WorldWideScience

Sample records for generation isol facilities

  1. An advanced ISOL facility based on ATLAS

    CERN Document Server

    Nolen, J A; Pardo, R C; Savard, G; Rehm, K E; Schiffer, J P; Henning, W F; Jiang, C L; Ahmad, L; Back, B B; Kaye, R A; Petra, M; Portillo, M; Greene, J; Clifft, B E; Specht, J R; Janssens, R V F; Siemssen, R H; Gómez, I; Reed, C B; Hassanein, A M

    1999-01-01

    The Argonne concept for an accelerator complex for efficiently producing high-quality radioactive beams from an ion source energy up to 6-15 MeV/u is described. The Isotope-Separator-On-Line (ISOL) method is used. A high-power $9 driver accelerator produces radionuclides in a target that is closely coupled to an ion source and mass separator. By using a driver accelerator which can deliver a variety of beams and energies the radionuclide production mechanisms $9 can be chosen to optimize yields for the species of interest. To effectively utilize the high beam power of the driver two-step target /ion source geometries are proposed: (1) Neutron production with intermediate energy deuterons on $9 a primary target to produce neutron- rich fission products in a secondary /sup 238/U target, and (2) Fragmentation of neutron-rich heavy ion beams such as /sup 18/O in a target/catcher geometry. Heavy ion beams with total energies in $9 the 1-10 GeV range are also available for radionuclide production via high-energy sp...

  2. A Vibration Isolation System for Use in a Large Thermal Vacuum Test Facility

    Science.gov (United States)

    Hershfeld, Donald; VanCampen, Julie

    2002-01-01

    A thermal vacuum payload platform that is isolated from background vibration is required to support the development of future instruments for Hubble Space Telescope (HST) and the Next Generation Space Telescope (NGST) at the Goddard Space Flight Center (GSFC). Because of the size and weight of the thermal/vacuum facility in which the instruments are tested, it is not practical to isolate the entire facility externally. Therefore, a vibration isolation system has been designed and fabricated to be installed inside the chamber. The isolation system provides a payload interface of 3.05 m (10 feet) in diameter and is capable of supporting a maximum payload weight of 4536 kg (10,000 Lbs). A counterweight system has been included to insure stability of payloads having high centers of gravity. The vibration isolation system poses a potential problem in that leakage into the chamber could compromise the ability to maintain vacuum. Strict specifications were imposed on the isolation system design to minimize leakage. Vibration measurements, obtained inside the chamber, prior to installing the vibration isolation system, indicated levels in all axes of approximately 1 milli-g at about 20 Hz. The vibration isolation system was designed to provide a minimum attenuation of 40 dB to these levels. This paper describes the design and testing of this unique vibration isolation system. Problems with leakage and corrective methods are presented. Isolation performance results are also presented.

  3. Upgrading hydro-electric generating facilities

    Energy Technology Data Exchange (ETDEWEB)

    Coulson, D.M.; Villalon, M.

    1987-11-01

    Approximately 1,720,000 kW of installed hydro-electric generating capacity is either owned directly by pulp and paper companies in Canada or is operated principally in their service. The 216 units involved were commissioned between 1902 and 1982. This paper suggests an approach to upgrading the generating units themselves as well as the related electrical and mechanical equipment and civil structures, giving approximate durations and costs. A complete system study is recommended. 2 refs., 2 figs., 3 tabs.

  4. 7 CFR 1726.125 - Generating plant facilities.

    Science.gov (United States)

    2010-01-01

    ..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Generation Facilities § 1726... design, RUS will, in consultation with the borrower and its consulting engineer, identify the specific... desulfurization system, particulate removal system, electric wiring and control systems, mechanical...

  5. Cold Vacuum Drying (CVD) Facility, Diesel Generator Fire Protection

    CERN Document Server

    Singh, G

    2000-01-01

    This Acceptance Test Procedure (ATP) has been prepared to demonstrate that the Fire Protection and Detection System installed by Project W-441 (Cold Vacuum Drying Facility and Diesel Generator Building) functions as required by project specifications.

  6. Development of a neutron generator facility at Simon Fraser University

    OpenAIRE

    2015-01-01

    A new neutron generator facility at Simon Fraser University (SFU) utilizes a commercial deuterium-tritium neutron generator (Thermo Scientific P 385) to produce 14.2 MeV neutrons at a nominal rate of 3E8 neutrons/s. The facility will be used to produce radioisotopes to support a research program including nuclear structure studies and neutron activation analysis. As a prerequisite for regular operation of the facility and as a personnel safety consideration, dose rate predictions for the fa...

  7. Study of parameters of a facility generating compressive plasma flows

    Science.gov (United States)

    Leyvi, A. Ya

    2017-05-01

    The prosperity of plasma technologies stimulates making of a facility generating compressive plasma flows at the South Ural State University. The facility is a compact-geometry magnetoplasma compressor with the following parameters: stored energy up to 15 kJ, voltage of a bank from 3 to 5 kV; nitrogen, air, and other gases can serve as its operating gas. The investigation of parameters of the facility showed the following parameters of compressive plasma flows: impulse duration of up to 120 μs, discharge current of 50-120 kA, speed of plasma flow of 15-30 km/s. By contrast to the available facilities, the parameters of the developed facility can be adjusted in a wide range of voltage from 2 kV to 10 kV, its design permits generating CPF in horizontal and vertical positions.

  8. Recent developments at the ISOL facility of GSI Darmstadt

    CERN Document Server

    Roeckl, E; Burkard, K; Döring, J; Grawe, H; Hüller, W; Kirchner, R; Mazzocchi, C; Mukha, I; Plettner, C

    2003-01-01

    The research programme pursued at the ISOL facility of GSI Darmstadt focuses on the study of decay properties of nuclei along the N congruent with Z line between the double shell closures at sup 5 sup 6 Ni and sup 1 sup 0 sup 0 Sn. In this report, the major scientific achievements obtained in the past two years are reviewed, with particular emphasis being put on the detection techniques.

  9. Test facility of thermal storage equipment for space power generation

    Science.gov (United States)

    Inoue, T.; Nakagawa, M.; Mochida, Y.; Ohtomo, F.; Shimizu, K.; Tanaka, K.; Abe, Y.; Nomura, O.; Kamimoto, M.

    A thermal storage equipment test facility has been built in connection with developing solar dynamic power systems (SDPSs). The test facility consists of a recuperative closed Brayton cycle system (CBC), with a mixture of helium and xenon with a molecular weight of 39.9 serving as the working fluid. CBC has been shown to be the most attractive power generation system among several types of SDPSs because of its ability to meet the required high power demand and its thermal efficiency, about 30 percent. The authors present a description of this test facility and give results of the preliminary test and the first-stage test with heat storage equipment.

  10. Transmission cost minimization strategies for wind-electric generating facilities

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez, R. [Northern States Power Company, Minneapolis, MN (United States)

    1997-12-31

    Integrating wind-electric generation facilities into existing power systems presents opportunities not encountered in conventional energy projects. Minimizing outlet cost requires probabilistic value-based analyses appropriately reflecting the wind facility`s operational characteristics. The wind resource`s intermittent nature permits relaxation of deterministic criteria addressing outlet configuration and capacity required relative to facility rating. Equivalent capacity ratings of wind generation facilities being a fraction of installed nameplate rating, outlet design studies contingency analyses can concentrate on this fractional value. Further, given its non-dispatchable, low capacity factor nature, a lower level of redundancy in outlet facilities is appropriate considering the trifling contribution to output unreliability. Further cost reduction opportunities arise from {open_quotes}wind speed/generator power output{close_quotes} and {open_quotes}wind speed/overhead conductor rating{close_quotes} functions` correlation. Proper analysis permits the correlation`s exploitation to safely increase line ratings. Lastly, poor correlation between output and utility load may permit use of smaller conductors, whose higher (mostly off-peak) losses are economically justifiable.

  11. Integrated biofuel facility, with carbon dioxide consumption and power generation

    Energy Technology Data Exchange (ETDEWEB)

    Powell, E.E.; Hill, G.A. [Saskatchewan Univ., Saskatoon, SK (Canada). Dept. of Chemical Engineering

    2009-07-01

    This presentation provided details of an economical design for a large-scale integrated biofuel facility for coupled production of bioethanol and biodiesel, with carbon dioxide capture and power generation. Several designs were suggested for both batch and continuous culture operations, taking into account all costs and revenues associated with the complete plant integration. The microalgae species Chlorella vulgaris was cultivated in a novel photobioreactor (PBR) in order to consume industrial carbon dioxide (CO{sub 2}). This photosynthetic culture can also act as a biocathode in a microbial fuel cell (MFC), which when coupled to a typical yeast anodic half cell, results in a complete biological MFC. The photosynthetic MFC produces electricity as well as valuable biomass and by-products. The use of this novel photosynthetic microalgae cathodic half cell in an integrated biofuel facility was discussed. A series of novel PBRs for continuous operation can be integrated into a large-scale bioethanol facility, where the PBRs serve as cathodic half cells and are coupled to the existing yeast fermentation tanks which act as anodic half cells. These coupled MFCs generate electricity for use within the biofuel facility. The microalgae growth provides oil for biodiesel production, in addition to the bioethanol from the yeast fermentation. The photosynthetic cultivation in the cathodic PBR also requires carbon dioxide, resulting in consumption of carbon dioxide from bioethanol production. The paper also discussed the effect of plant design on net present worth and internal rate of return. tabs., figs.

  12. A Second Generation Radioactive Nuclear Beam Facility at CERN

    CERN Document Server

    Äystö, J; Lindroos, M; Ravn, H L; Van Duppen, P

    2000-01-01

    The proposed Superconducting Proton Linac (SPL) at CERN would be an ideal driver for a proton-driven second-generation Radioactive Nuclear Beam facility. We propose to investigate the feasibility of constructing such a facility at CERN close to the present PS Booster ISOLDE facility. The existing ISOLDE facility would be fed with a 10 micro-amps proton beam from SPL, providing the physics community with a low-intensity experimental area. A second, new facility would be built with target stations deep underground, permitting proton beam intensities of more than 100 micro-amps. The secondary beams can be post-accelerated to 20-100 MeV/u and there will be a storage ring complex and large segmented detectors in the experimental area. Also, benefits from a muon-ion collider or from merging the ions and muons should be investigated. Since the antiproton decelerator would be nearby, the opportunities for antiprotonic radioactive atom studies should be pursued as well.

  13. Rainier Biogas Manure Management and Renewable Energy Generation Facility

    Energy Technology Data Exchange (ETDEWEB)

    Smyth, John [King County, WA (United States)

    2017-06-06

    The Rainier Biogas project is a community manure processing and renewable energy generation facility. Construction was completed and operation initiated in 2012. It is owned and operated by Rainier Biogas, LLC in collaboration with local dairy farmers, Washington State University, and the King County Department of Natural Resources and Parks. The project receives manure from three to four partner dairy farms mostly by underground pipe. The project is located at 43218 208th Ave SE; Enumclaw, WA 98022.

  14. Generation and release of radioactive gases in LLW disposal facilities

    Energy Technology Data Exchange (ETDEWEB)

    Yim, M.S. [Harvard School Public Health, Boston, MA (United States); Simonson, S.A. [Massachusetts Institute of Technology, Cambridge, MA (United States)

    1995-02-01

    The atmospheric release of radioactive gases from a generic engineered LLW disposal facility and its radiological impacts were examined. To quantify the generation of radioactive gases, detailed characterization of source inventory for carbon-14, tritium, iodine-129, krypton-85, and radon-222, was performed in terms of their activity concentrations; their distribution within different waste classes, waste forms and containers; and their subsequent availability for release in volatile or gaseous form. The generation of gases was investigated for the processes of microbial activity, radiolysis, and corrosion of waste containers and metallic components in wastes. The release of radionuclides within these gases to the atmosphere was analyzed under the influence of atmospheric pressure changes.

  15. Optical pulse generation system for the National Ignition Facility (NIF)

    Energy Technology Data Exchange (ETDEWEB)

    Penko, F; Braucht,; Browning, D; Crane, J K; Dane, B; Deadrick, F; Dreifuerst, G; Henesian, M; Jones, B A; Kot, L; Laumann, C; Martinez, M; Moran, B; Rothenberg, J E; Skulina, K; Wilcox, R B

    1998-06-18

    We describe the Optical Pulse Generation (OPG) system for the National Ignition Facility ( NIF ). The OPG system begins with the Master Oscillator Room ( MOR ) where the initial, seed pulse for the entire laser system is produced and properly formatted to enhance ignition in the target. The formatting consists of temporally shaping the pulse and adding additional bandwidth to increase the coupling of the laser generated x-rays to the high density target plasma. The pulse produced in the MOR fans out to 48 identical preamplifier modules where it is amplified by a factor of ten billion and spatially shaped for injection into the 192 main amplifier chai

  16. New facility in Hamilton to generate electricity from biosolids

    Energy Technology Data Exchange (ETDEWEB)

    Nolan, W. [Liberty Energy, Hamilton, ON (Canada); Thomson, M.; Ahluwalia, J. [Environ EC Canada, Mississauga, ON (Canada)

    2009-07-15

    Ontario's Green Energy Act was introduced in 2009 to facilitate progress toward greenhouse gas (GHG) reduction and to increase the amount of energy produced from renewable energy sources. This article described a technology that can be used to generate electricity from the biosolids that are generated at wastewater treatment plants and pulp and paper mills across Ontario. Liberty Energy Inc. is proposing to build a new renewable energy thermal electric power plant in Hamilton, Ontario. The facility will use waste biomass as fuel consisting of biosolids, or sewage sludge. These materials have traditionally been managed through land filling, land application, or incineration. The use of waste biomass for power generation will provide a long term, sustainable and environmentally friendly method to manage these waste materials. This article reviewed some of the thermal treatment technologies, including fixed hearth, multiple hearth, rotary kilns and fluidized bed reactors. The odour management plan for the facility includes sealed storage of biosolids and indoor receiving of both biosolids and biomass, with all venting to either the gasifier or a biofilter. The exhaust from the gasifier will be treated by selective catalytic and non-catalytic reduction technology, lime slurry wet scrubbers, fabric filters and powdered activated carbon scrubbers. 2 figs.

  17. Thermal hydraulic studies in steam generator test facility

    Energy Technology Data Exchange (ETDEWEB)

    Vinod, V.; Suresh Kumar, V.A.; Noushad, I.B.; Ellappan, T.R.; Rajan, K.K.; Rajan, M.; Vaidyanathan, G. [Engineering Development Group Indira Gandhi Centre for Atomic Research, Kalpakkam-603102 (India)

    2005-07-01

    Full text of publication follows: A 500 MWe fast breeder reactor is being constructed at Kalpakkam, India. This is a sodium cooled reactor with two primary and two secondary sodium loops with total 8 steam generators. The typical advantage of fast breeder plants is the high operating temperature of steam cycles and the high plant efficiency. To produce this high pressure and high temperature steam, once through straight tube vertical sodium heated steam generators are used. The steam is generated from the heat produced in the reactor core and being transported through primary and secondary sodium circuits. The steam generator is a 25 m high middle supported steam generator with expansion bend and 23 m heat transfer length. Steam Generator Test Facility (SGTF) constructed at Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam aims at performing various tests on a 5.5 MWt steam generator. This vertically simulated test article is similar in all respects to the proposed 157 MWt steam generator module for the Prototype Fast Breeder Reactor (PFBR), with reduced number of tubes. Heat transfer performance tests are done with this 19 tube steam generator at various load conditions. Sodium circuit for the SGTF is equipped with oil fired heater as heat source and centrifugal sodium pump, to pump sodium at 105 m{sup 3}/hr flow rate. Other typical components like sodium to air heat exchanger, sodium purification system and hydrogen leak detection system is also present in the sodium circuit. High pressure steam produced in the steam generator is dumped in a condenser and recycled. Important tests planned in SGTF are the heat transfer performance test, stability test, endurance test and performance test of steam generator under various transients. The controlled operation of steam generator will be studied with possible control schemes. A steady state simulation of the steam generator is done with a mathematical model. This paper gives the details of heat transfer

  18. Effect of liquid waste discharges from steam generating facilities

    Energy Technology Data Exchange (ETDEWEB)

    McGuire, H.E. Jr.

    1977-09-01

    This report contains a summary of the effects of liquid waste discharges from steam electric generating facilities on the environment. Also included is a simplified model for use in approximately determining the effects of these discharges. Four basic fuels are used in steam electric power plants: three fossil fuels--coal, natural gas, and oil; and uranium--presently the basic fuel of nuclear power. Coal and uranium are expected to be the major fuels in future years. The following power plant effluents are considered: heat, chlorine, copper, total dissolved solids, suspended solids, pH, oil and grease, iron, zinc, chrome, phosphorus, and trace radionuclides.

  19. Facility for generating crew waste water product for ECLSS testing

    Science.gov (United States)

    Buitekant, Alan; Roberts, Barry C.

    1990-01-01

    An End-use Equipment Facility (EEF) has been constructed which is used to simulate water interfaces between the Space Station Freedom Environmental Control and Life Support Systems (ECLSS) and man systems. The EEF is used to generate waste water to be treated by ECLSS water recovery systems. The EEF will also be used to close the water recovery loop by allowing test subjects to use recovered hygiene and potable water during several phases of testing. This paper describes the design and basic operation of the EEF.

  20. Facility for generating crew waste water product for ECLSS testing

    Science.gov (United States)

    Buitekant, Alan; Roberts, Barry C.

    1990-01-01

    An End-use Equipment Facility (EEF) has been constructed which is used to simulate water interfaces between the Space Station Freedom Environmental Control and Life Support Systems (ECLSS) and man systems. The EEF is used to generate waste water to be treated by ECLSS water recovery systems. The EEF will also be used to close the water recovery loop by allowing test subjects to use recovered hygiene and potable water during several phases of testing. This paper describes the design and basic operation of the EEF.

  1. Next Generation Gamma Ray Diagnostics for the National Ignition Facility

    Science.gov (United States)

    Herrmann, Hans; Kim, Y. H.; McEvoy, A. M.; Zylstra, A. B.; Young, C. S.; Lopez, F. E.; Griego, J. R.; Fatherley, V. E.; Oertel, J. A.; Jorgenson, H. J.; Barlow, D. B.; Stoeffl, W.; Church, J. A.; Hernandez, J. E.; Carpenter, A.; Rubery, M. S.; Horsfield, C. J.; Gales, S.; Leatherland, A.; Hilsabeck, T.; Kilkenny, J. D.; Malone, R. M.; Moy, K.; Hares, J. D.; Milnes, J.

    Fusion reaction history and ablator areal density measurements based on gamma ray detection are an essential part of Inertial Confinement Fusion (ICF) experiments on the National Ignition Facility (NIF). Capability improvements are being implemented in sensitivity, temporal and spectral response relative to the existing Gamma Reaction History diagnostic (GRH-6m). The ``Super'' Gas Cherenkov Detector (GCD) will provide 200x more sensitivity, reduce the effective temporal resolution from 100 to 10 ps, and lower the energy threshold from 2.9 to 1.8 MeV, relative to GRH-6m. The Gamma-to-Electron Magnetic Spectrometer (GEMS) - a Compton spectrometer intended to provide true gamma energy resolution (<=5%) for isolation of specific lines such as t(d, γ) , D(n, γ) , 12C(n,n' γ) and energetic charged particle nuclear reactions indicative of ablator/fuel mix

  2. Public radiation exposures from a CANDESAL Co-generation Facility

    Energy Technology Data Exchange (ETDEWEB)

    Khaloo, R. [Atomic Energy of Canada Limited, Mississauga, ON (Canada); Simanjuntak, A. [PRSG-BATAN, Tangerang (Indonesia)

    1998-07-01

    As part of an evaluation of the practical and economic viability of using the CANDESAL (CANDU DESALINATION) approach to desalinate water in an Indonesian environment, radiation doses to members of the public were conservatively calculated. The calculations show there is a negligible radiological impact on the public. Conservative radiation doses to members of the critical group (an adult male and a 1-year-old infant) were calculated, according to the CAN/CSA N288.1-M87 compartmental pathways analysis methodology, and show that use of the desalinated water supply would increase the dose to a member of the critical group (adult or infant) by less than 20 {mu}Sv{center_dot}a{sup -1}. About 99% of the dose from the CANDESAL facility is from tritiated heavy water (DTO or HTO) and the rest is from trace concentrations of beta and gamma emitters. The doses to a member of the critical group from a combined CANDU 6 and CANDESAL Co-generation Facility will be less than 4% of the ICRP-60 recommended effective dose limit of 1000 {mu}Sv{center_dot}a{sup -1} to a member of the public. These doses are not only a small fraction of the regulatory dose limits, but are also within the normal variations of natural background radiation levels. (author)

  3. A simulation model for Carson Ice Co-Generation Facility

    Energy Technology Data Exchange (ETDEWEB)

    Lee, N.K.W.; Elmasri, M. [Thermoflow, Inc., Wellesley, MA (United States); Brownell, G. [SMUD, Sacramento, CA (United States)

    1995-12-31

    The paper describes a software system to simulate the performance of the Carson Ice Co-gen Facility operated by the Carson Energy Group. This 100 MW plant consists of a cogeneration combined cycle and a simple cycle unit powered by LM6000 gas turbine generators. Features of the system include inlet heating/absorption chilling for the gas turbines, supplementary firing capability, and a broad range of steam turbine extractions and admissions. The software enables the operator to model complex operating scenarios. It predicts technical and economic performance under a wide range of conditions, taking into account various equipment constraints and operation preferences. For any set of user-specified operating inputs, the corresponding heat and mass balance diagrams as well as economic figures may be viewed virtually instantaneously. Interactive plots of plant heat rate, incremental heat rate, operating income, and other parameters reveal issues and trade-offs involved in performance and economic optimization.

  4. Conceptual Design of an Antiproton Generation and Storage Facility

    Energy Technology Data Exchange (ETDEWEB)

    Peggs, Stephen

    2006-10-24

    The Antiproton Generation and Storage Facility (AGSF) creates copious quantities of antiprotons, for bottling and transportation to remote cancer therapy centers. The first step in the generation and storage process is to accelerate an intense proton beam down the Main Linac for injection into the Main Ring, which is a Rapid Cycling Synchrotron that accelerates the protons to high energy. The beam is then extracted from the ring into a transfer line and into a Proton Target. Immediately downstream of the target is an Antiproton Collector that captures some of the antiprotons and focuses them into a beam that is transported sequentially into two antiproton rings. The Precooler ring rapidly manipulates antiproton bunches from short and broad (in momentum) to long and thin. It then performs some preliminary beam cooling, in the fraction of a second before the next proton bunch is extracted from the Main Ring. Pre-cooled antiprotons are passed on to the Accumulator ring before the next antiprotons arrive from the target. The Accumulator ring cools the antiprotons, compressing them into a dense state that is convenient for mass storage over many hours. Occasionally the Accumulator ring decelerates a large number of antiprotons, injecting them into a Deceleration Linac that passes them into a waiting Penning trap.

  5. Thermal vacuum life test facility for radioisotope thermoelectric generators

    Energy Technology Data Exchange (ETDEWEB)

    Deaton, R.L.; Goebel, C.J.; Amos, W.R.

    1990-01-01

    In the late 1970's, the Department of Energy (DOE) assigned Monsanto Research Corporation, Mound Facility, now operated by EG G Mound Applied Technologies, the responsibility for assembling and testing General Purpose Heat Source (GPHS) radioisotope thermoelectric generators (RTGs). Assembled and tested were five RTGs, which included four flight units and one non-flight qualification unit. Figure 1 shows the RTG, which was designed by General Electric AstroSpace Division (GE/ASD) to produce 285 W of electrical power. A detailed description of the processes for RTG assembly and testing is presented by Amos and Goebel (1989). The RTG performance data are described by Bennett, et al. (1986). The flight units will provide electrical power for the National Aeronautics and Space Administration's (NASA) Galileo mission to Jupiter (two RTGs) and the joint NASA/European Space Agency (ESA) Ulysses mission to study the polar regions of the sun (one RTG). The remaining flight unit will serve as the spare for both missions, and a non-flight qualification unit was assembled and tested to ensure that performance criteria were adequately met. 4 refs., 3 figs.

  6. Thermal vacuum life test facility for radioisotope thermoelectric generators

    Science.gov (United States)

    Deaton, R. L.; Goebel, C. J.; Amos, W. R.

    In the late 1970's, the Department of Energy (DOE) assigned Monsanto Research Corporation, Mound Facility, now operated by EG and G Mound Applied Technologies, the responsibility for assembling and testing General Purpose Heat Source (GPHS) radioisotope thermoelectric generators (RTGs). Assembled and tested were five RTGs, which included four flight units and one non-flight qualification unit. Figure 1 shows the RTG, which was designed by General Electric AstroSpace Division (GE/ASD) to produce 285 W of electrical power. A detailed description of the processes for RTG assembly and testing is presented by Amos and Goebel (1989). The RTG performance data are described by Bennett, et al., (1986). The flight units will provide electrical power for the National Aeronautics and Space Administration's (NASA) Galileo mission to Jupiter (two RTGs) and the joint NASA/European Space Agency (ESA) Ulysses mission to study the polar regions of the sun (one RTG). The remaining flight unit will serve as the spare for both missions, and a non-flight qualification unit was assembled and tested to ensure that performance criteria were adequately met.

  7. Generation of isolated asymmetric umbilics in light's polarization

    CERN Document Server

    Galvez, Enrique J; Kumar, Vijay; Viswanathan, Nirmal K

    2014-01-01

    Polarization-singularity C-points, a form of line singularities, are the vectorial counterparts of the optical vortices of spatial modes and fundamental optical features of polarization-spatial modes. Their generation in tailored beams has been limited to lemon and star C-points that contain symmetric dislocations in state-of-polarization patterns. In this article we present the theory and laboratory measurements of two complementary methods to generate isolated asymmetric C-points in tailored beams, of which symmetric lemons and stars are limiting cases; and we report on the generation of monstars, an asymmetric C-point with characteristics of both lemons and stars.

  8. In-Source Laser Resonance Ionization at ISOL Facilities

    CERN Document Server

    Marsh, Bruce; Feddosseev, Valentin

    Resonance ionization laser ion source development has been carried out at two radioactive ion beam facilities: ISOLDE (CERN, Switzerland) and the IGISOL facility (Jyvaskyla, Finland). The scope of the Resonance Ionization Laser Ion Source has been extended to 27 elements with the development of new three-step ionization schemes for Sb, Sc, Dy, Y and Au. The efficiencies were determined to be in the range of 2 - 20 %. Additionally, a new two-step ionization scheme has been developed for bismuth in an off-line atomic beam unit. The scheme relies on ionization via a strong and broad auto-ionizing resonance at an energy of 63196.79 cm$^{−1}$. This scheme may offer an improvement over the existing RILIS efficiency and will be more convenient for use during resonance ionization spectroscopy of Bi isotopes. The RILIS can be used as a spectroscopic tool to probe features such as the hyperfine structures and the isotope-shifts of radioisotopes with low production rates. By coupling a laser scanning process that dire...

  9. 76 FR 72717 - Draft Environmental Impact Statement for the Proposed KRoad Moapa Solar Generation Facility...

    Science.gov (United States)

    2011-11-25

    ... Generation Facility, Clark County, NV AGENCY: Bureau of Indian Affairs, Interior. ACTION: Notice of availability. SUMMARY: This notice advises the public that the Bureau of Indian Affairs (BIA) as the lead... Generation Facility on the Moapa River Indian Reservation (Reservation) in Clark County, Nevada. This...

  10. 77 FR 15794 - Final Environmental Impact Statement for the Proposed KRoad Moapa Solar Generation Facility...

    Science.gov (United States)

    2012-03-16

    ... Generation Facility, Clark County, NV AGENCY: Bureau of Indian Affairs, Interior. ACTION: Notice of Availability. SUMMARY: This notice advises the public that the Bureau of Indian Affairs (BIA) as the lead... Generation Facility on the Moapa River Indian Reservation (Reservation) in Clark County, Nevada. This...

  11. Utilization of paediatric isolation facilities in a TB-endemic setting.

    Science.gov (United States)

    Dramowski, Angela; Cotton, Mark F; Whitelaw, Andrew

    2015-01-01

    In hospital settings, patient isolation is used to limit transmission of certain pathogens (e.g. M. tuberculosis [TB], antibiotic-resistant bacteria and viruses causing respiratory and enteric infection). Data is lacking on utilization of paediatric isolation facilities in low-resource, TB-endemic settings. Prospective weekday observation of 18 paediatric isolation rooms at Tygerberg Children's Hospital, Cape Town, South Africa, was conducted between 1 May 2014 and 31 October 2014 documenting: occupancy rate; indication for isolation; duration of isolation; application of transmission-based precautions and infection prevention (IPC) behaviour of personnel. Potential under-utilization of isolation rooms was determined by cross-referencing isolation room occupancy with laboratory isolates of antibiotic-resistant bacteria, M. tuberculosis and selected viral pathogens. Six percent (335/5906) of hospitalized children were isolated: 78 % (260/335) for IPC purposes. Most IPC-isolated patients had community-acquired infections (213/260; 82 %), including tuberculosis (130/260; 50 %) and suspected viral infections (75/260; 29 %). Children (median age 17 months [IQR 6-50]) spent 4 days (IQR 2-8) in isolation. Isolation occupancy was 66 % (2172/3294 occupied bed days), but varied significantly by month. Laboratory data identified an additional 135 patients warranting isolation with 2054 extra bed-days required. Forty patients with 171 patient days of inappropriate isolation were identified. During 1223 weekday visits to IPC-isolated patient rooms: alcohol-based handrub was available (89 %); transmission-based precautions were appropriately implemented (71 %); and personal protective equipment was provided (74 %). Of 358 observed interactions between paediatric staff and isolated patients, hand hygiene compliance was 65 % and adherence to transmission-based precautions was 58 %. Patients isolated for TB (under airborne precautions) accounted for more than half of

  12. Development of the ultra high efficiency thermal power generation facility

    Energy Technology Data Exchange (ETDEWEB)

    Sano, Toshihiro

    2010-09-15

    In order to prevent global warming, attention is focused on nuclear power generation and renewable energy such as wind and solar power generation. The electric power suppliers of Japan are aiming to increase the amount of nuclear and non-fossil fuel power generation over 50% of the total power generation by 2020. But this means that the remaining half will still be of thermal power generation using fossil fuel and will still play an important role. Under such circumstances, further efficiency improvement of the thermal power generation and its aggressive implementation is ongoing in Japan.

  13. A Theoretical Strategy to Generate an Isolated 80-Attosecond Pulse

    Institute of Scientific and Technical Information of China (English)

    GUO Fu-Ming; YANG Yu-Jun; JIN Ming-Xing; DING Da-Jun; ZHU Qi-Ren

    2009-01-01

    Using a linearly polarized, phase-stabilized 2.66-femtcsecond driving pulse of 400 nm central wavelength orthogonally combined with another linearly polarized long pulse of 800nm central wavelength irradiating jointly on the helium atom, we demonstrate theoretically the generation of a clean isolated 80-attosecond pulse in the spectral region of 93-155 electron volts in a two-dimensional model.

  14. Iranian Light Source Facility, A third generation light source laboratory

    Directory of Open Access Journals (Sweden)

    J Rahighi

    2015-09-01

    Full Text Available The Iranian Light Source Facility (ILSF project is the first large scale accelerator facility which is currently under planning in Iran. On the basis of the present design, circumference of the 3 GeV storage ring is 528 m. Beam current and natural beam emittance are 400 mA and 0.477 nm.rad, respectively. Some prototype accelerator components such as high power solid state radio frequency amplifiers, low level RF system, thermionic RF gun, H-type dipole and quadruple magnets, magnetic measurement laboratory and highly stable magnet power supplies have been constructed at ILSF R&D laboratory

  15. Applicability of base-isolation R and D in nonreactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.; Chang, Y.W. (Argonne National Lab., IL (United States). Reactor Analysis and Safety Div.)

    1990-01-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. While the fundamental principles and technology are applicable to all of these facilities, the degree of assurance that the actual behavior of the isolation systems is as specified varies with the nature of the facility involved. Obviously, the level of effort to provide such assurance for a nuclear power plant will be much greater than that required for, say, a critical computer facility. This paper reviews the R and D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R and D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R and D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant.

  16. ISOL science at the Holifield Radioactive Ion Beam Facility

    Science.gov (United States)

    Beene, J. R.; Bardayan, D. W.; Galindo Uribarri, A.; Gross, C. J.; Jones, K. L.; Liang, J. F.; Nazarewicz, W.; Stracener, D. W.; Tatum, B. A.; Varner, R. L.

    2011-02-01

    The Holifield Radioactive Ion Beam Facility (HRIBF) provides high-quality Isotope Separator Online beams of short-lived, radioactive nuclei for nuclear structure and reaction studies, astrophysics research, and interdisciplinary applications. The primary driver, the Oak Ridge Isochronous Cyclotron, produces rare isotopes by bombarding highly refractory targets with light ions. The radioactive isotopes are ionized, formed into a beam, mass selected, injected into the 25 MV tandem, accelerated, and used in experiments. This paper reviews the HRIBF and its users' science. Note that this manuscript has been authored by UT-Battelle, LLC, under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up irrevocable, world-wide license to publish or reproduce the published form of the manuscript, or allow others to do so, for United States Government purposes.

  17. A Thermoelectric Generator Using Porous Si Thermal Isolation

    Directory of Open Access Journals (Sweden)

    Emmanouel Hourdakis

    2013-10-01

    Full Text Available In this paper we report on a thermoelectric generator (TEG using thermal isolation provided by a thick porous Si layer locally formed on the Si wafer and thermocouples composed of p-doped polycrystalline Si/Al. The “hot” contacts of the thermocouples lie on the porous Si layer, while the “cold” contacts lie on bulk crystalline Si. A housing was also designed and fabricated in order to transfer any external temperature change on the “hot” contacts of the thermocouples, the “cold” contacts being isolated from the “hot” contacts by a thick resist layer. The fabrication of the sensing element (Si die is fully compatible with batch Si processing. The output power of the thermoelectric generator depends on the porous Si isolation layer thickness, porosity, structure and morphology. For a mesoporous Si layer of 60% porosity and a macroscopic temperature differential of 10 K, an output power of 0.39 μW/cm2 was measured for a 50 μm thick porous Si layer.

  18. 42 CFR 71.47 - Special provisions relating to airports: Office and isolation facilities.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Special provisions relating to airports: Office and.... Ports: Sanitary Inspection § 71.47 Special provisions relating to airports: Office and isolation facilities. Each U.S. airport which receives international traffic shall provide without cost to...

  19. Isolation and Characterization of Rhamnolipid-Producing Bacterial Strains from a Biodiesel Facility

    Science.gov (United States)

    Novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. Strains were identified as Acinetobacter calcoaceticus, Enterobacter asburiae, E. hormaecheii, Pantoea stewartii and Pseudomona...

  20. 76 FR 61735 - Incidental Take Permit; Auwahi Wind Energy Generation Facility, Maui, HI; Draft Habitat...

    Science.gov (United States)

    2011-10-05

    ... Fish and Wildlife Service Incidental Take Permit; Auwahi Wind Energy Generation Facility, Maui, HI..., Interior. ACTION: Notice of availability; receipt of permit application. SUMMARY: Auwahi Wind Energy LLC... Hawaiian goose occurs in the vicinity of the proposed wind energy facility and may collide with...

  1. THERAPEUTIC IMPORTANCE OF OLDER GENERATION ANTIBIOTICS ON GRAM NEGATIVE ISOLATES

    Directory of Open Access Journals (Sweden)

    Lakshmi

    2015-12-01

    Full Text Available Drug resistance is a serious medical problem. Indiscriminate use of antibiotics has led to a state where multi-drug resistant bacteria have become increasingly prevalent. Therefore regular surveillance of important pathogens and their resistant pattern is mandatory. AIM To find out prevalence of organisms causing infection and their sensitivity pattern. MATERIAL AND METHODS 676 clinical samples were screened, among which 156 Gram Negative (GN Isolates were processed for their antibiotic sensitivity profile against 12 different antibiotics. RESULTS Escherichia coli is the most common isolate of 156 gram negative isolates. Among all antibiotics, Ampicillin is least sensitive (22%. Antibiotics with good sensitivity are Imipenem, Meropenem (100%, Levofloxacin 94%, Amikacin 89%, Ciprofloxacin 79%, Gentamicin 77%. Pseudomonas is 100% sensitive to Amikacin. CONCLUSION Antibiotic resistance in our area is still moderate. It is essential to test for older generation antibiotics before deciding on higher antibiotics for treatment, which will have a tremendous impact on the treatment as well as cost effectiveness. Regular surveillance helps in implementing better therapeutic strategies.

  2. THERAPEUTIC IMPORTANCE OF OLDER GENERATION ANTIBIOTICS ON GRAM NEGATIVE ISOLATES

    Directory of Open Access Journals (Sweden)

    B.Lakshmi, M.Swarajya Lakshmi, Rohini

    2015-10-01

    Full Text Available Introduction: Drug resistance is a serious medical problem. Indiscriminate use of antibiotics has led to a state where multi drug resistant bacteria have become increasingly prevalent. Therefore regular surveillance of important pathogens and their resistant pattern is mandatory. Aim: To find out prevalence of organisms causing infection and their sensitivity pattern. Material and methods: 676 clinical samples were screened among which 156 Gram Negative(GN Isolates were processed for their antibiotic sensitivity profile against 12 different antibiotics. Results: Escherichia coli is the most common isolate of 156 gram negative isolates. Among all antibiotics, ampicillin is least sensitive (22%. Antibiotics with good sensitivity are Imipenam, Meropenam (100%, Levofloxacin 94%, Amikacin 89% Ciprofloxacin 79%, Gentamycin 77%. Pseudomonas is 100% sensitive to Amikacin. Conclusion: Antibiotic resistance in our area is still moderate. It is essential to test for older generation antibiotics before deciding on higher antibiotics for treatment which will have a tremendous impact on the treatment as well as cost effectiveness. Regular surveillance helps in implementing better therapeutic strategies.

  3. Modeling HEDLA magnetic field generation experiments on laser facilities

    Science.gov (United States)

    Fatenejad, M.; Bell, A. R.; Benuzzi-Mounaix, A.; Crowston, R.; Drake, R. P.; Flocke, N.; Gregori, G.; Koenig, M.; Krauland, C.; Lamb, D.; Lee, D.; Marques, J. R.; Meinecke, J.; Miniati, F.; Murphy, C. D.; Park, H.-S.; Pelka, A.; Ravasio, A.; Remington, B.; Reville, B.; Scopatz, A.; Tzeferacos, P.; Weide, K.; Woolsey, N.; Young, R.; Yurchak, R.

    2013-03-01

    The Flash Center is engaged in a collaboration to simulate laser driven experiments aimed at understanding the generation and amplification of cosmological magnetic fields using the FLASH code. In these experiments a laser illuminates a solid plastic or graphite target launching an asymmetric blast wave into a chamber which contains either Helium or Argon at millibar pressures. Induction coils placed several centimeters away from the target detect large scale magnetic fields on the order of tens to hundreds of Gauss. The time dependence of the magnetic field is consistent with generation via the Biermann battery mechanism near the blast wave. Attempts to perform simulations of these experiments using the FLASH code have uncovered previously unreported numerical difficulties in modeling the Biermann battery mechanism near shock waves which can lead to the production of large non-physical magnetic fields. We report on these difficulties and offer a potential solution.

  4. Antibiotic resistance among cultured bacterial isolates from bioethanol fermentation facilities across the United States.

    Science.gov (United States)

    Murphree, Colin A; Heist, E Patrick; Moe, Luke A

    2014-09-01

    Bacterial contamination of fuel ethanol fermentations by lactic acid bacteria (LAB) can have crippling effects on bioethanol production. Producers have had success controlling bacterial growth through prophylactic addition of antibiotics to fermentors, yet concerns have arisen about antibiotic resistance among the LAB. Here, we report on mechanisms used by 32 LAB isolates from eight different US bioethanol facilities to persist under conditions of antibiotic stress. Minimum inhibitory concentration assays with penicillin, erythromycin, and virginiamycin revealed broad resistance to each of the antibiotics as well as high levels of resistance to individual antibiotics. Phenotypic assays revealed that antibiotic inactivation mechanisms contributed to the high levels of individual resistances among the isolates, especially to erythromycin and virginiamycin, yet none of the isolates appeared to use a β-lactamase. Biofilm formation was noted among the majority of the isolates and may contribute to persistence under low levels of antibiotics. Nearly all of the isolates carried at least one canonical antibiotic resistance gene and many carried more than one. The erythromycin ribosomal methyltransferase (erm) gene class was found in 19 of 32 isolates, yet a number of these isolates exhibit little to no resistance to erythromycin. The erm genes were present in 15 isolates that encoded more than one antibiotic resistance mechanism, suggestive of potential genetic linkages.

  5. Antimicrobial resistance and presence of the SXT mobile element in Vibrio spp. isolated from aquaculture facilities.

    Science.gov (United States)

    García-Aljaro, Cristina; Riera-Heredia, Jordi; Blanch, Anicet R

    2014-07-01

    The aim of this work was to assess the susceptibility of Vibrio spp. strains isolated from fish cultures against some usually applied antibiotics and the occurrence of the SXT mobile genetic element among them. Antimicrobial resistance was assessed by the standard disk diffusion technique while the presence of the SXT mobile genetic element was determined by conventional PCR. High levels of resistance to ampicillin (70%), cefoxitin (44%), streptomycin (31%), aztreonam (25%) and sulfamethoxazole (21%) were detected, and a high inter-and-intraspecies diversity in the resistance profile was observed for the majority of the analysed isolates. The SXT mobile genetic element was detected in only 4 isolates belonging to the species V. diazotrophicus (1), V. mediterranei (2) and V. vulnificus (1), which showed a variable antibiotic resistance profile. Horizontal antibiotic resistance gene transfer from the V. diazotrophicus SXT-positive strain to a laboratory E. coli strain was demonstrated under laboratory conditions. Our results suggest that the Vibrio spp. isolated from aquaculture facilities analysed in this study, although not being pathogenic, they constitute a source of antimicrobial resistance genes that could be mobilized to other bacterial populations through mobile genetic elements. However, the low occurrence of the SXT element in these isolates supports the hypothesis that this element is not involved in the development of resistance in the majority of Vibrio spp. in the examined aquaculture facilities.

  6. 78 FR 16302 - Crystal River Unit 3 Nuclear Generating Plant, Application for Amendment to Facility Operating...

    Science.gov (United States)

    2013-03-14

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Crystal River Unit 3 Nuclear Generating Plant, Application for Amendment to Facility Operating... Operating License No. DPR-72 for the Crystal River Unit 3 Nuclear Generating Plant (CR-3), located in...

  7. Native American Technical Assistance and Training for Renewable Energy Resource Development and Electrical Generation Facilities Management

    Energy Technology Data Exchange (ETDEWEB)

    A. David Lester

    2008-10-17

    The Council of Energy Resource Tribes (CERT) will facilitate technical expertise and training of Native Americans in renewable energy resource development for electrical generation facilities, and distributed generation options contributing to feasibility studies, strategic planning and visioning. CERT will also provide information to Tribes on energy efficiency and energy management techniques.This project will provide facilitation and coordination of expertise from government agencies and private industries to interact with Native Americans in ways that will result in renewable energy resource development, energy efficiency program development, and electrical generation facilities management by Tribal entities. The intent of this cooperative agreement is to help build capacity within the Tribes to manage these important resources.

  8. Native American Technical Assistance and Training for Renewable Energy Resource Development and Electrical Generation Facilities Management

    Energy Technology Data Exchange (ETDEWEB)

    A. David Lester

    2008-10-17

    The Council of Energy Resource Tribes (CERT) will facilitate technical expertise and training of Native Americans in renewable energy resource development for electrical generation facilities, and distributed generation options contributing to feasibility studies, strategic planning and visioning. CERT will also provide information to Tribes on energy efficiency and energy management techniques.This project will provide facilitation and coordination of expertise from government agencies and private industries to interact with Native Americans in ways that will result in renewable energy resource development, energy efficiency program development, and electrical generation facilities management by Tribal entities. The intent of this cooperative agreement is to help build capacity within the Tribes to manage these important resources.

  9. A Next Generation Light Source Facility at LBNL

    Energy Technology Data Exchange (ETDEWEB)

    Corlett, J.N.; Austin, B.; Baptiste, K.M.; Byrd, J.M.; Denes, P.; Donahue, R.; Doolittle, L.; Falcone, R.W.; Filippetto, D.; Fournier, S.; Li, D.; Padmore, H.A.; Papadopoulos, C.; Pappas, C.; Penn, G.; Placidi, M.; Prestemon, S.; Prosnitz, D.; Qiang, J.; Ratti, A.; Reinsch, M.; Sannibale, F.; Schlueter, R.; Schoenlein, R.W.; Staples, J.W.; Vecchione, T.; Venturini, M.; Wells, R.; Wilcox, R.; Wurtele, J.; Charman, A.; Kur, E.; Zholents, A.A.

    2011-03-23

    The Next Generation Light Source (NGLS) is a design concept, under development at LBNL, for a multibeamline soft x-ray FEL array powered by a ~;;2 GeV superconducting linear accelerator, operating with a 1 MHz bunch repetition rate. The CW superconducting linear accelerator is supplied by a high-brightness, highrepetition- rate photocathode electron gun. Electron bunches are distributed from the linac to the array of independently configurable FEL beamlines with nominal bunch rates up to 100 kHz in each FEL, and with even pulse spacing. Individual FELs may be configured for EEHG, HGHG, SASE, or oscillator mode of operation, and will produce high peak and average brightness x-rays with a flexible pulse format, with pulse durations ranging from sub-femtoseconds to hundreds of femtoseconds.

  10. An airborne actinobacteria Nocardiopsis alba isolated from bioaerosol of a mushroom compost facility.

    Science.gov (United States)

    Paściak, Mariola; Pawlik, Krzysztof; Gamian, Andrzej; Szponar, Bogumiła; Skóra, Justyna; Gutarowska, Beata

    2014-01-01

    Actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. Health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (MCF). The studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. Culture analysis of compost bioaerosols collected in different location of the MCF was performed. An assessment of the indoor microbial exposure revealed bacterial flora of bioaerosol in the mushroom compost facility represented by Bacillus, Geobacillus, Micrococcus, Pseudomonas, Staphylococcus spp., and actinobacterial strain with white aerial mycelium. The thermotolerant actinobacterial strain of the same morphology was repeatedly isolated from many locations in MCF: air, compost sample, and solid surface in production hall. On the base of complex morphological, chemotaxonomic, and phylogenetic characteristics, the isolate has been classified as Nocardiopsis alba. Dominant position of N. alba in microbial environment of the mushroom compost facility may represent an indicator microorganism in compost bioaerosol. The bioavailability of N. alba in mushroom compost facility creates potential risk for the health of workers, and the protection of respiratory tract and/or skin is strongly recommended.

  11. Multidrug resistant bacteria isolated from cockroaches in long-term care facilities and nursing homes.

    Science.gov (United States)

    Pai, Hsiu-Hua

    2013-01-01

    Residents in long-term care facilities and nursing homes have a relative higher risk for infections. The nocturnal and filthy habits of cockroaches may be ideal disseminators of pathogenic microorganisms in these institutions. This study was designed to determine the infestation and vector potential of cockroaches under this institutional environment. Cockroaches were collected from 69 long-term care facilities and nursing homes in Kaohsiung City. Risk factors related to cockroach infestation were determined by questionnaire survey. In addition, bacteria were isolated and identified from the alimentary tract and external surface of these insects. Antibiotic resistances of these microorganisms were then determined. Cockroach infestation was found in 45 (65.2%) institutions and 558 cockroaches (119 Periplaneta americana and 439 Blattella germanica) were collected. A significant association was found between cockroach infestation and indoor environmental sanitation. From 250 adult cockroaches, 38 species of gram-negative bacteria, 20 species of glucose non-fermenter bacilli and 6 species of gram-positive bacteria were isolated. Moreover, antibiotic resistances were found among the bacteria isolated. These findings indicate that cockroaches have the potential in transmitting pathogenic bacteria with multidrug resistances in long-term care facilities and nursing homes. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Impacts of ramping inflexibility of conventional generators on strategic operation of energy storage facilities

    DEFF Research Database (Denmark)

    Nasrolahpour, Ehsan; Kazempour, Jalal; Zareipour, Hamidreza

    2016-01-01

    This paper proposes an approach to assist a pricemaker merchant energy storage facility in making its optimal operation decisions. The facility operates in a pool-based electricity market, where the ramping capability of other resources is limited. Also, wind power resources exist in the system....... The merchant facility seeks to maximize its profit through strategic inter-temporal arbitrage decisions, when taking advantage of those ramp limitations. The market operator, on the other hand, aims at maximizing the social welfare under wind power generation uncertainty. Thus, a stochastic bi......-level optimization model is proposed, taking into account the interactions between the storage facility and the market operator, and the existing market opportunities for the storage facility. The proposed bilevel model is then transformed into a Mathematical Program with Equilibrium Constraints (MPEC) that can...

  13. Proceedings of the first international seminar on seismic base isolation for nuclear power facilities

    Energy Technology Data Exchange (ETDEWEB)

    1989-01-01

    The First International Seminar on Seismic Base Isolation of Nuclear Power Facilities was organized by the authors of this paper. It was held in San Francisco, California, USA, on August 21--22, 1989, in conjunction with the tenth International Conference on Structural Mechanics in Reactor Technology (SMiRT-10). The purpose of the seminar was to provide an international forum for discussion on the application of base isolation to nuclear power plants and of its effectiveness in reducing seismic loads and permitting standard plant designs. It also provided an opportunity for technical interchange between base isolation system designers, structural engineers, and nuclear power plant engineers. Seismic isolation is certainly one of the most significant earthquake engineering developments in recent years. This was clearly demonstrated by the very large attendance at this seminar and the various papers presented. Isolation system act as filters that reduce the seismic forces and increase the ability of isolated structures and their contents to withstand the damaging effects of earthquake motions. Each individual paper has been cataloged separately.

  14. DISTRIBUTION OF LEGIONELLA PNEUMOPHILA SEROGROUPS ISOLATED FROM WATER SYSTEMS OF PUBLIC FACILITIES IN BUSAN, SOUTH KOREA.

    Science.gov (United States)

    Hwang, In-Yeong; Park, Eun-Hee; Park, Yon-Koung; Park, Sun-Hee; Sung, Gyung-Hye; Park, Hye-Young; Lee, Young-Choon

    2016-05-01

    Legionella pneumophila is the major causes of legionellosis worldwide. The distribution of L. pneumophila was investigated in water systems of public facilities in Busan, South Korea during 2007 and 2013-2014. L. pneumophila was isolated from 8.3% of 3,055 samples, of which the highest isolation rate (49%) was from ships and the lowest 4% from fountains. Serogroups of L. pneumophila isolated in 2007 were distributed among serogroups (sgs) 1-7 with the exception of sg 4, while those of isolates during 2013 and 2014 included also 11 sgs ( 1, 2, 3, 4, 5, 6, 7, 8, 12, 13, 15). L. pneumophila sg 1 was predominated among isolates from fountains (75%), hotels (60%), buildings (44%), hospitals (38%), and public baths (37%), whereas sg 3 and sg 7 was the most prevalent from ships (46%) and factories (40%), respectively. The predominated serogroup of L. pneumophila isolates from hot and cooling tower water was sg 1 (35% and 46%, respectively), while from cold water was sg 3 (29%). These results should be useful for epidemiological surveys to identify sources of outbreaks of legionellosis in Busan, South Korea.

  15. Isolation facilities for highly infectious diseases in Europe--a cross-sectional analysis in 16 countries.

    Directory of Open Access Journals (Sweden)

    Stefan Schilling

    Full Text Available BACKGROUND: Highly Infectious Diseases (HIDs are (i easily transmissible form person to person; (ii cause a life-threatening illness with no or few treatment options; and (iii pose a threat for both personnel and the public. Hence, even suspected HID cases should be managed in specialised facilities minimizing infection risks but allowing state-of-the-art critical care. Consensus statements on the operational management of isolation facilities have been published recently. The study presented was set up to compare the operational management, resources, and technical equipment among European isolation facilities. Due to differences in geography, population density, and national response plans it was hypothesized that adherence to recommendations will vary. METHODS AND FINDINGS: Until mid of 2010 the European Network for Highly Infectious Diseases conducted a cross-sectional analysis of isolation facilities in Europe, recruiting 48 isolation facilities in 16 countries. Three checklists were disseminated, assessing 44 items and 148 specific questions. The median feedback rate for specific questions was 97.9% (n = 47/48 (range: n = 7/48 (14.6% to n = 48/48 (100%. Although all facilities enrolled were nominated specialised facilities' serving countries or regions, their design, equipment and personnel management varied. Eighteen facilities fulfilled the definition of a High Level Isolation Unit'. In contrast, 24 facilities could not operate independently from their co-located hospital, and five could not ensure access to equipment essential for infection control. Data presented are not representative for the EU in general, as only 16/27 (59.3% of all Member States agreed to participate. Another limitation of this study is the time elapsed between data collection and publication; e.g. in Germany one additional facility opened in the meantime. CONCLUSION: There are disparities both within and between European countries regarding the design

  16. Wind turbine generator interaction with diesel generators on an isolated power system

    Science.gov (United States)

    Scott, G. W.; Wilreker, V. F.; Shaltens, R. K.

    1983-01-01

    The results of a dynamic interaction investigation to characterize any disturbances caused by interfacing the Mod 0A wind turbine (150 kW configuration) with the Block Island utility diesel generator grid are reported. The tests were run when only two diesel generators were on line, and attention was given to power, frequency, and voltage time profiles. The interconnected system was examined in the start-up and synchronization phase, normal shutdown and cut-out of the wind turbine, during fixed pitch generation, and during variable pitch operation. Governors were installed on the diesel generators to accommodate the presence of wind-derived electricity. The blade pitch control was set to maintain power at 150 kW or below. Power and voltage transients were insignificant during start-up and shutdown, and frequency aberrations were within the range caused by load fluctuations. It is concluded that wind turbine generation can be successfully implemented by an isolated utility, even with a significant penetration to the total grid output.

  17. The New 2nd-Generation SRF R&D Facility at Jefferson Lab: TEDF

    Energy Technology Data Exchange (ETDEWEB)

    Reece, Charles E.; Reilly, Anthony V.

    2012-09-01

    The US Department of Energy has funded a near-complete renovation of the SRF-based accelerator research and development facilities at Jefferson Lab. The project to accomplish this, the Technical and Engineering Development Facility (TEDF) Project has completed the first of two phases. An entirely new 3,100 m{sup 2} purpose-built SRF technical work facility has been constructed and was occupied in summer of 2012. All SRF work processes with the exception of cryogenic testing have been relocated into the new building. All cavity fabrication, processing, thermal treatment, chemistry, cleaning, and assembly work is collected conveniently into a new LEED-certified building. An innovatively designed 800 m2 cleanroom/chemroom suite provides long-term flexibility for support of multiple R&D and construction projects as well as continued process evolution. The characteristics of this first 2nd-generation SRF facility are described.

  18. Economic impacts of zebra mussels on drinking water treatment and electric power generation facilities.

    Science.gov (United States)

    Connelly, Nancy A; O'Neill, Charles R; Knuth, Barbara A; Brown, Tommy L

    2007-07-01

    Invasions of nonnative species such as zebra mussels can have both ecological and economic consequences. The economic impacts of zebra mussels have not been examined in detail since the mid-1990s. The purpose of this study was to quantify the annual and cumulative economic impact of zebra mussels on surface water-dependent drinking water treatment and electric power generation facilities (where previous research indicated the greatest impacts). The study time frame was from the first full year after discovery in North America (Lake St. Clair, 1989) to the present (2004); the study area was throughout the mussels' North American range. A mail survey resulted in a response rate of 31% for electric power companies and 41% for drinking water treatment plants. Telephone interviews with a sample of nonrespondents assessed nonresponse bias; only one difference was found and adjusted for. Over one-third (37%) of surveyed facilities reported finding zebra mussels in the facility and almost half (45%) have initiated preventive measures to prevent zebra mussels from entering the facility operations. Almost all surveyed facilities (91%) with zebra mussels have used control or mitigation alternatives to remove or control zebra mussels. We estimated that 36% of surveyed facilities experienced an economic impact. Expanding the sample to the population of the study area, we estimated 267 million dollars (BCa 95% CI = 161 million dollars - 467 million dollars) in total economic costs for electric generation and water treatment facilities through late 2004, since 1989. Annual costs were greater (44,000 dollars/facility) during the early years of zebra mussel infestation than in recent years (30,000 dollars). As a result of this and other factors, early predictions of the ultimate costs of the zebra mussel invasion may have been excessive.

  19. Pulse generation and preamplification for long pulse beamlines of Orion laser facility.

    Science.gov (United States)

    Hillier, David I; Winter, David N; Hopps, Nicholas W

    2010-06-01

    We describe the pulse generation, shaping, and preamplification system for the nanosecond beamlines of the Orion laser facility. The system generates shaped laser pulses of up to approximately 1 J of 100 ps-5 ns duration with a programmable temporal profile. The laser has a 30th-power supergaussian spatial profile and is diffraction limited. The system is capable of imposing 2D smoothing by spectral dispersion upon the beam, which will produce a nonuniformity of 10% rms at the target.

  20. Bulk processing of radionuclide generator parents at the Los Alamos Hot Cell Facility

    Energy Technology Data Exchange (ETDEWEB)

    Fassbender, M.; Nortier, F.M.; Phillips, D.R. [Los Almos National Laboratory, NM (US). Chemistry Div.] (and others)

    2004-07-01

    Bulk radionuclide processing at Los Alamos includes isotopes (''generator parents'') with short-lived radioactive daughter nuclides for medical applications: Germanium-68, {sup 82}Sr, {sup 88}Zr and {sup 109}Cd are regularly prepared at the Los Alamos Hot Cell Facility. Nuclear chemical aspects related to the production and processing of these generator parents are briefly outlined below. (orig.)

  1. Ultraviolet Light Generation and Transport in the Final Optics Assembly of the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Wegner, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hackel, L. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Feit, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Parham, T. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Kozlowski, M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Whitman, P. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-02-12

    The design of the National Ignition Facility (NIF) includes a Final Optics Assembly (FOA) subsystem for ultraviolet (UV) light generation and transport for each of the 192 beamlines. Analytical and experimental work has been done to help understand and predict the performance of FOA.

  2. Vibration test report on crossover piping system in seismic isolation nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Uryu, Mitsuru; Shinohara, Takaharu; Terada, Shuji; Yamazaki, Toshihiko; Tomita, Tsuneo; Kondo, Toshinari

    1999-03-01

    In a seismic isolation nuclear facility, crossover piping system is subjected to large relative displacement and inertia forces during earthquakes. Hinged bellows expansion joints are utilized for accommodation to such the large displacement. This report describes tests for validation of developed simulation code with analytical models. Seismic experiments by a vibration test machine were conducted using actual size piping system models. A comparison between test results and analytical results showed a favorable agreement. The vibration test demonstrated that the structural integrity of this piping system would be maintained during earthquakes. (H. Itami)

  3. R D activities at Argonne National Laboratory for the application of base seismic isolation in nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.

    1991-01-01

    Argonne National Laboratory (ANL) has been deeply involved in the development of seismic isolation for use in nuclear facilities for the past decade. Initial focus of these efforts has been on the use of seismic isolation for advanced liquid metal reactors (LMR). Subsequent efforts in seismic isolation at ANL included a lead role in an accelerated development program for possible use of seismic isolation for the DOE's New Production reactors (NPR). Under funding provided by the National Science Foundation (NSF) Argonne is currently working with Shimizu in a joint United States-Japanese program on response of seismically-isolated buildings to actual earthquakes. The results of recent work in the seismic isolation program elements are described in this paper. The current Status of these programs is presented along with an assessment of work still needed to bring the benefits of this emerging technology to full potential in nuclear reactors and other nuclear facilities. 38 refs., 3 figs.

  4. Use of base isolation techniques for the design of high-level waste storage facility enclosure at INEL

    Energy Technology Data Exchange (ETDEWEB)

    Vallenas, J.M.; Wong, Chun K. [Cygna Energy Services, Oakland, CA (United States); Beer, M.J. [Westinghouse Idaho Nuclear Co., Inc., Idaho Falls, ID (United States)

    1993-08-01

    Current Department of Energy criteria for facilities subjected to natural hazards provide guidelines to place facilities or portions of facilities into usage categories. Usage categories are based on characteristics such as mission dependence, type of hazardous materials involved, and performance goals. Seismic requirements are significantly more stringent for facilities falling into higher ``hazard facility use categories``. A special problem arises in cases where a facility or portion of a facility is dependent on another facility of lower ``hazard facility use category`` for support or protection. Creative solutions can minimize the cost Unpact of ensuring that the lower category item does not compromise the performance of the higher category item. In this paper, a base isolation solution is provided for a ``low hazard facility use category`` weather enclosure designed so it will not collapse onto a ``high hazard facility use category`` high level waste storage facility at INEL. This solution is compared to other more conventional procedures. Details, practical limitations, licensing and regulatory considerations, and cost comparisons are provided.

  5. Advanced Electron Beam Ion Sources (EBIS) for 2-nd generation carbon radiotherapy facilities

    CERN Document Server

    Shornikov, A.

    2016-01-01

    In this work we analyze how advanced Electron Beam Ion Sources (EBIS) can facilitate the progress of carbon therapy facilities. We will demonstrate that advanced ion sources enable operation of 2-nd generation ion beam therapy (IBT) accelerators. These new accelerator concepts with designs dedicated to IBT provide beams better suited for therapy and, are more cost efficient than contemporary IBT facilities. We will give a sort overview of the existing new IBT concepts and focus on those where ion source technology is the limiting factor. We will analyse whether this limitation can be overcome in the near future thanks to ongoing EBIS development.

  6. New developments on preparation of cooled and bunched Radioactive Ion beams at ISOL facilities the ISCOOL project and the rotating wall cooling

    CERN Document Server

    Podadera-Aliseda, I

    2006-01-01

    The future of physics research with radioactive isotopes is linked to the development of more accurate and specific experiments which will work with more exotic and energetic nuclei. Requirements from experiments are getting more and more stringent and represent a big challenge for the preparation of ion beams, since each one demands different optical specifications to optimize its setup and improve its results. This thesis focuses on the new developments achieved in preparation of RIB's (Radioactive Ion Beams) at ISOL facilities. Optical beam improvements based on cooling and bunching are the main points of the work. The design of a new general purpose second generation RFQCB (Radio Frequency ion Quadrupole Cooler and Buncher) for ISOLDE, the oldest ISOL facility, will be completely described. Nowadays, RFQCB's are one of the most important devices in the beam preparation stage thanks to the capacity to cool and bunch low-intensity ion beams quickly, easily and with great efficiency. The thesis will detail t...

  7. Optimal configuration of power generating systems in isolated island with renewable energy

    Energy Technology Data Exchange (ETDEWEB)

    Senjyu, Tomonobu; Hayashi, Daisuke; Yona, Atsushi; Urasaki, Naomitsu [Faculty of Engineering, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Nakagami, Okinawa 903-0213 (Japan); Funabashi, Toshihisa [Meidensha Corporation, 36-2 Nihonbashi Hakozakicho, Chuo-ku, Tokyo 103-8515 (Japan)

    2007-09-15

    In isolated islands, usually diesel generators supply electric power. However, there are problems, e.g., a lack of fossil fuel, environmental pollution etc. So, isolated island, e.g. Miyako island, installs renewable energy power production plants. However, renewable energy power production plants are very costly. This paper presents an optimal configuration of power system in isolated island installing renewable energy power production plants. The generating system consists of diesel generators, wind turbine generators, PV system and batteries. Using the proposed method, operation cost can be reduced about 10% in comparison with diesel generators only from simulation results. (author)

  8. Selection and evaluation of thermal criteria for a geologic waste isolation facility in salt

    Energy Technology Data Exchange (ETDEWEB)

    1976-09-01

    Previous design efforts for geologic waste isolation facilities in bedded salt developed several limiting temperature conditions, or thermal criteria, for use in parametric studies. These criteria, along with other design parameters, must assure that the temperature variations that occur do not adversely affect operating personnel and equipment during normal operations as well as assure containment and environmental integrity. The goals of the present study are to review past analyses of thermal criteria, determine the factors that should be considered in defining thermal criteria, suggest appropriate procedures for determining thermal criteria and suggest additional experimental and computational efforts required to adequately determine thermal criteria. The approach taken was to divide consideration of the system into four categories of thermal effects and four phases of facility operation. The categories were effects on the canister and its immediate environs, effects on the operating environment experienced by personnel and equipment, effects on storage room integrity, and effects on the biosphere. The phases of facility operation were loading, storage, retrieval, and disposal. Each of the categories was further subdivided into several aspects for detailed consideration of thermal effects through the four operating phases. This comprehensive approach was taken to ensure that all conceivable thermal effects were included, and to demonstrate a systematic approach for use in developing thermal criteria for other geologic media.

