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Sample records for gene hot spot

  1. Are 'hot spots' hot spots?

    Science.gov (United States)

    Foulger, Gillian R.

    2012-07-01

    The term 'hot spot' emerged in the 1960s from speculations that Hawaii might have its origins in an unusually hot source region in the mantle. It subsequently became widely used to refer to volcanic regions considered to be anomalous in the then-new plate tectonic paradigm. It carried with it the implication that volcanism (a) is emplaced by a single, spatially restricted, mongenetic melt-delivery system, assumed to be a mantle plume, and (b) that the source is unusually hot. This model has tended to be assumed a priori to be correct. Nevertheless, there are many geological ways of testing it, and a great deal of work has recently been done to do so. Two fundamental problems challenge this work. First is the difficulty of deciding a 'normal' mantle temperature against which to compare estimates. This is usually taken to be the source temperature of mid-ocean ridge basalts (MORBs). However, Earth's surface conduction layer is ˜200 km thick, and such a norm is not appropriate if the lavas under investigation formed deeper than the 40-50 km source depth of MORB. Second, methods for estimating temperature suffer from ambiguity of interpretation with composition and partial melt, controversy regarding how they should be applied, lack of repeatability between studies using the same data, and insufficient precision to detect the 200-300 °C temperature variations postulated. Available methods include multiple seismological and petrological approaches, modelling bathymetry and topography, and measuring heat flow. Investigations have been carried out in many areas postulated to represent either (hot) plume heads or (hotter) tails. These include sections of the mid-ocean spreading ridge postulated to include ridge-centred plumes, the North Atlantic Igneous Province, Iceland, Hawaii, oceanic plateaus, and high-standing continental areas such as the Hoggar swell. Most volcanic regions that may reasonably be considered anomalous in the simple plate-tectonic paradigm have been

  2. Coulomb explosion of "hot spot"

    CERN Document Server

    Oreshkin, V I; Chaikovsky, S A; Artyomov, A P

    2016-01-01

    The study presented in this paper has shown that the generation of hard x rays and high-energy ions, which are detected in pinch implosion experiments, may be associated with the Coulomb explosion of the hot spot that is formed due to the outflow of the material from the pinch cross point. During the process of material outflow, the temperature of the hot spot plasma increases, and conditions arise for the plasma electrons to become continuously accelerated. The runaway of electrons from the hot spot region results in the buildup of positive space charge in this region followed by a Coulomb explosion. The conditions for the hot spot plasma electrons to become continuously accelerated have been revealed and estimates have been obtained for the kinetic energy of the ions generated by the Coulomb explosion.

  3. Mutational hot spot in the DSPP gene causing dentinogenesis imperfecta type II.

    Science.gov (United States)

    Kim, Jung-Wook; Hu, Jan C-C; Lee, Jae-Il; Moon, Sung-Kwon; Kim, Young-Jae; Jang, Ki-Taeg; Lee, Sang-Hoon; Kim, Chong-Chul; Hahn, Se-Hyun; Simmer, James P

    2005-02-01

    The current system for the classification of hereditary defects of tooth dentin is based upon clinical and radiographic findings and consists of two types of dentin dysplasia (DD) and three types of dentinogenesis imperfecta (DGI). However, whether DGI type III should be considered a distinct phenotype or a variation of DGI type II is debatable. In the 30 years since the classification system was first proposed, significant advances have been made regarding the genetic etiologies of inherited dentin defects. DGI type II is recognized as an autosomal dominant disorder with almost complete penetrance and a low frequency of de novo mutations. We have identified a mutation (c.52G-->T, p.V18F) at the first nucleotide of exon 3 of the DSPP (dentin sialophosphoprotein) gene in a Korean family (de novo) and a Caucasian family. This mutation has previously been reported as causing DGI type II in a Chinese family. These findings suggest that this mutation site represents a mutational "hot spot" in the DSPP gene. The clinical and radiographic features of these two families include the classic phenotypes associated with both DGI type II and type III. Finding that a single mutation causes both phenotypic patterns strongly supports the conclusion that DGI type II and DGI type III are not separate diseases but rather the phenotypic variation of a single disease. We propose a modification of the current classification system such that the designation "hereditary opalescent dentin" or "DGI type II" should be used to describe both the DGI type II and type III phenotypes.

  4. The structure of HIV-1 genomic RNA in the gp120 gene determines a recombination hot spot in vivo.

    Science.gov (United States)

    Galetto, Román; Moumen, Abdeladim; Giacomoni, Véronique; Véron, Michel; Charneau, Pierre; Negroni, Matteo

    2004-08-27

    By frequently rearranging large regions of the genome, genetic recombination is a major determinant in the plasticity of the human immunodeficiency virus type I (HIV-1) population. In retroviruses, recombination mostly occurs by template switching during reverse transcription. The generation of retroviral vectors provides a means to study this process after a single cycle of infection of cells in culture. Using HIV-1-derived vectors, we present here the first characterization and estimate of the strength of a recombination hot spot in HIV-1 in vivo. In the hot spot region, located within the C2 portion of the gp120 envelope gene, the rate of recombination is up to ten times higher than in the surrounding regions. The hot region corresponds to a previously identified RNA hairpin structure. Although recombination breakpoints in vivo cluster in the top portion of the hairpin, the bias for template switching in this same region appears less marked in a cell-free system. By modulating the stability of this hairpin we were able to affect the local recombination rate both in vitro and in infected cells, indicating that the local folding of the genomic RNA is a major parameter in the recombination process. This characterization of reverse transcription products generated after a single cycle of infection provides insights in the understanding of the mechanism of recombination in vivo and suggests that specific regions of the genome might be prompted to yield different rates of evolution due to the presence of circumscribed recombination hot spots.

  5. The puzzling MILAGRO hot spots

    CERN Document Server

    Drury, Luke

    2008-01-01

    We discuss the reported detection by the MILAGRO experiment of localised hot spots in the cosmic ray arrival distribution and the difficulty of interpreting these observations. A model based on secondary neutron production in the heliotail is shown to fail. An alternative model based on loss-cone leakage through a magnetic trap from a local source region is proposed.

  6. ESA uncovers Geminga's `hot spot'

    Science.gov (United States)

    2004-07-01

    16 July 2004 Astronomers using ESA’s X-ray observatory XMM-Newton have detected a small, bright ‘hot spot’ on the surface of the neutron star called Geminga, 500 light-years away. The hot spot is the size of a football field and is caused by the same mechanism producing Geminga’s X-ray tails. This discovery identifies the missing link between the X-ray and gamma-ray emission from Geminga. hi-res Size hi-res: 1284 kb Credits: ESA, P. Caraveo (IASF, Milan) Geminga's hot spot This figure shows the effects of charged particles accelerated in the magnetosphere of Geminga. Panel (a) shows an image taken with the EPIC instrument on board the XMM-Newton observatory. The bright tails, made of particles kicked out by Geminga’s strong magnetic field, trail the neutron star as it moves about in space. Panel (b) shows how electrically charged particles interact with Geminga’s magnetic field. For example, if electrons (blue) are kicked out by the star, positrons (in red) hit the star’s magnetic poles like in an ‘own goal’. Panel (c) illustrates the size of Geminga’s magnetic field (blue) compared to that of the star itself at the centre (purple). The magnetic field is tilted with respect to Geminga’s rotation axis (red). Panel (d) shows the magnetic poles of Geminga, where charged particles hit the surface of the star, creating a two-million degrees hot spot, a region much hotter than the surroundings. As the star spins on its rotation axis, the hot spot comes into view and then disappears, causing the periodic colour change seen by XMM-Newton. An animated version of the entire sequence can be found at: Click here for animated GIF [low resolution, animated GIF, 5536 KB] Click here for AVI [high resolution, AVI with DIVX compression, 19128 KB] hi-res Size hi-res: 371 kb Credits: ESA, P. Caraveo (IASF, Milan) Geminga's hot spot, panel (a) Panel (a) shows an image taken with the EPIC instrument on board the XMM-Newton observatory. The bright tails, made of

  7. A Hot Spot in Coma

    CERN Document Server

    Donnelly, R H; Forman, W R; Jones, C; Churazov, E; Gilfanov, M R

    1999-01-01

    We study the temperature structure of the central part (r<18' ~0.7 h50**-1 Mpc) of the Coma cluster of galaxies using ASCA data. Two different analysis methods produce results in good agreement with each other and reveal the presence of interesting structures in the gas temperature distribution. Globally, the average temperature in the center of the cluster is 9.0 +/- 0.6 keV in good agreement with previous results. Superimposed on this, we find a cool area with temperatures of 4-6 keV associated with a filament of X-ray emission extending southeast from the cluster center detected by Vikhlinin and coworkers. We also find a hot spot with a temperature of around 13 keV displaced north from the central peak of emission. The distribution of the gas temperatures and relative specific entropies suggests that the cool features are most likely gas stripped from a galaxy group centered on NGC 4874 falling toward the core from outside, while the hot spot located ``ahead'' of this in-falling gas is due to shock heat...

  8. Hot Spot Removal System: System description

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-09-01

    Hazardous wastes contaminated with radionuclides, chemicals, and explosives exist across the Department of Energy complex and need to be remediated due to environmental concerns. Currently, an opportunity is being developed to dramatically reduce remediation costs and to assist in the acceleration of schedules associated with these wastes by deploying a Hot Spot Removal System. Removing the hot spot from the waste site will remove risk driver(s) and enable another, more cost effective process/option/remedial alternative (i.e., capping) to be applied to the remainder of the site. The Hot Spot Removal System consists of a suite of technologies that will be utilized to locate and remove source terms. Components of the system can also be used in a variety of other cleanup activities. This Hot Spot Removal System Description document presents technologies that were considered for possible inclusion in the Hot Spot Removal System, technologies made available to the Hot Spot Removal System, industrial interest in the Hot Spot Removal System`s subsystems, the schedule required for the Hot Spot Removal System, the evaluation of the relevant technologies, and the recommendations for equipment and technologies as stated in the Plan section.

  9. HotSpot Software Test Plan

    Energy Technology Data Exchange (ETDEWEB)

    Walker, H; Homann, S G

    2009-03-12

    This Software Test Plan (STP) describes the procedures used to verify and validate that the HotSpot Health Physics Codes meet the requirements of its user base, which includes: (1) Users of the PC version of HotSpot conducting consequence assessment, hazard assessment and safety analysis calculations; and (2) Users of the NARAC Web and iClient software tools, which allow users to run HotSpot for consequence assessment modeling. This plan is intended to meet Critical Recommendation 2 from the Software Evaluation of HotSpot and DOE Safety Software Toolbox Recommendation for inclusion of HotSpot in the Department of Energy (DOE) Safety Software Toolbox. These users and sponsors of the HotSpot software and the organizations they represent constitute the intended audience for this document. HotSpot software is maintained for the Department of Energy Office of Emergency Operations by the National Atmospheric Release Advisory Center (NARAC) at Lawrence Livermore National Laboratory (LLNL). An overview of HotSpot and NARAC are provided.

  10. HotSpot Software Configuration Management Plan

    Energy Technology Data Exchange (ETDEWEB)

    Walker, H; Homann, S G

    2009-03-12

    This Software Configuration Management Plan (SCMP) describes the software configuration management procedures used to ensure that the HotSpot dispersion model meets the requirements of its user base, which includes: (1) Users of the PC version of HotSpot for consequence assessment, hazard assessment and safety analysis calculations; and (2) Users of the NARAC Web and iClient software tools, which allow users to run HotSpot for consequence assessment modeling These users and sponsors of the HotSpot software and the organizations they represent constitute the intended audience for this document. This plan is intended to meet Critical Recommendations 1 and 3 from the Software Evaluation of HotSpot and DOE Safety Software Toolbox Recommendation for inclusion of HotSpot in the Department of Energy (DOE) Safety Software Toolbox. HotSpot software is maintained for the Department of Energy Office of Emergency Operations by the National Atmospheric Release Advisory Center (NARAC) at Lawrence Livermore National Laboratory (LLNL). An overview of HotSpot and NARAC are provided.

  11. A cancer-predisposing "hot spot" mutation of the fumarase gene creates a dominant negative protein.

    Science.gov (United States)

    Lorenzato, Annalisa; Olivero, Martina; Perro, Mario; Brière, Jean Jacques; Rustin, Pierre; Di Renzo, Maria Flavia

    2008-02-15

    The Fumarase (Fumarate Hydratase, FH) is a tumor suppressor gene whose germline heterozygous mutations predispose to hereditary leiomyomatosis and renal cell cancer (HLRCC). The FH gene encodes an enzyme of the Krebs cycle, functioning as a homotetramer and catalyzing the hydration of fumarate to malate. Among the numerous FH mutations reported so far, the R190H missense mutation is the most frequent in HLRCC patients. Here we show the functional analyses of the R190H, in comparison to the better characterized E319Q mutation. We first expressed wild-type and mutated proteins in FH deficient human skin fibroblasts, using lentiviral vectors. The wild-type transgene was able to restore the FH enzymatic activity in cells, while the R190H- and E319Q-FH were not. More interestingly, when the same transgenes were expressed in normal, FH-proficient cells, only the R190H-FH reduced the endogenous FH enzymatic activity. By enforcing the expression of equal amount of wild-type and R190H-FH in the same cell, we showed that the mutated FH protein directly inhibited enzymatic activity by nearly abrogating the FH homotetramer formation. These data demonstrate the dominant negative effect of the R190H missense mutation in the FH gene and suggest that the FH tumor-suppressing activity might be impaired in cells carrying a heterozygous mutation. (c) 2007 Wiley-Liss, Inc.

  12. Modeling deflagration waves out of hot spots

    Science.gov (United States)

    Partom, Yehuda

    2017-01-01

    It is widely accepted that shock initiation and detonation of heterogeneous explosives comes about by a two-step process known as ignition and growth. In the first step a shock sweeping through an explosive cell (control volume) creates hot spots that become ignition sites. In the second step, deflagration waves (or burn waves) propagate out of those hot spots and transform the reactant in the cell into reaction products. The macroscopic (or average) reaction rate of the reactant in the cell depends on the speed of those deflagration waves and on the average distance between neighboring hot spots. Here we simulate the propagation of deflagration waves out of hot spots on the mesoscale in axial symmetry using a 2D hydrocode, to which we add heat conduction and bulk reaction. The propagation speed of the deflagration waves may depend on both pressure and temperature. It depends on pressure for quasistatic loading near ambient temperature, and on temperature at high temperatures resulting from shock loading. From the simulation we obtain deflagration fronts emanating out of the hot spots. For 8 to 13 GPa shocks, the emanating fronts propagate as deflagration waves to consume the explosive between hot spots. For higher shock levels deflagration waves may interact with the sweeping shock to become detonation waves on the mesoscale. From the simulation results we extract average deflagration wave speeds.

  13. Discovery of feature-based hot spots using supervised clustering

    Science.gov (United States)

    Ding, Wei; Stepinski, Tomasz F.; Parmar, Rachana; Jiang, Dan; Eick, Christoph F.

    2009-07-01

    Feature-based hot spots are localized regions where the attributes of objects attain high values. There is considerable interest in automatic identification of feature-based hot spots. This paper approaches the problem of finding feature-based hot spots from a data mining perspective, and describes a method that relies on supervised clustering to produce a list of hot spot regions. Supervised clustering uses a fitness function rewarding isolation of the hot spots to optimally subdivide the dataset. The clusters in the optimal division are ranked using the interestingness of clusters that encapsulate their utility for being hot spots. Hot spots are associated with the top ranked clusters. The effectiveness of supervised clustering as a hot spot identification method is evaluated for four conceptually different clustering algorithms using a dataset describing the spatial distribution of ground ice on Mars. Clustering solutions are visualized by specially developed raster approximations. Further assessment of the ability of different algorithms to yield hot spots is performed using raster approximations. Density-based clustering algorithm is found to be the most effective for hot spot identification. The results of the hot spot discovery by supervised clustering are comparable to those obtained using the G* statistic, but the new method offers a high degree of automation, making it an ideal tool for mining large datasets for the existence of potential hot spots.

  14. Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription.

    Science.gov (United States)

    Xie, Qiu; Li, Caihua; Song, Xiaozhen; Wu, Lihua; Jiang, Qian; Qiu, Zhiyong; Cao, Haiyan; Yu, Kaihui; Wan, Chunlei; Li, Jianting; Yang, Feng; Huang, Zebing; Niu, Bo; Jiang, Zhengwen; Zhang, Ting

    2016-12-06

    The biogenesis of ribosomes in vivo is an essential process for cellular functions. Transcription of ribosomal RNA (rRNA) genes is the rate-limiting step in ribosome biogenesis controlled by environmental conditions. Here, we investigated the role of folate antagonist on changes of DNA double-strand breaks (DSBs) landscape in mouse embryonic stem cells. A significant DSB enhancement was detected in the genome of these cells and a large majority of these DSBs were found in rRNA genes. Furthermore, spontaneous DSBs in cells under folate deficiency conditions were located exclusively within the rRNA gene units, representing a H3K4me1 hallmark. Enrichment H3K4me1 at the hot spots of DSB regions enhanced the recruitment of upstream binding factor (UBF) to rRNA genes, resulting in the increment of rRNA genes transcription. Supplement of folate resulted in a restored UBF binding across DNA breakage sites of rRNA genes, and normal rRNA gene transcription. In samples from neural tube defects (NTDs) with low folate level, up-regulation of rRNA gene transcription was observed, along with aberrant UBF level. Our results present a new view by which alterations in folate levels affects DNA breakage through epigenetic control leading to the regulation of rRNA gene transcription during the early stage of development.

  15. Research Hot Spots of Black Peanut

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Taking the research papers on black peanut in National Knowledge Infrastructure(CNKI)as data sources,we determine the research hot spots on black peanut using word frequency analysis,and analyze the main research directions that change over the years.The results show that the relevant researches on black peanut are mainly concentrated in six themes(study of varieties,physiological property,cultivation management,development prospects,relationship with agriculture,and molecular level);varieties,physiology,cultivation and other aspects are the focus of current research hot spots;the researches on selection and breeding of variety and various physiological and ecological mechanisms,still need a breakthrough.

  16. A duchenne muscular dystrophy gene hot spot mutation in dystrophin-deficient cavalier king charles spaniels is amenable to exon 51 skipping.

    Directory of Open Access Journals (Sweden)

    Gemma L Walmsley

    Full Text Available BACKGROUND: Duchenne muscular dystrophy (DMD, which afflicts 1 in 3500 boys, is one of the most common genetic disorders of children. This fatal degenerative condition is caused by an absence or deficiency of dystrophin in striated muscle. Most affected patients have inherited or spontaneous deletions in the dystrophin gene that disrupt the reading frame resulting in unstable truncated products. For these patients, restoration of the reading frame via antisense oligonucleotide-mediated exon skipping is a promising therapeutic approach. The major DMD deletion "hot spot" is found between exons 45 and 53, and skipping exon 51 in particular is predicted to ameliorate the dystrophic phenotype in the greatest number of patients. Currently the mdx mouse is the most widely used animal model of DMD, although its mild phenotype limits its suitability in clinical trials. The Golden Retriever muscular dystrophy (GRMD model has a severe phenotype, but due to its large size, is expensive to use. Both these models have mutations in regions of the dystrophin gene distant from the commonly mutated DMD "hot spot". METHODOLOGY/PRINCIPAL FINDINGS: Here we describe the severe phenotype, histopathological findings, and molecular analysis of Cavalier King Charles Spaniels with dystrophin-deficient muscular dystrophy (CKCS-MD. The dogs harbour a missense mutation in the 5' donor splice site of exon 50 that results in deletion of exon 50 in mRNA transcripts and a predicted premature truncation of the translated protein. Antisense oligonucleotide-mediated skipping of exon 51 in cultured myoblasts from an affected dog restored the reading frame and protein expression. CONCLUSIONS/SIGNIFICANCE: Given the small size of the breed, the amiable temperament and the nature of the mutation, we propose that CKCS-MD is a valuable new model for clinical trials of antisense oligonucleotide-induced exon skipping and other therapeutic approaches for DMD.

  17. Meteorology of Jupiter's Equatorial Hot Spots and Plumes from Cassini

    CERN Document Server

    Choi, David S; Vasavada, Ashwin R; Simon-Miller, Amy A

    2013-01-01

    We present an updated analysis of Jupiter's equatorial meteorology from Cassini observations. For two months preceding the spacecraft's closest approach, the Imaging Science Subsystem (ISS) onboard regularly imaged the atmosphere. We created time-lapse movies from this period in order to analyze the dynamics of equatorial hot spots and their interactions with adjacent latitudes. Hot spots are quasi-stable, rectangular dark areas on visible-wavelength images, with defined eastern edges that sharply contrast with surrounding clouds, but diffuse western edges serving as nebulous boundaries with adjacent equatorial plumes. Hot spots exhibit significant variations in size and shape over timescales of days and weeks. Some of these changes correspond with passing vortex systems from adjacent latitudes interacting with hot spots. Strong anticyclonic gyres present to the south and southeast of the dark areas appear to circulate into hot spots. Impressive, bright white plumes occupy spaces in between hot spots. Compact...

  18. Methodology and software to detect viral integration site hot-spots

    Directory of Open Access Journals (Sweden)

    Kim Namshin

    2011-09-01

    Full Text Available Abstract Background Modern gene therapy methods have limited control over where a therapeutic viral vector inserts into the host genome. Vector integration can activate local gene expression, which can cause cancer if the vector inserts near an oncogene. Viral integration hot-spots or 'common insertion sites' (CIS are scrutinized to evaluate and predict patient safety. CIS are typically defined by a minimum density of insertions (such as 2-4 within a 30-100 kb region, which unfortunately depends on the total number of observed VIS. This is problematic for comparing hot-spot distributions across data sets and patients, where the VIS numbers may vary. Results We develop two new methods for defining hot-spots that are relatively independent of data set size. Both methods operate on distributions of VIS across consecutive 1 Mb 'bins' of the genome. The first method 'z-threshold' tallies the number of VIS per bin, converts these counts to z-scores, and applies a threshold to define high density bins. The second method 'BCP' applies a Bayesian change-point model to the z-scores to define hot-spots. The novel hot-spot methods are compared with a conventional CIS method using simulated data sets and data sets from five published human studies, including the X-linked ALD (adrenoleukodystrophy, CGD (chronic granulomatous disease and SCID-X1 (X-linked severe combined immunodeficiency trials. The BCP analysis of the human X-linked ALD data for two patients separately (774 and 1627 VIS and combined (2401 VIS resulted in 5-6 hot-spots covering 0.17-0.251% of the genome and containing 5.56-7.74% of the total VIS. In comparison, the CIS analysis resulted in 12-110 hot-spots covering 0.018-0.246% of the genome and containing 5.81-22.7% of the VIS, corresponding to a greater number of hot-spots as the data set size increased. Our hot-spot methods enable one to evaluate the extent of VIS clustering, and formally compare data sets in terms of hot-spot overlap

  19. A microsatellite platform for hot spot detection

    Energy Technology Data Exchange (ETDEWEB)

    Walter, I.; Briess, K.; Baerwald, W.; Lorenz, E.; Skrbek, W.; Schrandt, F. [DLR, Berlin (Germany). Inst. of Space Sensor Technology & Planetary Exploration

    2005-01-01

    The main payload of the BIRD micro-satellite is the newly developed hot spot recognition system. It's a dual-channel instrument for middle and thermal infrared imagery based on cooled MCT line detectors. The miniaturisation by integrated detector/cooler assemblies provides a highly efficient design. Since the launch in October 2001 from SHAR/India the BIRD payload, claiming 30% of the BIRD mass of 92 kg, is fully operational. Among others forest fires (Australia), volcanoes (Etna, Chile) and burning coal mines (China) have been detected and their parameters like size, temperature and energy release could be determined. As the status of the payload system is satisfactorily it has a potential to be applied in new missions with the help of modern detector technology.

  20. Magnetic spots on hot massive stars

    CERN Document Server

    Cantiello, Matteo

    2011-01-01

    Hot luminous stars show a variety of phenomena in their photospheres and winds which still lack clear physical explanation. Among these phenomena are photospheric turbulence, line profile variability (LPV), non-thermal emission, non-radial pulsations, discrete absorption components (DACs) and wind clumping. Cantiello et al. (2009) argued that a convection zone close to the stellar surface could be responsible for some of these phenomena. This convective zone is caused by a peak in the opacity associated with iron-group elements and is referred to as the "iron convection zone" (FeCZ). Assuming dynamo action producing magnetic fields at equipartition in the FeCZ, we investigate the occurrence of subsurface magnetism in OB stars. Then we study the surface emergence of these magnetic fields and discuss possible observational signatures of magnetic spots. Simple estimates are made using the subsurface properties of massive stars, as calculated in 1D stellar evolution models. We find that magnetic fields of suffici...

  1. Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon α.

    Science.gov (United States)

    Zhijian, Yu; Zhen, Huang; Fan, Zhang; Jin, Yang; Qiwen, Deng; Zhongming, Zeng

    2010-10-20

    It has been reported that hepatitis B virus (HBV) core protein (HBc) can inhibit the transcription of human interferon-induced MxA gene. In this study, we investigated whether HBc protein mutations at hot spots (L60V, S87G and I97L) could still inhibit MxA transcription and the potential significance of this inhibition in virus replication in vitro. Our data indicated that the IFN-induced MxA mRNA expression level and MxA promoter activity was significantly down-regulated by mutant protein of HBc(I97L), compared to WT and the other two mutated HBc proteins(L60V or S87G). However, in Huh7 cells stably expressing WT or the mutated HBc proteins (L60V, S87G or I97L), IFN-α could inhibit the extra- and intracellular HBV DNA level and HBsAg secretion to a similar level compared to that in cells transfected with control plasmids. In conclusion, HBc protein with I97L mutation may play an special role in suppressing the transcription of MxA gene. Moreover, the inhibitory effect on MxA gene transcription by the WT or mutated HBc proteins (L60V, S87G and I97L) has no impact on inhibition of HBV replication by IFN-α in Huh7 cells. The clinical significance of the inhibitory effect of MxA gene transcription by HBc protein requires further study.

  2. The crime prevention value of hot spots policing.

    Science.gov (United States)

    Braga, Anthony A

    2006-08-01

    This paper reviews the available research evidence on the effectiveness of hot spots policing programs in reducing crime and disorder. The research identified five randomized controlled experiments and four non-equivalent control group quasi-experiments evaluating the effects of hot spots policing interventions on crime. Seven of nine selected evaluations reported noteworthy crime and disorder reductions. Meta-analyses of the randomized experiments revealed statistically significant mean effect sizes favoring hot spots policing interventions in reducing citizen calls for service in treatment places relative to control places. When immediate spatial displacement was measured, it was very limited and unintended crime prevention benefits were associated with the hot spots policing programs. The results of this review suggest that hot spots policing is an effective crime prevention strategy.

  3. Complete mitochondrial genome sequence of three Tetrahymena species reveals mutation hot spots and accelerated nonsynonymous substitutions in Ymf genes.

    Directory of Open Access Journals (Sweden)

    Mike M Moradian

    Full Text Available The ciliate Tetrahymena, a model organism, contains divergent mitochondrial (Mt genome with unusual properties, where half of its 44 genes still remain without a definitive function. These genes could be categorized into two major groups of KPC (known protein coding and Ymf (genes without an identified function. To gain insights into the mechanisms underlying gene divergence and molecular evolution of Tetrahymena (T. Mt genomes, we sequenced three Mt genomes of T.paravorax, T.pigmentosa, and T.malaccensis. These genomes were aligned and the analyses were carried out using several programs that calculate distance, nucleotide substitution (dn/ds, and their rate ratios (omega on individual codon sites and via a sliding window approach. Comparative genomic analysis indicated a conserved putative transcription control sequence, a GC box, in a region where presumably transcription and replication initiate. We also found distinct features in Mt genome of T.paravorax despite similar genome organization among these approximately 47 kb long linear genomes. Another significant finding was the presence of at least one or more highly variable regions in Ymf genes where majority of substitutions were concentrated. These regions were mutation hotspots where elevated distances and the dn/ds ratios were primarily due to an increase in the number of nonsynonymous substitutions, suggesting relaxed selective constraint. However, in a few Ymf genes, accelerated rates of nonsynonymous substitutions may be due to positive selection. Similarly, on protein level the majority of amino acid replacements occurred in these regions. Ymf genes comprise half of the genes in Tetrahymena Mt genomes, so understanding why they have not been assigned definitive functions is an important aspect of molecular evolution. Importantly, nucleotide substitution types and rates suggest possible reasons for not being able to find homologues for Ymf genes. Additionally, comparative genomic

  4. An approach for addressing hard-to-detect hot spots.

    Science.gov (United States)

    Abelquist, Eric W; King, David A; Miller, Laurence F; Viars, James A

    2013-05-01

    The Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) survey approach is comprised of systematic random sampling coupled with radiation scanning to assess acceptability of potential hot spots. Hot spot identification for some radionuclides may not be possible due to the very weak gamma or x-ray radiation they emit-these hard-to-detect nuclides are unlikely to be identified by field scans. Similarly, scanning technology is not yet available for chemical contamination. For both hard-to-detect nuclides and chemical contamination, hot spots are only identified via volumetric sampling. The remedial investigation and cleanup of sites under the Comprehensive Environmental Response, Compensation, and Liability Act typically includes the collection of samples over relatively large exposure units, and concentration limits are applied assuming the contamination is more or less uniformly distributed. However, data collected from contaminated sites demonstrate contamination is often highly localized. These highly localized areas, or hot spots, will only be identified if sample densities are high or if the environmental characterization program happens to sample directly from the hot spot footprint. This paper describes a Bayesian approach for addressing hard-to-detect nuclides and chemical hot spots. The approach begins using available data (e.g., as collected using the standard approach) to predict the probability that an unacceptable hot spot is present somewhere in the exposure unit. This Bayesian approach may even be coupled with the graded sampling approach to optimize hot spot characterization. Once the investigator concludes that the presence of hot spots is likely, then the surveyor should use the data quality objectives process to generate an appropriate sample campaign that optimizes the identification of risk-relevant hot spots.

  5. Meteorology of Jupiter's Equatorial Hot Spots and Plumes from Cassini

    Science.gov (United States)

    Choi, David Sanghun; Showman, Adam P.; Vasavada, Ashwin R.; Simon-Miller, Amy A.

    2013-01-01

    We present an updated analysis of Jupiter's equatorial meteorology from Cassini observations. For two months preceding the spacecraft's closest approach, the Imaging Science Subsystem (ISS) onboard regularly imaged the atmosphere. We created time-lapse movies from this period in order to analyze the dynamics of equatorial hot spots and their interactions with adjacent latitudes. Hot spots are relatively cloud-free regions that emit strongly at 5 lm; improved knowledge of these features is crucial for fully understanding Galileo probe measurements taken during its descent through one. Hot spots are quasistable, rectangular dark areas on visible-wavelength images, with defined eastern edges that sharply contrast with surrounding clouds, but diffuse western edges serving as nebulous boundaries with adjacent equatorial plumes. Hot spots exhibit significant variations in size and shape over timescales of days and weeks. Some of these changes correspond with passing vortex systems from adjacent latitudes interacting with hot spots. Strong anticyclonic gyres present to the south and southeast of the dark areas appear to circulate into hot spots. Impressive, bright white plumes occupy spaces in between hot spots. Compact cirrus-like 'scooter' clouds flow rapidly through the plumes before disappearing within the dark areas. These clouds travel at 150-200 m/s, much faster than the 100 m/s hot spot and plume drift speed. This raises the possibility that the scooter clouds may be more illustrative of the actual jet stream speed at these latitudes. Most previously published zonal wind profiles represent the drift speed of the hot spots at their latitude from pattern matching of the entire longitudinal image strip. If a downward branch of an equatorially-trapped Rossby wave controls the overall appearance of hot spots, however, the westward phase velocity of the wave leads to underestimates of the true jet stream speed.

  6. Allele-dependent recombination frequency: homology requirement in meiotic recombination at the hot spot in the mouse major histocompatibility complex.

    Science.gov (United States)

    Yoshino, M; Sagai, T; Lindahl, K F; Toyoda, Y; Moriwaki, K; Shiroishi, T

    1995-05-20

    Meiotic recombination break joints in the mouse major histocompatibility complex (MHC) are clustered within short segments known as hot spots. We systematically investigated the requirement for sequence homology between two chromosomes for recombination activity at the hot spot next to the Lmp2 gene. The results indicated that a high rate of recombination required a high degree of similarity of overall genome structure at the hot spot. In particular, the same copy number of repetitive sequences within the hot spot was essential for a high frequency of recombination, suggesting that recombination in mouse meiosis is more sensitive to heterozygous deletion or insertion of DNA than to mismatches of single-base substitutions.

  7. On the burn topology of hot-spot-initiated reactions

    Energy Technology Data Exchange (ETDEWEB)

    Hill, Larry G [Los Alamos National Laboratory; Zimmermann, Bjorn [WOLFRAM RESEARCH INC.; Nichols, Albert L [LLNL

    2009-01-01

    We determine the reaction progress function for an ideal hot spot model problem. The considered problem has an exact analytic solution that can derived from a reduction of Nichols statistical hot spot model. We perform numerical calculations to verify the analytic solution and to illustrate the error realized in real, finite systems. We show how the baseline problem, which does not distinguish between the reactant and product densities, can be scaled to handle general cases for which the two densities differ.

  8. Models and methods for hot spot safety work

    OpenAIRE

    Vistisen, Dorte; Thyregod, Poul; LAURSEN, Jan Grubb

    2002-01-01

    Despite the fact that millions DKK each year are spent on improving roadsafety in Denmark, funds for traffic safety are limited. It is therefore vital to spend the resources as effectively as possible. This thesis is concerned with the area of traffic safety denoted "hot spot safety work", which is the task of improving road safety through alterations of the geometrical and environmental characteristics of the existing road network. The presently applied models and methods in hot spot safety ...

  9. SYSTEMATIC AND DYNAMIC PROPER-TIES OF CASTING HOT SPOT

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The variation of casting hot spot with proceeding of solidification and components of casting-mold system is studied by the technique of numerical simulation of solidification.The result shows that the thickest part of casting is not exactly the last part of solidification in the casting, while the last part of solidification is not exactly casting hot spot at the early stage of solidification.The location, size, shape and number of casting hot spot change with geomitric, physical and technological factors of the casting-mold system such as thickness of the casting secondary wall and with the passage of time in the course of the solidification.The former is known as the systematic property of hot spot and the latter, dynamic property.Only when the properties of hot spot are grasped completely and accurately, can it be fed more effectively.By doing so, not only sound castings can be obtained, but also riser efficiency can be improved.

  10. Cygnus A: A Long Wavelength Resolution of the Hot Spots

    CERN Document Server

    Lazio, T J W; Kassim, N E; Perley, R A; Erickson, W C; Carilli, C L; Crane, P C

    2006-01-01

    This paper presents observations of Cygnus A at 74 and 327 MHz at angular resolutions of approximately 10" and 3", respectively. These observations are among the highest angular resolutions obtained below 1000 MHz for this object. While the angular resolution at 74 MHz is not sufficient to separate clearly the hot spots from the lobes, guided by 151 and 327 MHz images, we have estimated the 74 MHz emission from the hot spots. We confirm that the emission from both the western and eastern hot spots flattens at low frequencies and that there is a spectral asymmetry between the two. For the eastern hot spot, a low-energy cutoff in the electron energy spectrum appears to explain the flattening, which implies a cutoff Lorentz factor \\gamma_min ~ 300, though we cannot exclude the possibility that there might be a moderate level of free-free absorption. For the western hot spot, the current observations are not sufficient to distinguish between a free-free absorped power-law spectrum and a synchrotron self-absorbed ...

  11. Hot spots of perforated forest in the eastern United States.

    Science.gov (United States)

    Riitters, Kurt H; Coulston, John W

    2005-04-01

    National assessments of forest fragmentation satisfy international biodiversity conventions, but they do not identify specific places where ecological impacts are likely. In this article, we identify geographic concentrations (hot spots) of forest located near holes in otherwise intact forest canopies (perforated forest) in the eastern United States, and we describe the proximate causes in terms of the non-forest land-cover types contained in those hot spots. Perforated forest, defined as a 0.09-ha unit of forest that is located at the center of a 7.29-ha neighborhood containing 60-99% forest with relatively low connectivity, was mapped over the eastern United States by using land-cover maps with roads superimposed. Statistically significant (P forest) were then located by using a spatial scan statistic. Hot spots were widely distributed and covered 20.4% of the total area of the 10 ecological provinces examined, but 50.1% of the total hot-spot area was concentrated in only two provinces. In the central part of the study area, more than 90% of the forest edge in hot spots was attributed to anthropogenic land-cover types, whereas in the northern and southern parts it was more often associated with semi-natural land cover such as herbaceous wetlands.

  12. Integrating sustainable hunting in biodiversity protection in Central Africa: hot spots, weak spots, and strong spots.

    Directory of Open Access Journals (Sweden)

    John E Fa

    Full Text Available Wild animals are a primary source of protein (bushmeat for people living in or near tropical forests. Ideally, the effect of bushmeat harvests should be monitored closely by making regular estimates of offtake rate and size of stock available for exploitation. However, in practice, this is possible in very few situations because it requires both of these aspects to be readily measurable, and even in the best case, entails very considerable time and effort. As alternative, in this study, we use high-resolution, environmental favorability models for terrestrial mammals (N = 165 in Central Africa to map areas of high species richness (hot spots and hunting susceptibility. Favorability models distinguish localities with environmental conditions that favor the species' existence from those with detrimental characteristics for its presence. We develop an index for assessing Potential Hunting Sustainability (PHS of each species based on their ecological characteristics (population density, habitat breadth, rarity and vulnerability, weighted according to restrictive and permissive assumptions of how species' characteristics are combined. Species are classified into five main hunting sustainability classes using fuzzy logic. Using the accumulated favorability values of all species, and their PHS values, we finally identify weak spots, defined as high diversity regions of especial hunting vulnerability for wildlife, as well as strong spots, defined as high diversity areas of high hunting sustainability potential. Our study uses relatively simple models that employ easily obtainable data of a species' ecological characteristics to assess the impacts of hunting in tropical regions. It provides information for management by charting the geography of where species are more or less likely to be at risk of extinction from hunting.

  13. Integrating sustainable hunting in biodiversity protection in Central Africa: hot spots, weak spots, and strong spots.

    Science.gov (United States)

    Fa, John E; Olivero, Jesús; Farfán, Miguel Ángel; Márquez, Ana Luz; Vargas, Juan Mario; Real, Raimundo; Nasi, Robert

    2014-01-01

    Wild animals are a primary source of protein (bushmeat) for people living in or near tropical forests. Ideally, the effect of bushmeat harvests should be monitored closely by making regular estimates of offtake rate and size of stock available for exploitation. However, in practice, this is possible in very few situations because it requires both of these aspects to be readily measurable, and even in the best case, entails very considerable time and effort. As alternative, in this study, we use high-resolution, environmental favorability models for terrestrial mammals (N = 165) in Central Africa to map areas of high species richness (hot spots) and hunting susceptibility. Favorability models distinguish localities with environmental conditions that favor the species' existence from those with detrimental characteristics for its presence. We develop an index for assessing Potential Hunting Sustainability (PHS) of each species based on their ecological characteristics (population density, habitat breadth, rarity and vulnerability), weighted according to restrictive and permissive assumptions of how species' characteristics are combined. Species are classified into five main hunting sustainability classes using fuzzy logic. Using the accumulated favorability values of all species, and their PHS values, we finally identify weak spots, defined as high diversity regions of especial hunting vulnerability for wildlife, as well as strong spots, defined as high diversity areas of high hunting sustainability potential. Our study uses relatively simple models that employ easily obtainable data of a species' ecological characteristics to assess the impacts of hunting in tropical regions. It provides information for management by charting the geography of where species are more or less likely to be at risk of extinction from hunting.

  14. Integrating Sustainable Hunting in Biodiversity Protection in Central Africa: Hot Spots, Weak Spots, and Strong Spots

    Science.gov (United States)

    Fa, John E.; Olivero, Jesús; Farfán, Miguel Ángel; Márquez, Ana Luz; Vargas, Juan Mario; Real, Raimundo; Nasi, Robert

    2014-01-01

    Wild animals are a primary source of protein (bushmeat) for people living in or near tropical forests. Ideally, the effect of bushmeat harvests should be monitored closely by making regular estimates of offtake rate and size of stock available for exploitation. However, in practice, this is possible in very few situations because it requires both of these aspects to be readily measurable, and even in the best case, entails very considerable time and effort. As alternative, in this study, we use high-resolution, environmental favorability models for terrestrial mammals (N = 165) in Central Africa to map areas of high species richness (hot spots) and hunting susceptibility. Favorability models distinguish localities with environmental conditions that favor the species' existence from those with detrimental characteristics for its presence. We develop an index for assessing Potential Hunting Sustainability (PHS) of each species based on their ecological characteristics (population density, habitat breadth, rarity and vulnerability), weighted according to restrictive and permissive assumptions of how species' characteristics are combined. Species are classified into five main hunting sustainability classes using fuzzy logic. Using the accumulated favorability values of all species, and their PHS values, we finally identify weak spots, defined as high diversity regions of especial hunting vulnerability for wildlife, as well as strong spots, defined as high diversity areas of high hunting sustainability potential. Our study uses relatively simple models that employ easily obtainable data of a species' ecological characteristics to assess the impacts of hunting in tropical regions. It provides information for management by charting the geography of where species are more or less likely to be at risk of extinction from hunting. PMID:25372705

  15. Models and methods for hot spot safety work

    DEFF Research Database (Denmark)

    Vistisen, Dorte

    2002-01-01

    is the task of improving road safety through alterations of the geometrical and environmental characteristics of the existing road network. The presently applied models and methods in hot spot safety work on the Danish road network were developed about two decades ago, when data was more limited and software...... and statistical methods less developed. The purpose of this thesis is to contribute to improving "State of the art" in Denmark. Basis for the systematic hot spot safety work are the models describing the variation in accident counts on the road network. In the thesis hierarchical models disaggregated on time......Despite the fact that millions DKK each year are spent on improving roadsafety in Denmark, funds for traffic safety are limited. It is therefore vital to spend the resources as effectively as possible. This thesis is concerned with the area of traffic safety denoted "hot spot safety work", which...

  16. Models and methods for hot spot safety work

    DEFF Research Database (Denmark)

    Vistisen, Dorte

    2002-01-01

    is the task of improving road safety through alterations of the geometrical and environmental characteristics of the existing road network. The presently applied models and methods in hot spot safety work on the Danish road network were developed about two decades ago, when data was more limited and software...... and statistical methods less developed. The purpose of this thesis is to contribute to improving "State of the art" in Denmark. Basis for the systematic hot spot safety work are the models describing the variation in accident counts on the road network. In the thesis hierarchical models disaggregated on time......Despite the fact that millions DKK each year are spent on improving roadsafety in Denmark, funds for traffic safety are limited. It is therefore vital to spend the resources as effectively as possible. This thesis is concerned with the area of traffic safety denoted "hot spot safety work", which...

  17. Analysis of structural hot-spot stress in orthotropic plates

    Institute of Scientific and Technical Information of China (English)

    Chen Ce; Ji Bohai; Xu Hanjiang

    2012-01-01

    On the basis of the actual steel deck structure of Taizhou Bridge, this paper carries out hot-spot stress analysis on some key spots by using the finite element model which simulates local structure of orthotropic steel bridge decks. A finite element model is established for local structure of orthotropic steel bridge decks, and in the analysis of linear elasticity of the structure, face load is employed to simulate the loads from vehicle wheels. Analysis results show that main stresses are relatively heavy at the joints between diaphragm plates, top plates and U-shaped ribs and the joints between diaphragm plates and U-shaped ribs. These joints shall be regarded as key points for hot-spot stress analysis. Different mesh densities are adopted in the finite element model and the main stresses at different hot spots are contrasted and linear extrapolation is carried out using extrapolation formulae. Results show that different mesh densities have different influences on the hot-spot stresses at the welded seams of U-shaped ribs. These influences shall be considered in calculation and analysis.

  18. Plasmonic electromagnetic hot spots temporally addressed by photoinduced molecular displacement.

    Energy Technology Data Exchange (ETDEWEB)

    Juan, M. L.; Plain, J.; Bachelot, R.; Vial, A.; Royer, P.; Gray, S. K.; Montgomery, J. M.; Wiederrecht, G. P.; Univ. de Technologie de Troyes

    2009-04-23

    We report the observation of temporally varying electromagnetic hot spots in plasmonic nanostructures. Changes in the field amplitude, position, and spatial features are induced by embedding plasmonic silver nanorods in the photoresponsive azo-polymer. This polymer undergoes cis?trans isomerization and wormlike transport within resonant optical fields, producing a time-varying local dielectric environment that alters the locations where electromagnetic hot spots are produced. Finite-difference time-domain and Monte Carlo simulations that model the induced field and corresponding material response are presented to aid in the interpretation of the experimental results. Evidence for propagating plasmons induced at the ends of the rods is also presented.

  19. Whole-genome sequencing in autism identifies hot spots for de novo germline mutation

    DEFF Research Database (Denmark)

    Michaelson, Jacob J.; Shi, Yujian; Gujral, Madhusudan

    2012-01-01

    De novo mutation plays an important role in autism spectrum disorders (ASDs). Notably, pathogenic copy number variants (CNVs) are characterized by high mutation rates. We hypothesize that hypermutability is a property of ASD genes and may also include nucleotide-substitution hot spots. We...

  20. Correlated wounded hot spots in proton-proton interactions

    Science.gov (United States)

    Albacete, Javier L.; Petersen, Hannah; Soto-Ontoso, Alba

    2017-06-01

    We investigate the effect of nontrivial spatial correlations between proton constituents, considered in this work to be gluonic hot spots, on the initial conditions of proton-proton collisions from ISR to Large Hadron Collider energies, i.e., √{s }=52.6 , 7000, and 13 000 GeV. The inclusion of these correlations is motivated by their fundamental role in the description of a recently observed new feature of p p scattering at √{s }=7 TeV, the hollowness effect. Our analysis relies on a Monte Carlo Glauber approach including fluctuations in the hot spot positions and their entropy deposition in the transverse plane. We explore both the energy dependence and the effect of spatial correlations on the number of wounded hot spots, their spatial distribution, and the eccentricities, ɛn, of the initial state geometry of the collision. In minimum bias collisions we find that the inclusion of short-range repulsive correlations between the hot spots reduces the value of the eccentricity (ɛ2) and the triangularity (ɛ3). In turn, upon considering only the events with the highest entropy deposition, i.e., the ultracentral ones, the probability of having larger ɛ2 ,3 increases significantly in the correlated scenario. Finally, the eccentricities show a quite mild energy dependence.

  1. Variability of Jupiter's Five-Micron Hot Spot Inventory

    Science.gov (United States)

    Yanamandra-Fisher, Padma A.; Orton, G. S.; Wakefield, L.; Rogers, J. H.; Simon-Miller, A. A.; Boydstun, K.

    2012-01-01

    Global upheavals on Jupiter involve changes in the albedo of entire axisymmetric regions, lasting several years, with the last two occurring in 1989 and 2006. Against this backdrop of planetary-scale changes, discrete features such as the Great Red Spot (GRS), and other vortices exhibit changes on shorter spatial- and time-scales. We track the variability of the discrete equatorial 5-micron hot spots, semi-evenly spaced in longitude and confined to a narrow latitude band centered at 6.5degN (southern edge of the North Equatorial Belt, NEB), abundant in Voyager images. Tantalizingly similar patterns were observed in the visible (bright plumes and blue-gray regions), where reflectivity in the red is anti-correlated with 5-microns thermal radiance. Ortiz et al. (1998, GRL, 103) characterized the latitude and drift rates of the hot spots, including the descent of the Galileo probe at the southern edge of a 5-micron hot spot, as the superposition of equatorial Rossby waves, with phase speeds between 99 - 103m/s, relative to System III. We note that the high 5-micron radiances correlate well but not perfectly with high 8.57-micron radiances. Because the latter are modulated primarily by changes in the upper ammonia (NH3) ice cloud opacity, this correlation implies that changes in the ammonia ice cloud field may be responsible for the variability seen in the 5-m maps. During the NEB fade (2011 - early 2012), however, these otherwise ubiquitous features were absent, an atmospheric state not seen in decades. The ongoing NEB revival indicates nascent 5-m hot spots as early as April 2012, with corresponding visible dark spots. Their continuing growth through July 2012 indicates the possit.le re-establishment of Rossby waves. The South Equatorial Belt (SEB) and NEB revivals began similarly with an instability that developed into a major outbreak, and many similarities in the observed propagation of clear regions.

  2. Extracting Hot spots of Topics from Time Stamped Documents.

    Science.gov (United States)

    Chen, Wei; Chundi, Parvathi

    2011-07-01

    Identifying time periods with a burst of activities related to a topic has been an important problem in analyzing time-stamped documents. In this paper, we propose an approach to extract a hot spot of a given topic in a time-stamped document set. Topics can be basic, containing a simple list of keywords, or complex. Logical relationships such as and, or, and not are used to build complex topics from basic topics. A concept of presence measure of a topic based on fuzzy set theory is introduced to compute the amount of information related to the topic in the document set. Each interval in the time period of the document set is associated with a numeric value which we call the discrepancy score. A high discrepancy score indicates that the documents in the time interval are more focused on the topic than those outside of the time interval. A hot spot of a given topic is defined as a time interval with the highest discrepancy score. We first describe a naive implementation for extracting hot spots. We then construct an algorithm called EHE (Efficient Hot Spot Extraction) using several efficient strategies to improve performance. We also introduce the notion of a topic DAG to facilitate an efficient computation of presence measures of complex topics. The proposed approach is illustrated by several experiments on a subset of the TDT-Pilot Corpus and DBLP conference data set. The experiments show that the proposed EHE algorithm significantly outperforms the naive one, and the extracted hot spots of given topics are meaningful.

  3. Venusian "hot spots": physical phenomenon and its quantification.

    Science.gov (United States)

    Goncharov, V P; Gryanik, V M; Pavlov, V I

    2002-12-01

    An overall picture of the Venusian hot spots phenomenon is considered in the framework of the simplest conceptual models that admit the solutions in the form of steadily rotating "hot" vortices. Model assumptions take into account only those features of the middle atmosphere in the polar region of Venus that are supported by observational data and are essential for understanding the physical mechanism initiating similar vortices. The problem is analyzed in the framework of both the pointlike and petal-like models of cyclostrophic vortices. Interpretation of these models as an upper and lower bound of a complete theory allows one to find the region of existence of the regimes responsible for the Venusian hot spots and also to establish and assess numerically conditions under which such vortices can be formed. The emphasis is on a comparison of the theoretically established results with the observational data.

  4. Dissection of a circumscribed recombination hot spot in HIV-1 after a single infectious cycle.

    Science.gov (United States)

    Galetto, Román; Giacomoni, Véronique; Véron, Michel; Negroni, Matteo

    2006-02-03

    Recombination is a major source of genetic heterogeneity in the human immunodeficiency virus type 1 (HIV-1) population. The main mechanism responsible for the generation of recombinant viruses is a process of copy choice between the two copies of genomic RNA during reverse transcription. We previously identified, after a single cycle of infection of cells in culture, a recombination hot spot within the gp120 gene, corresponding to the top portion of a RNA hairpin. Here, we determine that the hot region is circumscribed to 18 nucleotides located in the descending strand of the stem, following the sense of reverse transcription. Three factors appeared to be important, albeit at different extents, for the high rate of recombination observed in this region. The position of the hot sequence in the context of the RNA structure appears crucial, because changing its location within this structure triggered differences in recombination up to 20-fold. Another pivotal factor is the presence of a perfectly identical sequence between donor and acceptor RNA in the region of transfer, because single or double nucleotide differences in the hot spot were sufficient to almost completely abolish recombination in the region. Last, the primary structure of the hot region also influenced recombination, although with effects only in the 2-3-fold range. Altogether, these results provide the first molecular dissection of a hot spot in infected cells and indicate that several factors contribute to the generation of a site of preferential copy choice.

  5. Magnetic hot-spots in hollow silicon cylinders

    Science.gov (United States)

    Baryshnikova, K. V.; Evlyukhin, A. B.; Shalin, A. S.

    2016-08-01

    Silicon nanoparticles can possess magnetic Mie-resonant response in the visible and near infrared wavelength ranges. In this paper, we consider numerically the features of magnetic hot-spots realized inside silicon nanocylinders at the conditions of the optical magnetic resonances, and show that the intensity of the magnetic field inside nanoparticles with a coaxial through hole can be much stronger than the intensity of incident light waves.

  6. Thermal imaging of hot spots in nanostructured microstripes

    Science.gov (United States)

    Saïdi, E.; Lesueur, J.; Aigouy, L.; Labéguerie-Egéa, J.; Mortier, M.

    2010-03-01

    By scanning thermal microscopy, we study the behavior of nanostructured metallic microstripes heated by Joule effect. Regularly spaced indentations have been made along the thin film stripe in order to create hot spots. For the designed stripe geometry, we observe that heat remains confined in the wire and in particular at shrinkage points within ~1μm2. Thermal maps have been obtained with a good lateral resolution (< 300nm) and a good temperature sensitivity (~1K).

  7. Climate impacts on global hot spots of marine biodiversity

    Science.gov (United States)

    Ramírez, Francisco; Afán, Isabel; Davis, Lloyd S.; Chiaradia, André

    2017-01-01

    Human activities drive environmental changes at scales that could potentially cause ecosystem collapses in the marine environment. We combined information on marine biodiversity with spatial assessments of the impacts of climate change to identify the key areas to prioritize for the conservation of global marine biodiversity. This process identified six marine regions of exceptional biodiversity based on global distributions of 1729 species of fish, 124 marine mammals, and 330 seabirds. Overall, these hot spots of marine biodiversity coincide with areas most severely affected by global warming. In particular, these marine biodiversity hot spots have undergone local to regional increasing water temperatures, slowing current circulation, and decreasing primary productivity. Furthermore, when we overlapped these hot spots with available industrial fishery data, albeit coarser than our estimates of climate impacts, they suggest a worrying coincidence whereby the world’s richest areas for marine biodiversity are also those areas mostly affected by both climate change and industrial fishing. In light of these findings, we offer an adaptable framework for determining local to regional areas of special concern for the conservation of marine biodiversity. This has exposed the need for finer-scaled fishery data to assist in the management of global fisheries if the accumulative, but potentially preventable, effect of fishing on climate change impacts is to be minimized within areas prioritized for marine biodiversity conservation. PMID:28261659

  8. Distinguishing black holes and wormholes with orbiting hot spots

    Science.gov (United States)

    Li, Zilong; Bambi, Cosimo

    2014-07-01

    The supermassive black hole candidates at the center of every normal galaxy might be wormholes created in the early Universe and connecting either two different regions of our Universe or two different universes in a multiverse model. Indeed, the origin of these supermassive objects is not well understood; topological nontrivial structures like wormholes are allowed both in general relativity and in alternative theories of gravity, and current observations cannot rule out such a possibility. In a few years, the VLTI instrument GRAVITY will have the capability to image blobs of plasma orbiting near the innermost stable circular orbit of SgrA*, the supermassive black hole candidate in the Milky Way. The secondary image of a hot spot orbiting around a wormhole is substantially different from that of a hot spot around a black hole, because the photon capture sphere of the wormhole is much smaller. The radius of the photon capture sphere is independent of the hot spot model, and therefore its possible detection, which is observationally challenging but not out of reach, can unambiguously test if the center of our Galaxy harbors a wormhole rather than a black hole.

  9. Hot Spots and Persistence of Nitrate in Aquifers Across Scales

    Directory of Open Access Journals (Sweden)

    Dipankar Dwivedi

    2016-01-01

    Full Text Available Nitrate-N (NO3 -- N is one of the most pervasive contaminants in groundwater. Nitrate in groundwater exhibits long-term behavior due to complex interactions at multiple scales among various geophysical factors, such as sources of nitrate-N, characteristics of the vadose zone and aquifer attributes. To minimize contamination of nitrate-N in groundwater, it is important to estimate hot spots (>10 mg/L of NO3 -- N, trends and persistence of nitrate-N in groundwater. To analyze the trends and persistence of nitrate-N in groundwater at multiple spatio-temporal scales, we developed and used an entropy-based method along with the Hurst exponent in two different hydrogeologic settings: the Trinity and Ogallala Aquifers in Texas at fine (2 km × 2 km, intermediate (10 km × 10 km and coarse (100 km × 100 km scales. Results show that nitrate-N exhibits long-term persistence at the intermediate and coarse scales. In the Trinity Aquifer, overall mean nitrate-N has declined with a slight increase in normalized marginal entropy (NME over each decade from 1940 to 2008; however, the number of hot spots has increased over time. In the Ogallala Aquifer, overall mean nitrate-N has increased with slight moderation in NME since 1940; however, the number of hot spots has significantly decreased for the same period at all scales.

  10. Hot spot mix in ICF implosions on the NIF

    Science.gov (United States)

    Ma, Tammy

    2016-10-01

    In the quest to achieve ignition through the inertial confinement fusion scheme, one of the critical challenges is to drive a symmetric implosion at high velocity without hydrodynamic instabilities becoming detrimental. These instabilities, primarily at the ablation front and the fuel-ablator interface, can cause mix of the higher-Z shell into the hot spot, resulting in increased radiation loss and thus reduced temperature and neutron yield. To quantify the level of mix, we developed a model that infers the level of hot spot contamination using the ratio of the enhanced x-ray production relative to the neutron yield. Applying this methodology to the full ensemble of indirect-drive National Ignition Facility (NIF) cryogenically layered DT implosions provides insight on the sensitivity of performance to the level of ablator-hot spot mix. In particular, the improvement seen with the High Foot design can be primarily attributed to a reduction in ablation-front instability mix that enabled the implosions to be pushed to higher velocity and performance. This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344, Lawrence Livermore National Security, LLC.

  11. Real-time satellite monitoring of volcanic hot spots

    Science.gov (United States)

    Harris, Andrew J. L.; Flynn, Luke P.; Dean, Ken; Pilger, Eric; Wooster, Martin; Okubo, Chris; Mouginis-Mark, Peter; Garbeil, Harold; Thornber, Carl; De la Cruz-Reyna, Servando; Rothery, Dave; Wright, Robert

    Direct satellite data reception at high temporal frequencies and automated processing enable near-real-time, near-continuous thermal monitoring of volcanoes. We review what has been achieved in terms of turning this capability into real-time tools of use to volcano monitoring agencies. Current capabilities focus on 2 instruments: the advanced very high resolution radiometer (AVHRR) and the Geostationary Operational Environmental Satellite (GOES) imager. Collection of lO AVHRR images per day covering Alaska, the Aleutians, and Kamchatka allows routine, on-reception analysis of volcanic hot spots across this region. Data collected between 1996 and 1998 detected 302 hot spots due to lava flows, lava domes, pyroclastic flows, fumaroles, and geothermally heated lakes at 12 different volcanoes. Information was used for hazard mitigation by the Alaskan Volcano Observatory. GOES provides data for North and South American volcanoes every 15-30 minutes. Automated processing allows eruption information and alerts to be posted on the Internet within 15-60 minutes of reception. We use June 1998 to demonstrate the frequency of data acquisition. During this month 2879 GOES images were collected from which 14,832 sub-images of 6 active volcanoes were processed. Although 82% (12,200) of these sub-images were cloud covered, hot spots were still evident on 11% (1634) of the sub-images. Analysis of GOES data for 1998 identified hot spots due to (1) lava flows at Kilauea and Cerro Azul, (2) dome extrusion and explosive activity at Lascar, Popocatepetl, Colima and Pacaya, and (3) dome cooling and collapse at Soufriere Hills. We were also able to suggest that reports of lava flow activity at Cerro Negro were false. This information was supplied to, and used by, various agencies whose task it is to monitor these volcanoes. Global thermal monitoring will become a reality with the launch of the Earth Observing System's moderate resolution imaging spectrometer (MODIS). An automated thermal

  12. Variation in the Deep Gas Composition in Hot Spots on Jupiter

    Science.gov (United States)

    Bjoraker, Gordon; de Pater, Imke; Wong, Michael H.; Adamkovics, Mate; Hewagama, Tilak; Hesman, Brigette

    2015-11-01

    We used CSHELL on NASA’s Infrared Telescope Facility and NIRSPEC on the Keck telescope in the last two years to spectrally resolve line profiles of CH3D, NH3, PH3, and H2O in 5-micron Hot Spots on Jupiter. The profile of the CH3D lines at 4.66 microns is very broad in both NEB and SEB Hot Spots due to collisions with up to 8 bars of H2, where unit optical depth occurs due to collision-induced H2 opacity. The extreme width of these CH3D features implies that the Hot Spots that we observed do not have significant cloud opacity for P > 2 bars. We retrieved NH3, PH3, and gaseous H2O within Hot Spots in both the NEB and SEB. We had dry nights on Mauna Kea and a sufficient Doppler shift to detect H2O. We will compare line wings to derive H2O profiles in the 2 to 6-bar region. NEB Hot Spots are depleted in NH3 with respect to adjacent regions. Interestingly, SEB Hot Spots exhibit stronger NH3 absorption than NEB Hot Spots. In addition, SEB Hot Spots have very similar 5-micron spectra as neighboring longitudes in the SEB, implying similar deep gas composition. The dynamical origin of SEB Hot Spots is much less studied than that of NEB Hot Spots, so our observations of gas composition in both regions may constrain mechanisms for forming Hot Spots.

  13. Scientists Spot Genes Behind Crohn's, Ulcerative Colitis

    Science.gov (United States)

    ... medlineplus.gov/news/fullstory_166957.html Scientists Spot Genes Behind Crohn's, Ulcerative Colitis Large study finds key ... Researchers say they've come closer to pinpointing genes linked with inflammatory bowel diseases such as Crohn's ...

  14. Hot spots and hot moments in riparian zones: Potential for improved water quality management

    Science.gov (United States)

    Philippe Vidon; Craig Allan; Douglas Burns; Tim P. Duval; Noel Gurwick; Shreeram Inamdar; Richard Lowrance; Judy Okay; Durelle Scott; Stephen Sebestyen

    2010-01-01

    Biogeochemical and hydrological processes in riparian zones regulate contaminant movement to receiving waters and often mitigate the impact of upland sources of contaminants on water quality. These heterogeneous processes have recently been conceptualized as "hot spots and moments" of retention, degradation, or production. Nevertheless, studies investigating...

  15. Current-induced forces and hot spots in biased nanojunctions.

    Science.gov (United States)

    Lü, Jing-Tao; Christensen, Rasmus B; Wang, Jian-Sheng; Hedegård, Per; Brandbyge, Mads

    2015-03-06

    We investigate theoretically the interplay of current-induced forces (CIFs), Joule heating, and heat transport inside a current-carrying nanoconductor. We find that the CIFs, due to the electron-phonon coherence, can control the spatial heat dissipation in the conductor. This yields a significant asymmetric concentration of excess heating (hot spot) even for a symmetric conductor. When coupled to the electrode phonons, CIFs drive different phonon heat flux into the two electrodes. First-principles calculations on realistic biased nanojunctions illustrate the importance of the effect.

  16. Protein hot spots at bio-nano interfaces

    Directory of Open Access Journals (Sweden)

    Gerald F. Audette

    2011-07-01

    Full Text Available Nanotechnology has influenced the direction of research across the sciences, medicine, and engineering. Carbon nanotubes (CNTs and, more recently, protein nanotubes (PNTs and protein-inorganic nanocomposites have received considerable attention due to their unique nanostructures that can be utilized as a scaffold to house proteins or create nanowires. A shift towards protein-inorganic interactions has numerous applications from biosensors to biofuel cells and bio-based nanodevices. We examine several systems where protein hot spots, the active domains on proteins and the interactive dynamics in them, play a critical role in the interactions at the interface of these unique systems.

  17. Current-Induced Forces and Hot Spots in Biased Nanojunctions

    DEFF Research Database (Denmark)

    Lu, Jing Tao; Christensen, Rasmus Bjerregaard; Wang, Jian-Sheng;

    2015-01-01

    We investigate theoretically the interplay of current-induced forces (CIFs), Joule heating, and heat transport inside a current-carrying nanoconductor. We find that the CIFs, due to the electron-phonon coherence, can control the spatial heat dissipation in the conductor. This yields a significant...... asymmetric concentration of excess heating (hot spot) even for a symmetric conductor. When coupled to the electrode phonons, CIFs drive different phonon heat flux into the two electrodes. First-principles calculations on realistic biased nanojunctions illustrate the importance of the effect....

  18. Probing binding hot spots at protein-RNA recognition sites.

    Science.gov (United States)

    Barik, Amita; Nithin, Chandran; Karampudi, Naga Bhushana Rao; Mukherjee, Sunandan; Bahadur, Ranjit Prasad

    2016-01-29

    We use evolutionary conservation derived from structure alignment of polypeptide sequences along with structural and physicochemical attributes of protein-RNA interfaces to probe the binding hot spots at protein-RNA recognition sites. We find that the degree of conservation varies across the RNA binding proteins; some evolve rapidly compared to others. Additionally, irrespective of the structural class of the complexes, residues at the RNA binding sites are evolutionary better conserved than those at the solvent exposed surfaces. For recognitions involving duplex RNA, residues interacting with the major groove are better conserved than those interacting with the minor groove. We identify multi-interface residues participating simultaneously in protein-protein and protein-RNA interfaces in complexes where more than one polypeptide is involved in RNA recognition, and show that they are better conserved compared to any other RNA binding residues. We find that the residues at water preservation site are better conserved than those at hydrated or at dehydrated sites. Finally, we develop a Random Forests model using structural and physicochemical attributes for predicting binding hot spots. The model accurately predicts 80% of the instances of experimental ΔΔG values in a particular class, and provides a stepping-stone towards the engineering of protein-RNA recognition sites with desired affinity.

  19. Probing binding hot spots at protein–RNA recognition sites

    Science.gov (United States)

    Barik, Amita; Nithin, Chandran; Karampudi, Naga Bhushana Rao; Mukherjee, Sunandan; Bahadur, Ranjit Prasad

    2016-01-01

    We use evolutionary conservation derived from structure alignment of polypeptide sequences along with structural and physicochemical attributes of protein–RNA interfaces to probe the binding hot spots at protein–RNA recognition sites. We find that the degree of conservation varies across the RNA binding proteins; some evolve rapidly compared to others. Additionally, irrespective of the structural class of the complexes, residues at the RNA binding sites are evolutionary better conserved than those at the solvent exposed surfaces. For recognitions involving duplex RNA, residues interacting with the major groove are better conserved than those interacting with the minor groove. We identify multi-interface residues participating simultaneously in protein–protein and protein–RNA interfaces in complexes where more than one polypeptide is involved in RNA recognition, and show that they are better conserved compared to any other RNA binding residues. We find that the residues at water preservation site are better conserved than those at hydrated or at dehydrated sites. Finally, we develop a Random Forests model using structural and physicochemical attributes for predicting binding hot spots. The model accurately predicts 80% of the instances of experimental ΔΔG values in a particular class, and provides a stepping-stone towards the engineering of protein–RNA recognition sites with desired affinity. PMID:26365245

  20. Hot spot-derived shock initiation phenomena in heterogeneous nitromethane

    Energy Technology Data Exchange (ETDEWEB)

    Dattelbaum, Dana M [Los Alamos National Laboratory; Sheffield, Stephen A [Los Alamos National Laboratory; Stahl, David B [Los Alamos National Laboratory; Dattelbaum, Andrew M [Los Alamos National Laboratory

    2009-01-01

    The addition of solid silica particles to gelled nitromethane offers a tractable model system for interrogating the role of impedance mismatches as one type of hot spot 'seed' on the initiation behaviors of explosive formulations. Gas gun-driven plate impact experiments are used to produce well-defined shock inputs into nitromethane-silica mixtures containing size-selected silica beads at 6 wt%. The Pop-plots or relationships between shock input pressure and rundistance (or time)-to-detonation for mixtures containing small (1-4 {micro}m) and large (40 {micro}m) beads are presented. Overall, the addition of beads was found to influence the shock sensitivity of the mixtures, with the smaller beads being more sensitizing than the larger beads, lowering the shock initiation threshold for the same run distance to detonation compared with neat nitromethane. In addition, the use of embedded electromagnetic gauges provides detailed information pertaining to the mechanism of the build-up to detonation and associated reactive flow. Of note, an initiation mechanism characteristic of homogeneous liquid explosives, such as nitromethane, was observed in the nitromethane-40 {micro}m diameter silica samples at high shock input pressures, indicating that the influence of hot spots on the initiation process was minimal under these conditions.

  1. Magnetised Accretion Discs in Kerr Spacetimes II: Hot Spots

    CERN Document Server

    García, Federico; Johannsen, Tim

    2015-01-01

    Context. Quasi-periodic variability has been observed in a number of X-ray binaries harboring black hole candidates. In general relativity, black holes are uniquely described by the Kerr metric and, according to the cosmic censorship conjecture, curvature singularities always have to be clothed by an event horizon. Aims. In this paper, we study the effect of an external magnetic field on the observed light curves of orbiting hot spots in thin accretion discs around Kerr black holes and naked singularities. Methods. We employ a ray-tracing algorithm to calculate the light curves and power spectra of such hot spots as seen by a distant observer for uniform and dipolar magnetic field configurations assuming a weak coupling between the magnetic field and the disc matter. Results. We show that the presence of an external dipolar magnetic field leads to potentially observable modifications of these signals for both Kerr black holes and naked singularities, while an external uniform magnetic field has practically no...

  2. Distinguishing black holes and wormholes with orbiting hot spots

    CERN Document Server

    Li, Zilong

    2014-01-01

    The supermassive black hole candidates at the center of every normal galaxy might be wormholes created in the early Universe and connecting either two different regions of our Universe or two different universes in a Multiverse model. Indeed, the origin of these supermassive objects is not well understood, topological non-trivial structures like wormholes are allowed both in general relativity and in alternative theories of gravity, and current observations cannot rule out such a possibility. In a few years, the VLTI instrument GRAVITY will have the capability to image blobs of plasma orbiting near the innermost stable circular orbit of SgrA$^*$, the supermassive black hole candidate in the Milky Way. The secondary image of a hot spot orbiting around a wormhole is substantially different from the one of a hot spot around a black hole, because the photon capture sphere of the wormhole is much smaller, and its detection could thus test if the center of our Galaxy harbors a wormhole rather then a black hole.

  3. Microblog Hot Spot Mining Based on PAM Probabilistic Topic Model

    Directory of Open Access Journals (Sweden)

    Zheng Yaxin

    2015-01-01

    Full Text Available Microblogs are short texts carried with limited information, which will increase the difficulty of topic mining. This paper proposes the use of PAM (Pachinko Allocation Model probabilistic topic model to extract the generative model of text’s implicit theme for microblog hot spot mining. First, three categories of microblog and the main contribution of this paper are illustrated. Second, for there are four topic models which are respectively explained, the PAM model is introduced in detail in terms of how to generate a document, the accuracy of document classification and the topic correlation in PAM. Finally, MapReduce is described. For the number of microblogs is huge as well as the number of contactors, the totally number of words is relatively small. With MapReduce, microblogs data are split by contactor, document-topic count matrix and contactor-topic count matrix can be locally stored while the word-topic count matrix must be globally stored. Thus, the hot spot mining can be achieved on the basis of PAM probabilistic topic model.

  4. Detecting traffic hot spots using vehicle tracking data

    Science.gov (United States)

    Xu, Zhimin; Lin, Zhiyong; Zhou, Cheng; Huang, Changqing

    2016-03-01

    Vehicle tracking data for thousands of urban vehicles and the availability of digital map provide urban planners unprecedented opportunities for better understanding urban transportation. In this paper, we aim to detect traffic hot spots on urban road networks using vehicle tracking data. Our approach first proposes an integrated map-matching algorithm based on the road buffer and vehicle driving direction, to find out which road segment the vehicle is travelling on. Then, we estimate travel speed by calculating the average the speed of every vehicle on a certain road segment, which indicates traffic status, and create the spatial weights matrices based on the connectivity of road segments, which expresses the spatial dependence between each road segment. Finally, the measure of global and local spatial autocorrelation is used to evaluate the spatial distribution of the traffic condition and reveal the traffic hot spots on the road networks. Experiments based on the taxi tracking data and urban road network data from Wuhan have been performed to validate the detection effectiveness.

  5. Hot spots and active longitudes: Organization of solar activity as a probe of the interior

    Science.gov (United States)

    Bai, Taeil; Hoeksema, J. Todd; Scherrer, Phil H.

    1995-01-01

    In order to investigate how solar activity is organized in longitude, major solar flares, large sunspot groups, and large scale photospheric magnetic field strengths were analyzed. The results of these analyses are reported. The following results are discussed: hot spots, initially recognized as areas of high concentration of major flares, are the preferred locations for the emergence of big sunspot groups; double hot spots appear in pairs that rotate at the same rate separated by about 180 deg in longitude, whereas, single hot spots have no such companions; the northern and southern hemispheres behave differently in organizing solar activity in longitude; the lifetime of hot spots range from one to several solar cycles; a hot spot is not always active throughout its lifetime, but goes through dormant periods; and hot spots with different rotational periods coexist in the same hemisphere during the same solar cycle.

