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Sample records for gen card15 presentan

  1. CARD15 gene and the classification of Crohn's disease

    NARCIS (Netherlands)

    Murillo, L; Crusius, JBA; van Bodegraven, AA; Alizadeh, BZ; Pena, AS

    2002-01-01

    An insertion mutation at nucleotide 3020 (3020insC) in the CARD15 gene, originally reported as NOD2, has been strongly associated with Crohn's disease. The CARD15 G2722C missense mutation was also shown to be associated with this disease. We studied 130 Dutch Crohn's disease patients, with a median

  2. Evidence for impaired CARD15 signalling in Crohn's disease without disease linked variants

    DEFF Research Database (Denmark)

    Seidelin, Jakob Benedict; Broom, Oliver Jay; Olsen, Jørgen

    2009-01-01

    BACKGROUND: Sensing of muramyl dipeptide (MDP) is impaired in Crohn's disease (CD) patients with disease-linked variants of the CARD15 (caspase activation and recruitment domain 15) gene. Animal studies suggest that normal CARD15 signalling prevents inflammatory bowel disease, and may be important...... for disease development in CD. However, only a small fraction of CD patients carry the disease linked CARD15 variants. The aim of this study was thus to investigate if changes could be found in CARD15 signalling in patients without disease associated CARD15 variants. METHODOLOGY/PRINCIPAL FINDINGS: By mapping...... the response to MDP in peripheral monocytes obtained from CD patients in remission not receiving immunosuppresives, an impaired response to MDP was found in patients without disease linked CARD15 variants compared to control monocytes. This impairment was accompanied by a decreased activation of IkappaB kinase...

  3. Disease duration and age influence CARD15 expression in Crohn’s disease

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    Elżbieta Poniewierka

    2016-01-01

    Full Text Available One of the susceptibility genes in Crohn’s disease (CD is CARD15. Our study examined the relationship between peripheral CARD15 expression and phenotype and duration of CD, treatment methods and inflammatory indices. Sixty patients with CD and 30 healthy volunteers as controls were enrolled in the study. Total RNA was isolated from peripheral blood mononuclear cells (PBMCs with E.Z.N.A. Total RNA Kit (Omega Bio-tek then quantitative real-time PCR was performed on the ABI Prism 7900 HT Real-Time PCR System. CARD15 gene expression in PBMCs in CD was significantly higher than in the control group. The highest level of gene expression was found in CD patients in the fourth decade of life. The mRNA level of the CARD15 gene was higher in patients with disease duration between 12 and 60 months. A positive correlation was found between erythrocyte sedimentation rate (ESR and gene expression level. Gene expression increased with increasing level of C-reactive protein and ESR, but it was not statistically significant. CARD15 expression significantly decreased in CD patients treated with anti-TNFα agents compared to azathioprine or steroid treatment groups. Expression of the CARD15 gene in Crohn›s disease is higher than in healthy individuals. Disease duration and age of patients seem to be the most important factors influencing CARD15 expression.

  4. Mutations in CARD15 and smoking confer susceptibility to Crohn's disease in the Danish population

    DEFF Research Database (Denmark)

    Ernst, Anja; Jacobsen, Bent Ascanius; Østergaard, Mette

    2007-01-01

    Three CAspase Recruitment Domain (CARD15) mutations have shown to predispose to Crohn's disease in Caucasian populations. The aim of this study was to investigate the mutation frequency in patients with inflammatory bowel disease and in healthy controls in Denmark....

  5. Role of CARD15, DLG5 and OCTN genes polymorphisms in children with inflammatory bowel diseases

    Institute of Scientific and Technical Information of China (English)

    S Cucchiara; MR Valvano; V Annese; A Latiano; O Palmieri; AM Staiano; R D'Incà; G Guariso; G Vieni; V Rutigliano; O Borrelli

    2007-01-01

    AIM: To investigate the contribution of variants of CARD15, OCTN1/2 and DLG5 genes in disease predispo sition and phenotypes in a large Italian cohort of pediatric patients with inflammatory bowel diseases (IBD).METHODS: Two hundred patients with Crohn's disease (CD), 186 ulcerative colitis (UC) patients, 434 parents (217 trios), and 347 healthy controls (HC) were studied. Polymorphisms of the three major variants of CARD15, 1672C/T and -207G/C SNPs for OCTN genes,IGR2096a_1 and IGR2198a_1 SNPs for the IBD5 locus,and 113G/A variant of the DLG5 gene were evaluated.Potential correlations with clinical sub-phenotypes were investigated.RESULTS: Polymorphisms of CARD15 were significantly associated with CD, and at least one variant was found in 38% of patients (15% in HC, OR = 2.7, P < 0.001).Homozygosis for both OCTN1/2 variants was more common in CD patients (1672TT 24%, -207CC 29%) than in HC (16% and 21%, respectively; P = 0.03), with an increased frequency of the TC haplotype (44.8% vs 38.3%in HC, P = 0.04). No association with the DLG5 variant was found. CD carriers of OCTN1/2 and DLG5 variants more frequently had penetrating disease (P = 0.04 and P = 0.01), while carriers of CARD15 more frequently had ileal localization (P = 0.03). No gene-gene interaction was found. In UC patients, the TC haplotype was more frequent (45.4%, P = 0.03), but no genotype/phenotype correlation was observed.CONCLUSION: Polymorphisms of CARD15 and OCTN genes, but not DLG5 are associated with pediatric onset of CD. Polymorphisms of CARD15, OCTN, and DLG5genes exert a weak influence on CD phenotype.

  6. NOD2/CARD15 gene mutations in patients with gouty arthritis.

    Science.gov (United States)

    Karaarslan, Ahmet; Kobak, Senol; Berdeli, Afig

    2016-11-10

    Nucleotide binding and oligomerization domains/caspase recruitment domain-containing protein 15 (NOD2/CARD15) is a cytoplasmic molecule controlling apoptosis and inflammatory processes by recognizing some microbial components. We aimed to identify the frequencies of NOD2/CARD15 gene mutations in patients with gouty arthritis and to determine their possible correlation with the disease phenotype. The study included 93 patients with gouty arthritis and 51 healthy controls matched for age, gender, and ethnicity. The NOD2/CARD15 R702W and G908R gene mutations were explored by the polymerase chain reaction restriction fragment length polymorphism method while the 3020insC mutation was analyzed by DNA sequencing. The mean patient age was 54.2 ± 14.2 years and mean duration of the disease was 3.1 ± 2.9 years. The first metatarsophalangeal and finger joint involvements were detected in 72 (77.4%) and 18 (19.5%) patients, respectively. Ankle arthritis and knee arthritis were detected in 43 (46.2%) and 20 (21.5%) patients, respectively. In total, 4 (9%) heterozygous mutations were detected in the G908R and R702W genes, while no mutation was detected in the 3020insC gene. Compared to the control group, there were no significant differences in all three DNA regions (G908R, R702W, and 3020insC; p = 0.452, p = 0.583, and p = 0.350, respectively). No correlation between the NOD2/CARD15 variants and clinical or laboratory findings (p > 0.05) was found. The frequencies of the NOD2/CARD15 gene mutations in the patients were similar to healthy control group. No association between clinical or laboratory findings and the NOD2/CARD15 gene mutations was observed.

  7. NOD2/CARD15 gene mutations in patients with gouty arthritis

    Directory of Open Access Journals (Sweden)

    Ahmet Karaarslan

    2016-11-01

    Full Text Available Nucleotide binding and oligomerization domains/caspase recruitment domain-containing protein 15 (NOD2/CARD15 is a cytoplasmic molecule controlling apoptosis and inflammatory processes by recognizing some microbial components. We aimed to identify the frequencies of NOD2/CARD15 gene mutations in patients with gouty arthritis and to determine their possible correlation with the disease phenotype. The study included 93 patients with gouty arthritis and 51 healthy controls matched for age, gender, and ethnicity. The NOD2/CARD15 R702W and G908R gene mutations were explored by the polymerase chain reaction restriction fragment length polymorphism method while the 3020insC mutation was analyzed by DNA sequencing. The mean patient age was 54.2 ± 14.2 years and mean duration of the disease was 3.1 ± 2.9 years. The first metatarsophalangeal and finger joint involvements were detected in 72 (77.4% and 18 (19.5% patients, respectively. Ankle arthritis and knee arthritis were detected in 43 (46.2% and 20 (21.5% patients, respectively. In total, 4 (9% heterozygous mutations were detected in the G908R and R702W genes, while no mutation was detected in the 3020insC gene. Compared to the control group, there were no significant differences in all three DNA regions (G908R, R702W, and 3020insC; p = 0.452, p = 0.583, and p = 0.350, respectively. No correlation between the NOD2/CARD15 variants and clinical or laboratory findings (p > 0.05 was found. The frequencies of the NOD2/CARD15 gene mutations in the patients were similar to healthy control group. No association between clinical or laboratory findings and the NOD2/CARD15 gene mutations was observed.

  8. Penetrance of NOD2/CARD15 genetic variants in the general population

    DEFF Research Database (Denmark)

    Yazdanyar, Shiva; Kamstrup, Pia R; Tybjaerg-Hansen, Anne;

    2010-01-01

    In case-control studies of Europeans, heterozygosity for Arg702Trp(rs2066844), Gly908Arg(rs2066845) and Leu1007fsinsC(rs5743293) on the NOD2/CARD15 gene is associated with a 2-fold greater risk of Crohn disease, whereas homozygosity or compound heterozygosity is associated with a 17-fold greater ...

  9. NOD2/CARD15 gene polymorphism in patients with inflammatory bowel disease: Is Hungary different?

    Institute of Scientific and Technical Information of China (English)

    Carsten Büning; Tomas Mlolnar; Ferenc Nagy; Janos Lonovics; Renita Weltrich; Bettina Bochow; Janine Genschel; Hartmut Schmidt; Herbert Lochs

    2005-01-01

    AIM: To analyse the impact of NOD2/CARD15 mutations on the clinical course of Crohn's disease patients from an eastern European country (Hungary).METHODS: We investigated the prevalence of the three common NOD2/CARD15 mutations (Arg702Trp, Gly908Arg,1007finsC) in 148 patients with Crohn's disease, 128patients with ulcerative colitis and 208 controls recruited from the University of Szeged, Hungary. In patients with Crohn's disease, the prevalence of NOD2/CARD15 mutations was correlated to the demographical and clinical parameters.RESULTS: In total, 32.4% of Crohn's disease patients carried at least one mutant allele within NOD2/CARD15compared to 13.2% of patients with ulcerative colitis (P = 0.0002) and to 11.5% of controls (P<0.0001). In Crohn's disease patients, the allele frequencies for Arg702Trp,Gly908Arg and 1007finsC were 7.1%, 3.0% and 10.8%respectively. Interestingly, only the 1007finsC mutation was associated with a distinct clinical phenotype. The patients positive for the 1007finsC mutation suffered more frequently from stenotic disease behaviour (P = 0.008). Furthermore,51.9% of patients positive for the 1007finsC mutation underwent a surgical resection within the ileum compared to only 17.4% of patients without the 1007finsC mutation (P = 0.001). With respect to the other two mutations (Arg702Trp and Gly908Arg), no associations were found with all investigated clinical parameters.CONCLUSION: NOD2/CARD15 mutations are frequently found in Crohn's disease patients from Hungary. The 1007finsC mutation is associated with stenotic disease behaviour and frequent ileal resections.

  10. Incidence of Crohn's disease and CARD15 mutation in a small township in Sicily.

    Science.gov (United States)

    Cottone, M; Renda, M C; Mattaliano, A; Oliva, L; Fries, W; Criscuoli, V; Modesto, I; Scimeca, D; Maggio, A; Casà, A; Maisano, S; Mocciaro, F; Sferrazza, A; Orlando, A

    2006-01-01

    The incidence of Crohn's disease (CD) has been shown to be lower in Southern than in Northern Europe. Data on the frequency of the NOD2/CARD15 mutations for Mediterranean area are very scant. To determine the incidence of CD from 1979 to 2002 in a township in Sicily together with the allele frequency of NOD2/CARD15 mutations in patients, family members and controls, and to determine the allele frequency of these mutations in sporadic CD from other areas of Sicily in comparison with a control population. Casteltermini is a small town close to Agrigento (Sicily) with a population of 9,130 inhabitants. All the diagnoses of inflammatory bowel disease (IBD) made from 1979 to 2002 were obtained through the local health authority. NOD2/CARD15 mutations were studied in 23 out of the 29 patients with CD in Casteltermini, in 60 family members and in 64 controls. NOD2/CARD15 was also studied in 80 sporadic cases of CD disease among Sicilians outside Casteltermini and 118 healthy controls. From 1979 to 2002, 29 patients with CD and 13 patients with ulcerative colitis (UC) were registered. The 6-year mean incidence of CD ranged from 8.0 to 17 new cases for every 100,000 inhabitants, whereas the mean incidence of UC ranged from five new cases to 7.8 for every 100,000 inhabitants. The allele frequencies of NOD2/CARD15 mutations (L1007finsC, G908R, R702W) were 8.7, 4.3 and 8.7%, respectively, in CD cases; 5.0, 4.2 and 3.1% in family members; 1.6, 2.3 and 3.1% in controls. In sporadic Sicilian CD patients outside Casteltermini the allele frequency was 7.5, 8.1, 6.2% whereas in control population it was 3.3, 1.6, 1.6%. A high incidence of CD compared with UC was observed in this small town in Southern Italy. The frequency of NOD2/CARD15 mutations in CD is similar to other Caucasian population studied so far.

  11. MEFV, TNF1rA, CARD15 and NLRP3 mutation analysis in PFAPA.

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    Dagan, Efrat; Gershoni-Baruch, Ruth; Khatib, Ihab; Mori, Adi; Brik, Riva

    2010-03-01

    PFAPA is a periodic fever disease, of unknown etiology, characterized by aphthous stomatitis, pharyngitis and cervical adenitis. To inquire whether genes implicated in other auto-inflammatory diseases might be involved in its pathogenesis, predominant mutations in the genes causing familial Mediterranean fever, TNF receptor-associated periodic fever syndrome, Crohn's disease and Muckel-Wells syndrome were analyzed in PFAPA patients. Patients (n = 57) with PFAPA, according to previously published criteria were recruited, at the Meyer Children Hospital during 2006-2007. Clinical information was complemented during physicians-parents encounter. Predominant mutations in MEFV, TNF1rA, CARD15/NOD2 and NLRP3 genes were tested. Mean age at diagnosis was 30.64 +/- 16.4 months. Boys (n = 33; 58%) were diagnosed earlier than girls (n = 21; 42%) at 26.18 +/- 13.83 and 36.41 +/- 18.32 months, respectively (P = 0.05). Fifteen patients (27%) carried an MEFV mutation; two patients (3.6%) a CARD15 mutation, one patient (1.8%) a variance in TNF1rA and another had both an MEFV and a CARD15 mutation. Clinical symptoms were equally manifested in carriers and non-carriers. The high carrier rate of MEFV mutations in our PFAPA cases compares well with that of the general population in Israel. It is debated whether MEFV mutations, when mediated by the presence of additional modifiers, may expose a transient fever condition, namely PFAPA.

  12. OCTN and CARD15 gene polymorphism in Chinese patients with inflammatory bowel disease

    Institute of Scientific and Technical Information of China (English)

    Mei Li; Xiang Gao; Chang-Cun Guo; Kai-Chun Wu; Xin Zhang; Pin-Jin Hu

    2008-01-01

    AIM:To investigate the single nucleotide polymorphism(SNPs)distribution of NOD2/CARD15(R702W,G908R),OCTN1 1672C/T and OCTN2-207G/C in Chinese patients with inflammatory bowel disease(IBD).METHODS:A total of 61 patients with Crohn's disease(CD),151 patients with ulcerative colitis(UC),and 200 unrelated healthy controls were genotyped.Genotyping was performed by sequence specific primer polymerase chain reaction(PCR-SSP)or by restriction fragment length polymorphism(PCR-RFLP)analysis.RESULTS:Among the subjects in our study groups,including patients with CD,UC and healthy controls,none had OCTN and CARD15 variants and very rare IBD family history was found in our patients with the percentage of 0(0/61 with CD)and 1.3%(2/151 with UC).CONCLUSION:Our results indicate that although OCTN or CARD15 variation is associated with susceptibility to IBD in Western populations,these might be rare and may not be associated with susceptibility to IBD in Chinese patients.

  13. Penetrance of NOD2/CARD15 genetic variants in the general population

    DEFF Research Database (Denmark)

    Yazdanyar, Shiva; Kamstrup, Pia R; Tybjaerg-Hansen, Anne

    2010-01-01

    In case-control studies of Europeans, heterozygosity for Arg702Trp(rs2066844), Gly908Arg(rs2066845) and Leu1007fsinsC(rs5743293) on the NOD2/CARD15 gene is associated with a 2-fold greater risk of Crohn disease, whereas homozygosity or compound heterozygosity is associated with a 17-fold greater ...... risk. However, the importance of these genetic variants if identified in particular individuals within the general population is unknown. We undertook this study to estimate the penetrance of these variants in the general population....

  14. Análisis del gen CARD15 en uveítis idiopática

    OpenAIRE

    Rodríguez Pérez, Noelia

    2011-01-01

    Nod2 es un receptor de reconocimiento de patrones (PRR) citoplásmico, perteneciente a la familia de receptores de tipo NOD (NLR) y consta de tres tipos de dominios estructurales y funcionales distintos: dos dominios de reclutamiento de caspasas (CARD) en el extremo amino-terminal, un dominio central de unión a nucleótidos (NBD) y un dominio de repeticiones ricas en leucina (LRR) en el extremo carboxi-terminal. Este último, permite el reconocimiento de muramil dipéptido (producto resultante de...

  15. Análisis del gen CARD15 en uveítis idiopática

    OpenAIRE

    Rodríguez Pérez, Noelia

    2009-01-01

    Nod2 es un receptor de reconocimiento de patrones (PRR) citoplásmico, perteneciente a la familia de receptores de tipo NOD (NLR) y consta de tres tipos de dominios estructurales y funcionales distintos: dos dominios de reclutamiento de caspasas (CARD) en el extremo amino-terminal, un dominio central de unión a nucleótidos (NBD) y un dominio de repeticiones ricas en leucina (LRR) en el extremo carboxi-terminal. Este último, permite el reconocimiento de muramil dipéptido (producto resultante de...

  16. Microbial induction of CARD15 expression in intestinal epithelial cells via toll-like receptor 5 triggers an antibacterial response loop.

    Science.gov (United States)

    Begue, B; Dumant, C; Bambou, J C; Beaulieu, J F; Chamaillard, M; Hugot, J P; Goulet, O; Schmitz, J; Philpott, D J; Cerf-Bensussan, N; Ruemmele, F M

    2006-11-01

    With the discovery of CARD15 as susceptibility gene for Crohn's disease (CD) a first link to a potential defect in the innate immune system was made. In this work we aimed to analyze enterocyte NOD2/CARD15 expression and regulation in response to bacterial motifs and the consequences of the most common CD-specific CARD15 mutation on antibacterial responses of normal intestinal epithelial cells (IEC). Under normal conditions, IEC lines and ileal enterocytes did not express NOD2/CARD15 mRNA or protein, contrary to IEC derived from inflammatory CD sections. In vitro analyses revealed that the simple contact with non-pathogenic commensal E. Coli K12 was sufficient to induced NOD2/CARD15 mRNA and protein in human IEC (HIEC). We identified bacterial flagellin interacting with TLR5 as major motif in this regulation of NOD2/CARD15. E. Coli mutants not expressing flagellin (DeltaFliC) failed to induce CARD15. Similarly, in HIEC transfected with a plasmid encoding dominant negative TLR5, no CARD15 induction was observed after K12 contact. Isolated TLR2 or TLR4 stimulation had no or only a marginal effect on NOD2/CARD15 expression. NOD2/CARD15 negative HIEC were unresponsive to muramyl dipeptide (MDP), but once NOD2/CARD15 was induced, HIEC and Caco2 cells responded to intra or extracellular MDP presentation with the activation of the NFkB pathway. IEC transfected with the Crohn-specific CARD15 mutant (F3020insC, FS) failed to activate NFkB after MDP-challenge, in contrast to CARD15WT IEC. In response to MDP, IEC induced a massive antibacterial peptide (ABP) response, seen in the apical release of CCL20. This was completely abolished in IEC carrying CARD15FS. These data suggest a critical role of NOD2/CARD15 in the bacterial clearance of the intestinal epithelium while CD-specific mutated NOD2/CARD15 causes an impaired epithelial barrier.

  17. Associations between NOD2/CARD15 genotype and phenotype in Crohn's disease-Are we there yet?

    Institute of Scientific and Technical Information of China (English)

    Graham Radford-Smith; Nirmala Pandeya

    2006-01-01

    There have been multiple NOD2/CARD15 genotypephenotype analyses undertaken in patients with Crohn's disease since the gene's discovery in 2001. This review focuses on the major published series based upon their size and on the presence of specific clinical and genetic information provided in the published material from 2001 to 2005. Twelve studies provided raw data to carry out comparisons of disease location while ten studies included analysis of NOD2/CARD15 genotypes.NOD2/CARD15 variant frequency in ileal disease did not differ significantly among studies, whereas a comparison of disease location demonstrated highly significant differences among studies. Meta-analysis confirmed significant associations between NOD2/CARD15variants and both ileal and ileocolonic disease locations,and with both stricturing and penetrating forms of disease behavior. This review underlines the significant phenotypic differences that exist among populations,including similar ethnic groups, and has demonstrated the need for further studies of patients with long-term "inflammatory" Crohn's disease.

  18. CARD15 gene overexpression reduces effect of etanercept, infliximab, and adalimumab on cytokine secretion from PMA activated U937 cells.

    Science.gov (United States)

    Teimourian, Shahram; Masoudzadeh, Nooshin

    2015-09-05

    Crohn's disease (CD), a subcategory of inflammatory bowel disease, is an immune-related disorder characterized by inflammation of the gastrointestinal mucosa, which can take place in any region along the alimentary tract. The most important gene involved in the etiology of CD is NOD2/CARD15 located on chromosome 16. It has been shown that CARD15 is overexpressed in monocytes of CD patients. The common treatment for the disease is anti-TNF-alpha drugs, the most hopeful of which are probably infliximab and etanercept. Infliximab rapidly reduces signs and symptoms of active Crohn's disease. In contrast, etanercept shows no such effect. In the present study, we evaluated the effects of the CARD15 gene overexpression in monocytic cell line U937 in the production of anti-inflammatory cytokine, IL-10, and proinflammatory cytokine, Il-1 beta, produced after incubation with infliximab, adalimumab, and etanercept separately. Our results show that infliximab and adalimumab significantly decreased IL-10 and IL-1beta secretion levels. However, etanercept inhibition of secretion was less compared with infliximab or adalimumab. In all three cases, suppression of cytokine production is reduced by CARD15 overexpression.

  19. CARD15 Gene 3020insC Mutation with Inflammatory Bowel Diseases Patients in the Black Sea Region of Turkey

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    Ilhami Gok

    2014-06-01

    CONCLUSION: This study is to investigate a relation between CARD15/NOD2 3020insC frameshift mutation and in patients with IBD in the Turkish Population. C-insertion frameshift mutation is a major contributor to the susceptibility to both CD and UC, but it is not specific to patients with CD in Turkish population.

  20. Blau Syndrome-Related CARD15/NOD2 Mutations Are Not Linked to Idiopathic Uveitis in Spanish Patients

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    Noelia Rodríguez-Pérez

    2009-01-01

    Full Text Available Uveitis is a clinical feature of the Blau syndrome, a disease linked to CARD15 (also referred to as NOD2 mutations. Three main mutations in this gene (R334W, R334Q and L469F have been reported as Blau syndrome risk factors, a disease that manifests uveitis as one of its clinical features. However, little is known on the involvement of this gene in idiopathic uveitis. We thus sought to determine the frequency of these Blau-related CARD15 mutations in a cohort of Spanish patients with idiopathic uveitis. To this aim, 110 patients with idiopathic uveitis, followed at the Department of Ophtalmology of a tertiary hospital (Hospital Universitario Alcalá de Henares, Madrid. Spain were enrolled. As a control population, 104 healthy subjects were used. DNA was extracted from blood samples and the Blau-related CARD15 mutations were analysed either by PCR-RFLP or direct DNA sequencing. None of the mutations studied was found in any of the individuals tested, whether diseased or healthy. It seems thus that Blau syndrome-related CARD15 mutations are not involved in idiopathic uveitis, a finding which allows us to suggest that the genetic aetiology of the idiopathic uveitis or the Blau-associated uveitis is different.

  1. NOD2/CARD15 genotype, cardiovascular disease and cancer in 43 600 individuals from the general population

    DEFF Research Database (Denmark)

    Yazdanyar, S.; Nordestgaard, B.G.

    2010-01-01

    from two large Danish general population cohorts followed for 31 years: the Copenhagen City Heart Study (n = 10 597) and the Copenhagen General Population Study (n = 32 999). We examined the risk of cardiovascular disease (2743 and 3890, respectively, in the two studies) and cancer (2144 and 3241...... the general population. J Intern Med 2010; 268: 162-170. Objectives. The NOD2/CARD15 gene is involved in the innate immune response, and thus in inflammation. Three polymorphisms in this gene (Arg702Trp rs2066844, Gly908Arg rs2066845 and Leu1007fsinsC rs5743293) have been associated with increased risk...... variants pooled with compound heterozygotes for two variants. Results. Multifactorially adjusted hazard ratios for cardiovascular disease and cancer in NOD2/CARD15 heterozygotes or homozygotes/compound heterozygotes versus noncarries did not differ from 1.0 in the Copenhagen City Heart Study...

  2. NOD2/CARD15 genotype, cardiovascular disease and cancer in 43,600 individuals from the general population

    DEFF Research Database (Denmark)

    Yazdanyar, S; Nordestgaard, B G

    2010-01-01

    The NOD2/CARD15 gene is involved in the innate immune response, and thus in inflammation. Three polymorphisms in this gene (Arg702Trp rs2066844, Gly908Arg rs2066845 and Leu1007fsinsC rs5743293) have been associated with increased risk of the inflammatory bowel disease, the Crohn's disease. We...... tested whether these polymorphisms are also associated with increased risk of cardiovascular disease and cancer, in which the innate immune system and inflammation may influence pathogenesis....

  3. NOD2/CARD15 Gene Polymorphisms in Crohn's Disease: A Genotype-Phenotype Analysis in Danish and Portuguese Patients and Controls

    DEFF Research Database (Denmark)

    Vind, Ida; Vieira, A; Hougs, L

    2005-01-01

    BACKGROUND: A North-South gradient in Crohn's disease (CD) implying a higher incidence in northern Europe compared to southern Europe has been established. AIMS: To investigate whether there is a difference between Denmark and Portugal in the frequency of CARD15 mutations in CD patients compared ...... a trend towards more mutations in the Portuguese controls was seen, a relationship between CD and CARD15 mutations was observed in both countries. Copyright (c) 2005 S. Karger AG, Basel....

  4. Common NOD2/CARD15 variants are not associated with susceptibility or the clinicopathologic characteristics of sporadic colorectal cancer in Hungarian patients

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    Gemela Orsolya

    2007-03-01

    Full Text Available Abstract Background Epidemiological observations suggest that cancer arises from chronically inflamed tissues. Inflammatory bowel disease (IBD is a typical example as patients with longstanding IBD are at an increased risk for developing colorectal cancer (CRC and mutations of the NOD2/CARD15 gene increase the risk for Crohn's disease (CD. Recently, NOD2/CARD15 has been associated with a risk for CRC in some studies, which stemmed from ethnically diverse populations. Our aim was to identify common NOD2/CARD15 mutations in Hungarian patients with sporadic CRC. Methods A total of 194 sporadic CRC patients (m/f: 108/86, age at diagnosis of CRC: 63.2 ± 9.1 years old and 200 healthy subjects were included. DNA was screened for SNP8, SNP12 and SNP13 NOD2/CARD15 mutations by denaturing-HPLC and confirmed by direct sequencing. Results NOD2/CARD15 mutations were found in 28 patients (14.4% and in 23 controls (11.5%, p = NS. Allele frequencies for SNP8/R702W (1.8% vs. 1.5% SNP12/G908R (1.8% vs. 1.8% and SNP13/3020insC (3.6% vs. 2.5% were also not statistically different between patients and controls. The clinicopathologic characteristics of CRC patients with or without NOD2/CARD15 mutations were not significantly different. Conclusion Our results suggest that common NOD2/CARD15 mutations alone do not contribute to CRC risk in the Hungarian population.

  5. CARD15 single nucleotide polymorphisms 8, 12 and 13 are not increased in ethnic Danes with sarcoidosis

    DEFF Research Database (Denmark)

    Milman, Nils; Nielsen, Ole Haagen; Hviid, Thomas Vauvert F

    2007-01-01

    and SNP13, respectively, were performed by capillary electrophoresis single-strand confirmation polymorphism in 53 patients with histologically verified sarcoidosis and in 103 healthy controls. RESULTS: The frequencies of CARD15 mutations in sarcoidosis patients were: SNP8, 4/106 chromosomes (3.8%); SNP12......, 2/106 chromosomes (1.9%); SNP13, 2/106 chromosomes (1.9%); SNP8+SNP12+SNP13, 8/106 chromosomes (7.6%). All 8 patients were heterozygous. The frequencies in controls were: SNP8, 9/206 chromosomes (4.4%); SNP12, 2/206 chromosomes (1.0%); SNP13, 4/206 chromosomes (1.9%); SNP8+SNP12+SNP13, 15...

  6. Toll-like receptor 4 and NOD2/CARD15 mutations in Hungarian patients with Crohn's disease: Phenotype-genotype correlations

    Institute of Scientific and Technical Information of China (English)

    Peter Laszlo Lakatos; Gyula Mozsik; Hungarian IBD Study Group; Peter Ferenci; Laszlo Lakatos; Ferenc Szalay; Claudia Willheim-Polli; Christoph (O)sterreicher; Zsolt Tulassay; Tamas Molnar; Walter Reinisch; Janos Papp

    2005-01-01

    AIM: To determine common NOD2/CARD15 mutations and TLR4 D299G polymorphism in Hungarian patients with CD.METHODS: A total of 527 unrelated patients with CD (male/female: 265/262, age: 37.1 (SD 7.6) years) and 200 healthy subjects were included. DNA was screened for possible NOD2/CARD15 mutations by denaturing highperformance liquid chromatography (confirmed by direct sequencing). TLR4 D299G was tested by PCR-RFLP.RESULTS: NOD2/CARD15 mutations were found in 185patients (35.1%) and in 33 controls (16.5%, P<0.0001).SNP8/R702W (10.8% vs 6%, P = 0.02), SNP13/3020insC (19.4% vs 5%, P<0.0001) and exon4 R703C (2.1% vs 0%, P = 0.02) mutations were more frequent in CD, while the frequency of SNP12/G908R was not increased. The frequency of TLR4 D299G was not different (CD: 9.9% vscontrols: 12.0%). Variant NOD2/CARD15 allele was associated with an increased risk for CD (ORhet = 1.71,95%CI = 1.12-2.6, P= 0.0001, ORtwo-riskalleles = 25.2,95%CI = 4.37- , P<0.0001), early disease onset (carrier:26.4 years vs non-carrier: 29.8 years, P = 0.0006), ileal disease (81.9% vs 69.5%, OR = 1.99, 95%CI = 1.29-3.08,P = 0.02, presence of NOD2/CARD15 and TLR4: 86.7% vs64.8%), stricturing behavior (OR = 1.69, 95%CI = 1.13-2.55,P = 0.026) and increased need for resection (OR=1.71,95%CI: 1.13-2.62, P= 0.01), but not with duration, extraintestinal manifestations, familial disease or smoking. TLR4exhibited a modifier effect: age of onset in wt/TLR4 D299G carriers: 27.4 years vs NOD2mut/TLR D299G: 23 years (P= 0.06), in NOD2mut/wt: 26.7 years.CONCLUSION: These results confirm that variant NOD2/CARD15 (R702W, R703C and 3020insC) alleles are associated with earlier disease onset, ileal disease,stricturing disease behavior in Hungarian CD patients. In contrast, although the frequency of TLR4 D299G polymorphism was not different from controls, NOD2/TLR4mutation carriers tended to present at earlier age.

  7. Favourable effect of TNF-alpha inhibitor (infliximab) on Blau syndrome in monozygotic twins with a de novo CARD15 mutation

    DEFF Research Database (Denmark)

    Milman, Nils; Andersen, Claus B.; Hansen, Annette

    2006-01-01

    Blau syndrome is a hereditary granulomatous disease caused by mutations in the CARD15 gene that is diagnosed in children of young age with exanthema/erythema, arthritis/periarthritis and/or uveitis. We report two cases of Blau syndrome in Danish Caucasian monozygotic male twins, exhibiting...... a heterozygous de novo R334W mutation in codon 334 of CARD15. The patients were initially diagnosed as having sarcoidosis. In both twins, symptoms (exanthema, arthritis/periarthritis) started at 1 year of age, and were followed by uveitis at 7-10 years of age. There was no involvement of the lungs or other...... quality of life. At follow up at 20 years of age (after 2-5 years of infliximab treatment) the twins had an almost normal physical appearance and a normal psychomotoric development, indicating a favourable short-term prognosis of the disease. Blau syndrome has pathologic, clinical and therapeutic features...

  8. NOD2/CARD15 , ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn’s disease

    Institute of Scientific and Technical Information of China (English)

    Maria; Gazouli; Ioanna; Pachoula; Ioanna; Panayotou; Gerassimos; Mantzaris; George; Chrousos; Nicholas; P; Anagnou; Eleftheria; Roma-Giannikou

    2010-01-01

    AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in adult-onset Crohn’s disease (CD). METHODS: Polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 ; rs2241880 A/G of ATG16L1 , and rs11209026 (R381Q) of IL23R gene were assessed in 110 childhood-onset CD, 364 adult-onset CD, and 539 healthy individuals. Analysis of polymorphisms R702W, G908R, ...

  9. Prevalence of SLC22A4, SLC22A5 and CARD15 gene mutations in Hungarian pediatric patients with Crohn's disease

    Institute of Scientific and Technical Information of China (English)

    Judit Bene; Lili Magyari; Gábor Talián; Katalin Komlósi; Beáta Gasztonyi; Beáta Tari; (A)gnes Várkonyi; Gyula Mózsik; Béla Melegh

    2006-01-01

    AIM: To investigate the frequency of the common NOD2/CARD15 susceptibility variants and two functional polymorphisms of OCTN cation transporter genes inHungarian pediatric patients with Crohn's disease (CD).METHODS: A cohort of 19 unrelated pediatric and 55 unrelated adult patients with Crohn's disease and 49 healthy controls were studied. Genotyping of the threecommon CD-associated CARD15 variants (Arg702Trp, Gly908Arg and 1007finsC changes) with the SLC22A4 1672C→T, and SLC22A5 -207G→C mutations was performed by direct sequencing of the specific regions of these genes.RESULTS: At least one CARD15 mutation was present in 52.6% of the children and in 34.5% of the adults compared to 14.3% in controls. Surprisingly, strongly different mutation profile was detected in the pediatric versus adult patients. While the G908R and 1007finsC variants were 18.4% and 21.1% in the pediatric group, they were 1.82% and 11.8% in the adults, and were 1.02% and 3.06% in the controls, respectively. The R702W allele was increased approximately two-fold in the adult subjects, while in the pediatric group it was only approximately 64% of the controls (9.09% in the adults, 2.63% in pediatric patients, and 4.08% in the controls). No accumulation of the OCTN variants was observed in any patient group versus the controls. CONCLUSION: The frequency of the NOD2/CARD15susceptibility variants in the Hungarian pediatric CD population is high and the profile differs from the adult CD patients, whereas the results for SLC22A4 and SLC22A5 mutation screening do not confirm the assumption that the carriage of these genotypes means an obligatory susceptibility to CD.

  10. Polymorphisms in interleukin-10 gene according to mutations of NOD2/CARD15 gene and relation to phenotype in Spanish patients with Crohn's disease

    Institute of Scientific and Technical Information of China (English)

    Juan L Mendoza; Elena Urcelay; Raquel Lana; Alfonso Martinez; Carlos Taxonera; Emilio G de la Concha; Manuel Díaz-Rubio

    2006-01-01

    AIM: To examine the contribution of interleukin-10(IL-10) gene polymorphisms to Crohn's disease (CD)phenotype, and the possible genetic epistasis between IL-10 gene polymorphisms and CARD15/NOD2 gene mutations.METHODS: A cohort of 205 Spanish unrelated patients with Crohn's disease recruited from a single center was studied. All patients were rigorously phenotyped and followed-up for at least 3 years (mean time, 12.5years). The clinical phenotype was established prior to genotyping.RESULTS: The correlation of genotype-Vienna classification groups showed that the ileocolonic location was significantly associated with the -1082G allele in the NOD2/CARD15 mutation-positive patients (RR= 1.52,95%CI, 1.21 to 1.91,P= 0.008). The multivariate analysis demonstrated that the IL-10 G14 microsatellite allele in the NOD2/CARD15 mutation positive patients was associated with two risk factors, history of appendectomy(RR=2.15, 95%CI=1.1-4.30, P=0.001) and smoking habit at diagnosis (RR = 1.29, 95%CI= 1.04-4.3,P= 0.04).CONCLUSION: In Spanish population from Madrid, in CD patients carrying at least one NOD2/CARD15 mutation,the -1082G allele is associated with ileocolonic disease and the IL-10G14 microsatellite allele is associated with previous history of appendectomy and smoking habit at diagnosis. These data provide further molecular evidence for a genetic basis of the clinical heterogeneity of CD.

  11. Favourable effect of TNF-alpha inhibitor (infliximab) on Blau syndrome in monozygotic twins with a de novo CARD15 mutation

    DEFF Research Database (Denmark)

    Milman, Nils; Andersen, Claus B; Hansen, Annette

    2006-01-01

    quality of life. At follow up at 20 years of age (after 2-5 years of infliximab treatment) the twins had an almost normal physical appearance and a normal psychomotoric development, indicating a favourable short-term prognosis of the disease. Blau syndrome has pathologic, clinical and therapeutic features...... in common with sarcoidosis, but rarely involves the lungs or other parenchymatous organs. In children, discrimination between early onset sarcoidosis and Blau syndrome should include a CARD15 mutation analysis....

  12. NOD2/CARD15 mutations in Polish and Bosnian populations with and without Crohn’s disease: prevalence and genotype-phenotype analysis

    Science.gov (United States)

    Salkic, Nermin N.; Adler, Grazyna; Zawada, Iwona; Alibegovic, Ervin; Karakiewicz, Beata; Kozlowska-Wiechowska, Anna; Wasilewicz, Michał; Sulzyc-Bielicka, Violetta; Bielicki, Dariusz

    2015-01-01

    Data on prevalence and phenotypic consequences of nucleotide-binding oligomerisation domain 2/caspase recruitment domains 15 (NOD2/CARD15) variants in Crohn’s disease (CD) population in Poland and Bosnia and Herzegovina (B&H) are nonexistent. We aimed to determine the prevalence of NOD2/CARD15 mutations and their association with disease phenotype in Polish and Bosnian patients with CD and in healthy controls. We prospectively recruited 86 CD patients and 83 controls in Poland and 30 CD patients and 30 controls in B&H, 229 in total. We determined the prevalence of NOD2/CARD15 mutations and their association with the disease phenotype according to Montreal classification. Participants were genotyped for Leu1007fsinsC and Gly908Arg mutations. At least one CD-associated allele was found in 29/86 (33.7%) of Polish CD patients and in 9/83 (10.8%) of healthy controls (p<0.001). In both CD patients and controls in Bosnian sample, at least one NOD2 mutation was found in equal number of patients (3/30; 10%) with all of the NOD2 mutation positive CD patients being homozygous, while controls being heterozygous. In Polish sample, perianal disease was less frequent in CD patients with any NOD2 mutation (1/21; 4.8%) compared to those without (11/41; 26.8%; p=0.046). Higher percentage of patients with NOD2 mutations had history of CD related surgery when compared with those without mutations (66.7% vs. 43.3%; p=0.05). The risk for CD is increased in patients with NOD2 mutations (Poland) and especially in the presence of homozygous NOD2 mutations (Poland and Bosnia). The presence of variant NOD2 alleles is associated with increased need for surgery and reduced occurrence of perianal disease. PMID:26042516

  13. NOD2/CARD15 mutations in Polish and Bosnian populations with and without Crohn's disease: prevalence and genotype-phenotype analysis

    Directory of Open Access Journals (Sweden)

    Nermin N Salkic

    2015-05-01

    Full Text Available Data on prevalence and phenotypic consequences of nucleotide-binding oligomerisation domain 2/caspase recruitment domains 15 (NOD2/CARD15 variants in Crohn's disease (CD population in Poland and Bosnia and Herzegovina (B&H are nonexistent. We aimed to determine the prevalence of NOD2/CARD15 mutations and their association with disease phenotype in Polish and Bosnian patients with CD and in healthy controls. We prospectively recruited 86 CD patients and 83 controls in Poland and 30 CD patients and 30 controls in B&H, 229 in total. We determined the prevalence of NOD2/CARD15 mutations and their association with the disease phenotype according to Montreal classification. Participants were genotyped for Leu1007fsinsC and Gly908Arg mutations. At least one CD-associated allele was found in 29/86 (33.7% of Polish CD patients and in 9/83 (10.8% of healthy controls (p<0.001. In both CD patients and controls in Bosnian sample, at least one NOD2 mutation was found in equal number of patients (3/30; 10% with all of the NOD2 mutation positive CD patients being homozygous, while controls being heterozygous. In Polish sample, perianal disease was less frequent in CD patients with any NOD2 mutation (1/21; 4.8% compared to those without (11/41; 26.8%; p=0.046. Higher percentage of patients with NOD2 mutations had history of CD related surgery when compared with those without mutations (66.7% vs. 43.3%; p=0.05. The risk for CD is increased in patients with NOD2 mutations (Poland and especially in the presence of homozygous NOD2 mutations (Poland and Bosnia. The presence of variant NOD2 alleles is associated with increased need for surgery and reduced occurrence of perianal disease.

  14. Clinical significance of NOD2/CARD15 and Toll-like receptor 4 gene single nucleotide polymorphisms in inflammatory bowel disease

    Institute of Scientific and Technical Information of China (English)

    Luciana Rigoli; Maria D Sergi; Caterina Cuppari; Giovanna Elisa Calabrò; Romina Gallizzi; Carmelo Damiano Salpietro; Walter Fries; Claudio Romano; Rosario Alberto Caruso; Maria A Lo Presti; Chiara Di Bella; Vincenzo Procopio; Giuseppina Lo Giudice; Maria Amorini; Giuseppe Costantino

    2008-01-01

    AIM: To evaluate the role of genetic factors in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC), we investigated the single nucleotide poly-morphisms (SNPs) of NOD21CARD15 (R702W, G908R and L1007finsC), and Toll-like receptor 4 (TLR4) genes (D299G and T3991) in a selected inflammatory bowel disease (IBD) population coming from Southern Italy.METHODS: Allele and genotype frequencies of NOD2/CARD15 (R702W, G908R and L1007finsC) and TLR4 (D299G and T3991) SNPs were examined in 133 CD pa-tients, in 45 UC patients, and in 103 healthy controls. A genotype-phenotype correlation was performed.RESULTS: NOD2/CARD15 R702W mutation was sig-nificantly more frequent in CD (9.8%) than in controls(2.4%,P=0.001) and in UC (2.3%,P=0.03). No sig-nificant difference was found between UC patients and control group (P>0.05). In CD and UC patients, no significant association with G908R variant was found.L1007finsC SNP showed an association with CD (9.8%)compared with controls (2.9%,P=0.002) and UC patients (2.3%,P=0.01). Moreover, in CD patients,G908R and L1007finsC mutations were significantly associated with different phenotypes compared to CD wild-type patients. No association of IBD with the TLR4 SNPs was found in either cohort (allele frequencies:D299G-controls 3.9%, CD 3.7%, UC 3.4%, P>0.05;T399I-controls 2.9%, CD 3.0%, UC 3.4%,P>0.05).CONCLUSION: These findings confirm that, in our IBD patients selected from Southern Italy, the NOD2/CARD15, but not TLR4 SNPs, are associated with in-creased risk of CD.

  15. GEN 480 uop / uophelp

    OpenAIRE

    2015-01-01

    GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEM 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment Paper and Matrix GEN 480 Week 2 DQ 1 GEN 480 Week 2 DQ 2 GEN 480 Week 2 DQ 3 ...

  16. Direct and indirect induction by 1,25-dihydroxyvitamin D3 of the NOD2/CARD15-defensin beta2 innate immune pathway defective in Crohn disease.

    Science.gov (United States)

    Wang, Tian-Tian; Dabbas, Basel; Laperriere, David; Bitton, Ari J; Soualhine, Hafid; Tavera-Mendoza, Luz E; Dionne, Serge; Servant, Marc J; Bitton, Alain; Seidman, Ernest G; Mader, Sylvie; Behr, Marcel A; White, John H

    2010-01-22

    Vitamin D signaling through its nuclear vitamin D receptor has emerged as a key regulator of innate immunity in humans. Here we show that hormonal vitamin D, 1,25-dihydroxyvitamin D(3), robustly stimulates expression of pattern recognition receptor NOD2/CARD15/IBD1 gene and protein in primary human monocytic and epithelial cells. The vitamin D receptor signals through distal enhancers in the NOD2 gene, whose function was validated by chromatin immunoprecipitation and chromatin conformation capture assays. A key downstream signaling consequence of NOD2 activation by agonist muramyl dipeptide is stimulation of NF-kappaB transcription factor function, which induces expression of the gene encoding antimicrobial peptide defensin beta2 (DEFB2/HBD2). Pretreatment with 1,25-dihydroxyvitamin D(3) synergistically induced NF-kappaB function and expression of genes encoding DEFB2/HBD2 and antimicrobial peptide cathelicidin in the presence of muramyl dipeptide. Importantly, this synergistic response was also seen in macrophages from a donor wild type for NOD2 but was absent in macrophages from patients with Crohn disease homozygous for non-functional NOD2 variants. These studies provide strong molecular links between vitamin D deficiency and the genetics of Crohn disease, a chronic incurable inflammatory bowel condition, as Crohn's pathogenesis is associated with attenuated NOD2 or DEFB2/HBD2 function.

  17. GEN 300 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 300 Ethics in an Academic Environment Assignment POWERPOINT ONLY GEN 300 Team Dynamics Instructions GEN 300 Effects of Technology Essay GEN 300 Research,Summary, and Paraphrase Activity GEN 300 Ethics in an Academic Environment Assignment PAPER ONLY GEN 300 Final Paper on Team Dynamics GEN 300 Student Web Scavenger Hunt GEN 300 Week 1 DQs GEN 300 Week 2 DQs GEN 300 Week 3 DQs GEN 300 Week 4 DQs GEN 300 Week 5 DQ

  18. GEN 105 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 105 Assignment: Reading and Retention GEN 105 Assignment: Elevator Speech GEN 105 CheckPoint: Technological Tools GEN 105 CheckPoint: Distance Learning I GEN 105 CheckPoint: Distance Learning II GEN 105 CheckPoint: Communicating in Forums GEN 105 Week 2 Discussion Questions GEN 105 Week 4 Discussion Questions GEN 105 Week 6 Discussion Questions GEN 105 Week 8 Discussion Questions GEN 105 Assignment: University Library Article Search GEN 105 Chec...

  19. Eficacia del kinesiotape como terapia complementaria en niños que presentan espasticidad.

    OpenAIRE

    Ruiz Ruiz, Ana

    2014-01-01

    ¿Se obtiene una mejora de resultados añadiendo Kinesiotape a sujetos que presentan PCI espástica comparado con un tratamiento convencional de fisioterapia? Objetivos: Evaluar la efectividad del Kinesiotape en cuanto a disminución de espasticidad y tono muscular, mejora de calidad de vida y aumento del rango articular. Material y métodos: se buscará una muestra de 238 sujetos con PCI espástica entre 5 y 20 años y que presenten una sinergia flexora en extremidad superior. Como criterios de excl...

  20. GEN 480 UOP Course Tutorial / gen480dotcom

    OpenAIRE

    2015-01-01

    GEN 480 Entire Course For more course tutorials visit www.gen480.com   GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEN 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment P...

  1. GEN 200 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 200 Week 1 Assignment- Map Out an Important Goal GEN 200 Week 1 DQ 1 GEN 200 Week 1 DQ 2 GEN 200 Week 2 Assignment- Communication and Collaboration Strategy Paper GEN 200 Week 2 DQ 1 GEN 200 Week 2 DQ 2 GEN 200 Week 3 Assignment- Student Web Scavenger Hunt GEN 200 Week 3 DQ 1 GEN 200 Week 3 DQ 2 GEN 200 Week 4 Assignment-Research Strategy Paper GEN 200 Week 4 DQ 1 GEN 200 Week 4 DQ 2 GEN 200 Week 5 DQ 1 GEN 200 Week 5 DQ 2 GEN 200 We...

  2. Poliamidas y poliesteramidas que presentan potencial aplicación en biomedicina

    Directory of Open Access Journals (Sweden)

    Marisol L. Pérez Ferrás

    2010-01-01

    Full Text Available El interés creciente del sector biomédico por la utilización de polímeros degradables y biocompatibles debido al uso que pueden tener en implantes, prótesis, suturas reabsorbibles, películas para piel artificial, injertos vasculares o en la dosificación de fármacos, ha motivado la realización de innumerables investigaciones en esta área de conocimientos dirigidas al desarrollo de nuevos materiales obtenidos a partir de productos naturales. En el trabajo se muestra el resultado de una exhaustiva revisión bibliográfica sobre los procesos de síntesis, caracterización, degradación y propiedades de poliamidas y poliesteramidas que presentan potencial aplicación en biomedicina, con el objetivo de incluir estos contenidos en los Programas de estudio de las carreras de Ingeniería y Medicina. Se enmarca en trabajos conjuntos de investigación en didáctica aplicada a nuevos materiales, que se realizan entre el Dpto. Metodológico de la Universidad de Ciencias Médicas de Holguín y el Grupo de Investigación de Materiales y ambiente de la Universidad de Holguín.

  3. 家族性克罗恩病肠黏膜通透性增加的遗传基础:CARD15 3020insC突变的作用

    Institute of Scientific and Technical Information of China (English)

    Buhner; S.; Buning; C.; Genschel; J.; 程妍(译); 陈云茹(校)

    2006-01-01

    背景与目的:遗传性肠黏膜屏障功能损伤被认为是克罗恩病(CD)的易患因素。最近,半胱氨酸蛋白酶募集域家族15号基因(CARD15)突变体被识别,并认为它与CD有关。作者推测CARD15突变可能与肠黏膜受损有关。方法:分别选取了128例非活动性CD患者,129例一级亲属(CD—R),66例非血缘性家族成员(CD—NR)和96例健康对照人群。分析三个最常见的CARD15多态现象(R702W,G908R,3020insC),并用乳果糖/甘露醇比例确定肠黏膜通透性。结果:与CD—NR和对照者相比,CD和CD—R组肠黏膜的通透性明显增加。44%CD、26%CD—R和6%CD—NR人群的乳果糖/甘露醇比例在正常范围以上,而对照组则全部在正常范围之内。CD患者的家属虽与其有相同的生活环境,但肠黏膜通透性并未增加。

  4. Estudio del efecto fotocrómico que presentan los vidriados opacificados por esfena

    Directory of Open Access Journals (Sweden)

    Gimeno, R.

    2012-04-01

    Full Text Available White glazes opacified by sphene, based on the system SiO2-CaO-TiO2, are an alternative to the glazes in which the opacifying phase is zircon. However, it has been observed that this type of glaze displays photochromism (reversible change of colour after exposure to a light source, involving the appearance of a yellowish shade after the glaze has been subjected to sunlight for several hours, which then disappears after a few days. This effect has led to limited use of this type of glaze. In this study, a method of quantifying the photochromic effect by means of a UV light source is established. The microstructural characterisation of glazes with a pronounced tendency to display a photochromic effect has allowed the possible causes of the photochromic effect to be determined. Finally, a series of tests have been conducted that show that the photochromic effect is related to the impurities present in the glaze, as well as to the type and quantity of crystalline phases contained in the glaze.

    Los vidriados blancos opacificados por esfena, basados en el sistema SiO2-CaO-TiO2, son una alternativa a los vidriados en los que la fase opacificante es el circón. Sin embargo, se ha observado que este tipo de vidriados presentan fotocromismo (cambio de color reversible tras la exposición a una fuente de luz, el cual consiste en la aparición de una tonalidad amarillenta tras someter el vidriado a la luz solar durante varias horas, que desaparece al cabo de varios días. Este efecto hace que la utilización de este tipo de vidriados no se haya generalizado. En el presente trabajo se establece un método para la cuantificación del efecto fotocrómico mediante el uso de una fuente de luz ultravioleta. La caracterización microestructural de vidriados con distinta tendencia a presentar el efecto fotocrómico ha permitido establecer las posibles causas que provocan el efecto fotocrómico. Finalmente, se

  5. GenBank

    Data.gov (United States)

    U.S. Department of Health & Human Services — GenBank is the NIH genetic sequence database, an annotated collection of all publicly available DNA sequences. GenBank is designed to provide and encourage access...

  6. GEN 499 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 499 Week 1 DQ 1 Final Research Paper Topic and Plan GEN 499 Week 1 DQ 2 Social Media GEN 499 Week 2 DQ 1 Professional Resume and Cover Letter GEN 499 Week 2 Assignment Critiquing Internet Sources GEN 499 Week 3 DQ 1 Social Capital GEN 499 Week 3 DQ 2 Federal Policy GEN 499 Week 3 Assignment Annotated Bibliography GEN 499 Week 4 DQ 1 Call to Action GEN 499 Week 4 DQ 2 Final Research Paper Progress GEN 499 Week 4 Critical Thinking Quiz GEN 499 Week 5 ...

  7. GEN 480 Courses/sanptutorial

    OpenAIRE

    2015-01-01

    GEN 480 Week 1 Individual Assignment Ethics Awareness Inventory GEN 480 Week 1 DQ 1 GEN 480 Week 1 DQ 2 GEN 480 Week 1 DQ 3 GEN 480 Week 1 DQ 4 GEM 480 Week 1 Summary GEN 480 Week 2 Individual Assignment Ethics Awareness Inventory Analysis GEN 480 Week 2 Individual Assignment Professional Workplace Dilemma Paper GEN 480 Week 2 Learning Team Assignment Skills Assessment Paper and Matrix GEN 480 Week 2 DQ 1 GEN 480 Week 2 DQ 2 GEN 480 Week 2 DQ 3 ...

  8. GEN 480 UOP TUTORIAL / Uoptutorial

    OpenAIRE

    2015-01-01

    For more course tutorials visit www.uoptutorial.com           GEN 480 Week 1 DQ 1  GEN 480 Week 1 DQ 2  GEN 480 Week 1 DQ 3  GEN 480 Week 1 DQ 4  GEN 480 Week 1 Individual AssignmentEthics Awareness  GEN 480 Week 1 Summary  GEN 480 Week 2 DQ 1  GEN 480 Week 2 DQ 2  GEN 480 Week 2 DQ 3  GEN 480 Week 2 DQ 4  GEN 480 Week 2 Individual Assignment Ethics Awareness &...

  9. Grado de conciencia que presentan los profesores del aspecto comunicativo no-verbal durante el acto didáctico

    Directory of Open Access Journals (Sweden)

    Isabel CUADRADO GORDILLO

    2009-11-01

    Full Text Available El trabajo que se presenta forma parte de una investigación más amplia titulada «Comunicación no-verbal e implicaciones didácticas en el aula: Desarrollo y análisis de casos de conciencia propia», cuyo objetivo principal consiste en describir, analizar e interpretar el grado de conciencia que los profesores presentan de sus comportamientos no-verbales durante el proceso interactivo didáctico.

  10. Capítulo II: consideraciones generales sobre el mejoramiento genético de tomate, Lycopersicon esculentum, Mill

    OpenAIRE

    Vallejo Cabrera Franco Alirio

    1994-01-01

    Objetivos del programa de mejoramiento genético del tomate: Los objetivos del programa de mejoramiento dependen de los problemas que se presentan en el cultivo en las diferentes zonas de Colombia, del uso que se le va a dar al fruto (mesa o industria) y especialmente de las condiciones y recursos del agricultor, procesador y consumidor.

  11. rs1004819 is the main disease-associated IL23R variant in German Crohn's disease patients: combined analysis of IL23R, CARD15, and OCTN1/2 variants.

    Directory of Open Access Journals (Sweden)

    Jürgen Glas

    Full Text Available BACKGROUND: The IL23R gene has been identified as a susceptibility gene for inflammatory bowel disease (IBD in the North American population. The aim of our study was to test this association in a large German IBD cohort and to elucidate potential interactions with other IBD genes as well as phenotypic consequences of IL23R variants. METHODS: Genomic DNA from 2670 Caucasian individuals including 833 patients with Crohn's disease (CD, 456 patients with ulcerative colitis (UC, and 1381 healthy unrelated controls was analyzed for 10 IL23R SNPs. Genotyping included the NOD2 variants p.Arg702Trp, p.Gly908Arg, and p.Leu1007fsX1008 and polymorphisms in SLC22A4/OCTN1 (1672 C-->T and SLC22A5/OCTN2 (-207 G-->C. RESULTS: All IL23R gene variants analyzed displayed highly significant associations with CD. The strongest association was found for the SNP rs1004819 [P = 1.92x10(-11; OR 1.56; 95 % CI (1.37-1.78]. 93.2% of the rs1004819 TT homozygous carriers as compared to 78% of CC wildtype carriers had ileal involvement [P = 0.004; OR 4.24; CI (1.46-12.34]. The coding SNP rs11209026 (p.Arg381Gln was protective for CD [P = 8.04x10(-8; OR 0.43; CI (0.31-0.59]. Similar, but weaker associations were found in UC. There was no evidence for epistasis between the IL23R gene and the CD susceptibility genes CARD15 and SLC22A4/5. CONCLUSION: IL23R is an IBD susceptibility gene, but has no epistatic interaction with CARD15 and SLC22A4/5. rs1004819 is the major IL23R variant associated with CD in the German population, while the p.Arg381Gln IL23R variant is a protective marker for CD and UC.

  12. Genética de la diabetes mellitus tipo 2

    OpenAIRE

    Carmen Carrillo; Arturo Panduro Cerda

    2001-01-01

    Factores genéticos predisponen a la diabetesmellitus (DM) tipo 2 y el desarrollo de la enfermedad depende en gran parte de la alimentación y actividad física (factores ambientales).Existen familias cuyos miembros presentan DMtipo 2 solamente o bien diferentes tipos de diabetes. En general se presenta un patrón de herencia multifactorial, rara vez autosómico dominante o mitocondrial. El riesgo que tienen losfamiliares de pacientes con DM tipo 2 se establece con el valor lambda el cual depende ...

  13. GenBank

    Science.gov (United States)

    Benson, Dennis A.; Cavanaugh, Mark; Clark, Karen; Karsch-Mizrachi, Ilene; Lipman, David J.; Ostell, James; Sayers, Eric W.

    2017-01-01

    GenBank® (www.ncbi.nlm.nih.gov/genbank/) is a comprehensive database that contains publicly available nucleotide sequences for 370 000 formally described species. These sequences are obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole genome shotgun (WGS) and environmental sampling projects. Most submissions are made using the web-based BankIt or the NCBI Submission Portal. GenBank staff assign accession numbers upon data receipt. Daily data exchange with the European Nucleotide Archive (ENA) and the DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through the NCBI Nucleotide database, which links to related information such as taxonomy, genomes, protein sequences and structures, and biomedical journal literature in PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. Recent updates include changes to policies regarding sequence identifiers, an improved 16S submission wizard, targeted loci studies, the ability to submit methylation and BioNano mapping files, and a database of anti-microbial resistance genes. PMID:27899564

  14. DETEKSI GEN-GEN PENYANDI FAKTOR VIRULENSI PADA BAKTERI VIBRIO

    Directory of Open Access Journals (Sweden)

    Ince Ayu Khairani Kadriah

    2011-04-01

    menggunakan isolat bakteri yang diisolasi dari budidaya udang windu di berbagai daerah di Sulawesi Selatan dan Jawa. Pada penelitian ini digunakan primer spesifik untuk mendeteksi gen-gen virulen toxR gene, hemolysin (vvh gene, dan GyrB gene dengan metode PCR. Dari 35 isolat yang diisolasi, 20 isolat terdeteksi memiliki gen virulensi dan 8 di antaranya memiliki dua gen virulen. Spesies bakteri yang memiliki gen virulen adalah: V.harveyi, V. parahaemolyticus, V. mimicus, dan V. campbelli

  15. Respuesta a: comentarios sobre el articulo "Prevalencia de insomnio y deterioro de la calidad de vida en posmenopausicas que presentan oleadas de calor residentes en el Caribe Colombiano"

    National Research Council Canada - National Science Library

    Monterrosa-Castro, Alvaro

    2012-01-01

    .... El articulo "Prevalencia de insomnio y deterioro de la calidad de vida en posmenopausicas que presentan oleadas de calor residentes en el Caribe colombiano" (1) hace parte del proyecto de Investigacion Morfem que intenta explorar calidad y disturbios del sueno en mujeres climatericas colombianas (4059 anos de edad), el cual es una rama del pro...