  9. A methodology to identify stranded generation facilities and estimate stranded costs for Louisiana's electric utility industry

    Science.gov (United States)

    Cope, Robert Frank, III

    1998-12-01

    The electric utility industry in the United States is currently experiencing a new and different type of growing pain. It is the pain of having to restructure itself into a competitive business. Many industry experts are trying to explain how the nation as a whole, as well as individual states, will implement restructuring and handle its numerous "transition problems." One significant transition problem for federal and state regulators rests with determining a utility's stranded costs. Stranded generation facilities are assets which would be uneconomic in a competitive environment or costs for assets whose regulated book value is greater than market value. At issue is the methodology which will be used to estimate stranded costs. The two primary methods are known as "Top-Down" and "Bottom-Up." The "Top-Down" approach simply determines the present value of the losses in revenue as the market price for electricity changes over a period of time into the future. The problem with this approach is that it does not take into account technical issues associated with the generation and wheeling of electricity. The "Bottom-Up" approach computes the present value of specific strandable generation facilities and compares the resulting valuations with their historical costs. It is regarded as a detailed and difficult, but more precise, approach to identifying stranded assets and their associated costs. This dissertation develops a "Bottom-Up" quantitative, optimization-based approach to electric power wheeling within the state of Louisiana. It optimally evaluates all production capabilities and coordinates the movement of bulk power through transmission interconnections of competing companies in and around the state. Sensitivity analysis to this approach is performed by varying seasonal consumer demand, electric power imports, and transmission inter-connection cost parameters. Generation facility economic dispatch and transmission interconnection bulk power transfers, specific

  10. NSR&D Program Fiscal Year (FY) 2015 Call for Proposals Mitigation of Seismic Risk at Nuclear Facilities using Seismic Isolation

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, Justin [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-02-01

    Seismic isolation (SI) has the potential to drastically reduce seismic response of structures, systems, or components (SSCs) and therefore the risk associated with large seismic events (large seismic event could be defined as the design basis earthquake (DBE) and/or the beyond design basis earthquake (BDBE) depending on the site location). This would correspond to a potential increase in nuclear safety by minimizing the structural response and thus minimizing the risk of material release during large seismic events that have uncertainty associated with their magnitude and frequency. The national consensus standard America Society of Civil Engineers (ASCE) Standard 4, Seismic Analysis of Safety Related Nuclear Structures recently incorporated language and commentary for seismically isolating a large light water reactor or similar large nuclear structure. Some potential benefits of SI are: 1) substantially decoupling the SSC from the earthquake hazard thus decreasing risk of material release during large earthquakes, 2) cost savings for the facility and/or equipment, and 3) applicability to both nuclear (current and next generation) and high hazard non-nuclear facilities. Issue: To date no one has evaluated how the benefit of seismic risk reduction reduces cost to construct a nuclear facility. Objective: Use seismic probabilistic risk assessment (SPRA) to evaluate the reduction in seismic risk and estimate potential cost savings of seismic isolation of a generic nuclear facility. This project would leverage ongoing Idaho National Laboratory (INL) activities that are developing advanced (SPRA) methods using Nonlinear Soil-Structure Interaction (NLSSI) analysis. Technical Approach: The proposed study is intended to obtain an estimate on the reduction in seismic risk and construction cost that might be achieved by seismically isolating a nuclear facility. The nuclear facility is a representative pressurized water reactor building nuclear power plant (NPP) structure

  11. Java facilities in processing XML files - JAXB and generating PDF reports

    Directory of Open Access Journals (Sweden)

    Danut-Octavian SIMION

    2008-01-01

    Full Text Available The paper presents the Java programming language facilities in working with XML files using JAXB (The Java Architecture for XML Binding technology and generating PDF reports from XML files using Java objects. The XML file can be an existing one and could contain the data about an entity (Clients for example or it might be the result of a SELECT-SQL statement. JAXB generates JAVA classes through xs rules and a Marshalling, Unmarshalling compiler. The PDF file is build from a XML file and uses XSL-FO formatting file and a Java ResultSet object.

  12. Computational design of high efficiency release targets for use at ISOL facilities

    CERN Document Server

    Liu, Y

    1999-01-01

    This report describes efforts made at the Oak Ridge National Laboratory to design high-efficiency-release targets that simultaneously incorporate the short diffusion lengths, high permeabilities, controllable temperatures, and heat-removal properties required for the generation of useful radioactive ion beam (RIB) intensities for nuclear physics and astrophysics research using the isotope separation on-line (ISOL) technique. Short diffusion lengths are achieved either by using thin fibrous target materials or by coating thin layers of selected target material onto low-density carbon fibers such as reticulated-vitreous-carbon fiber (RVCF) or carbon-bonded-carbon fiber (CBCF) to form highly permeable composite target matrices. Computational studies that simulate the generation and removal of primary beam deposited heat from target materials have been conducted to optimize the design of target/heat-sink systems for generating RIBs. The results derived from diffusion release-rate simulation studies for selected t...

  13. SENSITIVITY OF MOLDS ISOLATED FROM WAREHOUSES OF FOOD PRODUCTION FACILITY ON SELECTED ESSENTIAL OILS

    Directory of Open Access Journals (Sweden)

    Łukasz Kręcidło

    2015-07-01

    Full Text Available Storage of raw materials is one of steps in food production chain. The aim of this study was to estimate the influence of selected essential oils on the growth of four fungal strains: Trichoderma viride, Rhizomucor miehei, Penicillium chrysogenum, Penicillium janthinellum. Strains were isolated from warehouses of the food production facility. Selected essential oils: thyme oil, rosewood oil and rosemary oil were used to assess antifungal activity. Chemical composition of essential oils was determined by Gas Chromatography-Mass Spectroscopy (GC-MS. Antifungal activity of essential oils was estimated in relative to peracetic acid (PAA and sterile water with Tween 80 (0,5%. The influence of essential oils on fungal growth was carried by medium poisoning method. Increment of fungal mycelium was measured every day by 10 days. The thyme essential oils totally inhibited fungal growth in the lowest concentration of 1 mm3·cm-3. The most resistant strain was Penicillium janthinellum.

  14. Hardy Bacterium Isolated From Two Geographically Distinct Spacecraft Assembly Cleanroom Facilities

    Science.gov (United States)

    Vaisham-payan, Parag A.; Venkateswaran, Kasthuri J.; Schwendner, Petra; Moissl-Eichinger, Christine

    2012-01-01

    Earlier studies have confirmed that a tenacious hardy bacterial population manages to persist and survive throughout a spacecraft assembly process. The widespread detection of these organisms underscores the challenges in eliminating them completely. Only comprehensive and repetitive microbial diversity studies of geographically distinct cleanroom facilities will bolster the understanding of planetary protection relevant microbes. Extensive characterizations of the physiological traits demonstrated by cleanroom microbes will aid NASA in gauging the forward contamination risk that hardy bacteria (such as Tersicoccus phoenicis) pose to spacecraft. This study reports on the isolation and identification of two gram-positive, non-motile, non-spore-forming bacterial strains from the spacecraft assembly facilities at Kennedy Space Center, Florida, USA and Centre Spatial Guyanais, Kourou, French Guiana. DNA-DNA relatedness values between the novel strains indicates that these novel strains were indeed members of a same species. Phylogenetic evidence derived from a 16S ribosomal DNA analysis indicated that both the novel strains are less closely related to all other Arthrobacter species.

  15. Applicability of base-isolation R D in non-reactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W.; Chang, Y.W.

    1990-01-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. While the fundamental principles and technology are applicable to all of these facilities, the degree of assurance that the actual behavior of the isolation systems is as specified varies with the nature of the facility involved. Obviously, the level of effort to provide such assurance for a nuclear power plant will be much greater than that required for, say, a critical computer facility. The question, therefore, is to what extent can research and development (R D) for non-nuclear use be used to provide technological data needed for seismic isolation of a nuclear power plant. This question, of course is not unique to seismic isolation. Virtually every structural component, system, or piece of equipment used in nuclear power plants is also used in non- nuclear facilities. Experience shows that considerable effort is needed to adapt conventional technology into a nuclear power plant. Usually, more thorough analysis is required, material and fabrication quality-control requirements are more stringent as are controls on field installation. In addition, increased emphasis on maintainability and inservice inspection throughout the life of the plant is generally required to gain acceptance in nuclear power plant application. This paper reviews the R D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant. 2 refs.

  16. Stability Analysis for Isolated AC Microgrids Based on PV-Active Generators

    DEFF Research Database (Denmark)

    Aldana, Nelson Leonardo Diaz; Coelho, Ernane A. A.; Quintero, Juan Carlos Vasquez;

    2015-01-01

    The current trend in isolated microgrids is oriented to distributed renewable energy generators, such as photovoltaic (PV) generators and their corresponding distributed energy storage systems (ESS) as an unit denoted as active generator (PV+ESS). In an isolated microgrid, every distributed...... for all the distributed generators. In particular, ESS’s based on batteries require at least two different mode of charge. As consequence, the topological operation mode of the microgrid is affected by the changes of the operation mode of each distributed generator. Typically, droop control loops are used...... for interconnecting several different distributed generators in parallel to a common bus, whose parameters determine the stability and damping of the microgrid operation. In this paper, a small-signal stability analysis is applied to an isolated AC microgrid composed of (PV+ESS) active generators, regarding three...

  17. Applicability of base-isolation R and D in non-reactor facilities to a nuclear reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Seidensticker, R.W. (Argonne National Lab., IL (USA))

    1991-06-01

    Seismic isolation is gaining increased attention worldwide for use in a wide spectrum of critical facilities, ranging from hospitals and computing centers to nuclear power plants. The level of assurance of performance for such isolation systems for a nuclear power plant will be much greater than that required for non-nuclear facilities. The question is to what extent can R and D for non-nuclear use of seismic isolation be applied to a nuclear power plant. Experience shows that considerable effort is needed to adapt any technology to nuclear power facilities. This paper reviews the R and D programs ongoing for seismic isolation in non-nuclear facilities and related experience and makes a preliminary assessment of the extent to which such R and D and experience can be used for nuclear power plant application. Ways are suggested to improve the usefulness of such non-nuclear R and D in providing the high level of confidence required for the use of seismic isolation in a nuclear reactor plant. (orig.).

  18. Decadal Vision Progress Report Implementation Plans and Status for the Next Generation ARM Facility

    Energy Technology Data Exchange (ETDEWEB)

    Mather, James

    2016-06-01

    The reconfiguration of the ARM facility, formally initiated in early 2014, is geared toward implementing the Next Generation of the ARM Facility, which will more tightly link ARM measurements and atmospheric models. The strategy is outlined in the ARM Climate Research Facility Decadal Vision (DOE 2014a). The strategy includes the implementation of a high-resolution model, initially at the Southern Great Plains (SGP) site, and enhancements at the SGP and North Slope of Alaska (NSA) sites to provide additional observations to support modeling and process studies. Enhancements at the SGP site focus on ground-based instruments while enhancements at the NSA make use of Unmanned Aerial Systems (UAS) and Tethered Balloon Systems (TBS). It is also recognized that new data tools and data products will need to be developed to take full advantage of these improvements. This document provides an update on the status of these ARM facility enhancements, beginning with the measurement enhancements at the SGP and NSA, followed by a discussion of the modeling project including associated data-processing activities.

  19. THERAPEUTIC IMPORTANCE OF OLDER GENERATION ANTIBIOTICS ON GRAM NEGATIVE ISOLATES

    OpenAIRE

    B.Lakshmi, M.Swarajya Lakshmi, Rohini

    2015-01-01

    Introduction: Drug resistance is a serious medical problem. Indiscriminate use of antibiotics has led to a state where multi drug resistant bacteria have become increasingly prevalent. Therefore regular surveillance of important pathogens and their resistant pattern is mandatory. Aim: To find out prevalence of organisms causing infection and their sensitivity pattern. Material and methods: 676 clinical samples were screened among which 156 Gram Negative(GN) Isolates were processed for their a...

  20. THERAPEUTIC IMPORTANCE OF OLDER GENERATION ANTIBIOTICS ON GRAM NEGATIVE ISOLATES

    OpenAIRE

    Lakshmi; Swarajya Lakshmi; Rohini,

    2015-01-01

    Drug resistance is a serious medical problem. Indiscriminate use of antibiotics has led to a state where multi-drug resistant bacteria have become increasingly prevalent. Therefore regular surveillance of important pathogens and their resistant pattern is mandatory. AIM To find out prevalence of organisms causing infection and their sensitivity pattern. MATERIAL AND METHODS 676 clinical samples were screened, among which 156 Gram Negative (GN) Isolates were ...

  1. Electromagnetic Fields Associated with Commercial Solar Photovoltaic Electric Power Generating Facilities.

    Science.gov (United States)

    Tell, R A; Hooper, H C; Sias, G G; Mezei, G; Hung, P; Kavet, R

    2015-01-01

    The southwest region of the United States is expected to experience an expansion of commercial solar photovoltaic generation facilities over the next 25 years. A solar facility converts direct current generated by the solar panels to three-phase 60-Hz power that is fed to the grid. This conversion involves sequential processing of the direct current through an inverter that produces low-voltage three-phase power, which is stepped up to distribution voltage (∼12 kV) through a transformer. This study characterized magnetic and electric fields between the frequencies of 0 Hz and 3 GHz at two facilities operated by the Southern California Edison Company in Porterville, CA and San Bernardino, CA. Static magnetic fields were very small compared to exposure limits established by IEEE and ICNIRP. The highest 60-Hz magnetic fields were measured adjacent to transformers and inverters, and radiofrequency fields from 5-100 kHz were associated with the inverters. The fields measured complied in every case with IEEE controlled and ICNIRP occupational exposure limits. In all cases, electric fields were negligible compared to IEEE and ICNIRP limits across the spectrum measured and when compared to the FCC limits (≥0.3 MHz).

  2. Integrated doses calculation in evacuation scenarios of the neutron generator facility at Missouri S&T

    Science.gov (United States)

    Sharma, Manish K.; Alajo, Ayodeji B.

    2016-08-01

    Any source of ionizing radiations could lead to considerable dose acquisition to individuals in a nuclear facility. Evacuation may be required when elevated levels of radiation is detected within a facility. In this situation, individuals are more likely to take the closest exit. This may not be the most expedient decision as it may lead to higher dose acquisition. The strategy followed in preventing large dose acquisitions should be predicated on the path that offers least dose acquisition. In this work, the neutron generator facility at Missouri University of Science and Technology was analyzed. The Monte Carlo N-Particle (MCNP) radiation transport code was used to model the entire floor of the generator's building. The simulated dose rates in the hallways were used to estimate the integrated doses for different paths leading to exits. It was shown that shortest path did not always lead to minimum dose acquisition and the approach was successful in predicting the expedient path as opposed to the approach of taking the nearest exit.

  3. Integrated doses calculation in evacuation scenarios of the neutron generator facility at Missouri S&T

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Manish K.; Alajo, Ayodeji B., E-mail: alajoa@mst.edu

    2016-08-11

    Any source of ionizing radiations could lead to considerable dose acquisition to individuals in a nuclear facility. Evacuation may be required when elevated levels of radiation is detected within a facility. In this situation, individuals are more likely to take the closest exit. This may not be the most expedient decision as it may lead to higher dose acquisition. The strategy followed in preventing large dose acquisitions should be predicated on the path that offers least dose acquisition. In this work, the neutron generator facility at Missouri University of Science and Technology was analyzed. The Monte Carlo N-Particle (MCNP) radiation transport code was used to model the entire floor of the generator's building. The simulated dose rates in the hallways were used to estimate the integrated doses for different paths leading to exits. It was shown that shortest path did not always lead to minimum dose acquisition and the approach was successful in predicting the expedient path as opposed to the approach of taking the nearest exit.

  4. Physics and Technology for the Next Generation of Radioactive Ion Beam Facilities: EURISOL

    CERN Document Server

    Kadi, Y; Catherall, R; Giles, T; Stora, T; Wenander, F K

    2012-01-01

    Since the discovery of artificial radioactivity in 1935, nuclear scientists have developed tools to study nuclei far from stability. A major breakthrough came in the eighties when the first high energy radioactive beams were produced at Berkeley, leading to the discovery of neutron halos. The field of nuclear structure received a new impetus, and the major accelerator facilities worldwide rivalled in ingenuity to produce more intense, purer and higher resolution rare isotope beams, leading to our much improved knowledge and understanding of the general evolution of nuclear properties throughout the nuclear chart. However, today, further progress is hampered by the weak beam intensities of current installations which correlate with the difficulty to reach the confines of nuclear binding where new phenomena are predicted, and where the r-process path for nuclear synthesis is expected to be located. The advancement of Radioactive Ion Beam (RIB) science calls for the development of so-called next-generation facil...

  5. Stochastic Multi-Commodity Facility Location Based on a New Scenario Generation Technique

    Science.gov (United States)

    Mahootchi, M.; Fattahi, M.; Khakbazan, E.

    2011-11-01

    This paper extends two models for stochastic multi-commodity facility location problem. The problem is formulated as two-stage stochastic programming. As a main point of this study, a new algorithm is applied to efficiently generate scenarios for uncertain correlated customers' demands. This algorithm uses Latin Hypercube Sampling (LHS) and a scenario reduction approach. The relation between customer satisfaction level and cost are considered in model I. The risk measure using Conditional Value-at-Risk (CVaR) is embedded into the optimization model II. Here, the structure of the network contains three facility layers including plants, distribution centers, and retailers. The first stage decisions are the number, locations, and the capacity of distribution centers. In the second stage, the decisions are the amount of productions, the volume of transportation between plants and customers.

  6. First generation synchrony of isolated Hyphomicrobium swarmer populations.

    Science.gov (United States)

    Moore, R L; Hirsch, P

    1973-10-01

    A method is described for obtaining synchronously growing swarmer cell populations of Hyphomicrobium sp. strain B-522. This was accomplished by isolating young swarmers from random cultures by centrifugation and filtration. Cell multiplication occurred during 38% of the growth cycle in populations synchronized in this manner. Observations were made of the changes in cellular morphology which occurred during the growth cycle. Of the 14.25 h required for the doubling in cell numbers, an average of 5 h passed before the swarmer cells began to develop their hyphae. This time varied over a range of 10 h. The time interval between the beginning of hyphal development and the beginning of bud formation was 3.5 to 4.5 h. The maturation of the first buds and their separation from the mother cells were completed in 5.5 h. The duration of these steps is compared to those measured previously in agar slide cultures.

  7. Developing Planetary Protection Technologies: Isolation and Characterization of Novel Microbes from a Spacecraft Assembly Facility

    Science.gov (United States)

    Quigley, M. S.; Kempf, M.; Venkateswaran, K.; kern, r

    2001-12-01

    Systematic detection and classification of cultivable microbes present in the Spacecraft Assembly Facility (SAF) at the Jet Propulsion Laboratory (JPL) were carried out using classical microbial phylogeny and advanced molecular microbial ecology methods. This work is being carried out to understand the microbial diversity in an assembly facility that could potentially contaminate an assembled spacecraft. Contamination of spacecraft surfaces with terrestrial microbes could compromise the interpretation of results from in-situ life detection studies or sample return missions. Fifty samples were collected from various locations of JPL-SAF whose air circulation and humidity are controlled and maintained to the cleanliness of a class 100K clean-room. Sampling locations included both unclassified (entrance floors, shoe-cleaner, air-shower, ante-room, and air-lock), and classified (clean-room floors, clean-room tables and cabinet tops) areas. All samples were analyzed for the incidence of aerobic spore-formers (using Tryptic Soy Agar) and for total aerobic heterotrophs (using R2 Agar). Spore-former incidence ranged from 0 to 3x10{-1 } CFU/25cm{2 }in the unclassified area whereas only 2 out of 25 samples collected in the unclassified areas contained spore-formers. However, the counts of total heterotrophs were about 100 times higher when compared to the spore-formers in the unclassified area and 9 out of 25 samples collected in the classified area contained a range of heterotrophs from 5x101 to 4x102 CFU/25cm2 . Representatives of the spore-formers (31 strains) and total heterotrophs (40 strains) were identified by 16S rDNA sequence analysis to determine their phylogenetic relatedness. The spore-formers clustered to 8 known Bacillus and Paenibacillus species and 6 strains were identified as novel species of the genera Bacillus (2), Paenibacillus (2), Ureibacillus (1), and one new genus. Among the forty heterotrophs, 5 clusters were tightly affiliated with genera, such as

  8. Conceptual aspects of fiscal interactions between local governments and federally-owned, high-level radioactive waste-isolation facilities

    Energy Technology Data Exchange (ETDEWEB)

    Bjornstad, D.J.; Johnson, K.E.

    1981-01-01

    This paper examines a number of ways to transfer revenues between a federally-owned high level radioactive waste isolation facility (hereafter simply, facility) and local governments. Such payments could be used to lessen fiscal disincentives or to provide fiscal incentives for communities to host waste isolation facilities. Two facility characteristics which necessitate these actions are singled out for attention. First, because the facility is federally owned, it is not liable for state and local taxes and may be viewed by communities as a fiscal liability. Several types of payment plans to correct this deficiency are examined. The major conclusion is that while removal of disincentives or creation of incentives is possible, plans based on cost compensation that fail to consider opportunity costs cannot create incentives and are likely to create disincentives. Second, communities other than that in which the facility is sited may experience costs due to the siting and may, therefore, oppose it. These costs (which also accrue to the host community) arise due to the element of risk which the public generally associates with proximity to the transport and storage of radioactive materials. It is concluded that under certain circumstances compensatory payments are possible, but that measuring these costs will pose difficulty.

  9. About technical possibility to use VEERA facility for investigation of coolant stratification phenomenon in horizontal steam generators

    Energy Technology Data Exchange (ETDEWEB)

    Mitioukov, V.; Mitrioukhine, A. [St. Petersburg State Technical Univ. (Russian Federation); Korteniemi, V. [Lappeenranta Univ. of Technology (Finland)

    1997-12-31

    The presentation gives a brief insight on possibility of using the VEERA facility in studying the stratification phenomenon. The idea for such experiments is to use the facility upper plenum part to simulate the conditions in upper part of horizontal steam generator hot collector. The upper part of steam generator hot collector is one of the locations where the stratification can take part during natural circulation mode. 4 refs.

  10. Soybean Ferritin: Isolation, Characterization, and Free Radical Generation

    Institute of Scientific and Technical Information of China (English)

    Andrea Galatro; Elizabeth Robello; Susana Puntarulo

    2012-01-01

    The main aim of this work was to assess the multi-task role of ferritin (Ft) in the oxidative metabolism of soybean (Glycine max).Soybean seeds incubated for 24 h yielded 41 ± 5 μg Ft/g fresh weight.The rate of in vitro incorporation of iron (Fe) into Ft was tested by supplementing the reaction medium with physiological Fe chelators.The control rate,observed in the presence of 100 μM Fe,was not significantly different from the values observed in the presence of 100 μM Fe-his.However,it was significantly higher in the presence of 100 μM Fe-citrate (approximately 4.5-fold) or of 100 μM Fe-ATP (approximately 14-fold).Moreover,a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate,as compared with the control.On the other hand,Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical,according to its capacity for Fe uptake.The data presented here suggest that Ft could be involved in the generation of free radicals,such as hydroxyl radical,by Fe-catalyzed reactions.Moreover,the scavenging of these radicals by Ft itself could then lead to protein damage.However,Ft could also prevent cellular damage by the uptake of catalytically active Fe.

  11. Computational Design of High Efficiency Release Targets for Use at ISOL Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Alton, G.D.; Liu, Y.; Middleton, J.W.

    1998-11-04

    This report describes efforts made at the Oak Ridge National Laboratory to design high-efficiency-release targets that simultaneously incorporate the short diffusion lengths, high permeabilities, controllable temperatures, and heat removal properties required for the generation of useful radioactive ion beam (RIB) intensities for nuclear physics and astrophysics research using the isotope separation on-line (ISOL) technique. Short diffusion lengths are achieved either by using thin fibrous target materials or by coating thin layers of selected target material onto low-density carbon fibers such as reticulated vitreous carbon fiber (RVCF) or carbon-bonded-carbon-fiber (CBCF) to form highly permeable composite target matrices. Computational studies which simulate the generation and removal of primary beam deposited heat from target materials have been conducted to optimize the design of target/heat-sink systems for generating RIBs. The results derived tlom diffusion release-rate simulation studies for selected targets and thermal analyses of temperature distributions within a prototype target/heat-sink system subjected to primary ion beam irradiation will be presented in this report.

  12. Test facility for advanced electric adjustable frequency drives and generators of typical industrial ratings. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-12-01

    A test facility has been developed, for electric adjustable-speed motors and variable-speed generators, that is unique in US universities in terms of its range (5 to 300 hp currently with 0.1 to 1,000 hp final capability) and flexibility (standard NEMA frame and novel geometry machines can be accommodated). The basic facility was constructed with funding from the Electric Power Research Institute. The instrumentation obtained under this DOE grant has been integrated into the facility which was completed in Fall 1997. The facility has already provided useful studies for DOE, EPRI, as well as several West Coast industries and electric energy utilities.

  13. Beta-Delayed Multiparticle Emission Studies at ISOL-type Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Borge, M.J.G. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Bergmann, U.C. [Institut for fysik og astronomi, Aarhus Universitet, DK-8000 Aarhus C (Denmark); EP Division, CERN, CH-1211 Geneva 23 (Switzerland); Boutami, R. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Cederkaell, J. [EP Division, CERN, CH-1211 Geneva 23 (Switzerland); Dendooven, P. [Department of Physics, University of Jyvaaeskylae, FIN-40351 Jyvaaeskylae (Finland); Fraile, L.M. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Fynbo, H.O.U. [Institut for fysik og astronomi, Aarhus Universitet, DK-8000 Aarhus C (Denmark); Huang, W.X. [Department of Physics, University of Jyvaaeskylae, FIN-40351 Jyvaaeskylae (Finland); Huikari, J. [Department of Physics, University of Jyvaaeskylae, FIN-40351 Jyvaaeskylae (Finland); Jading, Y. [EP Division, CERN, CH-1211 Geneva 23 (Switzerland); Jeppesen, H. [Institut for fysik og astronomi, Aarhus Universitet, DK-8000 Aarhus C (Denmark); Jokinen, A. [Department of Physics, University of Jyvaaeskylae, FIN-40351 Jyvaaeskylae (Finland); Jonson, B. [Experimentell Fysik, Chalmers Tekniska Hoegskola, S-41296 Goeteborg (Sweden); Martel, I. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Meister, M. [Experimentell Fysik, Chalmers Tekniska Hoegskola, S-41296 Goeteborg (Sweden); Nilsson, T. [Experimentell Fysik, Chalmers Tekniska Hoegskola, S-41296 Goeteborg (Sweden); Nyman, G. [Experimentell Fysik, Chalmers Tekniska Hoegskola, S-41296 Goeteborg (Sweden); Prezado, Y. [Instituto de Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Riisager, K. [Institut for fysik og astronomi, Aarhus Universitet, DK-8000 Aarhus C (Denmark)] [and others

    2004-12-27

    We report here on the recent {beta}-decay studies made at ISOL-type Facilities to determine the multiparticle breakup mechanism of excited states in light nuclei by studying them in full kinematics. In particular the results obtained for the A = 9 isobars and the breakup of the 12.7 MeV state in {sup 12}C of unnatural parity are discussed. The breakup of the latter has been debated since more than a decade. Mirror beta transitions in the A=9 chain are compared and a large asymmetry factor is deduced for the transitions to high excitation energy in {sup 9}Be (11.8 MeV) and {sup 9}B (12.2 MeV) fed in the {beta}-decay of {sup 9}Li and {sup 9}C respectively. It is shown that the asymmetry is not due to experimental problems or differences in the mechanisms of breakup or in the spin of the states. As no asymmetry is found in the gs to gs transition it must be due to the particular structure of these excited states. The controversy on the breakup mechanism of the 12.7 MeV state is resolved.

  14. Extremotolerant survival and proteomics of Acinetobacter isolated from spacecraft assembly facilities

    Science.gov (United States)

    Mogul, Rakesh; Vaishampayan, Parag; Venkateswaran, Kasthuri; McCoy, Kelly; Derecho, Ivy; Dallal, Freida

    2012-07-01

    Herein, we report on the extreme hydrogen peroxide resistance of Acinetobacter isolated from the assembly facilities for the Mars Odyssey orbiter and Phoenix lander. Specific activity experiments on 10 different spacecraft-associated Acinetobacter strains show that the catalase contents are 15-250-fold greater than that of E. coli. Among this group, the highest and lowest catalase-containing strains, which were Acinetobacter nov. sp. 2P01AA and Acinetobacter radioresistens 50v1, demonstrated no significant and 2-log reductions in survivability upon exposure to 100 mM hydrogen peroxide (1 hr), respectively. These survivals are among the highest reported for non-spore forming Gram-negative bacteria. Comparative proteomics on these strains reveals that alkyl hydroperoxide reductase, ATP synthase, dihydrolipoamide dehydrogenase, and peptidyl-tRNA hydrolase also contribute to the hydrogen peroxide extremotolerance. Together, the survival and metabolic features of the spacecraft-associated Acinetobacter indicate that survival in the dry and low-nutrient environments of clean rooms is supported by factors such as oxidant degradation, energy management, and protein biosynthesis.

  15. Next generation gamma-ray Cherenkov detectors for the National Ignition Facility

    Science.gov (United States)

    Herrmann, H. W.; Kim, Y. H.; McEvoy, A. M.; Zylstra, A. B.; Young, C. S.; Lopez, F. E.; Griego, J. R.; Fatherley, V. E.; Oertel, J. A.; Stoeffl, W.; Khater, H.; Hernandez, J. E.; Carpenter, A.; Rubery, M. S.; Horsfield, C. J.; Gales, S.; Leatherland, A.; Hilsabeck, T.; Kilkenny, J. D.; Malone, R. M.; Hares, J. D.; Milnes, J.; Shmayda, W. T.; Stoeckl, C.; Batha, S. H.

    2016-11-01

    The newest generation of Gas Cherenkov Detector (GCD-3) employed in Inertial Confinement Fusion experiments at the Omega Laser Facility has provided improved performance over previous generations. Comparison of reaction histories measured using two different deuterium-tritium fusion products, namely gamma rays using GCD and neutrons using Neutron Temporal Diagnostic (NTD), have provided added credibility to both techniques. GCD-3 is now being brought to the National Ignition Facility (NIF) to supplement the existing Gamma Reaction History (GRH-6m) located 6 m from target chamber center (TCC). Initially it will be located in a reentrant well located 3.9 m from TCC. Data from GCD-3 will inform the design of a heavily-shielded "Super" GCD to be located as close as 20 cm from TCC. It will also provide a test-bed for faster optical detectors, potentially lowering the temporal resolution from the current ˜100 ps state-of-the-art photomultiplier tubes (PMT) to ˜10 ps Pulse Dilation PMT technology currently under development.

  16. The impact of regulatory compliance behavior on hazardous waste generation in European private healthcare facilities.

    Science.gov (United States)

    Botelho, Anabela

    2013-10-01

    This study empirically evaluates whether the increasingly large numbers of private outpatient healthcare facilities (HCFs) within the European Union (EU) countries comply with the existing European waste legislation, and whether compliance with such legislation affects the fraction of healthcare waste (HCW) classified as hazardous. To that end, this study uses data collected by a large survey of more than 700 small private HCFs distributed throughout Portugal, a full member of the EU since 1986, where 50% of outpatient care is currently dominated by private operators. The collected data are then used to estimate a hurdle model, i.e. a statistical specification in which there are two processes: one is the process by which some HCFs generate zero or some positive fraction of hazardous HCW, and another is the process by which HCFs generate a specific positive fraction of hazardous HCW conditional on producing any. Taken together, the results show that although compliance with the law is far from ideal, it is the strongest factor influencing hazardous waste generation. In particular, it is found that higher compliance has a small and insignificant effect on the probability of generating (or reporting) positive amounts of hazardous waste, but it does have a large and significant effect on the fraction of hazardous waste produced, conditional on producing any, with a unit increase in the compliance rate leading to an estimated decrease in the fraction of hazardous HCW by 16.3 percentage points.

  17. 78 FR 14842 - Crystal River Nuclear Generating Plant, Unit 3; Application for Renewal of License to Facility...

    Science.gov (United States)

    2013-03-07

    ... COMMISSION Crystal River Nuclear Generating Plant, Unit 3; Application for Renewal of License to Facility... operate the Crystal River Nuclear Generating Plant, Unit 3 (CR3), at 2609 megawatts thermal. The FPC... located near Crystal River, FL; the current operating license for the CR3 expires on December 3, 2016. The...

  18. Hazardous medical waste generation in Greece: case studies from medical facilities in Attica and from a small insular hospital.

    Science.gov (United States)

    Komilis, Dimitrios; Katsafaros, Nikolaos; Vassilopoulos, Panagiotis

    2011-08-01

    The accurate calculation of the unit generation rates and composition of medical waste generated from medical facilities is necessary in order to design medical waste treatment systems. In this work, the unit medical waste generation rates of 95 public and private medical facilities in the Attica region were calculated based on daily weight records from a central medical waste incineration facility. The calculated medical waste generation rates (in kg bed(-1) day( -1)) varied widely with average values at 0.27 ± 113% and 0.24 ± 121%, for public and private medical facilities, respectively. The hazardous medical waste generation was measured, at the source, in the 40 bed hospital of the island of Ikaria for a period of 42 days during a 6 month period. The average hazardous medical waste generation rate was 1.204 kg occupied bed(-1) day(-1) or 0.33 kg (official) bed( -1) day(-1). From the above amounts, 54% resulted from the patients' room (solid and liquid wastes combined), 24% from the emergency department (solid waste), 17% from the clinical pathology lab and 6% from the X-ray lab. In average, 17% of the total hazardous medical waste was solely infectious. Conclusively, no correlation among the number of beds and the unit medical waste generation rate could be established. Each hospital should be studied separately, since medical waste generation and composition depends on the number and type of departments/laboratories at each hospital, number of external patients and number of occupied beds.

  19. X-ray generation by inverse Compton scattering at the superconducting RF test facility

    Energy Technology Data Exchange (ETDEWEB)

    Shimizu, Hirotaka, E-mail: hirotaka@post.kek.jp [KEK, 1-1 Oho, Tsukuba 305-0801, Ibaraki (Japan); Akemoto, Mitsuo; Arai, Yasuo; Araki, Sakae; Aryshev, Alexander; Fukuda, Masafumi; Fukuda, Shigeki; Haba, Junji; Hara, Kazufumi; Hayano, Hitoshi; Higashi, Yasuo; Honda, Yosuke; Honma, Teruya; Kako, Eiji; Kojima, Yuji; Kondo, Yoshinari; Lekomtsev, Konstantin; Matsumoto, Toshihiro; Michizono, Shinichiro; Miyoshi, Toshinobu [KEK, 1-1 Oho, Tsukuba 305-0801, Ibaraki (Japan); and others

    2015-02-01

    Quasi-monochromatic X-rays with high brightness have a broad range of applications in fields such as life sciences, bio-, medical applications, and microlithography. One method for generating such X-rays is via inverse Compton scattering (ICS). X-ray generation experiments using ICS were carried out at the superconducting RF test facility (STF) accelerator at KEK. A new beam line, newly developed four-mirror optical cavity system, and new X-ray detector system were prepared for experiments downstream section of the STF electron accelerator. Amplified pulsed photons were accumulated into a four-mirror optical cavity and collided with an incoming 40 MeV electron beam. The generated X-rays were detected using a microchannel plate (MCP) detector for X-ray yield measurements and a new silicon-on-insulator (SOI) detector system for energy measurements. The detected X-ray yield by the MCP detector was 1756.8±272.2 photons/(244 electron bunches). To extrapolate this result to 1 ms train length under 5 Hz operations, 4.60×10{sup 5} photons/1%-bandwidth were obtained. The peak X-ray energy, which was confirmed by the SOI detector, was 29 keV, and this is consistent with ICS X-rays.

  20. USE OF PRODUCED WATER IN RECIRCULATING COOLING SYSTEMS AT POWER GENERATING FACILITIES

    Energy Technology Data Exchange (ETDEWEB)

    Michael N. DiFilippo

    2004-08-01

    The purpose of this study is to evaluate produced water as a supplemental source of water for the San Juan Generating Station (SJGS). This study incorporates elements that identify produced water volume and quality, infrastructure to deliver it to SJGS, treatment requirements to use it at the plant, delivery and treatment economics, etc. SJGS, which is operated by Public Service of New Mexico (PNM) is located about 15 miles northwest of Farmington, New Mexico. It has four units with a total generating capacity of about 1,800 MW. The plant uses 22,400 acre-feet of water per year from the San Juan River with most of its demand resulting from cooling tower make-up. The plant is a zero liquid discharge facility and, as such, is well practiced in efficient water use and reuse. For the past few years, New Mexico has been suffering from a severe drought. Climate researchers are predicting the return of very dry weather over the next 30 to 40 years. Concern over the drought has spurred interest in evaluating the use of otherwise unusable saline waters. Deliverable 2 focuses on transportation--the largest obstacle to produced water reuse in the San Juan Basin (the Basin). Most of the produced water in the Basin is stored in tanks at the well head and must be transported by truck to salt water disposal (SWD) facilities prior to injection. Produced water transportation requirements from the well head to SJGS and the availability of existing infrastructure to transport the water are discussed in this deliverable.

  1. Generation of bright isolated attosecond soft X-ray pulses driven by multicycle midinfrared lasers.

    Science.gov (United States)

    Chen, Ming-Chang; Mancuso, Christopher; Hernández-García, Carlos; Dollar, Franklin; Galloway, Ben; Popmintchev, Dimitar; Huang, Pei-Chi; Walker, Barry; Plaja, Luis; Jaroń-Becker, Agnieszka A; Becker, Andreas; Murnane, Margaret M; Kapteyn, Henry C; Popmintchev, Tenio

    2014-06-10

    High harmonic generation driven by femtosecond lasers makes it possible to capture the fastest dynamics in molecules and materials. However, to date the shortest subfemtosecond (attosecond, 10(-18) s) pulses have been produced only in the extreme UV region of the spectrum below 100 eV, which limits the range of materials and molecular systems that can be explored. Here we experimentally demonstrate a remarkable convergence of physics: when midinfrared lasers are used to drive high harmonic generation, the conditions for optimal bright, soft X-ray generation naturally coincide with the generation of isolated attosecond pulses. The temporal window over which phase matching occurs shrinks rapidly with increasing driving laser wavelength, to the extent that bright isolated attosecond pulses are the norm for 2-µm driving lasers. Harnessing this realization, we experimentally demonstrate the generation of isolated soft X-ray attosecond pulses at photon energies up to 180 eV for the first time, to our knowledge, with a transform limit of 35 attoseconds (as), and a predicted linear chirp of 300 as. Most surprisingly, advanced theory shows that in contrast with as pulse generation in the extreme UV, long-duration, 10-cycle, driving laser pulses are required to generate isolated soft X-ray bursts efficiently, to mitigate group velocity walk-off between the laser and the X-ray fields that otherwise limit the conversion efficiency. Our work demonstrates a clear and straightforward approach for robustly generating bright isolated attosecond pulses of electromagnetic radiation throughout the soft X-ray region of the spectrum.

  2. [Generation of superoxide radicals by the mitochondrial respiratory chain of isolated cardiomyocytes].

    Science.gov (United States)

    Kashkarov, K P; Vasil'eva, E V; Ruuge, E K

    1994-06-01

    Generation of superoxide radicals by the mitochondrial respiratory chain of cardiomyocites isolated from rat heart and treated with saponin was studied. The rate of O2- production was measured by electron paramagnetic resonance (EPR) spectroscopy using hydroxylamine TEMPONE-H as spin trap. A device has been constructed which provided permanent stirring of cardiomyocyte samples directly in the cavity and prevented cell aggregation. When substrates and antimycin A and/or rotenone are added, the radical production rate increased and reached its maximum in the presence of the both inhibitors. Superoxide dismutase as well as KCN suppressed the radical production, thus being suggestive of the generation of superoxide radicals in the bc1 complex, while the mechanism of O2- production is the same as was suggested for isolated mitochondria. The ratio between rates of O2- generation by isolated cardiomyocytes under various experimental conditions is in a good accord with corresponding parameter of isolated mitochondria. However, in the case of cardiomyocytes the absolute values of the O2- production rate are approximately twice as high as those in isolated mitochondria, presumably due to the partial damage of the mitochondrial respiratory chain during the isolation procedure.

  3. Position paper on gas generation in the Waste Isolation Pilot Plant

    Energy Technology Data Exchange (ETDEWEB)

    Brush, L.H.

    1994-11-15

    Gas generation by transuranic (TRU) waste is a significant issue because gas will, if produced in significant quantities, affect the performance of the Waste Isolation Pilot Plant (WIPP) with respect to Environmental Protection Agency (EPA) regulations for the long-term isolation of radioactive and chemically hazardous waste. If significant gas production occurs, it will also affect, and will be affected by, other processes and parameters in WIPP disposal rooms. The processes that will produce gas in WIPP disposal rooms are corrosion, microbial activity and radiolysis. This position paper describes these processes and the models, assumptions and data used to predict gas generation in WIPP disposal rooms.

  4. Sensor fault detection and isolation in doubly-fed induction generators accounting for parameter variations

    Energy Technology Data Exchange (ETDEWEB)

    Galvez-Carrillo, Manuel; Kinnaert, Michel [Dept. of Control Engineering and System Analysis, Universite Libre de Bruxelles (ULB), 50 Av. F.D. Roosevelt, CP 165/55, B-1050 Brussels (Belgium)

    2011-05-15

    A fault detection and isolation (FDI) system for monitoring rotor current sensors in a doubly-fed induction generator (DFIG) for wind turbine applications is presented. The FDI system is designed so that the effect of parameter variations (resistances and inductances) is minimized. The residual generation is based on the generalized observer scheme (GOS) including parameter estimation. A decision system made of a combination of vector CUSUM (Cumulative sum) algorithms is used to process the residual vector and to achieve detection and isolation of incipient (small magnitude) faults. The approach is validated using signals obtained from a simulated vector-controlled DFIG. (author)

  5. A Study on Applicability of Distributed Energy Generation, Storage and Consumption within Small Scale Facilities

    Directory of Open Access Journals (Sweden)

    Jesús Rodríguez-Molina

    2016-09-01

    Full Text Available Distributed generation and storage of energy, conceived as one of the prominent applications of the Smart Grid, has become one of the most popular ways for generation and usage of electricity. Not only does it offer environmental advantages and a more decentralized way to produce energy, but it also enables former consumers to become producers (thus turning them into prosumers. Alternatively, regular power production and consumption is still widely used in most of the world. Unfortunately, accurate business models representations and descriptive use cases for small scale facilitates, either involved in distributed energy or not, have not been provided in a descriptive enough manner. What is more, the possibilities that electricity trade and its storage and consumption activities offer for small users to obtain profits are yet to be addressed and offered to the research community in a thorough manner, so that small consumers will use them to their advantage. This paper puts forward a study on four different business models for small scale facilities and offers an economical study on how they can be deployed as a way to offer profitability for end users and new companies, while at the same time showing the required technological background to have them implemented.

  6. Fair division of generation profile and fuel consumption in isolated micro-grids

    DEFF Research Database (Denmark)

    Kosek, Anna Magdalena; Heussen, Kai

    2014-01-01

    leads to more stressed loading conditions of diesel generators. We propose a control strategy employing fair division of generator allocation using a compensation procedure based on social choice methods. A co-simulation set up with separate power system and control strategy simulators is used......Islands and rural areas can decrease their cost of energy by exploiting renewable energy as compared to diesel- only generation. Operation of such isolated micro-grids requires allocation of units for grid stability. Depending on the control strategy employed, the fluctuating renewable production...... to provide a proof-of-concept case study of an isolated micro-grid with two wind turbines and three diesel generators. In comparison with a simple master- slave allocation, the proposed coordination scheme improves the distribution of fuel allocation by 27,5%, reduces under-load time by 43,5% and decreases...

  7. Interim reclamation report, Basalt Waste Isolation Project Near Surface Test Facility 1990

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, C.A.; Rickard, W.H. Jr.; Hefty, M.G.; Cadoret, N.A.

    1991-01-01

    This report describes the development of the reclamation project for the Hanford Site Near Surface Test Facility (NSTF), its implementation, and preliminary estimates of its success. The goal of the reclamation project is to return disturbed sites as nearly as practicable to their original conditions using native species. Gable Mountain is dominated by two plant communities: a big sagebrush (Artemisia tridentata) -- Sandberg's bluegrass (Poa sandbergii) community and a stiff sagebrush (Artemisia rigida) -- Sandberg's bluegrass community. Disassembly of the site installations began on March 15, 1988, and the site was returned to original contours by December 12, 1988. Two separate revegetation methods were employed at the NSTF to meet differing site constraints. Vegetative cover and density in the revegetation plots were assessed in April 1989 and again in June 1989 and 1990. It is extremely unlikely that the sand pit, borrow pit, box cuts, generator pad area, or ventilation fan area will reach the reclamation objectives set for these areas within the next 50 years without further intervention. These areas currently support few living plants. Vegetation on revegetated native soils appears to be growing as expected. Vegetation growth on the main waterline is well below the objective. To date, no shrubs have grown on the area, growth of native grasses is well below the objective, and much of the area has been covered with the pit run material, which may not support adequate growth. Without further treatments, the areas without the pit run material will likely revert to a nearly pure cheatgrass condition. 44 refs., 13 figs., 7 tabs.

  8. Perspectives on Gamma-Ray Burst Physics and Cosmology with Next Generation Facilities

    Science.gov (United States)

    Yuan, Weimin; Amati, Lorenzo; Cannizzo, John K.; Cordier, Bertrand; Gehrels, Neil; Ghirlanda, Giancarlo; Götz, Diego; Produit, Nicolas; Qiu, Yulei; Sun, Jianchao; Tanvir, Nial R.; Wei, Jianyan; Zhang, Chen

    2016-12-01

    High-redshift Gamma-Ray Bursts (GRBs) beyond redshift {˜}6 are potentially powerful tools to probe the distant early Universe. Their detections in large numbers and at truly high redshifts call for the next generation of high-energy wide-field instruments with unprecedented sensitivity at least one order of magnitude higher than the ones currently in orbit. On the other hand, follow-up observations of the afterglows of high-redshift GRBs and identification of their host galaxies, which would be difficult for the currently operating telescopes, require new, extremely large facilities of at multi-wavelengths. This chapter describes future experiments that are expected to advance this exciting field, both being currently built and being proposed. The legacy of Swift will be continued by SVOM, which is equipped with a set of space-based multi-wavelength instruments as well as and a ground segment including a wide angle camera and two follow-up telescopes. The established Lobster-eye X-ray focusing optics provides a promising technology for the detection of faint GRBs at very large distances, based on which the THESEUS, Einstein Probe and other mission concepts have been proposed. Follow-up observations and exploration of the reionization era will be enabled by large facilities such as SKA in the radio, the 30 m class telescopes in the optical/near-IR, and the space-borne WFIRST and JWST in the optical/near-IR/mid-IR. In addition, the X-ray and γ-ray polarization experiment POLAR is also introduced.

  9. Perspectives on Gamma-Ray Burst Physics and Cosmology with Next Generation Facilities

    Science.gov (United States)

    Yuan, Weimin; Amati, Lorenzo; Cannizzo, John K.; Cordier, Bertrand; Gehrels, Neil; Ghirlanda, Giancarlo; Götz, Diego; Produit, Nicolas; Qiu, Yulei; Sun, Jianchao; Tanvir, Nial R.; Wei, Jianyan; Zhang, Chen

    2016-07-01

    High-redshift Gamma-Ray Bursts (GRBs) beyond redshift {˜}6 are potentially powerful tools to probe the distant early Universe. Their detections in large numbers and at truly high redshifts call for the next generation of high-energy wide-field instruments with unprecedented sensitivity at least one order of magnitude higher than the ones currently in orbit. On the other hand, follow-up observations of the afterglows of high-redshift GRBs and identification of their host galaxies, which would be difficult for the currently operating telescopes, require new, extremely large facilities of at multi-wavelengths. This chapter describes future experiments that are expected to advance this exciting field, both being currently built and being proposed. The legacy of Swift will be continued by SVOM, which is equipped with a set of space-based multi-wavelength instruments as well as and a ground segment including a wide angle camera and two follow-up telescopes. The established Lobster-eye X-ray focusing optics provides a promising technology for the detection of faint GRBs at very large distances, based on which the THESEUS, Einstein Probe and other mission concepts have been proposed. Follow-up observations and exploration of the reionization era will be enabled by large facilities such as SKA in the radio, the 30 m class telescopes in the optical/near-IR, and the space-borne WFIRST and JWST in the optical/near-IR/mid-IR. In addition, the X-ray and γ-ray polarization experiment POLAR is also introduced.

  10. Experimental infection of one-day-old chicks with Salmonella Serotypes Previously isolated from poultry facilities, wild birds, and swine

    Directory of Open Access Journals (Sweden)

    E de Sousa

    2013-12-01

    Full Text Available In order to maintain the high production and export rates achieved by the Brazilian poultry industry, it is necessary to prevent and control certain disease agents, such as Salmonella spp. Using bacterial cultures, the aim of the present study was to investigate the prevalence of Salmonella spp. in specimens collected from broiler facilities. Local wild birds were also sampled, as well as the feces of swine housed on the poultry farm. After sample collection, the isolated serotypes were subsequently inoculated into broiler chicks to determine their effects. Positive samples were collected from the following locations in the poultry facilities: poultry litter (S. serotype 4,5,12:R:-; S. Heidelberg; S. Infantis, broiler feces (S. Heidelberg; S. serotype 6,7:R:-; S. serotype 4,5,12:R:-; S. Tennessee, water (S. Glostrup; S. serotype 6,8:d:-;, and lesser mealworms (Alphitobius diaperinus found in the litter (S. Tennessee. Among the 36 wild birds captured, S. Heidelberg was isolated from one bird's organs and intestinal contents (Colaptes campestris, and S. Enteritidis was isolated from another bird's intestinal contents (Zenaida auriculata. Salmonella Panama and Salmonella Typhimurium were isolated from swine feces. One-day-old chicks (150 were divided into 10 groups of 15 animals each. Each group was orally inoculated with a previously isolated serotype of Salmonella. Soft stools were observed on the cage floor and around the birds' cloaca between 3 and 12 days post-infection (dpi. The different serotypes of Salmonella used to inoculate the chicks were re-isolated from the spleen, liver, and cecal content samples of the infected birds on 15 and 21 dpi.

  11. Thirty-year solid waste generation forecast for facilities at SRS

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    The information supplied by this 30-year solid waste forecast has been compiled as a source document to the Waste Management Environmental Impact Statement (WMEIS). The WMEIS will help to select a sitewide strategic approach to managing present and future Savannah River Site (SRS) waste generated from ongoing operations, environmental restoration (ER) activities, transition from nuclear production to other missions, and decontamination and decommissioning (D&D) programs. The EIS will support project-level decisions on the operation of specific treatment, storage, and disposal facilities within the near term (10 years or less). In addition, the EIS will provide a baseline for analysis of future waste management activities and a basis for the evaluation of the specific waste management alternatives. This 30-year solid waste forecast will be used as the initial basis for the EIS decision-making process. The Site generates and manages many types and categories of waste. With a few exceptions, waste types are divided into two broad groups-high-level waste and solid waste. High-level waste consists primarily of liquid radioactive waste, which is addressed in a separate forecast and is not discussed further in this document. The waste types discussed in this solid waste forecast are sanitary waste, hazardous waste, low-level mixed waste, low-level radioactive waste, and transuranic waste. As activities at SRS change from primarily production to primarily decontamination and decommissioning and environmental restoration, the volume of each waste s being managed will change significantly. This report acknowledges the changes in Site Missions when developing the 30-year solid waste forecast.

  12. Perspectives on Gamma-Ray Burst Physics and Cosmology with Next Generation Facilities

    CERN Document Server

    Yuan, Weimin; Cannizzo, John K; Cordier, Bertrand; Gehrels, Neil; Ghirlanda, Giancarlo; Götz, Diego; Produit, Nicolas; Qiu, Yulei; Sun, Jianchao; Tanvir, Nial R; Wei, Jianyan; Zhang, Chen

    2016-01-01

    High-redshift Gamma-Ray Bursts (GRBs) beyond redshift $\\sim6$ are potentially powerful tools to probe the distant early Universe. Their detections in large numbers and at truly high redshifts call for the next generation of high-energy wide-field instruments with unprecedented sensitivity at least one order of magnitude higher than the ones currently in orbit. On the other hand, follow-up observations of the afterglows of high-redshift GRBs and identification of their host galaxies, which would be difficult for the currently operating telescopes, require new, extremely large facilities of at multi-wavelengths. This chapter describes future experiments that are expected to advance this exciting field, both being currently built and being proposed. The legacy of Swift will be continued by SVOM, which is equipped with a set of space-based multi-wavelength instruments as well as and a ground segment including a wide angle camera and two follow-up telescopes. The established Lobster-eye X-ray focusing optics provi...

  13. The ZECOMIX experimental facility for hydrogen and power generation from coal

    Energy Technology Data Exchange (ETDEWEB)

    A. Calabro; P. Deiana; P. Fiorini; S. Stendardo; G. Girardi [ENEA - Italian Agency for New Technologies, Rome (Italy). Energy and Environment Energy and Plants Division

    2006-07-01

    The Zecomix project, conceived by ENEA in the framework of Italian National Hydrogen Project, is aimed at studying an integrated process that produces both hydrogen and electricity from coal, with zero emissions and very high efficiency. The Zero Emission Coal Mixed technology concept combines two different systems: the Zero Emission Coal gasification and the Zero Emission Combustion Technology based on Hydrogen-fuelled internal combustion turbine cycle. The key element is the integration of a gasification process, characterized by coal hydrogasification technology and carbon dioxide sequestration, with the power island, where an oxy-combustion occurs. The experimental facility will be realized at the ENEA Research Centre of Casaccia at about thirty kilometres from the centre of Rome. It consists of a very flexible plant, in which more components can be tested separately or connected together. The plant is provided with an atmospheric fixed bed gasifier coal and a carbonator/calcinator reactor; moreover a pressurized hydrogasifier reactor and a 100 kWe microturbine test bench are present. Other auxiliary components are a gas mixing system, for hydrogen-based syngas production, and a 200 kW steam generator. 5 refs., 5 figs., 1 tab.

  14. Project overview and update on WEAVE: the next generation wide-field spectroscopy facility for the William Herschel Telescope

    NARCIS (Netherlands)

    Dalton, Gavin; Trager, Scott; Abrams, Don Carlos; Bonifacio, Piercarlo; López Aguerri, J. Alfonso; Middleton, Kevin; Benn, Chris; Dee, Kevin; Sayède, Frédéric; Lewis, Ian; Pragt, Johan; Pico, Sergio; Walton, Nic; Rey, Juerg; Allende Prieto, Carlos; Peñate, José; Lhome, Emilie; Agócs, Tibor; Alonso, José; Terrett, David; Brock, Matthew; Gilbert, James; Ridings, Andy; Guinouard, Isabelle; Verheijen, Mark; Tosh, Ian; Rogers, Kevin; Steele, Iain; Stuik, Remko; Tromp, Neils; Jasko, Attila; Kragt, Jan; Lesman, Dirk; Mottram, Chris; Bates, Stuart; Gribbin, Frank; Rodriguez, Luis Fernando; Delgado, José M.; Martin, Carlos; Cano, Diego; Navarro, Ramón; Irwin, Mike; Lewis, Jim; Gonzalez Solares, Eduardo; O'Mahony, Neil; Bianco, Andrea; Zurita, Christina; ter Horst, Rik; Molinari, Emilio; Lodi, Marcello; Guerra, José; Vallenari, Antonella; Baruffolo, Andrea

    2014-01-01

    We present an overview of and status report on the WEAVE next-generation spectroscopy facility for the William Herschel Telescope (WHT). WEAVE principally targets optical ground-based follow up of upcoming ground-based (LOFAR) and space-based (Gaia) surveys. WEAVE is a multi-object and multi-IFU fac

  15. A stepwise procedure for isolation of murine bone marrow and generation of dendritic cells

    Directory of Open Access Journals (Sweden)

    Alka Madaan

    2014-02-01

    Full Text Available Bone marrow derived Dendritic cells (BMDCs are routinely employed in cell based assays to evaluate immunomodulatory and anti-inflammatory activities. Hence, simplified, stepwise, defined and standardized methods are required for isolation of bone marrow cells from mice, propagating them in presence of growth factors and obtaining high and reproducible yields of BMDCs. Here, we describe a detailed, stepwise protocol with pictorial representation to isolate bone marrow from mouse femur and development of dendritic cells. Mouse bone marrow cells are cultured in presence of granulocyte-macrophage colony stimulating factor (GM-CSF for 6 days to generate BMDCs.

  16. Enterobacteriaceae and related organisms isolated from nest run cart shelves in commercial shell egg processing facilities

    Science.gov (United States)

    Enterobacteriaceae, including Salmonella may be recovered from foods and processing facilities. High levels of Enterobacteriaceae in the processing plant environment can be an indication of inadequate sanitation. This experiment was designed to determine if nest run egg carts serve as reservoirs ...

  17. Tests of the space gamma spectrometer prototype at the JINR experimental facility with different types of neutron generators

    Science.gov (United States)

    Litvak, M. L.; Vostrukhin, A. A.; Golovin, D. V.; Dubasov, P. V.; Zontikov, A. O.; Kozyrev, A. S.; Krylov, A. R.; Krylov, V. A.; Mitrofanov, I. G.; Mokrousov, M. I.; Repkin, A. N.; Timoshenko, G. N.; Udovichenko, K. V.; Shvetsov, V. N.

    2017-07-01

    The results of the tests of the HPGe gamma spectrometer performed with a planetary soil model and different types of pulse neutron generators are presented. All measurements have been performed at the experimental nuclear planetary science facility (Joint Institute for Nuclear Research) for the physical calibration of active gamma and neutron spectrometers. The aim of the study is to model a space experiment on determining the elemental composition of Martian planetary matter by neutron-induced gamma spectroscopy. The advantages and disadvantages of a gas-filled neutron generator in comparison with a vacuum-tube neutron generator are examined.

  18. Effect of steam generator configuration in a loss of the RHR during mid-loop operation at PKL facility

    Energy Technology Data Exchange (ETDEWEB)

    Villanueva, J. F.; Carlos, S.; Martorell, S.; Sanchez, F. [Dpto. Ingenieria Quimica Y Nuclear, Universitat Politecnica de Valencia, Camino Vera s/n, 46022 Valencia (Spain)

    2012-07-01

    The loss of the residual heat removal system in mid-loop conditions may occur with a non-negligible contribution to the plant risk, so the analysis of the accidental sequences and the actions to mitigate the accident are of great interest in shutdown conditions. In order to plan the appropriate measures to mitigate the accident is necessary to understand the thermal-hydraulic processes following the loss of the residual heat removal system during shutdown. Thus, transients of this kind have been simulated using best-estimate codes in different integral test facilities and compared with experimental data obtained in different facilities. In PKL (Primaerkreislauf-Versuchsanlage, primary coolant loop test facility) test facility different series of experiments have been undertaken to analyze the plant response in shutdown. In this context, the E3 and F2 series consist of analyzing the loss of the residual heat removal system with a reduced inventory in the primary system. In particular, the experiments were developed to investigate the influence of the steam generators secondary side configuration on the plant response, what involves the consideration of different number of steam generators filled with water and ready for activation, on the heat transfer mechanisms inside the steam generators U-tubes. This work presents the results of such experiments calculated using, RELAP5/Mod 3.3. (authors)

  19. Improved Detection by Next-Generation Sequencing of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates

    OpenAIRE

    Maningi, Nontuthuko E.; Daum, Luke T.; Rodriguez, John D.; Mphahlele, Matsie; Peters, Remco P. H.; Fischer, Gerald W.; Chambers, James P.; Fourie, P. Bernard

    2015-01-01

    The technical limitations of common tests used for detecting pyrazinamide (PZA) resistance in Mycobacterium tuberculosis isolates pose challenges for comprehensive and accurate descriptions of drug resistance in patients with multidrug-resistant tuberculosis (MDR-TB). In this study, a 606-bp fragment (comprising the pncA coding region plus the promoter) was sequenced using Ion Torrent next-generation sequencing (NGS) to detect associated PZA resistance mutations in 88 recultured MDR-TB isolat...

  20. Improved Detection by Next-Generation Sequencing of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates.

    Science.gov (United States)

    Maningi, Nontuthuko E; Daum, Luke T; Rodriguez, John D; Mphahlele, Matsie; Peters, Remco P H; Fischer, Gerald W; Chambers, James P; Fourie, P Bernard

    2015-12-01

    The technical limitations of common tests used for detecting pyrazinamide (PZA) resistance in Mycobacterium tuberculosis isolates pose challenges for comprehensive and accurate descriptions of drug resistance in patients with multidrug-resistant tuberculosis (MDR-TB). In this study, a 606-bp fragment (comprising the pncA coding region plus the promoter) was sequenced using Ion Torrent next-generation sequencing (NGS) to detect associated PZA resistance mutations in 88 recultured MDR-TB isolates from an archived series collected in 2001. These 88 isolates were previously Sanger sequenced, with 55 (61%) designated as carrying the wild-type pncA gene and 33 (37%) showing mutations. PZA susceptibility of the isolates was also determined using the Bactec 460 TB system and the Wayne test. In this study, isolates were recultured and susceptibility testing was performed in Bactec 960 MGIT. Concordance between NGS and MGIT results was 93% (n = 88), and concordance values between the Bactec 460, the Wayne test, or pncA gene Sanger sequencing and NGS results were 82% (n = 88), 83% (n = 88), and 89% (n = 88), respectively. NGS confirmed the majority of pncA mutations detected by Sanger sequencing but revealed several new and mixed-strain mutations that resolved discordancy in other phenotypic results. Importantly, in 53% (18/34) of these isolates, pncA mutations were located in the 151 to 360 region and warrant further exploration. In these isolates, with their known resistance to rifampin, NGS of pncA improved PZA resistance detection sensitivity to 97% and specificity to 94% using NGS as the gold standard and helped to resolve discordant results from conventional methodologies.

  1. On prospects of powerful SXR pulse generation on “Gamma 4” facility

    Science.gov (United States)

    Gordeev, V. S.; Grishin, A. V.; Orlov, A. P.; Mozgovoy, A. L.; Repin, B. G.; Glushkov, S. L.; Puchagin, S. Yu.; Strabykin, K. V.

    2014-08-01

    RFNC-VNIIEF is creating a multi-module multi-terawatt electro-physical facility “Gamma”, intended for research in the field of radiation physics. Up to now RFNC-VNIIEF created the first module of the facility - a high-current pulsed electron accelerator “Gamma-1”, having the following parameters: boundary energy of the electrons of ≤ 2.0 MeV, diode current of 0.75 MA, maximum electric power of 1.5 TW. Creation of the 4-module variant of the facility (Gamma-4) is the next stage of the work. This paper presents calculation research of expected characteristics of soft x-ray radiation output pulse at “Gamma-4” facility operation into a common load (Z-pinch).

  2. Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms

    Science.gov (United States)

    Zhang, Qiang; Wang, Tingting; Zhou, Qian; Zhang, Peng; Gong, Yanhai; Gou, Honglei; Xu, Jian; Ma, Bo

    2017-01-01

    Wider application of single-cell analysis has been limited by the lack of an easy-to-use and low-cost strategy for single-cell isolation that can be directly coupled to single-cell sequencing and single-cell cultivation, especially for small-size microbes. Herein, a facile droplet microfluidic platform was developed to dispense individual microbial cells into conventional standard containers for downstream analysis. Functional parts for cell encapsulation, droplet inspection and sorting, as well as a chip-to-tube capillary interface were integrated on one single chip with simple architecture, and control of the droplet sorting was achieved by a low-cost solenoid microvalve. Using microalgal and yeast cells as models, single-cell isolation success rate of over 90% and single-cell cultivation success rate of 80% were demonstrated. We further showed that the individual cells isolated can be used in high-quality DNA and RNA analyses at both gene-specific and whole-genome levels (i.e. real-time quantitative PCR and genome sequencing). The simplicity and reliability of the method should improve accessibility of single-cell analysis and facilitate its wider application in microbiology researches. PMID:28112223

  3. ENIDINE: Vibration and seismic isolation technologies for power generation station applications

    Energy Technology Data Exchange (ETDEWEB)

    Zemanek, T.A.

    1994-12-31

    ENIDINE Inc. is a world leader in the design and manufacture of shock and vibration mounts. Founded in 1966, the company has two manufacturing facilities, employs over 300 people and supports a worldwide network of distributors and representatives. ENIDINE Inc. is part of the ENIDINE Corporate Group which owns a number of companies that design and manufacture Hydraulic/Pneumatic cylinders, Electromechanical devices, Hydraulic Control Valves and a number of Industrial Distribution companies throughout Europe. In total, the ENIDINE Corporate Group has over 900 employees with annual sales of over $100 million. ENIDINE shock and vibration mounts are used to isolate the vibration of missiles from their guidance systems, pumps from hospital operating equipment and off shore oil rigs, from the shock energy of waves in the North Sea. ENIDINE products can be found on all Boeing and McDonnell Douglas aircraft, as well as many electronic and weapons systems on board Navy ships.

  4. Optimizing charge breeding techniques for ISOL facilities in Europe: Conclusions from the EMILIE project

    Energy Technology Data Exchange (ETDEWEB)

    Delahaye, P., E-mail: delahaye@ganil.fr; Jardin, P.; Maunoury, L. [GANIL, CEA/DSM-CNRS/IN2P3, Blvd. Becquerel, BP 55027, 14076 Caen Cedex 05 (France); Galatà, A.; Patti, G. [INFN–Laboratori Nazionali di Legnaro, Viale dell’Università 2, 35020 Legnaro (Padova) (Italy); Angot, J.; Lamy, T.; Thuillier, T. [LPSC–Université Grenoble Alpes–CNRS/IN2P3, 53 rue des Martyrs, 38026 Grenoble Cedex (France); Cam, J. F.; Traykov, E.; Ban, G. [LPC Caen, 6 Blvd. Maréchal Juin, 14050 Caen Cedex (France); Celona, L. [INFN–Laboratori Nazionali del Sud, via S. Sofia 62, 95125 Catania (Italy); Choinski, J.; Gmaj, P. [Heavy Ion Laboratory, University of Warsaw, ul. Pasteura 5a, 02 093 Warsaw (Poland); Koivisto, H.; Kolhinen, V.; Tarvainen, O. [Department of Physics, University of Jyväskylä, PB 35 (YFL), 40351 Jyväskylä (Finland); Vondrasek, R. [Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, Illinois 60439 (United States); Wenander, F. [ISOLDE, CERN, 1211 Geneva 23 (Switzerland)

    2016-02-15

    The present paper summarizes the results obtained from the past few years in the framework of the Enhanced Multi-Ionization of short-Lived Isotopes for Eurisol (EMILIE) project. The EMILIE project aims at improving the charge breeding techniques with both Electron Cyclotron Resonance Ion Sources (ECRIS) and Electron Beam Ion Sources (EBISs) for European Radioactive Ion Beam (RIB) facilities. Within EMILIE, an original technique for debunching the beam from EBIS charge breeders is being developed, for making an optimal use of the capabilities of CW post-accelerators of the future facilities. Such a debunching technique should eventually resolve duty cycle and time structure issues which presently complicate the data-acquisition of experiments. The results of the first tests of this technique are reported here. In comparison with charge breeding with an EBIS, the ECRIS technique had lower performance in efficiency and attainable charge state for metallic ion beams and also suffered from issues related to beam contamination. In recent years, improvements have been made which significantly reduce the differences between the two techniques, making ECRIS charge breeding more attractive especially for CW machines producing intense beams. Upgraded versions of the Phoenix charge breeder, originally developed by LPSC, will be used at SPES and GANIL/SPIRAL. These two charge breeders have benefited from studies undertaken within EMILIE, which are also briefly summarized here.

  5. Facility Microgrids

    Energy Technology Data Exchange (ETDEWEB)

    Ye, Z.; Walling, R.; Miller, N.; Du, P.; Nelson, K.

    2005-05-01

    Microgrids are receiving a considerable interest from the power industry, partly because their business and technical structure shows promise as a means of taking full advantage of distributed generation. This report investigates three issues associated with facility microgrids: (1) Multiple-distributed generation facility microgrids' unintentional islanding protection, (2) Facility microgrids' response to bulk grid disturbances, and (3) Facility microgrids' intentional islanding.

  6. Modeling of high power pulse generator based on the non-linear elements of pulsed facilities

    Science.gov (United States)

    Averyanov, G. P.; Dmitrieva, V. V.; Kobylyatskiy, A. V.

