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Sample records for gen irs1 snp43

  1. Polimorfisme Gly972Arg Gen IRS-1 dan Cys981Tyr Gen PTPN1 sebagai Faktor Risiko pada Sindrom Metabolik dengan Riwayat Berat Bayi Lahir Rendah

    Directory of Open Access Journals (Sweden)

    Hikmat Permana

    2012-09-01

    Full Text Available Increasing prevalence of obesity occurs worldwide and can affect all ages levels. Risk factors of increasing metabolic syndrome were associated with changes in lifestyle, obesity and low birth weight (LBW. This study was aimed to analyze the genotype IRS-1, genotype PTPN1 and LBW in metabolic syndrome risk factors. In 2009, this restrospective cohort study was comprised of 97 LBW and 100 normal birth weight (NBW, aged 20–21 years old, from the previous cohort study in District Tanjung Sari since 1989. The role of IRS-1 gene, PTPN1 gene and LBW in the metabolic syndrome factors were analyzed using multiple logistic regressions. The result showed there were no significant relationship between IRS-1 and PTPN1 genotype with metabolic syndrome factors but only IRS-1 genotype was significantly associated with trygliceride (p=0.006. Gly972Arg IRS-1 gene polymorphism in LBW has predictive value of increasing diastolic blood pressure, waist circumference and trygliceride. Cys981 Tyr PTPN1 gene polymorphism showed a risk factor for systolic blood pressure and high density lipoprotein (HDL cholesterol. Gly972Arg IRS-1 gene polymorphism was a dominant factor to increase diastolic blood pressure, waist circumference and trygliceride. In conclusions, Cys981 Tyr PTPN1 gene polymorphism is a risk factor of increased systolic blood pressure and HDL cholesterol, and LBW is the risk factor increasing fasting glucose level.

  2. Variantes polimórficas Ala513Pro y Gly972Arg del gen IRS-1 no se asocian a la diabetes mellitus tipo 2 en un grupo de la población cubana The Ala513Pro and Gly972ARg polymorphous variants of IRS-1 gen are not associated with type diabetes mellitus in a group of the Cuban population

    Directory of Open Access Journals (Sweden)

    Luis Miguel Pérez

    2011-08-01

    Full Text Available Introducción: la diabetes mellitus tipo 2 es una enfermedad heterogénea y multifactorial, que está determinada por factores genéticos y no genéticos. El sustrato 1 del receptor de la insulina (IRS-1 cumple una función fundamental en la transmisión de la señal insulínica, por tanto sus variantes génicas constituyen blancos importantes en el estudio de la susceptibilidad genética a esta enfermedad en las diferentes poblaciones. Objetivo: explorar el papel de las variantes polimórficas Gly972Arg y Ala513Pro del gen IRS-1 en la susceptibilidad genética de la diabetes mellitus tipo 2 en un grupo de la población cubana. Métodos: se determinó la frecuencia de los polimorfismos Gly972Arg y Ala513Pro del IRS-1 en 499 ciudadanos cubanos, con un índice de masa corporal entre 22-30, con edades comprendidas entre los 40 y 70 años: de ellos 272 (54,5 % diabéticos y 227 (45,5 % no diabéticos. Resultados: la frecuencia del alelo Pro513 fue baja (1,2 % y similar para ambos grupos (1,1 % vs. 1,3 % para el grupo de diabéticos y el grupo control, respectivamente. La frecuencia del polimorfismo Gly972Arg fue de 16,2%, superior a la reportada para la mayoría de las poblaciones estudiadas. No se encontraron diferencias significativas en la frecuencia del alelo Arg972 entre el grupo de diabéticos y el grupo control (15,4 % vs. 17,3 %, ni cambios en los niveles de glucemia e insulinemia asociados a la presencia del alelo polimórfico Arg972. Conclusiones: en este grupo de sujetos de la población cubana, las variantes polimórficas Ala513Pro y Gly972Arg del gen IRS-1 no participan en la etiología de la diabetes mellitus tipo 2.Introduction: the type 2 diabetes mellitus is a heterogeneous and multifactor disease determined by genetic and no-genetic factors. The substrate 1 of insulin receptor (IRS-1 has a fundamental function in transmission of insulin signal, thus its genic variants are significant targets in study of genetic susceptibility to

  3. BÚSQUEDA DE LA MUTACIÓN Phe-809 EN EL GEN DEL SUSTRATO DEL RECEPTOR DE INSULINA -1 (IRS-1 EN RATAS DIABÉTICAS eSS

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    Stella Maris Daniele

    2010-01-01

    Full Text Available El gen del sustrato del receptor de insulina IRS-1 juega un rol clave en la transducción de señales de insulina en músculo esquelético. Diversos polimorfismos del gen IRS-1 han sido reportados en estados de insulinorresistencia como obesidad y diabetes tipo 2.Las ratas eSS desarrollan espontáneamente diabetes tipo 2 magra, con insulinoresistencia caracterizada por hiperglucemia e hiperinsulinemia. Durante el segundo año existe disminución progresiva de la insulinemia y agravamiento del síndrome. Nuestro propósito fue buscar la mutación Phe-809 en el gen IRS-1, que implica sustitución del aminoácido Ser por Phe en el codon 809; dicho sitio se halla conservado en la rata. Esta variante fue identificada en pacientes diabéticos tipo 2. El gen del IRS-1 consta de un solo exón.Se analizó un fragmento de 281pb del gen IRS-1, que posee 90% de homología con el mismo fragmento en humanos. Se purificó ADN de leucocitos de un pool de sangre de ratas machos eSS y controles Wistar. Se cuantificó y amplificó por PCR utilizando un par de primers para amplificar ese mismo fragmento en el gen IRS-1 en humanos. Se usó sangre humana como control de amplificación. Se empleó el método de Sanger en la secuenciación del fragmento obtenido por PCR, previa extracción del mismo de un gel de agarosa al 2%. Se observó que la rata eSS y las controles mantuvieron la lectura esperada para ese fragmento, no encontrándose la mutación investigada. Se descarta la mutación Phe-809 como origen de la insulinoresistencia de la rata eSS.

  4. The IRS-1 signaling system.

    Science.gov (United States)

    Myers, M G; Sun, X J; White, M F

    1994-07-01

    Insulin-receptor substrate 1 (IRS-1) is a principal substrate of the receptor tyrosine kinase for insulin and insulin-like growth factor 1, and a substrate for a tyrosine kinase activated by interleukin 4. IRS-1 undergoes multisite tyrosine phosphorylation and mediates downstream signals by 'docking' various proteins that contain Src homology 2 domains. IRS-1 appears to be a unique molecule; however, 4PS, a protein found mainly in hemopoietic cells, may represent another member of this family.

  5. The IRS-1 signaling system.

    Science.gov (United States)

    White, M F

    1994-02-01

    IRS-1 is a principal substrate of the insulin receptor tyrosine kinase. It undergoes multi-site tyrosine phosphorylation and mediates the insulin signal by associating with various signaling molecules containing Src homology 2 domains. Interleukin-4 also stimulates IRS-1 phosphorylation, and it is suspected that a few more growth factors or cytokines will be added to form a select group of receptors that utilize the IRS-1 signaling pathway. More IRS-1-like adapter molecules, such as 4PS (IRS-2), may remain to be found.

  6. The cell growth suppressor, mir-126, targets IRS-1.

    Science.gov (United States)

    Zhang, Jin; Du, Ying-ying; Lin, Yi-feng; Chen, Ya-ting; Yang, Lu; Wang, Hui-jun; Ma, Duan

    2008-12-05

    miRNAs are a family of approximately 22-nuleotide-long noncoding RNAs involved in the formation and progress of tumors. Since traditional methods for the detection of miRNAs expression have many disadvantages, we developed a simple method called polyA RT PCR. With this method, we detected a series of miRNAs and found that mir-126 is one of the miRNAs underexpressed in breast cancer cells. Flow cytometry analysis showed that mir-126 inhibited cell cycle progression from G1/G0 to S. Further studies revealed that mir-126 targeted IRS-1 at the translation level. Knocking down of IRS-1 suppresses cell growth in HEK293 and breast cancer cell MCF-7, which recapitulates the effects of mir-126. In conclusion, we developed a simple method for high-throughput screening of miRNAs and found that mir-126, a cell growth suppressor, targets IRS-1.

  7. NG7538 IRS1 N: modeling a circumstellar maser disk

    CERN Document Server

    Pestalozzi, M R; Conway, J; Booth, R

    2004-01-01

    We present an edge-on Keplerian disk model to explain the main component of the 12.2 and 6.7 GHz methanol maser emission detected toward NGC7538-IRS1 N. The brightness distribution and spectrum of the line of bright masers are successfully modeled with high amplification of background radio continuum emission along velocity coherent paths through a maser disk. The bend seen in the position-velocity diagram is a characteristic signature of differentially rotating disks. For a central mass of 30 solar masses, suggested by other observations, our model fixes the masing disk to have inner and outer radii of about 270 AU and 750 AU.

  8. Impaired Insulin Signaling is Associated with Hepatic Mitochondrial Dysfunction in IR+/−-IRS-1+/− Double Heterozygous (IR-IRS1dh Mice

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    Andras Franko

    2017-05-01

    Full Text Available Mitochondria play a pivotal role in energy metabolism, but whether insulin signaling per se could regulate mitochondrial function has not been identified yet. To investigate whether mitochondrial function is regulated by insulin signaling, we analyzed muscle and liver of insulin receptor (IR+/−-insulin receptor substrate-1 (IRS-1+/− double heterozygous (IR-IRS1dh mice, a well described model for insulin resistance. IR-IRS1dh mice were studied at the age of 6 and 12 months and glucose metabolism was determined by glucose and insulin tolerance tests. Mitochondrial enzyme activities, oxygen consumption, and membrane potential were assessed using spectrophotometric, respirometric, and proton motive force analysis, respectively. IR-IRS1dh mice showed elevated serum insulin levels. Hepatic mitochondrial oxygen consumption was reduced in IR-IRS1dh animals at 12 months of age. Furthermore, 6-month-old IR-IRS1dh mice demonstrated enhanced mitochondrial respiration in skeletal muscle, but a tendency of impaired glucose tolerance. On the other hand, 12-month-old IR-IRS1dh mice showed improved glucose tolerance, but normal muscle mitochondrial function. Our data revealed that deficiency in IR/IRS-1 resulted in normal or even elevated skeletal muscle, but impaired hepatic mitochondrial function, suggesting a direct cross-talk between insulin signaling and mitochondria in the liver.

  9. Lack of Arg972 polymorphism in the IRS1 gene in Parakand Brazilian Indians

    OpenAIRE

    Bezerra, RMN; Chadid, TT; Altemani, CM; Sales, TSI; Menezes, R.; Soares, MCP; Saad, STO; Saad, MJA

    2004-01-01

    Several polymorphisms in the insulin receptor substrate-1 (IRS1) gene have been reported in the last years. The most common IRS1 variant, a Gly --> Arg substitution at codon 972 (Arg972 IRS1), is more prevalent among subjects who have features of insulin resistance syndrome associated, or not, with type 2 diabetes in European populations. To determine whether the absence of IRS1 polymorphism is a more general characteristic of Paleo-Indian-derived populations, we examined the Arg972 IRS1 poly...

  10. Lack of Arg972 polymorphism in the IRS1 gene in Parakanã Brazilian Indians.

    Science.gov (United States)

    Bezerra, Rosângela M N; Chadid, Thiago T; Altemani, Claúdia M; Sales, Teresa S I; Menezes, Raimundo; Soares, Manoel C P; Saad, Sara T O; Saad, Mario J A

    2004-02-01

    Several polymorphisms in the insulin receptor substrate-1 (IRS1) gene have been reported in the last years. The most common IRS1 variant, a Gly --> Arg substitution at codon 972 (Arg972 IRS1), is more prevalent among subjects who have features of insulin resistance syndrome associated, or not, with type 2 diabetes in European populations. To determine whether the absence of IRS1 polymorphism is a more general characteristic of Paleo-Indian-derived populations, we examined the Arg972 IRS1 polymorphism in Parakanã Indians and found a lack of this polymorphism in the Parakanã population.

  11. The insulin receptor substrate 1 (IRS1 in intestinal epithelial differentiation and in colorectal cancer.

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    Diana L Esposito

    Full Text Available Colorectal cancer (CRC is associated with lifestyle factors that affect insulin/IGF signaling, of which the insulin receptor substrate 1 (IRS1 is a key transducer. We investigated expression, localization and pathologic correlations of IRS1 in cancer-uninvolved colonic epithelium, primary CRCs with paired liver metastases and in vitro polarizing Caco2 and HT29 cells. IRS1 mRNA and protein resulted higher, relative to paired mucosa, in adenomas of familial adenomatous polyposis patients and in CRCs that overexpressed c-MYC, ß-catenin, InsRß, and IGF1R. Analysis of IRS1 immunostaining in 24 cases of primary CRC with paired colonic epithelium and hepatic metastasis showed that staining intensity was significantly higher in metastases relative to both primary CRC (P<0.01 and colonic epithelium (P<0.01. Primary and metastatic CRCs, compared to colonic epithelium, contained significantly higher numbers of IRS1-positive cells (P = 0.013 and P = 0.014, respectively. Pathologic correlations in 163 primary CRCs revealed that diffuse IRS1 staining was associated with tumors combining differentiated phenotype and aggressive markers (high Ki67, p53, and ß-catenin. In Caco 2 IRS1 and InsR were maximally expressed after polarization, while IGF1R was highest in pre-polarized cells. No nuclear IRS1 was detected, while, with polarization, phosphorylated IRS1 (pIRS1 shifted from the lateral to the apical plasma membrane and was expressed in surface cells only. In HT29, that carry mutations constitutively activating survival signaling, IRS1 and IGF1R decreased with polarization, while pIRS1 localized in nuclear spots throughout the course. Overall, these data provide evidence that IRS1 is modulated according to CRC differentiation, and support a role of IRS1 in CRC progression and liver metastatization.

  12. GEN 480 uop / uophelp

    OpenAIRE

    2015-01-01

    GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEM 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment Paper and Matrix GEN 480 Week 2 DQ 1 GEN 480 Week 2 DQ 2 GEN 480 Week 2 DQ 3 ...

  13. Insulin receptor substrate-1 (IRS-1 associates with small nucleolar RNA which contributes to ribosome biogenesis

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    Atsufumi eOzoe

    2014-03-01

    Full Text Available Insulin receptor substrates (IRSs are well known to play crucial roles in mediating intracellular signals of insulin-like growth factors (IGFs/insulin. Previously we showed that IRS-1 forms high molecular mass complexes containing RNAs. To identify RNAs in IRS-1 complexes, we performed UV cross-linking and immunoprecipitation (CLIP analysis using HEK293 cells expressing FLAG-IRS-1 and FLAG-IRS-2. We detected the radioactive signals in the immunoprecipitates of FLAG-IRS-1 proportional to the UV irradiation, but not in the immunoprecipitates of FLAG-IRS-2, suggesting the direct contact of RNAs with IRS-1. RNAs cross-linked to IRS-1 were then amplified by RT-PCR, followed by sequence analysis. We isolated sequence tags attributed to 25 messenger RNAs and 8 non-coding RNAs, including small nucleolar RNAs (snoRNAs. We focused on the interaction of IRS-1 with U96A snoRNA (U96A and its host Rack1 (receptor for activated C kinase 1 pre-mRNA. We confirmed the interaction of IRS-1 with U96A, and with RACK1 pre-mRNA by immunoprecipitation with IRS-1 followed by Northern blotting or RT-PCR analyses. Mature U96A in IRS-1-/- mouse embryonic fibroblasts was quantitatively less than WT. We also found that a part of nuclear IRS-1 is localized in the Cajal body, a nuclear subcompartment where snoRNA mature. The unanticipated function of IRS-1 in snoRNA biogenesis highlights the potential of RNA-associated IRS-1 complex to open a new line of investigation to dissect the novel mechanisms regulating IGFs/insulin-mediated biological events.

  14. GEN 300 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 300 Ethics in an Academic Environment Assignment POWERPOINT ONLY GEN 300 Team Dynamics Instructions GEN 300 Effects of Technology Essay GEN 300 Research,Summary, and Paraphrase Activity GEN 300 Ethics in an Academic Environment Assignment PAPER ONLY GEN 300 Final Paper on Team Dynamics GEN 300 Student Web Scavenger Hunt GEN 300 Week 1 DQs GEN 300 Week 2 DQs GEN 300 Week 3 DQs GEN 300 Week 4 DQs GEN 300 Week 5 DQ

  15. GEN 105 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 105 Assignment: Reading and Retention GEN 105 Assignment: Elevator Speech GEN 105 CheckPoint: Technological Tools GEN 105 CheckPoint: Distance Learning I GEN 105 CheckPoint: Distance Learning II GEN 105 CheckPoint: Communicating in Forums GEN 105 Week 2 Discussion Questions GEN 105 Week 4 Discussion Questions GEN 105 Week 6 Discussion Questions GEN 105 Week 8 Discussion Questions GEN 105 Assignment: University Library Article Search GEN 105 Chec...

  16. GEN 480 UOP Course Tutorial / gen480dotcom

    OpenAIRE

    2015-01-01

    GEN 480 Entire Course For more course tutorials visit www.gen480.com   GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEN 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment P...

  17. GEN 200 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 200 Week 1 Assignment- Map Out an Important Goal GEN 200 Week 1 DQ 1 GEN 200 Week 1 DQ 2 GEN 200 Week 2 Assignment- Communication and Collaboration Strategy Paper GEN 200 Week 2 DQ 1 GEN 200 Week 2 DQ 2 GEN 200 Week 3 Assignment- Student Web Scavenger Hunt GEN 200 Week 3 DQ 1 GEN 200 Week 3 DQ 2 GEN 200 Week 4 Assignment-Research Strategy Paper GEN 200 Week 4 DQ 1 GEN 200 Week 4 DQ 2 GEN 200 Week 5 DQ 1 GEN 200 Week 5 DQ 2 GEN 200 We...

  18. Cloning, chromosomal localization, SNP detection and association analysis of the porcine IRS-1 gene.

    Science.gov (United States)

    Niu, P-X; Huang, Z; Li, C-C; Fan, B; Li, K; Liu, B; Yu, M; Zhao, S-H

    2009-11-01

    Insulin receptor substrate-1(IRS-1) gene is one member of the Insulin receptor substrate (IRS) gene family, which plays an important role in mediating the growth of skeletal muscle and the molecular metabolism of type 2 diabetes. Here, we cloned a 3,573 bp fragment of the partial CDS sequence of porcine IRS-1 gene by in silicon cloning strategy and RT-PCR method. The porcine IRS-1 gene was assigned to SSC15q25 by using IMpRH. Sequencing of PCR products from Duroc and Tibetan pig breeds identified one SNP in exon 1 of porcine IRS-1 gene (C3257A polymorphisms). Association analysis of genotypes with the growth traits, anatomy traits, meat quality traits and physiological biochemical indexes traits showed that different genotypes at locus 3,257 of IRS-1 have significant differences in carcass straight length in pigs (P = 0.0102 \\ 0.05).

  19. TGFβ/Smad3 regulates proliferation and apoptosis through IRS-1 inhibition in colon cancer cells.

    Science.gov (United States)

    Bailey, Katie L; Agarwal, Ekta; Chowdhury, Sanjib; Luo, Jiangtao; Brattain, Michael G; Black, Jennifer D; Wang, Jing

    2017-01-01

    In this study, we have uncovered a novel crosstalk between TGFβ and IGF-1R signaling pathways. We show for the first time that expression and activation of IRS-1, an IGF-1R adaptor protein, is decreased by TGFβ/Smad3 signaling. Loss or attenuation of TGFβ activation leads to elevated expression and phosphorylation of IRS-1 in colon cancer cells, resulting in enhanced cell proliferation, decreased apoptosis and increased tumor growth in vitro and in vivo. Downregulation of IRS-1 expression reversed Smad3 knockdown-mediated oncogenic phenotypes, indicating that TGFβ/Smad3 signaling inhibits cell proliferation and increases apoptosis at least partially through the inhibition of IRS-1 expression and activation. Additionally, the TGFβ/Smad3/IRS-1 signaling axis regulates expression of cyclin D1 and XIAP, which may contribute to TGFβ/Smad3/IRS-1-mediated cell cycle progression and survival. Given that loss of TGFβ signaling occurs frequently in colon cancer, an important implication of our study is that IRS-1 could be a potential therapeutic target for colon cancer treatment.

  20. MicroRNA-145 suppresses hepatocellular carcinoma by targeting IRS1 and its downstream Akt signaling

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yelin [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Hu, Chen; Cheng, Jun [Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Chen, Binquan [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Ke, Qinghong; Lv, Zhen; Wu, Jian [Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China); Zhou, Yanfeng, E-mail: zyfhdj@yahoo.com [Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou (China)

    2014-04-18

    Highlights: • MiR-145 expression is down-regulated in HCC tissues and inversely related with IRS1 levels. • MiR-145 directly targets IRS1 in HCC cells. • Restored expression of miR-145 suppressed HCC cell proliferation and growth. • MiR-145 induced IRS1 under-expression potentially reduced downstream AKT signaling. - Abstract: Accumulating evidences have proved that dysregulation of microRNAs (miRNAs) is involved in cancer initiation and progression. In this study, we showed that miRNA-145 level was significantly decreased in hepatocellular cancer (HCC) tissues and cell lines, and its low expression was inversely associated with the abundance of insulin receptor substrate 1 (IRS1), a key mediator in oncogenic insulin-like growth factor (IGF) signaling. We verified IRS1 as a direct target of miR-145 using Western blotting and luciferase reporter assay. Further, the restoration of miR-145 in HCC cell lines suppressed cancer cell growth, owing to down-regulated IRS1 expression and its downstream Akt/FOXO1 signaling. Our results demonstrated that miR-145 could inhibit HCC through targeting IRS1 and its downstream signaling, implicating the loss of miR-145 regulation may be a potential molecular mechanism causing aberrant oncogenic signaling in HCC.

  1. Tissue-specific regulation of IRS-1 in unilaterally nephrectomized rats

    Directory of Open Access Journals (Sweden)

    Sasse A.D.

    1997-01-01

    Full Text Available Insulin stimulates the tyrosine kinase activity of its receptor, resulting in the phosphorylation of its cytosolic substrate, insulin receptor substrate 1 (IRS-1. IRS-1 is also a substrate for different peptides and growth factors, and a transgenic mouse "knockout" for this protein does not have normal growth. However, the role of IRS-1 in kidney hypertrophy and/or hyperplasia was not investigated. In the present study we investigated IRS-1 protein and tyrosine phosphorylation levels in the remnant kidney after unilateral nephrectomy (UNX in 6-week-old male Wistar rats. After insulin stimulation the levels of insulin receptor and IRS-1 tyrosine phosphorylation were reduced to 79 ± 5% (P<0.005 and 58 ± 6% (P<0.0001, respectively, of the control (C levels, in the remnant kidney. It is possible that a circulating factor and/or a local (paracrine factor playing a role in kidney growth can influence the early steps of insulin action in parallel. To investigate the hypothesis of a circulating factor, we studied the early steps of insulin action in liver and muscle of unilateral nephrectomized rats. There was no change in pp185 tyrosine phosphorylation levels in liver (C 100 ± 12% vs UNX 89 ± 9%, NS and muscle (C 100 ± 22% vs UNX 91 ± 17%, NS, and also there was no change in IRS-1 phosphorylation levels in both tissues. These data demonstrate that after unilateral nephrectomy there is a decrease in insulin-induced insulin receptor and IRS-1 tyrosine phosphorylation levels in kidney but not in liver and muscle. It will be of interest to investigate which factors, probably paracrine ones, regulate these early steps of insulin action in the contralateral kidney of unilaterally nephrectomized rats.

  2. GenBank

    Data.gov (United States)

    U.S. Department of Health & Human Services — GenBank is the NIH genetic sequence database, an annotated collection of all publicly available DNA sequences. GenBank is designed to provide and encourage access...

  3. GEN 499 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 499 Week 1 DQ 1 Final Research Paper Topic and Plan GEN 499 Week 1 DQ 2 Social Media GEN 499 Week 2 DQ 1 Professional Resume and Cover Letter GEN 499 Week 2 Assignment Critiquing Internet Sources GEN 499 Week 3 DQ 1 Social Capital GEN 499 Week 3 DQ 2 Federal Policy GEN 499 Week 3 Assignment Annotated Bibliography GEN 499 Week 4 DQ 1 Call to Action GEN 499 Week 4 DQ 2 Final Research Paper Progress GEN 499 Week 4 Critical Thinking Quiz GEN 499 Week 5 ...

  4. GEN 480 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEM 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment Paper and Matrix GEN 480 Week 2 DQ 1 GEN 480 Week 2 DQ 2 GEN 480 Week 2 DQ 3 ...

  5. GEN 480 UOP TUTORIAL / Uoptutorial

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    2015-01-01

    For more course tutorials visit www.uoptutorial.com           GEN 480 Week 1 DQ 1  GEN 480 Week 1 DQ 2  GEN 480 Week 1 DQ 3  GEN 480 Week 1 DQ 4  GEN 480 Week 1 Individual AssignmentEthics Awareness  GEN 480 Week 1 Summary  GEN 480 Week 2 DQ 1  GEN 480 Week 2 DQ 2  GEN 480 Week 2 DQ 3  GEN 480 Week 2 DQ 4  GEN 480 Week 2 Individual Assignment Ethics Awareness &...

  6. IRS-1 serine phosphorylation and insulin resistance in skeletal muscle from pancreas tranplant recipient

    DEFF Research Database (Denmark)

    Bouzakri, K; Karlsson, HRK; Vestergaard, Henrik

    2006-01-01

    Insulin-dependent diabetic recipients of successful pancreas allografts achieve self-regulatory insulin secretion and discontinue exogenous insulin therapy; however, chronic hyperinsulinemia and impaired insulin sensitivity generally develop. To determine whether insulin resistance is accompanied....... In conclusion, peripheral insulin resistance in pancreas-kidney transplant recipients may arise from a negative feedback regulation of the canonical insulin-signaling cascade from excessive serine phosphorylation of IRS-1, possibly as a consequence of immunosuppressive therapy and hyperinsulinemia....... insulin receptor substrate (IRS)-1 Ser (312) and Ser (616) phosphorylation, IRS-1-associated phosphatidylinositol 3-kinase activity, and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation were elevated in pancreas-kidney transplant recipients, coincident with fasting hyperinsulinemia. Basal...

  7. IRS-1 serine phosphorylation and insulin resistance in skeletal muscle from pancreas tranplant recipient

    DEFF Research Database (Denmark)

    Bouzakri, K; Karlsson, HRK; Vestergaard, Henrik

    2006-01-01

    Insulin-dependent diabetic recipients of successful pancreas allografts achieve self-regulatory insulin secretion and discontinue exogenous insulin therapy; however, chronic hyperinsulinemia and impaired insulin sensitivity generally develop. To determine whether insulin resistance is accompanied....... In conclusion, peripheral insulin resistance in pancreas-kidney transplant recipients may arise from a negative feedback regulation of the canonical insulin-signaling cascade from excessive serine phosphorylation of IRS-1, possibly as a consequence of immunosuppressive therapy and hyperinsulinemia....... insulin receptor substrate (IRS)-1 Ser (312) and Ser (616) phosphorylation, IRS-1-associated phosphatidylinositol 3-kinase activity, and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation were elevated in pancreas-kidney transplant recipients, coincident with fasting hyperinsulinemia. Basal...

  8. IRS-1: essential for insulin- and IL-4-stimulated mitogenesis in hematopoietic cells.

    Science.gov (United States)

    Wang, L M; Myers, M G; Sun, X J; Aaronson, S A; White, M; Pierce, J H

    1993-09-17

    Although several interleukin-3 (IL-3)-dependent cell lines proliferate in response to IL-4 or insulin, the 32D line does not. Insulin and IL-4 sensitivity was restored to 32D cells by expression of IRS-1, the principal substrate of the insulin receptor. Although 32D cells possessed receptors for both factors, they lacked the IRS-1--related protein, 4PS, which becomes phosphorylated by tyrosine in insulin- or IL-4--responsive lines after stimulation. These results indicate that factors that bind unrelated receptors can use similar mitogenic signaling pathways in hematopoietic cells and that 4PS and IRS-1 are functionally similar proteins that are essential for insulin- and IL-4--induced proliferation.

  9. IGF1 regulates RUNX1 expression via IRS1/2: Implications for antler chondrocyte differentiation.

    Science.gov (United States)

    Yang, Zhan-Qing; Zhang, Hong-Liang; Duan, Cui-Cui; Geng, Shuang; Wang, Kai; Yu, Hai-Fan; Yue, Zhan-Peng; Guo, Bin

    2017-03-19

    Although IGF1 is important for the proliferation and differentiation of chondrocytes, its underlying molecular mechanism is still unknown. Here we addressed the physiologic function of IGF1 in antler cartilage and explored the interplay of IGF1, IRS1/2 and RUNX1 in chondrocyte differentiation. The results showed that IGF1 was highly expressed in antler chondrocytes. Exogenous rIGF1 could increase the proliferation of chondrocytes and cell proportion in the S phase, whereas IGF1R inhibitor PQ401 abrogated the induction by rIGF1. Simultaneously, IGF1 could stimulate the expression of IHH which was a well-known marker for prehypertrophic chondrocytes. Further analysis evidenced that IGF1 regulated the expression of IRS1/2 whose silencing resulted in a rise of IHH mRNA levels, but the regulation was impeded by PQ401. Knockdown of IRS1 or IRS2 with specific siRNA could greatly enhance rIGF1-induced chondrocyte differentiation and reduce the expression of RUNX1. Extraneous rRUNX1 might rescue the effects of IRS1 or IRS2 siRNA on the differentiation. In antler chondrocytes, IGF1 played a role in modulating the expression of RUNX1 through IGF1R. Moreover, attenuation of RUNX1 expression advanced the differentiation elicited by rIGF1, while administration of rRUNX1 to chondrocytes treated with IGF1 siRNA or PQ401 reduced their differentiation. Additionally, siRNA-mediated downregulation of IRS1 or IRS2 in the chondrocytes impaired the interaction between IGF1 and RUNX1. Collectively, IGF1 could promote the proliferation and differentiation of antler chondrocytes. Furthermore, IRS1/2 might act downstream of IGF1 to regulate chondrocyte differentiation through targeting RUNX1.

  10. Relationship between the Mutation of IRS-1 Gene and β3-adrenergic Receptor Gene

    Institute of Scientific and Technical Information of China (English)

    丁国宪; 沈捷; 陈家伟

    2001-01-01

    Objective To explore the relationship between the mutation of Insulin receptor substrate-1 ( IRS-1) gene and β3-adrenergic receptor (β3-AR) gene associated with insulin resistance, to further elucidate the etiology and pathogenesis of type 2 DM, hypertension and coronary heart disease. Methods 281 Chinese subjects are divided into three groups according to the oral glucose tolerance test (OGTT), The subjects were genotyped for the codon 64 of β3-AR gene, the codon 972 of IRS-1 gene polymorphisms by applying polymerase chain reaction (PCR) restriction fragment-length polymorphisms (RFLP) screening. Results Our study found that there was significantly increased frequency of IRS-1 gene mutation in IGT subjects and type 2 DM patients (P<0.05, 0.01, respectively), increased frequency of β3-AR gene mutation in type 2 DM patients (P<0.01), compared with NGT subjects. After adjusted for age, sex and plasma glucose, the level of insulin was significantly correlated with polymorphism of IRS-1 gene and β3-AR gene (P<0.001 in all ) by multiple regression analysis. In the models of Logistic regression, type 2 DM is closely related to age and family history (OR=3.1966, 1.4670; P=0.0272, 0.009; respectively), and to the polymorphism of β3-AR gene (OR=1.7380, P=0.0356), but not related to the polymorphism of IRS-1 gene. Conclusions These results suggest that mutation of IRS-1 gene may be the risk factor for insulin resistance, whereas mutation of β3-AR gene may be a common risk factor for insulin resistance, obesity, type 2 DM and hypertension.

  11. Physiological and genomic characterization of Arcobacter anaerophilus IR-1 reveals new metabolic features in Epsilonproteobacteria

    Directory of Open Access Journals (Sweden)

    Irene eRoalkvam

    2015-09-01

    Full Text Available In this study we characterized and sequenced the genome of Arcobacter anaerophilus strain IR-1 isolated from enrichment cultures used in nitrate-amended corrosion experiments. A. anaerophilus IR-1 could grow lithoautotrophically on hydrogen and hydrogen sulfide and lithoheterothrophically on thiosulfate and elemental sulfur. In addition, the strain grew organoheterotrophically on yeast extract, peptone and various organic acids. We show for the first time that Arcobacter could grow on the complex organic substrate tryptone and oxidize acetate with elemental sulfur as electron acceptor. Electron acceptors utilized by most Epsilonproteobacteria, such as oxygen, nitrate and sulfur, were also used by A. anaerophilus IR-1. Strain IR-1 was also uniquely able to use iron citrate as electron acceptor. Comparative genomics of the Arcobacter strains A. butzleri RM4018, A. nitrofigilis CI and A. anaerophilus IR-1 revealed that the free-living strains had a wider metabolic range and more genes in common compared to the pathogen strain. The presence of genes for NAD+-reducing hydrogenase (hox and dissimilatory iron reduction (fre were unique for A. anaerophilus IR-1 among Epsilonproteobacteria. Finally, the new strain had an incomplete denitrification pathway where the end product was nitrite, which is different from other Arcobacter strains where the end product is ammonia. Altogether, our study shows that traditional characterization in combination with a modern genomics approach can expand our knowledge on free-living Arcobacter, and that this complementary approach could also provide invaluable knowledge about the physiology and metabolic pathways in other Epsilonproteobacteria from various environments.

  12. Preclinical effectiveness of selective inhibitor of IRS1/2 NT157 in osteosarcoma cell lines.

    Directory of Open Access Journals (Sweden)

    Cecilia eGarofalo

    2015-05-01

    Full Text Available Osteosarcoma (OS is the most common primary bone tumor in children and young adults. Several studies have confirmed the involvement of the IGF system in the regulation of OS cell proliferation and differentiation as well as in the protection of cells from chemotherapy. IRS-1 is a critical mediator of IGF-1R signaling, and we recently reported that its overexpression in OS cells increases proliferation, migration and metastasis both in vitro and in vivo. In this study, we evaluated the efficacy of NT157, a selective inhibitor of IRS-1/2, in a panel of OS cells. A strong dose-dependent inhibition of growth was observed in the MG-63, OS-19, and U-2OS OS cell lines, displaying IC50 values at sub-micromolar doses after 72 h of treatment. Exposure to NT157 elicited dose- and time- dependent decreases in IRS-1 levels. Moreover, a protein analysis showed that the degradation of IRS-1 inhibited the activation of principal downstream mediators of the IGF pathway. NT157 significantly affected the cells’ migratory ability, as confirmed by a wound-healing assay. The inhibitor induced cytostatic effects, as evidenced by G2/M cell cycle arrest, and did not affect apoptosis. Consequently, NT157 was combined with drugs used to treat OS in order to capitalize on its therapeutic potential. Simultaneous treatments were made in association with chemotherapeutic agents in a fixed ratio for 72 h and cell proliferation was determined by MTT assay. Synergistic or addictive effects with respect to single agents are expressed as the combination index (CI. Significant synergistic effects were obtained with several targeted drugs, such as Everolimus, an mTOR inhibitor, and NVP-BEZ235, a dual inhibitor of PI3K/mTOR.Overall, these findings provide evidence for the effectiveness of a selected inhibitor of IRS1/2 NT157 in OS cells, displaying a promising approach based on the targeting of IRS-1 combined with other therapies for the treatment of this pediatric solid tumor.

  13. Preclinical Effectiveness of Selective Inhibitor of IRS-1/2 NT157 in Osteosarcoma Cell Lines

    Science.gov (United States)

    Garofalo, Cecilia; Capristo, Mariantonietta; Mancarella, Caterina; Reunevi, Hadas; Picci, Piero; Scotlandi, Katia

    2015-01-01

    Osteosarcoma (OS) is the most common primary bone tumor in children and young adults. Several studies have confirmed the involvement of the insulin-like growth factor (IGF) system in the regulation of OS cell proliferation and differentiation as well as in the protection of cells from chemotherapy. Insulin receptor substrate (IRS)-1 is a critical mediator of IGF-1R signaling, and we recently reported that its overexpression in OS cells increases proliferation, migration, and metastasis both in vitro and in vivo. In this study, we evaluated the efficacy of NT157, a selective inhibitor of IRS-1/2, in a panel of OS cells. A strong dose-dependent inhibition of growth was observed in the MG-63, OS-19, and U-2OS OS cell lines, displaying IC50 values at sub-micromolar doses after 72 h of treatment. Exposure to NT157 elicited dose- and time-dependent decreases in IRS-1 levels. Moreover, a protein analysis showed that the degradation of IRS-1 inhibited the activation of principal downstream mediators of the IGF pathway. NT157 significantly affected the cells’ migratory ability, as confirmed by a wound-healing assay. The inhibitor induced cytostatic effects, as evidenced by G2/M cell cycle arrest, and did not affect apoptosis. Consequently, NT157 was combined with drugs used to treat OS in order to capitalize on its therapeutic potential. Simultaneous treatments were made in association with chemotherapeutic agents in a fixed ratio for 72 h and cell proliferation was determined by MTT assay. Synergistic or addictive effects with respect to single agents are expressed as the combination index. Significant synergistic effects were obtained with several targeted drugs, such as Everolimus, a mammalian target of rapamycin (mTOR) inhibitor, and NVP-BEZ235, a dual inhibitor of PI-3K/mTOR. Overall, these findings provide evidence for the effectiveness of a selected inhibitor of IRS-1/2 NT157 in OS cells, displaying a promising approach based on the targeting of IRS-1 combined

  14. IRS-1 serine phosphorylation and insulin resistance in skeletal muscle from pancreas transplant recipients

    DEFF Research Database (Denmark)

    Bouzakri, Karim; Karlsson, Håkan K R; Vestergaard, Henrik

    2006-01-01

    Insulin-dependent diabetic recipients of successful pancreas allografts achieve self-regulatory insulin secretion and discontinue exogenous insulin therapy; however, chronic hyperinsulinemia and impaired insulin sensitivity generally develop. To determine whether insulin resistance is accompanied...... by altered signal transduction, skeletal muscle biopsies were obtained from pancreas-kidney transplant recipients (n = 4), nondiabetic kidney transplant recipients (receiving the same immunosuppressive drugs; n = 5), and healthy subjects (n = 6) before and during a euglycemic-hyperinsulinemic clamp. Basal...... insulin receptor substrate (IRS)-1 Ser (312) and Ser (616) phosphorylation, IRS-1-associated phosphatidylinositol 3-kinase activity, and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation were elevated in pancreas-kidney transplant recipients, coincident with fasting hyperinsulinemia. Basal...

  15. IRS-1 serine phosphorylation and insulin resistance in skeletal muscle from pancreas tranplant recipient

    DEFF Research Database (Denmark)

    Bouzakri, K; Karlsson, HRK; Vestergaard, Henrik;

    2006-01-01

    Insulin-dependent diabetic recipients of successful pancreas allografts achieve self-regulatory insulin secretion and discontinue exogenous insulin therapy; however, chronic hyperinsulinemia and impaired insulin sensitivity generally develop. To determine whether insulin resistance is accompanied...... by altered signal transduction, skeletal muscle biopsies were obtained from pancreas-kidney transplant recipients (n = 4), nondiabetic kidney transplant recipients (receiving the same immunosuppressive drugs; n = 5), and healthy subjects (n = 6) before and during a euglycemic-hyperinsulinemic clamp. Basal...... insulin receptor substrate (IRS)-1 Ser (312) and Ser (616) phosphorylation, IRS-1-associated phosphatidylinositol 3-kinase activity, and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation were elevated in pancreas-kidney transplant recipients, coincident with fasting hyperinsulinemia. Basal...

  16. Yo-Yo IR1 vs. incremental continuous running test for prediction of 3000-m performance.

    Science.gov (United States)

    Schmitz, Boris; Klose, Andreas; Schelleckes, Katrin; Jekat, Charlotte M; Krüger, Michael; Brand, Stefan-Martin

    2017-11-01

    This study aimed to compare physiological responses during the Yo-Yo intermittent recovery level 1 (Yo-Yo IR1) Test and an incremental continuous running field Test (ICRT) and to analyze their predictive value on 3000-m running performance. Forty moderately trained individuals (18 females) performed the ICRT and Yo-Yo IR1 Test to exhaustion. The ICRT was performed as graded running test with an increase of 2.0 km·h-1 after each 3 min interval for lactate diagnostic. In both tests, blood lactate levels were determined after the test and at 2 and 5 min of recovery. Heart rate (HR) was recorded to monitor differences in HR slopes and HR recovery. Comparison revealed a correlation between ICRT and Yo-Yo IR1 Test performance (R2=0.83, Prunning time (R2=0.77, Prunning performances such as 3000-m runs but maximum HR and blood lactate values differ significantly. The ICRT may have higher predictive power for middle- to long- distance running performance such as 3000-m runs offering a reliable test for coaches in the recruitment of athletes or supervision of training concepts.

  17. Polycyclic aromatic hydrocarbon degradation by biosurfactant-producing Pseudomonas sp. IR1

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, M. [Unidad de Biotecnologia del Petroleo, Centro de Biotecnologia, Fundacion Inst. de Estudios Avanzados (IDEA), Caracas (Venezuela); Synthesis and Biotics Div., Indian Oil Corp., Research and Development Center, Haryana (India); Leon, V.; Materano, A.D.S.; Ilzins, O.A.; Galindo-Castro, I.; Fuenmayor, S.L. [Unidad de Biotecnologia del Petroleo, Centro de Biotecnologia, Fundacion Inst. de Estudios Avanzados (IDEA), Caracas (Venezuela)

    2006-03-15

    We characterized a newly isolated bacterium, designated as IR1, with respect to its ability to degrade polycyclic aromatic hydrocarbons (PAHs) and to produce biosurfactants. Isolated IR1 was identified as Pseudomonas putida by analysis of 16S rRNA sequences (99.6% homology). It was capable of utilizing two-, three- and four-ring PAHs but not hexadecane and octadecane as a sole carbon and energy source. PCR and DNA hybridization studies showed that enzymes involved in PAH metabolism were related to the naphthalene dioxygenase pathway. Observation of both tensio-active and emulsifying activities indicated that biosurfactants were produced by IR1 during growth on both water miscible and immiscible substrates. The biosurfactants lowered the surface tension of medium from 54.9 dN cm{sup -1} to 35.4 dN cm{sup -1} and formed a stable and compact emulsion with an emulsifying activity of 74% with diesel oil, when grown on dextrose. These findings indicate that this isolate may be useful for bioremediation of sites contaminated with aromatic hydrocarbons. (orig.)

  18. MG53-IRS-1 (Mitsugumin 53-Insulin Receptor Substrate-1) Interaction Disruptor Sensitizes Insulin Signaling in Skeletal Muscle.

    Science.gov (United States)

    Lee, Hyun; Park, Jung-Jin; Nguyen, Nga; Park, Jun Sub; Hong, Jin; Kim, Seung-Hyeob; Song, Woon Young; Kim, Hak Joong; Choi, Kwangman; Cho, Sungchan; Lee, Jae-Seon; Kim, Bong-Woo; Ko, Young-Gyu

    2016-12-23

    Mitsugumin 53 (MG53) is an E3 ligase that interacts with and ubiquitinates insulin receptor substrate-1 (IRS-1) in skeletal muscle; thus, an MG53-IRS-1 interaction disruptor (MID), which potentially sensitizes insulin signaling with an elevated level of IRS-1 in skeletal muscle, is an excellent candidate for treating insulin resistance. To screen for an MID, we developed a bimolecular luminescence complementation system using an N-terminal luciferase fragment fused with IRS-1 and a C-terminal luciferase fragment fused with an MG53 C14A mutant that binds to IRS-1 but does not have E3 ligase activity. An MID, which was discovered using the bimolecular luminescence complementation system, disrupted the molecular association of MG53 with IRS-1, thus abolishing MG53-mediated IRS-1 ubiquitination and degradation. Thus, the MID sensitized insulin signaling and increased insulin-elicited glucose uptake with an elevated level of IRS-1 in C2C12 myotubes. These data indicate that this MID holds promise as a drug candidate for treating insulin resistance. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  19. No association of the IRS1 and PAX4 genes with type I diabetes

    DEFF Research Database (Denmark)

    Bergholdt, R.; Brorsson, C.; Boehm, B.

    2009-01-01

    To reassess earlier suggested type I diabetes (T1D) associations of the insulin receptor substrate 1 (IRS1) and the paired domain 4 gene (PAX4) genes, the Type I Diabetes Genetics Consortium (T1DGC) evaluated single-nucleotide polymorphisms (SNPs) covering the two genomic regions. Sixteen SNPs we...... of tagging SNPs, more than one genotyping platform in high throughput studies, and sufficient power to draw solid conclusions in genetic studies of human complex diseases. Genes and Immunity (2009) 10, S49-S53; doi:10.1038/gene.2009.91 Udgivelsesdato: 2009/12...

  20. Crossing scheme and orbit correction in IR1/5 for HL-LHC

    CERN Document Server

    Fitterer, M; Giovannozzi, M; De Maria, R

    2015-01-01

    In this paper we review the orbit correction strategy and crossing scheme adjustment for the HL-LHC orbit correctors in IR1/5 in view of the new optics and layout version HLLHCV1.1. To this aim the results of the studies conducted for the previous layout HLLHCV1.0 are also presented here. The main objectives are to optimize the crossing scheme, in particular to reduce the strength of the orbit correctors at D2, and to obtain an estimate for the required strength to compensate orbit distortions due to various sources.

  1. "Dust, Ice, and Gas In Time" (DIGIT) Herschel Observations of GSS30-IRS1 in Ophiuchus

    CERN Document Server

    Je, Hyerin; Lee, Seokho; Green, Joel D; Evans, Neal J

    2015-01-01

    As a part of the "Dust, Ice, and Gas In Time" (DIGIT) key program on Herschel, we observed GSS30-IRS1, a Class I protostar located in Ophiuchus (d = 120 pc), with Herschel/Photodetector Array Camera and Spectrometer (PACS). More than 70 lines were detected within a wavelength range from 50 micron to 200 micron, including CO, H2O, OH, and two atomic [O I] lines at 63 and 145 micron. The [C II] line, known as a tracer of externally heated gas by the interstellar radiation field, is also detected at 158 micron. All lines, except [O I] and [C II], are detected only at the central spaxel of 9.4" X 9.4". The [O I] emissions are extended along a NE-SW orientation, and the [C II] line is detected over all spaxels, indicative of external PDR. The total [C II] intensity around GSS30 reveals that the far-ultraviolet radiation field is in the range of 3 to 20 G0, where G0 is in units of the Habing Field, 1.6 X 10^{-3} erg cm^{-2} s^{-1}. This enhanced external radiation field heats the envelope of GSS30-IRS1, causing the...

  2. Up-regulated miR-145 expression inhibits porcine preadipocytes differentiation by targeting IRS1.

    Science.gov (United States)

    Guo, Yunxue; Chen, Yaosheng; Zhang, Yun; Zhang, Yue; Chen, Luxi; Mo, Delin

    2012-01-01

    Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.

  3. New infrared observations of IRS1, IRS3, and the adjacent nebula in the OMC-2 cluster

    Science.gov (United States)

    Pendleton, Y.; Werner, M.; Capps, R.; Dinerstein, H. L.

    1984-01-01

    Observations of the infrared cluster of low luminosity protostars in Orion Molecular Cloud 2 (OMC2) are reported. Results show that the asymmetric distribution of the extended emission seen about IRS1 is another infrared reflection nebula. Energy distributions show that the spectral shape is fairly constant throughout the nebula which indicates there is little internal extinction within this region. Integrated surface brightness values show that the nebula is 5 times brighter than IRS1 at K. Energy distributions show that IRS1 has a more pronounced ice band absorption feature at 3.1 micron; suggesting that there is more extinction along the direct line of sight to IRS1 than along a line from IRS1 to the scattering grains and then to the observer. The distribution of the extended emission around IRS1 is similar to the reflection nebula seen in NGC 7538 (Werner et al. 1979). The asymmetric shapes of the two nebulae are similar and in each case there is excess extinction along line of sight to the illuminating source.

  4. New infrared observations of IRS1, IRS3, and the adjacent nebula in the OMC-2 cluster

    Science.gov (United States)

    Pendleton, Y.; Werner, M.; Capps, R.; Dinerstein, H. L.

    1984-01-01

    Observations of the infrared cluster of low luminosity protostars in Orion Molecular Cloud 2 (OMC2) are reported. Results show that the asymmetric distribution of the extended emission seen about IRS1 is another infrared reflection nebula. Energy distributions show that the spectral shape is fairly constant throughout the nebula which indicates there is little internal extinction within this region. Integrated surface brightness values show that the nebula is 5 times brighter than IRS1 at K. Energy distributions show that IRS1 has a more pronounced ice band absorption feature at 3.1 micron; suggesting that there is more extinction along the direct line of sight to IRS1 than along a line from IRS1 to the scattering grains and then to the observer. The distribution of the extended emission around IRS1 is similar to the reflection nebula seen in NGC 7538 (Werner et al. 1979). The asymmetric shapes of the two nebulae are similar and in each case there is excess extinction along line of sight to the illuminating source.

  5. Tanshinone I alleviates insulin resistance in type 2 diabetes mellitus rats through IRS-1 pathway.

    Science.gov (United States)

    Wei, Ying; Gao, Jiaqi; Qin, Lingling; Xu, Yunling; Wang, Dongchao; Shi, Haoxia; Xu, Tunhai; Liu, Tonghua

    2017-09-01

    Tanshinone I from tanshen has been used in traditional Chinese medicine for treating cardiovascular diseases and inflammatory diseases. Given the link between inflammation and Type 2 diabetes mellitus (T2DM), we suspect that tanshinone I may have a beneficial effect on T2DM. This study was to investigate the potential effects of tanshinone I on T2DM and its underlying mechanism. T2DM was thus induced in Sprague-Dawley (SD) rats using streptozotocin (STZ) and high-fat diet. It was observed that T2DM rats had higher levels of total cholesterol (TC), nonesterified fatty acids (NEFAs), total triglyceride (TG) and total low density lipoprotein cholesterol (LDL-C) compared with normal, healthy SD rats. Treatment with tanshinone I decreased these levels and lowered blood glucose level in T2DM rats. In addition, enzyme-linked immunosorbent assay (ELISA) analysis showed that T2DM rats had elevated levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Furthermore, Western blot analysis revealed that T2DM rats had enhanced nuclear translocation of NF-κB as well as elevated phosphorylation of Ser307 in IRS-1(insulin receptor substrate 1). Treatment by tanshinone I lowered the levels of IL-6 and TNF-α, decreased nuclear translocation of NF-κB as well as phosphorylation of Ser307 in IRS-1. These results demonstrated that tanshinone I could alleviate T2DM syndrome in rats. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  6. GenBank

    Science.gov (United States)

    Benson, Dennis A.; Cavanaugh, Mark; Clark, Karen; Karsch-Mizrachi, Ilene; Lipman, David J.; Ostell, James; Sayers, Eric W.

    2017-01-01

    GenBank® (www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for 370 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole genome shotgun (WGS) and environmental sampling projects. Most submissions are made using the web-based BankIt or the NCBI Submission Portal. GenBank staff assign accession numbers upon data receipt. Daily data exchange with the European Nucleotide Archive (ENA) and the DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through the NCBI Nucleotide database, which links to related information such as taxonomy, genomes, protein sequences and structures, and biomedical journal literature in PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. Recent updates include changes to policies regarding sequence identifiers, an improved 16S submission wizard, targeted loci studies, the ability to submit methylation and BioNano mapping files, and a database of anti-microbial resistance genes. PMID:27899564

  7. Dilinoleoyl-phosphatidic acid mediates reduced IRS-1 tyrosine phosphorylation in rat skeletal muscle cells and mouse muscle.

    Science.gov (United States)

    Cazzolli, R; Mitchell, T W; Burchfield, J G; Pedersen, D J; Turner, N; Biden, T J; Schmitz-Peiffer, C

    2007-08-01

    Insulin resistance in skeletal muscle is strongly associated with lipid oversupply, but the intracellular metabolites and underlying mechanisms are unclear. We therefore sought to identify the lipid intermediates through which the common unsaturated fatty acid linoleate causes defects in IRS-1 signalling in L6 myotubes and mouse skeletal muscle. Cells were pre-treated with 1 mmol/l linoleate for 24 h. Subsequent insulin-stimulated IRS-1 tyrosine phosphorylation and its association with the p85 subunit of phosphatidylinositol 3-kinase were determined by immunoblotting. Intracellular lipid species and protein kinase C activation were modulated by overexpression of diacylglycerol kinase epsilon, which preferentially converts unsaturated diacylglycerol into phosphatidic acid, or by inhibition of lysophosphatidic acid acyl transferase with lisofylline, which reduces phosphatidic acid synthesis. Phosphatidic acid species in linoleate-treated cells or muscle from insulin-resistant mice fed a safflower oil-based high-fat diet that was rich in linoleate were analysed by mass spectrometry. Linoleate pretreatment reduced IRS-1 tyrosine phosphorylation and p85 association. Overexpression of diacylglycerol kinase epsilon reversed the activation of protein kinase C isoforms by linoleate, but paradoxically further diminished IRS-1 tyrosine phosphorylation. Conversely, lisofylline treatment restored IRS-1 phosphorylation. Mass spectrometry indicated that the dilinoleoyl-phosphatidic acid content increased from undetectable levels to almost 20% of total phosphatidic acid in L6 cells and to 8% of total in the muscle of mice fed a high-fat diet. Micelles containing dilinoleoyl-phosphatidic acid specifically inhibited IRS-1 tyrosine phosphorylation and glycogen synthesis in L6 cells. These data indicate that linoleate-derived phosphatidic acid is a novel lipid species that contributes independently of protein kinase C to IRS-1 signalling defects in muscle cells in response to lipid

  8. A novel IRS-1-associated protein, DGKζ regulates GLUT4 translocation in 3T3-L1 adipocytes.

    Science.gov (United States)

    Liu, TingYu; Yu, BuChin; Kakino, Mamoru; Fujimoto, Hitoshi; Ando, Yasutoshi; Hakuno, Fumihiko; Takahashi, Shin-Ichiro

    2016-10-14

    Insulin receptor substrates (IRSs) are major targets of insulin receptor tyrosine kinases. Here we identified diacylglycerol kinase zeta (DGKζ) as an IRS-1-associated protein, and examined roles of DGKζ in glucose transporter 4 (GLUT4) translocation to the plasma membrane. When DGKζ was knocked-down in 3T3-L1 adipocytes, insulin-induced GLUT4 translocation was inhibited without affecting other mediators of insulin-dependent signaling. Similarly, knockdown of phosphatidylinositol 4-phosphate 5-kinase 1α (PIP5K1α), which had been reported to interact with DGKζ, also inhibited insulin-induced GLUT4 translocation. Moreover, DGKζ interacted with IRS-1 without insulin stimulation, but insulin stimulation decreased this interaction. Over-expression of sDGKζ (short-form DGKζ), which competed out DGKζ from IRS-1, enhanced GLUT4 translocation without insulin stimulation. Taking these results together with the data showing that cellular PIP5K activity was correlated with GLUT4 translocation ability, we concluded that IRS-1-associated DGKζ prevents GLUT4 translocation in the absence of insulin and that the DGKζ dissociated from IRS-1 by insulin stimulation enhances GLUT4 translocation through PIP5K1α activity.

  9. APPL1 potentiates insulin sensitivity by facilitating the binding of IRS1/2 to the insulin receptor.

    Science.gov (United States)

    Ryu, Jiyoon; Galan, Amanda K; Xin, Xiaoban; Dong, Feng; Abdul-Ghani, Muhammad A; Zhou, Lijun; Wang, Changhua; Li, Cuiling; Holmes, Bekke M; Sloane, Lauren B; Austad, Steven N; Guo, Shaodong; Musi, Nicolas; DeFronzo, Ralph A; Deng, Chuxia; White, Morris F; Liu, Feng; Dong, Lily Q

    2014-05-22

    Binding of insulin receptor substrate proteins 1 and 2 (IRS1/2) to the insulin receptor (IR) is essential for the regulation of insulin sensitivity and energy homeostasis. However, the mechanism of IRS1/2 recruitment to the IR remains elusive. Here, we identify adaptor protein APPL1 as a critical molecule that promotes IRS1/2-IR interaction. APPL1 forms a complex with IRS1/2 under basal conditions, and this complex is then recruited to the IR in response to insulin or adiponectin stimulation. The interaction between APPL1 and IR depends on insulin- or adiponectin-stimulated APPL1 phosphorylation, which is greatly reduced in insulin target tissues in obese mice. appl1 deletion in mice consistently leads to systemic insulin resistance and a significant reduction in insulin-stimulated IRS1/2, but not IR, tyrosine phosphorylation, indicating that APPL1 sensitizes insulin signaling by acting at a site downstream of the IR. Our study uncovers a mechanism regulating insulin signaling and crosstalk between the insulin and adiponectin pathways.

  10. APPL1 Potentiates Insulin Sensitivity by Facilitating the Binding of IRS1/2 to the Insulin Receptor

    Directory of Open Access Journals (Sweden)

    Jiyoon Ryu

    2014-05-01

    Full Text Available Binding of insulin receptor substrate proteins 1 and 2 (IRS1/2 to the insulin receptor (IR is essential for the regulation of insulin sensitivity and energy homeostasis. However, the mechanism of IRS1/2 recruitment to the IR remains elusive. Here, we identify adaptor protein APPL1 as a critical molecule that promotes IRS1/2-IR interaction. APPL1 forms a complex with IRS1/2 under basal conditions, and this complex is then recruited to the IR in response to insulin or adiponectin stimulation. The interaction between APPL1 and IR depends on insulin- or adiponectin-stimulated APPL1 phosphorylation, which is greatly reduced in insulin target tissues in obese mice. appl1 deletion in mice consistently leads to systemic insulin resistance and a significant reduction in insulin-stimulated IRS1/2, but not IR, tyrosine phosphorylation, indicating that APPL1 sensitizes insulin signaling by acting at a site downstream of the IR. Our study uncovers a mechanism regulating insulin signaling and crosstalk between the insulin and adiponectin pathways.

  11. Regular exercise enhances insulin activation of IRS-1-associated PI3-kinase in human skeletal muscle.

    Science.gov (United States)

    Kirwan, J P; del Aguila, L F; Hernandez, J M; Williamson, D L; O'Gorman, D J; Lewis, R; Krishnan, R K

    2000-02-01

    Insulin action in skeletal muscle is enhanced by regular exercise. Whether insulin signaling in human skeletal muscle is affected by habitual exercise is not well understood. Phosphatidylinositol 3-kinase (PI3-kinase) activation is an important step in the insulin-signaling pathway and appears to regulate glucose metabolism via GLUT-4 translocation in skeletal muscle. To examine the effects of regular exercise on PI3-kinase activation, 2-h hyperinsulinemic (40 mU. m(-2). min(-1))-euglycemic (5.0 mM) clamps were performed on eight healthy exercise-trained [24 +/- 1 yr, 71.8 +/- 2.0 kg, maximal O(2) uptake (VO(2 max)) of 56.1 +/- 2.5 ml. kg(-1). min(-1)] and eight healthy sedentary men and women (24 +/- 1 yr, 64.7 +/- 4.4 kg, VO(2 max) of 44.4 +/- 2.7 ml. kg(-1). min(-1)). A [6, 6-(2)H]glucose tracer was used to measure hepatic glucose output. A muscle biopsy was obtained from the vastus lateralis muscle at basal and at 2 h of hyperinsulinemia to measure insulin receptor substrate-1(IRS-1)-associated PI3-kinase activation. Insulin concentrations during hyperinsulinemia were similar for both groups (293 +/- 22 and 311 +/- 22 pM for trained and sedentary, respectively). Insulin-mediated glucose disposal rates (GDR) were greater (P exercise-trained compared with the sedentary control group (9.22 +/- 0.95 vs. 6.36 +/- 0.57 mg. kg fat-free mass(-1). min(-1)). Insulin-stimulated PI3-kinase activation was also greater (P < 0.004) in the trained compared with the sedentary group (3.8 +/- 0.5- vs. 1.8 +/- 0.2-fold increase from basal). Endurance capacity (VO(2 max)) was positively correlated with PI3-kinase activation (r = 0.53, P < 0.04). There was no correlation between PI3-kinase and muscle morphology. However, increases in GDR were positively related to PI3-kinase activation (r = 0.60, P < 0.02). We conclude that regular exercise leads to greater insulin-stimulated IRS-1-associated PI3-kinase activation in human skeletal muscle, thus facilitating enhanced insulin

  12. DETEKSI GEN-GEN PENYANDI FAKTOR VIRULENSI PADA BAKTERI VIBRIO

    Directory of Open Access Journals (Sweden)

    Ince Ayu Khairani Kadriah

    2011-04-01

    menggunakan isolat bakteri yang diisolasi dari budidaya udang windu di berbagai daerah di Sulawesi Selatan dan Jawa. Pada penelitian ini digunakan primer spesifik untuk mendeteksi gen-gen virulen toxR gene, hemolysin (vvh gene, dan GyrB gene dengan metode PCR. Dari 35 isolat yang diisolasi, 20 isolat terdeteksi memiliki gen virulensi dan 8 di antaranya memiliki dua gen virulen. Spesies bakteri yang memiliki gen virulen adalah: V.harveyi, V. parahaemolyticus, V. mimicus, dan V. campbelli

  13. Ab initio study of structural, electronic, and thermal properties of Ir1-xRhx alloys

    Directory of Open Access Journals (Sweden)

    Sh. Ahmed

    2015-06-01

    Full Text Available The structural, electronic, mechanical and thermal properties of Ir1-xRhx alloys was studied systematically using ab initio density functional theory at different concentration (x = 0.00, 0.25, 0.50, 0.75, 1.00. The Special Quasirandom Structure method was used to make the alloys with FCC structure with four atoms per unit cell. The ground state properties such as lattice constant and bulk modulus were calculated to find the equilibrium atomic position for stable alloys. The calculated ground state properties are in good agreement with the experimental and previously presented other theoretical data. The electronic band structure and density of states were calculated to study the electronic properties for these alloys at different concentration. The electronic properties substantiate metallic behavior of alloys. The first principle density functional perturbation theory as implemented in quasiharmonic approximation was used for the calculation of thermal properties. We have calculated the thermal properties such the Debye temperatures, vibration energy, entropy, constant-volume specific heat and internal energy. The ab initio linear-response method was used for phonon densities of states calculations.

  14. Energy Deposition in the LHC Insertion Regions IR1 and IR5

    CERN Document Server

    Hoa, C; Wildner, E

    2008-01-01

    Proton-proton collision debris coming out from the Interaction Point (IP) impacts the superconducting magnets of the insertion region and induces energy deposition in the coils. This is a critical aspect to evaluate regarding quench limit in the superconducting magnets. The study presents an estimation of the energy deposition in the insertion regions IR1 (ATLAS) and IR5 (CMS) for version 6.5 of the LHC layout, with a baseline nominal luminosity of L=1034 s-1 cm-2 for proton-proton collisions at 14 TeV center of mass energy. All essential components in the insertion regions up to 60 m from the interaction point have been implemented with a detailed description of their geometry, material and magnetic field. Total heat loads and power density distributions are evaluated in the components of the inner triplet, including also the TAS absorbers and the corrector magnets. The results are obtained using FLUKA, a Monte Carlo code modelling particle interaction and transport [1-2].

  15. New infrared observations of IRS1, IRS3 and the adjacent nebula in the OMC-2 cluster

    Science.gov (United States)

    Pendleton, Y.; Werner, M.; Capps, R.; Dinerstein, H. L.

