    Samuel, Gabrielle Natalie; Farsides, Bobbie

    2018-04-01

    The United Kingdom's 100,000 Genomes Project has the aim of sequencing 100,000 genomes from National Health Service patients such that whole genome sequencing becomes routine clinical practice. It also has a research-focused goal to provide data for scientific discovery. Genomics England is the limited company established by the Department of Health to deliver the project. As an innovative scientific/clinical venture, it is interesting to consider how Genomics England positions itself in relation to public engagement activities. We set out to explore how individuals working at, or associated with, Genomics England enacted public engagement in practice. Our findings show that individuals offered a narrative in which public engagement performed more than one function. On one side, public engagement was seen as 'good practice'. On the other, public engagement was presented as core to the project's success - needed to encourage involvement and ultimately recruitment. We discuss the implications of this in this article.

  16. Advanced Neutron Source (ANS) Project progress report, FY 1994

    Energy Technology Data Exchange (ETDEWEB)

    Campbell, J.H.; King-Jones, K.H. [eds.; Selby, D.L.; Harrington, R.M. [Oak Ridge National Lab., TN (United States); Thompson, P.B. [Martin Marietta Energy Systems, Inc., Oak Ridge, TN (United States). Central Engineering Services

    1995-01-01

    The President`s budget request for FY 1994 included a construction project for the Advanced Neutron Source (ANS). However, the budget that emerged from the Congress did not, and so activities during this reporting period were limited to continued research and development and to advanced conceptual design. A significant effort was devoted to a study, requested by the US Department of Energy (DOE) and led by Brookhaven National Laboratory, of the performance and cost impacts of reducing the uranium fuel enrichment below the baseline design value of 93%. The study also considered alternative core designs that might mitigate those impacts. The ANS Project proposed a modified core design, with three fuel elements instead of two, that would allow operation with only 50% enriched uranium and use existing fuel technology. The performance penalty would be 15--20% loss of thermal neutron flux; the flux would still just meet the minimum design requirement set by the user community. At the time of this writing, DOE has not established an enrichment level for ANS, but two advisory committees have recommended adopting the new core design, provided the minimum flux requirements are still met.

  17. Tunneling on the Yucca Mountain Project: Progress and lessons learned

    International Nuclear Information System (INIS)

    Hansmire, W.H.; Rogers, D.J.; Wightman, W.D.

    1996-01-01

    The Yucca Mountain Site Characterization Project is the US's effort to confirm the technical acceptability of Yucca Mountain as a repository for high-level nuclear waste. A key part of the site characterization project is the construction of a 7.8-km-long, 7.6-m-diameter tunnel for in-depth geologic and other scientific investigations. The work is governed in varying degrees by the special requirements for nuclear quality assurance, which imposes uncommon and often stringent limitations on the materials which can be used in construction, the tunneling methods and procedures used, and record-keeping for many activities. This paper presents the current status of what has been learned, how construction has adapted to meet the requirements, and how the requirements were interpreted in a mitigating way to meet the legal obligations, yet build the tunnel as rapidly as possible. With regard to design methodologies and the realities of tunnel construction, ground support with a shielded Tunnel Boring Machine is discussed. Notable lessons learned include the need for broad design analyses for a wide variety of conditions and how construction procedures affect ground support

  18. Advanced Neutron Source (ANS) Project progress report, FY 1994

    International Nuclear Information System (INIS)

    Campbell, J.H.; King-Jones, K.H.; Thompson, P.B.

    1995-01-01

    The President's budget request for FY 1994 included a construction project for the Advanced Neutron Source (ANS). However, the budget that emerged from the Congress did not, and so activities during this reporting period were limited to continued research and development and to advanced conceptual design. A significant effort was devoted to a study, requested by the US Department of Energy (DOE) and led by Brookhaven National Laboratory, of the performance and cost impacts of reducing the uranium fuel enrichment below the baseline design value of 93%. The study also considered alternative core designs that might mitigate those impacts. The ANS Project proposed a modified core design, with three fuel elements instead of two, that would allow operation with only 50% enriched uranium and use existing fuel technology. The performance penalty would be 15--20% loss of thermal neutron flux; the flux would still just meet the minimum design requirement set by the user community. At the time of this writing, DOE has not established an enrichment level for ANS, but two advisory committees have recommended adopting the new core design, provided the minimum flux requirements are still met

  19. Progress of the LASL dry hot rock geothermal energy project

    Science.gov (United States)

    Smith, M. C.

    1974-01-01

    The possibilities and problems of extracting energy from geothermal reservoirs which do not spontaneously yield useful amounts of steam or hot water are discussed. The system for accomplishing this which is being developed first is a pressurized-water circulation loop intended for use in relatively impermeable hot rock. It will consist of two holes connected through the hot rock by a very large hydraulic fracture and connected at the surface through the primary heat exchanger of an energy utilization system. Preliminary experiments in a hole 2576 ft (0.7852 km) deep, extending about 470 ft (143 m) into the Precambrian basement rock underlying the Jemez Plateau of north-central New Mexico, revealed no unexpected difficulties in drilling or hydraulically fracturing such rock at a temperature of approximately 100 C, and demonstrated a permeability low enough so that it appeared probable that pressurized water could be contained by the basement rock. Similar experiments are in progress in a second hole, now 6701 ft (2.043 km) deep, about 1.5 miles (2.4 km) south of the first one.

  20. Progress of the intense positron beam project EPOS

    International Nuclear Information System (INIS)

    Krause-Rehberg, R.; Brauer, G.; Jungmann, M.; Krille, A.; Rogov, A.; Noack, K.

    2008-01-01

    EPOS (the ELBE POsitron Source) is a running project to build an intense, bunched positron beam for materials research. It makes use of the bunched electron beam of the ELBE radiation source (Electron Linac with high Brilliance and low Emittance) at the Research Centre Dresden-Rossendorf (40 MeV, 1 mA). ELBE has unique timing properties, the bunch length is <5 ps and the repetition time is 77 ns. In contrast to other Linacs made for Free Electron Lasers (e.g., TTF at DESY, Hamburg), ELBE can be operated in full cw-mode, i.e., with an uninterrupted sequence of bunches. The article continues an earlier publication. It concentrates on details of the timing system and describes issues of radiation protection

  1. NEPA Project quarterly progress report, April 1--June 30, 1950

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1950-12-31

    Exploration of various types of power plant cycles for nuclear propelled aircraft has been continued during this quarter. The principal current objective of the project is the development of information which will make an intelligent choice of the basic power plant cycle possible. It is still hoped that this choice can be made late in 1950. The survey studies which have been under way for several months continued during the quarter. These consist of analyses and rough preliminary layouts for various types of aircraft, using each of the several basic cycles which have been seriously considered for each of the three phases of development. Although it is still extremely premature to discuss the relative merits of the various cycles, the information so for developed discloses some cycle differences which may, if confirmed by additional work, be significant. In this respect, there have been no recent major changes in the comparative standings of the cycles.

  2. Genome-wide analysis of wild-type Epstein-Barr virus genomes derived from healthy individuals of the 1,000 Genomes Project.

    Science.gov (United States)

    Santpere, Gabriel; Darre, Fleur; Blanco, Soledad; Alcami, Antonio; Villoslada, Pablo; Mar Albà, M; Navarro, Arcadi

    2014-04-01

    Most people in the world (∼90%) are infected by the Epstein-Barr virus (EBV), which establishes itself permanently in B cells. Infection by EBV is related to a number of diseases including infectious mononucleosis, multiple sclerosis, and different types of cancer. So far, only seven complete EBV strains have been described, all of them coming from donors presenting EBV-related diseases. To perform a detailed comparative genomic analysis of EBV including, for the first time, EBV strains derived from healthy individuals, we reconstructed EBV sequences infecting lymphoblastoid cell lines (LCLs) from the 1000 Genomes Project. As strain B95-8 was used to transform B cells to obtain LCLs, it is always present, but a specific deletion in its genome sets it apart from natural EBV strains. After studying hundreds of individuals, we determined the presence of natural EBV in at least 10 of them and obtained a set of variants specific to wild-type EBV. By mapping the natural EBV reads into the EBV reference genome (NC007605), we constructed nearly complete wild-type viral genomes from three individuals. Adding them to the five disease-derived EBV genomic sequences available in the literature, we performed an in-depth comparative genomic analysis. We found that latency genes harbor more nucleotide diversity than lytic genes and that six out of nine latency-related genes, as well as other genes involved in viral attachment and entry into host cells, packaging, and the capsid, present the molecular signature of accelerated protein evolution rates, suggesting rapid host-parasite coevolution.

  3. Draft Genome Sequences of Two Propionibacterium acnes Strains Isolated from Progressive Macular Hypomelanosis Lesions of Human Skin

    DEFF Research Database (Denmark)

    Petersen, Rolf; Lomholt, Hans B.; Scholz, Christian F. P.

    2015-01-01

    Propionibacterium acnes is a Gram-positive bacterium that is prevalent on human skin. It has been associated with skin disorders such as acne vulgaris and progressive macular hypomelanosis (PMH). Here, we report draft genome sequences of two type III P. acnes strains, PMH5 and PMH7, isolated from...

  4. The RadGenomics project. Prediction for radio-susceptibility of individuals with genetic predisposition

    International Nuclear Information System (INIS)

    Imai, Takashi

    2003-01-01

    The ultimate goal of our project, named RadGenomics, is to elucidate the heterogeneity of the response to ionizing radiation arising from genetic variation among individuals, for the purpose of developing personalized radiation therapy regimens for cancer patients. Cancer patients exhibit patient-to-patient variability in normal tissue reactions after radiotherapy. Several observations support the hypothesis that the radiosensitivity of normal tissue is influenced by genetic factors. The rapid progression of human genome sequencing and the recent development of new technologies in molecular biology are providing new opportunities for elucidating the genetic basis of individual differences in susceptibility to radiation exposure. The development of a sufficiently robust, predictive assay enabling individual dose adjustment would improve the outcome of radiation therapy in patients. Our strategy for identification of DNA polymorphisms that contribute to the individual radiosensitivity is as follows. First, we have been categorizing DNA samples obtained from cancer patients, who have been kindly introduced to us through many collaborators, according to their clinical characteristics including the method and effect of treatment and side effects as scored by toxicity criteria, and also the result of an in vitro radiosensitivity assay, e.g., the micronuclei assay of their lymphocytes. Second, we have identified candidate genes for genotyping mainly by using our custom-designed oligonucleotide array with RNA samples, in which the probes were obtained from more than 40 cancer and 3 fibroblast cell lines whose radiosensitivity level was quite heterogeneous. We have also been studying the modification of proteins after irradiation of cells which may be caused by mainly phosphorylation or dephosphorylation, using mass spectrometry. Genes encoding the modified proteins and/or other proteins with which they interact such as specific protein kinases and phosphatases are also

  5. Progress Report 15, December 1979-April 1980, and proceedings of the fifteenth Project Integration Meeting

    Energy Technology Data Exchange (ETDEWEB)

    1980-01-01

    Progress made by the Low-Cost Solar Array Project during the period December 1979 to April 1980 is reported. Reports on project analysis and integration; technology development in silicon material, large-area silicon sheet and encapsulation; production process and equipment development; engineering; and operations are included. Also, a report on, and copies of visual presentations made at, the Project Integration Meeting held April 2 and 3, 1980, are included.

  6. The Human Genome Project and the social contract: a law policy approach.

    Science.gov (United States)

    Byk, C

    1992-08-01

    For the first time in history, genetics will enable science to completely identify each human as genetically unique. Will this knowledge reinforce the trend for more individual liberties or will it create a 'brave new world'? A law policy approach to the problems raised by the human genome project shows how far our democratic institutions are from being the proper forum to discuss such issues. Because of the fears and anxiety raised in the population, and also because of its wide implications on the everyday life, the human genome analysis more than any other project needs to succeed in setting up such a social assessment.

  7. Staunton 1 Reclamation Demonstration Project. Progress report II

    Energy Technology Data Exchange (ETDEWEB)

    None

    1979-07-01

    The Staunton 1 Reclamation Demonstration Project involves an evaluation of the reclamation process on a 13.8-ha abandoned deep coal mine refuse site in southwestern Illinois. The procedure included collection of preconstruction environmental data, determination of the site's final land use, and development and implementation of a detailed site development plan. Approximately 9.3 ha of refuse material was recontoured, covered with a minimum of 30 cm of soil obtained on site, and seeded with a mixture of grasses and legumes. Hydrologic investigation indicates some improvement in groundwater quality. Surface water quality also has shown improvement, but development of the aquatic ecosystem in the newly-constructed pond is slow. Revegetation has been successful, and a protective plant cover has been established on most areas of the site. Soil tests indicate that acceptable plant growth media have been constructed; however, continued application of fertilizer and limestone will probably be necessary to maintain the vegetation. The soil microbial community has achieved total numbers equal to those of old fields, but species' diversity is low. Small mammals, birds, reptiles, and amphibians have invaded and are utilizing the site. The economic value of the site and adjacent property has increased substantially, and the area's aesthetic value has been enhanced significantly. The two-year period of intensive monitoring and evaluation has been utilized to develop recommendations for improving the designs of future reclamation efforts.

  8. Progress of IFERC project in the Broader Approach Activities

    International Nuclear Information System (INIS)

    Araki, M.; Sakamoto, Y.; Hayashi, K.; Ohuchi, R.; Nishitani, T.

    2010-01-01

    For contributing to the ITER project and promoting a possible early realization of DEMO, since 2007 the International Fusion Energy Research Centre (IFERC) shall perform the activities on (1) DEMO Design and R and D Coordination, (2) Computational Simulation Centre, and (3) ITER Remote Experimentation Centre in the framework under the agreement between the Government of Japan and the European Atomic Energy Community for the joint implementation of the Broader Approach Activities in the field of fusion energy. The DEMO Design activity aims at establishing a common basis for DEMO Design, including design features of DEMO, possible common concepts of DEMO, a roadmap for DEMO, and so on. Based on the common interest of EU and Japan toward DEMO, the DEMO R and D activities have been planned and carried out for the areas which are related to DEMO blanket development: SiC/SiC composites, tritium technology, materials engineering, advanced neutron multiplier, and advanced tritium breeders for DEMO blanket. In the activity of the Computational Simulation Centre, the objective is to provide and exploit a supercomputer for large scale simulation activities to analyse experimental data on fusion plasmas, prepare scenarios for ITER operation, predict the performance of the ITER facilities and contribute to the DEMO Design.

  9. The New Progress of the Starry Sky Project of China

    Science.gov (United States)

    Wang, Xiaohua

    2015-08-01

    Since the 28th General Assembly of IAU, the SSPC team made new progress:1. Enhanced the function of the SSPC team-- Established the contact with IAU C50, IUCN Dark Skies Advisory Group, AWB and IDA,and undertakes the work of the IDA Beijing Chapter.-- Got supports from China’s National Astronomical Observatories, Beijing Planetarium, and Shanghai Science and Technology Museum.-- Signed cooperation agreements with Lighting Research Center, English Education Group and law Firm; formed the team force.2. Put forward a proposal to national top institutionThe SSPC submitted the first proposal about dark sky protection to the Chinese People’s Political Consultative Conference.3. Introduced the Criteria and Guideline of dark sky protectionThe SSPC team translated 8 documents of IDA, and provided a reference basis for Chinese dark sky protection.4. Actively establish dark sky places-- Plan a Dark Sky Reserve around Ali astronomical observatory (5,100m elevation) in Tibet. China’s Xinhua News Agency released the news.-- Combining with Hangcuo Lake, a National Natural Reserve and Scenic in Tibet, to plan and establish the Dark Sky Park.-- Cooperated with Shandong Longgang Tourism Group to construct the Dream Sky Theme Park in the suburbs of Jinan city.In the IYL 2015, the SSPC is getting further development:First, make dark sky protection enter National Ecological Strategy of “Beautiful China”. We call on: “Beautiful China” needs “Beautiful Night Sky” China should care the shared starry sky, and left this resource and heritage for children.Second, hold “Cosmic Light” exhibition in Shanghai Science and Technology Museum on August.Third, continue to establish Dark Sky Reserve, Park and Theme Park. We want to make these places become the bases of dark sky protection, astronomical education and ecological tourism, and develop into new cultural industry.Fourth, actively join international cooperation.Now, “Blue Sky, White Cloud and Starry Sky “have become

  10. Tritium effluent control project progress report, July--September 1975

    International Nuclear Information System (INIS)

    Kershner, C.J.; Bixel, J.C.

    1976-01-01

    In the Tritiated Liquid Waste Decontamination (Molecular Excitation) program, it was found that a 1/4'' Nd:YAG amplifier and a Faraday rotator isolator must be added to the oscillator to obtain the required power for the laser excitation experiments. The isotopic selectivity of the two-photon dissociation process was examined in more detail. The selectivity or ratio of HTO to H 2 O molecules that are photodissociated was found to be on the order of 25, which is inadequate for application to large-scale detritiation projects, e.g., nuclear fuel reprocessing plant waste. The selectivity is such that most of the laser energy is wasted in photodissociation of H 2 O. This obstacle may be overcome by pumping more vibrational energy into the HTO molecules. Various schemes towards this end are being considered. Extensive ab initio calculations on the potential surface for the hydrogen atom-water exchange reaction have been carried out. The energy barrier is calculated to be 38 1/2 kcal/mole, which implies that the exchange reaction T + H 2 O → HTO + H is at least 10 9 times slower than the exchange reaction T + H 2 → HT + H. Calculations were also performed on the analogous hydrogen atom-hydrogen fluoride exchange reaction. In lieu of other disposal methods a system using a General Electric UCT-1 regenerative cell has been assembled and tested. This system should be capable of decomposing pure water into hydrogen and oxygen gases. The tritium from this process can be purified and reused instead of buried. A preliminary economic evaluation was made of the HT/H 2 O catalytic exchange detritiation stripping process as it might apply to a 5 metric ton/day nuclear fuel reprocessing plant. A tritiated water shipping container has been designed and developed for use with the ERDA--DOT approved AL--MI secondary container

  11. Alignment of 1000 Genomes Project reads to reference assembly GRCh38.

    Science.gov (United States)

    Zheng-Bradley, Xiangqun; Streeter, Ian; Fairley, Susan; Richardson, David; Clarke, Laura; Flicek, Paul

    2017-07-01

    The 1000 Genomes Project produced more than 100 trillion basepairs of short read sequence from more than 2600 samples in 26 populations over a period of five years. In its final phase, the project released over 85 million genotyped and phased variants on human reference genome assembly GRCh37. An updated reference assembly, GRCh38, was released in late 2013, but there was insufficient time for the final phase of the project analysis to change to the new assembly. Although it is possible to lift the coordinates of the 1000 Genomes Project variants to the new assembly, this is a potentially error-prone process as coordinate remapping is most appropriate only for non-repetitive regions of the genome and those that did not see significant change between the two assemblies. It will also miss variants in any region that was newly added to GRCh38. Thus, to produce the highest quality variants and genotypes on GRCh38, the best strategy is to realign the reads and recall the variants based on the new alignment. As the first step of variant calling for the 1000 Genomes Project data, we have finished remapping all of the 1000 Genomes sequence reads to GRCh38 with alternative scaffold-aware BWA-MEM. The resulting alignments are available as CRAM, a reference-based sequence compression format. The data have been released on our FTP site and are also available from European Nucleotide Archive to facilitate researchers discovering variants on the primary sequences and alternative contigs of GRCh38. © The Authors 2017. Published by Oxford University Press.

  12. Genotype Imputation for Latinos Using the HapMap and 1000 Genomes Project Reference Panels

    Directory of Open Access Journals (Sweden)

    Xiaoyi eGao

    2012-06-01

    Full Text Available Genotype imputation is a vital tool in genome-wide association studies (GWAS and meta-analyses of multiple GWAS results. Imputation enables researchers to increase genomic coverage and to pool data generated using different genotyping platforms. HapMap samples are often employed as the reference panel. More recently, the 1000 Genomes Project resource is becoming the primary source for reference panels. Multiple GWAS and meta-analyses are targeting Latinos, the most populous and fastest growing minority group in the US. However, genotype imputation resources for Latinos are rather limited compared to individuals of European ancestry at present, largely because of the lack of good reference data. One choice of reference panel for Latinos is one derived from the population of Mexican individuals in Los Angeles contained in the HapMap Phase 3 project and the 1000 Genomes Project. However, a detailed evaluation of the quality of the imputed genotypes derived from the public reference panels has not yet been reported. Using simulation studies, the Illumina OmniExpress GWAS data from the Los Angles Latino Eye Study and the MACH software package, we evaluated the accuracy of genotype imputation in Latinos. Our results show that the 1000 Genomes Project AMR+CEU+YRI reference panel provides the highest imputation accuracy for Latinos, and that also including Asian samples in the panel can reduce imputation accuracy. We also provide the imputation accuracy for each autosomal chromosome using the 1000 Genomes Project panel for Latinos. Our results serve as a guide to future imputation-based analysis in Latinos.

  13. The 1000 Genomes Project: new opportunities for research and social challenges

    Science.gov (United States)

    2010-01-01

    The 1000 Genomes Project, an international collaboration, is sequencing the whole genome of approximately 2,000 individuals from different worldwide populations. The central goal of this project is to describe most of the genetic variation that occurs at a population frequency greater than 1%. The results of this project will allow scientists to identify genetic variation at an unprecedented degree of resolution and will also help improve the imputation methods for determining unobserved genetic variants that are not represented on current genotyping arrays. By identifying novel or rare functional genetic variants, researchers will be able to pinpoint disease-causing genes in genomic regions initially identified by association studies. This level of detailed sequence information will also improve our knowledge of the evolutionary processes and the genomic patterns that have shaped the human species as we know it today. The new data will also lay the foundation for future clinical applications, such as prediction of disease susceptibility and drug response. However, the forthcoming availability of whole genome sequences at affordable prices will raise ethical concerns and pose potential threats to individual privacy. Nevertheless, we believe that these potential risks are outweighed by the benefits in terms of diagnosis and research, so long as rigorous safeguards are kept in place through legislation that prevents discrimination on the basis of the results of genetic testing. PMID:20193048

  14. S.E.N.S.I.B. project. Progress report 2006

    International Nuclear Information System (INIS)

    2007-01-01

    This report presents the state of progress of all the studies which establish at present the S.E.N.S.I.B. project. For year 2006, the progress of the project is globally in compliance with the general schedule of realization of the S.E.N.S.I.B. project and with the perspectives announced in 2005. Factors of sensitivity were identified in diverse circles ( thematic studies) and methods and specific tools of the project are developed. 14 publications (reviews and congress) and 9 I.R.S.N. reports were produced. An international work group was launched to the I.R.S.N. initiative. The web site of S.E.N.S.I.B. on the I.R.S.N. scientific site was built. The S.E.N.S.I.B. project receives a financial participation of the Ademe. (N.C.)

  15. Genome-Wide Search for Host Association Factors during Ovine Progressive Pneumonia Virus Infection.

    Directory of Open Access Journals (Sweden)

    Jesse Thompson

    Full Text Available Ovine progressive pneumonia virus (OPPV is an important virus that causes serious diseases in sheep and goats with a prevalence of 36% in the USA. Although OPPV was discovered more than half of a century ago, little is known about the infection and pathogenesis of this virus. In this report, we used RNA-seq technology to conduct a genome-wide probe for cellular factors that are associated with OPPV infection. A total of approximately 22,000 goat host genes were detected of which 657 were found to have been significantly up-regulated and 889 down-regulated at 12 hours post-infection. In addition to previously known restriction factors from other viral infections, a number of factors which may be specific for OPPV infection were uncovered. The data from this RNA-seq study will be helpful in our understanding of OPPV infection, and also for further study in the prevention and intervention of this viral disease.

  16. Geothermal direct-heat utilization assistance. Quarterly project progress report, April--June 1993

    Energy Technology Data Exchange (ETDEWEB)

    Lienau, P.

    1993-06-01

    Technical assistance was provided to 60 requests from 19 states. R&D progress is reported on: evaluation of lineshaft turbine pump problems, geothermal district heating marketing strategy, and greenhouse peaking analysis. Two presentations and one tour were conducted, and three technical papers were prepared. The Geothermal Progress Monitor reported: USGS Forum on Mineral Resources, Renewable Energy Tax Credits Not Working as Congress Intended, Geothermal Industry Tells House Panel, Newberry Pilot Project, and Low-Temperature Geothermal Resources in Nevada.

  17. Functional food ingredients against colorectal cancer. An example project integrating functional genomics, nutrition and health

    NARCIS (Netherlands)

    Stierum, R.; Burgemeister, R.; Helvoort, van A.; Peijnenburg, A.; Schütze, K.; Seidelin, M.; Vang, O.; Ommen, van B.

    2001-01-01

    Functional Food Ingredients Against Colorectal Cancer is one of the first European Union funded Research Projects at the cross-road of functional genomics [comprising transcriptomics, the measurement of the expression of all messengers RNA (mRNAs) and proteomics, the measurement of expression/state

  18. The Human Genome Project and Eugenics: Identifying the Impact on Individuals with Mental Retardation.

    Science.gov (United States)

    Kuna, Jason

    2001-01-01

    This article explores the impact of the mapping work of the Human Genome Project on individuals with mental retardation and the negative effects of genetic testing. The potential to identify disabilities and the concept of eugenics are discussed, along with ethical issues surrounding potential genetic therapies. (Contains references.) (CR)

  19. Reflections on Mental Retardation and Eugenics, Old and New: Mensa and the Human Genome Project.

    Science.gov (United States)

    Smith, J. David

    1994-01-01

    This article addresses the moral and ethical issues of mental retardation and a continuing legacy of belief in eugenics. It discusses the involuntary sterilization of Carrie Buck in 1927, support for legalized killing of subnormal infants by 47% of respondents to a Mensa survey, and implications of the Human Genome Project for the field of mental…

  20. Democratizing Human Genome Project Information: A Model Program for Education, Information and Debate in Public Libraries.

    Science.gov (United States)

    Pollack, Miriam

    The "Mapping the Human Genome" project demonstrated that librarians can help whomever they serve in accessing information resources in the areas of biological and health information, whether it is the scientists who are developing the information or a member of the public who is using the information. Public libraries can guide library…

  1. From Mendel to the Human Genome Project: The Implications for Nurse Education.

    Science.gov (United States)

    Burton, Hilary; Stewart, Alison

    2003-01-01

    The Human Genome Project is brining new opportunities to predict and prevent diseases. Although pediatric nurses are the closest to these developments, most nurses will encounter genetic aspects of practice and must understand the basic science and its ethical, legal, and social dimensions. (Includes commentary by Peter Birchenall.) (SK)

  2. Ceramic Technology Project. Semiannual progress report for April 1993 through September 1993

    Energy Technology Data Exchange (ETDEWEB)

    1994-04-01

    The Ceramic Technology Project was originally developed by the Department of Energy`s Office of Transportation Systems (OTS) in Conservation and Renewable Energy. This project, part of the OTS`s Materials Development Program, was developed to meet the ceramic technology requirements of the OTS`s automotive technology programs. During the course of the Ceramic Technology Project, remarkable progress has been made in the development of reliable structural ceramics. However, further work is needed to reduce the cost of ceramics to facilitate their commercial introduction, especially in the highly cost-sensitive automotive market. The work described in this report is organized according to the following WBS project elements: Project Management and Coordination; Materials and Processing; Materials Design Methodology; Data Base and Life Prediction; and Technology Transfer. This report includes contributions from all currently active project participants. Separate abstracts were prepared for the 47 projects reported here.

  3. Demand-side management project for Tenaga Nasional Berhad. Progress report No. 4. Export trade information

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    This study, conducted by the California Energy Commission, was funded by the U.S. Trade and Development Agency. Progress Report No. 4 is divided into the following contents: April 6, 1994 Training Mission Delegation Itinerary; April 6, 1994 Training Mission Workshop Information Package; Letter to Chairman of Tenaga Nasional Berhad Requesting Demonstration Project Funding; Design/Build Construction Bid Proposal for Energy Efficient Electric Technology Demontration Project; Final Draft of Demand-Side Management Plan for Tenaga Nasional Berhad; Attachments.

  4. Getting the Word Out on the Human Genome Project: A Course for Physicians

    Energy Technology Data Exchange (ETDEWEB)

    Sara L. Tobin

    2004-09-29

    Our project, ''Getting the Word Out on the Human Genome Project: A Course for Physicians,'' presented educational goals to convey the power and promise of the Human Genome Program to a variety of professional, educational, and public audiences. Our initial goal was to provide practicing physicians with a comprehensive multimedia tool to update their skills in the genomic era. We therefore created the multimedia courseware, ''The New Genetics: Courseware for Physicians. Molecular Concepts, Applications, and Ramifications.'' However, as the project moved forward, several unanticipated audiences found the courseware to be useful for instruction and for self-education, so an additional edition of the courseware ''The New Genetics: Medicine and the Human Genome. Molecular Concepts, Applications, and Ramifications'' was published simultaneously with the physician version. At the time that both versions of the courseware were being completed, Stanford's Office of Technology Licensing opted not to commercialize the courseware and offered a license-back agreement if the authors founded a commercial business. The authors thus became closely involved in marketing and sales, and several thousand copies of the courseware have been sold. Surprisingly, the non-physician version has turned out to be more in demand, and this has led us in several new directions, most of which involve undergraduate education. These are discussed in detail in the Report.

  5. The European Renal Genome Project: An Integrated Approach Towards Understanding the Genetics of Kidney Development and Disease

    OpenAIRE

    Willnow, TE; Antignac, C; Brändli, AW; Christensen, EI; Cox, RD; Davidson, D; Davies, JA; Devuyst, O; Eichele, G; Hastie, ND; Verroust, PJ; Schedl, A; Meij, IC

    2005-01-01

    Rapid progress in genome research creates a wealth of information on the functional annotation of mammalian genome sequences. However, as we accumulate large amounts of scientific information we are facing problems of how to integrate and relate the data produced by various genomic approaches. Here, we propose the novel concept of an organ atlas where diverse data from expression maps to histological findings to mutant phenotypes can be queried, compared and visualized in the context of a thr...

  6. Appearance traits in fish farming: progress from classical genetics to genomics, providing insight into current and potential genetic improvement

    Directory of Open Access Journals (Sweden)

    Nelson eColihueque

    2014-08-01

    Full Text Available Appearance traits in fish, those external body characteristics that influence consumer acceptance at point of sale, have come to the forefront of commercial fish farming, as culture profitability is closely linked to management of these traits. Appearance traits comprise mainly body shape and skin pigmentation. Analysis of the genetic basis of these traits in different fish reveals significant genetic variation within populations, indicating potential for their genetic improvement. Work into ascertaining the minor or major genes underlying appearance traits for commercial fish is emerging, with substantial progress in model fish in terms of identifying genes that control body shape and skin colors. In this review, we describe research progress to date, especially with regard to commercial fish, and discuss genomic findings in model fish in order to better address the genetic basis of the traits. Given that appearance traits are important in commercial fish, the genomic information related to this issue promises to accelerate the selection process in coming years.

  7. Appearance traits in fish farming: progress from classical genetics to genomics, providing insight into current and potential genetic improvement

    Science.gov (United States)

    Colihueque, Nelson; Araneda, Cristian

    2014-01-01

    Appearance traits in fish, those external body characteristics that influence consumer acceptance at point of sale, have come to the forefront of commercial fish farming, as culture profitability is closely linked to management of these traits. Appearance traits comprise mainly body shape and skin pigmentation. Analysis of the genetic basis of these traits in different fish reveals significant genetic variation within populations, indicating potential for their genetic improvement. Work into ascertaining the minor or major genes underlying appearance traits for commercial fish is emerging, with substantial progress in model fish in terms of identifying genes that control body shape and skin colors. In this review, we describe research progress to date, especially with regard to commercial fish, and discuss genomic findings in model fish in order to better address the genetic basis of the traits. Given that appearance traits are important in commercial fish, the genomic information related to this issue promises to accelerate the selection process in coming years. PMID:25140172

  8. Identification of balanced chromosomal rearrangements previously unknown among participants in the 1000 Genomes Project: implications for interpretation of structural variation in genomes and the future of clinical cytogenetics.

    Science.gov (United States)

    Dong, Zirui; Wang, Huilin; Chen, Haixiao; Jiang, Hui; Yuan, Jianying; Yang, Zhenjun; Wang, Wen-Jing; Xu, Fengping; Guo, Xiaosen; Cao, Ye; Zhu, Zhenzhen; Geng, Chunyu; Cheung, Wan Chee; Kwok, Yvonne K; Yang, Huanming; Leung, Tak Yeung; Morton, Cynthia C; Cheung, Sau Wai; Choy, Kwong Wai

    2017-11-02

    PurposeRecent studies demonstrate that whole-genome sequencing enables detection of cryptic rearrangements in apparently balanced chromosomal rearrangements (also known as balanced chromosomal abnormalities, BCAs) previously identified by conventional cytogenetic methods. We aimed to assess our analytical tool for detecting BCAs in the 1000 Genomes Project without knowing which bands were affected.MethodsThe 1000 Genomes Project provides an unprecedented integrated map of structural variants in phenotypically normal subjects, but there is no information on potential inclusion of subjects with apparent BCAs akin to those traditionally detected in diagnostic cytogenetics laboratories. We applied our analytical tool to 1,166 genomes from the 1000 Genomes Project with sufficient physical coverage (8.25-fold).ResultsWith this approach, we detected four reciprocal balanced translocations and four inversions, ranging in size from 57.9 kb to 13.3 Mb, all of which were confirmed by cytogenetic methods and polymerase chain reaction studies. One of these DNAs has a subtle translocation that is not readily identified by chromosome analysis because of the similarity of the banding patterns and size of exchanged segments, and another results in disruption of all transcripts of an OMIM gene.ConclusionOur study demonstrates the extension of utilizing low-pass whole-genome sequencing for unbiased detection of BCAs including translocations and inversions previously unknown in the 1000 Genomes Project.GENETICS in MEDICINE advance online publication, 2 November 2017; doi:10.1038/gim.2017.170.

  9. Contracts for field projects and supporting research on enhanced oil recovery. Progress review No. 89

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-01

    Summaries are presented for the DOE contracts related to supported research for thermal recovery of petroleum, geoscience technology, and field demonstrations in high-priority reservoir classes. Data included for each project are: title, contract number, principal investigator, research organization, beginning date, expected completion date, amount of award, objectives of the research, and summary of technical progress.

  10. Earth BioGenome Project: Sequencing life for the future of life.

    Science.gov (United States)

    Lewin, Harris A; Robinson, Gene E; Kress, W John; Baker, William J; Coddington, Jonathan; Crandall, Keith A; Durbin, Richard; Edwards, Scott V; Forest, Félix; Gilbert, M Thomas P; Goldstein, Melissa M; Grigoriev, Igor V; Hackett, Kevin J; Haussler, David; Jarvis, Erich D; Johnson, Warren E; Patrinos, Aristides; Richards, Stephen; Castilla-Rubio, Juan Carlos; van Sluys, Marie-Anne; Soltis, Pamela S; Xu, Xun; Yang, Huanming; Zhang, Guojie

    2018-04-24

    Increasing our understanding of Earth's biodiversity and responsibly stewarding its resources are among the most crucial scientific and social challenges of the new millennium. These challenges require fundamental new knowledge of the organization, evolution, functions, and interactions among millions of the planet's organisms. Herein, we present a perspective on the Earth BioGenome Project (EBP), a moonshot for biology that aims to sequence, catalog, and characterize the genomes of all of Earth's eukaryotic biodiversity over a period of 10 years. The outcomes of the EBP will inform a broad range of major issues facing humanity, such as the impact of climate change on biodiversity, the conservation of endangered species and ecosystems, and the preservation and enhancement of ecosystem services. We describe hurdles that the project faces, including data-sharing policies that ensure a permanent, freely available resource for future scientific discovery while respecting access and benefit sharing guidelines of the Nagoya Protocol. We also describe scientific and organizational challenges in executing such an ambitious project, and the structure proposed to achieve the project's goals. The far-reaching potential benefits of creating an open digital repository of genomic information for life on Earth can be realized only by a coordinated international effort.

  11. Annual report on reactor safety research projects. Reporting period 2013. Progress report

    International Nuclear Information System (INIS)

    2013-01-01

    Within its competence for energy research the Federal Ministry of Economics and Technology (BMWi) sponsors research projects on the safety of nuclear power plants currently in operation. The objective of these projects is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS)mbH, by order of the BMWi, continuously issues information on the status of such research projects by publishing semi-annual and annual progress reports within the series of GRSF- Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are prepared in a standard form by the research organisations themselves as documentation of their progress in work. The progress reports are published by the Project Management Agency/Authority Support Division of GRS. The reports as of the year 2000 are available in the Internet-based information system on results and data of reactor safety research (http://www.grs-fbw.de). The compilation of the reports is classified according to the classification system ''Joint Safety Research Index (JSRI)''. The reports are arranged in sequence of their project numbers. It has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties. (orig.)

  12. Annual report on reactor safety research projects. Reporting period 2011. Progress report

    International Nuclear Information System (INIS)

    2011-01-01

    Within its competence for energy research the Federal Ministry of Economics and Technology (BMWi) sponsors research projects on the safety of nuclear power plants currently in operation. The objective of these projects is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS)mbH, by order of the BMWi, continuously issues information on the status of such research projects by publishing semi-annual and annual progress reports within the series of GRSF- Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are prepared in a standard form by the research organisations themselves as documentation of their progress in work. The progress reports are published by the Project Management Agency/Authority Support Division of GRS. The reports as of the year 2000 are available in the Internet-based information system on results and data of reactor safety research (http://www.grs-fbw.de). The compilation of the reports is classified according to the classification system ''Joint Safety Research Index (JSRI)''. The reports are arranged in sequence of their project numbers. It has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties. (orig.)

  13. Annual report on reactor safety research projects. Reporting period 2014. Progress report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2014-07-01

    Within its competence for energy research the Federal Ministry for Economic Affairs and Energy (BMWi) sponsors research projects on the safety of nuclear power plants currently in operation. The objective of these projects is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) gGmbH, by order of the BMWi, continuously issues information on the status of such research projects by publishing semi-annual and annual progress reports within the series of GRS-F-Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are prepared in a standard form by the research organisations themselves as documentation of their progress in work. The progress reports are published by the Project Management Agency/Authority Support Division of GRS. The reports as of the year 2000 are available in the lnternet-based information system on results and data of reactor safety research (http://www.grs-fbw.de). The compilation of the reports is classified according to the classification system ''Joint Safety Research Index (JSRI)''. The reports are arranged in sequence of their project numbers. lt has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties.

  14. Annual report on reactor safety research projects. Reporting period 2015. Progress report

    International Nuclear Information System (INIS)

    2015-01-01

    Within its competence for energy research the Federal Ministry for Economic Affairs and Energy (BMWi) sponsors research projects on the safety of nuclear power plants currently in operation. The objective of these projects is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft tor Anlagen- und Reaktorsicherheit (GRS) gGmbH, by order of the BMWi, continuously issues information on the status of such research projects by publishing semi-annual and annual progress reports within the series of GRS-F-Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are ·' prepared in a standard form by the research organisations themselves as documentation of their progress in work. The progress reports are published by the Project Management Agency/Authority Support Division of GRS. The reports as of the year 2000 are available in the lnternet-based information system on results and data of reactor safety research (http://www.grs-fbw.de). The compilation of the reports is classified according to the classification system ''Joint Safety Research Index (JSRI)''. The reports are arranged in sequence of their project numbers. it has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties.