  16. GenBank

    OpenAIRE

    Burks, Christian; Cassidy, Maxxwell; Cinkosky, Michael J.; Cumella, Karen E.; Gilna, Paul; Hayden, Jamie E.-D.; Keen, Gifford M.; Kelley, Tom A.; Kelly, Michael; Kristofferson, David; Ryals, Julie

    1991-01-01

    The GenBank nucleotide sequence database now contains sequence data and associated annotation corresponding to 56,000,000 nucleotides in 45,000 entries. The input stream of data coming into the database has largely been shifted to direct submissions from the scientific community on electronic media. The data have been installed in a relational database management system and are made available in this form through on-line access, and through various network and off-line computer-readable media...

  17. XIAO Pei-gen

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Academician of Chinese Academy of Engineering Editor-in-chief of Chinese Herbal Medicines(CHM)Honorary director of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing100193,China Tel/Fax:+86-10-62894462 E-mail:xiaopg@public.bta.net.cn Professor XIAO Pei-gen is the founder of the Institute of Medicinal Plant Development(IMPLAD),Chinese Academy of Medical Sciences(CAMS).He is also one of the founders and leading

  18. Selección natural, genética cuantitativa y evolución en culebras

    OpenAIRE

    Javier Manjarrez Silva

    2001-01-01

    Se describen los conceptos, términos y técnicas empleadas en la genética cuantitativa y la selección natural, en particular de los componentes de la varianza fenotípica y sus técnicas para reducirla. Se utiliza a las serpientes como ejemplo por las características que presentan y se justifica la aplicación de este tipo de estudios, en particular para las estimaciones de la heredabilidad de caracteres cuantitativos de las culebras, con el fin de visualizar su posible base genética e implicacio...

  19. Selección natural, genética cuantitativa y evolución en culebras

    Directory of Open Access Journals (Sweden)

    Javier Manjarrez Silva

    2001-01-01

    Full Text Available Se describen los conceptos, términos y técnicas empleadas en la genética cuantitativa y la selección natural, en particular de los componentes de la varianza fenotípica y sus técnicas para reducirla. Se utiliza a las serpientes como ejemplo por las características que presentan y se justifica la aplicación de este tipo de estudios, en particular para las estimaciones de la heredabilidad de caracteres cuantitativos de las culebras, con el fin de visualizar su posible base genética e implicaciones evolutivas.

  20. Properties of Sin, Gen, and SinGen clusters

    Science.gov (United States)

    Dong, Yi; ur Rehman, Habib; Springborg, Michael

    2015-01-01

    The structures of Sin, Gen, and SinGen clusters with up to 44 atoms have been determined theoretically using an unbiased structure-optimization method in combination with a parametrized, density-functional description of the total energy for a given structure. By analyzing the total energy in detail, particularly stable clusters are identified. Moreover, general trends in the structures are identified with the help of specifically constructed descriptors.

  1. Identificación en lazo cerrado mediante algoritmos genéticos

    OpenAIRE

    Alfredo Gómez Infante; Abelardo del Pozo Quintero; Alberto Aguado Behar

    2010-01-01

    La identificación  en lazo cerrado de sistemas continuos es un problema de optimización no lineal que resulta de difícil solución mediante métodos convencionales.  En este trabajo se propone el uso de algoritmos genéticos (AG) para esta tarea y se muestra mediante simulaciones que los modelos obtenidos pueden reproducir fielmente el comportamiento de la planta, aun en el caso de sistemas inestables o que presentan algún tipo de no-linealidad. Estos modelos pueden utilizarse para el ajuste ...

  2. Programa de ejercicio físico para ayudar a la atención de algunas conductas que presentan niñas abusadas sexualmente e institucionalizadas

    OpenAIRE

    2004-01-01

    Tesis texto completo Analiza si una intervención de ejercicio físico programado, puede ayudar en la atención de algunas conductas que presentan niñas abusadas sexualmente e institucionalizadas. Realiza un diagnóstico de necesidades y del perfil de entrada de cada una de las niñas que forman parte del grupo, con el fin de diseñar un programa acorde a sus necesidades. Diseña y ejecuta un programa de ejercicio físico adaptado a las necesidades del grupo de niñas y considera las posibilidades ...

  3. Tratamientos ortopodológicos en pacientes que presentan transtornos del equilibrio estático y dinámico.

    OpenAIRE

    1989-01-01

    Centrándonos en la exploración del paciente que ha sufrido o sufre caídas frecuentes, presentamos unas pruebas específicas para valorar el alcance de las alteraciones que provocan pérdidas de equilibrio. Exposición de varios casos clínicos que presentan afecciones podológicas que alteran la estabilidad, y ofreceremos una alternativa de Tratamiento Ortopodológico demostrando su acción en favor del mantenimiento del equilibrio. Conclusiones referidas al diagnóstico y expectativas de Tratamiento...

  4. 宝腾GEN-2

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    宝腾 GEN-2是由马来西亚宝腾汽车公司和莲花工程公司(Lotus Engineering)耗时4年共同开发的,也是宝腾收购莲花后设计的第一款新车。车名GEN-2也就是 GENRERATION 2的缩写。在 GEN-2的开发过程中,造型设计和工程设计是由宝腾公司完成的,莲花工程公司对 GEN-2进行了底盘调校,莲花设计中心(Lotus DesignStudio)完成了内饰设计。发动机则是由宝腾汽车公司开发、莲花工程公司调校的名为 Campro(Campro 是

  5. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia. Bases genéticas de la esquizofrenia: "Nurture vrs Nature

    Directory of Open Access Journals (Sweden)

    Henriette Raventós-Vorst

    2003-01-01

    Full Text Available El presente artículo revisa la evidencia científica que muestra la heredabilidad de la esquizofrenia, su forma de herencia compleja y la posible heterogeneidad genética y ambiental. Se presentan las regiones cromosómicas que han sido ligadas a la enfermedad y algunos de los genes candidatos. El objetivo es presentar los resultados más importantes en el campo de la investigación genética de la enfermedad. Aunque se acepta que factores ambientales deben estar presentes en la etiopatogenia de la enfermedad, no se profundiza en ellos. Finalmente, se comenta el modelo lamarquiano sugerido por el Prof.. Bolaños. El fin es transmitir que en la actualidad no hay contradicción entre el modelo biologista o psicológico que explicaban esta enfermedad. La concepción moderna une ambos modelos: se considera una enfermedad del neurodesarrollo en la que participan factores genéticos, factores epigenéticos y noxas ambientales, incluyendo los factores psicosociales.

  6. FutureGen Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Cabe, Jim; Elliott, Mike

    2010-09-30

    This report summarizes the comprehensive siting, permitting, engineering, design, and costing activities completed by the FutureGen Industrial Alliance, the Department of Energy, and associated supporting subcontractors to develop a first of a kind near zero emissions integrated gasification combined cycle power plant and carbon capture and storage project (IGCC-CCS). With the goal to design, build, and reliably operate the first IGCC-CCS facility, FutureGen would have been the lowest emitting pulverized coal power plant in the world, while providing a timely and relevant basis for coal combustion power plants deploying carbon capture in the future. The content of this report summarizes key findings and results of applicable project evaluations; modeling, design, and engineering assessments; cost estimate reports; and schedule and risk mitigation from initiation of the FutureGen project through final flow sheet analyses including capital and operating reports completed under DOE award DE-FE0000587. This project report necessarily builds upon previously completed siting, design, and development work executed under DOE award DE-FC26- 06NT4207 which included the siting process; environmental permitting, compliance, and mitigation under the National Environmental Policy Act; and development of conceptual and design basis documentation for the FutureGen plant. For completeness, the report includes as attachments the siting and design basis documents, as well as the source documentation for the following: • Site evaluation and selection process and environmental characterization • Underground Injection Control (UIC) Permit Application including well design and subsurface modeling • FutureGen IGCC-CCS Design Basis Document • Process evaluations and technology selection via Illinois Clean Coal Review Board Technical Report • Process flow diagrams and heat/material balance for slurry-fed gasifier configuration • Process flow diagrams and heat/material balance

  7. Summary of CPAS Gen II Parachute Analysis

    Science.gov (United States)

    Morris, Aaron L.; Bledsoe, Kristin J.; Fraire, Usbaldo, Jr.; Moore, James W.; Olson, Leah M.; Ray, Eric

    2011-01-01

    The Orion spacecraft is currently under development by NASA and Lockheed Martin. Like Apollo, Orion will use a series of parachutes to slow its descent and splashdown safely. The Orion parachute system, known as the CEV Parachute Assembly System (CPAS), is being designed by NASA, the Engineering and Science Contract Group (ESCG), and Airborne Systems. The first generation (Gen I) of CPAS testing consisted of thirteen tests and was executed in the 2007-2008 timeframe. The Gen I tests provided an initial understanding of the CPAS parachutes. Knowledge gained from Gen I testing was used to plan the second generation of testing (Gen II). Gen II consisted of six tests: three singleparachute tests, designated as Main Development Tests, and three Cluster Development Tests. Gen II required a more thorough investigation into parachute performance than Gen I. Higher fidelity instrumentation, enhanced analysis methods and tools, and advanced test techniques were developed. The results of the Gen II test series are being incorporated into the CPAS design. Further testing and refinement of the design and model of parachute performance will occur during the upcoming third generation of testing (Gen III). This paper will provide an overview of the developments in CPAS analysis following the end of Gen I, including descriptions of new tools and techniques as well as overviews of the Gen II tests.

  8. GenLab, Laboratorio Virtual de Genética

    Directory of Open Access Journals (Sweden)

    García Sergio

    2000-12-01

    Full Text Available

    GenLab es el nombre que tiene el software diseñado por nosotros, en el cual se modela el proceso meiótico y la fecundación en organismos diploides. El objetivo de esta aplicación es ilustrar el resultado de un cruce determinado, tratando de ser lo más ajustados a la realidad. La modelación de la reproducción sexual se realiza internamente y el GenLab se limita a presentar los resultados según el número de descendencia seleccionado para un cruce específico, esto significa que se puede escoger una gran cantidad de características para los parentales y se puede estudiar la frecuencia de estos en la descendencia. El modelo cuenta con base de datos donde están almacenados algunos de los locus de Drosophila melanogaster junto con su ubicación en centimorgans 1. EI propósito de este modelo es servir como herramienta pedagógica  y didáctica tanto en universidades como en colegios, facilitando el aprendizaje de algunos principios básicos de la genética, por lo cual puede ser usado si se cuenta con una conexión a Internet y un navegador visitando http://biologia.unal.edu.co/fidel.

  9. TrafficGen Architecture Document

    Science.gov (United States)

    2016-01-01

    Poisson, Jitter, and Clone . Researchers can experience an added dimension of network traffic visualization by pairing TrafficGen with the NRL Scripted...MVC Classes The top-level MVC classes control the workspace of the application, specifically the menu and the way the nodes and events are rendered...This is a container class that holds singleton instances of the view classes employed by this application. Because of the way this application is

  10. Refuerzo de puentes por cambio de esquema estructural: optimización mediante algoritmo genético

    OpenAIRE

    Valenzuela, Matías A.; Casas Rius, Joan Ramon

    2011-01-01

    La presente comunicación entrega un estudio detallado del proceso constructivo y de tesado para el refuerzo de puentes con tipología longitudinal de vigas continuas convirtiéndolos en puentes en arco atirantado (tipo network). Se presentan las etapas básicas propuestas en aspectos de construcción, se establecen las hipótesis del estudio de tesado e implementa el método de tesado a partir de la optimización automatizada mediante algoritmos genéticos, definiendo criterios como: variables de ...

  11. Enfermedades infecciosas más frecuentes que se presentan en los recién nacidos prematuros en el Hospital Provincial Docente Ambato en el área de neonatología de enero a julio del 2014

    OpenAIRE

    Chiliquinga Sarabia, Norma Susana

    2015-01-01

    Lamentablemente no siempre ni en todos los escenarios de actuación de neonatología existen recursos disponibles que permitan establecer el diagnóstico etiológico de las infecciones que presentan los recién nacidos, por lo cual el personal responsable de atender a estos pacientes tiene que valerse de antecedentes o manifestaciones clínicas orientadoras en este sentido. El objetivo de este estudio fue analizar las enfermedades infecciosas más frecuentes que se presentan en los recién nacidos pr...

  12. Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.

    Science.gov (United States)

    Ezaki, T; Kawamura, Y; Li, N; Li, Z Y; Zhao, L; Shu, S

    2001-07-01

    Members of genus Peptostreptococcus have previously been found to be distantly related to the type species, Peptostreptococcus anaerobius, on the basis of 16S rDNA sequence similarities. They were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. The reclassification of the species of these three groups into three new genera, Peptoniphilus gen. nov., Anaerococcus gen. nov. and Gallicola gen. nov., is proposed. The genus Peptoniphilus gen. nov. includes the following butyrate-producing, non-saccharolytic species that use peptone and amino acids as major energy sources: Peptoniphilus asaccharolyticus comb. nov. (type species), Peptoniphilus lacrimaris comb. nov., Peptoniphilus harei comb. nov., Peptoniphilus indolicus comb. nov. and Peptoniphilus ivorii comb. nov. The genus Anaerococcus gen. nov. contains the saccharolytic, butyrate-producing species Anaerococcus prevotii comb. nov. (type species), Anaerococcus tetradius comb. nov., Anaerococcus lactolyticus comb. nov., Anaerococcus hydrogenalis comb. nov., Anaerococcus vaginalis comb. nov. and Anaerococcus octavius sp. nov. The genus Gallicola gen. nov. contains a single species, Gallicola barnesae comb. nov.

  13. Estructura genética-poblacional de gatos domésticos (Felis catus, usando marcadores fenotípicos en Santa Marta, Colombia

    Directory of Open Access Journals (Sweden)

    Luis Alfonso Causil Vargas

    2017-01-01

    Full Text Available Las variaciones en la textura y color del pelo a menudo difieren entre razas de animales, son el resultado del polimorfismo de un grupo de genes, expresados en marcadores fenotípicos, los cuales; se han convertido en una herramienta útil para el estudio de la genética de poblaciones, puesto que presentan diferentes tipos de herencia y acciones génicas. El objetivo de nuestro trabajo fue analizar la diversidad y estructura genética de las poblaciones de gatos domésticos (Felis catus en Santa Marta, Colombia; mediante genes del pelaje. Metodológicamente se realizó un muestreo de gatos en seis barrios de las ciudades de Santa Marta, por descripciones morfológicas se determinaron los fenotipos del pelaje y con base a ello se evaluaron los índices de variabilidad genética: frecuencias alélicas, equilibrio de Hardy Weinberg, diversidad genética, estructura poblacional, flujo génico y distancias genéticas. Resultados: Se obtuvo una elevada frecuencia de los marcadores O y S; los marcadores evidenciaron ausencia de equilibrio de Hardy – Weinberg. Las poblaciones mostraron un nivel moderado de diferenciación (GST= 2 0,110. Comparada con poblaciones colombianas Riohacha y Santa Marta son cercanas genéticamente y comparten similitudes con las poblaciones de la costa Caribe. En conclusión, Santa Marta presenta pocas diferencias en la estructura genética de gatos domésticos, y altos niveles de selección en ciertos fenotipos.

  14. Teleport Generation 3 (Teleport Gen 3)

    Science.gov (United States)

    2016-03-01

    8596 DSN Fax: Date Assigned: September 4, 2014 Program Information Program Name Teleport Generation 3 (Teleport Gen 3) DoD Component DoD The...2015 Approved APB Component Acquisition Executive (CAE) Approved Acquisition Program Baseline (APB) dated June 15, 2015 Teleport Gen 3 2016 MAR...System Network (DISN). The DoD Teleport upgrades selected sites from the Standardized Tactical Entry Point (STEP) program, which only provides reach

  15. Diversidad genética de Azospirillum brasilense en suelos cultivados con maíz con labranza convencional y de conservación

    OpenAIRE

    D. Espinosa-Victoria; L. Hernández-Flores; L. López-Reyes

    2006-01-01

    El objetivo del presente estudio fue determinar la diversidad genética de Azospirillum brasilense en suelos cultivados con maíz con labranza convencional y de conservación durante las épocas de primavera e invierno. El estudio se realizó en parcelas de dos campos experimentales en Santa Isabel de Ajuno, Michoacán y Pabellón de Arteaga, Aguascalientes, así como en el campo experimental en Villadiego, Guanajuato; que presentan un Andosol, un Calcisol háplico y un Vertisol pélico, respectivament...

  16. Trabajar genética y enfermedades en secundaria integrando la modelización y la argumentación científica

    Directory of Open Access Journals (Sweden)

    Noa Ageitos

    2017-01-01

    Full Text Available Se presenta el diseño de una unidad didáctica para secundaria que tiene como objetivo principal el aprendizaje de genética y enfermedades humanas en interacción con el desempeño de prácticas científicas de argumentación y modelización. La secuencia aborda diversas enfermedades con componente genético que requieren la transferencia del modelo de expresión de los genes para su adecuada comprensión. La secuencia pretende servir como recurso al profesorado de ciencias interesado en introducir las prácticas científicas y el pensamiento crítico para promover la toma de decisiones. Se presentan algunos resultados relevantes de la puesta en práctica de la secuencia para su implementación efectiva por el profesorado.

  17. Debroyerella gen. nov. and Ulladulla gen. nov., two new lysianassoid genera (Crustacea, Amphipoda, Lysianassoidea).

    Science.gov (United States)

    Lowry, J K; Kilgallen, N M

    2015-02-19

    Two new genera and a new species of lysianassoid amphipods are described. Debroyerella gen. nov. is described for three Antarctic species previously assigned to the genus Cheirimedon. Ulladulla gen. nov. is described to accommodate the new species U. selje, from Australian waters. Diagnostic descriptions are given for the genera and all species are described in full.

  18. Unleashing Gen Y: Marketing Mars to Millennials

    Science.gov (United States)

    Leahy, Bart D.; Hidalgo, Loretta; Kloberdanz, Cassie

    2007-01-01

    Space advocates need to engage Generation Y (born 1977-1999).This outreach is necessary to recruit the next generation of scientists and engineers to explore Mars. Space advocates in the non-profit, private, and government sectors need to use a combination of technical communication, marketing, and politics, to develop messages that resonate with Gen Y. Until now, space messages have been generated by and for college-educated white males; Gen Y is much more diverse, including as much as one third minorities. Young women, too, need to be reached. My research has shown that messages emphasizing technology, fun, humor, and opportunity are the best means of reaching the Gen Y audience of 60 million (US population is 300 million). The important things space advocates must avoid are talking down to this generation, making false promises, or expecting them to "wait their turn" before they can participate. This is the MTV generation! We need to find ways of engaging Gen Y now to build a future where human beings can live and work on the planet Mars. In addition to the messages themselves, advocates need to keep up with Gen Y' s social networking and use of iPods, cell phones, and the Internet. NASA and space advocacy groups can use these tools for "viral marketing," where young people share targeted space-related information via cell phones or the Internet because they like it. Overall, Gen Y is a socially dynamic and media-savvy group; advocates' space messages need to be sincere, creative, and placed in locations where Gen Y lives. Mars messages must be memorable!

  19. Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov.

    Science.gov (United States)

    Kurtzman, Cletus P; Robnett, Christie J

    2014-05-01

    Three new non-ascosporic, ascomycetous yeast genera are proposed based on their isolation from currently described species and genera. Phylogenetic placement of the genera was determined from analysis of nuclear gene sequences for D1/D2 large subunit rRNA, small subunit rRNA, translation elongation factor-1α and RNA polymerase II, subunits B1 and B2. The new taxa are: Deakozyma gen. nov., type species Deakozyma indianensis sp. nov. (type strain NRRL YB-1937, CBS 12903); Danielozyma gen. nov., type species Danielozyma ontarioensis comb. nov. (type strain NRRL YB-1246, CBS 8502); D. litseae comb. nov. (type strain NRRL YB-3246, CBS 8799); Middelhovenomyces gen. nov., type species Middelhovenomyces tepae comb. nov. (type strain NRRL Y-17670, CBS 5115) and M. petrohuensis comb. nov. (type strain NRRL Y-17663, CBS 8173).

  20. Análisis genético de la longevidad en conejas de producción cárnica. Constitución y evaluación de una línea Longevo - Productiva de conejos

    OpenAIRE

    Sánchez Serrano, Juan Pablo

    2008-01-01

    En primer lugar se revisan las distintas definiciones que para la longevidad se han propuesto y se describen brevemente los métodos de análisis empleados para estimar parámetros genéticos y predecir valores de cría dentro de cada una de estas definiciones. A continuación se presentan en detalle los métodos de análisis de supervivencia, que son los empleados en este trabajo. Finalmente se hace una revisión bibliográfica de las estimas de parámetros genéticos que para longevidad se han obtenido...

  1. ISOLASI DAN ANALISIS GEN HORMON PERTUMBUHAN LELE (Clarias gariepinus Burch.

    Directory of Open Access Journals (Sweden)

    Ibnu Dwi Buwono

    2012-12-01

    CAGGGTGCAGTTGGAATCC-3’ dapat mengkopi sekuen gen GH lele dengan ukuran fragmen PCR sekitar 1.400 bp. Sementara amplikon gen GH American catfish (Rhamdia quelen menggunakan primer Amc-GH-F dan Amc-GH-R sebesar 1.465 bp. Hasil analisis sekuensing gen penyandi GH menggunakan program BlastP dan Genetyx versi 7.0, menunjukkan bahwa sekuen gen penyandi GH lele dumbo memiliki homologi 80% dengan sekuen GH C. gariepinus pada bank gen (no. aksesi AF 416488.1, sehingga sebagian besar sekuen gen penyandi hormon pertumbuhan ikan tersebut dapat diamplifikasi secara in vitro.

  2. Genética y derechos humanos

    OpenAIRE

    Gómez-Ojero y Martínez, Luis

    2016-01-01

    [ES]La tesis se propone realizar un análisis de los temás más novedosos en ciencia genética y del estado de la cuestión relativa a los Derechos Humanos y la Genética partiendo de los textos más relevantes, tanto a nivel jurídico como meramente institucional que, como en el caso de Naciones Unidas, tanta importancia tienen en la influencia que de facto suele lograr sobre el Derecho Internacional, el Derecho de la Unión Europea y, por reflejo, indirectamente, en los Ordenamientos Jurídicos Inte...

  3. Zum gotischen gen. pl. auf-ë

    Directory of Open Access Journals (Sweden)

    M. Grošelj

    1964-12-01

    Full Text Available Bekanntlich ist die got. Endung -ē eine Besonderheit, der in den übrigen germ. Sprachen nichts Vergleichbares gegenübersteht. Man nimmt wohl -allgemein an, dass es sich um eine innergot. Neuschöpfung handelt; auch ist eine idg. Endung des Gen. Pl. -ēm; linbelegt. Folgender Erklärungsversuch geht von der Annahme aus, dass die Triebkraft für dfese Neuschöpfupg in der Neigung zur Differenzierung des Maskulinums vom Femininum auf -ō (gibō : dagē zu suchen ist. Diese Neigung ist besonders im Nom. und Gen. bemerkbar.

  4. Dificultades que presentan los estudiantes para profesor de matemáticas en la comprensión del lenguaje matemático utilizado en las demostraciones geométricas euclidianas

    OpenAIRE

    Córdoba, Paola; Quintana, Yadid

    2012-01-01

    El lenguaje que es usado en las demostraciones geométricas presentadas por Euclides en su texto Los elementos; particularmente las traducciones entre códigos en dicho lenguaje, serán la base de este artículo. Se expone un análisis referente a las dificultades que presentan los estudiantes para profesor de matemáticas en la comprensión del lenguaje matemático utilizado en las demostraciones geométricas de Euclides. También es descrita la demostración geométrica y una clasificación establecida ...

  5. Efecto del consumo de chía (Salvia hispánica) sobre los síntomas de estreñimiento que presentan los estudiantes de una universidad particular de Lima Este, 2014

    OpenAIRE

    Bernal Altamirano, Eidi; E.P. de Nutrición Humana - Lima; Iñaguazo Trávez, Johanna Jessenia; Chanducas Lozano, Bertha

    2016-01-01

    Objetivo: Este trabajo pretende determinar el efecto del consumo de chía (Salvia hispánica) sobre los síntomasde estreñimiento que presentan los estudiantes de una universidad privada de Lima Este. Material y Métodos: Elestudio es de diseño pre-experimental y de corte longitudinal. Los participantes fueron 20 estudiantes entre 18 a25 años con síntomas de estreñimiento. Para el tratamiento fueron divididos en dos subgrupos, donde el subgrupoA consumió 25 gr. y el subgrupo B 15 gr. de semillas ...

  6. Programa para mejorar las relaciones interpersonales en el aula de 5-6 años con niños que viven en familias de acogida y presentan problemas de conducta.

    OpenAIRE

    Camba-Silveti, Maitane

    2014-01-01

    En el aula de Educación Infantil es muy importante establecer y mantener un ambiente de relaciones interpersonales que desarrolle los aspectos intelectuales, sociales, morales, emocionales y de personalidad. El objetivo fundamental de este estudio ha sido el desarrollo de una serie de actividades que contribuyan a que los alumnos de 5-6 años que se encuentran en régimen de acogimiento familiar y que presentan problemas de conducta mejoren sus relaciones interpersonales dentro del aula de Educ...

  7. Representaciones sociales sobre la escuela que presentan un grupo de niñas internas de una institución educativa de la zona centro del departamento del Huila

    OpenAIRE

    Ordoñez Andrade, Gina Marcela

    2011-01-01

    Tesis (Maestría en Educación y Desarrollo Humano). Universidad de Manizales. Facultad de Ciencias Sociales y Humanas. Cinde, 2011 El estudio devela las representaciones sociales sobre la escuela que construyen 4 niñas internas de una institución educativa de la zona centro del departamento del Huila; a partir de entrevistas en profundidad se identificaron las creencias, sentimientos, imágenes y prácticas sociales que presentan las niñas frente a la escuela...

  8. La educación psicomotriz en su contribución al desarrollo del lenguaje en niños que presentan necesidades específicas de apoyo educativo

    OpenAIRE

    Rodríguez, María Teresa; Gómez, Isabel María; Prieto Ayuso, Alejandro; Pedro, Gil Madrona

    2017-01-01

    La presente investigación pretende mostrar la influencia y contribución de la intervención psicomotriz en el desarrollo del lenguaje en aquellos niños con necesidades específicas de apoyo educativo . Se lleva a cabo un programa intervención psicomotriz basado en el movimiento y el juego motor, como complemento a la intervención en logopedia durante tres meses de duración con tres niños de entre 4 y 5 años que presentan determinadas dificultades en el desarrollo del lenguaje como p...

  9. Colon Cancer on The Rise Among Gen Xers, Millennials

    Science.gov (United States)

    ... Colon Cancer on the Rise Among Gen Xers, Millennials And an old adversary -- the obesity epidemic -- may ... their early 50s and younger -- Gen Xers and millennials -- are experiencing significant increases in colon and rectal ...

  10. Diversidad genética de Dioscorea trifida “sachapapa” de cinco cuencas hidrográficas de la amazonía peruana

    Directory of Open Access Journals (Sweden)

    Jhonatan Pérez Arévalo

    2013-12-01

    Full Text Available Dioscorea trifida “sachapapa” es una de las especies promisorias amazónicas que podemos considerarla huérfana de la ciencia, por las escasas investigaciones que hay sobre esta especie. El objetivo de esta investigación fue determinar la diversidad genética intra e interpoblacional de D. trifida de cinco cuencas hidrográficas de la amazonía peruana. Las hojas fueron colectadas de una colección de germoplasma y purificó el ADN con métodos estándares. El polimorfismo genético se evaluó con la técnica RAPD y los parámetros de genética poblacional fueron estimados con el programa POPGENE. Los análisis espectrofotométrico (A260/A280=1,7±0,1 y electroforético (bandas de ADN íntegras mostraron que el ADN purificado fue de alta calidad. Asimismo, la cantidad obtenida fue apropiada para estudios de diversidad genética (rendimiento promedio = 582±248 mg ADN/mg hojas. La diversidad genética intrapoblacional más alta se encontró en la cuenca del Itaya (h = 0,24±0,11 y la más baja en la cuenca del Marañón (h = 0,10±0,04. Adicionalmente, la diversidad genética interpoblacional más alta se registró entre las poblaciones de Itaya vs Ucayali (GST = 1,00, mientras que la más baja entre las poblaciones de Nanay vs Tapiche (GST = 0,07. En conclusión, D. trifida muestra variación en su diversidad genética intra e interpoblacional en las cinco cuencas hidrográficas de la amazonía peruana, siendo la cuenca del Itaya la que presenta mayor diversidad genética intrapoblacional y las poblaciones de Itaya vs Ucayali las que presentan mayor diversidad genética interpoblacional, que en parte se atribuyen al flujo de genes diferencial entre las poblaciones analizadas.

  11. Papel de las mutaciones en el gen de la presenilina 1 en la patogenia de la enfermedad de Alzheimer

    Directory of Open Access Journals (Sweden)

    Francisco Lopera

    2003-01-01

    Full Text Available La Enfermedad de Alzheimer (EA es un trastorno neurológico con devastadores efectos físicos, económicos, familiares y sociales. En la actualidad es considerada como un problema de salud pública y las perspectivas para los próximos años son poco alentadoras, teniendo en cuenta que aún no se cuenta con un tratamiento efectivo contra ella. Aproximadamente el 15% de los afectados con EA presentan agregación familiar, de los cuales la gran mayoría son heterocigóticos para una mutación en el gen de la Presenilina 1 (PS1 (1.

  12. 77 FR 2342 - Fifth Meeting: RTCA, Next Gen Advisory Committee

    Science.gov (United States)

    2012-01-17

    ... TRANSPORTATION Federal Aviation Administration Fifth Meeting: RTCA, Next Gen Advisory Committee AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA, NextGen... RTCA, NextGen Advisory Committee. DATES: The meeting will be held February 3, 2012, from 9:30...

  13. A genética das epilepsias

    National Research Council Canada - National Science Library

    Lopes-Cendes, Iscia

    2008-01-01

    .... Mais recentemente, estudos de genética molecular e estratégias de descoberta de genes foram usados para revelar os mecanismos moleculares e celulares envolvidas em diversas síndromes epilépticas mendelianas. SÍNTESE DOS DADOS...

  14. 25 Years of GenBank

    Science.gov (United States)

    ... to science over the last 25 years, the National Center for Biotechnology Information (NCBI), National Library of Medicine (NLM), and NIH held ... GenBank has been a critical research tool," says NCBI Director David Lipman, "enabling much of the progress ...

  15. Preserving Accuracy in GenBank

    DEFF Research Database (Denmark)

    Bidartondo, M.I.; Bruns, T. D.; Blackwell, M.

    2008-01-01

    GenBank, the public repository for nucleotide and protein sequences, is a critical resource for molecular biology, evolutionary biology, and ecology. While some attention has been drawn to sequence errors (1), common annotation errors also reduce the value of this database. In fact, for organisms...

  16. Proyección laboral de jóvenes que presentan deficiencia intelectual pertenecientes al proyecto de integración escolar municipal y escuelas especiales en la ciudad de San Felipe, Chile

    Directory of Open Access Journals (Sweden)

    Sergio Emilio Manosalva Mena

    2011-01-01

    Full Text Available El presente artículo da cuenta de una investigación que se realizó dentro de la ciudad de San Felipe (Chile, con cuatro establecimientos educacionales y empresas de la zona. De las escuelas que entregan formación laboral a jóvenes que presentan deficiencia mental, dos son escuelas especiales tradicionales y dos son liceos técnico-profesionales en la modalidad de inclusión/integración de estudiantes con y sin discapacidad. Se estimó pertinente la realización de un estudio de tipo cualitativo descriptivo, principalmente para conocer el tipo de formación que reciben estos alumnos, la demanda de mano de obra que requiere la comuna, y la percepción que posee el empresariado que emplea en sus dependencias a personas que presentan discapacidad intelectual. Los resultados de la investigación permitieron establecer que existe una descontextualización e incoherencia entre la demanda, la oferta de mano de obra y la formación laboral de los jóvenes con discapacidad intelectual en la ciudad de San Felipe.

  17. Gen IV Materials Handbook Implementation Plan

    Energy Technology Data Exchange (ETDEWEB)

    Rittenhouse, P.; Ren, W.

    2005-03-29

    A Gen IV Materials Handbook is being developed to provide an authoritative single source of highly qualified structural materials information and materials properties data for use in design and analyses of all Generation IV Reactor Systems. The Handbook will be responsive to the needs expressed by all of the principal government, national laboratory, and private company stakeholders of Gen IV Reactor Systems. The Gen IV Materials Handbook Implementation Plan provided here addresses the purpose, rationale, attributes, and benefits of the Handbook and will detail its content, format, quality assurance, applicability, and access. Structural materials, both metallic and ceramic, for all Gen IV reactor types currently supported by the Department of Energy (DOE) will be included in the Gen IV Materials Handbook. However, initial emphasis will be on materials for the Very High Temperature Reactor (VHTR). Descriptive information (e.g., chemical composition and applicable technical specifications and codes) will be provided for each material along with an extensive presentation of mechanical and physical property data including consideration of temperature, irradiation, environment, etc. effects on properties. Access to the Gen IV Materials Handbook will be internet-based with appropriate levels of control. Information and data in the Handbook will be configured to allow search by material classes, specific materials, specific information or property class, specific property, data parameters, and individual data points identified with materials parameters, test conditions, and data source. Details on all of these as well as proposed applicability and consideration of data quality classes are provided in the Implementation Plan. Website development for the Handbook is divided into six phases including (1) detailed product analysis and specification, (2) simulation and design, (3) implementation and testing, (4) product release, (5) project/product evaluation, and (6) product

  18. Safety Assurance in NextGen

    Science.gov (United States)

    HarrisonFleming, Cody; Spencer, Melissa; Leveson, Nancy; Wilkinson, Chris

    2012-01-01

    The generation of minimum operational, safety, performance, and interoperability requirements is an important aspect of safely integrating new NextGen components into the Communication Navigation Surveillance and Air Traffic Management (CNS/ATM) system. These requirements are used as part of the implementation and approval processes. In addition, they provide guidance to determine the levels of design assurance and performance that are needed for each element of the new NextGen procedures, including aircraft, operator, and Air Navigation and Service Provider. Using the enhanced Airborne Traffic Situational Awareness for InTrail Procedure (ATSA-ITP) as an example, this report describes some limitations of the current process used for generating safety requirements and levels of required design assurance. An alternative process is described, as well as the argument for why the alternative can generate more comprehensive requirements and greater safety assurance than the current approach.

  19. Esquizofrenia, genética y complejidad

    OpenAIRE

    Jensen-Pennington, Henning

    2011-01-01

    El artículo plantea la necesidad de no sobresimplificar fenómenos complejos como el de la esquizofrenia, pues su etiología implica la interacción de aspectos genéticos, biológicos y sociopsicológicos. Se plantea que el mismo conocimiento genético imposibilita la asunción de estas posiciones radicales, razón por la cual la investigación biológica actúa con cautela en la formulación de hipótesis causales. No obstante, se pone de manifiesto la interpretación inapropiada de los resultados de inve...

  20. Análisis de la diversidad genética de 21 aislamientos del hongo Moniliophthora roreri basado en marcadores RAPD

    Directory of Open Access Journals (Sweden)

    Boris Gutarra Castillo

    2013-12-01

    Full Text Available Objetivos: Estudiar la diversidad genética de 21 aislamientos del hongo que afecta al cultivo del cacao, Moniliophthora roreri, en tres zonas cacaoteras del Perú (Tocache, Mariscal Cáceres y Leoncio Prado. Métodos: Se utilizó 14 iniciadores RAPD (random amplified polymorphic DNA polimórficos y una pareja de oligonucleótidos, los que fueron empleados bajo condiciones de amplificación estandarizadas. Con los datos obtenidos se construyó un dendograma utilizando el coeficiente de Jaccard y el algoritmo UPGMA (Unweighted Pair-Group Method using Arithmetic Average. La estructura genética fue estimada en función del análisis molecular de variancia (AMOVA y la diversidad mediante los índices de Shannon y Nei. Resultados: Fueron conseguidas 59 bandas RAPD con un 73% de polimorfismo. El dendograma obtenido a un índice de similitud de 0,70, claramente dividió los individuos en tres grupos. El análisis de la diversidad genética mostró altos valores en las zonas estudiadas de acuerdo con el índice de Shannon (0,3936 y de Nei (0,2622, con mayor riqueza en Leoncio Prado. Estas zonas presentan alta variabilidad, y según el AMOVA realizado: 88% entre accesiones por zona y solo 12% entre zonas. Conclusiones: Existe más de un grupo genético de Moniliophthora roreri en la Amazonía del Perú. Estos grupos, provenientes del Ecuador, pudieron haber ingresado por el intercambio de semillas y/o de forma natural por medio de los ríos en común y estarían originando nuevos grupos genéticos locales.

  1. Standby for the Gen-Set

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1998-08-01

    Diesel Gen-Sets are hardly romantic instruments, yet they do save lives. Humming away in the corner for back-up power or emergency generation, this compact form of power supply, keeps hospital lights on, computers going and often proves a vital, if unnoticed addition to festivals and outdoor events. And as many master engine builders have discovered, this is a rapidly growing market. (author)

  2. Genética do autismo

    OpenAIRE

    Carvalheira,Gianna; VERGANI, Naja; Brunoni,Décio

    2004-01-01

    O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.

  3. Genética de comunidades

    Directory of Open Access Journals (Sweden)

    M. Verdú

    2009-01-01

    Full Text Available Los estudios sobre los procesos responsables del ensamblaje de las especies en comunidades ecológicas tradicionalmente han considerado a las especies como unidades de estudio. Sin embargo, dentro de las especies existe gran cantidad de variación genotípica y fenotípica que puede finalmente ser decisiva en el resultado de las interacciones con el resto de especies en la comunidad (competencia, mutualismo, etc. Recientemente se ha propuesto una nueva regla de ensamblaje de las comunidades que sugiere que la composición genética de una población afecta a la estructura y composición de la comunidad (es decir alfenotipo de la comunidad. Apoyando esta regla, varios estudios han demostrado que los cambios en la diversidad genética de poblaciones de plantas conllevan cambios predecibles en sus comunidades asociadas de herbívoros y predadores. Más allá de la visión poblacional, la nueva genética de comunidades pretende demostrar 1 que el genotipo particular de ciertos individuos es responsable del fenotipo de la comunidad, 2 que el fenotipo de la comunidad es heredable, y por lo tanto 3 que las comunidades evolucionan. Tras explicar estos tres conceptos, se discute el valor que esta aproximación puede tener desde el punto de vista de la biología de la conservación.

  4. Generic classification of the Archiborborinae (Diptera: Sphaeroceridae), with a revision of Antrops Enderlein, Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov.

    Science.gov (United States)

    Kits, Joel H; Marshall, Stephen A

    2013-01-01

    The Archiborborinae comprise a diverse clade of flies in the family Sphaeroceridae. We here revise the generic classification, redefining the genus Antrops Enderlein and naming 5 new genera: Boreantrops gen. nov., Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov. The genus Archiborborus, until recently a paraphyletic assemblage including most of the described species in the subfamily, is treated as a junior synonym of Antrops (syn. nov.) We revise the genera Antrops (53 species, including 40 sp. nov.: Antrops anovariegatus, Antrops aurantifemur, Antrops baeza, Antrops bellavista, Antrops biflavus, Antrops bucki, Antrops carpishensis, Antrops cochabamba, Antrops cochinoca, Antrops coniobaptos, Antrops coroico, Antrops cotopaxi, Antrops didactylos, Antrops diversipennis, Antrops eurus, Antrops fulgiceps, Antrops fuliginosus, Antrops guandera, Antrops guaramacalensis, Antrops inca, Antrops juninensis, Antrops mucarensis, Antrops niger, Antrops papallacta, Antrops pecki, Antrops podocarpus, Antrops quadrilobus, Antrops siberia, Antrops sierrazulensis, Antrops tachira, Antrops tequendama, Antrops tetrastichus, Antrops tumbrensis, Antrops unduavi, Antrops variegatus, Antrops versabilis, Antrops vittatus, Antrops yungas, and Antrops zongo and the following comb. nov.: Antrops annulatus (Richards), Antrops chaetosus (Richards), Antrops femoralis (Blanchard), Antrops hirtus (Bigot), Antrops maculipennis (Duda), Antrops maximus (Richards), Antrops microphthalmus (Richards), Antrops quadrinotus (Bigot), Antrops setosus (Duda), Antrops simplicimanus (Richards), Antrops nitidicollis (Becker), and Antrops orbitalis (Duda)), Coloantrops (1 species: Coloantrops daedalus, sp. nov.), Maculantrops (2 species, Maculantrops hirtipes (Macquart) comb. nov. and Maculantrops altiplanus, sp. nov.), Photoantrops (1 species: Pho-toantrops echinus sp. nov.), and Poecilantrops (10 species: Poecilantrops baorucensis, Poecilantrops boraceiensis

  5. Testigos modestos y poblaciones invisibles en la cobertura de la genética humana en los medios de comunicación colombianos

    Directory of Open Access Journals (Sweden)

    Adriana Díaz del Castillo

    2012-06-01

    Full Text Available En este artículo exploramos cómo los medios de comunicación colombianos configuran la práctica de la genética humana, reproduciendo ciertos discursos estandarizados sobre ciencia que se encuentran atravesados por el género y la raza. Para este fin llevamos a cabo un análisis etnográfico de la cobertura de la genética humana (poblacional y forense en dos de los medios de mayor penetración en el país en los periodos de 1992-2006 (periódico y 2009-2010 (periódico y noticiero. Nuestro argumento es que por una parte, los medios de comunicación presentan a la ciencia genética como única/uniforme/objetiva/neutra/heroica. Por otro lado, presenta sujetos que son simultáneamente marcados y desmarcados en términos de raza, género, clase y geografía. Concluimos que este último mecanismo reproduce y moviliza la idea del mestizaje como una ideología de construcción de nación.

  6. Testigos modestos y poblaciones invisibles en la cobertura de la genética humana en los medios de comunicación colombianos

    Directory of Open Access Journals (Sweden)

    Adriana Díaz del Castillo

    Full Text Available En este artículo exploramos cómo los medios de comunicación colombianos configuran la práctica de la genética humana, reproduciendo ciertos discursos estandarizados sobre ciencia que se encuentran atravesados por el género y la raza. Para este fin llevamos a cabo un análisis etnográfico de la cobertura de la genética humana (poblacional y forense en dos de los medios de mayor penetración en el país en los periodos de 1992-2006 (periódico y 2009-2010 (periódico y noticiero. Nuestro argumento es que por una parte, los medios de comunicación presentan a la ciencia genética como única/uniforme/objetiva/neutra/heroica. Por otro lado, presenta sujetos que son simultáneamente marcados y desmarcados en términos de raza, género, clase y geografía. Concluimos que este último mecanismo reproduce y moviliza la idea del mestizaje como una ideología de construcción de nación.

  7. Das seneszenzassoziierte Gen HvS40 der Gerste

    OpenAIRE

    Trösch, Mirl

    2016-01-01

    In der vorliegenden Arbeit wurde das seneszenzassoziierte Gen HvS40 der Gerste als dual kodierendes Gen charakterisiert. Damit wurde ein solches Gen erstmals in Pflanzen beschrieben. Der alternative S40+1-Leserahmen, der den kanonischen Leserahmen im 5'-Bereich überragt, konnte auch in anderen monokotylen, jedoch nicht in dikotylen Arten gefunden werden. Das S40-Protein, das durch den kanonischen Leserahmen S40+3 kodiert wird, kann der pflanzenspezifischen Proteinfamilie DUF584 zugeordnet...

  8. Aconselhamento genético Genetic counseling

    Directory of Open Access Journals (Sweden)

    João Monteiro de Pina-Neto

    2008-08-01

    Full Text Available OBJETIVO: Esta revisão sobre aconselhamento genético (AG teve o objetivo de mostrar os conceitos atuais e os princípios filosóficos e éticos aceitos na grande maioria dos países e recomendados pela Organização Mundial da Saúde, as fases do processo, seus resultados e o impacto psicológico de uma doença genética em uma família. FONTES DOS DADOS: Os conceitos apresentados são baseados em uma síntese histórica da literatura sobre AG desde a década de 1930 até o momento atual, sendo que os artigos citados representam os principais trabalhos publicados e que hoje fundamentam a teoria e a prática do AG. SÍNTESE DOS DADOS: O AG modernamente é definido como um processo de comunicação que trata dos problemas humanos relacionados à ocorrência de uma doença genética em uma família. É fundamental que os profissionais da saúde conheçam os aspectos psicológicos desencadeados pela doença genética e como estes aspectos podem ser manejados. Vivemos ainda na genética humana e médica uma fase de predomínio dos aspectos técnicos e científicos e de pouca ênfase no estudo das reações emocionais e dos processos de adaptação das pessoas a estas doenças, o que leva ao baixo entendimento dos clientes sobre os fatos ocorridos, com conseqüências negativas sobre a vida familiar e para a sociedade. CONCLUSÕES: Conclui-se pela necessidade de que as famílias com doenças genéticas sejam encaminhadas para AG e que os profissionais desta área invistam mais na humanização do atendimento, desenvolvendo mais as técnicas do AG psicológico não-diretivo.OBJECTIVE: The objective of this review of genetic counseling (GC is to describe the current concepts and philosophical and ethical principles accepted by the great majority of countries and recommended by the World Health Organization, the stages of the process, its results and the psychological impact that a genetic disease has on a family. SOURCES: The concepts presented are

  9. TidGen Power System Commercialization Project

    Energy Technology Data Exchange (ETDEWEB)

    Sauer, Christopher R. [President & CEO; McEntee, Jarlath [VP Engineering & CTO

    2013-12-30

    ORPC Maine, LLC, a wholly-owned subsidiary of Ocean Renewable Power Company, LLC (collectively ORPC), submits this Final Technical Report for the TidGen® Power System Commercialization Project (Project), partially funded by the U.S. Department of Energy (DE-EE0003647). The Project was built and operated in compliance with the Federal Energy Regulatory Commission (FERC) pilot project license (P-12711) and other permits and approvals needed for the Project. This report documents the methodologies, activities and results of the various phases of the Project, including design, engineering, procurement, assembly, installation, operation, licensing, environmental monitoring, retrieval, maintenance and repair. The Project represents a significant achievement for the renewable energy portfolio of the U.S. in general, and for the U.S. marine hydrokinetic (MHK) industry in particular. The stated Project goal was to advance, demonstrate and accelerate deployment and commercialization of ORPC’s tidal-current based hydrokinetic power generation system, including the energy extraction and conversion technology, associated power electronics, and interconnection equipment capable of reliably delivering electricity to the domestic power grid. ORPC achieved this goal by designing, building and operating the TidGen® Power System in 2012 and becoming the first federally licensed hydrokinetic tidal energy project to deliver electricity to a power grid under a power purchase agreement in North America. Located in Cobscook Bay between Eastport and Lubec, Maine, the TidGen® Power System was connected to the Bangor Hydro Electric utility grid at an on-shore station in North Lubec on September 13, 2012. ORPC obtained a FERC pilot project license for the Project on February 12, 2012 and the first Maine Department of Environmental Protection General Permit issued for a tidal energy project on January 31, 2012. In addition, ORPC entered into a 20-year agreement with Bangor Hydro Electric

  10. Expresión del gen esp (enterococcus surface protein de enterococcus faecalis en un modelo in vitro de dientes extraídos

    Directory of Open Access Journals (Sweden)

    E. Covo Morales

    Full Text Available Objetivo: Determinar la presencia y expresión del gen esp en cepas clínicas de Enterococcus faecalis a partir de un modelo in vitro de dientes extraídos. Métodos: Se diseñó un sistema in vitro para evaluar la formación del biofilm mediante microscopía de fluorescencia y la expresión del gen esp. El sistema estuvo constituido por un diente humano previamente extraído, cortado y preparado que proporcionó, mediante su conducto radicular, una superficie adecuada para la formación del biofilm por parte de E. faecalis. El sistema dispuso de una cámara anaerobia que permitió el crecimiento de la bacteria en el caldo de cultivo y evitó su contaminación con otros microorganismos. Esta cámara estuvo constituida por un tubo de micro centrifuga estéril, cortado y unido por el extremo inferior al extremo apical del diente seccionado. Resultados: Los resultados que se obtuvieron tanto por microscopia de fluorescencia como por RT-PCR permitieron cuantificar el nivel de expresión del gen esp en las bacterias durante su crecimiento y formando el biofilm en la superficie de los conductos radiculares. Todas las cepas evaluadas presentan el gen esp. Sin embargo, el biofilm de la cepa CC02 expresó el gen esp cuatro veces más en comparación al gen esp de la cepa de referencia. Conclusión: La expresión del gen esp podría estar asociada con la formación de biofilm en E. faecalis y la adherencia a superficies abióticas. Podría convertirse en una diana terapéutica prometedora en los programas de control de infecciones persistentes por Enterococcus spp. asociados a la presencia de biofilm.

  11. Genética molecular del alcoholismo

    OpenAIRE

    Mauricio Rey-Buitrago

    2015-01-01

    El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a to...

  12. Gen IV Materials Handbook Functionalities and Operation

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Weiju [ORNL

    2009-12-01

    This document is prepared for navigation and operation of the Gen IV Materials Handbook, with architecture description and new user access initiation instructions. Development rationale and history of the Handbook is summarized. The major development aspects, architecture, and design principles of the Handbook are briefly introduced to provide an overview of its past evolution and future prospects. Detailed instructions are given with examples for navigating the constructed Handbook components and using the main functionalities. Procedures are provided in a step-by-step fashion for Data Upload Managers to upload reports and data files, as well as for new users to initiate Handbook access.

  13. Autismo: genética Autism: genetics

    Directory of Open Access Journals (Sweden)

    Abha R Gupta

    2006-05-01

    Full Text Available O autismo é um transtorno fortemente genético, com uma herdabilidade estimada de mais de 90%. Uma combinação de heterogeneidade fenotípica e o provável envolvimento de múltiplos loci que interagem entre si dificultam os esforços de descobertas de genes. Conseqüentemente, a etiologia genética dos transtornos relacionados ao autismo permanece, em grande parte, desconhecida. Nos últimos anos, a convergência entre tecnologias genômicas em rápido avanço, a finalização do projeto genoma humano e os crescentes e exitosos esforços em colaboração para aumentar o número de pacientes disponíveis para estudo conduziram às primeiras pistas sólidas sobre as origens biológicas desses transtornos. Este artigo revisará a literatura até nossos dias, resumindo os resultados de estudos de ligação genética, citogenéticos e de genes candidatos com um foco no progresso recente. Além disso, são consideradas as vias promissoras para pesquisas futuras.Autism is a strongly genetic disorder, with an estimated heritability of greater than 90%. A combination of phenotypic heterogeneity and the likely involvement of multiple interacting loci have hampered efforts at gene discovery. As a consequence, the genetic etiology of the spectrum of autism related disorders remains largely unknown. Over the past several years, the convergence of rapidly advancing genomic technologies, the completion of the human genome project, and increasingly successful collaborative efforts to increase the number of patients available for study have led to the first solid clues to the biological origins of these disorders. This paper will review the literature to date summarizing the results of linkage, cytogenetic, and candidate gene studies with a focus on recent progress. In addition, promising avenues for future research are considered.

  14. Genética molecular del alcoholismo

    Directory of Open Access Journals (Sweden)

    Mauricio Rey-Buitrago

    2015-07-01

    Full Text Available El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a todo el genoma, de expresión y epigenéticos han identificado una amplia gama de variantes genéticas y epigenéticas, blancos para los estudios de susceptibilidad, diagnóstico y tratamiento farmacológico. Actualmente se comprenden mucho más estas relaciones y el desarrollo rápido de nuevas metodologías de estudio promete continuar este proceso, así como la generación de algoritmos de diagnóstico, prevención y tratamientos más acertados y confiables.

  15. Towards an International Culture: Gen Y Students and SNS?

    Science.gov (United States)

    Lichy, Jessica

    2012-01-01

    This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

  16. Gen-duplikationer - evolutionens eksperimentarium med et eksempel fra hvede

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh; Dionisio, Giuseppe; Brinch-Pedersen, Henrik

    2013-01-01

    Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag.......Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag....

  17. Towards an International Culture: Gen Y Students and SNS?

    Science.gov (United States)

    Lichy, Jessica

    2012-01-01

    This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

  18. Gen-duplikationer - evolutionens eksperimentarium med et eksempel fra hvede

    DEFF Research Database (Denmark)

    Madsen, Claus Krogh; Dionisio, Giuseppe; Brinch-Pedersen, Henrik

    2013-01-01

    Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag.......Den nyeste forskning i kornarternes fytaser viser, hvordan en gen-duplikation for 32-54 mio. år siden påvirker foderkvaliteten i dag....

  19. Biometris GenStat Procedure Library Manual 12th Edition

    NARCIS (Netherlands)

    Goedhart, P.W.; Thissen, J.T.N.M.

    2009-01-01

    The Biometris GenStat Procedure Library contains procedures in which new and existing statistical methodology is implemented, as well as procedures to make GenStat more user-friendly. The Biometris library can be used and distributed freely. The Library is standardly distributed over the Dutch

  20. Biometris GenStat Procedure Library Manual 13th Edition

    NARCIS (Netherlands)

    Goedhart, P.W.; Thissen, J.T.N.M.

    2010-01-01

    The Biometris GenStat Procedure Library contains procedures in which new and existing statistical methodology is implemented, as well as procedures to make GenStat more user-friendly. The Biometris library can be used and distributed freely. The Library is standardly distributed over the Dutch

  1. 78 FR 8108 - NextGen Solutions Vendors Guide

    Science.gov (United States)

    2013-02-05

    ... Organization's (ICAO) Aviation System Block Upgrade (ASBU) initiative. The NextGen solutions address the ICAO... help implement ICAO-consistent NextGen solutions. The guide will highlight the U.S. producers and... to aviation system upgrades) Example: Engineering Services More information on the four ICAO...

  2. GenCade Version 1 Quick-Start Guide: How to Start a Successful GenCade Project

    Science.gov (United States)

    2015-03-01

    Properly defining the inlets is a crucial part of a GenCade project and can be difficult. A user should become familiar with the Inlet Reservoir Model ( IRM ...GenCade Report 2 provide additional documentation on IRM variable names and functions. 3.9.4 Export data The data may easily be exported to a text

  3. Gen-Umwelt-Interaktionen und Gen-Umwelt-Korrelationen bei psychiatrischen Erkrankungen

    Directory of Open Access Journals (Sweden)

    Winkler D

    2010-01-01

    Full Text Available Die seit Langem bestehende Frage, in welchem Ausmaß Anlage und Umwelt zu psychologischen Merkmalen und psychiatrischen Erkrankungen beitragen, wird durch Ergebnisse von Zwillingsstudien und in letzter Zeit durch Untersuchungen des Zusammenhangs zwischen molekulargenetischen Merkmalen und Umwelteinflüssen bereichert. Eine Gen-Umwelt-Interaktion liegt dann vor, wenn genetische Faktoren die Auswirkungen von Umweltbedingungen modulieren. Die Genetik kann weiters die Wahrscheinlichkeit der Exposition gegenüber bestimmten Umwelteinflüssen verändern, was als Gen- Umwelt-Korrelation bezeichnet wird. Beide Phänomene liegen aber häufig gleichzeitig vor, was eine besondere Herausforderung für die Konzeption von wissenschaftlichen Studien darstellt.

  4. Delineamento de experimentos em genética genômica Experimental design in genetical genomics

    OpenAIRE

    Guilherme Jordão de Magalhães Rosa

    2007-01-01

    Genética genômica é um termo utilizado para representar o estudo de processos genéticos controladores de caracteres fenotípicos de herança complexa, a partir da análise conjunta de informação relativa a fenótipos, estruturas de parentesco, marcadores moleculares e expressão gênica. Estudos de genética genômica são utilizados, por exemplo, para a estimação da herdabilidade de níveis de transcrição, para o mapeamento de locos controladores da expressao gênica (eQTL, do inglês expression Quantit...

  5. Transferencia genética en Plantas

    OpenAIRE

    Carbonero Zalduegui, Pilar; García Olmedo, Francisco

    1985-01-01

    La mejora genética de las especies vegetales cultivadas ha sido, y continuará siendo, una de las armas más decisivas en la lucha permanente por mantener los incrementos de la producción de alimentos por delante del crecimiento de la población humana. Puede afirmarse que el hombre ha practicado de un modo empírico la mejora vegetal desde que, en los albores de la revolución agrícola, hace alrededor de 10.000 años, inició su gradual conversión de cazador-recolector en agricultor. Sin embargo, e...

  6. NextGen Future Safety Assessment Game

    Science.gov (United States)

    Ancel, Ersin; Gheorghe, Adrian; Jones, Sharon Monica

    2011-01-01

    The successful implementation of the next generation infrastructure systems requires solid understanding of their technical, social, political and economic aspects along with their interactions. The lack of historical data that relate to the long-term planning of complex systems introduces unique challenges for decision makers and involved stakeholders which in turn result in unsustainable systems. Also, the need to understand the infrastructure at the societal level and capture the interaction between multiple stakeholders becomes important. This paper proposes a methodology in order to develop a holistic approach aiming to provide an alternative subject-matter expert (SME) elicitation and data collection method for future sociotechnical systems. The methodology is adapted to Next Generation Air Transportation System (NextGen) decision making environment in order to demonstrate the benefits of this holistic approach.

  7. Innatismo y control genético

    Directory of Open Access Journals (Sweden)

    Sergio Daniel Barberis

    2013-12-01

    Full Text Available Mi objetivo en este artículo es defender una elucidación reduccionista parcial del concepto de lo innato en ciencias cognitivas. En primer lugar, especifico las condiciones mínimas para una elucidación de lo innato. En segundo lugar, presento los principales enfoques que se han adoptado en la discusión, es decir, las propuestas reduccionistas, autonomistas y escépticas. Luego de ofrecer argumentos versus el escepticismo y las propuestas autonomistas, presento en detalle lo que considero es una elucidación parcial de lo innato en términos de control genético, mostrando cómo un concepto de la biología del desarrollo puede ser provechosamente utilizado para elucidar el concepto de lo innato en ciencias cognitivas. Por último, explicito las ventajas filosóficas de mi propuesta.

  8. RxGen General Optical Model Prescription Generator

    Science.gov (United States)

    Sigrist, Norbert

    2012-01-01

    RxGen is a prescription generator for JPL's in-house optical modeling software package called MACOS (Modeling and Analysis for Controlled Optical Systems), which is an expert optical analysis software package focusing on modeling optics on dynamic structures, deformable optics, and controlled optics. The objectives of RxGen are to simplify and automate MACOS prescription generations, reducing errors associated with creating such optical prescriptions, and improving user efficiency without requiring MACOS proficiency. RxGen uses MATLAB (a high-level language and interactive environment developed by MathWorks) as the development and deployment platform, but RxGen can easily be ported to another optical modeling/analysis platform. Running RxGen within the modeling environment has the huge benefit that variations in optical models can be made an integral part of the modeling state. For instance, optical prescription parameters determined as external functional dependencies, optical variations by controlling the in-/exclusion of optical components like sub-systems, and/or controlling the state of all components. Combining the mentioned capabilities and flexibilities with RxGen's optical abstraction layer completely eliminates the hindering aspects for requiring proficiency in writing/editing MACOS prescriptions, allowing users to focus on the modeling aspects of optical systems, i.e., increasing productivity and efficiency. RxGen provides significant enhancements to MACOS and delivers a framework for fast prototyping as well as for developing very complex controlled optical systems.

  9. Las mutaciones inestables, nuevo reto para el consejo genético de enfermedades hereditarias

    Directory of Open Access Journals (Sweden)

    Patricia Cuenca

    2004-09-01

    Full Text Available Las mutaciones inestables constituyen un tipo de alteración genética descubierta en la década de los noventa. En condiciones normales, regiones específicas de los genes están constituidas por repeticiones de una secuencia corta que puede ser de tres, cuatro, cinco o más nucleótidos; por ejemplo CAG, CGG, ATTCT, etc. Este nuevo tipo de mutación consiste en un aumento en la cantidad de éstas repeticiones, lo que causa una alteración en la expresión de dichos genes. Son inestables porque se ha observado que el tamaño de la secuencia repetida varía cuando las células se dividen por mitosis o meiosis, lo cual tiene implicaciones sobre la herencia y por consiguiente sobre el consejo genético que debe brindarse a los afectados. Estas mutaciones se han encontrado en genes importantes para la función neurológica normal del ser humano, donde pueden alterar el transporte de los ARN desde el núcleo al citoplasma, provocar la inactivación del gen o producir una proteína con funciones nuevas. La mayoría de las enfermedades causadas por estas mutaciones afectan el sistema nervioso, son degenerativas y presentan el fenómeno de anticipación genética, es decir que los afectados dentro de una familia se enfermarán más jóvenes y en forma más severa con el paso de las generaciones. La cantidad de repeticiones de la secuencia repetida presenta una correlación negativa con la edad de manifestación, así como una correlación positiva con la severidad de la enfermedad. En este momento existen veinte padecimientos identificados que son causados exclusivamente por este tipo de mutación. Este trabajo es una breve revisión actualizada del tema.Unstable mutations, new challenges for genetic counseling of inherited disorders. Unstable mutations or amplification of DNA tandem repeats sequences constitute a new kind of genetic alteration discovered in the 90´s that cause hereditary diseases. This mutation has been found inside or near important

  10. Modelo poblacional con algoritmos genéticos

    OpenAIRE

    Veliz Quintero, Eduardo; Rodriguez Ojeda, Luis

    2009-01-01

    Para el desarrollo de este trabajo, “MODELO POBLACIONAL CON ALGORITMOS GENÉTICOS”, he investigado la rama de la inteligencia artificial, como son los algoritmos genéticos. Primero presento en forma general los aspectos que envuelven los algoritmos genéticos, parto de la necesidad de optimizar, así como su historia y posibles aplicaciones y luego he cubierto detalladamente todo lo que pude investigar sobre la teoría de los algoritmos genéticos, sus fundamentos matemáticos, tipos de algoritmos ...