    2017-01-01

    The article considered the software implementation mathematical model of the voltage pulse generator with a hard switch. The interactive object-oriented software interface provides the choice of generator parameters and the type of its load, as well as pulses parameters analysis on the load at the generator switching.

  7. A Voltage Regulator for Parallel Operated Isolated Synchronous Generators Using Statcom

    Directory of Open Access Journals (Sweden)

    Sravanthi Gudipati,

    2014-02-01

    Full Text Available Reactive power (vars is required to maintain the voltage to deliver active power through transmission lines. Generator and other loads require reactive power to convert the flow of electrons into useful work. When there is not enough reactive power, the voltage sags down and it is not possible to push the power demanded by loads through the lines. Thus reactive power is injected using the compensating device STATCOM.The Paper deals on static synchronous compensator (STATCOM which is connected to the parallel operated isolated synchronous generators to drive a 3-phase load. These generators are driven by constant prime mover like diesel engine,bio-mass,gasoline,steam turbine etc.Three single phase IGBT based Voltage source converter (VSC along with 3-single phase transformers and self supported D.C bus is used as a voltage controller. The project deals with controlling a STATCOM using non-linear techniques that involves hysteresis current control and Park’s transformation. The voltage controller of parallel operated generators for feeding 3-phase loads driven by constant speed prime mover like Hydraulic turbine governor model helps in satisfactory operation under varying loads and in load balancing. If the line current is less than the expected, the compensating current generated by STATCOM is taken by loads.

  8. Isolated sub-fs XUV pulse generation in Mn plasma ablation.

    Science.gov (United States)

    Ganeev, R A; Witting, T; Hutchison, C; Frank, F; Tudorovskaya, M; Lein, M; Okell, W A; Zaïr, A; Marangos, J P; Tisch, J W G

    2012-11-05

    We report studies of high-order harmonic generation in laser-produced manganese plasmas using sub-4-fs drive laser pulses. The measured spectra exhibit resonant enhancement of a small spectral region of about 2.5 eV width around the 31st harmonic (~50eV). The intensity contrast relative to the directly adjacent harmonics exceeds one order of magnitude. This finding is in sharp contrast to the results reported previously for multi-cycle laser pulses [Physical Review A 76, 023831 (2007)]. Theoretical modelling suggests that the enhanced harmonic emission forms an isolated sub-femtosecond pulse.

  9. Microbial Gas Generation Under Expected Waste Isolation Pilot Plant Repository Conditions: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gillow, J.B.; Francis, A.

    2011-07-01

    Gas generation from the microbial degradation of the organic constituents of transuranic (TRU) waste under conditions expected in the Waste Isolation Pilot Plant (WIPP) was investigated. The biodegradation of mixed cellulosic materials and electron-beam irradiated plastic and rubber materials (polyethylene, polyvinylchloride, hypalon, leaded hypalon, and neoprene) was examined. We evaluated the effects of environmental variables such as initial atmosphere (air or nitrogen), water content (humid ({approx}70% relative humidity, RH) and brine inundated), and nutrient amendments (nitogen phosphate, yeast extract, and excess nitrate) on microbial gas generation. Total gas production was determined by pressure measurement and carbon dioxide (CO{sub 2}) and methane (CH{sub 4}) were analyzed by gas chromatography; cellulose degradation products in solution were analyzed by high-performance liquid chromatography. Microbial populations in the samples were determined by direct microscopy and molecular analysis. The results of this work are summarized.

  10. Generation of isolated attosecond extreme ultraviolet pulses employing nanoplasmonic field enhancement: optimization of coupled ellipsoids

    Energy Technology Data Exchange (ETDEWEB)

    Stebbings, S L; Suessmann, F; Yang, Y-Y; Kling, M F [Max-Planck-Institut fuer Quantenoptik, Hans-Kopfermann-Strass e 1, 85748 Garching (Germany); Scrinzi, A [Ludwig-Maximilians-Universitaet Muenchen, Theresienstrasse 37, 80333 Muenchen (Germany); Durach, M; Rusina, A; Stockman, M I, E-mail: sarah.stebbings@mpq.mpg.de, E-mail: mstockman@gsu.edu, E-mail: matthias.kling@mpq.mpg.de [Department of Physics and Astronomy, Georgia State University, 29 Peachtree Center Avenue, Atlanta, GA 30303 (United States)

    2011-07-15

    The production of extreme ultraviolet (XUV) radiation via nanoplasmonic field-enhanced high-harmonic generation (HHG) in gold nanostructures at MHz repetition rates is investigated theoretically in this paper. Analytical and numerical calculations are employed and compared in order to determine the plasmonic fields in gold ellipsoidal nanoparticles. The comparison indicates that numerical calculations can accurately predict the field enhancement and plasmonic decay, but may encounter difficulties when attempting to predict the oscillatory behavior of the plasmonic field. Numerical calculations for coupled symmetric and asymmetric ellipsoids for different carrier-envelope phases (CEPs) of the driving laser field are combined with time-dependent Schroedinger equation simulations to predict the resulting HHG spectra. The studies reveal that the plasmonic field oscillations, which are controlled by the CEP of the driving laser field, play a more important role than the nanostructure configuration in finding the optimal conditions for the generation of isolated attosecond XUV pulses via nanoplasmonic field enhancement.

  11. Generation of isolated attosecond extreme ultraviolet pulses employing nanoplasmonic field enhancement: optimization of coupled ellipsoids

    Science.gov (United States)

    Stebbings, S. L.; Süßmann, F.; Yang, Y.-Y.; Scrinzi, A.; Durach, M.; Rusina, A.; Stockman, M. I.; Kling, M. F.

    2011-07-01

    The production of extreme ultraviolet (XUV) radiation via nanoplasmonic field-enhanced high-harmonic generation (HHG) in gold nanostructures at MHz repetition rates is investigated theoretically in this paper. Analytical and numerical calculations are employed and compared in order to determine the plasmonic fields in gold ellipsoidal nanoparticles. The comparison indicates that numerical calculations can accurately predict the field enhancement and plasmonic decay, but may encounter difficulties when attempting to predict the oscillatory behavior of the plasmonic field. Numerical calculations for coupled symmetric and asymmetric ellipsoids for different carrier-envelope phases (CEPs) of the driving laser field are combined with time-dependent Schrödinger equation simulations to predict the resulting HHG spectra. The studies reveal that the plasmonic field oscillations, which are controlled by the CEP of the driving laser field, play a more important role than the nanostructure configuration in finding the optimal conditions for the generation of isolated attosecond XUV pulses via nanoplasmonic field enhancement.

  12. Coordination Between Wind Power, Hydro Storage Facility and Conventional Generating Units According to the Annual Growth Load

    Directory of Open Access Journals (Sweden)

    Shahrokh Shojaeean

    2013-04-01

    Full Text Available Considering the growing trend of the consumption of the electric power and the global tendency to substitute new renewable sources of energy, this paper proposes a Monte Carlo based method to determine an optimal level of this change. Considering the limitation of the wind farms in continuous supply of electric power, hydrostatic power storage facilities are used beside wind farms so that the electric power could be stored and fed in a continuous flow into power systems. Due to the gradual exclusion of conventional generators and 5 percent annual load increments, LOLE index was used in order to calculate the amount of the wind power and the capacity of the necessary power storage facility. To this end, LOLE index was calculated for the first year as the reference index for the estimation of the amount of wind power and the capacity of the storage facility in consequent years. For the upcoming years, calculations have been made to account for the gradual exclusion of conventional generators in proportion to load increments. The proposed method has been implemented and simulated on IEEE-RTS test system.

  13. WRI 50: Strategies for Cooling Electric Generating Facilities Utilizing Mine Water

    Energy Technology Data Exchange (ETDEWEB)

    Joseph J. Donovan; Brenden Duffy; Bruce R. Leavitt; James Stiles; Tamara Vandivort; Paul Ziemkiewicz

    2004-11-01

    Power generation and water consumption are inextricably linked. Because of this relationship DOE/NETL has funded a competitive research and development initiative to address this relationship. This report is part of that initiative and is in response to DOE/NETL solicitation DE-PS26-03NT41719-0. Thermal electric power generation requires large volumes of water to cool spent steam at the end of the turbine cycle. The required volumes are such that new plant siting is increasingly dependent on the availability of cooling circuit water. Even in the eastern U.S., large rivers such as the Monongahela may no longer be able to support additional, large power stations due to subscription of flow to existing plants, industrial, municipal and navigational requirements. Earlier studies conducted by West Virginia University (WV 132, WV 173 phase I, WV 173 Phase II, WV 173 Phase III, and WV 173 Phase IV in review) have identified that a large potential water resource resides in flooded, abandoned coal mines in the Pittsburgh Coal Basin, and likely elsewhere in the region and nation. This study evaluates the technical and economic potential of the Pittsburgh Coal Basin water source to supply new power plants with cooling water. Two approaches for supplying new power plants were evaluated. Type A employs mine water in conventional, evaporative cooling towers. Type B utilizes earth-coupled cooling with flooded underground mines as the principal heat sink for the power plant reject heat load. Existing mine discharges in the Pittsburgh Coal Basin were evaluated for flow and water quality. Based on this analysis, eight sites were identified where mine water could supply cooling water to a power plant. Three of these sites were employed for pre-engineering design and cost analysis of a Type A water supply system, including mine water collection, treatment, and delivery. This method was also applied to a ''base case'' river-source power plant, for comparison. Mine

  14. WRI 50: Strategies for Cooling Electric Generating Facilities Utilizing Mine Water

    Energy Technology Data Exchange (ETDEWEB)

    Joseph J. Donovan; Brenden Duffy; Bruce R. Leavitt; James Stiles; Tamara Vandivort; Paul Ziemkiewicz

    2004-11-01

    Power generation and water consumption are inextricably linked. Because of this relationship DOE/NETL has funded a competitive research and development initiative to address this relationship. This report is part of that initiative and is in response to DOE/NETL solicitation DE-PS26-03NT41719-0. Thermal electric power generation requires large volumes of water to cool spent steam at the end of the turbine cycle. The required volumes are such that new plant siting is increasingly dependent on the availability of cooling circuit water. Even in the eastern U.S., large rivers such as the Monongahela may no longer be able to support additional, large power stations due to subscription of flow to existing plants, industrial, municipal and navigational requirements. Earlier studies conducted by West Virginia University (WV 132, WV 173 phase I, WV 173 Phase II, WV 173 Phase III, and WV 173 Phase IV in review) have identified that a large potential water resource resides in flooded, abandoned coal mines in the Pittsburgh Coal Basin, and likely elsewhere in the region and nation. This study evaluates the technical and economic potential of the Pittsburgh Coal Basin water source to supply new power plants with cooling water. Two approaches for supplying new power plants were evaluated. Type A employs mine water in conventional, evaporative cooling towers. Type B utilizes earth-coupled cooling with flooded underground mines as the principal heat sink for the power plant reject heat load. Existing mine discharges in the Pittsburgh Coal Basin were evaluated for flow and water quality. Based on this analysis, eight sites were identified where mine water could supply cooling water to a power plant. Three of these sites were employed for pre-engineering design and cost analysis of a Type A water supply system, including mine water collection, treatment, and delivery. This method was also applied to a ''base case'' river-source power plant, for comparison. Mine

  15. Energy quality in DIFG (Double Fed Induction Generator) in isolated systems; Qualidade de energia de DFIG (Double Fed Induction Generator) em sistemas isolados

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, J.C. [Universidade Federal do ABC (UFABC), Santo Andre, SP (Brazil)], Email: juliocarlos.teixeira@ufabc.edu.br

    2009-07-01

    The paper presents effects that double fed induction generator can have on electrical isolated systems, or with high impedance of the network. The model and results show that the inverter should be designed to prevent that inappropriate frequency tension affect not only the performance of the generator, as well as the power system. The amplitudes of harmonics, as much the tensions generated as the excitation current can be calculated by the proposed equivalent circuit.

  16. Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells

    Directory of Open Access Journals (Sweden)

    Shimpei Gotoh

    2014-09-01

    Full Text Available No methods for isolating induced alveolar epithelial progenitor cells (AEPCs from human embryonic stem cells (hESCs and induced pluripotent stem cells (hiPSCs have been reported. Based on a study of the stepwise induction of alveolar epithelial cells (AECs, we identified carboxypeptidase M (CPM as a surface marker of NKX2-1+ “ventralized” anterior foregut endoderm cells (VAFECs in vitro and in fetal human and murine lungs. Using SFTPC-GFP reporter hPSCs and a 3D coculture system with fetal human lung fibroblasts, we showed that CPM+ cells isolated from VAFECs differentiate into AECs, demonstrating that CPM is a marker of AEPCs. Moreover, 3D coculture differentiation of CPM+ cells formed spheroids with lamellar-body-like structures and an increased expression of surfactant proteins compared with 2D differentiation. Methods to induce and isolate AEPCs using CPM and consequently generate alveolar epithelial spheroids would aid human pulmonary disease modeling and regenerative medicine.

  17. The potential economic impact of constructing and operating solar power generation facilities in Nevada

    Energy Technology Data Exchange (ETDEWEB)

    Schwer, R. K. [Univ. of Nevada, Las Vegas, NV (United States); Riddel, M. [Univ. of Nevada, Las Vegas, NV (United States)

    2004-02-01

    Nevada has a vast potential for electricity generation using solar power. An examination of the stock of renewable resources in Nevada proves that the state has the potential to be a leader in renewable-electric generation--one of the best in the world. This study provides estimates on the economic impact in terms of employment, personal income, and gross state product (GSP) of developing a portion of Nevada's solar energy generation resources.

  18. Measured effect of wind generation on the fuel consumption of an isolated diesel power system

    Science.gov (United States)

    Stiller, P. H.; Scott, G. W.; Shaltens, R. K.

    1983-01-01

    The Block Island Power Company (BIPCO), on Block Island, Rhode Island, operates an isolated electric power system consisting of diesel generation and an experimental wind turbine. The 150-kW wind turbine, designated MOD-OA by the U.S. Department of Energy is typically operated in parallel with two diesel generators to serve an average winter load of 350 kW. Wind generation serves up to 60 percent of the system demand depending on wind speed and total system load. Results of diesel fuel consumption measurements are given for the diesel units operated in parallel with the wind turbine and again without the wind turbine. The fuel consumption data are used to calculate the amount of fuel displaced by wind energy. Results indicate that the wind turbine displaced 25,700 lbs. of the diesel fuel during the test period, representing a calculated reduction in fuel consumption of 6.7 percent while generating 11 percent of the total electric energy. The amount of displaced fuel depends on operating conditions and system load. It is also shown that diesel engine throttle activity resulting from wind gusts which rapidly change the wind turbine output do not significantly influence fuel consumption.

  19. Facile synthesis of corticosteroids prodrugs from isolated hydrocortisone acetate and their quantum chemical calculations

    Science.gov (United States)

    Sethi, Arun; Singh, Ranvijay Pratap; Prakash, Rohit; Amandeep

    2017-02-01

    In the present research paper corticosteroids prodrugs of hydrocortisone acetate (1) have been synthesized, which was isolated from the flowers of Allamanda Violacea. The hydrocortisone acetate (1) was hydrolyzed to hydrocortisone (2) which was subsequently converted to prednisolone (3). Both the hydrocortisone (1) and prednisolone (2) underwent Steglich esterification with naproxen and Ibuprofen yielding compounds 11, 17 dihydroxy-21-(2-(6-methoxynaphthalene-2yl) propionoxy)-pregn-4-ene-3, 20-dione (4), 11, 17-dihydroxy-21-(2-(4-isobutylphenyl) propionoxy)-pregn-4-ene-3, 20-dione (5), 21-(2-(6-methoxynaphthalene-2-yl) propionoxy) 11,17-di-hydroxy-3,20-diketo-pregn-1,4-diene (6) and 11,17-di-hydroxy-3,20-diketo-pregn-1,4-diene-21-yl-2-(4-isobutylphenyl) propanoate (7). The synthesized compounds have been characterized with the help of spectroscopic techniques like 1H, 13C NMR, FT-IR spectroscopy and mass spectrometry. Density functional theory (DFT) with B3LYP functional and 6-31G (d, p) basis set has been used for the Quantum chemical calculations. The electronic properties such as frontier orbitals and band gap energies were calculated by TD-DFT approach. Intramolecular interactions have been identified by AIM (Atoms in Molecule) approach and vibrational wavenumbers have been calculated using DFT method. The reactivity and reactive site within the synthesized prodrugs have been examined with the help of reactivity descriptors. Dipole moment, polarizability and first static hyperpolarizability have been calculated to get a better insight of the properties of synthesized prodrugs. The molecular electrostatic potential (MEP) surface analysis has also been carried out.

  20. Is there ethological isolation among Drosophila melanogaster strains reared for more than 35 generations on different food?

    Directory of Open Access Journals (Sweden)

    Pavković-Lučić Sofija

    2009-01-01

    Full Text Available Ethological isolation among three strains of D. melanogaster reared for more than 35 generations on different food (standard cornmeal-agar-yeast medium, banana, and tomato was tested in a series of multiple choice tests. Long-term maintenance on different substrates did not contribute to sexual isolation among three 'nutritional strains', as degrees of ethological isolation (Malogolowkin-Cohen's isolation indices were not statistically significant. Males and females reared on different nutritional resources were equally successful in matings, i.e., significant differences in the number of copulations achieved by males and females from various lines were not observed.

  1. The Cost-Optimal Distribution of Wind and Solar Generation Facilities in a Simplified Highly Renewable European Power System

    Science.gov (United States)

    Kies, Alexander; von Bremen, Lüder; Schyska, Bruno; Chattopadhyay, Kabitri; Lorenz, Elke; Heinemann, Detlev

    2016-04-01

    The transition of the European power system from fossil generation towards renewable sources is driven by different reasons like decarbonisation and sustainability. Renewable power sources like wind and solar have, due to their weather dependency, fluctuating feed-in profiles, which make their system integration a difficult task. To overcome this issue, several solutions have been investigated in the past like the optimal mix of wind and PV [1], the extension of the transmission grid or storages [2]. In this work, the optimal distribution of wind turbines and solar modules in Europe is investigated. For this purpose, feed-in data with an hourly temporal resolution and a spatial resolution of 7 km covering Europe for the renewable sources wind, photovoltaics and hydro was used. Together with historical load data and a transmission model , a simplified pan-European power power system was simulated. Under cost assumptions of [3] the levelized cost of electricity (LCOE) for this simplified system consisting of generation, consumption, transmission and backup units is calculated. With respect to the LCOE, the optimal distribution of generation facilities in Europe is derived. It is shown, that by optimal placement of renewable generation facilities the LCOE can be reduced by more than 10% compared to a meta study scenario [4] and a self-sufficient scenario (every country produces on average as much from renewable sources as it consumes). This is mainly caused by a shift of generation facilities towards highly suitable locations, reduced backup and increased transmission need. The results of the optimization will be shown and implications for the extension of renewable shares in the European power mix will be discussed. The work is part of the RESTORE 2050 project (Wuppertal Institute, Next Energy, University of Oldenburg), that is financed by the Federal Ministry of Education and Research (BMBF, Fkz. 03SFF0439A). [1] Kies, A. et al.: Kies, Alexander, et al

  2. Review of Catalytic Hydrogen Generation in the Defense Waste Processing Facility (DWPF) Chemical Processing Cell

    Energy Technology Data Exchange (ETDEWEB)

    Koopman, D. C.

    2004-12-31

    This report was prepared to fulfill the Phase I deliverable for HLW/DWPF/TTR-98-0018, Rev. 2, ''Hydrogen Generation in the DWPF Chemical Processing Cell'', 6/4/2001. The primary objective for the preliminary phase of the hydrogen generation study was to complete a review of past data on hydrogen generation and to prepare a summary of the findings. The understanding was that the focus should be on catalytic hydrogen generation, not on hydrogen generation by radiolysis. The secondary objective was to develop scope for follow-up experimental and analytical work. The majority of this report provides a summary of past hydrogen generation work with radioactive and simulated Savannah River Site (SRS) waste sludges. The report also includes some work done with Hanford waste sludges and simulants. The review extends to idealized systems containing no sludge, such as solutions of sodium formate and formic acid doped with a noble metal catalyst. This includes general information from the literature, as well as the focused study done by the University of Georgia for the SRS. The various studies had a number of points of universal agreement. For example, noble metals, such as Pd, Rh, and Ru, catalyze hydrogen generation from formic acid and formate ions, and more acid leads to more hydrogen generation. There were also some points of disagreement between different sources on a few topics such as the impact of mercury on the noble metal catalysts and the identity of the most active catalyst species. Finally, there were some issues of potential interest to SRS that apparently have not been systematically studied, e.g. the role of nitrite ion in catalyst activation and reactivity. The review includes studies covering the period from about 1924-2002, or from before the discovery of hydrogen generation during simulant sludge processing in 1988 through the Shielded Cells qualification testing for Sludge Batch 2. The review of prior studies is followed by a

  3. VITRINE: a next generation low Earth orbit exposure facility for astrochemistry and exobiology

    Science.gov (United States)

    Cottin, Hervé; Coll, P.; Ehrenfreund, P.; Baratta, G. A.; Bénilan, Y.; Bertrand, M.; Borget, F.; Buch, A.; Chieavassa, T.; Couturier, I.; Dartois, E.; D'Hendecourt, L.; Duvernay, F.; Fray, N.; Gazeau, M. C.; Leach, S.; Mattioda, A.; Piétri, N.; Quinn, R.; Raulin, F.; Ricco, A. J.; Santos, O.; Strazzulla, G.; Szopa, C.; Theulé, P.; Westall, F.; Villenave, M.; Lefevre, F.; Belasic, M.; Chazalnoel, P.; Viso, M.

    The study of the evolution of organic matter submitted to space conditions, and more specifi-cally to solar photons in the vacuum ultraviolet range (120-200 nm) has been undertaken in low Earth Orbit since the 90's, and implemented on various space platforms. The most recent facil-ities of exposition are BIOPAN outside Russian automatic capsules FOTON, and EXPOSE-E -R on the International Space Station. They allow the photolysis of many different samples simultaneously, and provide us with valuable data about the formation and evolution of organic matter in the Solar System (meteorites, comets, Titan atmosphere, Martian surface. . . ) and in the Interstellar Medium. They have been used by European teams in the recent past (OR-GANIC on BIOPAN V-FOTON M2, UVolution on BIOPAN VI-FOTON M3 and PROCESS on EXPOSE-E), and some experiments are currently in progress outside of the ISS: AMINO and ORGANICS on EXPOSE-R). These existing tools are very valuable; however they have significant limitations downscaling their capabilities: 1) These facilities are passively placed outside Earth orbiting space modules, with no Sun pointing device. This results in a low integrated time of actual exposition to the Sun (about 10 percents of total time in space). 2) It is not possible to follow the evolution of the samples during its exposition time in space. 3) The temperature of the samples is not controlled. It is impossible to implement experiments on sample at very low temperature (pure ice, or mixtures at about 10k). That kind of experi-ments is very current in the laboratory to study interstellar and cometary ices, or the evolution of the surface of icy satellites of giant planets. Therefore we are proposing the development of a new space instrument, for long duration expositions in space (> 1 yr) with the following features: -Control of temperature, and capacity to deal with samples at very low T. -Sun pointing device or equivalent mechanism (heliostat) in order to optimize the

  4. Isolation and characterization of (15Z)-lycopene thermally generated from a natural source.

    Science.gov (United States)

    Takehara, Munenori; Kuwa, Takahiro; Inoue, Yoshinori; Kitamura, Chitoshi; Honda, Masaki

    2015-11-06

    (15Z)-Lycopene was prepared by thermal isomerization of (all-E)-lycopene derived from tomatoes, and isolated by using a series of chromatographies. The fine red crystalline powder of (15Z)-lycopene was obtained from 556 mg of (all-E)-lycopene with a yield of 0.6 mg (purity: reversed-phase HPLC, 97.2%; normal-phase HPLC, ≥99.9%), and (1)H and (13)C NMR spectra of the isomer were fully assigned. More refined computational analyses that considered differences in the energy levels of the conformers involved in isomerization have also determined the stabilities of (15Z)-lycopene and other geometric isomers, along with the activation energies during isomerization from the all-E form. The fine control of conditions for HPLC separation and an advanced theoretical insight into geometric isomerization have led to the discovery of the 15Z-isomer generated from a natural source.

  5. Doubly Fed Induction Generator Modeling and Scalar Controlled for Supplying an Isolated Site

    Directory of Open Access Journals (Sweden)

    Said Drid

    2006-06-01

    Full Text Available This paper deal with the scalar control of the doubly fed induction generator, (DFIG, supplying an isolated site and using the wind power. The DFIG is more adapted for this application, because even if it receives a variable speed on its rotor shaft, due to variable wind speed, a voltage wave with constants magnitude and frequency can be produced. If the injected rotor currents with the specific voltage/frequency ratio according to rotor variable speed and the fixed frequency and magnitude stator voltage are the known problems, than to solve these latter, a simple scalar control method is proposed taking into account the variable speed condition. Experimental results are given in this work so as to attest the feasibility and the simplicity of our method.

  6. Isolation of circulating tumor cells using a microvortex-generating herringbone-chip.

    Science.gov (United States)

    Stott, Shannon L; Hsu, Chia-Hsien; Tsukrov, Dina I; Yu, Min; Miyamoto, David T; Waltman, Belinda A; Rothenberg, S Michael; Shah, Ajay M; Smas, Malgorzata E; Korir, George K; Floyd, Frederick P; Gilman, Anna J; Lord, Jenna B; Winokur, Daniel; Springer, Simeon; Irimia, Daniel; Nagrath, Sunitha; Sequist, Lecia V; Lee, Richard J; Isselbacher, Kurt J; Maheswaran, Shyamala; Haber, Daniel A; Toner, Mehmet

    2010-10-26

    Rare circulating tumor cells (CTCs) present in the bloodstream of patients with cancer provide a potentially accessible source for detection, characterization, and monitoring of nonhematological cancers. We previously demonstrated the effectiveness of a microfluidic device, the CTC-Chip, in capturing these epithelial cell adhesion molecule (EpCAM)-expressing cells using antibody-coated microposts. Here, we describe a high-throughput microfluidic mixing device, the herringbone-chip, or "HB-Chip," which provides an enhanced platform for CTC isolation. The HB-Chip design applies passive mixing of blood cells through the generation of microvortices to significantly increase the number of interactions between target CTCs and the antibody-coated chip surface. Efficient cell capture was validated using defined numbers of cancer cells spiked into control blood, and clinical utility was demonstrated in specimens from patients with prostate cancer. CTCs were detected in 14 of 15 (93%) patients with metastatic disease (median = 63 CTCs/mL, mean = 386 ± 238 CTCs/mL), and the tumor-specific TMPRSS2-ERG translocation was readily identified following RNA isolation and RT-PCR analysis. The use of transparent materials allowed for imaging of the captured CTCs using standard clinical histopathological stains, in addition to immunofluorescence-conjugated antibodies. In a subset of patient samples, the low shear design of the HB-Chip revealed microclusters of CTCs, previously unappreciated tumor cell aggregates that may contribute to the hematogenous dissemination of cancer.

  7. 40 CFR 268.7 - Testing, tracking, and recordkeeping requirements for generators, treaters, and disposal facilities.

    Science.gov (United States)

    2010-07-01

    ..., and place a copy in their files. Generators of hazardous debris excluded from the definition of... are not limited to case-by-case extensions under § 268.5, disposal in a no-migration unit under § 268.... Waste analysis data (when available) ✔ ✔ ✔ 6. Date the waste is subject to the prohibition ✔ 7....

  8. Indo-Soviet experiment on an MHD generator test section at the Soviet U-O/sub 2/ facility

    Energy Technology Data Exchange (ETDEWEB)

    Ananthapadmanabhan, P.V.; Bapat, A.V.; Das, A.K. (Bhabha Atomic Research Centre, Bombay (India))

    1982-09-01

    This paper summarizes the major results of the joint Indo-Soviet experiment for testing the Indian MHD generator channel section, designed and fabricated at the Bhabha Atomic Research Centre, Bombay, which was carried out at the U-02 facility in Moscow, USSR, in May 1980. The total test duration was 65 hours and included electrophysical tests and life tests under applied electric fields. The main purpose of the tests was to substantiate the physical concepts, computer codes, design features and special processing techniques involved in the development of MHD generators for the Indian pilot plant at Tiruchirapalli. The experimental observations on the phenomena of heat transfer to the walls, gas dynamics in the channel, electrical characteristics of the generator and near-electrode processes including the analysis of arc spots correlate with the theoretical estimates based on present uderstanding of the physical processes occuring in similar MHD generators. The post-operational inspection of the channel section and extensive investigation of materials through microscopic analysis, chemical analysis and x-ray analysis are also reported in this paper. The joint test programme has clearly demonstrated the definite operating capability of the test section and has given sufficient information and encouragement for building better and improved channels for the future.

  9. Some nuclear chemical aspects of medical generator nuclide production at the Los Alamos hot cell facility

    CERN Document Server

    Fassbender, M; Heaton, R C; Jamriska, D J; Kitten, J J; Nortier, F M; Peterson, E J; Phillips, D R; Pitt, L R; Salazar, L L; Valdez, F O; 10.1524/ract.92.4.237.35596

    2004-01-01

    Generator nuclides constitute a convenient tool for applications in nuclear medicine. In this paper, some radiochemical aspects of generator nuclide parents regularly processed at Los Alamos are introduced. The bulk production of the parent nuclides /sup 68/Ge, /sup 82/Sr, /sup 109/Cd and /sup 88/Zr using charged particle beams is discussed. Production nuclear reactions for these radioisotopes, and chemical separation procedures are presented. Experimental processing yields correspond to 80%-98% of the theoretical thick target yield. Reaction cross sections are modeled using the code ALICE-IPPE; it is observed that the model largely disagrees with experimental values for the nuclear processes treated. Radionuclide production batches are prepared 1-6 times yearly for sales. Batch activities range from 40MBq to 75 GBq.

  10. A facile method for isolation of recombinant human apolipoprotein A-I from E. coli.

    Science.gov (United States)

    Ikon, Nikita; Shearer, Jennifer; Liu, Jianfang; Tran, Jesse J; Feng, ShiBo; Kamei, Ayako; Beckstead, Jennifer A; Kiss, Robert S; Weers, Paul M; Ren, Gang; Ryan, Robert O

    2017-03-20

    Apolipoprotein (apo) A-I is the major protein component of high-density lipoprotein (HDL) and plays key roles in the Reverse Cholesterol Transport pathway. In the past decade, reconstituted HDL (rHDL) has been employed as a therapeutic agent for treatment of atherosclerosis. The ability of rHDL to promote cholesterol efflux from peripheral cells has been documented to reduce the size of atherosclerotic plaque lesions. However, development of apoA-I rHDL-based therapeutics for human use requires a cost effective process to generate an apoA-I product that meets "Good Manufacturing Practice" standards. Methods available for production and isolation of unmodified recombinant human apoA-I at scale are cumbersome, laborious and complex. To overcome this obstacle, a streamlined two-step procedure has been devised for isolation of recombinant untagged human apoA-I from E. coli that takes advantage of its ability to re-fold to a native conformation following denaturation. Heat treatment of a sonicated E. coli supernatant fraction induced precipitation of a large proportion of host cell proteins (HCP), yielding apoA-I as the major soluble protein. Reversed-phase HPLC of this material permitted recovery of apoA-I largely free of HCP and endotoxin. Purified apoA-I possessed α-helix secondary structure, formed rHDL upon incubation with phospholipid and efficiently promoted cholesterol efflux from cholesterol loaded J774 macrophages.

  11. Generation and characterization of electron bunches with ramped current profile at the FLASH facility

    Energy Technology Data Exchange (ETDEWEB)

    Piot, P.; /Northern Illinois U. /Fermilab; Behrens, C.; Gerth, C.; /DESY; Lemery, F.; /Northern Illinois U.; Mihalcea, D.; /Fermilab; Vogt, M.; /DESY

    2011-09-01

    We report on the successful generation of electron bunches with current prof les that have a quasi-linear dependency on the longitudinal coordinate. The technique relies on impressing nonlinear correlations in the longitudinal phase space using a linac operating at two frequencies (1.3 and 3.9 GHz) and a bunch compressor. Data taken for various accelerator settings demonstrate the versatility of the method. The produced bunches have parameters well matched to drive high-gradient accelerating field with enhanced transformer ratio in beam-driven accelerators based on sub-mm-sizes dielectric or plasma structures.

  12. DISTRIBUTION COEFICIENTS (KD) GENERATED FROM A CORE SAMPLE COLLECTED FROM THE SALTSTONE DISPOSAL FACILITY

    Energy Technology Data Exchange (ETDEWEB)

    Almond, P.; Kaplan, D.

    2011-04-25

    Core samples originating from Vault 4, Cell E of the Saltstone Disposal Facility (SDF) were collected in September of 2008 (Hansen and Crawford 2009, Smith 2008) and sent to SRNL to measure chemical and physical properties of the material including visual uniformity, mineralogy, microstructure, density, porosity, distribution coefficients (K{sub d}), and chemical composition. Some data from these experiments have been reported (Cozzi and Duncan 2010). In this study, leaching experiments were conducted with a single core sample under conditions that are representative of saltstone performance. In separate experiments, reducing and oxidizing environments were targeted to obtain solubility and Kd values from the measurable species identified in the solid and aqueous leachate. This study was designed to provide insight into how readily species immobilized in saltstone will leach from the saltstone under oxidizing conditions simulating the edge of a saltstone monolith and under reducing conditions, targeting conditions within the saltstone monolith. Core samples were taken from saltstone poured in December of 2007 giving a cure time of nine months in the cell and a total of thirty months before leaching experiments began in June 2010. The saltstone from Vault 4, Cell E is comprised of blast furnace slag, class F fly ash, portland cement, and Deliquification, Dissolution, and Adjustment (DDA) Batch 2 salt solution. The salt solution was previously analyzed from a sample of Tank 50 salt solution and characterized in the 4QCY07 Waste Acceptance Criteria (WAC) report (Zeigler and Bibler 2009). Subsequent to Tank 50 analysis, additional solution was added to the tank solution from the Effluent Treatment Project as well as from inleakage from Tank 50 pump bearings (Cozzi and Duncan 2010). Core samples were taken from three locations and at three depths at each location using a two-inch diameter concrete coring bit (1-1, 1-2, 1-3; 2-1, 2-2, 2-3; 3-1, 3-2, 3-3) (Hansen and

  13. DISTRIBUTION COEFICIENTS (KD) GENERATED FROM A CORE SAMPLE COLLECTED FROM THE SALTSTONE DISPOSAL FACILITY

    Energy Technology Data Exchange (ETDEWEB)

    Almond, P.; Kaplan, D.

    2011-04-25

    Core samples originating from Vault 4, Cell E of the Saltstone Disposal Facility (SDF) were collected in September of 2008 (Hansen and Crawford 2009, Smith 2008) and sent to SRNL to measure chemical and physical properties of the material including visual uniformity, mineralogy, microstructure, density, porosity, distribution coefficients (K{sub d}), and chemical composition. Some data from these experiments have been reported (Cozzi and Duncan 2010). In this study, leaching experiments were conducted with a single core sample under conditions that are representative of saltstone performance. In separate experiments, reducing and oxidizing environments were targeted to obtain solubility and Kd values from the measurable species identified in the solid and aqueous leachate. This study was designed to provide insight into how readily species immobilized in saltstone will leach from the saltstone under oxidizing conditions simulating the edge of a saltstone monolith and under reducing conditions, targeting conditions within the saltstone monolith. Core samples were taken from saltstone poured in December of 2007 giving a cure time of nine months in the cell and a total of thirty months before leaching experiments began in June 2010. The saltstone from Vault 4, Cell E is comprised of blast furnace slag, class F fly ash, portland cement, and Deliquification, Dissolution, and Adjustment (DDA) Batch 2 salt solution. The salt solution was previously analyzed from a sample of Tank 50 salt solution and characterized in the 4QCY07 Waste Acceptance Criteria (WAC) report (Zeigler and Bibler 2009). Subsequent to Tank 50 analysis, additional solution was added to the tank solution from the Effluent Treatment Project as well as from inleakage from Tank 50 pump bearings (Cozzi and Duncan 2010). Core samples were taken from three locations and at three depths at each location using a two-inch diameter concrete coring bit (1-1, 1-2, 1-3; 2-1, 2-2, 2-3; 3-1, 3-2, 3-3) (Hansen and

  14. Project overview and update on WEAVE: the next generation wide-field spectroscopy facility for the William Herschel Telescope

    CERN Document Server

    Dalton, Gavin; Abrams, Don Carlos; Bonifacio, Piercarlo; Aguerri, J Alfonso L; Middleton, Kevin; Benn, Chris; Dee, Kevin; Sayede, Frederic; Lewis, Ian; Pragt, Johannes; Pico, Sergio; Walton, Nic; Rey, Juerg; Prieto, Carlos Allende; Penate, Jose; Lhome, Emilie; Agocs, Tibor; Alonso, Jose; Terrett, David; Brock, Matthew; Gilbert, James; Ridings, Andy; Guinouard, Isabelle; Verheijen, Marc; Tosh, Ian; Rogers, Kevin; Steele, Iain; Stuik, Remko; Tromp, Neils; Jasko, Attila; Kragt, Jan; Lesman, Dirk; Mottram, Chris; Bates, Stuart; Gribbin, Frank; Rodriguez, Luis Fernando; Delgado, Jose Miguel; Martin, Carlos; Cano, Diego; Navarro, Ramon; Irwin, Mike; Lewis, Jim; Solares, Eduardo Gonzales; Mahony, Neil O; Bianco, Andrea; Zurita, Christina; ter Horst, Rik; Molinari, Emilio; Lodi, Marcello; Guerra, Jose; Vallenari, Antonella; Baruffolo, Andrea

    2014-01-01

    We present an overview of and status report on the WEAVE next-generation spectroscopy facility for the William Herschel Telescope (WHT). WEAVE principally targets optical ground-based follow up of upcoming ground-based (LOFAR) and space-based (Gaia) surveys. WEAVE is a multi-object and multi-IFU facility utilizing a new 2-degree prime focus field of view at the WHT, with a buffered pick-and-place positioner system hosting 1000 multi-object (MOS) fibres, 20 integral field units, or a single large IFU for each observation. The fibres are fed to a single spectrograph, with a pair of 8k(spectral) x 6k (spatial) pixel cameras, located within the WHT GHRIL enclosure on the telescope Nasmyth platform, supporting observations at R~5000 over the full 370-1000nm wavelength range in a single exposure, or a high resolution mode with limited coverage in each arm at R~20000. The project is now in the final design and early procurement phase, with commissioning at the telescope expected in 2017.

  15. Final design and progress of WEAVE: the next generation wide-field spectroscopy facility for the William Herschel Telescope

    Science.gov (United States)

    Dalton, Gavin; Trager, Scott; Abrams, Don Carlos; Bonifacio, Piercarlo; Aguerri, J. Alfonso L.; Middleton, Kevin; Benn, Chris; Dee, Kevin; Sayède, Frédéric; Lewis, Ian; Pragt, Johannes; Pico, Sergio; Walton, Nic; Rey, Jeurg; Allende Prieto, Carlos; Peñate, José; Lhome, Emilie; Agócs, Tibor; Alonso, José; Terrett, David; Brock, Matthew; Gilbert, James; Schallig, Ellen; Ridings, Andy; Guinouard, Isabelle; Verheijen, Marc; Tosh, Ian; Rogers, Kevin; Lee, Martin; Steele, Iain; Stuik, Remko; Tromp, Niels; Jaskó, Attila; Carrasco, Esperanza; Farcas, Szigfrid; Kragt, Jan; Lesman, Dirk; Kroes, Gabby; Mottram, Chris; Bates, Stuart; Rodriguez, Luis Fernando; Gribbin, Frank; Delgado, José Miguel; Herreros, José Miguel; Martin, Carlos; Cano, Diego; Navarro, Ramon; Irwin, Mike; Lewis, Jim; Gonzalez Solares, Eduardo; Murphy, David; Worley, Clare; Bassom, Richard; O'Mahoney, Neil; Bianco, Andrea; Zurita, Christina; ter Horst, Rik; Molinari, Emilio; Lodi, Marcello; Guerra, José; Martin, Adrian; Vallenari, Antonella; Salasnich, Bernardo; Baruffolo, Andrea; Jin, Shoko; Hill, Vanessa; Smith, Dan; Drew, Janet; Poggianti, Bianca; Pieri, Mat; Dominquez Palmero, Lillian; Farina, Cecilia

    2016-08-01

    We present the Final Design of the WEAVE next-generation spectroscopy facility for the William Herschel Telescope (WHT), together with a status update on the details of manufacturing, integration and the overall project schedule now that all the major fabrication contracts are in place. We also present a summary of the current planning behind the 5-year initial phase of survey operations. WEAVE will provide optical ground-based follow up of ground-based (LOFAR) and space-based (Gaia) surveys. WEAVE is a multi-object and multi-IFU facility utilizing a new 2-degree prime focus field of view at the WHT, with a buffered pick-and-place positioner system hosting 1000 multi-object (MOS) fibres, 20 integral field units, or a single large IFU for each observation. The fibres are fed to a single (dual-beam) spectrograph, with total of 16k spectral pixels, located within the WHT GHRIL enclosure on the telescope Nasmyth platform, supporting observations at R 5000 over the full 370-1000nm wavelength range in a single exposure, or a high resolution mode with limited coverage in each arm at R 20000. The project is now in the manufacturing and integration phase with first light expected for early of 2018.

  16. Performance Confirmation Strategies for the Waste Isolation Pilot Plant - A Historical Perspective from an Operating Disposal Facility - 12248

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Steve [John Hart and Associate for Sandia National Laboratories, Carlsbad, New Mexico 88220 (United States)

    2012-07-01

    Performance confirmation is an important element of the Waste Isolation Pilot Plant (WIPP) program. Performance confirmation was first used during the early WIPP site characterization phase to focus experimental activities that address the development of probabilistic repository performance models and to address stakeholder assurance needs. The program is currently used to analyze the conditions of the repository and its surroundings to ensure that the basis for the repository's long-term radioactive waste containment predictions is valid. This basis is related to the parameters, assumptions, conceptual and numerical models that are used to predict or validate the potential radioactive waste containment performance of the system. The concept of performance confirmation for the WIPP is one that has evolved since the first repository work was initiated decades ago and plays an important role in assuring adequate repository performance both now and in the long-term. The WIPP mission has progressed from a pilot project to an operational disposal facility and will progress to eventual site closure when disposal operations are completed. Performance confirmation is an important part of each of these progressions. The concept of disposing radioactive waste in a geologic repository today involves a complete understanding of many technical, political, regulatory, societal and economic elements. Many of these elements overlap and solving all relevant issues necessary to site, operate and decommission a disposal facility should be done with knowledge of each element's requirements and impacts. Performance confirmation is one tool that can help to coordinate many of these elements into a program that actively investigates what is thought to be adequately understood about the system and what information is lacking. A performance confirmation program is used to determine ways to challenge and verify those areas that are thought to be understood and to find ways to

  17. Characterization of 618-11 solid waste burial ground, disposed waste, and description of the waste generating facilities

    Energy Technology Data Exchange (ETDEWEB)

    Hladek, K.L.

    1997-10-07

    The 618-11 (Wye or 318-11) burial ground received transuranic (TRTJ) and mixed fission solid waste from March 9, 1962, through October 2, 1962. It was then closed for 11 months so additional burial facilities could be added. The burial ground was reopened on September 16, 1963, and continued operating until it was closed permanently on December 31, 1967. The burial ground received wastes from all of the 300 Area radioactive material handling facilities. The purpose of this document is to characterize the 618-11 solid waste burial ground by describing the site, burial practices, the disposed wastes, and the waste generating facilities. This document provides information showing that kilogram quantities of plutonium were disposed to the drum storage units and caissons, making them transuranic (TRU). Also, kilogram quantities of plutonium and other TRU wastes were disposed to the three trenches, which were previously thought to contain non-TRU wastes. The site burial facilities (trenches, caissons, and drum storage units) should be classified as TRU and the site plutonium inventory maintained at five kilograms. Other fissile wastes were also disposed to the site. Additionally, thousands of curies of mixed fission products were also disposed to the trenches, caissons, and drum storage units. Most of the fission products have decayed over several half-lives, and are at more tolerable levels. Of greater concern, because of their release potential, are TRU radionuclides, Pu-238, Pu-240, and Np-237. TRU radionuclides also included slightly enriched 0.95 and 1.25% U-231 from N-Reactor fuel, which add to the fissile content. The 618-11 burial ground is located approximately 100 meters due west of Washington Nuclear Plant No. 2. The burial ground consists of three trenches, approximately 900 feet long, 25 feet deep, and 50 feet wide, running east-west. The trenches constitute 75% of the site area. There are 50 drum storage units (five 55-gallon steel drums welded together

  18. Electromagnetic Pulses Generated From Laser Target Interactions at Shenguang II Laser Facility

    Science.gov (United States)

    Yang, Jinwen; Li, Tingshuai; Yi, Tao; Wang, Chuanke; Yang, Ming; Yang, Weiming; Liu, Shenye; Jiang, Shaoen; Ding, Yongkun

    2016-10-01

    Significant electromagnetic pulses (EMP) can be generated by the intensive laser irradiating solid targets in inertial confinement fusion (ICF). To evaluate the EMP intensity and distribution in and outside the laser chamber, we designed and fabricated a discone antenna with ultra-wide bands of over 10 GHz. The return loss (S11 parameter) of this antenna was below -10 dB and could even achieve under -30 dB at 3.1 GHz. The EMP intensity in this study at 80 cm and 40 cm away from the target chamber center (TCC) reached 400 kV/m and 2000 kV/m. The current results are expected to offer preliminary information to study physics regarding laser plasma interactions and will also lay experimental foundation for EMI shielding design to protect various diagnostics. supported by the Fundamental Research Funds for the Central Universities of China (No. ZYGX2015J108) and National Natural Science Foundation of China (Nos. 11575166 and 51581140)

  19. Investigation of isolated substorms: Generation conditions and characteristics of different phases

    Science.gov (United States)

    Vorobjev, V. G.; Yagodkina, O. I.; Zverev, V. L.

    2016-11-01

    Characteristics of isolated substorms selected by variations in the 1-min values of the AL index are analyzed. The substorms were divided into several types with respect to the behavior of the Bz component of the interplanetary magnetic field (IMF) during the expansion phase. The probability of observations of substorms associated with the northward turn of the Bz component of IMF was 19%, while the substorms taking place at Bz 30 min) period of the southward IMF and a following sharp turn of the Bz component of IMF before the north was detected. The data suggest that a northward IMF turn is neither a necessary nor sufficient condition for generating substorms. It has been shown for substorms of the both types that the average duration of the southward IMF to moment T 0 and the average intensity of the magnetic perturbation in the maximum are approximately the same and amount to 80 min and-650 nT, respectively. However, for substorms at Bz loading-unloading processes in the magnetosphere in the periods of magnetospheric substorms were investigated with different functions that determine the efficiency of the energy transfer from the solar wind to the magnetosphere. It has been shown that the highest correlation coefficient ( r = 0.84) is observed when the function suggested by Newell et al. (2007) is used. It has been detected that a simple function VB S yields a high correlation coefficient ( r = 0.75).

  20. Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae).

    Science.gov (United States)

    Bonatelli, Isabel A S; Carstens, Bryan C; Moraes, Evandro M

    2015-01-01

    Microsatellite markers (also known as SSRs, Simple Sequence Repeats) are widely used in plant science and are among the most informative molecular markers for population genetic investigations, but the development of such markers presents substantial challenges. In this report, we discuss how next generation sequencing can replace the cloning, Sanger sequencing, identification of polymorphic loci, and testing cross-amplification that were previously required to develop microsatellites. We report the development of a large set of microsatellite markers for five species of the Neotropical cactus genus Pilosocereus using a restriction-site-associated DNA sequencing (RAD-seq) on a Roche 454 platform. We identified an average of 165 microsatellites per individual, with the absolute numbers across individuals proportional to the sequence reads obtained per individual. Frequency distribution of the repeat units was similar in the five species, with shorter motifs such as di- and trinucleotide being the most abundant repeats. In addition, we provide 72 microsatellites that could be potentially amplified in the sampled species and 22 polymorphic microsatellites validated in two populations of the species Pilosocereus machrisii. Although low coverage sequencing among individuals was observed for most of the loci, which we suggest to be more related to the nature of the microsatellite markers and the possible bias inserted by the restriction enzymes than to the genome size, our work demonstrates that an NGS approach is an efficient method to isolate multispecies microsatellites even in non-model organisms.

  1. Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae.

    Directory of Open Access Journals (Sweden)

    Isabel A S Bonatelli

    Full Text Available Microsatellite markers (also known as SSRs, Simple Sequence Repeats are widely used in plant science and are among the most informative molecular markers for population genetic investigations, but the development of such markers presents substantial challenges. In this report, we discuss how next generation sequencing can replace the cloning, Sanger sequencing, identification of polymorphic loci, and testing cross-amplification that were previously required to develop microsatellites. We report the development of a large set of microsatellite markers for five species of the Neotropical cactus genus Pilosocereus using a restriction-site-associated DNA sequencing (RAD-seq on a Roche 454 platform. We identified an average of 165 microsatellites per individual, with the absolute numbers across individuals proportional to the sequence reads obtained per individual. Frequency distribution of the repeat units was similar in the five species, with shorter motifs such as di- and trinucleotide being the most abundant repeats. In addition, we provide 72 microsatellites that could be potentially amplified in the sampled species and 22 polymorphic microsatellites validated in two populations of the species Pilosocereus machrisii. Although low coverage sequencing among individuals was observed for most of the loci, which we suggest to be more related to the nature of the microsatellite markers and the possible bias inserted by the restriction enzymes than to the genome size, our work demonstrates that an NGS approach is an efficient method to isolate multispecies microsatellites even in non-model organisms.

  2. Topical report: Natural convection shutdown heat removal test facility (NSTF) evaluation for generating additional reactor cavity cooling system (RCCS) data.

    Energy Technology Data Exchange (ETDEWEB)

    Farmer, M. T.; Kilsdonk, D. J.; Tzanos, C.P.; Lomperski, S.; Aeschlimann, R.W.; Pointer, D.; Nuclear Engineering Division

    2005-09-01

    As part of the Department of Energy (DOE) Generation IV roadmapping activity, the Very High Temperature gas cooled Reactor (VHTR) has been selected as the principal concept for hydrogen production and other process-heat applications such as district heating and potable water production. On this basis, the DOE has selected the VHTR for additional R&D with the ultimate goal of demonstrating emission-free electricity and hydrogen production with this advanced reactor concept. One of the key passive safety features of the VHTR is the potential for decay heat removal by natural circulation of air in a Reactor Cavity Cooling System (RCCS). The air-cooled RCCS concept is notably similar to the Reactor Vessel Auxiliary Cooling System (RVACS) that was developed for the General Electric PRISM sodium-cooled fast reactor. As part of the DOE R&D program that supported the development of this fast reactor concept, the Natural Convection Shutdown Heat Removal Test Facility (NSTF) was developed at ANL to provide proof-of-concept data for the RVACS under prototypic natural convection flow, temperature, and heat flux conditions. Due to the similarity between RVACS and the RCCS, current VHTR R&D plans call for the utilization of the NSTF to provide RCCS model development and validation data, in addition to supporting design validation and optimization activities. Both air-cooled and water-cooled RCCS designs are to be included. In support of this effort, ANL has been tasked with the development of an engineering plan for mechanical and instrumentation modifications to NSTF to ensure that sufficiently detailed temperature, heat flux, velocity and turbulence profiles are obtained to adequately qualify the codes under the expected range of air-cooled RCCS flow conditions. Next year, similar work will be carried out for the alternative option of a water-cooled RCCS design. Analysis activities carried out in support of this experiment planning task have shown that: (a) in the RCCS, strong

  3. Use of Produced Water in Recirculated Cooling Systems at Power Generating Facilities

    Energy Technology Data Exchange (ETDEWEB)

    C. McGowin; M. DiFilippo; L. Weintraub

    2006-06-30

    Tree ring studies indicate that, for the greater part of the last three decades, New Mexico has been relatively 'wet' compared to the long-term historical norm. However, during the last several years, New Mexico has experienced a severe drought. Some researchers are predicting a return of very dry weather over the next 30 to 40 years. Concern over the drought has spurred interest in evaluating the use of otherwise unusable saline waters to supplement current fresh water supplies for power plant operation and cooling and other uses. The U.S. Department of Energy's National Energy Technology Laboratory sponsored three related assessments of water supplies in the San Juan Basin area of the four-corner intersection of Utah, Colorado, Arizona, and New Mexico. These were (1) an assessment of using water produced with oil and gas as a supplemental supply for the San Juan Generating Station (SJGS); (2) a field evaluation of the wet-surface air cooling (WSAC) system at SJGS; and (3) the development of a ZeroNet systems analysis module and an application of the Watershed Risk Management Framework (WARMF) to evaluate a range of water shortage management plans. The study of the possible use of produced water at SJGS showed that produce water must be treated to justify its use in any reasonable quantity at SJGS. The study identified produced water volume and quality, the infrastructure needed to deliver it to SJGS, treatment requirements, and delivery and treatment economics. A number of produced water treatment alternatives that use off-the-shelf technology were evaluated along with the equipment needed for water treatment at SJGS. Wet surface air-cooling (WSAC) technology was tested at the San Juan Generating Station (SJGS) to determine its capacity to cool power plant circulating water using degraded water. WSAC is a commercial cooling technology and has been used for many years to cool and/or condense process fluids. The purpose of the pilot test was to

  4. Clonally related methicillin-resistant Staphylococcus aureus isolated from short-finned pilot whales (Globicephala macrorhynchus), human volunteers, and a bayfront cetacean rehabilitation facility.

    Science.gov (United States)

    Hower, Suzanne; Phillips, Matthew C; Brodsky, Micah; Dameron, Adrienne; Tamargo, Manuel A; Salazar, Norma C; Jackson, Charlene R; Barrett, John B; Davidson, Maureen; Davis, Johnnie; Mukherjee, Sampa; Ewing, Ruth Y; Gidley, Maribeth L; Sinigalliano, Christopher D; Johns, Lisa; Johnson, Frank E; Adebanjo, Olufunmilola; Plano, Lisa R W

    2013-05-01

    In May of 2011, a live mass stranding of 26 short-finned pilot whales (Globicephala macrorhynchus) occurred in the lower Florida Keys. Five surviving whales were transferred from the original stranding site to a nearby marine mammal rehabilitation facility where they were constantly attended to by a team of volunteers. Bacteria cultured during the routine clinical care of the whales and necropsy of a deceased whale included methicillin-sensitive and methicillin-resistant Staphylococcus aureus (MSSA and MRSA). In order to investigate potential sources or reservoirs of MSSA and MRSA, samples were obtained from human volunteers, whales, seawater, and sand from multiple sites at the facility, nearby recreational beaches, and a canal. Samples were collected on 3 days. The second collection day was 2 weeks after the first, and the third collection day was 2 months after the last animal was removed from the facility. MRSA and MSSA were isolated on each day from the facility when animals and volunteers were present. MSSA was found at an adjacent beach on all three collection days. Isolates were characterized by utilizing a combination of quantitative real-time PCR to determine the presence of mecA and genes associated with virulence, staphylococcal protein A typing, staphylococcal cassette chromosome mec typing, multilocus sequence typing, and pulsed field gel electrophoresis (PFGE). Using these methods, clonally related MRSA were isolated from multiple environmental locations as well as from humans and animals. Non-identical but genetically similar MSSA and MRSA were also identified from distinct sources within this sample pool. PFGE indicated that the majority of MRSA isolates were clonally related to the prototype human strain USA300. These studies support the notion that S. aureus may be shed into an environment by humans or pilot whales and subsequently colonize or infect exposed new hosts.

  5. Efficient generation of isolated attosecond pulses with high beam-quality by two-color Bessel-Gauss beams

    CERN Document Server

    Wang, Zhe; Zhang, Qingbin; Wang, Shaoyi; Lu, Peixiang

    2011-01-01

    The generation of isolated attosecond pulses with high efficiency and high beam quality is essential for attosec- ond spectroscopy. We numerically investigate the supercontinuum generation in a neutral rare-gas medium driven by a two-color Bessel-Gauss beam. The results show that an efficient smooth supercontinuum in the plateau is obtained after propagation, and the spatial profile of the generated attosecond pulse is Gaussian-like with the divergence angle of 0.1 degree in the far field. This bright source with high beam quality is beneficial for detecting and controlling the microscopic processes on attosecond time scale.

  6. The calculation and estimation of wastes generated by decommissioning of nuclear facilities. Tokai works and Ningyo-toge Environmental Engineering Center

    Energy Technology Data Exchange (ETDEWEB)

    Ayame, Y.; Tanabe, T.; Takahashi, K.; Takeda, S. [Japan Nuclear Cycle Development Inst., Tokai Works, Waste Management and Fuel Cycle Research Center, Tokai, Ibaraki (Japan)

    2001-07-01

    This investigation was conducted as a part of planning the low-level radioactive waste management program (LLW management program). The aim of this investigation was contributed to compile the radioactive waste database of JNC's LLW management program. All nuclear facilities of the Tokai works and Ningyo-toge Environmental Engineering Center were investigated in this work. The wastes generated by the decommissioning of each nuclear facility were classified into radioactive waste and others (exempt waste and non-radioactive waste), and the amount of the wastes was estimated. The estimated amounts of radioactive wastes generated by decommissioning of the nuclear facilities are as follows. (1) Tokai works: The amount of waste generated by decommissioning of nuclear facilities of the Tokai works is about 1,079,100 ton. The amount of radioactive waste is about 15,400 ton. The amount of exempt waste and non-radioactive waste is about 1,063,700 ton. (2) Ningyo-toge Environmental Engineering Center: The amount of waste generated by decommissioning of nuclear facilities of Ningyo-toge Environmental Engineering Center is about 112,500 ton. The amount of radioactive waste is about 7,800 ton. The amount of exempt waste and non-radioactive waste is about 104,700 ton. (author)

  7. Computer code analysis of steam generator in thermal-hydraulic test facility simulating nuclear power plant; Ydinvoimalaitosta kuvaavan koelaitteiston hoeyrystimien analysointi tietokoneohjelmilla

    Energy Technology Data Exchange (ETDEWEB)

    Virtanen, E.

    1995-12-31

    In the study three loss-of-feedwater type experiments which were preformed with the PACTEL facility has been calculated with two computer codes. The purpose of the experiments was to gain information about the behaviour of horizontal steam generator in a situation where the water level on the secondary side of the steam generator is decreasing. At the same time data that can be used in the assessment of thermal-hydraulic computer codes was assembled. The purpose of the work was to study the capabilities of two computer codes, APROS version 2.11 and RELAP5/MOD3.1, to calculate the phenomena in horizontal steam generator. In order to make the comparison of the calculation results easier the same kind of model of the steam generator was made for both codes. Only the steam generator was modelled, the rest of the facility was given for the codes as a boundary condition. (23 refs.).

  8. Generation of Bright Isolated Attosecond Soft X-Ray Pulses Driven by Multi-Cycle Mid-Infrared Lasers

    CERN Document Server

    Chen, M -C; Mancuso, C; Dollar, F; Galloway, B; Popmintchev, D; Huang, P -C; Walker, B; Plaja, L; Jaron-Becker, A; Becker, A; Popmintchev, T; Murnane, M M; Kapteyn, H C

    2014-01-01

    High harmonic generation driven by femtosecond lasers makes it possible to capture the fastest dynamics in molecules and materials. However, to date the shortest attosecond (as) pulses have been produced only in the extreme ultraviolet (EUV) region of the spectrum below 100 eV, which limits the range of materials and molecular systems that can be explored. Here we use advanced experiment and theory to demonstrate a remarkable convergence of physics: when mid-infrared lasers are used to drive the high harmonic generation process, the conditions for optimal bright soft X-ray generation naturally coincide with the generation of isolated attosecond pulses. The temporal window over which phase matching occurs shrinks rapidly with increasing driving laser wavelength, to the extent that bright isolated attosecond pulses are the norm for 2 \\mu m driving lasers. Harnessing this realization, we demonstrate the generation of isolated soft X-ray attosecond pulses at photon energies up to 180 eV for the first time, that e...

  9. The PixFEL project: Progress towards a fine pitch X-ray imaging camera for next generation FEL facilities

    Science.gov (United States)

    Rizzo, G.; Batignani, G.; Benkechkache, M. A.; Bettarini, S.; Casarosa, G.; Comotti, D.; Dalla Betta, G.-F.; Fabris, L.; Forti, F.; Grassi, M.; Lodola, L.; Malcovati, P.; Manghisoni, M.; Mendicino, R.; Morsani, F.; Paladino, A.; Pancheri, L.; Paoloni, E.; Ratti, L.; Re, V.; Traversi, G.; Vacchi, C.; Verzellesi, G.; Xu, H.

    2016-07-01

    The INFN PixFEL project is developing the fundamental building blocks for a large area X-ray imaging camera to be deployed at next generation free electron laser (FEL) facilities with unprecedented intensity. Improvement in performance beyond the state of art in imaging instrumentation will be explored adopting advanced technologies like active edge sensors, a 65 nm node CMOS process and vertical integration. These are the key ingredients of the PixFEL project to realize a seamless large area focal plane instrument composed by a matrix of multilayer four-side buttable tiles. In order to minimize the dead area and reduce ambiguities in image reconstruction, a fine pitch active edge thick sensor is being optimized to cope with very high intensity photon flux, up to 104 photons per pixel, in the range from 1 to 10 keV. A low noise analog front-end channel with this wide dynamic range and a novel dynamic compression feature, together with a low power 10 bit analog to digital conversion up to 5 MHz, has been realized in a 110 μm pitch with a 65 nm CMOS process. Vertical interconnection of two CMOS tiers will be also explored in the future to build a four-side buttable readout chip with high density memories. In the long run the objective of the PixFEL project is to build a flexible X-ray imaging camera for operation both in burst mode, like at the European X-FEL, or in continuous mode with the high frame rates anticipated for future FEL facilities.

  10. The PixFEL project: Progress towards a fine pitch X-ray imaging camera for next generation FEL facilities

    Energy Technology Data Exchange (ETDEWEB)

    Rizzo, G., E-mail: giuliana.rizzo@pi.infn.it [Università di Pisa, Dipartimento di Fisica, I-56127 Pisa (Italy); INFN, Sezione di Pisa, I-56127 Pisa (Italy); Batignani, G. [Università di Pisa, Dipartimento di Fisica, I-56127 Pisa (Italy); INFN, Sezione di Pisa, I-56127 Pisa (Italy); Benkechkache, M.A. [Università di Trento, Dipartimento di Ingegneria Industriale, I-38123 Trento (Italy); University Constantine 1, Department of Electronics in the Science and Technology Faculty, I-25017, Constantine (Algeria); Bettarini, S.; Casarosa, G. [Università di Pisa, Dipartimento di Fisica, I-56127 Pisa (Italy); INFN, Sezione di Pisa, I-56127 Pisa (Italy); Comotti, D. [Università di Pavia, Dipartimento di Ingegneria Industriale e dell' Informazione, I-27100 Pavia (Italy); INFN Sezione di Pavia, I-27100 Pavia (Italy); Dalla Betta, G.-F. [Università di Trento, Dipartimento di Ingegneria Industriale, I-38123 Trento (Italy); TIFPA INFN, I-38123 Trento (Italy); Fabris, L. [INFN Sezione di Pavia, I-27100 Pavia (Italy); Università di Bergamo, Dipartimento di Ingegneria e Scienze Applicate, I-24044 Dalmine (Italy); Forti, F. [Università di Pisa, Dipartimento di Fisica, I-56127 Pisa (Italy); INFN, Sezione di Pisa, I-56127 Pisa (Italy); Grassi, M.; Lodola, L.; Malcovati, P. [Università di Pavia, Dipartimento di Ingegneria Industriale e dell' Informazione, I-27100 Pavia (Italy); INFN Sezione di Pavia, I-27100 Pavia (Italy); Manghisoni, M. [INFN Sezione di Pavia, I-27100 Pavia (Italy); Università di Bergamo, Dipartimento di Ingegneria e Scienze Applicate, I-24044 Dalmine (Italy); and others

    2016-07-11

    The INFN PixFEL project is developing the fundamental building blocks for a large area X-ray imaging camera to be deployed at next generation free electron laser (FEL) facilities with unprecedented intensity. Improvement in performance beyond the state of art in imaging instrumentation will be explored adopting advanced technologies like active edge sensors, a 65 nm node CMOS process and vertical integration. These are the key ingredients of the PixFEL project to realize a seamless large area focal plane instrument composed by a matrix of multilayer four-side buttable tiles. In order to minimize the dead area and reduce ambiguities in image reconstruction, a fine pitch active edge thick sensor is being optimized to cope with very high intensity photon flux, up to 10{sup 4} photons per pixel, in the range from 1 to 10 keV. A low noise analog front-end channel with this wide dynamic range and a novel dynamic compression feature, together with a low power 10 bit analog to digital conversion up to 5 MHz, has been realized in a 110 μm pitch with a 65 nm CMOS process. Vertical interconnection of two CMOS tiers will be also explored in the future to build a four-side buttable readout chip with high density memories. In the long run the objective of the PixFEL project is to build a flexible X-ray imaging camera for operation both in burst mode, like at the European X-FEL, or in continuous mode with the high frame rates anticipated for future FEL facilities.

  11. Isolated attosecond pulses generation from coherent superposition state of helium ion in static electric fields and spatial nonhomogeneous fields

    Science.gov (United States)

    Liu, Hao; Zhang, Zhengzhong; Wu, Yangjiang; Jiang, Shicheng; Yu, Chao

    2016-09-01

    We present a systematic study of high-order harmonic generation (HHG) from helium ion with the initial state prepared as a coherent superposition of electronic ground state and an excited state. As a result, the conversion efficiency of the harmonic spectrum is significantly enhanced. When we add a static electric field in fundamental field, the supercontinuum region of the harmonic spectrum is distinctly extended and an isolated 100 as pulse can be generated. Moreover, we use a spatial nonhomogeneous field to increase the cutoff energy in high-order harmonic generation spectrum, which can be extended to about 700 eV, and an isolated 50 as pulse can be obtained directly by the superposition of the supercontinuum harmonics.

  12. [Genetic analysis of multidrug-resistant Streptococcus pneumoniae including meropenem resistance that was isolated from elderly residents with pneumonia in nursing-care facilities].