  15. Environment, safety and health progress assessment of the Fernald Environmental Management Project (FEMP)

    International Nuclear Information System (INIS)

    1991-11-01

    This report documents the results of the Environment, Safety, and Health (ES ampersand H) Progress Assessment of the Fernald Environmental Management Project (FEMP), Fernald, Ohio, conducted from October 15 through October 25, 1991. The Secretary of Energy directed that small, focused, ES ampersand H Progress Assessments be performed as part of the continuing effort to institutionalize line management accountability and the self-assessment process in the areas of ES ampersand H. The FEMP assessment is the pilot assessment for this new program. The objectives for the FEMP ES ampersand H Progress Assessment were to assess: (1) how the FEMP has progressed since the 1989 Tiger Assessment; (2) how effectively the FEMP has corrected specific deficiencies and associated root causes identified by that team; and (3) whether the current organization, resources, and systems are sufficient to proactively manage ES ampersand H issues

  16. Environment, safety and health progress assessment of the Fernald Environmental Management Project (FEMP)

    Energy Technology Data Exchange (ETDEWEB)

    1991-11-01

    This report documents the results of the Environment, Safety, and Health (ES&H) Progress Assessment of the Fernald Environmental Management Project (FEMP), Fernald, Ohio, conducted from October 15 through October 25, 1991. The Secretary of Energy directed that small, focused, ES&H Progress Assessments be performed as part of the continuing effort to institutionalize line management accountability and the self-assessment process in the areas of ES&H. The FEMP assessment is the pilot assessment for this new program. The objectives for the FEMP ES&H Progress Assessment were to assess: (1) how the FEMP has progressed since the 1989 Tiger Assessment; (2) how effectively the FEMP has corrected specific deficiencies and associated root causes identified by that team; and (3) whether the current organization, resources, and systems are sufficient to proactively manage ES&H issues.

  17. Citrus sinensis annotation project (CAP): a comprehensive database for sweet orange genome.

    Science.gov (United States)

    Wang, Jia; Chen, Dijun; Lei, Yang; Chang, Ji-Wei; Hao, Bao-Hai; Xing, Feng; Li, Sen; Xu, Qiang; Deng, Xiu-Xin; Chen, Ling-Ling

    2014-01-01

    Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit and processed juice. We have sequenced the draft genome of a double-haploid sweet orange (C. sinensis cv. Valencia), and constructed the Citrus sinensis annotation project (CAP) to store and visualize the sequenced genomic and transcriptome data. CAP provides GBrowse-based organization of sweet orange genomic data, which integrates ab initio gene prediction, EST, RNA-seq and RNA-paired end tag (RNA-PET) evidence-based gene annotation. Furthermore, we provide a user-friendly web interface to show the predicted protein-protein interactions (PPIs) and metabolic pathways in sweet orange. CAP provides comprehensive information beneficial to the researchers of sweet orange and other woody plants, which is freely available at http://citrus.hzau.edu.cn/.

  18. Progress reports on SCWR-related development projects from Chinese universities for FY2008-2009

    International Nuclear Information System (INIS)

    Leung, L.K.H.

    2010-02-01

    Canada is participating in the international cooperative forum on system research for two designs (supercritical water-cooled reactor, SCWR, and Very High Temperature Reactor, VHTR) of the Gen-IV nuclear reactor. The forum is referred to as the Generation-IV International Forum (or GIF). The Canadian effort focuses mainly on the SCWR. Among various GIF participants, Canada is the leader of this design and has interest mainly on the pressure-tube type reactor, which is a natural extension of the existing CANDU reactor. Several critical research areas (such as material, chemistry, thermalhydraulics, instability, critical flow, etc.) have been identified in the system-research plan for supporting the SCWR design. Collaborative projects have been established between AECL and universities in China to expedite the CANDU SCWR design. These projects focus on research areas beyond the current scope of the AECL and the NSERC/NRCan/AECL collaborative research and development (CRD) project. AECL supports these projects directly and is contributing (in-kind) the results and findings to the Canadian national program. The collaboration between AECL and Chinese universities began in 2007 July. Most projects cover the duration of three years. The Chinese universities submit their annual progress reports each year prior to the project renewal. The objective of this report is to summarize the progress on collaborative projects between AECL and Chinese universities (namely the Tsinghua University, Shanghai Jiaotong University, and Xi'an Jiaotong University) over the duration of 2008 July to 2009 June. (author)

  19. Progress and status of the international project on innovative nuclear reactors and fuel cycles (INPRO) - 5182

    International Nuclear Information System (INIS)

    Ponomarev, A.; Fesenko, G.; Grigoriev, F.G.; Korinny, A.; Phillips, J.R.; Rho, K.

    2015-01-01

    The IAEA's International Project on Innovative Nuclear Reactors and Fuel Cycles (INPRO) was established in 2000 through IAEA General Conference resolution. INPRO cooperates with Member States to ensure that sustainable nuclear energy is available to help meet the energy needs of the 21. century. INPRO membership has grown to 41 members and 16 observers. The paper presents the current prospectus of the INPRO programme and details the most recent achievements in the following 7 projects: 1) the GAINS project (Global Architecture of Innovative Nuclear Energy Systems with thermal and fast reactors and a closed nuclear fuel cycle); 2) the SYNERGIES project applies and amends the analytical framework developed in GAINS project to examine more specifically the various forms of regional collaboration among nuclear energy suppliers and users; 3) the KIND project (Key Indicators for Innovative Nuclear Energy Systems) has the objective of developing guidance on the evaluation on innovative nuclear technologies; 4) the ROADMAPS project addresses several possible stages toward nuclear energy sustainability; 5) the RISC project aims at demonstrating that the evolution of safety requirements and technical innovations provide continual progress towards the avoidance of evacuation measures outside NPP sites in case of severe accidents; 6) the FANES project has the objective of carrying out feasibility analyses of advanced and innovative fuels for different reactor systems; and 7) the WIRAF project aims at identifying problematic waste from innovative reactor designs and corresponding nuclear fuel cycles

  20. International Project on Innovative Nuclear Reactors and Fuel Cycles (INPRO). 2008 progress report

    International Nuclear Information System (INIS)

    2009-02-01

    The purpose of the work is to review the progress of the IAEA international project for innovative reactors and fuel cycle technologies (INPRO). The publication reports about the recognition of INPRO and on general Information on INPRO, its strengths, memberships, collaboration with other international initiatives, the INPRO organization and management and the history of INPRO. The section on the progress of INPRO in 2008 contains task 1: INPRO Methodology, task 2: Assessment Studies, task 3: Nuclear Energy Visions for the 21st Century, task 4: Infrastructure and Institutional Innovation, task 5: Common User Considerations and task 6: Collaborative Projects. Conclusions and New Trends are followed by a bibliography. Annex I deals with the INPRO project management in 2008 and Annex II provides a selection of photographs from 2008. Finally a list of acronyms is provided

  1. Ground-water monitoring compliance projects for Hanford Site facilities: Annual progress report for 1987

    International Nuclear Information System (INIS)

    Hall, S.H.

    1988-09-01

    This report describes progress during 1987 of five Hanford Site ground water monitoring projects. Four of these projects are being conducted according to regulations based on the federal Resource Conservation and Recovery Act of 1976 and the state Hazardous Waste Management Act. The fifth project is being conducted according to regulations based on the state Solid Waste Management Act. The five projects discussed herein are: 300 Area Process Trenches; 183-H Solar Evaporation Basins; 200 Areas Low-Level Burial Grounds; Nonradioactive Dangerous Waste Landfill; Solid Waste Landfill. For each of the projects, there are included, as applicable, discussions of monitoring well installations, water-table measurements, background and/or downgradient water quality and results of chemical analysis, and extent and rate of movement of contaminant plumes. 14 refs., 30 figs., 13 tabs

  2. ARCHER Project: Progress on Material and component activities for the Advanced High Temperature Reactor

    International Nuclear Information System (INIS)

    Buckthorpe, D.E.

    2014-01-01

    The ARCHER (Advanced High-Temperature Reactors for Cogeneration of Heat and Electricity R&D) integrated project is a four year project which was started in 2011 as part of the European Commission 7th Framework Programme (FP7) to perform High Temperature Reactor technology R&D in support of reactor demonstration. The project consortium encompasses conventional and Nuclear Industry, Utilities, Technical Support Organizations, Research & Development Organizations and Academia. The activities involved contribute to the Generation IV (GIF) International Forum and collaborate with related projects in the US, China, Japan, and the Republic of Korea in cooperation with IAEA and ISTC. This paper addresses the progress of the work on ARCHER materials and component activities since the start of the project and underlines some of the main conclusions reached. (author)

  3. Sequence Analysis and Characterization of Active Human Alu Subfamilies Based on the 1000 Genomes Pilot Project.

    Science.gov (United States)

    Konkel, Miriam K; Walker, Jerilyn A; Hotard, Ashley B; Ranck, Megan C; Fontenot, Catherine C; Storer, Jessica; Stewart, Chip; Marth, Gabor T; Batzer, Mark A

    2015-08-29

    The goal of the 1000 Genomes Consortium is to characterize human genome structural variation (SV), including forms of copy number variations such as deletions, duplications, and insertions. Mobile element insertions, particularly Alu elements, are major contributors to genomic SV among humans. During the pilot phase of the project we experimentally validated 645 (611 intergenic and 34 exon targeted) polymorphic "young" Alu insertion events, absent from the human reference genome. Here, we report high resolution sequencing of 343 (322 unique) recent Alu insertion events, along with their respective target site duplications, precise genomic breakpoint coordinates, subfamily assignment, percent divergence, and estimated A-rich tail lengths. All the sequenced Alu loci were derived from the AluY lineage with no evidence of retrotransposition activity involving older Alu families (e.g., AluJ and AluS). AluYa5 is currently the most active Alu subfamily in the human lineage, followed by AluYb8, and many others including three newly identified subfamilies we have termed AluYb7a3, AluYb8b1, and AluYa4a1. This report provides the structural details of 322 unique Alu variants from individual human genomes collectively adding about 100 kb of genomic variation. Many Alu subfamilies are currently active in human populations, including a surprising level of AluY retrotransposition. Human Alu subfamilies exhibit continuous evolution with potential drivers sprouting new Alu lineages. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  4. Perspectives from the Avian Phylogenomics Project: Questions that Can Be Answered with Sequencing All Genomes of a Vertebrate Class.

    Science.gov (United States)

    Jarvis, Erich D

    2016-01-01

    The rapid pace of advances in genome technology, with concomitant reductions in cost, makes it feasible that one day in our lifetime we will have available extant genomes of entire classes of species, including vertebrates. I recently helped cocoordinate the large-scale Avian Phylogenomics Project, which collected and sequenced genomes of 48 bird species representing most currently classified orders to address a range of questions in phylogenomics and comparative genomics. The consortium was able to answer questions not previously possible with just a few genomes. This success spurred on the creation of a project to sequence the genomes of at least one individual of all extant ∼10,500 bird species. The initiation of this project has led us to consider what questions now impossible to answer could be answered with all genomes, and could drive new questions now unimaginable. These include the generation of a highly resolved family tree of extant species, genome-wide association studies across species to identify genetic substrates of many complex traits, redefinition of species and the species concept, reconstruction of the genomes of common ancestors, and generation of new computational tools to address these questions. Here I present visions for the future by posing and answering questions regarding what scientists could potentially do with available genomes of an entire vertebrate class.

  5. Field of genes: the politics of science and identity in the Estonian Genome Project.

    Science.gov (United States)

    Fletcher, Amy L

    2004-04-01

    This case study of the Estonian Genome Project (EGP) analyses the Estonian policy decision to construct a national human gene bank. Drawing upon qualitative data from newspaper articles and public policy documents, it focuses on how proponents use discourse to link the EGP to the broader political goal of securing Estonia's position within the Western/European scientific and cultural space. This dominant narrative is then situated within the analytical notion of the "brand state", which raises potentially negative political consequences for this type of market-driven genomic research. Considered against the increasing number of countries engaging in gene bank and/or gene database projects, this analysis of Estonia elucidates issues that cross national boundaries, while also illuminating factors specific to this small, post-Soviet state as it enters the global biocybernetic economy.

  6. The MedSeq Project: a randomized trial of integrating whole genome sequencing into clinical medicine.

    Science.gov (United States)

    Vassy, Jason L; Lautenbach, Denise M; McLaughlin, Heather M; Kong, Sek Won; Christensen, Kurt D; Krier, Joel; Kohane, Isaac S; Feuerman, Lindsay Z; Blumenthal-Barby, Jennifer; Roberts, J Scott; Lehmann, Lisa Soleymani; Ho, Carolyn Y; Ubel, Peter A; MacRae, Calum A; Seidman, Christine E; Murray, Michael F; McGuire, Amy L; Rehm, Heidi L; Green, Robert C

    2014-03-20

    Whole genome sequencing (WGS) is already being used in certain clinical and research settings, but its impact on patient well-being, health-care utilization, and clinical decision-making remains largely unstudied. It is also unknown how best to communicate sequencing results to physicians and patients to improve health. We describe the design of the MedSeq Project: the first randomized trials of WGS in clinical care. This pair of randomized controlled trials compares WGS to standard of care in two clinical contexts: (a) disease-specific genomic medicine in a cardiomyopathy clinic and (b) general genomic medicine in primary care. We are recruiting 8 to 12 cardiologists, 8 to 12 primary care physicians, and approximately 200 of their patients. Patient participants in both the cardiology and primary care trials are randomly assigned to receive a family history assessment with or without WGS. Our laboratory delivers a genome report to physician participants that balances the needs to enhance understandability of genomic information and to convey its complexity. We provide an educational curriculum for physician participants and offer them a hotline to genetics professionals for guidance in interpreting and managing their patients' genome reports. Using varied data sources, including surveys, semi-structured interviews, and review of clinical data, we measure the attitudes, behaviors and outcomes of physician and patient participants at multiple time points before and after the disclosure of these results. The impact of emerging sequencing technologies on patient care is unclear. We have designed a process of interpreting WGS results and delivering them to physicians in a way that anticipates how we envision genomic medicine will evolve in the near future. That is, our WGS report provides clinically relevant information while communicating the complexity and uncertainty of WGS results to physicians and, through physicians, to their patients. This project will not only

  7. Shippingport Station Decommissioning Project: FYs 1984-1985 annual progress report, October 1, 1983 through September 30, 1985

    International Nuclear Information System (INIS)

    1987-01-01

    This report presents progress on the Shippingport Station Decommissioning Project for FYs 1984-85. There are three main topics: project management, decommissioning project activities, and issues of concern. The project purpose is demonstration of nuclear plant decommissioning and dismantlement operations in an environment of current industry practices. 8 refs., 8 figs., 2 tabs

  8. Public trust and 'ethics review' as a commodity: the case of Genomics England Limited and the UK's 100,000 genomes project.

    Science.gov (United States)

    Samuel, Gabrielle Natalie; Farsides, Bobbie

    2018-06-01

    The UK Chief Medical Officer's 2016 Annual Report, Generation Genome, focused on a vision to fully integrate genomics into all aspects of the UK's National Health Service (NHS). This process of integration, which has now already begun, raises a wide range of social and ethical concerns, many of which were discussed in the final Chapter of the report. This paper explores how the UK's 100,000 Genomes Project (100 kGP)-the catalyst for Generation Genome, and for bringing genomics into the NHS-is negotiating these ethical concerns. The UK's 100 kGP, promoted and delivered by Genomics England Limited (GEL), is an innovative venture aiming to sequence 100,000 genomes from NHS patients who have a rare disease, cancer, or an infectious disease. GEL has emphasised the importance of ethical governance and decision-making. However, some sociological critique argues that biomedical/technological organisations presenting themselves as 'ethical' entities do not necessarily reflect a space within which moral thinking occurs. Rather, the 'ethical work' conducted (and displayed) by organisations is more strategic, relating to the politics of the organisation and the need to build public confidence. We set out to explore whether GEL's ethical framework was reflective of this critique, and what this tells us more broadly about how genomics is being integrated into the NHS in response to the ethical and social concerns raised in Generation Genome. We do this by drawing on a series of 20 interviews with individuals associated with or working at GEL.

  9. Progress report for project modeling Arctic barrier island-lagoon system response to projected Arctic warming

    Science.gov (United States)

    Erikson, Li H.; Gibbs, Ann E.; Richmond, Bruce M.; Storlazzi, Curt; B.M. Jones,

    2012-01-01

    Changes in Arctic coastal ecosystems in response to global warming may be some of the most severe on the planet. A better understanding and analysis of the rates at which these changes are expected to occur over the coming decades is crucial in order to delineate high-priority areas that are likely to be affected by climate changes. In this study we investigate the likelihood of changes to habitat-supporting barrier island – lagoon systems in response to projected changes in atmospheric and oceanographic forcing associated with Arctic warming. To better understand the relative importance of processes responsible for the current and future coastal landscape, key parameters related to increasing arctic temperatures are investigated and used to establish boundary conditions for models that simulate barrier island migration and inundation of deltaic deposits and low-lying tundra. The modeling effort investigates the dominance and relative importance of physical processes shaping the modern Arctic coastline as well as decadal responses due to projected conditions out to the year 2100.

  10. Agency for Nuclear Projects/Nuclear Waste Project Office final progress report

    International Nuclear Information System (INIS)

    1992-01-01

    The Nevada Agency for Nuclear Projects/Nuclear Waste Project Office (NWPO) was formally established by Executive Policy in 1983 following passage of the federal Nuclear Waste Policy Act of 1982 (Act). That Act provides for the systematic siting, construction, operation, and closure of high-level radioactive defense and research by-products and other forms of high-level radioactive waste from around the country which will be stored at such repositories. In 1985 the Nevada legislature formally established the NWPO as a distinct and statutorily authorized agency to provide support to the Governor and State Legislature on matters concerning the high-level nuclear waste programs. The NWPO utilized a small, central staff supplemented by contractual services for needed technical and specialized expertise in order to provide high quality oversight and monitoring of federal activities, to conduct necessary independent studies, and to avoid unnecessary duplication of efforts. This report summarizes the results of this ongoing program to ensure that risks to the environment and to human safety are minimized. It includes findings in the areas of hydrogeology, geology, quality assurance activities, repository engineering, legislature participation, socioeconomic affects, risk assessments, monitoring programs, public information dissemination, and transportation activities. The bulk of the reporting deals with the Yucca Mountain facility

  11. Whole-genome and Transcriptome Sequencing of Prostate Cancer Identify New Genetic Alterations Driving Disease Progression

    DEFF Research Database (Denmark)

    Ren, Shancheng; Wei, Gong-Hong; Liu, Dongbing

    2018-01-01

    BACKGROUND: Global disparities in prostate cancer (PCa) incidence highlight the urgent need to identify genomic abnormalities in prostate tumors in different ethnic populations including Asian men. OBJECTIVE: To systematically explore the genomic complexity and define disease-driven genetic......-scale and comprehensive genomic data of prostate cancer from Asian population. Identification of these genetic alterations may help advance prostate cancer diagnosis, prognosis, and treatment....... alterations in PCa. DESIGN, SETTING, AND PARTICIPANTS: The study sequenced whole-genome and transcriptome of tumor-benign paired tissues from 65 treatment-naive Chinese PCa patients. Subsequent targeted deep sequencing of 293 PCa-relevant genes was performed in another cohort of 145 prostate tumors. OUTCOME...

  12. High participation rate among 25 721 patients with broad age range in a hospital-based research project involving whole-genome sequencing - the Lausanne Institutional Biobank.

    Science.gov (United States)

    Bochud, Murielle; Currat, Christine; Chapatte, Laurence; Roth, Cindy; Mooser, Vincent

    2017-10-24

    We aimed to evaluate the interest of adult inpatients and selected outpatients in engaging in a large, real-life, hospital-based, genomic medicine research project and in receiving clinically actionable incidental findings. Within the framework of the cross-sectional Institutional Biobank of Lausanne, Switzerland, a total of 25721 patients of the CHUV University Hospital were systematically invited to grant researchers access to their biomedical data and to donate blood for future analyses, including whole-genome sequencing. Multivariable logistic regression analysis was used to identify personal factors, including age, gender, religion, ethnicity, citizenship, education level and mode of admission, associated with willingness to participate in this genomic research project and with interest in receiving clinically actionable incidental findings. The overall participation rate was 79% (20343/25721). Participation rate declined progressively with age, averaging 83%, 75%, 67% and 62% in patients aged rate, but not with higher willingness to receive incidental findings within the population who had agreed to participate. A large proportion of adult patients, even among the elderly, are willing to actively participate and receive incidental findings in this systematic hospital-based precision and genomic medicine research program with broad consent.

  13. Low-Cost Solar Array Project. Progress report 14, August 1979-December 1979 and proceedings of the 14th Project Integration Meeting

    Energy Technology Data Exchange (ETDEWEB)

    1980-01-01

    Progress made by the Low-Cost Solar Array Project during the period August through November 1979, is described. Progress on project analysis and integration; technology development in silicon material, large-area sheet silicon, and encapsulation; production process and equipment development; engineering, and operations, and the steps taken to integrate these efforts are detailed. A report on the Project Integration Meeting held December 5-6, 1979, including copies of the visual materials used, is presented.

  14. Ethical challenges and innovations in the dissemination of genomic data: the experience of the PERSPECTIVE project

    Directory of Open Access Journals (Sweden)

    Lévesque E

    2015-08-01

    Full Text Available Emmanuelle Lévesque,1 Bartha Maria Knoppers,1 Jacques Simard,2 1Department of Human Genetics, Centre for Genomics and Policy, McGill University, Montréal, 2Genomics Centre, CHU de Québec Research Center, Department of Molecular Medicine, Laval University, Québec City, QC, Canada Abstract: The importance of making genomic data available for future research is now widely recognized among the scientific community and policymakers. In this era of shared responsibility for data dissemination, improved patient care through research depends on the development of powerful and secure data-sharing systems. As part of the concerted effort to share research resources, the project entitled Personalized Risk Stratification for Prevention and Early Detection of Breast Cancer (PERSPECTIVE makes effective data sharing through the development of a data-sharing framework, one of its goals. The secondary uses of data from PERSPECTIVE for future research promise to enhance our knowledge of breast cancer etiologies without duplicating data-gathering efforts. Despite its benefit for research, we recognize the ethical challenges of data sharing on the local, national, and international levels. The effective management of ethical approvals for projects spanning across jurisdictions, the return of results to research participants, and research incentives and recognition for data production, are but a few pressing issues that need to be properly addressed. We discuss how we managed these issues and suggest how ongoing innovations might help to facilitate data sharing in future genomic research projects. Keywords: data sharing, research ethics, cancer

  15. Hunting for genes for hypertension: the Millennium Genome Project for Hypertension.

    Science.gov (United States)

    Tabara, Yasuharu; Kohara, Katsuhiko; Miki, Tetsuro

    2012-06-01

    The Millennium Genome Project for Hypertension was started in 2000 to identify genetic variants conferring susceptibility to hypertension, with the aim of furthering the understanding of the pathogenesis of this condition and realizing genome-based personalized medical care. Two different approaches were launched, genome-wide association analysis using single-nucleotide polymorphisms (SNPs) and microsatellite markers, and systematic candidate gene analysis, under the hypothesis that common variants have an important role in the etiology of common diseases. These multilateral approaches identified ATP2B1 as a gene responsible for hypertension in not only Japanese but also Caucasians. The high blood pressure susceptibility conferred by certain alleles of ATP2B1 has been widely replicated in various populations. Ex vivo mRNA expression analysis in umbilical artery smooth muscle cells indicated that reduced expression of this gene associated with the risk allele may be an underlying mechanism relating the ATP2B1 variant to hypertension. However, the effect size of a SNP was too small to clarify the entire picture of the genetic basis of hypertension. Further, dense genome analysis with accurate phenotype data may be required.

  16. Management of water hyacinth. A CSC/UNEP project. Progress report

    International Nuclear Information System (INIS)

    1981-05-01

    The water hyacinth project was initially proposed at the Regional Workshop on Rural Technology held at Dacca in January 1978. In November 1978, national coordinators met at New Delhi and outlined the project in detail as reported in CSC(79)RT-4. The meeting was attended by delegates from Bangladesh, Egypt, Guyana, India, Malaysia, Papua New Guinea, Sri Lanka, Commonwealth Science Council and the United Nations Environment Programme. Following this a proposal was submitted to UNEP seeking funding support to meet the external cost component of the project. This support was subsequently granted. The project aims to achieve an integrated approach towards managing water hyacinth. The underlying intention was that management would cover both eradication of the plant as well as making productive use of it when possible. Productive uses envisaged include biogas synthesis, production of papers and boards and as a source of proteins. Another interesting possibility is the use of the plant to control industrial as well as domestic water pollution . All these were detailed in a three and a half year time plan. The project had its first review meeting in June 1979 in Papua New Guinea. The major intention of this meeting was to examine status reports from each country in an attempt to quantify the problem caused by water hyacinth and assess the work plan in relation to this. The report of this meeting has been published as CSC(79)RT-5. At this meeting Papua New Guinea decided to withdraw from this project as water hyacinth was not regarded as a severe problem. The use of dugong as a control agent was not recommended by Papua New Guinea. In April 1980 an interim review meeting attended by the Regional Coordinator and representatives of UNEP and CSC was held in London where, based on the progress made in the participating countries, activities and time schedules were refined and sharpened (CSC (80)RT-16). It look some time to resolve the external funding question . It was

  17. Identification of genetic variants associated with Huntington's disease progression: a genome-wide association study

    OpenAIRE

    Hensman Moss, Davina J; Pardinas, Antonio; Langbehn, Douglas; Lo, Kitty; Leavitt, Blair R; Roos, Raymund; Durr, Alexandra; Mead, Simon; Holmans, Peter; Jones, Lesley; Tabrizi, Sarah J; Coleman, A; Santos, R Dar; Decolongon, J; Sturrock, A

    2017-01-01

    Background\\ud \\ud Huntington's disease is caused by a CAG repeat expansion in the huntingtin gene, HTT. Age at onset has been used as a quantitative phenotype in genetic analysis looking for Huntington's disease modifiers, but is hard to define and not always available. Therefore, we aimed to generate a novel measure of disease progression and to identify genetic markers associated with this progression measure.\\ud \\ud Methods\\ud \\ud We generated a progression score on the basis of principal ...

  18. Characterization of apparently balanced chromosomal rearrangements from the developmental genome anatomy project.

    Science.gov (United States)

    Higgins, Anne W; Alkuraya, Fowzan S; Bosco, Amy F; Brown, Kerry K; Bruns, Gail A P; Donovan, Diana J; Eisenman, Robert; Fan, Yanli; Farra, Chantal G; Ferguson, Heather L; Gusella, James F; Harris, David J; Herrick, Steven R; Kelly, Chantal; Kim, Hyung-Goo; Kishikawa, Shotaro; Korf, Bruce R; Kulkarni, Shashikant; Lally, Eric; Leach, Natalia T; Lemyre, Emma; Lewis, Janine; Ligon, Azra H; Lu, Weining; Maas, Richard L; MacDonald, Marcy E; Moore, Steven D P; Peters, Roxanna E; Quade, Bradley J; Quintero-Rivera, Fabiola; Saadi, Irfan; Shen, Yiping; Shendure, Jay; Williamson, Robin E; Morton, Cynthia C

    2008-03-01

    Apparently balanced chromosomal rearrangements in individuals with major congenital anomalies represent natural experiments of gene disruption and dysregulation. These individuals can be studied to identify novel genes critical in human development and to annotate further the function of known genes. Identification and characterization of these genes is the goal of the Developmental Genome Anatomy Project (DGAP). DGAP is a multidisciplinary effort that leverages the recent advances resulting from the Human Genome Project to increase our understanding of birth defects and the process of human development. Clinically significant phenotypes of individuals enrolled in DGAP are varied and, in most cases, involve multiple organ systems. Study of these individuals' chromosomal rearrangements has resulted in the mapping of 77 breakpoints from 40 chromosomal rearrangements by FISH with BACs and fosmids, array CGH, Southern-blot hybridization, MLPA, RT-PCR, and suppression PCR. Eighteen chromosomal breakpoints have been cloned and sequenced. Unsuspected genomic imbalances and cryptic rearrangements were detected, but less frequently than has been reported previously. Chromosomal rearrangements, both balanced and unbalanced, in individuals with multiple congenital anomalies continue to be a valuable resource for gene discovery and annotation.

  19. Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes.

    Science.gov (United States)

    Naduthodi, Mihris Ibnu Saleem; Barbosa, Maria J; van der Oost, John

    2018-02-03

    The carbon footprint caused by unsustainable development and its environmental and economic impact has become a major concern in the past few decades. Photosynthetic microbes such as microalgae and cyanobacteria are capable of accumulating value-added compounds from carbon dioxide, and have been regarded as environmentally friendly alternatives to reduce the usage of fossil fuels, thereby contributing to reducing the carbon footprint. This light-driven generation of green chemicals and biofuels has triggered the research for metabolic engineering of these photosynthetic microbes. CRISPR-Cas systems are successfully implemented across a wide range of prokaryotic and eukaryotic species for efficient genome editing. However, the inception of this genome editing tool in microalgal and cyanobacterial species took off rather slowly due to various complications. In this review, we elaborate on the established CRISPR-Cas based genome editing in various microalgal and cyanobacterial species. The complications associated with CRISPR-Cas based genome editing in these species are addressed along with possible strategies to overcome these issues. It is anticipated that in the near future this will result in improving and expanding the microalgal and cyanobacterial genome engineering toolbox. © 2018 The Authors. Biotechnology Journal Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  20. Genome-wide alterations of the DNA replication program during tumor progression

    Science.gov (United States)

    Arneodo, A.; Goldar, A.; Argoul, F.; Hyrien, O.; Audit, B.

    2016-08-01

    Oncogenic stress is a major driving force in the early stages of cancer development. Recent experimental findings reveal that, in precancerous lesions and cancers, activated oncogenes may induce stalling and dissociation of DNA replication forks resulting in DNA damage. Replication timing is emerging as an important epigenetic feature that recapitulates several genomic, epigenetic and functional specificities of even closely related cell types. There is increasing evidence that chromosome rearrangements, the hallmark of many cancer genomes, are intimately associated with the DNA replication program and that epigenetic replication timing changes often precede chromosomic rearrangements. The recent development of a novel methodology to map replication fork polarity using deep sequencing of Okazaki fragments has provided new and complementary genome-wide replication profiling data. We review the results of a wavelet-based multi-scale analysis of genomic and epigenetic data including replication profiles along human chromosomes. These results provide new insight into the spatio-temporal replication program and its dynamics during differentiation. Here our goal is to bring to cancer research, the experimental protocols and computational methodologies for replication program profiling, and also the modeling of the spatio-temporal replication program. To illustrate our purpose, we report very preliminary results obtained for the chronic myelogeneous leukemia, the archetype model of cancer. Finally, we discuss promising perspectives on using genome-wide DNA replication profiling as a novel efficient tool for cancer diagnosis, prognosis and personalized treatment.

  1. Progress Report 16 for the period April-September 1980, and the proceedings of the 16th Project Integration Meeting

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, R.R.

    1980-01-01

    Progress made by the Low-Cost Solar Array Project during the period April to September 1980, is reported in detail. Progress on project analysis and integration; technology development in silicon material, large-area silicon sheet and encapsulation; production process and equipment development; engineering, and operations is described. A report on, and copies of visual presentations made at, the Project Integration Meeting held September 24 and 25, 1980 are included.

  2. One System Integrated Project Team Progress in Coordinating Hanford Tank Farms and the Waste Treatment Plant

    International Nuclear Information System (INIS)

    Skwarek, Raymond J.; Harp, Ben J.; Duncan, Garth M.

    2013-01-01

    The One System Integrated Project Team (IPT) was formed at the Hanford Site in late 2011 as a way to improve coordination and itegration between the Hanford Tank Waste Treatment and Immobilization Plant (WTP) and the Tank Operations Contractor (TOC) on interfaces between the two projects, and to eliminate duplication and exploit opportunities for synergy. The IPT is composed of jointly staffed groups that work on technical issues of mutal interest, front-end design and project definition, nuclear safety, plant engineering system integration, commissioning, planning and scheduling, and environmental, safety, health and quality (ESH&Q) areas. In the past year important progress has been made in a number of areas as the organization has matured and additional opportunities have been identified. Areas covered in this paper include: Support for development of the Office of Envirnmental Management (EM) framework document to progress the Office of River Protection's (ORP) River Protection Project (RPP) mission; Stewardship of the RPP flowsheet; Collaboration with Savannah River Site (SRS), Savannah River National Laboratory (SRNL), and Pacific Northwest National Laboratory (PNNL); Operations programs integration; and, Further development of the waste acceptance criteria

  3. White Matter Lesion Progression: Genome-Wide Search for Genetic Influences

    NARCIS (Netherlands)

    E. Hofer (Edith); M. Cavalieri (Margherita); J.C. Bis (Joshua); C. DeCarli (Charles); M. Fornage (Myriam); S. Sigurdsson (Sigurdur); V. Srikanth (Velandai); S. Trompet (Stella); B.F.J. Verhaaren (Benjamin); C. Wolf (Christiane); Q. Yang (Qiong Fang); H.H.H. Adams (Hieab); P. Amouyel (Philippe); A. Beiser (Alexa); B.M. Buckley (Brendan M.); M. Callisaya (Michele); G. Chauhan (Ganesh); A.J.M. De Craen (Anton J. M.); C. Dufouil (Carole); C.M. van Duijn (Cornelia); I. Ford; P. Freudenberger (Paul); R.F. Gottesman (Rebecca); V. Gudnason (Vilmundur); G. Heiss (Gerardo); A. Hofman (Albert); T. Lumley (Thomas); O. Martinez (Oliver); B. Mazoyer (Bernard); C. Moran (Chris); W.J. Niessen (Wiro); T.G. Phan (Thanh); B.M. Psaty (Bruce); C.L. Satizabal (Claudia L.); N. Sattar (Naveed); S. Schilling (Sabrina); D.K. Shibata (Dean); P.E. Slagboom (Eline); G.D. Smith; D.J. Stott (David. J.); K.D. Taylor (Kent); R. Thomson (Russell); A.M. Töglhofer (Anna Maria); C. Tzourio (Christophe); M.A. van Buchem (Mark); J. Wang (Jing); R.G.J. Westendorp (Rudi); B. Gwen Windham; M.W. Vernooij (Meike); A.P. Zijdenbos; R.J. Beare (Richard); S. Debette (Stéphanie); M.A. Ikram (Arfan); J.W. Jukema (Jan Wouter); L.J. Launer (Lenore); W.T. Longstreth Jr; T.H. Mosley (Thomas H.); S. Seshadri (Sudha); R. Schmidt (Reinhold); R. Schmidt (Reinhold)

    2015-01-01

    textabstractBackground and Purpose-White matter lesion (WML) progression on magnetic resonance imaging is related to cognitive decline and stroke, but its determinants besides baseline WML burden are largely unknown. Here, we estimated heritability of WML progression, and sought common genetic

  4. [Research progress in neuropsychopharmacology updated for the post-genomic era].

    Science.gov (United States)

    Nakanishi, Toru

    2009-11-01

    Neuropsychopharmacological research in the post genomic (genomic sequence) era has been developing rapidly through the use of novel techniques including DNA chips. We have applied these techniques to investigate the anti-tumor effect of NSAIDs, isolate novel genes specifically expressed in rheumatoid arthritis, and analyze gene expression profiles in mesenchymal stem cells. Recently, we have developed a novel system of quantitative PCR for detection of BDNF mRNA isoforms. By using this system, we identified the exon-specific mode of expression in acute and chronic pain. In addition, we have made gene expression profiles of KO mice of beta2 subunits in acetylcholine receptors.

  5. The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification

    Science.gov (United States)

    Reddy, T.B.K.; Thomas, Alex D.; Stamatis, Dimitri; Bertsch, Jon; Isbandi, Michelle; Jansson, Jakob; Mallajosyula, Jyothi; Pagani, Ioanna; Lobos, Elizabeth A.; Kyrpides, Nikos C.

    2015-01-01

    The Genomes OnLine Database (GOLD; http://www.genomesonline.org) is a comprehensive online resource to catalog and monitor genetic studies worldwide. GOLD provides up-to-date status on complete and ongoing sequencing projects along with a broad array of curated metadata. Here we report version 5 (v.5) of the database. The newly designed database schema and web user interface supports several new features including the implementation of a four level (meta)genome project classification system and a simplified intuitive web interface to access reports and launch search tools. The database currently hosts information for about 19 200 studies, 56 000 Biosamples, 56 000 sequencing projects and 39 400 analysis projects. More than just a catalog of worldwide genome projects, GOLD is a manually curated, quality-controlled metadata warehouse. The problems encountered in integrating disparate and varying quality data into GOLD are briefly highlighted. GOLD fully supports and follows the Genomic Standards Consortium (GSC) Minimum Information standards. PMID:25348402

  6. The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, Tatiparthi B. K. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Thomas, Alex D. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Stamatis, Dimitri [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Bertsch, Jon [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Isbandi, Michelle [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Jansson, Jakob [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Mallajosyula, Jyothi [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Pagani, Ioanna [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Lobos, Elizabeth A. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Kyrpides, Nikos C. [USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)

    2014-10-27

    The Genomes OnLine Database (GOLD; http://www.genomesonline.org) is a comprehensive online resource to catalog and monitor genetic studies worldwide. GOLD provides up-to-date status on complete and ongoing sequencing projects along with a broad array of curated metadata. Within this paper, we report version 5 (v.5) of the database. The newly designed database schema and web user interface supports several new features including the implementation of a four level (meta)genome project classification system and a simplified intuitive web interface to access reports and launch search tools. The database currently hosts information for about 19 200 studies, 56 000 Biosamples, 56 000 sequencing projects and 39 400 analysis projects. More than just a catalog of worldwide genome projects, GOLD is a manually curated, quality-controlled metadata warehouse. The problems encountered in integrating disparate and varying quality data into GOLD are briefly highlighted. Lastly, GOLD fully supports and follows the Genomic Standards Consortium (GSC) Minimum Information standards.

  7. Genome3D: a UK collaborative project to annotate genomic sequences with predicted 3D structures based on SCOP and CATH domains.