  11. Genética forense no-humana

    OpenAIRE

    Peral García, Pilar; Giovambattista, Guillermo; Ripoli, María Verónica

    2015-01-01

    El presente libro nace como producto de una convocatoria para Libros de Cátedra de la Universidad Nacional de la Plata. La propuesta, avalada por la Facultad de Ciencias Veterinarias, permitirá introducir al lector en los principales aspectos de la identificación genética de animales y/o sus productos derivados, conocer los principales métodos de genotipificación utilizados en genética forense, reconocer los lineamientos de estandarización y acreditación de los laboratorios de genética forens...

  12. Identificación en lazo cerrado mediante algoritmos genéticos

    Directory of Open Access Journals (Sweden)

    Alfredo Gómez Infante

    2010-11-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 st1:*{behavior:url(#ieooui } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} La identificación  en lazo cerrado de sistemas continuos es un problema de optimización no lineal que resulta de difícil solución mediante métodos convencionales.  En este trabajo se propone el uso de algoritmos genéticos (AG para esta tarea y se muestra mediante simulaciones que los modelos obtenidos pueden reproducir fielmente el comportamiento de la planta, aun en el caso de sistemas inestables o que presentan algún tipo de no-linealidad. Estos modelos pueden utilizarse para el ajuste de los parámetros de reguladores PID utilizando un método también basado en AG, como se muestra en dos ejemplos: un sistema inestable en lazo cerrado y otro de alto orden con retardo.  La metodología propuesta se aplica también  a la identificación del modelo AC5A de la IEEE, que incluye una no-linealidad del tipo saturación,  para unidades de generación eléctrica.

  13. GenBank blastx search result: AK062102 [KOME

    Lifescience Database Archive (English)

    Full Text Available transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608; aspartic

  14. GenBank blastx search result: AK242621 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  15. GenBank blastx search result: AK243655 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  16. EPCGen2 Pseudorandom Number Generators: Analysis of J3Gen

    Directory of Open Access Journals (Sweden)

    Alberto Peinado

    2014-04-01

    Full Text Available This paper analyzes the cryptographic security of J3Gen, a promising pseudo random number generator for low-cost passive Radio Frequency Identification (RFID tags. Although J3Gen has been shown to fulfill the randomness criteria set by the EPCglobal Gen2 standard and is intended for security applications, we describe here two cryptanalytic attacks that question its security claims: (i a probabilistic attack based on solving linear equation systems; and (ii a deterministic attack based on the decimation of the output sequence. Numerical results, supported by simulations, show that for the specific recommended values of the configurable parameters, a low number of intercepted output bits are enough to break J3Gen. We then make some recommendations that address these issues.

  17. Pertinencia de las técnicas de laboratorio para el diagnóstico de enfermedades genéticas Pertinence of the lab techniques for the diagnosis of genetic diseases

    Directory of Open Access Journals (Sweden)

    Estela Morales Peralta

    2008-06-01

    Full Text Available Las pruebas genéticas son métodos de laboratorio que permiten identificar alteraciones de proteínas, ciertos metabolitos, cromosomas o ácidos nucleicos relacionados con enfermedades de causa genética. Constituyen técnicas de avanzada que llegan a revelar las bases moleculares de estas afecciones. Sin embargo, no son el método de referencia para el diagnóstico, pues no siempre presentan sensibilidad y especificidad total. Además, existe un grupo de enfermedades genéticas, cuyo diagnóstico se puede confirmar sobre la base de estudios complementarios que no son precisamente pruebas genéticas; mientras otras requieren solo los criterios clínicos tradicionales para ser verificadas. Las pruebas genéticas complementan el diagnóstico, pero no lo realizan. El proceso de diagnóstico es el método clínico, variante del científico experimental, actividad humana en la que el razonamiento médico es lo esencial.The genetic tests are lab methods that allow to identify protein alterations, certain metabolites, chromosomes or nucleic acids connected with diseases of genetic cause. They are advanced techniques that reveal the molecular bases of these affections. However, they are not the reference method for the diagnosis, since they do not always present total sensitivity and specificity. Moreover, there is a group of genetic diseases, whose diagnosis may be confirmed on the basis of complementary studies that are not precisely genetic tests; whereas other require only traditional clinical criteria to be confirmed. The diagnostic process is the clinical method, a variant of the scientific experimental method, a human activity in which medical reasoning is essential.

  18. Psychrophilic sulfate-reducing bacteria isolated from permanently cold Arctic marine sediments: description of Desulfofrigrus oceanense gen. nov., sp nov., Desulfofrigus fragile sp nov., Desulfofaba gelida gen. nov., sp nov., Desulfotalea psychrophila gen. nov., sp nov and Desulfotalea arctica sp

    DEFF Research Database (Denmark)

    Knoblauch, C.; Sahm, K.; Jørgensen, BB

    1999-01-01

    known sulfate reducers. Due to the phylogenetic and phenotypic differences between the new isolates and their closest relatives, establishment of the new genera Desulfotalea gen. nov., Desulfofaba gen. nov. and Desulfofrigus gen. nov. is proposed, with strain ASv26(T) as the type strain of the type...

  19. Equilibrium analysis in imperfect Traders' and GenCos' market

    OpenAIRE

    Zhong, J; Chitkara, P.

    2010-01-01

    The paper models the strategic behavior of traders, GenCos and ISO using the multi-leader-follower framework. The outcomes of the strategic behavior of the players have been modeled using an equilibrium problem with equilibrium constraints. From a policy perspective it is seen that allowing the GenCos to hold FTRs may be welfare enhancing under certain demand conditions and ownership patterns of transmission rights and generation assets. The proposed model has been simulated on a 3 bus system...

  20. Introducing AstroGen: The Astronomy Genealogy Project

    OpenAIRE

    Tenn, Joseph S.

    2016-01-01

    The Astronomy Genealogy Project ("AstroGen"), a project of the Historical Astronomy Division of the American Astronomical Society (AAS), will soon appear on the AAS website. Ultimately, it will list the world's astronomers with their highest degrees, theses for those who wrote them, academic advisors (supervisors), universities, and links to the astronomers or their obituaries, their theses when on-line, and more. At present the AstroGen team is working on those who earned doctorates with ast...

  1. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus)

    OpenAIRE

    Raden Roro Sri Pudji Sinarni Dewi; Alimuddin Alimuddin; Agus Oman Sudrajat; Komar Sumantadinata; Erma Primanita Hayuningtyas

    2016-01-01

    Penelitian ekspresi sementara (transient expression) dari transgen secara in vivo menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein). Pengamatan gen EGFP (enhanced green fluorescent protein) pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus) ditujukan untuk mendapatkan informasi mengenai kema...

  2. Banque Cantonale de Genève

    CERN Multimedia

    Banque Cantonale de Genève

    2011-01-01

    7e Salon Immobilier BCGE le samedi 3 septembre 2011, de 8 h 30 à 13 h 00, au Centre de formation de Conches À cette occasion, les meilleurs spécialistes professionnels genevois de l’immobilier seront réunis en un seul et même lieu. Si vous le souhaitez, un conseiller spécialisé dans les financements hypothécaires évaluera vos possibilités d’investissement immobilier adaptées à votre situation personnelle. En parallèle, les plus importantes régies immobilières de Genève seront à votre disposition pour vous présenter leurs offres actuelles, ainsi que les projets immobiliers futurs et discuter avec vous de la meilleure stratégie à adopter pour trouver l’objet de vos rêves. De plus, vous aurez la possibilité...

  3. Identidad y Eponimia genérica

    Directory of Open Access Journals (Sweden)

    Cristina Virgen Aldana-Zayas

    2013-01-01

    Full Text Available Se aborda el tema de la Eponimia genérica de la flora vascular cubana que comprende el nombre dado a cada género de las plantas superiores y su relación con la formación identitaria en los estudiantes. Para ello se realizó una revisión bibliográfica de los cinc o tomos de la flora cubana y Árboles de Cuba. Se tomó una muestra de 331 géneros. Además se utilizaron otras fuentes bibliográficas para establecer los nexos entre la lingüística, la identidad y los nombres de los géneros de la flora vascular cubana. El empleo del método histórico-lógico permitió constatar que la procedencia de los nombres de los géneros puede deberse a la mitología, a la geografía y a nombres de científicos. Para la investigación se seleccionaron los nombres de los géneros dedicados a personalidades de la ciencia. Se confeccionó un Catálogo que ofrece las herramientas necesarias para que profesores y estudiantes realicen sus propias indagaciones y lleguen a conclusiones sobre los epónimos y sus potencialidades identitarias.

  4. New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales).

    Science.gov (United States)

    Smith, Matthew E; Amses, Kevin R; Elliott, Todd F; Obase, Keisuke; Aime, M Catherine; Henkel, Terry W

    2015-12-01

    Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera.

  5. Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov.--two new middle Cambrian hyolithids (?Mollusca) from the Czech Republic.

    Science.gov (United States)

    Valent, Martin; Fatka, Oldřich; Szabad, Michal; Micka, Václav; Marek, Ladislav

    2015-08-28

    Two new endemic genera and species of extinct group of Hyolitha, Skryjelites auritus gen. et sp. nov. and Quasimolites quasimodo gen. et sp. nov. are described and illustrated from the Buchava Formation of the Barrandian area (Czech Republic).

  6. SEQ-GEN: A comprehensive multimission sequencing system

    Science.gov (United States)

    Salcedo, Jose; Starbird, Thomas J.

    1994-01-01

    SEQ-GEN is a user-interactive computer program used to plan and generate a sequence of commands for spacecraft. Desired activities are specified by the user of SEQ-GEN; SEQ-GEN in turn expands these activities, deriving the spacecraft commands necessary to accomplish the desired activities. SEQ-GEN models the effects on the spacecraft of the commands, predicting the state as a function of time, flagging any conflicts and rule violations. These states, conflicts, and violations are viewable both graphically and textually at the user's request. SEQ-GEN also displays the entire sequence graphically, showing each requested activity as a bar on its graphical timeline. SEQ-GEN immediately revalidates the sequence, updating its models and calculations along with its displays based on these changes. Because it has the ability to recalculate spacecraft states immediately, the user is able to perform 'what-if' sessions easily. SEQ-GEN, a multimission tool, is adaptable to any flight project. A flight project writes its adaptation files containing project unique information including in its simplest form, only spacecraft commands. For more involved projects the adaptation files may also contain flight and mission rules, description of the spacecraft and ground models, and the definition of activities. SEQ-GEN operates at whatever level of detail the adaptation files imply. Simple adaptations are straight forward to do. There is, however, no limit to the complexity of activity definitions or of spacecraft models: both may involve unlimited logical decision points. Commands and activities may involve any number of parameters of a wide variety of data types, including integer, float, time, boolean, and character strings. SEQ-GEN will be used by the Mars Pathfinder, Cassini, and VIM (Voyager Interstellar Mission) project in an effort to speed up adaptation time and to keep sequence generation costs down. SEQ-GEN is hosted on UNIX workstations. It uses MOTIF and X for windowing

  7. Veinte años del Régimen Andino de Acceso a Recursos Genéticos

    Directory of Open Access Journals (Sweden)

    Mónica Ribadeneira Sarmiento

    2017-05-01

    Full Text Available El artículo buscar contribuir al conocimiento del régimen legal andino de acceso a recursos genéticos, cuyo instrumento central es la Decisión Andina 391, promulgada en 1996. Se analiza la norma y se presentan sus más evidentes virtudes y limitaciones. Se incluye información sobre la evolución nacional de la decisión en cada uno de los países andinos. El artículo contiene un apartado referido al futuro de la norma en el actual escenario de reingeniería del Sistema Andino de Integración (SAI. Se han consultado documentos, fuentes oficiales, artículos de expertos y entrevistas. El artículo se estructura de la siguiente manera: los orígenes de la norma; el análisis y contenido de la norma andina; el proceso de reglamentación en los países andinos; las virtudes y limitaciones; la coyuntura y el futuro, y, por último, las conclusiones.

  8. Producción científica española en el área de Genética

    Directory of Open Access Journals (Sweden)

    Martín Moreno, Carmen

    1996-12-01

    Full Text Available The aim of this bibliometric study is to find out the state of Spanish research in Biomedical Genetics at the national and international level. Documents published by Spanish geneticians were collected during 1989-1995, from two different databases: MEDLINE as an international database and IME as a national one. The Gain in Competitiveness of the Spanish Genetics versus world production, as well as versus big producer countries was calculated. The use of information was studied through the analysis of references from the articles by Spanish geneticians, their half life and Price's Index. The result s obtained show that Genetics is in a period of expansion worldwide. Big differences exist between Spanish production and the production of those countries that may be considered as big producers. Nevertheless, a clear growing trend of Spanish Genetics in national and international sources was observed.

    Con este estudio bibliométrico se ha intentado conocer la situación que ocupa la investigación española en Genética biomédica a nivel internacional, así como dentro del contexto nacional. Para ello se han estudiado los trabajos publicados por los genetistas españoles entre 1989 y 1995, recogidos por dos bases de datos biomédicas (MEDLINE e IME. Se ha estudiado la ganancia en competitividad de la Genética española frente a la producción mundial en Genética, así como a la de otros países considerados grandes productores. Asimismo, se ha realizado un estudio del consumo de información, analizando las referencias de los artículos publicados por parte de los genetistas españoles, para conocer la vida media y el índice de Price de la literatura que utilizan. Los resultados obtenidos indican que la Genética biomédica está en un momento de expansión a nivel mundial. En cuanto a la situación española se han observado grandes diferencias respecto a la producción que presentan los países considerados grandes productores. A pesar

  9. Enfoque genómico en la enfermedad cardiovascular

    Directory of Open Access Journals (Sweden)

    Ismael San Mauro-Martín

    Full Text Available Introducción: las enfermedades cardiovasculares (EC constituyen la principal causa de muerte a nivel mundial. La etiología es multifactorial, pueden influir diversos factores como la dieta, los hábitos de vida, el nivel de ejercicio físico o la carga genética. El gran número de genes implicados, así como sus diversas variantes, pueden influir sobre el riesgo de padecer enfermedades cardiovasculares por medio de distintas vías. Objetivo: determinar la relación existente entre diferentes polimorfismos genéticos y el riesgo individual de EC en población infantil y adulta. Métodos: se llevó a cabo una búsqueda bibliográfica utilizando la base de datos PubMed. La búsqueda se limitó a un periodo de diez años y a metaanálisis realizados en humanos. Resultados: se establece relación entre el riesgo de enfermedad cardiovascular y los siguientes polimorfismos genéticos: cromosoma 9p21, apolipoproteína A5, apolipoproteínas E2, E3 y E4, gen PPARG o PPARΥ, genes implicados en el metabolismo lipídico, gen MTHFR, citocromo P450, factor V de coagulación o factor de Leiden (FVL y gen VKORC. Conclusiones: Se han identificado un gran número de genes relacionados con la enfermedad cardiovascular. La carga genética puede influir de manera directa o indirecta sobre el riesgo cardiovascular, modificando factores de riesgo para enfermedad cardiovascular o actuando sobre la medicación empleada para tratarla.

  10. ANALISIS GEN HAEMAGGLUTININ PADA VIRUS CAMPAK LIAR

    Directory of Open Access Journals (Sweden)

    Subangkit Subangkit

    2015-05-01

    Full Text Available AbstrakPenyakit Campak disebabkan oleh virus campak yang termasuk genus Morbilivirus dan Family Paramyxoviridae. Penyakit campak masih menjadi masalah kesehatan karena masih ditemukan Kejadian Luar Biasa (KLB di Indonesia. Salah satu penyebab terjadinya KLB tersebut diduga sebagaiakibat perbedaan antigenesitas antara strain vaksin yang digunakan dengan strain virus campak liar yang beredar di Indonesia. Penelitian ini bertujuan mendapatkan gambaran tentang karakteristik genetik gen Haemagglutinin virus campak liar yang ada di Indonesia. Spesimen yang digunakan sebanyak 27 isolat virus penyebab KLB dari 17 propinsi selama periode tahun 2003-2010. Isolat virus dilakukan pemeriksaan secara RT-PCR dan sekuensing dengan metode Sanger. Hasil sekuensing dianalisis dengan menggunakan perangkat lunak Bioedit 7.0 dan MEGA 4.0. Hasil penelitian didapatkan perbedaan 10 asam amino antara virus campak strain vaksin CAM-70 dan virus campak liar pada posisi D416N; K424T; V451M; N455T; V466I; I473T; F476L; Y481S atau Y481N; H495N; G505D. Kesimpulan penelitian ini adalah terdapat perbedaan karakteristik genetik antara virus campak liar di Indonesia berbeda dengan strain virus vaksin CAM-70.Kata kunci : Campak, Analisis Molekuler, Hemagglutinin, CD46AbstractMeasles is caused by virus belonging to the genus Morbilivirus and Family Paramyxoviridae. Measles is still a public health problem because outbreak of measles still found in Indonesia. Outbreak is suspected as a result of differences in antigenicity between vaccine strains used with wild-type measles virus strains circulating in Indonesia. This study aims to get genetic characteristics of wild-type measles virus haemagglutinin gene in Indonesia. The specimens were used 27 viral isolates from 17 provinces period 2003-2010. Viral isolates examined by RT-PCR and sequencing with Sanger method. Sequencing analysis were conducted using Bioedit 7.0 and MEGA 4.0 software. The results showed 10 amino acid differences

  11. Genética e hanseníase

    Directory of Open Access Journals (Sweden)

    Bernardo Beiguelman

    Full Text Available As diferentes linhas de pesquisa utilizadas para investigar a importância dos fatores hereditários humanos na determinação da resistência/suscetibilidade à infecção pelo Mycobacterium leprae foram discutidas no presente trabalho. Uma síntese dessas abordagens permitiu analisar os resultados das investigações sobre associação da hanseníase com polimorfismos genéticos, distribuição familial da hanseníase, prevalência da hanseníase e distância genética, concordância da hanseníase em gêmeos e estudos genéticos sobre a reação de Mitsuda.

  12. A novel role of human holliday junction resolvase GEN1 in the maintenance of centrosome integrity.

    Directory of Open Access Journals (Sweden)

    Min Gao

    Full Text Available The maintenance of genomic stability requires accurate genome replication, repair of DNA damage, and the precise segregation of chromosomes in mitosis. GEN1 possesses Holliday junction resolvase activity in vitro and presumably functions in homology driven repair of DNA double strand breaks. However, little is currently known about the cellular functions of human GEN1. In the present study we demonstrate that GEN1 is a novel centrosome associated protein and we characterize the various phenotypes associated with GEN1 deficiency. We identify an N-terminal centrosome localization signal in GEN1, which is required and sufficient for centrosome localization. We report that GEN1 depletion results in aberrant centrosome numbers associated with the formation of multiple spindle poles in mitosis, an increased number of cells with multi-nuclei, increased apoptosis and an elevated level of spontaneous DNA damage. We find homologous recombination severely impaired in GEN1 deficient cells, suggesting that GEN1 functions as a Holliday junction resolvase in vivo as well as in vitro. Complementation of GEN1 depleted cells with various GEN1 constructs revealed that centrosome association but not catalytic activity of GEN1 is required for preventing centrosome hyper-amplification, formation of multiple mitotic spindles, and multi-nucleation. Our findings provide novel insight into the biological functions of GEN1 by uncovering an important role of GEN1 in the regulation of centrosome integrity.

  13. La genética de las poblaciones centroamericanas

    Directory of Open Access Journals (Sweden)

    Barrantes, Ramiro

    2005-01-01

    Full Text Available Las poblaciones centroamericanas no han sido objeto de muchos estudios genéticos con la excepción de análisis esporádicos de la variación entre y dentro de los grupos amerindios y de origen africano ubicados en el área. No obstante, en los últimos 15 años se efectuaron investigaciones sistemáticas en este sentido incluyendo poblaciones mestizas, particularmente las de Costa Rica y Panamá. En los amerindios se efectuaron estudios detallados de su estructura genética y las relaciones filogenéticas entre poblaciones en un contexto regional y continental. De manera general estos grupos mantienen una baja diversidad genética. Por otra parte, estimativas de la mezcla génica en la población mestiza muestran diferencias dentro y entre los distintos países del área. Al Valle Central de Costa Rica se le atribuye la condición de aislado con una supuesta constitución genética homogénea favorable para los estudios de desequilibrio de ligamiento y asociaciones con algunas enfermedades hereditarias; sin embargo, los resultados obtenidos con relación el flujo génico direccional, la amalgama de etnias y otros componentes de su estructura como la variación temporal y espacial de la consanguinidad y la migración indican una mayor heterogeneidad. Se muestran los resultados y conclusiones obtenidas y se plantean las perspectivas y tendencias al mediano plazo que vinculan los enfoques y métodos de la epidemiología y la antropología genética y el estudio genético de poblaciones.

  14. Craniostenose em gêmeos: estudo genético

    Directory of Open Access Journals (Sweden)

    Walter Carlos Pereira

    1968-09-01

    Full Text Available É relatada a ocorrência de formas clínicas diversas de craniostenose em gêmeos de sexo diferente. A menina apresentava obliteração completa da sutura coronaria e dos dois terços anteriores da sutura sagital; no menino a sutura sagital era a única afetada. O estudo genético mostrou que a craniostenose independe de aberrações cromossômicas, indicando ser transmitida por gens recessivos raros de natureza autossômica.

  15. Biotecnologia aplicada ao melhoramento genético do cafeeiro

    OpenAIRE

    2011-01-01

    O melhoramento genético do cafeeiro mediante técnicas convencionais é trabalhoso e demorado. A biotecnologia oferece estratégias alternativas para auxiliar na multiplicação e no desenvolvimento de novas variedades com resistência a estresses bióticos e abióticos, melhor qualidade de bebida e maturação mais uniforme dos frutos. As técnicas de cultura de tecidos têm possibilitado a obtenção de grande número de plantas e a garantia da uniformidade genética do material. O emprego de marcadores mo...

  16. Introducing AstroGen: the Astronomy Genealogy Project

    Science.gov (United States)

    Tenn, Joseph S.

    2016-12-01

    The Astronomy Genealogy Project (AstroGen), a project of the Historical Astronomy Division of the American Astronomical Society (AAS), will soon appear on the AAS website. Ultimately, it will list the world's astronomers with their highest degrees, theses for those who wrote them, academic advisors (supervisors), universities, and links to the astronomers or their obituaries, their theses when online, and more. At present the AstroGen team is working on those who earned doctorates with astronomy-related theses. We show what can be learned already, with just ten countries essentially completed.

  17. Genética, saúde e sociedade

    OpenAIRE

    2013-01-01

    O documentário apresenta quatro projetos de pesquisa realizados pelo Instituto de GenéticaMédica Populacional (INAGEMP) em diferentes regiões do país.As atividades mencionadasrepresentam uma amostra do trabalho do INAGEMP, que se estruturou de modo a colocar agenética médica populacional na prática ao associar pesquisa e atenção em saúde parapopulações afetadas por doenças com forte componente genético em sua etiologia.

  18. Divergência genética em genótipos de girassol Genetic divergence in sunflower genotypes

    Directory of Open Access Journals (Sweden)

    Edson Perito Amorim

    2007-12-01

    Full Text Available Uma investigação sobre a diversidade genética entre 15 genótipos de girassol, por meio de 12 características agronômicas, foi implementada no Instituto Agronômico, Campinas, Brasil. Análises de variância univariada e multivariada revelaram diferenças entre os genótipos. A distância generalizada de Mahalanobis indicou um alto grau de divergência genética. Os genótipos foram agrupados em três grupos. As características início do florescimento, 50% do florescimento, número de folhas e altura da inserção do capítulo contribuíram com grande parte da divergência genética observada. Por meio desses resultados, é possível identificar materiais divergentes e com características agronômicas complementares para o desenvolvimento de novos cultivares superiores.An investigation about the genetical diversity among fifteen sunflower genotypes using twelve agronomical characteristics was implanted at the Agronomic Institute, Campinas Brazil. Univariate and multivariate analyses of variance revealed the presence of differences among the genotypes. The generalized distance of Mahalanobis indicated the presence of genetic diversity. The genotypes were grouped into tree clusters. Among the investigated characteristics, the beginning of flowering, 50% flowering, leaf number and head height of chapter insertion exhibited high contribution towards genetic divergence. Through these studies it is possible to identify divergent material with further agronomical features for the development of new superior sunflower cultivars.

  19. A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors.

    Energy Technology Data Exchange (ETDEWEB)

    Rizwan-uddin; Nick Karancevic; Stefano Markidis; Joel Dixon; Cheng Luo; Jared Reynolds

    2008-04-23

    many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important crterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals.

  20. Epidemiologia genética: epidemiologia, genética ou nenhuma das anteriores?

    Directory of Open Access Journals (Sweden)

    Aguinaldo Gonçalves

    1990-12-01

    Full Text Available No esforço de contribuir para melhor entendimento da identidade da Epidemiologia Genética, são revistas sua concepção, campo de atuação, métodos e técnicas pertinentes e algumas instâncias de aplicação. Entendendo-a como a área de interesse dos fatores genéticos das doenças e suas interações ambientais, apresenta-se seu campo de atuação como constituído por dois segmentos: um descritivo, que lida com conhecimento da distribuição de tais afecções em famílias e populações, seu impacto a nível do coletivo e sua vigilância epidemiológica, bem como o estudo de seus determinantes; o segundo, caracterizado pela intervenção, refere-se às respectivas medidas preventivas. Em que pese possível limitação pela não-consideração de todas as situações existentes, particular atenção é destinada à revisão de métodos e técnicas que possam ser convergentemente aplicados, a partir de procedimentos genéticos e epidemiológicos. Entre eles, destacam-se como estudos de casos tanto metodologias laboratoriais (como os dermatóglifos quanto quantitativos, como cálculo de herdabilidade e análise multivariada. Alguns objetos de estudo são tomados como instância de aplicação, por contarem com investigações específicas em nosso meio: a hanseníase, o hidrargirismo e a esquizofrenia.In an attempt to contribute to a better undestanding of the identity of Genetic Epidemiology, we review its conception, its field of influence, its appropriate methods and techniques and, at last, some of its applications. Genetic Epidemiology involves the study of genetic factors acting on diseases and on their environmental interactions. These includes two major areas: a descriptive one, related to the distribution of such conditions in families and populations, to the epidemiologic surveillance and to the study of determinants; and another characterized by intervention, which is related to preventive measures. Because of the dificulty in

  1. A Novel Role of Human Holliday Junction Resolvase GEN1 in the Maintenance of Centrosome Integrity

    DEFF Research Database (Denmark)

    Gao, M.; Danielsen, Jannie Michaela Rendtlew; Wei, L.-Z.;

    2012-01-01

    but not catalytic activity of GEN1 is required for preventing centrosome hyper-amplification, formation of multiple mitotic spindles, and multi-nucleation. Our findings provide novel insight into the biological functions of GEN1 by uncovering an important role of GEN1 in the regulation of centrosome integrity...

  2. 78 FR 28940 - Ninth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-05-16

    ... Federal Aviation Administration Ninth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held June 4, 2013 from...

  3. 78 FR 5860 - Eighth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-01-28

    ... Federal Aviation Administration Eighth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... meeting of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held February 7,...

  4. 78 FR 54509 - Tenth Meeting: RTCA Next Gen Advisory Committee (NAC)

    Science.gov (United States)

    2013-09-04

    ... Federal Aviation Administration Tenth Meeting: RTCA Next Gen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), U.S. Department of Transportation (DOT). ACTION: Notice of RTCA NextGen... of the RTCA NextGen Advisory Committee (NAC). DATES: The meeting will be held September 19, 2013...

  5. Mapeo genético en camarón blanco litopenaeus vannamei

    OpenAIRE

    Erazo Maldonado, Edna Constanza

    2002-01-01

    Mapeo genético en camarón blanco Litopenaeus vannamei Se presenta el primer mapa genético de Litopenaeus vannamei basado en la técnica de AFLPs a partir de una familia proveniente de un cruce comercial y con la información genética de un solo progenitor y 42 hijos.

  6. Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates.

    Science.gov (United States)

    Bellendir, Stephanie P; Rognstad, Danielle J; Morris, Lydia P; Zapotoczny, Grzegorz; Walton, William G; Redinbo, Matthew R; Ramsden, Dale A; Sekelsky, Jeff; Erie, Dorothy A

    2017-05-19

    Human GEN1 and yeast Yen1 are endonucleases with the ability to cleave Holliday junctions (HJs), which are proposed intermediates in recombination. In vivo, GEN1 and Yen1 function secondarily to Mus81, which has weak activity on intact HJs. We show that the genetic relationship is reversed in Drosophila, with Gen mutants having more severe defects than mus81 mutants. In vitro, DmGen, like HsGEN1, efficiently cleaves HJs, 5΄ flaps, splayed arms, and replication fork structures. We find that the cleavage rates for 5΄ flaps are significantly higher than those for HJs for both DmGen and HsGEN1, even in vast excess of enzyme over substrate. Kinetic studies suggest that the difference in cleavage rates results from a slow, rate-limiting conformational change prior to HJ cleavage: formation of a productive dimer on the HJ. Despite the stark difference in vivo that Drosophila uses Gen over Mus81 and humans use MUS81 over GEN1, we find the in vitro activities of DmGen and HsGEN1 to be strikingly similar. These findings suggest that simpler branched structures may be more important substrates for Gen orthologs in vivo, and highlight the utility of using the Drosophila model system to further understand these enzymes. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  7. GenBank blastx search result: AK058467 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 1p34.2-36.11 Contains the 3' end of the KPNA6 gene for karyopherin alpha 6 (importin alpha 7), a novel g...ene (DKFZp451J0118), a novel gene (MGC1203) a novel gene, a novel gene (FLJ10547), a novel gene, a novel gen

  8. Justicia y genética: compensando las diferencias

    Directory of Open Access Journals (Sweden)

    Alejandra Zúñiga-Fajuri

    2013-01-01

    Full Text Available Se analizan los dilemas morales asociados a los avances científicos que en la actualidad nos exigen repensar el concepto de igualdad equitativa de oportunidades. Asimismo, se pasa revista a la discusión filosófica en torno al origen de las desventajas sociales y genéticas que permiten las desigualdades sociales.

  9. Safer Systems: A NextGen Aviation Safety Strategic Goal

    Science.gov (United States)

    Darr, Stephen T.; Ricks, Wendell R.; Lemos, Katherine A.

    2008-01-01

    The Joint Planning and Development Office (JPDO), is charged by Congress with developing the concepts and plans for the Next Generation Air Transportation System (NextGen). The National Aviation Safety Strategic Plan (NASSP), developed by the Safety Working Group of the JPDO, focuses on establishing the goals, objectives, and strategies needed to realize the safety objectives of the NextGen Integrated Plan. The three goal areas of the NASSP are Safer Practices, Safer Systems, and Safer Worldwide. Safer Practices emphasizes an integrated, systematic approach to safety risk management through implementation of formalized Safety Management Systems (SMS) that incorporate safety data analysis processes, and the enhancement of methods for ensuring safety is an inherent characteristic of NextGen. Safer Systems emphasizes implementation of safety-enhancing technologies, which will improve safety for human-centered interfaces and enhance the safety of airborne and ground-based systems. Safer Worldwide encourages coordinating the adoption of the safer practices and safer systems technologies, policies and procedures worldwide, such that the maximum level of safety is achieved across air transportation system boundaries. This paper introduces the NASSP and its development, and focuses on the Safer Systems elements of the NASSP, which incorporates three objectives for NextGen systems: 1) provide risk reducing system interfaces, 2) provide safety enhancements for airborne systems, and 3) provide safety enhancements for ground-based systems. The goal of this paper is to expose avionics and air traffic management system developers to NASSP objectives and Safer Systems strategies.

  10. Distributed Generation Market Demand Model (dGen): Documentation

    Energy Technology Data Exchange (ETDEWEB)

    Sigrin, Benjamin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Gleason, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States); Preus, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States); Baring-Gould, Ian [National Renewable Energy Lab. (NREL), Golden, CO (United States); Margolis, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-02-01

    The Distributed Generation Market Demand model (dGen) is a geospatially rich, bottom-up, market-penetration model that simulates the potential adoption of distributed energy resources (DERs) for residential, commercial, and industrial entities in the continental United States through 2050. The National Renewable Energy Laboratory (NREL) developed dGen to analyze the key factors that will affect future market demand for distributed solar, wind, storage, and other DER technologies in the United States. The new model builds off, extends, and replaces NREL's SolarDS model (Denholm et al. 2009a), which simulates the market penetration of distributed PV only. Unlike the SolarDS model, dGen can model various DER technologies under one platform--it currently can simulate the adoption of distributed solar (the dSolar module) and distributed wind (the dWind module) and link with the ReEDS capacity expansion model (Appendix C). The underlying algorithms and datasets in dGen, which improve the representation of customer decision making as well as the spatial resolution of analyses (Figure ES-1), also are improvements over SolarDS.

  11. GenOVa: a computer program to generate orientational variants

    OpenAIRE

    Cayron, Cyril

    2007-01-01

    A computer program called GenOVa, written in Python, calculates the orientational variants, the operators (special types of misorientations between variants) and the composition table associated with a groupoid structure. The variants can be represented by three-dimensional shapes or by pole figures.

  12. A New Parent Generation: Meet Mr. and Mrs. Gen X

    Science.gov (United States)

    Howe, Neil

    2010-01-01

    Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. Gen-X parents and Boomer parents belong to two neighboring generations, each possessing its own location in history and its own peer personality. They are similar in some respects, but clearly different in…

  13. Meet Mr. and Mrs. Gen X: A New Parent Generation

    Science.gov (United States)

    Howe, Neil

    2010-01-01

    Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. In the early 1990s, Gen Xers began joining parent-teacher associations in the nation's elementary schools. Around 2005, they became the majority of middle school parents. By the fall of 2008, they took over…

  14. GenBank blastx search result: AK241821 [KOME

    Lifescience Database Archive (English)

    Full Text Available e for comparative gene identification transcript 94 (CGI-94), complete sequence. PRI 5e-40 1 ... ...3A3 gene for 60kDa splicing factor 3a subunit 3, the FHL3 gene for four and a half LIM domains 3 and the gen

  15. GenBank blastx search result: AK241192 [KOME

    Lifescience Database Archive (English)

    Full Text Available e for comparative gene identification transcript 94 (CGI-94), complete sequence. PRI 1e-114 1 ... ...3A3 gene for 60kDa splicing factor 3a subunit 3, the FHL3 gene for four and a half LIM domains 3 and the gen

  16. Boomers, Gen-Xers, and Millennials: Understanding the "New Students."

    Science.gov (United States)

    Oblinger, Diana

    2003-01-01

    Describes characteristics of the "new" college student, who may be an adult learner from the Baby Boomer era, a high school member of the "Millennial" generation, or a "Gen-Xer." Explores the learning styles of each type of student and discusses the importance of technology to each group. (SLD)

  17. GenBank blastn search result: AK064582 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK064582 002-112-F03 AY007820.1 Daucus carota ATPase8 (ATP8) gene, ATP8-Sp1b allele, complete cds; chimeric... ATPase9 (ATP9) gene, ATP9-Sp3 allele, complete cds; and chimeric ATPase6 (ATP6) gen

  18. Le CERN, chance ou risque pour Genève

    CERN Document Server

    Maiani, Luciano; Wenninger, Horst; CERN. Geneva

    1998-01-01

    Le CERN, Chance ou Risque pour Gen?ve? D?bat public contradictoire organis? avec le soutien de la Tribune de Gen?ve Lieu: Salle II, CICG,15 Rue de Varemb?. Date: Lundi 19 octobre 1998, 20h00 D?roulement de la soir?e: 1. Ouverture M. Ren? KÏchlin, Pr?sident du Grand Conseil M. Luciano Maiani, Directeur g?n?ral d?sign? du CERN 2. Introduction: Un regard critique sur le CERN (? d?signer) Le projet LHC (Manfred Buhler Broglin CERN) 3. D?bat th?matique men? par le mod?rateur (Marco Cattaneo, r?dacteur en chef de la Tribune de Gen?ve) entre trois representants de lÕopinion publique (Ren? Longet, d?put? socialiste; Yves de Pr?ville, physicien; Jacques Mirenowicz, chercheur) et trois repr?sentants du Cern (Horst Wenninger, Directeur de la Recherche/Technique, CERN; Manfred Buhler Broglin, Administra-teur du projet LHC, CERN; Maurice Bourquin, Professeur de Physique ? l'Universit? de Gen?ve et D?l?gu? de la Suisse au Conseil du CERN). Les th?mes abord?s seront: Ð A quoi sert le CERN? Ð Quel est lÕimpact ?conomiqu...

  19. GenSVM: a generalized multiclass support vector machine

    NARCIS (Netherlands)

    G.J.J. van den Burg (Gerrit); P.J.F. Groenen (Patrick)

    2016-01-01

    textabstractTraditional extensions of the binary support vector machine (SVM) to multiclass problems are either heuristics or require solving a large dual optimization problem. Here, a generalized multiclass SVM is proposed called GenSVM. In this method classification boundaries for a K-class proble

  20. Carinichremylus gen. nov. (Hymenoptera: Braconidae: Pambolinae) from Peru

    NARCIS (Netherlands)

    Achterberg, van C.

    1999-01-01

    A new genus of the subfamily Pambolinae (Braconidae) is reported from Peru (Carinichremylus gen. nov.; type species: Carinichremylus peleopodae spec. nov.), illustrated and described. The type species has been reared from a Peleopoda spec. (Lepidoptera: Oecophoridae). The new genus is closely

  1. Genetic Structure and Diversity of Four Chilean Sheep Breeds Estructura Genética y Diversidad de Cuatro Razas Ovinas Chilenas

    Directory of Open Access Journals (Sweden)

    Rodrigo de la Barra

    2010-12-01

    Full Text Available Chilean sheep breeds have a significant founder effect since they descend from very few parents, in some cases population inbreeding has increased and in others there have been significant differentiation processes as compared to their original population. The objective of this study was to estimate the current status of genetic diversity in sheep herds, which comprise the main sheep breeds in Chile, using molecular methods. Animals from four breeding herds were sampled and analyzed. The breeds studied were Corriedale, Suffolk Down, Romney Marsh and Chilota, these are the predominant numerical pure breeds in Chile. From each breed population 40 animals were sampled. Genetic characterization was done using nine microsatellite molecular markers (SSR recommended by FAO-ISAG. Results showed that in the Chilean sheep herds there are low genetic complementarities among breeds and a high risk of losing genetic diversity due to inbreeding in Chilota and Romney Marsh breeds.Las razas ovinas presentes en Chile presentan un importante efecto fundador, al descender de un reducido número de progenitores. En algunos casos se sospecha que ha aumentado el grado de consanguinidad poblacional, mientras que en otros se observan procesos significativos de diferenciación respecto a sus poblaciones de origen. El objetivo del presente estudio fue estimar el estado actual de la diversidad genética de los planteles que gestionan las principales razas utilizadas en la ganadería ovina chilena. Para ello se utilizaron las razas Corriedale, Sufolk Down, Romney Marsh y Chilota, dado que son las predominantes en Chile como poblaciones puras. Se analizaron 40 animales de cada raza, pertenecientes a cuatro planteles de reproductores. Para la caracterización genética de los animales se utilizaron nueve marcadores moleculares de tipo microsatélite (SSR recomendados por FAO-ISAG. Los resultados indican una baja complementariedad genética entre las razas ovinas

  2. CARD15 in inflammatory bowel disease and Crohn's disease phenotypes: an association study and pooled analysis.

    NARCIS (Netherlands)

    Oostenbrug, L.E.; Nolte, I.M.; Oosterom, E.; Steege, G. van der; Meerman, G.J. te; Dullemen, H.M. van; Drenth, J.P.H.; Jong, D.J. de; Linde, K. van der; Jansen, P.L.M.; Kleibeuker, J.H.

    2006-01-01

    BACKGROUND: Three major polymorphisms of the Caspase-Activation Recruitment Domain containing protein 15 gene have been described to be associated with Crohn's disease. Genotype-phenotype studies reported in literature provide conflicting data on disease localisation and behaviour. We investigated

  3. NOD2/CARD15 genotype and common gastrointestinal diseases in 43 600 individuals

    DEFF Research Database (Denmark)

    Yazdanyar, S.; Nordestgaard, B.G.

    2010-01-01

    associate with risk of nine common gastrointestinal diseases. Design and setting. We genotyped 43 596 white individuals from the Danish general population followed for 31 years, during which time 782 developed oesophagitis and reflux, 1395 ulcus ventriculi and duodeni, 1384 gastritis and dyspepsia, 1407...

  4. NOD2/CARD15 genotype and common gastrointestinal diseases in 43 600 individuals

    DEFF Research Database (Denmark)

    Yazdanyar, S.; Nordestgaard, B.G.

    2010-01-01

    appendicitis, 646 irritable bowel syndrome, 1301 infectious diseases of the gastrointestinal tract, 681 anal fissure, fistula and abscess, 826 gastrointestinal cancer and 161 developed cancer in liver and pancreas. Results. Some 89% were non-carriers, 11% heterozygotes, 0.15% homozygotes and 0.23% compound...... heterozygotes. Cumulative incidences differed by genotype for appendicitis (log-rank P = 0.02), anal fissure, fistula and abscess (P = 0.003) and gastrointestinal cancer (P = 0.004), but not for any of the other endpoints. Compared with non-carriers, age and sex adjusted hazard ratios were 2.7 (95% CI 1.......4-5.5) for appendicitis amongst compound heterozygotes, 3.2 (1.3-7.8) for anal fissure, fistula and abscess amongst compound heterozygotes, and 3.8 (1.6-9.2) for gastrointestinal cancer amongst homozygotes, whilst other genotypes did not have increased risk. The increased risk of gastrointestinal cancer amongst...

  5. Efectos de la gestión selvícola pasada y presente sobre la diversidad genética actual y futura de Quercus pyrenaica Willd. en Sierra Nevada

    Directory of Open Access Journals (Sweden)

    M. Valbuena Carabaña

    2014-01-01

    Full Text Available Quercus pyrenaica Willd. es un roble mediterráneo-occidental, ampliamente distribuido en la península ibérica que presenta una extraordinaria capacidad de rebrote, especialmente de raíz. Tradicionalmente ha sido aprovechado para leñas en monte bajo y, menos frecuentemente, adehesado para el uso ganadero. El abandono de la gestión tradicional mayoritaria ha puesto de manifiesto el estado de degradación de sus masas (falta decrecimiento, puntisecado, ausencia de fructificación, que de forma teórica se había atribuido al agotamiento de las cepas y a la falta de diversidadgenética. Sin embargo, el análisis reciente mediante marcadores moleculares microsatélites de numerosos rebollares, incluidos los que aquí se presentan, permite descartar la supuesta falta de variabilidad genética. No obstante, la falta de concordancia entre la estructuras forestales de montes bajos y adehesados y el origen asexual o sexual de sus pies, junto a la heterogeneidad en el tamaño y composición de las cepas dificulta la interpretación de la estructura clonal actual en cada monte. Este trabajo, en el que se evalúa el estado de conservación de los recursos genéticos de Q.pyrenaica en uno de los territorios más intensamente aprovechados por el hombre a lo largo de la historia, ilustra la importante resiliencia que presenta la especie frente al manejo tradicional en monte bajo. Mediante el estudio de tres rodales localizados en el mismo robledal en el Parque Nacional de Sierra Nevada, se analiza el efecto de la gestión pasada (en monte bajo y adehesado y presente (en un rodal de monte bajo resalveado sobre la diversidad genética y la estructura clonal de la especie. Además, se evalúa la evolución futura de la diversidad genética a través del análisis del regenerado en los tres rodales en función del manejo selvícola que han experimentado. A pesar de que los montes bajos presentan mayores niveles de diversidad genética para la cohorte adulta

  6. Delineamento de experimentos em genética genômica Experimental design in genetical genomics

    Directory of Open Access Journals (Sweden)

    Guilherme Jordão de Magalhães Rosa

    2007-07-01

    Full Text Available Genética genômica é um termo utilizado para representar o estudo de processos genéticos controladores de caracteres fenotípicos de herança complexa, a partir da análise conjunta de informação relativa a fenótipos, estruturas de parentesco, marcadores moleculares e expressão gênica. Estudos de genética genômica são utilizados, por exemplo, para a estimação da herdabilidade de níveis de transcrição, para o mapeamento de locos controladores da expressao gênica (eQTL, do inglês expression Quantitative Trait Loci, e para o estudo de redes regulatórias. Genética genômica geralmente envolve experimentos com microarrays, os quais são ainda bastante caros e trabalhosos, limitando o tamanho amostral e conseqüentemente o poder estatístico de tais estudos. Desta maneira, é essencial que tais experimentos sejam otimizados do ponto de vista do delineamento, a partir de criteriosa escolha das amostras (indivíduos a serem utilizadas, e do controle rigoroso dos vários fatores que podem afetar as variáveis-resposta de interesse. Outro ponto fundamental na condução de tais experimentos refere-se à marcação das amostras de mRNA com os fluoróforos e ao pareamento das mesmas em cada lâmina de microarray, os quais devem ser cuidadosamente planejados para que não haja confundimento entre estes efeitos e os fatores biológicos de interesse. Nesta apresentação serão discutidas algumas estratégias para o planejamento de estudos de genética genômica, incluindo a seleção de indivíduos objetivando-se a maximização da dissimilaridade genética ou do número de eventos de recombinação, bem como a condução eficiente dos ensaios com microarrays para diferentes objetivos experimentais.Genetical genomics experiments combine information on phenotypic traits, molecular markers and gene expression to study the genetic mechanisms governing variation in complex traits. Such studies can be used, for example, to estimate

  7. DOE/NNSA perspective safeguard by design: GEN III/III+ light water reactors and beyond

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Paul Y [Los Alamos National Laboratory

    2010-12-10

    An overview of key issues relevant to safeguards by design (SBD) for GEN III/IV nuclear reactors is provided. Lessons learned from construction of typical GEN III+ water reactors with respect to SBD are highlighted. Details of SBD for safeguards guidance development for GEN III/III+ light water reactors are developed and reported. This paper also identifies technical challenges to extend SBD including proliferation resistance methodologies to other GEN III/III+ reactors (except HWRs) and GEN IV reactors because of their immaturity in designs.

  8. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus

    Directory of Open Access Journals (Sweden)

    Raden Roro Sri Pudji Sinarni Dewi

    2016-04-01

    menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein. Pengamatan gen EGFP (enhanced green fluorescent protein pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus ditujukan untuk mendapatkan informasi mengenai kemampuan promoter -aktin ikan mas dalam mengendalikan ekspresi gen EGFP. Gen EGFP diintroduksikan ke dalam sperma ikan patin siam menggunakan metode elektroporasi. Sperma yang telah dielektroporasi digunakan untuk membuahi sel telur ikan patin siam. Pengamatan ekspresi gen EGFP dilakukan setiap enam jam dimulai dari embrio fase 2 sel sampai larva. Berdasarkan hasil penelitian, gen EGFP terekspresi pada fase embrio dan larva ikan patin siam. Puncak ekspresi gen EGFP terjadi pada fase neurula dan menurun pada fase larva. Berdasarkan penelitian ini maka ikan patin siam transgenik telah berhasil dibentuk dan promoter -aktin ikan mas terbukti aktif dalam mengarahkan ekspresi gen asing (GFP di dalam tubuh ikan patin siam.

  9. Justicia en salud y genética

    Directory of Open Access Journals (Sweden)

    Maria Graciela De Ortuzar

    2014-06-01

    Full Text Available Las expectativas puestas en el conocimiento genético exceden el ámbito de la medicina tradiciona, debido a que la intervención directa en la lotería natural demandaría el replanteamiento de conceptos centrales de justicia en salud: necesidades médicas, enfermedad, normalidad, e igualdad de oportunidades en el acceso a la salud. El punto en debate es sí el replanteo de dichos conceptos conlleva un cambio radical en las teorías de justicia (libertariana y/o liberal, mostrando su obsolescencia, o sí simplemente se requiere ampliar dichos conceptos claves por fallas estructurales en las mismas teorías. Como hipótesis general considero que los supuestos cuestionamientos, lejos de socavar las bases de las teorías de justicia, sólo ponen en evidencia sus viejos problemas estructurales. Por razones expositivas, dividiré la presentación tres partes. En la Primera parte, analizo la teoría libertariana, estudiando las contradicciones del modelo a través del impacto de la información genética en el seguro privado de salud. En la Segunda Parte, desarrollo la propuesta alternativa liberal rawlsianadanielsiana del modelo de seguro público, evaluando las implicaciones de la genética a partir de la crítica de su concepto biológico de enfermedad y su restricción al acceso a la salud por necesidades naturales. En la Tercera parte presento un modelo integral de necesidades y capacidades básicas, comprendiendo la prevención, el tratamiento y el mejoramiento moralmente permisible (genético y no genético.Mi aporte principal consiste en la elaboración de este modelo normativo integral de necesidades y capacidades para la regulación conjunta de la información y terapia genética con los restantes problemas de salud.

  10. Mecanismos genéticos en la predisposición hereditaria al cáncer colorrectal Genetic mechanisms in the hereditary predisposition to colorectal cancer

    Directory of Open Access Journals (Sweden)

    A. Alonso

    2006-04-01

    Full Text Available Una proporción de los cánceres colorrectales presentan algún tipo de predisposición genética que es posible reconocer en la práctica clínica. Desde los clásicos patrones hereditarios dominantes de la poliposis adenomatosa familiar o el cáncer de colon hereditario no asociado a poliposis, pasando por la transmisión recesiva mostrada por la poliposis asociada al gen MYH, hasta llegar a los novedosos síndromes de la "vía serrada" o los alelos de baja penetrancia, el descubrimiento de nuevos genes y el mejor conocimiento de los mecanismos de acción de los ya conocidos, están permitiendo comprender nuevos aspectos de la carcinogénesis colorrectal que arrojan nueva luz sobre algunas de las observaciones de patrones de agregación familiar al cáncer de colon que permanecían inexplicadas.A proportion of colorectal cancers shows some type of genetic predisposition that can be recognised in clinical practice. From the classical dominant inheritance pattern of familial adenomatous polyposis or hereditary non-polyposis colorectal cancer, through the recessive transmission of the MYH associated polyposis, to the new syndromes of the "serrated pathway" or low-penetrance alleles, the discovery of new genes and a deeper understanding of the mechanisms of action of already-known ones are enabling us to understand new aspects of the colorectal carcinogenesis. This is throwing a new light on some of the observed familial aggregation patterns which had remained unexplained.

  11. New species of Monepidosis Mamaev, 1966 and Antipodosis gen. nov., a closely related genus from New Zealand (Diptera, Cecidomyiidae

    Directory of Open Access Journals (Sweden)

    Mathias Jaschhof

    2016-04-01

    Full Text Available Three new species of Monepidosis Mamaev, 1966, a Holarctic genus of Porricondylinae (Diptera, Cecidomyiidae, are described: M. heterocera sp. nov. from Sweden and Germany, M. scepteroides sp. nov. from Sweden, and M. shikokuensis sp. nov. from Japan. A new porricondyline genus, Antipodosis gen. nov., is introduced for eight new species from New Zealand, named A. australis gen. et sp. nov., A. elongata gen. et sp. nov., A. granvillensis gen. et sp. nov., A. pureora gen. et sp. nov., A. rakiura gen. et sp. nov., A. rotoiti gen. et sp. nov., A. rotoroa gen. et sp. nov., and A. waipapa gen. et sp. nov. Male genitalic morphology indicates that Monepidosis and Antipodosis gen. nov. are closely related, together forming the Monepidosis group of genera, which stands out from the other Porricondylini. Monepidosis spatulata Spungis, 2006, a species originally described from Latvia and Lithuania, is for the first time reported to occur in Sweden.

  12. Enfermedades de base genética Genetically based diseases

    Directory of Open Access Journals (Sweden)

    D. González-Lamuño

    2008-01-01

    Full Text Available La genética constituye uno de los mayores avances científicos del siglo XX, que comienza con el redescubrimiento de las leyes de Mendel y termina con la elaboración del primer "borrador" de la secuencia completa del genoma humano. La genética utiliza diferentes estrategias de investigación, como los estudios de gemelos y de adopción, que investigan la influencia de los factores genéticos y ambientales, y las estrategias para identificar genes específicos (genética molecular. Además del importante grado de discapacidad que generan, el impacto social de las enfermedades hereditarias es enorme, por su carácter potencialmente recurrente en una misma familia y por el elevado coste socio-sanitario derivado de la enorme carga de cuidados que requiere. El diagnóstico de las enfermedades hereditarias presenta características diferenciadoras muy significativas ya que el resultado de un diagnóstico genético tiene no sólo efectos sobre el paciente sino también sobre todos los individuos emparentados. Por tanto, la unidad de estudio en el diagnóstico genético es la familia y todo proceso de diagnóstico implica una investigación familiar. También conviene tener en cuenta que los protocolos de diagnóstico se desarrollan de forma paralela a la investigación básica y generalmente están poco estandarizados. Los resultados obtenidos en los estudios genéticos y el tipo de información que se facilita al paciente y a su familia deben ser matizados dentro del proceso del "consejo genético".Genetics is one of the greatest scientific advances of the XX century, which begins with the rediscovery of Mendel’s laws and culminates in the elaboration of the first "draft" of the complete sequence of the human genome. Genetics employs different research strategies, such as the study of twins and adoption, investigating the influence of genetic and environmental factors, and strategies for identifying specific genes (molecular genetics. Besides the

  13. GENLAB, Laboratorio Virtual de Genética

    Directory of Open Access Journals (Sweden)

    García S.

    2001-06-01

    Full Text Available GenLab es el nombre que tiene el software diseñado por nosotros en el cual se modela el pro-ceso meiótico y la fecundación en organismos diploides. El objetivo de esta aplicación es el deilustrar el cruce de un resultado determinado, tratando de ser lo más ajustados a la realidad.La modelación de la reproducción sexual se realiza internamente y el GenLab se limita a presen-tar los resultados según el número de la descendencia seleccionado, para un cruce específico,esto significa que se puede escoger una gran cantidad de características para los parentalesy se puede estudiar la frecuencia de estos en la descendencia.

  14. Gen y ética. La inocencia perdida

    OpenAIRE

    Orozco-Martínez, Carlos E.

    1996-01-01

    Después del lanzamiento de las bombas atómicas en agosto de 1945 en Hiroshima y Nagasaki, la ética en la ciencia se convirtió en un tema de interés público con complejas implicaciones sociales. El artículo reflexiona sobre la ética en la investigación genética, vista desde la posibilidad de mejorar la salud de los humanos al predecir o evitar enfermedades, pero también como una puerta hacia la manipulación genética, la discriminación de los débiles y los enfermos, e incluso la alteración del ...

  15. Estructura y diversidad genética en vacas Holstein de Antioquia usando un polimorfismo del gen bGH

    Directory of Open Access Journals (Sweden)

    Juan Rincon F.

    2013-03-01

    Full Text Available Objetivo. Determinar las frecuencias alélicas y genotípicas del polimorfismo del intrón 3 del gen bGH y estimar algunos parámetros de estructura poblacional en ganado Holstein. Materiales y métodos. El estudio se realizó con 1366 vacas Holstein en 120 hatos de 11 municipios del departamento de Antioquia. Se extrajo DNA por el método de Salting out y la genotipificación se realizó usando la técnica de PCR-RFLPs. La diversidad genética se determinó mediante la comparación de las heterocigosidades, El equilibrio de Hardy-Weinberg (HW y la diferenciación genética entre las poblaciones se realizó usando el software Arlequín 2.0 Las frecuencias alélicas y genotípicas se evaluaron mediante el paquete estadístico SAS®. Resultados. Las frecuencias genotípicas encontradas fueron 0.764 (+/+, 0.223 (+/- y 0.013 (-/- y las frecuencias alélicas 0.876 (+ y 0.124 (-. No se encontraron desviaciones del Equilibrio de Hardy Weinberg en ninguna de las subpoblaciones. La diversidad genética determinada mediante la comparación de las heterocigosidades fue relativamente baja entre poblaciones pero al interior de estas no. El valor de FST de toda la población fue de 0.0068 y significativo (p<0.05, algunos FST pareados también lo fueron, tomando valores desde 0.0 a 0.13. Los estadísticos FIT y FIS no fueron significativos. Conclusiones. El gen bGH es un candidato interesante para evaluar características de importancia económica ya que no parece haber sido sometido a selección directa, presenta una variabilidad media en las poblaciones, observándose diferenciación genética significativa entre distintos municipios, producto de los diferentes sistemas de producción y acceso a las biotecnologías.

  16. Dissimilaridade genética entre genótipos de aveia Genetic dissimilarity among oat genotypes

    Directory of Open Access Journals (Sweden)

    Volmir Sergio Marchioro

    2003-04-01

    Full Text Available Dezoito genótipos de aveia foram testados quanto à dissimilaridade genética, com e sem o controle de moléstias da parte aérea. As variáveis avaliadas foram rendimento de grãos desaristados, peso de mil grãos, peso do hectolitro, estatura de planta e dias da emergência à floração. Foram empregados análises por variáveis canônicas e técnicas de agrupamento por meio dos métodos de otimização de Tocher e o método hierárquico do vizinho mais próximo, tendo como medida de dissimilaridade a distância generalizada de Mahalanobis. Pelos resultados, constatou-se significativa dissimilaridade genética entre os genótipos, indicando a existência de variabilidade para os caracteres avaliados. O método de Tocher e o método do vizinho mais próximo permitiram a separação dos genótipos em grupos distintos, possibilitando a identificação de futuros genitores que possam ser utilizados em cruzamentos artificiais que produzam progênies com maior heterose. Os caracteres que mais contribuíram para a dissimilaridade genética foram o peso do hectolitro e a estatura de planta.Eighteen oat genotypes were tested for genetic dissimilarity, with and without shoot disease control. The evaluated variables were grain yield, weight of one thousand grains, test weight, plant stature and days from emergence to flowering. Cannonical variables and clustering methods by Tocher's optimization test and the hierarchical method of nearest neighbor were employed, having as a measure of dissimilarity the general distance of Mahalanobis. The results showed a significant genetic dissimilarity among the genotypes, indicating the presence of genetic variability for the evaluated traits. Tocher's and nearest neighbor methods allowed the separation of genotypes in distinct groups, enabling the identification of potential parents for artificial crosses to obtain progenies with higher heterosis. The major traits contributing to genetic dissimilarity in our study

  17. NextGen Avionics Roadmap, Version 1.2

    Science.gov (United States)

    2010-09-21

    operators at remote locations. Cost and Benefit Considerations Costs to an aircraft operator, whether airline or military come in two forms -- capital... sense of the numbers and types of enablers that may be necessary to support operations that will be in- tegral to NextGen. Historical lead-in times...prediction information. Global Navigation Satellite System ( GNSS ), ADS-B Out, Aircraft Characteristic Database, Aircraft Wake Database, Wake

  18. Applications of Dredging and Beach Fills in GenCade

    Science.gov (United States)

    2016-06-01

    based on the methodology employed in the Inlet Reservoir Model ( IRM ), which assumes that if a jetty is not present at an inlet, all of the sand...system is at equilibrium. Further details about the IRM formulation within GenCade can be found in Frey et al. (2012, 2014). Figure 13 shows the...apportionment of transported sediment it received in the IRM . The only alternative where ERDC/CHL CHETN-IV-109 June 2016 10 the shoal does not

  19. Variabilidad genética en Prosopis ferox (Mimosaceae

    Directory of Open Access Journals (Sweden)

    Alicia D. Burghardt

    2004-01-01

    Full Text Available Prosopis ferox (Mimosaceae es una especie arbustiva o arbórea espinosa que se distribuye desde el Sur de Bolivia hasta el noroeste de la Argentina. En la provincia de Jujuy se encuentra a grandes alturas (entre los 2400 y los 3700 m s.m.. Existe una gran variabilidad morfológica, especialmente en cuanto a las dimensiones del fruto y la cantidad de semillas por fruto, ambas características importantes debido al uso de esta planta como forraje. Con el objeto de verificar si existe además variabilidad genética, se realizó un estudio electroforético de proteínas seminales de árboles procedentes de distintas localidades de la provincia de Jujuy. Los patrones polipeptídicos obtenidos por SDS-PAGE presentaron en total 26 bandas. Cada población se caracterizó por sus patrones de presencia-ausencia de bandas, habiéndose encontrado variabilidad intrapoblacional (polimorfismo en algunas de ellas, siendo otras genéticamente homogéneas. Los índices polimórficos en poblaciones de P. ferox son comparables a los obtenidos previamente en P. ruscifolia. La variabilidad genética interpoblacional hallada por medio del estudio electroforético de las proteínas seminales hace suponer la existencia de ecotipos

  20. The GenABEL Project for statistical genomics.

    Science.gov (United States)

    Karssen, Lennart C; van Duijn, Cornelia M; Aulchenko, Yurii S

    2016-01-01

    Development of free/libre open source software is usually done by a community of people with an interest in the tool. For scientific software, however, this is less often the case. Most scientific software is written by only a few authors, often a student working on a thesis. Once the paper describing the tool has been published, the tool is no longer developed further and is left to its own device. Here we describe the broad, multidisciplinary community we formed around a set of tools for statistical genomics. The GenABEL project for statistical omics actively promotes open interdisciplinary development of statistical methodology and its implementation in efficient and user-friendly software under an open source licence. The software tools developed withing the project collectively make up the GenABEL suite, which currently consists of eleven tools. The open framework of the project actively encourages involvement of the community in all stages, from formulation of methodological ideas to application of software to specific data sets. A web forum is used to channel user questions and discussions, further promoting the use of the GenABEL suite. Developer discussions take place on a dedicated mailing list, and development is further supported by robust development practices including use of public version control, code review and continuous integration. Use of this open science model attracts contributions from users and developers outside the "core team", facilitating agile statistical omics methodology development and fast dissemination.