  6. Human imprinted chromosomal regions are historical hot-spots of recombination.

    Directory of Open Access Journals (Sweden)

    Ionel Sandovici

    2006-07-01

    Full Text Available Human recombination rates vary along the chromosomes as well as between the two sexes. There is growing evidence that epigenetic factors may have an important influence on recombination rates, as well as on crossover position. Using both public database analysis and wet-bench approaches, we revisited the relationship between increased rates of meiotic recombination and genome imprinting. We constructed metric linkage disequilibrium (LD maps for all human chromosomal regions known to contain one or more imprinted genes. We show that imprinted regions contain significantly more LD units (LDU and have significantly more haplotype blocks of smaller sizes than flanking nonimprinted regions. There is also an excess of hot-spots of recombination at imprinted regions, and this is likely to do with the presence of imprinted genes, per se. These findings indicate that imprinted chromosomal regions are historical "hot-spots" of recombination. We also demonstrate, by direct segregation analysis at the 11p15.5 imprinted region, that there is remarkable agreement between sites of meiotic recombination and steps in LD maps. Although the increase in LDU/Megabase at imprinted regions is not associated with any significant enrichment for any particular sequence class, major sequence determinants of recombination rates seem to differ between imprinted and control regions. Interestingly, fine-mapping of recombination events within the most male meiosis-specific recombination hot-spot of Chromosome 11p15.5 indicates that many events may occur within or directly adjacent to regions that are differentially methylated in somatic cells. Taken together, these findings support the involvement of a combination of specific DNA sequences and epigenetic factors as major determinants of hot-spots of recombination at imprinted chromosomal regions.

  7. Cold spots in neonatal incubators are hot spots for microbial contamination.

    Science.gov (United States)

    de Goffau, Marcus C; Bergman, Klasien A; de Vries, Hendrik J; Meessen, Nico E L; Degener, John E; van Dijl, Jan Maarten; Harmsen, Hermie J M

    2011-12-01

    Thermal stability is essential for the survival and well-being of preterm neonates. This is achieved in neonatal incubators by raising the ambient temperature and humidity to sufficiently high levels. However, potentially pathogenic microorganisms also can thrive in such warm and humid environments. We therefore investigated whether the level of microbial contamination (i.e., the bacterial load) inside neonatal incubators can be predicted on the basis of their average temperature and relative humidity settings, paying special attention to local temperature differences. Swab samples were taken from the warmest and coldest spots found within Caleo incubators, and these were plated to determine the number of microbial CFU per location. In incubators with high average temperature (≥ 34°C) and relative humidity (≥ 60%) values, the level of microbial contamination was significantly higher at cold spots than at hot spots. This relates to the fact that the local equilibrium relative humidity at cold spots is sufficiently high to sustain microbial growth. The abundance of staphylococci, which are the main causative agents of late-onset sepsis in preterm neonates, was found to be elevated significantly in cold areas. These findings can be used to improve basic incubator hygiene.

  8. Modeling Hot-Spot Contributions in Shocked High Explosives at the Mesoscale

    Energy Technology Data Exchange (ETDEWEB)

    Harrier, Danielle [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-08-12

    When looking at performance of high explosives, the defects within the explosive become very important. Plastic bonded explosives, or PBXs, contain voids of air and bonder between the particles of explosive material that aid in the ignition of the explosive. These voids collapse in high pressure shock conditions, which leads to the formation of hot spots. Hot spots are localized high temperature and high pressure regions that cause significant changes in the way the explosive material detonates. Previously hot spots have been overlooked with modeling, but now scientists are realizing their importance and new modeling systems that can accurately model hot spots are underway.

  9. Hot spots in energetic materials generated by infrared and ultrasound, detected by thermal imaging microscopy.

    Science.gov (United States)

    Chen, Ming-Wei; You, Sizhu; Suslick, Kenneth S; Dlott, Dana D

    2014-02-01

    We have observed and characterized hot spot formation and hot-spot ignition of energetic materials (EM), where hot spots were created by ultrasonic or long-wavelength infrared (LWIR) exposure, and were detected by high-speed thermal microscopy. The microscope had 15-20 μm spatial resolution and 8.3 ms temporal resolution. LWIR was generated by a CO2 laser (tunable near 10.6 μm or 28.3 THz) and ultrasound by a 20 kHz acoustic horn. Both methods of energy input created spatially homogeneous energy fields, allowing hot spots to develop spontaneously due to the microstructure of the sample materials. We observed formation of hot spots which grew and caused the EM to ignite. The EM studied here consisted of composite solids with 1,3,5-trinitroperhydro-1,3,5-triazine crystals and polymer binders. EM simulants based on sucrose crystals in binders were also examined. The mechanisms of hot spot generation were different with LWIR and ultrasound. With LWIR, hot spots were most efficiently generated within the EM crystals at LWIR wavelengths having longer absorption depths of ∼25 μm, suggesting that hot spot generation mechanisms involved localized absorbing defects within the crystals, LWIR focusing in the crystals or LWIR interference in the crystals. With ultrasound, hot spots were primarily generated in regions of the polymer binder immediately adjacent to crystal surfaces, rather than inside the EM crystals.

  10. Vascular design for reducing hot spots and stresses

    Science.gov (United States)

    Rocha, L. A. O.; Lorente, S.; Bejan, A.

    2014-05-01

    This paper is a proposal to embed tree-shaped vasculatures in a wall designed such that the wall withstands without excessive hot spots and peak stresses the intense heating and pressure that impinge on it. The vasculature is a quilt of square-shaped panels, each panel having a tree vasculature that connects the center with the perimeter. The vascular designs for volumetric cooling can be complemented by the shaping and distributing of channels for maximum strength and thermal performance at the same time. Numerical simulations of heat flow and thermal stresses in three directions show that it is possible to determine the optimal geometric features of configurations with radial channels and trees with radial and one level of bifurcations. The global performance is evaluated in terms of the overall thermal resistance and peak von Mises stresses. The dendritic design is superior under the studied thermal condition.

  11. Accurate prediction of hot spot residues through physicochemical characteristics of amino acid sequences

    KAUST Repository

    Chen, Peng

    2013-07-23

    Hot spot residues of proteins are fundamental interface residues that help proteins perform their functions. Detecting hot spots by experimental methods is costly and time-consuming. Sequential and structural information has been widely used in the computational prediction of hot spots. However, structural information is not always available. In this article, we investigated the problem of identifying hot spots using only physicochemical characteristics extracted from amino acid sequences. We first extracted 132 relatively independent physicochemical features from a set of the 544 properties in AAindex1, an amino acid index database. Each feature was utilized to train a classification model with a novel encoding schema for hot spot prediction by the IBk algorithm, an extension of the K-nearest neighbor algorithm. The combinations of the individual classifiers were explored and the classifiers that appeared frequently in the top performing combinations were selected. The hot spot predictor was built based on an ensemble of these classifiers and to work in a voting manner. Experimental results demonstrated that our method effectively exploited the feature space and allowed flexible weights of features for different queries. On the commonly used hot spot benchmark sets, our method significantly outperformed other machine learning algorithms and state-of-the-art hot spot predictors. The program is available at http://sfb.kaust.edu.sa/pages/software.aspx. © 2013 Wiley Periodicals, Inc.

  12. Dynamic programming-based hot spot identification approach for pedestrian crashes.

    Science.gov (United States)

    Medury, Aditya; Grembek, Offer

    2016-08-01

    Network screening techniques are widely used by state agencies to identify locations with high collision concentration, also referred to as hot spots. However, most of the research in this regard has focused on identifying highway segments that are of concern to automobile collisions. In comparison, pedestrian hot spot detection has typically focused on analyzing pedestrian crashes in specific locations, such as at/near intersections, mid-blocks, and/or other crossings, as opposed to long stretches of roadway. In this context, the efficiency of the some of the widely used network screening methods has not been tested. Hence, in order to address this issue, a dynamic programming-based hot spot identification approach is proposed which provides efficient hot spot definitions for pedestrian crashes. The proposed approach is compared with the sliding window method and an intersection buffer-based approach. The results reveal that the dynamic programming method generates more hot spots with a higher number of crashes, while providing small hot spot segment lengths. In comparison, the sliding window method is shown to suffer from shortcomings due to a first-come-first-serve approach vis-à-vis hot spot identification and a fixed hot spot window length assumption.

  13. The R304X mutation of the Aryl hydrocarbon receptor Interacting Protein gene in familial isolated pituitary adenomas: mutational Hot-Spot or founder effect?

    OpenAIRE

    Occhi, G.; Jaffrain-Rea, M. L.; Trivellin, G.; Albiger, N; Ceccato, F.; De Menis, E.; Angelini, M.; Ferasin, S; Beckers, Albert; F. Mantero; Scaroni, C.

    2010-01-01

    Background: Mutations in the Aryl hydrocarbon receptor Interacting Protein (AIP) gene have been described in about 15% of kindreds with Familial Isolated Pituitary Adenomas (FIPA) and in a minority of early onset sporadic pituitary adenomas (PA). Among the AIP mutations reported so far, the R304X (AIPR304X) represents, together with the "Finnish mutation" Q14X, the most common one. Methods: Three AIPR304X Italian families, including a newly reported kindred, have been genotyped for 12 genetic...

  14. Evidence for the Daxiawan as a hot spot in the Earth

    Institute of Scientific and Technical Information of China (English)

    汤懋苍; 钟大赉; 李文华; 冯松

    1999-01-01

    From the point of view of application, the definition of hot spot was given first. The solid geophysical evidence suggested that the Daxiawan (great gorge) in Yarlung Zangbo River should be a hot spot, which is a special region with high ground temperature, low density, low magnetism, negative gravity abnormality, frequent earthquake and strong tectonics. According to the data available, the center of the hot spot was preliminarily decided. The difference between the ground surface and air temperature in January is negatively correlated with the distance from the hot spot center to the meteorological station. According to the ground surface energy balance equation, the geothermal flux in the center of hot spot is about 25 W/m~2. Further analysis suggested that Daxiawan is a pilot region of climate change as well as the region of the heaviest rainfall and highest latitude of tropical forest in the world.

  15. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot".

    Directory of Open Access Journals (Sweden)

    Sharon E Johnatty

    2010-07-01

    Full Text Available We hypothesized that variants in genes expressed as a consequence of interactions between ovarian cancer cells and the host micro-environment could contribute to cancer susceptibility. We therefore used a two-stage approach to evaluate common single nucleotide polymorphisms (SNPs in 173 genes involved in stromal epithelial interactions in the Ovarian Cancer Association Consortium (OCAC. In the discovery stage, cases with epithelial ovarian cancer (n=675 and controls (n=1,162 were genotyped at 1,536 SNPs using an Illumina GoldenGate assay. Based on Positive Predictive Value estimates, three SNPs-PODXL rs1013368, ITGA6 rs13027811, and MMP3 rs522616-were selected for replication using TaqMan genotyping in up to 3,059 serous invasive cases and 8,905 controls from 16 OCAC case-control studies. An additional 18 SNPs with Pper-alleleor=0.5. However genotypes at TERT rs7726159 were associated with ovarian cancer risk in the smaller, five-study replication study (Pper-allele=0.03. Combined analysis of the discovery and replication sets for this TERT SNP showed an increased risk of serous ovarian cancer among non-Hispanic whites [adj. ORper-allele 1.14 (1.04-1.24 p=0.003]. Our study adds to the growing evidence that, like the 8q24 locus, the telomerase reverse transcriptase locus at 5p15.33, is a general cancer susceptibility locus.

  16. Identification of retinal transformation hot spots in developing Drosophila epithelia.

    Directory of Open Access Journals (Sweden)

    Claire L Salzer

    Full Text Available BACKGROUND: The retinal determination (RD network is an evolutionarily conserved regulatory circuit that governs early events in the development of eyes throughout the animal kingdom. Ectopic expression of many members of this network leads to the transformation of non-retinal epithelia into eye tissue. An often-overlooked observation is that only particular cell-populations within a handful of tissues are capable of having their primary developmental instructions superseded and overruled. METHODOLOGY/PRELIMINARY FINDINGS: Here we confirm that indeed, only a discrete number of cell populations within the imaginal discs that give rise to the head, antenna, legs, wings and halteres have the cellular plasticity to have their developmental fates altered. In contrast to previous reports, we find that all transformable cell populations do not lie within the TGFbeta or Hedgehog signaling domains. Additionally neither signaling cascade alone is sufficient for non-retinal cell types to be converted into retinal tissue. The transformation "hot spots" that we have identified appear to coincide with several previously defined transdetermination "weak spots", suggesting that ectopic eye formation is less the result of one network overriding the orders of another, as previously thought, but rather is the physical manifestation of redirecting cell populations of enormous cellular plasticity. We also demonstrate that the initiation of eye formation in non-retinal tissues occurs asynchronously compared to that of the normal eye suggesting that retinal development is not under the control of a global developmental clock. CONCLUSIONS/SIGNIFICANCE: We conclude that the subregions of non-retinal tissues that are capable of supporting eye formation represent specialized cell-populations that have a different level of plasticity than other cells within these tissues and may be the founder cells of each tissue.

  17. Association of poly-purine/poly-pyrimidine sequences with meiotic recombination hot spots

    Directory of Open Access Journals (Sweden)

    Pitt Joel PW

    2006-07-01

    Full Text Available Abstract Background Meiotic recombination events have been found to concentrate in 1–2.5 kilo base regions, but these recombination hot spots do not share a consensus sequence and why they occur at specific sites is not fully understood. Some previous evidence suggests that poly-purine/poly-pyrimidine (poly-pu/py tracts (PPTs, a class of sequence with distinctive biochemical properties, could be involved in recombination, but no general association of PPTs with meiotic recombination hot spots has previously been reported. Results We used computational methods to investigate in detail the relationship between PPTs and hot spots. We show statistical associations of PPT frequency with hot spots of meiotic recombination initiating lesions, double-strand breaks, in the genome of the yeast S. cerevisiae and with experimentally well characterized human meiotic recombination hot spots. Supporting a possible role of poly-pu/py-rich sequences in hot spot recombination, we also found that all three single nucleotide polymorphisms previously shown to be associated with human hot spot activity changes occur within sequence contexts of 14 bp or longer that are 85% or more poly-pu/py and at least 70% G/C. These polymorphisms are all close to the hot spot mid points. Comparing the sequences of experimentally characterized human hot spots with the orthologous regions of the chimpanzee genome previously shown not to contain hot spots, we found that in all five cases in which comparisons for the hot spot central regions are possible with publicly available sequence data, there are differences near the human hot spot mid points within sequences 14 bp or longer consisting of more than 80% poly-pu/py and at least 50% G/C. Conclusion Our results, along with previous evidence for the unique biochemical properties and recombination-stimulating potential of poly-pu/py-rich sequences, suggest that the possible functional involvement of this type of sequence in meiotic

  18. Identification of biogeochemical hot spots using time-lapse hydrogeophysics

    Science.gov (United States)

    Franz, T. E.; Loecke, T.; Burgin, A.

    2016-12-01

    The identification and monitoring of biogeochemical hot spots and hot moments is difficult using point based sampling techniques and sensors. Without proper monitoring and accounting of water, energy, and trace gas fluxes it is difficult to assess the environmental footprint of land management practices. One key limitation is optimal placement of sensors/chambers that adequately capture the point scale fluxes and thus a reasonable integration to landscape scale flux. In this work we present time-lapse hydrogeophysical imaging at an old agricultural field converted into a wetland mitigation bank near Dayton, Ohio. While the wetland was previously instrumented with a network of soil sensors and surface chambers to capture a suite of state variables and fluxes, we hypothesize that time-lapse hydrogeophysical imaging is an underutilized and critical reconnaissance tool for effective network design and landscape scaling. Here we combine the time-lapse hydrogeophysical imagery with the multivariate statistical technique of Empirical Orthogonal Functions (EOF) in order to isolate the spatial and temporal components of the imagery. Comparisons of soil core information (e.g. soil texture, soil carbon) from around the study site and organized within like spatial zones reveal statistically different mean values of soil properties. Moreover, the like spatial zones can be used to identify a finite number of future sampling locations, evaluation of the placement of existing sensors/chambers, upscale/downscale observations, all of which are desirable techniques for commercial use in precision agriculture. Finally, we note that combining the EOF analysis with continuous monitoring from point sensors or remote sensing products may provide a robust statistical framework for scaling observations through time as well as provide appropriate datasets for use in landscape biogeochemical models.

  19. Multi-stable dissipative structures pinned to dual hot spots

    CERN Document Server

    Tsang, Cheng Hou; Chow, Kwok Wing

    2011-01-01

    We analyze the formation of one-dimensional localized patterns in a nonlinear dissipative medium including a set of two narrow "hot spots" (HSs), which carry the linear gain, local potential, cubic self-interaction, and cubic loss, while the linear loss acts in the host medium. This system can be realized, as a spatial-domain one, in optics, and also in Bose-Einstein condensates of quasi-particles in solid-state settings. Recently, exact solutions were found for localized modes pinned to the single HS represented by the delta-function. The present paper reports analytical and numerical solutions for coexisting two- and multi-peak modes, which may be symmetric or antisymmetric with respect to the underlying HS pair. Stability of the modes is explored through simulations of their perturbed evolution. The sign of the cubic nonlinearity plays a crucial role: in the case of the self-focusing, only the fundamental symmetric and antisymmetric modes, with two local peaks tacked to the HSs, and no additional peaks bet...

  20. Wafer hot spot identification through advanced photomask characterization techniques

    Science.gov (United States)

    Choi, Yohan; Green, Michael; McMurran, Jeff; Ham, Young; Lin, Howard; Lan, Andy; Yang, Richer; Lung, Mike

    2016-10-01

    As device manufacturers progress through advanced technology nodes, limitations in standard 1-dimensional (1D) mask Critical Dimension (CD) metrics are becoming apparent. Historically, 1D metrics such as Mean to Target (MTT) and CD Uniformity (CDU) have been adequate for end users to evaluate and predict the mask impact on the wafer process. However, the wafer lithographer's process margin is shrinking at advanced nodes to a point that the classical mask CD metrics are no longer adequate to gauge the mask contribution to wafer process error. For example, wafer CDU error at advanced nodes is impacted by mask factors such as 3-dimensional (3D) effects and mask pattern fidelity on subresolution assist features (SRAFs) used in Optical Proximity Correction (OPC) models of ever-increasing complexity. These items are not quantifiable with the 1D metrology techniques of today. Likewise, the mask maker needs advanced characterization methods in order to optimize the mask process to meet the wafer lithographer's needs. These advanced characterization metrics are what is needed to harmonize mask and wafer processes for enhanced wafer hot spot analysis. In this paper, we study advanced mask pattern characterization techniques and their correlation with modeled wafer performance.

  1. Distinct Rayleigh scattering from hot spot mutant p53 proteins reveals cancer cells.

    Science.gov (United States)

    Jun, Ho Joon; Nguyen, Anh H; Kim, Yeul Hong; Park, Kyong Hwa; Kim, Doyoun; Kim, Kyeong Kyu; Sim, Sang Jun

    2014-07-23

    The scattering of light redirects and resonances when an electromagnetic wave interacts with electrons orbits in the hot spot core protein and oscillated electron of the gold nanoparticles (AuNP). This report demonstrates convincingly that resonant Rayleigh scattering generated from hot spot mutant p53 proteins is correspondence to cancer cells. Hot spot mutants have unique local electron density changes that affect specificity of DNA binding affinity compared with wild types. Rayleigh scattering changes introduced by hot-spot mutations were monitored by localized surface plasmon resonance (LSPR) shift changes. The LSPR λmax shift for hot-spot mutants ranged from 1.7 to 4.2 nm for mouse samples and from 0.64 nm to 2.66 nm for human samples, compared to 9.6 nm and 15 nm for wild type and mouse and human proteins, respectively with a detection sensitivity of p53 concentration at 17.9 nM. It is interesting that hot-spot mutants, which affect only interaction with DNA, launches affinitive changes as considerable as wild types. These changes propose that hot-spot mutants p53 proteins can be easily detected by local electron density alterations that disturbs the specificity of DNA binding of p53 core domain on the surface of the DNA probed-nanoplasmonic sensor.

  2. Hot spot detection for breast cancer in Ki-67 stained slides: image dependent filtering approach

    Science.gov (United States)

    Niazi, M. Khalid Khan; Downs-Kelly, Erinn; Gurcan, Metin N.

    2014-03-01

    We present a new method to detect hot spots from breast cancer slides stained for Ki67 expression. It is common practice to use centroid of a nucleus as a surrogate representation of a cell. This often requires the detection of individual nuclei. Once all the nuclei are detected, the hot spots are detected by clustering the centroids. For large size images, nuclei detection is computationally demanding. Instead of detecting the individual nuclei and treating hot spot detection as a clustering problem, we considered hot spot detection as an image filtering problem where positively stained pixels are used to detect hot spots in breast cancer images. The method first segments the Ki-67 positive pixels using the visually meaningful segmentation (VMS) method that we developed earlier. Then, it automatically generates an image dependent filter to generate a density map from the segmented image. The smoothness of the density image simplifies the detection of local maxima. The number of local maxima directly corresponds to the number of hot spots in the breast cancer image. The method was tested on 23 different regions of interest images extracted from 10 different breast cancer slides stained with Ki67. To determine the intra-reader variability, each image was annotated twice for hot spots by a boardcertified pathologist with a two-week interval in between her two readings. A computer-generated hot spot region was considered a true-positive if it agrees with either one of the two annotation sets provided by the pathologist. While the intra-reader variability was 57%, our proposed method can correctly detect hot spots with 81% precision.

  3. An Attribute Oriented Stimulate Algorithm For Detecting and Mapping Crime Hot Spots

    OpenAIRE

    Vijaykumar, M.; Chandrasekar, Dr. C.

    2011-01-01

    cCrime mapping is a very effective method for detecting high-crime-density areas known as hot spots. Crime hot spot is an area where the number of criminal or disorder events is larger than that in any other places, or an area where people have a higher risk of victimization. There are many theories and methods in common use by far. They explain different types of crime phenomena that occur at different geographic levels. The method which is used most widely for detecting crime hot spots is t...

  4. Features of surface enhanced Raman scattering in the systems with «hot spots»

    Directory of Open Access Journals (Sweden)

    Solovyeva E.V.

    2017-01-01

    Full Text Available In this work we demonstrate the features of SERS on the substrates with «hot spots» on the example of system «diaminostilbene - colloidal silver». We found that «hot spots» forming on aggregated nanoparticles exist on the metal substrates only at low concentration of ligand. This effect caused by the gradual filling of first monolayer by adsorbate molecules. Significantly higher enhancement factor is obtained for substrates with «hot spots», for which the participation of resonance processes in the formation of SERS signal is revealed also.

  5. Features of surface enhanced Raman scattering in the systems with «hot spots»

    Science.gov (United States)

    Solovyeva, E. V.; Khazieva, D. A.; Denisova, A. S.

    2016-12-01

    In this work we demonstrate the features of SERS on the substrates with «hot spots» on the example of system «diaminostilbene - colloidal silver». We found that «hot spots» forming on aggregated nanoparticles exist on the metal substrates only at low concentration of ligand. This effect caused by the gradual filling of first monolayer by adsorbate molecules. Significantly higher enhancement factor is obtained for substrates with «hot spots», for which the participation of resonance processes in the formation of SERS signal is revealed also.

  6. HOT SPOT RELIEF WITH EMBEDDED BEAM FOR CDMA SYSTEMS IN HAPS

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This paper proposes a novel micro/macro beam coverage scheme used in High Altitude Platform System (HAPS) Code Division Multiple Access (CDMA) systems. A relief of traffic burden in hot spot areas is achieved by embedding micro-beams into the macro-beams at the hot spot locations, together with appropriate power ratio control and user ratio control. The simulation results show that the hot spot problem can be relieved efficiently with the presented configuration, and a higher and more stable system capacity is expectable despite the variation of user distribution.

  7. Coral bleaching and ocean ''hot spots''

    Energy Technology Data Exchange (ETDEWEB)

    Goreau, T.J. (Global Coral Reef Alliance, Chappaqua, NY (United States)); Hayes, R.L. (Howard Univ., Washington, DC (United States). College of Medicine)

    1994-05-01

    Global sea-surface temperature maps show that mass coral-reef bleaching episodes between 1983 and 1991 followed positive anomalies more than 1 deg C above long-term monthly averages (''hot spots'') during the preceding warm season. Irregular formation, movement, and disappearance of hot spots make their detailed long-term prediction impossible, but they can be tracked in real time from satellite data. Monitoring of ocean hot spots and of coral bleaching is needed if the Framework Convention of Climate Change is to meet its goal of protecting the most temperature sensitive ecosystems. 47 refs, 3 figs

  8. Ring of nine Gamma Ray Burst overlap with the hot spot of my hypothesis

    Science.gov (United States)

    Cao, Dayong

    2016-03-01

    During 2004 to 2014, a symmetry axis and a cold spot (a structure of one billion light years across) of CMB were observed, and I supposed there is a hot spot, and there is a symmetry between the cold spot and the hot spot of CMB. http://www.dailymail.co.uk/sciencetech/article-2430415 http://meetings.aps.org/link/BAPS.2014.MAR.Y33.9 In 2015, a Ring of Nine Gamma Ray Burst (a structure of FIVE BILLION light years across) which is a part of structure of double helix and overlap with the hot spot was observed. http://www.dailymail.co.uk/sciencetech/article-3185193 The Ring of Nine Gamma Ray Burst could be explained by the hot spot. There is a balance systemic model with structure of double helix of the flat universe between cold spot and hot spot-a balance between stellar matter and dark massenergy (include dark matter and dark energy). The model can explain of the Hubble's redshift. There is a larger dark hole instead of the huge black hole of the center of the Milky Way galaxy, and a dark hole builds up a balance system with sun. This model should explain of the seasonal Extinctions. http://meetings.aps.org/link/BAPS.2015.APR.H14.8

  9. Two CpG mutational hot spots in the X-linked interleukin-2 receptor gamma chain gene (IL2RG) may cause 15% of all human severe combined immunodeficiency

    Energy Technology Data Exchange (ETDEWEB)

    Pepper, A.E.; Puck, J.M. [National Center for Human Genome Research, Bethseda, MD (United States); Liu, X. [Univ. of Pennsylvania School of Medicine, Philadelphia, PA (United States)

    1994-09-01

    Severe combined immunodeficiency (SCID), a syndrome of profoundly impaired cellular and humoral immune function, is caused by various autosomal gene defects, including adenosine deaminase (ADA) deficiency, as well as mutations in the X-linked IL2RG gene encoding the gamma chain of the lymphocyte receptor for IL-2. Mutational analysis of IL2RG was performed using genomic DNA from males with SCID referred from genetics and immunology centers. Single strand conformation polymorphisms (SSCP) were sought by PCR amplification of each of the 8 IL2RG exons using labelled flanking primers. Sequence of exons with aberrant SSCP detected a majority of unique deleterious IL2RG mutations in 30 unrelated SCID patients. However, multiple mutations were seen at CpG dinucleotides, known to be C{yields}T transversion sites. cDNA 690-691 in exon 5 was mutated in 4 patients, 1 patient with each of the C{sub 690}{yields}T causing an Arg{yields}Cys substitution, and 1 with G{sub 691}{yields}A causing Arg{yields}His. Two other patients had SCID caused by a single mutation in IL2RG exon 7. This C{sub 879}{yields}T, also in a CpG, changed an Arg to STOP, resulting in loss of the SH2-related intracellular domain. In addition to our patients, 1 patient with each of the C{sub 690} and the C{sub 879} mutations have been reported by others, giving an overall incidence of 20% from our lab and 21% from all reported IL2RG SCID mutations. While ADA defects account for approximately 15% of SCID, a striking male SCID predominance suggest up to 70% of the cases are X-linked, due to IL2RG mutation. Thus, screening for mutations at the 2 CpG hot spots we have found in IL2RG can identify the genotype of as many SCID cases as are found by ADA testing.

  10. Prediction of protein interaction hot spots using rough set-based multiple criteria linear programming.

    Science.gov (United States)

    Chen, Ruoying; Zhang, Zhiwang; Wu, Di; Zhang, Peng; Zhang, Xinyang; Wang, Yong; Shi, Yong

    2011-01-21

    Protein-protein interactions are fundamentally important in many biological processes and it is in pressing need to understand the principles of protein-protein interactions. Mutagenesis studies have found that only a small fraction of surface residues, known as hot spots, are responsible for the physical binding in protein complexes. However, revealing hot spots by mutagenesis experiments are usually time consuming and expensive. In order to complement the experimental efforts, we propose a new computational approach in this paper to predict hot spots. Our method, Rough Set-based Multiple Criteria Linear Programming (RS-MCLP), integrates rough sets theory and multiple criteria linear programming to choose dominant features and computationally predict hot spots. Our approach is benchmarked by a dataset of 904 alanine-mutated residues and the results show that our RS-MCLP method performs better than other methods, e.g., MCLP, Decision Tree, Bayes Net, and the existing HotSprint database. In addition, we reveal several biological insights based on our analysis. We find that four features (the change of accessible surface area, percentage of the change of accessible surface area, size of a residue, and atomic contacts) are critical in predicting hot spots. Furthermore, we find that three residues (Tyr, Trp, and Phe) are abundant in hot spots through analyzing the distribution of amino acids.

  11. Controlling Surface-plasmon-polariton Launching with Hot Spot Cylindrical Waves in a Metallic Slit Structure

    CERN Document Server

    Yao, Wenjie; Chen, Jianjun; Gong, Qihuang

    2015-01-01

    Plasmonic nanostructures, which are used to generate surface plasmon polaritions (SPPs), always involve sharp corners where the charges can accumulate. This can result in strong localized electromagnetic fields at the metallic corners, forming hot spots. The influence of the hot spots on the propagating SPPs are investigated theoretically and experimentally in a metallic slit structure. It is found that the electromagnetic fields radiated from the hot spots, termed as the hot spot cylindrical wave (HSCW), can greatly manipulate the SPP launching in the slit structure. The physical mechanism behind the manipulation of the SPP launching with the HSCW is explicated by a semi-analytic model. By using the HSCW, unidirectional SPP launching is experimentally realized in an ultra-small metallic step-slit structure. The HSCW bridges the localized surface plasmons and the propagating surface plasmons in an integrated platform and thus may pave a new route to the design of plasmonic devices and circuits.

  12. Fireball ejection from a molten hot spot to air by localized microwaves.

    Science.gov (United States)

    Dikhtyar, Vladimir; Jerby, Eli

    2006-02-03

    A phenomenon of fireball ejection from hot spots in solid materials (silicon, germanium, glass, ceramics, basalt, etc.) to the atmosphere is presented. The hot spot is created in the substrate material by the microwave-drill mechanism [Jerby, Science 298, 587 (2002)10.1126/science.1077062]. The vaporized drop evolved from the hot spot is blown up, and forms a stable fireball buoyant in the air. The experimental observations of fireball ejection from silicate hot spots are referred to the Abrahamson-Dinniss theory [Nature (London) 403, 519 (2000)10.1038/35000525] suggesting a mechanism for ball-lightning initiation in nature. The fireballs observed in our experiments tend to absorb the available microwave power entirely, similarly to the plasmon resonance effect in submicron wavelengths [Nie and Emory, Science 275, 1102 (1997)10.1126/science.275.5303.1102].

  13. Hot spot formation and stagnation properties in simulations of direct-drive NIF implosions

    Science.gov (United States)

    Schmitt, Andrew J.; Obenschain, Stephen P.

    2016-05-01

    We investigate different proposed methods of increasing the hot spot energy and radius in inertial confinement fusion implosions. In particular, shock mistiming (preferentially heating the inner edge of the target's fuel) and increasing the initial vapor gas density are investigated as possible control mechanisms. We find that only the latter is effective in substantially increasing the hot spot energy and dimensions while achieving ignition. In all cases an increase in the hot spot energy is accompanied by a decrease in the hot spot energy density (pressure) and both the yield and the gain of the target drop substantially. 2D simulations of increased vapor density targets predict an increase in the robustness of the target with respect to surface perturbations but are accompanied by significant yield degradation.

  14. Hot Spots and Hot Times: Wildlife Road Mortality in a Regional Conservation Corridor

    Science.gov (United States)

    Garrah, Evelyn; Danby, Ryan K.; Eberhardt, Ewen; Cunnington, Glenn M.; Mitchell, Scott

    2015-10-01

    Strategies to reduce wildlife road mortality have become a significant component of many conservation efforts. However, their success depends on knowledge of the temporal and spatial patterns of mortality. We studied these patterns along the 1000 Islands Parkway in Ontario, Canada, a 37 km road that runs adjacent to the St. Lawrence River and bisects the Algonquin-to-Adirondacks international conservation corridor. Characteristics of all vertebrate road kill were recorded during 209 bicycle surveys conducted from 2008 to 2011. We estimate that over 16,700 vertebrates are killed on the road from April to October each year; most are amphibians, but high numbers of birds, mammals, and reptiles were also found, including six reptiles considered at-risk in Canada. Regression tree analysis was used to assess the importance of seasonality, weather, and traffic on road kill magnitude. All taxa except mammals exhibited distinct temporal peaks corresponding to phases in annual life cycles. Variations in weather and traffic were only important outside these peak times. Getis-Ord analysis was used to identify spatial clusters of mortality. Hot spots were found in all years for all taxa, but locations varied annually. A significant spatial association was found between multiyear hot spots and wetlands. The results underscore the notion that multi-species conservation efforts must account for differences in the seasonality of road mortality among species and that multiple years of data are necessary to identify locations where the greatest conservation good can be achieved. This information can be used to inform mitigation strategies with implications for conservation at regional scales.

  15. Hot Spots and Hot Times: Wildlife Road Mortality in a Regional Conservation Corridor.

    Science.gov (United States)

    Garrah, Evelyn; Danby, Ryan K; Eberhardt, Ewen; Cunnington, Glenn M; Mitchell, Scott

    2015-10-01

    Strategies to reduce wildlife road mortality have become a significant component of many conservation efforts. However, their success depends on knowledge of the temporal and spatial patterns of mortality. We studied these patterns along the 1000 Islands Parkway in Ontario, Canada, a 37 km road that runs adjacent to the St. Lawrence River and bisects the Algonquin-to-Adirondacks international conservation corridor. Characteristics of all vertebrate road kill were recorded during 209 bicycle surveys conducted from 2008 to 2011. We estimate that over 16,700 vertebrates are killed on the road from April to October each year; most are amphibians, but high numbers of birds, mammals, and reptiles were also found, including six reptiles considered at-risk in Canada. Regression tree analysis was used to assess the importance of seasonality, weather, and traffic on road kill magnitude. All taxa except mammals exhibited distinct temporal peaks corresponding to phases in annual life cycles. Variations in weather and traffic were only important outside these peak times. Getis-Ord analysis was used to identify spatial clusters of mortality. Hot spots were found in all years for all taxa, but locations varied annually. A significant spatial association was found between multiyear hot spots and wetlands. The results underscore the notion that multi-species conservation efforts must account for differences in the seasonality of road mortality among species and that multiple years of data are necessary to identify locations where the greatest conservation good can be achieved. This information can be used to inform mitigation strategies with implications for conservation at regional scales.

  16. Modeling Hot Spot Motor Vehicle Theft Crime in Relation to Landuse and Settlement Patterns

    Directory of Open Access Journals (Sweden)

    Djaka Marwasta

    2004-01-01

    Full Text Available The crowd of Yogyakarta urban has impacted its surrounding area, including Depok sub district, which is indicated by the rising of physical development, for example education facilities and settlements. The progress does not only bring positive impact, but also negative impact for instance the rising of crime number i.e. motor vehicle robbery. The aims of this research are 1 mapping motor vehicle robbery data as the distribution map and identifying motor vehicle robbery hot spot base on distrbution map; and 2 studying the correlation of motor vehicle robbery hot spot with physical environment phenomena, i.e. land use type and settlement pattern. The research method consists of two parts; they are motor vehicle robbery cluster analysis and the relation of motor vehicle robbery and physical environment analysis. Motor vehicle robbery cluster analysis is using distribution data, which analyzes the distribution into motor vehicle robbery hot spot with nearest neighbor tehnique. Contingency coefficient and frequency distribution analysis is used to analyze the correlation of motor vehicle robbery hot spot and physical environment. Contingency coefficient is used to study the relation of motor vehicle robbery hot spot polygon with physical environment condition, whereas frequency distribution is used to study the distribution of motor vehicle robbery in the hot spot with physical environment condition. Physical environment which consists of land use type, housing density, house regularity pattern, and the average of building size, are obtained from interpretation of black and white panchromatic aerial photograph year 2000, in the scale 1 : 20.000. the most motor vehicle robbery hot spot is found on the settlement area, 68,3% from 378 motor vehicle robbery cases in the hot spot. The seond level is found on the education area (16.4%. The most motor vehicle hot spot in the settlement is found on the hight density and irregular settlement, which have big

  17. Avoiding Carbon Bed Hot Spots in Thermal Process Off-Gas Systems

    Energy Technology Data Exchange (ETDEWEB)

    Nick Soelberg; Joe Enneking

    2011-05-01

    Mercury has had various uses in nuclear fuel reprocessing and other nuclear processes, and so is often present in radioactive and mixed (radioactive and hazardous) wastes. Test programs performed in recent years have shown that mercury in off-gas streams from processes that treat radioactive wastes can be controlled using fixed beds of activated sulfur-impregnated carbon, to levels low enough to comply with air emission regulations such as the Hazardous Waste Combustor (HWC) Maximum Achievable Control Technology (MACT) standards. Carbon bed hot spots or fires have occurred several times during these tests, and also during a remediation of tanks that contained mixed waste. Hot spots occur when localized areas in a carbon bed become heated to temperatures where oxidation occurs. This heating typically occurs due to heat of absoption of gas species onto the carbon, but it can also be caused through external means such as external heaters used to heat the carbon bed vessel. Hot spots, if not promptly mitigated, can grow into bed fires. Carbon bed hot spots and fires must be avoided in processes that treat radioactive and mixed waste. Hot spots are detected by (a) monitoring in-bed and bed outlet gas temperatures, and (b) more important, monitoring of bed outlet gas CO concentrations. Hot spots are mitigated by (a) designing for appropriate in-bed gas velocity, for avoiding gas flow maldistribution, and for sufficient but not excessive bed depth, (b) appropriate monitoring and control of gas and bed temperatures and compositions, and (c) prompt implementation of corrective actions if bed hot spots are detected. Corrective actions must be implemented quickly if bed hot spots are detected, using a graded approach and sequence starting with corrective actions that are simple, quick, cause the least impact to the process, and are easiest to recover from.

  18. Analysis of microstructure-dependent shock dissipation and hot-spot formation in granular metalized explosive

    Science.gov (United States)

    Chakravarthy, Sunada; Gonthier, Keith A.

    2016-07-01

    Variations in the microstructure of granular explosives (i.e., particle packing density, size, shape, and composition) can affect their shock sensitivity by altering thermomechanical fields at the particle-scale during pore collapse within shocks. If the deformation rate is fast, hot-spots can form, ignite, and interact, resulting in burn at the macro-scale. In this study, a two-dimensional finite and discrete element technique is used to simulate and examine shock-induced dissipation and hot-spot formation within low density explosives (68%-84% theoretical maximum density (TMD)) consisting of large ensembles of HMX (C4H8N8O8) and aluminum (Al) particles (size ˜ 60 -360 μm). Emphasis is placed on identifying how the inclusion of Al influences effective shock dissipation and hot-spot fields relative to equivalent ensembles of neat/pure HMX for shocks that are sufficiently strong to eliminate porosity. Spatially distributed hot-spot fields are characterized by their number density and area fraction enabling their dynamics to be described in terms of nucleation, growth, and agglomeration-dominated phases with increasing shock strength. For fixed shock particle speed, predictions indicate that decreasing packing density enhances shock dissipation and hot-spot formation, and that the inclusion of Al increases dissipation relative to neat HMX by pressure enhanced compaction resulting in fewer but larger HMX hot-spots. Ensembles having bimodal particle sizes are shown to significantly affect hot-spot dynamics by altering the spatial distribution of hot-spots behind shocks.

  19. Avoiding Carbon Bed Hot Spots in Thermal Process Off-Gas Systems

    Energy Technology Data Exchange (ETDEWEB)

    Nick Soelberg; Joe Enneking

    2011-05-01

    Mercury has had various uses in nuclear fuel reprocessing and other nuclear processes, and so is often present in radioactive and mixed (radioactive and hazardous) wastes. Test programs performed in recent years have shown that mercury in off-gas streams from processes that treat radioactive wastes can be controlled using fixed beds of activated sulfur-impregnated carbon, to levels low enough to comply with air emission regulations such as the Hazardous Waste Combustor (HWC) Maximum Achievable Control Technology (MACT) standards. Carbon bed hot spots or fires have occurred several times during these tests, and also during a remediation of tanks that contained mixed waste. Hot spots occur when localized areas in a carbon bed become heated to temperatures where oxidation occurs. This heating typically occurs due to heat of absoption of gas species onto the carbon, but it can also be caused through external means such as external heaters used to heat the carbon bed vessel. Hot spots, if not promptly mitigated, can grow into bed fires. Carbon bed hot spots and fires must be avoided in processes that treat radioactive and mixed waste. Hot spots are detected by (a) monitoring in-bed and bed outlet gas temperatures, and (b) more important, monitoring of bed outlet gas CO concentrations. Hot spots are mitigated by (a) designing for appropriate in-bed gas velocity, for avoiding gas flow maldistribution, and for sufficient but not excessive bed depth, (b) appropriate monitoring and control of gas and bed temperatures and compositions, and (c) prompt implementation of corrective actions if bed hot spots are detected. Corrective actions must be implemented quickly if bed hot spots are detected, using a graded approach and sequence starting with corrective actions that are simple, quick, cause the least impact to the process, and are easiest to recover from.

  20. Understanding and controlling hot spots of crime: the importance of formal and informal social controls.

    Science.gov (United States)

    Weisburd, David; Groff, Elizabeth R; Yang, Sue-Ming

    2014-02-01

    Primary, secondary, and tertiary prevention programs that address opportunity or structural factors related to crime are usually delivered to entire cities, sections of cities or to specific neighborhoods, but our results indicate geographically targeting these programs to specific street segments may increase their efficacy. We link crime incidents to over 24,000 street segments (the two block faces on a street between two intersections) over a 16-year period, and identify distinct developmental patterns of crime at street segments using group-based trajectory analysis. One of these patterns, which we term chronic crime hot spots, includes just 1 % of street segments but is associated with 23 % of crime in the city during the study period. We then employ multinomial regression to identify the specific risk and protective factors that are associated with these crime hot spots. We find that both situational opportunities and social characteristics of places strongly distinguish chronic crime hot spots from areas with little crime. Our findings support recent efforts to decrease crime opportunities at crime hot spots through programs like hot spots policing, but they also suggest that social interventions directed at crime hot spots will be important if we are to do something about crime problems in the long run. We argue in concluding that micro level programs which focus crime prevention efforts on specific street segments have the potential to be less costly and more effective than those targeted at larger areas such as communities or neighborhoods.

  1. Protein-protein interface analysis and hot spots identification for chemical ligand design.

    Science.gov (United States)

    Chen, Jing; Ma, Xiaomin; Yuan, Yaxia; Pei, Jianfeng; Lai, Luhua

    2014-01-01

    Rational design for chemical compounds targeting protein-protein interactions has grown from a dream to reality after a decade of efforts. There are an increasing number of successful examples, though major challenges remain in the field. In this paper, we will first give a brief review of the available methods that can be used to analyze protein-protein interface and predict hot spots for chemical ligand design. New developments of binding sites detection, ligandability and hot spots prediction from the author's group will also be described. Pocket V.3 is an improved program for identifying hot spots in protein-protein interface using only an apo protein structure. It has been developed based on Pocket V.2 that can derive receptor-based pharmacophore model for ligand binding cavity. Given similarities and differences between the essence of pharmacophore and hot spots for guiding design of chemical compounds, not only energetic but also spatial properties of protein-protein interface are used in Pocket V.3 for dealing with protein-protein interface. In order to illustrate the capability of Pocket V.3, two datasets have been used. One is taken from ASEdb and BID having experimental alanine scanning results for testing hot spots prediction. The other is taken from the 2P2I database containing complex structures of protein-ligand binding at the original protein-protein interface for testing hot spots application in ligand design.

  2. Microwave interrogation of an air plasma plume as a model system for hot spots in explosives

    Science.gov (United States)

    Kane, Ronald J.; Tringe, Joseph W.; Klunder, Gregory L.; Baluyot, Emer V.; Densmore, John M.; Converse, Mark C.

    2017-01-01

    The evolution of hot spots within explosives is critical to understand for predicting how detonation waves form and propagate. However, it is challenging to observe hot spots directly because they are small (˜micron diameter), form quickly (much less than a microsecond), and many explosives of interest are optically opaque. Microwaves are well-suited to characterize hot spots because they readily penetrate most explosives. They also have sufficient temporal and spatial resolution to measure the coalescence of an ensemble of hot spots inside explosives. Here we employ 94 GHz microwaves to characterize the evolution of individual plasma plumes formed by laser ionization of air. We use interferometry to obtain plume diameter as a function of time. Although the plasma plumes are larger than individual hot spots in explosives, they expand rapidly and predictably, and their structure can be optically imaged. They are therefore useful model systems to establish the spatial and temporal limits of microwave interferometry (MI) for understanding more complex hot spot behavior in solid explosives.

  3. Identification of hot-spot residues in protein-protein interactions by computational docking

    Directory of Open Access Journals (Sweden)

    Fernández-Recio Juan

    2008-10-01

    Full Text Available Abstract Background The study of protein-protein interactions is becoming increasingly important for biotechnological and therapeutic reasons. We can define two major areas therein: the structural prediction of protein-protein binding mode, and the identification of the relevant residues for the interaction (so called 'hot-spots'. These hot-spot residues have high interest since they are considered one of the possible ways of disrupting a protein-protein interaction. Unfortunately, large-scale experimental measurement of residue contribution to the binding energy, based on alanine-scanning experiments, is costly and thus data is fairly limited. Recent computational approaches for hot-spot prediction have been reported, but they usually require the structure of the complex. Results We have applied here normalized interface propensity (NIP values derived from rigid-body docking with electrostatics and desolvation scoring for the prediction of interaction hot-spots. This parameter identifies hot-spot residues on interacting proteins with predictive rates that are comparable to other existing methods (up to 80% positive predictive value, and the advantage of not requiring any prior structural knowledge of the complex. Conclusion The NIP values derived from rigid-body docking can reliably identify a number of hot-spot residues whose contribution to the interaction arises from electrostatics and desolvation effects. Our method can propose residues to guide experiments in complexes of biological or therapeutic interest, even in cases with no available 3D structure of the complex.

  4. Identification and location of hot and cold spots of treated prevalence of depression in Catalonia (Spain

    Directory of Open Access Journals (Sweden)

    Salinas-Pérez José A

    2012-08-01

    Full Text Available Abstract Background Spatial analysis is a relevant set of tools for studying the geographical distribution of diseases, although its methods and techniques for analysis may yield very different results. A new hybrid approach has been applied to the spatial analysis of treated prevalence of depression in Catalonia (Spain according to the following descriptive hypotheses: 1 spatial clusters of treated prevalence of depression (hot and cold spots exist and, 2 these clusters are related to the administrative divisions of mental health care (catchment areas in this region. Methods In this ecological study, morbidity data per municipality have been extracted from the regional outpatient mental health database (CMBD-SMA for the year 2009. The second level of analysis mapped small mental health catchment areas or groups of municipalities covered by a single mental health community centre. Spatial analysis has been performed using a Multi-Objective Evolutionary Algorithm (MOEA which identified geographical clusters (hot spots and cold spots of depression through the optimization of its treated prevalence. Catchment areas, where hot and cold spots are located, have been described by four domains: urbanicity, availability, accessibility and adequacy of provision of mental health care. Results MOEA has identified 6 hot spots and 4 cold spots of depression in Catalonia. Our results show a clear spatial pattern where one cold spot contributed to define the exact location, shape and borders of three hot spots. Analysing the corresponding domain values for the identified hot and cold spots no common pattern has been detected. Conclusions MOEA has effectively identified hot/cold spots of depression in Catalonia. However these hot/cold spots comprised municipalities from different catchment areas and we could not relate them to the administrative distribution of mental care in the region. By combining the analysis of hot/cold spots, a better statistical and

  5. Prediction of hot spots in protein interfaces using a random forest model with hybrid features.

    Science.gov (United States)

    Wang, Lin; Liu, Zhi-Ping; Zhang, Xiang-Sun; Chen, Luonan

    2012-03-01

    Prediction of hot spots in protein interfaces provides crucial information for the research on protein-protein interaction and drug design. Existing machine learning methods generally judge whether a given residue is likely to be a hot spot by extracting features only from the target residue. However, hot spots usually form a small cluster of residues which are tightly packed together at the center of protein interface. With this in mind, we present a novel method to extract hybrid features which incorporate a wide range of information of the target residue and its spatially neighboring residues, i.e. the nearest contact residue in the other face (mirror-contact residue) and the nearest contact residue in the same face (intra-contact residue). We provide a novel random forest (RF) model to effectively integrate these hybrid features for predicting hot spots in protein interfaces. Our method can achieve accuracy (ACC) of 82.4% and Matthew's correlation coefficient (MCC) of 0.482 in Alanine Scanning Energetics Database, and ACC of 77.6% and MCC of 0.429 in Binding Interface Database. In a comparison study, performance of our RF model exceeds other existing methods, such as Robetta, FOLDEF, KFC, KFC2, MINERVA and HotPoint. Of our hybrid features, three physicochemical features of target residues (mass, polarizability and isoelectric point), the relative side-chain accessible surface area and the average depth index of mirror-contact residues are found to be the main discriminative features in hot spots prediction. We also confirm that hot spots tend to form large contact surface areas between two interacting proteins. Source data and code are available at: http://www.aporc.org/doc/wiki/HotSpot.

  6. 40 CFR 93.123 - Procedures for determining localized CO, PM10, and PM2.5 concentrations (hot-spot analysis).

    Science.gov (United States)

    2010-07-01

    ... ratio of future to current traffic and the ratio of future to current emission factors. (3) Hot-spot... CO, PM10, and PM2.5 concentrations (hot-spot analysis). 93.123 Section 93.123 Protection of... concentrations (hot-spot analysis). (a) CO hot-spot analysis. (1) The demonstrations required by §...

  7. Joule heating hot spot at high latitudes in the afternoon sector

    Science.gov (United States)

    Cai, L.; Aikio, A. T.; Milan, S. E.

    2016-07-01

    The afternoon Joule heating hot spot has been studied statistically by using the EISCAT Svalbard Radar (ESR) measurements at 75.4° Corrected Geomagnetic latitude (CGMLAT) and the OMNI solar wind data base. For a small subset of events, the Active Magnetosphere and Planetary Electrodynamics Response Experiment (AMPERE) field-aligned current distributions have been available. The main results are as follows. Afternoon Joule heating hot spots are associated with high values of ionospheric electric fields and slightly enhanced Pedersen conductances. The Joule heating hot spot values are larger in summer than in winter, which can be explained by the higher Pedersen conductances during summer than winter. The afternoon Joule heating hot spots are located close to the reversals of the large-scale field-aligned current systems. The most common location is close to the Region 1/Region 2 boundary and those events are associated with sunward convecting F region plasma. In a few cases, the hot spots take place close to the Region 1/Region 0 boundary and then the ionospheric plasma is convecting antisunward. The hot spots may occur both during slow (450 km/s) speed solar wind conditions. During slow-speed solar wind events, the dominant interplanetary magnetic field (IMF) direction is southward, which is the general requirement for the low-latitude magnetic merging at the dayside magnetopause. During high-speed solar wind, also northward IMF conditions appear, but those are associated with large values of the IMF |By| component, making again the dayside magnetopause merging possible. Finally, the measured afternoon hot spot Joule heating rates are not a linear function of the solar wind energy coupling function.

  8. What controls the distribution and tectono-magmatic features of oceanic hot spot volcanoes

    Science.gov (United States)

    Acocella, Valerio; Vezzoli, Luigina

    2016-04-01

    Hot spot oceanic volcanoes worldwide show significant deviations from the classic Hawaiian reference model; these mainly concern the distribution of edifices and overall tectono-magmatic features, as the development of the volcanic rift zones and extent of flank instability. Here we try to explain these deviations investigating and comparing the best-known hot spot oceanic volcanoes. At a general scale, these volcanoes show an age-distance progression ranging from focused to scattered. This is here explained as due to several independent factors, as the thermal or mechanical weakening of the plate (due to the lithosphere thickness or regional structures, respectively), or the plume structure. At a more detailed scale, hot spot volcanoes show recurrent features, including mafic shield edifices with summit caldera and volcanic rift zones, often at the head of an unstable flank. However, despite this recurrence, a widespread tectono-magmatic variability is often found. Here we show how this variability depends upon the magma supply and age of the oceanic crust (influencing the thickness of the overlying pelagic sediments). Well-developed rift zones and larger collapses are found on hot spot volcanoes with higher supply rate and older crust, as Hawaii and Canary Islands. Poorly-developed rift zones and limited collapses occur on hot spot volcanoes with lower supply rate and younger crust, as Easter Island and Ascension. Transitional features are observed at hot spots with intermediate productivity (Cape Verde, Reunion, Society Islands and, to a minor extent, the Azores), whereas the scarcity or absence of pelagic sediments may explain the lack of collapses and developed rift zones in the productive Galapagos hot spot.

  9. Hot spot(s) of the lung in technetium-99m albumin colloid liver-spleen scintigraphy: case report

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Brandenburg, S.; Coupal, J.J.; Sullivan, J.D.; Beeler, J.A.; Magoun, S.; Ryo, U.Y.

    1988-06-01

    The authors replaced /sup 99m/Tc albumin colloid for /sup 99m/Tc sulfur colloid as a radiopharmaceutical for liver-spleen imaging and found two instances of hot spot(s) in the lung. The preparation procedure of albumin colloid is easier and more convenient as compared to that of sulfur colloid. Whereas replacement of /sup 99m/Tc sulfur colloid by /sup 99m/Tc albumin colloid is inevitable, it should be emphasized that one should avoid blood withdrawal in the syringe containing albumin colloid to prevent formation of clot(s) during the venous puncture for /sup 99m/Tc albumin colloid.

  10. Hydrologic indicators of hot spots and hot moments of mercury methylation along river corridors

    Science.gov (United States)

    Singer, Michael; Harrison, Lee; Donovan, Patrick; Blum, Joel; Marvin-DiPasquale, Mark

    2016-04-01

    The biogeochemical cycling of metals and other contaminants river-floodplain corridors is controlled by microbial activity is often affected by dynamic redox conditions. Riverine flooding thus has the potential to affect speciation of redox-sensitive metals such as mercury (Hg). Therefore, flow history over a period of decades potentially holds information on past production of bioavailable Hg. We investigate this process within a Northern California river system that has a legacy of industrial-scale 19th century hydraulic gold mining. In the first known application of this methodology, we combine hydraulic modeling, measurements of Hg species in sediment and biota, and first-order calculations to assess the role of river floodplains in producing monomethylmercury (MMHg), which accumulates in local and migratory biota. We identify areas that represent 'hot spots' (frequently inundated areas of floodplains) and 'hot moments' (floodplain areas inundated for consecutive long periods). We show that the probability of MMHg production in each sector of the river system is dependent on the spatial patterns of overbank flow and drainage, which affect its long-term redox history. MMHg bioaccumulation within the aquatic food web may pose a major risk to humans and waterfowl that eat migratory salmonids, which are being encouraged to come up these rivers to spawn, and there appears to be no end to MMHg production under a regime of increasingly common large floods with extended duration. These findings identify river floodplains as periodic, temporary, yet important, loci of biogeochemical transformation in which contaminants may undergo change during limited periods of the historical hydrologic record. We suggest that inundation is the primary driver of MMHg production in river corridors and that the entire flow history must be analyzed in terms of magnitude and frequency of inundation in order to accurately assess biogeochemical risks, rather than merely highlighting the

  11. Genome-wide mapping of hot spots of DNA double-strand breaks in human cells as a tool for epigenetic studies and cancer genomics

    Directory of Open Access Journals (Sweden)

    N.A. Tchurikov

    2015-09-01

    Full Text Available Hot spots of DNA double-strand breaks (DSBs are associated with coordinated expression of genes in chromosomal domains (Tchurikov et al., 2011 [1]; 2013. These 50–150-kb DNA domains (denoted “forum domains” can be visualized by separation of undigested chromosomal DNA in pulsed-field agarose gels (Tchurikov et al., 1988; 1992 and used for genome-wide mapping of the DSBs that produce them. Recently, we described nine hot spots of DSBs in human rDNA genes and observed that, in rDNA units, the hot spots coincide with CTCF binding sites and H3K4me3 marks (Tchurikov et al., 2014, suggesting a role for DSBs in active transcription. Here we have used Illumina sequencing to map DSBs in chromosomes of human HEK293T cells, and describe in detail the experimental design and bioinformatics analysis of the data deposited in the Gene Expression Omnibus with accession number GSE53811 and associated with the study published in DNA Research (Kravatsky et al., 2015. Our data indicate that H3K4me3 marks often coincide with hot spots of DSBs in HEK293T cells and that the mapping of these hot spots is important for cancer genomic studies.

  12. Postharvest Propionic Acid and Hot Water Treatment for the Control of Black Spot ntrol of Black Spot

    Directory of Open Access Journals (Sweden)

    Tao Liu

    2014-02-01

    Full Text Available Black spot disease caused by Alternaria alternata (Fr. is one of the main postharvest diseases of Chinese Ya pears and causes serious economic loss during storage. The fungi-toxicity of postharvest propionic acid and hot water treatment at different concentrations and temperatures was investigated in vitro and directly on Ya pears. The in vitro results showed that A. alternata was susceptible to propionic acid solutions at all tested temperatures. The toxicity of propionic acid increased linearly and the LC 99 decreased from 13.43 to 4.31 g/L with an increase in temperature from 20 to 45C. The results in Ya pears showed that propionic acid and hot water treatment dramatically controlled black spot disease and 99% inhibition was achieved upon treatment with 9 g/L propionic acid solution for 30 min at 45C. Furthermore, the treatment inhibited the respiration of fruits and did not cause any adverse effects on other quality parameters; however, slight surface injury was noted at 9 g/L. The safety of propionic acid treatment was further confirmed by residue analysis. The results of this study suggest that postharvest propionic acid and hot water treatment is an alternative method for the control of black spot disease in Ya pears.

  13. Wavelength modulated SERS hot spot distribution in 1D nanostructures on metal film

    Science.gov (United States)

    Wang, Lili; Zeng, Xiping; Liu, Ting; Zhang, Xuemei; Wei, Hua; Huang, Yingzhou; Liu, Anping; Wang, Shuxia; Wen, Weijia

    2016-10-01

    Surface plasmons confining strong electromagnetic fields near metal surfaces, well-known as hot spots, provide an extremely efficient platform for surface-enhanced Raman scattering (SERS). In this work, SERS spectra of probing molecules in a silver particle-wire 1D nanostructure on a thin gold film are investigated. The Raman features of SERS spectra collected at the particle-wire joints exhibit an obvious wavelength dependence phenomenon. This result is confirmed electromagnetic field simulation, revealing that hot spot distribution is sensitively influenced by the wavelength of incident light at the joints. Further studies indicate this wavelength dependence of hot spot distribution is immune to influence from the geometric shape of the particle or the angle between wire and particle, which improves fabrication tolerance. This technology may have promising applications in surface plasmon related fields, such as ultrasensors, solar energy and selective surface catalysis.

  14. MICROSTRUCTURE AND PROPERTIES OF DEEP CROGENIC TREATMENT ELECTRODES FOR SPOT WELDING HOT DIP GALVANIZED STEEL

    Institute of Scientific and Technical Information of China (English)

    Wu Zhisheng; Liu Cuirong; Wang Jiuhai; Shan Ping; Hu Shengsun; Lian Jinrui

    2005-01-01

    The microstructure and elements distribution of the deep cryogenic treatment electrodes and non-cryogenic treatment electrodes for spot welding hot dip galvanized steel are observed by a scanning electrical microscope. The grain sizes, the resistivity and the hardness of the electrodes before and after deep cryogenic treatment are measured by X-ray diffraction, the DC double arms bridge and the Brinell hardness testing unit respectively. The spot welding process performance of hot dip galvanized steel plate is tested and the relationship between microstructure and physical properties of deep cryogenic treatment electrodes is analyzed. The experimental results show that deep cryogenic treatment makes Cr, Zr in deep cryogenic treatment electrodes emanate dispersedly and makes the grain of deep cryogenic treatment electrodes smaller than non-cryogenic treatment ones so that the electrical conductivity and the thermal conductivity of deep cryogenic treatment electrodes are improved very much, which make spot welding process performance of the hot dip galvanized steel be improved.

  15. Testing a class of non-Kerr metrics with hot spots orbiting SgrA*

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Dan; Li, Zilong; Bambi, Cosimo, E-mail: danliu12@fudan.edu.cn, E-mail: zilongli@fudan.edu.cn, E-mail: bambi@fudan.edu.cn [Center for Field Theory and Particle Physics and Department of Physics, Fudan University, 220 Handan Road, 200433 Shanghai (China)

    2015-01-01

    SgrA*, the supermassive black hole candidate at the Galactic Center, exhibits flares in the X-ray, NIR, and sub-mm bands that may be interpreted within a hot spot model. Light curves and images of hot spots orbiting a black hole are affected by a number of special and general relativistic effects, and they can be potentially used to check whether the object is a Kerr black hole of general relativity. However, in a previous study we have shown that the relativistic features are usually subdominant with respect to the background noise and the model-dependent properties of the hot spot, and eventually it is at most possible to estimate the frequency of the innermost stable circular orbit. In this case, tests of the Kerr metric are only possible in combination with other measurements. In the present work, we consider a class of non-Kerr spacetimes in which the hot spot orbit may be outside the equatorial plane. These metrics are difficult to constrain from the study of accretion disks and indeed current X-ray observations of stellar-mass and supermassive black hole candidates cannot put interesting bounds. Here we show that near future observations of SgrA* may do it. If the hot spot is sufficiently close to the massive object, the image affected by Doppler blueshift is brighter than the other one and this provides a specific observational signature in the hot spot's centroid track. We conclude that accurate astrometric observations of SgrA* with an instrument like GRAVITY should be able to test this class of metrics, except in the more unlikely case of a small viewing angle.

  16. Automated analysis of hot spot X-ray images at the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Khan, S. F., E-mail: khan9@llnl.gov; Izumi, N.; Glenn, S.; Tommasini, R.; Benedetti, L. R.; Ma, T.; Pak, A.; Springer, P.; Bradley, D. K.; Town, R. P. J. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Kyrala, G. A. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)

    2016-11-15

    At the National Ignition Facility, the symmetry of the hot spot of imploding capsules is diagnosed by imaging the emitted x-rays using gated cameras and image plates. The symmetry of an implosion is an important factor in the yield generated from the resulting fusion process. The x-ray images are analyzed by decomposing the image intensity contours into Fourier and Legendre modes. This paper focuses on the additional protocols for the time-integrated shape analysis from image plates. For implosions with temperatures above ∼4 keV, the hard x-ray background can be utilized to infer the temperature of the hot spot.

  17. Semi-automatic Epileptic Hot Spot Detection in ECD brain SPECT images

    Science.gov (United States)

    Papp, Laszlo; Zuhayra, Maaz; Henze, Eberhard

    A method is proposed to process ECD brain SPECT images representing epileptic hot spots inside the brain. For validation 35 ictal —interictal patient image data were processed. The images were registered by a normalized mutual information method, then the separation of the suspicious and normal brain areas were performed by two threshold-based segmentations. Normalization between the images was performed by local normal brain mean values. Based on the validation made by two medical physicians, minimal human intervention in the segmentation parameters was necessary to detect all epileptic spots and minimize the number of false spots inside the brain.

  18. Semi-Supervised Transductive Hot Spot Predictor Working on Multiple Assumptions

    KAUST Repository

    Wang, Jim Jing-Yan

    2014-05-23

    Protein-protein interactions are critically dependent on just a few residues (“hot spots”) at the interfaces. Hot spots make a dominant contribution to the binding free energy and if mutated they can disrupt the interaction. As mutagenesis studies require significant experimental efforts, there exists a need for accurate and reliable computational hot spot prediction methods. Compared to the supervised hot spot prediction algorithms, the semi-supervised prediction methods can take into consideration both the labeled and unlabeled residues in the dataset during the prediction procedure. The transductive support vector machine has been utilized for this task and demonstrated a better prediction performance. To the best of our knowledge, however, none of the transductive semi-supervised algorithms takes all the three semisupervised assumptions, i.e., smoothness, cluster and manifold assumptions, together into account during learning. In this paper, we propose a novel semi-supervised method for hot spot residue prediction, by considering all the three semisupervised assumptions using nonlinear models. Our algorithm, IterPropMCS, works in an iterative manner. In each iteration, the algorithm first propagates the labels of the labeled residues to the unlabeled ones, along the shortest path between them on a graph, assuming that they lie on a nonlinear manifold. Then it selects the most confident residues as the labeled ones for the next iteration, according to the cluster and smoothness criteria, which is implemented by a nonlinear density estimator. Experiments on a benchmark dataset, using protein structure-based features, demonstrate that our approach is effective in predicting hot spots and compares favorably to other available methods. The results also show that our method outperforms the state-of-the-art transductive learning methods.

  19. Identification of hot spots of malaria transmission for targeted malaria control.

    NARCIS (Netherlands)

    Bousema, T.; Drakeley, C.; Gesase, S.; Hashim, R.; Magesa, S.; Mosha, F.; Otieno, S.; Carneiro, I.; Cox, J.; Msuya, E.; Kleinschmidt, I.; Maxwell, C.; Greenwood, B.; Riley, E.; Sauerwein, R.W.; Chandramohan, D.; Gosling, R.

    2010-01-01

    BACKGROUND: Variation in the risk of malaria within populations is a frequently described but poorly understood phenomenon. This heterogeneity creates opportunities for targeted interventions but only if hot spots of malaria transmission can be easily identified. METHODS: We determined spatial patte

  20. QPO emission from moving hot spots on the surface of neutron stars: a model

    CERN Document Server

    Bachetti, Matteo; Kulkarni, Akshay; Burderi, Luciano; di Salvo, Tiziana

    2009-01-01

    We present recent results of 3D magnetohydrodynamic simulations of neutron stars with small misalignment angles, as regards the features in light curves produced by regular movements of the hot spots during accretion onto the star. In particular, we show that the variation of position of the hot spot created by the infalling matter, as observed in 3D simulations, can produce high frequency Quasi Periodic Oscillations with frequencies associated with the inner zone of the disk. Simulations show that the usual assumption of a fixed hot spot near the polar region is valid only for misalignment angles relatively large. Otherwise, two phenomena challenge the assumption: one is the presence of Rayleigh-Taylor instabilities at the disk-magnetospheric boundary (e.g. Kulkarni & Romanova 2008), which produce tongues of accreting matter that can reach the star almost anywhere between the equator and the polar region; the other one is the motion of the hot spot around the magnetic pole during stable accretion (e.g. R...

  1. CFD analysis of hot spot formation through a fixed bed reactor of Fischer-Tropsch synthesis

    Directory of Open Access Journals (Sweden)

    Hamed Aligolzadeh

    2015-12-01

    Full Text Available One of the interesting methods for conversion of synthesis gas to heavy hydrocarbons is Fischer–Tropsch process. The process has some bottlenecks, such as hot spot formation and low degree of conversion. In this work, computational fluid dynamics technique was used to simulate conversion of synthetic gas and product distribution. Also, hot spot formation in the catalytic fixed-bed reactor was investigated in several runs. Simulation results indicated that hot spot formation occurred more likely in the early and middle part of reactor due to high reaction rates. Based on the simulation results, the temperature of hot spots increased with increase in the inlet temperature as well as pressure. Among the many CFD runs conducted, it is found that the optimal temperature and pressure for Fischer–Tropsch synthesis are 565 K and 20 bar, respectively. As it seems that the reactor shall work very well under optimal conditions, the reaction rates and catalyst duration would simultaneously be maximum .

  2. Narrow-bandwidth high-order harmonics driven by long-duration hot spots

    Science.gov (United States)

    Kozlov, Maxim; Kfir, Ofer; Fleischer, Avner; Kaplan, Alex; Carmon, Tal; Schwefel, Harald G. L.; Bartal, Guy; Cohen, Oren

    2012-06-01

    We predict and investigate the emission of high-order harmonics by atoms that cross intense laser hot spots that last for a nanosecond or longer. An atom that moves through a nanometer-scale hot spot at characteristic thermal velocity can emit high-order harmonics in a similar fashion to an atom that is irradiated by a short-duration (picosecond-scale) laser pulse. We analyze the collective emission from a thermal gas and from a jet of atoms. In both cases, the line shape of a high-order harmonic exhibits a narrow spike with spectral width that is determined by the bandwidth of the driving laser. Finally, we discuss a scheme for producing long-duration laser hot spots with intensity in the range of the intensity threshold for high-harmonic generation. In the proposed scheme, the hot spot is produced by a long laser pulse that is consecutively coupled to a high-quality micro-resonator and a metallic nano-antenna. This system may be used for generating ultra-narrow bandwidth extreme-ultraviolet radiation through frequency up-conversion of a low-cost compact pump laser.

  3. Studies of plasmonic hot-spot translation by a metal-dielectric layered superlens

    DEFF Research Database (Denmark)

    Thoreson, Mark D.; Nielsen, Rasmus Bundgaard; West, Paul R.;

    2011-01-01

    We have studied the ability of a lamellar near-field superlens to transfer an enhanced electromagnetic field to the far side of the lens. In this work, we have experimentally and numerically investigated superlensing in the visible range. By using the resonant hot-spot field enhancements from opt...

  4. Piezo-Electric Hypothesis for Hot Spot Formation Leading to Detonation

    Science.gov (United States)

    Montgomery, D. S.; Cawkwell, M. J.; Ramos, K. J.

    2015-06-01

    The impact to detonation sequence has been a long standing mystery in high explosives (HE). It is widely recognized that detonation begins in spatially-localized ``hot spots'' where chemistry initiates, but the physical mechanisms leading to hot spot formation are unknown. Here we revisit an old hypothesis, first suggested by Maycock and Grabenstein, that piezo-electric effects may be the cause of hot spot formation since most solid HE materials are observed to be highly piezo-electric. In this scenario, shock-induced pressure leads to electric fields of 100's MV/m, sufficient for dielectric breakdown and breaking chemical bonds, rather than via thermal effects. Extrapolation of statically measured piezo-electric coefficients for several HE materials suggests that shock pressures > 100-kbar might lead to field strengths > 100 - 1000 MV/m, but no definitive experimental proof has been obtained to support this. Here we discuss possible experiments to test this hypothesis by measuring the electric field in dynamic HE experiments correlated with hot spot formation. Work performed under the auspices of DOE by LANL under contract DE-AC52-06NA25396.

  5. Non-equilibrium between ions and electrons inside hot spots from National Ignition Facility experiments

    Directory of Open Access Journals (Sweden)

    Zhengfeng Fan

    2017-01-01

    Full Text Available The non-equilibrium between ions and electrons in the hot spot can relax the ignition conditions in inertial confinement fusion [Fan et al., Phys. Plasmas 23, 010703 (2016], and obvious ion-electron non-equilibrium could be observed by our simulations of high-foot implosions when the ion-electron relaxation is enlarged by a factor of 2. On the other hand, in many shots of high-foot implosions on the National Ignition Facility, the observed X-ray enhancement factors due to ablator mixing into the hot spot are less than unity assuming electrons and ions have the same temperature [Meezan et al., Phys. Plasmas 22, 062703 (2015], which is not self-consistent because it can lead to negative ablator mixing into the hot spot. Actually, this non-consistency implies ion-electron non-equilibrium within the hot spot. From our study, we can infer that ion-electron non-equilibrium exists in high-foot implosions and the ion temperature could be ∼9% larger than the equilibrium temperature in some NIF shots.

  6. Hot Spots and Trends in Developing China's Tourism Industry

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    It is stated in China's Tourism Development from 2002 to 2004: Analysis and Prediction, written by experts organized by Research Center of Tourism of China Academy of Social Science that eight hot spots have arisen and future trends in Chinese tourism development has been embodied in eight aspects since 2002.

  7. THE PROBLEM OF HOT-SPOTS IN MICROWAVE EQUIPMENT USED FOR PREPARATORY TECHNIQUES - THEORY AND PRACTICE

    NARCIS (Netherlands)

    KOK, LP; BOON, ME; SMID, HM

    1993-01-01

    Electron microscopists who wants to use a microwave (MW) oven to stimulate preparatory processes are sooner or later confronted with the problem of hot spots. It soon becomes clear to the user of any MW oven that the energy distribution-thus the speed of absorbing energy, and hence warming up-varies

  8. THE PROBLEM OF HOT-SPOTS IN MICROWAVE EQUIPMENT USED FOR PREPARATORY TECHNIQUES - THEORY AND PRACTICE

    NARCIS (Netherlands)

    KOK, LP; BOON, ME; SMID, HM

    1993-01-01

    Electron microscopists who wants to use a microwave (MW) oven to stimulate preparatory processes are sooner or later confronted with the problem of hot spots. It soon becomes clear to the user of any MW oven that the energy distribution-thus the speed of absorbing energy, and hence warming up-varies

  9. A Machine Learning Approach for Hot-Spot Detection at Protein-Protein Interfaces

    NARCIS (Netherlands)

    Melo, Rita; Fieldhouse, Robert; Melo, André; Correia, João D G; Cordeiro, Maria Natália D S; Gümüş, Zeynep H; Costa, Joaquim; Bonvin, Alexandre M J J|info:eu-repo/dai/nl/113691238; de Sousa Moreira, Irina|info:eu-repo/dai/nl/412025000

    2016-01-01

    Understanding protein-protein interactions is a key challenge in biochemistry. In this work, we describe a more accurate methodology to predict Hot-Spots (HS) in protein-protein interfaces from their native complex structure compared to previous published Machine Learning (ML) techniques. Our model

  10. Hot-spot application of biocontrol agents to replace pesticides in large scale commercial rose farms in Kenya

    DEFF Research Database (Denmark)

    Gacheri, Catherine; Kigen, Thomas; Sigsgaard, Lene

    2015-01-01

    Rose (Rosa hybrida L.) is the most important ornamental crop in Kenya, with huge investments in pest management. We provide the first full-scale, replicated experiment comparing cost and yield of conventional two-spotted spider mite (Tetranychus urticae Koch) control with hot-spot applications of....... persimilis hot-spot treatments in commercial cut rose production, effectively reducing pest management costs with no loss in crop yield.......Rose (Rosa hybrida L.) is the most important ornamental crop in Kenya, with huge investments in pest management. We provide the first full-scale, replicated experiment comparing cost and yield of conventional two-spotted spider mite (Tetranychus urticae Koch) control with hot-spot applications...... of the predatory mite Phytoseiulus persimilis (Acari: Phytoseidae) in large commercial rose greenhouses. Hot-spot treatments replaced acaricides except at high infestations and the two treatments were applied in seven greenhouses each. With the conventional treatment, acaricides were applied when T. urticae...