    1984-01-01

    Near infrared reflection nebulae are often observed around embedded protostellar objects. New observations of the infrared cluster of low luminosity protostars in Orion Molecular Cloud 2 (OMC2) are reported. The asymmetric distribution of the extended emission seen about IRS1 is in fact another infrared reflection nebulae. Observations of near infrared polarimetry, photometry, and spectrophotometry were carried out.

  16. Effects of chromium supplementation on IRS-1 expression of skeletal muscle in diabetic rats%补铬对糖尿病大鼠骨骼肌IRS-1基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    孙忠; 吴蕴棠; 车素萍; 王夏; 郭刚

    2005-01-01

    目的研究补铬对糖尿病大鼠骨骼肌代谢相关基因表达的影响.方法对前期研究分离到的与补铬有关的糖尿病大鼠差异显示的基因片段进行克隆、测序,并进行同源性分析.根据待检测的基因序列进行引物设计,进行RT-PCR检测.用激光密度扫描仪进行光密度扫描分析,以目的基因与参考基因的灰度比值反映其表达水平.结果经过克隆、测序、同源性比较,差显片段Cr-5的碱基序列与IRS-1同源性为100%.糖尿病对照组IRS-1 mRNA表达量(0.791±0.038)低于正常对照组(0.892±0.053,P<0.05),糖尿病补铬组IRS-1 mRNA的表达量(0.822±0.066)与糖尿病对照组相比有所恢复,但尚未恢复到正常水平(P>0.05).结论补铬对糖尿病大鼠骨骼肌IRS-1mRNA表达有上调趋势,Cr-5将作为候选基因有待于进一步研究.

  17. GenBank

    OpenAIRE

    Burks, Christian; Cassidy, Maxxwell; Cinkosky, Michael J.; Cumella, Karen E.; Gilna, Paul; Hayden, Jamie E.-D.; Keen, Gifford M.; Kelley, Tom A.; Kelly, Michael; Kristofferson, David; Ryals, Julie

    1991-01-01

    The GenBank nucleotide sequence database now contains sequence data and associated annotation corresponding to 56,000,000 nucleotides in 45,000 entries. The input stream of data coming into the database has largely been shifted to direct submissions from the scientific community on electronic media. The data have been installed in a relational database management system and are made available in this form through on-line access, and through various network and off-line computer-readable media...

  18. XIAO Pei-gen

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Academician of Chinese Academy of Engineering Editor-in-chief of Chinese Herbal Medicines(CHM)Honorary director of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing100193,China Tel/Fax:+86-10-62894462 E-mail:xiaopg@public.bta.net.cn Professor XIAO Pei-gen is the founder of the Institute of Medicinal Plant Development(IMPLAD),Chinese Academy of Medical Sciences(CAMS).He is also one of the founders and leading

  19. Insulin Receptor Substrate-1 (IRS-1 Gly927Arg: Correlation with Gestational Diabetes Mellitus in Saudi Women

    Directory of Open Access Journals (Sweden)

    Khalid Khalaf Alharbi

    2014-01-01

    Full Text Available Pregnant women with gestational diabetes mellitus (GDM and type 2 diabetes mellitus (T2DM share a common pathophysiology associated with similar risk factors. Genetic variants used to determine the risk of developing T2DM might also be associated with the prevalence of GDM. The aim of the present study was to scrutinize the relationship between the G972R polymorphism of the insulin receptor substrate-1 (IRS-1 gene with GDM in the Saudi female population. This is a case-control study that monitored 500 Saudi women. Subjects with GDM (n=200 were compared with non-GDM (n=300 controls. We opted to evaluate rs1801278 polymorphism in the IRS1 gene, which plays a critical role in the insulin-signaling pathway. Genotyping was performed with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP method. The frequency of the rs1801278 polymorphism was significantly higher in women with GDM than in women with non-GDM (for TT + CT versus CC: P=0.02. Additionally, there was a significant increase in the frequency of the Arg-encoding mutant allele from GDM to non-GDM (for T versus C: P=0.01. Our results suggest that the rs1801278 polymorphism in the IRS-1 gene is involved in the occurrence of GDM in the Saudi population.

  20. Insulin receptor substrate-1 (IRS-1) Gly927Arg: correlation with gestational diabetes mellitus in Saudi women.

    Science.gov (United States)

    Alharbi, Khalid Khalaf; Khan, Imran Ali; Abotalib, Zeinab; Al-Hakeem, Malak Mohammed

    2014-01-01

    Pregnant women with gestational diabetes mellitus (GDM) and type 2 diabetes mellitus (T2DM) share a common pathophysiology associated with similar risk factors. Genetic variants used to determine the risk of developing T2DM might also be associated with the prevalence of GDM. The aim of the present study was to scrutinize the relationship between the G972R polymorphism of the insulin receptor substrate-1 (IRS-1) gene with GDM in the Saudi female population. This is a case-control study that monitored 500 Saudi women. Subjects with GDM (n = 200) were compared with non-GDM (n = 300) controls. We opted to evaluate rs1801278 polymorphism in the IRS1 gene, which plays a critical role in the insulin-signaling pathway. Genotyping was performed with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The frequency of the rs1801278 polymorphism was significantly higher in women with GDM than in women with non-GDM (for TT + CT versus CC: P = 0.02). Additionally, there was a significant increase in the frequency of the Arg-encoding mutant allele from GDM to non-GDM (for T versus C: P = 0.01). Our results suggest that the rs1801278 polymorphism in the IRS-1 gene is involved in the occurrence of GDM in the Saudi population.

  1. Hepatitis B virus X protein impairs hepatic insulin signaling through degradation of IRS1 and induction of SOCS3.

    Directory of Open Access Journals (Sweden)

    KyeongJin Kim

    Full Text Available BACKGROUND: Hepatitis B virus (HBV is a major cause of chronic liver diseases, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. The role of HCV in associations with insulin signaling has been elucidated. However, the pathogenesis of HBV-associated insulin signaling remains to be clearly characterized. Therefore, we have attempted to determine the mechanisms underlying the HBV-associated impairment of insulin signaling. METHODOLOGY: The expressions of insulin signaling components were investigated in HBx-transgenic mice, HBx-constitutive expressing cells, and transiently HBx-transfected cells. Protein and gene expression was examined by Western blot, immunohistochemistry, RT-PCR, and promoter assay. Protein-protein interaction was detected by coimmunoprecipitation. PRINCIPAL FINDINGS: HBx induced a reduction in the expression of IRS1, and a potent proteasomal inhibitor blocked the downregulation of IRS1. Additionally, HBx enhanced the expression of SOCS3 and induced IRS1 ubiquitination. Also, C/EBPalpha and STAT3 were involved in the HBx-induced expression of SOCS3. HBx interfered with insulin signaling activation and recovered the insulin-mediated downregulation of gluconeogenic genes. CONCLUSIONS/SIGNIFICANCE: These results provide direct experimental evidences for the contribution of HBx in the impairment of insulin signaling.

  2. Identification of IRS-1 Ser-1101 as a target of S6K1 in nutrient- and obesity-induced insulin resistance.

    Science.gov (United States)

    Tremblay, Frédéric; Brûlé, Sophie; Hee Um, Sung; Li, Yu; Masuda, Kohei; Roden, Michael; Sun, Xiao Jian; Krebs, Michael; Polakiewicz, Roberto D; Thomas, George; Marette, André

    2007-08-28

    S6K1 has emerged as a critical signaling component in the development of insulin resistance through phosphorylation and inhibition of IRS-1 function. This effect can be triggered directly by nutrients such as amino acids or by insulin through a homeostatic negative-feedback loop. However, the role of S6K1 in mediating IRS-1 phosphorylation in a physiological setting of nutrient overload is unresolved. Here we show that S6K1 directly phosphorylates IRS-1 Ser-1101 in vitro in the C-terminal domain of the protein and that mutation of this site largely blocks the ability of amino acids to suppress IRS-1 tyrosine and Akt phosphorylation. Consistent with this finding, phosphorylation of IRS-1 Ser-1101 is increased in the liver of obese db/db and wild-type, but not S6K1(-/-), mice maintained on a high-fat diet and is blocked by siRNA knockdown of S6K1 protein. Finally, infusion of amino acids in humans leads to the concomitant activation of S6K1, phosphorylation of IRS-1 Ser-1101, a reduction in IRS-1 function, and insulin resistance in skeletal muscle. These findings indicate that nutrient- and hormonal-dependent activation of S6K1 causes insulin resistance in mice and humans, in part, by mediating IRS-1 Ser-1101 phosphorylation.

  3. A nonsynonymous polymorphism in IRS1 modifies risk of developing breast and ovarian cancers in BRCA1 and ovarian cancer in BRCA2 mutation carriers

    DEFF Research Database (Denmark)

    Ding, Yuan C; McGuffog, Lesley; Healey, Sue

    2012-01-01

    We previously reported significant associations between genetic variants in insulin receptor substrate 1 (IRS1) and breast cancer risk in women carrying BRCA1 mutations. The objectives of this study were to investigate whether the IRS1 variants modified ovarian cancer risk and were associated wit...

  4. 秦川牛IRS-1基因多态性与体尺和肉质性状的相关性%Association of IRS-1 Gene Polymorphism with Body Measurement and Meat Quality Traits in Qinchuan Cattle

    Institute of Scientific and Technical Information of China (English)

    马向辉; 姜碧杰; 詹小立; 白银萍; 昝林森; 高建斌; 杨宁; 朱光星; 成功; 王洪宝; 郝瑞杰; 付常振

    2012-01-01

    旨在研究秦川牛IRS-1基因外显子的多态性,及其与秦川牛体尺和肉质性状的相关性.选取384头同等饲养条件下的18~24月龄健康秦川牛母牛为研究对象,采用PCR-SSCP技术结合DNA测序技术检测IRS-1基因外显子上存在的SNPs位点,并将其与秦川牛体尺和肉质性状进行关联分析.经DNA测序发现,在IRS-1基因外显子的2 346(B1位点)和2 394 bp处(B2位点)分别发生了G→A和C→G突变,经分析发现,分别为氨基酸序列第782处Glu和798处Leu的同义突变.通过SSCP带型分析分别出现CC、CD和AA、AB 2种基因型,均未检测出第3种纯合基因型;卡方检验显示,B1位点在所检测牛群中处于Hardy-Weinberg平衡状态(P>0.05),而B2位点则处于Hardy-Weinberg极度不平衡状态(P<0.01);且B1处CC基因型为优势基因型,C为优势等位基因,处于低度多态状态;B2处基因型AA为优势基因型,A为优势等位基因,处于低度多态状态;关联分析表明,B1位点不同基因型个体间在体斜长、腰角宽、胸围和眼肌面积方面差异显著(P<0.05),B2位点在腰角宽、胸深和背膘厚方面差异显著(P<0.05).将2个突变位点合并基因型进行单倍型分析发现,双杂合基因型个体(ABCD)除背膘厚和肌间脂肪含量外其他所分析的各性状均显著高于其他基因型组合,且多标记位点的组合效应值高于单标记位点.结果提示,IRS-1基因对秦川牛体尺及部分肉质性状有显著的影响,可以作为秦川肉牛新品系培育早期选择的候选基因,其中组合ABCD可能是影响秦川牛体尺和胴体性状的最佳基因型组合.%The aim of this study was to study the polymorphisms on exons of IRS-1 gene and their association with several body measurements and meat quality traits in Qinchuan cattle. The polymorphisms on exons of bovine IRS-1 gene was detected by using PCR-SSCP and DNA sequencing technology. 384 Qinchuan cattle with 18 to 24 months old were

  5. Properties of Sin, Gen, and SinGen clusters

    Science.gov (United States)

    Dong, Yi; ur Rehman, Habib; Springborg, Michael

    2015-01-01

    The structures of Sin, Gen, and SinGen clusters with up to 44 atoms have been determined theoretically using an unbiased structure-optimization method in combination with a parametrized, density-functional description of the total energy for a given structure. By analyzing the total energy in detail, particularly stable clusters are identified. Moreover, general trends in the structures are identified with the help of specifically constructed descriptors.

  6. Inferring the evolutionary stages of the internal structures of NGC 7538 S and IRS1 from chemistry

    Science.gov (United States)

    Feng, S.; Beuther, H.; Semenov, D.; Henning, Th.; Linz, H.; Mills, E. A. C.; Teague, R.

    2016-09-01

    Context. Radiative feedback of young (proto)stars and gas dynamics including gravitational collapse and outflows are important in high-mass star-forming regions (HMSFRs), for the reason that they may leave footprints on the gas density and temperature distributions, the velocity profile, and the chemical abundances. Aims: We unambiguously diagnose the detailed physical mechanisms and the evolutionary status of HMSFRs. Methods: We performed 0.4'' (~1000 AU) resolution observations at 1.37 mm towards two HMSFRs, NGC 7538 S and IRS1, using the Plateau de Bure Interferometre (PdBI). The observations covered abundant molecular lines, including tracers of gas column density, hot molecular cores, shocks, and complex organic molecules. We present a joint analysis of the 1.37 mm continuum emission and the line intensity of 15 molecular species (including 22 isotopologues). Assuming local thermal equilibrium (LTE), we derived molecular column densities and molecular abundances for each internal gas substructure that is spatially resolved. These derived quantities are compared with a suite of 1D gas-grain models. Results: NGC 7538 S is resolved into at least three dense gas condensations. Despite the comparable continuum intensity of these condensations, their differing molecular line emission is suggestive of an overall chemical evolutionary trend from the northeast to the southwest. Line emission from MM1 is consistent with a chemically evolved hot molecular core (HMC), whereas MM3 remains a prestellar candidate that only exhibits emission of lower-excitation lines. The condensation MM2, located between MM1 and MM3, shows an intermediate chemical evolutionary status. Since these three condensations are embedded within the same parent gas core, their differing chemical properties are most likely due to the different warm-up histories, rather than the different dynamic timescales. Despite remaining spatially unresolved, in IRS1 we detect abundant complex organic molecules (e

  7. NGC 7538 : Multiwavelength Study of Stellar Cluster Regions associated with IRS 1-3 and IRS 9 sources

    OpenAIRE

    Mallick, K. K.; Ojha, D. K.; Tamura, M; Pandey, A.K.(Indian Institute of Technology Bombay (IIT), Mumbai, India); Dib, S; S.K Ghosh; Sunada, K.; Zinchenko, I.; Pirogov, L.; Tsujimoto, M

    2014-01-01

    We present deep and high-resolution (FWHM ~ 0.4 arcsec) near-infrared (NIR) imaging observations of the NGC 7538 IRS 1-3 region (in JHK bands), and IRS 9 region (in HK bands) using the 8.2m Subaru telescope. The NIR analysis is complemented with GMRT low-frequency observations at 325, 610, and 1280 MHz, molecular line observations of H13CO+ (J=1-0), and archival Chandra X-ray observations. Using the 'J-H/H-K' diagram, 144 Class II and 24 Class I young stellar object (YSO) candidates are ident...

  8. Demonstrated brain insulin resistance in Alzheimer’s disease patients is associated with IGF-1 resistance, IRS-1 dysregulation, and cognitive decline

    Science.gov (United States)

    Talbot, Konrad; Wang, Hoau-Yan; Kazi, Hala; Han, Li-Ying; Bakshi, Kalindi P.; Stucky, Andres; Fuino, Robert L.; Kawaguchi, Krista R.; Samoyedny, Andrew J.; Wilson, Robert S.; Arvanitakis, Zoe; Schneider, Julie A.; Wolf, Bryan A.; Bennett, David A.; Trojanowski, John Q.; Arnold, Steven E.

    2012-01-01

    While a potential causal factor in Alzheimer’s disease (AD), brain insulin resistance has not been demonstrated directly in that disorder. We provide such a demonstration here by showing that the hippocampal formation (HF) and, to a lesser degree, the cerebellar cortex in AD cases without diabetes exhibit markedly reduced responses to insulin signaling in the IR→IRS-1→PI3K signaling pathway with greatly reduced responses to IGF-1 in the IGF-1R→IRS-2→PI3K signaling pathway. Reduced insulin responses were maximal at the level of IRS-1 and were consistently associated with basal elevations in IRS-1 phosphorylated at serine 616 (IRS-1 pS616) and IRS-1 pS636/639. In the HF, these candidate biomarkers of brain insulin resistance increased commonly and progressively from normal cases to mild cognitively impaired cases to AD cases regardless of diabetes or APOE ε4 status. Levels of IRS-1 pS616 and IRS-1 pS636/639 and their activated kinases correlated positively with those of oligomeric Aβ plaques and were negatively associated with episodic and working memory, even after adjusting for Aβ plaques, neurofibrillary tangles, and APOE ε4. Brain insulin resistance thus appears to be an early and common feature of AD, a phenomenon accompanied by IGF-1 resistance and closely associated with IRS-1 dysfunction potentially triggered by Aβ oligomers and yet promoting cognitive decline independent of classic AD pathology. PMID:22476197

  9. Effect of Berberine,Catalpol and Their Combination on the Expression of Glut-4,IRS-1,IRS-1 Ser307 Phosphorylation in Insulin Resistant 3T3-L1 Adipocytes%小檗碱与梓醇及其配伍对胰岛素抵抗3T3-L1脂肪细胞Glut-4、IRS-1IRS-1 Ser307磷酸化蛋白表达的影响

    Institute of Scientific and Technical Information of China (English)

    汪忠煜; 杨明炜; 陈立; 刘艳娟; 陆付耳; 黄光英

    2008-01-01

    目的: 观察梓醇与小檗碱及其配伍对胰岛素抵抗3T3-L1脂肪细胞葡萄糖消耗及这一过程中葡萄糖转运子-4(Glut-4)、胰岛素受体底物-1(IRS-1)和胰岛素受体底物-1丝氨酸307(IRS-1 Ser307)磷酸化蛋白表达的影响.方法: 采用高糖联合高胰岛素诱导3T3-L1脂肪细胞产生胰岛素抵抗,分别给予小檗碱、梓醇、小檗碱+梓醇、盐酸罗格列酮进行干预,以葡萄糖氧化酶法检测培养液中葡萄糖消耗量,以Western Blot法检测蛋白的表达.结果: 与模型组相比,小檗碱能增加培养液中葡萄糖的消耗(PIRS-1 Ser307磷酸化蛋白表达.结论: 小檗碱、梓醇及其配伍能改善胰岛素抵抗3T3-LI脂肪细胞的胰岛素敏感性,其作用机制与罗格列酮不同.

  10. Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: elimination of cross reactivity with adjacent Ser(358).

    Science.gov (United States)

    Waraich, Rizwana Sanaullah; Zaidi, Nousheen; Moeschel, Klaus; Beck, Alexander; Weigert, Cora; Voelter, Wolfgang; Kalbacher, Hubert; Lehmann, Rainer

    2008-11-07

    Antibodies that recognize specifically phosphorylated sites on proteins are widely utilized for studying the regulation and biological function of phosphoproteins. The proposed strategy is a powerful, analytical tool allowing the generation of phospho-site specific antibodies albeit adjacent phosphorylation sites are present. Here, we demonstrate the assessment and elimination of cross reactivity of phospho-site-specific-Ser(357) IRS-1 antibody. While determining the specificity of p-Ser(357) antiserum we came across the cross reactivity of the antiserum with adjacent Ser(358) which was successfully abolished by an improved immuno-purification method. The specificity of the purified antiserum was then verified by indirect ELISA, results of ELISA were also mirrored in the experiments carried out in BHK-IR cells using different mutants of IRS-1 carrying mutations at either Ser(357)/Ser(358)/Ser(357/358). Immuno-purified-p-Ser(357) did not react with IRS-1 Ala(357) and IRS-1 Ala(357/358). In conclusion, the present study describes generation and characterization of p-Ser(357) IRS-1 antibody, which reacts with IRS-1 in site specific and phosphorylation state-dependent manner without showing cross reactivity to adjacent Ser(358). This antibody can be effectively used to further clarify the inhibitory role of Ser(357) in insulin signal transduction.

  11. Melatonin rescues 3T3-L1 adipocytes from FFA-induced insulin resistance by inhibiting phosphorylation of IRS-1 on Ser307.

    Science.gov (United States)

    She, Meihua; Hou, Hongjie; Wang, Zongbao; Zhang, Chi; Laudon, Moshe; Yin, Weidong

    2014-08-01

    Melatonin is biosynthesized in the pineal gland and secreted into the bloodstream. Evidences indicate a role of melatonin in the regulation of glucose metabolism. The objective of this study was to investigate the effect of melatonin on insulin sensitivity in insulin resistant adipocytes. Following a preincubation with melatonin or vehicle for 30 min, insulin resistant cells of 3T3-L1 adipocytes were induced by palmitic acids (300 μM, 6 h). Our results showed that palmitic acids inhibited both the basal and insulin-stimulated uptake of [(3)H]-2-Deoxyglucose, down-regulated the levels of IRS-1 and GLUT-4. However, compared to the vehicle group, melatonin pre-treatment increased significantly the uptake of [(3)H]-2-Deoxyglucose as well as the level of GLUT-4, and decreased phosphorylated IRS-1 (Ser307) although total IRS-1 did not change significantly. These data suggest that palmitic acids impair insulin signal via down-regulating the expressions of IRS-1 and GLUT-4; whereas melatonin can ameliorate insulin sensitivity by inhibiting Ser307 phosphorylation in IRS-1 and increasing GLUT-4 expressions in insulin resistant 3T3-L1 adipocytes. We conclude that melatonin regulates the insulin sensitivity and glucose homeostasis via inhibiting Ser-phosphorylation and improving function of IRS-1.

  12. Oleanolic Acid Attenuates Insulin Resistance via NF-κB to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Ming Li

    2015-01-01

    Full Text Available The aim of our study is to elucidate the mechanisms of oleanolic acid (OA on insulin resistance (IR in HepG2 cells. HepG2 cells were induced with FFA as the insulin resistance model and were treated with OA. Then the glucose content and the levels of tumor necrosis factor-α (TNF-α and interleukin-6 (IL-6 were analyzed. Moreover, protein expression of nuclear factor kappa B (NF-κB, insulin receptor substrate 1(IRS1, and glucose transporter 4 (GLUT4 in cells treated with OA were measured by Western blot analysis. Additionally, IRS1 protein expression exposed to OA was detected after using pyrrolidine dithiocarbamate (PDTC.Our results revealed that OA decreased the glucose content in HepG2 cells in vitro. Moreover, OA reduced the levels of TNF-α and IL-6 and upregulated IRS1 and GLUT4 protein expression. Furthermore, OA also reduced NF-κB protein expression in insulin-resistant HepG2 cells. After blocking NF-κB, the expression of IRS1 protein had no obvious changes when treated with OA. OA attenuated insulin resistance and decreased the levels of TNF-α and IL-6. Meanwhile, OA decreased NF-κB protein expression and upregulated IRS1 and GLUT4 protein expression. Therefore, regulating the IRS1-GLUT4 pathway via NF-κB was the underlying mechanism of OA on insulin resistance.

  13. Structural and magnetic anomalies among the spin-chain compounds, Ca3Co1+Ir1-O6

    Indian Academy of Sciences (India)

    S Rayaprol; Kausik Sengupta; E V Sampathkumaran

    2003-10-01

    The results of X-ray diffraction, and ac and dc magnetisation as a function of temperature are reported for a new class of spin-chain oxides, Ca3Co1+Ir1-O6. While the = 0.0, 0.3, 0.5 and 1.0 are found to form in the K4CdCl6-derived rhombhohedral (space group $\\bar{3}$) structure, the = 0.7 composition is found to undergo a monoclinic distortion in contrast to a literature report. Apparently, the change in the crystal symmetry with x manifests itself as a change in the sign of paramagnetic Curie temperature for this composition as though magnetic coupling sensitively depends on such crystallographic distortions. All the compositions exhibit spin-glass anomalies with an unusually large frequency dependence of the peak temperature in susceptibility in a temperature range below 50 K, interestingly obeying Vogel-Fulcher relationship even for the stoichiometric compounds.

  14. Synthesis, crystal structure, IR, 1H NMR and theoretical calculations of 1,2,4-triazole Schiff base

    Science.gov (United States)

    Jin, R. Y.; Sun, X. H.; Liu, Y. F.; Long, W.; Lu, W. T.; Ma, H. X.

    2014-03-01

    5-Propyl-4-amino-1,2,4-triazole Schiff base was characterized by FT-IR, 1H NMR and X-ray single crystal diffraction techniques. The compound crystallizes in the triclinic space group p-1 with z = 2. The molecular geometry was optimized using density functional theory (DFT/B3LYP) and hartree fock (HF) methods with the 6-311G+(d,p) and 6-311G basis set in ground state. From the optimized geometry of the molecule, vibrational frequencies, HOMO-LUMO and natural bond orbital (NBO) were calculated with B3LYP/6-311G+(d,p) level. In addition, gauge independent atomic orbital (GIAO) 1H NMR chemical shift values was calculated at B3LYP/6-311G+(d,p) and HF/6-311G+(d,p) level.

  15. Endotoxin mediated-iNOS induction causes insulin resistance via ONOO⁻ induced tyrosine nitration of IRS-1 in skeletal muscle.

    Directory of Open Access Journals (Sweden)

    Geneviève Pilon

    Full Text Available BACKGROUND: It is believed that the endotoxin lipopolysaccharide (LPS is implicated in the metabolic perturbations associated with both sepsis and obesity (metabolic endotoxemia. Here we examined the role of inducible nitric oxide synthase (iNOS in skeletal muscle insulin resistance using LPS challenge in rats and mice as in vivo models of endotoxemia. METHODOLOGY/PRINCIPAL FINDINGS: Pharmacological (aminoguanidine and genetic strategies (iNOS⁻/⁻ mice were used to counter iNOS induction in vivo. In vitro studies using peroxynitrite (ONOO⁻ or inhibitors of the iNOS pathway, 1400 W and EGCG were conducted in L6 myocytes to determine the mechanism by which iNOS mediates LPS-dependent insulin resistance. In vivo, both pharmacological and genetic invalidation of iNOS prevented LPS-induced muscle insulin resistance. Inhibition of iNOS also prevented insulin resistance in myocytes exposed to cytokine/LPS while exposure of myocytes to ONOO⁻ fully reproduced the inhibitory effect of cytokine/LPS on both insulin-stimulated glucose uptake and PI3K activity. Importantly, LPS treatment in vivo and iNOS induction and ONOO⁻ treatment in vitro promoted tyrosine nitration of IRS-1 and reduced insulin-dependent tyrosine phosphorylation. CONCLUSIONS/SIGNIFICANCE: Our work demonstrates that iNOS-mediated tyrosine nitration of IRS-1 is a key mechanism of skeletal muscle insulin resistance in endotoxemia, and presents nitrosative modification of insulin signaling proteins as a novel therapeutic target for combating muscle insulin resistance in inflammatory settings.

  16. The transcriptional coactivators p/CIP and SRC-1 control insulin resistance through IRS1 in obesity models.

    Directory of Open Access Journals (Sweden)

    Zhiyong Wang

    Full Text Available Three p160 family members, p/CIP, SRC1, and TIF2, have been identified as transcriptional coactivators for nuclear hormone receptors and other transcription factors in vitro. In a previous study, we reported initial characterization of the obesity-resistant phenotypes of p/CIP and SRC-1 double knockout (DKO mice, which exhibit increased energy expenditure, and suggested that nuclear hormone receptor target genes were involved in these phenotypes. In this study, we demonstrate that p/CIP and SRC1 control insulin signaling in a cell-autonomous manner both in vitro and in vivo. Genetic deletion of p/CIP and SRC-1 increases glucose uptake and enhances insulin sensitivity in both regular chow- and high fat diet-fed DKO mice despite increased food intake. Interestingly, we discover that loss of p/CIP and SRC-1 results in resistance to age-related obesity and glucose intolerance. We show that expression levels of a key insulin signaling component, insulin receptor substrate 1 (IRS1, are significantly increased in two cell lines representing fat and muscle lineages with p/CIP and SRC-1 deletions and in white adipose tissue and skeletal muscle of DKO mice; this may account for increased glucose metabolism and insulin sensitivity. This is the first evidence that the p160 coactivators control insulin signaling and glucose metabolism through IRS1. Therefore, our studies indicate that p/CIP and SRC-1 are potential therapeutic targets not only for obesity but also for diabetes.

  17. A COMPARATIVE ASTROCHEMICAL STUDY OF THE HIGH-MASS PROTOSTELLAR OBJECTS NGC 7538 IRS 9 AND IRS 1

    Energy Technology Data Exchange (ETDEWEB)

    Barentine, John C.; Lacy, John H. [Department of Astronomy, University of Texas at Austin, 1 University Station, C1400, Austin, TX 78712-0259 (United States)

    2012-10-01

    We report the results of a spectroscopic study of the high-mass protostellar object NGC 7538 IRS 9 and compare our observations to published data on the nearby object NGC 7538 IRS 1. Both objects originated in the same molecular cloud and appear to be at different points in their evolutionary histories, offering an unusual opportunity to study the temporal evolution of envelope chemistry in objects sharing a presumably identical starting composition. Observations were made with the Texas Echelon Cross Echelle Spectrograph, a sensitive, high spectral resolution (R {lambda}/{Delta}{lambda} {approx_equal} 100,000) mid-infrared grating spectrometer. Forty-six individual lines in vibrational modes of the molecules C{sub 2}H{sub 2}, CH{sub 4}, HCN, NH{sub 3}, and CO were detected, including two isotopologues ({sup 13}CO, {sup 12}C{sup 18}O) and one combination mode ({nu}{sub 4} + {nu}{sub 5} C{sub 2}H{sub 2}). Fitting synthetic spectra to the data yielded the Doppler shift, excitation temperature, Doppler b parameter, column density, and covering factor for each molecule observed; we also computed column density upper limits for lines and species not detected, such as HNCO and OCS. We find differences among spectra of the two objects likely attributable to their differing radiation and thermal environments. Temperatures and column densities for the two objects are generally consistent, while the larger line widths toward IRS 9 result in less saturated lines than those toward IRS 1. Finally, we compute an upper limit on the size of the continuum-emitting region ({approx}2000 AU) and use this constraint and our spectroscopy results to construct a schematic model of IRS 9.

  18. 4PS/insulin receptor substrate (IRS)-2 is the alternative substrate of the insulin receptor in IRS-1-deficient mice.

    Science.gov (United States)

    Patti, M E; Sun, X J; Bruening, J C; Araki, E; Lipes, M A; White, M F; Kahn, C R

    1995-10-20

    Insulin receptor substrate-1 (IRS-1) is the major cytoplasmic substrate of the insulin and insulin-like growth factor (IGF)-1 receptors. Transgenic mice lacking IRS-1 are resistant to insulin and IGF-1, but exhibit significant residual insulin action which corresponds to the presence of an alternative high molecular weight substrate in liver and muscle. Recently, Sun et al. (Sun, X.-J., Wang, L.-M., Zhang, Y., Yenush, L. P., Myers, M. G., Jr., Glasheen, E., Lane, W.S., Pierce, J. H., and White, M. F. (1995) Nature 377, 173-177) purified and cloned 4PS, the major substrate of the IL-4 receptor-associated tyrosine kinase in myeloid cells, which has significant structural similarity to IRS-1. To determine if 4PS is the alternative substrate of the insulin receptor in IRS-1-deficient mice, we performed immunoprecipitation, immunoblotting, and phosphatidylinositol (PI) 3-kinase assays using specific antibodies to 4PS. Following insulin stimulation, 4PS is rapidly phosphorylated in liver and muscle, binds to the p85 subunit of PI 3-kinase, and activates the enzyme. Insulin stimulation also results in the association of 4PS with Grb 2 in both liver and muscle. In IRS-1-deficient mice, both the phosphorylation of 4PS and associated PI 3-kinase activity are enhanced, without an increase in protein expression. Immunodepletion of 4PS from liver and muscle homogenates removes most of the phosphotyrosine-associated PI 3-kinase activity in IRS-1-deficient mice. Thus, 4PS is the primary alternative substrate, i.e. IRS-2, which plays a major role in physiologic insulin signal transduction via both PI 3-kinase activation and Grb 2/Sos association. In IRS-1-deficient mice, 4PS/IRS-2 provides signal transduction to these two major pathways of insulin signaling.

  19. 宝腾GEN-2

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    宝腾 GEN-2是由马来西亚宝腾汽车公司和莲花工程公司(Lotus Engineering)耗时4年共同开发的,也是宝腾收购莲花后设计的第一款新车。车名GEN-2也就是 GENRERATION 2的缩写。在 GEN-2的开发过程中,造型设计和工程设计是由宝腾公司完成的,莲花工程公司对 GEN-2进行了底盘调校,莲花设计中心(Lotus DesignStudio)完成了内饰设计。发动机则是由宝腾汽车公司开发、莲花工程公司调校的名为 Campro(Campro 是

  20. FutureGen Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Cabe, Jim; Elliott, Mike

    2010-09-30

    This report summarizes the comprehensive siting, permitting, engineering, design, and costing activities completed by the FutureGen Industrial Alliance, the Department of Energy, and associated supporting subcontractors to develop a first of a kind near zero emissions integrated gasification combined cycle power plant and carbon capture and storage project (IGCC-CCS). With the goal to design, build, and reliably operate the first IGCC-CCS facility, FutureGen would have been the lowest emitting pulverized coal power plant in the world, while providing a timely and relevant basis for coal combustion power plants deploying carbon capture in the future. The content of this report summarizes key findings and results of applicable project evaluations; modeling, design, and engineering assessments; cost estimate reports; and schedule and risk mitigation from initiation of the FutureGen project through final flow sheet analyses including capital and operating reports completed under DOE award DE-FE0000587. This project report necessarily builds upon previously completed siting, design, and development work executed under DOE award DE-FC26- 06NT4207 which included the siting process; environmental permitting, compliance, and mitigation under the National Environmental Policy Act; and development of conceptual and design basis documentation for the FutureGen plant. For completeness, the report includes as attachments the siting and design basis documents, as well as the source documentation for the following: • Site evaluation and selection process and environmental characterization • Underground Injection Control (UIC) Permit Application including well design and subsurface modeling • FutureGen IGCC-CCS Design Basis Document • Process evaluations and technology selection via Illinois Clean Coal Review Board Technical Report • Process flow diagrams and heat/material balance for slurry-fed gasifier configuration • Process flow diagrams and heat/material balance

  1. Aerobic fitness testing in 6- to 9-year-old children: reliability and validity of a modified Yo–Yo IR1 test and the Andersen test

    DEFF Research Database (Denmark)

    Ahler, Thomas

    2011-01-01

    Målet med dette studie var at undersøge to indirekte VO2max tests (Yo–Yo IR1 og Andersen test) hos en gruppe 0 og 2. klasses børn mht. reproducerbarhed og mht. validitet i relation til en direkte målt VO2max. 35 skolelever i alderen 6-9 år blev inkluderet i studiet (18 fra 0., og 17 fra 2. klasse......). 32, 34 og 32 elever gennemførte hhv. Yo–Yo IR1 testen, Andersen testen, og en progressive løbebåndstest for at bestemme VO2max I alt gennemførte 26 elever alle 5 testrunder. Både Yo–Yo IR1 and Andersen testen er reproducerbare og er korreleret med VO2max hos skoleelever i alderen 6-9 år....

  2. Induction of miR-29a by saturated fatty acids impairs insulin signaling and glucose uptake through translational repression of IRS-1 in myocytes.

    Science.gov (United States)

    Yang, Won-Mo; Jeong, Hyo-Jin; Park, Seung-Yoon; Lee, Wan

    2014-06-13

    MicroRNAs have been shown to play an important role in insulin signaling but their biological function in insulin resistance induced by saturated fatty acids (SFA) remains largely unknown. Here, we report that SFA palmitate and high fat diet (HFD) significantly increase expression of miR-29a in myocytes. miR-29a targets IRS-1 3'UTR directly and represses IRS-1 expression at the translational level. Furthermore, the ectopic expression of miR-29a impairs insulin signaling and glucose uptake in myocytes through a substantial decrease in IRS-1. These findings suggest that the up-regulation of miR-29a by SFA is causally related to the development of insulin resistance in myocytes.

  3. Magnetic fluctuations driven insulator-to-metal transition in Ca(Ir1-xRux)O3

    Science.gov (United States)

    Gunasekera, J.; Harriger, L.; Dahal, A.; Heitmann, T.; Vignale, G.; Singh, D. K.

    2015-12-01

    Magnetic fluctuations in transition metal oxides are a subject of intensive research because of the key role they are expected to play in the transition from the Mott insulator to the unconventional metallic phase of these materials, and also as drivers of superconductivity. Despite much effort, a clear link between magnetic fluctuations and the insulator-to-metal transition has not yet been established. Here we report the discovery of a compelling link between magnetic fluctuations and the insulator-to-metal transition in Ca(Ir1-xRux)O3 perovskites as a function of the substitution coefficient x. We show that when the material turns from insulator to metal, at a critical value of x ~ 0.3, magnetic fluctuations tend to change their character from antiferromagnetic, a Mott insulator phase, to ferromagnetic, an itinerant electron state with Hund’s orbital coupling. These results are expected to have wide-ranging implications for our understanding of the unconventional properties of strongly correlated electrons systems.

  4. Magnetic fluctuations driven insulator-to-metal transition in Ca(Ir1−xRux)O3

    Science.gov (United States)

    Gunasekera, J.; Harriger, L.; Dahal, A.; Heitmann, T.; Vignale, G.; Singh, D. K.

    2015-01-01

    Magnetic fluctuations in transition metal oxides are a subject of intensive research because of the key role they are expected to play in the transition from the Mott insulator to the unconventional metallic phase of these materials, and also as drivers of superconductivity. Despite much effort, a clear link between magnetic fluctuations and the insulator-to-metal transition has not yet been established. Here we report the discovery of a compelling link between magnetic fluctuations and the insulator-to-metal transition in Ca(Ir1−xRux)O3 perovskites as a function of the substitution coefficient x. We show that when the material turns from insulator to metal, at a critical value of x ~ 0.3, magnetic fluctuations tend to change their character from antiferromagnetic, a Mott insulator phase, to ferromagnetic, an itinerant electron state with Hund’s orbital coupling. These results are expected to have wide-ranging implications for our understanding of the unconventional properties of strongly correlated electrons systems. PMID:26647965

  5. IRS1, TCF7L2, ADRB1, PPARG, and HHEX Polymorphisms Associated with Atherogenic Risk in Mexican Population

    Directory of Open Access Journals (Sweden)

    B. I. Estrada-Velasco

    2013-01-01

    Full Text Available Objective. We aimed to explore the association between polymorphisms of IRS1 (rs1801278, TCF7L2 (rs7903146 and rs12255372, ADRB1 (rs1801253, PPARG (rs1801282, and HHEX (rs5015480 genes with atherogenic risk (AI = Total cholesterol/HDL in MetS, T2D, and healthy populations from the Mexican Social Security Institute. Methodology and Results. Four hundred thirty-five MetS, 517 T2D, and 547 healthy individuals were selected. The association between the SNPs and the atherogenic index was evaluated by multiple linear regression and multinomial logistic regression models. The ADRB1 gene showed a statistically significant association with high-risk atherogenic index, OR=2.94 (IC 95% 1.64–5.24; P<0.0001 for the Arg/Gly variant, under the dominant model an OR=2.96 (IC 95% 1.67–5.25; P<0.0001, and under the Log additive model an OR=2.52 (IC 95% 1.54–4.15; P<0.0001. Conclusions. The Arg389Gly polymorphism of the ADRB1 gene may be a worthy biological marker to predict the risk of developing cardiovascular diseases given a high-risk atherogenic index.

  6. IRS1, TCF7L2, ADRB1, PPARG, and HHEX Polymorphisms Associated with Atherogenic Risk in Mexican Population

    Science.gov (United States)

    Estrada-Velasco, B. I.; Cruz, M.; Madrid-Marina, V.; Martínez-Nava, G. A.; Gomez-Zamudio, J.; Burguete-García, A. I.

    2013-01-01

    Objective. We aimed to explore the association between polymorphisms of IRS1 (rs1801278), TCF7L2 (rs7903146 and rs12255372), ADRB1 (rs1801253), PPARG (rs1801282), and HHEX (rs5015480) genes with atherogenic risk (AI = Total cholesterol/HDL) in MetS, T2D, and healthy populations from the Mexican Social Security Institute. Methodology and Results. Four hundred thirty-five MetS, 517 T2D, and 547 healthy individuals were selected. The association between the SNPs and the atherogenic index was evaluated by multiple linear regression and multinomial logistic regression models. The ADRB1 gene showed a statistically significant association with high-risk atherogenic index, OR = 2.94 (IC 95% 1.64–5.24; P < 0.0001) for the Arg/Gly variant, under the dominant model an OR = 2.96 (IC 95% 1.67–5.25; P < 0.0001), and under the Log additive model an OR = 2.52 (IC 95% 1.54–4.15; P < 0.0001). Conclusions. The Arg389Gly polymorphism of the ADRB1 gene may be a worthy biological marker to predict the risk of developing cardiovascular diseases given a high-risk atherogenic index. PMID:24371822

  7. Ab initio study of structural, electronic, and thermal properties of Ir_{1-x}Rh_{x} alloys

    Directory of Open Access Journals (Sweden)

    Sh. Ahmed

    2015-06-01

    Full Text Available The structural, electronic, mechanical and thermal properties of Ir_{1-x}Rh_{x} alloys was studied systematically using ab initio density functional theory at different concentration (x = 0.00, 0.25, 0.50, 0.75, 1.00. The Special Quasirandom Structure method was used to make the alloys with FCC structure with four atoms per unit cell. The ground state properties such as lattice constant and bulk modulus were calculated to find the equilibrium atomic position for stable alloys. The calculated ground state properties are in good agreement with the experimental and previously presented other theoretical data. The electronic band structure and density of states were calculated to study the electronic properties for these alloys at different concentration. The electronic properties substantiate metallic behavior of alloys. The first principle density functional perturbation theory as implemented in quasiharmonic approximation was used for the calculation of thermal properties. We have calculated the thermal properties such the Debye temperatures, vibration energy, entropy, constant-volume specific heat and internal energy. The ab initio linear-response method was used for phonon densities of states calculations.

  8. Application of IRS-1D data in water erosion features detection (case study: Nour roud catchment, Iran).

    Science.gov (United States)

    Solaimani, K; Amri, M A Hadian

    2008-08-01

    The aim of this study was capability of Indian Remote Sensing (IRS) data of 1D to detecting erosion features which were created from run-off. In this study, ability of PAN digital data of IRS-1D satellite was evaluated for extraction of erosion features in Nour-roud catchment located in Mazandaran province, Iran, using GIS techniques. Research method has based on supervised digital classification, using MLC algorithm and also visual interpretation, using PMU analysis and then these were evaluated and compared. Results indicated that opposite of digital classification, with overall accuracy 40.02% and kappa coefficient 31.35%, due to low spectral resolution; visual interpretation and classification, due to high spatial resolution (5.8 m), prepared classifying erosion features from this data, so that these features corresponded with the lithology, slope and hydrograph lines using GIS, so closely that one can consider their boundaries overlapped. Also field control showed that this data is relatively fit for using this method in investigation of erosion features and specially, can be applied to identify large erosion features.

  9. Inferring the Evolutionary Stages of the internal structures of NGC 7538 S and IRS1 from Chemistry

    CERN Document Server

    Feng, S; Semenov, D; Henning, Th; Linz, H; Mills, E A C; Teague, R

    2016-01-01

    To unambiguously diagnose the detailed physical mechanisms and the evolutionary status of high-mass star-forming regions (HMSFRs), we have performed 0.4" ($\\sim1000$ AU) resolution observations towards NGC7538S and IRS1, using the Plateau de Bure Interferometer (PdBI). This paper presents a joint analysis of the 1.37 mm continuum emission and the line intensity of 15 molecular species. Assuming local thermal equilibrium, we derived molecular column densities and molecular abundances for each internal gas substructure which is spatially resolved. These derived quantities are compared with a suite of 1-D gas-grain models. NGC7538S is resolved into at least three dense gas condensations. Despite the comparable continuum intensity of these condensations, their differing molecular line emission is suggestive of an overall chemical evolutionary trend from the northeast to the southeast. Since these condensations are embedded within the same parent gas core, their differing chemical properties are the most likely du...

  10. Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (IRS1) Is Dispensable for Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase.

    Science.gov (United States)

    Copps, Kyle D; Hançer, Nancy J; Qiu, Wei; White, Morris F

    2016-04-15

    Constitutive activation of the mammalian target of rapamycin complex 1 and S6 kinase (mTORC1→ S6K) attenuates insulin-stimulated Akt activity in certain tumors in part through "feedback" phosphorylation of the upstream insulin receptor substrate 1 (IRS1). However, the significance of this mechanism for regulating insulin sensitivity in normal tissue remains unclear. We investigated the function of Ser-302 in mouse IRS1, the major site of its phosphorylation by S6K in vitro, through genetic knock-in of a serine-to-alanine mutation (A302). Although insulin rapidly stimulated feedback phosphorylation of Ser-302 in mouse liver and muscle, homozygous A302 mice (A/A) and their knock-in controls (S/S) exhibited similar glucose homeostasis and muscle insulin signaling. Furthermore, both A302 and control primary hepatocytes from which Irs2 was deleted showed marked inhibition of insulin-stimulated IRS1 tyrosine phosphorylation and PI3K binding after emetine treatment to raise intracellular amino acids and activate mTORC1 → S6K signaling. To specifically activate mTORC1 in mouse tissue, we deleted hepatic Tsc1 using Cre adenovirus. Although it moderately decreased IRS1/PI3K association and Akt phosphorylation in liver, Tsc1 deletion failed to cause glucose intolerance or promote hyperinsulinemia in mixed background A/A or S/S mice. Moreover, Tsc1 deletion failed to stimulate phospho-Ser-302 or other putative S6K sites within IRS1, whereas ribosomal S6 protein was constitutively phosphorylated. Following acute Tsc1 deletion from hepatocytes, Akt phosphorylation, but not IRS1/PI3K association, was rapidly restored by treatment with the mTORC1 inhibitor rapamycin. Thus, within the hepatic compartment, mTORC1 → S6K signaling regulates Akt largely through IRS-independent means with little effect upon physiologic insulin sensitivity.

  11. Summary of CPAS Gen II Parachute Analysis

    Science.gov (United States)

    Morris, Aaron L.; Bledsoe, Kristin J.; Fraire, Usbaldo, Jr.; Moore, James W.; Olson, Leah M.; Ray, Eric

    2011-01-01

    The Orion spacecraft is currently under development by NASA and Lockheed Martin. Like Apollo, Orion will use a series of parachutes to slow its descent and splashdown safely. The Orion parachute system, known as the CEV Parachute Assembly System (CPAS), is being designed by NASA, the Engineering and Science Contract Group (ESCG), and Airborne Systems. The first generation (Gen I) of CPAS testing consisted of thirteen tests and was executed in the 2007-2008 timeframe. The Gen I tests provided an initial understanding of the CPAS parachutes. Knowledge gained from Gen I testing was used to plan the second generation of testing (Gen II). Gen II consisted of six tests: three singleparachute tests, designated as Main Development Tests, and three Cluster Development Tests. Gen II required a more thorough investigation into parachute performance than Gen I. Higher fidelity instrumentation, enhanced analysis methods and tools, and advanced test techniques were developed. The results of the Gen II test series are being incorporated into the CPAS design. Further testing and refinement of the design and model of parachute performance will occur during the upcoming third generation of testing (Gen III). This paper will provide an overview of the developments in CPAS analysis following the end of Gen I, including descriptions of new tools and techniques as well as overviews of the Gen II tests.

  12. GenLab, Laboratorio Virtual de Genética

    Directory of Open Access Journals (Sweden)

    García Sergio

    2000-12-01

    Full Text Available

    GenLab es el nombre que tiene el software diseñado por nosotros, en el cual se modela el proceso meiótico y la fecundación en organismos diploides. El objetivo de esta aplicación es ilustrar el resultado de un cruce determinado, tratando de ser lo más ajustados a la realidad. La modelación de la reproducción sexual se realiza internamente y el GenLab se limita a presentar los resultados según el número de descendencia seleccionado para un cruce específico, esto significa que se puede escoger una gran cantidad de características para los parentales y se puede estudiar la frecuencia de estos en la descendencia. El modelo cuenta con base de datos donde están almacenados algunos de los locus de Drosophila melanogaster junto con su ubicación en centimorgans 1. EI propósito de este modelo es servir como herramienta pedagógica  y didáctica tanto en universidades como en colegios, facilitando el aprendizaje de algunos principios básicos de la genética, por lo cual puede ser usado si se cuenta con una conexión a Internet y un navegador visitando http://biologia.unal.edu.co/fidel.

  13. TrafficGen Architecture Document

    Science.gov (United States)

    2016-01-01

    Poisson, Jitter, and Clone . Researchers can experience an added dimension of network traffic visualization by pairing TrafficGen with the NRL Scripted...MVC Classes The top-level MVC classes control the workspace of the application, specifically the menu and the way the nodes and events are rendered...This is a container class that holds singleton instances of the view classes employed by this application. Because of the way this application is

  14. Aerobic fitness testing in 6- to 9-year-old children: reliability and validity of a modified Yo-Yo IR1 test and the Andersen test

    DEFF Research Database (Denmark)

    Ahler, T; Bendiksen, Mads; Krustrup, Peter

    2012-01-01

    This study analysed the reliability and validity of two intermittent running tests (the Yo-Yo IR1 test and the Andersen test) as tools for estimating VO(2max) in children under the age of 10. Two groups, aged 6-7 years (grade 0, n = 18) and 8-9 years (grade 2, n = 16), carried out two repetitions...... of a modified Yo-Yo IR1 test (2 × 16 m) and the Andersen test, as well as an incremental treadmill test, to directly determine the VO(2max). No significant differences were observed in test-retest performance of the Yo-Yo IR1 test [693 ± 418 (±SD) and 670 ± 328 m, r (2) = 0.79, CV = 19%, p > 0.05, n = 32......) and the Andersen test (988 ± 77 and 989 ± 87 m, r (2) = 0.86, CV = 3%, p > 0.05, n = 31). The Yo-Yo IR1 (r (2) = 0.47, n = 31, p ...

  15. Muscle-Specific IRS-1 Ser→Ala Transgenic Mice Are Protected From Fat-Induced Insulin Resistance in Skeletal Muscle

    National Research Council Canada - National Science Library

    Katsutaro Morino; Susanne Neschen; Stefan Bilz; Saki Sono; Dimitrios Tsirigotis; Richard M. Reznick; Irene Moore; Yoshio Nagai; Varman Samuel; David Sebastian; Morris White; William Philbrick; Gerald I. Shulman

    2008-01-01

    Muscle-Specific IRS-1 Ser→Ala Transgenic Mice Are Protected From Fat-Induced Insulin Resistance in Skeletal Muscle Katsutaro Morino 1 2 , Susanne Neschen 1 2 , Stefan Bilz 2 , Saki Sono 2 , Dimitrios Tsirigotis 2 3 , Richard M...

  16. 体重指数与PCOS患者子宫内膜IRS-1、GLUT4表达水平的相关性

    Institute of Scientific and Technical Information of China (English)

    张小魏; 王晓燕; 王丽娥; 彭真; 邹淑花

    2016-01-01

    目的:探讨PCOS患者体重指数(BMI)与子宫内膜IRS-1、GLUT4等表达的相关性.方法:选取在本院就诊的PCOS患者60例,按BMI分为3组:<25kg/m2组(A组,20例),25kg/m2≤BMI≤30kg/m2组(B组,25例),>30kg/m2组(C组,15例).选取20例输卵管性因素不孕患者为对照组.于卵泡期刮取内膜,免疫组化染色和Western blot法检测子宫内膜IRS-1、GLUT4表达.结果:BMI高值组的IRS-1、GLUT4表达明显低于BMI低值组.BMI水平与子宫内膜IRS-1、GLUT4呈负相关(P<0.05).结论:升高的BMI可能是PCOS患者子宫内膜发生胰岛素抵抗的危险因素之一.

  17. SOCS-3 is involved in the downregulation of the acute insulin-like effects of growth hormone in rat adipocytes by inhibition of Jak2/IRS-1 signaling

    DEFF Research Database (Denmark)

    Ridderstråle, M; Amstrup, J; Hilton, D J;

    2003-01-01

    identified family of suppressors of cytokine signaling (SOCS) proteins in the transition between the refractory and the responsive states in rat adipocytes. The ability of GH to stimulate lipogenesis and tyrosine phosphorylation of the GH receptor (GHR), Janus kinase 2 (Jak2), insulin receptor substrate-1...... (IRS-1) and -2 (IRS-2) was greatly reduced in refractory as compared to responsive primary rat adipocytes. However, phosphorylation of Signal Transducer and Activator of Transcription 5 (Stat5) was not affected. SOCS-3 and CIS mRNA levels were significantly higher in refractory compared to responsive...... cells and could be induced by GH, whereas the level of SOCS-2 mRNA was unchanged. With overexpression of GHR, Jak2 and IRS-1 along with each of these SOCS proteins in human A293 cells, we could demonstrate that both SOCS-1 and SOCS-3 completely inhibited the GH-stimulated tyrosine phosphorylation of IRS...

  18. The use of Yo-Yo IR1 and Andersen testing for fitness and maximal heart rate assessments of 6-10 yr old school children

    DEFF Research Database (Denmark)

    Ahler, Thomas

    2012-01-01

    We evaluated a sub-maximal and maximal version of the Yo-Yo IR1 childrens test (YYIR1C) and the Andersen test for fitness and maximal HR assessments of children aged 6-10. Two repetitions of the YYIR1C and Andersen tests were carried out within one week by 6-7 and 8-9 year olds (grade 0, n=17...

  19. Induction of miR-96 by Dietary Saturated Fatty Acids Exacerbates Hepatic Insulin Resistance through the Suppression of INSR and IRS-1.

    Science.gov (United States)

    Yang, Won-Mo; Min, Kyung-Ho; Lee, Wan

    2016-01-01

    Obesity is defined as the excessive accumulation of body fat that ultimately leads to chronic metabolic diseases. Diets rich in saturated fatty acids (SFA) exacerbate obesity and hepatic steatosis, which increase the risk of hepatic insulin resistance and type 2 diabetes (T2DM). Although microRNAs (miRNAs) play an important role in a range of biological processes, the implications of SFA-induced miRNAs in metabolic dysregulation, particularly in the pathogenesis of hepatic insulin resistance, are not well understood. This study investigated the implications of miR-96, which is induced strongly by SFA, in the development of hepatic insulin resistance. The liver of HFD mice and the palmitate-treated hepatocytes exhibited an impairment of insulin signaling due to the significant decrease in INSR and IRS-1 expression. According to expression profiling and qRT-PCR analysis of the miRNAs, the expression level of miR-96 was higher in hepatocytes treated with palmitate. Moreover, miR-96 was also upregulated in the liver of HFD mice. Interestingly, miR-96 targeted the 3'UTRs of INSR and IRS-1 directly, and repressed the expression of INSR and IRS-1 at the post-transcriptional level. Accordingly, the overexpression of miR-96 was found to cause a significant decrease in INSR and IRS-1 expression, thereby leading to an impairment of insulin signaling and glycogen synthesis in hepatocytes. These results reveal a novel mechanism whereby miR-96 promotes the pathogenesis of hepatic insulin resistance resulted from SFA or obesity.

  20. Coffee extract inhibits adipogenesis in 3T3-L1 preadipocyes by interrupting insulin signaling through the downregulation of IRS1.

    Science.gov (United States)

    Maki, Chihiro; Funakoshi-Tago, Megumi; Aoyagi, Ryohei; Ueda, Fumihito; Kimura, Masaki; Kobata, Kenji; Tago, Kenji; Tamura, Hiroomi

    2017-01-01

    Although epidemiological data have indicated that a strong negative association exists between coffee consumption and the prevalence of obesity-associated diseases, the molecular mechanisms by which coffee intake prevents obesity-associated diseases has not yet been elucidated. In this study, we found that coffee intake significantly suppressed high-fat diet (HFD)-induced metabolic alternations such as increases in body weight and the accumulation of adipose tissue, and up-regulation of glucose, free fatty acid, total cholesterol and insulin levels in the blood. We also found that coffee extract significantly inhibited adipogenesis in 3T3-L1 preadipocytes. In the early phase of adipogenesis, 3T3-L1 cells treated with coffee extract displayed the retardation of cell cycle entry into the G2/M phase called as mitotic clonal expansion (MCE). Coffee extract also inhibited the activation of CCAAT/enhancer-binding protein β (C/EBPβ) by preventing its phosphorylation by ERK. Furthermore, the coffee extract suppressed the adipogenesis-related events such as MCE and C/EBPβ activation through the down-regulation of insulin receptor substrate 1 (IRS1). The stability of the IRS1 protein was markedly decreased by the treatment with coffee extract due to proteasomal degradation. These results have revealed an anti-adipogenic function for coffee intake and identified IRS1 as a novel target for coffee extract in adipogenesis.

  1. Acute effects of Yo-Yo intermittent recovery test level 1 (Yo-YoIR1) on hemorheological parameters in female volleyball players.

    Science.gov (United States)

    Kilic-Toprak, Emine; Yapici, Ayşegül; Kilic-Erkek, Ozgen; Koklu, Yusuf; Tekin, Volkan; Alemdaroglu, Utku; Bor-Kucukatay, Melek

    2015-07-16

    In the present study, we investigated possible alterations in red blood cell (RBC) deformability, plasma and whole blood viscosities (WBV) and hematological parameters in response to Yo-Yo intermittent recovery test level 1 (Yo-YoIR1) which is currently used to assess endurance performance, in female volleyball players. Eight volleyball player volunteers from Pamukkale University (mean age19,9 ± 2,2 years; mean body height 177.5 ± 1.99 cm; mean body mass index 21.66 ± 0.64 kg/m2) participated to the study. Blood samples were collected before and immediately after test. Red blood cell (RBC) deformability was determined by ektacytometer, plasma and whole blood viscosities (WBV) by a cone-plate rotational viscometer. Hematological parameters were determined using an electronic hematology analyzer. The Yo-YoIR1 applied, induced acute increments in WBV at native hematocrit (Hct) measured at a shear rate of 150 s-1 and 375 s-1, RBC deformability and WBC count. The results of the current study indicate that, the Yo-Yo IR1 test used to determine physical capacity of the player, by resulting in increments in RBC deformability contributes blood flow and thus, athletic performance of the individual.

  2. The influence of coating solution and calcination condition on the durability of Ir1-xSnxO2/Ti anodes for oxygen evolution

    Science.gov (United States)

    Kato, Zenta; Kashima, Ryo; Tatsumi, Kohei; Fukuyama, Shinnosuke; Izumiya, Koichi; Kumagai, Naokazu; Hashimoto, Koji

    2016-12-01

    For oxygen formation without forming chlorine in seawater electrolysis for hydrogen production we have been using the anode consisting of three layers of MnO2-type multiple oxide catalyst, intermediate layer and titanium substrate. The intermediate layer was used for prevention of oxidation of the titanium substrate during anodic polarization for oxygen evolution and was prepared by calcination of butanol solutions of H2IrCl6 and SnCl4 coated on titanium. The protectiveness of Ir1-xSnxO2 layer formed was directly examined using Ir1-xSnxO2/Ti anodes in H2SO4 solution changing the preparation conditions of the layer. When the sum of Ir4+ and Sn4+ was 0.1 M, the highest protectiveness was observed at 0.06 M Sn4+. Although an increase in calcination temperature led to the formation of Ir1-x-ySnxTiyO2 triple oxide with a slightly lower catalytic activity for oxygen evolution, the anode calcined at 450 °C showed the highest protectiveness.

  3. A study of the region of massive star formation L379IRS1 in radio lines of methanol and other molecules

    Science.gov (United States)

    Kalenskii, S. V.; Shchurov, M. A.

    2016-04-01

    The results of spectral observations of the region of massive star formation L379IRS1 (IRAS18265-1517) are presented. The observations were carried out with the 30-m Pico Veleta radio telescope (Spain) at seven frequencies in the 1-mm, 2-mm, and 3-mm wavelength bands. Lines of 24 molecules were detected, from simple diatomic or triatomic species to complex eight- or nine-atom compounds such as CH3OCHO or CH3OCH3. Rotation diagrams constructed from methanol andmethyl cyanide lines were used to determine the temperature of the quiescent gas in this region, which is about 40-50 K. In addition to this warm gas, there is a hot component that is revealed through high-energy lines of methanol and methyl cyanide, molecular lines arising in hot regions, and the presence of H2O masers and Class II methanol masers at 6.7 GHz, which are also related to hot gas. One of the hot regions is probably a compact hot core, which is located near the southern submillimeter peak and is related to a group of methanol masers at 6.7 GHz. High-excitation lines at other positions may be associated with other hot cores or hot post-shock gas in the lobes of bipolar outflows. The rotation diagrams can be use to determine the column densities and abundances of methanol (10-9) and methyl cyanide (about 10-11) in the quiescent gas. The column densities of A- and E-methanol in L379IRS1 are essentually the same. The column densities of other observedmolecules were calculated assuming that the ratios of the molecular level abundances correspond to a temperature of 40 K. The molecular composition of the quiescent gas is close to that in another region of massive star formation, DR21(OH). The only appreciable difference is that the column density of SO2 in L379IRS1 is at least a factor of 20 lower than the value in DR21(OH). The SO2/CS and SO2/OCS abundance ratios, which can be used as chemical clocks, are lower in L379IRS1 than in DR21(OH), suggesting that L379IRS1 is probably younger than DR21(OH).

  4. Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.

    Science.gov (United States)

    Ezaki, T; Kawamura, Y; Li, N; Li, Z Y; Zhao, L; Shu, S

    2001-07-01

    Members of genus Peptostreptococcus have previously been found to be distantly related to the type species, Peptostreptococcus anaerobius, on the basis of 16S rDNA sequence similarities. They were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. The reclassification of the species of these three groups into three new genera, Peptoniphilus gen. nov., Anaerococcus gen. nov. and Gallicola gen. nov., is proposed. The genus Peptoniphilus gen. nov. includes the following butyrate-producing, non-saccharolytic species that use peptone and amino acids as major energy sources: Peptoniphilus asaccharolyticus comb. nov. (type species), Peptoniphilus lacrimaris comb. nov., Peptoniphilus harei comb. nov., Peptoniphilus indolicus comb. nov. and Peptoniphilus ivorii comb. nov. The genus Anaerococcus gen. nov. contains the saccharolytic, butyrate-producing species Anaerococcus prevotii comb. nov. (type species), Anaerococcus tetradius comb. nov., Anaerococcus lactolyticus comb. nov., Anaerococcus hydrogenalis comb. nov., Anaerococcus vaginalis comb. nov. and Anaerococcus octavius sp. nov. The genus Gallicola gen. nov. contains a single species, Gallicola barnesae comb. nov.

  5. Single-nucleotide polymorphism of INS, INSR, IRS1, IRS2, PPAR-G and CAPN10 genes in the pathogenesis of polycystic ovary syndrome

    Indian Academy of Sciences (India)

    MAHESWARI THANGAVELU; USHA RANI GODLA; SOLOMON F. D. PAUL; RAVI MADDALY

    2017-03-01

    Polycystic ovary syndrome (PCOS) is the most common and a complex female endocrine disorder, and is one of the leading cause of female infertility. Here, we aimed to investigate the association of single-nucleotide polymorphism of INS, INSR, IRS1, IRS2, PPAR-G and CAPN10 gene in the pathogenesis of PCOS. A hospital-based, observational case–control study was carried on 169 PCOS and 169 control women in the southern region of India. Genotype was carried out by real-time polymerase chain reaction. A chi-square (χ2) test was performed and the genotypes were verified to comply with the Hardy–Weinberg equilibrium. Odds ratio and 95% confidence interval were calculated to assess the relative risk. Comparison of clinical characteristics of women with PCOS and controls reveal an increase in body mass index (BMI), luteinizing hormone / follicle stimulating hormone (LH/FSH) ratio, glucose levels, insulin, testosterone, hirsutism and antral follicular count in PCOS women. The variant rs1801278 (P = 0.002; OR = 2.88; 95% CI = 1.43, 5.80) show an association with PCOS. In thegenotypic (P = 0.0002) and allelic models (P = 0.000), significance persisted even after Bonferroni correction. The genotypes of SNPs strongly influence BMI, LH, LH/FSH ratio, ovarian volume and antral follicular count in PCOS women. The study results were suggestive of a positive association between Gly972Arg of IRS1 and PCOS in the south Indian population, while INS, IRS2, PPAR-G and CAPN10 failed to show any association with PCOS in our studied population. Further studies focussing the role of IRS1 are warranted to delineate its implication towards PCOS.