  1. Crescimento de genótipos de frangos tipo caipira

    Directory of Open Access Journals (Sweden)

    R. C. Veloso

    2015-10-01

    Full Text Available RESUMOObjetivou-se com este trabalho comparar o padrão de crescimento, mediante ajustes das respectivas curvas de crescimento por modelos não lineares, bem como estudar o desenvolvimento de cortes de carcaça em relação ao peso da carcaça em diferentes genótipos de frangos tipo caipira. Foram utilizados 840 pintos de um dia, machos, distribuídos em delineamento inteiramente ao acaso, dos seguintes genótipos da linhagem Redbro: Caboclo, Carijó, Colorpak, Gigante Negro, Pesadão Vermelho, Pescoço Pelado e Tricolor. As aves foram alojadas em 28 boxes, sendo 30 aves/boxe, em galpão de alvenaria com acesso a um piquete de 45m², com quatro repetições. O peso corporal individual dos frangos foi medido ao nascer, aos 14, 28, 42, 56, 70 e 84 dias de idade. Para a determinação das curvas de crescimento do peso corporal das aves, os dados coletados foram avaliados por meio dos modelos não lineares: Brody, Gompertz, Logístico, Richards e von Bertalanffy. Foi empregado o PROC NLIN do SAS, utilizando-se o método interativo de Gauss-Newton. Os critérios usados para escolha do modelo de melhor ajuste da curva de crescimento foram o coeficiente de determinação, o desvio padrão assintótico, o desvio médio absoluto dos resíduos e o índice assintótico. As análises para obtenção dos coeficientes alométricos foram realizadas por meio do PROC GLM do SAS para os genótipos Carijó, Colorpak, Pesadão Vermelho, Pescoço Pelado e Tricolor. Foram avaliados os pesos da carcaça, do peito, das coxas, das sobrecoxas, das pernas e das asas das aves abatidas aos 85 dias de idade. Apenas as equações propostas por Gompertz, von Bertalanffy e Logístico atingiram a convergência, e o modelo proposto por von Bertalanffy foi o mais adequado para descrever o crescimento dos genótipos de frangos caipiras. Todos os cortes avaliados apresentaram crescimento tardio em relação ao peso da carcaça em genótipos de frangos tipo caipira.

  2. From AWE-GEN to AWE-GEN-2d: a high spatial and temporal resolution weather generator

    Science.gov (United States)

    Peleg, Nadav; Fatichi, Simone; Paschalis, Athanasios; Molnar, Peter; Burlando, Paolo

    2016-04-01

    A new weather generator, AWE-GEN-2d (Advanced WEather GENerator for 2-Dimension grid) is developed following the philosophy of combining physical and stochastic approaches to simulate meteorological variables at high spatial and temporal resolution (e.g. 2 km x 2 km and 5 min for precipitation and cloud cover and 100 m x 100 m and 1 h for other variables variable (temperature, solar radiation, vapor pressure, atmospheric pressure and near-surface wind). The model is suitable to investigate the impacts of climate variability, temporal and spatial resolutions of forcing on hydrological, ecological, agricultural and geomorphological impacts studies. Using appropriate parameterization the model can be used in the context of climate change. Here we present the model technical structure of AWE-GEN-2d, which is a substantial evolution of four preceding models (i) the hourly-point scale Advanced WEather GENerator (AWE-GEN) presented by Fatichi et al. (2011, Adv. Water Resour.) (ii) the Space-Time Realizations of Areal Precipitation (STREAP) model introduced by Paschalis et al. (2013, Water Resour. Res.), (iii) the High-Resolution Synoptically conditioned Weather Generator developed by Peleg and Morin (2014, Water Resour. Res.), and (iv) the Wind-field Interpolation by Non Divergent Schemes presented by Burlando et al. (2007, Boundary-Layer Meteorol.). The AWE-GEN-2d is relatively parsimonious in terms of computational demand and allows generating many stochastic realizations of current and projected climates in an efficient way. An example of model application and testing is presented with reference to a case study in the Wallis region, a complex orography terrain in the Swiss Alps.

  3. Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes.

    Science.gov (United States)

    Vieira Vaz, Marcelo Gomes Marçal; Genuário, Diego Bonaldo; Andreote, Ana Paula Dini; Malone, Camila Francieli Silva; Sant'Anna, Célia Leite; Barbiero, Laurent; Fiore, Marli Fátima

    2015-01-01

    The genus Leptolyngbya Anagnostidis & Komárek (1988) was described from a set of strains identified as 'LPP-group B'. The morphology within this group is not particularly informative and underestimates the group's genetic diversity. In the present study, two new pseudanabaenacean genera related to Leptolyngbya morphotypes, Pantanalinema gen. nov. and Alkalinema gen. nov., are described under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants, based on a polyphasic approach. Pantanalinema gen. nov. (type species Pantanalinema rosaneae sp. nov.) has sheaths and trichomes with slight gliding motility, which distinguish this genus from Alkalinema gen. nov. (type species Alkalinema pantanalense sp. nov.), which possesses trichomes arranged in an ornate (interwoven) pattern. 16S rRNA gene sequences of strains of Pantanalinema and Alkalinema exhibited low identity to each other (≤91.6 %) and to other sequences from known pseudanabaenacean genera (≤94.3 and 93.7 %, respectively). In a phylogenetic reconstruction, six sequences from strains of Pantanalinema and four from strains of Alkalinema formed two separate and robust clades (99 % bootstrap value), with the genera Oculatella and Phormidesmis, respectively, as the closest related groups. 16S-23S rRNA intergenic spacer sequences and secondary structures of strains of Pantanalinema and Alkalinema did not correspond to any previous descriptions. The strains of Pantanalinema and Alkalinema were able to survive and produce biomass at a range of pH (pH 4-11) and were also able to alter the culture medium to pH values ranging from pH 8.4 to 9.9. These data indicate that cyanobacterial communities in underexplored environments, such as the Pantanal wetlands, are promising sources of novel taxa.

  4. Diseño de una biblioteca de compuertas MCML utilizando un algoritmo genético y optimización multiobjetivo

    Directory of Open Access Journals (Sweden)

    Roberto Pereira-Arroyo

    2014-11-01

    Full Text Available En este documento se alude al problema de dimensionamiento de circuitos MCML (MOS Current Mode Logic. Se introduce el Frente de Pareto como una herramienta de análisis útil para explorar el espacio de diseño de las distintas compuertas que conforman nuestra biblioteca básica MCML. Un algoritmo genético (GA es implementado para detectar automáticamente este frente, en un proceso que busca eficientemente las parametrizaciones óptimas del diseño y sus correspondientes valores en un espacio de aptitudes. Las mediciones del consumo de potencia, el retardo de propagación y el rango del voltaje de salida se usan como funciones de aptitud, puesto que el problema se trata como una tarea de optimización multiobjetiva. Finalmente, se presentan los resultados de las simulaciones postlayout, usando la tecnología de fabricación AMS 0,35 μm.

  5. The Madagascan endemic myrmicine ants related to Eutetramorium (Hymenoptera: Formicidae): taxonomy of the genera Eutetramorium Emery, Malagidris nom. n., Myrmisaraka gen. n., Royidris gen. n., and Vitsika gen. n.

    Science.gov (United States)

    Bolton, Barry; Fisher, Brian L

    2014-04-24

    The monophyletic group of myrmicine ant genera related to Eutetramorium is described and its taxonomy is documented. The group is endemic in Madagascar and contains five genera: Eutetramorium Emery, 1899 (3 species, 1 of which is new); Malagidris nom. n., a replacement name for Brunella Forel, 1917, junior homonym of Brunella Smith, G.W. 1909 (Crustacea) (6 species, 5 of which are new); Myrmisaraka gen. n. (2 species, both new); Royidris gen. n. (15 species, 11 of which are new); Vitsika gen. n. (14 species, all of which are new). Keys to the worker caste are provided for all genera, and provisional keys to known males are given for Malagidris and Vitsika.

  6. A Frequency Diverse Gen2 RFID System with Isolated Continuous Wave Emitters

    Directory of Open Access Journals (Sweden)

    Hsin-Chin Liu

    2007-09-01

    Full Text Available The “Gen2” specification for UHF passive RFID systems released by EPCglobal has become an intense research interest. A Gen2 tag derives its power from the RF wave emitted by a Gen2 RFID reader and responds its modulated backscatter signals to the reader. Due to the large propagation loss, the accessible range of a Gen2 tag is hence limited. Moreover, the readability of a Gen2 tag is often influenced by the multipath fading problem. In order to mitigate the problems, a multi-carrier UHF passive RFID system utilizing the frequency diverse backscatter ability of a Gen2 tag is proposed in a prior work. In this work, a thorough analysis of the system is given. Especially, the appropriate powers of the transceiver and CWEs are derived to make the system more feasible in practical applications.

  7. Targeted Amplicon Sequencing (TAS): A Scalable Next-Gen Approach to Multilocus, Multitaxa Phylogenetics

    OpenAIRE

    2011-01-01

    Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequ...

  8. Arctocypris fuhrmanni, n. gen., n. sp. (Crustacea, Ostracoda, Eucypridinae) from Spitsbergen (Norway).

    Science.gov (United States)

    Petkovski, Trajan K; Scharf, Burkhard; Keyser, Dietmar

    2016-01-14

    Material from Spitsbergen (Norway) collected by Spitzenberger (1996) was reinvestigated. A new genus Arctocypris and a new species Arctocypris. fuhrmanni n. gen. n. sp. are described in the present paper. A key to the genera of the subfamily Eucypridinae is provided. At the moment Arctocypris n. gen. comprises four species: Arctocypris arctica (Olofsson, 1918) comb. nov.; A. dulcifons (Diebel & Pietrzeniuk, 1969) comb. nov.; A. foveata (Delorme, 1968) comb. nov. and Arctocypris fuhrmanni n. gen., n. sp.

  9. Gen 2对智能标签打印技术的影响

    Institute of Scientific and Technical Information of China (English)

    斑马公司

    2008-01-01

    @@ 从Gen 2(Class 1 Generation 2 UHF Air Interface Protocol,第二代UHF空中接口协议)技术规范批准到符合Gen 2标准的标签广泛上市,按ISO、EPCglobal Class 0、第一代(Gen 1)和专有协议制作的数以百万计的RFID标签将要投人使用.

  10. REGENERASI DAN PERBANYAKAN RUMPUT LAUT Kappaphycus alvarezii HASIL TRANSFORMASI GEN SUPEROKSIDA DISMUTASE (MaSOD)

    OpenAIRE

    Emma Suryati; Hidayah Triana; Utut Widiastuti; Andi Tenriulo

    2017-01-01

    Transformasi gen superoxide dismutase (MaSOD) pada rumput laut Kappaphycus alvarezii menggunakan Agrobacterium tumefacient telah dilakukan secara in vitro. Transformasi gen MaSOD ke dalam genom rumput laut diharapkan dapat mengurangi cekaman oksidatif terutama yang disebabkan oleh perubahan suhu, salinitas, dan cemaran logam di perairan. Penelitian ini bertujuan untuk regenerasi rumput laut hasil introduksi gen MaSOD dan non-transgenik pada labu kultur. Regenerasi dan perbanyakan rumput laut ...

  11. Recent changes in the GenBank On-line Service.

    OpenAIRE

    Benton, D

    1990-01-01

    The GenBank On-line Service provides access to the GenBank and EMBL nucleic acid sequence databases and to the Swiss-Prot and GenPept protein sequence databases. Users can query the databases by sequence similarity and annotation keywords and retrieve entries of interest. This access is available through e-mail servers, anonymous FTP, anonymous interactive login, and login to established, password-protected, individual accounts.

  12. Phen-Gen: combining phenotype and genotype to analyze rare disorders.

    Science.gov (United States)

    Javed, Asif; Agrawal, Saloni; Ng, Pauline C

    2014-09-01

    We introduce Phen-Gen, a method that combines patients' disease symptoms and sequencing data with prior domain knowledge to identify the causative genes for rare disorders. Simulations revealed that the causal variant was ranked first in 88% of cases when it was a coding variant-a 52% advantage over a genotype-only approach-and Phen-Gen outperformed other existing prediction methods by 13-58%. If disease etiology was unknown, the causal variant was assigned the top rank in 71% of simulations. Phen-Gen is available at http://phen-gen.org/.

  13. Contribuciones de Sir Roland Fisher a la Estadística Genética

    OpenAIRE

    Jaime Cuadros

    2004-01-01

    Sir Ronald Fisher (18901962) fue profesor de genética y muchas de sus innovaciones estadísticas encontraron expresión en el desarrollo de metodología en estadística genética. Sin embargo, mientras sus contribuciones en estadística matemática son fácilmente identificadas, en genética de poblaciones compartió su supremacía con Sewall Wright (1889 1988) y J. S. S. Haldane (1892 1965). Este documento muestra algunas de las mejores contribuciones de Fisher a las bases de la estadística genética, y...

  14. Seleção de genótipos parentais de acerola com base na divergência genética multivariada

    Directory of Open Access Journals (Sweden)

    CARPENTIERI-PÍPOLO VALÉRIA

    2000-01-01

    Full Text Available Este trabalho teve por objetivo identificar e selecionar genótipos parentais de acerola (Malpighia emarginata L. adequadas a programas de melhoramento genético. Nove caracteres quantitativos de maior importância agronômica foram usados para determinação da distância genética e formação de grupos similares de acessos. O agrupamento pelo método de Tocher, a partir das distâncias generalizadas de Mahalanobis, possibilitou a divisão de 14 genótipos em três grupos. Com base na divergência genética e no caráter agronômico-chave (teor de vitamina C, destacaram-se como mais promissores os cruzamentos dos genótipos: AM Mole pertencente ao grupo III, com os genótipos PR AM, N° 18, PR 17, PR 16, Eclipse, AM 22 e Dominga, todos pertencentes ao grupo I.

  15. Distancias genéticas en poblaciones del NOA

    Directory of Open Access Journals (Sweden)

    Acreche, Noemí

    1996-01-01

    Full Text Available La mayor parte de los trabajos realizados en nuestro país sobre polimorfismos hematológicos, abordan la necesaria descripción de las poblaciones. Se pone de relieve la importancia de encarar estudios, en base a la valiosa información publicada, que vinculen los grupos con técnicas que permitan realizar nuevas inferencias sobre sus relaciones. Conocidas en gran medida en cuanto a sus manifestaciones culturales, pueden aportar desde lo genético a la comprensión de los procesos microevolutivos ocurridos en una región. Para el NOA, se ha considerado la presencia de comunidades aborígenes incluídas en cuatro familias lingüísticas. Se tendrán en cuenta estos complejos como representativos de afinidades que se establecen a partir de estrechas relaciones entre las etnias, no sólo por la lengua, sino también por las características de sus sistemas productivos, religiosidad y organización. En base a las frecuencias génicas publicadas correspondientes a los siguientes alelos: I*A, I*B, I*O; M, N, S, s; Dia , Dib; P1, P2; C, c; D, d, E, e; Le, le; Fya, Fyb; Jka, Jkb; K y k se construyeron tablas de frecuencias. Se estimaron los coeficientes de distancias genéticas que fueron analizados y posteriormente incluídos en la construcción de un fenograma de los grupos de estudio, mediante agrupaciones (Sahn Cluster secuenciales, aglomerativas, jerárquicas y anidadas. De acuerdo a la información recopilada de las frecuencias de los 25 alelos estudiados en trece poblaciones de aborígenes del NOA y Paraguay, las distancias genéticas obtenidas reflejan los caracteres lingüístico-culturales.

  16. Alimentos genéticamente alterados: transgénicos

    OpenAIRE

    García González, Jaime

    2016-01-01

    Se definen los términos relacionados directamente con la temática del artículo para luego citar algunas de las contradicciones de los promotores de este tipo de alimentos. Posteriormente, se mencionan los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos genéticamente alterados. Además, se destacan las principales incertidumbres y cuestionamientos señalados y reconocidos por parte de diversos autores y organizaciones de profesionales en la salud, entre ellas...

  17. Cleaning the GenBank Arabidopsis thaliana data set

    DEFF Research Database (Denmark)

    Korning, Peter G.; Hebsgaard, Stefan M.; Rouze, Pierre;

    1996-01-01

    extracted a data set from the A. thaliana entries in GenBank. A number of simple `sanity' checks, based on the nature of the data, revealed an alarmingly high error rate. More than 15% of the most important entries extracted did contain erroneous information. In addition, a number of entries had directly...... common. It is proposed that the level of error correction should be increased and that gene structure sanity checks should be incorporated - also at the submitter level - to avoid or reduce the problem in the future. A non-redundant and error corrected subset of the data for A. thaliana is made available...

  18. Estudio de polimorfismos genéticos en tiroiditis autoinmune

    OpenAIRE

    Iglesias López, Rosa Ana

    2015-01-01

    [ES]Estudio epidemiológico caso-control para comprobar la hipótesis de que ciertos polimorfismos genéticos relacionados con las citosinas y con el proceso de autofagia pueden modificar el riesgo de presentar tiroiditis autoinmune. Se realiza el estudio sobre 200 pacientes con tiroiditis autoinmune reclutados en el Servicio de Endocrinología y Nutrición en el Complejo Asistencial Universitario de Salamanca, en los que se confirma la ausencia de diferencias en cuanto a características epidemiol...

  19. Estudio de polimorfismos genéticos en tiroiditis autoinmune

    OpenAIRE

    Iglesias López, Rosa Ana

    2015-01-01

    [ES]Estudio epidemiológico caso-control para comprobar la hipótesis de que ciertos polimorfismos genéticos relacionados con las citosinas y con el proceso de autofagia pueden modificar el riesgo de presentar tiroiditis autoinmune. Se realiza el estudio sobre 200 pacientes con tiroiditis autoinmune reclutados en el Servicio de Endocrinología y Nutrición en el Complejo Asistencial Universitario de Salamanca, en los que se confirma la ausencia de diferencias en cuanto a características epidemiol...

  20. Epistemología genética.

    OpenAIRE

    Gutiérrez Vásquez, Ramiro; Facultad de Psicología, Universidad Peruana Cayetano Heredia.

    2015-01-01

    Para recrear las ideas de Jean Piaget sobre su epistemología genética se ha visto por conveniente abordar el trabajo desde varios aspectos: abordar el problema de su significado y de lo que estudia; fundamentar su estatus de ciencia, característica central que la diferencia de otras posturas epistemológicas; probar que la psicología juega un rol importante al momento de analizar la ciencia. Del mismo modo, se estudia los métodos y procedimientos originales con los que Piaget abordó los temas ...

  1. Genética do autismo Genetics of autism

    OpenAIRE

    Gianna Carvalheira; Naja Vergani; Décio Brunoni

    2004-01-01

    O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.Autism is a neuropsychiatric disorder with profound family and social consequences. An extraordinary number of genetical-clinical, cytogenetics and molecular studies we...

  2. TrayGen: Arranging objects for exhibition and packaging

    KAUST Repository

    Yang, Yongliang

    2013-10-01

    We present a framework, called TrayGen, to generate tray designs for the exhibition and packaging of a collection of objects. Based on principles from shape perception and visual merchandising, we abstract a number of design guidelines on how to organize the objects on the tray for the exhibition of their individual features and mutual relationships. Our framework realizes these guidelines by analyzing geometric shapes of the objects and optimizing their arrangement. We demonstrate that the resultant tray designs not only save space, but also highlight the characteristic of each object and the inter-relations between objects. © 2013 The Eurographics Association and John Wiley & Sons Ltd.

  3. Enfermedades genéticas más frecuentes en pacientes atendidos en consulta de genética clínica

    OpenAIRE

    Elibett Carcasés Carcasés; Nora María Orive Rodríguez; Lisset del Carmen Romero Portelles; Glenys Katiuska Silva González

    2015-01-01

    La estimación de la prevalencia de las enfermedades genéticas se dificulta, entre otras causas, por su rareza. Se realizó un estudio descriptivo retrospectivo, para identificar las enfermedades genéticas de mayor prevalencia en pacientes atendidos por este programa en el Centro Provincial de Genética Médica de Las Tunas, Cuba; desde el año 1989 hasta julio de 2014. Se revisaron todas las historias clínicas. Predominó el origen monogénico (69 %), siendo los síndromes dismórficos los más numero...

  4. Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus.

    Science.gov (United States)

    Busse, Hans-Jürgen

    2016-01-01

    In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and polar lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, polar lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and polar lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species).

  5. Moureanthidium, gen.n. de Dianthidiini do Brasil (Hymenoptera, Megachilidae Moureanthidium, gen.n. de Dianthidiini from Brazil (Hymenoptera, Megachiliiae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    1995-01-01

    Full Text Available Moureanthidium, gen.n. is proposed for Dianthidium subarenarium Schwarz, 1933 and to include four new species of Anthidiine bees from Brazil, Moureanthidium paranaense, sp.n from Ponta Grossa, Paraná, Moureanthidium capixaba, sp.n. from Santa Teresa, Espírito Santo, Moureanthidium bahianum, sp.n. from Vitória da Conquista, Bahia and Moureanthidium catarinense, sp.n. from Seara, Santa Catarina. The males are characterized by the border of the seventh tergum strongly reflexed, densely hairy and with a pair of lateral projections and the third sternite with two spines or little projections at the middle, near the apex.

  6. Genética y genómica enfocadas en el estudio de la resistencia bacteriana

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  7. GenGIS: A geospatial information system for genomic data

    Science.gov (United States)

    Parks, Donovan H.; Porter, Michael; Churcher, Sylvia; Wang, Suwen; Blouin, Christian; Whalley, Jacqueline; Brooks, Stephen; Beiko, Robert G.

    2009-01-01

    The increasing availability of genetic sequence data associated with explicit geographic and ecological information is offering new opportunities to study the processes that shape biodiversity. The generation and testing of hypotheses using these data sets requires effective tools for mathematical and visual analysis that can integrate digital maps, ecological data, and large genetic, genomic, or metagenomic data sets. GenGIS is a free and open-source software package that supports the integration of digital map data with genetic sequences and environmental information from multiple sample sites. Essential bioinformatic and statistical tools are integrated into the software, allowing the user a wide range of analysis options for their sequence data. Data visualizations are combined with the cartographic display to yield a clear view of the relationship between geography and genomic diversity, with a particular focus on the hierarchical clustering of sites based on their similarity or phylogenetic proximity. Here we outline the features of GenGIS and demonstrate its application to georeferenced microbial metagenomic, HIV-1, and human mitochondrial DNA data sets. PMID:19635847

  8. Next gen wavelets down-sampling preserving statistics

    Science.gov (United States)

    Szu, Harold; Miao, Lidan; Chanyagon, Pornchai; Cader, Masud

    2007-04-01

    We extend the 2 nd Gen Discrete Wavelet Transform (DWT) of Swelden to the Next Generations (NG) Digital Wavelet Transform (DWT) preserving the statistical salient features. The lossless NG DWT accomplishes the data compression of "wellness baseline profiles (WBP)" of aging population at homes. For medical monitoring system at home fronts we translate the military experience to dual usage of veterans & civilian alike with the following three requirements: (i) Data Compression: The necessary down sampling reduces the immense amount of data of individual WBP from hours to days and to weeks for primary caretakers in terms of moments, e.g. mean value, variance, etc., without the artifacts caused by FFT arbitrary windowing. (ii) Lossless: our new NG_DWT must preserve the original data sets. (iii) Phase Transition: NG_DWT must capture the critical phase transition of the wellness toward the sickness with simultaneous display of local statistical moments. According to the Nyquist sampling theory, assuming a band-limited wellness physiology, we must sample the WBP at least twice per day since it is changing diurnally and seasonally. Since NG_DWT, like the 2 nd Gen, is lossless, we can reconstruct the original time series for the physicians' second looks. This technique of NG_DWT can also help stock market day-traders monitoring the volatility of multiple portfolios without artificial horizon artifacts.

  9. Enfermedades genéticas del ADN mitocondrial humano

    Directory of Open Access Journals (Sweden)

    Solano Abelardo

    2001-01-01

    Full Text Available Las enfermedades mitocondriales son un grupo de trastornos que están producidos por un fallo en el sistema de fosforilación oxidativa (sistema Oxphos, la ruta final del metabolismo energético mitocondrial, con la consiguiente deficiencia en la biosíntesis del trifosfato de adenosina (ATP, por sus siglas en inglés. Parte de los polipéptidos que componen este sistema están codificados en el ácido desoxirribonucleico (DNA mitocondrial y, en los últimos años, se han descrito mutaciones que se han asociado con síndromes clínicos bien definidos. Las características genéticas del DNA mitocondrial, herencia materna, poliplasmia y segregación mitótica, confieren a estas enfermedades propiedades muy particulares. Las manifestaciones clínicas de estas enfermedades son muy heterogéneas y afectan a distintos órganos y tejidos por lo que su correcto diagnóstico implica la obtención de datos clínicos, morfológicos, bioquímicos y genéticos. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html

  10. Interação genótipo x ambiente em genótipos de feijão-caupi semiprostrado via modelos mistos

    Directory of Open Access Journals (Sweden)

    Francisco Eduardo Torres

    2015-01-01

    Full Text Available A metodologia de modelos mistos (REML/BLUP tem sido empregada para estudar os efeitos da interação genótipo x ambiente (G x E em várias culturas, como: arroz, feijão, cana-de-açúcar, cajueiro e eucalipto, porém ainda não foi aplicada em feijão-caupi. Assim, o objetivo deste trabalho foi selecionar simultaneamente genótipos de feijão-caupi semiprostrado cultivados no Estado do Mato Grosso do Sul, via modelos mistos, que reúnam alta adaptabilidade, estabilidade e produtividade de grãos. Foram conduzidos quatro ensaios de valor de cultivo e uso com genótipos de genótipos de feijão-caupi nos anos de 2005 e 2006 em Aquidauana, Chapadão do Sul e Dourados. O delineamento experimental utilizado foi o de blocos completos casualizados, com 20 genótipos e 4 repetições. Os parâmetros genéticos foram estimados pela metodologia REML/BLUP, e a seleção baseou-se no método da média harmônica do desempenho relativo dos valores genéticos (MHPRVG, em três estratégias: seleção com base no valor genético predito, tendo-se considerado o desempenho médio dos genótipos em todos os ambientes (sem efeito de interação ou o desempenho em cada ambiente (com efeito da interação; e seleção simultânea quanto à produtividade de grãos, estabilidade e adaptabilidade. Os genótipos BRS Paraguaçu, MNC99-542F-5 e MNC99-508G-1 podem ser cultivados em vários ambientes, pois reúnem alta produtividade de grãos, adaptabilidade e estabilidade. A herdabilidade da média dos genótipos apresentou magnitude variando de moderada a alta, fato que indica excelentes possibilidades para a seleção, permitindo acurácia seletiva de 82%.

  11. Update History of This Database - GenLibi | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us GenLibi Up...date History of This Database Date Update contents 2014/03/25 GenLibi English archive site is open...base Database Description Download License Update History of This Database Site Policy | Contact Us Up

  12. How is the Xpert MRSA Gen 3 assay (Cepheid) performing on pooled eSwab medium?

    Science.gov (United States)

    Jonckheere, Stijn; Van Vaerenbergh, Kristien; Boel, An; Vankeerberghen, Anne; De Beenhouwer, Hans

    2015-11-01

    The performance of the Xpert MRSA Gen 3 was compared to the Xpert MRSA on pooled eSwab media from nose, throat, and perineum using broth enriched cultured as gold standard. A lower specificity was found for the Xpert MRSA Gen 3 compared to the Xpert MRSA (91.8% versus 97.9%; P<0.05).

  13. Pseudolimia n. gen., a new monotypic genus for Limia heterandria Regan, 1913 (Teleostei: Poeciliidae)

    NARCIS (Netherlands)

    Poeser, Fred N.

    2002-01-01

    A new genus, Pseudolimia n. gen. is established for the South American Limia heterandria Regan, 1913. It is compared in an anatomical analysis with several nominal taxa of the tribe Poeciliini. Based on number and shape of the gonapophyses, Pseudolimia n. gen. is assigned to the tribe Poeciliini.

  14. A 48-plex autosomal SNP GenPlex™ assay for human individualization and relationship testing

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Børsting, Claus; Morling, Niels

    2012-01-01

    SNPs are being increasingly used by forensic laboratories. Different platforms have been developed for SNP typing. We describe the GenPlex™ HID system protocol, a new SNP-typing platform developed by Applied Biosystems where 48 of the 52 SNPforID SNPs and amelogenin are included. The GenPlex™ HID...

  15. 77 FR 54648 - Seventh Meeting: RTCA NextGen Advisory Committee (NAC)

    Science.gov (United States)

    2012-09-05

    ... Passport Country of Citizenship Birthdate and Place of Birth (city and country) Passport and Visa Numbers... Implementing Metroplex capabilities Environmental Issues Impacting NextGen Implementation A background briefing by FAA Environmental Office on the Environmental review process for NextGen capabilities and...

  16. Alteraciones en el electrorretinograma de los pacientes con distrofia muscular de duchenne y su relación con las deleciones del gen responsable de la enfermedad Alterations in electroretinography of patients with Duchenne muscular dystrophy and their relationship with deletions of gene of the disease

    Directory of Open Access Journals (Sweden)

    Mayra Rodríguez Hernández

    1999-12-01

    Full Text Available La distrofia muscular tipo Duchenne es la más común y grave de las distrofias musculares, que afecta a uno de cada 3,500 varones nacidos vivos. La alta frecuencia con que han sido reportadas alteraciones en el electrorretinograma (ERG en pacientes con distrofia muscular de Duchenne (DMD, demuestra una posible relación entre la actividad eléctrica de la retina y las mutaciones presentes en el gen DMD que codifica para la proteína distrofina, responsable del fenotipo DMD. La mutación más común del gen DMD son las deleciones del 70 % de los pacientes, y se presentan con una gran heterogeneidad al igual que el ERG, por lo que nos propusimos comparar estas variables para conocer si están o no relacionadas. Se estudiaron 22 pacientes con manifestaciones clínicas de DMD desde el punto de vista molecular y electrorretinográfico para lo cual se utilizó las técnicas de reacción en cadena de la polimerasa para chequear 18 regiones del gen, y el registro electrorretinográfico mesópico. El 69 % de los casos presentaron deleciones, las cuales varían en extensión y ubicación de las cuales el 80 % se encontraba en el extremo 3' del gen. Predominó el ERG de tipo negativo con marcada disminución de la onda B y A en límite inferior normal o también disminuida, con relación b/a menor de uno. Este tipo de ERG, estuvo relacionado con la presencia de deleción (p= 0,027 y su ubicación en el extremo 3' del gen (p= 0,03, con un nivel de significación de 0,05, por lo que se recomienda el estudio electrorretinográfico como un elemento para reforzar el diagnóstico y sospechar el sitio de la deleción.Duchenne muscular dystrophy is the most common and serious of all muscular distropies affecting one in 3 500 males born live. The high frequency of reported alterations in electroretinographies of patients with Duchenne muscular dystrophy (DMD proves that there is a possible relationship between the electrical activity of retina and the DMD gen

  17. ANALISIS SEKUEN GEN GLUTATION PEROKSIDASE (GPX1 SEBAGAI DETEKSI STRES OKSIDATIF AKIBAT INFEKSI MYCOBACTERIUM TUBERCULOSIS

    Directory of Open Access Journals (Sweden)

    Ari Yuniastuti

    2013-02-01

    Full Text Available Glutation merupakan antioksidan yang berperan dalam fungsi imun, dan diekspresikan secara genetik oleh urutan gen yang membentuk protein enzim Glutation Peroxidase (GPx1. Bila ekspresi gen berubah maka terjadi perubahan fungsi glutation dan kerentanan terhadap stress oksidatif. Metode yang digunakan adalah Kasus-kontrol. Sampel yang digunakan adalah sampel darah. Kelompok kasus adalah sampel darah pasien tuberkulosis paru sedangkan kelompok kontrol adalah sampel darah orang sehat. Pemeriksaan gen Glutation peroxidase (GPx1 menggunakan metode Polymerase Chain Reaction (PCR untuk melihat pita DNA pada pasien tuberkulosis par serta elektroforesis produk PCR-RFLP gen GPx1 kelompok sampel tuberkulosis. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang bermakna antara polimorfisme gen GPx1 (p=0,365 pasein tuberkulois dengan individu sehat, sehingga tidak dapat digunakan sebagai alat deteksi kerentanan terhadap stress oksidatif pada pasien tuberkulosis. Perlu penelitian lanjutan yang menggunakan sampel lebih besar dan populasi etnik yang berbeda.

  18. Polimorfisme Gen Apolipoprotein E Pada Penderita Sindrom Down Trisomi 21

    Directory of Open Access Journals (Sweden)

    Malinda Meinapuri

    2013-01-01

    Full Text Available AbstrakLatar Belakang: Sindrom Down merupakan suatu kelainan kromosom yang ditandai dengan adanya baik seluruhnya (trisomi 21 maupun sebagian (translokasi suatu salinan tambahan kromosom ke 21. Apolipoprotein E (APOE merupakan suatu bentuk protein polimorfik yang disandikan oleh suatu gen yang berlokasi pada lengan panjang kromosom 19 pada posisi 13.2 (19q13.2. Polimorfism gen APOE berkaitan dengan meningkatnya frekuensi alel ε4 yang berakibat terjadinya hambatan dalam percabangan dan pertumbuhan neuron. Dimungkinkan, penderita Sindrom Down Trisomi 21 memiliki gen APOE yang berbeda dengan kontrol sebagai faktor yang dapat mengakibatkan penuaan dini otak. Metode : Penelitian ini merupakan penelitian kasus kontrol untuk mengamati perbedaan distribusi dan frekuensi alel dan genotip gen APOE pada penderita Sindrom Down trisomi 21 dibandingkan dengan kontrol. Kasus Sindrom Down dan kontrol diambil dari data sekunder yang tersimpan di Center for Biomedical Research (CEBIOR Semarang Indonesia. Ekstraksi DNA dilakukan dengan menggunakan metode yang terdapat di (CEBIOR Semarang Indonesia. Kegiatan selanjutnya adalah pemeriksaan polimorfisme gen Apolipoprotein E dengan mengunakan teknik PCR dan RFLP. Hasil : Sebanyak 33 sampel dari penderita Sindrom Down, 18 laki-laki dan 15 perempuan dan 33 sampel kontrol, 18 laki-laki dan 15 perempuan. Baik sampel Sindrom Down maupun kontrol memiliki frekuensi alel ε3 paling tinggi dibandingkan dengan alel ε2 dan ε4. Pada Sindrom Down didapatkan alel ε4 4 sampel (6,1% dan alel ε2 8 sampel (12,1%. Baik sampel Sindrom Down maupun kontrol memiliki genotip ε3/ε3 paling tinggi dibandingkan dengan genotip gen APOE lainnya. Pada Sindrom Down didapatkan genotip ε2/ε4 4 sampel (12,1% dan genotip ε2/ε2 2 sampel (6,1%. Simpulan : Terdapat perbedaan distribusi alel dan genotip gen APOE pada penderita Sindrom Down trisomi 21 dibandingkan dengan kelompok kontrol. Diperlukan analisis sampel yang lebih banyak untuk

  19. Genética da resistência à ferrugem-da-folha em aveia

    Directory of Open Access Journals (Sweden)

    Cruz Renata Pereira da

    2001-01-01

    Full Text Available A ferrugem-da-folha (Puccinia coronata f. sp. avenae é a principal doença da cultura da aveia (Avena sativa L., e o uso de cultivares resistentes é o método de controle mais importante. Este trabalho teve por objetivo determinar o controle genético da resistência à ferrugem-da-folha em aveia e identificar fontes de genes diferentes para resistência a esta doença. Foram utilizados três genótipos resistentes (UFRGS 15, UFRGS 881920 e UFRGS 86A1194-2, três genótipos suscetíveis (UFRGS 7, UFRGS 8 e UFRGS 14 e a geração segregante F3 proveniente dos cruzamentos entre estes genótipos. As plantas foram avaliadas individualmente quanto à presença ou ausência da ferrugem-da-folha, sendo os dados destas leituras utilizados numa análise genética em que a hipótese de um ou dois genes de resistência foi testada pelo qui-quadrado. Os resultados evidenciaram um gene dominante de resistência no genótipo UFRGS 881920 e dois genes complementares no genótipo UFRGS 15 quando estes foram cruzados com os suscetíveis. A análise genética feita não permitiu determinar se estes dois genótipos são ou não a mesma fonte genética de resistência.

  20. Reflexo da interação genótipo x ambiente sobre o melhoramento genético de feijão

    Directory of Open Access Journals (Sweden)

    Thayse Cristine Vieira Pereira

    2015-01-01

    Full Text Available RESUMO: O objetivo foi avaliar os componentes da variância fenotípica e estimar a influência da interação genótipo*ambiente no rendimento de grãos em feijão. Os componentes da variância fenotípica foram estimados pelo método da máxima verossimilhança restrita e do melhor preditor linear não viesado (REML/BLUP, juntamente com o espaço de inferência específico. As avaliações foram realizadas nas safras agrícolas de 2006/07 a 2011/12 no município de Lages/SC. Durante o período, 104 genótipos foram avaliados. Os dados são desbalanceados, sendo que 13 genótipos permaneceram nos ensaios em todos os anos. Observando os resultados, foi possível visualizar que a grande variação (59,0% no comportamento dos genótipos ao longo dos anos é atribuída principalmente à variância do ambiente (σ2a=436.245. Houve diferença significativa entre genótipos para todos os ambientes. Porém, a diferença entre eles foi constante, ou seja, os genótipos não responderam de modo diferenciado frente aos ambientes. A interação genótipo*ambiente (σ2ga=1.368 responde preponderantemente por uma ínfima alteração (0,2% na variação fenotípica, não discriminando de genótipos de feijão quanto ao rendimento de grãos em Lages/SC. Este fato favorece programas de melhoramento vegetal, onde a interação genótipo*ambiente poderia dificultar a distinção, seleção e recomendação de constituições genotípicas superiores. Nessa situação, processos de recomendação de cultivares (ensaio de valor de cultivo e uso - VCU que mantenham os mesmos genótipos dispensam avaliações sucessivas, pois o ranqueamento é paralelo no decorrer dos anos.

  1. Genética poblacional del caracol rosado Strombus gigas en la Península de Yucatán: Implicaciones para su manejo y pesquería

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available Se determinó la estructura genética poblacional del caracol rosado Strombus gigas en la Península de Yucatán, México, mediante la expresión de isoenzimas en geles de poliacrilamida. Se utilizaron muestras de músculo de 50 organismos, capturados en cada uno de los cuatro sitios de la Península de Yucatán seleccionados, para caracterizar la expresión genotípica revelada por la expresión de 55 loci en 30 sistemas enzimáticos. Se utilizó el programa TFPGA versión 1.3 para procesar los datos de frecuencias génicas de aloenzimas de las poblaciones en estudio. Los parámetros determinados fueron: estadística descriptiva, estadística F, distancias genéticas, equilibrio de Hardy-Weinberg, UPGMA y el número de migrantes como indicador del flujo de genes. Los valores de heterocigosis, en un rango de 0.3240 para OCTDH 2 hasta 0.0440 para FUM y promedio de 0.0366, los de Fis, con un rango de 0.0835 para OCTDH 2 hasta 0.3600 para la FUM y promedio de –0.0492, y los de Fst, con un rango de 0.0082 para LAP 2 hasta 0.1967 para MDH 2 y promedio de 0.1039, indican una deficiencia de heterocigotos. El número de migrantes derivado de la ecuación de Slatkin resultó de 2.156 por generación, lo que en forma global indica un cierto grado de variabilidad entre las poblaciones y es consistente con los bajos valores de distancia genética de Nei encontrados, particularmente en el nodo que sugiere la separación de la población de Alacranes de las otras poblaciones estudiadas, con un valor obtenido de 0.0053. Por los resultados, se concluye que las poblaciones de S. gigas en el Caribe mexicano presentan niveles de variabilidad genética que no reflejan fragilidad para su subsistencia.

  2. GenASiS: General Astrophysical Simulation System. I. Fundamentals

    CERN Document Server

    Cardall, Christian Y; Endeve, Eirik; Mezzacappa, Anthony

    2012-01-01

    GenASiS (General Astrophysical Simulation System) is a new code being developed initially and primarily, though by no means exclusively, for the simulation of core-collapse supernovae on the world's leading capability supercomputers. Using the features of Fortran 2003 that allow for object-oriented programming, its classes are grouped into three major divisions: (1) Basics, which contains some basic utilitarian functionality for large-scale simulations on distributed-memory supercomputers; (2) Mathematics, which includes generic mathematical constructs and solvers that are as agnostic as possible with regard to the specifics of any particular system; and (3) Physics, which sets up physical spaces associated with various theories of spacetime (including gravity), defines various forms of stress-energy, and combines these into `universes.' To provide a foundation for subsequent papers focusing on the implementation of various pieces of physics needed for the simulation of core-collapse supernovae and other astr...

  3. International Conference on NextGen Electronic Technologies

    CERN Document Server

    Thalmann, Nadia; Bhaaskaran, V

    2017-01-01

    This book is a collection of keynote lectures from international experts presented at International Conference on NextGen Electronic Technologies (ICNETS2-2016). ICNETS2 encompasses six symposia covering all aspects of electronics and communications domains, including relevant nano/micro materials and devices . This volume comprises of recent research in areas like computational signal processing analysis, intelligent embedded systems, nanoelectronic materials and devices, optical and microwave technologies, VLSI design: circuits systems and application, and wireless communication networks, and the internet of things. The contents of this book will be useful to researchers, professionals, and students working in the core areas of electronics and their applications, especially to signal processing, embedded systems, and networking.

  4. What Can a Historian Do with AstroGen?

    Science.gov (United States)

    Tenn, Joseph S.

    2015-01-01

    "Astrogen", the Astronomy Genealogy Project, is in the development stage. Patterned after the Mathematics Genealogy Project at http://genealogy.math.ndsu.nodak.edu, it will eventually include most of the world's astronomers, past and present, and provide information about their years of life, highest degrees, universities, and thesis titles. There will also be links to online theses, home pages, and obituaries when these are available. Although a few details remain to be worked out before it becomes public, it is possible to make some use of what has already been compiled. I will give an example, comparing graduates of Harvard University, the University of California at Berkeley, and the University of Chicago from different decades, with information about their professional careers and publication records. The author welcomes queries about AstroGen and is seeking more participants.

  5. O sujeito na epistemologia genética

    OpenAIRE

    Abib,José Antônio Damásio

    2003-01-01

    Sugere-se neste ensaio que, na epistemologia genética, o sujeito se dirige ao mundo, se interessa por ele e é um princípio de auto-organização. Ontologicamente, seu modo de existência é o do encontro: encontro com o mundo. Epistemologicamente, seu modo de conhecimento é o da presença: presença do mundo. O construtivismo, a tese de que o conhecimento é construção de relações, começa como presença, o que exclui a noção de conhecimento como representação. Destaca-se a centralidade da noção de au...

  6. How Gen Y and Boomers will reshape your agenda.

    Science.gov (United States)

    Hewlett, Sylvia Ann; Sherbin, Laura; Sumberg, Karen

    2009-01-01

    When it comes to workplace preferences, Generation Y workers closely resemble Baby Boomers. Because these two huge cohorts now coexist in the workforce, their shared values will hold sway in the companies that hire them. The authors, from the Center for Work-Life Policy, conducted two large-scale surveys that reveal those values. Gen Ys and Boomers are eager to contribute to positive social change, and they seek out workplaces where they can do that. They expect flexibility and the option to work remotely, but they also want to connect deeply with colleagues. They believe in employer loyalty but desire to embark on learning odysseys. Innovative firms are responding by crafting reward packages that benefit both generations of workers--and their employers.

  7. Commissioning and Performance Analysis of WhisperGen Stirling Engine

    Science.gov (United States)

    Pradip, Prashant Kaliram

    Stirling engine based cogeneration systems have potential to reduce energy consumption and greenhouse gas emission, due to their high cogeneration efficiency and emission control due to steady external combustion. To date, most studies on this unit have focused on performance based on both experimentation and computer models, and lack experimental data for diversified operating ranges. This thesis starts with the commissioning of a WhisperGen Stirling engine with components and instrumentation to evaluate power and thermal performance of the system. Next, a parametric study on primary engine variables, including air, diesel, and coolant flowrate and temperature were carried out to further understand their effect on engine power and efficiency. Then, this trend was validated with the thermodynamic model developed for the energy analysis of a Stirling cycle. Finally, the energy balance of the Stirling engine was compared without and with heat recovery from the engine block and the combustion chamber exhaust.

  8. Festival du rire de Genève

    CERN Multimedia

    Staff Association

    2015-01-01

    Connaissez-vous le Festival du rire de Genève ? La deuxième édition aura lieu du 25 au 28 mars 2015 au Casino-Théâtre à Carouge. Côté programmation, Marc Donnet-Monay ouvre les festivités avant trois autres soirées de folie et d’humour que nous vous laissons le soin de découvrir dans le programme : http://www.rire-geneve.ch/#programme. Réduction de 30% sur l’achat de places pour les membres du personnel du CERN. Pour cela, il suffit de se rendre sur la billetterie en ligne de notre site : www.rire-geneve.ch et d’utiliser le code promotionnel. Contacter le secrétariat de l’Association du personnel (Staff.Association@cern.ch) pour connaitre ce code promotionnel.

  9. Genética de las epilepsias Genetics of epilepsy

    Directory of Open Access Journals (Sweden)

    Gustavo A. Charria-Ortiz

    2007-01-01

    Full Text Available En años recientes se ha podido definir con gran exactitud la existencia de alteraciones genéticas específicas en una gran variedad de síndromes epilépticos tradicionales. Es decir, por vez primera se ha podido relacionar de manera contundente y predecible la presencia de alteraciones genómicas y/o proteómicas con síndromes epilépticos antes considerados como "idiopáticos". La gran mayoría de dichos defectos han sido encontrados en genes codificadores para canales iónicos y/o receptores de membrana, lo cual en cierto modo confirma la ya antes postulada relevancia que estas estructuras tienen en la actividad electroquímica espontánea neuronal cuyo desajuste conllevaría a ciertas formas de epilepsia. Esta revisión se centra en los aspectos genéticos y clínicos de dichas condiciones y alteraciones. También se revisarán brevemente los estudios más relevantes de la literatura médica según los cuales -aun a pesar de no haberse definido con la misma exactitud el tipo de anomalías etiológicas- puede tranquilamente inferirse el gran componente genético que parece subyacer a la etiología de las epilepsias. Por ultimo se enfatizará en que a pesar de dichos descubrimientos, su aplicación en la práctica clínica diaria aun es muy limitada, no solo por la relativa rareza de algunos de tales síndromes neurológicos sino también por la poca relevancia que hasta ahora ellos han tenido en el manejo médico rutinario de la mayoría de los pacientes. Las posibilidades inmediatas de tales avances -incluida la farmacogenómica-, así como los posibles conflictos éticos en que se podría incurrir serán también brevemente discutidos.In the last few years, the presence of specific genetic abnormalities leading to some of the classical epileptic syndromes has been clearly elucidated. This means that for the first time, it has become possible to create a strong relationship between the presence of specific genomic and/or proteomic

  10. Genética do autismo Genetics of autism

    Directory of Open Access Journals (Sweden)

    Gianna Carvalheira

    2004-12-01

    Full Text Available O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.Autism is a neuropsychiatric disorder with profound family and social consequences. An extraordinary number of genetical-clinical, cytogenetics and molecular studies were done in recent years. A multiloci epistatic model involved in the causation of autism have emerged from these studies.

  11. New Boletaceae taxa from Guyana: Binderoboletus segoi gen. and sp. nov., Guyanaporus albipodus gen. and sp. nov., Singerocomus rubriflavus gen. and sp. nov., and a new combination for Xerocomus inundabilis.

    Science.gov (United States)

    Henkel, Terry W; Obase, Keisuke; Husbands, Dillon; Uehling, Jessie K; Bonito, Gregory; Aime, M Catherine; Smith, Matthew E

    2016-01-01

    Binderoboletus segoi gen. and sp. nov., Guyanaporus albipodus gen. and sp. nov. and Singerocomus rubriflavus gen. and sp. nov. (Boletaceae, Boletales, Basidiomycota) are described from the Pakaraima Mountains and adjacent lowlands of Guyana. Xerocomus inundabilis, originally described from the central Brazilian Amazon and based solely on the type collection, is redescribed from numerous collections from Guyana and transferred into Singerocomus. These boletes occur in Neotropical forests dominated by ectomycorrhizal trees in the genera Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae) and Pakaraimaea (Dipterocarpaceae). Three of the species were repeatedly found in a multiyear sporocarp survey in Dicymbe corymbosa-monodominant forest. Macromorphological, micromorphological, habitat and multilocus DNA sequence data are provided for each species. A molecular phylogenetic analysis based on a large taxon set across the Boletaceae justifies erection of the new genera.

  12. Hand-held optical imager (Gen-2): improved instrumentation and target detectability.

    Science.gov (United States)

    Gonzalez, Jean; Decerce, Joseph; Erickson, Sarah J; Martinez, Sergio L; Nunez, Annie; Roman, Manuela; Traub, Barbara; Flores, Cecilia A; Roberts, Seigbeh M; Hernandez, Estrella; Aguirre, Wenceslao; Kiszonas, Richard; Godavarty, Anuradha

    2012-08-01

    Hand-held optical imagers are developed by various researchers towards reflectance-based spectroscopic imaging of breast cancer. Recently, a Gen-1 handheld optical imager was developed with capabilities to perform two-dimensional (2-D) spectroscopic as well as three-dimensional (3-D) tomographic imaging studies. However, the imager was bulky with poor surface contact (~30%) along curved tissues, and limited sensitivity to detect targets consistently. Herein, a Gen-2 hand-held optical imager that overcame the above limitations of the Gen-1 imager has been developed and the instrumentation described. The Gen-2 hand-held imager is less bulky, portable, and has improved surface contact (~86%) on curved tissues. Additionally, the forked probe head design is capable of simultaneous bilateral reflectance imaging of both breast tissues, and also transillumination imaging of a single breast tissue. Experimental studies were performed on tissue phantoms to demonstrate the improved sensitivity in detecting targets using the Gen-2 imager. The improved instrumentation of the Gen-2 imager allowed detection of targets independent of their location with respect to the illumination points, unlike in Gen-1 imager. The developed imager has potential for future clinical breast imaging with enhanced sensitivity, via both reflectance and transillumination imaging.

  13. Factors genètics de la leishmaniosi canina: caracterització del Nramp1 com a gen candidat

    OpenAIRE

    Altet i Sanahujes, Laura

    2002-01-01

    Descripció del recurs: el 22 d'octubre de 2003 L'objectiu d'aquesta tesi és la recerca de gens candidats de resistència o susceptibilitat a leishmaniosi canina. Aquesta malaltia, que és endèmica en l'area mediterranea, és una zoonosi causada pel paràsit Leishmania infantum, on el gos és el principal reservori. A més, està guanyant molta importància en l'home, ja que ha esdevingut una de les coinfeccions principals en malalts del virus de la SIDA. El món dels gossos aporta un material molt ...

  14. Avaliação genética para peso corporal em um rebanho Nelore

    Directory of Open Access Journals (Sweden)

    F.M Gonçalves

    2011-02-01

    Full Text Available Estimaram-se as herdabilidades para os efeitos genéticos direto e materno e as correlações genéticas entre essas variáveis para os pesos ao desmame (P205, ao ano (P365 e ao sobreano (P550 em um rebanho Nelore do norte de Minas Gerais. O modelo estatístico incluiu os efeitos aditivos direto e materno, os efeitos fixos de grupo de contemporâneos (fazenda, sexo, regime alimentar, estação (seca e água e ano de nascimento do animal e o efeito da covariável idade da vaca ao parto (linear e quadrático. Os componentes de variância e os valores genéticos foram estimados utilizando-se o método REML. A tendência genética foi obtida utilizando-se a regressão do valor genético médio anual em relação ao ano de nascimento dos animais. As estimativas de herdabilidade do efeito aditivo direto ( para P205, P365 e P550 foram 0,60, 0,69 e 0,75, respectivamente. Estes coeficientes de são de alta magnitude, indicando que o rebanho apresenta variabilidade genética aditiva relativa e, portanto, espera-se progresso genético considerável utilizando a seleção. Pela análise da tendência genética, verificou-se que houve evolução nos valores genéticos dos animais ao longo dos anos estudados.

  15. Estructura genética poblacional del gen lactoferrina bovino en vacas Holstein del departamento de Antioquia

    Directory of Open Access Journals (Sweden)

    Nancy Rodríguez C.

    2013-03-01

    Full Text Available Objetivo. Estimar algunos parámetros de estructura poblacional en una población Holstein del departamento de Antioquia. Materiales y métodos. El estudio se realizó con 427 vacas de la raza Holstein pertenecientes a 5 municipios del departamento de Antioquia. La genotipificación se llevó a cabo usando la técnica de PCR-PFLPs. La Heterocigocidad observada (Ho y Heterocigocidad esperada (He, la prueba de Hardy-Weinberg (HW y la estructura y diferenciación genética entre las poblaciones se calculó mediante los parámetros F de Wright, evaluados mediante el software GENEPOP. Las frecuencias alélicas y genotípicas se evaluaron con el método descrito por Hartl. Resultados. Las frecuencias genotípicas encontradas fueron 0.61, 0.34 y 0.05 para los genotipos AA, AB y BB respectivamente y las frecuencias de los alelos fueron 0.78 y 0.22 para A y B, encontrándose la población en equilibrio de HW. La heterocigocidad fue media entre poblaciones (Ho=0.368. Los valores FIS, FST y FIT de la población total fueron -0.0717, 0.0099 y -0.0611. Conclusiones. No fue posible asumir endogamia, ni exogamia en los municipios analizados, exceptuando el municipio de San Pedro de los Milagros, en cuyo caso se percibe de manera más fuerte el efecto del mejoramiento genético y la disminución de la heterocigocidad.

  16. gSeaGen: a GENIE-based code for neutrino telescopes

    CERN Document Server

    Distefano, Carla

    2016-01-01

    The gSeaGen code is a GENIE based application to generate neutrino-induced events in an underwater neutrino detector. The gSeaGen code is able to generate events induced by all neutrino flavours, taking into account topological differences between track-type and shower-like events. The neutrino interaction is simulated taking into account the density and the composition of the media surrounding the detector. The main features of gSeaGen will be presented together with some examples of its application within ANTARES and KM3NeT.

  17. Determinantes genéticos y ambientales de la experiencia de burlas : un estudio gemelar

    OpenAIRE

    Iranzo Tatay, Carmen

    2014-01-01

    Muchos aspectos de la conducta humana están significativamente influenciados por factores genéticos. Examinar la heredabilidad para una exposición ambiental, lo que se ha denominado correlación gen-ambiente o control genético para la exposición ambiental, es de interés para el campo de la salud mental porque proporciona conciencia sobre la naturaleza de la relación causal entre los humanos y su entorno social y físico. Nosotros planteamos que se puede evaluar la heredabilidad de las burlas pe...

  18. gSeaGen: A GENIE-based code for neutrino telescopes

    Directory of Open Access Journals (Sweden)

    Distefano Carla

    2016-01-01

    Full Text Available The gSeaGen code is a GENIE based application to generate neutrino-induced events in an underwater neutrino detector. The gSeaGen code is able to generate events induced by all neutrino flavours, taking into account topological differences between track-type and shower-like events. The neutrino interaction is simulated taking into account the density and the composition of the media surrounding the detector. The main features of gSeaGen will be presented together with some examples of its application within ANTARES and KM3NeT.

  19. Organismos modificados genéticamente en la alimentación humana

    OpenAIRE

    Barros Fernández, Paula

    2014-01-01

    El presente trabajo trata la controversia del tema de los organismos modificados genéticamente (OMG). Se mencionan los beneficios que aporta la ingeniería genética y también los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos modificados genéticamente, reportando casos de estudios que así lo constatan.Se tratan temas como seguridad alimentaria, legislación y normativas de etiquetado de estos nuevos alimentos, señalando su relación con la salud. Además, se ...

  20. Sobre el origen del código Genético

    OpenAIRE

    Fumiyoshi Watanabe; Michelle Robles; García, José A.

    2007-01-01

    Existen ciertas evidencias experimentales que sugieren un mundo primitivo en el que la molécula de ácido ribonucleico (RNA) era la molécula responsable de codificar la información genética y de catalizar un número limitado de reacciones. En este "mundo de RNA" pudo darse el origen del código genético actual. En este manuscrito se presenta una breve discusión sobre las principales teorías que se han propuesto sobre el origen del código genético.

  1. Genética y Probabilidad : pruebas de paternidad y portadores de enfermedades

    OpenAIRE

    2006-01-01

    La genética de poblaciones estudia la distribución de los genes en las poblaciones, así como los factores que mantienen o cambian la constitución genética de las mismas, de una generación a otra. Una población se define como un conjunto de individuos capaces de reproducirse entre sí, que coexisten geográfica y temporalmente, y constituyen una unidad evolutiva. El estudio de la genética de poblaciones es muy importante desde el punto de vista sanitario, ya que la mayoría de las enfermedades ti...

  2. Consideraciones genéticas sobre las dislipidemias y la aterosclerosis

    OpenAIRE

    Julio César Fernández Travieso

    2008-01-01

    La interacción entre factores genéticos y ambientales explican muchos aspectos de la aterosclerosis y las variaciones genéticas constituyen marcadores de riesgo de la enfermedad coronaria (EC), la cual ocupa el primer lugar entre las causas de morbilidad y mortalidad a nivel mundial. La predisposición familiar a padecer EC, junto al avance vertiginoso en técnicas de análisis de ADN y la disponibilidad de secuencias del genoma humano, han orientado la investigación de alteraciones genéticas re...

  3. The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force

    Science.gov (United States)

    2001-01-01

    The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force *EDITED by DR. RICHARD H. KOHN Editorial Abstract: Air...Force chief of staff Gen Ronald Fogleman’s early retirement in 1997 has caused great speculation. Was this a “resignation in protest”? Here for the...COVERED 00-00-2001 to 00-00-2001 4. TITLE AND SUBTITLE The Early Retirement of Gen Ronald R. Fogleman, Chief of Staff, United States Air Force 5a

  4. RAPDs na caracterização genético-molecular e no estudo da variabilidade genética de cultivares de ameixeira

    Directory of Open Access Journals (Sweden)

    Bianchi Valmor João

    2003-01-01

    Full Text Available Marcadores moleculares têm sido amplamente utilizados nas mais variadas espécies frutíferas para análise de "fingerprinting", para o processo de certificação de material vegetal e como ferramenta auxiliar em programas de melhoramento genético, para acessar a variabilidade genética entre genótipos. Dado a importância da cultura da ameixeira para a região Sul do Brasil, o presente trabalho teve por finalidade contribuir para a caracterização genético-molecular de 17 cultivares. As cultivares foram analisadas com 12 marcadores RAPD, que produziram 187 polimorfismos. O marcador OP A20 foi o mais polimórfico, produzindo 26 perfis diferentes. A análise de agrupamento, realizada com o método UPGMA, produziu um dendrograma que permitiu uma clara separação das cultivares em três grupos, correspondentes às suas respectivas espécies, Prunus salicina, Prunus domestica e Prunus cerasifera. O alto grau de polimorfismo detectado pelos marcadores RAPD confirma o potencial da técnica na análise de "fingerprinting" e sua utilidade na estimativa da variabilidade genética entre cultivares de ameixeira.

  5. La introducción de la genética en México: la genética aplicada al mejoramiento vegetal

    Directory of Open Access Journals (Sweden)

    Gaona Robles, Ana Lilia

    2001-12-01

    Full Text Available The aim of this paper is to deal with the introduction, sensu Conry, of genetics in Mexico. In the XIX Century the medical community was one of the most prominent groups that developed the field of «heredity», although they never used the genetical principles in their field. We will try to show that one of the main events in the history of genetics in Mexico deals with the introduction of techniques, rather than theories. The research in genetics began through one of the most practical applications, experimental genetics applied to the improvement of species with economical interests.

