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Sample records for frijol colorado phaseolus

  1. Estudio de la fitohemaglutinina proveniente del frijol colorado (Phaseolus Vulgaris Study of phytohemagglutinin from red bean (Phaseolus vulgaris

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    Patricia Hernández Díaz

    1999-12-01

    Full Text Available Se obtuvieron preparaciones crudas de fitohemaglutinina (PHA a partir de frijol colorado (Phaseolus vulgaris por 2 métodos: extracción acuosa y extracción ácida. Por el método ácido se obtuvieron mejores resultados en cuanto a concentración de proteínas y pureza de la misma. Se empleó la cromatografía en hidroxiapatita para separar las isoformas que forman la PHA y se obtuvieron 5 fraccciones proteicas que se evaluaron funcionalmente junto con las preparaciones crudas, a través de sus propiedades eritroaglutinantes y leucoaglutinantes. A medida que se aumentó la fuerza iónica del medio se observó que las fracciones obtenidas presentaban una disminución relativa de la actividad leucoaglutinante, así como un incremento relativo de la actividad eritroaglutinante. Las preparaciones crudas y las fracciones se evaluaron electroforéticamente y se obtuvieron bandas muy similares a la de la PHA patrón utilizadaRaw preparations of phytohemagglutinin (PHA were obtained from red bean (Phaseolus vulgaris by 2 methods: aqueous extraction and acid extraction. Better results were obtained with the acid methods as regards the protein concentration and its purity. Hydroxyapatite chromatography was used to separate the isoforms forming the phytohemagglutinin. 5 protein fractions were obtained and functionally evaluated together with the raw preparations through their erythroagglutinating and leukoagglutinating properties. As the ionic force of the medium increased it was observed that the fractions obtained presented a relative decrease of the leukoagglutinating activity as well as a relative rise of the crythroagglutinating activity. The raw preparations and the fractions were electrophoretically evaluated and bands very similar to that of the PHA used as a pattern were obtained

  2. Genotipos de frijol (Phaseolus Vulgaris l. resistentes a Xanthomonas campestris pv. phaseoli de Mexico

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    Rosa Navarrete

    2000-01-01

    Full Text Available Genotipos de frijol (Phaseolus vulgaris L. resistentes a Xanthomonas campestris pv. phaseoli de México. Durante 1995 se evaluó la reacción de genotipos de frijol de diversos origenes a Xcp, bajo condiciones de invernadero en el Campo Experimental del Valle de México, del INIFAP. Se realizaron tres experimentos con a120, b44 y csiete genotipos de frijol. Las plantas se inocularon por corte con navajas en la etapa V3, a y b con una mezcla de nueve cepas de Xcp y el c, con cada una de siete cepas con diferente grado de patogenicidad. La severidad se evaluó 20 días después de la inoculación, por comparación con una escala visual de nueve grados. Los datos se analizaron bajo un diseño completamente al azar. En a, los genotipos que mostraron reacción de resistencia a Xcp fueron: A 36, A 475, G 5686, G 11867, Harowood, SEA 14, XAN 266, MCD 4012 y REN 27. En b los genotipos resistentes fueron: Sequía Durango, Taylor y XAN 30. En los experimentos anteriores la severidad de la enfermedad mostró una distribución normal, con el máximo número de genotipos en el grado de severidad cinco en a y seis en b. Los resultados obtenidos indican que el uso de mezclas de cepas de bacterias con diferente patogenicidad es eficiente para identificar genotipos de frijol resistentes a Xcp. Los genotipos resistentes identificados en el último experimento, mostraron respuesta diferencial e interacciones genotipo por cepa. REN 27 y SEA 14 mostraron resistencia a las cepas utilizadas

  3. Papel del frijol negro Phaseolus vulgaris en el estado nutricional de la población guatemalteca

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    Serrano, José; Goñi, Isabel

    2004-01-01

    RESUMEN. En Guatemala existe un fenómeno de superposición epidemiológica, en el que coexisten problemas de salud propios de países desarrollados con otros característicos de poblaciones en vías de desarrollo. Se observan deficiencias marcadas en algunos macronutrientes tales como hierro y vitamina A. en simultaneidad con enfermedades crónicas como diabetes tipo II o enfermedades cardiovasculares. Se conoce muy bien la importancia del frijol negro (Phaseolus vulgaris) en la dieta habitual de G...

  4. Fraccionamiento de nitrógeno en frijol ( phaseolus vulgaris l. en el valle de san juan

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    Juan Cedano

    2000-01-01

    Full Text Available Fraccionamiento de nitrógeno en frijol (Phaseolus vulgaris L. en el valle de San Juan. Se realizó un estudio para determinar el efecto del fraccionamiento de la fertilización nitrogenada y el momento adecuado para la aplicación de nitrógeno en el cultivo de frijol (Phaseolus vulgaris L. en cinco localidades del Valle de San Juan, R. D. Los experimentos fueron establecidos del 5 al 14 de noviembre (1997 , se utilizó un diseño de bloques completamente al azar y nueve tratamientos en cada localidad, encontrándose que en los terrenos con altos niveles de nitrógeno no hubo respuesta a la aplicación de nitrógeno ni al fraccionamiento de este nutrimento; mientras que en los suelos con deficiencia en nitrógeno si hubo respuesta a la fertilización nitrogenada encontrándose diferencia estadística significativa a la aplicación y al momento de aplicación del fertilizante. Entre las localidades hubo diferencia estadística significativa (P>0.05, mientras que no se encontró interacción entre los tratamientos y las localidades

  5. Estrategia para el manejo de suelos ácidos en frijol (phaseolus vulgaris l. en el estado de Chiapas, Mexico

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    Bernardo Villar S\\u00E1nchez

    2000-01-01

    Full Text Available Estrategia para el manejo de suelos ácidos en frijol (Phaseolus vulgaris L. en el estado de Chiapas, México. Por su contribución en la dieta alimenticia y la generación de empleos, y por existir una ancestral cultura productiva y una superficie de siembra de más de 100 mil hectáreas, el cultivo de frijol es de fundamental importancia en el estado de Chiapas. Sin embargo, se ha determinado una brecha tecnológica de más de 700 kg/ha entre el potencial del cultivo y los rendimientos actuales. Esta es ocasionada por numerosos factores limitantes entre los que destaca la presencia de suelos de baja fertilidad y ácidos. La estrategia adoptada para el manejo de este problema incluye: 1.Aplicación de cal y fósforo; 2. Mejoramiento genético para resistencia; 3.Manejo de los ciclos de materia orgánica y nutrientes del suelo; y 4.Combinación de las tres alternativas. En este reporte se dan los avances obtenidos con relación a la primera alternativa considerada como el paso inicial para el logro de un manejo integral de suelos en Chiapas. Durante 1997 se estudiaron cinco dosis de cal y tres de fósforo en condiciones de invernadero y campo para suelos de diferentes localidades. Se utilizó un experimento factorial completo con cuatro repeticiones, los experimentos fueron evaluados en términos de la producción de grano y al efecto de la cal sobre las propiedades químicas del suelo. Hubo respuesta del frijol en rendimiento a la aplicación de cal y fósforo

  6. Efecto inhibitorio de los taninos del frijol carioca (Phaseolus vulgaris L.) sobre la digestibilidad de la faseolina por dos sistemas multienzimáticos

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    Del Pino,Victoria H.; Lajolo,Franco M.

    2003-01-01

    Cantidades variables de dos sistemas multienzimáticos de tripsina-quimotripsina-peptidasa y pepsina-pancreatina, fueron utilizados para evaluar el efecto de los taninos provenientes de frijol Carioca (Phaseolus vulgaris L.) sobre la digestibilidad de la faseolina, en las formas nativa y denaturalizada. Esta evaluación hecha por los métodos de caida de pH, de hidrólisis en medio tamponado con posterior medición del grado de hidrólisis con ninhidrina y por la técnica electroforética, demostró e...

  7. Mejoramiento de la producción del frijol (Phaseolus vulgaris, L con el uso de alternativas de fertilización

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    Rolando Ramírez Olivera

    2010-01-01

    Full Text Available Argumenta la evaluación de algunas alternativas de nutrición en el frijol común (Phaseolus vulgaris, L., variedad Velasco Largo, que se desarrolló en áreas de la subestación de Granos de la Unidad de Extensión, Investigación y Capacitación Agropecuaria de Holguín (UEICA-H, ubicada en Velasco, municipio de Gibara, provincia de Holguín, en el periodo comprendido entre diciembre de 2007 y marzo de 2008. Se aplicaron seis (6 variantes de fertilización, que comprendió el uso de fertilizantes químicos a dosis menores que las recomendadas, la utilización de abonos orgánicos: humus de lombriz tanto sólido como líquido y el biofertilizante Ecomic. Se tomaron mediciones de altura de las plantas, nodulación y rendimiento y sus componentes, así como, se realizó un análisis económico en base a los rendimientos obtenidos. Los resultados mostraron que la utilización de diferentes alternativas de fertilización provocó un efecto directo sobre el crecimiento de las plantas de frijol, sobre la nodulación natural y el rendimiento y sus componentes, los mismos sugieren la utilización de combinaciones de fertilizantes químicos, orgánicos y biofertilizantes para la obtención de altos rendimientos de forma sostenible en el cultivo del frijol.

  8. Efecto de un promotor del crecimiento activado molecularmente sobre la germinación y la producción de frijol (Phaseolus vulgaris L..

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    Kolima Peña Calzada

    2015-09-01

    Full Text Available El objetivo de la investigación fue evaluar el efecto de un promotor del crecimiento activado molecularmente sobre la germinación y producción del cultivo de frijol (Phaseolus vulgaris L.. Se hicieron dos experimentos, uno in vitro con un diseño completamente aleatorizado y otro en campo con dos variantes y 8 réplicas (diseño de bloques al azar. El tratamiento en ambos ensayos fue inmersión de las semillas en una solución del promotor al 0,02% por 3 horas y otro grupo testigo con inmersión por igual tiempo en agua natural. Los resultados mostraron que la germinación y la altura de las plantas fueron mejores con la inmersión en el producto en ambos experimentos. En el ensayo de campo los vainas por planta no hubo diferencia entre los tratamientos. En los granos por vaina y granos por planta el grupo tratado fue superior al control en 16,70 y 15,22% respectivamente. La masa de 100 granos no mostró diferencia entre ambos tratamientos. El rendimiento agrícola fue superior en los tratados(1,22t.ha-1 vs. 1,02. Con ambos experimentos se determinó que el promotor del crecimiento influyó positivamente en la germinación, desarrollo y producción del cultivo del frijol.

  9. Efectos de la aplicación simultanea de Fitomas E y Biobras 16 en el cultivo del frijol (Phaseolus vulgaris L..

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    Kolima Peña Calzada

    2015-09-01

    Full Text Available El objetivo fue evaluar el efecto de la aplicación simultánea de Fitomas E y Biobras 16 en el cultivo del frijol (Phaseolus vulgaris L.. Se utilizó un diseño de bloques al azar con dos tratamientos y ocho réplicas. Los tratamientos consistieron en la aplicación combinada de Fitomas E (2,0 l.ha-1 y Biobras 16 (0,12 l.ha-1 y una variante control. Las variables evaluadas fueron: altura de las plantas a los 25 y 40 días post siembra, vaina por planta, granos por vainas, granos por plantas, masa de 100 granos y rendimiento agrícola. Se observó en la altura de las plantas diferencias estadísticas significativas entre ambos tratamientos, superando la combinación de Fitomas E y Biobras 16 al tratamiento control, en un 19,43 % y un 17,73 % respectivamente. La variable vainas por planta también mostró diferencias significativas favorables al tratamiento con los bioestimulantes, no así el número de granos por vainas y la masa de 100 granos. El mayor rendimiento agrícola se obtuvo con la variante donde se aplicó Fitomas E y Biobras 16, el cual difirió significativamente del control. Se concluye que el comportamiento agroproductivo del cultivo del frijol (Phaseolus vulgaris L. se favoreció con el tratamiento evaluado.

  10. EFECTIVIDAD DE UN BIOFERTILIZANTE FOLIAR SOBRE EL CULTIVO DE FRIJOL COMÚN (PHASEOLUS VULGARIS, BLUEFIELDS, R.A.A.S.

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    Yader Mejía Bermúdez

    2012-01-01

    Full Text Available Este trabajo tuvo como propósito evaluar la efectividad de las diferentes dosis de un biofertilizante foliar sobre el rendimiento del cultivo de frijol común (Phaseolus vulgaris en época de postrera, en la comunidad El Cañal, Kukra River, Bluefields, en el ciclo productivo 2008-2009. Se utilizó un diseño completo al azar, cuatro tratamientos con dos repeticiones, cada repetición constó de 63 plantas de frijol (n=126. Los tratamientos fueron: T1 (testigo; T2: 0.25 litros de biofertilizante; T3: 0.37 lt. y T4: 0.5 lt. En todos los tratamientos, incluyendo al testigo, se hizo deshierbe y en todos, excepto el testigo, se aplicó el biofertilizante disuelto en 10 litros de agua. Se realizaron tres mediciones (21, 36 y 51 días después de la siembra.Los resultados indican que no existen diferencias significativas entre los tratamientos en la altura de las plantas, pero si en el porcentaje de afectación por herbívoros, así como en el número de vainas por planta y de granos por vaina. El tratamiento T4 registró el menor ataque por herbívoros, la mayor producción en función del número de vainas por planta, de granos por vaina y peso total de los granos de frijol, en cambio el testigo fue el que registró el menor rendimiento con respecto a todas estas variables. El uso del biofertilizante foliar aumentó el rendimiento del cultivo de frijol en todas las dosis.SummaryThe aim of this work was to evaluate the effectiveness of different doses of a foliar biofertilizer on crop production of common bean (Phaseolus vulgaris throughout season time in the community of El Cañal, Kukra River, Bluefields, during the production cycle 2008-2009. A random design was used, four treatments with two replications, each replication consisted of 63 bean plants (n=126. The treatments were: T1 (baton, T2: 0.25 liters of biofertilizer, T3: 0.37 lt. and T4: 0.5 lt.  In all the treatments, including the baton, weeding was done, and in all except the baton

  11. EFECTO DEL ABONO DE SUSTRATO GASTADO DE CHAMPIÑÓN EN EL RENDIMIENTO DE FRIJOL Phaseolus vulgaris L.

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    Helio Adán García Mendívil

    2014-01-01

    Full Text Available La manipulación de los desechos orgánicos y sus compostas representan una fuente de nutrientes imprescindible para la agricultura sostenible, ya que provee de fertilizantes naturales y reduce la contaminación ambiental, bajando con ello el costo de producción. El objetivo de este estudio fue evaluar el efecto de la aplicación de composta a base de sustrato gastado de champiñón, enriquecida con silicio, al suelo, en cultivo de frijol, a través de mediciones fisiológicas y rendimiento en frijol. El experimento se estableció en campo, sembrándose frijol var. Pinto Saltillo en Huatabampo, Sonora, en un suelo de tipo aluvión ligero. El diseño experimental utilizado fue de bloques completos al azar, compuesto de 4 tratamientos con cuatro repeticiones: 1.- testigo (sin aplicación, 2-2.5, 3 5 y 4-7.5 Mg ha 1 de composta. Las variables evaluadas fueron: clorofila total, tasa fotosintética (µg CO2 S-1 cm-2, número de vainas por planta y rendimiento. Con los datos se realizó un análisis de varianza y las medias se compararon con la prueba de Tukey (P ≤ 0.05. Se observaron respuestas significativas en la mayoría las variables evaluadas, en clorofila total hubo aumentos de hasta 26.2% en el tratamiento dos, para fotosíntesis de 26.1% en el tratamiento cuatro, en el número de vainas 8.7% en el tratamiento tres, siendo este el de mayor rendimiento (P ≤ 0.05 con 2.91 Mg ha-1, seguido del tratamiento dos con 2.89 Mg ha-1, el tratamiento cuatro con 2.85 Mg ha-1, y por último el testigo con 1.4 Mg ha 1. Los resultados sugieren que el empleo de composta es una alternativa de fertilización viable para la producción de frijol.

  12. Efecto inhibitorio de los taninos del frijol carioca (Phaseolus vulgaris L.) sobre la digestibilidad de la faseolina por dos sistemas multienzimáticos Inhibitory effect of carioca bean tannins on phaseolin digestibility assessed by two multi-enzymatic systems

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    Victoria H. Del Pino; Franco M. Lajolo

    2003-01-01

    Cantidades variables de dos sistemas multienzimáticos de tripsina-quimotripsina-peptidasa y pepsina-pancreatina, fueron utilizados para evaluar el efecto de los taninos provenientes de frijol Carioca (Phaseolus vulgaris L.) sobre la digestibilidad de la faseolina, en las formas nativa y denaturalizada. Esta evaluación hecha por los métodos de caida de pH, de hidrólisis en medio tamponado con posterior medición del grado de hidrólisis con ninhidrina y por la técnica electroforética, demostró e...

  13. Efecto inhibitorio de los taninos del frijol carioca (Phaseolus vulgaris L. sobre la digestibilidad de la faseolina por dos sistemas multienzimáticos

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    Del Pino Victoria H.

    2003-01-01

    Full Text Available Cantidades variables de dos sistemas multienzimáticos de tripsina-quimotripsina-peptidasa y pepsina-pancreatina, fueron utilizados para evaluar el efecto de los taninos provenientes de frijol Carioca (Phaseolus vulgaris L. sobre la digestibilidad de la faseolina, en las formas nativa y denaturalizada. Esta evaluación hecha por los métodos de caida de pH, de hidrólisis en medio tamponado con posterior medición del grado de hidrólisis con ninhidrina y por la técnica electroforética, demostró el efecto adverso de los taninos condensados sobre la digestibilidad de la faseolina, después de producirse una significativa inhibición en el grado de hidrólisis de esa proteína por los dos sistemas multienzimáticos. Se comprobó la dificultad de hidrólisis de la faseolina cuando esta unida al tanino en el punto de saturación (proporción 5/20 tanino/proteína p/p y mayores, después de detectarse en los perfiles electroforéticos, péptidos de 45.7 y 24KDa, resistentes a la hidrólisis hasta por prolongados períodos de incubación. Esto se debió a la existencia de un complejo de naturaleza refractária no digeríble, incluso en condiciones excesivas de enzima. Los resultados sugieren la unión simultánea del tanino con mas de un ligante a través de sus múltiples grupos hidróxilos libres, formando un complejo proteína-tanino-enzima (PTE* que correspondería a la fracción no digeríble de la faseolina.

  14. Características morfo-fisiológicas de frijol común (Phaseolus vulgaris L. relacionadas con la adaptación a sequía

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    José A Polanía

    2012-07-01

    Full Text Available El desarrollo de variedades adaptadas es una de las estrategias que contribuye a garantizar la seguridad alimentaria en zonas productoras de frijol con estrés por sequía. En los invernaderos de cristal del Centro Internacional de Agricultura Tropical (CIAT, Palmira, Colombia, se evaluaron las características morfológicas y fisiológicas de raíces de 21 líneas de frijol (Phaseolus vulgaris L. en condiciones de estrés por sequía e irrigación, utilizando un sistema de tubos plásticos desarrollado por el CIAT. Las características evaluadas fueron profundización visual, longitud total de raíces y distribución de raíces a través del perfil del suelo. En campo, utilizando los mismos genotipos de frijol, se evaluaron características relacionadas con la eficiencia de uso de agua mediante la técnica de discriminación de isótopo de carbono y producción de grano. Los resultados en invernadero mostraron que la profundización visual y longitud de raíces entre 60 y 75 cm tienen una correlación significativa (r = 0.56 y 0.50 respectivamente, P < 0.001 con la biomasa aérea de la planta en condiciones de estrés por sequía. En campo, la discriminación del isótopo de carbono en grano se correlacionó (r = 0.32, P < 0.01 con la producción de grano en condiciones igualmente de estrés por sequía. Las líneas de frijol NCB 226, SER 16, SEN 56 y SEA 15 presentaron una mayor longitud de raíces bajo estrés por sequía (28, 24, 29 y 27 m/planta respectivamente en comparación con las demás líneas evaluadas, lo que les permite mayor transpiración y movilización de fotoasimilados, que favorecen una alta producción de granos. Se estima que la profundización visual, la longitud de raíces entre 60 y 75 cm y la discriminación de isótopo de carbono en grano, son características valiosas como criterios de selección en mejoramiento por tolerancia a estrés por sequía en frijol común.

  15. Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L.) en Costa Rica

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    Carlos M. Araya; Rodolfo Araya

    2000-01-01

    En esta investigación se evaluaron diversos genotipos de frijol en la búsqueda de fuentes de resistencia a la antracnosis (Colletotrichum lindemuthianum) y a la mancha angular (Phaeoisariopsis griseola). Se evaluó germoplasma del programa nacional de hibridaciones y de tres viveros internacionales distribuidos por el CIAT: VIFURE, VIPADOGEN y CORE COLLECTION. Los ensayos se establecieron en Puriscal (1017 msnm), Alajuela (814 msnm) y Fraijanes (1650 msnm), durante los ...

  16. Growth dynamics of two bean varieties (Phaseolus vulgaris L.. l. Growth analysis by fixed periods Dinámica de crecimiento de dos variedades de frijol (phaseolus vulgaris L. l. análisis de crecimiento por periodos fijos

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    Maldonado Gustavo

    1993-12-01

    Full Text Available Inthe vegetative phase of growth in bean (Phaseolus vulgaris L., the dry matter gain depends on the physiological behavior and growth habit of the bean plant. The Growth process in relation to growth type were evaluated in the present study. The purpose of the study was to establish growth type and growth index relations in ICA-Cerinza (growth type 1 and
    ICA -Tundama (growth type 11 wich are both bush bean varieties. The plants were sown in 40.5 m2 plots, in rows spaced 0.5 m. and 0.12 m. between plants in a randomized completely block design with 4 replications; samples of 3 plants per plotwere taken 7 days each starting 15 to 78 days after emergence. Total dryweight (TOW Stem dryweight (SOW, leaf dry weight (LOW and total leaf area (TLA were
    determined. Relative growth rate (RGR, net assimilation rate (NAR, leaf area ratio (LAR, leaf weight ratio (LWR, and specific leaf area (SLA, were determined by the classical aproach. Significant differences in TDW, SDW, LDW, and TLA between varieties were detected in the 15 and 78 days
    evaluations. Similar results were obtained for RGR, NAR, LAR, LWR, and SLA. The mean RGR was 0.0455 g. g-1 .day-1 in Cerinza and 0.0437 g.g-1.day -1 in Tundama but these were no statistically different. NAR and RGR had similar trends and were positive and significantly correlated. LAR decreced
    linearly in Cerinza, but it was cuadratic in Tundama with the highest values in the initial and the last evaluations. The LWR show that Tundama variety translocated more dry matter to assimilatory tissue formation. SLA was similar for the two varieties, but it was higher in the indeterminate (type 11, showing that Tundama had moretinny leaves. Growth analysis
    utilizing fixed time periods did not allow to detect differences between varieties. Apparently, these were similar in physiological behavior during the vegetative phase independently of growth type.
    La fase de crecimiento vegetativo de la planta de

  17. Estudio etnobotánico del frijol yepatlaxtle (Phaseolus coccineus L., en el área natural protegida Corredor Biológico Chichinautzin, Morelos, México

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    R. Monroy

    2010-01-01

    alimentación y la comercialización de excedentes y aporta ingresos económicos. En otras localidades de la entidad, aún se cultiva en parcelas pequeñas o en el traspatio. El conocimiento tradicional del manejo y uso del frijol es fundamental como alternativa para su conservación; por tanto, se planteó describir el proceso productivo hasta su comercialización y las formas tradicionales de consumo. El proceso productivo del yepataxtle, desde la siembra hasta la cosecha, se registró durante un ciclo agrícola en una parcela cuyo dueño lo explicó. La comercialización se documentó a través de entrevistas a comerciantes y compradores en el mercado municipal durante los días de selecplaza. Las formas de consumo se obtuvieron a través de entrevistas abiertas a las amas de casa que preparan platillos con el yepataxtle. El sistema milpa, cuando incluye yepataxtle, dura 240 días; cuando es solo, 120. Se explican las técnicas campesinas desde la cosmovisión de los informantes. El yepataxtle se comercializa directamente en el mercado, de febrero a mayo como ejote fresco, y en abril, como frijol seco. La información culinaria tradicional del yepataxtle se organizó en un recetario mediante un uso ceremonial para las fiestas patronales del pueblo.

  18. Determinación de la composición físico-química y de la digestibilidad in vitro de dos variedades de frijol común Phaseolus vulgaris L. estimación del contenido de pectina y celulosa en el residuo indigerible

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    Víquez Rodríguez, Floribeth; Bonilla Leiva, Ana Ruth

    2000-01-01

    El propósito de este estudio fue determinar, mediante un estudio de digestibilidad in vitro, la porción indigerible presente en dos variedades de frijol común Phaseolus vulgaris L. consumidas en Costa Rica y cuantificar en dicha porción, dos de los principales polisacáridos no almidonosos causantes de flatulencia: pectina y celulosa. Se determinaron además, las principales características físicas (tamaño y tasa de absorción de agua) y químicas (contenido de proteínas, grasa, cenizas y carbohi...

  19. Efecto de la labranza mínima y la convencional en frijol (phaseolus vulgaris l. En la región Huetar norte de Costa Rica

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    Luis Antonio Rojas

    2002-01-01

    Full Text Available Se realizó un trabajo en la Región Huetar Norte de Costa Rica en donde se establecieron dos lotes: uno de labranza mínima (1,85 ha y otro de labranza convencional (2 ha. La siembra en ambos sistemas de labranza se realizó con una sembradora abonadora hidraúlica. La variedad sembrada fue la Brunca. La población de plantas de frijol evaluada durante la etapa vegetativa fue de 9,37 plantas/m lineal en labranza mínima, y de 9,55 plantas/m lineal en labranza convencional. La densidad de malezas fue mayor en labranza convencional y las malezas de mayor dominancia fueron Ri- chardia scabra, Rottboellia cochinchinensis y Spermacoce sp. El peso seco de malezas fue de 37,6 g de malezas/ cuadrí- cula de 0,25 m2 en labranza mínima, mientras que en el sistema de labranza convencional fue de 46,4 g de malezas/ cuadr ícula. La incidencia y severidad de mustia hilachosa (Tanatheporus cucumeris en el sistema de labranza mínima fue de un 42,8% y de 0,83, respectivamente, mientras que en labranza convencional fue de un 52,2% y 1,22, respectivamente. En el sistema de labranza mínima el rendimiento fue de 930 kg/ha, y en labranza convencional de 676 kg/ha.

  20. (Helianthus annuus L.-frijol (Phaseolus vulgaris L. en función del nitrógeno y fósforo

    Directory of Open Access Journals (Sweden)

    Edgar J. Morales Rosales

    2007-01-01

    Full Text Available En 2003 se realizó un estudio en Montecillo, Estado de México, para determinar el efecto de la aplicación de nitrógeno (0, 40 y 80 kg ha–1 y fósforo (0, 30 y 60 kg P2O5 ha–1 en la siembra combinada de girasol (cv. Victoria y frijol (cv. Michoacán. Con el suministro de 80 kg N ha–1 (80 – 00 – 00 se incrementó la producción de biomasa y rendimiento de semilla superando al testigo sin fertilizante en 32% y 35%. Cuando se adicionaron 60 kg P2O5 ha–1 (00 – 60 – 00 se obtuvo una producción de materia seca y rendimiento de semilla superior al testigo en 24 y 27%, respectivamente. Aunque la interacción nitrógeno x fósforo no fue significativa, existió una tendencia positiva a medida de que se incrementaron los niveles de nitrógeno y fósforo (80 – 60 – 00, alcanzando los máximos valores en la producción de biomasa (2667.9 g m–2 y rendimiento de semilla (644.6 g m–2, los cuales superaron en 49 y 50% al testigo sin fertilizante.

  1. Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L. en Costa Rica

    Directory of Open Access Journals (Sweden)

    Carlos M. Araya

    2000-01-01

    Full Text Available En esta investigación se evaluaron diversos genotipos de frijol en la búsqueda de fuentes de resistencia a la antracnosis (Colletotrichum lindemuthianum y a la mancha angular (Phaeoisariopsis griseola. Se evaluó germoplasma del programa nacional de hibridaciones y de tres viveros internacionales distribuidos por el CIAT: VIFURE, VIPADOGEN y CORE COLLECTION. Los ensayos se establecieron en Puriscal (1017 msnm, Alajuela (814 msnm y Fraijanes (1650 msnm, durante los ciclos agrícolas 97-98 y 98-99. Los materiales se sembraron en parcelas de un surco de dos a seis metros de largo con una o dos repeticiones y manejo con mínimos insumos. Se evaluó la reacción a la infección natural por antracnosis y mancha angular a las seis y ocho semanas después de la siembra, con base en la escala de severidad de uno a nueve. Se seleccionaron materiales con reacción de uno hasta grado tres para antracnosis y de uno hasta grado cuatro para mancha angular. Del grupo de materiales provenientes del programa de hibridaciones se seleccionaron nueve líneas por su resistencia a ambos patógenos. Posteriormente, sólo cinco mostraron resistencia específica a antracnosis y dos a mancha angular. De los viveros distribuidos por el CIAT, el VIPADOGEN aportó dos líneas que mostraron resistencia combinada a las dos enfermedades, mientras que 25 fueron resistentes exclusivamente a antracnosis, además de mostrar tolerancia a baja fertilidad o a sequía. Del vivero CORE COLLECTION, sólo 26 líneas mostraron resistencia combinada; 82 líneas fueron resistentes a antracnosis y 12 a mancha angular. Las mejores fuentes de resistencia a antracnosis y mancha angular estarán disponibles en un vivero internacional

  2. Evaluacion fisicoquimica, microbiologica y sensorial de una salsa y una bebida funcional a base de extracto de frijol rojo (Phaseolus vulgaris con pulpa de Guayaba en el municipio de Valledupar

    Directory of Open Access Journals (Sweden)

    Edwin J Fl

    2017-10-01

    Full Text Available Objetivo: evaluar de manera fisicoquímica, microbiológica y sensorial una salsa y una bebida funcional a base de extracto de fríjol rojo (phaseolus vulgaris con pulpa de guayaba. Metodología: el trabajo se desarrolló en una planta piloto, donde se tuvieron en cuenta la pulpa de la fruta y granos de frijoles rojos comercializados en los diferentes supermercados de la ciudad de Valledupar donde se tomaron muestras representativas de 5 kilos de guayaba y 5 kilos de fríjol para la obtención de la bebida y, con base en la NTC 659, se aplicó un diseño factorial de 22. Resultados: La consistencia de la salsa de extracto de fríjol rojo con mayor contenido de proteína (11,3 % fue el atributo de mayor aceptación por parte de los evaluadores no entrenados con 67,3 %, y el color fue el de menor aceptación con 53,3 %. Sin embargo, en forma general, el producto tuvo una preferencia de 61 %. El sabor de la bebida tipo néctar con mayor contenido de proteína (11% a base de extracto de fríjol rojo y extracto de guayaba fue el atributo que tuvo el mayor porcentaje de aceptación por parte de los evaluadores no entrenados, con 70,6%, y la consistencia fue el atributo con menor porcentaje de aceptación con 61,3. Sin embargo, otra vez en forma general, considerados todos los atributos, la bebida tuvo una preferencia de 64,5%. Conclusiones: la composición mineral (Fe, Na, K, Ca y Mg tanto de la salsa de extracto de fríjol como de la bebida a base de extracto de fríjol rojo y extracto de guayaba fueron aportes valiosos, sobre todo en el contenido del potasio y del calcio, que contribuyen a mantener la estabilidad muscular y gástrica del ser humano.

  3. Microflora en semillas de frijol

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    Jos\\u00E9 B. Membre\\u00F1o

    2001-01-01

    Full Text Available Microflora en semillas de frijol (Phaseolus vulgaris L.. Se estudió la microflora bacteriana presente en semillas de frijol y su relación con Xanthomonas campestris pv. phaseoli (Xcp, en 118 genotipos procedentes de VIDAC-98, INTA- Nicaragua, TARS-USDA e Isabela-P.R. Se utilizaron cinco métodos de aislamiento: semilla desinfectada con hipoclorito de sodio, semilla en caldo nutritivo refrigerada por una hora, dispersión de 0,1 ml de suspensión de semillas en medio sólido, siembra líquida de 1 ml de suspensión y semilla en caldo nutritivo, agitado y refrigerado por 24 horas. Se aislaron 104 colonias amarillas de 41 genotipos. Treinta y seis colonias fueron KOH positivo (Gram negativo, 68 negativo (Gram positivo y 34 hidrolizaron almidón. Las colonias de pigmentación amarilla resultaron no patogénicas bajo condiciones de invernadero. Estas se identificaron con el sistema BIOLOG como: Pantoea agglomerans (25, Xanthomonas campestris (2, Enterobacter agglomerans (2, Sphingomonas paucimobilis (2, Pseudomonas fluorescens y Flavimonas oryzihabitans. En adición, los genotipos portaron colonias con pigmentación distinta a la amarilla. En las pruebas de antagonismo se identificaron colonias con actividad de deoxyribonucleasa y de antibiosis a Xcp. De éstas, 15 colonias inhibieron a Xcp significativamente. Se identificaron los hongos Rhizoctonia solani, Penicillium spp., Fusarium spp., Aspergillus flavus, Rhizopus nigricans y Macrophomina phaseolina en un 52,9 % del total de genotipos evaluados

  4. Gestión de nuevos cultivares de frijol común en las condiciones de la UBPC “Santa Maria 4”

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    Juan Miguel Avila Concepción

    2013-11-01

    Full Text Available Esta investigación se real izó en la UBPC Santa Maria 4 perteneciente a la UEB Antonio Guiteras del municipio Puerto padre, provincia Las Tunas, en el período comprendido entre el 28 de noviembre de 2012 hasta el 16 de febrero de 2013, con el objetivo de evaluar el comportamiento agroproductivo de siete cultivares de frijol común (Phaseolus vulgaris, L. , en un suelo Pardo sialítico mullido carbonatado con vista a incrementar la biodiversidad y elevar el rendimiento agrícola de este cultivo. Los cultivares evaluados fueron (Velazco Largo, Colorado E-120, frijol Rayado, Rojo E-114, Frijol Crema Japones, Rojo E-130, frijol Blanco Español, se utilizó un diseño de bloques al azar con cuatro réplicas, la fitotecnia aplicada fue la tradicional establecida para este cultivo, los aspectos evaluados fueron los siguientes, altura de las plantas, número de hojas, grosor del tallo, altura de la primera vaina, granos por vainas, peso de 100 granos, rendimiento agrícola, incidencia de las principales plagas y valoración económica de la producción. El cultivar que obtuvo mayor rendimiento fue el Velazco Largo y los de menor el Rojo E-114 y el Rayado, los valores obtenidos permiten asegurar que desde el punto de vista económico es factible la realización de estas siembras, pues se pueden obtener incrementos en el ingreso por ventas de $ 6242.90 – 20108.85 pesos por hectárea. En el experimento las condiciones edafoclimáticas fueron favorables en todo el ciclo del cultivo, no se registraron altos niveles de infestación de plagas y enfermedades.

  5. Eficiencia y productividad del cultivo de frijol en un sistema de riego por bombeo en Zacatecas, México

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    José Luis Ríos Flores

    2017-01-01

    Full Text Available La escasez de agua es un factor limitante para la agricultura. Conforme la competencia por este recurso se intensifica, el sector agrícola debe hacer un uso más eficiente. Se estima la productividad del agua en frijol (Phaseolus vulgaris L. producido en Calera y Villa de Cos, Zacatecas, con base en la metodología establecida por Ríos et al. (2015. La productividad física fue 0.118 kg m-3, es decir, 8 442 L kg-1 de frijol para el Distrito de Desarrollo Rural 189 Zacatecas. El precio del metro cúbico para riego fue $0.48 m-3 y utilidad bruta MN$1.88m-3. Se concluye que la productividad agrícola del agua en frijol fue baja, por lo que la producción de frijol en bombeo es insostenible.

  6. Bayo Azteca, primera variedad mejorada de frijol con resistencia a Apion godmani Wagner Bayo Azteca, first improved bean variety with resistance to Apion godmani Wagner

    OpenAIRE

    Ramón Garza-García; Carmen Jacinto-Hernández; Dagoberto Garza-García

    2010-01-01

    Bayo Azteca, la primera variedad mejorada de frijol (Phaseolus vulgaris L.), con resistencia a una plaga insectil, ha sido obtenida por el programa de frijol del Campo Experimental Valle de México, del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias por cruzas múltiples, esto involucró un largo proceso de mejora genética. Bayo Azteca es la primera variedad mejorada resistente al picudo del ejote (Apion godmani Wagner), un curculiónido que ataca al frijol en las zonas t...

  7. FLUJO DE GENES ENTRE FRIJOL COMÚN Y SILVESTRE EN COSTA RICA

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    N\\u00E9stor Chaves-Barrantes

    2009-01-01

    Full Text Available Con el objetivo de analizar el flujo de genes entre frijol común silvestre y cultivado, se determinó el porcentaje de alogamia en condiciones naturales de crecimie nto del frijol silvestre y en estación experimental. En el 2004 se evaluó el cruzamie nto natural entre frijol silvestre mexicano G23511A (Phaseolus vulgaris L. y la línea blanca PAN 68, en la Estación Experimental Fabio Baudrit de la Universidad de Costa Ri ca en Alajuela. Para ello se sembraron dos parcelas experimentales; cada una constó de dos bloques de PAN 68 de 10 surcos (15 m de largo y espacia dos 0,6 m. Entre los bloques y en el borde superior de los mismos, se sembraron tres surcos de frijol silvestre como donantes de polen. Se numeraron las hileras y las plantas de cada una en los bloques de frijol blanco. En Quircot de Ca rtago (área donde crece en forma natural P. vulgaris silvestre, en el 2005 y 2006, se sembraron plantas de frijol comercia l (Vainica de Palo a lo largo de seis sitios junto a las poblaciones de frijol silvestre. En ambas localidades la floración de silvestres y comerciales fue coincidente. Se cosechó toda la semilla de los ensayos y se germinó en bandejas, determinando el porcentaje de alogamia por medio del color morado del hip ocotilo, indicativo de aquellas plántulas cruzadas. En Alajuela el porcentaje de cruza varió entre 0,007 y 0,028 %; mie ntras que en Quircot, entre 0 y 0,199 %.

  8. Morpho-physiological analysis of adaptive responses of common bean (Phaseolus vulgaris L.) to drought stress /

    OpenAIRE

    Polania Perdomo, José A.,

    2016-01-01

    Bibliografia El frijol común (Phaseolus vulgaris L.) es la leguminosa alimenticia más importante en los trópicos. Es cultivada por pequeños agricultores y por lo general expuesta a condiciones desfavorables con mínimo uso de insumos. La sequía y la baja fertilidad del suelo, especialmente deficiencias de nitrógeno (N) y fósforo (P), son principales limitaciones para el rendimiento del frijol en los sistemas de pequeños productores. El frijol puede derivar parte de su requerimiento de N de ...

  9. Variabilidad genética en razas locales de frijol común de Honduras

    OpenAIRE

    Meza Linarez, Narcizo; Rosas Sotomayor, Juan Carlos; Ortiz Marcide, Jesus Maria; Martin Clemente, Juan Pedro

    2010-01-01

    Las regiones agroecológicas Centro-Oriental (Francisco Morazón y El Paraíso) y Nor-Oriental (Olancho) aportan el 52% de la producción de frijol común (Phaseolus vulgaris) en Honduras. En el presente estudio se ha analizado la variabilidad genética en 59 razas locales colectadas en los tres departamentos de las dos regiones agroecológicas mencionadas, mediante cuatro loci microsatélites previamente descritos en P. vulgaris. Los resultados obtenidos muestran la existencia de una gran variabilid...

  10. Estudio de las propiedades fisico químicas y calidad nutricional en distintas variedades de frijol consumidas en México

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    América Fernanda Fernández Valenciano

    2017-01-01

    Full Text Available Introducción : El frijol común ( Phaseolus vulgaris L. es la leguminosa más importante para consumo humano en el mundo , es una fuente importante de proteína, calorías, vitamin as del complejo B y minerales . P o r lo que el objetivo del trabajo de investigación fue estudiar las propiedades f isico químicas y la calidad nutricional en las principales variedades de frijol consumidas y producidas en México, con el propósito de conocer sus beneficios nutricionales para el consumidor. Método : En este estudio se seleccionaron seis variedades de frijol más representativas y consumidas en México : Bayo, Negro, Peruano, Flor de Mayo, Alubia y Pinto, así como, una varieda d de frijol ejotero. A estas variedades se les determin aron las propiedades fisicoquímicas, C, H y N por medio del Analizador Orgánico Elemental Flash 2000. La determinación de Cu, Fe, Zn, Ni, Na, K, Ca y Mg se de terminó por espectrofotometría de absorción atómica y el P se terminó por espectrofotometría y metavandato - molibdato de amonio . Resultados : Se encontraron diferencias significativas en las propiedades f isico químicas, así como, en el contenido de macro y micronutrientes en las diferentes variedades de frijol estudiadas. En general, sobresali ó el frijol ejotero en las propiedades f isico químicas, contenido de micronutrientes y macronutrientes en relación a las variedades de frijol en grano. Discusión o Conclusión : La semilla del ejote presentó las máx imas concentraciones en proteína, N, P, K, Mg, Fe y Zn, mientras que las variedades de frijol de grano estudiadas sobresalieron en fibra (Alubia, proteínas (Pinto y Bayo, en Fe (Flor de Mayo. Por ú ltimo , resaltar que la variedad de frijol de grano estud iada que p resent ó un aporte bajo en proteína, contenido de N, Mg y Ca fue la variedad Frijol Peruano. Finalmente, se concluye que el frijol ejotero representa un gran beneficio para el consumidor por el aporte de prote

  11. Estrategias de mercado para el frijol centroamericano

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    Lourdes Mart\\u00EDnez

    2004-01-01

    Full Text Available Estrategias de mercado para el frijol centroamericano. En los últimos años, el sub-sector de frijol en Centro América ha sido testigo de varios cambios dinámicos. Si no se encuentran medidas para incrementar la competitividad regional de este sub-sector, los países centroamericanos experimentarán impactos negativos en las áreas sociales y económicas de considerable magnitud, especialmente ahora que Centro América necesita ajustar su economía a los nuevos requerimientos de mercados mas abiertos, como el Tratado Centroamericano de Libre Comercio con USA (TLC. Vendedores de frijoles a nivel intermediario y de consumo final, así como técnicos de oficinas gubernamentales dedicadas al mercadeo de frijoles en Centro América fueron entrevistados para documentar los cambios experimentados, identificar los obstáculos que frenan la viabilidad del sub-sector e identificar las oportunidades de incremento de mercados en el futuro para los productores y vendedores de frijol. Los resultados muestran que para que continúen siendo competitivos, los países centroamericanos deben incrementar la productividad a través del apoyo a las investigaciones para el mejoramiento de variedades de frijol, especialmente aquellas que los consumidores prefieren por características específicas (e.g. color. También es imprescindible el fortalecimiento de los programas de distribución de variedades mejoradas a los productores; las mejoras en la transmisión de información de mercado a los productores; el fortalecimiento de los contactos entre productores, comercializadores a nivel mayorista y supermercados; y una política más específica de apertura de mercados de exportación, especialmente con los Estados Unidos.