  6. Over-expression of NYGGF4 (PID1) inhibits glucose transport in skeletal myotubes by blocking the IRS1/PI3K/AKT insulin pathway.

    Science.gov (United States)

    Wu, W L; Gan, W H; Tong, M L; Li, X L; Dai, J Z; Zhang, C M; Guo, X R

    2011-03-01

    Defects in insulin-stimulated glucose uptake in muscle are the important early events in the pathogenesis of insulin resistance. NYGGF4 (also named PID1) is a recently discovered gene which is suggested to be associated with obesity-associated insulin resistance. In this study, we aimed to investigate the effects of NYGGF4 on glucose uptake and insulin signaling in rat skeletal muscle cells. Rat L6 myoblasts were transfected with either an empty vector or an NYGGF4-expressing vector and induced to differentiate into mature L6 skeletal myotubes. Glucose uptake was determined by measuring uptake of 2-deoxy-d-[(3)H] glucose. Immunoblotting was performed to detect the translocation of insulin-sensitive glucose transporter 4 (GLUT4). Immunoblotting was also used to measure phosphorylation and total protein levels of the insulin signaling proteins including insulin receptor (IR), insulin receptor substrate 1 (IRS1), Akt, extracellular signal-regulated kinase 1 and 2 (ERK1/2), p38, and c-Jun-N-terminal kinase (JNK). NYGGF4 over-expression in L6 skeletal myotubes reduced insulin-stimulated glucose uptake and impaired insulin-stimulated GLUT4 translocation. It also diminished insulin-stimulated tyrosine phosphorylation of IRS1 and serine phosphorylation of Akt without affecting the phosphorylation of IR, ERK1/2, p38, or JNK. Over-expression of NYGGF4 inhibits glucose transport in skeletal myotubes by blocking the IRS1/PI3K/AKT insulin pathway. These observations highlight the potential role of NYGGF4 in glucose homeostasis and the development of insulin resistance in obesity. Copyright © 2010 Elsevier Inc. All rights reserved.

  7. Teleport Generation 3 (Teleport Gen 3)

    Science.gov (United States)

    2016-03-01

    8596 DSN Fax: Date Assigned: September 4, 2014 Program Information Program Name Teleport Generation 3 (Teleport Gen 3) DoD Component DoD The...2015 Approved APB Component Acquisition Executive (CAE) Approved Acquisition Program Baseline (APB) dated June 15, 2015 Teleport Gen 3 2016 MAR...System Network (DISN). The DoD Teleport upgrades selected sites from the Standardized Tactical Entry Point (STEP) program, which only provides reach

  8. Design of inspection and acceptance test methodology for TIG welded aluminum-alloy bracket for camera housings for IRS-1A space craft and executing it

    Science.gov (United States)

    Manglik, V. K.; Vaghmare, Rajeev; Shah, A. K.

    1992-10-01

    The Indian Remote Sensing Satellite (IRS) 1A was the first indigenously developed operational remote sensing satellite. The most critical element in the satellite was the remote sensing camera. The camera was mounted on aluminum alloy bracket which was fabricated by TIG welding. The methodology of acceptance and inspection of the TIG welded bracket is presented and discussed. These efforts not only provided the confidence in reliable welded joint but also provided trouble free operation of the camera on board the satellite for its whole life.

  9. The type 2 diabetes and insulin-resistance locus near IRS1 is a determinant of HDL cholesterol and triglycerides levels among diabetic subjects.

    Science.gov (United States)

    Sharma, Rajani; Prudente, Sabrina; Andreozzi, Francesco; Powers, Christine; Mannino, Gaia; Bacci, Simonetta; Gervino, Ernest V; Hauser, Thomas H; Succurro, Elena; Mercuri, Luana; Goheen, Elizabeth H; Shah, Hetal; Trischitta, Vincenzo; Sesti, Giorgio; Doria, Alessandro

    2011-05-01

    SNP rs2943641 near the insulin receptor substrate 1 (IRS1) gene has been found to be associated with type 2 diabetes (T2D) and insulin-resistance in genome-wide association studies. We investigated whether this SNP is associated with cardiovascular risk factors and coronary artery disease (CAD) among diabetic individuals. SNP rs2943641 was typed in 2133 White T2D subjects and tested for association with BMI, serum HDL cholesterol and triglycerides, hypertension history, and CAD risk. HDL cholesterol decreased by 1mg/dl (p = 0.004) and serum triglycerides increased by 6 mg/dl (p = 0.016) for each copy of the insulin-resistance allele. Despite these effects, no association was found with increased CAD risk (OR = 1.00, 95% CI 0.88-1.13). The insulin-resistance and T2D locus near the IRS1 gene is a determinant of lower HDL cholesterol among T2D subjects. However, this effect is small and does not translate into a detectable increase in CAD risk in this population. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  10. Debroyerella gen. nov. and Ulladulla gen. nov., two new lysianassoid genera (Crustacea, Amphipoda, Lysianassoidea).

    Science.gov (United States)

    Lowry, J K; Kilgallen, N M

    2015-02-19

    Two new genera and a new species of lysianassoid amphipods are described. Debroyerella gen. nov. is described for three Antarctic species previously assigned to the genus Cheirimedon. Ulladulla gen. nov. is described to accommodate the new species U. selje, from Australian waters. Diagnostic descriptions are given for the genera and all species are described in full.

  11. Unleashing Gen Y: Marketing Mars to Millennials

    Science.gov (United States)

    Leahy, Bart D.; Hidalgo, Loretta; Kloberdanz, Cassie

    2007-01-01

    Space advocates need to engage Generation Y (born 1977-1999).This outreach is necessary to recruit the next generation of scientists and engineers to explore Mars. Space advocates in the non-profit, private, and government sectors need to use a combination of technical communication, marketing, and politics, to develop messages that resonate with Gen Y. Until now, space messages have been generated by and for college-educated white males; Gen Y is much more diverse, including as much as one third minorities. Young women, too, need to be reached. My research has shown that messages emphasizing technology, fun, humor, and opportunity are the best means of reaching the Gen Y audience of 60 million (US population is 300 million). The important things space advocates must avoid are talking down to this generation, making false promises, or expecting them to "wait their turn" before they can participate. This is the MTV generation! We need to find ways of engaging Gen Y now to build a future where human beings can live and work on the planet Mars. In addition to the messages themselves, advocates need to keep up with Gen Y' s social networking and use of iPods, cell phones, and the Internet. NASA and space advocacy groups can use these tools for "viral marketing," where young people share targeted space-related information via cell phones or the Internet because they like it. Overall, Gen Y is a socially dynamic and media-savvy group; advocates' space messages need to be sincere, creative, and placed in locations where Gen Y lives. Mars messages must be memorable!

  12. Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov.

    Science.gov (United States)

    Kurtzman, Cletus P; Robnett, Christie J

    2014-05-01

    Three new non-ascosporic, ascomycetous yeast genera are proposed based on their isolation from currently described species and genera. Phylogenetic placement of the genera was determined from analysis of nuclear gene sequences for D1/D2 large subunit rRNA, small subunit rRNA, translation elongation factor-1α and RNA polymerase II, subunits B1 and B2. The new taxa are: Deakozyma gen. nov., type species Deakozyma indianensis sp. nov. (type strain NRRL YB-1937, CBS 12903); Danielozyma gen. nov., type species Danielozyma ontarioensis comb. nov. (type strain NRRL YB-1246, CBS 8502); D. litseae comb. nov. (type strain NRRL YB-3246, CBS 8799); Middelhovenomyces gen. nov., type species Middelhovenomyces tepae comb. nov. (type strain NRRL Y-17670, CBS 5115) and M. petrohuensis comb. nov. (type strain NRRL Y-17663, CBS 8173).

  13. ISOLASI DAN ANALISIS GEN HORMON PERTUMBUHAN LELE (Clarias gariepinus Burch.

    Directory of Open Access Journals (Sweden)

    Ibnu Dwi Buwono

    2012-12-01

    CAGGGTGCAGTTGGAATCC-3’ dapat mengkopi sekuen gen GH lele dengan ukuran fragmen PCR sekitar 1.400 bp. Sementara amplikon gen GH American catfish (Rhamdia quelen menggunakan primer Amc-GH-F dan Amc-GH-R sebesar 1.465 bp. Hasil analisis sekuensing gen penyandi GH menggunakan program BlastP dan Genetyx versi 7.0, menunjukkan bahwa sekuen gen penyandi GH lele dumbo memiliki homologi 80% dengan sekuen GH C. gariepinus pada bank gen (no. aksesi AF 416488.1, sehingga sebagian besar sekuen gen penyandi hormon pertumbuhan ikan tersebut dapat diamplifikasi secara in vitro.

  14. APP5肽类似物P165和罗格列酮对SH-SY5Y细胞IRS-1和p-CREB表达的影响%Effects of P165 and rosiglitazone on the expression of IRS-1 and p-CREB in SH-SY5Y cell

    Institute of Scientific and Technical Information of China (English)

    张景燕; 王蓉; 赵静姝; 姬志娟; 赵志炜; 盛树力

    2011-01-01

    观察APP5肽类似物P165和罗格列酮(rosiglitazone)对链脲佐菌素(streptozotocin,STZ)损伤人神经母细胞瘤株SH - SY5Y细胞后,胰岛素信号通路相关因子IRS-1和p-CREB表达水平的影响.方法将人神经母细胞瘤株SH-SY5Y细胞分为对照组、STZ损伤模型组(STZ 0.8mmol/L)、罗格列酮保护组(STZ 0.8 mmol/L+罗格列酮20 μmol/L),APP5肽类似物P165保护组(STZ 0.8 mmol/L+P165 30 μmol/L),进行细胞形态观察,IRS-1和p-CREB免疫细胞荧光染色观察,采用image-pro plus 图像分析软件测定平均吸光度值.结果与对照组相比,STZ损伤组细胞生长分裂减慢、突起缩短,细胞胞体皱缩,IRS-1和p-CREB免疫荧光染色强度减弱;与STZ损伤组相比,罗格列酮组和P165组突起伸展,细胞形态和细胞胞体皱缩现象明显改善,细胞出现强荧光染色.平均吸光度值测定结果显示:与对照组相比,STZ损伤组IRS-1和p-CREB平均吸光度值降低(P<0.008),与STZ损伤组相比,P165组和罗格列酮组平均吸光度值有所上升,差异均有统计学意义(P<0.008).结论APP5肽类似物P165和罗格列酮可能通过增加IRS-1、p-CREB的表达来改善STZ对于SH-SY5Y细胞的损伤,从而达到保护神经元的作用.

  15. Effect of S-equol on Insulin Sensibility and IRS-1 Expression of HepG2 Exposed to High Glucose%S-Equol对高糖培养HepG2细胞胰岛素敏感性及IRS-1表达的影响

    Institute of Scientific and Technical Information of China (English)

    刘凯; 陈卡; 周蕊; 卢宗亮; 易龙; 张婷; 糜漫天

    2013-01-01

    Objective: To investigate the effect of S-Equol on insulin sensibility and IRS-1 expression of HepG2 exposed to high glucose and the possible molecular mechanisms. Methods: HepG2 cells were cultured in high glucose in the absence or presence 1,10, 100 μM S-Eq. Effect of S-Eq on viability of cells was detected by MTT assay. The insulin-stimulated glycogen content of cells was evaluated by anthrone-sulfuric colorimetry. The mRNA and protein expression levels of IRS-1 were estimated by Real-time PCR and Western blot analysis, respectively. Results: There was no significant influence on viability of HepG2 cells by S-Eq. But the insulin sensibility was significantly increased by S-Eq and insulin-stimulated glycogen content was up-regulated by S-Eq at the concentration of 10u,M(P>0.01). We also found S-Eq significantly raised the levels of IRS-1 mRNA and protein. Conclusion: S-Eq can effectively improve insulin sensibility of HepG2 cells exposed to high glucose by regulating IRS-1 and this may be the critical theoretical basis of anti-diabetic effect of S-Eq.%目的:研究S型雌马酚(S-Equol,S-Eq)对高糖培养HepG2人肝癌细胞株胰岛素敏感性和胰岛素受体底物(insulin receptor substrate,IRS)-1表达的影响并探讨其可能的分子机制.方法:高糖培养HepG2细胞,1、10、100 μM S-Eq处理细胞后,MTT法检测细胞活力,硫酸蒽酮比色法检测胰岛素刺激细胞糖原合成量,Realtime PCR和Western blot法分别检测IRS-I mRNA及蛋白表达变化.结果:S-Eq对HepG2细胞活力无明显影响,但显著改善高糖培养条件下HepG2细胞胰岛素敏感性,其中10 μM S-Eq+H组胰岛素刺激后细胞糖原合成量上升最为显著(P<0.01),同时发现,S-Eq能显著上调IRS-1 mRNA和蛋白表达量.结论:S-Eq可能通过调控IRS-1的表达,增强高糖培养HepG2细胞胰岛素敏感性,这可能是S-Eq发挥其抗糖尿病作用的重要理论依据.

  16. siRNA-based gene silencing reveals specialized roles of IRS-1/Akt2 and IRS-2/Akt1 in glucose and lipid metabolism in human skeletal muscle.

    Science.gov (United States)

    Bouzakri, Karim; Zachrisson, Anna; Al-Khalili, Lubna; Zhang, Bei B; Koistinen, Heikki A; Krook, Anna; Zierath, Juleen R

    2006-07-01

    Type 2 diabetes is associated with defects in insulin signaling and the resulting abnormal glucose and lipid metabolism. The complexity of insulin signaling cascades is highlighted by the existence of multiple isoforms of target proteins implicated in metabolic and gene-regulatory events. We utilized siRNA to decipher the specific role of predominant insulin receptor substrates and Akt isoforms expressed in human skeletal muscle. Gene silencing revealed specialized roles of insulin signaling cascades to metabolic endpoints. IRS-1 and Akt2 were required for myoblast differentiation and glucose metabolism, whereas IRS-2 and Akt1 were dispensable. A key role of IRS-2 and Akt1 in lipid metabolism was revealed, highlighting reciprocal relationships between metabolic pathways. Unraveling the isoform-specific regulation of glucose and lipid metabolism by key elements along insulin signaling cascades through siRNA-mediated gene silencing in human tissues will facilitate the discovery of novel targets for the treatment of diabetes and related metabolic disorders.

  17. SOCS3 and IRS-1 gene expression differs between genotype 1 and genotype 2 hepatitis C virus-infected HepG2 cells.

    Science.gov (United States)

    Persico, Marcello; Russo, Roberta; Persico, Eliana; Svelto, Monica; Spano, Daniela; Andolfo, Immacolata; La Mura, Vincenzo; Capasso, Mario; Tiribelli, Claudio; Torella, Roberto; Iolascon, Achille

    2009-01-01

    The poor response to antiviral treatment of hepatitis C virus (HCV)-infected patients with genotype 1b has been associated with a higher prevalence of metabolic syndrome. However, the molecular link between these clinical entities is not clear. The goal of this study was to clarify the role of genotype 1b and 2 in the genetic expression of suppressor of cytokine signaling 3 (SOCS3) and insulin receptor substrate 1 (IRS-1). We infected human hepatocellular carcinoma cell line (HepG2) cells with human HCV genotype 1b or 2 and measured the gene and protein expression of SOCS3 at various times. We also evaluated impairment in the insulin pathway by analysis of IRS-1 and phospho-AKT. For the control, we used HepG2 cell cultures treated with non-infectious serum. We also demonstrated the occurrence of HCV infection by the detection of both positive and negative strands in the cells and culture medium. To test infection of the HepG2 cells, we performed quantitative real-time polymerase chain reaction (qRT-PCR) of viral load at different time points. We analyzed the viral genotype in the pellet and supernatant. At each time point, we found positive and negative strands in the infected cells, while in the medium we found positive, but no negative strands. We also detected the presence of the correct genotype in the medium. Two weeks following infection when the viral load was higher, we tested genotype 1b and 2 infected cells. SOCS3 gene expression was significantly higher in genotype 1b-infected cells (median 2.56; mean 2.82+/-0.59) compared with genotype 2 (median 1.34; mean 1.46+/-0.31) (p=0.04) and control cells (median 1.09; mean 1.02+/-0.11, p=0.02). There was no difference between cells exposed to genotype 2 and control cells. Conversely, IRS-1 was significantly lower in genotype 1b-infected cells (median 15.97; mean 15.45+/-0.67) compared with genotype 2-infected cells (median 16.45; mean 16.44+/-0.01, p=0.04). Statistically significant differences were seen when

  18. Genética y derechos humanos

    OpenAIRE

    Gómez-Ojero y Martínez, Luis

    2016-01-01

    [ES]La tesis se propone realizar un análisis de los temás más novedosos en ciencia genética y del estado de la cuestión relativa a los Derechos Humanos y la Genética partiendo de los textos más relevantes, tanto a nivel jurídico como meramente institucional que, como en el caso de Naciones Unidas, tanta importancia tienen en la influencia que de facto suele lograr sobre el Derecho Internacional, el Derecho de la Unión Europea y, por reflejo, indirectamente, en los Ordenamientos Jurídicos Inte...

  19. Zum gotischen gen. pl. auf-ë

    Directory of Open Access Journals (Sweden)

    M. Grošelj

    1964-12-01

    Full Text Available Bekanntlich ist die got. Endung -ē eine Besonderheit, der in den übrigen germ. Sprachen nichts Vergleichbares gegenübersteht. Man nimmt wohl -allgemein an, dass es sich um eine innergot. Neuschöpfung handelt; auch ist eine idg. Endung des Gen. Pl. -ēm; linbelegt. Folgender Erklärungsversuch geht von der Annahme aus, dass die Triebkraft für dfese Neuschöpfupg in der Neigung zur Differenzierung des Maskulinums vom Femininum auf -ō (gibō : dagē zu suchen ist. Diese Neigung ist besonders im Nom. und Gen. bemerkbar.

  20. Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway

    Directory of Open Access Journals (Sweden)

    Xiaona Zhao

    2016-05-01

    Full Text Available Trans-fatty acid consumption has been reported as a risk factor for metabolic disorders and targeted organ damages. Nonetheless, little is known about the roles and mechanisms of trans-fatty acids in obesity, insulin resistance (IR and hepatic steatosis. Adult C57BL/6 male mice were fed with four different diets for 20 weeks: normal diet (ND, high fat diet (HFD, low trans-fatty acids diet (LTD and high trans-fatty acid diet (HTD. The diet-induced metabolic disorders were assessed by evaluating body weight, glucose tolerance test, hepatic steatosis and plasma lipid profiles post 20-week diet. Histological (H&E, Oil-Red-O staining and western blot analysis were employed to assess liver steatosis and potential signaling pathways. After 20-weeks of diet, the body weights of the four groups were 29.61 ± 1.89 g (ND, 39.04 ± 4.27 g (HFD, 34.09 ± 2.62 g (LTD and 43.78 ± 4.27 g (HTD (p < 0.05, respectively. HFD intake significantly impaired glucose tolerance, which was impaired further in the mice consuming the HTD diet. The effect was further exacerbated by HTD diet. Moreover, the HTD group exhibited significantly more severe liver steatosis compared with HFD group possibly through regulating adipose triglyceride lipase. The group consuming the HTD also exhibited significantly reduced levels of IRS1, phosphor-PKC and phosphor-AKT. These results support our hypothesis that consumption of a diet high in trans-fatty acids induces higher rates of obesity, IR and hepatic steatosis in male C57BL/6 mice, possibly by suppressing the IRS1dependent pathway.

  1. Over-expression of NYGGF4 inhibits glucose transport in 3T3-L1 adipocytes via attenuated phosphorylation of IRS-1 and Akt

    Institute of Scientific and Technical Information of China (English)

    Chun-mei ZHANG; Xiao-hui CHEN; Bin WANG; Feng LIU; Xia CHI; Mei-ling TONG; Yu-hui NI; Rong-hua CHEN; Xi-rong GUO

    2009-01-01

    Aim: NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese patients. The purpose of this study was to investigate the effects of NYGGF4 on basal and insulin-stimulated glucose uptake in mature 3T3-L1 adipocytes and to understand the underlying mechanisms. Methods: 3T3-L1 preadipocytes transfected with either an empty expression vector (pcDNA3.1Myc/His B) or an NYGGF4 expression vector were differentiated into mature adipocytes. Glucose uptake was determined by measuring 2-deoxy-D-[3H]glucose uptake into the adipocytes. Immunoblotting was performed to detect the translocation of insulin-sensitive glu-cose transporter 4 (GLUT4). Immunoblotting also was used to measure the phosphorylation and total protein contents of insulin signaling proteins such as the insulin receptor (IR), insulin receptor substrate (IRS)-I, Akt, ERK1/2, p38, and JNK. Results: NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake and impaired insulin-stimulated GLUT4 translocation. It also diminished insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phos-phorylation of Akt without affecting the phosphorylation of IR, ERK1/2, p38, and JNK. Conclusion: NYGGF4 regulates the functions of IRS-1 and Akt, decreases GLUT4 translocation and reduces glucose uptake in response to insulin. These observations highlight the potential role of NYGGF4 in glucose homeostasis and possibly in the pathogenesis of obesity.

  2. Colon Cancer on The Rise Among Gen Xers, Millennials

    Science.gov (United States)

    ... Colon Cancer on the Rise Among Gen Xers, Millennials And an old adversary -- the obesity epidemic -- may ... their early 50s and younger -- Gen Xers and millennials -- are experiencing significant increases in colon and rectal ...

  3. Effect of dehydroepiandrosterone (DHEA) on Akt and protein kinase C zeta (PKCζ) phosphorylation in different tissues of C57BL6, insulin receptor substrate (IRS)1(-/-), and IRS2(-/-) male mice fed a high-fat diet.

    Science.gov (United States)

    Aoki, Kazutaka; Tajima, Kazuki; Taguri, Masataka; Terauchi, Yasuo

    2016-05-01

    We have previously reported that dehydroepiandrosterone (DHEA) suppresses the activity and mRNA expression of the hepatic gluconeogenic enzyme glucose-6-phosphatase (G6Pase), and hepatic glucose production in db/db mice. Tyrosine phosphorylation levels of Insulin receptor substrate (IRS)1 and IRS2 reportedly differ between the liver and muscle tissue and the effect of DHEA on insulin signaling has not been elucidated. Therefore, we examined DHEA's effect on the liver and muscle tissue of IRS1(-/-) and IRS2(-/-) mice. Eight-week-old male C57BL6, IRS1(-/-), and IRS2(-/-) mice were fed a high-fat diet (HFD), or an HFD containing 0.2% DHEA for 4 weeks. In a separate experiment, 8-week-old male C57BL6 mice were fed an HFD or an HFD containing 0.2% androstenedione for 4 weeks. In an insulin tolerance test, DHEA administration decreased the initial plasma glucose levels in the C57BL6, IRS1(-/-), and IRS2(-/-) mice but did not decrease the ratios to the basal blood glucose level. Although DHEA administration increased Akt phosphorylation in the liver of the C57BL6, IRS1(-/-), and IRS2(-/-) mice, androstenedione administration did not increase Akt phosphorylation in the liver of C57BL6 mice. DHEA administration did not increase Akt and PKCζ phosphorylation in the muscle tissue of C57BL6, IRS1(-/-), or IRS2(-/-) mice. However, androstenedione administration increased Akt and PKCζ phosphorylation in the muscle tissue of C57BL6 mice. These findings suggest that the effect of DHEA on insulin action in the liver is self-mediated by DHEA or DHEA sulfate (DHEA-S) in the presence of IRS1, IRS2, or both. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. 77 FR 2342 - Fifth Meeting: RTCA, Next Gen Advisory Committee

    Science.gov (United States)

    2012-01-17

    ... TRANSPORTATION Federal Aviation Administration Fifth Meeting: RTCA, Next Gen Advisory Committee AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA, NextGen... RTCA, NextGen Advisory Committee. DATES: The meeting will be held February 3, 2012, from 9:30...

  5. A genética das epilepsias

    National Research Council Canada - National Science Library

    Lopes-Cendes, Iscia

    2008-01-01

    .... Mais recentemente, estudos de genética molecular e estratégias de descoberta de genes foram usados para revelar os mecanismos moleculares e celulares envolvidas em diversas síndromes epilépticas mendelianas. SÍNTESE DOS DADOS...

  6. 25 Years of GenBank

    Science.gov (United States)

    ... to science over the last 25 years, the National Center for Biotechnology Information (NCBI), National Library of Medicine (NLM), and NIH held ... GenBank has been a critical research tool," says NCBI Director David Lipman, "enabling much of the progress ...

  7. Preserving Accuracy in GenBank

    DEFF Research Database (Denmark)

    Bidartondo, M.I.; Bruns, T. D.; Blackwell, M.

    2008-01-01

    GenBank, the public repository for nucleotide and protein sequences, is a critical resource for molecular biology, evolutionary biology, and ecology. While some attention has been drawn to sequence errors (1), common annotation errors also reduce the value of this database. In fact, for organisms...

  8. Effects of Cordyceps Polysaccharide on InsR/IRS-1 Pathway and Glucose Metabolism in Type 2 Diabetic Mice%虫草多糖对2型糖尿病小鼠InsR/IRS-1通路及糖代谢的影响

    Institute of Scientific and Technical Information of China (English)

    刘雪芹; 于湄; 张燕; 魏丽莉

    2011-01-01

    Objective: To investigate the effects of Cordyceps polysaccharide on InsR/IRS-1 pathway and glucose metabolism in type 2 diabetic mice. Method: Normal male Balb/c mice were ip injected with low-dose of streptozotocin (STZ) and fed with high fat diet for induction of type 2 diabetes related insulin resistance (T2DM-IR). Cordyceps polysaccharide (200 and 400 mg·kg- 1 ) was administered orally to Fat/Fed/STZ induced diabetic mice for 28 d. Western blotting was applied to detect the expression levels of insulin receptor (InsR) ,insulin receptor substrate-1 ( IRS-1 ) and glucose transporter typo 4 (GLUT4) in skeletal muscle of mice. Moreover,the hepatic glucokinase (GK) and phosphofructokinase (PFK) activities were measured to investigate the effect of Cordyceps polysaccharide on glucose metabolism. Result: Treatment with Cordyceps polysaccharide could markedly improve the deviant expression of InsR, IRS-1 and GLUT4 molecules in the diabetic mice (P < 0. 05 ). Furthermore, Cordyceps polysaccharide significantly increased the GK and PFK activity in liver in a dose dependent manner when compared with the diabetic control (P <0. 05 and P <0. 01 ,respectively). Conclusion: It is demonstrated that Cordyceps polysaccharide can enhance insulin signaling transduction and glucose metabolism resulting in reducing insulin resistance and hyperglycemia symptom eventually. These beneficial effects of Cordyceps polysaccharide could be helpful for the treatment of type 2 diabetes related insulin resistance in clinics.%目的:观察虫草多糖对2型糖尿病小鼠InsR/IRS-1通路及糖代谢的影响.方法:采用高脂饲料联合腹腔注射小剂量链脲佐菌素(STZ,25 mg·kg-1)建立胰岛素抵抗2型糖尿病模型,每日给予不同剂量虫草多糖(200,400 nag·kg-1)治疗,28 d后,免疫印迹法检测实验小鼠骨骼肌内胰岛素受体(InsR)、胰岛素受体底物(IRS-1)及葡萄糖转运蛋白(GLUT4)的表达水平.检测肝脏葡萄糖激酶(GK)、磷酸果

  9. Shaping a high-mass star-forming cluster through stellar feedback. The case of the NGC 7538 IRS 1-3 complex

    Science.gov (United States)

    Frau, P.; Girart, J. M.; Zhang, Q.; Rao, R.

    2014-07-01

    Context. NGC 7538 IRS 1-3 is a high-mass star-forming cluster with several detected dust cores, infrared sources, (ultra)compact H II regions, molecular outflows, and masers. In such a complex environment, interactions and feedback among the embedded objects are expected to play a major role in the evolution of the region. Aims: We study the dust, kinematic, and polarimetric properties of the NGC 7538 IRS 1-3 region to investigate the role of the different forces in the formation and evolution of high-mass star-forming clusters. Methods: We performed SMA high angular resolution observations at 880 μm with the compact configuration. We developed the RATPACKS code to generate synthetic velocity cubes from models of choice to be compared to the observational data. To quantify the stability against gravitational collapse we developed the "mass balance" analysis that accounts for all the energetics on core scales. Results: We detect 14 dust cores from 3.5 M⊙ to 37 M⊙ arranged in two larger scale structures: a central bar and a filamentary spiral arm. The spiral arm presents large-scale velocity gradients in H13CO+ 4-3 and C17O 3-2, and magnetic field segments aligned well to the dust main axis. The velocity gradient is reproduced well by a spiral arm expanding at 9 km s-1 with respect to the central core MM1, which is known to power a large precessing outflow. The energy of the outflow is comparable to the spiral-arm kinetic energy, which dominates gravitational and magnetic energies. In addition, the dynamical ages of the outflow and spiral arm are comparable. On core scales, those embedded in the central bar seem to be unstable against gravitational collapse and prone to forming high-mass stars, while those in the spiral arm have lower masses that seem to be supported by non-thermal motions and magnetic fields. Conclusions: The NGC 7538 IRS 1-3 cluster seems to be dominated by protostellar feedback. The dusty spiral arm appears to be formed in a snowplow fashion

  10. Gen IV Materials Handbook Implementation Plan

    Energy Technology Data Exchange (ETDEWEB)

    Rittenhouse, P.; Ren, W.

    2005-03-29

    A Gen IV Materials Handbook is being developed to provide an authoritative single source of highly qualified structural materials information and materials properties data for use in design and analyses of all Generation IV Reactor Systems. The Handbook will be responsive to the needs expressed by all of the principal government, national laboratory, and private company stakeholders of Gen IV Reactor Systems. The Gen IV Materials Handbook Implementation Plan provided here addresses the purpose, rationale, attributes, and benefits of the Handbook and will detail its content, format, quality assurance, applicability, and access. Structural materials, both metallic and ceramic, for all Gen IV reactor types currently supported by the Department of Energy (DOE) will be included in the Gen IV Materials Handbook. However, initial emphasis will be on materials for the Very High Temperature Reactor (VHTR). Descriptive information (e.g., chemical composition and applicable technical specifications and codes) will be provided for each material along with an extensive presentation of mechanical and physical property data including consideration of temperature, irradiation, environment, etc. effects on properties. Access to the Gen IV Materials Handbook will be internet-based with appropriate levels of control. Information and data in the Handbook will be configured to allow search by material classes, specific materials, specific information or property class, specific property, data parameters, and individual data points identified with materials parameters, test conditions, and data source. Details on all of these as well as proposed applicability and consideration of data quality classes are provided in the Implementation Plan. Website development for the Handbook is divided into six phases including (1) detailed product analysis and specification, (2) simulation and design, (3) implementation and testing, (4) product release, (5) project/product evaluation, and (6) product

  11. Associations of Haplotypes Upstream of IRS1 with Insulin Resistance, Type 2 Diabetes, Dyslipidemia, Preclinical Atherosclerosis, and Skeletal Muscle LOC646736 mRNA Levels

    Directory of Open Access Journals (Sweden)

    Selma M. Soyal

    2015-01-01

    Full Text Available The genomic region ~500 kb upstream of IRS1 has been implicated in insulin resistance, type 2 diabetes, adverse lipid profile, and cardiovascular risk. To gain further insight into this chromosomal region, we typed four SNPs in a cross-sectional cohort and subjects with type 2 diabetes recruited from the same geographic region. From 16 possible haplotypes, 6 haplotypes with frequencies >0.01 were observed. We identified one haplotype that was protective against insulin resistance (determined by HOMA-IR and fasting plasma insulin levels, type 2 diabetes, an adverse lipid profile, increased C-reactive protein, and asymptomatic atherosclerotic disease (assessed by intima media thickness of the common carotid arteries. BMI and total adipose tissue mass as well as visceral and subcutaneous adipose tissue mass did not differ between the reference and protective haplotypes. In 92 subjects, we observed an association of the protective haplotype with higher skeletal muscle mRNA levels of LOC646736, which is located in the same haplotype block as the informative SNPs and is mainly expressed in skeletal muscle, but only at very low levels in liver or adipose tissues. These data suggest a role for LOC646736 in human insulin resistance and warrant further studies on the functional effects of this locus.

  12. First-principles study of Sr2Ir1-xRhxO4: charge transfer, spin-orbit coupling change, and the metal-insulator transition

    Science.gov (United States)

    Sim, Jae-Hoon; Kim, Heung-Sik; Han, Myung Joon

    2015-03-01

    Using first-principles density functional theory (DFT) calculations, we investigated the electronic structure of Rh-doped iridate, Sr2Ir1-xRhxO4 for which the doping (x) dependent metal-insulator transition (MIT) has been reported experimentally and the controversial discussion developed regarding the origin of this transition. Our DFT+U calculation shows that the value of remains largely intact over the entire doping range considered here (x = 0 . 0 , 0 . 125 , 0 . 25 , 0 . 50 , 0 . 75 , and 1 . 0) in good agreement with the branching ratio measured by x-ray absorption spectroscopy. Also contrary to a previous picture to explain MIT based on the charge transfer between the transition-metal sites, our calculation clearly shows that those sites remain basically isoelectronic while the impurity bands of predominantly rhodium character are introduced near the Fermi level. As the doping increases, this impurity band overlaps with lower Hubbard band of iridium, leading to metal-insulator transition. The results will be discussed with comparison to the case of Ru doping. Computational resources were suported by The National Institute of Supercomputing and Networking/Korea Institute of Science and Technology Information with supercomputing resources including technical spport (Grant No. KSC-2013-C2-23).

  13. A spider-like outflow in Barnard 5 - IRS 1: The transition from a collimated jet to a wide-angle outflow?

    CERN Document Server

    Zapata, Luis A; Brassfield, Erin; Palau, Aina; Patel, Nimesh; Pineda, Jaime E

    2014-01-01

    We present line and continuum observations made with the Submillimeter Array (SMA) of the young stellar object Barnard 5 - IRS1 located in the Perseus molecular cloud. Our $^{12}$CO(2-1) line observations resolve the high-velocity bipolar northeast-southwest outflow associated with this source. We find that the outflowing gas shows different structures at three different velocity regimes, in both lobes, resulting in a spider-like morphology. In addition to the low-velocity, cone-like (wide-angle) lobes that have previously been observed, we report the presence of intermediate-velocity parabolic shells emerging very close to the Class I protostar, as well as high velocity molecular bullets that appear to be associated to the optical/IR jet emanating from this source. These compact high-velocity features reach radial velocities of about 50 km s$^{-1}$ away from the cloud velocity. We interpret the peculiar spider-like morphology is a result of the molecular material being entrained by a wind with both a collima...

  14. Hosting of surface states in spin-orbit induced projected bulk band gaps of W(1 1 0) and Ir(1 1 1)

    Science.gov (United States)

    Elmers, H. J.; Kutnyakhov, D.; Chernov, S. V.; Medjanik, K.; Fedchenko, O.; Zaporozhchenko-Zymakova, A.; Ellguth, M.; Tusche, C.; Viefhaus, J.; Schönhense, G.

    2017-06-01

    Spin-momentum locking of surface states has attracted great interest in recent years due to envisioned technological applications in the field of spintronics. Normal metal surfaces like W(1 1 0) and Ir(1 1 1) show surface states with energy dispersions and spin-polarization textures, which are reminiscent of topologically non-trivial surface states. In order to understand this phenomenon the connection of bulk and surface states has to be explored. Using time-of-flight momentum microscopy with soft x-ray excitation, we present a comprehensive analysis of the bulk bands of W and Ir. Surface states are determined by the same method with photon excitation in the vacuum ultraviolet region. The superposition of both spectral densities reveals the hosting of surface states within the gap structure of bulk bands projected on the surface Brillouin zone. Quantitative differences in the extension of experimental and theoretical local band gaps indicate an underestimation of electron correlation effects in theory.

  15. Safety Assurance in NextGen

    Science.gov (United States)

    HarrisonFleming, Cody; Spencer, Melissa; Leveson, Nancy; Wilkinson, Chris

    2012-01-01

    The generation of minimum operational, safety, performance, and interoperability requirements is an important aspect of safely integrating new NextGen components into the Communication Navigation Surveillance and Air Traffic Management (CNS/ATM) system. These requirements are used as part of the implementation and approval processes. In addition, they provide guidance to determine the levels of design assurance and performance that are needed for each element of the new NextGen procedures, including aircraft, operator, and Air Navigation and Service Provider. Using the enhanced Airborne Traffic Situational Awareness for InTrail Procedure (ATSA-ITP) as an example, this report describes some limitations of the current process used for generating safety requirements and levels of required design assurance. An alternative process is described, as well as the argument for why the alternative can generate more comprehensive requirements and greater safety assurance than the current approach.

  16. Esquizofrenia, genética y complejidad

    OpenAIRE

    Jensen-Pennington, Henning

    2011-01-01

    El artículo plantea la necesidad de no sobresimplificar fenómenos complejos como el de la esquizofrenia, pues su etiología implica la interacción de aspectos genéticos, biológicos y sociopsicológicos. Se plantea que el mismo conocimiento genético imposibilita la asunción de estas posiciones radicales, razón por la cual la investigación biológica actúa con cautela en la formulación de hipótesis causales. No obstante, se pone de manifiesto la interpretación inapropiada de los resultados de inve...

  17. Standby for the Gen-Set

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1998-08-01

    Diesel Gen-Sets are hardly romantic instruments, yet they do save lives. Humming away in the corner for back-up power or emergency generation, this compact form of power supply, keeps hospital lights on, computers going and often proves a vital, if unnoticed addition to festivals and outdoor events. And as many master engine builders have discovered, this is a rapidly growing market. (author)

  18. Genética do autismo

    OpenAIRE

    Carvalheira,Gianna; VERGANI, Naja; Brunoni,Décio

    2004-01-01

    O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.

  19. Genética de comunidades

    Directory of Open Access Journals (Sweden)

    M. Verdú

    2009-01-01

    Full Text Available Los estudios sobre los procesos responsables del ensamblaje de las especies en comunidades ecológicas tradicionalmente han considerado a las especies como unidades de estudio. Sin embargo, dentro de las especies existe gran cantidad de variación genotípica y fenotípica que puede finalmente ser decisiva en el resultado de las interacciones con el resto de especies en la comunidad (competencia, mutualismo, etc. Recientemente se ha propuesto una nueva regla de ensamblaje de las comunidades que sugiere que la composición genética de una población afecta a la estructura y composición de la comunidad (es decir alfenotipo de la comunidad. Apoyando esta regla, varios estudios han demostrado que los cambios en la diversidad genética de poblaciones de plantas conllevan cambios predecibles en sus comunidades asociadas de herbívoros y predadores. Más allá de la visión poblacional, la nueva genética de comunidades pretende demostrar 1 que el genotipo particular de ciertos individuos es responsable del fenotipo de la comunidad, 2 que el fenotipo de la comunidad es heredable, y por lo tanto 3 que las comunidades evolucionan. Tras explicar estos tres conceptos, se discute el valor que esta aproximación puede tener desde el punto de vista de la biología de la conservación.

  20. Generic classification of the Archiborborinae (Diptera: Sphaeroceridae), with a revision of Antrops Enderlein, Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov.

    Science.gov (United States)

    Kits, Joel H; Marshall, Stephen A

    2013-01-01

    The Archiborborinae comprise a diverse clade of flies in the family Sphaeroceridae. We here revise the generic classification, redefining the genus Antrops Enderlein and naming 5 new genera: Boreantrops gen. nov., Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov. The genus Archiborborus, until recently a paraphyletic assemblage including most of the described species in the subfamily, is treated as a junior synonym of Antrops (syn. nov.) We revise the genera Antrops (53 species, including 40 sp. nov.: Antrops anovariegatus, Antrops aurantifemur, Antrops baeza, Antrops bellavista, Antrops biflavus, Antrops bucki, Antrops carpishensis, Antrops cochabamba, Antrops cochinoca, Antrops coniobaptos, Antrops coroico, Antrops cotopaxi, Antrops didactylos, Antrops diversipennis, Antrops eurus, Antrops fulgiceps, Antrops fuliginosus, Antrops guandera, Antrops guaramacalensis, Antrops inca, Antrops juninensis, Antrops mucarensis, Antrops niger, Antrops papallacta, Antrops pecki, Antrops podocarpus, Antrops quadrilobus, Antrops siberia, Antrops sierrazulensis, Antrops tachira, Antrops tequendama, Antrops tetrastichus, Antrops tumbrensis, Antrops unduavi, Antrops variegatus, Antrops versabilis, Antrops vittatus, Antrops yungas, and Antrops zongo and the following comb. nov.: Antrops annulatus (Richards), Antrops chaetosus (Richards), Antrops femoralis (Blanchard), Antrops hirtus (Bigot), Antrops maculipennis (Duda), Antrops maximus (Richards), Antrops microphthalmus (Richards), Antrops quadrinotus (Bigot), Antrops setosus (Duda), Antrops simplicimanus (Richards), Antrops nitidicollis (Becker), and Antrops orbitalis (Duda)), Coloantrops (1 species: Coloantrops daedalus, sp. nov.), Maculantrops (2 species, Maculantrops hirtipes (Macquart) comb. nov. and Maculantrops altiplanus, sp. nov.), Photoantrops (1 species: Pho-toantrops echinus sp. nov.), and Poecilantrops (10 species: Poecilantrops baorucensis, Poecilantrops boraceiensis

  1. Alkaline pH induces IRR-mediated phosphorylation of IRS-1 and actin cytoskeleton remodeling in a pancreatic beta cell line.

    Science.gov (United States)

    Deyev, Igor E; Popova, Nadezhda V; Serova, Oxana V; Zhenilo, Svetlana V; Regoli, Marì; Bertelli, Eugenio; Petrenko, Alexander G

    2017-07-01

    Secretion of mildly alkaline (pH 8.0-8.5) juice to intestines is one of the key functions of the pancreas. Recent reports indicate that the pancreatic duct system containing the alkaline juice may adjoin the endocrine cells of pancreatic islets. We have previously identified the insulin receptor-related receptor (IRR) that is expressed in islets as a sensor of mildly alkaline extracellular media. In this study, we show that those islet cells that are in contact with the excretory ducts are also IRR-expressing cells. We further analyzed the effects of alkaline media on pancreatic beta cell line MIN6. Activation of endogenous IRR but not of the insulin receptor was detected that could be inhibited with linsitinib. The IRR autophosphorylation correlated with pH-dependent linsitinib-sensitive activation of insulin receptor substrate 1 (IRS-1), the primary adaptor in the insulin signaling pathway. However, in contrast with insulin stimulation, no protein kinase B (Akt/PKB) phosphorylation was detected as a result of alkali treatment. We observed overexpression of several early response genes (EGR2, IER2, FOSB, EGR1 and NPAS4) upon alkali treatment of MIN6 cells but those were IRR-independent. The alkaline medium but not insulin also triggered actin cytoskeleton remodeling that was blocked by pre-incubation with linsitinib. We propose that the activation of IRR by alkali might be part of a local loop of signaling between the exocrine and endocrine parts of the pancreas where alkalinization of the juice facilitate insulin release that increases the volume of secreted juice to control its pH and bicabonate content. Copyright © 2017 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  2. Climatic potential productivity and an IRS-1CWiFS image in Natural Park Los Alcornocales. Relationship with the standing forest biomass; Productividad Potencial Climatica y una imagen IRS-1CWiFS en el Parque Natural Los Alcornocales. Relacion con la biomasa forestal real

    Energy Technology Data Exchange (ETDEWEB)

    Cuevas, J. M.; Gonzalez-Alonso, F.; Roldan, A.; Huesca, M.

    2009-07-01

    It is studied the use of the Map of Forest Potential Productivity of Spain as information in base to which classify an Indian IRS-1C WiFS satellite image in Los Alcornocales Natural Park (Andalucia, Spain), a large forest area covered by natural forests of Mediterranean broad-leaved species, mainly cork oak (Quercus suber L.). Grouping the classes of climatic potential productivity of this map were obtained three macro classes that resulted significantly different among them at the 99% confidence level for the visible and the Normalized Difference Vegetation Index (NDVI) of the WiFS image. It was built a classifier using these macro classes of climatic potential productivity as ground truth areas. By maximum likelihood supervised classification of the NDVI were obtained classes who resulted significantly different among them at the 90% confidence level for the basal area from the Second Spanish National Forest Inventory ground plots located in the park. (Author) 60 refs.

  3. Das seneszenzassoziierte Gen HvS40 der Gerste

    OpenAIRE

    Trösch, Mirl

    2016-01-01

    In der vorliegenden Arbeit wurde das seneszenzassoziierte Gen HvS40 der Gerste als dual kodierendes Gen charakterisiert. Damit wurde ein solches Gen erstmals in Pflanzen beschrieben. Der alternative S40+1-Leserahmen, der den kanonischen Leserahmen im 5'-Bereich überragt, konnte auch in anderen monokotylen, jedoch nicht in dikotylen Arten gefunden werden. Das S40-Protein, das durch den kanonischen Leserahmen S40+3 kodiert wird, kann der pflanzenspezifischen Proteinfamilie DUF584 zugeordnet...

  4. Aconselhamento genético Genetic counseling

    Directory of Open Access Journals (Sweden)

    João Monteiro de Pina-Neto

    2008-08-01

    Full Text Available OBJETIVO: Esta revisão sobre aconselhamento genético (AG teve o objetivo de mostrar os conceitos atuais e os princípios filosóficos e éticos aceitos na grande maioria dos países e recomendados pela Organização Mundial da Saúde, as fases do processo, seus resultados e o impacto psicológico de uma doença genética em uma família. FONTES DOS DADOS: Os conceitos apresentados são baseados em uma síntese histórica da literatura sobre AG desde a década de 1930 até o momento atual, sendo que os artigos citados representam os principais trabalhos publicados e que hoje fundamentam a teoria e a prática do AG. SÍNTESE DOS DADOS: O AG modernamente é definido como um processo de comunicação que trata dos problemas humanos relacionados à ocorrência de uma doença genética em uma família. É fundamental que os profissionais da saúde conheçam os aspectos psicológicos desencadeados pela doença genética e como estes aspectos podem ser manejados. Vivemos ainda na genética humana e médica uma fase de predomínio dos aspectos técnicos e científicos e de pouca ênfase no estudo das reações emocionais e dos processos de adaptação das pessoas a estas doenças, o que leva ao baixo entendimento dos clientes sobre os fatos ocorridos, com conseqüências negativas sobre a vida familiar e para a sociedade. CONCLUSÕES: Conclui-se pela necessidade de que as famílias com doenças genéticas sejam encaminhadas para AG e que os profissionais desta área invistam mais na humanização do atendimento, desenvolvendo mais as técnicas do AG psicológico não-diretivo.OBJECTIVE: The objective of this review of genetic counseling (GC is to describe the current concepts and philosophical and ethical principles accepted by the great majority of countries and recommended by the World Health Organization, the stages of the process, its results and the psychological impact that a genetic disease has on a family. SOURCES: The concepts presented are

  5. TidGen Power System Commercialization Project

    Energy Technology Data Exchange (ETDEWEB)

    Sauer, Christopher R. [President & CEO; McEntee, Jarlath [VP Engineering & CTO

    2013-12-30

    ORPC Maine, LLC, a wholly-owned subsidiary of Ocean Renewable Power Company, LLC (collectively ORPC), submits this Final Technical Report for the TidGen® Power System Commercialization Project (Project), partially funded by the U.S. Department of Energy (DE-EE0003647). The Project was built and operated in compliance with the Federal Energy Regulatory Commission (FERC) pilot project license (P-12711) and other permits and approvals needed for the Project. This report documents the methodologies, activities and results of the various phases of the Project, including design, engineering, procurement, assembly, installation, operation, licensing, environmental monitoring, retrieval, maintenance and repair. The Project represents a significant achievement for the renewable energy portfolio of the U.S. in general, and for the U.S. marine hydrokinetic (MHK) industry in particular. The stated Project goal was to advance, demonstrate and accelerate deployment and commercialization of ORPC’s tidal-current based hydrokinetic power generation system, including the energy extraction and conversion technology, associated power electronics, and interconnection equipment capable of reliably delivering electricity to the domestic power grid. ORPC achieved this goal by designing, building and operating the TidGen® Power System in 2012 and becoming the first federally licensed hydrokinetic tidal energy project to deliver electricity to a power grid under a power purchase agreement in North America. Located in Cobscook Bay between Eastport and Lubec, Maine, the TidGen® Power System was connected to the Bangor Hydro Electric utility grid at an on-shore station in North Lubec on September 13, 2012. ORPC obtained a FERC pilot project license for the Project on February 12, 2012 and the first Maine Department of Environmental Protection General Permit issued for a tidal energy project on January 31, 2012. In addition, ORPC entered into a 20-year agreement with Bangor Hydro Electric

  6. Genética molecular del alcoholismo

    OpenAIRE

    Mauricio Rey-Buitrago

    2015-01-01

    El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a to...

  7. Gen IV Materials Handbook Functionalities and Operation

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Weiju [ORNL

    2009-12-01

    This document is prepared for navigation and operation of the Gen IV Materials Handbook, with architecture description and new user access initiation instructions. Development rationale and history of the Handbook is summarized. The major development aspects, architecture, and design principles of the Handbook are briefly introduced to provide an overview of its past evolution and future prospects. Detailed instructions are given with examples for navigating the constructed Handbook components and using the main functionalities. Procedures are provided in a step-by-step fashion for Data Upload Managers to upload reports and data files, as well as for new users to initiate Handbook access.

  8. Autismo: genética Autism: genetics

    Directory of Open Access Journals (Sweden)

    Abha R Gupta

    2006-05-01

    Full Text Available O autismo é um transtorno fortemente genético, com uma herdabilidade estimada de mais de 90%. Uma combinação de heterogeneidade fenotípica e o provável envolvimento de múltiplos loci que interagem entre si dificultam os esforços de descobertas de genes. Conseqüentemente, a etiologia genética dos transtornos relacionados ao autismo permanece, em grande parte, desconhecida. Nos últimos anos, a convergência entre tecnologias genômicas em rápido avanço, a finalização do projeto genoma humano e os crescentes e exitosos esforços em colaboração para aumentar o número de pacientes disponíveis para estudo conduziram às primeiras pistas sólidas sobre as origens biológicas desses transtornos. Este artigo revisará a literatura até nossos dias, resumindo os resultados de estudos de ligação genética, citogenéticos e de genes candidatos com um foco no progresso recente. Além disso, são consideradas as vias promissoras para pesquisas futuras.Autism is a strongly genetic disorder, with an estimated heritability of greater than 90%. A combination of phenotypic heterogeneity and the likely involvement of multiple interacting loci have hampered efforts at gene discovery. As a consequence, the genetic etiology of the spectrum of autism related disorders remains largely unknown. Over the past several years, the convergence of rapidly advancing genomic technologies, the completion of the human genome project, and increasingly successful collaborative efforts to increase the number of patients available for study have led to the first solid clues to the biological origins of these disorders. This paper will review the literature to date summarizing the results of linkage, cytogenetic, and candidate gene studies with a focus on recent progress. In addition, promising avenues for future research are considered.

  9. Genética molecular del alcoholismo

    Directory of Open Access Journals (Sweden)

    Mauricio Rey-Buitrago

    2015-07-01

    Full Text Available El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a todo el genoma, de expresión y epigenéticos han identificado una amplia gama de variantes genéticas y epigenéticas, blancos para los estudios de susceptibilidad, diagnóstico y tratamiento farmacológico. Actualmente se comprenden mucho más estas relaciones y el desarrollo rápido de nuevas metodologías de estudio promete continuar este proceso, así como la generación de algoritmos de diagnóstico, prevención y tratamientos más acertados y confiables.

  10. Towards an International Culture: Gen Y Students and SNS?

    Science.gov (United States)

    Lichy, Jessica

    2012-01-01

    This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

  11. Gen-duplikationer - evolutionens eksperimentarium med et eksempel fra hvede

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh; Dionisio, Giuseppe; Brinch-Pedersen, Henrik

    2013-01-01

    Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag.......Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag....

  12. Towards an International Culture: Gen Y Students and SNS?

    Science.gov (United States)

    Lichy, Jessica

    2012-01-01

    This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

  13. Gen-duplikationer - evolutionens eksperimentarium med et eksempel fra hvede

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh; Dionisio, Giuseppe; Brinch-Pedersen, Henrik

    2013-01-01

    Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag.......Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag....

  14. Biometris GenStat Procedure Library Manual 12th Edition

    NARCIS (Netherlands)

    Goedhart, P.W.; Thissen, J.T.N.M.

    2009-01-01

    The Biometris GenStat Procedure Library contains procedures in which new and existing statistical methodology is implemented, as well as procedures to make GenStat more user-friendly. The Biometris library can be used and distributed freely. The Library is standardly distributed over the Dutch

  15. Biometris GenStat Procedure Library Manual 13th Edition

    NARCIS (Netherlands)

    Goedhart, P.W.; Thissen, J.T.N.M.

    2010-01-01

    The Biometris GenStat Procedure Library contains procedures in which new and existing statistical methodology is implemented, as well as procedures to make GenStat more user-friendly. The Biometris library can be used and distributed freely. The Library is standardly distributed over the Dutch

  16. 78 FR 8108 - NextGen Solutions Vendors Guide

    Science.gov (United States)

    2013-02-05

    ... Organization's (ICAO) Aviation System Block Upgrade (ASBU) initiative. The NextGen solutions address the ICAO... help implement ICAO-consistent NextGen solutions. The guide will highlight the U.S. producers and... to aviation system upgrades) Example: Engineering Services More information on the four ICAO...

  17. GenCade Version 1 Quick-Start Guide: How to Start a Successful GenCade Project

    Science.gov (United States)

    2015-03-01

    Properly defining the inlets is a crucial part of a GenCade project and can be difficult. A user should become familiar with the Inlet Reservoir Model ( IRM ...GenCade Report 2 provide additional documentation on IRM variable names and functions. 3.9.4 Export data The data may easily be exported to a text

  18. Gen-Umwelt-Interaktionen und Gen-Umwelt-Korrelationen bei psychiatrischen Erkrankungen

    Directory of Open Access Journals (Sweden)

    Winkler D

    2010-01-01

    Full Text Available Die seit Langem bestehende Frage, in welchem Ausmaß Anlage und Umwelt zu psychologischen Merkmalen und psychiatrischen Erkrankungen beitragen, wird durch Ergebnisse von Zwillingsstudien und in letzter Zeit durch Untersuchungen des Zusammenhangs zwischen molekulargenetischen Merkmalen und Umwelteinflüssen bereichert. Eine Gen-Umwelt-Interaktion liegt dann vor, wenn genetische Faktoren die Auswirkungen von Umweltbedingungen modulieren. Die Genetik kann weiters die Wahrscheinlichkeit der Exposition gegenüber bestimmten Umwelteinflüssen verändern, was als Gen- Umwelt-Korrelation bezeichnet wird. Beide Phänomene liegen aber häufig gleichzeitig vor, was eine besondere Herausforderung für die Konzeption von wissenschaftlichen Studien darstellt.

  19. Delineamento de experimentos em genética genômica Experimental design in genetical genomics

    OpenAIRE

    Guilherme Jordão de Magalhães Rosa

    2007-01-01

    Genética genômica é um termo utilizado para representar o estudo de processos genéticos controladores de caracteres fenotípicos de herança complexa, a partir da análise conjunta de informação relativa a fenótipos, estruturas de parentesco, marcadores moleculares e expressão gênica. Estudos de genética genômica são utilizados, por exemplo, para a estimação da herdabilidade de níveis de transcrição, para o mapeamento de locos controladores da expressao gênica (eQTL, do inglês expression Quantit...

  20. Transferencia genética en Plantas

    OpenAIRE

    Carbonero Zalduegui, Pilar; García Olmedo, Francisco

    1985-01-01

    La mejora genética de las especies vegetales cultivadas ha sido, y continuará siendo, una de las armas más decisivas en la lucha permanente por mantener los incrementos de la producción de alimentos por delante del crecimiento de la población humana. Puede afirmarse que el hombre ha practicado de un modo empírico la mejora vegetal desde que, en los albores de la revolución agrícola, hace alrededor de 10.000 años, inició su gradual conversión de cazador-recolector en agricultor. Sin embargo, e...

  1. NextGen Future Safety Assessment Game

    Science.gov (United States)

    Ancel, Ersin; Gheorghe, Adrian; Jones, Sharon Monica

    2011-01-01

    The successful implementation of the next generation infrastructure systems requires solid understanding of their technical, social, political and economic aspects along with their interactions. The lack of historical data that relate to the long-term planning of complex systems introduces unique challenges for decision makers and involved stakeholders which in turn result in unsustainable systems. Also, the need to understand the infrastructure at the societal level and capture the interaction between multiple stakeholders becomes important. This paper proposes a methodology in order to develop a holistic approach aiming to provide an alternative subject-matter expert (SME) elicitation and data collection method for future sociotechnical systems. The methodology is adapted to Next Generation Air Transportation System (NextGen) decision making environment in order to demonstrate the benefits of this holistic approach.

  2. Innatismo y control genético

    Directory of Open Access Journals (Sweden)

    Sergio Daniel Barberis

    2013-12-01

    Full Text Available Mi objetivo en este artículo es defender una elucidación reduccionista parcial del concepto de lo innato en ciencias cognitivas. En primer lugar, especifico las condiciones mínimas para una elucidación de lo innato. En segundo lugar, presento los principales enfoques que se han adoptado en la discusión, es decir, las propuestas reduccionistas, autonomistas y escépticas. Luego de ofrecer argumentos versus el escepticismo y las propuestas autonomistas, presento en detalle lo que considero es una elucidación parcial de lo innato en términos de control genético, mostrando cómo un concepto de la biología del desarrollo puede ser provechosamente utilizado para elucidar el concepto de lo innato en ciencias cognitivas. Por último, explicito las ventajas filosóficas de mi propuesta.

  3. α-Lipoic acid protects 3T3-L1 adipocytes from NYGGF4 (PID1) overexpression-induced insulin resistance through increasing phosphorylation of IRS-1 and Akt.

    Science.gov (United States)

    Wang, Yu-mei; Lin, Xiao-fei; Shi, Chun-mei; Lu, Lan; Qin, Zhen-Ying; Zhu, Guan-zhong; Cao, Xin-guo; Ji, Chen-bo; Qiu, Jie; Guo, Xi-rong

    2012-06-01

    NYGGF4 (also called PID1) was demonstrated that it may be related to the development of obesity-related IR. We aimed in the present study to further elucidate the effects of NYGGF4 on IR and the underlying mechanisms through using α-Lipoic acid (LA) treatment, which could facilitate glucose transport and utilization in fully differentiated adipocytes. Our data showed that the LA pretreatment strikingly enhanced insulin-stimulated glucose uptake through increasing GLUT4 translocation to the PM in NYGGF4 overexpression adipocytes. The reactive oxygen species (ROS) levels in NYGGF4 overexpression adipocytes were strikingly enhanced, which could be decreased by the LA pretreatment. NYGGF4 overexpression resulted in significant inhibition of tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt, whereas incubation with LA strongly activated IRS-1 and Akt phosphorylation in NYGGF4 overexpression adipocytes. These results suggest that LA protects 3T3-L1 adipocytes from NYGGF4-induced IR partially through increasing phosphorylation of IRS-1 and Akt and provide evidence that NYGGF4 may be a potential target for the treatment of obesity and obesity-related IR.

  4. RxGen General Optical Model Prescription Generator

    Science.gov (United States)

    Sigrist, Norbert

    2012-01-01

    RxGen is a prescription generator for JPL's in-house optical modeling software package called MACOS (Modeling and Analysis for Controlled Optical Systems), which is an expert optical analysis software package focusing on modeling optics on dynamic structures, deformable optics, and controlled optics. The objectives of RxGen are to simplify and automate MACOS prescription generations, reducing errors associated with creating such optical prescriptions, and improving user efficiency without requiring MACOS proficiency. RxGen uses MATLAB (a high-level language and interactive environment developed by MathWorks) as the development and deployment platform, but RxGen can easily be ported to another optical modeling/analysis platform. Running RxGen within the modeling environment has the huge benefit that variations in optical models can be made an integral part of the modeling state. For instance, optical prescription parameters determined as external functional dependencies, optical variations by controlling the in-/exclusion of optical components like sub-systems, and/or controlling the state of all components. Combining the mentioned capabilities and flexibilities with RxGen's optical abstraction layer completely eliminates the hindering aspects for requiring proficiency in writing/editing MACOS prescriptions, allowing users to focus on the modeling aspects of optical systems, i.e., increasing productivity and efficiency. RxGen provides significant enhancements to MACOS and delivers a framework for fast prototyping as well as for developing very complex controlled optical systems.

  5. Downregulation of miR-139-5p contributes to the antiapoptotic effect of liraglutide on the diabetic rat pancreas and INS-1 cells by targeting IRS1.

    Science.gov (United States)

    Li, Jin; Su, Lei; Gong, Ying-Ying; Ding, Mei-Lin; Hong, Shu-Bin; Yu, Shuang; Xiao, Hai-Peng

    2017-01-01

    Liraglutide is administered as glucagon-like peptide-1 (GLP-1) receptor agonist for diabetic patients and can protect pancreatic β-cells by inhibiting their apoptosis. MicroRNA-139-5p (miRNA-139-5p) participates in the regulation of cancer cell apoptosis. However, it is not clear whether miR-139-5p contributes to the anti-apoptotic effect of liraglutide in β-cells. The objective of the present study was to investigate the role of miR-139-5p on apoptosis of pancreatic β-cells. MicroRNA levels in pancreatic tissue from diabetic rats and INS-1 cells treated with liraglutide were measured by real-time quantitative RT-PCR. The role of miR-139-5p on apoptosis was studied by transfecting INS-1 cells with miR-139-5p mimics. The mRNA and protein expression of the target gene, insulin receptor substrate-1 (IRS1), were measured by qRT-PCR and Western blot, respectively. Apoptosis in rat pancreatic tissue and INS-1 cells was detected by TUNEL and annexin V/propidium iodide costaining. Apoptosis of pancreatic tissue from diabetic rats and INS-1 cells was decreased by administration of liraglutide. The expression of miR-139-5p increased in the pancreas of diabetic rats and decreased with liraglutide treatment. Incubation with liraglutide (100 nM) for 48 h attenuated the expression of miR-139-5p and increased the mRNA and protein levels of IRS1. Direct regulatory effects of miR-139-5p on IRS1 were found by a dual-luciferase reporter assay. Transfection of INS-1 cells with miR-139-5p mimics led to decreases in the mRNA and protein expression of IRS1. In conclusion, our observations suggest that decreased miR-139-5p expression contributes to the anti-apoptotic effect of liraglutide on the diabetic rat pancreas and INS-1 cells by targeting IRS1.

  6. Modelo poblacional con algoritmos genéticos

    OpenAIRE

    Veliz Quintero, Eduardo; Rodriguez Ojeda, Luis

    2009-01-01

    Para el desarrollo de este trabajo, “MODELO POBLACIONAL CON ALGORITMOS GENÉTICOS”, he investigado la rama de la inteligencia artificial, como son los algoritmos genéticos. Primero presento en forma general los aspectos que envuelven los algoritmos genéticos, parto de la necesidad de optimizar, así como su historia y posibles aplicaciones y luego he cubierto detalladamente todo lo que pude investigar sobre la teoría de los algoritmos genéticos, sus fundamentos matemáticos, tipos de algoritmos ...