    El objetivo de este artículo es estudiar la introducción, sensu Conry, de la genética en México. Para ello, hablaremos de la comunidad médica del XIX, la que desarrolló los principios fundamentales de la «herencia», sin aplicar los principios elementales de la genética. Trataremos de mostrar cómo los principios genéticos fueron introducidos vía las técnicas y no vía la teoría. La investigación genética en México comenzó a través de sus aspectos más prácticos, la genética experimental aplicada al mejoramiento de las especies con intereses económicos.

  6. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia.Genética de la esperanza

    Directory of Open Access Journals (Sweden)

    Silvio Bolaños-Salvatierra

    2003-01-01

    Full Text Available En este documento se rebaten críticas hechas por Raventós y Jensen al artículo “Genética y comportamiento”. Cuatro temas fueron seleccionados: 1 se determina que los antipsicóticos aparecieron veinte años después de la concepción hereditaria de la esquizofrenia; 2 se considera que la discusión es altamente pertinente, para nada bizantina o irrelevante, debido que persisten prácticas epistémicas riesgosas en los investigadores genético-conductuales; 3 aunque ninguna conducta humana está exenta de influencia constitucional, el enfoque biologicista se ha propasado al pretender explicar genéticamente casi todo, desconfirmando solapadamente la importancia de la historia personal; y, 4 se plantea que la investigación biológica sobrevalora el peso de las anomalías genéticas frente a la historia social, por lo que solo aparenta cautela. Se propone investigar genéticamente la esperanza con el objetivo de saturar a la humanidad con ese tipo de explicaciones, para alcanzar más rápido una convivencia basada en la tolerancia y el respeto.

  7. La transgresión genérica, emblema de la obra de Javier Marías, Enrique Vila-Matas e Ignacio Padilla

    Directory of Open Access Journals (Sweden)

    Siridia Fuertes Trigal

    2011-12-01

    Full Text Available Nowadays, the genres are not as delimited as they use to be. In Javier Marias and Enrique Vila-Matas's novels, there is continuous reflection on the role of the author, as well as on life and literature (autobiography, essay and fiction. Padilla, a member of the group of ?Mexican Crack?, chooses both Spaniers as models. In both novels Amphitryon or Espiral de artillería there are commons traits with the novels of Marías and Vila-Matas. In this paper my aim is to analyse the relationship between Padilla and these Spanish writers workEn la época actual, los límites en la literatura han desaparecido, lo que se traduce en la ruptura de las fronteras genéricas y en la composición de obras híbridas que participan de todos los géneros. Tanto la obra de Vila-Matas como la de Javier Marías se nos presentan como una continua y trabada reflexión acerca del papel del autor en la creación literaria o, lo que en parte viene a ser lo mismo, acerca de las relaciones entre vida y literatura, entre autobiografía, ensayo y ficción. El mexicano Ignacio Padilla, miembro del autodenominado grupo del Crack mexicano, ha tomado como maestros del hibridismo genérico a estos dos autores españoles, y muestra de ello es la presencia en novelas como Amphitryon o Espiral de artillería de rasgos inspirados en las novelas policíacas, ensayísticas, históricas e incluso sociales. En este trabajo analizaré los rasgos que identifican de forma clara la obra de Padilla con Javier Marías y el prolífico Enrique Vila-Matas en un continuo diálogo entre las dos orillas

  8. Variabilidad genética de la bacteria Ralstonia solanacearum (Burkholderiales: Burholderiaceae en la zona bananera de Urabá (Colombia

    Directory of Open Access Journals (Sweden)

    Carolina Cardozo

    2010-03-01

    Full Text Available La enfermedad del moko de las musáceas, causada por la bacteria Ralstonia solanacearum, es uno de los problemas fitopatológicos más importantes de la agroindustria del banano en los países tropicales. En Urabá y el Magdalena (Colombia, las principales regiones exportadoras de banano en Colombia, esta enfermedad provoca una destrucción estimada en 16.5ha/año. La bacteria presenta un nivel extremadamente alto de variación genética que afecta las medidas de control. Este es el primer estudio de su variación en Colombia y se hizo con los marcadores moleculares AFLP en una población de 100 aislamientos de plantas de banano, suelos y arvenses. El alto nivel de diversidad genética, con índices de Nei y de Shannon de h=0,32 y I=0,48, respectivamente, y la AMOVA, demostró que esta población es subestructurada (Fst=0,66: el hospedero es el principal factor de diferenciación. Aun así, las pruebas anteriores mostraron que todas las variedades presentan patogenicidad en Musa.Genetic variability of the bacterium Ralstonia solanacearum (Burkholderiales: Burholderiaceae in the banana-growing region of Uraba (Colombia. The banana moko disease, caused by the bacterium Ralstonia solanacearum, is one of the most important phytopathological problems of the banana agribusiness in tropical countries. In Uraba and Magdalena (Colombia, the main exporting regions of banana in Colombia, this disease causes a destruction estimated in 16.5ha/year. The bacterium presents an extremely high level of genetic variation that affects control measures. This is the first study of its variation in Colombia and was done with AFLP molecular markers on a population of 100 isolates from banana plants, soils and "weeds". The high level of genetic diversity, with Nei and Shannon indexes of h=0.32 and I=0.48, respectively, and the AMOVA, showed that this population is subestructured (Fst=0.66: the host is the main factor of differentiation. Even so, previous tests show that

  9. Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

    Science.gov (United States)

    Gerritsen, Jacoline; Fuentes, Susana; Grievink, Wieke; van Niftrik, Laura; Tindall, Brian J; Timmerman, Harro M; Rijkers, Ger T; Smidt, Hauke

    2014-05-01

    A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight-chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell-wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1σ lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol%. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of species of the genus Clostridium, including Clostridium lituseburense (97.2%), Clostridium glycolicum (96.2%), Clostridium mayombei (96.2%), Clostridium bartlettii (96.0%) and Clostridium irregulare (95.5%). All these species show very low 16S rRNA gene sequence similarity (gen. nov., is proposed. The novel isolate CRIBT (=DSM 25109T=NIZO 4048T) is proposed as the type strain of the type species, Romboutsia ilealis gen. nov., sp. nov., of the proposed novel genus. It is proposed that C. lituseburense is transferred to this genus as Romboutsia lituseburensis comb. nov. Furthermore, the reclassification into novel genera is proposed for C. bartlettii, as Intestinibacter bartlettii gen. nov., comb. nov. (type species of the genus), C. glycolicum, as Terrisporobacter glycolicus gen. nov., comb. nov. (type species of the genus), C. mayombei, as Terrisporobacter mayombei gen. nov., comb. nov., and C. irregulare, as Asaccharospora irregularis gen. nov., comb. nov. (type species of the genus), on the basis of additional data collected in this study. In addition, an emendation of the species Peptostreptococcus anaerobius and the order Eubacteriales is provided.

  10. Desempenho agronômico e divergência genética de genótipos de coentro Agronomic performance and genetic divergence of coriander genotypes

    Directory of Open Access Journals (Sweden)

    Cândida Hermínia de Magalhães Bertini

    2010-09-01

    Full Text Available Cinco genótipos de Coriandrum sativum, procedentes de diferentes regiões do Estado do Ceará e duas cultivares comerciais, foram avaliados objetivando-se identificar genótipos com potencial agronômico para serem utilizados em programa de melhoramento genético por meio de análise de desempenho e divergência genética. O trabalho foi conduzido no período de setembro a dezembro de 2007 no município de Fortaleza-CE. O delineamento utilizado foi o aleatorizado em blocos com três repetições, onde os genótipos e cultivares foram considerados tratamentos. Na análise de desempenho verificou-se diferença entre as médias dos genótipos avaliados para as características: altura da planta, diâmetro do colo, surgimento da primeira inflorescência, antese, média de umbeletes/umbela, início e término do amadurecimento dos frutos. Entretanto, para as características agronômicas, número de folhas aproveitáveis e peso de cem frutos, não se verificou diferença entre as médias dos genótipos avaliados em relação às cultivares comerciais. Quanto à divergência, o genótipo 1, proveniente da região litorânea de Caucaia, foi o mais divergente, podendo ser usado em cruzamentos com os demais genótipos para a obtenção de populações segregantes. Os genótipos mais similares foram o Verdão-SF177 e o genótipo proveniente de Juazeiro. Os resultados do agrupamento não mostraram relação com as diferentes localizações geográficas dos genótipos avaliados.Five genotypes of coriander (Coriandrum sativum from different regions of Ceara-Brazil and two cultivars were assessed with the objective to identify genotypes with agronomic potential for use in breeding program using performance analysis and genetic divergence. The study was conducted during the period of September to December, 2007 in Fortaleza - Ceará. It was used a randomized blocks design with three replicates where the genotypes and cultivars were considered treatments. In

  11. Aspectos genéticos da obesidade Genetics of obesity

    Directory of Open Access Journals (Sweden)

    Iva Marques-Lopes

    2004-09-01

    Full Text Available A obesidade definida como a acumulação excessiva de gordura corporal deriva de um desequilíbrio crônico entre a energia ingerida e a energia gasta. Neste desequilíbrio podem estar implicados diversos fatores relacionados com o estilo de vida (dieta e exercício físico, alterações neuro-endócrinas, juntamente com um componente hereditário. O componente genético constitui um fator determinante de algumas doenças congênitas e um elemento de risco para diversas doenças crônicas como diabetes, osteoporose, hipertensão, câncer, obesidade, entre outras. O aumento da prevalência da obesidade em quase todos os países durante os últimos anos, parece indicar que existe uma predisposição ou susceptibilidade genética para a obesidade, sobre a qual atuam os fatores ambientais relacionados com os estilos de vida, em que se incluem principalmente os hábitos alimentares e a atividade física. A utilização de modelos animais de obesidade, a transferência génica e os estudos de associação e ligamento, permitiram a identificação de vários genes implicados na obesidade.Obesity, defined as an excessive body fat accumulation, is caused by a chronic imbalance between energy intake and energy expenditure. Several factors have been associated with this energy imbalance, such as life style (diet and physical activity, neuroendocrine disorders, together with the genetic background. The genetic background is a major determinant factor of some congenital diseases and a risk factor for some chronic disorders, such as diabetes, osteoporosis, hypertension, cancer, obesity, and others. The increased prevalence of obesity in most countries during the last years, seems to indicate that there is a genetic predisposition or susceptibility to be obese which is increased by environmental and life style factors, mainly by food habits and physical in activity. The use of obesity animal models, genetic transfer and the association and linkage studies

  12. Genética y Arqueología : Análisis molecular de ADN procedente de restos esqueléticos.

    Directory of Open Access Journals (Sweden)

    N. Izaguirre

    1998-01-01

    Full Text Available La recuperación de moléculas de ADN mitocondrial (ADNmt de   muestras esqueléticas de diversa antigüedad, permite estudiar la   variabilidad genética de los grupos humanos del pasado. El análisis del ADNmt en tejido antiguo presenta una ventaja fundamental frente al nuclear, debido a su alto número de copias por célula. Los principales problemas metodológicos descritos en este tipo de   análisis, han podido resolverse en aquellos casos en que las   muestras presentaban buenas condiciones de preservación. Estos han sido fundamentalmente : 1.- inhibición de la amplificación,2.- autentificación de los resultados y 3.- detección y   eliminación de la contaminación. La variabilidad en el éxito de la amplificación entre individuos de un mismo yacimiento, indica que las condiciones individuales de cada inhumación son más importantes que la antigüedad. Por otro lado, las piezas dentarias han demostrado ser el material más adecuado para el estudio del ADNmt antiguo. La interpretación de los datos a nivel poblacional sólo es posible en aquellos yacimientos que presentan un amplio número de individuos, dado el relativamente bajo rendimiento de la metodología.

  13. GenASiS: General Astrophysical Simulation System. II. Nonrelativistic Hydrodynamics

    CERN Document Server

    Cardall, Christian Y; Endeve, Eirik; Mezzacappa, Anthony

    2012-01-01

    In this paper, the second in a series, we document the algorithms and solvers for compressible nonrelativistic hydrodynamics implemented in GenASiS (General Astrophysical Simulation System)---a new code being developed initially and primarily, though by no means exclusively, for the simulation of core-collapse supernovae. In the Mathematics division of GenASiS we introduce Solvers, which includes finite-volume updates for generic hyperbolic BalanceEquations and ordinary differential equation integration Steps. We also introduce the Physics division of GenASiS; this extends the Manifolds division of Mathematics into physical Spaces, defines StressEnergies, and combines these into Universes. We benchmark the hydrodynamics capabilities of GenASiS against many standard test problems; the results illustrate the basic competence of our implementation, demonstrate the manifest superiority of the HLLC over the HLL Riemann solver in a number of interesting cases, and provide preliminary indications of the code's abili...

  14. Ekspresi Gen CYP19 Aromatase, Estrogen, Androgen pada penderita Periodontitis Agresif

    Directory of Open Access Journals (Sweden)

    Dahlia Herawati

    2016-11-01

    Full Text Available Kepadatan tulang tubuh ditentukan oleh gen CYP19 aromatase, hormon estrogen dan androgen. Pada periodontitis agresif terjadi perkembangan cepat kerusakan tulang alveolar, dan kerusakan tulang alveoler tersebut tidak diimbangioleh regenerasi tulang. Tujuan penelitian ini adalah menunjukkan ekspresi gen CYP19 aromatase, estrogen, androgen pada penderita periodontitis agresif agar dapat untuk menjadi pertimbangan pada saat melakukan perawatan periodontal. Metode penelitian, pemeriksaan ekspresi gen aromatse CYP19 berasal dari spesimen tulang alveolar menggunakan imunohistokimia, pengukuran hormon estrogen dan androgen dari serum menggunakan Vidas: Elfa. Hasil penelitian ekspresi gene CYP19 aromatase pada periodontitis agresif menunjukkan gambaran lebih rendah densitasnya dibandingkan pada nonperiodontitis. Estrogen dan androgen pad aperiodontitis agresif ada kecenderungan lebih rendah dibandingkan pada nonperiodontitis. Kesimpulan regenerasi tulang alveoler pad a periodontitis agresif terhambat karena sedikitnya gen CYP19 aromatase dan hormon estrogen dan androgen yang berperan pada pembentukan tulang alveoler kurang memadai.

  15. On the new monotypic wolf spider genus Ovia gen. nov. (Araneae: Lycosidae, Lycosinae).

    Science.gov (United States)

    Sankaran, Pradeep M; Malamel, Jobi J; Sebastian, Pothalil A

    2017-01-17

    A new monotypic wolf spider genus, Ovia gen. nov. is proposed to accommodate a misplaced species: Pardosa procurva Yu & Song, 1988. Ovia procurva comb. nov. is redescribed, illustrated and designated as the type species for the genus. The subfamily placement of the new genus is discussed and it is considered as a member of Lycosinae Sundevall, 1833 and possibly closely related to Alopecosa Simon, 1885. The presence of an apical process (spur) on the median apophysis is proposed as the putative synapomorphy of Ovia gen. nov. The possible sister-taxon relationship of Ovia gen. nov. with Alopecosa is discussed and evidence on the occurrence of sexual dimorphism and mating plug within the genus are presented. Ovia gen. nov. is assumed to be of Holarctic origin, from which it has migrated to the Indomalayan region. Additionally, a current distribution map for the genus is provided.

  16. A Software-Assurance Design Approach for NextGen Enabling Technologies Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Next Generation Air Transportation System (NextGen) brings significant advancements to the current management of the National Airspace (NAS). These fundamental...

  17. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    Directory of Open Access Journals (Sweden)

    Juan Carlos Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  18. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

    Directory of Open Access Journals (Sweden)

    Juan Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  19. KARAKTERISTIK SEKUEN cDNA PENGKODE GEN ANTI VIRUS DARI UDANG WINDU, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2016-11-01

    Full Text Available Transgenesis pada ikan merupakan sebuah teknik modern yang berpotensi besar dalam menghasilkan organisme yang memiliki karakter lebih baik melalui rekombinan DNA gen target termasuk gen anti virus dalam peningkatan resistensi pada udang. Gen anti virus PmAV (Penaeus monodon Anti Viral gene merupakan salah satu gen pengkode anti virus yang berasal dari spesies krustase. Penelitian ini dilakukan untuk mengetahui karakteristik gen anti virus yang diisolasi dari udang windu, Penaeus monodon. Isolasi gen anti virus menggunakan metode Polymerase Chain Reaction (PCR dan selanjutnya dipurifikasi untuk sekuensing. Data yang dihasilkan dianalisis dengan program Genetyx Versi 7 dan basic local alignment search tool (BLAST. Hasil penelitian menunjukkan bahwa gen anti virus PmAV yang berhasil diisolasi dari cDNA udang windu dengan panjang sekuen 520 bp yang mengkodekan 170 asam amino. BLAST-N menunjukkan tingkat similaritas yang sangat tinggi (100% dengan gen anti virus yang ada di GeneBank. Komposisi asam amino penyusun gen anti virus yang paling besar adalah serin (10,00%, sedangkan yang terkecil adalah asam amino prolin dan lisin masing-masing 1,76%. Analisis sekuen gen dan deduksi asam amino (BLAST-P memperlihatkan adanya C-type lectin-like domain (CTLD yang memiliki kemiripan dengan gen C-type lectin yang diisolasi dari beberapa spesies krustase. Transgenic fish technology is a potential modern technique in producing better character organism through DNA recombinant of target genes including anti viral gene for improvement of shrimp immunity. PmAV (Penaeus monodon Anti Viral gene is one of anti viral genes isolated from crustacean species. The research was conducted to analyze the characteristics anti viral gene isolated from tiger prawn, Penaeus monodon. Anti viral gene was isolated using Polymerase Chain Reaction (PCR technique and then purified for sequencing. Data obtained were analyzed using Genetyx Version 7 software and basic local alignment

  20. TRANSFER GEN ANTIVIRUS PADA EMBRIO UDANG WINDU, Penaeus monodon DALAM BERBAGAI KONSENTRASI DEOXYRIBO NUCLEIC ACID

    Directory of Open Access Journals (Sweden)

    Andi Parenrengi

    2011-12-01

    Full Text Available Teknologi transgenesis khususnya rekayasa genetik untuk menghasilkan udang windu resisten penyakit merupakan salah satu strategi yang dapat dilakukan dalam upaya pemecahan masalah penyakit yang menimpa budidaya udang windu. Teknologi transgenesis khususnya transfer gen antivirus pada udang windu telah berhasil dilakukan melalui teknik transfeksi. Meskipun demikian optimalisasi komponen teknologi tersebut masih perlu dilakukan. Konsentrasi DNA gen merupakan salah satu komponen teknologi transgenesis yang harus dioptimalkan untuk mendapatkan efisiensi dalam transfer gen. Penelitian bertujuan untuk mengetahui konsentrasi DNA gen antivirus yang optimal sebagai bahan transfer gen ke embrio menggunakan metode transfeksi. Embrio udang windu yang diperoleh dari hasil pemijahan induk asal Aceh, dikoleksi 5-10 menit setelah memijah dengan kepadatan 625 telur/2 mL. Transfeksi dilakukan dengan menggunakan media larutan transfeksi jetPEI dengan konsentrasi DNA gen antivirus sebagai perlakuan, yakni: 5, 10, dan 15 µg serta kontrol positif (tanpa plasmid DNA dan negatif (tanpa plasmid DNA dan larutan transfeksi, masing-masing 3 ulangan. Embrio hasil transfeksi ditetaskan pada stoples berisi air laut sebanyak 2 L yang diletakkan pada waterbath. Hasil penelitian menunjukkan bahwa gen antivirus telah berhasil diintroduksi ke embrio udang windu. Hasil analisis ragam menunjukkan bahwa perbedaan konsentrasi DNA (5-15 µg tidak berpengaruh nyata (P>0,05 terhadap daya tetas embrio udang windu. Analisis ekspresi gen pada larva udang windu juga menunjukkan adanya aktivitas ekspresi gen antivirus pada semua perlakuan konsentrasi DNA, di mana ekspresi gen antivirus pada larva transgenik lebih tinggi dibandingkan dengan kontrol (tanpa transfeksi. Sintasan pasca-larva PL-1 yang didapatkan pada penelitian ini adalah 12,0%; 10,0%; 10,6%; 12,3%; dan 14,2% masing-masing untuk perlakuan konsentrasi plasmid DNA 5 µg, 10 µg, 15 µg, kontrol positif dan negatif, di mana

  1. Micro-Trottoir à Genève à propos du CERN et du LHC

    CERN Multimedia

    CERN Video Productions

    2009-01-01

    Questions : Qu'est-ce-que le LHC? Quelle est la fonction du LHC? Qu'est-ce-que le CERN? D'après vous, les recherches engendrées pour le LHC peuvent-elles ?tre bénéfiques, utiles pour la société? Où a été inventé le Web? Lieux : Forum Meyrin Université de Genève Genève Centre

  2. Traffic Generator (TrafficGen) Version 1.4.2: Users Guide

    Science.gov (United States)

    2016-06-01

    2. Starting the Application 2 2.1 Prerequisites 2 2.2 Running TrafficGen 2 2.2.1 Linux 2 2.2.2 Mac OS X 2 2.2.3 Windows 2 3. Understanding the...scenarios in the examples directory. 2.2.1 Linux The following are steps to start TrafficGen on Linux . • Open a command prompt • Change directories to

  3. Stalacris Desutter-Grandcolas n. gen., an amazing cricket from South Africa (Orthoptera, Grylloidea, Phalangopsidae).

    Science.gov (United States)

    Desutter-Grandcolas, Laure

    2013-02-08

    Stalacris n. gen. (Insecta, Grylloidea, Phalangopsidae) is described from South Africa using characters of morphology and male genitalia. This taxon, known from only two species, Stalacris meridionalis n. gen., n. sp. and Stalacris sp, is characterized by the unique structure of its forewings. These are prolonged distally as long, acute and more or less articulated processes, forming a kind of forceps when forewings are opened.

  4. A 48-plex autosomal SNP GenPlex™ assay for human individualization and relationship testing

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Børsting, Claus; Morling, Niels

    2012-01-01

    SNPs are being increasingly used by forensic laboratories. Different platforms have been developed for SNP typing. We describe the GenPlex™ HID system protocol, a new SNP-typing platform developed by Applied Biosystems where 48 of the 52 SNPforID SNPs and amelogenin are included. The GenPlex™ HID...... system protocol has been successfully tested by a number of forensic laboratories using both ordinary and forensic samples....

  5. Cap a una ètica per a la genètica: fonamentació

    OpenAIRE

    Román Maestre, Begoña

    2002-01-01

    Voldria iniciar aquest article, plantejant dues qüestions bàsiques: perquè cal preguntar quina ètica per a la genètica, i perquè la genètica requereix d'una tal ètica. Pel que fa a la primera qüestió, quina ètica, cal aclarir que ha de ser una....

  6. Erections of Changa gen. nov. and Songga gen. nov. of Aolina (Hemiptera: Cicadidae: Cicadinae: Dundubiini) with taxonomic notes and a synonymization.

    Science.gov (United States)

    Lee, Young June

    2016-02-29

    Two new genera, Changa gen. nov. and Songga gen. nov., are erected in the subtribe Aolina Boulard, 2012, with the designations of Cosmopsaltria sita Distant, 1881 and Pomponia scitula Distant, 1888 as the type species, respectively. Khimbya sita (Distant, 1881) becomes Changa sita (Distant, 1881) comb. nov. Haphsa scitula (Distant, 1888) becomes Songga scitula (Distant, 1888) comb. nov. Haphsa jsguillotsi (Boulard, 2005) is transferred to Changa to become Changa jsguillotsi (Boulard, 2005) comb. nov. Haphsa stellata Lee, 2009 syn. nov. is synonymized with Changa sita. Haphsa sulaeyai (Boulard, 2005) is transferred to Macrosemia Kato, 1925 to become Macrosemia sulaeyai (Boulard, 2005) comb. nov. A revised key to the genera of Aolina is provided.

  7. Description of Teunomyces gen. nov. for the Candida kruisii clade, Suhomyces gen. nov. for the Candida tanzawaensis clade and Suhomyces kilbournensis sp. nov.

    Science.gov (United States)

    Kurtzman, Cletus P; Robnett, Christie J; Blackwell, Meredith

    2016-08-01

    DNA sequence analysis has shown that species of the Candida kruisii clade and species of the C. tanzawaensis clade represent phylogenetically circumscribed genera, which are described as Teunomyces gen. nov., type species T kruisii, and Suhomyces gen. nov., type species S tanzawaensis Many of the species are distributed worldwide and they are often isolated from fungus-feeding insects and their habitats. Included is the description of S. kilbournensis (type strain NRRL Y-17864, CBS 14276), a species found almost exclusively on maize kernels (Zea mays) in IL, USA.

  8. Saúde pública e ética na era da medicina genômica: rastreamentos genéticos

    OpenAIRE

    Bandeira,Flavia Miranda Gomes de Constantino; Gomes, Yara de Miranda; Abath,Frederico Guilherme Coutinho

    2006-01-01

    O presente artigo tem como objetivo contextualizar o campo da saúde pública diante dos grandes avanços da biotecnologia e genética aplicada, destacando elementos para a problematização do tema tais como benefícios e questões éticas relacionados aos rastreamentos genéticos. O Projeto Genoma Humano gerou várias expectativas, dentre elas, a possibilidade de rastrear genes associados a doenças e comportamentos, e mais ainda, de intervir geneticamente no ser humano, levantando preocupações relativ...

  9. Modelling of advanced structural materials for GEN IV reactors

    Science.gov (United States)

    Samaras, M.; Hoffelner, W.; Victoria, M.

    2007-09-01

    The choice of suitable materials and the assessment of long-term materials damage are key issues that need to be addressed for the safe and reliable performance of nuclear power plants. Operating conditions such as high temperatures, irradiation and a corrosive environment degrade materials properties, posing the risk of very expensive or even catastrophic plant damage. Materials scientists are faced with the scientific challenge to determine the long-term damage evolution of materials under service exposure in advanced plants. A higher confidence in life-time assessments of these materials requires an understanding of the related physical phenomena on a range of scales from the microscopic level of single defect damage effects all the way up to macroscopic effects. To overcome lengthy and expensive trial-and-error experiments, the multiscale modelling of materials behaviour is a promising tool, bringing new insights into the fundamental understanding of basic mechanisms. This paper presents the multiscale modelling methodology which is taking root internationally to address the issues of advanced structural materials for Gen IV reactors.

  10. Pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce.

    Science.gov (United States)

    Tanasupawat, Somboon; Thongsanit, Jaruwan; Thawai, Chitti; Lee, Keun Chul; Lee, Jung-Sook

    2011-07-01

    Two strains of Gram-stain-positive, catalase-negative, tetrad-forming cocci, C01(T) and C02, were isolated in Thailand from fish sauce. They were facultatively anaerobic, non-motile and non-spore-forming bacteria. These strains produced l-lactic acid from glucose. They grew at pH 5.0-9.0, at 15-40 °C and in the presence of 10 % (w/v) NaCl. The dominant fatty acid was C(18 : 1)ω9c. The DNA G+C contents of strains C01(T) and C02 were 38.6 and 38.7 mol%, respectively. Strain C01(T) was related most closely to Desemzia incerta DSM 20581(T), with a 16S rRNA gene sequence similarity of 96.9 %. The strains could be distinguished clearly from D. incerta DSM 20581(T) based on cell morphology, physiological and biochemical characteristics and low levels of DNA-DNA relatedness. On the basis of the data presented, strains C01(T) and C02 are considered to represent a novel species of a new genus in the Bacillus-Lactobacillus cluster, for which the name Pisciglobus halotolerans gen. nov., sp. nov. is proposed. The type strain of Pisciglobus halotolerans is C01(T) ( = KCTC 13150(T)  = TISTR 1958(T)  = PCU 316(T)).

  11. De compras por el supermercado genético

    Directory of Open Access Journals (Sweden)

    Singer, Peter

    2002-12-01

    Full Text Available Should we say that selective abortion is a “powerful message that we seek to eliminate future persons”? When deaf parents want to have a deaf children, Are they crazy? Are they advocates of a culture –the Deaf culture? If genetic engineering can give us health, intelligent, and athletic childrens, why say not to this advantages? The aim of this article is not to deal with all objections that could be urged against these options; the purpose is developing a clear understanding of the central values at stake.

    ¿Estamos eliminando una cultura, la de los sordos, cuando tratamos de evitar que nazcan niños sordos? El aborto terapéutico, ¿significa que, por ejemplo, creemos que las vidas de los afectados por síndrome de Down son vidas de menor valor que las vidas “normales”? Si se permitiera la manipulación genética de los embriones ¿sería poco ético encargar hijos guapos y altos? Este artículo no aporta respuestas a estas preguntas, pero sí que plantea los términos para dar cuenta de ellas y eleva acta de lo difícil que es dar una respuesta concluyente.

  12. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier d...

  13. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet - 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier ...

  14. Testes genéticos na eqüideocultura

    Directory of Open Access Journals (Sweden)

    Eduardo Geraldo Alves Coelho

    2008-07-01

    Full Text Available Nos últimos anos a equideocultura deu um salto qualitativo, havendo hoje, no mercado, animais de alto valor e geneticamente superiores. Isso é possível, em grande parte, devido aos avanços na área da genética animal, os quais permitem identificar, não apenas anomalias, mas também diversos genes de interesse econômico. Com o auxílio da citogenética pode-se identificar indivíduos com alterações no número ou na estrutura dos cromossomos, o que em muitos casos afeta principalmente a reprodução. Também a confirmação de genealogia, anteriormente feita por tipagem sangüínea e atualmente por testes de DNA, tem papel extremamente importante, não apenas por garantir a ascendência dos animais, mas também porque um pedigree confiável pode permitir ao criador identificar a origem de problemas genéticos em seu rebanho e reduzi-los ou mesmo, eliminá-los. Ainda com as ferramentas da biologia molecular, podemos hoje, identificar indivíduos que apresentam genes desejáveis ou indesejáveis, o que nos permite selecioná-los precocemente, reduzindo assim, os custos do produtor e aumentando o valor agregado dos animais. Entre tais genes podemos destacar os que identificam portadores ou afetados por mutações genéticas indesejáveis como: SCID (Síndrome da Imunodeficiência Combinada, HYPP (Parilisa Hipercalêmica, HERDA (Astenia Dérmica Regional Hereditária Eqüina, etc. Também a identificação dos genes que determinam a cor ou padrão da pelagem já pode ser feita direta ou indiretamente (por meio de marcadores genéticos, como é o caso dos genes para as pelagens Overo, Tobiano, etc. Com os avanços no estudo do genoma eqüino muito mais estará disponível em breve, o que certamente só trará maiores contribuições à equideocultura mundial.In the last few years the horse breeding industry is achieving significant progresses producing animals of high commercial value and genetically superior. It was possible, mainly due to

  15. GenFamClust: an accurate, synteny-aware and reliable homology inference algorithm.

    Science.gov (United States)

    Ali, Raja H; Muhammad, Sayyed A; Arvestad, Lars

    2016-06-04

    Homology inference is pivotal to evolutionary biology and is primarily based on significant sequence similarity, which, in general, is a good indicator of homology. Algorithms have also been designed to utilize conservation in gene order as an indication of homologous regions. We have developed GenFamClust, a method based on quantification of both gene order conservation and sequence similarity. In this study, we validate GenFamClust by comparing it to well known homology inference algorithms on a synthetic dataset. We applied several popular clustering algorithms on homologs inferred by GenFamClust and other algorithms on a metazoan dataset and studied the outcomes. Accuracy, similarity, dependence, and other characteristics were investigated for gene families yielded by the clustering algorithms. GenFamClust was also applied to genes from a set of complete fungal genomes and gene families were inferred using clustering. The resulting gene families were compared with a manually curated gold standard of pillars from the Yeast Gene Order Browser. We found that the gene-order component of GenFamClust is simple, yet biologically realistic, and captures local synteny information for homologs. The study shows that GenFamClust is a more accurate, informed, and comprehensive pipeline to infer homologs and gene families than other commonly used homology and gene-family inference methods.

  16. Diaminobutyricibacter tongyongensis gen. nov., sp. nov. and Homoserinibacter gongjuensis gen. nov., sp. nov. belong to the family Microbacteriaceae.

    Science.gov (United States)

    Kim, Soo-Jin; Ahn, Jae-Hyung; Weon, Hang-Yeon; Hamada, Moriyuki; Suzuki, Ken-ichiro; Kwon, Soon-Wo

    2014-06-01

    Two bacterial strains, KIS66-7(T) and 5GH26-15(T), were isolated from soil samples collected in the South Korean cities of Tongyong and Gongju, respectively. Both strains were aerobic, Gram-stain-positive, mesophilic, flagellated, and rodshaped. A phylogenetic analysis revealed that both strains belonged to the family Microbacteriaceae of the phylum Actinobacteria. The 16S rRNA gene sequence of strain KIS66-7(T) had the highest similarities with those of Labedella gwakjiensis KSW2-17(T) (97.3%), Cryobacterium psychrophilum DSM 4854T (97.2%), Leifsonia lichenia 2Sb(T) (97.2%), Leifsonia naganoensis JCM 10592(T) (97.0%), and Cryobacterium mesophilum MSL-15(T) (97.0%). Strain 5GH26-15(T) showed the highest sequence similarities with Leifsonia psychrotolerans LI1T (97.4%) and Schumannella luteola KHIAT (97.1%). The 16S rRNA gene sequence from KIS66-7(T) exhibited 96.4% similarity with that from 5GH26-15(T). Strain KIS66-7(T) contained a B2γ type peptidoglycan structure with D-DAB as the diamino acid; MK-13, MK-12, and MK-14 as the respiratory quinones; ai-C15:0, ai-C17:0, and i-C16:0 as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Strain 5GH26-15T had a B2β type peptidoglycan structure with D-DAB as the diamino acid; MK-14 and MK-13 as the respiratory quinones; ai-C15:0, i-C16:0, and ai-C{vn17:0} as the major cellular fatty acids; and diphosphatidylglycerol, phatidylglycerol, and glycolipids as the predominant polar lipids. Both strains had low DNA-DNA hybridization values (gen. nov., sp. nov. (type strain KIS66-7(T)=KACC 15515(T)=NBRC 108724(T)) and Homoserinibacter gongjuensis gen. nov., sp. nov. (type strain 5GH26-15(T)=KACC 15524(T)=NBRC 108755(T)) within the family Microbacteriaceae.

  17. Genética molecular de las hemoglobinas bacterianas: estructura, regulación y función

    OpenAIRE

    Elizabeth Hernández Urzúa; Jorge Membrillo Hernández

    2004-01-01

    Las hemoglobinas se definen como aquellas hemoproteínas que unen reversiblemente el oxígeno. Estas proteínas se encuentran distribuidas en plantas, protozoarios, hongos y bacterias. Las hemoglobinas presentes en los microorganismos se han dividido en tres grupos. En el primer grupo se encuentran aquellas hemoproteínas que presentan un solo dominio con el grupo hemo, por ejemplo la hemoglobina de Vitreoscilla. En el segundo se encuentran aquellas hemoglobinas que presentan dos diferentes domin...

  18. Enfermedades genéticas más frecuentes en pacientes atendidos en consulta de genética clínica

    Directory of Open Access Journals (Sweden)

    Elibett Carcasés Carcasés

    2015-02-01

    Full Text Available La estimación de la prevalencia de las enfermedades genéticas se dificulta, entre otras causas, por su rareza. Se realizó un estudio descriptivo retrospectivo, para identificar las enfermedades genéticas de mayor prevalencia en pacientes atendidos por este programa en el Centro Provincial de Genética Médica de Las Tunas, Cuba; desde el año 1989 hasta julio de 2014. Se revisaron todas las historias clínicas. Predominó el origen monogénico (69 %, siendo los síndromes dismórficos los más numerosos y diversos, entre ellos los neurocutáneos, que representaron el 35 %. La enfermedad genética monogénica con mayor número de casos fue la Neurofibromatosis I con el 14,4 % y el 22,2 % de las enfermedades eran de origen monogénico y dismórfico. La Trisomía 21 representó el 77 % de la causa cromosómica. En el origen multifactorial prevalecieron los defectos congénitos mayores, entre ellos los defectos reductivos de miembros (27 %

  19. Critical Issues on Materials for Gen-IV Reactors

    Energy Technology Data Exchange (ETDEWEB)

    Caro, M; Marian, J; Martinez, E; Erhart, P

    2009-02-27

    Within the LDRD on 'Critical Issues on Materials for Gen-IV Reactors' basic thermodynamics of the Fe-Cr alloy and accurate atomistic modeling were used to help develop the capability to predict hardening, swelling and embrittlement using the paradigm of Multiscale Materials Modeling. Approaches at atomistic and mesoscale levels were linked to build-up the first steps in an integrated modeling platform that seeks to relate in a near-term effort dislocation dynamics to polycrystal plasticity. The requirements originated in the reactor systems under consideration today for future sources of nuclear energy. These requirements are beyond the present day performance of nuclear materials and calls for the development of new, high temperature, radiation resistant materials. Fe-Cr alloys with 9-12% Cr content are the base matrix of advanced ferritic/martensitic (FM) steels envisaged as fuel cladding and structural components of Gen-IV reactors. Predictive tools are needed to calculate structural and mechanical properties of these steels. This project represents a contribution in that direction. The synergy between the continuous progress of parallel computing and the spectacular advances in the theoretical framework that describes materials have lead to a significant advance in our comprehension of materials properties and their mechanical behavior. We took this progress to our advantage and within this LDRD were able to provide a detailed physical understanding of iron-chromium alloys microstructural behavior. By combining ab-initio simulations, many-body interatomic potential development, and mesoscale dislocation dynamics we were able to describe their microstructure evolution. For the first time in the case of Fe-Cr alloys, atomistic and mesoscale were merged and the first steps taken towards incorporating ordering and precipitation effects into dislocation dynamics (DD) simulations. Molecular dynamics (MD) studies of the transport of self

  20. Electronic and magnetic properties of CrGen (15 ⩽ n ⩽ 29) clusters: A DFT study

    Science.gov (United States)

    Mahtout, Sofiane; Tariket, Yacine

    2016-06-01

    We report ab initio calculations of electronic and magnetic properties of medium-sized CrGen (15 ⩽ n ⩽ 29) clusters using density functional theory. The encapsulation of Cr atoms within Gen clusters leads to stable Cr encapsulated Gen clusters. The binding energies generally increase while the differences between the highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO-LUMO gaps) generally decrease with the increasing of cluster size. The clusters of CrGen at size 16, 17, 19, 22, 24 and 29 exhibit high stabilities when compared to their neighbors. This has been discussed in terms of their structures, energies and the effect of the position of doping atom. Doping of Gen clusters with one Cr atom leads to CrGen clusters with magnetic moment depending on the structure of the clusters and the position of Cr atom in the clusters. Moreover, vertical ionization potential, vertical electronic affinity, and chemical hardness are also analyzed.

  1. Mejoramiento genético vegetal in vitro

    Directory of Open Access Journals (Sweden)

    Antonia Gutiérrez M.

    2003-01-01

    Full Text Available El mejoramiento genético de plantas es una de las hazañas más antiguas del hombre, que inició con la domesticación de las mismas bajo condiciones controladas y la selección de aquellas capaces de proporcionar una mejor fuente de alimentos. Esto marcó una de las fases más importantes en el progreso de la humanidad, al permitirle transitar de una vida nómada e individualista a una sociedad organizada y cooperativista. Dicho mejoramiento fue fortuito y lento y permaneció como un arte y no como una ciencia hasta principios del siglo XX, luego de que las llamadas leyes de Mendel, pioneras en la explicación de los procesos de la herencia, obtuvieron reconocimiento (Briggs y Knowles, 1967. El proceso que emplea fitomejoradores ha creado un sinnúmero de variedades de plantas con el objeto de incrementar su producción, resistencia a plagas y enfermedades, y la adaptación a ambientes específicos, regiones y usos, mediante la selección de variedades cultivadas localmente, cruzadas entre sí o con las de otras áreas, o también con plantas silvestres que tengan los genes deseados. Sin embargo, obtener plantas mejoradas por estos medios resulta difícil en ocasiones por lo que se recurre a otros métodos para producir variantes útiles, tales como la selección celular, la variación somaclonal y las mutaciones inducidas, entre otros

  2. Haloactinomyces albus gen. nov., sp. nov., isolated from Dead Sea.

    Science.gov (United States)

    Lai, Hangxian; Jiang, Yingying; Chen, Xiu; Li, Qinyuan; Jiang, Chenglin; Jiang, Yi; Wei, Xiaomin

    2017-01-10

    A novel halophilic, filamentous actinomycete strain, designated AFM 10258T, was isolated from a sediment sample collected from Dead Sea of Israel. The isolate grew with 10-35% NaCl and did not grow without NaCl. The isolate formed white aerial mycelium and long spore chains, and two spores were separated by sterile mycelium. The spores were non-motile, spherical and rugose-surfaced. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. Major fatty acids were anteiso-C17:0, iso-C16:0 and iso-C15:0. MK-9(H4) was the predominant menaquinone and the DNA G+C content was 62.8 mol%. 16S rRNA gene sequence analysis indicated that strain AFM 10258T shared low sequence similarity with the closely related representatives of the families Pseudonocardiaceae (below 94.47%) and Actinopolysporaceae (below 93.76%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AFM 10258T formed a robust clade with members of the family Actinopolysporaceae. On the basis of analysis of phenotypic, chemical and molecular characteristics, strain AFM 10258T is considered to represent a novel species of a new genus , for which the name Haloactinomyces albus gen. nov., sp. nov., is proposed. The type strain is AFM 10258T (=DSM 45977T = CGMCC 4.7115T).

  3. Exploración de la variabilidad genética del maracuyá (Passiflora edulis f. flavicarpa Degener como base para un programa de fitomejoramiento en Colombia

    Directory of Open Access Journals (Sweden)

    John Ocampo

    2013-12-01

    Full Text Available El maracuyá es uno de los principales frutales del Neotrópico y en Colombia existen cerca de 6000 ha cultivadas con esta fruta, con una producción de 90,000 t/año. No obstante este potencial, los cultivos presentan graves problemas fitosanitarios y degeneración genética, lo que se manifiesta por una reducción de la vida útil de la plantación. El objetivo de esta investigación fue evaluar el grado de variabilidad genética del maracuyá cultivado en Colombia como base para un programa de fitomejoramiento. Las recolecciones se realizaron en los departamentos de Antioquia, Caldas, Cauca, Huila, Tolima y Valle del Cauca mediante una selección masal participativa con los productores. En cada cultivo, se seleccionaron diez frutos al azar (calidad extra de las plantas más sobresalientes por productividad y sanidad, en las cuales se registró la incidencia de insectos plaga y enfermedades. Los frutos fueron caracterizados con 11 variables fisicoquímicas y analizados mediante la descomposición de la varianza (univariado y el análisis de clasificación (neighbour joining. Los resultados mostraron que los trips (Neohydatothrips spp. y la virosis (SMV son los problemas fitosanitarios que más afectan el cultivo en los sitios del estudio. El análisis univariado del fruto mostró un porcentaje de variabilidad promedio total de 14.31% (CV, destacándose los pesos de la cáscara (20.53% y de la semilla (20.47%. Tomando en cuenta los parámetros de calidad (°Brix y %pulpa + semilla se identificaron ocho accesiones élite provenientes de Caldas, Valle del Cauca y Antioquia. El análisis de clasificación mostró una alta variabilidad, con poca estructuración por origen geográfico. Estos resultados permitirán iniciar un proceso de mejoramiento genético a partir de genotipos superiores de las accesiones élite identificadas.

  4. Genética em transtornos alimentares: ampliando os horizontes de pesquisa

    Directory of Open Access Journals (Sweden)

    Pinheiro Andréa Poyastro

    2006-01-01

    Full Text Available OBJETIVO: Revisar a literatura atual concernente à pesquisa genética em transtornos do comportamento alimentar e discutir questões relevantes ao desenvolvimento de um projeto de pesquisa genética nessa área no Brasil. MÉTODO: A revisão realizada utilizou a base de dados Medline, no período de 1984 a maio de 2005, com os seguintes termos de busca: "anorexia nervosa", "bulimia nervosa", "eating disorders", "binge eating disorder", "family studies", "twin studies", "molecular genetics studies". RESULTADOS: Os dados atuais apontam para uma contribuição relevante dos fatores genéticos na suscetibilidade à anorexia e à bulimia nervosa. A pesquisa genética com populações miscigenadas deve levar em consideração o tamanho da amostra, a densidade de genotipagem e a estratificação populacional. Através de "admixture mapping" é possível estimar a estrutura genética destas populações e localizar genes relacionados à variação étnica de doenças ou traços de interesse. CONCLUSÕES: O desenvolvimento de uma grande iniciativa de colaboração em genética de transtornos alimentares no Brasil e na América Latina viabilizará estudar os fatores genéticos em transtornos do comportamento alimentar no contexto de grupos inter-étnicos, e integrar uma nova perspectiva biológica à etiologia destes distúrbios.

  5. Salud pública, genética y ética

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    Kottow Miguel H

    2002-01-01

    Full Text Available La investigación genética ha tenido una enorme expansión en recientes décadas, con repercusiones terapéuticas aún inciertas. El análisis bioético tradicional de las complejas prácticas genéticas ha sido insuficiente por sostenerse en la ética de la investigación y en la bioética de corte principialista. Los problemas éticos más importantes de la genética son de orden colectivo y deben ser abordados por una reflexión ético-social cuyo enfoque es más amplio que la agenda interpersonal del principialismo. Temas como exploraciones genéticas, cuestiones patrimoniales, manipulación génica y asignación de recursos, deben todos ser sometidos a un pensamiento inspirado en los requerimientos de la ciudadanía, en el bien común y en la definición del rol del Estado en fiscalizar actividades genéticas y en proteger a la población. El objetivo del estudio es mostrar cómo el amplio campo de la ética y de la genética tiene una mayor relevancia en el campo social que en el clínico. El objetivo del trabajo es señalar que la bioética principialista ha enfatizado los problemas éticos individuales que nacen con la intervención genética, a costa de marginar sus importantes repercusiones sociales.

  6. Salud pública, genética y ética

    Directory of Open Access Journals (Sweden)

    Miguel H Kottow

    2002-10-01

    Full Text Available La investigación genética ha tenido una enorme expansión en recientes décadas, con repercusiones terapéuticas aún inciertas. El análisis bioético tradicional de las complejas prácticas genéticas ha sido insuficiente por sostenerse en la ética de la investigación y en la bioética de corte principialista. Los problemas éticos más importantes de la genética son de orden colectivo y deben ser abordados por una reflexión ético-social cuyo enfoque es más amplio que la agenda interpersonal del principialismo. Temas como exploraciones genéticas, cuestiones patrimoniales, manipulación génica y asignación de recursos, deben todos ser sometidos a un pensamiento inspirado en los requerimientos de la ciudadanía, en el bien común y en la definición del rol del Estado en fiscalizar actividades genéticas y en proteger a la población. El objetivo del estudio es mostrar cómo el amplio campo de la ética y de la genética tiene una mayor relevancia en el campo social que en el clínico. El objetivo del trabajo es señalar que la bioética principialista ha enfatizado los problemas éticos individuales que nacen con la intervención genética, a costa de marginar sus importantes repercusiones sociales.

  7. Wave Data Processing and Analysis, Part 2: Codes for Coupling GenCade and CMS-Wave

    Science.gov (United States)

    2013-09-01

    coastal modeling system , Report 2: CMS -Wave. ERDC/CHL-TR-11-10. Vicksburg, MS: US Army Engineer Research and Development Center. Connell, K. J. and...Coupling GenCade and CMS -Wave by Rusty Permenter, Kenneth J. Connell, and Zeki Demirbilek PURPOSE: This Coastal and Hydraulics Engineering...to GenCade. This is the second CHETN in a two‐part series detailing the process of coupling CMS ‐Wave with GenCade. This CHETN focuses on

  8. Efecto de las variantes genéticas sobre los parámetros enzimáticos de la butirilcolinesterasa humana

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    Jennifer Castro

    2016-06-01

    Full Text Available Introducción: Las enzimas colinesterasas humanas – acetilcolinesterasa (AChE y la butirilcolinesterasa (BChE – se presentan a lo largo de la mayoría de los principales sistemas de órganos en una variedad de seres vivos. La BChE es una alfa glicoproteína que hidroliza ésteres de colina, compuestos alifáticos y algunos fármacos. Esta enzima presenta gran cantidad de variantes genéticas – más de 100 variantes han sido identificadas, entre las más estudiadas se encuentra la variante Kalow (K, la cual es la más frecuentemente encontrada en varias poblaciones humanas. La actividad de la BChE se puede medir por diferentes métodos: colorimétricos, potenciométricos, entre otros. Uno de los  métodos más sencillos y económicos para medir la actividad colinesterasa es el potenciométrico de Michel. Este estudio busca identificar el efecto del polimorfismo rs1803274 (variante-K en los parámetros enzimáticos de la butirilcolinesterasa humana mediante el método potenciométrico de Michel. Objetivo: Determinar si la presencia del polimorfismo genético rs1803274 (BChE modifica los parámetros enzimáticos Vmax y Km de la butirilcolinesterasa humana. Materiales y Métodos: Se utilizaros 9 muestras de sangre de personas portadoras de la variante K (homocigotos y heterocigotos. Las muestras de sangre fueron colectadas en tubos de heparina. Para las determinaciones enzimáticas se empleó el método potenciométrico de Michel y se variaron las condiciones de reacción (pH, temperatura, concentración de sustrato y efecto de un inhibidor. Resultados: Se encontró una actividad enzimática menor cuando la variante-K estaba presente en la BChE en comparación con la enzima no polimórfica. Esta diferencia iba en aumento a medida que se incrementaba la temperatura del medio. Conclusiones: La disminución de la actividad enzimática portadora de la variante-K se correlacionó con los cambios de temperatura. Esto sugiere que la disminución de

  9. Medicina genómica: Aplicaciones del polimorfismo de un nucleótido y micromatrices de ADN Genomic Medicine: Polymorphisms and microarray applications

    Directory of Open Access Journals (Sweden)

    Monica P. Spalvieri

    2004-12-01

    Full Text Available Esta actualización tiene por objeto difundir un nuevo enfoque de las variaciones del ADN entre individuos y comentar las nuevas tecnologías para su detección. La secuenciación total del genoma humano es el comienzo para conocer la diversidad genética. La unidad de medida reconocida de esta variabilidad es el polimorfismo de un solo nucleótido (single nucleotide polymorphism o SNP. El estudio de los SNPs está restringido a la investigación pero las numerosas publicaciones sobre el tema hacen vislumbrar su entrada en la práctica clínica. Se presentan ejemplos del uso de SNPs como marcadores moleculares en la genotipificación étnica, la expresión génica de enfermedades y como potenciales blancos farmacológicos. Se comenta la técnica de las matrices (arrays que facilita el estudio de múltiples secuencias de genes mediante chips de diseño específico. Los métodos convencionales analizan hasta un máximo de 20 genes, mientras que una sola micromatriz provee información sobre decenas de miles de genes simultáneamente con una genotipificación rápida y exacta. Los avances de la biotecnología permitirán conocer, además de la secuencia de cada gen, la frecuencia y ubicación exacta de los SNPs y su influencia en los comportamientos celulares. Si bien la validez de los resultados y la eficiencia de las micromatrices son aún controvertidos, el conocimiento y caracterización del perfil genético de un paciente impulsará seguramente un cambio radical en la prevención, diagnóstico, pronóstico y tratamiento de las enfermedades humanas.This update shows new concepts related to the significance of DNA variations among individuals, as well as to their detection by using a new technology. The sequencing of the human genome is only the beginning of what will enable us to understand genetic diversity. The unit of DNA variability is the polymorphism of a single nucleotide (SNP. At present, studies on SNPs are restricted to basic research

  10. Papel de ginA y ginB en la movilidad de la isla genómica GInt

    OpenAIRE

    Echeverría Ancín, Myriam

    2014-01-01

    La transferencia horizontal de genes, mediada por elementos genéticos móviles tales como las islas genómicas, es uno de los principales mecanismos de generación de variabilidad genética en bacterias, permitiendo su adaptación a nuevos nichos ecológicos. En el grupo de Patología Vegetal de la UPNA se ha descrito, en especies de Pseudomonas, una nueva familia de islas genómicas, denominadas GInt. Éstas se caracterizan por contener en su extremo 5’ el operón gin, constituido ...

  11. La Dislexia del Desarrollo: Gen, Cerebro y Cognición Developmental Dyslexia: Gen, Brain, and Cognition

    Directory of Open Access Journals (Sweden)

    Alberto M Galaburda

    2006-11-01

    Full Text Available La dislexia del desarrollo es un trastorno que se caracteriza por dificultades en el aprendizaje de la lectura. Recientemente se ha podido vincular la dislexia a cuatro distintos genes candidatos de riesgo: DYX1C1, KIAA0319, DCDC2 y ROBO1. Estos cuatro genes participan en el desarrollo cerebral, y anomalías de dicho desarrollo constituyen los elementos conocidos del cuadro biológico que subyace a la dislexia. En animales experimentales, la inducción de anomalías del desarrollo cerebral similares produce problemas en el procesamiento de ciertos sonidos. En humanos, problemas de procesamiento de sonidos semejantes se asocian a un trastorno de aprendizaje de la lectura. Por consiguiente, es posible por primera vez, trazar una trayectoria tentativa entre una característica genética, variaciones del desarrollo del cerebro, y trastornos conductuales y cognitivos asociados a la dislexia.Developmental dyslexia is a disorder characterized by difficulties in reading acquisition. Recently, dyslexia has been related to four different genes which are prone-risk candidates: DYX1C1, KIAA0319, DCDC2, and ROBO1. These four genes participate in brain development, and anomalies in that development comprise the known elements of the biological constellation underlying dyslexia. The induction of similar brain development anomalies in experimental animals produces problems in the processing of certain sounds. In humans, similar sound processing problems are related to a reading acquisition disorder. Consequently, for the first time it is possible to delineate a tentative path between a genetic characteristic, brain development variations, and behavioral and cognitive disorders related to dyslexia.

  12. Biometria e armazenamento de sementes de genótipos de cacaueiro

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    Lucimara Ribeiro Venial

    2017-03-01

    Full Text Available Genótipos de Theobroma cacao L. devem ser melhor estudados, para se identificar aqueles que produzem sementes mais desenvolvidas e viáveis após o armazenamento. Objetivou-se com este trabalho estudar a biometria e dois tempos de armazenamento de sementes de genótipos de cacaueiro. A biometria foi avaliada em oito genótipos de cacaueiro (tratamentos. Foram instalados testes de germinação em delineamento inteiramente ao acaso, no esquema fatorial 8 x 2 (genótipos: CCN51, PH16, CEPEC2002, Ipiranga, SJ02, PS1319, TSH1188 e Comum x dois períodos de armazenamento: 0 e dois dias. O genótipo TSH1188 apresentou maior comprimento, relação comprimento/largura, espessura e massa de 100 sementes. A absorção de água das sementes recém-colhidas dos genótipos é lenta, justificada pelos altos teores de água, o que não caracteriza padrão-trifásico. Os teores de água reduziram em média 2,3 vezes nas sementes armazenadas em relação às recém-colhidas. A germinação das sementes recém-colhidas dos genótipos foi de 100%. Após o armazenamento, as sementes do PS1319 apresentaram a menor redução da germinação (39%, enquanto as dos PH16, CEPEC2002 e SJ02 reduziram 96%. A velocidade de germinação foi maior e o tempo médio menor que dois dias nas sementes recém-colhidas do PS1319, indicando serem mais tolerante à dessecação. Sugere-se o uso dos genótipos TSH1188 e PS1319 em programas de melhoramento genético.

  13. ATLAS Versus NextGen Model Atmospheres: A Combined Analysis of Synthetic Spectral Energy Distributions

    Science.gov (United States)

    Bertone, E.; Buzzoni, A.; Chávez, M.; Rodríguez-Merino, L. H.

    2004-08-01

    We carried out a critical appraisal of the two theoretical models, Kurucz' ATLAS9 and PHOENIX/NextGen, for stellar atmosphere synthesis. Our tests relied on the theoretical fit of spectral energy distributions (SEDs) for a sample of 334 target stars along the whole spectral-type sequence, from the classical optical catalogs of Gunn & Stryker and Jacoby et al. The best-fitting physical parameters (Teff, logg) of stars allowed an independent calibration of the temperature and bolometric scale versus empirical classification parameters (i.e., spectral type and MK luminosity class); in addition, the comparison of the synthetic templates from the ATLAS and NextGen grids allowed us to probe the capability of the models to match spectrophotometric properties of real stars and assess the impact of the different input physics. We can sketch the following main conclusions of our analysis: (1) Fitting accuracy of both theoretical libraries drastically degrades at low Teff at which both ATLAS and NextGen models still fail to properly account for the contribution of molecular features in the observed SED of K-M stars. (2) Compared with empirical calibrations, both ATLAS and NextGen fits tend, on average, to predict slightly warmer (by 4%-8%) Teff for both giant and dwarf stars of fixed spectral type, but ATLAS provides, in general, a sensibly better fit (a factor of 2 lower σ of flux residuals) than NextGen. (3) There is a striking tendency of NextGen to label target stars with an effective temperature and surface gravity higher than that of ATLAS. The effect is especially evident for MK I-III objects for which about one in four stars is clearly misclassified by NextGen in logg. This is a consequence of some ``degeneracy'' in the solution space, partly induced by the different input physics and geometry constraints in the computation of the integrated emerging flux (ATLAS model atmospheres assume standard plane-parallel layers, while NextGen adopts, for low-gravity stars, a

  14. Variabilidad genética y gestión forestal

    Directory of Open Access Journals (Sweden)

    R. Alía

    2003-01-01

    Full Text Available La diversidad biológica, su conservación e incremento, es uno de los principios básicos de la gestión forestal sostenible. La alta variabilidad genética de las especies forestales es responsable de los procesos de adaptación ante factores bióticos y abióticos extremos que, a su vez, aseguran la persistencia frente a los riesgos a los que están sometidas las masas forestales. En este trabajo se analizan distintos aspectos relacionados con la variabilidad genética de las especies forestales, y como la gestión forestal influye sobre ella. Se analiza la variabilidad entre poblaciones (que es una de las bases de la comercialización de material forestal de reproducción, la influencia del sistema de reproducción en la variabilidad genética de las masas y los efectos en ellas de los tratamientos selvícolas, así como la variación individual y clonal aprovechada en los programas de selección. Por último, se analiza el papel de la variabilidad genética en los programas de mejora y conservación de recursos genéticos forestales

  15. ProGenExpress: Visualization of quantitative data on prokaryotic genomes

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    Watson Michael

    2005-04-01

    Full Text Available Abstract Background The integration of genomic information with quantitative experimental data is a key component of systems biology. An increasing number of microbial genomes are being sequenced, leading to an increasing amount of data from post-genomics technologies. The genomes of prokaryotes contain many structures of interest, such as operons, pathogenicity islands and prophage sequences, whose behaviour is of interest during infection and disease. There is a need for simple and novel tools to display and analyse data from these integrated datasets, and we have developed ProGenExpress as a tool for visualising arbitrarily complex numerical data in the context of prokaryotic genomes. Results Here we describe ProGenExpress, an R package that allows researchers to easily and quickly visualize quantitative measurements, such as those produced by microarray experiments, in the context of the genome organization of sequenced prokaryotes. Data from microarrays, proteomics or other whole-genome technologies can be accurately displayed on the genome. ProGenExpress can also search for novel regions of interest that consist of groups of adjacent genes that show similar patterns across the experimental data set. We demonstrate ProGenExpress with microarray data from a time-course experiment involving Salmonella typhimurium. Conclusion ProGenExpress can be used to visualize quantitative data from complex experiments in the context of the genome of sequenced prokaryotes, and to find novel regions of interest.

  16. PERAN GEN AIM1 DAN INTENSITAS CAHAYA TERHADAP KARAKTER POLA PIGMEN IKAN BADUT HITAM (Amphiprion percula

    Directory of Open Access Journals (Sweden)

    Daniar Kusumawati

    2012-08-01

    Full Text Available Pola pigmen merupakan faktor utama yang menentukan tingginya kualitas ikan hias. Pada benih-benih F1 populasi budidaya ikan badut hitam (Amphiprion percula diketahui mengalami degeneratif pola pigmen yang menyimpang dibandingkan dengan populasi di alam. Pola pigmen merupakan salah satu karakter fenotip yang diturunkan di mana ekspresinya bergantung pada interaksi genetik dengan faktor eksternal yaitu lingkungan. Tujuan dari penelitian ini adalah untuk mengkaji peran gen yaitu Aim1 yang mengontrol sintesis melanin dan kondisi lingkungan dalam menginduksi pola pigmen hitam ikan badut hitam (Amphiprion percula. Analisis gen Aim1 dilakukan dengan menggunakan program speedy PCR dilanjutkan dengan SSCP (single strand confirmation polymorphism untuk mengidentifikasi variasi genetik dari gen Aim1. Analisis SDS Page dilakukan untuk mengetahui peranan lingkungan terhadap profil protein yang disintesis. Berdasarkan hasil penelitian diketahui bahwa primer Aim1 memiliki sisi pengenalan pada whole genom ikan badut hitam (Amphiprion percula pada target sequence 45 bp. Berdasarkan analisis SSCP profil fragmentasi amplicon primer Aim1 pada masing-masing tingkatan pada populasi budidaya homolog dengan populasi alam, sehingga gen Aim1 bukan merupakan gen yang mengontrol fenomena degeneratif pola pigmen ikan badut hitam (Amphiprion percula. Peran lingkungan yaitu cahaya memberikan pengaruh positif dalam menginduksi pola pigmen melalui stimulus pada sistem neuron dan migrasi melanophore.