  12. [Use of Phaseolus vulgaris and Vigna sinensis in a fermented dairy drink].

    Science.gov (United States)

    Granito, Marisela; Trujillo, Lesma; Guerra, Marisa

    2004-06-01

    The objective of this work was to develop a new kind fermented dairy drink, partially substituted with clear varieties of Phaseolus vulgaris (caraota) and Vigna sinensis (frijol). The formulation of fermented dairy drinks included sterile extracts of caraota and frijol, as partial substitutes which replaced milk: 10, 20 and 30%. The mixtures were inoculated with 2% of a mixture of Lactobacillus acidophillus, Streptococcus thermophilus and Bifidobacterium sp. and were incubated at 42 degrees C for 7 hours. Mango and guava jams were used as flavorings at 20%. On the basis of the sensorial evaluation the mixtures 10% frijol-mango, 10% frijol-guava, 30% caraota-mango and 20% caraota-guava were selected. In the selected fermented dairy drinks, the levels of protein, soluble and insoluble fiber, available and resistant starches were increased and the protein digestibility was 81%. The technical feasibility of partial substitution of milk with extracts of Phaseolus vulgaris or Vigna sinensis. For the elaboration of a fermented dairy drink similar to the liquid yogurt kind was demonstrated.

  13. Controles alternativos para el gorgojo del frijol (Acanthoscelides obtectus Say en granos almacenados a partir de materiales naturales y minerales

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    Carlos Ernesto Lopez-Monzon

    2017-03-01

    Full Text Available Con el fin de controlar el daño del gorgojo del frijol [Acanthoscelides obtectus (Say], se evaluó la efectividad en polvo de raíces deshidratadas de chilca (Senecio salignus DC., frutos deshidratados de pimienta negra (Piper nigrum L., fragmentos de madera de pino (Pinus oocarpa Schiede, maíz pulverizado (Zea mays L., cascabillo de frijol (Phaseolus vulgaris L., cal mezclada con ceniza, y arena de río. Se evaluaron ocho tratamientos, con cuatro repeticiones cada uno, en arreglo totalmente al azar. Los materiales naturales y minerales fueron colectados en el municipio de Malacatancito, Huehuetenango y el ensayo se montó en el laboratorio del Centro Universitario de Nor Occidente (Cunoroc, empleando por réplica un frasco de vidrio con capacidad de 1 L y aplicando a cada uno 100 g de semilla de frijol, 10 parejas de gorgojos adultos y 1 g de polvo de los materiales evaluados, según su tratamiento. El análisis se realizó 60 días después de la infestación, donde se evaluó: el porcentaje de daño del gorgojo del frijol, número de gorgojos vivos, correlación del porcentaje de daño versus número de gorgojos vivos y porcentaje de germinación del frijol. El polvo de pimienta negra y la mezcla de cal con ceniza evidenciaron los mejores resultados con el menor porcentaje de daño (1.64% y 3.55%, respectivamente. Las variables porcentaje de daño y número de gorgojos vivos marcaron una fuerte correlación con valor de r = .892. Se estableció que ninguno de los materiales utilizados influyó en la viabilidad de la semilla, registrándose porcentajes de germinación por arriba del 97% en todos los tratamientos. Se recomienda implementar estos productos en el almacenamiento del frijol, para contrarrestar los daños ocasionados por la plaga, obteniendo así beneficios económicos, sociales y ambientales.

  14. Project Phaseolus

    International Nuclear Information System (INIS)

    Anon.

    Research carried out through the Phaseolus Project of the 'Centro de Energia Nuclear na Agricultura' (CENA) Piracicaba, Sao Paulo State, Brazil, is described. It comprises the following subject s: plant breeding; nitrogen fixation; tissue cultures; proteins; photosynthetic efficiency; soil-plant interactions; electron microscopy of the golden mosaic virus; pest control; production of 15 N-enriched ammonium sulfate, and determination of elements in the beans plant. (M.A.) [pt

  15. Colorado

    Directory of Open Access Journals (Sweden)

    Gerardo Rodríguez Quiroz

    2008-01-01

    Full Text Available La conservación de la biodiversidad cuenta, entre sus principales mecanismos de intervención, con las áreas naturales protegidas. En el alto Golfo de California (AGC se ubica la Reser-va de la Biosfera del Alto Golfo de California y Delta del Río Colorado, en la que subsisten especies de alto valor económico, así como especies en peligro de extinción. Este último factor justificó el establecimiento de la reserva. El estudio analiza la efectividad de la Reserva del Alto Golfo como mecanismo de protección de los recursos naturales, en particular de las que están en riesgo de desaparecer, así como de comprobar si los pescadores han mejorado sus condiciones de vida tras la operación de esa área natural. La exploración se llevó a cabo mediante la aplicación de una encuesta a los pescadores. Se sugiere que es indispensable un gran esfuerzo, de autoridades y grupos organizados, para encontrar soluciones al manejo de la Reserva, a fin fijar un programa que permita la recuperación de las especies en peligro de extinción, elevar la calidad de vida de los pescadores y con ello garantizar un equilibrio entre la conservación y la sustentabilidad de la pesca y de los pescadores en el Alto Golfo de California.

  16. BIOMASA Y RENDIMIENTO DE FRIJOL CON POTENCIAL EJOTERO EN UNICULTIVO Y ASOCIADO CON GIRASOL

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    J. Garduño-González

    2009-01-01

    Full Text Available El objetivo del presente estudio fue estimar la producción de biomasa, rendimiento y algunos de sus componentes de dos cultivares de frijol (Phaseolus vulgaris L. con potencial ejotero ('Acerado' y 'Criollo', en unicultivo y asociado con dos cultivares de girasol (Helianthus annuus L. ('Sunbright' y 'Victoria'. El estudio se realizó durante el ciclo primavera verano 2006 bajo condiciones de temporal en Tenancingo, Méx. Las variables evaluadas fueron: índice de área foliar (IAF, tasa de asimilación neta (TAN, biomasa total (BT, diámetro de vaina (DV, longitud de vaina (LV, número de vainas·m-2 (NV y rendimiento de vaina·m-2 (RV; las cuales fueron analizadas en arreglo factorial, bajo un diseño de bloques completos al azar con cuatro repeticiones. Para estimar el grado de asociación, entre las variables estudiadas y el rendimiento de vainas, se realizó un análisis de correlación lineal simple. En el factor cultivares se detectaron diferencias estadísticas significativas para la TAN y DV; para el factor sistemas de siembra hubo diferencias significativas para BT, DV, NV y RV. La interacción cultivares * sistema de siembra fue significativa en todas las variables, excepto en la TAN. En ese sentido, las asociaciones 'Criollo' + 'Sunbright', 'Acerado' + 'Victoria' y 'Acerado' en unicultivo, presentaron el mayor RV. Las variables IAF, BT y NV se correlacionaron positiva y significativamente con el rendimiento de vaina. Los resultados indican que 'Criollo' y 'Acerado', asociados con 'Sunbright' y 'Victoria', respectivamente, responden positivamente a la asociación, constituyendo una buena opción para los productores de la región de Tenancingo, México.

  17. El proyecto "campana educativa para incrementar el consumo de frijoles"

    Directory of Open Access Journals (Sweden)

    Ligia Rodr\\u00EDguez

    2004-01-01

    Full Text Available El proyecto “Campaña educativa para incrementar el consumo de frijoles”. Se describen las diferentes etapas que se siguen en el desarrollo de un proyecto denominado “Campaña educativa para incrementar el consumo de frijoles”. La Campaña tuvo como objetivo “incrementar el consumo de frijoles en la población urbana de clase media de Costa Rica” y contribuir a contrarrestar la disminución en el consumo de este alimento, que se ha presentado en forma muy marcada en el área urbana. El artículo pretende ofrecer una visión global del mencionado proyecto, de manera que le permita al lector la ubicación dentro del proceso, al mismo tiempo que lo refiere a los artículos específicos que ofrecen el detalle de las diferentes etapas y resultados. La metodología describe las características generales del abordaje que se utiliza y la forma en que se desarrolla cada una de las etapas. Los resultados sumarizan los hallazgos de la evaluación y otros aspectos globales del proyecto. Además, ofrece pautas para un abordaje de la educación nutricional focalizada alrededor de un alimento y sustentada en la realidad social y cultural de la población.

  18. Efecto del encalado en la respuesta vegetal y fijación simbiótica del nitrógeno en frijol común

    Directory of Open Access Journals (Sweden)

    Aurelio Garc\\u00EDa

    2003-01-01

    Full Text Available En casa de cristales se evaluó mediante el método de valor A, utilizando el isótopo estable 15N, el efecto del encalado de un suelo Rhodic ferralsol sobre la fijación simbiótica de nitrógeno (FSN de los genotipos de frijol común (Phaseolus vulgaris L. BAT 58, BAT 477, DOR 364, CC 25-9 (N. La isolínea NN 125, genotipo de frijol no nodulante, fue utilizado como cultivo de referencia. El efecto del encalado incrementó la fracción de nitrógeno en planta derivado del aire, principalmente en CC 25-9 (N que posee muy poca capacidad de fijación de N atmosf érico cuando no se encala el suelo. Además, se encontró un efecto beneficioso del encalado sobre las reservas de N del suelo, visto que los incrementos de las extracciones totales de N por las plantas provienen del N del aire, e incluso, la cantidad de N en planta derivado del suelo disminuyó para todos los genotipos, principalmente en DOR 364 y CC 25-9(N. Por otra parte, la relación %Nddf/%Ndds, por ciento de N en planta derivado del fertilizante y por ciento de N en planta derivado del suelo respectivamente, varió debido a la aplicación de cal. Por consiguiente, es necesario utilizar dos tratamientos con el cultivo de referencia para evaluar acertadamente la FSN: cultivo de referencia con y sin suelo encalado. Se discute la interpretación del parámetro Eficiencia de Uso del N.

  19. Fuentes fosfóricas de diferente solubilidad para frijol común evaluadas por método isotópico

    Directory of Open Access Journals (Sweden)

    Aurelio Garc\\u00EDa Altunaga

    2005-01-01

    Full Text Available Se condujeronexperimentos en casa de cristales de la Estación ExperimentalLa Renée ubicada en la provincia Habana en el año 2003. Elobjetivo fue evaluar fuentes fosfóricas de diferente solubilidadpara frijol común (Phaseolus vulgarisL. utilizando dos sue-los, Acrisol y Ferralsol ródico, y la técnica de dilución isotópica con 32P. Las fuentes fosfóricas evaluadas fueron roca fosfórica (RF Trinidad de Guedes, RF parcialmente solubilizadaal 50 % con H2SO4(producto denominado comercialmenteFPA50 y superfosfato simple (SS en el Acrisol y superfosfato triple (SFT en el Ferralsol ródico. Los genotipos de frijol empleados en el Acrisol fueron BAT477, DOR 364, DOR390 y Censa, mientras que en el Ferralsol fueron BAT58, BAT477, DOR 364 y CC-25-9(N. Se evaluó producción de mate-ria seca y extracción de Py se determinó la fracción (% y cantidad de Pen planta derivado de los portadores y % de eficiencia de uso del fertilizante. La RF en aplicación directa fuepoco efectiva mientras que los superfosfatos y el FPA50 resultaron efectivos en ambos suelos. Hubo diferencia varietalen la respuesta vegetal y en la utilización del Pde las fuentes,que también dependió de las características del suelo.

  20. ENCUESTA A PRODUCTORES PARA ORIENTAR EL FITOMEJORAMIENTO DE FRIJOL EN ECUADOR

    Directory of Open Access Journals (Sweden)

    Emmalea Garver Ernest

    2008-01-01

    Full Text Available Se realizó una encuesta a más de cincuenta productores de frijol de los valles de Chota y Mira, ubicados al norte de Ecuador, en el año 2003, con la finalidad de recabar información y plantear objetivos para la implementación de un programa de mejoramiento participativo de frijol en Ecuador. Los productores fueron consultados acerca de las variedades de frijol que están cultivando actualmente, los métodos de producción empleados, los problemas de producción del cultivo y las características que deberían mejorarse en las nuevas variedades. Los principales problemas de producción reportados fueron los daños causados por mosca blanca (Trialeurodes vaporariorum, roya (Uromyces appendiculatus y brúquidos (Acanthoscelides obtectus. Los agricultores han cambiado recientemente las clases comerciales de frijol que tradicionalmente cultivaban en respuesta a la demanda del mercado. En cuanto a las características que deberían poseer las nuevas variedades de frijol, indicaron alto rendimiento y semilla de tamaño grande con color y forma comercial. La costumbre de los agricultores de comprar semilla para cada siembra, y no de producir su propia semilla, fue identifi cada como un obstáculo potencial para el uso y mantenimiento de las nuevas variedades mejoradas de frijol en el área estudiada.

  1. EFECTO DE LA ROTACIÓN DE CULTIVOS EN LA INCIDENCIA DEL AMACHAMIENTO (Aphelenchoides besseyi Christie EN FRIJOL

    Directory of Open Access Journals (Sweden)

    Néstor Felipe Chaves

    2012-01-01

    Full Text Available El efecto de la rotación de cultivos en la incidencia del amachamiento en frijol se evaluó en la región Brunca de Costa Rica durante 2009 y se complementó con una observación de campo en el 2010. La incidencia en 2009 se cuantificó en las etapas de desarrollo vegetativo (V3 o V4, floración (R6 y llenado de vainas (R8 en planta - ciones comerciales de frijol provenientes de rota - ción con arroz, maíz, chile picante, ayote o frijol. En cada plantación se establecieron al azar 4 microparcelas de 10 m 2 , excepto en la secuencia frijol-frijol, donde fueron solo 2; los datos obte - nidos se transformaron angularmente y con un ANDEVA se separaron las medias entre rotacio - nes. La observación de campo del 2010 se llevó a cabo en una finca con 3 parcelas comerciales de frijol en etapa de floración y en suelos previa - mente sembrados con jengibre, tiquizque y maíz, respectivamente; en cada parcela, se establecie - ron sistemáticamente 10 puntos de muestreo para cuantificar la incidencia de amachamiento y se utilizó la prueba de Chi-cuadrado para deter - minar diferencias entre rotaciones. En el 2009, durante la etapa R8 se presentó una incidencia mínima de amachamiento en la rotación chile picante-frijol (4%, niveles intermedios en las rotaciones ayote-frijol (15% y arroz-frijol (29% y altas incidencias en las rotaciones frijol-frijol (62% y maíz-frijol (64%. En la observación de campo del 2010, durante la etapa de floración se presentaron incidencias de 4% en la rotación jengibre-frijol y de 5% en la rotación tiquizque- frijol, que fueron entre 5 y 6 veces menores que la cuantificada en la rotación maíz-frijol (25%. Con base en los resultados obtenidos, el uso de cultivos como chile picante, jengibre, tiquizque y ayote en el ciclo previo a la siembra de frijol, podría contribuir a bajar la incidencia de amacha - miento y las pérdidas que ocasiona en ese cultivo.

  2. CHARACTERISATION OF Phaseolus coccineus INTERSPECIFIC ...

    African Journals Online (AJOL)

    ACSS

    2018-02-20

    Feb 20, 2018 ... genetic base of this crop, especially for adaptation to extreme environments. The runner bean (Phaseolus coccineus) in particular, has been shown to contribute to disease ...... Emerging disease problems: The case of.

  3. CHARACTERISATION OF Phaseolus coccineus INTERSPECIFIC ...

    African Journals Online (AJOL)

    ACSS

    2018-02-20

    Feb 20, 2018 ... habit) and agronomic attributes (plant vigour, days to physiological maturity; DPM and days to 50% flowering; ... Days to flowering and to DPM ranged from 31-39 and 81-86, ...... evolution, and classification in Phaseolus. pp.

  4. Rendimiento de dos variedades mejoradas de frijol, sembrados al voleo y al espeque, en Moss Pam, Waspam, rio Coco

    Directory of Open Access Journals (Sweden)

    Sankara Narvaez Ismael

    2012-10-01

    Full Text Available           Este articulo presenta el comportamiento agronómico del frijol (Phaseolus vulgaris L. en dos variedades:(DOR-364 y H-46, establecidos a través del análisis de dos métodos de siembra (al voleo y al espeque,  para medir la variable rendimiento. El experimento se realizó en la finca académica SNAKY/URACCAN, ubicada en la comunidad Moss Pam, en el municipio de Waspam río Coco, Región Autónoma Atlántico Norte (RAAN. El cultivo de frijol es de gran relevancia en la vida de las comunidades indígenas, ya que constituye al igual que el arroz, el grano de mayor importancia en la dieta familiar. Es de mencionar que las comunidades indígenas de la RAAN utilizan básicamente dos métodos de siembra (al voleo y al espeque; no obstante, siempre los rendimientos son bajos, por lo que se hace necesario el estudio con las condiciones descritas.    Se utilizó un diseño bifactorial en bloques completos al azar, en donde el factor A: equivale a las dos variedades de frijol (DOR-364 y H-46, y el factor B: dos métodos de siembra (al voleo y al espeque. La evaluación se hizo sobre las características morfométricas presentadas en la fase vegetativa y reproductiva del cultivo. El procesamiento de la información se hizo con el programa estadístico InfoStat, indagando desde el análisis del ANAVA y la prueba Kruskal-Wallis. Los resultados mostraron una alta viabilidad germinativa (95%, con diferencias estadísticas entre tratamientos en las variables altura y diámetro en la etapa vegetativa. En la etapa reproductiva las variables: número de vainas/plantas y granos/vainas/plantas, fueron las que presentaron diferencias estadísticas entre tratamientos, contrario a las variables número de flores/plantas y longitud de vainas/plantas que no mostraron diferencias. Finalmente el ANAVA con un 95 por ciento de confiabilidad encontró diferencias de medias en la variable rendimiento.Se recomienda el establecimiento de la variedad H-46

  5. Esfuerzo iberoamericano aporta la secuencia del genoma del frijol al conocimiento mundial.

    OpenAIRE

    Consejo Nacional de Ciencia y Tecnología (CONACYT)

    2012-01-01

    El Proyecto Genoma del-CYTED se plantea como una actividad de cooperación multinacional donde la meta principal del mismo incrementar el conocimiento básico y las herramientas genéticas en este caso apoyar a la plantación de frijol, seleccionando y diseñando nuevas variedades de este grano.

  6. COMPARACIÓN DE LOS COMPONENTES DEL RENDIMIENTO EN VARIEDADES DE FRIJOL EN CONDICIONES DE ACIDEZ Y HUMEDAD RESIDUAL DEL SUELO EN EL SUR DE VERACRUZ

    Directory of Open Access Journals (Sweden)

    Aurelio Morales Rivera

    2015-10-01

    Full Text Available El frijol negro (Phaseolus vulgaris L. se cultiva en suelos ácidos (pH<5 con humedad residual en el sur del estado de Veracruz, México. El cultivo se realiza en unas 6600 ha al final de la temporada de lluvias a mediados de septiembre o principios de octubre. La humedad residual, aunado a la acidez del suelo frecuentemente afectan el periodo de formación de la semilla, reduciendo el rendimiento. El presente trabajo tuvo como objetivo caracterizar variedades de frijol de tipo III en cuanto a su rendimiento de semilla y sus componentes, cultivadas bajo restricciones de humedad residual y acidez del suelo, y altas temperaturas en el ciclo otoño-invierno 2012-2013, en el Municipio de Juan Rodríguez Clara, Veracruz. Se emplearon seis variedades de testa de color negro, seis del tipo ‘Flor de Mayo’ y una del tipo ‘Flor de Junio’. El diseño experimental fue de bloques completos al azar con cuatro repeticiones. La unidad experimental se constituyó de cinco surcos de 5 m de longitud y 0.8 m de separación. Se fertilizó con una dosis de 40-40-00. Las variedades “Negro Veracruz”, “San Andrés”, “Negro Cotaxtla” y “Flor de Junio Marcela”, exhibieron mayor rendimiento de semilla, vainas por m2 y peso de 200 semillas, altura de planta y menor biomasa aérea final que las demás. El mayor rango de variabilidad genética entre las variedades se observó para la biomasa aérea final y altura de planta. La respuesta en rendimiento y sus componentes sugiere que algunas variedades de testa negra han desarrollado mecanismos de tolerancia a la acidez, restricción de humedad residual del suelo y altas temperaturas durante la floración y formación de la semilla, que les permiten producir altos rendimientos a pesar de estar sometidas a estrés ambiental. La variedad de Valles Altos (“Flor de Junio Marcela”, también mostró que podría tener buena adaptación a estas condiciones.

  7. OLEORRESINA DE JÍCAMA Y CALIDAD DE SEMILLA DE FRIJOL INFESTADA CON Acanthoscelides obtectus Say

    Directory of Open Access Journals (Sweden)

    Jos\\u00E9 Antonio Rangel-Lucio

    2011-01-01

    Full Text Available El objetivo de este trabajo fue determinar el efecto de la oleorresina de jícama en la calidad de la semilla de frijol. En laboratorio se obtuvo el extracto de oleorresina media nte HPLC y se detectó la presencia de rotenona (15 mg/l. Se ensayaron tres concentraciones (Ci, g/ml del extracto: C1 (5x10-7, 5x10-6, ¿5x10-2; C2 (1x10-2, 2x10-2,¿6x10-2; C3 (5x10-1, 6x10-1,¿9x10-1 y testigo por concentración, en frascos de 300 ml con 50 g de semilla de frijol (varie dades Flor de Ma yo, Flor de Junio, Ma - yocoba y veinte gorgojos adultos. El ensayo sólo comprendió semillas de frijol tratadas con C2 por 48 horas para evaluar vigor y germinación estándar a los tres y sie te días después de la siembra, bajo normas de IS TA. El aumento de C2 fue gradual en el número de plántulas normales y germinación estándar en el primer recuento, en particular con las diluciones 3 x 10-2 a 6 x 10-2; en el segundo recuento se confirmó la germinación estándar total con estas mismas diluciones. El frijol Ma yocoba en presencia del extracto de semilla de jícama, mantuvo el vigor y valores porcentuales aceptables de germinación estándar de la semilla.

  8. Influence of double cropping on growth and yield of dry beans with colored plastic mulches Influencia del doble cultivo en el crecimiento y rendimiento de frijol con cubierta plástica de colores

    Directory of Open Access Journals (Sweden)

    Luis Ibarra-Jiménez

    2012-12-01

    Full Text Available There are numerous studies of the use of plastic mulches in vegetable production, but there is little documentation of their use with dry beans (Phaseolus vulgaris L. in single and double cropping. The objective of this study was to grow dry beans over two consecutive growing seasons using the same plastic mulch of different colors and examine the influence of soil temperature on growth and yield. The experiment was conducted in Saltillo, Coahuila, Mexico, in the spring and summer of 2008. The treatments included four colored plastic mulches: white-on-black, black, silver-on-black, aluminum-on-black, and bare soil as a control. The experimental design was a randomized complete block with four replicates. The percentage of photosynthetically active radiation (PAR reflected from the plastic tended to be highest with the white-on-black mulch and lowest with the black mulch. Mean soil temperature under the plastic mulch decreased with the increasing percentage of reflected PAR. Mean soil temperature exhibited a relationship of 98% and 99% to yield in the first and second growing season, respectively. Photosynthetically active radiation had a relationship of 98% and 86% to yield in the first and second growing season, respectively. The effect of the colored plastic mulch on yield was significant (p Existen numerosos estudios sobre el uso de cubierta plástica en la producción de hortalizas, sin embargo está muy poco documentado el cultivo simple o doble de frijol (Phaseolus vulgaris L.. El objetivo de este estudio fue cultivar frijol en dos estaciones de crecimiento consecutivas usando la misma cubierta plástica de diferentes colores y examinar la influencia de la temperatura del suelo en el crecimiento y rendimiento de frijol. El estudio fue conducido en Saltillo, Coahuila, México, en la primavera y verano de 2008. Se usaron cuatro colores de acolchado plástico: blanco-sobre-negro, negro, plata-sobre-negro, aluminio-sobre-negro, y control de

  9. Efecto de la rotación de cultivos en la incidencia del Amachamiento (Aphelenchoides besseyi Christie ) en frijol

    OpenAIRE

    Chaves, Néstor Felipe; Araya, Carlos Manuel

    2012-01-01

    El efecto de la rotación de cultivos en la incidencia del amachamiento en frijol se evaluó en la región Brunca de Costa Rica durante 2009 y se complementó con una observación de campo en el 2010. La incidencia en 2009 se cuantificó en las etapas de desarrollo vegetativo (V3 o V4), floración (R6) y llenado de vainas (R8) en plantaciones comerciales de frijol provenientes de rotación con arroz, maíz, chile picante, ayote o frijol. En cada plantación se establecieron al azar 4 microparcelas de 1...

  10. EFECTO DE LA ROTACIÓN DE CULTIVOS EN LA INCIDENCIA DEL AMACHAMIENTO (Aphelenchoides besseyi Christie ) EN FRIJOL

    OpenAIRE

    Néstor Felipe Chaves; Carlos Manuel Araya

    2012-01-01

    El efecto de la rotación de cultivos en la incidencia del amachamiento en frijol se evaluó en la región Brunca de Costa Rica durante 2009 y se complementó con una observación de campo en el 2010. La incidencia en 2009 se cuantificó en las etapas de desarrollo vegetativo (V3 o V4), floración (R6) y llenado de vainas (R8) en planta - ciones comerciales de frijol provenientes de rota - ción con arroz, maíz, chile picante, ayote o frijol. En cada plantación se establecieron al azar 4 microparcela...

  11. Respuesta de frijol al Endospor 33® a dosis 50% de fertilizante nitrogenado/fosfatado en agricultura protegida

    Directory of Open Access Journals (Sweden)

    Juan Manuel Sanchez-Yañez

    2014-01-01

    Full Text Available El frijol requiere fertilización nitrogenada y fosfatada (FNP, cuya indiscriminada aplicación causa pérdida de productividad del suelo, una alternativa de solución para este problema es reducir y optimizar la dosis de FNP con un inoculante mixto con bacterias promotoras de crecimiento vegetal (BPCV y hongos micorricicos vesículo arbusculares (HMA. El objetivo de esta investigación fue analizar la respuesta del frijol al inoculante mixto Endospor 33® a dosis reducida al 50% del FNP. En un suelo ferralítico rojo pobre de Nitrógeno (N y materia orgánica. Con un diseño experimental de bloques al azar con 5 tratamientos y 6 repeticiones. Los resultados indican que el porcentaje de germinación del frijol con Endospor 33® a la dosis 20 mg/semilla fue de 100%. A plántula con 10 mg/planta, tuvo 6,13g de peso fresco total (PFT y de 0,66g de peso seco total (PST comparado con el frijol control relativo (CR con 0,28g de PST con el 100% del FNP. A floración con 30 mg/planta Endospore 33® tuvo 1,5g de PST comparado con 1,07g de PST del frijol CR. A madurez fisiológica con 30mg/planta registro 34,83g/100 semillas, en contraste a su homologo CR con 20,39g/100 semillas. Lo anterior indica que este inoculante mixto es una opción en la producción del frijol a dosis reducida del FNP, sin afectar negativamente su crecimiento y/o rendimiento.

  12. DISTRIBUCIÓN ESPACIAL DEL AMACHAMIENTO DEL FRIJOL (Aphelenchoides besseyi CHRISTIE EN CAMPO

    Directory of Open Access Journals (Sweden)

    N\\u00E9stor Felipe Chaves-Barrantes

    2014-01-01

    Full Text Available El objetivo del presente trabajo fue determinar la distribución espacial del amachamiento en campo. La investigación se realizó entre mayo y julio del 2009 en las comunidades de Veracruz de Pérez Zeledón, San José y Concepción de Buenos Aires, Puntarenas, Costa Rica. Para ello se evaluó la incidencia de la enfermedad por punto de siembra en dos parcelas de frijol provenientes de distintas rotaciones (arroz/frijol y maíz/fri - jol, con lo cual, posteriormente se determinó la distribución espacial de la enfermedad en campo por medio de un análisis de corridas y los índices de varianza/media (V/M, Lloyd y Morisita. En el análisis de corridas se obtuvo un valor pro - medio de Z de -1,06 para la parcela sembrada previamente con arroz, y de -0,98 para la parcela sembrada previamente con maíz, lo que indica una tendencia a la agregación. Los índices de V/M, Lloyd y Morisita fueron mayores que 1 en ambas parcelas, por lo que se determinó que el amacha - miento presentó un patrón espacial agregado (en parches en condiciones de campo. El patrón espacial del amachamiento en campo tiende a enmascararse debido a la rotación con cultivos hospederos de Aphelenchoides besseyi , – su agente causal –, en especial la rotación maíz/frijol donde el produc - tor redistribuye el inóculo de la enfermedad cuando esparce las cañas de maíz sobre el terreno de cultivo

  13. Productividad y adaptación del frijol ejotero en el Valle de México

    Directory of Open Access Journals (Sweden)

    G. Esquivel-Esquivel

    2006-01-01

    Full Text Available Los ejotes son hortalizas de alta calidad nutritiva y en México existe tendencia a incrementar el consumo de esta leguminosa, por lo cual, el objetivo de este trabajo fue identificar variedades de frijol ejotero de diferente hábito de crecimiento, adaptadas al Valle de México con base en la productividad y calidad del ejote en dos fechas de siembra. Se estableció un experimento con 36 variedades de frijol (31 fueron de hábito de crecimiento determinado y 5 de hábito indeterminado, el 21 de mayo y 15 de junio de 2001, en Texcoco, Estado de México. Se registraron los días a floración, días a inicio de corte, días a madurez fisiológica, peso fresco, producción total y longitud de los ejotes, así como el rendimiento de semilla. En la primera fecha de siembra (PFS se evaluó el contenido de proteína y de fibra cruda en los ejotes. Se encontraron diferencias significativas (P≤0.01 para todas las variables cuantificadas. El descenso de la temperatura, el fotoperiodo y la disponibilidad hídrica redujo el número de cortes de ejote, de ocho en la PFS a cinco en la segunda. Las variedades de hábito indeterminado registraron, en promedio, mayor rendimiento total de ejotes y semilla. Ejotero 50, OR 900 y US No. 3 mostraron el mayor rendimiento de ejotes en la PFS y pueden usarse en siembras tempranas. US No. 3, E10-1, F2 y E10-2 sobresalieron en la segunda fecha y pueden utilizarse en siembras tardías. En las dos fechas de siembra, Bayo Alteño mostró resistencia a enfermedades y rendimiento de ejotes y de semilla alto, por lo que puede sembrarse para doble propósito (ejote y grano y como progenitor en el mejoramiento genético del frijol ejotero. El rendimiento y calidad obtenidos mostraron que es factible producir ejotes en el Valle de México

  14. Mejoramiento genético del frijol rojo y negro mesoamericano para Centroamérica y El Caribe

    Directory of Open Access Journals (Sweden)

    Juan C. Rosas

    2000-01-01

    Full Text Available La producción de frijol en Centroamérica es llevada a cabo mayormente en laderas de suelos marginales, limitada por diversos factores bióticos y abióticos. Más de 350,000 t de este grano se producen en cerca de 0,5 millones de hectáreas, con rendimientos promedios de 678 kg/ha. La mayoría de los productores de frijol utilizan bajos insumos y muy pocas fincas están mecanizadas. El autoconsumo es alto; sin embargo, una alta proporción de este grano es comprado por intermediarios. En Honduras, el frijol es el sétimo cultivo en valor económico y es el de mayor rentabilidad entre el maíz, arroz y sorgo. Las dos clases comerciales más importantes en Centroamérica son el frijol rojo y negro pequeños (raza Mesoamérica. El mejoramiento de estos tipos de frijol se ha enfocado en el desarrollo de resistencia a enfermedades (mosaico común, mosaico dorado, antracnosis, mancha angular, roya, mustia hilachosa y bacteriosis común e insectos (principalmente picudo de la vaina; y tolerancia a baja fertilidad, sequía y calor. Programas adecuados de hibridación, evaluación y selección son usados para el desarrollo de cultivares con resistencia múltiple, alto rendimiento, buena adaptación y aceptación comercial. La ampliación de la base genética se obtiene usando fuentes de germoplasma Andino y Mesoamericano en la etapa de hibridación. La selección simultánea por varios caracteres y pruebas multilocalidades son utilizadas. Los viveros y ensayos de adaptación y rendimiento de líneas avanzadas son distribuídos a países de Centroamérica, México, y El Caribe. Las pruebas y validaciones en fincas y la liberación de variedades, son efectuadas por los programas nacionales de frijol de los países miembros de PROFRIJOL, en colaboración con el Zamorano, CIAT y el Bean/Cowpea CRSP

  15. Geohydrology of White Rock Canyon of the Rio Grande from Otowi to Frijoles Canyon

    International Nuclear Information System (INIS)

    Purtymun, W.D.; Peters, R.J.; Owens, J.W.

    1980-12-01

    Twenty-seven springs discharge from the Totavi Lentil and Tesuque Formation in White Rock Canyon. Water generally acquires its chemical characteristics from rock units that comprise the spring aquifer. Twenty-two of the springs are separated into three groups of similar aquifer-related chemical quality. The five remaining springs make up a fourth group with a chemical quality that differs due to localized conditions in the aquifer. Localized conditions may be related to recharge or discharge in or near basalt intrusion or through faults. Streams from Pajarito, Ancho, and Frijoles Canyons discharge into the Rio Grande in White Rock Canyon. The base flow in the streams is from springs. Sanitary effluent in Mortandad Canyon from the treatment plant at White Rock also reaches the Rio Grande

  16. Detección por PCR de Colletotrichum lindemuthianum en cultivos y semillas de frijol en Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Leonardo Martínez Pacheco

    2014-12-01

    Full Text Available Colletotrichum lindemuthianum, agente causal de la antracnosis del frijol, es uno de los patógenos más limitantes en la producción de este cultivo. La detección y correcta identificación de este hongo resulta fundamental para el manejo de la enfermedad, siendo las pruebas moleculares alternativas rápidas y sensibles para este fin. Mediante la técnica de PCR se evaluaron cuatro juegos de cebadores (CY1/CY2, CD1/CD2, ClF4/ITS4 y ClF432/ClR533 para la detección de C. lindemuthianum a partir de tejidos foliares, de vainas y de semillas procedentes de cultivos de frijol de Antioquia, Colombia. Los resultados indicaron que el par CD1/CD2, dirigido al pseudogen de permeasa de hierro Ftr1, fue el más efectivo para detectar el hongo en tejidos y semillas de frijol, así como para identificar aislamientos en cultivos microbiológicos. Para los cebadores CY1/CY2, dirigidos a los ITS del rDNA, se recomienda un esquema de PCR-RFLPs con MseI (=Tru1I para la diferenciación con las especies C. orbiculare y C. trifolii. Estos cebadores generaron resultados consistentes cuando se utilizaron en combinación con ITS1 (ITS1/CY2 e ITS4 (CY1/ITS4. Finalmente, los cebadores ClF4/ITS4 resultaron en amplificaciones inespecíficas y ClF432/ClR533 en fragmentos de difícil resolución en electroforesis de agarosa. Este estudio servirá de apoyo para los programas de certificación de semilla y mejoramiento genético de frijol.

  17. Wild beans (Phaseolus L.) of North America

    Science.gov (United States)

    The wild relatives of the five domesticated species of bean (Phaseolus L.) are widely distributed across the tropics and subtropics of the New World, with taxa extending to the Canadian border, the Caribbean islands and Bermuda, the Galapagos Islands, and south to Argentina. Mesoamerica holds the la...

  18. DETERMINATION OF DEFENSE MECHANISM IN Phaseolus ...

    African Journals Online (AJOL)

    Administrator

    Field studies were conducted to determine the role of defense mechanism in various parameters associated with plant protection subjected to UV-B radiation in Phaseolus trilobus Ait. commonly used as green manure and fodder. Spectrophotometric analysis showed that UV-B radiation decreases the chlorophyll content ...

  19. Mejora genética participativa de la variedad criolla de frijol "Sacapobres"

    Directory of Open Access Journals (Sweden)

    Rodolfo Araya-Villalobos

    2006-01-01

    Full Text Available Agricultores experimentadores de la Región Brunca de Costa Rica solicitaron en el año 1999, al Programa de Investigación y Transferencia de Tecnología Agropecuaria en frijol (PITTA Frijol, la mejora genética de la variedad criolla Sacapobres, una de las principales variedades criollas de la Región Brunca, que posee precocidad (60 días a cosecha, superando en más de diez días a la variedad mejorada más precoz y alto potencial de rendimiento en grano (no había podido ser sustituida por las variedades mejoradas en sus campos de producción, pero de alta emisión de guías y acame. En el Centro Internacional de Agricultura Tropical (CIAT y la Escuela Agrícola Panamericana Zamorano, se efectuaron hibridaciones con esta variedad criolla. La selección de los materiales segregantes se inició en los años 2000 y 2001, en Costa Rica y se basó en la metodología de fitomejoramiento participativo (FP. Los sitios de selección fueron fincas de agricultores y una estación experimental. El principal criterio empleado por los agricultores fue la arquitectura erecta (ausencia o poca emisión de guías y sin acame para facilitar el tránsito por el terreno en las labores de deshierba y fumigación, seguido del tipo, tamaño y color de grano e incidencia de patógenos. Se seleccionaron, en el año 2005, dos líneas promisorias, que pasarán a la etapa de validación. Se describe la estrategia de selección en FP, la genealogía de los progenitores empleados y se concluye sobre el futuro mejoramiento de Sacapobres

  20. Evaluación de la fijación de nitrógeno por cepas de Rhizobium que nodulan Frijol (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    María Inés Ballesteros

    2010-07-01

    Full Text Available La capacidad de fijar nitrógeno de ocho cepas de Rhizohium fue evaluada incdiante dos ensayos de invernadero, en solución nutritiva, con cinco variedades de fríjol: ICA CITARA, ICA PIJAO, DIACOL CATIO, ANDINO y PVA 916. Se determinó en floración el peso seco de la parle aérea, peso seco de nodulos y nitrógeno total en la planta por el método de microkjeldahi. Las cepas de mejor comportamiento en cuanto a nitrógeno fijado fueron en su orden : CIAT 144, CIAT 899, y CIAT 2; aunque en general la respuesta de las cepas fue diferente con la variedad de Iríjol se logró un porcentaje promedio de nitrógeno fijado del orden del 28% comparado con los controles que recibieron nitrógeno combinado (Ensayo 2. Las cepas CIAT 71 15 y CIAT 632 presentaron la menor efectividad con las variedades ensayadas. Se encontró correlación altamente significativa entre el rendimiento en peso seco y el nitrógeno fijado para todas las variedades de fríjol.

  1. Les hybridations interspecifiques dans le genre Phaseolus ...

    African Journals Online (AJOL)

    La réussite de l'introgression de caractères utiles chez le haricot commun P. vulgaris à partir des deux espèces P. coccineus et P. polyanthus dépend en partie des génotypes utilisés. Ce travail vise à identifier des génotypes de Phaseolus compatibles lors des hybridations interspécifiques et à identifier les hybrides issus ...

  2. Onderzoekingen over virusziekten van de boon (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Want, van der J.P.H.

    1954-01-01

    Three viruses were studied which produce diseases in French beans, Phaseolus virus I (PV1), Phaseolus virus 2 (PV2) and a virus isolated from white clover (WKV). Included are symptoms, host plants, properties in vitro, occurrence and spread in the field. Special attention was given to the

  3. The effects of wildfire on the peak streamflow magnitude and frequency, Frijoles and Capulin Canyons, Bandelier National Monument, New Mexico

    Science.gov (United States)

    Veenhuis, J.E.

    2004-01-01

    In June of 1977, the La Mesa fire burned 15,270 acres in and around Frijoles Canyon, Bandelier National Monument and the adjacent Santa Fe National Forest, New Mexico. The Dome fire occurred in April of 1996 in Bandelier National Monument, burned 16,516 acres in Capulin Canyon and the surrounding Dome Wilderness area. Both canyons are characterized by extensive archeological artifacts, which could be threatened by increased runoff and accelerated rates of erosion after a wildfire. The U.S. Geological Survey (USGS) in cooperation with the National Park Service monitored the fires' effects on streamflow in both canyons. Copyright 2004 ASCE.