  7. Genética forense no-humana

    OpenAIRE

    Peral García, Pilar; Giovambattista, Guillermo; Ripoli, María Verónica

    2015-01-01

    El presente libro nace como producto de una convocatoria para Libros de Cátedra de la Universidad Nacional de la Plata. La propuesta, avalada por la Facultad de Ciencias Veterinarias, permitirá introducir al lector en los principales aspectos de la identificación genética de animales y/o sus productos derivados, conocer los principales métodos de genotipificación utilizados en genética forense, reconocer los lineamientos de estandarización y acreditación de los laboratorios de genética forens...

  8. GenBank blastx search result: AK062102 [KOME

    Lifescience Database Archive (English)

    Full Text Available transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608; aspartic

  9. GenBank blastx search result: AK242621 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  10. GenBank blastx search result: AK243655 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  11. EPCGen2 Pseudorandom Number Generators: Analysis of J3Gen

    Directory of Open Access Journals (Sweden)

    Alberto Peinado

    2014-04-01

    Full Text Available This paper analyzes the cryptographic security of J3Gen, a promising pseudo random number generator for low-cost passive Radio Frequency Identification (RFID tags. Although J3Gen has been shown to fulfill the randomness criteria set by the EPCglobal Gen2 standard and is intended for security applications, we describe here two cryptanalytic attacks that question its security claims: (i a probabilistic attack based on solving linear equation systems; and (ii a deterministic attack based on the decimation of the output sequence. Numerical results, supported by simulations, show that for the specific recommended values of the configurable parameters, a low number of intercepted output bits are enough to break J3Gen. We then make some recommendations that address these issues.

  12. Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigrus oceanense gen. nov., sp nov., Desulfofrigus fragile sp nov., Desulfofaba gelida gen. nov., sp nov., Desulfotalea psychrophila gen. nov., sp nov and Desulfotalea arctica sp

    DEFF Research Database (Denmark)

    Knoblauch, C.; Sahm, K.; Jørgensen, BB

    1999-01-01

    known sulfate reducers. Due to the phylogenetic and phenotypic differences between the new isolates and their closest relatives, establishment of the new genera Desulfotalea gen. nov., Desulfofaba gen. nov. and Desulfofrigus gen. nov. is proposed, with strain ASv26(T) as the type strain of the type...

  13. Equilibrium analysis in imperfect Traders' and GenCos' market

    OpenAIRE

    Zhong, J; Chitkara, P.

    2010-01-01

    The paper models the strategic behavior of traders, GenCos and ISO using the multi-leader-follower framework. The outcomes of the strategic behavior of the players have been modeled using an equilibrium problem with equilibrium constraints. From a policy perspective it is seen that allowing the GenCos to hold FTRs may be welfare enhancing under certain demand conditions and ownership patterns of transmission rights and generation assets. The proposed model has been simulated on a 3 bus system...

  14. Introducing AstroGen: The Astronomy Genealogy Project

    OpenAIRE

    Tenn, Joseph S.

    2016-01-01

    The Astronomy Genealogy Project ("AstroGen"), a project of the Historical Astronomy Division of the American Astronomical Society (AAS), will soon appear on the AAS website. Ultimately, it will list the world's astronomers with their highest degrees, theses for those who wrote them, academic advisors (supervisors), universities, and links to the astronomers or their obituaries, their theses when on-line, and more. At present the AstroGen team is working on those who earned doctorates with ast...

  15. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus)

    OpenAIRE

    Raden Roro Sri Pudji Sinarni Dewi; Alimuddin Alimuddin; Agus Oman Sudrajat; Komar Sumantadinata; Erma Primanita Hayuningtyas

    2016-01-01

    Penelitian ekspresi sementara (transient expression) dari transgen secara in vivo menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein). Pengamatan gen EGFP (enhanced green fluorescent protein) pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus) ditujukan untuk mendapatkan informasi mengenai kema...

  16. Banque Cantonale de Genève

    CERN Multimedia

    Banque Cantonale de Genève

    2011-01-01

    7e Salon Immobilier BCGE le samedi 3 septembre 2011, de 8 h 30 à 13 h 00, au Centre de formation de Conches À cette occasion, les meilleurs spécialistes professionnels genevois de l’immobilier seront réunis en un seul et même lieu. Si vous le souhaitez, un conseiller spécialisé dans les financements hypothécaires évaluera vos possibilités d’investissement immobilier adaptées à votre situation personnelle. En parallèle, les plus importantes régies immobilières de Genève seront à votre disposition pour vous présenter leurs offres actuelles, ainsi que les projets immobiliers futurs et discuter avec vous de la meilleure stratégie à adopter pour trouver l’objet de vos rêves. De plus, vous aurez la possibilité...

  17. Identidad y Eponimia genérica

    Directory of Open Access Journals (Sweden)

    Cristina Virgen Aldana-Zayas

    2013-01-01

    Full Text Available Se aborda el tema de la Eponimia genérica de la flora vascular cubana que comprende el nombre dado a cada género de las plantas superiores y su relación con la formación identitaria en los estudiantes. Para ello se realizó una revisión bibliográfica de los cinc o tomos de la flora cubana y Árboles de Cuba. Se tomó una muestra de 331 géneros. Además se utilizaron otras fuentes bibliográficas para establecer los nexos entre la lingüística, la identidad y los nombres de los géneros de la flora vascular cubana. El empleo del método histórico-lógico permitió constatar que la procedencia de los nombres de los géneros puede deberse a la mitología, a la geografía y a nombres de científicos. Para la investigación se seleccionaron los nombres de los géneros dedicados a personalidades de la ciencia. Se confeccionó un Catálogo que ofrece las herramientas necesarias para que profesores y estudiantes realicen sus propias indagaciones y lleguen a conclusiones sobre los epónimos y sus potencialidades identitarias.

  18. New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales).

    Science.gov (United States)

    Smith, Matthew E; Amses, Kevin R; Elliott, Todd F; Obase, Keisuke; Aime, M Catherine; Henkel, Terry W

    2015-12-01

    Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera.

  19. Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov.--two new middle Cambrian hyolithids (?Mollusca) from the Czech Republic.

    Science.gov (United States)

    Valent, Martin; Fatka, Oldřich; Szabad, Michal; Micka, Václav; Marek, Ladislav

    2015-08-28

    Two new endemic genera and species of extinct group of Hyolitha, Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov. are described and illustrated from the Buchava Formation of the Barrandian area (Czech Republic).

  20. A multiple system of high-mass YSOs surrounded by disks in NGC 7538 IRS1 . Gas dynamics on scales of 10-700 AU from CH3OH maser and NH3 thermal lines

    Science.gov (United States)

    Moscadelli, L.; Goddi, C.

    2014-06-01

    Context. It has been claimed that NGC 7538 IRS1 is a high-mass young stellar object (YSO) with 30 M⊙, surrounded by a rotating Keplerian disk, probed by a linear distribution of methanol masers. The YSO is also powering a strong compact Hii region or ionized wind, and is driving at least one molecular outflow. The axis orientations of the different structures (ionized gas, outflow, and disk) are, however, misaligned, which has led to the different competing models proposed to explain individual structures. Aims: We investigate the 3D kinematics and dynamics of circumstellar gas with very high linear resolution, from tens to 1500 AU, with the ultimate goal of building a comprehensive dynamical model for what is considered the best high-mass accretion disk candidate around an O-type young star in the northern hemisphere. Methods: We used high-angular resolution observations of 6.7 GHz CH3OH masers with the EVN, NH3 inversion lines with the JVLA B-Array, and radio continuum with the VLA A-Array. In particular, we employed four different observing epochs of EVN data at 6.7 GHz, spanning almost eight years, which enabled us to measure line-of-sight (l.o.s.) accelerations and proper motions of CH3OH masers, besides l.o.s. velocities and positions (as done in previous works). In addition, we imaged highly excited NH3 inversion lines, from (6,6) to (13,13), which enabled us to probe the hottest molecular gas very close to the exciting source(s). Results: We confirm previous results that five 6.7 GHz maser clusters (labeled from "A" to "E") are distributed over a region extended N-S across ≈1500 AU, and are associated with three components of the radio continuum emission. We propose that these maser clusters identify three individual high-mass YSOs in NGC 7538 IRS1, named IRS1a (associated with clusters "B" and "C"), IRS1b (associated with cluster "A"), and IRS1c (associated with cluster "E"). We find that the 6.7 GHz masers distribute along a line, with a regular

  1. The study on the relationship between IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms and type 2 diabetes in the Kurdish ethnic group in West Iran.

    Science.gov (United States)

    Haghani, Karimeh; Bakhtiyari, Salar

    2012-11-01

    An association between the IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms and type 2 diabetes mellitus (T2DM) in different ethnic groups is controversial. We aimed to identify the association of these polymorphisms with T2DM in the Kurdish ethnic group of Iran. Study groups included 336 T2DM and 341 normoglycemic subjects. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism. Genotypic and allelic frequencies were then evaluated. GR and RR genotypes of IRS-1 Gly972Arg variant gave a higher risk for T2DM (odds ratios [OR]=1.76 and OR=3.86, respectively). IRS-1 Gly972Arg polymorphism was found to be significantly associated with T2DM (OR=1.63) for the dominant model (GG vs. GR+RR). GD genotypes of the IRS-2 Gly1057Asp variant gave a higher risk for T2DM (OR=1.63). The dominant model analysis of the IRS-2 Gly1057Asp genotypes (GG vs. GD+DD) also showed an enhanced association with T2DM (OR=1.69). Among several combinations, GR/GD gave the highest risk for T2DM (OR=3.1). Other combinations were also significantly associated with T2DM, including, GR/GG (OR=1.86), RR/GG (OR=1.76), GG/GD (OR=1.83), and GG/DD (OR=2.35). HbA1c, serum triglyceride, and systolic blood pressure were higher in the control subjects with GR+RR genotypes compared with the GG genotype. Among the T2DM subjects, fasting plasma glucose was significantly lower in subjects with the GG genotype in relation to those with the GR+RR genotypes. Normoglycemic subjects carrying GD+DD genotypes of IRS-2 Gly1057Asp variation had a significantly higher fasting plasma glucose and total cholesterol, as compared with those with the GG genotype. Our findings revealed that IRS-1 Gly972Arg and IRS-2 Gly1057Asp polymorphisms are associated with T2DM in the Kurdish ethnic group.

  2. SEQ-GEN: A comprehensive multimission sequencing system

    Science.gov (United States)

    Salcedo, Jose; Starbird, Thomas J.

    1994-01-01

    SEQ-GEN is a user-interactive computer program used to plan and generate a sequence of commands for spacecraft. Desired activities are specified by the user of SEQ-GEN; SEQ-GEN in turn expands these activities, deriving the spacecraft commands necessary to accomplish the desired activities. SEQ-GEN models the effects on the spacecraft of the commands, predicting the state as a function of time, flagging any conflicts and rule violations. These states, conflicts, and violations are viewable both graphically and textually at the user's request. SEQ-GEN also displays the entire sequence graphically, showing each requested activity as a bar on its graphical timeline. SEQ-GEN immediately revalidates the sequence, updating its models and calculations along with its displays based on these changes. Because it has the ability to recalculate spacecraft states immediately, the user is able to perform 'what-if' sessions easily. SEQ-GEN, a multimission tool, is adaptable to any flight project. A flight project writes its adaptation files containing project unique information including in its simplest form, only spacecraft commands. For more involved projects the adaptation files may also contain flight and mission rules, description of the spacecraft and ground models, and the definition of activities. SEQ-GEN operates at whatever level of detail the adaptation files imply. Simple adaptations are straight forward to do. There is, however, no limit to the complexity of activity definitions or of spacecraft models: both may involve unlimited logical decision points. Commands and activities may involve any number of parameters of a wide variety of data types, including integer, float, time, boolean, and character strings. SEQ-GEN will be used by the Mars Pathfinder, Cassini, and VIM (Voyager Interstellar Mission) project in an effort to speed up adaptation time and to keep sequence generation costs down. SEQ-GEN is hosted on UNIX workstations. It uses MOTIF and X for windowing

  3. Enfoque genómico en la enfermedad cardiovascular

    Directory of Open Access Journals (Sweden)

    Ismael San Mauro-Martín

    Full Text Available Introducción: las enfermedades cardiovasculares (EC constituyen la principal causa de muerte a nivel mundial. La etiología es multifactorial, pueden influir diversos factores como la dieta, los hábitos de vida, el nivel de ejercicio físico o la carga genética. El gran número de genes implicados, así como sus diversas variantes, pueden influir sobre el riesgo de padecer enfermedades cardiovasculares por medio de distintas vías. Objetivo: determinar la relación existente entre diferentes polimorfismos genéticos y el riesgo individual de EC en población infantil y adulta. Métodos: se llevó a cabo una búsqueda bibliográfica utilizando la base de datos PubMed. La búsqueda se limitó a un periodo de diez años y a metaanálisis realizados en humanos. Resultados: se establece relación entre el riesgo de enfermedad cardiovascular y los siguientes polimorfismos genéticos: cromosoma 9p21, apolipoproteína A5, apolipoproteínas E2, E3 y E4, gen PPARG o PPARΥ, genes implicados en el metabolismo lipídico, gen MTHFR, citocromo P450, factor V de coagulación o factor de Leiden (FVL y gen VKORC. Conclusiones: Se han identificado un gran número de genes relacionados con la enfermedad cardiovascular. La carga genética puede influir de manera directa o indirecta sobre el riesgo cardiovascular, modificando factores de riesgo para enfermedad cardiovascular o actuando sobre la medicación empleada para tratarla.

  4. 12b-hydroxy-des-D-garcigerin A enhances glucose metabolism in insulin-resistant HepG2 cells via the IRS-1/PI3-K/Akt cell signaling pathway.

    Science.gov (United States)

    Chen, Yu; Wang, Sha; Tian, Shi-Ting; Hu, Xin; Xu, Jing; Yang, Guang-Zhong; Wang, Chao-Yuan

    2016-11-01

    HepG2 cells were induced with a high concentration of insulin to establish an insulin-resistant cell model (HepG2/IR). The effect of 12b-hydroxy-des-D-garcigerin A (DGA) on the glucose consumption (GC) of HepG2/IR cells was analyzed with the glucose oxidase/peroxidase assay. The results showed that DGA significantly stimulated GC by enhancing the activity of hexokinase (HK) and pyruvate kinase (PK) in HepG2/IR cells. The cell signaling pathway by which DGA enhances the GC of HepG2/IR cells was explored. The results showed that DGA promoted the expression of insulin receptor (InsR) protein, and stimulated the expression of insulin receptor substrate 1 (IRS-1), phosphatidylinositol-3 kinase (p-PI3-K), and phospho-protein kinase B Serine(473) (p-AKT ser(473)). Therefore, we concluded that DGA improved the insulin-resistance of HepG2/IR cells by inducing the IRS-1/PI3-K/Akt cell signaling pathway. Interestingly, DGA had no effect on the phosphorylation of threonine(172) (Thr(172)) in AMP-activated protein kinase (AMPK).

  5. ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity.

    Science.gov (United States)

    Zhao, Jie; Wang, Min; Deng, Wuquan; Zhong, Daping; Jiang, Youzhao; Liao, Yong; Chen, Bing; Zhang, Xiaoli

    2017-05-13

    Decreased phosphorylation in the insulin signalling pathway is a hallmark of insulin resistance. The causes of this phenomenon are complicated and multifactorial. Recently, genomic analyses have identified ARL15 as a new candidate gene related to diabetes. However, the ARL15 protein function remains unclear. Here, we show that ARL15 is upregulated by insulin stimulation. This effect was impaired in insulin-resistant pathophysiology in TNF-α-treated C2C12 myotubes and in the skeletal muscles of leptin knockout mice. In addition, ARL15 localized to the cytoplasm in the resting state and accumulated in the Golgi apparatus around the nucleus upon insulin stimulation. ARL15 overexpression can enhance the phosphorylation of the key insulin signalling pathway molecules IR, IRS1 and AKT in C2C12 myotubes. Moreover, ARL15 knockdown can also specifically inhibit the phosphorylation of PDPK1 Ser241, thereby reducing PDPK1 activity and its downstream phosphorylation of AKT Thr308. Co-immunoprecipitation assays identified ASAP2 as an ARL15-interacting protein. In conclusion, we have identified that ARL15 acts as an insulin-sensitizing effector molecule to upregulate the phosphorylation of members of the canonical IR/IRS1/PDPK1/AKT insulin pathway by interacting with its GAP ASAP2 and activating PDPK1. This research may provide new insights into GTPase-mediated insulin signalling regulation and facilitate the development of new pharmacotherapeutic targets for insulin sensitization. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Ursolic acid and rosiglitazone combination improves insulin sensitivity by increasing the skeletal muscle insulin-stimulated IRS-1 tyrosine phosphorylation in high-fat diet-fed C57BL/6J mice.

    Science.gov (United States)

    Sundaresan, Arjunan; Radhiga, Thangaiyan; Pugalendi, Kodukkur Viswanathan

    2016-06-01

    The aim of this present study was to investigate the effect of ursolic acid (UA) and rosiglitazone (RSG) on insulin sensitivity and proximal insulin signaling pathways in high-fat diet (HFD)-fed C57/BL/6J mice. Male C57BL/6J mice were fed either normal diet or HFD for 10 weeks, after which animals in each dietary group were divided into the following six groups (normal diet, normal diet plus UA and RSG, HFD alone, HFD plus UA, HFD plus RSG, and HFD plus UA and RSG) for the next 5 weeks. UA (5 mg/kg BW) and RSG (4 mg/kg BW) were administered as suspensions directly into the stomach using a gastric tube. The HFD diet elevated fasting plasma glucose, insulin, and homeostasis model assessment index. The expression of insulin receptor substrate (IRS)-1, phosphoinositide 3-kinase (PI3-kinase), Akt, and glucose transporter (GLUT) 4 were determined by Western blot analyses. The results demonstrated that combination treatment (UA/RSG) ameliorated HFD-induced glucose intolerance and insulin resistance by improving the homeostatic model assessment (HOMA) index. Further, combination treatment (UA/RSG) stimulated the IRS-1, PI3-kinase, Akt, and GLUT 4 translocation. These results strongly suggest that combination treatment (UA/RSG) activates IRS-PI3-kinase-Akt-dependent signaling pathways to induce GLUT 4 translocation and increases the expression of insulin receptor to improve glucose intolerance.

  7. ANALISIS GEN HAEMAGGLUTININ PADA VIRUS CAMPAK LIAR

    Directory of Open Access Journals (Sweden)

    Subangkit Subangkit

    2015-05-01

    Full Text Available AbstrakPenyakit Campak disebabkan oleh virus campak yang termasuk genus Morbilivirus dan Family Paramyxoviridae. Penyakit campak masih menjadi masalah kesehatan karena masih ditemukan Kejadian Luar Biasa (KLB di Indonesia. Salah satu penyebab terjadinya KLB tersebut diduga sebagaiakibat perbedaan antigenesitas antara strain vaksin yang digunakan dengan strain virus campak liar yang beredar di Indonesia. Penelitian ini bertujuan mendapatkan gambaran tentang karakteristik genetik gen Haemagglutinin virus campak liar yang ada di Indonesia. Spesimen yang digunakan sebanyak 27 isolat virus penyebab KLB dari 17 propinsi selama periode tahun 2003-2010. Isolat virus dilakukan pemeriksaan secara RT-PCR dan sekuensing dengan metode Sanger. Hasil sekuensing dianalisis dengan menggunakan perangkat lunak Bioedit 7.0 dan MEGA 4.0. Hasil penelitian didapatkan perbedaan 10 asam amino antara virus campak strain vaksin CAM-70 dan virus campak liar pada posisi D416N; K424T; V451M; N455T; V466I; I473T; F476L; Y481S atau Y481N; H495N; G505D. Kesimpulan penelitian ini adalah terdapat perbedaan karakteristik genetik antara virus campak liar di Indonesia berbeda dengan strain virus vaksin CAM-70.Kata kunci : Campak, Analisis Molekuler, Hemagglutinin, CD46AbstractMeasles is caused by virus belonging to the genus Morbilivirus and Family Paramyxoviridae. Measles is still a public health problem because outbreak of measles still found in Indonesia. Outbreak is suspected as a result of differences in antigenicity between vaccine strains used with wild-type measles virus strains circulating in Indonesia. This study aims to get genetic characteristics of wild-type measles virus haemagglutinin gene in Indonesia. The specimens were used 27 viral isolates from 17 provinces period 2003-2010. Viral isolates examined by RT-PCR and sequencing with Sanger method. Sequencing analysis were conducted using Bioedit 7.0 and MEGA 4.0 software. The results showed 10 amino acid differences

  8. Genética e hanseníase

    Directory of Open Access Journals (Sweden)

    Bernardo Beiguelman

    Full Text Available As diferentes linhas de pesquisa utilizadas para investigar a importância dos fatores hereditários humanos na determinação da resistência/suscetibilidade à infecção pelo Mycobacterium leprae foram discutidas no presente trabalho. Uma síntese dessas abordagens permitiu analisar os resultados das investigações sobre associação da hanseníase com polimorfismos genéticos, distribuição familial da hanseníase, prevalência da hanseníase e distância genética, concordância da hanseníase em gêmeos e estudos genéticos sobre a reação de Mitsuda.

  9. A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity.

    Directory of Open Access Journals (Sweden)

    Min Gao

    Full Text Available The maintenance of genomic stability requires accurate genome replication, repair of DNA damage, and the precise segregation of chromosomes in mitosis. GEN1 possesses Holliday junction resolvase activity in vitro and presumably functions in homology driven repair of DNA double strand breaks. However, little is currently known about the cellular functions of human GEN1. In the present study we demonstrate that GEN1 is a novel centrosome associated protein and we characterize the various phenotypes associated with GEN1 deficiency. We identify an N-terminal centrosome localization signal in GEN1, which is required and sufficient for centrosome localization. We report that GEN1 depletion results in aberrant centrosome numbers associated with the formation of multiple spindle poles in mitosis, an increased number of cells with multi-nuclei, increased apoptosis and an elevated level of spontaneous DNA damage. We find homologous recombination severely impaired in GEN1 deficient cells, suggesting that GEN1 functions as a Holliday junction resolvase in vivo as well as in vitro. Complementation of GEN1 depleted cells with various GEN1 constructs revealed that centrosome association but not catalytic activity of GEN1 is required for preventing centrosome hyper-amplification, formation of multiple mitotic spindles, and multi-nucleation. Our findings provide novel insight into the biological functions of GEN1 by uncovering an important role of GEN1 in the regulation of centrosome integrity.

  10. La genética de las poblaciones centroamericanas

    Directory of Open Access Journals (Sweden)

    Barrantes, Ramiro

    2005-01-01

    Full Text Available Las poblaciones centroamericanas no han sido objeto de muchos estudios genéticos con la excepción de análisis esporádicos de la variación entre y dentro de los grupos amerindios y de origen africano ubicados en el área. No obstante, en los últimos 15 años se efectuaron investigaciones sistemáticas en este sentido incluyendo poblaciones mestizas, particularmente las de Costa Rica y Panamá. En los amerindios se efectuaron estudios detallados de su estructura genética y las relaciones filogenéticas entre poblaciones en un contexto regional y continental. De manera general estos grupos mantienen una baja diversidad genética. Por otra parte, estimativas de la mezcla génica en la población mestiza muestran diferencias dentro y entre los distintos países del área. Al Valle Central de Costa Rica se le atribuye la condición de aislado con una supuesta constitución genética homogénea favorable para los estudios de desequilibrio de ligamiento y asociaciones con algunas enfermedades hereditarias; sin embargo, los resultados obtenidos con relación el flujo génico direccional, la amalgama de etnias y otros componentes de su estructura como la variación temporal y espacial de la consanguinidad y la migración indican una mayor heterogeneidad. Se muestran los resultados y conclusiones obtenidas y se plantean las perspectivas y tendencias al mediano plazo que vinculan los enfoques y métodos de la epidemiología y la antropología genética y el estudio genético de poblaciones.

  11. Craniostenose em gêmeos: estudo genético

    Directory of Open Access Journals (Sweden)

    Walter Carlos Pereira

    1968-09-01

    Full Text Available É relatada a ocorrência de formas clínicas diversas de craniostenose em gêmeos de sexo diferente. A menina apresentava obliteração completa da sutura coronaria e dos dois terços anteriores da sutura sagital; no menino a sutura sagital era a única afetada. O estudo genético mostrou que a craniostenose independe de aberrações cromossômicas, indicando ser transmitida por gens recessivos raros de natureza autossômica.

  12. Biotecnologia aplicada ao melhoramento genético do cafeeiro

    OpenAIRE

    2011-01-01

    O melhoramento genético do cafeeiro mediante técnicas convencionais é trabalhoso e demorado. A biotecnologia oferece estratégias alternativas para auxiliar na multiplicação e no desenvolvimento de novas variedades com resistência a estresses bióticos e abióticos, melhor qualidade de bebida e maturação mais uniforme dos frutos. As técnicas de cultura de tecidos têm possibilitado a obtenção de grande número de plantas e a garantia da uniformidade genética do material. O emprego de marcadores mo...

  13. Introducing AstroGen: the Astronomy Genealogy Project

    Science.gov (United States)

    Tenn, Joseph S.

    2016-12-01

    The Astronomy Genealogy Project (AstroGen), a project of the Historical Astronomy Division of the American Astronomical Society (AAS), will soon appear on the AAS website. Ultimately, it will list the world's astronomers with their highest degrees, theses for those who wrote them, academic advisors (supervisors), universities, and links to the astronomers or their obituaries, their theses when online, and more. At present the AstroGen team is working on those who earned doctorates with astronomy-related theses. We show what can be learned already, with just ten countries essentially completed.

  14. Genética, saúde e sociedade

    OpenAIRE

    2013-01-01

    O documentário apresenta quatro projetos de pesquisa realizados pelo Instituto de GenéticaMédica Populacional (INAGEMP) em diferentes regiões do país.As atividades mencionadasrepresentam uma amostra do trabalho do INAGEMP, que se estruturou de modo a colocar agenética médica populacional na prática ao associar pesquisa e atenção em saúde parapopulações afetadas por doenças com forte componente genético em sua etiologia.

  15. Rutaecarpine ameliorates hyperlipidemia and hyperglycemia in fat-fed, streptozotocin-treated rats via regulating the IRS-1/PI3K/Akt and AMPK/ACC2 signaling pathways.

    Science.gov (United States)

    Nie, Xu-qiang; Chen, Huai-hong; Zhang, Jian-yong; Zhang, Yu-jing; Yang, Jian-wen; Pan, Hui-jun; Song, Wen-xia; Murad, Ferid; He, Yu-qi; Bian, Ka

    2016-04-01

    We have shown that rutaecarpine extracted from the dried fruit of Chinese herb Evodia rutaecarpa (Juss) Benth (Wu Zhu Yu) promotes glucose consumption and anti-inflammatory cytokine expression in insulin-resistant primary skeletal muscle cells. In this study we investigated whether rutaecarpine ameliorated the obesity profiles, lipid abnormality, glucose metabolism and insulin resistance in rat model of hyperlipidemia and hyperglycemia. Rats fed on a high-fat diet for 8 weeks, followed by injection of streptozotocin (30 mg/kg, ip) to induce hyperlipidemia and hyperglycemia. One week after streptozotocin injection, the fat-fed, streptozotocin-treated rats were orally treated with rutaecarpine (25 mg·kg(-1)·d(-1)) or a positive control drug metformin (250 mg·kg(-1)·d(-1)) for 7 weeks. The body weight, visceral fat, blood lipid profiles and glucose levels, insulin sensitivity were measured. Serum levels of inflammatory cytokines were analyzed. IRS-1 and Akt/PKB phosphorylation, PI3K and NF-κB protein levels in liver tissues were assessed; pathological changes of livers and pancreases were examined. Glucose uptake and AMPK/ACC2 phosphorylation were studied in cultured rat skeletal muscle cells in vitro. Administration of rutaecarpine or metformin significantly decreased obesity, visceral fat accumulation, water consumption, and serum TC, TG and LDL-cholesterol levels in fat-fed, streptozotocin-treated rats. The two drugs also attenuated hyperglycemia and enhanced insulin sensitivity. Moreover, the two drugs significantly decreased NF-κB protein levels in liver tissues and plasma TNF-α, IL-6, CRP and MCP-1 levels, and ameliorated the pathological changes in livers and pancreases. In addition, the two drugs increased PI3K p85 subunit levels and Akt/PKB phosphorylation, but decreased IRS-1 phosphorylation in liver tissues. Treatment of cultured skeletal muscle cells with rutaecarpine (20-180 μmol/L) or metformin (20 μmol/L) promoted the phosphorylation of AMPK

  16. Divergência genética em genótipos de girassol Genetic divergence in sunflower genotypes

    Directory of Open Access Journals (Sweden)

    Edson Perito Amorim

    2007-12-01

    Full Text Available Uma investigação sobre a diversidade genética entre 15 genótipos de girassol, por meio de 12 características agronômicas, foi implementada no Instituto Agronômico, Campinas, Brasil. Análises de variância univariada e multivariada revelaram diferenças entre os genótipos. A distância generalizada de Mahalanobis indicou um alto grau de divergência genética. Os genótipos foram agrupados em três grupos. As características início do florescimento, 50% do florescimento, número de folhas e altura da inserção do capítulo contribuíram com grande parte da divergência genética observada. Por meio desses resultados, é possível identificar materiais divergentes e com características agronômicas complementares para o desenvolvimento de novos cultivares superiores.An investigation about the genetical diversity among fifteen sunflower genotypes using twelve agronomical characteristics was implanted at the Agronomic Institute, Campinas Brazil. Univariate and multivariate analyses of variance revealed the presence of differences among the genotypes. The generalized distance of Mahalanobis indicated the presence of genetic diversity. The genotypes were grouped into tree clusters. Among the investigated characteristics, the beginning of flowering, 50% flowering, leaf number and head height of chapter insertion exhibited high contribution towards genetic divergence. Through these studies it is possible to identify divergent material with further agronomical features for the development of new superior sunflower cultivars.

  17. Synthesis, structural, spectral (FT-IR, 1H and 13C NMR and UV-Vis), NBO and first order hyperpolarizability analysis of N-(4-nitrophenyl)-2, 2-dibenzoylacetamide by density functional theory

    Science.gov (United States)

    Yalçın, Şerife Pınar; Ceylan, Ümit; Sarıoğlu, Ahmet Oral; Sönmez, Mehmet; Aygün, Muhittin

    2015-10-01

    The title compound, C22H16N2O5, was synthesized and characterized by experimental techniques (FT-IR, 1H NMR, 13C NMR, UV-Vis and X-Ray single crystal determination) and theoretical calculations. The molecular geometry, vibrational frequencies, molecular electrostatic potential (MEP), thermodynamic properties, the dipole moments, HOMO-LUMO energy has been calculated by using the Density Functional Theory (DFT) method with 6-311G(d,p) and 6-311++G(d,p) basis sets. 1H and 13C NMR chemical shifts show good agreement with experimental values. According to calculated results, the 6-311G(d,p) and 6-311++G(d,p) basis sets have showed similar results. The optimized geometry can well reproduce the crystal structure parameters.

  18. A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors.

    Energy Technology Data Exchange (ETDEWEB)

    Rizwan-uddin; Nick Karancevic; Stefano Markidis; Joel Dixon; Cheng Luo; Jared Reynolds

    2008-04-23

    many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important crterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals.

  19. Epidemiologia genética: epidemiologia, genética ou nenhuma das anteriores?

    Directory of Open Access Journals (Sweden)

    Aguinaldo Gonçalves

    1990-12-01

    Full Text Available No esforço de contribuir para melhor entendimento da identidade da Epidemiologia Genética, são revistas sua concepção, campo de atuação, métodos e técnicas pertinentes e algumas instâncias de aplicação. Entendendo-a como a área de interesse dos fatores genéticos das doenças e suas interações ambientais, apresenta-se seu campo de atuação como constituído por dois segmentos: um descritivo, que lida com conhecimento da distribuição de tais afecções em famílias e populações, seu impacto a nível do coletivo e sua vigilância epidemiológica, bem como o estudo de seus determinantes; o segundo, caracterizado pela intervenção, refere-se às respectivas medidas preventivas. Em que pese possível limitação pela não-consideração de todas as situações existentes, particular atenção é destinada à revisão de métodos e técnicas que possam ser convergentemente aplicados, a partir de procedimentos genéticos e epidemiológicos. Entre eles, destacam-se como estudos de casos tanto metodologias laboratoriais (como os dermatóglifos quanto quantitativos, como cálculo de herdabilidade e análise multivariada. Alguns objetos de estudo são tomados como instância de aplicação, por contarem com investigações específicas em nosso meio: a hanseníase, o hidrargirismo e a esquizofrenia.In an attempt to contribute to a better undestanding of the identity of Genetic Epidemiology, we review its conception, its field of influence, its appropriate methods and techniques and, at last, some of its applications. Genetic Epidemiology involves the study of genetic factors acting on diseases and on their environmental interactions. These includes two major areas: a descriptive one, related to the distribution of such conditions in families and populations, to the epidemiologic surveillance and to the study of determinants; and another characterized by intervention, which is related to preventive measures. Because of the dificulty in

  20. A Novel Role of Human Holliday Junction Resolvase GEN1 in the Maintenance of Centrosome Integrity

    DEFF Research Database (Denmark)

    Gao, M.; Danielsen, Jannie Michaela Rendtlew; Wei, L.-Z.;

    2012-01-01

    but not catalytic activity of GEN1 is required for preventing centrosome hyper-amplification, formation of multiple mitotic spindles, and multi-nucleation. Our findings provide novel insight into the biological functions of GEN1 by uncovering an important role of GEN1 in the regulation of centrosome integrity...

  1. 78 FR 28940 - Ninth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-05-16

    ... Federal Aviation Administration Ninth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held June 4, 2013 from...

  2. 78 FR 5860 - Eighth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-01-28

    ... Federal Aviation Administration Eighth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... meeting of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held February 7,...

  3. 78 FR 54509 - Tenth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-09-04

    ... Federal Aviation Administration Tenth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held September 19, 2013...

  4. Mapeo genético en camarón blanco litopenaeus vannamei

    OpenAIRE

    Erazo Maldonado, Edna Constanza

    2002-01-01

    Mapeo genético en camarón blanco Litopenaeus vannamei Se presenta el primer mapa genético de Litopenaeus vannamei basado en la técnica de AFLPs a partir de una familia proveniente de un cruce comercial y con la información genética de un solo progenitor y 42 hijos.

  5. Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates.

    Science.gov (United States)

    Bellendir, Stephanie P; Rognstad, Danielle J; Morris, Lydia P; Zapotoczny, Grzegorz; Walton, William G; Redinbo, Matthew R; Ramsden, Dale A; Sekelsky, Jeff; Erie, Dorothy A

    2017-05-19

    Human GEN1 and yeast Yen1 are endonucleases with the ability to cleave Holliday junctions (HJs), which are proposed intermediates in recombination. In vivo, GEN1 and Yen1 function secondarily to Mus81, which has weak activity on intact HJs. We show that the genetic relationship is reversed in Drosophila, with Gen mutants having more severe defects than mus81 mutants. In vitro, DmGen, like HsGEN1, efficiently cleaves HJs, 5΄ flaps, splayed arms, and replication fork structures. We find that the cleavage rates for 5΄ flaps are significantly higher than those for HJs for both DmGen and HsGEN1, even in vast excess of enzyme over substrate. Kinetic studies suggest that the difference in cleavage rates results from a slow, rate-limiting conformational change prior to HJ cleavage: formation of a productive dimer on the HJ. Despite the stark difference in vivo that Drosophila uses Gen over Mus81 and humans use MUS81 over GEN1, we find the in vitro activities of DmGen and HsGEN1 to be strikingly similar. These findings suggest that simpler branched structures may be more important substrates for Gen orthologs in vivo, and highlight the utility of using the Drosophila model system to further understand these enzymes. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  6. GenBank blastx search result: AK058467 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 1p34.2-36.11 Contains the 3' end of the KPNA6 gene for karyopherin alpha 6 (importin alpha 7), a novel g...ene (DKFZp451J0118), a novel gene (MGC1203) a novel gene, a novel gene (FLJ10547), a novel gene, a novel gen

  7. Justicia y genética: compensando las diferencias

    Directory of Open Access Journals (Sweden)

    Alejandra Zúñiga-Fajuri

    2013-01-01

    Full Text Available Se analizan los dilemas morales asociados a los avances científicos que en la actualidad nos exigen repensar el concepto de igualdad equitativa de oportunidades. Asimismo, se pasa revista a la discusión filosófica en torno al origen de las desventajas sociales y genéticas que permiten las desigualdades sociales.

  8. Safer Systems: A NextGen Aviation Safety Strategic Goal

    Science.gov (United States)

    Darr, Stephen T.; Ricks, Wendell R.; Lemos, Katherine A.

    2008-01-01

    The Joint Planning and Development Office (JPDO), is charged by Congress with developing the concepts and plans for the Next Generation Air Transportation System (NextGen). The National Aviation Safety Strategic Plan (NASSP), developed by the Safety Working Group of the JPDO, focuses on establishing the goals, objectives, and strategies needed to realize the safety objectives of the NextGen Integrated Plan. The three goal areas of the NASSP are Safer Practices, Safer Systems, and Safer Worldwide. Safer Practices emphasizes an integrated, systematic approach to safety risk management through implementation of formalized Safety Management Systems (SMS) that incorporate safety data analysis processes, and the enhancement of methods for ensuring safety is an inherent characteristic of NextGen. Safer Systems emphasizes implementation of safety-enhancing technologies, which will improve safety for human-centered interfaces and enhance the safety of airborne and ground-based systems. Safer Worldwide encourages coordinating the adoption of the safer practices and safer systems technologies, policies and procedures worldwide, such that the maximum level of safety is achieved across air transportation system boundaries. This paper introduces the NASSP and its development, and focuses on the Safer Systems elements of the NASSP, which incorporates three objectives for NextGen systems: 1) provide risk reducing system interfaces, 2) provide safety enhancements for airborne systems, and 3) provide safety enhancements for ground-based systems. The goal of this paper is to expose avionics and air traffic management system developers to NASSP objectives and Safer Systems strategies.

  9. Distributed Generation Market Demand Model (dGen): Documentation

    Energy Technology Data Exchange (ETDEWEB)

    Sigrin, Benjamin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Gleason, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States); Preus, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States); Baring-Gould, Ian [National Renewable Energy Lab. (NREL), Golden, CO (United States); Margolis, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-02-01

    The Distributed Generation Market Demand model (dGen) is a geospatially rich, bottom-up, market-penetration model that simulates the potential adoption of distributed energy resources (DERs) for residential, commercial, and industrial entities in the continental United States through 2050. The National Renewable Energy Laboratory (NREL) developed dGen to analyze the key factors that will affect future market demand for distributed solar, wind, storage, and other DER technologies in the United States. The new model builds off, extends, and replaces NREL's SolarDS model (Denholm et al. 2009a), which simulates the market penetration of distributed PV only. Unlike the SolarDS model, dGen can model various DER technologies under one platform--it currently can simulate the adoption of distributed solar (the dSolar module) and distributed wind (the dWind module) and link with the ReEDS capacity expansion model (Appendix C). The underlying algorithms and datasets in dGen, which improve the representation of customer decision making as well as the spatial resolution of analyses (Figure ES-1), also are improvements over SolarDS.

  10. GenOVa: a computer program to generate orientational variants

    OpenAIRE

    Cayron, Cyril

    2007-01-01

    A computer program called GenOVa, written in Python, calculates the orientational variants, the operators (special types of misorientations between variants) and the composition table associated with a groupoid structure. The variants can be represented by three-dimensional shapes or by pole figures.

  11. A New Parent Generation: Meet Mr. and Mrs. Gen X

    Science.gov (United States)

    Howe, Neil

    2010-01-01

    Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. Gen-X parents and Boomer parents belong to two neighboring generations, each possessing its own location in history and its own peer personality. They are similar in some respects, but clearly different in…

  12. Meet Mr. and Mrs. Gen X: A New Parent Generation

    Science.gov (United States)

    Howe, Neil

    2010-01-01

    Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. In the early 1990s, Gen Xers began joining parent-teacher associations in the nation's elementary schools. Around 2005, they became the majority of middle school parents. By the fall of 2008, they took over…

  13. GenBank blastx search result: AK241821 [KOME

    Lifescience Database Archive (English)

    Full Text Available e for comparative gene identification transcript 94 (CGI-94), complete sequence. PRI 5e-40 1 ... ...3A3 gene for 60kDa splicing factor 3a subunit 3, the FHL3 gene for four and a half LIM domains 3 and the gen

  14. GenBank blastx search result: AK241192 [KOME

    Lifescience Database Archive (English)

    Full Text Available e for comparative gene identification transcript 94 (CGI-94), complete sequence. PRI 1e-114 1 ... ...3A3 gene for 60kDa splicing factor 3a subunit 3, the FHL3 gene for four and a half LIM domains 3 and the gen

  15. Boomers, Gen-Xers, and Millennials: Understanding the "New Students."

    Science.gov (United States)

    Oblinger, Diana

    2003-01-01

    Describes characteristics of the "new" college student, who may be an adult learner from the Baby Boomer era, a high school member of the "Millennial" generation, or a "Gen-Xer." Explores the learning styles of each type of student and discusses the importance of technology to each group. (SLD)

  16. GenBank blastn search result: AK064582 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK064582 002-112-F03 AY007820.1 Daucus carota ATPase8 (ATP8) gene, ATP8-Sp1b allele, complete cds; chimeric... ATPase9 (ATP9) gene, ATP9-Sp3 allele, complete cds; and chimeric ATPase6 (ATP6) gen

  17. Le CERN, chance ou risque pour Genève

    CERN Document Server

    Maiani, Luciano; Wenninger, Horst; CERN. Geneva

    1998-01-01

    Le CERN, Chance ou Risque pour Gen?ve? D?bat public contradictoire organis? avec le soutien de la Tribune de Gen?ve Lieu: Salle II, CICG,15 Rue de Varemb?. Date: Lundi 19 octobre 1998, 20h00 D?roulement de la soir?e: 1. Ouverture M. Ren? KÏchlin, Pr?sident du Grand Conseil M. Luciano Maiani, Directeur g?n?ral d?sign? du CERN 2. Introduction: Un regard critique sur le CERN (? d?signer) Le projet LHC (Manfred Buhler Broglin CERN) 3. D?bat th?matique men? par le mod?rateur (Marco Cattaneo, r?dacteur en chef de la Tribune de Gen?ve) entre trois representants de lÕopinion publique (Ren? Longet, d?put? socialiste; Yves de Pr?ville, physicien; Jacques Mirenowicz, chercheur) et trois repr?sentants du Cern (Horst Wenninger, Directeur de la Recherche/Technique, CERN; Manfred Buhler Broglin, Administra-teur du projet LHC, CERN; Maurice Bourquin, Professeur de Physique ? l'Universit? de Gen?ve et D?l?gu? de la Suisse au Conseil du CERN). Les th?mes abord?s seront: Ð A quoi sert le CERN? Ð Quel est lÕimpact ?conomiqu...

  18. GenSVM: a generalized multiclass support vector machine

    NARCIS (Netherlands)

    G.J.J. van den Burg (Gerrit); P.J.F. Groenen (Patrick)

    2016-01-01

    textabstractTraditional extensions of the binary support vector machine (SVM) to multiclass problems are either heuristics or require solving a large dual optimization problem. Here, a generalized multiclass SVM is proposed called GenSVM. In this method classification boundaries for a K-class proble

  19. Carinichremylus gen. nov. (Hymenoptera: Braconidae: Pambolinae) from Peru

    NARCIS (Netherlands)

    Achterberg, van C.

    1999-01-01

    A new genus of the subfamily Pambolinae (Braconidae) is reported from Peru (Carinichremylus gen. nov.; type species: Carinichremylus peleopodae spec. nov.), illustrated and described. The type species has been reared from a Peleopoda spec. (Lepidoptera: Oecophoridae). The new genus is closely

  20. Delineamento de experimentos em genética genômica Experimental design in genetical genomics

    Directory of Open Access Journals (Sweden)

    Guilherme Jordão de Magalhães Rosa

    2007-07-01

    Full Text Available Genética genômica é um termo utilizado para representar o estudo de processos genéticos controladores de caracteres fenotípicos de herança complexa, a partir da análise conjunta de informação relativa a fenótipos, estruturas de parentesco, marcadores moleculares e expressão gênica. Estudos de genética genômica são utilizados, por exemplo, para a estimação da herdabilidade de níveis de transcrição, para o mapeamento de locos controladores da expressao gênica (eQTL, do inglês expression Quantitative Trait Loci, e para o estudo de redes regulatórias. Genética genômica geralmente envolve experimentos com microarrays, os quais são ainda bastante caros e trabalhosos, limitando o tamanho amostral e conseqüentemente o poder estatístico de tais estudos. Desta maneira, é essencial que tais experimentos sejam otimizados do ponto de vista do delineamento, a partir de criteriosa escolha das amostras (indivíduos a serem utilizadas, e do controle rigoroso dos vários fatores que podem afetar as variáveis-resposta de interesse. Outro ponto fundamental na condução de tais experimentos refere-se à marcação das amostras de mRNA com os fluoróforos e ao pareamento das mesmas em cada lâmina de microarray, os quais devem ser cuidadosamente planejados para que não haja confundimento entre estes efeitos e os fatores biológicos de interesse. Nesta apresentação serão discutidas algumas estratégias para o planejamento de estudos de genética genômica, incluindo a seleção de indivíduos objetivando-se a maximização da dissimilaridade genética ou do número de eventos de recombinação, bem como a condução eficiente dos ensaios com microarrays para diferentes objetivos experimentais.Genetical genomics experiments combine information on phenotypic traits, molecular markers and gene expression to study the genetic mechanisms governing variation in complex traits. Such studies can be used, for example, to estimate

  1. DOE/NNSA perspective safeguard by design: GEN III/III+ light water reactors and beyond

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Paul Y [Los Alamos National Laboratory

    2010-12-10

    An overview of key issues relevant to safeguards by design (SBD) for GEN III/IV nuclear reactors is provided. Lessons learned from construction of typical GEN III+ water reactors with respect to SBD are highlighted. Details of SBD for safeguards guidance development for GEN III/III+ light water reactors are developed and reported. This paper also identifies technical challenges to extend SBD including proliferation resistance methodologies to other GEN III/III+ reactors (except HWRs) and GEN IV reactors because of their immaturity in designs.

  2. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus

    Directory of Open Access Journals (Sweden)

    Raden Roro Sri Pudji Sinarni Dewi

    2016-04-01

    menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein. Pengamatan gen EGFP (enhanced green fluorescent protein pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus ditujukan untuk mendapatkan informasi mengenai kemampuan promoter -aktin ikan mas dalam mengendalikan ekspresi gen EGFP. Gen EGFP diintroduksikan ke dalam sperma ikan patin siam menggunakan metode elektroporasi. Sperma yang telah dielektroporasi digunakan untuk membuahi sel telur ikan patin siam. Pengamatan ekspresi gen EGFP dilakukan setiap enam jam dimulai dari embrio fase 2 sel sampai larva. Berdasarkan hasil penelitian, gen EGFP terekspresi pada fase embrio dan larva ikan patin siam. Puncak ekspresi gen EGFP terjadi pada fase neurula dan menurun pada fase larva. Berdasarkan penelitian ini maka ikan patin siam transgenik telah berhasil dibentuk dan promoter -aktin ikan mas terbukti aktif dalam mengarahkan ekspresi gen asing (GFP di dalam tubuh ikan patin siam.

  3. Justicia en salud y genética

    Directory of Open Access Journals (Sweden)

    Maria Graciela De Ortuzar

    2014-06-01

    Full Text Available Las expectativas puestas en el conocimiento genético exceden el ámbito de la medicina tradiciona, debido a que la intervención directa en la lotería natural demandaría el replanteamiento de conceptos centrales de justicia en salud: necesidades médicas, enfermedad, normalidad, e igualdad de oportunidades en el acceso a la salud. El punto en debate es sí el replanteo de dichos conceptos conlleva un cambio radical en las teorías de justicia (libertariana y/o liberal, mostrando su obsolescencia, o sí simplemente se requiere ampliar dichos conceptos claves por fallas estructurales en las mismas teorías. Como hipótesis general considero que los supuestos cuestionamientos, lejos de socavar las bases de las teorías de justicia, sólo ponen en evidencia sus viejos problemas estructurales. Por razones expositivas, dividiré la presentación tres partes. En la Primera parte, analizo la teoría libertariana, estudiando las contradicciones del modelo a través del impacto de la información genética en el seguro privado de salud. En la Segunda Parte, desarrollo la propuesta alternativa liberal rawlsianadanielsiana del modelo de seguro público, evaluando las implicaciones de la genética a partir de la crítica de su concepto biológico de enfermedad y su restricción al acceso a la salud por necesidades naturales. En la Tercera parte presento un modelo integral de necesidades y capacidades básicas, comprendiendo la prevención, el tratamiento y el mejoramiento moralmente permisible (genético y no genético.Mi aporte principal consiste en la elaboración de este modelo normativo integral de necesidades y capacidades para la regulación conjunta de la información y terapia genética con los restantes problemas de salud.

  4. Multiplicity and disks within the high-mass core NGC 7538IRS1. Resolving cm line and continuum emission at 0.06″ × 0.05″ resolution

    Science.gov (United States)

    Beuther, H.; Linz, H.; Henning, Th.; Feng, S.; Teague, R.

    2017-09-01

    Context. High-mass stars have a high degree of multiplicity and most likely form via disk accretion processes. The detailed physics of the binary and disk formation are still poorly constrained. Aims: We seek to resolve the central substructures of the prototypical high-mass star-forming region NGC 7538IRS1 at the highest possible spatial resolution in line and continuum emission to investigate the protostellar environment and kinematics. Methods: Using the Karl G. Jansky Very Large Array (VLA) in its most extended configuration at 24 GHz has allowed us to study the NH3 and thermal CH3OH emission and absorption as well as the cm continuum emission at an unprecedented spatial resolution of 0.06″ × 0.05″, corresponding to a linear resolution of 150 AU at a distance of 2.7 kpc. Results: A comparison of these new cm continuum data with previous VLA observations from 23 yr ago reveals no recognizable proper motions. If the emission were caused by a protostellar jet, proper motion signatures should have been easily identified. In combination with the high spectral indices S ∝ να (α between 1 and 2), this allows us to conclude that the continuum emission is from two hypercompact Hii regions separated in projection by about 430 AU. The NH3 spectral line data reveal a common rotating envelope indicating a bound high-mass binary system. In addition to this, the thermal CH3OH data show two separate velocity gradients across the two hypercompact Hii regions. This indicates two disk-like structures within the same rotating circumbinary envelope. Disk and envelope structures are inclined by 33 °, which can be explained by initially varying angular momentum distributions within the natal, turbulent cloud. Conclusions: Studying high-mass star formation at sub-0.1″ resolution allows us to isolate multiple sources as well as to separate circumbinary from disk-like rotating structures. These data show also the limitations in molecular line studies in investigating the

  5. New species of Monepidosis Mamaev, 1966 and Antipodosis gen. nov., a closely related genus from New Zealand (Diptera, Cecidomyiidae

    Directory of Open Access Journals (Sweden)

    Mathias Jaschhof

    2016-04-01

    Full Text Available Three new species of Monepidosis Mamaev, 1966, a Holarctic genus of Porricondylinae (Diptera, Cecidomyiidae, are described: M. heterocera sp. nov. from Sweden and Germany, M. scepteroides sp. nov. from Sweden, and M. shikokuensis sp. nov. from Japan. A new porricondyline genus, Antipodosis gen. nov., is introduced for eight new species from New Zealand, named A. australis gen. et sp. nov., A. elongata gen. et sp. nov., A. granvillensis gen. et sp. nov., A. pureora gen. et sp. nov., A. rakiura gen. et sp. nov., A. rotoiti gen. et sp. nov., A. rotoroa gen. et sp. nov., and A. waipapa gen. et sp. nov. Male genitalic morphology indicates that Monepidosis and Antipodosis gen. nov. are closely related, together forming the Monepidosis group of genera, which stands out from the other Porricondylini. Monepidosis spatulata Spungis, 2006, a species originally described from Latvia and Lithuania, is for the first time reported to occur in Sweden.

  6. Enfermedades de base genética Genetically based diseases

    Directory of Open Access Journals (Sweden)

    D. González-Lamuño

    2008-01-01

    Full Text Available La genética constituye uno de los mayores avances científicos del siglo XX, que comienza con el redescubrimiento de las leyes de Mendel y termina con la elaboración del primer "borrador" de la secuencia completa del genoma humano. La genética utiliza diferentes estrategias de investigación, como los estudios de gemelos y de adopción, que investigan la influencia de los factores genéticos y ambientales, y las estrategias para identificar genes específicos (genética molecular. Además del importante grado de discapacidad que generan, el impacto social de las enfermedades hereditarias es enorme, por su carácter potencialmente recurrente en una misma familia y por el elevado coste socio-sanitario derivado de la enorme carga de cuidados que requiere. El diagnóstico de las enfermedades hereditarias presenta características diferenciadoras muy significativas ya que el resultado de un diagnóstico genético tiene no sólo efectos sobre el paciente sino también sobre todos los individuos emparentados. Por tanto, la unidad de estudio en el diagnóstico genético es la familia y todo proceso de diagnóstico implica una investigación familiar. También conviene tener en cuenta que los protocolos de diagnóstico se desarrollan de forma paralela a la investigación básica y generalmente están poco estandarizados. Los resultados obtenidos en los estudios genéticos y el tipo de información que se facilita al paciente y a su familia deben ser matizados dentro del proceso del "consejo genético".Genetics is one of the greatest scientific advances of the XX century, which begins with the rediscovery of Mendel’s laws and culminates in the elaboration of the first "draft" of the complete sequence of the human genome. Genetics employs different research strategies, such as the study of twins and adoption, investigating the influence of genetic and environmental factors, and strategies for identifying specific genes (molecular genetics. Besides the

  7. GENLAB, Laboratorio Virtual de Genética

    Directory of Open Access Journals (Sweden)

    García S.

    2001-06-01

    Full Text Available GenLab es el nombre que tiene el software diseñado por nosotros en el cual se modela el pro-ceso meiótico y la fecundación en organismos diploides. El objetivo de esta aplicación es el deilustrar el cruce de un resultado determinado, tratando de ser lo más ajustados a la realidad.La modelación de la reproducción sexual se realiza internamente y el GenLab se limita a presen-tar los resultados según el número de la descendencia seleccionado, para un cruce específico,esto significa que se puede escoger una gran cantidad de características para los parentalesy se puede estudiar la frecuencia de estos en la descendencia.

  8. Gen y ética. La inocencia perdida

    OpenAIRE

    Orozco-Martínez, Carlos E.

    1996-01-01

    Después del lanzamiento de las bombas atómicas en agosto de 1945 en Hiroshima y Nagasaki, la ética en la ciencia se convirtió en un tema de interés público con complejas implicaciones sociales. El artículo reflexiona sobre la ética en la investigación genética, vista desde la posibilidad de mejorar la salud de los humanos al predecir o evitar enfermedades, pero también como una puerta hacia la manipulación genética, la discriminación de los débiles y los enfermos, e incluso la alteración del ...

  9. Estructura y diversidad genética en vacas Holstein de Antioquia usando un polimorfismo del gen bGH

    Directory of Open Access Journals (Sweden)

    Juan Rincon F.

    2013-03-01

    Full Text Available Objetivo. Determinar las frecuencias alélicas y genotípicas del polimorfismo del intrón 3 del gen bGH y estimar algunos parámetros de estructura poblacional en ganado Holstein. Materiales y métodos. El estudio se realizó con 1366 vacas Holstein en 120 hatos de 11 municipios del departamento de Antioquia. Se extrajo DNA por el método de Salting out y la genotipificación se realizó usando la técnica de PCR-RFLPs. La diversidad genética se determinó mediante la comparación de las heterocigosidades, El equilibrio de Hardy-Weinberg (HW y la diferenciación genética entre las poblaciones se realizó usando el software Arlequín 2.0 Las frecuencias alélicas y genotípicas se evaluaron mediante el paquete estadístico SAS®. Resultados. Las frecuencias genotípicas encontradas fueron 0.764 (+/+, 0.223 (+/- y 0.013 (-/- y las frecuencias alélicas 0.876 (+ y 0.124 (-. No se encontraron desviaciones del Equilibrio de Hardy Weinberg en ninguna de las subpoblaciones. La diversidad genética determinada mediante la comparación de las heterocigosidades fue relativamente baja entre poblaciones pero al interior de estas no. El valor de FST de toda la población fue de 0.0068 y significativo (p<0.05, algunos FST pareados también lo fueron, tomando valores desde 0.0 a 0.13. Los estadísticos FIT y FIS no fueron significativos. Conclusiones. El gen bGH es un candidato interesante para evaluar características de importancia económica ya que no parece haber sido sometido a selección directa, presenta una variabilidad media en las poblaciones, observándose diferenciación genética significativa entre distintos municipios, producto de los diferentes sistemas de producción y acceso a las biotecnologías.

  10. Dissimilaridade genética entre genótipos de aveia Genetic dissimilarity among oat genotypes

    Directory of Open Access Journals (Sweden)

    Volmir Sergio Marchioro

    2003-04-01

    Full Text Available Dezoito genótipos de aveia foram testados quanto à dissimilaridade genética, com e sem o controle de moléstias da parte aérea. As variáveis avaliadas foram rendimento de grãos desaristados, peso de mil grãos, peso do hectolitro, estatura de planta e dias da emergência à floração. Foram empregados análises por variáveis canônicas e técnicas de agrupamento por meio dos métodos de otimização de Tocher e o método hierárquico do vizinho mais próximo, tendo como medida de dissimilaridade a distância generalizada de Mahalanobis. Pelos resultados, constatou-se significativa dissimilaridade genética entre os genótipos, indicando a existência de variabilidade para os caracteres avaliados. O método de Tocher e o método do vizinho mais próximo permitiram a separação dos genótipos em grupos distintos, possibilitando a identificação de futuros genitores que possam ser utilizados em cruzamentos artificiais que produzam progênies com maior heterose. Os caracteres que mais contribuíram para a dissimilaridade genética foram o peso do hectolitro e a estatura de planta.Eighteen oat genotypes were tested for genetic dissimilarity, with and without shoot disease control. The evaluated variables were grain yield, weight of one thousand grains, test weight, plant stature and days from emergence to flowering. Cannonical variables and clustering methods by Tocher's optimization test and the hierarchical method of nearest neighbor were employed, having as a measure of dissimilarity the general distance of Mahalanobis. The results showed a significant genetic dissimilarity among the genotypes, indicating the presence of genetic variability for the evaluated traits. Tocher's and nearest neighbor methods allowed the separation of genotypes in distinct groups, enabling the identification of potential parents for artificial crosses to obtain progenies with higher heterosis. The major traits contributing to genetic dissimilarity in our study

  11. NextGen Avionics Roadmap, Version 1.2

    Science.gov (United States)

    2010-09-21

    operators at remote locations. Cost and Benefit Considerations Costs to an aircraft operator, whether airline or military come in two forms -- capital... sense of the numbers and types of enablers that may be necessary to support operations that will be in- tegral to NextGen. Historical lead-in times...prediction information. Global Navigation Satellite System ( GNSS ), ADS-B Out, Aircraft Characteristic Database, Aircraft Wake Database, Wake

  12. Applications of Dredging and Beach Fills in GenCade

    Science.gov (United States)

    2016-06-01

    based on the methodology employed in the Inlet Reservoir Model ( IRM ), which assumes that if a jetty is not present at an inlet, all of the sand...system is at equilibrium. Further details about the IRM formulation within GenCade can be found in Frey et al. (2012, 2014). Figure 13 shows the...apportionment of transported sediment it received in the IRM . The only alternative where ERDC/CHL CHETN-IV-109 June 2016 10 the shoal does not

  13. Variabilidad genética en Prosopis ferox (Mimosaceae

    Directory of Open Access Journals (Sweden)

    Alicia D. Burghardt

    2004-01-01

    Full Text Available Prosopis ferox (Mimosaceae es una especie arbustiva o arbórea espinosa que se distribuye desde el Sur de Bolivia hasta el noroeste de la Argentina. En la provincia de Jujuy se encuentra a grandes alturas (entre los 2400 y los 3700 m s.m.. Existe una gran variabilidad morfológica, especialmente en cuanto a las dimensiones del fruto y la cantidad de semillas por fruto, ambas características importantes debido al uso de esta planta como forraje. Con el objeto de verificar si existe además variabilidad genética, se realizó un estudio electroforético de proteínas seminales de árboles procedentes de distintas localidades de la provincia de Jujuy. Los patrones polipeptídicos obtenidos por SDS-PAGE presentaron en total 26 bandas. Cada población se caracterizó por sus patrones de presencia-ausencia de bandas, habiéndose encontrado variabilidad intrapoblacional (polimorfismo en algunas de ellas, siendo otras genéticamente homogéneas. Los índices polimórficos en poblaciones de P. ferox son comparables a los obtenidos previamente en P. ruscifolia. La variabilidad genética interpoblacional hallada por medio del estudio electroforético de las proteínas seminales hace suponer la existencia de ecotipos

  14. The GenABEL Project for statistical genomics.

    Science.gov (United States)

    Karssen, Lennart C; van Duijn, Cornelia M; Aulchenko, Yurii S

    2016-01-01

    Development of free/libre open source software is usually done by a community of people with an interest in the tool. For scientific software, however, this is less often the case. Most scientific software is written by only a few authors, often a student working on a thesis. Once the paper describing the tool has been published, the tool is no longer developed further and is left to its own device. Here we describe the broad, multidisciplinary community we formed around a set of tools for statistical genomics. The GenABEL project for statistical omics actively promotes open interdisciplinary development of statistical methodology and its implementation in efficient and user-friendly software under an open source licence. The software tools developed withing the project collectively make up the GenABEL suite, which currently consists of eleven tools. The open framework of the project actively encourages involvement of the community in all stages, from formulation of methodological ideas to application of software to specific data sets. A web forum is used to channel user questions and discussions, further promoting the use of the GenABEL suite. Developer discussions take place on a dedicated mailing list, and development is further supported by robust development practices including use of public version control, code review and continuous integration. Use of this open science model attracts contributions from users and developers outside the "core team", facilitating agile statistical omics methodology development and fast dissemination.

  15. Crescimento de genótipos de frangos tipo caipira

    Directory of Open Access Journals (Sweden)

    R. C. Veloso

    2015-10-01

    Full Text Available RESUMOObjetivou-se com este trabalho comparar o padrão de crescimento, mediante ajustes das respectivas curvas de crescimento por modelos não lineares, bem como estudar o desenvolvimento de cortes de carcaça em relação ao peso da carcaça em diferentes genótipos de frangos tipo caipira. Foram utilizados 840 pintos de um dia, machos, distribuídos em delineamento inteiramente ao acaso, dos seguintes genótipos da linhagem Redbro: Caboclo, Carijó, Colorpak, Gigante Negro, Pesadão Vermelho, Pescoço Pelado e Tricolor. As aves foram alojadas em 28 boxes, sendo 30 aves/boxe, em galpão de alvenaria com acesso a um piquete de 45m², com quatro repetições. O peso corporal individual dos frangos foi medido ao nascer, aos 14, 28, 42, 56, 70 e 84 dias de idade. Para a determinação das curvas de crescimento do peso corporal das aves, os dados coletados foram avaliados por meio dos modelos não lineares: Brody, Gompertz, Logístico, Richards e von Bertalanffy. Foi empregado o PROC NLIN do SAS, utilizando-se o método interativo de Gauss-Newton. Os critérios usados para escolha do modelo de melhor ajuste da curva de crescimento foram o coeficiente de determinação, o desvio padrão assintótico, o desvio médio absoluto dos resíduos e o índice assintótico. As análises para obtenção dos coeficientes alométricos foram realizadas por meio do PROC GLM do SAS para os genótipos Carijó, Colorpak, Pesadão Vermelho, Pescoço Pelado e Tricolor. Foram avaliados os pesos da carcaça, do peito, das coxas, das sobrecoxas, das pernas e das asas das aves abatidas aos 85 dias de idade. Apenas as equações propostas por Gompertz, von Bertalanffy e Logístico atingiram a convergência, e o modelo proposto por von Bertalanffy foi o mais adequado para descrever o crescimento dos genótipos de frangos caipiras. Todos os cortes avaliados apresentaram crescimento tardio em relação ao peso da carcaça em genótipos de frangos tipo caipira.