    Science.gov (United States)

    Lewis, Tony E; Sillitoe, Ian; Andreeva, Antonina; Blundell, Tom L; Buchan, Daniel W A; Chothia, Cyrus; Cuff, Alison; Dana, Jose M; Filippis, Ioannis; Gough, Julian; Hunter, Sarah; Jones, David T; Kelley, Lawrence A; Kleywegt, Gerard J; Minneci, Federico; Mitchell, Alex; Murzin, Alexey G; Ochoa-Montaño, Bernardo; Rackham, Owen J L; Smith, James; Sternberg, Michael J E; Velankar, Sameer; Yeats, Corin; Orengo, Christine

    2013-01-01

    Genome3D, available at http://www.genome3d.eu, is a new collaborative project that integrates UK-based structural resources to provide a unique perspective on sequence-structure-function relationships. Leading structure prediction resources (DomSerf, FUGUE, Gene3D, pDomTHREADER, Phyre and SUPERFAMILY) provide annotations for UniProt sequences to indicate the locations of structural domains (structural annotations) and their 3D structures (structural models). Structural annotations and 3D model predictions are currently available for three model genomes (Homo sapiens, E. coli and baker's yeast), and the project will extend to other genomes in the near future. As these resources exploit different strategies for predicting structures, the main aim of Genome3D is to enable comparisons between all the resources so that biologists can see where predictions agree and are therefore more trusted. Furthermore, as these methods differ in whether they build their predictions using CATH or SCOP, Genome3D also contains the first official mapping between these two databases. This has identified pairs of similar superfamilies from the two resources at various degrees of consensus (532 bronze pairs, 527 silver pairs and 370 gold pairs).

  8. Human genome libraries. Final progress report, February 1, 1994--August 31, 1997

    Energy Technology Data Exchange (ETDEWEB)

    Kao, Fa-Ten

    1998-01-01

    The goal of this program is to use a novel technology of chromosome microdissection and microcloning to construct chromosome region-specific libraries as resources for various human genome program studies. Region specific libraries have been constructed for the entire human chromosomes 2 and 18.

  9. 78 FR 47674 - Genome in a Bottle Consortium-Progress and Planning Workshop

    Science.gov (United States)

    2013-08-06

    ... quantitative performance metrics for confidence in variant calling. These standards and quantitative..., reproducible research and regulated applications in the clinic. On April 13, 2012, NIST convened the workshop... Material (RM) Selection and Design: select appropriate sources for whole genome RMs and identify or design...

  10. Genomes

    National Research Council Canada - National Science Library

    Brown, T. A. (Terence A.)

    2002-01-01

    ... of genome expression and replication processes, and transcriptomics and proteomics. This text is richly illustrated with clear, easy-to-follow, full color diagrams, which are downloadable from the book's website...

  11. Consortium biology in immunology: the perspective from the Immunological Genome Project.

    Science.gov (United States)

    Benoist, Christophe; Lanier, Lewis; Merad, Miriam; Mathis, Diane

    2012-10-01

    Although the field has a long collaborative tradition, immunology has made less use than genetics of 'consortium biology', wherein groups of investigators together tackle large integrated questions or problems. However, immunology is naturally suited to large-scale integrative and systems-level approaches, owing to the multicellular and adaptive nature of the cells it encompasses. Here, we discuss the value and drawbacks of this organization of research, in the context of the long-running 'big science' debate, and consider the opportunities that may exist for the immunology community. We position this analysis in light of our own experience, both positive and negative, as participants of the Immunological Genome Project.

  12. ELSI Bibliography: Ethical legal and social implications of the Human Genome Project

    Energy Technology Data Exchange (ETDEWEB)

    Yesley, M.S. [comp.

    1993-11-01

    This second edition of the ELSI Bibliography provides a current and comprehensive resource for identifying publications on the major topics related to the ethical, legal and social issues (ELSI) of the Human Genome Project. Since the first edition of the ELSI Bibliography was printed last year, new publications and earlier ones identified by additional searching have doubled our computer database of ELSI publications to over 5600 entries. The second edition of the ELSI Bibliography reflects this growth of the underlying computer database. Researchers should note that an extensive collection of publications in the database is available for public use at the General Law Library of Los Alamos National Laboratory (LANL).

  13. Genomic Instability Promoted by Overexpression of Mismatch Repair Factors in Yeast: A Model for Understanding Cancer Progression.

    Science.gov (United States)

    Chakraborty, Ujani; Dinh, Timothy A; Alani, Eric

    2018-04-13

    Mismatch repair (MMR) proteins act in spellchecker roles to excise misincorporation errors that occur during DNA replication. Curiously, large-scale analyses of a variety of cancers showed that increased expression of MMR proteins often correlated with tumor aggressiveness, metastasis, and early recurrence. To better understand these observations, we used the TCGA and GENT databases to analyze MMR protein expression in cancers. We found that the MMR genes MSH2 and MSH6 are overexpressed more frequently than MSH3 , and that MSH2 and MSH6 are often co-overexpressed as a result of copy number amplifications of these genes. These observations encouraged us to test the effects of upregulating MMR protein levels in baker's yeast, where we can sensitively monitor genome instability phenotypes associated with cancer initiation and progression. Msh6 overexpression (2 to 4-fold) almost completely disrupted mechanisms that prevent recombination between divergent DNA sequences by interacting with the DNA polymerase processivity clamp PCNA and by sequestering the Sgs1 helicase. Importantly, co-overexpression of Msh2 and Msh6 (∼8-fold) conferred, in a PCNA interaction dependent manner, several genome instability phenotypes including increased mutation rate, increased sensitivity to the DNA replication inhibitor hydroxyurea and the DNA damaging agents methyl methanesulfonate and 4-nitroquinoline N-oxide, and elevated loss of heterozygosity. Msh2 and Msh6 co-overexpression also altered the cell cycle distribution of exponentially growing cells, resulting in an increased fraction of unbudded cells, consistent with a larger percentage of cells in G1. These novel observations suggested that overexpression of MSH factors affected the integrity of the DNA replication fork, causing genome instability phenotypes that could be important for promoting cancer progression. Copyright © 2018, Genetics.

  14. Importing statistical measures into Artemis enhances gene identification in the Leishmania genome project

    Directory of Open Access Journals (Sweden)

    McDonagh Paul D

    2003-06-01

    Full Text Available Abstract Background Seattle Biomedical Research Institute (SBRI as part of the Leishmania Genome Network (LGN is sequencing chromosomes of the trypanosomatid protozoan species Leishmania major. At SBRI, chromosomal sequence is annotated using a combination of trained and untrained non-consensus gene-prediction algorithms with ARTEMIS, an annotation platform with rich and user-friendly interfaces. Results Here we describe a methodology used to import results from three different protein-coding gene-prediction algorithms (GLIMMER, TESTCODE and GENESCAN into the ARTEMIS sequence viewer and annotation tool. Comparison of these methods, along with the CODONUSAGE algorithm built into ARTEMIS, shows the importance of combining methods to more accurately annotate the L. major genomic sequence. Conclusion An improvised and powerful tool for gene prediction has been developed by importing data from widely-used algorithms into an existing annotation platform. This approach is especially fruitful in the Leishmania genome project where there is large proportion of novel genes requiring manual annotation.

  15. [Progress of genome engineering technology via clustered regularly interspaced short palindromic repeats--a review].

    Science.gov (United States)

    Li, Hao; Qiu, Shaofu; Song, Hongbin

    2013-10-04

    In survival competition with phage, bacteria and archaea gradually evolved the acquired immune system--Clustered regularly interspaced short palindromic repeats (CRISPR), presenting the trait of transcribing the crRNA and the CRISPR-associated protein (Cas) to silence or cleaving the foreign double-stranded DNA specifically. In recent years, strong interest arises in prokaryotes primitive immune system and many in-depth researches are going on. Recently, researchers successfully repurposed CRISPR as an RNA-guided platform for sequence-specific gene expression, which provides a simple approach for selectively perturbing gene expression on a genome-wide scale. It will undoubtedly bring genome engineering into a more convenient and accurate new era.

  16. Human Genome Project discoveries: Dialectics and rhetoric in the science of genetics

    Science.gov (United States)

    Robidoux, Charlotte A.

    The Human Genome Project (HGP), a $437 million effort that began in 1990 to chart the chemical sequence of our three billion base pairs of DNA, was completed in 2003, marking the 50th anniversary that proved the definitive structure of the molecule. This study considered how dialectical and rhetorical arguments functioned in the science, political, and public forums over a 20-year period, from 1980 to 2000, to advance human genome research and to establish the official project. I argue that Aristotle's continuum of knowledge--which ranges from the probable on one end to certified or demonstrated knowledge on the other--provides useful distinctions for analyzing scientific reasoning. While contemporary scientific research seeks to discover certified knowledge, investigators generally employ the hypothetico-deductive or scientific method, which often yields probable rather than certain findings, making these dialectical in nature. Analysis of the discourse describing human genome research revealed the use of numerous rhetorical figures and topics. Persuasive and probable reasoning were necessary for scientists to characterize unknown genetic phenomena, to secure interest in and funding for large-scale human genome research, to solve scientific problems, to issue probable findings, to convince colleagues and government officials that the findings were sound and to disseminate information to the public. Both government and private venture scientists drew on these tools of reasoning to promote their methods of mapping and sequencing the genome. The debate over how to carry out sequencing was rooted in conflicting values. Scientists representing the academic tradition valued a more conservative method that would establish high quality results, and those supporting private industry valued an unconventional approach that would yield products and profits more quickly. Values in turn influenced political and public forum arguments. Agency representatives and investors sided

  17. Salt repository project: Technical progress report for the quarter 1 April--30 June 1987

    International Nuclear Information System (INIS)

    1987-01-01

    This document reports the progress being made each quarter on the development of a geologic repository in salt for the permanent disposal of high-level nuclear waste. The reporting elements are arranged by the work breakdown structure so that related studies are presented together. The studies are reported by the Office of Nuclear Waste Isolation, a prime contractor of the US Department of Energy (DOE) Salt Repository Project Office. The studies include work by other DOE prime contractors and by contractors to the Office of Nuclear Waste Isolation

  18. Salt Repository Project technical progress report for the quarter 1 January--31 March 1987

    International Nuclear Information System (INIS)

    1988-03-01

    This document reports the progress being made each quarter on the development of a geologic repository in salt for the permanent disposal of high-level nuclear waste. The reporting elements are arranged by the work breakdown structure so that related studies are presented together. The studies are reported by the Office of Nuclear Waste Isolation, a prime contractor of the US Department of energy (DOE) Salt Repository Project Office. The studies include work by other DOE prime contractors and by contractors to the Office of Nuclear Waste Isolation. 23 refs., 1 fig

  19. Commentary: The Materials Project: A materials genome approach to accelerating materials innovation

    Directory of Open Access Journals (Sweden)

    Anubhav Jain

    2013-07-01

    Full Text Available Accelerating the discovery of advanced materials is essential for human welfare and sustainable, clean energy. In this paper, we introduce the Materials Project (www.materialsproject.org, a core program of the Materials Genome Initiative that uses high-throughput computing to uncover the properties of all known inorganic materials. This open dataset can be accessed through multiple channels for both interactive exploration and data mining. The Materials Project also seeks to create open-source platforms for developing robust, sophisticated materials analyses. Future efforts will enable users to perform ‘‘rapid-prototyping’’ of new materials in silico, and provide researchers with new avenues for cost-effective, data-driven materials design.

  20. A haplotype regression approach for genetic evaluation using sequences from the 1000 bull genomes Project

    International Nuclear Information System (INIS)

    Lakhssassi, K.; González-Recio, O.

    2017-01-01

    Haplotypes from sequencing data may improve the prediction accuracy in genomic evaluations as haplotypes are in stronger linkage disequilibrium with quantitative trait loci than markers from SNP chips. This study focuses first, on the creation of haplotypes in a population sample of 450 Holstein animals, with full-sequence data from the 1000 bull genomes project; and second, on incorporating them into the whole genome prediction model. In total, 38,319,258 SNPs (and indels) from Next Generation Sequencing were included in the analysis. After filtering variants with minor allele frequency (MAF< 0.025) 13,912,326 SNPs were available for the haplotypes extraction with findhap.f90. The number of SNPs in the haploblocks was on average 924 SNP (166,552 bp). Unique haplotypes were around 97% in all chromosomes and were ignored leaving 153,428 haplotypes. Estimated haplotypes had a large contribution to the total variance of genomic estimated breeding values for kilogram of protein, Global Type Index, Somatic Cell Score and Days Open (between 32 and 99.9%). Haploblocks containing haplotypes with large effects were selected by filtering for each trait, haplotypes whose effect was larger/lower than the mean plus/minus 3 times the standard deviation (SD) and 1 SD above the mean of the haplotypes effect distribution. Results showed that filtering by 3 SD would not be enough to capture a large proportion of genetic variance, whereas filtering by 1 SD could be useful but model convergence should be considered. Additionally, sequence haplotypes were able to capture additional genetic variance to the polygenic effect for traits undergoing lower selection intensity like fertility and health traits.

  1. A haplotype regression approach for genetic evaluation using sequences from the 1000 bull genomes Project

    Energy Technology Data Exchange (ETDEWEB)

    Lakhssassi, K.; González-Recio, O.

    2017-07-01

    Haplotypes from sequencing data may improve the prediction accuracy in genomic evaluations as haplotypes are in stronger linkage disequilibrium with quantitative trait loci than markers from SNP chips. This study focuses first, on the creation of haplotypes in a population sample of 450 Holstein animals, with full-sequence data from the 1000 bull genomes project; and second, on incorporating them into the whole genome prediction model. In total, 38,319,258 SNPs (and indels) from Next Generation Sequencing were included in the analysis. After filtering variants with minor allele frequency (MAF< 0.025) 13,912,326 SNPs were available for the haplotypes extraction with findhap.f90. The number of SNPs in the haploblocks was on average 924 SNP (166,552 bp). Unique haplotypes were around 97% in all chromosomes and were ignored leaving 153,428 haplotypes. Estimated haplotypes had a large contribution to the total variance of genomic estimated breeding values for kilogram of protein, Global Type Index, Somatic Cell Score and Days Open (between 32 and 99.9%). Haploblocks containing haplotypes with large effects were selected by filtering for each trait, haplotypes whose effect was larger/lower than the mean plus/minus 3 times the standard deviation (SD) and 1 SD above the mean of the haplotypes effect distribution. Results showed that filtering by 3 SD would not be enough to capture a large proportion of genetic variance, whereas filtering by 1 SD could be useful but model convergence should be considered. Additionally, sequence haplotypes were able to capture additional genetic variance to the polygenic effect for traits undergoing lower selection intensity like fertility and health traits.

  2. The analysis of APOL1 genetic variation and haplotype diversity provided by 1000 Genomes project.

    Science.gov (United States)

    Peng, Ting; Wang, Li; Li, Guisen

    2017-08-11

    The APOL1 gene variants has been shown to be associated with an increased risk of multiple kinds of diseases, particularly in African Americans, but not in Caucasians and Asians. In this study, we explored the single nucleotide polymorphism (SNP) and haplotype diversity of APOL1 gene in different races provided by 1000 Genomes project. Variants of APOL1 gene in 1000 Genome Project were obtained and SNPs located in the regulatory region or coding region were selected for genetic variation analysis. Total 2504 individuals from 26 populations were classified as four groups that included Africa, Europe, Asia and Admixed populations. Tag SNPs were selected to evaluate the haplotype diversities in the four populations by HaploStats software. APOL1 gene was surrounded by some of the most polymorphic genes in the human genome, variation of APOL1 gene was common, with up to 613 SNP (1000 Genome Project reported) and 99 of them (16.2%) with MAF ≥ 1%. There were 79 SNPs in the URR and 92 SNPs in 3'UTR. Total 12 SNPs in URR and 24 SNPs in 3'UTR were considered as common variants with MAF ≥ 1%. It is worth noting that URR-1 was presents lower frequencies in European populations, while other three haplotypes taken an opposite pattern; 3'UTR presents several high-frequency variation sites in a short segment, and the differences of its haplotypes among different population were significant (P < 0.01), UTR-1 and UTR-5 presented much higher frequency in African population, while UTR-2, UTR-3 and UTR-4 were much lower. APOL1 coding region showed that two SNP of G1 with higher frequency are actually pull down the haplotype H-1 frequency when considering all populations pooled together, and the diversity among the four populations be widen by the G1 two mutation (P 1  = 3.33E-4 vs P 2  = 3.61E-30). The distributions of APOL1 gene variants and haplotypes were significantly different among the different populations, in either regulatory or coding regions. It could provide

  3. Analyzing compound and project progress through multi-objective-based compound quality assessment.

    Science.gov (United States)

    Nissink, J Willem M; Degorce, Sébastien

    2013-05-01

    Compound-quality scoring methods designed to evaluate multiple drug properties concurrently are useful to analyze and prioritize output from drug-design efforts. However, formalized multiparameter optimization approaches are not widely used in drug design. We rank molecules synthesized in drug-discovery projects using simple and aggregated desirability functions reflecting medicinal chemistry 'rules'. Our quality score deals transparently with missing data, a key requirement in drug-hunting projects where data availability is often limited. We further estimate confidence in the interpretation of such a compound-quality measure. Scores and associated confidences provide systematic insight in the quality of emerging chemical equity. Tracking quality of synthetic output over time yields valuable insight into the progress of drug-design teams, with potential applications in risk and resource management of a drug portfolio.

  4. Mapping Project on Energy and the Social Sciences. Progress report, October 1, 1978-June 30, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Walker, C.A.; Doob, L.W.; Gould, L.C.

    1979-01-01

    This is a progress report of activities in the fourth year of the Yale Institution for Social and Policy Studies Mapping Project on Energy and the Social Sciences. The Mapping Project evaluates past and present social and behavioral science energy studies, assesses the potential for social and behavioral science contributions to a resolution of the energy problems in the future, and diffuses social and behavioral science information and perspectives to policymakers and others concerned with US or world energy developments. Activities in FY 1979 included meetings, workshops, collecting bibliographic material, publications, evaluating DOE programs in buildings and transportation, performing a special study of potential social impacts of 4 coal technologies, and developing plans for 10 specific research studies on energy.

  5. [Progress report on a World Bank loan to China for a tuberculosis control project].

    Science.gov (United States)

    Zhao, F; Chi, Y; Wang, K

    1995-02-01

    The progress of the World Bank loaned TB control project implemented from the second quarter of 1991 to the fourth quarter of 1993 was described in this paper. In the past three years, 737 counties of the 12 provinces with the population of 360 million has been covered by the project. Among 95176 new smear positive cases discovered, 93909 patients received free treatment of TB. The treatment coverage is 98.7%, of which 95% were treated under full course supervision. The smear conversion rate at two, three months of new smear positive TB patients are 83.4% and 90.6% respectively. The cohort analysis showed that the cure rate is 89.8%, which has reached the advanced level of the modern national tuberculosis control programme in the world.

  6. Crystalline Repository Project: Technical progress report for the period October 1, 1982--May 28, 1986

    International Nuclear Information System (INIS)

    1988-11-01

    This document reports the progress made on the development of a second geologic repository in crystalline rocks during the duration of the Crystalline Repository Project from its inception in October 1982 to its termination in May 1986. The reporting elements are arranged by the work breakdown structure so that related studies are presented together. The studies are reported by the Office of Waste Technology Development (OWTD), successor to the Office of Crystalline Repository Development. OWTD is a prime contractor of the US Department of Energy (DOE) Repository Technology Program Office, itself the successor to the Crystalline Repository Project Office. The studies include work by other DOE prime contractors and by contractors to the Office of Crystalline Repository Development. 151 refs

  7. The Role Of The Integrated, Thematic Project To Learning Progress Of The Child In The Early Period

    Directory of Open Access Journals (Sweden)

    Aida Cornelia Stoian

    2016-12-01

    Full Text Available In this study, we have proposed to present you the results of an empirical research in order to identify the positive aspects of the integrated, thematic project in learning progress of children in preschool. Using the observation method, we analyzed children's results regarding the objectives in the respect to the objectives in the grid. Children's progress in learning represents the confirmation and affirmation of the role of this integrated, thematic project in supporting the early learning child.

  8. Progress in R and D of coated conductor in M-PACC project

    Energy Technology Data Exchange (ETDEWEB)

    Nakamura, T.; Koizumi, T.; Kimura, K. [SWCC Showa Cable Systems Co., Ltd., Kanagawa (Japan); Kato, T. [Japan Fine Ceramics Center, Aichi (Japan); Kiss, T. [Kyushu University, Fukuoka (Japan); Izumi, T.; Ibi, A.; Nakaoka, K. [Superconductivity Research Laboratory, International Superconductivity Technology, Kanagawa (Japan); and others

    2014-06-15

    The five-year national project in Japan for R and D of coated conductors and applications, named as the Materials and Power Applications of Coated Conductors (M-PACC) project, was finished at the end of FY2013. The project consists of four sub-themes as cable, transformer, SMES and coated conductors. In the theme of coated conductors, the fabrication process had been developed to satisfy the requirements from the applications such as in-field I{sub c} performance, low AC loss in the long tapes etc. Through the project, the remarkable progress was achieved as follows; a high in-field minimum I-c value over 54A/cm-width under 3T at 77K was realized in a 200m long EuBCO tape with artificial pinning centers of BaHfO{sub 3} by the pulsed laser deposition (PLD) technique on the IBAD template. On the other hand, the AC loss reduction was confirmed in the tapes fabricated by both PLD and the metal organic deposition (MOD) techniques by scribing 100 m tapes into 10-filaments. Additionally, the mechanism of the delamination phenomenon was systematically investigated and the strength was improved by eliminating the origins of the weak points in the films. Through the development, all targeted goals were accomplished and the several results were appreciated as a world champion data.

  9. TcruziDB, an Integrated Database, and the WWW Information Server for the Trypanosoma cruzi Genome Project

    Directory of Open Access Journals (Sweden)

    Degrave Wim

    1997-01-01

    Full Text Available Data analysis, presentation and distribution is of utmost importance to a genome project. A public domain software, ACeDB, has been chosen as the common basis for parasite genome databases, and a first release of TcruziDB, the Trypanosoma cruzi genome database, is available by ftp from ftp://iris.dbbm.fiocruz.br/pub/genomedb/TcruziDB as well as versions of the software for different operating systems (ftp://iris.dbbm.fiocruz.br/pub/unixsoft/. Moreover, data originated from the project are available from the WWW server at http://www.dbbm.fiocruz.br. It contains biological and parasitological data on CL Brener, its karyotype, all available T. cruzi sequences from Genbank, data on the EST-sequencing project and on available libraries, a T. cruzi codon table and a listing of activities and participating groups in the genome project, as well as meeting reports. T. cruzi discussion lists (tcruzi-l@iris.dbbm.fiocruz.br and tcgenics@iris.dbbm.fiocruz.br are being maintained for communication and to promote collaboration in the genome project

  10. Dual Roles of RNF2 in Melanoma Progression | Office of Cancer Genomics

    Science.gov (United States)

    Epigenetic regulators have emerged as critical factors governing the biology of cancer. Here, in the context of melanoma, we show that RNF2 is prognostic, exhibiting progression-correlated expression in human melanocytic neoplasms. Through a series of complementary gain-of-function and loss-of-function studies in mouse and human systems, we establish that RNF2 is oncogenic and prometastatic.

  11. Progress update of NASA's free-piston Stirling space power converter technology project

    Science.gov (United States)

    Dudenhoefer, James E.; Winter, Jerry M.; Alger, Donald

    1992-01-01

    A progress update is presented of the NASA LeRC Free-Piston Stirling Space Power Converter Technology Project. This work is being conducted under NASA's Civil Space Technology Initiative (CSTI). The goal of the CSTI High Capacity Power Element is to develop the technology base needed to meet the long duration, high capacity power requirements for future NASA space initiatives. Efforts are focused upon increasing system power output and system thermal and electric energy conversion efficiency at least five fold over current SP-100 technology, and on achieving systems that are compatible with space nuclear reactors. This paper will discuss progress toward 1050 K Stirling Space Power Converters. Fabrication is nearly completed for the 1050 K Component Test Power Converter (CTPC); results of motoring tests of the cold end (525 K), are presented. The success of these and future designs is dependent upon supporting research and technology efforts including heat pipes, bearings, superalloy joining technologies, high efficiency alternators, life and reliability testing, and predictive methodologies. This paper will compare progress in significant areas of component development from the start of the program with the Space Power Development Engine (SPDE) to the present work on CTPC.

  12. Yucca Mountain Project - Argonne National Laboratory annual progress report, FY 1994

    Energy Technology Data Exchange (ETDEWEB)

    Bates, J.K.; Fortner, J.A.; Finn, P.A.; Wronkiewicz, D.J.; Hoh, J.C.; Emery, J.W.; Buck, E.C.; Wolf, S.F.

    1995-02-01

    This document reports on the work done by the Nuclear Waste Management Section of the Chemical Technology Division (CMT), Argonne National Laboratory, in the period October 1993-September 1994. Studies have been performed to evaluate the performance of nuclear waste glass and spent fuel samples under unsaturated conditions (low volume water contact) that are likely to exist in the Yucca Mountain environment being considered as a potential site for a high-level waste repository. Tests with simulated waste glasses have been in progress for over eight years and demonstrate that actinides from initially fresh glass surfaces will be released as a result of the spallation of reacted glass layers from the surface, as the small volume of water passes over the waste form. Studies are also underway to evaluate the performance of spent fuel samples and unirradiated UO{sub 2} in projected repository conditions. Tests with UO{sub 2} have been ongoing for nine years and show that the oxidation of UO{sub 2} occurs rapidly, and the resulting paragenetic sequence of secondary phases that form on the sample surface is similar to that observed in natural analogues. The reaction of spent fuel samples under conditions similar to those used with UO{sub 2} have been in progress for nearly two years, and the results suggest that spent fuel follows the same reaction progress as UO{sub 2}. The release of individual fission products and transuranic elements was not congruent, with the release being controlled by the formation of small particles or colloids that are suspended in solution and transported away from the waste form. The reaction progress depends on the composition of the spent fuel samples used and, likely, on the composition of the groundwater that contacts the waste form.

  13. Low-Cost Solar Array Project. Progress report 12, January-April 1979 and proceedings of the 12th Project Integration Meeting

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    This report describes progress made by the Low-Cost Solar Array Project during the period January through April 1979. It includes reports on project analysis and integration; technology development in silicon material, large-area sheet silicon, and encapsulation; production process and equipment development; engineering and operations, and a discussion of the steps taken to integrate these efforts. It includes a report on, and copies of viewgraphs presented at the Project Integration Meeting held April 4-5, 1979.

  14. Genomic Diversity and the Microenvironment as Drivers of Progression in DCIS

    Science.gov (United States)

    2015-10-01

    progress to invasive breast cancer. We have applied for and received ethical approval to carry out the study at our primary site, and have completed a...cancer, and the application of ecological and other spatial statistics to those images 1-4. 24 Month Milestones: 3 • IHC staining of candidate...Athena Aktipis and Yinyin Yuan: An ecological measure of immune-cancer colocalization as a prognostic factor for breast cancer. Under review at Breast

  15. Genomic analyses of breast cancer progression reveal distinct routes of metastasis emergence

    DEFF Research Database (Denmark)

    Krøigård, Anne Bruun; Larsen, Martin Jakob; Brasch-Andersen, Charlotte

    2017-01-01

    receptor (ER)-positive breast cancer. Our data provide support for both linear and parallel progression towards metastasis. We report for the first time evidence of metastasis-to-metastasis seeding in breast cancer. Our results point to three distinct routes of metastasis emergence. This may have profound...... clinical implications and provides substantial novel molecular insights into the timing and mutational evolution of breast cancer metastasis....

  16. Implementation and assessment of a yeast orphan gene research project: involving undergraduates in authentic research experiences and progressing our understanding of uncharacterized open reading frames.

    Science.gov (United States)

    Bowling, Bethany V; Schultheis, Patrick J; Strome, Erin D

    2016-02-01

    Saccharomyces cerevisiae was the first eukaryotic organism to be sequenced; however, little progress has been made in recent years in furthering our understanding of all open reading frames (ORFs). From October 2012 to May 2015 the number of verified ORFs had only risen from 75.31% to 78%, while the number of uncharacterized ORFs had decreased from 12.8% to 11% (representing > 700 genes still left in this category; http://www.yeastgenome.org/genomesnapshot). Course-based research has been shown to increase student learning while providing experience with real scientific investigation; however, implementation in large, multi-section courses presents many challenges. This study sought to test the feasibility and effectiveness of incorporating authentic research into a core genetics course, with multiple instructors, to increase student learning and progress our understanding of uncharacterized ORFs. We generated a module-based annotation toolkit and utilized easily accessible bioinformatics tools to predict gene function for uncharacterized ORFs within the Saccharomyces Genome Database (SGD). Students were each assigned an uncharacterized ORF, which they annotated using contemporary comparative genomics methodologies, including multiple sequence alignment, conserved domain identification, signal peptide prediction and cellular localization algorithms. Student learning outcomes were measured by quizzes, project reports and presentations, as well as a post-project questionnaire. Our results indicate that the authentic research experience had positive impacts on students' perception of their learning and their confidence to conduct future research. Furthermore, we believe that creation of an online repository and adoption and/or adaptation of this project across multiple researchers and institutions could speed the process of gene function prediction. Copyright © 2015 John Wiley & Sons, Ltd.

  17. STYLE - A European project on structural integrity: Progress of the work after 2 Years

    International Nuclear Information System (INIS)

    Heussner, Stefan; Nicak, Tomas; Keim, Elisabeth

    2012-01-01

    The purpose of this paper is to present the progress of the work on the EURATOM project STYLE (Structural integrity for lifetime management - non-RPV components). The project focuses on the structural integrity assessment of reactor coolant pressure boundary components (RCPB) relevant to ageing and life time management. The 4-years project started in January 2010 and is now in its third year. Within STYLE realistic failure models for some of the key components will be identified. The range of assessment tools considered will include those for assessment of component failure by advanced fracture mechanics analyses validated on small and large scale experiments, quantification of weld residual stresses by numerical analysis and by measurements, stress corrosion crack initiation and growth effects and assessment of RCPB components (excluding the reactor pressure vessel) under dynamic and seismic loading. Based on theoretical and experimental results, performance assessment and further development of simplified engineering assessment methods (EAM) will be carried out considering both deterministic and probabilistic approaches. Integrity assessment case studies and large scale demonstration experiments will be performed on Mock-ups of safety relevant components. These will include a repair weld in an aged butt-welded austenitic pipe, a dissimilar narrow gap TIG weld (following the EPR design) and a cladded ferritic pipe. Moreover, experiments on specimens and feature test pieces will be carried out to support the large scale Mock-up analyses. The end product of the project ('STYLE TOOLS') will comprise best practice guidelines on the use of advanced tools, on improvement and qualification of EAM as a part of European Leak-before-break (LBB) procedures and on life time management of the integrity of RCPB components in European nuclear power plants. The project is interacting with the European Network of Excellence NUGENIA (former NULIFE). (author)

  18. Ceramic Technology Project, semiannual progress report for October 1993 through March 1994

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, D.R.

    1994-09-01

    The Ceramic Technology Project was originally developed by the Department of Energy`s Office of Transportation Systems (OTS) in Conservation and Renewable Energy. Significant accomplishments in fabricating ceramic components for the Department of Energy (DOE), National Aeronautics and Space Administration (NASA), and Department of Defense (DoD) advanced heat engine programs have provided evidence that the operation of ceramic parts in high-temperature engine environments is feasible. An assessment of needs was completed, and a five-year project plan was developed with extensive input from private industry. In July 1990, the original plan was updated through the estimated completion of development in 1993. The original objective of the project was to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. During the course of the Ceramic Technology Project, remarkable progress has been made in the development of reliable structural ceramics. The direction of the Ceramic Technology Project is now shifting toward reducing the cost of ceramics to facilitate commercial introduction of ceramic components for near-term engine applications. In response to extensive input from industry, the plan is to extend the engine types which were previously supported (advanced gas turbine and low-heat-rejection diesel engines) to include near-term (5-10 years) applications in conventional automobile and diesel truck engines. To facilitate the rapid transfer of this technology to U.S. industry, the major portion of the work is being done in the ceramic industry, with technological support from government laboratories, other industrial laboratories, and universities. A systematic approach to reducing the cost of components is envisioned.

  19. Improving livestock for agriculture - technological progress from random transgenesis to precision genome editing heralds a new era.

    Science.gov (United States)

    Laible, Götz; Wei, Jingwei; Wagner, Stefan

    2015-01-01

    Humans have a long history in shaping the genetic makeup of livestock to optimize production and meet growing human demands for food and other animal products. Until recently, this has only been possible through traditional breeding and selection, which is a painstakingly slow process of accumulating incremental gains over a long period. The development of transgenic livestock technology offers a more direct approach with the possibility for making genetic improvements with greater impact and within a single generation. However, initially the technology was hampered by technical difficulties and limitations, which have now largely been overcome by progressive improvements over the past 30 years. Particularly, the advent of genome editing in combination with homologous recombination has added a new level of efficiency and precision that holds much promise for the genetic improvement of livestock using the increasing knowledge of the phenotypic impact of genetic sequence variants. So far not a single line of transgenic livestock has gained approval for commercialization. The step change to genome-edited livestock with precise sequence changes may accelerate the path to market, provided applications of this new technology for agriculture can deliver, in addition to economic incentives for producers, also compelling benefits for animals, consumers, and the environment. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Understanding our genetic inheritance: The US Human Genome Project, The first five years FY 1991--1995

    Energy Technology Data Exchange (ETDEWEB)

    None

    1990-04-01

    The Human Genome Initiative is a worldwide research effort with the goal of analyzing the structure of human DNA and determining the location of the estimated 100,000 human genes. In parallel with this effort, the DNA of a set of model organisms will be studied to provide the comparative information necessary for understanding the functioning of the human genome. The information generated by the human genome project is expected to be the source book for biomedical science in the 21st century and will by of immense benefit to the field of medicine. It will help us to understand and eventually treat many of the more than 4000 genetic diseases that affect mankind, as well as the many multifactorial diseases in which genetic predisposition plays an important role. A centrally coordinated project focused on specific objectives is believed to be the most efficient and least expensive way of obtaining this information. The basic data produced will be collected in electronic databases that will make the information readily accessible on convenient form to all who need it. This report describes the plans for the U.S. human genome project and updates those originally prepared by the Office of Technology Assessment (OTA) and the National Research Council (NRC) in 1988. In the intervening two years, improvements in technology for almost every aspect of genomics research have taken place. As a result, more specific goals can now be set for the project.

  1. O admirável Projeto Genoma Humano The brave New Human Genome Project

    Directory of Open Access Journals (Sweden)

    Marilena V. Corrêa

    2002-12-01

    Full Text Available Este artigo apresenta um panorama das implicações sociais, éticas e legais do Projeto Genoma Humano. Os benefícios desse megaprojeto, traduzidos em promessas de uma revolução terapêutica na medicina, não se realizarão sem conflitos. O processo de inovação tecnológica na genética traz problemas de ordens diversas: por um lado, pesquisas em consórcio, patenteamento de genes e produtos da genômica apontam interesses comerciais e dificuldades de gerenciamento dos resultados dessas pesquisas. Esses problemas colocam desafios em termos de uma possível desigualdade no acesso aos benefícios das pesquisas. Por outro lado, temos a questão da informação genética e da proteção de dados individuais sobre riscos e suscetibilidades a doenças e atributos humanos. O problema da definição de homens e mulheres em função de traços genéticos traz uma ameaça discriminatória clara, e se torna agudo em função do reducionismo genético que a mídia ajuda a propagar. As respostas a esses problemas não podem ser esperadas apenas da bioética. A abordagem bioética deve poder combinar-se a análises políticas da reprodução, da sexualidade, da saúde e da medicina. Um vastíssimo espectro de problemas como estes não pode ser discutido em profundidade em um artigo. Optou-se por mapeá-los no sentido de enfatizar em que medida, na reflexão sobre o projeto genoma, a genômica e a pós-genômica, enfrenta-se o desafio de articular aspectos tão diferenciados.This article presents an overview of the social, ethical, and legal implications of the Human Genome Project. The benefits of this mega-project, expressed as promises of a therapeutic revolution in medicine, will not be achieved without conflict. The process of technological innovation in genetics poses problems of various orders: on the one hand, consortium-based research, gene patenting, and genomic products tend to feature commercial interests and management of the results of such

  2. The GenABEL Project for statistical genomics [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Lennart C. Karssen

    2016-05-01

    Full Text Available Development of free/libre open source software is usually done by a community of people with an interest in the tool. For scientific software, however, this is less often the case. Most scientific software is written by only a few authors, often a student working on a thesis. Once the paper describing the tool has been published, the tool is no longer developed further and is left to its own device. Here we describe the broad, multidisciplinary community we formed around a set of tools for statistical genomics. The GenABEL project for statistical omics actively promotes open interdisciplinary development of statistical methodology and its implementation in efficient and user-friendly software under an open source licence. The software tools developed withing the project collectively make up the GenABEL suite, which currently consists of eleven tools. The open framework of the project actively encourages involvement of the community in all stages, from formulation of methodological ideas to application of software to specific data sets. A web forum is used to channel user questions and discussions, further promoting the use of the GenABEL suite. Developer discussions take place on a dedicated mailing list, and development is further supported by robust development practices including use of public version control, code review and continuous integration. Use of this open science model attracts contributions from users and developers outside the “core team”, facilitating agile statistical omics methodology development and fast dissemination.

  3. Human Genome Diversity Project. Summary of planning workshop 3(B): Ethical and human-rights implications

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-12-31

    The third planning workshop of the Human Genome Diversity Project was held on the campus of the US National Institutes of Health in Bethesda, Maryland, from February 16 through February 18, 1993. The second day of the workshop was devoted to an exploration of the ethical and human-rights implications of the Project. This open meeting centered on three roundtables, involving 12 invited participants, and the resulting discussions among all those present. Attendees and their affiliations are listed in the attached Appendix A. The discussion was guided by a schedule and list of possible issues, distributed to all present and attached as Appendix B. This is a relatively complete, and thus lengthy, summary of the comments at the meeting. The beginning of the summary sets out as conclusions some issues on which there appeared to be widespread agreement, but those conclusions are not intended to serve as a set of detailed recommendations. The meeting organizer is distributing his recommendations in a separate memorandum; recommendations from others who attended the meeting are welcome and will be distributed by the meeting organizer to the participants and to the Project committee.

  4. High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?

    Science.gov (United States)

    Leulliot, Nicolas; Trésaugues, Lionel; Bremang, Michael; Sorel, Isabelle; Ulryck, Nathalie; Graille, Marc; Aboulfath, Ilham; Poupon, Anne; Liger, Dominique; Quevillon-Cheruel, Sophie; Janin, Joël; van Tilbeurgh, Herman

    2005-06-01

    Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.