  4. PRODUCTIVIDAD Y RENTABILIDAD DEL CULTIVO DE FRIJOL CON FERTIRRIEGO EN VERACRUZ, MÉXICO

    Directory of Open Access Journals (Sweden)

    Francisco Javier Ugalde-Acosta

    2011-01-01

    Full Text Available El objetivo de este trabajo fue determinar el efecto del fertirriego en el cultivo de frijol en las zonas centro y sur del estado de Veracruz, México. Durante los ciclos invierno-primavera 2000 y 2001 se evaluaron tres tratamientos: 1 Riego rodado (por gravedad y fertilización sólida manual (testigo regional, con la dosis 40N-40P-0K (RR-40, 2 Riego por goteo y fertilización sólida manual, con la dosis 4N-40P-0K (RG-40; en ambos tratamientos se aplicó el fertilizante a los quince días de nacido el cultivo, y 3 riego por goteo y fertilización soluble, con la dosis 60N-60P-60K, aplicada en forma fraccionada durante el ciclo del cultivo (RG-60. De 2002 a 2005 se validó el mejor tratamiento y se realizó análisis económico para determinar su rentabilidad en comparación con el testigo regional. En la etapa de evaluación, con el tratamiento RG- 60 se redujo en 85% el gasto de agua y se obtuvo el mayor rendimiento promedio (2256 kg/ha, que superó en 145% al obtenido con RG-40 y en 186% al RR-40 (testigo regional. En la etapa de validación con riego por goteo y fertilización soluble fraccionada 60N-60-P-60K se obtuvo 110% más rendimiento que el testigo regional y mejor relación beneficio/ costo (1:1,80.

  5. CARACTERIZACIÓN PRELIMINAR CON MICROSATÉLITES DE LA COLECCIÓN GUATEMALTECA DE FRIJOL COMÚN TREPADOR

    Directory of Open Access Journals (Sweden)

    Karla Melina Ponciano-Samayoa

    2009-01-01

    Full Text Available El objetivo de este estudio fue caracterizar con microsatélites una colección de 558 accesiones de frijol voluble guatemalteco. En el Instituto de Ciencia y Tecnología Agrícolas (ICTA, en el periodo enero 2008 a junio 2009, con base en seis marcadores microsatélites, se analizaron agrupamientos y diversidad genética dentro de los grupos de frijol. Todos los loci analizados fueron polimórficos, se detectaron 25 alelos con un promedio de 4,12 alelos por marcador. El análisis de similaridad detectó genotipos duplicados que al ser eliminados dio lugar a una colección reducida de 261 muestras diferentes (46,8 % de la original. Los análisis de similaridad y correspondencia identificaron 12 grupos, incluyendo un control andino. La diversidad genética de Nei para la colección reducida fue 0,4310, y la diferenciación genética (Gst de 0,5747. La diversidad genética entre los grupos (57,5 % fue mayor que la detectada dentro de los grupos (42,5 %. Las distancias genéticas entre grupos se calcularon en un rango de 0.1566 a 1,6205. Un alto nivel de heterocigosidad fue observado.

  6. Evaluación de líneas de frijol negro, en Veracruz y Chiapas, México

    Directory of Open Access Journals (Sweden)

    Ernesto Lopez

    2001-01-01

    Full Text Available Evaluación de líneas de frijol negro, en Veracruz y Chiapas, México. El presente estudio se llevó a cabo durante los ciclos agrícolas de 1999-2000 y 2000-2001; se evaluó la adaptación y rendimiento de 15 líneas avanzadas de frijol negro, así como un testigo local. Dichos genotipos forman parte al Ensayo Centroamericano de Adaptación y Rendimiento (ECAR. La evaluación se realizó en 11 localidades, cinco en el estado de Chiapas y seis en Veracruz, los experimentos se establecieron bajo condiciones de riego, temporal y humedad residual. Las líneas ICTA-Ju 97-1, ICTA-Ju II- 307, DOR-678 y Cut-45, resultaron sobresalientes por su rendimiento, amplia adaptación y estabilidad. En el ciclo agrícola de otoño-invierno 1999-2000, se evaluó la respuesta de las líneas a las siguientes enfermedades: mosaico dorado (BGMV, mancha angular (Phaseoisariopsis griseola, roya (Uromyces appendiculatus y la antracnosis (Colletotrichum lindemuthianum. Las líneas que resultaron tolerantes fueron ICTA Ju 97-1 y Cut-45. Ambas serán validadas en campos de agricultores para su posible liberación como nuevas variedades para algunas regiones productoras de Veracruz y Chiapas, México

  7. [Microstructural changes in hardened beans (Phaseolus vulgaris)].

    Science.gov (United States)

    Mujica, Maria Virginia; Granito, Marisela; Soto, Naudy

    2015-06-01

    (Phaseolus vulgaris). The hardening of Phaseolus vulgaris beans stored at high temperature and high relative humidity is one of the main constraints for consumption. The objective of this research was to evaluate by scanning electron microscopy, structural changes in cotyledons and testa of the hardened beans. The freshly harvested grains were stored for twelve months under two conditions: 5 ° C-34% RH and 37 ° C-75% RH, in order to promote hardening. The stored raw and cooked grains were lyophilized and fractured. The sections of testa and cotyledons were observed in an electron microscope JSM-6390. After twelve months, grains stored at 37 ° C-75% RH increased their hardness by 503%, whereas there were no significant changes in grains stored at 5 ° C-34% RH. At the microstructural level, the cotyledons of the raw grains show clear differences in appearance of the cell wall, into the intercellular space size and texture matrix protein. There were also differences in compaction of palisade and sub-epidermal layer in the testa of raw grains. After cooking, cotyledon cells of the soft grains were well separated while these ofhard grains were seldom separated. In conclusion, the found differences in hard and soft grains showed a significant participation of both structures, cotyledons and testa, in the grains hardening.

  8. Galactooligosaccharides of seeds during growth of Phaseolus coccineus L. and Phaseolus vulgaris L. beans

    Directory of Open Access Journals (Sweden)

    Ryszard Kosson

    2013-12-01

    Full Text Available The quantitative changes of raffinose and stachyose in seeds of Phaseolus coccineus L. and Phaseolus vulgaris L. during their growth and maturation in a two year experiment were investigated. Trace amounts of raffinose were found in Ph. vulgaris seeds during their vegeta tive growth in 1990. Time of raffinose accumulation in seeds in 1991 began not earlier than on 33rd day since inflorescence. Stachyose started to accumulate in seeds between 33rd and 47th day after inflorescence of all tested cultivars. It was noticed that stachyose and raffinose contents in seeds of most tested cultivars after ten years of storage did not differ significantly when compared to not stored ones.

  9. RELACIONES HÍDRICAS Y TEMPERATURAS ALTAS EN FRIJOL DEL TIPO "FLOR DE MAYO"

    Directory of Open Access Journals (Sweden)

    Edwin Javier Barrios-Gómez

    2011-01-01

    Full Text Available Se estudió el efecto del déficit hídrico del suelo y temperatura alta del aire en las relaciones hídricas y temperatura del dosel del cultivo (Tc, rendimiento de semilla (RS y sus componentes en 8 variedades de frijol del tipo "Flor de Mayo" (FM y una variedad criolla. Se realizaron 3 experimentos de campo; Montecillo en riego (MR con la aplicación de agua de la siembra a la madurez, Montecillo en secano (MS con la lluvia como fuente única de humedad y Celaya en secano (CS con lluvia y 2 riegos suplementarios en floración. Se utilizó una dosis de fertilización de 80 kg.ha-1 de nitrógeno y 40 kg.ha-1 de fósforo. En MR el potencial hídrico foliar ( =-0,33 Mpa, osmótico ( =-1,26 Mpa y de turgencia y (P=0,93 Mpa fue más alto que en CS ( =-0,77, =-1,36 y P=0,59 Mpa y MS ( =-1,22, =-1,57 y P=0,35 Mpa; MS, con mayor deficiencia hídrica del suelo, tuvo mayor reducción en y , y mayor grado de ajuste osmótico (AO=0,70 Mpa. El AO estuvo positiva y significativamente asociado con RS y biomasa aérea final (BMAF en los 2 ambientes de secano. La Tc en MR (26,7°C fue más baja que en CS (30,8°C y MS (36,6°C; el estrés por calor se agudizó con el descenso en el contenido hídrico de las plantas en el ambiente más seco (MS; Tc se relacionó negativa y significativamente con y en MS. La alta Tc también tuvo efecto significativo en la producción de BMAF y RS y sus componentes; la alta Tc redujo el RS, BMAF, número de vainas normales.m-2 (VN.m-2 y peso de 100 semillas (P100S en 12,5, 10,7, 10,2 y 3,4% por cada ºC (-1 ºC de aumento en la Tc, respectivamente.

  10. RESISTENCIA A SEQUÍA DE LINEAS Y VARIEDADES DE FRIJOL NEGRO TROPICAL

    Directory of Open Access Journals (Sweden)

    Ernesto Lopez Salinas

    2011-06-01

    Full Text Available Los objetivos de esta investigación fueron: clasificar genotipos de frijol negro por su tolerancia a sequía e identificar los de mayor eficiencia en el rendimiento en condiciones de riego y sequía. Para ello, en invierno-primavera 2008 se condujeron dos experimentos en Medellín de Bravo, Veracruz; uno con riego todo el ciclo y el otro sin riego, a partir del inicio de floración. Se evaluaron nueve líneas, seis variedades y Criollo Arbolito (testigo local en diseño bloques completos al azar con tres repeticiones. Se cuantificó días a madurez fisiológica, producción de materia seca sin grano, vainas por planta, peso de 100 semillas y rendimiento de grano. Se hicieron análisis de varianza por condición de humedad y uno combinado. Como estimadores de eficiencia se utilizaron el índice de susceptibilidad a sequía (ISS y el índice de eficiencia relativa del rendimiento (IER. Con riego, Negro INIFAP, 82L-17-80, Negro Tacaná y Negro Veracruz, fueron los genotipos más productivos con más de 1,0 t ha-1. Con sequía sobresalieron los dos últimos genotipos, más la línea DOR 448, con rendimientos superiores a 400 kg ha-1. El rendimiento bajo sequía en promedio representó el 37,7% con respecto al de riego. Con ISS de 0,71, 0,85 y 0,87, las líneas DOR-448, NGO 17-99 e Icta Ju-97-1, respectivamente, fueron clasificadas como resistentes a sequía. Las variedades Negro Tacaná y Negro Veracruz con valores de IER de 1,67 y 1,47, mostraron la mayor eficiencia en el rendimiento en ambas condiciones de humedad.

  11. Genetic diversity study of common bean (Phaseolus vulgaris L ...

    African Journals Online (AJOL)

    SAM

    2014-09-03

    Sep 3, 2014 ... Key words: Genetic diversity, ISSR, Phaseolus vulgaris. INTRODUCTION ..... effective germplasm conservation and for setting germ- plasm collection ... conservation and research programs of the species. Furthermore, the ...

  12. Growth Performance of Five Bean (Phaseolus spp) Varieties as ...

    African Journals Online (AJOL)

    PROF HORSFALL

    had significant (P≤ 0.05) effect on bean plant girth, number of leaves, number of branches, mean number of flowers, total fresh ... Beans (Phaseolus spp) belong to one of several genera .... Meng (2016), that found that applying coffee pulp.

  13. Effects of Kidney Bean, Phaseolus vulgaris Meal on the Growth ...

    African Journals Online (AJOL)

    , Oreochromis niloticus (mean weight 1.36 + 0.05 g) fed diets containing varying levels of the kidney bean, Phaseolus vulgaris were investigated under laboratory conditions. The kidney bean was incorporated at separate levels of 60, 40, ...

  14. Interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol

    Directory of Open Access Journals (Sweden)

    Marieta Marín Bruzos

    2013-01-01

    Full Text Available En el estudio se evaluaron, mediante análisis de los parámetros fenológicos de las plantas, la interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol. Se emplearon cuatro tratamientos: plantas sin inocular (control, inoculadas con T. paurometabola C-924, inoculadas con R. leguminosarum biovar phaseoli CFH e inoculadas con ambas cepas. Se observaron diferencias significativas (P < 0.01 en los porcentajes de germinación de las plantas tratadas con microorganismos de forma independiente o conjunta con respecto al control sin inocular. Se determinó que la inoculación de T. paurometabola C-924 afectó el proceso de nodulación de R. leguminosarum biovar phaseoli CFH. Sin embargo, esto no incidió de manera significativa en la altura de las plantas ni en el diámetro del tallo, ya que no se encontraron diferencias entre los tratamientos para estos parámetros. Para el número de hojas, los mejores resultados se obtuvieron con la aplicación de T. paurometabola C-924. Se concluyó que la interacción de T. paurometabola C-924 con R. leguminosarum biovar phaseoli CFH en el frijol estimuló significativamente la germinación de las semillas y el número de hojas de las plantas con respecto al control sin inocular. Aunque la aplicación de T. paurometabola C-924 no favoreció la nodulación de R. leguminosarum biovar phaseoli CFH, esto no afectó las características fenológicas del cultivo.

  15. Validación de genotipos de frijol negro en la zona central del Estado de Veracruz, México

    Directory of Open Access Journals (Sweden)

    Francisco Javier Ugalde Acosta

    2005-01-01

    Full Text Available Validación de genotipos de frijol negro en la zona central del estado de Veracruz, México. El objetivo del presentetrabajo, fue validar genotipos de frijol con el paquete tecnológico del INIFAP, en parcelas de productores. Durante los ciclosde humedad residual de 1998 a 2001 se establecieron nueveparcelas de validación. Los genotipos utilizados fueron: las lí-neas II-307 y DOR-500 y las variedades Negro Huasteco 81,Negro Cotaxtla 91 y Negro INIFAPy como testigo del productor la variedad Jamapa. En las parcelas se realizaron las laboresde manejo agronómico recomendadas por el INIFAPpara esazona de producción. Cada parcela ocupó una superficie total de3000 m2. Se realizaron análisis de varianza individuales por localidad, considerando como repetición los rendimientos obtenidos por cada genotipo (500 m2 por ciclo de evaluación; también se efectuaron análisis combinado con prueba deseparación de medias (DMS 5% y un análisis económico mediante la Tasa de Retorno Marginal. Los resultados indicaronque las variedades y líneas mejoradas fueron superiores en másdel 100% en rendimiento al testigo. El análisis económicomostró que el mayor beneficio económico se obtuvo al sembrarlas variedades Negro INIFAPy Negro Cotaxtla 91, con utilidades superiores a los US $ 286/ha y una relación beneficio/costo de 1,52 y 1,51, respectivamente.

  16. Uso de un vivero móvil para obtener patrones de virulencia de la roya del frijol común

    Directory of Open Access Journals (Sweden)

    James Steadman

    2002-01-01

    Full Text Available Después de identificar nuevas fuentes de resistencia a la roya, causada por Uromyces appendiculatus, es necesario saber si las variedades con nuevos genes conservarán su resistencia cuando sean sembradas en diferentes regiones de producción. Para ello, se pueden utilizar viveros móviles con líneas/variedades de frijol con diferentes genes de resistencia para obtener los patrones de virulencia del patógeno de la roya. El vivero contiene plantas de frijol con seis días después de la germinación. El vivero se coloca por dos a tres horas en un campo de frijol infectado con la roya, y luego se exponen a las plantas bajo neblina por un periodo de 15 horas dentro de un ambiente controlado, para un un período de incubación de ocho a diez días en una casa de malla. En las hojas primarias, se efectúan las lecturas del tamaño de la uredinia (pústulas. Se utilizan las reacciones para evaluar los patrones de virulencia y para predecir estrategias de uso de genes de resistencia. El vivero móvil economiza tiempo y dinero (10-12 días comparado con dos a cuatro meses para identificar patotipos de roya con inoculaciones en el invernadero y permite el conocimiento de los resultados durante el ciclo del cultivo del frijol. El concepto del vivero móvil podría ser especialmente útili en paí- ses donde hay una escasez de invernaderos y puede ser utilizado con otros sistemas de cultivos-patógenos.

  17. Efecto de diferentes distancias de siembra sobre la producción de biomasa del frijol terciopelo (Stizolobium deeringianum en el departamento de Masaya

    Directory of Open Access Journals (Sweden)

    Roberto Tapia Morales

    2002-06-01

    Full Text Available El presente trabajo tuvo como objetivo, evaluar en términos biológicos el efecto de diferentes distancias de siembra entre plantas sobre la producción de biomasa en base verde y seca del frijol terciopelo (Stizolobium deeringianum. Los resultados evidencian que las diferentes distancias de siembra, tuvieron efecto estadísticamente significativo en la producción de biomasa de frijol terciopelo en base verde y seca. Los valores de producción de biomasa más altos en cuanto a producción de biomasa corresponden a aquellos tratamientos Cuya distancias entre planta son iguales a 0.25 m y 0.50 m, obteniéndose 15.3 ton/ha y 12.3 ton/ha en base verde respectivamente y 2.43 ton/ha y 2.025 ton/ha en base seca respectivamente. En lo que respecta a la capacidad de rebrote se demostró que a una altura de corte inferior a 10 cm el frijol terciopelo no es eficiente, ya que en ninguno de los diferentes tratamientos una vez realizado el primer corte se encontraron plantas en estado de rebrote.

  18. Flatulence-causing galactooligosaccharides of Phaseolus coccineus L. and Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ryszard Kosson

    2014-01-01

    Full Text Available Phaseolus coccineus beans were assayed for the presence of raffinose and stachyose in whole seeds and in the morphological parts of the seed: cotyledon, hypocotyl and hull. The quantitative composition of the galactooligosaccharides of Ph. coccineus was compared with Ph. vulgaris seeds. The major galactooligosaccharide of Ph. coccineus seeds is stachyose. Another galactooligosaccharide, verbascose, was present in Ph. coccineus seeds in very low amounts (0.04%. The highest concentrations of raffinose and stachyose were found in the hypocotyl, 1.11% and 7.48%, respectively. The hull contained the lowest quantities of stachyose and only traces of raffinose.

  19. Radiation induced mutations in Phaseolus vulgaris L

    International Nuclear Information System (INIS)

    Al-Rubeai, M.A.F.

    1982-01-01

    A selection of various macro- and micro-mutations was undertaken in the M2 generation of Phaseolus vulgaris cultivars after seed exposure to acute gamma radiation doses of 2.5, 5, 7, 10 and 15 Kr. The chlorophyll mutation was positively correlated with dose. Nevertheless, the highest frequency was at 7 Kr. Several interesting morphological mutants were observed. There were dwarf, stiff stem, shiny small leaf, narrow leaf and green giant mutants. Two selected micromutants were superior in seed yield capacity to their parents. The high yields were related to the high number of pods per plant. In 'The Prince' (seed color: red with beige marbling) several mutants with seeds of black color marbled with beige were selected. These seeds gave M3 segregants exhibiting a range of seed colors including white. Many of these M3 plants were short, early flowering and highly sterile. The work demonstrated that the pigmentation character can readily be changed, and confirmed that the variability induced by radiation can be exploited to obtain desirable mutations. (Author) [pt

  20. AKTIVITAS ENZIM FITASE PADA PERKEMBANGAN KACANG HIJAU (Phaseolus radiatus L

    Directory of Open Access Journals (Sweden)

    Woro Riyadina

    2012-09-01

    Full Text Available This is a study on the phytase enzyme activities on germination of Mung Beans (Phaseolus radiatus L. The activities and stability of phytase enzyme were observed under influence of various incubation temperature (27°C, 37°C and 55"C, and incubation time (1 hour, 2 hours and 3 hours of the Mung Beans (Phaseolus radiatus L in germinating for 1 to 5 days. The results showed that activities of phytase enzyme at the same temperature and incubation time are the same in Mung Beans seed germinating for 1 to 5 days. Phytase enzyme is one of the termostabile enzymes with optimal activities at high temperature.

  1. Colorado Water Institute

    Science.gov (United States)

    Colorado Water Institute Colorado State University header HomeMission StatementGRAD592NewslettersPublications/ReportsCSU Water ExpertsFunding OpportunitiesScholarshipsSubscribeEmploymentAdvisory BoardStaffContact UsCommentsLinks Water Center Logo Water Resources Archive Office of Engagement Ag Water

  2. Insects diversity in lima bean (Phaseolus lunatus

    Directory of Open Access Journals (Sweden)

    WIWIN SETIAWATI

    2005-10-01

    Full Text Available Lima bean (Phaseolus lunatus is a vegetable which usually made as a home yard plant for Indonesian people to fulfill their daily needs. This plant has not been produced in the large number by the farmer. So it is hard to find in the market. Lima bean is light by many kind of insect. Inventory, identification and the study of insect taxon to this plant is being done to collect some information about the insect who life in the plant. The research was done in Balitsa experiment garden in the district of Lembang in Bandung regency on November 2003-February 2004, the experiment start at 4 weeks age, at the height of 1260 m over the sea level. The observation was made systematically by absolute method (D-vac macine and relative method (sweeping net. The research so that there were 26 species of phytofagous insect, 9 species of predator insect, 6 species of parasitoid insect, 4 species of pollinator and 14 species of scavenger insect. According to the research the highest species number was got in the 8th week (3rd sampling, which had 27 variety of species, so the highest diversity was also got in this with 2,113 point. Aphididae and Cicadellidae was the most insect found in roay plant. The research also had high number of species insect so the diversity of insect and evenness become high. A community will have the high stability if it is a long with the high diversity. High evenness in community that has low species dominance and high species number of insect so the high of species richness.

  3. allelopathic effects of eucalyptus tereticornis on phaseolus vulgaris

    African Journals Online (AJOL)

    user

    1. ALLELOPATHIC EFFECTS OF EUCALYPTUS TERETICORNIS ON PHASEOLUS. VULGARIS SEEDLINGS. Sale, F.A.. Department of Forestry and Wildlife, Faculty of ..... Sale, F.A. (2009). Allelopathic influence of Acacia auriculiformis. Eucalyptus citriodora and Gliricidia sepium on germination, growth and yield of millet.

  4. Some engineering properties of white kidney beans (Phaseolus ...

    African Journals Online (AJOL)

    ajl yemi

    2011-12-19

    Dec 19, 2011 ... ... (physical and mechanical) properties, white kidney beans, moisture content, thousand grain mass, static coefficient of friction. INTRODUCTION. White kidney beans (Phaseolus vulgaris L.) are a culti- vated plant grown for fresh and dry consumption and a common raw material in the canned food industry.

  5. Nodulation and nitrogen fixation in common bean (Phaseolus vulgaris)

    African Journals Online (AJOL)

    Mamadou Gueye

    Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment. Ndeye Fatou Diaw GUENE, Adama DIOUF and Mamadou GUEYE*. MIRCEN/ Laboratoire commun de microbiologie IRD-ISRA-UCAD, BP 1386, DAKAR, Senegal. Accepted 23 June 2003.

  6. Phosphorus use efficiency in common bean ( Phaseolus vulgaris L ...

    African Journals Online (AJOL)

    The tripartite symbiosis of common bean (Phaseolus vulgaris L.) recombinant inbred line (RIL) 147 with rhizobia and arbuscular mycorrhizal fungi (AMF) was assessed in sand culture by comparing the effects of three AMF species on the mycorrhizal root colonization, rhizobial nodulation, plant growth and phosphorus use ...

  7. Composite Phaseolus vulgaris plants with transgenic roots as ...

    African Journals Online (AJOL)

    SERVER

    2008-02-19

    Feb 19, 2008 ... ... important processes in the root system will be discussed. Key words: Genetic transformation, Phaseolus vulgaris, Agrobacterium rhizogenes. INTRODUCTION. Grain legumes are important agricultural crops, especially for developing countries, where they provide proteins in vegetarian or meat-poor diets.

  8. Composite Phaseolus vulgaris plants with transgenic roots as ...

    African Journals Online (AJOL)

    Large seeded grain legumes such as the common bean (Phaseolus vulgaris) and cowpea (Vigna unguiculata) are very important crops with seeds that are major protein source for people in developing countries, but their yields and improvement lag behind the economically more important cereals. For research purposes ...

  9. Response of common bean ( Phaseolus vulgaris L.) cultivars to ...

    African Journals Online (AJOL)

    Yield losses in common bean (Phaseolus vulgaris L.) may occur due to boron (B) deficiency when the susceptible cultivars are grown in calcareous boron deficient soils. The study was therefore aimed at investigating the effects of three B doses: control (0.0 kg ha-1), soil application (3.0 kg ha-1) and foliar fertilization (0.3 kg ...

  10. Oriënterend onderzoek over de reuzen- en dwergplanten in F1 en volgende generaties van Phaseolus vulgaris L. x Phaseolus multiflorus Lam.

    NARCIS (Netherlands)

    Geerts, Sipke Johannes

    1949-01-01

    A cross is studied between the selffertilising bush bean (Phaseolus vulgaris L.) "Zeeuwse Bruine Boon" and crossfertilising runner bean (Phaseolus multiflorus Lam.) "stam" (a scarlet flowering stockrunner-bean) or some other (climbing) runners. With the bushbean as mother this cross easily results

  11. Rendimiento de semilla y sus componentes en frijol bajo condiciones de acidez y sequía terminal en el sur de Veracruz.

    OpenAIRE

    Morales Rivera, Aurelio

    2014-01-01

    Se estudió la variabilidad en rendimiento de semilla y sus componentes, y producción de biomasa aérea en un grupo de variedades de frijol de color negro, nativas del sur de Veracruz y un grupo del tipo “Flor de Mayo”, introducidas del Altiplano, y la región central de México, en condiciones de acidez edáfica y sequía terminal en el Municipio de Juan Rodríguez Clara, Veracruz, en el ciclo otoño-invierno 2012-13. Se incluyeron ocho variedades comerciales liberadas por el INIFAP, un criollo de M...

  12. Estadísticos vitales de Bemisia tabaci biotipo B en frijol e interacción con el parasitoide Amitus fuscipennis: Life history parameters and absence of hostparasitoid interaction with Amitus fuscipennis Bemisia tabaci biotype B in bean

    Directory of Open Access Journals (Sweden)

    María del Rosario Manzano Martínez

    2009-10-01

    Full Text Available Se determinaron los estadísticos vitales de la mosca blanca Bemisia tabaci biotipo B en condiciones de laboratorio (26 °C ± 2 °C y 80 ± 5 % de HR en fríjol cultivar ICA-Pijao. Los valores promedios fueron: longevidad 34 ± 1.64 días, fecundidad de 211.4 ± 15.1 huevos/hembra y tasa de oviposición de 6.1 ± 0.28 huevos/día. La tasa neta reproductiva fue de 137.8. El tiempo generacional de 43.8 días y la tasa intrínseca de crecimiento poblacional (r m de 0.125. Estos valores son coincidentes con los reportados en la literatura e indicaron la utilidad del frijol (Phaseolus vulgaris L ICA-Pijao para la multiplicación de B. tabaci. No se logró la reproducción de Amitus fuscipennis en B. tabaci. Estudios de comportamiento mostraron que el parasitoide permaneció solo 617.3 seg en promedio en foliolos infestados con la mosca blanca, la mayor parte del tiempo lo invirtió en caminar (46.4%, limpiarse el cuerpo (35.6% y permanecer quieta (16.7%. A. fuscipennis solo tocó una vez accidentalmente una ninfa de B. tabaci, pero no la examinó con las antenas ni la ovipositó. Los resultados indican que A. fuscipennis no es un enemigo natural potencial de B. tabaci biotipo B.Life-history parameters of the whitefly Bemisia tabaci biotipo B were determined at laboratory conditions at 26 °C ± 2 °C y 80 ± 5 % de RH on bean ICA-Pijao. Mean longevity of the whitefly was 34 ± 1,64 days, mean total fecundity was 211,4 ± 15,1 eggs per female with a daily oviposition rate of 6,1 ± 0,28 eggs per day. Net reproduction rate was 137,8. Generation Time was 43,8 days and the intrinsic rate of population increase r m was 0,125. These values are according to previous ones reported and support usefulness of ICA-Pijao bean for B. tabaci rearing. It was not possible to rear Amitus fuscipennis from B. tabaci. Behaviour studies showed that the parasitoid stayed only average 617,3 s on whitefly infested leaflets. It invested most of the time walking (46

  13. Respuesta fotosintética de algunas variedades de maíz, frijol y café

    Directory of Open Access Journals (Sweden)

    Anundo Polanía

    2009-06-01

    Full Text Available Con el objeto de caracterizar fotosintéticamente algunas variedades de maíz, frijol y café, se estudió el comportamiento de la rata de fotosíntesis neta, de la transpiración y de los diferentes tipos de resistencia foliar a ia difusión y fijación de CO2 en la hoja, frente a las variables temperatura, intensidad de luz y concentración de CO2 en el aire. La variedad de maíz ICA H 507 presentó un comportamiento típico de plantas C 4 con altas ratas de fotosíntesis neta, insaturación de luz y baja fotorespiración. El fríjol Dlacol Andino presentó bajas ratas de fotosíntesis, alta fotorespiración y saturación de luz a bajas Intensidades, características propias de plantas C3. Las dos varíedades de café, Típica y Caturra, presentaron bajas ratas de fotosíntesis, valores de fotorespiración cercanos a los del fríjol, comportamiento típico de plantas C3; no lo es su respuesta a la intensidad de luz, ya que se saturaron a intensidades más altas que las presentadas por plantas C3 reconocidas. Estas varíedades presentaron ratas de fotosíntesis mayores que las observadas por otros autores en plantas de café de más edad. Los resultados obtenidos brindan información útil acerca del comportamiento ecoflslológico de

  14. RENDIMIENTO, CALIDAD NUTRIMENTAL Y RENTABILIDAD DEL FRIJOL EJOTERO DE TEMPORAL EN SAN PABLO IXAYOC, MÉXICO

    Directory of Open Access Journals (Sweden)

    Nicolás Salinas-Ramírez

    2013-01-01

    Full Text Available Una de las estrategias para elevar el rendimiento y la calidad nutricional en las regiones agrícolas es la búsqueda de cultivares con mayor adaptación a diferentes condiciones ambientales. El objetivo de este estudio fue caracterizar cultivares de frijol ejotero con base en su fenología, crecimiento, rendimiento, calidad nutricional y rentabilidad, en clima templado y condiciones de secano. Tres variedades de hábito de crecimiento determinado, ‘Opus’, ‘Strike’ y ‘Black Valentine’, y una de crecimiento indeterminado, ‘Hav-14’, fueron sembradas el 7 de mayo de 2008 en San Pablo Ixayoc, Estado de México, con densidad de 6.25 plantas·m -2 . Se encontraron diferencias en el ciclo biológico desde 90 a 199 días al último corte, así como en rendimiento y número de ejotes. Estas diferencias se relacionaron en mayor grado con la precipitación acumulada (PP, r = 0.83**, la evapotranspiración total del cultivo (ETc, r = 0.71* y unidades térmicas acumu - ladas (UC, r = 0.65*. La variedad Opus mostró el mayor porcentaje de minerales, fósforo, FDA, lignina, FDN y proteína. El ingreso neto más alto se obtuvo con ‘Opus’ y ‘Black Valentine’. No obstante, en un periodo de cinco años (vida media de la espaldera ‘Hav-14’ ofrece mayor ventaja desde el punto de vista económico. Estos resultados sugieren que ‘Hav-14’, ‘Opus’ y ‘Black Valentine’ serían los cultivares más apropiados para lograr mayor producción bajo condiciones de lluvia estacional en el clima templado de San Pablo Ixayoc.

  15. Beans (Phaseolus ssp.) as a Model for Understanding Crop Evolution

    Science.gov (United States)

    Bitocchi, Elena; Rau, Domenico; Bellucci, Elisa; Rodriguez, Monica; Murgia, Maria L.; Gioia, Tania; Santo, Debora; Nanni, Laura; Attene, Giovanna; Papa, Roberto

    2017-01-01

    Here, we aim to provide a comprehensive and up-to-date overview of the most significant outcomes in the literature regarding the origin of Phaseolus genus, the geographical distribution of the wild species, the domestication process, and the wide spread out of the centers of origin. Phaseolus can be considered as a unique model for the study of crop evolution, and in particular, for an understanding of the convergent phenotypic evolution that occurred under domestication. The almost unique situation that characterizes the Phaseolus genus is that five of its ∼70 species have been domesticated (i.e., Phaseolus vulgaris, P. coccineus, P. dumosus, P. acutifolius, and P. lunatus), and in addition, for P. vulgaris and P. lunatus, the wild forms are distributed in both Mesoamerica and South America, where at least two independent and isolated episodes of domestication occurred. Thus, at least seven independent domestication events occurred, which provides the possibility to unravel the genetic basis of the domestication process not only among species of the same genus, but also between gene pools within the same species. Along with this, other interesting features makes Phaseolus crops very useful in the study of evolution, including: (i) their recent divergence, and the high level of collinearity and synteny among their genomes; (ii) their different breeding systems and life history traits, from annual and autogamous, to perennial and allogamous; and (iii) their adaptation to different environments, not only in their centers of origin, but also out of the Americas, following their introduction and wide spread through different countries. In particular for P. vulgaris this resulted in the breaking of the spatial isolation of the Mesoamerican and Andean gene pools, which allowed spontaneous hybridization, thus increasing of the possibility of novel genotypes and phenotypes. This knowledge that is associated to the genetic resources that have been conserved ex situ and in

  16. Beans (Phaseolus spp.) - model food legumes

    International Nuclear Information System (INIS)

    Broughton, W.J.; Hemandez, H.; Blair, M.; Beebe, S.; Gepts, P.; Vanderleyden, J.

    2001-01-01

    Globally, 800 million people are malnourished. Heavily subsidised farmers in rich countries produce sufficient surplus food to feed the hungry, but not at a price the poor can afford. Even donating the rich world's surplus to the poor would not solve the problem. Most poor people earn their living from agriculture, so a deluge of free food would destroy their livelihoods. Thus, the only answer to world hunger is to safeguard and improve the productivity of farmers in poor countries. Diets of subsistence level farmers in Africa and Latin America often contain sufficient carbohydrates (through cassava, corn/maize, rice, wheat, etc.), but are poor in proteins. Dietary proteins can take the form of scarce animal products (eggs, milk, meat, etc.), but are usually derived from legumes (plants of the bean and pea family). Legumes are vital in agriculture as they form associations with bacteria that 'fix-nitrogen' from the air. Effectively this amounts to internal fertilisation and is the main reason that legumes are richer in proteins than all other plants. Thousands of legume species exist but more common beans (Phaseolus vulgaris L.) are eaten than any other. In some countries such as Mexico and Brazil, beans are the primary source of protein in human diets. As half the grain legumes consumed worldwide are common beans, they represent the species of choice for the study of grain legume nutrition. Unfortunately, the yields of common beans are low even by the standards of legumes, and the quality of their seed proteins is sub-optimal. Most probably this results from millennia of selection for stable rather than high yield, and as such, is a problem that can be redressed by modem genetic techniques. We have formed an international consortium called 'Phaseomics' to establish the necessary framework of knowledge and materials that will result in disease-resistant, stress-tolerant, high-quality protein and high-yielding beans. Phaseomics will be instrumental in improving

  17. Colorado Children's Budget 2010

    Science.gov (United States)

    Colorado Children's Campaign, 2010

    2010-01-01

    The "Children's Budget 2010" is intended to be a resource guide for policymakers and advocates who are interested in better understanding how Colorado funds children's programs and services. It attempts to clarify often confusing budget information and describe where the state's investment trends are and where those trends will lead the…

  18. Colorado Children's Budget 2013

    Science.gov (United States)

    Buck, Beverly; Baker, Robin

    2013-01-01

    The "Colorado Children's Budget" presents and analyzes investments and spending trends during the past five state fiscal years on services that benefit children. The "Children's Budget" focuses mainly on state investment and spending, with some analysis of federal investments and spending to provide broader context of state…

  19. Western Slope Colorado

    International Nuclear Information System (INIS)

    Epis, R.C.; Callender, J.F.

    1981-01-01

    A conference on the geology and geologic resources of the Western Slope of western Colorado and eastern Utah is presented. Fourteen papers from the conference have been abstracted and indexed for the Department of Energy's Energy Data Base. These papers covered such topics as uranium resources, oil shale deposits, coal resources, oil and gas resources, and geothermal resources of the area

  20. 78 FR 50095 - Notice of Inventory Completion: History Colorado, Formerly Colorado Historical Society, Denver, CO

    Science.gov (United States)

    2013-08-16

    ... Mountain Reservation, Colorado, New Mexico & Utah may proceed. History Colorado is responsible for....R50000] Notice of Inventory Completion: History Colorado, Formerly Colorado Historical Society, Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. [[Page 50096

  1. Channel and hillslope processes revisited in the Arroyo de los Frijoles watershed near Santa Fe, New Mexico

    Science.gov (United States)

    Gellis, Allen C.; Emmett, William W.; Leopold, Luna Bergere

    2005-01-01

    Detailed documentation of geomorphic changes in the landscape of more than a few years is rarely possible. Channel cross sections, channel profiles, sediment deposition behind dams, and hillslope-erosion plots, originally benchmarked within several watersheds outside Santa Fe, New Mexico, in the 1950’s and 1960’s, for a 1966 report that documented processes and rates of arid-region sediment production and deposition, were resurveyed in the mid-1990’s. Many of the original study sites were relocated and surveyed in the mid-1990’s to determine subsequent channel and hillslope changes and to determine whether trends of channel and hillslope aggradation and degradation that were evident in the 1950’s and 1960’s have continued. In general, the net change in channel geometry has been small over the last 30–40 years. The average change in cross-sectional area of 32 resurveyed cross sections was erosion of 0.27 square meter, which equates to a 4-percent increase in cross-sectional area. The average net change in thalweg elevation for 51 resurveyed cross sections was degradation of 0.04 meter. Unpublished data (1964–68) from the scour chains showed that 371 chains had an average scour of 0.14 ± 0.14 meter and that 372 chains showed an average fill of 0.13 + 0.11 meter. Scour, found in the original study (1958–64) to be proportional to the square root of discharge, was confirmed with the addition of unpublished data (1964–68). The observed channel changes have no consistent trend, compared either to results observed in the original 1966 study or to distance from the watershed divide. The conclusion drawn in the original study was that most channels were aggrading; the resurvey showed that aggradation did not continue.An increase in housing and population in the Arroyo de los Frijoles watershed since the 1950’s has led to more roads. Channel degradation is most noticeable at road crossings. The greatest degradation of the main channel Arroyo de los

  2. Análisis proteómico de la resistencia inducida por micorrización al patógeno foliar Sclerotinia sclerotiorum en frijol (Phaseolus vulgaris l.)

    OpenAIRE

    Quintero Zamora, Edalhí

    2014-01-01

    En los últimos años se han reportado diversos estudios sobre la resistencia inducida por micorrización al ataque de patógenos, en éstos se han determinado los cambios de expresión de genes y proteínas que ocurren en las raíces de plantas colonizadas por hongos micorrízicos arbusculares. Recientemente, se han iniciado estudios para entender cómo afecta esta interacción a la parte aérea de la planta. En nuestro grupo de investigación se llevó a cabo el estudio del efecto de la micorrizaci...

  3. Elongación del hipocótilo en plantas de frijol (phaseolus vulgaris por tratamiento de embriones con mutágenos químicos y físicos.

    Directory of Open Access Journals (Sweden)

    Willy Navarro Álvarez

    2016-03-01

    Full Text Available Plants of common bean were selected with an elongated hipocotyle from M3 population produced through embryos treatment with EMS and gamma rays from Cobalt-60 source. The mutants with elongated hipocotyle were reproduced to know the inheritance of this character.

  4. 76 FR 17444 - Notice of Inventory Completion: Colorado Historical Society (History Colorado), Denver, CO

    Science.gov (United States)

    2011-03-29

    ... Culture, Colorado Historical Society (History Colorado), 1560 Broadway, Suite 400, Denver, CO 80202...: Colorado Historical Society (History Colorado), Denver, CO AGENCY: National Park Service, Interior. ACTION... control of the Colorado Historical Society (History Colorado), Denver, CO. The human remains were removed...

  5. REACCIÓN DE VARIEDADES DE FRIJOL A LA INOCULACIÓN ARTIFICIAL DE Aphelenchoides besseyi, AGENTE CAUSAL DE LA FALSA MANCHA ANGULAR

    Directory of Open Access Journals (Sweden)

    Walter Barrantes-Santamar\\u00EDa

    2008-01-01

    Full Text Available Especímenes adultos y juveniles de A. besseyi provenientes de cultivo in vitro se inocularon sobre el follaje de plantas de frijol de las variedades Bribri, Huetar, Telire y Chirripó Rojo. Las edades de las plantas inoculadas en el invernadero fueron de diez, 20 y 30 días después de siembra (dds. Los bioensayos se realizaron en los invernaderos de la Escuela de Ciencias Agrarias de la Universidad Nacional, Heredia-Costa Rica en el 2006. El objetivo de esta investigación fue evaluar la reacción de variedades de frijol al nematodo A. besseyi. Las variedades estudiadas mostraron similar nivel de resistencia al ataque del nematodo, de acuerdo a la escala de severidad utilizada; sin embargo, las variedades Huetar y Bribri mostraron menor severidad de infección y población de nematodos que las otras dos variedades. Los primeros síntomas de la enfermedad se desarrollaron en las hojas primarias. El período de incubación fue más corto a medida que las inoculaciones se realizaron en plantas de mayor edad. Igualmente, las hojas maduras fueron más susceptibles que las jóvenes a la infección del nematodo. El desarrollo de la enfermedad difi rió entre hojas primarias y trifoliadas y dependió de la disponibilidad de tejido foliar, la madurez del follaje y la producción de inóculo secundario.

  6. Two endornaviruses show differential infection patterns between gene pools of Phaseolus vulgaris.

    Science.gov (United States)

    Khankhum, Surasak; Valverde, Rodrigo A; Pastor-Corrales, Marcial A; Osorno, Juan M; Sabanadzovic, Sead

    2015-04-01

    We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. The two endornaviruses were detected in many genotypes of Mesoamerican origin but rarely in genotypes of Andean origin. The results suggest that these two endornaviruses were introduced into the Mesoamerican modern genotypes during common bean domestication and provide more evidence for the existence of two divergent gene pools of common bean.

  7. Growth and Physiological Responses of Phaseolus Species to Salinity Stress

    Directory of Open Access Journals (Sweden)

    J. S. Bayuelo-Jiménez

    2012-01-01

    Full Text Available This paper reports the changes on growth, photosynthesis, water relations, soluble carbohydrate, and ion accumulation, for two salt-tolerant and two salt-sensitive Phaseolus species grown under increasing salinity (0, 60 and 90 mM NaCl. After 20 days exposure to salt, biomass was reduced in all species to a similar extent (about 56%, with the effect of salinity on relative growth rate (RGR confined largely to the first week. RGR of salt-tolerant species was reduced by salinity due to leaf area ratio (LAR reduction rather than a decline in photosynthetic capacity, whereas unit leaf rate and LAR were the key factors in determining RGR on salt-sensitive species. Photosynthetic rate and stomatal conductance decreased gradually with salinity, showing significant reductions only in salt-sensitive species at the highest salt level. There was little difference between species in the effect of salinity on water relations, as indicated by their positive turgor. Osmotic adjustment occurred in all species and depended on higher K+, Na+, and Cl− accumulation. Despite some changes in soluble carbohydrate accumulation induced by salt stress, no consistent contributions in osmotic adjustment could be found in this study. Therefore, we suggest that tolerance to salt stress is largely unrelated to carbohydrate accumulation in Phaseolus species.