  11. Galapagos-OIB signature in southern Central America: Mantle refertilization by arc-hot spot interaction

    Science.gov (United States)

    Gazel, Esteban; Carr, Michael J.; Hoernle, Kaj; Feigenson, Mark D.; Szymanski, David; Hauff, Folkmar; van den Bogaard, Paul

    2009-02-01

    Although most Central American magmas have a typical arc geochemical signature, magmas in southern Central America (central Costa Rica and Panama) have isotopic and trace element compositions with an ocean island basalt (OIB) affinity, similar to the Galapagos-OIB lavas (e.g., Ba/La 10, 206Pb/204Pb > 18.8). Our new data for Costa Rica suggest that this signature, unusual for a convergent margin, has a relatively recent origin (Late Miocene ˜6 Ma). We also show that there was a transition from typical arc magmas (analogous to the modern Nicaraguan volcanic front) to OIB-like magmas similar to the Galapagos hot spot. The geographic distribution of the Galapagos signature in recent lavas from southern Central America is present landward from the subduction of the Galapagos hot spot tracks (the Seamount Province and the Cocos/Coiba Ridge) at the Middle American Trench. The higher Pb isotopic ratios, relatively lower Sr and Nd isotopic ratios, and enriched incompatible-element signature of central Costa Rican magmas can be explained by arc-hot spot interaction. The isotopic ratios of central Costa Rican lavas require the subducting Seamount Province (Northern Galapagos Domain) component, whereas the isotopic ratios of the adakites and alkaline basalts from southern Costa Rica and Panama are in the geochemical range of the subducting Cocos/Coiba Ridge (Central Galapagos Domain). Geological and geochemical evidence collectively indicate that the relatively recent Galapagos-OIB signature in southern Central America represents a geochemical signal from subducting Galapagos hot spot tracks, which started to collide with the margin ˜8 Ma ago. The Galapagos hot spot contribution decreases systematically along the volcanic front from central Costa Rica to NW Nicaragua.

  12. Hot-spot application of biocontrol agents to replace pesticides in large scale commercial rose farms in Kenya

    DEFF Research Database (Denmark)

    Gacheri, Catherine; Kigen, Thomas; Sigsgaard, Lene

    2015-01-01

    Rose (Rosa hybrida L.) is the most important ornamental crop in Kenya, with huge investments in pest management. We provide the first full-scale, replicated experiment comparing cost and yield of conventional two-spotted spider mite (Tetranychus urticae Koch) control with hot-spot applications....... persimilis hot-spot treatments in commercial cut rose production, effectively reducing pest management costs with no loss in crop yield....

  13. Cassini VIMS Spectra of the Thermal Emission from Hot Spots Along Enceladus South Pole Fissures

    Science.gov (United States)

    Goguen, Jay D.; Buratti, Bonnie J.; Cassini VIMS Team

    2016-10-01

    Most of the south pole fissure region has not been directly illuminated by sunlight since the sub-solar point moved into the northern hemisphere in 2009, thereby eliminating the background of reflected sunlight at VIMS wavelengths and making the fissure thermal emission readily measureable. Since then, VIMS has measured spectra of at least 11 hot spots along the fissures. Most of these measurements were acquired in ride-along mode with CIRS as the prime instrument. During at least 2 encounters, VIMS and CIRS acquired simultaneous or near-simultaneous spectra of the same fissure location. VIMS spectra include multiple hot spots along Damascus, Baghdad, Cairo, and a likely hot spot on Alexandria.All of the VIMS spectra examined to date are consistent with this scenario of a self-regulating fissure maximum T~200 K with brighter VIMS emissions corresponding to fissures up to ~20 m wide. Emission from the warm fissure interior walls dominate the VIMS spectra with <15% contributed by conductive heating of the adjacent terrain at VIMS wavelengths.CIRS spectra report slightly cooler T's due to CIRS increased sensitivity to lower T emission at longer wavelengths and averaging over contributions from both the hottest and cooler areas. Combined analysis of the CIRS and VIMS spectra spanning 3 to 500 micron wavelengths promises to reveal the distribution of [T, area] near the fissures that cannot be spatially resolved. This [T, area] distribution holds the key to understanding how heat is transferred to the surface within a few 100 m of the fissures.The VIMS-detected emission is concentrated in localized hot spots along the fissures and does not seem to be distributed continuously along them. CIRS spectra suggest a more continuous distribution of the emission along the fissure length. Jets locations also are distributed along the fissure length and it appears that the VIMS-detected hot spots in general correlate with jet locations, but not all of the jet locations have been

  14. ELIPGRID-PC: A PC program for calculating hot spot probabilities

    Energy Technology Data Exchange (ETDEWEB)

    Davidson, J.R.

    1994-10-01

    ELIPGRID-PC, a new personal computer program has been developed to provide easy access to Singer`s 1972 ELIPGRID algorithm for hot-spot detection probabilities. Three features of the program are the ability to determine: (1) the grid size required for specified conditions, (2) the smallest hot spot that can be sampled with a given probability, and (3) the approximate grid size resulting from specified conditions and sampling cost. ELIPGRID-PC also provides probability of hit versus cost data for graphing with spread-sheets or graphics software. The program has been successfully tested using Singer`s published ELIPGRID results. An apparent error in the original ELIPGRID code has been uncovered and an appropriate modification incorporated into the new program.

  15. Rapid experimental SAD phasing and hot-spot identification with halogenated fragments

    Directory of Open Access Journals (Sweden)

    Joseph D. Bauman

    2016-01-01

    Full Text Available Through X-ray crystallographic fragment screening, 4-bromopyrazole was discovered to be a `magic bullet' that is capable of binding at many of the ligand `hot spots' found in HIV-1 reverse transcriptase (RT. The binding locations can be in pockets that are `hidden' in the unliganded crystal form, allowing rapid identification of these sites for in silico screening. In addition to hot-spot identification, this ubiquitous yet specific binding provides an avenue for X-ray crystallographic phase determination, which can be a significant bottleneck in the determination of the structures of novel proteins. The anomalous signal from 4-bromopyrazole or 4-iodopyrazole was sufficient to determine the structures of three proteins (HIV-1 RT, influenza A endonuclease and proteinase K by single-wavelength anomalous dispersion (SAD from single crystals. Both compounds are inexpensive, readily available, safe and very soluble in DMSO or water, allowing efficient soaking into crystals.

  16. Exploring User Acceptance Of Free Wireless Fidelity Public Hot Spots: An Emperical Study

    Directory of Open Access Journals (Sweden)

    Ezejiofo Patrick Udeh

    2008-01-01

    Full Text Available Research regarding commercial and free wireless fidelity (Wi-Fi public hot spots acceptance and adoption is sketchy. Therefore, it has become imperative to understand the critical factors that affect their acceptance. The focus of this study is free Wi-Fi public hot spot users, with the objective to better understand their user acceptance. In doing so, this study integrated two well-established initial acceptance models, specifically, the technology acceptance model and the diffusion of innovation theory. This study was conducted using an on-line survey that collected data from 129 users. It uses the Partial Least Square (PLS technique to examine the relationship between variables. The results indicate that each critical factor has direct or indirect positive effects on current use and/or future use intention, which confirmed the majority of the proposed hypotheses. Relative advantage emerged as the only construct with a direct positive effect on both current use and future use intentions.

  17. STUDY ON LOCATION OF HOT SPOT AT TUBE WALL FOR FIRED CYLINDRICAL FURNACE COMBUSTION

    Institute of Scientific and Technical Information of China (English)

    Wang Taiyong; Lu Shizhong; Xü Zhixue

    2004-01-01

    Based on the analysis of heat radiation intensity from flame,a new mathematical model of the tube-wall temperature of heated tubes is devel-oped by taking down-fired,upright-tube cylindrical furnace for example.The proposed mathematical model can be employed to indicate both the position and size of the hot spot at fire-facing wall of heated tube of combustion chamber,and is characteristic of sim-plicity and efficiency.If coupled with thermoelectric couple or infrared viewer,the presented location method of com-bustion hot spot can offer engineers very valuable proposal to keep furnace running more safely.The same is true for any other type of tubular furnaces.

  18. Influence of hot spot features on the initiation characteristics of heterogeneous nitromethane

    Energy Technology Data Exchange (ETDEWEB)

    Dattelbaum, Dana M [Los Alamos National Laboratory; Sheffield, Stephen A [Los Alamos National Laboratory; Stahl, David B [Los Alamos National Laboratory; Dattelbaum, Andrew M [Los Alamos National Laboratory; Engelke, Ray [Los Alamos National Laboratory

    2010-01-01

    To gain insights into the critical hot spot features influencing energetic materials initiation characteristics, well-defined micron-scale particles have been intentionally introduced into the homogeneous explosive nitromethane (NM). Two types of potential hot spot origins have been examined - shock impedance mismatches using solid silica beads, and porosity using hollow microballoons - as well as their sizes and inter-particle separations. Here, we present the results of several series of gas gun-driven plate impact experiments on NM/particle mixtures with well-controlled shock inputs. Detailed insights into the nature of the reactive flow during the build-up to detonation have been obtained from the response of in-situ electromagnetic gauges, and the data have been used to establish Pop-plots (run-distance-to-detonation vs. shock input pressure) for the mixtures. Comparisons of sensitization effects and energy release characteristics relative to the initial shock front between the solid and hollow beads are presented.

  19. Characterization of hot spots in microstructured reactors for fast and exothermic reactions in mixing regime

    OpenAIRE

    Haber, Julien; Kashid, Madhavanand N.; Borhani, Navid; Jiang, Bo; Maeder, Thomas; Thome, John Richard; Renken, Albert; Kiwi-Minsker, Lioubov

    2012-01-01

    The intensification of fast exothermic reactions can be achieved by using microstructured reactors (MSR) which provide improved mass & heat transfer rates leading to higher overall reaction kinetics. But for highly exothermic reactions the heat evacuation becomes not efficient enough and unwanted hot spots are formed. In this study, first the mixing in MSR is quantified for different geometries and then temperature profiles are measured using a novel quantitative IR-thermometry method. The re...

  20. Analysis of Binding Site Hot Spots on the Surface of Ras GTPase

    Energy Technology Data Exchange (ETDEWEB)

    Buhrman, Greg; O; #8242; Connor, Casey; Zerbe, Brandon; Kearney, Bradley M.; Napoleon, Raeanne; Kovrigina, Elizaveta A.; Vajda, Sandor; Kozakov, Dima; Kovrigin, Evgenii L.; Mattos, Carla (NCSU); (MCW); (BU)

    2012-09-17

    We have recently discovered an allosteric switch in Ras, bringing an additional level of complexity to this GTPase whose mutants are involved in nearly 30% of cancers. Upon activation of the allosteric switch, there is a shift in helix 3/loop 7 associated with a disorder to order transition in the active site. Here, we use a combination of multiple solvent crystal structures and computational solvent mapping (FTMap) to determine binding site hot spots in the 'off' and 'on' allosteric states of the GTP-bound form of H-Ras. Thirteen sites are revealed, expanding possible target sites for ligand binding well beyond the active site. Comparison of FTMaps for the H and K isoforms reveals essentially identical hot spots. Furthermore, using NMR measurements of spin relaxation, we determined that K-Ras exhibits global conformational dynamics very similar to those we previously reported for H-Ras. We thus hypothesize that the global conformational rearrangement serves as a mechanism for allosteric coupling between the effector interface and remote hot spots in all Ras isoforms. At least with respect to the binding sites involving the G domain, H-Ras is an excellent model for K-Ras and probably N-Ras as well. Ras has so far been elusive as a target for drug design. The present work identifies various unexplored hot spots throughout the entire surface of Ras, extending the focus from the disordered active site to well-ordered locations that should be easier to target.

  1. Hot spot analysis for driving the development of hits into leads in fragment based drug discovery

    OpenAIRE

    Hall, David R.; Ngan, Chi Ho; Zerbe, Brandon S.; Kozakov, Dima; Vajda, Sandor

    2011-01-01

    Fragment based drug design (FBDD) starts with finding fragment-sized compounds that are highly ligand efficient and can serve as a core moiety for developing high affinity leads. Although the core-bound structure of a protein facilitates the construction of leads, effective design is far from straightforward. We show that protein mapping, a computational method developed to find binding hot spots and implemented as the FTMap server, provides information that complements the fragment screening...

  2. Hot Spot Electron Temperature from X-Ray Continuum Measurements on the NIF

    Science.gov (United States)

    Jarrott, Leonard; Chen, Hui; Izumi, Nobuhiko; Khan, Shahab; Ma, Tammy; Nagel, Sabrina; Pak, Arthur; Patel, Pravesh; Schneider, Marilyn; Scott, Howard

    2015-11-01

    We report on direct measurements of the electron temperature within the hot spot of inertially confined, layered, spherical implosions on the National Ignition Facility using a new differential filtering diagnostic. Measurements of the DT and DD ion temperatures using neutron time-of-flight detectors are complicated by the contribution of hot spot motion to the peak width, which may produce an apparent temperature higher than the thermal temperature. The electron temperature is not sensitive to this non-thermal velocity and is thus a valuable input to interpreting the stagnated hot spot conditions. Here we discuss a new electron temperature measurement using the high energy (>15 keV) emitted continuum from the hotspot that can escape with minimal attenuation from the compressed fuel/shell. We will discuss the physics considerations for design of this new large-pinhole, hard x-ray imaging technique, and show preliminary data acquired from symcaps and DT-layered implosions. Validation of this technique against simulations and other diagnostics is performed to estimate the accuracy of the measurement. This work performed under the auspices of U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

  3. Resolving hot spot microstructure using x-ray penumbral imaging (invited)

    Energy Technology Data Exchange (ETDEWEB)

    Bachmann, B., E-mail: bachmann2@llnl.gov; Field, J.; Masters, N.; Pardini, T.; Rygg, J. R.; Benedetti, L. R.; Döppner, T.; Izumi, N.; LePape, S.; Ma, T.; MacPhee, A. G.; Nagel, S.; Patel, P.; Spears, B.; Landen, O. L. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Hilsabeck, T.; Reed, C.; Alexander, N.; Forsman, A. [General Atomics, San Diego, California 92186 (United States)

    2016-11-15

    We have developed and fielded x-ray penumbral imaging on the National Ignition Facility in order to enable sub-10 μm resolution imaging of stagnated plasma cores (hot spots) of spherically shock compressed spheres and shell implosion targets. By utilizing circular tungsten and tantalum apertures with diameters ranging from 20 μm to 2 mm, in combination with image plate and gated x-ray detectors as well as imaging magnifications ranging from 4 to 64, we have demonstrated high-resolution imaging of hot spot plasmas at x-ray energies above 5 keV. Here we give an overview of the experimental design criteria involved and demonstrate the most relevant influences on the reconstruction of x-ray penumbral images, as well as mitigation strategies of image degrading effects like over-exposed pixels, artifacts, and photon limited source emission. We describe experimental results showing the advantages of x-ray penumbral imaging over conventional Fraunhofer and photon limited pinhole imaging and showcase how internal hot spot microstructures can be resolved.

  4. The Uses and Impacts of Mobile Computing Technology in Hot Spots Policing.

    Science.gov (United States)

    Koper, Christopher S; Lum, Cynthia; Hibdon, Julie

    2015-12-01

    Recent technological advances have much potential for improving police performance, but there has been little research testing whether they have made police more effective in reducing crime. To study the uses and crime control impacts of mobile computing technology in the context of geographically focused "hot spots" patrols. An experiment was conducted using 18 crime hot spots in a suburban jurisdiction. Nine of these locations were randomly selected to receive additional patrols over 11 weeks. Researchers studied officers' use of mobile information technology (IT) during the patrols using activity logs and interviews. Nonrandomized subgroup and multivariate analyses were employed to determine if and how the effects of the patrols varied based on these patterns. Officers used mobile computing technology primarily for surveillance and enforcement (e.g., checking automobile license plates and running checks on people during traffic stops and field interviews), and they noted both advantages and disadvantages to its use. Officers did not often use technology for strategic problem-solving and crime prevention. Given sufficient (but modest) dosages, the extra patrols reduced crime at the hot spots, but this effect was smaller in places where officers made greater use of technology. Basic applications of mobile computing may have little if any direct, measurable impact on officers' ability to reduce crime in the field. Greater training and emphasis on strategic uses of IT for problem-solving and crime prevention, and greater attention to its behavioral effects on officers, might enhance its application for crime reduction. © The Author(s) 2016.

  5. Predictions of hot spot residues at protein-protein interfaces using support vector machines.

    Directory of Open Access Journals (Sweden)

    Stefano Lise

    Full Text Available Protein-protein interactions are critically dependent on just a few 'hot spot' residues at the interface. Hot spots make a dominant contribution to the free energy of binding and they can disrupt the interaction if mutated to alanine. Here, we present HSPred, a support vector machine(SVM-based method to predict hot spot residues, given the structure of a complex. HSPred represents an improvement over a previously described approach (Lise et al, BMC Bioinformatics 2009, 10:365. It achieves higher accuracy by treating separately predictions involving either an arginine or a glutamic acid residue. These are the amino acid types on which the original model did not perform well. We have therefore developed two additional SVM classifiers, specifically optimised for these cases. HSPred reaches an overall precision and recall respectively of 61% and 69%, which roughly corresponds to a 10% improvement. An implementation of the described method is available as a web server at http://bioinf.cs.ucl.ac.uk/hspred. It is free to non-commercial users.

  6. Resolving hot spot microstructure using x-ray penumbral imaging (invited)

    Science.gov (United States)

    Bachmann, B.; Hilsabeck, T.; Field, J.; Masters, N.; Reed, C.; Pardini, T.; Rygg, J. R.; Alexander, N.; Benedetti, L. R.; Döppner, T.; Forsman, A.; Izumi, N.; LePape, S.; Ma, T.; MacPhee, A. G.; Nagel, S.; Patel, P.; Spears, B.; Landen, O. L.

    2016-11-01

    We have developed and fielded x-ray penumbral imaging on the National Ignition Facility in order to enable sub-10 μm resolution imaging of stagnated plasma cores (hot spots) of spherically shock compressed spheres and shell implosion targets. By utilizing circular tungsten and tantalum apertures with diameters ranging from 20 μm to 2 mm, in combination with image plate and gated x-ray detectors as well as imaging magnifications ranging from 4 to 64, we have demonstrated high-resolution imaging of hot spot plasmas at x-ray energies above 5 keV. Here we give an overview of the experimental design criteria involved and demonstrate the most relevant influences on the reconstruction of x-ray penumbral images, as well as mitigation strategies of image degrading effects like over-exposed pixels, artifacts, and photon limited source emission. We describe experimental results showing the advantages of x-ray penumbral imaging over conventional Fraunhofer and photon limited pinhole imaging and showcase how internal hot spot microstructures can be resolved.

  7. Magnetotransport of multiple-band nearly antiferromagnetic metals due to hot-spot scattering

    Science.gov (United States)

    Koshelev, A. E.

    2016-09-01

    Multiple-band electronic structure and proximity to antiferromagnetic (AF) instability are the key properties of iron-based superconductors. We explore the influence of scattering by the AF spin fluctuations on transport of multiple-band metals above the magnetic transition. A salient feature of scattering on the AF fluctuations is that it is strongly enhanced at the Fermi surface locations where the nesting is perfect ("hot spots" or "hot lines"). We review derivation of the collision integral for the Boltzmann equation due to AF-fluctuations scattering. In the paramagnetic state, the enhanced scattering rate near the hot lines leads to anomalous behavior of electronic transport in magnetic field. We explore this behavior by analytically solving the Boltzmann transport equation with approximate transition rates. This approach accounts for return scattering events and is more accurate than the relaxation-time approximation. The magnetic-field dependences are characterized by two very different field scales: the lower scale is set by the hot-spot width and the higher scale is set by the total scattering amplitude. A conventional magnetotransport behavior is limited to magnetic fields below the lower scale. In the wide range in-between these two scales, the longitudinal conductivity has linear dependence on the magnetic field and the Hall conductivity has quadratic dependence. The linear dependence of the diagonal component reflects growth of the Fermi-surface area affected by the hot spots proportional to the magnetic field. We discuss applicability of this theoretical framework for describing of anomalous magnetotransport properties in different iron pnictides and chalcogenides in the paramagnetic state.

  8. Hot Spots and Not Spots: Addressing Infrastructure and Service Provision through Combined Approaches in Rural Scotland

    Directory of Open Access Journals (Sweden)

    Sarah Skerratt

    2010-06-01

    Full Text Available There is widespread acceptance that the absence or presence of infrastructure and services in rural areas can lead to cycles of decline or resilience in these localities. It is also accepted that in remoter areas, population sparsity leads to a higher unit cost for delivery of services and infrastructure, and that private sector providers do not find such areas attractive for investment. At the same time, there is a reduction in spending capability within the public sector due to the significant impact of economic crisis on their resource base, affecting provision of services. How are these seemingly intractable challenges being addressed? Using an interpretive policy analysis approach [1] and narrative tools, the storyline of policy statements, approaches and policies in Scotland is presented and discussed, within a wider European setting. This is complemented by a brief presentation of public-private and third sector initiatives in response to service and infrastructure challenges in rural Scotland. The paper concludes with the argument that we are facing two alternatives—the current “hot spots” and “not spots” pattern of provision, where the fittest survive, or further shifts towards strategic, cross-sectoral investment which gives scope for more cohesive development for rural communities.

  9. Wood ant nests as hot spots of carbon dioxide production and cold spots of methane oxidation in temperate forests

    Science.gov (United States)

    Jilkova, Veronika; Picek, Tomas; Cajthaml, Tomas; Frouz, Jan

    2016-04-01

    Wood ant nests are known as hot spots of carbon dioxide (CO2) production and are also thought to affect methane (CH4) flux. Stable high temperatures are maintained in ant nests even in cold environments. Here we focused on quantification of CO2 and CH4 flux in wood ant nests, contribution of ants and microbes to CO2 production, properties of nest material that affect CO2 production and the role of ants and microbes in the maintenance of nest temperature. The research was conducted in temperate and boreal forests inhabited by wood ants (Formica s. str.). Gas fluxes were measured either by an infrared gas analyser or a static chamber technique. Ants and nest materials were also incubated in a laboratory. Material properties potentially influencing CO2 flux, such as moisture, nutrient content or temperature were determined. According to the results, CH4 oxidation was lower in wood ant nests than in the surrounding forest soil suggesting that some characteristics of ant nests hinder CH4 oxidation or promote CH4 production. These characteristics were mainly available carbon and nitrogen contents. Wood ant nests clearly are hot spots of CO2 production in temperate forests originating mainly from ant and also from microbial metabolism. Most important properties positively affecting CO2 production were found to be moisture, nutrient content and temperature. Nest temperature is maintained by ant and microbial metabolism; nests from colder environments produce more metabolic heat to maintain similar temperature as nests from warmer environments. In conclusion, as the abundance of wood ant nests in some forests can be very high, ant nests may largely increase heterogeneity in greenhouse gas fluxes in forest ecosystems.

  10. Biological impacts of "hot-spot" mutations of hepatitis B virus X proteins are genotype B and C differentiated

    Institute of Scientific and Technical Information of China (English)

    Xu Lin; Xiao Xu; Qing-Ling Huang; Yu-Qing Liu; Da-Li Zheng; Wan-Nan Chen; Jian-Yin Lin

    2005-01-01

    AIM: To investigate the biological impacts of "hot-spot"mutations on genotype B and C HBV X proteins (HBx).METHODS: Five types of"hot-spot" mutations of genotype B or C HBV X genes, which sequentially lead to the amino acid substitutions of HBx as I127T, F132Y, K130M+V131I,I127T+K130M+V131I, or K130M+V131I+F132Y, respectively,were generated by means of site-directed mutagenesis.To evaluate the anti-proliferative effects, HBx or related mutants' expression vectors were transfected separately to the Chang cells by lipofectamine, and the cells were cultured in hygromycin selective medium for 14 d, drug-resistant colonies were fixed with cold methanol, stained with Giemsa dyes and scored (increase of the colonies indicated the reduction of the anti-proliferation activity,and vice versa). Different types of HBx expression vectors were co-transfected separately with the reporter plasmid pCMVβ to Chang cells, which were lysed 48 h post-transfection and the intra-cellular β-galactosidase activities were monitored (increase of the β-galactosidase activities indicated the reduction of the transactivation activity, and vice versa). All data obtained were calculated by paired-samples t-test.RESULTS: As compared to standard genotype B HBx,mutants of I127T and I127T+K130M+V131I showed higher transactivation and anti-proliferative activities, while the mutants of F132Y, K130M+V131I, and K130M+V131I+F132Y showed lower activities. As compared to standard genotype C HBx, I127T mutant showed higher transactivation activity, while the other four types of mutants showed no differences. With regard to anti-proliferative activity,compared to standard genotype C HBx, F132Y and K130M+V131I mutants showed lower activities, and K130M+V131I +F132Y mutant, on the other hand, showed higher activity,while the mutants of I127T and I127T+K130M+V131I showed no differences.CONCLUSION: "Hot-spot" mutations affect the antiproliferation and transactivation activities of genotype B and/or C HBx, and

  11. Radiation-induced chromosomal hot spots at G 1 and G 2 stages of human lymphocytes in culture

    Directory of Open Access Journals (Sweden)

    Murugesan R

    2003-01-01

    Full Text Available Radiation-induced chromosomal break points in cultured lymphocytes of normal healthy individuals as well as of those with certain genetic disorders are reported to be localized at certain specific loci (hot spots. These reports are based on studies carried out in lymphocytes irradiated at G 1 stage. The present study examines whether the location of hot spots and the frequency seen in cells irradiated at G 1 are similar to those irradiated at G 2 stage of the cell cycle and also tests whether cells of patients exhibit hot spots on irradiation.The results showed that the radiation induced chromosomal break points to be similar in those irradiated are G 1 and G 2 stages of the cell cycle and also that cells of patients exhibited chromosomal hot spots.

  12. Visualization and detection of spatio-temporal hot-spot and cluster for dengue in Petaling district, Malaysia

    Science.gov (United States)

    Rizwan, M.; Dass, Sarat C.; Sagayan, Vijanth

    2016-11-01

    Hot-spot and cluster detection is a part of disease surveillance to find out which regions are effected most by the disease. Analysis of these clusters of disease used for longer periods of time can lead to future prediction of disease outbreaks. Dengue is one of the most important arboviral disease in Malaysia which needs proper surveillance and control strategies. Our current study presents hot-spot and cluster detection of dengue outbreaks in the district of Petaling, Selangor in Malaysia during the year 2014 using the registered cases of dengue in the district. Detected hot-spot from this spatio-temporal analysis of registered dengue could provide a trend for the future dengue outbreak predictions. Use of dengue registered cases for the future prediction could be more effective then using land use variables and climate data. R software is used for the hot-spots detection. Custom clustering definition is considered for the analysis.

  13. Magma production rate along the Ninetyeast Ridge and its relationship to Indian plate motion and Kerguelen hot spot activity

    Digital Repository Service at National Institute of Oceanography (India)

    Sreejith, K.M.; Krishna, K.S.

    The Ninetyeast Ridge, a linear trace of the Kerguelen hot spot in the Indian Ocean, was emplaced on a rapidly drifting Indian plate. Magma production rates along the ridge track are computed using gravity-derived excess crustal thickness data...

  14. Turbulence and magnetic spots at the surface of hot massive stars

    CERN Document Server

    Cantiello, Matteo; Brandenburg, Axel; Del Sordo, Fabio; Käpylä, Petri; Langer, Norbert

    2010-01-01

    Hot luminous stars show a variety of phenomena in their photospheres and in their winds which still lack clear physical explanations at this time. Among these phenomena are non-thermal line broadening, line profile variability (LPVs), discrete absorption components (DACs), wind clumping and stochastically excited pulsations. Cantiello et al. (2009) argued that a convection zone close to the surface of hot, massive stars, could be responsible for some of these phenomena. This convective zone is caused by a peak in the opacity due to iron recombination and for this reason is referred as the "iron convection zone" (FeCZ). 3D MHD simulations are used to explore the possible effects of such subsurface convection on the surface properties of hot, massive stars. We argue that turbulence and localized magnetic spots at the surface are the likely consequence of subsurface convection in early type stars.

  15. Characterization of hot spots for natural chloroform formation: Relevance for groundwater quality

    Science.gov (United States)

    Jacobsen, Ole S.; Albers, Christian N.; Laier, Troels

    2015-04-01

    Chloroform soil hot spot may deteriorate groundwater quality and may even result in chloroform concentration exceeding the Danish maximum limit of 1 µg/L in groundwater for potable use. In order to characterize the soil properties important for the chloroform production, various ecosystems were examined with respect to soil air chloroform and soil organic matter type and content. Coniferous forest areas, responsible for highest chloroform concentrations, were examined on widely different scales from km to cm scale. Furthermore, regular soil gas measurements including chloroform were performed during 4 seasons at various depths, together with various meteorological measurements and soil temperature recordings. Laboratory incubation experiments were also performed on undisturbed soil samples in order to examine the role of various microbiota, fungi and bacteria. To identify hot spots responsible for the natural contamination we have measured the production of chloroform in the upper soil from different terrestrial systems. Field measurements of chloroform in top soil air were used as production indicators. The production was however not evenly distributed at any scale. The ecosystems seem to have quite different net-productions of chloroform from very low in grassland to very high in some coniferous forests. Within the forest ecosystem we found large variation in chloroform concentrations depending on vegetation. In beech forest we found the lowest values, somewhat higher in an open pine forest, but the highest concentrations were detected in spruce forest without any vegetation beneath. Within this ecotype, it appeared that the variation was also large; hot spots with 2-4 decades higher production than the surrounding area. These hot spots were not in any way visually different from the surroundings and were of variable size from 3 to 20 meters in diameter. Besides this, measurements within a seemingly homogenous hot spot showed that there was still high

  16. DNase I-hypersensitive sites and transcription factor-binding motifs within the mouse E beta meiotic recombination hot spot.

    Science.gov (United States)

    Shenkar, R; Shen, M H; Arnheim, N

    1991-04-01

    The second intron of the E beta gene in the mouse major histocompatibility complex is the site of a meiotic recombination hot spot. We detected two DNase I-hypersensitive sites in this intron in meiotic cells isolated from mouse testes. One site appears to be constitutive and is found in other tissues regardless of whether or not they express the E beta gene. Near this hypersensitive site are potential binding motifs for H2TF1/KBF1, NF kappa B, and octamer transcription factors. Gel retardation studies with mouse lymphoma cell nuclear extracts confirmed that each of these motifs is capable of binding protein. The binding of transcription factors may contribute to the enhancement of recombination potential by altering chromatin structure and increasing the accessibility of the DNA to the recombination machinery.

  17. Process window and defect monitoring using high-throughput e-beam inspection guided by computational hot spot detection

    Science.gov (United States)

    Wang, Fei; Zhang, Pengcheng; Fang, Wei; Liu, Kevin; Jau, Jack; Wang, Lester; Wan, Alex; Hunsche, Stefan; Halder, Sandip; Leray, Philippe

    2016-03-01

    As design rules for leading edge devices have shrunk to 1x nm size and below, device patterns have become sensitive to sub-10nm size defects. Additionally, defectivity and yield are now increasingly dominated by systematic patterning defects. A method for identifying and inspecting these hot spot (HS) locations is necessary for both technology development and High Volume Manufacturing (HVM). In order to achieve sufficient statistical significance across the wafer for a specific product and layer, a guided, high-speed e-beam inspection system is needed to cover a significant amount of high-volume hot spot locations for process window monitoring. In this paper, we explore the capabilities of a novel, highthroughput e-beam hot spot inspection tool, SkyScanTM 5000, on a 10nm back-end-of-line (BEOL) wafer patterned using a triple lithography-etch process. ASML's high-resolution, design-aware computational hot spot inspection is used to identify relevant hot spot locations, including overlay-sensitive patterns. We guide the e-beam tool to these Points of Interest (POI) and obtain experimental data from inspection of 430k wafer locations. The large amount of data allows detection of wafer-level and intra-field defect signatures for a large number of hot spot patterns.

  18. Identification of environmental anomaly hot spots in West Africa from time series of NDVI and rainfall

    Science.gov (United States)

    Boschetti, Mirco; Nutini, Francesco; Brivio, Pietro Alessandro; Bartholomé, Etienne; Stroppiana, Daniela; Hoscilo, Agata

    2013-04-01

    Studies of the impact of human activity on vegetation dynamics of the Sahelian belt of Africa have been recently re-invigorated by new scientific findings that highlighted the primary role of climate in the drought crises of the 1970s-1980s. Time series of satellite observations revealed a re-greening of the Sahelian belt that indicates no noteworthy human effect on vegetation dynamics at sub continental scale from the 1980s to late 1990s. However, several regional/local crises related to natural resources occurred in the last decades despite the re-greening thus underlying that more detailed studies are needed. In this study we used time-series (1998-2010) of SPOT-VGT NDVI and FEWS-RFE rainfall estimates to analyse vegetation - rainfall correlation and to map areas of local environmental anomalies where significant vegetation variations (increase/decrease) are not fully explained by seasonal changes of rainfall. Some of these anomalous zones (hot spots) were further analysed with higher resolution images Landsat TM/ETM+ to evaluate the reliability of the identified anomalous behaviour and to provide an interpretation of some example hot spots. The frequency distribution of the hot spots among the land cover classes of the GlobCover map shows that increase in vegetation greenness is mainly located in the more humid southern part and close to inland water bodies where it is likely to be related to the expansion/intensification of irrigated agricultural activities. On the contrary, a decrease in vegetation greenness occurs mainly in the northern part (12°-15°N) in correspondence with herbaceous vegetation covers where pastoral and cropping practices are often critical due to low and very unpredictable rainfall. The results of this study show that even if a general positive re-greening due to increased rainfall is evident for the entire Sahel, some local anomalous hot spots exist and can be explained by human factors such as population growth whose level reaches the

  19. Metatranscriptomic Analysis Reveals Unexpectedly Diverse Microbial Metabolism in a Biogeochemical Hot Spot in an Alluvial Aquifer

    Science.gov (United States)

    Jewell, Talia N. M.; Karaoz, Ulas; Bill, Markus; Chakraborty, Romy; Brodie, Eoin L.; Williams, Kenneth H.; Beller, Harry R.

    2017-01-01

    Organic matter deposits in alluvial aquifers have been shown to result in the formation of naturally reduced zones (NRZs), which can modulate aquifer redox status and influence the speciation and mobility of metals, affecting groundwater geochemistry. In this study, we sought to better understand how natural organic matter fuels microbial communities within anoxic biogeochemical hot spots (NRZs) in a shallow alluvial aquifer at the Rifle (CO) site. We conducted a 20-day microcosm experiment in which NRZ sediments, which were enriched in buried woody plant material, served as the sole source of electron donors and microorganisms. The microcosms were constructed and incubated under anaerobic conditions in serum bottles with an initial N2 headspace and were sampled every 5 days for metagenome and metatranscriptome profiles in combination with biogeochemical measurements. Biogeochemical data indicated that the decomposition of native organic matter occurred in different phases, beginning with mineralization of dissolved organic matter (DOM) to CO2 during the first week of incubation, followed by a pulse of acetogenesis that dominated carbon flux after 2 weeks. A pulse of methanogenesis co-occurred with acetogenesis, but only accounted for a small fraction of carbon flux. The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. This Hydrogenophaga strain also expressed genes indicative of chemolithoautotrophy, including CO2 fixation, H2 oxidation, S-compound oxidation, and denitrification. The pulse of acetogenesis appears to have been collectively catalyzed by a number of different organisms and metabolisms, most prominently pyruvate:ferredoxin oxidoreductase. Unexpected genes were identified among the most highly expressed

  20. Friction Stir Spot Welding of DP780 and Hot-Stamp Boron Steels

    Energy Technology Data Exchange (ETDEWEB)

    Santella, Michael L.; Frederick, Alan; Hovanski, Yuri; Grant, Glenn J.

    2008-05-16

    Friction stir spot welds were made in two high-strength steels: DP780, and a hot-stamp-boron steel with tensile strength of 1500 MPa. The spot welds were made at either 800 or 1600 rpm using either of two polycrystalline boron nitride tools. One stir tool, BN77, had the relatively common pin-tool shape. The second tool, BN46, had a convex rather than a concave shoulder profile and a much wider and shorter pin. The tools were plunged to preprogrammed depths either at a continuous rate (1-step schedule) or in two segments consisting of a relatively high rate followed by a slower rate. In all cases, the welds were completed in 4s. The range of lap-shear values were compared to values required for resistance spot welds on the same steels. The minimum value of 10.3 kN was exceeded for friction stir spot welding of DP780 using a 2-step schedule and either the BN77- or the BN46-type stir tool. The respective minimum value of 12 kN was also exceeded for the HSB steel using the 2-step process and the BN46 stir tool.

  1. Multiple modes of chromatin configuration at natural meiotic recombination hot spots in fission yeast.

    Science.gov (United States)

    Hirota, Kouji; Steiner, Walter W; Shibata, Takehiko; Ohta, Kunihiro

    2007-11-01

    The ade6-M26 meiotic recombination hot spot of fission yeast is defined by a cyclic AMP-responsive element (CRE)-like heptanucleotide sequence, 5'-ATGACGT-3', which acts as a binding site for the Atf1/Pcr1 heterodimeric transcription factor required for hot spot activation. We previously demonstrated that the local chromatin around the M26 sequence motif alters to exhibit higher sensitivity to micrococcal nuclease before the initiation of meiotic recombination. In this study, we have examined whether or not such alterations in chromatin occur at natural meiotic DNA double-strand break (DSB) sites in Schizosaccharomyces pombe. At one of the most prominent DSB sites, mbs1 (meiotic break site 1), the chromatin structure has a constitutively accessible configuration at or near the DSB sites. The establishment of the open chromatin state and DSB formation are independent of the CRE-binding transcription factor, Atf1. Analysis of the chromatin configuration at CRE-dependent DSB sites revealed both differences from and similarities to mbs1. For example, the tdh1+ locus, which harbors a CRE consensus sequence near the DSB site, shows a meiotically induced open chromatin configuration, similar to ade6-M26. In contrast, the cds1+ locus is similar to mbs1 in that it exhibits a constitutive open configuration. Importantly, Atf1 is required for the open chromatin formation in both tdh1+ and cds1+. These results suggest that CRE-dependent meiotic chromatin changes are intrinsic processes related to DSB formation in fission yeast meiosis. In addition, the results suggest that the chromatin configuration in natural meiotic recombination hot spots can be classified into at least three distinct categories: (i) an Atf1-CRE-independent constitutively open chromatin configuration, (ii) an Atf1-CRE-dependent meiotically induced open chromatin configuration, and (iii) an Atf1-CRE-dependent constitutively open chromatin configuration.

  2. A Healthcare Utilization Analysis Framework for Hot Spotting and Contextual Anomaly Detection

    Science.gov (United States)

    Hu, Jianying; Wang, Fei; Sun, Jimeng; Sorrentino, Robert; Ebadollahi, Shahram

    2012-01-01

    Patient medical records today contain vast amount of information regarding patient conditions along with treatment and procedure records. Systematic healthcare resource utilization analysis leveraging such observational data can provide critical insights to guide resource planning and improve the quality of care delivery while reducing cost. Of particular interest to providers are hot spotting: the ability to identify in a timely manner heavy users of the systems and their patterns of utilization so that targeted intervention programs can be instituted, and anomaly detection: the ability to identify anomalous utilization cases where the patients incurred levels of utilization that are unexpected given their clinical characteristics which may require corrective actions. Past work on medical utilization pattern analysis has focused on disease specific studies. We present a framework for utilization analysis that can be easily applied to any patient population. The framework includes two main components: utilization profiling and hot spotting, where we use a vector space model to represent patient utilization profiles, and apply clustering techniques to identify utilization groups within a given population and isolate high utilizers of different types; and contextual anomaly detection for utilization, where models that map patient’s clinical characteristics to the utilization level are built in order to quantify the deviation between the expected and actual utilization levels and identify anomalies. We demonstrate the effectiveness of the framework using claims data collected from a population of 7667 diabetes patients. Our analysis demonstrates the usefulness of the proposed approaches in identifying clinically meaningful instances for both hot spotting and anomaly detection. In future work we plan to incorporate additional sources of observational data including EMRs and disease registries, and develop analytics models to leverage temporal relationships among

  3. Using spatial statistics to identify emerging hot spots of forest loss

    Science.gov (United States)

    Harris, Nancy L.; Goldman, Elizabeth; Gabris, Christopher; Nordling, Jon; Minnemeyer, Susan; Ansari, Stephen; Lippmann, Michael; Bennett, Lauren; Raad, Mansour; Hansen, Matthew; Potapov, Peter

    2017-02-01

    As sources of data for global forest monitoring grow larger, more complex and numerous, data analysis and interpretation become critical bottlenecks for effectively using them to inform land use policy discussions. Here in this paper, we present a method that combines big data analytical tools with Emerging Hot Spot Analysis (ArcGIS) to identify statistically significant spatiotemporal trends of forest loss in Brazil, Indonesia and the Democratic Republic of Congo (DRC) between 2000 and 2014. Results indicate that while the overall rate of forest loss in Brazil declined over the 14-year time period, spatiotemporal patterns of loss shifted, with forest loss significantly diminishing within the Amazonian states of Mato Grosso and Rondônia and intensifying within the cerrado biome. In Indonesia, forest loss intensified in Riau province in Sumatra and in Sukamara and West Kotawaringin regencies in Central Kalimantan. Substantial portions of West Kalimantan became new and statistically significant hot spots of forest loss in the years 2013 and 2014. Similarly, vast areas of DRC emerged as significant new hot spots of forest loss, with intensified loss radiating out from city centers such as Beni and Kisangani. While our results focus on identifying significant trends at the national scale, we also demonstrate the scalability of our approach to smaller or larger regions depending on the area of interest and specific research question involved. When combined with other contextual information, these statistical data models can help isolate the most significant clusters of loss occurring over dynamic forest landscapes and provide more coherent guidance for the allocation of resources for forest monitoring and enforcement efforts.

  4. The meaning of the UHECR Hot Spots: A Light Nuclei Nearby Astronomy

    CERN Document Server

    Fargion, Daniele

    2014-01-01

    We update all the recent UHECR events reported by AUGER, Telescope Array as well as by AGASA in common coordinate maps. We confirm our earliest (2008-2013) model where UHECR are mostly light nuclei (He,Be,B), explaining the Virgo absence and confirming M82 as the main source of North TA Hot Spot. Many more sources , as NGC 253 and galactic ones, are possibly source of most of the 376 UHECR events. Several correlated map, already considered in recent years, are shown with all the events

  5. Hot spot analysis applied to identify ecosystem services potential in Lithuania

    Science.gov (United States)

    Pereira, Paulo; Depellegrin, Daniel; Misiune, Ieva

    2016-04-01

    Hot spot analysis are very useful to identify areas with similar characteristics. This is important for a sustainable use of the territory, since we can identify areas that need to be protected, or restored. This is a great advantage in terms of land use planning and management, since we can allocate resources, reduce the economical costs and do a better intervention in the landscape. Ecosystem services (ES) are different according land use. Since landscape is very heterogeneous, it is of major importance understand their spatial pattern and where are located the areas that provide better ES and the others that provide less services. The objective of this work is to use hot-spot analysis to identify areas with the most valuable ES in Lithuania. CORINE land-cover (CLC) of 2006 was used as the main spatial information. This classification uses a grid of 100 m resolution and extracted a total of 31 land use types. ES ranking was carried out based on expert knowledge. They were asked to evaluate the ES potential of each different CLC from 0 (no potential) to 5 (very high potential). Hot spot analysis were evaluated using the Getis-ord test, which identifies cluster analysis available in ArcGIS toolbox. This tool identifies areas with significantly high low values and significant high values at a p level of 0.05. In this work we used hot spot analysis to assess the distribution of providing, regulating cultural and total (sum of the previous 3) ES. The Z value calculated from Getis-ord was used to statistical analysis to access the clusters of providing, regulating cultural and total ES. ES with high Z value show that they have a high number of cluster areas with high potential of ES. The results showed that the Z-score was significantly different among services (Kruskal Wallis ANOVA =834. 607, pcultural (0.080±1.979) and regulating (0.076±1.961). These results suggested that providing services are more clustered than the remaining. Ecosystem Services Z score were

  6. Hot spots of mercury methylation in northern peatlands : spatial and seasonal variability

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, C.P.J.; Branfireun, B.A. [Toronto Univ., ON (Canada). Dept. of Geography; Heyes, A. [Maryland Univ., Solomons, MD (United States). Center for Environmental Science, Chesapeake Biological Lab; Kolka, R.K. [USDA Forest Service, North Central Research Station, Grand Rapids, MN (United States)

    2006-07-01

    Studies have shown that peatlands are an important sources of methylmercury (MeHg) to downstream aquatic ecosystems at the watershed scale. Hot spots of overly high MeHg concentration have been found to occur periodically in zones of groundwater upwelling. However, the spatiotemporal distribution, size, and importance of these MeHg hot spots to peatland MeHg export are not currently understood. For that reason, peat pore waters were sampled extensively throughout 4 small, northern peatlands in order to assess the spatial patterns of total mercury (HgT) and MeHg. The transferability of findings among peatlands was also assessed. Sampling took place during the spring, mid-summer and fall of 2005 at 2 peatlands in north central Minnesota and 2 in northwestern Ontario. In addition to this spatial survey, 4 high-resolution sampling grids were also established at the Minnesota sites in order to investigate the size of hot spots and the effects of upland runoff. In all cases, spatial variability in pore water MeHg concentration was much higher than the variability in HgT concentration, with standard deviations typically exceeding mean values. The spatial pattern of pore water MeHg concentration was characterized into the following 2 distinct zones: (1) the upland-peatland interface, where MeHg concentrations higher than 2 ng/L occur consistently, (2) the peatland interior, where concentrations rarely exceed 0.5 ng/L. In addition, extremely high concentrations of up to 12 ng/L were only found at the upland-peatland interface. These values corresponded to MeHg:HgT ratios greater than 65 per cent, while these ratios rarely exceeded 5 to 10 per cent elsewhere. It was concluded that these hot spots can be attributed to the limited reactants in zones where the in-situ biogeochemical milieu is conducive to mercury methylation. The uniform patterns noted across the peatlands indicate that spatial patterns of HgT and MeHg are important for mechanistic modelling and landscape

  7. Detection of spatial hot spots and variation for the neon flying squid Ommastrephes bartramii resources in the northwest Pacific Ocean

    Science.gov (United States)

    Feng, Yongjiu; Chen, Xinjun; Liu, Yan

    2016-08-01

    With the increasing effects of global climate change and fishing activities, the spatial distribution of the neon flying squid (Ommastrephes bartramii) is changing in the traditional fishing ground of 150°-160°E and 38°-45°N in the northwest Pacific Ocean. This research aims to identify the spatial hot and cold spots (i.e. spatial clusters) of O. bartramii to reveal its spatial structure using commercial fishery data from 2007 to 2010 collected by Chinese mainland squid-jigging fleets. A relatively strongly-clustered distribution for O. bartramii was observed using an exploratory spatial data analysis (ESDA) method. The results show two hot spots and one cold spot in 2007 while only one hot and one cold spots were identified each year from 2008 to 2010. The hot and cold spots in 2007 occupied 8.2% and 5.6% of the study area, respectively; these percentages for hot and cold spot areas were 5.8% and 3.1% in 2008, 10.2% and 2.9% in 2009, and 16.4% and 11.9% in 2010, respectively. Nearly half (>45%) of the squid from 2007 to 2009 reported by Chinese fleets were caught in hot spot areas while this percentage reached its peak at 68.8% in 2010, indicating that the hot spot areas are central fishing grounds. A further change analysis shows the area centered at 156°E/43.5°N was persistent as a hot spot over the whole period from 2007 to 2010. Furthermore, the hot spots were mainly identified in areas with sea surface temperature (SST) in the range of 15-20°C around warm Kuroshio Currents as well as with the chlorophyll-a (chl-a) concentration above 0.3 mg/m3. The outcome of this research improves our understanding of spatiotemporal hotspots and its variation for O. bartramii and is useful for sustainable exploitation, assessment, and management of this squid.

  8. Detection of spatial hot spots and variation for the neon flying squid Ommastrephes bartramii resources in the northwest Pacific Ocean

    Science.gov (United States)

    Feng, Yongjiu; Chen, Xinjun; Liu, Yan

    2017-07-01

    With the increasing effects of global climate change and fishing activities, the spatial distribution of the neon flying squid ( Ommastrephes bartramii) is changing in the traditional fishing ground of 150°-160°E and 38°-45°N in the northwest Pacific Ocean. This research aims to identify the spatial hot and cold spots (i.e. spatial clusters) of O. bartramii to reveal its spatial structure using commercial fishery data from 2007 to 2010 collected by Chinese mainland squid-jigging fleets. A relatively strongly-clustered distribution for O. bartramii was observed using an exploratory spatial data analysis (ESDA) method. The results show two hot spots and one cold spot in 2007 while only one hot and one cold spots were identified each year from 2008 to 2010. The hot and cold spots in 2007 occupied 8.2% and 5.6% of the study area, respectively; these percentages for hot and cold spot areas were 5.8% and 3.1% in 2008, 10.2% and 2.9% in 2009, and 16.4% and 11.9% in 2010, respectively. Nearly half (>45%) of the squid from 2007 to 2009 reported by Chinese fleets were caught in hot spot areas while this percentage reached its peak at 68.8% in 2010, indicating that the hot spot areas are central fishing grounds. A further change analysis shows the area centered at 156°E/43.5°N was persistent as a hot spot over the whole period from 2007 to 2010. Furthermore, the hot spots were mainly identified in areas with sea surface temperature (SST) in the range of 15-20°C around warm Kuroshio Currents as well as with the chlorophyll- a (chl- a) concentration above 0.3 mg/m3. The outcome of this research improves our understanding of spatiotemporal hotspots and its variation for O. bartramii and is useful for sustainable exploitation, assessment, and management of this squid.

  9. A novel X-ray spectrometer for plasma hot spot diagnosis

    Science.gov (United States)

    Shi, Jun; Guo, Yongchao; Xiao, Shali; Yang, Zuhua; Qian, Feng; Cao, LeiFeng; Gu, Yuqiu

    2017-09-01

    A novel X-ray spectrometer is designed to diagnose the different conditions in plasmas. It can provide both X-ray spectroscopy and plasma image information simultaneously. Two pairs of elliptical crystal analyzers are used to measure the X-ray spectroscopy in the range of 2-20 keV. The pinhole imaging system coupled with gated micro-channel plate(MCP) detectors are developed, which allows 20 images to be collected in a single individual experiment. The experiments of measuring spectra were conducted at ;Shenguang-II upgraded laser; in China Academy of Engineering Physics to demonstrate the utility of the spectrometer. The X-ray spectroscopy information was obtained by the image plate(IP). The hot spot imaging experiments were carried out at ;Shenguang-III prototype facility;. We have obtained the hot sport images with the spectrometer, and the signal to noise ratio of 30 ∼ 40 is observed.

  10. Uranium Bioreduction Rates across Scales: Biogeochemical Hot Moments and Hot Spots during a Biostimulation Experiment at Rifle, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Bao, Chen; Wu, Hongfei; Li, Li; Newcomer, Darrell R.; Long, Philip E.; Williams, Kenneth H.

    2014-09-02

    We aim to understand the scale-dependent evolution of uranium bioreduction during a field experiment at a former uranium mill site near Rifle, Colorado. Acetate was injected to stimulate Fe-reducing bacteria (FeRB) and to immobilize aqueous U(VI) to insoluble U(IV). Bicarbonate was coinjected in half of the domain to mobilize sorbed U(VI). We used reactive transport modeling to integrate hydraulic and geochemical data and to quantify rates at the grid block (0.25 m) and experimental field scale (tens of meters). Although local rates varied by orders of magnitude in conjunction with biostimulation fronts propagating downstream, field-scale rates were dominated by those orders of magnitude higher rates at a few selected hot spots where Fe(III), U(VI), and FeRB were at their maxima in the vicinity of the injection wells. At particular locations, the hot moments with maximum rates negatively corresponded to their distance from the injection wells. Although bicarbonate injection enhanced local rates near the injection wells by a maximum of 39.4%, its effect at the field scale was limited to a maximum of 10.0%. We propose a rate-versus-measurement-length relationship (log R' = -0.63

  11. Focal hot spot induced by a central subclavian line on bone scan

    Directory of Open Access Journals (Sweden)

    Masood Moslehi

    2014-01-01

    Full Text Available The diagnostic accuracy of nuclear medicine reporting can be improved by awareness of these instrument-related artifacts. Both awareness and experience are also important when it comes to detecting and identifying normal (and abnormal variants. We present a case of hot spot on the upper right chest in the region of right subclavicular region resulting from injection of radiotracer from central subclavian line. A 52-year-old woman with a history of left breast cancer and recent bone pain was referred to our nuclear medicine department for skeletal survey. Anterior views of chest show a focus of increased radiotracer uptake corresponding to anterior arch of one of the right second rib. The nuclear physician reported it as a focal rib bony lesion and recommended radiological evaluation. As technician later explained, physicians realized that injection site was a central subclavian line on the right side and hot spot on that region is due to injection site. The appearance of both skeletal and soft-tissue uptake depends heavily on imaging technique (such as the route of radiotracer administration and the interpreting physicians should be aware of the impact of technical factors on image quality.

  12. VLBI observations of 10 CSO candidates: expansion velocities of hot spots

    CERN Document Server

    An, Tao; Yang, Jun; Taylor, Gregory B; Hong, Xiaoyu; Baan, Willem A; Liu, Xiang; Wang, Min; Zhang, Hongbo; Wang, Weihua; Chen, Xi; Cui, Lang; Hao, Longfei; Zhu, Xinying

    2011-01-01

    Observations of ten Compact Symmetric Objects ({\\rm CSO}) candidates have been made with the Very Long Baseline Array at 8.4 GHz in 2005 and with a combined Chinese and European VLBI array at 8.4 GHz in 2009. The 2009 observations incorporate for the first time the two new Chinese telescopes at Miyun and Kunming for international astrophysical observations. The observational data, in combination with archival VLBA data from previous epochs, have been used to derive the proper motions of the VLBI components. Because of the long time baseline of $\\sim$16 years of the VLBI data sets, the expansion velocities of the hot spots can be measured at an accuracy as high as $\\sim$1.3 $\\mu$as yr$^{-1}$. Six of the ten sources are identified as CSOs with a typical double or triple morphology on the basis of both spectral index maps and their mirror-symmetry of proper motions of the terminal hot spots. The compact double source J1324+4048 is also identified as a CSO candidate. Among the three remaining sources, J1756+5748 ...

  13. Possible hot spots excited by the relativistic jets of Cygnus X-3

    CERN Document Server

    Martí, J; Garrido, J L; Luque-Escamilla, P; Paredes, J M

    2005-01-01

    We present the results of a deep search for associated radio features in the vicinity of the microquasar Cygnus X-3. The motivation behind is to find out evidence for interaction between its relativistic jets and the surrounding interstellar medium, which could eventually allow us to perform calorimetry of the total energy released by this microquasar during its flaring lifetime. Remarkably, two radio sources with mJy emission level at centimeter wavelengths have been detected in excellent alignment with the position angle of the inner radio jets. We propose that these objects could be the hot spots where the relativitic outflow collides with the ambient gas in analogy with Fanaroff-Riley II radio galaxies. These candidate hot spots are within a few arc-minutes of Cygnus X-3 and, if physically related, the full linear extent of the jet would reach tens of parsecs. We discuss here the evidence currently available to support this hypothesis based on both archival data and our own observations.

  14. Rapid experimental SAD phasing and hot-spot identification with halogenated fragments

    Energy Technology Data Exchange (ETDEWEB)

    Bauman, Joseph D.; Harrison, Jerry Joe E. K.; Arnold, Eddy

    2016-01-01

    Through X-ray crystallographic fragment screening, 4-bromopyrazole was discovered to be a `magic bullet' that is capable of binding at many of the ligand `hot spots' found in HIV-1 reverse transcriptase (RT). The binding locations can be in pockets that are `hidden' in the unliganded crystal form, allowing rapid identification of these sites forin silicoscreening. In addition to hot-spot identification, this ubiquitous yet specific binding provides an avenue for X-ray crystallographic phase determination, which can be a significant bottleneck in the determination of the structures of novel proteins. The anomalous signal from 4-bromopyrazole or 4-iodopyrazole was sufficient to determine the structures of three proteins (HIV-1 RT, influenza A endonuclease and proteinase K) by single-wavelength anomalous dispersion (SAD) from single crystals. Both compounds are inexpensive, readily available, safe and very soluble in DMSO or water, allowing efficient soaking into crystals.

  15. Grain-Scale Simulations of Hot-Spot Initiation for Shocked TATB

    Energy Technology Data Exchange (ETDEWEB)

    Najjar, F; Howard, W; Fried, L

    2009-07-31

    High-explosive (HE) material consists of large-sized grains with micron-sized embedded impurities and pores. Under various mechanical/thermal insults, these pores collapse generating high-temperature regions leading to ignition. A computational study has been performed to investigate the mechanisms of pore collapse and hot spot initiation in TATB crystals, employing the thermohydrodynamics arbitrary-Lagrange-Eulerian code ALE3D. This initial study includes non-reactive dynamics to isolate the thermal and hydrodynamical effects. Two-dimensional high-resolution large-scale meso-scale simulations have been undertaken. We study an axisymmetric configuration for pore radii ranging from 0.5 to 2{micro}m, with initial shock pressures in the range from 3 to 11 GPa. A Mie-Gruneisen Equation of State (EOS) model is used for TATB, and includes a constant yield strength and shear modulus; while the air in the pore invokes a Livermore Equation of State (LEOS) model. The parameter space is systematically studied by considering various shock strengths, pore diameters and material properties. We find that thermal diffusion from the collapsed pores has an important effect in generating high-temperature hot spots in the TATB.

  16. Computer animation of hot spot development in bulk coal as an aid for training coal miners

    Energy Technology Data Exchange (ETDEWEB)

    Hancock, M.; Kizil, M.S.; Beamish, B.B. [University of Queensland, Brisbane, Qld. (Australia). School of Engineering

    2005-07-01

    The processes that take place during the development of a heating are difficult to visualise. Bulk coal self-heating tests at The University of Queensland (UQ) using a two-metre column are providing graphic evidence of the stages that occur during a heating. Data obtained from these tests, both temperature and corresponding off-gas evolution can be transformed into what is effectively a video-replay of the heating event. This is achieved by loading both sets of data into a newly developed animation package called Hotspot. The resulting animation is ideal for spontaneous combustion training purposes as the viewer can readily identify the different hot spot stages and corresponding off-gas signatures. Colour coding of the coal temperature, as the hot spot forms, highlights its location in the coal pile and shows its ability to migrate upwind. An added benefit of the package is that once a mine has been tested in the UQ two-metre column, there is a permanent record of that particular coals performance for mine personnel to view. 7 refs., 9 figs., 1 tab.

  17. Hot-spot tectonics of Eistla Regio, Venus: Results from Magellan images and Pioneer Venus gravity

    Science.gov (United States)

    Grimm, Robert E.; Phillips, Roger J.

    1991-06-01

    Eistla Regio (ER) is a broad, low, discontinuous topographic rise striking roughly EW at low northern latitudes of Venus. Some 2000 x 7000 km in dimensions, it is the third largest rise in planform on Venus after Aphrodite Terra and Beta Phoebe Regiones. These rises are the key physiographic elements in a hot spot model of global tectonics including transient plume behavior. Since ER is the first such rise viewed by Magellan and the latitude is very favorable for Pioneer Venus gravity studies, some of the predictions of a time dependent hot spot model are tested. Western ER is defined as the rise including Gula and Sif Mons and central ER as that including Sappho Patera. Superior conjunction prevented Magellan from returning data on eastern ER (Pavlova) during the first mapping cycle. It is concluded that the western and central portions of ER, while part of the same broad topographic rise and tectonic framework, have distinctly different surface ages and gravity signatures. The western rise, including Gula and Sif Mons, is the expression of deep seated uplift with volcanism limited to the individual large shields. The eastern portion has been widely resurfaced more recently by thermal anomalies in the mantle.

  18. Thermal analysis of continuous and patterned multilayer films in the presence of a nanoscale hot spot

    Science.gov (United States)

    Juang, Jia-Yang; Zheng, Jinglin

    2016-10-01

    Thermal responses of multilayer films play essential roles in state-of-the-art electronic systems, such as photo/micro-electronic devices, data storage systems, and silicon-on-insulator transistors. In this paper, we focus on the thermal aspects of multilayer films in the presence of a nanoscale hot spot induced by near field laser heating. The problem is set up in the scenario of heat assisted magnetic recording (HAMR), the next-generation technology to overcome the data storage density limit imposed by superparamagnetism. We characterized thermal responses of both continuous and patterned multilayer media films using transient thermal modeling. We observed that material configurations, in particular, the thermal barriers at the material layer interfaces crucially impact the temperature field hence play a key role in determining the hot spot geometry, transient response and power consumption. With a representative generic media model, we further explored the possibility of optimizing thermal performances by designing layers of heat sink and thermal barrier. The modeling approach demonstrates an effective way to characterize thermal behaviors of micro and nano-scale electronic devices with multilayer thin film structures. The insights into the thermal transport scheme will be critical for design and operations of such electronic devices.

  19. A conserved TLR5 binding and activation hot spot on flagellin

    Science.gov (United States)

    Song, Wan Seok; Jeon, Ye Ji; Namgung, Byeol; Hong, Minsun; Yoon, Sung-il

    2017-01-01

    Flagellin is a bacterial protein that polymerizes into the flagellar filament and is essential for bacterial motility. When flagellated bacteria invade the host, flagellin is recognized by Toll-like receptor 5 (TLR5) as a pathogen invasion signal and eventually evokes the innate immune response. Here, we provide a conserved structural mechanism by which flagellins from Gram-negative γ-proteobacteria and Gram-positive Firmicutes bacteria bind and activate TLR5. The comparative structural analysis using our crystal structure of a complex between Bacillus subtilis flagellin (bsflagellin) and TLR5 at 2.1 Å resolution, combined with the alanine scanning analysis of the binding interface, reveals a common hot spot in flagellin for TLR5 activation. An arginine residue (bsflagellin R89) of the flagellin D1 domain and its adjacent residues (bsflagellin E114 and L93) constitute a hot spot that provides shape and chemical complementarity to a cavity generated by the loop of leucine-rich repeat 9 in TLR5. In addition to the flagellin D1 domain, the D0 domain also contributes to TLR5 activity through structurally dispersed regions, but not a single focal area. These results establish the groundwork for the future design of flagellin-based therapeutics. PMID:28106112

  20. Thermal analysis of continuous and patterned multilayer films in the presence of a nanoscale hot spot

    Directory of Open Access Journals (Sweden)

    Jia-Yang Juang

    2016-10-01

    Full Text Available Thermal responses of multilayer films play essential roles in state-of-the-art electronic systems, such as photo/micro-electronic devices, data storage systems, and silicon-on-insulator transistors. In this paper, we focus on the thermal aspects of multilayer films in the presence of a nanoscale hot spot induced by near field laser heating. The problem is set up in the scenario of heat assisted magnetic recording (HAMR, the next-generation technology to overcome the data storage density limit imposed by superparamagnetism. We characterized thermal responses of both continuous and patterned multilayer media films using transient thermal modeling. We observed that material configurations, in particular, the thermal barriers at the material layer interfaces crucially impact the temperature field hence play a key role in determining the hot spot geometry, transient response and power consumption. With a representative generic media model, we further explored the possibility of optimizing thermal performances by designing layers of heat sink and thermal barrier. The modeling approach demonstrates an effective way to characterize thermal behaviors of micro and nano-scale electronic devices with multilayer thin film structures. The insights into the thermal transport scheme will be critical for design and operations of such electronic devices.

  1. Sensitivity of inertial confinement fusion hot spot properties to the deuterium-tritium fuel adiabat

    Energy Technology Data Exchange (ETDEWEB)

    Melvin, J.; Lim, H.; Rana, V.; Glimm, J. [Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, New York 11794-3600 (United States); Cheng, B.; Sharp, D. H.; Wilson, D. C. [Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)

    2015-02-15

    We determine the dependence of key Inertial Confinement Fusion (ICF) hot spot simulation properties on the deuterium-tritium fuel adiabat, here modified by addition of energy to the cold shell. Variation of this parameter reduces the simulation to experiment discrepancy in some, but not all, experimentally inferred quantities. Using simulations with radiation drives tuned to match experimental shots N120321 and N120405 from the National Ignition Campaign (NIC), we carry out sets of simulations with varying amounts of added entropy and examine the sensitivities of important experimental quantities. Neutron yields, burn widths, hot spot densities, and pressures follow a trend approaching their experimentally inferred quantities. Ion temperatures and areal densities are sensitive to the adiabat changes, but do not necessarily converge to their experimental quantities with the added entropy. This suggests that a modification to the simulation adiabat is one of, but not the only explanation of the observed simulation to experiment discrepancies. In addition, we use a theoretical model to predict 3D mix and observe a slight trend toward less mixing as the entropy is enhanced. Instantaneous quantities are assessed at the time of maximum neutron production, determined dynamically within each simulation. These trends contribute to ICF science, as an effort to understand the NIC simulation to experiment discrepancy, and in their relation to the high foot experiments, which features a higher adiabat in the experimental design and an improved neutron yield in the experimental results.

  2. Hot spots and labyrinths: Why cortical neuromodulation for episodic migraine with aura should be personalized

    Directory of Open Access Journals (Sweden)

    Markus A Dahlem

    2015-03-01

    Full Text Available Stimulation protocols for medical devices should be rationally designed. For episodic migraine with aura we outline model-based design strategies towards preventive and acute therapies using stereotactic cortical neuromodulation. To this end, we regard a localized spreading depression (SD wave segment as a central element in migraine pathophysiology. To describe nucleation and propagation features of the SD wave segment, we define the new concepts of cortical hot spots and labyrinths, respectively. In particular, we firstly focus exclusively on curvature-induced dynamical properties by studying a generic reaction-diffusion model of SD on the folded cortical surface. This surface is described with increasing level of details, including finally personalized simulations using patient's magnetic resonance imaging (MRI scanner readings. At this stage, the only relevant factor that can modulate nucleation and propagation paths is the Gaussian curvature, which has the advantage of being rather readily accessible by MRI. We conclude with discussing further anatomical factors, such as areal, laminar, and cellular heterogeneity, that in addition to and in relation to Gaussian curvature determine the generalized concept of cortical hot spots and labyrinths as target structures for neuromodulation. Our numerical simulations suggest that these target structures are like fingerprints, they are individual features of each migraine sufferer. The goal in the future will be to provide individualized neural tissue simulations. These simulations should predict the clinical data and therefore can also serve as a test bed for exploring stereotactic cortical neuromodulation.

  3. Biofilm models for the food industry: hot spots for plasmid transfer?

    Science.gov (United States)

    Van Meervenne, Eva; De Weirdt, Rosemarie; Van Coillie, Els; Devlieghere, Frank; Herman, Lieve; Boon, Nico

    2014-04-01

    Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-²)] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.

  4. Hot spots and future directions of research on the neuroprotective effects of nimodipine

    Institute of Scientific and Technical Information of China (English)

    Runhui Li

    2014-01-01

    Calcium antagonists are widely used in the clinical treatment of ischemic cerebrovascular disease because of their vascular and neuroprotective effects. Nimodipine, a typical calcium antagonist, can cross the blood-brain barrier and act selectively at neurons and blood vessels of target tis-sues, thus exerting neuroprotective effects. The aim of the present study was to explore the hot spots and future trends of research on the neuroprotective effects of nimodipine. We retrieved 425 articles on the neuroprotective effects of nimodipine that were indexed in the Web of the Science database between 2000 and 2014. The retrieved articles were analyzed using document analysis reporting and the derived information function in the Web of Science, and the infor-mation visualization software CiteSpace III. The reference co-citation network was plotted, and the high frequency key words in these publications were used to analyze the research fronts and development trends for nimodipine neuroprotection. According to these co-citation clusters, the research front of nimodipine neuroprotection is the use of randomized controlled trials to study nimodipine intervention of subarachnoid hemorrhage. Using time zone view analysis on hot spots labeled with a key word, the areas of interest in the ifeld of nimodipine neuroprotection are nimodipine pharmacology and therapeutics, blood-brain barrier, trials, and anti-angiospasm.

  5. "Atomic Force Masking" Induced Formation of Effective Hot Spots along Grain Boundaries of Metal Thin Films.

    Science.gov (United States)

    Kim, Kwang Hyun; Chae, Soo Sang; Jang, Seunghun; Choi, Won Jin; Chang, Hyunju; Lee, Jeong-O; Lee, Tae Il

    2016-11-30

    We present an interesting phenomenon, "atomic force masking", which is the deposition of a few-nanometer-thick gold film on ultrathin low-molecular-weight (LMW) polydimethylsiloxane (PDMS) engineered on a polycrystalline gold thin film, and demonstrated the formation of hot spot based on SERS. The essential principle of this atomic force masking phenomenon is that an LMW PDMS layer on a single crystalline grain of gold thin film would repel gold atoms approaching this region during a second cycle of evaporation, whereas new nucleation and growth of gold atoms would occur on LMW PDMS deposited on grain boundary regions. The nanostructure formed by the atomic force masking, denoted here as "hot spots on grain boundaries" (HOGs), which is consistent with finite-difference time-domain (FDTD) simulation, and the mechanism of atomic force masking were investigated by carrying out systematic experiments, and density functional theory (DFT) calculations were made to carefully explain the related fundamental physics. Also, to highlight the manufacturing advantages of the proposed method, we demonstrated the simple synthesis of a flexible HOG SERS, and we used this substrate in a swabbing test to detect a common pesticide placed on the surface of an apple.

  6. Lack of mutational hot spots during decitabine-mediated HIV-1 mutagenesis.

    Science.gov (United States)

    Rawson, Jonathan M O; Landman, Sean R; Reilly, Cavan S; Bonnac, Laurent; Patterson, Steven E; Mansky, Louis M

    2015-11-01

    Decitabine has previously been shown to induce lethal mutagenesis of human immunodeficiency virus type 1 (HIV-1). However, the factors that determine the susceptibilities of individual sequence positions in HIV-1 to decitabine have not yet been defined. To investigate this, we performed Illumina high-throughput sequencing of multiple amplicons prepared from proviral DNA that was recovered from decitabine-treated cells infected with HIV-1. We found that decitabine induced an ≈4.1-fold increase in the total mutation frequency of HIV-1, primarily due to a striking ≈155-fold increase in the G-to-C transversion frequency. Intriguingly, decitabine also led to an ≈29-fold increase in the C-to-G transversion frequency. G-to-C frequencies varied substantially (up to ≈80-fold) depending upon sequence position, but surprisingly, mutational hot spots (defined as upper outliers within the mutation frequency distribution) were not observed. We further found that every single guanine position examined was significantly susceptible to the mutagenic effects of decitabine. Taken together, these observations demonstrate for the first time that decitabine-mediated HIV-1 mutagenesis is promiscuous and occurs in the absence of a clear bias for mutational hot spots. These data imply that decitabine-mediated G-to-C mutagenesis is a highly effective antiviral mechanism for extinguishing HIV-1 infectivity.

  7. Ignition conditions relaxation for central hot-spot ignition with an ion-electron non-equilibrium model

    Science.gov (United States)

    Fan, Zhengfeng; Liu, Jie

    2016-10-01

    We present an ion-electron non-equilibrium model, in which the hot-spot ion temperature is higher than its electron temperature so that the hot-spot nuclear reactions are enhanced while energy leaks are considerably reduced. Theoretical analysis shows that the ignition region would be significantly enlarged in the hot-spot rhoR-T space as compared with the commonly used equilibrium model. Simulations show that shocks could be utilized to create and maintain non-equilibrium conditions within the hot spot, and the hot-spot rhoR requirement is remarkably reduced for achieving self-heating. In NIF high-foot implosions, it is observed that the x-ray enhancement factors are less than unity, which is not self-consistent and is caused by assuming Te =Ti. And from this non-consistency, we could infer that ion-electron non-equilibrium exists in the high-foot implosions and the ion temperature could be 9% larger than the equilibrium temperature.

  8. Z-score biological significance of binding hot spots of protein interfaces by using crystal packing as the reference state.

    Science.gov (United States)

    Liu, Qian; Wong, Limsoon; Li, Jinyan

    2012-12-01

    Characterization of binding hot spots of protein interfaces is a fundamental study in molecular biology. Many computational methods have been proposed to identify binding hot spots. However, there are few studies to assess the biological significance of binding hot spots. We introduce the notion of biological significance of a contact residue for capturing the probability of the residue occurring in or contributing to protein binding interfaces. We take a statistical Z-score approach to the assessment of the biological significance. The method has three main steps. First, the potential score of a residue is defined by using a knowledge-based potential function with relative accessible surface area calculations. A null distribution of this potential score is then generated from artifact crystal packing contacts. Finally, the Z-score significance of a contact residue with a specific potential score is determined according to this null distribution. We hypothesize that residues at binding hot spots have big absolute values of Z-score as they contribute greatly to binding free energy. Thus, we propose to use Z-score to predict whether a contact residue is a hot spot residue. Comparison with previously reported methods on two benchmark datasets shows that this Z-score method is mostly superior to earlier methods. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.