    Science.gov (United States)

    Ota, Kazuko; Chiba, Naoko; Sato, Kentaro; Nara, Syoetu; Kato, Satoko; Kanazawa, Hisao; Ikejima, Shin; Takahashi, Yoshihiro; Iwata, Satoshi; Ubukata, Kimiko

    2014-07-01

    From February to December 20XX, penicillin-resistant Streptococcus pneumoniae (PRSP) showing MICs of 16-32 microg/mL to cefotaxime (CTX) and 4-8 microg/mL to meropenem (MEPM) were isolated from 6 patients hospitalized at the general hospital S (2 cases) and hospital A (4 cases), close to the hospital S. Five elderly patients among these six cases came from nursing care facilities or nursing care-related medical facilities. All elderly persons (mean age: 81.7 years) were diagnosed as having pneumonia at the time of admission and the problematic PRSP was isolated from sputum samples collected on admission. Notably, all of these PRSP isolates simultaneously showed high resistance to macrolide agents mediated by an erm (B) gene and to fluoroquinolone agents via mutations in the gyrA and parC genes. Eventually, they were identified as multidrug-resistant S. pneumoniae (MDRSP) with high resistance to many agents. The capsule type of all strains was serotype 19F and multilocus sequence typing (MLST) revealed that they belonged to clonal complex (CC) 7993, which has not been reported before. It was thus concluded that the MDRSP that had spread within the nursing facilities was transmitted to the general hospitals via the elderly inpatients with pneumonia caused by these agents. Although one case finally had a poor outcome, the pneumococcal infection was not the direct trigger of the event. The current ratio of MDRSP is concluded to be very low. However, general hospitals that accept patients for therapeutic purposes from nursing-care facilities have to share epidemiological information in a timely manner with the nursing homes to prevent nosocomial infections.

  13. Isolation

    DEFF Research Database (Denmark)

    Agerholm, Frank Juul

    2011-01-01

    Næringsstoffet har i dette nummer sat fokus på ”velvære i vinterkulden”, ”indendørsaktiviteter” og ”fedtafgift”. I klummen vises det, at disse tre fokusområder, der for en umiddelbar betragtning måske nok synes noget uensartede, falder sammen i ét tema: Isolation!......Næringsstoffet har i dette nummer sat fokus på ”velvære i vinterkulden”, ”indendørsaktiviteter” og ”fedtafgift”. I klummen vises det, at disse tre fokusområder, der for en umiddelbar betragtning måske nok synes noget uensartede, falder sammen i ét tema: Isolation!...

  14. Photovoltaic power generation field test at Hikarigaoka. Sporting facility in a standard district; Hikarigaoka Sporting Facility (Nerima Ward, Tokyo); Tokyo Hikarigaoka taiyoko hatsuden system field test jigyo (hyojun chiku no sports shisetsu). Tokyo Hikarigaoka sports shisetsu

    Energy Technology Data Exchange (ETDEWEB)

    Maeda, Y. [Maeda Corp., Tokyo (Japan)

    1997-05-30

    A 20kW photovoltaic power generation system is mounted on the rooftop of the Hikarigaoka sporting facility building belonging in the large complex J-City consisting of an office building, hotel building, large-space atrium for common use, and the said sporting facility building. The power generated by the system is connected to the power distribution system for the facility, and is supplied to air conditioners, power-driven equipment, and lighting systems in the sporting facility building. Verification is carried out of the compatibility and stability of the photovoltaic power generation system as applied to meet the above-said purposes, and data are collected. In fiscal 1996, a protective glass pane was broken in a solar cell panel, but the operation was not affected and the field test was conducted smoothly. The glass was broken when a tiny defect produced in the manufacturing process grew larger due to thermal stress in the sunshine for the eventual breakdown. When a system is to be installed, upon verification of its practicability, in a building to be built in a place wherein plural buildings exist adjacent to each other, the system installation plan may be incorporated into the building as early as in its construction planning process, and this will reduce the system constructing cost. 1 fig., 1 tab.

  15. Principal component analysis of soft x-ray signals generated by the PF-1000 facility in experiments with solid targets

    Science.gov (United States)

    Kowalska-Strzęciwilk, Ewa; Skrzeczanowski, Wojciech; Czarnecka, Agata; Kubkowska, Monika; Paduch, Marian; Zielińska, Ewa

    2014-05-01

    The paper presents the analysis of soft x-ray signals generated in the PF-1000 facility equipped with a modified inner electrode with a central tungsten insert of 50 mm diameter in experiments with tungsten and carbon samples. The PF-1000 machine was operated with pure deuterium filling under the initial pressure of 1.3 hPa. The machine was powered using a condenser bank charged initially to 24 kV, corresponding to the stored energy of 380 kJ, with the maximum discharge current amounted to 1.8 MA. For investigation of plasma stream-sample interactions, we applied 16-frame laser interferometry, optical spectroscopy and soft x-ray measurements with the use of a system of four silicon pin-diodes. In this paper, we mainly focus on the principal component analysis (PCA) of the registered x-ray signals to find a corelation between the neutron yield and observed maxima in signals. X-ray signals collected by four pin-diodes covered a 9 cm range in front of the electrode ends. Each diode collected a signal from the circle of 3 cm diameter. The presented PCA analysis is based on 57 PF discharges and 16 parameters are taken into account in the analysis. The study of signals from the pin-diode system showed good correlation between the neutron yield and the maximum in the x-ray signal, which appeared about 1000-1300 ns after the maximum of plasma compression.

  16. First diffraction topographic results at a third generation synchrotron radiation facility: application to crystals under an electric field

    Energy Technology Data Exchange (ETDEWEB)

    Rejmankova, P. [European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex (France); Baruchel, J. [European Synchrotron Radiation Facility, BP 220, 38043 Grenoble Cedex (France)

    1995-05-01

    Diffraction topographic techniques allow the observation of inhomogeneities (defects, domains,..) within a single crystal. The unique capabilities of third generation synchrotron radiation facilities, like the ESRF, add new possibilities to these techniques: (1) the exposure time to record a topograph can be reduced to similar 10{sup -2} s, i.e. a factor 10{sup 2}-10{sup 3} below the present standards; (2) an improved resolution associated with the small source size, even when setting the film far ( similar 60 cm) from the sample, allows the size of the sample environment devices to be increased; (3) high sensitivity to small ({>=}10{sup -7}) distortions (misorientations and/or lattice parameter variations); (4) investigation of thick or heavy materials, which were up to now the exclusive field of neutron diffraction. We briefly describe the future Topography and High Resolution Diffraction beamline ID 19, which is designed to optimize the required experimental conditions, and which will be operational at the end of 1995. The first topographic white beam experiments which illustrate these new possibilities were carried out on two (ID11 and D5) of the presently operational beamlines of the ESRF. In this work, particular attention is dedicated to observations performed on crystals under an electric field ({alpha}-LiIO{sub 3}, KTP, LiNbO{sub 3}). Very interesting and encouraging results were obtained, particularly when using ``section`` topography. (orig.).

  17. The precipitation products generation chain for the EUMETSAT Hydrological Satellite Application Facility at C.N.M.C.A.

    Science.gov (United States)

    Biron, Daniele; Melfi, Davide; Zauli, Francesco

    2008-08-01

    The EUMETSAT Satellite Application Facility in support to Hydrology (H-SAF) focuses on development of new geophysical products on precipitation, soil moisture and snow parameters and the utilisation of these parameters in hydrological models, NWP models and water management. The development phase of the H-SAF started in September 2005 under the leadership of Italian Meteorological Service. The "Centro Nazionale di Meteorologia e Climatologia Aeronautica (C.N.M.C.A.)", the Italian National Weather Centre, that physically hosts the generation chain of precipitation products, carried on activities to reach the final target: development of algorithms, validation of results, implementation of operative procedure to supply the service and to monitor the service performances. The paper shows the architectural status of the H-SAF precipitation group and stress the component of operations. It is shown the full correspondence with the EUMETSAT approved H-SAF documents, in particular the Algorithm Theoretical Design Document (ATDD), where products characteristics are referenced. Are also reported the first results, produced during the first H-SAF Workshop, held in Rome in October 2007, of validation activities performed on version 1 products, and last results of products distribution to beta-users in preparation of distributing version 2.

  18. Diverse Geno- and Phenotypes of Persistent Listeria monocytogenes Isolates from Fermented Meat Sausage Production Facilities in Portugal ▿

    Science.gov (United States)

    Ferreira, V.; Barbosa, J.; Stasiewicz, M.; Vongkamjan, K.; Moreno Switt, A.; Hogg, T.; Gibbs, P.; Teixeira, P.; Wiedmann, M.

    2011-01-01

    The persistence of Listeria monocytogenes in food-associated environments represents a key factor in transmission of this pathogen. To identify persistent and transient strains associated with production of fermented meat sausages in northern Portugal, 1,723 L. monocytogenes isolates from raw material and finished products from 11 processors were initially characterized by random amplification of polymorphic DNA (RAPD), PCR-based molecular serotyping, and epidemic clone characterization, as well as cadmium, arsenic, and tetracycline resistance typing. Pulsed-field gel electrophoresis (PFGE) typing of 240 representative isolates provided evidence for persistence of L. monocytogenes for periods of time ranging from 10 to 32 months for all seven processors for which isolates from different production dates were available. Among 50 L. monocytogenes isolates that included one representative for each PFGE pattern obtained from a given sample, 12 isolates showed reduced invasion efficiency in Caco-2 cells, including 8 isolates with premature stop codons in inlA. Among 41 isolates representing sporadic and persistent PFGE types, 22 isolates represented lysogens. Neither strains with reduced invasion nor lysogens were overrepresented among persistent isolates. While the susceptibility of isolates to lysogenic phages also did not correlate with persistence, it appeared to be associated with molecular serotype. Our data show the following. (i) RAPD may not be suitable for analysis of large sets of L. monocytogenes isolates. (ii) While a large diversity of L. monocytogenes subtypes is found in Portuguese fermented meat sausages, persistence of L. monocytogenes in this food chain is common. (iii) Persistent L. monocytogenes strains are diverse and do not appear to be characterized by unique genetic or phenotypic characteristics. PMID:21378045

  19. Microbial gas generation under expected Waste Isolation Pilot Plant repository conditions

    Energy Technology Data Exchange (ETDEWEB)

    Francis, A.J.; Gillow, J.B.; Giles, M.R. [Brookhaven National Lab., Upton, NY (United States). Dept. of Applied Science

    1997-03-01

    Gas generation from the microbial degradation of the organic constituents of transuranic waste under conditions expected at the Waste Isolation Pilot Plant (WIPP) repository was investigated at Brookhaven National Laboratory. The biodegradation of mixed cellulosics (various types of paper) and electron-beam irradiated plastic and rubber materials (polyethylene, polyvinylchloride, neoprene, hypalon, and leaded hypalon) was examined. The rate of gas production from cellulose biodegradation in inundated samples incubated for 1,228 days at 30 C was biphasic, with an initial rapid rate up to approximately 600 days incubation, followed by a slower rate. The rate of total gas production in anaerobic samples containing mixed inoculum was as follows: 0.002 mL/g cellulose/day without nutrients; 0.004 mL/g cellulose/day with nutrients; and 0.01 mL/g cellulose/day in the presence of excess nitrate. Carbon dioxide production proceeded at a rate of 0.009 {micro}mol/g cellulose/day in anaerobic samples without nutrients, 0.05 {micro}mol/g cellulose/day in the presence of nutrients, and 0.2 {micro}mol/g cellulose/day with excess nitrate. Adding nutrients and excess nitrate stimulated denitrification, as evidenced by the accumulation of N{sub 2}O in the headspace (200 {micro}mol/g cellulose). The addition of the potential backfill bentonite increased the rate of CO{sub 2} production to 0.3 {micro}mol/g cellulose/day in anaerobic samples with excess nitrate. Analysis of the solution showed that lactic, acetic, propionic, butyric, and valeric acids were produced due to cellulose degradation. Samples incubated under anaerobic humid conditions for 415 days produced CO{sub 2} at a rate of 0.2 {micro}mol/g cellulose/day in the absence of nutrients, and 1 {micro}mol/g cellulose/day in the presence of bentonite and nutrients. There was no evidence of biodegradation of electron-beam irradiated plastic and rubber.

  20. Hydrogen generation by metal corrosion in simulated Waste Isolation Pilot Plant environments. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Telander, M.R.; Westerman, R.E. [Battelle Pacific Northwest Lab., Richland, WA (United States)

    1997-03-01

    The corrosion and gas-generation characteristics of four material types: low-carbon steel (the current waste packaging material for the Waste Isolation Pilot Plant), Cu-base and Ti-base (alternative packaging) materials, and Al-base (simulated waste) materials were determined in both the liquid and vapor phase of Brine A, a brine representative of an intergranular Salado Formation brine. Test environments consisted primarily of anoxic brine with overpressures of N{sub 2}, CO{sub 2}, H{sub 2}S, and H{sub 2}. Limited tests of low-carbon steel were also performed in simulated-backfill environments and in brine environments with pH values ranging from 3 to 11. Low-carbon steel reacted at a slow, measurable rate with anoxic brine, liberating H{sub 2} on an equimolar basis with Fe reacted. Presence of CO{sub 2} caused the initial reaction to proceed more rapidly, but CO{sub 2}-induced passivation stopped the reaction if the CO{sub 2} were present in sufficient quantities. Addition of H{sub 2}S to a CO{sub 2}-passivated system caused reversal of the passivation. Low-carbon steel immersed in brine with H{sub 2}S showed no reaction, apparently because of passivation of the steel by formation of FeS. Addition of CO{sub 2} to an H{sub 2}S-passivated system did not reverse the passivation. Cu- and Ti-base materials showed essentially no corrosion when exposed to brine and overpressures of N{sub 2}, CO{sub 2}, and H{sub 2}S except for the rapid and complete reaction between Cu-base materials and H{sub 2}S. The Al-base materials reacted at approximately the same rate as low-carbon steel when immersed in anoxic Brine A; considerably more rapidly in the presence of CO{sub 2} or H{sub 2}S; and much more rapidly when iron was present in the system as a brine contaminant. High-purity Al was much more susceptible to corrosion than the 6061 alloy. No significant reaction took place on any material in any environment in the vapor-phase exposures.

  1. A performance assessment of a base isolation system for an emergency diesel generator in a nuclear power plant

    Energy Technology Data Exchange (ETDEWEB)

    Choun, Young Sun; Kim, Min Kyu [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2008-06-15

    This study evaluates the performance of a coil spring-viscous damper system for the vibration and seismic isolation of an Emergency Diesel Generator (EDG) by measuring its operational vibration and seismic responses. The vibration performance of a coil spring-viscous damper system was evaluated by the vibration measurements for an identical EDG set with different base systems - one with an anchor bolt system and the other with a coil spring-viscous damper system. The seismic performance of the coil spring-viscous damper system was evaluated by seismic tests with a scaled model of a base-isolated EDG on a shaking table. The effects of EDG base isolation on the fragility curve and core damage frequency in a nuclear power plant were also investigated through a case study.

  2. Thermal Analysis of Next-Generation Space Telescope (NGST) Mirrors During Optical Testing in the X-Ray Calibration Facility (XRCF)

    Science.gov (United States)

    Page, Tim; Sutherlin, Steven

    2002-01-01

    This paper presents Thermal Analysis of the Next Generation Space Telescope (NGST) Mirrors During Optical Testing in the X-Ray Calibration Facility (XRCF). The contents include: 1) NGST Spacecraft Concept; 2) NGST Mirror Development Testing; 3) NGST Development Mirror; 4) Knudsen Number; 5) Free-Molecular Conduction; 6) Accomodation Coefficient; and 7) Results and Recommendations. This paper is presented in viewgraph form.

  3. Thermal Analysis of Next-Generation Space Telescope (NGST) Mirrors During Optical Testing in the X-Ray Calibration Facility (XRCF)

    Science.gov (United States)

    Page, Tim; Sutherlin, Steven

    2002-07-01

    This paper presents Thermal Analysis of the Next Generation Space Telescope (NGST) Mirrors During Optical Testing in the X-Ray Calibration Facility (XRCF). The contents include: 1) NGST Spacecraft Concept; 2) NGST Mirror Development Testing; 3) NGST Development Mirror; 4) Knudsen Number; 5) Free-Molecular Conduction; 6) Accomodation Coefficient; and 7) Results and Recommendations. This paper is presented in viewgraph form.

  4. Characterization of Electricity Generated by Soil in Microbial Fuel Cells and the Isolation of Soil Source Exoelectrogenic Bacteria

    Directory of Open Access Journals (Sweden)

    Yun-Bin Jiang

    2016-11-01

    Full Text Available Soil has been used to generate electrical power in microbial fuel cells (MFCs and exhibited several potential applications. This study aimed to reveal the effect of soil properties on the generated electricity and the diversity of soil source exoelectrogenic bacteria. Seven soil samples were collected across China and packed into air-cathode MFCs to generate electricity over a 270 d period. The Fe(III-reducing bacteria in soil were enriched and sequenced by Illumina pyrosequencing. Culturable strains of Fe(III-reducing bacteria were isolated and identified phylogenetically. Their exoelectrogenic ability was evaluated by polarization measurement. The results showed that soils with higher organic carbon content but lower soil pH generated higher peak voltage and charge. The sequencing of Fe(III-reducing bacteria showed that Clostridia were dominant in all soil samples. At the family level, Clostridiales Family XI. incertae sedis were dominant in soils with lower organic carbon content but higher pH (>8, while Clostridiaceae, Lachnospiraceae and Planococcaceae were dominant in soils with higher organic carbon content but lower pH. The isolated culturable strains were allied phylogenetically to fifteen different species, of which eleven were Clostridium. The others were Robinsoniella peoriensis, Hydrogenoanaerobacterium saccharovorans, Eubacterium contortum and Oscillibacter ruminantium. The maximum power density generated by the isolates in the MFCs ranged from 16.4 to 28.6 mW m-2. We concluded that soil organic carbon content had the most important effect on power generation and that the Clostridiaceae were the dominant exoelectrogenic bacterial group in soil. This study might lead to the discovery of more soil source exoelectrogenic bacteria species.

  5. Screening of natural yeast isolates under the effects of stresses associated with second-generation biofuel production.

    Science.gov (United States)

    Dubey, Rajni; Jakeer, Shaik; Gaur, Naseem A

    2016-05-01

    Robust microorganisms are required for sustainable second-generation biofuel production. We evaluated the growth and fermentation performance of six natural isolates that were derived from grape wine and medicinal herbs using a wide range of carbon sources, rice and wheat straw hydrolysates as well as stress conditions associated with second-generation ethanol production. Sequence analysis of the 5.8S internal transcribed spacer (ITS) and species-specific PCR amplification of the HO gene region assigned the natural isolates to Saccharomyces cerevisiae. Restriction fragment length polymorphism (RFLP) analysis of the mitochondrial DNA revealed that natural yeast isolates are genetically closer to the laboratory strain BY4741 than to the CEN.PK strains. Dextrose fermentation by a natural isolate, MTCC4780, under semi-anaerobic conditions produced maximum ethanol yields of 0.44 g/g and 0.39 g/g, respectively, with and without the stresses encountered during lignocellulosic ethanol fermentation. However, MTCC4780 produced ethanol yields of 0.48 g/g, 0.42 g/g and 0.45 g/g, respectively, with glucose, rice and wheat straw enzymatic hydrolysate fermentation in a bioreactor. The isolates MTCC4781 and MTCC4796 showed higher growth and fermentation performance than did MTCC4780 in the presence of elevated temperature and pre-treatment inhibitors. Taken together, the MTCC4780, MTCC4781 and MTCC4796 strains have the potential to serve as a platform for lignocellulosic ethanol production under stresses associated with second-generation biofuel production.

  6. Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies

    DEFF Research Database (Denmark)

    Ditzel, Henrik

    2009-01-01

    A method is described for affinity isolation of antigen-specific circulating B cells of interest for subsequent generation of immune antibody phage display libraries. This approach should overcome the problem of low yields of monoclonal antibodies of interest in the libraries generated from...... peripheral blood lymphocytes caused by the low abundance of antigen-specific B cells in the circulation. The preselection of B cells is based on the specificity of the surface Ig receptor and is accomplished using the antigen of interest conjugated to magnetic beads. This method should significantly increase...

  7. Isolation of Aneuploid-Generating Mutants of ASPERGILLUS NIDULANS, One of Which Is Defective in Interphase of the Cell Cycle

    OpenAIRE

    Upshall, A; Mortimore, I. D.

    1984-01-01

    A method is described for isolating mutants potentially defective in loci involved in mitotic chromosome segregation. Conditional lethal, heat-sensitive (42°) mutants were assayed at a subrestrictive temperature of 37° for an inflated production of colonies displaying phenotypes and behavior patterns of whole chromosome aneuploids. Of 14 mutants, three showed specificity for one disomic phenotype, whereas 11 generated colonies mosaic for different aneuploid phenotypes. This latter group is de...

  8. Installation of an on-site power generation facility using gas as a fuel used as an emergency power supply for fire fighting facilities; Shoboyo setsubinado no hijo dengen ni shiyosuru gas wo nenryo to suru jika hatsuden setsubi no donyu

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, K. [Fire Defence Agency, Tokyo (Japan)

    1995-02-01

    This paper summarizes provisions in the Fire Service Law when an on-site power generation facility using gas as a fuel is used as an emergency power supply for fire fighting facilities. The provisions described include the following: regulations on output, number of facilities, operation systems and reserve fuels when installing a power generation facility burning gas exclusively used for normal power use and disaster prevention (a power generation facility using gaseous fuel to be used as a normal and emergency power supply in fire fighting facilities), precautions on operation of these facilities, and evaluation on assurance of stable supply source of the main fuel when no installation of the reserve fuel supply system is required. These regulations take the view of the current status of increase in the structures subjected to fire fighting activities that have installed on-site power generation facilities and cogeneration plants used as ordinary power supply systems. The regulations have become enforced based on the result of discussions on the said power generation facilities burning gas exclusively by the discussion committee for the on-site power generation facilities used as power supply systems for fire fighting facilities in the Nippon Engine Generator Association. 2 figs.

  9. “Silent” Dissemination of Klebsiella pneumoniae Isolates Bearing K. pneumoniae Carbapenemase in a Long-term Care Facility for Children and Young Adults in Northeast Ohio

    Science.gov (United States)

    Viau, Roberto A.; Hujer, Andrea M.; Marshall, Steven H.; Perez, Federico; Hujer, Kristine M.; Briceño, David F.; Dul, Michael; Jacobs, Michael R.; Grossberg, Richard; Toltzis, Philip

    2012-01-01

    Background. Klebsiella pneumoniae isolates harboring the K. pneumoniae carbapenemase gene (blaKPC) are creating a significant healthcare threat in both acute and long-term care facilities (LTCFs). As part of a study conducted in 2004 to determine the risk of stool colonization with extended-spectrum cephalosporin-resistant gram-negative bacteria, 12 isolates of K. pneumoniae that exhibited nonsusceptibility to extended-spectrum cephalosporins were detected. All were gastrointestinal carriage isolates that were not associated with infection. Methods. Reassessment of the carbapenem minimum inhibitory concentrations using revised 2011 Clinical Laboratory Standards Institute breakpoints uncovered carbapenem resistance. To further investigate, a DNA microarray assay, PCR-sequencing of bla genes, immunoblotting, repetitive-sequence-based PCR (rep-PCR) and multilocus sequence typing (MLST) were performed. Results. The DNA microarray detected blaKPC in all 12 isolates, and blaKPC-3 was identified by PCR amplification and sequencing of the amplicon. In addition, a blaSHV-11 gene was detected in all isolates. Immunoblotting revealed “low-level” production of the K. pneumoniae carbapenemase, and rep-PCR indicated that all blaKPC-3-positive K. pneumoniae strains were genetically related (≥98% similar). According to MLST, all isolates belonged to sequence type 36. This sequence type has not been previously linked with blaKPC carriage. Plasmids from 3 representative isolates readily transferred the blaKPC-3 to Escherichia coli J-53 recipients. Conclusions. Our findings reveal the “silent” dissemination of blaKPC-3 as part of Tn4401b on a mobile plasmid in Northeast Ohio nearly a decade ago and establish the first report, to our knowledge, of K. pneumoniae containing blaKPC-3 in an LTCF caring for neurologically impaired children and young adults. PMID:22492318

  10. Molecular epidemiology of extended-spectrum beta-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006.

    Science.gov (United States)

    Fang, Hong; Ataker, Ferda; Hedin, Göran; Dornbusch, Kathrine

    2008-02-01

    The genetic characteristics and molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) among Escherichia coli isolates were investigated at a general hospital and its associated health care facilities in Stockholm, Sweden, during the period from 2001 to 2006. Of 87 consecutive nonduplicate ESBL-positive isolates, 80 isolates encoded CTX-M-type ESBLs, 64 of which were group 1 enzymes. TEM-type and OXA-type beta-lactamases were encoded in 63 and 59% of the ESBL isolates, respectively. Pulsed-field gel electrophoresis (PFGE) analysis revealed 40 different pulsotypes, consisting of 11 clones accounting for 66% of all isolates, and 29 unique patterns. Moreover, of the 11 clones, clones 1 and 4 comprised half of the clonally related isolates (28 of 57). Clone 1 was a persistent endemic clone in the area throughout the years, and clone 4 emerged in 2003. However, in recent years, clone 1 isolates were no longer predominant and were gradually replaced by new emerging strains. Concerning beta-lactamase gene profiles in relation to PFGE pulsotypes, clone-related bla profiles were observed in certain clones, while in most cases different bla profiles could be observed in the same clone, and the same bla profile could be present in different clones. The molecular epidemiology of ESBL-positive E. coli in the area shows shifts in predominant strains and increased clonal diversity over time. The study also indicated that both clonal spread of epidemic strains and transfer of transposable genetic elements might contribute to the proliferation of ESBLs.

  11. Automatic generation of smart earthquake-resistant building system: Hybrid system of base-isolation and building-connection.

    Science.gov (United States)

    Kasagi, M; Fujita, K; Tsuji, M; Takewaki, I

    2016-02-01

    A base-isolated building may sometimes exhibit an undesirable large response to a long-duration, long-period earthquake ground motion and a connected building system without base-isolation may show a large response to a near-fault (rather high-frequency) earthquake ground motion. To overcome both deficiencies, a new hybrid control system of base-isolation and building-connection is proposed and investigated. In this new hybrid building system, a base-isolated building is connected to a stiffer free wall with oil dampers. It has been demonstrated in a preliminary research that the proposed hybrid system is effective both for near-fault (rather high-frequency) and long-duration, long-period earthquake ground motions and has sufficient redundancy and robustness for a broad range of earthquake ground motions.An automatic generation algorithm of this kind of smart structures of base-isolation and building-connection hybrid systems is presented in this paper. It is shown that, while the proposed algorithm does not work well in a building without the connecting-damper system, it works well in the proposed smart hybrid system with the connecting damper system.

  12. Automatic generation of smart earthquake-resistant building system: Hybrid system of base-isolation and building-connection

    Directory of Open Access Journals (Sweden)

    M. Kasagi

    2016-02-01

    Full Text Available A base-isolated building may sometimes exhibit an undesirable large response to a long-duration, long-period earthquake ground motion and a connected building system without base-isolation may show a large response to a near-fault (rather high-frequency earthquake ground motion. To overcome both deficiencies, a new hybrid control system of base-isolation and building-connection is proposed and investigated. In this new hybrid building system, a base-isolated building is connected to a stiffer free wall with oil dampers. It has been demonstrated in a preliminary research that the proposed hybrid system is effective both for near-fault (rather high-frequency and long-duration, long-period earthquake ground motions and has sufficient redundancy and robustness for a broad range of earthquake ground motions.An automatic generation algorithm of this kind of smart structures of base-isolation and building-connection hybrid systems is presented in this paper. It is shown that, while the proposed algorithm does not work well in a building without the connecting-damper system, it works well in the proposed smart hybrid system with the connecting damper system.

  13. Hybrid power technology for remote military facilities

    Energy Technology Data Exchange (ETDEWEB)

    Chapman, R.N.

    1996-09-01

    The Department of Defense (DoD) operates hundreds of test, evaluation, and training facilities across the US and abroad. Due to the nature of their missions, these facilities are often remote and isolated from the utility grid. The preferred choice for power at these facilities has historically been manned diesel generators. The DoD Photovoltaic Review Committee, estimates that on the order of 350 million gallons of diesel fuel is burned each year to generate the 2000 GWh of electricity required to operate these remote military facilities. Other federal agencies, including the National Park Service and the USDA Forest Service use diesel generators for remote power needs as well. The generation of power diesel generators is both expensive and detrimental to the environment. The augmentation of power from diesel generators with power processing and battery energy storage enhances the efficiency and utilization of the generator resulting in lower fuel consumption and lower generator run- time in proportion to the amount of renewables added. The hybrid technology can both reduce the cost of power and reduce environmental degradation at remote DoD facilities. This paper describes the expected performance and economics of photovoltaic/diesel hybrid systems. Capabilities and status of systems now being installed at DoD facilities are presented along with financing mechanisms available within DoD.

  14. Licensing an assured isolation facility for low-level radioactive waste. Volume 2: Recommendations on the content and review of an application

    Energy Technology Data Exchange (ETDEWEB)

    Silverman, D.J.; Bauser, M.A. [Morgan, Lewis and Bockius, Washington, DC (United States); Baird, R.D. [Rogers and Associates Engineering Corp., Salt Lake City, UT (United States)

    1998-07-01

    This report provides a detailed set of proposed criteria and guidance for the preparation of a license application for an assured isolation facility (AIF). The report is intended to provide a detailed planning basis upon which a prospective applicant may begin pre-licensing discussions with the Nuclear Regulatory Commission and initiate development of a license application. The report may also be useful to the NRC or to state regulatory agencies that may be asked to review such an application. Volume 1 of this report provides background information, and describes the licensing approach and methodology. Volume 2 identifies specific information that is recommended for inclusion in a license application.

  15. Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors

    DEFF Research Database (Denmark)

    Ameri, Jacqueline; Borup, Rehannah; Prawiro, Christy

    2017-01-01

    cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2) as a specific cell surface marker for isolating pancreatic endoderm cells (PECs) from differentiated hESCs and human fetal pancreas. Isolated GP2+ PECs efficiently differentiated into glucose responsive insulin......-producing cells in vitro. We found that in vitro PEC proliferation declines due to enhanced expression of the cyclin-dependent kinase (CDK) inhibitors CDKN1A and CDKN2A. However, we identified a time window when reducing CDKN1A or CDKN2A expression increased proliferation and yield of GP2+ PECs. Altogether, our...... results contribute tools and concepts toward the isolation and use of PECs as a source for the safe production of hPSC-derived β cells....

  16. Third harmonic generation in isolated all dielectric meta-atoms (Conference Presentation)

    Science.gov (United States)

    Melik-Gaykazyan, Elizaveta V.; Shorokhov, Alexander S.; Shcherbakov, Maxim R.; Staude, Isabelle; Smirnova, Daria A.; Miroshnichenko, Andrey E.; Brener, Igal; Neshev, Dragomir N.; Fedyanin, Andrey A.; Kivshar, Yuri S.

    2016-04-01

    Two series of nanodisk arrays were designed. The first one was fabricated out of a silicon-on-insulator (SOI) wafer using electron-beam lithography and a reactive-ion etching process. The top layer of a SOI wafer is a 260-nm layer of monocrystalline (100)-cut silicon. We consider three square 400x400 μm2 arrays distinguished by the disk diameter values - 340, 345 and 360 nm, respectively; the period of the nanodisk ordering in the array amounted to 2.85 μm - this value allows for regarding the disks as isolated ones in terms of optical coupling. The nanodisk diameter choice specifies the magnetic dipolar (MD) resonance wavelength [1]. The second series of arrays was made of a 130-nm hydrogenated amorphous silicon (a-Si:H) film grown by plasma-enhanced chemical vapor deposition on a thin glass substrate. In order to study the nonlinear optical response of the nanodisks and verify the multipole resonances roles, we conducted third-harmonic generation (THG) spectroscopy measurements using a tunable (1.0-1.5 μm) optical parametric oscillator (200 fs pulses with the repetition rate of 76 MHz) pumped by a Ti:Sapphire laser. The laser beam waist diameter was set at 11 μm by an aspheric lens. The full thickness of both the SOI and glass wafers (˜500 μm each) was less than the waist depth. The resulting peak intensity reached the values of about 1 GW/cm2 in the sample plane. The laser beam polarization was linear as controlled by a Glan-Taylor laser prism. The transmitted and collimated THG signal was selected by a set of blue filters and detected by a photomultiplier tube connected with a lock-in amplifier. This signal was proven to be of TH origin by checking its cubic dependence on the pump power and by direct measurements of its spectrum. It was also verified that the THG beam was polarized parallel to the orientation of the pump beam polarization. It should be pointed out that the penetration depth of the THG into silicon does not exceed the nanodisk height. The

  17. Antibiotic resistance pattern of bacterial isolates from cases of urinary tract infections among hospitalized and out-patients at a tertiary health facility in South Western Nigeria

    Directory of Open Access Journals (Sweden)

    Oyekale Oluwalana Timothy

    2014-01-01

    Full Text Available Aim: Urinary tract infections (UTIs are among the most common human infections with distribution of causative agents and their susceptibility pattern to antibiotics varying from region to region. This study aimed at determining the bacterial uropathogens and their antibiotic resistance profile among patients in a Nigerian tertiary health care facility. Materials and Methods: Appropriate urine specimens (midstream/catheter specimen urine of all suspected cases of UTI by clinicians were processed in the medical microbiology laboratory for detection of significant bacteriuria. Bacteria uropathogens isolated were identified by standard biochemical tests and antibiotic susceptibility test to eight antibiotics was carried out on them using Kirby-Bauer disc diffusion technique. Methicillin-resistant Staphylococcus aureus (MRSA was identified by cefoxitin disc diffusion technique and extended-spectrum beta-lactamase (ESBL producing enterobacteria were detected using double-disc synergy test. Results: Of the total 157 males and 189 females investigated, 35.7% and 66.1% respectively had significant bacteriuria. Escherichia coli was the most commonly isolated bacterial pathogen both among in- and out-patients (52.6% vs. 65.5%. Other isolated organisms were S. aureus (13.4% vs. 19.0%, Pseudomonas aeruginosa (10.3% vs. 2.4%, Klebsiella pneumoniae (7.2% vs. 7.1% and K. aerogenes (7.2% vs. 1.2%. Resistance rate of uropathogens to antibiotics was higher among in-patients. Resistance rate to ofloxacin, ceftazidime and ceftriaxone was generally very low compared to other tested antibiotics. Multiple resistant bacteria: MRSA and ESBL-producing enterobacteria were detected among both in-and out-patient with no significant difference in isolation rate. Conclusion: There is a need for continuous monitoring of uropathogens and their antibiotic sensitivity profile for evidence-based empirical treatment of UTI. There is an urgent need for the establishment of antibiotic

  18. Generation of an isolated sub-30 attosecond pulse in a two-color laser field and a static electric field

    Institute of Scientific and Technical Information of China (English)

    Zhang Gang-Tai; Bai Ting-Ting; Zhang Mei-Guang

    2012-01-01

    We theoretically investigate high-order harmonic generation(HHG)from a helium ion model in a two-color laser field,which is synthesized by a fundamental pulse and its second harmonic pulse.It is shown that a supercontinuum spectrum can be generated in the two-color field.However,the spectral intensity is very low,limiting the application of the generated attosecond(as)pulse.By adding a static electric field to the synthesized two-color field,not only is the ionization yield of electrons contributing to the harmonic emission remarkably increased,but also the quantum paths of the HHG can be significantly modulated.As a result,the extension and enhancement of the supercontinuum spectrum are achieved,producing an intense isolated 26-as pulse with a bandwidth of about 170.5 eV.In particular,we also analyse the influence of the laser parameters on the ultrabroad supercontinuum spectrum and isolated sub-30-as pulse generation.

  19. Neural network-based voltage regulator for an isolated asynchronous generator supplying three-phase four-wire loads

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Bhim; Kasal, Gaurav Kumar [Department of Electrical Engineering, Indian Institute of Technology, Delhi, Hauz-Khas, New Delhi 110016 (India)

    2008-06-15

    This paper deals with a neural network-based solid state voltage controller for an isolated asynchronous generator (IAG) driven by constant speed prime mover like diesel engine, bio-gas or gasoline engine and supplying three-phase four-wire loads. The proposed control scheme uses an indirect current control and a fast adaptive linear element (adaline) based neural network reference current extractor, which extracts the real positive sequence current component without any phase shift. The neutral current of the source is also compensated by using three single-phase bridge configuration of IGBT (insulated gate bipolar junction transistor) based voltage source converter (VSC) along-with single-phase transformer having self-supported dc bus. The proposed controller provides the functions as a voltage regulator, a harmonic eliminator, a neutral current compensator, and a load balancer. The proposed isolated electrical system with its controller is modeled and simulated in MATLAB along with Simulink and PSB (Power System Block set) toolboxes. The simulated results are presented to demonstrate the capability of an isolated asynchronous generating system driven by a constant speed prime mover for feeding three-phase four-wire loads. (author)

  20. Methods to isolate a large amount of generative cells, sperm cells and vegetative nuclei from tomato pollen for omics analysis

    Directory of Open Access Journals (Sweden)

    Yunlong eLu

    2015-06-01

    Full Text Available The development of sperm cells from microspores involves a set of finely regulated molecular and cellular events and the coordination of these events. The mechanisms underlying these events and their interconnections remain a major challenge. Systems analysis of genome-wide molecular networks and functional modules with high-throughput omics approaches is crucial for understanding the mechanisms; however, this study is hindered because of the difficulty in isolating a large amount of cells of different types, especially generative cells (GCs, from the pollen. Here, we optimized the conditions of tomato pollen germination and pollen tube growth to allow for long-term growth of pollen tubes in vitro with sperm cells (SCs generated in the tube. Using this culture system, we developed methods for isolating GCs, SCs and vegetative-cell nuclei (VN from just-germinated tomato pollen grains and growing pollen tubes and their purification by Percoll density gradient centrifugation. The purity and viability of isolated GCs and SCs were confirmed by microscopy examination and fluorescein diacetate staining, respectively, and the integrity of VN was confirmed by propidium iodide staining. We could obtain about 1.5 million GCs and 2.0 million SCs each from 180 mg initiated pollen grains, and 10 million VN from 270 mg initiated pollen grains germinated in vitro in each experiment. These methods provide the necessary preconditions for systematic biology studies of SC development and differentiation in higher plants.

  1. Design of motor induction 3-Phase from waste industry to generator for microhydro at isolated village

    Science.gov (United States)

    Rimbawati; Azis Hutasuhut, Abdul; Irsan Pasaribu, Faisal; Cholish; Muharnif

    2017-09-01

    There is an electric machine that can operate as a generator either single-phase or three-phase in almost every household and industry today. This electric engine cannot be labeled as a generator but can be functioned as a generator. The machine that is mentioned is “squirrel cage motors” or it is well-known as induction motor that can be found in water pumps, washing machines, fans, blowers and other industrial machines. The induction motor can be functioned as a generator when the rotational speed of the rotor is made larger than the speed of the rotary field. In this regard, this study aims to modify the remains of 3-phase induction motor to be a permanent generator. Data of research based conducted on the river flow of Rumah Sumbul Village, STM Hulu district of Deli Serdang. The method of this research is by changing rotor and stator winding on a 3 phase induction motor, so it can produce a generator with rotation speed of 500 rpm. Based on the research, it can be concluded that the output voltage generator has occurred a voltage drop 10% between before and after loading for Star circuit and 2% for Delta circuit.

  2. HEDgeHOB High-energy density matter generated by heavy ion beams at the future facility for antiprotons and ion research

    CERN Document Server

    Tahir, N A; Shutov, A; Lomonosov, I V; Gryaznov, V; Piriz, A R; Wouchuk, G; Deutsch, C; Fortov, V E; Hoffmann, D H H; Schmidt, R

    2007-01-01

    This paper presents an overview of the theoretical work that has been carried out during the past few years to assess the capabilities of intense heavy ion beams to induce states of High-Energy Density (HED) in matter. This work has shown that two different experimental schemes can be used to study HED physics employing intense ion beams. These schemes have been named HIHEX [Heavy Ion Heating and EXpansion] and LAPLAS [LAboratory PLAnetary Sciences], respectively. The first scheme involves isochoric and uniform heating and subsequent isentropic expansion of matter while the latter deals with low entropy compression of matter using multiple shock reflection technique. This work has been done within the framework of the HEDgeHOB [High Energy Density Matter Generated by Heavy Ion Beams] collaboration that has been formed to organize and facilitate construction of experimental facilities and later to perform experimental work in the field of HED matter at the future accelerator facility, FAIR [Facility for Antipr...

  3. CrossRef Optimizing charge breeding techniques for ISOL facilities in Europe: Conclusions from the EMILIE project

    CERN Document Server

    Delahaye, P; Angot, J; Cam, J F; Traykov, E; Ban, G; Celona, L; Choinski, J; Gmaj, P; Jardin, P; Koivisto, H; Kolhinen, V; Lamy, T; Maunoury, L; Patti, G; Thuillier, T; Tarvainen, O; Vondrasek, R; Wenander, F

    2016-01-01

    The present paper summarizes the results obtained from the past few years in the framework of the Enhanced Multi-Ionization of short-Lived Isotopes for Eurisol (EMILIE) project. The EMILIE project aims at improving the charge breeding techniques with both Electron Cyclotron Resonance Ion Sources (ECRIS) and Electron Beam Ion Sources (EBISs) for European Radioactive Ion Beam (RIB) facilities. Within EMILIE, an original technique for debunching the beam from EBIS charge breeders is being developed, for making an optimal use of the capabilities of CW post-accelerators of the future facilities. Such a debunching technique should eventually resolve duty cycle and time structure issues which presently complicate the data-acquisition of experiments. The results of the first tests of this technique are reported here. In comparison with charge breeding with an EBIS, the ECRIS technique had lower performance in efficiency and attainable charge state for metallic ion beams and also suffered from issues related to beam c...

  4. Modeling of Self-Excited Isolated Permanent Magnet Induction Generator Using Iterative Numerical Method

    Directory of Open Access Journals (Sweden)

    Mohamed Mostafa R.

    2016-01-01

    Full Text Available Self-Excited Permanent Magnet Induction Generator (PMIG is commonly used in wind energy generation systems. The difficulty of Self-Excited Permanent Magnet Induction Generator (SEPMIG modeling is the circuit parameters of the generator vary at each load conditions due to the a change in the frequency and stator voltage. The paper introduces a new modeling for SEPMIG using Gauss-sidle relaxation method. The SEPMIG characteristics using the proposed method are studied at different load conditions according to the wind speed variation, load impedance changes and different shunted capacitor values. The system modeling is investigated due to the magnetizing current variation, the efficiency variation, the power variation and power factor variation. The proposed modeling system satisfies high degree of simplicity and accuracy.

  5. Efficient Generation of Glucose-Responsive Beta Cells from Isolated GP2+ Human Pancreatic Progenitors

    Directory of Open Access Journals (Sweden)

    Jacqueline Ameri

    2017-04-01

    Full Text Available Stem cell-based therapy for type 1 diabetes would benefit from implementation of a cell purification step at the pancreatic endoderm stage. This would increase the safety of the final cell product, allow the establishment of an intermediate-stage stem cell bank, and provide a means for upscaling β cell manufacturing. Comparative gene expression analysis revealed glycoprotein 2 (GP2 as a specific cell surface marker for isolating pancreatic endoderm cells (PECs from differentiated hESCs and human fetal pancreas. Isolated GP2+ PECs efficiently differentiated into glucose responsive insulin-producing cells in vitro. We found that in vitro PEC proliferation declines due to enhanced expression of the cyclin-dependent kinase (CDK inhibitors CDKN1A and CDKN2A. However, we identified a time window when reducing CDKN1A or CDKN2A expression increased proliferation and yield of GP2+ PECs. Altogether, our results contribute tools and concepts toward the isolation and use of PECs as a source for the safe production of hPSC-derived β cells.

  6. Operational generation of AVHRR-based cloud products for Europe and the Arctic at EUMETSAT's Satellite Application Facility on Climate Monitoring (CM-SAF

    Directory of Open Access Journals (Sweden)

    F. Kaspar

    2009-04-01

    Full Text Available The Satelite Application Facility on Climate Monitoring has implemented a new processing environment for AVHRR-based climate monitoring products. AVHRR measurements from NOAA-17, NOAA-18 and MetOp-A are utilized to generate daily and monthly means of several cloud parameters for Europe and the Inner Arctic: Cloud fraction, cloud types, cloud phase, cloud top height, cloud optical thickness and cloud liquid water path.

  7. Evaluation technology for burnup and generated amount of plutonium by measurement of xenon isotopic ratio in dissolver off-gas at reprocessing facility (Joint research)

    OpenAIRE

    岡野 正紀; 久野 剛彦; 高橋 一朗; 白水 秀知; Charlton, W. S.; Wells, C. A.; Hemberger, P. H.; 山田 敬二; 酒井 敏雄

    2006-01-01

    The amount of Pu in the spent fuel was evaluated from Xe isotopic ratio in off-gas in reprocessing facility, is related to burnup. Six batches of dissolver off-gas at spent fuel dissolution process were sampled from the main stack in Tokai Reprocessing Plant during BWR fuel reprocessing campaign. Xenon isotopic ratio was determined with GC/MS. Burnup and generated amount of Pu were evaluated with Noble Gas Environmental Monitoring Application code (NOVA), developed by Los Alamos National Labo...

  8. Isolated sub-10 attosecond pulse generation by a 6-fs driving pulse and a 5-fs subharmonic controlling pulse

    Directory of Open Access Journals (Sweden)

    Yunhui Wang

    2012-06-01

    Full Text Available We theoretically study high-order harmonic generation by quantum path control in a special two-color laser field, which is synthesized by a 6 fs/800 nm fundamental pulse and a weaker 5 fs/1600 nm subharmonic controlling pulse. Single quantum path is selected without optimizing any carrier phase, which not only broadens the harmonic bandwidth to 400 eV, but also enhances the harmonic conversion efficiency in comparison with the short-plus-long scheme, which is based on 5 fs/800 nm driving pulse and 6 fs/1600 nm control pulse. An isolated 8-attosecond pulse is produced with currently available ultrafast laser sources.

  9. Testable Subsystems Generation for Fault Detection and Isolation Using a Structural Matching Rank Algorithm Testability of an Electrical Circuit

    Directory of Open Access Journals (Sweden)

    Benazzouz Djamel

    2013-05-01

    Full Text Available In this study, an advanced way of dealing with testable subsystems and residual generation for fault detection and isolation based on structural analysis is presented. The developed technique considers execution issues; therefore, it has a more realistic point of view compared to classical structural approaches available in the literature. First, theoretical aspects of structural analysis are considered and introduced. Then the way of incorporating them to test the structural proprieties is explained. Finally, we show how the proposed (upgraded matching rank algorithm can be used in order to choose the most suited matching that leads to computational sequences and detection tests. The method is demonstrated using an electrical circuit.

  10. Novae from isolated white dwarfs as a source of helium for second generation stars in globular clusters

    CERN Document Server

    Maccarone, Thomas J

    2011-01-01

    We explore the possible contribution of classical and recurrent novae from isolated white dwarfs accreting from the intracluster medium to the abundances of "second generation" globular cluster stellar populations. We show that under reasonable assumptions the helium abundances of clusters can be enhanced substantially by these novae and argue that novae should be considered as an important, and perhaps even dominant channel in the evolution of the intracluster medium. We also discuss a possible test for whether helium enhancement really is the cause of the multiple main sequences in globular clusters that is independent of the positions of stars in the color-magnitude diagram.

  11. Waste Isolation Pilot Plant (WIPP) conceptual design report. Part I: executive summary. Part II: facilities and system

    Energy Technology Data Exchange (ETDEWEB)

    1977-06-01

    The pilot plant is developed for ERDA low-level contact-handled transuranic waste, ERDA remote-handled intermediate-level transuranic waste, and for high-level waste experiments. All wastes placed in the WIPP arrive at the site processed and packaged; no waste processing is done at the WIPP. All wastes placed into the WIPP are retrievable. The proposed site for WIPP lies 26 miles east of Carlsbad, New Mexico. This document includes the executive summary and a detailed description of the facilities and systems. (DLC)

  12. Integrated O&M for energy generation and exchange facilities; O&M integral para instalaciones de generación e intercambio de energía

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2016-07-01

    Ingeteam Service, part of the Ingeteam Group, is a leading company in the provision of integrated O&M services at energy generation and exchange facilities worldwide. From its head office in the Albacete Science and Technology Park, it manages the work of the 1,300 employees that make up its global workforce, rendering services to wind farms, PV installations and power generation plants. In addition, it maintains an active participation strategy in a range of R&D+i programmes that improve the existing technologies and are geared towards new production systems and new diagnostic techniques, applied to renewables installation maintenance. (Author)

  13. The ISOLDE Facility: Radioactive beams at CERN

    CERN Document Server

    CERN. Geneva

    2007-01-01

    The Isope Separation On-Line (ISOL) technique evolved from chemical techniques used to separate radioactive isotopes off-line from irradiated "targets". The ISOL targets of today, used at e.g. ISOLDE, can be of many different types and in different phases but the isotopes are always delivered at very low energies making the technique ideal for study of ground state properties and collections for other applications such as solid state physics and medical physics. The possibility of accelerating these low energy beams for nuclear structure studies, and in the long term future for neutrino physics, is now being explored at first generation radioactive beam facilities. The upgrade towards HIE-ISOLDE aim to consolidate ISOLDE's position as a world leading radioactive nuclear beam facility and it will be a pre-cursor to a future all European ISOL facility, EURISOL, with order of magnitudes higher radioactive beam intensities and energies. Prerequisite knowledge and references: None

  14. Assessment of effectiveness of geologic isolation systems. CIRMIS data system. Volume 3. Generator routines

    Energy Technology Data Exchange (ETDEWEB)

    Friedrichs, D.R.; Argo, R.S.

    1980-01-01

    The Assessment of Effectiveness of Geologic Isolation Systems (AEGIS) Program is developing and applying the methodology for assessing the far-field, long-term post-closure safety of deep geologic nuclear waste repositories. AEGIS is being performed by Pacific Northwest Laboratory (PNL) under contract with the Office of Nuclear Waste Isolation (ONWI) for the Department of Energy (DOE). One task within AEGIS is the development of methodology for analysis of the consequences (water pathway) from loss of repository containment as defined by various release scenarios. The various input parameters required in the analysis are compiled in data systems. The data are organized and prepared by various input subroutines for utilization by the hydraulic and transport codes. The hydrologic models simulate the groundwater flow systems and provide water flow directions, rates, and velocities as inputs to the transport models. Outputs from the transport models are basically graphs of radionuclide concentration in the groundwater plotted against time. After dilution in the receiving surface-water body (e.g., lake, river, bay), these data are the input source terms for the dose models, if dose assessments are required. The dose models calculate radiation dose to individuals and populations. CIRMIS (Comprehensive Information Retrieval and Model Input Sequence) Data System, a storage and retrieval system for model input and output data, including graphical interpretation and display is described. This is the third of four volumes of the description of the CIRMIS Data System.

  15. Isometric and eccentric force generation assessment of skeletal muscles isolated from murine models of muscular dystrophies.

    Science.gov (United States)

    Moorwood, Catherine; Liu, Min; Tian, Zuozhen; Barton, Elisabeth R

    2013-01-31

    Critical to the evaluation of potential therapeutics for muscular disease are sensitive and reproducible physiological assessments of muscle function. Because many pre-clinical trials rely on mouse models for these diseases, isolated muscle function has become one of the standards for Go/NoGo decisions in moving drug candidates forward into patients. We will demonstrate the preparation of the extensor digitorum longus (EDL) and diaphragm muscles for functional testing, which are the predominant muscles utilized for these studies. The EDL muscle geometry is ideal for isolated muscle preparations, with two easily accessible tendons, and a small size that can be supported by superfusion in a bath. The diaphragm exhibits profound progressive pathology in dystrophic animals, and can serve as a platform for evaluating many potential therapies countering fibrosis, and promoting myofiber stability. Protocols for routine testing, including isometric and eccentric contractions, will be shown. Isometric force provides assessment of strength, and eccentric contractions help to evaluate sarcolemma stability, which is disrupted in many types of muscular dystrophies. Comparisons of the expected results between muscles from wildtype and dystrophic muscles will also be provided. These measures can complement morphological and biochemical measurements of tissue homeostasis, as well as whole animal assessments of muscle function.

  16. Design of DC-DC Converter and its Control for a Wind Generation System Connected to an Isolated Load

    Directory of Open Access Journals (Sweden)

    Carlos A. Ramírez Gómez

    2013-11-01

    Full Text Available A method to design a Buck converter and its control, which are associated to a wind generation system that is feeding an isolated load, is presented in this paper. To design the converter a Thevenin equivalent is deduced, which represents the behavior of the wind turbine, the permanent magnet synchronous generator, and the rectifier. The design of the converter elements guarantees input/output voltages and inductor current ripples of 5 % or less. The output voltage control is developed with a proportional-integral-derivative controller and as design criteria a damping of 0,707 and cutoff frequency of 1/5 converter commutation frequency are selected. The designed controller regulates the output voltage faced load perturbations and wind speed variations. 

  17. Facile production of nanostructured cellulose from Elaeis guineensis empty fruit bunch via one pot oxidative-hydrolysis isolation approach.

    Science.gov (United States)

    Chen, You Wei; Lee, Hwei Voon; Abd Hamid, Sharifah Bee

    2017-02-10

    Cellulose in nanostructures was successfully isolated from empty fruit bunch biomass via a novel one-pot oxidative-hydrolysis technique. The physicochemical properties of nanocellulose prepared via one-pot process have shown comparable characteristics as products isolated via conventional multistep purification approach (namely dewaxing, chlorite bleaching process, alkalization, and acid hydrolysis). The chemical composition study indicated that the one-pot oxidative-hydrolysis process successfully extracted cellulose (91.0%), with the remaining minority being hemicellulose and lignin (∼6%) in the final product. Crystallinity profile of one-pot treated product (80.3%) was higher than that of multistep isolated nanocellulose (75.4%), which indicated that the disorder region (amorphous) in cellulose fibers was successfully removed. In additional to that, the morphology study demonstrated that nanocellulose prepared by one-pot process rendered spider-web-like network nanostructure, with an average diameter of fibers at a range of 51.6±15.4nm. The nanocellulose product showed high thermal stability (320°C), which was ready for nanocomposite application. One-pot oxidative-hydrolysis technique is a simple and versatile route for the preparation of nanocellulose from complex biomass within 90°C and 6h period, with minimum wastewater as compared to the multistep process.

  18. Identification of novel open reading frames from metagenomic libraries generated from extremophilic organisms: application of metagenomics and high throughput screening for novel enzyme isolation

    CSIR Research Space (South Africa)

    Visser, Daniel F

    2006-10-01

    Full Text Available South African mines. Genomic DNA was isolated from these biofilms, and various metagenomic libraries generated. These libraries were in turn screened for industrially important enzymes, in particular proteases and lipases. Resultant hits had plasmid DNA...

  19. Generation and isolation of target-specific single-domain antibodies from shark immune repertoires.

    Science.gov (United States)

    Müller, Mischa Roland; O'Dwyer, Ronan; Kovaleva, Marina; Rudkin, Fiona; Dooley, Helen; Barelle, Caroline Jane

    2012-01-01

    The drive to exploit novel targets and biological pathways has lead to the expansion of classical antibody research into innovative fragment adaptations and novel scaffolds. The hope being that alternative or cryptic epitopes may be targeted, tissue inaccessibility may be overcome, and easier engineering options will facilitate multivalent, multi-targeting approaches. To this end, we have been isolating shark single domains to gain a greater understanding of their potential as therapeutic agents. Their unique shape, small size, inherent stability, and simple molecular architecture make them attractive candidates from a drug discovery perspective. Here we describe protocols to capture the immune repertoire of an immunized shark species and to build and select via phage-display target-specific IgNAR variable domains (VNARs).

  20. Test Generation and Fault Isolation for Microprocessors and Their Support Devices.

    Science.gov (United States)

    1980-11-01

    ISOLATION •CURRENT »IISOL II 12 FORCEO VOLTAGE IMIN • ••EAS’JRE CONTI- • NUIT » ON INPUT «»ICON II 12 FORCEO CURRENT V«1N •-F•SuBf OUTPUT...0.0200 < 0.0300 < ’SPACE S- I.A-5-0-1 -«-3 •3-6,10,«3.5« 0.0400 • 0.0500 ’ 0.0600 < 0.0700 • 0.0*00 i sss pppppppp Mill GGIL c HMMMវ...registers in an array, as there were with the 2901 A, but there is a great deal more flexibility with their 138 Mill 1^*1 I J p ’ •’ " " mm

  1. Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.

    Science.gov (United States)

    Albariño, César G; Uebelhoer, Luke S; Vincent, Joel P; Khristova, Marina L; Chakrabarti, Ayan K; McElroy, Anita; Nichol, Stuart T; Towner, Jonathan S

    2013-11-01

    Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5' and 3' RACE, followed by the cloning of viral proteins NP, VP35, VP30 and L into expression plasmids. Novel conditions were then developed to efficiently replicate virus mini-genomes followed by the construction of full-length genomic clones from which recombinant wild type and GFP-containing MARVs were rescued. Surprisingly, when these recombinant MARVs were propagated in primary human macrophages, a dramatic difference was found in their ability to grow and to elicit anti-viral cytokine responses.

  2. Non-collinear Generation of Angularly Isolated Circularly Polarized High Harmonics

    Science.gov (United States)

    2015-09-21

    the electromagnetic field propagator49. The target (gas jet) was discretized into elementary radiators and propagated the emitted field Ej(rd,t) to...harmonic generation using analytical descriptions in both the photon and wave models. Advanced numerical simulations indicate that this non-collinear mixing...collinear HHG using both intuitive physical models as well as advanced numerical calculations. In the photon picture (Fig. 1b), we show that the NCP

  3. Electricity generation at high ionic strength in microbial fuel cell by a newly isolated Shewanella marisflavi EP1

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Jiexun [Univ. of Science and Technology of China, Hefei (China). Hefei National Lab. for Physical Sciences at Microscale and School of Life Sciences; State Oceanic Administration, Xiamen (China). Key Lab. of Marine Biogenetic Resources; Sun, Baolin [Univ. of Science and Technology of China, Hefei (China). Hefei National Lab. for Physical Sciences at Microscale and School of Life Sciences; Zhang, Xiaobo [Zhejiang Univ., Hangzhou (China). Coll. of Life Sciences; State Oceanic Administration, Xiamen (China). Key Lab. of Marine Biogenetic Resources

    2010-01-15

    Increasing the ionic strength of the electrolyte in a microbial fuel cell (MFC) can remarkably increase power output due to the reduction of internal resistance. However, only a few bacterial strains are capable of producing electricity at a very high ionic strength. In this report, we demonstrate a newly isolated strain EP1, belonging to Shewanella marisflavi based on polyphasic analysis, which could reduce Fe(III) and generate power at a high ionic strength of up to 1,488 mM (8% NaCl) using lactate as the electron donor. Using this bacterium, a measured maximum power density of 3.6 mW/m{sup 2} was achieved at an ionic strength of 291 mM. The maximum power density was increased by 167% to 9.6 mW/m{sup 2} when ionic strength was increased to 1,146 mM. However, further increasing the ionic strength to 1,488 mM resulted in a decrease in power density to 5.2 mW/m{sup 2}. Quantification of the internal resistance distribution revealed that electrolyte resistance was greatly reduced from 1,178 to 50 {omega} when ionic strength increased from 291 to 1,488 mM. These results indicate that isolation of specific bacterial strains can effectively improve power generation in some MFC applications. (orig.)

  4. A Healthy Investment: Building the Facilities to Train the Next Generation of Nursing and Allied Health Professionals

    Science.gov (United States)

    Woods, Bob

    2013-01-01

    A growing number of community colleges are investing in new facilities and programs to train health care workers in a variety of professions, including nursing, radiology, health information technology, physical therapy, dentistry, and surgical technology. Community colleges have historically offered job training programs in health care, but with…

  5. A Healthy Investment: Building the Facilities to Train the Next Generation of Nursing and Allied Health Professionals

    Science.gov (United States)

    Woods, Bob

    2013-01-01

    A growing number of community colleges are investing in new facilities and programs to train health care workers in a variety of professions, including nursing, radiology, health information technology, physical therapy, dentistry, and surgical technology. Community colleges have historically offered job training programs in health care, but with…

  6. Dual-species biofilm formation by Escherichia coli O157:H7 and environmental bacteria isolated from fresh-cut processing facilities.

    Science.gov (United States)

    Liu, Nancy T; Nou, Xiangwu; Lefcourt, Alan M; Shelton, Daniel R; Lo, Y Martin

    2014-02-03

    Biofilm formation is a mechanism adapted by many microorganisms that enhances the survival in stressful environments. In food processing facilities, foodborne bacterial pathogens, which many are poor biofilm formers, could potentially take advantage of this protective mechanism by interacting with other strong biofilm producers. The objective of this study was to determine the influence of bacteria native to fresh produce processing environments on the incorporation of Escherichia coli O157:H7 in biofilms. Bacteria strains representing 13 Gram-negative species isolated from two fresh produce processing facilities in a previous study were tested for forming dual-species biofilms with E. coli O157:H7. Strong biofilm producing strains of Burkholderia caryophylli and Ralstonia insidiosa exhibited 180% and 63% increase in biofilm biomass, and significant thickening of the biofilms (B. caryophylli not tested), when co-cultured with E. coli O157:H7. E. coli O157:H7 populations increased by approximately 1 log in dual-species biofilms formed with B. caryophylli or R. insidiosa. While only a subset of environmental isolates with strong biofilm formation abilities increased the presence of E. coli O157:H7 in biofilms, all tested E. coli O157:H7 exhibited higher incorporation in dual-species biofilms with R. insidiosa. These observations support the notion that E. coli O157:H7 and specific strong biofilm producing bacteria interact synergistically in biofilm formation, and suggest a route for increased survival potential of E. coli O157:H7 in fresh produce processing environments.

  7. Spread of CTX-M-type ESßLs in isolates of E. coli from long-term care and rehabilitation facilities in Northern Italy

    Directory of Open Access Journals (Sweden)

    Elisabetta Nucleo

    2008-09-01

    Full Text Available During the period March 2003 – May 2004 at the Laboratory of Clinical Microbiology “Redaelli” LTCRF in Milan, Italy, a total of 529 E. coli, obtained from inpatients of 3 different Long Term Care Rehabilitation Facilities (LTCRFs in Northern Italy, were processed and 77 ESßLs producers (14.5% were identified by Vitek System. The results were confirmed by double-disk synergy test with tazobactam (TZP. 61/77 isolates were characterized by higher levels of resistance to cefotaxime (CTX than to ceftazidime (CAZ. (ß-lactamase production was investigated by analytical isoelectric focusing (IEF coupled with a bioassay and showed multiple (ß-lactamase bands including one enzyme with pI 8.4 that, in a bioassay, was more active on CTX,ATM than on CAZ. The presence of (ß-lactamase genes was investigated by colony blot hybridization and by PCR amplification of blaTEM, blaSHV and blaCTX-M alleles. 43/61 isolates produced both TEM-1 and CTX-M-type enzymes, 14/61 expressed only CTX-M-type while in 4 cases were found blaCTX-M, blaTEM and blaSHV genes.The remainders (16/77, characterized by high levels of resistance to both CTX and CAZ, produced TEM-1 and SHV-5 enzymes (1/16 and TEM type ESßLs (15/16. Conjugation experiments, performed in liquid medium, confermed that the ESßLs determinants were transferable. Pulsed-field gel electrophoresis profiles of genomic DNA, digested with NotI, were analysed and revealed clonal heterogeneity. Our work confirms the emergence of CTX-M-type enzymes and their spread in Northern Italy also in longterm care and rehabilitation facilities that may be an important reservoir of ES?L producing E. coli.

  8. Computation of a Reference Model for Robust Fault Detection and Isolation Residual Generation

    Directory of Open Access Journals (Sweden)

    Emmanuel Mazars

    2008-01-01

    Full Text Available This paper considers matrix inequality procedures to address the robust fault detection and isolation (FDI problem for linear time-invariant systems subject to disturbances, faults, and polytopic or norm-bounded uncertainties. We propose a design procedure for an FDI filter that aims to minimize a weighted combination of the sensitivity of the residual signal to disturbances and modeling errors, and the deviation of the faults to residual dynamics from a fault to residual reference model, using the ℋ∞-norm as a measure. A key step in our procedure is the design of an optimal fault reference model. We show that the optimal design requires the solution of a quadratic matrix inequality (QMI optimization problem. Since the solution of the optimal problem is intractable, we propose a linearization technique to derive a numerically tractable suboptimal design procedure that requires the solution of a linear matrix inequality (LMI optimization. A jet engine example is employed to demonstrate the effectiveness of the proposed approach.

  9. Conformers, infrared spectrum, UV-induced photochemistry, and near-IR-induced generation of two rare conformers of matrix-isolated phenylglycine

    Science.gov (United States)

    Borba, Ana; Gómez-Zavaglia, Andrea; Fausto, Rui

    2014-10-01

    The conformational space of α-phenylglycine (PG) have been investigated theoretically at both the DFT/B3LYP/6-311++G(d,p) and MP2/6-311++G(d,p) levels of approximation. Seventeen different minima were found on the investigated potential energy surfaces, which are characterized by different dominant intramolecular interactions: type I conformers are stabilized by hydrogen bonds of the type N-H...O=C, type II by a strong O-H...N hydrogen bond, type III by weak N-H...O-H hydrogen bonds, and type IV by a C=O...H-C contact. The calculations indicate also that entropic effects are relevant in determining the equilibrium populations of the conformers of PG in the gas phase, in particular in the case of conformers of type II, where the strong intramolecular O-H...N hydrogen bond considerably diminishes entropy by reducing the conformational mobility of the molecule. In consonance with the relative energies of the conformers and barriers for conformational interconversion, only 3 conformers of PG were observed for the compound isolated in cryogenic Ar, Xe, and N2 matrices: the conformational ground state (ICa), and forms ICc and IITa. All other significantly populated conformers existing in the gas phase prior to deposition convert either to conformer ICa or to conformer ICc during matrix deposition. The experimental observation of ICc had never been achieved hitherto. Narrowband near-IR irradiation of the first overtone of νOH vibrational mode of ICa and ICc in nitrogen matrices (at 6910 and 6930 cm-1, respectively) led to selective generation of two additional conformers of high-energy, ITc and ITa, respectively, which were also observed experimentally for the first time. In addition, these experiments also provided the key information for the detailed vibrational characterization of the 3 conformers initially present in the matrices. On the other hand, UV irradiation (λ = 255 nm) of PG isolated in a xenon matrix revealed that PG undergoes facile photofragmentation

  10. In-phantom characterisation studies at the Birmingham Accelerator-Generated epIthermal Neutron Source (BAGINS) BNCT facility.