  8. Crop physiological analysis of seed quality variation in common bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Muasya, R.M.

    2001-01-01

    Keywords : Physiological maturity, harvest maturity, earliness, common bean, Phaseolus vulgaris L., morphological markers, variation, moisture content, dry weight, viability, vigour, electrical conductivity, tetrazolium, seed lot, seed

  9. Nutritional analyses for proteins and amino acids in beans (Phaseolus sp.

    Directory of Open Access Journals (Sweden)

    Wathelet B.

    1999-01-01

    Full Text Available The chemical index is a good estimator of seed protein quality of Phaseolus beans. In order to estimate this value, a protein hydrolysis and amino acid quantification are realised. The problems inherent to these techniques are presented.

  10. Compuestos nitrogenados indicadores de estrés en respuesta a las dosis tóxicas y deficientes de Nitrógeno en frijol ejotero

    Directory of Open Access Journals (Sweden)

    Esteban Sánchez

    2016-01-01

    Full Text Available Introducción: Un amplio rango de estreses ambientales, tales como baja temperatura, sequía, alcalinidad, salinidad, deficiencia y toxicidad de nutrientes son potencialmente dañinos para las plantas. El papel del nitrógeno como nutriente esencial y componente estructural de aminoácidos, proteínas, ácidos nucleicos y otros constituyentes esenciales para el desarrollo ha sido ampliamente documentado en varias especies debido a la importancia en los procesos de crecimiento y producción agrícola. Sin embargo, en la actualidad, existe escasa literatura del efecto de la deficiencia y toxicidad de nitrógeno sobre los compuestos osmoreguladores como indicadores de estrés en plantas. Por lo que el objetivo del presente trabajo fue estudiar los compuestos nitrogenados indicadores de estrés (prolina, glicinabetaina y colina en respuesta a las dosis tóxicas y deficientes de N en frijol ejotero desarrollado en cámara de cultivo bajo condiciones controladas y sistema hidropónico. Método: El nitrógeno fue aplicado a la solución nutritiva en la forma de NH4NO3 y en dosis crecientes: N1 = 1.5 mM, N2 = 3.0 mM, N3 = 6.0 mM, N4 = 12.0 mM, N5 = 18.0 mM y N6 = 24.0 mM de N. Los parámetros analizados fueron la acumulación de biomasa, la concentración de prolina, glicinabetaina y colina en hojas, raíces, semillas y vainas de frijol ejotero cv. Strike. Resultados: La aplicación de dosis deficientes y tóxicas de N afectó la producción de biomasa en frijol, siendo las dosis tóxicas las que afectaron más este parámetro. Por otro lado, resaltar que los osmoreguladores prolina, glicinabetaina y colina solamente se acumularon bajo condiciones de toxicidad de N (N6, sin embargo, en condiciones de estrés provocado por la deficiencia de N (N1 no se produce la acumulación de estos compuestos. Discusión o Conclusión: Los compuestos nitrogenados indicadores de estrés solamente se acumulan bajo condiciones de toxicidad de N (N6, sin embargo en

  11. Plant growth and laboratory atmosphere. [Phaseolus multiflorus Willd

    Energy Technology Data Exchange (ETDEWEB)

    Richter, O

    1903-01-01

    The author observed that Phaseolus seedlings grown under glass bell jars which were closed off by water were two or three times as long as those seedlings which were grown under jars without the water closure. It was suspected that coal gas or other impurities were causing these results. Thus, experiments were performed to determine if indeed coal gas was affecting plant growth. Results indicated that coal gas has an inhibiting effect on the growth and length of the seedlings, but it also promotes the growth in thickness. Shortening and thickening was proportional to the concentration of the coal gas and the time of exposure. Mercury vapors were found to produce similar differences in height and thickness of seedlings as coal gas, but they are at the same time lethal to the plants.

  12. Colorado State Capitol Geothermal project

    Energy Technology Data Exchange (ETDEWEB)

    Shepherd, Lance [Colorado Department of Personnel and Adminstration, Denver, CO (United States)

    2016-04-29

    Colorado State Capitol Geothermal Project - Final report is redacted due to space constraints. This project was an innovative large-scale ground-source heat pump (GSHP) project at the Colorado State Capitol in Denver, Colorado. The project employed two large wells on the property. One for pulling water from the aquifer, and another for returning the water to the aquifer, after performing the heat exchange. The two wells can work in either direction. Heat extracted/added to the water via a heat exchanger is used to perform space conditioning in the building.

  13. Navigation Study, Colorado Locks, Colorado River, Matagorda, Texas

    National Research Council Canada - National Science Library

    McCollum, Randy

    2000-01-01

    A 1:70 physical navigation model was built to replicate the Matagorda Locks, approximately one mile of the GIWW east of the locks, approximately one-half mile of the Colorado River north of the GIWW...

  14. Diversidad de razas de colletotrichum lindemuthianum en Antioquía y evaluación de germoplasma de frijol crema-rojo por resistencia a antracnosis

    Directory of Open Access Journals (Sweden)

    Gloria E. Santana

    2002-01-01

    Full Text Available Para verificar si la composición de razas en el Oriente de Antioquía se caracterizaron 15 aislamientos de C. lindemuthianum, usando 12 variedades diferenciales de frijol aceptadas internacionalmente. Se identificaron 11 razas, seis de las cuales (9, 131, 135, 139, 643, y 645 fueron caracterizadas por primera vez. Además, se evaluaron 49 genotipos procedentes de los bancos de germoplasma del CIAT y CORPOICA y cultivares de agricultor de municipios productores. Los genotipos fueron evaluados en campo y en invernadero con las razas más virulentas del patógeno (651 y 653 encontradas en Antioquía. Sólo cuatro genotipos (AND1084, SUG130, LAS106 y DiacolCatio mostraron resistencia a ambas razas. Estos genotipos podrían usarse como parentales para un plan de cruzas, con el fin de introducir genes de resistencia a variedades de importancia comercial que presenten susceptibilidad a la antracnosis.

  15. BLM Colorado Federal Mineral Estate

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This Federal Mineral Estate (Subsurface) dataset is a result of combining data sets that were collected at each BLM Colorado Field Office and using...

  16. BLM Colorado Oil Shale Leases

    Data.gov (United States)

    Department of the Interior — KMZ file Format –This data set contains the Oil Shale Leases for the State of Colorado, derived from Legal Land Descriptions (LLD) contained in the US Bureau of Land...

  17. Análisis de la tecnología local de producción de frijol caraota (Phaseolus vulgaris L. en la zona de vega del rio Arauca Local technology analysis of black bean production (Phaseolus vulgaris L. in area shore of the Arauca river

    Directory of Open Access Journals (Sweden)

    Valderrama N. Yhovana

    1997-12-01

    Full Text Available El cultivo del fríjol negro o caraota constituye un importante renglón agrícola de impacto económico y social en el municipio de Arauquita (Arauca, donde cerca de 500 hectáreas se destinan para su siembra en los mesessecos de Octubre y Noviembre. En este municipio la caraota es un cultivo tradicional, en el cual se emplea poca tecnología y existe poca investigación. El producto se comercializa, principalmente, en Venezuela donde existe una alta demanda. Para analizar la Tecnología Local de Producción (TLP, se realizaron encuestas entre agricultores representativos en diferentes veredas del municipio de Arauquita, encontrándose que es un cultivo en donde la mano de obra es contratada con personal de la región y cuyas labores se resumen en: siembra"a chuzo" sobre terrenos previamente quemados, el control de malezas, y finalmente cosecha y beneficio.
    En la cadena de comercialización entre Colombia y Venezuela existen por lo menos 7 pasos entre el productor y el consumidor final.
    The black bean crop is an important economic and social activity in Arauquita (east of Colombia. In thal region, about 500 hectares are devoted to planting seeds in dry months (October-November. This is a traditional crop, wich used not much technology and there is little research. The product is marketed mainly in Venezuela, where it has high demando This study was carried out making surveys to representative farmers on the region. In the black bean crop the farms work are contracted with personal of the region and its agricultural practices in summary are: burn of the vegetation, sowing, wich is done to random hill ("chuzo", putting several seeds by hill. Except the control of weeds, no fitosanitary control is done, isn't fertilized and its harvest and thresh are manual. Marketing between Colombia and Venezuela has at least seven steps from the producer to final costumer.

  18. Floods in Colorado

    Science.gov (United States)

    Follansbee, Robert; Sawyer, Leon R.

    1948-01-01

    The first records of floods in Colorado antedated the settlement of the State by about 30 years. These were records of floods on the Arkansas and Republican Rivers in 1826. Other floods noted by traders, hunters and emigrants, some of whom were on their way to the Far West, occurred in 1844 on the Arkansas River, and by inference on the South Platte River. Other early floods were those on the Purgatoire, the Lower Arkansas, and the San Juan Rivers about 1859. The most serious flood since settlement began was that on the Arkansas River during June 1921, which caused the loss of about 100 lives and an estimated property loss of $19,000,000. Many floods of lesser magnitude have occurred, and some of these have caused loss of life and very considerable property damage. Topography is the chief factor in determining the location of storms and resulting floods. These occur most frequently on the eastern slope of the Front Range. In the mountains farther west precipitation is insufficient to cause floods except during periods of melting snow, in June. In the southwestern part of the State, where precipitation during periods of melting snow is insufficient to cause floods, the severest floods yet experienced resulted from heavy rains in September 1909 and October 1911. In the eastern foothills region, usually below an altitude of about 7,500 feet and extending for a distance of about 50 miles east of the mountains, is a zone subject to rainfalls of great intensity known as cloudbursts. These cloudbursts are of short duration and are confined to very small areas. At times the intensity is so great as to make breathing difficult for those exposed to a storm. The areas of intense rainfall are so small that Weather Bureau precipitation stations have not been located in them. Local residents, being cloudburst conscious, frequently measure the rainfall in receptacles in their yards, and such records constitute the only source of information regarding the intensity. A flood

  19. Diversity of Rhizobium-Phaseolus vulgaris symbiosis: Overview and perspectives

    International Nuclear Information System (INIS)

    Martinez-Romero, Esperanza

    2001-01-01

    Common bean (Phaseolus vulgaris) has become a cosmopolitan crop, but was originally domesticated in the Americas and has been grown in Latin America for several thousand years. Consequently an enormous diversity of bean nodulating bacteria have developed and in the centers of origin the predominant species in bean nodules is R. etli. In some areas of Latin America, inoculation, which normally promotes nodulation and nitrogen fixation is hampered by the prevalence of native strains. Many other species in addition to R. etli have been found in bean nodules in regions where bean has been introduced. Some of these species such as R. leguminosarum bv. phaseoli, R. gallicum bv. phaseoli and R. giardinii bv. phaseoli might have arisen by acquiring the phaseoli plasmid from R. etli. Others, like R. trap id, are well adapted to acid soils and high temperatures and are good inoculants for bean under these conditions. The large number of rhizobia species capable of nodulating bean supports that bean is a promiscuous host and a diversity of bean-rhizobia interactions exists. Large ranges of dinitrogen fixing capabilities have been documented among bean cultivars and commercial beans have the lowest values among legume crops. Knowledge on bean symbiosis is still incipient but could help to improve bean biological nitrogen fixation. (author)

  20. 78 FR 19296 - Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO

    Science.gov (United States)

    2013-03-29

    ... Reservation, Colorado, New Mexico & Utah agreed to accept disposition of the human remains. In 2006, History....R50000] Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: History Colorado, formerly...

  1. 78 FR 30737 - Irish Potatoes Grown in Colorado; Reestablishment of Membership on the Colorado Potato...

    Science.gov (United States)

    2013-05-23

    ... FR] Irish Potatoes Grown in Colorado; Reestablishment of Membership on the Colorado Potato...: This final rule reestablishes the membership on the Colorado Potato Administrative Committee, Area No... Irish potatoes grown in Colorado. This action modifies the Committee membership structure by amending...

  2. Prácticas de conservación de suelos en sistemas de cultivo maíz-frijol en Cuba

    Directory of Open Access Journals (Sweden)

    Alfonso Claro A.

    2000-01-01

    Full Text Available Se realizaron dos ensayos en segmentos de microcuencas en condiciones de producción de la Provincia de Pinar del Río: uno en Campo Hermoso y el otro en La Comunidad La Leña, Cuba. En ambas localidades se rotó el frijol variedad Bat 304 y Tomeguin 93, seguidos de maíz asociado con Mucuna deeringianum y se establecieron bordes de desagüe protegidos con barreras vivas de Leucaena leucocephala y Gliricidia sepium. Las pérdidas de suelos se estimaron en piscinas de sedimentación y limnígrafos Valday en Campo Hermoso y en el caso de La Leña sólo se determinaron las variaciones de la pendiente y el espesor máximo de sedimentos (EMS al final de cada ciclo. El suelo fue un Ferralítico Cuarcítico (Ultisol, la fertilización se realizó de acuerdo a sus reservas, la preparación y labores culturales con tracción animal. Los resultados al cabo de cinco años mostraron una reducción en las pérdidas de suelos por debajo del umbral permisible (5 t/ha, aumento de la altura de las terrazas debido a los sedimentos acumulados, mayor disponibilidad de agua en la sección receptora, mayor cobertura en el período lluvioso, disminución del efecto erosivo de la lluvia, reciclaje de nutrimentos a través de los abonos verdes e incremento de los rendimientos respecto a las áreas donde no se realizaron prácticas de manejo. Se concluyó que el manejo integrado de los diferentes factores en laderas con pendientes mayores de 5%, es necesario para la conservación de suelos y agua

  3. Adaptación del frijol a suelos de baja fertilidad bajo presión de enfermedades foliares en Costa Rica

    Directory of Open Access Journals (Sweden)

    Rodolfo Araya

    2000-01-01

    Full Text Available El objetivo de este estudio fue identificar germoplasma y líneas mejoradas de alta productividad, en suelos de baja fertilidad y bajo presión de antracnosis y mancha angular. Durante 1996 y 1997 se evaluaron fuentes de tolerancia a la baja fertilidad de diversa procedencia geográfica y con resistencia para otros factores bióticos y abióticos. Se caracterizaron 562 genotipos, 251 mejorados y 311 criollos, en dos localidades de Costa Rica: Fraijanes (1750 msnm; 15,3 °C; 3025 mm; 10º09’ Latitud norte y 84°12’ Longitud oeste y Alajuela (840 msnm, 23,1 °C; 1966 mm; 10°01’ Latitud norte y 84° 16’ Longitud oeste. La densidad de siembra fue de 15 semillas por metro lineal y el tamaño de parcela varió con base en la disponibilidad de semilla; a la siembra sólo se adicionó inoculante de Rhizobium tropici al surco de siembra (4 kg/ha. La línea A321, el testigo local UCR 55, A483, G11640; G19696, G10843 y BAC50 mostraron alto rendimiento en las dos localidades. Los genotipos de alto rendimiento en una localidad fueron: en Alajuela: Guanajuato 132, Negro INIFAP, VAX 2, MAR 3 y en Fraijanes G13860, G92, MOC112, Bayo Alteño, J 117; Negro 8025 y A 247. Se identificó germoplasma no mejorado de diferente acervo genético, procedente de México, Perú y Guatemala, con tolerancia a suelos de baja fertilidad. Esto indica la importancia de evaluar material criollo y del intercambio de germoplasma entre países. Los genotipos superiores serán utilizados en el mejoramiento del frijol común para agricultura de bajo uso de insumos

  4. Colorado Academic Library Master Plan, Spring 1982.

    Science.gov (United States)

    Breivik, Patricia Senn; And Others

    Based on a need to assess current library strengths and weaknesses and to project potential library roles in supporting higher education, this master plan makes a series of recommendations to Colorado's academic libraries. It is noted that the plan was endorsed by both the Colorado Commission on Higher Education and the Colorado State Department…

  5. Diversification and Population Structure in Common Beans (Phaseolus vulgaris L.)

    Science.gov (United States)

    Blair, Matthew W.; Soler, Alvaro; Cortés, Andrés J.

    2012-01-01

    Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild beans and landraces is important for the proper utilization and conservation of these important genetic resources. The goal of this research was to evaluate a collection of wild beans with fluorescent microsatellite or simple sequence repeat markers and to determine the population structure in combination with cultivated beans of all known races. Marker diversity in terms of average number of alleles per marker was high (13) for the combination of 36 markers and 104 wild genotypes that was similar to the average of 14 alleles per marker found for the 606 cultivated genotypes. Diversity in wild beans appears to be somewhat higher than in cultivated beans on a per genotype basis. Five populations or genepools were identified in structure analysis of the wild beans corresponding to segments of the geographical range, including Mesoamerican (Mexican), Guatemalan, Colombian, Ecuadorian-northern Peruvian and Andean (Argentina, Bolivia and Southern Peru). The combined analysis of wild and cultivated accessions showed that the first and last of these genepools were related to the cultivated genepools of the same names and the penultimate was found to be distinct but not ancestral to the others. The Guatemalan genepool was very novel and perhaps related to cultivars of race Guatemala, while the Colombian population was also distinct. Results suggest geographic isolation, founder effects or natural selection could have created the different semi-discrete populations of wild beans and that multiple domestications and introgression were involved in creating the diversity of cultivated beans

  6. Purification and partial characterization of Phaseolus vulgaris seed aminopeptidase

    Directory of Open Access Journals (Sweden)

    Abdala A.P.

    1999-01-01

    Full Text Available The aminopeptidase activity of Phaseolus vulgaris seeds was measured using L-Leu-p-nitroanilide and the L-aminoacyl-ß-naphthylamides of Leu, Ala, Arg and Met. A single peak of aminopeptidase activity on Leu-ß-naphthylamide was eluted at 750 µS after gradient elution chromatography on DEAE-cellulose of the supernatant of a crude seed extract. The effluent containing enzyme activity was applied to a Superdex 200 column and only one peak of aminopeptidase activity was obtained. SDS-polyacrylamide gel electrophoresis (10% presented only one protein band with molecular mass of 31 kDa under reducing and nonreducing conditions. The aminopeptidase has an optimum pH of 7.0 for activity on all substrates tested and the highest Vmax/KM ratio for L-Leu-ß-naphthylamide. The enzyme activity was increased 40% by 0.15 M NaCl, inhibited 94% by 2.0 mM Zn2+, inhibited 91% by sodium p-hydroxymercuribenzoate and inhibited 45% by 0.7 mM o-phenanthroline and 30 µM EDTA. Mercaptoethanol (3.3 mM, dithioerythritol (1.7 mM, Ala, Arg, Leu and Met (70 µM, p-nitroaniline (0.25 mM and ß-naphthylamine (0.53 mM had no effect on enzyme activity when assayed with 0.56 mM of substrate. Bestatin (20 µM inhibited 18% the enzyme activity. The aminopeptidase activity in the seeds decayed 50% after two months when stored at 4oC and room temperature. The enzyme is leucyl aminopeptidase metal- and thiol group-dependent.

  7. Diversification and population structure in common beans (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Matthew W Blair

    Full Text Available Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild beans and landraces is important for the proper utilization and conservation of these important genetic resources. The goal of this research was to evaluate a collection of wild beans with fluorescent microsatellite or simple sequence repeat markers and to determine the population structure in combination with cultivated beans of all known races. Marker diversity in terms of average number of alleles per marker was high (13 for the combination of 36 markers and 104 wild genotypes that was similar to the average of 14 alleles per marker found for the 606 cultivated genotypes. Diversity in wild beans appears to be somewhat higher than in cultivated beans on a per genotype basis. Five populations or genepools were identified in structure analysis of the wild beans corresponding to segments of the geographical range, including Mesoamerican (Mexican, Guatemalan, Colombian, Ecuadorian-northern Peruvian and Andean (Argentina, Bolivia and Southern Peru. The combined analysis of wild and cultivated accessions showed that the first and last of these genepools were related to the cultivated genepools of the same names and the penultimate was found to be distinct but not ancestral to the others. The Guatemalan genepool was very novel and perhaps related to cultivars of race Guatemala, while the Colombian population was also distinct. Results suggest geographic isolation, founder effects or natural selection could have created the different semi-discrete populations of wild beans and that multiple domestications and introgression were involved in creating the diversity of

  8. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection.

    Directory of Open Access Journals (Sweden)

    Paula Rodrigues Oblessuc

    Full Text Available BACKGROUND: The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.. Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. METHODOLOGY AND PRINCIPAL FINDINGS: As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO analysis indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For instance, cytokinin and ethylene responses were up-regulated and jasmonic acid, gibberellin, and abscisic acid responses were down-regulated, indicating that these hormones may play a central role in this pathosystem. Importantly, we have identified putative bean gene orthologs of Arabidopsis genes involved in the plant immune system. Based on experimental validation of gene expression, we propose that hypersensitive reaction as part of effector-triggered immunity may operate, at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes involved in pathogen perception and downstream signaling. CONCLUSIONS/SIGNIFICANCE: We have identified specific bean genes and uncovered metabolic processes and pathways that may be involved in the immune response against pathogens. Our transcriptome database is a rich resource for mining novel defense-related genes, which enabled us to

  9. Bioacoustics of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) on Phaseolus vulgaris (Fabaceae)

    Science.gov (United States)

    Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae), is an economically important pest of common bean Phaseolus vulgaris L. (Fabaceae) in the tropics and subtropics. It is difficult to detect the presence of A. obtectus because the larvae are cryptic and spend most of their developmental time...

  10. Effects of bioprocessed antinutritional factors on bean protein quality : with special emphasis on Phaseolus vulgaris L.

    NARCIS (Netherlands)

    Savelkoul, F.

    1994-01-01

    Legumes, e.g. beans and peas, can contain antinutritional factors. Some varieties of faba beans (Vicia faba), soya beans (Glycine max ) and white kidney beans (Phaseolus vulgaris) can contain in their raw state antinutritional

  11. [Glycemic response to consumption of a cereals and legume (Phaseolus vulgaris) bar on healthy individuals].

    Science.gov (United States)

    Zambrano, Rosaura; Granito, Marisela; Valero, Yolmar

    2013-06-01

    The objective of this work was to formulate a cereals and legume (Phaseolus vulgaris) bar and assess its impact on the glycemic response of healthy individuals, in order to contribute to the healthy food supply beneficial to consumers. A mixture of cereals (corn and oats) and different percentages (20 and 30%) of Phaseolus vulgaris was used to formulate the bar. Additionally, a legume cereal bar without legumes (bar control) was prepared. The bar with 30% of Phaseolus vulgaris was selected through sensory evaluation, being scored with better flavor and texture. This combination of cereals and legumes aminoacid improves complementation and reaches the formulation criteria previously established. Chemical characterization indicated a higher protein content in the bar with 30% of Phaseolus vulgaris (13.55%) relative to the bar control (8.5%). The contents of fat, ash and dietary fiber did not differ between the two bars evaluated. However, the soluble fiber and resistant starch of the selected bar was a 32.05% and 18.67%, respectively, than in the control bar; this may contribute to decreasing the rate of glucose uptake. The selected bar presented a low glycemic index (49) and intermediate glycemic load (12.0) in healthy volunteers, which could lead to a possible reduction in the rate of absorption of glucose into the bloodstream, associated with a carbohydrate content of slow absorption. This bar represents a proposal of a healthy snack for the consumer.

  12. Characterization of Sugar Concentration Among Edible Podded Accessions in the USDA Phaseolus vulgaris Germplasm Collection

    Science.gov (United States)

    The objective is to gain knowledge regarding variation in sugar and flavor content among a sample of dry bean and green pod-type accessions from the USDA Phaseolus Germplasm Core Collection, Pullman, WA. The results could be used to market product quality and offer unique opportunities to expand ma...

  13. Yields and quality of Phaseolus bean cultivars under farmers’ conditions in eastern and southern Africa

    DEFF Research Database (Denmark)

    Jensen, Henning Høgh; Kamalongo, Donwell; Ngwira, Amos

    2014-01-01

    Common bean (Phaseolus vulgaris L.) is a dominant grain legume in eastern and southern Africa, where it constitutes a major source of protein and microminerals in peoples’ diet. The current studies aimed at determining how initially promising genotypes of bean responded in terms of yield and grain...

  14. Visualization of resistance responses in Phaseolus vulgaris using reporter tagged clones of Bean common mosaic virus

    DEFF Research Database (Denmark)

    Naderpour, Masoud; Johansen, Ida Elisabeth

    2011-01-01

    Reporter tagged virus clones can provide detailed information on virus–host interactions. In Phaseolus vulgaris (bean), four recessive and one dominant gene are known to control infection by strains of the potyvirus species Bean common mosaic virus (BCMV). To study the interactions between BCMV...

  15. Formation of adventitious roots on green leaf cuttings of Phaseolus vulgaris L.

    NARCIS (Netherlands)

    Oppenoorth, Johanna Margriet

    1980-01-01

    n this thesis the development of adventitious roots on green leaf cuttings of Phaseolus vulgaris L. is studies. The use of green leaf cuttings has the advantage that the leaf blade provides the developing roots inthe petiole with all the nutrients required, a disadvantage is that the composition of

  16. Cortical projection patterns of magnocellular basal nucleus subdivisions as revealed by anterogradely transported Phaseolus vulgaris leucoagglutinin

    NARCIS (Netherlands)

    Luiten, P.G.M.; Gaykema, R.P.A.; Traber, J.; Spencer Jr., D.G.

    1987-01-01

    The present paper deals with a detailed analysis of cortical projections from the magnocellular basal nucleus (MBN) and horizontal limb of the diagonal band of Broca (HDB) in the rat. The MBN and HDB were injected iontophoretically with the anterograde tracer Phaseolus vulgaris leucoagglutinin

  17. Phaseolus vulgaris leuco-agglutinin immunohistochemistry. A comparison between autoradiographic and lectin tracing of neuronal efferents

    NARCIS (Netherlands)

    Horst, G.J. ter; Karst, H.; Luiten, P.G.M.

    1984-01-01

    The autoradiographic pattern of anterograde labeling as a result from injections with tritiated amino acids is compared to the labeling of efferents with Phaseolus vulgaris leuco-agglutinin after lectin injections in the same nucleus visualized by immunohistochemical methods. This comparison is made

  18. Phenotypic variation in a core collection of common bean (Phaseolus vulgaris L.) in the Netherlands

    NARCIS (Netherlands)

    Zeven, A.C.; Waninge, J.; Hintum, van Th.J.L.; Singh, S.P.

    1999-01-01

    Forty accessions, forming a core collection of mainly bush type of the common bean (Phaseolus vulgaris L.) germplasm in the Netherlands, were evaluated for 14 qualitative and quantitative traits at the Agricultural University, Wageningen (WAU), the Netherlands in 1992. These and an additional 117

  19. Effects of radiation quality on the opening of stomata in intact Phaseolus vulgaris leaves

    International Nuclear Information System (INIS)

    Sikorska, K.; Kozłowska, B.; Ciereszko, I.; Maleszewski, S.

    1997-01-01

    In intact French bean (Phaseolus vulgaris L.) leaves blue radiation enhanced opening of stomata both when it was used individually and when it was used as preirradiation before ''white light'' irradiation. Effects of red radiation were just the contrary

  20. THE ACTION OF UV RADIATION ON MITOTIC INDEX AND MITOTIC DIVISION PHASES AT PHASEOLUS VULGARIS L

    Directory of Open Access Journals (Sweden)

    Csilla Iuliana Bara

    2005-08-01

    Full Text Available In this work, damaging effects of UV radiations on bean Phaseolus vulgaris L. plantule root tips were investigated. Our study proves that by bean plants, the decrease of cell division frequency appears to be part of protection mechanism against especially the short waved UV radiation, with variations depending on cultivar.

  1. Differential proteomics reveals the hallmarks of seed development in common bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Parreira, J R; Bouraada, J; Fitzpatrick, M A; Silvestre, S; Bernardes da Silva, A; Marques da Silva, J; Almeida, A M; Fevereiro, P; Altelaar, A F M; Araújo, S S

    2016-01-01

    Common bean (Phaseolus vulgaris L.) is one of the most consumed staple foods worldwide. Little is known about the molecular mechanisms controlling seed development. This study aims to comprehensively describe proteome dynamics during seed development of common bean. A high-throughput gel-free

  2. BIOCHEMICAL EFFECTS INDUCED BY UV TREATMENT ON 5 ROMANIAN PHASEOLUS VULGARIS L. CULTIVARS, GROWN IN FIELD

    Directory of Open Access Journals (Sweden)

    Iulia Bara

    2006-08-01

    Full Text Available Our study is focused on the influence of the UV-B irradiation, at the level of hidric balance, methabolic activity, and in the content of minerals, polyphenols, pigments, nucleic acids, proteins, of five romanian cultivars: Diva, Star, Vera, Ami, Avans of Phaseolus vulgaris, sawn after germination in enreached UV-B environment in natural field condition.

  3. Proteomic analysis of common bean (Phaseolus vulgaris) by two-dimensional gel electrophoresis and mass spectrometry

    Science.gov (United States)

    The modern cultivated common bean (Phaseolus vulgaris) has evolved from wild common beans distributed in Central America, Mexico and the Andean region of South America. It has been reported that wild common bean accessions have higher levels of protein content than the domesticated dry bean cultiva...

  4. Rhizosphere microorganisms affected by soil solarization and cover cropping in Capsicum annuum and Phaseolus lunatus agroecosystems

    Science.gov (United States)

    Field experiments were conducted to evaluate the effects of soil solarization or cover cropping on bell pepper (Capsicum annuum) and lima bean (Phaseolus lunatus, L.) rhizosphere microorganisms. In Experiment I, flat surface solarization (FSS), raised bed solarization (RBS), cowpea (Vigna unguiculat...

  5. Residential Energy Efficiency Potential: Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, Eric J [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-11-16

    Energy used by Colorado single-family homes that can be saved through cost-effective improvements. Prepared by Eric Wilson and Noel Merket, NREL, and Erin Boyd, U.S. Department of Energy Office of Energy Policy and Systems Analysis.

  6. PIEDRA WILDERNESS STUDY AREA, COLORADO.

    Science.gov (United States)

    Condon, Steven M.; Brown, S. Don

    1984-01-01

    The Pedra Wilderness Study Area, located approximately 30 mi northeast of Durango, Colorado, was evaluated for its mineral-resource potential. Geochemical and geophysical studies indicate little promise for the occurrence of mineral or energy resources in this area. This conclusion is supported by the findings of the earlier study and is suggested by the absence of significant mining activity in the area.

  7. Living with wildfire in Colorado

    Science.gov (United States)

    Patricia A. Champ; Nicholas Flores; Hannah Brenkert-Smith

    2010-01-01

    In this presentation, we describe results of a survey to homeowners living in wildfire-prone areas of two counties along the Front Range of the Rocky Mountains in Colorado. The survey was designed to elicit information on homeowners' experience with wildfire, perceptions of wildfire risk on their property and neighboring properties, mitigation efforts undertaken...

  8. Decay of aspen in Colorado

    Science.gov (United States)

    Ross W. Davidson; Thomas E. Hinds; Frank G. Hawksworth

    1959-01-01

    Quaking aspen (Populus tremuloides Michx.) stands are extensive in the central Rocky Mountains. The species reaches its maximum development in the mountains and high mesas west of the Continental Divide in Colorado (Baker, 1925). On the better sites aspen yields a greater volume of wood in a shorter period than most of the conifers growing at comparable elevations. The...

  9. The impact of local extinction on genetic structure of wild populations of lima beans (Phaseolus lunatus in the Central Valley of Costa Rica: consequences for the conservation of plant genetic resources

    Directory of Open Access Journals (Sweden)

    Daniel Barrantes

    2008-09-01

    : 1023-1041. Epub 2008 September 30.Las poblaciones de plantas pueden experimentar extinción local, y al mismo tiempo, pueden surgir a sus alrededores nuevas poblaciones. Algunas especies pueden colonizar el mismo sitio en múltiples ocasiones. Aquí examinamos el impacto de la extinción local y recolonización en la estructura genética de poblaciones silvestres del frijol lima (Phaseolus lunatus en el valle Central de Costa Rica. Comparamos la diversidad genética de muestras tomadas en poblaciones, antes y después de la extinción, en 13 sitios, usando marcadores de microsatélite. Según los episodios de extinción durante los cinco años previos, clasificamos los sitios así: 1 poblaciones que han experimentado extinción por más de un año, y después han recolonizado (recolonizado, 2 poblaciones que no han experimentado extinción local (control, y 3 poblaciones que no han experimentado extinción local durante el estudio, pero fueron cortadas experimentalmente, simulando una extinción (experimental. Nuestros datos no mostraron una clara tendencia en la variación de las frecuencias alélicas, heterozigosidad, o número efectivo de alelos en y entre grupos de poblaciones. Los niveles de diferenciación genética entre muestras recolectadas en diferentes momentos en el mismo sitio fueron diferentes en los tres grupos de poblaciones. Los sitios recolonizados mostraron el mayor nivel de diferenciación genética (Fst = 0.2769, seguidos de los sitios control (Fst= 0.0576 y sitios experimentales (Fst= 0.0189. Obtuvimos resultados similares en la distancia genética Neis entre muestras (d i,j = 0.1786, 0.0400, y 0.0037, respectivamente. Los cambios genéticos en los frijoles lima dependen de la duración y frecuencia de los episodios de extinción local. Las poblaciones "control" no están en equilibrio. Las implicaciones de estos resultados para el establecimiento de estrategias de conservación de los recursos genéticos de habas se encuentran en discusión.

  10. Adaptación y rendimiento de la variedad de frijol “Negro Medellín” en el sureste de México

    Directory of Open Access Journals (Sweden)

    Ernesto L\\u00F3pez

    2000-01-01

    Full Text Available El presente estudio se llevó a cabo de 1994 a 1997 con el objetivo de evaluar la adaptación y rendimiento de la nueva variedad de frijol “Negro Medellín” en la región del Trópico Húmedo del sureste de México. Se condujeron diez ensayos de rendimiento, cinco en el estado de Veracruz, cuatro en Chiapas y uno en Guerrero. Los ensayos incluyeron 16 genotipos y se realizaron, de acuerdo a las facilidades disponibles en los sitios de prueba, bajo condiciones de humedad residual o de temporal. En las evaluaciones se utilizó un diseño de Bloques Completos al Azar con tres repeticiones. La variedad Negro Medellín resultó sobresaliente por su rendimiento, amplia adaptación, con respuesta superior en ambientes favorables y consistente (1226 kg/ha, bi >1,0, S2di = 0. Durante las evaluaciones, la variedad Negro Medellín resultó tolerante a las enfermedades: virus del mosaico dorado (BGMV, mancha angular (Phaoisariopsis griseola y roya (Uromyces appendiculatus var. appendiculatus, enfermedades de amplia distribución en la región tropical. Sin embargo, en una localidad de Veracruz Negro Medellín resultó susceptible a la antracnosis (Colletotrichum lindemuthianum. Además, la variedad Negro Medellín se validó en parcelas comerciales en campos de agricultores en diez localidades del sureste de México, localidades con clima tropical. En éstas parcelas comerciales, Negro Medellín resultó superior en rendimiento a diversos testigos, variedades criollas y mejoradas, de un 10 a 25%, obteniendo un rendimiento promedio de 1300 kg/ha. La variedad Negro Medellín está en trámite para su registro como nueva variedad para las regiones productoras del sureste de México

  11. Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L. para mapeo genético

    Directory of Open Access Journals (Sweden)

    Roca William M.

    1995-06-01

    Full Text Available

    A small cDNA library from beans (Phaseolus vulgaris L. of about 1500 clones was constructed, to further saturate the bean RFLP linkage map. The primarily synthesized cDNAs were amplified by PCR using the adaptors as primers for amplification (Jepson et al., 1991. Inserts in the range of 500 bp were obtained. 93 clones were singled for further analysis. They showed a degree of repeatibility of around 61 %. Around 80% of the unique clones were single copy, and 20% were low copy sequences as expected from a cDNA library. Three pairs of parental bean lines were chosen for their agronomical traits, and evaluated for polymorphism, which was highest as revealed by digestion with EcoRV (77%, followed by DraI (73%, EcoRI (63% and HindIIl (60%. The highest polymorphism was observed between the pair DOR60 and APNI8, 71% for EcoRV and 57% for EcoRI, respectively. Two clones of the two groups with the most repeated clones were analyzed by slot blot hybridization against the other clones and ribosomal DNA, to understand the origin of the repetitions. Only one clone seemed to be of ribosomal origin, as confirmed by the patterns obtained by hybridization to bean genomic DNA digested with HaelIl, implying that the whole group to which it belonged was of ribosomal origin.  It can be explained by the combined utilization of the PCR amplification methodology and the multipleprimers for the synthesis of the first cDNA strand.

    Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L. de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al., 1991. En ella se utilizan como iniciadores para la reacción los mismos adaptadores empleados para generar los terminales cohesivos del cDNA. Se obtuvieron insertos con un promedio de 500 pares de bases. Se aislaron 93 clones, los

  12. Gunnison, Colorado, subpile study report

    International Nuclear Information System (INIS)

    1994-03-01

    To protect human health and the environment, the UMTRA project will remediate the uranium mill tailings site at Gunnison, Colorado. There are explicit requirements (i.e., 40 Part CFR Part 192) for the surface remediation of radiologically contaminated soils on UMTRA sites. The removal of subpile sediment to the depth required by 40 CFR Part 192 will leave in place deeper foundation sediment that is contaminated with hazardous constituents other than radium-226 and thorium-230. The Department of Energy and the Colorado Department of Health have questioned whether this contaminated soil could potentially act as a continuing source of ground water contamination even after surface remediation based on 40 CFR Part 192 is complete. To evaluate the subpile sediments as a potential source of ground water contamination, the Gunnison subpile study was initiated. This report summarizes the results and findings of this study

  13. Los 'Colorados': Etnohistoria y Toponimia

    NARCIS (Netherlands)

    Gómez-Rendón, J.

    2015-01-01

    Los "colorados" comprendían varios grupos étnicos emparentados etnolingüísticamente que ocupaban el piedemonte andino occidental desde El Carchi hasta Bolívar así como las tierras bajas del Pacífico en los sistemas hidrográficos de los ríos Esmeraldas y Guayas. Aunque la ocupación "colorada" de

  14. Water quality and water rights in Colorado

    International Nuclear Information System (INIS)

    MacDonnell, L.J.

    1989-07-01

    The report begins with a review of early Colorado water quality law. The present state statutory system of water quality protection is summarized. Special attention is given to those provisions of Colorado's water quality law aimed at protecting water rights. The report then addresses several specific issues which involve the relationship between water quality and water use. Finally, recommendations are made for improving Colorado's approach to integrating quality and quantity concerns

  15. 2011 Kids Count in Colorado! The Impact of the Great Recession on Colorado's Children

    Science.gov (United States)

    Colorado Children's Campaign, 2011

    2011-01-01

    "Kids Count in Colorado!" is an annual publication of the Colorado Children's Campaign, which provides the best available state- and county-level data to measure and track the education, health and general well-being of the state's children. "Kids Count in Colorado!" informs policy debates and community discussions, serving as…

  16. 78 FR 72700 - Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO

    Science.gov (United States)

    2013-12-03

    ... Mexico, were invited to consult but did not participate. History and Description of the Remains In the....R50000] Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: History Colorado has completed...

  17. 78 FR 9629 - Irish Potatoes Grown in Colorado; Reestablishment of Membership on the Colorado Potato...

    Science.gov (United States)

    2013-02-11

    ... Service 7 CFR Part 948 [Doc. No. AMS-FV-12-0044; FV12-948-2 PR] Irish Potatoes Grown in Colorado; Reestablishment of Membership on the Colorado Potato Administrative Committee, Area No. 2 AGENCY: Agricultural... membership on the Colorado Potato Administrative Committee, Area No. 2 (Committee). The Committee locally...

  18. Culinary and nutritional quality of Phaseolus vulgaris seeds as affected by environmental factors

    Directory of Open Access Journals (Sweden)

    Kigel J.

    1999-01-01

    Full Text Available Efficient selection for specific culinary and nutritional quality traits needs a better understanding of the genetic and environmental control of quality traits at the structural, physiological and biochemical levels. Field experiments indicate great variability in the Phaseolus gene pool regarding the content of antinutritional compounds, as well as in cooking characteristics of the seeds. These seed attributes are strongly affected by geographic location, edaphic and climatic conditions at site of cultivation. However, information on the influence of specific environmental factors (such as temperature, water availability, edaphic conditions, etc. on seed quality traits, as well as on their stability is very scarce. This lack of knowledge impairs a faster progress in the improvement of Phaseolus seed quality.

  19. Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS mutagenized plants

    Directory of Open Access Journals (Sweden)

    Silué, S.

    2013-01-01

    Full Text Available The aim of this study was to describe the embryos abortion process and the inheritance of the embryos abortion trait in Phaseolus vulgaris plants deficient in seed development. These plants were isolated within the second generation of an ethyl methanesulfonate (EMS TILLING population of P. vulgaris cv. 'BAT93'. Mutant embryos show abnormalities mainly in suspensors, shoot apical meristem (SAM and cotyledons from the globular to the cotyledon stages and abort before maturity compared to those observed in wild-type samples. Mutant embryos show also hyperhydricity and contain low amount of chlorophyll. Genetic analyses of F1, F2 and F3 populations from the crosses carried out between the mutagenized plants with aborting embryos and the wild-type plants indicated that the embryo abortion phenotype is maternally inherited and controlled by a single recessive gene. These Phaseolus mutant plants with aborting embryos constitute a valuable material for plant embryogenesis studies.