  9. HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering.

    Science.gov (United States)

    Bendl, Jaroslav; Stourac, Jan; Sebestova, Eva; Vavra, Ondrej; Musil, Milos; Brezovsky, Jan; Damborsky, Jiri

    2016-07-01

    HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart libraries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evolutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four different protein engineering strategies and optimizing the resulting library's size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein's structure and visualized with a JSmol applet. HotSpot Wizard lists annotated residues suitable for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive annotations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries. It is freely available at http://loschmidt.chemi.muni.cz/hotspotwizard.

  10. KFC2: a knowledge-based hot spot prediction method based on interface solvation, atomic density, and plasticity features.

    Science.gov (United States)

    Zhu, Xiaolei; Mitchell, Julie C

    2011-09-01

    Hot spots constitute a small fraction of protein-protein interface residues, yet they account for a large fraction of the binding affinity. Based on our previous method (KFC), we present two new methods (KFC2a and KFC2b) that outperform other methods at hot spot prediction. A number of improvements were made in developing these new methods. First, we created a training data set that contained a similar number of hot spot and non-hot spot residues. In addition, we generated 47 different features, and different numbers of features were used to train the models to avoid over-fitting. Finally, two feature combinations were selected: One (used in KFC2a) is composed of eight features that are mainly related to solvent accessible surface area and local plasticity; the other (KFC2b) is composed of seven features, only two of which are identical to those used in KFC2a. The two models were built using support vector machines (SVM). The two KFC2 models were then tested on a mixed independent test set, and compared with other methods such as Robetta, FOLDEF, HotPoint, MINERVA, and KFC. KFC2a showed the highest predictive accuracy for hot spot residues (True Positive Rate: TPR = 0.85); however, the false positive rate was somewhat higher than for other models. KFC2b showed the best predictive accuracy for hot spot residues (True Positive Rate: TPR = 0.62) among all methods other than KFC2a, and the False Positive Rate (FPR = 0.15) was comparable with other highly predictive methods.

  11. Detection of direct and indirect noise generated by synthetic hot spots in a duct

    Science.gov (United States)

    De Domenico, Francesca; Rolland, Erwan O.; Hochgreb, Simone

    2017-04-01

    Sound waves in a combustor are generated from fluctuations in the heat release rate (direct noise) or the acceleration of entropy, vorticity or compositional perturbations through nozzles or turbine guide vanes (indirect or entropy noise). These sound waves are transmitted downstream as well as reflected upstream of the acceleration point, contributing to the overall noise emissions, or triggering combustion instabilities. Previous experiments attempted to isolate indirect noise by generating thermoacoustic hot spots electrically and measuring the transmitted acoustic waves, yet there are no measurements on the backward propagating entropy and acoustic waves. This work presents the first measurements which clearly separate the direct and indirect noise contributions to pressure fluctuations upstream of the acceleration point. Synthetic entropy spots are produced by unsteady electrical heating of a grid of thin wires located in a tube. Compression waves (direct noise) are generated from this heating process. The hot spots are then advected with the mean flow and finally accelerated through an orifice plate located at the end of the tube, producing a strong acoustic signature which propagates upstream (indirect noise). The convective time is selected to be longer than the heating pulse length, in order to obtain a clear time separation between direct and indirect noise in the overall pressure trace. The contribution of indirect noise to the overall noise is shown to be non-negligible either in subsonic or sonic throat conditions. However, the absolute amplitude of direct noise is larger than the corresponding fraction of indirect noise, explaining the difficulty in clearly identifying the two contributions when they are merged. Further, the work shows the importance of using appropriate pressure transducer instrumentation and correcting for the respective transfer functions in order to account for low frequency effects in the determination of pressure fluctuations.

  12. A reactive burn model for shock initiation in a PBX: scaling and separability based on the hot spot concept

    Energy Technology Data Exchange (ETDEWEB)

    Show, Milton S [Los Alamos National Laboratory; Menikoff, Ralph [Los Alamos National Laboratory

    2010-01-01

    In the formulation of a reactive burn model for shock initiation, we endeavor to incorporate a number of effects based on the underlying physical concept of hot spot ignition followed by the growth of reaction due to diverging deflagration fronts. The passage of a shock front sets the initial condition for reaction, leading to a fraction of the hot spots that completely burn while others will quench. The form of the rate model is chosen to incorporate approximations based on the physical picture. In particular, the approximations imply scaling relations that are then used to mathematically separate various contributions. That is, the model is modular and refinements can be applied separately without changing the other contributions. For example, the effect of initial temperature, porosity, etc. predominantly enter the characterization of the non-quenching hot spot distribution. A large collection of velocity gauge data is shown to be well represented by the model with a very small number of parameters.

  13. XMM-Newton observations of the hot spot galaxy NGC 2903

    CERN Document Server

    Perez-Ramirez, D; Ebrero, J; Leon, S

    2010-01-01

    We report on the first deeper X-ray broad-band observation of the hot spot galaxy NGC 2903 obtained with XMM-Newton. X-ray imaging and spectra of the spiral barred galaxy NGC 2903 were obtained from recently available XMM-Newton archival data in order to study its X-ray population and the conditions of the hot gas in its central region. We investigate the spectral properties for the discrete point-source population and give first estimations of their X-ray spectral parameters. By analysing the RGS spectra, we derive temperature and abundances for the hot gas located in its central region. A total of 6 X-ray point sources (4 of them ULX candidates) were detected in the energy range of 0.3-10.0 keV located within the galaxy D25 optical disk. 3 out of these sources are detected for the first time, and one of them, XMM-NGC2903 X2 with luminosity larger than 10^39 erg/s. After fitting three different models, we were able to estimate their luminosities which are compatible with binaries with a compact object in the...

  14. Assessing Statistically Significant Heavy-Metal Concentrations in Abandoned Mine Areas via Hot Spot Analysis of Portable XRF Data

    Directory of Open Access Journals (Sweden)

    Sung-Min Kim

    2017-06-01

    Full Text Available To develop appropriate measures to prevent soil contamination in abandoned mining areas, an understanding of the spatial variation of the potentially toxic trace elements (PTEs in the soil is necessary. For the purpose of effective soil sampling, this study uses hot spot analysis, which calculates a z-score based on the Getis-Ord Gi* statistic to identify a statistically significant hot spot sample. To constitute a statistically significant hot spot, a feature with a high value should also be surrounded by other features with high values. Using relatively cost- and time-effective portable X-ray fluorescence (PXRF analysis, sufficient input data are acquired from the Busan abandoned mine and used for hot spot analysis. To calibrate the PXRF data, which have a relatively low accuracy, the PXRF analysis data are transformed using the inductively coupled plasma atomic emission spectrometry (ICP-AES data. The transformed PXRF data of the Busan abandoned mine are classified into four groups according to their normalized content and z-scores: high content with a high z-score (HH, high content with a low z-score (HL, low content with a high z-score (LH, and low content with a low z-score (LL. The HL and LH cases may be due to measurement errors. Additional or complementary surveys are required for the areas surrounding these suspect samples or for significant hot spot areas. The soil sampling is conducted according to a four-phase procedure in which the hot spot analysis and proposed group classification method are employed to support the development of a sampling plan for the following phase. Overall, 30, 50, 80, and 100 samples are investigated and analyzed in phases 1–4, respectively. The method implemented in this case study may be utilized in the field for the assessment of statistically significant soil contamination and the identification of areas for which an additional survey is required.

  15. Multifocal hot spots demonstrated by whole-body 131I scintigraphy and SPECT/CT after transaxillary endoscopic thyroidectomy.

    Science.gov (United States)

    Kim, Ho Seong; Kim, Seok Hwi; Kim, Jung Han; Kim, Byung-Tae; Lee, Kyung-Han

    2015-03-01

    A 35-year-old woman received open thyroidectomy for a thyroid nodule that was confirmed as papillary carcinoma. Whole-body 131I scintigraphy during thyroid ablation demonstrated high uptake in the thyroid bed and multiple focal hot spots in the thorax. SPECT/CT localized the hot spots to the right chest wall and axilla, as well as to the left chest wall. The surgeon recognized these sites to concur with the transaxillary tract used during endoscopic thyroidectomy for nodular hyperplasia 8 years previously. Thus, this case illustrates how thyroidal tissue fragments seeded during endoscopic thyroidectomy can be mistaken for thyroid cancer metastasis on 131I scintigraphy.

  16. Identification of potential hot spots in the carboxy-terminal part of the Epstein-Barr virus (EBV) BNLF-1 gene in both malignant and benign EBV-associated diseases

    DEFF Research Database (Denmark)

    Sandvej, K; Peh, S C; Andresen, B S

    1994-01-01

    mononucleosis (IM). Our study showed that some of the 7 single-base mutations and the 30-bp deletion previously detected between codons of amino acid 322 and 366 in the BNLF-1 gene of the nasopharyngeal carcinoma cell line CAO were present in all Malaysian PTLs and in 60% of the Danish PTLs. In HD and the IM...

  17. Chain of impact of the development of hot spots and hot rubbing in disk brakes of passenger cars; Wirkungskette der Entstehung von Hotspots und Heissrubbeln in Pkw-Scheibenbremsen

    Energy Technology Data Exchange (ETDEWEB)

    Sarda, Angelo

    2009-07-01

    Aspects coupled with brake comfort represent challenges with the development of brake systems. During the brake process, so-called hot spots on the surface of brake disks are developed under certain conditions. These hot spots result in an irregular thermal expansion and deformation of the brake disk. This results in vibrations impacting the comfort and being known as 'hot rubbing'. The author of the contribution under consideration describes the most important results from extensive benchmark tests and supplies experimentally secured realizations to the chain of impact of the development of hot spots and 'hot rubbing' in a disc brake.

  18. Treatability studies on F/H Area ``hot spot`` groundwater composite. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Bibler, J.P.

    1993-08-30

    The data found in this report were collected from laboratory experiments that were conducted to characterize the ``hot spot`` groundwater before and after pH adjustment, to describe the settling behavior and particle size of the precipitates resulting from pH adjustment, and to compare several methods of pH adjustment. Although Decontamination Factors (DFs) for all precipitating agents are similar, the best settling characteristics and most manageable precipitate were produced when 25 ppM Al{sup 3+} was introduced as Al{sub 2}(SO{sub 4}){sub 3} and pH adjustment was made from 6--8 with NaOH. The resulting precipitate will not be a hazardous secondary waste.

  19. Analytical criterion for shock ignition of fusion reaction in hot spot

    Directory of Open Access Journals (Sweden)

    Ribeyre X.

    2013-11-01

    Full Text Available Shock ignition of DT capsules involves two major steps. First, the fuel is assembled by means of a low velocity conventional implosion. At stagnation, the central core has a temperature lower than the one needed for ignition. Then a second, strong spherical converging shock, launched from a high intensity laser spike, arrives to the core. This shock crosses the core, rebounds at the target center and increases the central pressure to the ignition conditions. In this work we consider this latter phase by using the Guderley self-similar solution for converging flows. Our model accounts for the fusion reaction energy deposition, thermal and radiation losses thus describing the basic physics of hot spot ignition. The ignition criterion derived from the analytical model is successfully compared with full scale hydrodynamic simulations.

  20. Magnetic quadrupoles lens for hot spot proton imaging in inertial confinement fusion

    Energy Technology Data Exchange (ETDEWEB)

    Teng, J. [Science and Technology on Plasma Physics Laboratory, Laser Fusion Research Center, China Academy of Engineering Physics, Mianyang 621900 (China); Gu, Y.Q., E-mail: yqgu@caep.cn [Science and Technology on Plasma Physics Laboratory, Laser Fusion Research Center, China Academy of Engineering Physics, Mianyang 621900 (China); Center for Applied Physics and Technology, HEDPS, Peking University, Beijing 100871 (China); Chen, J.; Zhu, B.; Zhang, B.; Zhang, T.K.; Tan, F.; Hong, W.; Zhang, B.H. [Science and Technology on Plasma Physics Laboratory, Laser Fusion Research Center, China Academy of Engineering Physics, Mianyang 621900 (China); Wang, X.Q. [Academy of Nuclear Science and Technology, University of Science and Technology of China, Hefei 230026 (China)

    2016-08-01

    Imaging of DD-produced protons from an implosion hot spot region by miniature permanent magnetic quadrupole (PMQ) lens is proposed. Corresponding object-image relation is deduced and an adjust method for this imaging system is discussed. Ideal point-to-point imaging demands a monoenergetic proton source; nevertheless, we proved that the blur of image induced by proton energy spread is a second order effect therefore controllable. A proton imaging system based on miniature PMQ lens is designed for 2.8 MeV DD-protons and the adjust method in case of proton energy shift is proposed. The spatial resolution of this system is better than 10 μm when proton yield is above 10{sup 9} and the spectra width is within 10%.

  1. Thermally smart characteristics of nanofluids in parallel microchannel systems to mitigate hot spots in MEMS

    CERN Document Server

    Maganti, Lakshmi Sirisha; Sundararajan, T; Das, Sarit K

    2016-01-01

    Mitigation of hot spots in MEMS employing in situ microchannel systems requires a comprehensive picture of the maldistribution of the working fluid and uniformity of cooling within the same. In this article, detailed simulations employing parallel micro channel systems with specialized manifold-channel configurations i.e. U, I and Z have been performed. Eulerian Lagrangian Discrete Phase Model and Effective Property Model with water and alumina water nanofluid as working fluids have been employed. The distributions of the dispersed particulate phase and continuous phase have been observed to be, in general, different from the flow distribution and this has been found to be strongly dependent on the flow configuration. Particle maldistribution has been conclusively shown to be influenced by various migration and diffusive phenomena like Stokesian drag, Brownian motion, thermophoretic drift, etc. To understand the uniformity of cooling within the device, which is of importance in real time scenario, an appropri...

  2. Integrative Analysis of Hot Spot Conditions in MagLIF Experiments

    Science.gov (United States)

    Knapp, Patrick; Gomez, Matthew; Harding, Eric; Hansen, Stephanie; Hahn, Kelly; Geissel, Matthias; Chandler, Gordon; Smith, Ian; Slutz, Steve; Jennings, Chris; Martin, Matthew; Schmit, Paul; Peterson, Kyle; Rochau, Gregory; McBride, Ryan; Sinars, Daniel

    2016-10-01

    A large data set incorporating all available neutron and x-ray data is used to analyze a broad range of Magnetized Liner Inertial Fusion (MagLIF) experiments conducted on the Z machine at Sandia National Laboratories over the past two years. Electron and ion temperatures, electron density, mix fraction, burn volume and duration, and neutron and x-ray yields are all measured on each experiment; several through multiple independent methods. Complementary methods are used to infer the hot spot energy and pressure, and trends are analyzed. The results are placed in the context of accepted performance metrics for Magneto-Inertial Fusion. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE.

  3. Simulation of the radiation from the hot spot of an X-pinch

    Science.gov (United States)

    Oreshkin, V. I.; Artyomov, A. P.; Chaikovsky, S. A.; Oreshkin, E. V.; Rousskikh, A. G.

    2017-01-01

    The results of X-pinch experiments performed using a small-sized pulse generator are analyzed. The generator, capable of producing a 200-kA, 180-ns current, was loaded with an X-pinch made of four 35-μm-diameter aluminum wires. The analysis consists of a one-dimensional radiation magnetohydrodynamic simulation of the formation of a hot spot in an X-pinch, taking into account the outflow of material from the neck region. The radiation loss and the ion species composition of the pinch plasma are calculated based on a stationary collisional-radiative model, including balance equations for the populations of individual levels. With this model, good agreement between simulation predictions and experimental data has been achieved: the experimental and the calculated radiation power and pulse duration differ by no more than twofold. It has been shown that the x-ray pulse is formed in the radiative collapse region, near its boundary.

  4. Enhancement of electron hot spot relaxation in photoexcited plasmonic structures by thermal diffusion

    CERN Document Server

    Spitzer, F; Belotelov, V I; Vondran, J; Akimov, I A; Kasture, S; Achanta, V G; Yakovlev, D R; Bayer, M

    2016-01-01

    We demonstrate that in confined plasmonic metal structures subject to ultra-fast laser excitation electron thermal diffusion can provide relaxation faster than the energy transfer to the lattice. This relaxation occurs due to excitation of nanometer-sized hot spots in the confined structure and the sensitivity of its optical parameters to the perturbation in these regions. Both factors become essential when the plasmonic resonance condition is met for both excitation and detection. A pump-probe experiment on plasmonic gold lattices shows sub-picosecond relaxation with the characteristic times well-described by a two-temperature model. The results suggest that dynamical optical response in plasmonic structures can be tuned by selection of the structural geometry as well as the choice of wavelength and polarization of the excitation and detection light.

  5. Is the Juan Fernandez Ridge (nazca Plate) a Deep-Mantle Hot SPOT Trail?

    Science.gov (United States)

    Lara, L. E.; Selles, D.; Díaz, A.; Piña-Gauthier, M.

    2011-12-01

    The Juan Fernández Ridge on the oceanic Nazca plate is thought to be a classic hot spot trail because of the apparent westward rejuvenation of the eruptive ages. Geochronological data is still scarce to prove this is the case, and other hypothesis should be taken into account. There are a few constrains, like the ca. 9 Ma Ar-Ar age of the O'Higgins seamount (115 km from the Chile-Perú trench), published K-Ar ages of ca. 3-4 Ma in Robinson Crusoe island (580 km from the trench) and ca. 1 Ma in Alejandro Selkirk (180 km further west). New reconnaissance K-Ar ages in Robinson Crusoe yield ca. 1-3 Ma, which partially overlap with the age of Alejandro Selkirk, breaking the expected age progression given that the Nazca plate moves eastwards at ca. 6-8 cm/yr. New geological mapping also shows a sharp unconformity between the older, strongly altered sequences and more recent, post-erosional volcanic piles, where only the vent facies have disappeared. A fixed deep-mantle plume origin for Pacific hot spots has been widely debated and concurrent phenomena arose as a possible explanation for non-linear age progressions and/or long-lived volcanic activity. In fact, intraplate regional tectonics, plume displacement, and mantle heterogeneities could be the main factor of the ridge architecture or the mask for a first-order linear trend. An ongoing mapping and dating effort is aimed to understand the evolution of the Juan Fernández Ridge, testing the main hypothesis. Fondecyt grant 110966 is acknowledged for financial support.

  6. Identification of interacting hot spots in the beta3 integrin stalk using comprehensive interface design.

    Science.gov (United States)

    Donald, Jason E; Zhu, Hua; Litvinov, Rustem I; DeGrado, William F; Bennett, Joel S

    2010-12-03

    Protein-protein interfaces are usually large and complementary surfaces, but specific side chains, representing energetic "hot spots," often contribute disproportionately to binding free energy. We used a computational method, comprehensive interface design, to identify hot spots in the interface between the stalk regions of the β3 and the complementary αIIb and αv integrin subunits. Using the Rosetta alanine-scanning and design algorithms to predict destabilizing, stabilizing, and neutral mutations in the β3 region extending from residues Lys(532) through Gly(690), we predicted eight alanine mutations that would destabilize the αIIbβ3 interface as well as nine predicted to destabilize the αvβ3 interface, by at least 0.3 kcal/mol. The mutations were widely and unevenly distributed, with four between residues 552 and 563 and five between 590 and 610, but none between 565 and 589, and 611 and 655. Further, mutations destabilizing the αvβ3 and αIIbβ3 interfaces were not identical. The predictions were then tested by introducing selected mutations into the full-length integrins expressed in Chinese hamster ovary cells. Five mutations predicted to destabilize αIIb and β3 caused fibrinogen binding to αIIbβ3, whereas three of four predicted to be neutral or stabilizing did not. Conversely, a mutation predicted to destabilize αvβ3, but not αIIbβ3 (D552A), caused osteopontin binding to αvβ3, but not fibrinogen binding to αIIbβ3. These results indicate that stability of the distal stalk interface is involved in constraining integrins in stable, inactive conformations. Further, they demonstrate the ability of comprehensive interface design to identify functionally significant integrin mutations.

  7. Analysis on hot spot mutations of GJB2 gene and mitochondrial DNA 12S rRNA among consanguineous induced deafness families%近亲婚配致聋家系中患者GJB2和线粒体DNA 12S rRNA基因突变热点分析

    Institute of Scientific and Technical Information of China (English)

    杨威; 付四清; 董家曙; 王春芳; 陈观明

    2011-01-01

    Objective: To analyze the frequencies of hot spot mutations of the two common deafness gene - GJB2 and mitochondrial DNA 12S rRNA in the consanguineous induced deafness families. Methods: 25 consanguineous induced deafness families received comprehensive physical examination and pure tone test, 48 cases were found, 3 mi venous blood samples were obtained to extract DNA, polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) targeted to specific genes were performed, the cases with mutation were confirmed by gene sequencing. Results: 1 case was found with 235delC homozygous mutation of GJB2 gene, 3 cases were found with heterozygous mutation; no A 1555G mutation was found in mitochondrial DNA 12S rRNA. Conclusion: The most of consanguineous induced deafness are autosomal recessive inheritance, but the incidence of 235delC mutation of GJB2 gene is low.%目的:分析GJB2和线粒体DNA 12S rRNA两种常见耳聋致病基因突变热点在近亲婚配致聋家系患者中的发生频率.方法:对25个近亲结婚致聋核心家系的后代进行全面的体格检查和纯音测试,共发现患者48例,采静脉血3 ml,提取DNA,针对特定基因,进行聚合酶链式反应(Polymerase chain reaction,PCR)及限制性片段长度多态性(Restriction fragmentlength polymorphism,RFLP)分析,发现突变者测序验证.结果:发现GJB2基因235delC纯合突变1例,杂合突变3例;未发现线粒体DNA 12S rRNA A1555G突变.结论:近亲结婚家系中遗传性聋多为常染色体隐性遗传,但GJB2基因235delc突变的发生率较低.

  8. Standard Test Method for Hot Spot Protection Testing of Photovoltaic Modules

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2008-01-01

    1.1 This test method provides a procedure to determine the ability of a photovoltaic (PV) module to endure the long-term effects of periodic “hot spot” heating associated with common fault conditions such as severely cracked or mismatched cells, single-point open circuit failures (for example, interconnect failures), partial (or non-uniform) shadowing or soiling. Such effects typically include solder melting or deterioration of the encapsulation, but in severe cases could progress to combustion of the PV module and surrounding materials. 1.2 There are two ways that cells can cause a hot spot problem; either by having a high resistance so that there is a large resistance in the circuit, or by having a low resistance area (shunt) such that there is a high-current flow in a localized region. This test method selects cells of both types to be stressed. 1.3 This test method does not establish pass or fail levels. The determination of acceptable or unacceptable results is beyond the scope of this test method....

  9. Study on the resistance spot welding technology of 22MnMoB hot stamping quenched steel

    Institute of Scientific and Technical Information of China (English)

    Feng Yi; Ma Mingtu; Hua Fuan; Zhang Junping; Song Leifeng; Jin Qingsheng

    2014-01-01

    In this paper,the spot welding technology of a new kind of 22MnMoB hot stamping quenched steel sheet was systematically studied by power frequency spot welder. Through a series of technology and test exper-iments,we have obtained the optimal spot welding technological parameter condition. According to the results, the relations among spot welding technological parameter,welding nugget,mechanical property and fracture mode were discussed. The effects of all the welding parameters such as welding current,welding time and elec-trode force on the quality of joint can be boiled down to one thing-the diameter of welding nugget. The experi-mental results showed that welding nugget diameter determines the mechanical property of spot welding joint and the relation between welding nugget diameter and the mechanical property of joint presents a kind of linear mathematic representation. There are two typical fracture models of 22MnMoB hot stamping quenched steel sheet,i.e.,interfacial fracture and nugget pullout. Other than mild steel or normal high strength steel,in the shearing tensile test,hot stamping quenched steel has a great tendency to fail in interfacial mode due to the ef-fects of high strength matrix structure,welding soft zone and the porosity level of fusion zone.

  10. Measurement of the hot spot electron temperature in NIF ICF implosions using Krypton x-ray emission spectroscopy

    Science.gov (United States)

    Ma, T.; Chen, H.; Patel, P. K.; Schneider, M.; Barrios, M.; Berzak Hopkins, L.; Casey, D.; Chung, H.-K.; Hammel, B.; Jarrott, C.; Nora, R.; Pak, A.; Scott, H.; Spears, B.; Weber, C.

    2015-11-01

    The inference of ion temperature from neutron spectral measurements in indirect-drive ICF implosions is known to be sensitive to non-thermal velocity distributions in the fuel. The electron temperature (Te) inferred from dopant line ratios should not be sensitive to these bulk motions and hence may be a better measure of the thermal temperature of the hot spot. Here we describe a series of experiments to be conducted on the NIF where a small concentration of a mid-Z dopant (Krypton) is added to the fuel gas. The x-ray spectra is measured and the electron temperature is inferred from Kr line ratios. We also quantify the level of radiative cooling in the hot spot due to this mid-Z dopant. These experiments represent the first direct measurement of hot spot Te using spectroscopy, and we will describe the considerations for applying x-ray spectroscopy in such dense and non-uniform hot spots. This work performed under the auspices of U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

  11. Localization of Temperature Using Plasmonic Hot Spots in Metal Nanostructures: The Nano-Optical Antenna Approach and Fano Effect

    CERN Document Server

    Khorashad, Larousse Khosravi; Valentine, Jason; Govorov, Alexander O

    2016-01-01

    It is challenging to strongly localize temperature in small volumes because heat transfer is a diffusive process. Here we show how to overcome this limitation using electrodynamic hot spots and interference effects in the regime of continuous-wave (CW) excitation. We introduce a set of figures of merit for the localization of temperature and for the efficiency of the plasmonic photo-thermal effect. Our calculations show that the temperature localization in a trimer nanoparticle assembly is a complex function of the geometry and sizes. Large nanoparticles in the trimer play the role of the nano-optical antenna whereas the small nanoparticle in the plasmonic hot spot acts as a nano-heater. Under the peculiar conditions, the temperature increase inside a nanoparticle trimer can be localized in a hot spot region at the small heater nanoparticle and, in this way, a thermal hot spot can be realized. However, the overall power efficiency of temperature generation in this trimer is much smaller than that of a single ...

  12. 75 FR 29537 - Draft Transportation Conformity Guidance for Quantitative Hot-spot Analyses in PM2.5

    Science.gov (United States)

    2010-05-26

    ... [Federal Register Volume 75, Number 101 (Wednesday, May 26, 2010)] [Notices] [Pages 29537-29538... Transportation Conformity Guidance for Quantitative Hot- spot Analyses in PM 2.5 and PM 10 Nonattainment and... Public Comment. SUMMARY: EPA is announcing the availability of a draft transportation conformity guidance...

  13. Tree Coring as a Complement to Soil Gas Screening to Locate PCE and TCE Source Zones and Hot Spots

    DEFF Research Database (Denmark)

    Nielsen, Mette Algreen; Trapp, Stefan; Rehne Jensen, Pernille

    2015-01-01

    ) or trichloroethylene (TCE) to evaluate their ability to locate source zones and contaminant hot spots. One test site represented a relatively homogeneous sandy soil and aquifer, and the second a more heterogeneous geology with both sandy and less permeable clay till layers overlying a chalk aquifer. Tree cores from...

  14. Evaluation of the influence of bypass flow gap distribution on the core hot spot in a prismatic VHTR core

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Min-Hwan, E-mail: mhkim@kaeri.re.kr [Korea Atomic Energy Research Institute, 1045 Daedeokdaero, Yoseong, Daejeon 304-353 (Korea, Republic of); Lim, Hong-Sik [Korea Atomic Energy Research Institute, 1045 Daedeokdaero, Yoseong, Daejeon 304-353 (Korea, Republic of)

    2011-08-15

    Highlights: > A procedure to evaluate the local gap size variation between graphite blocks was developed and applied to a prismatic core VHTR. > The analysis for the core bypass flow and hot spot was carried out based on the calculated gap distributions. > The predicted gap size is large enough to affect the flow distribution in the core. > The bypass gap and flow distributions are closely related to the local hot spot temperature and its location. > The core restraint mechanism preventing outward movement of graphite block reduces the bypass gap size and hot spot temperature. - Abstract: Core bypass flow in VHTR is one of the key issues for core thermal margins and efficiency. The bypass flow in the prismatic core varies during core cycles due to the irradiation shrinkage/swelling and thermal expansion of the graphite blocks. A procedure to evaluate the local gap size variation between graphite blocks was developed and applied to a prismatic core VHTR. The influence of the core restraint mechanism on the bypass flow gap was evaluated. The predicted gap size is as much as 8 mm when the graphite block is exposed to its allowable limit of fast neutron fluence. The analysis for the core bypass flow and hot spot was carried out based on the calculated gap distributions. The results indicate that the bypass gap and flow distributions are closely related to the local hot spot and its location and the core restraint mechanism preventing outward movement of the graphite block by a fastening device reduces the bypass gap size, which results in the decrease of maximum fuel temperature not less than 100 deg. C, when compared to the case without it.

  15. Effects of high shock pressures and pore morphology on hot spot mechanisms in HMX

    Science.gov (United States)

    Springer, H. K.; Tarver, C. M.; Bastea, S.

    2017-01-01

    The shock initiation and detonation behavior of heterogeneous solid explosives is governed by its microstructure and reactive properties. New additive manufacturing techniques offer unprecedented control of explosive microstructures previously impossible, enabling us to develop novel explosives with tailored shock sensitivity and detonation properties. Since microstructure-performance relationships are not well established for explosives, there is little material design guidance for these manufacturing techniques. In this study, we explore the effects of high shock pressures (15-38 GPa) with long shock durations and different pore morphologies on hot spot mechanisms in HMX. HMX is chosen as the model material because we have experimental data on many of the chemical-thermal-mechanical properties required for pore collapse simulations. Our simulations are performed using the multi-physics arbitrary Lagrangian Eulerian finite element hydrocode, ALE3D, with Cheetah-based models for the unreacted and the product equation-of-states. We use a temperature-dependent specific heat with the unreacted equation-of-state and a temperature-dependent viscosity model to ensure accurate shock temperatures for subsequent chemistry. The Lindemann Law model is used for shock melting in HMX. In contrast to previous pore collapse studies at lower shock pressures (≤10 GPa) in HMX and shorter post-collapse burning times, our calculations show that shock melting occurs above 15 GPa due to higher bulk heating and a prominent elongated ("jet-like") hot spot region forms at later times. The combination of the elongated, post-collapse hot spot region and the higher bulk heating with increasing pressure dramatically increases the growth rate of reaction. Our calculations show that the reaction rate, dF/dt, increases with increasing shock pressure. We decompose the reaction rate into ignition ((dF/dt)ig) and growth ((dF/dt)gr) phases to better analyze our results. We define the ignition phase

  16. Spin-up and hot spots can drive mass out of a binary

    Science.gov (United States)

    van Rensbergen, W.; De Greve, J. P.; De Loore, C.; Mennekens, N.

    2008-09-01

    Context: The observed distribution of orbital periods of Algols with a B-type primary at birth agrees fairly well with the prediction from conservative theory. Conservative evolution fails, however, to produce the rather large fraction of Algols observed with a high mass-ratio, especially: q in [0.4-0.6]. Aims: In order to keep Algols for a longer time with a higher mass-ratio without disturbing the distribution of orbital periods too much, interacting binaries have to lose a significant fraction of their total mass without losing much angular momentum before or during Algolism. We propose a mechanism that meets both requirements. Methods: In the case of direct impact the gainer spins up: sometimes up to critical velocity. Equatorial material on the gainer is therefore less bound. A similar statement applies to material located at the edge of an accretion disc. The incoming material moreover creates a hot spot in the area of impact. The sum of the rotational and radiative energy of hot spot material depends on the mass-transfer-rate. The sum of both energies overcomes the binding energy at a well defined critical value of the mass-transfer-rate. As long as the transfer-rate is smaller than this critical value RLOF happens conservatively. But as soon as the critical rate is exceeded the gainer will acquire no more than the critical value and RLOF runs into a liberal era. Results: Low-mass binaries never achieve mass-transfer-rates larger than the critical value. Intermediate-mass binaries evolve mainly conservatively but mass will be blown away from the system during the short era of rapid mass-transfer soon after the onset of RLOF. We have calculated the evolution of binaries with a 9 M⊙ primary and a 5.4 M⊙ companion over a range of initial orbital periods, covering case-A RLOF. Mass-loss from the system is achieved during direct impact only. Conclusions: We find systems that show Algolism for more than ten million years. RLOF occurs almost always

  17. Prediction of hot spot residues at protein-protein interfaces by combining machine learning and energy-based methods

    Directory of Open Access Journals (Sweden)

    Pontil Massimiliano

    2009-10-01

    Full Text Available Abstract Background Alanine scanning mutagenesis is a powerful experimental methodology for investigating the structural and energetic characteristics of protein complexes. Individual amino-acids are systematically mutated to alanine and changes in free energy of binding (ΔΔG measured. Several experiments have shown that protein-protein interactions are critically dependent on just a few residues ("hot spots" at the interface. Hot spots make a dominant contribution to the free energy of binding and if mutated they can disrupt the interaction. As mutagenesis studies require significant experimental efforts, there is a need for accurate and reliable computational methods. Such methods would also add to our understanding of the determinants of affinity and specificity in protein-protein recognition. Results We present a novel computational strategy to identify hot spot residues, given the structure of a complex. We consider the basic energetic terms that contribute to hot spot interactions, i.e. van der Waals potentials, solvation energy, hydrogen bonds and Coulomb electrostatics. We treat them as input features and use machine learning algorithms such as Support Vector Machines and Gaussian Processes to optimally combine and integrate them, based on a set of training examples of alanine mutations. We show that our approach is effective in predicting hot spots and it compares favourably to other available methods. In particular we find the best performances using Transductive Support Vector Machines, a semi-supervised learning scheme. When hot spots are defined as those residues for which ΔΔG ≥ 2 kcal/mol, our method achieves a precision and a recall respectively of 56% and 65%. Conclusion We have developed an hybrid scheme in which energy terms are used as input features of machine learning models. This strategy combines the strengths of machine learning and energy-based methods. Although so far these two types of approaches have mainly been

  18. Optical and mechanical manipulation of plasmonic hot spots on gold nanoparticle tips; Optische und mechanische Manipulation plasmonischer Heisspunkte an Goldnanopartikelspitzen

    Energy Technology Data Exchange (ETDEWEB)

    Hrelescu, Calin-Alexander

    2011-06-15

    in the present thesis hybride systems based on alternative nanoparticle morphologies were studied in view of their optical properties in order to remove extensively the known limitations and insufficiencies of the established nanoparticle systems and shapes like rod-shaped or spherical nanoparticles. Star-shaped gold nanoparticles and their special plasmonic properties are presented. By methods of the dark-field spectroscopy, the photoelectron emission spectroscopy, and scanning electron microscopy the near- and far-field properties of single nanostars and their morphologies are correlated. So the first experimental proof of the localization of optically excited hot spots on the tips of the single nanostars could be given. By suited choice of the polarization and wavelength hot spots on several or exclusively single tips of a nanostar are selectively excited and the detected plasmon resonances assigned to single nanostar tips only due to experimental data. Furthermore field amplification factors in the range of 58-79 are for the first time quantitatively determined. The high and for molecules easily accessible hot spots on the tips of the nanostars are applied in order to demonstrate the excellent amplification of the Raman scattering on the level of single nanostars. The Raman amplification factors of 10{sup 7} for the studied nanostars without use of plasmonic coupling effects mark the nanostars as important candidates for the application in complex dynamical environments. A further possibility of the use of the hot spots localized on tips offer nanopyramides. By manipulation with a scanning force microscope a positioning of the hot spots until 1 nm above the surface and on arbitrary places on a sample substrate can be reached. Nanoresonators of two nanopyramides can be mechanically fabricated in three adsorption configurations concerning the tips of the two nanoparticles directed to each other. By the mechanical change of the configuration of the nanoresonator

  19. A Machine Learning Approach for Hot-Spot Detection at Protein-Protein Interfaces

    Directory of Open Access Journals (Sweden)

    Rita Melo

    2016-07-01

    Full Text Available Understanding protein-protein interactions is a key challenge in biochemistry. In this work, we describe a more accurate methodology to predict Hot-Spots (HS in protein-protein interfaces from their native complex structure compared to previous published Machine Learning (ML techniques. Our model is trained on a large number of complexes and on a significantly larger number of different structural- and evolutionary sequence-based features. In particular, we added interface size, type of interaction between residues at the interface of the complex, number of different types of residues at the interface and the Position-Specific Scoring Matrix (PSSM, for a total of 79 features. We used twenty-seven algorithms from a simple linear-based function to support-vector machine models with different cost functions. The best model was achieved by the use of the conditional inference random forest (c-forest algorithm with a dataset pre-processed by the normalization of features and with up-sampling of the minor class. The method has an overall accuracy of 0.80, an F1-score of 0.73, a sensitivity of 0.76 and a specificity of 0.82 for the independent test set.

  20. Direct simulations of homogeneous bubble nucleation: Agreement with classical nucleation theory and no local hot spots.

    Science.gov (United States)

    Diemand, Jürg; Angélil, Raymond; Tanaka, Kyoko K; Tanaka, Hidekazu

    2014-11-01

    We present results from direct, large-scale molecular dynamics simulations of homogeneous bubble (liquid-to-vapor) nucleation. The simulations contain half a billion Lennard-Jones atoms and cover up to 56 million time steps. The unprecedented size of the simulated volumes allows us to resolve the nucleation and growth of many bubbles per run in simple direct micro-canonical simulations while the ambient pressure and temperature remain almost perfectly constant. We find bubble nucleation rates which are lower than in most of the previous, smaller simulations. It is widely believed that classical nucleation theory (CNT) generally underestimates bubble nucleation rates by very large factors. However, our measured rates are within two orders of magnitude of CNT predictions; only at very low temperatures does CNT underestimate the nucleation rate significantly. Introducing a small, positive Tolman length leads to very good agreement at all temperatures, as found in our recent vapor-to-liquid nucleation simulations. The critical bubbles sizes derived with the nucleation theorem agree well with the CNT predictions at all temperatures. Local hot spots reported in the literature are not seen: Regions where a bubble nucleation event will occur are not above the average temperature, and no correlation of temperature fluctuations with subsequent bubble formation is seen.

  1. Hot spot model of MagLIF implosions: Nernst term effect on magnetic flux losses

    Science.gov (United States)

    Garcia Rubio, Fernando; Sanz Recio, Javier; Betti, Riccardo

    2016-10-01

    An analytical model of a collisional plasma being compressed by a cylindrical liner is proposed and solved in a magnetized liner inertial fusion-like context. The implosion is assumed to be isobaric, and the magnetic diffusion is confined to a thin layer near the liner. Both unmagnetized and magnetized plasma cases are considered. The model reduces to a system of two partial differential equations for temperature and magnetic field. Special attention is given to the effect of the Nernst term on the evolution of the magnetic field. Scaling laws for temperature, magnetic field, hot spot mass increase and magnetic field losses are obtained. The temperature and magnetic field spatial profiles tend to a self-similar state. It is found that when the Nernst term is taken into account, the magnetic field is advected towards the liner, and the magnetic flux losses are independent of the magnetic Lewis number. Research supported by the Spanish Ministerio de Economía y Competitividad, Project No. ENE2014-54960R. Acknowledgements to the Laboratory of Laser Energetics (Rochester) for its hospitality.

  2. WLAN Hot Spot services for the automotive and oil industries. A business analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pau, F. [Rotterdam School of Management, Erasmus Research Institute of Management ERIM, Rotterdam (Netherlands); Oremus, M.H.P. [Rolls Royce (BMW) Germany, Dahlewitz (Germany)

    2003-04-01

    While you refuel for gas, why not refuel for information or download vehicle data? This paper analyzes in extensive detail the user segmentation by vehicle usage, service offering, and full business models from WLAN (Wireless Local Area Network) hot spot services delivered to vehicles (private, professional, public) around gas stations. Also analyzed are the parties which play a role in such service authorization, provisioning and delivery, with all the dependencies modelled by attributed digraphs. Service planning is included as to WLAN base station capabilities. Five year financial models (CAPEX,OPEX), and data pertain to two possible service suppliers: multi-service oil companies, and mobile service operators (or MVNO). Model optimization on the return-on-investment (ROI) is carried out for different deployment scenarios, geographical coverage assumptions, as well as tariff structures. Comparison is also being made with public GPRS data services, as precursors for 3G services, and the effect of WLAN roaming is analyzed. Analysis shows that due to manpower costs and marketing costs, suitable ROI will not be achieved unless externalities are accounted for and innovative tariff structures are introduced. Open issues and further research are outlined. Further work is carried out, also with automotive electronics sector, wireless systems providers, wireless terminals platform suppliers, and vehicle manufacturers.

  3. Radio lobes and X-ray hot spots in the microquasar S26

    CERN Document Server

    Soria, Roberto; Broderick, Jess W; Corbel, Stephane; Motch, Christian

    2010-01-01

    We have studied the structure and energetics of the powerful microquasar/shock-ionized nebula S26 in NGC 7793, with particular focus on its radio and X-ray properties. Using the Australia Telescope Compact Array, we have resolved for the first time the radio lobe structure and mapped the spectral index of the radio cocoon. The steep spectral index of the radio lobes is consistent with optically-thin synchrotron emission; outside the lobes, the spectral index is flatter, suggesting an additional contribution from free-free emission, and perhaps ongoing ejections near the core. The radio core is not detected, while the X-ray core has a 0.3-8 keV luminosity ~6 x 10^{36} erg/s. The size of the radio cocoon matches that seen in the optical emission lines and diffuse soft X-ray emission. The total 5.5-GHz flux of cocoon and lobes is ~2.1 mJy, which at the assumed distance of 3.9 Mpc corresponds to about 3 times the luminosity of Cas A. The total 9.0-GHz flux is ~1.6 mJy. The X-ray hot spots (combined 0.3-8 keV lumi...

  4. Simulated impact of self-generated magnetic fields in the hot-spot of NIF implosions

    Science.gov (United States)

    Partha, M. A.; Haan, S. W.; Koning, J.; Marinak, M. M.; Weber, C. R.; Clark, D. S.

    2016-10-01

    Deviations from sphericity in an imploded hot-spot result in magnetic fields generated by the Biermann battery effect. The magnetic field can reduce thermal conductivity, affect α transport, change instability growth, and cause magnetic pressure. Previous estimates of these effects have indicated that they are not of great consequence, but have suggested that they could plausibly affect NIF observables such as yield and ion temperature by 5-25%. Using the MHD capability in the Hydra code, we evaluated the impact of these processes in a post-shot model for a typical NIF implosion. Various implosion asymmetries were implemented, with the goal of surveying plausible implosion configurations to find the geometry in which the MHD effects were the most significant. Magnetic fields are estimated to approach 104 Tesla, and to affect conductivity locally by more than 50%, but global impact on observables is small in most cases. Work performed under the auspices of the U.S. D.O.E. by Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344.

  5. Mutation Hot Spots in Yeast Caused by Long-Range Clustering of Homopolymeric Sequences

    Directory of Open Access Journals (Sweden)

    Xin Ma

    2012-01-01

    Full Text Available Evolutionary theory assumes that mutations occur randomly in the genome; however, studies performed in a variety of organisms indicate the existence of context-dependent mutation biases. Sources of mutagenesis variation across large genomic contexts (e.g., hundreds of bases have not been identified. Here, we use high-coverage whole-genome sequencing of a conditional mismatch repair mutant line of diploid yeast to identify mutations that accumulated after 160 generations of growth. The vast majority of the mutations accumulated as insertion/deletions (in/dels in homopolymeric [poly(dA:dT] and repetitive DNA tracts. Surprisingly, the likelihood of an in/del mutation in a given poly(dA:dT tract is increased by the presence of nearby poly(dA:dT tracts in up to a 1,000 bp region centered on the given tract. Our work suggests that specific mutation hot spots can contribute disproportionately to the genetic variation that is introduced into populations and provides long-range genomic sequence context that contributes to mutagenesis.

  6. Hot spot detection and spatio-temporal dynamics of dengue in Queensland, Australia

    Science.gov (United States)

    Naish, S.; Tong, S.

    2014-11-01

    Dengue has been a major public health concern in Australia since it re-emerged in Queensland in 1992-1993. This study explored spatio-temporal distribution and clustering of locally-acquired dengue cases in Queensland State, Australia and identified target areas for effective interventions. A computerised locally-acquired dengue case dataset was collected from Queensland Health for Queensland from 1993 to 2012. Descriptive spatial and temporal analyses were conducted using geographic information system tools and geostatistical techniques. Dengue hot spots were detected using SatScan method. Descriptive spatial analysis showed that a total of 2,398 locally-acquired dengue cases were recorded in central and northern regions of tropical Queensland. A seasonal pattern was observed with most of the cases occurring in autumn. Spatial and temporal variation of dengue cases was observed in the geographic areas affected by dengue over time. Tropical areas are potential high-risk areas for mosquito-borne diseases such as dengue. This study demonstrated that the locally-acquired dengue cases have exhibited a spatial and temporal variation over the past twenty years in tropical Queensland, Australia. There is a clear evidence for the existence of statistically significant clusters of dengue and these clusters varied over time. These findings enabled us to detect and target dengue clusters suggesting that the use of geospatial information can assist the health authority in planning dengue control activities and it would allow for better design and implementation of dengue management programs.

  7. Suppressing local hot spots due to eddy currents in magnetic coil systems

    Science.gov (United States)

    Yao, Zhen; Shojinaga, Aaron; Wu, Yong; Shvartsman, Shmaryu; Eagan, Timothy; Chmielewski, Thomas; Brown, Robert

    2011-03-01

    A particular goal in magnetic field applications is to avoid eddy current heating in coils and shields. It is important, in MRI, for example, to avoid hot spots near the patient to be imaged as well as in the vicinity of soldering joints. We develop effective analytical formulas for the eddy current behavior of sources close to surrounding conductors, we verify these via numerical simulations, and we make successful comparisons to corresponding experimental temperature distributions. Optimized patterns of incisions made in the conductors are discovered for addressing particularly troublesome heating locations. The criteria include the need to minimize the number and length of the cuts. Theory and experiment are in agreement on the efficacy of this method for reducing steady-state temperatures. An example of results in the practical design of commercial coils and shields is that a single cut parallel to the long edge of rectangular conductors reduces the temperatures much more than making multiple cuts parallel to the short edge. Supported by Ohio Third Frontier Program

  8. New Caledonia: A 'Hot Spot' for Valuable Chemodiversity. Part 1: Gymnosperms.

    Science.gov (United States)

    Coulerie, Paul; Poullain, Cyril

    2015-06-01

    The flora of New Caledonia encompasses more than 3,000 species and almost 80% of them are endemic. New Caledonia is considered as a 'hot spot' for biodiversity. With the current global loss of biodiversity, and the fact that several drugs and pesticides are becoming obsolete, there is an urgent need to increase sampling and research on new natural products. In this context, we review the chemical information available on New Caledonian native flora from economical perspectives. We expect that a better knowledge of the economic potential will encourage the plantation of native plants for the development of a sustainable economy which will participate in the conservation of biodiversity. In the first part of this review, we discuss the results reported in 18 scientific articles on the chemicals isolated from 23 endemic conifers of New Caledonia. Several bioactive and original products, such as neocallitropsene or libocedrins, have been isolated from these conifers. This review also highlights several groups, such as Podocarpus spp., that are unexplored in New Caledonia despite the fact that they have been described in other countries to contain a wide range of original bioactive compounds. Those plants are considered as priority for future chemical investigations.

  9. New Caledonia: A Hot Spot for Valuable Chemodiversity Part 3: Santalales, Caryophyllales, and Asterids.

    Science.gov (United States)

    Coulerie, Paul; Poullain, Cyril

    2016-04-01

    The flora of New Caledonia encompasses more than 3000 plant species and an endemism of almost 80%. New Caledonia is even considered as one of the 34 'hot spots' for biodiversity. Considering the current global loss of biodiversity and the fact that several drugs and pesticides become obsolete, there is an urgent need to increase sampling and research on new natural products. In this context, here, we reviewed the chemical knowledge available on New Caledonian native flora from economical perspectives. We expect that a better knowledge of the economic potential of plant chemistry will encourage the plantation of native plants for the development of a sustainable economy which will participate in the conservation of biodiversity. This review is divided into three parts, and the third part which is presented here summarizes the scientific literature related to the chemistry of endemic santalales, caryophyllales, and asterids. We show that the high rate of endemism is correlated with the originality of phytochemicals encountered in New Caledonian plants. A total of 176 original natural compounds have been identified from these plants, whereas many species have not been investigated so far. We also discuss the economic potential of plants and molecules with consideration of their medicinal and industrial perspectives. This review finally highlights several groups, such as Sapotaceae, that are unexplored in New Caledonia despite the high chemical interest in them. These plants are considered to have priority in future chemical investigations.

  10. Theoretical evaluation of a method for locating gaseous emission hot spots.

    Science.gov (United States)

    Hashmonay, Ram A

    2008-08-01

    This paper describes and theoretically evaluates a recently developed method that provides a unique methodology for mapping gaseous emissions from non-point pollutant sources. The horizontal radial plume mapping (HRPM) methodology uses an open-path, path-integrated optical remote sensing (PI-ORS) system in a horizontal plane to directly identify emission hot spots. The radial plume mapping methodology has been well developed, evaluated, and demonstrated. In this paper, the theoretical basis of the HRPM method is explained in the context of the method's reliability and robustness to reconstruct spatially resolved plume maps. Calculation of the condition number of the inversion's kernel matrix showed that this method has minimal error magnification (EM) when the beam geometry is optimized. Minimizing the condition number provides a tool for such optimization of the beam geometry because it indicates minimized EM. Using methane concentration data collected from a landfill with a tunable diode laser absorption spectroscopy (TDLAS) system, it is demonstrated that EM is minimal because the averaged plume map of many reconstructed plume maps is very similar to a plume map generated by the averaged concentration data. It is also shown in the analysis of this dataset that the reconstructions of plume maps are unique for the optimized HRPM beam geometry and independent of the actual algorithm applied.

  11. A Machine Learning Approach for Hot-Spot Detection at Protein-Protein Interfaces

    Science.gov (United States)

    Melo, Rita; Fieldhouse, Robert; Melo, André; Correia, João D. G.; Cordeiro, Maria Natália D. S.; Gümüş, Zeynep H.; Costa, Joaquim; Bonvin, Alexandre M. J. J.; Moreira, Irina S.

    2016-01-01

    Understanding protein-protein interactions is a key challenge in biochemistry. In this work, we describe a more accurate methodology to predict Hot-Spots (HS) in protein-protein interfaces from their native complex structure compared to previous published Machine Learning (ML) techniques. Our model is trained on a large number of complexes and on a significantly larger number of different structural- and evolutionary sequence-based features. In particular, we added interface size, type of interaction between residues at the interface of the complex, number of different types of residues at the interface and the Position-Specific Scoring Matrix (PSSM), for a total of 79 features. We used twenty-seven algorithms from a simple linear-based function to support-vector machine models with different cost functions. The best model was achieved by the use of the conditional inference random forest (c-forest) algorithm with a dataset pre-processed by the normalization of features and with up-sampling of the minor class. The method has an overall accuracy of 0.80, an F1-score of 0.73, a sensitivity of 0.76 and a specificity of 0.82 for the independent test set. PMID:27472327

  12. HOT AND COLD SPOT COUNTS AS PROBES OF NON-GAUSSIANITY IN THE COSMIC MICROWAVE BACKGROUND

    Energy Technology Data Exchange (ETDEWEB)

    Chingangbam, Pravabati [Indian Institute of Astrophysics, Koramangala II Block, Bangalore 560034 (India); Park, Changbom [Korea Institute for Advanced Study, 85 Hoegiro, Dongdaemun-gu, Seoul 130-722 (Korea, Republic of); Yogendran, K. P. [Indian Institute for Science Education and Research, Mohali (India); Van de Weygaert, Rien, E-mail: prava@iiap.res.in, E-mail: cbp@kias.re.kr, E-mail: pattag@gmail.com, E-mail: weygaert@astro.rug.nl [Kapteyn Astronomical Institute, University of Groningen, P.O. Box 800, 9747 AV Groningen (Netherlands)

    2012-08-20

    We introduce the numbers of hot and cold spots, n{sub h} and n{sub c} , of excursion sets of the cosmic microwave background (CMB) temperature anisotropy maps as statistical observables that can discriminate different non-Gaussian models. We numerically compute them from simulations of non-Gaussian CMB temperature fluctuation maps. The first kind of non-Gaussian model we study is the local type primordial non-Gaussianity. The second kind of model has some specific form of the probability distribution function from which the temperature fluctuation value at each pixel is drawn, obtained using HEALPIX. We find the characteristic non-Gaussian deviation shapes of n{sub h} and n{sub c} , which is distinct for each of the models under consideration. We further demonstrate that n{sub h} and n{sub c} carry additional information compared to the genus, which is just their linear combination, making them valuable additions to the Minkowski Functionals in constraining non-Gaussianity.

  13. Emerging Hot Spot Analysis of Tracking Data from a Logistics Company

    DEFF Research Database (Denmark)

    Reinau, Kristian Hegner

    2017-01-01

    Freight transport has received increasing attention in recent years as regions and cities across Europe are struggling with congestion issues while the industry demands more efficient freight transport. Consequently, there is a need for knowledge about how fast freight moves on the roads and sign......Freight transport has received increasing attention in recent years as regions and cities across Europe are struggling with congestion issues while the industry demands more efficient freight transport. Consequently, there is a need for knowledge about how fast freight moves on the roads...... and significantly about the location of the bottlenecks. This paper analyses whether such knowledge can be generated using tracking data from logistics companies. The chosen case region is North Jutland in Denmark, and the method deployed is emerging hot spot analysis on tracking data from approximately 1......,500 trailers provided by one large Danish logistics company for the period 2012-2016. This methodology is relatively new in the transport field and has never been used in a Danish context before, and the paper shows that by using the tracking data and this methodology it is possible to identify where freight...

  14. The Effect of Particle Properties on Hot Particle Spot Fire Ignition

    Science.gov (United States)

    Zak, Casey David

    The ignition of natural combustible material by hot metal particles is an important fire ignition pathway by which wildland and wildland-urban-interface spot fires are started. There are numerous cases reported of wild fires started by clashing power-lines or from sparks generated by machines or engines. Similarly there are many cases reported of fires caused by grinding, welding and cutting sparks. Up to this point, research on hot particle spot fire ignition has largely focused on particle generation and transport. A small number of studies have examined what occurs after a hot particle contacts a natural fuel bed, but until recently the process remained poorly understood. This work describes an investigation of the effect of particle size, temperature and thermal properties on the ability of hot particles to cause flaming ignition of cellulosic fuel beds. Both experimental and theoretical approaches are used, with a focus on understanding the physics underlying the ignition process. For the experimental study, spheres of stainless steel, aluminum, brass and copper are heated in a tube furnace and dropped onto a powdered cellulose fuel bed; the occurrence of flaming ignition or lack thereof is visually observed and recorded. This procedure is repeated a large number of times for each metal type, varying particle diameter from 2 to 11 mm and particle temperature between 575 and 1100°C. The results of these experiments are statistically analyzed to find approximate ignition boundaries and identify boundary trends with respect to the particle parameters of interest. Schlieren images recorded during the ignition experiments are also used to more accurately describe the ignition process. Based on these images, a simple theoretical model of hot particle spot fire ignition is developed and used to explore the experimental trends further. The model under-predicts the minimum ignition temperatures required for small spheres, but agrees qualitatively with the experimental

  15. “Rings of saturn-like” nanoarrays with high number density of hot spots for surface-enhanced Raman scattering

    Energy Technology Data Exchange (ETDEWEB)

    Dai, Zhigao; Liao, Lei; Wu, Wei; Guo, Shishang; Zhao, Xinyue; Li, Wei; Ren, Feng; Jiang, Changzhong, E-mail: xxh@whu.edu.cn, E-mail: czjiang@whu.edu.cn [Department of Physics, Hubei Nuclear Solid Physics Key Laboratory and Center for Ion Beam Application, Wuhan University, Wuhan 430072 (China); Mei, Fei [Department of Physics, Hubei Nuclear Solid Physics Key Laboratory and Center for Ion Beam Application, Wuhan University, Wuhan 430072 (China); School of Electrical and Electronic Engineering, Hubei University of Technology, Wuhan 430068 (China); Xiao, Xiangheng, E-mail: xxh@whu.edu.cn, E-mail: czjiang@whu.edu.cn [Department of Physics, Hubei Nuclear Solid Physics Key Laboratory and Center for Ion Beam Application, Wuhan University, Wuhan 430072 (China); Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095 (United States); Fu, Lei; Wang, Jiao [College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072 (China)

    2014-07-21

    The Ag nanoparticles (NPs) surrounding triangular nanoarrays (TNAs) with high number density of surface-enhanced Raman scattering (SERS) hot spots (SERS hot spots ring) are prepared by a combination of NPs deposition and subsequent colloid lithography processing. Owing to the SERS hot spots ring, the Ag NPs surrounding TNAs have been proved an excellent candidate for ultrasensitive molecular sensing for their high SERS signal enhancing capacity in experiments and theories. The Ag NPs surrounding TNAs can be readily used for the quick detection of low concentrations of molecules related to food safety; herein, detection of melamine is discussed.

  16. The role of hot spot mix in the low-foot and high-foot implosions on the NIF

    Science.gov (United States)

    Ma, T.; Patel, P. K.; Izumi, N.; Springer, P. T.; Key, M. H.; Atherton, L. J.; Barrios, M. A.; Benedetti, L. R.; Bionta, R.; Bond, E.; Bradley, D. K.; Caggiano, J.; Callahan, D. A.; Casey, D. T.; Celliers, P. M.; Cerjan, C. J.; Church, J. A.; Clark, D. S.; Dewald, E. L.; Dittrich, T. R.; Dixit, S. N.; Döppner, T.; Dylla-Spears, R.; Edgell, D. H.; Epstein, R.; Field, J.; Fittinghoff, D. N.; Frenje, J. A.; Gatu Johnson, M.; Glenn, S.; Glenzer, S. H.; Grim, G.; Guler, N.; Haan, S. W.; Hammel, B. A.; Hatarik, R.; Herrmann, H. W.; Hicks, D.; Hinkel, D. E.; Berzak Hopkins, L. F.; Hsing, W. W.; Hurricane, O. A.; Jones, O. S.; Kauffman, R.; Khan, S. F.; Kilkenny, J. D.; Kline, J. L.; Kozioziemski, B.; Kritcher, A.; Kyrala, G. A.; Landen, O. L.; Lindl, J. D.; Le Pape, S.; MacGowan, B. J.; Mackinnon, A. J.; MacPhee, A. G.; Meezan, N. B.; Merrill, F. E.; Moody, J. D.; Moses, E. I.; Nagel, S. R.; Nikroo, A.; Pak, A.; Parham, T.; Park, H.-S.; Ralph, J. E.; Regan, S. P.; Remington, B. A.; Robey, H. F.; Rosen, M. D.; Rygg, J. R.; Ross, J. S.; Salmonson, J. D.; Sater, J.; Sayre, D.; Schneider, M. B.; Shaughnessy, D.; Sio, H.; Spears, B. K.; Smalyuk, V.; Suter, L. J.; Tommasini, R.; Town, R. P. J.; Volegov, P. L.; Wan, A.; Weber, S. V.; Widmann, K.; Wilde, C. H.; Yeamans, C.; Edwards, M. J.

    2017-05-01

    Hydrodynamic mix of the ablator into the DT fuel layer and hot spot can be a critical performance limitation in inertial confinement fusion implosions. This mix results in increased radiation loss, cooling of the hot spot, and reduced neutron yield. To quantify the level of mix, we have developed a simple model that infers the level of contamination using the ratio of the measured x-ray emission to the neutron yield. The principal source for the performance limitation of the "low-foot" class of implosions appears to have been mix. Lower convergence "high-foot" implosions are found to be less susceptible to mix, allowing velocities of >380 km/s to be achieved.

  17. The critical parameters of the thermal explosion micro hot-spot model dependence on the pulse duration

    Science.gov (United States)

    Kalenskii, A. V.; Zvekov, A. A.; Galkina, E. V.

    2017-05-01

    The dependencies of critical laser initiation energy density of pentaerythritol tetranitrate (PETN) - aluminum nanoparticles, PETN - cobalt nanoparticles and lead azide - lead nanoparticles on pulse duration were calculated in terms of the refined micro hot-spot model. It was shown that the absorption efficiency of the laser irradiation taken into account makes the initiation criterion change. According to the calculation results, the criterion in the limit of short pulses is energy density matching the experimental data. If the neodymium pulses duration is less than 50 ns, the radius of the nanoparticles with highest temperature varies insignificantly. The expression for the critical hot-spot temperature dependence on the pulse duration was derived. The conclusion was made that the model refining with nanoparticles absorption efficiency dependence on their radius is sufficient for the “small particles’ paradox” solution.

  18. Detection and analysis of the hot spots of ATP7B gene mutation in the members of a family with an adult Wilson's disease%成人Wilson病一家系成员P型铜转运三磷酸腺苷酶基因突变热区的检测和分析

    Institute of Scientific and Technical Information of China (English)

    朱永豹; 黄元成; 郭威; 陈韬

    2012-01-01

    Objective To make a early gene diagnosis and analyze the mutation characteristics of ATP7B gene by detecting the hot spots of ATP7B gene mutation in Chinese family patients with Wilson's disease. Methods The genomic DNA of the family members were extracted from peripheral blood. The fragments of exon 8,1243 of ATP7B gene were amplified by polymerase chain reaction and PCR products were sequenced bidirectionally. Then we analyzed the results with BLAST software online. Results I 1N I 2( proband) , II1 and II2 have heterozygote missense mutation Arg778Leu(2333G > T) and heterozy-gote nonsense mutation Leu770Leu (2310C>G) on exon 8. I 1 、 I 2, I 3 and II1 have heterozygote mis-sense mutation Lys952Arg(2855A >G) on exon 12. All the subjects have no mutations on exon 13. Conclusion Exon 8、12,13 of ATP7B gene should be detected in the members of a family with a proband having Wilson' s disease, which will help to diagnose pre-symptomatic patients and carriers earlier.%目的 通过检测中国人Wilson病(WD)P型铜转运三磷酸腺苷酶(ATP7B)基因突变热区,对成人WD一家系成员进行早期基因诊断和突变特征分析.方法 提取该家系成员外周血基因组DNA,采用聚合酶链反应扩增ATP7B基因第8、12、13号外显子,并对扩增产物进行直接双向测序,然后应用在线BLAST软件分析.结果 Ⅰ1、Ⅰ2(先证者)、Ⅱ1和Ⅱ2第8号外显子存在Arg778Leu(2333G> T)错义杂合突变,且均伴有Leu770Leu( 2310C>G)同义杂合突变;Ⅰ1、Ⅰ 2、Ⅰ3和Ⅱ1第12号外显子存在Lys952Arg(2855A>G)错义杂合突变;所有受检者第13号外显子均未存在突变.结论 对有先证者的Wilson病家系成员应进行ATP7B基因第8、12、13号外显子检测,有助于早期发现症状前患者和携带者.

  19. A comparison of Ki-67 counting methods in luminal Breast Cancer: The Average Method vs. the Hot Spot Method

    Science.gov (United States)

    Jang, Min Hye; Kim, Hyun Jung; Chung, Yul Ri; Lee, Yangkyu

    2017-01-01

    In spite of the usefulness of the Ki-67 labeling index (LI) as a prognostic and predictive marker in breast cancer, its clinical application remains limited due to variability in its measurement and the absence of a standard method of interpretation. This study was designed to compare the two methods of assessing Ki-67 LI: the average method vs. the hot spot method and thus to determine which method is more appropriate in predicting prognosis of luminal/HER2-negative breast cancers. Ki-67 LIs were calculated by direct counting of three representative areas of 493 luminal/HER2-negative breast cancers using the two methods. We calculated the differences in the Ki-67 LIs (ΔKi-67) between the two methods and the ratio of the Ki-67 LIs (H/A ratio) of the two methods. In addition, we compared the performance of the Ki-67 LIs obtained by the two methods as prognostic markers. ΔKi-67 ranged from 0.01% to 33.3% and the H/A ratio ranged from 1.0 to 2.6. Based on the receiver operating characteristic curve method, the predictive powers of the KI-67 LI measured by the two methods were similar (Area under curve: hot spot method, 0.711; average method, 0.700). In multivariate analysis, high Ki-67 LI based on either method was an independent poor prognostic factor, along with high T stage and node metastasis. However, in repeated counts, the hot spot method did not consistently classify tumors into high vs. low Ki-67 LI groups. In conclusion, both the average and hot spot method of evaluating Ki-67 LI have good predictive performances for tumor recurrence in luminal/HER2-negative breast cancers. However, we recommend using the average method for the present because of its greater reproducibility. PMID:28187177

  20. Imaging challenges in 20nm and 14nm logic nodes: hot spots performance in Metal1 layer

    Science.gov (United States)

    Timoshkov, V.; Rio, D.; Liu, H.; Gillijns, W.; Wang, J.; Wong, P.; Van Den Heuvel, D.; Wiaux, V.; Nikolsky, P.; Finders, J.

    2013-10-01

    The 20nm Metal1 layer, based on ARM standard cells, has a 2D design with minimum pitch of 64nm. This 2D design requires a Litho-Etch-Litho-Etch (LELE) double patterning. The whole design is divided in 2 splits: Me1A and Me1B. But solution of splitting conflicts needs stitching at some locations, what requires good Critical Dimension (CD) and overlay control to provide reliable contact between 2 stitched line ends. ASML Immersion NXT tools are aimed at 20 and 14nm logic production nodes. Focus control requirements become tighter, as existing 20nm production logic layouts, based on ARM, have about 50-60nm focus latitude and tight CD Uniformity (CDU) specifications, especially for line ends. IMEC inspected 20nm production Metal1 ARM standard cells with a Negative Tone Development (NTD) process using the Process Window Qualification-like technique experimentally and by Brion Tachyon LMC by simulations. Stronger defects were found thru process variations. A calibrated Tachyon model proved a good overall predictability capability for this process. Selected defects are likely to be transferred to hard mask during etch. Further, CDU inspection was performed for these critical features. Hot spots showed worse CD uniformity than specifications. Intra-field CDU contribution is significant in overall CDU budget, where reticle has major impact due to high MEEF of hot spots. Tip-to-Tip and tip-to-line hot spots have high MEEF and its variation over the field. Best focus variation range was determined by best focus offsets between hot spots and its variation within the field.

  1. X-ray Radiation Mechanisms and the Beaming Effect of Hot Spots and Knots in AGN Jets

    Indian Academy of Sciences (India)

    Jin Zhang; Jin-Ming Bai; Liang Chen; Enwei Liang

    2011-03-01

    The observed broadband spectral energy distributions (SEDs) of 22 hot spots and 45 knots are modelled with single-zone lepton models. Considering the sources at rest, the X-rays of some hot spots can be explained by the SSC model with magnetic field being consistent with the equipartition magnetic field in magnitude of order 1, but at the same time an unreasonably low magnetic field is required to model the X-rays for all knots. When considering the relativistic bulk motion of the sources, the IC/CMB model well explains the X-ray emission for most of them under the equipartition condition. We show that the ratio of observational luminosity is tentatively correlated with the co-moving equipartition magnetic field 'eq and the beaming factor . These facts suggest that the observational differences of the X-rays from the knots and hot spots may be mainly due to the differences in the Doppler boosting effect and the co-moving magnetic field of the two kinds of source.

  2. Comparative transcriptome resources of eleven Primulina species, a group of 'stone plants' from a biodiversity hot spot.

    Science.gov (United States)

    Ai, Bin; Gao, Yong; Zhang, Xiaolong; Tao, Junjie; Kang, Ming; Huang, Hongwen

    2015-05-01

    The genus Primulina is an emerging model system in studying the drivers and mechanisms of species diversification, for its high species richness and endemism, together with high degree of habitat specialization. In this study, we sequenced transcriptomes for eleven Primulina species across the phylogeny of the genus using the Illumina HiSeq 2000 platform. A total of 336 million clean reads were processed into 355 573 unigenes with a mean length of 1336 bp and an N50 value of 2191 bp after pooling and reassembling twelve individual pre-assembled unigene sets. Of these unigenes, 249 973 (70%) were successfully annotated and 256 601 (72%) were identified as coding sequences (CDSs). We identified a total of 38 279 simple sequence repeats (SSRs) and 367 123 single nucleotide polymorphisms (SNPs). Marker validation assay revealed that 354 (27.3%) of the 1296 SSR and 795 (39.6%) of the 2008 SNP loci showed successful genotyping performance and exhibited expected polymorphism profiles. We screened 834 putative single-copy nuclear genes and proved their high effectiveness in phylogeny construction and estimation of ancestral population parameters. We identified a total of 85 candidate orthologs under positive selection for 46 of the 66 species pairs. This study provided an efficient application of RNA-seq in development of genomic resources for a group of 'stone plants' from south China Karst regions, a biodiversity hot spot of the World. The assembled unigenes with annotations and the massive gene-associated molecular markers would help guide further molecular systematic, population genetic and ecological genomics studies in Primulina and its relatives. © 2014 John Wiley & Sons Ltd.

  3. WiFi Hot Spot Service Business for the Automotive and Oil Industries: A Competitive Analysis

    Directory of Open Access Journals (Sweden)

    Louis-Francois PAU

    2010-01-01

    Full Text Available While you refuel for gas, why not refuel for information or upload vehicle data, using a cheap wireless technology as WiFi? This paper analyzes in extensive detail the user segmentation by vehicle usage, service offering, and full business models from WiFi hot spot services delivered to and from vehicles (private, professional, public around gas stations. Are also analyzed the parties which play a role in such services: authorization, provisioning and delivery, with all the dependencies modelled by attributed digraphs. Account is made of WiFi base station technical capabilities and costs. Five year financial models (CAPEX, OPEX, and data pertain to two possible service suppliers: multi-service oil companies, and mobile service operators (or MVNOs. Model optimization on the return-on-investment (R.O.I. is carried out for different deployment scenarios, geographical coverage assumptions, as well as tariff structures. Comparison is also being made with public GPRS and 3G data services, as precursors to HSPA/LTE, and the effect of WiFi roaming is analyzed. Regulatory implications, including those dealing with public safety, are addressed. Analysis shows that due to manpower costs and marketing costs, suitable R.O.I. will not be achieved unless externalities are accounted for and innovative tariff structures are introduced. Open issues and further research are outlined. Further work is currently carried out with automotive electronics sector, wireless systems providers, wireless terminals platform suppliers, and vehicle manufacturers. Future relevance of this work is also discussed for the emerging electrical reloading grids for electrical vehicles.

  4. Electric arc furnaces for steel-making: hot spots for persistent organic pollutants.

    Science.gov (United States)

    Odabasi, Mustafa; Bayram, Abdurrahman; Elbir, Tolga; Seyfioglu, Remzi; Dumanoglu, Yetkin; Bozlaker, Ayse; Demircioglu, Hulusi; Altiok, Hasan; Yatkin, Sinan; Cetin, Banu

    2009-07-15

    Persistent organic pollutant (POP) concentrations were measured in stack-gases of ferrous scrap processing steel plants with electric arc furnaces (EAFs) (n = 5) in Aliaga, Izmir, Turkey and in air (n = 11) at a site near those plants. Measured stack-gas concentrations for the four plants without scrap preheating (611 +/- 311, 165,000 +/- 285,000, and 33 +/- 3 ng m(-3), average +/- SD for sigma41PCBs, sigma16PAHs, and sigma7PBDEs, respectively) indicated that they are significant sources for polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs). POP emissions from the plant with scrap preheating were significantly higher (13 500, 445 000, and 91 ng m(-3) for sigma41PCBs, sigma16PAHs, and sigma7PBDEs, respectively). It was also shown that the steel plants emit considerable amounts of fugitive POPs in particle-phase. Estimated emissions using the emission factors generated in this study and the production amounts suggested that the steel plants with EAFs may significantly contribute to local and global PAH, PCB, and PBDE emissions. Several other compounds (aromatic and aliphatic hydrocarbons, oxygen, sulfur, nitrogen, and chlorine-containing organic compounds, n = 49) were identified and determined semiquantitatively in the stack-gas and ambient air samples. Ambient air concentrations (62 +/- 35, 320 +/- 134 ng m(-3), 1451 +/- 954 pg m(-3), for sigma41PCBs, sigma16PAHs, and sigma7PBDEs, respectively) were significantly higher than those measured previously around the world and in the region, further confirming that the steel plants with EAFs are "hot spots" for POPs.

  5. Relating Precipitation Phenomena with MODIS Detected Hot Spots in the Maritime Continent

    Science.gov (United States)

    Ramirez, E. M.; Reid, J. S.; Xian, P.; Hyer, E.; Turk, J.; Flatau, M.; Zhang, C.

    2009-12-01

    Recent studies of land use practices in SE Asia’s Maritime Continent (MC) have raised questions over potential meteorological implications including smoke-cloud interaction and changes in the regional radiation budget. Land management practices on Java, Sumatra, Sulawesi, Borneo, and the Malay Peninsula include biomass burning to clear primary forest as well as to maintain oil palm plantations. Burning is also employed for clearing unwanted remains from previous crops, rice stubble for example. However, burning activity is often dictated by weather, in particular precipitation. We studied 5 years of MODIS active fire hot spot and satellite precipitation data to investigate how observed burning activity correlated with precipitation features at four major scales: 1) Intraseasonal El Nino-Southern Oscillation (ENSO) and Indian Ocean Dipole (IOP); 2) Seasonal migration of the Intertropical Convergence Zone (ITCZ); 3) the 30-90 day Madden Julian Oscillation (MJO); and 4) regional convection from localized weather phenomenon (e.g., orographic, isolated thermal convection, sea breeze, etc…). It was found that observed burn patterns from each of the islands of the MC had differing responses to these four forcings. Observed burning activity on the islands of Borneo and Sulawesi correlated strongly with the ENSO and ITCZ indicies, whereas Sumatra was more influenced by the phase and strength of the MJO. Java, showing a mix of influence, exhibited strong fire activity in the morning rather than afternoon which is unusual for most burning regions. We hypothesize that these observed relationships reflect both physical land use differences and contextual bias/observability issues associated with the region’s heavy cloud cover. For further studies on smoke-meteorology interaction, our findings point to the need for a clear understanding of the meteorological context of satellite observations.