    Science.gov (United States)

    Culbertson, Christopher N; Green, Stuart; Mason, Anna J; Picton, David; Baugh, Gareth; Hugtenburg, Richard P; Yin, Zaizhe; Scott, Malcolm C; Nelson, John M

    2004-11-01

    A broad experimental campaign to validate the final epithermal neutron beam design for the BNCT facility constructed at the University of Birmingham concluded in November 2003. The final moderator and facility designs are overviewed briefly, followed by a summary of the dosimetric methods and presentation of a small subset of the results from this campaign. The dual ionisation chamber technique was used together with foil activation to quantify the fast neutron, photon, and thermal neutron beam dose components in a large rectangular phantom exposed to the beam with a 12 cm diameter beam delimiter in place. After application of a normalisation factor, dose measurements agree with in-phantom MCNP4C predictions within 10% for the photon dose, within 10% for thermal neutron dose, and within 25% for the proton recoil dose along the main beam axis.

  11. Evaluation of the efficacy of bleach routinely used in health facilities against Mycobacterium tuberculosis isolates in Ethiopia.

    Science.gov (United States)

    Mekonnen, Daniel; Admassu, Aschalew; Wassie, Belaynew; Biadglegne, Fantahun

    2015-01-01

    In Ethiopia, the most widely used disinfectant is 5% Hypochlorites. However, Ethiopian national health safety and infection prevention guideline recommendation on the use of bleach is not consistent and varying from 0.1%-4%. The purpose of this study was therefore to assess the effective time-concentration relationship of sodium hypochlorite against Mycobacterium tuberculosis complex isolates in the absence of any organic load. This experimental study was conducted in Bahir Dar Regional laboratory from February-June 2013. Test suspensions of 1.5 X 10(8) CFU/ml prepared using normal saline containing 0.5% tween 80. From 5% stock, 0.1%, 0.5%, 1% and 2% bleach was prepared. A 1ml of test strain suspension and 1ml of bleach mixed and allowed to stand until the specified time achieved, neutralized by 48 ml phosphate buffer. 100µl from the diluted sediment were spread on two L-J mediums and incubated at 37°C for 8 weeks. When 0. 1% bleach was used for 10 min, majority 11/20 of isolates showed 3 x 10(3) CFU/ml growth (ME = 4.4) which was inefficient. However, when the time increased, the log10 reduction was acceptable, ME >5 and it was effective. The bleach solution containing 0.5% and above was effective in all respective times. In this study, there is no difference observed in the tuberculocidal activity of bleach against resistant and sensitive strains. Our study showed that in the absence of any organic load, 0.1% bleaches over 15 min and 0.5% bleaches over 10 min was found to be tuberculocidal.

  12. Non-Genetic Engineering Approaches for Isolating and Generating Novel Yeasts for Industrial Applications

    Science.gov (United States)

    Chambers, P. J.; Bellon, J. R.; Schmidt, S. A.; Varela, C.; Pretorius, I. S.

    Generating novel yeast strains for industrial applications should be quite straightforward; after all, research into the genetics, biochemistry and physiology of Baker's Yeast, Saccharomyces cerevisiae, has paved the way for many advances in the modern biological sciences. We probably know more about this humble eukaryote than any other, and it is the most tractable of organisms for manipulation using modern genetic engineering approaches. In many countries, however, there are restrictions on the use of genetically-modified organisms (GMOs), particularly in foods and beverages, and the level of consumer acceptance of GMOs is, at best, variable. Thus, many researchers working with industrial yeasts use genetic engineering techniques primarily as research tools, and strain development continues to rely on non-GM technologies. This chapter explores the non-GM tools and strategies available to such researchers.

  13. Experimental investigation for an isolation technique on conducting the electromechanical impedance method in high-temperature pipeline facilities

    Science.gov (United States)

    Na, Wongi S.; Lee, Hyeonseok

    2016-11-01

    In general, the pipelines within a nuclear power plant facility may experience high temperatures up to several hundred degrees. Thus it is absolutely vital to monitor these pipes to prevent leakage of radioactive substances which may lead to a catastrophic outcome of the surrounding environment. Over the years, one of the structural health monitoring technique known as the electromechanical impedance (EMI) technique has been of great interests in various fields including civil infrastructures, mechanical and aerospace structures. Although it has one of the best advantages to be able for a single piezoelectric transducer to act as a sensor and an actuator, simultaneously, its low curie temperature makes it difficult for the EMI technique to be conducted at high temperature environment. To overcome this problem, this study shows a method to avoid attaching the piezoelectric transducer directly onto the target structure using a metal wire for damage detection at high temperature. By shifting the frequency to compensate the signature changes subjected to the variations in temperature, the experimental results indicate that damage identification is more successful above 200 oC, making the metal wire method suitable for the EMI technique at high temperature environment.

  14. Spectrally-isolated violet to blue wavelength generation by cascaded degenerate four-wave mixing in a photonic crystal fiber.

    Science.gov (United States)

    Yuan, Jinhui; Kang, Zhe; Li, Feng; Zhang, Xianting; Zhou, Guiyao; Sang, Xinzhu; Wu, Qiang; Yan, Binbin; Zhou, Xian; Wang, Liang; Zhong, Kangping; Wang, Kuiru; Yu, Chongxiu; Tam, Hwa Yaw; Wai, P K A

    2016-06-01

    Generation of spectrally-isolated wavelengths in the violet to blue region based on cascaded degenerate four-wave mixing (FWM) is experimentally demonstrated for the first time in a tailor-made photonic crystal fiber, which has two adjacent zero dispersion wavelengths (ZDWs) at 696 and 852 nm in the fundamental mode. The influences of the wavelength λp and the input average power Pav of the femtosecond pump pulses on the phase-matched frequency conversion process are studied. When femtosecond pump pulses at λp of 880, 870, and 860 nm and Pav of 500 mW are coupled into the normal dispersion region close to the second ZDW, the first anti-Stokes waves generated near the first ZDW act as a secondary pump for the next FWM process. The conversion efficiency ηas2 of the second anti-Stokes waves, which are generated at the violet to blue wavelengths of 430, 456, and 472 nm, are 4.8, 6.48, and 9.66%, for λp equalling 880, 870, and 860 nm, respectively.

  15. Electricity generation cost in isolated system: The complementarities of natural gas and renewables in the Canary Islands

    Energy Technology Data Exchange (ETDEWEB)

    Marrero, Gustavo A. [Facultad de Ciencias Economicas, Campus de Guajara, Universidad de La Laguna, 38071 Santa Cruz de Tenerife (Canary Islands) (Spain); Fundacion de Estudios de Economia Aplicada (FEDEA), C/Jorge Juan 46, 28001 Madrid (Spain); Ramos-Real, Francisco Javier [Facultad de Ciencias Economicas, Campus de Guajara, Universidad de La Laguna, 38071 Santa Cruz de Tenerife (Canary Islands) (Spain); Instituto Universitario de Desarrollo Regional, Facultad de Ciencias Economicas, Campus de Guajara, Universidad de La Laguna, 38071 Santa Cruz de Tenerife (Canary Islands) (Spain)

    2010-12-15

    The Canary Islands offer an example of an isolated electric grid of relative important size within the EU. Due to its peculiarities, the role of renewable energies and their complementarity with fossil fuels offers a solid path to achieving the main energy policy goals of the Islands. The purpose of this paper is to assess the current situation and the energy objectives proposed in the Energy Plan of the Canaries (PECAN, 2006) for the electricity industry, taking into account the average cost and the risk associated with the different alternatives for generating electricity by means of the Mean-Variance Portfolio Theory. Our analysis highlights the inefficiency of the current electricity generating mix in terms of cost, risk and lack of diversification. Shifting toward an efficient system would involve optimizing the use of endogenous energy sources and introducing natural gas to generate electricity. This scenario would mean reducing both cost and risk by almost 30% each, as well as atmospheric CO{sub 2} emissions. Our results agree with the PECAN philosophy. (author)

  16. Using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities.

    Science.gov (United States)

    Jałowiecki, Łukasz; Chojniak, Joanna; Dorgeloh, Elmar; Hegedusova, Berta; Ejhed, Helene; Magnér, Jörgen; Płaza, Grażyna

    2017-04-27

    The scope of the study was to apply Phenotype Biolog MicroArray (PM) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. In the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. In the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria were 53, 56, and 42%, respectively. Meanwhile, the shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria in the treated wastewater were 39, 33, and 29%, respectively. To evaluate the antibiotic susceptibility of the bacteria present in the wastewater, using the phenotype microarrays (PMs), the most common isolates from the treated wastewater were chosen: Serratia marcescens ss marcescens, Pseudomonas fluorescens, Stenotrophomonas maltophilia, Stenotrophomonas rhizophila, Microbacterium flavescens, Alcaligenes faecalis ss faecalis, Flavobacterium hydatis, Variovorax paradoxus, Acinetobacter johnsonii, and Aeromonas bestiarum. The strains were classified as multi-antibiotic-resistant bacteria. Most of them were resistant to more than 30 antibiotics from various chemical classes. Phenotype microarrays could be successfully used as an additional tool for evaluation of the multi-antibiotic resistance of environmental bacteria and in preliminary determination of the range of inhibition concentration.

  17. Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules.

    Science.gov (United States)

    Kiba, A; Miyake, C; Toyoda, K; Ichinose, Y; Yamada, T; Shiraishi, T

    1997-08-01

    ABSTRACT Fractions solubilized with NaCl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. Because superoxide dismutase decreased formazan production by over 90%, superoxide anion (O(2) ) may participate in the formation of formazan in the solubilized cell wall fractions. The formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of NAD(P)H, manganese ion, or p-coumaric acid from the reaction mixture. The formazan formation was severely inhibited by salicylhydroxamic acid and catalase, but not by imidazole, pyridine, quinacrine, and diphenyleneiodonium. An elicitor preparation from the pea pathogen Mycosphaerella pinodes enhanced the activities of formazan formation nonspecifically in both pea and cowpea fractions. The suppressor preparation from M. pinodes inhibited the activity in the pea fraction in the presence or absence of the elicitor. In the cowpea fraction, however, the suppressor did not inhibit the elicitor-enhanced activity, and the suppressor alone stimulated formazan formation. These results indicated that O(2) generation in the fractions solubilized from pea and cowpea cell walls seems to be catalyzed by cell wall-bound peroxidase(s) and that the plant cell walls alone are able to respond to the elicitor non-specifically and to the suppressor in a species-specific manner, suggesting the plant cell walls may play an important role in determination of plant-fungal pathogen specificity.

  18. Femtosecond electron pulse generation and measurement for diffractive imaging of isolated molecules

    Science.gov (United States)

    Zandi, Omid; Wilkin, Kyle J.; DeSimone, Alice J.; Yang, Jie; Centurion, Martin

    2016-09-01

    We have constructed an electron gun that delivers highly charged femtosecond electron pulses to a target with kHz repetition rate. Electron pulses are generated by femtosecond laser pulses in a photoemission process and are accelerated up to 100 kV and compressed to sub-picosecond duration. Compression is essential to compensate for the space charge effect that increases the size of electron pulses in all directions significantly. The pulses are compressed transversely by magnetic lenses and longitudinally by the longitudinal electric field of a radio-frequency cavity. The longitudinal compression is achieved by decelerating the electrons in the leading edge of the pulse, and accelerating the electrons in the trailing edge of the pulse. This results in the pulse compressing and reaching the minimum pulse duration at a known distance from the compression cavity. The short pulse duration and high repetition rate will be essential to observe subpicosecond dynamic processes in molecules in gas phase with a good signal to noise ratio. A streak camera, consisting of a millimeter-sized parallel plate capacitor, was used to measure the pulse duration in situ.

  19. The Effect Of Temperature On The Development Of Adult Generations Of Entomopathogenic Nematode Steinernema Arenarium Isolate CH

    Directory of Open Access Journals (Sweden)

    Yakovlev Ye. B.

    2015-08-01

    Full Text Available Steinernema arenarium isolate CH was prepared at 22 °C and used as a control in laboratory experiments on rearing in Galleria mellonella larvae at different temperatures (18 and 28 °C. Host dead bodies were examined every two days. All reared adult nematodes were fixed in alcohol and mounted on permanent slides with glycerin solution in distilled water. The basic morphometric parameters (L, W, ES, ABD (CBD, T, V were measured, and statistical analysis was performed. Morphometric data in males and females of both generations were shown to significantly change depending on speed of growth and nutrients concentration. In both experimental groups, pygmy forms of adults were found.

  20. Integrating ventilation monitoring sensor data with ventilation computer simulation software at the Waste Isolation Pilot Plant facility

    Energy Technology Data Exchange (ETDEWEB)

    Ruckman, R.; Prosser, B. [Mine Ventilation Services Inc., Clovis, CA (United States)

    2010-07-01

    This paper described an on-going ventilation study at an underground nuclear waste repository located in a bedded salt deposit in New Mexico. Underground airflow, differential pressure, primary fan information, and psychometric monitors were integrated into a ventilation model for the Waste Isolation Pilot Plant (WIPP). The WIPPVENT ventilation software is based on the commercially available package VnetPC developed by Mine Ventilation Services Inc. The ventilation system at WIPP has been tested and balanced since 1988. The work has involved re-engineering some of the ventilation system components in order to mitigate the effects of natural ventilation pressures. Ventilation monitoring system were also installed and remote control of the main underground regulators was achieved. Modifications were also made to the VnetPC ventilation software package to allow for continuous real-time updated ventilation models from the field measurement stations. This paper described the modifications to incorporate the real-time sensor data into the WIPPVENT program. 6 refs., 7 figs.

  1. Use of next generation sequence to investigate potential novel macrolide resistance mechanisms in a population of Moraxella catarrhalis isolates

    Science.gov (United States)

    Liu, Ya-Li; Li, Dong-Fang; Xu, He-Ping; Xiao, Meng; Cheng, Jing-Wei; Zhang, Li; Xu, Zhi-Peng; Chen, Xin-Xin; Zhang, Ge; Kudinha, Timothy; Kong, Fanrong; Gong, Yan-Ping; Wang, Xin-Ying; Zhang, Yin-Xin; Wu, Hong-Long; Xu, Ying-Chun

    2016-01-01

    Although previous studies have confirmed that 23S rRNA gene mutation could be responsible for most of macrolide resistance in M. catarrhalis, a recent study suggested otherwise. Next generation sequence based comparative genomics has revolutionized the mining of potential novel drug resistant mechanisms. In this study, two pairs of resistant and susceptible M. catarrhalis isolates with different multilocus sequence types, were investigated for potential differential genes or informative single nucleotide polymorphisms (SNPs). The identified genes and SNPs were evaluated in 188 clinical isolates. From initially 12 selected differential genes and 12 informative SNPs, 10 differential genes (mboIA, mcbC, mcbI, mboIB, MCR_1794, MCR_1795, lgt2B/C, dpnI, mcbB, and mcbA) and 6 SNPs (C619T of rumA, T140C of rplF, G643A of MCR_0020, T270G of MCR_1465, C1348A of copB, and G238A of rrmA) were identified as possibly linked to macrolide resistance in M. catarrhalis. Most of the identified differential genes and SNPs are related to methylation of ribosomal RNA (rRNA) or DNA, especially MCR_0020 and rrmA. Further studies are needed to determine the function and/or evolution process, of the identified genes or SNPs, to establish whether some novel or combined mechanisms are truly involved in M. catarrhalis macrolide resistance mechanism. PMID:27774989

  2. Improvements of the Computerized Data Acquisition System for 25MWt Experimental Facility of Coal-fired MHD Generator

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This paper introduces the design and development of a new computerized data acquisition system for the coal-fired magnetohydrodynamical (MHD) electrical power generation experiments. Compared to the previous system, it has a higher sampling rate and an improved simultaneity performance. It also improves the data collection method and sensor design for the measurement of Faraday voltages and Faraday currents. The system has been successfully used in many regular MHD generator tests. It provides an excellent base for the future research and development of the Coal-fired MHD electrical power generation.

  3. Solar powered hydrogen generating facility and hydrogen powered vehicle fleet. Final technical report, August 11, 1994--January 6, 1997

    Energy Technology Data Exchange (ETDEWEB)

    Provenzano, J.J.

    1997-04-01

    This final report describes activities carried out in support of a demonstration of a hydrogen powered vehicle fleet and construction of a solar powered hydrogen generation system. The hydrogen generation system was permitted for construction, constructed, and permitted for operation. It is not connected to the utility grid, either for electrolytic generation of hydrogen or for compression of the gas. Operation results from ideal and cloudy days are presented. The report also describes the achievement of licensing permits for their hydrogen powered trucks in California, safety assessments of the trucks, performance data, and information on emissions measurements which demonstrate performance better than the Ultra-Low Emission Vehicle levels.

  4. Generations.

    Science.gov (United States)

    Chambers, David W

    2005-01-01

    Groups naturally promote their strengths and prefer values and rules that give them an identity and an advantage. This shows up as generational tensions across cohorts who share common experiences, including common elders. Dramatic cultural events in America since 1925 can help create an understanding of the differing value structures of the Silents, the Boomers, Gen Xers, and the Millennials. Differences in how these generations see motivation and values, fundamental reality, relations with others, and work are presented, as are some applications of these differences to the dental profession.

  5. Magnetics Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Magnetics Research Facility houses three Helmholtz coils that generate magnetic fields in three perpendicular directions to balance the earth's magnetic field....

  6. The Potential Application of Gasification for Biomass Power Generation in Isolated Area from National Electricity Company in Indonesia

    Directory of Open Access Journals (Sweden)

    Kiman Siregar

    2016-01-01

    Full Text Available Indonesia has renewable energy source very large, as biomass. Installed capacity power plants of Indonesia now 53,585 MW, where the potential energy biomass is the largest of renewable sources of energy that owned by Indonesia. The machine of gasification is designed to produce combustible gas (CO, H2, CH4. These combustible gas are produced to replace fossil fuel as engine fuel to generate electricity. However, the properties and characteristics of these combustible gas should meet the characteristic of the engine as every engine has its own specific characteristic. In this research, Biomass Power Generation (BPG which work more properly with CO was used. High level of tar contained in produced combustible gas contaminates the engine filter rapidly. Therefore, the machine cannot be operated at a longer time. The objective of this research was to study the potential application of downdraft gasification machine with high content of CO and low production of tar. Downdraft gasification machine which designed in this research had installed capacity as much as 50 kW with reactor diameter and height were 900 mm and 1000 mm, respectively. The testing result showed that power generated from this machine was 40 kW, efficiency 80 % and can be operated for 6 hours. The optimum combustible gas produced by this machine occurred at CO, H2 and CH4 content were 21.75 %, 13.12 % and 1.12 %, respectively. Besides of using active carbon, tar removal was carried out using dust trap system (wet scrubber. The potential reduction of value of greenhouse gases emission is about 37 until 67 %. Based on an analysis calculation of financial , a test performance of gasification, and analysis of environmental impact, the use of system gasification can be used on a system a power plant in the isolated area from national electricity company in Indonesia.

  7. Idaho Waste Vitrification Facilities Project Vitrified Waste Interim Storage Facility

    Energy Technology Data Exchange (ETDEWEB)

    Bonnema, Bruce Edward

    2001-09-01

    This feasibility study report presents a draft design of the Vitrified Waste Interim Storage Facility (VWISF), which is one of three subprojects of the Idaho Waste Vitrification Facilities (IWVF) project. The primary goal of the IWVF project is to design and construct a treatment process system that will vitrify the sodium-bearing waste (SBW) to a final waste form. The project will consist of three subprojects that include the Waste Collection Tanks Facility, the Waste Vitrification Facility (WVF), and the VWISF. The Waste Collection Tanks Facility will provide for waste collection, feed mixing, and surge storage for SBW and newly generated liquid waste from ongoing operations at the Idaho Nuclear Technology and Engineering Center. The WVF will contain the vitrification process that will mix the waste with glass-forming chemicals or frit and turn the waste into glass. The VWISF will provide a shielded storage facility for the glass until the waste can be disposed at either the Waste Isolation Pilot Plant as mixed transuranic waste or at the future national geological repository as high-level waste glass, pending the outcome of a Waste Incidental to Reprocessing determination, which is currently in progress. A secondary goal is to provide a facility that can be easily modified later to accommodate storage of the vitrified high-level waste calcine. The objective of this study was to determine the feasibility of the VWISF, which would be constructed in compliance with applicable federal, state, and local laws. This project supports the Department of Energy’s Environmental Management missions of safely storing and treating radioactive wastes as well as meeting Federal Facility Compliance commitments made to the State of Idaho.

  8. Complete genome sequence and phenotype microarray analysis of Cronobacter sakazakii SP291: a persistent isolate cultured from a powdered infant formula production facility

    Directory of Open Access Journals (Sweden)

    Qiongqiong eYan

    2013-09-01

    Full Text Available Outbreaks of human infection linked to the powdered infant formula (PIF food chain and associated with the bacterium Cronobacter, are of concern to public health. These bacteria are regarded as opportunistic pathogens linked to life-threatening infections predominantly in neonates, with an under developed immune system. Monitoring the microbiological ecology of PIF production sites is an important step in attempting to limit the risk of contamination in the finished food product. Cronobacter species, like other microorganisms can adapt to the production environment. These organisms are known for their desiccation tolerance, a phenotype that can aid their survival in the production site and PIF itself. In evaluating the genome data currently available for Cronobacter species, no sequence information has been published describing a Cronobacter sakazakii isolate found to persist in a PIF production facility. Here we report on the complete genome sequence of one such isolate, Cronobacter sakazakii SP291 along with its phenotypic characteristics. The genome of C. sakazakii SP291 consists of a 4.3-Mb chromosome (56.9% GC and three plasmids, denoted as pSP291-1, [118.1-kb (57.2% GC], pSP291-2, [52.1-kb (49.2% GC] and pSP291-3, [4.4 -kb (54.0% GC]. When C. sakazakii SP291 was compared to the reference C. sakazakii ATCC BAA-894, which is also of PIF origin, the annotated genome data identified two interesting functional categories, comprising of genes related to the bacterial stress response and resistance to antimicrobial and toxic compounds. Using a phenotypic microarray (PM, we provided a full metabolic profile comparing C. sakazakii SP291 and the previously sequenced C. sakazakii ATCC BAA-894. These data extend our understanding of the genome of this important neonatal pathogen and provides further insights into the genotypes associated with features that can contribute to its persistence in the PIF environment.

  9. Complete genome sequence and phenotype microarray analysis of Cronobacter sakazakii SP291: a persistent isolate cultured from a powdered infant formula production facility.

    Science.gov (United States)

    Yan, Qiongqiong; Power, Karen A; Cooney, Shane; Fox, Edward; Gopinath, Gopal R; Grim, Christopher J; Tall, Ben D; McCusker, Matthew P; Fanning, Séamus

    2013-01-01

    Outbreaks of human infection linked to the powdered infant formula (PIF) food chain and associated with the bacterium Cronobacter, are of concern to public health. These bacteria are regarded as opportunistic pathogens linked to life-threatening infections predominantly in neonates, with an under developed immune system. Monitoring the microbiological ecology of PIF production sites is an important step in attempting to limit the risk of contamination in the finished food product. Cronobacter species, like other microorganisms can adapt to the production environment. These organisms are known for their desiccation tolerance, a phenotype that can aid their survival in the production site and PIF itself. In evaluating the genome data currently available for Cronobacter species, no sequence information has been published describing a Cronobacter sakazakii isolate found to persist in a PIF production facility. Here we report on the complete genome sequence of one such isolate, Cronobacter sakazakii SP291 along with its phenotypic characteristics. The genome of C. sakazakii SP291 consists of a 4.3-Mb chromosome (56.9% GC) and three plasmids, denoted as pSP291-1, [118.1-kb (57.2% GC)], pSP291-2, [52.1-kb (49.2% GC)], and pSP291-3, [4.4-kb (54.0% GC)]. When C. sakazakii SP291 was compared to the reference C. sakazakii ATCC BAA-894, which is also of PIF origin, the annotated genome data identified two interesting functional categories, comprising of genes related to the bacterial stress response and resistance to antimicrobial and toxic compounds. Using a phenotypic microarray (PM), we provided a full metabolic profile comparing C. sakazakii SP291 and the previously sequenced C. sakazakii ATCC BAA-894. These data extend our understanding of the genome of this important neonatal pathogen and provides further insights into the genotypes associated with features that can contribute to its persistence in the PIF environment.

  10. Thermal Analysis of Next Generation Space Telescope (NGST) Mirrors During Optical Testing in the X-Ray Calibration Facility (XRCF)

    Science.gov (United States)

    Page, Tim; Sutherlin, Steven; Hunt, Patrick L. (Technical Monitor)

    2001-01-01

    The Next Generation Space Telescope (NGST) program and industry partners are developing extremely lightweight mirror designs. NGST development mirrors are being tested at Marshall Space Flight Center. Target temperature for development mirror testing is 35 K. Conduction and radiation are not sufficient to conduct thermal vacuum testing in a reasonable time. Helium gas is injected into the vacuum chamber to accelerate temperature transitions during testing. Free-molecular conduction can be modeled by adapting present thermal analysis techniques.

  11. Studies of {beta}-delayed proton decays of N{approx_equal}Z nuclei around {sup 100}Sn at the GSI-ISOL facility

    Energy Technology Data Exchange (ETDEWEB)

    Mukha, I. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Batist, L. [St. Petersburg Nuclear Physics Institute, Gatchina (Russian Federation); Universita ' Federico II' and INFN Napoli, Naples (Italy); Becker, F. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Blazhev, A. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); University of Sofia, Sofia (Bulgaria); Bruechle, A. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Doering, J. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Gorska, M. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Grawe, H. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Faestermann, T. [Technical University, Munich (Germany); Hoffman, C. [Florida State University, Tallahassee, FL (United States); Janas, Z. [Warsaw University, Warsaw (Poland); Jungclaus, A. [Universidad Autonoma de Madrid (Spain); Karny, M. [Warsaw University, Warsaw (Poland); Kavatsyuk, M. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); National T. Shevchenko University, Kiev (Ukraine); Kavatsyuk, O. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Kirchner, R. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); La Commara, M. [Universita ' Federico II' and INFN Napoli, Naples (Italy); Mazzocchi, C. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Plettner, C. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Plochocki, A. [Warsaw University, Warsaw (Poland); Roeckl, E. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Romoli, M. [Universita ' Federico II' and INFN Napoli, Naples (Italy); Schaedel, M. [Gesellschaft fuer Schwerionenforschung, Darmstadt (Germany); Schwengner, R. [Inst. fuer Kern und Hadronenphysik, Forschungzentrum Rossendorf, Dresden (Germany)] [and others

    2004-12-27

    Beta decays of {sup 94,96}Ag and {sup 103}Sn nuclei into proton channels have been studied in the recent experiments at the GSI-ISOL facility. New efficient and chemically selective ion sources provided the highest yields of light silver and tin isotopes. Large arrays of germanium {gamma}-ray and silicon charged-particle detectors, as well as a total absorption spectrometer (TAS) were used to measure {beta}-proton, proton-{gamma}, {beta}-proton-{gamma} and proton-{gamma}-{gamma} spectra. For the decay of {sup 94}Ag, we observed high-spin states in {sup 93}Rh populated by proton emission following {beta} decay, whose largest spin value ({>=}39/2) yields an experimental proof for the existence of a second high-spin isomer in {sup 94}Ag with I{>=}17. Its {beta}-decay energy is at least 16.8 MeV, corresponding to an excitation energy {>=}5.5 MeV. For {sup 103}Sn, the {gamma} rays measured in coincidence with {beta}-delayed protons allowed us to establish the {beta}-decay properties of this isotope. In particular, a Q{sup EC} value of 7.5(6) MeV is derived from the intensity ratio of protons that are preceded either by EC or by {beta}{sup +} decays and populate the 2{sup +} state in {sup 102}Cd.

  12. Assessment of the Forward Contamination Risk of Mars by Clean Room Isolates from Space-Craft Assembly Facilities through Aeolian Transport - a Model Study

    Science.gov (United States)

    van Heereveld, Luc; Merrison, Jonathan; Nørnberg, Per; Finster, Kai

    2016-07-01

    The increasing number of missions to Mars also increases the risk of forward contamination. Consequently there is a need for effective protocols to ensure efficient protection of the Martian environment against terrestrial microbiota. Despite the fact of constructing sophisticated clean rooms for spacecraft assembly a 100 % avoidance of contamination appears to be impossible. Recent surveys of these facilities have identified a significant number of microbes belonging to a variety of taxonomic groups that survive the harsh conditions of clean rooms. These microbes may have a strong contamination potential, which needs to be investigate to apply efficient decontamination treatments. In this study we propose a series of tests to evaluate the potential of clean room contaminants to survive the different steps involved in forward contamination. We used Staphylococcus xylosus as model organism to illustrate the different types of stress that potential contaminants will be subjected to on their way from the spacecraft onto the surface of Mars. Staphylococcus xylosus is associated with human skin and commonly found in clean rooms and could therefore contaminate the spacecraft as a result of human activity during the assembling process. The path the cell will take from the surface of the spacecraft onto the surface of Mars was split into steps representing different stresses that include desiccation, freezing, aeolian transport in a Martian-like atmosphere at Martian atmospheric pressure, and UV radiation climate. We assessed the surviving fraction of the cellular population after each step by determining the integrated metabolic activity of the survivor population by measuring their oxygen consumption rate. The largest fraction of the starting culture (around 70 %) was killed during desiccation, while freezing, Martian vacuum and short-term UV radiation only had a minor additional effect on the survivability of Staphylococcus xylosus. The study also included a simulation

  13. Experimental Evaluation of Performance of Constant Power Prime-Mover Driven Isolated 3-φ SEIG for Pico-Hydro Power Generation System in Remote Mountainous Region of Himalayas

    Directory of Open Access Journals (Sweden)

    Rathore Umesh C.

    2016-01-01

    Full Text Available This paper presents the experimental evaluation of the performance of 3-φ self-excited induction generator (SEIG suitable for pico-hydro power generation system feeding domestic load in remote mountainous region. The use of induction generators is most suitable for renewable energy conversion systems due to their enormous advantages over conventional synchronous generators. Important features of induction generators include the simplicity in construction, ruggedness, simplified control, ease in maintenance and small size per generated kW. The performance characteristics of 3-φ SEIG feeding isolated load are evaluated using MATLAB-Simulink model based on the prevalent renewable energy sources inputs and loading conditions in mountainous terrain of Himalayas. The results are validated using an experimental set-up comprising of 3-φ, 3 HP induction motor run as 3- φ induction generator driven by 5HP, 4-pole DC shunt motor acting as prime-mover.

  14. High-energy-density plasmas generation on GEKKO-LFEX laser facility for fast-ignition laser fusion studies and laboratory astrophysics

    Science.gov (United States)

    Fujioka, S.; Zhang, Z.; Yamamoto, N.; Ohira, S.; Fujii, Y.; Ishihara, K.; Johzaki, T.; Sunahara, A.; Arikawa, Y.; Shigemori, K.; Hironaka, Y.; Sakawa, Y.; Nakata, Y.; Kawanaka, J.; Nagatomo, H.; Shiraga, H.; Miyanaga, N.; Norimatsu, T.; Nishimura, H.; Azechi, H.

    2012-12-01

    The world's largest peta watt (PW) laser LFEX, which delivers energy up to 2 kJ in a 1.5 ps pulse, has been constructed beside the GEKKO XII laser at the Institute of Laser Engineering, Osaka University. The GEKKO-LFEX laser facility enables the creation of materials having high-energy-density which do not exist naturally on the Earth and have an energy density comparable to that of stars. High-energy-density plasma is a source of safe, secure, environmentally sustainable fusion energy. Direct-drive fast-ignition laser fusion has been intensively studied at this facility under the auspices of the Fast Ignition Realization Experiment (FIREX) project. In this paper, we describe improvement of the LFEX laser and investigations of advanced target design to increase the energy coupling efficiency of the fast-ignition scheme. The pedestal of the LFEX pulse, which produces a long preformed plasma and results in the generation of electrons too energetic to heat the fuel core, was reduced by introducing an amplified optical parametric fluorescence quencher and saturable absorbers in the front-end system of the LFEX laser. Since fast electrons are scattered and stopped by the strong electric field of highly ionized high-Z (i.e. gold) ions, a low-Z cone was studied for reducing the energy loss of fast electrons in the cone tip region. A diamond-like carbon cone was fabricated for the fast-ignition experiment. An external magnetic field, which is demonstrated to be generated by a laser-driven capacitor-coil target, will be applied to the compression of the fuel capsule to form a strong magnetic field to guide the fast electrons to the fuel core. In addition, the facility offers a powerful means to test and validate astronomical models and computations in the laboratory. As well as demonstrating the ability to recreate extreme astronomical conditions by the facilities, our theoretical description of the laboratory experiment was compared with the generally accepted explanation

  15. Alterations in intracellular ionic calcium levels in isolated adult rat cardiac myocytes due to the generation of free radicals

    Energy Technology Data Exchange (ETDEWEB)

    Burton, K.P.; Nazeran, H.; Hagler, H.K. (Univ. of Texas, Dallas, TX (United States))

    1991-03-15

    Oxygen-derived free radical production has been documented to occur on reperfusion of the ischemic myocardium. Intracellular ionic calcium ((Ca{sup ++}){sub i}) levels in isolated adult rat cardiac myocytes (M) exposed to free radicals were evaluated using the fluorescent calcium indicator, fura-2. The effect of different time periods of free radical exposure and the level of extracellular Ca{sup ++} concentration on altering (Ca{sup ++}){sub i} was examined. The free radical generating system (FRGS) utilized consisted of a HEPES buffered physiological salt solution containing 2.3 mM purine, 2.4. {mu}M iron-loaded transferrin and 0.01 U/ml xanthine oxidase. M maintained in HEPES buffer or the HEPES buffer containing purine and iron-loaded transferrin continued to stimulate, exhibited relatively uniform 340/380 ratios and maintained a rod shape for extended time periods. M continuously exposed to the FRGS showed a significant increase in (Ca{sup ++}){sub i}, became unresponsive to stimulation at 31 {plus minus} 7 (SE) min and eventually exhibited contracture. Exposure to the FRGS for 10 min resulted in a response similar to continuous exposure. M exposed to the FRGS for 5 min exhibited regular Ca{sup ++} transients for 55{plus minus}5 min. M exposed to the FRGS for 10 min and maintained in 2.5 mM Ca{sup ++} versus 1.25 mM Ca{sup ++}, accumulated significantly higher (CA{sup ++}){sub i}. Quiescent myocytes continuously exposed to the FRGS also exhibited a significant increase in (Ca{sup ++}){sub i} over time. Thus, a brief period of free radical exposure may induce subsequent damage. Alterations in Ca{sup ++} flux resulting from the generation of free radicals may possibly contribute to the development of Ca{sup ++} overload and myocardial arrhythmias.

  16. Environmental Test Facility (ETF)

    Data.gov (United States)

    Federal Laboratory Consortium — The Environmental Test Facility (ETF) provides non-isolated shock testing for stand-alone equipment and full size cabinets under MIL-S-901D specifications. The ETF...

  17. An Effective Strategy for Reliably Isolating Heritable and Cas9-Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing.

    Science.gov (United States)

    Gao, Xiuhua; Chen, Jilin; Dai, Xinhua; Zhang, Da; Zhao, Yunde

    2016-07-01

    Mutations generated by CRISPR/Cas9 in Arabidopsis (Arabidopsis thaliana) are often somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can ensure the stable transmission of the identified mutations to next generations, but the process is laborious and inefficient. Here, we present a simple visual screen for Cas9-free T2 seeds, allowing us to quickly obtain Cas9-free Arabidopsis mutants in the T2 generation. To demonstrate this in principle, we targeted two sites in the AUXIN-BINDING PROTEIN1 (ABP1) gene, whose function as a membrane-associated auxin receptor has been challenged recently. We obtained many T1 plants with detectable mutations near the target sites, but only a small fraction of T1 plants yielded Cas9-free abp1 mutations in the T2 generation. Moreover, the mutations did not segregate in Mendelian fashion in the T2 generation. However, mutations identified in the Cas9-free T2 plants were stably transmitted to the T3 generation following Mendelian genetics. To further simplify the screening procedure, we simultaneously targeted two sites in ABP1 to generate large deletions, which can be easily identified by PCR. We successfully generated two abp1 alleles that contained 1,141- and 711-bp deletions in the ABP1 gene. All of the Cas9-free abp1 alleles we generated were stable and heritable. The method described here allows for effectively isolating Cas9-free heritable CRISPR mutants in Arabidopsis.

  18. Generation of germ-line chimera zebrafish using primordial germ cells isolated from cultured blastomeres and cryopreserved embryoids.

    Science.gov (United States)

    Kawakami, Yutaka; Goto-Kazeto, Rie; Saito, Taiju; Fujimoto, Takafumi; Higaki, Shogo; Takahashi, Yoshiyuki; Arai, Katsutoshi; Yamaha, Etsuro

    2010-01-01

    Primordial germ cells (PGCs) are the only cells in developing embryos with the potential to transmit genetic information to the next generation. In our previous study, a single PGC transplanted into a host differentiated into fertile gametes and produced germ-line chimeras of cyprinid fish, including zebrafish. In this study, we aimed to induce germ-line chimeras by transplanting donor PGCs from various sources (normal embryos at different stages, dissociated blastomeres, embryoids, or embryoids cryopreserved by vitrification) into host blastulae, and compare the migration rates of the PGCs towards the gonadal ridge. Isolated, cultured blastomeres not subject to mesodermal induction were able to differentiate into PGCs that retained their motility. Moreover, these PGCs successfully migrated towards the gonadal ridge of the host and formed viable gametes. Motility depended on developmental stage and culture duration: PGCs obtained at earlier developmental stages and with shorter cultivation periods showed an increased rate of migration to the gonadal ridge. Offspring were obtained from natural spawning between normal females and chimeric males. These results provide the basis for new methods of gene preservation in zebrafish.

  19. Facile synthesis of polypyrrole functionalized nickel foam with catalytic activity comparable to Pt for the poly-generation of hydrogen and electricity

    Science.gov (United States)

    Tang, Tiantian; Li, Kan; Shen, Zhemin; Sun, Tonghua; Wang, Yalin; Jia, Jinping

    2016-01-01

    Polypyrrole functionalized nickel foam is facilely prepared through the potentiostatic electrodeposition. The PPy-functionalized Ni foam functions as a hydrogen-evolution cathode in a rotating disk photocatalytic fuel cell, in which hydrogen energy and electric power are generated by consuming organic wastes. The PPy-functionalized Ni foam cathode exhibits stable catalytic activities after thirteen continuous runs. Compared with net or plate structure, the Ni foam with a unique three-dimensional reticulate structure is conducive to the electrodeposition of PPy. Compared with Pt-group electrode, PPy-coated Ni foam shows a satisfactory catalytic performance for the H2 evolution. The combination of PPy and Ni forms a synergistic effect for the rapid trapping and removal of proton from solution and the catalytic reduction of proton to hydrogen. The PPy-functionalized Ni foam could be applied in photocatalytic and photoelectrochemical generation of H2. In all, we report a low cost, high efficient and earth abundant PPy-functionalized Ni foam with a satisfactory catalytic activities comparable to Pt for the practical application of poly-generation of hydrogen and electricity.

  20. A mobile test facility based on a magnetic cumulative generator to study the stability of the power plants under impact of lightning currents

    Science.gov (United States)

    Shurupov, A. V.; Zavalova, V. E.; Kozlov, A. V.; Shurupov, M. A.

    2016-12-01

    The report presents the results of the development and field testing of a mobile test facility based on a helical magnetic cumulative generator (MCGTF). The system is designed for full-scale modeling of lightning currents to study the safety of power plants of any type, including nuclear power plants. Advanced technologies of high-energy physics for solving both engineering and applied problems underlie this pilot project. The energy from the magnetic cumulative generator (MCG) is transferred to a high-impedance load with high efficiency of more than 50% using pulse transformer coupling. Modeling of the dynamics of the MEG that operates in a circuit with lumped parameters allows one to apply the law of inductance output during operation of the MCG, thus providing the required front of the current pulse in the load without using any switches. The results of field testing of the MCGTF are presented for both the ground loop and the model load. The ground loop generates a load resistance of 2-4 Ω. In the tests, the ohmic resistance of the model load is 10 Ω. It is shown that the current pulse parameters recorded in the resistive-inductive load are close to the calculated values.

  1. A mobile test facility based on a magnetic cumulative generator to study the stability of the power plants under impact of lightning currents

    Energy Technology Data Exchange (ETDEWEB)

    Shurupov, A. V.; Zavalova, V. E., E-mail: zavalova@fites.ru; Kozlov, A. V.; Shurupov, M. A. [Russian Academy of Sciences, Joint Institute for High Temperatures (Russian Federation)

    2016-12-15

    The report presents the results of the development and field testing of a mobile test facility based on a helical magnetic cumulative generator (MCGTF). The system is designed for full-scale modeling of lightning currents to study the safety of power plants of any type, including nuclear power plants. Advanced technologies of high-energy physics for solving both engineering and applied problems underlie this pilot project. The energy from the magnetic cumulative generator (MCG) is transferred to a high-impedance load with high efficiency of more than 50% using pulse transformer coupling. Modeling of the dynamics of the MEG that operates in a circuit with lumped parameters allows one to apply the law of inductance output during operation of the MCG, thus providing the required front of the current pulse in the load without using any switches. The results of field testing of the MCGTF are presented for both the ground loop and the model load. The ground loop generates a load resistance of 2–4 Ω. In the tests, the ohmic resistance of the model load is 10 Ω. It is shown that the current pulse parameters recorded in the resistive-inductive load are close to the calculated values.

  2. Investigating the Threshold and Strength of Emission Lines Generated by Magnetized Stimulated Brillouin Scatter (MSBS) using HAARP facilities

    Science.gov (United States)

    Mahmoudian, A.; Scales, W.; Bernhardt, P. A.

    2011-12-01

    The High-Frequency Active Auroral Research Program (HAARP) in Gakona, Alaska provides effective radiated powers in the megawatt range that have allowed researchers to study many non-linear effects of wave-plasma interactions. Stimulated Electromagnetic Emission (SEE) is of interest to the ionospheric community for its diagnostic purposes. In recent HAARP heating experiments, it has been shown that during the Magnetized Stimulated Brillouin Scattering MSBS instability, the pumped electromagnetic wave may decay into an electromagnetic wave and a low frequency electrostatic wave (either ion acoustic IA wave or electrostatic ion cyclotron EIC wave). According to the matching conditions, the O-mode electromagnetic wave can excite either an ion-acoustic wave with a frequency less than the ion cyclotron frequency that propagates along the magnetic field or an electrostatic ion cyclotron (EIC) wave with frequency just above the ion cyclotron frequency that propagates at an angle with respect to the magnetic field. Using Stimulated Electromagnetic Emission (SEE) spectral features, side bands which extend above and below the pump frequency can yield significant diagnostics for the modified ionosphere. It has been shown that the IA wave frequency offsets can be used to measure electron temperature in the heated ionosphere and EIC wave offsets can be used as a sensitive method to determine the ion species by measuring ion mass using the ion gyro-frequency offset. In this presentation the results of SEE experiment at 2010 PARS summer school and 2011 SSRC will be discussed. The experiment was performed at the 3rd electron gyro harmonic with frequency sweeping, power stepping and beam angle variation. Three diagnostics were implemented to study the SEE. There were 1) A 4 channel spectrum analyzer SEE receiver, 2) the University of Alaska SuperDARN radar facility and, 3) the MUIR incoherent scatter radar. The experimental results aimed to show the threshold for transmitter power

  3. Analysis of accident sequences and source terms at treatment and storage facilities for waste generated by US Department of Energy waste management operations

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, C.; Nabelssi, B.; Roglans-Ribas, J.; Folga, S.; Policastro, A.; Freeman, W.; Jackson, R.; Mishima, J.; Turner, S.

    1996-12-01

    This report documents the methodology, computational framework, and results of facility accident analyses performed for the US Department of Energy (DOE) Waste Management Programmatic Environmental Impact Statement (WM PEIS). The accident sequences potentially important to human health risk are specified, their frequencies assessed, and the resultant radiological and chemical source terms evaluated. A personal-computer-based computational framework and database have been developed that provide these results as input to the WM PEIS for the calculation of human health risk impacts. The WM PEIS addresses management of five waste streams in the DOE complex: low-level waste (LLW), hazardous waste (HW), high-level waste (HLW), low-level mixed waste (LLMW), and transuranic waste (TRUW). Currently projected waste generation rates, storage inventories, and treatment process throughputs have been calculated for each of the waste streams. This report summarizes the accident analyses and aggregates the key results for each of the waste streams. Source terms are estimated, and results are presented for each of the major DOE sites and facilities by WM PEIS alternative for each waste stream. Key assumptions in the development of the source terms are identified. The appendices identify the potential atmospheric release of each toxic chemical or radionuclide for each accident scenario studied. They also discuss specific accident analysis data and guidance used or consulted in this report.

  4. A state-of-the-art report on the off-gas treatment technology generated from the nuclear facilities

    Energy Technology Data Exchange (ETDEWEB)

    Oh, Won Zin; Lee, Hoo Geun; Park, Geun Il; Choi, Byung Seon; Lee, Kee Won; Cho, Il Hoon; Kim, Jung Kook; Park, Hyun Soo

    1997-10-01

    This state-of-the-art report describes various technologies for offgas treatment. It provides comprehensive treatment technologies of the extensive subject such as particulates, radioactive iodine, carbon dioxide, Kr/Xe and Cs/Ru. This report also incorporates the wastes generation and its characteristics as well as the historical and current management practices. A number of review articles by experts in various area of concern and some of the removal systems that have been designed for power plants and, particularly, for spent fuel reusing plants are also involved. As a result, it can be drawn that the drying processes for offgas treatment have much benefits in standpoints of simplicity, economy, disposal safety and resource reuse rather than the wet processes. (author). 226 refs., 38 tabs., 44 figs

  5. Updates to the Generation of Physics Data Inputs for MAMMOTH Simulations of the Transient Reactor Test Facility - FY2016

    Energy Technology Data Exchange (ETDEWEB)

    Ortensi, Javier [Idaho National Lab. (INL), Idaho Falls, ID (United States); Baker, Benjamin Allen [Idaho National Lab. (INL), Idaho Falls, ID (United States); Schunert, Sebastian [Idaho National Lab. (INL), Idaho Falls, ID (United States); Wang, Yaqi [Idaho National Lab. (INL), Idaho Falls, ID (United States); Gleicher, Frederick Nathan [Idaho National Lab. (INL), Idaho Falls, ID (United States); DeHart, Mark David [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-06-01

    The INL is currently evolving the modeling and simulation (M&S) capability that will enable improved core operation as well as design and analysis of TREAT experiments. This M&S capability primarily uses MAMMOTH, a reactor physics application being developed under Multi-physics Object Oriented Simulation Environment (MOOSE) framework. MAMMOTH allows the coupling of a number of other MOOSE-based applications. This second year of work has been devoted to the generation of a deterministic reference solution for the full core, the preparation of anisotropic diffusion coefficients, the testing of the SPH equivalence method, and the improvement of the control rod modeling. In addition, this report includes the progress made in the modeling of the M8 core configuration and experiment vehicle since January of this year.

  6. Facile Preparation of Highly Conductive Metal Oxides by Self-Combustion for Solution-Processed Thermoelectric Generators.

    Science.gov (United States)

    Kang, Young Hun; Jang, Kwang-Suk; Lee, Changjin; Cho, Song Yun

    2016-03-01

    Highly conductive indium zinc oxide (IZO) thin films were successfully fabricated via a self-combustion reaction for application in solution-processed thermoelectric devices. Self-combustion efficiently facilitates the conversion of soluble precursors into metal oxides by lowering the required annealing temperature of oxide films, which leads to considerable enhancement of the electrical conductivity of IZO thin films. Such enhanced electrical conductivity induced by exothermic heat from a combustion reaction consequently yields high performance IZO thermoelectric films. In addition, the effect of the composition ratio of In to Zn precursors on the electrical and thermoelectric properties of the IZO thin films was investigated. IZO thin films with a composition ratio of In:Zn = 6:2 at the low annealing temperature of 350 °C showed an enhanced electrical conductivity, Seebeck coefficient, and power factor of 327 S cm(-1), 50.6 μV K(-1), and 83.8 μW m(-1) K(-2), respectively. Moreover, the IZO thin film prepared at an even lower temperature of 300 °C retained a large power factor of 78.7 μW m(-1) K(-2) with an electrical conductivity of 168 S cm(-1). Using the combustive IZO precursor, a thermoelectric generator consisting of 15 legs was fabricated by a printing process. The thermoelectric array generated a thermoelectric voltage of 4.95 mV at a low temperature difference (5 °C). We suggest that the highly conductive IZO thin films by self-combustion may be utilized for fabricating n-type flexible printed thermoelectric devices.

  7. Large-scale experimental facility for emergency condition investigation of a new generation NPP WWER-640 reactor with passive safety systems

    Energy Technology Data Exchange (ETDEWEB)

    Aniskevich, Y.N.; Vasilenko, V.A.; Zasukha, V.K.; Migrov, Y.A.; Khabensky, V.B. [Research Inst. of Technology NITI (Russian Federation)

    1997-12-31

    The creation of the large-scale integral experimental facility (KMS) is specified by the programme of the experimental investigations to justify the engineering decisions on the safety of the design of the new generation NPP with the reactor WWER-640. The construction of KMS in a full volume will allow to conduct experimental investigations of all physical phenomena and processes, practically, occurring during the accidents on the NPPs with the reactor of WWER type and including the heat - mass exchange processes with low rates of the coolant, which is typical during the utilization of the passive safety systems, process during the accidents with a large leak, and also the complex intercommunicated processes in the reactor unit, passive safety systems and in the containment with the condition of long-term heat removal to the final absorber. KMS is being constructed at the Research Institute of Technology (NITI), Sosnovy Bor, Leningrad region, Russia. (orig.). 5 refs.

  8. Scattering chamber facility for double-differential cross-section measurement with 14 MeV DT neutron generator at IPR

    Indian Academy of Sciences (India)

    PRAJAPATI P M; PANDEY BHAWNA; GUPTA N C; KUMAR SURESH; NAYAK B K; SAXENA A; SURYANARAYANA S V; JAKHAR S; VALA SUDHIRSINH; RAO C V S; BASU T K

    2016-06-01

    Measurement of double-differential cross-sections of 14 MeV neutron-inducedcharged-particle productions is very important for estimating the nuclear heating and radiation damage of a fusion reactor. Only a few experimental data are available even though the nuclear reaction cross-section data of structural materials are important in fusion nuclear technology. In this context,general purpose scattering chamber facility has been developed for accelerator-based 14 MeV DT neutron generator to measure double-differential nuclear reaction cross-section at Fusion Neutronics Laboratory, IPR. It has been designed for experiments using silicon surface barrier detectors forthe online detection of charged particles. It offers flexibility in the arrangement of silicon surface barrier detectors.

  9. Preliminary report for analysis of genome wide mutations from four ciprofloxacin resistant B. anthracis Sterne isolates generated by Illumina, 454 sequencing and microarrays for DHS

    Energy Technology Data Exchange (ETDEWEB)

    Jaing, Crystal [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Vergez, Lisa [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hinckley, Aubree [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Thissen, James [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Gardner, Shea [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); McLoughlin, Kevin [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Jackson, Paul [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Ellingson, Sally [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hauser, Loren [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brettin, Tom [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Fofanov, Viacheslav [Eureka Genomics, Hercules, CA (United States); Koshinsky, Heather [Eureka Genomics, Hercules, CA (United States); Fofanov, Yuriy [Univ. of Houston, TX (United States)

    2011-06-21

    The objective of this project is to provide DHS a comprehensive evaluation of the current genomic technologies including genotyping, Taqman PCR, multiple locus variable tandem repeat analysis (MLVA), microarray and high-throughput DNA sequencing in the analysis of biothreat agents from complex environmental samples. As the result of a different DHS project, we have selected for and isolated a large number of ciprofloxacin resistant B. anthracis Sterne isolates. These isolates vary in the concentrations of ciprofloxacin that they can tolerate, suggesting multiple mutations in the samples. In collaboration with University of Houston, Eureka Genomics and Oak Ridge National Laboratory, we analyzed the ciprofloxacin resistant B. anthracis Sterne isolates by microarray hybridization, Illumina and Roche 454 sequencing to understand the error rates and sensitivity of the different methods. The report provides an assessment of the results and a complete set of all protocols used and all data generated along with information to interpret the protocols and data sets.

  10. Regional Issue Identification and Assessment (RIIA). Volume III. Institutional barriers to developing power generation facilities in the Pacific Northwest

    Energy Technology Data Exchange (ETDEWEB)

    Morris, F. A.; Sawyer, C. H.; Maxwell, J. H.

    1979-10-01

    The Regional Assessments Division in the US Department of Energy (DOE) has undertaken a program to assess the probable consequences of various national energy policies in regions of the United States and to evaluate the constraints on national energy policy imposed by conditions in these regions. The program is referred to as the Regional Issues Identification and Assessment (RIIA) Program. Currently the RIIA Program is evaluating the Trendlong Mid-Mid scenario, a pattern of energy development for 1985 and 1990 derived from the Project Independence Evaluation System (PIES) model. This scenario assumes a medium annual growth rate in both the national demand for and national supply of energy. It has been disaggregated to specify the generating capacity to be supplied by each energy source in each state. Pacific Northwest Laboratory (PNL) has the responsibility for evaluating the scenario for the Federal Region 10, consisting of Alaska, Idaho, Oregon, and Washington. PNL is identifying impacts and constraints associated with realizing the scenario in a variety of categories, including air and water quality impacts, health and safety effects, and socioeconomic impacts. This report summarizes the analysis of one such category: institutional constraints - defined to include legal, organizational, and political barriers to the achievement of the scenario in the Northwest.

  11. Structural changes and biological responsiveness of an injectable and mouldable monetite bone graft generated by a facile synthetic method.

    Science.gov (United States)

    Cama, G; Gharibi, B; Knowles, J C; Romeed, S; DiSilvio, L; Deb, S

    2014-12-06

    Brushite (dicalcium phosphate dihydrate) and monetite (dicalcium phosphate anhydrous) are of considerable interest in bone augmentation owing to their metastable nature in physiological fluids. The anhydrous form of brushite, namely monetite, has a finer microstructure with higher surface area, strength and bioresorbability, which does not transform to the poorly resorbable hydroxyapatite, thus making it a viable alternative for use as a scaffold for engineering of bone tissue. We recently reported the formation of monetite cements by a simple processing route without the need of hydrothermal treatment by using a high concentration of sodium chloride in the reaction mix of β-tricalcium phosphate and monocalcium phosphate monohydrate. In this paper, we report the biological responsiveness of monetite formed by this method. The in vitro behaviour of monetite after interaction and ageing both in an acellular and cellular environment showed that the crystalline phase of monetite was retained over three weeks as evidenced from X-ray diffraction measurements. The crystal size and morphology also remained unaltered after ageing in different media. Human osteoblast cells seeded on monetite showed the ability of the cells to proliferate and express genes associated with osteoblast maturation and mineralization. Furthermore, the results showed that monetite could stimulate osteoblasts to undergo osteogenesis and accelerate osteoblast maturation earlier than cells cultured on hydroxyapatite scaffolds of similar porosity. Osteoblasts cultured on monetite cement also showed higher expression of osteocalcin, which is an indicator of the maturation stages of osteoblastogenesis and is associated with matrix mineralization and bone forming activity of osteoblasts. Thus, this new method of fabricating porous monetite can be safely used for generating three-dimensional bone graft constructs.

  12. Analysis of nanometer-isolated trench diffract aerial image of both conventional and second-generation synchrotron-based proximity x-ray lithography

    Science.gov (United States)

    Xie, Changqing; Chen, Dapeng; Liu, Ming; Ye, Tianchun; Yi, Futing

    2005-01-01

    In this paper, Beam Propagation Method (BPM) with Fast Fourier Transforms(FFT) is employed to efficiently calculate the diffract image in the wafer plane for both conventional and second generation synchrotron-based proximity x-ray lithography(PXL). In the simulation, a dark-field isolated space pattern silicon nitride/Ta x-ray mask is used for conventional PXL and a diamond /Ta x-ray mask is used for second generation PXL, the diffract image"s dependency on absorber thickness, mask-wafer gap, effective total blur, linewidth and absorber sidewall slope has been numerically evaluated. For conventional PXL, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap should be controlled below 5 micron, the optimization condition is mask-wafer gap 5 micron, Ta absorber thickness 300nm, effective total blur 10nm, absorber sidewall slope 3°, the corresponding aerial image contrast is 0.457; For second generation, in order to obtain a isolated trench resolution of 50nm, the mask-wafer gap can be as large as 10 micron. In order to obtain a isolated trench resolution of 35nm, mask-wafer gap should be controlled below 5 micron.

  13. Generation of warm dense matter and strongly coupled plasmas using the High Radiation on Materials facility at the CERN Super Proton Synchrotron

    CERN Document Server

    Tahir, N A; Brugger, M; Assmann, R; Shutov, A; Lomonosov, I V; Gryaznov, V; Piriz, A R; Udrea, S; Hoffmann, D H H; Fortov, V E; Deutsch, C

    2009-01-01

    A dedicated facility named High Radiation on Materials (HiRadMat) is being constructed at CERN to study the interaction of the 450 GeV protons generated by the Super Proton Synchrotron (SPS) with fixed solid targets of different materials. The main purpose of these future experiments is to study the generation and propagation of thermal shock waves in the target in order to assess the damage caused to the equipment, including collimators and absorbers, in case of an accident involving an uncontrolled release of the entire beam at a given point. Detailed numerical simulations of the beam-target interaction of several cases of interest have been carried out. In this paper we present simulations of the thermodynamic and the hydrodynamic response of a solid tungsten cylindrical target that is facially irradiated with the SPS beam with nominal parameters. These calculations have been carried out in two steps. First, the energy loss of the protons is calculated in the solid target using the FLUKA code (Fasso et al....

  14. Component Test Facility (Comtest) Phase 1 Engineering For 760°C (1400°F) Advanced Ultrasupercritical (A-USC) Steam Generator Development

    Energy Technology Data Exchange (ETDEWEB)

    Weitzel, Paul [Babcock & Wilcox Power Generation Group, Inc., Barberton, OH (United States)

    2016-05-13

    The Babcock & Wilcox Company (B&W) performed a Pre-Front End Engineering Design (Pre-FEED) of an A-USC steam superheater for a proposed component test program achieving 760°C (1400°F) steam temperature. This would lead to follow-on work in a Phase 2 and Phase 3 that would involve detail design, manufacturing, construction and operation of the ComTest. Phase 1 results have provided the engineering data necessary for proceeding to the next phase of ComTest. The steam generator superheater would subsequently supply the steam to an A-USC prototype intermediate pressure steam turbine. The ComTest program is important in that it will place functioning A-USC components in operation and in coordinated boiler and turbine service. It is also important to introduce the power plant operation and maintenance personnel to the level of skills required and provide the first background experience with hands-on training. The project will provide a means to exercise the complete supply chain events required in order to practice and perfect the process for A-USC power plant design, supply, manufacture, construction, commissioning, operation and maintenance. Representative participants will then be able to transfer knowledge and recommendations to the industry. ComTest is conceived in the manner of using a separate standalone plant facility that will not jeopardize the host facility or suffer from conflicting requirements in the host plant’s mission that could sacrifice the nickel alloy components and not achieve the testing goals. ComTest will utilize smaller quantities of the expensive materials and reduce the risk in the first operational practice for A-USC technology in the United States. Components at suitable scale in ComTest provide more assurance before putting them into practice in the full size A-USC demonstration plant.

  15. The HIV-1 V3 domain on field isolates: participation in generation of escape virus in vivo and accessibility to neutralizing antibodies

    DEFF Research Database (Denmark)

    Arendrup, M; Akerblom, L; Heegaard, P M

    1995-01-01

    The V3 domain is highly variable and induces HIV neutralizing antibodies (NA). Here we addressed the issues of 1) the participation of mutations in V3 in generation of neutralization resistant escape virus in vivo and 2) the applicability of synthetic V3 peptides corresponding to field isolates...... to induce neutralizing immune sera. Seven peptides corresponding to the V3 region of primary and escape virus from 3 HIV-1 infected patients were synthesized and used for antibody (Abs) studies and immunizations. The anti-V3 Abs titre in patient serum was generally low against peptides corresponding...... to autologous virus isolated later than the serum sample in contrast to the titre against peptides corresponding to virus isolated earlier than the serum sample. Furthermore, neutralizing anti-V3 monoclonal antibodies (MAbs) raised against V3 peptides from laboratory strains of HIV-1 showed distinct binding...

  16. Identification of new splice sites used for generation of rev transcripts in human immunodeficiency virus type 1 subtype C primary isolates.

    Directory of Open Access Journals (Sweden)

    Elena Delgado

    Full Text Available The HIV-1 primary transcript undergoes a complex splicing process by which more than 40 different spliced RNAs are generated. One of the factors contributing to HIV-1 splicing complexity is the multiplicity of 3' splice sites (3'ss used for generation of rev RNAs, with two 3'ss, A4a and A4b, being most commonly used, a third site, A4c, used less frequently, and two additional sites, A4d and A4e, reported in only two and one isolates, respectively. HIV-1 splicing has been analyzed mostly in subtype B isolates, and data on other group M clades are lacking. Here we examine splice site usage in three primary isolates of subtype C, the most prevalent clade in the HIV-1 pandemic, by using an in vitro infection assay of peripheral blood mononuclear cells. Viral spliced RNAs were identified by RT-PCR amplification using a fluorescently-labeled primer and software analyses and by cloning and sequencing the amplified products. The results revealed that splice site usage for generation of rev transcripts in subtype C differs from that reported for subtype B, with most rev RNAs using two previously unreported 3'ss, one located 7 nucleotides upstream of 3'ss A4a, designated A4f, preferentially used by two isolates, and another located 14 nucleotides upstream of 3'ss A4c, designated A4g, preferentially used by the third isolate. A new 5' splice site, designated D2a, was also identified in one virus. Usage of the newly identified splice sites is consistent with sequence features commonly found in subtype C viruses. These results show that splice site usage may differ between HIV-1 subtypes.

  17. Identification of new splice sites used for generation of rev transcripts in human immunodeficiency virus type 1 subtype C primary isolates.

    Science.gov (United States)

    Delgado, Elena; Carrera, Cristina; Nebreda, Paloma; Fernández-García, Aurora; Pinilla, Milagros; García, Valentina; Pérez-Álvarez, Lucía; Thomson, Michael M

    2012-01-01

    The HIV-1 primary transcript undergoes a complex splicing process by which more than 40 different spliced RNAs are generated. One of the factors contributing to HIV-1 splicing complexity is the multiplicity of 3' splice sites (3'ss) used for generation of rev RNAs, with two 3'ss, A4a and A4b, being most commonly used, a third site, A4c, used less frequently, and two additional sites, A4d and A4e, reported in only two and one isolates, respectively. HIV-1 splicing has been analyzed mostly in subtype B isolates, and data on other group M clades are lacking. Here we examine splice site usage in three primary isolates of subtype C, the most prevalent clade in the HIV-1 pandemic, by using an in vitro infection assay of peripheral blood mononuclear cells. Viral spliced RNAs were identified by RT-PCR amplification using a fluorescently-labeled primer and software analyses and by cloning and sequencing the amplified products. The results revealed that splice site usage for generation of rev transcripts in subtype C differs from that reported for subtype B, with most rev RNAs using two previously unreported 3'ss, one located 7 nucleotides upstream of 3'ss A4a, designated A4f, preferentially used by two isolates, and another located 14 nucleotides upstream of 3'ss A4c, designated A4g, preferentially used by the third isolate. A new 5' splice site, designated D2a, was also identified in one virus. Usage of the newly identified splice sites is consistent with sequence features commonly found in subtype C viruses. These results show that splice site usage may differ between HIV-1 subtypes.

  18. Electric power generation in isolated communities at Amazon, Brazil, from the sustainable biomass: project ENERMAD; A geracao de energia eletrica em comunidades isoladas na Amazonia a partir de biomassa sustentavel: projeto ENERMAD

    Energy Technology Data Exchange (ETDEWEB)

    Velazquez, Silvia Maria Stortini Gonzalez; Santos, Sandra Maria Apolinario; Moreira, Jose Roberto; Coelho, Suani Teixeira [Centro Nacional de Referencia em Biomassa, Sao Paulo, SP (Brazil)], E-mail: silvia@iee.usp.br

    2010-07-01

    The ENERMAD project viewed the electric power generation in isolated communities, from the forestalls residues, to attend the productive activities and residences, besides the steam generation for value aggregation to the community product, reaching to transform the social reality of that community and, to stimulate the investment viability in innovator project for those isolated communities.

  19. Comparison The Effects of Two Monocyte Isolation Methods,Plastic Adherence and Magnetic Activated Cell Sorting Methods,on Phagocytic Activity of Generated Dendritic Cells

    Directory of Open Access Journals (Sweden)

    Behnaz Asadi

    2013-01-01

    Full Text Available Objective: It is believed that monocyte isolation methods and maturation factors affect the phenotypic and functional characteristics of resultant dendritic cells (DC. In the present study, we compared two monocyte isolation methods, including plastic adherence-dendritic cells (Adh-DC and magnetic activated cell sorting- dendritic cells (MACS-DC, and their effects on phagocytic activity of differentiated immature DCs (immDCs.Materials and Methods: In this experimental study, immDCs were generated from plastic adherence and MACS isolated monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF and interleukin 4 (IL-4 in five days. The phagocytic activity of immDCs was analyzed by fluorescein isothiocyanate (FITC-conjugated latex bead using flow cytometry. One way ANOVA test was used for statistical analysis of differences among experimental groups, including Adh-DC and MACS-DC groups.Results: We found that phagocytic activity of Adh-DC was higher than MACS-DC, whereas the mean fluorescence intensity (MFI of phagocytic cells was higher in MACS-DC (p<0.05.Conclusion: We concluded that it would be important to consider phagocytosis parameters of generated DCs before making any decision about monocyte isolation methods to have fully functional DCs.

  20. Health and safety impacts of nuclear, geothermal, and fossil-fuel electric generation in California. Volume 9. Methodologies for review of the health and safety aspects of proposed nuclear, geothermal, and fossil-fuel sites and facilities

    Energy Technology Data Exchange (ETDEWEB)

    Nero, A.V.; Quinby-Hunt, M.S.

    1977-01-01

    This report sets forth methodologies for review of the health and safety aspects of proposed nuclear, geothermal, and fossil-fuel sites and facilities for electric power generation. The review is divided into a Notice of Intention process and an Application for Certification process, in accordance with the structure to be used by the California Energy Resources Conservation and Development Commission, the first emphasizing site-specific considerations, the second examining the detailed facility design as well. The Notice of Intention review is divided into three possible stages: an examination of emissions and site characteristics, a basic impact analysis, and an assessment of public impacts. The Application for Certification review is divided into five possible stages: a review of the Notice of Intention treatment, review of the emission control equipment, review of the safety design, review of the general facility design, and an overall assessment of site and facility acceptability.