  5. BIOETHICS METHODS IN THE ETHICAL, LEGAL, AND SOCIAL IMPLICATIONS OF THE HUMAN GENOME PROJECT LITERATURE

    Science.gov (United States)

    Walker, Rebecca; Morrissey, Clair

    2013-01-01

    While bioethics as a field has concerned itself with methodological issues since the early years, there has been no systematic examination of how ethics is incorporated into research on the Ethical, Legal and Social Implications (ELSI) of the Human Genome Project. Yet ELSI research may bear a particular burden of investigating and substantiating its methods given public funding, an explicitly cross-disciplinary approach, and the perceived significance of adequate responsiveness to advances in genomics. We undertook a qualitative content analysis of a sample of ELSI publications appearing between 2003-2008 with the aim of better understanding the methods, aims, and approaches to ethics that ELSI researchers employ. We found that the aims of ethics within ELSI are largely prescriptive and address multiple groups. We also found that the bioethics methods used in the ELSI literature are both diverse between publications and multiple within publications, but are usually not themselves discussed or employed as suggested by bioethics method proponents. Ethics in ELSI is also sometimes undistinguished from related inquiries (such as social, legal, or political investigations). PMID:23796275

  6. The human genome project and novel aspects of cytochrome P450 research

    International Nuclear Information System (INIS)

    Ingelman-Sundberg, Magnus

    2005-01-01

    Currently, 57 active cytochrome P450 (CYP) genes and 58 pseudogenes are known to be present in the human genome. Among the genes discovered by initiatives in the human genome project are CYP2R1, CYP2W1, CYP2S1, CYP2U1 and CYP3A43, the latter apparently encoding a pseudoenzyme. The function, polymorphism and regulation of these genes are still to be discovered to a great extent. The polymorphism of drug metabolizing CYPs is extensive and influences the outcome of drug therapy causing lack of response or adverse drug reactions. The basis for the differences in the global distribution of the polymorphic variants is inactivating gene mutations and subsequent genetic drift. However, polymorphic alleles carrying multiple active gene copies also exist and are suggested in case of CYP2D6 to be caused by positive selection due to development of alkaloid resistance in North East Africa about 10,000-5000 BC. The knowledge about the CYP genes and their polymorphisms is of fundamental importance for effective drug therapy and for drug development as well as for understanding metabolic activation of carcinogens and other xenobiotics. Here, a short review of the current knowledge is given

  7. Mitogenomes from The 1000 Genome Project reveal new Near Eastern features in present-day Tuscans.

    Directory of Open Access Journals (Sweden)

    Alberto Gómez-Carballa

    Full Text Available Genetic analyses have recently been carried out on present-day Tuscans (Central Italy in order to investigate their presumable recent Near East ancestry in connection with the long-standing debate on the origins of the Etruscan civilization. We retrieved mitogenomes and genome-wide SNP data from 110 Tuscans analyzed within the context of The 1000 Genome Project. For phylogeographic and evolutionary analysis we made use of a large worldwide database of entire mitogenomes (>26,000 and partial control region sequences (>180,000.Different analyses reveal the presence of typical Near East haplotypes in Tuscans representing isolated members of various mtDNA phylogenetic branches. As a whole, the Near East component in Tuscan mitogenomes can be estimated at about 8%; a proportion that is comparable to previous estimates but significantly lower than admixture estimates obtained from autosomal SNP data (21%. Phylogeographic and evolutionary inter-population comparisons indicate that the main signal of Near Eastern Tuscan mitogenomes comes from Iran.Mitogenomes of recent Near East origin in present-day Tuscans do not show local or regional variation. This points to a demographic scenario that is compatible with a recent arrival of Near Easterners to this region in Italy with no founder events or bottlenecks.

  8. The development of KMRR schedule and progress control system (KSPCS) for the master schedule of KMRR project

    International Nuclear Information System (INIS)

    Choi, Chang Woong; Lee, Tae Joon; Kim, Joon Yun; Cho, Yun Ho; Hah, Jong Hyun

    1993-07-01

    This report was to development the computerized schedule and progress control system for the master schedule of KMRR project with ARTEMIS 7000/386 CM (Ver. 7.4.2.) based on project management theory (PERT/CPM, PDM, and S-curve). This system has been efficiently used for KMRR master schedule and will be utilized for the detail scheduling of KMRR project. (Author) 23 refs., 26 figs., 52 tabs

  9. Contracts for field projects and supporting research on enhanced oil recovery. Progress review number 83, quarter ending June 30, 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    Summaries of 41 research projects on enhanced recovery are presented under the following sections: (1) chemical flooding; (2) gas displacement; (3) thermal recovery; (4) geoscience technology; (5) resource assessment technology; and (6) reservoir classes. Each presentation gives the title of the project, contract number, research facility, contract date, expected completion data, amount of the award, principal investigator, and DOE program manager, and describes the objectives of the project and a summary of the technical progress.

  10. The development of KMRR schedule and progress control system (KSPCS) for the master schedule of KMRR project

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Chang Woong; Lee, Tae Joon; Kim, Joon Yun; Cho, Yun Ho; Hah, Jong Hyun [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1993-07-01

    This report was to development the computerized schedule and progress control system for the master schedule of KMRR project with ARTEMIS 7000/386 CM (Ver. 7.4.2.) based on project management theory (PERT/CPM, PDM, and S-curve). This system has been efficiently used for KMRR master schedule and will be utilized for the detail scheduling of KMRR project. (Author) 23 refs., 26 figs., 52 tabs.

  11. Progress of and future plans for the L-4 Blanket Project

    International Nuclear Information System (INIS)

    Daenner, W.; Ioki, K.; Cardella, A.

    2001-01-01

    The ITER L-4 Blanket Project has achieved substantial progress over the last two years. The qualification of materials so far considered as reference for the shield module fabrication has been completed, as well as the developments for joining the triplex First Wall structure. Several Primary Wall, baffle, and limiter mock-ups have been manufactured and tested showing comfortable margins against the loads expected in ITER. Shield prototypes have been manufactured by conventional and advanced technology, which have finally demonstrated the manufacturing feasibility. More recently, activities for the qualification of the module attachment system have been started, and first results from materials and mock-up tests have become available. Several test campaigns are still to be finished to complete the data base for the design. In the meantime, further activities have been initiated to adapt the R and D programme to the ITER-FEAT design features, with the aim to further reduce the cost. (author)

  12. Bat Biology, Genomes, and the Bat1K Project: To Generate Chromosome-Level Genomes for All Living Bat Species.

    Science.gov (United States)

    Teeling, Emma C; Vernes, Sonja C; Dávalos, Liliana M; Ray, David A; Gilbert, M Thomas P; Myers, Eugene

    2018-02-15

    Bats are unique among mammals, possessing some of the rarest mammalian adaptations, including true self-powered flight, laryngeal echolocation, exceptional longevity, unique immunity, contracted genomes, and vocal learning. They provide key ecosystem services, pollinating tropical plants, dispersing seeds, and controlling insect pest populations, thus driving healthy ecosystems. They account for more than 20% of all living mammalian diversity, and their crown-group evolutionary history dates back to the Eocene. Despite their great numbers and diversity, many species are threatened and endangered. Here we announce Bat1K, an initiative to sequence the genomes of all living bat species (n∼1,300) to chromosome-level assembly. The Bat1K genome consortium unites bat biologists (>148 members as of writing), computational scientists, conservation organizations, genome technologists, and any interested individuals committed to a better understanding of the genetic and evolutionary mechanisms that underlie the unique adaptations of bats. Our aim is to catalog the unique genetic diversity present in all living bats to better understand the molecular basis of their unique adaptations; uncover their evolutionary history; link genotype with phenotype; and ultimately better understand, promote, and conserve bats. Here we review the unique adaptations of bats and highlight how chromosome-level genome assemblies can uncover the molecular basis of these traits. We present a novel sequencing and assembly strategy and review the striking societal and scientific benefits that will result from the Bat1K initiative.

  13. General-purpose heat source project and space nuclear safety and fuels program. Progress report

    International Nuclear Information System (INIS)

    Maraman, W.J.

    1980-02-01

    Studies related to the use of 238 PuO 2 in radioisotopic power systems carried out for the Advanced Nuclear Systems and Projects Division of LASL are presented. The three programs involved are: general-purpose heat source development; space nuclear safety; and fuels program. Three impact tests were conducted to evaluate the effects of a high temperature reentry pulse and the use of CBCF on impact performance. Additionally, two 238 PuO 2 pellets were encapsulated in Ir-0.3% W for impact testing. Results of the clad development test and vent testing are noted. Results of the environmental tests are summarized. Progress on the Stirling isotope power systems test and the status of the improved MHW tests are indicated. The examination of the impact failure of the iridium shell of MHFT-65 at a fuel pass-through continued. A test plan was written for vibration testing of the assembled light-weight radioisotopic heater unit. Progress on fuel processing is reported

  14. Modelling gas migration in fractured rock. A contribution to the EU's PROGRESS project

    International Nuclear Information System (INIS)

    Humm, J.; Robinson, P.; Clark, K.

    2001-01-01

    To assess the performance of a waste repository, it is necessary to be able to predict the rates of gas generation and to understand and evaluate both the way the gas may disperse from the repository and any effects that might be associated with this dispersal. This document describes the modelling work carried out by QuantiSci on behalf of the UK Environment Agency, in conjunction with the CEC PROGRESS Project (Research into Gas Generation and Migration in Radioactive Waste Repository Systems) which has been carried out as part of the European Commission's IV th framework R and D programme. The project was under the PEGASUS (Projects on the Effects of GAS in Underground Storage facilities) umbrella. A review is provided of alternative conceptual models for the migration of gas through an initially water saturated fracture. A range of front or interface tracking methods for computing gas migration through a fracture is described: direct discretisation, marker particle, volume of fluids and level set methods. Volume of fluids methods are identified as the most appropriate approach for models of this sort. Subsequently, a description is given of the development of a model of gas injection into a single fracture in a portion of Borrowdale Volcanic Granite. The theoretical approach for the model is described in detail and the model compared to experimental results obtained for the real fracture. The experimental results of the CEC PROGRESS Project (obtained using Positron Emission Tomography) do not show particularly good agreement with the model results. However, there are strong indications that this is largely the result of uncertainties in the interpretation of the PET results. The experimental results are acknowledged to be extremely hard to interpret and the apparent negative gas thicknesses observed experimentally confirm this fact. Given the clearly critical dependence of the gas migration pathways on the aperture distribution, any discrepancies of this sort

  15. Human genome. 1993 Program report

    Energy Technology Data Exchange (ETDEWEB)

    1994-03-01

    The purpose of this report is to update the Human Genome 1991-92 Program Report and provide new information on the DOE genome program to researchers, program managers, other government agencies, and the interested public. This FY 1993 supplement includes abstracts of 60 new or renewed projects and listings of 112 continuing and 28 completed projects. These two reports, taken together, present the most complete published view of the DOE Human Genome Program through FY 1993. Research is progressing rapidly toward 15-year goals of mapping and sequencing the DNA of each of the 24 different human chromosomes.

  16. School-Camp Project: progress and prospects; Projeto Campo-Escola: avancos e perspectivas

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira Junior, Jose Baptista de; Pacheco, Almi Cabral; Souza, Thiago Teixeira; Medeiros Junior, Luiz; Santanna, Vanessa Cristina [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Projeto Campo-Escola

    2008-07-01

    The Field-School Project (PCE) was created through an agreement signed in 2003 between the Brazilian Regulatory Petroleum Agency (ANP) and the Federal University of Bahia. The main objectives of the Project were: implementation of a programme of technology transfer to institutions of federal education, and training of specialist for the sector of E and P of oil and gas, from the use of oil fields. Besides that, to demonstrate to small entrepreneurs that it is possible to exploit oil fields at low cost. Nowadays, the PCE has 02 fully equipped camps. The field of Quiambina, with a single well, was the first revitalized field. Equipped with mechanical elevation, it achieved a production of 19,000 bbl of oil until April 2008. The second revitalized field (Fazenda Mamoeiro), also with a single well, is already equipped for the production of gas and oil. Gas will be its primary product. The start of production of this field is planned for July 2008. Apart from these fields, the PCE also has three more: Bela Vista, Caracatu and Riacho Sesmaria. They were also designated by the ANP to integrate the PCE in Bahia. This article shows the progress and prospects for PCE, with emphasis to the field of Fazenda Mamoeiro in its imminent start of production. (author)

  17. Beneficial uses of nuclear waste/sewage sludge irradiation project. Progress report, April 1981-September 1981

    International Nuclear Information System (INIS)

    1982-05-01

    The objective is the further development and transfer of sludge irradiation technology to the municipal sector. At the conclusion of this project, a number of sludge irradiation demonstration plants will have been constructed and placed in operation by municipalities across the country. The following program elements have been established to ensure successful accomplishment of the program objectives: technology transfer by means of municipal demonstration plants; research support and data base development through operation of the SIDSS (Sandia Irradiator for Dried Sewage Solids) pilot plant; engineering support for the demonstration plants by SNL and LANL to include required support for NEPA/licensing activities, risk assessment, safety, and basic design engineering support to A and E firms; life sciences and agricultural R and D support by NMSU for base program activities and for demonstration plants; quality assurance activities to support licensing and other requirements; and program management. During the period covered by this report, the objectives of the BU/SSIP project have been expanded to include determination of an appropriate role for DOE and SNL in food irradiation. Progress on the program elements for the period April 1, 1981 through September 30, 1981, is discussed in detail

  18. S.E.N.S.I.B. project. Progress report 2006; Projet SENSIB. Rapport d'avancement 2006

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    This report presents the state of progress of all the studies which establish at present the S.E.N.S.I.B. project. For year 2006, the progress of the project is globally in compliance with the general schedule of realization of the S.E.N.S.I.B. project and with the perspectives announced in 2005. Factors of sensitivity were identified in diverse circles ( thematic studies) and methods and specific tools of the project are developed. 14 publications (reviews and congress) and 9 I.R.S.N. reports were produced. An international work group was launched to the I.R.S.N. initiative. The web site of S.E.N.S.I.B. on the I.R.S.N. scientific site was built. The S.E.N.S.I.B. project receives a financial participation of the Ademe. (N.C.)

  19. Waste Tank Organic Safety Project organic concentration mechanisms task. FY 1994 progress report

    International Nuclear Information System (INIS)

    Gerber, M.A.

    1994-09-01

    The Pacific Northwest Laboratory (PNL), Waste Tank Organic Safety Project is conducting research to support Westinghouse Hanford Company's (WHC) Waste Tank Safety Program, sponsored by the U.S. Department of Energy's Tank Farm Project Office. The goal of PNL's program is to provide a scientific basis for analyzing organics in Hanford's underground storage tanks (USTs) and for determining whether they are at concentrations that pose a potentially unsafe condition. Part of this research is directed toward determining what organic concentrations are safe by conducting research on organic aging mechanisms and waste energetics to assess the conditions necessary to produce an uncontrolled energy release in tanks due to reactions between the organics and the nitrate and nitrate salts in the tank wastes. The objective of the Organic Concentration Mechanisms Task is to assess the degree of localized enrichment of organics to be expected in the USTs due to concentration mechanisms. This report describes the progress of research conducted in FY 1994 on two concentration mechanisms of interest to the tank safety project: (1) permeation of a separate organic liquid phase into the interstitial spaces of the tank solids during the draining of free liquid from the tanks; and (2) concentration of organics on the surfaces of the solids due to adsorption. Three experiments were conducted to investigate permeation of air and solvent into a sludge simulant that is representative of single-shell tank sludge. The permeation behavior of air and solvent into the sludge simulant can be explained by the properties of the fluid pairs (air/supernate and solvent supernate) and the sludge. One important fluid property is the interfacial tension between the supernate and either the solvent or air. In general, the greater the interfacial tension between two fluids, the more difficult it will be for the air or solvent to displace the supernate during dewatering of the sludge

  20. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project

    DEFF Research Database (Denmark)

    Birney, Ewan; Stamatoyannopoulos, John A; Dutta, Anindya

    2007-01-01

    We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses...

  1. Genome-wide effects of acute progressive feed restriction in liver and white adipose tissue

    International Nuclear Information System (INIS)

    Pohjanvirta, Raimo; Boutros, Paul C.; Moffat, Ivy D.; Linden, Jere; Wendelin, Dominique; Okey, Allan B.

    2008-01-01

    Acute progressive feed restriction (APFR) represents a specific form of caloric restriction in which feed availability is increasingly curtailed over a period of a few days to a few weeks. It is often used for control animals in toxicological and pharmacological studies on compounds causing body weight loss to equalize weight changes between experimental and control groups and thereby, intuitively, to also set their metabolic states to the same phase. However, scientific justification for this procedure is lacking. In the present study, we analyzed by microarrays the impact on hepatic gene expression in rats of two APFR regimens that caused identical diminution of body weight (19%) but differed slightly in duration (4 vs. 10 days). In addition, white adipose tissue (WAT) was also subjected to the transcriptomic analysis on day-4. The data revealed that the two regimens led to distinct patterns of differentially expressed genes in liver, albeit some major pathways of energy metabolism were similarly affected (particularly fatty acid and amino acid catabolism). The reason for the divergence appeared to be entrainment by the longer APFR protocol of peripheral oscillator genes, which resulted in derailment of circadian rhythms and consequent interaction of altered diurnal fluctuations with metabolic adjustments in gene expression activities. WAT proved to be highly unresponsive to the 4-day APFR as only 17 mRNA levels were influenced by the treatment. This study demonstrates that body weight is a poor proxy of metabolic state and that the customary protocols of feed restriction can lead to rhythm entrainment

  2. [Research progresses of the completed pediatrics projects funded by National Natural Science Foundation of China from 2002 to 2006].

    Science.gov (United States)

    Xu, Ling; Hao, Jie; Deng, Min; Xu, Yan-ying

    2009-05-01

    To understand the projects completion and research progresses in pediatrics which were funded by the National Natural Science Foundation of China (NSFC), and evaluate the accomplishment objectively and justly. The completion status of projects in pediatrics funded by department of clinical medicine II from 2002 to 2006 was analysed retrospectively, and important research achievement and outstanding development in some projects were reported. During the period between 2002 and 2006, 420 articles were published, and the average was 8.1 papers per project, which included 56 papers that were published in journals indexed by SCI (the average was 1.1 papers per project). The completion of general project was better than that of "the Young Researchers Fund" and small grant project. Ten post-doctors, 102 doctors and 109 masters were trained. Two projects were awarded with the first grade prize and another 2 with the second grade prize at the provincial and ministerial level, 4 items applied for patent and 1 was granted. These completed projects, which were mainly related to 7 of 12 subspecialties in the field of pediatrics, such as the respiratory disease, nephrology, neurology, cardiology, endocrinology, hematology, neonatology, are the major portion of the application projects and subsidized projects funded by NSFC, and achieved great research progresses. During the period between 2002 and 2006, the 52 completed projects in pediatrics showed difference in the distribution and quality of accomplishment among subspecialties and among types of supported projects; there are some gaps between pediatrics and some other clinical basic subspecialties II, this situation released the research status and problems in development of pediatrics in China. The general projects completion was good, and many projects obtained research achievements, which reflect the leading function of NSFC in pediatric research.

  3. Canine tumor cross-species genomics uncovers targets linked to osteosarcoma progression

    Science.gov (United States)

    2009-01-01

    Background Pulmonary metastasis continues to be the most common cause of death in osteosarcoma. Indeed, the 5-year survival for newly diagnosed osteosarcoma patients has not significantly changed in over 20 years. Further understanding of the mechanisms of metastasis and resistance for this aggressive pediatric cancer is necessary. Pet dogs naturally develop osteosarcoma providing a novel opportunity to model metastasis development and progression. Given the accelerated biology of canine osteosarcoma, we hypothesized that a direct comparison of canine and pediatric osteosarcoma expression profiles may help identify novel metastasis-associated tumor targets that have been missed through the study of the human cancer alone. Results Using parallel oligonucleotide array platforms, shared orthologues between species were identified and normalized. The osteosarcoma expression signatures could not distinguish the canine and human diseases by hierarchical clustering. Cross-species target mining identified two genes, interleukin-8 (IL-8) and solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), which were uniformly expressed in dog but not in all pediatric osteosarcoma patient samples. Expression of these genes in an independent population of pediatric osteosarcoma patients was associated with poor outcome (p = 0.020 and p = 0.026, respectively). Validation of IL-8 and SLC1A3 protein expression in pediatric osteosarcoma tissues further supported the potential value of these novel targets. Ongoing evaluation will validate the biological significance of these targets and their associated pathways. Conclusions Collectively, these data support the strong similarities between human and canine osteosarcoma and underline the opportunities provided by a comparative oncology approach as a means to improve our understanding of cancer biology and therapies. PMID:20028558

  4. Canine tumor cross-species genomics uncovers targets linked to osteosarcoma progression

    Directory of Open Access Journals (Sweden)

    Triche Timothy

    2009-12-01

    Full Text Available Abstract Background Pulmonary metastasis continues to be the most common cause of death in osteosarcoma. Indeed, the 5-year survival for newly diagnosed osteosarcoma patients has not significantly changed in over 20 years. Further understanding of the mechanisms of metastasis and resistance for this aggressive pediatric cancer is necessary. Pet dogs naturally develop osteosarcoma providing a novel opportunity to model metastasis development and progression. Given the accelerated biology of canine osteosarcoma, we hypothesized that a direct comparison of canine and pediatric osteosarcoma expression profiles may help identify novel metastasis-associated tumor targets that have been missed through the study of the human cancer alone. Results Using parallel oligonucleotide array platforms, shared orthologues between species were identified and normalized. The osteosarcoma expression signatures could not distinguish the canine and human diseases by hierarchical clustering. Cross-species target mining identified two genes, interleukin-8 (IL-8 and solute carrier family 1 (glial high affinity glutamate transporter, member 3 (SLC1A3, which were uniformly expressed in dog but not in all pediatric osteosarcoma patient samples. Expression of these genes in an independent population of pediatric osteosarcoma patients was associated with poor outcome (p = 0.020 and p = 0.026, respectively. Validation of IL-8 and SLC1A3 protein expression in pediatric osteosarcoma tissues further supported the potential value of these novel targets. Ongoing evaluation will validate the biological significance of these targets and their associated pathways. Conclusions Collectively, these data support the strong similarities between human and canine osteosarcoma and underline the opportunities provided by a comparative oncology approach as a means to improve our understanding of cancer biology and therapies.

  5. Deep brain stimulation, brain maps and personalized medicine: lessons from the human genome project.

    Science.gov (United States)

    Fins, Joseph J; Shapiro, Zachary E

    2014-01-01

    Although the appellation of personalized medicine is generally attributed to advanced therapeutics in molecular medicine, deep brain stimulation (DBS) can also be so categorized. Like its medical counterpart, DBS is a highly personalized intervention that needs to be tailored to a patient's individual anatomy. And because of this, DBS like more conventional personalized medicine, can be highly specific where the object of care is an N = 1. But that is where the similarities end. Besides their differing medical and surgical provenances, these two varieties of personalized medicine have had strikingly different impacts. The molecular variant, though of a more recent vintage has thrived and is experiencing explosive growth, while DBS still struggles to find a sustainable therapeutic niche. Despite its promise, and success as a vetted treatment for drug resistant Parkinson's Disease, DBS has lagged in broadening its development, often encountering regulatory hurdles and financial barriers necessary to mount an adequate number of quality trials. In this paper we will consider why DBS-or better yet neuromodulation-has encountered these challenges and contrast this experience with the more successful advance of personalized medicine. We will suggest that personalized medicine and DBS's differential performance can be explained as a matter of timing and complexity. We believe that DBS has struggled because it has been a journey of scientific exploration conducted without a map. In contrast to molecular personalized medicine which followed the mapping of the human genome and the Human Genome Project, DBS preceded plans for the mapping of the human brain. We believe that this sequence has given personalized medicine a distinct advantage and that the fullest potential of DBS will be realized both as a cartographical or electrophysiological probe and as a modality of personalized medicine.

  6. Rhipicephalus microplus strain Deutsch, whole genome shotgun sequencing project Version 2

    Science.gov (United States)

    The cattle tick, Rhipicephalus (Boophilus) microplus, has a genome over 2.4 times the size of the human genome, and with over 70% of repetitive DNA, this genome would prove very costly to sequence at today's prices and difficult to assemble and analyze. Cot filtration/selection techniques were used ...

  7. Snake Genome Sequencing: Results and Future Prospects.

    Science.gov (United States)

    Kerkkamp, Harald M I; Kini, R Manjunatha; Pospelov, Alexey S; Vonk, Freek J; Henkel, Christiaan V; Richardson, Michael K

    2016-12-01

    Snake genome sequencing is in its infancy-very much behind the progress made in sequencing the genomes of humans, model organisms and pathogens relevant to biomedical research, and agricultural species. We provide here an overview of some of the snake genome projects in progress, and discuss the biological findings, with special emphasis on toxinology, from the small number of draft snake genomes already published. We discuss the future of snake genomics, pointing out that new sequencing technologies will help overcome the problem of repetitive sequences in assembling snake genomes. Genome sequences are also likely to be valuable in examining the clustering of toxin genes on the chromosomes, in designing recombinant antivenoms and in studying the epigenetic regulation of toxin gene expression.

  8. Snake Genome Sequencing: Results and Future Prospects

    Directory of Open Access Journals (Sweden)

    Harald M. I. Kerkkamp

    2016-12-01

    Full Text Available Snake genome sequencing is in its infancy—very much behind the progress made in sequencing the genomes of humans, model organisms and pathogens relevant to biomedical research, and agricultural species. We provide here an overview of some of the snake genome projects in progress, and discuss the biological findings, with special emphasis on toxinology, from the small number of draft snake genomes already published. We discuss the future of snake genomics, pointing out that new sequencing technologies will help overcome the problem of repetitive sequences in assembling snake genomes. Genome sequences are also likely to be valuable in examining the clustering of toxin genes on the chromosomes, in designing recombinant antivenoms and in studying the epigenetic regulation of toxin gene expression.

  9. Genomics: The Science and Technology Behind the Human Genome Project (by Charles R. Cantor and Cassandra L. Smith)

    Science.gov (United States)

    Serra, Reviewed By Martin J.

    2000-01-01

    Genomics is one of the most rapidly expanding areas of science. This book is an outgrowth of a series of lectures given by one of the former heads (CRC) of the Human Genome Initiative. The book is designed to reach a wide audience, from biologists with little chemical or physical science background through engineers, computer scientists, and physicists with little current exposure to the chemical or biological principles of genetics. The text starts with a basic review of the chemical and biological properties of DNA. However, without either a biochemistry background or a supplemental biochemistry text, this chapter and much of the rest of the text would be difficult to digest. The second chapter is designed to put DNA into the context of the larger chromosomal unit. Specialized chromosomal structures and sequences (centromeres, telomeres) are introduced, leading to a section on chromosome organization and purification. The next 4 chapters cover the physical (hybridization, electrophoresis), chemical (polymerase chain reaction), and biological (genetic) techniques that provide the backbone of genomic analysis. These chapters cover in significant detail the fundamental principles underlying each technique and provide a firm background for the remainder of the text. Chapters 7­9 consider the need and methods for the development of physical maps. Chapter 7 primarily discusses chromosomal localization techniques, including in situ hybridization, FISH, and chromosome paintings. The next two chapters focus on the development of libraries and clones. In particular, Chapter 9 considers the limitations of current mapping and clone production. The current state and future of DNA sequencing is covered in the next three chapters. The first considers the current methods of DNA sequencing - especially gel-based methods of analysis, although other possible approaches (mass spectrometry) are introduced. Much of the chapter addresses the limitations of current methods, including

  10. Contracts for field projects and supporting research on enhanced oil recovery. Progress review number 86, quarter ending March 31, 1996

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    Summaries are presented for 37 enhanced oil recovery contracts being supported by the Department of Energy. The projects are grouped into gas displacement methods, thermal recovery methods, geoscience technology, reservoir characterization, and field demonstrations in high-priority reservoir classes. Each summary includes the objectives of the project and a summary of the technical progress, as well as information on contract dates, size of award, principal investigator, and company or facility doing the research.

  11. RCRA [Resource Conservation and Recovery Act] ground-water monitoring projects for Hanford facilities: Annual progress report for 1988

    International Nuclear Information System (INIS)

    Fruland, R.M.; Lundgren, R.E.

    1989-04-01

    This report describes the progress during 1988 of 14 Hanford Site ground-water monitoring projects covering 16 hazardous waste facilities and 1 nonhazardous waste facility (the Solid Waste Landfill). Each of the projects is being conducted according to federal regulations based on the Resource Conservation and Recovery Act (RCRA) of 1976 and the State of Washington Administrative Code. 21 refs., 23 figs., 8 tabs

  12. Lawrence Livermore National Laboratory- Completing the Human Genome Project and Triggering Nearly $1 Trillion in U.S. Economic Activity

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, Jeffrey S. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-07-28

    The success of the Human Genome project is already nearing $1 Trillion dollars of U.S. economic activity. Lawrence Livermore National Laboratory (LLNL) was a co-leader in one of the biggest biological research effort in history, sequencing the Human Genome Project. This ambitious research effort set out to sequence the approximately 3 billion nucleotides in the human genome, an effort many thought was nearly impossible. Deoxyribonucleic acid (DNA) was discovered in 1869, and by 1943 came the discovery that DNA was a molecule that encodes the genetic instructions used in the development and functioning of living organisms and many viruses. To make full use of the information, scientists needed to first sequence the billions of nucleotides to begin linking them to genetic traits and illnesses, and eventually more effective treatments. New medical discoveries and improved agriculture productivity were some of the expected benefits. While the potential benefits were vast, the timeline (over a decade) and cost ($3.8 Billion) exceeded what the private sector would normally attempt, especially when this would only be the first phase toward the path to new discoveries and market opportunities. The Department of Energy believed its best research laboratories could meet this Grand Challenge and soon convinced the National Institute of Health to formally propose the Human Genome project to the federal government. The U.S. government accepted the risk and challenge to potentially create new healthcare and food discoveries that could benefit the world and the U.S. Industry.

  13. Geothermal direct-heat utilization assistance. Quarterly project progress report, October--December 1997

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-01-01

    This report summarizes geothermal technical assistance, R and D and technology transfer activities of the Geo-Heat Center at Oregon Institute of Technology for the first quarter of FY-98 (October--December 1997). It describes 216 contacts with parties during this period related to technical assistance with geothermal direct heat projects. Areas dealt with include requests for general information including maps and material for high school debates, and material on geothermal heat pumps, resource and well data, space heating and cooling, greenhouses, aquaculture, equipment, district heating, resorts and spas, industrial applications, electric power and snow melting. Research activities include work on model construction specifications of lineshaft submersible pumps and plate heat exchangers, a comprehensive aquaculture developer package and revisions to the Geothermal Direct Use Engineering and Design Guidebook. Outreach activities include the publication of the Quarterly Bulletin (Vol. 18, No. 4) which was devoted entirely to geothermal activities in South Dakota, dissemination of information mainly through mailings of publications, tours of local geothermal uses, geothermal library acquisition and use, participation in workshops, short courses and technical meetings by the staff, and progress monitor reports on geothermal activities.

  14. Federal assistance program. Quarterly project progress report, January 1998--March 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-01

    This report summarizes geothermal technical assistance, R&D and technology transfer activities of the Geo-Heat Center at Oregon Institute of Technology for the second quarter of FY-98-98 (January-March, 1998). It describes 268 contacts with parties during this period related to technical assistance with geothermal direct heat projects. Areas dealt with include requests for general information including maps and material for high school debates, and material on geothermal heat pumps, resource and well data, spacing heating and cooling, greenhouses, aquaculture, equipment, district heating, resorts and spas, industrial applications, electric power and snow melting. Research activities include work on model construction specifications for line shaft submersible pumps and plate heat exchangers and a comprehensive aquaculture developer package. The revised Geothermal Direct Use Engineering and Design Guidebooks was completed, published and is available for distribution. Outreach activities include the publication of the Quarterly Bulletin (Vol. 19, No. 1) which was devoted entirely to geothermal equipment, dissemination of information mainly through mailings of publications, tours of local geothermal uses, geothermal library acquisitions and use, participation in workshops, short courses and technical meetings by the staff, and progress monitor reports on geothermal activities.

  15. 94-1 Research and Development Project lead laboratory support: Fiscal year 1997. Progress report

    International Nuclear Information System (INIS)

    McKee, S.D.

    1996-12-01

    On May 26, 1994, the Defense Nuclear Facilities Safety Board (DNFSB) issued Recommendation 94-1, which expressed the board's concern about nuclear materials left in the manufacturing pipeline after the US halted its nuclear weapons production activities. The DNFSB emphasized the need for remediation of these materials. As part of Recommendation 94-1, the DNFSB defined research objectives as follows: that a research program be established to fill any gaps in the information base needed for choosing among the alternate processes to be used in safe conversion of various types of fissile materials to optimal forms for safe interim storage and the longer-term disposition. To achieve this objective a research and technology development program with two elements is needed: a technology-specific program that is focused on treating and storing materials safety, with concomitant development of storage criteria and surveillance requirements, centered around 3- and 8-year targets; and a core technology program to augment the knowledge base about general chemical and physical processing and storage behavior and to assure safe interim material storage until disposition policies are formulated. The paper reports the progress on the following: materials identification and surveillance; stabilization process development; surveillance and monitoring; core technologies; and project management

  16. St. Louis Area Earthquake Hazards Mapping Project - A Progress Report-November 2008

    Science.gov (United States)

    Karadeniz, D.; Rogers, J.D.; Williams, R.A.; Cramer, C.H.; Bauer, R.A.; Hoffman, D.; Chung, J.; Hempen, G.L.; Steckel, P.H.; Boyd, O.L.; Watkins, C.M.; McCallister, N.S.; Schweig, E.

    2009-01-01

    St. Louis has experienced minor earthquake damage at least 12 times in the past 200 years. Because of this history and its proximity to known active earthquake zones, the St. Louis Area Earthquake Hazards Mapping Project (SLAEHMP) is producing digital maps that show variability of earthquake hazards, including liquefaction and ground shaking, in the St. Louis area. The maps will be available free via the internet. Although not site specific enough to indicate the hazard at a house-by-house resolution, they can be customized by the user to show specific areas of interest, such as neighborhoods or transportation routes. Earthquakes currently cannot be predicted, but scientists can estimate how strongly the ground is likely to shake as the result of an earthquake. Earthquake hazard maps provide one way of conveying such estimates. The U.S. Geological Survey (USGS), which produces earthquake hazard maps for the Nation, is working with local partners to develop detailed maps for urban areas vulnerable to strong ground shaking. These partners, which along with the USGS comprise the SLAEHMP, include the Missouri University of Science and Technology-Rolla (Missouri S&T), Missouri Department of Natural Resources (MDNR), Illinois State Geological Survey (ISGS), Saint Louis University, Missouri State Emergency Management Agency, and URS Corporation. Preliminary hazard maps covering a test portion of the 29-quadrangle St. Louis study area have been produced and are currently being evaluated by the SLAEHMP. A USGS Fact Sheet summarizing this project was produced and almost 1000 copies have been distributed at several public outreach meetings and field trips that have featured the SLAEHMP (Williams and others, 2007). In addition, a USGS website focusing on the SLAEHMP, which provides links to project results and relevant earthquake hazard information, can be found at: http://earthquake.usgs.gov/regional/ceus/urban_map/st_louis/index.php. This progress report summarizes the

  17. Structural analysis of a set of proteins resulting from a bacterial genomics project.

    Science.gov (United States)

    Badger, J; Sauder, J M; Adams, J M; Antonysamy, S; Bain, K; Bergseid, M G; Buchanan, S G; Buchanan, M D; Batiyenko, Y; Christopher, J A; Emtage, S; Eroshkina, A; Feil, I; Furlong, E B; Gajiwala, K S; Gao, X; He, D; Hendle, J; Huber, A; Hoda, K; Kearins, P; Kissinger, C; Laubert, B; Lewis, H A; Lin, J; Loomis, K; Lorimer, D; Louie, G; Maletic, M; Marsh, C D; Miller, I; Molinari, J; Muller-Dieckmann, H J; Newman, J M; Noland, B W; Pagarigan, B; Park, F; Peat, T S; Post, K W; Radojicic, S; Ramos, A; Romero, R; Rutter, M E; Sanderson, W E; Schwinn, K D; Tresser, J; Winhoven, J; Wright, T A; Wu, L; Xu, J; Harris, T J R

    2005-09-01

    The targets of the Structural GenomiX (SGX) bacterial genomics project were proteins conserved in multiple prokaryotic organisms with no obvious sequence homolog in the Protein Data Bank of known structures. The outcome of this work was 80 structures, covering 60 unique sequences and 49 different genes. Experimental phase determination from proteins incorporating Se-Met was carried out for 45 structures with most of the remainder solved by molecular replacement using members of the experimentally phased set as search models. An automated tool was developed to deposit these structures in the Protein Data Bank, along with the associated X-ray diffraction data (including refined experimental phases) and experimentally confirmed sequences. BLAST comparisons of the SGX structures with structures that had appeared in the Protein Data Bank over the intervening 3.5 years since the SGX target list had been compiled identified homologs for 49 of the 60 unique sequences represented by the SGX structures. This result indicates that, for bacterial structures that are relatively easy to express, purify, and crystallize, the structural coverage of gene space is proceeding rapidly. More distant sequence-structure relationships between the SGX and PDB structures were investigated using PDB-BLAST and Combinatorial Extension (CE). Only one structure, SufD, has a truly unique topology compared to all folds in the PDB. Copyright 2005 Wiley-Liss, Inc.

  18. Brain Genomics Superstruct Project initial data release with structural, functional, and behavioral measures.

    Science.gov (United States)

    Holmes, Avram J; Hollinshead, Marisa O; O'Keefe, Timothy M; Petrov, Victor I; Fariello, Gabriele R; Wald, Lawrence L; Fischl, Bruce; Rosen, Bruce R; Mair, Ross W; Roffman, Joshua L; Smoller, Jordan W; Buckner, Randy L

    2015-01-01

    The goal of the Brain Genomics Superstruct Project (GSP) is to enable large-scale exploration of the links between brain function, behavior, and ultimately genetic variation. To provide the broader scientific community data to probe these associations, a repository of structural and functional magnetic resonance imaging (MRI) scans linked to genetic information was constructed from a sample of healthy individuals. The initial release, detailed in the present manuscript, encompasses quality screened cross-sectional data from 1,570 participants ages 18 to 35 years who were scanned with MRI and completed demographic and health questionnaires. Personality and cognitive measures were obtained on a subset of participants. Each dataset contains a T1-weighted structural MRI scan and either one (n=1,570) or two (n=1,139) resting state functional MRI scans. Test-retest reliability datasets are included from 69 participants scanned within six months of their initial visit. For the majority of participants self-report behavioral and cognitive measures are included (n=926 and n=892 respectively). Analyses of data quality, structure, function, personality, and cognition are presented to demonstrate the dataset's utility.

  19. The post-Human Genome Project mindset: race, reliability, and health care.

    Science.gov (United States)

    Kimmelman, J

    2006-11-01

    The following essay reports on the first session of a 2-day workshop on genetic diversity and science communication, organized by the Institute of Genetics. I argue that the four talks in this session reflected two different facets of a 'post-Human Genome Project (HGP)' view of human genetics. The first is characterized by an increasing interest in genetic differences. Two speakers - Troy Duster and Jasber Singh - expressed skepticism about one aspect of this trend: an emphasis on race in medicine and genetics. The other two speakers - Kenneth Weiss and Gustavo Turecki - spoke to a second facet of the post-HGP view: a recognition of the difficulty in translating genetic discovery into medical or public health applications. Though both sets of talks were highly critical of current trends in genetic research, they pulled in opposite directions: one warned about the role of genetics in stabilizing racial categories, while the other lamented the failure of any genetic claims or categories to stabilize at all. I argue that the use of racial categories in medicine seems likely to encounter scientific, medical, and social challenges.

  20. ELSI Bibliography: Ethical, legal and social implications of the Human Genome Project. 1994 Supplement

    Energy Technology Data Exchange (ETDEWEB)

    Yesley, M.S.; Ossorio, P.N. [comps.