  16. From AWE-GEN to AWE-GEN-2d: a high spatial and temporal resolution weather generator

    Science.gov (United States)

    Peleg, Nadav; Fatichi, Simone; Paschalis, Athanasios; Molnar, Peter; Burlando, Paolo

    2016-04-01

    A new weather generator, AWE-GEN-2d (Advanced WEather GENerator for 2-Dimension grid) is developed following the philosophy of combining physical and stochastic approaches to simulate meteorological variables at high spatial and temporal resolution (e.g. 2 km x 2 km and 5 min for precipitation and cloud cover and 100 m x 100 m and 1 h for other variables variable (temperature, solar radiation, vapor pressure, atmospheric pressure and near-surface wind). The model is suitable to investigate the impacts of climate variability, temporal and spatial resolutions of forcing on hydrological, ecological, agricultural and geomorphological impacts studies. Using appropriate parameterization the model can be used in the context of climate change. Here we present the model technical structure of AWE-GEN-2d, which is a substantial evolution of four preceding models (i) the hourly-point scale Advanced WEather GENerator (AWE-GEN) presented by Fatichi et al. (2011, Adv. Water Resour.) (ii) the Space-Time Realizations of Areal Precipitation (STREAP) model introduced by Paschalis et al. (2013, Water Resour. Res.), (iii) the High-Resolution Synoptically conditioned Weather Generator developed by Peleg and Morin (2014, Water Resour. Res.), and (iv) the Wind-field Interpolation by Non Divergent Schemes presented by Burlando et al. (2007, Boundary-Layer Meteorol.). The AWE-GEN-2d is relatively parsimonious in terms of computational demand and allows generating many stochastic realizations of current and projected climates in an efficient way. An example of model application and testing is presented with reference to a case study in the Wallis region, a complex orography terrain in the Swiss Alps.

  17. Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes.

    Science.gov (United States)

    Vieira Vaz, Marcelo Gomes Marçal; Genuário, Diego Bonaldo; Andreote, Ana Paula Dini; Malone, Camila Francieli Silva; Sant'Anna, Célia Leite; Barbiero, Laurent; Fiore, Marli Fátima

    2015-01-01

    The genus Leptolyngbya Anagnostidis & Komárek (1988) was described from a set of strains identified as 'LPP-group B'. The morphology within this group is not particularly informative and underestimates the group's genetic diversity. In the present study, two new pseudanabaenacean genera related to Leptolyngbya morphotypes, Pantanalinema gen. nov. and Alkalinema gen. nov., are described under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants, based on a polyphasic approach. Pantanalinema gen. nov. (type species Pantanalinema rosaneae sp. nov.) has sheaths and trichomes with slight gliding motility, which distinguish this genus from Alkalinema gen. nov. (type species Alkalinema pantanalense sp. nov.), which possesses trichomes arranged in an ornate (interwoven) pattern. 16S rRNA gene sequences of strains of Pantanalinema and Alkalinema exhibited low identity to each other (≤91.6 %) and to other sequences from known pseudanabaenacean genera (≤94.3 and 93.7 %, respectively). In a phylogenetic reconstruction, six sequences from strains of Pantanalinema and four from strains of Alkalinema formed two separate and robust clades (99 % bootstrap value), with the genera Oculatella and Phormidesmis, respectively, as the closest related groups. 16S-23S rRNA intergenic spacer sequences and secondary structures of strains of Pantanalinema and Alkalinema did not correspond to any previous descriptions. The strains of Pantanalinema and Alkalinema were able to survive and produce biomass at a range of pH (pH 4-11) and were also able to alter the culture medium to pH values ranging from pH 8.4 to 9.9. These data indicate that cyanobacterial communities in underexplored environments, such as the Pantanal wetlands, are promising sources of novel taxa.

  18. The Madagascan endemic myrmicine ants related to Eutetramorium (Hymenoptera: Formicidae): taxonomy of the genera Eutetramorium Emery, Malagidris nom. n., Myrmisaraka gen. n., Royidris gen. n., and Vitsika gen. n.

    Science.gov (United States)

    Bolton, Barry; Fisher, Brian L

    2014-04-24

    The monophyletic group of myrmicine ant genera related to Eutetramorium is described and its taxonomy is documented. The group is endemic in Madagascar and contains five genera: Eutetramorium Emery, 1899 (3 species, 1 of which is new); Malagidris nom. n., a replacement name for Brunella Forel, 1917, junior homonym of Brunella Smith, G.W. 1909 (Crustacea) (6 species, 5 of which are new); Myrmisaraka gen. n. (2 species, both new); Royidris gen. n. (15 species, 11 of which are new); Vitsika gen. n. (14 species, all of which are new). Keys to the worker caste are provided for all genera, and provisional keys to known males are given for Malagidris and Vitsika.

  19. A Frequency Diverse Gen2 RFID System with Isolated Continuous Wave Emitters

    Directory of Open Access Journals (Sweden)

    Hsin-Chin Liu

    2007-09-01

    Full Text Available The “Gen2” specification for UHF passive RFID systems released by EPCglobal has become an intense research interest. A Gen2 tag derives its power from the RF wave emitted by a Gen2 RFID reader and responds its modulated backscatter signals to the reader. Due to the large propagation loss, the accessible range of a Gen2 tag is hence limited. Moreover, the readability of a Gen2 tag is often influenced by the multipath fading problem. In order to mitigate the problems, a multi-carrier UHF passive RFID system utilizing the frequency diverse backscatter ability of a Gen2 tag is proposed in a prior work. In this work, a thorough analysis of the system is given. Especially, the appropriate powers of the transceiver and CWEs are derived to make the system more feasible in practical applications.

  20. Targeted Amplicon Sequencing (TAS): A Scalable Next-Gen Approach to Multilocus, Multitaxa Phylogenetics

    OpenAIRE

    2011-01-01

    Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequ...

  1. Arctocypris fuhrmanni, n. gen., n. sp. (Crustacea, Ostracoda, Eucypridinae) from Spitsbergen (Norway).

    Science.gov (United States)

    Petkovski, Trajan K; Scharf, Burkhard; Keyser, Dietmar

    2016-01-14

    Material from Spitsbergen (Norway) collected by Spitzenberger (1996) was reinvestigated. A new genus Arctocypris and a new species Arctocypris. fuhrmanni n. gen. n. sp. are described in the present paper. A key to the genera of the subfamily Eucypridinae is provided. At the moment Arctocypris n. gen. comprises four species: Arctocypris arctica (Olofsson, 1918) comb. nov.; A. dulcifons (Diebel & Pietrzeniuk, 1969) comb. nov.; A. foveata (Delorme, 1968) comb. nov. and Arctocypris fuhrmanni n. gen., n. sp.

  2. Gen 2对智能标签打印技术的影响

    Institute of Scientific and Technical Information of China (English)

    斑马公司

    2008-01-01

    @@ 从Gen 2(Class 1 Generation 2 UHF Air Interface Protocol,第二代UHF空中接口协议)技术规范批准到符合Gen 2标准的标签广泛上市,按ISO、EPCglobal Class 0、第一代(Gen 1)和专有协议制作的数以百万计的RFID标签将要投人使用.

  3. REGENERASI DAN PERBANYAKAN RUMPUT LAUT Kappaphycus alvarezii HASIL TRANSFORMASI GEN SUPEROKSIDA DISMUTASE (MaSOD)

    OpenAIRE

    Emma Suryati; Hidayah Triana; Utut Widiastuti; Andi Tenriulo

    2017-01-01

    Transformasi gen superoxide dismutase (MaSOD) pada rumput laut Kappaphycus alvarezii menggunakan Agrobacterium tumefacient telah dilakukan secara in vitro. Transformasi gen MaSOD ke dalam genom rumput laut diharapkan dapat mengurangi cekaman oksidatif terutama yang disebabkan oleh perubahan suhu, salinitas, dan cemaran logam di perairan. Penelitian ini bertujuan untuk regenerasi rumput laut hasil introduksi gen MaSOD dan non-transgenik pada labu kultur. Regenerasi dan perbanyakan rumput laut ...

  4. Recent changes in the GenBank On-line Service.

    OpenAIRE

    Benton, D

    1990-01-01

    The GenBank On-line Service provides access to the GenBank and EMBL nucleic acid sequence databases and to the Swiss-Prot and GenPept protein sequence databases. Users can query the databases by sequence similarity and annotation keywords and retrieve entries of interest. This access is available through e-mail servers, anonymous FTP, anonymous interactive login, and login to established, password-protected, individual accounts.

  5. Phen-Gen: combining phenotype and genotype to analyze rare disorders.

    Science.gov (United States)

    Javed, Asif; Agrawal, Saloni; Ng, Pauline C

    2014-09-01

    We introduce Phen-Gen, a method that combines patients' disease symptoms and sequencing data with prior domain knowledge to identify the causative genes for rare disorders. Simulations revealed that the causal variant was ranked first in 88% of cases when it was a coding variant-a 52% advantage over a genotype-only approach-and Phen-Gen outperformed other existing prediction methods by 13-58%. If disease etiology was unknown, the causal variant was assigned the top rank in 71% of simulations. Phen-Gen is available at http://phen-gen.org/.

  6. Contribuciones de Sir Roland Fisher a la Estadística Genética

    OpenAIRE

    Jaime Cuadros

    2004-01-01

    Sir Ronald Fisher (18901962) fue profesor de genética y muchas de sus innovaciones estadísticas encontraron expresión en el desarrollo de metodología en estadística genética. Sin embargo, mientras sus contribuciones en estadística matemática son fácilmente identificadas, en genética de poblaciones compartió su supremacía con Sewall Wright (1889 1988) y J. S. S. Haldane (1892 1965). Este documento muestra algunas de las mejores contribuciones de Fisher a las bases de la estadística genética, y...

  7. Seleção de genótipos parentais de acerola com base na divergência genética multivariada

    Directory of Open Access Journals (Sweden)

    CARPENTIERI-PÍPOLO VALÉRIA

    2000-01-01

    Full Text Available Este trabalho teve por objetivo identificar e selecionar genótipos parentais de acerola (Malpighia emarginata L. adequadas a programas de melhoramento genético. Nove caracteres quantitativos de maior importância agronômica foram usados para determinação da distância genética e formação de grupos similares de acessos. O agrupamento pelo método de Tocher, a partir das distâncias generalizadas de Mahalanobis, possibilitou a divisão de 14 genótipos em três grupos. Com base na divergência genética e no caráter agronômico-chave (teor de vitamina C, destacaram-se como mais promissores os cruzamentos dos genótipos: AM Mole pertencente ao grupo III, com os genótipos PR AM, N° 18, PR 17, PR 16, Eclipse, AM 22 e Dominga, todos pertencentes ao grupo I.

  8. Distancias genéticas en poblaciones del NOA

    Directory of Open Access Journals (Sweden)

    Acreche, Noemí

    1996-01-01

    Full Text Available La mayor parte de los trabajos realizados en nuestro país sobre polimorfismos hematológicos, abordan la necesaria descripción de las poblaciones. Se pone de relieve la importancia de encarar estudios, en base a la valiosa información publicada, que vinculen los grupos con técnicas que permitan realizar nuevas inferencias sobre sus relaciones. Conocidas en gran medida en cuanto a sus manifestaciones culturales, pueden aportar desde lo genético a la comprensión de los procesos microevolutivos ocurridos en una región. Para el NOA, se ha considerado la presencia de comunidades aborígenes incluídas en cuatro familias lingüísticas. Se tendrán en cuenta estos complejos como representativos de afinidades que se establecen a partir de estrechas relaciones entre las etnias, no sólo por la lengua, sino también por las características de sus sistemas productivos, religiosidad y organización. En base a las frecuencias génicas publicadas correspondientes a los siguientes alelos: I*A, I*B, I*O; M, N, S, s; Dia , Dib; P1, P2; C, c; D, d, E, e; Le, le; Fya, Fyb; Jka, Jkb; K y k se construyeron tablas de frecuencias. Se estimaron los coeficientes de distancias genéticas que fueron analizados y posteriormente incluídos en la construcción de un fenograma de los grupos de estudio, mediante agrupaciones (Sahn Cluster secuenciales, aglomerativas, jerárquicas y anidadas. De acuerdo a la información recopilada de las frecuencias de los 25 alelos estudiados en trece poblaciones de aborígenes del NOA y Paraguay, las distancias genéticas obtenidas reflejan los caracteres lingüístico-culturales.

  9. Alimentos genéticamente alterados: transgénicos

    OpenAIRE

    García González, Jaime

    2016-01-01

    Se definen los términos relacionados directamente con la temática del artículo para luego citar algunas de las contradicciones de los promotores de este tipo de alimentos. Posteriormente, se mencionan los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos genéticamente alterados. Además, se destacan las principales incertidumbres y cuestionamientos señalados y reconocidos por parte de diversos autores y organizaciones de profesionales en la salud, entre ellas...

  10. Cleaning the GenBank Arabidopsis thaliana data set

    DEFF Research Database (Denmark)

    Korning, Peter G.; Hebsgaard, Stefan M.; Rouze, Pierre;

    1996-01-01

    extracted a data set from the A. thaliana entries in GenBank. A number of simple `sanity' checks, based on the nature of the data, revealed an alarmingly high error rate. More than 15% of the most important entries extracted did contain erroneous information. In addition, a number of entries had directly...... common. It is proposed that the level of error correction should be increased and that gene structure sanity checks should be incorporated - also at the submitter level - to avoid or reduce the problem in the future. A non-redundant and error corrected subset of the data for A. thaliana is made available...

  11. Estudio de polimorfismos genéticos en tiroiditis autoinmune

    OpenAIRE

    Iglesias López, Rosa Ana

    2015-01-01

    [ES]Estudio epidemiológico caso-control para comprobar la hipótesis de que ciertos polimorfismos genéticos relacionados con las citosinas y con el proceso de autofagia pueden modificar el riesgo de presentar tiroiditis autoinmune. Se realiza el estudio sobre 200 pacientes con tiroiditis autoinmune reclutados en el Servicio de Endocrinología y Nutrición en el Complejo Asistencial Universitario de Salamanca, en los que se confirma la ausencia de diferencias en cuanto a características epidemiol...

  12. Estudio de polimorfismos genéticos en tiroiditis autoinmune

    OpenAIRE

    Iglesias López, Rosa Ana

    2015-01-01

    [ES]Estudio epidemiológico caso-control para comprobar la hipótesis de que ciertos polimorfismos genéticos relacionados con las citosinas y con el proceso de autofagia pueden modificar el riesgo de presentar tiroiditis autoinmune. Se realiza el estudio sobre 200 pacientes con tiroiditis autoinmune reclutados en el Servicio de Endocrinología y Nutrición en el Complejo Asistencial Universitario de Salamanca, en los que se confirma la ausencia de diferencias en cuanto a características epidemiol...

  13. Epistemología genética.

    OpenAIRE

    Gutiérrez Vásquez, Ramiro; Facultad de Psicología, Universidad Peruana Cayetano Heredia.

    2015-01-01

    Para recrear las ideas de Jean Piaget sobre su epistemología genética se ha visto por conveniente abordar el trabajo desde varios aspectos: abordar el problema de su significado y de lo que estudia; fundamentar su estatus de ciencia, característica central que la diferencia de otras posturas epistemológicas; probar que la psicología juega un rol importante al momento de analizar la ciencia. Del mismo modo, se estudia los métodos y procedimientos originales con los que Piaget abordó los temas ...

  14. Genética do autismo Genetics of autism

    OpenAIRE

    Gianna Carvalheira; Naja Vergani; Décio Brunoni

    2004-01-01

    O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.Autism is a neuropsychiatric disorder with profound family and social consequences. An extraordinary number of genetical-clinical, cytogenetics and molecular studies we...

  15. Genética de la diabetes mellitus tipo 2

    OpenAIRE

    Carmen Carrillo; Arturo Panduro Cerda

    2001-01-01

    Factores genéticos predisponen a la diabetesmellitus (DM) tipo 2 y el desarrollo de la enfermedad depende en gran parte de la alimentación y actividad física (factores ambientales).Existen familias cuyos miembros presentan DMtipo 2 solamente o bien diferentes tipos de diabetes. En general se presenta un patrón de herencia multifactorial, rara vez autosómico dominante o mitocondrial. El riesgo que tienen losfamiliares de pacientes con DM tipo 2 se establece con el valor lambda el cual depende ...

  16. TrayGen: Arranging objects for exhibition and packaging

    KAUST Repository

    Yang, Yongliang

    2013-10-01

    We present a framework, called TrayGen, to generate tray designs for the exhibition and packaging of a collection of objects. Based on principles from shape perception and visual merchandising, we abstract a number of design guidelines on how to organize the objects on the tray for the exhibition of their individual features and mutual relationships. Our framework realizes these guidelines by analyzing geometric shapes of the objects and optimizing their arrangement. We demonstrate that the resultant tray designs not only save space, but also highlight the characteristic of each object and the inter-relations between objects. © 2013 The Eurographics Association and John Wiley & Sons Ltd.

  17. Enfermedades genéticas más frecuentes en pacientes atendidos en consulta de genética clínica

    OpenAIRE

    Elibett Carcasés Carcasés; Nora María Orive Rodríguez; Lisset del Carmen Romero Portelles; Glenys Katiuska Silva González

    2015-01-01

    La estimación de la prevalencia de las enfermedades genéticas se dificulta, entre otras causas, por su rareza. Se realizó un estudio descriptivo retrospectivo, para identificar las enfermedades genéticas de mayor prevalencia en pacientes atendidos por este programa en el Centro Provincial de Genética Médica de Las Tunas, Cuba; desde el año 1989 hasta julio de 2014. Se revisaron todas las historias clínicas. Predominó el origen monogénico (69 %), siendo los síndromes dismórficos los más numero...

  18. Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus.

    Science.gov (United States)

    Busse, Hans-Jürgen

    2016-01-01

    In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and polar lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, polar lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and polar lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species).

  19. Moureanthidium, gen.n. de Dianthidiini do Brasil (Hymenoptera, Megachilidae Moureanthidium, gen.n. de Dianthidiini from Brazil (Hymenoptera, Megachiliiae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    1995-01-01

    Full Text Available Moureanthidium, gen.n. is proposed for Dianthidium subarenarium Schwarz, 1933 and to include four new species of Anthidiine bees from Brazil, Moureanthidium paranaense, sp.n from Ponta Grossa, Paraná, Moureanthidium capixaba, sp.n. from Santa Teresa, Espírito Santo, Moureanthidium bahianum, sp.n. from Vitória da Conquista, Bahia and Moureanthidium catarinense, sp.n. from Seara, Santa Catarina. The males are characterized by the border of the seventh tergum strongly reflexed, densely hairy and with a pair of lateral projections and the third sternite with two spines or little projections at the middle, near the apex.

  20. Genética y genómica enfocadas en el estudio de la resistencia bacteriana

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  1. GenGIS: A geospatial information system for genomic data

    Science.gov (United States)

    Parks, Donovan H.; Porter, Michael; Churcher, Sylvia; Wang, Suwen; Blouin, Christian; Whalley, Jacqueline; Brooks, Stephen; Beiko, Robert G.

    2009-01-01

    The increasing availability of genetic sequence data associated with explicit geographic and ecological information is offering new opportunities to study the processes that shape biodiversity. The generation and testing of hypotheses using these data sets requires effective tools for mathematical and visual analysis that can integrate digital maps, ecological data, and large genetic, genomic, or metagenomic data sets. GenGIS is a free and open-source software package that supports the integration of digital map data with genetic sequences and environmental information from multiple sample sites. Essential bioinformatic and statistical tools are integrated into the software, allowing the user a wide range of analysis options for their sequence data. Data visualizations are combined with the cartographic display to yield a clear view of the relationship between geography and genomic diversity, with a particular focus on the hierarchical clustering of sites based on their similarity or phylogenetic proximity. Here we outline the features of GenGIS and demonstrate its application to georeferenced microbial metagenomic, HIV-1, and human mitochondrial DNA data sets. PMID:19635847

  2. Next gen wavelets down-sampling preserving statistics

    Science.gov (United States)

    Szu, Harold; Miao, Lidan; Chanyagon, Pornchai; Cader, Masud

    2007-04-01

    We extend the 2 nd Gen Discrete Wavelet Transform (DWT) of Swelden to the Next Generations (NG) Digital Wavelet Transform (DWT) preserving the statistical salient features. The lossless NG DWT accomplishes the data compression of "wellness baseline profiles (WBP)" of aging population at homes. For medical monitoring system at home fronts we translate the military experience to dual usage of veterans & civilian alike with the following three requirements: (i) Data Compression: The necessary down sampling reduces the immense amount of data of individual WBP from hours to days and to weeks for primary caretakers in terms of moments, e.g. mean value, variance, etc., without the artifacts caused by FFT arbitrary windowing. (ii) Lossless: our new NG_DWT must preserve the original data sets. (iii) Phase Transition: NG_DWT must capture the critical phase transition of the wellness toward the sickness with simultaneous display of local statistical moments. According to the Nyquist sampling theory, assuming a band-limited wellness physiology, we must sample the WBP at least twice per day since it is changing diurnally and seasonally. Since NG_DWT, like the 2 nd Gen, is lossless, we can reconstruct the original time series for the physicians' second looks. This technique of NG_DWT can also help stock market day-traders monitoring the volatility of multiple portfolios without artificial horizon artifacts.

  3. Enfermedades genéticas del ADN mitocondrial humano

    Directory of Open Access Journals (Sweden)

    Solano Abelardo

    2001-01-01

    Full Text Available Las enfermedades mitocondriales son un grupo de trastornos que están producidos por un fallo en el sistema de fosforilación oxidativa (sistema Oxphos, la ruta final del metabolismo energético mitocondrial, con la consiguiente deficiencia en la biosíntesis del trifosfato de adenosina (ATP, por sus siglas en inglés. Parte de los polipéptidos que componen este sistema están codificados en el ácido desoxirribonucleico (DNA mitocondrial y, en los últimos años, se han descrito mutaciones que se han asociado con síndromes clínicos bien definidos. Las características genéticas del DNA mitocondrial, herencia materna, poliplasmia y segregación mitótica, confieren a estas enfermedades propiedades muy particulares. Las manifestaciones clínicas de estas enfermedades son muy heterogéneas y afectan a distintos órganos y tejidos por lo que su correcto diagnóstico implica la obtención de datos clínicos, morfológicos, bioquímicos y genéticos. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html

  4. Interação genótipo x ambiente em genótipos de feijão-caupi semiprostrado via modelos mistos

    Directory of Open Access Journals (Sweden)

    Francisco Eduardo Torres

    2015-01-01

    Full Text Available A metodologia de modelos mistos (REML/BLUP tem sido empregada para estudar os efeitos da interação genótipo x ambiente (G x E em várias culturas, como: arroz, feijão, cana-de-açúcar, cajueiro e eucalipto, porém ainda não foi aplicada em feijão-caupi. Assim, o objetivo deste trabalho foi selecionar simultaneamente genótipos de feijão-caupi semiprostrado cultivados no Estado do Mato Grosso do Sul, via modelos mistos, que reúnam alta adaptabilidade, estabilidade e produtividade de grãos. Foram conduzidos quatro ensaios de valor de cultivo e uso com genótipos de genótipos de feijão-caupi nos anos de 2005 e 2006 em Aquidauana, Chapadão do Sul e Dourados. O delineamento experimental utilizado foi o de blocos completos casualizados, com 20 genótipos e 4 repetições. Os parâmetros genéticos foram estimados pela metodologia REML/BLUP, e a seleção baseou-se no método da média harmônica do desempenho relativo dos valores genéticos (MHPRVG, em três estratégias: seleção com base no valor genético predito, tendo-se considerado o desempenho médio dos genótipos em todos os ambientes (sem efeito de interação ou o desempenho em cada ambiente (com efeito da interação; e seleção simultânea quanto à produtividade de grãos, estabilidade e adaptabilidade. Os genótipos BRS Paraguaçu, MNC99-542F-5 e MNC99-508G-1 podem ser cultivados em vários ambientes, pois reúnem alta produtividade de grãos, adaptabilidade e estabilidade. A herdabilidade da média dos genótipos apresentou magnitude variando de moderada a alta, fato que indica excelentes possibilidades para a seleção, permitindo acurácia seletiva de 82%.

  5. Update History of This Database - GenLibi | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us GenLibi Up...date History of This Database Date Update contents 2014/03/25 GenLibi English archive site is open...base Database Description Download License Update History of This Database Site Policy | Contact Us Up

  6. How is the Xpert MRSA Gen 3 assay (Cepheid) performing on pooled eSwab medium?

    Science.gov (United States)

    Jonckheere, Stijn; Van Vaerenbergh, Kristien; Boel, An; Vankeerberghen, Anne; De Beenhouwer, Hans

    2015-11-01

    The performance of the Xpert MRSA Gen 3 was compared to the Xpert MRSA on pooled eSwab media from nose, throat, and perineum using broth enriched cultured as gold standard. A lower specificity was found for the Xpert MRSA Gen 3 compared to the Xpert MRSA (91.8% versus 97.9%; P<0.05).

  7. Pseudolimia n. gen., a new monotypic genus for Limia heterandria Regan, 1913 (Teleostei: Poeciliidae)

    NARCIS (Netherlands)

    Poeser, Fred N.

    2002-01-01

    A new genus, Pseudolimia n. gen. is established for the South American Limia heterandria Regan, 1913. It is compared in an anatomical analysis with several nominal taxa of the tribe Poeciliini. Based on number and shape of the gonapophyses, Pseudolimia n. gen. is assigned to the tribe Poeciliini.

  8. A 48-plex autosomal SNP GenPlex™ assay for human individualization and relationship testing

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Børsting, Claus; Morling, Niels

    2012-01-01

    SNPs are being increasingly used by forensic laboratories. Different platforms have been developed for SNP typing. We describe the GenPlex™ HID system protocol, a new SNP-typing platform developed by Applied Biosystems where 48 of the 52 SNPforID SNPs and amelogenin are included. The GenPlex™ HID...

  9. 77 FR 54648 - Seventh Meeting: RTCA NextGen Advisory Committee (NAC)

    Science.gov (United States)

    2012-09-05

    ... Passport Country of Citizenship Birthdate and Place of Birth (city and country) Passport and Visa Numbers... Implementing Metroplex capabilities Environmental Issues Impacting NextGen Implementation A background briefing by FAA Environmental Office on the Environmental review process for NextGen capabilities and...

  10. ANALISIS SEKUEN GEN GLUTATION PEROKSIDASE (GPX1 SEBAGAI DETEKSI STRES OKSIDATIF AKIBAT INFEKSI MYCOBACTERIUM TUBERCULOSIS

    Directory of Open Access Journals (Sweden)

    Ari Yuniastuti

    2013-02-01

    Full Text Available Glutation merupakan antioksidan yang berperan dalam fungsi imun, dan diekspresikan secara genetik oleh urutan gen yang membentuk protein enzim Glutation Peroxidase (GPx1. Bila ekspresi gen berubah maka terjadi perubahan fungsi glutation dan kerentanan terhadap stress oksidatif. Metode yang digunakan adalah Kasus-kontrol. Sampel yang digunakan adalah sampel darah. Kelompok kasus adalah sampel darah pasien tuberkulosis paru sedangkan kelompok kontrol adalah sampel darah orang sehat. Pemeriksaan gen Glutation peroxidase (GPx1 menggunakan metode Polymerase Chain Reaction (PCR untuk melihat pita DNA pada pasien tuberkulosis par serta elektroforesis produk PCR-RFLP gen GPx1 kelompok sampel tuberkulosis. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang bermakna antara polimorfisme gen GPx1 (p=0,365 pasein tuberkulois dengan individu sehat, sehingga tidak dapat digunakan sebagai alat deteksi kerentanan terhadap stress oksidatif pada pasien tuberkulosis. Perlu penelitian lanjutan yang menggunakan sampel lebih besar dan populasi etnik yang berbeda.

  11. Polimorfisme Gen Apolipoprotein E Pada Penderita Sindrom Down Trisomi 21

    Directory of Open Access Journals (Sweden)

    Malinda Meinapuri

    2013-01-01

    Full Text Available AbstrakLatar Belakang: Sindrom Down merupakan suatu kelainan kromosom yang ditandai dengan adanya baik seluruhnya (trisomi 21 maupun sebagian (translokasi suatu salinan tambahan kromosom ke 21. Apolipoprotein E (APOE merupakan suatu bentuk protein polimorfik yang disandikan oleh suatu gen yang berlokasi pada lengan panjang kromosom 19 pada posisi 13.2 (19q13.2. Polimorfism gen APOE berkaitan dengan meningkatnya frekuensi alel ε4 yang berakibat terjadinya hambatan dalam percabangan dan pertumbuhan neuron. Dimungkinkan, penderita Sindrom Down Trisomi 21 memiliki gen APOE yang berbeda dengan kontrol sebagai faktor yang dapat mengakibatkan penuaan dini otak. Metode : Penelitian ini merupakan penelitian kasus kontrol untuk mengamati perbedaan distribusi dan frekuensi alel dan genotip gen APOE pada penderita Sindrom Down trisomi 21 dibandingkan dengan kontrol. Kasus Sindrom Down dan kontrol diambil dari data sekunder yang tersimpan di Center for Biomedical Research (CEBIOR Semarang Indonesia. Ekstraksi DNA dilakukan dengan menggunakan metode yang terdapat di (CEBIOR Semarang Indonesia. Kegiatan selanjutnya adalah pemeriksaan polimorfisme gen Apolipoprotein E dengan mengunakan teknik PCR dan RFLP. Hasil : Sebanyak 33 sampel dari penderita Sindrom Down, 18 laki-laki dan 15 perempuan dan 33 sampel kontrol, 18 laki-laki dan 15 perempuan. Baik sampel Sindrom Down maupun kontrol memiliki frekuensi alel ε3 paling tinggi dibandingkan dengan alel ε2 dan ε4. Pada Sindrom Down didapatkan alel ε4 4 sampel (6,1% dan alel ε2 8 sampel (12,1%. Baik sampel Sindrom Down maupun kontrol memiliki genotip ε3/ε3 paling tinggi dibandingkan dengan genotip gen APOE lainnya. Pada Sindrom Down didapatkan genotip ε2/ε4 4 sampel (12,1% dan genotip ε2/ε2 2 sampel (6,1%. Simpulan : Terdapat perbedaan distribusi alel dan genotip gen APOE pada penderita Sindrom Down trisomi 21 dibandingkan dengan kelompok kontrol. Diperlukan analisis sampel yang lebih banyak untuk

  12. Genética da resistência à ferrugem-da-folha em aveia

    Directory of Open Access Journals (Sweden)

    Cruz Renata Pereira da

    2001-01-01

    Full Text Available A ferrugem-da-folha (Puccinia coronata f. sp. avenae é a principal doença da cultura da aveia (Avena sativa L., e o uso de cultivares resistentes é o método de controle mais importante. Este trabalho teve por objetivo determinar o controle genético da resistência à ferrugem-da-folha em aveia e identificar fontes de genes diferentes para resistência a esta doença. Foram utilizados três genótipos resistentes (UFRGS 15, UFRGS 881920 e UFRGS 86A1194-2, três genótipos suscetíveis (UFRGS 7, UFRGS 8 e UFRGS 14 e a geração segregante F3 proveniente dos cruzamentos entre estes genótipos. As plantas foram avaliadas individualmente quanto à presença ou ausência da ferrugem-da-folha, sendo os dados destas leituras utilizados numa análise genética em que a hipótese de um ou dois genes de resistência foi testada pelo qui-quadrado. Os resultados evidenciaram um gene dominante de resistência no genótipo UFRGS 881920 e dois genes complementares no genótipo UFRGS 15 quando estes foram cruzados com os suscetíveis. A análise genética feita não permitiu determinar se estes dois genótipos são ou não a mesma fonte genética de resistência.

  13. Reflexo da interação genótipo x ambiente sobre o melhoramento genético de feijão

    Directory of Open Access Journals (Sweden)

    Thayse Cristine Vieira Pereira

    2015-01-01

    Full Text Available RESUMO: O objetivo foi avaliar os componentes da variância fenotípica e estimar a influência da interação genótipo*ambiente no rendimento de grãos em feijão. Os componentes da variância fenotípica foram estimados pelo método da máxima verossimilhança restrita e do melhor preditor linear não viesado (REML/BLUP, juntamente com o espaço de inferência específico. As avaliações foram realizadas nas safras agrícolas de 2006/07 a 2011/12 no município de Lages/SC. Durante o período, 104 genótipos foram avaliados. Os dados são desbalanceados, sendo que 13 genótipos permaneceram nos ensaios em todos os anos. Observando os resultados, foi possível visualizar que a grande variação (59,0% no comportamento dos genótipos ao longo dos anos é atribuída principalmente à variância do ambiente (σ2a=436.245. Houve diferença significativa entre genótipos para todos os ambientes. Porém, a diferença entre eles foi constante, ou seja, os genótipos não responderam de modo diferenciado frente aos ambientes. A interação genótipo*ambiente (σ2ga=1.368 responde preponderantemente por uma ínfima alteração (0,2% na variação fenotípica, não discriminando de genótipos de feijão quanto ao rendimento de grãos em Lages/SC. Este fato favorece programas de melhoramento vegetal, onde a interação genótipo*ambiente poderia dificultar a distinção, seleção e recomendação de constituições genotípicas superiores. Nessa situação, processos de recomendação de cultivares (ensaio de valor de cultivo e uso - VCU que mantenham os mesmos genótipos dispensam avaliações sucessivas, pois o ranqueamento é paralelo no decorrer dos anos.

  14. GenASiS: General Astrophysical Simulation System. I. Fundamentals

    CERN Document Server

    Cardall, Christian Y; Endeve, Eirik; Mezzacappa, Anthony

    2012-01-01

    GenASiS (General Astrophysical Simulation System) is a new code being developed initially and primarily, though by no means exclusively, for the simulation of core-collapse supernovae on the world's leading capability supercomputers. Using the features of Fortran 2003 that allow for object-oriented programming, its classes are grouped into three major divisions: (1) Basics, which contains some basic utilitarian functionality for large-scale simulations on distributed-memory supercomputers; (2) Mathematics, which includes generic mathematical constructs and solvers that are as agnostic as possible with regard to the specifics of any particular system; and (3) Physics, which sets up physical spaces associated with various theories of spacetime (including gravity), defines various forms of stress-energy, and combines these into `universes.' To provide a foundation for subsequent papers focusing on the implementation of various pieces of physics needed for the simulation of core-collapse supernovae and other astr...

  15. International Conference on NextGen Electronic Technologies

    CERN Document Server

    Thalmann, Nadia; Bhaaskaran, V

    2017-01-01

    This book is a collection of keynote lectures from international experts presented at International Conference on NextGen Electronic Technologies (ICNETS2-2016). ICNETS2 encompasses six symposia covering all aspects of electronics and communications domains, including relevant nano/micro materials and devices . This volume comprises of recent research in areas like computational signal processing analysis, intelligent embedded systems, nanoelectronic materials and devices, optical and microwave technologies, VLSI design: circuits systems and application, and wireless communication networks, and the internet of things. The contents of this book will be useful to researchers, professionals, and students working in the core areas of electronics and their applications, especially to signal processing, embedded systems, and networking.

  16. What Can a Historian Do with AstroGen?

    Science.gov (United States)

    Tenn, Joseph S.

    2015-01-01

    "Astrogen", the Astronomy Genealogy Project, is in the development stage. Patterned after the Mathematics Genealogy Project at http://genealogy.math.ndsu.nodak.edu, it will eventually include most of the world's astronomers, past and present, and provide information about their years of life, highest degrees, universities, and thesis titles. There will also be links to online theses, home pages, and obituaries when these are available. Although a few details remain to be worked out before it becomes public, it is possible to make some use of what has already been compiled. I will give an example, comparing graduates of Harvard University, the University of California at Berkeley, and the University of Chicago from different decades, with information about their professional careers and publication records. The author welcomes queries about AstroGen and is seeking more participants.

  17. O sujeito na epistemologia genética

    OpenAIRE

    Abib,José Antônio Damásio

    2003-01-01

    Sugere-se neste ensaio que, na epistemologia genética, o sujeito se dirige ao mundo, se interessa por ele e é um princípio de auto-organização. Ontologicamente, seu modo de existência é o do encontro: encontro com o mundo. Epistemologicamente, seu modo de conhecimento é o da presença: presença do mundo. O construtivismo, a tese de que o conhecimento é construção de relações, começa como presença, o que exclui a noção de conhecimento como representação. Destaca-se a centralidade da noção de au...

  18. How Gen Y and Boomers will reshape your agenda.

    Science.gov (United States)

    Hewlett, Sylvia Ann; Sherbin, Laura; Sumberg, Karen

    2009-01-01

    When it comes to workplace preferences, Generation Y workers closely resemble Baby Boomers. Because these two huge cohorts now coexist in the workforce, their shared values will hold sway in the companies that hire them. The authors, from the Center for Work-Life Policy, conducted two large-scale surveys that reveal those values. Gen Ys and Boomers are eager to contribute to positive social change, and they seek out workplaces where they can do that. They expect flexibility and the option to work remotely, but they also want to connect deeply with colleagues. They believe in employer loyalty but desire to embark on learning odysseys. Innovative firms are responding by crafting reward packages that benefit both generations of workers--and their employers.

  19. Commissioning and Performance Analysis of WhisperGen Stirling Engine

    Science.gov (United States)

    Pradip, Prashant Kaliram

    Stirling engine based cogeneration systems have potential to reduce energy consumption and greenhouse gas emission, due to their high cogeneration efficiency and emission control due to steady external combustion. To date, most studies on this unit have focused on performance based on both experimentation and computer models, and lack experimental data for diversified operating ranges. This thesis starts with the commissioning of a WhisperGen Stirling engine with components and instrumentation to evaluate power and thermal performance of the system. Next, a parametric study on primary engine variables, including air, diesel, and coolant flowrate and temperature were carried out to further understand their effect on engine power and efficiency. Then, this trend was validated with the thermodynamic model developed for the energy analysis of a Stirling cycle. Finally, the energy balance of the Stirling engine was compared without and with heat recovery from the engine block and the combustion chamber exhaust.

  20. Festival du rire de Genève

    CERN Multimedia

    Staff Association

    2015-01-01

    Connaissez-vous le Festival du rire de Genève ? La deuxième édition aura lieu du 25 au 28 mars 2015 au Casino-Théâtre à Carouge. Côté programmation, Marc Donnet-Monay ouvre les festivités avant trois autres soirées de folie et d’humour que nous vous laissons le soin de découvrir dans le programme : http://www.rire-geneve.ch/#programme. Réduction de 30% sur l’achat de places pour les membres du personnel du CERN. Pour cela, il suffit de se rendre sur la billetterie en ligne de notre site : www.rire-geneve.ch et d’utiliser le code promotionnel. Contacter le secrétariat de l’Association du personnel (Staff.Association@cern.ch) pour connaitre ce code promotionnel.

  1. Genética de las epilepsias Genetics of epilepsy

    Directory of Open Access Journals (Sweden)

    Gustavo A. Charria-Ortiz

    2007-01-01

    Full Text Available En años recientes se ha podido definir con gran exactitud la existencia de alteraciones genéticas específicas en una gran variedad de síndromes epilépticos tradicionales. Es decir, por vez primera se ha podido relacionar de manera contundente y predecible la presencia de alteraciones genómicas y/o proteómicas con síndromes epilépticos antes considerados como "idiopáticos". La gran mayoría de dichos defectos han sido encontrados en genes codificadores para canales iónicos y/o receptores de membrana, lo cual en cierto modo confirma la ya antes postulada relevancia que estas estructuras tienen en la actividad electroquímica espontánea neuronal cuyo desajuste conllevaría a ciertas formas de epilepsia. Esta revisión se centra en los aspectos genéticos y clínicos de dichas condiciones y alteraciones. También se revisarán brevemente los estudios más relevantes de la literatura médica según los cuales -aun a pesar de no haberse definido con la misma exactitud el tipo de anomalías etiológicas- puede tranquilamente inferirse el gran componente genético que parece subyacer a la etiología de las epilepsias. Por ultimo se enfatizará en que a pesar de dichos descubrimientos, su aplicación en la práctica clínica diaria aun es muy limitada, no solo por la relativa rareza de algunos de tales síndromes neurológicos sino también por la poca relevancia que hasta ahora ellos han tenido en el manejo médico rutinario de la mayoría de los pacientes. Las posibilidades inmediatas de tales avances -incluida la farmacogenómica-, así como los posibles conflictos éticos en que se podría incurrir serán también brevemente discutidos.In the last few years, the presence of specific genetic abnormalities leading to some of the classical epileptic syndromes has been clearly elucidated. This means that for the first time, it has become possible to create a strong relationship between the presence of specific genomic and/or proteomic

  2. Genética do autismo Genetics of autism

    Directory of Open Access Journals (Sweden)

    Gianna Carvalheira

    2004-12-01

    Full Text Available O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.Autism is a neuropsychiatric disorder with profound family and social consequences. An extraordinary number of genetical-clinical, cytogenetics and molecular studies were done in recent years. A multiloci epistatic model involved in the causation of autism have emerged from these studies.

  3. Structure and reactivity of a pyridine-1-imido-2-thiolato complex of iridium(III), CpIr(1-N-2-Spy), generated by photolysis of the (azido)(pyridine-2-thiolato) complex, CpIr(2-Spy)(N3).

    Science.gov (United States)

    Sekioka, Yusuke; Kaizaki, Sumio; Mayer, James M; Suzuki, Takayoshi

    2005-11-14

    Photolysis of the (azido)(pyridine-2-thiolato)iridium(III) complex CpIr(2-Spy)(N3) (1) gave a pyridine-1-imido-2-thiolato complex, CpIr(1-N-2-Spy) (2), in which one of the nitrogen atoms of the azide ligand has been inserted into the Ir-N(py) bond (Cp = eta5-C5Me5). Complex 2 reacted quantitatively with methyl iodide to give the N-methylated product, [CpIr(1-NMe-2-Spy)]I (3). X-ray crystallography revealed that both 2 and 3 have similar two-legged piano stool structures with planar 1-N-2-Spy2- or 1-NMe-2-Spy- ligands, which form iridacyclopentadienyl-like rings by moderate S(ppi)/N(ppi) to Ir(dpi) pi donation.

  4. New Boletaceae taxa from Guyana: Binderoboletus segoi gen. and sp. nov., Guyanaporus albipodus gen. and sp. nov., Singerocomus rubriflavus gen. and sp. nov., and a new combination for Xerocomus inundabilis.

    Science.gov (United States)

    Henkel, Terry W; Obase, Keisuke; Husbands, Dillon; Uehling, Jessie K; Bonito, Gregory; Aime, M Catherine; Smith, Matthew E

    2016-01-01

    Binderoboletus segoi gen. and sp. nov., Guyanaporus albipodus gen. and sp. nov. and Singerocomus rubriflavus gen. and sp. nov. (Boletaceae, Boletales, Basidiomycota) are described from the Pakaraima Mountains and adjacent lowlands of Guyana. Xerocomus inundabilis, originally described from the central Brazilian Amazon and based solely on the type collection, is redescribed from numerous collections from Guyana and transferred into Singerocomus. These boletes occur in Neotropical forests dominated by ectomycorrhizal trees in the genera Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae) and Pakaraimaea (Dipterocarpaceae). Three of the species were repeatedly found in a multiyear sporocarp survey in Dicymbe corymbosa-monodominant forest. Macromorphological, micromorphological, habitat and multilocus DNA sequence data are provided for each species. A molecular phylogenetic analysis based on a large taxon set across the Boletaceae justifies erection of the new genera.

  5. Hand-held optical imager (Gen-2): improved instrumentation and target detectability.

    Science.gov (United States)

    Gonzalez, Jean; Decerce, Joseph; Erickson, Sarah J; Martinez, Sergio L; Nunez, Annie; Roman, Manuela; Traub, Barbara; Flores, Cecilia A; Roberts, Seigbeh M; Hernandez, Estrella; Aguirre, Wenceslao; Kiszonas, Richard; Godavarty, Anuradha

    2012-08-01

    Hand-held optical imagers are developed by various researchers towards reflectance-based spectroscopic imaging of breast cancer. Recently, a Gen-1 handheld optical imager was developed with capabilities to perform two-dimensional (2-D) spectroscopic as well as three-dimensional (3-D) tomographic imaging studies. However, the imager was bulky with poor surface contact (~30%) along curved tissues, and limited sensitivity to detect targets consistently. Herein, a Gen-2 hand-held optical imager that overcame the above limitations of the Gen-1 imager has been developed and the instrumentation described. The Gen-2 hand-held imager is less bulky, portable, and has improved surface contact (~86%) on curved tissues. Additionally, the forked probe head design is capable of simultaneous bilateral reflectance imaging of both breast tissues, and also transillumination imaging of a single breast tissue. Experimental studies were performed on tissue phantoms to demonstrate the improved sensitivity in detecting targets using the Gen-2 imager. The improved instrumentation of the Gen-2 imager allowed detection of targets independent of their location with respect to the illumination points, unlike in Gen-1 imager. The developed imager has potential for future clinical breast imaging with enhanced sensitivity, via both reflectance and transillumination imaging.

  6. Factors genètics de la leishmaniosi canina: caracterització del Nramp1 com a gen candidat

    OpenAIRE

    Altet i Sanahujes, Laura

    2002-01-01

    Descripció del recurs: el 22 d'octubre de 2003 L'objectiu d'aquesta tesi és la recerca de gens candidats de resistència o susceptibilitat a leishmaniosi canina. Aquesta malaltia, que és endèmica en l'area mediterranea, és una zoonosi causada pel paràsit Leishmania infantum, on el gos és el principal reservori. A més, està guanyant molta importància en l'home, ja que ha esdevingut una de les coinfeccions principals en malalts del virus de la SIDA. El món dels gossos aporta un material molt ...

  7. Avaliação genética para peso corporal em um rebanho Nelore

    Directory of Open Access Journals (Sweden)

    F.M Gonçalves

    2011-02-01

    Full Text Available Estimaram-se as herdabilidades para os efeitos genéticos direto e materno e as correlações genéticas entre essas variáveis para os pesos ao desmame (P205, ao ano (P365 e ao sobreano (P550 em um rebanho Nelore do norte de Minas Gerais. O modelo estatístico incluiu os efeitos aditivos direto e materno, os efeitos fixos de grupo de contemporâneos (fazenda, sexo, regime alimentar, estação (seca e água e ano de nascimento do animal e o efeito da covariável idade da vaca ao parto (linear e quadrático. Os componentes de variância e os valores genéticos foram estimados utilizando-se o método REML. A tendência genética foi obtida utilizando-se a regressão do valor genético médio anual em relação ao ano de nascimento dos animais. As estimativas de herdabilidade do efeito aditivo direto ( para P205, P365 e P550 foram 0,60, 0,69 e 0,75, respectivamente. Estes coeficientes de são de alta magnitude, indicando que o rebanho apresenta variabilidade genética aditiva relativa e, portanto, espera-se progresso genético considerável utilizando a seleção. Pela análise da tendência genética, verificou-se que houve evolução nos valores genéticos dos animais ao longo dos anos estudados.

  8. Estructura genética poblacional del gen lactoferrina bovino en vacas Holstein del departamento de Antioquia

    Directory of Open Access Journals (Sweden)

    Nancy Rodríguez C.

    2013-03-01

    Full Text Available Objetivo. Estimar algunos parámetros de estructura poblacional en una población Holstein del departamento de Antioquia. Materiales y métodos. El estudio se realizó con 427 vacas de la raza Holstein pertenecientes a 5 municipios del departamento de Antioquia. La genotipificación se llevó a cabo usando la técnica de PCR-PFLPs. La Heterocigocidad observada (Ho y Heterocigocidad esperada (He, la prueba de Hardy-Weinberg (HW y la estructura y diferenciación genética entre las poblaciones se calculó mediante los parámetros F de Wright, evaluados mediante el software GENEPOP. Las frecuencias alélicas y genotípicas se evaluaron con el método descrito por Hartl. Resultados. Las frecuencias genotípicas encontradas fueron 0.61, 0.34 y 0.05 para los genotipos AA, AB y BB respectivamente y las frecuencias de los alelos fueron 0.78 y 0.22 para A y B, encontrándose la población en equilibrio de HW. La heterocigocidad fue media entre poblaciones (Ho=0.368. Los valores FIS, FST y FIT de la población total fueron -0.0717, 0.0099 y -0.0611. Conclusiones. No fue posible asumir endogamia, ni exogamia en los municipios analizados, exceptuando el municipio de San Pedro de los Milagros, en cuyo caso se percibe de manera más fuerte el efecto del mejoramiento genético y la disminución de la heterocigocidad.

  9. gSeaGen: a GENIE-based code for neutrino telescopes

    CERN Document Server

    Distefano, Carla

    2016-01-01

    The gSeaGen code is a GENIE based application to generate neutrino-induced events in an underwater neutrino detector. The gSeaGen code is able to generate events induced by all neutrino flavours, taking into account topological differences between track-type and shower-like events. The neutrino interaction is simulated taking into account the density and the composition of the media surrounding the detector. The main features of gSeaGen will be presented together with some examples of its application within ANTARES and KM3NeT.

  10. Determinantes genéticos y ambientales de la experiencia de burlas : un estudio gemelar

    OpenAIRE

    Iranzo Tatay, Carmen

    2014-01-01

    Muchos aspectos de la conducta humana están significativamente influenciados por factores genéticos. Examinar la heredabilidad para una exposición ambiental, lo que se ha denominado correlación gen-ambiente o control genético para la exposición ambiental, es de interés para el campo de la salud mental porque proporciona conciencia sobre la naturaleza de la relación causal entre los humanos y su entorno social y físico. Nosotros planteamos que se puede evaluar la heredabilidad de las burlas pe...

  11. gSeaGen: A GENIE-based code for neutrino telescopes

    Directory of Open Access Journals (Sweden)

    Distefano Carla

    2016-01-01

    Full Text Available The gSeaGen code is a GENIE based application to generate neutrino-induced events in an underwater neutrino detector. The gSeaGen code is able to generate events induced by all neutrino flavours, taking into account topological differences between track-type and shower-like events. The neutrino interaction is simulated taking into account the density and the composition of the media surrounding the detector. The main features of gSeaGen will be presented together with some examples of its application within ANTARES and KM3NeT.

  12. Organismos modificados genéticamente en la alimentación humana

    OpenAIRE

    Barros Fernández, Paula

    2014-01-01

    El presente trabajo trata la controversia del tema de los organismos modificados genéticamente (OMG). Se mencionan los beneficios que aporta la ingeniería genética y también los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos modificados genéticamente, reportando casos de estudios que así lo constatan.Se tratan temas como seguridad alimentaria, legislación y normativas de etiquetado de estos nuevos alimentos, señalando su relación con la salud. Además, se ...

  13. Sobre el origen del código Genético

    OpenAIRE

    Fumiyoshi Watanabe; Michelle Robles; García, José A.

    2007-01-01

    Existen ciertas evidencias experimentales que sugieren un mundo primitivo en el que la molécula de ácido ribonucleico (RNA) era la molécula responsable de codificar la información genética y de catalizar un número limitado de reacciones. En este "mundo de RNA" pudo darse el origen del código genético actual. En este manuscrito se presenta una breve discusión sobre las principales teorías que se han propuesto sobre el origen del código genético.

  14. Genética y Probabilidad : pruebas de paternidad y portadores de enfermedades

    OpenAIRE

    2006-01-01

    La genética de poblaciones estudia la distribución de los genes en las poblaciones, así como los factores que mantienen o cambian la constitución genética de las mismas, de una generación a otra. Una población se define como un conjunto de individuos capaces de reproducirse entre sí, que coexisten geográfica y temporalmente, y constituyen una unidad evolutiva. El estudio de la genética de poblaciones es muy importante desde el punto de vista sanitario, ya que la mayoría de las enfermedades ti...

  15. Consideraciones genéticas sobre las dislipidemias y la aterosclerosis

    OpenAIRE

    Julio César Fernández Travieso

    2008-01-01

    La interacción entre factores genéticos y ambientales explican muchos aspectos de la aterosclerosis y las variaciones genéticas constituyen marcadores de riesgo de la enfermedad coronaria (EC), la cual ocupa el primer lugar entre las causas de morbilidad y mortalidad a nivel mundial. La predisposición familiar a padecer EC, junto al avance vertiginoso en técnicas de análisis de ADN y la disponibilidad de secuencias del genoma humano, han orientado la investigación de alteraciones genéticas re...

  16. The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force

    Science.gov (United States)

    2001-01-01

    The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force *EDITED by DR. RICHARD H. KOHN Editorial Abstract: Air...Force chief of staff Gen Ronald Fogleman’s early retirement in 1997 has caused great speculation. Was this a “resignation in protest”? Here for the...COVERED 00-00-2001 to 00-00-2001 4. TITLE AND SUBTITLE The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force 5a

  17. RAPDs na caracterização genético-molecular e no estudo da variabilidade genética de cultivares de ameixeira

    Directory of Open Access Journals (Sweden)

    Bianchi Valmor João

    2003-01-01

    Full Text Available Marcadores moleculares têm sido amplamente utilizados nas mais variadas espécies frutíferas para análise de "fingerprinting", para o processo de certificação de material vegetal e como ferramenta auxiliar em programas de melhoramento genético, para acessar a variabilidade genética entre genótipos. Dado a importância da cultura da ameixeira para a região Sul do Brasil, o presente trabalho teve por finalidade contribuir para a caracterização genético-molecular de 17 cultivares. As cultivares foram analisadas com 12 marcadores RAPD, que produziram 187 polimorfismos. O marcador OP A20 foi o mais polimórfico, produzindo 26 perfis diferentes. A análise de agrupamento, realizada com o método UPGMA, produziu um dendrograma que permitiu uma clara separação das cultivares em três grupos, correspondentes às suas respectivas espécies, Prunus salicina, Prunus domestica e Prunus cerasifera. O alto grau de polimorfismo detectado pelos marcadores RAPD confirma o potencial da técnica na análise de "fingerprinting" e sua utilidade na estimativa da variabilidade genética entre cultivares de ameixeira.

  18. La introducción de la genética en México: la genética aplicada al mejoramiento vegetal

    Directory of Open Access Journals (Sweden)

    Gaona Robles, Ana Lilia

    2001-12-01

    Full Text Available The aim of this paper is to deal with the introduction, sensu Conry, of genetics in Mexico. In the XIX Century the medical community was one of the most prominent groups that developed the field of «heredity», although they never used the genetical principles in their field. We will try to show that one of the main events in the history of genetics in Mexico deals with the introduction of techniques, rather than theories. The research in genetics began through one of the most practical applications, experimental genetics applied to the improvement of species with economical interests.

    El objetivo de este artículo es estudiar la introducción, sensu Conry, de la genética en México. Para ello, hablaremos de la comunidad médica del XIX, la que desarrolló los principios fundamentales de la «herencia», sin aplicar los principios elementales de la genética. Trataremos de mostrar cómo los principios genéticos fueron introducidos vía las técnicas y no vía la teoría. La investigación genética en México comenzó a través de sus aspectos más prácticos, la genética experimental aplicada al mejoramiento de las especies con intereses económicos.

  19. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia.Genética de la esperanza

    Directory of Open Access Journals (Sweden)

    Silvio Bolaños-Salvatierra

    2003-01-01

    Full Text Available En este documento se rebaten críticas hechas por Raventós y Jensen al artículo “Genética y comportamiento”. Cuatro temas fueron seleccionados: 1 se determina que los antipsicóticos aparecieron veinte años después de la concepción hereditaria de la esquizofrenia; 2 se considera que la discusión es altamente pertinente, para nada bizantina o irrelevante, debido que persisten prácticas epistémicas riesgosas en los investigadores genético-conductuales; 3 aunque ninguna conducta humana está exenta de influencia constitucional, el enfoque biologicista se ha propasado al pretender explicar genéticamente casi todo, desconfirmando solapadamente la importancia de la historia personal; y, 4 se plantea que la investigación biológica sobrevalora el peso de las anomalías genéticas frente a la historia social, por lo que solo aparenta cautela. Se propone investigar genéticamente la esperanza con el objetivo de saturar a la humanidad con ese tipo de explicaciones, para alcanzar más rápido una convivencia basada en la tolerancia y el respeto.

  20. Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

    Science.gov (United States)

    Gerritsen, Jacoline; Fuentes, Susana; Grievink, Wieke; van Niftrik, Laura; Tindall, Brian J; Timmerman, Harro M; Rijkers, Ger T; Smidt, Hauke

    2014-05-01

    A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1σ lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium, including Clostridium lituseburense (97.2%), Clostridium glycolicum (96.2%), Clostridium mayombei (96.2%), Clostridium bartlettii (96.0%) and Clostridium irregulare (95.5%). All these species show very low 16S rRNA gene sequence similarity (gen. nov., is proposed. The novel isolate CRIBT (=DSM 25109T=NIZO 4048T) is proposed as the type strain of the type species, Romboutsia ilealis gen. nov., sp. nov., of the proposed novel genus. It is proposed that C. lituseburense is transferred to this genus as Romboutsia lituseburensis comb. nov. Furthermore, the reclassification into novel genera is proposed for C. bartlettii, as Intestinibacter bartlettii gen. nov., comb. nov. (type species of the genus), C. glycolicum, as Terrisporobacter glycolicus gen. nov., comb. nov. (type species of the genus), C. mayombei, as Terrisporobacter mayombei gen. nov., comb. nov., and C. irregulare, as Asaccharospora irregularis gen. nov., comb. nov. (type species of the genus), on the basis of additional data collected in this study. In addition, an emendation of the species Peptostreptococcus anaerobius and the order Eubacteriales is provided.

  1. Desempenho agronômico e divergência genética de genótipos de coentro Agronomic performance and genetic divergence of coriander genotypes

    Directory of Open Access Journals (Sweden)

    Cândida Hermínia de Magalhães Bertini

    2010-09-01

    Full Text Available Cinco genótipos de Coriandrum sativum, procedentes de diferentes regiões do Estado do Ceará e duas cultivares comerciais, foram avaliados objetivando-se identificar genótipos com potencial agronômico para serem utilizados em programa de melhoramento genético por meio de análise de desempenho e divergência genética. O trabalho foi conduzido no período de setembro a dezembro de 2007 no município de Fortaleza-CE. O delineamento utilizado foi o aleatorizado em blocos com três repetições, onde os genótipos e cultivares foram considerados tratamentos. Na análise de desempenho verificou-se diferença entre as médias dos genótipos avaliados para as características: altura da planta, diâmetro do colo, surgimento da primeira inflorescência, antese, média de umbeletes/umbela, início e término do amadurecimento dos frutos. Entretanto, para as características agronômicas, número de folhas aproveitáveis e peso de cem frutos, não se verificou diferença entre as médias dos genótipos avaliados em relação às cultivares comerciais. Quanto à divergência, o genótipo 1, proveniente da região litorânea de Caucaia, foi o mais divergente, podendo ser usado em cruzamentos com os demais genótipos para a obtenção de populações segregantes. Os genótipos mais similares foram o Verdão-SF177 e o genótipo proveniente de Juazeiro. Os resultados do agrupamento não mostraram relação com as diferentes localizações geográficas dos genótipos avaliados.Five genotypes of coriander (Coriandrum sativum from different regions of Ceara-Brazil and two cultivars were assessed with the objective to identify genotypes with agronomic potential for use in breeding program using performance analysis and genetic divergence. The study was conducted during the period of September to December, 2007 in Fortaleza - Ceará. It was used a randomized blocks design with three replicates where the genotypes and cultivars were considered treatments. In

  2. Aspectos genéticos da obesidade Genetics of obesity

    Directory of Open Access Journals (Sweden)

    Iva Marques-Lopes

    2004-09-01

    Full Text Available A obesidade definida como a acumulação excessiva de gordura corporal deriva de um desequilíbrio crônico entre a energia ingerida e a energia gasta. Neste desequilíbrio podem estar implicados diversos fatores relacionados com o estilo de vida (dieta e exercício físico, alterações neuro-endócrinas, juntamente com um componente hereditário. O componente genético constitui um fator determinante de algumas doenças congênitas e um elemento de risco para diversas doenças crônicas como diabetes, osteoporose, hipertensão, câncer, obesidade, entre outras. O aumento da prevalência da obesidade em quase todos os países durante os últimos anos, parece indicar que existe uma predisposição ou susceptibilidade genética para a obesidade, sobre a qual atuam os fatores ambientais relacionados com os estilos de vida, em que se incluem principalmente os hábitos alimentares e a atividade física. A utilização de modelos animais de obesidade, a transferência génica e os estudos de associação e ligamento, permitiram a identificação de vários genes implicados na obesidade.Obesity, defined as an excessive body fat accumulation, is caused by a chronic imbalance between energy intake and energy expenditure. Several factors have been associated with this energy imbalance, such as life style (diet and physical activity, neuroendocrine disorders, together with the genetic background. The genetic background is a major determinant factor of some congenital diseases and a risk factor for some chronic disorders, such as diabetes, osteoporosis, hypertension, cancer, obesity, and others. The increased prevalence of obesity in most countries during the last years, seems to indicate that there is a genetic predisposition or susceptibility to be obese which is increased by environmental and life style factors, mainly by food habits and physical in activity. The use of obesity animal models, genetic transfer and the association and linkage studies

  3. GenASiS: General Astrophysical Simulation System. II. Nonrelativistic Hydrodynamics

    CERN Document Server

    Cardall, Christian Y; Endeve, Eirik; Mezzacappa, Anthony

    2012-01-01

    In this paper, the second in a series, we document the algorithms and solvers for compressible nonrelativistic hydrodynamics implemented in GenASiS (General Astrophysical Simulation System)---a new code being developed initially and primarily, though by no means exclusively, for the simulation of core-collapse supernovae. In the Mathematics division of GenASiS we introduce Solvers, which includes finite-volume updates for generic hyperbolic BalanceEquations and ordinary differential equation integration Steps. We also introduce the Physics division of GenASiS; this extends the Manifolds division of Mathematics into physical Spaces, defines StressEnergies, and combines these into Universes. We benchmark the hydrodynamics capabilities of GenASiS against many standard test problems; the results illustrate the basic competence of our implementation, demonstrate the manifest superiority of the HLLC over the HLL Riemann solver in a number of interesting cases, and provide preliminary indications of the code's abili...