  1. Comparative study on the pathogenesis of the generated 9a5b Newcastle disease virus mutant isolate between chickens and waterfowl.

    Science.gov (United States)

    Anis, Z; Morita, T; Azuma, K; Ito, H; Ito, T; Shimada, A

    2013-07-01

    Lentogenic Newcastle disease viruses (NDVs), circulating among waterfowl, have the potential to become highly pathogenic by replication in chickens. The pathological studies that compare NDV infections in chickens and waterfowl are rare. The virulent 9a5b mutant NDV isolate was generated by passaging the lentogenic Goose/Alaska/415/91 NDV isolate in chickens. The pathogenesis of the virulent 9a5b mutant isolate is unknown in both chickens and waterfowl. In this study, the virulent 9a5b mutant NDV isolate was inoculated intranasally in 32-day-old specific pathogen-free white Leghorn chickens and Japanese commercial ducks. Unlike ducks, which remained clinically normal throughout the study, chickens had depression, gasping, oral discharges, and greenish-white soft feces. Gross and histologic lesion patterns as well as viral replication supported the differing clinical outcome. Ducks had slight inflammation mainly in respiratory and digestive tracts, whereas slight nonpurulent encephalitis, necrotizing pancreatitis, tubulointerstitial nephritis, and mild inflammation in respiratory and digestive tracts were detected in chickens. In agreement, interferon-beta (IFN-β)-immunopositive signals were more intense in lung tissue of ducks than that of chickens, and NDV replications were detected intensively in chicken tissues. These results suggest that the 9a5b mutant NDV isolate is more virulent in chickens, and slight histological lesions were induced in ducks even with virulent NDVs.

  2. Implementation of flowsheet change to minimize hydrogen and ammonia generation during chemical processing of high level waste in the defense waste processing facility

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, Dan P. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Woodham, Wesley H. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Williams, Matthew S. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Newell, J. David [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Luther, Michelle C. [Auburn Univ., AL (United States); Brandenburg, Clayton H. [Univ.of South Carolina, Columbia, SC (United States)

    2016-09-27

    Testing was completed to develop a chemical processing flowsheet for the Defense Waste Processing Facility (DWPF), designed to vitrify and stabilize high level radioactive waste. DWPF processing uses a reducing acid (formic acid) and an oxidizing acid (nitric acid) to rheologically thin the slurry and complete the necessary acid base and reduction reactions (primarily mercury and manganese). Formic acid reduces mercuric oxide to elemental mercury, allowing the mercury to be removed during the boiling phase of processing through steam stripping. In runs with active catalysts, formic acid can decompose to hydrogen and nitrate can be reduced to ammonia, both flammable gases, due to rhodium and ruthenium catalysis. Replacement of formic acid with glycolic acid eliminates the generation of rhodium- and ruthenium-catalyzed hydrogen and ammonia. In addition, mercury reduction is still effective with glycolic acid. Hydrogen, ammonia and mercury are discussed in the body of the report. Ten abbreviated tests were completed to develop the operating window for implementation of the flowsheet and determine the impact of changes in acid stoichiometry and the blend of nitric and glycolic acid as it impacts various processing variables over a wide processing region. Three full-length 4-L lab-scale simulations demonstrated the viability of the flowsheet under planned operating conditions. The flowsheet is planned for implementation in early 2017.

  3. Implementation of flowsheet change to minimize hydrogen and ammonia generation during chemical processing of high level waste in the Defense Waste Processing Facility

    Energy Technology Data Exchange (ETDEWEB)

    Lambert, Dan P. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Woodham, Wesley H. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Williams, Matthew S. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Newell, J. David [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Luther, Michelle C. [Auburn Univ., AL (United States); Brandenburg, Clayton H. [Univ.of South Carolina, Columbia, SC (United States)

    2016-09-27

    Testing was completed to develop a chemical processing flowsheet for the Defense Waste Processing Facility (DWPF), designed to vitrify and stabilize high level radioactive waste. DWPF processing uses a reducing acid (formic acid) and an oxidizing acid (nitric acid) to rheologically thin the slurry and complete the necessary acid base and reduction reactions (primarily mercury and manganese). Formic acid reduces mercuric oxide to elemental mercury, allowing the mercury to be removed during the boiling phase of processing through steam stripping. In runs with active catalysts, formic acid can decompose to hydrogen and nitrate can be reduced to ammonia, both flammable gases, due to rhodium and ruthenium catalysis. Replacement of formic acid with glycolic acid eliminates the generation of rhodium- and ruthenium-catalyzed hydrogen and ammonia. In addition, mercury reduction is still effective with glycolic acid. Hydrogen, ammonia and mercury are discussed in the body of the report. Ten abbreviated tests were completed to develop the operating window for implementation of the flowsheet and determine the impact of changes in acid stoichiometry and the blend of nitric and glycolic acid as it impacts various processing variables over a wide processing region. Three full-length 4-L lab-scale simulations demonstrated the viability of the flowsheet under planned operating conditions. The flowsheet is planned for implementation in early 2017.

  4. Facilities & Leadership

    Data.gov (United States)

    Department of Veterans Affairs — The facilities web service provides VA facility information. The VA facilities locator is a feature that is available across the enterprise, on any webpage, for the...

  5. Manassantin A isolated from Saururus chinensis inhibits 5-lipoxygenase-dependent leukotriene C4 generation by blocking mitogen-activated protein kinase activation in mast cells.

    Science.gov (United States)

    Kim, Su Jeong; Lu, Yue; Kwon, Okyun; Hwangbo, Kyoung; Seo, Chang-Seob; Lee, Seung Ho; Kim, Cheorl-Ho; Chang, Young-Chae; Son, Jong Keun; Chang, Hyeun Wook

    2011-01-01

    In this study, manassantin A (Man A), an herbal medicine isolated from Saururus chinensis (S. chinensis), markedly inhibited 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generation in bone marrow-derived mast cells (BMMCs) in a concentration-dependent manner. To investigate the molecular mechanisms underlying the inhibition of LTC(4) generation by Man A, we assessed the effects of Man A on phosphorylation of cytosolic phospholipase A(2) (cPLA(2)) and mitogen-activated protein kinases (MAPKs). Inhibition of LTC(4) generation by Man A was accompanied by a decrease in cPLA(2) phosphorylation, which occurred via the MAPKs including extracellular signal-regulated protein kinase-1/2 (ERK1/2) as well as p38 and c-Jun N-terminal kinase (JNK) pathways. Taken together, the present study suggests the Man A represents a potential therapeutic approach for the treatment of airway allergic-inflammatory diseases.

  6. Biochemistry Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Biochemistry Facility provides expert services and consultation in biochemical enzyme assays and protein purification. The facility currently features 1) Liquid...

  7. Generation of human pluripotent stem cell reporter lines for the isolation of and reporting on astrocytes generated from ventral midbrain and ventral spinal cord neural progenitors

    Directory of Open Access Journals (Sweden)

    Staffan Holmqvist

    2015-07-01

    Full Text Available Astrocytes play a critical role during the development and the maintenance of the CNS in health and disease. Yet, their lack of accessibility from fetuses and from the brain of diseased patients has hindered our understanding of their full implication in developmental and pathogenic processes. Human pluripotent stem cells (PSCs are an alternative source to obtain large quantities of astrocytes in vitro, for mechanistic studies of development and disease. However, these studies often require highly pure populations of astrocytes, which are not always achieved, depending on the PSC lines and protocols used. Here, we describe the generation and characterization of human PSC reporter lines expressing TagRFP driven by the ABC1D region of the human GFAP promoter, as new cellular model for generating homogenous population of astrocytes generated from CNS regionally defined PSC-derived neural progenitors. GFAABC1D::TagRFP-expressing astrocytes can be purified by fluorescent-activated cell sorting and maintain a bright expression for several additional weeks. These express canonical astrocyte markers NF1A, S100β, CX43, GLAST, GS and CD44. These new cellular models, from which highly pure populations of fluorescence-expressing astrocytes can be obtained, provide a new platform for studies where pure or fluorescently labeled astrocyte populations are necessary, for example to assess pro-inflammatory cytokine and chemokine release in response to specific treatment, and uptake and degradation of fluorescently labeled pathogenic proteins, as reported in this study.

  8. Molecular Epidemiology of Extended-Spectrum β-Lactamases among Escherichia coli Isolates Collected in a Swedish Hospital and Its Associated Health Care Facilities from 2001 to 2006▿

    Science.gov (United States)

    Fang, Hong; Ataker, Ferda; Hedin, Göran; Dornbusch, Kathrine

    2008-01-01

    The genetic characteristics and molecular epidemiology of extended-spectrum β-lactamases (ESBLs) among Escherichia coli isolates were investigated at a general hospital and its associated health care facilities in Stockholm, Sweden, during the period from 2001 to 2006. Of 87 consecutive nonduplicate ESBL-positive isolates, 80 isolates encoded CTX-M-type ESBLs, 64 of which were group 1 enzymes. TEM-type and OXA-type β-lactamases were encoded in 63 and 59% of the ESBL isolates, respectively. Pulsed-field gel electrophoresis (PFGE) analysis revealed 40 different pulsotypes, consisting of 11 clones accounting for 66% of all isolates, and 29 unique patterns. Moreover, of the 11 clones, clones 1 and 4 comprised half of the clonally related isolates (28 of 57). Clone 1 was a persistent endemic clone in the area throughout the years, and clone 4 emerged in 2003. However, in recent years, clone 1 isolates were no longer predominant and were gradually replaced by new emerging strains. Concerning β-lactamase gene profiles in relation to PFGE pulsotypes, clone-related bla profiles were observed in certain clones, while in most cases different bla profiles could be observed in the same clone, and the same bla profile could be present in different clones. The molecular epidemiology of ESBL-positive E. coli in the area shows shifts in predominant strains and increased clonal diversity over time. The study also indicated that both clonal spread of epidemic strains and transfer of transposable genetic elements might contribute to the proliferation of ESBLs. PMID:18094139

  9. Antimicrobial resistance of Neisseria gonorrhoeae isolates in south-west Germany, 2004 to 2015: increasing minimal inhibitory concentrations of tetracycline but no resistance to third-generation cephalosporins.

    Science.gov (United States)

    Regnath, Thomas; Mertes, Thomas; Ignatius, Ralf

    2016-09-01

    Increasing antimicrobial resistance of Neisseria gonorrhoeae, particularly to third-generation cephalosporins, has been reported in many countries. We examined the susceptibility (determined by Etest and evaluated using the breakpoints of the European Committee on Antimicrobial Susceptibility Testing) of 434 N. gonorrhoeae isolates collected from 107 female and 327 male patients in Stuttgart, south-west Germany, between 2004 and 2015. During the study period, high proportions of isolates were resistant to ciprofloxacin (70.3%), tetracycline (48.4%; increasing from 27.5% in 2004/2005 to 57.7% in 2014/2015; p = 0.0002) and penicillin (25.6%). The proportion of isolates resistant to azithromycin was low (5.5%) but tended to increase (p = 0.08). No resistance and stable minimum inhibitory concentrations were found for cefixime, ceftriaxone, and spectinomycin. High-level resistance was found for ciprofloxacin (39.6%) and tetracycline (20.0%) but not for azithromycin; 16.3% of the isolates produced betalactamase. Thus, cephalosporins can still be used for the treatment of gonorrhoea in the study area. To avoid further increasing resistance to azithromycin, its usage should be limited to patients allergic to cephalosporins, or (in combination with cephalosporins) to patients for whom no susceptibility testing could be performed or those co-infected with chlamydiae.

  10. US/USSR cooperative program in open-cycle MHD electrical power gneration. Joint test report No. 2: tests in the U-25B facility; MHD generator test No. 3

    Energy Technology Data Exchange (ETDEWEB)

    Tempelmeyer, K E; Sokolov, Y N [eds.

    1979-04-01

    The third joint test with a Soviet U-25B MHD generator and a US superconducting magnet system (SCMS) was conducted in the Soviet U-25B Facility. The primary objectives of the 3rd test were: (1) to operate the facility and MHD channel over a wider range of test parameters, and (2) to study the performance of all components and systems of the flow train at increased mass flow rates of combustion products (up to 4 kg/s), at high magnetic-field induction (up to 5 T), and high values of the electrical field in the MHD generator. The third test has demonstrated that all components and systems of the U-25B facility performed reliably. The electric power generated by the MHD generaor reached a maximum of 575 kW during this test. The MHD generator was operated under electrical loading conditions for 9 hours, and the combustor for a total of approximately 14 hours. Very high Hall fields (2.1 kV/m) were produced in the MHD channel, with a total Hall voltage of 4.24 kV. A detailed description is given of (1) performance of all components and systems of the U-25B facility, (2) analysis of the thermal, gasdynamic, and electrical characteristics of the MHD generator, (3) results of plasma diagnostic studies, (4) studies of vibrational characteristics of the flow train, (5) fluctuation of electrodynamic and gasdynamic parameters, (6) interaction of the MHD generator with the superconducting magnet, and (7) an operational problem, which terminated the test.

  11. Plasma heating and generation of energetic ions with novel three-ion ICRF scenarios on Alcator C-Mod and JET tokamak facilities

    Science.gov (United States)

    Kazakov, Yevgen

    2016-10-01

    This talk will report the first experimental results of novel three-ion ICRF scenarios (two or more majority ion species and one minority) for plasma heating and generating energetic ions in fusion facilities. The key feature of these scenarios is strong absorption of RF power possible at lower concentrations of minority ions than in two-ion plasmas. Effective plasma heating by injecting a small amount of 3He ions into H-D plasma mixtures with nH /ne 70 % has been successfully demonstrated in Alcator C-Mod and JET tokamaks. In C-Mod, efficient plasma heating was observed for 3He concentrations from 0.4-2%. During the discharges, a strong increase in Alfvén eigenmode activity was found to coincide with the addition of 3He to the H-D plasmas. Even lower 3He concentrations ( 0.2 %) were utilized in recent JET experiments. The potential of the D-(3He) -H scenario for plasma heating and generating MeV-range ions in JET plasmas was confirmed by a set of independent measurements, including stabilization of sawteeth, characteristic γ-ray emission, fast-ion loss detector. Furthermore, toroidal Alfvén eigenmodes with a range of toroidal mode numbers n were detected, which is another indication for the presence of significant population of high-energy 3He ions in a plasma. The discussed mechanism of resonant wave-particle interaction opens up various unexplored opportunities for ICRF system, including new scenarios for plasma heating. Three-ion ICRF scenarios are also relevant for the experimental programme of ITER. The possibility of using intrinsic 9Be impurities as the minority (instead of 3He) was suggested for heating bulk ions in D-T plasmas of JET and ITER, as well as heating trace amounts of 3He and 4He ions in H majority plasmas of ITER. The latest results and simulation comparisons will be presented. On behalf of Alcator C-Mod Team (MIT-PSFC, US) and JET Contributors (Culham, UK). Work supported by the US DOE (C-Mod DE-FC02-99ER54512 and SciDAC DE-FC02-01ER54648

  12. The slope of the initial temperature drop predicts acute pulmonary vein isolation using the second-generation cryoballoon.

    Science.gov (United States)

    Deubner, Nikolas; Greiss, Harald; Akkaya, Ersan; Zaltsberg, Sergey; Hain, Andreas; Berkowitsch, Alexander; Güttler, Norbert; Kuniss, Malte; Neumann, Thomas

    2017-09-01

    There is no objective, early indicator of occlusion quality, and efficacy of cryoballoon pulmonary vein isolation. As previous experience suggests that the initial cooling rate correlates with these parameters, we investigated the slope of the initial temperature drop as an objective measure. A systematic evaluation of 523 cryoapplications in 105 patients using a serial ROC-AUC analysis was performed. We found the slope of a linear regression of the temperature-time function to be a good predictor (PPV 0.9, specificity 0.72, sensitivity 0.71, and ROC-AUC 0.75) of acute isolation. It also correlated with nadir temperatures (P< 0.001, adjusted R2= 0.43), predicted very low nadir temperatures, and varied according to visual occlusion grades (ANOVA P< 0.001). About 25 s after freeze initiation, the temperature-time slope predicts important key characteristics of a cryoablation, such as nadir temperature. The slope is the only reported predictor to actually precede acute isolation and thus to support decisions about pull-down manoeuvres or aborting a cryoablation early on. It is also predictive of very low nadir temperatures and phrenic nerve palsy and thus may add to patient safety.

  13. Isolation of expressed sequences encoded by the human Xq terminal portion using microclone probes generated by laser microdissection

    Energy Technology Data Exchange (ETDEWEB)

    Yokoi, H.; Kang, X. (Univ. of Ottawa Faculty of Medicine, Ontario (Canada)); Hadano, Shinji; Kogi, Mieko; Wakasa, Kyo (Tokai Univ. School of Medicine, Kanagawa (Japan)); Ikeda, J.E. (Univ. of Ottawa Faculty of Medicine, Ontario (Canada) Tokai Univ. School of Medicine, Kanagawa (Japan) National Institute of Agrobiological Resources, Ibaraki (Japan))

    1994-04-01

    The genes that cause a variety of neurologic and neuromuscular disorders have been mapped to the distal region of Xq. In an effort to isolate genes from this area, a regional genomic library of the distal 30% of Xq was constructed from a single metaphase spread by means of laser microdissection and single unique primer-polymerase chain reaction. Using pooled probes of 1000 clones from the genomic library, human brain cDNA libraries were screened for expressed sequences encoded by this region. From the 250,000 cDNA clones screened so far, 10 nonoverlapping sequences that mapped back to the target portion were isolated. The complete nucleotide sequences of these cDNA clones have been determined. Analysis of the sequences indicates that none has significant similarity to previously characterized primate genes. One sequence mapping to Xq27.3-qter contained an open reading frame of 281 amino acids and was expressed in every tissue tested. This gene, as well as others isolated in this manner, may prove to be a candidate gene for heritable disorders mapping to this region. 36 refs., 6 figs., 2 tabs.

  14. Power System Stability of a Small Sized Isolated Network Supplied by a Combined Wind-Pumped Storage Generation System: A Case Study in the Canary Islands

    Directory of Open Access Journals (Sweden)

    Carlos A. Platero

    2012-07-01

    Full Text Available Massive integration of renewable energy sources in electrical power systems of remote islands is a subject of current interest. The increasing cost of fossil fuels, transport costs to isolated sites and environmental concerns constitute a serious drawback to the use of conventional fossil fuel plants. In a weak electrical grid, as it is typical on an island, if a large amount of conventional generation is substituted by renewable energy sources, power system safety and stability can be compromised, in the case of large grid disturbances. In this work, a model for transient stability analysis of an isolated electrical grid exclusively fed from a combination of renewable energy sources has been studied. This new generation model will be installed in El Hierro Island, in Spain. Additionally, an operation strategy to coordinate the generation units (wind, hydro is also established. Attention is given to the assessment of inertial energy and reactive current to guarantee power system stability against large disturbances. The effectiveness of the proposed strategy is shown by means of simulation results.

  15. Characterization of a P1-like bacteriophage carrying CTX-M-27 in Salmonella spp. resistant to third generation cephalosporins isolated from pork in China

    Science.gov (United States)

    Yang, Ling; Li, Wan; Jiang, Gui-Ze; Zhang, Wen-Hui; Ding, Huan-Zhong; Liu, Ya-Hong; Zeng, Zhen-Ling; Jiang, Hong-Xia

    2017-01-01

    The aim of this study was to elucidate the epidemiology of third generation cephalosporin resistant Samonella isolates from pork of a slaughterhouse in China and the features of transferable elements carrying blaCTX-M genes. One hundred and twenty-six (7.3%) Salmonella isolates were identified; S. Derby and S. Rissen were the most two prevalent serotypes. Among these isolates 20 (15.8%) were resistant to third generation cephalosporins and nine of them carried blaCTX-M-27. S1-PFGE and replicon typing of blaCTX-M-27-carrying plasmids showed that seven were untypeable plasmids of about 104 Kb and two were IncP plasmids of about 300 Kb. Complete sequence analysis of one PBRT-untypeable plasmid showed it was a P1-like bateriophage, named SJ46, which contained a non-phage-associated region with several mobile elements, including Tn1721, ISEcp1B and IS903D. The other six 104 Kb PBRT-untypeable blaCTX-M-27-carrying plasmids also harboured the same phage-insertion region of SJ46 suggesting that they were the same P1-like bacteriophage. PFGE profiles of the parental strains revealed both potential vertical and horizontal spread of this P1-like blaCTX-M-27-containing element. Additionally, the representative gene of the P1 family bacteriophage, repL, was detected in 19.0% (24/126) of the isolates. This study indicated a potential role of P1-family bacteriophage in capture and spread of antimicrobial resistance in pathogens. PMID:28098241

  16. LOTIS facility initial operational capabilities: flexible user interfaces

    Science.gov (United States)

    Hutchison, Sheldon B.; Bell, Raymond M., Jr.; Borota, Stephen A.; Cuzner, Gregor J.; Cochrane, Andrew T.

    2010-10-01

    The Large Optical Test and Integration Site (LOTIS) at the Lockheed Martin Space Systems Company in Sunnyvale, CA, has successfully reached Initial Operational Capability (IOC). LOTIS is designed for the verification and testing of optical systems. The facility consists of a large, temperature stabilized vacuum chamber that also functions as a class 10k cleanroom. Within this chamber and atop an advanced vibration-isolation bench are the 6.5 meter diameter LOTIS Collimator and Scene Generator, LOTIS alignment and support equipment. IOC included completion of the entire facility as well as operation of the LOTIS collimator in air. Wavefront properties of the collimator will be described as well as facility vibration isolation properties and turbulence levels within the collimator test chamber. User-specific test capabilities will also be addressed for two major areas of concern.

  17. Clonally related methicillin-resistant Staphylococcus aureus isolated from short-finned pilot whales (Globicephala macrorhynchus), human volunteers, and a bayfront cetacean rehabilitation facility

    Science.gov (United States)

    In May of 2011 a live mass stranding of 26 short-finned pilot whales (Globicephala macrorhynchus) occurred in the lower Florida Keys. Five surviving whales were transferred from the original stranding site to a nearby marine mammal rehabilitation facility where they were constantly attended to by a ...

  18. Blastocystis Isolates from Patients with Irritable Bowel Syndrome and from Asymptomatic Carriers Exhibit Similar Parasitological Loads, but Significantly Different Generation Times and Genetic Variability across Multiple Subtypes.

    Directory of Open Access Journals (Sweden)

    Gie-Bele Vargas-Sanchez

    Full Text Available Blastocystis spp is a common intestinal parasite of humans and animals that has been associated to the etiology of irritable bowel syndrome (IBS; however, some studies have not found this association. Furthermore, many biological features of Blastocystis are little known. The objective of present study was to assess the generation times of Blastocystis cultures, from IBS patients and from asymptomatic carriers. A total of 100 isolates were obtained from 50 IBS patients and from 50 asymptomatic carriers. Up to 50 mg of feces from each participant were cultured in Barret's and in Pavlova's media during 48 h. Initial and final parasitological load were measured by microscopy and by quantitative PCR. Amplicons were purified, sequenced and submitted to GenBank; sequences were analysed for genetic diversity and a Bayesian inference allowed identifying genetic subtypes (ST. Generation times for Blastocystis isolates in both media, based on microscopic measures and molecular assays, were calculated. The clinical symptoms of IBS patients and distribution of Blastocystis ST 1, 2 and 3 in both groups was comparable to previous reports. Interestingly, the group of cases showed scarce mean nucleotide diversity (π as compared to the control group (0.011±0.016 and 0.118±0.177, respectively, whilst high gene flow and small genetic differentiation indexes between different ST were found. Besides, Tajima's D test showed negative values for ST1-ST3. No statistical differences regarding parasitological load between cases and controls in both media, as searched by microscopy and by qPCR, were detected except that parasites grew faster in Barret's than in Pavlova's medium. Interestingly, slow growth of isolates recovered from cases in comparison to those of controls was observed (p<0.05. We propose that generation times of Blastocystis might be easily affected by intestinal environmental changes due to IBS probably because virulent strains with slow growth may be

  19. Validation of electrical ostial pulmonary vein isolation verified with a spiral inner lumen mapping catheter during second-generation cryoballoon ablation.

    Science.gov (United States)

    Miyazaki, Shinsuke; Kajiyama, Takatsugu; Watanabe, Tomonori; Taniguchi, Hiroshi; Nakamura, Hiroaki; Hamaya, Rikuta; Kusa, Shigeki; Igarashi, Miyako; Hachiya, Hitoshi; Hirao, Kenzo; Iesaka, Yoshito

    2017-08-01

    Achieve catheters are cryoballoon guidewires that enable pulmonary vein (PV) potential mapping. The single catheter approach in conjunction with the Achieve catheter is currently standard practice in second-generation cryoballoon ablation, yet circumferential mapping catheters are the gold standard for evaluating PV isolation (PVI). The study sought to validate the ostial PVI verified by an Achieve catheter alone. One hundred fifty-one paroxysmal atrial fibrillation patients undergoing PVI using exclusively 28-mm second-generation cryoballoons were enrolled. PV recordings were analyzed during (real-time recordings) and after cryoballoon applications with 20-mm Achieve mapping catheters, and subsequently validated by 20-mm conventional circumferential mapping catheters. Out of 596 PVs, 576 (96.6%) were isolated using cryoballoons, and 20 required touch-up ablation. PVI was verified during cryoballoon applications with real-time monitoring in 299, and after applications in 280 PVs by Achieve catheters alone. The time-to-isolation was 27.2 ± 22.0 seconds. Validation with standard circumferential mapping catheters confirmed ostial PVIs in 296 of 299 (99.0%) PVs that real-time PVI was obtained during applications, and in 242 of 280 (86.5%) PVs that PV activities were not visible during applications and PVI was verified after the applications. The accuracy of ostial PVIs with Achieve catheters in PVs without obtaining real-time PV recordings was 40/47 (85.1%), 58/65 (89.2%), 77/79 (97.5%), 61/81 (75.3%), and 6/8 (75.0%) in left superior, left inferior, right superior, right inferior, and left common PVs, respectively. In second-generation 28-mm cryoballoon ablation, verification of ostial PVIs using Achieve mapping catheters alone might not be sufficient to accurately confirm an ostial PVI when real-time PVI was not obtained. © 2017 Wiley Periodicals, Inc.

  20. Load Sharing Using Droop Control for Parallel Operation of Matrix Converters as Distributed Generator Interfaces in Isolated Mode

    DEFF Research Database (Denmark)

    Liu, Xiong; Loh, Poh Chiang; Blaabjerg, Frede

    2012-01-01

    or load is connected to the current source side. During grid fault or intentional islanded operation, the DGs need to supply local loads with high quality voltage and share the loads proportionally to their power ratings. This paper proposes droop control strategy for two parallel MCs with two separate......Matrix converter (MC) can be used as ac type distributed generator (DG) interface in microgrid. Reverse power flow operation of MC can be implemented to meet voltage boost requirement, where the input ac generator is connected to the converter's voltage source side and the output utility grid...... be controlled coordinately to make sure that the generator side active power reference is derived from the load side power consumption in a single MC. Simulation results are provided to validate the effectiveness of the control schemes for the proposed parallel MC system....

  1. Effects of inhibiting glutamine synthetase and blocking glutamate uptake on b-wave generation in the isolated rat retina

    OpenAIRE

    Winkler, Barry S.; KAPOUSTA-BRUNEAU, NATALIA; ARNOLD, MATTHEW J.; GREEN, DANIEL G.

    1999-01-01

    The purpose of the present experiments was to evaluate the contribution of the glutamate-glutamine cycle in retinal glial (Müller) cells to photoreceptor cell synaptic transmission. Dark-adapted isolated rat retinas were superfused with oxygenated bicarbonate-buffered media. Recordings were made of the b-wave of the electroretinogram as a measure of light-induced photoreceptor to ON-bipolar neuron transmission. L-methionine sulfoximine (1–10 mM) was added to superfusion media to inhibit gluta...

  2. Electric Power Plants and Generation Stations - POWER_HAZUS_IN: Electric Power Facilities in Indiana, Derived from HAZUS (Federal Emergency Management Agency, Point Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — POWER_HAZUS_IN is a point shapefile that shows locations of electric power facilities in Indiana. POWER_HAZUS_IN was derived from the shapefile named "ELECTRIC." A...

  3. CHARACTERISATION OF BACTERIAL ISOLATES FROM INFECTE D BURN WOUNDS OF PATIENTS ADMITTED IN A TERTIARY LEVEL HEA LTH CARE FACILITY IN NORTHERN REGION OF INDIA

    Directory of Open Access Journals (Sweden)

    Antariksh

    2013-04-01

    Full Text Available ABSTRACT: Infection is an important cause of mortality in bur ns. Emergence of multi drug resistant pathogens in hospital setting has seriously constrained the available therapeutic options. This necessitates periodic review of the iso lation pattern and study of antibiogram of the isolates to strengthen surveillance activities. To determine the bacteriological profile and antimic robial susceptibility pattern of pathogens isolated from infected burn wounds of pati ents admitted in the burns care unit. The present study was carried out over a duration of six months. Pus samples from infected burn wounds were processed following standard protocols. A ntimicrobial susceptibility of the bacterial isolates was performed by Kirby- Bauer dis c diffusion method. A total of 408 bacterial pathogens were isolated from 340 samples. The most fr equent cause of infection was found to be Pseudomonas aeruginosa (53%, followed by Staphyl ococcus aureus (9%, Escherichia coli (9%, Enterobacter spp. (8%, Citrobacter spp. (8%, Kl ebsiella spp. (5%, Acinetobacter spp. (3% and Proteus spp. (3%. High level of drug resist ance (95-100% was observed for cefepime, ceftazidime, amoxyclav, cotrimoxazole and doxycycline among gram negative pathogens. Meropenem, amikacin and ciprofloxacin were found to be most effective. Twenty one percent of the S. aureus isolates were resistant to methicillin. The high prevalence of antimicrobial resistance emphasizes the need for str engthening the infection control practices and regular and periodical surveillance activities t o contain the upward trend of resistance.

  4. Argonne Tape Production Facility systems descriptions. [For generating properly formatted magnetic tapes for Argonne Braille Machine; TAP22, TAP24, TAP25

    Energy Technology Data Exchange (ETDEWEB)

    Foster, R.; Lidinsky, W.

    1977-06-01

    This memorandum describes the operation of the Tape Production Facility. After a brief overview, first the system hardware and then the system software are described. The software consists of two parts: the systems software for the Tape Production Facility and microcode that executes on a SMS Microcontroller. The Microcontroller and some interface hardware implement a dual incremental digital tape transport controller. Logic diagrams and listings for all software are also given. 8 figures.

  5. Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality.

    Science.gov (United States)

    Sampaio, R V; Chiaratti, M R; Santos, D C N; Bressan, F F; Sangalli, J R; Sá, A L A; Silva, T V G; Costa, N N; Cordeiro, M S; Santos, S S D; Ambrosio, C E; Adona, P R; Meirelles, F V; Miranda, M S; Ohashi, O M

    2015-01-15

    Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate. These cells were also subjected to immunophenotypic characterization for the presence of CD90, CD105, and CD79, and the absence of CD45, CD34, and CD73, which are positive and negative markers of MSC, respectively. To prove their multipotency, the cells were induced to differentiate into three different cell types, chondrocytes, osteoblasts, and adipocytes, which were stained with tissue-specific dyes (Chondrogenic-Alcian Blue, Osteogenic-Alizarin Red, and Adipogenic-Oil-Red O, respectively) to confirm differentiation. Gene expression analysis of pluripotency-related genes was also conducted. Our results suggest that adipose tissue from bovines and buffalos can be used as a source of MSC, making adipose tissue-derived cells an interesting option for cell therapy and regenerative medicine. Additionally, these findings have implications for reproductive biotechnology because the use of MSC as nuclear donors has been linked to an increase in the efficiency of nuclear transfer.

  6. [Isolation of a high hydrogen-producing mutant TB34 generated by transposon insertion and analysis of hydrogen production].

    Science.gov (United States)

    Liu, Hong-Yan; Wang, Guang-Ce; Shi, Liu-Yang; Zhu, Da-Ling

    2012-07-01

    To increase the hydrogen-producing capacity of Pantoea agglomerans BH18, isolated from mangrove sludge, we constructed a stable transposon mutagenesis library of this strain. A Tn7-based transposon was randomly inserted into the genomic DNA. Mutants were screened by kanamycin resistance and identified by amplification of the inserted transposon sequences. A mutant strain TB34 was isolated, whose hydrogen production capacity was significantly improved compared to the wild type strain. In seawater-containing medium supplemented with 10 g x L(-1) glucose and had an initial pH of 7.0, the hydrogen yield (H2/glucose) of the mutant strain was (2.04 +/- 0.04) mol x mol(-1), which was 43% higher than that of the wild type strain. The mutant TB34 showed steady hydrogen production capacity for five consecutive passages. Different carbon sources were tested in the hydrogen production by the mutant TB34 and the results showed that both the mutant strain TB34 and the wild type strain BH18 were able to produce hydrogen on sucrose, glucose and fructose. However, different from the wild type strain, the mutant strain TB34 was also able to produce hydrogen using xylose as substrate, with a hydrogen yield (H2/xylose) of (1.34 +/- 0.09) mol x mol(-1), indicating a broader substrate spectrum in the mutant.

  7. Generating Isolated Terawatt-Attosecond X-ray Pulses via a Chirped Laser Enhanced High-Gain Free-electron Laser

    CERN Document Server

    Wang, Zhen; Zhao, Zhentang

    2016-01-01

    A feasible method is proposed to generate isolated attosecond terawatt x-ray radiation pulses in high-gain free-electron lasers. In the proposed scheme, a frequency chirped laser pulse is employed to generate a gradually-varied spacing current enhancement of the electron beam and a series of spatiotemporal shifters are applied between the undulator sections to amplify a chosen ultra-short radiation pulse from self-amplified spontaneous emission. Three-dimensional start-to-end simulations have been carried out and the calculation results demonstrated that 0.15 nm x-ray pulses with peak power over 1TW and duration of several tens of attoseconds could be achieved by using the proposed technique.

  8. Power generation in an isolated community in the state of Tocantins, Brazil; Geracao de energia em uma comunidade isolada no estado de Tocantins

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Weder Ferreira dos; Zukowski Junior, Joel Carlos [Universidade Federal do Tocantins (UFT), Palmas, TO (Brazil); Sodre, Layanni Ferreira [Centro Universitario Luterano de Palmas (CEULP/ULBRA), Palmas, TO (Brazil)], E-mails: weder@gmail.com., zukajr@gmail.com.br, Layanni_sd@hotmail.com

    2011-07-01

    This paper aims to show a case study of the provision of electricity to an isolated community in the region of Jalapao Good Hope community, municipality of Mateiros, State of Tocantins. It was installed a hybrid wind-photovoltaic system with capacity to generate 9676.80 kWh annually. This energy is intended to improve the production process, for that was installed in the community workshop. Moreover, providing space outside of working hours for social community in the community workshop shed coma availability of firm energy during 24h/day. The results showed that the system can be sustainable provided that the community enjoys the potential to generate energy for the production process. (author)

  9. Generating isolated terawatt-attosecond x-ray pulses via a chirped-laser-enhanced high-gain free-electron laser

    Directory of Open Access Journals (Sweden)

    Zhen Wang

    2017-04-01

    Full Text Available A feasible method is proposed to generate isolated attosecond terawatt x-ray radiation pulses in high-gain free-electron lasers. In the proposed scheme, a frequency chirped laser pulse is employed to generate a gradually varied spacing current enhancement of the electron beam, and a series of spatiotemporal shifters are applied between the undulator sections to amplify a chosen ultrashort radiation pulse from self-amplified spontaneous emission. Three-dimensional start-to-end simulations have been carried out, and the calculation results demonstrated that 0.15 nm x-ray pulses with a peak power over 1 TW and a duration of several tens of attoseconds could be achieved by using the proposed technique.

  10. Estimating the Size of the Renewable Energy Generators in an Isolated Solar-Biodiesel Microgrid with Lead-Acid Battery Storage

    Directory of Open Access Journals (Sweden)

    GRAMA Alin

    2015-10-01

    Full Text Available Climate change, fossil fuel decline, expensive power grid extensions focused the attention of scientist in developing electrical power systems that use as primary resources renewable energy generators. Romania has a high renewable energy potential and presents interest in developing renewable energy microgrids using: solar energy, wind energy, biomass Hydro, etc. The paper presents a method of estimating the size of the renewable energy generators in an isolated solar-biodiesel microgrid with lead-acid battery storage. The mathematical model is first presented and then an algorithm is developed to give an estimation of the size of the microgrid. The microgrid is installed in the region of Oradea, Romania. The results are validated through comparison with existing sizing software programs like: PV*Sol and PVSyst.

  11. METC Combustion Research Facility

    Energy Technology Data Exchange (ETDEWEB)

    Halow, J.S.; Maloney, D.J.; Richards, G.A.

    1993-11-01

    The objective of the Morgantown Energy Technology Center (METC) high pressure combustion facility is to provide a mid-scale facility for combustion and cleanup research to support DOE`s advanced gas turbine, pressurized, fluidized-bed combustion, and hot gas cleanup programs. The facility is intended to fill a gap between lab scale facilities typical of universities and large scale combustion/turbine test facilities typical of turbine manufacturers. The facility is now available to industry and university partners through cooperative programs with METC. High pressure combustion research is also important to other DOE programs. Integrated gasification combined cycle (IGCC) systems and second-generation, pressurized, fluidized-bed combustion (PFBC) systems use gas turbines/electric generators as primary power generators. The turbine combustors play an important role in achieving high efficiency and low emissions in these novel systems. These systems use a coal-derived fuel gas as fuel for the turbine combustor. The METC facility is designed to support coal fuel gas-fired combustors as well as the natural gas fired combustor used in the advanced turbine program.

  12. ANTIFUNGAL ACTIVITY OF VOLATILE COMPONENTS GENERATED BY ESSENTIAL OILS AGAINST THE GENUS PENICILLIUM ISOLATED FROM BAKERY PRODUCTS

    Directory of Open Access Journals (Sweden)

    Miroslava Císarová

    2015-02-01

    Full Text Available The aim of this study was evaluation of the antifungal activity of 5 essential oils (EOs. We concretely used thyme, clove, basil, jasmine and rosemary EOs by vapor contact against the fungal species, namely Penicillium citrinum, P. chrysogenum, P. hordei, P. citreonigrum, and P. viridicatum and their ability to affect production of mycotoxins. Each fungus was inoculated in the centre on Czapek Yeast Autolysate Agar (CYA dishes. Dishes were tightly sealed with parafilm and incubated for fourteen days at 25 ± 1 °C (three replicates were used for each treatment. Volatile phase effect of 50 μl of the essential oils was found to inhibit on growth of Penicillium spp.. Complete growth inhibition of the isolates by EOs of thyme and clove was observed. The EO of basil had antifungal effect on growth of P. citreonigrum only after 3rd and 7th day of the incubation at concentration 100 % of EO, like a P. viridicatum, which was inhibited by basil EO (100 % in comparison with control sets. Data was evaluated statistically by 95.0 % Tukey HSD test. In this study we also tested potentional effect of EOs to affect production of mycotoxins of tested Penicillium isolates which are potential toxigenic fungi. After 14 days of incubation with EOs (100 % with control sets, they were screened for a production of mycotoxins by TLC chromatography. Results showed non affecting production of mycotoxins by tested EOs. Conclusions indicate that volatile phase of combinations of thyme oil and clove oil showed good potential in the inhibition of growth of Penicillium spp. EOs should find a practical application in the inhibition of the fungal mycelial growth in some kind of the food.

  13. Carbocations generated under stable conditions by ionization of matrix-Isolated radicals: the allyl and benzyl cations

    OpenAIRE

    Mišić, Vladimir; Piech, Krzysztof; Bally, Thomas

    2013-01-01

    Carbocations are crucial intermediates in many chemical reactions; hence, considerable effort has gone into investigating their structures and properties, for example, in superacids, in salts, or in the gas phase. However, studies of the vibrational structure of carbocations are not abundant, because their infrared spectra are difficult to obtain in superacids or salts (where furthermore the cations may be perturbed by counterions), and the generation of gas-phase carbocations in discharges u...

  14. Blastocystis Isolates from Patients with Irritable Bowel Syndrome and from Asymptomatic Carriers Exhibit Similar Parasitological Loads, but Significantly Different Generation Times and Genetic Variability across Multiple Subtypes.

    Science.gov (United States)

    Vargas-Sanchez, Gie-Bele; Romero-Valdovinos, Mirza; Ramirez-Guerrero, Celedonio; Vargas-Hernandez, Ines; Ramirez-Miranda, Maria Elena; Martinez-Ocaña, Joel; Valadez, Alicia; Ximenez, Cecilia; Lopez-Escamilla, Eduardo; Hernandez-Campos, Maria Elena; Villalobos, Guiehdani; Martinez-Hernandez, Fernando; Maravilla, Pablo

    2015-01-01

    Blastocystis spp is a common intestinal parasite of humans and animals that has been associated to the etiology of irritable bowel syndrome (IBS); however, some studies have not found this association. Furthermore, many biological features of Blastocystis are little known. The objective of present study was to assess the generation times of Blastocystis cultures, from IBS patients and from asymptomatic carriers. A total of 100 isolates were obtained from 50 IBS patients and from 50 asymptomatic carriers. Up to 50 mg of feces from each participant were cultured in Barret's and in Pavlova's media during 48 h. Initial and final parasitological load were measured by microscopy and by quantitative PCR. Amplicons were purified, sequenced and submitted to GenBank; sequences were analysed for genetic diversity and a Bayesian inference allowed identifying genetic subtypes (ST). Generation times for Blastocystis isolates in both media, based on microscopic measures and molecular assays, were calculated. The clinical symptoms of IBS patients and distribution of Blastocystis ST 1, 2 and 3 in both groups was comparable to previous reports. Interestingly, the group of cases showed scarce mean nucleotide diversity (π) as compared to the control group (0.011±0.016 and 0.118±0.177, respectively), whilst high gene flow and small genetic differentiation indexes between different ST were found. Besides, Tajima's D test showed negative values for ST1-ST3. No statistical differences regarding parasitological load between cases and controls in both media, as searched by microscopy and by qPCR, were detected except that parasites grew faster in Barret's than in Pavlova's medium. Interestingly, slow growth of isolates recovered from cases in comparison to those of controls was observed (pBlastocystis might be easily affected by intestinal environmental changes due to IBS probably because virulent strains with slow growth may be selected, reducing their genetic variability.

  15. A3 Altitude Test Facility

    Science.gov (United States)

    Dulreix, Lionel J.

    2009-01-01

    This slide presentation shows drawings, diagrams and photographs of the A3 Altitude Test Facility. It includes a review of the A3 Facility requirements, and drawings of the various sections of the facility including Engine Deck and Superstructure, Test Cell and Thrust Takeout, Structure and Altitude Support Systems, Chemical Steam generators, and the subscale diffuser. There are also pictures of the construction site, and the facility under construction. A Diagram of the A3 Steam system schematic is also shown

  16. Fabrication Facilities

    Data.gov (United States)

    Federal Laboratory Consortium — The Fabrication Facilities are a direct result of years of testing support. Through years of experience, the three fabrication facilities (Fort Hood, Fort Lewis, and...

  17. Second harmonic generation from corona-poled polymer thin films of Y-shape chromophore with different isolation groups

    Indian Academy of Sciences (India)

    Mukesh P Joshi; S Raj Mohan; Balakrishna Kolli; Sarada P Mishra; Akshaya K Palai; Tapan Kanai; T S Dhami; L M Kukreja; A B Samui

    2014-02-01

    We characterize thermal stability of second harmonic generation (SHG) properties of four different Y-type polymers poled using corona poling method. These polymers are based on donor–acceptor–donor-type repeating unit with different aromatic moieties acting as donors and dicyanomethylene acting as an acceptor through conjugated bridge. The donor varies from different substituted benzene to phenothiazine. Polymer containing phenothiazine as donor showed higher SHG intensity and high temperature stability due to rigid repeating unit of phenothiazine compared to others with benzene in the main chain.

  18. A simplified model for generating sequences of global solar radiation data for isolated sites: Using artificial neural network and a library of Markov transition matrices approach

    Energy Technology Data Exchange (ETDEWEB)

    Mellit, A. [University Center of Medea, Institute of Engineering Sciences, Department of Electronics, Ain Dahab, Mdea 26000 (Algeria); Benghanem, M. [University of Sciences Technology Houari Boumediene (USTHB), Faculty of Electrical Engineering, El-Alia, P.O. Box 32, Algiers 16111 (Algeria); Arab, A. Hadj [Development Center of Renewable Energy (CDER), P.O. Box 62, Bouzareah, Algiers 16000 (Algeria); Guessoum, A. [Faculty of Science Engineering, Department of Electronics, Blida University, Blida (Algeria)

    2005-11-01

    The purpose of this work is to develop a hybrid model which will be used to predict the daily global solar radiation data by combining between an artificial neural network (ANN) and a library of Markov transition matrices (MTM) approach. Developed model can generate a sequence of global solar radiation data using a minimum of input data (latitude, longitude and altitude), especially in isolated sites. A data base of daily global solar radiation data has been collected from 60 meteorological stations in Algeria during 1991-2000. Also a typical meteorological year (TMY) has been built from this database. Firstly, a neural network block has been trained based on 60 known monthly solar radiation data from the TMY. In this way, the network was trained to accept and even handle a number of unusual cases. The neural network can generate the monthly solar radiation data. Secondly, these data have been divided by corresponding extraterrestrial value in order to obtain the monthly clearness index values. Based on these monthly clearness indexes and using a library of MTM block we can generate the sequences of daily clearness indexes. Known data were subsequently used to investigate the accuracy of the prediction. Furthermore, the unknown validation data set produced very accurate prediction; with an RMSE error not exceeding 8% between the measured and predicted data. A correlation coefficient ranging from 90% and 92% have been obtained; also this model has been compared to the traditional models AR, ARMA, Markov chain, MTM and measured data. Results obtained indicate that the proposed model can successfully be used for the estimation of the daily solar radiation data for any locations in Algeria by using as input the altitude, the longitude, and the latitude. Also, the model can be generalized for any location in the world. An application of sizing PV systems in isolated sites has been applied in order to confirm the validity of this model. (author)

  19. Next generation sequencing of microRNAs from isogenic neuroblastoma cell lines isolated before and after treatment.

    Science.gov (United States)

    Roth, Sarah Andrea; Knutsen, Erik; Fiskaa, Tonje; Utnes, Peter; Bhavsar, Swapnil; Hald, Øyvind H; Løkke, Cecilie; Mestdagh, Pieter; Johansen, Steinar D; Flægstad, Trond; Einvik, Christer

    2016-03-01

    Neuroblastoma is a pediatric cancer of the developing sympathetic nervous system. High risk neuroblastoma patients typically undergo an initial remission in response to treatment, followed by recurrence of aggressive tumors that have become refractory to further treatment. Recent works have underlined the involvement of microRNAs (miRNAs) in neuroblastoma development and evolution of drug resistance. In this study we have used deep sequencing technology to identify miRNAs differentially expressed in neuroblastoma cell lines isolated from 6 patients at diagnosis and at relapse after intensive treatments. This approach revealed a panel of 42 differentially expressed miRNAs, 8 of which were upregulated and 34 were downregulated. Most strikingly, the 14q32 miRNA clusters encode 22 of the downregulated miRNAs. Reduced expression of 14q32 miRNAs in tumors associated with poor prognosis factors was confirmed in a cohort consisting of 226 primary neuroblastomas. In order to gain insight into the nature of the genes that may be affected by the differentially expressed miRNAs we utilized Ingenuity Pathway Analysis (IPA). This analysis revealed several biological functions and canonical pathways associated with cancer progression and drug resistance. The results of this study contribute to the identification of miRNAs involved in the complex processes of surviving therapeutic treatment and developing drug resistance in neuroblastoma.

  20. The Effect of Herbicides on Hydrogen Peroxide Generation in Isolated Vacuoles of Red Beet Root (Beta vulgaris L.

    Directory of Open Access Journals (Sweden)

    E.V. Pradedova

    2015-12-01

    Full Text Available Influence of herbicides on the hydrogen peroxide generation in vacuolar extracts of red beet root (Beta vulgaris L. was investigated. Belonging to different chemical classes of herbicide compounds have been used. Herbicides differ from each other in the mechanism of effects on plants. Clopyralid (aromatic acid herbicide, derivative of picolinic acid and 2.4-D (phenoxyacetic herbicide, characterized by hormone-like effects, contributed to the formation of H2O2 in vacuolar extracts. Fluorodifen (nitrophenyl ether herbicide and diuron (urea herbicide also have increased contents H2O2. These compounds inhibit the electron transport, photosynthesis, and photorespiration in sensitive plants. Herbicidal effect of glyphosate (organophosphorus herbicide is due to the inhibition of amino acid synthesis in plant cells. Glyphosate did not affect the content of H2O2 in vacuolar extracts. Herbicide dependent H2O2-generation did not occur with oxidoreductase inhibitors, potassium cyanide and sodium azide. The results suggest that the formation of ROS in the vacuoles due to activity of oxidoreductases, which could interact with herbicides.

  1. Biomass gasification systems in electric energy generation for isolated communities; Sistemas de gaseificacao de biomassa na geracao de energia eletrica para comunidades isoladas

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira; Velazquez, Silvia M. Stortine Gonzales; Martins, Osvaldo Stella; Santos, Sandra Maria Apolinario dos; Basaglia, Fernando [Centro Nacional de Referencia em Biomassa (CENBIO), Sao Paulo, SP (Brazil)], e-mail: suani@iee.usp.br, e-mail: sgvelaz@iee.usp.br, e-mail: omartins@iee.usp.br, e-mail: sandra@iee.usp.br, e-mail: basaglia@iee.usp.br; Ushima, Ademar Hakuo [Instituto de Pesquisas Tecnologicas (IPT), Sao Paulo, SP (Brazil)], e-mail: adidas@ipt.br

    2004-07-01

    The project 'Comparison Among Existing Technologies of Biomass Gasification', agreement FINEP/CT-ENERG 23.01.0695.00, is a partnership between CENBIO - The Brazilian Reference Center on Biomass, BUN - Biomass Users Network of Brazil, IPT - Technology Research Institute and UA - Amazon University. The main objective of this project is to study a biomass gasifier system and its implantation, using a sustainable way, at isolated communities in the North Region, offering an alternative to replace fossil fuel. The system is composed by a gasifier from Indian Institute of Science - IISc, that can generate 20 kW of output energy, a generator (internal combustion engine), an ashes extractor, a water cooler and treatment system, a dryer and a control panel. The project, developed at IPT, intends to evaluate the operation conditions of the gasification system: gas cleaning, electric power generation and the technology transfer to Brazil, allowing the formation of human resources in the Brazilian North region and collaborating with the national institutions from this area. (author)

  2. Generation of free radicals from organic hydroperoxide tumor promoters in isolated mouse keratinocytes. Formation of alkyl and alkoxyl radicals from tert-butyl hydroperoxide and cumene hydroperoxide.

    Science.gov (United States)

    Taffe, B G; Takahashi, N; Kensler, T W; Mason, R P

    1987-09-05

    The organic hydroperoxides tert-butyl hydroperoxide and cumene hydroperoxide are tumor promoters in the skin of SENCAR mice, and this activity is presumed to be mediated through the activation of the hydroperoxides to free radical species. In this study we have assessed the generation of free radicals from organic hydroperoxides in the target cell (the murine basal keratinocyte) using electron spin resonance. Incubation of primary isolates of keratinocytes from SENCAR mice in the presence of spin traps (5,5-dimethyl-1-pyrroline N-oxide or 2-methyl-2-nitrosopropane) and either tert-butyl hydroperoxide or cumene hydroperoxide resulted in the generation and detection of radical adducts of these spin traps. tert-Butyl alkoxyl and alkyl radical adducts of 5,5-dimethyl-1-pyrroline N-oxide were detected shortly after addition of tert-butyl hydroperoxide, whereas only alkyl radical adducts were observed with cumene hydroperoxide. Spin trapping of the alkyl radicals with 2-methyl-2-nitrosopropane led to the identification of methyl and ethyl radical adducts following both tert-butyl hydroperoxide and cumene hydroperoxide exposures. Prior heating of the cells to 100 degrees C for 30 min prevented radical formation. The radical generating capacity of subcellular fractions of these epidermal cells was examined using 5,5-dimethyl-1-pyrroline N-oxide and cumene hydroperoxide, and this activity was confined to the 105,000 X g supernatant fraction.

  3. Wind Energy Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Laurie, Carol

    2017-02-01

    This book takes readers inside the places where daily discoveries shape the next generation of wind power systems. Energy Department laboratory facilities span the United States and offer wind research capabilities to meet industry needs. The facilities described in this book make it possible for industry players to increase reliability, improve efficiency, and reduce the cost of wind energy -- one discovery at a time. Whether you require blade testing or resource characterization, grid integration or high-performance computing, Department of Energy laboratory facilities offer a variety of capabilities to meet your wind research needs.

  4. Wind Energy Facilities

    Energy Technology Data Exchange (ETDEWEB)

    Office of Energy Efficiency and Renewable Energy

    2017-02-01

    This book takes readers inside the places where daily discoveries shape the next generation of wind power systems. Energy Department laboratory facilities span the United States and offer wind research capabilities to meet industry needs. The facilities described in this book make it possible for industry players to increase reliability, improve efficiency, and reduce the cost of wind energy -- one discovery at a time. Whether you require blade testing or resource characterization, grid integration or high-performance computing, Department of Energy laboratory facilities offer a variety of capabilities to meet your wind research needs.

  5. Metabolic and growth characteristics of novel diverse microbes isolated from deep cores collected at the Next Generation Ecosystem Experiment (NGEE)-Arctic site in Barrow, Alaska

    Science.gov (United States)

    Chakraborty, R.; Pettenato, A.; Tas, N.; Hubbard, S. S.; Jansson, J.

    2013-12-01

    The Arctic is characterized by vast amounts of carbon stored in permafrost and is an important focal point for the study of climate change as increasing temperature may accelerate microbially mediated release of Carbon stored in permafrost into the atmosphere as CO2 and CH4. Yet surprisingly, very little is known about the vulnerability of permafrost and response of microorganisms in the permafrost to their changing environment. This deficiency is largely due to the difficulty in study of largely uncultivated and unknown permafrost microbes. As part of the U.S. Department of Energy (DOE) Next Generation Ecosystem Experiment (NGEE) in the Arctic, we collected permafrost cores in an effort to isolate resident microbes. The cores were from the Barrow Environmental Observatory (BEO), located at the northern most location on the Alaskan Arctic Coastal Plain near Barrow, AK, and up to 3m in depth. In this location, permafrost starts from 0.5m in depth and is characterized by variable water content and higher pH than surface soils. Enrichments for heterotrophic bacteria were initiated at 4°C and 1°C in the dark in several different media types, under both aerobic and anaerobic conditions. Positive enrichments were identified by an increase in optical density and cell counts after incubation period ranging from two to four weeks. After serial transfers into fresh media, individual colonies were obtained on agar surface. Several strains were isolated that include Firmicutes such as Bacillus, Clostridium, Sporosarcina, and Paenibacillus species and Iron-reducing Betaproteobacteria such as Rhodoferax species. In addition, methanogenic enrichments continue to grow and produce methane gas at 2°C. In this study, we present the characterization, carbon substrate utilization, pH, temperature and osmotic tolerance, as well as the effect of increasing climate change parameters on the growth rate and respiratory gas production from these permafrost isolates.

  6. Molecular and Biological Characterization of an Isolate of Cucumber mosaic virus from Glycine soja by Generating its Infectious Full-genome cDNA Clones

    Directory of Open Access Journals (Sweden)

    Mi Sa Vo Phan

    2014-06-01

    Full Text Available Molecular and biological characteristics of an isolate of Cucumber mosaic virus (CMV from Glycine soja (wild soybean, named as CMV-209, was examined in this study. Comparison of nucleotide sequences and phylogenetic analyses of CMV-209 with the other CMV strains revealed that CMV-209 belonged to CMV subgroup I. However, CMV-209 showed some genetic distance from the CMV strains assigned to subgroup IA or subgroup IB. Infectious full-genome cDNA clones of CMV-209 were generated under the control of the Cauliflower mosaic virus 35S promoter. Infectivity of the CMV-209 clones was evaluated in Nicotiana benthamiana and various legume species. Our assays revealed that CMV-209 could systemically infect Glycine soja (wild soybean and Pisum sativum (pea as well as N. benthamiana, but not the other legume species.

  7. Generation of hydroxyl radical in isolated pea root cell wall, and the role of cell wall-bound peroxidase, Mn-SOD and phenolics in their production.

    Science.gov (United States)

    Kukavica, Biljana; Mojovic, Milos; Vuccinic, Zeljko; Maksimovic, Vuk; Takahama, Umeo; Jovanovic, Sonja Veljovic

    2009-02-01

    The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H(2)O(2) formation from dioxygen; and (ii) the POD-catalyzed reduction of H(2)O(2) to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (.OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate .OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electropho-resis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between .OH and the superoxide radical (O(2)(-).). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPO/OH adduct formation. A decrease in DEPMPO/OH adduct formation in the presence of H(2)O(2) scavengers demonstrated that this hydroxyl radical was derived from H(2)O(2). During the generation of .OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H(2)O(2) required for the formation of .OH in isolated cell walls is produced during the reduction of O(2) by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce .OH. Addition of exogenous H(2)O(2) again induced the production of .OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions

  8. Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation

    Directory of Open Access Journals (Sweden)

    Wang Wei

    2010-11-01

    Full Text Available Abstract Background Degradation of the toxic compounds generated in the harsh pretreatment of lignocellulose is an inevitable step in reducing the toxin level for conducting practical enzymatic hydrolysis and ethanol fermentation processes. Various detoxification methods have been tried and many negative outcomes were found using these methods, such as the massive freshwater usage and wastewater generation, loss of the fine lignocellulose particles and fermentative sugars and incomplete removal of inhibitors. An alternate method, biodetoxification, which degrades the toxins as part of their normal metabolism, was considered a promising option for the removal of toxins without causing the above problems. Results A kerosene fungus strain, Amorphotheca resinae ZN1, was isolated from the microbial community growing on the pretreated corn stover material. The degradation of the toxins as well as the lignocelluloses-derived sugars was characterized in different ways, and the results show that A. resinae ZN1 utilized each of these toxins and sugars as the sole carbon sources efficiently and grew quickly on the toxins. It was found that the solid-state culture of A. resinae ZN1 on various pretreated lignocellulose feedstocks such as corn stover, wheat straw, rice straw, cotton stalk and rape straw degraded all kinds of toxins quickly and efficiently. The consequent simultaneous saccharification and ethanol fermentation was performed at the 30% (wt/wt solid loading of the detoxified lignocellulosic feedstocks without a sterilization step, and the ethanol titer in the fermentation broth reached above 40 g/L using food crop residues as feedstocks. Conclusions The advantages of the present biodetoxification by A. resinae ZN1 over the known detoxification methods include zero energy input, zero wastewater generation, complete toxin degradation, processing on solid pretreated material, no need for sterilization and a wide lignocellulose feedstock spectrum

  9. 30万t/aPVC装置乙炔站自控仪表改造%Modifications on automatic control instruments of acetylene generating station for 30 000 t/a PVC production facility

    Institute of Scientific and Technical Information of China (English)

    张春严

    2012-01-01

    Modifications were made on the DCS power supply system, automatic control sys- tems for the nitrogen replacement of upper hopper and acetylene generator feeding, and the sodium hypochlorite preparation system in the acetylene generating station for 30 000 t/a PVC production facility. Thus, the production reliability of acetylene generating station was improved, the hidden hazards were eliminated, and the labour intensity was reduced.%对30万t/aPVC装置乙炔站的DCS供电系统、发生器上储斗充氮置换及加料自控系统、次氯酸钠配制系统进行了改造,通过改造提高了乙炔站生产的可靠性,消除了安全隐患,降低了劳动强度。

  10. Hydrogen generation during melter feed preparation of Tank 42 sludge and salt washed loaded CST in the Defense Waste Processing Facility

    Energy Technology Data Exchange (ETDEWEB)

    Daniel, W.E.

    1999-12-08

    The main objective of these scoping tests was to measure the rate of hydrogen generation in a series of experiments designed to duplicate the expected SRAT and SME processing conditions in laboratory scale vessels. This document details the testing performed to determine the maximum hydrogen generation expected with a coupled flowsheet of sludge, loaded CST [crystalline silicotitanate], and frit.

  11. Generation of electric energy in isolated rural communities in the Amazon Region a proposal for the autonomy and sustainability of the local populations

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Celia Salama [Energy and Environment Planning Program, COPPE/UFRJ - Cidade Universitaria, Centro de Tecnologia, Rio de Janeiro - RJ (Brazil); Rosa, Luiz Pinguelli [Energy and Environment Planning Program, COPPE/UFRJ - Director of COPPE/UFRJ - Cidade Universitaria, Centro de Tecnologia, Bloco G, sala 101, Ilha do Fundao, Rio de Janeiro - RJ (Brazil); Silva, Neilton Fidelis da [IVIG/COPPE/UFRJ, CETS, Predio anexo ao Centro de Tecnologia, Rua Pedro Calmon, s/no. Cidade Universitaria, Ilha do Fundao, Rio de Janeiro - RJ (Brazil)

    2011-01-15

    The Brazilian Amazon Region is living with the consequences of governance based on predatory extraction of its natural resources, bringing little or no benefit to the local population. The region suffers from socioeconomic inequality and lack of basic infrastructure. The populations of the Amazon Region, if and when supplied with electricity at all, are provided with a low quality service based on energy generation from fossil fuels, subject to difficulties in terms of access and logistics, and negative environmental impacts. There have been programs aimed at the universalization of electricity services, such as the current Programa Luz para Todos (Light for All Program), but they have not succeeded in altering this scenario in the North region of the country. In this paper we present the result of these initiatives and an overview of the Isolated Systems in the Amazon Region. We propose in this paper that the provision of electric energy be effectively considered part of a plan for generating wealth and employment for the region's inhabitants, whilst preserving the environment. The solutions offered to each community should take into account the need to manage renewable energy sources, should be developed together with local populations, and drawing on the lessons learned from other projects. The specific characteristics and needs of the communities should be taken into account and local people should be empowered as citizens by means of their involvement in the development possibilities. (author)

  12. Calculating the Energy Cost of CO2 Removal in a Coal Based Gas Turbine Fuel Cell Hybrid Power Generation System with an Isolated Anode Stream

    Energy Technology Data Exchange (ETDEWEB)

    Vanosdol, J G; Gemmen, R S; Liese, E A

    2007-10-01

    In recent years there has been significant interest in identifying carbon capturing technologies that can be applied to fossil fuel power generation plants.CO2 capture technologies seek to reduce the amount of CO2 that would normally be emitted into the atmosphere from the daily operation of these plants. In terms of system efficiency and operating costs, this carbon capture is expensive. Further, the additional equipment that would be used to capture CO2 emissions greatly adds to the complexity of the system. There has also been significant interest in coal based gas turbine fuel cell hybrid power plants. A hybrid power plant can have much greater system efficiency than a normal gas turbine power plant because the heat that is normally unused in a standalone solid oxide fuel cell (SOFC) is recovered and used to drive a power producing turbine. It is thought that the increased system efficiency of the hybrid system might compensate for the increased expense of performing carbon capture. In order to provide some analytical insight on this tradeoff we present a 100 MW class coal fired gas turbine SOFC hybrid power generation system. The hybrid system operates at a pressure ratio of 6, and uses heat recuperation and cathode air recirculation to control the SOFC inlet temperature and the temperature change across the SOFC. A carbon capture scheme is added to this system in order to calculate the relative energy cost in terms of system efficiency due to CO2 compression. The carbon capture is performed by burning the unused fuel from the SOFC in an isolated anode stream using pure O2 injection. The resulting heat that is generated from this process is then used to drive a secondary turbine that is placed in the anode exhaust stream where more work is extracted. With an isolated anode stream, the products of combustion from this secondary combustion process are mostly water and carbon dioxide. The water by-product is

  13. Final Report: Detection and Characterization of Underground Facilities by Stochastic Inversion and Modeling of Data from the New Generation of Synthetic Aperture Satellites

    Energy Technology Data Exchange (ETDEWEB)

    Foxall, W; Cunningham, C; Mellors, R; Templeton, D; Dyer, K; White, J

    2012-02-27

    Many clandestine development and production activities can be conducted underground to evade surveillance. The purpose of the study reported here was to develop a technique to detect underground facilities by broad-area search and then to characterize the facilities by inversion of the collected data. This would enable constraints to be placed on the types of activities that would be feasible at each underground site, providing a basis the design of targeted surveillance and analysis for more complete characterization. Excavation of underground cavities causes deformation in the host material and overburden that produces displacements at the ground surface. Such displacements are often measurable by a variety of surveying or geodetic techniques. One measurement technique, Interferometric Synthetic Aperture Radar (InSAR), uses data from satellite-borne (or airborne) synthetic aperture radars (SARs) and so is ideal for detecting and measuring surface displacements in denied access regions. Depending on the radar frequency and the acquisition mode and the surface conditions, displacement maps derived from SAR interferograms can provide millimeter- to centimeter-level measurement accuracy on regional and local scales at spatial resolution of {approx}1-10 m. Relatively low-resolution ({approx}20 m, say) maps covering large regions can be used for broad-area detection, while finer resolutions ({approx}1 m) can be used to image details of displacement fields over targeted small areas. Surface displacements are generally expected to be largest during or a relatively short time after active excavation, but, depending on the material properties, measurable displacement may continue at a decreasing rate for a considerable time after completion. For a given excavated volume in a given geological setting, the amplitude of the surface displacements decreases as the depth of excavation increases, while the area of the discernable displacement pattern increases. Therefore, the

  14. A cryogenic test facility

    Science.gov (United States)

    Veenendaal, Ian

    The next generation, space-borne instruments for far infrared spectroscopy will utilize large diameter, cryogenically cooled telescopes in order to achieve unprecedented sensitivities. Low background, ground-based cryogenic facilities are required for the cryogenic testing of materials, components and subsystems. The Test Facility Cryostat (TFC) at the University of Lethbridge is a large volume, closed cycle, 4K cryogenic facility, developed for this purpose. This thesis discusses the design and performance of the facility and associated external instrumentation. An apparatus for measuring the thermal properties of materials is presented, and measurements of the thermal expansion and conductivity of carbon fibre reinforced polymers (CFRPs) at cryogenic temperatures are reported. Finally, I discuss the progress towards the design and fabrication of a demonstrator cryogenic, far infrared Fourier transform spectrometer.

  15. PREVALENCE AND ANTIMICROBIAL RESISTANCE OF SALMONELLA ISOLATED FROM CARCASSES, PROCESSING FACILITIES AND THE ENVIRONMENT SURROUNDING SMALL SCALE POULTRY SLAUGHTERHOUSES IN THAILAND.