    1994-09-01

    This report updates and expands the second edition of the ELSI Bibliography, published in 1993. The Bibliography and Supplement provides a comprehensive resource for identifying publications on the major topics related to the ethical, legal and social issues (ELSI) of the Human Genome Project. The Bibliography and Supplement are extracted from a database compiled at Los Alamos National Laboratory with the support of the Office of Energy Research, US Department of Energy. The second edition of the ELSI Bibliography was dated May 1993 but included publications added to the database until fall 1993. This Supplement reflects approximately 1,000 entries added to the database during the past year, bringing the total to approximately 7,000 entries. More than half of the new entries were published in the last year, and the remainder are earlier publications not previously included in the database. Most of the new entries were published in the academic and professional literature. The remainder are press reports from newspapers of record and scientific journals. The topical listing of the second edition has been followed in the Supplement, with a few changes. The topics of Cystic Fibrosis, Huntington`s Disease, and Sickle Cell Anemia have been combined in a single topic, Disorders. Also, all the entries published in the past year are included in a new topic, Publications: September 1993--September 1994, which provides a comprehensive view of recent reporting and commentary on the science and ELSI of genetics.

  1. Aspergillus oryzae in solid-state and submerged fermentations: Progress report on a multi-disciplinary project

    NARCIS (Netherlands)

    Biesebeke, R. te; Ruijter, G.; Rahardjo, Y.S.P.; Hoogschagen, M.J.; Heerikhuisen, M.; Levin, A.; Driel, K.G.A. van; Schutyser, M.A.I.; Dijksterhuis, J.; Zhu, Y.; Weber, F.J.; Vos, W.M. de; Hondel, K.A.M.J.J. van den; Rinzema, A.; Punt, P.J.

    2002-01-01

    We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated

  2. Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

    NARCIS (Netherlands)

    te Biesebeke, Rob; Ruijter, George; Rahardjo, Yovita S P; Hoogschagen, Marisca J; Heerikhuisen, Margreet; Levin, Ana; van Driel, Kenneth G A; Schutyser, Maarten A I; Dijksterhuis, Jan; Zhu, Yang; Weber, Frans J; de Vos, Willem M; van den Hondel, Kees A M J J; Rinzema, Arjen; Punt, Peter J

    We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated

  3. Aspergillus oryzae in solid-state and submerged fermentations. Progress report on a multi-disciplinary project

    NARCIS (Netherlands)

    Biesebeke, te R.; Ruijter, G.; Rahardjo, Y.S.P.; Hoogschagen, M.J.; Heerikhuisen, M.; Levin, A.; Driel, van K.G.A.; Schutyser, M.A.I.; Dijksterhuis, J.; Yang Zhu, Yang; Weber, F.J.; Vos, de W.M.; Hondel, van den K.A.; Rinzema, A.; Punt, P.J.

    2002-01-01

    We report the progress of a multi-disciplinary research project on solid-state fermentation (SSF) of the filamentous fungus Aspergillus oryzae. The molecular and physiological aspects of the fungus in submerged fermentation (SmF) and SSF are compared and we observe a number of differences correlated

  4. Proyecto genoma humano: un arma de doble filo The Human Genome Project: A double edge weapon

    Directory of Open Access Journals (Sweden)

    Elizabeth Hernández Moore

    2001-04-01

    Full Text Available Después de breve reseña histórica que informa sobre los sorprendentes avances de la genética a partir del descubrimiento de la estructura helicoidal del DNA, el artículo centra su atención en el nacimiento de los estudios genómicos en los Estados Unidos de Norteamérica, las causas y condiciones que los motivaron, hasta desembocar en el multinacional Proyecto Genoma Humano. Sin olvidar la estatura científica de tal empresa, se intenta una mirada desde la perspectiva de las relaciones Norte-Sur, remitiéndonos de modo más incisivo a los aspectos éticos más controvertidos del PGH. Argumentamos que en las sociedades del Sur debemos ocuparnos en jerarquizar los principales problemas bioéticos que nos aquejan y que están aún muy distantes de los que se "encargan" al PGH . Referimos que las sociedades del Sur deben insertar en su agenda, proyecciones en Ciencia, Tecnología y Sociedad, entre las que el PGH no califica como una prioridad autóctona, aún cuando no descalificamos en su esencia tales megaproyectos, originados en los centros y circuitos propios de la ciencia del NorteAlter brief historical review that informs on the surprising advances of the genetics starting from the discovery of the spiral structure of the DNA, the article centres its attention in the birth of the genetic studies in the United Status of America, the causes and conditions that motivated them, intil ending in the I multinacional Human Genome Project without forgetting the scientific stature of such Project. It is attempted a llok from the perspective of the North-South relationships, remiting us of the more incisive way to the most controversial ethical aspects of the HPG. We argue that in the societies of the South we shoujd be in charge of organizing hierchically the main bioethical problems that we suffer and they are even very distant of those that are in charge of the HGP. We refer that the societies of the South should insert in their calendar

  5. Research projects into the safety of nuclear power plants. Period cover 01. January - 30. June 2017. Progress report

    International Nuclear Information System (INIS)

    2017-01-01

    Within its competence for energy research the Federal Ministry for Economic Affairs and Energy (BMWi) sponsors research projects on the safety of nuclear power plants currently in operation. The objective of these projects is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft fur Anlagen- und Reaktorsicherheit (GRS) gGmbH, by order of the BMWi, continuously issues information on the status of such research projects by publishing semi-annual and annual progress reports within the series of GRS-F-Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are prepared in a standard form by the research organisations themselves as documentation of their progress in work. The progress reports are published by the Project Management Agency/ Authority Support Division of GRS. The reports as of the year 2000 are available in the internet-based information system on results and data of reactor safety research (https://www.grs-fbw.de). The compilation of the reports is classified according to the topic areas of reactor safety research. The reports are arranged in sequence of their project numbers. Ilt has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties.

  6. Short communication: Genomic selection in a crossbred cattle population using data from the Dairy Genetics East Africa Project.

    Science.gov (United States)

    Brown, A; Ojango, J; Gibson, J; Coffey, M; Okeyo, M; Mrode, R

    2016-09-01

    Due to the absence of accurate pedigree information, it has not been possible to implement genetic evaluations for crossbred cattle in African small-holder systems. Genomic selection techniques that do not rely on pedigree information could, therefore, be a useful alternative. The objective of this study was to examine the feasibility of using genomic selection techniques in a crossbred cattle population using data from Kenya provided by the Dairy Genetics East Africa Project. Genomic estimated breeding values for milk yield were estimated using 2 prediction methods, GBLUP and BayesC, and accuracies were calculated as the correlation between yield deviations and genomic breeding values included in the estimation process, mimicking the situation for young bulls. The accuracy of evaluation ranged from 0.28 to 0.41, depending on the validation population and prediction method used. No significant differences were found in accuracy between the 2 prediction methods. The results suggest that there is potential for implementing genomic selection for young bulls in crossbred small-holder cattle populations, and targeted genotyping and phenotyping should be pursued to facilitate this. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  7. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project.

    Science.gov (United States)

    Birney, Ewan; Stamatoyannopoulos, John A; Dutta, Anindya; Guigó, Roderic; Gingeras, Thomas R; Margulies, Elliott H; Weng, Zhiping; Snyder, Michael; Dermitzakis, Emmanouil T; Thurman, Robert E; Kuehn, Michael S; Taylor, Christopher M; Neph, Shane; Koch, Christoph M; Asthana, Saurabh; Malhotra, Ankit; Adzhubei, Ivan; Greenbaum, Jason A; Andrews, Robert M; Flicek, Paul; Boyle, Patrick J; Cao, Hua; Carter, Nigel P; Clelland, Gayle K; Davis, Sean; Day, Nathan; Dhami, Pawandeep; Dillon, Shane C; Dorschner, Michael O; Fiegler, Heike; Giresi, Paul G; Goldy, Jeff; Hawrylycz, Michael; Haydock, Andrew; Humbert, Richard; James, Keith D; Johnson, Brett E; Johnson, Ericka M; Frum, Tristan T; Rosenzweig, Elizabeth R; Karnani, Neerja; Lee, Kirsten; Lefebvre, Gregory C; Navas, Patrick A; Neri, Fidencio; Parker, Stephen C J; Sabo, Peter J; Sandstrom, Richard; Shafer, Anthony; Vetrie, David; Weaver, Molly; Wilcox, Sarah; Yu, Man; Collins, Francis S; Dekker, Job; Lieb, Jason D; Tullius, Thomas D; Crawford, Gregory E; Sunyaev, Shamil; Noble, William S; Dunham, Ian; Denoeud, France; Reymond, Alexandre; Kapranov, Philipp; Rozowsky, Joel; Zheng, Deyou; Castelo, Robert; Frankish, Adam; Harrow, Jennifer; Ghosh, Srinka; Sandelin, Albin; Hofacker, Ivo L; Baertsch, Robert; Keefe, Damian; Dike, Sujit; Cheng, Jill; Hirsch, Heather A; Sekinger, Edward A; Lagarde, Julien; Abril, Josep F; Shahab, Atif; Flamm, Christoph; Fried, Claudia; Hackermüller, Jörg; Hertel, Jana; Lindemeyer, Manja; Missal, Kristin; Tanzer, Andrea; Washietl, Stefan; Korbel, Jan; Emanuelsson, Olof; Pedersen, Jakob S; Holroyd, Nancy; Taylor, Ruth; Swarbreck, David; Matthews, Nicholas; Dickson, Mark C; Thomas, Daryl J; Weirauch, Matthew T; Gilbert, James; Drenkow, Jorg; Bell, Ian; Zhao, XiaoDong; Srinivasan, K G; Sung, Wing-Kin; Ooi, Hong Sain; Chiu, Kuo Ping; Foissac, Sylvain; Alioto, Tyler; Brent, Michael; Pachter, Lior; Tress, Michael L; Valencia, Alfonso; Choo, Siew Woh; Choo, Chiou Yu; Ucla, Catherine; Manzano, Caroline; Wyss, Carine; Cheung, Evelyn; Clark, Taane G; Brown, James B; Ganesh, Madhavan; Patel, Sandeep; Tammana, Hari; Chrast, Jacqueline; Henrichsen, Charlotte N; Kai, Chikatoshi; Kawai, Jun; Nagalakshmi, Ugrappa; Wu, Jiaqian; Lian, Zheng; Lian, Jin; Newburger, Peter; Zhang, Xueqing; Bickel, Peter; Mattick, John S; Carninci, Piero; Hayashizaki, Yoshihide; Weissman, Sherman; Hubbard, Tim; Myers, Richard M; Rogers, Jane; Stadler, Peter F; Lowe, Todd M; Wei, Chia-Lin; Ruan, Yijun; Struhl, Kevin; Gerstein, Mark; Antonarakis, Stylianos E; Fu, Yutao; Green, Eric D; Karaöz, Ulaş; Siepel, Adam; Taylor, James; Liefer, Laura A; Wetterstrand, Kris A; Good, Peter J; Feingold, Elise A; Guyer, Mark S; Cooper, Gregory M; Asimenos, George; Dewey, Colin N; Hou, Minmei; Nikolaev, Sergey; Montoya-Burgos, Juan I; Löytynoja, Ari; Whelan, Simon; Pardi, Fabio; Massingham, Tim; Huang, Haiyan; Zhang, Nancy R; Holmes, Ian; Mullikin, James C; Ureta-Vidal, Abel; Paten, Benedict; Seringhaus, Michael; Church, Deanna; Rosenbloom, Kate; Kent, W James; Stone, Eric A; Batzoglou, Serafim; Goldman, Nick; Hardison, Ross C; Haussler, David; Miller, Webb; Sidow, Arend; Trinklein, Nathan D; Zhang, Zhengdong D; Barrera, Leah; Stuart, Rhona; King, David C; Ameur, Adam; Enroth, Stefan; Bieda, Mark C; Kim, Jonghwan; Bhinge, Akshay A; Jiang, Nan; Liu, Jun; Yao, Fei; Vega, Vinsensius B; Lee, Charlie W H; Ng, Patrick; Shahab, Atif; Yang, Annie; Moqtaderi, Zarmik; Zhu, Zhou; Xu, Xiaoqin; Squazzo, Sharon; Oberley, Matthew J; Inman, David; Singer, Michael A; Richmond, Todd A; Munn, Kyle J; Rada-Iglesias, Alvaro; Wallerman, Ola; Komorowski, Jan; Fowler, Joanna C; Couttet, Phillippe; Bruce, Alexander W; Dovey, Oliver M; Ellis, Peter D; Langford, Cordelia F; Nix, David A; Euskirchen, Ghia; Hartman, Stephen; Urban, Alexander E; Kraus, Peter; Van Calcar, Sara; Heintzman, Nate; Kim, Tae Hoon; Wang, Kun; Qu, Chunxu; Hon, Gary; Luna, Rosa; Glass, Christopher K; Rosenfeld, M Geoff; Aldred, Shelley Force; Cooper, Sara J; Halees, Anason; Lin, Jane M; Shulha, Hennady P; Zhang, Xiaoling; Xu, Mousheng; Haidar, Jaafar N S; Yu, Yong; Ruan, Yijun; Iyer, Vishwanath R; Green, Roland D; Wadelius, Claes; Farnham, Peggy J; Ren, Bing; Harte, Rachel A; Hinrichs, Angie S; Trumbower, Heather; Clawson, Hiram; Hillman-Jackson, Jennifer; Zweig, Ann S; Smith, Kayla; Thakkapallayil, Archana; Barber, Galt; Kuhn, Robert M; Karolchik, Donna; Armengol, Lluis; Bird, Christine P; de Bakker, Paul I W; Kern, Andrew D; Lopez-Bigas, Nuria; Martin, Joel D; Stranger, Barbara E; Woodroffe, Abigail; Davydov, Eugene; Dimas, Antigone; Eyras, Eduardo; Hallgrímsdóttir, Ingileif B; Huppert, Julian; Zody, Michael C; Abecasis, Gonçalo R; Estivill, Xavier; Bouffard, Gerard G; Guan, Xiaobin; Hansen, Nancy F; Idol, Jacquelyn R; Maduro, Valerie V B; Maskeri, Baishali; McDowell, Jennifer C; Park, Morgan; Thomas, Pamela J; Young, Alice C; Blakesley, Robert W; Muzny, Donna M; Sodergren, Erica; Wheeler, David A; Worley, Kim C; Jiang, Huaiyang; Weinstock, George M; Gibbs, Richard A; Graves, Tina; Fulton, Robert; Mardis, Elaine R; Wilson, Richard K; Clamp, Michele; Cuff, James; Gnerre, Sante; Jaffe, David B; Chang, Jean L; Lindblad-Toh, Kerstin; Lander, Eric S; Koriabine, Maxim; Nefedov, Mikhail; Osoegawa, Kazutoyo; Yoshinaga, Yuko; Zhu, Baoli; de Jong, Pieter J

    2007-06-14

    We report the generation and analysis of functional data from multiple, diverse experiments performed on a targeted 1% of the human genome as part of the pilot phase of the ENCODE Project. These data have been further integrated and augmented by a number of evolutionary and computational analyses. Together, our results advance the collective knowledge about human genome function in several major areas. First, our studies provide convincing evidence that the genome is pervasively transcribed, such that the majority of its bases can be found in primary transcripts, including non-protein-coding transcripts, and those that extensively overlap one another. Second, systematic examination of transcriptional regulation has yielded new understanding about transcription start sites, including their relationship to specific regulatory sequences and features of chromatin accessibility and histone modification. Third, a more sophisticated view of chromatin structure has emerged, including its inter-relationship with DNA replication and transcriptional regulation. Finally, integration of these new sources of information, in particular with respect to mammalian evolution based on inter- and intra-species sequence comparisons, has yielded new mechanistic and evolutionary insights concerning the functional landscape of the human genome. Together, these studies are defining a path for pursuit of a more comprehensive characterization of human genome function.

  8. Project Management Plan/Progress Report UT/GTKS Training Program Development for Commercial Building Operators

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2013-03-31

    Universidad del Turabo (UT), in a collaborative effort with Global Turn Key Services, Inc. (GTKS), proposed to develop a training program and a commercialization plan for the development of Commercial Building Operators (CBOs). The CBOs will operate energy efficient buildings to help maintain existing buildings up to their optimal energy performance level, and ensure that net-zero-energy buildings continuously operate at design specifications, thus helping achieve progress towards meeting BTP Strategic Goals of creating technologies and design approaches that enable net-zero-energy buildings at low incremental costs by 2025. The proposed objectives were then: (1) Develop a Commercial Building Operator (CBO) training program and accreditation that will in turn provide a certification to participants recognized by Accreditation Boards such as the North American Board of Certified Energy Practitioners (NABCEP) and Leadership in Energy & Environmental Designs (LEED). (2) Develop and implement a commercialization and sustainability plan that details marketing, deployment, financial characterization, job placement, and other goals required for long-term sustainability of the project after the funding period. (3) After program development and deployment, provide potential candidates with the knowledge and skill sets to obtain employment in the commercial building green energy (net-zero-energy building) job market. The developed CBO training program will focus on providing skills for participants, such as displaced and unemployed workers, to enter the commercial building green energy (net-zeroenergy building) job market. This course was designed to allow a participant with minimal to no experience in commercial building green technology to obtain the required skill sets to enter the job market in as little as 12 weeks of intensive multi-faceted learning. After completion of the course, the CBO staff concluded the participant will meet minimum established accreditation

  9. A nationwide quality improvement project to accelerate Ghana's progress toward Millennium Development Goal Four: design and implementation progress.

    Science.gov (United States)

    Twum-Danso, Nana A Y; Akanlu, George B; Osafo, Enoch; Sodzi-Tettey, Sodzi; Boadu, Richard O; Atinbire, Solomon; Adondiwo, Ane; Amenga-Etego, Isaac; Ashagbley, Francis; Boadu, Eric A; Dasoberi, Ireneous; Kanyoke, Ernest; Yabang, Elma; Essegbey, Ivan T; Adjei, George A; Buckle, Gilbert B; Awoonor-Williams, J Koku; Nang-Beifubah, Alexis; Twumasi, Akwasi; McCannon, C Joseph; Barker, Pierre M

    2012-12-01

    The gap between evidence-based guidelines and practice of care is reflected, in low- and middle-income countries, by high rates of maternal and child mortality and limited effectiveness of large-scale programing to decrease those rates. We designed a phased, rapid, national scale-up quality improvement (QI) intervention to accelerate the achievement of Millennium Development Goal Four in Ghana. Our intervention promoted systems thinking, active participation of managers and frontline providers, generation and testing of local change ideas using iterative learning from transparent district and local data, local ownership and sustainability. After 50 months of implementation, we have completed two prototype learning phases and have begun regional spread phases to all health facilities in all 38 districts of the three northernmost regions and all 29 Catholic hospitals in the remaining regions of the country. To accelerate the spread of improvement, we developed 'change packages' of rigorously tested process changes along the continuum of care from pregnancy to age 5 in both inpatient and outpatient settings. The primary successes for the project so far include broad and deep adoption of QI by local stakeholders for improving system performance, widespread capacitation of leaders, managers and frontline providers in QI methods, incorporation of local ideas into change packages and successful scale-up to approximately 25% of the country's districts in 3 years. Implementation challenges include variable leadership uptake and commitment at the district level, delays due to recruiting and scheduling barriers, weak data systems and repeated QI training due to high staff turnover.

  10. The Agricultural Model Intercomparison and Improvement Project (AgMIP): Progress and Preliminary Results

    Science.gov (United States)

    Rosenzweig, C.

    2011-12-01

    The Agricultural Model Intercomparison and Improvement Project (AgMIP) is a distributed climate-scenario simulation exercise for historical model intercomparison and future climate change conditions with participation of multiple crop and agricultural trade modeling groups around the world. The goals of AgMIP are to improve substantially the characterization of risk of hunger and world food security due to climate change and to enhance adaptation capacity in both developing and developed countries. Recent progress and the current status of AgMIP will be presented, highlighting three areas of activity: preliminary results from crop pilot studies, outcomes from regional workshops, and emerging scientific challenges. AgMIP crop modeling efforts are being led by pilot studies, which have been established for wheat, maize, rice, and sugarcane. These crop-specific initiatives have proven instrumental in testing and contributing to AgMIP protocols, as well as creating preliminary results for aggregation and input to agricultural trade models. Regional workshops are being held to encourage collaborations and set research activities in motion for key agricultural areas. The first of these workshops was hosted by Embrapa and UNICAMP and held in Campinas, Brazil. Outcomes from this meeting have informed crop modeling research activities within South America, AgMIP protocols, and future regional workshops. Several scientific challenges have emerged and are currently being addressed by AgMIP researchers. Areas of particular interest include geospatial weather generation, ensemble methods for climate scenarios and crop models, spatial aggregation of field-scale yields to regional and global production, and characterization of future changes in climate variability.

  11. Greenhouse gas emission trends and projections in Europe 2011. Tracking progress towards Kyoto and 2020 targets

    Energy Technology Data Exchange (ETDEWEB)

    Busche, J.; Scheffler, M.; Graichen, V. (Umweltbundesamt, Vienna (Austria)) (and others)

    2011-10-15

    At the end of 2010, the EU-15 was on track to achieve its Kyoto target but three EU-15 Member States (Austria, Italy and Luxembourg) were not on track to meet their burden-sharing targets. These countries must therefore seriously consider further action to ensure compliance, in particular revising their plans on using flexible mechanisms. Among the EEA member countries outside the EU, Liechtenstein and Switzerland were not on track to achieve their Kyoto target at the end of 2009. All other European countries are on track to meet their targets, either based on domestic emissions only or with the assistance of Kyoto mechanisms. The economic recession had a significant impact on the EU's total greenhouse gas (GHG) emission trends but a more limited effect on progress towards Kyoto targets. This is because emissions in the sectors covered by the EU Emissions Trading Scheme (ETS), which were most affected by the crisis, do not affect Kyoto compliance once ETS caps have been set. With existing national measures, Member States do not project enough emission reductions for the EU to meet its unilateral 20 % reduction commitment in 2020. Additional measures currently planned by Member States will help further reduce emissions but will be insufficient to achieve the important emission cuts needed in the longer term. By 2020 Member States must enhance their efforts to reduce emissions in non-EU ETS sectors, such as the residential, transport or agriculture sectors, where legally binding national targets have been set under the EU's 2009 climate and energy package. (Author)

  12. The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation

    Science.gov (United States)

    The establishment of the epigenetic mark H4K20me1 (monomethylation of H4K20) by PR-Set7 during G2/M directly impacts S-phase progression and genome stability. However, the mechanisms involved in the regulation of this event are not well understood. Here we show that SirT2 regulates H4K20me1 depositi...

  13. A change in strategy for a CERCLA Removal Action Demolition Project in progress results in overall project enhancements

    International Nuclear Information System (INIS)

    Albertin, M.; Nichols, R.M.; Edwards, D.T.

    1995-01-01

    This paper discusses changes made in a demolition project at the Fernald Environmental Management Project (FEMP), a site on the National Priorities list (NPL), owned by the Department of Energy. The project, to demolish fourteen uranium ore silos and their structure, was based on a Removal Action Work Plan, submitted and approved by the United States Environmental Protection Agency (USEPA), that integrated Comprehensive Environmental Response Compensation and Liability Act (CERCLA) requirements to remove the source of contamination and threat to public health and the environment. After the demolition contractor defaulted at 30% complete, completion of the project by the USEPA deadline was threatened. The recovery plan included re-evaluation of project documents in addition to the schedule. It was determined that re-interpretation of the removal action criteria, including design and Removal Action Work Plan, would eliminate road-blocks, and optimize resources, resulting in project completion by the original deadline even after lost-time in mobilizing another contractor. This presentation will discuss the open-quotes lessons learnedclose quotes by the project team and illustrate how simplification of construction methods resulted in enhancements to the environmental controls, improved material handing, and created a safer work environment

  14. Understanding the Human Genome Project: Using Stations to Provide a Comprehensive Overview

    Science.gov (United States)

    Soto, Julio G.

    2005-01-01

    A lesson was designed for lower division general education, non-major biology lecture-only course that included the historical and scientific context, some of the skills used to study the human genome, results, conclusions and ethical consideration. Students learn to examine and compare the published Human Genome maps, and employ the strategies…

  15. Data Mining Approaches for Genomic Biomarker Development: Applications Using Drug Screening Data from the Cancer Genome Project and the Cancer Cell Line Encyclopedia.

    Directory of Open Access Journals (Sweden)

    David G Covell

    Full Text Available Developing reliable biomarkers of tumor cell drug sensitivity and resistance can guide hypothesis-driven basic science research and influence pre-therapy clinical decisions. A popular strategy for developing biomarkers uses characterizations of human tumor samples against a range of cancer drug responses that correlate with genomic change; developed largely from the efforts of the Cancer Cell Line Encyclopedia (CCLE and Sanger Cancer Genome Project (CGP. The purpose of this study is to provide an independent analysis of this data that aims to vet existing and add novel perspectives to biomarker discoveries and applications. Existing and alternative data mining and statistical methods will be used to a evaluate drug responses of compounds with similar mechanism of action (MOA, b examine measures of gene expression (GE, copy number (CN and mutation status (MUT biomarkers, combined with gene set enrichment analysis (GSEA, for hypothesizing biological processes important for drug response, c conduct global comparisons of GE, CN and MUT as biomarkers across all drugs screened in the CGP dataset, and d assess the positive predictive power of CGP-derived GE biomarkers as predictors of drug response in CCLE tumor cells. The perspectives derived from individual and global examinations of GEs, MUTs and CNs confirm existing and reveal unique and shared roles for these biomarkers in tumor cell drug sensitivity and resistance. Applications of CGP-derived genomic biomarkers to predict the drug response of CCLE tumor cells finds a highly significant ROC, with a positive predictive power of 0.78. The results of this study expand the available data mining and analysis methods for genomic biomarker development and provide additional support for using biomarkers to guide hypothesis-driven basic science research and pre-therapy clinical decisions.

  16. Progress report projects in the field of nuclear safety sponsered by the Federal Minister for Research and Technology

    International Nuclear Information System (INIS)

    1980-03-01

    Investigations on the safety of Light Water Reactors (LWR) being performed in the framework of the Research Program Reactor Safety (RS-Projects) are sponsored by the BMFT (Federal Minister for Research and Technology), Bundesminister fuer Forschung und Technologie. Objective of this program is to investigate in greater detail the safety margins of nuclear energy plants and their systems and the further development of safety technology. Besides the investigations of LWR tasks first projects on the safety of FBR type reactors are sponsored by the BMFT. The GRS (Reactor Safety Association), Gesellschaft fuer Reaktorsicherheit mbH, by order of the BMFT, informs continuously of the status of such investigations by means of quarterly and annually publication of progress reports within the series GRS-F-Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about objectives, the work performed, the results, the next steps of the work etc. The individual reports are prepared in a standard form by the contractors themselves as a documentation of their progress in work at the GRS, within the framework of general information of the progress in reactor safety research. The individual reports are arranged according to the amended LWR Safety Research Program of the BMFT. Another table contents uses the same classification system as applied in the Nuclear Safety Index of the CEC and the OECD. (orig./HP) [de

  17. Thorough analysis of unorthodox ABO deletions called by the 1000 Genomes project.

    Science.gov (United States)

    Möller, M; Hellberg, Å; Olsson, M L

    2018-02-01

    ABO remains the clinically most important blood group system, but despite earlier extensive research, significant findings are still being made. The vast majority of catalogued ABO null alleles are based on the c.261delG polymorphism. Apart from c.802G>A, other mechanisms for O alleles are rare. While analysing the data set from the 1000 Genomes (1000G) project, we encountered two previously uncharacterized deletions, which needed further exploration. The Erythrogene database, complemented with bioinformatics software, was used to analyse ABO in 2504 individuals from 1000G. DNA samples from selected 1000G donors and African blood donors were examined by allele-specific PCR and Sanger sequencing to characterize predicted deletions. A 5821-bp deletion encompassing exons 5-7 was called in twenty 1000G individuals, predominantly Africans. This allele was confirmed and its exact deletion point defined by bioinformatic analyses and in vitro experiments. A PCR assay was developed, and screening of African samples revealed three donors heterozygous for this deletion, which was thereby phenotypically established as an O allele. Analysis of upstream genetic markers indicated an ancestral origin from ABO*O.01.02. We estimate this deletion as the 3rd most common mechanism behind O alleles. A 24-bp deletion was called in nine individuals and showed greater diversity regarding ethnic distribution and allelic background. It could neither be confirmed by in silico nor in vitro experiments. A previously uncharacterized ABO deletion among Africans was comprehensively mapped and a genotyping strategy devised. The false prediction of another deletion emphasizes the need for cautious interpretation of NGS data and calls for strict validation routines. © 2017 International Society of Blood Transfusion.

  18. Progress report of the CEC project Rodos system development. Period: 1 september 92-31 august 93

    International Nuclear Information System (INIS)

    Marchand, O.

    1994-01-01

    Within the context of the Radioprotection program of the CEC, the RODOS project (Real-time On-line DecisiOn Support system) aims at the development of a decision support system for nuclear emergencies. RODOS involves 22 research teams, divided in 4 sub-projects: 'Meteorology and Atmospheric Dispersion, 'System Development', 'Decision Aiding Techniques'. The fourth sub-project is a Joint Study Project of the Agreement between CEC ad the CIS republics. EDF is working in the 'System Development' sub-project and namely in the 'training' group. This group aims at the creation of a specific training course for health physics managers, based on RODOS. This note reproduces the progress report of the 'Development System' project. The reporting period is: September 92 - August 93. Progress bas been made within the reporting period in the: - development of data assimilation methods incorporating both monitoring data and model predictions for obtaining consistent pictures of the environmental contamination and the source term ; - improvement and extension of the modules ATSTEP-CORA (atmospheric dispersion and deposition), EMERSIM (simulation of emergency actions), ECOAMOR (exposure pathways and dose calculation) and FRODO (simulation of relocation and agricultural countermeasures) ; - preparation of training courses using RODOS as illustrative tool ; - extension of the functions of the RODOS operating system OSY, in particular of RoGIS, its geographical information system. (author). 2 figs

  19. Leaching Behavior of Coal Combustion Products and the Environmental Implication in Road Construction : Project Progress Report

    Science.gov (United States)

    2009-01-26

    This is the third annual report for Project #043352. Project #043352 is designed to establish the leaching characteristics for several different types of ash using a relatively rigorous leaching procedure developed by Kosson et al (2002) for a wide r...

  20. Projects at the Western Environmental Technology Office. Quarterly technical progress report, April 1--June 30, 1995

    International Nuclear Information System (INIS)

    1995-01-01

    This report contains brief outlines of the multiple projects under the responsibility of the Western Environmental Technology Office in Butte Montana. These projects include biomass remediation, remediation of contaminated soils, mine waste technology, and several other types of remediation

  1. Transportation Infrastructure: Progress on and Challenges to Central Artery/Tunnel Project's Cost and Financing

    Science.gov (United States)

    1997-07-01

    The Central Artery/Tunnel project in Boston, Massachusetts--one of the largest, most complex, and most expensive highway construction projects ever undertaken--is well under way, with contracts worth nearly $8 billion either completed or awarded. Con...

  2. Progress and results of the project ENETRAP II: European network of education and training in radiation protection

    International Nuclear Information System (INIS)

    Marco, M.; Llorente Herranz, C.; Coeck, M.; Livosi, P.; Massiot, P.; Moebius, S.

    2013-01-01

    The CIEMAT has participated in a number of working groups and has led the WP 6 for the creation of a database of events of specific training the RPE and the RPO taking into account aspects developed in schemes of defined training. The database includes providers and job training opportunities. Is a tool that will serve as a mechanism for comparison with established standards of training in the project.The project has made great progress in the implementation of the new directive at European level. (Author)

  3. The Micronutrient Genomics Project: A community-driven knowledge base for micronutrient research

    NARCIS (Netherlands)

    Ommen, B. van; El-Sohemy, A.; Hesketh, J.; Kaput, J.; Fenech, M.; Evelo, C.T.; McArdle, H.J.; Bouwman, J.; Lietz, G.; Mathers, J.C.; Fairweather-Tait, S.; Kranen, H. van; Elliott, R.; Wopereis, S.; Ferguson, L.R.; Méplan, C.; Perozzi, G.; Allen, L.; Rivero, D.

    2010-01-01

    Micronutrients influence multiple metabolic pathways including oxidative and inflammatory processes. Optimum micronutrient supply is important for the maintenance of homeostasis in metabolism and, ultimately, for maintaining good health. With advances in systems biology and genomics technologies, it

  4. Controlling our destinies: Historical, philosophical, social and ethical perspectives on the Human Genome Project: Final report, July 1, 1995-June 30, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Sloan, P.R.

    1996-09-25

    This report briefly describes the efforts by the organizing committee in preparation for the conference entitled Controlling Our Destinies: Historical, Philosophical, Social, and Ethical Perspectives on the Human Genome Project. The conference was held October 5-8, 1995.

  5. Yucca Mountain site characteriztion project bibliography. Progress Report, 1994--1995

    International Nuclear Information System (INIS)

    1996-08-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project which was added to the Department of Energy's Energy Science and Technology Database from January 1, 1994, through December 31, 1995. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization's list. Another section contains information about publications on the Energy Science and Technology database which were not sponsored by the project but have some relevance to it

  6. Research Progress in Mathematical Analysis of Map Projection by Computer Algebra

    Directory of Open Access Journals (Sweden)

    BIAN Shaofeng

    2017-10-01

    Full Text Available Map projection is an important component of modern cartography, and involves many fussy mathematical analysis processes, such as the power series expansions of elliptical functions, differential of complex and implicit functions, elliptical integral and the operation of complex numbers. The derivation of these problems by hand not only consumes much time and energy but also makes mistake easily, and sometimes can not be realized at all because of the impossible complexity. The research achievements in mathematical analysis of map projection by computer algebra are systematically reviewed in five aspects, i.e., the symbolic expressions of forward and inverse solution of ellipsoidal latitudes, the direct transformations between map projections with different distortion properties, expressions of Gauss projection by complex function, mathematical analysis of oblique Mercator projection, polar chart projection with its transformation. Main problems that need to be further solved in this research field are analyzed. It will be helpful to promote the development of map projection.

  7. Yucca Mountain site characteriztion project bibliography. Progress Report, 1994--1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    Following a reorganization of the Office of Civilian Radioactive Waste Management in 1990, the Yucca Mountain Project was renamed Yucca Mountain Site Characterization Project. The title of this bibliography was also changed to Yucca Mountain Site Characterization Project Bibliography. Prior to August 5, 1988, this project was called the Nevada Nuclear Waste Storage Investigations. This bibliography contains information on this ongoing project which was added to the Department of Energy`s Energy Science and Technology Database from January 1, 1994, through December 31, 1995. The bibliography is categorized by principal project participating organization. Participant-sponsored subcontractor reports, papers, and articles are included in the sponsoring organization`s list. Another section contains information about publications on the Energy Science and Technology database which were not sponsored by the project but have some relevance to it.

  8. Contributing to Tumor Molecular Characterization Projects with a Global Impact | Office of Cancer Genomics

    Science.gov (United States)

    My name is Nicholas Griner and I am the Scientific Program Manager for the Cancer Genome Characterization Initiative (CGCI) in the Office of Cancer Genomics (OCG). Until recently, I spent most of my scientific career working in a cancer research laboratory. In my postdoctoral training, my research focused on identifying novel pathways that contribute to both prostate and breast cancers and studying proteins within these pathways that may be targeted with cancer drugs.

  9. Progress report of the ECN contribution to the experiments in the HAW project

    International Nuclear Information System (INIS)

    Duijves, K.A.; Vonka, V.

    1990-12-01

    This progress report documents the progress of the ECN contribution in the High Active Waste experiment, a large-scale in situ test being performed underground at the Asse salt mine in Remlingen FRG, during the period April 1st through June 30th, 1990. In ch. 2 a general overview is given of the experiment development. Problems and solutions are discussed in ch. 3. (author). 4 refs.; 10 figs

  10. Walla Walla River Fish Passage Operations Project : Annual Progress Report October 2007 - September 2008.

    Energy Technology Data Exchange (ETDEWEB)

    Bronson, James P.; Duke, Bill; Loffink, Ken

    2008-12-30

    In the late 1990s, the Confederated Tribes of the Umatilla Indian Reservation, Oregon Department of Fish and Wildlife, and Washington Department of Fish and Wildlife, along with many other agencies, began implementing fisheries restoration activities in the Walla Walla Basin. An integral part of these efforts is to alleviate the inadequate fish migration conditions in the basin. Migration concerns are being addressed by removing diversion structures, constructing fish passage facilities, implementing minimum instream flow requirements, and providing trap and haul efforts when needed. The objective of the Walla Walla River Fish Passage Operations Project is to increase the survival of migrating adult and juvenile salmonids in the Walla Walla River basin. The project is responsible for coordinating operation and maintenance of ladders, screen sites, bypasses, trap facilities, and transportation equipment. In addition, the project provides technical input on passage and trapping facility design, operation, and criteria. Operation of the various passage facilities and passage criteria guidelines are outlined in an annual operations plan that the project develops. Beginning in March of 2007, two work elements from the Walla Walla Fish Passage Operations Project were transferred to other projects. The work element Enumeration of Adult Migration at Nursery Bridge Dam is now conducted under the Walla Walla Basin Natural Production Monitoring and Evaluation Project and the work element Provide Transportation Assistance is conducted under the Umatilla Satellite Facilities Operation and Maintenance Project. Details of these activities can be found in those project's respective annual reports.

  11. Genome-derived vaccines.

    Science.gov (United States)

    De Groot, Anne S; Rappuoli, Rino

    2004-02-01

    Vaccine research entered a new era when the complete genome of a pathogenic bacterium was published in 1995. Since then, more than 97 bacterial pathogens have been sequenced and at least 110 additional projects are now in progress. Genome sequencing has also dramatically accelerated: high-throughput facilities can draft the sequence of an entire microbe (two to four megabases) in 1 to 2 days. Vaccine developers are using microarrays, immunoinformatics, proteomics and high-throughput immunology assays to reduce the truly unmanageable volume of information available in genome databases to a manageable size. Vaccines composed by novel antigens discovered from genome mining are already in clinical trials. Within 5 years we can expect to see a novel class of vaccines composed by genome-predicted, assembled and engineered T- and Bcell epitopes. This article addresses the convergence of three forces--microbial genome sequencing, computational immunology and new vaccine technologies--that are shifting genome mining for vaccines onto the forefront of immunology research.