  6. Hot Spots of Mercury Bioaccumulation in Amphibian Populations From the Conterminous United States

    Science.gov (United States)

    Bank, M. S.

    2008-12-01

    Mercury (Hg) contamination in the United States (U.S.) is well-documented and continues to be a public- health issue of great concern. Fish consumption advisories have been issued throughout much of the U.S. due to elevated levels of methylmercury (MeHg). Methylmercury contamination in the developing fetus and in young children is a major public health issue for certain sectors of the global human population. Moreover, identifying MeHg hot spots and the effects of MeHg pollution on environmental health and biodiversity are also considered a high priority for land managers, risk assessors, and conservation scientists. Despite their overall biomass and importance to aquatic and terrestrial ecosystems, Hg and MeHg bioaccumulation dynamics and toxicity in amphibians are not well studied, especially when compared to other vertebrate taxa such as birds, mammals, and fish species. Population declines in amphibians are well documented and likely caused by synergistic and interacting, multiple stressors such as climate change, exposure to toxic pollutants, fungal pathogens, and habitat loss and ecosystem degradation. Protecting quality of terrestrial ecosystems in the U.S. has enormous ramifications for economic and public health of the nation's residents and is fundamental to maintaining the biotic integrity of surface waters, riparian zones, and environmental health of forested landscapes nationwide. Determining Hg concentration levels for terrestrial and surface water ecosystems also has important implications for protecting the nation's fauna. Here I present an overview of the National Amphibian Mercury Program and evaluate variation in MeHg hotspots, Hg bioaccumulation and distribution in freshwater and terrestrial habitats across a broad gradient of physical, climatic, biotic, and ecosystem settings to identify the environmental conditions and ecosystem types that are most sensitive to Hg pollution. The role of geography, disturbance mechanisms, and abiotic and biotic

  7. Seamount morphology in the Bowie and Cobb hot spot trails, Gulf of Alaska

    Science.gov (United States)

    Chaytor, Jason D.; Keller, Randall A.; Duncan, Robert A.; Dziak, Robert P.

    2007-09-01

    Full-coverage multibeam bathymetric mapping of twelve seamounts in the Gulf of Alaska reveals that they are characterized by flat-topped summits (rarely with summit craters) and by terraced, or step-bench, flanks. These summit plateaus contain relict volcanic features (e.g., flow levees, late-stage cones, and collapse craters) and as such must have been constructed by volcanic processes such as lava ponding above a central vent, rather than by erosion above sea level. The terraced flanks are composed of a sequence of stacked lava deltas and cones, probably tube-fed from a central lava pond, a morphology which is suggestive of long-lived, stable central lava sources and low to moderate eruption rates, indicative of significant time spent above a hot spot outlet. Most of these seamounts have summit plateaus surrounded, and cut into, by amphitheater headwall scarps, and flanks that are scarred by debris chutes, but lack visible debris accumulations at their base. We interpret the lack of blocky debris fields as evidence that the slope failures are mainly small-scale debris flows, rather than large-scale flank collapses. However, we cannot rule out the possibility that large flank-collapse blocks from early in the histories of these seamounts are now hidden beneath the thick glacio-fluvial fan deposits that cover the Gulf of Alaska seafloor. These slope failure features become smoother and longer and increase in size and abundance with increasing age of a seamount, suggesting that slope failure processes continue long after volcanic activity ceases.

  8. Cooling water of power plant creates "hot spots" for tropical fishes and parasites.

    Science.gov (United States)

    Emde, Sebastian; Kochmann, Judith; Kuhn, Thomas; Dörge, Dorian D; Plath, Martin; Miesen, Friedrich W; Klimpel, Sven

    2016-01-01

    Thermally altered water bodies can function as "hot spots" where non-native species are establishing self-sustaining populations beyond their tropical and subtropical native regions. Whereas many tropical fish species have been found in these habitats, the introduction of non-native parasites often remains undetected. Here, n = 77 convict cichlids (Amatitlania nigrofasciata) were sampled by electro-fishing at two sites from a thermally altered stream in Germany and examined for parasite fauna and feeding ecology. Stomach content analysis suggests an opportunistic feeding strategy of A. nigrofasciata: while plant material dominated the diet at the warm water inlet (∼30 °C), relative contributions of insects, plants, and crustaceans were balanced 3 km downstream (∼27 °C). The most abundant non-native parasite species was the tropical nematode Camallanus cotti with P = 11.90 % and P = 80.00 % at the inlet and further downstream, respectively. Additionally, nematode larvae of Anguillicoloides crassus and one specimen of the subtropical species Bothriocephalus acheilognathi were isolated. A. nigrofasciata was also highly infected with the native parasite Acanthocephalus anguillae, which could be linked to high numbers of the parasite's intermediate host Asellus aquaticus. The aim of this study was to highlight the risk and consequences of the release and establishment of ornamental fish species for the introduction and spread of non-indigenous metazoan parasites using the convict cichlid as a model species. Furthermore, the spread of non-native parasites into adjacent fish communities needs to be addressed in the future as first evidence of Camallanus cotti in native fish species was also found.

  9. Isocitrate dehydrogenase mutation hot spots in acute lymphoblastic leukemia and oral cancer

    Directory of Open Access Journals (Sweden)

    Jen-Yang Tang

    2012-03-01

    Full Text Available Isocitrate dehydrogenase (IDH encodes a nicotinamide adenine dinucleotide phosphate+-dependent enzyme for oxidative decarboxylation of isocitrate and has an essential role in the tricarboxylic acid cycle. Mutations of IDH1 and IDH2 have been identified in patients with glioma, leukemia, and other cancers. However, the incidence of IDH mutations in acute myeloid leukemia in Taiwan is much lower than that reported in Western countries. The reason for the difference is unknown and its clinical implications remain unclear. Acute lymphoblastic leukemia (ALL is a heterogenous hematopoietic malignancy. Oral squamous cell carcinoma (OSCC results from chronic carcinogen exposures and is highly prevalent in trucking workers, especially in southern Taiwan. Subtypes of both diseases require specific treatments, and molecular markers for developing tailored treatments are limited. High-resolution melting (HRM analysis is now a widely used methodology for rapid, accurate, and low-cost mutation scanning. In this study, 90 adults with OSC and 31 children with ALL were scanned by HRM analysis for IDH1 and IDH2 mutation hot spots. In ALL, the allele frequency was 3.23% in both IDH1 and IDH2. In OSCC, the allele frequency was 2.22% in IDH2. A synonymous mutation over pG313 (c.939A > G of IDH2 was found in both pediatric ALL and adult OSCC. Therefore, we concluded that mutations of IDH are uncommon in ALL and OSCC and are apparently not a major consideration when selecting treatment modalities.

  10. A study of the long-term properties of Jovian hot spots from HST and ground-based observations between 1994 and 1998

    Science.gov (United States)

    Arregui, E.; Rojas, J. F.; Sanchez-Lavega, A.; Lecacheux, J.; Colas, F.; Miyazaki, I.; Parker, D.

    2000-10-01

    We have used the HST-WFPC2 archived images of Jupiter in the period 1994-1998 together with a large set of CCD ground based images, to study the zonal distribution, long-term motions, lifetimes, interactions and other properties of the hot spot - plume regions at 7 degrees North. Red and near infrared filters covering the wavelength range 650 - 953 nm have been used since they show the hot spots with a high contrast. We have found that the hot spots have velocities ranging from 95 to 112 m/s and are grouped typically in families of three to six members. We do not found any correlation between their velocity and wavenumber. The long-term survey allowed us to identify mergers and splitting of the hot spots areas. The Spanish team was supported by Gobierno Vasco PI 034/97. The French team was supported by the "Programme National de Planetologie."

  11. Effect of width, amplitude, and position of a core mantle boundary hot spot on core convection and dynamo action

    CERN Document Server

    Dietrich, Wieland; Hori, Kumiko

    2015-01-01

    Within the fluid iron cores of terrestrial planets, convection and the resulting generation of global magnetic fields are controlled by the overlying rocky mantle. The thermal structure of the lower mantle determines how much heat is allowed to escape the core. Hot lower mantle features, such as the thermal footprint of a giant impact or hot mantle plumes, will locally reduce the heat flux through the core mantle boundary (CMB), thereby weakening core convection and affecting the magnetic field generation process. In this study, we numerically investigate how parametrised hot spots at the CMB with arbitrary sizes, amplitudes, and positions affect core convection and hence the dynamo. The effect of the heat flux anomaly is quantified by changes in global flow symmetry properties, such as the emergence of equatorial antisymmetric, axisymmetric (EAA) zonal flows. For purely hydrodynamic models, the EAA symmetry scales almost linearly with the CMB amplitude and size, whereas self-consistent dynamo simulations typ...

  12. The west side story: MEFV haplotype in Spanish FMF patients and controls, and evidence of high LD and a recombination "hot-spot" at the MEFV locus.

    Science.gov (United States)

    Aldea, Anna; Calafell, Francesc; Aróstegui, Juan I; Lao, Oscar; Rius, Josefa; Plaza, Susana; Masó, Montserrat; Vives, Jordi; Buades, Joan; Yagüe, Jordi

    2004-04-01

    Mutations at the MEFV gene cause, with various degrees of penetrance, familial Mediterranean fever (FMF). This disease is more prevalent in the Middle East than elsewhere, and most studies have focused on those populations. However, FMF occurs also in the Western Mediterranean and these populations should be taken into account for a complete view of FMF. We have analyzed intragenic MEFV SNPs in Spanish and Chueta (descendants of converted Jews) FMF patients and controls, and this constitutes the first systematic survey of normal MEFV SNP haplotype structure and variability. Our findings have allowed us to systematize the nomenclature of MEFV haplotypes and show that there is strong linkage disequilibrium (LD) at the MEFV locus and an intragenic recombination hot spot. The high local LD, regardless the recombination hot spot, is responsible for the limited diversity of the MEFV control haplotypes found in the Spanish population and it suggests that it may be a common feature to all Mediterranean populations. The MEFV mutation spectrum in Spain is quite diverse, and similar to those of France and Italy. On the contrary, the Chueta spectrum was poorer and closer to that of North African Jews, suggesting a direct connection with the Jewish diaspora.

  13. Origin of the X-rays and Possible GeV-TeV Emission from the Western Hot Spot Of Pictor A

    CERN Document Server

    Zhang, Jin; Chen, Liang; Yang, Xian

    2009-01-01

    Pictor A is a nearby Fanaroff-Riley class II (FR II) radio galaxy with a bright hot spot, the western hot spot. Observation of high polarization in the optical emission of the hot spot indicates that the optical emission could be synchrotron radiation of relativistic electrons in the hot spot. These electrons may be able to produce high energy gamma-ray photons through inverse Compton (IC) scattering. We use single-zone and multi-zone synchrotron + synchrotron-self-Compton (SSC) models to fit the observed spectral energy distribution (SED) from the radio to the X-ray band of the hot spot. Our esults show that in the case of a much weaker magnetic field strength than the equipartition magnetic field, both the single-zone and multi-zone models can fit the SED, but the multi-zone model significantly improves the fit. The two models predict the hot spot as a GeV-TeV source, which might be marginally detectable with Fermi/LAT and HESS. The inverse Compton scattering of cosmic microwave background (IC/CMB) is also ...

  14. A Radio through X-ray Study of the Hot Spots, Active Nucleus, and Environment of the Nearby FR II Radio Galaxy 3C 33

    CERN Document Server

    Kraft, R P; Hardcastle, M J; Evans, D A; Croston, J H; Worrall, D M; Murray, S S

    2007-01-01

    We present results from {\\em Chandra}/ACIS-S, {\\em Spitzer}, {\\em XMM-Newton}, {\\em HST}, and VLA observations of the radio hot spots, extended environment, and nucleus of the nearby ($z$=0.0597) FR II radio galaxy 3C 33. This is a relatively low-power FR II radio galaxy, and so we expect, {\\it a priori}, to detect a significant X-ray synchrotron component to the emission from the hot spots. We detect X-ray emission coincident with the two knots of polarized optical emission from the southern hot spot (SHS), as well as along the northwest arm of this hot spot. We also detect X-ray emission from two compact regions of the northern hot spot (NHS), as well as diffuse emission behind the radio peak. The X-ray flux density of the region at the tip of the southern hot spot, the most compact radio feature of the southern lobe, is consistent with the synchrotron self-Compton (SSC) process. The X-ray flux densities of the other three regions of the SHS and the two compact regions of the NHS are an order of magnitude o...

  15. Mapping Cultural Ecosystem Services in Vilnius using Hot-Spot Analysis.

    Science.gov (United States)

    Pereira, Paulo; Depellegrin, Daniel; Egarter-Vigl, Lukas; Oliva, Marc; Misiune, Ieva; Keesstra, Saskia; Estebaranz, Ferran; Cerda, Artemi

    2017-04-01

    Cultural services in urban areas are very important to promote tourism activities and develop the economy. These activities are fundamental for the sustainability of the urban areas since can represent an important monetary source. However, one of the major threats to the sustainability of cultural services is the high amount of visitants that can lead to a degradation of the services provided (Depellegrin et al., 2016). Mapping the potential of cultural ecosystems services is fundamental to assess the capacity that the territory have to provide it. Previous works used land use classification to identify the ecosystem services potential, and revealed to be a good methodology to attribute to each type of land use a specific capacity (Burkhard et al., 2008). The objective of this work is to map the cultural services in Vilnius area using a hot-spot analysis. Ecosystem services potential was assessed using the matrix developed by Burkhard et al. (2009), which ranks ES capacity from 0= no capacity to 5=very high relevant capacity to a different land use type. The results showed that with the exception of Cultural Heritage ecosystem services that had a random pattern (Z-score=0.62, pTourism (Z-score=4.02, pTourism ecosystem services had the maximum spatial correlation at the distance of 5125.12 m, Landscape Aesthetics at 3495.70 m, Knowledge Systems at 5218.66 m, Religious and Spiritual at 3495.70 m, Cultural Heritage at 6746.17 m and Natural Heritage at 6205.82 m. This showed that the cultural services studied have a different spatial correlation. References Burkhard B, Kroll F, Müller F, Windhorst W. 2009. Landscapes' capacities to provide ecosystem services- a concept for land-cover based assessments. Landscape Online. 15, 1-22. Depellegrin, D.A., Pereira, P., Misiune, I., Egarter-Vigl, L. Mapping Ecosystem Services potential in Lithuania. International Journal of Sustainable Development and World Ecology, 23, 441-455.

  16. Hot Spots and Mantle Plumes: A Window Into the Deep Earth and a Lesson on How Science Really Works

    Science.gov (United States)

    Caplan-Auerbach, J.

    2010-12-01

    Despite years of discussion, debate and controversy over the causes of ocean island volcanism, most students simply learn that such features form from fixed plumes of hot material rising from the core mantle boundary. Although we know that the Hawaiian plume exhibited substantial southward motion, most introductory geology textbooks still report that hot spots are fixed and that the Hawaiian-Emperor bend reflects a change in plate motion. That mantle plumes are the focus of significant controversy within the scientific community is rarely, if ever, discussed, and alternative models for the formation of intraplate volcanoes are ignored. Students may thus complete their studies without learning about the dynamic debate focused on the existence and formation of mantle plumes. This issue represents an opportunity for students to see how science really works, how new models are constructed, and what distinguishes a hypothesis from a theory. The culminating project in Western Washington University’s Introduction to Geophysics class, a course required for the BS degree in geology, focuses on the hot spot and mantle plume debate. For the first nine weeks of the quarter students learn about general topics in geophysics including plate tectonics, magnetism, seismology, gravity and heat flow. At the end of the course, students break into small research groups with the goal of investigating how geophysics may be used to address three questions: (1) Do ocean island volcanoes form from mantle plumes? (2) Are “hot spots” actually hot? (3) Are hot spots stationary? Each group examines how these questions may be addressed using a specific geophysical tool. In addition to the five topics described above, a sixth group investigates the question of “if not hot spots/mantle plumes, how do ocean island volcanoes form?” Students read the current literature on the topic and present their results to their classmates. Presentations focus on topics such as the use of seismic

  17. Inverse gene-for-gene interactions contribute additively to tan spot susceptibility in wheat

    Science.gov (United States)

    Tan spot of wheat, caused by Pyrenophora tritici-repentis, is an important disease in almost all wheat-growing areas of the world. The disease system is known to involve at least three fungal-produced necrotrophic effectors (NEs) that interact with corresponding host sensitivity (S) genes in an inv...

  18. Construction of a multiplex mutation hot spot PCR panel: the first step towards colorectal cancer genotyping on the GS Junior platform

    Science.gov (United States)

    Péterfia, Bálint; Kalmár, Alexandra; Patai, Árpád V.; Csabai, István; Bodor, András; Micsik, Tamás; Wichmann, Barnabás; Egedi, Krisztina; Hollósi, Péter; Kovalszky, Ilona; Tulassay, Zsolt; Molnár, Béla

    2017-01-01

    Background: To support cancer therapy, development of low cost library preparation techniques for targeted next generation sequencing (NGS) is needed. In this study we designed and tested a PCR-based library preparation panel with limited target area for sequencing the top 12 somatic mutation hot spots in colorectal cancer on the GS Junior instrument. Materials and Methods: A multiplex PCR panel was designed to amplify regions of mutation hot spots in 12 selected genes (APC, BRAF, CTNNB1, EGFR, FBXW7, KRAS, NRAS, MSH6, PIK3CA, SMAD2, SMAD4, TP53). Amplicons were sequenced on a GS Junior instrument using ligated and barcoded adaptors. Eight samples were sequenced in a single run. Colonic DNA samples (8 normal mucosa; 33 adenomas; 17 adenocarcinomas) as well as HT-29 and Caco-2 cell lines with known mutation profiles were analyzed. Variants found by the panel on APC, BRAF, KRAS and NRAS genes were validated by conventional sequencing. Results: In total, 34 kinds of mutations were detected including two novel mutations (FBXW7 c.1740:C>G and SMAD4 c.413C>G) that have not been recorded in mutation databases, and one potential germline mutation (APC). The most frequently mutated genes were APC, TP53 and KRAS with 30%, 15% and 21% frequencies in adenomas and 29%, 53% and 29% frequencies in carcinomas, respectively. In cell lines, all the expected mutations were detected except for one located in a homopolymer region. According to re-sequencing results sensitivity and specificity was 100% and 92% respectively. Conclusions: Our NGS-based screening panel denotes a promising step towards low cost colorectal cancer genotyping on the GS Junior instrument. Despite the relatively low coverage, we discovered two novel mutations and obtained mutation frequencies comparable to literature data. Additionally, as an advantage, this panel requires less template DNA than sequence capture colon cancer panels currently available for the GS Junior instrument.

  19. The southern stratospheric gravity-wave hot spot: individual waves and their momentum fluxes measured by COSMIC GPS-RO

    Directory of Open Access Journals (Sweden)

    N. P. Hindley

    2015-02-01

    Full Text Available During austral winter the mountains of the southern Andes and Antarctic Peninsula are a known hot spot of intense gravity wave momentum flux. There also exists a long leeward region of increased gravity wave energy that sweeps eastwards from the mountains out over the Southern Ocean, the source of which has historically proved difficult to determine. In this study we use Global Positioning System (GPS Radio Occultation (RO data from the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC satellite constellation to investigate the distribution, variability and sources of waves in the hot spot region and over the Southern Ocean. We present evidence that suggests a southward focusing of waves into the stratospheric jet from sources to the north. We also describe a wavelet analysis technique for the quantitative identification of individual waves from COSMIC temperature profiles. This analysis reveals different geographical regimes of wave amplitude and short-timescale variability in the wave field over the Southern Ocean. Finally, we take advantage of the large numbers of closely spaced pairs of profiles from the deployment phase of the COSMIC constellation in 2006 to make estimates of gravity wave horizontal wavelengths. We show that, given sufficient numbers of these pairs, GPS-RO can then produce physically reasonable estimates of stratospheric gravity wave momentum flux in the hot spot region that are consistent with other studies. The results are discussed in the context of previous satellite and modelling studies to build up a better picture of the nature and origins of waves in the southern winter stratosphere.

  20. Temporary shielding of hot spots in the drainage areas of cutaneous melanoma improves accuracy of lymphoscintigraphic sentinel lymph node diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Maza, S.; Valencia, R.; Geworski, L.; Zander, A.; Munz, D.L. [Clinic for Nuclear Medicine, University Hospital Charite, Humboldt University of Berlin, Schumannstrasse 20-21, 10117 Berlin (Germany); Draeger, E.; Winter, H.; Sterry, W. [Clinic for Dermatology, Venereology and Allergology, University Hospital Charite, Humboldt University of Berlin, Berlin (Germany)

    2002-10-01

    Detection of the ''true'' sentinel lymph nodes, permitting correct staging of regional lymph nodes, is essential for management and prognostic assessment in malignant melanoma. In this study, it was prospectively evaluated whether simple temporary shielding of hot spots in lymphatic drainage areas could improve the accuracy of sentinel lymph node diagnostics. In 100 consecutive malignant melanoma patients (45 women, 55 men; age 11-91 years), dynamic and static lymphoscintigraphy in various views was performed after strict intracutaneous application of technetium-99m nanocolloid (40-150 MBq; 0.05 ml/deposit) around the tumour (31 patients) or the biopsy scar (69 patients, safety distance 1 cm). The images were acquired with and without temporary lead shielding of the most prominent hot spots in the drainage area. In 33/100 patients, one or two additional sentinel lymph nodes that showed less tracer accumulation or were smaller (<1.5 cm) were detected after shielding. Four of these patients had metastases in the sentinel lymph nodes; the non-sentinel lymph nodes were tumour negative. In 3/100 patients, hot spots in the drainage area proved to be lymph vessels, lymph vessel intersections or lymph vessel ectasias after temporary shielding; hence, a node interpreted as a non-sentinel lymph node at first glance proved to be the real sentinel lymph node. In two of these patients, lymph node metastasis was histologically confirmed; the non-sentinel lymph nodes were tumour free. In 7/100 patients the exact course of lymph vessels could be mapped after shielding. In one of these patients, two additional sentinel lymph nodes (with metastasis) were detected. Overall, in 43/100 patients the temporary shielding yielded additional information, with sentinel lymph node metastases in 7%. In conclusion, when used in combination with dynamic acquisition in various views, temporary shielding of prominent hot spots in the drainage area of a malignant melanoma of the

  1. Bear Creek Valley Floodplain Hot Spot Removal Action Project Plan, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    The Bear Creek Valley Floodplain Hot Spot Removal Action Project Plan, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee (Y/ER-301) was prepared (1) to safely, cost-effectively, and efficiently evaluate the environmental impact of solid material in the two debris areas in the context of industrial land uses (as defined in the Bear Creek Valley Feasibility Study) to support the Engineering Evaluation/Cost Assessment and (2) to evaluate, define, and implement the actions to mitigate these impacts. This work was performed under Work Breakdown Structure 1.x.01.20.01.08.

  2. Finite volume analysis of temperature effects induced by active MRI implants: 2. Defects on active MRI implants causing hot spots

    Directory of Open Access Journals (Sweden)

    Grönemeyer Dietrich HW

    2006-05-01

    Full Text Available Abstract Background Active magnetic resonance imaging implants, for example stents, stent grafts or vena cava filters, are constructed as wireless inductively coupled transmit and receive coils. They are built as a resonator tuned to the Larmor frequency of a magnetic resonance system. The resonator can be added to or incorporated within the implant. This technology can counteract the shielding caused by eddy currents inside the metallic implant structure. This may allow getting diagnostic information of the implant lumen (in stent stenosis or thrombosis for example. The electro magnetic rf-pulses during magnetic resonance imaging induce a current in the circuit path of the resonator. A by material fatigue provoked partial rupture of the circuit path or a broken wire with touching surfaces can set up a relatively high resistance on a very short distance, which may behave as a point-like power source, a hot spot, inside the body part the resonator is implanted to. This local power loss inside a small volume can reach ¼ of the total power loss of the intact resonating circuit, which itself is proportional to the product of the resonator volume and the quality factor and depends as well from the orientation of the resonator with respect to the main magnetic field and the imaging sequence the resonator is exposed to. Methods First an analytical solution of a hot spot for thermal equilibrium is described. This analytical solution with a definite hot spot power loss represents the worst case scenario for thermal equilibrium inside a homogeneous medium without cooling effects. Starting with this worst case assumptions additional conditions are considered in a numerical simulation, which are more realistic and may make the results less critical. The analytical solution as well as the numerical simulations use the experimental experience of the maximum hot spot power loss of implanted resonators with a definite volume during magnetic resonance imaging

  3. Bering-Okhotsk Seal Survey (BOSS) Identified Hot Spots (2012-13)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — US surveys were conducted of the Bering Sea pack ice for bearded, spotted, ribbon, and ringed seals using digital cameras and thermal imagers mounted in the belly...

  4. Hot spot formation and chemical reaction initiation in shocked HMX crystals with nanovoids: a large-scale reactive molecular dynamics study.

    Science.gov (United States)

    Zhou, Tingting; Lou, Jianfeng; Zhang, Yangeng; Song, Huajie; Huang, Fenglei

    2016-07-14

    We report million-atom reactive molecular dynamic simulations of shock initiation of β-cyclotetramethylene tetranitramine (β-HMX) single crystals containing nanometer-scale spherical voids. Shock induced void collapse and subsequent hot spot formation as well as chemical reaction initiation are observed which depend on the void size and impact strength. For an impact velocity of 1 km s(-1) and a void radius of 4 nm, the void collapse process includes three stages; the dominant mechanism is the convergence of upstream molecules toward the centerline and the downstream surface of the void forming flowing molecules. Hot spot formation also undergoes three stages, and the principal mechanism is kinetic energy transforming to thermal energy due to the collision of flowing molecules on the downstream surface. The high temperature of the hot spot initiates a local chemical reaction, and the breakage of the N-NO2 bond plays the key role in the initial reaction mechanism. The impact strength and void size have noticeable effects on the shock dynamical process, resulting in a variation of the predominant mechanisms leading to void collapse and hot spot formation. Larger voids or stronger shocks result in more intense hot spots and, thus, more violent chemical reactions, promoting more reaction channels and generating more reaction products in a shorter duration. The reaction products are mainly concentrated in the developed hot spot, indicating that the chemical reactivity of the hmx crystal is greatly enhanced by void collapse. The detailed information derived from this study can aid a thorough understanding of the role of void collapse in hot spot formation and the chemical reaction initiation of explosives.

  5. Environmental hot spot analysis in agricultural life-cycle assessments – three case studies

    Directory of Open Access Journals (Sweden)

    Gerhard Piringer

    2016-06-01

    Full Text Available Present-day agricultural technology is facing the challenge of limiting the environmental impacts of agricultural production – such as greenhouse gas emissions and demand for additional land – while meeting growing demands for agricultural products. Using the well-established method of life-cycle assessment (LCA, potential environmental impacts of agricultural production chains can be quantified and analyzed. This study presents three case studies of how the method can pinpoint environmental hot spots at different levels of agricultural production systems. The first case study centers on the tractor as the key source of transportation and traction in modern agriculture. A common Austrian tractor model was investigated over its life-cycle, using primary data from a manufacturer and measured load profiles for field work. In all but one of the impact categories studied, potential impacts were dominated by the operation phase of the tractor’s life-cycle (mainly due to diesel fuel consumption, with 84.4-99.6% of total impacts. The production phase (raw materials and final assembly caused between 0.4% and 12.1% of impacts, while disposal of the tractor was below 1.9% in all impact categories. The second case study shifts the focus to an entire production chain for a common biogas feedstock, maize silage. System boundaries incorporate the effect of auxiliary materials such as fertilizer and pesticides manufacturing and application. The operation of machinery in the silage production chain was found to be critical to its environmental impact. For the climate change indicator GWP100 (global warming potential, 100-year reference period, emissions from tractor operation accounted for 15 g CO2-eq per kg silage (64% of total GWP100, followed by field emissions during fertilizer (biogas digestate application with 6 g CO2-eq per kg silage (24% of total GWP100. At a larger system scale that includes a silage-fed biogas plant with electricity generated by

  6. Cell-free synthesis and multifold screening of Candida antarctica lipase B (CalB) variants after combinatorial mutagenesis of hot spots.

    Science.gov (United States)

    Park, Chang-Gil; Kwon, Min-A; Song, Jae-Kwang; Kim, Dong-Myung

    2011-01-01

    We have developed a strategy for rapid and combinatorial optimization of the hot spot residues of enzymes. After combinatorial randomization of target locations in the Candida antarctica lipase B (CalB) gene, the individual variant genes isolated in the E.coli cells were expressed in the cell-free protein synthesis system to analyze different parameters of the resulting CalB variants. The enzymatic assays for the hydrolysis of para-nitrophenyl-ester (pNP-ester) and triglyceride, synthesis of wax ester, and thermal stability of the variant enzymes were carried out simultaneously in 96-well microtiter plates. From the 1,000 variant genes tested in each assay, we were able to identify a series of the variant enzymes having markedly improved hydrolytic, synthetic activity, or thermal stability. The improved traits of the cell-free selected CalB variants were well reproduced when the corresponding genes were expressed in Pichia pastoris. Therefore, we expect that the proposed strategy of cell-free expression screening can serve as a viable option for rapid and precise tuning of enzyme molecules, not only for analytical purposes but also for industrial applications through large scale production using microbial cells transformed with variant genes selected from the cell-free expression screening.

  7. An Analytical Model for Torus Networks in the Presence of Batch Message Arrivals with Hot-spot Destinations

    Institute of Scientific and Technical Information of China (English)

    Yulei Wu; Geyong Min; Mohamed Ould-Khaoua; Hao Yin

    2009-01-01

    Interconnection networks are hardware fabrics supporting communications between individual processors in multicomputers.The low-dimensional k-ary n-cubes (or torus) with adaptive wormhole switching have attracted significant research efforts to construct high-performance interconnection networks in contemporary multi-computers.The arrival process and destination distribution of messages have great effects on network performance.With the aim of capturing the characteristics of the realistic traffic pattern and obtaining a deep understanding of the performance behaviour of interconnection networks,this paper presents an analytical model to investigate the message latency in adaptive-routed wormhole-switched torus networks where there exists hot-spot nodes and the message arrivals follow a batch arrival process.Each generated message has a given probability to be directed to the hot-spot node.The average degree of virtual channel multiplexing is computed by the GE/G/1/V queueing system with finite buffer capacity.We compare analytical results of message latency with those obtained through the simulation experiments in order to validate the accuracy of the derived model.

  8. Results and analysis of the hot-spot temperature experiment for a cable-in-conduit conductor with thick conduit

    Science.gov (United States)

    Sedlak, Kamil; Bruzzone, Pierluigi

    2015-12-01

    In the design of future DEMO fusion reactor a long time constant (∼23 s) is required for an emergency current dump in the toroidal field (TF) coils, e.g. in case of a quench detection. This requirement is driven mainly by imposing a limit on forces on mechanical structures, namely on the vacuum vessel. As a consequence, the superconducting cable-in-conduit conductors (CICC) of the TF coil have to withstand heat dissipation lasting tens of seconds at the section where the quench started. During that time, the heat will be partially absorbed by the (massive) steel conduit and electrical insulation, thus reducing the hot-spot temperature estimated strictly from the enthalpy of the strand bundle. A dedicated experiment has been set up at CRPP to investigate the radial heat propagation and the hot-spot temperature in a CICC with a 10 mm thick steel conduit and a 2 mm thick glass epoxy outer electrical insulation. The medium size, ∅ = 18 mm, NbTi CICC was powered by the operating current of up to 10 kA. The temperature profile was monitored by 10 temperature sensors. The current dump conditions, namely the decay time constant and the quench detection delay, were varied. The experimental results show that the thick conduit significantly contributes to the overall enthalpy balance, and consequently reduces the amount of copper required for the quench protection in superconducting cables for fusion reactors.

  9. Environmental health risk assessment of dioxin exposure through foods in a dioxin hot spot-Bien Hoa City, Vietnam.

    Science.gov (United States)

    Tuyet-Hanh, Tran Thi; Vu-Anh, Le; Ngoc-Bich, Nguyen; Tenkate, Thomas

    2010-05-01

    This study used the Australian Environmental Health Risk Assessment Framework to assess the human health risk of dioxin exposure through foods for local residents in two wards of Bien Hoa City, Vietnam. These wards are known hot-spots for dioxin and a range of stakeholders from central government to local levels were involved in this process. Publications on dioxin characteristics and toxicity were reviewed and dioxin concentrations in local soil, mud, foods, milk and blood samples were used as data for this risk assessment. A food frequency survey of 400 randomly selected households in these wards was conducted to provide data for exposure assessment. Results showed that local residents who had consumed locally cultivated foods, especially fresh water fish and bottom-feeding fish, free-ranging chicken, duck, and beef were at a very high risk, with their daily dioxin intake far exceeding the tolerable daily intake recommended by the WHO. Based on the results of this assessment, a multifaceted risk management program was developed and has been recognized as the first public health program ever to have been implemented in Vietnam to reduce the risks of dioxin exposure at dioxin hot-spots.

  10. Hydrodynamic Mixing of Ablator Material into the Compressed Fuel and Hot Spot of Direct-Drive DT Cryogenic Implosions

    Science.gov (United States)

    Regan, S. P.; Goncharov, V. N.; Epstein, R.; Betti, R.; Bonino, M. J.; Cao, D.; Collins, T. J. B.; Campbell, E. M.; Forrest, C. J.; Glebov, V. Yu.; Harding, D. R.; Marozas, J. A.; Marshall, F. J.; McKenty, P. W.; Sangster, T. C.; Stoeckl, C.; Luo, R. W.; Schoff, M. E.; Farrell, M.

    2016-10-01

    Hydrodynamic mixing of ablator material into the compressed fuel and hot spot of direct-drive DT cryogenic implosions is diagnosed using time-integrated, spatially resolved xray spectroscopy. The laser drive ablates most of the 8- μm-thick CH ablator, which is doped with trace amounts of Ge ( 0.5 at.) and surrounds the cryogenic DT layer. A small fraction of the ablator material is mixed into the compressed shell and the hot spot by the ablation-front Rayleigh-Taylor hydrodynamic instability seeded by laser imprint, the target mounting stalk, and surface debris. The amount of mix mass inferred from spectroscopic analysis of the Ge K-shell emission will be presented. This material is based upon work supported by the Department Of Energy National Nuclear Security Administration under Award Number DE-NA0001944. Part of this work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.

  11. Identification of Interaction Hot Spots in Structures of Drug Targets on the Basis of Three-Dimensional Activity Cliff Information.

    Science.gov (United States)

    Furtmann, Norbert; Hu, Ye; Gütschow, Michael; Bajorath, Jürgen

    2015-12-01

    Activity cliffs are defined as pairs or groups of structurally similar or analogous compounds that share the same specific activity but have large differences in potency. Although activity cliffs are mostly studied in medicinal chemistry at the level of molecular graphs, they can also be assessed by comparing compound binding modes. If such three-dimensional activity cliffs (3D-cliffs) are studied on the basis of X-ray complex structures, experimental ligand-target interaction details can be taken into account. Rapid growth in the number of 3D-cliffs that can be derived from X-ray complex structures has made it possible to identify targets for which a substantial body of 3D-cliff information is available. Activity cliffs are typically studied to identify structure-activity relationship determinants and aid in compound optimization. However, 3D-cliff information can also be used to search for interaction hot spots and key residues, as reported herein. For six of seven drug targets for which more than 20 3D-cliffs were available, series of 3D-cliffs were identified that were consistently involved in interactions with different hot spots. These 3D-cliffs often encoded chemical modifications resulting in interactions that were characteristic of highly potent compounds but absent in weakly potent ones, thus providing information for structure-based design.

  12. Three-Dimensional Analysis of the Hot-Spot Fuel Temperature in Pebble Bed and Prismatic Modular Reactors

    Energy Technology Data Exchange (ETDEWEB)

    In, W. K.; Lee, S. W.; Lim, H. S.; Lee, W. J. [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    2006-07-01

    High temperature gas-cooled reactors(HTGR) have been reviewed as potential sources for future energy needs, particularly for a hydrogen production. Among the HTGRs, the pebble bed reactor(PBR) and a prismatic modular reactor(PMR) are considered as the nuclear heat source in Korea's nuclear hydrogen development and demonstration project. PBR uses coated fuel particles embedded in spherical graphite fuel pebbles. The fuel pebbles flow down through the core during an operation. PMR uses graphite fuel blocks which contain cylindrical fuel compacts consisting of the fuel particles. The fuel blocks also contain coolant passages and locations for absorber and control material. The maximum fuel temperature in the core hot spot is one of the important design parameters for both a PBR and a PMR. The objective of this study is to predict the hot-spot fuel temperature distributions in a PBR and a PMR at a steady state. The computational fluid dynamics(CFD) code, CFX-10 is used to perform the three-dimensional analysis. The latest design data was used here based on the reference reactor designs, PBMR400 and GTMHR60.

  13. Identification of a Non-Gatekeeper Hot Spot for Drug-Resistant Mutations in mTOR Kinase

    Directory of Open Access Journals (Sweden)

    Tzung-Ju Wu

    2015-04-01

    Full Text Available Protein kinases are therapeutic targets for human cancer. However, “gatekeeper” mutations in tyrosine kinases cause acquired clinical resistance, limiting long-term treatment benefits. mTOR is a key cancer driver and drug target. Numerous small-molecule mTOR kinase inhibitors have been developed, with some already in human clinical trials. Given our clinical experience with targeted therapeutics, acquired drug resistance in mTOR is thought likely, but not yet documented. Herein, we describe identification of a hot spot (L2185 for drug-resistant mutations, which is distinct from the gatekeeper site, and a chemical scaffold refractory to drug-resistant mutations. We also provide new insights into mTOR kinase structure and function. The hot spot mutations are potentially useful as surrogate biomarkers for acquired drug resistance in ongoing clinical trials and future treatments and for the design of the next generation of mTOR-targeted drugs. Our study provides a foundation for further research into mTOR kinase function and targeting.

  14. Effects of basal-plane thermal conductivity and interface thermal conductance on the hot spot temperature in graphene electronic devices

    Science.gov (United States)

    Choi, David; Poudel, Nirakar; Cronin, Stephen B.; Shi, Li

    2017-02-01

    Electrostatic force microscopy and scanning thermal microscopy are employed to investigate the electric transport and localized heating around defects introduced during transfer of graphene grown by chemical vapor deposition to an oxidized Si substrate. Numerical and analytical models are developed to explain the results based on the reported basal-plane thermal conductivity, κ, and interfacial thermal conductance, G, of graphene and to investigate their effects on the peak temperature. Irrespective of the κ values, increasing G beyond 4 × 107 W m-2 K-1 can reduce the peak temperature effectively for graphene devices made on sub-10 nm thick gate dielectric, but not for the measured device made on 300-nm-thick oxide dielectric, which yields a cross-plane thermal conductance (Gox) much smaller than the typical G of graphene. In contrast, for typical G values reported for graphene, increasing κ from 300 W m-1 K-1 toward 3000 W m-1 K-1 is effective in reducing the hot spot temperature for the 300-nm-thick oxide devices but not for the sub-10 nm gate dielectric case, because the heat spreading length (l) can be appreciably increased relative to the micron-scale localized heat generation spot size (r0) only when the oxide layer is sufficiently thick. As such, enhancement of κ increases the vertical heat transfer area above the gate dielectric only for the thick oxide case. In all cases considered, the hot spot temperature is sensitive to varying G and κ only when the G/Gox ratio and r0/l ratio are below about 5, respectively.

  15. Demonstration of Fuel Hot-Spot Pressure in Excess of 50 Gbar for Direct-Drive, Layered Deuterium-Tritium Implosions on OMEGA

    Science.gov (United States)

    Regan, S. P.; Goncharov, V. N.; Igumenshchev, I. V.; Sangster, T. C.; Betti, R.; Bose, A.; Boehly, T. R.; Bonino, M. J.; Campbell, E. M.; Cao, D.; Collins, T. J. B.; Craxton, R. S.; Davis, A. K.; Delettrez, J. A.; Edgell, D. H.; Epstein, R.; Forrest, C. J.; Frenje, J. A.; Froula, D. H.; Gatu Johnson, M.; Glebov, V. Yu.; Harding, D. R.; Hohenberger, M.; Hu, S. X.; Jacobs-Perkins, D.; Janezic, R.; Karasik, M.; Keck, R. L.; Kelly, J. H.; Kessler, T. J.; Knauer, J. P.; Kosc, T. Z.; Loucks, S. J.; Marozas, J. A.; Marshall, F. J.; McCrory, R. L.; McKenty, P. W.; Meyerhofer, D. D.; Michel, D. T.; Myatt, J. F.; Obenschain, S. P.; Petrasso, R. D.; Radha, P. B.; Rice, B.; Rosenberg, M. J.; Schmitt, A. J.; Schmitt, M. J.; Seka, W.; Shmayda, W. T.; Shoup, M. J.; Shvydky, A.; Skupsky, S.; Solodov, A. A.; Stoeckl, C.; Theobald, W.; Ulreich, J.; Wittman, M. D.; Woo, K. M.; Yaakobi, B.; Zuegel, J. D.

    2016-07-01

    A record fuel hot-spot pressure Phs=56 ±7 Gbar was inferred from x-ray and nuclear diagnostics for direct-drive inertial confinement fusion cryogenic, layered deuterium-tritium implosions on the 60-beam, 30-kJ, 351-nm OMEGA Laser System. When hydrodynamically scaled to the energy of the National Ignition Facility, these implosions achieved a Lawson parameter ˜60 % of the value required for ignition [A. Bose et al., Phys. Rev. E 93, LM15119ER (2016)], similar to indirect-drive implosions [R. Betti et al., Phys. Rev. Lett. 114, 255003 (2015)], and nearly half of the direct-drive ignition-threshold pressure. Relative to symmetric, one-dimensional simulations, the inferred hot-spot pressure is approximately 40% lower. Three-dimensional simulations suggest that low-mode distortion of the hot spot seeded by laser-drive nonuniformity and target-positioning error reduces target performance.

  16. Hot moments and hot spots in hyporheic nutrient transformation - To what degree does small-scale variability control stream-reach attenuation potential?

    Science.gov (United States)

    Krause, S.; Blume, T.; Binley, A.; Heathwaite, L.; Cassidy, N. J.; Munz, M.; Tecklenburg, C.; Kaeser, D.

    2011-12-01

    Concentrations of nutrients and contaminants in up-welling groundwater can significantly change along the passage through highly heterogeneous streambed sediments with substantial implications for the quality of connected surface water bodies. This study presents investigations into the physical drivers and chemical controls of nutrient transport and transformation at the aquifer-river interfaces of two upland and lowland UK rivers. It combines the application of in-stream geophysical exploration techniques, multi-level mini-piezometer networks, active and passive heat tracing methods (including fibre-optic distributed temperature sensing - FO-DTS) for identifying hyporheic exchange fluxes and residence time distributions with multi-scale approaches of hyporheic pore water sampling and reactive tracers for analysing the patterns of streambed redox conditions and chemical transformation rates. The analysis of hyporheic pore water from nested multi-level mini piezometers and passive gel probe samplers revealed significant spatial variability in streambed redox conditions and concentration changes of nitrogen species, dissolved oxygen and bioavailable organic carbon. Hot spots of increased nitrate attenuation were identified beneath semi-confining peat lenses in the streambed of the investigated lowland river. The intensity of concentration changes underneath the confining peat pockets correlated with the state of anoxia in the pore water as well as the supply of organic carbon and hyporheic residence times. In contrast, at locations where flow inhibiting peat layers were absent or disrupted - fast exchange between aquifer and river caused a break through of nitrate without significant concentration changes along the hyporheic flow path. Fibre-optic distributed temperature sensor networks and streambed electric resistivity tomography were applied for identifying exchange flow patterns between groundwater and surface water in dependency of streambed structural

  17. Hot-spot model for accretion disc variability as random process

    CERN Document Server

    Pechacek, T; Czerny, B

    2008-01-01

    Theory of random processes provides an attractive mathematical tool to describe the fluctuating signal from accreting sources, such as active galactic nuclei and Galactic black holes observed in X-rays. These objects exhibit featureless variability on different timescales, probably originating from an accretion disc. We study the basic features of the power spectra in terms of a general framework, which permits semi-analytical determination of the power spectral density (PSD) of the resulting light curve. We consider the expected signal generated by an ensemble of spots randomly created on the accretion disc surface. Spot generation is governed by Poisson or by Hawkes processes. We include general relativity effects shaping the signal on its propagation to a distant observer. We analyse the PSD of a spotted disc light curve and show the accuracy of our semi-analytical approach by comparing the obtained PSD with the results of Monte Carlo simulations. The asymptotic slopes of PSD are 0 at low frequencies and t...

  18. Channeling at the base of the lithosphere during the lateral flow of plume material beneath flow line hot spots

    Science.gov (United States)

    Sleep, Norman H.

    2008-08-01

    Chains of volcanic edifices lie along flow lines between plume-fed hot spots and the thin lithosphere at ridge axes. Discovery and Euterpe/Musicians Seamounts are two examples. An attractive hypothesis is that buoyant plume material flows along the base of the lithosphere perpendicular to isochrons. The plume material may conceivably flow in a broad front or flow within channels convectively eroded into the base to the lithosphere. A necessary but not sufficient condition for convective channeling is that the expected stagnant-lid heat flow for the maximum temperature of the plume material is comparable to the half-space surface heat flow of the oceanic lithosphere. Two-dimensional and three-dimensional numerical calculations confirm this inference. A second criterion for significant convective erosion is that it needs to occur before the plume material thins by lateral spreading. Scaling relationships indicate spreading and convection are closely related. Mathematically, the Nusselt number (ratio of convective to conductive heat flow in the plume material) scales with the flux (volume per time per length of flow front) of the plume material. A blob of unconfined plume material thus spreads before the lithosphere thins much and evolves to a slowly spreading and slowly convecting warm region in equilibrium with conduction into the base of the overlying lithosphere. Three-dimensional calculations illustrate this long-lasting (and hence observable) state of plume material away from its plume source. A different flow domain occurs around a stationary hot plume that continuously supplies hot material. The plume convectively erodes the overlying lithosphere, trapping the plume material near its orifice. The region of lithosphere underlain by plume material grows toward the ridge axis and laterally by convective thinning of the lithosphere at its edges. The hottest plume material channels along flow lines. Geologically, the regions of lithosphere underlain by either warm

  19. Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats.

    Directory of Open Access Journals (Sweden)

    Simone Dealtry

    Full Text Available IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC- DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots" of plasmids potentially carrying catabolic genes.

  20. Professional- Amateur Astronomer Partnerships in Scientific Research: The Re-emergence of Jupiter's 5-Micron Hot Spots

    Science.gov (United States)

    Yanamandra-Fisher, P. A.

    2012-12-01

    The night sky, with all its delights and mysteries, enthrall professional and amateur astronomers alike. The discrete data sets acquired by professional astronomers via their approved observing programs at various national facilities are supplemented by the nearly daily observations of the same celestial object by amateur astronomers around the world. The emerging partnerships between professional and dedicated amateur astronomers rely on creating a niche for long timeline of multispectral remote sensing. "Citizen Astronomy" can be thought of as the paradigm shift transforming the nature of observational astronomy. In the past decade, it is the collective observations and their analyses by the ever-increasing global network of amateur astronomers that has discovered interesting phenomena and provided the reference backdrop for observations by ground-based professional astronomers and spacecraft missions. We shall present results from our collaborations to observe the recent global upheaval on Jupiter for the past five years and illustrate the strong synergy between the two groups. Global upheavals on Jupiter involve changes in the albedo of entire axisymmetric regions, lasting several years, with the last two occurring in 1989 and 2006. Against this backdrop of planetary-scale changes, discrete features such as the Great Red Spot (GRS), and other vortices exhibit changes on shorter spatial- and time-scales. One set of features we are currently tracking is the variability of the discrete equatorial 5-μm hot spots, semi-evenly spaced in longitude and confined to a narrow latitude band centered at 6.5°N (southern edge of the North Equatorial Belt, NEB), abundant in Voyager images (1980-1981). Tantalizingly similar patterns were observed in the visible (bright plumes and blue-gray regions), where reflectivity in the red is anti-correlated with 5-μm thermal radiance. During the recent NEB fade (2011 - early 2012), however, these otherwise ubiquitous features were

  1. Unusual seismic activity in 2011 and 2013 at the submarine volcano Rocard, Society hot spot (French Polynesia)

    Science.gov (United States)

    Talandier, Jacques; Hyvernaud, Olivier; Maury, René C.

    2016-05-01

    We analyze two seismic events that occurred on 27 May 2011 and 29 April 2013 at the Rocard submarine volcano which overlies the Society hot spot. The Polynesian Seismic Network recorded for the first time unusual associated short- and long-period signals, with perfectly monochromatic (0.0589 Hz) Rayleigh wave trains of long period and duration. None of the numerous observations of long-period (10-30 s) signals previously associated with volcanic activity in Japan, Italy, Mexico, Indonesia, Antarctica, and the Hawaiian Islands have the characteristics we observed at Rocard. We propose a tentative model for these unusual and rather enigmatic signals, in which the movement of lava excited the resonance of a shallow open conduit under a high hydrostatic pressure of ~400 bars.

  2. Particle Accelerators in the Hot Spots of the Radio Galaxy 3C445 Imaged With the VLT

    CERN Document Server

    Prieto, M A; Mack, K H; Brunetti, Gianfranco; Mack, Karl-Heinz

    2002-01-01

    Hot spots (HSs) are regions of enhanced radio emission produced by supersonic jets at the tip of the radio lobes of powerful radiosources. Obtained with the Very Large Telescope (VLT), images of the HSs in the radio galaxy 3C445 show bright knots embedded in diffuse optical emission distributed along the post shock region created by the impact of the jet into the intergalactic medium. The observations reported here confirm that relativistic electrons are accelerated by Fermi-I acceleration processes in HSs. Furthermore, both the diffuse emission tracing the rims of the front shock and the multiple knots demonstrate the presence of additional continuous re-acceleration processes of electrons (Fermi-II).

  3. Turbulence influence on urban air pollution in a hot spot in Madrid: comparison of winter and summer field campaigns

    Science.gov (United States)

    Yagüe, Carlos; Román-Cascón, Carlos; Sastre, Mariano; Maqueda, Gregorio; Arrillaga, Jon A.; Artiñano, Begoña; Díaz-Ramiro, Elías; Gómez-Moreno, Francisco J.; Borge, Rafael; Narros, Adolfo; Pérez, Javier; Quaassdorff, Christina

    2017-04-01

    Air pollution is a major problem in the city of Madrid during weak synoptic forcing, since the presence of atmospheric stability conditions often develops night surface-based thermal inversions and subsidence inversions during daytime for several consecutive days, reaching high levels of NOx and Particulate Matter (PM) concentration. In this context, the TECNAIRE-CM (Innovative technologies for the assessment and improvement of urban air quality) research project has developed two field campaigns along 2015 (winter and summer) in a hot spot in the city of Madrid (Fernández Ladreda square). This hot spot includes one important intersection of different streets and also the start of the A42 motorway, which crosses down the square through a tunnel of about 150 m length. Besides, the location has numerous traffic lights and a lot of pedestrians walking in the vicinity. In addition to direct measurements related to air quality, data from different meteorological variables were recorded in order to characterize the atmospheric conditions. Moreover, two sonic anemometers where deployed to carry out a micrometeorological assessment of physical processes that take place in the urban atmospheric surface layer (TKE, friction velocity and sensible heat flux were evaluated). The evolution of the turbulence will be analyzed and compared for both campaigns (winter and summer), searching for the key seasonal differences as well as the importance of the different scales influencing the diffusion of pollutants (from multi resolution flux decomposition -MRFD- analysis). Specific case studies corresponding to high levels of pollution will be studied in detailed, to understand local pollution dynamics under the influence of both high traffic density and low turbulence situations. This work has been financed by Madrid Regional Research Plan through TECNAIRE (P2013/MAE-2972).

  4. Management adaptation of invertebrate fisheries to an extreme marine heat wave event at a global warming hot spot.

    Science.gov (United States)

    Caputi, Nick; Kangas, Mervi; Denham, Ainslie; Feng, Ming; Pearce, Alan; Hetzel, Yasha; Chandrapavan, Arani

    2016-06-01

    An extreme marine heat wave which affected 2000 km of the midwest coast of Australia occurred in the 2010/11 austral summer, with sea-surface temperature (SST) anomalies of 2-5°C above normal climatology. The heat wave was influenced by a strong Leeuwin Current during an extreme La Niña event at a global warming hot spot in the Indian Ocean. This event had a significant effect on the marine ecosystem with changes to seagrass/algae and coral habitats, as well as fish kills and southern extension of the range of some tropical species. The effect has been exacerbated by above-average SST in the following two summers, 2011/12 and 2012/13. This study examined the major impact the event had on invertebrate fisheries and the management adaption applied. A 99% mortality of Roei abalone (Haliotis roei) and major reductions in recruitment of scallops (Amusium balloti), king (Penaeus latisulcatus) and tiger (P. esculentus) prawns, and blue swimmer crabs were detected with management adapting with effort reductions or spatial/temporal closures to protect the spawning stock and restocking being evaluated. This study illustrates that fisheries management under extreme temperature events requires an early identification of temperature hot spots, early detection of abundance changes (preferably using pre-recruit surveys), and flexible harvest strategies which allow a quick response to minimize the effect of heavy fishing on poor recruitment to enable protection of the spawning stock. This has required researchers, managers, and industry to adapt to fish stocks affected by an extreme environmental event that may become more frequent due to climate change.

  5. Hot prospect for new gene amplifier

    Energy Technology Data Exchange (ETDEWEB)

    1991-11-29

    Molecular biologist Francis Barany is investigating one of the hottest areas in biotechnology: a gene-amplification technique called ligase chain reaction, or LCR. Already scientists have used LCR to detect the tiny mutation that causes sickle cell anemia and have adapted it to screen for a handful of other genetic diseases simultaneously - in a single test-tube. Some experts, in fact, are predicting that LCR will supplement the polymerase chain reaction (PCR), and in some cases even supplant it. LCR could revolutionize DNA diagnostics just as PCR transformed basic molecular biology following its introduction 6 years ago. With its ease of automation and ability to produce useful quantitative results, LCR could become a major player in the rapidly growing market for DNA diagnostics. LCR, like PCR, uses snippets of nucleic acid, or oligonucleotides, that anneal to a specific, complementary sequence on the target DNA to be amplified. But where PCR uses oligos that bracket the stretch to be amplified, LCR uses pairs of oligos that completely cover the target sequence.

  6. A Hot Spot and Mass Transfer of the Algol-type Binary System WZ Crv

    CERN Document Server

    Virnina, Natalia A; Mogorean, Maxim V

    2011-01-01

    We present the results of two color VR observation of the Algol-type binary system WZ Crv (12h44m15.19s, -21d25m35.4s) which were obtained using the remotely controlled telescope TOA-150 of Tzec Maun Observatory. We determined the moments of individual minima, the orbital period and its derivative, the initial epoch, color indices V-R and temperature estimates of the components. Also we noticed that the phase curve is asymmetric: the second maximum is higher than the first one. It indicates that there is a spot in the photosphere of one of the stars in this system.

  7. Infrared radiation emitted due to scanning of a hot spot as a probe of hidden defects

    Science.gov (United States)

    Woźny, Mariusz; Maś, Kinga; Prokhorenko, Serhiy; Ploch, Dariusz; Sheregii, E. M.

    2016-05-01

    Specially created subsurface defects in a sample are detected using a high resolution infrared camera FLIR SC7000. A scanning hot air (about 110 °C) nozzle is applied to introduce additional energy in a researched sample. The hidden defect has an increased temperature in comparison with the surrounding area that is a result of changed emissivity and thermal diffusivity. The suggested method is compared with pulse thermography which uses a xenon lamp for excitation.

  8. Liquid Metal Embrittlement in Resistance Spot Welding and Hot Tensile Tests of Surface-refined TWIP Steels

    Science.gov (United States)

    Barthelmie, J.; Schram, A.; Wesling, V.

    2016-03-01

    Automotive industry strives to reduce vehicle weight and therefore fuel consumption and carbon dioxide emissions. Especially in the auto body, material light weight construction is practiced, but the occupant safety must be ensured. These requirements demand high-strength steels with good forming and crash characteristics. Such an approach is the use of high- manganese-content TWIP steels, which achieve strengths of around 1,000 MPa and fracture strains of more than 60%. Welding surface-refined TWIP steels reduces their elongation at break and produces cracks due to the contact with liquid metal and the subsequent liquid metal embrittlement (LME). The results of resistance spot welds of mixed joints of high-manganese- content steel in combination with micro-alloyed ferritic steel and hot tensile tests are presented. The influence of different welding parameters on the sensitivity to liquid metal embrittlement is investigated by means of spot welding. In a high temperature tensile testing machine, the influence of different parameters is determined regardless of the welding process. Defined strains just below or above the yield point, and at 25% of elongation at break, show the correlation between the applied strain and liquid metal crack initiation. Due to the possibility to carry out tensile tests on a wide range of temperatures, dependencies of different temperatures of the zinc coating to the steel can be identified. Furthermore, the attack time of the zinc on the base material is investigated by defined heating periods.

  9. Exploring the spatial variation in quality-adjusted rental prices and identifying hot spots in Berlin’s residential property market

    DEFF Research Database (Denmark)

    Meulen, Philipp an de; Mitze, Timo Friedel

    2014-01-01

    of quality-adjusted rental prices for apartments across the residential locations within the city of Berlin. The resulting pattern of ‘residual’ rental prices with a growing concentration of hot spots in central districts of Berlin can be interpreted as the tenants’ valorization of apartments in geographic...

  10. WLAN Hot Spot services for the automotive and oil industries :a business analysis Or : "Refuel the car with petrol and information, both ways at the gas station"

    NARCIS (Netherlands)

    L-F. Pau (Louis-François); M.H.P. Oremus

    2003-01-01

    textabstractWhile you refuel for gas ,why not refuel for information or download vehicle data ? This paper analyzes in extensive detail the user segmentation by vehicle usage , service offering , and full business models from WLAN hot spot services delivered to vehicles (private, professional ,

  11. WLAN Hot Spot services for the automotive and oil industries :a business analysis Or : "Refuel the car with petrol and information, both ways at the gas station"

    NARCIS (Netherlands)

    L-F. Pau (Louis-François); M.H.P. Oremus

    2003-01-01

    textabstractWhile you refuel for gas ,why not refuel for information or download vehicle data ? This paper analyzes in extensive detail the user segmentation by vehicle usage , service offering , and full business models from WLAN hot spot services delivered to vehicles (private, professional , publ

  12. Multidisciplinary approach: mapping potential TB transmission 'hot spots' in high burden communities

    CSIR Research Space (South Africa)

    Murray, EJ

    2009-06-01

    Full Text Available potential TB transmission ‘hot spots’ in high burden communities. EJ Murray 1, 2 , BJ Marais 1, 2 , G Mans3, N Beyers1, H Ayles4,5, P Godfrey-Faussett4, S Wallman6, V Bond5,7. 1 Desmond Tutu TB Centre, Stellenbosch University, South Africa; 2...Ukwanda Centre for Rural Health, Stellenbosch University, South Africa; 3Unit for Religion and Development Research, Stellenbosch University, South Africa; 4Clinical Research Unit, Department of Infectious and Tropical Disease, London School of Hygiene...

  13. Transport properties of a charged hot spot in an external electromagnetic field

    Energy Technology Data Exchange (ETDEWEB)

    Bondarenko, S., E-mail: sergeyb@ariel.ac.il; Komoshvili, K.; Prygarin, A.

    2016-06-15

    We investigate adiabatic expansion of a charged and rotating fluid element consisting of weakly interacting particles, which is initially perturbed by an external electromagnetic field. A framework for the perturbative calculation of the non-equilibrium distribution function of this fluid volume is considered and the distribution function is calculated to the first order in the perturbative expansion. This distribution function, which describes the evolution of the element with constant entropy, allows to calculate momentum flux tensor and viscosity coefficients of the expanding system. We show, that these viscosity coefficients depend on the initial angular velocity of the spot and on the strength of its initial perturbation by the external field. Obtained results are applied to the phenomenology of the viscosity to the entropy ratio calculated in lattice models.

  14. A Module Located at a Chromosomal Integration Hot Spot Is Responsible for the Multidrug Resistance of a Reference Strain from Escherichia coli Clonal Group A▿ †

    Science.gov (United States)

    Lescat, Mathilde; Calteau, Alexandra; Hoede, Claire; Barbe, Valérie; Touchon, Marie; Rocha, Eduardo; Tenaillon, Olivier; Médigue, Claudine; Johnson, James R.; Denamur, Erick

    2009-01-01

    Escherichia coli clonal group A (CGA) commonly exhibits a distinctive multidrug antimicrobial resistance phenotype—i.e., resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, tetracycline, and trimethoprim (ACSSuTTp)—and has accounted for up to 50% of trimethoprim-sulfamethoxazole-resistant E. coli urinary tract infections in some locales. Annotation of the whole-genome sequencing of UMN026, a reference CGA strain, clarified the genetic basis for this strain's ACSSuTTp antimicrobial resistance phenotype. Most of the responsible genes were clustered in a unique 23-kbp chromosomal region, designated the genomic resistance module (GRM), which occurred within a 105-kbp genomic island situated at the leuX tRNA. The GRM is characterized by numerous remnants of mobilization and rearrangement events suggesting multiple horizontal transfers. Additionally, comparative genomic analysis of the leuX tRNA genomic island in 14 sequenced E. coli genomes showed that this region is a hot spot of integration, with the presence/absence of specific subregions being uncorrelated with either the phylogenetic group or the pathotype. Our data illustrate the importance of whole-genome sequencing in the detection of genetic elements involved in antimicrobial resistance. Additionally, this is the first documentation of the blaTEM and dhfrVII genes in a chromosomal location in E. coli strains. PMID:19364861

  15. A spatial analysis of population dynamics and climate change in Africa: potential vulnerability hot spots emerge where precipitation declines and demographic pressures coincide

    Science.gov (United States)

    López-Carr, David; Pricope, Narcisa G.; Aukema, Juliann E.; Jankowska, Marta M.; Funk, Christopher C.; Husak, Gregory J.; Michaelsen, Joel C.

    2014-01-01

    We present an integrative measure of exposure and sensitivity components of vulnerability to climatic and demographic change for the African continent in order to identify “hot spots” of high potential population vulnerability. Getis-Ord Gi* spatial clustering analyses reveal statistically significant locations of spatio-temporal precipitation decline coinciding with high population density and increase. Statistically significant areas are evident, particularly across central, southern, and eastern Africa. The highly populated Lake Victoria basin emerges as a particularly salient hot spot. People located in the regions highlighted in this analysis suffer exceptionally high exposure to negative climate change impacts (as populations increase on lands with decreasing rainfall). Results may help inform further hot spot mapping and related research on demographic vulnerabilities to climate change. Results may also inform more suitable geographical targeting of policy interventions across the continent.

  16. Evolutionary dynamics of a mitochondrial rearrangement "hot spot" in the Hymenoptera.

    Science.gov (United States)

    Dowton, M; Austin, A D

    1999-02-01

    The arrangement of tRNA genes at the junction of the cytochrome oxidase II and ATPase 8 genes was examined across a broad range of Hymenoptera. Seven distinct arrangements of tRNA genes were identified among a group of wasps that have diverged over the last 180 Myr (suborder Apocrita); many of the rearrangements represent evolutionarily independent events. Approximately equal proportions of local rearrangements, inversions, and translocations were observed, in contrast to vertebrate mitochondria, in which local rearrangements predominate. Surprisingly, homoplasy was evident among certain types of rearrangement; a reversal of the plesiomorphic gene order has arisen on three separate occasions in the Insecta, while the tRNA(H) gene has been translocated to this locus on two separate occasions. Phylogenetic analysis indicates that this gene translocation is real and is not an artifactual translocation resulting from the duplication of a resident tRNA gene followed by mutation of the anticodon. The nature of the intergenic sequences surrounding this region does not indicate that it should be especially prone to rearrangement; it does not generally have the tandem or inverted repeats that might facilitate this plasticity. Intriguingly, these findings are consistent with the view that during the evolution of the Hymenoptera, rearrangements increased at the same time that the rate of point mutations and compositional bias also increased. This association may direct investigations into mitochondrial genome plasticity in other invertebrate lineages.

  17. Selecting optimal parallel microchannel configurations for active hot spot mitigation of multicore microprocessors in real time

    CERN Document Server

    Maganti, Lakshmi Sirisha; Sundararajan, T; Das, Sarit K

    2016-01-01

    Design of effective micro cooling systems to address the challenges of ever increasing heat flux from microdevices requires deep examination of real time problems and has been tackled in depth. The most common and apparently misleading assumption while designing micro cooling systems is that the heat flux generated by the device is uniform, but the reality is far from this. Detailed simulations have been performed by considering non uniform heat load employing the configurations U, I, Z for parallel microchannel systems with water and nanofluids as the coolants. An Intel Core i7 4770 3.40 GHz quad core processor has been mimicked using heat load data retrieved from a real microprocessor with non-uniform core activity. The study clearly demonstrates that there is a non-uniform thermal load induced temperature maldistribution along with the already existent flow maldistribution induced temperature maldistribution. The suitable configuration(s) for maximum possible overall heat removal for a hot zone while maxim...

  18. Large-Scale Hot Spot Engineering for Quantitative SERS at the Single-Molecule Scale.

    Science.gov (United States)

    Chen, Hung-Ying; Lin, Meng-Hsien; Wang, Chun-Yuan; Chang, Yu-Ming; Gwo, Shangjr

    2015-10-28

    Quantitative surface enhanced Raman spectroscopy (SERS) requires precise control of Raman enhancement factor and detection uniformity across the SERS substrate. Here, we show that alkanethiolate ligand-regulated silver (Ag) nanoparticle films can be used to achieve quantitative SERS measurements down to the single-molecule level. The two-dimensional hexagonal close-packed superlattices of Ag nanoparticles formed in these films allow for SERS detection over a large area with excellent uniformity and high Raman enhancement factor. In particular, the SERS signal from the thiolate ligands on Ag nanoparticle surfaces can be utilized as a stable internal calibration standard for reproducible quantitative measurements. We demonstrate the capability of quantitative SERS by measuring the areal densities of crystal violet molecules embedded in an ultrathin spin-on-glass detection "hot zone", which is a planar and uniformly enhanced region several nanometers above the Ag nanoparticles. The Raman measurement results exhibit a linear response over a wide dynamic range of analyte concentration.

  19. Differential Expression of R-genes to Associate Leaf Spot Resistance in Cultivated Peanut

    Science.gov (United States)

    Breeding for acceptable levels of Early (ELS) or Late Leaf Spot (LLS) resistance in cultivated peanut has been elusive due to extreme variability of plant response in the field and the proper combinations of resistance (R)-genes in any particular peanut line. R-genes have been shown to be involved ...

  20. Juan Fernández Ridge (Nazca Plate): petrology and thermochronology of a rejuvenated hot spot trail

    Science.gov (United States)

    Reyes, J.; Lara, L. E.

    2012-04-01

    The Juan Fernández Ridge on the oceanic Nazca plate is thought to be a classic hot spot trail because of the apparent westward rejuvenation of the eruptive ages. However, geochronological data is still scarce and there are a few constrains to support this hypothesis like the ca. 9 Ma Ar-Ar age of the O'Higgins seamount (115 km from the Chile-Perú trench), some published K-Ar ages of ca. 3-4 Ma in Robinson Crusoe island (580 km from the trench) and ca. 1 Ma in Alejandro Selkirk (180 km further west). New reconnaissance K-Ar ages and specially the ongoing Ar-Ar dating effort in Robinson Crusoe define a ca. 1-4 Ma time span, which partially overlap with the age of Alejandro Selkirk, breaking the expected age progression given that the Nazca plate moves eastwards at ca. 6-8 cm/yr. In addition, new geological mapping shows a sharp unconformity between the older (ca. 4 Ma), strongly altered sequences and the more recent (ca. 1 Ma), post-erosional volcanic piles, where the proximal facies are still preserved. Petrological evidence also supports this evolution pattern. In fact, the partially altered older sequence is tholeiitic (Ba/Yb=12.70; La/Yb=8.12; Ba/Y=6.51; Ba/Zr=0.89). The shield stage (ca. 1-3 Ma) is transicional from tholeiitic to alkaline (Ba/Yb=18.07-8.32; La/Yb=4.59-9.84; Ba/Y=4.24-8.18; Ba/Zr=0.73-1.09) and the younger (ca. 1 Ma) is mostly alkaline (Ba/Yb=38.15; La/Yb=15.66; Ba/Y=20.27; Ba/Zr=2.31). A fixed deep-mantle plume origin for Pacific hot spots has been widely debated and concurrent phenomena arose as a possible explanation for non-linear age progressions and/or long-lived volcanic activity. In fact, intraplate regional tectonics, plume displacement, and mantle heterogeneities could be the main factor of the ridge architecture or the mask for a first-order linear trend. An ongoing mapping and dating effort is aimed to understand the evolution of the Juan Fernández Ridge, testing the main hypothesis. This research is supported by FONDECYT Project

  1. Demonstration of 55 +/- 7-Gbar Hot-Spot Pressure in Direct-Drive Layered DT Cryogenic Implosions on OMEGA

    Science.gov (United States)

    Regan, S. P.

    2015-11-01

    Direct-drive ignition target designs for the National Ignition Facility (NIF) require hot-spot pressures in excess of 100 Gbar. Only one-third of the required pressure was inferred in earlier experimental campaigns conducted on the 60-beam, 30-kJ, 351-nm OMEGA laser with direct-drive implosions of layered DT cryogenic targets. Laser and target improvements were implemented on OMEGA to increase the stagnation pressure, including a set of phase plates to increase the laser irradiation uniformity on target and a purified fuel with isotope composition reaching a 50:50 DT ratio. Diagnostic improvements were made for a neutron burnwidth measurement with a 40-ps impulse response and a 16-channel Kirkpatrick-Baez microscope to measure gated (30-ps) x-ray images of the core near peak compression with 6- μm resolution. The inferred volume-averaged, peak pressure in the current campaign almost doubled to 55 +/- 7 Gbar with a neutron yield approaching 5 ×1013 . Further target performance improvements to reach hydrodynamic equivalence to ignition on OMEGA require mitigation of cross-beam energy transfer (CBET), which reduces the laser coupling. A proposed technique to reduce CBET by driving the spherical target with overlapping laser beams having individual focal spots smaller than the outside diameter of the target was investigated. The diameter of the target was discretely varied from 800 to 1000 μm, while the laser focal spot size was kept constant at 820 μm. The larger targets driven with up to 30 kJ of laser energy used dynamic bandwidth reduction, where the smoothing by spectral dispersion (SSD) is only applied to the pickets. The smaller targets driven with 26 kJ of laser energy had SSD on the entire pulse. This talk will summarize the results of this CBET mitigation campaign and describe a path forward to achieve ignition hydro-equivalence on OMEGA. This material is based upon work supported by the Department of Energy National Nuclear Security Administration under

  2. Mutual Occultation Observations of Volcanic Hot Spots on Io in 2015

    Science.gov (United States)

    Howell, Robert R.; Spencer, John R.; Rathbun, Julie A.; Goguen, Jay D.

    2015-11-01

    During spring 2015 we observed a series of mutual occultations and eclipses of Io using the SpeX instrument on the NASA Infrared Telescope Facility (IRTF). Mutual event lightcurves were obtained on 4 Feb., 18 Feb., 15 Mar., and 22 Mar. in the Lp (3.8 micron) filter, with observations on 11 Feb. and on 8 and 10 Mar. lost to weather. Such lightcurves can potentially provide the highest spatial resolution observations of Io's volcanic hotspots possible from ground-based observations. The geometry of the recent events was particularly favorable for studies of the Loki Patera region, but unfortunately Loki remained relatively faint during the 2015 series. An eruption of Pillan was observed on 18 Feb., and is discussed in more detail by de Pater et al. (2015) at this meeting. While the relative faintness of Loki during the recent series limits the value of the new observations for studying that source, recent improvements to the Galilean satellite ephemerides also allow us to significantly improve the analysis of previous Loki occultations from 1985, 1991, 1997, and 2003. In that earlier work it was necessary to allow adjustable offsets in the relative position of the satellites to accommodate ephemeris uncertainty. That in turn limited the accuracy with which hot regions within Loki Patera could be fixed, and limited the ability to test different models for Patera activity. Initial analysis of the most recent event series indicates that such arbitrary adjustments are no longer necessary. We will present the occultation lightcurves for the most recent series and the constraints on the location of Loki activity provided by those observations and from earlier occultation series.

  3. Genetic Analysis and Gene Mapping of Light Brown Spotted Leaf Mutant in Rice

    Institute of Scientific and Technical Information of China (English)

    FENG Bao-hua; YANG Yang; SHI Yong-feng; LIN Lu; CHEN Jie; WEI Yan-lin; Hei LEUNG

    2013-01-01

    A light brown spotted-leaf mutant of rice was isolated from an ethane methyl sulfonate (EMS)induced IR64 mutant bank.The mutant,designated as Ibsl1 (light brown spotted-leaf 1),displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions.Agronomic traits including plant height,growth duration,number of filled grains per panicle,seed-setting rate and 1000-grain weight of the mutant were significantly affected.Genetic analysis showed that the mutation was controlled by a single recessive gene,tentatively named Ibsl1(t),which was mapped to the short arm of chromosome 6.By developing simple sequence repeat (SSR) markers,the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588.The Ibsl1(t) gene is likely a novel rice spotted-leaf gene since no other similar genes have been identified near the chromosomal region.The genetic data and recombination populations provided will facilitate further fine-mapping and cloning of the gene.