  4. Ekspresi Gen CYP19 Aromatase, Estrogen, Androgen pada penderita Periodontitis Agresif

    Directory of Open Access Journals (Sweden)

    Dahlia Herawati

    2016-11-01

    Full Text Available Kepadatan tulang tubuh ditentukan oleh gen CYP19 aromatase, hormon estrogen dan androgen. Pada periodontitis agresif terjadi perkembangan cepat kerusakan tulang alveolar, dan kerusakan tulang alveoler tersebut tidak diimbangioleh regenerasi tulang. Tujuan penelitian ini adalah menunjukkan ekspresi gen CYP19 aromatase, estrogen, androgen pada penderita periodontitis agresif agar dapat untuk menjadi pertimbangan pada saat melakukan perawatan periodontal. Metode penelitian, pemeriksaan ekspresi gen aromatse CYP19 berasal dari spesimen tulang alveolar menggunakan imunohistokimia, pengukuran hormon estrogen dan androgen dari serum menggunakan Vidas: Elfa. Hasil penelitian ekspresi gene CYP19 aromatase pada periodontitis agresif menunjukkan gambaran lebih rendah densitasnya dibandingkan pada nonperiodontitis. Estrogen dan androgen pad aperiodontitis agresif ada kecenderungan lebih rendah dibandingkan pada nonperiodontitis. Kesimpulan regenerasi tulang alveoler pad a periodontitis agresif terhambat karena sedikitnya gen CYP19 aromatase dan hormon estrogen dan androgen yang berperan pada pembentukan tulang alveoler kurang memadai.

  5. On the new monotypic wolf spider genus Ovia gen. nov. (Araneae: Lycosidae, Lycosinae).

    Science.gov (United States)

    Sankaran, Pradeep M; Malamel, Jobi J; Sebastian, Pothalil A

    2017-01-17

    A new monotypic wolf spider genus, Ovia gen. nov. is proposed to accommodate a misplaced species: Pardosa procurva Yu & Song, 1988. Ovia procurva comb. nov. is redescribed, illustrated and designated as the type species for the genus. The subfamily placement of the new genus is discussed and it is considered as a member of Lycosinae Sundevall, 1833 and possibly closely related to Alopecosa Simon, 1885. The presence of an apical process (spur) on the median apophysis is proposed as the putative synapomorphy of Ovia gen. nov. The possible sister-taxon relationship of Ovia gen. nov. with Alopecosa is discussed and evidence on the occurrence of sexual dimorphism and mating plug within the genus are presented. Ovia gen. nov. is assumed to be of Holarctic origin, from which it has migrated to the Indomalayan region. Additionally, a current distribution map for the genus is provided.

  6. A Software-Assurance Design Approach for NextGen Enabling Technologies Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Next Generation Air Transportation System (NextGen) brings significant advancements to the current management of the National Airspace (NAS). These fundamental...

  7. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    Directory of Open Access Journals (Sweden)

    Juan Carlos Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  8. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    Directory of Open Access Journals (Sweden)

    Juan Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  9. KARAKTERISTIK SEKUEN cDNA PENGKODE GEN ANTI VIRUS DARI UDANG WINDU, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2016-11-01

    Full Text Available Transgenesis pada ikan merupakan sebuah teknik modern yang berpotensi besar dalam menghasilkan organisme yang memiliki karakter lebih baik melalui rekombinan DNA gen target termasuk gen anti virus dalam peningkatan resistensi pada udang. Gen anti virus PmAV (Penaeus monodon Anti Viral gene merupakan salah satu gen pengkode anti virus yang berasal dari spesies krustase. Penelitian ini dilakukan untuk mengetahui karakteristik gen anti virus yang diisolasi dari udang windu, Penaeus monodon. Isolasi gen anti virus menggunakan metode Polymerase Chain Reaction (PCR dan selanjutnya dipurifikasi untuk sekuensing. Data yang dihasilkan dianalisis dengan program Genetyx Versi 7 dan basic local alignment search tool (BLAST. Hasil penelitian menunjukkan bahwa gen anti virus PmAV yang berhasil diisolasi dari cDNA udang windu dengan panjang sekuen 520 bp yang mengkodekan 170 asam amino. BLAST-N menunjukkan tingkat similaritas yang sangat tinggi (100% dengan gen anti virus yang ada di GeneBank. Komposisi asam amino penyusun gen anti virus yang paling besar adalah serin (10,00%, sedangkan yang terkecil adalah asam amino prolin dan lisin masing-masing 1,76%. Analisis sekuen gen dan deduksi asam amino (BLAST-P memperlihatkan adanya C-type lectin-like domain (CTLD yang memiliki kemiripan dengan gen C-type lectin yang diisolasi dari beberapa spesies krustase. Transgenic fish technology is a potential modern technique in producing better character organism through DNA recombinant of target genes including anti viral gene for improvement of shrimp immunity. PmAV (Penaeus monodon Anti Viral gene is one of anti viral genes isolated from crustacean species. The research was conducted to analyze the characteristics anti viral gene isolated from tiger prawn, Penaeus monodon. Anti viral gene was isolated using Polymerase Chain Reaction (PCR technique and then purified for sequencing. Data obtained were analyzed using Genetyx Version 7 software and basic local alignment

  10. TRANSFER GEN ANTIVIRUS PADA EMBRIO UDANG WINDU, Penaeus monodon DALAM BERBAGAI KONSENTRASI DEOXYRIBO NUCLEIC ACID

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2011-12-01

    Full Text Available Teknologi transgenesis khususnya rekayasa genetik untuk menghasilkan udang windu resisten penyakit merupakan salah satu strategi yang dapat dilakukan dalam upaya pemecahan masalah penyakit yang menimpa budidaya udang windu. Teknologi transgenesis khususnya transfer gen antivirus pada udang windu telah berhasil dilakukan melalui teknik transfeksi. Meskipun demikian optimalisasi komponen teknologi tersebut masih perlu dilakukan. Konsentrasi DNA gen merupakan salah satu komponen teknologi transgenesis yang harus dioptimalkan untuk mendapatkan efisiensi dalam transfer gen. Penelitian bertujuan untuk mengetahui konsentrasi DNA gen antivirus yang optimal sebagai bahan transfer gen ke embrio menggunakan metode transfeksi. Embrio udang windu yang diperoleh dari hasil pemijahan induk asal Aceh, dikoleksi 5-10 menit setelah memijah dengan kepadatan 625 telur/2 mL. Transfeksi dilakukan dengan menggunakan media larutan transfeksi jetPEI dengan konsentrasi DNA gen antivirus sebagai perlakuan, yakni: 5, 10, dan 15 µg serta kontrol positif (tanpa plasmid DNA dan negatif (tanpa plasmid DNA dan larutan transfeksi, masing-masing 3 ulangan. Embrio hasil transfeksi ditetaskan pada stoples berisi air laut sebanyak 2 L yang diletakkan pada waterbath. Hasil penelitian menunjukkan bahwa gen antivirus telah berhasil diintroduksi ke embrio udang windu. Hasil analisis ragam menunjukkan bahwa perbedaan konsentrasi DNA (5-15 µg tidak berpengaruh nyata (P>0,05 terhadap daya tetas embrio udang windu. Analisis ekspresi gen pada larva udang windu juga menunjukkan adanya aktivitas ekspresi gen antivirus pada semua perlakuan konsentrasi DNA, di mana ekspresi gen antivirus pada larva transgenik lebih tinggi dibandingkan dengan kontrol (tanpa transfeksi. Sintasan pasca-larva PL-1 yang didapatkan pada penelitian ini adalah 12,0%; 10,0%; 10,6%; 12,3%; dan 14,2% masing-masing untuk perlakuan konsentrasi plasmid DNA 5 µg, 10 µg, 15 µg, kontrol positif dan negatif, di mana

  11. Micro-Trottoir à Genève à propos du CERN et du LHC

    CERN Multimedia

    CERN Video Productions

    2009-01-01

    Questions : Qu'est-ce-que le LHC? Quelle est la fonction du LHC? Qu'est-ce-que le CERN? D'après vous, les recherches engendrées pour le LHC peuvent-elles ?tre bénéfiques, utiles pour la société? Où a été inventé le Web? Lieux : Forum Meyrin Université de Genève Genève Centre

  12. Traffic Generator (TrafficGen) Version 1.4.2: Users Guide

    Science.gov (United States)

    2016-06-01

    2. Starting the Application 2 2.1 Prerequisites 2 2.2 Running TrafficGen 2 2.2.1 Linux 2 2.2.2 Mac OS X 2 2.2.3 Windows 2 3. Understanding the...scenarios in the examples directory. 2.2.1 Linux The following are steps to start TrafficGen on Linux . • Open a command prompt • Change directories to

  13. Stalacris Desutter-Grandcolas n. gen., an amazing cricket from South Africa (Orthoptera, Grylloidea, Phalangopsidae).

    Science.gov (United States)

    Desutter-Grandcolas, Laure

    2013-02-08

    Stalacris n. gen. (Insecta, Grylloidea, Phalangopsidae) is described from South Africa using characters of morphology and male genitalia. This taxon, known from only two species, Stalacris meridionalis n. gen., n. sp. and Stalacris sp, is characterized by the unique structure of its forewings. These are prolonged distally as long, acute and more or less articulated processes, forming a kind of forceps when forewings are opened.

  14. A 48-plex autosomal SNP GenPlex™ assay for human individualization and relationship testing

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Børsting, Claus; Morling, Niels

    2012-01-01

    SNPs are being increasingly used by forensic laboratories. Different platforms have been developed for SNP typing. We describe the GenPlex™ HID system protocol, a new SNP-typing platform developed by Applied Biosystems where 48 of the 52 SNPforID SNPs and amelogenin are included. The GenPlex™ HID...... system protocol has been successfully tested by a number of forensic laboratories using both ordinary and forensic samples....

  15. Cap a una ètica per a la genètica: fonamentació

    OpenAIRE

    Román Maestre, Begoña

    2002-01-01

    Voldria iniciar aquest article, plantejant dues qüestions bàsiques: perquè cal preguntar quina ètica per a la genètica, i perquè la genètica requereix d'una tal ètica. Pel que fa a la primera qüestió, quina ètica, cal aclarir que ha de ser una....

  16. Erections of Changa gen. nov. and Songga gen. nov. of Aolina (Hemiptera: Cicadidae: Cicadinae: Dundubiini) with taxonomic notes and a synonymization.

    Science.gov (United States)

    Lee, Young June

    2016-02-29

    Two new genera, Changa gen. nov. and Songga gen. nov., are erected in the subtribe Aolina Boulard, 2012, with the designations of Cosmopsaltria sita Distant, 1881 and Pomponia scitula Distant, 1888 as the type species, respectively. Khimbya sita (Distant, 1881) becomes Changa sita (Distant, 1881) comb. nov. Haphsa scitula (Distant, 1888) becomes Songga scitula (Distant, 1888) comb. nov. Haphsa jsguillotsi (Boulard, 2005) is transferred to Changa to become Changa jsguillotsi (Boulard, 2005) comb. nov. Haphsa stellata Lee, 2009 syn. nov. is synonymized with Changa sita. Haphsa sulaeyai (Boulard, 2005) is transferred to Macrosemia Kato, 1925 to become Macrosemia sulaeyai (Boulard, 2005) comb. nov. A revised key to the genera of Aolina is provided.

  17. Description of Teunomyces gen. nov. for the Candida kruisii clade, Suhomyces gen. nov. for the Candida tanzawaensis clade and Suhomyces kilbournensis sp. nov.

    Science.gov (United States)

    Kurtzman, Cletus P; Robnett, Christie J; Blackwell, Meredith

    2016-08-01

    DNA sequence analysis has shown that species of the Candida kruisii clade and species of the C. tanzawaensis clade represent phylogenetically circumscribed genera, which are described as Teunomyces gen. nov., type species T kruisii, and Suhomyces gen. nov., type species S tanzawaensis Many of the species are distributed worldwide and they are often isolated from fungus-feeding insects and their habitats. Included is the description of S. kilbournensis (type strain NRRL Y-17864, CBS 14276), a species found almost exclusively on maize kernels (Zea mays) in IL, USA.

  18. Saúde pública e ética na era da medicina genômica: rastreamentos genéticos

    OpenAIRE

    Bandeira,Flavia Miranda Gomes de Constantino; Gomes, Yara de Miranda; Abath,Frederico Guilherme Coutinho

    2006-01-01

    O presente artigo tem como objetivo contextualizar o campo da saúde pública diante dos grandes avanços da biotecnologia e genética aplicada, destacando elementos para a problematização do tema tais como benefícios e questões éticas relacionados aos rastreamentos genéticos. O Projeto Genoma Humano gerou várias expectativas, dentre elas, a possibilidade de rastrear genes associados a doenças e comportamentos, e mais ainda, de intervir geneticamente no ser humano, levantando preocupações relativ...

  19. Modelling of advanced structural materials for GEN IV reactors

    Science.gov (United States)

    Samaras, M.; Hoffelner, W.; Victoria, M.

    2007-09-01

    The choice of suitable materials and the assessment of long-term materials damage are key issues that need to be addressed for the safe and reliable performance of nuclear power plants. Operating conditions such as high temperatures, irradiation and a corrosive environment degrade materials properties, posing the risk of very expensive or even catastrophic plant damage. Materials scientists are faced with the scientific challenge to determine the long-term damage evolution of materials under service exposure in advanced plants. A higher confidence in life-time assessments of these materials requires an understanding of the related physical phenomena on a range of scales from the microscopic level of single defect damage effects all the way up to macroscopic effects. To overcome lengthy and expensive trial-and-error experiments, the multiscale modelling of materials behaviour is a promising tool, bringing new insights into the fundamental understanding of basic mechanisms. This paper presents the multiscale modelling methodology which is taking root internationally to address the issues of advanced structural materials for Gen IV reactors.

  20. Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce.

    Science.gov (United States)

    Tanasupawat, Somboon; Thongsanit, Jaruwan; Thawai, Chitti; Lee, Keun Chul; Lee, Jung-Sook

    2011-07-01

    Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced l-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 °C and in the presence of 10 % (w/v) NaCl. The dominant fatty acid was C(18 : 1)ω9c. The DNA G+C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain C01(T) was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9 %. The strains could be distinguished clearly from D. incerta DSM 20581(T) based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01(T) ( = KCTC 13150(T)  = TISTR 1958(T)  = PCU 316(T)).

  1. De compras por el supermercado genético

    Directory of Open Access Journals (Sweden)

    Singer, Peter

    2002-12-01

    Full Text Available Should we say that selective abortion is a “powerful message that we seek to eliminate future persons”? When deaf parents want to have a deaf children, Are they crazy? Are they advocates of a culture –the Deaf culture? If genetic engineering can give us health, intelligent, and athletic childrens, why say not to this advantages? The aim of this article is not to deal with all objections that could be urged against these options; the purpose is developing a clear understanding of the central values at stake.

    ¿Estamos eliminando una cultura, la de los sordos, cuando tratamos de evitar que nazcan niños sordos? El aborto terapéutico, ¿significa que, por ejemplo, creemos que las vidas de los afectados por síndrome de Down son vidas de menor valor que las vidas “normales”? Si se permitiera la manipulación genética de los embriones ¿sería poco ético encargar hijos guapos y altos? Este artículo no aporta respuestas a estas preguntas, pero sí que plantea los términos para dar cuenta de ellas y eleva acta de lo difícil que es dar una respuesta concluyente.

  2. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier d...

  3. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet - 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier ...

  4. Testes genéticos na eqüideocultura

    Directory of Open Access Journals (Sweden)

    Eduardo Geraldo Alves Coelho

    2008-07-01

    Full Text Available Nos últimos anos a equideocultura deu um salto qualitativo, havendo hoje, no mercado, animais de alto valor e geneticamente superiores. Isso é possível, em grande parte, devido aos avanços na área da genética animal, os quais permitem identificar, não apenas anomalias, mas também diversos genes de interesse econômico. Com o auxílio da citogenética pode-se identificar indivíduos com alterações no número ou na estrutura dos cromossomos, o que em muitos casos afeta principalmente a reprodução. Também a confirmação de genealogia, anteriormente feita por tipagem sangüínea e atualmente por testes de DNA, tem papel extremamente importante, não apenas por garantir a ascendência dos animais, mas também porque um pedigree confiável pode permitir ao criador identificar a origem de problemas genéticos em seu rebanho e reduzi-los ou mesmo, eliminá-los. Ainda com as ferramentas da biologia molecular, podemos hoje, identificar indivíduos que apresentam genes desejáveis ou indesejáveis, o que nos permite selecioná-los precocemente, reduzindo assim, os custos do produtor e aumentando o valor agregado dos animais. Entre tais genes podemos destacar os que identificam portadores ou afetados por mutações genéticas indesejáveis como: SCID (Síndrome da Imunodeficiência Combinada, HYPP (Parilisa Hipercalêmica, HERDA (Astenia Dérmica Regional Hereditária Eqüina, etc. Também a identificação dos genes que determinam a cor ou padrão da pelagem já pode ser feita direta ou indiretamente (por meio de marcadores genéticos, como é o caso dos genes para as pelagens Overo, Tobiano, etc. Com os avanços no estudo do genoma eqüino muito mais estará disponível em breve, o que certamente só trará maiores contribuições à equideocultura mundial.In the last few years the horse breeding industry is achieving significant progresses producing animals of high commercial value and genetically superior. It was possible, mainly due to

  5. GenFamClust: an accurate, synteny-aware and reliable homology inference algorithm.

    Science.gov (United States)

    Ali, Raja H; Muhammad, Sayyed A; Arvestad, Lars

    2016-06-04

    Homology inference is pivotal to evolutionary biology and is primarily based on significant sequence similarity, which, in general, is a good indicator of homology. Algorithms have also been designed to utilize conservation in gene order as an indication of homologous regions. We have developed GenFamClust, a method based on quantification of both gene order conservation and sequence similarity. In this study, we validate GenFamClust by comparing it to well known homology inference algorithms on a synthetic dataset. We applied several popular clustering algorithms on homologs inferred by GenFamClust and other algorithms on a metazoan dataset and studied the outcomes. Accuracy, similarity, dependence, and other characteristics were investigated for gene families yielded by the clustering algorithms. GenFamClust was also applied to genes from a set of complete fungal genomes and gene families were inferred using clustering. The resulting gene families were compared with a manually curated gold standard of pillars from the Yeast Gene Order Browser. We found that the gene-order component of GenFamClust is simple, yet biologically realistic, and captures local synteny information for homologs. The study shows that GenFamClust is a more accurate, informed, and comprehensive pipeline to infer homologs and gene families than other commonly used homology and gene-family inference methods.

  6. Diaminobutyricibacter tongyongensis gen. nov., sp. nov. and Homoserinibacter gongjuensis gen. nov., sp. nov. belong to the family Microbacteriaceae.

    Science.gov (United States)

    Kim, Soo-Jin; Ahn, Jae-Hyung; Weon, Hang-Yeon; Hamada, Moriyuki; Suzuki, Ken-ichiro; Kwon, Soon-Wo

    2014-06-01

    Two bacterial strains, KIS66-7(T) and 5GH26-15(T), were isolated from soil samples collected in the South Korean cities of Tongyong and Gongju, respectively. Both strains were aerobic, Gram-stain-positive, mesophilic, flagellated, and rodshaped. A phylogenetic analysis revealed that both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence of strain KIS66-7(T) had the highest similarities with those of Labedella gwakjiensis KSW2-17(T) (97.3%), Cryobacterium psychrophilum DSM 4854T (97.2%), Leifsonia lichenia 2Sb(T) (97.2%), Leifsonia naganoensis JCM 10592(T) (97.0%), and Cryobacterium mesophilum MSL-15(T) (97.0%). Strain 5GH26-15(T) showed the highest sequence similarities with Leifsonia psychrotolerans LI1T (97.4%) and Schumannella luteola KHIAT (97.1%). The 16S rRNA gene sequence from KIS66-7(T) exhibited 96.4% similarity with that from 5GH26-15(T). Strain KIS66-7(T) contained a B2γ type peptidoglycan structure with D-DAB as the diamino acid; MK-13, MK-12, and MK-14 as the respiratory quinones; ai-C15:0, ai-C17:0, and i-C16:0 as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Strain 5GH26-15T had a B2β type peptidoglycan structure with D-DAB as the diamino acid; MK-14 and MK-13 as the respiratory quinones; ai-C15:0, i-C16:0, and ai-C{vn17:0} as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Both strains had low DNA-DNA hybridization values (gen. nov., sp. nov. (type strain KIS66-7(T)=KACC 15515(T)=NBRC 108724(T)) and Homoserinibacter gongjuensis gen. nov., sp. nov. (type strain 5GH26-15(T)=KACC 15524(T)=NBRC 108755(T)) within the family Microbacteriaceae.

  7. Enfermedades genéticas más frecuentes en pacientes atendidos en consulta de genética clínica

    Directory of Open Access Journals (Sweden)

    Elibett Carcasés Carcasés

    2015-02-01

    Full Text Available La estimación de la prevalencia de las enfermedades genéticas se dificulta, entre otras causas, por su rareza. Se realizó un estudio descriptivo retrospectivo, para identificar las enfermedades genéticas de mayor prevalencia en pacientes atendidos por este programa en el Centro Provincial de Genética Médica de Las Tunas, Cuba; desde el año 1989 hasta julio de 2014. Se revisaron todas las historias clínicas. Predominó el origen monogénico (69 %, siendo los síndromes dismórficos los más numerosos y diversos, entre ellos los neurocutáneos, que representaron el 35 %. La enfermedad genética monogénica con mayor número de casos fue la Neurofibromatosis I con el 14,4 % y el 22,2 % de las enfermedades eran de origen monogénico y dismórfico. La Trisomía 21 representó el 77 % de la causa cromosómica. En el origen multifactorial prevalecieron los defectos congénitos mayores, entre ellos los defectos reductivos de miembros (27 %

  8. Critical Issues on Materials for Gen-IV Reactors

    Energy Technology Data Exchange (ETDEWEB)

    Caro, M; Marian, J; Martinez, E; Erhart, P

    2009-02-27

    Within the LDRD on 'Critical Issues on Materials for Gen-IV Reactors' basic thermodynamics of the Fe-Cr alloy and accurate atomistic modeling were used to help develop the capability to predict hardening, swelling and embrittlement using the paradigm of Multiscale Materials Modeling. Approaches at atomistic and mesoscale levels were linked to build-up the first steps in an integrated modeling platform that seeks to relate in a near-term effort dislocation dynamics to polycrystal plasticity. The requirements originated in the reactor systems under consideration today for future sources of nuclear energy. These requirements are beyond the present day performance of nuclear materials and calls for the development of new, high temperature, radiation resistant materials. Fe-Cr alloys with 9-12% Cr content are the base matrix of advanced ferritic/martensitic (FM) steels envisaged as fuel cladding and structural components of Gen-IV reactors. Predictive tools are needed to calculate structural and mechanical properties of these steels. This project represents a contribution in that direction. The synergy between the continuous progress of parallel computing and the spectacular advances in the theoretical framework that describes materials have lead to a significant advance in our comprehension of materials properties and their mechanical behavior. We took this progress to our advantage and within this LDRD were able to provide a detailed physical understanding of iron-chromium alloys microstructural behavior. By combining ab-initio simulations, many-body interatomic potential development, and mesoscale dislocation dynamics we were able to describe their microstructure evolution. For the first time in the case of Fe-Cr alloys, atomistic and mesoscale were merged and the first steps taken towards incorporating ordering and precipitation effects into dislocation dynamics (DD) simulations. Molecular dynamics (MD) studies of the transport of self

  9. Electronic and magnetic properties of CrGen (15 ⩽ n ⩽ 29) clusters: A DFT study

    Science.gov (United States)

    Mahtout, Sofiane; Tariket, Yacine

    2016-06-01

    We report ab initio calculations of electronic and magnetic properties of medium-sized CrGen (15 ⩽ n ⩽ 29) clusters using density functional theory. The encapsulation of Cr atoms within Gen clusters leads to stable Cr encapsulated Gen clusters. The binding energies generally increase while the differences between the highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO-LUMO gaps) generally decrease with the increasing of cluster size. The clusters of CrGen at size 16, 17, 19, 22, 24 and 29 exhibit high stabilities when compared to their neighbors. This has been discussed in terms of their structures, energies and the effect of the position of doping atom. Doping of Gen clusters with one Cr atom leads to CrGen clusters with magnetic moment depending on the structure of the clusters and the position of Cr atom in the clusters. Moreover, vertical ionization potential, vertical electronic affinity, and chemical hardness are also analyzed.

  10. Mejoramiento genético vegetal in vitro

    Directory of Open Access Journals (Sweden)

    Antonia Gutiérrez M.

    2003-01-01

    Full Text Available El mejoramiento genético de plantas es una de las hazañas más antiguas del hombre, que inició con la domesticación de las mismas bajo condiciones controladas y la selección de aquellas capaces de proporcionar una mejor fuente de alimentos. Esto marcó una de las fases más importantes en el progreso de la humanidad, al permitirle transitar de una vida nómada e individualista a una sociedad organizada y cooperativista. Dicho mejoramiento fue fortuito y lento y permaneció como un arte y no como una ciencia hasta principios del siglo XX, luego de que las llamadas leyes de Mendel, pioneras en la explicación de los procesos de la herencia, obtuvieron reconocimiento (Briggs y Knowles, 1967. El proceso que emplea fitomejoradores ha creado un sinnúmero de variedades de plantas con el objeto de incrementar su producción, resistencia a plagas y enfermedades, y la adaptación a ambientes específicos, regiones y usos, mediante la selección de variedades cultivadas localmente, cruzadas entre sí o con las de otras áreas, o también con plantas silvestres que tengan los genes deseados. Sin embargo, obtener plantas mejoradas por estos medios resulta difícil en ocasiones por lo que se recurre a otros métodos para producir variantes útiles, tales como la selección celular, la variación somaclonal y las mutaciones inducidas, entre otros

  11. Haloactinomyces albus gen. nov., sp. nov., isolated from Dead Sea.

    Science.gov (United States)

    Lai, Hangxian; Jiang, Yingying; Chen, Xiu; Li, Qinyuan; Jiang, Chenglin; Jiang, Yi; Wei, Xiaomin

    2017-01-10

    A novel halophilic, filamentous actinomycete strain, designated AFM 10258T, was isolated from a sediment sample collected from Dead Sea of Israel. The isolate grew with 10-35% NaCl and did not grow without NaCl. The isolate formed white aerial mycelium and long spore chains, and two spores were separated by sterile mycelium. The spores were non-motile, spherical and rugose-surfaced. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. Major fatty acids were anteiso-C17:0, iso-C16:0 and iso-C15:0. MK-9(H4) was the predominant menaquinone and the DNA G+C content was 62.8 mol%. 16S rRNA gene sequence analysis indicated that strain AFM 10258T shared low sequence similarity with the closely related representatives of the families Pseudonocardiaceae (below 94.47%) and Actinopolysporaceae (below 93.76%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AFM 10258T formed a robust clade with members of the family Actinopolysporaceae. On the basis of analysis of phenotypic, chemical and molecular characteristics, strain AFM 10258T is considered to represent a novel species of a new genus , for which the name Haloactinomyces albus gen. nov., sp. nov., is proposed. The type strain is AFM 10258T (=DSM 45977T = CGMCC 4.7115T).

  12. Genética em transtornos alimentares: ampliando os horizontes de pesquisa

    Directory of Open Access Journals (Sweden)

    Pinheiro Andréa Poyastro

    2006-01-01

    Full Text Available OBJETIVO: Revisar a literatura atual concernente à pesquisa genética em transtornos do comportamento alimentar e discutir questões relevantes ao desenvolvimento de um projeto de pesquisa genética nessa área no Brasil. MÉTODO: A revisão realizada utilizou a base de dados Medline, no período de 1984 a maio de 2005, com os seguintes termos de busca: "anorexia nervosa", "bulimia nervosa", "eating disorders", "binge eating disorder", "family studies", "twin studies", "molecular genetics studies". RESULTADOS: Os dados atuais apontam para uma contribuição relevante dos fatores genéticos na suscetibilidade à anorexia e à bulimia nervosa. A pesquisa genética com populações miscigenadas deve levar em consideração o tamanho da amostra, a densidade de genotipagem e a estratificação populacional. Através de "admixture mapping" é possível estimar a estrutura genética destas populações e localizar genes relacionados à variação étnica de doenças ou traços de interesse. CONCLUSÕES: O desenvolvimento de uma grande iniciativa de colaboração em genética de transtornos alimentares no Brasil e na América Latina viabilizará estudar os fatores genéticos em transtornos do comportamento alimentar no contexto de grupos inter-étnicos, e integrar uma nova perspectiva biológica à etiologia destes distúrbios.

  13. Salud pública, genética y ética

    Directory of Open Access Journals (Sweden)

    Kottow Miguel H

    2002-01-01

    Full Text Available La investigación genética ha tenido una enorme expansión en recientes décadas, con repercusiones terapéuticas aún inciertas. El análisis bioético tradicional de las complejas prácticas genéticas ha sido insuficiente por sostenerse en la ética de la investigación y en la bioética de corte principialista. Los problemas éticos más importantes de la genética son de orden colectivo y deben ser abordados por una reflexión ético-social cuyo enfoque es más amplio que la agenda interpersonal del principialismo. Temas como exploraciones genéticas, cuestiones patrimoniales, manipulación génica y asignación de recursos, deben todos ser sometidos a un pensamiento inspirado en los requerimientos de la ciudadanía, en el bien común y en la definición del rol del Estado en fiscalizar actividades genéticas y en proteger a la población. El objetivo del estudio es mostrar cómo el amplio campo de la ética y de la genética tiene una mayor relevancia en el campo social que en el clínico. El objetivo del trabajo es señalar que la bioética principialista ha enfatizado los problemas éticos individuales que nacen con la intervención genética, a costa de marginar sus importantes repercusiones sociales.

  14. Salud pública, genética y ética

    Directory of Open Access Journals (Sweden)

    Miguel H Kottow

    2002-10-01

    Full Text Available La investigación genética ha tenido una enorme expansión en recientes décadas, con repercusiones terapéuticas aún inciertas. El análisis bioético tradicional de las complejas prácticas genéticas ha sido insuficiente por sostenerse en la ética de la investigación y en la bioética de corte principialista. Los problemas éticos más importantes de la genética son de orden colectivo y deben ser abordados por una reflexión ético-social cuyo enfoque es más amplio que la agenda interpersonal del principialismo. Temas como exploraciones genéticas, cuestiones patrimoniales, manipulación génica y asignación de recursos, deben todos ser sometidos a un pensamiento inspirado en los requerimientos de la ciudadanía, en el bien común y en la definición del rol del Estado en fiscalizar actividades genéticas y en proteger a la población. El objetivo del estudio es mostrar cómo el amplio campo de la ética y de la genética tiene una mayor relevancia en el campo social que en el clínico. El objetivo del trabajo es señalar que la bioética principialista ha enfatizado los problemas éticos individuales que nacen con la intervención genética, a costa de marginar sus importantes repercusiones sociales.

  15. Wave Data Processing and Analysis, Part 2: Codes for Coupling GenCade and CMS-Wave

    Science.gov (United States)

    2013-09-01

    coastal modeling system , Report 2: CMS -Wave. ERDC/CHL-TR-11-10. Vicksburg, MS: US Army Engineer Research and Development Center. Connell, K. J. and...Coupling GenCade and CMS -Wave by Rusty Permenter, Kenneth J. Connell, and Zeki Demirbilek PURPOSE: This Coastal and Hydraulics Engineering...to GenCade. This is the second CHETN in a two‐part series detailing the process of coupling CMS ‐Wave with GenCade. This CHETN focuses on

  16. Papel de ginA y ginB en la movilidad de la isla genómica GInt

    OpenAIRE

    Echeverría Ancín, Myriam

    2014-01-01

    La transferencia horizontal de genes, mediada por elementos genéticos móviles tales como las islas genómicas, es uno de los principales mecanismos de generación de variabilidad genética en bacterias, permitiendo su adaptación a nuevos nichos ecológicos. En el grupo de Patología Vegetal de la UPNA se ha descrito, en especies de Pseudomonas, una nueva familia de islas genómicas, denominadas GInt. Éstas se caracterizan por contener en su extremo 5’ el operón gin, constituido ...

  17. La Dislexia del Desarrollo: Gen, Cerebro y Cognición Developmental Dyslexia: Gen, Brain, and Cognition

    Directory of Open Access Journals (Sweden)

    Alberto M Galaburda

    2006-11-01

    Full Text Available La dislexia del desarrollo es un trastorno que se caracteriza por dificultades en el aprendizaje de la lectura. Recientemente se ha podido vincular la dislexia a cuatro distintos genes candidatos de riesgo: DYX1C1, KIAA0319, DCDC2 y ROBO1. Estos cuatro genes participan en el desarrollo cerebral, y anomalías de dicho desarrollo constituyen los elementos conocidos del cuadro biológico que subyace a la dislexia. En animales experimentales, la inducción de anomalías del desarrollo cerebral similares produce problemas en el procesamiento de ciertos sonidos. En humanos, problemas de procesamiento de sonidos semejantes se asocian a un trastorno de aprendizaje de la lectura. Por consiguiente, es posible por primera vez, trazar una trayectoria tentativa entre una característica genética, variaciones del desarrollo del cerebro, y trastornos conductuales y cognitivos asociados a la dislexia.Developmental dyslexia is a disorder characterized by difficulties in reading acquisition. Recently, dyslexia has been related to four different genes which are prone-risk candidates: DYX1C1, KIAA0319, DCDC2, and ROBO1. These four genes participate in brain development, and anomalies in that development comprise the known elements of the biological constellation underlying dyslexia. The induction of similar brain development anomalies in experimental animals produces problems in the processing of certain sounds. In humans, similar sound processing problems are related to a reading acquisition disorder. Consequently, for the first time it is possible to delineate a tentative path between a genetic characteristic, brain development variations, and behavioral and cognitive disorders related to dyslexia.

  18. Biometria e armazenamento de sementes de genótipos de cacaueiro

    Directory of Open Access Journals (Sweden)

    Lucimara Ribeiro Venial

    2017-03-01

    Full Text Available Genótipos de Theobroma cacao L. devem ser melhor estudados, para se identificar aqueles que produzem sementes mais desenvolvidas e viáveis após o armazenamento. Objetivou-se com este trabalho estudar a biometria e dois tempos de armazenamento de sementes de genótipos de cacaueiro. A biometria foi avaliada em oito genótipos de cacaueiro (tratamentos. Foram instalados testes de germinação em delineamento inteiramente ao acaso, no esquema fatorial 8 x 2 (genótipos: CCN51, PH16, CEPEC2002, Ipiranga, SJ02, PS1319, TSH1188 e Comum x dois períodos de armazenamento: 0 e dois dias. O genótipo TSH1188 apresentou maior comprimento, relação comprimento/largura, espessura e massa de 100 sementes. A absorção de água das sementes recém-colhidas dos genótipos é lenta, justificada pelos altos teores de água, o que não caracteriza padrão-trifásico. Os teores de água reduziram em média 2,3 vezes nas sementes armazenadas em relação às recém-colhidas. A germinação das sementes recém-colhidas dos genótipos foi de 100%. Após o armazenamento, as sementes do PS1319 apresentaram a menor redução da germinação (39%, enquanto as dos PH16, CEPEC2002 e SJ02 reduziram 96%. A velocidade de germinação foi maior e o tempo médio menor que dois dias nas sementes recém-colhidas do PS1319, indicando serem mais tolerante à dessecação. Sugere-se o uso dos genótipos TSH1188 e PS1319 em programas de melhoramento genético.

  19. ATLAS Versus NextGen Model Atmospheres: A Combined Analysis of Synthetic Spectral Energy Distributions

    Science.gov (United States)

    Bertone, E.; Buzzoni, A.; Chávez, M.; Rodríguez-Merino, L. H.

    2004-08-01

    We carried out a critical appraisal of the two theoretical models, Kurucz' ATLAS9 and PHOENIX/NextGen, for stellar atmosphere synthesis. Our tests relied on the theoretical fit of spectral energy distributions (SEDs) for a sample of 334 target stars along the whole spectral-type sequence, from the classical optical catalogs of Gunn & Stryker and Jacoby et al. The best-fitting physical parameters (Teff, logg) of stars allowed an independent calibration of the temperature and bolometric scale versus empirical classification parameters (i.e., spectral type and MK luminosity class); in addition, the comparison of the synthetic templates from the ATLAS and NextGen grids allowed us to probe the capability of the models to match spectrophotometric properties of real stars and assess the impact of the different input physics. We can sketch the following main conclusions of our analysis: (1) Fitting accuracy of both theoretical libraries drastically degrades at low Teff at which both ATLAS and NextGen models still fail to properly account for the contribution of molecular features in the observed SED of K-M stars. (2) Compared with empirical calibrations, both ATLAS and NextGen fits tend, on average, to predict slightly warmer (by 4%-8%) Teff for both giant and dwarf stars of fixed spectral type, but ATLAS provides, in general, a sensibly better fit (a factor of 2 lower σ of flux residuals) than NextGen. (3) There is a striking tendency of NextGen to label target stars with an effective temperature and surface gravity higher than that of ATLAS. The effect is especially evident for MK I-III objects for which about one in four stars is clearly misclassified by NextGen in logg. This is a consequence of some ``degeneracy'' in the solution space, partly induced by the different input physics and geometry constraints in the computation of the integrated emerging flux (ATLAS model atmospheres assume standard plane-parallel layers, while NextGen adopts, for low-gravity stars, a

  20. Variabilidad genética y gestión forestal

    Directory of Open Access Journals (Sweden)

    R. Alía

    2003-01-01

    Full Text Available La diversidad biológica, su conservación e incremento, es uno de los principios básicos de la gestión forestal sostenible. La alta variabilidad genética de las especies forestales es responsable de los procesos de adaptación ante factores bióticos y abióticos extremos que, a su vez, aseguran la persistencia frente a los riesgos a los que están sometidas las masas forestales. En este trabajo se analizan distintos aspectos relacionados con la variabilidad genética de las especies forestales, y como la gestión forestal influye sobre ella. Se analiza la variabilidad entre poblaciones (que es una de las bases de la comercialización de material forestal de reproducción, la influencia del sistema de reproducción en la variabilidad genética de las masas y los efectos en ellas de los tratamientos selvícolas, así como la variación individual y clonal aprovechada en los programas de selección. Por último, se analiza el papel de la variabilidad genética en los programas de mejora y conservación de recursos genéticos forestales

  1. ProGenExpress: Visualization of quantitative data on prokaryotic genomes

    Directory of Open Access Journals (Sweden)

    Watson Michael

    2005-04-01

    Full Text Available Abstract Background The integration of genomic information with quantitative experimental data is a key component of systems biology. An increasing number of microbial genomes are being sequenced, leading to an increasing amount of data from post-genomics technologies. The genomes of prokaryotes contain many structures of interest, such as operons, pathogenicity islands and prophage sequences, whose behaviour is of interest during infection and disease. There is a need for simple and novel tools to display and analyse data from these integrated datasets, and we have developed ProGenExpress as a tool for visualising arbitrarily complex numerical data in the context of prokaryotic genomes. Results Here we describe ProGenExpress, an R package that allows researchers to easily and quickly visualize quantitative measurements, such as those produced by microarray experiments, in the context of the genome organization of sequenced prokaryotes. Data from microarrays, proteomics or other whole-genome technologies can be accurately displayed on the genome. ProGenExpress can also search for novel regions of interest that consist of groups of adjacent genes that show similar patterns across the experimental data set. We demonstrate ProGenExpress with microarray data from a time-course experiment involving Salmonella typhimurium. Conclusion ProGenExpress can be used to visualize quantitative data from complex experiments in the context of the genome of sequenced prokaryotes, and to find novel regions of interest.

  2. PERAN GEN AIM1 DAN INTENSITAS CAHAYA TERHADAP KARAKTER POLA PIGMEN IKAN BADUT HITAM (Amphiprion percula

    Directory of Open Access Journals (Sweden)

    Daniar Kusumawati

    2012-08-01

    Full Text Available Pola pigmen merupakan faktor utama yang menentukan tingginya kualitas ikan hias. Pada benih-benih F1 populasi budidaya ikan badut hitam (Amphiprion percula diketahui mengalami degeneratif pola pigmen yang menyimpang dibandingkan dengan populasi di alam. Pola pigmen merupakan salah satu karakter fenotip yang diturunkan di mana ekspresinya bergantung pada interaksi genetik dengan faktor eksternal yaitu lingkungan. Tujuan dari penelitian ini adalah untuk mengkaji peran gen yaitu Aim1 yang mengontrol sintesis melanin dan kondisi lingkungan dalam menginduksi pola pigmen hitam ikan badut hitam (Amphiprion percula. Analisis gen Aim1 dilakukan dengan menggunakan program speedy PCR dilanjutkan dengan SSCP (single strand confirmation polymorphism untuk mengidentifikasi variasi genetik dari gen Aim1. Analisis SDS Page dilakukan untuk mengetahui peranan lingkungan terhadap profil protein yang disintesis. Berdasarkan hasil penelitian diketahui bahwa primer Aim1 memiliki sisi pengenalan pada whole genom ikan badut hitam (Amphiprion percula pada target sequence 45 bp. Berdasarkan analisis SSCP profil fragmentasi amplicon primer Aim1 pada masing-masing tingkatan pada populasi budidaya homolog dengan populasi alam, sehingga gen Aim1 bukan merupakan gen yang mengontrol fenomena degeneratif pola pigmen ikan badut hitam (Amphiprion percula. Peran lingkungan yaitu cahaya memberikan pengaruh positif dalam menginduksi pola pigmen melalui stimulus pada sistem neuron dan migrasi melanophore.

  3. Diversidade genética molecular de progênies de dendezeiro

    Directory of Open Access Journals (Sweden)

    Crystianne Bentes Barbosa Ferreira

    2012-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a diversidade genética, entre e dentro de progênies de dendezeiro tipo dura, de origem Deli. A caracterização genética foi feita com uso de marcadores microssatélites em 24 progênies usadas na produção comercial de sementes, sendo 22 provenientes de autofecundação e duas de cruzamentos entre irmãos completos. Foi realizada análise de variância molecular entre e dentro das progênies, com posterior construção de um dendrograma. Observou-se baixa variabilidade genética nas progênies, com média de 1,32 alelos por loco e variância genética total igual a 0,3241. A maior parte da variação ocorreu entre progênies. A menor variabilidade genética dentro das progênies pode ser explorada nos cruzamentos com progênies endogâmicas de outras origens, o que facilitaria o alcance de heterose para o desenvolvimento de novas variedades.

  4. Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage.

    Science.gov (United States)

    Lee, Shun-Hsiao; Princz, Lissa Nicola; Klügel, Maren Felizitas; Habermann, Bianca; Pfander, Boris; Biertümpfel, Christian

    2015-12-18

    Holliday junctions (HJs) are key DNA intermediates in homologous recombination. They link homologous DNA strands and have to be faithfully removed for proper DNA segregation and genome integrity. Here, we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 Å resolution. The GEN1 core is similar to other Rad2/XPG nucleases. However, unlike other members of the superfamily, GEN1 contains a chromodomain as an additional DNA interaction site. Chromodomains are known for their chromatin-targeting function in chromatin remodelers and histone(de)acetylases but they have not previously been found in nucleases. The GEN1 chromodomain directly contacts DNA and its truncation severely hampers GEN1's catalytic activity. Structure-guided mutations in vitro and in vivo in yeast validated our mechanistic findings. Our study provides the missing structure in the Rad2/XPG family and insights how a well-conserved nuclease core acquires versatility in recognizing diverse substrates for DNA repair and maintenance.

  5. Mechanism of Holliday junction resolution by the human GEN1 protein.

    Science.gov (United States)

    Rass, Ulrich; Compton, Sarah A; Matos, Joao; Singleton, Martin R; Ip, Stephen C Y; Blanco, Miguel G; Griffith, Jack D; West, Stephen C

    2010-07-15

    Holliday junction (HJ) resolution is essential for chromosome segregation at meiosis and the repair of stalled/collapsed replication forks in mitotic cells. All organisms possess nucleases that promote HJ resolution by the introduction of symmetrically related nicks in two strands at, or close to, the junction point. GEN1, a member of the Rad2/XPG nuclease family, was isolated recently from human cells and shown to promote HJ resolution in vitro and in vivo. Here, we provide the first biochemical/structural characterization of GEN1, showing that, like the Escherichia coli HJ resolvase RuvC, it binds specifically to HJs and resolves them by a dual incision mechanism in which nicks are introduced in the pair of continuous (noncrossing) strands within the lifetime of the GEN1-HJ complex. In contrast to RuvC, but like other Rad2/XPG family members such as FEN1, GEN1 is a monomeric 5'-flap endonuclease. However, the unique feature of GEN1 that distinguishes it from other Rad2/XPG nucleases is its ability to dimerize on HJs. This functional adaptation provides the two symmetrically aligned active sites required for HJ resolution.

  6. Aspectos genéticos y neuroendocrinos en el trastorno del espectro autista

    Directory of Open Access Journals (Sweden)

    Norma Oviedo

    2015-01-01

    Full Text Available El autismo, hoy en día definido como trastornos del espectro autista, fue descrito inicialmente en 1943. Se caracteriza por alteraciones en la comunicación, la interacción social y un espectro restringido de intereses del paciente. Generalmente se identifica en etapas tempranas del desarrollo a partir de los 18 meses de edad. Actualmente el autismo se considera un desorden neurológico con un espectro que abarca diferentes grados que se asocian con factores genéticos, no genéticos y del medio ambiente. Dentro de los factores genéticos se han referido diversos síndromes relacionados con este trastorno. Asimismo, el autismo se ha estudiado a nivel genético, neurofisiológico, neuroquímico y neuropatológico. Las técnicas de neuroimagen han mostrado múltiples anormalidades estructurales en estos pacientes. También se han observado alteraciones relacionadas en los sistemas serotoninérgico, GABAérgico, catecolaminérgico y colinérgico. En este trabajo se presenta una actualización de la información de los aspectos genéticos y neuroendocrinos del trastorno del espectro autista.

  7. Jurados ciudadanos y organismos genéticamente modificados

    Directory of Open Access Journals (Sweden)

    Luque, Emilio

    2005-04-01

    Full Text Available Apolitical sociology of food must look into the processes whereby regulatory decisions on food are made, and also into the democratic potential of their alternatives. Five "experiments in democracy" are described, in which a promising deliberative device has been used: citizens' juries. Indian and Brazilian peasants, on the one hand, and British consumers in the other took part in them, and they focused on Genetically Modified Organisms. These processes, whose defining trait is granting jurors access to expert witnesses presented by all stakeholders in the controversy, show the impressive ability of ordinary citizens to articulate their analysis of complex issues, a reassessment of risks, and a recontextualization of the use of GMOs. At any rate, deliberative democracy is not a magic bullet for the epistemic and political crisis that underlies food crisis; instead, it points at a paradigm change towards an experimental democratic polity in which the instances of representation of the public and publics are multiplied.

    Una sociología política de la alimentación debe analizar los procesos a través de los cuales se alcanzan las decisiones de regulación y control alimentario y examinar el potencial de sus alternativas. Se describen aquí cinco experimentos democráticos en los que se ha empleado uno de los dispositivos deliberativos más prometedores en condiciones de alta complejidad cognitiva, los llamados jurados ciudadanos, con la participación de campesinos hindúes y brasileños y consumidores británicos, y centrados en los Organismos Genéticamente Modificados. Estos procesos, caracterizados por el acceso de los miembros del jurado a testigos expertos presentados por los distintos participantes en la controversia, muestran la enorme capacidad de los ciudadanos "de a pie" para articular el análisis de un problema complejo, reevaluar sus riesgos y recontextualizar el uso de los OGM. En todo caso, la democracia deliberativa no es

  8. Fluviicoccus keumensis gen. nov., sp. nov., isolated from freshwater.

    Science.gov (United States)

    Kim, Min-Kyeong; Kim, Tae-Woon; Kim, Tae-Su; Joung, Yochan; Han, Ji-Hye; Kim, Seung Bum

    2016-01-01

    A Gram-stain-negative, non-spore-forming and coccus-shaped bacterial strain, designated 4DR5T, was isolated from freshwater and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10-40 °C (optimum 30 °C), at pH 6-9 (optimum pH 7) and in the presence of 0-0.4 % (w/v) NaCl (optimum 0 %) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain 4DR5T was assigned to the family Moraxellaceae of the class Gammaproteobacteria, and its closest related taxa were species of the genera Perlucidibaca (93.67 % sequence similarity), Agitococcus (93.07 %), Paraperlucidibaca (92.31-92.38 %), Alkanindiges (91.79 %) and Acinetobacter (90.24-91.23 %). The predominant isoprenoid quinone detected in strain 4DR5T was Q-10. The major cellular fatty acids were a summed feature consisting of C16 : 1ω7c and/or C16 : 1ω6c, one consisting of C18 : 1ω7c and/or C18 : 1ω6c, and C16 : 0. The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of the strain was 61.2 mol%. The phylogenetic, chemotaxonomic and biochemical data not only supported the affiliation of strain 4DR5T to the family Moraxellaceae, but also separated it from other established genera within the family. Therefore, the novel isolate evidently represents a novel species of a new genus of Moraxellaceae, for which the name Fluviicoccus keumensis gen. nov., sp. nov. is proposed. The type strain of Fluviicoccus keumensis is 4DR5T ( = KCTC 32475T = JCM 19370T).

  9. HIV gp120 vaccine - VaxGen: AIDSVAX, AIDSVAX B/B, AIDSVAX B/E, HIV gp120 vaccine - Genentech, HIV gp120 vaccine AIDSVAX - VaxGen, HIV vaccine AIDSVAX - VaxGen.

    Science.gov (United States)

    2003-01-01

    VaxGen is developing prophylactic vaccines against HIV-1 consisting of two recombinant gp120 surface proteins from different HIV-1 strains.This profile has been selected from R&D Insight, a pharmaceutical intelligence database produced by Adis International Ltd. The bivalent vaccines [AIDSVAX B/B and AIDSVAX B/E] are being evaluated in two phase III trials. The first multicentre phase III trial of AIDSVAX B/B, was conducted principally in Canada and the US but also at some sites in the Netherlands and Puerto Rico. The trial was completed at the end of 2002. The second phase III trial is being conducted in Thailand with the AIDSVAX B/E vaccine. VaxGen announced in July 2002 that it would be delaying its Biologics License Application (BLA) for AIDSVAX until 2004 to enable the company to fulfil pre-approval manufacturing requirements. AIDSVAX is based on an earlier monovalent gp120 vaccine developed by Genentech that was shown to be safe in humans. VaxGen (formerly Genenvax) was formed as a spin-off company from Genentech with the sole purpose of developing the gp120 vaccine. VaxGen announced in July 2002 that the original License and Supply agreement with Genentech, signed in May 1997, had been amended. Under the revised agreement, Genentech maintains its right to market and sell AIDSVAX in North America, but has relinquished its options to commercialise the vaccine candidate in the rest of the world. Genentech's earlier decision to waive its option to manufacture AIDSVAX has also been formalised in this agreement. Additionally, VaxGen's royalty payments to Genentech for sales to the WHO or UN for underdeveloped nations have also been reduced by up to 50% and Genentech has extended the milestone date associated with VaxGen submitting an NDA. A $US120 million joint venture (Celltrion) has been formed between VaxGen and South Korean investors to manufacture more than 200 million doses of AIDSVAX a year. Celltrion will build and operate two biotechnology manufacturing

  10. La incidencia de la genética en el derecho (humano al matrimonio

    Directory of Open Access Journals (Sweden)

    Sergio Romeo Malanda

    2014-10-01

    Full Text Available La idea de evitar el nacimiento de persona con algún tipo de enfermedad ha sido una constante en la historia de la humanidad. Una de las formas de conseguirlo ha sido la de establecer limitaciones a la procreación, tanto directamente, a través de esterilizaciones forzosas, como indirectamente, prohibiendo matrimonios entre personas que no cumplían los requisitos estimados más deseables para tener descendencia sana.Contenido: Introducción. El derecho al matrimonio. Derecho al matrimonio y genética: los diagnósticos genéticos prematrimoniales. Conclusión: los diagnósticos genéticos prematrimoniales respetuosos con los derechos humanos.

  11. La incidencia de la genética en el derecho (humano al matrimonio

    Directory of Open Access Journals (Sweden)

    Sergio Romeo Malanda

    2014-10-01

    Full Text Available La idea de evitar el nacimiento de persona con algún tipo de enfermedad ha sido una constante en la historia de la humanidad. Una de las formas de conseguirlo ha sido la de establecer limitaciones a la procreación, tanto directamente, a través de esterilizaciones forzosas, como indirectamente, prohibiendo matrimonios entre personas que no cumplían los requisitos estimados más deseables para tener descendencia sana.Contenido: Introducción. El derecho al matrimonio. Derecho al matrimonio y genética: los diagnósticos genéticos prematrimoniales. Conclusión: los diagnósticos genéticos prematrimoniales respetuosos con los derechos humanos.

  12. INCORPORACIÓN DEL GEN Mi A VARIEDADES DE TOMATE MEDIANTE EL MARCADOR Aps-1

    OpenAIRE

    2006-01-01

    Frente a los nematodos del género Meloidogyne es la resistencia genética, junto a métodos de lucha integrada, la opción más recomendada para el tomate, por lo que los cultivares e híbridos modernos portan, en su mayoría, el gen Mi, que confiere resistencia no completa a varias especies del género Meloidogyne. El Instituto Nacional de Ciencias Agrícolas (INCA), con la colaboración de la Estación Experimental La Mayora-CSIC, España, ha desarrollado un programa de mejora genética, con el objetiv...

  13. Magnetic behavior in Cr2@Gen (1≤n≤12) clusters: A density functional investigation

    Science.gov (United States)

    Dhaka, Kapil; Trivedi, Ravi; Bandyopadhyay, Debashis

    2014-04-01

    With a goal to produce magnetic moment in Cr2 Doped Gen clusters which will be useful for practical applications, we have considered the structure and magnetic properties of Pure Germanium clusters and substitutionally doped it with Cr dimer to produce Cr2@Gen clusters. As the first step of calculation, geometrical optimizations of the nanoclusters have been done. These optimized geometries have been used in calculate the average binding energy per atom (BE), HOMO-LUMO gap and hence the relative stability of the clusters. These parameters have been demonstrated as structural and electronic properties of the clusters. Gap between highest occupied molecular orbital and lowest unoccupied molecular orbital indicate cluster to be a potential motif for generating magnetic cluster assembled materials. Based on these values a comparative study on different sized clusters has been done in order to understand the origin of structures, electronic and magnetic properties of Cr2@Gen nanoclusters.

  14. Biomek®-3000 and GenPlex in Forensic Genetics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Tomas Mas, Carmen; Hansen, Anders Johannes;

      SNP genotyping provides a supplement for conventional STR-based kits currently used for human identification. GenPlex (Applied Biosystems) is a SNP genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement and valid......  SNP genotyping provides a supplement for conventional STR-based kits currently used for human identification. GenPlex (Applied Biosystems) is a SNP genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement...... with the Biomek-3000 were compared to results obtained with 15 samples that were processed manually. Subsequently, a total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared to those obtained from the same samples using the SNaPshot® single base...

  15. Tools for Designing, Evaluating, and Certifying NextGen Technologies and Procedures: Automation Roles and Responsibilities

    Science.gov (United States)

    Kanki, Barbara G.

    2011-01-01

    Barbara Kanki from NASA Ames Research Center will discuss research that focuses on the collaborations between pilots, air traffic controllers and dispatchers that will change in NextGen systems as automation increases and roles and responsibilities change. The approach taken by this NASA Ames team is to build a collaborative systems assessment template (CSAT) based on detailed task descriptions within each system to establish a baseline of the current operations. The collaborative content and context are delineated through the review of regulatory and advisory materials, policies, procedures and documented practices as augmented by field observations and interviews. The CSAT is developed to aid the assessment of key human factors and performance tradeoffs that result from considering different collaborative arrangements under NextGen system changes. In theory, the CSAT product may be applied to any NextGen application (such as Trajectory Based Operations) with specified ground and aircraft capabilities.

  16. How Does Mentoring Contribute to Gen Y Employees’ Intention to Stay? An Indian Perspective

    Science.gov (United States)

    Naim, Mohammad Faraz; Lenka, Usha

    2017-01-01

    The present study is aimed at investigating the impact of mentoring on intention to stay of Gen Y employees working in Indian IT industry. Also, the mediating roles of perceived organization support and affective commitment are examined. Primary data were collected from a sample of 314 Gen Y employees (born between 1980-2000) from IT industry in Delhi, NCR India. Data analysis was carried out using AMOS and SPSS to test sequential mediation. Findings reveal that mentoring has a direct influence on intention to stay of Gen Y employees and perceived organization support and affective commitment sequentially mediate the relationship between the two. This study contributes to the literature on mentoring, perceived organization support, affective commitment, and intention to stay. PMID:28580029

  17. El problema del discurso acerca de la individualidad genética humana

    OpenAIRE

    Ordóñez Vásquez, Adriana

    2008-01-01

    Se abordó el problema filosófico acerca de la individualidad genética humana, desde un discurso ontológico. Determinándose un problema lógico, epistemológico, semiológico y ontológico, al mismo tiempo que genético. Se presenta el paralelismo entre rasgos de la individualidad señalados por la tradición filosófica clásica y su posible reformulación del discurso desde la genética humana actual. Prueba la pertinencia semiótica de Peirce - biosemiótica en el desarrollo del problema y propone un mo...

  18. rMotifGen: random motif generator for DNA and protein sequences

    Directory of Open Access Journals (Sweden)

    Hardin C Timothy

    2007-08-01

    Full Text Available Abstract Background Detection of short, subtle conserved motif regions within a set of related DNA or amino acid sequences can lead to discoveries about important regulatory domains such as transcription factor and DNA binding sites as well as conserved protein domains. In order to help assess motif detection algorithms on motifs with varying properties and levels of conservation, we have developed a computational tool, rMotifGen, with the sole purpose of generating a number of random DNA or protein sequences containing short sequence motifs. Each motif consensus can be user-defined, randomly generated, or created from a position-specific scoring matrix (PSSM. Insertions and mutations within these motifs are created according to user-defined parameters and substitution matrices. The resulting sequences can be helpful in mutational simulations and in testing the limits of motif detection algorithms. Results Two implementations of rMotifGen have been created, one providing a graphical user interface (GUI for random motif construction, and the other serving as a command line interface. The second implementation has the added advantages of platform independence and being able to be called in a batch mode. rMotifGen was used to construct sample sets of sequences containing DNA motifs and amino acid motifs that were then tested against the Gibbs sampler and MEME packages. Conclusion rMotifGen provides an efficient and convenient method for creating random DNA or amino acid sequences with a variable number of motifs, where the instance of each motif can be incorporated using a position-specific scoring matrix (PSSM or by creating an instance mutated from its corresponding consensus using an evolutionary model based on substitution matrices. rMotifGen is freely available at: http://bioinformatics.louisville.edu/brg/rMotifGen/.

  19. O futuro da epidemiologia genética de características complexas

    Directory of Open Access Journals (Sweden)

    Feitosa Mary F.

    2002-01-01

    Full Text Available A epidemiologia genética evoluiu de um enfoque em estudos sobre doenças mendelianas raras para a análise genética de características complexas. Com o advento de informações sobre a completa seqüência de genes ao longo do genoma humano e de outros organismos, o interesse da epidemiologia genética em desvendar a natureza dos fatores que influenciam essas características se tornou primordial. São apresentados os principais métodos empregados no estudo de doenças complexas bem como suas principais vantagens e desvantagens. Discute-se a importância na determinação da amostra e o uso de fenótipos e marcadores genéticos apropriados. Como exemplo das estratégias citadas tomamos o estudo de índice de massa corporal (BMI para ilustrar um fator genético principal localizado no cromossomo 7. Em uma discussão sobre tendências no estudo de ligação, embora reconhecendo que famílias e genealogias continuarão sendo o foco principal das amostras, discute-se alguns novos e eficientes tipos de amostragem (como por exemplo, controles não-relacionados em que amostras de conjunto de DNA serão universalmente empregadas. O reconhecimento da heterogeneidade genética entre estudos e sua interpretação será uma das mais importantes características no futuro das análises de características complexas.

  20. Loci asociados con enfermedades genéticas y calidad de carne en bovinos Charolais Mexicanos

    Directory of Open Access Journals (Sweden)

    Ana María Sifuentes Rincón

    2015-01-01

    Full Text Available Se determinaron las frecuencias alélicas y genotípicas de ocho marcadores localizados en los genes calpaína (CAPN, 4 751 y 316, calpastatina (CASTT1 y tiroglobulina (TG5, asociados a calidad de carne, y en los genes, m iost atina (MSTN, Q204X, arginino succinato sintasa (ASS, monofosfato sintasa (UMPS y miofosforilasa (PYGM, asocia dos a enfermedade s genéticas de ganado bovino. Se muestrearon 493 animales Charolais de registro de dos hatos ubicado s en Sonora (n=157 y tres en Nuevo León (n=336. No se encontraron portadores de los alelos T-ASS y T-UM PS, pero sí portadores del alelo Q204X del gen MSTN en frecuencias de  1 % en las poblaciones de Sonora y de 8.6 a 14.4 % en las de Nuevo León. Además, se identificaron portadores del marcador del gen PYGM, en frecuencias del 6.5 y de 1.0 % para un hato de Sonora y otro de Nuevo León, respectivamente. El análisis de diferenciación génica p areado entre las poblaciones y con los cuatro loci mostró que hay diferencias altamente significativas dentro de pobl aciones del noroeste ( P <0.0001 y entre éstas y las del noreste ( P <0.001, la cual es explicada principalmente por los loci CAPN-316 y TG5. De acuerdo a los resultados obtenidos se recomienda el monitoreo del marcador del gen PYGM y del ale lo Q204X del gen MSTN, así como también implementar estrategias para confirmar la utilidad de los marcadores asociado s a calidad y productividad como herramienta para complementar los progra mas de mejoramiento genético.

  1. VARIABILIDAD DEL GEN NUCLEAR G3PDH EN JATROPHA CURCAS L.

    Directory of Open Access Journals (Sweden)

    Sonia Castro Guzmán

    2015-11-01

    Full Text Available Jatrophacurcas L.es una especie nativa de América tropical; en nuestro país se ha venido utilizando principalmente como medicinal y alimenticia desde la época prehispánica. Actualmente el aceite extraído de sus semillas ha adquirido importancia internacional ya que puede ser transformado a biodiesel. El conocimiento que existe sobre la variabilidad genética de la especie es escaso. Un gen utilizado con éxito para el estudio de patrones de variación y origen de la yuca (ManihotesculentaL. y del caco (Theobromacacao L. es el gen nuclearG3pdh(Gliceraldehído3 fosfato deshidrogenasa involucrado en la fotosíntesis. Con base en ello, en este trabajo se exploró la variabilidad del gen G3pdh en individuos deJ. curcasprovenientes de 15 poblaciones de los estados de Veracruz, Campeche, Yucatán y Quintana Roo. Para obtener el gen G3pdh completo de alrededor de 1000 pares de bases, se amplificó utilizando losprimersdiseñados porStrandet al. (1997 y los dosprimersinternos reverse diseñados porOlsenySchaal(1999 para obtener segmentos más cortos, de 600 y 800 pares de bases aproximadamente. Por primera vez se amplificaron aproximadamente 500pby los resultados demuestran que el gen G3pdh es útil para analizar la variabilidad deJ. curcas, y con un importante potencial para evaluar la distribución y evolución de sus poblaciones en México, conocer las relaciones ancestro descendiente a nivel poblacional y explicar las causas de la distribución de los distintoshaplotipos.

  2. SIPSMetGen: It's Not Just For Aircraft Data and ECS Anymore.

    Science.gov (United States)

    Schwab, M.