  20. Collection, Characterization and Use of Genetic Bariation in Chilean Bean Germplasm (Phaseolus vulgaris L.)

    OpenAIRE

    Bascur B., Gabriel; Tay U., Juan

    2005-01-01

    Recently performed studies on the type of seed protein present from several origins and their morphological traits have shown that the common bean (Phaseolus vulgaris L.) is native to America, being a species without a specific center of origin and with two areas of domestication: Central America and South America. In this last center, three strains were determined, one of them is called "the Chilean strain", which as noted is a sub-center of genetic diversity for this species. With the purpo...

  1. Evaluation of gamma radiation (60-Co) induced mutation in two Phaseolus vulgaris varieties

    International Nuclear Information System (INIS)

    Silva, R.M.

    1984-01-01

    Two varieties of Phaseolus vulgaris (Jutiapan and San Martin) were irradiated at 0, 8, 15, 20 and 30 kR doses in a 60-cobalt gamma source, to identify mutants and 20% lethality. M 2 plants showing morphogical mutations were selected. Differences in sensitivity to irradiation of the two varieties were noted, using data and physiological effects of M 1 . Selection and analysis for protein content were in M 3 as well as hereditary changes. (M.A.C.) [pt

  2. Strategies for improving the nutritional quality of Phaseolus beans through gene engineering

    Directory of Open Access Journals (Sweden)

    Kapila J.

    1999-01-01

    Full Text Available Although Phaseolus species are still difficult to transform, progress in this field now opens the way to engineering beans with a higher nutritional value. The opportunities for gene engineering in nutritional quality improvement, the strategies which canbe adopted and the constraints we are still facing are briefly outlined, using the enhancement of the seed methionine content and the reduction in antinutritional factors as examples.

  3. Failure of survival strategies in adaption of heavy metal environment in lens culinaris and phaseolus mungo

    International Nuclear Information System (INIS)

    Haider, S.; Azmat, R.

    2012-01-01

    Lead (Pb)-treated Lens culinaris and Phaseolus mungo seedlings leaves showed considerable reduction in the size with enhance proline and phenol contents while peroxidase and lignin activity was Pb/sup 2+/ dose dependent. The reduced leaves sizes of both seedlings were correlated with an increase in Pb/sup 2+/levels, and activities of peroxidase and lignin deposition in it. The intensification of activities of peroxidase and phenol in the Pb/sup 2+/ treated plants were accompanied by an increase in the biosynthesis of the lignin contents as their function is of scavenging ROS radical. A strong correlation (r/sup 2/=0.8570) was observed between Pb/sup 2+/ and lignin deposition in the Lens culinaris whereas it was non-significant in Phaseolus mungo (r/sup 2/=0.466). Increased in the lignin contents in the Lens culinaris as a chemical adaptation of the cell walls of various leaves tissues for endurance while decrease in the lignin contents in Phaseolus mungo at high dose of Pb/sup 2+/ may be attributed with the decline in the peroxidase activity. Investigations revealed that although plants adopt several biochemical strategies for their survival but toxicity of Pb/sup 2+/was significant due to which plant fails to continue in stay alive. (author)

  4. 40 CFR 81.406 - Colorado.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Colorado. 81.406 Section 81.406 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Visibility Is an Important Value § 81.406 Colorado. Area name Acreage Public Law establishing Federal land...

  5. 2013 Kids Count in Colorado! Community Matters

    Science.gov (United States)

    Colorado Children's Campaign, 2013

    2013-01-01

    "Kids Count in Colorado!" is an annual publication of the Children's Campaign, providing state and county level data on child well-being factors including child health, education, and economic status. Since its first release 20 years ago, "Kids Count in Colorado!" has become the most trusted source for data and information on…

  6. EFECTO DEL MOMENTO DE LA SIEMBRA DEL FRIJOL TERCIOPELO (MUCUNA SP. COMO CULTIVO DE COBERTERA EN EL RENDIMIENTO DE MAÃ?Z

    Directory of Open Access Journals (Sweden)

    José Bernardino Castillo-Caamal

    2010-09-01

    Full Text Available La agricultura milpera se practica desde hace casi tres milenios en el área maya mesoamericana. Se basa en el manejo de la vegetación con barbecho prolongado; y, el cultivo breve del maíz y sus acompañantes. Actualmente, debido a una mayor presión sobre los recursos, el barbecho se ha reducido, en consecuencia también el rendimiento de grano de maíz, atribuido a la disminución de la fertilidad y aumento de las arvenses. Los cultivos de cobertera contribuyen a resolver algunos de los problemas asociados a la milpa. Sin embargo, se conoce poco del funcionamiento de estas, en condiciones de baja precipitación y suelos permeables. Así, la fecha de siembra del frijol terciopelo (FT estas plantas juega un papel fundamental en el manejo de la biomasa y en el cultivo principal. Por lo tanto, el objetivo de este trabajo fue evaluar los tratamientos siguientes: 1 control, maíz sin FT, 2 siembra simultánea de FT con el maíz (0 DDSM, 3 siembra del FT 20 días después del maíz (20 DDSM, 4 siembra del FT 40 días después del maíz (40 DDSM y 5 siembra del FT 60 días después del maíz (60 DDSM. Se utilizó un diseño experimental de cuadrado latino 5 x 5 x 5, se registraron las variables siguientes: rendimiento de grano de maíz y sus componentes de rendimiento, contenidos de nitrógeno, fósforo y potasio del suelo en la fase masosa del grano de maíz; y, contenidos de nitrógeno, fósforo y potasio en el grano de maíz, vaina y grano del FT. La siembra simultánea y 20 DDSM del FT redujeron el rendimiento de grano del maíz en 59 y 25 %, respectivamente. El rendimiento de grano de maíz se ajustó a la ecuación y=-0.5525X2+48.54X+606.3 (R2=0.96, mientras que el rastrojo a y=-0.648X2+65.632X+3989.7 (R2=0.99. El nitrógeno del suelo se redujo en la siembra simultánea (P

  7. Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. under different soil moisture levels near Nairobi, Kenya

    NARCIS (Netherlands)

    Muniafu, M.M.; Macharia, J.N.M.; Stigter, C.J.; Coulson, G.L.

    1999-01-01

    Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. cv GLP-2 under two soil moisture levels in two contrasting seasons near Nairobi, Kenya were investigated. The experiment confirms that dry weights and yields of Phaseolus vulgaris are

  8. Effect of variable protein contents in diets containing Phaseolus vulgaris beans on performance, organ weights and blood variables in piglets, rats and chickens

    NARCIS (Netherlands)

    Huisman, J.; Poel, A.F.B. van der; Mouwen, J.M.V.M.; Weerden, E.J. van

    1990-01-01

    A comparison was made of the effects of antinutritional factors present in Phaseolus vulgaris on piglets, rats and chickens. Also the hypothesis of whether the negative effect on weight gain due to the inclusion of raw Phaseolus vulgaris in the diet can be attributed to an insufficient supply of

  9. Hepatoprotective and antioxidant activity of Phaseolus trilobus, Ait on bile duct ligation induced liver fibrosis in rats.

    Science.gov (United States)

    Fursule, R A; Patil, S D

    2010-06-16

    Phaseolus trilobus Ait (Fabaceae) is extensively used by tribal people of Nandurbar district (Maharashtra, India) in the treatment of Jaundice and other liver disorders. of the present study was to assess the medicinal claim of Phaseolus trilobus as hepatoprotective and antioxidant. The hepatoprotective activity of methanol and aqueous extract of Phaseolus trilobus was evaluated by bile duct ligation induced liver fibrosis and antioxidant activity was evaluated using in vitro and in vivo antioxidant models viz anti-lipid peroxidation assay, super oxide radical scavenging assay and glutathione estimation in liver. Liver function tests were carried out to detect hepatoprotective activity, which was further supported by histopathological examination. Methanol and aqueous extracts of Phaseolus trilobus reduced elevated level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), bilirubin and hydroxyproline significantly (pWistar rats, proving hepatoprotective activity comparable with Silymarin. Both the extracts were found to reduce the elevated levels of serum thiobarbituric acid reactive substance (TBARS) and elevate superoxide scavenging radical activity proving antioxidant activity comparable with ascorbic acid. The reduced level of glutathione was found to be elevated in liver proving antioxidant activity comparable with Silymarin. Phaseolus trilobus posses hepatoprotective property and is effective in oxidative stress induced cholestatic hepatic injury. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

  10. 7 CFR 948.51 - Colorado Potato Committee.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Colorado Potato Committee. 948.51 Section 948.51... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE IRISH POTATOES GROWN IN COLORADO Order Regulating Handling Committees § 948.51 Colorado Potato Committee. The Colorado Potato Committee...

  11. Colorado's prospectus on uranium milling

    International Nuclear Information System (INIS)

    Hazle, A.J.; Franz, G.A.; Gamewell, R.

    1982-01-01

    The first part of this paper will discuss Colorado's control of uranium mill tailings under Titles I and II of the Uranium Mill Tailings Radiation Control Act of 1978. Colorado has a legacy of nine inactive mill sites requiring reclamation under Title I, and two presently active plus a number of new mill proposals which must be regulated in accordance with Title II. Past failures in siting and control on the part of federal jurisdictions have left the state with a heavy legacy requiring extensive effort to address impacts to the state's environment and population. The second part of this paper will discuss the remedial action programme authorized under Public Law 92-314 for Mesa Country, where lack of federal control led to the dispersal of several hundred thousand tons of uranium mill tailings on thousands of properties, including hundreds of homes, schools and other structures. Successful completion of the State efforts under both programmes will depend on a high level of funding and on the maintenance of adequate regulatory standards. (author)

  12. Energy Smart Colorado, Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gitchell, John M. [Program Administrator; Palmer, Adam L. [Program Manager

    2014-03-31

    Energy Smart Colorado is an energy efficiency program established in 2011 in the central mountain region of Colorado. The program was funded through a grant of $4.9 million, awarded in August 2010 by the U.S. Department of Energy’s Better Buildings Program. As primary grant recipient, Eagle County coordinated program activities, managed the budget, and reported results. Eagle County staff worked closely with local community education and outreach partner Eagle Valley Alliance for Sustainability (now Walking Mountains Science Center) to engage residents in the program. Sub-recipients Pitkin County and Gunnison County assigned local implementation of the program in their regions to their respective community efficiency organizations, Community Office for Resource Efficiency (CORE) in Pitkin County, and Office for Resource Efficiency (ORE) in Gunnison County. Utility partners contributed $166,600 to support Home Energy Assessments for their customers. Program staff opened Energy Resource Centers, engaged a network of qualified contractors, developed a work-flow, an enrollment website, a loan program, and a data management system to track results.

  13. Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus

    Directory of Open Access Journals (Sweden)

    Baudoin JP.

    1998-01-01

    Full Text Available Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzymes. Thirty-six haplotypes are identified on the basis of the polymorphism in fragment number and size. Three main phylogenetic groups, strongly supported through bootstrap analysis, are identified: (1 accessions from Phaseolus lunatus and Phaseolus xolocotzii; (2 accessions from Phaseolus glabellus; (3 accessions from Phaseolus vulgaris, Phaseolus polyanthus and Phaseolus coccineus. Within the third group, accessions of Phaseolus coccineus are scattered along the phylogenetic tree, which provides some evidence that coccineus accessions are paraphyletic with respect to Phaseolus vulgaris and Phaseolus polyanthus. An analysis of molecular variance applied on four species show that they are significantly differentiated with 79% of molecular variance among species and 21% within species. The results agree with previous investigations on chloroplast DNA variation in the genus Phaseolus, and suggest that PCRRFLP methods, which are technically less labour-intensive than previous methods, are of great value for phylogenetic investigations at the generic level.

  14. Physiological traits of endornavirus-infected and endornavirus-free common bean (Phaseolus vulgaris) cv Black Turtle Soup.

    Science.gov (United States)

    Khankhum, S; Valverde, R A

    2018-04-01

    This study evaluated the physiological traits of eight lines of common bean (Phaseolus vulgaris) cv. Black Turtle Soup, four of which were double-infected with Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, and four of which were endornavirus-free. Plants from all eight lines were morphologically similar and did not show statistically significant differences in plant height, wet weight, number of days to flowering and pod formation, pods per plant, pod thickness, seed size, number of seeds per pod, and anthocyanin content. However, the endornavirus-infected lines had faster seed germination, longer radicle, lower chlorophyll content, higher carotene content, longer pods, and higher weight of 100 seeds, all of which were statistically significant. The endornaviruses were not associated with visible pathogenic effects.

  15. Influence of 60Co gamma radiation on Zabrotes subfasciatus (Bohemann, 1833) (Coleoptera, Curculionidae) and beams Phaseolus vulgaris

    International Nuclear Information System (INIS)

    Moraes Rego, A.F. de; Rodrigues, Z.A.; Oliveira, M.L. de; Santana, M.D.

    1986-01-01

    Influence of gamma radiation on Zabrotes subfasciatus (Boh, 1833) (Coleoptera Bruchidae) and the beans Phaseolus vulgaris (L.). The effects of 60 CO gamma radiation, 50 Gy, on both Phaseolus vulgaris (L.) seedbeans and adults of Zabrotes subfasciatus were studied using the no free choise method. Radiation decreased insect fertility hence insect population and it damage loss of weight and germination of seedbeans. However, radiation resulted in abnamal seedlings, showing various degrees of morphological malformation, although there was no effect on germination rates or seedling vigor. (Author) [pt

  16. Resistencia a sequía terminal en frijol negro tropical Comportamiento de variedades de maíz normal y con alta calidad de proteína para la región Golfo de México

    Directory of Open Access Journals (Sweden)

    Mark Frahm

    2003-01-01

    Full Text Available Con el objetivo de identificar cultivares de frijol negro tropical con resistencia a sequía y adaptación a la región Centroamericana, se diseñó un proyecto piloto colaborativo entre los programas de frijol de Zamorano, Honduras; Veracruz, México y la Universidad Estatal de Michigan (MSU; EE.UU, en el año 2002. Se desarrollaron dos poblaciones de líneas endog ámicas recombinantes (RILs derivadas de cruces entre la lí- nea resistente a sequía B98311 de MSU con TLP 19 y VAX 5, dos líneas mejoradas del CIAT con resistencia a enfermedades y adaptadas a las condiciones de América Central y México. Ambas poblaciones se evaluaron bajo riego y sequía terminal en Zamorano, Honduras y Veracruz, México. Los rendimientos se vieron reducidos por la sequía y por Macrophomina phaseolina, hongo causal de la pudrición carbonosa. El estrés por sequía, la presión de la enfermedad y los bajos rendimientos, contribuyeron a la obtención de altos coeficientes de variación, lo que hizo difícil la identificación y selecci ón de líneas superiores. La selección se basó en la posici ón de la media geométrica del rendimiento (MG calculada para cada línea con los datos de ambos tratamientos, riego y sequía. La línea L88-63 ocupó el primer lugar en la MG del rendimiento en ambas localidades. Evaluaciones posteriores conducidas en Honduras y Michigan, confirmaron el alto potencial de rendimiento y amplia adaptación de la línea L88- 63. El mejoramiento de la resistencia a la sequía en ambientes del trópico bajo, requiere la incorporación adicional de resistencia a M. phaseolina en las nuevas líneas consideradas para liberarse como nuevas variedades en esta región.

  17. Surface Chloride Levels in Colorado Structural Concrete

    Science.gov (United States)

    2018-01-01

    This project focused on the chloride-induced corrosion of reinforcing steel in structural concrete. The primary goal of this project is to analyze the surface chloride concentration level of the concrete bridge decks throughout Colorado. The study in...

  18. BLM Colorado Oil & Gas Geophysical Permits (Dissolved)

    Data.gov (United States)

    Department of the Interior — KMZ File Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  19. BLM Colorado Oil and Gas Leases (Dissolved)

    Data.gov (United States)

    Department of the Interior — KMZ File Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  20. BLM Colorado Oil & Gas Geophysical Permits (Dissolved)

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  1. BLM Colorado Oil and Gas Units (Dissolve)

    Data.gov (United States)

    Department of the Interior — KMZ File Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  2. BLM Colorado Oil and Gas Leases (Dissolved)

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  3. BLM Colorado Oil and Gas Units (Dissolve)

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  4. Enzymatic changes in intact leaves of Phaseolus vulgaris following ozone fumigation

    Energy Technology Data Exchange (ETDEWEB)

    Dass, H C; Weaver, G M

    1972-01-01

    Enzymatic changes in the intact leaves of Phaseolus vulgaris cv. Seaway 65 were studied following ozone fumigation. It was found that peroxidase enzyme increased significantly with the ozone treatment in the first 48 h. Similarly, cellulase enzyme showed significant increase 48 h. following ozone treatment. Lactic dehydrogenase activity was not markedly affected by ozone treatment. Disc electrophoretic studies of peroxidase isoenzymes showed that ozone treatment induced a new band of peroxidase. The role of peroxidase, cellulase and lactic dehydrogenase enzymes is discussed in relation to ozone damage and the bronzing disorder in white beans. 22 references, 1 figure, 1 table.

  5. Genome sequence of Bradyrhizobium sp. LMTR 3, a diazotrophic symbiont of Lima bean (Phaseolus lunatus

    Directory of Open Access Journals (Sweden)

    Ernesto Ormeño-Orrillo

    2017-09-01

    Full Text Available Bradyrhizobium sp. LMTR 3 is a representative strain of one of the geno(species of diazotrophic symbionts associated with Lima bean (Phaseolus lunatus in Peru. Its 7.83 Mb genome was sequenced using the Illumina technology and found to encode a complete set of genes required for nodulation and nitrogen fixation, and additional genes putatively involved in root colonization. Its draft genome sequence and annotation have been deposited at GenBank under the accession number MAXC00000000.

  6. Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro Análise de restrição do DNA cloroplástico de Phaseolus vulgaris vr. Rio Negro

    Directory of Open Access Journals (Sweden)

    Sergio Echeverrigaray

    1996-12-01

    Full Text Available The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb.O DNA cloroplástico do cultivar Rio Negro (Phaseolus vulgaris L. foi isolado a partir de cloroplastos obtidos por gradiente descontínuo de sacarose. A análise de restrição com as enzimas HindIII, EcoRI e BamHI e a combinação destas, permitiu a identificação de mais de 20 fragmentos na faixa de 18 a 0.65kb. O tamanho do cp DNA de Phaseolus vulgaris L. foi estimado em 140kb com a existência de sequências repetidas de aproximadamente 22kb.

  7. Colorado School Finance Partnership: Report and Recommendations. Financing Colorado's Future: Assessing Our School Finance System

    Science.gov (United States)

    Colorado Children's Campaign, 2012

    2012-01-01

    Over the last decade, Colorado has emerged as a national leader in crafting innovative solutions for challenges facing its public school system. From implementing the Colorado Student Assessment Program (CSAP) and No Child Left Behind (NCLB) reforms to more recent legislation including standards and assessments for a preschool-through-college…

  8. 77 FR 23498 - Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs, CO

    Science.gov (United States)

    2012-04-19

    ... Taylor Museum and the Colorado Springs Fine Arts Center) and the Denver Museum of Nature & Science... Davis, Chief of Staff, President's Office, Colorado College, Armstrong Hall, Room 201, 14 E. Cache La... objects, as well as other cultural items were removed from Canyon de Chelly, Apache County, AZ, under the...

  9. Phenotypic diversity for seed mineral concentration in North American dry bean (Phaseolus vulgaris L.) germplasm of Middle American ancestry

    Science.gov (United States)

    Dry bean (Phaseolus vulgaris L.) seeds are a major protein, carbohydrate, and mineral source for human diets in multiple regions of the world. Seed mineral biofortification is an going objective to improve this important food source. The objective of this research was to assess the seed mineral co...

  10. A non-destructive selection method for faster growth at suboptimal temperature in common bean (Phaseolus vulgaris)

    NARCIS (Netherlands)

    Drijfhout, E.; Oeveren, J.C. van; Jansen, R.C.

    1991-01-01

    A non-destructive method has been developed to select common bean (Phaseolus vulgaris L.) plants whose growth is less effected at a suboptimal temperature. Shoot weight was determined at a suboptimal (14°C) and optimal temperature (20°C), 38 days after sowing and accessions identified with a

  11. Comparison of growth, nitrogen metabolism and organ weights in piglets and rats fed on diets containing Phaseolus vulgaris beans

    NARCIS (Netherlands)

    Huisman, J.; Poel, A.F.B. van der; Leeuwen, P. van; Verstegen, M.W.A.

    1990-01-01

    The effects of lectins in the diet have been mainly studied in rats. An important question is whether results obtained in rats can be extrapolated to larger animals like the pig. Phaseolus vulgaris beans are rich in toxic lectins. Therefore a study was carried out to compare the effects of diets

  12. The interaction between endopolygalacturonase from Fusarium moniliforme and PGIP from Phaseolus Vulgaris studied by surface plasmon resonance and mass spectrometry

    DEFF Research Database (Denmark)

    Mattei, B; Cervone, F; Roepstorff, Peter

    2001-01-01

    Phaseolus vulgaris. PG hydrolyses the homogalacturonan of the plant cell wall and is considered an important pathogenicity factor of many fungi. PGIP is a specific inhibitor of fungal PGs and is thought to be involved in plant defence against phytopathogenic fungi. SPR was used either to study the effect...

  13. Effect of bacterial distribution and activity on conjugal transfer on the phylloplane of the bush bean (Phaseolus vulgaris)

    DEFF Research Database (Denmark)

    Normander, Bo; Christensen, Bjarke Bak; Molin, Søren

    1998-01-01

    Conjugal plasmid transfer was examined on the phylloplane of bean (Phaseolus vulgaris) and related to the spatial distribution pattern and metabolic activity of the bacteria. The donor (Pseudomonas putida KT2442) harbored a derivative of the TOL plasmid, which conferred kanamycin resistance and had...

  14. Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris Enzimas do metabolismo da sacarose em cultura celular de Bauhinia forficata, Curcuma zedoaria e Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Marcia Ometto de Mello

    2001-09-01

    Full Text Available The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.O objetivo deste trabalho foi estudar as enzimas do metabolismo da sacarose em culturas de célula em suspensão de Bauhinia forficata Link, Curcuma zedoaria Roscoe e Phaseolus vulgaris L. A via da invertase foi identificada nas três espécies estudadas. A via da sacarose sintase também foi responsável pelo metabolismo da sacarose em células de Curcuma zedoaria e Phaseolus vulgaris. Foram encontradas atividades maiores que 300 nmol min-1 mg-1 de proteína das enzimas invertase ácida e alcalina, UDPglicose pirofosforilase e fosfoglicomutase no extrato celular das três espécies de plantas. A sacarose sintase mostrou atividade baixa nas células de Bauhinia forficata. À medida que a concentração de sacarose no meio de cultura diminuiu, a atividade da sacarose sintase aumentou em células de Curcuma zedoaria e Phaseolus vulgaris. Ao final do período de cultura, quando os carboidratos se tornaram limitantes, as atividades das enzimas glicolíticas reduziram-se gradualmente.

  15. Dynamics of a Novel Highly Repetitive CACTA Family in Common Bean (Phaseolus vulgaris

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    Dongying Gao

    2016-07-01

    Full Text Available Transposons are ubiquitous genomic components that play pivotal roles in plant gene and genome evolution. We analyzed two genome sequences of common bean (Phaseolus vulgaris and identified a new CACTA transposon family named pvCACTA1. The family is extremely abundant, as more than 12,000 pvCACTA1 elements were found. To our knowledge, this is the most abundant CACTA family reported thus far. The computational and fluorescence in situ hybridization (FISH analyses indicated that the pvCACTA1 elements were concentrated in terminal regions of chromosomes and frequently generated AT-rich 3 bp target site duplications (TSD, WWW, W is A or T. Comparative analysis of the common bean genomes from two domesticated genetic pools revealed that new insertions or excisions of pvCACTA1 elements occurred after the divergence of the two common beans, and some of the polymorphic elements likely resulted in variation in gene sequences. pvCACTA1 elements were detected in related species but not outside the Phaseolus genus. We calculated the molecular evolutionary rate of pvCACTA1 transposons using orthologous elements that indicated that most transposition events likely occurred before the divergence of the two gene pools. These results reveal unique features and evolution of this new transposon family in the common bean genome.

  16. Chemical and biological studies on sweet biscuits produced from irradiated phaseolus beans flour

    International Nuclear Information System (INIS)

    Nassef, A.E.

    2005-01-01

    This study was carried out to evaluate the chemical composition of beans such as minerals, amino acids, total carbohydrates and fiber to produce high quality sweet biscuits for treating some special diseases. In this study, the Phaseolus beans flour was used as a new source of very important composition. Beans flour was irradiated at two doses (0.5 and 1.0 KGy) for preservation. Sweet biscuits were made with supplementation of 5, 10, 15% beans flour. All samples of sweet biscuits were examined for chemical composition and organoleptic characteristics. Biological assay was carried out in rats maintained on 15% either irradiated or non-irradiated beans flour sweet biscuits through determining the weight gain, serum cholesterol and triglycerides and investigating the internal organs. The results obtained showed that sweet biscuits containing 15% Phaseolus beans flour had highest content of protein, minerals and fiber and scored a good grade. Weight gain, cholesterol and triglycerides levels were reduced comparable to control and there was no effect of irradiated beans flour on the internal organs

  17. Phaseolus vulgaris Leuco-Agglutinin Tracing of Intrahypothalamic Connections of the Lateral, Ventromedial, Dorsomedial and Paraventricular Hypothalamic Nuclei in the Rat

    NARCIS (Netherlands)

    Horst, G.J. ter; Luiten, P.G.M.

    Intrahypothalamic connections of the lateral (LHA), ventromedial (VMH), dorsomedial (DMH) and paraventricular (PVN) hypothalamic nuclei were studied with anterograde transport of iontophoretically injected Phaseolus vulgaris leuco-agglutinin and the immunocytochemical detection of labeled

  18. Patterns of Direct Projections from the Hippocampus to the Medial Septum-Diagonal Band Complex : Anterograde Tracing with Phaseolus vulgaris Leucoagglutinin Combined with Immunohistochemistry of Choline Acetyltransferase

    NARCIS (Netherlands)

    Gaykema, R.P.A.; Kuil, J. van der; Hersh, L.B.; Luiten, P.G.M.

    1991-01-01

    The projections from the Ammon's horn to the cholinergic cell groups in the medial septal and diagonal band nuclei were investigated with anterograde tracing of Phaseolus vulgaris leucoagglutinin combined with immunocytochemical detection of choline acetyltransferase, in the rat. Tracer injections

  19. Sauvetage d'embryons chez les légumineuses alimentaires en général et dans le genre Phaseolus en particulier (synthèse bibliographique

    Directory of Open Access Journals (Sweden)

    Barikissou, E.

    2012-01-01

    Full Text Available A review of embryos rescue in food legumes in general and in the genus Phaseolus in particular. Genetic improvement of Phaseolus vulgaris L. by interspecific hybridization with Phaseolus coccineus L. and Phaseolus polyanthus Greenm., used as female parents, often gives rise to embryo abortion at globular developmental stage. In vitro culture of embryos at cotyledonary and torpedo shaped stages, leads to hybrid plants, but with very low percentages of success. Several investigations of in vitro culture in selfed genotypes of Phaseolus and from embryos at globular or young heart shaped stages have allowed to regenerate some young plantlets. However, problems of rooting and stopping of growth restrict the number of developing plantlets. Analysis of the results achieved from interspecific embryo rescue in others food legumes of the genus Lupinus, Cajanus, Cicer, Lens and Trifolium, helped to identify some solutions to resolve incompatibility problems in Phaseolus.

  20. Alternative Fuels Data Center: Colorado Airport Relies on Natural Gas

    Science.gov (United States)

    Fueling Stations Colorado Airport Relies on Natural Gas Fueling Stations to someone by E-mail Share Alternative Fuels Data Center: Colorado Airport Relies on Natural Gas Fueling Stations on Facebook Tweet about Alternative Fuels Data Center: Colorado Airport Relies on Natural Gas Fueling Stations on

  1. BOOKLET TO INSTITUTO PEDAGOGICO NACIONAL TEACHERS ABOUT SYMBIOSIS AND PROCESSES ON BIOTECHNOLOGY: THE BIOFERTILIZER Rhizobium sp IN Phaseolus vulgaris WITH ALTERNATIVE TO SYMBIOSIS FOR Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    María Camila Quevedo Rubiano

    2016-09-01

    Full Text Available This article presents the results of the thesis carried out in the research group of Biotechnology Teaching in Colombia, with the aim of providing teachers of Biology of Instituto Pedagogico Nacional a booklet that can strengthen the teaching of biotechnology processes using Rhizobium sp reduction of chemical fertilizers and symbiosis with Phaseolus vulgaris.   The booklet contains a proposal of practical activities that enable teachers of this institution to use spaces like the farm, enabling to teach biotechnology related to agronomy. Therefore, for this project was considered two Biological and Pedagogical approaches, the first is within the analytical empirical paradigm in the process of microbiological characterization of Rhizobium and their Biofertilizing ability in beans; and the teaching approach within the design of a booklet that includes the findings of this study as a contribution to the reduction of chemical fertilizers school farm. In order to have a complete analysis of the work it was subjected to quantitative and qualitative methods.   This biotech practice is included in the booklet showing in bioassays that bacteria has biofertilizer without inhibiting potential symbiosis, and that research and teaching biological concepts from scientific expertise can be promoted in Biology class for students to understand its context in a significant way, to be used in different levels of education; also it is a teaching strategy.

  2. Sensitivity of Phaseolus vulgaris cv. `CIAP 7247F' plants to Glufosinate ammonium herbicide in greenhouse

    Directory of Open Access Journals (Sweden)

    Idalmis Bermúdez-Caraballoso

    2014-01-01

    Full Text Available Genetic breeding in Phaseolus by genetic transformation requires an efficient selection system. The present investigation was aimed to determine the minimum lethal concentration of glufosinate-ammonium (Finale ® in beans plants cv. `CIAP 7247F' grown in greenhouse. Different concentrations of the herbicide were applied to the foliage of plants in acclimatization phase (20, 30 y 40 mg l-1 and the control. Results showed that the minimum lethal concentration in plants in acclimatization phase was 30 mg l-1. Results also demonstrated that is possible the use of the herbicide as a selective agent of beans transformants cv. `CIAP 7247F' carrying the bar gene. Keywords: genetic transformation, herbicide, selective agent, tissue culture

  3. Starch and sucrose synthesis in Phaseolus vulgaris as affected by light, CO2, and abscisic acid

    International Nuclear Information System (INIS)

    Sharkey, T.D.; Berry, J.A.; Raschke, K.

    1985-01-01

    Phaseolus vulgaris L. leaves were subjected to various light, CO 2 , and O 2 levels and abscisic acid, then given a 10 minute pulse of 14 CO 2 followed by a 5 minute chase with unlabeled CO 2 . After the chase period, very little label remained in the ionic fractions except at low CO 2 partial pressure. Most label was found in the neutral, alcohol soluble fraction or in the insoluble fraction digestable by amyloglucosidase. Sucrose formation was linearly related to assimilation rate. Starch formation increased linearly with assimilation rate, but did not occur if the assimilation rate was below 4 micromoles per square meter per second. Neither abscisic acid, nor high CO 2 in combination with low O 2 caused significant perturbations of the sucrose/starch formation ratio. These studies indicate that the pathways for starch and sucrose synthesis both are controlled by the rate of net CO 2 assimilation, with sucrose the preferred product at very low assimilation rates

  4. Iron and ferritin accumulate in separate cellular locations in Phaseolus seeds

    DEFF Research Database (Denmark)

    Cvitanich, Cristina; Przybylowicz, Wojciech J; Urbanski, Dorian Fabian

    2010-01-01

    and will assist in the production of staples with increased bioavailable iron. Results Here we reveal the distribution of iron in seeds of three Phaseolus species including thirteen genotypes of P. vulgaris, P. coccineus, and P. lunatus. We showed that high concentrations of iron accumulate in cells surrounding...... the provascular tissue of P. vulgaris and P. coccineus seeds. Using the Perls' Prussian blue method, we were able to detect iron in the cytoplasm of epidermal cells, cells near the epidermis, and cells surrounding the provascular tissue. In contrast, the protein ferritin that has been suggested as the major iron...... to P. vulgaris and P. coccineus, we did not observe iron accumulation in the cells surrounding the provascular tissues of P. lunatus cotyledons. A novel iron-rich genotype, NUA35, with a high concentration of iron both in the seed coat and cotyledons was bred from a cross between an Andean...

  5. Development of 12 Chloroplast Microsatellite Markers in Vigna unguiculata (Fabaceae and Amplification in Phaseolus vulgaris

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    Lei Pan

    2014-03-01

    Full Text Available Premise of the study: Vigna unguiculata is an economically important legume, and the complexity of its variability and evolution needs to be further understood. Based on publicly available databases, we developed chloroplast microsatellite primers to investigate genetic diversity within V. unguiculata and its related species Phaseolus vulgaris. Methods and Results: Twelve polymorphic chloroplast microsatellite markers were developed and characterized in 62 V. unguiculata individuals. The number of alleles per locus varied between two and four, the unbiased haploid diversity per locus ranged from 0.123 to 0.497, and the polymorphism information content varied from 0.114 to 0.369. In cross-species amplifications, nine of these markers showed polymorphism in 29 P. vulgaris individuals. Conclusions: The newly developed chloroplast microsatellite markers exhibit variation in V. unguiculata as well as their transferability in P. vulgaris. These markers can be used to investigate genetic diversity and evolution in V. unguiculata and P. vulgaris.

  6. Fractionation of gamma-emitting fission products absorbed by red kidney beans (Phaseolus vulgaris L.)

    International Nuclear Information System (INIS)

    D'Souza, T.J.; Mistry, K.B.

    1980-01-01

    The gamma-emitting fission product nuclides 106 Ru, 125 Sb, 137 Cs and 144 Ce that accumulated in the edible pods of bean (Phaseolus vulgaris L.) plants grown in nutrient culture were subjected to chemical fractionation. The results indicated that the largest fraction of 106 Ru, 125 Sb and 144 Ce was associated with ionic forms including salts of organic acids, phosphates, carbonates and some protein-bound forms extracted with dilute mineral acids (acid fraction). The association of these radionuclides with lipids including lipophyllic pigments, free amino acids and amino sugars (ethanol fraction) was next in significance. The association of 137 Cs was, however, greater with the ethanol fraction than with the acid fraction. Considerably reduced amounts of the fission products were present in the pectates, proteins, polysaccharides and nucleic acids. (U.K.)

  7. Yield and drougth tolerance of six varieties of common bean (Phaseolus vulgaris L. under field condition

    Directory of Open Access Journals (Sweden)

    Yanitza Meriño Hernandez

    2015-01-01

    Full Text Available In two moisture conditions (drought and irrigation were evaluated six varieties of common bean (Phaseolus vulgaris L., with a factorial randomized complete blocks. The objectives of the study was to evaluate the effect caused by drought conditions crop varieties, identify high performance and features that enable them to adapt to varying conditions of soil moisture. With the data in yields between the two humidity conditions intensity indices of drought (IIS, susceptibility to drought (ISS, relative efficiency (IER, geometric mean (GM and percent yield losses were calculated . The results were statistically processed using the Statistica software version 8.0 for Windows, if significant differences Tukey test was applied to p<0.05. The selection based on levels ISS, MG, IER and PPR identified high yielding varieties adapted to drought and favorable moisture conditions.

  8. Effect of lead on imbibition, germination, and growth of Phaseolus vulgaris L. and Zea mays L.

    Directory of Open Access Journals (Sweden)

    Gustavo Isaza Guzmán Isaza Guzmán

    2013-07-01

    Full Text Available Lead is highly reactive and it can be consequently toxic to living cells to both plants and humans. This heavy metal is a source of contamination to the environment and it disrupts natural cycles. The present study was aimed to evaluate the effect of lead on the imbibition process, germination and growth in the bean (Phaseolus vulgaris L. and maize (Zea mays L.. It was developed a system consisting of receptacles to expose flooded plants at different concentrations of the metal. Results showed that at concentrations of 5 g l-1 lead imbibition process was affected, but was more evident in bean. Germination percentage was not affected in maize seeds, while viability was affected in bean seeds. We observed statistically that there is an effect on organ growth of root, stem and leaf in both species in the presence of solution whose effect is most noticeable in bean plants. Key words: heavy metals,phytoremediation, stress, toxic substances

  9. Phylogenetic relationships and host range of Rhizobium spp. that nodulate Phaseolus vulgaris L.

    Science.gov (United States)

    Hernandez-Lucas, I; Segovia, L; Martinez-Romero, E; Pueppke, S G

    1995-07-01

    We determined the nucleotide sequences of 16S rRNA gene segments from five Rhizobium strains that have been isolated from tropical legume species. All share the capacity to nodulate Phaseolus vulgaris L., the common bean. Phylogenetic analysis confirmed that these strains are of two different chromosomal lineages. We defined the host ranges of two strains of Rhizobium etli and three strains of R. tropici, comparing them with those of the two most divergently related new strains. Twenty-two of the 43 tested legume species were nodulated by three or more of these strains. All seven strains have broad host ranges that include woody species such as Albizia lebbeck, Gliricidia maculata, and Leucaena leucocephala.

  10. Effect of different nitrogen sources on plant characteristics and yield of common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Fernández-Luqueño, F; Reyes-Varela, V; Martínez-Suárez, C; Salomón-Hernández, G; Yáñez-Meneses, J; Ceballos-Ramírez, J M; Dendooven, L

    2010-01-01

    Wastewater sludge can be used to fertilize crops, especially after vermicomposting (composting with earthworms to reduce pathogens). How wastewater sludge or vermicompost affects bean (Phaseolus vulgaris L.) growth is still largely unknown. In this study the effect of different forms of N fertilizer on common bean plant characteristics and yield were investigated in a Typic Fragiudepts (sandy loam) soil under greenhouse conditions. Beans were fertilized with wastewater sludge, or wastewater sludge vermicompost, or urea, or grown in unamended soil, while plant characteristics and yield were monitored (the unamended soil had no fertilization). Yields of common bean plants cultivated in unamended soil or soil amended with urea were lower than those cultivated in wastewater sludge-amended soil. Application of vermicompost further improved plant development and increased yield compared with beans cultivated in wastewater amended soil. It was found that application of organic waste products improved growth and yield of bean plants compared to those amended with inorganic fertilizer.

  11. Pinto Beans (Phaseolus vulgaris L. as a Functional Food: Implications on Human Health

    Directory of Open Access Journals (Sweden)

    Vicki Schlegel

    2013-02-01

    Full Text Available Most foods are considered functional in terms of providing nutrients and energy to sustain daily life, but dietary systems that are capable of preventing or remediating a stressed or diseased state are classified as functional foods. Dry beans (Phaseolus vulgaris L. contain high levels of chemically diverse components (phenols, resistance starch, vitamins, fructooligosaccharides that have shown to protect against such conditions as oxidative stress, cardiovascular disease, diabetes, metabolic syndrome, and many types of cancer, thereby positioning this legume as an excellent functional food. Moreover, the United States has a rich dry bean history and is currently a top producer of dry beans in the world with pinto beans accounting for the vast majority. Despite these attributes, dry bean consumption in the US remains relatively low. Therefore, the objective of this manuscript is to review dry beans as an important US agricultural crop and as functional food for the present age with an emphasis on pinto beans.

  12. Novel vitamin E forms in leaves of Kalanchoe daigremontiana and Phaseolus coccineus.

    Science.gov (United States)

    Kruk, Jerzy; Pisarski, Adam; Szymańska, Renata

    2011-11-15

    In the present study, we isolated novel tocochromanols from green leaves of Kalanchoe daigremontiana and primary leaves of etiolated seedlings of Phaseolus coccineus that were identified as β-, γ-, and δ-tocomonoenols with unsaturation at the terminal isoprene unit of the side chain. The content of γ-tocomonoenol in leaves of etiolated bean increased gradually with the age of seedlings, reaching 50% of the γ-tocopherol level in 40-day-old plants. The content of this compound in leaves was increased by short illumination of etiolated plants and by addition of homogentisic acid, a biosynthetic precursor of tocopherols. These data indicated that γ-tocomonoenol is synthesized de novo from homogentisic acid and tetrahydro-geranylgeraniol diphosphate, a phytol precursor. Based on these results, a biosynthetic pathway of tocomonoenols is proposed. Copyright © 2011 Elsevier GmbH. All rights reserved.

  13. Nutritive value evaluated on rats of new cultivars of common beans (Phaseolus vulgaris) released in Chile.

    Science.gov (United States)

    Yañez, E; Zacarias, I; Aguayo, M; Vasquez, M; Guzman, E

    1995-06-01

    Five new cultivars of common beans (Phaseolus vulgaris) recently released were analyzed for their proximate chemical composition and protein biological quality. The crude protein content in these cultivars ranged from 21.9 percent in cultivar Arroz 3 to 26.9 percent in cultivar Tórtola Diana (dry matter basis). Rats fed cultivar Tórtola INIA gained more weight, had a higher protein intake and registered higher PER and NPR than Tórtola corriente. On the other hand, rats consuming cultivars Arroz 3 and Fleetwood had lower weight gain, lower protein intake and lower PER and NPR than cultivar Coscorrón corriente. However, all these cultivars have a relatively good protein value as compared to other plant protein sources.

  14. Gamma radiation effects on bean plants (Phaseolus vulgaris L.) in flowering

    International Nuclear Information System (INIS)

    Tulmann Neto, A.; Matsumoto, K.; Marchezoni, S.A.; Ando, A.; Menten, J.O.M.

    1980-01-01

    The possibility of utilizing the 60 Co source in the Center of Nuclear Energy for Agriculture (CENA), Sao Paulo University, for gamma-irradiation of plants in flower was shown by an experiment with beans (Phaseolus vulgaris L.). Pots with two bean plants in flower, variety Carioca, line 6E 1 , were put individually in the center of the source. Doses used were 1, 2, 3, 4 and 5 kR. The development of these plants after irradiation till harvest and seedling emergence of their progeny were observed. The effects of gamma-rays and the advantages of irradiation of plants in flower were discussed, and recommendable procedures for research workers who need to use the 60 Co source of the CENA are suggested. (Author) [pt

  15. Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua.