  17. Diversidade genética molecular de progênies de dendezeiro

    Directory of Open Access Journals (Sweden)

    Crystianne Bentes Barbosa Ferreira

    2012-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a diversidade genética, entre e dentro de progênies de dendezeiro tipo dura, de origem Deli. A caracterização genética foi feita com uso de marcadores microssatélites em 24 progênies usadas na produção comercial de sementes, sendo 22 provenientes de autofecundação e duas de cruzamentos entre irmãos completos. Foi realizada análise de variância molecular entre e dentro das progênies, com posterior construção de um dendrograma. Observou-se baixa variabilidade genética nas progênies, com média de 1,32 alelos por loco e variância genética total igual a 0,3241. A maior parte da variação ocorreu entre progênies. A menor variabilidade genética dentro das progênies pode ser explorada nos cruzamentos com progênies endogâmicas de outras origens, o que facilitaria o alcance de heterose para o desenvolvimento de novas variedades.

  18. Human Holliday junction resolvase GEN1 uses a chromodomain for efficient DNA recognition and cleavage.

    Science.gov (United States)

    Lee, Shun-Hsiao; Princz, Lissa Nicola; Klügel, Maren Felizitas; Habermann, Bianca; Pfander, Boris; Biertümpfel, Christian

    2015-12-18

    Holliday junctions (HJs) are key DNA intermediates in homologous recombination. They link homologous DNA strands and have to be faithfully removed for proper DNA segregation and genome integrity. Here, we present the crystal structure of human HJ resolvase GEN1 complexed with DNA at 3.0 Å resolution. The GEN1 core is similar to other Rad2/XPG nucleases. However, unlike other members of the superfamily, GEN1 contains a chromodomain as an additional DNA interaction site. Chromodomains are known for their chromatin-targeting function in chromatin remodelers and histone(de)acetylases but they have not previously been found in nucleases. The GEN1 chromodomain directly contacts DNA and its truncation severely hampers GEN1's catalytic activity. Structure-guided mutations in vitro and in vivo in yeast validated our mechanistic findings. Our study provides the missing structure in the Rad2/XPG family and insights how a well-conserved nuclease core acquires versatility in recognizing diverse substrates for DNA repair and maintenance.

  19. Mechanism of Holliday junction resolution by the human GEN1 protein.

    Science.gov (United States)

    Rass, Ulrich; Compton, Sarah A; Matos, Joao; Singleton, Martin R; Ip, Stephen C Y; Blanco, Miguel G; Griffith, Jack D; West, Stephen C

    2010-07-15

    Holliday junction (HJ) resolution is essential for chromosome segregation at meiosis and the repair of stalled/collapsed replication forks in mitotic cells. All organisms possess nucleases that promote HJ resolution by the introduction of symmetrically related nicks in two strands at, or close to, the junction point. GEN1, a member of the Rad2/XPG nuclease family, was isolated recently from human cells and shown to promote HJ resolution in vitro and in vivo. Here, we provide the first biochemical/structural characterization of GEN1, showing that, like the Escherichia coli HJ resolvase RuvC, it binds specifically to HJs and resolves them by a dual incision mechanism in which nicks are introduced in the pair of continuous (noncrossing) strands within the lifetime of the GEN1-HJ complex. In contrast to RuvC, but like other Rad2/XPG family members such as FEN1, GEN1 is a monomeric 5'-flap endonuclease. However, the unique feature of GEN1 that distinguishes it from other Rad2/XPG nucleases is its ability to dimerize on HJs. This functional adaptation provides the two symmetrically aligned active sites required for HJ resolution.

  20. Aspectos genéticos y neuroendocrinos en el trastorno del espectro autista

    Directory of Open Access Journals (Sweden)

    Norma Oviedo

    2015-01-01

    Full Text Available El autismo, hoy en día definido como trastornos del espectro autista, fue descrito inicialmente en 1943. Se caracteriza por alteraciones en la comunicación, la interacción social y un espectro restringido de intereses del paciente. Generalmente se identifica en etapas tempranas del desarrollo a partir de los 18 meses de edad. Actualmente el autismo se considera un desorden neurológico con un espectro que abarca diferentes grados que se asocian con factores genéticos, no genéticos y del medio ambiente. Dentro de los factores genéticos se han referido diversos síndromes relacionados con este trastorno. Asimismo, el autismo se ha estudiado a nivel genético, neurofisiológico, neuroquímico y neuropatológico. Las técnicas de neuroimagen han mostrado múltiples anormalidades estructurales en estos pacientes. También se han observado alteraciones relacionadas en los sistemas serotoninérgico, GABAérgico, catecolaminérgico y colinérgico. En este trabajo se presenta una actualización de la información de los aspectos genéticos y neuroendocrinos del trastorno del espectro autista.

  1. Jurados ciudadanos y organismos genéticamente modificados

    Directory of Open Access Journals (Sweden)

    Luque, Emilio

    2005-04-01

    Full Text Available Apolitical sociology of food must look into the processes whereby regulatory decisions on food are made, and also into the democratic potential of their alternatives. Five "experiments in democracy" are described, in which a promising deliberative device has been used: citizens' juries. Indian and Brazilian peasants, on the one hand, and British consumers in the other took part in them, and they focused on Genetically Modified Organisms. These processes, whose defining trait is granting jurors access to expert witnesses presented by all stakeholders in the controversy, show the impressive ability of ordinary citizens to articulate their analysis of complex issues, a reassessment of risks, and a recontextualization of the use of GMOs. At any rate, deliberative democracy is not a magic bullet for the epistemic and political crisis that underlies food crisis; instead, it points at a paradigm change towards an experimental democratic polity in which the instances of representation of the public and publics are multiplied.

    Una sociología política de la alimentación debe analizar los procesos a través de los cuales se alcanzan las decisiones de regulación y control alimentario y examinar el potencial de sus alternativas. Se describen aquí cinco experimentos democráticos en los que se ha empleado uno de los dispositivos deliberativos más prometedores en condiciones de alta complejidad cognitiva, los llamados jurados ciudadanos, con la participación de campesinos hindúes y brasileños y consumidores británicos, y centrados en los Organismos Genéticamente Modificados. Estos procesos, caracterizados por el acceso de los miembros del jurado a testigos expertos presentados por los distintos participantes en la controversia, muestran la enorme capacidad de los ciudadanos "de a pie" para articular el análisis de un problema complejo, reevaluar sus riesgos y recontextualizar el uso de los OGM. En todo caso, la democracia deliberativa no es

  2. Fluviicoccus keumensis gen. nov., sp. nov., isolated from freshwater.

    Science.gov (United States)

    Kim, Min-Kyeong; Kim, Tae-Woon; Kim, Tae-Su; Joung, Yochan; Han, Ji-Hye; Kim, Seung Bum

    2016-01-01

    A Gram-stain-negative, non-spore-forming and coccus-shaped bacterial strain, designated 4DR5T, was isolated from freshwater and its taxonomic position was investigated using a polyphasic approach. Growth occurred at 10-40 °C (optimum 30 °C), at pH 6-9 (optimum pH 7) and in the presence of 0-0.4 % (w/v) NaCl (optimum 0 %) on R2A agar. On the basis of 16S rRNA gene sequence similarity, strain 4DR5T was assigned to the family Moraxellaceae of the class Gammaproteobacteria, and its closest related taxa were species of the genera Perlucidibaca (93.67 % sequence similarity), Agitococcus (93.07 %), Paraperlucidibaca (92.31-92.38 %), Alkanindiges (91.79 %) and Acinetobacter (90.24-91.23 %). The predominant isoprenoid quinone detected in strain 4DR5T was Q-10. The major cellular fatty acids were a summed feature consisting of C16 : 1ω7c and/or C16 : 1ω6c, one consisting of C18 : 1ω7c and/or C18 : 1ω6c, and C16 : 0. The major polar lipid was phosphatidylethanolamine. The genomic DNA G+C content of the strain was 61.2 mol%. The phylogenetic, chemotaxonomic and biochemical data not only supported the affiliation of strain 4DR5T to the family Moraxellaceae, but also separated it from other established genera within the family. Therefore, the novel isolate evidently represents a novel species of a new genus of Moraxellaceae, for which the name Fluviicoccus keumensis gen. nov., sp. nov. is proposed. The type strain of Fluviicoccus keumensis is 4DR5T ( = KCTC 32475T = JCM 19370T).

  3. HIV gp120 vaccine - VaxGen: AIDSVAX, AIDSVAX B/B, AIDSVAX B/E, HIV gp120 vaccine - Genentech, HIV gp120 vaccine AIDSVAX - VaxGen, HIV vaccine AIDSVAX - VaxGen.

    Science.gov (United States)

    2003-01-01

    VaxGen is developing prophylactic vaccines against HIV-1 consisting of two recombinant gp120 surface proteins from different HIV-1 strains.This profile has been selected from R&D Insight, a pharmaceutical intelligence database produced by Adis International Ltd. The bivalent vaccines [AIDSVAX B/B and AIDSVAX B/E] are being evaluated in two phase III trials. The first multicentre phase III trial of AIDSVAX B/B, was conducted principally in Canada and the US but also at some sites in the Netherlands and Puerto Rico. The trial was completed at the end of 2002. The second phase III trial is being conducted in Thailand with the AIDSVAX B/E vaccine. VaxGen announced in July 2002 that it would be delaying its Biologics License Application (BLA) for AIDSVAX until 2004 to enable the company to fulfil pre-approval manufacturing requirements. AIDSVAX is based on an earlier monovalent gp120 vaccine developed by Genentech that was shown to be safe in humans. VaxGen (formerly Genenvax) was formed as a spin-off company from Genentech with the sole purpose of developing the gp120 vaccine. VaxGen announced in July 2002 that the original License and Supply agreement with Genentech, signed in May 1997, had been amended. Under the revised agreement, Genentech maintains its right to market and sell AIDSVAX in North America, but has relinquished its options to commercialise the vaccine candidate in the rest of the world. Genentech's earlier decision to waive its option to manufacture AIDSVAX has also been formalised in this agreement. Additionally, VaxGen's royalty payments to Genentech for sales to the WHO or UN for underdeveloped nations have also been reduced by up to 50% and Genentech has extended the milestone date associated with VaxGen submitting an NDA. A $US120 million joint venture (Celltrion) has been formed between VaxGen and South Korean investors to manufacture more than 200 million doses of AIDSVAX a year. Celltrion will build and operate two biotechnology manufacturing

  4. La incidencia de la genética en el derecho (humano al matrimonio

    Directory of Open Access Journals (Sweden)

    Sergio Romeo Malanda

    2014-10-01

    Full Text Available La idea de evitar el nacimiento de persona con algún tipo de enfermedad ha sido una constante en la historia de la humanidad. Una de las formas de conseguirlo ha sido la de establecer limitaciones a la procreación, tanto directamente, a través de esterilizaciones forzosas, como indirectamente, prohibiendo matrimonios entre personas que no cumplían los requisitos estimados más deseables para tener descendencia sana.Contenido: Introducción. El derecho al matrimonio. Derecho al matrimonio y genética: los diagnósticos genéticos prematrimoniales. Conclusión: los diagnósticos genéticos prematrimoniales respetuosos con los derechos humanos.

  5. La incidencia de la genética en el derecho (humano al matrimonio

    Directory of Open Access Journals (Sweden)

    Sergio Romeo Malanda

    2014-10-01

    Full Text Available La idea de evitar el nacimiento de persona con algún tipo de enfermedad ha sido una constante en la historia de la humanidad. Una de las formas de conseguirlo ha sido la de establecer limitaciones a la procreación, tanto directamente, a través de esterilizaciones forzosas, como indirectamente, prohibiendo matrimonios entre personas que no cumplían los requisitos estimados más deseables para tener descendencia sana.Contenido: Introducción. El derecho al matrimonio. Derecho al matrimonio y genética: los diagnósticos genéticos prematrimoniales. Conclusión: los diagnósticos genéticos prematrimoniales respetuosos con los derechos humanos.

  6. INCORPORACIÓN DEL GEN Mi A VARIEDADES DE TOMATE MEDIANTE EL MARCADOR Aps-1

    OpenAIRE

    2006-01-01

    Frente a los nematodos del género Meloidogyne es la resistencia genética, junto a métodos de lucha integrada, la opción más recomendada para el tomate, por lo que los cultivares e híbridos modernos portan, en su mayoría, el gen Mi, que confiere resistencia no completa a varias especies del género Meloidogyne. El Instituto Nacional de Ciencias Agrícolas (INCA), con la colaboración de la Estación Experimental La Mayora-CSIC, España, ha desarrollado un programa de mejora genética, con el objetiv...

  7. Magnetic behavior in Cr2@Gen (1≤n≤12) clusters: A density functional investigation

    Science.gov (United States)

    Dhaka, Kapil; Trivedi, Ravi; Bandyopadhyay, Debashis

    2014-04-01

    With a goal to produce magnetic moment in Cr2 Doped Gen clusters which will be useful for practical applications, we have considered the structure and magnetic properties of Pure Germanium clusters and substitutionally doped it with Cr dimer to produce Cr2@Gen clusters. As the first step of calculation, geometrical optimizations of the nanoclusters have been done. These optimized geometries have been used in calculate the average binding energy per atom (BE), HOMO-LUMO gap and hence the relative stability of the clusters. These parameters have been demonstrated as structural and electronic properties of the clusters. Gap between highest occupied molecular orbital and lowest unoccupied molecular orbital indicate cluster to be a potential motif for generating magnetic cluster assembled materials. Based on these values a comparative study on different sized clusters has been done in order to understand the origin of structures, electronic and magnetic properties of Cr2@Gen nanoclusters.

  8. Biomek®-3000 and GenPlex in Forensic Genetics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Tomas Mas, Carmen; Hansen, Anders Johannes;

      SNP genotyping provides a supplement for conventional STR-based kits currently used for human identification. GenPlex (Applied Biosystems) is a SNP genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement and valid......  SNP genotyping provides a supplement for conventional STR-based kits currently used for human identification. GenPlex (Applied Biosystems) is a SNP genotyping kit based on a multiplex of 48 informative, autosomal SNPs from the SNPforID Consortium. Our objective was to setup, implement...... with the Biomek-3000 were compared to results obtained with 15 samples that were processed manually. Subsequently, a total of 286 samples were analyzed in duplicates with the GenPlex reaction using the Biomek-3000. The results were compared to those obtained from the same samples using the SNaPshot® single base...

  9. Tools for Designing, Evaluating, and Certifying NextGen Technologies and Procedures: Automation Roles and Responsibilities

    Science.gov (United States)

    Kanki, Barbara G.

    2011-01-01

    Barbara Kanki from NASA Ames Research Center will discuss research that focuses on the collaborations between pilots, air traffic controllers and dispatchers that will change in NextGen systems as automation increases and roles and responsibilities change. The approach taken by this NASA Ames team is to build a collaborative systems assessment template (CSAT) based on detailed task descriptions within each system to establish a baseline of the current operations. The collaborative content and context are delineated through the review of regulatory and advisory materials, policies, procedures and documented practices as augmented by field observations and interviews. The CSAT is developed to aid the assessment of key human factors and performance tradeoffs that result from considering different collaborative arrangements under NextGen system changes. In theory, the CSAT product may be applied to any NextGen application (such as Trajectory Based Operations) with specified ground and aircraft capabilities.

  10. How Does Mentoring Contribute to Gen Y Employees’ Intention to Stay? An Indian Perspective

    Science.gov (United States)

    Naim, Mohammad Faraz; Lenka, Usha

    2017-01-01

    The present study is aimed at investigating the impact of mentoring on intention to stay of Gen Y employees working in Indian IT industry. Also, the mediating roles of perceived organization support and affective commitment are examined. Primary data were collected from a sample of 314 Gen Y employees (born between 1980-2000) from IT industry in Delhi, NCR India. Data analysis was carried out using AMOS and SPSS to test sequential mediation. Findings reveal that mentoring has a direct influence on intention to stay of Gen Y employees and perceived organization support and affective commitment sequentially mediate the relationship between the two. This study contributes to the literature on mentoring, perceived organization support, affective commitment, and intention to stay. PMID:28580029

  11. El problema del discurso acerca de la individualidad genética humana

    OpenAIRE

    Ordóñez Vásquez, Adriana

    2008-01-01

    Se abordó el problema filosófico acerca de la individualidad genética humana, desde un discurso ontológico. Determinándose un problema lógico, epistemológico, semiológico y ontológico, al mismo tiempo que genético. Se presenta el paralelismo entre rasgos de la individualidad señalados por la tradición filosófica clásica y su posible reformulación del discurso desde la genética humana actual. Prueba la pertinencia semiótica de Peirce - biosemiótica en el desarrollo del problema y propone un mo...

  12. rMotifGen: random motif generator for DNA and protein sequences

    Directory of Open Access Journals (Sweden)

    Hardin C Timothy

    2007-08-01

    Full Text Available Abstract Background Detection of short, subtle conserved motif regions within a set of related DNA or amino acid sequences can lead to discoveries about important regulatory domains such as transcription factor and DNA binding sites as well as conserved protein domains. In order to help assess motif detection algorithms on motifs with varying properties and levels of conservation, we have developed a computational tool, rMotifGen, with the sole purpose of generating a number of random DNA or protein sequences containing short sequence motifs. Each motif consensus can be user-defined, randomly generated, or created from a position-specific scoring matrix (PSSM. Insertions and mutations within these motifs are created according to user-defined parameters and substitution matrices. The resulting sequences can be helpful in mutational simulations and in testing the limits of motif detection algorithms. Results Two implementations of rMotifGen have been created, one providing a graphical user interface (GUI for random motif construction, and the other serving as a command line interface. The second implementation has the added advantages of platform independence and being able to be called in a batch mode. rMotifGen was used to construct sample sets of sequences containing DNA motifs and amino acid motifs that were then tested against the Gibbs sampler and MEME packages. Conclusion rMotifGen provides an efficient and convenient method for creating random DNA or amino acid sequences with a variable number of motifs, where the instance of each motif can be incorporated using a position-specific scoring matrix (PSSM or by creating an instance mutated from its corresponding consensus using an evolutionary model based on substitution matrices. rMotifGen is freely available at: http://bioinformatics.louisville.edu/brg/rMotifGen/.

  13. O futuro da epidemiologia genética de características complexas

    Directory of Open Access Journals (Sweden)

    Feitosa Mary F.

    2002-01-01

    Full Text Available A epidemiologia genética evoluiu de um enfoque em estudos sobre doenças mendelianas raras para a análise genética de características complexas. Com o advento de informações sobre a completa seqüência de genes ao longo do genoma humano e de outros organismos, o interesse da epidemiologia genética em desvendar a natureza dos fatores que influenciam essas características se tornou primordial. São apresentados os principais métodos empregados no estudo de doenças complexas bem como suas principais vantagens e desvantagens. Discute-se a importância na determinação da amostra e o uso de fenótipos e marcadores genéticos apropriados. Como exemplo das estratégias citadas tomamos o estudo de índice de massa corporal (BMI para ilustrar um fator genético principal localizado no cromossomo 7. Em uma discussão sobre tendências no estudo de ligação, embora reconhecendo que famílias e genealogias continuarão sendo o foco principal das amostras, discute-se alguns novos e eficientes tipos de amostragem (como por exemplo, controles não-relacionados em que amostras de conjunto de DNA serão universalmente empregadas. O reconhecimento da heterogeneidade genética entre estudos e sua interpretação será uma das mais importantes características no futuro das análises de características complexas.

  14. Loci asociados con enfermedades genéticas y calidad de carne en bovinos Charolais Mexicanos

    Directory of Open Access Journals (Sweden)

    Ana María Sifuentes Rincón

    2015-01-01

    Full Text Available Se determinaron las frecuencias alélicas y genotípicas de ocho marcadores localizados en los genes calpaína (CAPN, 4 751 y 316, calpastatina (CASTT1 y tiroglobulina (TG5, asociados a calidad de carne, y en los genes, m iost atina (MSTN, Q204X, arginino succinato sintasa (ASS, monofosfato sintasa (UMPS y miofosforilasa (PYGM, asocia dos a enfermedade s genéticas de ganado bovino. Se muestrearon 493 animales Charolais de registro de dos hatos ubicado s en Sonora (n=157 y tres en Nuevo León (n=336. No se encontraron portadores de los alelos T-ASS y T-UM PS, pero sí portadores del alelo Q204X del gen MSTN en frecuencias de  1 % en las poblaciones de Sonora y de 8.6 a 14.4 % en las de Nuevo León. Además, se identificaron portadores del marcador del gen PYGM, en frecuencias del 6.5 y de 1.0 % para un hato de Sonora y otro de Nuevo León, respectivamente. El análisis de diferenciación génica p areado entre las poblaciones y con los cuatro loci mostró que hay diferencias altamente significativas dentro de pobl aciones del noroeste ( P <0.0001 y entre éstas y las del noreste ( P <0.001, la cual es explicada principalmente por los loci CAPN-316 y TG5. De acuerdo a los resultados obtenidos se recomienda el monitoreo del marcador del gen PYGM y del ale lo Q204X del gen MSTN, así como también implementar estrategias para confirmar la utilidad de los marcadores asociado s a calidad y productividad como herramienta para complementar los progra mas de mejoramiento genético.

  15. VARIABILIDAD DEL GEN NUCLEAR G3PDH EN JATROPHA CURCAS L.

    Directory of Open Access Journals (Sweden)

    Sonia Castro Guzmán

    2015-11-01

    Full Text Available Jatrophacurcas L.es una especie nativa de América tropical; en nuestro país se ha venido utilizando principalmente como medicinal y alimenticia desde la época prehispánica. Actualmente el aceite extraído de sus semillas ha adquirido importancia internacional ya que puede ser transformado a biodiesel. El conocimiento que existe sobre la variabilidad genética de la especie es escaso. Un gen utilizado con éxito para el estudio de patrones de variación y origen de la yuca (ManihotesculentaL. y del caco (Theobromacacao L. es el gen nuclearG3pdh(Gliceraldehído3 fosfato deshidrogenasa involucrado en la fotosíntesis. Con base en ello, en este trabajo se exploró la variabilidad del gen G3pdh en individuos deJ. curcasprovenientes de 15 poblaciones de los estados de Veracruz, Campeche, Yucatán y Quintana Roo. Para obtener el gen G3pdh completo de alrededor de 1000 pares de bases, se amplificó utilizando losprimersdiseñados porStrandet al. (1997 y los dosprimersinternos reverse diseñados porOlsenySchaal(1999 para obtener segmentos más cortos, de 600 y 800 pares de bases aproximadamente. Por primera vez se amplificaron aproximadamente 500pby los resultados demuestran que el gen G3pdh es útil para analizar la variabilidad deJ. curcas, y con un importante potencial para evaluar la distribución y evolución de sus poblaciones en México, conocer las relaciones ancestro descendiente a nivel poblacional y explicar las causas de la distribución de los distintoshaplotipos.

  16. SIPSMetGen: It's Not Just For Aircraft Data and ECS Anymore.

    Science.gov (United States)

    Schwab, M.

    2015-12-01

    The SIPSMetGen utility, developed for the NASA EOSDIS project, under the EED contract, simplified the creation of file level metadata for the ECS System. The utility has been enhanced for ease of use, efficiency, speed and increased flexibility. The SIPSMetGen utility was originally created as a means of generating file level spatial metadata for Operation IceBridge. The first version created only ODL metadata, specific for ingest into ECS. The core strength of the utility was, and continues to be, its ability to take complex shapes and patterns of data collection point clouds from aircraft flights and simplify them to a relatively simple concave hull geo-polygon. It has been found to be a useful and easy to use tool for creating file level metadata for many other missions, both aircraft and satellite. While the original version was useful it had its limitations. In 2014 Raytheon was tasked to make enhancements to SIPSMetGen, this resulted a new version of SIPSMetGen which can create ISO Compliant XML metadata; provides optimization and streamlining of the algorithm for creating the spatial metadata; a quicker runtime with more consistent results; a utility that can be configured to run multi-threaded on systems with multiple processors. The utility comes with a java based graphical user interface to aid in configuration and running of the utility. The enhanced SIPSMetGen allows more diverse data sets to be archived with file level metadata. The advantage of archiving data with file level metadata is that it makes it easier for data users, and scientists to find relevant data. File level metadata unlocks the power of existing archives and metadata repositories such as ECS and CMR and search and discovery utilities like Reverb and Earth Data Search. Current missions now using SIPSMetGen include: Aquarius, Measures, ARISE, and Nimbus.

  17. Genealogia e heredogramas como ferramentas no ensino da genética mendeliana

    OpenAIRE

    Manzke, Vítor; Bobrowski,Vera Lucia; Manzke, Gabriela; Rodrigues,Maria José

    2012-01-01

    Este trabalho foi desenvolvido a partir do uso da genealogia como ferramenta auxiliar ao estudo da genética mendeliana nos contextos do mono e do diíbridismo. Para isso os alunos passaram a estabelecer relações entre o grau de parentesco dos membros de seu grupo familiar e a identificar a manifestação de características genéticas pré-estabelecidas em comum acordo entre o professor e os alunos. Nosso objetivo foi oportunizar aos alunos a sistematização do grupo familiar, em cinco gerações, ten...

  18. Análisis del gen SOX-2 en carcinoma escamoso de cabeza y cuello

    OpenAIRE

    González Márquez, Rocío

    2015-01-01

    El carcinoma escamoso de cabeza y cuello es la sexta neoplasia más frecuente en el mundo. El origen de dichos tumores implica gran numero de alteraciones genéticas y moleculares, siendo recientemente estudiado el gen SOX2. Se estudiaron las muestras procedentes de 220 pacientes con carcinoma escamoso de hipofaringe, laringe y senos paranasales. En el estudio inmunohistoquímico apareció un grado de sobreexpresión similar en tumores faringeos y laríngeos, así como relación significativa con...

  19. Análisis del gen SOX-2 en carcinoma escamoso de cabeza y cuello

    OpenAIRE

    González Márquez, Rocío

    2014-01-01

    El carcinoma escamoso de cabeza y cuello es la sexta neoplasia más frecuente en el mundo. El origen de dichos tumores implica gran numero de alteraciones genéticas y moleculares, siendo recientemente estudiado el gen SOX2. Se estudiaron las muestras procedentes de 220 pacientes con carcinoma escamoso de hipofaringe, laringe y senos paranasales. En el estudio inmunohistoquímico apareció un grado de sobreexpresión similar en tumores faringeos y laríngeos, así como relación significativa con...

  20. Introgresión genética (IG en ganado criollo colombiano (GCC

    Directory of Open Access Journals (Sweden)

    Zuluaga FN.

    2001-06-01

    Full Text Available Determinar variabilidad y estimar los niveles de mestizaje en las razas de GCC mediante laevaluación de marcadores polimórficos en ADN mitocondrial y cromosoma Y y analizar filo-genéticamente el origen del GCC.Las siete razas de GCC han sufrido procesos de adaptación a las condiciones tropicales y hanacumulado características genéticas únicas y de gran valor, principalmente de tipo reproductivoe inmunológico.

  1. Avances genéticos y moleculares en el estudio de trastornos mentales

    OpenAIRE

    Daisy Natalia Salamanca-Ortíz; Jorge Yamit Vergara-Vergara; Franklin Escobar-Córdoba; Álvaro Rodríguez-Gama; Jorge Eduardo Caminos- Pinzón

    2014-01-01

    Actualmente, con el aumento de la disponibilidad de técnicas para la realización de estudios genéticos, han aparecido nuevas áreas del conocimiento como la epigenética y la farmacogenética. Estas nuevas áreas han permitido esclarecer las bases genéticas implicadas en la aparición de las enfermedades que afectan al ser humano. Dada su aplicación global, la psiquiatría no ha sido ajena al alcance de estas nuevas disciplinas, lo cual se refleja en el gran número de estudios realizados a nivel mu...

  2. LabSystem Gen, a tool for structuring and analyzing genetic data in histocompatibility laboratories.

    Science.gov (United States)

    Sousa, Luiz Cláudio Demes da Mata; dos Santos Neto, Pedro de Alcântara; de Souza, Fernando da Fonseca; do Monte, Semiramis Jamil Hadad

    2012-04-01

    Analysis of HLA data involves queries on web portals, whose search parameters are data stored in laboratories' databases. In order to automate these queries, one approach is to structure laboratory data into a database and to develop bioinformatic tools to perform the data mapping. In this context, we developed the LabSystem Gen tool that allows users to create a Laboratory Information System, without programming. Additionally we implemented a framework that provides bioinformatic tools, transparent access to public HLA (human leukocyte antigen) information resources. We demonstrated the LabSystemGen system by implementing BMDdb, which is a LIMS that manages data of recipients and donors of organ transplant.

  3. 不是木马的“木马”——VBS:Obfuscated-gen [Trj

    Institute of Scientific and Technical Information of China (English)

    陈小虎

    2009-01-01

    今天,单位上网网速奇慢,一会儿就断线,打开网页avast弹出提示发现病毒“VBS:Obfuscated—gen[Trj]”,打开任何一个网页,都出现“VBS:Obfuscated—gen[Trj]”的病毒提示,很多网页根本就打不开。其他同事也反映上网出现病毒提示,网速慢得吓人……

  4. LEPINOCONUS CHIOCCHINII GEN. N., N. SP., A CONICAL AGGLUTINATED FORAMINIFERA FROM THE UPPER CRETACEOUS OF ITALY

    Directory of Open Access Journals (Sweden)

    ERZIKA CRUZ-ABAD

    2017-04-01

    Full Text Available A new conical agglutinated foraminifer, Lepinoconus chiocchinii gen n., n. sp. from the lower Campanian shallow-water platform deposits of the Lepini Mountains (central Apennines, Italy, is described. It has a pseudo-keriothecal wall structure, uniserial arrangement of the adult chambers and multiple apertures. The exoskeleton is constituted by beams (main and intercalary continuous from one chamber to the next, while the endoskeleton bears pillars. The new taxon is included in the Coskinolinidae family. Lepinoconus chiocchinii gen. n., n. sp. is known from southern Italy, Greece and Albania.

  5. MLPA en el estudio de desórdenes genómicos

    OpenAIRE

    Ramírez, Jésica; Echeverría, María; Mampel, Alejandra; Marino, Miguel; Gallardo, A.; Triguy, J.; Schroh, A.; Arce, Cecilia; Marzese, Diego; Calderón, Enrique; Vargas, Ana

    2009-01-01

    El término de desórdenes genómicos se utiliza para definir aquellas condiciones que surgen por inestabilidad en la molécula de ADN y, que ocasionan, rearreglos cromosómicos que involucran regiones de uno o varios pares de megabases. Estos rearreglos determinan la pérdida, ganancia o disrupción de genes cuya expresión fenotípica varía de acuerdo a la cantidad de secuencia codificante presente (dosage- sensitive- genes). Estas anormalidades genó...

  6. Biochemical and molecular characterization of the gentisate transporter GenK in Corynebacterium glutamicum.

    Directory of Open Access Journals (Sweden)

    Ying Xu

    Full Text Available BACKGROUND: Gentisate (2,5-dihydroxybenzoate is a key ring-cleavage substrate involved in various aromatic compounds degradation. Corynebacterium glutamicum ATCC13032 is capable of growing on gentisate and genK was proposed to encode a transporter involved in this utilization by its disruption in the restriction-deficient mutant RES167. Its biochemical characterization by uptake assay using [(14C]-labeled gentisate has not been previously reported. METHODOLOGY/PRINCIPAL FINDINGS: In this study, biochemical characterization of GenK by uptake assays with [(14C]-labeled substrates demonstrated that it specifically transported gentisate into the cells with V(max and K(m of 3.06 ± 0.16 nmol/min/mg of dry weight and 10.71 ± 0.11 µM respectively, and no activity was detected for either benzoate or 3-hydoxybenzoate. When GenK was absent in strain RES167 ΔgenK, it retained 85% of its original transport activity at pH 6.5 compared to that of strain RES167. However, it lost 79% and 88% activity at pH 7.5 and 8.0, respectively. A number of competing substrates, including 3-hydroxybenzoate, benzoate, protocatechuate and catechol, significantly inhibited gentisate uptake by more than 40%. Through site-directed mutagenesis, eight amino acid residues of GenK, Asp-54, Asp-57 and Arg-386 in the hydrophobic transmembrane regions and Arg-103, Trp-309, Asp-312, Arg-313 and Ile-317 in the hydrophilic cytoplasmic loops were shown to be important for gentisate transport. When conserved residues Asp-54 and Asp-57 respectively were changed to glutamate, both mutants retained approximately 50% activity and were able to partially complement the ability of strain RES167 ΔgenK to grow on gentisate. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that GenK is an active gentisate transporter in Corynebacterium glutamicum ATCC13032. The GenK-mediated gentisate transport was also shown to be a limiting step for the gentisate utilization by this strain. This enhances our

  7. Riesgos sobre la salud de los alimentos modificados genéticamente: una revision bibliografica

    OpenAIRE

    2000-01-01

    A lo largo de 1999, se ha venido intensificando el debate sobre la seguridad de los alimentos modificados genéticamente, una importante y compleja área de investigación científica, la cual demanda unos estándares rigurosos. Diversos grupos, incluyendo asociaciones de consumidores y Organizaciones no Gubernamentales (ONGs) han sugerido que todos los alimentos modificados genéticamente deberían ser sometidos a estudios a largo plazo con animales antes de su aprobación para el consumo humano. El...

  8. A psicologia face aos novos progressos da genética humana

    OpenAIRE

    Rossano André Dal-Farra; Emerson Juliano Prates

    2004-01-01

    Os avanços da Genética contemporânea têm suscitado uma grande polêmica a respeito da gênese do homem e das possibilidades de agir sobre a biologia humana, repercutindo intensamente sobre os mais diversos ramos do conhecimento. Considerando a importância do tema, este texto pretende apresentar de forma sucinta os principais resultados de pesquisas sobre as bases genéticas do comportamento humano, com o foco voltado para a importância do profissional de Psicologia em questões relativas ao assun...

  9. Alimentos Transgénicos : Organismos Genéticamente Modificados (OGM)

    OpenAIRE

    Martín López, Jimena

    2016-01-01

    Los alimentos transgénicos son aquellos que proceden de un organismo modificado genéticamente. La introducción de este tipo de productos en nuestra dieta es un tema que genera controversia ya que en muchos casos no se conoce con exactitud los efectos que esta modificación puede tener en el ser humano. A lo largo de las páginas de este trabajo se explica la historia de la aparición de estos organismos gracias a procedimientos de ingeniería genética, en los que se modifican fragmentos de su ADN...

  10. Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    2011-06-01

    Full Text Available Melostelis gen. nov., espécies novas e notas complementares sobre Anthidiini (Hymenoptera, Apidae. Melostelis gen. nov. é proposto para um novo Anthidiini cleptoparasita. São descritas e ilustradas duas espécies novas: Melostelis amazonensis sp. nov. de Manaus, Amazonas e Larocanthidium chacoense sp. nov. de Porto Murtinho, Mato Grosso do Sul. São dados a conhecer os machos de Epanthidium bolivianum Urban, 1995 e Epanthidium araranguense Urban, 2006 e, registrados pela primeira vez no Brasil, na sub-região do chaco, Ketianthidium zanolae Urban, 2000 e Epanthidium bolivianum.

  11. Estrategias de genética reversa aplicadas al vesivirus de conejo

    OpenAIRE

    García Manso, Alberto

    2016-01-01

    En el ámbito de la Virología, se denomina genética reversa al proceso de rescate de virus RNA de novo a partir de una copia del genoma viral en forma de cDNA. El trabajo recogido en esta memoria de tesis doctoral se ha dirigido a la obtención de un sistema de genética reversa para el vesivirus de conejo (RaV), uno de los pocos calicivirus capaz de ser propagado en cultivos celulares. Para ello, se han utilizado diferentes estrategias para expresar distintos clones de cDNA sintetizados. En ...

  12. Régimen de propiedad sobre recursos genéticos y conocimiento tradicional

    OpenAIRE

    2011-01-01

    Este artículo expone los cambios históricos que se han dado en relación con la propiedad, el control y el acceso a los recursos genéticos. Desde su consideración como patrimonio común de la humanidad, los derechos sobre el material y la información genética han sufrido profundas transformaciones. El marco jurídico internacional concerniente a estos derechos se ha transformado para legitimar y promover su apropiación y monopolización. Parte de esta transformación fue la expansión de derechos d...

  13. Ingeniería genómica mediante sistemas CRISPR-Cas

    OpenAIRE

    López Mancheño, Yaiza Araceli

    2015-01-01

    Desde su descubrimiento, a finales de la década de los ochenta, hasta la actualidad, las secuencias denominadas CRISPR, se han revelado como instrumentos a través de los cuáles provocar cambios dirigidos en secuencias génicas de interés. El conocimiento de su función y actuación natural en microorganismos permitió a los científicos, sospechar sus posibles aplicaciones como herramienta de edición genómica ya que el sistema era capaz de reconocer elementos genéticos externos, incorporarlos y, t...

  14. Análisis genético del virus peruano de la fiebre amarilla

    OpenAIRE

    Carlos Yábar V; Yván Campos B; Kelly Quispe T; Carlos Carrillo P; Ysabel Montoya P

    2002-01-01

    Objetivo: Determinar las variantes genéticas de aislamientos del virus peruano de la Fiebre Amarilla (FA). Materiales y métodos: la región carboxiterminal del gen de la envoltura (E) de cinco aislamientos de FA obtenidas de pacientes provenientes de Ayacucho 1978 (PER1), Junín 1995 (PER2), Cerro de Pasco (PER3), Cusco (1998) y San Martín (1999) fue amplificada por PCR, secuenciada y analizada con programas software de ADN. Resultados: el índice de similaridad de la secuencia de nucleótidos en...

  15. Ilegalidad de la investigación genética en colombia

    OpenAIRE

    Gómez, Diana; Nemogá Soto, Gabriel

    2013-01-01

    El marco legislativo colombiano que regula el acceso a los recursos genéticos y el uso de la diversidad biológica con fines investigativos se ha convertido en un obstáculo para el desarrollo de los procesos investigativos en Colombia. Con el ánimo de conocer cuáles son realmente los problemas con que se enfrentan los investigadores se realizó una revisión de las solicitudes de acceso a recursos genéticos y permisos de investigación que se tramitan ante el Ministerio de Ambiente, Vivienda y...

  16. Microsatélites, distancias genéticas y estructura de poblaciones nativas sudamericanas

    Directory of Open Access Journals (Sweden)

    Demarchi, Darío Alfredo

    2009-01-01

    Full Text Available En este trabajo se investigaron las relaciones genéticas entre 17 poblaciones nativas sudamericanas en relación a 15 microsatélites (STRs autosómicos, utilizando 3 distancias genéticas- DST, DAy (δu2-que se ajustan a diferentes postulados teóricos. A través de diferentes técnicas de análisis (escalamiento multidimensional, correlación y correlación parcial de matrices se puso a prueba si las distancias genéticas reflejaban las relaciones interpoblacionales esperadas a partir de la distribución geográfica o de relaciones lingüísticas entre las poblaciones. Además, se estimó en que grado las distintas medidas de distancias genéticas eran influenciadas por la diversidad (He de cada población. Los mapas genéticos muestran, principalmente para DST y DA, que las poblaciones aisladas y con bajo tamaño efectivo (Ne aparecen como outliers, mientras que las poblaciones con alto Ne y mayor flujo génico ocupan una posición central a bajos valores de distancia unas de otras y sin un patrón definido de agrupamiento. La falta de asociación entre distancias genéticas y lingüísticas o geográficas y por otra parte, la alta correlación negativa entre He y distancias génicas promedio por población confiman ese patrón, demostrando que la mayor parte de la variación interpoblacional puede ser explicada en función del grado de diversidad intrapoblacional. Es decir, las distancias genéticas no reflejan relaciones filogenéticas, lingüísticas o geográficas, sino más bien eventos demográficos recientes tales como cuellos de botella genético, efecto fundador o migración externa masiva. Este hecho puede ser comprobado por medio de otra metodología analítica, el modelo de Harpending y Ward.

  17. A psicologia face aos novos progressos da genética humana

    OpenAIRE

    Rossano André Dal-Farra; Emerson Juliano Prates

    2004-01-01

    Os avanços da Genética contemporânea têm suscitado uma grande polêmica a respeito da gênese do homem e das possibilidades de agir sobre a biologia humana, repercutindo intensamente sobre os mais diversos ramos do conhecimento. Considerando a importância do tema, este texto pretende apresentar de forma sucinta os principais resultados de pesquisas sobre as bases genéticas do comportamento humano, com o foco voltado para a importância do profissional de Psicologia em questões relativas ao assun...

  18. Variabilidad genética y gestión forestal

    OpenAIRE

    Alía, R.; D. Agúndez; Alba, N; S.C. González Martínez; A. Soto

    2003-01-01

    La diversidad biológica, su conservación e incremento, es uno de los principios básicos de la gestión forestal sostenible. La alta variabilidad genética de las especies forestales es responsable de los procesos de adaptación ante factores bióticos y abióticos extremos que, a su vez, aseguran la persistencia frente a los riesgos a los que están sometidas las masas forestales. En este trabajo se analizan distintos aspectos relacionados con la variabilidad genética de las especies fores...

  19. Detecció i estudi de gens implicats en el desenvolupament prenatal de la rata

    OpenAIRE

    2002-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Introducció El treball "Detecció i estudi de gens implicats en el desenvolupament prenatal de la rata" parteix de la hipòtesi de treball de què la carcinogènesi i el desenvolupament són processos que comparteixen mecanismes de funcionament com són l'elevat índex de proliferació, la capacitat de migració cel·lular o l'angiogènesi sostinguda i que, per tant, deuen compartir els gens que els regulen. Amb aquest plantejament...

  20. Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae), new nematode genera as parasites of Passalidae (Coleoptera) from the Democratic Republic of Congo.

    Science.gov (United States)

    Morffe, Jans; García, Nayla

    2013-01-01

    Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973) comb. n. and B. annulatum (Van Waerebeke, 1973) comb. n. Chokwenema lepidophorum gen. n. et sp. n. is characterized by having females with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema) that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic.

  1. Batwanema gen. n. and Chokwenema gen. n. (Oxyurida, Hystrignathidae, new nematode genera as parasites of Passalidae (Coleoptera from the Democratic Republic of Congo

    Directory of Open Access Journals (Sweden)

    Jans Morffe

    2013-12-01

    Full Text Available Two new genera and species parasitizing passalid beetles from the Democratic Republic of Congo are described. Batwanema congo gen. n. et sp. n. is characterized byfemales with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements that gradually divide and form pointed spines toward the end of the spiny region, two cephalic annuli, clavate procorpus and genital tract monodelphic-prodelphic. Two Malagasian species of Artigasia Christie, 1934 were placed in this genus as B. latum (Van Waerebeke, 1973 comb. n. and B. annulatum (Van Waerebeke, 1973 comb.n. Chokwenema lepidophorum gen. n. et sp. n. is characterized byfemales with the cervical cuticle armed with scale-like projections, arranged initially in rows of eight elements (similar to Batwanema that divide gradually, forming spines; a single cephalic annule cone-like, truncated, moderately inflated; procorpus sub-cylindrical and genital tract didelphic-amphidelphic.

  2. Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

    Science.gov (United States)

    Breider, Sven; Scheuner, Carmen; Schumann, Peter; Fiebig, Anne; Petersen, Jörn; Pradella, Silke; Klenk, Hans-Peter; Brinkhoff, Thorsten; Göker, Markus

    2014-01-01

    Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria) genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus, and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov., and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.

  3. Resistência genética à mancha-bacteriana em genótipos de pimentão

    OpenAIRE

    Costa Roberto Alexandre; Rodrigues Rosana; Sudré Cláudia Pombo

    2002-01-01

    A mancha-bacteriana, principal doença bacteriana do pimentão causa desfolha intensa quando em condições favoráveis, deixando os frutos expostos ao sol, depreciando-os e diminuindo a produção. Para estimar, nas condições de Campos dos Goytacazes, os efeitos genéticos da reação do hospedeiro ao patógeno, tanto em folhas como em frutos, foram obtidos híbridos F1, sem recíprocos, a partir de cruzamentos dialélicos entre cinco genótipos de pimentão, sendo três suscetíveis ('UENF 1420', 'UENF 1421'...

  4. Genetic diversity in Australian Cedar genotypes selected by mixed models Diversidade genética em genótipos de Cedro Australiano selecionados via modelos mistos

    Directory of Open Access Journals (Sweden)

    Rulfe Tavares

    2012-04-01

    Full Text Available The increasing demand for raw material for multiple uses of forest products and by-products has attracted the interest for fast growing species, such as the Australian Cedar (Toona ciliata, which presents high productive and economic potential. The present work aimed at estimating genetic diversity by DNA markers and morphological traits supported for the mixed models. The following traits were measured and genotypes were sampled randomly in different areas: diameter at breast height, height, cylindrical volume, diameter, distance between nodes and crown diameter. Twelve RAPD primers were used and generated a total of 91 marks, 82 of which were polymorphic. The high percentage of polymorphic markers, 90.10%, demonstrated that discrimination in this species is efficient, but it yet little studied, for this case we can find the extent of the genetic basis for the application of technical improvement. The assessment of genetic diversity by the UPGMA method using the binary and morphological data provided the expression of genetic dissimilarities among the accessions evaluated, optimizing the perception of this divergence. The use of mixed models was efficient to assess combined genetic diversity to optimize the selection of genotypes with divergent genetic values for diameter at breast height.A crescente demanda por matéria-prima para múltiplos usos dos produtos florestais e subprodutos tem despertado o interesse para espécies de crescimento rápido, como o cedro australiano (Toona ciliata, que apresenta potencial produtivo e econômico. Neste trabalho, objetivou-se estimar a diversidade genética por marcadores de DNA e caracteres morfológicos com o uso dos modelos mistos. Os seguintes caracteres foram medidos e os genótipos foram amostrados aleatoriamente em diferentes áreas de plantio: diâmetro à altura do peito, altura, volume cilíndrico, distância entre nós e diâmetro de copa. Doze primers RAPD foram utilizados e geraram um total

  5. História da genética no Brasil: um olhar a partir do Museu da Genética da Universidade Federal do Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Vanderlei Sebastiao de Souza

    2013-06-01

    Full Text Available Aborda o contexto de criação do Museu da Genética, em 2011 no Departamento de Genética na Universidade Federal do Rio Grande do Sul, em Porto Alegre, e apresenta sua estrutura e conteúdo. Argumenta-se que os materiais disponibilizados no Museu da Genética constituem uma rica fonte para pesquisas sobre a história da genética no Brasil (e da genética de populações humanas em particular a partir da segunda metade do século XX, tema ainda pouco investigado, apesar da proeminência dessa área do conhecimento no Brasil.

  6. Frecuencia de discapacidad en estudiantes que presentan la prueba estandarizada de acceso a la educación superior (Saber 11 en Colombia y caracterización de su rendimiento The frequency of disability amongst highschool students taking the standardised Colombian highschool leaving test (Saber 11 and a characterisation of their performance

    Directory of Open Access Journals (Sweden)

    Andrea PadillaMuñoz

    2012-12-01

    Full Text Available Introducción. El examen nacional de acceso a la educación superior en Colombia (prueba Saber 11, es obligatorio para obtener el título de bachiller y ha realizado adaptaciones que permiten evaluar a personas con discapacidad física, auditiva y visual. Objetivo. Describir las características y el rendimiento en la prueba Saber 11 de las personas con discapacidad (PCD en 2009. Materiales y métodos. Análisis secundario de tipo descriptivo de los resultados del examen nacional de acceso a la educación superior (prueba Saber 11, presentado por PCD en el ano 2009. La certificación de discapacidades realizada previa al examen durante la inscripción a la prueba. Se describen las características de dicha población, junto con los puntajes en la prueba. Resultados. 529.651 estudiantes presentaron la prueba por primera vez, de los cuales el 0,2% (842 afirmaron durante la inscripción tener algún tipo de discapacidad. Del total de PCD, el 35,0% presentó una discapacidad motora, el 34,9% presentó una discapacidad auditiva y requirieron intérprete en la prueba, 19,1% presentó una discapacidad visual y un 11,5% presentó discapacidad auditiva, pero no requirieron intérprete. La mayoría de sus puntajes están en categorización media, y, en promedio, las personas con discapacidad visual tuvieron resultados mejores en las áreas básicas al compararlos con otras discapacidades. Conclusión. Este estudio sugiere que son pocas las PCD que se presentan a la prueba Saber 11. No existe forma de evidenciar discapacidad cognitiva en la prueba.Background. Taking the standardised Colombian highschool leaving test (Saber 11 is obligatory for students to graduate from high school and is also used for rating students applying to Colombian universities; it has been adapted for evaluating young people having hearing physical, visual and auditory disabilities. Objectives. Describing and characterising the profile of students suffering from disability (SWD who

  7. 75 FR 1052 - Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of...

    Science.gov (United States)

    2010-01-08

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Terra-Gen Dixie Valley, LLC; TGP Dixie Development Company, LLC; New York Canyon, LLC; Notice of Filing December 30, 2009. Take notice that on December 24, 2009, Terra-Gen...

  8. Discourses in Conflict: The Relationship between Gen Y Pre-Service Teachers, Digital Technologies and Lifelong Learning

    Science.gov (United States)

    Donnison, Sharn

    2009-01-01

    This paper analyses Gen Y pre-service primary school teachers' conceptualisations of lifelong learning. It is situated within a context of improving the provision and delivery of pre-service teacher education. This paper argues that Gen Y's understanding of lifelong learning has been influenced by their engagements with digital technologies and…

  9. Autosomal SNP typing of forensic samples with the GenPlex(TM) HID System: Results of a collaborative study

    DEFF Research Database (Denmark)

    Tomas, C.; Axler-DiPerte, G.; Budimlija, Z.M.

    2011-01-01

    The GenPlex(TM) HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex(TM) HID System using 250-500 pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in ...

  10. Microcenoscelis n. gen. (Coleoptera: Tenebrionidae: Ulomini) from tropical Africa, with description of a blind species from Zimbabwe.

    Science.gov (United States)

    Schawaller, Wolfgang

    2015-10-05

    Microcenoscelis n. gen. (Coleoptera: Tenebrionidae: Ulomini) caeca n. sp. is described from Zimbabwe, a small and completely blind species. A second known species, however with completely developed eyes, and originally described as Uloma minuscula Ardoin, 1969, was also placed in the new genus. Microcenoscelis n. gen. seems to be mostly related to the genera Cenoscelis Wollaston, 1867, and Cneocnemis Gebien, 1914.

  11. Zebra Delivers EPC Gen 2 RFID Printing/Encoding With Industry’s First Free,Downloadable Firmware Upgrade

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Companies can add Gen2 support on existing Zebra RFID printer/encoders while continuing to use Gen 1 and other protocols: no new hardware required. Vernon Hills,Ⅲ., December 14,2005-Zebra Technologies (Nasdaq:ZBRA),a leading manufacturer of radio frequency identification (RFID) printer/encoders worldwide, today

  12. Astioglossimetra karachiensis n. gen., n. sp. (Trematoda: Plagiorchiidae: Astiotrematinae) from the Marine Turtle Chelonia mydas of Karachi Coast

    OpenAIRE

    BILQEES, Fatima Mujib

    2002-01-01

    A new trematode genus Astioglossimetra n. gen. has been erected to accommodate an undescribed species A. karachiensis n. gen., n. sp., from Chelonia mydas of the Karachi coast. The genus name refers to its relation to the genera Astiotrema Looss and Glossimetra Mehra of the family Plagiorchiidae and subfamily Astiotrematinae.

  13. Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov.

    Science.gov (United States)

    Nedashkovskaya, Olga I; Kim, Seung Bum; Lysenko, Anatoly M; Frolova, Galina M; Mikhailov, Valery V; Lee, Kang Hyun; Bae, Kyung Sook

    2005-01-01

    The taxonomic position of three novel sea-water isolates was determined. The strains studied were strictly aerobic, heterotrophic, pigmented, motile by gliding, Gram-negative and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. 16S rRNA gene sequence phylogenetic analysis indicated that the strains KMM 6020T, KMM 6021 and KMM 6028 occupied a distinct lineage within the family Flavobacteriaceae. The major respiratory quinone was MK-6. The predominant fatty acids were i15 : 0, i15 : 1, i15 : 0 3-OH, i17 : 1omega9c and i17 : 0 3-OH. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacteria were assigned to the genus Aquimarina gen. nov., as Aquimarina muelleri gen. nov., sp. nov. The type strain is KMM 6020T (=KCTC 12285T=LMG 22569T). From the results of the 16S rRNA gene sequence analysis and phenotypic features, the species [Cytophaga] latercula Lewin 1969 is proposed to be reclassified in the new genus Stanierella as Stanierella latercula gen. nov., comb. nov., with type strain CIP 104806T (=ATCC 23177T=NCIMB 1399T=LMG 1343T).

  14. Discusión: Explicaciones genéticas y psicológicas de la esquizofrenia. Esquizofrenia, genética y complejidad

    Directory of Open Access Journals (Sweden)

    Henning Jensen Pennington

    2003-01-01

    Full Text Available El artículo plantea la necesidad de no sobresimplificar fenómenos complejos como el de la esquizofrenia, pues su etiología implica la interacción de aspectos genéticos, biológicos y sociopsicológicos. Se plantea que el mismo conocimiento genético imposibilita la asunción de estas posiciones radicales, razón por la cual la investigación biológica actúa con cautela en la formulación de hipótesis causales. No obstante, se pone de manifiesto la interpretación inapropiada de los resultados de investigaciones genéticas y biológicas, cuando por un lado, se postula la complejidad del fenómeno, pero por otro lado y por un acto de simplificación cognoscitiva, se desatiende esa complejidad y se da el salto hacia explicaciones monocausales. La actividad investigativa en el campo de la esquizofrenia se enfrenta entonces a la responsabilidad histórica de dar a cada elemento el lugar que le corresponde en la intrincada red de elementos que influyen en la aparición y desarrollo de la esquizofrenia.

  15. Chitinozoan faunas from the Rügen Ordovician (Rügen 5/66 and Binz 1/73 wells), NE Germany.

    Science.gov (United States)

    Samuelsson, J; Verniers, J; Vecoli, M

    2000-12-01

    The island of Rügen (NE Germany), situated close to the Trans-European Suture Zone (TESZ), in the southern Baltic Sea is underlain by sedimentary rocks of an Early Palaeozoic age, known only from boreholes. The wells, Rügen 5/66 and Binz 1/73, were investigated for their chitinozoan assemblages to improve the earlier biostratigraphic dating (graptolites and acritarchs) and to facilitate comparisons with other chitinozoan assemblages on both sides of the TESZ. In the lower part of the Rügen 5/66 core (3794.7-3615.8m), Lagenochitina destombesi Elaouad-Debbaj is indicative of an early late Tremadoc age. In the upper part of the same well (3287.3-1709.7m), the observed chitinozoan taxa suggest an age spanning the early Llanvirn to the Caradoc. The entire sampled interval of the Binz 1/73 core (5217.6-5041.8m) is interpreted as belonging to the Siphonochitina formosa Biozone (early-early late Abereiddian, corresponding to the early Llanvirn). The chitinozoan data corroborate the earlier suggested biostratigraphic ages, based on acritarchs and graptolites. The chitinozoans from the Binz 1/73 well point to a high latitude provenance of the investigated host sediments at time of deposition.

  16. O impacto da genética na asma infantil Impact of genetics in childhood asthma

    Directory of Open Access Journals (Sweden)

    Leonardo A. Pinto

    2008-08-01

    Full Text Available OBJETIVO: Apresentar os resultados dos estudos mais importantes e recentes sobre a genética da asma. Estes dados devem auxiliar os clínicos gerais a compreender o impacto da genética sobre este distúrbio complexo e como os genes e polimorfismos influenciam a asma e a atopia. FONTES DOS DADOS: Os dados foram coletados do banco de dados MEDLINE. Os estudos de associação genética foram selecionados do Genetic Association Database, um repositório de estudos de associação genética de doenças e distúrbios complexos organizado pelo National Institutes of Health. SÍNTESE DOS DADOS: Considerando os dados de diversos importantes estudos de gêmeos sobre a genética da asma, a heritabilidade, que mensura a contribuição dos fatores genéticos para a variância da asma, pode ser estimada entre 0,48 e 0,79. Uma grande quantidade de estudos de associação genética tentou identificar genes de susceptibilidade à asma. Os resultados mais replicados nos estudos de associação genética envolvem as cinco regiões do genoma humano a seguir: 5q31-32, 6p21, 11q12-13, 16p11-12, e 20p13. Recentemente, outro gene de susceptibilidade à asma (ORMDL3, considerado determinante crítico para a asma infantil, foi identificado por um estudo genômico de associação. CONCLUSÕES: É possível estimar que a contribuição genética à asma varia entre 48 e 79%. Diversos loci parecem influenciar a susceptibilidade à asma. Os genes localizados no cromossomo 5q (ADRB2, IL13 e IL4 e o gene ORMDL3, no cromossomo 17, identificado recentemente, parecem ser determinantes para a asma infantil. O diagnóstico e a farmacogenética podem ser as primeiras implicações clínicas de estudos extensivos sobre a genética da asma.OBJECTIVE: To present the most important and recent results of studies on asthma genetics. These data may help general physicians understand the impact of genetics on this complex disorder and how genes and polymorphisms influence asthma and atopy

  17. Genetic similarity between coriander genotypes using ISSR markers Similaridade genética entre genótipos de coentro por marcadores ISSR

    Directory of Open Access Journals (Sweden)

    Roberto de A Melo

    2011-12-01

    Full Text Available With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16, resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.Com o surgimento de novas cultivares, uma caracterização genética precisa é essencial, visando à utilização em programas de melhoramento ou para fins de registros e ou proteção de cultivares. Marcadores moleculares vêm complementando a caracterização morfológica e agronômica tradicional, uma vez que são virtualmente ilimitados, cobrem todo o genoma e não são influenciados pelo ambiente. A caracterização genética constitui a base para

  18. Variabilidad genética de la respuesta inflamatoria: I. Polimorfismo -511 C/T en el gen IL1β en diferentes subpoblaciones peruanas

    Directory of Open Access Journals (Sweden)

    Óscar Acosta

    2012-07-01

    Full Text Available El polimorfismo -511 citosina/timina (-511 C/T en la región promotora del gen interleuquina 1 beta (IL1β estα implicado en la producciσn diferencial de la citoquina y por tanto puede estar asociado a la respuesta inmuno-inflamatoria en obesidad, dislipidemias, cardiopatías, cáncer, infecciones, y el tratamiento con nutrientes y fármacos. Objetivos: Establecer la distribución de frecuencias de los genotipos y alelos del polimorfismo -511 C/T del gen IL1β en diferentes subpoblaciones peruanas. Diseño: Estudio descriptivo, observacional, transversal. Instituciones: Centro de Investigación de Bioquímica y Nutrición e Instituto de Medicina Tropical D.A. Carrión, Facultad de Medicina, UNMSM y Centro de Genética y Biología Molecular, Facultad de Medicina, USMP, Lima, Perú. Participantes: Pobladores peruanos. Intervenciones: Extracción de ADN genómico a partir de muestras sanguíneas o epitelio bucal según metodología estándar, de 168 individuos de 9 grupos subpoblacionales: 23 mestizos de Lima, 33 amazónicos (20 de Pucallpa y 13 de Amazonas y 112 andinos (12 de Ancash, 10 de Cajamarca, 18 de Huarochirí-Lima, 25 de Puno-Taquile, 25 de Puno-Uros y 22 de Puno-Anapia. Análisis del polimorfismo -511 C/T mediante la técnica de PCR/RFLP, con primers específicos y digestión con la enzima de restricción AvaI, detectándose los fragmentos por electroforesis en geles de agarosa al 2% y tinción con bromuro de etidio. Principales medidas de resultados: Frecuencias genotípicas y alélicas del gen IL1β. Resultados: Se encontró las siguientes frecuencias genotípicas CC=0,024; CT=0,369 y TT=0,607, consistentes con el equilibrio de Hardy-Weinberg; y las frecuencias alélicas fueron alelo C=0,208 y aleloT= 0,792. La frecuencia del alelo T, considerado el mutante, fue muy alta en los Uros de Puno (0.940 y más baja en los mestizos de Lima (0.609. La comparación de las frecuencias genotípicas (TT versus CT+CC y alélicas (T versus C

  19. Generic keys for the identification of larval Dytiscidae from Argentina (Coleoptera: Adephaga Claves genéricas para la identificación de las larvas de Dytiscidae de la Argentina (Coleoptera: Adephaga

    Directory of Open Access Journals (Sweden)

    Mariano C. Michat

    2008-12-01

    Full Text Available Generic keys for the identification of larvae of Dytiscidae from Argentina are presented. A total of 27 dytiscid genera are recognized in Argentina, included in 16 tribes and seven subfamilies. Twenty-two of the 27 genera are included in the keys. The remaining fi ve genera could not be included because their larvae are unknown (Bidessonotus Régimbart, Brachyvatus Zimmermann, Hemibidessus Zimmermann, Neobidessus Young or remain poorly known (Cybister Curtis. The keys are presented in English and Spanish. Morphological as well as chaetotaxic characters were included, and an emphasis was made to include easily seen and identifiable characters. However, due to the small size of many dytiscid larvae, some of the characters mentioned in the keys (especially the chaetotaxic ones are difficult to visualize through the observation with a stereoscopic microscope. Therefore, the use of a compound microscope for the identifi cation is recommended.Se presentan claves genéricas para la identificación de las larvas de Dytiscidae de la Argentina. Un total de 27 géneros de Dytiscidae son reconocidos en la Argentina, comprendidos en 16 tribus y siete subfamilias. Veintidós de los 27 géneros fueron incluidos en las claves. Los restantes cinco géneros no pudieron ser incluidos porque sus larvas son desconocidas (Bidessonotus Régimbart, Brachyvatus Zimmermann, Hemibidessus Zimmermann, Neobidessus Young o son conocidas muy imperfectamente (Cybister Curtis. Las claves se presentan en inglés y español. Se incluyeron caracteres morfológicos y de la quetotaxia, y se puso énfasis en incluir caracteres fácilmente visualizables e identificables. Sin embargo, debido al pequeño tamaño de muchas larvas de Dytiscidae, algunos de los caracteres mencionados en las claves (especialmente los de la quetotaxia son difíciles de visualizar a través de la observación con el microscopio estereoscópico. Por lo tanto, se recomienda el uso del microscopio compuesto para

  20. Targeted amplicon sequencing (TAS): a scalable next-gen approach to multilocus, multitaxa phylogenetics.

    Science.gov (United States)

    Bybee, Seth M; Bracken-Grissom, Heather; Haynes, Benjamin D; Hermansen, Russell A; Byers, Robert L; Clement, Mark J; Udall, Joshua A; Wilcox, Edward R; Crandall, Keith A

    2011-01-01

    Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples. Our TAS approach is easily scalable, simple in execution, neither time-nor labor-intensive, relatively inexpensive, and can be applied to a broad diversity of organisms and/or genes. Our TAS approach includes a bioinformatic application, BarcodeCrucher, to take raw next-gen sequence reads and perform quality control checks and convert the data into FASTA format organized by gene and sample, ready for phylogenetic analyses. We demonstrate our approach by sequencing targeted genes of known phylogenetic utility to estimate a phylogeny for the Pancrustacea. We generated data from 44 taxa using 68 different 10-bp multiplexing identifiers. The overall quality of data produced was robust and was informative for phylogeny estimation. The potential for this method to produce copious amounts of data from a single 454 plate (e.g., 325 taxa for 24 loci) significantly reduces sequencing expenses incurred from traditional Sanger sequencing. We further discuss the advantages and disadvantages of this method, while offering suggestions to enhance the approach.