    2015-12-01

    The SIPSMetGen utility, developed for the NASA EOSDIS project, under the EED contract, simplified the creation of file level metadata for the ECS System. The utility has been enhanced for ease of use, efficiency, speed and increased flexibility. The SIPSMetGen utility was originally created as a means of generating file level spatial metadata for Operation IceBridge. The first version created only ODL metadata, specific for ingest into ECS. The core strength of the utility was, and continues to be, its ability to take complex shapes and patterns of data collection point clouds from aircraft flights and simplify them to a relatively simple concave hull geo-polygon. It has been found to be a useful and easy to use tool for creating file level metadata for many other missions, both aircraft and satellite. While the original version was useful it had its limitations. In 2014 Raytheon was tasked to make enhancements to SIPSMetGen, this resulted a new version of SIPSMetGen which can create ISO Compliant XML metadata; provides optimization and streamlining of the algorithm for creating the spatial metadata; a quicker runtime with more consistent results; a utility that can be configured to run multi-threaded on systems with multiple processors. The utility comes with a java based graphical user interface to aid in configuration and running of the utility. The enhanced SIPSMetGen allows more diverse data sets to be archived with file level metadata. The advantage of archiving data with file level metadata is that it makes it easier for data users, and scientists to find relevant data. File level metadata unlocks the power of existing archives and metadata repositories such as ECS and CMR and search and discovery utilities like Reverb and Earth Data Search. Current missions now using SIPSMetGen include: Aquarius, Measures, ARISE, and Nimbus.

  3. Genealogia e heredogramas como ferramentas no ensino da genética mendeliana

    OpenAIRE

    Manzke, Vítor; Bobrowski,Vera Lucia; Manzke, Gabriela; Rodrigues,Maria José

    2012-01-01

    Este trabalho foi desenvolvido a partir do uso da genealogia como ferramenta auxiliar ao estudo da genética mendeliana nos contextos do mono e do diíbridismo. Para isso os alunos passaram a estabelecer relações entre o grau de parentesco dos membros de seu grupo familiar e a identificar a manifestação de características genéticas pré-estabelecidas em comum acordo entre o professor e os alunos. Nosso objetivo foi oportunizar aos alunos a sistematização do grupo familiar, em cinco gerações, ten...

  4. Análisis del gen SOX-2 en carcinoma escamoso de cabeza y cuello

    OpenAIRE

    González Márquez, Rocío

    2015-01-01

    El carcinoma escamoso de cabeza y cuello es la sexta neoplasia más frecuente en el mundo. El origen de dichos tumores implica gran numero de alteraciones genéticas y moleculares, siendo recientemente estudiado el gen SOX2. Se estudiaron las muestras procedentes de 220 pacientes con carcinoma escamoso de hipofaringe, laringe y senos paranasales. En el estudio inmunohistoquímico apareció un grado de sobreexpresión similar en tumores faringeos y laríngeos, así como relación significativa con...

  5. Análisis del gen SOX-2 en carcinoma escamoso de cabeza y cuello

    OpenAIRE

    González Márquez, Rocío

    2014-01-01

    El carcinoma escamoso de cabeza y cuello es la sexta neoplasia más frecuente en el mundo. El origen de dichos tumores implica gran numero de alteraciones genéticas y moleculares, siendo recientemente estudiado el gen SOX2. Se estudiaron las muestras procedentes de 220 pacientes con carcinoma escamoso de hipofaringe, laringe y senos paranasales. En el estudio inmunohistoquímico apareció un grado de sobreexpresión similar en tumores faringeos y laríngeos, así como relación significativa con...

  6. Introgresión genética (IG en ganado criollo colombiano (GCC

    Directory of Open Access Journals (Sweden)

    Zuluaga FN.

    2001-06-01

    Full Text Available Determinar variabilidad y estimar los niveles de mestizaje en las razas de GCC mediante laevaluación de marcadores polimórficos en ADN mitocondrial y cromosoma Y y analizar filo-genéticamente el origen del GCC.Las siete razas de GCC han sufrido procesos de adaptación a las condiciones tropicales y hanacumulado características genéticas únicas y de gran valor, principalmente de tipo reproductivoe inmunológico.

  7. Avances genéticos y moleculares en el estudio de trastornos mentales

    OpenAIRE

    Daisy Natalia Salamanca-Ortíz; Jorge Yamit Vergara-Vergara; Franklin Escobar-Córdoba; Álvaro Rodríguez-Gama; Jorge Eduardo Caminos- Pinzón

    2014-01-01

    Actualmente, con el aumento de la disponibilidad de técnicas para la realización de estudios genéticos, han aparecido nuevas áreas del conocimiento como la epigenética y la farmacogenética. Estas nuevas áreas han permitido esclarecer las bases genéticas implicadas en la aparición de las enfermedades que afectan al ser humano. Dada su aplicación global, la psiquiatría no ha sido ajena al alcance de estas nuevas disciplinas, lo cual se refleja en el gran número de estudios realizados a nivel mu...

  8. LabSystem Gen, a tool for structuring and analyzing genetic data in histocompatibility laboratories.

    Science.gov (United States)

    Sousa, Luiz Cláudio Demes da Mata; dos Santos Neto, Pedro de Alcântara; de Souza, Fernando da Fonseca; do Monte, Semiramis Jamil Hadad

    2012-04-01

    Analysis of HLA data involves queries on web portals, whose search parameters are data stored in laboratories' databases. In order to automate these queries, one approach is to structure laboratory data into a database and to develop bioinformatic tools to perform the data mapping. In this context, we developed the LabSystem Gen tool that allows users to create a Laboratory Information System, without programming. Additionally we implemented a framework that provides bioinformatic tools, transparent access to public HLA (human leukocyte antigen) information resources. We demonstrated the LabSystemGen system by implementing BMDdb, which is a LIMS that manages data of recipients and donors of organ transplant.

  9. 不是木马的“木马”——VBS:Obfuscated-gen [Trj

    Institute of Scientific and Technical Information of China (English)

    陈小虎

    2009-01-01

    今天,单位上网网速奇慢,一会儿就断线,打开网页avast弹出提示发现病毒“VBS:Obfuscated—gen[Trj]”,打开任何一个网页,都出现“VBS:Obfuscated—gen[Trj]”的病毒提示,很多网页根本就打不开。其他同事也反映上网出现病毒提示,网速慢得吓人……

  10. LEPINOCONUS CHIOCCHINII GEN. N., N. SP., A CONICAL AGGLUTINATED FORAMINIFERA FROM THE UPPER CRETACEOUS OF ITALY

    Directory of Open Access Journals (Sweden)

    ERZIKA CRUZ-ABAD

    2017-04-01

    Full Text Available A new conical agglutinated foraminifer, Lepinoconus chiocchinii gen n., n. sp. from the lower Campanian shallow-water platform deposits of the Lepini Mountains (central Apennines, Italy, is described. It has a pseudo-keriothecal wall structure, uniserial arrangement of the adult chambers and multiple apertures. The exoskeleton is constituted by beams (main and intercalary continuous from one chamber to the next, while the endoskeleton bears pillars. The new taxon is included in the Coskinolinidae family. Lepinoconus chiocchinii gen. n., n. sp. is known from southern Italy, Greece and Albania.

  11. MLPA en el estudio de desórdenes genómicos

    OpenAIRE

    Ramírez, Jésica; Echeverría, María; Mampel, Alejandra; Marino, Miguel; Gallardo, A.; Triguy, J.; Schroh, A.; Arce, Cecilia; Marzese, Diego; Calderón, Enrique; Vargas, Ana

    2009-01-01

    El término de desórdenes genómicos se utiliza para definir aquellas condiciones que surgen por inestabilidad en la molécula de ADN y, que ocasionan, rearreglos cromosómicos que involucran regiones de uno o varios pares de megabases. Estos rearreglos determinan la pérdida, ganancia o disrupción de genes cuya expresión fenotípica varía de acuerdo a la cantidad de secuencia codificante presente (dosage- sensitive- genes). Estas anormalidades genó...

  12. Biochemical and molecular characterization of the gentisate transporter GenK in Corynebacterium glutamicum.

    Directory of Open Access Journals (Sweden)

    Ying Xu

    Full Text Available BACKGROUND: Gentisate (2,5-dihydroxybenzoate is a key ring-cleavage substrate involved in various aromatic compounds degradation. Corynebacterium glutamicum ATCC13032 is capable of growing on gentisate and genK was proposed to encode a transporter involved in this utilization by its disruption in the restriction-deficient mutant RES167. Its biochemical characterization by uptake assay using [(14C]-labeled gentisate has not been previously reported. METHODOLOGY/PRINCIPAL FINDINGS: In this study, biochemical characterization of GenK by uptake assays with [(14C]-labeled substrates demonstrated that it specifically transported gentisate into the cells with V(max and K(m of 3.06 ± 0.16 nmol/min/mg of dry weight and 10.71 ± 0.11 µM respectively, and no activity was detected for either benzoate or 3-hydoxybenzoate. When GenK was absent in strain RES167 ΔgenK, it retained 85% of its original transport activity at pH 6.5 compared to that of strain RES167. However, it lost 79% and 88% activity at pH 7.5 and 8.0, respectively. A number of competing substrates, including 3-hydroxybenzoate, benzoate, protocatechuate and catechol, significantly inhibited gentisate uptake by more than 40%. Through site-directed mutagenesis, eight amino acid residues of GenK, Asp-54, Asp-57 and Arg-386 in the hydrophobic transmembrane regions and Arg-103, Trp-309, Asp-312, Arg-313 and Ile-317 in the hydrophilic cytoplasmic loops were shown to be important for gentisate transport. When conserved residues Asp-54 and Asp-57 respectively were changed to glutamate, both mutants retained approximately 50% activity and were able to partially complement the ability of strain RES167 ΔgenK to grow on gentisate. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that GenK is an active gentisate transporter in Corynebacterium glutamicum ATCC13032. The GenK-mediated gentisate transport was also shown to be a limiting step for the gentisate utilization by this strain. This enhances our

  13. Riesgos sobre la salud de los alimentos modificados genéticamente: una revision bibliografica

    OpenAIRE

    2000-01-01

    A lo largo de 1999, se ha venido intensificando el debate sobre la seguridad de los alimentos modificados genéticamente, una importante y compleja área de investigación científica, la cual demanda unos estándares rigurosos. Diversos grupos, incluyendo asociaciones de consumidores y Organizaciones no Gubernamentales (ONGs) han sugerido que todos los alimentos modificados genéticamente deberían ser sometidos a estudios a largo plazo con animales antes de su aprobación para el consumo humano. El...

  14. A psicologia face aos novos progressos da genética humana

    OpenAIRE

    Rossano André Dal-Farra; Emerson Juliano Prates

    2004-01-01

    Os avanços da Genética contemporânea têm suscitado uma grande polêmica a respeito da gênese do homem e das possibilidades de agir sobre a biologia humana, repercutindo intensamente sobre os mais diversos ramos do conhecimento. Considerando a importância do tema, este texto pretende apresentar de forma sucinta os principais resultados de pesquisas sobre as bases genéticas do comportamento humano, com o foco voltado para a importância do profissional de Psicologia em questões relativas ao assun...

  15. Alimentos Transgénicos : Organismos Genéticamente Modificados (OGM)

    OpenAIRE

    Martín López, Jimena

    2016-01-01

    Los alimentos transgénicos son aquellos que proceden de un organismo modificado genéticamente. La introducción de este tipo de productos en nuestra dieta es un tema que genera controversia ya que en muchos casos no se conoce con exactitud los efectos que esta modificación puede tener en el ser humano. A lo largo de las páginas de este trabajo se explica la historia de la aparición de estos organismos gracias a procedimientos de ingeniería genética, en los que se modifican fragmentos de su ADN...

  16. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  17. Estrategias de genética reversa aplicadas al vesivirus de conejo

    OpenAIRE

    García Manso, Alberto

    2016-01-01

    En el ámbito de la Virología, se denomina genética reversa al proceso de rescate de virus RNA de novo a partir de una copia del genoma viral en forma de cDNA. El trabajo recogido en esta memoria de tesis doctoral se ha dirigido a la obtención de un sistema de genética reversa para el vesivirus de conejo (RaV), uno de los pocos calicivirus capaz de ser propagado en cultivos celulares. Para ello, se han utilizado diferentes estrategias para expresar distintos clones de cDNA sintetizados. En ...

  18. Régimen de propiedad sobre recursos genéticos y conocimiento tradicional

    OpenAIRE

    2011-01-01

    Este artículo expone los cambios históricos que se han dado en relación con la propiedad, el control y el acceso a los recursos genéticos. Desde su consideración como patrimonio común de la humanidad, los derechos sobre el material y la información genética han sufrido profundas transformaciones. El marco jurídico internacional concerniente a estos derechos se ha transformado para legitimar y promover su apropiación y monopolización. Parte de esta transformación fue la expansión de derechos d...

  19. Ingeniería genómica mediante sistemas CRISPR-Cas

    OpenAIRE

    López Mancheño, Yaiza Araceli

    2015-01-01

    Desde su descubrimiento, a finales de la década de los ochenta, hasta la actualidad, las secuencias denominadas CRISPR, se han revelado como instrumentos a través de los cuáles provocar cambios dirigidos en secuencias génicas de interés. El conocimiento de su función y actuación natural en microorganismos permitió a los científicos, sospechar sus posibles aplicaciones como herramienta de edición genómica ya que el sistema era capaz de reconocer elementos genéticos externos, incorporarlos y, t...

  20. Análisis genético del virus peruano de la fiebre amarilla

    OpenAIRE

    Carlos Yábar V; Yván Campos B; Kelly Quispe T; Carlos Carrillo P; Ysabel Montoya P

    2002-01-01

    Objetivo: Determinar las variantes genéticas de aislamientos del virus peruano de la Fiebre Amarilla (FA). Materiales y métodos: la región carboxiterminal del gen de la envoltura (E) de cinco aislamientos de FA obtenidas de pacientes provenientes de Ayacucho 1978 (PER1), Junín 1995 (PER2), Cerro de Pasco (PER3), Cusco (1998) y San Martín (1999) fue amplificada por PCR, secuenciada y analizada con programas software de ADN. Resultados: el índice de similaridad de la secuencia de nucleótidos en...

  1. Ilegalidad de la investigación genética en colombia

    OpenAIRE

    Gómez, Diana; Nemogá Soto, Gabriel

    2013-01-01

    El marco legislativo colombiano que regula el acceso a los recursos genéticos y el uso de la diversidad biológica con fines investigativos se ha convertido en un obstáculo para el desarrollo de los procesos investigativos en Colombia. Con el ánimo de conocer cuáles son realmente los problemas con que se enfrentan los investigadores se realizó una revisión de las solicitudes de acceso a recursos genéticos y permisos de investigación que se tramitan ante el Ministerio de Ambiente, Vivienda y...

  2. Microsatélites, distancias genéticas y estructura de poblaciones nativas sudamericanas

    Directory of Open Access Journals (Sweden)

    Demarchi, Darío Alfredo

    2009-01-01

    Full Text Available En este trabajo se investigaron las relaciones genéticas entre 17 poblaciones nativas sudamericanas en relación a 15 microsatélites (STRs autosómicos, utilizando 3 distancias genéticas- DST, DAy (δu2-que se ajustan a diferentes postulados teóricos. A través de diferentes técnicas de análisis (escalamiento multidimensional, correlación y correlación parcial de matrices se puso a prueba si las distancias genéticas reflejaban las relaciones interpoblacionales esperadas a partir de la distribución geográfica o de relaciones lingüísticas entre las poblaciones. Además, se estimó en que grado las distintas medidas de distancias genéticas eran influenciadas por la diversidad (He de cada población. Los mapas genéticos muestran, principalmente para DST y DA, que las poblaciones aisladas y con bajo tamaño efectivo (Ne aparecen como outliers, mientras que las poblaciones con alto Ne y mayor flujo génico ocupan una posición central a bajos valores de distancia unas de otras y sin un patrón definido de agrupamiento. La falta de asociación entre distancias genéticas y lingüísticas o geográficas y por otra parte, la alta correlación negativa entre He y distancias génicas promedio por población confiman ese patrón, demostrando que la mayor parte de la variación interpoblacional puede ser explicada en función del grado de diversidad intrapoblacional. Es decir, las distancias genéticas no reflejan relaciones filogenéticas, lingüísticas o geográficas, sino más bien eventos demográficos recientes tales como cuellos de botella genético, efecto fundador o migración externa masiva. Este hecho puede ser comprobado por medio de otra metodología analítica, el modelo de Harpending y Ward.

  3. A psicologia face aos novos progressos da genética humana

    OpenAIRE

    Rossano André Dal-Farra; Emerson Juliano Prates

    2004-01-01

    Os avanços da Genética contemporânea têm suscitado uma grande polêmica a respeito da gênese do homem e das possibilidades de agir sobre a biologia humana, repercutindo intensamente sobre os mais diversos ramos do conhecimento. Considerando a importância do tema, este texto pretende apresentar de forma sucinta os principais resultados de pesquisas sobre as bases genéticas do comportamento humano, com o foco voltado para a importância do profissional de Psicologia em questões relativas ao assun...

  4. Variabilidad genética y gestión forestal

    OpenAIRE

    Alía, R.; D. Agúndez; Alba, N; S.C. González Martínez; A. Soto

    2003-01-01

    La diversidad biológica, su conservación e incremento, es uno de los principios básicos de la gestión forestal sostenible. La alta variabilidad genética de las especies forestales es responsable de los procesos de adaptación ante factores bióticos y abióticos extremos que, a su vez, aseguran la persistencia frente a los riesgos a los que están sometidas las masas forestales. En este trabajo se analizan distintos aspectos relacionados con la variabilidad genética de las especies fores...

  5. Detecció i estudi de gens implicats en el desenvolupament prenatal de la rata

    OpenAIRE

    2002-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Introducció El treball "Detecció i estudi de gens implicats en el desenvolupament prenatal de la rata" parteix de la hipòtesi de treball de què la carcinogènesi i el desenvolupament són processos que comparteixen mecanismes de funcionament com són l'elevat índex de proliferació, la capacitat de migració cel·lular o l'angiogènesi sostinguda i que, per tant, deuen compartir els gens que els regulen. Amb aquest plantejament...

  6. Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae), new nematode genera as parasites of Passalidae (Coleoptera) from the Democratic Republic of Congo.

    Science.gov (United States)

    Morffe, Jans; García, Nayla

    2013-01-01

    Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973) comb. n. and B. annulatum (Van Waerebeke, 1973) comb. n. Chokwenema lepidophorum gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema) that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic.

  7. Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae, new nematode genera as parasites of Passalidae (Coleoptera from the Democratic Republic of Congo

    Directory of Open Access Journals (Sweden)

    Jans Morffe

    2013-12-01

    Full Text Available Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized byfemales with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973 comb. n. and B. annulatum (Van Waerebeke, 1973 comb.n. Chokwenema lepidophorum gen. n. et sp. n. is characterized byfemales with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic.

  8. Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

    Science.gov (United States)

    Breider, Sven; Scheuner, Carmen; Schumann, Peter; Fiebig, Anne; Petersen, Jörn; Pradella, Silke; Klenk, Hans-Peter; Brinkhoff, Thorsten; Göker, Markus

    2014-01-01

    Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria) genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus, and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov., and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.

  9. Resistência genética à mancha-bacteriana em genótipos de pimentão

    OpenAIRE

    Costa Roberto Alexandre; Rodrigues Rosana; Sudré Cláudia Pombo

    2002-01-01

    A mancha-bacteriana, principal doença bacteriana do pimentão causa desfolha intensa quando em condições favoráveis, deixando os frutos expostos ao sol, depreciando-os e diminuindo a produção. Para estimar, nas condições de Campos dos Goytacazes, os efeitos genéticos da reação do hospedeiro ao patógeno, tanto em folhas como em frutos, foram obtidos híbridos F1, sem recíprocos, a partir de cruzamentos dialélicos entre cinco genótipos de pimentão, sendo três suscetíveis ('UENF 1420', 'UENF 1421'...

  10. Genetic diversity in Australian Cedar genotypes selected by mixed models Diversidade genética em genótipos de Cedro Australiano selecionados via modelos mistos

    Directory of Open Access Journals (Sweden)

    Rulfe Tavares

    2012-04-01

    Full Text Available The increasing demand for raw material for multiple uses of forest products and by-products has attracted the interest for fast growing species, such as the Australian Cedar (Toona ciliata, which presents high productive and economic potential. The present work aimed at estimating genetic diversity by DNA markers and morphological traits supported for the mixed models. The following traits were measured and genotypes were sampled randomly in different areas: diameter at breast height, height, cylindrical volume, diameter, distance between nodes and crown diameter. Twelve RAPD primers were used and generated a total of 91 marks, 82 of which were polymorphic. The high percentage of polymorphic markers, 90.10%, demonstrated that discrimination in this species is efficient, but it yet little studied, for this case we can find the extent of the genetic basis for the application of technical improvement. The assessment of genetic diversity by the UPGMA method using the binary and morphological data provided the expression of genetic dissimilarities among the accessions evaluated, optimizing the perception of this divergence. The use of mixed models was efficient to assess combined genetic diversity to optimize the selection of genotypes with divergent genetic values for diameter at breast height.A crescente demanda por matéria-prima para múltiplos usos dos produtos florestais e subprodutos tem despertado o interesse para espécies de crescimento rápido, como o cedro australiano (Toona ciliata, que apresenta potencial produtivo e econômico. Neste trabalho, objetivou-se estimar a diversidade genética por marcadores de DNA e caracteres morfológicos com o uso dos modelos mistos. Os seguintes caracteres foram medidos e os genótipos foram amostrados aleatoriamente em diferentes áreas de plantio: diâmetro à altura do peito, altura, volume cilíndrico, distância entre nós e diâmetro de copa. Doze primers RAPD foram utilizados e geraram um total

  11. História da genética no Brasil: um olhar a partir do Museu da Genética da Universidade Federal do Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Vanderlei Sebastiao de Souza

    2013-06-01

    Full Text Available Aborda o contexto de criação do Museu da Genética, em 2011 no Departamento de Genética na Universidade Federal do Rio Grande do Sul, em Porto Alegre, e apresenta sua estrutura e conteúdo. Argumenta-se que os materiais disponibilizados no Museu da Genética constituem uma rica fonte para pesquisas sobre a história da genética no Brasil (e da genética de populações humanas em particular a partir da segunda metade do século XX, tema ainda pouco investigado, apesar da proeminência dessa área do conhecimento no Brasil.

  12. 75 FR 1052 - Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of...

    Science.gov (United States)

    2010-01-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of Filing December 30, 2009. Take notice that on December 24, 2009, Terra-Gen...

  13. Discourses in Conflict: The Relationship between Gen Y Pre-Service Teachers, Digital Technologies and Lifelong Learning

    Science.gov (United States)

    Donnison, Sharn

    2009-01-01

    This paper analyses Gen Y pre-service primary school teachers' conceptualisations of lifelong learning. It is situated within a context of improving the provision and delivery of pre-service teacher education. This paper argues that Gen Y's understanding of lifelong learning has been influenced by their engagements with digital technologies and…

  14. Autosomal SNP typing of forensic samples with the GenPlex(TM) HID System: Results of a collaborative study

    DEFF Research Database (Denmark)

    Tomas, C.; Axler-DiPerte, G.; Budimlija, Z.M.

    2011-01-01

    The GenPlex(TM) HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex(TM) HID System using 250-500 pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in ...

  15. Microcenoscelis n. gen. (Coleoptera: Tenebrionidae: Ulomini) from tropical Africa, with description of a blind species from Zimbabwe.

    Science.gov (United States)

    Schawaller, Wolfgang

    2015-10-05

    Microcenoscelis n. gen. (Coleoptera: Tenebrionidae: Ulomini) caeca n. sp. is described from Zimbabwe, a small and completely blind species. A second known species, however with completely developed eyes, and originally described as Uloma minuscula Ardoin, 1969, was also placed in the new genus. Microcenoscelis n. gen. seems to be mostly related to the genera Cenoscelis Wollaston, 1867, and Cneocnemis Gebien, 1914.

  16. Zebra Delivers EPC Gen 2 RFID Printing/Encoding With Industry’s First Free,Downloadable Firmware Upgrade

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Companies can add Gen2 support on existing Zebra RFID printer/encoders while continuing to use Gen 1 and other protocols: no new hardware required. Vernon Hills,Ⅲ., December 14,2005-Zebra Technologies (Nasdaq:ZBRA),a leading manufacturer of radio frequency identification (RFID) printer/encoders worldwide, today

  17. Astioglossimetra karachiensis n. gen., n. sp. (Trematoda: Plagiorchiidae: Astiotrematinae) from the Marine Turtle Chelonia mydas of Karachi Coast

    OpenAIRE

    BILQEES, Fatima Mujib

    2002-01-01

    A new trematode genus Astioglossimetra n. gen. has been erected to accommodate an undescribed species A. karachiensis n. gen., n. sp., from Chelonia mydas of the Karachi coast. The genus name refers to its relation to the genera Astiotrema Looss and Glossimetra Mehra of the family Plagiorchiidae and subfamily Astiotrematinae.

  18. Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.

    Science.gov (United States)

    Nedashkovskaya, Olga I; Kim, Seung Bum; Lysenko, Anatoly M; Frolova, Galina M; Mikhailov, Valery V; Lee, Kang Hyun; Bae, Kyung Sook

    2005-01-01

    The taxonomic position of three novel sea-water isolates was determined. The strains studied were strictly aerobic, heterotrophic, pigmented, motile by gliding, Gram-negative and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. 16S rRNA gene sequence phylogenetic analysis indicated that the strains KMM 6020T, KMM 6021 and KMM 6028 occupied a distinct lineage within the family Flavobacteriaceae. The major respiratory quinone was MK-6. The predominant fatty acids were i15 : 0, i15 : 1, i15 : 0 3-OH, i17 : 1omega9c and i17 : 0 3-OH. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacteria were assigned to the genus Aquimarina gen. nov., as Aquimarina muelleri gen. nov., sp. nov. The type strain is KMM 6020T (=KCTC 12285T=LMG 22569T). From the results of the 16S rRNA gene sequence analysis and phenotypic features, the species [Cytophaga] latercula Lewin 1969 is proposed to be reclassified in the new genus Stanierella as Stanierella latercula gen. nov., comb. nov., with type strain CIP 104806T (=ATCC 23177T=NCIMB 1399T=LMG 1343T).

  19. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia. Bases genéticas de la esquizofrenia: "Nurture vrs Nature

    Directory of Open Access Journals (Sweden)

    Henriette Raventós-Vorst

    2003-01-01

    Full Text Available El presente artículo revisa la evidencia científica que muestra la heredabilidad de la esquizofrenia, su forma de herencia compleja y la posible heterogeneidad genética y ambiental. Se presentan las regiones cromosómicas que han sido ligadas a la enfermedad y algunos de los genes candidatos. El objetivo es presentar los resultados más importantes en el campo de la investigación genética de la enfermedad. Aunque se acepta que factores ambientales deben estar presentes en la etiopatogenia de la enfermedad, no se profundiza en ellos. Finalmente, se comenta el modelo lamarquiano sugerido por el Prof.. Bolaños. El fin es transmitir que en la actualidad no hay contradicción entre el modelo biologista o psicológico que explicaban esta enfermedad. La concepción moderna une ambos modelos: se considera una enfermedad del neurodesarrollo en la que participan factores genéticos, factores epigenéticos y noxas ambientales, incluyendo los factores psicosociales.

  20. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia. Esquizofrenia, genética y complejidad

    Directory of Open Access Journals (Sweden)

    Henning Jensen Pennington

    2003-01-01

    Full Text Available El artículo plantea la necesidad de no sobresimplificar fenómenos complejos como el de la esquizofrenia, pues su etiología implica la interacción de aspectos genéticos, biológicos y sociopsicológicos. Se plantea que el mismo conocimiento genético imposibilita la asunción de estas posiciones radicales, razón por la cual la investigación biológica actúa con cautela en la formulación de hipótesis causales. No obstante, se pone de manifiesto la interpretación inapropiada de los resultados de investigaciones genéticas y biológicas, cuando por un lado, se postula la complejidad del fenómeno, pero por otro lado y por un acto de simplificación cognoscitiva, se desatiende esa complejidad y se da el salto hacia explicaciones monocausales. La actividad investigativa en el campo de la esquizofrenia se enfrenta entonces a la responsabilidad histórica de dar a cada elemento el lugar que le corresponde en la intrincada red de elementos que influyen en la aparición y desarrollo de la esquizofrenia.

  1. Chitinozoan faunas from the Rügen Ordovician (Rügen 5/66 and Binz 1/73 wells), NE Germany.

    Science.gov (United States)

    Samuelsson, J; Verniers, J; Vecoli, M

    2000-12-01

    The island of Rügen (NE Germany), situated close to the Trans-European Suture Zone (TESZ), in the southern Baltic Sea is underlain by sedimentary rocks of an Early Palaeozoic age, known only from boreholes. The wells, Rügen 5/66 and Binz 1/73, were investigated for their chitinozoan assemblages to improve the earlier biostratigraphic dating (graptolites and acritarchs) and to facilitate comparisons with other chitinozoan assemblages on both sides of the TESZ. In the lower part of the Rügen 5/66 core (3794.7-3615.8m), Lagenochitina destombesi Elaouad-Debbaj is indicative of an early late Tremadoc age. In the upper part of the same well (3287.3-1709.7m), the observed chitinozoan taxa suggest an age spanning the early Llanvirn to the Caradoc. The entire sampled interval of the Binz 1/73 core (5217.6-5041.8m) is interpreted as belonging to the Siphonochitina formosa Biozone (early-early late Abereiddian, corresponding to the early Llanvirn). The chitinozoan data corroborate the earlier suggested biostratigraphic ages, based on acritarchs and graptolites. The chitinozoans from the Binz 1/73 well point to a high latitude provenance of the investigated host sediments at time of deposition.

  2. O impacto da genética na asma infantil Impact of genetics in childhood asthma

    Directory of Open Access Journals (Sweden)

    Leonardo A. Pinto

    2008-08-01

    Full Text Available OBJETIVO: Apresentar os resultados dos estudos mais importantes e recentes sobre a genética da asma. Estes dados devem auxiliar os clínicos gerais a compreender o impacto da genética sobre este distúrbio complexo e como os genes e polimorfismos influenciam a asma e a atopia. FONTES DOS DADOS: Os dados foram coletados do banco de dados MEDLINE. Os estudos de associação genética foram selecionados do Genetic Association Database, um repositório de estudos de associação genética de doenças e distúrbios complexos organizado pelo National Institutes of Health. SÍNTESE DOS DADOS: Considerando os dados de diversos importantes estudos de gêmeos sobre a genética da asma, a heritabilidade, que mensura a contribuição dos fatores genéticos para a variância da asma, pode ser estimada entre 0,48 e 0,79. Uma grande quantidade de estudos de associação genética tentou identificar genes de susceptibilidade à asma. Os resultados mais replicados nos estudos de associação genética envolvem as cinco regiões do genoma humano a seguir: 5q31-32, 6p21, 11q12-13, 16p11-12, e 20p13. Recentemente, outro gene de susceptibilidade à asma (ORMDL3, considerado determinante crítico para a asma infantil, foi identificado por um estudo genômico de associação. CONCLUSÕES: É possível estimar que a contribuição genética à asma varia entre 48 e 79%. Diversos loci parecem influenciar a susceptibilidade à asma. Os genes localizados no cromossomo 5q (ADRB2, IL13 e IL4 e o gene ORMDL3, no cromossomo 17, identificado recentemente, parecem ser determinantes para a asma infantil. O diagnóstico e a farmacogenética podem ser as primeiras implicações clínicas de estudos extensivos sobre a genética da asma.OBJECTIVE: To present the most important and recent results of studies on asthma genetics. These data may help general physicians understand the impact of genetics on this complex disorder and how genes and polymorphisms influence asthma and atopy

  3. Genetic similarity between coriander genotypes using ISSR markers Similaridade genética entre genótipos de coentro por marcadores ISSR

    Directory of Open Access Journals (Sweden)

    Roberto de A Melo

    2011-12-01

    Full Text Available With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16, resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.Com o surgimento de novas cultivares, uma caracterização genética precisa é essencial, visando à utilização em programas de melhoramento ou para fins de registros e ou proteção de cultivares. Marcadores moleculares vêm complementando a caracterização morfológica e agronômica tradicional, uma vez que são virtualmente ilimitados, cobrem todo o genoma e não são influenciados pelo ambiente. A caracterização genética constitui a base para

  4. Variabilidad genética de la respuesta inflamatoria: I. Polimorfismo -511 C/T en el gen IL1β en diferentes subpoblaciones peruanas

    Directory of Open Access Journals (Sweden)

    Óscar Acosta

    2012-07-01

    Full Text Available El polimorfismo -511 citosina/timina (-511 C/T en la región promotora del gen interleuquina 1 beta (IL1β estα implicado en la producciσn diferencial de la citoquina y por tanto puede estar asociado a la respuesta inmuno-inflamatoria en obesidad, dislipidemias, cardiopatías, cáncer, infecciones, y el tratamiento con nutrientes y fármacos. Objetivos: Establecer la distribución de frecuencias de los genotipos y alelos del polimorfismo -511 C/T del gen IL1β en diferentes subpoblaciones peruanas. Diseño: Estudio descriptivo, observacional, transversal. Instituciones: Centro de Investigación de Bioquímica y Nutrición e Instituto de Medicina Tropical D.A. Carrión, Facultad de Medicina, UNMSM y Centro de Genética y Biología Molecular, Facultad de Medicina, USMP, Lima, Perú. Participantes: Pobladores peruanos. Intervenciones: Extracción de ADN genómico a partir de muestras sanguíneas o epitelio bucal según metodología estándar, de 168 individuos de 9 grupos subpoblacionales: 23 mestizos de Lima, 33 amazónicos (20 de Pucallpa y 13 de Amazonas y 112 andinos (12 de Ancash, 10 de Cajamarca, 18 de Huarochirí-Lima, 25 de Puno-Taquile, 25 de Puno-Uros y 22 de Puno-Anapia. Análisis del polimorfismo -511 C/T mediante la técnica de PCR/RFLP, con primers específicos y digestión con la enzima de restricción AvaI, detectándose los fragmentos por electroforesis en geles de agarosa al 2% y tinción con bromuro de etidio. Principales medidas de resultados: Frecuencias genotípicas y alélicas del gen IL1β. Resultados: Se encontró las siguientes frecuencias genotípicas CC=0,024; CT=0,369 y TT=0,607, consistentes con el equilibrio de Hardy-Weinberg; y las frecuencias alélicas fueron alelo C=0,208 y aleloT= 0,792. La frecuencia del alelo T, considerado el mutante, fue muy alta en los Uros de Puno (0.940 y más baja en los mestizos de Lima (0.609. La comparación de las frecuencias genotípicas (TT versus CT+CC y alélicas (T versus C

  5. Targeted amplicon sequencing (TAS): a scalable next-gen approach to multilocus, multitaxa phylogenetics.

    Science.gov (United States)

    Bybee, Seth M; Bracken-Grissom, Heather; Haynes, Benjamin D; Hermansen, Russell A; Byers, Robert L; Clement, Mark J; Udall, Joshua A; Wilcox, Edward R; Crandall, Keith A

    2011-01-01

    Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples. Our TAS approach is easily scalable, simple in execution, neither time-nor labor-intensive, relatively inexpensive, and can be applied to a broad diversity of organisms and/or genes. Our TAS approach includes a bioinformatic application, BarcodeCrucher, to take raw next-gen sequence reads and perform quality control checks and convert the data into FASTA format organized by gene and sample, ready for phylogenetic analyses. We demonstrate our approach by sequencing targeted genes of known phylogenetic utility to estimate a phylogeny for the Pancrustacea. We generated data from 44 taxa using 68 different 10-bp multiplexing identifiers. The overall quality of data produced was robust and was informative for phylogeny estimation. The potential for this method to produce copious amounts of data from a single 454 plate (e.g., 325 taxa for 24 loci) significantly reduces sequencing expenses incurred from traditional Sanger sequencing. We further discuss the advantages and disadvantages of this method, while offering suggestions to enhance the approach.

  6. The political thought of Madame de Genlis: Rousseau’s Royalist Legacy

    Directory of Open Access Journals (Sweden)

    Carolina Armenteros

    2013-09-01

    Full Text Available On sait très peu sur la pensée politique de Félicité de Genlis (1746-1830 à part le fait qu’elle est devenue royaliste après la Révolution française. Cependant le tableau de l’Arcadie de Lagaraye dans Adèle et Théodore, ou lettres sur l’éducation (1782, fournit beaucoup de détails sur ce sujet. La figure du Législateur, en particulier, y démontre que la pensée politique de Genlis s’intéresse principalement à l’administration émotive des sociétés politiques, surtout en ce qui regarde les femmes, tout en rappelant – et en critiquant – les préoccupations politiques de Jean-Jacques Rousseau. Cet article analyse les correspondances et les contrastes entre Lagaraye et l’Arcadie de Clarens que Rousseau a dépeint dans Julie, ou la nouvelle Héloïse (1762. Il met Genlis en avant comme une critique sévère des idées de Rousseau, ainsi qu’une adhérente secrète du rousseauisme politique qui a développé sa vision politique sur la base de celle du Citoyen de Genève.

  7. Molecular evolution of two lineages related to influenza B virus based on HA1 gen

    Institute of Scientific and Technical Information of China (English)

    金青青

    2013-01-01

    Objective To study the evolutionary characteristics and rules of two lineages on influenza B virus.Methods A total of 126 HA1 sequences of strains isolated during 1940 to 201 2were downloaded from the GenBank.Time of the most recent common

  8. [Morphological and histological studies on the Chinese drug shan-dou-gen].

    Science.gov (United States)

    Fang, L Q; Guo, J X

    1992-01-01

    The Chinese drug "Shan-Dou-Gen" has been used for removing toxic heat, promoting the subsidence of swelling and soothing the sore throat since the ancient time. The authors made a survey of the drug "Shan-Dou-Gen" available in drug markets as well as the plant origin from the drug producing districts. The results showed that the drug "Shan-Dou-Gen" used in different regions in China at present are the roots or rhizomes derived from 9 species: Sophora tonkinensis Gagnep. (Leguminosae), Menispermum dauricum DC. (Menispermaceae), Indigofera amblyantha Craib (Leguminosae), I. carlesii Carib, I., fortunei Craib, I. decoa Lindl. var. ichangensis Y. Y. Fang et C. Z. Zheng, I. kirilowii Maxim. et Palibin, I. potaninii Craib, and Beesia calthaefolia (Maxim.) Ulbr. (Ranuculaceae). In this paper, the morphological characters of the crude drugs are described, compared and illustrated with photographs. The histological structures of the used parts are described, compared and illustrated with line drawings. The morphological and histological similarities and differences found among the above 9 species are summarized, and the key for the identification of the crude drugs is provided. As the drug "Shan-Dou-Gen" derived from different species has different actions and dosages, it is necessary to give different names to different species and use them correctly.

  9. Gen Y Recruitment: Understanding Graduate Intentions to Join an Organisation Using the Theory of Planned Behaviour

    Science.gov (United States)

    Warmerdam, Amanda; Lewis, Ioni; Banks, Tamara

    2015-01-01

    Purpose: Using the Theory of Planned Behaviour (TPB) framework, the purpose of this paper is to explore whether the standard TPB constructs explained variance in Generation Y (Gen Y) individuals' intentions to join their ideal organisation. Design/methodology/approach: A mixed methods approach was used featuring qualitative and quantitative…

  10. A genética das epilepsias The genetics of epilepsies

    National Research Council Canada - National Science Library

    Iscia Lopes-Cendes

    2008-01-01

    .... Mais recentemente, estudos de genética molecular e estratégias de descoberta de genes foram usados para revelar os mecanismos moleculares e celulares envolvidas em diversas síndromes epilépticas mendelianas. SÍNTESE DOS DADOS...

  11. Pacaella massiliensis gen. nov., sp. nov., a new bacterial species isolated from the human gut

    Directory of Open Access Journals (Sweden)

    S. Ndongo

    2017-03-01

    Full Text Available Herein, we report the main characteristics of a new species named Pacaella massiliensis gen. nov., sp. nov., strain Marseille-P2670T (CSUR P2670 that was isolated from the gut microbiota of a 45-year-old French patient.

  12. Au-delà du moralisme : Madame de Genlis et l’hospitalité

    Directory of Open Access Journals (Sweden)

    Sophie Bourgault

    2013-09-01

    Full Text Available Très peu de philosophes politiques se sont intéressés à l’oeuvre de Genlis, en partie parce que son oeuvre est communément réduite à un moralisme religieux insipide. Si Alice Laborde con-clut sa biographie en soulignant que Genlis « est moraliste avant tout », les travaux récents de Mary Trouille mettent tout autant l’accent sur le fait que la comtesse est « moralist to the core ». La visée du présent article est de nuancer cette lecture, non pas en niant le moralisme de Genlis, mais plutôt en montrant que celui-ci ne l’a pas empêchée de publier des textes à portée politique qui mériteraient notre attention aujourd’hui—particulièrement l’attention de ceux et celles qui se donnent pour tâche de penser l’accueil et le soin de l’Autre. A partir du Discours sur la sup-pression des convens et du Discours sur le luxe et l’hospitalité, nous indiquons non seulement l’actualité, mais aussi la surprenante radicalité des textes publiés par Genlis en période révolu-tionnaire.

  13. GWAS in a box: statistical and visual analytics of structured associations via GenAMap.

    Science.gov (United States)

    Xing, Eric P; Curtis, Ross E; Schoenherr, Georg; Lee, Seunghak; Yin, Junming; Puniyani, Kriti; Wu, Wei; Kinnaird, Peter

    2014-01-01

    With the continuous improvement in genotyping and molecular phenotyping technology and the decreasing typing cost, it is expected that in a few years, more and more clinical studies of complex diseases will recruit thousands of individuals for pan-omic genetic association analyses. Hence, there is a great need for algorithms and software tools that could scale up to the whole omic level, integrate different omic data, leverage rich structure information, and be easily accessible to non-technical users. We present GenAMap, an interactive analytics software platform that 1) automates the execution of principled machine learning methods that detect genome- and phenome-wide associations among genotypes, gene expression data, and clinical or other macroscopic traits, and 2) provides new visualization tools specifically designed to aid in the exploration of association mapping results. Algorithmically, GenAMap is based on a new paradigm for GWAS and PheWAS analysis, termed structured association mapping, which leverages various structures in the omic data. We demonstrate the function of GenAMap via a case study of the Brem and Kruglyak yeast dataset, and then apply it on a comprehensive eQTL analysis of the NIH heterogeneous stock mice dataset and report some interesting findings. GenAMap is available from http://sailing.cs.cmu.edu/genamap.

  14. Bornean freshwater crabs of the genus Arachnothelphusa gen. nov. (Crustacea: Decapoda: Brachyura: Gecarcinucidae)

    NARCIS (Netherlands)

    Ng, P.K.L.

    1991-01-01

    A new genus, Arachnothelphusa gen. nov., is established for five species of Bornean freshwater crabs (Gecarcinucidae) previously placed in Thelphusula Bott, 1969. One species from Sabah, A. terrapes, is here described as new. The genus is characterised by its long ambulatory legs, shape of the carap

  15. Qasimia gen. nov., an early Marattia-like fern from the Permian of Saudi Arabia

    NARCIS (Netherlands)

    Hill, C.R.; Wagner, R.H.; El-Khayal, A. A.

    1985-01-01

    The marattialean fern Qasimia schyfsmae (Lemoigne) gen. et comb. nov. is described from the Late Permian plant bed at Unayzah in central Saudi Arabia. Although no organic matter is preserved, impregnation of the compressions by iron minerals at an early stage of diagenesis has partly mineralised the

  16. Working with Gen Y Teachers: Dealing with a Changing Teacher Workforce

    Science.gov (United States)

    Williamson, Ronald; Meyer-Looze, Catherine

    2010-01-01

    A significant change is coming to American classrooms. As Baby Boomer teachers retire and are replaced by members of Generation Y (born between 1977 and 1995) there is growing evidence that they come from a far different set of experiences, experiences that will shape their lives and their careers (Coggins, 2008). Members of Gen Y share several…

  17. ‘Woes en Leeg’ en ‘Duisternis’ in Gen. 1 (1

    Directory of Open Access Journals (Sweden)

    J. L. Helberg

    1967-06-01

    Full Text Available Die Ieser word vir besonderhede en vir die verskillende beskouinge oor Gen. I verwys na die werke van Du Toit, Kroeze, N. H. Ridderbos, Scheepers en (die artikel van E. J. Young, met hulle literatuurlyste, synde enkele van die nuutste werke oor die onderhawige saak.

  18. Revision of "Phyllobrotica" from Taiwan with description of Jolibrotica gen. n. (Coleoptera, Chrysomelidae, Galerucinae).

    Science.gov (United States)

    Lee, Chi-Feng; Bezděk, Jan

    2015-01-01

    All Taiwanese species formerly classified the genus Phyllobrotica Chevrolat, 1836 are revised. Jolibrotica Lee & Bezděk, gen. n., is described for Phyllobrotica sauteri (Chûjô, 1935) (Taiwan, China: Guangxi) and Phyllobrotica chujoi Kimoto, 1969 (Taiwan). Phyllobrotica shirozui Kimoto, 1969 is transferred to the genus Haplosomoides. All species are redescribed and their diagnostic characters illustrated.

  19. Characterization and design of the FutureGen 2.0 carbon storage site

    Energy Technology Data Exchange (ETDEWEB)

    Gilmore, Tyler; Bonneville, Alain; Sullivan, Charlotte; Kelley, Mark; Appriou, Delphine; Vermeul, Vince; White, Signe; Zhang, Fred; Bjornstad, Bruce; Cornet, Francois; Gerst, Jacqueline; Gupta, Neeraj; Hund, Gretchen; Horner, Jake; Last, George; Lanigan, Dave; Oostrom, Mart; McNeil, Caitlin; Moody, Mark; Rockhold, Mark; Elliott, Mike; Spane, Frank; Strickland, Chris; Swartz, Lucy; Thorne, Paul; Brown, Christopher; Hoffmann, Jeffrey; Humphreys, Kenneth

    2016-10-01

    The objective of the FutureGen 2.0 Project was to demonstrate, at the commercial scale, the technical feasibility of implementing carbon capture and storage (CCS) in a deep saline formation in Illinois, USA. Over approximately 5 years, the FutureGen Alliance conducted a detailed site-selection process and identified a site for carbon sequestration storage in Morgan County, Illinois. The storage site was fully characterized, including the collection of seismic data and the drilling and characterization of a stratigraphic borehole. The characterization data provided critical input for developing a site-specific conceptual model and subsequent numerical modeling simulations. The modeling simulations, coupled with the upstream designs of the pipeline and power plant supported the development of a detailed 90 percent design that included the injection wells and associated control and monitoring infrastructure. Collectively, all these data were used by the FutureGen Alliance to develop the required documentation to support the applications for four underground injection control (UIC) permits (one for each proposed well). In August 2014, the U.S. Environmental Protection Agency issued four, first-of-their-kind, Class VI UIC permits for carbon sequestration in the United States to the FutureGen Alliance. The information and data generated under this project have been made publically available through reports and publications, including this journal and others.

  20. GWAS in a box: statistical and visual analytics of structured associations via GenAMap.

    Directory of Open Access Journals (Sweden)

    Eric P Xing

    Full Text Available With the continuous improvement in genotyping and molecular phenotyping technology and the decreasing typing cost, it is expected that in a few years, more and more clinical studies of complex diseases will recruit thousands of individuals for pan-omic genetic association analyses. Hence, there is a great need for algorithms and software tools that could scale up to the whole omic level, integrate different omic data, leverage rich structure information, and be easily accessible to non-technical users. We present GenAMap, an interactive analytics software platform that 1 automates the execution of principled machine learning methods that detect genome- and phenome-wide associations among genotypes, gene expression data, and clinical or other macroscopic traits, and 2 provides new visualization tools specifically designed to aid in the exploration of association mapping results. Algorithmically, GenAMap is based on a new paradigm for GWAS and PheWAS analysis, termed structured association mapping, which leverages various structures in the omic data. We demonstrate the function of GenAMap via a case study of the Brem and Kruglyak yeast dataset, and then apply it on a comprehensive eQTL analysis of the NIH heterogeneous stock mice dataset and report some interesting findings. GenAMap is available from http://sailing.cs.cmu.edu/genamap.

  1. Visualizing and Sharing Results in Bioinformatics Projects: GBrowse and GenBank Exports

    Science.gov (United States)

    Effective tools for presenting and sharing data are necessary for collaborative projects, typical for bioinformatics. In order to facilitate sharing our data with other genomics, molecular biology, and bioinformatics researchers, we have developed software to export our data to GenBank and combined ...

  2. Acantholachesilla gen. n. (Psocodea:'Psocoptera': Lachesillidae: Eolachesillinae: Graphocaeciliini) from Valle del Cauca, Colombia.

    Science.gov (United States)

    Aldrete, Alfonso N García; Manchola, Oscar Fernando Saenz; Obando, Ranulfo González

    2014-06-25

    Acantholachesilla saltoensis gen. et sp. n. is described from Valle del Cauca, Colombia, in the Lachesillidae, tribe Graphocaeciliini (Eolachesillinae). The genus is related to Dagualachesilla and Dagualachesilloides that occur in the same area, differing from them in the male clunial projection, phallosome, and female subgenital plate and gonapophyses.

  3. Riesgos sobre la salud de los alimentos modificados genéticamente: una revision bibliografica

    Directory of Open Access Journals (Sweden)

    José L. Domingo Roig

    2000-01-01

    Full Text Available A lo largo de 1999, se ha venido intensificando el debate sobre la seguridad de los alimentos modificados genéticamente, una importante y compleja área de investigación científica, la cual demanda unos estándares rigurosos. Diversos grupos, incluyendo asociaciones de consumidores y Organizaciones no Gubernamentales (ONGs han sugerido que todos los alimentos modificados genéticamente deberían ser sometidos a estudios a largo plazo con animales antes de su aprobación para el consumo humano. El principal objetivo de la presente revisión ha sido conocer cual es el estado actual de la cuestión en lo referente a los potenciales efectos adversos sobre la salud de los alimentos modificados genéticamente. Dos bases de datos, Medline y Toxline, así como una serie de direcciones de internet, han sido empleadas para la obtención de bibliografía. Aunque son numerosos los comentarios, noticias generales y cartas al Editor aparecidos en prestigiosas revistas, los artículos referentes a estudios experimentales sobre la seguridad de los alimentos modificados genéticamente son, sorprendentemente, muy escasos. Si se han obtenido resultados procedentes de la evaluación toxicológica de estos alimentos, no han sido publicados en revistas científicas y, por lo tanto, no han podido ser debidamente juzgados o contrastados.

  4. Pteroxena papillifera n. gen., n. sp., an endoparasitic organism (Copepoda?) from the gymnosomatous pteropod, Notobranchaea

    NARCIS (Netherlands)

    Stock, J.H.; Spoel, van der S.

    1976-01-01

    A single specimen of a strongly transformed, endoparasitic organism was found on the gymnosomatous pteropod Notobranchaea macdonaldi Pelseneer morpha pelseneeri Pruvot-Fol off Delaware Bay in the north-western Atlantic Basin. The parasite is described as Pteroxena papillifera n. gen., n. sp., and is

  5. GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism.

    Science.gov (United States)

    Chan, Ying Wai; West, Stephen

    2015-12-15

    Holliday junctions (HJs) that physically link sister chromatids or homologous chromosomes are formed as intermediates during DNA repair by homologous recombination. Persistent recombination intermediates are acted upon by structure-selective endonucleases that are required for proper chromosome segregation at mitosis. Here, we have purified full-length human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is analogous to that exhibited by the prototypic HJ resolvase E. coli RuvC. We find that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordinated incisions that lead to the formation of ligatable nicked duplex products. As observed with RuvC, cleavage of the first strand is rate limiting, while second strand cleavage is rapid. In contrast to RuvC, however, GEN1 is largely monomeric in solution, but dimerizes on the HJ. Using HJs containing non-cleavable phosphorothioate-containing linkages in one strand, we show that the two incisions can be uncoupled and that the first nick occurs upon GEN1 dimerization at the junction. These results indicate that the mechanism of HJ resolution is largely conserved from bacteria to man, despite a lack of sequence homology between the resolvases. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  6. Bases genéticas de la formación de fisuras labiales

    OpenAIRE

    Escobar, Lina María; Prada-Arismendy, Jeanette; Téllez, Carolina; Castellanos, Jaime

    2013-01-01

    El objetivo de este artículo, es revisar la información actualizada sobre la genética de la hendidura labio palatina y los genes reportados que pueden contribuir al desarrollo de esta compleja patología.

  7. Leucemia linfoblástica aguda infantil: una aproximación genómica

    Directory of Open Access Journals (Sweden)

    Silvia Jiménez-Morales

    2017-01-01

    En esta revisión se proporciona una visión global de la genómica de la LLA, describiendo algunas estrategias que contribuyen a la identificación de biomarcadores con potencial utilidad en la práctica clínica.

  8. Saying Hello World with GrGen.NET - A Solution to the TTC 2011 Instructive Case

    Directory of Open Access Journals (Sweden)

    Sebastian Buchwald

    2011-11-01

    Full Text Available We introduce the graph transformation tool GrGen.NET (www.grgen.net by solving the Hello World Case of the Transformation Tool Contest 2011 which consists of a collection of small transformation tasks; for each task a section is given explaining our implementation.

  9. A critical appraisal of ATLAS9 and NextGen 5 model atmospheres

    CERN Document Server

    Bertone, E; Chavez, M; Rodríguez, L H

    2001-01-01

    The fitting atmosphere parameters (Teff, g, and [Fe/H]) for over 300 stars in the Gunn & Striker and Jacoby et al. catalogs have been obtained relying on the Kurucz (1992) ATLAS9 and Hauschildt et al (1999) NextGen5 synthesis models. The output results are compared, and a critical appraisal of both theoretical codes is performed.

  10. When Opportunity Only Knocks Once: Lessons From Gen Petraeus In Iraq And Afghanistan

    Science.gov (United States)

    2016-03-01

    GEN McChrystal as well as Petraeus for their flattening this special operations organization and making it more lethal across multiple theaters.17...U.S. face and a necessary unity of effort for from the U.S. to help recover Iraq from its civil war. This teamwork was especially helpful to reduce

  11. pGenN, a gene normalization tool for plant genes and proteins in scientific literature.

    Directory of Open Access Journals (Sweden)

    Ruoyao Ding

    Full Text Available Automatically detecting gene/protein names in the literature and connecting them to databases records, also known as gene normalization, provides a means to structure the information buried in free-text literature. Gene normalization is critical for improving the coverage of annotation in the databases, and is an essential component of many text mining systems and database curation pipelines.In this manuscript, we describe a gene normalization system specifically tailored for plant species, called pGenN (pivot-based Gene Normalization. The system consists of three steps: dictionary-based gene mention detection, species assignment, and intra species normalization. We have developed new heuristics to improve each of these phases.We evaluated the performance of pGenN on an in-house expertly annotated corpus consisting of 104 plant relevant abstracts. Our system achieved an F-value of 88.9% (Precision 90.9% and Recall 87.2% on this corpus, outperforming state-of-art systems presented in BioCreative III. We have processed over 440,000 plant-related Medline abstracts using pGenN. The gene normalization results are stored in a local database for direct query from the pGenN web interface (proteininformationresource.org/pgenn/. The annotated literature corpus is also publicly available through the PIR text mining portal (proteininformationresource.org/iprolink/.

  12. Macrostylopyga gen. nov., a new genus of cockroaches (Dictyoptera: Blattidae), with descriptions of two new species.

    Science.gov (United States)

    Anisyutkin, Leonid N; Anichkin, Alexandr E; Thinh, Nguyen Van

    2013-01-01

    The genus Macrostylopyga gen. nov. and two species (M. grandis sp. nov. and M. bidupi sp. nov.) are described. A detailed morphological description with special attention to the male genitalic structures is provided. Some aspects of the evolution of wingless cockroaches are briefly discussed.

  13. Gen Y Recruitment: Understanding Graduate Intentions to Join an Organisation Using the Theory of Planned Behaviour

    Science.gov (United States)

    Warmerdam, Amanda; Lewis, Ioni; Banks, Tamara

    2015-01-01

    Purpose: Using the Theory of Planned Behaviour (TPB) framework, the purpose of this paper is to explore whether the standard TPB constructs explained variance in Generation Y (Gen Y) individuals' intentions to join their ideal organisation. Design/methodology/approach: A mixed methods approach was used featuring qualitative and quantitative…

  14. Karksilepis parva gen. et sp. nov. (Chondrichthyes from the Burtnieki Regional Stage, Middle Devonian of Estonia

    Directory of Open Access Journals (Sweden)

    Kleesment, Anne

    2008-12-01

    Full Text Available Dermal scales of a new chondrichthyan were discovered in four levels in sandstones of the Härma Beds (lower part of the Burtnieki Regional Stage, Middle Devonian in the Karksi outcrop of South Estonia. The classification of Karksilepis parva gen. et sp. nov. Märss is still uncertain.

  15. Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA

    Directory of Open Access Journals (Sweden)

    Yijin Liu

    2015-12-01

    Full Text Available We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction.

  16. Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.

    Science.gov (United States)

    Reinhold-Hurek, B; Hurek, T

    2000-03-01

    The taxonomic structure of members of the genus Azoarcus sensu lato was reassessed in a polyphasic approach. Two species, Azoarcus communis and Azoarcus indigens, three unnamed species containing diazotrophs associated with Kallar grass roots (groups C, D) and a group of strains (E) isolated from fungi were analysed. They were compared by PAGE analyses of cellular proteins, genomic fingerprints, morphological and nutritional features to new isolates from rice roots. All strains within groups C, D and E containing 5-12 isolates showed group-specific cell and colony morphology and carbon source utilization patterns, with exception of the obligately microaerobic strain BS20-3, a member of group C. All strains, with this exception, also had almost indistinguishable electrophoretic protein patterns and genomic fingerprints generated with tDNA-directed primers, suggesting they belong to the same species. Phylogenetic analyses of almost complete 16S rDNA sequences carried out with three different algorithms (neighbour-joining, maximum-likelihood, parsimony) revealed that Azoarcus sensu lato is not monophyletic. Groups C, D and E formed three distinct lineages located between the Azoarcus/Thauera and the Rhodocyclus clusters. Phylogenetic distances between groups C, D and E were as large as between other genera (93-94% sequence similarity). This suggested they have the rank of three different genera. Since it was possible to differentiate them from each other and other related bacteria by phenotypic features, three new genera with one type species each are proposed: Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.

  17. Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.

    Science.gov (United States)

    Hamada, Moriyuki; Iino, Takao; Iwami, Takahiro; Harayama, Shigeaki; Tamura, Tomohiko; Suzuki, Ken-ichiro

    2010-01-01

    Two Gram-positive bacteria, designated strains Aji5-31(T) and Ngc37-23(T), were isolated from the intestinal tracts of fishes. 16S rRNA gene sequence analysis indicated that both strains were related to the members of the family Dermatophilaceae, with 95.6-96.9% 16S rRNA gene sequence similarities. The family Dermatophilaceae contains 2 genera and 3 species: Dermatophilus congolensis, Dermatophilus chelonae and Kineosphaera limosa. However, it has been suggested that the taxonomic position of D. chelonae should be reinvestigated using a polyphasic approach, because the chemotaxonomic characteristics are not known (Stackebrandt, 2006; Stackebrandt and Schumann, 2000). Our present study revealed that strains Aji5-31(T), Ngc37-23(T) and D. chelonae NBRC 105200(T) should be separated from the other members of the family Dermatophilaceae on the basis of the following characteristics: the predominant menaquinone of strain Aji5-31(T) is MK-8(H(2)), strain Ngc37-23(T) possesses iso- branched fatty acids as major components, and the menaquinone composition of D. chelonae is MK-8(H(4)), MK-8 and MK-8(H(2)) (5 : 3 : 2, respectively). On the basis of these distinctive phenotypic characteristics and phylogenetic analysis results, it is proposed that strains Aji5-31(T) and Ngc37-23(T) be classified as two novel genera and species of the family Dermatophilaceae. The names are Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., and the type strains are Aji5-31(T) (=NBRC 104925(T) =DSM 22762(T)) and Ngc37-23(T) (=NBRC 104926(T) =DSM 22761(T)), respectively. In addition, D. chelonae should be reassigned to a new genus of the family Dermatophilaceae with the name Austwickia chelonae gen. nov., comb. nov.

  18. Diversidad genética en poblaciones humanas de dos regiones colombianas

    Directory of Open Access Journals (Sweden)

    Fernando Rondón

    2008-06-01

    Full Text Available Introducción: Estudios preliminares han mostrado la existencia de relaciones genéticas entre las poblaciones humanas del sur-occidente y las de la región andina colombiana, teniendo esto implicaciones en el grado de miscegenación de estas comunidades. No obstante el reconocimiento de este amplio proceso de mestizaje, no se tiene suficiente información que permita establecer la estructura y el grado de diversidad genética para cada región en particular y de la población colombiana en general. Objetivo: Determinar la estructura y diversidad genética presente en grupos poblacionales del centro y sur-occidente colombiano.Metodología: Se analizaron las frecuencias alélicas de 12 sistemas de microsatélites autosómicos y el tipo y frecuencia de RFLP’s de mtDNA presentes en 472 individuos de tres grupos étnicos: mestizos, indígenas y afroamericanos.Resultados: La caracterización de haplotipos de mtDNA en individuos afrodescendientes presentó 15% de marcadores típicos amerindios y 43% de africanos. El análisis de la diversidad genética mostró un índice de 0.72 en individuos Pijaos, valor cercano al índice de diversidad de la población mestiza de Cali (0.75. El análisis molecular de varianza (AMOVA a partir de los 12 STR’s, mostró que la estructuración genética no es significativa (FST de 0.032; adicionalmente se evidenció alta endogamia en la muestra mestiza de Caldas (0.43 y en la muestra indígena Coyaima (0.34.Conclusiones: Con los marcadores moleculares estudiados se estableció la estructura genética de poblaciones del sur-occidente colombiano confirmándose adicionalmente el grado de miscegenación y el flujo genético ocurrido entre diferentes grupos étnicos del centro y sur-occidente colombiano.

  19. Análisis genético del virus peruano de la fiebre amarilla

    Directory of Open Access Journals (Sweden)

    Carlos Yábar V

    2002-01-01

    Full Text Available Objetivo: Determinar las variantes genéticas de aislamientos del virus peruano de la Fiebre Amarilla (FA. Materiales y métodos: la región carboxiterminal del gen de la envoltura (E de cinco aislamientos de FA obtenidas de pacientes provenientes de Ayacucho 1978 (PER1, Junín 1995 (PER2, Cerro de Pasco (PER3, Cusco (1998 y San Martín (1999 fue amplificada por PCR, secuenciada y analizada con programas software de ADN. Resultados: el índice de similaridad de la secuencia de nucleótidos entre los cinco aislamientos reveló valores oscilantes entre 94,3% y 99,3%, mientras que la secuencia de aminoácidos presentó valores entre 97,6% y 99,7% de similaridad. El análisis filogenético demostró una distancia genética entre 0,40 y 6,50 mediante la secuencia de nucleótidos y a través de la secuencia de aminoácidos se observó un rango de 0,30 y 4,29. Sin embargo, las secuencias correspondientes a los sitios de glicosilación y a los epítopes de reconocimiento humoral fueron conservadas entre los cinco aislamientos, con excepción de algunos aislamientos de referencia reportados por otros autores. Conclusiones: los virus de FA peruanos forman un grupo filogenético distinto a otros virus de FA sudamericanos, basados en el análisis genéticos del gen E.