    Science.gov (United States)

    Marcenaro, Delfia; Valkonen, Jari P T

    2016-01-01

    Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, 'INTA Rojo'. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar 'INTA Rojo' and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology

  16. Aplicação de óleo no controle de Zabrotes subfasciatus e na germinação de Phaseolus vulgaris Oil aplication in the control of Zabrotes subfasciatus and in the germination of Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Maiene de F. C. de Queiroga

    2012-07-01

    Full Text Available Propôs-se, com este trabalho, estudar a qualidade de sementes de feijão carioca (Phaseolus vulgaris L. tratadas com óleos vegetais de mamona, soja e oiticica, durante cinco meses de armazenamento. Mediante os resultados obtidos concluiu-se que os óleos vegetais utilizados no tratamento das sementes de feijão Phaseolus foram eficientes na manutenção da viabilidade e no controle da infestação pelo inseto-praga de armazenamento Zabrotes subfasciatus, nos cinco meses de armazenamento, sendo o óleo de oiticica o que apresentou melhor média de germinação, frente às tratadas com óleo de mamona e soja. O óleo de oiticica também foi o mais eficiente no controle de Z. subfasciatus. Verificou-se, ainda, redução da eficiência dos óleos nas suas menores doses, sendo a dose de 4,5 mL para 500 g de sementes, a mais eficaz para todas as variáveis estudadas.The purpose of this work was to study the quality of bean seeds (Phaseolus vulgaris L. treated with vegetable oils of castor, soybean and 'oiticica' during five months of storage. From the obtained results it was concluded that vegetable oils used in the treatment of seeds of Phaseolus beans were effective in maintaining the viability and control of storage insect pest infestation Zabrotes subfasciatus during the five months of storage, and the 'oiticica' oil presented the best mean germination, compared to those treated with castor oil and soybeans. 'Oiticica' oil was also the most efficient in controlling Z. subfasciatus. Reduced efficiency of the oils was observed in the small doses and the dose of 4.5 mL for 500 g of seeds was the most effective for all variables.

  17. The Chapter 1 Challenge: Colorado's Contribution 1993.

    Science.gov (United States)

    Petro, Janice Rose; And Others

    An overview is provided of Colorado's participation in Chapter 1, the largest federally funded program designed to provide services to elementary and secondary students. Chapter 1 provides financial assistance to state and local education agencies to meet the special needs of educationally deprived children who reside in areas with high…

  18. Insects associated with ponderosa pine in Colorado

    Science.gov (United States)

    Robert E. Stevens; J. Wayne Brewer; David A. Leatherman

    1980-01-01

    Ponderosa pine serves as a host for a wide variety of insects. Many of these, including all the particularly destructive ones in Colorado, are discussed in this report. Included are a key to the major insect groups, an annotated list of the major groups, a glossary, and a list of references.

  19. Bats of the Colorado oil shale region

    Energy Technology Data Exchange (ETDEWEB)

    Finley, R.B. Jr.; Caire, W.; Wilhelm, D.E.

    1984-10-31

    New records for Myotis californicus, M. evotis, M. leibii, M. lucifugus, M. thysanodes, M. volans, M. yumanensis, Lasionycteris noctivagans, Pipistrellus hesperus, Eptesicus fuscus, Lasiurus cinereus, Plecotus townsendii, and Antrozous pallidus and their habitat occurrence in northwestern Colorado are reported. Mortality of 27 bats of six species trapped in an oil sludge pit is described. 7 references.

  20. 76 FR 76109 - Colorado Regulatory Program

    Science.gov (United States)

    2011-12-06

    ... its program to improve operational efficiency. This document gives the times and locations that the... the amendment during regular business hours at the following locations: Kenneth Walker, Chief, Denver... available for you to read at the locations listed above under ADDRESSES. Specifically, Colorado proposes...

  1. Feeding stimulants for the colorado beetle

    NARCIS (Netherlands)

    Ritter, F.J.

    1967-01-01

    Potato leaf extract was fractionated and the fractions obtained were tested for their activity as feeding stimulants for Colorado beetle larvae. Also leaves and leaf extracts of different kinds of plants, as well as a number of known pure compounds and mixtures of them, were tested for this

  2. Effects of Fertilization on Uptake of 85Sr, 60Co and 54Mn by Tomato and Phaseolus Plants

    International Nuclear Information System (INIS)

    Ramadan, A.B.; Ezz El-Din, M.R.

    2001-01-01

    The effects of N-, P-, and K- fertilizers on availability of 85 Sr, 60 Co and 54 Mn added to the soil were measured in an open field experiment. The uptake of 85 Sr, 60 Co and 54 Mn by tomato and phaseolus was lower in fertilized treatments than in unfertilized ones. The radionuclide availability under fertilized condition depends on soil and element properties. Solubilization of Ca-ions following nitrification of nitrogen in ammonium salts and the presence of stable Strontium, Cobalt and Manganese in the acidifying fertilizers are the main factors giving rise to the reduced radioisotopes uptake by plants. The relative order of uptake of the investigated radionuclides by plants appeared to be as follow 54 Mn> 60 Co> 85 Sr. The distribution pattern of the total absorbed radionuclides in the two plants shows that the shoots contained the highest percent of these radionuclides. Transfer factors for phaseolus plants were higher than those of tomato plants

  3. The seed coat of Phaseolus vulgaris interferes with the development of the cowpea weevil [Callosobruchus maculatus (F. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Silva Luciana B.

    2004-01-01

    Full Text Available We have confirmed here that the seeds of the common bean (Phaseolus vulgaris, L. do not support development of the bruchid Callosobruchus maculatus (F., a pest of cowpea [Vigna unguiculata (L. Walp] seeds. Analysis of the testa (seed coat of the bean suggested that neither thickness nor the levels of compounds such as tannic acid, tannins, or HCN are important for the resistance. On the other hand, we have found that phaseolin (vicilin-like 7S storage globulin, detected in the testa by Western blotting and N-terminal amino acid sequencing, is detrimental to the development of C. maculatus. As for the case of other previously studied legume seeds (Canavalia ensiformis and Phaseolus lunatus we suggest that the presence of vicilin-like proteins in the testa of P. vulgaris may have had a significant role in the evolutionary adaptation of bruchids to the seeds of leguminous plants.

  4. Understanding uncertainties in future Colorado River streamflow

    Science.gov (United States)

    Julie A. Vano,; Bradley Udall,; Cayan, Daniel; Jonathan T Overpeck,; Brekke, Levi D.; Das, Tapash; Hartmann, Holly C.; Hidalgo, Hugo G.; Hoerling, Martin P; McCabe, Gregory J.; Morino, Kiyomi; Webb, Robert S.; Werner, Kevin; Lettenmaier, Dennis P.

    2014-01-01

    The Colorado River is the primary water source for more than 30 million people in the United States and Mexico. Recent studies that project streamf low changes in the Colorado River all project annual declines, but the magnitude of the projected decreases range from less than 10% to 45% by the mid-twenty-first century. To understand these differences, we address the questions the management community has raised: Why is there such a wide range of projections of impacts of future climate change on Colorado River streamflow, and how should this uncertainty be interpreted? We identify four major sources of disparities among studies that arise from both methodological and model differences. In order of importance, these are differences in 1) the global climate models (GCMs) and emission scenarios used; 2) the ability of land surface and atmospheric models to simulate properly the high-elevation runoff source areas; 3) the sensitivities of land surface hydrology models to precipitation and temperature changes; and 4) the methods used to statistically downscale GCM scenarios. In accounting for these differences, there is substantial evidence across studies that future Colorado River streamflow will be reduced under the current trajectories of anthropogenic greenhouse gas emissions because of a combination of strong temperature-induced runoff curtailment and reduced annual precipitation. Reconstructions of preinstrumental streamflows provide additional insights; the greatest risk to Colorado River streamf lows is a multidecadal drought, like that observed in paleoreconstructions, exacerbated by a steady reduction in flows due to climate change. This could result in decades of sustained streamflows much lower than have been observed in the ~100 years of instrumental record.

  5. Local (gut) and systemic metabolism of rats is altered by consumption of raw bean (Phaseolus vulgaris L var athropurpurea).

    Science.gov (United States)

    Santidrián, Santiago; de Moya, Carmen Cavallé; Grant, George; Frühbeck, Gema; Urdaneta, Elena; García, María; Marzo, Florencio

    2003-03-01

    The composition of the raw legume Phaseolus vulgaris L. var. athropurpurea (PhVa) and its effects on the metabolism of young growing rats have been evaluated. The levels of protein, unsaturated fatty acids, carbohydrate, fibre and bioactive factors present in PhVa were comparable with those in other Phaseolus vulgaris varieties. However, the lectins of PhVa were predominantly of the leucoagglutinating type, and concentrated in the albumin protein fraction. Rats fed a diet (110 g total protein, 16.0 MJ/kg) in which PhVa meal provided about half of the protein excreted high levels of N in faeces and urine, and grew more slowly, than rats fed a high-quality control diet (ad libitum or pair-fed). Small intestine, large intestine and pancreas weights were increased (by almost 100 %, PPhVa, which were concentrated in the albumin protein fraction, whereas in many other Phaseolus vulgaris lines they are distributed across the globulin and albumin fractions.

  6. Fluorescence In Situ Hybridization (FISH-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris and Relatives

    Directory of Open Access Journals (Sweden)

    Aiko Iwata-Otsubo

    2016-04-01

    Full Text Available Fluorescence in situ hybridization (FISH-based karyotyping is a powerful cytogenetics tool to study chromosome organization, behavior, and chromosome evolution. Here, we developed a FISH-based karyotyping system using a probe mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and chromosome-specific BAC clones in common bean, which enables one to unambiguously distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping system to several wild relatives and landraces of common bean from two distinct gene pools, as well as other related Phaseolus species, to investigate repeat evolution in the genus Phaseolus. Comparison of karyotype maps within common bean indicates that chromosomal distribution of the centromeric and subtelomeric satellite repeats is stable, whereas the copy number of the repeats was variable, indicating rapid amplification/reduction of the repeats in specific genomic regions. In Phaseolus species that diverged approximately 2–4 million yr ago, copy numbers of centromeric repeats were largely reduced or diverged, and chromosomal distributions have changed, suggesting rapid evolution of centromeric repeats. We also detected variation in the distribution pattern of subtelomeric repeats in Phaseolus species. The FISH-based karyotyping system revealed that satellite repeats are actively and rapidly evolving, forming genomic features unique to individual common bean accessions and Phaseolus species.

  7. Gunnison, Colorado subpile study report. Revision 1

    International Nuclear Information System (INIS)

    1994-08-01

    To protect human health and the environment, the Uranium Mill Tailings Remedial Action (UMTRA) Project will remediate the uranium mill tailings site at Gunnison Colorado. There are explicit requirements (i.e., 40 CFR Part 192) for the surface remediation of radiologically contaminated soils on UMTRA sites. The removal of subpile sediment to the depth required by 40 CFR Part 192 will leave in place deeper foundation sediment that is contaminated with hazardous constituents other than radium-226 and thorium-230. The Department of Energy and the Colorado Department of Health have questioned whether this contaminated soil could potentially act as a continuing source of ground water contamination even after surface remediation based on 40 CFR Part 192 is complete. To evaluate the subpile sediments as a potential source of ground water contamination, the Gunnison Subpile study was initiated. This report summarizes the results and findings of this study

  8. Mineral exploration with ERTS imagery. [Colorado

    Science.gov (United States)

    Nicolais, S. M.

    1974-01-01

    Ten potential target areas for metallic mineral exploration were selected on the basis of a photo-lineament interpretation of the ERTS image 1172-17141 in central Colorado. An evaluation of bias indicated that prior geologic knowledge of the region had little, if any, effect on target selection. In addition, a contoured plot of the frequency of photo-lineament intersections was made to determine what relationships exist between the photo-lineaments and mineral districts. Comparison of this plot with a plot of the mineral districts indicates that areas with a high frequency of intersections commonly coincide with known mineral districts. The results of this experiment suggest that photo-lineaments are fractures or fracture-controlled features, and their distribution may be a guide to metallic mineral deposits in Colorado, and probably other areas as well.

  9. Identification and analysis of common bean (Phaseolus vulgaris L. transcriptomes by massively parallel pyrosequencing

    Directory of Open Access Journals (Sweden)

    Thimmapuram Jyothi

    2011-10-01

    Full Text Available Abstract Background Common bean (Phaseolus vulgaris is the most important food legume in the world. Although this crop is very important to both the developed and developing world as a means of dietary protein supply, resources available in common bean are limited. Global transcriptome analysis is important to better understand gene expression, genetic variation, and gene structure annotation in addition to other important features. However, the number and description of common bean sequences are very limited, which greatly inhibits genome and transcriptome research. Here we used 454 pyrosequencing to obtain a substantial transcriptome dataset for common bean. Results We obtained 1,692,972 reads with an average read length of 207 nucleotides (nt. These reads were assembled into 59,295 unigenes including 39,572 contigs and 19,723 singletons, in addition to 35,328 singletons less than 100 bp. Comparing the unigenes to common bean ESTs deposited in GenBank, we found that 53.40% or 31,664 of these unigenes had no matches to this dataset and can be considered as new common bean transcripts. Functional annotation of the unigenes carried out by Gene Ontology assignments from hits to Arabidopsis and soybean indicated coverage of a broad range of GO categories. The common bean unigenes were also compared to the bean bacterial artificial chromosome (BAC end sequences, and a total of 21% of the unigenes (12,724 including 9,199 contigs and 3,256 singletons match to the 8,823 BAC-end sequences. In addition, a large number of simple sequence repeats (SSRs and transcription factors were also identified in this study. Conclusions This work provides the first large scale identification of the common bean transcriptome derived by 454 pyrosequencing. This research has resulted in a 150% increase in the number of Phaseolus vulgaris ESTs. The dataset obtained through this analysis will provide a platform for functional genomics in common bean and related legumes and

  10. Green pricing: A Colorado case study

    Energy Technology Data Exchange (ETDEWEB)

    Blank, E.; Udall, J.R.

    1996-12-31

    A model program for green pricing targeted primarily at large customers is proposed in this paper. The program would create a partnership between a local community group, a renewables advocacy group, and several Colorado utilities. The first part of the paper summarizes pertinent background issues, including utility experience with green pricing programs. The rest of the paper outlines the program proposal, focusing primarily on organizational structure.

  11. Colorado family physicians' attitudes toward medical marijuana.

    Science.gov (United States)

    Kondrad, Elin; Reid, Alfred

    2013-01-01

    Over the last decade, the use of medical marijuana has expanded dramatically; it is now permitted in 16 states and the District of Columbia. Our study of family physicians in Colorado is the first to gather information about physician attitudes toward this evolving practice. We distributed an anonymous web-based electronic survey to the 1727 members of the Colorado Academy of Family Physicians' listserv. Items included individual and practice characteristics as well as experience with and attitudes toward medical marijuana. Five hundred twenty family physicians responded (30% response rate). Of these, 46% did not support physicians recommending medical marijuana; only 19% thought that physicians should recommend it. A minority thought that marijuana conferred significant benefits to physical (27%) and mental (15%) health. Most agreed that marijuana poses serious mental (64%) and physical (61%) health risks. Eighty-one percent agreed that physicians should have formal training before recommending medical marijuana, and 92% agreed that continuing medical education about medical marijuana should be available to family physicians. Despite a high prevalence of use in Colorado, most family physicians are not convinced of marijuana's health benefits and believe its use carries risks. Nearly all agreed on the need for further medical education about medical marijuana.

  12. Economic impact study of the Uranium Mill Tailings Remedial Action project in Colorado: Colorado state fiscal year 1995

    International Nuclear Information System (INIS)

    1995-12-01

    This Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year (FY) 1995 (1 July 1994 through 30 June 1995). To capture employment information, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Gunnison, Maybell, Naturita, Rifle, and Slick Rock, Colorado. Economic data were requested from the Remedial Action Contractor (RAC), the Technical Assistance Contractor (TAC) and the US Department of Energy (DOE). The most significant benefits associated with the UMTRA Project in Colorado are summarized

  13. Economic impact study of the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado state fiscal year 1993

    International Nuclear Information System (INIS)

    1993-12-01

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year 1993 (July 1, 1992, through June 30, 1993). To capture employment benefits, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Rifle, and Gunnison, Colorado. An estimated 52 percent of the employees working on the UMTRA Project responded to this information request. Economic data were requested from each site prime subcontractor, as well as from the Remedial Action Contractor. The most significant benefits associated with the UMTRA Project in Colorado are summarized

  14. Genetic control of common bean (Phaseolus vulgaris resistance to powdery mildew (Erysiphe polygoni

    Directory of Open Access Journals (Sweden)

    Rezende Viviane Ferreira

    1999-01-01

    Full Text Available Genetic control of common bean (Phaseolus vulgaris resistance to powdery mildew (Erysiphe polygoni was studied using segregating populations from the bean variety crosses Jalo x ESAL 686 and ESAL 550 x ESAL 686. F2 plants, together with the parents, were inoculated and evaluated using a scale of values from one (plant without symptoms to nine (completely infected plant. F2 plants were harvested individually, and F2:3 families were obtained. These families were evaluated in an 11 x 11 and 12 x 12 simple lattice statistical design for the Jalo x ESAL 686 and ESAL 550 x ESAL 686 crosses, respectively, using the same value scale as the F2 generation. The segregation observed in F2 plants and F2:3 families indicated that two genes are involved in genetic control, due to a double recessive epistasis. The high linear regression coefficient (b between F2 plants and their F2:3 family, 0.66 for ESAL 550 x ESAL 686 cross, and 0.71 for Jalo x ESAL 686 cross, showed that the trait is highly heritable.

  15. Interaction of cold radiofrequency plasma with seeds of beans (Phaseolus vulgaris)

    Science.gov (United States)

    Bormashenko, Edward; Shapira, Yekaterina; Grynyov, Roman; Whyman, Gene; Bormashenko, Yelena; Drori, Elyashiv

    2015-01-01

    The impact of cold radiofrequency air plasma on the wetting properties and water imbibition of beans (Phaseolus vulgaris) was studied. The influence of plasma on wetting of a cotyledon and seed coat (testa) was elucidated. It was established that cold plasma treatment leads to hydrophilization of the cotyledon and tissues constituting the testa when they are separately exposed to plasma. By contrast, when the entire bean is exposed to plasma treatment, only the external surface of the bean is hydrophilized by the cold plasma. Water imbibition by plasma-treated beans was studied. Plasma treatment markedly accelerates the water absorption. The crucial role of a micropyle in the process of water imbibition was established. It was established that the final percentage of germination was almost the same in the cases of plasma-treated, untreated, and vacuum-pumped samples. However, the speed of germination was markedly higher for the plasma-treated samples. The influence of the vacuum pumping involved in the cold plasma treatment on the germination was also clarified. PMID:25948708

  16. Polyphenol-Rich Dry Common Beans (Phaseolus vulgaris L.) and Their Health Benefits

    Science.gov (United States)

    Ganesan, Kumar

    2017-01-01

    Polyphenols are plant metabolites with potent anti-oxidant properties, which help to reduce the effects of oxidative stress-induced dreaded diseases. The evidence demonstrated that dietary polyphenols are of emerging increasing scientific interest due to their role in the prevention of degenerative diseases in humans. Possible health beneficial effects of polyphenols are based on the human consumption and their bioavailability. Common beans (Phaseolus vulgaris L.) are a greater source of polyphenolic compounds with numerous health promoting properties. Polyphenol-rich dry common beans have potential effects on human health, and possess anti-oxidant, anti-diabetic, anti-obesity, anti-inflammatory and anti-mutagenic and anti-carcinogenic properties. Based on the studies, the current comprehensive review aims to provide up-to-date information on the nutritional compositions and health-promoting effect of polyphenol-rich common beans, which help to explore their therapeutic values for future clinical studies. Investigation of common beans and their impacts on human health were obtained from various library databases and electronic searches (Science Direct PubMed, and Google Scholar). PMID:29113066

  17. Incorporation of 14CO2 by illuminated intact leaves of bean (PHASEOLUS VULGARIS) plants

    International Nuclear Information System (INIS)

    Andrade, A.G. de

    1980-01-01

    Bean plants were grown in hydroponic nutrient solution, maintained in controlled environment. Measurements of the photosynthetic activity using the method of 14 CO 2 incorporation in intact leaves with portable equipment were made on the central leaflet of the first trifoliate leaf except when the effect of leaf age was studied in which case all central leaflets of the same branch were used. The data obtained indicated differences in the photosynthetic efficiency of bean (Phaseolus vulgaris) cultivars. Relative differences in RuDP carboxylase activity in the crude extracts of leaves, leaf area and leaf chlorophyll content were also observed. Rates of 14 CO 2 incorporation at saturating light varied from 14.94 to 22.96 mg CO 2 .dm -2 .h and the 6 studied cultivars could be divided into two classes: Classe 1 (above 20 mg CO 2 .dm -2 .h): Pirata-1, Rosinha G-2, and Pintadinho Precoce; Classe 2 (below 20 mg CO 2 .dm - 2 .h): Carioca, Rosinha Precoce and Pintado. Plants of the same cultivar showed a relatively high variability and a strong dependence in relation to environmental conditions. Differences among cultivars in relation to RuDP carboxylase activity, leaf area and leaf age were correlated to photosynthetic rate. (Author) [pt

  18. CINÉTICA DE FEIJÃO PRETO (PHASEOLUS VULGARIS, L. EM SECADOR DE BANDEJA

    Directory of Open Access Journals (Sweden)

    Deyzi Santos Gouveia

    2011-03-01

    Full Text Available O presente trabalho propôs estudar a cinética de feijão preto (Phaseolus vulgaris, l. em secador de bandeja a diferentes temperaturas do ar e posterior ajuste dos dados experimentais, obtidos com os diferentes modelos matemáticos ( Fick, Page e Cavalcante Mata. O teor de umidade inicial das sementes foi determinado pelo método padrão da estufa, 105 ± 3 °C, durante 72 h, com três repetições, de acordo com as regras do Instituto Adolfo Lutz (1985. Os tratamentos de secagem foram realizados em cinco níveis de temperaturas (40, 50, 60 ,70 e 80 ºC. Para cada tratamento de secagem foram utilizados em torno de 60 g de feijão por repetição. Para este fim utilizou-se um desidratador de frutas, Polidryer PD-25. O ar foi aquecido por meio de gás de cozinha, enquanto a temperatura foi controlada com o auxílio de um termopar, conectado ao secador. Os resultados mostraram que as equações propostas por Cavalcanti Mata e Page foram as que melhor representaram os dados experimentais, quando comparada com a equação de Fick utilizando um termo da série.

  19. Plantas invasoras da cultura do feijoeiro (Phaseolus vulgaris L. no Estado de Minas Gerais

    Directory of Open Access Journals (Sweden)

    Julio Pedro Laca-Buendia

    1989-01-01

    Full Text Available Nas áreas de cultura do feijoeiro (Phaseolus vulgaris L., no Estado de Minas Gerais, foram coletadas e identificadas 222 espécies de plantas invasoras (= plantas daninhas, pertencentes a 35 famílias botânias, representando 118 gêneros, sendo que as famílias Compositae, Leguminosae, Gramineae, Malvaceae, Convolvulaceae, Rubiaceae, Euphorbiaceae, Amaranthaceae, Cyperaceae e Solanaceae, são as mais importantes em relação à cultura. As plantas coletadas, devidamente etiquetadas e identificadas, foram anexadas no PAMG (Herbário da Empresa de Pesquisa Agropecuária de Minas Gerais, Belo Horizonte - (MG..A survey in the cultivation area of bean in the state of Minas Gerais, Brazil, resulted in the determination of 222 weeds species, of 118 genera belonging to 35 families presenting a greater number of species areas: Compositae, Leguminosae, Gramineae, Malvaceae, Convolvulaceae, Rubiaceae, Euphorbiaceae, Amaranthaceae, Cyperaceae and Solanaceae, with 33, 30, 25, 21, 12, 10. 10, 10, 9. 8 species respectively.

  20. Pathogenic variability of Colletotrichum lindemuthianum and Ecuadorian Phaseolus vulgaris L. germplasm resistance

    Directory of Open Access Journals (Sweden)

    Diego Rodríguez-Ortega

    2018-01-01

    Full Text Available Anthracnose caused by Colletotrichum lindemuthianum is one of the most economically important diseases of bean (Phaseolus vulgaris L. cultivation in Ecuador. The best control alternative is the use of resistant varieties. C. lindemuthianum presents great pathogenic variability, which hinders the development of varieties with a lasting resistance, therefore, the knowledge of the presence and distribution of the physiological races of the pathogen and the identification of resistance genes are key to developing varieties with broad and lasting resistance. The objective of this research was to determine the pathogenic variability of C. lindemuthianum and to evaluate the resistance of Ecuadorian bean germplasm. The research was carried out between 2013 and 2014. Seventeen isolates of C. lindemuthianum from northern central Ecuador were characterized by the inoculation of a group of twelve standard differential bean varieties. Among the analyzed samples, thirteen races were identified; five of those races had not been previously reported in the country. The differential G2333 (Co-42, Co-52 and Co-7 presented resistance to every characterized races in Ecuador. In addition, twenty - one improved varieties and elite bean lines were evaluated with sixteen of the seventeen isolates, three genotypes were identified (TB2, TB3 and INIAP 485 Urcuquí with resistance to the mentioned isolates, which can be used as sources of resistance to Anthracnose. The identified sources of resistance in this study will allow to plan the development of bean varieties with broad and durable resistance to C. lindemuthianum.

  1. Study on mutation induced effect of gamma ray and DES on black bean phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Le Thi Dinh; Pham Le Ha; Nguyen Van Toan; Le Xuan Tham [Nuclear Research Institute, Radiobiology Department, Dalat (Viet Nam)

    2001-03-01

    The study on mutation induced effect of gamma ray and DES on black bean Phaseolus vulgaris was carried out at Radiobiology Department, Nuclear Research Institute of Dalat. Dry seeds of variety No.1847 - Bonita - Cuba in set of 13 black bean varieties were irradiated with gamma ray from {sup 60}Co source at dose range from 150 Gy to 350 Gy and treated with DES at concentration from 0.1% to 0.3% in 2 hours for experiments in laboratory. The doses of 200, 250, 300 Gy and concentration of 0.2% DES in 2 hours were selected to treat dry seeds for experiments on the field. In populations of M{sub 1} generation, the height, number of branches and fruits per plant, number of seeds per fruit were decreased with increasing of irradiation doses. In populations of M{sub 2} generation, individual variants in leaf shape, chlorophyll, short stem, dwarf, early maturity, flowering in very short time were obtained and selected in all treatment cases. Mutation frequency at dose of 300 Gy was higher than that in other treatment cases, but ratio of sterility is also largest. The mutant lines of early maturity and short stem with flowering in very short time are promised materials for further studies. (author)

  2. Removal of some metal ions by activated carbon prepared from Phaseolus aureus hulls.

    Science.gov (United States)

    Rao, M Madhava; Ramana, D K; Seshaiah, K; Wang, M C; Chien, S W Chang

    2009-07-30

    Removal of lead [Pb(II)], zinc [Zn(II)], copper [Cu(II)], and cadmium [Cd(II)] from aqueous solutions using activated carbon prepared from Phaseolus aureus hulls (ACPAH), an agricultural waste was studied. The influence of various parameters such as effect of pH, contact time, adsorbent dose, and initial concentration of metal ions on the removal was evaluated by batch method. The removal of metal ions by ACPAH was pH dependent and the optimum pH values were 7.0, 8.0, 7.0 and 6.0 for Cu(II), Cd(II), Zn(II), and Pb(II), respectively. The sorption isotherms were studied using Langmuir, Freundlich, Dubinin-Radushkevich (D-R), and Temkin isotherm models. The maximum adsorption capacity values of ACPAH for metal ions were 21.8 mg g(-1) for Pb(II), 21.2 mg g(-1) for Zn(II), 19.5 mg g(-1) for Cu(II), and 15.7 mg g(-1) for Cd(II). The experiments demonstrated that the removal of metal ions followed the pseudo-second-order kinetic model. Desorption experiments were carried out using HCl solution with a view to regenerate the spent adsorbent and to recover the adsorbed metal ions.

  3. Removal of some metal ions by activated carbon prepared from Phaseolus aureus hulls

    International Nuclear Information System (INIS)

    Rao, M. Madhava; Ramana, D.K.; Seshaiah, K.; Wang, M.C.; Chien, S.W. Chang

    2009-01-01

    Removal of lead [Pb(II)], zinc [Zn(II)], copper [Cu(II)], and cadmium [Cd(II)] from aqueous solutions using activated carbon prepared from Phaseolus aureus hulls (ACPAH), an agricultural waste was studied. The influence of various parameters such as effect of pH, contact time, adsorbent dose, and initial concentration of metal ions on the removal was evaluated by batch method. The removal of metal ions by ACPAH was pH dependent and the optimum pH values were 7.0, 8.0, 7.0 and 6.0 for Cu(II), Cd(II), Zn(II), and Pb(II), respectively. The sorption isotherms were studied using Langmuir, Freundlich, Dubinin-Radushkevich (D-R), and Temkin isotherm models. The maximum adsorption capacity values of ACPAH for metal ions were 21.8 mg g -1 for Pb(II), 21.2 mg g -1 for Zn(II), 19.5 mg g -1 for Cu(II), and 15.7 mg g -1 for Cd(II). The experiments demonstrated that the removal of metal ions followed the pseudo-second-order kinetic model. Desorption experiments were carried out using HCl solution with a view to regenerate the spent adsorbent and to recover the adsorbed metal ions.

  4. Nitrogen cycling in a 15N-fertilized bean (Phaseolus vulgaris L.) crop

    International Nuclear Information System (INIS)

    Victoria, R.L.; Libardi, P.L.; Reichardt, K.; Cervellini, A.

    1982-01-01

    To increase our understanding of the fate of applied nitrogen in Phaseolus vulgaris crops grown under tropical conditions, 15 N-labelled urea was applied to bean crops and followed for three consecutive cropping periods. Each crop received 100 kg urea-N ha - 1 and 41 kg KCl-K ha - 1 . At the end of each period we estimated each crop's recovery of the added nitrogen, the residual effects of nitrogen from the previous cropping period, the distribution of nitrogen in the soil profile, and leaching losses of nitrogen. In addition, to evaluate potential effects of added phosphorus on nitrogen cycling in this crop, beans were treated at planting with either 35 kg rock-phosphate-P, 35 kg superphosphate-P, or 0 kg P ha - 1 . Results showed that 31.2% of the nitrogen in the first crop was derived from the applied urea, which represents a nitrogen utilization efficiency of 38.5%, 6.2% of the nitrogen in the second crop was derived from fertilizer applied to the first crop, and 1.4% of the nitrogen in the third crop. (orig./AJ)

  5. Characterisation of a haemagglutinin from Hokkaido red bean (Phaseolus vulgaris cv. Hokkaido red bean).

    Science.gov (United States)

    Wong, Jack H; Wan, Chung T; Ng, Tzi B

    2010-01-15

    A haemagglutinin was purified from Japanese Hokkaido red beans (Phaseolus vulgaris cv. Hokkaido red bean) with a procedure that included three chromatographic media. Haemagglutinating activity was adsorbed on DEAE cellulose, Affi-gel blue gel and Mono S. The pure haemagglutinin was a homodimer and each subunit was around 30 kDa in molecular mass. The haemagglutinating activity of this agglutinin could not be inhibited by a variety of simple sugars at 200 mmol L(-1) concentration including alpha-L-fucose, D(+)-galactose, D(+)-glucose, D(+)-glucosamine, D(-)galactosamine, galacturonic acid, (+)-lactose, D(+)-melibose, L(-)-mannose, D(+)-mannose, D-mannosamine, D(+)-raffinose, L-rhamnose, (+)-xylose and galacturonic acid. The haemagglutinating activity was fully retained at pH 4-11 and at 0-80 degrees C, but was completely lost at extreme pH values (0-2 and 13-14) and at very high temperatures (90 degrees C and 100 degrees C). The haemagglutinin exhibited a weak mitogenic activity toward mouse splenocytes, a stronger anti-proliferative activity than Con A toward HepG2 (human hepatoma) cells and inhibited >80% of HIV-1 reverse transcriptase inhibitory activity at 3.3 micromol L(-1). It was devoid of anti-fungal activity. Hokkaido red bean haemagglutinin possesses a potent anti-proliferative effect on HepG2 cells. Copyright (c) 2009 Society of Chemical Industry.

  6. Carbon partitioning among leaves, fruits, and seeds during development of Phaseolus vulgaris L

    International Nuclear Information System (INIS)

    Geiger, D.R.; Shieh, Wenjang; Saluke, R.M.

    1989-01-01

    Development of vegetative and floral buds was found to be a key factor in establishing the way carbon is distributed among growing leaves and fruits in Phaseolus vulgaris L. plants. Leaves emerged principally during a period 14 to 32 days after planting while flowers were produced during a 10- to 12-day period near the end of leaf emergence. Timing of anthesis established the sigmoidal time course for dry weight accumulated by the composite of all fruits on the plant. During the first 12 days following anthesis, fruit growth mainly consisted of elongation and dry weight accumulation by the pod wall. Thereafter, seed dry weight increased for about 1 week, decreased markedly for several days, and then increased again over the next 2 weeks. Accumulation of imported carbon in individual seeds, measured by steady-state labeling, confirmed the time course for dry weight accumulation observed during seed development. Seed respiration rate initially increased rapidly along with dry weight and then remained nearly steady until seed maturation. A number of developmental events described in the literature coincided with the different phases of diauxic growth. The results demonstrated the feasibility of relating current rates of carbon import in individual seeds measured with tracer 14 C to the rates of conversion of imported sucrose and use of the products for specific developmental processes. The resulting data are useful for evaluating the roles of conversion and utilization of imported sucrose in regulating import by developing seeds

  7. Weed Interference Effects on Leaves, Internode and Harvest Index of Dry Bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Hossein GHAMARI

    2015-03-01

    Full Text Available The development of appropriate weed management strategies and efficient use of herbicides relies upon understanding weed-crop interactions. A field study was carried out to assess the effect of weed interference on leaves, internode and harvest index of dry bean (Phaseolus vulgaris L.. The experiment was established under a randomized complete block design with two types of weed interference treatments: plots with weeds and plots without weeds at different time intervals (0, 10, 20, 30, 40 and 50 days after crop emergence. The sigmoid Boltzmann model was used to quantify the crop traits as influenced by weed interference. Prolonged delays in weed removal reduced gradually the number of leaves of the crop. Weed interference decreased dry weight of leaves as well, so that the lowest value of it (33.49 g plant-1 was observed in full season during weed-infested treatment. Infestation of weeds affected the length of the crop internodes. While the weed interference duration increased, the length of the internodes decreased. Harvest index was also sensitive to weed competition. As the crop was kept weed-infested from the emergence for increasing periods of time, harvest index decreased to a value of 28.01%. A significant negative correlation between total biomass of weeds and dry bean traits (number of leaves, leaves dry weight, internode length and harvest index was observed. Therefore, weeds are able to adversely affect dry bean growth through constraining environmental resources and impairing leaves as the photosynthetic areas.

  8. Attempts to induce mutants resistant or tolerant to golden mosaic virus in dry beans (Phaseolus vulgaris)

    International Nuclear Information System (INIS)

    Tulmann Neto, A.; Ando, A.; Costa, A.S.

    1977-01-01

    The golden mosaic of dry beans (Phaseolus vulgaris L.) that is present in the tropical parts of the American continent has become a major hindrance for the cultivation of this food legume of great importance to many Latin America countries. Good control measures are not known and bean germ plasm resistant or tolerant to this virus disease is not yet available. Attempts to induce bean mutants with this desirable characteristic were made using gamma radiation and chemical mutagen. Some M 2 plants from one progeny of the cultivar Carioca treated with 0.48% ethyl methane sulphonate (EMS), 6 hours of treatment at 20 0 C, showed milder symptoms than the control progenies, and at the same time they showed a tendency to recover. This mutant is being tested under field conditions and used in crosses with other bean types that show a certain degree of tolerance, aiming at adding the favourable characters of both parents. Seeds of the hybrids, as well as those of the parent types, are also being further submitted to mutagenic treatments in order to obtain still better mutants that will be satisfactory for direct or indirect control of bean golden mosaic. (author)

  9. Growth Response of Two Phaseolus mungo L. Cultivars Induced by Arbuscular Mycorrhizal Fungi and Trichoderma viride

    Directory of Open Access Journals (Sweden)

    Navnita Sharma

    2016-01-01

    Full Text Available The present investigation aimed to quantify the difference in response of two Phaseolus mungo L. cultivars (i.e., UH-1 and IPU-94-1 to Glomus mosseae (G, that is, Funneliformis mosseae, Acaulospora laevis (A, and Trichoderma viride (T, in different combinations or alone. All the treatments were inoculated with Bradyrhizobium japonicum to ensure nodulation as soil used in the experiment was sterilized. After 120 days of inoculation, plants were analyzed for chlorophyll content, nodulation, mycorrhization, leaf area, and protein content. Results indicate variation in growth response of two cultivars with different treatments. Triple inoculation of plants with G + A + T proved to be the best treatment for growth followed by G + T in both cultivars. Our work allowed the selection of P. mungo L. cultivar UH-1 as highly mycorrhizal responsive as compared to IPU-94-1 and G. mosseae to be an efficient bioinoculant as compared to A. laevis for growth enhancement of P. mungo. Further characterization of P. mungo genotypes will enhance our knowledge of physiological and genetic mechanism behind increase in plant growth and yield due to AM symbiosis.

  10. Evidence for the endophytic colonization of Phaseolus vulgaris (common bean) roots by the diazotroph Herbaspirillum seropedicae.

    Science.gov (United States)

    Schmidt, M A; Souza, E M; Baura, V; Wassem, R; Yates, M G; Pedrosa, F O; Monteiro, R A

    2011-03-01

    Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was confirmed by amplification and sequencing of the 16SrRNA gene. Furthermore, confocal microscopy of P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris. Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris.

  11. Nutritional and water effect on fluoride uptake and respiration of bean seedlings. [Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Applegate, H G; Adams, D F

    1960-01-01

    Bean plants (Phaseolus vulgaris) were grown in an atmosphere containing 2.0 +/- 0.21 g F /mT (1.6 ppb). The effect of N, P, K, Fe, and Ca deficiencies and the effect of osmotic pressures of 0, 1.5, 3.0, 4.5, 6.0 and 7.5 pounds on fluoride uptake and fluoride-mediated respiration were studied. The data showed that P deficient plants took up more fluoride than plants deficient in any of the other elements studied. Fluoride-mediated respiration was phosphorous dependent, however. Plants low in Fe or K showed increased uptake of fluoride. Nitrogen had no effect on fluoride uptake under the conditions of this experiment. Plants low in Fe showed inhibition of oxygen uptake. This inhibition was accentuated by fluoride. The interactions of N, K and Ca with fluoride on respiration were complex. Neither fluoride uptake nor fluoride-mediated respiration appeared to be linked directly to the water economy of the plants. 14 references, 6 tables.

  12. Manganese toxicity effects on nodulation and nitrogen fixation of beans (Phaseolus vulgaris L. ), in acid soils

    Energy Technology Data Exchange (ETDEWEB)

    Doebereiner, J

    1966-02-01

    Three greenhouse experiments were conducted to study manganese toxicity effects on the nitrogen fixing symbiosis of beans (Phaseolus vulgaris). Addition of 40 ppm of manganese to two acid soils affected nodulation and nitrogen fixation. Dependent on the Rhizobion strain either nodule numbers or efficiency in nitrogen fixation were reduced; the efficiency of one Rhizobium-host combination was more affected than another. Under less severe conditions of manganese toxicity, reduction of nodule numbers or of efficiency in nitrogen fixation could be compensated by an increase of nodule size. In the absence of manganese toxicity nodulation and nitrogen fixation of beans were abundant in a soil with pH 4.4. Naturally occurring manganese toxicity in a gray hydromorphic soil was eliminated by liming. The total nitrogen content of bean plants which were dependent on symbiotic nitrogen fixation decreased linearly with the logarithm of the manganese concentration in the plants. This did not happen when the plants were grown with mineral nitrogen. The role of manganese toxicity in the well known sensitivity to acid soil conditions of certain legumes and the importance of selection of manganese tolerant Rhizobium strains for the inoculation of beans in acid tropical soils, are discussed. 25 references, 1 figure, 6 tables.

  13. The fate of urea applied to tropical bean (Phaseolus vulgaris, L.) crops

    International Nuclear Information System (INIS)

    Cervellini, A.; Libardi, P.L.; Victoria, R.L.; Reichardt, K.

    The fate of nitrogen is studied when it is applied to three bean (Phaseolus vulgaris, L.) crops variety 'carioca' grown on a site of 'Terra Roxa Estruturada' (Paleudalf) soil. Urea labeled with three different 15 N enrichment percentages was used in order to estimate crop recovery of N (and its utilization efficiency), residual effects of N from one crop to another, distribution of N in the soil profile after cropping and leaching losses of N. The superphosphate and the rockphosphate 'Araxa' were also used. Grain yield was not significantly different between the phosphorus treatments, indicating that both P sources behaved similarly. Differences in fertilizer 15 N enrichment did not affect calculated amounts of nitrogen derived from fertilizer and N utilization efficiency (NUE), as expected. The first crop recovered on the average 31,2% of the N from the applied urea. The second crop recovered 6,2% N from the fertilizer applied to the first crop. The third crop recovered only 1,4%. Taking in account the NUE for the three crops, they recovered 44,1% of the N applied to the first crop. The partition of nitrogen applied to the first crop in four components (crop N removal; soil mineral N (NO 3 + NH 4 ); soil organic N and leaching N) is analysed. Due to the low N utilization efficiency of the crop, much of N remains in the soil profile, being potentially available for leaching and so contributing for fertilizer pollution of ground water. (M.A.) [pt

  14. Chemical profile of beans cultivars (Phaseolus vulgaris) by 1H NMR - high resolution magic angle spinning (HR-MAS);Perfil quimico de cultivares de feijao (Phaseolus vulgaris) pela tecnica de high resolution magic angle spinning (HR-MAS)

    Energy Technology Data Exchange (ETDEWEB)

    Liao, Luciano Morais; Choze, Rafael; Cavalcante, Pedro Paulo Araujo; Santos, Suzana da Costa; Ferri, Pedro Henrique, E-mail: luciano@quimica.ufg.b [Universidade Federal de Goias (UFG), Goiania, GO (Brazil). Inst. de Quimica; Ferreira, Antonio Gilberto [Universidade Federal de Sao Carlos (UFScar), SP (Brazil). Dept. de Quimica

    2010-07-01

    The application of one-dimensional proton high-resolution magic angle spinning ({sup 1}H HR-MAS) NMR combined with a typical advantages of solid and liquid-state NMR techniques was used as input variables for the multivariate statistical analysis. In this paper, different cultivars of beans (Phaseolus vulgaris) developed and in development by EMBRAPA - Arroz e Feijao were analyzed by {sup 1}H HR-MAS, which have been demonstrated to be a valuable tool in its differentiation according chemical composition and avoid the manipulation of the samples as used in other techniques. (author)

  15. Colorado Fathers' Resource Guide = Guia de Recursos para los Padres en Colorado.

    Science.gov (United States)

    Colorado Foundation for Families and Children, Denver.