  1. The political thought of Madame de Genlis: Rousseau’s Royalist Legacy

    Directory of Open Access Journals (Sweden)

    Carolina Armenteros

    2013-09-01

    Full Text Available On sait très peu sur la pensée politique de Félicité de Genlis (1746-1830 à part le fait qu’elle est devenue royaliste après la Révolution française. Cependant le tableau de l’Arcadie de Lagaraye dans Adèle et Théodore, ou lettres sur l’éducation (1782, fournit beaucoup de détails sur ce sujet. La figure du Législateur, en particulier, y démontre que la pensée politique de Genlis s’intéresse principalement à l’administration émotive des sociétés politiques, surtout en ce qui regarde les femmes, tout en rappelant – et en critiquant – les préoccupations politiques de Jean-Jacques Rousseau. Cet article analyse les correspondances et les contrastes entre Lagaraye et l’Arcadie de Clarens que Rousseau a dépeint dans Julie, ou la nouvelle Héloïse (1762. Il met Genlis en avant comme une critique sévère des idées de Rousseau, ainsi qu’une adhérente secrète du rousseauisme politique qui a développé sa vision politique sur la base de celle du Citoyen de Genève.

  2. Molecular evolution of two lineages related to influenza B virus based on HA1 gen

    Institute of Scientific and Technical Information of China (English)

    金青青

    2013-01-01

    Objective To study the evolutionary characteristics and rules of two lineages on influenza B virus.Methods A total of 126 HA1 sequences of strains isolated during 1940 to 201 2were downloaded from the GenBank.Time of the most recent common

  3. [Morphological and histological studies on the Chinese drug shan-dou-gen].

    Science.gov (United States)

    Fang, L Q; Guo, J X

    1992-01-01

    The Chinese drug "Shan-Dou-Gen" has been used for removing toxic heat, promoting the subsidence of swelling and soothing the sore throat since the ancient time. The authors made a survey of the drug "Shan-Dou-Gen" available in drug markets as well as the plant origin from the drug producing districts. The results showed that the drug "Shan-Dou-Gen" used in different regions in China at present are the roots or rhizomes derived from 9 species: Sophora tonkinensis Gagnep. (Leguminosae), Menispermum dauricum DC. (Menispermaceae), Indigofera amblyantha Craib (Leguminosae), I. carlesii Carib, I., fortunei Craib, I. decoa Lindl. var. ichangensis Y. Y. Fang et C. Z. Zheng, I. kirilowii Maxim. et Palibin, I. potaninii Craib, and Beesia calthaefolia (Maxim.) Ulbr. (Ranuculaceae). In this paper, the morphological characters of the crude drugs are described, compared and illustrated with photographs. The histological structures of the used parts are described, compared and illustrated with line drawings. The morphological and histological similarities and differences found among the above 9 species are summarized, and the key for the identification of the crude drugs is provided. As the drug "Shan-Dou-Gen" derived from different species has different actions and dosages, it is necessary to give different names to different species and use them correctly.

  4. Gen Y Recruitment: Understanding Graduate Intentions to Join an Organisation Using the Theory of Planned Behaviour

    Science.gov (United States)

    Warmerdam, Amanda; Lewis, Ioni; Banks, Tamara

    2015-01-01

    Purpose: Using the Theory of Planned Behaviour (TPB) framework, the purpose of this paper is to explore whether the standard TPB constructs explained variance in Generation Y (Gen Y) individuals' intentions to join their ideal organisation. Design/methodology/approach: A mixed methods approach was used featuring qualitative and quantitative…

  5. A genética das epilepsias The genetics of epilepsies

    National Research Council Canada - National Science Library

    Iscia Lopes-Cendes

    2008-01-01

    .... Mais recentemente, estudos de genética molecular e estratégias de descoberta de genes foram usados para revelar os mecanismos moleculares e celulares envolvidas em diversas síndromes epilépticas mendelianas. SÍNTESE DOS DADOS...

  6. Pacaella massiliensis gen. nov., sp. nov., a new bacterial species isolated from the human gut

    Directory of Open Access Journals (Sweden)

    S. Ndongo

    2017-03-01

    Full Text Available Herein, we report the main characteristics of a new species named Pacaella massiliensis gen. nov., sp. nov., strain Marseille-P2670T (CSUR P2670 that was isolated from the gut microbiota of a 45-year-old French patient.

  7. Au-delà du moralisme : Madame de Genlis et l’hospitalité

    Directory of Open Access Journals (Sweden)

    Sophie Bourgault

    2013-09-01

    Full Text Available Très peu de philosophes politiques se sont intéressés à l’oeuvre de Genlis, en partie parce que son oeuvre est communément réduite à un moralisme religieux insipide. Si Alice Laborde con-clut sa biographie en soulignant que Genlis « est moraliste avant tout », les travaux récents de Mary Trouille mettent tout autant l’accent sur le fait que la comtesse est « moralist to the core ». La visée du présent article est de nuancer cette lecture, non pas en niant le moralisme de Genlis, mais plutôt en montrant que celui-ci ne l’a pas empêchée de publier des textes à portée politique qui mériteraient notre attention aujourd’hui—particulièrement l’attention de ceux et celles qui se donnent pour tâche de penser l’accueil et le soin de l’Autre. A partir du Discours sur la sup-pression des convens et du Discours sur le luxe et l’hospitalité, nous indiquons non seulement l’actualité, mais aussi la surprenante radicalité des textes publiés par Genlis en période révolu-tionnaire.

  8. GWAS in a box: statistical and visual analytics of structured associations via GenAMap.

    Science.gov (United States)

    Xing, Eric P; Curtis, Ross E; Schoenherr, Georg; Lee, Seunghak; Yin, Junming; Puniyani, Kriti; Wu, Wei; Kinnaird, Peter

    2014-01-01

    With the continuous improvement in genotyping and molecular phenotyping technology and the decreasing typing cost, it is expected that in a few years, more and more clinical studies of complex diseases will recruit thousands of individuals for pan-omic genetic association analyses. Hence, there is a great need for algorithms and software tools that could scale up to the whole omic level, integrate different omic data, leverage rich structure information, and be easily accessible to non-technical users. We present GenAMap, an interactive analytics software platform that 1) automates the execution of principled machine learning methods that detect genome- and phenome-wide associations among genotypes, gene expression data, and clinical or other macroscopic traits, and 2) provides new visualization tools specifically designed to aid in the exploration of association mapping results. Algorithmically, GenAMap is based on a new paradigm for GWAS and PheWAS analysis, termed structured association mapping, which leverages various structures in the omic data. We demonstrate the function of GenAMap via a case study of the Brem and Kruglyak yeast dataset, and then apply it on a comprehensive eQTL analysis of the NIH heterogeneous stock mice dataset and report some interesting findings. GenAMap is available from http://sailing.cs.cmu.edu/genamap.

  9. Bornean freshwater crabs of the genus Arachnothelphusa gen. nov. (Crustacea: Decapoda: Brachyura: Gecarcinucidae)

    NARCIS (Netherlands)

    Ng, P.K.L.

    1991-01-01

    A new genus, Arachnothelphusa gen. nov., is established for five species of Bornean freshwater crabs (Gecarcinucidae) previously placed in Thelphusula Bott, 1969. One species from Sabah, A. terrapes, is here described as new. The genus is characterised by its long ambulatory legs, shape of the carap

  10. Qasimia gen. nov., an early Marattia-like fern from the Permian of Saudi Arabia

    NARCIS (Netherlands)

    Hill, C.R.; Wagner, R.H.; El-Khayal, A. A.

    1985-01-01

    The marattialean fern Qasimia schyfsmae (Lemoigne) gen. et comb. nov. is described from the Late Permian plant bed at Unayzah in central Saudi Arabia. Although no organic matter is preserved, impregnation of the compressions by iron minerals at an early stage of diagenesis has partly mineralised the

  11. Working with Gen Y Teachers: Dealing with a Changing Teacher Workforce

    Science.gov (United States)

    Williamson, Ronald; Meyer-Looze, Catherine

    2010-01-01

    A significant change is coming to American classrooms. As Baby Boomer teachers retire and are replaced by members of Generation Y (born between 1977 and 1995) there is growing evidence that they come from a far different set of experiences, experiences that will shape their lives and their careers (Coggins, 2008). Members of Gen Y share several…

  12. ‘Woes en Leeg’ en ‘Duisternis’ in Gen. 1 (1

    Directory of Open Access Journals (Sweden)

    J. L. Helberg

    1967-06-01

    Full Text Available Die Ieser word vir besonderhede en vir die verskillende beskouinge oor Gen. I verwys na die werke van Du Toit, Kroeze, N. H. Ridderbos, Scheepers en (die artikel van E. J. Young, met hulle literatuurlyste, synde enkele van die nuutste werke oor die onderhawige saak.

  13. Revision of "Phyllobrotica" from Taiwan with description of Jolibrotica gen. n. (Coleoptera, Chrysomelidae, Galerucinae).

    Science.gov (United States)

    Lee, Chi-Feng; Bezděk, Jan

    2015-01-01

    All Taiwanese species formerly classified the genus Phyllobrotica Chevrolat, 1836 are revised. Jolibrotica Lee & Bezděk, gen. n., is described for Phyllobrotica sauteri (Chûjô, 1935) (Taiwan, China: Guangxi) and Phyllobrotica chujoi Kimoto, 1969 (Taiwan). Phyllobrotica shirozui Kimoto, 1969 is transferred to the genus Haplosomoides. All species are redescribed and their diagnostic characters illustrated.

  14. Characterization and design of the FutureGen 2.0 carbon storage site

    Energy Technology Data Exchange (ETDEWEB)

    Gilmore, Tyler; Bonneville, Alain; Sullivan, Charlotte; Kelley, Mark; Appriou, Delphine; Vermeul, Vince; White, Signe; Zhang, Fred; Bjornstad, Bruce; Cornet, Francois; Gerst, Jacqueline; Gupta, Neeraj; Hund, Gretchen; Horner, Jake; Last, George; Lanigan, Dave; Oostrom, Mart; McNeil, Caitlin; Moody, Mark; Rockhold, Mark; Elliott, Mike; Spane, Frank; Strickland, Chris; Swartz, Lucy; Thorne, Paul; Brown, Christopher; Hoffmann, Jeffrey; Humphreys, Kenneth

    2016-10-01

    The objective of the FutureGen 2.0 Project was to demonstrate, at the commercial scale, the technical feasibility of implementing carbon capture and storage (CCS) in a deep saline formation in Illinois, USA. Over approximately 5 years, the FutureGen Alliance conducted a detailed site-selection process and identified a site for carbon sequestration storage in Morgan County, Illinois. The storage site was fully characterized, including the collection of seismic data and the drilling and characterization of a stratigraphic borehole. The characterization data provided critical input for developing a site-specific conceptual model and subsequent numerical modeling simulations. The modeling simulations, coupled with the upstream designs of the pipeline and power plant supported the development of a detailed 90 percent design that included the injection wells and associated control and monitoring infrastructure. Collectively, all these data were used by the FutureGen Alliance to develop the required documentation to support the applications for four underground injection control (UIC) permits (one for each proposed well). In August 2014, the U.S. Environmental Protection Agency issued four, first-of-their-kind, Class VI UIC permits for carbon sequestration in the United States to the FutureGen Alliance. The information and data generated under this project have been made publically available through reports and publications, including this journal and others.

  15. GWAS in a box: statistical and visual analytics of structured associations via GenAMap.

    Directory of Open Access Journals (Sweden)

    Eric P Xing

    Full Text Available With the continuous improvement in genotyping and molecular phenotyping technology and the decreasing typing cost, it is expected that in a few years, more and more clinical studies of complex diseases will recruit thousands of individuals for pan-omic genetic association analyses. Hence, there is a great need for algorithms and software tools that could scale up to the whole omic level, integrate different omic data, leverage rich structure information, and be easily accessible to non-technical users. We present GenAMap, an interactive analytics software platform that 1 automates the execution of principled machine learning methods that detect genome- and phenome-wide associations among genotypes, gene expression data, and clinical or other macroscopic traits, and 2 provides new visualization tools specifically designed to aid in the exploration of association mapping results. Algorithmically, GenAMap is based on a new paradigm for GWAS and PheWAS analysis, termed structured association mapping, which leverages various structures in the omic data. We demonstrate the function of GenAMap via a case study of the Brem and Kruglyak yeast dataset, and then apply it on a comprehensive eQTL analysis of the NIH heterogeneous stock mice dataset and report some interesting findings. GenAMap is available from http://sailing.cs.cmu.edu/genamap.

  16. Visualizing and Sharing Results in Bioinformatics Projects: GBrowse and GenBank Exports

    Science.gov (United States)

    Effective tools for presenting and sharing data are necessary for collaborative projects, typical for bioinformatics. In order to facilitate sharing our data with other genomics, molecular biology, and bioinformatics researchers, we have developed software to export our data to GenBank and combined ...

  17. Acantholachesilla gen. n. (Psocodea:'Psocoptera': Lachesillidae: Eolachesillinae: Graphocaeciliini) from Valle del Cauca, Colombia.

    Science.gov (United States)

    Aldrete, Alfonso N García; Manchola, Oscar Fernando Saenz; Obando, Ranulfo González

    2014-06-25

    Acantholachesilla saltoensis gen. et sp. n. is described from Valle del Cauca, Colombia, in the Lachesillidae, tribe Graphocaeciliini (Eolachesillinae). The genus is related to Dagualachesilla and Dagualachesilloides that occur in the same area, differing from them in the male clunial projection, phallosome, and female subgenital plate and gonapophyses.

  18. Riesgos sobre la salud de los alimentos modificados genéticamente: una revision bibliografica

    Directory of Open Access Journals (Sweden)

    José L. Domingo Roig

    2000-01-01

    Full Text Available A lo largo de 1999, se ha venido intensificando el debate sobre la seguridad de los alimentos modificados genéticamente, una importante y compleja área de investigación científica, la cual demanda unos estándares rigurosos. Diversos grupos, incluyendo asociaciones de consumidores y Organizaciones no Gubernamentales (ONGs han sugerido que todos los alimentos modificados genéticamente deberían ser sometidos a estudios a largo plazo con animales antes de su aprobación para el consumo humano. El principal objetivo de la presente revisión ha sido conocer cual es el estado actual de la cuestión en lo referente a los potenciales efectos adversos sobre la salud de los alimentos modificados genéticamente. Dos bases de datos, Medline y Toxline, así como una serie de direcciones de internet, han sido empleadas para la obtención de bibliografía. Aunque son numerosos los comentarios, noticias generales y cartas al Editor aparecidos en prestigiosas revistas, los artículos referentes a estudios experimentales sobre la seguridad de los alimentos modificados genéticamente son, sorprendentemente, muy escasos. Si se han obtenido resultados procedentes de la evaluación toxicológica de estos alimentos, no han sido publicados en revistas científicas y, por lo tanto, no han podido ser debidamente juzgados o contrastados.

  19. Pteroxena papillifera n. gen., n. sp., an endoparasitic organism (Copepoda?) from the gymnosomatous pteropod, Notobranchaea

    NARCIS (Netherlands)

    Stock, J.H.; Spoel, van der S.

    1976-01-01

    A single specimen of a strongly transformed, endoparasitic organism was found on the gymnosomatous pteropod Notobranchaea macdonaldi Pelseneer morpha pelseneeri Pruvot-Fol off Delaware Bay in the north-western Atlantic Basin. The parasite is described as Pteroxena papillifera n. gen., n. sp., and is

  20. GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism.

    Science.gov (United States)

    Chan, Ying Wai; West, Stephen

    2015-12-15

    Holliday junctions (HJs) that physically link sister chromatids or homologous chromosomes are formed as intermediates during DNA repair by homologous recombination. Persistent recombination intermediates are acted upon by structure-selective endonucleases that are required for proper chromosome segregation at mitosis. Here, we have purified full-length human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is analogous to that exhibited by the prototypic HJ resolvase E. coli RuvC. We find that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordinated incisions that lead to the formation of ligatable nicked duplex products. As observed with RuvC, cleavage of the first strand is rate limiting, while second strand cleavage is rapid. In contrast to RuvC, however, GEN1 is largely monomeric in solution, but dimerizes on the HJ. Using HJs containing non-cleavable phosphorothioate-containing linkages in one strand, we show that the two incisions can be uncoupled and that the first nick occurs upon GEN1 dimerization at the junction. These results indicate that the mechanism of HJ resolution is largely conserved from bacteria to man, despite a lack of sequence homology between the resolvases. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  1. Bases genéticas de la formación de fisuras labiales

    OpenAIRE

    Escobar, Lina María; Prada-Arismendy, Jeanette; Téllez, Carolina; Castellanos, Jaime

    2013-01-01

    El objetivo de este artículo, es revisar la información actualizada sobre la genética de la hendidura labio palatina y los genes reportados que pueden contribuir al desarrollo de esta compleja patología.

  2. Leucemia linfoblástica aguda infantil: una aproximación genómica

    Directory of Open Access Journals (Sweden)

    Silvia Jiménez-Morales

    2017-01-01

    En esta revisión se proporciona una visión global de la genómica de la LLA, describiendo algunas estrategias que contribuyen a la identificación de biomarcadores con potencial utilidad en la práctica clínica.

  3. Saying Hello World with GrGen.NET - A Solution to the TTC 2011 Instructive Case

    Directory of Open Access Journals (Sweden)

    Sebastian Buchwald

    2011-11-01

    Full Text Available We introduce the graph transformation tool GrGen.NET (www.grgen.net by solving the Hello World Case of the Transformation Tool Contest 2011 which consists of a collection of small transformation tasks; for each task a section is given explaining our implementation.

  4. A critical appraisal of ATLAS9 and NextGen 5 model atmospheres

    CERN Document Server

    Bertone, E; Chavez, M; Rodríguez, L H

    2001-01-01

    The fitting atmosphere parameters (Teff, g, and [Fe/H]) for over 300 stars in the Gunn & Striker and Jacoby et al. catalogs have been obtained relying on the Kurucz (1992) ATLAS9 and Hauschildt et al (1999) NextGen5 synthesis models. The output results are compared, and a critical appraisal of both theoretical codes is performed.

  5. When Opportunity Only Knocks Once: Lessons From Gen Petraeus In Iraq And Afghanistan

    Science.gov (United States)

    2016-03-01

    GEN McChrystal as well as Petraeus for their flattening this special operations organization and making it more lethal across multiple theaters.17...U.S. face and a necessary unity of effort for from the U.S. to help recover Iraq from its civil war. This teamwork was especially helpful to reduce

  6. pGenN, a gene normalization tool for plant genes and proteins in scientific literature.

    Directory of Open Access Journals (Sweden)

    Ruoyao Ding

    Full Text Available Automatically detecting gene/protein names in the literature and connecting them to databases records, also known as gene normalization, provides a means to structure the information buried in free-text literature. Gene normalization is critical for improving the coverage of annotation in the databases, and is an essential component of many text mining systems and database curation pipelines.In this manuscript, we describe a gene normalization system specifically tailored for plant species, called pGenN (pivot-based Gene Normalization. The system consists of three steps: dictionary-based gene mention detection, species assignment, and intra species normalization. We have developed new heuristics to improve each of these phases.We evaluated the performance of pGenN on an in-house expertly annotated corpus consisting of 104 plant relevant abstracts. Our system achieved an F-value of 88.9% (Precision 90.9% and Recall 87.2% on this corpus, outperforming state-of-art systems presented in BioCreative III. We have processed over 440,000 plant-related Medline abstracts using pGenN. The gene normalization results are stored in a local database for direct query from the pGenN web interface (proteininformationresource.org/pgenn/. The annotated literature corpus is also publicly available through the PIR text mining portal (proteininformationresource.org/iprolink/.

  7. Macrostylopyga gen. nov., a new genus of cockroaches (Dictyoptera: Blattidae), with descriptions of two new species.

    Science.gov (United States)

    Anisyutkin, Leonid N; Anichkin, Alexandr E; Thinh, Nguyen Van

    2013-01-01

    The genus Macrostylopyga gen. nov. and two species (M. grandis sp. nov. and M. bidupi sp. nov.) are described. A detailed morphological description with special attention to the male genitalic structures is provided. Some aspects of the evolution of wingless cockroaches are briefly discussed.

  8. Gen Y Recruitment: Understanding Graduate Intentions to Join an Organisation Using the Theory of Planned Behaviour

    Science.gov (United States)

    Warmerdam, Amanda; Lewis, Ioni; Banks, Tamara

    2015-01-01

    Purpose: Using the Theory of Planned Behaviour (TPB) framework, the purpose of this paper is to explore whether the standard TPB constructs explained variance in Generation Y (Gen Y) individuals' intentions to join their ideal organisation. Design/methodology/approach: A mixed methods approach was used featuring qualitative and quantitative…

  9. Karksilepis parva gen. et sp. nov. (Chondrichthyes from the Burtnieki Regional Stage, Middle Devonian of Estonia

    Directory of Open Access Journals (Sweden)

    Kleesment, Anne

    2008-12-01

    Full Text Available Dermal scales of a new chondrichthyan were discovered in four levels in sandstones of the Härma Beds (lower part of the Burtnieki Regional Stage, Middle Devonian in the Karksi outcrop of South Estonia. The classification of Karksilepis parva gen. et sp. nov. Märss is still uncertain.

  10. Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA

    Directory of Open Access Journals (Sweden)

    Yijin Liu

    2015-12-01

    Full Text Available We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction.

  11. Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.

    Science.gov (United States)

    Reinhold-Hurek, B; Hurek, T

    2000-03-01

    The taxonomic structure of members of the genus Azoarcus sensu lato was reassessed in a polyphasic approach. Two species, Azoarcus communis and Azoarcus indigens, three unnamed species containing diazotrophs associated with Kallar grass roots (groups C, D) and a group of strains (E) isolated from fungi were analysed. They were compared by PAGE analyses of cellular proteins, genomic fingerprints, morphological and nutritional features to new isolates from rice roots. All strains within groups C, D and E containing 5-12 isolates showed group-specific cell and colony morphology and carbon source utilization patterns, with exception of the obligately microaerobic strain BS20-3, a member of group C. All strains, with this exception, also had almost indistinguishable electrophoretic protein patterns and genomic fingerprints generated with tDNA-directed primers, suggesting they belong to the same species. Phylogenetic analyses of almost complete 16S rDNA sequences carried out with three different algorithms (neighbour-joining, maximum-likelihood, parsimony) revealed that Azoarcus sensu lato is not monophyletic. Groups C, D and E formed three distinct lineages located between the Azoarcus/Thauera and the Rhodocyclus clusters. Phylogenetic distances between groups C, D and E were as large as between other genera (93-94% sequence similarity). This suggested they have the rank of three different genera. Since it was possible to differentiate them from each other and other related bacteria by phenotypic features, three new genera with one type species each are proposed: Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.

  12. Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.

    Science.gov (United States)

    Hamada, Moriyuki; Iino, Takao; Iwami, Takahiro; Harayama, Shigeaki; Tamura, Tomohiko; Suzuki, Ken-ichiro

    2010-01-01

    Two Gram-positive bacteria, designated strains Aji5-31(T) and Ngc37-23(T), were isolated from the intestinal tracts of fishes. 16S rRNA gene sequence analysis indicated that both strains were related to the members of the family Dermatophilaceae, with 95.6-96.9% 16S rRNA gene sequence similarities. The family Dermatophilaceae contains 2 genera and 3 species: Dermatophilus congolensis, Dermatophilus chelonae and Kineosphaera limosa. However, it has been suggested that the taxonomic position of D. chelonae should be reinvestigated using a polyphasic approach, because the chemotaxonomic characteristics are not known (Stackebrandt, 2006; Stackebrandt and Schumann, 2000). Our present study revealed that strains Aji5-31(T), Ngc37-23(T) and D. chelonae NBRC 105200(T) should be separated from the other members of the family Dermatophilaceae on the basis of the following characteristics: the predominant menaquinone of strain Aji5-31(T) is MK-8(H(2)), strain Ngc37-23(T) possesses iso- branched fatty acids as major components, and the menaquinone composition of D. chelonae is MK-8(H(4)), MK-8 and MK-8(H(2)) (5 : 3 : 2, respectively). On the basis of these distinctive phenotypic characteristics and phylogenetic analysis results, it is proposed that strains Aji5-31(T) and Ngc37-23(T) be classified as two novel genera and species of the family Dermatophilaceae. The names are Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., and the type strains are Aji5-31(T) (=NBRC 104925(T) =DSM 22762(T)) and Ngc37-23(T) (=NBRC 104926(T) =DSM 22761(T)), respectively. In addition, D. chelonae should be reassigned to a new genus of the family Dermatophilaceae with the name Austwickia chelonae gen. nov., comb. nov.

  13. Diversidad genética en poblaciones humanas de dos regiones colombianas

    Directory of Open Access Journals (Sweden)

    Fernando Rondón

    2008-06-01

    Full Text Available Introducción: Estudios preliminares han mostrado la existencia de relaciones genéticas entre las poblaciones humanas del sur-occidente y las de la región andina colombiana, teniendo esto implicaciones en el grado de miscegenación de estas comunidades. No obstante el reconocimiento de este amplio proceso de mestizaje, no se tiene suficiente información que permita establecer la estructura y el grado de diversidad genética para cada región en particular y de la población colombiana en general. Objetivo: Determinar la estructura y diversidad genética presente en grupos poblacionales del centro y sur-occidente colombiano.Metodología: Se analizaron las frecuencias alélicas de 12 sistemas de microsatélites autosómicos y el tipo y frecuencia de RFLP’s de mtDNA presentes en 472 individuos de tres grupos étnicos: mestizos, indígenas y afroamericanos.Resultados: La caracterización de haplotipos de mtDNA en individuos afrodescendientes presentó 15% de marcadores típicos amerindios y 43% de africanos. El análisis de la diversidad genética mostró un índice de 0.72 en individuos Pijaos, valor cercano al índice de diversidad de la población mestiza de Cali (0.75. El análisis molecular de varianza (AMOVA a partir de los 12 STR’s, mostró que la estructuración genética no es significativa (FST de 0.032; adicionalmente se evidenció alta endogamia en la muestra mestiza de Caldas (0.43 y en la muestra indígena Coyaima (0.34.Conclusiones: Con los marcadores moleculares estudiados se estableció la estructura genética de poblaciones del sur-occidente colombiano confirmándose adicionalmente el grado de miscegenación y el flujo genético ocurrido entre diferentes grupos étnicos del centro y sur-occidente colombiano.

  14. Factores genéticos que inciden en la resistencia a enfermedades infecciosas en salmónidos y su aplicación en programas de mejoramiento Genetic factors involved in resistance to infectious diseases in salmonids and their application in breeding programmes

    Directory of Open Access Journals (Sweden)

    JM Yáñez

    2010-01-01

    Full Text Available El control de las enfermedades infecciosas es fundamental en el éxito del cultivo del salmón. El mejoramiento genético de la resistencia a enfermedades puede otorgar una opción factible y sustentable para el control de éstas. La Selección Asistida por Marcadores Moleculares (MAS o Genes (GAS se proyecta como una valiosa alternativa al mejoramiento convencional de la resistencia. Sin embargo, para implementar esta metodología es necesario el conocimiento previo de los factores genéticos involucrados en el carácter. En este trabajo se revisan y se discuten los aspectos más relevantes de la resistencia genética a enfermedades infecciosas en salmónidos y su aplicabilidad a programas de mejoramiento. En primer lugar, se presentan brevemente las enfermedades infecciosas más relevantes a nivel nacional. Además, se incluyen aspectos relacionados con el mejoramiento convencional para este rasgo cuantitativo, tales como criterios de selección, variación genética de la resistencia y correlaciones genéticas con otros caracteres de interés productivo. Por otra parte, se revisan tres aproximaciones moleculares utilizadas en la identificación de los factores genéticos involucrados en la resistencia: genes candidatos, con especial énfasis en el complejo mayor de histocompatibilidad (MHC o genes MH, detección de loci de efecto cuantitativo (QTL y estudios de expresión génica. Finalmente, se revisa y se discute en relación a la utilización de esta información molecular en la implementación de programas de mejoramiento genético que incluyan la resistencia a enfermedades infecciosas dentro de su objetivo de selección.The control of infectious diseases is essential in the success of salmon aquaculture. Genetic improvement of disease resistance may provide a feasible and sustainable option for the management of these diseases. Marker-assisted selection (MAS or Gene-assisted selection (GAS, represent a valuable alternative to the

  15. Programación de Horarios Escolares basados en Ritmos Cognitivos usando un Algoritmo Genético de Clasificación No-dominada, NSGA-II Scheduling of School Hours based on Cognitive Rhythms using a Non-dominated Sorting Genetic Algorithm, NSGA-II

    Directory of Open Access Journals (Sweden)

    Víctor F Suárez

    2013-01-01

    Full Text Available Se plantea la solución del problema de programación óptima de horarios escolares de una escuela pública Colombiana. Esto se hace considerando no solo la asignación adecuada de salones y docentes sino también los ritmos cognitivos que presentan los estudiantes como el factor más importante en el proceso de optimización. Se propone como método de solución el Algoritmo Genético de Clasificación No-dominada, NSGA-II. Los resultados muestran una mayor eficiencia del algoritmo en comparación con otros aplicados al mismo problema y evaluados en la misma forma. A nivel experimental, la metodología evidencia que los grupos programados mediante la orientación descrita presentan una reducción en los niveles de mortalidad académica en comparación con una programación horaria que no consideran los ritmos cognitivos de los estudiantesAbstract This paper proposes the implementation of an optimum academic agenda to solve the problem of scheduling a school day in Colombia. This is done by taking into account not only the appropriate assignment of rooms and professors but considering the cognitive rhythms of the students as the most relevant factor in the optimization. The Non-dominated Sorting Genetic Algorithm, NSGA-II is proposed as a solution method. The results show a higher efficiency of the algorithm when compared to other algorithms applied to the same problem and evaluated in the same form. At the experimental level the methodology shows a reduction in academic mortality rates in the groups where the methodology was implemented compared to groups that did not considered the cognitive rhythms of the students.

  16. Análisis genético del virus peruano de la fiebre amarilla

    Directory of Open Access Journals (Sweden)

    Carlos Yábar V

    2002-01-01

    Full Text Available Objetivo: Determinar las variantes genéticas de aislamientos del virus peruano de la Fiebre Amarilla (FA. Materiales y métodos: la región carboxiterminal del gen de la envoltura (E de cinco aislamientos de FA obtenidas de pacientes provenientes de Ayacucho 1978 (PER1, Junín 1995 (PER2, Cerro de Pasco (PER3, Cusco (1998 y San Martín (1999 fue amplificada por PCR, secuenciada y analizada con programas software de ADN. Resultados: el índice de similaridad de la secuencia de nucleótidos entre los cinco aislamientos reveló valores oscilantes entre 94,3% y 99,3%, mientras que la secuencia de aminoácidos presentó valores entre 97,6% y 99,7% de similaridad. El análisis filogenético demostró una distancia genética entre 0,40 y 6,50 mediante la secuencia de nucleótidos y a través de la secuencia de aminoácidos se observó un rango de 0,30 y 4,29. Sin embargo, las secuencias correspondientes a los sitios de glicosilación y a los epítopes de reconocimiento humoral fueron conservadas entre los cinco aislamientos, con excepción de algunos aislamientos de referencia reportados por otros autores. Conclusiones: los virus de FA peruanos forman un grupo filogenético distinto a otros virus de FA sudamericanos, basados en el análisis genéticos del gen E.

  17. Resgate vegetativo por alporquia de genótipos adultos de urucum (Bixa orellana L.

    Directory of Open Access Journals (Sweden)

    Nilton César Mantovani

    2010-09-01

    Full Text Available Este trabalho teve por objetivos avaliar a técnica de alporquia visando ao resgate vegetativo de genótipos de urucum (Bixa orellana L. e a obtenção de plantas fornecedoras de propágulos para processos de propagação clonal. Foram utilizadas dez plantas matrizes de urucum, com 12 anos de idade, obtidas partindo do cruzamento artificial entre os genótipos "Fruto Verde Piloso" X "Fruto Vermelho Liso". Os alporques foram realizados em ramos de 1 a 2 cm de diâmetro, utilizando-se como substrato uma mistura de vermiculita e musgo. Foi avaliado o efeito (1 do tipo de anelamento da casca dos ramos (total ou parcial, com 1 cm de comprimento, (2 do AIB (ácido indol-3-butírico a 0 e 4,92 mM aplicado em papel filtro e (3 do tipo de proteção dos alporques (filmes plásticos transparente ou preto ou tecido tencel, no enraizamento de alporque dos dez genótipos. A técnica de alporquia proporcionou o enraizamento de ramos dos dez genótipos avaliados, com eficiência variável de 20 a 100%, havendo efeito do genótipo sobre a frequência de enraizamento. A sobrevivência desses alporques foi de 100% após o plantio quando estes foram produzidos com anelamento total, tratados com AIB e protegidos com plástico transparente. Em casa de vegetação os alporques desenvolveram ramos partindo da brotação de gemas caulinares constituindo estoques de explantes apropriados para serem utilizados como estacas ou como fontes de segmentos nodais para a propagação in vitro desta espécie.

  18. Gen SRY y ausencia de tejido testicular en una mujer 47XYY con disgenesia gonadal.

    Directory of Open Access Journals (Sweden)

    William Jubiz

    2009-11-01

    Full Text Available Este artículo revisa conceptos actuales sobre determinación y diferenciación sexual con base en el estudio genético de una niña de 13 años que consultó por talla baja y aumento de peso. El examen físico mostró Tanner I en mamas y en vello púbico, sin signos de androgenización. Mientras el nivel de la hormona de crecimiento (GH era normal, las hormonas folículoestimulante (FSH y luteinizante (LH estaban aumentadas. Mediante laparoscopia y posterior estudio patológico se demostró la presencia de gonadas rudimentarias con ausencia de tejido testicular. Aunque el cariotipo obtenido fue 47XYY y el análisis molecular identificó la presencia del gen SRY, su funcionalidad es incierta, lo que hace necesaria su secuenciación, con la finalidad de determinar posibles mutaciones. En respuesta a la terapia con estrógenos y progesterona se desarrollaron tanto los caracteres sexuales secundarios como una menstruación normal. Aunque es posible que en la paciente haya una doble alteración genética donde concurran la mutación de novo de un gen y una no disyunción en la meiosis paterna, el caso descrito es ilustrativo de la importancia del estudio genético en la evaluación de la disgenesia gonadal.

  19. Genética molecular de caracteres cuantitativos en cruzamientos dialélicos de tomate

    Directory of Open Access Journals (Sweden)

    Guillermo Raúl Pratta

    2011-05-01

    Full Text Available El objetivo de este trabajo fue evaluar marcadores moleculares y caracteres cuantitativos en un cruzamiento dialélico completo sin recíprocos, entre cinco líneas recombinantes de tomate y sus híbridos. Se obtuvieron perfiles de AFLP ("amplified fragment length polymorphism" y de polipéptidos del pericarpio en cuatro estados de madurez del fruto de 15 genotipos. Se evaluaron, entre otros: peso, acidez titulable, pH, vida poscosecha y firmeza. Se calculó el porcentaje de polimorfismo para los marcadores moleculares y el porcentaje de variabilidad genética para los caracteres cuantitativos en el grupo de líneas recombinantes, el de híbridos y el conjunto de genotipos. Se realizaron análisis de agrupamiento con cada nivel de variación genética. Para AFLP, el porcentaje de polimorfismo varió entre 34 y 54% y, para los perfiles polipeptídicos, entre 40 y 78%. Mayor polimorfismo fue observado en el grupo de híbridos. La variabilidad genética fue de 100% para acidez y 34% para firmeza, con los mayores valores en los parentales. La similitud genética varió entre los genotipos según el nivel de variación genética; pero la consistencia en el agrupamiento de algunas líneas recombinantes y sus híbridos fue conservada, lo que evidenció asociaciones entre los datos moleculares y fenotípicos.

  20. Divergência genética entre clones de guaranazeiro

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    Nascimento Filho Firmino José do

    2001-01-01

    Full Text Available As técnicas multivariadas, para estimar a diversidade genética de um grupo de progenitores, têm sido utilizadas com freqüência pelos melhoristas de plantas. Os progenitores são utilizados em cruzamentos biparentais ou múltiplos, para formação de populações segregantes que tenham maior probabilidade de recuperação de genótipos superiores. Este trabalho foi realizado com o objetivo de identificar clones de guaranazeiro produtivos e divergentes que possam ser utilizados em um programa de cruzamentos para obter híbridos com alto valor heterótico e materiais para propagação vegetativa. Foram avaliados 148 clones de guaranazeiro atualmente em uso no programa de melhoramento genético da Embrapa-Centro de Pesquisa Agroflorestal da Amazônia Ocidental. Utilizou-se, para estimativa da divergência genética, a análise de agrupamento, em que a medida de dissimilaridade utilizada foi a distância euclidiana média padronizada e os métodos de agrupamento de otimização de Tocher e do vizinho mais próximo para construção do dendrograma entre grupos de clones. Houve a formação de sete grupos divergentes de clones. Concluiu-se que a divergência genética entre os clones não é grande, pois dois grupos foram formados com dois clones e três grupos foram formados somente com um único clone. Os clones CMU384 e CMU801 foram os mais próximos geneticamente e podem ser utilizados na formação de uma população com desenvolvimento vegetativo uniforme para uso em plantios comerciais.

  1. Transformação genética em espécies florestais.

    Directory of Open Access Journals (Sweden)

    Claudia Studart-Guimarães

    2010-08-01

    Full Text Available A transformação genética, que compreende a introdução de genes exógenos de forma controlada no genoma de uma célula vegetal e posterior regeneração da planta transgênica, tem contribuído com os programas de melhoramento genético de plantas pela obtenção de genótipos com novas características de interesse. O melhoramento de espécies florestais é limitado por características intrínsecas a tais espécies, como a altura dos indivíduos e o ciclo longo de vida. A transformação genética constitui, portanto, uma alternativa para a obtenção de espécies florestais com características desejáveis em um menor espaço de tempo. Plantas transgênicas com resistência a determinadas pragas, com melhor qualidade de madeira, maior produção de biomassa, tolerância a herbicidas, entre outras características de interesse, já foram obtidas para diferentes espécies florestais de importância econômica como álamo, eucalipto e pinheiros em geral. Este trabalho mostra a importância da transformação genética, associada a outras técnicas biotecnológicas no melhoramento de espécies florestais, as técnicas de transformação mais utilizadas e as características que já foram introduzidas nessas espécies pela transformação.

  2. EFEKTIVITAS TRANSFER DAN ANALISIS EKSPRESI GEN IMUNOGENIK TAHAN KOI HERPES VIRUS (KHV PADA IKAN MAS (Cyprinus carpio

    Directory of Open Access Journals (Sweden)

    Khairul Syahputra

    2014-04-01

    Full Text Available Penelitian transfer gen imunogenik tahan KHV (krt-GP11 pada ikan mas telah dilakukan dengan metode elektroporasi sperma menggunakan konsentrasi DNA yang berbeda. Penelitian ini bertujuan untuk mengetahui konsentrasi DNA optimal yang efektif digunakan dalam transfer gen pada ikan mas. Sperma dielektroporasi menggunakan tipe kejutan square wave dengan voltase 50 V dan jumlah kejutan tiga kali. Konsentrasi DNA yang digunakan adalah 10 μg/mL, 50 μg/mL, dan 100 μg/mL. Deteksi transgen pada sperma, embrio, dan larva dilakukan dengan metode PCR menggunakan primer spesifik untuk gen krt-GP11. Ekspresi transgen pada embrio dan larva dianalisis secara semi-kuantitatif dengan metode reverse transcriptase PCR (RT-PCR. Hasil penelitian menunjukkan bahwa gen krt-GP11 terdeteksi pada sperma, embrio, dan larva. Pemberian konsentrasi DNA 10 μg/mL lebih efektif digunakan dalam transfer gen krt-GP11 pada ikan mas, sedangkan peningkatan konsentrasi DNA yang digunakan tidak memberikan hasil yang berbeda terhadap keberhasilan transfer gen pada ikan mas. Ekspresi gen krt-GP11 yang berhasil diintroduksikan pada ikan mas baru dapat teramati dengan baik pada fase embrio.

  3. Estudios de la región 5'UTRTR del gen FMR-1 en pacientes con falla ovárica prematura Studies of the 5' - UTRTR region in the FMR-1 gene in patients withe Premature Ovarian Failure

    Directory of Open Access Journals (Sweden)

    V.A. Chiauzzi

    2010-12-01

    Full Text Available La falla ovárica prematura (FOP es un síndrome de patogénesis multicausal que afecta aproximadamente al 1% de las mujeres en edad reproductiva. Numerosos estudios asocian el estado de premutación (amplificación del número de tripletes CGG entre 50/55 y 200 repeticiones en el gen FMR-1 y FOP. Alrededor de un 4% de las pacientes FOP presentan alelos con premutación. La amplificación del número de tripletes por encima de 200 repeticiones causa el Síndrome de Fragilidad del X (SFX. El objetivo del presente trabajo fue estudiar la región 5´ no codificante del gen en un grupo de pacientes FOP de Argentina. La región de interés se amplificó por PCR a partir de muestras de ADN de 100 pacientes FOP y 145 mujeres controles. Los alelos de las pacientes y controles fueron agrupados en 7 categorías de acuerdo al número de tripletes obtenidos. Se observó que el número de repeticiones más frecuente se encuentra en el rango de 26 a 30 tripletes, tanto en pacientes como en controles. En el grupo de pacientes FOP, 5/197 (2.6% alelos no relacionados estudiados presentaron un número de tripletes CGG mayor a 50, mientras que sólo 1 de 290 (0.34% para el grupo control. Todas las pacientes FOP con valores de tripletes CGG mayor a 50 presentaron amenorrea secundaria. Estos resultados están en concordancia con lo comunicado para otras poblaciones acerca de la existencia de una asociación entre la premutación del gen FMR-1 y el desarrollo de FOP. Asimismo, los resultados obtenidos refuerzan la importancia de la genotipificación del gen FMR-1 en las pacientes FOP, a los efectos de estimar el riesgo de su descendencia para el SFX.Premature ovarian failure (POF is a syndrome of multicausal pathogenesis that affects 1% of women before the age of 40. Several studies associate the premutation state (CGG repeats increased in number between 50/55 and 200 in the FMR-1 gene and POF. About 4% of POF women have alleles in the FMR-1 gene in the permutation

  4. GenGIS 2: Geospatial Analysis of Traditional and Genetic Biodiversity, with New Gradient Algorithms and an Extensible Plugin Framework

    Science.gov (United States)

    Parks, Donovan H.; Mankowski, Timothy; Zangooei, Somayyeh; Porter, Michael S.; Armanini, David G.; Baird, Donald J.; Langille, Morgan G. I.; Beiko, Robert G.

    2013-01-01

    GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index) for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA), which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis. PMID:23922841

  5. GenGIS 2: geospatial analysis of traditional and genetic biodiversity, with new gradient algorithms and an extensible plugin framework.

    Directory of Open Access Journals (Sweden)

    Donovan H Parks

    Full Text Available GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA, which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis.

  6. Genética Molecular das Epidermólises Bolhosas Molecular Genetics of Epidermolysis Bullosa

    Directory of Open Access Journals (Sweden)

    Hiram Larangeira de Almeida Jr

    2002-10-01

    Full Text Available O estudo das alterações moleculares das epidermólises bolhosas tem contribuído para que se compreenda melhor essas enfermidades. Na epidermólise bolhosa simples a maioria dos casos está associada com alteração nas citoqueratinas basais 5 (gen KRT5 e 14 (gen KRT14, o que modifica o citoesqueleto na camada basal da epiderme, levando à degeneração dessa camada, formando bolha intra-epidérmica. Mutações na plectina (gen PLEC1, componente da placa interna do hemidesmossoma, levam também à clivagem intra-epidérmica. Na epidermólise bolhosa juncional vários gens estão envolvidos, em decorrência da complexidade da zona da membrana basal, todos levando ao descolamento dos queratinócitos basais na lâmina lúcida, pela disfunção da aderência entre esses e a lâmina densa. Alterações na laminina 5 (gens LAMA3, LAMB3 e LAMC2, integrina alfa6beta4 (gens ITGA6 e ITGB4 e colágeno XVII (gen COL17A1 foram descritas. Por fim, na epidermólise bolhosa distrófica apenas um gen está mutado, alterando o colágeno VII (gen COL7A1, principal componente das fibrilas ancorantes, produzindo clivagem abaixo da lâmina densa, variando fenotipicamente de acordo com a conseqüência da mutação. Outra aplicação importante dessas informações refere-se ao diagnóstico pré-natal, com a perspectiva no futuro da terapia gênica.New data regarding the molecular aspects of the heterogeneous group of epidermolysis bullosa has brought some important information about its pathogenesis. In epidermolysis bullosa simplex the majority of mutations are localized in the genes of the basal cytokeratin 5 (gene KRT5 and 14 (gene KRT14, cytolysis at this layer with intraepidermal blister is seen under light microscopy. Mutations of plectin (gene PLEC1, a protein found in the inner hemidesmosomal plaque, leads also to intraepidermal blisters. In junctional epidermolysis bullosa many proteins from the basal membrane zone are involved, such as laminin 5 (genes

  7. El uso de los tests genéticos por parte de las compañías de seguros

    OpenAIRE

    César Rodríguez Orgaz

    2011-01-01

    Se aborda la discusión en torno a la pertinencia del uso de tests genéticos por parte de las compañías de seguros. La idea que se defiende es la siguiente: si decidimos regular el recurso a los tests genéticos fuera del ámbito sanitario, que sea para hacer justicia a los derechos de terceras personas y en ningún caso por miedo a posibles discriminaciones. Se trata de llamar la atención sobre los riesgos aparejados al uso irresponsable de los datos genéticos por parte de las compañías de segur...

  8. Diversidad genética en Salvia elegans Vahl. (Lamiaceae), poblaciones con potencial para restauración

    OpenAIRE

    Bedolla García, Brenda Yudith

    2012-01-01

    El conocimiento de la estructura genética poblacional y diversidad genética de las especies es necesario para un buen manejo en conservación y/o restauración. Salvia elegans es una hierba leñosa que esta presente en bosques de coníferas distribuida a lo largo del Eje Neovolcánico. Se determinó la estructura genética de 8 poblaciones en S. elegans mediante la técnica de marcadores moleculares RAPD con 10 oligonucleótidos, que generaron 158 bandas, de las cuales el 95.5% fueron p...

  9. Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae, as Phaseolibacter flectens gen. nov., comb. nov.

    Science.gov (United States)

    Halpern, Malka; Fridman, Svetlana; Aizenberg-Gershtein, Yana; Izhaki, Ido

    2013-01-01

    Pseudomonas flectens Johnson 1956, a plant-pathogenic bacterium on the pods of the French bean, is no longer considered to be a member of the genus Pseudomonas sensu stricto. A polyphasic approach that included examination of phenotypic properties and phylogenetic analyses based on 16S rRNA, rpoB and atpD gene sequences supported the transfer of Pseudomonas flectens Johnson 1956 to a new genus in the family Enterobacteriaceae as Phaseolibacter flectens gen. nov., comb. nov. Two strains of Phaseolibacter flectens were studied (ATCC 12775(T) and LMG 2186); the strains shared 99.8 % sequence similarity in their 16S rRNA genes and the housekeeping gene sequences were identical. Strains of Phaseolibacter flectens shared 96.6 % or less 16S rRNA gene sequence similarity with members of different genera in the family Enterobacteriaceae and only 84.7 % sequence similarity with Pseudomonas aeruginosa LMG 1242(T), demonstrating that they are not related to the genus Pseudomonas. As Phaseolibacter flectens formed an independent phyletic lineage in all of the phylogenetic analyses, it could not be affiliated to any of the recognized genera within the family Enterobacteriaceae and therefore was assigned to a new genus. Cells were Gram-negative, straight rods, motile by means of one or two polar flagella, fermentative, facultative anaerobes, oxidase-negative and catalase-positive. Growth occurred in the presence of 0-60 % sucrose. The DNA G+C content of the type strain was 44.3 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, Pseudomonas flectens Johnson 1956 is transferred to the novel genus Phaseolibacter gen. nov. as Phaseolibacter flectens gen. nov., comb. nov. The type strain of Phaseolibacter flectens is ATCC 12775(T) = CFBP 3281(T) = ICMP 745(T) = LMG 2187(T) = NCPPB 539(T).

  10. Divergência genética entre cinco genótipos de melão rendilhado Genetic divergence among five muskmelon cultivars

    Directory of Open Access Journals (Sweden)

    Adriana Antonieta do Nascimento Rizzo

    2002-06-01

    Full Text Available Estimou-se a divergência genética entre cinco genótipos de melão rendilhado (Cucumis melo var. reticulatus Naud. (JAB-20, JAB-21, JAB-22, JAB-23 e 'Bônus nº 2' e determinou-se qual a contribuição relativa das 16 características avaliadas [nº médio de flores masculinas, hermafroditas/planta; produção total de frutos/m², peso médio dos frutos comerciáveis; diâmetro médio transversal e longitudinal do fruto (DMTF e DMLF; diâmetro médio transversal da inserção do pedúculo (DMTP; espessura média do mesocarpo e epicarpo (EMM e EME; diâmetro médio longitudinal e transversal do lóculo (DMTL e DMLL; proporção da cavidade (PC; desprendimento de sementes (DS; teor de sólidos solúveis totais (SST, pH e acidez titulável (AT] na divergência gen��tica. Obtiveram-se dois grupos de similaridade: I- JAB-20, JAB-21 e 'Bônus nº2' e II- JAB-22 e JAB-23. As características DMLF, DMTP, DMLL, DS e SST foram as que mais contribuíram para a divergência genética entre os genótipos.The genetic divergence of five cultivars of muskmelon was estimated (Cucumis melo var. reticulatus Naud (JAB-20, JAB-21, JAB-22, JAB-23 and 'Bônus nº2' and the relative contribution of each 16 characteristics were determined (number of male flowers per plant; total production of fruit, weight of fruits; longitudinal and transversal diameters of fruits; thickness and color of flesh and skin; longitudinal and transversal loculos diameter of fruits; seed loosing; netting thickness; and % total solvers solids, pH and total acidity in genetic divergence. Two groups of similarity were formed between the genitors by the values of D², one of then was constituted of the JAB-20 and JAB-21 and 'Bônus nº 2' genotypes, and another of the JAB-22 and JAB-23. The characteristics of longitudinal loculos diameters, longitudinal diameter of fruits, transversal diameter of peduncle insertion, % total solvers solids and seed loosing contributed to for genetic

  11. Genética y genómica de la hipertensión arterial: una actualización

    OpenAIRE

    Ramírez-Bello, Julián; Pérez-Méndez,Oscar; Ramírez-Fuentes,Silvestre; Carrillo-Sánchez,Silvia; Vargas-Alarcón, Gilberto; Fragoso, José Manuel

    2011-01-01

    La hipertensión arterial es considerada la principal causa de riesgo para el desarrollo de infarto agudo al miocardio, falla cardiaca, arritmia ventricular, nefropatía, ceguera, entre otras. La hipertensión arterial es una enfermedad multifactorial en la que participan factores ambientales, genéticos e intrínsecos como raza y género. La Organización Mundial de la Salud ha estimado que la prevalencia de la hipertensión se incrementará drásticamente, de modo que en la próxima década será la pri...

  12. Variabilidad genética de la respuesta inflamatoria. I. Polimorfismo -511 C/T en el gen IL1β en diferentes subpoblaciones peruanas

    OpenAIRE

    Óscar Acosta; Luis Solano; Doris Huerta; Daniel Oré; José Sandoval; José Figueroa; Ricardo Fujita

    2012-01-01

    Anales de la Facultad de Medicina es una publicación de la Universidad Nacional Mayor de San Marcos. El polimorfismo -511 citosina/timina (-511 C/T) en la región promotora del gen interleuquina 1 beta (IL1β) está implicado en la producción diferencial de la citoquina y por tanto puede estar asociado a la respuesta inmuno-inflamatoria en obesidad, dislipidemias, cardiopatías, cáncer, infecciones, y el tratamiento con nutrientes y fármacos. Objetivos: Establecer la distribución de frec...

  13. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  14. Low Genetic Diversity Among Garlic (Allium sativum L. Accessions Detected Using Random Amplified Polymorphic DNA (RAPD Escasa Diversidad Genética entre Accesiones de Ajo (Allium sativum L. Detectada Mediante ADN Polimórfico Amplificado al Azar (RAPD

    Directory of Open Access Journals (Sweden)

    Mario Paredes C

    2008-03-01

    Full Text Available Garlic (Allium sativum L. is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA. Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristicsEl ajo (Allium sativum L. es una especie de propagación vegetativa, que presenta una amplia variabilidad morfológica. Los clones de esta especie tienen una adaptación específica a diferentes regiones agroclimáticas. El objetivo de este estudio fue determinar la diversidad genética existente en 65 clones de ajos colectados en Chile e introducidos desde diferentes países, utilizando RAPD (ADN Polimórfico Amplificado al Azar. Para esta evaluación se utilizaron 40 partidores de 10-mers. Los partidores generaron entre dos y 20 bandas, observándose un alto número de patrones con bandas múltiples. Los fragmentos generados difieren en su tamaño entre 3.200 y 369 pb. Los partidores generaron 398 bandas, de las cuales un 87% fueron polimórficas. El análisis estadístico realizado detectó una similitud genética alta, de un 94% entre las accesiones evaluadas, donde aproximadamente un 70% de los clones formaron un grupo homogéneo. Sin embargo, este grupo incluye clones que presentan diferentes características agronómicas

  15. Class Schedule Assignment Based on Students Learning Rhythms Using A Genetic Algorithm Asignación de horarios de clase basado en los ritmos de aprendizaje de los estudiantes usando un algoritmo genético

    Directory of Open Access Journals (Sweden)

    Victor F. Suarez Chilma

    2013-03-01

    Full Text Available The objective of this proposal is to implement a school day agenda focused on the learning rhythms of students of elementary and secondary schools using a genetic algorithm. The methodology of this proposal takes into account legal requirements and constraints on the assignment of teachers and classrooms in public educational institutions in Colombia. In addition, this proposal provides a set of constraints focused on cognitive rhythms and subjects are scheduled at the most convenient times according to the area of knowledge. The genetic algorithm evolves through a process of mutation and selection and builds a total solution based on the best solutions for each group. Sixteen groups in a school are tested and the results of class schedule assignments are presented. The quality of the solution obtained through the established approach is validated by comparing the results to the solutions obtained using another algorithm.El objetivo de esta propuesta es implementar un horario escolar que tenga en cuenta los ritmos de aprendizaje en los estudiantes de educación primaria y secundaria, utilizando un algoritmo genético. La metodología considera los requerimientos legales y las restricciones necesarias para la asignación de maestros y aulas en instituciones educativas públicas de Colombia. Adicionalmente, se establecen un conjunto de restricciones relacionadas con el enfoque en los ritmos cognitivos, determinando las horas de la jornada en las que es más conveniente la ubicación de ciertas materias de acuerdo al área del conocimiento al que pertenecen. El algoritmo genético evoluciona mediante un proceso de mutación y selección, a través del cual se construye una solución completa a partir de la búsqueda de las mejores soluciones por grupo. Se presentan los resultados de las pruebas realizadas para la asignación de una institución con 16 grupos. La calidad de las soluciones obtenidas de acuerdo al enfoque establecido es validada

  16. Polimorfismos del gen BoLA-DRB3.2* en ganado criollo colombiano

    Directory of Open Access Journals (Sweden)

    Darwin Hernández H.

    2013-10-01

    Full Text Available Objetivo. Caracterizar el polimorfismo del gen BoLA-DRB3.2* en las razas bovinas criollas y colombianas. Materiales y métodos. En 360 muestras de ADN de ocho razas bovinas criollas (Blanco Orejinegro, Casanareño, Costeño con Cuernos, Chino Santandereano, Caqueteño, Hartón del Valle, Romosinuano y San Martinero, dos razas sintéticas Colombianas (Lucerna y Velásquez y dos razas foráneas (Brahman y Holstein se evaluó el polimorfismo del gen BoLA-DRB3.2 mediante técnicas moleculares (PCR-RFLP; se calculó el número promedio de alelos (NPA, las frecuencias, la heterocigocidad esperada (He y observada (Ho, el equilibrio de Hardy-Weinberg, la estructura genética y los valores de FST y FIS. Resultados. El NPA fue 14.6 ± 3.8 siendo Caqueteño la raza con mayor NPA (25 y el menor el Chino Santandereano (10. Se encontraron 41 alelos BoLA-DRB3.2* los más frecuentes fueron *28, *37, *24, *23, *20, *27, *8, *16, *39 (0.17, 0.11, 0.10, 0.09, 0.09, 0.07, 0.07 y 0.06 respectivamente. Se encontró alta diversidad genética (He = 0.878 con mayor valor en Caqueteño (0.96 y menor en San Martinero (0.81. Todas las razas se encontraron en equilibrio de Hardy-Weinberg, se encontraron valores altamente significativos de diferenciación genética (FST= 0.044 y de coeficiente de endogamia (FIS = 0.249. Conclusiones. El ganado criollo colombiano posee alto polimorfismo del gen BoLA-DRB3.2* representado en los altos valores de NPA y diversidad génetica.

  17. Cordiamyia globosa gen.n. e sp.n. (Diptera, Cecidomyiidae, Cecidomyiidi associado com Cordia Verbenacea DC. (Boraginaceae no Brasil Cordiamyia globosa gen.n. and sp.n. (Diptera, Cecidomyiidae associated with Cordia verbekacea DC. (Boraginaceae in Brazil

    Directory of Open Access Journals (Sweden)

    Valéria Cid Maia

    1996-01-01

    Full Text Available Cordiamyia globosa gen.n., sp.n. (Diptera, Cecidomyiidae, Cecidomyiidi associated with Cordia verbenacea (Boraginaceae, in Brazil, is described and illustrated (larva, pupa, male, female and gall.

  18. Genética comunitária : a inserção da genética médica na atenção primária à saúde em Porto Alegre

    OpenAIRE

    Taiane Alves Vieira

    2012-01-01

    Introdução: Com o melhor controle das causas ambientais, as doenças genéticas e as malformações congênitas cada vez mais ganham destaque como fatores morbidade e mortalidade, assim como cada vez mais se reconhece a importância da base genética para as doenças comuns. Desta forma, é necessário desenvolver estratégias de prevenção e controle destas condições, assim como acesso aos cuidados de saúde para pessoas com ou em risco de desenvolver uma doença genética. A integração da genética na Aten...