  4. Hot Spots and Hot Moments of Methylmercury Production Associated With Agricultural and Non-agricultural Wetlands of the Yolo Bypass Wildlife Area, California

    Science.gov (United States)

    Marvin-Dipasquale, M.; Windham-Myers, L.; Agee, J. L.; Kakouros, E.; Cox, M. H.; Fleck, J.; Alpers, C. N.; Stephenson, M.

    2008-12-01

    The Yolo Bypass Wildlife Area (YBWA) is part of the larger Yolo Bypass floodwater protection zone associated with the Sacramento River and the Sacramento-San Joaquin Delta, in California. While mercury contamination is widespread throughout the region due to historic mining practices, the Yolo Bypass is responsible for a high proportion of the aqueous methylmercury (MeHg) entering the Delta, and biota from the Yolo Bypass are particularly elevated in toxic MeHg. Land use in the YBWA includes seasonally flooded agricultural fields (white rice, wild rice, fallow fields), and permanently and seasonally flooded non-agricultural wetlands used for resident and migratory waterfowl. Mercury biogeochemistry was examined in 0-2 cm surface sediment, as a function of habitat type, wetland management, and agricultural practices during the 2007-08 crop year. In permanently flooded wetlands, MeHg concentrations varied within a narrow range (ca. 0.5-1.5 ng/g dry wt) throughout the study period. In contrast, the three types of agricultural fields had higher MeHg concentrations throughout the rice-growing season (June-Sept; ca. 1.5-3.5 ng/g), and exhibited the highest levels (ca. 3.3-6.3 ng/g) in the post-harvest winter period (Dec-Feb). Further, naturally dried sediment, sampled during July '08 from post-harvest drained fallow agricultural fields (prior to reflooding) had MeHg concentrations that were also quite elevated (3.1 +/- 1.5 ng/g). This suggests that the initial elevated concentrations of overlying water MeHg, sometimes measured soon after flooding previously dried fields, may be related to the release of MeHg formed during the previous wet season and trapped in dried sediment, as opposed to being MeHg newly produced by bacteria upon soil rewetting. These results indicate that the 'hot spots and hot moments' associated with MeHg production in this system are linked to hydrologic manipulations (wetting and drying) in the agricultural fields, and that the practice of post

  5. Identifying and Mitigating Potential Nutrient and Sediment Hot Spots under a Future Scenario in the Missouri River Basin

    Energy Technology Data Exchange (ETDEWEB)

    Wu, May [Argonne National Lab. (ANL), Argonne, IL (United States); Zhang, Zhonglong [Argonne National Lab. (ANL), Argonne, IL (United States)

    2015-09-01

    clearly attributable to the conversion of a large amount of land to switchgrass. The Middle Lower Missouri River and Lower Missouri River were identified as hot regions. Further analysis identified four subbasins (10240002, 10230007, 10290402, and 10300200) as being the most vulnerable in terms of sediment, nitrogen, and phosphorus loadings. Overall, results suggest that increasing the amount of switchgrass acreage in the hot spots should be considered to mitigate the nutrient loads. The study provides an analytical method to support stakeholders in making informed decisions that balance biofuel production and water sustainability.

  6. CTCF cis-regulates trinucleotide repeat instability in an epigenetic manner: a novel basis for mutational hot spot determination.

    Directory of Open Access Journals (Sweden)

    Randell T Libby

    2008-11-01

    Full Text Available At least 25 inherited disorders in humans result from microsatellite repeat expansion. Dramatic variation in repeat instability occurs at different disease loci and between different tissues; however, cis-elements and trans-factors regulating the instability process remain undefined. Genomic fragments from the human spinocerebellar ataxia type 7 (SCA7 locus, containing a highly unstable CAG tract, were previously introduced into mice to localize cis-acting "instability elements," and revealed that genomic context is required for repeat instability. The critical instability-inducing region contained binding sites for CTCF -- a regulatory factor implicated in genomic imprinting, chromatin remodeling, and DNA conformation change. To evaluate the role of CTCF in repeat instability, we derived transgenic mice carrying SCA7 genomic fragments with CTCF binding-site mutations. We found that CTCF binding-site mutation promotes triplet repeat instability both in the germ line and in somatic tissues, and that CpG methylation of CTCF binding sites can further destabilize triplet repeat expansions. As CTCF binding sites are associated with a number of highly unstable repeat loci, our findings suggest a novel basis for demarcation and regulation of mutational hot spots and implicate CTCF in the modulation of genetic repeat instability.

  7. Magma production rate along the Ninetyeast Ridge and its relationship to Indian plate motion and Kerguelen hot spot activity

    Science.gov (United States)

    Sreejith, K. M.; Krishna, K. S.

    2015-02-01

    The Ninetyeast Ridge, a linear trace of the Kerguelen hot spot in the Indian Ocean, was emplaced on a rapidly drifting Indian plate. Magma production rates along the ridge track are computed using gravity-derived excess crustal thickness data. The production rates change between 2 and 15 m3/s over timescales of 3-16 Myr. Major variations in magma production rates are primarily associated with significant changes in the Indian plate velocity with low-production phases linked to high plate velocity periods. The lowest magma production rate (2 m3/s) at 62 Ma is associated with the rapid northward drift of Indian plate under the influence of the Reunion mantle plume. The contemporaneous slowing of the African plate coincides with increase in magma production rate along the Walvis Ridge in the Atlantic Ocean. The present study suggests that variations in the Indian plate motion and frequent ridge jumps have a major role in controlling the magma production, particularly on long-period cycles (~16 Myr). Short-period variations (~5 Myr) in magma productions may be associated with intrinsic changes in the plume, possibly due to the presence of solitary waves in the plume conduit.

  8. Online platform for applying space-time scan statistics for prospectively detecting emerging hot spots of dengue fever.

    Science.gov (United States)

    Chen, Chien-Chou; Teng, Yung-Chu; Lin, Bo-Cheng; Fan, I-Chun; Chan, Ta-Chien

    2016-11-25

    Cases of dengue fever have increased in areas of Southeast Asia in recent years. Taiwan hit a record-high 42,856 cases in 2015, with the majority in southern Tainan and Kaohsiung Cities. Leveraging spatial statistics and geo-visualization techniques, we aim to design an online analytical tool for local public health workers to prospectively identify ongoing hot spots of dengue fever weekly at the village level. A total of 57,516 confirmed cases of dengue fever in 2014 and 2015 were obtained from the Taiwan Centers for Disease Control (TCDC). Incorporating demographic information as covariates with cumulative cases (365 days) in a discrete Poisson model, we iteratively applied space-time scan statistics by SaTScan software to detect the currently active cluster of dengue fever (reported as relative risk) in each village of Tainan and Kaohsiung every week. A village with a relative risk >1 and p value dengue fever transmission on a weekly basis at the village level by using the routine surveillance data.

  9. The HIV-1 repeated sequence R as a robust hot-spot for copy-choice recombination

    Science.gov (United States)

    Moumen, Abdeladim; Polomack, Lucette; Roques, Bernard; Buc, Henri; Negroni, Matteo

    2001-01-01

    Template switching during reverse transcription is crucial for retroviral replication. While strand transfer on the terminal repeated sequence R is essential to achieve reverse transcription, template switching from internal regions of the genome (copy choice) leads to genetic recombination. We have developed an experimental system to study copy-choice recombination in vitro along the HIV-1 genome. We identify here several genomic regions, including the R sequence, where copy choice occurred at high rates. The frequency of copy choice occurring in a given region of template was strongly influenced by the surrounding sequences, an observation that suggests a pivotal role of the folding of template RNA in the process. The sequence R, instead, constituted an exception to this rule since it was a strong hot-spot for copy choice in the different sequence contexts tested. We suggest therefore that the structure of this region has been optimised during viral evolution to ensure efficient template switching independently from the sequences that might surround it. PMID:11557813

  10. The Hot Spot Analysis: Utilization as Customized Management Tool towards Sustainable Value Chains of Companies in the Food Sector

    Directory of Open Access Journals (Sweden)

    H. Rohn

    2014-12-01

    Full Text Available The food and agricultural sector will face numerous challenges in the next decades, arising from changing global production and consumption patterns, which currently go along with high resource use, causing ecological and socio-economic impacts. The aim of this paper is to illustrate and evaluate the practical applicability of the Hot Spot Analysis methodology in the context of supply chain management in companies. The HSA is a method to identify social and ecological problems along the entire life cycle of a product. Special emphasis is put on a customized implementation in the value chain beef of McDonald’s Germany. The HSA of McDonald’s beef value chain shows that the main ecological problems arise in the phase of raw material extraction, whereas the main social problems can be identified in the phase of slaughtering. Finally, the paper shows potentials and shortcomings of such a customized application and how the results can be implemented in the sustainability management of a company.

  11. Changes in the location of biodiversity-ecosystem function hot spots across the seafloor landscape with increasing sediment nutrient loading.

    Science.gov (United States)

    Thrush, Simon F; Hewitt, Judi E; Kraan, Casper; Lohrer, A M; Pilditch, Conrad A; Douglas, Emily

    2017-04-12

    Declining biodiversity and loss of ecosystem function threatens the ability of habitats to contribute ecosystem services. However, the form of the relationship between biodiversity and ecosystem function (BEF) and how relationships change with environmental change is poorly understood. This limits our ability to predict the consequences of biodiversity loss on ecosystem function, particularly in real-world marine ecosystems that are species rich, and where multiple ecosystem functions are represented by multiple indicators. We investigated spatial variation in BEF relationships across a 300 000 m(2) intertidal sandflat by nesting experimental manipulations of sediment pore water nitrogen concentration into sites with contrasting macrobenthic community composition. Our results highlight the significance of many different elements of biodiversity associated with environmental characteristics, community structure, functional diversity, ecological traits or particular species (ecosystem engineers) to important functions of coastal marine sediments (benthic oxygen consumption, ammonium pore water concentrations and flux across the sediment-water interface). Using the BEF relationships developed from our experiment, we demonstrate patchiness across a landscape in functional performance and the potential for changes in the location of functional hot and cold spots with increasing nutrient loading that have important implications for mapping and predicating change in functionality and the concomitant delivery of ecosystem services. © 2017 The Author(s).

  12. Orographic rainfall hot spots in the Andes-Amazon transition according to the TRMM precipitation radar and in situ data

    Science.gov (United States)

    Chavez, Steven P.; Takahashi, Ken

    2017-06-01

    The Andes-Amazon transition, along the eastern Peruvian Andes, features "hot spots" with strong precipitation. Using 15 years of Tropical Rainfall Measuring Mission PR data we established a robust relation between terrain elevation and mean surface precipitation, with the latter peaking around 1000 m above sea level (asl), coinciding with the moisture flux peak of the South American Low Level Jet (SALLJ). There is strong diurnal variability, with afternoon (13-18 LT) convection in the Amazon plains, while on the eastern slopes (1000-2000 m asl), after the forcing associated with the thermal heating of the Andes subsides, convection grows during the night and surface precipitation peaks around 01-06 LT and organizes into mesoscale convective systems (MCSs). These then displace downslope to an terrain elevation of 700 m asl with stratiform regions spreading upslope and downslope and then decay during the remainder of the morning. The large MCSs contribute with at least 50% of daily rainfall (60% of the 01-06 LT rainfall). On synoptic scales, the large MCSs are more common in stronger SALLJ conditions, although subtropical cold surges are responsible for 16% of the cases.

  13. New Caledonia: A ' Hot Spot' for Valuable Chemodiversity: Part 2: Basal Angiosperms and Eudicot Rosids.

    Science.gov (United States)

    Coulerie, Paul; Poullain, Cyril

    2016-01-01

    The flora of New Caledonia encompasses more than 3000 plant species and almost 80% are endemic. New Caledonia is considered as a 'hot spot' for biodiversity. With the current global loss of biodiversity and the fact that several drugs and pesticides become obsolete, there is an urgent need to increase sampling and research on new natural products. In this context, we review the chemical knowledge available on New Caledonian native flora from economical perspectives. We expect that a better knowledge of the economic potential of plant chemistry will encourage the plantation of native plants for the development of a sustainable economy which will participate in the conservation of biodiversity. In the second part of this review, we focus on the results exposed in 60 scientific articles and describe the identification of 225 original compounds from basal angiosperms and eudicot rosids. We discuss the economic potential of plants and molecules from medicinal and industrial perspectives. This review also highlights several plants and groups, such as Amborella sp., Piperaceae, or Phyllanthaceae, that are unexplored in New Caledonia despite their high chemical interest. Those plants are considered to have priority in future chemical investigations. Copyright © 2016 Verlag Helvetica Chimica Acta AG, Zürich.

  14. Unexpected climatological behavior of MLT gravity wave momentum flux in the lee of the Southern Andes hot spot

    Science.gov (United States)

    Wit, R. J.; Janches, D.; Fritts, D. C.; Stockwell, R. G.; Coy, L.

    2017-01-01

    The Southern Argentina Agile MEteor Radar (SAAMER), located at Tierra del Fuego (53.7°S, 67.7°W), has been providing near-continuous high-resolution measurements of winds and high-frequency gravity wave (GW) momentum fluxes of the mesopause region since May 2008. As SAAMER is located in the lee of the largest seasonal GW hot spot on Earth, this is a key location to study GWs and their interaction with large-scale motions. GW momentum flux climatologies are shown for the first time for this location and discussed in light of these unique dynamics. Particularly, the large eastward GW momentum fluxes during local winter are surprising, as these observations cannot be explained by the direct upward propagation of expected large-amplitude mountain waves (MWs) through the eastward stratospheric jet. Instead, these results are interpreted as secondary GWs propagating away from stratospheric sources over the Andes accompanying MW breaking over the Southern Andes.

  15. Study and Applications of Dynamic Resistance Profiles During Resistance Spot Welding of Coated Hot-Stamping Steels

    Science.gov (United States)

    Ighodaro, Osayande Lord-Rufus; Biro, Elliot; Zhou, Y. Norman

    2017-02-01

    This work compares the role of press hardened steel coating type (Al-Si and GA) on resistance spot welding by analyzing the dynamic resistance curves measured during the weld cycles of the respective materials. It was seen that the dynamic resistance profiles for GA- and Al-Si-coated steels are similar. But the GA specimens exhibited higher resistance than Al-Si-coated specimens in the as-received condition, while the Al-Si-coated specimens exhibited higher resistance after hot stamping. From the early stages of the dynamic resistance profiles, data were obtained and applied for computing the values of components of resistances associated with the different coatings since each coating exhibits characteristic value at the early stages. The results revealed that at the start of the welding cycle, the resistance of the electrode/sheet interface was significantly higher than that of the faying surface or the bulk resistance regardless of whether the steel was Al-Si- or GA-coated. The possible uses of these resistance values in studying welding current requirement and electrode tip life were discussed.

  16. Improving the hot-spot pressure and demonstrating ignition hydrodynamic equivalence in cryogenic deuterium tritium implosions on OMEGA

    Energy Technology Data Exchange (ETDEWEB)

    Goncharov, V. N. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Sangster, T. C. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Betti, R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Boehly, T. R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Bonino, M. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Collins, T. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Craxton, R. S. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Delettrez, J. A. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Edgell, D. H. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Epstein, R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Follett, R. K. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Forrest, C. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Froula, D. H. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Yu. Glebov, V. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Harding, D. R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Henchen, R. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Hu, S. X. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Igumenshchev, I. V. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Janezic, R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Kelly, J. H. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Kessler, T. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Kosc, T. Z. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Loucks, S. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Marozas, J. A. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Marshall, F. J. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Maximov, A. V. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; McCrory, R. L. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; McKenty, P. W. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Meyerhofer, D. D. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Michel, D. T. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Myatt, J. F. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Nora, R. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Radha, P. B. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Regan, S. P. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Seka, W. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Shmayda, W. T. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Short, R.W. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Shvydky, A. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Skupsky, S. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Stoeckl, C. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Yaakobi, B. [Univ. of Rochester, NY (United States). Lab. for Laser Energetics; Frenje, J. A. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Plasma Science and Fusion Center; Gatu-Johnson, M. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Plasma Science and Fusion Center; Petrasso, R. D. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Plasma Science and Fusion Center; Casey, D. T. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-05-01

    Reaching ignition in direct-drive (DD) inertial confinement fusion implosions requires achieving central pressures in excess of 100 Gbar. The OMEGA laser system [T. R. Boehly et al., Opt. Commun. 133, 495 (1997)] is used to study the physics of implosions that are hydrodynamically equivalent to the ignition designs on the National Ignition Facility (NIF) [J. A. Paisner et al., Laser Focus World 30, 75 (1994)]. It is shown that the highest hot-spot pressures (up to 40 Gbar) are achieved in target designs with a fuel adiabat of α ≅ 4, an implosion velocity of 3.8 × 10⁷ cm/s, and a laser intensity of ~10¹⁵ W/cm². These moderate-adiabat implosions are well understood using two-dimensional hydrocode simulations. The performance of lower-adiabat implosions is significantly degraded relative to code predictions, a common feature between DD implosions on OMEGA and indirect-drive cryogenic implosions on the NIF. Simplified theoretical models are developed to gain physical understanding of the implosion dynamics that dictate the target performance. These models indicate that degradations in the shell density and integrity (caused by hydrodynamic instabilities during the target acceleration) coupled with hydrodynamics at stagnation are the main failure mechanisms in low-adiabat designs. To demonstrate ignition hydrodynamic equivalence in cryogenic implosions on OMEGA, the target-design robustness to hydrodynamic instability growth must be improved by reducing laser-coupling losses caused by cross beam energy transfer.

  17. Identification of an mtDNA mutation hot spot in UV-induced mouse skin tumors producing altered cellular biochemistry.

    Science.gov (United States)

    Jandova, Jana; Eshaghian, Alex; Shi, Mingjian; Li, Meiling; King, Lloyd E; Janda, Jaroslav; Sligh, James E

    2012-02-01

    There is increasing awareness of the role of mtDNA alterations in the development of cancer, as mtDNA point mutations are found at high frequency in a variety of human tumors. To determine the biological effects of mtDNA mutations in UV-induced skin tumors, hairless mice were irradiated to produce tumors, and the tumor mtDNAs were screened for single-nucleotide changes using temperature gradient capillary electrophoresis (TGCE), followed by direct sequencing. A mutation hot spot (9821insA) in the mitochondrially encoded tRNA arginine (mt-Tr) locus (tRNA(Arg)) was discovered in approximately one-third of premalignant and malignant skin tumors. To determine the functional relevance of this particular mutation in vitro, cybrid cell lines containing different mt-Tr (tRNA(Arg)) alleles were generated. The resulting cybrid cell lines contained the same nuclear genotype and differed only in their mtDNAs. The biochemical analysis of the cybrids revealed that the mutant haplotype is associated with diminished levels of complex I protein (CI), resulting in lower levels of baseline oxygen consumption and lower cellular adenosine triphosphate (ATP) production. We hypothesize that this specific mtDNA mutation alters cellular biochemistry, supporting the development of keratinocyte neoplasia.

  18. Unexpected Climatological Behavior of MLT Gravity Wave Momentum Flux in the Lee of the Southern Andes Hot Spot

    Science.gov (United States)

    DeWit, R. J.; Janches, D.; Fritts, D. C.; Stockwell, R. G.; Coy, L.

    2017-01-01

    The Southern Argentina Agile MEteor Radar (SAAMER), located at Tierra del Fuego (53.7degS, 67.7degW), has been providing near-continuous high-resolution measurements of winds and high-frequency gravity wave (GW) momentum fluxes of the mesopause region since May 2008. As SAAMER is located in the lee of the largest seasonal GW hot spot on Earth, this is a key location to study GWs and their interaction with large-scale motions. GW momentum flux climatologies are shown for the first time for this location and discussed in light of these unique dynamics. Particularly, the large eastward GW momentum fluxes during local winter are surprising, as these observations cannot be explained by the direct upward propagation of expected large-amplitude mountain waves (MWs) through the eastward stratospheric jet. Instead, these results are interpreted as secondary GWs propagating away from stratospheric sources over the Andes accompanying MW breaking over the Southern Andes.

  19. Current dependence of the hot-spot response spectrum of superconducting single-photon detectors with different layouts

    Science.gov (United States)

    Charaev, I.; Semenov, A.; Doerner, S.; Gomard, G.; Ilin, K.; Siegel, M.

    2017-02-01

    We show that avoiding bends in a current-carrying superconducting nanowire enhances the probability for low energy photons to be detected and that this enhancement is entirely due to the increase in the experimentally achievable critical current. We studied nanowires shaped as either meander or spiral. The spirals had different layouts, a double-spiral layout with an S-turn in the middle and a single-spiral layout without such a turn. Nanowires were prepared from films of niobium nitride with a thickness of 5 nm. For specimens with each layout we measured the spectra of the single-photon response in the wavelength range from 400 nm to 1600 nm and defined the cut-off wavelength (λ c) beyond which the response rolls off. The largest and the smallest λ c were found for the single-spiral layout and for the meander, respectively. For all three layouts the relationship between λ c and the relative bias current falls onto a universal curve which has been predicted earlier in the framework of the modified hot-spot model. For the single-spiral layout, the efficiency of photon detection at wavelengths smaller than λ c reaches the expected absorbance of the spiral structure and the timing jitter per unit length of the nanowire has the smallest value.

  20. Satellite thermal monitoring of the 2010 - 2013 eruption of Kizimen volcano (Kamchatka) using MIROVA hot-spot detection system

    Science.gov (United States)

    Massimetti, Francesco; Coppola, Diego; Laiolo, Marco; Cigolini, Corrado

    2017-04-01

    After 81 years of rest, the Holocenic stratovolcano of Kizimen (Kamchatka, Russia) began a new eruptive phase on December 2010. The eruption was preceded by a year-long seismic unrest and fumarole activity, and persisted for 3 years showing a transition from explosive to effusive style. The initial explosive phase caused the partial disruption of the volcano summit and was followed by the effusion of andesitic lava flow along the eastern side of the edifice. Here we used an automatic hot-spot detection system named MIROVA (Middle InfraRed Observation of Volcanic Activity), in order to track the thermal evolution of the eruption and to understand the eruptive dynamic. MIROVA is based on the analysis IR images acquired by the MODIS sensor (Moderate Resolution Imaging Spectroradiometer) and is able to provide thermal maps (1 km resolution) and Volcanic Radiative Power (VRP, in Watt) time series in near real time (1-4 hours from satellite overpass). Each image with a thermal alert has been classified, distinguishing different quality level of the data based on cloud cover, viewing geometry and coherence with the VRP trend. The analysis of VRP variation show different thermal phases that have been correlated with independent observations of KVERT (Kamchatka Volcanic Eruption Response Team). Finally, we show that the relation between total thermal energy radiated (VRE, in Joule) and erupted lava volume is consistent with the typical radiant density of an intermediate-silicic lava flow (Coppola et al., 2013).

  1. Germline Stem Cell Competition, Mutation Hot Spots, Genetic Disorders, and Older Fathers.

    Science.gov (United States)

    Arnheim, Norman; Calabrese, Peter

    2016-08-31

    Some de novo human mutations arise at frequencies far exceeding the genome average mutation rate. Examples include the common mutations at one or a few sites in the genes that cause achondroplasia, Apert syndrome, multiple endocrine neoplasia type 2B, and Noonan syndrome. These mutations are recurrent, provide a gain of function, are paternally derived, and are more likely to be transmitted as the father ages. Recent experiments have tested whether the high mutation frequencies are due to an elevated mutation rate per cell division, as expected, or to an advantage of the mutant spermatogonial stem cells over wild-type stem cells. The evidence, which includes the surprising discovery of testis mutation clusters, rules out the former model but not the latter. We propose how the mutations might alter spermatogonial stem cell function and discuss how germline selection contributes to the paternal age effect, the human mutational load, and adaptive evolution.

  2. Three-dimensional (3D) plasmonic hot spots for label-free sensing and effective photothermal killing of multiple drug resistant superbugs.

    Science.gov (United States)

    Jones, Stacy; Sinha, Sudarson Sekhar; Pramanik, Avijit; Ray, Paresh Chandra

    2016-11-03

    Drug resistant superbug infection is one of the foremost threats to human health. Plasmonic nanoparticles can be used for ultrasensitive bio-imaging and photothermal killing by amplification of electromagnetic fields at nanoscale "hot spots". One of the main challenges to plasmonic imaging and photothermal killing is design of a plasmonic substrate with a large number of "hot spots". Driven by this need, this article reports design of a three-dimensional (3D) plasmonic "hot spot"-based substrate using gold nanoparticle attached hybrid graphene oxide (GO), free from the traditional 2D limitations. Experimental results show that the 3D substrate has capability for highly sensitive label-free sensing and generates high photothermal heat. Reported data using p-aminothiophenol conjugated 3D substrate show that the surface enhanced Raman spectroscopy (SERS) enhancement factor for the 3D "hot spot"-based substrate is more than two orders of magnitude greater than that for the two-dimensional (2D) substrate and five orders of magnitude greater than that for the zero-dimensional (0D) p-aminothiophenol conjugated gold nanoparticle. 3D-Finite-Difference Time-Domain (3D-FDTD) simulation calculations indicate that the SERS enhancement factor can be greater than 10(4) because of the bent assembly structure in the 3D substrate. Results demonstrate that the 3D-substrate-based SERS can be used for fingerprint identification of several multi-drug resistant superbugs with detection limits of 5 colony forming units per mL. Experimental data show that 785 nm near infrared (NIR) light generates around two times more photothermal heat for the 3D substrate with respect to the 2D substrate, and allows rapid and effective killing of 100% of the multi-drug resistant superbugs within 5 minutes.

  3. Strategy to eliminate catalyst hot-spots in the partial oxidation of methane: enhancing its activity for direct hydrogen production by reducing the reactivity of lattice oxygen.

    Science.gov (United States)

    Wen, Cun; Liu, Yi; Guo, Yun; Wang, Yanqin; Lu, Guanzhong

    2010-02-14

    Hydrogen can be produced over Er(2)O(3) in methane oxidation (oxygen/methane = 26). The reactivity of lattice oxygen in the catalyst plays a main role in the conversion of surface hydroxyl species to hydrogen or water. Adding a rare earth element into a catalyst can reduce the reactivity of lattice oxygen, resulting in increased hydrogen production, to eliminate catalyst hot-spots.

  4. Bone Scan "Hot Spot" at the Superior Lateral Orbital Margin Fronto-zygomatic Suture Uptake Characterized with Tc-99m MDP SPECT/CT.

    Science.gov (United States)

    Thang, S P; Tan, A E H; Goh, A S W

    2011-07-01

    Findings of a solitary "hot spot" at the superior lateral orbital margin on bone scan scintigraphy is not uncommonly seen, and is often dismissed as a benign lesion. However, the exact etiology is indeterminate. We present two cases in which hybrid single photon emission computed tomography/computed tomography imaging was able to characterize and localize this uptake, demonstrating correlation to the right fronto-zygomatic suture.

  5. Do we miss the hot spots? – The use of very high resolution aerial photographs to quantify carbon fluxes in peatlands

    Directory of Open Access Journals (Sweden)

    T. Becker

    2008-10-01

    Full Text Available Accurate determination of carbon balances in heterogeneous ecosystems often requires the extrapolation of point based measurements. The ground resolution (pixel size of the extrapolation base, e.g. a land-cover map, might thus influence the calculated carbon balance, in particular if biogeochemical hot spots are small in size. In this paper, we test the effects of varying ground resolution on the calculated carbon balance of a boreal peatland consisting of hummocks (dry, lawns (intermediate and flarks (wet surfaces. The generalizations in lower resolution imagery led to biased area estimates for individual micro-site types. While areas of lawns and hummocks were stable below a threshold resolution of ~60 cm, the maximum of the flark area was located at resolutions below 25 cm and was then decreasing with coarsening resolution. Using a resolution of 100 cm instead of 6 cm led to an overestimation of total CO2 uptake of the studied peatland area (approximately 14 600 m2 of ~5% and an underestimation of total CH4 emission of ~6%. To accurately determine the surface area of scattered and small-sized micro-site types in heterogeneous ecosystems (e.g. flarks in peatlands, a minimum ground resolution appears necessary. In our case this leads to a recommended resolution of 25 cm, which can be derived by conventional airborne imagery. The usage of high resolution imagery from commercial satellites, e.g. Quickbird, however, is likely to underestimate the surface area of biogeochemical hot spots. It is important to note that the observed resolution effect on the carbon balance estimates can be much stronger for other ecosystems than for the investigated peatland. In the investigated peatland the relative hot spot area of the flarks is very small and their hot spot characteristics with respect to CH4 and CO2 fluxes is rather modest.

  6. Demonstration of Fuel Hot-Spot Pressure in Excess of 50 Gbar for Direct-Drive, Layered Deuterium-Tritium Implosions on OMEGA.

    Science.gov (United States)

    Regan, S P; Goncharov, V N; Igumenshchev, I V; Sangster, T C; Betti, R; Bose, A; Boehly, T R; Bonino, M J; Campbell, E M; Cao, D; Collins, T J B; Craxton, R S; Davis, A K; Delettrez, J A; Edgell, D H; Epstein, R; Forrest, C J; Frenje, J A; Froula, D H; Gatu Johnson, M; Glebov, V Yu; Harding, D R; Hohenberger, M; Hu, S X; Jacobs-Perkins, D; Janezic, R; Karasik, M; Keck, R L; Kelly, J H; Kessler, T J; Knauer, J P; Kosc, T Z; Loucks, S J; Marozas, J A; Marshall, F J; McCrory, R L; McKenty, P W; Meyerhofer, D D; Michel, D T; Myatt, J F; Obenschain, S P; Petrasso, R D; Radha, P B; Rice, B; Rosenberg, M J; Schmitt, A J; Schmitt, M J; Seka, W; Shmayda, W T; Shoup, M J; Shvydky, A; Skupsky, S; Solodov, A A; Stoeckl, C; Theobald, W; Ulreich, J; Wittman, M D; Woo, K M; Yaakobi, B; Zuegel, J D

    2016-07-08

    A record fuel hot-spot pressure P_{hs}=56±7  Gbar was inferred from x-ray and nuclear diagnostics for direct-drive inertial confinement fusion cryogenic, layered deuterium-tritium implosions on the 60-beam, 30-kJ, 351-nm OMEGA Laser System. When hydrodynamically scaled to the energy of the National Ignition Facility, these implosions achieved a Lawson parameter ∼60% of the value required for ignition [A. Bose et al., Phys. Rev. E 93, 011201(R) (2016)], similar to indirect-drive implosions [R. Betti et al., Phys. Rev. Lett. 114, 255003 (2015)], and nearly half of the direct-drive ignition-threshold pressure. Relative to symmetric, one-dimensional simulations, the inferred hot-spot pressure is approximately 40% lower. Three-dimensional simulations suggest that low-mode distortion of the hot spot seeded by laser-drive nonuniformity and target-positioning error reduces target performance.

  7. Hot Spot and THz Wave Generation in Bi2Sr2CaCu2O8 Intrinsic Josephson Junction Stacks

    Science.gov (United States)

    Kleiner, Reinhold

    2013-03-01

    Stacks of intrinsic Josephson junctions made of the high temperature superconductor Bi2Sr2CaCu2O8 have been shown to emit coherent radiation at THz frequencies. Emission is observed both in a low bias regime and a high bias regime. While at low bias the temperature of the stack is close to the bath temperature, at high bias a hot spot and a standing wave, formed in the ``cold'' part of the stack, coexist. THz radiation is very stable in this regime, exhibiting a linewidth which is much smaller than expected from a purely cavity-induced synchronization mechanism. We investigate the interaction of hot spots and THz waves using a combination of transport measurement, direct electromagnetic wave detection and low temperature scanning laser microscopy (LTSLM). In this talk recent developments will be presented, with a focus on the mechanism of hot spot formation. In collaboration with B. Gross, S. Guénon, M. Y. Li, J. Yuan, N. Kinev, J. Li, A. Ishii, K. Hirata, T. Hatano, R. G. Mints, D. Koelle, V. P. Koshelets, H. B. Wang and P. H. Wu.

  8. Mobile Launch Platform Vehicle Assembly Building Area (SWMU 056) Hot Spot 3 Bioremediation Interim Measures Work Plan, Kennedy Space Center, Florida

    Science.gov (United States)

    Whitney L. Morrison; Daprato, Rebecca C.

    2016-01-01

    This Interim Measures Work Plan (IMWP) presents an approach and design for the remediation of chlorinated volatile organic compound (CVOC) groundwater impacts using bioremediation (biostimulation and bioaugmentation) in Hot Spot 3, which is defined by the area where CVOC (trichloroethene [TCE], cis-1,2-dichloroethene [cDCE], and vinyl chloride [VC]) concentrations are greater than 10 times their respective Florida Department of Environmental Protection (FDEP) Natural Attenuation Default Concentration (NADC) [10xNADC] near the western Mobile Launch Platform (MLP) structure. The IM treatment area is the Hot Spot 3 area, which is approximately 0.07 acres and extends from approximately 6 to 22 and 41 to 55 feet below land surface (ft BLS). Within Hot Spot 3, a source zone (SZ; area with TCE concentrations greater than 1% solubility [11,000 micrograms per liter (micrograms/L)]) was delineated and is approximately 0.02 acres and extends from approximately 6 to 16 and 41 to 50 ft BLS.

  9. Fine genetic mapping of target leaf spot resistance gene cca-3 in cucumber, Cucumis sativus L

    Science.gov (United States)

    The target leaf spot (TLS) is a very important fungal disease in cucumber. In this study, we conducted fine genetic mapping of a recessively inherited resistance gene, cca-2 against TLS with 1,083 F2 plants derived from the resistant cucumber inbred line D31 and the susceptible line D5. Initial mapp...

  10. The color of a Dalmatian's spots: Linkage evidence to support the TYRP1 gene

    Directory of Open Access Journals (Sweden)

    Strain George M

    2005-07-01

    Full Text Available Abstract Background The distinctive coat pattern of a Dalmatian is the result of the interaction of several loci. While the encoded function of these genes is not fully understood, it is known the Piebald, Ticking, and Flecking loci interact to produce the Dalmatian's classic pigmented spots on a white background. The color of the pigmented spots in purebred Dalmatians can either be black or liver, but the locus responsible for color determination is unknown. Studies have been conducted to determine the underlying genes involved in coat color determination in the dog, e.g., in the Labrador Retriever, but none to date have addressed black versus liver in the Dalmatian. Results A genome scan was conducted in a multi-generational kindred of Dalmatians segregating black and liver spot color. Linkage analysis was performed using a total of 113 polymorphic microsatellite markers from the kindred. Linkage was found between spot color and a single microsatellite marker, FH2319 (LOD = 12.5 on chromosome 11. Conclusion The TYRP1 (Brown locus is located at position 50.1 Mb on chromosome 11, which is approximately 0.4 Mb from marker FH2319. Given the recent characterization of TYRP1 genetic variations in the dog and the linkage evidence reported here, TYRP1 is likely responsible for the spot color variation of black versus liver seen in the Dalmatian.

  11. Dioxins and Nonortho PCBs in Breast Milk of Vietnamese Mothers Living in the Largest Hot Spot of Dioxin Contamination.

    Science.gov (United States)

    Nghi, Tran Ngoc; Nishijo, Muneko; Manh, Ho Dung; Tai, Pham The; Van Luong, Hoang; Anh, Tran Hai; Thao, Pham Ngoc; Trung, Nguyen Viet; Waseda, Tomoo; Nakagawa, Hideaki; Kido, Teruhiko; Nishijo, Hisao

    2015-05-05

    Bien Hoa Air Base is the largest dioxin contamination hot spot in Vietnam. In 2012, we recruited 216 mothers who were living in 10 communities around Bien Hoa Air Base and had delivered newborns at a prefecture hospital, and we investigated recent exposure levels of dioxins and nonortho PCBs in their breast milk. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-tetraCDD) was present at 2.6 pg/g lipid in primiparae and 2.2 pg/g lipid in multiparae. Among multiparae and total subjects, significant high prevalence of 2,3,7,8-tetraCDD≥5 pg/g lipid and 2,3,7,8-tetraCDD contribution≥40% were observed in mothers living in the five communities closest to Bien Hoa Air Base. The TEQ for nonortho PCBs was 1.6 pg-TEQ/g lipid for primiparae, and this was even lower than that in the unsprayed area. The length of residency was a strong factor to increase dioxins, including 2,3,7,8-tetraCDD. Residency in the five communities with the highest exposure was a specific risk factor for increased 2,3,7,8-tetraCDD in breast milk. Food intake might contribute partly to the increased levels of dioxin congeners other than 2,3,7,8-tetraCDD in breast milk. These results suggest that Bien Hoa Air Base has led to elevated 2,3,7,8-tetraCDD levels in breast milk of mothers in nearby areas even in the recent years.

  12. Multi-Scale Multi-physics Methods Development for the Calculation of Hot-Spots in the NGNP

    Energy Technology Data Exchange (ETDEWEB)

    Downar, Thomas [Univ. of Michigan, Ann Arbor, MI (United States); Seker, Volkan [Univ. of Michigan, Ann Arbor, MI (United States)

    2013-04-30

    Radioactive gaseous fission products are released out of the fuel element at a significantly higher rate when the fuel temperature exceeds 1600°C in high-temperature gas-cooled reactors (HTGRs). Therefore, it is of paramount importance to accurately predict the peak fuel temperature during all operational and design-basis accident conditions. The current methods used to predict the peak fuel temperature in HTGRs, such as the Next-Generation Nuclear Plant (NGNP), estimate the average fuel temperature in a computational mesh modeling hundreds of fuel pebbles or a fuel assembly in a pebble-bed reactor (PBR) or prismatic block type reactor (PMR), respectively. Experiments conducted in operating HTGRs indicate considerable uncertainty in the current methods and correlations used to predict actual temperatures. The objective of this project is to improve the accuracy in the prediction of local "hot" spots by developing multi-scale, multi-physics methods and implementing them within the framework of established codes used for NGNP analysis.The multi-scale approach which this project will implement begins with defining suitable scales for a physical and mathematical model and then deriving and applying the appropriate boundary conditions between scales. The macro scale is the greatest length that describes the entire reactor, whereas the meso scale models only a fuel block in a prismatic reactor and ten to hundreds of pebbles in a pebble bed reactor. The smallest scale is the micro scale--the level of a fuel kernel of the pebble in a PBR and fuel compact in a PMR--which needs to be resolved in order to calculate the peak temperature in a fuel kernel.

  13. Sustainability of Public Health Interventions to Reduce the Risk of Dioxin Exposure at Severe Dioxin Hot Spots in Vietnam.

    Science.gov (United States)

    Tuyet-Hanh, Tran Thi; Vu-Anh, Le; Dunne, Michael; Toms, Leisa-Maree; Tenkate, Thomas; Harden, Fiona

    2015-08-01

    Severe dioxin contamination at Bien Hoa and Da Nang airbases, Vietnam is of international concern. Public Health risk reduction programs were implemented in Bien Hoa in 2007-2009 and in Da Nang in 2009-2011. In 2009 and 2011 we reported the encouraging results of these interventions in improving the knowledge, attitude and practices (KAP) of local residents in reducing the dioxin exposure risk through foods. In 2013 we revisited these dioxin hot spots, aimed to evaluate whether the results of the intervention were maintained and to identify factors affecting the sustainability of the programs. To assess this, 16 in-depth interviews, six focus group discussions, and pre and post intervention KAP surveys were undertaken. 800 respondents from six intervention wards and 200 respondents from Buu Long Ward (the control site) were randomly selected to participate in the surveys. The results showed that as of 2013, the programs were rated as "moderately sustained" with a score of 3.3 out of 5.0 (cut off points 2.5 to <3.5) for Bien Hoa, and "well sustained" with a score of 3.8 out of 5.0 (cut off points 3.5 to <4.5) for Da Nang. Most formal intervention program activities had ceased and dioxin risk communication activities were no longer integrated into local routine health education programs. However, the main outcomes were maintained and were better than that in the control ward. Migration, lack of official guidance from City People's Committees and local authorities as well as the politically sensitive nature of dioxin issues were the main challenges for the sustainability of the programs.

  14. Opening the research agenda for selection of hot spots for human biomonitoring research in Belgium: a participatory research project

    Directory of Open Access Journals (Sweden)

    Chovanova Hana

    2010-07-01

    Full Text Available Abstract Background In order to select priority hotspots for environment and health research in Flanders (Belgium, an open procedure was organized. Environment and health hotspots are strong polluting point sources with possible health effects for residents living in the vicinity of the hot spot. The selection procedure was part of the work of the Flemish Centre of Expertise for Environment and Health, which investigates the relation between environmental pollution and human health. The project is funded and steered by the Flemish government. Methods The involvement of other actors than merely experts is inspired by the 'analytical-deliberative' approach of the National Research Council in the United States and the extended peer community approach. These approaches stress the importance of involving different expert- and social perspectives in order to increase the knowledge base on complex issues. In the procedure used in the project a combination of expert and stakeholder input was essential. The final decision was supported by a multi-criteria analysis of expert assessment and stakeholder advice. Results The endeavour was challenging from the start because of the complicated ambition of including a diversity of actors, potential hotspots, concerns and assessment criteria, but nevertheless the procedure proved its value in both structuring and informing the decision-making process. Moreover the process gained the support of most actors participating in the process, even though the final selection could not satisfy all preferences. Conclusions Opening the research agenda exemplifies the value of inter- and transdisciplinary cooperation as well as the need for a well-structured and negotiated procedure that combines relevant factors and actors with pragmatism. The value of such a process also needs to prove itself in practice after the procedure has been completed: the tension between an ambition of openness on the one hand and a more closed

  15. Premutation huntingtin allele adopts a non-B conformation and contains a hot spot for DNA damage

    Energy Technology Data Exchange (ETDEWEB)

    Jarem, Daniel A. [Department of Chemistry, Brown University, Providence, RI 02912 (United States); Delaney, Sarah, E-mail: sarah_delaney@brown.edu [Department of Chemistry, Brown University, Providence, RI 02912 (United States)

    2011-12-09

    Highlights: Black-Right-Pointing-Pointer First structural and thermodynamic analysis of premutation allele of HD. Black-Right-Pointing-Pointer Premutation allele of HD adopts a stem-loop non-B conformation. Black-Right-Pointing-Pointer Healthy and premutation length stem-loops are hyper-susceptible to oxidative damage. Black-Right-Pointing-Pointer Stability of stem-loop structures increases linearly with repeat length. Black-Right-Pointing-Pointer Thermodynamic stability, not the ability to adopt non-B conformation, distinguishes DNA prone to expansion from stable DNA. -- Abstract: The expansion of a CAG trinucleotide repeat (TNR) sequence has been linked to several neurological disorders, for example, Huntington's disease (HD). In HD, healthy individuals have 5-35 CAG repeats. Those with 36-39 repeats have the premutation allele, which is known to be prone to expansion. In the disease state, greater than 40 repeats are present. Interestingly, the formation of non-B DNA conformations by the TNR sequence is proposed to contribute to the expansion. Here we provide the first structural and thermodynamic analysis of a premutation length TNR sequence. Using chemical probes of nucleobase accessibility, we found that similar to (CAG){sub 10}, the premutation length sequence (CAG){sub 36} forms a stem-loop hairpin and contains a hot spot for DNA damage. Additionally, calorimetric analysis of a series of (CAG){sub n} sequences, that includes repeat tracts in both the healthy and premutation ranges, reveal that thermodynamic stability increases linearly with the number of repeats. Based on these data, we propose that while non-B conformations can be formed by TNR tracts found in both the healthy and premutation allele, only sequences containing at least 36 repeats have sufficient thermodynamic stability to contribute to expansion.

  16. Improving the hot-spot pressure and demonstrating ignition hydrodynamic equivalence in cryogenic deuterium–tritium implosions on OMEGA

    Energy Technology Data Exchange (ETDEWEB)

    Goncharov, V. N.; Sangster, T. C.; Betti, R.; Boehly, T. R.; Bonino, M. J.; Collins, T. J. B.; Craxton, R. S.; Delettrez, J. A.; Edgell, D. H.; Epstein, R.; Follett, R. K.; Forrest, C. J.; Froula, D. H.; Glebov, V. Yu.; Harding, D. R.; Henchen, R. J.; Hu, S. X.; Igumenshchev, I. V.; Janezic, R.; Kelly, J. H. [Laboratory for Laser Energetics, University of Rochester, Rochester, New York 14623 (United States); and others

    2014-05-15

    Reaching ignition in direct-drive (DD) inertial confinement fusion implosions requires achieving central pressures in excess of 100 Gbar. The OMEGA laser system [T. R. Boehly et al., Opt. Commun. 133, 495 (1997)] is used to study the physics of implosions that are hydrodynamically equivalent to the ignition designs on the National Ignition Facility (NIF) [J. A. Paisner et al., Laser Focus World 30, 75 (1994)]. It is shown that the highest hot-spot pressures (up to 40 Gbar) are achieved in target designs with a fuel adiabat of α ≃ 4, an implosion velocity of 3.8 × 10{sup 7} cm/s, and a laser intensity of ∼10{sup 15} W/cm{sup 2}. These moderate-adiabat implosions are well understood using two-dimensional hydrocode simulations. The performance of lower-adiabat implosions is significantly degraded relative to code predictions, a common feature between DD implosions on OMEGA and indirect-drive cryogenic implosions on the NIF. Simplified theoretical models are developed to gain physical understanding of the implosion dynamics that dictate the target performance. These models indicate that degradations in the shell density and integrity (caused by hydrodynamic instabilities during the target acceleration) coupled with hydrodynamics at stagnation are the main failure mechanisms in low-adiabat designs. To demonstrate ignition hydrodynamic equivalence in cryogenic implosions on OMEGA, the target-design robustness to hydrodynamic instability growth must be improved by reducing laser-coupling losses caused by cross beam energy transfer.

  17. Quantifying the evidence of climate change in the light of uncertainty exemplified by the Mediterranean hot spot region

    Science.gov (United States)

    Paeth, Heiko; Vogt, Gernot; Paxian, Andreas; Hertig, Elke; Seubert, Stefanie; Jacobeit, Jucundus

    2017-04-01

    Climate change projections are subject to uncertainty arising from climate model deficiencies, unknown initial conditions and scenario assumptions. In the IPCC reports and many other publications climate changes and uncertainty ranges are usually displayed in terms of multi-model ensemble means and confidence intervals, respectively. In this study, we present a more quantitative assessment and statistical testing of climate change signals in the light of uncertainty. The approach is based on a two-way analysis of variance, referring to 24 climate models from the CMIP3 multi-model ensemble, and extents over the 21st century. The method also distinguishes between different climate variables, time scales and emission scenarios and is combined with a simple bias correction algorithm. The Mediterranean region has been chosen as a case study because it represents an assumed hot spot of future climate change, where temperature is projected to rise substantially and precipitation may decrease dramatically by the end of the 21st century. It is found that future temperature variations are mainly determined by radiative forcing, accounting for up to 60% of total variability, especially in the western Mediterranean Basin. In contrast, future precipitation variability is almost completely attributable to model uncertainty and model internal variability, both being important in more or less equal shares. This general finding is slightly depending on the prescribed emission scenario and strictly sensitive to the considered time scale. In contrast to precipitation, the temperature signal can be enhanced noticeably when bias-correcting the models' climatology during the 20th century: the greenhouse signal then accounts for up to 75% of total temperature variability in the regional mean.

  18. High prevalence of diverse forms of HIV-1 intersubtype recombinants in Central Myanmar: geographical hot spot of extensive recombination.

    Science.gov (United States)

    Takebe, Yutaka; Motomura, Kazushi; Tatsumi, Masashi; Lwin, Hla Htut; Zaw, Myint; Kusagawa, Shigeru

    2003-09-26

    To investigate the molecular epidemiology and genetic structure of HIV-1s causing the epidemic in Central Myanmar and to explore the genesis of HIV epidemic in this area. A molecular epidemiological investigation was conducted in 1999-2000 in the city of Mandalay among high-risk populations and the structural features of circulating HIV-1s were analyzed. HIV-1 genotypes of 59 specimens were screened based on gag (p17) and env (C2/V3) regions. Near full-length nucleotide sequences of HIV-1 isolates with subtype discordance were determined and their recombinant structures were characterized. Three lineages of HIV-1 strains, including CRF01_AE (27, 45.8%), subtype B' (Thailand variant of subtype B) (15, 25.4%) and subtype C (8, 13.6%), were distributed in Mandalay, while substantial portions (9, 15.3%) of specimens showed various patterns of subtype discordance in different regions of HIV-1 genomes. The study on six HIV-1 isolates with subtype discordance revealed that they were highly diverse types of unique recombinant forms (URFs) comprised of various combinations of three circulating subtypes. One URF was a particularly complex mosaic that contained 13 recombination breakpoints between three HIV-1 subtypes. Approximately half of recombinants showed 'pseudotype' virion structures, in which the external portions of envelope glycoproteins were exchanged with different lineages of HIV-1 strains, suggesting the potential selective advantage of 'pseudotype' viruses over parental strains. The study revealed the unique geographical hot spot in Central Myanmar where extensive recombination events appeared to be taking place continually. This reflects the presence of highly exposed individuals and social networks of HIV-1 transmission.

  19. Catchments as heterogeneous and multi-species reactors: An integral approach for identifying biogeochemical hot-spots at the catchment scale

    Science.gov (United States)

    Weyer, Christina; Peiffer, Stefan; Schulze, Kerstin; Borken, Werner; Lischeid, Gunnar

    2014-11-01

    From a biogeochemical perspective, catchments can be regarded as reactors that transform the input of various substances via precipitation or deposition as they pass through soils and aquifers towards draining streams. Understanding and modeling the variability of solute concentrations in catchment waters require the identification of the prevailing processes, determining their respective contribution to the observed transformation of substances, and the localization of "hot spots", that is, the most reactive areas of catchments. For this study, we applied a non-linear variant of the Principle Component Analysis, the Isometric Feature Mapping (Isomap), to a data set composed of 1686 soil solution, groundwater and stream water samples and 16 variables (Al, Ca, Cl, Fe, K, Mg, Mn, Na, NH4, NO3, SO4, total S, Si, DOC, electric conductivity and pH values) from the Lehstenbach catchment in Germany. The aim was (i) to assess the contribution of the prevailing biogeochemical processes to the variability of solute concentrations in water samples taken from soils, in groundwater and in stream water in a catchment and (ii) to identify hot spots at the catchment scale with respect to 16 solutes along different flow paths. The first three dimensions of the Isomap analysis explained 48%, 30% and 11%, respectively, i.e. 89% of the variance in the data set. Scores of the first three dimensions could be ascribed to three predominating bundles of biogeochemical processes: (i) redox processes, (ii) acid-induced podzolization, and (iii) weathering processes. In general, the upper 1 m topsoil layer could be considered as hot spots along flow paths from upslope soils and in the wetland, although with varying extents for the different prevailing biogeochemical processes. Nearly 67% and 97% of the variance with respect to redox processes and acid induced podzolization could be traced back to hot spots, respectively, representing less than 2% of the total spatial volume of the catchment

  20. Introduction and evaluation of a hot spots priming kinetics in a two-dimensional code; Introduction et evaluation d`une cinetique d`amorcage par points chauds dans un code bidimensionnel

    Energy Technology Data Exchange (ETDEWEB)

    Vong, T.S.; Leroy, M. [CEA Vaujours-Moronvilliers, 77 - Courtry (France)

    1996-12-31

    In this paper, the kinetics of hot spots priming in heterogenous explosives is introduced into a 2D hydrodynamical code. The AMORC kinetics model takes into account the two priming phases (formation, firing of hot spots and combustion of grains) and is evaluated in various experimental configurations with 1D and 2D symmetries. A TATB-based composition was used for this first evaluation. (J.S.) 7 refs.

  1. Spatial and Temporal Hot Spots of Aedes albopictus Abundance inside and outside a South European Metropolitan Area.

    Directory of Open Access Journals (Sweden)

    Mattia Manica

    2016-06-01

    Full Text Available Aedes albopictus is a tropical invasive species which in the last decades spread worldwide, also colonizing temperate regions of Europe and US, where it has become a public health concern due to its ability to transmit exotic arboviruses, as well as severe nuisance problems due to its aggressive daytime outdoor biting behaviour. While several studies have been carried out in order to predict the potential limits of the species expansions based on eco-climatic parameters, few studies have so far focused on the specific effects of these variables in shaping its micro-geographic abundance and dynamics. The present study investigated eco-climatic factors affecting Ae. albopictus abundance and dynamics in metropolitan and sub-urban/rural sites in Rome (Italy, which was colonized in 1997 and is nowadays one of the most infested metropolitan areas in Southern Europe. To this aim, longitudinal adult monitoring was carried out along a 70 km-transect across and beyond the most urbanized and densely populated metropolitan area. Two fine scale spatiotemporal datasets (one with reference to a 20m circular buffer around sticky traps used to collect mosquitoes and the second to a 300m circular buffer within each sampling site were exploited to analyze the effect of climatic and socio-environmental variables on Ae. albopictus abundance and dynamics along the transect. Results showed an association between highly anthropized habitats and high adult abundance both in metropolitan and sub-urban/rural areas, with "small green islands" corresponding to hot spots of abundance in the metropolitan areas only, and a bimodal seasonal dynamics with a second peak of abundance in autumn, due to heavy rains occurring in the preceding weeks in association with permissive temperatures. The results provide useful indications to prioritize public mosquito control measures in temperate urban areas where nuisance, human-mosquito contact and risk of local arbovirus transmission are

  2. Size-segregated aerosol in a hot-spot pollution urban area: Chemical composition and three-way source apportionment.

    Science.gov (United States)

    Bernardoni, V; Elser, M; Valli, G; Valentini, S; Bigi, A; Fermo, P; Piazzalunga, A; Vecchi, R

    2017-08-24

    In this work, a comprehensive characterisation and source apportionment of size-segregated aerosol collected using a multistage cascade impactor was performed. The samples were collected during wintertime in Milan (Italy), which is located in the Po Valley, one of the main pollution hot-spot areas in Europe. For every sampling, size-segregated mass concentration, elemental and ionic composition, and levoglucosan concentration were determined. Size-segregated data were inverted using the program MICRON to identify and quantify modal contributions of all the measured components. The detailed chemical characterisation allowed the application of a three-way (3-D) receptor model (implemented using Multilinear Engine) for size-segregated source apportionment and chemical profiles identification. It is noteworthy that - as far as we know - this is the first time that three-way source apportionment is attempted using data of aerosol collected by traditional cascade impactors. Seven factors were identified: wood burning, industry, resuspended dust, regional aerosol, construction works, traffic 1, and traffic 2. Further insights into size-segregated factor profiles suggested that the traffic 1 factor can be associated to diesel vehicles and traffic 2 to gasoline vehicles. The regional aerosol factor resulted to be the main contributor (nearly 50%) to the droplet mode (accumulation sub-mode with modal diameter in the range 0.5-1 μm), whereas the overall contribution from the two factors related to traffic was the most important one in the other size modes (34-41%). The results showed that applying a 3-D receptor model to size-segregated samples allows identifying factors of local and regional origin while receptor modelling on integrated PM fractions usually singles out factors characterised by primary (e.g. industry, traffic, soil dust) and secondary (e.g. ammonium sulphate and nitrate) origin. Furthermore, the results suggested that the information on size

  3. Molecular Determinants Underlying Binding Specificities of the ABL Kinase Inhibitors: Combining Alanine Scanning of Binding Hot Spots with Network Analysis of Residue Interactions and Coevolution.

    Directory of Open Access Journals (Sweden)

    Amanda Tse

    Full Text Available Quantifying binding specificity and drug resistance of protein kinase inhibitors is of fundamental importance and remains highly challenging due to complex interplay of structural and thermodynamic factors. In this work, molecular simulations and computational alanine scanning are combined with the network-based approaches to characterize molecular determinants underlying binding specificities of the ABL kinase inhibitors. The proposed theoretical framework unveiled a relationship between ligand binding and inhibitor-mediated changes in the residue interaction networks. By using topological parameters, we have described the organization of the residue interaction networks and networks of coevolving residues in the ABL kinase structures. This analysis has shown that functionally critical regulatory residues can simultaneously embody strong coevolutionary signal and high network centrality with a propensity to be energetic hot spots for drug binding. We have found that selective (Nilotinib and promiscuous (Bosutinib, Dasatinib kinase inhibitors can use their energetic hot spots to differentially modulate stability of the residue interaction networks, thus inhibiting or promoting conformational equilibrium between inactive and active states. According to our results, Nilotinib binding may induce a significant network-bridging effect and enhance centrality of the hot spot residues that stabilize structural environment favored by the specific kinase form. In contrast, Bosutinib and Dasatinib can incur modest changes in the residue interaction network in which ligand binding is primarily coupled only with the identity of the gate-keeper residue. These factors may promote structural adaptability of the active kinase states in binding with these promiscuous inhibitors. Our results have related ligand-induced changes in the residue interaction networks with drug resistance effects, showing that network robustness may be compromised by targeted mutations

  4. Identification of the immediate-early genes of white spot syndrome virus.

    Science.gov (United States)

    Li, Fang; Li, Mingyuan; Ke, Wei; Ji, Yongchang; Bian, Xiaofang; Yan, Xiumin

    2009-03-01

    During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. Here we screened white spot syndrome virus (WSSV) IE genes with cycloheximide (CHX)-treated primary culture of crayfish hemocyte and a WSSV genome tiling microarray. Sixteen ORFs, including a known WSSV IE gene (ie1/wsv069), were identified and confirmed by RT-PCR and time course studies. The 16 identified IE proteins contain four proteins (wsv051, wsv069, wsv100, wsv079) with transcription activity, one (wsv083) with Ser/Thr kinase domain and one (wsv249) previously described to function as an ubiquitin E3 ligase. Furthermore, most of the identified WSSV IE genes cluster in a 14 kb genomic region (WSSV China isolate: 36,052 to 50,300 bp). This type of arrangement may facilitate the coordinate control and rapid expression of IE genes.

  5. Temperatures and CH4 mixing ratios near the homopause of the 8 μm north polar hot spot of Jupiter

    Science.gov (United States)

    Kim, Sang Joon; Geballe, Thomas R.; Greathouse, Thomas K.; Yung, Yuk L.; Miller, Steve; Orton, G. S.; Minh, Y. C.

    2017-01-01

    We have derived homopause temperatures of 180-250 K for the 8-μm north-polar hot spot (8NPHS) of Jupiter by fitting CH4 emission models to 3 and 8 μm spectra of the 8NPHS obtained 24 days apart in 2013. From the fits, we find that CH4 mixing ratios at the 8NPHS are consistent with those reported by Kim et al. (2014) in equatorial regions. We propose possible mechanisms to account for the temperature of the 8NPHS homopause, which is relatively cool compared with the temperatures of other auroral regions, including locally-fixed and transient but energetic auroral particle precipitation.

  6. Mapping publication trends and identifying hot spots of research on Internet health information seeking behavior: a quantitative and co-word biclustering analysis.

    Science.gov (United States)

    Li, Fan; Li, Min; Guan, Peng; Ma, Shuang; Cui, Lei

    2015-03-25

    The Internet has become an established source of health information for people seeking health information. In recent years, research on the health information seeking behavior of Internet users has become an increasingly important scholarly focus. However, there have been no long-term bibliometric studies to date on Internet health information seeking behavior. The purpose of this study was to map publication trends and explore research hot spots of Internet health information seeking behavior. A bibliometric analysis based on PubMed was conducted to investigate the publication trends of research on Internet health information seeking behavior. For the included publications, the annual publication number, the distribution of countries, authors, languages, journals, and annual distribution of highly frequent major MeSH (Medical Subject Headings) terms were determined. Furthermore, co-word biclustering analysis of highly frequent major MeSH terms was utilized to detect the hot spots in this field. A total of 533 publications were included. The research output was gradually increasing. There were five authors who published four or more articles individually. A total of 271 included publications (50.8%) were written by authors from the United States, and 516 of the 533 articles (96.8%) were published in English. The eight most active journals published 34.1% (182/533) of the publications on this topic. Ten research hot spots were found: (1) behavior of Internet health information seeking about HIV infection or sexually transmitted diseases, (2) Internet health information seeking behavior of students, (3) behavior of Internet health information seeking via mobile phone and its apps, (4) physicians' utilization of Internet medical resources, (5) utilization of social media by parents, (6) Internet health information seeking behavior of patients with cancer (mainly breast cancer), (7) trust in or satisfaction with Web-based health information by consumers, (8

  7. Bear Creek Valley Floodplain hot spot removal early action characterization field data summary report, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-01

    This report summarizes the field and laboratory efforts as a result of the Bear Creek Floodplain Hot Spot Removal Project Early Action. The purpose of this project was to collect data necessary to assess contaminant levels in the Bear Creek Valley Floodplain and evaluate the risk posed by the sites. This report provides information on the background of the site, characterization of site and field activities, results of field and laboratory data collected, extent and distribution of contamination, and an assessment of the future risk posed by the site.

  8. Study on unified fatigue strength assessment method for welded structure. Hot spot stress evaluating method for various combinations of plate thickness and weld leg length; Yosetsu kozo no toitsutekina hiro kyodo hyokaho ni kansuru kenkyu. Itaatsu to yosetsu ashinaga no kumiawase ni taisuru hot spot oryoku sanshutsu ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Nihei, K.; Inamura, F.; Koe, S. [Kawasaki Heavy Industries, Ltd., Kobe (Japan)

    1996-12-31

    There has been tried to apply unified assessment method using hot spot stress, which is effective to evaluate fatigue strength of fillet welded structures for ships and marine structures. This method can be applied to complicated structures and is independent of welding processes. In this study, first, stress analysis has been conducted for two-dimensional fillet welded joint models with various combinations of plate thickness and weld leg length of general fillet structures by means of boundary element method. Then, critical position, which is not affected by local stress concentration due to bead, was determined from the detailed stress distribution in the vicinity of weld toe. As a result, a general equation has been proposed to estimate the hot spot stress by one-point representative method. Second, the fatigue tests of typical fillet welded joints have been conducted by applying this method. Consequently, it was demonstrated that the unified fatigue strength can be evaluated by the S-N data based on hot spot stress range determined from the proposed equation, independent of structural stress concentration. 22 refs., 14 figs.

  9. Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Juan Hsueh-Fen

    2007-05-01

    Full Text Available Abstract Background White spot syndrome (WSS is a viral disease that affects most of the commercially important shrimps and causes serious economic losses to the shrimp farming industry worldwide. However, little information is available in terms of the molecular mechanisms of the host-virus interaction. In this study, we used an expressed sequence tag (EST approach to observe global gene expression changes in white spot syndrome virus (WSSV-infected postlarvae of Penaeus monodon. Results Sequencing of the complementary DNA clones of two libraries constructed from normal and WSSV-infected postlarvae produced a total of 15,981 high-quality ESTs. Of these ESTs, 46% were successfully matched against annotated genes in National Center of Biotechnology Information (NCBI non-redundant (nr database and 44% were functionally classified using the Gene Ontology (GO scheme. Comparative EST analyses suggested that, in postlarval shrimp, WSSV infection strongly modulates the gene expression patterns in several organs or tissues, including the hepatopancreas, muscle, eyestalk and cuticle. Our data suggest that several basic cellular metabolic processes are likely to be affected, including oxidative phosphorylation, protein synthesis, the glycolytic pathway, and calcium ion balance. A group of immune-related chitin-binding protein genes is also likely to be strongly up regulated after WSSV infection. A database containing all the sequence data and analysis results is accessible at http://xbio.lifescience.ntu.edu.tw/pm/. Conclusion This study suggests that WSSV infection modulates expression of various kinds of genes. The predicted gene expression pattern changes not only reflect the possible responses of shrimp to the virus infection but also suggest how WSSV subverts cellular functions for virus multiplication. In addition, the ESTs reported in this study provide a rich source for identification of novel genes in shrimp.

  10. Validation of the DIFFAL, HPAC and HotSpot Dispersion Models Using the Full-Scale Radiological Dispersal Device (FSRDD) Field Trials Witness Plate Deposition Dataset.

    Science.gov (United States)

    Purves, Murray; Parkes, David

    2016-05-01

    Three atmospheric dispersion models--DIFFAL, HPAC, and HotSpot--of differing complexities have been validated against the witness plate deposition dataset taken during the Full-Scale Radiological Dispersal Device (FSRDD) Field Trials. The small-scale nature of these trials in comparison to many other historical radiological dispersion trials provides a unique opportunity to evaluate the near-field performance of the models considered. This paper performs validation of these models using two graphical methods of comparison: deposition contour plots and hotline profile graphs. All of the models tested are assessed to perform well, especially considering that previous model developments and validations have been focused on larger-scale scenarios. Of the models, HPAC generally produced the most accurate results, especially at locations within ∼100 m of GZ. Features present within the observed data, such as hot spots, were not well modeled by any of the codes considered. Additionally, it was found that an increase in the complexity of the meteorological data input to the models did not necessarily lead to an improvement in model accuracy; this is potentially due to the small-scale nature of the trials.

  11. Presaging critical residues in protein interfaces-web server (PCRPi-W: a web server to chart hot spots in protein interfaces.

    Directory of Open Access Journals (Sweden)

    Joan Segura Mora

    Full Text Available BACKGROUND: It is well established that only a portion of residues that mediate protein-protein interactions (PPIs, the so-called hot spot, contributes the most to the total binding energy, and thus its identification is an important and relevant question that has clear applications in drug discovery and protein design. The experimental identification of hot spots is however a lengthy and costly process, and thus there is an interest in computational tools that can complement and guide experimental efforts. PRINCIPAL FINDINGS: Here, we present Presaging Critical Residues in Protein interfaces-Web server (http://www.bioinsilico.org/PCRPi, a web server that implements a recently described and highly accurate computational tool designed to predict critical residues in protein interfaces: PCRPi. PRCPi depends on the integration of structural, energetic, and evolutionary-based measures by using Bayesian Networks (BNs. CONCLUSIONS: PCRPi-W has been designed to provide an easy and convenient access to the broad scientific community. Predictions are readily available for download or presented in a web page that includes among other information links to relevant files, sequence information, and a Jmol applet to visualize and analyze the predictions in the context of the protein structure.

  12. Using penumbral imaging to measure micrometer size plasma hot spots in Gbar equation of state experiments on the National Ignition Facility

    Energy Technology Data Exchange (ETDEWEB)

    Bachmann, B., E-mail: bachmann2@llnl.gov; Kritcher, A. L.; Benedetti, L. R.; Glenn, S.; Hawreliak, J.; Izumi, N.; Landen, O. L.; Le Pape, S.; Ma, T.; Pérez, F.; Swift, D.; Döppner, T. [Lawrence Livermore National Laboratory, Livermore, California 94550 (United States); Falcone, R. W. [Department of Physics, University of California, Berkeley, California 94720 (United States); Lawrence Berkeley National Laboratory, Berkeley, California 94720 (United States); Kraus, D. [Department of Physics, University of California, Berkeley, California 94720 (United States)

    2014-11-15

    We have developed an experimental platform for absolute equation of state measurements up to Gbar pressures on the National Ignition Facility (NIF) within the Fundamental Science Program. We use a symmetry-tuned hohlraum drive to launch a spherical shock wave into a solid CH sphere. Streaked radiography is the primary diagnostic to measure the density change at the shock front as the pressure increases towards smaller radii. At shock stagnation in the center of the capsule, we observe a short and bright x-ray self emission from high density (∼50 g/cm{sup 3}) plasma at ∼1 keV. Here, we present results obtained with penumbral imaging which has been carried out to characterize the size of the hot spot emission. This allows extending existing NIF diagnostic capabilities for spatial resolution (currently ∼10 μm) at higher sensitivity. At peak emission we find the hot spot radius to be as small as 5.8 +/− 1 μm, corresponding to a convergence ratio of 200.

  13. The research of internet public opinion hot spot mining%网络舆情热点挖掘算法研究与实现

    Institute of Scientific and Technical Information of China (English)

    黄敏

    2012-01-01

    随着网络信息交互及传播的迅速与便捷,有效地对不断涌现的海量互联网信息进行采集并发现网络热点舆情具有十分重要的意义.研究网络舆情的表示及特征,提出采用网络化方法思想,结合Hits算法和PageRank算法进行舆情热点挖掘,并在此基础上设计了网络舆情热点挖掘系统.%With the speedy interaction and spread of the network information, it has great significance to collect emerging massive internet information and discover hot spots of network public opinion. This paper studied the characteristics of network public opinion and designed hot spot mining system on the basis of combination of Hits and PageRank algorithms.

  14. Expression of Thyroid Hormone Responsive SPOT 14 Gene Is Regulated by Estrogen in Chicken (Gallus gallus).

    Science.gov (United States)

    Ren, Junxiao; Xu, Naiyi; Zheng, Hang; Tian, Weihua; Li, Hong; Li, Zhuanjian; Wang, Yanbin; Tian, Yadong; Kang, Xiangtao; Liu, Xiaojun

    2017-08-31

    Thyroid hormone responsive spot 14 (THRSP) is a small nuclear protein that responds rapidly to thyroid hormone. It has been shown that THRSP is abundant in lipogenic tissues such as liver, fat and the mammary gland in mammals. The THRSP gene acts as a key lipogenic activator and can be activated by thyroid hormone triiodothyronine (T3), glucose, carbohydrate and insulin. Here we report that chicken THRSP is also abundant in lipogenic tissues including the liver and the abdominal fat, and its expression levels increased with sex maturation and reached the highest level at the peak of egg production. Structure analysis of the THRSP gene indicates that there is a conscious estrogen response element (ERE) located in the -2390 - -2402 range of the gene promoter region. Further studies by ChIP-qPCR proved that the ERα interacts with the putative ERE site. In addition, THRSP was significantly upregulated (P estrogen and is involved in the estrogen regulation network in chicken.

  15. Four kinds of ENU-induced white spot mice and chromosome locations of the mutant genes

    Institute of Scientific and Technical Information of China (English)

    WU Baojin; MAO Huihua; SHAO Yixiang; XUE Zhengfeng; LI Houda

    2003-01-01

    Phenotype-driven is the name for an approach used to study gene functions through mutagenesis, location and cloning of the mutant gene. In this study, 150 male C57BL/6J(B6) mice were treated with ENU and reproduced a total offspring of 3860. Of these descendants, 210 exhibited mutation phenotypes by screening, and more than 10 of them are hereditable. Four kinds of mutant mice, named Wbct, W-1Bao, W-2Bao, and W-3Bao, showed dominant hereditary white spot mutation with partial albinism on their belly, distal limbs and tail terminal. To map these mutant genes, 39 microsatellites, equally distributed on the mouse genome and with difference between B6 and DBA/2J (D2), were selected to scan the genome after discrimination of the white spots in the F2 mice [(B6×D2)×D2]. It is found that, the log odds score (LODS) between W-1Bao and D5Mit168 is 0.56, and the LODS of W-1Bao and D5Mit352 is 4.47. With the gradual application of microsatellites D5Mit290, D5Mit312, D5Mit308 and D5Mit356 that are close to the mutant gene, and the number of F2 mice going up to 537, the mutant W-1Bao is located between D5Mit356 and D5Mit308 on chromosome 5, about 42.19 cM from the centromere. In the same way, W-2Bao and W-3Bao are mapped nearby W-1Bao, and Wbct is located on chromosome 1, about 41.6 cM from the centromere. After searching for the mouse genome database (MGD) and performing a one-by-one study of all genes located on chromosome subregion, it is believed that the kit gene is an excellent candidate for the white spot mutations of W-1Bao, W-2Bao and W-3Bao.

  16. Tsw gene-based resistance is triggered by a functional RNA silencing suppressor protein of the Tomato spotted wilt virus

    NARCIS (Netherlands)

    Ronde, de D.; Butterbach, P.B.E.; Lohuis, H.; Hedil, M.; Lent, van J.W.M.; Kormelink, R.J.M.

    2013-01-01

    As a result of contradictory reports, the avirulence (Avr) determinant that triggers Tsw gene-based resistance in Capsicum annuum against the Tomato spotted wilt virus (TSWV) is still unresolved. Here, the N and NSs genes of resistance-inducing (RI) and resistance-breaking (RB) isolates were cloned

  17. Using SNP genetic markers to elucidate the linkage of the Co-34/Phg-3 anthracnose and angular leaf spot resistance gene cluster with the Ur-14 resistance gene

    Science.gov (United States)

    The Ouro Negro common bean cultivar contains the Co-34/Phg-3 gene cluster that confers resistance to the anthracnose (ANT) and angular leaf spot (ALS) pathogens. These genes are tightly linked on chromosome 4. Ouro Negro also has the Ur-14 rust resistance gene, reportedly in the vicinity of Co- 34; ...

  18. Doubling the efficiency of third harmonic generation by positioning ITO nanocrystals into the hot-spot of plasmonic gap-antennas.

    Science.gov (United States)

    Metzger, Bernd; Hentschel, Mario; Schumacher, Thorsten; Lippitz, Markus; Ye, Xingchen; Murray, Christopher B; Knabe, Bastian; Buse, Karsten; Giessen, Harald

    2014-05-14

    We incorporate dielectric indium tin oxide nanocrystals into the hot-spot of gold nanogap-antennas and perform third harmonic spectroscopy on these hybrid nanostructure arrays. The combined system shows a 2-fold increase of the radiated third harmonic intensity when compared to bare gold antennas. In order to identify the origin of the enhanced nonlinear response we perform finite element simulations of the nanostructures, which are in excellent agreement with our measurements. We find that the third harmonic signal enhancement is mainly related to changes in the linear optical properties of the plasmonic antenna resonances when the ITO nanocrystals are incorporated. Furthermore, the dominant source of the third harmonic is found to be located in the gold volume of the plasmonic antennas.