  12. Lake Roosevelt White Sturgeon Recovery Project : Annual Progress Report, January 2003 – March 2004.

    Energy Technology Data Exchange (ETDEWEB)

    Howell, Matthew D.; McLellan, Jason G. [Washington Department of Fish and Wildlife

    2009-07-15

    This report summarizes catch data collected from white sturgeon Acipenser transmontanus in Lake Roosevelt during limited setlining and gill netting activities in the fall of 2003, and documents progress toward development of a U.S. white sturgeon conservation aquaculture program for Lake Roosevelt. From 27-30 October, 42 overnight small mesh gill net sets were made between Marcus and Northport, WA for a total catch of 15 juvenile white sturgeon (275-488 mm FL). All sturgeon captured were of Canadian hatchery origin. These fish had been previously released as sub-yearlings into the Canadian portion (Keenleyside Reach) of the Transboundary Reach of the Columbia River during 2002 and 2003. Most sturgeon (n=14) were caught in the most upstream area sampled (Northport) in low velocity eddy areas. Five fish exhibited pectoral fin deformities (curled or stunted). Growth rates were less than for juvenile sturgeon captured in the Keenleyside Reach but condition factor was similar. Condition factor was also similar to that observed in juvenile sturgeon (ages 1-8) captured in the unimpounded Columbia River below Bonneville Dam between 1987-92. From 10-14 November, 28 overnight setline sets were made in the Roosevelt Reach between the confluence of the Spokane River and Marcus Island for a total catch of 17 white sturgeon (94-213 cm FL). Catch was greatest in the most upstream areas sampled, a distribution similar to that observed during a WDFW setline survey in Lake Roosevelt in 1998. The mean W{sub r} index of 110% for fish captured this year was higher than the mean W{sub r} of 91% for fish captured in 1998. Excellent fish condition hindered surgical examination of gonads as lipid deposits made the ventral body wall very thick and difficult to penetrate with available otoscope specula. Acoustic tags (Vemco model V16 coded pingers, 69 kHz, 48-month life expectancy) were internally applied to 15 fish for subsequent telemetry investigations of seasonal and reproductively

  13. Mirage project. Second summary progress report (Work period January to December 1984)

    International Nuclear Information System (INIS)

    Come, B.

    1985-01-01

    This report summarizes the second year of work (1984) in the CEC project MIRAGE on migration of radionuclides in the geosphere. It complements CEC reports EUR 9304 (Description of the project) and EUR 9543 (Works carried out in 1983) on the same topic

  14. High Performance Parallel Processing Project: Industrial computing initiative. Progress reports for fiscal year 1995

    Energy Technology Data Exchange (ETDEWEB)

    Koniges, A.

    1996-02-09

    This project is a package of 11 individual CRADA`s plus hardware. This innovative project established a three-year multi-party collaboration that is significantly accelerating the availability of commercial massively parallel processing computing software technology to U.S. government, academic, and industrial end-users. This report contains individual presentations from nine principal investigators along with overall program information.

  15. Projected progress in the engineering state-of-the-art. [for aerospace

    Science.gov (United States)

    Nicks, O. W.

    1978-01-01

    Projected advances in discipline areas associated with aerospace engineering are discussed. The areas examined are propulsion and power, materials and structures, aerothermodynamics, and electronics. Attention is directed to interdisciplinary relationships; one example would be the application of communications technology to the solution of propulsion problems. Examples involving projected technology changes are presented, and technology integration and societal effects are considered.

  16. Chernobyl Studies Project - working group 7.0 environmental transport and health effects. Progress report, October 1993--January 1994

    Energy Technology Data Exchange (ETDEWEB)

    Hendrickson, S.M. [ed.

    1994-03-01

    The DOE-funded Chernobyl Studies Project was begun as part of a cooperative agreement between the US and the former USSR, (quote) To develop jointly methods to project rapidly the health effects of any future reactor accident (quote). Most of the initial tasks for this project are completed or near completion. The focus has now turned primarily to the issue of health effects from the Chernobyl accident. Currently, we are extensively engaged in case-control and cohort studies of thyroid diseases among Belarussian children and in the Ukraine. A major part of the effort is providing methods and applications of dose reconstruction and providing support and equipment for the medical teams. This document contains reports on progress in the following task areas: Management; External Dose; Hydrological Transport; Chromosome Painting Dosimetry; Stochastic Effects; Thyroid Studies; and Leukemia Studies.

  17. Technical progress in planning organization of the Ostravo-Karwina coal basin, ''Mining Projects of Ostravo''

    Energy Technology Data Exchange (ETDEWEB)

    Karpeta, B; Kolar, J

    1979-01-01

    Based on the main task of further improvement in labor productivity and improvement in the quality of products, the leading planning organization of the Ostravo-Karwina basin is planning and realizing progressive technological plans based on new equipment. Long-term plans for basin development up to 1990 stipulate a rise in capital investments by 180%, increase in the volume of productivity by 164% with a rise in the number of workers by 142%. Corresponding technical progress in planning is based on an improvement in the system of scientific-technical information, automation and technical equipping of the planning process, improvements in the forms and organizational structure. Organization of specialized research-planning groups to substantiate and to technically-economically evaluate technical progress, and also to develop comprehensive technical assignments is stipulated.

  18. Overview of progress on the improvement projects for the LANSCE accelerator and target facilities

    International Nuclear Information System (INIS)

    Macek, R.J.; Browne, J.; Brun, T.; Donahue, J.B.; Fitzgerald, D.H.; Hoffman, E.; Pynn, R.; Schriber, S.; Weinacht, D.

    1997-01-01

    Three projects have been initiated since 1994 to improve the performance of the accelerator and target facilities for the Los Alamos Neutron Science Center (LANSCE). The LANSCE Reliability Improvement Project (LRIP) was separated into two phases. Phase 1, completed in 1995, targeted near-term improvements to beam reliability and availability that could be completed in one-year's time. Phase 2, now underway and scheduled for completion in May 1998, consists of two projects: (a) implementation of direct H-injection for the Proton Storage Ring (PSR) and (b) an upgrade of the target/moderator system for the short pulse spallation neutron (SPSS) source. The latter will reduce the target change-out time from about 10 months to about three weeks. The third project, the SPSS Enhancement Project, is aimed at increasing the PSR output beam current to 200 microA at 30 Hz and providing up to seven new neutron scattering instruments

  19. A prospective pilot study of genome-wide exome and transcriptome profiling in patients with small cell lung cancer progressing after first-line therapy.

    Directory of Open Access Journals (Sweden)

    Glen J Weiss

    Full Text Available Small cell lung cancer (SCLC that has progressed after first-line therapy is an aggressive disease with few effective therapeutic strategies. In this prospective study, we employed next-generation sequencing (NGS to identify therapeutically actionable alterations to guide treatment for advanced SCLC patients.Twelve patients with SCLC were enrolled after failing platinum-based chemotherapy. Following informed consent, genome-wide exome and RNA-sequencing was performed in a CLIA-certified, CAP-accredited environment. Actionable targets were identified and therapeutic recommendations made from a pharmacopeia of FDA-approved drugs. Clinical response to genomically-guided treatment was evaluated by Response Evaluation Criteria in Solid Tumors (RECIST 1.1.The study completed its accrual goal of 12 evaluable patients. The minimum tumor content for successful NGS was 20%, with a median turnaround time from sample collection to genomics-based treatment recommendation of 27 days. At least two clinically actionable targets were identified in each patient, and six patients (50% received treatment identified by NGS. Two had partial responses by RECIST 1.1 on a clinical trial involving a PD-1 inhibitor + irinotecan (indicated by MLH1 alteration. The remaining patients had clinical deterioration before NGS recommended therapy could be initiated.Comprehensive genomic profiling using NGS identified clinically-actionable alterations in SCLC patients who progressed on initial therapy. Recommended PD-1 therapy generated partial responses in two patients. Earlier access to NGS guided therapy, along with improved understanding of those SCLC patients likely to respond to immune-based therapies, should help to extend survival in these cases with poor outcomes.

  20. A prospective pilot study of genome-wide exome and transcriptome profiling in patients with small cell lung cancer progressing after first-line therapy.

    Science.gov (United States)

    Weiss, Glen J; Byron, Sara A; Aldrich, Jessica; Sangal, Ashish; Barilla, Heather; Kiefer, Jeffrey A; Carpten, John D; Craig, David W; Whitsett, Timothy G

    2017-01-01

    Small cell lung cancer (SCLC) that has progressed after first-line therapy is an aggressive disease with few effective therapeutic strategies. In this prospective study, we employed next-generation sequencing (NGS) to identify therapeutically actionable alterations to guide treatment for advanced SCLC patients. Twelve patients with SCLC were enrolled after failing platinum-based chemotherapy. Following informed consent, genome-wide exome and RNA-sequencing was performed in a CLIA-certified, CAP-accredited environment. Actionable targets were identified and therapeutic recommendations made from a pharmacopeia of FDA-approved drugs. Clinical response to genomically-guided treatment was evaluated by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1. The study completed its accrual goal of 12 evaluable patients. The minimum tumor content for successful NGS was 20%, with a median turnaround time from sample collection to genomics-based treatment recommendation of 27 days. At least two clinically actionable targets were identified in each patient, and six patients (50%) received treatment identified by NGS. Two had partial responses by RECIST 1.1 on a clinical trial involving a PD-1 inhibitor + irinotecan (indicated by MLH1 alteration). The remaining patients had clinical deterioration before NGS recommended therapy could be initiated. Comprehensive genomic profiling using NGS identified clinically-actionable alterations in SCLC patients who progressed on initial therapy. Recommended PD-1 therapy generated partial responses in two patients. Earlier access to NGS guided therapy, along with improved understanding of those SCLC patients likely to respond to immune-based therapies, should help to extend survival in these cases with poor outcomes.

  1. Aspergillus and Penicillium in the Post-genomic Era

    DEFF Research Database (Denmark)

    and a whole genus genome sequencing project in progress for Aspergillus. This book highlights some of the changes in the studies into these fungi, since the availability of genome sequences. The contributions vary from insights in the taxonomy of these genera, use of genomics for forward genetics and genomic......Genome sequencing has affected studies into the biology of all classes of organisms and this is certainly true for filamentous fungi. The level with which biological systems can be studied since the availability of genomes and post-genomic technologies is beyond what most people could have imagined...... previously. The fungal genera Aspergillus and Penicillium contain some species that are amongst the most widely used industrial microorganisms and others that are serious pathogens of plants, animals and humans. These genera are also at the forefront of fungal genomics with many genome sequences available...

  2. Ceramic technology for advanced heat engines project: Semiannual progress report for April through September 1986

    Energy Technology Data Exchange (ETDEWEB)

    1987-03-01

    An assessment of needs was completed, and a five-year project plan was developed with extensive input from private industry. Objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. The project approach includes determining the mechanisms controlling reliability, improving processes for fabricating existing ceramics, developing new materials with increased reliability, and testing these materials in simulated engine environments to confirm reliability. Although this is a generic materials project, the focus is on structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines.

  3. Experimental Physics Division of the Los Alamos Project. Progress report No. 4

    Energy Technology Data Exchange (ETDEWEB)

    1943-09-01

    Included in this semi-monthly report written in 1943 are progress with neutron beams, neutron absorption in enriched materials, equipment operation and maintenance reports of the cyclotron neutron source facility, and instrumentation maintenance activities of individuals in the cyclotron group. (GHT)

  4. Progress report of the ECN contribution to the experiments in the HAW project

    International Nuclear Information System (INIS)

    Duijves, K.A.; Vonka, V.

    1991-08-01

    This report documents the progress of the ECN contribution in the High Active Waste Experiment, a large-scale in-situ test being performed underground in the Asse salt mine, Remlingen, FRG, during the period January 1st through March 31st, 1991. (A.S.). 3 refs.; 10 figs

  5. Coast Guard: Progress Being Made on Deepwater Project, but Risks Remain

    National Research Council Canada - National Science Library

    2001-01-01

    The Coast Guard is in the final stages of planning the largest procurement project in its history the modernization and/or replacement of over 90 cutters and 200 aircraft used for missions beyond 50 miles from shore...

  6. Whitehead Policy Symposium. The Human Genome Project: Science, law, and social change in the 21st century

    Energy Technology Data Exchange (ETDEWEB)

    Nichols, E.K.

    2000-02-17

    Advances in the biomedical sciences, especially in human genomics, will dramatically influence law, medicine, public health, and many other sectors of our society in the decades ahead. The public already senses the revolutionary nature of genomic knowledge. In the US and Europe, we have seen widespread discussions about genetic discrimination in health insurance; privacy issues raised by the proliferation of DNA data banks; the challenge of interpreting new DNA diagnostic tests; changing definitions of what it means to be healthy; and the science and ethics of cloning animals and human beings. The primary goal of the Whitehead/ASLME Policy Symposium was to provide a bridge between the research community and professionals, who were just beginning to grasp the potential impact of new genetic technologies on their fields. The ''Human Genome Project: Science, Law, and Social Change in the 21st Century'' initially was designed as a forum for 300-500 physicians, lawyers, consumers, ethicists, and scientists to explore the impact of new genetic technologies and prepare for the challenges ahead.

  7. Amino acid changes in disease-associated variants differ radically from variants observed in the 1000 genomes project dataset.

    Directory of Open Access Journals (Sweden)

    Tjaart A P de Beer

    Full Text Available The 1000 Genomes Project data provides a natural background dataset for amino acid germline mutations in humans. Since the direction of mutation is known, the amino acid exchange matrix generated from the observed nucleotide variants is asymmetric and the mutabilities of the different amino acids are very different. These differences predominantly reflect preferences for nucleotide mutations in the DNA (especially the high mutation rate of the CpG dinucleotide, which makes arginine mutability very much higher than other amino acids rather than selection imposed by protein structure constraints, although there is evidence for the latter as well. The variants occur predominantly on the surface of proteins (82%, with a slight preference for sites which are more exposed and less well conserved than random. Mutations to functional residues occur about half as often as expected by chance. The disease-associated amino acid variant distributions in OMIM are radically different from those expected on the basis of the 1000 Genomes dataset. The disease-associated variants preferentially occur in more conserved sites, compared to 1000 Genomes mutations. Many of the amino acid exchange profiles appear to exhibit an anti-correlation, with common exchanges in one dataset being rare in the other. Disease-associated variants exhibit more extreme differences in amino acid size and hydrophobicity. More modelling of the mutational processes at the nucleotide level is needed, but these observations should contribute to an improved prediction of the effects of specific variants in humans.

  8. Low cost solar array project. Quarterly progress report, January-March, 1981

    Energy Technology Data Exchange (ETDEWEB)

    1981-01-01

    The engineering design, fabrication, assembly, operation, economic analysis, and process support R and D for an Experimental Process System Development Unit (EPSDU) are presented. The civil construction work was completed and the mechanical bid package is in preparation. The electrical design effort is in progress. Parallel efforts which complement the mechanical design are the process flow diagrams and control instrumentation logic for startup operation and shutdown. These are in progress and will identify all process and utility streams, control systems, and flow logic. The data collection system takes the signals from the instrumentation, translates them into engineering units and finally develops a data report which summarizes all key performance parameters. Cleaning procedures have been established to assure a contamination-free product and inspection visits have been made to the fabricators of specialty equipment.

  9. Geostatistics project of the National Uranium Resources Evaluation Program. Progress report, October 1978--March 1979

    International Nuclear Information System (INIS)

    Beckman, R.J.; Bement, T.R.; Campbell, K.; Howell, J.S.; Wecksung, G.W.; Whitemann, D.E.

    1979-01-01

    During the period covered by this report, research was concentrated on multivariate approaches to the analysis of aerial radiometric data. Two aspects of principal components analysis were the subjects of two publications. The procedures recommended for linear discriminant analysis were revised. Progress was made in overlaying LANDSAT data with aerial radiometric data from the Lubbock quadrangle. Some preliminary results from principal components analysis of the Wind River data were obtained

  10. The Umatilla Basin Natural Production Monitoring and Evaluation Project, 2008 Annual Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Contor, Craig R.; Harris, Robin; King, Marty [Confederated Tribes of the Umatilla Indian Reservation

    2009-06-10

    The Umatilla Basin Natural Production Monitoring and Evaluation Project (UBNPMEP) is funded by Bonneville Power Administration (BPA) as directed by section 4(h) of the Pacific Northwest Electric Power Planning and Conservation Act of 1980 (P.L.96-501). This project is in accordance with and pursuant to measures 4.2A, 4.3C.1, 7.1A.2, 7.1C.3, 7.1C.4 and 7.1D.2 of the Northwest Power Planning Council's (NPPC) Columbia River Basin Fish and Wildlife Program (NPPC 1994). Work was conducted by the Fisheries Program of the Confederated Tribes of the Umatilla Indian Reservation (CTUIR). The UBNPMEP is coordinated with two Oregon Department of Fish and Wildlife (ODFW) research projects that also monitor and evaluate the success of the Umatilla Fisheries Restoration Plan. This project deals with the natural production component of the plan, and the ODFW projects evaluate hatchery operations (project No. 1990-005-00, Umatilla Hatchery M & E) and smolt outmigration (project No. 1989-024-01, Evaluation of Juvenile Salmonid Outmigration and Survival in the Lower Umatilla River). Collectively these three projects monitor and evaluate natural and hatchery salmonid production in the Umatilla River Basin. The need for natural production monitoring has been identified in multiple planning documents including Wy-Kan-Ush-Mi Wa-Kish-Wit Volume I, 5b-13 (CRITFC 1996), the Umatilla Hatchery Master Plan (CTUIR & ODFW 1990), the Umatilla Basin Annual Operation Plan, the Umatilla Subbasin Summary (CTUIR & ODFW 2001), the Subbasin Plan (CTUIR & ODFW 2004), and the Comprehensive Research, Monitoring, and Evaluation Plan (CTUIR and ODFW 2006). Natural production monitoring and evaluation is also consistent with Section III, Basinwide Provisions, Strategy 9 of the 2000 Columbia River Basin Fish and Wildlife Program (NPPC 1994, NPCC 2004). The Umatilla Basin M&E plan developed along with efforts to restore natural populations of spring and fall Chinook salmon, (Oncorhynchus tshawytsha), coho

  11. SERI Solar Radiation Resource Assessment Project: Fiscal Year 1990 Annual Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Riordan, C; Maxwell, E; Stoffel, T; Rymes, M; Wilcox, S

    1991-07-01

    The purpose of the Solar Radiation Resource Project is to help meet the needs of the public, government, industry, and utilities for solar radiation data, models, and assessments as required to develop, design, deploy, and operate solar energy conversion systems. The project scientists produce information on the spatial (geographic), temporal (hourly, daily, and seasonal), and spectral (wavelength distribution) variability of solar radiation at different locations in the United States. Resources committed to the project in FY 1990 supported about four staff members, including part-time administrative support. With these resources, the staff must concentrate on solar radiation resource assessment in the United States; funds do not allow for significant efforts to respond to a common need for improved worldwide data. 34 refs., 21 figs., 6 tabs.

  12. The SHARPn project on secondary use of Electronic Medical Record data: progress, plans, and possibilities.

    Science.gov (United States)

    Chute, Christopher G; Pathak, Jyotishman; Savova, Guergana K; Bailey, Kent R; Schor, Marshall I; Hart, Lacey A; Beebe, Calvin E; Huff, Stanley M

    2011-01-01

    SHARPn is a collaboration among 16 academic and industry partners committed to the production and distribution of high-quality software artifacts that support the secondary use of EMR data. Areas of emphasis are data normalization, natural language processing, high-throughput phenotyping, and data quality metrics. Our work avails the industrial scalability afforded by the Unstructured Information Management Architecture (UIMA) from IBM Watson Research labs, the same framework which underpins the Watson Jeopardy demonstration. This descriptive paper outlines our present work and achievements, and presages our trajectory for the remainder of the funding period. The project is one of the four Strategic Health IT Advanced Research Projects (SHARP) projects funded by the Office of the National Coordinator in 2010.

  13. Passive ALWR safety: the ALPHA project at Switzerland's PSI - a progress report

    International Nuclear Information System (INIS)

    Yadigaroglu, G.; Varadi, G.; Dreier, J.

    1995-01-01

    The Paul Scherrer Institute (PSI) initiated the ALPHA project in 1991 for the experimental and analytical investigation of the long-term decay heat removal from the containment of the next generation of 'passive' advanced light water reactors (ALWRs). The dynamic containment response to such systems, as well as containment phenomena, are investigated. The ALPHA project includes integral system tests in the large-scale (1:25 in volume) PANDA facility; the smaller-scale separate effects LINX series of tests related to various passive containment mixing, stratification, and condensation phenomena in the presence of non-condensable gases; the AIDA tests on the behavior of aerosols in passive containment cooling systems (PCCS); and supporting analytical work. The project has been, so far, directed mainly to the investigation of the General Electric simplified boiling water reactor (SBWR) PCCS and related phenomena. (author) 2 figs., 4 refs

  14. Development of a wind farm noise propagation prediction model - project progress to date

    International Nuclear Information System (INIS)

    Robinson, P.; Bullmore, A.; Bass, J.; Sloth, E.

    1998-01-01

    This paper describes a twelve month measurement campaign which is part of a European project (CEC Project JOR3-CT95-0051) with the aim to substantially reduce the uncertainties involved in predicting environmentally radiated noise levels from wind farms (1). This will be achieved by comparing noise levels measure at varying distances from single and multiple sources over differing complexities of terrain with those predicted using a number of currently adopted sound propagation models. Specific objectives within the project are to: establish the important parameters controlling the propagation of wind farm noise to the far field; develop a planning tool for predicting wind farm noise emission levels under practically encountered conditions; place confidence limits on the upper and lower bounds of the noise levels predicted, thus enabling developers to quantify the risk whether noise emission from wind farms will cause nuisance to nearby residents. (Author)

  15. Ceramic technology for advanced heat engines project. Semiannual progress report, April-September 1985

    Energy Technology Data Exchange (ETDEWEB)

    1986-05-01

    An assessment of needs was completed, and a five-year project plan was developed with input from private industry. Objective is to develop the industrial technology base required for reliable ceramics for application in advanced automotive heat engines. Focus is on structural ceramics for advanced gas turbine and diesel engines, ceramic bearings and attachments, and ceramic coatings for thermal barrier and wear applications in these engines. The work described in this report is organized according to the following WBS project elements: management and coordination; materials and processing (monolithics, ceramic composites, thermal and wear coatings, joining); materials design methodology (contact interfaces, new concepts); data base and life prediction (time-dependent behavior, environmental effects, fracture mechanics, NDE development); and technology transfer. This report includes contributions from all currently active project participants.

  16. Geothermal direct-heat utilization assistance. Quarterly project progress report, July 1996--September 1996. Federal Assistance Program

    Energy Technology Data Exchange (ETDEWEB)

    Lienau, P.

    1996-11-01

    This report summarizes geothermal technical assistance, R&D and technology transfer activities of the Geo-Heat Center at Oregon Institute of Technology for the fourth quarter of FY-96. It describes 152 contacts with parties during this period related to technical assistance with geothermal direct heat projects. Areas dealt with include geothermal heat pumps, space heating, greenhouses, aquaculture, equipment, economics and resources. Research activities are summarized on greenhouse peaking. Outreach activities include the publication of a geothermal direct use Bulletin, dissemination of information, geothermal library, technical papers and seminars, and progress monitor reports on geothermal resources and utilization.

  17. Trends and projections in Europe 2013. Tracking progress towards Europe's climate and energy targets until 2020

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-10-01

    This report provide an assessment of the progress of the EU and European countries towards achieving their climate mitigation and energy policy objectives. These targets include international commitments pursuant the KP and the EU 2020 commitment to reduce by 20 % greenhouse gas (GHG) emissions compared to 1990, to create 20 % of energy consumption from renewables and to increase energy efficiency by 20 %. The assessment is based on GHG data for the period 2008-2012, including recent estimates of proxy 2012 GHG emissions, GHG projections until 2020 submitted by Member States in 2013, as well as energy statistics until 2011. (Author)

  18. Progress on the Design of the Storage Ring Vacuum System for the Advanced Photon Source Upgrade Project

    Energy Technology Data Exchange (ETDEWEB)

    Stillwell, B.; Billett, B.; Brajuskovic, B.; Carter, J.; Kirkus, E.; Lale, M.; Lerch, J.; Noonan, J.; O' Neill, M.; Rocke, B.; Suthar, K.; Walters, D.; Wiemerslage, G.; Zientek, J.

    2017-06-20

    Recent work on the design of the storage ring vacuum system for the Advanced Photon Source Upgrade project (APS-U) includes: revising the vacuum system design to accommodate a new lattice with reverse bend magnets, modifying the designs of vacuum chambers in the FODO sections for more intense incident synchrotron radiation power, modifying the design of rf-shielding bellows liners for better performance and reliability, modifying photon absorber designs to make better use of available space, and integrated planning of components needed in the injection, extraction and rf cavity straight sections. An overview of progress in these areas is presented.

  19. HIFS VNL Monthly Progress Report Preparation for NDCX-II Project

    International Nuclear Information System (INIS)

    Logan, Grant; Kwan, Joe; Barnard, John; Friedman, Alex; Gilson, Erik; Leitner, Matthaeus; Waldron, Will; Bieniosek, Frank

    2009-01-01

    In preparation for the project and for anticipated review in August, the HIFS-VNL hosted an NDCX-II Advisory Meeting at LBNL on May 27, 2009. A number of experts in accelerator physics, engineering, and construction were asked to visit for a full day, listen to presentations on the project, its goals, and its status, and to offer their advice on how best to proceed, what topics needed attention, and what technical options seemed most attractive to them. This was a productive meeting, and the Committee's comments will provide useful guidance.

  20. Research projects into the safety of nuclear power plants. Period covered: 01. July - 31. December 2004. Progress report

    International Nuclear Information System (INIS)

    2004-01-01

    Within its competence for energy research, the Bundesministerium fuer Wirtschaft und Technology (BMWi) (Federal Ministry of Economics and Technology) sponsors investigations into the safety of nuclear power plants. The objective of these investigations is to provide fundamental knowledge, procedures and methods to contribute to realistic safety assessments of nuclear installations, to the further development of safety technology and to make use of the potential of innovative safety-related approaches. The Gesellschaft fuer Anlagen- und Reaktorsicherheit (GRS) mbH, by order of the BMWi, continuously issues information on the status of such investigations by publishing semi-annual and annual progress reports within the series of GRS-F-Fortschrittsberichte (GRS-F-Progress Reports). Each progress report represents a compilation of individual reports about the objectives, work performed, results achieved, next steps of the work etc. The individual reports are prepared in a standard form by the research organisations themselves as documentation of their progress in work and are published by the Research Management Division of GRS within the framework of general information on the progress in reactor safety research. The compilation of the reports is classified according to general topics related to reactor safety research. Further, use is made of the classification system 'Joint Safety Research Index' of the CEC (Commission of the European Communities). The reports are arranged in sequence of their project numbers. It has to be pointed out that the authors of the reports are responsible for the contents of this compilation. The BMWi does not take any responsibility for the correctness, exactness and completeness of the information nor for the observance of private claims of third parties. (orig.)

  1. Resource Conservation and Recovery Act ground-water monitoring projects for Hanford Facilities: Progress report, July 1--September 30, 1989

    International Nuclear Information System (INIS)

    Smith, R.M.; Bates, D.J.; Lundgren, R.E.

    1989-12-01

    This is Volume 1 of a two-volume document that describes the progress of 14 Hanford Site ground-water monitoring projects for the period July 1 to September 30, 1989. This volume discusses the projects; Volume 2 provides as-built diagrams, completion/inspection reports, drilling logs, and geophysical logs for wells drilled, completed, or logged during this period. Volume 2 can be found on microfiche in the back pocket of Volume 1. The work described in this document is conducted by the Pacific Northwest Laboratory under the management of Westinghouse Hanford Company for the US Department of Energy. Concentrations of ground-water constituents are compared to federal drinking water standards throughout this document for reference purposes. All drinking water supplied from the sampled aquifer meets regulatory standards for drinking water quality

  2. Geothermal direct-heat utilization assistance: Federal assistance program. Quarterly project progress report, October--December 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    The report summarizes geothermal technical assistance, R&D and technology transfer activities of the Geo-Heat Center at Oregon Institute of Technology for the first quarter of FY-96. It describes 90 contacts with parties during this period related to technical assistance with geothermal direct heat projects. Areas dealt with include geothermal heat pumps, space heating, greenhouses, aquaculture, equipment and resources. Research activities are summarized on low-temperature resource assessment, geothermal district heating system cost evaluation and silica waste utilization project. Outreach activities include the publication of a geothermal direct use Bulletin, dissemination of information, geothermal library, technical papers and seminars, development of a webpage, and progress monitor reports on geothermal resources and utilization.

  3. Certain amplified genomic-DNA fragments (AGFs) may be involved in cell cycle progression and chloroquine is found to induce the production of cell-cycle-associated AGFs (CAGFs) in Plasmodium falciparum

    OpenAIRE

    Li, Gao-De

    2015-01-01

    It is well known that cyclins are a family of proteins that control cell-cycle progression by activating cyclin-dependent kinase. Based on our experimental results, we propose here a novel hypothesis that certain amplified genomic-DNA fragments (AGFs) may also be required for the cell cycle progression of eukaryotic cells and thus can be named as cell-cycle-associated AGFs (CAGFs). Like fluctuation in cyclin levels during cell cycle progression, these CAGFs are amplified and degraded at diffe...

  4. STYLE - A European Project on Structural Integrity: Progress of the work after 2 Years

    International Nuclear Information System (INIS)

    Heussner, Stefan; Nicak, Tomas; Keim, Elisabeth

    2012-01-01

    The overall objective of STYLE is to assess, optimise and develop the use of advanced tools for the structural integrity assessment of components relevant to ageing and life time management and to support the integration of the knowledge created in the project into main-stream nuclear industry assessment codes.

  5. Nuclear chemistry project. Progress report, January 1, 1978--December 31, 1978

    International Nuclear Information System (INIS)

    Naumann, R.A.

    1978-01-01

    Research on the nuclear chemistry project is summarized including Coulomb capture of negative muons by atoms and molecules, nuclear structure and spectroscopy, and the preparation and use of radioactive targets both to study the internal electric fields acting on the nuclei of foreign atoms introduced in metallic solids by radioactive decay and determination of nuclear moments by optical hyperfine spectroscopy

  6. A visual progression of the Fort Valley Restoration Project treatments using remotely sensed imagery (P-53)

    Science.gov (United States)

    Joseph E. Crouse; Peter Z. Fule

    2008-01-01

    The landscape surrounding the Fort Valley Experimental Forest in northern Arizona has changed dramatically in the past decade due to the Fort Valley Restoration Project, a collaboration between the Greater Flagstaff Forest Partnership, Coconino National Forest, and Rocky Mountain Research Station. Severe wildfires in 1996 sparked community concern to start restoration...

  7. General guidelines for the assessment of internal dose from monitoring data: Progress of the IDEAS project

    International Nuclear Information System (INIS)

    Doerfel, H.; Andrasi, A.; Bailey, M.; Blanchardon, E.; Cruz-Suarez, R.; Berkovski, V.; Castellani, C. M.; Hurtgenv, C.; Leguen, B.; Malatova, I.; Marsh, J.; Stather, J.; Zeger, J.

    2007-01-01

    In recent major international intercomparison exercises on intake and internal dose assessments from monitoring data, the results calculated by different participants varied significantly. Based on this experience the need for harmonisation of the procedures has been formulated within an EU 5. Framework Programme research project. The aim of the project, IDEAS, is to develop general guidelines for standardising assessments of intakes and internal doses. The IDEAS project started in October 2001 and ended in June 2005. The project is closely related to some goals of the work of Committee 2 of the ICRP and since 2003 there has been close cooperation between the two groups. To ensure that the guidelines are applicable to a wide range of practical situations, the first step was to compile a database of well-documented cases of internal contamination. In parallel, an improved version of an existing software package was developed and distributed to the partners for further use. A large number of cases from the database was evaluated independently by the partners and the results reviewed. Based on these evaluations, guidelines were drafted and discussed with dosimetry professionals from around the world by means of a virtual workshop on the Internet early in 2004. The guidelines have been revised and refined on the basis of the experiences and discussions in this virtual workshop. The general philosophy of the Guidelines is presented here, focusing on the principles of harmonisation, optimisation and proportionality. Finally, the proposed Levels of Task to structure the approach of internal dose evaluation are reported. (authors)

  8. Review projects for the US Fusion Program: Progress report, December 1, 1984-February 28, 1987

    International Nuclear Information System (INIS)

    Ribe, F.L.

    1988-01-01

    This paper reviews projects at the University of Washington on the following topics: Magnetic Fusion Energy Program Plan (Feb. 1985); High Density Power Systems; Fusion Systems Studies; Burning Plasmas and Compact Ignition Tokamak; US Magnetic Mirror Program; and Technical Planning Activity (Jan. 1987)

  9. Tree Flora of Sabah and Sarawak projectprogress and future activities

    NARCIS (Netherlands)

    Tan, H.S.; Nadiah, I.; Eryani, S.S.

    2009-01-01

    Tropical forests in Borneo (Brunei Darussalam, Kalimantan, Sabah and Sarawak) are considered as one of the twelve mega biodiversity centres in the world. However, until now, there is no up-to-date or complete documentation on the flora of the island. The Tree Flora of Sabah and Sarawak Project,

  10. Steam Generator Group Project. Progress report on data acquisition/statistical analysis

    International Nuclear Information System (INIS)

    Doctor, P.G.; Buchanan, J.A.; McIntyre, J.M.; Hof, P.J.; Ercanbrack, S.S.

    1984-01-01

    A major task of the Steam Generator Group Project (SGGP) is to establish the reliability of the eddy current inservice inspections of PWR steam generator tubing, by comparing the eddy current data to the actual physical condition of the tubes via destructive analyses. This report describes the plans for the computer systems needed to acquire, store and analyze the diverse data to be collected during the project. The real-time acquisition of the baseline eddy current inspection data will be handled using a specially designed data acquisition computer system based on a Digital Equipment Corporation (DEC) PDP-11/44. The data will be archived in digital form for use after the project is completed. Data base management and statistical analyses will be done on a DEC VAX-11/780. Color graphics will be heavily used to summarize the data and the results of the analyses. The report describes the data that will be taken during the project and the statistical methods that will be used to analyze the data. 7 figures, 2 tables

  11. Standard Terminology for Phenotypic Variations: The Elements of Morphology Project, Its Current Progress, and Future Directions

    NARCIS (Netherlands)

    Carey, John C.; Allanson, Judith E.; Hennekam, Raoul C. M.; Biesecker, Leslie G.

    2012-01-01

    In 2005, the authors of this article formed an international working group to develop standardized definitions and terms to describe the physical variations used in human phenotypic analyses. This project, which came to be known as the Elements of Morphology, resulted in six articles proposing

  12. The Kings River Sustainable Forest Ecosystems Project: inception, objectives, and progress

    Science.gov (United States)

    Jared Verner; Mark T. Smith

    2002-01-01

    The Kings River Sustainable Forest Ecosystems Project, a formal administrative study involving extensive and intensive collaboration between Forest Service managers and researchers, is a response to changes in the agency’s orientation in favor of ecosystem approaches and to recent concern over issues associated with maintenance of late successional forest attributes...

  13. Projecting future air pollution-related mortality under a changing climate: progress, uncertainties and research needs.

    Science.gov (United States)

    Madaniyazi, Lina; Guo, Yuming; Yu, Weiwei; Tong, Shilu

    2015-02-01

    Climate change may affect mortality associated with air pollutants, especially for fine particulate matter (PM2.5) and ozone (O3). Projection studies of such kind involve complicated modelling approaches with uncertainties. We conducted a systematic review of researches and methods for projecting future PM2.5-/O3-related mortality to identify the uncertainties and optimal approaches for handling uncertainty. A literature search was conducted in October 2013, using the electronic databases: PubMed, Scopus, ScienceDirect, ProQuest, and Web of Science. The search was limited to peer-reviewed journal articles published in English from January 1980 to September 2013. Fifteen studies fulfilled the inclusion criteria. Most studies reported that an increase of climate change-induced PM2.5 and O3 may result in an increase in mortality. However, little research has been conducted in developing countries with high emissions and dense populations. Additionally, health effects induced by PM2.5 may dominate compared to those caused by O3, but projection studies of PM2.5-related mortality are fewer than those of O3-related mortality. There is a considerable variation in approaches of scenario-based projection researches, which makes it difficult to compare results. Multiple scenarios, models and downscaling methods have been used to reduce uncertainties. However, few studies have discussed what the main source of uncertainties is and which uncertainty could be most effectively reduced. Projecting air pollution-related mortality requires a systematic consideration of assumptions and uncertainties, which will significantly aid policymakers in efforts to manage potential impacts of PM2.5 and O3 on mortality in the context of climate change. Crown Copyright © 2014. Published by Elsevier Ltd. All rights reserved.

  14. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability

    Directory of Open Access Journals (Sweden)

    Teresa L.F. Ho

    2016-04-01

    Full Text Available Regulation of DNA replication and cell division is essential for tissue growth and maintenance of genomic integrity and is particularly important in tissues that undergo continuous regeneration such as mammary glands. We have previously shown that disruption of the KRAB-domain zinc finger protein Roma/Zfp157 results in hyperproliferation of mammary epithelial cells (MECs during pregnancy. Here, we delineate the mechanism by which Roma engenders this phenotype. Ablation of Roma in MECs leads to unscheduled proliferation, replication stress, DNA damage, and genomic instability. Furthermore, mouse embryonic fibroblasts (MEFs depleted for Roma exhibit downregulation of p21Cip1 and geminin and have accelerated replication fork velocities, which is accompanied by a high rate of mitotic errors and polyploidy. In contrast, overexpression of Roma in MECs halts cell-cycle progression, whereas siRNA-mediated p21Cip1 knockdown ameliorates, in part, this phenotype. Thus, Roma is an essential regulator of the cell cycle and is required to maintain genomic stability.

  15. RPAN: rice pan-genome browser for ∼3000 rice genomes.

    Science.gov (United States)

    Sun, Chen; Hu, Zhiqiang; Zheng, Tianqing; Lu, Kuangchen; Zhao, Yue; Wang, Wensheng; Shi, Jianxin; Wang, Chunchao; Lu, Jinyuan; Zhang, Dabing; Li, Zhikang; Wei, Chaochun

    2017-01-25

    A pan-genome is the union of the gene sets of all the individuals of a clade or a species and it provides a new dimension of genome complexity with the presence/absence variations (PAVs) of genes among these genomes. With the progress of sequencing technologies, pan-genome study is becoming affordable for eukaryotes with large-sized genomes. The Asian cultivated rice, Oryza sativa L., is one of the major food sources for the world and a model organism in plant biology. Recently, the 3000 Rice Genome Project (3K RGP) sequenced more than 3000 rice genomes with a mean sequencing depth of 14.3×, which provided a tremendous resource for rice research. In this paper, we present a genome browser, Rice Pan-genome Browser (RPAN), as a tool to search and visualize the rice pan-genome derived from 3K RGP. RPAN contains a database of the basic information of 3010 rice accessions, including genomic sequences, gene annotations, PAV information and gene expression data of the rice pan-genome. At least 12 000 novel genes absent in the reference genome were included. RPAN also provides multiple search and visualization functions. RPAN can be a rich resource for rice biology and rice breeding. It is available at http://cgm.sjtu.edu.cn/3kricedb/ or http://www.rmbreeding.cn/pan3k. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  16. Extreme project. Progress report 2006; Projet EXTREME. Rapport d'avancement 2006

    Energy Technology Data Exchange (ETDEWEB)

    Eyrolle, F.; Masson, O.; Charmasson, S

    2007-07-01

    The E.X.T.R.E.M.E. project introduced in 2005 to the S.E.S.U.R.E. / L.E.R.C.M. has for objectives to acquire data on the consequences of the extreme climatic meteorological episodes on the distribution of the artificial radioisotopes within the various compartments of the geosphere. This report presents the synthesis of the actions developed in 2006 in positioning and in co financing of the project by means of regional or national research programs (C.A.R.M.A., E.X.T.R.E.M.A., E.C.C.O.R.E.V.I.), of data acquisition, valuation and scientific collaboration. (N.C.)