  19. GenBank数据库和PubMed数据库中序列数据信息检索比较%Comparison of sequential data retrieval results from GenBank and PubMed

    Institute of Scientific and Technical Information of China (English)

    李轶

    2009-01-01

    通过对GenBank数据库和PubMed数据库的数据来源、检索界面和检索结果等的对比分析,发现2个检索库检索的序列数据信息存在差别,GenBank数据库检索结果和检准率均高于PubMed数据库.

  20. Nitridogermanate nitrides Sr7[GeN4]N2 and Ca7[GeN4]N2: synthesis employing sodium melts, crystal structure, and density-functional theory calculations.

    Science.gov (United States)

    Junggeburth, Sebastian C; Oeckler, Oliver; Johrendt, Dirk; Schnick, Wolfgang

    2008-12-15

    The alkaline earth nitridogermanate nitrides AE(7)[GeN(4)]N(2) (AE = Ca, Sr) have been synthesized using a Na flux technique in sealed Ta tubes. According to single-crystal X-ray diffraction the isotypic compounds crystallize in space group Pbcn (No. 60) with Z = 4, (Sr(7)[GeN(4)]N(2): a = 1152.6(2), b = 658.66(13), c = 1383.6(3) pm, V = 1050.5(4) x 10(6) pm(3), R1 = 0.049; Ca(7)[GeN(4)]N(2): a = 1082.6(2), b = 619.40(12), c = 1312.1(3) pm, V = 879.8(3) x 10(6) pm(3), R1 = 0.016). Owing to the high N/Ge ratio, the compounds contain discrete N(3-) ions coordinated by six AE(2+) besides discrete [GeN(4)](8-) tetrahedrons. One of the AE(2+) ion is coordinated by only four N(3-) ions, which is rather an unusual low coordination number for Sr(2+). Together with the isolated [GeN(4)](8-) tetrahedrons, these Sr(2+) ions form chains of alternating cation centered edge sharing tetrahedrons. The electronic structure and chemical bonding in Sr(7)[GeN(4)]N(2) has been analyzed employing linear muffin-tin orbital (LMTO) band structure calculations.

  1. TopoGen: A Network Topology Generation Architecture with application to automating simulations of Software Defined Networks

    CERN Document Server

    Laurito, Andres; The ATLAS collaboration

    2017-01-01

    Simulation is an important tool to validate the performance impact of control decisions in Software Defined Networks (SDN). Yet, the manual modeling of complex topologies that may change often during a design process can be a tedious error-prone task. We present TopoGen, a general purpose architecture and tool for systematic translation and generation of network topologies. TopoGen can be used to generate network simulation models automatically by querying information available at diverse sources, notably SDN controllers. The DEVS modeling and simulation framework facilitates a systematic translation of structured knowledge about a network topology into a formal modular and hierarchical coupling of preexisting or new models of network entities (physical or logical). TopoGen can be flexibly extended with new parsers and generators to grow its scope of applicability. This permits to design arbitrary workflows of topology transformations. We tested TopoGen in a network engineering project for the ATLAS detector ...

  2. No quenching of magnetic moment for the GenCo (n=1-13) clusters: First-principles calculations

    Science.gov (United States)

    Jing, Qun; Tian, Fu-yang; Wang, Yuan-xu

    2008-03-01

    The authors predict that for the GenCo (n=1-13) clusters the magnetic moment does not quench, which is dark contrast to the previous results with transition-metal-doped Sin clusters. It may be due to the unpaired electrons of the Co atom in the clusters. For the ground state structures of the GenCo (n⩾9) clusters, the Co atom completely falls into the center of the Ge outer frame, forming metal-encapsulated Gen cages. The doping of the Co atom enhances the stability of the host Gen clusters. The Ge10Co cluster with the bicapped tetragonal antiprism structure is more stable than others, which agrees very well with the results of the experiment of the Co /Ge binary clusters by the laser vaporization.

  3. El uso de los tests genéticos por parte de las compañías de seguros

    Directory of Open Access Journals (Sweden)

    César Rodríguez Orgaz

    2011-01-01

    Full Text Available Se aborda la discusión en torno a la pertinencia del uso de tests genéticos por parte de las compañías de seguros. La idea que se defiende es la siguiente: si decidimos regular el recurso a los tests genéticos fuera del ámbito sanitario, que sea para hacer justicia a los derechos de terceras personas y en ningún caso por miedo a posibles discriminaciones. Se trata de llamar la atención sobre los riesgos aparejados al uso irresponsable de los datos genéticos por parte de las compañías de seguros, y se llega a la conclusión de que un uso tal compromete los derechos de terceras personas implicadas. Por último, plantearemos posibles escenarios para el futuro sobre el procesamiento de la información genética

  4. Squamasnema amazonica n. gen. n. sp. (Heligmonellinae): A new parasite of Proechimys roberti (Rodentia: Echimyidae) in the Brazilian Amazon.

    Science.gov (United States)

    Cordeiro, Helrik da Costa; Melo, Francisco Tiago de Vasconcelos; Furtado, Adriano Penha; Giese, Elane Guerreiro; Maldonado, Arnaldo; dos Santos, Jeannie Nascimento

    2015-08-01

    A new species of nematode, Squamasnema amazonica n. gen. n. sp., is described based on specimens found parasitizing the small intestine of Proechimys roberti (Rodentia: Echimyidae) collected during a survey of the fauna of Tapirapé-Aquirí National Forest (Brazil, Eastern Brazilian Amazon). The nematodes were fixed and processed for light microscopy and scanning electron microscopy (SEM). These nematodes were classified under the family Heligmonellidae and the subfamily Heligmonellinae. Although several species in the family Heligmonellidae exhibit discontinuous ridges, Squamasnema n. gen. and Trichotravassosia are the only genera with columns of scales along their entire body, as an apomorphy of the synlophe. Squamasnema n. gen. has columns of cuticular cells along its body, except for on the left flank, and exhibits a synlophe with no size gradient or inclination and does not present chitinized structures supporting the synlophe. Therefore, due to these morphological differences of Squamasnema n. gen., the creation of a new genus was necessary.

  5. Diversidad genética de Plasmodium falciparum y sus implicaciones en la epidemiología de la malaria

    National Research Council Canada - National Science Library

    Judy Natalia Jiménez; Carlos Enrique Muskus; Iván Darío Vélez

    2005-01-01

    La diversidad genética le confiere a Plasmodium falciparum la capacidad de evadir la respuesta inmune del hospedero y producir variantes resistentes a medicamentos y a vacunas, aspectos que juegan un papel importante...

  6. Desafios para a universalização da genética clínica: o caso brasileiro

    OpenAIRE

    2011-01-01

    Acesso restrito: Texto completo. p. 61-68 Este artigo aborda as dificuldades de inserir a genética médica como parte do Sistema Único de Saúde (SUS) no Brasil. Em 2009, foi instituída no Brasil a Política Nacional de Atenção Integral em Genética Médica, cujo pilar central seria o aconselhamento genético. Porém, são problemas estratégicos para a implementação dessa política a falta de programas de formação em aconselhamento genético, o desconhecimento acerca de quantos profissionais existem...

  7. Estudi comparatiu de l'estructura del gen "Adh" a vàries espècies de "Drosophila"

    OpenAIRE

    Marfany i Nadal, Gemma

    1991-01-01

    S'ha caracterizat l'estructura de la regió genòmica del gen "Adh" a quatre espècies del gènere "Drosophila", pertanyents al subgrup "obscura": "D. ambigua", "D. subobscura", "D. madeirensis" i "D. guanche" amb els objectius d'analitzar l'organització i l'evolució d'aquesta regió genòmica dins d'aquest gènere i clarificar les relacions filogenètiques d'aquestes espècies. Per assolir aquestes fites, es van construir llibreries genòmiques de cada espècie que es van crivellar amb una sonda que co...

  8. Simulation of an extended surface detector IceVeto for IceCube-Gen2

    Energy Technology Data Exchange (ETDEWEB)

    Hansmann, Tim; Auffenberg, Jan; Haack, Christian; Hansmann, Bengt; Kemp, Julian; Konietz, Richard; Leuner, Jakob; Raedel, Leif; Stahlberg, Martin; Schoenen, Sebastian; Wiebusch, Christopher [III. Physikalisches Institut B, RWTH Aachen University (Germany); Collaboration: IceCube-Collaboration

    2016-07-01

    IceCube is a neutrino observatory located at the geographic South Pole. The main backgrounds for IceCube's primary goal, the measurement of astrophysical neutrinos, are muons and neutrinos from cosmic-ray air showers in the Earth's atmosphere. Strong supression of these backgrounds from the Southern hemisphere has been demonstrated by coincident detection of these air showers with the IceTop surface detector. For an extended instrument, IceCube-Gen2, it is considered to build an enlarged surface array, IceVeto, that will improve the detection capabilities of coincident air showers. We will present simulation studies to estimate the IceVeto capabilities to optimize the IceCube-Gen2 design.

  9. Simulating GenCo bidding strategies in electricity markets with an agent-based model

    Energy Technology Data Exchange (ETDEWEB)

    Botterud, Audun; Thimmapuram, Prakash R.; Yamakado, Malo

    2005-12-15

    In this paper we use an agent-based simulation model, EMCAS, to analyze market power in electricity markets. We focus on the effect of congestion management on the ability of generating companies (GenCos) to raise prices beyond competitive levels. An 11-node test power system is used to compare a market design based on locational marginal pricing with a market design that uses system marginal pricing and congestion management by counter trading. Bidding strategies based on both physical and economic withholding are compared to a base case with production cost bidding. The results show that unilateral market power is exercised under both pricing mechanisms. However, the largest changes in consumer costs and GenCo profits due to strategic bidding occur under the locational marginal pricing scheme. The analysis also illustrates that agent-based modeling can contribute important insights into the complex interactions between the participants in transmission-constrained electricity markets. (Author)

  10. EL GEN PIG-A COMO INDICADOR DE MUTACIONES SOMÁTICAS In vivo

    OpenAIRE

    Mercedes Monserrat PACHECO MARTÍNEZ; Elsa CERVANTES RÍOS; María Del Carmen GARCÍA RODRÍGUEZ; Alda Rocío ORTIZ MUÑIZ

    2014-01-01

    La medición cuantitativa de las mutaciones es fundamental en el campo de la mutagé - nesis ambiental. Recientemente se han descrito nuevos métodos para su valoración y para la evaluación de mutaciones inducidas por agentes químicos in vivo . Uno de ellos está basado en el análisis del fenotipo relacionado con la expresión del gen fosfatidi - linositol glicano de clase A ( PIG-A en humanos y Pig-a en roedores). Este gen está ligado al cromosoma X y es esencial para la síntesis del ancla glicos...

  11. EFEKTIVITAS METODE TRANSFEKSI DALAM TRANSFER GEN PADA ZIGOT IKAN CUPANG ALAM (WILD BETTA, Betta imbellis

    Directory of Open Access Journals (Sweden)

    Anjang Bangun Prasetio

    2013-08-01

    dan 3:1 dengan jumlah ulangan masing-masing sebanyak enam kali. Sebagai kontrol, ditambahkan juga perlakuan non transfeksi (non transgenik yaitu tanpa penyisipan gen GFP maupun RFP. Pengamatan dilakukan sejak perkembangan zigot mulai dari penghitungan derajat penetasan (HR dan sintasan larva (SR. Hasil penelitian menunjukkan bahwa setelah dilakukan transfeksi tidak memperlihatkan pola yang jelas dari setiap perlakuan, namun secara umum tidak berbeda signifikan dengan kontrol non transgenik. PCR pada embrio dan larva menunjukkan hasil positif di mana DNA teramplifikasi pada ukuran sekitar 0,6 kb untuk beberapa ulangan. Dari hasil yang diperoleh ini dapat ditarik kesimpulan bahwa metode transfeksi efektif digunakan untuk transfer gen ikan cupang alam, wild betta (Betta imbellis.

  12. Gen 2.0 Mixer/Ejector Nozzle Test at LSAF June 1995 to July 1996

    Science.gov (United States)

    Arney, L. D.; Sandquist, D. L.; Forsyth, D. W.; Lidstone, G. L.; Long-Davis, Mary Jo (Technical Monitor)

    2005-01-01

    Testing of the HSCT Generation 2.0 nozzle model hardware was conducted at the Boeing Low Speed Aeroacoustic Facility, LSAF. Concurrent measurements of noise and thrust were made at critical takeoff design conditions for a variety of mixer/ejector model hardware. Design variables such as suppressor area ratio, mixer area ratio, liner type and thickness, ejector length, lobe penetration, and mixer chute shape were tested. Parallel testing was conducted at G.E.'s Cell 41 acoustic free jet facility to augment the LSAF test. The results from the Gen 2.0 testing are being used to help shape the current nozzle baseline configuration and guide the efforts in the upcoming Generation 2.5 and 3.0 nozzle tests. The Gen 2.0 results have been included in the total airplane system studies conducted at MDC and Boeing to provide updated noise and thrust performance estimates.

  13. INTRODUKSI GEN Sitrat Sintase KE DALAM RUMPUT LAUT Kappaphycus alvarezii MENGGUNAKAN Agrobacterium tumefaciens

    Directory of Open Access Journals (Sweden)

    Ristanti Frinra Daud

    2013-08-01

    ekonomis penting. Ice-ice merupakan penyakit yang paling umum menyerang rumput laut dan menyebabkan menurunnya produksi rumput laut. Penyakit ini disebabkan oleh perubahan salinitas, suhu, dan pencemaran logam berat. Asam sitrat digunakan sebagai pengkelat logam berat. Introduksi gen sitrat sintase ke dalam genom tanaman diketahui dapat mengurangi cekaman oksidatif. Penelitian ini bertujuan untuk mengintroduksi gen sitrat sintase ke dalam genom K. alvarezii menggunakan perantara Agrobacterium tumefaciens. Berdasarkan eksplan yang tahan pada media seleksi higromisin, efisiensi transformasi pada K. alvarezii sebesar 7,5%. Efisiensi regenerasi tunas transgenik putatif sebesar 100%, efisiensi tunas non transgenik sebesar 100%. Analisis molekular menggunakan teknik PCR, satu dari lima K. alvarezii transgenik putatif mengandung transgen PaCS di bawah kendali promoter 35S CaMV.

  14. Els gens zombis continuen la vida més enllà de la mort

    OpenAIRE

    Bueno i Torrens, David, 1965-

    2016-01-01

    Periòdicament, algunes sèries de televisió, el cinema o la literatura juvenil han posat de moda els zombis. Que ningú es confongui, els zombis són pura ficció i no tenen cap validesa científica. Però un estudi recent indica que, després de morir, a les cèl·lules del difunt s'activen alguns programes genètics que es mantenen en funcionament diversos dies després del decés. En aquest procés hi hauria més de mil gens implicats, segons els autors de l'estudi, un equip format per investigadors del...

  15. Internal combustion engine report: Spark ignited ICE GenSet optimization and novel concept development

    Energy Technology Data Exchange (ETDEWEB)

    Keller, J.; Blarigan, P. Van [Sandia National Labs., Livermore, CA (United States)

    1998-08-01

    In this manuscript the authors report on two projects each of which the goal is to produce cost effective hydrogen utilization technologies. These projects are: (1) the development of an electrical generation system using a conventional four-stroke spark-ignited internal combustion engine generator combination (SI-GenSet) optimized for maximum efficiency and minimum emissions, and (2) the development of a novel internal combustion engine concept. The SI-GenSet will be optimized to run on either hydrogen or hydrogen-blends. The novel concept seeks to develop an engine that optimizes the Otto cycle in a free piston configuration while minimizing all emissions. To this end the authors are developing a rapid combustion homogeneous charge compression ignition (HCCI) engine using a linear alternator for both power take-off and engine control. Targeted applications include stationary electrical power generation, stationary shaft power generation, hybrid vehicles, and nearly any other application now being accomplished with internal combustion engines.

  16. Transhumanistas y Bioconservadores en torno al dopaje genético

    Directory of Open Access Journals (Sweden)

    Raúl Francisco Sebastián Solanes

    2013-04-01

    Full Text Available En el presente texto proponemos una reflexión sobre uso de tecnologías genéticas que  aumentan el rendimiento deportivo como futuro campo de investigación de una Neuroética social. Este problema, que se ha dado en llamar “dopaje genético”, congrega a su alrededor un debate entre los partidarios del Bioconservadurismo y del Transhumanismo. Expondremos la concepción de dos importantes representantes del Transhumanismo (J. Savulescu y C. Tamburrini y de dos conocidos partidarios del Bioconservadurismo (M. Sandel y R. L. Simon, a fin de subrayar la importancia de este debate y las futuras implicaciones en la mejora del rendimiento físico, cognitivo y educacional a las que se deberá hacer frente desde el nivel socio-cultural de la Neuroética.

  17. Genética en humana en Estomatología

    OpenAIRE

    2014-01-01

    El creciente interés que están despertando las enfermedades genéticas dentro del contexto de la patología humana, se debe al espectacular avance que han experimentado las cada vez más complejas metodologías que con un número día a día mayor de científicos investigan los mecanismos de la herencia. Es así como novedosos estudios bioquímicos, cromosómicos y poblaciones, han permitido reconocer en muchas entidades de causalidad confusa hasta no hace mucho, una clara etiología genética. El crec...

  18. Comportamiento productivo de cerdos portadores del gen del halotano en condiciones medioambientales no controladas

    Directory of Open Access Journals (Sweden)

    Sánchez-Chiprés, David Román

    2008-05-01

    Full Text Available El síndrome de estrés porcino es una enfermedad genética que afecta acerdos de crecimiento rápido, manifestándose con muerte súbita durante las condiciones de manejo o al sacrificio, como carne pálida, suave y exudativa. Se econoce que el síndrome de estrés porcino (PSS, el síndrome de la carne pálida suave y exudativa (PSE así como la hipertermia maligna (MH, comparten una mutación en el gen receptor de la rianodina (RYR1. Un método de diagnóstico basado en DNA y el uso de la técnica de reacción en cadena de la polimerasa, permite hacer una distinción de los genotipos para el locus halotano.

  19. Frecuencia de algunas enfermedades genéticas en Neuropediatría

    OpenAIRE

    Tatiana Zaldívar Vaillant; Nicolás Garófalo Gómez; José Vargas Díaz; Edelsia Rojas Massipe; Lucía Margarita Novoa López; Vivian Bermúdez Linares; Ivonne Martín Hernández; Carlos Ignacio Viñas Portilla

    2012-01-01

    Introducción: las enfermedades neurológicas en Pediatría son diversas y obedecen a un gran número de causas: infecciosas, genéticas, metabólicas y degenerativas, entre otras. El diagnóstico genético, dentro del método clínico en Neurología, está relacionado con el diagnóstico etiológico. Existen muy pocas publicaciones que reflejen la frecuencia de las enfermedades neurogenéticas como grupo etiológico. Objetivo: describir la frecuencia de algunas enfermedades neuropediátricas en la Consulta d...

  20. Mucopolisacaridosis II: nueva mutación patogénica en gen IDS

    Directory of Open Access Journals (Sweden)

    Eugenia Pérez-Elizondo

    2014-12-01

    Full Text Available La mucopolisacaridosis tipo II es una enfermedad lisosomal producida por la deficiencia de la enzima iduronato 2 sulfatasa. Es una condición infrecuente de herencia recesiva ligada al X, que puede producir importante discapacidad progresiva. El análisis molecular es una técnica útil en la confirmación diagnóstica, que además permite detección de portadores asintomáticos, brindando la oportunidad de asesoría genética. Se presenta el caso de un paciente con mucopolisacaridosis tipo II, en quien se documentó una nueva mutación patogénica en el Gen IDS.

  1. Thermal stability study for candidate stainless steels of GEN IV reactors

    Energy Technology Data Exchange (ETDEWEB)

    Simeg Veternikova, J., E-mail: jana.veternikova@stuba.sk [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Degmova, J. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Pekarcikova, M. [Institute of Materials Science, Faculty of Materials Science and Technology, Slovak University of Technology, Paulinska 16, 917 24 Trnava (Slovakia); Simko, F. [Department of Molten Salts, Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 36 Bratislava (Slovakia); Petriska, M. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Skarba, M. [Slovak University of Technology, Vazovova 5, 812 43 Bratislava (Slovakia); Mikula, P. [Institute of Nuclear and Physical Engineering, Faculty of Electrical and Information Technology, Slovak University of Technology, Ilkovicova 3, 812 19 Bratislava (Slovakia); Pupala, M. [Department of Molten Salts, Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 36 Bratislava (Slovakia)

    2016-11-30

    Highlights: • Thermal resistance of advanced stainless steels were observed at 1000 °C. • GEN IV candidate steels were confronted to classic AISI steels. • ODS AISI 316 has weaker thermal resistance than classic AISI steel. • Ferritic ODS steels and NF 709 has better thermal resistance than AISI steels. - Abstract: Candidate stainless steels for GEN IV reactors were investigated in term of thermal and corrosion stability at high temperatures. New austenitic steel (NF 709), austenitic ODS steel (ODS 316) and two ferritic ODS steels (MA 956 and MA 957) were exposed to around 1000 °C in inert argon atmosphere at pressure of ∼8 MPa. The steels were further studied in a light of vacancy defects presence by positron annihilation spectroscopy and their thermal resistance was confronted to classic AISI steels. The thermal strain supported a creation of oxide layers observed by scanning electron microscopy (SEM).

  2. La política internacional de acceso a los recursos genéticos

    Directory of Open Access Journals (Sweden)

    Martha Isabel Gómez Lee

    2007-11-01

    Full Text Available La biodiversidad es un asunto político. ¿Por qué es importante el acceso e intercambio de los recursos genéticos, y cómo se relaciona con las controversias sobre propiedad intelectual y con las negociaciones ambientales y comerciales en el ámbito internacional? La respuesta a estos interrogantes pone en evidencia un conjunto de reglas, interrelaciones internacionales y nuevos actores que de- finen la política internacional de acceso a los recursos genéticos. El Convenio sobre Diversidad Biológica establece que la diversidad biológica está sometida a la soberanía nacional. ¿El acceso estará determinado por un sistema multilateral o por acuerdos bilaterales?

  3. Recent approaches of fish breeding /Abordagens recentes do melhoramento genético de peixes

    Directory of Open Access Journals (Sweden)

    João Costa Filho

    2012-04-01

    Full Text Available ResumoThis review aims at addressing the main issues related to genetic improvement applied to fish species with zootechnical potential, such as selection, genetic parameters and use of biotechnology as well as address the importance of this area of study for the expansion of aquaculture and its development trend. Fish breeding is one of the most important areas of study for fish production development. Important techniques are applied in order to advance genetic progress in fish breeding (e.g., individual selection, genetic parameters estimation and the use of biotechnology. Traditional breeding in fish farming has been conducted mainly through biometric analysis, which helps in choosing selection criteria and in the assessment of genetic parameters such as heritability and correlations between interesting traits. The use of biotechnology in fish breeding has been successful; it is an important strategy that allows breeding results to be achieved faster, although at a greater cost than traditional breeding. Fish breeding in Brazil is currently in its initial stages; however, it is a field with great growth expectations, and there is still significant research to be conducted in this area.AbstractEsta revisão tem como objetivo a abordagem dos principais aspectos relacionados ao melhoramento genético aplicado em espécies de peixes com potencial zootécnico, tais como seleção, parâmetros genéticos e utilização da biotecnologia, bem como abordar a importância desta área de estudos para a ampliação da aquicultura e sua tendência de desenvolvimento. O melhoramento genético é uma das áreas prioritárias de estudo para o desenvolvimento da produção de peixes. Assim, importantes técnicas são aplicadas visando à obtenção de progresso genético no melhoramento de peixes, como por exemplo, a seleção individual, a estimativa de parâmetros genéticos e o uso da biotecnologia. O melhoramento genético tradicional na piscicultura tem

  4. Medicina Genómica Aspectos éticos, legales y sociales del Genoma Humano

    OpenAIRE

    Rodolfo E. Ávila; María Elena Samar; Leticia Díaz-Beltrán; Esteban, Francisco J.

    2011-01-01

    La Medicina Genómica es el uso de la inf ormación de los genomas y sus deriv ados (ARN, proteínas y met abolitos) que permite guiar la toma de decisiones médicas, es un c omponente clave de la medicina personalizada. La Medicina Genómica permite conocer la cartografía del genoma hum ano y proporciona una valiosa información a tener en cuenta a la hora de detect ar genes implicados en ciert as enfermedades. Esto conlleva a que en la actualidad nos centremos más en la predicción de patologías q...

  5. Manipulação genética e dignidade humana

    OpenAIRE

    Oliveira, Simone Born de

    2001-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Jurídicas Um tema como a manipulação genética e dignidade humana: da bioética ao direito, tem por fim verificar o desenvolvimento da pesquisa científica na área da genética, com suas manipulações e respectivas conseqüências sobre a dignidade humana, a bioética e o direito. Tem que ser possível definir a influência da ética e do direito no desenvolvimento das pesquisas científicas. Para definir essas possibi...

  6. Causas genéticas de deficiência de ferro

    OpenAIRE

    Saad,Sara Teresinha O.

    2010-01-01

    As causas genéticas de deficiência de ferro, real ou funcional, ocorrem por defeitos em muitas proteínas envolvidas na absorção e metabolismo de ferro. Neste capítulo descreveremos sucintamente causas genéticas de carência de ferro para a síntese de hemoglobina, que cursa então com anemia microcítica e hipocrômica. Ressalto que estas são alterações raras, com poucas descrições na literatura. Em alguns casos, o ferro funcional não está disponível para os eritroblastos sintetizarem hemoglobina,...

  7. Estado atual e perspectivas da genética e epidemiologia do alcoolismo

    Directory of Open Access Journals (Sweden)

    Claiton Henrique Dotto Bau

    Full Text Available O alcoolismo é um problema de saúde pública de escala mundial. O abuso e dependência combinados afetam aproximadamente 8% da população brasileira, gerando um grande custo social. O reconhecimento da existência de uma herdabilidade significativa contribuiu para o entendimento do problema como uma doença específica com origem biológica. Os avanços no conhecimento da neurobiologia da dependência permitiram delimitar uma série de genes candidatos para a predisposição. Atualmente, iniciam-se os estudos sobre o papel de polimorfismos genéticos na resposta ao tratamento. A integração de abordagens clínicas, epidemiológicas e de genética molecular pode identificar grupos clínicos mais responsivos a abordagens terapêuticas específicas.

  8. Pentatomiana beckerae gen. nov. and sp. nov., a new Neotropical Pentatomini (Hemiptera, Heteroptera, Pentatomidae

    Directory of Open Access Journals (Sweden)

    Jocélia Grazia

    2004-06-01

    Full Text Available A new genus, Pentatomiana, and a new species, P. beckerae, are described, based on specimens from Rio de Janeiro and Santa Catarina States, Brazil. Brachyptery is present in the males of the new species. Pentatomiana gen. nov. is compared with Lojus McDonald, 1982, which also presents brachyptery in one of the species, but in females. Illustrations of male and female external genitalia are provided.Um novo gênero, Pentatomiana, e uma nova espécie, P. beckerae, são descritos, com base em espécimes do Rio de Janeiro e de Santa Catarina, Brasil. Machos da nova espécie apresentam braquipteria. Pentatomiana gen. nov. é comparado com Lojus McDonald, 1982, o qual também apresenta braquipteria em uma das espécies, mas em fêmeas. São fornecidas ilustrações da genitália externa masculina e feminina.

  9. Dissimilaridade genética entre cultivares de mamoneira por meio de marcadores RAPD

    Directory of Open Access Journals (Sweden)

    Edna Lôbo Machado

    2013-03-01

    Full Text Available O objetivo deste trabalho foi identificar cultivares geneticamente divergentes de mamoneira (Ricinus communis com uso de marcadores RAPD. Um total de 58 iniciadores RAPD foi usado na genotipagem de 15 cultivares. A dissimilaridade genética entre as cultivares foi calculada a partir do índice de Jaccard, tendo-se utilizado o método da média aritmética não ponderada (UPGMA. Foram identificados 552 fragmentos, sendo 311 polimórficos (56,3%. As cultivares foram agrupadas em cinco grupos, evidência de que há divergência genética entre elas. Os marcadores moleculares do tipo RAPD são eficientes no estudo da dissimilaridade em mamoneira.

  10. MOLECULAR PHYLOGENY, ULTRASTRUCTURE, AND TAXONOMIC REVISION OF CHLOROGONIUM (CHLOROPHYTA): EMENDATION OF CHLOROGONIUM AND DESCRIPTION OF GUNGNIR GEN. NOV. AND RUSALKA GEN. NOV.(1).

    Science.gov (United States)

    Nakada, Takashi; Nozaki, Hisayoshi; Pröschold, Thomas

    2008-06-01

    We examined the molecular phylogeny and ultrastructure of Chlorogonium and related species to establish the natural taxonomy at the generic level. Phylogenetic analyses of 18S rRNA and RUBISCO LSU (rbcL) gene sequences revealed two separate clades of Chlorogonium from which Chlorogonium (Cg.) fusiforme Matv. was robustly separated. One clade comprised Cg. neglectum Pascher and Cg. kasakii Nozaki, whereas the other clade included the type species Cg. euchlorum (Ehrenb.) Ehrenb., Cg. elongatum (P. A. Dang.) Francé, and Cg. capillatum Nozaki, M. Watanabe et Aizawa. On the basis of unique ultrastructural characteristics, we described Gungnir Nakada gen. nov. comprising three species: G. neglectum (Pascher) Nakada comb. nov., G. mantoniae (H. Ettl) Nakada comb. nov., and G. kasakii (Nozaki) Nakada comb. nov. We also emended Chlorogonium as a monophyletic genus composed of Cg. euchlorum, Cg. elongatum, and Cg. capillatum. Because Cg. fusiforme was distinguished from the redefined Chlorogonium and Gungnir by the structure of its starch plate, which is associated with pyrenoids, we reclassified this species as Rusalka fusiformis (Matv.) Nakada gen. et comb. nov.

  11. Taxonomic revision of Chlamydomonas subg. Amphichloris (Volvocales, Chlorophyceae), with resurrection of the genus Dangeardinia and descriptions of Ixipapillifera gen. nov. and Rhysamphichloris gen. nov.

    Science.gov (United States)

    Nakada, Takashi; Tomita, Masaru; Wu, Jiunn-Tzong; Nozaki, Hisayoshi

    2016-04-01

    Chlamydomonas (Cd.) is one of the largest but most polyphyletic genera of freshwater unicellular green algae. It consists of 400-600 morphological species and requires taxonomic revision. Toward reclassification, each morphologically defined classical subgenus (or subgroup) should be examined using culture strains. Chlamydomonas subg. Amphichloris is characterized by a central nucleus between two axial pyrenoids, however, the phylogenetic structure of this subgenus has yet to be examined using molecular data. Here, we examined 12 strains including six newly isolated strains, morphologically identified as Chlamydomonas subg. Amphichloris, using 18S rRNA gene phylogeny, light microscopy, and mitochondria fluorescent microscopy. Molecular phylogenetic analyses revealed three independent lineages of the subgenus, separated from the type species of Chlamydomonas, Cd. reinhardtii. These three lineages were further distinguished from each other by light and fluorescent microscopy-in particular by the morphology of the papillae, chloroplast surface, stigmata, and mitochondria-and are here assigned to three genera: Dangeardinia emend., Ixipapillifera gen. nov., and Rhysamphichloris gen. nov. Based on the molecular and morphological data, two to three species were recognized in each genus, including one new species, I. pauromitos. In addition, Cd. deasonii, which was previously assigned to subgroup "Pleiochloris," was included in the genus Ixipapillifera as I. deasonii comb. nov.

  12. Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae.

    Science.gov (United States)

    Bondoso, Joana; Albuquerque, Luciana; Nobre, M Fernanda; Lobo-da-Cunha, Alexandre; da Costa, Milton S; Lage, Olga Maria

    2015-02-01

    Four isolates, belonging to the deep-branching phylum Planctomycetes, were recovered from the biofilm of two marine macroalgae, Ulva sp. and Laminaria sp., from the Northern coast of Portugal. These strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. They had a dimorphic cell cycle with budding reproduction. The organisms were chemoheterotrophic, strictly aerobic and mesophilic. The 16S rRNA gene sequence analysis showed that the strains belong to the family Planctomycetaceae with Rhodopirellula as the closest genus. The isolates form two separate branches (strain LF1(T) forms one branch and the strains UC8(T), UF3 and UF42 form a second branch) clearly separated from Rhodopirellula baltica with 94.2% and 93.8% 16S rRNA gene sequence similarity, respectively. Based on differential characteristics that distinguish the novel genera from R. baltica, such as cell size and shape, ultrastructure, enzymatic activities, substrate utilization pattern, fatty acid composition, phospholipid profiles and phylogeny we propose that the isolates represent two novel genera of the order Planctomycetales, Roseimaritima ulvae gen. nov., sp. nov. (type strain is UC8(T)=DSM 25454(T)=LMG 27778(T)) and Rubripirellula obstinata gen. nov., sp. nov. (type strain is LF1(T)=LMG 27779(T)=CECT 8602(T)).

  13. Two New Freshwater Woloszynskioids Asulcocephalium miricentonis gen. et sp. nov. and Leiocephalium pseudosanguineum gen. et sp. nov. (Suessiaceae, Dinophyceae) Lacking an Apical Furrow Apparatus.

    Science.gov (United States)

    Takahashi, Kazuya; Moestrup, Øjvind; Jordan, Richard W; Iwataki, Mitsunori

    2015-12-01

    Two new woloszynskioid dinoflagellates, Asulcocephalium miricentonis gen. et sp. nov. and Leiocephalium pseudosanguineum gen. et sp. nov., are described from Japanese freshwater ponds on the basis of bright field and fluorescence light microscopy, scanning and transmission electron microscopy, and molecular phylogeny inferred from rDNA sequences. Asulcocephalium miricentonis has a spherical anterior nucleus and chloroplast with a pyrenoid penetrated by the cytoplasm. This species has 9-12 latitudinal series of amphiesmal vesicles (AVs), including an apparently large AV on the right ventral side of the epicone. Leiocephalium pseudosanguineum has a U-shaped nucleus in the epicone and chloroplasts without a pyrenoid. This species has at least 24 latitudinal series of AVs. The characteristic features of both species were brick-like material (type E) in the eyespot and the lack of an apical furrow. These features coincide with those of Polarella glacialis, but the two species differ in cell shape, number and arrangement of AVs, shape of resting cysts, and habitats; i.e., P. glacialis has been reported only from marine cold waters. Molecular phylogeny revealed that A. miricentonis and L. pseudosanguineum were positioned in the Suessiaceae and closely related to Piscinoodinium sp., but their relationship to Polarella and other reported taxa was not supported.

  14. Molecular phylogeny of Urosomoida agilis, and new combinations: Hemiurosomoida longa gen. nov., comb. nov., and Heterourosomoida lanceolata gen. nov., comb. nov. (Ciliophora, Hypotricha).

    Science.gov (United States)

    Singh, Jasbir; Kamra, Komal

    2015-02-01

    For years, systematics of three species, Urosomoida agilis (Engelmann, 1862) Hemberger in Foissner, 1982, Urosomoida longa (Gelei and Szabados, 1950) Foissner et al., 1991 and Oxytricha lanceolata Shibuya, 1930, has remained unresolved due to lack of adequate molecular data. Though, it is known since several years that the three species are not very closely related. In the present paper, 18S rRNA gene sequences for two key species, U. agilis and U. longa, and their morphology and morphometry have been analyzed. Molecular phylogeny inferred from maximum likelihood, neighbour joining and maximum parsimony methods has adequately removed ambiguity over their systematics. In phylogenetic trees, U. agilis clustered consistently with non-stylonychine oxytrichids. Both Urosomoida longa and Oxytricha lanceolata clustered consistently away from U. agilis and O. granulifera, the type species of the genera Urosomoida and Oxytricha, respectively. As a result of the current molecular phylogenetic investigation and based on previously inferred morphological and morphogenetic data it is proposed to remove Urosomoida longa and Oxytricha lanceolata from Urosomoida and incertae sedis in Oxytricha, respectively, and establish two new generic combinations, Hemiurosomoida longa gen. nov., comb. nov. and Heterourosomoida lanceolata gen. nov., comb. nov. for them.

  15. Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

    Directory of Open Access Journals (Sweden)

    Sven eBreider

    2014-08-01

    Full Text Available Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov. and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.

  16. Crescimento de codornas de diferentes grupos genéticos por meio de modelos não lineares

    Directory of Open Access Journals (Sweden)

    L. F. M. Mota

    2015-10-01

    Full Text Available RESUMOObjetivou-se com o presente trabalho ajustar modelos não lineares para descrever o padrão de crescimento de genótipos de codornas de corte e de postura do nascimento aos 35 dias de idade. Foram utilizados dados de 1280 codornas machos e fêmeas, provenientes de sete genótipos de codornas de corte (EV1, EV2, UFV1, UFV2, UFV3, LF1, LF2 e um de postura. Todas as codornas foram pesadas a cada sete dias, do nascimento aos 35 dias de idade. Foram ajustados os modelos Brody, Gompertz, Logístico, Richards e von Bertalanffy aos dados de peso corporal de todos os genótipos. As análises estatísticas foram realizadas utilizando-se o PROC NLIN do SAS (Statistical Analysis System, versão 9.0. Os critérios empregados para a escolha do melhor modelo para descrever a curva de crescimento foram o coeficiente de determinação ajustado (R2, o desvio padrão assintótico (DPA, o desvio médio absoluto dos resíduos (DMA, o índice assintótico (IA, o critério de informação bayesiano (BIC, o critério de Akaike (AIC e o quadrado médio do erro (QME. Todos os modelos não lineares testados convergiram, com exceção do modelo Brody, que não convergiu para nenhum genótipo avaliado. O modelo Richards não convergiu para o genótipo postura. Os parâmetros da curva de crescimento estimados pelos modelos indicaram maior precocidade, em geral, do genótipo postura comparado aos demais genótipos. O modelo Richards apresentou superestimação do ponto de inflexão para todos os genótipos, exceto para o genótipo postura. Os modelos Gompertz, Logístico e von Bertalanffy são recomendados para descrever o crescimento de codornas de corte dos grupos genéticos em estudo. O modelo Brody não convergiu, por isso não é recomendado para descrever o crescimento dos grupos genéticos de codornas em estudo.

  17. Divergência genética entre genótipos de alface por meio de marcadores AFLP Genetics divergence among lettuce genotypes by AFLP markers

    Directory of Open Access Journals (Sweden)

    Cristina Soares de Sousa

    2007-01-01

    Full Text Available Considerando a restrita diversidade de espécies disponíveis para nutrir a carência de vitaminas no Brasil, Kerr e colaboradores, desde 1981, vêm desenvolvendo pesquisas para melhoramento genético de hortaliças ricas em vitamina A. Dentre elas, obtiveram uma cultivar de alface, denominada Uberlândia 10.000 com 10.200 UI de vitamina A em 100 gramas de folha fresca. Este trabalho objetivou comparar o grau de divergência genética entre a cultivar Uberlândia 10.000 e seus parentais para avaliar a eficiência da seleção utilizada, por meio da técnica AFLP. Foram utilizados os seguintes genótipos de alface: Maioba, Salad Bowl-Mimosa, Moreninha-de-Uberlândia, Vitória de Santo Antão, Uberlândia 10.000 lisa 8.ª e 9.ª geração e Uberlândia 10.000 crespa 8.ª e 9.ª geração. A técnica AFLP foi eficiente para identificar genótipos muito próximos e para estudos de progênies em alface. O primer PR15 permitiu a separação da forma lisa e crespa com 1,8% de divergência genética e a oitava da nona geração com apenas 0,71%. Com o estudo da filogenia da cultivar pode-se observar que o programa de melhoramento foi desenvolvido com sucesso, pois a cultivar obtida Uberlândia 10.000 possui alto teor de vitamina A e 92% de similaridade com o parental Vitória de Santo Antão. O primer PR11 conseguiu identificar polimorfismo entre cultivares de alta e baixa resistência à septoriose, sugerindo a possibilidade destas bandas estarem relacionadas à resistência.Considering the restricted diversity of species available to counteract vitamin deficiencies in Brazil, Kerr and coworkers have been engaged since 1981, in developing genetic improved garden vegetables rich in vitamin A. One of these vegetables is the lettuce cultivar Uberlândia 10,000, which contains 10,200 UI of vitamin A per 100 grams of fresh leaves. This study compares the genetic diversity between Uberlândia 10,000 and its parental, evaluating selection efficiency through

  18. Description of Distorhabditis poonchiana n. gen., n. sp. (Nematoda: Rhabditidae) from Jammu and Kashmir, India.

    Science.gov (United States)

    Shah, Ali Asghar; Vaid, Shavish; Hussain, Abid; Ahmad, Rakeeb

    2015-12-01

    Distorhabditis poonchiana n. gen., n. sp. from humus in Jammu and Kashmir, India, is described and illustrated. The new genus is characterized by a small body; slightly setoff labial region; long tubular gymnostom; prominently cuticularized cheilostom; absence of glottoid apparatus; monoprodelphic reproductive system; vulva (V) = 81 to 84; spicules with trifurcated distal ends, simple gubernaculum, peloderan bursa with eight pairs of bursal papillae arranged in 1 + 1 + 1 + 2 + 1 + 2 arrangement.

  19. A new genus, Atlanteuptychia gen. nov., for Euptychia ernestina (Lepidoptera: Nymphalidae: Satyrinae

    Directory of Open Access Journals (Sweden)

    André V. L. Freitas

    2013-12-01

    Full Text Available Atlanteuptychia Freitas, Barbosa & Mielke, gen. nov. is proposed for Euptychia ernestina Weymer, 1911 and illustrated. This taxon lacks the posterior projection of the tegumen, a synapomorphy of Euptychia Hübner, 1818, and does not share morphological synapomorphies with Cyllopsis R. Felder, 1869 and Paramacera Butler, 1868, two Central American genera apparently closely related to Euptychia ernestina, based on molecular data. This evidence supports the proposition of a new genus endemic to the Atlantic Forest, A. ernestina stat. nov.

  20. MicroGen: a MIAME compliant web system for microarray experiment information and workflow management

    Directory of Open Access Journals (Sweden)

    Pinciroli Francesco

    2005-12-01

    Full Text Available Abstract Background Improvements of bio-nano-technologies and biomolecular techniques have led to increasing production of high-throughput experimental data. Spotted cDNA microarray is one of the most diffuse technologies, used in single research laboratories and in biotechnology service facilities. Although they are routinely performed, spotted microarray experiments are complex procedures entailing several experimental steps and actors with different technical skills and roles. During an experiment, involved actors, who can also be located in a distance, need to access and share specific experiment information according to their roles. Furthermore, complete information describing all experimental steps must be orderly collected to allow subsequent correct interpretation of experimental results. Results We developed MicroGen, a web system for managing information and workflow in the production pipeline of spotted microarray experiments. It is constituted of a core multi-database system able to store all data completely characterizing different spotted microarray experiments according to the Minimum Information About Microarray Experiments (MIAME standard, and of an intuitive and user-friendly web interface able to support the collaborative work required among multidisciplinary actors and roles involved in spotted microarray experiment production. MicroGen supports six types of user roles: the researcher who designs and requests the experiment, the spotting operator, the hybridisation operator, the image processing operator, the system administrator, and the generic public user who can access the unrestricted part of the system to get information about MicroGen services. Conclusion MicroGen represents a MIAME compliant information system that enables managing workflow and supporting collaborative work in spotted microarray experiment production.

  1. Narrativa visual: o interplay entre modo e estruturas genéricas

    OpenAIRE

    Silvestre, Carminda; Gonçalves, Madalena

    2010-01-01

    O presente artigo pretende explorar a narrativa visual a partir da variante de registo – o modo – para mostrar que as estruturas genéricas do género são determinadas pelo uso dos recursos semióticos que o produtor de texto escolhe para a concretização da função do texto.

  2. Gen IV Materials Handbook Beta Release for Structural and Functional Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Weiju [ORNL; Luttrell, Claire [ORNL

    2006-09-12

    Development of the Gen IV Materials Handbook is briefly summarized up to date. Current status of the Handbook website construction is described. The developed Handbook components and access control of the beta version are discussed for the present evaluation release. Detailed instructions and examples are given to provide guidance for evaluators to browse the constructed parts and use all the currently developed functionalities of the Handbook in evaluation.

  3. Análisis de la diversidad genética en el Uruguay

    Directory of Open Access Journals (Sweden)

    Álvarez, Inés

    2005-01-01

    Full Text Available El objetivo de nuestro trabajo fue cuantificar el aporte génico diferencial en las diversas regiones del Uruguay. Con vista al objetivo planteado se analizó la distribución de 5 marcadores genéticos clásicos: ABO, RH, HLA-A, HLA-B y HLA-C en 840 uruguayos. La muestra fue estratificada en 4 regiones de acuerdo al lugar de nacimiento de cada individuo: Norte, Centro, Sur y Ciudad de Montevideo. El análisis de la variación de las frecuencias alélicas totales indicó que el nivel de diferenciación interregional es bajo (Gst = 0.0025, lo que señala un bajo nivel de divergencia genética entre las regiones consideradas. Los valores de distancia genética (DA entre las regiones fueron consistentemente bajos; siendo los valores más altos los encontrados entre Montevideo vs. Centro y Centro vs. Sur. Los dendogramas confeccionados sobre la base de las distancias genéticas indican una clara separación de la región Centro de las otras regiones. Los porcentajes de mezcla estimados revelan que el aporte predominante para todas las regiones es el de origen europeo, seguido del amerindio, y por último el africano. Se observó una cierta heterogeneidad en la distribución de los porcentajes de mezcla entre las regiones consideradas, en particular el aporte africano, el que presentó su valor más alto en la región Sur (7.9%, seguido de Montevideo (5.1%. Los resultados obtenidos reflejan un intenso proceso de mestizaje, con posibles variaciones regionales.

  4. Reclassification of Brevibacterium incertum (Breed 1953) as Desemzia incerta gen. nov., comb. nov.

    Science.gov (United States)

    Stackebrandt, E; Schumann, P; Swiderski, J; Weiss, N

    1999-01-01

    Phylogenetic analysis of 16S rDNA indicates that Brevibacterium incertum is not a member of the genus Brevibacterium but related to species of the genus Carnobacterium. Hence, Brevibacterium incertum is not a member of the class Actinobacteria but belongs to the phylogenetically defined broad Bacillus-Lactobacillus cluster. Based upon properties that taxonomically clearly distinguishes Brevibacterium incertum from species of the phylogenetic sister genus Carnobacterium, Brevibacterium incertum is reclassified as Desemzia incerta gen. nov., comb. nov.

  5. Istiochrysis gen. nov., a new chrysidid genus from Oriental China (Hymenoptera, Chrysididae).

    Science.gov (United States)

    Rosa, Paolo; Feng, Jun; Xu, Zai-Fu

    2016-05-18

    Istiochrysis Rosa & Xu, gen. nov. (type species Istiochrysis ziliolii Rosa, Feng & Xu, sp. nov.) in the tribe Chrysidini is described from Oriental part of China (Yunnan). The new genus is related to the genera Caenochrysis Kimsey & Bohart, 1981, Chrysidea Bischoff, 1913, and Trichrysis Lichtenstein, 1876, but can be separated from them by the unique crestate structure of male genitalia, shape of black spots on sternum 2, and facial depressions on scapal basin.

  6. Implementing genetic education in primary care: the Gen-Equip programme.

    Science.gov (United States)

    Paneque, Milena; Cornel, Martina C; Curtisova, Vaclava; Houwink, Elisa; Jackson, Leigh; Kent, Alastair; Lunt, Peter; Macek, Milan; Stefansdottir, Vigdis; Turchetti, Daniela; Skirton, Heather

    2017-03-13

    Genetics and genomics are increasingly relevant to primary healthcare but training is unavailable to many practitioners. Education that can be accessed by practitioners without cost or travel is essential. The Gen-Equip project was formed to provide effective education in genetics for primary healthcare in Europe and so improve patient care. Partners include patient representatives and specialists in genetics and primary care from six countries. Here, we report the progress and challenges involved in creating a European online educational program in genetics.

  7. Fatores genéticos e ambientais envolvidos na carciogênese gástrica

    Directory of Open Access Journals (Sweden)

    César Ana Cristina Gobbo

    2002-01-01

    Full Text Available RACIONAL: O câncer de estômago é o segundo tipo mais comum de neoplasia no mundo. A carcinogênese de estômago é processo de múltiplos passos, podendo manifestar-se em várias etapas como gastrite superficial, gastrite atrófica crônica, metaplasia intestinal, displasia e, finalmente, como um carcinoma. Essas condições costumam ser seqüenciais e ocorrer num período de muitos anos como resultado da exposição a uma variedade de fatores endógenos e exógenos, que causam alterações genéticas. Os recentes avanços da genética molecular têm mostrado que o acúmulo dessas várias anormalidades, incluindo a ativação de oncogenes e a inativação de genes supressores de tumores, resultam no desenvolvimento do câncer. Alterações genéticas descritas em carcinomas gástricos incluem amplificações e mutações dos genes c-ERBB2, K-RAS, c-MET e TP53. O ganho de cromossomos também foi encontrado em várias combinações com perda de outros cromossomos e pode estar associado com a expressão elevada de oncogenes, que contribuem com a progressão tumoral. CONCLUSÃO: Essas mudanças genéticas em carcinomas evidenciam o processo de múltiplas etapas da carcinogênese gástrica, por meio do acúmulo de uma série de alterações.

  8. Resolution of single and double Holliday junction recombination intermediates by GEN1.

    Science.gov (United States)

    Shah Punatar, Rajvee; Martin, Maria Jose; Wyatt, Haley D M; Chan, Ying Wai; West, Stephen C

    2017-01-17

    Genetic recombination provides an important mechanism for the repair of DNA double-strand breaks. Homologous pairing and strand exchange lead to the formation of DNA intermediates, in which sister chromatids or homologous chromosomes are covalently linked by four-way Holliday junctions (HJs). Depending on the type of recombination reaction that takes place, intermediates may have single or double HJs, and their resolution is essential for proper chromosome segregation. In mitotic cells, double HJs are primarily dissolved by the BLM helicase-TopoisomeraseIIIα-RMI1-RMI2 (BTR) complex, whereas single HJs (and double HJs that have escaped the attention of BTR) are resolved by structure-selective endonucleases known as HJ resolvases. These enzymes are ubiquitous in nature, because they are present in bacteriophage, bacteria, archaea, and simple and complex eukaryotes. The human HJ resolvase GEN1 is a member of the XPG/Rad2 family of 5'-flap endonucleases. Biochemical studies of GEN1 revealed that it cleaves synthetic DNA substrates containing a single HJ by a mechanism similar to that shown by the prototypic HJ resolvase, Escherichia coli RuvC protein, but it is unclear whether these substrates fully recapitulate the properties of recombination intermediates that arise within a physiological context. Here, we show that GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates. Moreover, we find that GEN1 exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. These results contrast with those obtained with RuvC, which exhibits a strict requirement for the consensus sequence 5'-(A)/TTT(G)/C-3'.

  9. LMC y detección del gen de fusión BCR/ABL

    Directory of Open Access Journals (Sweden)

    C. G. Artigas Allaire

    2006-04-01

    Full Text Available La anormalidad citogenética más común en la leucemia mieloide crónica (LMC es el cromosoma Philadelphia producida por la translocación t(9:22, cuya expresión molecular es el gen de fusión BCR-ABL, que codifica una proteína con actividad tirosinquinasa.

  10. Dual-track CCS stakeholder engagement: Lessons learned from FutureGen in Illinois

    Science.gov (United States)

    Hund, G.; Greenberg, S.E.

    2011-01-01

    FutureGen, as originally planned, was to be the world's first coal-fueled, near-zero emissions power plant with fully integrated, 90% carbon capture and storage (CCS). From conception through siting and design, it enjoyed strong support from multiple stakeholder groups, which benefited the overall project. Understanding the stakeholder engagement process for this project provides valuable insights into the design of stakeholder programs for future CCS projects. FutureGen is one of few projects worldwide that used open competition for siting both the power plant and storage reservoir. Most site proposals were coordinated by State governments. It was unique in this and other respects relative to the site selection method used on other DOE-supported projects. At the time of site selection, FutureGen was the largest proposed facility designed to combine an integrated gasification combined cycle (IGCC) coal-fueled power plant with a CCS system. Stakeholder engagement by states and the industry consortium responsible for siting, designing, building, and operating the facility took place simultaneously and on parallel tracks. On one track were states spearheading state-wide site assessments to identify candidate sites that they wanted to propose for consideration. On the other track was a public-private partnership between an industry consortium of thirteen coal companies and electric utilities that comprised the FutureGen Alliance (Alliance) and the U.S. Department of Energy (DOE). The partnership was based on a cooperative agreement signed by both parties, which assigned the lead for siting to the Alliance. This paper describes the stakeholder engagement strategies used on both of these tracks and provides examples from the engagement process using the Illinois semi-finalist sites. ?? 2011 Published by Elsevier Ltd.

  11. Kontrolle der Expression des UNUSUAL FLORAL ORGANS (UFO) Gens in Arabidopsis thaliana

    OpenAIRE

    Hobe, Martin

    2004-01-01

    Die vorliegende Arbeit befaßt sich mit der Kontrolle des Expressionsmusters des UNUSUAL FLORAL ORGANS (UFO) Gens von Arabidopsis thaliana. UFO wird im Sproß- und Blütenmeristemen aller Entwicklungsstadien der Pflanze exprimiert. In Blütenmeristemen agiert UFO als Kofaktor von LEAFY (LFY) bei der Aktivierung der Organidentitätsgene des zweiten und dritten Wirtels. UFO stellt also einen generellen Faktor der Musterbildung in Meristemen dar. Um regulatorische Gene, die die Expression von UFO bee...

  12. Donde la genética no llega, la ciencia alcanza

    OpenAIRE

    FAMMA

    2008-01-01

    Científicamente nombrada como “osteogénesis imperfecta” y popularmente conocida como “huesos de cristal” esta patología consiste en una malformación de los huesos desencadenada por la mutación de un gen encargado de producir el colágeno una proteína esencial responsable de dar rigidez a los huesos.

  13. Donde la genética no llega, la ciencia alcanza

    OpenAIRE

    2008-01-01

    Científicamente nombrada como “osteogénesis imperfecta” y popularmente conocida como “huesos de cristal” esta patología consiste en una malformación de los huesos desencadenada por la mutación de un gen encargado de producir el colágeno una proteína esencial responsable de dar rigidez a los huesos.

  14. Diversidad genética de las variedades de arroz FLAR liberadas entre 2003-2014.

    Directory of Open Access Journals (Sweden)

    Luis Eduardo Berrio-Orozco

    2016-06-01

    Full Text Available El objetivo de este trabajo fue determinar la base genética, el coeficiente de parentesco y la diversidad genética de las variedades de arroz liberadas entre el 2003 - 2014 en trece países miembros del Fondo Latinoamericano para Arroz de Riego (FLAR. Para ello, se analizaron las genealogías de 51 variedades, en el Centro Internacional de Agricultura Tropical (CIAT, Palmira, Colombia, durante los años 2014 y 2015. La variabilidad genética de las variedades estuvo representada por 120 ancestrales (2,4 ancestral/variedad; 33 de estos contribuyeron con el 83,9% de sus genes, de los cuales tres (ancestrales que originaron IR8 aportaron el 35,6% de sus genes. También se mostró que el coeficiente de parentesco (rxy entre las diferentes variedades comerciales varió de 0,03 (muy poco relacionadas, hasta 0,99 (altamente relacionadas. El promedio de todas las 51 variedades fue de (rxy 0,19. A nivel de variedades por país, se encontró que el promedio fue variable, el mínimo lo obtuvo Ecuador con 0,13, y el máximo fue de 0,31 para las variedades de Venezuela. El análisis de agrupamiento separó a los genotipos en catorce grupos distintos, donde existen materiales bastante relacionados y otros muy poco relacionados. Estos resultados muestran que se ha obtenido una ampliación de la base genética. 

  15. Solving the TTC 2011 Reengineering Case with GrGen.NET

    CERN Document Server

    Jakumeit, Edgar; 10.4204/EPTCS.74.16

    2011-01-01

    The challenge of the Reengineering Case is to extract a state machine model out of the abstract syntax graph of a Java program. The extracted state machine offers a reduced view on the full program graph and thus helps to understand the program regarding the question of interest. We tackle this task employing the general purpose graph rewrite system GrGen.NET (www.grgen.net).

  16. Solving the TTC 2011 Reengineering Case with GrGen.NET

    Directory of Open Access Journals (Sweden)

    Edgar Jakumeit

    2011-11-01

    Full Text Available The challenge of the Reengineering Case is to extract a state machine model out of the abstract syntax graph of a Java program. The extracted state machine offers a reduced view on the full program graph and thus helps to understand the program regarding the question of interest. We tackle this task employing the general purpose graph rewrite system GrGen.NET (www.grgen.net.

  17. Estudios de transformación genética en arveja voluble cultivar Santa Isabel

    Directory of Open Access Journals (Sweden)

    María Isabel Peñaranda

    2013-12-01

    Full Text Available Título corto: Estudios de transformación genética en arveja.Resumen: Se probaron diferentes alternativas de transformación genética en arveja cultivar “Santa Isabel” con el fin de estudiar los factores que afectan el proceso. Se emplearon los métodos de infiltración mediante vacío, infección directa de explantes, transformación de polen, y microinyección de ovarios. La prueba histoquímica de expresión gus fue escogida como método de análisis en la determinación de transformantes positivos. Con las metodologías empleadas se detectaron puntos azules en el tejido vegetal, lo cual indica la expresión transitoria del transgen en los explantes utilizados. Los resultados obtenidos sugieren que la transformación genética en arveja cultivada en Colombia puede ser utilizada para la introducción de genes de interés como apoyo a los procesos de mejoramiento genético.Palabras clave: genotipos, expresión gus, fitomejoramiento.Abstract: Different genetic transformation alternatives were tested in “Santa Isabel” cultivar pea, in order to study the factors that affect the process. The methods used were infiltration with vacuum, direct infection of the explants, pollen transformation and ovary microinjection. The hystochemical test of  gus expression was chosen as analysis method in the determination of positive transformants. Blue spots in the plant tissue were detected, with the used methodologies, which indicates transient expression of the transgene in the explants utilized. The obtained results suggest that the genetic transformation in pea genotypes planted in Colombia can be used for the introduction of genes of interest as support to genetic improvement.Key words: genotypes, gus expressions, plant breeding.

  18. Saber o no saber… Derecho e información genética

    Directory of Open Access Journals (Sweden)

    José Ignacio Solar Cayón

    2013-12-01

    Full Text Available El extraordinario desarrollo de las técnicas genéticas, con su formidable capacidad de afectación a la autonomía personal y de invasión de los derechos individuales, está teniendo un impacto profundo en el pensamiento jurídico, obligándonos a revisar algunos de los presupuestos en que se funda nuestra concepción de los derechos fundamentales. Así, el reconocimiento del derecho del individuo a no conocer sus datos genéticos parece desafiar nociones esenciales como las de autonomía y racionalidad del sujeto de derechos, vinculadas en el proyecto ilustrado de emancipación del individuo a la idea de pleno acceso al conocimiento. Sin embargo, la propia idea de “ignorancia” no resulta ajena al discurso de fundamentación de los derechos fundamentales, como prueba el papel esencial que el “velo de la ignorancia” desempeña en la revisión de la tradición liberal efectuada por John Rawls. A partir de la teoría de éste y de John Stuart Mill se indaga en los fundamentos filosóficos del derecho a no saber los datos genéticos y en sus límites, ante la existencia de posibles derechos de terceros a acceder a esa información. Asimismo, se pone de manifiesto el papel que en este nuevo contexto juega el Derecho como instancia administradora del conocimiento y de la ignorancia, ante la amenaza de un determinismo genético que parece poner en cuestión en última instancia la idea misma de libertad individual.

  19. Biomek®-3000 and GenPlex SNP Genotyping in Forensic Genetics

    DEFF Research Database (Denmark)

    Stangegaard, Michael; Tomas, Carmen; Hansen, Anders J.;

    2008-01-01

    Single nucleotide polymorphism genotyping provides a supplement for conventional short tandem repeats-based kits currently used for human identification. GenPlex (Applied Biosystems (AB), Foster City, CA) is an SNP-genotyping kit based on a multiplex of 48 informative, autosomal SNPs from...... but one sample. The results demonstrate that the Biomek-3000 can perform a series of complex reactions leading to highly consistent forensic genetic SNP-typing results Udgivelsesdato: 2008/10...

  20. Studies on Parataxospermum taiyuanensis gen. et sp. nov. from coal balls

    Energy Technology Data Exchange (ETDEWEB)

    Li, Z.M. (Academia Sinica, Beijing (China). Institute of Botany)

    1993-05-03

    This paper reports on a new genus and a new species of the Order Cardiocarpales, Parataxospermum taiyuanensis gen. et sp. nov., which is described from coal balls of the Upper Carboniferous (or lower Permian) in the Shanxi Province, China. The taxon is described and its taxonomic position and affinities are discussed in detail. In addition, a three-dimensional reconstruction technique using computer graphics is utilized to reconstruct external images of the seeds.