    Science.gov (United States)

    Chotinun, Suwit; Rojanasthien, Suvichai; Unger, Fred; Tadee, Pakpoom; Patchanee, Prapas

    2014-11-01

    Salmonella is a major food-borne pathogen worldwide, including Thai- land, and poultry meat plays a role as a vehicle for the spread of the disease from animals to humans. The prevalence and characteristics of Salmonella isolated from 41 small scale poultry slaughterhouses in Chiang Mai, Thailand were determined during July 2011 through May 2012. Salmonella's prevalence in live poultry, car- casses, waste water, and soil around processing plants were 3.2%, 7.3%, 22.0% and 29.0%, respectively. Eighteen different serotypes were identified, the most common being Corvallis (15.2%), followed by Rissen (13.9%), Hadar (12.7%), Enteritidis (10.1%), [I. 4,5,12:i:-] (8.8%), Stanley (8.8%), and Weltevreden (8.8%). Antimicrobial susceptibility tests revealed that 68.4% of the Salmonella spp were resistant to at least one antimicrobial while 50.6% showed multiple drug resis- tance (MDR). Specifically, 44.3% of Salmonella were resistant to nalidixic acid, followed by streptomycin (41.8%), ampicillin (34.2%), tetracycline (34.2%), and sulfamethoxazole/trimethoprim (20.3%). Salmonella contamination was found in processing lines, carcasses, and in the environment around the processing sta- tions. These findings indicate that improving hygiene management in small scale poultry slaughterhouses as well as prudent use of antimicrobial drugs is urgently needed if Salmonella contamination is to be reduced.

  16. Facile Isolation of Adsorbent-Free Long and Highly-Pure Chirality-Selected Semiconducting Single-Walled Carbon Nanotubes Using A Hydrogen-bonding Supramolecular Polymer

    Science.gov (United States)

    Toshimitsu, Fumiyuki; Nakashima, Naotoshi

    2015-12-01

    The ideal form of semiconducting-single-walled carbon nanotubes (sem-SWNTs) for science and technology is long, defect-free, chirality pure and chemically pure isolated narrow diameter tubes. While various techniques to solubilize and purify sem-SWNTs have been developed, many of them targeted only the chiral- or chemically-purity while sacrificing the sem-SWNT intrinsic structural identities by applying strong ultra-sonication and/or chemical modifications. Toward the ultimate purification of the sem-SWNTs, here we report a mild-conditioned extraction of the sem-SWNTs using removable supramolecular hydrogen-bonding polymers (HBPs) that are composed of dicarboxylic- or diaminopyridyl-fluorenes with ~70%-(8,6)SWNT selective extraction. Replacing conventional strong sonication techniques by a simple shaking using HPBs was found to provide long sem-SWNTs (>2.0 μm) with a very high D/G ratio, which was determined by atomic force microscopy observations. The HBPs were readily removed from the nanotube surfaces by an outer stimulus, such as a change in the solvent polarities, to provide chemically pure (8,6)-enriched sem-SWNTs. We also describe molecular mechanics calculations to propose possible structures for the HBP-wrapped sem-SWNTs, furthermore, the mechanism of the chiral selectivity for the sorted sem-SWNTs is well explained by the relationship between the molecular surface area and mass of the HBP/SWNT composites.

  17. Analysis of accident sequences and source terms at waste treatment and storage facilities for waste generated by U.S. Department of Energy Waste Management Operations, Volume 3: Appendixes C-H

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, C.; Nabelssi, B.; Roglans-Ribas, J. [and others

    1995-04-01

    This report contains the Appendices for the Analysis of Accident Sequences and Source Terms at Waste Treatment and Storage Facilities for Waste Generated by the U.S. Department of Energy Waste Management Operations. The main report documents the methodology, computational framework, and results of facility accident analyses performed as a part of the U.S. Department of Energy (DOE) Waste Management Programmatic Environmental Impact Statement (WM PEIS). The accident sequences potentially important to human health risk are specified, their frequencies are assessed, and the resultant radiological and chemical source terms are evaluated. A personal computer-based computational framework and database have been developed that provide these results as input to the WM PEIS for calculation of human health risk impacts. This report summarizes the accident analyses and aggregates the key results for each of the waste streams. Source terms are estimated and results are presented for each of the major DOE sites and facilities by WM PEIS alternative for each waste stream. The appendices identify the potential atmospheric release of each toxic chemical or radionuclide for each accident scenario studied. They also provide discussion of specific accident analysis data and guidance used or consulted in this report.

  18. Final Report: Detection and Characterization of Underground Facilities by Stochastic Inversion and Modeling of Data from the New Generation of Synthetic Aperture Satellites

    Energy Technology Data Exchange (ETDEWEB)

    Foxall, W; Cunningham, C; Mellors, R; Templeton, D; Dyer, K; White, J

    2012-02-27

    Many clandestine development and production activities can be conducted underground to evade surveillance. The purpose of the study reported here was to develop a technique to detect underground facilities by broad-area search and then to characterize the facilities by inversion of the collected data. This would enable constraints to be placed on the types of activities that would be feasible at each underground site, providing a basis the design of targeted surveillance and analysis for more complete characterization. Excavation of underground cavities causes deformation in the host material and overburden that produces displacements at the ground surface. Such displacements are often measurable by a variety of surveying or geodetic techniques. One measurement technique, Interferometric Synthetic Aperture Radar (InSAR), uses data from satellite-borne (or airborne) synthetic aperture radars (SARs) and so is ideal for detecting and measuring surface displacements in denied access regions. Depending on the radar frequency and the acquisition mode and the surface conditions, displacement maps derived from SAR interferograms can provide millimeter- to centimeter-level measurement accuracy on regional and local scales at spatial resolution of {approx}1-10 m. Relatively low-resolution ({approx}20 m, say) maps covering large regions can be used for broad-area detection, while finer resolutions ({approx}1 m) can be used to image details of displacement fields over targeted small areas. Surface displacements are generally expected to be largest during or a relatively short time after active excavation, but, depending on the material properties, measurable displacement may continue at a decreasing rate for a considerable time after completion. For a given excavated volume in a given geological setting, the amplitude of the surface displacements decreases as the depth of excavation increases, while the area of the discernable displacement pattern increases. Therefore, the

  19. By-law from July 4, 2003, relative to the technical prescriptions of design and operation for the connection of a power generating facility to the public power transportation grid; Arrete du 4 juillet 2003 relatif aux prescriptions techniques de conception et de fonctionnement pour le raccordement au reseau public de transport d'une installation de production d'energie electrique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-09-01

    This by-law fixes the constructive and organisational dispositions that must fulfill the power generating facilities in order to make their connection to the public power grid conformable with the objectives of the decree from June 27, 2003. The dispositions concern: the voltage, connection scheme, protection systems, earthing, domain of operation, frequency adjustment and quality of current, communication and remote control systems of the power generating facility. (J.S.)

  20. Cell-type-specific profiling of protein-DNA interactions without cell isolation using targeted DamID with next-generation sequencing.

    Science.gov (United States)

    Marshall, Owen J; Southall, Tony D; Cheetham, Seth W; Brand, Andrea H

    2016-09-01

    This protocol is an extension to: Nat. Protoc. 2, 1467-1478 (2007); doi:10.1038/nprot.2007.148; published online 7 June 2007The ability to profile transcription and chromatin binding in a cell-type-specific manner is a powerful aid to understanding cell-fate specification and cellular function in multicellular organisms. We recently developed targeted DamID (TaDa) to enable genome-wide, cell-type-specific profiling of DNA- and chromatin-binding proteins in vivo without cell isolation. As a protocol extension, this article describes substantial modifications to an existing protocol, and it offers additional applications. TaDa builds upon DamID, a technique for detecting genome-wide DNA-binding profiles of proteins, by coupling it with the GAL4 system in Drosophila to enable both temporal and spatial resolution. TaDa ensures that Dam-fusion proteins are expressed at very low levels, thus avoiding toxicity and potential artifacts from overexpression. The modifications to the core DamID technique presented here also increase the speed of sample processing and throughput, and adapt the method to next-generation sequencing technology. TaDa is robust, reproducible and highly sensitive. Compared with other methods for cell-type-specific profiling, the technique requires no cell-sorting, cross-linking or antisera, and binding profiles can be generated from as few as 10,000 total induced cells. By profiling the genome-wide binding of RNA polymerase II (Pol II), TaDa can also identify transcribed genes in a cell-type-specific manner. Here we describe a detailed protocol for carrying out TaDa experiments and preparing the material for next-generation sequencing. Although we developed TaDa in Drosophila, it should be easily adapted to other organisms with an inducible expression system. Once transgenic animals are obtained, the entire experimental procedure-from collecting tissue samples to generating sequencing libraries-can be accomplished within 5 d.

  1. Orientation to pollution prevention for facility design

    Energy Technology Data Exchange (ETDEWEB)

    Raney, E.A.; Whitehead, J.K.; Encke, D.B. [Westinghouse Hanford Co., Richland, WA (United States); Dorsey, J.A. [Kaiser Engineers Hanford Co., Richland, WA (United States)

    1994-01-01

    This material was developed to assist engineers in incorporating pollution prevention into the design of new or modified facilities within the U.S. Department of Energy (DOE). The material demonstrates how the design of a facility can affect the generation of waste throughout a facility`s entire life and it offers guidance on how to prevent the generation of waste during design. Contents include: Orientation to pollution prevention for facility design training course booklet; Pollution prevention design guideline; Orientation to pollution prevention for facility design lesson plan; Training participant survey and pretest; and Training facilitator`s guide and schedule.

  2. Accelerated radioactive nuclear beams: Existing and planned facilities

    Energy Technology Data Exchange (ETDEWEB)

    Nitschke, J.M.

    1992-07-01

    An over-view of existing and planned radioactive nuclear beam facilities world-wide. Two types of production methods are distinguished: projectile fragmentation and the on-line isotope separator (ISOL) method. While most of the projectile fragmentation facilities are already in operation, almost all the ISOL-based facilities are in still the planning stage.

  3. Mammography Facilities

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Mammography Facility Database is updated periodically based on information received from the four FDA-approved accreditation bodies: the American College of...

  4. Health Facilities

    Science.gov (United States)

    Health facilities are places that provide health care. They include hospitals, clinics, outpatient care centers, and specialized care centers, such as birthing centers and psychiatric care centers. When you ...

  5. Canyon Facilities

    Data.gov (United States)

    Federal Laboratory Consortium — B Plant, T Plant, U Plant, PUREX, and REDOX (see their links) are the five facilities at Hanford where the original objective was plutonium removal from the uranium...

  6. Isolation and characterization of microsatellite markers in Garcinia gummi-gutta by next-generation sequencing and cross-species amplification

    Indian Academy of Sciences (India)

    K. V. RAVISHANKAR; R. VASUDEVA; BYATROY HEMANTH; B. S. SANDYA; B. R. STHAPIT; V. A. PARTHASARATHY; V. RAMANATHA RAO

    2017-06-01

    Garcinia gummi-gutta (L.) Roxb. (Clusiaceae) is an endemic, semidomesticated, fruit-yielding tree species distributed in the Western Ghats of India and Sri Lanka. Various bioactive phytochemicals, such as garcinol, benzophenones and xanthones are isolated from G. gummi-gutta and have shown antibacterial, antiviral and antioxidant activities. We sequenced the totalgenomic DNA using Illumina Hiseq 2000 platform and examined 241,141,804 bp high quality data, assembled into 773,889 contigs. In these contigs, 27,313 simple-sequence repeats (SSRs) were identified, among which mononucleotide repeats were predominant (44.98%) followed by dinucleotide and trinucleotide repeats. Primers were designed for 9964 microsatellites among which 32 randomly selected SSR primer pairs were standardized for amplification. Polymerase chain reaction (PCR) amplification of genomic DNA in 30 G. gummi-gutta genotypes revealed polymorphic information content (PIC) across all 32 loci ranging from 0.867 to 0.951, with a mean value of 0.917. The observed and expected heterozygosity ranged from 0.00 to 0.63 and 0.896 to 0.974, respectively. Alleles per locus ranged from 12 to 27. This is the first report on the development of genomic SSR markers in G. gummi-gutta using next-generation sequencing technology. The genomic SSR markers developedin this study will be useful in identification, mapping, diversity and breeding studies.

  7. Reverse Ballistic Air Gun Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This custom-designed facility houses a suite of three air guns capable of generating accelerations up to 100,000 Gs and velocities up to 2,000 ft/s. In addition to a...

  8. Reverse Ballistic Air Gun Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This custom-designed facility houses a suite of three air guns capable of generating accelerations up to 100,000 Gs and velocities up to 2,000 ft/s. In addition to...

  9. Waste Isolation Pilot Plant Transuranic Waste Baseline inventory report. Volume 2. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-02-01

    This document is the Baseline Inventory Report for the transuranic (alpha-bearing) wastes stored at the Waste Isolation Pilot Plant (WIPP) in New Mexico. Waste stream profiles including origin, applicable EPA codes, typical isotopic composition, typical waste densities, and typical rates of waste generation for each facility are presented for wastes stored at the WIPP.

  10. The Generic Data Capture Facility

    Science.gov (United States)

    Connell, Edward B.; Barnes, William P.; Stallings, William H.

    The Generic Data Capture Facility, which can provide data capture support for a variety of different types of spacecraft while enabling operations costs to be carefully controlled, is discussed. The data capture functions, data protection, isolation of users from data acquisition problems, data reconstruction, and quality and accounting are addressed. The TDM and packet data formats utilized by the system are described, and the development of generic facilities is considered.

  11. Facile synthesis of near-monodisperse Ag@Ni core-shell nanoparticles and their application for catalytic generation of hydrogen.

    Science.gov (United States)

    Guo, Huizhang; Chen, Yuanzhi; Chen, Xiaozhen; Wen, Ruitao; Yue, Guang-Hui; Peng, Dong-Liang

    2011-05-13

    Magnetically recyclable Ag-Ni core-shell nanoparticles have been fabricated via a simple one-pot synthetic route using oleylamine both as solvent and reducing agent and triphenylphosphine as a surfactant. As characterized by transmission electron microscopy (TEM), the as-synthesized Ag-Ni core-shell nanoparticles exhibit a very narrow size distribution with a typical size of 14.9 ± 1.2 nm and a tunable shell thickness. UV-vis absorption spectroscopy study shows that the formation of a Ni shell on Ag core can damp the surface plasmon resonance (SPR) of the Ag core and lead to a red-shifted SPR absorption peak. Magnetic measurement indicates that all the as-synthesized Ag-Ni core-shell nanoparticles are superparamagnetic at room temperature, and their blocking temperatures can be controlled by modulating the shell thickness. The as-synthesized Ag-Ni core-shell nanoparticles exhibit excellent catalytic properties for the generation of H(2) from dehydrogenation of sodium borohydride in aqueous solutions. The hydrogen generation rate of Ag-Ni core-shell nanoparticles is found to be much higher than that of Ag and Ni nanoparticles of a similar size, and the calculated activation energy for hydrogen generation is lower than that of many bimetallic catalysts. The strategy employed here can also be extended to other noble-magnetic metal systems.

  12. Diagnosis methodology applied to the preventive maintenance of power generation units in isolated sites; Methodologie de diagnostic appliquee a la maintenance preventive d'unites de production d'electricite en sites isoles

    Energy Technology Data Exchange (ETDEWEB)

    Marie-Joseph, M.

    2003-04-01

    Todays, overseas territories gather more than 25% of the photovoltaic systems of the French territory. More than 600 kWc of this photovoltaic park is located in French Guiana and 75% in isolated areas. This leads to the problem of implementation of a convenient maintenance policy mainly because of the problem of supply of replacement parts, of the lack of qualified local manpower, and of the complexity of a fast diagnosis of the installations. Therefore, the development of a diagnosis tool for the different kinds of failures and defects that can occur on a photovoltaic power system is of prime importance. Starting from the elements of preventive maintenance philosophy developed in the thesis of S. Salvat, this work describes the development of a functional simulation tool allowing the validation of these first results. Using models for the simulation of each sub-part of the system, involving a statistical modeling of the solar resource and of the connected load, a detailed analysis of the operation defects is presented. This tool, which is a key element of the elaboration of the diagnosis, allows the use of detection methods based on pattern recognition principle. The supervised learning makes a classification using the search for a parametric discriminating function minimizing the quadratic error on the base. The algorithms performing the real-time monitoring of the system in the presence of defects are fitted in an experimental kit representing the production of an individual generator at a reduced scale. The experimental detection procedures are then tested on production and regulation defects and then compared to those obtained from simulations performed in the same operating conditions. (J.S.)

  13. Participation in sizing methods and management improvement for the isolated hybrid systems coupling photovoltaic generator and power generating unit. Application in developing countries; Participation a l'amelioration des methodes de dimensionnement et de gestion pour les systemes isoles hybrides de production d'electricite, couplant generateur photovoltaique et groupe electrogene. Application dans les pays en voie de developpement

    Energy Technology Data Exchange (ETDEWEB)

    Camez, C.

    2004-09-15

    One of the applications justifying more the use of renewable energies is the electrification of isolated sites. Sites for which connection to the local distribution network is being out of price or impossible. These sites are most of the time electrified with a diesel generator. This technology is tested and mature however two major disadvantages can be raised here: necessary routine maintenance and the fuel provisioning management. Photovoltaic system can answer here the request and little solar home systems (100 Watts) are installed. In the particular situation of a village electrification, the consumers quickly see in these systems a strong limitation with the development. Reliability, evolution, comfort, service are the masters words here, the development of a micro industry also seems necessary to a village development. It is then necessary to propose a micro network type solution. Such a generator cannot be think completely photovoltaic, presenting an important over-cost compared to a conventional solution. The idea of conventional and renewable energy coupling is born. Such a multi-source system: hybrid, implies an increased sizing and management complexity. An analysis software tool was developed: HYPSIM, this one makes it possible to optimize the operation of such a system. HYPSIM made possible to - Justify the relevance of a photovoltaic/diesel generator/batteries system. - Conclude on the sizing criteria to be observed. (author)

  14. Development of a module combustor biomass-motor Stirling applied to a isolated generation system and based on induction generator; Desenvolvimento de um modulo combustor biomassa-motor Stirling aplicado a sistemas de geracao isolada e baseados em gerador de inducao

    Energy Technology Data Exchange (ETDEWEB)

    Wilke, Humberto; Lora, Electo Eduardo Silva [Universidade Federal de Itajuba (UNIFEI), MG (Brazil). Nucleo de Excelencia em Geracao Termeletrica e Distribuida

    2004-07-01

    In some areas in Brazil the great distance of the generating and consuming centers, together with the low consumption of electric energy of these areas, they make unfeasible investments with transmission and distribution. The use of the biomass as fuel in the distributed generation, is low pollutions, it is renewable, besides possessing a low cost when compared to the fossil fuels [Barros, 2003]. By the way the generation distributed through renewable fuels, becomes an attractive solution for generation in distant and isolated areas of the electric system. Inside of this context, this paper proposes the use of a module combustor, biomass-motor Stirling, based on induction generator, applied to isolated areas, such as the north and northeast areas of the Brazil. (author)

  15. Comparative Prevalence of Immune Evasion Complex Genes Associated with β-Hemolysin Converting Bacteriophages in MRSA ST5 Isolates from Swine, Swine Facilities, Humans with Swine Contact, and Humans with No Swine Contact.

    Directory of Open Access Journals (Sweden)

    Samantha J Hau

    Full Text Available Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA draws concern from the public health community because in some countries these organisms may represent the largest reservoir of MRSA outside hospital settings. Recent studies indicate LA-MRSA strains from swine are more genetically diverse than the first reported sequence type ST398. In the US, a diverse population of LA-MRSA is found including organisms of the ST398, ST9, and ST5 lineages. Occurrence of ST5 MRSA in swine is of particular concern since ST5 is among the most prevalent lineages causing clinical infections in humans. The prominence of ST5 in clinical disease is believed to result from acquisition of bacteriophages containing virulence or host-adapted genes including the immune-evasion cluster (IEC genes carried by β-hemolysin converting bacteriophages, whose absence in LA-MRSA ST398 is thought to contribute to reduced rates of human infection and transmission associated with this lineage. The goal of this study was to investigate the prevalence of IEC genes associated with β-hemolysin converting bacteriophages in MRSA ST5 isolates obtained from agricultural sources, including swine, swine facilities, and humans with short- or long-term swine exposure. To gain a broader perspective, the prevalence of these genes in LA-MRSA ST5 strains was compared to the prevalence in clinical MRSA ST5 strains from humans with no known exposure to swine. IEC genes were not present in any of the tested MRSA ST5 strains from agricultural sources and the β-hemolysin gene was intact in these strains, indicating the bacteriophage's absence. In contrast, the prevalence of the β-hemolysin converting bacteriophage in MRSA ST5 strains from humans with no exposure to swine was 90.4%. The absence of β-hemolysin converting bacteriophage in LA-MRSA ST5 isolates is consistent with previous reports evaluating ST398 strains and provides genetic evidence indicating LA-MRSA ST5 isolates

  16. Canastota Renewable Energy Facility Project

    Energy Technology Data Exchange (ETDEWEB)

    Blake, Jillian; Hunt, Allen

    2013-12-13

    The project was implemented at the Madison County Landfill located in the Town of Lincoln, Madison County, New York. Madison County has owned and operated the solid waste and recycling facilities at the Buyea Road site since 1974. At the onset of the project, the County owned and operated facilities there to include three separate landfills, a residential solid waste disposal and recycled material drop-off facility, a recycling facility and associated administrative, support and environmental control facilities. This putrescible waste undergoes anaerobic decomposition within the waste mass and generates landfill gas, which is approximately 50% methane. In order to recover this gas, the landfill was equipped with gas collection systems on both the east and west sides of Buyea Road which bring the gas to a central point for destruction. In order to derive a beneficial use from the collected landfill gases, the County decided to issue a Request for Proposals (RFP) for the future use of the generated gas.

  17. Isolation and characterization of a metastatic hybrid cell line generated by ER negative and ER positive breast cancer cells in mouse bone marrow.

    Directory of Open Access Journals (Sweden)

    Keya De Mukhopadhyay

    Full Text Available BACKGROUND: The origin and the contribution of breast tumor heterogeneity to its progression are not clear. We investigated the effect of a growing orthotopic tumor formed by an aggressive estrogen receptor (ER-negative breast cancer cell line on the metastatic potential of a less aggressive ER-positive breast cancer cell line for the elucidation of how the presence of heterogeneous cancer cells might affect each other's metastatic behavior. METHODS: ER positive ZR-75-1/GFP/puro cells, resistant to puromycin and non-tumorigenic/non-metastatic without exogenous estrogen supplementation, were injected intracardiacally into mice bearing growing orthotopic tumors, formed by ER negative MDA-MB-231/GFP/Neo cells resistant to G418. A variant cell line B6, containing both estrogen-dependent and -independent cells, were isolated from GFP expressing cells in the bone marrow and re-inoculated in nude mice to generate an estrogen-independent cell line B6TC. RESULTS: The presence of ER negative orthotopic tumors resulted in bone metastasis of ZR-75-1 without estrogen supplementation. The newly established B6TC cell line was tumorigenic without estrogen supplementation and resistant to both puromycin and G418 suggesting its origin from the fusion of MDA-MB-231/GFP/Neo and ZR-75-1/GFP/puro in the mouse bone marrow. Compared to parental cells, B6TC cells were more metastatic to lung and bone after intracardiac inoculation. More significantly, B6TC mice also developed brain metastasis, which was not observed in the MDA-MB-231/GFP/Neo cell-inoculated mice. Low expression of ERα and CD24, and high expression of EMT-related markers such as Vimentin, CXCR4, and Integrin-β1 along with high CD44 and ALDH expression indicated stem cell-like characteristics of B6TC. Gene microarray analysis demonstrated a significantly different gene expression profile of B6TC in comparison to those of parental cell lines. CONCLUSIONS: Spontaneous generation of the novel hybrid cell

  18. Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

    Directory of Open Access Journals (Sweden)

    Jakob Hedegaard

    Full Text Available Formalin-fixed, paraffin-embedded (FFPE tissues are an invaluable resource for clinical research. However, nucleic acids extracted from FFPE tissues are fragmented and chemically modified making them challenging to use in molecular studies. We analysed 23 fresh-frozen (FF, 35 FFPE and 38 paired FF/FFPE specimens, representing six different human tissue types (bladder, prostate and colon carcinoma; liver and colon normal tissue; reactive tonsil in order to examine the potential use of FFPE samples in next-generation sequencing (NGS based retrospective and prospective clinical studies. Two methods for DNA and three methods for RNA extraction from FFPE tissues were compared and were found to affect nucleic acid quantity and quality. DNA and RNA from selected FFPE and paired FF/FFPE specimens were used for exome and transcriptome analysis. Preparations of DNA Exome-Seq libraries was more challenging (29.5% success than that of RNA-Seq libraries, presumably because of modifications to FFPE tissue-derived DNA. Libraries could still be prepared from RNA isolated from two-decade old FFPE tissues. Data were analysed using the CLC Bio Genomics Workbench and revealed systematic differences between FF and FFPE tissue-derived nucleic acid libraries. In spite of this, pairwise analysis of DNA Exome-Seq data showed concordance for 70-80% of variants in FF and FFPE samples stored for fewer than three years. RNA-Seq data showed high correlation of expression profiles in FF/FFPE pairs (Pearson Correlations of 0.90 +/- 0.05, irrespective of storage time (up to 244 months and tissue type. A common set of 1,494 genes was identified with expression profiles that were significantly different between paired FF and FFPE samples irrespective of tissue type. Our results are promising and suggest that NGS can be used to study FFPE specimens in both prospective and retrospective archive-based studies in which FF specimens are not available.

  19. Experimental Component Characterization, Monte-Carlo-Based Image Generation and Source Reconstruction for the Neutron Imaging System of the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Barrera, C A; Moran, M J

    2007-08-21

    The Neutron Imaging System (NIS) is one of seven ignition target diagnostics under development for the National Ignition Facility. The NIS is required to record hot-spot (13-15 MeV) and downscattered (6-10 MeV) images with a resolution of 10 microns and a signal-to-noise ratio (SNR) of 10 at the 20% contour. The NIS is a valuable diagnostic since the downscattered neutrons reveal the spatial distribution of the cold fuel during an ignition attempt, providing important information in the case of a failed implosion. The present study explores the parameter space of several line-of-sight (LOS) configurations that could serve as the basis for the final design. Six commercially available organic scintillators were experimentally characterized for their light emission decay profile and neutron sensitivity. The samples showed a long lived decay component that makes direct recording of a downscattered image impossible. The two best candidates for the NIS detector material are: EJ232 (BC422) plastic fibers or capillaries filled with EJ399B. A Monte Carlo-based end-to-end model of the NIS was developed to study the imaging capabilities of several LOS configurations and verify that the recovered sources meet the design requirements. The model includes accurate neutron source distributions, aperture geometries (square pinhole, triangular wedge, mini-penumbral, annular and penumbral), their point spread functions, and a pixelated scintillator detector. The modeling results show that a useful downscattered image can be obtained by recording the primary peak and the downscattered images, and then subtracting a decayed version of the former from the latter. The difference images need to be deconvolved in order to obtain accurate source distributions. The images are processed using a frequency-space modified-regularization algorithm and low-pass filtering. The resolution and SNR of these sources are quantified by using two surrogate sources. The simulations show that all LOS

  20. Facile Route to Generate Fuel Oil via Catalytic Pyrolysis of Waste Polypropylene Bags: Towards Waste Management of >20 μm Plastic Bags

    Directory of Open Access Journals (Sweden)

    Neeraj Mishra

    2014-01-01

    Full Text Available A novel strategy of waste recycling of polypropylene plastics (PP bags for generation of commercially viable byproducts using nanoforms of nickel as catalyst is presented in this work. After pyrolysis of waste PP bags (>20 μm under continuous argon flow, 90% conversion efficiency to high petroleum oil was observed at 550°C. To assess the physicochemical attributes of formed oil, flash point, pour point, viscosity, specific gravity, heating value, and density were also measured and found to be very close to ideal values of commercial fuel oil. Moreover, GC-MS was used to resolve the range of trace mass hydrocarbon present in the liquefied hydrocarbon. Our robust recycling system can be exploited as economical technique to solve the nuisance of waste plastic hazardous to ecosystem.

  1. Asian Facilities

    Science.gov (United States)

    Nakahata, M.

    2011-04-01

    Asian underground facilities are reviewed. The YangYang underground Laboratory in Korea and the Kamioka observatory in Japan are operational and several astrophysical experiments are running. Indian Neutrino Observatory(INO) and China JinPing Underground Laboratory (CJPL) are under construction and underground experiments are being prepared. Current activities and future prospects at those underground sites are described.

  2. Poster: The EURISOL Beta-beam facility

    CERN Document Server

    The beta-beam concept for the generation of an electron (anti-)neutrino beam was proposed by Piero Zucchelli (CERN) in 2002. A first study of the possibility of using the existing CERN machines for the acceleration for radioactive ions to a relativistic gamma of roughly 100, for later storage in a new decay ring of approximately the size of SPS, was made in 2002. The results from this very first short study were very encouraging.In 2004 it was decided to incorporate a design study for the beta-beam within the EURISOL DS proposal. EURISOL is a project name for a next-generation radioactive beam facility based on the ISOL method for the production of intense radioactive beams for nuclear physics, astrophysics and other applications. The proposal was accepted with the beta-beam task as an integral part. The design study officially started 1 February 2005 and will run for 4 years resulting in a conceptual design report for a beta-beam facility.

  3. CLIC Test Facility 3

    CERN Multimedia

    Kossyvakis, I; Faus-golfe, A; Nguyen, F

    2007-01-01

    The design of CLIC is based on a two-beam scheme, where short pulses of high power 30 GHz RF are extracted from a drive beam running parallel to the main beam. The 3rd generation CLIC Test Facility (CTF3) will demonstrate the generation of the drive beam with the appropriate time structure, the extraction of 30 GHz RF power from this beam, as well as acceleration of a probe beam with 30 GHz RF cavities. The project makes maximum use of existing equipment and infrastructure of the LPI complex, which became available after the closure of LEP.

  4. Impact of revised CLSI breakpoints for susceptibility to third-generation cephalosporins and carbapenems among Enterobacteriaceae isolates in the Asia-Pacific region: results from the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2002-2010.

    Science.gov (United States)

    Huang, Chi-Chang; Chen, Yao-Shen; Toh, Han-Siong; Lee, Yu-Lin; Liu, Yuag-Meng; Ho, Cheng-Mao; Lu, Po-Liang; Liu, Chun-Eng; Chen, Yen-Hsu; Wang, Jen-Hsien; Tang, Hung-Jen; Yu, Kwok-Woon; Liu, Yung-Ching; Chuang, Yin-Ching; Xu, Yingchun; Ni, Yuxing; Ko, Wen-Chien; Hsueh, Po-Ren

    2012-06-01

    This study examined the rates of susceptibility to third-generation cephalosporins and carbapenems among Enterobacteriaceae isolates that had been obtained from patients with intraabdominal infections in the Asia-Pacific region as part of the Study for Monitoring Antimicrobial Resistance Trends (SMART). Susceptibility profiles obtained using 2009 Clinical and Laboratory Standards Institute (CLSI) breakpoints were compared with those obtained using the 2011 CLSI breakpoints. From 2002 to 2010, Escherichia coli and Klebsiella pneumoniae together accounted for more than 60% of the 13714 Enterobacteriaceae isolates analyzed during the study period. Extended-spectrum β-lactamase (ESBL) producers comprised 28.2% of E. coli isolates and 22.1% of K. pneumoniae isolates in the Asia-Pacific region, with China (55.6% and 33.7%, respectively) and Thailand (43.1% and 40.7%, respectively) having the highest proportions of ESBL producers. Based on the 2011 CLSI criteria, 77.2% of the Enterobacteriaceae isolates, 40.4% of ESBL-producing E. coli, and 25.2% of ESBL-producing K. pneumoniae isolates were susceptible to ceftazidime. Carbapenems showed in vitro activity against >90% of Enterobacteriaceae isolates in all participating countries, except for ertapenem in South Korea (susceptibility rate 82.2%). Marked differences (>5%) in susceptibility of ESBL-producing E. coli and K. pneumoniae isolates to carbapenems were noted between the profiles obtained using the 2009 CLSI criteria and those using the 2011 CLSI criteria. Continuous monitoring of antimicrobial resistance is necessary in the Asia-Pacific region.

  5. Iodidimonas muriae gen. nov., sp. nov., an aerobic iodide-oxidizing bacterium isolated from brine of a natural gas and iodine recovery facility, and proposals of Iodidimonadaceae fam. nov., Iodidimonadales ord. nov., Emcibacteraceae fam. nov. and Emcibacterales ord. nov.

    Science.gov (United States)

    Iino, Takao; Ohkuma, Moriya; Kamagata, Yoichi; Amachi, Seigo

    2016-12-01

    A chemo-organotrophic iodide (I-)-oxidizing bacterial strain, C-3T, isolated from natural gas brine of an iodine recovery facility in Kujukuri, Chiba, Japan, was characterized for representation of a novel species in the class Alphaproteobacteria. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbours of strain C-3T were members of the genera Eilatimonas, Kordiimonas, Rhodothalassium and Temperatibacter with 88-91 % sequence similarity. Cells of strain C-3T were aerobic, Gram-staining-negative, non-sporulating and rod-shaped (1.3-3.6 µm in length). Strain C-3T grew optimally at 30 °C, pH 7.5 and with 3 % NaCl (w/v). Iodide oxidation to form molecular iodine (I2) was a unique trait for strain C-3T, whereas the strain did not utilize iodide as a sole electron donor for chemolithoautotrophic growth. The major isoprenoid quinone was Q-10. The major cellular fatty acids were C18 : 1ω7c and C16 : 1ω5c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The G+C content of the genomic DNA was 58.5 mol%. Iodide oxidation and the major cellular fatty acids composition distinguished strain C-3T from phylogenetically related bacteria. On the basis of the phenotypic features and the phylogenetic position, a novel genus and species are proposed for strain C-3T (=JCM 17843T=LMG 28660T), to be named Iodidimonas muriae gen. nov., sp. nov. We also propose to place the distinct sublineages of the genera Iodidimonasgen. nov. and Emcibacter in the orders Iodidimonadales ord. nov. and Emcibacterales ord. nov., respectively, because these genera are located far apart from the order Kordiimonadales and form the distinct lineage in the class Alphaproteobacteria.

  6. Information related to low-level mixed waste inventory, characteristics, generation, and facility assessment for treatment, storage, and disposal alternatives considered in the U.S. Department of Energy Waste Management Programmatic Environmental Impact Statement

    Energy Technology Data Exchange (ETDEWEB)

    Wilkins, B.D.; Dolak, D.A.; Wang, Y.Y.; Meshkov, N.K.

    1996-12-01

    This report was prepared to support the analysis of risks and costs associated with the proposed treatment of low-level mixed waste (LLMW) under management of the US Department of Energy (DOE). The various waste management alternatives for treatment of LLMW have been defined in the DOE`s Office of Waste Management Programmatic Environmental Impact Statement. This technical memorandum estimates the waste material throughput expected at each proposed LLMW treatment facility and analyzes potential radiological and chemical releases at each DOE site resulting from treatment of these wastes. Models have been developed to generate site-dependent radiological profiles and waste-stream-dependent chemical profiles for these wastes. Current site-dependent inventories and estimates for future generation of LLMW have been obtained from DOE`s 1994 Mixed Waste Inventory Report (MWIR-2). Using treatment procedures developed by the Mixed Waste Treatment Project, the MWIR-2 database was analyzed to provide waste throughput and emission estimates for each of the different waste types assessed in this report. Uncertainties in the estimates at each site are discussed for waste material throughputs and radiological and chemical releases.

  7. Pressurized burner test facility

    Energy Technology Data Exchange (ETDEWEB)

    Maloney, D.J.; Norton, T.S.; Hadley, M.A.

    1993-09-01

    The US Department of Energy`s METC has recently completed construction and commissioning of a new high-pressure combustion research facility. Utilities servicing the facility enable combustion tests at scales up to 3 MW (10 MM Btu/h) and pressures in excess of 3000 kPa (30 atm). These include a preheated, high-pressure air supply that can deliver up to 1.7 kg/s (3.7 lbs/s) of combustion air, and a high-pressure, natural gas compressor that can deliver 0.8 kg/s (.19 lbs/s). In the summer of 1994 METC`s syngas generator is scheduled to come on line, at which time combustion tests on a range of fuel gases from low to medium to high heating values will be possible. The syngas generator will simulate a range of fuel gas compositions characteristic of coal gasification product streams. As part of the combustion facility, a high-pressure burner test facility is currently being constructed to support the development of gas turbine combustion systems fired on natural gas and coal-derived gaseous fuels containing fuel-bound nitrogen. The facility, illustrated in Figure 1, is a 61-centimeter (24-inch) diameter, refractory-lined vessel of modular construction, offering the flexibility to test a variety of NO{sub x} control concepts. Burner test modules are sandwiched between gas inlet and sampling plenums with a maximum combustion test zone of 2.2 m (90 inches) in length. Modules are custom designed for specific burners.

  8. Emission Facilities - Erosion & Sediment Control Facilities

    Data.gov (United States)

    NSGIC Education | GIS Inventory — An Erosion and Sediment Control Facility is a DEP primary facility type related to the Water Pollution Control program. The following sub-facility types related to...

  9. Status of the SXFEL Facility

    Directory of Open Access Journals (Sweden)

    Zhentang Zhao

    2017-06-01

    Full Text Available The Shanghai soft X-ray Free-Electron Laser facility (SXFEL is being developed in two steps; the SXFEL test facility (SXFEL-TF, and the SXFEL user facility (SXFEL-UF. The SXFEL-TF is a critical development step towards the construction a soft X-ray FEL user facility in China, and is under commissioning at the Shanghai Synchrotron Radiation Facility (SSRF campus. The test facility is going to generate 8.8 nm FEL radiation using an 840 MeV electron linac passing through the two-stage cascaded HGHG-HGHG or EEHG-HGHG (high-gain harmonic generation, echo-enabled harmonic generation scheme. The construction of the SXFEL-TF started at the end of 2014. Its accelerator tunnel and klystron gallery were ready for equipment installation in April 2016, and the installation of the SXFEL-TF linac and radiator undulators were completed by the end of 2016. In the meantime, the SXFEL-UF, with a designated wavelength in the water window region, began construction in November 2016. This was based on upgrading the linac energy to 1.5 GeV, and the building of a second undulator line and five experimental end-stations. Construction status and the future plans of the SXFEL are reported in this paper.

  10. FY 1998 evaluation/analysis by site of the data collected in the field test project for photovoltaic power generation for public facilities. 2/4; 1998 nendo kokyo shisetsu nadoyo taiyoko hatsuden field test jigyo ni okeru shushu data hyoka kaiseki kaku site betsu 2/4

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-07-01

    The data collected monthly in the FY 1998 photovoltaic power generation field test project for public facilities were evaluated/analyzed by site. The data items are the flux of global solar radiation/global radiation on an inclined surface/effective global radiation on an inclined surface (each: kWh/m{sup 2}), mean temperature, and array electric energy/system consumption power/load electric energy/system supply load/back flow electric energy (each: kWh). Others are times of linkage protection movement, linkage disconnection time (min), system operation time (min), independent supply load (kWh), equivalent array/equivalent system operation time, array/system output coefficient, system utilization rate/power generation efficiency, INV effective efficiency, and INV load factor. The sites for survey are school (junior and senior high school/elementary school/kindergarten/nursery), lifelong education facilities (technical training center/forestry study center), government office (government office building), environmental public facilities (water purification plant/disaster-prevention center/park), welfare facilities (special nursing care center for the elderly), public facilities (community center/martial arts center), cultural facilities, factory in area, etc. (NEDO)

  11. Thomas Jefferson National Accelerator Facility

    Energy Technology Data Exchange (ETDEWEB)

    Grames, Joseph; Higinbotham, Douglas; Montgomery, Hugh

    2010-09-08

    The Thomas Jefferson National Accelerator Facility (Jefferson Lab) in Newport News, Virginia, USA, is one of ten national laboratories under the aegis of the Office of Science of the U.S. Department of Energy (DOE). It is managed and operated by Jefferson Science Associates, LLC. The primary facility at Jefferson Lab is the Continuous Electron Beam Accelerator Facility (CEBAF) as shown in an aerial photograph in Figure 1. Jefferson Lab was created in 1984 as CEBAF and started operations for physics in 1995. The accelerator uses superconducting radio-frequency (srf) techniques to generate high-quality beams of electrons with high-intensity, well-controlled polarization. The technology has enabled ancillary facilities to be created. The CEBAF facility is used by an international user community of more than 1200 physicists for a program of exploration and study of nuclear, hadronic matter, the strong interaction and quantum chromodynamics. Additionally, the exceptional quality of the beams facilitates studies of the fundamental symmetries of nature, which complement those of atomic physics on the one hand and of high-energy particle physics on the other. The facility is in the midst of a project to double the energy of the facility and to enhance and expand its experimental facilities. Studies are also pursued with a Free-Electron Laser produced by an energy-recovering linear accelerator.

  12. Air Quality Facilities

    Data.gov (United States)

    Iowa State University GIS Support and Research FacilityFacilities with operating permits for Title V of the Federal Clean Air Act, as well as facilities required to submit an air emissions inventory, and other facilities...

  13. Theme: Laboratory Facilities Improvement.

    Science.gov (United States)

    Miller, Glen M.; And Others

    1993-01-01

    Includes "Laboratory Facilities Improvement" (Miller); "Remodeling Laboratories for Agriscience Instruction" (Newman, Johnson); "Planning for Change" (Mulcahy); "Laboratory Facilities Improvement for Technology Transfer" (Harper); "Facilities for Agriscience Instruction" (Agnew et al.); "Laboratory Facility Improvement" (Boren, Dwyer); and…

  14. Electricity generation using vegetal oils: the implantation model for the isolated communities in the Amazon Region; Geracao de eletricidade a partir de oleos vegetais: um modelo de implantacao para comunidades isoladas amazonicas

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira; Silva, Orlando Cristiano; Velazquez, Silvia M.S.G.; Monteiro, Maria Beatriz C.A.; Silotto, Carlos Eduardo G. [Centro Nacional de Referencia em Biomassa - CENBIO, Sao Paulo, SP (Brazil)

    2004-07-01

    The territorial occupation from the Amazon region is characterized by isolated communities, which makes the conventional electric distribution economically and technically impracticable. To solve the electric problem of these communities, it is necessary for them to produce their own fuel in order to generate electric energy and promote the development. These are the premises that have guided the elaboration of the project PROVEGAM - which has as objective, the installation and performance testing of a conventional diesel engine, adapted to operate with palm oil 'in natura' at the community of Vila Soledade, city of Moju, Para State. Vila Soledade is an isolated community that has, approximately, 700 inhabitants. The PROVEGAM project installed a diesel generator adapted with a conversion kit to operate with 'in natura' palm oil. The operation begins and finishes with diesel oil, in order to heat the palm oil and cleaning possible residues. During the diesel generator installation, diesel oil emissions and performance were compared with the palm oil. Analysing the results, this electric model of generating energy is already recommended to be implemented in others Amazon region communities. (author)

  15. Seismic Analysis of a Viscoelastic Damping Isolator

    Directory of Open Access Journals (Sweden)

    Bo-Wun Huang

    2015-01-01

    Full Text Available Seismic prevention issues are discussed much more seriously around the world after Fukushima earthquake, Japan, April 2011, especially for those countries which are near the earthquake zone. Approximately 1.8×1012 kilograms of explosive energy will be released from a magnitude 9 earthquake. It destroys most of the unprotected infrastructure within several tens of miles in diameter from the epicenter. People can feel the earthquake even if living hundreds of miles away. This study is a seismic simulation analysis for an innovated and improved design of viscoelastic damping isolator, which can be more effectively applied to earthquake prevention and damage reduction of high-rise buildings, roads, bridges, power generation facilities, and so forth, from earthquake disaster. Solidworks graphic software is used to draw the 3D geometric model of the viscoelastic isolator. The dynamic behavior of the viscoelastic isolator through shock impact of specific earthquake loading, recorded by a seismometer, is obtained via ANSYS finite element package. The amplitude of the isolator is quickly reduced by the viscoelastic material in the device and is shown in a time response diagram. The result of this analysis can be a crucial reference when improving the design of a seismic isolator.

  16. Rapid generation of a well-matched vaccine seed from a modern influenza A virus primary isolate without recourse to eggs

    NARCIS (Netherlands)

    Hartgroves, L.C.S.; Koudstaal, W.; McLeod, C.; Moncorgé, O.; Thompson, C.I.; Ellis, J.; Bull, C.; Havenga, M.J.E.; Goudsmit, J.; Barclay, W.S.

    2010-01-01

    Most influenza vaccines are produced in chicken eggs but recent human influenza strains often do not grow well in this substrate. The PER.C6® cell line is an alternative platform for vaccine production. Here we demonstrate that PER.C6 cells faithfully propagate recent clinical isolates, without sele

  17. Normanskill Hydroelectric Facility Feasibility Assessment

    Energy Technology Data Exchange (ETDEWEB)

    Besha, J.A.

    1979-01-01

    A study was conducted to determine the feasibility of installing a hydroelectric generating facility at an existing dam of the Normanskill Reservoir in NY. Evaluation of the hydrologic, technical, economic, legal, instrumental and environmental factors led to the conclusion that the project is feasible and advantageous. The proposed project has a present worth net cost of $3,099,800. The benefit cost ratio is 2.36. It is estimated that the proposed hydroelectric generating facility at the French's Mills site, City of Watervliet Reservoir will replace approximately 6,000 barrels of foreign oil per year. (LCL)

  18. NB Power`s fish handling facilities

    Energy Technology Data Exchange (ETDEWEB)

    Hickman, C. N. [New Brunswick Power, Fredericton, NB (Canada)

    1995-12-31

    Fish handling facilities at the various hydroelectric power generating stations of New Brunswick Power on the St. John River, were described. The facilities described included the fish hoist at the Beechwood Generating Station , the fish ladder at the Tobique Generating Station and a trap and truck operation at the Mactaquac Station. Details of operating arrangements were provided. Operating issues, solutions and ongoing efforts to improve operations further, were discussed. 4 refs., 4 figs.

  19. Educational Facilities.

    Science.gov (United States)

    Mostaedi, Arian

    The aim of this book is to offer a balanced overview of architects considered to be worthy representatives of this generation's building forms, and who have placed their creativity at the service of new educational establishments. It offers a selection of 19 designs in this field of architecture, illustrated with numerous photographs, plans, and…

  20. Facility effluent monitoring plan for the 325 Facility

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    The Applied Chemistry Laboratory (325 Facility) houses radiochemistry research, radioanalytical service, radiochemical process development, and hazardous and mixed hazardous waste treatment activities. The laboratories and specialized facilities enable work ranging from that with nonradioactive materials to work with picogram to kilogram quantities of fissionable materials and up to megacurie quantities of other radionuclides. The special facilities include two shielded hot-cell areas that provide for process development or analytical chemistry work with highly radioactive materials, and a waste treatment facility for processing hazardous, mixed, low-level, and transuranic wastes generated by Pacific Northwest Laboratory. Radioactive material storage and usage occur throughout the facility and include a large number of isotopes. This material is in several forms, including solid, liquid, particulate, and gas. Some of these materials are also heated during testing which can produce vapors. The research activities have been assigned to the following activity designations: High-Level Hot Cell, Hazardous Waste Treatment Unit, Waste Form Development, Special Testing Projects, Chemical Process Development, Analytical Hot Cell, and Analytical Chemistry. The following summarizes the airborne and liquid effluents and the results of the Facility Effluent Monitoring Plan (FEMP) determination for the facility. The complete monitoring plan includes characterization of effluent streams, monitoring/sampling design criteria, a description of the monitoring systems and sample analysis, and quality assurance requirements.

  1. The Zwicky Transient Facility

    CERN Document Server

    Bellm, Eric C

    2014-01-01

    The Zwicky Transient Facility (ZTF) is a next-generation optical synoptic survey that builds on the experience and infrastructure of the Palomar Transient Factory (PTF). Using a new 47 deg$^2$ survey camera, ZTF will survey more than an order of magnitude faster than PTF to discover rare transients and variables. I describe the survey and the camera design. Searches for young supernovae, fast transients, counterparts to gravitational-wave detections, and rare variables will benefit from ZTF's high cadence, wide area survey.

  2. Subsurface Facility System Description Document

    Energy Technology Data Exchange (ETDEWEB)

    Eric Loros

    2001-07-31

    The Subsurface Facility System encompasses the location, arrangement, size, and spacing of the underground openings. This subsurface system includes accesses, alcoves, and drifts. This system provides access to the underground, provides for the emplacement of waste packages, provides openings to allow safe and secure work conditions, and interfaces with the natural barrier. This system includes what is now the Exploratory Studies Facility. The Subsurface Facility System physical location and general arrangement help support the long-term waste isolation objectives of the repository. The Subsurface Facility System locates the repository openings away from main traces of major faults, away from exposure to erosion, above the probable maximum flood elevation, and above the water table. The general arrangement, size, and spacing of the emplacement drifts support disposal of the entire inventory of waste packages based on the emplacement strategy. The Subsurface Facility System provides access ramps to safely facilitate development and emplacement operations. The Subsurface Facility System supports the development and emplacement operations by providing subsurface space for such systems as ventilation, utilities, safety, monitoring, and transportation.

  3. Waste Isolation Pilot Plant Site Environmental Report for calendar year 1989

    Energy Technology Data Exchange (ETDEWEB)

    1989-01-01

    This is the 1989 Site Environmental Report (SER) for the Waste Isolation Pilot Plant (WIPP) in southeastern New Mexico. The WIPP is a government owned and contractor-operated facility. The WIPP project is operated by Westinghouse Electric Corporation for the US Department of Energy (DOE). The mission of the WIPP is to provide a research and development facility to demonstrate the safe disposal of transuranic (TRU) waste generated by the defense activities of the US Government. This report provides a comprehensive description of environmental activities at the WIPP during calendar year 1989. The WIPP facility will not receive waste until all concerns affecting opening the WIPP are addressed to the satisfaction of the Secretary of Energy. Therefore, this report describes the status of the preoperational activities of the Radiological Environmental Surveillance (RES) program, which are outlined in the Radiological Baseline Program for the Waste Isolation Pilot Plant (WTSD-TME-057). 72 refs., 13 figs., 20 tabs.

  4. The National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Miller, G H; Moses, E I; Wuest, C R

    2004-06-03

    The National Ignition Facility (NIF) at Lawrence Livermore National Laboratory is a stadium-sized facility that, when completed in 2008, will contain a 192-beam, 1.8- Megajoule, 500-Terawatt, ultraviolet laser system together with a 10-meter-diameter target chamber and room for 100 diagnostics. NIF is the world's largest and most energetic laser experimental system and will provide a scientific center to study inertial confinement fusion and matter at extreme energy densities and pressures. NIF's energetic laser beams will compress fusion targets to conditions required for thermonuclear burn, liberating more energy than required to initiate the fusion reactions. Other NIF experiments will study physical processes at temperatures approaching 10{sup 8} K and 10{sup 11} bar; conditions that exist naturally only in the interior of stars and planets. NIF has completed the first phases of its laser commissioning program. The first four beams of NIF have generated 106 kilojoules in 23-ns pulses of infrared light and over 16 kJ in 3.5- ns pulses at the third harmonic (351 nm). NIF's target experimental systems are being commissioned and experiments have begun. This paper provides a detailed look the NIF laser systems, laser and optical performance, and results from recent laser commissioning shots. We follow this with a discussion of NIF's high-energy-density and inertial fusion experimental capabilities, the first experiments on NIF, and plans for future capabilities of this unique facility.

  5. Uncovering a Microbial Enigma: Isolation and Characterization of the Streamer-Generating, Iron-Oxidizing, Acidophilic Bacterium “Ferrovum myxofaciens”

    Science.gov (United States)

    Hallberg, Kevin B.; Hedrich, Sabrina

    2014-01-01

    A betaproteobacterium, shown by molecular techniques to have widespread global distribution in extremely acidic (pH 2 to 4) ferruginous mine waters and also to be a major component of “acid streamer” growths in mine-impacted water bodies, has proven to be recalcitrant to enrichment and isolation. A modified “overlay” solid medium was devised and used to isolate this bacterium from a number of mine water samples. The physiological and phylogenetic characteristics of a pure culture of an isolate from an abandoned copper mine (“Ferrovum myxofaciens” strain P3G) have been elucidated. “F. myxofaciens” is an extremely acidophilic, psychrotolerant obligate autotroph that appears to use only ferrous iron as an electron donor and oxygen as an electron acceptor. It appears to use the Calvin-Benson-Bassham pathway to fix CO2 and is diazotrophic. It also produces copious amounts of extracellular polymeric materials that cause cells to attach to each other (and to form small streamer-like growth in vitro) and to different solid surfaces. “F. myxofaciens” can catalyze the oxidative dissolution of pyrite and, like many other acidophiles, is tolerant of many (cationic) transition metals. “F. myxofaciens” and related clone sequences form a monophyletic group within the Betaproteobacteria distantly related to classified orders, with genera of the family Nitrosomonadaceae (lithoautotrophic, ammonium-oxidizing neutrophiles) as the closest relatives. On the basis of the phylogenetic and phenotypic differences of “F. myxofaciens” and other Betaproteobacteria, a new family, “Ferrovaceae,” and order, “Ferrovales,” within the class Betaproteobacteria are proposed. “F. myxofaciens” is the first extreme acidophile to be described in the class Betaproteobacteria. PMID:24242243

  6. The New LOTIS Test Facility

    Science.gov (United States)

    Bell, R. M.; Cuzner, G.; Eugeni, C.; Hutchison, S. B.; Merrick, A. J.; Robins, G. C.; Bailey, S. H.; Ceurden, B.; Hagen, J.; Kenagy, K.; Martin, H. M.; Tuell, M.; Ward, M.; West, S. C.

    2008-01-01

    The Large Optical Test and Integration Site (LOTIS) at the Lockheed Martin Space Systems Company in Sunnyvale, CA is designed for the verification and testing of optical systems. The facility consists of an 88 foot temperature stabilized vacuum chamber that also functions as a class 10k vertical flow cleanroom. Many problems were encountered in the design and construction phases. The industry capability to build large chambers is very weak. Through many delays and extra engineering efforts, the final product is very good. With 11 Thermal Conditioning Units and precision RTD s, temperature is uniform and stable within 1oF, providing an ideal environment for precision optical testing. Within this chamber and atop an advanced micro-g vibration-isolation bench is the 6.5 meter diameter LOTIS Collimator and Scene Generator, LOTIS alignment and support equipment. The optical payloads are also placed on the vibration bench in the chamber for testing. This optical system is designed to operate in both air and vacuum, providing test imagery in an adaptable suite of visible/near infrared (VNIR) and midwave infrared (MWIR) point sources, and combined bandwidth visible-through-MWIR point sources, for testing of large aperture optical payloads. The heart of the system is the LOTIS Collimator, a 6.5m f/15 telescope, which projects scenes with wavefront errors <85 nm rms out to a 0.75 mrad field of view (FOV). Using field lenses, performance can be extended to a maximum field of view of 3.2 mrad. The LOTIS Collimator incorporates an extensive integrated wavefront sensing and control system to verify the performance of the system.

  7. Identification of the first multi-exonic WDR72 deletion in isolated amelogenesis imperfecta, and generation of a WDR72-specific copy number screening tool.

    Science.gov (United States)

    Hentschel, Julia; Tatun, Dana; Parkhomchuk, Dmitri; Kurth, Ingo; Schimmel, Bettina; Heinrich-Weltzien, Roswitha; Bertzbach, Sabine; Peters, Hartmut; Beetz, Christian

    2016-09-15

    Amelogenesis imperfecta (AI) is a clinically and genetically heterogeneous disorder of tooth development which is due to aberrant deposition or composition of enamel. Both syndromic and isolated forms exist; they may be inherited in an X-linked, autosomal recessive, or autosomal dominant manner. WDR72 is one of ten currently known genes for recessive isolated AI; nine WDR72 mutations affecting single nucleotides have been described to date. Based on whole exome sequencing in a large consanguineous AI pedigree, we obtained evidence for presence of a multi-exonic WDR72 deletion. A home-made multiplex ligation-dependent probe amplification assay was used to confirm the aberration, to narrow its extent, and to identify heterozygous carriers. Our study extends the mutational spectrum for WDR72 to include large deletions, and supports a relevance of the previously proposed loss-of-function mechanism. It also introduces an easy-to-use and highly sensitive tool for detecting WDR72 copy number alterations.

  8. Prevalence of lactose fermenting coliforms resistant to third generation cephalosporins in cattle feedlot throughout a production cycle and molecular characterization of resistant isolates

    Science.gov (United States)

    Introduction: Increases in incidence of human infections caused by Enterobacteriaceae resistant to 3rd generation cephalosporins (3GC) have become a public health concern. The 3GC ceftiofur is commonly used for the therapeutic treatment of feedlot cattle but the impact this practice has on public h...

  9. New radiation protection calibration facility at CERN.

    Science.gov (United States)

    Brugger, Markus; Carbonez, Pierre; Pozzi, Fabio; Silari, Marco; Vincke, Helmut

    2014-10-01

    The CERN radiation protection group has designed a new state-of-the-art calibration laboratory to replace the present facility, which is >20 y old. The new laboratory, presently under construction, will be equipped with neutron and gamma sources, as well as an X-ray generator and a beta irradiator. The present work describes the project to design the facility, including the facility placement criteria, the 'point-zero' measurements and the shielding study performed via FLUKA Monte Carlo simulations.

  10. North Slope, Alaska ESI: FACILITY (Facility Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains data for oil field facilities for the North Slope of Alaska. Vector points in this data set represent oil field facility locations. This data...

  11. Prizes awarded in fiscal 1999 by the Minister for International Trade and Industry on factories having applied excellent energy management. Energy conservation by installing fuel cell power generation facilities utilizing methane gas generated from waste water treatment plants / Improvements toward a clean room and energy saving air conditioning system; 1999 nendo energy kanri yuryo kojo tsusho sangyo daijin hyosho jusho. 1999 nendo shigen energy sho chokan hyosho jusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-04-01

    In order to achieve energy conservation in a waste water treatment plant in a brewery factories, an anaerobic treatment facility was introduced, and a fuel cell power generation facility effectively utilizing methane gas generated from the anaerobic waste water treatment plant was installed. This has resulted in large reduction in the operating number of blowers in the waste water treatment facility of activated sludge system. In addition, electric power, steam, and hot water generated from the fuel cells are effectively utilized as the factory utility. In energy conservation in an optical communication device manufacturing factory, the fan filter unit system was adopted, having been changed from the circulation air conditioner, a conventional type air conditioning system. The present system is a circulation system integrating the fan with the filter, making it possible to circulate air in the room to perform control of temperature, humidity, and dust in a clean room. Thus, the system has eliminated the circulating air conditioner, and reduced the air conditioner capacity by 42% and power consumption by 58.6% compared with those in the conventional circulation system. (NEDO)

  12. CONSIDERATIONS FOR GROUT FORMULATIONS FOR FACILITY CLOSURES USING IN SITU STRATEGIES

    Energy Technology Data Exchange (ETDEWEB)

    Gladden, J.; Serrato, M.; Langton, C.; Long, T.; Blankenship, J.; Hannah, G.; Stubblefield, R.; Szilagyi, A.

    2010-08-25

    The U.S. Department of Energy (DOE) is conducting in situ closures (entombment) at a large number of facilities throughout the complex. Among the largest closure actions currently underway are the closures of the P and R Reactors at the Savannah River Site (SRS), near Aiken, South Carolina. In these facilities, subgrade open spaces are being stabilized with grout; this ensures the long term structural integrity of the facilities and permanently immobilizes and isolates residual contamination. The large size and structural complexity of these facilities present a wide variety of challenges for the identification and selection of appropriate fill materials. Considerations for grout formulations must account for flowability, long term stability, set times, heat generation and interactions with materials within the structure. The large size and configuration of the facility necessitates that grout must be pumped from the exterior to the spaces to be filled, which requires that the material must retain a high degree of flowability to move through piping without clogging while achieving the required leveling properties at the pour site. Set times and curing properties must be controlled to meet operations schedules, while not generating sufficient heat to compromise the properties of the fill material. The properties of residual materials can result in additional requirements for grout formulations. If significant quantities of aluminum are present in the facility, common formulations of highly alkaline grouts may not be appropriate because of the potential for hydrogen generation with the resultant risks. SRS is developing specialized inorganic grout formulations that are designed to address this issue. One circum-neutral chemical grout formulation identified for initial consideration did not possess the proper chemical characteristics, having exceptionally short set times and high heat of hydration. Research efforts are directed toward developing grout formulations

  13. An elusive hydridoaluminum(I) complex for facile C-H and C-O bond activation of ethers and access to its isolable hydridogallium(I) analogue: syntheses, structures, and theoretical studies.

    Science.gov (United States)

    Tan, Gengwen; Szilvási, Tibor; Inoue, Shigeyoshi; Blom, Burgert; Driess, Matthias

    2014-07-01

    The reaction of AlBr3 with 1 molar equiv of the chelating bis(N-heterocyclic carbene) ligand bis(N-Dipp-imidazole-2-ylidene)methylene (bisNHC, 1) affords [(bisNHC)AlBr2](+)Br(-) (2) as an ion pair in high yield, representing the first example of a bisNHC-Al(III) complex. Debromination of the latter with 1 molar equiv of K2Fe(CO)4 in tetrahydrofuran (THF) furnishes smoothly, in a redox reaction, the (bisNHC)(Br)Al[Fe(CO)4] complex 3, in which the Al(I) center is stabilized by the Fe(CO)4 moiety through Al(I):→Fe(0) coordination. Strikingly, the Br/H ligand exchange reactions of 3 using potassium hydride as a hydride source in THF or tetrahydropyran (THP) do not yield the anticipated hydridoaluminum(I) complex (bisNHC)Al(H)[Fe(CO)4] (4a) but instead lead to (bisNHC)Al(2-cyclo-OC4H7)[Fe(CO)4] (4) and (bisNHC)Al(2-cyclo-OC5H9)[Fe(CO)4] (5), respectively. The latter are generated via C-H bond activation at the α-carbon positions of THF and THP, respectively, in good yields with concomitant elimination of dihydrogen. This is the first example whereby a low-valent main-group hydrido complex facilitates metalation of sp(3) C-H bonds. Interestingly, when K[BHR3] (R = Et, sBu) is employed as a hydride source to react with 3 in THF, the reaction affords (bisNHC)Al(OnBu)[Fe(CO)4] (6) as the sole product through C-O bond activation and ring opening of THF. The mechanisms for these novel C-H and C-O bond activations mediated by the elusive hydridoaluminum(I) complex 4a were elucidated by density functional theory (DFT) calculations. In contrast, the analogous hydridogallium(I) complex (bisNHC)Ga(H)[Fe(CO)4] (9) can be obtained directly in high yield by the reaction of the (bisNHC)Ga(Cl)[Fe(CO)4] precursor 8 with 1 molar equiv of K[BHR3] (R = Et, sBu) in THF at room temperature. The isolation of 9 and its inertness toward cyclic ethers might be attributed to the higher electronegativity of gallium versus aluminum. The stronger Ga(I)-H bond, in turn, hampers α-C-H metalation

  14. Active use of urban park facilities

    DEFF Research Database (Denmark)

    Lindberg, Michael; Schipperijn, Jasper

    2015-01-01

    Abstract Urban green spaces (UGS), and more specific a higher number of facilities in UGS, have been positively associated with physical activity (PA). However, more detailed studies of which facilities generate high levels of PA, for which type of users, are relevant as existing knowledge...

  15. 7 CFR 58.127 - Facilities.

    Science.gov (United States)

    2010-01-01

    ... by the local health officer. The results of all water tests shall be kept on file at the plant for... regulations of the appropriate agency. (b) Drinking-water facilities. Drinking-water facilities of a sanitary... of 21 CFR 173.310 shall be used, or a secondary steam generator shall be used in which soft water...

  16. Jupiter Laser Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Jupiter Laser Facility is an institutional user facility in the Physical and Life Sciences Directorate at LLNL. The facility is designed to provide a high degree...

  17. Basic Research Firing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Basic Research Firing Facility is an indoor ballistic test facility that has recently transitioned from a customer-based facility to a dedicated basic research...

  18. Comparative in vitro activities of seven new beta-lactams, alone and in combination with beta-lactamase inhibitors, against clinical isolates resistant to third generation cephalosporins

    Directory of Open Access Journals (Sweden)

    Varsha Gupta

    Full Text Available We examined the drug susceptibility pattern of Gram-negative bacilli to seven new beta-lactams. A total of 277 non-duplicate gramnegative bacilli strains belonging to the Enterobacteriaceae family, Pseudomonas and Acinetobacter species, isolated from various clinical samples were tested for susceptibility to imipenem, piperacillin/tazobactam, cefoperazone/sulbactam, ticarcillin/clavulanate, cefdinir, cefepime and cefpirome with the disk diffusion technique. The percentage resistance was low for imipenem (7.2%, piperacillin/tazobactam (2.8%, cefoperazone/sulbactam (5.4%. However, a high frequency of resistance was observed to ticarcillin/clavulanate (83.9%, cefdinir (70.6%, cefepime (45.5% and cefpirome (84.4%. We conclude that imipenem, piperacillin/tazobactam and cefoperazone/sulbactam are effective antibiotics in our environment, whereas ticarcillin/clavulanate, cefdinir, cefepime and cefpirome are relatively uneffective.