  17. Explosives disposal demonstration projects. Progress report, April 12, 1995--June 30, 1995

    International Nuclear Information System (INIS)

    Charbeneau, R.

    1995-01-01

    This report contains quarterly reports on two projects. The first is undertaking the environmental restoration at the Pantex Plant. Research objectives are organized under four general tasks: field testing and produced water treatment, bioremediation of contaminated groundwater and soils, vadose zone remediation, and chromium remediation. The other project goal is to demonstrate generation of diamond by explosive compression of Carbon 60 and Carbon 70 and mixtures of these fullerenes. The intent is to exploit expertise developed by Pantex and other DOE Laboratories in the area of understanding and modeling of explosive compression for initiation of nuclear fission reactions to explosively compress carbon in the form of fullerenes with the goal of transforming the material into the diamond phase

  18. Progress of the Enhanced Hanford Single Shell Tank (SST) Integrity Project

    Energy Technology Data Exchange (ETDEWEB)

    Venetz, Theodore J. [Washington River Protection Solutions, Richland, WA (United States); Washenfelder, Dennis J. [Washington River Protection Solutions, Richland, WA (United States); Boomer, Kayle D. [Washington River Protection Solutions, Richland, WA (United States); Johnson, Jeremy M. [USDOE Office of River Protection, Richland, WA (United States); Castleberry, Jim L. [Washington River Protection Solutions, Richland, WA (United States)

    2015-01-07

    To improve the understanding of the single-shell tanks (SSTs) integrity, Washington River Protection Solutions, LLC (WRPS), the USDOE Hanford Site tank contractor, developed an enhanced Single-Shell Tank Integrity Project (SSTIP) in 2009. An expert panel on SST integrity, consisting of various subject matters experts in industry and academia, was created to provide recommendations supporting the development of the project. This panel developed 33 recommendations in four main areas of interest: structural integrity, liner degradation, leak integrity and prevention, and mitigation of contamination migration. In late 2010, seventeen of these recommendations were used to develop the basis for the M-45-10-1 Change Package for the Hanford Federal Agreement and Compliance Order, which is also known as the Tri-Party Agreement.

  19. Recent progress in flow control for practical flows results of the STADYWICO and IMESCON projects

    CERN Document Server

    Barakos, George; Luczak, Marcin

    2017-01-01

    This book explores the outcomes on flow control research activities carried out within the framework of two EU-funded projects focused on training-through-research of Marie Sklodowska-Curie doctoral students. The main goal of the projects described in this monograph is to assess the potential of the passive- and active-flow control methods for reduction of fuel consumption by a helicopter. The research scope encompasses the fields of structural dynamics, fluid flow dynamics, and actuators with control. Research featured in this volume demonstrates an experimental and numerical approach with a strong emphasis on the verification and validation of numerical models. The book is ideal for engineers, students, and researchers interested in the multidisciplinary field of flow control. Provides highly relevant and up-to-date information on the topic of flow control; Includes assessments of a wide range of flow-control technologies and application examples for fixed and rotary-wing configurations; Reinforces reader u...

  20. Statement on current progress of the EPR project. Basic Design phase concluded

    International Nuclear Information System (INIS)

    Lamprecht, F.

    1997-01-01

    Against the background of expected increasing demand for electric power worldwide it is obvious that any existing technology for electricity generation will be required in the future to meet the demand, just as well as technology improvements. Nuclear industry's answer to this challenge are activities for further evolution of the PWR concept as a joint venture of France and Germany for development of the European Pressurized Water Reactor (EPR). The project participants are reactor manufacturers and operators of both countries as well as organisations responsible for reactor safety. The Basic Design phase which started in 1995, after prior definition of the common safety philosophy, has recently been concluded. The results have been presented at a meeting in October 1997 in Cologne, organised by KTG and SFEN, the countries' respective nuclear societies. The broad statement is that project work was successful in achieving two major objectives: A well-balanced safety concept, and a competitive design. (orig./CB) [de

  1. Progress Report

    DEFF Research Database (Denmark)

    Duer, Karsten

    1999-01-01

    Progress report describing the work carried out by the Danish participant in the ALTSET project in the period January 1999 to July 1999.......Progress report describing the work carried out by the Danish participant in the ALTSET project in the period January 1999 to July 1999....

  2. Final Progress Report for Project Entitled: Quantum Dot Tracers for Use in Engineered Geothermal Systems

    Energy Technology Data Exchange (ETDEWEB)

    Rose, Peter [Univ. of Utah, Salt Lake City, UT (United States); Bartl, Michael [Univ. of Utah, Salt Lake City, UT (United States); Reimus, Paul [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Williams, Mark [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Mella, Mike [Univ. of Utah, Salt Lake City, UT (United States)

    2015-09-12

    The objective of this project was to develop and demonstrate a new class of tracers that offer great promise for use in characterizing fracture networks in EGS reservoirs. From laboratory synthesis and testing through numerical modeling and field demonstrations, we have demonstrated the amazing versatility and applicability of quantum dot tracers. This report summarizes the results of four years of research into the design, synthesis, and characterization of semiconductor nanocrystals (quantum dots) for use as geothermal tracers.

  3. General-purpose heat source project and space nuclear safety and fuels program. Progress report

    International Nuclear Information System (INIS)

    Maraman, W.J.

    1979-12-01

    This formal monthly report covers the studies related to the use of 238 PuO 2 in radioisotopic power systems carried out for the Advanced Nuclear Systems and Projects Division of the Los Alamos Scientific Laboratory. The two programs involved are general-purpose heat source development and space nuclear safety and fuels. Most of the studies discussed hear are of a continuing nature. Results and conclusions described may change as the work continues

  4. Observational studies in South African mines to mitigate seismic risks: a mid-project progress report

    CSIR Research Space (South Africa)

    Durrheim, RJ

    2013-10-01

    Full Text Available such as Japan. A 5-year collaborative project entitled "Observational studies in South African mines to mitigate seismic risks" was launched in 2010 to address these risks, drawing on over a century of South African and Japanese research experience... network in the mining districts. Figure 1. Schematic illustration of the research design. Jpn - Japanese researchers; CSIR - Council for Scientific and Industrial Research; CGS - Council for Geoscience The knowledge gained during the course...

  5. The Agricultural Model Intercomparison and Improvement Project (AgMIP): Overview and Progress

    Science.gov (United States)

    Rosenzweig, C.; Hatfield, J.; Jones, J. W.; Ruane, A. C.

    2012-12-01

    The Agricultural Model Intercomparison and Improvement Project (AgMIP) is an international effort to assess the state of global agricultural modeling and to understand climate impacts on the agricultural sector. AgMIP connects the climate science, crop modeling, and agricultural economic modeling communities to generate probabilistic projections of current and future climate impacts. The goals of AgMIP are to improve substantially the characterization of risk of hunger and world food security due to climate change and to enhance adaptation capacity in both developing and developed countries. This presentation will describe the general approach of AgMIP and highlight its findings and activities. AgMIP crop model intercomparisons have been established for wheat (27 models participating), maize (25 models), and rice (15+ models), and are being established for sugarcane, soybean, sorghum/millet, and peanut. In coordination with these pilots, methodologies to utilize weather generators and downscaled climate simulations for agricultural applications are under development. An AgMIP global agricultural economics model intercomparison with participation of 11 international groups is ongoing, and a number of global biophysical models are currently being evaluated for future climate impacts on agricultural lands both as part of the Inter-Sectoral Impact Model Intercomparison Project (ISI-MIP) and for contribution to the IPCC Fifth Assessment Report (AR5). AgMIP is also organizing regional research efforts, and has already held workshops in South America, Sub-Saharan Africa, South Asia, Europe, and North America. Outcomes from these meetings have informed AgMIP activities, and 10 research teams from Sub-Saharan Africa and South Asia have been selected for project funding. Additional activities are planned for Australia and East Asia. As the AgMIP research community continues to work towards its goals, three key cross-cutting scientific challenges have emerged and are being

  6. Evaluation and monitoring of wild/natural steelhead trout production: project progress report, 1996; ANNUAL

    International Nuclear Information System (INIS)

    Leth, Brian D.; Holubetz, Terry; Nemeth, Doug

    2000-01-01

    This project was initiated to provide additional, and more definitive, information regarding wild steelhead Oncorhynchus mykiss populations in Idaho. Important streams for wild steelhead production were identified and selected for monitoring. Monitoring activities employed among streams varied, but generally included: aerial redd counts, placement of adult weirs, enumeration of juveniles through mask and snorkel counts, and emigrant trapping. This report details activities during the 1996 field season

  7. Monitoring the progress of NFFO-3 projects: short rotation willow coppice - agronomy and economics

    International Nuclear Information System (INIS)

    Twemlow, R.K.M.

    1997-01-01

    The involvement of Sidney C. Banks p.l.c., and the subsidiary company Banks Agriculture, in fuel supply for biofuelled power stations started some 4 years ago. When identifying possible opportunities and crops it was apparent that, with the third tranche of Non-Fossil Fuel Obligations (NFFO3) coming up, there were companies looking at developing biofuelled power stations, using Short Rotation Coppice (SRC) and for this they needed to link with potential fuel suppliers. Sidney C. Banks p.l.c. linked with South Western Power (SWP) (a subsidiary of South Western Electricity p.l.c. (SWEB)) to act as their sole fuel supplier on projected biofuel wood power stations. Subsequently two wood fuelled power stations were awarded to SWP and Sidney C. Banks p.l.c. in the 3rd Tranche from NFFO, in December 1994. The objectives of this project were to: establish a network of SRC growers to produce fuel for a NFFO project; monitor the success of the enterprise; implement a programme of constant improvement to optimise the fuel supply strategy; and provide valuable information as to the performance of the SRC crop in large scale commercial production. (author)

  8. Progress on the Femto-Slicing Project at the Synchrotron SOLEIL

    International Nuclear Information System (INIS)

    Prigent, P; Hollander, Ph; Labat, M; Couprie, M E; Marlats, J L; Laulhé, C; Luning, J; Moreno, T; Morin, P; Nadji, A; Polack, F; Ravy, S; Silly, M; Sirotti, F

    2013-01-01

    The aim of the Femto-Slicing project at SOLEIL is to generate 100 fs X-rays pulses on two beamlines, CRISTAL and TEMPO, for pump-probe experiments in the hard and soft X-rays regions. Two fs lasers are currently in operation on TEMPO and CRISTAL for pump-probe experiments on the ps time scale enabling time resolved photoemission and photodiffraction studies. The Femto-Slicing project is based on the fs laser of the CRISTAL beamline, which can be adjusted to deliver 3 mJ pulses of 30 fs duration at 2.5 kHz. The laser beam will be separated in three branches: one delivering about 2 mJ to the modulator Wiggler and the other ones delivering the remaining energy to the TEMPO and CRISTAL experiments. This layout will yield natural synchronization between IR laser pump and X-ray probe pulses, only affected by jitter associated with beam transport. In this paper, we present the current status of the Femto-Slicing project at SOLEIL, with particular emphasis on the expected performance, and the design and construction of the laser beam transport and the diagnostics implementation.

  9. Progress report on decommissioning activities at the Fernald Environmental Management Project (FEMP) site

    International Nuclear Information System (INIS)

    1998-01-01

    The Fernald Environmental Management Project (FEMP), is located about 18 miles northwest of Cincinnati, Ohio. Between 1953 and 1989, the facility, then called the Feed Material Production Center or FMPC, produced uranium metal products used in the eventual production of weapons grade material for use by other US Department of Energy (DOE) sites. In 1989, FMPC's production was suspended by the federal government in order to focus resources on environmental restoration versus defense production. In 1992, Fluor Daniel Fernald assumed responsibility for managing all cleanup activities at the FEMP under contract to the DOE. In 1990, as part of the remediation effort, the site was divided into five operable units based on physical proximity of contaminated areas, similar amounts of types of contamination, or the potential for a similar technology to be used in cleanup activities. This report continues the outline of the decontamination and decommissioning (D and D) activities at the FEMP site Operable Unit 3 (OU3) and provides an update on the status of the decommissioning activities. OU3, the Facilities Closure and Demolition Project, involves the remediation of more than 200 uranium processing facilities. The mission of the project is to remove nuclear materials stored in these buildings, then perform the clean out of the buildings and equipment, and decontaminate and dismantle the facilities

  10. UK Safeguards R and D Project progress report for the period May 1987 -April 1988

    International Nuclear Information System (INIS)

    Packer, T.W.

    1988-09-01

    The main categories of task included in the United Kingdom safeguards research and development programme are summarised in the first section. These are: tasks concerned with the development of instruments and techniques for reprocessing and centrifuge enrichment plant safeguards; tasks concerned with the development of instruments and techniques for general application in the field of safeguards; tasks which are services to the International Atomic Energy Authority and exploratory and short term tasks which occur from time to time. The next three sections contain progress reports on the individual tasks and section 6 lists reports and papers relevant to work on UK safeguards research and development published between May 1987 and April 1988. (U.K.)

  11. The Walla Walla Basin Natural Production Monitoring and Evaluation Project : Progress Report, 1999-2002.

    Energy Technology Data Exchange (ETDEWEB)

    Contor, Craig R.; Sexton, Amy D.

    2003-06-02

    The Walla Walla Basin Natural Production Monitoring and Evaluation Project (WWNPME) was funded by Bonneville Power Administration (BPA) as directed by section 4(h) of the Pacific Northwest Electric Power Planning and Conservation Act of 1980 (P. L. 96-501). This project is in accordance with and pursuant to measures 4.2A, 4.3C.1, 7.1A.2, 7.1C.3, 7.1C.4 and 7.1D.2 of the Northwest Power Planning Council's (NPPC) Columbia River Basin Fish and Wildlife Program (NPPC 1994). Work was conducted by the Fisheries Program of the Confederated Tribes of the Umatilla Indian Reservation (CTUIR) under the Walla Walla Basin Natural Production Monitoring and Evaluation Project (WWNPME). Chapter One provides an overview of the entire report and how the objectives of each statement of work from 1999, 2000, 2001, and 2002 contract years are organized and reported. Chapter One also provides background information relevant to the aquatic resources of the Walla Walla River Basin. Objectives are outlined below for the statements of work for the 1999, 2000, 2001 and 2002 contract years. The same objectives were sometimes given different numbers in different years. Because this document is a synthesis of four years of reporting, we gave objectives letter designations and listed the objective number associated with the statement of work for each year. Some objectives were in all four work statements, while other objectives were in only one or two work statements. Each objective is discussed in a chapter. The chapter that reports activities and findings of each objective are listed with the objective below. Because data is often interrelated, aspects of some findings may be reported or discussed in more than one chapter. Specifics related to tasks, approaches, methods, results and discussion are addressed in the individual chapters.

  12. The Progress of the HIRFL-CSR Project and the Commissioning of the Cluster Target

    International Nuclear Information System (INIS)

    Cai Xiaohong; Lu Rongchun; Shao Caojie; Ruan, Fangfang; Yu, Deyang; Li Mingsheng; Torpokov, D K; Shestakov, Yu V; Sadykov, Rs; Zevakov, S A; Xia Jiawen; Zhan Wenlong

    2007-01-01

    HIRFL-CSR, a new cooler-storage-ring project, is the post-acceleration system of the Heavy Ion Research Facility in Lanzhou (HIRFL) consisting of a main ring (CSRm) and an experimental ring (CSRe). The construction of the HIRFL-CSR complex is nearing completion. The stored beam in the CSRm has been observed. Recently, the stored beam was accelerated from 7 MeV/u to 30 MeV/u. The cluster target is located in one straight section of the CSRe providing cluster jet targets of inert gases and small molecular gases. The cluster target has been finished and the results of the test running are presented

  13. Bill project introducing a progressive price fixing of energy - Nr 150

    International Nuclear Information System (INIS)

    Brottes, Francois; Le Roux, Bruno

    2012-01-01

    This bill project aims at speeding up energy transition by inciting households to reduce their consumption, and at accompanying the unavoidable increase of energy prices. It introduces a rather complex process to establish the energy tariff, and notably a bonus-malus principle. The objective is to incite households to better insulate their main housing. It also takes social tariffs into account, defines institutions and representative for energy regulation, proposes the implementation of a public service for housing energy performance, and addresses the issue of energy supply in winter to households in precarious situation

  14. Blind test results - measure of incessant progress in personnel monitoring services at Kudankulam Nuclear Power Project

    International Nuclear Information System (INIS)

    Jashi, K.B.; Ashok Kumar, A.; Pandaram, P.

    2018-01-01

    Personnel Monitoring Services (PMS) at Thermoluminescent Dosimeter (TLD) laboratory, Kudankulam Nuclear Power Project (KKNPP) involve monitoring of about 1500 radiation workers of KKNPP for external radiation exposure on monthly basis. Quality assurance in PMS provides an overall estimate of accuracy which depends not only upon the dosimeter quality and reader performance but also on data interpretation, applicable software and the system. Therefore it measures the effectiveness of quality control mechanism. Blind test is an effective tool to check the performance of the processor without their knowledge during routine processing of the service cards

  15. General-purpose heat source project and space nuclear safety fuels program. Progress report, February 1980

    International Nuclear Information System (INIS)

    Maraman, W.J.

    1980-05-01

    This formal monthly report covers the studies related to the use of 238 PuO 2 in radioisotopic power systems carried out for the Advanced Nuclear Systems and Projects Division of the Los Alamos Scientific Laboratory. The two programs involved are: General-Purpose Heat Source Development and Space Nuclear Safety and Fuels. Most of the studies discussed here are of a continuing nature. Results and conclusions described may change as the work continues. Published reference to the results cited in this report should not be made without the explicit permission of the person in charge of the work

  16. Retrieval process development and enhancements project Fiscal year 1995: Simulant development technology task progress report

    International Nuclear Information System (INIS)

    Golcar, G.R.; Bontha, J.R.; Darab, J.G.

    1997-01-01

    The mission of the Retrieval Process Development and Enhancements (RPD ampersand E) project is to develop an understanding of retrieval processes, including emerging and existing technologies, gather data on these technologies, and relate the data to specific tank problems such that end-users have the requisite technical bases to make retrieval and closure decisions. The development of waste simulants is an integral part of this effort. The work of the RPD ampersand E simulant-development task is described in this document. The key FY95 accomplishments of the RPD ampersand E simulant-development task are summarized below

  17. Human genetics and genomics a decade after the release of the draft sequence of the human genome

    Science.gov (United States)

    2011-01-01

    Substantial progress has been made in human genetics and genomics research over the past ten years since the publication of the draft sequence of the human genome in 2001. Findings emanating directly from the Human Genome Project, together with those from follow-on studies, have had an enormous impact on our understanding of the architecture and function of the human genome. Major developments have been made in cataloguing genetic variation, the International HapMap Project, and with respect to advances in genotyping technologies. These developments are vital for the emergence of genome-wide association studies in the investigation of complex diseases and traits. In parallel, the advent of high-throughput sequencing technologies has ushered in the 'personal genome sequencing' era for both normal and cancer genomes, and made possible large-scale genome sequencing studies such as the 1000 Genomes Project and the International Cancer Genome Consortium. The high-throughput sequencing and sequence-capture technologies are also providing new opportunities to study Mendelian disorders through exome sequencing and whole-genome sequencing. This paper reviews these major developments in human genetics and genomics over the past decade. PMID:22155605

  18. Patterns of cross-contamination in a multispecies population genomic project: detection, quantification, impact, and solutions.

    Science.gov (United States)

    Ballenghien, Marion; Faivre, Nicolas; Galtier, Nicolas

    2017-03-29

    Contamination is a well-known but often neglected problem in molecular biology. Here, we investigated the prevalence of cross-contamination among 446 samples from 116 distinct species of animals, which were processed in the same laboratory and subjected to subcontracted transcriptome sequencing. Using cytochrome oxidase 1 as a barcode, we identified a minimum of 782 events of between-species contamination, with approximately 80% of our samples being affected. An analysis of laboratory metadata revealed a strong effect of the sequencing center: nearly all the detected events of between-species contamination involved species that were sent the same day to the same company. We introduce new methods to address the amount of within-species, between-individual contamination, and to correct for this problem when calling genotypes from base read counts. We report evidence for pervasive within-species contamination in this data set, and show that classical population genomic statistics, such as synonymous diversity, the ratio of non-synonymous to synonymous diversity, inbreeding coefficient F IT , and Tajima's D, are sensitive to this problem to various extents. Control analyses suggest that our published results are probably robust to the problem of contamination. Recommendations on how to prevent or avoid contamination in large-scale population genomics/molecular ecology are provided based on this analysis.

  19. History and progress of the North American Soil Geochemical Landscapes Project, 2001-2010

    Science.gov (United States)

    Smith, David B.; Cannon, William F.; Woodruff, Laurel G.; Rivera, Francisco Moreira; Rencz, Andrew N.; Garrett, Robert G.

    2012-01-01

    In 2007, the U.S. Geological Survey, the Geological Survey of Canada, and the Mexican Geological Survey initiated a low-density (1 site per 1600 km2, 13323 sites) geochemical and mineralogical survey of North American soils (North American Soil Geochemical Landscapes Project). Sampling and analytical protocols were developed at a series of workshops in 20032004 and pilot studies were conducted from 20042007. The ideal sampling protocol at each site includes a sample from 05 cm depth, a composite of the soil A horizon, and a sample from the soil C horizon. The 3, HClO4, and HF. Separate methods are used for As, Hg, Se, and total C on this same size fraction. The major mineralogical components are determined by a quantitative X-ray diffraction method. Sampling in the conterminous U.S. was completed in 2010 (c. 4800 sites) with chemical and mineralogical analysis currently underway. In Mexico, approximately 66% of the sampling (871 sites) had been done by the end of 2010 with completion expected in 2012. After completing sampling in the Maritime provinces and portions of other provinces (472 sites, 7.6% of the total), Canada withdrew from the project in 2010. Preliminary results for a swath from the central U.S. to Florida clearly show the effects of soil parent material and climate on the chemical and mineralogical composition of soils. A sample archive will be established and made available for future investigations.

  20. Synchrotron topography project. Progress report, January 20, 1982-October 20, 1982

    International Nuclear Information System (INIS)

    Bilello, J.C.; Chen, H.; Hmelo, A.B.; Liu, J.M.; Birnbaum, H.K.; Herley, P.J.; Green, R.E. Jr.

    1982-01-01

    The collaborators have participated in the Synchrotron Topography Project (STP) which has designed and developed instrumentation for an x-ray topography station at the National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory (BNL). The two principle instruments constructed consist of a White Beam Camera (WBC) and a Multiple Crystal Camera (MCC) with high planar collimation and wide area image coverage. It is possible to perform in-situ studies in a versatile environmental chamber equipped with a miniature mechanical testing stage for both the WBC and MCC systems. Real-time video imaging plus a rapid feed cassette holder for high resolution photographic plates are available for recording topographs. Provisions are made for other types of photon detection as well as spectroscopy. The facilities for the entire station have been designed for remote operation using a LSI-11/23 plus suitable interfacing. These instruments will be described briefly and the current status of the program will be reviewed. The Appendix of this report presents titles, authors and abstracts of other technical work associated with this project during the current period

  1. Mexico City air quality: Progress of an international collaborative project to define air quality management options

    International Nuclear Information System (INIS)

    Streit, G.E.

    1992-01-01

    Mexico City, faces a severe air pollution problem due to a combination of circumstances. The city is in a high mountain basin at a subtropical latitude. The basin setting inhibits dispersion of pollution and contributes to frequent wintertime thermal inversions which further trap pollutants near the surface. The elevation and latitude combine to provide plentiful sunshine which, in comparison to more northern latitudes, is enhanced in the UV radiation which drives atmospheric photochemistry to produce secondary pollutants such as ozone. The Area Metropolitana de la Ciudad de Mexico AMCW is defined to include the 16 delegations of the Federal District (D.F.) and 17 highly urbanized municipalities in the State of Mexico which border the D.F. The 1990 census (XI Censo General de Poblacion y Vivienda de 1990) records that slightly over 15 million people live in the AMCM. There are numerous other nearby communities which are in the airshed region of Mexico City, but which are not included in the definition and population of the AMCM. The Mexico City Air Quality Research Initiative is one project that is examining the complex relationship between air pollution, economic growth, societal values, and air quality management policies. The project utilizes a systems approach including computer modeling, comprehensive measurement studies of Mexico City's air pollutants, environmental chemical reaction studies and socioeconomic analysis. Los Alamos National Laboratory (USA) and the Mexican Petroleum Institute are the designated lead institutions

  2. UP2 400 High Activity Oxide Legacy Waste Retrieval Project Scope and Progress-13048

    Energy Technology Data Exchange (ETDEWEB)

    Chabeuf, Jean-Michel; Varet, Thierry [AREVA Site Value Development Business Unit, La Hague Site (France)

    2013-07-01

    The High Activity Oxide facility (HAO) reprocessed sheared and dissolved 4500 metric tons of light water reactor fuel the fuel of the emerging light water reactor spent fuel between 1976 and 1998. Over the period, approximately 2200 tons of process waste, composed primarily of sheared hulls, was produced and stored in a vast silo in the first place, and in canisters stored in pools in subsequent years. Upon shutdown of the facility, AREVA D and D Division in La Hague launched a thorough investigation and characterization of the silos and pools content, which then served as input data for the definition of a legacy waste retrieval and reconditioning program. Basic design was conducted between 2005 and 2007, and was followed by an optimization phase which lead to the definition of a final scenario and budget, 12% under the initial estimates. The scenario planned for the construction of a retrieval and reconditioning cell to be built on top of the storage silo. The retrieved waste would then be rinsed and sorted, so that hulls could subsequently be sent to La Hague high activity compacting facility, while resins and sludge would be cemented within the retrieval cell. Detailed design was conducted successfully from 2008 until 2011, while a thorough research and development program was conducted in order to qualify each stage of the retrieval and reconditioning process, and assist in the elaboration of the final waste package specification. This R and D program was defined and conducted as a response and mitigation of the major project risks identified during the basic design process. Procurement and site preparatory works were then launched in 2011. By the end of 2012, R and D is nearly completed, the retrieval and reconditioning process have been secured, the final waste package specification is being completed, the first equipment for the retrieval cell is being delivered on site, while preparation works are allowing to free up space above and around the silo, to

  3. Captive Rearing Program for Salmon River Chinook Salmon : Project Progress Report, 2001 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Venditti, David A.

    2003-10-01

    distributed themselves throughout the study section and displayed a progression of habitat associations and behavior consistent with progressing maturation and the onset of spawning. Five of the 18 redds spawned by captive-reared parents were hydraulically sampled to assess survival to the eyed stage of development. Eyed-eggs were collected from four of these, and survival to this stage ranged from 0%-89%. Expanding these results to the remaining redds produced an estimate of 15,000 eyed-eggs being produced by captive-reared fish.

  4. Overview of the Last Progresses for the European Test Blanket Modules Projects

    International Nuclear Information System (INIS)

    Salavy, J.-F.; Rampal, G.; Boccaccini, L.V.; Meyder, R.; Neuberger, H.; Laesser, R.; Poitevin, Y.; Zmitko, M.; Rigal, E.

    2006-01-01

    The long-term objective of the EU Breeding Blankets programme is the development of DEMO breeding blankets, which shall assure tritium self-sufficiency, an economically attractive use of the heat produced inside the blankets for electricity generation and a sufficiently high shielding of the superconducting magnets for long time operation. In the short-term so-called DEMO relevant Test Blanket Modules (TBMs) of these breeder blanket concepts shall be designed, manufactured, tested, installed, commissioned and operated in ITER for first tests in a fusion environment. The Helium Cooled Lithium-Lead (HCLL) breeder blanket and the Helium Cooled Pebble Bed (HCPB) concepts are the two breeder blanket lines presently developed by the EU. The main objective of the EU test strategy related to TBMs in ITER is to provide the necessary information for the design and fabrication of breeding blankets for a future DEMO reactor. EU TBMs shall therefore use the same structural and functional materials, apply similar fabrication technologies, and test adequate processes and components. This paper gives an overview of the last progresses in terms of system design, calculations, test program, safety and R-and-D done these last two years in order to cope with the ambitious objective to introduce two EU TBM systems for day-1 of ITER operation. The engineering design of the two systems is mostly concluded and the priority is now on the development and qualification of the fabrication technologies. From calculations point of view, the last modelling efforts related to the thermal-hydraulic of the first wall, the tritium behaviour, and the box thermal and mechanical resistance in accidental conditions are presented. Last features of the TBM and cooling system designs and integration in ITER reactor are highlighted. In particular, this paper also describes the safety and licensing analyses performed for each concept to be able to include the TBM systems in the ITER preliminary safety report

  5. Captive Rearing Program for Salmon River Chinook Salmon, 2000 Project Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Venditti, David A.

    2002-04-01

    During 2000, the Idaho Department of Fish and Game (IDFG) continued to develop techniques to rear chinook salmon Oncorhynchus tshawytscha to sexual maturity in captivity and to monitor their reproductive performance under natural conditions. Eyed-eggs were collected to establish captive cohorts from three study streams and included 503 eyed-eggs from East Fork Salmon River (EFSR), 250 from the Yankee Fork Salmon River, and 304 from the West Fork Yankee Fork Salmon River (WFYF). After collection, the eyed-eggs were immediately transferred to the Eagle Fish Hatchery, where they were incubated and reared by family group. Juveniles collected the previous summer were PIT and elastomer tagged and vaccinated against vibrio Vibrio spp. and bacterial kidney disease before the majority (approximately 75%) were transferred to the National Marine Fisheries Service, Manchester Marine Experimental Station for saltwater rearing through sexual maturity. Smolt transfers included 158 individuals from the Lemhi River (LEM), 193 from the WFYF, and 372 from the EFSR. Maturing fish transfers from the Manchester facility to the Eagle Fish Hatchery included 77 individuals from the LEM, 45 from the WFYF, and 11 from the EFSR. Two mature females from the WFYF were spawned in captivity with four males in 2000. Only one of the females produced viable eggs (N = 1,266), which were placed in in-stream incubators by personnel from the Shoshone-Bannock Tribe. Mature adults (N = 70) from the Lemhi River were released into Big Springs Creek to evaluate their reproductive performance. After release, fish distributed themselves throughout the study section and displayed a progression of habitat associations and behavior consistent with progressing maturation and the onset of spawning. Fifteen of the 17 suspected redds spawned by captive-reared parents in Big Springs Creek were hydraulically sampled to assess survival to the eyed stage of development. Eyed-eggs were collected from 13 of these, and

  6. Progress report of the S.E.N.S.I.B. project 2005-2008

    International Nuclear Information System (INIS)

    Baumont, G.; Bourcier, L.; Briand, B.; Chojnacki, E.; Dufeu, A.; Duffa, C.; Durand, V.; Eyrolle, F.; Larue, C.; Levain, A.; Masson, O.; Mercat, C.; Metivier, J.M.; Pey, B.; Pourcelot, L.; Renaud, Ph.; Roussel-Debet, S.; Thebault, H.

    2008-01-01

    The aim of the project is to define a method for classifying territories by their sensitivity to a radioactive pollution, on the basis of their environmental and social characteristics, i.e. those characteristics associated with the human use of the environment. For example, the sensitivity of an urban environment will depend on the population density, the area covered, the urban services and their response to the contamination. The sensitivity of an agricultural area will be determined by the food chain and the way in which it is affected by contamination of the soil, crops or livestock. The end result of the S.E.N.S.I.B. project will be a standardised tool capable of representing and comparing the sensitivity of an area to radioactive pollution using a single measurement scale. This standardized measurement of the environmental and population characteristics will be useful in risk assessment and management, and in all the operational stages of a nuclear installation including commissioning, normal operation, accident and post-accident situations, and during decommissioning. The project will provide mappings of the sensitivity to a range of concrete scenarios, and of the vulnerability of each area once the probability of pollution occurring has been taken into account. The concept of sensitivity is shared by other fields besides radioecology and can be used to assess vulnerability to chemical pollution, climate change, or population pressures. The results obtained from the S.E.N.S.I.B. project will be applicable to other fields, especially that of chemical pollution, though a range of collaborations and partnerships. The S.E.N.S.I.B. project is divided into five stages. These are: Identification of sensitivity factors, i.e. all the characteristics of a territory likely to make the environment sensitive to a given type of pollution. Characterization of their ranges of values. Conversion of each range of values into a range of scores. Allocation of a weighting to be

  7. An information and dialogue conference on the human genome project (HGP) for the minority communities in the state of Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-06-01

    Zeta Phi Beta Sorority National Educational Foundation, in cooperation with Xavier University of New Orleans, and the New Orleans District Office of the United States Equal Employment Opportunity Commission, held the Information and Dialogue Conference on the Human Genome Project for the Minority Communities in the State of Louisiana on April 16-17, 1999. The Conference was held on the campus of Xavier University in New Orleans. Community leaders, government officials, minority professional and social organizations leaders, religious leaders, persons from the educational and academic community, and students were invited. Conference objectives included bringing HGP information and a focus in the minority community on the project, in clear and understandable terms, to spread the work in the minority community about the project; to explore the likely positive implications with respect to health care and related matters; to explore possible negative results and strategies to meet them; to discuss the social, legal, and ethical implications; and to facilitate minority input into the HGP as it develops.

  8. Recent progress in volcanism studies: Site characterization activities for the Yucca Mountain site characterization project

    International Nuclear Information System (INIS)

    Crowe, B.M.; Valentine, G.; Morley, R.; Perry, F.V.

    1992-01-01

    Significant progress has been made on volcanism studies over the past calendar year. There are a number of major highlights from this work. Geochronology data have been obtained for the Lathrop Wells center using a range of isotopic, radiogenic, and age-calibrated methods. Initial work is encouraging but still insufficient to resolve the age of the center with confidence. Geologic mapping of the Sleeping Butte volcanic centers was completed and a report issued on the geology and chronology data. Twenty shallow trenches have been constructed in volcanic units of the Lathrop Wells volcanic center. Results of detailed studies of the trenches support a polycyclic eruptive history. New soil data from the trenches continue to support a late Pleistocene or Holocene age for many of the volcanic units at the center. Geochemical data (trace element and isotopic analysis) show that the volcanic units of the Lathrop Wells center cannot be related to one another by fractional crystallization of a single magma batch, supporting a polycyclic model of volcanism. Structural models using existing data are used to evaluate the probability of magmatic disruption of a potential repository. Several permissive models have been developed but none lead to significant differences in calculating the disruption ratio. Work was initiated on the eruptive and subsurface effects of magmatic activity on a repository. (author)

  9. Progress on the superconducting magnet for the time projection chamber experiment (TPC) at PEP

    International Nuclear Information System (INIS)

    Green, M.A.; Eberhard, P.H.; Burns, W.A.

    1980-01-01

    The TPC (Time Projection Chamber) experiment at PEP will have a two meter inside diameter superconducting magnet which creatests a 1.5 T uniform solenoidal field for the TPC. The superconducting magnet coil, cryostat, cooling system, and the TPC gas pressure vessel (which operatests at 11 atm) were designed to be about two thirds of a radiation length thick. As a result, a high current density coil design was chosen. The magnet is cooled by forced flow two phase helium. The TPC magnet is the largest adiabatically stable superconducting magnet built to date. The paper presents the parameters of the TPC thin solenoid and its subsystems. Tests results from the Spring 1980 cryogenic tes are presented. The topics to be dealt with in the paper are cryogenic services and the tests of magnet subsystems such as the folded current leads. Large thin superconducting magnet technology will be important to large detectors to be used on LEP

  10. Rhode Island crystalline repository siting project: Technical progress report, Calendar year 1986

    International Nuclear Information System (INIS)

    Vild, B.

    1987-01-01

    A Nuclear Waste Fund established by the Nuclear Waste Policy Act of 1982 provides financial support to affected states to participate in the high-level radioactive waste repository siting program of the US Department of Energy. In Rhode Island, that function was performed by a multidisciplinary Project Review Team consisting of staff from three State agencies. Members of the Review Team attended several meetings in 1986 to discuss mutual concerns with Federal, State and Tribal officials. Comments were developed on DOE's Draft Area Recommendation Report. Members of the Review Team also testified at a public hearing in Providence on the Draft ARR, and developed and distributed a public information booklet. In May, Secretary of Energy John Herrington announced the suspension of the crystalline repository siting program. The remainder of the year was spent monitoring litigation challenging that decision and pending legislation. Administrative phase-down of the program was essentially complete by the close of the calendar year

  11. Technical progress report: Rhode Island crystalline repository project, calendar year 1985

    International Nuclear Information System (INIS)

    Vild, B.F.

    1985-01-01

    A Nuclear Waste Fund established by the Nuclear Waste Policy Act of 1982 provides financial support to affected states to participate in the high-level radioactive waste repository siting program of the US Department of Energy. In Rhode Island, that function is performed by a multidisciplinary Project Review Team consisting of staff from three State agencies. Members of the Review Team attended several meetings in 1985 to voice their concerns directly to DOE. Written comments were also submitted on draft plans and reports. Among the issues raised were inconsistencies in the geologic and environmental data used to screen potential repository sites, the role of Monitored Retrievable Storage (MRS) in the repository program, and regulations regarding the transportation and storage of nuclear waste. The Review Team also began work on a public information booklet describing the repository program in nontechnical terms. That booklet will be distributed widely upon completion

  12. Rhode Island crystalline repository project: Technical progress report, 1984-1986

    International Nuclear Information System (INIS)

    1986-01-01

    A Nuclear Waste Fund established by the Nuclear Waste Policy Act of 1982 provided financial support to the State of Rhode Island for its participation in the high-level radioactive waste repository siting program. In 1984, the Office of the Governor set up a multidisciplinary Project Review Team consisting of staff from three State agencies and the University of Rhode Island. Members of the Review Team attended several meetings throughout the reporting period to voice their concerns about siting directly to the US Department of Energy (DOE). Written comments were also submitted on draft plans and reports. Many of Rhode Island's recommendations in these comments were later adopted. In May, 1986, Secretary of Energy John Herrington announced the suspension of the crystalline repository siting program. The remainder of the year was spent monitoring litigation challenging that decision and pending legislation. Administrative phase-down of the program was essentially complete by the close of the calendar year

  13. Progress report on research and development work 1983 of the nuclear fusion project

    International Nuclear Information System (INIS)

    Finken, D.

    1984-02-01

    The studies of the Kernforschungszentrum Karlsruhe on fusion using magnetic confinement are included in the nuclear fusion project and embedded into the European Fusion Technology Programme. The studies are promoted via an association contract between KfK and Euratom by the European Commission. Some of the studies exceed the volume defined in the technology programme. These contributions, most of them studies, help to connect the various sectors and prepare new tasks, with regard to the extension of the technology programme to be expected in the coming years and the planning activities for NET. The reports summarized here are contributions of the institutes and thus arranged according to organization units. The tasks which the KfK has taken over from the Fusion Technology Programme of the EC are compiled in the appendix. (orig./GG) [de

  14. Progress Report on the GROWTH (GNSS Reflectometry for Ocean Waves, Tides, and Height) Research Project

    Science.gov (United States)

    Kitazawa, Y.; Ichikawa, K.; Akiyama, H.; Ebinuma, T.; Isoguchi, O.; Kimura, N.; Konda, M.; Kouguchi, N.; Tamura, H.; Tomita, H.; Yoshikawa, Y.; Waseda, T.