  19. An outdoor investigation of the absorption degradation of single-junction amorphous silicon photovoltaic module due to localized heat/hot spot formation

    Indian Academy of Sciences (India)

    Osayemwenre Gilbert O; Meyer Edson L; Mamphweli Sampson

    2016-04-01

    This paper investigates the absorbance degradation of single-junction amorphous silicon (a-Si:H) photovoltaic (PV) module, due to the presence of localized heat. The decrease in optical density is a huge challenge due to the long-term degradation of PV modules. The reduction in solar cell optical density causes a decline in its conversion efficiency. This decreases the photogenerating current, hence reduces the effective efficiency of the PV device. An infrared thermography was used for mapping the module temperature profile. Fourier transform infrared spectroscopy (FTIR) was used for the absorption characterization. The rationale behind the outdoor deployment was to deduce a practical effect of hot spot formation on the module’s absorption ability.The results show a direct correlation between localized heat and the absorption degradation.

  20. Identification of 'carbon hot-spots' and quantification of GHG intensities in the biodiesel supply chain using hybrid LCA and structural path analysis.

    Science.gov (United States)

    Acquaye, Adolf A; Wiedmann, Thomas; Feng, Kuishang; Crawford, Robert H; Barrett, John; Kuylenstierna, Johan; Duffy, Aidan P; Koh, S C Lenny; McQueen-Mason, Simon

    2011-03-15

    It is expected that biodiesel production in the EU will remain the dominant contributor as part of a 10% minimum binding target for biofuel in transportation fuel by 2020 within the 20% renewable energy target in the overall EU energy mix. Life cycle assessments (LCA) of biodiesel to evaluate its environmental impacts have, however, remained questionable, mainly because of the adoption of a traditional process analysis approach resulting in system boundary truncation and because of issues regarding the impacts of land use change and N(2)O emissions from fertilizer application. In this study, a hybrid LCA methodology is used to evaluate the life cycle CO(2) equivalent emissions of rape methyl ester (RME) biodiesel. The methodology uses input-output analysis to estimate upstream indirect emissions in order to complement traditional process LCA in a hybrid framework. It was estimated that traditional LCA accounted for 2.7 kg CO(2)-eq per kg of RME or 36.6% of total life cycle emissions of the RME supply chin. Further to the inclusion of upstream indirect impacts in the LCA system (which accounted for 23% of the total life cycle emissions), emissions due to direct land use change (6%) and indirect land use change (16.5%) and N(2)O emissions from fertilizer applications (17.9%) were also calculated. Structural path analysis is used to decompose upstream indirect emissions paths of the biodiesel supply chain in order to identify, quantify, and rank high carbon emissions paths or 'hot-spots' in the biodiesel supply chain. It was shown, for instance, that inputs from the 'Other Chemical Products' sector (identified as phosphoric acid, H(3)PO(4)) into the biodiesel production process represented the highest carbon emission path (or hot-spot) with 5.35% of total upstream indirect emissions of the RME biodiesel supply chain.

  1. Characterizing the Hot Spots Involved in RON-MSPβ Complex Formation Using In Silico Alanine Scanning Mutagenesis and Molecular Dynamics Simulation

    Directory of Open Access Journals (Sweden)

    Omid Zarei

    2017-04-01

    Full Text Available Purpose: Implication of protein-protein interactions (PPIs in development of many diseases such as cancer makes them attractive for therapeutic intervention and rational drug design. RON (Recepteur d’Origine Nantais tyrosine kinase receptor has gained considerable attention as promising target in cancer therapy. The activation of RON via its ligand, macrophage stimulation protein (MSP is the most common mechanism of activation for this receptor. The aim of the current study was to perform in silico alanine scanning mutagenesis and to calculate binding energy for prediction of hot spots in protein-protein interface between RON and MSPβ chain (MSPβ. Methods: In this work the residues at the interface of RON-MSPβ complex were mutated to alanine and then molecular dynamics simulation was used to calculate binding free energy. Results: The results revealed that Gln193, Arg220, Glu287, Pro288, Glu289, and His424 residues from RON and Arg521, His528, Ser565, Glu658, and Arg683 from MSPβ may play important roles in protein-protein interaction between RON and MSP. Conclusion: Identification of these RON hot spots is important in designing anti-RON drugs when the aim is to disrupt RON-MSP interaction. In the same way, the acquired information regarding the critical amino acids of MSPβ can be used in the process of rational drug design for developing MSP antagonizing agents, the development of novel MSP mimicking peptides where inhibition of RON activation is required, and the design of experimental site directed mutagenesis studies.

  2. Optimization of Resistance Spot Weld Condition for Single Lap Joint of Hot Stamped 22MnB5 by Taking Heating Temperature and Heating Time into Consideration

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Hong Seok; Kim, Byung Min; Park, Geun Hwan [Pusan National University, Busan (Korea, Republic of); Lim, Woo Seung [Keimyung University, Daegu (Korea, Republic of)

    2010-10-15

    In this study, optimization of the process parameters of the resistance spot welding of a sheet of aluminum-coated boron alloyed steel, 22MnB5, used in hot stamping has been performed by a Taguchi method to increase the strength of the weld joint. The process parameters selected were current, electrode force, and weld time. The heating temperature and heating time of 22MnB5 are considered to be noise factors. It was known that the variation in the thickness of the intermetallic compound layer between the aluminum-coated layer and the substrate, which influences on the formation of nugget, was generated due to the difference of diffusion reaction according to heating conditions. From the results of spot weld experiment, the optimum weld condition was determined to be when the current, electrode force, and weld time were 8kA, 4kN, and 18 cycles, respectively. The result of a test performed to verify the optimized weld condition showed that the tensile strength of the weld joint was over 32kN, which is considerably higher than the required strength, i.e., 23kN.

  3. Abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China.

    Science.gov (United States)

    Song, Zhao-Qi; Wang, Li; Wang, Feng-Ping; Jiang, Hong-Chen; Chen, Jin-Quan; Zhou, En-Min; Liang, Feng; Xiao, Xiang; Li, Wen-Jun

    2013-09-01

    It has been suggested that archaea carrying the accA gene, encoding the alpha subunit of the acetyl CoA carboxylase, autotrophically fix CO2 using the 3-hydroxypropionate/4-hydroxybutyrate pathway in low-temperature environments (e.g., soils, oceans). However, little new information has come to light regarding the occurrence of archaeal accA genes in high-temperature ecosystems. In this study, we investigated the abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China, using DNA- and RNA-based phylogenetic analyses and quantitative polymerase chain reaction. The results showed that archaeal accA genes were present and expressed in the investigated Yunnan hot springs with a wide range of temperatures (66-96 °C) and pH (4.3-9.0). The majority of the amplified archaeal accA gene sequences were affiliated with the ThAOA/HWCG III [thermophilic ammonia-oxidizing archaea (AOA)/hot water crenarchaeotic group III]. The archaeal accA gene abundance was very close to that of AOA amoA gene, encoding the alpha subunit of ammonia monooxygenase. These data suggest that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway.

  4. Internet Public Opinion Hot Spot Mining Base on Complex Network Theory%基于复杂网络方法的舆情热点挖掘

    Institute of Scientific and Technical Information of China (English)

    黄敏; 胡学钢

    2011-01-01

    This paper focused on the Internet public opinion hot spot mining based on the complex network theory and methods. Internet public opinion analysis techniques can be divided into content-based analytical methods and analyses based on data mining methods. Both are traditional technology and have no relation with the network. This paper gave the idea of public opinion analyses and proposed a network-based approach to resolve network problems. The public opinion web pages could be represented as nodes of complex networks and the sides were links of those pages. The public opinion network meets the complex network characteristics. Based on Hits algorithm and PageRank algorithm, we can find out the most popular public opinions in the network, which showed a hot public opinion. With a data set from Wikipedia, experimental results show that both methods can identify hot spots of public opinion networks. Because PageRank method focuses on the impact of link nodes and Hits algorithm focus on the core degree of the nodes, the mining results have some difference. We can combine the mining results of the two methods according to the field characteristics to get the Internet public opinion hot spots.%研究使用复杂网络理论和算法的网络舆情热点提取.网络舆情分析技术可以分为基于内容的分析方法和基于数据挖掘的分析方法,二者均是采用传统技术处理网络舆情,并未结合网络特性.针对上述问题,开拓了舆情分析的思路,提出采用网络化方法处理网络问题的思想,将舆情网络的页面视为节点,链接关系作为边,构建符合复杂网络特征的舆情传播网络.使用PageRank算法和Hits算法,可以挖掘出舆情网络中的重要节点,进而发现舆情热点.以维基百科数据为仿真数据集,采用新提出的方法,可以挖掘相关领域的热点条目.仿真结果表明,两种方法均可以完成网络舆情热点的发掘工作,从复杂网络的角度处理网络舆情

  5. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot.

    Science.gov (United States)

    Armesto, Cecilia; Maia, Fernanda Gonçalves Martins; de Abreu, Mário Sobral; Figueira, Antonia Dos Reis; da Silva, Bruno Marques; Monteiro, Fernando Pereira

    2012-07-01

    Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host × pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.

  6. Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

    Directory of Open Access Journals (Sweden)

    Cecilia Armesto

    2012-09-01

    Full Text Available Blister spot (Colletotrichum gloeosporioides is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.

  7. Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae.

    Directory of Open Access Journals (Sweden)

    Chunxiao Yang

    Full Text Available Quantitative real-time PCR (qRT-PCR is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory. In this study, ten housekeeping genes, including beta-actin (Actin , elongation factor 1 α (EF1A , glyceralde hyde-3-phosphate dehydrogenase (GAPDH , ribosomal protein L13 (RPL13 , ribosomal protein 49 (RP49 , α-tubulin (Tubulin , vacuolar-type H+-ATPase (v-ATPase , succinate dehydrogenase subunit A (SDHA , 28S ribosomal RNA (28S , and 18S ribosomal RNA (18S from the two-spotted spider mite, Tetranychus urticae, were selected as the candidate reference genes. Four algorithms, geNorm, Normfinder, BestKeeper, and the ΔCt method, were used to evaluate the performance of these candidates as endogenous controls across different developmental stages. In addition, RefFinder, which integrates the above-mentioned software tools, provided the overall ranking of the stability/suitability of these candidate reference genes. Among them, PRL13 and v-ATPase were the two most stable housekeeping genes across different developmental stages. This work is the first step toward establishing a standardized qRT-PCR analysis in T. urticae following the MIQE guideline. With the recent release of the T. urticae genome, results from this study provide a critical piece for the subsequent genomics and functional genomics research in this emerging model system.

  8. Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus.

    Science.gov (United States)

    Tsai, M F; Lo, C F; van Hulten, M C; Tzeng, H F; Chou, C M; Huang, C J; Wang, C H; Lin, J Y; Vlak, J M; Kou, G H

    2000-11-10

    The causative agent of white spot syndrome (WSS) is a large double-stranded DNA virus, WSSV, which is probably a representative of a new genus, provisionally called Whispovirus. From previously constructed WSSV genomic libraries of a Taiwan WSSV isolate, clones with open reading frames (ORFs) that encode proteins with significant homology to the class I ribonucleotide reductase large (RR1) and small (RR2) subunits were identified. WSSV rr1 and rr2 potentially encode 848 and 413 amino acids, respectively. RNA was isolated from WSSV-infected shrimp at different times after infection and Northern blot analysis with rr1- and rr2-specific riboprobes found major transcripts of 2.8 and 1.4 kb, respectively. 5' RACE showed that the major rr1 transcript started at a position of -84 (C) relative to the ATG translational start, while transcription of the rr2 gene started at nucleotide residue -68 (T). A consensus motif containing the transcriptional start sites for rr1 and rr2 was observed (TCAc/tTC). Northern blotting and RT-PCR showed that the transcription of rr1 and rr2 started 4-6 h after infection and continued for at least 60 h. The rr1 and rr2 genes thus appear to be WSSV "early genes."

  9. Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus.

    Science.gov (United States)

    Chen, Li-Li; Wang, Han-Ching; Huang, Chiu-Jung; Peng, Shao-En; Chen, Yen-Gu; Lin, Shin-Jen; Chen, Wei-Yu; Dai, Chang-Feng; Yu, Hon-Tsen; Wang, Chung-Hsiung; Lo, Chu-Fang; Kou, Guang-Hsiung

    2002-09-15

    The white spot syndrome virus DNA polymerase (DNA pol) gene (WSSV dnapol) has already been tentatively identified based on the presence of highly conserved motifs, but it shows low overall homology with other DNA pols and is also much larger (2351 amino acid residues vs 913-1244 aa). In the present study we perform a transcriptional analysis of the WSSV dnapol gene using the total RNA isolated from WSSV-infected shrimp at different times after infection. Northern blot analysis with a WSSV dnapol-specific riboprobe found a major transcript of 7.5 kb. 5'-RACE revealed that the major transcription start point is located 27 nucleotides downstream of the TATA box, at the nucleotide residue A within a CAGT motif, one of the initiator (Inr) motifs of arthropods. In a temporal expression analysis using differential RT-PCR, WSSV dnapol transcripts were detected at low levels at 2-4 h.p.i., increased at 6 h.p.i., and remained fairly constant thereafter. This is similar to the previously reported transcription patterns for genes encoding the key enzyme of nucleotide metabolism, ribonucleotide reductase. Phylogenetic analysis showed that the DNA pols from three different WSSV isolates form an extremely tight cluster. In addition, similar to an earlier phylogenetic analysis of WSSV protein kinase, the phylogenetic tree of viral DNA pols further supports the suggestion that WSSV is a distinct virus (likely at the family level) that does not belong to any of the virus families that are currently recognized.

  10. A new assessment of combined geothermal electric generation and desalination in western Saudi Arabia: targeted hot spot development

    KAUST Repository

    Missimer, Thomas M.

    2014-07-17

    High heat flow associated with the tectonic spreading of the Red Sea make western Saudi Arabia a region with high potential for geothermal energy development. The hydraulic properties of the Precambrian-age rocks occurring in this region are not conducive to direct production of hot water for heat exchange, which will necessitate use of the hot dry rock (HDR) heat harvesting method. This would require the construction of coupled deep wells; one for water injection and the other for steam recovery. There are some technological challenges in the design, construction, and operation of HDR geothermal energy systems. Careful geotechnical evaluation of the heat reservoir must be conducted to ascertain the geothermal gradient at the chosen site to allow pre-design modeling of the system for assessment of operational heat flow maintenance. Also, naturally occurring fractures or faults must be carefully evaluated to make an assessment of the potential for induced seismicity. It is anticipated that the flow heat exchange capacity of the system will require enhancement by the use of horizontal drilling and hydraulic fracturing in the injection well with the production well drilled into the fracture zone to maximum water recovery efficiency and reduce operating pressure. The heated water must be maintained under pressure and flashed to steam at surface to produce to the most effective energy recovery. Most past evaluations of geothermal energy development in this region have been focused on the potential for solely electricity generation, but direct use of produced steam could be coupled with thermally driven desalination technologies such as multi-effect distillation, adsorption desalination, and/or membrane distillation to provide a continuous source of heat to allow very efficient operation of the plants. © 2014 © 2014 Balaban Desalination Publications. All rights reserved.

  11. Characterisation of class II B MHC genes from a ratite bird, the little spotted kiwi (Apteryx owenii).

    Science.gov (United States)

    Miller, Hilary C; Bowker-Wright, Gemma; Kharkrang, Marie; Ramstad, Kristina

    2011-04-01

    Major histocompatibility complex (MHC) genes are important for vertebrate immune response and typically display high levels of diversity due to balancing selection from exposure to diverse pathogens. An understanding of the structure of the MHC region and diversity among functional MHC genes is critical to understanding the evolution of the MHC and species resilience to disease exposure. In this study, we characterise the structure and diversity of class II MHC genes in little spotted kiwi Apteryx owenii, a ratite bird representing the basal avian lineage (paleognaths). Results indicate that little spotted kiwi have a more complex MHC structure than that of other non-passerine birds, with at least five class II MHC genes, three of which are expressed and likely to be functional. Levels of MHC variation among little spotted kiwi are extremely low, with 13 birds assayed having nearly identical MHC genotypes (only two genotypes containing four alleles, three of which are fixed). These results suggest that recent genetic drift due to a species-wide bottleneck of at most seven birds has overwhelmed past selection for high MHC diversity in little spotted kiwi, potentially leaving the species highly susceptible to disease.

  12. Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene

    Directory of Open Access Journals (Sweden)

    Yu Li

    2005-08-01

    Full Text Available Abstract Background White spot syndrome virus (WSSV is an important pathogen of the penaeid shrimp with high mortalities. In previous reports, Orf427 of WSSV is characterized as one of the three major latency-associated genes of WSSV. Here, we were interested to analyze the promoter of orf427 and its expression during viral pathogenesis. Results in situ hybridization revealed that orf427 was transcribed in all the infected tissues during viral lytic infection and the translational product can be detected from the infected shrimp. A time-course RT-PCR analysis indicated that transcriptional products of orf427 could only be detected after 6 h post virus inoculation. Furthermore, a baculovirus-mediated promoter analysis indicated that the promoter of orf427 failed to express the EGFP reporter gene in both insect SF9 cells and primary shrimp cells. Conclusion Our data suggested that latency-related orf427 might not play an important role in activating virus replication from latent phase due to its late transcription during the lytic infection.

  13. Ethoxyresorufin-o-deethylase induction potency in sediment samples from rivers Lepenica and Morawa. Surrounding area of Kragujevac ''hot spot''

    Energy Technology Data Exchange (ETDEWEB)

    Zoric, S.; Andric, N.; Kovacevic, R. [Novi Sad Univ. (Yugoslavia). Faculty of Sciences, Dept. of Biology and Ecology; Sudji, J. [Institute of Occupational Health, Novi Sad (Yugoslavia); Klanova, J. [Masaryk Univ., Brno (Czech Republic). Dept. of Environmental Chemistry and Ecotoxicology; Jovetic, S.; Vojinovic-Miloradov, M. [Novi Sad Univ. (Yugoslavia). Faculty of Sciences, Dept of Chemistry

    2004-09-15

    Introduction Activation of aryl hydrocarbon receptor (AhR) and induction of CYP1A1 izozyme, whose activity can be determined by measuring activity of 7-ethoxyresorufin-o-deethylase (EROD) in various species and test systems, are well known bioanalyses for assessment of toxicity of persistent toxic compounds (PTCs). Whereas analytical methods determine the concentrations of known substances, bioanalyses with EROD as an end point may also detect the joint activities of nonanalyzed EROD inducing compounds in environmental samples. The main environmental effects of the bombardments (in 1999) of factory Zastava-Kragujevac were damaged transformers which contained Pyralene oil, leaking of several tons of PCBs into the rivers Zdraljica, Lepenica and Morava, and contamination of groundwater by PCBs and heavy metals. Up to 2,500 kg of oil containing PCBs was released into the environment. Extremely high levels of PCBs were detected in water ecosystems in the area of accident - in the river Lepenica PCB levels were 400-1000mg/l of water. As a consequence of explosions, fires, and PCB combustions, very high levels of PCDDs/Fs were found in soil samples from that area (up to 100,000ng I-TEQ/kg) few months after bombardments. Very high levels of PCBs and PCDDs/Fs were also found in samples taken around the transformers of the power plant (70-74 g/kg of PCBs and 10,200 ng I-TEQ/kg of PCDDs/Fs). Right after spillage there was a flood wave from the river Lepenica that caused transport and sedimentation of the pollutants along nearby agricultural area. In 2000, UNEP/UNOPS Clean-up Project YUG 00-R71 was performed for clean up action of environmental damage of hot spots in SCG. However, further investigations which covered hot spot Kragujevac as a zone possibly contaminated with PCBs revealed that there are still ppm concentrations of PCBs as determined by GC/ECD. In this study we explored if these contaminants still in sediments from rivers Lepenica and Morava. Sediment samples

  14. 国内网络舆情研究热点与趋势分析%Domestic Internet Public Opinion Research Hot Spot and Trend

    Institute of Scientific and Technical Information of China (English)

    卢卉; 黄建忠

    2011-01-01

    目的 了解国内网络舆情热点,提高舆情监测能力,为政府及相关部门决策提供服务.方法 通过下载CNKI收录的网络舆情论文(2005 - 2010年),借助于统计软件与科学计量方法对近6年来我国网络舆情的研究状况进行分析.结果 研究的热点问题为文本挖掘、引导、高校、突发事件、模型、舆情分析、预警、指标体系等;舆情研究已进入了发展阶段,形成了一批高产作者和出名的研究机构,部分阶段性研究成果已在网络舆情监测系统中得到应用.结论 舆情研究已进入了发展阶段,舆情监测工作应从实际需求出发,实时研究舆情发展趋势.%Objective To provide services for the decision meking of government and relevant departments by understanding the hot spot of the domestic internet public opinion and improving the monitoring capacity of public opinion. Methods Downloaded the domestic internet public opinion papers in CNKI (2005 -2010), with the situation of domestic internet public opinion in the past six years was analyzed by means of statistical software and scientific measurement methods. Results The research hot spot topics were the text mining, guide, college, emergency, models, public opinion analysis, early warning, indicator system and, etc. Public opinion research has entered the developing stage, with a group of high production authors and famous research institutions has formed. Part of initial findings of public opinion research has been applied in network monitoring system. Conclusion Public opinion research has entered the developing stage. Public opinion mornitoring should based on the actual demand to conduct real - time study of the developing trend.

  15. A Look into the Melting Pot: The mecC-Harboring Region Is a Recombination Hot Spot in Staphylococcus stepanovicii

    OpenAIRE

    Torsten Semmler; Harrison, Ewan M.; Antina Lübke-Becker; Ulrich, Rainer G.; Wieler, Lothar H; Sebastian Guenther; Ivonne Stamm; Anne-Merethe Hanssen; Holmes, Mark A.; Szilvia Vincze; Birgit Walther

    2016-01-01

    Publisher's version, source: http://dx.doi.org/10.1371/journal.pone.0147150. INTRODUCTION Horizontal gene transfer (HGT) is an important driver for resistance- and virulence factor accumulation in pathogenic bacteria such as Staphylococcus aureus. METHODS Here, we have investigated the downstream region of the bacterial chromosomal attachment site (attB) for the staphylococcal cassette chromosome mec (SCCmec) element of a commensal mecC-positive Staphylococcus stepanovicii stra...

  16. Insights into the origin and distribution of biodiversity in the Brazilian Atlantic forest hot spot: a statistical phylogeographic study using a low-dispersal organism.

    Science.gov (United States)

    Álvarez-Presas, M; Sánchez-Gracia, A; Carbayo, F; Rozas, J; Riutort, M

    2014-06-01

    The relative importance of the processes that generate and maintain biodiversity is a major and controversial topic in evolutionary biology with large implications for conservation management. The Atlantic Forest of Brazil, one of the world's richest biodiversity hot spots, is severely damaged by human activities. To formulate an efficient conservation policy, a good understanding of spatial and temporal biodiversity patterns and their underlying evolutionary mechanisms is required. With this aim, we performed a comprehensive phylogeographic study using a low-dispersal organism, the land planarian species Cephaloflexa bergi (Platyhelminthes, Tricladida). Analysing multi-locus DNA sequence variation under the Approximate Bayesian Computation framework, we evaluated two scenarios proposed to explain the diversity of Southern Atlantic Forest (SAF) region. We found that most sampled localities harbour high levels of genetic diversity, with lineages sharing common ancestors that predate the Pleistocene. Remarkably, we detected the molecular hallmark of the isolation-by-distance effect and little evidence of a recent colonization of SAF localities; nevertheless, some populations might result from very recent secondary contacts. We conclude that extant SAF biodiversity originated and has been shaped by complex interactions between ancient geological events and more recent evolutionary processes, whereas Pleistocene climate changes had a minor influence in generating present-day diversity. We also demonstrate that land planarians are an advantageous biological model for making phylogeographic and, particularly, fine-scale evolutionary inferences, and propose appropriate conservation policies.

  17. Hot and Cool Spots of Primary Production, Respiration and 15N Nitrate and Ammonium Uptake: Spatial Heterogeneity in Tropical Streams and Rivers

    Science.gov (United States)

    Dodds, W. K.; Tromboni, F.; Neres-Lima, V.; Zandoná, E.; Moulton, T. P.

    2016-12-01

    While whole-stream measures of metabolism and uptake have become common methods to characterize biogeochemical transport and processing, less is known about how nitrogen (N) uptake, gross primary production (GPP) and ecosystem respiration (ER) covary among different stream substrata as smaller scales. We measured 15N ammonium and nitrate uptake seperately, and GPP and ER of ecosystem compartments (leaves, epilithon, sand-associated biota and macrophytes) in closed circulating chambers in three streams/ rivers of varied size. The streams drain pristine Brazilian Atlantic Rainforest watersheds and are all within a few km of eachother. The smallest stream had dense forest canopy cover; the largest river was almost completely open. GPP could not be detected in the closed canopy stream. Epilithon (biofilms on rocks) was a dominant compartment for GPP and N uptake in the two open streams, and macrophytes rivaled epilithon GPP and N uptake rates in the most open stream. Even though leaves covered only 1-3% of the stream bottom, they could account for around half of all the ER in the streams but almost no N uptake. Sand had minimal rates of N uptake, GPP and R associated with it in all streams due to relatively low organic material content. The data suggest that N uptake, GPP and ER of different substrata are not closely linked over relatively small spatial (dm) scales, and that different biogeochemical processes may map to different hot and cool spots for ecosystem rates.

  18. Transformation from "Carbon Valley" to a "Post-Carbon Society" in a Climate Change Hot Spot: the Coalfields of the Hunter Valley, New South Wales, Australia

    Directory of Open Access Journals (Sweden)

    Geoffrey R. Evans

    2008-06-01

    Full Text Available This paper examines the possibilities for transformation of a climate-change hot spot - the coal-producing Hunter Region of New South Wales, Australia - using complex adaptive systems (CAS theory. It uses CAS theory to understand the role of coal in the region's history and efforts to strengthen the ecological, economic, and social resilience of the region's coal industry in the face of demands for a shift from fossil fuel dependency to clean, renewable energy and genuine resilience and sustainability. It uses CAS theory to understand ways in which the resilience of two alternative futures, labeled "Carbon Valley" and "Post-Carbon Society" (Heinberg 2004, might evolve. The paper discusses ways in which changes implemented through the efforts of local communities at local, smaller scales of the nested systems seek to influence the evolution of adaptive cycles of the system at the local, national, and global scales. It identifies the influences of "attractors," defined as factors driving the evolution of the system, that are influential across the panarchy. These include climate change threats, markets, regulatory regimes, political alliances, and local concerns about the environmental and social impacts of the Hunter's coal dependency. These factors are weakening the apparent resilience of the coal industry, which is being propped up by the coal industry corporations, labor unions, and governments to maintain coal dependency in the Carbon Valley. Moreover, they are creating an alternative basin of attraction in which a Post-Carbon Society might emerge from the system's evolutionary processes.

  19. Biomagnetic monitoring of particulate matter (PM through leaves of an invasive alien plant Lantana camara in an Indo-Burma hot spot region

    Directory of Open Access Journals (Sweden)

    Prabhat Kumar Rai

    2016-03-01

    Full Text Available Present study was performed in urban forests of Aizawl, Mizoram, North East India falling under an Indo-Burma hot spot region of existing ecological relevance and pristine environment. Phyto-sociolology of invasive weeds has been performed and results revealed that Lantana camara was the most dominant invasive weed. Further, the air quality studies revealed high suspended particulate matter (SPM as well as respirable suspended particulate matter (RSPM in ambient air of Aizawl, Mizoram, North East India. Bio-magnetic monitoring through plant leaves has been recognised as recent thrust area in the field of particulate matter (PM science. We aimed to investigate that whether magnetic properties of Lantana camara leaves may act as proxy of PM pollution and hence an attempt towards it's sustainable management. Magnetic susceptibility (χ, Anhyste reticremanent magnetization (ARM and Saturation isothermal remanent magnetization (SIRM of Lantana camara plant leaves were assessed and concomitantly correlated these magnetic properties with ambient PM in order to screen this invasive plant which may act as proxy for ambient PM concentrations. Results revealed high χ, ARM, SIRM of Lantana camara leaves and moreover, these parameters were having significant and positive correlation with ambient SPM as well as RSPM. Therefore, present study recommended the use of Lantana camara as bio-magnetic monitor which may further have sustainable management implications of an invasive plant.

  20. Hot spots of sentinel node biopsy in breast cancer%乳腺癌前哨淋巴结活组织检查的研究

    Institute of Scientific and Technical Information of China (English)

    王永胜

    2009-01-01

    乳腺癌前哨淋巴结(SLN)活检代表着乳腺癌外科治疗的发展水平,但其广泛开展可能超越了已有的证据支持.目前乳腺癌SLN活检研究的热点包括SLN微转移的预后意义、SLN阳性患者非SLN(nSLN)转移预测及SLN术中分子诊断等,结合2008年圣·安东尼奥乳腺癌会议的最新研究结果对其进行述评.%Sentinel lymph node biopsy represents the state of the art of breast surgery, but its widely application might surpass the support of available evidences. The hot spots of sentinel lymph node biopsy in breast cancer include the prognosis of micrometastases in sentinel nodes, the prediction of non-sentinel nodes metastases in positive sentinel nodes patients, and the intraoperative molecular diagnosis of sentinel nodes.These are discussed accompanying with the updated results from the 31st SABCS held in December, 2008.

  1. Depth to Curie temperature or bottom of the magnetic sources in the volcanic zone of la Réunion hot spot

    Science.gov (United States)

    Gailler, Lydie-Sarah; Lénat, Jean-François; Blakely, Richard J.

    2016-09-01

    We present an innovative study to generalize Curie Point Depth (CPD) determinations at the scale of oceanic volcanic islands, an approach which has previously focused largely on continental areas. In order to determine the validity of this technique in oceanic environments, we first tested the approach on sets of sea-floor-spreading anomalies. Assuming that magnetic anomalies are concentrated within the oceanic crust and uppermost mantle, the Curie depth should deepen as oceanic lithosphere increases in age and thickness away from spreading centers. The calculated depths to the magnetic bottom are in agreement with this general pattern. On the basis of this test, we then applied the method to La Réunion Island and surrounding oceanic lithosphere. The calculated extent of magnetic sources lies at depths between 10 and 30 km and exhibits a complex topography, presumably caused by a combination of various magmatic and tectonic lithospheric structures. These calculations indicate that magnetic sources extend well below the crust-mantle interface at this location. To the first order, the bottom of the magnetic surface shallows beneath Réunion and Mauritius Islands due to the thermal effect of the hot spot, and deepens away from La Réunion edifice. On the scale of the Mascarene Basin, several discontinuities in the CPD correlate well with major fracture zones.

  2. Land Use Change and Agricultural Land Fragmentation due to Anthropogenic Activities in an Hot Spot Area: A Case Study for Thrace Region of Turkey

    Science.gov (United States)

    Altürk, Bahadır; Konukcu, Fatih

    2017-04-01

    Agricultural lands that supply food, energy and ecosystem services for human life have been lost due to anthropogenic activities such as construction of roads, urban and industry areas. The significant reasons for the increase of artificial surfaces were poorly planned economic decisions by the government and internal migration due to this poorly planning. Unplanned urban sprawl also give rise to land fragmentation. Fragmentation of agricultural land affects both the agricultural production capacity and rural sustainable employment. In this study: i) Land use changes between 1990-2014 period were assessed using remotely sensed data and ii) Spatial and temporal agricultural land fragmentation were investigated using landscape pattern indice (effective mesh size), Morphological Spatial Pattern Analysis (MSPA) and Entropy method for 25 years period. The selected"hot spot" study area is located on east Thrace region of Turkey, being the service and industrial development zone where agricultural activities, water resources and natural habitat have been damaged due to rapid urban and industrial development for about 25 years. The results showed that agricultural lands decreased 6.44%, urban areas increased 111.68% and industry areas increased 251.19% during this 25 years period. Additionally, fragmentation analyses demonstrated that core agricultural areas sharply decreased and relative fragmentation (effective mesh size) increased from 50.68% to 56.77% during 1990 and 2014.

  3. Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Huang, Xiaohong; Cai, Jia; Yan, Yang; Guo, Chuanyu; Qin, Qiwei

    2014-01-01

    The lysosomal cysteine protease cathepsin B of papain family is a key regulator and signaling molecule that involves in various biological processes, such as the regulation of apoptosis and activation of virus. In the present study, cathepsin B gene (Ec-CB) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-CB cDNA was composed of 1918 bp and encoded a polypeptide of 330 amino acids with higher identities to cathepsin B of teleosts and mammalians. Ec-CB possessed typical cathepsin B structural features including an N-terminal signal peptide, the propeptide region and the cysteine protease domain which were conserved in other cathepsin B sequences. Phylogenetic analysis revealed that Ec-CB was most closely related to Lutjanus argentimaculatus. RT-PCR analysis showed that Ec-CB transcript was expressed in all the examined tissues which abundant in spleen, kidney and gill. After challenged with Singapore grouper iridovirus (SGIV) stimulation, the mRNA expression of cathepsin B in E. coioides was up-regulated at 24 h post-infection. Subcellular localization analysis revealed that Ec-CB was distributed predominantly in the cytoplasm. When the fish cells (GS or FHM) were treated with the cathepsin B specific inhibitor CA-074Me, the occurrence of CPE induced by SGIV was delayed, and the viral gene transcription was significantly inhibited. Additionally, SGIV-induced typical apoptosis was also inhibited by CA-074Me in FHM cells. Taken together, our results demonstrated that the Ec-CB might play a functional role in SGIV infection.

  4. Spectral investigation of hot-spot and cavity resonance effects on the terahertz radiation emitted from high-Tc superconducting Bi2Sr2CaCu2O8+δ single crystal mesa structures

    Science.gov (United States)

    Kadowaki, Kazuo; Watanabe, Chiharu; Minami, Hidetoshi; Yamamoto, Takashi; Kashiwagi, Takanari; Klemm, Richard

    2014-03-01

    Terahertz (THz) electromagnetic radiation emitted from high-Tc superconducting Bi2Sr2CaCu2O8+δ mesa structures in the case of single mesa and series-connected mesas is investigated by the FTIR spectroscopic technique while observing its temperature distribution simultaneously by a SiC photoluminescence technique. Changing the bias level, sudden jumps of the hot-spot position were clearly observed. Although the radiation intensity changes drastically associated with the jump of the hot spot position, the frequency is unaffected as long as the voltage per junction is kept constant. Since the frequency of the intense radiation satisfies the cavity resonance condition, we confirmed that the cavity resonance is of primarily importance for the synchronization of whole intrinsic Josephson junctions in the mesa for high power radiation. This work was supported in part by the Grant-in-Aid for challenging Exploratory Research, the Ministry of Education, Culture, Sports, Science & Technology (MEXT).

  5. Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone.

    Science.gov (United States)

    Hegyi, Anna; Szabó, Mónika; Olasz, Ferenc; Kiss, János

    2017-09-06

    Dissemination of multiresistance has been accelerating among pathogenic bacteria in recent decades. The broad host-range conjugative plasmids of the IncA/C family are effective vehicles of resistance determinants in Gram-negative bacteria. Although more than 150 family members have been sequenced to date, their conjugation system and other functions encoded by the conserved plasmid backbone have been poorly characterized. The key cis-acting locus, the origin of transfer (oriT), has not yet been unambiguously identified. We present evidence that IncA/C plasmids have a single oriT locus immediately upstream of the mobI gene encoding an indispensable transfer factor. The fully active oriT spans ca. 150-bp AT-rich region overlapping the promoters of mobI and contains multiple inverted and direct repeats. Within this region, the core domain of oriT with reduced but detectable transfer activity was confined to a 70-bp segment containing two inverted repeats and one copy of a 14-bp direct repeat. In addition to oriT, a second locus consisting of a 14-bp imperfect inverted repeat was also identified, which mimicked the function of oriT but which was found to be a recombination site. Recombination between two identical copies of these sites is RecA-independent, requires a plasmid-encoded recombinase and resembles the functioning of dimer-resolution systems.

  6. Improved Detection of Candida sp. fks Hot Spot Mutants by Using the Method of the CLSI M27-A3 Document with the Addition of Bovine Serum Albumin▿†

    Science.gov (United States)

    Garcia-Effron, Guillermo; Park, Steven; Perlin, David S.

    2011-01-01

    Echinocandins are highly bound to serum proteins, altering their antifungal properties. The addition of 50% human serum to the MIC assay improves the identification of echinocandin-resistant Candida spp. harboring fks hot spot mutations. However, this modification cannot readily be applied to the method of the CLSI M27-A3 document due to safety and standardization difficulties. The aim of this study was to evaluate commercial bovine serum albumin (BSA) as a safe and standardized alternative to human serum. A collection of 28 echinocandin-susceptible strains, 10 Candida parapsilosis sensu lato strains (with naturally reduced echinocandin susceptibility), and 40 FKS hot spot mutants was used in this work. When RPMI 1640 was used for susceptibility testing, wild-type strains and fks mutants showed MIC range overlaps (−2, −1, and −3 2-fold-dilution steps separated these populations for anidulafungin, caspofungin, and micafungin, respectively). On the other hand, the addition of BSA to RPMI 1640 differentially increased echinocandin MIC values for these groups of strains, allowing better separation between populations, with no MIC range overlaps for any of the echinocandin drugs tested. Moreover, the use of RPMI-BSA reduced the number of fks hot spot mutant isolates for which MIC values were less than or equal to the upper limit for the wild type (very major errors) from 9, 2, and 7 with RPMI alone to 3, 0, and 3 for anidulafungin, caspofungin, and micafungin, respectively. When RPMI-BSA was used to study the susceptibility of C. parapsilosis sensu lato species to echinocandins, the strains behaved as anidulafungin- and micafungin-resistant isolates (MIC, ≥8 μg/ml). These data support the need for a revision of the CLSI protocol for in vitro testing of echinocandin susceptibility in order to identify all or most of the fks hot spot mutants. Also, caspofungin could be used as a surrogate marker of reduced susceptibility to echinocandins. PMID:21383097

  7. Global occurrence of archaeal amoA genes in terrestrial hot springs.

    Science.gov (United States)

    Zhang, Chuanlun L; Ye, Qi; Huang, Zhiyong; Li, Wenjun; Chen, Jinquan; Song, Zhaoqi; Zhao, Weidong; Bagwell, Christopher; Inskeep, William P; Ross, Christian; Gao, Lei; Wiegel, Juergen; Romanek, Christopher S; Shock, Everett L; Hedlund, Brian P

    2008-10-01

    Despite the ubiquity of ammonium in geothermal environments and the thermodynamic favorability of aerobic ammonia oxidation, thermophilic ammonia-oxidizing microorganisms belonging to the crenarchaeota kingdom have only recently been described. In this study, we analyzed microbial mats and surface sediments from 21 hot spring samples (pH 3.4 to 9.0; temperature, 41 to 86 degrees C) from the United States, China, and Russia and obtained 846 putative archaeal ammonia monooxygenase large-subunit (amoA) gene and transcript sequences, representing a total of 41 amoA operational taxonomic units (OTUs) at 2% identity. The amoA gene sequences were highly diverse, yet they clustered within two major clades of archaeal amoA sequences known from water columns, sediments, and soils: clusters A and B. Eighty-four percent (711/846) of the sequences belonged to cluster A, which is typically found in water columns and sediments, whereas 16% (135/846) belonged to cluster B, which is typically found in soils and sediments. Although a few amoA OTUs were present in several geothermal regions, most were specific to a single region. In addition, cluster A amoA genes formed geographic groups, while cluster B sequences did not group geographically. With the exception of only one hot spring, principal-component analysis and UPGMA (unweighted-pair group method using average linkages) based on the UniFrac metric derived from cluster A grouped the springs by location, regardless of temperature or bulk water pH, suggesting that geography may play a role in structuring communities of putative ammonia-oxidizing archaea (AOA). The amoA genes were distinct from those of low-temperature environments; in particular, pair-wise comparisons between hot spring amoA genes and those from sympatric soils showed less than 85% sequence identity, underscoring the distinctness of hot spring archaeal communities from those of the surrounding soil system. Reverse transcription-PCR showed that amoA genes were

  8. Impact of Hot and Cold Exposure on Human Skeletal Muscle Gene Expression

    Science.gov (United States)

    Zak, Roksana B.; Shute, Robert J.; Heesch, Matthew W.S.; La Salle, D. Taylor; Bubak, Matthew P.; Dinan, Nicholas E.; Laursen, Terence L.; Slivka, Dustin R.

    2017-01-01

    Many human diseases lead to a loss of skeletal muscle metabolic function and mass. Local and environmental temperature can modulate the exercise-stimulated response of several genes involved in mitochondrial biogenesis and skeletal muscle function in a human model. However, the impact of environmental temperature, independent of exercise, has not been addressed in a human model. Thus, the purpose of this study was to compare the effects of exposure to hot, cold, and room temperature conditions on skeletal muscle gene expression related to mitochondrial biogenesis and muscle mass. METHODS Recreationally trained male subjects (n=12) had muscle biopsies taken from the vastus lateralis before and after 3 h exposure to hot (33 °C), cold (7 °C), or room temperature (20 °C) conditions. RESULTS Temperature had no effect on most of the genes related to mitochondrial biogenesis, myogenesis, or proteolysis (p > 0.05). Core temperature was significantly higher in hot and cold environments compared to room temperature (37.2 ± 0.1 °C, p = 0.001; 37.1 ± 0.1 °C, p = 0.013; 36.9 ± 0.1 °C, respectively). Whole body oxygen consumption was also significantly higher in hot and cold compared to room temperature (0.38 ± 0.01 L·min−1, p < 0.001; 0.52 ± 0.03 L·min−1, p < 0.001; 0.35 ± 0.01 L·min−1, respectively). CONCLUSIONS These data show that acute temperature exposure alone does not elicit significant changes in skeletal muscle gene expression. When considered in conjunction with previous research, exercise appears to be a necessary component to observe gene expression alterations between different environmental temperatures in humans. PMID:28177744

  9. New insights into the roles of host gene-necrotrophic effector interactions in governing susceptibility of durum wheat to tan spot and Septoria nodorum blotch

    Science.gov (United States)

    Tan spot and Septoria nodorum blotch (SNB) are important diseases of wheat caused by the necrotrophic fungi Pyrenophora tritici-repentis and Parastagonospora nodorum, respectively. The P. tritici-repentis necrotrophic effector (NE) Ptr ToxB causes tan spot when recognized by the Tsc2 gene. The NE To...

  10. Hot-spot analysis applied to the identification of potential high and low regulating, providing and cultural ecosystem services in Vilnius Region (Lithuania)

    Science.gov (United States)

    Pereira, Paulo; Daniel, Depellegrin; Egarter-Vigl, Lukas; Cerda, Artemi; Estebaranz, Ferran; Misiune, Ieva

    2017-04-01

    Ecosystem services (ES) potential assessment is crucial for a correct territorial planning at different scales of analysis (Depellegrin et al., 2016). In urban and peri-urban areas, sprawl, grazing and unsustainable agriculture practices contributed to land degradation and de decrease of the quality and quantity of the services provided by these areas (Eldridge and Delgado-Baquerizo, 2017; Favretto et al., 2017). In order to understand the spatial pattern of these impacts, mapping ES potential is key to understand the areas that need to be restored and protected for an unsustainable use (Brevik et al., 2016; Egarter-Vigl et al., 2017; Pereira et al., 2017). Hot-spot analysis is a good method to identify clusters of areas with high and low capacity for ES capacity. This analysis is very useful to detect homogeneous areas, where ES have high or low quality. The objective of this work is to apply a hot-spot analysis to detect areas with high/low capacity for Regulating, Provision, Cultural and Total ES in Vilnius region. ES potential was carried out based on the matrix developed by Burkhard et al. (2009), which ranks ES capacity from 0= no capacity to 5=very high relevant capacity to a different land use type. The results showed that regulating, providing and cultural and total ES have a significant dispersed (low-clustered) pattern: Regulating (Z-score=-19.28, purban sprawl observed in Vilnius area (Pereira et al., 2014). References Brevik, E., Calzolari, C., Miller, B., Pereira, P., Kabala, C., Baumgarten, A., Jordán, A. (2016) Historical perspectives and future needs in soil mapping, classification and pedological modelling, Geoderma, 264, Part B, 256-274. Burkhard B, Kroll F, Müller F, Windhorst W. 2009. Landscapes' capacities to provide ecosystem services- a concept for land-cover based assessments. Landscape Online. 15, 1-22. Depellegrin, D.A., Pereira, P., Misiune, I., Egarter-Vigl, L. Mapping Ecosystem Services potential in Lithuania. International Journal

  11. High-resolution measurement of nitrous oxide in the Elbe estuary under hypoxia: Hot-spots of biological N2O production

    Science.gov (United States)

    Brase, Lisa; Lendt, Ralf; Sanders, Tina; Dähnke, Kirstin

    2016-04-01

    Nitrous oxide (N2O) is one of the most important greenhouse gases. Its global warming potential exceeds that of CO2 by a factor of ˜300. Estuaries, being sites of intense biological N-turnover, are one of the major natural sources of N2O emissions. On two ship cruises in April and June 2015, concentrations of N2O were measured in the surface water using equilibrator laser based on-line measurements. Based on these high-resolution N2O profiles along the Elbe estuary, N2O saturation and N2O-fluxes between surface water and air were calculated. Additionally, DIN concentrations and dual stable isotopes of nitrate (δ15N and δ18O) were analyzed. Concentration and water-to-air fluxes of N2O were highest in the Hamburg port region and dropped quickly further downstream. Highest water-to-air fluxes were up to 800μM/m2/d and 1600μM/m2/d in April and in June, respectively. Downstream of the port region, an N2O oversaturation of 150-200% was estimated over the entire estuary, with saturation approaching equilibrium (96-100%) only in the North Sea region. N2O production was much higher in June than in April 2015, likely coupled to lower oxygen saturation in the water column in June. Based on these measurements, the port of Hamburg region was identified as a hot-spot of N2O production. High N2O concentration and depleted values of nitrate isotopes suggest that nitrification is a significant source of N2O in the estuary, especially at low oxygen concentration. In the Elbe estuary, hypoxia obviously drastically increased the emissions of the greenhouse gas N2O.

  12. DnaK as Antibiotic Target: Hot Spot Residues Analysis for Differential Inhibition of the Bacterial Protein in Comparison with the Human HSP70.

    Directory of Open Access Journals (Sweden)

    Federica Chiappori

    Full Text Available DnaK, the bacterial homolog of human Hsp70, plays an important role in pathogens survival under stress conditions, like antibiotic therapies. This chaperone sequesters protein aggregates accumulated in bacteria during antibiotic treatment reducing the effect of the cure. Although different classes of DnaK inhibitors have been already designed, they present low specificity. DnaK is highly conserved in prokaryotes (identity 50-70%, which encourages the development of a unique inhibitor for many different bacterial strains. We used the DnaK of Acinetobacter baumannii as representative for our analysis, since it is one of the most important opportunistic human pathogens, exhibits a significant drug resistance and it has the ability to survive in hospital environments. The E.coli DnaK was also included in the analysis as reference structure due to its wide diffusion. Unfortunately, bacterial DnaK and human Hsp70 have an elevated sequence similarity. Therefore, we performed a differential analysis of DnaK and Hsp70 residues to identify hot spots in bacterial proteins that are not present in the human homolog, with the aim of characterizing the key pharmacological features necessary to design selective inhibitors for DnaK. Different conformations of DnaK and Hsp70 bound to known inhibitor-peptides for DnaK, and ineffective for Hsp70, have been analysed by molecular dynamics simulations to identify residues displaying stable and selective interactions with these peptides. Results achieved in this work show that there are some residues that can be used to build selective inhibitors for DnaK, which should be ineffective for the human Hsp70.

  13. DNA barcoding in a biodiversity hot spot: potential value for the identification of Malagasy Euphorbia L. listed in CITES Appendices I and II.

    Science.gov (United States)

    Aubriot, Xavier; Lowry, Porter P; Cruaud, Corinne; Couloux, Arnaud; Haevermans, Thomas

    2013-01-01

    The island of Madagascar is a key hot spot for the genus Euphorbia, with at least 170 native species, almost all endemic. Threatened by habitat loss and illegal collection of wild plants, nearly all Malagasy Euphorbia are listed in CITES Appendices I and II. The absence of a reliable taxonomic revision makes it particularly difficult to identify these plants, even when fertile, and thereby compromises the application of CITES regulations. DNA barcoding, which can facilitate species-level identification irrespective of developmental stage and the presence of flowers or fruits, may be a promising tool for monitoring and controlling trade involving threatened species. In this study, we test the potential value of barcoding on 41 Euphorbia species representative of the genus in Madagascar, using the two widely adopted core barcode markers (matK and rbcL), along with two additional DNA regions, nuclear internal transcribed spacer (ITS) and the chloroplastic intergenic spacer psbA-trnH. For each marker and for selected marker combinations, inter- and intraspecific distance estimates and species discrimination rates are calculated. Results using just the 'official' barcoding markers yield overlapping inter- and intraspecific ranges and species discrimination rates below 60%. When ITS is used, whether alone or in combination with the core markers, species discrimination increases to nearly 100%, whereas the addition of psbA-trnH produces less satisfactory results. This study, the first ever to test barcoding on the large, commercially important genus Euphorbia shows that this method could be developed into a powerful identification tool and thereby contribute to more effective application of CITES regulations.

  14. Structure of the lithosphere-asthenosphere system in the vicinity of the Tristan da Cunha hot spot as seen by surface waves

    Science.gov (United States)

    Geissler, Wolfram; Bonadio, Raffaele; Lebedev, Sergei; Jokat, Wilfried; Jegen, Marion; Baba, Kiyoshi; Sens-Schönfelder, Christoph

    2016-04-01

    According to classical plume theory, the Tristan da Cunha hotspot is thought to have played a major role in the rifting of the South Atlantic margins and the creation of the aseismic Walvis Ridge by impinging at the base of the continental lithosphere shortly before or during the breakup of the South Atlantic margins. However, Tristan da Cunha is enigmatic as it cannot be clearly identified as a hot spot but may also be classified as a more shallow type of anomaly that may actually have been caused by the opening of the South Atlantic. The equivocal character of Tristan da Cunha is largely due to a lack of geophysical and petrological data in this region. We therefore staged a multi-disciplinary geophysical study of the region by acquiring passive marine electromagnetic and seismic data, and bathymetric data within the framework of the SPP1375 South Atlantic Margin Processes and Links with onshore Evolution (SAMPLE) funded by the German Science foundation. The experiment included two ship expeditions onboard the German R/V MARIA S. MERIAN in 2012 and 2013. In our contribution we will present first results on the shear wave velocity structure of the lithosphere-asthenosphere system. We applied the classical two-station method; Rayleigh wave dispersion curves are determined by cross-correlation of seismograms from a pair of station. We measured interstation phase velocities of (earthquake-excited) fundamental-mode surface waves in a period range of 10 to 60 s. The selection of acceptable phase-velocity measurements in the frequency domain had to be done manually for each event. We present phase-velocity maps for the study area. Furthermore, we present 1D shear wave velocity models inverted from the highest-quality observations.

  15. Hunting for right and left parietal hot spots using single-pulse TMS: modulation of visuospatial perception during line bisection judgment in the healthy brain.

    Science.gov (United States)

    Salatino, Adriana; Poncini, Marisa; George, Mark S; Ricci, Raffaella

    2014-01-01

    A series of studies have consistently reproduced left neglect-like bias on line length estimation tasks in healthy participants by applying transcranial magnetic stimulation (TMS) over the right posterior parietal cortex (PPC), while no significant changes have been reported when stimulating the left PPC. However, a notable inter-individual variability in the right parietal site where TMS modulates visuospatial perception can be observed, and no general agreement exists on how to identify the optimal parietal site of stimulation. In the present study, we propose a new site-finding TMS protocol to easily identify the optimum parietal location, or "hot spot," where TMS may modulate visuospatial perception on a line length estimation task (the Landmark task). Single-pulse TMS at 115% of motor threshold was applied 150 ms after the visual stimulus onset over nine different sites of a 3 cm × 3 cm grid, centred over right or left PPC (P4 and P3 according to the 10-20 EEG system, respectively) in eight healthy participants. Stimulation of right PPC induced a significant left neglect-like bias, when the coil was applied over the most posterior and dorso-posterior sites. Unexpectedly, TMS over left PPC also produced left neglect-like bias. However, in this case significant effects were found when targeting the most anterior and dorso-anterior portions of the grid. These results are discussed in relation to recent findings on neural networks underlying spatial cognition. The hunting protocol we propose might offer an economical and easy-to-use tool to functionally identify the optimal parietal site where TMS can modulate visuospatial perception, in healthy subjects and possibly in post-stroke patients undergoing repetitive transcranial magnetic stimulation treatment.

  16. 从文献分析看突发性耳聋的研究热点%Analysis the Hot Spots of Sudden Deafness by Researching Articles

    Institute of Scientific and Technical Information of China (English)

    向杰; 刘博

    2015-01-01

    Objective To better understand the hot spots and breakthrough on sudden deafness by searching and reading the papers in recent 5 years published in the PUBMED and the CNKI.Methods Use the Mesh term“Hearing Loss,Sudden”to search in the PUBMED.Use“sudden deafness”for the theme word to search in the CNKI.Results There are 381 articles in English,179 articles in Chinese.The article number of both database increase annually and the numbers are more than other years’in the 2012.(1)The top 3 countries published articles about sudden deafness in English are England,China,and Germany. The top 3 cities published articles about sudden deafness in Chinese are Guangdong,Henan,and Beijing.(2)There are 72(18.9%) articles about etiology of sudden deafness in English,61(16.0%) articles about diagnosis of sudden deafness in English,61(16.0%) articles about drug therapy.Articles about etiology and diagnosis in Chinese are less than in English.They focused in the therapy of sudden deafness. There are 61(34.1%) articles about drug therapy in Chinese,40(22.3%) articles about other therapy in Chinese,13(7.3%) about laboratory examination in Chinese.ConclusionThe hot spots of sudden deafness is significant different between home and abroad.The hot spots of sudden deafness is etiology,pathogenesis,and treatment.%目的:通过对PUBMED和CNKI数据库近5年收录的突聋研究文献检索、阅读和分析,了解近期突聋研究的热点问题和研究进展。方法在PUBMED数据库高级检索Mesh词表字段中检索“Hearing Loss,Sudden”,统计文献检索结果;在CNKI数据库主题字段中检索“突聋”,统计文献检索结果。结果 PUBMED及CNKI中近5年突聋文献发表数量呈逐年增加状态。共检索到英文文献381篇,中文文献179篇。①按地区分布看,英文文献数量前3位分别是英格兰、中国和德国。中文文献数量前3位分别是广东、河南和北京。②按文献研究方向看,英文文

  17. Gene expression profiling in gill tissues of White spot syndrome virus infected black tiger shrimp Penaeus monodon by DNA microarray.

    Science.gov (United States)

    Shekhar, M S; Gomathi, A; Gopikrishna, G; Ponniah, A G

    2015-06-01

    White spot syndrome virus (WSSV) continues to be the most devastating viral pathogen infecting penaeid shrimp the world over. The genome of WSSV has been deciphered and characterized from three geographical isolates and significant progress has been made in developing various molecular diagnostic methods to detect the virus. However, the information on host immune gene response to WSSV pathogenesis is limited. Microarray analysis was carried out as an approach to analyse the gene expression in black tiger shrimp Penaeus monodon in response to WSSV infection. Gill tissues collected from the WSSV infected shrimp at 6, 24, 48 h and moribund stage were analysed for differential gene expression. Shrimp cDNAs of 40,059 unique sequences were considered for designing the microarray chip. The Cy3-labeled cRNA derived from healthy and WSSV-infected shrimp was subjected to hybridization with all the DNA spots in the microarray which revealed 8,633 and 11,147 as up- and down-regulated genes respectively at different time intervals post infection. The altered expression of these numerous genes represented diverse functions such as immune response, osmoregulation, apoptosis, nucleic acid binding, energy and metabolism, signal transduction, stress response and molting. The changes in gene expression profiles observed by microarray analysis provides molecular insights and framework of genes which are up- and down-regulated at different time intervals during WSSV infection in shrimp. The microarray data was validated by Real Time analysis of four differentially expressed genes involved in apoptosis (translationally controlled tumor protein, inhibitor of apoptosis protein, ubiquitin conjugated enzyme E2 and caspase) for gene expression levels. The role of apoptosis related genes in WSSV infected shrimp is discussed herein.

  18. Equivalent hot spot stress approach for multiaxial fatigue strength assessment of complex welded joints%复杂焊接接头多轴疲劳强度评估的等效热点应力法

    Institute of Scientific and Technical Information of China (English)

    刘刚; 黄如旭; 黄一

    2012-01-01

    The current hot spot stress approach still has some limitations to the multiaxial fatigue strength assessment of complex welded joints.In this paper,an new stress estimation approach for the multiaxial fatigue strength assessment of complex welded joints is proposed,in which an equivalent hot spot stress is defined at a point(called Zero Point) in plate thickness direction where nonlinear distribution stress due to the notch effect of the weld is equal to zero.The proposed approach is based on that the nonlinear stress peak is not included in the hot spot stress and the nonlinear distribution stress is in self-equilibrium.First,influence factors and change laws of the Zero Point position were discussed,and a fitting equation for determining the Zero Point position was established.Then,published fatigue tests data and numerical stress results of some welded joints were used for comparison between the proposed equivalent hot spot stress approach and the current hot spot stress approach.The equivalent hot spot stress approach was verified to be consistent with the current hot spot stress approach and also have higher accuracy for considering the thickness effect on the fatigue strength to a certain extent.The merit of the proposed approach is that it can be used to estimate the multiaxial fatigue strength of welded joints through combining multiaxial fatigue theory.%基于焊缝本身引起的非线性分布应力自平衡的性质,在板厚方向非线性分布应力大小为零的位置(零点位置)定义等效热点应力,提出一种新的能够用于复杂焊接接头多轴疲劳应力评价方法.首先,分析了零点位置的影响因素和变化规律,建立了确定零点位置的拟合方程.然后,和公开发表的试验数据以及现有的热点应力法有限元计算结果进行了对比.结果表明,等效热点应力法和现有的热点应力法具有很好的一致性,而且能够在一定程度上考虑厚度效应对疲劳强度的影

  19. Polymorphisms of Leptin-b Gene Associated with Growth Traits in Orange-Spotted Grouper (Epinephelus coioides

    Directory of Open Access Journals (Sweden)

    Hai Huang

    2014-07-01

    Full Text Available In mammals, leptin has been demonstrated to perform important roles in many physiological activities and to influence development, growth, metabolism and reproduction. However, in fish, its function is still unclear. Duplicate leptin genes, leptin-a and leptin-b, have been identified in the orange-spotted grouper. In the present study, the polymorphisms in the leptin-b gene of the orange-spotted grouper were detected, and the relation between these polymorphisms and 12 growth traits were analyzed. Six polymorphisms (including 3 single nucleotide polymorphisms (c.14G>A, c.93A>G, c.149G>A in exon 1, 2 SNPs (c.181A>G, c.193G>A in intron 1, and 1 SNP (c.360C>T in exon 2 were identified and genotyped from 200 different individuals. The results revealed that the SNP c.149G>A was significantly associated with growth traits, that the heterozygous mutation genotype GA having negative effects on growth traits. However, the other five SNPs (c.14G>A, c.93A>G, c.181A>G, c.193G>A, c.360C>T did not show significant associations with all the growth traits. Compared with our findings in leptin-a gene, the results suggested that the leptin-a hormone has more important physiological effects in fish bodies than the leptin-b type. Moreover, leptin genes were supposed to be one class of major candidate genes of regulating growth traits in the orange-spotted grouper.

  20. Polymorphisms of leptin-b gene associated with growth traits in orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Huang, Hai; Wei, Yun; Meng, Zining; Zhang, Yong; Liu, Xiaochun; Guo, Liang; Luo, Jian; Chen, Guohua; Lin, Haoran

    2014-07-07

    In mammals, leptin has been demonstrated to perform important roles in many physiological activities and to influence development, growth, metabolism and reproduction. However, in fish, its function is still unclear. Duplicate leptin genes, leptin-a and leptin-b, have been identified in the orange-spotted grouper. In the present study, the polymorphisms in the leptin-b gene of the orange-spotted grouper were detected, and the relation between these polymorphisms and 12 growth traits were analyzed. Six polymorphisms (including 3 single nucleotide polymorphisms (c.14G>A, c.93A>G, c.149G>A) in exon 1, 2 SNPs (c.181A>G, c.193G>A) in intron 1, and 1 SNP (c.360C>T) in exon 2) were identified and genotyped from 200 different individuals. The results revealed that the SNP c.149G>A was significantly associated with growth traits, that the heterozygous mutation genotype GA having negative effects on growth traits. However, the other five SNPs (c.14G>A, c.93A>G, c.181A>G, c.193G>A, c.360C>T) did not show significant associations with all the growth traits. Compared with our findings in leptin-a gene, the results suggested that the leptin-a hormone has more important physiological effects in fish bodies than the leptin-b type. Moreover, leptin genes were supposed to be one class of major candidate genes of regulating growth traits in the orange-spotted grouper.

  1. Expression profiles of hot pepper (Capsicum annuum) genes under cold stress conditions

    Indian Academy of Sciences (India)

    Eul-Won Hwang; Kyung-A Kim; Soo-Chul Park; Mi-Jeong Jeong; Myung-Ok Byun; Hawk-Bin Kwon

    2005-12-01

    In an attempt to determine a cold defense mechanism in plants, we have attempted to characterize changes occurring in the expression of cold-regulated transcript levels in the hot pepper (Capsicum annuum), using cDNA microarray analysis, combined with Northern blot analysis. After analysing a 3.1 K hot pepper cDNA microarray, we isolated a total of 317 cold inducible genes. We selected 42 genes which were up-regulated and three genes which were down-regulated due to cold treatment, for further analysis. Among the 45 genes which appeared to be up-regulated by cold, 19 genes appeared to be simultaneously regulated by salt stress. Among the up-regulated cold-stress genes, we identified a variety of transcription factors, including: a family of 4 ethylene-responsive element binding protein (EREBP, designated CaEREBP-C1 to C4) genes, a bZIP protein (CaBZ1), RVA1, Ring domain protein, HSF1, and the WRKY (CaWRKY1) protein. As mentioned earlier, several genes appeared to be induced not only by cold stress, but also simultaneously by salt stress. These genes included: CaEREBP-C3, CaBZ1, putative trans-activator factor, NtPRp27, malate dehydrogenase, putative auxin-repressed protein, protein phosphatase (CaTPP1), SAR8.2 protein precursor, late-embryogenesis abundant protein 5 (LEA5), DNAJ protein homologue, xyloglucanendo-1,4--D-gucanase precursor, PR10, and the putative non-specific lipid transfer protein StnsLTP.

  2. Cloning and expression of dnaK gene from Bacillus pumilus of hot water spring origin

    Directory of Open Access Journals (Sweden)

    Murugan Kumar

    2014-03-01

    Full Text Available A set of thermotolerant strains isolated from hot springs of Manikaran and Bakreshwar (India were selected with an aim to isolate dnak gene which encodes DnaK protein. The gene dnaK along with its flanking region was successfully amplified from 5 different strains (4 from Bakreshwar and one from Manikaran. Restriction fragment length polymorphism (RFLP revealed that amplicons were almost identical in sequence. The dnak gene from one representative, Bacillus pumilus strain B3 isolated from Bakreshwar hot springs was successfully cloned and sequenced. The dnaK gene was flanked by gene grpE on one side. The dnaK gene was 1842 bp in length encoding a polypeptide of 613 amino acid residues. Calculated molecular weight and pI of the protein were 66,128.36 Da and 4.72 respectively. The deduced amino acid sequence of this gene shared high sequence homology with other DnaK proteins and its homologue Hsp 70 from other microorganisms, but possessed 36 substitutions and two insertions, as compared to DnaK protein of Bacillus subtilis. The dnaK gene of B. pumilus was successfully expressed in Escherichia coli BL 21 (DE3 using pET expression systems. Heterologous expression of dnaK of B. pumilus in E. coli BL 21 (DE3 allowed for the growth of E. coli up to 50 °C and survival up to 60 °C for 16 h, suggesting that dnak from B. pumilus imparts tolerance to host cells under high temperature. This novel gene can be an important component for possible utilization in abiotic stress management of plants.

  3. Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Two fragments SCS3620 and SCS3580 of the co-dominant marker OPS03620&580 that were linked to the resistance gene of soybean frog-eye leaf spot have been completely sequenced.A significant insertion of 30 bp is the main reason of the polymorphism between the two fragments.The results of Southern hybridization indicate that SCS3620 derives from a single- or low-copy sequence and can be used as an RFLP probe.A co-dominant SCAR marker SCS3620&580 has been developed based on the sequences.The segregation of SCS3620&580 is similar to that of RAPD marker OPS03620&580.Significant polymorphism has been shown between resistant and susceptible genotypes when 62 soybean genotypes were surveyed for the SCAR marker.Therefore,the marker can be used in the resistance breeding of soybean frog-eye leaf spot by marker-assisted selection.

  4. 利用卫星热点测报森林火灾的报准率统计分析%Accuracy Statistical Analysis of Forest Fire Forecasting by Using Satellite Hot Spot

    Institute of Scientific and Technical Information of China (English)

    黄甫则; 周汝良; 叶江霞; 丁琨

    2012-01-01

    卫星热点测报森林火灾的本质是测报地面高温热源,粗分辨率的遥感数据不能有效区别森林火灾与其非森林火灾,实地核查是卫星热点测报中的一个工作环节,利用GIS系统制定计算机辅助决策,提升热点处置的技术水平,是森林防火行业提出的一个应用研究课题,评估卫星热点测报森林火灾的报准率是解决问题的基础.通过收集云南省1998~2002年和2004年的卫星热点监测反馈数据11251条,按8种热源进行分类统计,进行大样本统计分析,利用总体比例估计模型进行比估计.结果表明,在90%可靠性条件下,云南的卫星热点中,森林火灾所占的比估计为29.3%,置信区间为{0.286,0.3001}.%The nature of satellite hot forest fires forecasting is measuring high-temperature heat source on the ground. Coarse-resolution remote sensing data can not distinguish between forest fires and non-forest fires. Field verification is a working link in satellite hot spot forecast. Using GIS system to develop computer-aided decision-making to enhance technical level of hot spot analysis is an applied research topic brought up by forest fire prevention profession. The assessment of accuracy of forest fires satellite hot spot forecasting is the basis of problem solving. This article collected 11 251 Satellite hot spot monitoring feedback data in Yunnan Province from 1998 to 2002 and in 2004. Classification statistics was made with eight kinds of heat source, and statistical analysis of large sample was made, the overall proportion estimated model was used for proportion estimation. The results showed that under 90% reliability condition, satellite hotspots in Yunnan, the share of forest fires proportion estimation was of 29. 3% , the confidence interval was 10. 286, 0. 300 11.

  5. Converter Compressor Building, SWMU 089, Hot Spot Areas 1, 2, and 5 Operations, Maintenance, and Monitoring Report, Kennedy Space Center, Florida

    Science.gov (United States)

    Wilson, Deborah M.

    2015-01-01

    This Operations, Maintenance, and Monitoring Report (OMMR) presents the findings, observations, and results from operation of the air sparging (AS) interim measure (IM) for Hot Spot (HS) Areas 1, 2, and 5 at the Converter Compressor Building (CCB) located at Kennedy Space Center (KSC), Florida. The objective of the IM at CCB HS Areas 1, 2, and 5 is to decrease concentrations of volatile organic compounds (VOCs) in groundwater in the treatment zones via AS to levels that will enable a transition to a monitored natural attenuation (MNA) phase. This OMMR presents system operations and maintenance (O&M) information and performance monitoring results since full-scale O&M began in June 2014 (2 months after initial system startup in April 2014), including quarterly performance monitoring events in July and October 2014 and January and May 2015. Based on the results to date, the AS system is operating as designed and is meeting the performance criteria and IM objective. The performance monitoring network is adequately constructed for assessment of IM performance at CCB HS Areas 1, 2, and 5. At the March 2014 KSC Remediation Team (KSCRT) Meeting, team consensus was reached for the design prepared for expansion of the system to treat the HS 4 area, and at the November 2014 KSCRT Meeting, team consensus was reached that HS 3 was adequately delineated horizontally and vertically and for selection of AS for the remedial approach for HS 3. At the July 2015 KSCRT meeting, team consensus was reached to continue IM operations in all zones until HSs 3 and 4 is operational, once HS 3 and 4 zones are operational discontinue operations in HS 1, 2, and 5 zones where concentrations are less than GCTLs to observe whether rebounding conditions occur. Team consensus was also reached to continue quarterly performance monitoring to determine whether operational zones achieve GCTLs and to continue annual IGWM of CCB-MW0012, CCBMW0013, and CCB-MW0056, located south of the treatment area. The

  6. Four new WMO/GAW Observatories for the investigation of trace gas and aerosol variability in the Mediterranean hot-spot

    Science.gov (United States)

    Cristofanelli, Paolo; Marinoni, Angela; Duchi, Rocco; Busetto, Maurizio; Calzolari, Francescopiero; Bourcier, Laureline; Landi, Tony Christian; Calidonna, Claudia; Contini, Daniele; Ammoscato, Ivano; Gulli', Daniel; Dinoi, Adelaide; Sprovieri, Francesca; Carbone, Francesco; Naccarato, Attilio; Mannarino, Valentino; Pirrone, Nicola; Bonasoni, Paolo

    2016-04-01

    The Mediterranean Basin is considered a hot-spot region in term of air-quality and climate change due to the impact of anthropogenic and natural processes. Nevertheless, permanent infrastructures for the observations and the recognition of the atmospheric composition variability changes are still too sparse in this region. To contribute to a more accurate and wide-spread observation system, in the framework of the Project "I-AMICA" - Advanced Infrastructure for the Environmental-Climatic Monitoring (a three years Italian National Operative Program - PON, co-founded by the European Regional Development Fund), four permanent Climatic-Environmental Observatories have been set up in the southern Italy: Lecce (Apulia Region, 40° 20' 8" N, 18° 07' 28" E, 37 m a.s.l.), Lamezia Terme (Calabria Region, 38° 52' 34" N, 16° 13' 56" E, 6 m a.s.l.), Capo Granitola (Sicily, 37° 34' N, 12° 39' E, 5 m a.s.l.) and Mt. Curcio (Calabria, 39° 31' N, 16° 42' E, 1796 m a.s.l). These Observatories were equipped with homogeneous and standardized experimental set-up for measurements of aerosol properties (number size distribution, absorption and scattering coefficient, mass, equivalent black carbon), reactive and greenhouse gases (O3, NO, NO2, SO2, CO, CO2, CH4). The I-AMICA Observatories represent different conditions of the Mediterranean region, from suburban conditions at Lecce to costal background conditions at Lamezia Terme and Capo Granitola and high-mountain remote conditions at Mt. Curcio. The integration of the information from this high-quality observation network can be used for studies of aerosol transport from marine environment and Sahara desert, as well as for investigation of secondary pollutants formation in the gaseous and aerosol phase, investigation of continental outflow to Mediterranean Sea, impacts of vessel emissions on regional air quality and trans-boundary pollution. In this work, we provide a preliminary overview of gas and aerosol variability, together

  7. Developing an automatic classification system of vegetation anomalies for early warning with the ASAP (Anomaly hot Spots of Agricultural Production) system

    Science.gov (United States)

    Meroni, M.; Rembold, F.; Urbano, F.; Lemoine, G.

    2016-12-01

    Anomaly maps and time profiles of remote sensing derived indicators relevant to monitor crop and vegetation stress can be accessed online thanks to a rapidly growing number of web based portals. However, timely and systematic global analysis and coherent interpretation of such information, as it is needed for example for SDG 2 related monitoring, remains challenging. With the ASAP system (Anomaly hot Spots of Agricultural Production) we propose a two-step analysis to provide monthly warning of production deficits in water-limited agriculture worldwide. The first step is fully automated and aims at classifying each administrative unit (1st sub-national level) into a number of possible warning levels, ranging from "none" to "watch" and up to "extended alarm". The second step involves the verification of the automatic warnings and integration into a short national level analysis by agricultural analysts. In this paper we describe the methodological development of the automatic vegetation anomaly classification system. Warnings are triggered only during the crop growing season, defined by a remote sensing based phenology. The classification takes into consideration the fraction of the agricultural and rangelands area for each administrative unit that is affected by a severe anomaly of two rainfall-based indicators (the Standardized Precipitation Index (SPI), computed at 1 and 3-month scale) and one biophysical indicator (the cumulative NDVI from the start of the growing season). The severity of the warning thus depends on the timing, the nature and the number of indicators for which an anomaly is detected. The prototype system is using global NDVI images of the METOP sensor, while a second version is being developed based on 1km Modis NDVI with temporal smoothing and near real time filtering. Also a specific water balance model is under development to include agriculture water stress information in addition to the SPI. The monthly warning classification and crop

  8. Field and laboratory studies of the nitrogen and oxygen isotopic composition of N2O: Corona discharge production, biomass burning, and ocean and "Arctic hot spot" emissions

    Science.gov (United States)

    Boering, K. A.