  19. The generation of electric power in a model of endogenous development: possible solutions for isolated communities in the Amazonas State, Brazil; A geracao de energia eletrica em um modelo de desenvolvimento endogeno: possiveis solucoes para as comunidades isoladas do interior do estado do Amazonas

    Energy Technology Data Exchange (ETDEWEB)

    Teixeira, Andre Frazao; Cavaliero, Carla Kazue Nakao [Universidade Estadual de Campinas (FEM/UNICAMP), SP (Brazil). Fac. de Engenharia Mecanica

    2006-07-01

    This work was developed to fit the generation of electric energy for the isolated communities of the interior of the Amazonas State in a context of endogenous development. A regional development is proposed, with the inclusion of the generation of electric energy for these communities in a context of development, rather than the isolated form proposed in the federal government electrification program of 'Luz Para Todos' (Light For All). (author)

  20. Complete genome sequence of Corynebacterium variabile DSM 44702 isolated from the surface of smear-ripened cheeses and insights into cheese ripening and flavor generation

    Science.gov (United States)

    2011-01-01

    Background Corynebacterium variabile is part of the complex microflora on the surface of smear-ripened cheeses and contributes to the development of flavor and textural properties during cheese ripening. Still little is known about the metabolic processes and microbial interactions during the production of smear-ripened cheeses. Therefore, the gene repertoire contributing to the lifestyle of the cheese isolate C. variabile DSM 44702 was deduced from the complete genome sequence to get a better understanding of this industrial process. Results The chromosome of C. variabile DSM 44702 is composed of 3, 433, 007 bp and contains 3, 071 protein-coding regions. A comparative analysis of this gene repertoire with that of other corynebacteria detected 1, 534 predicted genes to be specific for the cheese isolate. These genes might contribute to distinct metabolic capabilities of C. variabile, as several of them are associated with metabolic functions in cheese habitats by playing roles in the utilization of alternative carbon and sulphur sources, in amino acid metabolism, and fatty acid degradation. Relevant C. variabile genes confer the capability to catabolize gluconate, lactate, propionate, taurine, and gamma-aminobutyric acid and to utilize external caseins. In addition, C. variabile is equipped with several siderophore biosynthesis gene clusters for iron acquisition and an exceptional repertoire of AraC-regulated iron uptake systems. Moreover, C. variabile can produce acetoin, butanediol, and methanethiol, which are important flavor compounds in smear-ripened cheeses. Conclusions The genome sequence of C. variabile provides detailed insights into the distinct metabolic features of this bacterium, implying a strong adaption to the iron-depleted cheese surface habitat. By combining in silico data obtained from the genome annotation with previous experimental knowledge, occasional observations on genes that are involved in the complex metabolic capacity of C. variabile

  1. Model of electric power generation for isolated places of the Amazon: intervention with sustainability; Modelo de geracao de energia eletrica para localidades isoladas da Amazonia: intervencao com sustentabilidade

    Energy Technology Data Exchange (ETDEWEB)

    Moret, Artur de Souza [Fundacao Universidade Federal de Rondonia (GPERS/UNIR), Porto Velho (Brazil). Dept. de Fisica. Grupo de Pesquisa Energia Renovavel Sustentavel], E-mail: amoret@unir.br

    2010-07-01

    This paper presents a proposal which has as reference the implementation of electric power generation using vegetable in natura palm oil in a Diesel cycle engine not modified to attend the N. S. dos Seringueiros at the extractivist reservation Rio Ouro Preto, Brazil. It must be detached that the interventions at the RESEX has assumed that it is possible and necessary to aggregate value to the forest products having the biodiversity preserved.

  2. Generation of an infectious clone of VR-2332, a highly virulent North American type isolate of porcine reproductive and respiratory syndrome virus

    DEFF Research Database (Denmark)

    Nielsen, H.S.; Liu, G.; Nielsen, Jens

    2003-01-01

    -2332 strain. However, the cloned virus was clearly distinguishable from the parental VR-2332 strain by an engineered marker, a BstZ171 restriction site. The full-length cDNA clone had 11 nucleotide changes, 2 of which affected coding, compared to the parental VR-2332 strain. Additionally...... mechanisms behind PRRSV virulence and attenuation, which might in turn allow the production of second-generation, genetically engineered PRRSV vaccines....

  3. Electric power generation using biomass gasification systems in nature in isolated communities of the Amazon region: project GASEIBRAS; Geracao de eletricidade utilizando sistemas de gaseificacao de biomassa in natura em comunidades isoladas da regiao amazonica: projeto GASEIBRAS

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira; Velazquez, Silvia M. Stortini Goncalves; Santos, Sandra M. Apolinario dos; Lora, Beatriz Acquaro [Universidade de Sao Paulo (CENBIO/USP), SP (Brazil). Centro de Referencia Nacional em Biomassa], e-mails: suani@iee.usp.br, sgvelaz@iee.usp.br, sandra@iee.usp.br, blora@iee.usp.br

    2006-07-01

    This paper will present the pioneering project of electric energy generation from renewable sources 'GASEIBRAS - Nationalization of the Biomass Gasification Technology and Formation of Human Resources in the Amazon Region', recently approved by the National Advice of Scientific and Technological Development (CNPq) and for the Ministry of Mines and Energy (MME). The GASEIBRAS project intends to use the experience previously acquired in the project GASEIFAMAZ - Comparison between Existing Technologies of Biomass Gasification in Brazil and Exterior and Formation of Human Resources in the North Region, sponsored by FINEP/CTENERG, to develop and construct a 20 kWe biomass gasification system, with total national technology, easy to operate and to maintain, and fed with local available biomass residues. Apart from contributing for the development of the national technology, this project will provide the sustainable development of the isolated communities in the Amazon region. The ongoing development of this project will enable to consolidate the national biomass gasification technology for electricity generation. The implemented prototype will allow the response of this project in other regions of the country, due its tailor made characteristic to attend to small isolated communities, thus supplying decentralized energy from renewable sources, to Amazon region. (author)

  4. Facility Registry Service (FRS)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Facility Registry Service (FRS) provides an integrated source of comprehensive (air, water, and waste) environmental information about facilities across EPA,...

  5. Licensed Healthcare Facilities

    Data.gov (United States)

    California Department of Resources — The Licensed Healthcare Facilities point layer represents the locations of all healthcare facilities licensed by the State of California, Department of Health...

  6. High Throughput Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Argonne?s high throughput facility provides highly automated and parallel approaches to material and materials chemistry development. The facility allows scientists...

  7. Aperture area measurement facility

    Data.gov (United States)

    Federal Laboratory Consortium — NIST has established an absolute aperture area measurement facility for circular and near-circular apertures use in radiometric instruments. The facility consists of...

  8. Environmental Toxicology Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Fully-equipped facilities for environmental toxicology research The Environmental Toxicology Research Facility (ETRF) located in Vicksburg, MS provides over 8,200 ft...

  9. Licensed Healthcare Facilities

    Data.gov (United States)

    California Department of Resources — The Licensed Healthcare Facilities point layer represents the locations of all healthcare facilities licensed by the State of California, Department of Health...

  10. Development of base isolation system for earthquakes and micro-vibrations using laminated thick rubber bearings. Part 3. ; Application to a medical facility building subjected to railway-induced vibration. Atusniku sekiso gum wo mochiita senshinter dot joshin system no kaihatsu. 3. ; Densha shindo ni taisuru sentan iryo shisetsu no joshin

    Energy Technology Data Exchange (ETDEWEB)

    Teramura, A.; Yoshihara, J.; Nakamura, M.; Ishikawa, R.; Yasui, Y.; Suzuki, T.; Okada, H (Obayashi Corp., Tokyo (Japan))

    1991-08-10

    A base isolation system using laminated thick rubber bearings was developed aiming for isolation of not only earthquake vibrations, but also railway-induced micro-vibrations. This system was applied to a medical facility building in which several sets of high-technology equipment, such as a medical small cyclotron and a position emitting computed tomography, were installed. The building was constructed at a site adjacent to a railway. In the design of the base isolation system for earthquakes, the dynamic-response analysis was done for recorded and simulated vibrations of earthquakes. For the micro-vibrations, effects of the railway-induced vibration on the base of the site were surveyed beforehand, and then the extensive base isolation system including foundation was investigated, in which an application of laminated thick rubber bearings and development of effective dampers to vertical vibrations were important subjects. Measurements done for the railway-induced micro-vibrations when the building frame was completed, showed that the ground surface vibration at a level from 40 to 50 gal was reduced toa level less than 1 gal on the building floor, and the appropriateness of the design technique and the effectiveness of the system against micro-vibrations were confirmed. 5 refs., 20 figs., 3 tabs.

  11. Tandem mirror technology demonstration facility

    Energy Technology Data Exchange (ETDEWEB)

    1983-10-01

    This report describes a facility for generating engineering data on the nuclear technologies needed to build an engineering test reactor (ETR). The facility, based on a tandem mirror operating in the Kelley mode, could be used to produce a high neutron flux (1.4 MW/M/sup 2/) on an 8-m/sup 2/ test area for testing fusion blankets. Runs of more than 100 h, with an average availability of 30%, would produce a fluence of 5 mW/yr/m/sup 2/ and give the necessary experience for successful operation of an ETR.

  12. Impact of Photovoltaic Generation on Reliability of Small Isolated PV-Wind-Diesel-Battery Power Generation System%光伏发电对风光柴储小型孤立发电系统可靠性的影响

    Institute of Scientific and Technical Information of China (English)

    王凯; 栗文义; 张晓飞; 白新雷

    2013-01-01

    In view of complementary features between resources of wind energy and solar energy among seasons and between day and night, small isolated wind-PV-diesel-battery power generation systems can supply power for remote areas better. A reliability assessment model of power output and solar energy radiant quantity, in which the variation of inclination angle of photovoltaic (PV) cell panel is reflected, is established. Utilizing the well-being model based Monte Carlo simulation the reliability of small isolated wind-PV-diesel-battery power generation system is assessed. Based on the results from calculation example, the impacts of the inclination angle of PV cell panel, peak power output of PV generation and forced outage on the reliability of the small isolated power generation system, and the obtained results are available for reference to the planning, design and operation of small isolated wind-PV-diesel-battery power generation systems.%因风能和太阳能资源具有季节和昼夜上的互补特性,风光柴储小型孤立发电系统可较好地为偏远地区提供电力服务。建立了反映光伏电池板倾斜角变化的太阳能辐射量及功率输出的可靠性评估模型。采用基于well-being模型的蒙特卡罗模拟法评估了风光柴储小型孤立发电系统可靠性。结合算例分析了光伏电池板倾角、光伏发电峰值功率及强迫停运率等因素对系统可靠性的影响。结果可为风光柴储小型孤立发电系统规划、设计和运行提供指导和参考。

  13. Guide to research facilities

    Energy Technology Data Exchange (ETDEWEB)

    1993-06-01

    This Guide provides information on facilities at US Department of Energy (DOE) and other government laboratories that focus on research and development of energy efficiency and renewable energy technologies. These laboratories have opened these facilities to outside users within the scientific community to encourage cooperation between the laboratories and the private sector. The Guide features two types of facilities: designated user facilities and other research facilities. Designated user facilities are one-of-a-kind DOE facilities that are staffed by personnel with unparalleled expertise and that contain sophisticated equipment. Other research facilities are facilities at DOE and other government laboratories that provide sophisticated equipment, testing areas, or processes that may not be available at private facilities. Each facility listing includes the name and phone number of someone you can call for more information.

  14. Perspective for the generation of electrical energy in isolated communities in the Amazon: a case study; Perspectivas para a geracao de energia eletrica em comunidades isoladas na Amazonia: um estudo de caso

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez Velazquez, Silvia Maria Stortini; Santos, Sandra Maria Apolinario; Moreira, Jose Roberto; Coelho, Suani Teixeira [Universidade Presbiteriana Mackenzie, Sao Paulo, SP (Brazil); Centro Nacional de Referencia em Biomassa (CENBIO), Sao Paulo, SP (Brazil)

    2010-07-01

    Valuing men and the environment in the developing process is essential due to the main model nowadays, which does not harmonize economical development and environmental preservation, besides wildly exploring the natural resources, degrading the environment and concentrating income, and being socially excluding. There is the need to create a model developed harmonically with the environment, focusing the attention on the social and environmental effects caused by the human activities and on the actions that must be taken aiming at the sustainability, the overcome of the social exclusion, and human development. This essay presents a Case Study focused on the electricity generation in an isolated community, which was developed due to the social and environmental matters. The isolated communities are located on the Brazilian North and Northeast regions, which concentrate most of the Brazilian communities with no access to the electric energy distribution network, which is the electricity supply model in Brazil. The low population density regions, like the Amazon region, present low indices of electrification because of this model, since the extension of the network for attending few consumers, is usually economically and environmentally impracticable. The insertion of an energy source of local occurrence, in this case, residues from the forest activities, will bring environmental benefits associated to the social benefits. The energy generation linked to economic activities will generate jobs and income, besides promoting the fossil fuels substitution, decreasing the CO{sub 2} emissions into the atmosphere and the environmental impact caused by the inadequate disposal of the residues, which are many times, burned in the open skies or disposed on the rivers. (author)

  15. Isolation and spectral characterization of thermally generated multi-Z-isomers of lycopene and the theoretically preferred pathway to di-Z-isomers.

    Science.gov (United States)

    Honda, Masaki; Kudo, Tatsuya; Kuwa, Takahiro; Higashiura, Takuma; Fukaya, Tetsuya; Inoue, Yoshinori; Kitamura, Chitoshi; Takehara, Munenori

    2017-02-01

    Lycopene has a large number of geometric isomers caused by E/Z isomerization at arbitrary sites within the 11 conjugated double bonds, offering varying characteristics related to features such as antioxidant capacity and bioavailability. However, the geometric structures of only a few lycopene Z-isomers have been thoroughly identified from natural sources. In this study, seven multi-Z-isomers of lycopene, (9Z,13'Z)-, (5Z,13Z,9'Z)-, (9Z,9'Z)-, (5Z,13'Z)-, (5Z,9'Z)-, (5Z,9Z,5'Z)-, and (5Z,9Z)-lycopene, were obtained from tomato samples by thermal isomerization, and then isolated by elaborate chromatography, and fully assigned using proton nuclear magnetic resonance. Moreover, the theoretically preferred pathway from (all-E)-lycopene to di-Z-isomers was examined with a computational approach using a Gaussian program. Fine-tuning of the HPLC separation conditions led to the discovery of novel multi-Z-isomers, and whose formation was supported by advanced theoretical calculations.

  16. Isolation and characteristics of one marine psychrotrophic cellulase-generating bacterium Ar/w/b/75°/10/5 from Chuckchi Sea, Arctic

    Institute of Scientific and Technical Information of China (English)

    曾胤新; 陈波

    2002-01-01

    Microorganisms living in polar zones play an important part as the potential source of organic activity materials with low temperature characteristics in the biotechnological applications. A psychrotrophic bacterium (strain Ar/w/b/75°/10/5), producing cellulase at low temperatures during late-exponential and early-stationary phases of cell growth, was isolated from sea ice-covered surface water in Chuckchi Sea, Arctic. This bacterium, with rod cells, was Gram-negative, slightly halophilic. Colony growing on agar plate was in black. Optimum growth temperature was 15℃. No cell growth was observed at 35℃ or above. Optimum salt concentration for cell growth was between 2 and 3 % of sodium chloride in media. Maximal cellulase activity was detected at a temperature of 35℃ and pH8. Cellulase was irreversibly inactivated when incubated at 55℃ within 30 min. Enzyme can be kept stable at the temperature no higher than 25℃. Of special interest was that this bacterium produced various extracellular enzymes including cellulase, amylase, agar hydrolase and protease, at low or moderate temperature conditions, which is certainly of it potential value for applications.

  17. Novel Halomonas sp. B15 isolated from Larnaca Salt Lake in Cyprus that generates vanillin and vanillic acid from ferulic acid.

    Science.gov (United States)

    Vyrides, Ioannis; Agathangelou, Maria; Dimitriou, Rodothea; Souroullas, Konstantinos; Salamex, Anastasia; Ioannou, Aristostodimos; Koutinas, Michalis

    2015-08-01

    Vanillin is a high value added product with many applications in the food, fragrance and pharmaceutical industries. A natural and low-cost method to produce vanillin is by microbial bioconversions through ferulic acid. Until now, limited microorganisms have been found capable of bioconverting ferulic acid to vanillin at high yield. This study aimed to screen halotolerant strains of bacteria from Larnaca Salt Lake which generate vanillin and vanillic acid from ferulic acid. From a total of 50 halotolenant/halophilic strains 8 grew in 1 g/L ferulic acid and only 1 Halomonas sp. B15 and 3 Halomonas elognata strains were capable of bioconverting ferulic acid to vanillic acid at 100 g NaCl/L. The highest vanillic acid (365 mg/L) at these conditions generated by Halomonas sp. B15 which corresponds to ferulic acid bioconversion yield of 36.5%. Using the resting cell technique with an initial ferulic acid concentration of 0.5 g/L at low salinity, the highest production of vanillin (245 mg/L) took place after 48 h, corresponding to a bioconversion yield of 49%. This is the first reported Halomonas sp. with high yield of vanillin production from ferulic acid at low salinity.

  18. Cross-resistance profile determination of two second-generation HIV-1 integrase inhibitors using a panel of recombinant viruses derived from raltegravir-treated clinical isolates.

    Science.gov (United States)

    Van Wesenbeeck, L; Rondelez, E; Feyaerts, M; Verheyen, A; Van der Borght, K; Smits, V; Cleybergh, C; De Wolf, H; Van Baelen, K; Stuyver, L J

    2011-01-01

    The integrase inhibitor raltegravir (RAL) is currently used for the treatment of both treatment-naïve and treatment-experienced HIV-1-infected patients. Elvitegravir (EVG) is in late phases of clinical development. Since significant cross-resistance between RAL and EVG is observed, there is a need for second-generation integrase inhibitors (INIs) with a higher genetic barrier and limited cross-resistance to RAL/EVG. A panel of HIV-1 integrase recombinants, derived from plasma samples from raltegravir-treated patients (baseline and follow-up samples), were used to study the cross-resistance profile of two second-generation integrase inhibitors, MK-2048 and compound G. Samples with Q148H/R mutations had elevated fold change values with all compounds tested. Although samples with the Y143R/C mutation had reduced susceptibility to RAL, they remained susceptible to MK-2048 and compound G. Samples with the N155H mutation had no reduced susceptibility to compound G. In conclusion, our results allowed ranking of the INIs on the basis of the antiviral activities using recombinant virus stocks from RAL-treated patient viruses. The order according to decreasing susceptibility is compound G, MK-2048, and EVG.

  19. Comparative analysis between two systems to generate electric energy for isolated community in the interior of the Amazon state: fuel cells with natural gas reformer versus diesel generation; Analise comparativa entre dois sistemas de geracao de energia eletrica para a comunidade isolada no interior do estado do Amazonas: celula a combustivel com reformador para gas natural versus gerador diesel

    Energy Technology Data Exchange (ETDEWEB)

    Araujo, Paula Duarte; Bergamini, Cristiane Peres; Camargo, Joao Carlos; Lopes, Daniel Gabriel [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Fac. de Engenharia Mecanica; Esteves, Gheisa Roberta Telles [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Nucleo de Pesquisas e Estudos Ambientais; Silva, Ennio Peres da Silva [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Inst. de Fisica Gleb Wataghin

    2004-07-01

    Although great part of the domestic territory is already supplied with electric energy, still there are many regions where the system is precarious or nonexistent, generically called isolated communities. In the majority of the cases these communities are supplied with Diesel oil generators and the substitution of this fuel for available alternative energy in the localities has been object of study of some institutions of research spread throughout the country. Currently, the use of fuel cells has been strongly argued in the generation of electric energy associated with the local energy necessity, from the use of a regional fuel and this is due to the high efficiency of allied energy conversion to the low ambient impacts that this equipment offers. Most of the different types of fuel cells use hydrogen as a fuel to produce electricity, and it is extracted from renewable or non-renewable sources of energy. Then, the article has the objective of comparing in first analysis the energy efficiency and the cost between the two systems: the ones used currently in the great majority of the isolated communities, constituted of a Diesel engine-generator system, with Natural Gas Reformer System/ Purifier of Hydrogen/ Fuel Cell/ and to analyze if such project presents characteristics that qualifies it to get the carbon credits proposed in the Mechanism of Clean Development. (author)

  20. Multidrug-Resistant Salmonella Heidelberg Associated with Mechanically Separated Chicken at a Correctional Facility.

    Science.gov (United States)

    Taylor, Amanda L; Murphree, Rendi; Ingram, L Amanda; Garman, Katie; Solomon, Deborah; Coffey, Eric; Walker, Deborah; Rogers, Marsha; Marder, Ellyn; Bottomley, Marie; Woron, Amy; Thomas, Linda; Roberts, Sheri; Hardin, Henrietta; Arjmandi, Parvin; Green, Alice; Simmons, Latoya; Cornell, Allyson; Dunn, John

    2015-12-01

    We describe multidrug-resistant (MDR) Salmonella Heidelberg infections associated with mechanically separated chicken (MSC) served at a county correctional facility. Twenty-three inmates met the case definition. All reported diarrhea, 19 (83%) reported fever, 16 (70%) reported vomiting, 4 (17%) had fever ≥103°F, and 3 (13%) were hospitalized. A case-control study found no single food item significantly associated with illness. Salmonella Heidelberg with an indistinguishable pulsed-field gel electrophoresis pattern was isolated from nine stool specimens; two isolates displayed resistance to a total of five drug classes, including the third-generation cephalosporin, ceftriaxone. MDR Salmonella Heidelberg might have contributed to the severity of illness. Salmonella Heidelberg indistinguishable from the outbreak subtype was isolated from unopened MSC. The environmental health assessment identified cross-contamination through poor food-handling practices as a possible contributing factor. Proper hand-washing techniques and safe food-handling practices were reviewed with the kitchen supervisor.

  1. Geothermal energy conversion facility

    Energy Technology Data Exchange (ETDEWEB)

    Kutscher, C.F.

    1997-12-31

    With the termination of favorable electricity generation pricing policies, the geothermal industry is exploring ways to improve the efficiency of existing plants and make them more cost-competitive with natural gas. The Geothermal Energy Conversion Facility (GECF) at NREL will allow researchers to study various means for increasing the thermodynamic efficiency of binary cycle geothermal plants. This work has received considerable support from the US geothermal industry and will be done in collaboration with industry members and utilities. The GECF is being constructed on NREL property at the top of South Table Mountain in Golden, Colorado. As shown in Figure 1, it consists of an electrically heated hot water loop that provides heating to a heater/vaporizer in which the working fluid vaporizes at supercritical or subcritical pressures as high as 700 psia. Both an air-cooled and water-cooled condenser will be available for condensing the working fluid. In order to minimize construction costs, available equipment from the similar INEL Heat Cycle Research Facility is being utilized.

  2. Manassantin B isolated from Saururus chinensis inhibits cyclooxygenase-2-dependent prostaglandin D2 generation by blocking Fyn-mediated nuclear factor-kappaB and mitogen activated protein kinase pathways in bone marrow derived-mast cells.

    Science.gov (United States)

    Lu, Yue; Hwang, Seung-Lark; Son, Jong Keun; Chang, Hyeun Wook

    2013-01-01

    The authors investigated the effect of manassantin B (Man B) isolated from Saururus chinensis (S. chinensis) on cyclooxygenase-2 (COX-2)-dependent prostaglandin D2 (PGD2) generation in mouse bone marrow derived-mast cells (BMMCs). Man B inhibited the generation of PGD2 dose-dependently by inhibiting COX-2 expression in immunoglobulin E (IgE)/Ag-stimulated BMMCs. To elucidate the mechanism responsible for the inhibition of COX-2 expression by Man B, the effects of Man B on the activation of nuclear factor-kappaB (NF-κB), a transcription factor essential and mitogen-activated protein kinases (MAPKs) for COX-2 induction, were examined. Man B attenuated the nuclear translocation of NF-κB p65 and its DNA-binding activity by inhibiting inhibitors of kappa Bα (IκBα) degradation and concomitantly suppressing IκB kinase (IKK) phosphorylation. In addition, Man B suppressed phosphorylation of MAPKs including extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun NH2-terminal kinase (JNK) and p38. It was also found that Man B suppressed Fyn kinase activation and consequent downstream signaling processes, including those involving Syk, Gab2, and Akt. Taken together, the present results suggest that Man B suppresses COX-2 dependent PGD2 generation by primarily inhibiting Fyn kinase in FcεRI-mediated mast cells.

  3. National Ignition Facility site requirements

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-07-01

    The Site Requirements (SR) provide bases for identification of candidate host sites for the National Ignition Facility (NIF) and for the generation of data regarding potential actual locations for the facilities. The SR supplements the NIF Functional Requirements (FR) with information needed for preparation of responses to queries for input to HQ DOE site evaluation. The queries are to include both documents and explicit requirements for the potential host site responses. The Sr includes information extracted from the NIF FR (for convenience), data based on design approaches, and needs for physical and organization infrastructure for a fully operational NIF. The FR and SR describe requirements that may require new construction or may be met by use or modification of existing facilities. The SR do not establish requirements for NIF design or construction project planning. The SR document does not constitute an element of the NIF technical baseline.

  4. Power Systems Development Facility

    Energy Technology Data Exchange (ETDEWEB)

    Southern Company Services

    2009-01-31

    In support of technology development to utilize coal for efficient, affordable, and environmentally clean power generation, the Power Systems Development Facility (PSDF), located in Wilsonville, Alabama, has routinely demonstrated gasification technologies using various types of coals. The PSDF is an engineering scale demonstration of key features of advanced coal-fired power systems, including a Transport Gasifier, a hot gas particulate control device, advanced syngas cleanup systems, and high-pressure solids handling systems. This final report summarizes the results of the technology development work conducted at the PSDF through January 31, 2009. Twenty-one major gasification test campaigns were completed, for a total of more than 11,000 hours of gasification operation. This operational experience has led to significant advancements in gasification technologies.

  5. Reliable Facility Location Problem with Facility Protection.

    Science.gov (United States)

    Tang, Luohao; Zhu, Cheng; Lin, Zaili; Shi, Jianmai; Zhang, Weiming

    2016-01-01

    This paper studies a reliable facility location problem with facility protection that aims to hedge against random facility disruptions by both strategically protecting some facilities and using backup facilities for the demands. An Integer Programming model is proposed for this problem, in which the failure probabilities of facilities are site-specific. A solution approach combining Lagrangian Relaxation and local search is proposed and is demonstrated to be both effective and efficient based on computational experiments on random numerical examples with 49, 88, 150 and 263 nodes in the network. A real case study for a 100-city network in Hunan province, China, is presented, based on which the properties of the model are discussed and some managerial insights are analyzed.

  6. Status of Myrrha and ISOL@MYRRHA in March 2010

    Science.gov (United States)

    Abderrahim, H. Aït; Heyse, J.; Wagemans, J.

    2011-10-01

    From 1998 on, SCK•CEN has been studying the coupling of a proton accelerator, a liquid Lead-Bismuth Eutectic (LBE) spallation target and a LBE cooled, sub-critical fast reactor. The project, called MYRRHA, aims at constructing an Accelerator Driven System (ADS) at the SCK•CEN site in Mol (Belgium). MYRRHA is designed as a multipurpose irradiation facility in order to support research programmes on fission and fusion reactor structural materials and nuclear fuel development. Applications of these are found in ADS systems and in present generation as well as next generation critical reactors. The first objective of MYRRHA however will be to demonstrate on one hand the ADS concept at a reasonable power level and on the other hand the technological feasibility of transmutation of Minor Actinides (MA) and Long-Lived Fission products (LLFP) arising from the reprocessing of used fuel. MYRRHA will also help the development of the Pb alloys technology needed for the lead fast reactor Generation IV concept. Moreover, the implementation of this project will trigger the development of various applications, like a facility for fundamental physics at high-intensity Radioactive Ion Beams (RIB) called ISOL@MYRRHA. This paper presents the present status of the MYRRHA project and in particular the characteristics of ISOL@MYRRHA.

  7. Security culture for nuclear facilities

    Science.gov (United States)

    Gupta, Deeksha; Bajramovic, Edita

    2017-01-01

    Natural radioactive elements are part of our environment and radioactivity is a natural phenomenon. There are numerous beneficial applications of radioactive elements (radioisotopes) and radiation, starting from power generation to usages in medical, industrial and agriculture applications. But the risk of radiation exposure is always attached to operational workers, the public and the environment. Hence, this risk has to be assessed and controlled. The main goal of safety and security measures is to protect human life, health, and the environment. Currently, nuclear security considerations became essential along with nuclear safety as nuclear facilities are facing rapidly increase in cybersecurity risks. Therefore, prevention and adequate protection of nuclear facilities from cyberattacks is the major task. Historically, nuclear safety is well defined by IAEA guidelines while nuclear security is just gradually being addressed by some new guidance, especially the IAEA Nuclear Security Series (NSS), IEC 62645 and some national regulations. At the overall level, IAEA NSS 7 describes nuclear security as deterrence and detection of, and response to, theft, sabotage, unauthorized access, illegal transfer or other malicious acts involving nuclear, other radioactive substances and their associated facilities. Nuclear security should be included throughout nuclear facilities. Proper implementation of a nuclear security culture leads to staff vigilance and a high level of security posture. Nuclear security also depends on policy makers, regulators, managers, individual employees and members of public. Therefore, proper education and security awareness are essential in keeping nuclear facilities safe and secure.

  8. Implementation and testing of the use of vegetable oils as fuel diesel generators in the Amazon isolated communities; Implantacao e testes de utilizacao de oleo vegetal como combustivel para diesel geradores em comunidades isoladas da Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Coelho, Suani Teixeira; Silva, Orlando Cristiano da; Gonzalez Velaquez, Silvia Maria Stortini; Monteiro, Maria Beatriz C.A.; Silotto, Carlos Eduardo Grassi [Centro Nacional de Referencia em Biomassa (CENBIO), Sao Paulo, SP (Brazil)

    2004-07-01

    The project PROVEGAM 'Implantation and test of a unit demonstration of energetic utilization of vegetable oil', tested in operational conditions of field, the functioning of a conventional diesel engine, adapted to operate with palm oil 'in natura' in the community of Vila Soledade, city of Moju, Para State. The Vila Soledade is an isolated community that has, approximately, 700 inhabitants and it's located at one hundred kilometers from the city hall by car and more 30 minutes by boat. The electric energy of this community was previously generated, by a conventional diesel engine, obsolete and very expensive to the community, because the fuel price and the transport of the diesel oil from the city to the community. The PROVEGAM project, installed an electric generation group, MWM TD229, manufactured in Brazil, adapted with a conversion kit to operate with 'in natura' palm oil, working 6 hours per day. Because of the viscosity of the palm oil and its combustion point, it was necessary to heat the vegetable oil before its injection into the engine. The operation begins and finishes with diesel oil, in order to heat the palm oil and to clean possible residues deposited in the interior of the engine. The use of the palm oil justifies itself for being produced in the region, which means that it doesn't have to be imported. Currently, the generating group is working in the community during 5 hours per day with palm oil, and 1 hour per day with diesel oil and it already has more than 1600 hours of testing. The results of this project, so far, have confirmed the conceived premises, and this electric model of generating energy is already recommended to be implemented in other communities in the Amazon region. (author)

  9. Sizing an isolated wind-solar-fuel cell generation system based on the particle swarm optimization method; Dimensionamiento de un sistema de generacion aislado eolico-solar-celda de combustible basado en el metodo de optimizacion de enjambre de particulas

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez-Huerta, V; Ramirez-Arredondo, Juan M. [Universidad de Quintana Roo, Chetumal, Quintana Roo (Mexico)]. E-mail: vsanchez@gdl.cinvestav.mx; Arriaga-Hurtado, L. G. [CIDETEQ, Queretaro (Mexico)

    2009-09-15

    Sizing an electric energy system requires an analysis of investment, maintenance and operating costs. In the case of a generation system that uses renewable sources, optimal capacity becomes more complex compared to a conventional system, because of the randomness of renewable resources (wind, solar) and the still high costs of wind and photovoltage generator modules. This work presents the optimal sizing of a wind-solar-fuel cell generation system, minimizing the costs of the system while satisfying the energy demands of an isolated charge. The optimization method used is based on an evolutionary programming technique known as particle swarms (PSO-particle swarm optimization). The generation of energy with a hybrid system is discussed, based on the profile of insolation and wind availability at the site, with the objective of satisfying a specific electric demand. [Spanish] El dimensionamiento de un sistema de generacion de energia electrica requiere un analisis de los costos de inversion, mantenimiento y operacion. En el caso de un sistema de generacion que utiliza fuentes renovables la capacidad optima resulta mas compleja con respecto a un sistema convencional, debido a la aleatoriedad de los recursos renovables (eolico, solar), y a los aun altos costos de generadores eolicos y modulos fotovoltaicos. En este trabajo se presenta el dimensionamiento optimo de un sistema de generacion eolico-solar-celda de combustible minimizando los costos del sistema que satisfaga la energia demandada por una carga aislada. El metodo de optimizacion utilizado esta basado en una tecnica de programacion evolutiva conocida como enjambre de particulas (PSO por sus siglas en ingles: particle swarm optimization). Se plantea la generacion de energia del sistema hibrido con base a la insolacion y el perfil del viento disponible en sitio, con objeto de satisfacer una demanda electrica determinada.

  10. Design and construction of thermal striping test facility

    Energy Technology Data Exchange (ETDEWEB)

    Han, D. H.; Kim, J. M.; Nam, H. Y.; Choi, J. H.; Choi, B. H.; Jeong, J. Y.; Jeong, K. C.; Park, J. H.; Kim, T. J.; Kim, B. H

    2003-12-01

    Test facility was designed and constructed to generate of experimental data for the validation of turbulence model for analyzing thermal striping phenomena. Test facility consists mainly of test section, heat transfer system and control system. In this report design and construction process of test facility was described in detail.

  11. Steady State Vacuum Ultraviolet Exposure Facility With Automated Calibration Capability

    Science.gov (United States)

    Stueber, Thomas J.; Sechkar, Edward A.; Dever, Joyce A.; Banks, Bruce A.

    2000-01-01

    NASA Glenn Research Center at Lewis Field designed and developed a steady state vacuum ultraviolet automated (SSVUVa) facility with in situ VUV intensity calibration capability. The automated feature enables a constant accelerated VUV radiation exposure over long periods of testing without breaking vacuum. This test facility is designed to simultaneously accommodate four isolated radiation exposure tests within the SSVUVa vacuum chamber. Computer-control of the facility for long, term continuous operation also provides control and recording of thermocouple temperatures, periodic recording of VUV lamp intensity, and monitoring of vacuum facility status. This paper discusses the design and capabilities of the SSVUVa facility.

  12. High-level waste inventory, characteristics, generation, and facility assessment for treatment, storage, and disposal alternatives considered in the US Department of Energy eenvironmental management programmatic environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    Folga, S.M.; Conzelmann, G.; Gillette, J.L.; Kier, P.H.; Poch, L.A.

    1996-12-01

    This report provides data and information needed to support the risk and impact assessments of high-level waste (HLW) management alternatives in the U.S. Department of Energy Waste Management (WM) Programmatic Environmental Impact Statement (PEIS). Available data on the physical form, chemical and isotopic composition, storage locations, and other waste characteristics of interest are presented. High-level waste management follows six implementation phases: current storage, retrieval, pretreatment, treatment, interim canister storage, and geologic repository disposal; pretreatment, treatment, and repository disposal are outside the scope of the WM PEIS. Brief descriptions of current and planned HLW management facilities are provided, including information on the type of waste managed in the facility, costs, product form, resource requirements, emissions, and current and future status. Data sources and technical and regulatory assumptions are identified. The range of HLW management alternatives (including decentralized, regionalized, and centralized approaches) is described. The required waste management facilities include expanded interim storage facilities under the various alternatives. Resource requirements for construction (e.g., land and materials) and operation (e.g., energy and process chemicals), work force, costs, effluents, design capacities, and emissions are presented for each alternative.

  13. High-level waste inventory, characteristics, generation, and facility assessment for treatment, storage, and disposal alternatives considered in the US Department of Energy eenvironmental management programmatic environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    Folga, S.M.; Conzelmann, G.; Gillette, J.L.; Kier, P.H.; Poch, L.A.

    1996-12-01

    This report provides data and information needed to support the risk and impact assessments of high-level waste (HLW) management alternatives in the U.S. Department of Energy Waste Management (WM) Programmatic Environmental Impact Statement (PEIS). Available data on the physical form, chemical and isotopic composition, storage locations, and other waste characteristics of interest are presented. High-level waste management follows six implementation phases: current storage, retrieval, pretreatment, treatment, interim canister storage, and geologic repository disposal; pretreatment, treatment, and repository disposal are outside the scope of the WM PEIS. Brief descriptions of current and planned HLW management facilities are provided, including information on the type of waste managed in the facility, costs, product form, resource requirements, emissions, and current and future status. Data sources and technical and regulatory assumptions are identified. The range of HLW management alternatives (including decentralized, regionalized, and centralized approaches) is described. The required waste management facilities include expanded interim storage facilities under the various alternatives. Resource requirements for construction (e.g., land and materials) and operation (e.g., energy and process chemicals), work force, costs, effluents, design capacities, and emissions are presented for each alternative.

  14. Optimal design of a hybrid photovoltaic and fuel cell power generation system, to supply isolated communities in the Brazilian Amazon; Dimensionamento otimo de sistemas hibridos, com geracao fotovoltaica e celula a combustivel, para atendimento a comunidades isoladas na Amazonia

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Sergio Batista da

    2010-11-15

    The lack of electricity in isolated communities in the Brazilian Amazon has become one of the greatest barrier for the development of the region. Currently, the main technologies that provide electricity to these communities are diesel generators, batteries and dry cells. These non-renewable energy sources may pose serious problems to the environment and human health and have high maintenance and operational costs. Therefore, the search for renewable energy sources, such as water and sunlight, which are highly abundant in the region, has become a great challenge. This thesis presents the studies on application of solar photovoltaic (PV) and fuel cell (FC) technologies to supply electric power in an uninterrupted manner. Outlined are the technical and cost issues of a pilot project set up in an environmentally protected area, next to Bananal island, located in the Southwestern region of the state of Tocantins. The pilot project relies on PV solar power as the primary source of energy for the production of electricity. The surplus energy is stored in the form of hydrogen produced by electrolysis of the water supplied locally, which is reconverted into electric power by fuel cells during periods when there is little or no sunlight. In this context, the aim of the study was to propose a sizing of a hybrid distributed generation system (HDGS), comprised of a PV system, FC and batteries, that optimizes implementation and operational costs, as a potential source of energy for isolated communities in the Amazon. The work was carried out with the help of simulation software HOMER (Hybrid Optimization Model for Electric Renewable) developed by National Renewable Energy Laboratory (NREL). Simulations and a comparative study were carried out of the technologies and potential configurations that meet the needs of these isolated communities. The results showed an optimal solution of HGDS PV-FC batteries with a reduction in the initial cost of the project in about 60% compared to

  15. Coastal Energy Facilities in the United States for 2012.

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data depict the location of facilities that generate electricity. The locations are created from the Environmental Protection Agency Emissions & Generation...

  16. Narrowband NIR-Induced In Situ Generation of the High-Energy Trans Conformer of Trichloroacetic Acid Isolated in Solid Nitrogen and its Spontaneous Decay by Tunneling to the Low-Energy Cis Conformer

    Directory of Open Access Journals (Sweden)

    R. F. G. Apóstolo

    2015-12-01

    Full Text Available The monomeric form of trichloroacetic acid (CCl3COOH; TCA was isolated in a cryogenic nitrogen matrix (15 K and the higher energy trans conformer (O=C–O–H dihedral: 180° was generated in situ by narrowband near-infrared selective excitation the 1st OH stretching overtone of the low-energy cis conformer (O=C–O–H dihedral: 0°. The spontaneous decay, by tunneling, of the generated high-energy conformer into the cis form was then evaluated and compared with those observed previously for the trans conformers of acetic and formic acids in identical experimental conditions. The much faster decay of the high-energy conformer of TCA compared to both formic and acetic acids (by ~35 and ca. 25 times, respectively was found to correlate well with the lower energy barrier for the trans→cis isomerization in the studied compound. The experimental studies received support from quantum chemistry calculations undertaken at the DFT(B3LYP/cc-pVDZ level of approximation, which allowed a detailed characterization of the potential energy surface of the molecule and the detailed assignment of the infrared spectra of the two conformers.

  17. Multi-Purpose Thermal Hydraulic Loop: Advanced Reactor Technology Integral System Test (ARTIST) Facility for Support of Advanced Reactor Technologies

    Energy Technology Data Exchange (ETDEWEB)

    James E. O' Brien; Piyush Sabharwall; SuJong Yoon

    2001-11-01

    Effective and robust high temperature heat transfer systems are fundamental to the successful deployment of advanced reactors for both power generation and non-electric applications. Plant designs often include an intermediate heat transfer loop (IHTL) with heat exchangers at either end to deliver thermal energy to the application while providing isolation of the primary reactor system. In order to address technical feasibility concerns and challenges a new high-temperature multi-fluid, multi-loop test facility “Advanced Reactor Technology Integral System Test facility” (ARTIST) is under development at the Idaho National Laboratory. The facility will include three flow loops: high-temperature helium, molten salt, and steam/water. Details of some of the design aspects and challenges of this facility, which is currently in the conceptual design phase, are discussed

  18. Distributed generation induction and permanent magnet generators

    CERN Document Server

    Lai, L

    2007-01-01

    Distributed power generation is a technology that could help to enable efficient, renewable energy production both in the developed and developing world. It includes all use of small electric power generators, whether located on the utility system, at the site of a utility customer, or at an isolated site not connected to the power grid. Induction generators (IGs) are the cheapest and most commonly used technology, compatible with renewable energy resources. Permanent magnet (PM) generators have traditionally been avoided due to high fabrication costs; however, compared with IGs they are more reliable and productive. Distributed Generation thoroughly examines the principles, possibilities and limitations of creating energy with both IGs and PM generators. It takes an electrical engineering approach in the analysis and testing of these generators, and includes diagrams and extensive case study examples o better demonstrate how the integration of energy sources can be accomplished. The book also provides the ...

  19. Ouellette Thermal Test Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Thermal Test Facility is a joint Army/Navy state-of-the-art facility (8,100 ft2) that was designed to:Evaluate and characterize the effect of flame and thermal...

  20. Cold Vacuum Drying Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Located near the K-Basins (see K-Basins link) in Hanford's 100 Area is a facility called the Cold Vacuum Drying Facility (CVDF).Between 2000 and 2004, workers at the...

  1. Dialysis Facility Compare

    Data.gov (United States)

    U.S. Department of Health & Human Services — Dialysis Facility Compare helps you find detailed information about Medicare-certified dialysis facilities. You can compare the services and the quality of care that...

  2. Explosive Components Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The 98,000 square foot Explosive Components Facility (ECF) is a state-of-the-art facility that provides a full-range of chemical, material, and performance analysis...

  3. Materiel Evaluation Facility

    Data.gov (United States)

    Federal Laboratory Consortium — CRREL's Materiel Evaluation Facility (MEF) is a large cold-room facility that can be set up at temperatures ranging from −20°F to 120°F with a temperature change...

  4. Armament Technology Facility (ATF)

    Data.gov (United States)

    Federal Laboratory Consortium — The Armament Technology Facility is a 52,000 square foot, secure and environmentally-safe, integrated small arms and cannon caliber design and evaluation facility....

  5. Integrated Disposal Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Located near the center of the 586-square-mile Hanford Site is the Integrated Disposal Facility, also known as the IDF.This facility is a landfill similar in concept...

  6. Facilities for US Radioastronomy.

    Science.gov (United States)

    Thaddeus, Patrick

    1982-01-01

    Discusses major developments in radioastronomy since 1945. Topics include proposed facilities, very-long-baseline interferometric array, millimeter-wave telescope, submillimeter-wave telescope, and funding for radioastronomy facilities and projects. (JN)

  7. Wastewater Treatment Facilities

    Data.gov (United States)

    Iowa State University GIS Support and Research Facility — Individual permits for municipal, industrial, and semi-public wastewater treatment facilities in Iowa for the National Pollutant Discharge Elimination System (NPDES)...

  8. Facility Response Plan (FRP)

    Data.gov (United States)

    U.S. Environmental Protection Agency — A Facility Response Plan (FRP) demonstrates a facility's preparedness to respond to a worst case oil discharge. Under the Clean Water Act, as amended by the Oil...

  9. Financing Professional Sports Facilities

    OpenAIRE

    Baade, Robert A.; Victor A. Matheson

    2011-01-01

    This paper examines public financing of professional sports facilities with a focus on both early and recent developments in taxpayer subsidization of spectator sports. The paper explores both the magnitude and the sources of public funding for professional sports facilities.

  10. FDA Certified Mammography Facilities

    Science.gov (United States)

    ... Program Consumer Information (MQSA) Search for a Certified Facility Share Tweet Linkedin Pin it More sharing options ... Email Print This list of FDA Certified Mammography Facilities is updated weekly. If you click on Search ...

  11. Energetics Conditioning Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Energetics Conditioning Facility is used for long term and short term aging studies of energetic materials. The facility has 10 conditioning chambers of which 2...

  12. Energetics Conditioning Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Energetics Conditioning Facility is used for long term and short term aging studies of energetic materials. The facility has 10 conditioning chambers of which 2...

  13. Environmental Toxicology Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Fully-equipped facilities for environmental toxicology researchThe Environmental Toxicology Research Facility (ETRF) located in Vicksburg, MS provides over 8,200 ft...

  14. Ouellette Thermal Test Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Thermal Test Facility is a joint Army/Navy state-of-the-art facility (8,100 ft2) that was designed to: Evaluate and characterize the effect of flame and thermal...

  15. Projectile Demilitarization Facilities

    Data.gov (United States)

    Federal Laboratory Consortium — The Projectile Wash Out Facility is US Army Ammunition Peculiar Equipment (APE 1300). It is a pilot scale wash out facility that uses high pressure water and steam...

  16. AMRITA -- A computational facility

    Energy Technology Data Exchange (ETDEWEB)

    Shepherd, J.E. [California Inst. of Tech., CA (US); Quirk, J.J.

    1998-02-23

    Amrita is a software system for automating numerical investigations. The system is driven using its own powerful scripting language, Amrita, which facilitates both the composition and archiving of complete numerical investigations, as distinct from isolated computations. Once archived, an Amrita investigation can later be reproduced by any interested party, and not just the original investigator, for no cost other than the raw CPU time needed to parse the archived script. In fact, this entire lecture can be reconstructed in such a fashion. To do this, the script: constructs a number of shock-capturing schemes; runs a series of test problems, generates the plots shown; outputs the LATEX to typeset the notes; performs a myriad of behind-the-scenes tasks to glue everything together. Thus Amrita has all the characteristics of an operating system and should not be mistaken for a common-or-garden code.

  17. A feasibility and economic study into the use of micro hydro power applications to generate electricity from the Victoria Capital Regional District’s municipal water facility. Victoria, British Columbia, Canada.

    OpenAIRE

    Eri Andres Boye

    2011-01-01

    The Victoria Capitol Regional District (CRD) operates several pressure reducing facilities (PRF) as part of their potable municipal waterways. The Humpback PRF has the largest flow and head conditions, and was used as the study site in this thesis. A feasibility and economic study was conducted to observe the viability of a proposed hydropower station at the Humpback PRF. Feasibility analysis of flow and pressure: Flow and head conditions vary over the course of time, with lows in 2...

  18. NASA AURA HIRDLS instrument calibration facility

    Science.gov (United States)

    Hepplewhite, Christopher L.; Barnett, John J.; Watkins, Robert E. J.; Row, Frederick; Wolfenden, Roger; Djotni, Karim; Oduleye, Olusoji O.; Whitney, John G.; Walton, Trevor W.; Arter, Philip I.

    2003-11-01

    A state-of-the-art calibration facility was designed and built for the calibration of the HIRDLS instrument at the University of Oxford, England. This paper describes the main features of the facility, the driving requirements and a summary of the performance that was achieved during the calibration. Specific technical requirements and a summary of the performance that was achieved during the calibration. Specific technical requirements and other constaints determined the design solutions that were adopted and the implementation methodology. The main features of the facility included a high performance clean room, vacuum chamber with thermal environmental control as well as the calibration sources. Particular attention was paid to maintenance of cleanliness (molecular and particulate), ESD control, mechanical isolation and high reliability. Schedule constraints required that all the calibration sources were integrated into the facility so that the number of re-press and warm up cycles was minimized and so that all the equipment could be operated at the same time.

  19. Pressurized burner test facility

    Energy Technology Data Exchange (ETDEWEB)

    Maloney, D.J.; Norton, T.S.; Hadley, M.A. [Morgantown Energy Technology Center, WV (United States)

    1993-06-01

    The Morgantown Energy Technology Center (METC) is currently fabricating a high-pressure burner test facility. The facility was designed to support the development of gas turbine combustion systems fired on natural gas and coal-derived gaseous fuels containing fuel-bound nitrogen. Upon completion of fabrication and shake-down testing in October 1993, the facility will be available for use by industrial and university partners through Cooperative Research and Development Agreements (CRADAs) or through other cooperative arrangements. This paper describes the burner test facility and associated operating parameter ranges and informs interested parties of the availability of the facility.

  20. ESO VLT laser guide star facility

    Science.gov (United States)

    Bonaccini, Domenico; Hackenberg, Wolfgang K.; Cullum, Martin J.; Brunetto, Enzo; Ott, Thomas; Quattri, Marco; Allaert, Eric; Dimmler, Martin; Tarenghi, Massimo; Van Kersteren, A.; Di Chirico, C.; Buzzoni, Bernard; Gray, Peter; Tamai, R.; Tapia, M.

    2002-02-01

    We report in this paper on the design and progress of the ESO Laser Guide Star Facility. The project will create a user facility embedded in UT4, to produce in the Earth's Mesosphere Laser Guide Stars, which extend the sky coverage of Adaptive Optics systems on the VLT UT4 telescope. Embedded into the project are provisions for multiple LGS to cope with second generation MCAO instruments.

  1. Ukraine experimental neutron source facility.

    Energy Technology Data Exchange (ETDEWEB)

    Gohar, Y.; Bolshinsky, I.; Nekludov, I.; Karnaukhov, I. (Nuclear Engineering Division); (INL); (Kharkov Institute of Physics and Technology)

    2008-01-01

    Kharkov Institute of Physics and Technology (KIPT) of Ukraine has a plan to construct an experimental neutron source facility. The facility has been developed for producing medical isotopes, training young nuclear professionals, supporting the Ukraine nuclear industry, providing capability for performing reactor physics, material research, and basic science experiments. Argonne National Laboratory (ANL) of USA is collaborating with KIPT on developing this facility. A driven subcritical assembly utilizing the KIPT electron accelerator with a target assembly is used to generate the neutron source. The target assembly utilizes tungsten or uranium for neutron production through photonuclear reactions with 100-KW of electron beam power. The neutron source intensity, spectrum, and spatial distribution have been studied to maximize the neutron yield and satisfy different engineering requirements. The subcritical assembly is designed to obtain the highest possible neutron flux intensity with a subcriticality of 0.98. Low enrichment uranium is used for the fuel material because it enhances the neutron source performance. Safety, reliability, and environmental considerations are included in the facility conceptual design. Horizontal neutron channels are incorporated for performing basic research including cold neutron source. This paper describes the conceptual design and summarizes some of the related analyses.

  2. Temperature of optimum generation in facilities of solar cold making use of the equation characteristic; Temperatura de generacion optima en instalaciones de frio solar haciendo uso de la ecuacion caracteristica

    Energy Technology Data Exchange (ETDEWEB)

    Leucona, A.; Ventas, R.; Venegas, M. del C.; Zacarias, A.; Salgado, R.

    2008-07-01

    Absorption machines show a similar response as a function of the external temperatures, which can be approximated by a single functional dependence of the named characteristic temperature. This equation and the normalization curve of the solar thermal collector field driving the chiller and some more approximations allow determining the driving temperature that maximizes the cooling power at any time. This results in a simple equation. It is offered for the design of solar cooling facilities and their control algorithms. Some results are offered and discussed. (Author)

  3. Criteria for selection of target materials and design of high-efficiency-release targets for radioactive ion beam generation

    CERN Document Server

    Alton, G D; Liu, Y

    1999-01-01

    In this report, we define criteria for choosing target materials and for designing, mechanically stable, short-diffusion-length, highly permeable targets for generation of high-intensity radioactive ion beams (RIBs) for use at nuclear physics and astrophysics research facilities based on the ISOL principle. In addition, lists of refractory target materials are provided and examples are given of a number of successful targets, based on these criteria, that have been fabricated and tested for use at the Holifield Radioactive Ion Beam Facility (HRIBF).

  4. The Radiological Research Accelerator Facility

    Energy Technology Data Exchange (ETDEWEB)

    Hall, E.J.

    1992-05-01

    The Radiological Research Accelerator Facility (RARAF) is based on a 4-MV Van de Graaff accelerator, which is used to generate a variety of well-characterized radiation beams for research in radiobiology, radiological physics, and radiation chemistry. It is part of the Center for Radiological Research (CRR) -- formerly the Radiological Research Laboratory (RRL) -- of Columbia University, and its operation is supported as a National Facility by the US Department of Energy (DOE). As such, RARAF is available to all potential users on an equal basis, and scientists outside the CRR are encouraged to submit proposals for experiments at RARAF. The operation of the Van de Graaff is supported by the DOE, but the research projects themselves must be supported separately. Experiments performed from May 1991--April 1992 are described.

  5. The Radiological Research Accelerator Facility

    Energy Technology Data Exchange (ETDEWEB)

    Hall, E.J.; Marino, S.A.

    1993-05-01

    The Radiological Research Accelerator Facility (RARAF) is based on a 4-MV Van de Graaff accelerator, which is used to generate a variety of well-characterized radiation beams for research in radiobiology, radiological physics, and radiation chemistry. It is part of the Center for Radiological Research (CRR) - formerly the Radiological Research Laboratory of Columbia University, and its operation is supported as a National Facility by the US Department of Energy (DOE). As such, RARAF is available to all potential users on an equal basis and scientists outside the CRR are encouraged to submit proposals for experiments at RARAF. The operation of the Van de Graaff is supported by the DOE, but the research projects themselves must be supported separately. This report provides a listing and brief description of experiments performed at RARAF during the May 1, 1992 through April 30, 1993.

  6. Earthquake engineering for nuclear facilities

    CERN Document Server

    Kuno, Michiya

    2017-01-01

    This book is a comprehensive compilation of earthquake- and tsunami-related technologies and knowledge for the design and construction of nuclear facilities. As such, it covers a wide range of fields including civil engineering, architecture, geotechnical engineering, mechanical engineering, and nuclear engineering, for the development of new technologies providing greater resistance against earthquakes and tsunamis. It is crucial both for students of nuclear energy courses and for young engineers in nuclear power generation industries to understand the basics and principles of earthquake- and tsunami-resistant design of nuclear facilities. In Part I, "Seismic Design of Nuclear Power Plants", the design of nuclear power plants to withstand earthquakes and tsunamis is explained, focusing on buildings, equipment's, and civil engineering structures. In Part II, "Basics of Earthquake Engineering", fundamental knowledge of earthquakes and tsunamis as well as the dynamic response of structures and foundation ground...

  7. Power Systems Development Facility

    Energy Technology Data Exchange (ETDEWEB)

    Southern Company Services

    2004-04-30

    This report discusses Test Campaign TC15 of the Kellogg Brown & Root, Inc. (KBR) Transport Gasifier train with a Siemens Power Generation, Inc. (SPG) particle filter system at the Power Systems Development Facility (PSDF) located in Wilsonville, Alabama. The Transport Gasifier is an advanced circulating fluidized-bed reactor designed to operate as either a combustor or gasifier using a particulate control device (PCD). While operating as a gasifier, either air or oxygen can be used as the oxidant. Test run TC15 began on April 19, 2004, with the startup of the main air compressor and the lighting of the gasifier startup burner. The Transport Gasifier was shutdown on April 29, 2004, accumulating 200 hours of operation using Powder River Basin (PRB) subbituminous coal. About 91 hours of the test run occurred during oxygen-blown operations. Another 6 hours of the test run was in enriched-air mode. The remainder of the test run, approximately 103 hours, took place during air-blown operations. The highest operating temperature in the gasifier mixing zone mostly varied from 1,800 to 1,850 F. The gasifier exit pressure ran between 200 and 230 psig during air-blown operations and between 110 and 150 psig in oxygen-enhanced air operations.

  8. Facility design, construction, and operation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-04-01

    France has been disposing of low-level radioactive waste (LLW) at the Centre de Stockage de la Manche (CSM) since 1969 and now at the Centre de Stockage de l`Aube (CSA) since 1992. In France, several agencies and companies are involved in the development and implementation of LLW technology. The Commissariat a l`Energie Atomic (CEA), is responsible for research and development of new technologies. The Agence National pour la Gestion des Dechets Radioactifs is the agency responsible for the construction and operation of disposal facilities and for wastes acceptance for these facilities. Compagnie Generale des Matieres Nucleaires provides fuel services, including uranium enrichment, fuel fabrication, and fuel reprocessing, and is thus one generator of LLW. Societe pour les Techniques Nouvelles is an engineering company responsible for commercializing CEA waste management technology and for engineering and design support for the facilities. Numatec, Inc. is a US company representing these French companies and agencies in the US. In Task 1.1 of Numatec`s contract with Martin Marietta Energy Systems, Numatec provides details on the design, construction and operation of the LLW disposal facilities at CSM and CSA. Lessons learned from operation of CSM and incorporated into the design, construction and operating procedures at CSA are identified and discussed. The process used by the French for identification, selection, and evaluation of disposal technologies is provided. Specifically, the decisionmaking process resulting in the change in disposal facility design for the CSA versus the CSM is discussed. This report provides` all of the basic information in these areas and reflects actual experience to date.

  9. Facility Effluent Monitoring Plan determinations for the 600 Area facilities

    Energy Technology Data Exchange (ETDEWEB)

    Nickels, J.M.

    1991-08-01

    This document determines the need for Facility Effluent Monitoring Plans for Westinghouse Hanford Company's 600 Area facilities on the Hanford Site. The Facility Effluent Monitoring Plan determinations were prepared in accordance with A Guide For Preparing Hanford Site Facility Effluent Monitoring Plans (WHC 1991). Five major Westinghouse Hanford Company facilities in the 600 Area were evaluated: the Purge Water Storage Facility, 212-N, -P, and -R Facilities, the 616 Facility, and the 213-J K Storage Vaults. Of the five major facilities evaluated in the 600 Area, none will require preparation of a Facility Effluent Monitoring Plan.

  10. Synchrotron radiation facilities

    CERN Multimedia

    1972-01-01

    Particularly in the past few years, interest in using the synchrotron radiation emanating from high energy, circular electron machines has grown considerably. In our February issue we included an article on the synchrotron radiation facility at Frascati. This month we are spreading the net wider — saying something about the properties of the radiation, listing the centres where synchrotron radiation facilities exist, adding a brief description of three of them and mentioning areas of physics in which the facilities are used.

  11. Thermal distortion test facility

    Science.gov (United States)

    Stapp, James L.

    1995-02-01

    The thermal distortion test facility (TDTF) at Phillips Laboratory provides precise measurements of the distortion of mirrors that occurs when their surfaces are heated. The TDTF has been used for several years to evaluate mirrors being developed for high-power lasers. The facility has recently undergone some significant upgrades to improve the accuracy with which mirrors can be heated and the resulting distortion measured. The facility and its associated instrumentation are discussed.

  12. Waste Isolation Pilot Plant Annual Site Enviromental Report for 2008

    Energy Technology Data Exchange (ETDEWEB)

    Washington Regulatory and Enviromnetal Services

    2009-09-21

    The purpose of the Waste Isolation Pilot Plant Annual Site Environmental Report for 2008 (ASER) is to provide information required by U.S. Department of Energy (DOE) Order 231.1A, Environment, Safety, and Health Reporting. Specifically, the ASER presents summary environmental data to characterize site environmental management performance; summarize environmental occurrences and responses reported during the calendar year; confirm compliance with environmental standards and requirements; highlight significant facility programs and efforts; and describe how compliance and environmental improvement is accomplished through the WIPP Environmental Management System (EMS). The DOE Carlsbad Field Office (CBFO) and the management and operating contractor (MOC), Washington TRU Solutions LLC (WTS), maintain and preserve the environmental resources at the Waste Isolation Pilot Plant (WIPP). DOE Order 231.1A; DOE Order 450.1A, Environmental Protection Program; and DOE Order 5400.5, Radiation Protection of the Public and the Environment, require that the affected environment at and near DOE facilities be monitored to ensure the safety and health of the public and workers, and preservation of the environment. This report was prepared in accordance with DOE Order 231.1A, which requires that DOE facilities submit an ASER to the DOE Headquarters Chief Health, Safety, and Security Officer. The WIPP Hazardous Waste Facility Permit (HWFP) Number NM4890139088-TSDF (treatment, storage, and disposal facility) further requires that the ASER be provided to the New Mexico Environment Department (NMED). The WIPP mission is to safely dispose of transuranic (TRU) radioactive waste generated by the production of nuclear weapons and other activities related to the national defense of the United States. In 2008, 5,265 cubic meters (m3) of TRU waste were disposed of at the WIPP facility, including 5,216 m3 of contact-handled (CH) TRU waste and 49 m3 of remote-handled (RH) TRU waste. From the first

  13. Materials Characterization Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Materials Characterization Facility enables detailed measurements of the properties of ceramics, polymers, glasses, and composites. It features instrumentation...

  14. Mobile Solar Tracker Facility

    Data.gov (United States)

    Federal Laboratory Consortium — NIST's mobile solar tracking facility is used to characterize the electrical performance of photovoltaic panels. It incorporates meteorological instruments, a solar...

  15. Universal Drive Train Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This vehicle drive train research facility is capable of evaluating helicopter and ground vehicle power transmission technologies in a system level environment. The...

  16. Composite Structures Manufacturing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Composite Structures Manufacturing Facility specializes in the design, analysis, fabrication and testing of advanced composite structures and materials for both...

  17. Neutron Therapy Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Neutron Therapy Facility provides a moderate intensity, broad energy spectrum neutron beam that can be used for short term irradiations for radiobiology (cells)...

  18. Catalytic Fuel Conversion Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This facility enables unique catalysis research related to power and energy applications using military jet fuels and alternative fuels. It is equipped with research...

  19. Heated Tube Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Heated Tube Facility at NASA GRC investigates cooling issues by simulating conditions characteristic of rocket engine thrust chambers and high speed airbreathing...

  20. Engine Test Facility (ETF)

    Data.gov (United States)

    Federal Laboratory Consortium — The Air Force Arnold Engineering Development Center's Engine Test Facility (ETF) test cells are used for development and evaluation testing of propulsion systems for...

  1. High Combustion Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — At NETL's High-Pressure Combustion Research Facility in Morgantown, WV, researchers can investigate new high-pressure, high-temperature hydrogen turbine combustion...

  2. Region 9 NPDES Facilities

    Data.gov (United States)

    U.S. Environmental Protection Agency — Point geospatial dataset representing locations of NPDES Facilities. NPDES (National Pollution Discharge Elimination System) is an EPA permit program that regulates...

  3. Geospatial Data Analysis Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Geospatial application development, location-based services, spatial modeling, and spatial analysis are examples of the many research applications that this facility...

  4. Geodynamics Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — This GSL facility has evolved over the last three decades to support survivability and protective structures research. Experimental devices include three gas-driven...

  5. Imagery Data Base Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Imagery Data Base Facility supports AFRL and other government organizations by providing imagery interpretation and analysis to users for data selection, imagery...

  6. Pavement Testing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Comprehensive Environmental and Structural Analyses The ERDC Pavement Testing Facility, located on the ERDC Vicksburg campus, was originally constructed to provide...

  7. Nonlinear Materials Characterization Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Nonlinear Materials Characterization Facility conducts photophysical research and development of nonlinear materials operating in the visible spectrum to protect...

  8. Geophysical Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Geophysical Research Facility (GRF) is a 60 ft long qaodmasdkwaspemas5ajkqlsmdqpakldnzsdfls 22 ft wide qaodmasdkwaspemas4ajkqlsmdqpakldnzsdfls 7 ft deep concrete...

  9. Transonic Experimental Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Transonic Experimental Research Facility evaluates aerodynamics and fluid dynamics of projectiles, smart munitions systems, and sub-munitions dispensing systems;...

  10. Flexible Electronics Research Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Flexible Electronics Research Facility designs, synthesizes, tests, and fabricates materials and devices compatible with flexible substrates for Army information...

  11. DUPIC facility engineering

    Energy Technology Data Exchange (ETDEWEB)

    Park, J. J.; Lee, H. H.; Kim, K. H. and others

    2000-03-01

    The objectives of this study are (1) the refurbishment for PIEF(Post Irradiation Examination Facility) and M6 hot-cell in IMEF(Irradiated Material Examination Facility), (2) the establishment of the compatible facility for DUPIC fuel fabrication experiments which is licensed by government organization, and (3) the establishment of the transportation system and transportation cask for nuclear material between facilities. The report for this project describes following contents, such as objectives, necessities, scope, contents, results of current step, R and D plan in future and etc.

  12. Textiles Performance Testing Facilities

    Data.gov (United States)

    Federal Laboratory Consortium — The Textiles Performance Testing Facilities has the capabilities to perform all physical wet and dry performance testing, and visual and instrumental color analysis...

  13. Joint Computing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Raised Floor Computer Space for High Performance Computing The ERDC Information Technology Laboratory (ITL) provides a robust system of IT facilities to develop and...

  14. GPS Test Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Global Positioning System (GPS) Test Facility Instrumentation Suite (GPSIS) provides great flexibility in testing receivers by providing operational control of...

  15. Target Assembly Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Target Assembly Facility integrates new armor concepts into actual armored vehicles. Featuring the capability ofmachining and cutting radioactive materials, it...

  16. Facility Environmental Management System

    Data.gov (United States)

    Federal Laboratory Consortium — This is the Web site of the Federal Highway Administration's (FHWA's) Turner-Fairbank Highway Research Center (TFHRC) facility Environmental Management System (EMS)....

  17. Proximal Probes Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Proximal Probes Facility consists of laboratories for microscopy, spectroscopy, and probing of nanostructured materials and their functional properties. At the...

  18. Manufacturing Demonstration Facility (MDF)

    Data.gov (United States)

    Federal Laboratory Consortium — The U.S. Department of Energy Manufacturing Demonstration Facility (MDF) at Oak Ridge National Laboratory (ORNL) provides a collaborative, shared infrastructure to...

  19. Pavement Testing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — Comprehensive Environmental and Structural AnalysesThe ERDC Pavement Testing Facility, located on the ERDC Vicksburg campus, was originally constructed to provide an...

  20. Reversing nuclear opposition: evolving public acceptance of a permanent nuclear waste disposal facility.

    Science.gov (United States)

    Jenkins-Smith, Hank C; Silva, Carol L; Nowlin, Matthew C; deLozier, Grant

    2011-04-01

    Nuclear facilities have long been seen as the top of the list of locally unwanted land uses (LULUs), with nuclear waste repositories generating the greatest opposition. Focusing on the case of the Waste Isolation Pilot Plant (WIPP) in southern New Mexico, we test competing hypotheses concerning the sources of opposition and support for siting the facility, including demographics, proximity, political ideology, and partisanship, and the unfolding policy process over time. This study tracks the changes of risk perception and acceptance of WIPP over a decade, using measures taken from 35 statewide surveys of New Mexico citizens spanning an 11-year period from fall 1990 to summer 2001. This time span includes periods before and after WIPP became operational. We find that acceptance of WIPP is greater among those whose residences are closest to the WIPP facility. Surprisingly, and contrary to expectations drawn from the broader literature, acceptance is also greater among those who live closest to the nuclear waste transportation route. We also find that ideology, partisanship, government approval, and broader environmental concerns influence support for WIPP acceptance. Finally, the sequence of procedural steps taken toward formal approval of WIPP by government agencies proved to be important to gaining public acceptance, the most significant being the opening of the WIPP facility itself.