  20. Resgate vegetativo por alporquia de genótipos adultos de urucum (Bixa orellana L.

    Directory of Open Access Journals (Sweden)

    Nilton César Mantovani

    2010-09-01

    Full Text Available Este trabalho teve por objetivos avaliar a técnica de alporquia visando ao resgate vegetativo de genótipos de urucum (Bixa orellana L. e a obtenção de plantas fornecedoras de propágulos para processos de propagação clonal. Foram utilizadas dez plantas matrizes de urucum, com 12 anos de idade, obtidas partindo do cruzamento artificial entre os genótipos "Fruto Verde Piloso" X "Fruto Vermelho Liso". Os alporques foram realizados em ramos de 1 a 2 cm de diâmetro, utilizando-se como substrato uma mistura de vermiculita e musgo. Foi avaliado o efeito (1 do tipo de anelamento da casca dos ramos (total ou parcial, com 1 cm de comprimento, (2 do AIB (ácido indol-3-butírico a 0 e 4,92 mM aplicado em papel filtro e (3 do tipo de proteção dos alporques (filmes plásticos transparente ou preto ou tecido tencel, no enraizamento de alporque dos dez genótipos. A técnica de alporquia proporcionou o enraizamento de ramos dos dez genótipos avaliados, com eficiência variável de 20 a 100%, havendo efeito do genótipo sobre a frequência de enraizamento. A sobrevivência desses alporques foi de 100% após o plantio quando estes foram produzidos com anelamento total, tratados com AIB e protegidos com plástico transparente. Em casa de vegetação os alporques desenvolveram ramos partindo da brotação de gemas caulinares constituindo estoques de explantes apropriados para serem utilizados como estacas ou como fontes de segmentos nodais para a propagação in vitro desta espécie.

  1. Gen SRY y ausencia de tejido testicular en una mujer 47XYY con disgenesia gonadal.

    Directory of Open Access Journals (Sweden)

    William Jubiz

    2009-11-01

    Full Text Available Este artículo revisa conceptos actuales sobre determinación y diferenciación sexual con base en el estudio genético de una niña de 13 años que consultó por talla baja y aumento de peso. El examen físico mostró Tanner I en mamas y en vello púbico, sin signos de androgenización. Mientras el nivel de la hormona de crecimiento (GH era normal, las hormonas folículoestimulante (FSH y luteinizante (LH estaban aumentadas. Mediante laparoscopia y posterior estudio patológico se demostró la presencia de gonadas rudimentarias con ausencia de tejido testicular. Aunque el cariotipo obtenido fue 47XYY y el análisis molecular identificó la presencia del gen SRY, su funcionalidad es incierta, lo que hace necesaria su secuenciación, con la finalidad de determinar posibles mutaciones. En respuesta a la terapia con estrógenos y progesterona se desarrollaron tanto los caracteres sexuales secundarios como una menstruación normal. Aunque es posible que en la paciente haya una doble alteración genética donde concurran la mutación de novo de un gen y una no disyunción en la meiosis paterna, el caso descrito es ilustrativo de la importancia del estudio genético en la evaluación de la disgenesia gonadal.

  2. Genética molecular de caracteres cuantitativos en cruzamientos dialélicos de tomate

    Directory of Open Access Journals (Sweden)

    Guillermo Raúl Pratta

    2011-05-01

    Full Text Available El objetivo de este trabajo fue evaluar marcadores moleculares y caracteres cuantitativos en un cruzamiento dialélico completo sin recíprocos, entre cinco líneas recombinantes de tomate y sus híbridos. Se obtuvieron perfiles de AFLP ("amplified fragment length polymorphism" y de polipéptidos del pericarpio en cuatro estados de madurez del fruto de 15 genotipos. Se evaluaron, entre otros: peso, acidez titulable, pH, vida poscosecha y firmeza. Se calculó el porcentaje de polimorfismo para los marcadores moleculares y el porcentaje de variabilidad genética para los caracteres cuantitativos en el grupo de líneas recombinantes, el de híbridos y el conjunto de genotipos. Se realizaron análisis de agrupamiento con cada nivel de variación genética. Para AFLP, el porcentaje de polimorfismo varió entre 34 y 54% y, para los perfiles polipeptídicos, entre 40 y 78%. Mayor polimorfismo fue observado en el grupo de híbridos. La variabilidad genética fue de 100% para acidez y 34% para firmeza, con los mayores valores en los parentales. La similitud genética varió entre los genotipos según el nivel de variación genética; pero la consistencia en el agrupamiento de algunas líneas recombinantes y sus híbridos fue conservada, lo que evidenció asociaciones entre los datos moleculares y fenotípicos.

  3. Divergência genética entre clones de guaranazeiro

    Directory of Open Access Journals (Sweden)

    Nascimento Filho Firmino José do

    2001-01-01

    Full Text Available As técnicas multivariadas, para estimar a diversidade genética de um grupo de progenitores, têm sido utilizadas com freqüência pelos melhoristas de plantas. Os progenitores são utilizados em cruzamentos biparentais ou múltiplos, para formação de populações segregantes que tenham maior probabilidade de recuperação de genótipos superiores. Este trabalho foi realizado com o objetivo de identificar clones de guaranazeiro produtivos e divergentes que possam ser utilizados em um programa de cruzamentos para obter híbridos com alto valor heterótico e materiais para propagação vegetativa. Foram avaliados 148 clones de guaranazeiro atualmente em uso no programa de melhoramento genético da Embrapa-Centro de Pesquisa Agroflorestal da Amazônia Ocidental. Utilizou-se, para estimativa da divergência genética, a análise de agrupamento, em que a medida de dissimilaridade utilizada foi a distância euclidiana média padronizada e os métodos de agrupamento de otimização de Tocher e do vizinho mais próximo para construção do dendrograma entre grupos de clones. Houve a formação de sete grupos divergentes de clones. Concluiu-se que a divergência genética entre os clones não é grande, pois dois grupos foram formados com dois clones e três grupos foram formados somente com um único clone. Os clones CMU384 e CMU801 foram os mais próximos geneticamente e podem ser utilizados na formação de uma população com desenvolvimento vegetativo uniforme para uso em plantios comerciais.

  4. Transformação genética em espécies florestais.

    Directory of Open Access Journals (Sweden)

    Claudia Studart-Guimarães

    2010-08-01

    Full Text Available A transformação genética, que compreende a introdução de genes exógenos de forma controlada no genoma de uma célula vegetal e posterior regeneração da planta transgênica, tem contribuído com os programas de melhoramento genético de plantas pela obtenção de genótipos com novas características de interesse. O melhoramento de espécies florestais é limitado por características intrínsecas a tais espécies, como a altura dos indivíduos e o ciclo longo de vida. A transformação genética constitui, portanto, uma alternativa para a obtenção de espécies florestais com características desejáveis em um menor espaço de tempo. Plantas transgênicas com resistência a determinadas pragas, com melhor qualidade de madeira, maior produção de biomassa, tolerância a herbicidas, entre outras características de interesse, já foram obtidas para diferentes espécies florestais de importância econômica como álamo, eucalipto e pinheiros em geral. Este trabalho mostra a importância da transformação genética, associada a outras técnicas biotecnológicas no melhoramento de espécies florestais, as técnicas de transformação mais utilizadas e as características que já foram introduzidas nessas espécies pela transformação.

  5. EFEKTIVITAS TRANSFER DAN ANALISIS EKSPRESI GEN IMUNOGENIK TAHAN KOI HERPES VIRUS (KHV PADA IKAN MAS (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Khairul Syahputra

    2014-04-01

    Full Text Available Penelitian transfer gen imunogenik tahan KHV (krt-GP11 pada ikan mas telah dilakukan dengan metode elektroporasi sperma menggunakan konsentrasi DNA yang berbeda. Penelitian ini bertujuan untuk mengetahui konsentrasi DNA optimal yang efektif digunakan dalam transfer gen pada ikan mas. Sperma dielektroporasi menggunakan tipe kejutan square wave dengan voltase 50 V dan jumlah kejutan tiga kali. Konsentrasi DNA yang digunakan adalah 10 μg/mL, 50 μg/mL, dan 100 μg/mL. Deteksi transgen pada sperma, embrio, dan larva dilakukan dengan metode PCR menggunakan primer spesifik untuk gen krt-GP11. Ekspresi transgen pada embrio dan larva dianalisis secara semi-kuantitatif dengan metode reverse transcriptase PCR (RT-PCR. Hasil penelitian menunjukkan bahwa gen krt-GP11 terdeteksi pada sperma, embrio, dan larva. Pemberian konsentrasi DNA 10 μg/mL lebih efektif digunakan dalam transfer gen krt-GP11 pada ikan mas, sedangkan peningkatan konsentrasi DNA yang digunakan tidak memberikan hasil yang berbeda terhadap keberhasilan transfer gen pada ikan mas. Ekspresi gen krt-GP11 yang berhasil diintroduksikan pada ikan mas baru dapat teramati dengan baik pada fase embrio.

  6. GenGIS 2: Geospatial Analysis of Traditional and Genetic Biodiversity, with New Gradient Algorithms and an Extensible Plugin Framework

    Science.gov (United States)

    Parks, Donovan H.; Mankowski, Timothy; Zangooei, Somayyeh; Porter, Michael S.; Armanini, David G.; Baird, Donald J.; Langille, Morgan G. I.; Beiko, Robert G.

    2013-01-01

    GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index) for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA), which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis. PMID:23922841

  7. GenGIS 2: geospatial analysis of traditional and genetic biodiversity, with new gradient algorithms and an extensible plugin framework.

    Directory of Open Access Journals (Sweden)

    Donovan H Parks

    Full Text Available GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA, which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis.

  8. Genética Molecular das Epidermólises Bolhosas Molecular Genetics of Epidermolysis Bullosa

    Directory of Open Access Journals (Sweden)

    Hiram Larangeira de Almeida Jr

    2002-10-01

    Full Text Available O estudo das alterações moleculares das epidermólises bolhosas tem contribuído para que se compreenda melhor essas enfermidades. Na epidermólise bolhosa simples a maioria dos casos está associada com alteração nas citoqueratinas basais 5 (gen KRT5 e 14 (gen KRT14, o que modifica o citoesqueleto na camada basal da epiderme, levando à degeneração dessa camada, formando bolha intra-epidérmica. Mutações na plectina (gen PLEC1, componente da placa interna do hemidesmossoma, levam também à clivagem intra-epidérmica. Na epidermólise bolhosa juncional vários gens estão envolvidos, em decorrência da complexidade da zona da membrana basal, todos levando ao descolamento dos queratinócitos basais na lâmina lúcida, pela disfunção da aderência entre esses e a lâmina densa. Alterações na laminina 5 (gens LAMA3, LAMB3 e LAMC2, integrina alfa6beta4 (gens ITGA6 e ITGB4 e colágeno XVII (gen COL17A1 foram descritas. Por fim, na epidermólise bolhosa distrófica apenas um gen está mutado, alterando o colágeno VII (gen COL7A1, principal componente das fibrilas ancorantes, produzindo clivagem abaixo da lâmina densa, variando fenotipicamente de acordo com a conseqüência da mutação. Outra aplicação importante dessas informações refere-se ao diagnóstico pré-natal, com a perspectiva no futuro da terapia gênica.New data regarding the molecular aspects of the heterogeneous group of epidermolysis bullosa has brought some important information about its pathogenesis. In epidermolysis bullosa simplex the majority of mutations are localized in the genes of the basal cytokeratin 5 (gene KRT5 and 14 (gene KRT14, cytolysis at this layer with intraepidermal blister is seen under light microscopy. Mutations of plectin (gene PLEC1, a protein found in the inner hemidesmosomal plaque, leads also to intraepidermal blisters. In junctional epidermolysis bullosa many proteins from the basal membrane zone are involved, such as laminin 5 (genes

  9. El uso de los tests genéticos por parte de las compañías de seguros

    OpenAIRE

    César Rodríguez Orgaz

    2011-01-01

    Se aborda la discusión en torno a la pertinencia del uso de tests genéticos por parte de las compañías de seguros. La idea que se defiende es la siguiente: si decidimos regular el recurso a los tests genéticos fuera del ámbito sanitario, que sea para hacer justicia a los derechos de terceras personas y en ningún caso por miedo a posibles discriminaciones. Se trata de llamar la atención sobre los riesgos aparejados al uso irresponsable de los datos genéticos por parte de las compañías de segur...

  10. Diversidad genética en Salvia elegans Vahl. (Lamiaceae), poblaciones con potencial para restauración

    OpenAIRE

    Bedolla García, Brenda Yudith

    2012-01-01

    El conocimiento de la estructura genética poblacional y diversidad genética de las especies es necesario para un buen manejo en conservación y/o restauración. Salvia elegans es una hierba leñosa que esta presente en bosques de coníferas distribuida a lo largo del Eje Neovolcánico. Se determinó la estructura genética de 8 poblaciones en S. elegans mediante la técnica de marcadores moleculares RAPD con 10 oligonucleótidos, que generaron 158 bandas, de las cuales el 95.5% fueron p...

  11. Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae, as Phaseolibacter flectens gen. nov., comb. nov.

    Science.gov (United States)

    Halpern, Malka; Fridman, Svetlana; Aizenberg-Gershtein, Yana; Izhaki, Ido

    2013-01-01

    Pseudomonas flectens Johnson 1956, a plant-pathogenic bacterium on the pods of the French bean, is no longer considered to be a member of the genus Pseudomonas sensu stricto. A polyphasic approach that included examination of phenotypic properties and phylogenetic analyses based on 16S rRNA, rpoB and atpD gene sequences supported the transfer of Pseudomonas flectens Johnson 1956 to a new genus in the family Enterobacteriaceae as Phaseolibacter flectens gen. nov., comb. nov. Two strains of Phaseolibacter flectens were studied (ATCC 12775(T) and LMG 2186); the strains shared 99.8 % sequence similarity in their 16S rRNA genes and the housekeeping gene sequences were identical. Strains of Phaseolibacter flectens shared 96.6 % or less 16S rRNA gene sequence similarity with members of different genera in the family Enterobacteriaceae and only 84.7 % sequence similarity with Pseudomonas aeruginosa LMG 1242(T), demonstrating that they are not related to the genus Pseudomonas. As Phaseolibacter flectens formed an independent phyletic lineage in all of the phylogenetic analyses, it could not be affiliated to any of the recognized genera within the family Enterobacteriaceae and therefore was assigned to a new genus. Cells were Gram-negative, straight rods, motile by means of one or two polar flagella, fermentative, facultative anaerobes, oxidase-negative and catalase-positive. Growth occurred in the presence of 0-60 % sucrose. The DNA G+C content of the type strain was 44.3 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, Pseudomonas flectens Johnson 1956 is transferred to the novel genus Phaseolibacter gen. nov. as Phaseolibacter flectens gen. nov., comb. nov. The type strain of Phaseolibacter flectens is ATCC 12775(T) = CFBP 3281(T) = ICMP 745(T) = LMG 2187(T) = NCPPB 539(T).

  12. Divergência genética entre cinco genótipos de melão rendilhado Genetic divergence among five muskmelon cultivars

    Directory of Open Access Journals (Sweden)

    Adriana Antonieta do Nascimento Rizzo

    2002-06-01

    Full Text Available Estimou-se a divergência genética entre cinco genótipos de melão rendilhado (Cucumis melo var. reticulatus Naud. (JAB-20, JAB-21, JAB-22, JAB-23 e 'Bônus nº 2' e determinou-se qual a contribuição relativa das 16 características avaliadas [nº médio de flores masculinas, hermafroditas/planta; produção total de frutos/m², peso médio dos frutos comerciáveis; diâmetro médio transversal e longitudinal do fruto (DMTF e DMLF; diâmetro médio transversal da inserção do pedúculo (DMTP; espessura média do mesocarpo e epicarpo (EMM e EME; diâmetro médio longitudinal e transversal do lóculo (DMTL e DMLL; proporção da cavidade (PC; desprendimento de sementes (DS; teor de sólidos solúveis totais (SST, pH e acidez titulável (AT] na divergência gen��tica. Obtiveram-se dois grupos de similaridade: I- JAB-20, JAB-21 e 'Bônus nº2' e II- JAB-22 e JAB-23. As características DMLF, DMTP, DMLL, DS e SST foram as que mais contribuíram para a divergência genética entre os genótipos.The genetic divergence of five cultivars of muskmelon was estimated (Cucumis melo var. reticulatus Naud (JAB-20, JAB-21, JAB-22, JAB-23 and 'Bônus nº2' and the relative contribution of each 16 characteristics were determined (number of male flowers per plant; total production of fruit, weight of fruits; longitudinal and transversal diameters of fruits; thickness and color of flesh and skin; longitudinal and transversal loculos diameter of fruits; seed loosing; netting thickness; and % total solvers solids, pH and total acidity in genetic divergence. Two groups of similarity were formed between the genitors by the values of D², one of then was constituted of the JAB-20 and JAB-21 and 'Bônus nº 2' genotypes, and another of the JAB-22 and JAB-23. The characteristics of longitudinal loculos diameters, longitudinal diameter of fruits, transversal diameter of peduncle insertion, % total solvers solids and seed loosing contributed to for genetic

  13. Genética y genómica de la hipertensión arterial: una actualización

    OpenAIRE

    Ramírez-Bello, Julián; Pérez-Méndez,Oscar; Ramírez-Fuentes,Silvestre; Carrillo-Sánchez,Silvia; Vargas-Alarcón, Gilberto; Fragoso, José Manuel

    2011-01-01

    La hipertensión arterial es considerada la principal causa de riesgo para el desarrollo de infarto agudo al miocardio, falla cardiaca, arritmia ventricular, nefropatía, ceguera, entre otras. La hipertensión arterial es una enfermedad multifactorial en la que participan factores ambientales, genéticos e intrínsecos como raza y género. La Organización Mundial de la Salud ha estimado que la prevalencia de la hipertensión se incrementará drásticamente, de modo que en la próxima década será la pri...

  14. Variabilidad genética de la respuesta inflamatoria. I. Polimorfismo -511 C/T en el gen IL1β en diferentes subpoblaciones peruanas

    OpenAIRE

    Óscar Acosta; Luis Solano; Doris Huerta; Daniel Oré; José Sandoval; José Figueroa; Ricardo Fujita

    2012-01-01

    Anales de la Facultad de Medicina es una publicación de la Universidad Nacional Mayor de San Marcos. El polimorfismo -511 citosina/timina (-511 C/T) en la región promotora del gen interleuquina 1 beta (IL1β) está implicado en la producción diferencial de la citoquina y por tanto puede estar asociado a la respuesta inmuno-inflamatoria en obesidad, dislipidemias, cardiopatías, cáncer, infecciones, y el tratamiento con nutrientes y fármacos. Objetivos: Establecer la distribución de frec...

  15. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  16. Polimorfismos del gen BoLA-DRB3.2* en ganado criollo colombiano

    Directory of Open Access Journals (Sweden)

    Darwin Hernández H.

    2013-10-01

    Full Text Available Objetivo. Caracterizar el polimorfismo del gen BoLA-DRB3.2* en las razas bovinas criollas y colombianas. Materiales y métodos. En 360 muestras de ADN de ocho razas bovinas criollas (Blanco Orejinegro, Casanareño, Costeño con Cuernos, Chino Santandereano, Caqueteño, Hartón del Valle, Romosinuano y San Martinero, dos razas sintéticas Colombianas (Lucerna y Velásquez y dos razas foráneas (Brahman y Holstein se evaluó el polimorfismo del gen BoLA-DRB3.2 mediante técnicas moleculares (PCR-RFLP; se calculó el número promedio de alelos (NPA, las frecuencias, la heterocigocidad esperada (He y observada (Ho, el equilibrio de Hardy-Weinberg, la estructura genética y los valores de FST y FIS. Resultados. El NPA fue 14.6 ± 3.8 siendo Caqueteño la raza con mayor NPA (25 y el menor el Chino Santandereano (10. Se encontraron 41 alelos BoLA-DRB3.2* los más frecuentes fueron *28, *37, *24, *23, *20, *27, *8, *16, *39 (0.17, 0.11, 0.10, 0.09, 0.09, 0.07, 0.07 y 0.06 respectivamente. Se encontró alta diversidad genética (He = 0.878 con mayor valor en Caqueteño (0.96 y menor en San Martinero (0.81. Todas las razas se encontraron en equilibrio de Hardy-Weinberg, se encontraron valores altamente significativos de diferenciación genética (FST= 0.044 y de coeficiente de endogamia (FIS = 0.249. Conclusiones. El ganado criollo colombiano posee alto polimorfismo del gen BoLA-DRB3.2* representado en los altos valores de NPA y diversidad génetica.

  17. Cordiamyia globosa gen.n. e sp.n. (Diptera, Cecidomyiidae, Cecidomyiidi associado com Cordia Verbenacea DC. (Boraginaceae no Brasil Cordiamyia globosa gen.n. and sp.n. (Diptera, Cecidomyiidae associated with Cordia verbekacea DC. (Boraginaceae in Brazil

    Directory of Open Access Journals (Sweden)

    Valéria Cid Maia

    1996-01-01

    Full Text Available Cordiamyia globosa gen.n., sp.n. (Diptera, Cecidomyiidae, Cecidomyiidi associated with Cordia verbenacea (Boraginaceae, in Brazil, is described and illustrated (larva, pupa, male, female and gall.

  18. Genética comunitária : a inserção da genética médica na atenção primária à saúde em Porto Alegre

    OpenAIRE

    Taiane Alves Vieira

    2012-01-01

    Introdução: Com o melhor controle das causas ambientais, as doenças genéticas e as malformações congênitas cada vez mais ganham destaque como fatores morbidade e mortalidade, assim como cada vez mais se reconhece a importância da base genética para as doenças comuns. Desta forma, é necessário desenvolver estratégias de prevenção e controle destas condições, assim como acesso aos cuidados de saúde para pessoas com ou em risco de desenvolver uma doença genética. A integração da genética na Aten...

  19. GenBank数据库和PubMed数据库中序列数据信息检索比较%Comparison of sequential data retrieval results from GenBank and PubMed

    Institute of Scientific and Technical Information of China (English)

    李轶

    2009-01-01

    通过对GenBank数据库和PubMed数据库的数据来源、检索界面和检索结果等的对比分析,发现2个检索库检索的序列数据信息存在差别,GenBank数据库检索结果和检准率均高于PubMed数据库.

  20. Nitridogermanate nitrides Sr7[GeN4]N2 and Ca7[GeN4]N2: synthesis employing sodium melts, crystal structure, and density-functional theory calculations.

    Science.gov (United States)

    Junggeburth, Sebastian C; Oeckler, Oliver; Johrendt, Dirk; Schnick, Wolfgang

    2008-12-15

    The alkaline earth nitridogermanate nitrides AE(7)[GeN(4)]N(2) (AE = Ca, Sr) have been synthesized using a Na flux technique in sealed Ta tubes. According to single-crystal X-ray diffraction the isotypic compounds crystallize in space group Pbcn (No. 60) with Z = 4, (Sr(7)[GeN(4)]N(2): a = 1152.6(2), b = 658.66(13), c = 1383.6(3) pm, V = 1050.5(4) x 10(6) pm(3), R1 = 0.049; Ca(7)[GeN(4)]N(2): a = 1082.6(2), b = 619.40(12), c = 1312.1(3) pm, V = 879.8(3) x 10(6) pm(3), R1 = 0.016). Owing to the high N/Ge ratio, the compounds contain discrete N(3-) ions coordinated by six AE(2+) besides discrete [GeN(4)](8-) tetrahedrons. One of the AE(2+) ion is coordinated by only four N(3-) ions, which is rather an unusual low coordination number for Sr(2+). Together with the isolated [GeN(4)](8-) tetrahedrons, these Sr(2+) ions form chains of alternating cation centered edge sharing tetrahedrons. The electronic structure and chemical bonding in Sr(7)[GeN(4)]N(2) has been analyzed employing linear muffin-tin orbital (LMTO) band structure calculations.

  1. TopoGen: A Network Topology Generation Architecture with application to automating simulations of Software Defined Networks

    CERN Document Server

    Laurito, Andres; The ATLAS collaboration

    2017-01-01

    Simulation is an important tool to validate the performance impact of control decisions in Software Defined Networks (SDN). Yet, the manual modeling of complex topologies that may change often during a design process can be a tedious error-prone task. We present TopoGen, a general purpose architecture and tool for systematic translation and generation of network topologies. TopoGen can be used to generate network simulation models automatically by querying information available at diverse sources, notably SDN controllers. The DEVS modeling and simulation framework facilitates a systematic translation of structured knowledge about a network topology into a formal modular and hierarchical coupling of preexisting or new models of network entities (physical or logical). TopoGen can be flexibly extended with new parsers and generators to grow its scope of applicability. This permits to design arbitrary workflows of topology transformations. We tested TopoGen in a network engineering project for the ATLAS detector ...

  2. No quenching of magnetic moment for the GenCo (n=1-13) clusters: First-principles calculations

    Science.gov (United States)

    Jing, Qun; Tian, Fu-yang; Wang, Yuan-xu

    2008-03-01

    The authors predict that for the GenCo (n=1-13) clusters the magnetic moment does not quench, which is dark contrast to the previous results with transition-metal-doped Sin clusters. It may be due to the unpaired electrons of the Co atom in the clusters. For the ground state structures of the GenCo (n⩾9) clusters, the Co atom completely falls into the center of the Ge outer frame, forming metal-encapsulated Gen cages. The doping of the Co atom enhances the stability of the host Gen clusters. The Ge10Co cluster with the bicapped tetragonal antiprism structure is more stable than others, which agrees very well with the results of the experiment of the Co /Ge binary clusters by the laser vaporization.

  3. El uso de los tests genéticos por parte de las compañías de seguros

    Directory of Open Access Journals (Sweden)

    César Rodríguez Orgaz

    2011-01-01

    Full Text Available Se aborda la discusión en torno a la pertinencia del uso de tests genéticos por parte de las compañías de seguros. La idea que se defiende es la siguiente: si decidimos regular el recurso a los tests genéticos fuera del ámbito sanitario, que sea para hacer justicia a los derechos de terceras personas y en ningún caso por miedo a posibles discriminaciones. Se trata de llamar la atención sobre los riesgos aparejados al uso irresponsable de los datos genéticos por parte de las compañías de seguros, y se llega a la conclusión de que un uso tal compromete los derechos de terceras personas implicadas. Por último, plantearemos posibles escenarios para el futuro sobre el procesamiento de la información genética

  4. Squamasnema amazonica n. gen. n. sp. (Heligmonellinae): A new parasite of Proechimys roberti (Rodentia: Echimyidae) in the Brazilian Amazon.

    Science.gov (United States)

    Cordeiro, Helrik da Costa; Melo, Francisco Tiago de Vasconcelos; Furtado, Adriano Penha; Giese, Elane Guerreiro; Maldonado, Arnaldo; dos Santos, Jeannie Nascimento

    2015-08-01

    A new species of nematode, Squamasnema amazonica n. gen. n. sp., is described based on specimens found parasitizing the small intestine of Proechimys roberti (Rodentia: Echimyidae) collected during a survey of the fauna of Tapirapé-Aquirí National Forest (Brazil, Eastern Brazilian Amazon). The nematodes were fixed and processed for light microscopy and scanning electron microscopy (SEM). These nematodes were classified under the family Heligmonellidae and the subfamily Heligmonellinae. Although several species in the family Heligmonellidae exhibit discontinuous ridges, Squamasnema n. gen. and Trichotravassosia are the only genera with columns of scales along their entire body, as an apomorphy of the synlophe. Squamasnema n. gen. has columns of cuticular cells along its body, except for on the left flank, and exhibits a synlophe with no size gradient or inclination and does not present chitinized structures supporting the synlophe. Therefore, due to these morphological differences of Squamasnema n. gen., the creation of a new genus was necessary.

  5. Diversidad genética de Plasmodium falciparum y sus implicaciones en la epidemiología de la malaria

    National Research Council Canada - National Science Library

    Judy Natalia Jiménez; Carlos Enrique Muskus; Iván Darío Vélez

    2005-01-01

    La diversidad genética le confiere a Plasmodium falciparum la capacidad de evadir la respuesta inmune del hospedero y producir variantes resistentes a medicamentos y a vacunas, aspectos que juegan un papel importante...

  6. Desafios para a universalização da genética clínica: o caso brasileiro

    OpenAIRE

    2011-01-01

    Acesso restrito: Texto completo. p. 61-68 Este artigo aborda as dificuldades de inserir a genética médica como parte do Sistema Único de Saúde (SUS) no Brasil. Em 2009, foi instituída no Brasil a Política Nacional de Atenção Integral em Genética Médica, cujo pilar central seria o aconselhamento genético. Porém, são problemas estratégicos para a implementação dessa política a falta de programas de formação em aconselhamento genético, o desconhecimento acerca de quantos profissionais existem...

  7. Estudi comparatiu de l'estructura del gen "Adh" a vàries espècies de "Drosophila"

    OpenAIRE

    Marfany i Nadal, Gemma

    1991-01-01

    S'ha caracterizat l'estructura de la regió genòmica del gen "Adh" a quatre espècies del gènere "Drosophila", pertanyents al subgrup "obscura": "D. ambigua", "D. subobscura", "D. madeirensis" i "D. guanche" amb els objectius d'analitzar l'organització i l'evolució d'aquesta regió genòmica dins d'aquest gènere i clarificar les relacions filogenètiques d'aquestes espècies. Per assolir aquestes fites, es van construir llibreries genòmiques de cada espècie que es van crivellar amb una sonda que co...

  8. Simulation of an extended surface detector IceVeto for IceCube-Gen2

    Energy Technology Data Exchange (ETDEWEB)

    Hansmann, Tim; Auffenberg, Jan; Haack, Christian; Hansmann, Bengt; Kemp, Julian; Konietz, Richard; Leuner, Jakob; Raedel, Leif; Stahlberg, Martin; Schoenen, Sebastian; Wiebusch, Christopher [III. Physikalisches Institut B, RWTH Aachen University (Germany); Collaboration: IceCube-Collaboration

    2016-07-01

    IceCube is a neutrino observatory located at the geographic South Pole. The main backgrounds for IceCube's primary goal, the measurement of astrophysical neutrinos, are muons and neutrinos from cosmic-ray air showers in the Earth's atmosphere. Strong supression of these backgrounds from the Southern hemisphere has been demonstrated by coincident detection of these air showers with the IceTop surface detector. For an extended instrument, IceCube-Gen2, it is considered to build an enlarged surface array, IceVeto, that will improve the detection capabilities of coincident air showers. We will present simulation studies to estimate the IceVeto capabilities to optimize the IceCube-Gen2 design.

  9. Simulating GenCo bidding strategies in electricity markets with an agent-based model

    Energy Technology Data Exchange (ETDEWEB)

    Botterud, Audun; Thimmapuram, Prakash R.; Yamakado, Malo

    2005-12-15

    In this paper we use an agent-based simulation model, EMCAS, to analyze market power in electricity markets. We focus on the effect of congestion management on the ability of generating companies (GenCos) to raise prices beyond competitive levels. An 11-node test power system is used to compare a market design based on locational marginal pricing with a market design that uses system marginal pricing and congestion management by counter trading. Bidding strategies based on both physical and economic withholding are compared to a base case with production cost bidding. The results show that unilateral market power is exercised under both pricing mechanisms. However, the largest changes in consumer costs and GenCo profits due to strategic bidding occur under the locational marginal pricing scheme. The analysis also illustrates that agent-based modeling can contribute important insights into the complex interactions between the participants in transmission-constrained electricity markets. (Author)

  10. EL GEN PIG-A COMO INDICADOR DE MUTACIONES SOMÁTICAS In vivo

    OpenAIRE

    Mercedes Monserrat PACHECO MARTÍNEZ; Elsa CERVANTES RÍOS; María Del Carmen GARCÍA RODRÍGUEZ; Alda Rocío ORTIZ MUÑIZ

    2014-01-01

    La medición cuantitativa de las mutaciones es fundamental en el campo de la mutagé - nesis ambiental. Recientemente se han descrito nuevos métodos para su valoración y para la evaluación de mutaciones inducidas por agentes químicos in vivo . Uno de ellos está basado en el análisis del fenotipo relacionado con la expresión del gen fosfatidi - linositol glicano de clase A ( PIG-A en humanos y Pig-a en roedores). Este gen está ligado al cromosoma X y es esencial para la síntesis del ancla glicos...

  11. EFEKTIVITAS METODE TRANSFEKSI DALAM TRANSFER GEN PADA ZIGOT IKAN CUPANG ALAM (WILD BETTA, Betta imbellis

    Directory of Open Access Journals (Sweden)

    Anjang Bangun Prasetio

    2013-08-01

    dan 3:1 dengan jumlah ulangan masing-masing sebanyak enam kali. Sebagai kontrol, ditambahkan juga perlakuan non transfeksi (non transgenik yaitu tanpa penyisipan gen GFP maupun RFP. Pengamatan dilakukan sejak perkembangan zigot mulai dari penghitungan derajat penetasan (HR dan sintasan larva (SR. Hasil penelitian menunjukkan bahwa setelah dilakukan transfeksi tidak memperlihatkan pola yang jelas dari setiap perlakuan, namun secara umum tidak berbeda signifikan dengan kontrol non transgenik. PCR pada embrio dan larva menunjukkan hasil positif di mana DNA teramplifikasi pada ukuran sekitar 0,6 kb untuk beberapa ulangan. Dari hasil yang diperoleh ini dapat ditarik kesimpulan bahwa metode transfeksi efektif digunakan untuk transfer gen ikan cupang alam, wild betta (Betta imbellis.

  12. Gen 2.0 Mixer/Ejector Nozzle Test at LSAF June 1995 to July 1996

    Science.gov (United States)

    Arney, L. D.; Sandquist, D. L.; Forsyth, D. W.; Lidstone, G. L.; Long-Davis, Mary Jo (Technical Monitor)

    2005-01-01

    Testing of the HSCT Generation 2.0 nozzle model hardware was conducted at the Boeing Low Speed Aeroacoustic Facility, LSAF. Concurrent measurements of noise and thrust were made at critical takeoff design conditions for a variety of mixer/ejector model hardware. Design variables such as suppressor area ratio, mixer area ratio, liner type and thickness, ejector length, lobe penetration, and mixer chute shape were tested. Parallel testing was conducted at G.E.'s Cell 41 acoustic free jet facility to augment the LSAF test. The results from the Gen 2.0 testing are being used to help shape the current nozzle baseline configuration and guide the efforts in the upcoming Generation 2.5 and 3.0 nozzle tests. The Gen 2.0 results have been included in the total airplane system studies conducted at MDC and Boeing to provide updated noise and thrust performance estimates.

  13. INTRODUKSI GEN Sitrat Sintase KE DALAM RUMPUT LAUT Kappaphycus alvarezii MENGGUNAKAN Agrobacterium tumefaciens

    Directory of Open Access Journals (Sweden)

    Ristanti Frinra Daud

    2013-08-01

    ekonomis penting. Ice-ice merupakan penyakit yang paling umum menyerang rumput laut dan menyebabkan menurunnya produksi rumput laut. Penyakit ini disebabkan oleh perubahan salinitas, suhu, dan pencemaran logam berat. Asam sitrat digunakan sebagai pengkelat logam berat. Introduksi gen sitrat sintase ke dalam genom tanaman diketahui dapat mengurangi cekaman oksidatif. Penelitian ini bertujuan untuk mengintroduksi gen sitrat sintase ke dalam genom K. alvarezii menggunakan perantara Agrobacterium tumefaciens. Berdasarkan eksplan yang tahan pada media seleksi higromisin, efisiensi transformasi pada K. alvarezii sebesar 7,5%. Efisiensi regenerasi tunas transgenik putatif sebesar 100%, efisiensi tunas non transgenik sebesar 100%. Analisis molekular menggunakan teknik PCR, satu dari lima K. alvarezii transgenik putatif mengandung transgen PaCS di bawah kendali promoter 35S CaMV.

  14. Els gens zombis continuen la vida més enllà de la mort

    OpenAIRE

    Bueno i Torrens, David, 1965-

    2016-01-01

    Periòdicament, algunes sèries de televisió, el cinema o la literatura juvenil han posat de moda els zombis. Que ningú es confongui, els zombis són pura ficció i no tenen cap validesa científica. Però un estudi recent indica que, després de morir, a les cèl·lules del difunt s'activen alguns programes genètics que es mantenen en funcionament diversos dies després del decés. En aquest procés hi hauria més de mil gens implicats, segons els autors de l'estudi, un equip format per investigadors del...

  15. Internal combustion engine report: Spark ignited ICE GenSet optimization and novel concept development

    Energy Technology Data Exchange (ETDEWEB)

    Keller, J.; Blarigan, P. Van [Sandia National Labs., Livermore, CA (United States)

    1998-08-01

    In this manuscript the authors report on two projects each of which the goal is to produce cost effective hydrogen utilization technologies. These projects are: (1) the development of an electrical generation system using a conventional four-stroke spark-ignited internal combustion engine generator combination (SI-GenSet) optimized for maximum efficiency and minimum emissions, and (2) the development of a novel internal combustion engine concept. The SI-GenSet will be optimized to run on either hydrogen or hydrogen-blends. The novel concept seeks to develop an engine that optimizes the Otto cycle in a free piston configuration while minimizing all emissions. To this end the authors are developing a rapid combustion homogeneous charge compression ignition (HCCI) engine using a linear alternator for both power take-off and engine control. Targeted applications include stationary electrical power generation, stationary shaft power generation, hybrid vehicles, and nearly any other application now being accomplished with internal combustion engines.

  16. Transhumanistas y Bioconservadores en torno al dopaje genético

    Directory of Open Access Journals (Sweden)

    Raúl Francisco Sebastián Solanes

    2013-04-01

    Full Text Available En el presente texto proponemos una reflexión sobre uso de tecnologías genéticas que  aumentan el rendimiento deportivo como futuro campo de investigación de una Neuroética social. Este problema, que se ha dado en llamar “dopaje genético”, congrega a su alrededor un debate entre los partidarios del Bioconservadurismo y del Transhumanismo. Expondremos la concepción de dos importantes representantes del Transhumanismo (J. Savulescu y C. Tamburrini y de dos conocidos partidarios del Bioconservadurismo (M. Sandel y R. L. Simon, a fin de subrayar la importancia de este debate y las futuras implicaciones en la mejora del rendimiento físico, cognitivo y educacional a las que se deberá hacer frente desde el nivel socio-cultural de la Neuroética.

  17. Genética en humana en Estomatología

    OpenAIRE

    2014-01-01

    El creciente interés que están despertando las enfermedades genéticas dentro del contexto de la patología humana, se debe al espectacular avance que han experimentado las cada vez más complejas metodologías que con un número día a día mayor de científicos investigan los mecanismos de la herencia. Es así como novedosos estudios bioquímicos, cromosómicos y poblaciones, han permitido reconocer en muchas entidades de causalidad confusa hasta no hace mucho, una clara etiología genética. El crec...

  18. Comportamiento productivo de cerdos portadores del gen del halotano en condiciones medioambientales no controladas

    Directory of Open Access Journals (Sweden)

    Sánchez-Chiprés, David Román

    2008-05-01

    Full Text Available El síndrome de estrés porcino es una enfermedad genética que afecta acerdos de crecimiento rápido, manifestándose con muerte súbita durante las condiciones de manejo o al sacrificio, como carne pálida, suave y exudativa. Se econoce que el síndrome de estrés porcino (PSS, el síndrome de la carne pálida suave y exudativa (PSE así como la hipertermia maligna (MH, comparten una mutación en el gen receptor de la rianodina (RYR1. Un método de diagnóstico basado en DNA y el uso de la técnica de reacción en cadena de la polimerasa, permite hacer una distinción de los genotipos para el locus halotano.

  19. Frecuencia de algunas enfermedades genéticas en Neuropediatría

    OpenAIRE

    Tatiana Zaldívar Vaillant; Nicolás Garófalo Gómez; José Vargas Díaz; Edelsia Rojas Massipe; Lucía Margarita Novoa López; Vivian Bermúdez Linares; Ivonne Martín Hernández; Carlos Ignacio Viñas Portilla

    2012-01-01

    Introducción: las enfermedades neurológicas en Pediatría son diversas y obedecen a un gran número de causas: infecciosas, genéticas, metabólicas y degenerativas, entre otras. El diagnóstico genético, dentro del método clínico en Neurología, está relacionado con el diagnóstico etiológico. Existen muy pocas publicaciones que reflejen la frecuencia de las enfermedades neurogenéticas como grupo etiológico. Objetivo: describir la frecuencia de algunas enfermedades neuropediátricas en la Consulta d...

  20. Mucopolisacaridosis II: nueva mutación patogénica en gen IDS

    Directory of Open Access Journals (Sweden)

    Eugenia Pérez-Elizondo

    2014-12-01

    Full Text Available La mucopolisacaridosis tipo II es una enfermedad lisosomal producida por la deficiencia de la enzima iduronato 2 sulfatasa. Es una condición infrecuente de herencia recesiva ligada al X, que puede producir importante discapacidad progresiva. El análisis molecular es una técnica útil en la confirmación diagnóstica, que además permite detección de portadores asintomáticos, brindando la oportunidad de asesoría genética. Se presenta el caso de un paciente con mucopolisacaridosis tipo II, en quien se documentó una nueva mutación patogénica en el Gen IDS.

  1. Thermal stability study for candidate stainless steels of GEN IV reactors

    Energy Technology Data Exchange (ETDEWEB)

    Simeg Veternikova, J., E-mail: jana.veternikova@stuba.sk [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Degmova, J. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Pekarcikova, M. [Institute of Materials Science, Faculty of Materials Science and Technology, Slovak University of Technology, Paulinska 16, 917 24 Trnava (Slovakia); Simko, F. [Department of Molten Salts, Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 36 Bratislava (Slovakia); Petriska, M. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Skarba, M. [Slovak University of Technology, Vazovova 5, 812 43 Bratislava (Slovakia); Mikula, P. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Pupala, M. [Department of Molten Salts, Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 36 Bratislava (Slovakia)

    2016-11-30

    Highlights: • Thermal resistance of advanced stainless steels were observed at 1000 °C. • GEN IV candidate steels were confronted to classic AISI steels. • ODS AISI 316 has weaker thermal resistance than classic AISI steel. • Ferritic ODS steels and NF 709 has better thermal resistance than AISI steels. - Abstract: Candidate stainless steels for GEN IV reactors were investigated in term of thermal and corrosion stability at high temperatures. New austenitic steel (NF 709), austenitic ODS steel (ODS 316) and two ferritic ODS steels (MA 956 and MA 957) were exposed to around 1000 °C in inert argon atmosphere at pressure of ∼8 MPa. The steels were further studied in a light of vacancy defects presence by positron annihilation spectroscopy and their thermal resistance was confronted to classic AISI steels. The thermal strain supported a creation of oxide layers observed by scanning electron microscopy (SEM).

  2. La política internacional de acceso a los recursos genéticos

    Directory of Open Access Journals (Sweden)

    Martha Isabel Gómez Lee

    2007-11-01

    Full Text Available La biodiversidad es un asunto político. ¿Por qué es importante el acceso e intercambio de los recursos genéticos, y cómo se relaciona con las controversias sobre propiedad intelectual y con las negociaciones ambientales y comerciales en el ámbito internacional? La respuesta a estos interrogantes pone en evidencia un conjunto de reglas, interrelaciones internacionales y nuevos actores que de- finen la política internacional de acceso a los recursos genéticos. El Convenio sobre Diversidad Biológica establece que la diversidad biológica está sometida a la soberanía nacional. ¿El acceso estará determinado por un sistema multilateral o por acuerdos bilaterales?

  3. Recent approaches of fish breeding /Abordagens recentes do melhoramento genético de peixes

    Directory of Open Access Journals (Sweden)

    João Costa Filho

    2012-04-01

    Full Text Available ResumoThis review aims at addressing the main issues related to genetic improvement applied to fish species with zootechnical potential, such as selection, genetic parameters and use of biotechnology as well as address the importance of this area of study for the expansion of aquaculture and its development trend. Fish breeding is one of the most important areas of study for fish production development. Important techniques are applied in order to advance genetic progress in fish breeding (e.g., individual selection, genetic parameters estimation and the use of biotechnology. Traditional breeding in fish farming has been conducted mainly through biometric analysis, which helps in choosing selection criteria and in the assessment of genetic parameters such as heritability and correlations between interesting traits. The use of biotechnology in fish breeding has been successful; it is an important strategy that allows breeding results to be achieved faster, although at a greater cost than traditional breeding. Fish breeding in Brazil is currently in its initial stages; however, it is a field with great growth expectations, and there is still significant research to be conducted in this area.AbstractEsta revisão tem como objetivo a abordagem dos principais aspectos relacionados ao melhoramento genético aplicado em espécies de peixes com potencial zootécnico, tais como seleção, parâmetros genéticos e utilização da biotecnologia, bem como abordar a importância desta área de estudos para a ampliação da aquicultura e sua tendência de desenvolvimento. O melhoramento genético é uma das áreas prioritárias de estudo para o desenvolvimento da produção de peixes. Assim, importantes técnicas são aplicadas visando à obtenção de progresso genético no melhoramento de peixes, como por exemplo, a seleção individual, a estimativa de parâmetros genéticos e o uso da biotecnologia. O melhoramento genético tradicional na piscicultura tem

  4. Medicina Genómica Aspectos éticos, legales y sociales del Genoma Humano

    OpenAIRE

    Rodolfo E. Ávila; María Elena Samar; Leticia Díaz-Beltrán; Esteban, Francisco J.

    2011-01-01

    La Medicina Genómica es el uso de la inf ormación de los genomas y sus deriv ados (ARN, proteínas y met abolitos) que permite guiar la toma de decisiones médicas, es un c omponente clave de la medicina personalizada. La Medicina Genómica permite conocer la cartografía del genoma hum ano y proporciona una valiosa información a tener en cuenta a la hora de detect ar genes implicados en ciert as enfermedades. Esto conlleva a que en la actualidad nos centremos más en la predicción de patologías q...

  5. Manipulação genética e dignidade humana

    OpenAIRE

    Oliveira, Simone Born de

    2001-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas Um tema como a manipulação genética e dignidade humana: da bioética ao direito, tem por fim verificar o desenvolvimento da pesquisa científica na área da genética, com suas manipulações e respectivas conseqüências sobre a dignidade humana, a bioética e o direito. Tem que ser possível definir a influência da ética e do direito no desenvolvimento das pesquisas científicas. Para definir essas possibi...

  6. Causas genéticas de deficiência de ferro

    OpenAIRE

    Saad,Sara Teresinha O.

    2010-01-01

    As causas genéticas de deficiência de ferro, real ou funcional, ocorrem por defeitos em muitas proteínas envolvidas na absorção e metabolismo de ferro. Neste capítulo descreveremos sucintamente causas genéticas de carência de ferro para a síntese de hemoglobina, que cursa então com anemia microcítica e hipocrômica. Ressalto que estas são alterações raras, com poucas descrições na literatura. Em alguns casos, o ferro funcional não está disponível para os eritroblastos sintetizarem hemoglobina,...

  7. Estado atual e perspectivas da genética e epidemiologia do alcoolismo

    Directory of Open Access Journals (Sweden)

    Claiton Henrique Dotto Bau

    Full Text Available O alcoolismo é um problema de saúde pública de escala mundial. O abuso e dependência combinados afetam aproximadamente 8% da população brasileira, gerando um grande custo social. O reconhecimento da existência de uma herdabilidade significativa contribuiu para o entendimento do problema como uma doença específica com origem biológica. Os avanços no conhecimento da neurobiologia da dependência permitiram delimitar uma série de genes candidatos para a predisposição. Atualmente, iniciam-se os estudos sobre o papel de polimorfismos genéticos na resposta ao tratamento. A integração de abordagens clínicas, epidemiológicas e de genética molecular pode identificar grupos clínicos mais responsivos a abordagens terapêuticas específicas.

  8. Pentatomiana beckerae gen. nov. and sp. nov., a new Neotropical Pentatomini (Hemiptera, Heteroptera, Pentatomidae

    Directory of Open Access Journals (Sweden)

    Jocélia Grazia

    2004-06-01

    Full Text Available A new genus, Pentatomiana, and a new species, P. beckerae, are described, based on specimens from Rio de Janeiro and Santa Catarina States, Brazil. Brachyptery is present in the males of the new species. Pentatomiana gen. nov. is compared with Lojus McDonald, 1982, which also presents brachyptery in one of the species, but in females. Illustrations of male and female external genitalia are provided.Um novo gênero, Pentatomiana, e uma nova espécie, P. beckerae, são descritos, com base em espécimes do Rio de Janeiro e de Santa Catarina, Brasil. Machos da nova espécie apresentam braquipteria. Pentatomiana gen. nov. é comparado com Lojus McDonald, 1982, o qual também apresenta braquipteria em uma das espécies, mas em fêmeas. São fornecidas ilustrações da genitália externa masculina e feminina.

  9. Dissimilaridade genética entre cultivares de mamoneira por meio de marcadores RAPD

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    Edna Lôbo Machado

    2013-03-01

    Full Text Available O objetivo deste trabalho foi identificar cultivares geneticamente divergentes de mamoneira (Ricinus communis com uso de marcadores RAPD. Um total de 58 iniciadores RAPD foi usado na genotipagem de 15 cultivares. A dissimilaridade genética entre as cultivares foi calculada a partir do índice de Jaccard, tendo-se utilizado o método da média aritmética não ponderada (UPGMA. Foram identificados 552 fragmentos, sendo 311 polimórficos (56,3%. As cultivares foram agrupadas em cinco grupos, evidência de que há divergência genética entre elas. Os marcadores moleculares do tipo RAPD são eficientes no estudo da dissimilaridade em mamoneira.

  10. MOLECULAR PHYLOGENY, ULTRASTRUCTURE, AND TAXONOMIC REVISION OF CHLOROGONIUM (CHLOROPHYTA): EMENDATION OF CHLOROGONIUM AND DESCRIPTION OF GUNGNIR GEN. NOV. AND RUSALKA GEN. NOV.(1).

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    Nakada, Takashi; Nozaki, Hisayoshi; Pröschold, Thomas

    2008-06-01

    We examined the molecular phylogeny and ultrastructure of Chlorogonium and related species to establish the natural taxonomy at the generic level. Phylogenetic analyses of 18S rRNA and RUBISCO LSU (rbcL) gene sequences revealed two separate clades of Chlorogonium from which Chlorogonium (Cg.) fusiforme Matv. was robustly separated. One clade comprised Cg. neglectum Pascher and Cg. kasakii Nozaki, whereas the other clade included the type species Cg. euchlorum (Ehrenb.) Ehrenb., Cg. elongatum (P. A. Dang.) Francé, and Cg. capillatum Nozaki, M. Watanabe et Aizawa. On the basis of unique ultrastructural characteristics, we described Gungnir Nakada gen. nov. comprising three species: G. neglectum (Pascher) Nakada comb. nov., G. mantoniae (H. Ettl) Nakada comb. nov., and G. kasakii (Nozaki) Nakada comb. nov. We also emended Chlorogonium as a monophyletic genus composed of Cg. euchlorum, Cg. elongatum, and Cg. capillatum. Because Cg. fusiforme was distinguished from the redefined Chlorogonium and Gungnir by the structure of its starch plate, which is associated with pyrenoids, we reclassified this species as Rusalka fusiformis (Matv.) Nakada gen. et comb. nov.

  11. Taxonomic revision of Chlamydomonas subg. Amphichloris (Volvocales, Chlorophyceae), with resurrection of the genus Dangeardinia and descriptions of Ixipapillifera gen. nov. and Rhysamphichloris gen. nov.

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    Nakada, Takashi; Tomita, Masaru; Wu, Jiunn-Tzong; Nozaki, Hisayoshi

    2016-04-01

    Chlamydomonas (Cd.) is one of the largest but most polyphyletic genera of freshwater unicellular green algae. It consists of 400-600 morphological species and requires taxonomic revision. Toward reclassification, each morphologically defined classical subgenus (or subgroup) should be examined using culture strains. Chlamydomonas subg. Amphichloris is characterized by a central nucleus between two axial pyrenoids, however, the phylogenetic structure of this subgenus has yet to be examined using molecular data. Here, we examined 12 strains including six newly isolated strains, morphologically identified as Chlamydomonas subg. Amphichloris, using 18S rRNA gene phylogeny, light microscopy, and mitochondria fluorescent microscopy. Molecular phylogenetic analyses revealed three independent lineages of the subgenus, separated from the type species of Chlamydomonas, Cd. reinhardtii. These three lineages were further distinguished from each other by light and fluorescent microscopy-in particular by the morphology of the papillae, chloroplast surface, stigmata, and mitochondria-and are here assigned to three genera: Dangeardinia emend., Ixipapillifera gen. nov., and Rhysamphichloris gen. nov. Based on the molecular and morphological data, two to three species were recognized in each genus, including one new species, I. pauromitos. In addition, Cd. deasonii, which was previously assigned to subgroup "Pleiochloris," was included in the genus Ixipapillifera as I. deasonii comb. nov.

  12. Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae.

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    Bondoso, Joana; Albuquerque, Luciana; Nobre, M Fernanda; Lobo-da-Cunha, Alexandre; da Costa, Milton S; Lage, Olga Maria

    2015-02-01

    Four isolates, belonging to the deep-branching phylum Planctomycetes, were recovered from the biofilm of two marine macroalgae, Ulva sp. and Laminaria sp., from the Northern coast of Portugal. These strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. They had a dimorphic cell cycle with budding reproduction. The organisms were chemoheterotrophic, strictly aerobic and mesophilic. The 16S rRNA gene sequence analysis showed that the strains belong to the family Planctomycetaceae with Rhodopirellula as the closest genus. The isolates form two separate branches (strain LF1(T) forms one branch and the strains UC8(T), UF3 and UF42 form a second branch) clearly separated from Rhodopirellula baltica with 94.2% and 93.8% 16S rRNA gene sequence similarity, respectively. Based on differential characteristics that distinguish the novel genera from R. baltica, such as cell size and shape, ultrastructure, enzymatic activities, substrate utilization pattern, fatty acid composition, phospholipid profiles and phylogeny we propose that the isolates represent two novel genera of the order Planctomycetales, Roseimaritima ulvae gen. nov., sp. nov. (type strain is UC8(T)=DSM 25454(T)=LMG 27778(T)) and Rubripirellula obstinata gen. nov., sp. nov. (type strain is LF1(T)=LMG 27779(T)=CECT 8602(T)).

  13. Two New Freshwater Woloszynskioids Asulcocephalium miricentonis gen. et sp. nov. and Leiocephalium pseudosanguineum gen. et sp. nov. (Suessiaceae, Dinophyceae) Lacking an Apical Furrow Apparatus.

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    Takahashi, Kazuya; Moestrup, Øjvind; Jordan, Richard W; Iwataki, Mitsunori

    2015-12-01

    Two new woloszynskioid dinoflagellates, Asulcocephalium miricentonis gen. et sp. nov. and Leiocephalium pseudosanguineum gen. et sp. nov., are described from Japanese freshwater ponds on the basis of bright field and fluorescence light microscopy, scanning and transmission electron microscopy, and molecular phylogeny inferred from rDNA sequences. Asulcocephalium miricentonis has a spherical anterior nucleus and chloroplast with a pyrenoid penetrated by the cytoplasm. This species has 9-12 latitudinal series of amphiesmal vesicles (AVs), including an apparently large AV on the right ventral side of the epicone. Leiocephalium pseudosanguineum has a U-shaped nucleus in the epicone and chloroplasts without a pyrenoid. This species has at least 24 latitudinal series of AVs. The characteristic features of both species were brick-like material (type E) in the eyespot and the lack of an apical furrow. These features coincide with those of Polarella glacialis, but the two species differ in cell shape, number and arrangement of AVs, shape of resting cysts, and habitats; i.e., P. glacialis has been reported only from marine cold waters. Molecular phylogeny revealed that A. miricentonis and L. pseudosanguineum were positioned in the Suessiaceae and closely related to Piscinoodinium sp., but their relationship to Polarella and other reported taxa was not supported.

  14. Molecular phylogeny of Urosomoida agilis, and new combinations: Hemiurosomoida longa gen. nov., comb. nov., and Heterourosomoida lanceolata gen. nov., comb. nov. (Ciliophora, Hypotricha).

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    Singh, Jasbir; Kamra, Komal

    2015-02-01

    For years, systematics of three species, Urosomoida agilis (Engelmann, 1862) Hemberger in Foissner, 1982, Urosomoida longa (Gelei and Szabados, 1950) Foissner et al., 1991 and Oxytricha lanceolata Shibuya, 1930, has remained unresolved due to lack of adequate molecular data. Though, it is known since several years that the three species are not very closely related. In the present paper, 18S rRNA gene sequences for two key species, U. agilis and U. longa, and their morphology and morphometry have been analyzed. Molecular phylogeny inferred from maximum likelihood, neighbour joining and maximum parsimony methods has adequately removed ambiguity over their systematics. In phylogenetic trees, U. agilis clustered consistently with non-stylonychine oxytrichids. Both Urosomoida longa and Oxytricha lanceolata clustered consistently away from U. agilis and O. granulifera, the type species of the genera Urosomoida and Oxytricha, respectively. As a result of the current molecular phylogenetic investigation and based on previously inferred morphological and morphogenetic data it is proposed to remove Urosomoida longa and Oxytricha lanceolata from Urosomoida and incertae sedis in Oxytricha, respectively, and establish two new generic combinations, Hemiurosomoida longa gen. nov., comb. nov. and Heterourosomoida lanceolata gen. nov., comb. nov. for them.

  15. Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

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    Sven eBreider

    2014-08-01

    Full Text Available Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov. and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.

  16. Crescimento de codornas de diferentes grupos genéticos por meio de modelos não lineares

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    L. F. M. Mota

    2015-10-01

    Full Text Available RESUMOObjetivou-se com o presente trabalho ajustar modelos não lineares para descrever o padrão de crescimento de genótipos de codornas de corte e de postura do nascimento aos 35 dias de idade. Foram utilizados dados de 1280 codornas machos e fêmeas, provenientes de sete genótipos de codornas de corte (EV1, EV2, UFV1, UFV2, UFV3, LF1, LF2 e um de postura. Todas as codornas foram pesadas a cada sete dias, do nascimento aos 35 dias de idade. Foram ajustados os modelos Brody, Gompertz, Logístico, Richards e von Bertalanffy aos dados de peso corporal de todos os genótipos. As análises estatísticas foram realizadas utilizando-se o PROC NLIN do SAS (Statistical Analysis System, versão 9.0. Os critérios empregados para a escolha do melhor modelo para descrever a curva de crescimento foram o coeficiente de determinação ajustado (R2, o desvio padrão assintótico (DPA, o desvio médio absoluto dos resíduos (DMA, o índice assintótico (IA, o critério de informação bayesiano (BIC, o critério de Akaike (AIC e o quadrado médio do erro (QME. Todos os modelos não lineares testados convergiram, com exceção do modelo Brody, que não convergiu para nenhum genótipo avaliado. O modelo Richards não convergiu para o genótipo postura. Os parâmetros da curva de crescimento estimados pelos modelos indicaram maior precocidade, em geral, do genótipo postura comparado aos demais genótipos. O modelo Richards apresentou superestimação do ponto de inflexão para todos os genótipos, exceto para o genótipo postura. Os modelos Gompertz, Logístico e von Bertalanffy são recomendados para descrever o crescimento de codornas de corte dos grupos genéticos em estudo. O modelo Brody não convergiu, por isso não é recomendado para descrever o crescimento dos grupos genéticos de codornas em estudo.

  17. Divergência genética entre genótipos de alface por meio de marcadores AFLP Genetics divergence among lettuce genotypes by AFLP markers

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    Cristina Soares de Sousa

    2007-01-01

    Full Text Available Considerando a restrita diversidade de espécies disponíveis para nutrir a carência de vitaminas no Brasil, Kerr e colaboradores, desde 1981, vêm desenvolvendo pesquisas para melhoramento genético de hortaliças ricas em vitamina A. Dentre elas, obtiveram uma cultivar de alface, denominada Uberlândia 10.000 com 10.200 UI de vitamina A em 100 gramas de folha fresca. Este trabalho objetivou comparar o grau de divergência genética entre a cultivar Uberlândia 10.000 e seus parentais para avaliar a eficiência da seleção utilizada, por meio da técnica AFLP. Foram utilizados os seguintes genótipos de alface: Maioba, Salad Bowl-Mimosa, Moreninha-de-Uberlândia, Vitória de Santo Antão, Uberlândia 10.000 lisa 8.ª e 9.ª geração e Uberlândia 10.000 crespa 8.ª e 9.ª geração. A técnica AFLP foi eficiente para identificar genótipos muito próximos e para estudos de progênies em alface. O primer PR15 permitiu a separação da forma lisa e crespa com 1,8% de divergência genética e a oitava da nona geração com apenas 0,71%. Com o estudo da filogenia da cultivar pode-se observar que o programa de melhoramento foi desenvolvido com sucesso, pois a cultivar obtida Uberlândia 10.000 possui alto teor de vitamina A e 92% de similaridade com o parental Vitória de Santo Antão. O primer PR11 conseguiu identificar polimorfismo entre cultivares de alta e baixa resistência à septoriose, sugerindo a possibilidade destas bandas estarem relacionadas à resistência.Considering the restricted diversity of species available to counteract vitamin deficiencies in Brazil, Kerr and coworkers have been engaged since 1981, in developing genetic improved garden vegetables rich in vitamin A. One of these vegetables is the lettuce cultivar Uberlândia 10,000, which contains 10,200 UI of vitamin A per 100 grams of fresh leaves. This study compares the genetic diversity between Uberlândia 10,000 and its parental, evaluating selection efficiency through

  18. Description of Distorhabditis poonchiana n. gen., n. sp. (Nematoda: Rhabditidae) from Jammu and Kashmir, India.

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    Shah, Ali Asghar; Vaid, Shavish; Hussain, Abid; Ahmad, Rakeeb

    2015-12-01

    Distorhabditis poonchiana n. gen., n. sp. from humus in Jammu and Kashmir, India, is described and illustrated. The new genus is characterized by a small body; slightly setoff labial region; long tubular gymnostom; prominently cuticularized cheilostom; absence of glottoid apparatus; monoprodelphic reproductive system; vulva (V) = 81 to 84; spicules with trifurcated distal ends, simple gubernaculum, peloderan bursa with eight pairs of bursal papillae arranged in 1 + 1 + 1 + 2 + 1 + 2 arrangement.

  19. A new genus, Atlanteuptychia gen. nov., for Euptychia ernestina (Lepidoptera: Nymphalidae: Satyrinae

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    André V. L. Freitas

    2013-12-01

    Full Text Available Atlanteuptychia Freitas, Barbosa & Mielke, gen. nov. is proposed for Euptychia ernestina Weymer, 1911 and illustrated. This taxon lacks the posterior projection of the tegumen, a synapomorphy of Euptychia Hübner, 1818, and does not share morphological synapomorphies with Cyllopsis R. Felder, 1869 and Paramacera Butler, 1868, two Central American genera apparently closely related to Euptychia ernestina, based on molecular data. This evidence supports the proposition of a new genus endemic to the Atlantic Forest, A. ernestina stat. nov.

  20. MicroGen: a MIAME compliant web system for microarray experiment information and workflow management

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    Pinciroli Francesco

    2005-12-01

    Full Text Available Abstract Background Improvements of bio-nano-technologies and biomolecular techniques have led to increasing production of high-throughput experimental data. Spotted cDNA microarray is one of the most diffuse technologies, used in single research laboratories and in biotechnology service facilities. Although they are routinely performed, spotted microarray experiments are complex procedures entailing several experimental steps and actors with different technical skills and roles. During an experiment, involved actors, who can also be located in a distance, need to access and share specific experiment information according to their roles. Furthermore, complete information describing all experimental steps must be orderly collected to allow subsequent correct interpretation of experimental results. Results We developed MicroGen, a web system for managing information and workflow in the production pipeline of spotted microarray experiments. It is constituted of a core multi-database system able to store all data completely characterizing different spotted microarray experiments according to the Minimum Information About Microarray Experiments (MIAME standard, and of an intuitive and user-friendly web interface able to support the collaborative work required among multidisciplinary actors and roles involved in spotted microarray experiment production. MicroGen supports six types of user roles: the researcher who designs and requests the experiment, the spotting operator, the hybridisation operator, the image processing operator, the system administrator, and the generic public user who can access the unrestricted part of the system to get information about MicroGen services. Conclusion MicroGen represents a MIAME compliant information system that enables managing workflow and supporting collaborative work in spotted microarray experiment production.