    Developed through the Colorado Fatherhood Connection, this guide, in English- and Spanish-language versions, provides suggestions and resources for fathers as well as tips on discipline, communication, and activities fathers can do with their children. Topics addressed in the guide include characteristics of responsible fatherhood, characteristics…

  16. 75 FR 58426 - Notice of Inventory Completion: The Colorado College, Colorado Springs, CO; Correction

    Science.gov (United States)

    2010-09-24

    ... Counsel, The Colorado College c/o Jan Bernstein, President, Bernstein & Associates - NAGPRA Consultants... responsible for notifying the Hopi Tribe of Arizona; Navajo Nation, Arizona, New Mexico & Utah; Ohkay Owingeh, New Mexico; Pueblo of Acoma, New Mexico; Pueblo of Cochiti, New Mexico; Pueblo of Isleta, New Mexico...

  17. Mise au point d'une technique de culture in vitro d'embryons immatures de Phaseolus

    Directory of Open Access Journals (Sweden)

    Véronique Schmit

    1997-01-01

    Full Text Available Development of an in vitro culture technique for immature Phaseolus embryos. In the interspecific crosses Phaseolus polyanthus (or P. coccineus (i? x P. vulgaris, the hybrid embryos abort very early. Therefore, it is essentiel to develop an in vitro culture technique that allows the rescue of beau embryos at globular or early heart-shaped stages. After several trials conceming the salts composition, the sugar rate and the amino acid concentration of différent in vitro culture media, a technique has been developed for heart-shaped Phaseolus embryos. This technique consists of two stages. In a first step, embryos are cultivated under darkness until their germination on a medium containing the salts of Gamborg et al. (1968, 400 mg . 1` (5mM - 1 -' NHNO,, 1 mg . 1-' thiamine HCI, 5 mg . l` nicotinic acid, 0.5 mg - l` pyridoxine, 1,000 mg . l` -glutamine, 1,000 mg . l` casein hydrolysate, 100 mg . l` myo-inositol, 0.028 mg . P N6-benzyladenine, 30 g . l` sucrase, and 8 g -1-' DIFCO agar. After germination, the embryos are cultivated under light on a second medium that does not contain any NHNO, complément and is poorer in amino acids (100 mg • 1-' L-glutamine. Developed with six deys old heart-shaped embryos of the P. vulgaris Bico de Ouro (NI 637 variety, this technique has proved its efficiency with other P. vulgaris and P. polyanthus génotypes. It allows an average régénération rate of 30% from the total number of cultivated embryos.

  18. Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania.

    Directory of Open Access Journals (Sweden)

    Noora Nordenstedt

    Full Text Available Common bean (Phaseolus vulgaris is an annual grain legume that was domesticated in Mesoamerica (Central America and the Andes. It is currently grown widely also on other continents including Africa. We surveyed seedborne viruses in new common bean varieties introduced to Nicaragua (Central America and in landraces and improved varieties grown in Tanzania (eastern Africa. Bean seeds, harvested from Nicaragua and Tanzania, were grown in insect-controlled greenhouse or screenhouse, respectively, to obtain leaf material for virus testing. Equal amounts of total RNA from different samples were pooled (30-36 samples per pool, and small RNAs were deep-sequenced (Illumina. Assembly of the reads (21-24 nt to contiguous sequences and searches for homologous viral sequences in databases revealed Phaseolus vulgaris endornavirus 1 (PvEV-1 and PvEV-2 in the bean varieties in Nicaragua and Tanzania. These viruses are not known to cause symptoms in common bean and are considered non-pathogenic. The small-RNA reads from each pool of samples were mapped to the previously characterized complete PvEV-1 and PvEV-2 sequences (genome lengths ca. 14 kb and 15 kb, respectively. Coverage of the viral genomes was 87.9-99.9%, depending on the pool. Coverage per nucleotide ranged from 5 to 471, confirming virus identification. PvEV-1 and PvEV-2 are known to occur in Phaseolus spp. in Central America, but there is little previous information about their occurrence in Nicaragua, and no information about occurrence in Africa. Aside from Cowpea mild mosaic virus detected in bean plants grown from been seeds harvested from one region in Tanzania, no other pathogenic seedborne viruses were detected. The low incidence of infections caused by pathogenic viruses transmitted via bean seeds may be attributable to new, virus-resistant CB varieties released by breeding programs in Nicaragua and Tanzania.

  19. Centauri High School Teacher Honored as Colorado Outstanding Biology

    Science.gov (United States)

    Teacher Centauri High School Teacher Honored as Colorado Outstanding Biology Teacher For more information contact: e:mail: Public Affairs Golden, Colo., May 2, 1997 -- Tracy Swedlund, biology teacher at Centauri High School in LaJara, was selected as Colorado's 1997 Outstanding Biology Teacher and will be

  20. Extensive Green Roof Research Program at Colorado State University

    Science.gov (United States)

    In the high elevation, semi-arid climate of Colorado, green roofs have not been scientifically tested. This research examined alternative plant species, media blends, and plant interactions on an existing modular extensive green roof in Denver, Colorado. Six plant species were ev...

  1. Colorado River cutthroat trout: a technical conservation assessment

    Science.gov (United States)

    Michael K. Young

    2008-01-01

    The Colorado River cutthroat trout (Oncorhynchus clarkii pleuriticus) was once distributed throughout the colder waters of the Colorado River basin above the Grand Canyon. About 8 percent of its historical range is occupied by unhybridized or ecologically significant populations. It has been petitioned for listing under the Endangered Species Act...

  2. 77 FR 21803 - Notice of Competitive Coal Lease Sale, Colorado

    Science.gov (United States)

    2012-04-11

    ..., COC-70615] Notice of Competitive Coal Lease Sale, Colorado AGENCY: Bureau of Land Management, Interior... described below in Gunnison County, Colorado, will be offered for competitive lease by sealed bid in accordance with the provisions of the Mineral Leasing Act of 1920, as amended. DATES: The lease sale will be...

  3. 77 FR 40630 - Notice of Competitive Coal Lease Sale, Colorado

    Science.gov (United States)

    2012-07-10

    ..., COC-74219] Notice of Competitive Coal Lease Sale, Colorado AGENCY: Bureau of Land Management, Interior... in the Wadge Seam described below in Routt County, Colorado, will be offered for competitive lease by sealed bid in accordance with the provisions of the Mineral Leasing Act of 1920, as amended. DATES: The...

  4. Gamma radiation effects on some nutritional and physico-chemical characteristics of stored beans (Phaseolus vulgaris L.)

    International Nuclear Information System (INIS)

    Mancini Filho, J.

    1990-01-01

    The radiation effects on physico-chemical and nutritional characteristics of three Brazilian varieties of beans (Phaseolus vulgaris, L.) - Catu, Rajado and Carioca -were studied. The analytical parameters were obtained by the determination of soaking and cooking times, biological value in rats, protein electrophoretic profile, reductors sugars, oligosaccharides, fiber and fatty acids content. Also, amyloglucosidase, phytohemagglutinins, α-amylase and tryptic inhibitors activities were analysed. It was observed the gamma radiation until determined doses promotes changes on those parameters subsequently reducing substantially the cooking time without modification of the biological value of the proteins. This alteration was particularly noticed in the hard-to-cook beans. (author)

  5. Improvement of protein and amino acid contents in seeds of food legumes. A case study in Phaseolus

    Directory of Open Access Journals (Sweden)

    Baudoin J.P.

    1999-01-01

    Full Text Available Food legumes are considered as the major source of dietary proteins among the plant species. Protein and amino acid contents were evaluated in a wide sample of both wild and cultivated genotypes of Phaseolus species, with a view to investigate possibilities of genetic improvement in seed nutritional quality. Results indicate a variation in relation with taxa, biological status within species (such as in P. lunatus, ecological conditions, seed parts (testa, cotyledons and embryonic axis, and major protein groups. However, the sulphur containing amino acids remain a limiting factor, which could be better overcome by mixing food legumes with other plant species such as cereals.

  6. Correlation of protein content to flatulent galactooligosaccharides and exogenous amino acids in seeds of Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ryszard Kosson

    2014-01-01

    Full Text Available In order to estimate the possible correlations among constituents of Phaseolus vulgaris seeds, the contents of protein, exogenous amino acids and flatulent galactooligosaccharides (raffinose and stachyose were analyzed in 16 Polish bean cultivars for dry seeds. A negative correlation coefficient (r =-0.9490 was found between protein and methionine contents. High positive correlations among exogenous amino acids, such as lysine and isoleucine, valine and isoleucine, lysine and tyrosine, were observed indicating a chance of selecting far more than one at a time. The small-seeded bean cultivars contained higher contents of protein and galactooligosaccharides than the large-seeded ones.

  7. Development of industrial minerals in Colorado

    Science.gov (United States)

    Arbogast, Belinda F.; Knepper, Daniel H.; Langer, William H.; Cappa, James A.; Keller, John W.; Widmann, Beth L.; Ellefsen, Karl J.; Klein, Terry L.; Lucius, Jeffrey E.; Dersch, John S.

    2011-01-01

    Technology and engineering have helped make mining safer and cleaner for both humans and the environment. Inevitably, mineral development entails costs as well as benefits. Developing a mine is an environmental, engineering, and planning challenge that must conform to many Federal, State, and local regulations. Community collaboration, creative design, and best management practices of sustainability and biodiversity can be positive indicators for the mining industry. A better understanding of aesthetics, culture, economics, geology, climate, vegetation and wildlife, topography, historical significance, and regional land planning is important in resolving land-use issues and managing mineral resources wisely. Ultimately, the consuming public makes choices about product use (including water, food, highways, housing, and thousands of other items) that influence operations of the mineral industry. Land planners, resource managers, earth scientists, designers, and public groups have a responsibility to consider sound scientific information, society's needs, and community appeals in making smart decisions concerning resource use and how complex landscapes should change. An effort to provide comprehensive geosciences data for land management agencies in central Colorado was undertaken in 2003 by scientists of the U.S. Geological Survey and the Colorado Geological Survey. This effort, the Central Colorado Assessment Project, addressed a variety of land-use issues: an understanding of the availability of industrial and metallic rocks and minerals, the geochemical and environmental effects of historic mining activity on surface water and groundwater, and the geologic controls on the availability and quality of groundwater. The USDA Forest Service and other land management agencies have the opportunity to contribute to the sustainable management of natural aggregate and other mineral resources through the identification and selective development of mineral resources and the

  8. Cancer incidence study in Mesa County, Colorado

    International Nuclear Information System (INIS)

    Ouimette, D.R.; Ferguson, S.W.; Zoglo, D.; Murphy, S.; Alley, S.; Bahler, S.

    1983-01-01

    In November of 1982 the Colorado Department of Health completed an epidemiologic investigation of leukemia, multiple myeloma, and cancers of the lung, stomach, pancreas and colon in Mesa County, Colorado for the years 1970 to 1979. This investigation was performed in response to a concern that the presence of uranium mill tailings in some Mesa County homes presents a potential cancer hazard. The results of the investigation show that the incidence of multiple myeloma, colon, stomach and pancreatic cancer are not above expected rates. The incidence of leukemia is not above expected rates for the entire study period, 1970 to 1979. The incidence of lung cancer appears elevated when compared to the The Third National Cancer Survey data for Colorado but lower than expected when compared to Surveillance, Epidemiology and End Results data. To further examine the leukemia and lung cancer incidence findings, a case/control study was conducted. The controls consisted of colon, stomach and pancreatic cancer cases. The results of the leukemia case/control analysis show no association with the radiation exposure variables: occupational radiation exposure; uranium mining exposure; having ever lived in a type A home (uranium tailings home); and radiation therapy. The lung cancer case/control analysis shows a significant association with only the radiation exposure variable, uranium mining history, indicating cases were more likely to have been uranium miners than were controls. As with leukemia, the study found no association between lung cancer and living in a uranium mill tailings home. The relatively low radiation exposures typical of type A homes and the small number of persons exposed make it very difficult to establish, by epidemiologic methods, that a risk exists

  9. Mount Zirkel Wilderness and vicinity, Colorado

    International Nuclear Information System (INIS)

    Snyder, G.L.; Patten, L.L.

    1984-01-01

    Several areas of metallic and nonmetallic mineralization have been identified from surface occurrences within the Mount Zirkel Wilderness and vicinity, Colorado. Three areas of probable copper-lead-zinc-silver-gold resource potential, two areas of probable chrome-platinum resource potential, four areas of probable uranium-thorium resource potential, two areas of probable molybdenum resource potential, and one area of probable fluorspar potential were identified by studies in 1965-1973 by the USGS and USBM. No potential for fossil fuel or geothermal resources was identified

  10. Evaluación agronómica de líneas avanzadas de fríjol voluble Phaseolus vulgaris L. en Paipa, Boyacá

    Directory of Open Access Journals (Sweden)

    Diana Katherine Ríos

    2014-06-01

    Full Text Available En Boyacá la oferta de germoplasma de frijol mejorado es baja. El objetivo de este estudio fue evaluar el comportamiento agronómico de 18 líneas avanzadas volubles de frijol, procedentes del CIAT. Se utilizó el diseño bloques completos al azar, con tres repeticiones, incluyendo Bola Roja, Bolívar y Cargamanto como testigos. Se evaluó días a floración, vigor de crecimiento, días a madurez, incidencia de enfermedades, número de vainas/planta, número de granos/vaina, peso de 100 granos y rendimiento. Los genotipos MAC27, MAC13, MBC26, MBC28 y MBC37 no se diferenciaron del Cargamanto pero sí mostraron ser más precoces que Bola Roja y Bolívar. En días a madurez MAC27 y MBC39 fueron más tempranos que los testigos. Todos los genotipos se comportaron como resistentes a enfermedades, excepto MBC30 que resultó susceptible a Uromyces phaseoli y MBC33 a Phoma exigua. En número de vainas por planta, número de granos por vaina, peso de 100 semillas y rendimiento se destacaron MAC27 y LAS399, sin diferencia significativa frente a Cargamanto pero si superando a Bola Roja y Bolívar. Pese al efecto de las heladas en la fase final del ciclo, LAS399, MAC27 y Cargamanto produjeron rendimientos de 1300, 1038 y 950 kg/ha, respectivamente, mostrando las ventajas de su precocidad, en comparación a Bola Roja y Bolívar que son tardíos. Se concluye que LAS399 y MAC27, por su alta precocidad y resistencia a patógenos, son las líneas más promisorias para continuar la evaluación respecto a la posibilidad de ampliar la oferta de germoplasma de frijol voluble en Boyacá.

  11. Adaptação do feijoeiro comum (Phaseolus vulgaris L. à seca Common bean (Phaseolus vulgaris L. adaptation to drought

    Directory of Open Access Journals (Sweden)

    Cleber M. Guimarães

    2006-03-01

    Full Text Available Realizou-se este trabalho tendo em vista o propósito de se estudar a adaptação de genótipos de feijoeiro comum (Phaseolus vulgaris L. à seca, como suporte a programas de melhoramento que visem à criação de cultivares para regiões sujeitas a deficiência hídrica, mediante a avaliação do potencial da água nas folhas e da resistência difusiva estomática. Adicionalmente avaliou-se a técnica de medição da temperatura do dossel pela termometria de infravermelho para inferir o estado hídrico da planta. Os genótipos Carioca e RAB 96 foram submetidos, dos 20 dias após a emergência até a colheita, a dois tratamentos hídricos: irrigação adequada e com deficiência hídrica. Em geral, a cultivar Carioca manteve potenciais de água na folha mais altos e também melhor capacidade de recuperação hídrica, além de apresentar resistência difusiva estomática e temperatura do dossel mais baixas que a linhagem RAB 96 sendo, portanto, melhor adaptada à seca. A temperatura do dossel correlacionou-se significativamente com o potencial da água nas folhas e, devido a sua medição ser rápida e não-destrutiva, mostrou tratar-se de uma técnica útil no processo de seleção de genótipos para regiões sujeitas a deficiência hídrica.The objective of this study was to investigate the common bean adaptation to drought, as support to breeding programs directed to regions with water deficiency, through the evaluation of leaf water potential and stomatal diffusive resistance. Furthermore, the canopy temperature was evaluated with the infrared thermometer technique to infer the plant water content. From twenty days after emergence until harvest, the genotypes Carioca and RAB 96 were submitted to two water treatments: adequate irrigation and water deficit. The 'Carioca' showed higher leaf water potentials and better water recovery capacity compared with 'RAB 96'. This cultivar also showed lower stomatal diffusive resistance and canopy

  12. KARAKTERISASI ENAM VARIETAS BUNCIS (Phaseolus vulgaris L. BERDASARKAN PANDUAN PENGUJIAN INDIVIDUAL

    Directory of Open Access Journals (Sweden)

    Vina Eka Aristya

    2016-02-01

    Full Text Available Beans (Phaseolus vulgaris L. is a plant that has the potential for mainstream consumers, have a large enough market opportunity and a source of vegetable protein. “Perancis” varieties are local Central Java bean varieties are widely grown in the Bandungan area. “Perancis” varieties not currently provide enough characters clear and complete. Test objectives were (1 to characterize the “Perancis” varieties in order to have a complete character information varieties and (2 to determine distinctness, uniformity and stability of the “Perancis” varieties compared with varieties Gypsie, Spectacular, Balitsa 1, Balitsa 2 and PV 072 using guidelines for the conduct of test for distinctness, uniformity and stability reference beans. Implemented on the Garden Seed Testing Bandungan Horticulture, Central Java with an altitude of 560-800 meters above sea level the place. Materials testing consists of six varieties of beans are “Perancis” varieties and the varieties used for comparison Gypsie, Spectacular, Balitsa 1, Balitsa 2 and PV 072. This research used a randomized block design six varieties of beans are planted side by side on three experimental plots as replications. Parameters observed include 49 characters corresponding guidelines bean plants are divided into the plant character, leaves character, flower character, pods character and seed character. Test results based guidelines, “Perancis” varieties showed eight unique characters compared to varieties of Gypsie, Spectacular, Balitsa 1, Balitsa 2 and PV 072 ie. plant height, leaf color, leaf rugosity, long (including beak pods, the degree of the pods curvature, the shape of distal part (excluding beak pods, length of beak pods and curvature of beak pods and “Perancis” varieties have uniformity and stability.

  13. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris.

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    Grady H Zuiderveen

    Full Text Available Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L. caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS. Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481 included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219 tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  14. Effects of putrescine, kinetin and IAA on protein synthesis in 'Phaseolus vulgaris' coleoptiles

    Energy Technology Data Exchange (ETDEWEB)

    Crocomo, O J; Lee, T S.G. [Centro de Energia Nuclear na Agricultura, Piracicaba (Brazil)

    1975-01-01

    Incubation of etiolated 'Phaseolus vulgaris' coleoptiles shows a converse flux between soluble protein and reducing sugar. The rate of incorporation of radioactive arginine into protein was higher than that of radioactive leucine. Radioactive arginine incorporation into protein was linear up to 120 min and then started to decline. The rate of incorporation of radioactive leucine was increased by preincubation of the tissue in the incubation medium. Roots were found to contain more soluble protein and much less reducing sugar than the coleoptile. The optimum pH value for protein synthesis in coleoptile sections was found to be 6 for control tissues and 4 for those treated with 10-/sup 3/M IAA. This high concentration of IAA was also found to inhibit soluble protein synthesis, the incorporation rate of radioactive arginine and leucine into protein fraction, the secretion of hydrogen ion into the incubation medium and elongation of the bean segment. Kinetin at 2x10/sup -4/M and putrescine at 5mM both decreased the rate of /sup 14/C-arginine incorporation into soluble protein, but for /sup 14/C-leucine, this rate of incorporation was found to be increased after 90 min incubation with a preincubation of 30 min. In general, the change pattern of the soluble protein content, the reducing sugar level and the incorporation rate of radioactive arginine and leucine into protein in the kinetin and putrescine treated tissues were about the same although tissues that incubated with kinetin always contain more soluble protein and less reducing sugar than that of incubated with putrescine.

  15. Physical, biochemical and physiological effects of ultraviolet radiation on Brassica napus and Phaseolus vulgaris

    International Nuclear Information System (INIS)

    Cen Yan-Ping.

    1993-01-01

    In order to follow some of the changes induced by ultraviolet-B (UV-B, 280-320 nm) radiation in Phaseolus vulgaris and Brassica napus, experiments were designed to localize sites of changes in leaves and to correlate some of the physiological and biochemical changes with penetration of UV-B radiation. B.napus was exposed to 8.9 kJ m -2 day -1 biologically effective UV-B radiation (UV-B BE ). The penetration of UV-B radiation into the leaf was followed using a quartz fibre optic microprobe. Monochromatic radiation at 310 nm was decreased by ca 50 and 34% in the adaxial and abaxial epidermis, respectively, in plants not exposed to UV-B, whereas the radiation was decreased by ca 70 and 42%, respectively, in the same region in UV-treated plants. Polychromatic radiation showed a wavelength dependent change mainly for the collimated radiation. The results correlated with the distribution of phenolic compounds analysed from 40 μm paradermal leaf sections. The first adaxial section (40μm) contained 35% of the whole leaf sample flavonoid glycosides in control plants, and 66% in UV-treated plants. Hydroxycinnamic acid derivatives increased by 26% in UV-treated plants relative to controls. The ratio of quercetin to kaempferol derivatives increased from 0.11 in controls to 0.91 in leaves of UV-treated plants. The leaf epidermis protected the inner leaf tissue where most of the photosynthetic apparatus is located. P. vulgaris was subjected to 6.17 kJ m -2 day -1 UV-B BE with different levels of visible light. The largest UV-induced changes in photosynthesis, chlorophyll, carotenoids, UV-screening pigments, and surface leaf reflectance occurred under growth conditions of low levels of visible light together with UV radiation

  16. Transcriptome analysis of salt tolerant common bean (Phaseolus vulgaris L. under saline conditions.

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    Mahmut Can Hiz

    Full Text Available Salinity is one of the important abiotic stress factors that limit crop production. Common bean, Phaseolus vulgaris L., a major protein source in developing countries, is highly affected by soil salinity and the information on genes that play a role in salt tolerance is scarce. We aimed to identify differentially expressed genes (DEGs and related pathways by comprehensive analysis of transcriptomes of both root and leaf tissues of the tolerant genotype grown under saline and control conditions in hydroponic system. We have generated a total of 158 million high-quality reads which were assembled into 83,774 all-unigenes with a mean length of 813 bp and N50 of 1,449 bp. Among the all-unigenes, 58,171 were assigned with Nr annotations after homology analyses. It was revealed that 6,422 and 4,555 all-unigenes were differentially expressed upon salt stress in leaf and root tissues respectively. Validation of the RNA-seq quantifications (RPKM values was performed by qRT-PCR (Quantitative Reverse Transcription PCR analyses. Enrichment analyses of DEGs based on GO and KEGG databases have shown that both leaf and root tissues regulate energy metabolism, transmembrane transport activity, and secondary metabolites to cope with salinity. A total of 2,678 putative common bean transcription factors were identified and classified under 59 transcription factor families; among them 441 were salt responsive. The data generated in this study will help in understanding the fundamentals of salt tolerance in common bean and will provide resources for functional genomic studies.

  17. Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

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    Martina Lardi

    2017-12-01

    Full Text Available Paraburkholderia phymatum belongs to the β-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papilionoid legumes. In contrast to the symbiosis of legumes with α-proteobacteria, very little is known about the molecular determinants underlying the successful establishment of this mutualistic relationship with β-proteobacteria. In this study, we performed an RNA-sequencing (RNA-seq analysis of free-living P. phymatum growing under nitrogen-replete and -limited conditions, the latter partially mimicking the situation in nitrogen-deprived soils. Among the genes upregulated under nitrogen limitation, we found genes involved in exopolysaccharides production and in motility, two traits relevant for plant root infection. Next, RNA-seq data of P. phymatum grown under free-living conditions and from symbiotic root nodules of Phaseolus vulgaris (common bean were generated and compared. Among the genes highly upregulated during symbiosis, we identified—besides the nif gene cluster—an operon encoding a potential cytochrome o ubiquinol oxidase (Bphy_3646-49. Bean root nodules induced by a cyoB mutant strain showed reduced nitrogenase and nitrogen fixation abilities, suggesting an important role of the cytochrome for respiration inside the nodule. The analysis of mutant strains for the RNA polymerase transcription factor RpoN (σ54 and its activator NifA indicated that—similar to the situation in α-rhizobia—P. phymatum RpoN and NifA are key regulators during symbiosis with P. vulgaris.

  18. Impact of management strategies in the basal rot, charcoal rots epidemiology and Phaseolus vulgaris L. yield.

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    Ulacio Osorio Dilcia

    2013-02-01

    Full Text Available The effect of chemical, physical, biologycal and cultural strategies individually or combinated were evaluated in the epidemiology of the basal rot (Sclerotium rolfsii, charcoal rot (Macrophomina phaseolina and the Phaseolus vulgaris cv Tacarigua yield at Barinas state from Venezuela. In the experiment, Tebuconazole (Teb was applicated at seed (1 L/Ton and at soil, a los 30 y 60 days after of the sow (1 L/ha; Trichoderma harzianum (Tri was applicated at seed (15 g for each 1.5 k and to 15, 30, 45 y 60 days after of the sow (30 g/10 L of water. On the other hand, soil was solarizated (Sol during 15 days and calcium nitrate (Ca (60 g/10 L of water was applicated each 15 days until 60 days of growth of cultivated plants. Basal rot was registered as far as 42 days after of the sow, showing less of 5.3% in Teb y the combination SolTeb. The hightest incidence of this disease was observed in the treatment Tri with 28.5%, being highter that control (14.5%. Last to 42 days predominated the charcoal rot in the rest of the plants for a total of 100% of incidente in everything the treatments. Nevertheless, Teb showed the hightest yield with 555 k/ha, being different estatistically at treatment TriCa, which showed the lowest yield with 31 k/ha, however, the roots not formed nodules nitrogen uptake in these replications with the fungicide and Ca. It is concluded that S. rolfsii was sensible at action of some of the treatments; but not M. phaseolina; nevertheless, the plants were capables to produce seeds health apparently in treatments in which observed less severity of charcoal rot.

  19. Anatomical alterations of Phaseolus vulgaris L. mature leaves irradiated with X-rays.

    Science.gov (United States)

    De Micco, V; Arena, C; Aronne, G

    2014-01-01

    The cultivation of higher plants in Space involves not only the development of new agro-technologies for the design of ecologically closed Space greenhouses, but also understanding of the effects of Space factors on biological systems. Among Space factors, ionising radiation is one of the main constraints to the growth of organisms. In this paper, we analyse the effect of low-LET radiation on leaf histology and cytology in Phaseolus vulgaris L. plants subjected to increasing doses of X-rays (0.3, 10, 50, 100 Gy). Leaves irradiated at tissue maturity were compared with not-irradiated controls. Semi-thin sections of leaves were analysed through light and epi-fluorescence microscopy. Digital image analysis was applied to quantify anatomical parameters, with a specific focus on the occurrence of signs of structural damage as well as alterations at subcellular level, such as the accumulation of phenolic compounds and chloroplast size. Results showed that even at high levels of radiation, general anatomical structure was not severely perturbed. Slight changes in mesophyll density and cell enlargement were detected at the highest level of radiation. However, at 100 Gy, higher levels of phenolic compounds accumulated along chloroplast membranes: this accompanied an increase in number of chloroplasts. The reduced content of chlorophylls at high levels of radiation was associated with reduced size of the chloroplasts. All data are discussed in terms of the possible role of cellular modifications in the maintenance of high radioresistance and photosynthetic efficiency. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.

  20. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris).

    Science.gov (United States)

    Zuiderveen, Grady H; Padder, Bilal A; Kamfwa, Kelvin; Song, Qijian; Kelly, James D

    2016-01-01

    Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L.) caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS). Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481) included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL) for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL) population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219) tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  1. Physical, biochemical and physiological effects of ultraviolet radiation on Brassica napus and Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Cen Yan-Ping

    1993-12-31

    In order to follow some of the changes induced by ultraviolet-B (UV-B, 280-320 nm) radiation in Phaseolus vulgaris and Brassica napus, experiments were designed to localize sites of changes in leaves and to correlate some of the physiological and biochemical changes with penetration of UV-B radiation. B.napus was exposed to 8.9 kJ m{sup -2} day{sup -1} biologically effective UV-B radiation (UV-B{sub BE}). The penetration of UV-B radiation into the leaf was followed using a quartz fibre optic microprobe. Monochromatic radiation at 310 nm was decreased by ca 50 and 34% in the adaxial and abaxial epidermis, respectively, in plants not exposed to UV-B, whereas the radiation was decreased by ca 70 and 42%, respectively, in the same region in UV-treated plants. Polychromatic radiation showed a wavelength dependent change mainly for the collimated radiation. The results correlated with the distribution of phenolic compounds analysed from 40 {mu}m paradermal leaf sections. The first adaxial section (40{mu}m) contained 35% of the whole leaf sample flavonoid glycosides in control plants, and 66% in UV-treated plants. Hydroxycinnamic acid derivatives increased by 26% in UV-treated plants relative to controls. The ratio of quercetin to kaempferol derivatives increased from 0.11 in controls to 0.91 in leaves of UV-treated plants. The leaf epidermis protected the inner leaf tissue where most of the photosynthetic apparatus is located. P. vulgaris was subjected to 6.17 kJ m{sup -2} day{sup -1} UV-B{sub BE} with different levels of visible light. The largest UV-induced changes in photosynthesis, chlorophyll, carotenoids, UV-screening pigments, and surface leaf reflectance occurred under growth conditions of low levels of visible light together with UV radiation.

  2. Mechanistic insight into softening of Canadian wonder common beans (Phaseolus vulgaris) during cooking.

    Science.gov (United States)

    Chigwedere, Claire Maria; Olaoye, Taye Foyeke; Kyomugasho, Clare; Jamsazzadeh Kermani, Zahra; Pallares Pallares, Andrea; Van Loey, Ann M; Grauwet, Tara; Hendrickx, Marc E

    2018-04-01

    The relative contributions of cotyledons and seed coats towards hardening of common beans (Phaseolus vulgaris) were investigated and the rate-limiting process which controls bean softening during cooking was determined. Fresh or aged whole beans and cotyledons were soaked and cooked in demineralised water or 0.1 M NaHCO 3 solution, and texture evolution, microstructure changes and thermal properties were studied. Fresh and aged whole beans cooked in demineralised water had significantly different softening rate constants and so did fresh and aged cotyledons. The comparable softening rate constants of aged whole beans and cotyledons indicated an insignificant role of the seed coat in hardening during storage. All samples cooked faster in 0.1 M NaHCO 3 solution. Disintegration of cooked tissues followed by microscopic examination revealed a transition from cell breakage through a phase of cell breakage and separation to complete cell separation with increased cooking time wherefore texture decayed. Therefore, progressive solubilization of pectin in the middle lamella greatly promoted texture decay. While residual birefringence even after substantial cooking time suggested some molecular order of the starch, calorimetric analyses revealed complete starch gelatinisation before complete cell separation occurred. This implies an insignificant role of starch in texture decay during cooking but its hindered uncoiling into a viscous gel after gelatinisation due to the restricting cell wall could promote its retrogradation. Therefore, we suggest that the rate-determining process in bean softening relates to cell wall/middle lamella changes influencing pectin solubilization. Copyright © 2018 Elsevier Ltd. All rights reserved.

  3. Annotation and sequence diversity of transposable elements in common bean (Phaseolus vulgaris

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    Scott eJackson

    2014-07-01

    Full Text Available Common bean (Phaseolus vulgaris is an important legume crop grown and consumed worldwide. With the availability of the common bean genome sequence, the next challenge is to annotate the genome and characterize functional DNA elements. Transposable elements (TEs are the most abundant component of plant genomes and can dramatically affect genome evolution and genetic variation. Thus, it is pivotal to identify TEs in the common bean genome. In this study, we performed a genome-wide transposon annotation in common bean using a combination of homology and sequence structure-based methods. We developed a 2.12-Mb transposon database which includes 791 representative transposon sequences and is available upon request or from www.phytozome.org. Of note, nearly all transposons in the database are previously unrecognized TEs. More than 5,000 transposon-related expressed sequence tags (ESTs were detected which indicates that some transposons may be transcriptionally active. Two Ty1-copia retrotransposon families were found to encode the envelope-like protein which has rarely been identified in plant genomes. Also, we identified an extra open reading frame (ORF termed ORF2 from 15 Ty3-gypsy families that was located between the ORF encoding the retrotransposase and the 3’LTR. The ORF2 was in opposite transcriptional orientation to retrotransposase. Sequence homology searches and phylogenetic analysis suggested that the ORF2 may have an ancient origin, but its function is not clear. This transposon data provides a useful resource for understanding the genome organization and evolution and may be used to identify active TEs for developing transposon-tagging system in common bean and other related genomes.

  4. Response of French Bean (Phaseolus vulgaris L. Cultivars to Foliar Applications of Magnesium

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    Michele Pisante

    2011-02-01

    Full Text Available Magnesium deficiencies have been shown to be particularly dangerous to short cycled crops, both on sandy and clay soils. Such deficiencies may be corrected by foliar fertilisations, but in French bean (Phaseolus vulgaris L. no experimental data may be found to support this hypothesis. Therefore this paper was aimed at studying the effect of foliar Mg-applications (56, 112 and 224 g ha-1 in single application at flowering or splitted half dose at 4-leaf stage and half at flowering alone and with Zn (200 g ha-1 on yield and quality of two French bean genotypes (Bronco, Cadillac. Foliar Mg-applications significantly increased pod yield and, considering the highest rate with respect to the untreated, such an increase was 78% and 32% for Bronco and Cadillac, respectively. Split applications were also more effective, with yield increases of 109% and 50% for the two genotypes. Concerning quality, foliar Mg applications showed a significant effect particularly on sugars, calcium, phosphate, sulphate and Mg contents in pods. On the other hand, a significant effect on the accumulation of nitrates was noted, especially with split applications (144% increase vs. unfertilised and, in some cases, an antagonistic effect on K content (10-20% decrease on average. Foliar Mg fertilisation of French bean seemed to be a promising practice with reference to human health and nutrition, tough some care is needed to avoid the accumulation of nitrates in pods. Split applications seemed to be more effective, while the addition of Zn to the fertiliser mix did not give any relevant effect.

  5. Radioactive mineral occurences of Colorado and bibliography

    International Nuclear Information System (INIS)

    Nelson-Moore, J.L.; Collins, D.B.; Hornbaker, A.L.

    1978-01-01

    This two-part report provides an essentially complete listing of radioactive occurrences in Colorado, with a comprehensive bibliography and bibliographic cross-indexes. Part 1 lists approximately 3000 known radioactive occurrences with their locations and brief accounts of the geology, mineralogy, radioactivity, host rock, production data, and source of data for each. The occurrences are classified by host rock and plotted on U.S. Geological Survey 1 0 x 2 0 topographic quadrangle maps with a special 1 : 100,000-scale base map for the Uravan mineral belt. Part 2 contains the bibliography of approximately 2500 citations on radioactive mineral occurrences in the state, with cross-indexes by county, host rock, and the special categories of ''Front Range,'' ''Colorado Plateau,'' and ''thorium.'' The term ''occurrence'' as used in this report is defined as any site where the concentration of uranium or thorium is at least 0.01% or where the range of radioactivity is greater than twice the background radioactivity. All citations and occurrence data are stored on computer diskettes for easy retrieval, correction, and updating

  6. National uranium resource evaluation, Montrose Quadrangle, Colorado

    International Nuclear Information System (INIS)

    Goodknight, C.S.; Ludlam, J.R.

    1981-06-01

    The Montrose Quadrangle in west-central Colorado was evaluated to identify and delineate areas favorable for the occurrence of uranium deposits according to National Uranium Resource Evaluation program criteria. General surface reconnaissance and geochemical sampling were conducted in all geologic environments in the quadrangle. Preliminary data from aerial radiometric and hydrogeochemical and stream-sediment reconnaissance were analyzed and brief followup studies were performed. Twelve favorable areas were delineated in the quadrangle. Five favorable areas contain environments for magmatic-hydrothermal uranium deposits along fault zones in the Colorado mineral belt. Five areas in parts of the Harding and Entrada Sandstones and Wasatch and Ohio Creek Formations are favorable environments for sandstone-type uranium deposits. The area of late-stage rhyolite bodies related to the Lake City caldera is a favorable environment for hydroauthigenic uranium deposits. One small area is favorable for uranium deposits of uncertain genesis. All near-surface Phanerozoic sedimentary rocks are unfavorable for uranium deposits, except parts of four formations. All near-surface plutonic igneous rocks are unfavorable for uranium deposits, except five areas of vein-type deposits along Tertiary fault zones. All near-surface volcanic rocks, except one area of rhyolite bodies and several unevaluated areas, are unfavorable for uranium. All near-surface Precambrian metamorphic rocks are unfavorable for uranium deposits. Parts of two wilderness areas, two primitive areas, and most of the subsurface environment are unevaluated

  7. Epidemiologic characterization of Colorado backyard bird flocks.

    Science.gov (United States)

    Smith, Emily I; Reif, John S; Hill, Ashley E; Slota, Katharine E; Miller, Ryan S; Bjork, Kathe E; Pabilonia, Kristy L

    2012-06-01

    Backyard gallinaceous bird flocks may play an important role in the spread of infectious diseases within poultry populations as well as the transmission of zoonotic diseases to humans. An epidemiologic characterization was conducted of Colorado backyard flocks to gather information on general flock characteristics, human movement of birds, human-bird interaction, biosecurity practices, and flock health. Our results suggest that backyard poultry flocks in Colorado are small-sized flocks (68.6% of flocks had meat or egg) production for the family (86.44%) or as pet or hobby birds (42.27%). The backyard flock environment may promote bird-to-bird transmission as well as bird-to-human transmission of infectious disease. Birds are primarily housed with free access to the outside (96.85%), and many are moved from the home premises (46.06% within 1 yr). Human contact with backyard flocks is high, biosecurity practices are minimal, and bird health is negatively impacted by increased movement events. Increased knowledge of backyard bird characteristics and associated management practices can provide guidelines for the development of measures to decrease disease transmission between bird populations, decrease disease transmission from birds to humans, and increase the overall health of backyard birds.

  8. Improving adaptation to drought stress in white pea bean (Phaseolus vulgaris L): genotypic effects on grain yield, yield components and pod harvest index

    Science.gov (United States)

    Common bean (Phaseolus vulgaris L.) is the most important food legume crop in Africa and Latin America where rainfall pattern is unpredictable. The objectives were to identify better yielding common bean lines with good canning quality under drought, and to identify traits that could be used as sele...

  9. Molecular characterization of the U.S. Phaseolus acutifolius A. Gray collection using Amplified Fragment Length Polymorphism (AFLP) and Targeted Region Amplification Polymorphism (TRAP) markers.

    Science.gov (United States)

    Tepary bean (Phaseolus acutifolius A. Gray), a truly Native American crop, is a short life-cycle annual desert legume indigenous to northwestern Mexico and the southwestern USA and is considered drought and heat tolerant. The Western Regional Plant Introduction Station currently maintains 211 acce...

  10. Effects of water stress on the photosynthetic assimilation and distribution of 14C-photosynthate in maize (Zea mays L.) and bean (Phaseolus vulgaris L.)

    International Nuclear Information System (INIS)

    Martinez y Huaman, C.A.; Cerri, C.C.

    1984-01-01

    The relationship between photosynthesis and distribution of 14 C-photosinthate as affected by water stress was evaluated. Corn (Zea mays L.) during the grain filling period and bean (Phaseolus vulgaris L.) during flowering, representing a C-4 and a C-3 photosynthetic type, respectively, were studied. (M.A.C.) [pt

  11. Assesing potential effects of inulin and probiotic bacteria on Fe bioavailability from common beans (Phaseolus vulgaris L.) to Caco-2 cells

    Science.gov (United States)

    Inulin, a prebiotic, may enhance intestinal Fe absorption. Our objective was to assess the effects of supplemental inulin and two probiotic bacteria (B. infantis and L.acidophillus) on Fe availability to Caco-2 cells from common white and red beans (Phaseolus vulgaris L.). Cooked beans were mixed o...

  12. Improovement of statistical counting conditions for the determination of chloride in Beams leaves (Phaseolus vulgaris L.) by thermal neutron activation analysis

    International Nuclear Information System (INIS)

    Ferraz, E.S.B.; Nascimento Filho, V.F.

    1975-04-01

    The use of two radiation peaks from the same gamma-emitting source in the calculation of the corresponding liquid counting rate in multi-element gamma spectrometry is discussed. It is shown that, in the determination of chlorine in Phaseolus vulgaris L. using neutronic activation analysis will result in an increase in accuracy of measurement of approximately 40%

  13. La collection de base des espèces sauvages de Phaseolus et Vigna : historique, gestion et conservation

    Directory of Open Access Journals (Sweden)

    Thierry Vanderborght

    1998-01-01

    Full Text Available The base collection of wild species of Phaseolus and Vigna: history, management and conservation.The National Botanic Garden of Belgium ensures the management of a base collection of botanical and wild forms in the tribe Phaseoleae and the sub-tribe Phaseolinae. The main objective is to conserve on a long terni basic the largest possible genetic diversity through seed semples stored at - 20°C. The collection provided the basic material for the investigations conducted at the University Faculty of Agricultural Sciences of Gembloux in fields as diverse as taxonomy, genome analysis, definition of genetic réservoirs, agronomie and chemical evaluations, interspecific hybridization and plant breeding. The results have allowed to becter understand the organization of genetic diversity in the studied plant material and to highlight the wealthy genetic potentiel of the collection. The latter should be preserved and valorized for the genetic improvement of food legumes, in particular within the two genera Phaseolus and Vigna.