    2016-12-01

    While inverse modeling studies of atmospheric nitrous oxide (N2O) concentrations have narrowed uncertainties in the magnitudes, geographic distribution, and timing of N2O fluxes to the atmosphere that are needed to understand and to mitigate the rising concentration of this greenhouse gas and ozone depleting substance in the atmosphere, significant uncertainties remain, including accounting for the return of N2O-depleted air from the stratosphere. Measurements of the average and site-specific nitrogen and the oxygen isotopic compositions of N2O can provide an additional means to attribute observed N2O variations to its various sources or stratospheric sink [e.g., Park et al., 2012]. In this presentation, we will highlight recent laboratory work determining the isotopic composition of N2O produced in a corona discharge (the process that produces N2O in thunderstorms), showing it has an isotopic fingerprint that is distinct from that for soil and ocean emissions, for biomass burning, and for the return of air from the stratosphere. Although N2O produced by lightning is only a small fraction of the global annual source of N2O to the atmosphere, the large and unique isotopic signature of corona discharge N2O now characterized completes the array needed to identify the origin, for example, of the unexplained N2O enhancements measured in the tropical and subtropical upper troposphere during the 2009 HIPPO mission [Wofsy 2011]. Such N2O enhancements may also be consistent with inverse modeling studies [e.g., Hirsch et al., 2006; Huang et al., 2008] suggesting tropical N2O source(s) must be larger than expected from bottom-up inventories, so identifying the source of these enhancements is critical. Isotope compositions of N2O in a biomass burning plume in the tropical upper troposphere, from a Southern Ocean ship cruise, and from an Arctic peat circle `hot spot' will also be briefly compared and contrasted with the corona discharge results. Hirsch, A.I., et al., Glob

  9. Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp

    Science.gov (United States)

    Ren, Qian; Huang, Xin; Cui, Yalei; Sun, Jiejie; Wang, Wen

    2017-01-01

    ABSTRACT In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. An miRNA can generally target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spaztle (Spz)-Toll-Dorsal-antilipopolysaccharide factor (ALF) signaling pathway plays a very important role in antiviral immunity against invasion of white spot syndrome virus (WSSV) in shrimp (Marsupenaeus japonicus). Dorsal, the central gene in the Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection. The regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study contributes novel insights into the viral miRNA-mediated Toll signaling pathway during the virus-host interaction. IMPORTANCE An miRNA can target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs during virus infection has not yet been extensively explored. The results of this study indicated that the shrimp Dorsal gene, the central gene in the Toll pathway, was targeted by two viral miRNAs during infection with white spot syndrome virus. Regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study provides new insight into the viral miRNA-mediated Toll signaling pathway in virus-host interactions. PMID:28179524

  10. Immunoglobulin genes and antibody responses in the spotted wolffish (Anarhichas minor Olafsen).

    Science.gov (United States)

    Espelid, S; Halse, M; Solem, S T; Jørgensen, T O

    2001-07-01

    The spotted wolffish Anarhichas minor Olafsen is a promising new species in aquaculture in the cold waters of northern Norway. In this paper, some basic immunological studies of this marine species are reported. Of comparative interest are the cDNA sequences of the immunoglobulin transcript and the antibody responses to model antigens. Of more practical importance are the humoral immune responses and antibody specificities to potentially pathogenic bacteria. Full length cDNA clones encoding the immunoglobulin heavy and light chains in the spotted wolffish were sequenced demonstrating variable degrees of similarity to other teleost fish species. Also in the spotted wolffish the CH4 domain was deleted in the transmembrane form of the immunoglobulin heavy chain (IgH) as a receptor on B cells, with the transmembrane exon spliced directly to the CH3 domain. The antibody responses to various antigens like hapten-carrier molecules, protein antigens and bacterial pathogens were relatively high, but with some interesting exceptions. Anti-hapten responses to NIP and FITC were high while anti-DNS responses were low, but more surprisingly, there was hardly any B-cell response to the carrier molecule LPH. On the other hand, protein antigens like CGG and BSA were highly immunogenic in the spotted wolffish as were the bacterial antigens Vibrio anguillarum, V. salmonicida and Aeromonas salmonicida.

  11. Transcriptional analysis of the ribonucleotide reductase genes in shrimp white spot syndrome virus

    NARCIS (Netherlands)

    Tsai, M.F.; Lo, C.F.; Hulten, van M.C.W.; Tzeng, H.F.; Chou, C.M.; Huang, C.J.; Wang, C.S.

    2000-01-01

    The causative agent of white spot syndrome (WSS) is a large double-stranded DNA virus, WSSV, which is probably a representative of a new genus, provisionally called Whispovirus. From previously constructed WSSV genomic libraries of a Taiwan WSSV isolate, clones with open reading frames (ORFs) that

  12. Three functionally diverged major White Spot Syndrome Virus structural proteins evolved by gene duplication

    NARCIS (Netherlands)

    Hulten, van M.C.W.; Goldbach, R.W.; Vlak, J.M.

    2000-01-01

    White spot syndrome virus (WSSV) is an invertebrate virus causing considerable mortality in penaeid shrimp. The oval-to-bacilliform shaped virions, isolated from infected Penaeus monodon, contain four major proteins: VP28, VP26, VP24 and VP19 (28, 26, 24 and 19 kDa, respectively). VP26 and VP24 are

  13. Transcriptional analysis of the ribonucleotide reductase genes in shrimp white spot syndrome virus

    NARCIS (Netherlands)

    Tsai, M.F.; Lo, C.F.; Hulten, van M.C.W.; Tzeng, H.F.; Chou, C.M.; Huang, C.J.; Wang, C.S.

    2000-01-01

    The causative agent of white spot syndrome (WSS) is a large double-stranded DNA virus, WSSV, which is probably a representative of a new genus, provisionally called Whispovirus. From previously constructed WSSV genomic libraries of a Taiwan WSSV isolate, clones with open reading frames (ORFs) that e

  14. BloodSpot: a database of gene expression profiles and transcriptional programs for healthy and malignant haematopoiesis

    DEFF Research Database (Denmark)

    Bagger, Frederik Otzen; Sasivarevic, Damir; Hadi Sohi, Sina

    2016-01-01

    largely inaccessible. Current databases provide information about gene-expression but fail to answer key questions regarding co-regulation, genetic programs or effect on patient survival. To address these shortcomings, we present BloodSpot (www.bloodspot.eu), which includes and greatly extends our...... previously released database HemaExplorer, a database of gene expression profiles from FACS sorted healthy and malignant haematopoietic cells. A revised interactive interface simultaneously provides a plot of gene expression along with a Kaplan–Meier analysis and a hierarchical tree depicting...... the relationship between different cell types in the database. The database now includes 23 high-quality curated data sets relevant to normal and malignant blood formation and, in addition, we have assembled and built a unique integrated data set, BloodPool. Bloodpool contains more than 2000 samples assembled from...

  15. Influence of white spot syndrome virus infection on hepatopancreas gene expression of `Huanghai No. 2' shrimp ( Fenneropenaeus chinensis)

    Science.gov (United States)

    Meng, Xianhong; Shi, Xiaoli; Kong, Jie; Luan, Sheng; Luo, Kun; Cao, Baoxiang; Liu, Ning; Lu, Xia; Li, Xupeng; Deng, Kangyu; Cao, Jiawang; Zhang, Yingxue; Zhang, Hengheng

    2017-10-01

    To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus (WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of `Huanghai No. 2' ( Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early (48-96 h), peak (168-192 h) and late (264-288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes (1916 annotated) expressed at all three phases, and most of the annotated were either up- or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV.

  16. BloodSpot: a database of gene expression profiles and transcriptional programs for healthy and malignant haematopoiesis

    DEFF Research Database (Denmark)

    Bagger, Frederik Otzen; Sasivarevic, Damir; Hadi Sohi, Sina;

    2016-01-01

    largely inaccessible. Current databases provide information about gene-expression but fail to answer key questions regarding co-regulation, genetic programs or effect on patient survival. To address these shortcomings, we present BloodSpot (www.bloodspot.eu), which includes and greatly extends our...... the relationship between different cell types in the database. The database now includes 23 high-quality curated data sets relevant to normal and malignant blood formation and, in addition, we have assembled and built a unique integrated data set, BloodPool. Bloodpool contains more than 2000 samples assembled from...

  17. Self-assembly of various Au nanocrystals on functionalized water-stable PVA/PEI nanofibers: a highly efficient surface-enhanced Raman scattering substrates with high density of "hot" spots.

    Science.gov (United States)

    Zhu, Han; Du, MingLiang; Zhang, Ming; Wang, Pan; Bao, ShiYong; Zou, Meiling; Fu, YaQin; Yao, JuMing

    2014-04-15

    We have demonstrated a facile approach for the fabrication of flexible and reliable sulfydryl functionalized PVA/PEI nanofibers with excellent water stability for the self-assembly of Au nanocrystals, such as Au nanoparticles (AuNPs), Au nanoflowers (AuNFs) and Au nanorods (AuNRs), used as the highly efficient surface-enhanced Raman scattering (SERS) substrates for the detection of rhodamine B (RhB). Various methods were employed to cross-link the PVA nanofibers with better morphology and porous structures after immersing in water for desired times. Various SERS-active Au nanocrystals, such as AuNPs, AuNFs, and AuNRs have been successfully synthesized. After the grafting of MPTES on the cross-linked PVA/PEI nanofibers, the Au nanocrystals can easily be self-assembled on the surfaces of the nanofibers because of the strong interactions of the Au-S chemical bondings. The Au nanocrystals self-assembled throughout the PVA/PEI nanofibers used as SERS substrates all exhibit enhanced SERS signals of RhB compared with their individual nanocrystals. It is mainly due to the close interparticle distance, mutual orientation and high density of "hot" spots, that can strongly affect the overall optical response and the SERS enhancement. By changing the amounts of the self-assembled AuNFs on the nanofibers, we can control the density of the "hot" spots. With the increased amounts of the AuNFs throughout the nanofibers, the SERS substrates show enhanced Raman signals of the RhB, indicating that the increased density of "hot" spots can directly lead to the SERS enhancement. The AuNFs/(PVA/PEI) SERS substrates show good sensitivity, reliability and low detection limit (10(-9) M). The presented approach can be broadly applicable to the assembly of different types of plasmonic nanostructures and these novel materials with strong SERS enhancement can be applied in bioanalysis and biosensors.

  18. Research on the Dynamic Template Matching Method in Solar Energy Component Hot Spot Detection%太阳能组件热斑检测中的动态模板匹配方法研究

    Institute of Scientific and Technical Information of China (English)

    王阿勇; 杨静

    2012-01-01

    In order to decrease costs and effectively improve the component production qualities, thermal imagery is used to cany out the hot spot detection in solar energy components. The thermal images of solar energy components of large plate types are obtained in two times, and then they are spliced. In splicing thermal images, this paper suggests a splice algorithm based on the dynamic plate thermal images using the periodical properties shown by the component thermal images. Through corresponding tests, it can be proved that the algorithm can effectively improve component' s hot spot on-line detection efficiency. The designed detection scheme is adaptable to automatic hot spot detection with different specifications.%为了降低成本,有效提高组件的生产质量,利用热图像进行太阳能组件的热斑检测.分两次获取大板型太阳能组件热图像,然后进行图像拼接.在热图像的拼接中,利用组件热图像呈现周期性的特征,提出基于动态模板的热图像拼接算法.经过测试,动态模板的热图像拼接算法有效地提高了组件热斑在线检测效率,设计的检测方案适用于不同规格组件的热斑自动检测.

  19. Evaluation of Caspofungin Susceptibility Testing by the New Vitek 2 AST-YS06 Yeast Card Using a Unique Collection of FKS Wild-Type and Hot Spot Mutant Isolates, Including the Five Most Common Candida Species

    DEFF Research Database (Denmark)

    Astvad, Karen M; Perlin, David S; Johansen, Helle K

    2013-01-01

    FKS mutant isolates associated with breakthrough or failure cases are emerging in clinical settings. Discrimination of these from wild-type (wt) isolates in a routine laboratory setting is complicated. We evaluated the ability of caspofungin MIC determination using the new Vitek 2 AST-Y06 yeast...... susceptibility card to correctly identify the fks mutants from wt isolates and compared the performance to those of the CLSI and EUCAST reference methods. A collection of 98 Candida isolates, including 31 fks hot spot mutants, were included. Performance was evaluated using the FKS genotype as the "gold standard...

  20. Surface-Enhanced Raman Spectroscopy of Dye and Thiol Molecules Adsorbed on Triangular Silver Nanostructures: A Study of Near-Field Enhancement, Localization of Hot-Spots, and Passivation of Adsorbed Carbonaceous Species

    Directory of Open Access Journals (Sweden)

    Manuel R. Gonçalves

    2012-01-01

    Full Text Available Surface-enhanced Raman spectroscopy (SERS of thiols and dye molecules adsorbed on triangular silver nanostructures was investigated. The SERS hot-spots are localized at the edges and corners of the silver triangular particles. AFM and SEM measurements permit to observe many small clusters formed at the edges of triangular particles fabricated by nanosphere lithography. Finite-element calculations show that near-field enhancements can reach values of more than 200 at visible wavelengths, in the gaps between small spherical particles and large triangular particles, although for the later no plasmon resonance was found at the wavelengths investigated. The regions near the particles showing strong near-field enhancement are well correlated with spatial localization of SERS hot-spots done by confocal microscopy. Silver nanostructures fabricated by thermal evaporation present strong and fast fluctuating SERS activity, due to amorphous carbon contamination. Thiols and dye molecules seem to be able to passivate the undesired SERS activity on fresh evaporated silver.

  1. 多层套管燃料元件工程热点因子敏感性分析%Sensitivity Analysis of Engineering Hot Spot Factor for Multi-layer Tube Fuel Element

    Institute of Scientific and Technical Information of China (English)

    胡跃春; 邓才玉; 李海涛; 徐涛忠

    2014-01-01

    为保证反应堆的安全,并对燃料元件的制造加工提出合理可行的要求,从元件制造加工和反应堆运行测量两方面对多层套管燃料元件工程热点因子的敏感性进行了分析。结果表明:流道间隙偏差直接影响元件热源的导出,由此引起的工程热点因子造成的温升较大。%For ensuring reactor safety and putting forward reasonable requirements of fuel element manufacture , the sensitivity analysis of engineering hot spot factor for multi-layer tube fuel element was completed from both aspects of fuel manufacture and reactor operation measurement .The result shows that the flow channel deviation is of direct effect on fuel element heat transfer ,yielding a higher temperature rise caused by the relevant engineering hot spot factor .

  2. HOT SPOT STRESS ANALYSIS OF DIFFERENT GIRTH BUTT WELD DETAILS OF CAST STEEL JOINT%不同焊接构造的铸钢节点环形对接焊缝的热点应力分析

    Institute of Scientific and Technical Information of China (English)

    韩庆华; 郭琪; 林允昶; 邢颖

    2014-01-01

    铸钢节点与主体构件的环形对接焊缝处存在材料差异、几何差异和焊接残余应力,因此环形对接焊缝是铸钢节点疲劳破坏的重要研究对象。目前,国内对铸钢节点焊缝疲劳问题的研究比较缺乏,传统名义应力方法并不适用于铸钢节点。采用热点应力方法对铸钢节点环形对接焊缝的疲劳问题进行探究,比较4种热点应力外推方法的计算结果。为减小铸钢节点与主体构件不等壁厚对接焊缝产生的应力集中对疲劳性能的影响,分析对比了3种不同的焊接构造,并分别研究在壁厚t、径厚比d/t、壁厚比T/t三种参数影响下的热点应力变化规律。%The girth butt weld attaching to the cast steel joint and the hot-rolled tube are the important object of the fatigue study of the cast steel joint due to the material nonlinearity and geometric nonlinearity across the weld . However , the research on this issue rarely saw in China and the nominal stress method was not applicable to it .In the paper, the hot spot stress method was used while the results of hot spot stress extrapolations were compared .To reduce the impact of the stress concentration , different weld details were designed .The hot spot stress curve was drawn under consideration of the tube ’ s thickness and the other two parameters .

  3. Evaluation of Genetic Diversity Using Parameters Based on Probability of Gene Origin in the Slovak Spotted Bulls

    Directory of Open Access Journals (Sweden)

    E. Hazuchová

    2012-05-01

    Full Text Available The aim of this study was to assess the diversity based on probability of gene origin in Slovak Spotted bulls. The pedigree information was available from The Breeding Services of the Slovak Republic, s. e. The pedigree file consisted of 752 individuals. The 62 sires born from 1995 to 2009 and registered in Herd book set up the analyzed reference (RP population. Total number of founders in the RP was 308, effective number of founders was 115 and the effective number of ancestors was 37. The number of ancestors explaining 50 % of the diversity was 15 and founder’s genome equivalent was 20.46. The sire GS Malf and Horwein were with 16 offspring’s the most frequently used bulls in the artificial insemination. We found that the genetic conservation index for RP was 16.34 %. Results will be used in genetic management of breeding work in Slovak Spotted and monitoring of parameters characterizing genetic diversity and their development, as well.

  4. A literature review of epidemiological studies on mutation hot spots of Chinese population with non-syndromic hearing loss%非综合征性聋突变热点的流行病学分析

    Institute of Scientific and Technical Information of China (English)

    李海波; 李琼; 李红; 陈瑛

    2012-01-01

    Objective: To identify the regional mutation hot spots characteristics, and then to establish a regional genetic screening programs. Method; Molecular epidemiological literatures about Chinese deafness gene GJB2,SLC26A4,mitochondrial DNA mutations from 2006 to the first half of 2011 were retrieved in Wanfang and Pubmed literature database. The primary data of these studies including the number of samples, demographic characteristics, mutation frequencies and so on,were analyzed statiscally. Result:Of 46 papers, 42 were included in this study. The patients all had non-syndromic sensorineural hearing loss and lived in 20 regions of China. A total of 18094 were counted and the average mutation frequencies were approximately 42.0%. The mutation frequencies of 235 delC were 16. 34%, 299-300 delAT were 4. 75% in GJB2 gene and IVS7-2A>G were 12. 60% , 2168A>G were 2. 32% in SLC26A4 gene and the mutation frequencies of 1555A>G, 1494C>T in mtDNA were 5. 21 %, 1. 11% respectively. The statistical discrepancy was significant among mutation frequencies in different regions by χ2test(P<0. 05). Conclusion:Molecular epidemiological statistics show that the 6 locus are the mutation hot spots in Chinese people with non-syndromic hearing loss and can be used for genetic screening according to the specific regional mutation characteristics.%目的:了解耳聋基因突变热点在中国患者中的分布特征,以建立针对突变热点的区域性基因筛查方案.方法:运用万方和Pubmed等检索2006-2011年上半年国内报道的中国各地区人群耳聋相关基因GJB2、SLC26A4、mtDNA突变流行病学文献,对文献中的样本量、样本特征、地域分布、突变频率等多个因素进行统计分析.结果:检索到相关文献46篇,纳入该统计分析42篇,研究区域涉及中国20个省、市、自治区和直辖市,调查人群均为非综合征型感音神经性聋患者;样本总量18 094例,GJB2 235 delC突变频率为16.34%,GJB2 299-300 del

  5. Identification of the Bcl-2 family protein gene BOK from orange-spotted grouper (Epinephelus coioides) involved in SGIV infection.

    Science.gov (United States)

    Cai, Jia; Yu, Dapeng; Wei, Shina; Tang, Jufen; Lu, Yishan; Wu, Zaohe; Qin, Qiwei; Jian, Jichang

    2016-05-01

    Apoptosis plays vital roles in many physiological process and immune response. BOK is one of the central regulators in apoptosis. In this study, a new BOK homolog (Ec-BOK) was cloned and characterized from Orange-spotted grouper, Epinephelus coioides. Ec-BOK encoded a 210 amino acid peptides which shared 97% identity to Stegastes partitus BOK protein, contained four BH domains and one transmembrane region. Ec-BOK widely expressed in all analyzed tissues with the higher expressions in kidney and spleen. Its expression level was up-regulated after SGIV infection in vitro. Further analysis revealed that overexpression of Ec-BOK inhibited viral genes transcriptions and virus replication in fish cell. Our findings suggested that Ec-BOK might play a role in the immune response against virus.

  6. Cathepsin L in the orange-spotted grouper, Epinephelus coioides: molecular cloning and gene expression after a Vibrio anguillarum challenge.

    Science.gov (United States)

    Liang, Jing-Zhen; Rao, Ying-Zhu; Lun, Zhao-Rong; Yang, Ting-Bao

    2012-12-01

    The orange-spotted grouper, Epinephelus coioides, is an important fish maricultured in many Asian countries. In the present study, the full-length cDNA of cathepsin L, an immunity related gene of fishes, was isolated from E. coioides using rapid amplification of cDNA ends (RACE). It is 1,443 bp in length, including an open reading frame (ORF) of 1,011 bp. The open reading frame encoded a preproprotein of 336 amino acids (aa), which consisted of a signal peptide of 16 aa, a proregion peptide of 98 aa and a mature peptide of 222 aa. The preproprotein contained an oxyanion hole (Gln), a catalytic triad formed by Cys, His and Asn, and the conserved ERWNIN, GNFD and GCNGG motifs, all characteristic of cathepsin L. Homology analysis revealed that the deduced amino acid sequence of E. coioides cathepsin L shared 80.1-94.8 % identity with those of reported fishes. Tissue-dependent mRNA expression analysis showed that the cathepsin L transcript was expressed in all the examined tissues of the healthy E. coioides, being highest in the liver and moderate in the heart, gonad and intestine. After Vibrio anguillarum stimulation, the mRNA expression of cathepsin L in E. coioides was significantly increased in the skin, fin, gills, liver, blood, spleen, head kidney and intestine, with the highest observed in the spleen (10.6-fold) at 12 h post-injection and the next in blood (7.5-fold) at 8 h post-injection. These results provided initial information for further studies on the physiological and immunological roles of the cathepsin L gene in the orange-spotted grouper.

  7. Two variants in the KIT gene as candidate causative mutations for a dominant white and a white spotting phenotype in the donkey.

    Science.gov (United States)

    Haase, B; Rieder, S; Leeb, T

    2015-06-01

    White spotting phenotypes have been intensively studied in horses, and although similar phenotypes occur in the donkey, little is known about the molecular genetics underlying these patterns in donkeys. White spotting in donkeys can range from only a few white areas to almost complete depigmentation and is characterised by a loss of pigmentation usually progressing from a white spot in the hip area. Completely white-born donkeys are rare, and the phenotype is characterised by the complete absence of pigment resulting in pink skin and a white coat. A dominant mode of inheritance has been demonstrated for spotting in donkeys. Although the mode of inheritance for the completely white phenotype in donkeys is not clear, the phenotype shows similarities to dominant white in horses. As variants in the KIT gene are known to cause a range of white phenotypes in the horse, we investigated the KIT gene as a potential candidate gene for two phenotypes in the donkey, white spotting and white. A mutation analysis of all 21 KIT exons identified a missense variant in exon 4 (c.662A>C; p.Tyr221Ser) present only in a white-born donkey. A second variant affecting a splice donor site (c.1978+2T>A) was found exclusively in donkeys with white spotting. Both variants were absent in 24 solid-coloured controls. To the authors' knowledge, this is the first study investigating genetic mechanisms underlying white phenotypes in donkeys. Our results suggest that two independent KIT alleles are probably responsible for white spotting and white in donkeys.

  8. Investigating the origin of cyclical wind variability in hot massive stars - II. Hydrodynamical simulations of corotating interaction regions using realistic spot parameters for the O giant ξ Persei

    Science.gov (United States)

    David-Uraz, A.; Owocki, S. P.; Wade, G. A.; Sundqvist, J. O.; Kee, N. D.

    2017-09-01

    OB stars exhibit various types of spectral variability historically associated with wind structures, including the apparently ubiquitous discrete absorption components (DACs). These features have been proposed to be caused either by magnetic fields or non-radial pulsations. In this second paper of this series, we revisit the canonical phenomenological hydrodynamical modelling used to explain the formation of DACs by taking into account modern observations and more realistic theoretical predictions. Using constraints on putative bright spots located on the surface of the O giant ξ Persei derived from high precision space-based broad-band optical photometry obtained with the Microvariability and Oscillations of Stars (MOST) space telescope, we generate 2D hydrodynamical simulations of corotating interaction regions in its wind. We then compute synthetic ultraviolet (UV) resonance line profiles using Sobolev Exact Integration and compare them with historical timeseries obtained by the International Ultraviolet Explorer (IUE) to evaluate if the observed behaviour of ξ Persei's DACs is reproduced. Testing three different models of spot size and strength, we find that the classical pattern of variability can be successfully reproduced for two of them: the model with the smallest spots yields absorption features that are incompatible with observations. Furthermore, we test the effect of the radial dependence of ionization levels on line driving, but cannot conclusively assess the importance of this factor. In conclusion, this study self-consistently links optical photometry and UV spectroscopy, paving the way to a better understanding of cyclical wind variability in massive stars in the context of the bright spot paradigm.

  9. Neurofibromatosis type 1 (NF1) gene: Beyond café au lait spots and dermal neurofibromas.

    Science.gov (United States)

    Peltonen, Sirkku; Kallionpää, Roope A; Peltonen, Juha

    2016-09-13

    Neurofibromatosis 1 (NF1) occurs in 1:2000 births. The main diagnostic signs are visible on the skin, and this opens several interesting aspects for dermatological point of view. The NF1 syndrome is caused by mutations in the NF1 gene which encodes the tumor suppressor protein neurofibromin. Neurofibromin functions as a Ras-GTPase-activating protein (RasGAP), and NF1 mutations lead to overactivation of the Ras signalling pathway. The NF1 gene and neurofibromin have intriguing functions in keratinocytes and melanocytes. Neurofibromin regulates melanin synthesis and keratinocyte differentiation in a currently unknown manner. The NF1 gene has also an important but poorly understood role in tumorigenesis and cancer. Compared to the general population, NF1 patients have a fivefold risk for cancer and a more than 2000-fold risk for neurogenic malignancies. Mutations of the NF1 gene are common in numerous cancer types in patients without NF1, and this suggests a more general role for the NF1 gene in oncogenesis. In melanoma, NF1 mutations seem to drive tumorigenesis and contribute to drug resistance. In this article, we review the literature on neurofibromin with special attention to keratinocytes, melanocytes, NF1-related tumors and melanoma.

  10. First report of a resistance-breaking strain of Tomato spotted wilt virus infecting tomatoes with the Sw-5 tospovirus-resistance gene in California

    Science.gov (United States)

    Management of Tomato spotted wilt virus (TSWV) with the Sw-5 resistance gene in tomato is highly effective. However, in certain regions of the world where resistant tomatoes have been continually planted, resistance-breaking strains of TSWV have emerged. In spring 2016 resistant tomatoes were obse...

  11. Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277

    Science.gov (United States)

    The Andean common bean (Phaseolus vulgaris L.) cultivar AND 277 is an important source of disease resistance to angular leaf spot (ALS, caused by Pseudocercospora griseola) and anthracnose (caused by Colletotrichum lindemuthianum). AND 277 has the Phg-1 gene that confers resistance to eight races of...

  12. Molecular characterization and expression analysis of four isotypes of immunoglobulin light chain genes in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wu, Ming-Shan; Cheng, Chao-An; Lin, Chih-Hung; Lee, Chiou-Yueh; Tseng, Shih-Jou; Tzeng, Chyng-Shyan; Chang, Chi-Yao

    2013-03-01

    To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identification of 26 full Ig light chain (OsgIgL) coding sequences. These 26 OsgIgLs encoded peptides from 235 to 248 amino acid residues and could be grouped into five variable (V(L)) and four constant (C(L)) isotypes. The C(L) regions contained three conserved cysteine residues that may participate in intra- or inter-chain disulfide bond formation. The four C(L) isotypes could be sub-grouped into two serological types: κ (C(L)-I, C(L)-II and C(L)-III) and σ (C(L)-IV), by phylogenetic analysis. The OsgIgL genes were found to be expressed in various tissues, with greatest levels of expression observed in the head-kidney and spleen. The major expression type was C(L)-I, which comprised 92% and 91% of total OsgIgL gene expression in the head-kidney and spleen, respectively. Transcription of all four C(L) isotypes was differentially affected in response to various immunostimulators, including lipopolysaccharide (LPS), poly I:C and grouper iridovirus (GIV). Induction of OsgIgL genes in response to immunostimulators was particularly dramatic in the spleen, suggesting this organ holds particular importance for the regulation of OsgIgL expression. Furthermore, vaccination of grouper with formalin-inactivated GIV also induced differential patterns of expression in all four OsgIgL isotypes. In summary, the significant and diverse patterns of transcriptional induction observed for OsgIgL isotypes in the spleen and head-kidney imply that each isotype may have unique roles in the immune response.

  13. The T945M Single Nucleotide Polymorphism of the Bovine Leptin Receptor Gene in Population of Slovak Spotted Bulls

    Directory of Open Access Journals (Sweden)

    Anna Trakovická

    2011-09-01

    Full Text Available The objective of this study was detection of DNA polymorphism of the leptin receptor gene using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP. The SNP T945M, which maps on bovine chromosome 3 at the exon 20 of the leptin receptor sequence and corresponds to a mutation in the intracellular region of the functional protein, was also analyzed. In exon 20, a T to C missense mutation was found at nucleotide 115, which causes an amino acid substitution at residue 945 (T954M. The polymorphism of leptin receptor gene was studied in a group of 57 bulls of Slovak spotted breed. A strategy employing PCR was used to amplify 197 bp products from blood samples. Digestion of PCR products with restriction enzyme BseGI revealed two alleles: allele C was 130 and 67 fragments and allele T was 93, 67 and 37. Frequencies for allele C and T were 0.9737 and 0.0263, respectively and TT genotype was not detected.

  14. Spatiotemporal distribution of the seismicity along the Mid-Atlantic Ridge north of the Azores from hydroacoustic data: Insights into seismogenic processes in a ridge-hot spot context

    Science.gov (United States)

    Goslin, J.; Perrot, J.; Royer, J.-Y.; Martin, C.; LourençO, N.; Luis, J.; Dziak, R. P.; Matsumoto, H.; Haxel, J.; Fowler, M. J.; Fox, C. G.; Lau, A. T.-K.; Bazin, S.

    2012-02-01

    The seismicity of the North Atlantic was monitored from May 2002 to September 2003 by the `SIRENA array' of autonomous hydrophones. The hydroacoustic signals provide a unique data set documenting numerous low-magnitude earthquakes along the section of the Mid-Atlantic Ridge (MAR) located in a ridge-hot spot interaction context. During the experiment, 1696 events were detected along the MAR axis between 40°N and 51°N, with a magnitude of completeness level ofmb≈ 2.4. Inside the array, location errors are in the order of 2 km, and errors in the origin time are less than 1 s. From this catalog, 15 clusters were detected. The distribution of source level (SL) versus time within each cluster is used to discriminate clusters occurring in a tectonic context from those attributed to non-tectonic (i.e. volcanic or hydrothermal) processes. The location of tectonic and non-tectonic sequences correlates well with regions with positive and negative Mantle Bouguer Anomalies (MBAs), indicating the presence of thinner/colder and thicker/warmer crust respectively. At the scale of the entire array, both the complete and declustered catalogs derived from the hydroacoustic signals show an increase of the seismicity rate from the Azores up to 43°30'N suggesting a diminishing influence of the Azores hot spot on the ridge-axis temperature, and well correlated with a similar increase in the along-axis MBAs. The comparison of the MAR seismicity with the Residual MBA (RMBA) at different scales leads us to think that the low-magnitude seismicity rates are directly related to along-axis variations in lithosphere rheology and temperatures.

  15. 肺癌患者SPECT/CT同机融合显像肋骨单发病灶影像分析%SPECT/CT fusion imaging analysis of costal single hot spots in patients with lung cancer

    Institute of Scientific and Technical Information of China (English)

    包贺菊; 陈刚; 陈燕燕

    2014-01-01

    Objective To analyze the imaging features and clinical significance of solitary costal hot spots in patient with lung cancer on SPECT/CT fusion imaging and the value of low-dose CT for diagnosis.Methods SPECT/CT scan was performed on 68 patients with lung cancer and costal single hot spots using whole body bone imaging.The sites of lesions were classified into four types:junction between costal cartilage and rib,frontal rib,lateral rib,posterior rib.The shapes of lesions were classified into two forms:punctiform and strip shapes,and were analyzed and classified.The differences of the diagnosis of whole body bone imaging and SPECT/CT scan were analyzed.Results Punctiform spots were mainly due to costal fractures(36/68,52.94%).The majority of spots in strip shape were costal metastases (21/23,91.30%).The lesions of junction between costal cartilage and rib were punctiform shape and benign.Hot spots in posterior ribs were mainly costal metastases (18/22,81.82%).Sensitivity of diagnosis of the metastatic bone tumor with whole body bone imaging and SPECT/CT in single hot spot of spine and rib was 69.23% (18/26) and 92.31%(24/26),specificity 59.52%(25/42) and 85.71%(36/42),accuracy 63.24%(43/68) and 88.24% (60/68),positive predictive value 51.43% (18/35) and 80.00% (24/30),negative predictive value 75.76% (25/33) and 94.74% (36/38).SPECT/CT scan had differences with whole body bone imaging in sensitivity (P < 0.05),SPECT/CT scan had significant differences with whole body bone imaging in coincidence and specificity (P < 0.01).Conclusions Useful imaging features can be obtained from SPECT/CT fusion imaging with single costal hot spots,being helpful to differential diagnosis of benign and malignant lesions.SPECT/CT fusion imaging may improve the accuracy to diagnose the rib disease.%目的 分析肺癌患者SPECT/CT同机融合显像肋骨单发病灶的特征和临床诊断之间的相互关系,并评价定位CT在诊断中的作用.方法

  16. Hot spot stress analysis of girth butt weld in cast steel joint%铸钢节点环形对接焊缝热点应力分析

    Institute of Scientific and Technical Information of China (English)

    韩庆华; 陈志钢; 芦燕

    2011-01-01

    铸钢节点的壁厚大于连接杆件,两者连接时壁厚方向存在几何形状的突变,引起焊缝附近的应力集中,降低其疲劳性能。目前现行规程或标准针对铸钢节点与主体结构连接处焊缝的疲劳问题没有明确的设计规定。本文选取两种焊接构造,采用热点应力的表面线性外推法对环形对接焊缝进行轴力作用下的疲劳性能有限元分析。同时选取结构杆件的壁厚t、径厚比d/t以及铸钢与普通杆件的壁厚比T/t作为热点应力的影响因素进行参数分析。分析结果表明:热点应力与厚度t关系不大,随着ln(d/t)的增大热点应力呈线性增加;随着T/t的增加,热点应力先增大后减小;ln(σ/(T/t))随着T/t的增加呈线性减小。%As cast steel joints are thicker than the hot-rolled tubes attached,sharp transition in the direction of thickness would cause stress concentration and weaken its fatigue performance.At present,there are no regulations or standards to guide the design of girth butt weld's fatigue behavior of cast steel joints.A hot-spot stress FEM analysis of weld's fatigue behavior was conducted based on the surface extrapolation method in this paper,considering two kinds of welding details under axial loads.Parametric analysis were carried out on the thickness(t) of hot-rolled tube,the ratio of tube's diameter to its thickness(d/t) and the ratio of cast steel joint's thickness to tube's(T/t).The results show that although the hot-spot stress is independent of the thickness of tubes(t),it will increase linearly with the increment of ln(d/t) and increase firstly then decrease with the increment of T/t.In addition,ln(σ/(T/t))(σ means the hot-spot stress) decreases linearly with the increment of T/t.

  17. Molecular cloning and characterization of c-type lysozyme gene in orange-spotted grouper, Epinephelus coioides.

    Science.gov (United States)

    Wei, Shina; Huang, Youhua; Cai, Jia; Huang, Xiaohong; Fu, Jing; Qin, Qiwei

    2012-08-01

    Lysozymes are key proteins of the host innate immune system against pathogen infection. In this study, a c-type lysozyme gene (Ec-lysC) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysC cDNA is composed of 533 bp and encodes a polypeptide of 144-residue protein with 94% identity to lysC of Kelp grouper, Epinephelus bruneus. The genomic DNA of Ec-lysC consists of 4 exons and 3 introns, with a total length of 1897 bp. Amino acid sequence alignment showed that Ec-lysC possessed conserved catalytic residues (Glu50 and Asp67) and "GSTDYGIFQINS" motif. RT-PCR results showed that Ec-lysC transcript was most abundant in head kidney and less in muscle. The expression of Ec-lysC was differentially up-regulated in head kidney after stimulation with lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-lysC was distributed predominantly in the cytoplasm. The recombinant Ec-lysC (rEc-lysC) had lytic activities against Gram-positive bacteria Micrococcus lysodeikticus, Staphylococcus aureus, Streptococcus iniae and Gram-negative bacteria V. alginolyticus. The lysozyme acted on M. lysodeikticus cell walls as shown by scanning electron microscopy (SEM). Furthermore, overexpression of Ec-lysC in grouper cells delayed the occurrence of CPE induced by SGIV and inhibited the viral gene transcription significantly. Taken together, Ec-lysC might play an important role in grouper innate immune responses to invasion of bacterial and viral pathogens. C-type lysozyme gene from E. coioides (Ec-lysC) was identified and characterized.

  18. Regional Fluctuation in the Functional Consequence of LINE-1 Insertion in the Mitf Gene: The Black Spotting Phenotype Arisen from the Mitfmi-bw Mouse Lacking Melanocytes.

    Science.gov (United States)

    Takeda, Kazuhisa; Hozumi, Hiroki; Ohba, Koji; Yamamoto, Hiroaki; Shibahara, Shigeki

    2016-01-01

    Microphthalmia-associated transcription factor (Mitf) is a key regulator for differentiation of melanoblasts, precursors to melanocytes. The mouse homozygous for the black-eyed white (Mitfmi-bw) allele is characterized by the white-coat color and deafness with black eyes due to the lack of melanocytes. The Mitfmi-bw allele carries LINE-1, a retrotransposable element, which results in the Mitf deficiency. Here, we have established the black spotting mouse that was spontaneously arisen from the homozygous Mitfmi-bw mouse lacking melanocytes. The black spotting mouse shows multiple black patches on the white coat, with age-related graying. Importantly, each black patch also contains hair follicles lacking melanocytes, whereas the white-coat area completely lacks melanocytes. RT-PCR analyses of the pigmented patches confirmed that the LINE-1 insertion is retained in the Mitf gene of the black spotting mouse, thereby excluding the possibility of the somatic reversion of the Mitfmi-bw allele. The immunohistochemical analysis revealed that the staining intensity for beta-catenin was noticeably lower in hair follicles lacking melanocytes of the homozygous Mitfmi-bw mouse and the black spotting mouse, compared to the control mouse. In contrast, the staining intensity for beta-catenin and cyclin D1 was higher in keratinocytes of the black spotting mouse, compared to keratinocytes of the control mouse and the Mitfmi-bw mouse. Moreover, the keratinocyte layer appears thicker in the Mitfmi-bw mouse, with the overexpression of Ki-67, a marker for cell proliferation. We also show that the presumptive black spots are formed by embryonic day 15.5. Thus, the black spotting mouse provides the unique model to explore the molecular basis for the survival and death of developing melanoblasts and melanocyte stem cells in the epidermis. These results indicate that follicular melanocytes are responsible for maintaining the epidermal homeostasis; namely, the present study has provided

  19. The bacteriophage P1 hot gene, encoding a homolog of the E. coli DNA polymerase III theta subunit, is expressed during both lysogenic and lytic growth stages.

    Science.gov (United States)

    Chikova, Anna K; Schaaper, Roel M

    2007-11-01

    The bacteriophage P1 hot gene product is a homolog of the theta subunit of E. coli DNA polymerase III. Previous studies with hot cloned on a plasmid have shown that Hot protein can substitute for theta, as evidenced by its stabilizing effect on certain dnaQ mutator mutants carrying an unstable pol III proofreading subunit (epsilon subunit). These results are consistent with Hot, like theta, being a replication protein involved in stabilizing the intrinsically unstable epsilon proofreading function. However, the function of hot for the viral life cycle is less clear. In the present study, we show that the hot gene is not essential. Based on its promoter structure, hot has been previously classified as a "late" phage gene, a property that is not easily reconciled with a presumed replication function. Here, we clarify this issue by demonstrating that P1 hot is actively expressed both during the lysogenic state and in the early stages of a lytic induction, in addition to its expression in the late stage of phage development. The results indicate that P1 hot has a complex expression pattern, compatible with a model in which Hot may affect the host replication machinery to benefit overall phage replication.

  20. 以箱壁温度为判据的油浸式变压器绕组热点温度计算模型及试验分析%Model of Hot Spot Temperature in Oil-immersed Transformers Using Temperature Estimation of Tank Wall

    Institute of Scientific and Technical Information of China (English)

    李剑; 刘兴鹏; 王有元; 陈伟根; 邓宗权

    2011-01-01

    电力变压器的热点温度是影响其绝缘寿命非常重要的因素,准确估计电力变压器热点温度,有助于提高电力变压器的经济与安全运行水平。为此,提出了一种估计变压器绕组热点温度的改进方法。该方法依据变压器热路模型,采用变压器油箱壁温度来估计绕组热点温度。为了验证该方法的准确性,在实验室的试验变压器上进行了负载试验,得到不同负载下的变压器绕组热点温度和油箱壁温度的实测值,并将热点温度的实测结果与该方法计算值以及IEEE负载导则公式的计算值进行了比较。分析结果表明,改进方法具有较小的热点温度估计误差,证明了改进方法估计变压器热点温度的有效性。%Hot spot temperature is a very important factor that influences lifetime of insulation in power transtormers. Accurate estimation of the hot spot temperatures of transformer windings is helpful to improving economic and safe operation levels of transformers. Consequently, an improved approach was presented to estimate hot spot temperatures of transformer windings. The improved approach was based on a thermal circuit of power transformers. Temperatures of transformer wails were used to estimate the hot spot temperature of transformer windings, and load of transformers was tested in laboratory to obtain the measurement data of temperatures of hot spot and transformer walls. The measurement results were compared with the estimated results of the improved approach and the calculated results of IEEE loading guide equations. The analysis results show that the improved approach has smaller estimation errors of hot spot temperature than the IEEE load guide equations. The improved approach is verified to be effective for estimation of hot spot temperature in transformers.

  1. Acclimation of killifish to thermal extremes of hot spring: Transcription of gonadal and liver heat shock genes.

    Science.gov (United States)

    Akbarzadeh, Arash; Leder, Erica H

    2016-01-01

    In this study, we explored the hypothesis that killifish acclimate to thermal extremes through regulation of genes involved in stress and metabolism. We examined the liver and gonadal transcription of heat shock proteins (hsp70, hsp90a, hsp90b), glucokinase (gck), and high mobility group b1 (hmgb1) protein in wild killifish species from hot springs and rivers using quantitative real-time PCR. Moreover, we exposed a river killifish species to a long-term thermal regime of hot spring (37-40°C) and examined the liver transcription of the heat shock genes. Our results showed that hot spring killifish showed a significant, strong upregulation of liver hsp90a. Moreover, the testicular transcript levels of hsp90a, hsp90b, and hsp70 were higher in hot spring killifish than the river ones. The results of the common garden experiments showed that the transcripts of hsp70, hsp90b, and hmgb1 were mildly induced (> twofold) at the time when temperature reached to 37-40°C, while the transcripts of hsp90a were strongly induced (17-fold increase). The level of hsp90a was dramatically more upregulated when fish were maintained in thermal extreme (42-fold change higher than in ambient temperature). Moreover, a significant downregulation of gck transcripts was observed at the time when temperature was raised to 37-40°C (80-fold decrease) and during exposure to long-term thermal extreme (56-fold decrease). It can be concluded that the regulation of heat shock genes particularly hsp90a might be a key factor of the acclimation of fish to high temperature environments like hot springs.

  2. Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine.

    Science.gov (United States)

    Dan, Xue-Ming; Zhang, Tuan-Wei; Li, Yan-Wei; Li, An-Xing

    2013-03-01

    In order to elucidate the immune-protective mechanisms of inactivated Cryptocaryon irritans vaccine, different doses of C. irritans theronts were used to immunize orange-spotted grouper (Epinephelus coioides). We measured serum immobilization titer, blood leukocyte respiratory burst activity, serum alternative complement activity, and serum lysozyme activity weekly. In addition, the expression levels of immune-related genes such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), major histocompatibility complexes I and II (MHC I and II), and transforming growth factor-β1 (TGF-β1) were determined in spleen and gills. The results showed that the immobilization titer, respiratory burst activity, and alternative complement activity of immunized fish were significantly increased, and the levels of the last two immune parameters in the high-dose vaccine group were significantly higher than in the low-dose vaccine group. Serum lysozyme activity in the high-dose vaccine group was significantly higher than in the PBS control group. Vaccination also regulated host immune-related gene expression. For example, at 2- and 3- weeks post immunization, IL-1β expression in the high-dose vaccine group spleen was significantly increased. At 4-weeks post immunization, the fish were challenged with a lethal dose of parasite, and the survival rates of high-dose vaccine group, low-dose vaccine group, PBS control group, and adjuvant control group were 80%, 40%, 0%, and 10% respectively. These results demonstrate that inactivated C. irritans vaccination improves specific and nonspecific immune responses in fish, enhancing their anti-parasite ability. These effects are vaccine antigen dose-dependent.

  3. 非小细胞肺癌生物标志物新热点%New hot spots on biomarkers for non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    沈岚; 李子明; 陆舜

    2010-01-01

    As a hot topic, biomarkers can provide reliable evidence for the individualized treatment of non-small cell lung cancer. With the further study of molecular biology and development of new drugs,biomarkers have shown a broad prospect in clinical application. Combining with the research in recent years,this review describes the progress in biomarkers for non-small-cell lung cancer.%生物标志物是近年肺癌研究的热点之一,可为非小细胞肺癌(NSCLC)患者的个体化治疗提供可靠的依据.随着分子生物学研究的深入,其在临床上已显示出广阔的应用前景.文章结合近年来的研究对NSCLC生物标志物的新热点进行阐述.

  4. Liver spots

    Science.gov (United States)

    Liver spots are changes in skin color that occur in older skin. The coloring may be due to aging, exposure to the sun or other sources of ultraviolet light, or causes that are not known. Liver spots are very common after age 40. They occur ...

  5. 国外足球耐力热点研究及其变化趋势分析%Research on the Hot Spots of Football Endurance in Foreign Countries and the Analysis of Its Changing Trend

    Institute of Scientific and Technical Information of China (English)

    陈小虎; 黄竹杭; 李春满

    2016-01-01

    以Web of ScienceTM核心合集数据库中(SCIE、SSCI和 A&HCI )收录的1997—2015年以足球耐力为主题的488篇文献信息作为数据源,运用科学知识图谱技术分析国外足球耐力研究的国家、机构和作者分布,并通过解析当前足球耐力研究的关键词,揭示国外足球耐力研究的相关热点以及其变化趋势。研究发现:美国的发文量最大,但英国所发文章的中心性更高,发文的机构主要集中在高等院校;来自各高等院校的Karim Chamari、Anis Chaoua‐chi、Peter Krustrup等教授是研究足球耐力的突出作者;研究的热点主要包括运动训练、竞技表现、训练方法和测试方法的可靠性、训练负荷及运动员的训练反应等;变化趋势以运动员训练和竞技表现为主线,由运动员身体反应过渡到检验测试方法的可靠性,再由关注训练方法的有效性过渡到利用先进技术分析运动员在训练和比赛中的表现。现阶段的足球耐力研究多为方法性研究,研究大多基于足球训练或比赛中的外在表现形成,缺乏支撑训练和比赛的基础性理论研究。%Selecting the literature from Web of ScienceTM core collection database (SCIE ,and SSCI and A&HCI) ,and taking the 488 articles during 1997—2015 with the theme of foot‐ball endurance as data source ,using knowledge map technology to analysis the football endur‐ance research study of foreign countries ,institutions and the distribution of authors ,and by re‐solving the current football endurance research keywords ,reveal the abroad study on the endur‐ance of football‐related hot spots and trends .The result shows that United States is issued much more than other countries ,but the most centrality is England .The institutions of issued mainly concentrated in university ;the professor of Karim Chamari ,Anis Chaouachi ,and Peter Krustrup from respective university are the highlight author to research

  6. Comparative sequence analysis of Solanum and Arabidopsis in a hot spot for pathogen resistance on potato chromosome V reveals a patchwork of conserved and rapidly evolving genome segments

    Directory of Open Access Journals (Sweden)

    Bruggmann Rémy

    2007-05-01

    Full Text Available Abstract Background Quantitative phenotypic variation of agronomic characters in crop plants is controlled by environmental and genetic factors (quantitative trait loci = QTL. To understand the molecular basis of such QTL, the identification of the underlying genes is of primary interest and DNA sequence analysis of the genomic regions harboring QTL is a prerequisite for that. QTL mapping in potato (Solanum tuberosum has identified a region on chromosome V tagged by DNA markers GP21 and GP179, which contains a number of important QTL, among others QTL for resistance to late blight caused by the oomycete Phytophthora infestans and to root cyst nematodes. Results To obtain genomic sequence for the targeted region on chromosome V, two local BAC (bacterial artificial chromosome contigs were constructed and sequenced, which corresponded to parts of the homologous chromosomes of the diploid, heterozygous genotype P6/210. Two contiguous sequences of 417,445 and 202,781 base pairs were assembled and annotated. Gene-by-gene co-linearity was disrupted by non-allelic insertions of retrotransposon elements, stretches of diverged intergenic sequences, differences in gene content and gene order. The latter was caused by inversion of a 70 kbp genomic fragment. These features were also found in comparison to orthologous sequence contigs from three homeologous chromosomes of Solanum demissum, a wild tuber bearing species. Functional annotation of the sequence identified 48 putative open reading frames (ORF in one contig and 22 in the other, with an average of one ORF every 9 kbp. Ten ORFs were classified as resistance-gene-like, 11 as F-box-containing genes, 13 as transposable elements and three as transcription factors. Comparing potato to Arabidopsis thaliana annotated proteins revealed five micro-syntenic blocks of three to seven ORFs with A. thaliana chromosomes 1, 3 and 5. Conclusion Comparative sequence analysis revealed highly conserved collinear regions

  7. An improved dynamic model of transformer hot spot temperature based on top oil temperature%基于顶层油温的变压器绕组热点温度计算改进模型

    Institute of Scientific and Technical Information of China (English)

    陈伟根; 苏小平; 周渠; 潘翀; 谢波

    2012-01-01

    油浸式电力变压器绕组热点温度是影响变压器绝缘寿命的重要参量,与变压器顶层油温密切相关。考虑非线性热阻一变压器断路阻抗、油粘滞度以及绕组损耗随温度的变化,引入粘滞度与损耗关于温度变化的修正因子,提出一种基于顶层油温的变压器绕组热点温度改进模型。模型参数选用Levenberg-Marquardt算法进行估算。对比实验室温升用试验变压器实测数据,该模型在欠负载(90%)、额定负载(100%)以及过负载(110%)下,显示了较好的一致性,特别在动态负载下,能够较好地描述各暂态温度变化情况,为变压器绕组热点温度计算提出了一种新方法。%The winding hot spot temperature of the oil-immersed power transformer is an important parameter. It has a great influence on the transformer insulation aging and a close relationship with the transformer top oil temperature. The changes of non-linear thermal resistance, open-circuit impedance, and oil viscosity and winding losses with temperature are also taken into account. An improved model added on the transformer top oil temperature to calculate transformer winding hot-spot temperature by using the viscosity and loss correction factors is proposed. Model parameters are estimated by Levenberg-Marquardt method. In the end, by comparing with the measured data tested under different conditions, the model shows good consistency under underload (90%), rated load (100%) and overload (110%) conditions respectively, and describes the temperature variation more accurately in the dynamic loading profiles.

  8. Breakdown of Fermi liquid behavior near the hot spots in a two-dimensional model: A two-loop renormalization group analysis

    Energy Technology Data Exchange (ETDEWEB)

    Carvalho, Vanuildo S. de [Instituto de Física, Universidade Federal de Goiás, 74.001-970 Goiânia, GO (Brazil); Freire, Hermann, E-mail: hfreire@mit.edu [Instituto de Física, Universidade Federal de Goiás, 74.001-970 Goiânia, GO (Brazil); Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States)

    2013-10-21

    Motivated by a recent experimental observation of a nodal liquid on both single crystals and thin films of Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+} {sub δ} by Chatterjee et al. [Nature Phys. 6 (2010) 99], we perform a field-theoretical renormalization group (RG) analysis of a two-dimensional model such that only eight points located near the “hot spots” on the Fermi surface are retained, which are directly connected by spin density wave ordering wavevector. We derive RG equations up to two-loop order describing the flow of renormalized couplings, quasiparticle weight, several order-parameter response functions, and uniform spin and charge susceptibilities of the model. We find that while the order-parameter susceptibilities investigated here become non-divergent at two loops, the quasiparticle weight vanishes in the low-energy limit, indicating a breakdown of Fermi liquid behavior at this RG level. Moreover, both uniform spin and charge susceptibilities become suppressed in the scaling limit which indicate gap openings in both spin and charge excitation spectra of the model.

  9. Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats

    NARCIS (Netherlands)

    Dealtry, Simone; Ding, Guo-Chun; Weichelt, Viola; Dunon, Vincent; Schlüter, Andreas; Martini, María Carla; Del Papa, María Florencia; Lagares, Antonio; Amos, Gregory Charles Auton; Wellington, Elizabeth Margaret Helen; Gaze, William Hugo; Sipkema, Detmer; Sjöling, Sara; Springael, Dirk; Heuer, Holger; van Elsas, Jan Dirk; Thomas, Christopher; Smalla, Kornelia

    2014-01-01

    IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the

  10. Natural hot spots for gain of multiple resistances: arsenic and antibiotic resistances in heterotrophic, aerobic bacteria from marine hydrothermal vent fields.

    Science.gov (United States)

    Farias, Pedro; Espírito Santo, Christophe; Branco, Rita; Francisco, Romeu; Santos, Susana; Hansen, Lars; Sorensen, Soren; Morais, Paula V

    2015-04-01

    Microorganisms are responsible for multiple antibiotic resistances that have been associated with resistance/tolerance to heavy metals, with consequences to public health. Many genes conferring these resistances are located on mobile genetic elements, easily exchanged among phylogenetically distant bacteria. The objective of the present work was to isolate arsenic-, antimonite-, and antibiotic-resistant strains and to determine the existence of plasmids harboring antibiotic/arsenic/antimonite resistance traits in phenotypically resistant strains, in a nonanthropogenically impacted environment. The hydrothermal Lucky Strike field in the Azores archipelago (North Atlantic, between 11°N and 38°N), at the Mid-Atlantic Ridge, protected under the OSPAR Convention, was sampled as a metal-rich pristine environment. A total of 35 strains from 8 different species were isolated in the presence of arsenate, arsenite, and antimonite. ACR3 and arsB genes were amplified from the sediment's total DNA, and 4 isolates also carried ACR3 genes. Phenotypic multiple resistances were found in all strains, and 7 strains had recoverable plasmids. Purified plasmids were sequenced by Illumina and assembled by EDENA V3, and contig annotation was performed using the "Rapid Annotation using the Subsystems Technology" server. Determinants of resistance to copper, zinc, cadmium, cobalt, and chromium as well as to the antibiotics β-lactams and fluoroquinolones were found in the 3 sequenced plasmids. Genes coding for heavy metal resistance and antibiotic resistance in the same mobile element were found, suggesting the possibility of horizontal gene transfer and distribution of theses resistances in the bacterial population.

  11. Large-scale codon de-optimisation of the p29 replicase gene by synonymous substitutions causes a loss of infectivity of melon necrotic spot virus.

    Science.gov (United States)

    Usami, Atsushi; Mochizuki, Tomofumi; Tsuda, Shinya; Ohki, Satoshi T

    2013-09-01

    The effect of synonymous substitutions in the melon necrotic spot virus p29 replicase gene on viral pathogenicity was investigated. The codons in the p29 gene were replaced by the least frequently used synonymous codons in Arabidopsis thaliana or melons. Mechanical inoculation of melon with p29 variants resulted in a loss of viral infectivity when all, one-half, or one-quarter of the gene was de-optimised. The effect of the de-optimisation in one-sixth of the gene was different depending on the de-optimised region. These results demonstrate that large-scale codon bias de-optimisation without amino acid substitutions of the p29 gene alter viral infectivity.

  12. Sequencing, de novo assembly and characterization of the spotted scat Scatophagus argus (Linnaeus 1766) transcriptome for discovery of reproduction related genes and SSRs

    Science.gov (United States)

    Yang, Wei; Chen, Huapu; Cui, Xuefan; Zhang, Kewei; Jiang, Dongneng; Deng, Siping; Zhu, Chunhua; Li, Guangli

    2017-09-01

    Spotted scat (Scatophagus argus) is an economically important farmed fish, particularly in East and Southeast Asia. Because there has been little research on reproductive development and regulation in this species, the lack of a mature artificial reproduction technology remains a barrier for the sustainable development of the aquaculture industry. More genetic and genomic background knowledge is urgently needed for an in-depth understanding of the molecular mechanism of reproductive process and identification of functional genes related to sexual differentiation, gonad maturation and gametogenesis. For these reasons, we performed transcriptomic analysis on spotted scat using a multiple tissue sample mixing strategy. The Illumina RNA sequencing generated 118 510 486 raw reads. After trimming, de novo assembly was performed and yielded 99 888 unigenes with an average length of 905.75 bp. A total of 45 015 unigenes were successfully annotated to the Nr, Swiss-Prot, KOG and KEGG databases. Additionally, 23 783 and 27 183 annotated unigenes were assigned to 56 Gene Ontology (GO) functional groups and 228 KEGG pathways, respectively. Subsequently, 2 474 transcripts associated with reproduction were selected using GO term and KEGG pathway assignments, and a number of reproduction-related genes involved in sex differentiation, gonad development and gametogenesis were identified. Furthermore, 22 279 simple sequence repeat (SSR) loci were discovered and characterized. The comprehensive transcript dataset described here greatly increases the genetic information available for spotted scat and contributes valuable sequence resources for functional gene mining and analysis. Candidate transcripts involved in reproduction would make good starting points for future studies on reproductive mechanisms, and the putative sex differentiation-related genes will be helpful for sex-determining gene identification and sex-specific marker isolation. Lastly, the SSRs can serve as marker

  13. Characterization of the inaA gene and expression of ice nucleation phenotype in Pantoea ananatis isolates from Maize White Spot disease.

    Science.gov (United States)

    Miller, A M; Figueiredo, J E F; Linde, G A; Colauto, N B; Paccola-Meirelles, L D

    2016-03-04

    Maize White Spot (MWS), a foliar disease caused by Pantoea ananatis, could cause up to 60% yield loss. Some strains of P. ananatis harboring the ice nucleation gene inaA catalyze the formation of ice nuclei, causing tissue damage at temperatures slightly below freezing. Little is known about the relationship between the presence of the ina gene in this maize pathogen and its expression during the phenomenon of ice nucleus formation. Here, we attempted to verify the presence of the inaA gene and the expression of phenotype in vitro. The identity of the isolates and the presence of the inaA gene were determined by P. ananatis species-specific primers. The expression of the inaA gene was assessed in vitro by the visualization of ice-crystal formation in water at subzero temperatures. A total of ninety P. ananatis isolates from MWS lesions were characterized. The presence of the inaA gene was confirmed by gel electrophoresis of the 350-400-bp PCR products. The inaA primers did not lead to DNA fragment amplification in three isolates. The ice nucleation phenotype was expressed in 83.34% of the isolates carrying the inaA gene. Our study showed that the ice nucleation in P. ananatis isolated from MWS lesions was dependent on the presence of a functional ina gene in the genome. We also found evidence indicating that some P. ananatis strains have a mutated form of the inaA gene, producing a non-functional ice nucleation protein. This is the first report on inaA gene characterization in P. ananatis isolates from Maize White Spot.

  14. Spotted inflation

    Energy Technology Data Exchange (ETDEWEB)

    Matsuda, Tomohiro, E-mail: matsuda@sit.ac.jp [Laboratory of Physics, Saitama Institute of Technology, Fusaiji, Okabe-machi, Saitama 369-0293 (Japan)

    2010-11-01

    We describe new scenarios for generating curvature perturbations when inflaton (curvaton) has significant interactions. We consider a ''spot'', which arises from interactions associated with an enhanced symmetric point (ESP) on the trajectory. Our first example uses the spot to induce a gap in the field equation. We observe that the gap in the field equation may cause generation of curvature perturbation if it does not appear simultaneous in space. The mechanism is similar to the scenario of inhomogeneous phase transition. Then we observe that the spot interactions may initiate warm inflation in the cold Universe. Creation of cosmological perturbation is discussed in relation to the inflaton dynamics and the modulation associated with the spot interactions.

  15. Diversity of Archaea in Icelandic hot springs based on 16S rRNA and chaperonin genes.

    Science.gov (United States)

    Mirete, Salvador; de Figueras, Carolina G; González-Pastor, Jose E

    2011-07-01

    The diversity of archaeal communities growing in four hot springs (65-90 °C, pH 6.5) was assessed with 16S rRNA gene primers specific for the domain Archaea. Overall, mainly uncultured members of the Desulfurococcales, the Thermoproteales and the Korarchaeota, were identified. Based on this diversity, a set of chaperonin heat-shock protein (Hsp60) gene sequences from different archaeal species were aligned to design two degenerate primer sets for the amplification of the chaperonin gene: Ths and Kor (which can also detect the korarchaeotal chaperonin gene from one of the samples). A phylogenetic tree was constructed using the chaperonin sequences retrieved and other sequences from cultured representatives. The Alpha and Beta paralogs of the chaperonin gene were observed within the main clades and orthologs among them. Cultivated representatives from these clades were assigned to either paralog in the chaperonin tree. Uncultured representatives observed in the 16S rRNA gene analysis were found to be related to the Desulfurococcales. The topologies of the 16S rRNA gene and chaperonin phylogenetic trees were compared, and similar phylogenetic relationships were observed. Our results suggest that the chaperonin Hsp60 gene may be used as a phylogenetic marker for the clades found in this extreme environment.

  16. Molecular cloning of the insulin-like growth factor 3 and difference in the expression of igf genes in orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Yang, Huirong; Chen, Huapu; Zhao, Huihong; Liu, Li; Xie, Zhenzhen; Xiao, Ling; Li, Shuisheng; Zhang, Yong; Lin, Haoran

    2015-08-01

    Insulin-like growth factor (Igf) is the key regulator for development, growth, and reproduction. In most vertebrate species, the Igf family has two forms: Igf1 and Igf2. A novel form of Igf, termed Igf3, was recently discovered in fish. In the present study, we isolated igf3 from the orange-spotted grouper (Epinephelus coioides). The orange-spotted grouper igf3 consisted of a full-length cDNA of 1014 nucleotides with an open reading frame (ORF) of 597 bp, encoding for proteins of 199 amino acid residues in length. Tissue distribution analysis showed that igf1 widely expressed with the highest expression in the pituitary and liver. igf2 was expressed highly in all the tissues except the olfactory bulb, while igf3 showed the highest expression in the ovary, and moderate expression in brain areas. The expression profiles of three igf genes during the ovarian development and growth hormone (Gh) and human chorionic gonadotropin (hCG) treatment were also investigated. Three igf genes exhibited different expression patterns during the ovarian development, and showed different responses to the Gh and hCG treatments, appearing to play distinct roles in ovarian development. The present study provides further evidence for the existence of an intraovarian Igf system in orange-spotted grouper.

  17. Tracing back seed and pollen flow within the crop-wild Beta vulgaris complex: genetic distinctiveness vs. hot spots of hybridization over a regional scale.

    Science.gov (United States)

    Viard, Frédérique; Arnaud, Jean-François; Delescluse, Maxime; Cuguen, Joël

    2004-06-01

    Hybrids between transgenic crops and wild relatives have been documented successfully in a wide range of cultivated species, having implications on conservation and biosafety management. Nonetheless, the magnitude and frequency of hybridization in the wild is still an open question, in particular when considering several populations at the landscape level. The Beta vulgaris complex provides an excellent biological model to tackle this issue. Weed beets contaminating sugar beet fields are expected to act as a relay between wild populations and crops and from crops-to-crops. In one major European sugar beet production area, nine wild populations and 12 weed populations were genetically characterized using cytoplasmic markers specific to the cultivated lines and nuclear microsatellite loci. A tremendous overall genetic differentiation between neighbouring wild and weed populations was depicted. However, genetic admixture analyses at the individual level revealed clear evidence for gene flow between wild and weed populations. In particular, one wild population displayed a high magnitude of nuclear genetic admixture, reinforced by direct seed flow as evidenced by cytoplasmic markers. Altogether, weed beets were shown to act as relay for gene flow between crops to wild populations and crops to crops by pollen and seeds at a landscape level.

  18. Regional Fluctuation in the Functional Consequence of LINE-1 Insertion in the Mitf Gene: The Black Spotting Phenotype Arisen from the Mitfmi-bw Mouse Lacking Melanocytes.

    Directory of Open Access Journals (Sweden)

    Kazuhisa Takeda

    Full Text Available Microphthalmia-associated transcription factor (Mitf is a key regulator for differentiation of melanoblasts, precursors to melanocytes. The mouse homozygous for the black-eyed white (Mitfmi-bw allele is characterized by the white-coat color and deafness with black eyes due to the lack of melanocytes. The Mitfmi-bw allele carries LINE-1, a retrotransposable element, which results in the Mitf deficiency. Here, we have established the black spotting mouse that was spontaneously arisen from the homozygous Mitfmi-bw mouse lacking melanocytes. The black spotting mouse shows multiple black patches on the white coat, with age-related graying. Importantly, each black patch also contains hair follicles lacking melanocytes, whereas the white-coat area completely lacks melanocytes. RT-PCR analyses of the pigmented patches confirmed that the LINE-1 insertion is retained in the Mitf gene of the black spotting mouse, thereby excluding the possibility of the somatic reversion of the Mitfmi-bw allele. The immunohistochemical analysis revealed that the staining intensity for beta-catenin was noticeably lower in hair follicles lacking melanocytes of the homozygous Mitfmi-bw mouse and the black spotting mouse, compared to the control mouse. In contrast, the staining intensity for beta-catenin and cyclin D1 was higher in keratinocytes of the black spotting mouse, compared to keratinocytes of the control mouse and the Mitfmi-bw mouse. Moreover, the keratinocyte layer appears thicker in the Mitfmi-bw mouse, with the overexpression of Ki-67, a marker for cell proliferation. We also show that the presumptive black spots are formed by embryonic day 15.5. Thus, the black spotting mouse provides the unique model to explore the molecular basis for the survival and death of developing melanoblasts and melanocyte stem cells in the epidermis. These results indicate that follicular melanocytes are responsible for maintaining the epidermal homeostasis; namely, the present study

  19. Interaction of alleles of the relA, relC and spoT genes in Escherichia coli: analysis of the interconversion of GTP, ppGpp and pppGpp

    DEFF Research Database (Denmark)

    Fiil, N P; Willumsen, B M; Friesen, J D;

    1977-01-01

    Mutants in the spo T gene have been isolated as stringent second site revertants of the relC mutation. These show varying degrees of the characteristics associated with the spoT1 gene, viz relative amount and absolute levels of both pppGpp and ppGpp and the decay rate of the latter. The entry of 3H...... various conditions in various spoT+ and spoT- strains indicate that the ppGpp concentration indirectly controls the rate of pppGpp synthesis....

  20. Molecular cloning, characterization and expression profiles of multiple leptin genes and a leptin receptor gene in orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Zhang, Huixian; Chen, Huapu; Zhang, Yong; Li, Shuisheng; Lu, Danqi; Zhang, Haifa; Meng, Zining; Liu, Xiaochun; Lin, Haoran

    2013-01-15

    Leptin plays key roles in body weight regulation, energy metabolism, food intake, reproduction and immunity in mammals. However, its function in teleosts is still unclear. In the present study, two leptin genes (gLepA and gLepB) and one leptin receptor gene (gLepR) were cloned and characterized in orange-spotted grouper (Epinephelus coioides). The cDNAs of gLepA and gLepB were 671 bp and 684 bp in length, encoding for proteins of 161 amino acid (aa) and 158 aa, respectively. The three-dimensional (3D) structures modeling of gLepA and gLepB showed strong conservation of tertiary structure with that of other vertebrates. The total length of gLepR cDNA was 4242 bp, encoding a protein of 1169 aa which contained all functionally important domains conserved among vertebrate LEPR. Tissue distribution analysis showed that gLepA was highly expressed in cerebellum, liver and ovary, while gLepB mRNA abundantly in the brain regions, as well as in the ovary with some extend. The gLepR was mainly expressed in kidney, head kidney and most of brain regions. Analysis of expression profiles of gLep and gLepR genes during the embryonic stages showed that high expression of gLepR was observed in the brain vesicle stage, while neither gLepA nor gLepB mRNA was detected during different embryonic stages. Finally, fasting and refeeding experiments were carried out to investigate the possible function of leptin genes in food intake and energy metabolism, and the results showed that a significant increase of gLepA expression in the liver was induced by food deprivation in both short-term (7 days) and long-term (3 weeks) fasting and gLepA mRNA upregulation was eliminated after refeeding, while gLepB wasn't detected in the liver of grouper during fasting. No significant differences in hypothalamic leptin and leptin receptor expression were found during short-term fasting and refeeding. Hepatic expression of gLepA mRNA increased significantly 9h after a single meal. These results suggested g

  1. Characterizing seasonal variability of storm events based on very high frequency monitoring of hydrological and chemical variables: comparing patterns in hot spots and hot moments for nutrient and sediment export

    Science.gov (United States)

    Fovet, Ophelie; Thelusma, Gilbert; Humbert, Guillaume; Dupas, Rémi; Faucheux, Mikael; Gilliet, Nicolas; Hamon, Yannick; Jaffrezic, Anne; Grimaldi, Catherine; Gruau, Gerard

    2016-04-01

    Storm events are critical hot moments of emission for several dissolved and particulate chemical species at major stake for water quality (e.g. dissolved organic carbon DOC, suspended sediments, phosphorus). During such events, the solutes or particles are exported from heterogeneous sources through various pathways to stream leading to specific integrated signals at the outlet characterized by very short dynamics. This is merely true in headwater catchments where the total duration of such events ranges over 10h to 3 days, with very quick variations in stream flow and concentrations at the outlet occurring in a few hours. Thus for investigating properly event processes, high frequency monitoring of flow and water quality is required. We analysed 103 storm events in a 5 km2 agricultural headwater catchment, part of the AgrHys Observatory, on the basis of a 3-year-long data set which combined meterological (Rainfall), hydrological (flow and piezometry), and water quality (turbidity, conductivity, DOC and NO3 concentrations) data recorded at very high frequencies (from 1 to 20 min) thanks to dedicated sensors. We proposed a range of quantitative storm descriptors for characterizing input (rainfall), antecedent and initial conditions (groundwater levels and saturated area), and stream response in terms of level and dynamics of flow (Q), groundwater levels, and concentrations (C) but also the C-Q relationships. Three intra annual periods have been previously defined for base flow dynamic according to shallow groundwater table variations so that they correspond to different connectivity status in the catchment. The seasonal and inter-annual variability of the storm events have been analysed using the descriptors and based on these predefined periods. Results show that the hydrological flowpaths and the consequent storm chemistry were controlled by the hydrological base flow regime rather than by the rain input characteristics. This highlights that the exports of NO3

  2. Fine mapping and analysis of a candidate gene in tomato accession PI128216 conferring hypersensitive resistance to bacterial spot race T3.

    Science.gov (United States)

    Pei, Chengcheng; Wang, Hui; Zhang, Jieyun; Wang, Yuanyuan; Francis, David M; Yang, Wencai

    2012-02-01

    Bacterial spot caused by Xanthomonas euvesicatoria, X. vesicatoria, X. perforans and X. gardneri is one of the most destructive diseases in tomatoes (Solanum lycopersicum L.) growing in tropical and subtropical regions. Exploring resistance genes from diverse germplasm and incorporating them into cultivated varieties are critical for controlling this disease. The S. pimpinellifolium accession PI128216 was reported to carry the Rx4 gene on chromosome 11 conferring hypersensitivity and field resistance to race T3. To facilitate the use of marker-assisted selection in breeding and map-based cloning of the gene, an F(2) population derived from a cross between the susceptible variety OH88119 and the resistant accession PI128216 was created for fine mapping of the Rx4 gene. Using 18 markers developed through various approaches, we mapped the gene to a 45.1-kb region between two markers pcc17 and pcc14 on chromosome 11. A NBS-LRR class of resistance gene was identified as the candidate for the Rx4 gene based on annotation results from the International Tomato Annotation Group. Comparison of the genomic DNA sequences of the Rx4 alleles in PI128216 and OH88119 revealed a 6-bp insertion/deletion (InDel) and eight SNPs. The InDel marker was successfully used to distinguish resistance and susceptibility in 12 tomato lines. These results will facilitate cloning the Rx4 gene and provide a useful tool for marker-assisted selection of this gene in tomato breeding programs.

  3. Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Shi, Yan; Zhao, Zhe; Zhu, Xinping; Chen, Kunci; Zhang, Qiya

    2013-01-01

    GRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-β- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish.

  4. Spatial distributions of sulphur species and sulphate-reducing bacteria provide insights into sulphur redox cycling and biodegradation hot-spots in a hydrocarbon-contaminated aquifer

    Science.gov (United States)

    Einsiedl, Florian; Pilloni, Giovanni; Ruth-Anneser, Bettina; Lueders, Tillman; Griebler, Christian

    2015-05-01

    Dissimilatory sulphate reduction (DSR) has been proven to be one of the most relevant redox reactions in the biodegradation of contaminants in groundwater. However, the possible role of sulphur species of intermediate oxidation state, as well as the role of potential re-oxidative sulphur cycling in biodegradation particularly at the groundwater table are still poorly understood. Here we used a combination of stable isotope measurements of SO42-, H2S, and S0 as well as geochemical profiling of sulphur intermediates with special emphasis on SO32-, S2O32-, and S0 to unravel possible sulphur cycling in the biodegradation of aromatics in a hydrocarbon-contaminated porous aquifer. By linking these results to the quantification of total bacterial rRNA genes and respiratory genes of sulphate reducers, as well as pyrotag sequencing of bacterial communities over depth, light is shed on possible key-organisms involved. Our results substantiate the role of DSR in biodegradation of hydrocarbons (mainly toluene) in the highly active plume fringes above and beneath the plume core. In both zones the concentration of sulphur intermediates (S0, SO32- and S2O32-) was almost twice that of other sampling-depths, indicating intense sulphur redox cycling. The dual isotopic fingerprint of oxygen and sulphur in dissolved sulpha