    2016-12-01

    Global Navigation Satellite Systems (GNSS), such as GPS is a system of satellites that provide autonomous geo-spatial positioning with global coverage. It allows small electronic receivers to determine their location to high precision using radio signals transmitted from satellites, GNSS reflectometry (GNSS-R) involves making measurements from the reflections from the Earth of navigation signals from GNSS satellites. Reflected signals from sea surface are considered that those are useful to observe sea state and sea surface height. We have started a research program for GNSS-R applications on oceanographic observations under the contract with MEXT (Ministry of Education Culture, Sports, Science and Technology, JAPAN) and launched a Japanese research consortium, GROWTH (GNSS Reflectometry for Ocean Waves, Tides, and Height). It is aiming to evaluate the capabilities of GNSS-R observations for oceanographic phenomena with different time scales, such as ocean waves (1/10 to tens of seconds), tides (one or half days), and sea surface dynamic height (a few days to years). In situ observations of ocean wave spectrum, wind speed vertical profile, and sea surface height will be quantitatively compared with equivalent estimates from simultaneous GNSS-R measurements. The GROWTH project will utilize different types of observation platforms; marine observation towers (about 20 m height), multi-copters (about 100 to 150 m height), and much higher-altitude CYGNSS data. Cross-platform data, together with in situ oceanographic observations, will be compared after adequate temporal averaging that accounts differences of the footprint sizes and temporal and spatial scales of oceanographic phenomena. This paper will provide overview of the GROWTH project, preliminary test results, obtained by the multi-sensor platform at observation towers, suggest actual footprint sizes and identification of swell. Preparation status of a ground station which will be supplied to receive CYGNSS data

  15. Public health genomics and personalized prevention: lessons from the COGS project.

    Science.gov (United States)

    Pashayan, N; Hall, A; Chowdhury, S; Dent, T; Pharoah, P D P; Burton, H

    2013-11-01

    Using the principles of public health genomics, we examined the opportunities and challenges of implementing personalized prevention programmes for cancer at the population level. Our model-based estimates indicate that polygenic risk stratification can potentially improve the effectiveness and cost-effectiveness of screening programmes. However, compared with 'one-size-fits-all' screening programmes, personalized screening adds further layers of complexity to the organization of screening services and raises ethical, legal and social challenges. Before polygenic inheritance is translated into population screening strategy, evidence from empirical research and engagement with and education of the public and the health professionals are needed. © 2013 The Association for the Publication of the Journal of Internal Medicine.

  16. Integrating Public Health and Deliberative Public Bioethics: Lessons from the Human Genome Project Ethical, Legal, and Social Implications Program.

    Science.gov (United States)

    Meagher, Karen M; Lee, Lisa M

    2016-01-01

    Public health policy works best when grounded in firm public health standards of evidence and widely shared social values. In this article, we argue for incorporating a specific method of ethical deliberation--deliberative public bioethics--into public health. We describe how deliberative public bioethics is a method of engagement that can be helpful in public health. Although medical, research, and public health ethics can be considered some of what bioethics addresses, deliberative public bioethics offers both a how and where. Using the Human Genome Project Ethical, Legal, and Social Implications program as an example of effective incorporation of deliberative processes to integrate ethics into public health policy, we examine how deliberative public bioethics can integrate both public health and bioethics perspectives into three areas of public health practice: research, education, and health policy. We then offer recommendations for future collaborations that integrate deliberative methods into public health policy and practice.

  17. Biological Parameters and Molecular Markers of Clone CL Brener - The Reference Organism of the Trypanosoma cruzi Genome Project

    Directory of Open Access Journals (Sweden)

    Bianca Zingales

    1997-11-01

    Full Text Available Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. Some biological parameters of CL Brener were determined: (a the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT medium at 28oC is 58±13 hr; (b differentiation of epimastigotes to metacyclic trypomastigotes is obtained by incubation in LIT-20% Grace´s medium; (c trypomastigotes infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37oC; (d blood forms are highly infective to mice; (e blood forms are susceptible to nifurtimox and benznidazole. The molecular typing of CL Brener has been determined: (a isoenzymatic profiles are characteristic of zymodeme ZB; (b PCR amplification of a 24Sa ribosomal RNA sequence indicates it belongs to T. cruzi lineage 1; (c schizodeme, randomly amplified polymorphic DNA (RAPD and DNA fingerprinting analyses were performed

  18. Chromosome region-specific libraries for human genome analysis. Final progress report, 1 March 1991--28 February 1994

    Energy Technology Data Exchange (ETDEWEB)

    Kao, F.T.

    1994-04-01

    The objectives of this grant proposal include (1) development of a chromosome microdissection and PCR-mediated microcloning technology, (2) application of this microtechnology to the construction of region-specific libraries for human genome analysis. During this grant period, the authors have successfully developed this microtechnology and have applied it to the construction of microdissection libraries for the following chromosome regions: a whole chromosome 21 (21E), 2 region-specific libraries for the long arm of chromosome 2, 2q35-q37 (2Q1) and 2q33-q35 (2Q2), and 4 region-specific libraries for the entire short arm of chromosome 2, 2p23-p25 (2P1), 2p21-p23 (2P2), 2p14-p16 (wP3) and 2p11-p13 (2P4). In addition, 20--40 unique sequence microclones have been isolated and characterized for genomic studies. These region-specific libraries and the single-copy microclones from the library have been used as valuable resources for (1) isolating microsatellite probes in linkage analysis to further refine the disease locus; (2) isolating corresponding clones with large inserts, e.g. YAC, BAC, P1, cosmid and phage, to facilitate construction of contigs for high resolution physical mapping; and (3) isolating region-specific cDNA clones for use as candidate genes. These libraries are being deposited in the American Type Culture Collection (ATCC) for general distribution.

  19. Umatilla River Fish Passage Operations Project : Annual Progress Report October 2007 - September 2008.

    Energy Technology Data Exchange (ETDEWEB)

    Bronson, James P.; Loffink, Ken; Duke, Bill

    2008-12-31

    adult Pacific lamprey was trapped and released above the Westland ladder this year. The Threemile Dam west bank juvenile bypass was opened on March 11, 2008 in conjunction with water deliveries and continued through the summer. West Extension Irrigation District (WEID) discontinued diverting live flow on June 24, 2008 but the bypass remained open throughout the project year. The juvenile trap was not operated this project year.

  20. A high HIV-1 strain variability in London, UK, revealed by full-genome analysis: Results from the ICONIC project

    Science.gov (United States)

    Frampton, Dan; Gallo Cassarino, Tiziano; Raffle, Jade; Hubb, Jonathan; Ferns, R. Bridget; Waters, Laura; Tong, C. Y. William; Kozlakidis, Zisis; Hayward, Andrew; Kellam, Paul; Pillay, Deenan; Clark, Duncan; Nastouli, Eleni; Leigh Brown, Andrew J.

    2018-01-01

    Background & methods The ICONIC project has developed an automated high-throughput pipeline to generate HIV nearly full-length genomes (NFLG, i.e. from gag to nef) from next-generation sequencing (NGS) data. The pipeline was applied to 420 HIV samples collected at University College London Hospitals NHS Trust and Barts Health NHS Trust (London) and sequenced using an Illumina MiSeq at the Wellcome Trust Sanger Institute (Cambridge). Consensus genomes were generated and subtyped using COMET, and unique recombinants were studied with jpHMM and SimPlot. Maximum-likelihood phylogenetic trees were constructed using RAxML to identify transmission networks using the Cluster Picker. Results The pipeline generated sequences of at least 1Kb of length (median = 7.46Kb, IQR = 4.01Kb) for 375 out of the 420 samples (89%), with 174 (46.4%) being NFLG. A total of 365 sequences (169 of them NFLG) corresponded to unique subjects and were included in the down-stream analyses. The most frequent HIV subtypes were B (n = 149, 40.8%) and C (n = 77, 21.1%) and the circulating recombinant form CRF02_AG (n = 32, 8.8%). We found 14 different CRFs (n = 66, 18.1%) and multiple URFs (n = 32, 8.8%) that involved recombination between 12 different subtypes/CRFs. The most frequent URFs were B/CRF01_AE (4 cases) and A1/D, B/C, and B/CRF02_AG (3 cases each). Most URFs (19/26, 73%) lacked breakpoints in the PR+RT pol region, rendering them undetectable if only that was sequenced. Twelve (37.5%) of the URFs could have emerged within the UK, whereas the rest were probably imported from sub-Saharan Africa, South East Asia and South America. For 2 URFs we found highly similar pol sequences circulating in the UK. We detected 31 phylogenetic clusters using the full dataset: 25 pairs (mostly subtypes B and C), 4 triplets and 2 quadruplets. Some of these were not consistent across different genes due to inter- and intra-subtype recombination. Clusters involved 70 sequences, 19.2% of the dataset. Conclusions

  1. Chernobyl Studies Project: Working group 7.0, Environmental transport and health effects. Progress report, March--September 1994

    Energy Technology Data Exchange (ETDEWEB)

    Anspaugh, L.R.; Hendrickson, S.M. [eds.

    1994-12-01

    In April 1988, the US and the former-USSR signed a Memorandum of Cooperation (MOC) for Civilian Nuclear Reactor Safety; this MOC was a direct result of the accident at the Chernobyl Nuclear Power Plant Unit 4 and the following efforts by the two countries to implement a joint program to improve the safety of nuclear power plants and to understand the implications of environmental releases. A Joint Coordinating Committee for Civilian Nuclear Reactor Safety (JCCCNRS) was formed to implement the MOC. The JCCCNRS established many working groups; most of these were the responsibility of the Nuclear Regulatory Commission, as far as the US participation was concerned. The lone exception was Working Group 7 on Environmental Transport and Health Effects, for which the US participation was the responsibility of the US Department of Energy (DOE). The purpose of Working Group 7 was succintly stated to be, ``To develop jointly methods to project rapidly the health effects of any future nuclear reactor accident.`` To implement the work DOE then formed two subworking groups: 7.1 to address Environmental Transport and 7.2 to address Health Effects. Thus, the DOE-funded Chernobyl Studies Project began. The majority of the initial tasks for this project are completed or near completion. The focus is now turned to the issue of health effects from the Chernobyl accident. Currently, we are involved in and making progress on the case-control and co-hort studies of thyroid diseases among Belarussian children. Dosimetric aspects are a fundamental part of these studies. We are currently working to implement similar studies in Ukraine. A major part of the effort of these projects is supporting these studies, both by providing methods and applications of dose reconstruction and by providing support and equipment for the medical teams.

  2. REBL: design progress toward 16 nm half-pitch maskless projection electron beam lithography

    Science.gov (United States)

    McCord, Mark A.; Petric, Paul; Ummethala, Upendra; Carroll, Allen; Kojima, Shinichi; Grella, Luca; Shriyan, Sameet; Rettner, Charles T.; Bevis, Chris F.

    2012-03-01

    REBL (Reflective Electron Beam Lithography) is a novel concept for high speed maskless projection electron beam lithography. Originally targeting 45 nm HP (half pitch) under a DARPA funded contract, we are now working on optimizing the optics and architecture for the commercial silicon integrated circuit fabrication market at the equivalent of 16 nm HP. The shift to smaller features requires innovation in most major subsystems of the tool, including optics, stage, and metrology. We also require better simulation and understanding of the exposure process. In order to meet blur requirements for 16 nm lithography, we are both shrinking the pixel size and reducing the beam current. Throughput will be maintained by increasing the number of columns as well as other design optimizations. In consequence, the maximum stage speed required to meet wafer throughput targets at 16 nm will be much less than originally planned for at 45 nm. As a result, we are changing the stage architecture from a rotary design to a linear design that can still meet the throughput requirements but with more conventional technology that entails less technical risk. The linear concept also allows for simplifications in the datapath, primarily from being able to reuse pattern data across dies and columns. Finally, we are now able to demonstrate working dynamic pattern generator (DPG) chips, CMOS chips with microfabricated lenslets on top to prevent crosstalk between pixels.

  3. Hydrologic Resources Management Program and Underground Test Area Project FY2005 Progress Report

    International Nuclear Information System (INIS)

    Eaton, G F; Genetti, V; Hu, Q; Hudson, G B; Kersting, A B; Lindvall, R E; Moran, J E; Nimz, G J; Ramon, E C; Rose, T P; Shuller, L; Williams, R W; Zavarin, M; Zhao, P

    2007-01-01

    This report describes FY 2005 technical studies conducted by the Chemical Biology and Nuclear Science Division (CBND) at Lawrence Livermore National Laboratory (LLNL) in support of the Hydrologic Resources Management Program (HRMP) and the Underground Test Area Project (UGTA). These programs are administered by the U.S. Department of Energy, National Nuclear Security Administration, Nevada Site Office (NNSA/NSO) through the Defense Programs and Environmental Restoration Divisions, respectively. HRMP-sponsored work is directed toward the responsible management of the natural resources at the Nevada Test Site (NTS), enabling its continued use as a staging area for strategic operations in support of national security. UGTA-funded work emphasizes the development of an integrated set of groundwater flow and contaminant transport models to predict the extent of radionuclide migration from underground nuclear testing areas at the NTS. The report is organized on a topical basis and contains five chapters that highlight technical work products produced by CBND. However, it is important to recognize that most of this work involves collaborative partnerships with the other HRMP and UGTA contract organizations. These groups include the Energy and Environment Directorate at LLNL (LLNL-E and E), Los Alamos National Laboratory (LANL), the Desert Research Institute (DRI), the U.S. Geological Survey (USGS), Stoller-Navarro Joint Venture (SNJV), and Bechtel Nevada (BN)

  4. MHD Integrated Topping Cycle Project. Thirteenth quarterly technical progress report, August 1, 1990--October 31, 1990

    Energy Technology Data Exchange (ETDEWEB)

    1992-01-01

    The overall objective of the project is to design and construct prototypical hardware for an integrated MHD topping cycle, and conduct long duration proof-of-concept tests of integrated system at the US DOE Component Development and Integration Facility in Butte, Montana. The results of the long duration tests will augment the existing engineering design data base on MHD power train reliability, availability, maintainability, and performance, and will serve as a basis for scaling up the topping cycle design to the next level of development, an early commercial scale power plant retrofit. The components of the MHD power train to be designed, fabricated, and tested include: A slagging coal combustor with a rated capacity of 50 MW thermal input, capable of operation with an Eastern (Illinois {number_sign}6) or Western (Montana Rosebud) coal, a segmented supersonic nozzle, a supersonic MHD channel capable of generating at least 1.5 MW of electrical power, a segmented supersonic diffuser section to interface the channel with existing facility quench and exhaust systems, a complete set of current control circuits for local diagonal current control along the channel, and a set of current consolidation circuits to interface the channel with the existing facility inverter.

  5. Synchrotron topographic project. Progress report, February 20, 1981-January 20, 1982

    International Nuclear Information System (INIS)

    Bilello, J.C.

    1982-01-01

    The Synchrotron Topography Project (STP) has under design and construction various phases of a dedicated beam line for x-ray diffraction topography users in conjunction with the National Synchrotron Light Source at Brookhaven National Laboratory. During the past year final design and procurement phase has been completed for the following: (1) Experimental Hutch, (2) White Beam Camera, (3) Detector Arm for White Beam Camera, (4) Film Cassette System, (5) Medium Resolution Real-time TV System, (6) Lift Table Assembly, (7) Asymmetric Camera Base Mount, (8) Motor Control System, and (9) Computer system. Experimental work has been initiated on using reflection topography to study fracture surfaces. Preliminary results, both with Berg-Barrett, as well as with Synchrotron Topography done in collaboration with the Daresbury, U.K. facility show that defects generated in the near surface layers can be detected. Research work on the effects of stress concentration and geometric effects due to grain boundaries on the fracture of tungsten has been completed

  6. Greenhouse gas emission trends and projections in Europe 2012. Tracking progress towards Kyoto and 2020 targets

    Energy Technology Data Exchange (ETDEWEB)

    Gores, S.; Scheffler, M.; Graichen, V. [Oeko-Institut (Oeko), Freiburg (Germany)] [and others

    2012-10-15

    At the end of 2011, almost all European countries were on track towards their Kyoto targets for 2008-2012. The EU-15 also remained on track to achieve its Kyoto target. Italy, however, was not on track. Spain plans to acquire a large quantity of Kyoto units through the KP's flexible mechanisms to achieve its target. With emission caps already set for the economic sectors under the EU Emissions Trading Scheme (EU ETS), emissions reductions during 2012 in the sectors outside the EU ETS together with reductions by carbon sinks will set the frame for how many Kyoto units Member States need to acquire to reach their individual targets. Hence, both the development and delivery of adequate plans to acquire enough Kyoto credits is becoming increasingly important. ETS emissions from 2008 to 2011 were on average 5 % below these caps, which results in an oversupply of allowances. The EU ETS is undergoing important changes in view of the third trading phase from 2013 to 2020. Most EU Member States project that in 2020, their emissions outside the EU ETS will be lower than their national targets set under the Climate and Energy Package. However, further efforts will be necessary to achieve longer term reductions. (Author)

  7. Ceramic technology for advanced heat engines project: Semiannual progress report, October 1986-March 1987

    Energy Technology Data Exchange (ETDEWEB)

    1987-08-01

    This report contains four subelements: (1) Monolithics, (2) Ceramic Composites, (3) Thermal and Wear Coatings, and (4) Joining. Ceramic research conducted within the Monolithics subelement currently includes work activities on green state ceramic fabrication, characterization, and densification and on structural, mechanical, and physical properties of these ceramics. Research conducted within the Ceramic Composites subelement currently includes silicon carbide and oxide-based composites, which, in addition to the work activities cited for Monolithics, include fiber synthesis and characterization. Research conducted in the Thermal and Wear Coatings subelement is currently limited to oxide-base coatings and involves coating synthesis, characterization, and determination of the mechanical and physical properties of the coatings. Research conducted in the Joining subelement currently includes studies of processes to produce strong stable joints between zirconia ceramics and iron-base alloys. A major objective of the research in the Materials and Processing project element is to systematically advance the understanding of the relationships between ceramic raw materials such as powders and reactant gases, the processing variables involved in producing the ceramic materials, and the resultant microstructures and physical and mechanical properties of the ceramic materials. Success in meeting this objective will provide US companies with new or improved ways for producing economical highly reliable ceramic components for advanced heat engines.

  8. 1986 progress report on R and D work of the Nuclear Fusion Project (PKF)

    International Nuclear Information System (INIS)

    Kast, G.

    1987-03-01

    The Kernforschungszentrum Karlsruhe (KfK) and the Max-Planck-Institute for Plasma Physics, Garching, have joined to form the Entwicklungsgemeinschaft Kernfusion, and under this roof perform research work on the physical and technical conditions and requirements of using the energy from nuclear fusion, and to plan the design and the construction of the necessary equipment for future large-scale experiments. The activities of the KfK for the development of fusion devices with magnetic confinement have been concentrated in the Nuclear Fusion Project, which cooperates in the European Fusion Technology Programme, on the basis of an association agreement between KfK and Euratom, supported by the European Commission. Supplementary KfK contributions, studies in most cases, create a connection between various fields of work, and prepare the road for new tasks, which in the years to come will be found in the extension of the Fusion Technology Programme and the planning work for NET. The reports collected in this annual survey are contributions from KfK Institutes of the year 1986. An annex presents the Easks taken over by KfK within the Fusion Technology Programme of the EC, and notes on the KfK Institutes taking part in the particular activities facilitate reference to the individual, full-text reports. (orig.) [de

  9. Opportunities and challenges for the integration of massively parallel genomic sequencing into clinical practice: lessons from the ClinSeq project.

    Science.gov (United States)

    Biesecker, Leslie G

    2012-04-01

    The debate surrounding the return of results from high-throughput genomic interrogation encompasses many important issues including ethics, law, economics, and social policy. As well, the debate is also informed by the molecular, genetic, and clinical foundations of the emerging field of clinical genomics, which is based on this new technology. This article outlines the main biomedical considerations of sequencing technologies and demonstrates some of the early clinical experiences with the technology to enable the debate to stay focused on real-world practicalities. These experiences are based on early data from the ClinSeq project, which is a project to pilot the use of massively parallel sequencing in a clinical research context with a major aim to develop modes of returning results to individual subjects. The study has enrolled >900 subjects and generated exome sequence data on 572 subjects. These data are beginning to be interpreted and returned to the subjects, which provides examples of the potential usefulness and pitfalls of clinical genomics. There are numerous genetic results that can be readily derived from a genome including rare, high-penetrance traits, and carrier states. However, much work needs to be done to develop the tools and resources for genomic interpretation. The main lesson learned is that a genome sequence may be better considered as a health-care resource, rather than a test, one that can be interpreted and used over the lifetime of the patient.

  10. Snake River Sockeye Salmon Captive Broodstock Program Hatchery Element : Project Progress Report 2007 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Baker, Dan J.; Heindel, Jeff A.; Green, Daniel G.; Kline, Paul A.

    2008-12-17

    Numbers of Snake River sockeye salmon Oncorhynchus nerka have declined dramatically in recent years. In Idaho, only the lakes of the upper Salmon River (Sawtooth Valley) remain as potential sources of production (Figure 1). Historically, five Sawtooth Valley lakes (Redfish, Alturas, Pettit, Stanley, and Yellowbelly) supported sockeye salmon (Bjornn et al. 1968; Chapman et al. 1990). Currently, only Redfish Lake receives a remnant anadromous run. On April 2, 1990, the National Oceanic and Atmospheric Administration Fisheries Service (NOAA - formerly National Marine Fisheries Service) received a petition from the Shoshone-Bannock Tribes (SBT) to list Snake River sockeye salmon as endangered under the United States Endangered Species Act (ESA) of 1973. On November 20, 1991, NOAA declared Snake River sockeye salmon endangered. In 1991, the SBT, along with the Idaho Department of Fish & Game (IDFG), initiated the Snake River Sockeye Salmon Sawtooth Valley Project (Sawtooth Valley Project) with funding from the Bonneville Power Administration (BPA). The goal of this program is to conserve genetic resources and to rebuild Snake River sockeye salmon populations in Idaho. Coordination of this effort is carried out under the guidance of the Stanley Basin Sockeye Technical Oversight Committee (SBSTOC), a team of biologists representing the agencies involved in the recovery and management of Snake River sockeye salmon. National Oceanic and Atmospheric Administration Fisheries Service ESA Permit Nos. 1120, 1124, and 1481 authorize IDFG to conduct scientific research on listed Snake River sockeye salmon. Initial steps to recover the species involved the establishment of captive broodstocks at the Eagle Fish Hatchery in Idaho and at NOAA facilities in Washington State (for a review, see Flagg 1993; Johnson 1993; Flagg and McAuley 1994; Kline 1994; Johnson and Pravecek 1995; Kline and Younk 1995; Flagg et al. 1996; Johnson and Pravecek 1996; Kline and Lamansky 1997; Pravecek and

  11. SCREENING LOW FREQUENCY SNPS FROM GENOME WIDE ASSOCIATION STUDY REVEALS A NEW RISK ALLELE FOR PROGRESSION TO AIDS

    Science.gov (United States)

    Le Clerc, Sigrid; Coulonges, Cédric; Delaneau, Olivier; Van Manen, Danielle; Herbeck, Joshua T.; Limou, Sophie; An, Ping; Martinson, Jeremy J.; Spadoni, Jean-Louis; Therwath, Amu; Veldink, Jan H.; van den Berg, Leonard H.; Taing, Lieng; Labib, Taoufik; Mellak, Safa; Montes, Matthieu; Delfraissy, Jean-François; Schächter, François; Winkler, Cheryl; Froguel, Philippe; Mullins, James I.; Schuitemaker, Hanneke; Zagury, Jean-François

    2011-01-01

    Background Seven genome-wide association studies (GWAS) have been published in AIDS and only associations in the HLA region on chromosome 6 and CXCR6 have passed genome-wide significance. Methods We reanalyzed the data from three previously published GWAS, targeting specifically low frequency SNPs (minor allele frequency (MAF)<5%). Two groups composed of 365 slow progressors (SP) and 147 rapid progressors (RP) from Europe and the US were compared with a control group of 1394 seronegative individuals using Eigenstrat corrections. Results Of the 8584 SNPs with MAF<5% in cases and controls (Bonferroni threshold=5.8×10−6), four SNPs showed statistical evidence of association with the SP phenotype. The best result was for HCP5 rs2395029 (p=8.54×10−15, OR=3.41) in the HLA locus, in partial linkage disequilibrium with two additional chromosome 6 associations in C6orf48 (p=3.03×10−10, OR=2.9) and NOTCH4 (9.08×10−07, OR=2.32). The fourth association corresponded to rs2072255 located in RICH2 (p=3.30×10−06, OR=0.43) in chromosome 17. Using HCP5 rs2395029 as a covariate, the C6orf48 and NOTCH4 signals disappeared, but the RICH2 signal still remained significant. Conclusion Besides the already known chromosome 6 associations, the analysis of low frequency SNPs brought up a new association in the RICH2 gene. Interestingly, RICH2 interacts with BST-2 known to be a major restriction factor for HIV-1 infection. Our study has thus identified a new candidate gene for AIDS molecular etiology and confirms the interest of singling out low frequency SNPs in order to exploit GWAS data. PMID:21107268

  12. Geothermal direct-heat utilization assistance. Federal Assistance Program quarterly project progress report, April 1--June 30, 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-07-01

    This report summarizes geothermal technical assistance, R and D and technology transfer activities of the Geo-Heat Center at Oregon Institute of Technology for the third quarter of FY98 (April--June, 1998). It describes 231 contacts with parties during this period related to technical assistance with geothermal direct heat projects. Areas dealt with included requests for general information including material for high school and university students, and material on geothermal heat pumps, resource and well data, spacing heating and cooling, greenhouses, aquaculture, equipment, district heating, resorts and spas, industrial applications, snow melting and electric power. Research activities include work on model construction specifications for line shaft submersible pumps and plate heat exchangers, and a comprehensive aquaculture developers package. A brochure on Geothermal Energy in Klamath County was developed for state and local tourism use. Outreach activities include the publication of the Quarterly Bulletin (Vol. 19, No. 2) with articles on research at the Geo-Heat Center, sustainability of geothermal resources, injection well drilling in Boise, ID and a greenhouse project in the Azores. Other outreach activities include dissemination of information mainly through mailings of publications, tours of local geothermal uses, geothermal library acquisitions and use, participation in workshops, short courses and technical meetings by the staff, and progress monitor reports on geothermal activities.

  13. Development of a Methodology for Hydrogeological Characterization of Faults: Progress of the Project in Berkeley, California

    Science.gov (United States)

    Goto, J.; Moriya, T.; Yoshimura, K.; Tsuchi, H.; Karasaki, K.; Onishi, T.; Ueta, K.; Tanaka, S.; Kiho, K.

    2010-12-01

    The Nuclear Waste Management Organization of Japan (NUMO), in collaboration with Lawrence Berkeley National Laboratory (LBNL), has carried out a project to develop an efficient and practical methodology to characterize hydrologic property of faults since 2007, exclusively for the early stage of siting a deep underground repository. A preliminary flowchart of the characterization program and a classification scheme of fault hydrology based on the geological feature have been proposed. These have been tested through the field characterization program on the Wildcat Fault in Berkeley, California. The Wildcat Fault is a relatively large non-active strike-slip fault which is believed to be a subsidiary of the active Hayward Fault. Our classification scheme assumes the contrasting hydrologic features between the linear northern part and the split/spread southern part of the Wildcat Fault. The field characterization program to date has been concentrated in and around the LBNL site on the southern part of the fault. Several lines of electrical and reflection seismic surveys, and subsequent trench investigations, have revealed the approximate distribution and near-surface features of the Wildcat Fault (see also Onishi, et al. and Ueta, et al.). Three 150m deep boreholes, WF-1 to WF-3, have been drilled on a line normal to the trace of the fault in the LBNL site. Two vertical holes were placed to characterize the undisturbed Miocene sedimentary formations at the eastern and western sides of the fault (WF-1 and WF-2 respectively). WF-2 on the western side intersected the rock formation, which was expected only in WF-1, and several of various intensities. Therefore, WF-3, originally planned as inclined to penetrate the fault, was replaced by the vertical hole further to the west. It again encountered unexpected rocks and faults. Preliminary results of in-situ hydraulic tests suggested that the transmissivity of WF-1 is ten to one hundred times higher than WF-2. The monitoring

  14. Planning the Human Variome Project : The Spain Report

    NARCIS (Netherlands)

    Kaput, Jim; Cotton, Richard G. H.; Hardman, Lauren; Watson, Michael; Al Aqeel, Aida I.; Al-Aama, Jumana Y.; Al-Mulla, Fahd; Alonso, Santos; Aretz, Stefan; Auerbach, Arleen D.; Bapat, Bharati; Bernstein, Inge T.; Bhak, Jong; Bleoo, Stacey L.; Bloecker, Helmut; Brenner, Steven E.; Burn, John; Bustamante, Mariona; Calone, Rita; Cambon-Thomsen, Anne; Cargill, Michele; Carrera, Paola; Cavedon, Lawrence; Cho, Yoon Shin; Chung, Yeun-Jun; Claustres, Mireille; Cutting, Garry; Dalgleish, Raymond; den Dunnen, Johan T.; Diaz, Carlos; Dobrowolski, Steven; dos Santos, M. Rosario N.; Ekong, Rosemary; Flanagan, Simon B.; Flicek, Paul; Furukawa, Yoichi; Genuardi, Maurizio; Ghang, Ho; Golubenko, Maria V.; Greenblatt, Marc S.; Hamosh, Ada; Hancock, John M.; Hardison, Ross; Harrison, Terence M.; Hoffmann, Robert; Horaitis, Rania; Howard, Heather J.; Barash, Carol Isaacson; Izagirre, Neskuts; Sijmons, Rolf H.

    The remarkable progress in characterizing the human genome sequence, exemplified by the Human Genome Project and the HapMap Consortium, has led to the perception that knowledge and the tools (e.g., microarrays) are sufficient for many if not most biomedical research efforts. A large amount of data

  15. Planning the human variome project: the Spain report

    DEFF Research Database (Denmark)

    Kaput, Jim; Cotton, Richard G H; Hardman, Lauren

    2009-01-01

    The remarkable progress in characterizing the human genome sequence, exemplified by the Human Genome Project and the HapMap Consortium, has led to the perception that knowledge and the tools (e.g., microarrays) are sufficient for many if not most biomedical research efforts. A large amount of dat...

  16. Planning the human variome project: the Spain report.

    NARCIS (Netherlands)

    Kaput, J.; Cotton, R.G.; Hardman, L.; Watson, M.; Aqeel, A.I. Al; Al-Aama, J.Y.; Al-Mulla, F.; Alonso, S.; Aretz, S.; Auerbach, A.D.; Bapat, B.; Bernstein, I.T.; Bhak, J.; Bleoo, S.L.; Blocker, H.; Brenner, S.E.; Burn, J.; Bustamante, M.; Calzone, R.; Cambon-Thomsen, A.; Cargill, M.; Carrera, P.; Cavedon, L.; Cho, Y.S.; Chung, Y.J.; Claustres, M.; Cutting, G.; Dalgleish, R.; Dunnen, J.T. den; Diaz, C.; Dobrowolski, S.; Santos, M.R. dos; Ekong, R.; Flanagan, S.B.; Flicek, P.; Furukawa, Y.; Genuardi, M.; Ghang, H.; Golubenko, M.V.; Greenblatt, M.S.; Hamosh, A.; Hancock, J.M.; Hardison, R.; Harrison, T.M.; Hoffmann, R.; Horaitis, R.; Howard, H.J.; Barash, C.I.; Izagirre, N.; Jung, J.; Kojima, T.; Laradi, S.; Lee, Y.S.; Lee, J.Y.; Gil-da-Silva-Lopes, V.L.; Macrae, F.A.; Maglott, D.; Marafie, M.J.; Marsh, S.G.; Matsubara, Y.; Messiaen, L.M.; Moslein, G.; Netea, M.G.; Norton, M.L.; Oefner, P.J.; Oetting, W.S.; O'Leary, J.C.; Ramirez, A.M. de; Paalman, M.H.; Parboosingh, J.; Patrinos, G.P.; Perozzi, G.; Phillips, I.R.; Povey, S.; Prasad, S.; Qi, M.; Quin, D.J.; Ramesar, R.S.; Richards, C.S.; Savige, J.; Scheible, D.G.; Scott, R.J.; Seminara, D.; Shephard, E.A.; Sijmons, R.H.; Smith, T.D.; Sobrido, M.J.; Tanaka, T.; Tavtigian, S.V.; Taylor, G.R.; Teague, J.; Topel, T.; Ullman-Cullere, M.; Utsunomiya, J.; Kranen, H.J. van; Vihinen, M.; Webb, E.; Weber, T.K.; Yeager, M.

    2009-01-01

    The remarkable progress in characterizing the human genome sequence, exemplified by the Human Genome Project and the HapMap Consortium, has led to the perception that knowledge and the tools (e.g., microarrays) are sufficient for many if not most biomedical research efforts. A large amount of data

  17. REGIA, An EU Project on Functional Genomics of Transcription Factors from Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Javier Paz-Ares

    2002-01-01

    and metabolic profiling; 5. the systematic analysis of interactions between TFs; and 6. the generation of a bioinformatics infrastructure to access and integrate all this information. We expect that this programme will establish the full biotechnological potential of plant TFs, and provide insights into hierarchies, redundancies, and interdependencies, and their evolution. The project involves the preparation of both a TF gene array for expression analysis and a normalised full length open reading frame (ORF library of TFs in a yeast two hybrid vector; the applications of these resources should extend beyond the scope of this programme.

  18. Hydrologic Resources Management Program and Underground Test Area Project FY 2001-2002 Progress Report

    International Nuclear Information System (INIS)

    Rose, T.P.; Kersting, A.B.; Harris, L.J.; Hudson, G.B.; Smith, D.K.; Williams, R.W.; Loewen, D.R.; Nelson, E.J.; Allen, P.G.; Ryerson, F.J.; Pawloski, G.A.; Laue, C.A.; Moran, J.E.

    2003-01-01

    This report contains highlights of FY 2001 and 2002 technical studies conducted by the Analytical and Nuclear Chemistry Division (ANCD) at Lawrence Livermore National Laboratory (LLNL) in support of the Hydrologic Resources Management Program (HRMP) and the Underground Test Area (UGTA) Project. These programs are administered by the U.S. Department of Energy, National Nuclear Security Administration, Nevada Site Office (NNSA/NSO) through the Defense Programs and Environmental Restoration Divisions, respectively. HRMP-sponsored work emphasizes the Defense Programs goal of responsible management of natural resources at the NTS, while UGTA-funded work focuses on defining the extent of radionuclide contamination in NTS groundwater resulting from underground nuclear testing. The report is organized on a topical basis, and contains eight chapters that reflect the range of technical work performed by LLNL-ANCD in support of HRMP and UGTA. Chapter 1 describes recent hot well sampling efforts at the NTS, and presents the results of chemical and isotopic analyses of groundwater samples from six near-field wells. These include the Cambric (UE-5n), Bilby (U-3cn PS No.2), Bourbon (UE-7nS), Nash (UE-2ce), Tybo/Benham (ER-20-5 No.3), and Almendro (U-19v PS No.1ds) sites. The data generated by the hot well program is vital to the development and validation of contaminant transport models at the NTS. Chapter 2 discusses the results of xenon isotope measurements of groundwater samples from the six near-field wells described in Chapter 1. This work demonstrates that fission xenon is present in the water at levels that are readily measurable and highlights the significant differences in xenon concentrations and isotopic abundances at different sites. These differences provide insight into the early cooling history of nuclear test cavities, and may assist in predicting the distribution of the source term in the near-field environment. Chapter 3 is an investigation of the distribution

  19. Balancing Benefits and Risks of Immortal Data: Participants' Views of Open Consent in the Personal Genome Project.

    Science.gov (United States)

    Zarate, Oscar A; Brody, Julia Green; Brown, Phil; Ramirez-Andreotta, Mónica D; Perovich, Laura; Matz, Jacob

    2016-01-01

    An individual's health, genetic, or environmental-exposure data, placed in an online repository, creates a valuable shared resource that can accelerate biomedical research and even open opportunities for crowd-sourcing discoveries by members of the public. But these data become "immortalized" in ways that may create lasting risk as well as benefit. Once shared on the Internet, the data are difficult or impossible to redact, and identities may be revealed by a process called data linkage, in which online data sets are matched to each other. Reidentification (re-ID), the process of associating an individual's name with data that were considered deidentified, poses risks such as insurance or employment discrimination, social stigma, and breach of the promises often made in informed-consent documents. At the same time, re-ID poses risks to researchers and indeed to the future of science, should re-ID end up undermining the trust and participation of potential research participants. The ethical challenges of online data sharing are heightened as so-called big data becomes an increasingly important research tool and driver of new research structures. Big data is shifting research to include large numbers of researchers and institutions as well as large numbers of participants providing diverse types of data, so the participants' consent relationship is no longer with a person or even a research institution. In addition, consent is further transformed because big data analysis often begins with descriptive inquiry and generation of a hypothesis, and the research questions cannot be clearly defined at the outset and may be unforeseeable over the long term. In this article, we consider how expanded data sharing poses new challenges, illustrated by genomics and the transition to new models of consent. We draw on the experiences of participants in an open data platform-the Personal Genome Project-to allow study participants to contribute their voices to inform ethical consent

  20. New genomic structure for prostate cancer specific gene PCA3 within BMCC1: implications for prostate cancer detection and progression.

    Directory of Open Access Journals (Sweden)

    Raymond A Clarke

    Full Text Available The prostate cancer antigen 3 (PCA3/DD3 gene is a highly specific biomarker upregulated in prostate cancer (PCa. In order to understand the importance of PCA3 in PCa we investigated the organization and evolution of the PCA3 gene locus.We have employed cDNA synthesis, RTPCR and DNA sequencing to identify 4 new transcription start sites, 4 polyadenylation sites and 2 new differentially spliced exons in an extended form of PCA3. Primers designed from these novel PCA3 exons greatly improve RT-PCR based discrimination between PCa, PCa metastases and BPH specimens. Comparative genomic analyses demonstrated that PCA3 has only recently evolved in an anti-sense orientation within a second gene, BMCC1/PRUNE2. BMCC1 has been shown previously to interact with RhoA and RhoC, determinants of cellular transformation and metastasis, respectively. Using RT-PCR we demonstrated that the longer BMCC1-1 isoform - like PCA3 - is upregulated in PCa tissues and metastases and in PCa cell lines. Furthermore PCA3 and BMCC1-1 levels are responsive to dihydrotestosterone treatment.Upregulation of two new PCA3 isoforms in PCa tissues improves discrimination between PCa and BPH. The functional relevance of this specificity is now of particular interest given PCA3's overlapping association with a second gene BMCC1, a regulator of Rho signalling. Upregulation of PCA3 and BMCC1 in PCa has potential for improved diagnosis.