  14. Environmental Setting and Implications on Water Quality, Upper Colorado River Basin, Colorado and Utah

    Science.gov (United States)

    Apodaca, Lori E.; Driver, Nancy E.; Stephens, Verlin C.; Spahr, Norman E.

    1995-01-01

    The Upper Colorado River Basin in Colorado and Utah is 1 of 60 study units selected for water-quality assessment as part of the U.S. Geological Survey's National Water-Quality Assessment program, which began full implementation in 1991. Understanding the environmental setting of the Upper Colorado River Basin study unit is important in evaluating water-quality issues in the basin. Natural and human factors that affect water quality in the basin are presented, including an overview of the physiography, climatic conditions, general geology and soils, ecoregions, population, land use, water management and use, hydrologic characteristics, and to the extent possible aquatic biology. These factors have substantial implications on water-quality conditions in the basin. For example, high concentrations of dissolved solids and selenium are present in the natural background water conditions of surface and ground water in parts ofthe basin. In addition, mining, urban, and agricultural land and water uses result in the presence of certain constituents in the surface and ground water of the basin that can detrimentally affect water quality. The environmental setting of the study unit provides a framework of the basin characteristics, which is important in the design of integrated studies of surface water, ground water, and biology.

  15. Ponnequin Wind Energy Project Weld County, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-08-01

    The purpose of this environmental assessment (EA) is to provide the U.S. Department of Energy (DOE) and the public with information on potential environmental impacts associated with the development of the Ponnequin Wind Energy Project in Colorado. This EA and public comments received on it will be used in DOE`s deliberations on whether to release funding for the project. This document provides a detailed description of the proposed project and an assessment of potential impacts associated with its construction and operations. Resources and conditions considered in the analysis include streams; wetlands; floodplains; water quality; soils; vegetation; air quality; socioeconomic conditions; energy resources; noise; transportation; cultural resources; visual and land use resources; public health and safety; wildlife; threatened, endangered, and candidate species; and cumulative impacts. The analysis found that the project would have minimal impacts on these resources and conditions, and would not create impacts that exceed the significance criteria defined in this document. 90 refs., 5 figs.

  16. Uranium indicator plants of the Colorado plateau

    International Nuclear Information System (INIS)

    Massingill, G.L.

    1979-01-01

    Two methods of botanical prospecting for uranium deposits have been applied on the Colorado Plateau. The first, based on a chemical analysis of deep-rooted plants that absorb uranium from ore bodies, detects small but measurable amounts of the element in plants rooted in ore. A second method involves mapping the distribution of indicator plants because these plants are dependent--either directly or indirectly--upon the presence of abnormally high levels of elements in the parent soil or rock. Botanical prospecting studies made in ten districts have been productive. In the Thompson district, Grand County, Utah, five ore bodies were found solely on the basis of indicator plant data. 15 refs

  17. Upper Colorado River Basin Climate Effects Network

    Science.gov (United States)

    Belnap, Jayne; Campbell, Donald; Kershner, Jeff

    2011-01-01

    The Upper Colorado River Basin (UCRB) Climate Effects Network (CEN) is a science team established to provide information to assist land managers in future decision making processes by providing a better understanding of how future climate change, land use, invasive species, altered fire cycles, human systems, and the interactions among these factors will affect ecosystems and the services they provide to human communities. The goals of this group are to (1) identify science needs and provide tools to assist land managers in addressing these needs, (2) provide a Web site where users can access information pertinent to this region, and (3) provide managers technical assistance when needed. Answers to the team's working science questions are intended to address how interactions among climate change, land use, and management practices may affect key aspects of water availability, ecosystem changes, and societal needs within the UCRB.

  18. SPANISH PEAKS WILDERNESS STUDY AREA, COLORADO.

    Science.gov (United States)

    Budding, Karin E.; Kluender, Steven E.

    1984-01-01

    A geologic and geochemical investigation and a survey of mines and prospects were conducted to evaluate the mineral-resource potential of the Spanish Peaks Wilderness Study Area, Huerfano and Las Animas Counties, in south-central Colorado. Anomalous gold, silver, copper, lead, and zinc concentrations in rocks and in stream sediments from drainage basins in the vicinity of the old mines and prospects on West Spanish Peak indicate a substantiated mineral-resource potential for base and precious metals in the area surrounding this peak; however, the mineralized veins are sparse, small in size, and generally low in grade. There is a possibility that coal may underlie the study area, but it would be at great depth and it is unlikely that it would have survived the intense igneous activity in the area. There is little likelihood for the occurrence of oil and gas because of the lack of structural traps and the igneous activity.

  19. Thesis Abstract Bean (Phaseolus vulgaris L.) lines: chemical composition and protein digestibility.

    Science.gov (United States)

    Mesquita, F R; Silva, M I A; Corrêa, A D

    2016-05-09

    The bean represents the main source of proteins for the low income populations, although the digestibility of those proteins is relatively low. Consequently, the programs of plant genetic breeding have been working on the search for new lines with higher protein levels. Thus, with the purpose of supplying information to the researchers, in this study, 21 bean (Phaseolus vulgaris L.) lines were analyzed for the centesimal and mineral composition, protein digestibility, phenolic compounds, and trypsin inhibitor. The entirely randomized experimental design was used with 21 treatments (lines) and three repetitions. All values were within the following ranges: 22.34 to 36.28 g crude protein/100 g dry matter (DM); 7.56 to 20.91 g neutral detergent fiber/100 g DM; 0.53 to 2.55 g fat/100 g DM and 2.97 to 4.87 g ashes/100 g DM. The levels of phosphorus, potassium, calcium, magnesium, and sulfur, in g/100 g DM, varied from 0.45 to 0.72; 1.51 to 2.48; 0.03 to 0.28; 0.18 to 0.34 and 0.28 to 0.45, respectively. Regarding copper, manganese, zinc and iron, the levels, in mg/kg DM, varied from 11.37 to 17.73; 14.93 to 28.90; 36.67 to 69.90 and 71.37 to 126.90, respectively. The in vitro protein digestibility varied from 18.03 to 48.32%. The levels of phenolic compounds varied from 0.28 to 1.08 mg acid tanic/100 g DM and the one of trypsin inhibitor from 59.93 to 151.07 trypsin inhibited units/mg DM. Among the lines with higher protein contents, "ESAL 569" (beige with brown stripe) presented the largest protein digestibility and considerable levels of minerals. "P-180" (beige with brown stripe) was one of the lines with higher crude protein contents and digestibilities, and also presented high levels for most of the minerals. No relation between protein digestibility and the contents of phenolic compounds or trypsin inhibitor was observed.

  20. Domestication Genomics of the Open-Pollinated Scarlet Runner Bean (Phaseolus coccineus L.)

    Science.gov (United States)

    Guerra-García, Azalea; Suárez-Atilano, Marco; Mastretta-Yanes, Alicia; Delgado-Salinas, Alfonso; Piñero, Daniel

    2017-01-01

    The runner bean is a legume species from Mesoamerica closely related to common bean (Phaseolus vulgaris). It is a perennial species, but it is usually cultivated in small-scale agriculture as an annual crop for its dry seeds and edible immature pods. Unlike the common bean, P. coccineus has received little attention from a genetic standpoint. In this work we aim to (1) provide information about the domestication history and domestication events of P. coccineus; (2) examine the distribution and level of genetic diversity in wild and cultivated Mexican populations of this species; and, (3) identify candidate loci to natural and artificial selection. For this, we generated genotyping by sequencing data (42,548 SNPs) from 242 individuals of P. coccineus and the domesticated forms of the closely related species P. vulgaris (20) and P. dumosus (35). Eight genetic clusters were detected, of which half corresponds to wild populations and the rest to domesticated plants. The cultivated populations conform a monophyletic clade, suggesting that only one domestication event occurred in Mexico, and that it took place around populations of the Trans-Mexican Volcanic Belt. No difference between wild and domesticated levels of genetic diversity was detected and effective population sizes are relatively high, supporting a weak genetic bottleneck during domestication. Most populations presented an excess of heterozygotes, probably due to inbreeding depression. One population of P. coccineus subsp. striatus had the greatest excess and seems to be genetically isolated despite being geographically close to other wild populations. Contrasting with previous studies, we did not find evidence of recent gene flow between wild and cultivated populations. Based on outlier detection methods, we identified 24 domestication-related SNPs, 13 related to cultivar diversification and eight under natural selection. Few of these SNPs fell within annotated loci, but the annotated domestication

  1. Domestication Genomics of the Open-Pollinated Scarlet Runner Bean (Phaseolus coccineus L.

    Directory of Open Access Journals (Sweden)

    Azalea Guerra-García

    2017-11-01

    Full Text Available The runner bean is a legume species from Mesoamerica closely related to common bean (Phaseolus vulgaris. It is a perennial species, but it is usually cultivated in small-scale agriculture as an annual crop for its dry seeds and edible immature pods. Unlike the common bean, P. coccineus has received little attention from a genetic standpoint. In this work we aim to (1 provide information about the domestication history and domestication events of P. coccineus; (2 examine the distribution and level of genetic diversity in wild and cultivated Mexican populations of this species; and, (3 identify candidate loci to natural and artificial selection. For this, we generated genotyping by sequencing data (42,548 SNPs from 242 individuals of P. coccineus and the domesticated forms of the closely related species P. vulgaris (20 and P. dumosus (35. Eight genetic clusters were detected, of which half corresponds to wild populations and the rest to domesticated plants. The cultivated populations conform a monophyletic clade, suggesting that only one domestication event occurred in Mexico, and that it took place around populations of the Trans-Mexican Volcanic Belt. No difference between wild and domesticated levels of genetic diversity was detected and effective population sizes are relatively high, supporting a weak genetic bottleneck during domestication. Most populations presented an excess of heterozygotes, probably due to inbreeding depression. One population of P. coccineus subsp. striatus had the greatest excess and seems to be genetically isolated despite being geographically close to other wild populations. Contrasting with previous studies, we did not find evidence of recent gene flow between wild and cultivated populations. Based on outlier detection methods, we identified 24 domestication-related SNPs, 13 related to cultivar diversification and eight under natural selection. Few of these SNPs fell within annotated loci, but the annotated

  2. Effect of vermicompost on soil fertility and crop productivity--beans (Phaseolus vulgaris).

    Science.gov (United States)

    Manivannan, S; Balamurugan, M; Parthasarathi, K; Gunasekaran, G; Ranganathan, L S

    2009-03-01

    Field experiments were conducted at Sivapuri, Chidambaram, Tamil Nadu to evaluate the efficacy of vermicompost, in comparison to inorganic fertilizers-NPK, on the physio-chemical and biological characteristics of the soils--clay loam soil (CLS) and sandy loam soil (SLS) and on the growth, yield and nutrient content of beans--Phaseolus vulgaris. Results showed that the application of vermicompost @ 5 tonnes ha(-1) had enhanced significantly the pore space (1.09 and 1.02 times), water holding capacity (1.1 and 1.3 times), cation exchange capacity (1.2 and 1.2 times). It reduced particles (1.2 and 1.2 times), and bulk density (1.2 and 1.2 times), pH (1 and 1.02 times) and electrical conductivity (1.4 and 1.2 times) and increased organic carbon (37 and 47 times), micro (Ca 3.07 and 1.9 times, Mg 1.6 and 1.6 times, Na 2.4 and 3.8 times, Fe 7 and 7.6 times, Mn 8.2 and 10.6 times, Zn 50 and 52 times and Cu 14 and 22 times) and macro (N 1.6 and 1.7 times, P 1.5 and 1.7 times, K 1.5 and 1.4 times) nutrients and microbial activity (1.4 and 1.5 times) in both soil types, particularly more in CLS. The growth, yield (1.6 times) and quality (protein (1.05 times) and sugar (1.01 times) content in seed) of bean were enhanced in CLS than SLS. On the other hand, the application of inorganic fertilizers @ 20:80:40 kg ha(-1) has resulted in reduced porosity (1.03 and 1.01 times), organic carbon (1.04 and 9.5 times) and microbial activity (1.02 and 1.03 times) in both soil types.

  3. Does methyl jasmonate modify the oxidative stress response in Phaseolus coccineus treated with Cu?

    Science.gov (United States)

    Hanaka, Agnieszka; Wójcik, Małgorzata; Dresler, Sławomir; Mroczek-Zdyrska, Magdalena; Maksymiec, Waldemar

    2016-02-01

    The contribution of methyl jasmonate (MJ) as a signal molecule able to take part in the defense mechanism against copper (Cu)-imposed oxidative stress was studied in the leaves and roots of runner bean (Phaseolus coccineus) plants. Roots of plants cultivated hydroponically were preincubated in MJ (10µM) for 1h or 24h and subsequently exposed to Cu (50µM) for 5h (short-term experiment) or 5 days (long-term experiment). Enzymatic (activity of superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX; guaiacol peroxidase, POX) and non-enzymatic (accumulation of malondialdehyde, MDA; homoglutathione, hGSH; proline; anthocyanins; low molecular weight organic acids, LMWOAs) responses were determined in the leaves and roots. The antioxidative defense mechanism was significantly activated after Cu supplementation. In most cases, activities of ROS (reactive oxygen species) scavenging enzymes like SOD, CAT, APX, POX, as well as MDA, hGSH and proline concentrations increased following Cu exposure. MJ showed a time-dependent effect on antioxidative enzymes activity. In the short-term experiment, MJ elevated CAT, APX and POX activities in the roots, and POX activity in the leaves of non-Cu-treated plants. In the long-term experiment, MJ not only decreased POX and partially CAT activity in the roots, but also increased the MDA level and partially CAT activity in the leaves of the control plants. In Cu-treated plants, MJ reduced APX, but elevated POX activity in the leaves after 5-h exposure. After 5-day-Cu treatment, MJ inhibited POX activity in the leaves and mainly reduced SOD and CAT activities in the roots. Moreover, in the long-term experiment, MJ reduced tartrate and pyruvate in the leaves of Cu-stressed plants, but mostly elevated tartrate and malate in the roots comparing with Cu alone treatment. MJ alone and under Cu excess did not alter accumulation of MDA, hGSH and proline comparing with Cu alone, but partially elevated anthocyanin concentration. The

  4. Biosynthesis of compatible solutes in rhizobial strains isolated from Phaseolus vulgaris nodules in Tunisian fields

    Directory of Open Access Journals (Sweden)

    Nieto Joaquín J

    2010-07-01

    Full Text Available Abstract Background Associated with appropriate crop and soil management, inoculation of legumes with microbial biofertilizers can improve food legume yield and soil fertility and reduce pollution by inorganic fertilizers. Rhizospheric bacteria are subjected to osmotic stress imposed by drought and/or NaCl, two abiotic constraints frequently found in semi-arid lands. Osmostress response in bacteria involves the accumulation of small organic compounds called compatible solutes. Whereas most studies on rhizobial osmoadaptation have focussed on the model species Sinorhizobium meliloti, little is known on the osmoadaptive mechanisms used by native rhizobia, which are good sources of inoculants. In this work, we investigated the synthesis and accumulations of compatible solutes by four rhizobial strains isolated from root nodules of Phaseolus vulgaris in Tunisia, as well as by the reference strain Rhizobium tropici CIAT 899T. Results The most NaCl-tolerant strain was A. tumefaciens 10c2, followed (in decreasing order by R. tropici CIAT 899, R. leguminosarum bv. phaseoli 31c3, R. etli 12a3 and R. gallicum bv. phaseoli 8a3. 13C- and 1H-NMR analyses showed that all Rhizobium strains synthesized trehalose whereas A. tumefaciens 10c2 synthesized mannosucrose. Glutamate synthesis was also observed in R. tropici CIAT 899, R. leguminosarum bv. phaseoli 31c3 and A. tumefaciens 10c2. When added as a carbon source, mannitol was also accumulated by all strains. Accumulation of trehalose in R. tropici CIAT 899 and of mannosucrose in A. tumefaciens 10c2 was osmoregulated, suggesting their involvement in osmotolerance. The phylogenetic analysis of the otsA gene, encoding the trehalose-6-phosphate synthase, suggested the existence of lateral transfer events. In vivo 13C labeling experiments together with genomic analysis led us to propose the uptake and conversion pathways of different carbon sources into trehalose. Collaterally, the β-1,2-cyclic glucan from R

  5. Linkage disequilibrium at the APA insecticidal seed protein locus of common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Blair, Matthew W; Prieto, Sergio; Díaz, Lucy M; Buendía, Héctor F; Cardona, César

    2010-04-29

    An interesting seed protein family with a role in preventing insect herbivory is the multi-gene, APA family encoding the alpha-amylase inhibitor, phytohemagglutinin and arcelin proteins of common bean (Phaseolus vulgaris). Variability for this gene family exists and has been exploited to breed for insect resistance. For example, the arcelin locus has been successfully transferred from wild to cultivated common bean genotypes to provide resistance against the bruchid species Zabrotes subfasciatus although the process has been hampered by a lack of genetic tools for and understanding about the locus. In this study, we analyzed linkage disequilibrium (LD) between microsatellite markers at the APA locus and bruchid resistance in a germplasm survey of 105 resistant and susceptible genotypes and compared this with LD in other parts of the genome. Microsatellite allele diversity was found to vary with each of the eight APA-linked markers analyzed, and two markers within the APA locus were found to be diagnostic for bruchid resistance or susceptibility and for the different arcelin alleles inherited from the wild accessions. Arc1 was found to provide higher levels of resistance than Arc5 and the markers in the APA locus were highly associated with resistance showing that introgression of this gene-family from wild beans provides resistance in cultivated beans. LD around the APA locus was found to be intermediate compared to other regions of the genome and the highest LD was found within the APA locus itself for example between the markers PV-atct001 and PV-ag004. We found the APA locus to be an important genetic determinant of bruchid resistance and also found that LD existed mostly within the APA locus but not beyond it. Moderate LD was also found for some other regions of the genome perhaps related to domestication genes. The LD pattern may reflect the introgression of arcelin from the wild into the cultivated background through breeding. LD and association studies for

  6. Colorado's hydrothermal resource base: an assessment

    Energy Technology Data Exchange (ETDEWEB)

    Pearl, R.H.

    1981-01-01

    As part of its effort to more accurately describe the nations geothrmal resource potential, the US Department of Energy/Division of Geothermal Energy contracted with the Colorado Geological survey to appraise the hydrothermal (hot water) geothermal resources of Colorado. Part of this effort required that the amount of energy that could possibly be contained in the various hydrothermal systems in Colorado be estimated. The findings of that assessment are presented. To make these estimates the geothermometer reservoir temperatures estimated by Barrett and Pearl (1978) were used. In addition, the possible reservoir size and extent were estimated and used. This assessment shows that the total energy content of the thermal systems in Colorado could range from 4.872 x 10{sup 15} BTU's to 13.2386 x 10{sup 15} BTU's.

  7. BLM Colorado Oil and Gas Communitization Agreements (Dissolve)

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  8. BLM Colorado Oil and Gas Storage Agreements (Dissolve)

    Data.gov (United States)

    Department of the Interior — Shapefile Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  9. Evaluation of guardrail embedded lighting system in Trinidad, Colorado.

    Science.gov (United States)

    2014-02-01

    This report provides information on the design considerations of the embedded highway lighting : design on Interstate-25 in Trinidad, Colorado, in terms of visibility. The information is based on : visibility characterizations of small targets using ...

  10. BLM Colorado Oil and Gas Storage Agreements (Dissolve)

    Data.gov (United States)

    Department of the Interior — KMZ File Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  11. Effect of food factor on microevolution of Colorado beetle

    Directory of Open Access Journals (Sweden)

    N. А. Ryabchenko

    2005-12-01

    Full Text Available Many-sided research of interaction of Colorado beetle and fodder plant (potato, nightshade sweetly-bitter defines the role of the plants as guiding factor of microevolutional processes in pest population.

  12. Colorado River Sewer System Joint Venture to Upgrade Wastewater System

    Science.gov (United States)

    SAN FRANCISCO -Today, the Colorado River Sewer System Joint Venture, located in Parker, Ariz. entered into an agreement with the EPA to upgrade their wastewater treatment system to meet stringent water quality standards. The cost of the upgrade is ap

  13. BLM Colorado Oil and Gas Communitization Agreements (Dissolve)

    Data.gov (United States)

    Department of the Interior — KMZ File Format –This data is one feature type that is a part of a set consisting of six shapefiles pertaining to energy development and production in Colorado. The...

  14. University of Colorado Students Join Pros in Covering Columbine Incident.

    Science.gov (United States)

    Litherland, Chip

    1999-01-01

    Describes the experiences and feelings of a university photojournalist as he covered the shootings at Columbine High School in Littleton, Colorado. Notes the onslaught of the media presence and the overwhelming emotion he witnessed. (RS)

  15. Barriers to Enrollment in Health Coverage in Colorado.

    Science.gov (United States)

    Martin, Laurie T; Bharmal, Nazleen; Blanchard, Janice C; Harvey, Melody; Williams, Malcolm

    2015-03-20

    As part of the implementation of the Affordable Care Act, Colorado has expanded Medicaid and also now operates its own health insurance exchange for individuals (called Connect for Health Colorado). As of early 2014, more than 300,000 Coloradans have newly enrolled in Medicaid or health insurance through Connect for Health Colorado, but there also continues to be a diverse mix of individuals in Colorado who remain eligible for but not enrolled in either private insurance or Medicaid. The Colorado Health Foundation commissioned the RAND Corporation to conduct a study to better understand why these individuals are not enrolled in health insurance coverage and to develop recommendations for how Colorado can strengthen its outreach and enrollment efforts during the next open enrollment period, which starts in November 2014. RAND conducted focus groups with uninsured and newly insured individuals across the state and interviews with local stakeholders responsible for enrollment efforts in their regions. The authors identified 11 commonly cited barriers, as well as several that were specific to certain regions or populations (such as young adults and seasonal workers). Collectively, these barriers point to a set of four priority recommendations that stakeholders in Colorado may wish to consider: (1) Support and expand localized outreach and tailored messaging; (2) Strengthen marketing and messaging to be clear, focused on health benefits of insurance (rather than politics and mandates), and actionable; (3) Improve the clarity and transparency of insurance and health care costs and enrollment procedures; and (4) Revisit the two-stage enrollment process and improve Connect for Health Colorado website navigation and technical support.

  16. Tracking Water-Use in Colorado's Energy Exploration and Development

    Science.gov (United States)

    Halamka, T. A.; Ge, S.

    2017-12-01

    By the year 2050 Colorado's population is projected to nearly double, posing many important questions about the stresses that Colorado's water resources will experience. Growing in tandem with Colorado's population is the state's energy exploration and development industry. As water demands increase across the state, the energy exploration and development industry must adapt to and prepare for future difficulties surrounding the legal acquisition of water. The goal of this study is to map out the potential sources of water within the state of Colorado that are being purchased, or will be eligible for purchase, for unconventional subsurface energy extraction. The background of this study includes an overview of the intertwined relationship between water, the energy industry, and the Colorado economy. The project also aims to determine the original purpose of legally appropriated water that is used in Colorado's energy exploration and development. Is the water primarily being purchased or leased from the agricultural sector? Is the water mostly surface water or groundwater? In order to answer these questions, we accessed data from numerous water reporting agencies and examined legal methods of acquisition of water for use in the energy industry. Using these data, we assess the future water quantity available to the energy industry. Knowledge and foresight on the origins of the water used by the energy industry will allow for better and strategic planning of water resources and how the industry will respond to statewide water-related stresses.

  17. Economic impact study of the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado State fiscal year 1994. Revision 1

    International Nuclear Information System (INIS)

    1994-12-01

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year 1994 (1 July 1993 through 30 June 1994). To capture employment information, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Naturita, Gunnison, and Rifle, Colorado. Economic data were requested from each site prime subcontractor, as well as from the Remedial Action Contractor. Information on wages, taxes, and subcontract expenditures in combination with estimates and economic multipliers is used to estimate the dollar economic benefits to Colorado during the state fiscal year. Finally, the fiscal year 1994 estimates are compared to fiscal year 1993 employment and economic information

  18. Comparisons of phaseolin type and α-amylase inhibitor in common bean(Phaseolus vulgaris L.)in China

    Institute of Scientific and Technical Information of China (English)

    Yang Yao; Yibo Hu; Yingying Zhu; Yue Gao; Guixing Ren

    2016-01-01

    The objective of this study was to characterize the phaseolin type and a-amylase(αAI) level in common bean(Phaseolus vidgaris L.) accessions deposited in the Chinese National Genebank.The 40 accessions sampled were common varieties originating in Asia,North America,South America,Europe,and Africa.No Inca(I-) phaseolin was observed in the accessions.Only four accessions contained Tendergreen(T-) phaseolin and the remaining36 contained Sanilac(S-) phaseolin.aAI proteins extracted from nine accessions showed higher a-amylase inhibitory activity than the control(Phase 2,IC50 = 0.65 μg).These common bean accessions have potential use as nutraceutical ingredients.

  19. Purification and characterization of an alkaline phosphatase induced by phosphorus starvation in common bean (Phaseolus vulgaris L.) roots

    Energy Technology Data Exchange (ETDEWEB)

    Morales, L.; Gutierrez, N.; Maya, V.; Parra, C.; Martinez B, E.; Coello, P., E-mail: pcoello@servidor.unam.mx [UNAM, Facultad de Quimica, Departamento de Bioquimica, Ciudad Universitaria, 04510 Mexico D. F. (Mexico)

    2012-07-01

    Two phosphatase isoforms from roots of the common bean (Phaseolus vulgaris L.) showed an increase in activity in response to phosphate deficiency. One of them (APIII) was chosen for further purification through ionic exchange chromatography and preparative electrophoresis. The estimated molecular mass of APIII was 35 kDa by both SDS-Page and gel filtration analyses, suggesting a monomeric form of the active enzyme. The phosphatase was classified as an alkaline phosphatase based on the requirement of ph 8 for optimum catalysis. It not only exhibited broad substrate specificity, with the most activity against pyrophosphate, but also effectively catalyzed the hydrolysis of polyphosphate, glucose-1-phosphate and phospho enol-pyruvate. Activity was completely inhibited by molybdate, vanadate and phosphate but was only partially inhibited by fluoride. Although divalent cations were not essential for the pyro phosphatase activity of this enzyme, the hydrolysis of pyro phosphatase increased substantially in the presence of Mg{sup 2+}.

  20. Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba

    Directory of Open Access Journals (Sweden)

    Matthew G. Nosworthy

    2018-05-01

    Full Text Available In this work, the protein quality of different bean types after undergoing the preparatory methods of baking, cooking and extrusion was assayed. Protein quality was assessed using a rodent bioassay to evaluate growth and protein digestibility while amino acid composition was determined via HPLC. In vivo protein digestibility was compared to an in vitro assessment method. The average protein digestibility corrected amino acid score (PDCAAS for processed beans was higher than the digestible indispensable amino acid score (DIAAS (61% vs. 45%. Extrusion/cooking of Phaseolus varieties resulted in higher PDCAAS (66% on average and DIAAS values (61% on average than baked (52% and 48% while baked faba beans had higher PDCAAS (66% and DIAAS (61% values. A significant correlation was found between PDCAAS and in vitro PDCAAS (R2 = 0.7497. This demonstrates which bean processing method will generate the optimal protein quality, which has benefits for both industrial production and individual domestic preparation.

  1. Effect of cooking on aroma profile of red kidney beans (Phaseolus vulgaris) and correlation with sensory quality.

    Science.gov (United States)

    Mishra, Prashant K; Tripathi, Jyoti; Gupta, Sumit; Variyar, Prasad S

    2017-01-15

    Volatile aroma compounds of three varieties of red kidney beans (Phaseolus vulgaris) namely Kashmiri red, Sharmili and Chitra were extracted in raw state using solid-phase microextraction (SPME) and cooked state using simultaneous distillation extraction (SDE). During cooking a significant (palcohols and terpene hydrocarbons while an increase in content of various sulfurous compounds, terpene alcohols, ketones and pyrazines was noted. Descriptive sensory analysis showed that the maximum intensity of 'kidney bean', 'earthy' and 'smoky' odour was observed in Kashmiri red while Sharmili variety was characterised by 'sulfurous' odour. Correlation of volatile profile data with descriptive sensory analysis and odour activity values clearly established the role of compounds, such as methanethiol, diethyl sulfide, dimethyl disulfide, methional and dimethyl trisulfide, in contributing to 'cooked kidney bean' aroma, while dimethyl sulfoxide, dimethyl sulfone and ethyl methyl sulfone were responsible for 'sulfurous' aroma. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Pathovars of Pseudomonas syringae Causing Bacterial Brown Spot and Halo Blight in Phaseolus vulgaris L. Are Distinguishable by Ribotyping

    Science.gov (United States)

    González, Ana J.; Landeras, Elena; Mendoza, M. Carmen

    2000-01-01

    Ribotyping was evaluated as a method to differentiate between Pseudomonas syringae pv. phaseolicola and pv. syringae strains causing bacterial brown spot and halo blight diseases in Phaseolus vulgaris L. Ribotyping, with restriction enzymes BglI and SalI and using the Escherichia coli rrnB operon as the probe, differentiated 11 and 14 ribotypes, respectively, and a combination of data from both procedures yielded 19 combined ribotypes. Cluster analysis of the combined ribotypes differentiated the pathovars phaseolicola and syringae, as well as different clonal lineages within these pathovars. The potential of ribotyping to screen for correlations between lineages and factors such as geographical region and/or bean varieties is also reported. PMID:10653764

  3. Purification and characterization of an alkaline phosphatase induced by phosphorus starvation in common bean (Phaseolus vulgaris L.) roots

    International Nuclear Information System (INIS)

    Morales, L.; Gutierrez, N.; Maya, V.; Parra, C.; Martinez B, E.; Coello, P.

    2012-01-01

    Two phosphatase isoforms from roots of the common bean (Phaseolus vulgaris L.) showed an increase in activity in response to phosphate deficiency. One of them (APIII) was chosen for further purification through ionic exchange chromatography and preparative electrophoresis. The estimated molecular mass of APIII was 35 kDa by both SDS-Page and gel filtration analyses, suggesting a monomeric form of the active enzyme. The phosphatase was classified as an alkaline phosphatase based on the requirement of ph 8 for optimum catalysis. It not only exhibited broad substrate specificity, with the most activity against pyrophosphate, but also effectively catalyzed the hydrolysis of polyphosphate, glucose-1-phosphate and phospho enol-pyruvate. Activity was completely inhibited by molybdate, vanadate and phosphate but was only partially inhibited by fluoride. Although divalent cations were not essential for the pyro phosphatase activity of this enzyme, the hydrolysis of pyro phosphatase increased substantially in the presence of Mg 2+ .

  4. Studies on the distribution of 14C-malformin A in major fractions of Phaseolus vulgaris L

    International Nuclear Information System (INIS)

    Ciarlante, D.; Curtis, R.W.

    1976-01-01

    The distribution pattern of 14 C-malformin in major fractions of Phaseolus vulgaris L, seedlings shifted during water treatment in the absence of malformin. From these shifts, and by comparison of the 14 C distribution patterns at the base and top of the seedlings, it was concluded that some 14 C-malformin enters the cell and proceeds to the cell wall via intermediate compounds. As a working hypothesis it was suggested that in roots 14 C-malformin first appears in a soluble ''small molecules'' fraction, binds to a soluble protein fraction, and proceeds via the wall lipid fraction to the wall itself. Direct binding of some 14 C-malformin to the wall fraction was not precluded. In leaves, the pathway of 14 C-malformin to the cell wall was similar in some respects to that in roots. (auth.)

  5. Effects of acetylsalicylic acid on fresh weight pigment and protein content of bean leaf discs (Phaseolus vulgaris L.).

    Science.gov (United States)

    Canakçi, S

    2003-01-01

    The effects of 100, 250, and 500 ppm acetylsalicylic acid solutions treatments on weight alteration, pigment and protein amounts in discs from the primary leaves of one month old bean (Phaseolus vulgaris L.) seedlings produced tinder greenhouse conditions are presented. The experiments show that: 100 ppm ASA had no significant influence (P > 0.05) but 250 and 500 ppm ASA caused an increase on weight loss (P 0.05), none of the ASA treatments caused a statistically significant influence on carotenoid amount (P > 0.05); 100 and 250 ppm ASA treatments did not cause a significant influence on protein amount (P > 0.05). however 500 ppm ASA treatment caused an increase on protein injury (P < 0.05). Consequently, it is supposed that wet weight loss, pigment and protein injury have somewhat increased on leaf discs. depending on the toxic effect of high acetylsalicylic acid concentrations.

  6. Functional specialization of one copy of glutamine phosphoribosyl pyrophosphate amidotransferase in ureide production from symbiotically fixed nitrogen in Phaseolus vulgaris.

    Science.gov (United States)

    Coleto, Inmaculada; Trenas, Almudena T; Erban, Alexander; Kopka, Joachim; Pineda, Manuel; Alamillo, Josefa M

    2016-08-01

    Purines are essential molecules formed in a highly regulated pathway in all organisms. In tropical legumes, the nitrogen fixed in the nodules is used to generate ureides through the oxidation of de novo synthesized purines. Glutamine phosphoribosyl pyrophosphate amidotransferase (PRAT) catalyses the first committed step of de novo purine synthesis. In Phaseolus vulgaris there are three genes coding for PRAT. The three full-length sequences, which are intron-less genes, were cloned, and their expression levels were determined under conditions that affect the synthesis of purines. One of the three genes, PvPRAT3, is highly expressed in nodules and protein amount and enzymatic activity in these tissues correlate with nitrogen fixation activity. Inhibition of PvPRAT3 gene expression by RNAi-silencing and subsequent metabolomic analysis of the transformed roots shows that PvPRAT3 is essential for the synthesis of ureides in P. vulgaris nodules. © 2016 John Wiley & Sons Ltd.

  7. Purification, cDNA Cloning, and Developmental Expression of the Nodule-Specific Uricase from Phaseolus vulgaris L. 1

    Science.gov (United States)

    Sánchez, Federico; Campos, Francisco; Padilla, Jaime; Bonneville, Jean-Marc; Enríquez, Consuelo; Caput, Daniel

    1987-01-01

    Nodule-specific uricase (uricase II) from Phaseolus vulgaris L. was purified to homogeneity by chromatographic methods. Purification data indicated that uricase II is approximately 2% of the total soluble protein from mature nodules. Specific antiserum was raised and used to determine the developmental expression and for immunoselection of polysomes. Uricase II was antigenically detected early in nodule development, 2 to 3 days before nitrogen fixation. Uricase-encoding cDNA clones were isolated by hybridizing a nodule-specific pUC9 cDNA library with labeled mRNA from immunoselected polysomes and a 35,000 molecular weight uricase II-encoding cDNA from soybean. An homologous clone (pNF-UR07) was used to assess the expression pattern of the specific transcript during development. Northern-blot analysis indicated that uricase II mRNA is exclusively expressed in nodule tissue. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:16665575

  8. Colorado Better Buildings Project. Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Strife, Susie [Boulder County, Boulder, CO (United States); Yancey, Lea [Boulder County, Boulder, CO (United States)

    2013-12-30

    The Colorado Better Buildings project intended to bring new and existing energy efficiency model programs to market with regional collaboration and funding partnerships. The goals for Boulder County and its program partners were to advance energy efficiency investments, stimulate economic growth in Colorado and advance the state’s energy independence. Collectively, three counties set out to complete 9,025 energy efficiency upgrades in 2.5 years and they succeeded in doing so. Energy efficiency upgrades have been completed in more than 11,000 homes and businesses in these communities. Boulder County and its partners received a $25 million BetterBuildings grant from the U.S. Department of Energy under the American Recovery and Reinvestment Act in the summer of 2010. This was also known as the Energy Efficiency and Conservation Block Grants program. With this funding, Boulder County, the City and County of Denver, and Garfield County set out to design programs for the residential and commercial sectors to overcome key barriers in the energy upgrade process. Since January 2011, these communities have paired homeowners and business owners with an Energy Advisor – an expert to help move from assessment to upgrade with minimal hassle. Pairing this step-by-step assistance with financing incentives has effectively addressed many key barriers, resulting in energy efficiency improvements and happy customers. An expert energy advisor guides the building owner through every step of the process, coordinating the energy assessment, interpreting results for a customized action plan, providing a list of contractors, and finding and applying for all available rebates and low-interest loans. In addition to the expert advising and financial incentives, the programs also included elements of social marketing, technical assistance, workforce development and contractor trainings, project monitoring and verification, and a cloud-based customer data system to coordinate among field

  9. Puente Río Colorado - Costa Rica

    Directory of Open Access Journals (Sweden)

    Kulka, F.

    1973-03-01

    Full Text Available The Colorado River bridge is located in a 95 m deep canyon, with a 122 m span. To choose the type of bridge, it has been endeavoured to use the largest possible number of national building materials which, together with the difficulty of reaching the site, meant that a series of classical solutions had to be rejected. That of an arch bridge was adopted, with a reversed support on prestressed cables, on which the road passes. The system is based on the hanging bridge principle, but with the rolling track resting on the cables, instead of hanging from them. There is a first cover, made up of prefabricated components, on the cables, which strengthens the bridge's stability. This cover supports three portal-columns, the pillars of the final roadway. The cables were prestressed from the heads of the two sloping pillars. The two side spans were designed with prefabricated T girders.El puente Río Colorado está situado en un cañón de 95 m de profundidad, salvando una luz de 122 m. Para la elección del tipo de puente se ha procurado emplear el mayor número posible de materiales de construcción nacionales, lo que, unido a la dificultad de acceso a la obra, hizo que se rechazaran una serie de soluciones clásicas. Se adoptó la de un puente-arco con un soporte invertido sobre cables pretensados, encima del cual descansa la calzada. El sistema está basado en los principios del puente colgante, pero apoyando el camino de rodadura en los cables, en lugar de colgarlo de ellos. Sobre los cables existe una primera cubierta, integrada por elementos prefabricados, que refuerza la estabilidad del puente. Esta cubierta soporta tres pórticos-columna, pilares de la calzada definitiva. El pretensado de los cables se realizó desde las cabezas de dos pilares inclinados. Los dos vanos laterales se proyectaron con vigas en T prefabricadas.

  10. Colorado River basin sensitivity to disturbance impacts

    Science.gov (United States)

    Bennett, K. E.; Urrego-Blanco, J. R.; Jonko, A. K.; Vano, J. A.; Newman, A. J.; Bohn, T. J.; Middleton, R. S.

    2017-12-01

    The Colorado River basin is an important river for the food-energy-water nexus in the United States and is projected to change under future scenarios of increased CO2emissions and warming. Streamflow estimates to consider climate impacts occurring as a result of this warming are often provided using modeling tools which rely on uncertain inputs—to fully understand impacts on streamflow sensitivity analysis can help determine how models respond under changing disturbances such as climate and vegetation. In this study, we conduct a global sensitivity analysis with a space-filling Latin Hypercube sampling of the model parameter space and statistical emulation of the Variable Infiltration Capacity (VIC) hydrologic model to relate changes in runoff, evapotranspiration, snow water equivalent and soil moisture to model parameters in VIC. Additionally, we examine sensitivities of basin-wide model simulations using an approach that incorporates changes in temperature, precipitation and vegetation to consider impact responses for snow-dominated headwater catchments, low elevation arid basins, and for the upper and lower river basins. We find that for the Colorado River basin, snow-dominated regions are more sensitive to uncertainties. New parameter sensitivities identified include runoff/evapotranspiration sensitivity to albedo, while changes in snow water equivalent are sensitive to canopy fraction and Leaf Area Index (LAI). Basin-wide streamflow sensitivities to precipitation, temperature and vegetation are variable seasonally and also between sub-basins; with the largest sensitivities for smaller, snow-driven headwater systems where forests are dense. For a major headwater basin, a 1ºC of warming equaled a 30% loss of forest cover, while a 10% precipitation loss equaled a 90% forest cover decline. Scenarios utilizing multiple disturbances led to unexpected results where changes could either magnify or diminish extremes, such as low and peak flows and streamflow timing

  11. Approaches to local climate action in Colorado

    Science.gov (United States)

    Huang, Y. D.

    2011-12-01

    Though climate change is a global problem, the impacts are felt on the local scale; it follows that the solutions must come at the local level. Fortunately, many cities and municipalities are implementing climate mitigation (or climate action) policies and programs. However, they face many procedural and institutional barriers to their efforts, such of lack of expertise or data, limited human and financial resources, and lack of community engagement (Krause 2011). To address the first obstacle, thirteen in-depth case studies were done of successful model practices ("best practices") of climate action programs carried out by various cities, counties, and organizations in Colorado, and one outside Colorado, and developed into "how-to guides" for other municipalities to use. Research was conducted by reading documents (e.g. annual reports, community guides, city websites), email correspondence with program managers and city officials, and via phone interviews. The information gathered was then compiled into a series of reports containing a narrative description of the initiative; an overview of the plan elements (target audience and goals); implementation strategies and any indicators of success to date (e.g. GHG emissions reductions, cost savings); and the adoption or approval process, as well as community engagement efforts and marketing or messaging strategies. The types of programs covered were energy action plans, energy efficiency programs, renewable energy programs, and transportation and land use programs. Between the thirteen case studies, there was a range of approaches to implementing local climate action programs, examined along two dimensions: focus on climate change (whether it was direct/explicit or indirect/implicit) and extent of government authority. This benchmarking exercise affirmed the conventional wisdom propounded by Pitt (2010), that peer pressure (that is, the presence of neighboring jurisdictions with climate initiatives), the level of

  12. Elementary particle physics and high energy phenomena. [Dept. of Physics, Univ. of Colorado, Boulder, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Barker, A.R.; Cumalat, J.P.; De Alwis, S.P.; DeGrand, T.A.; Ford, W.T.; Mahanthappa, K.T.; Nauenberg, U.; Rankin, P.; Smith, J.G.

    1992-06-01

    Experimental and theoretical high-energy physics programs at the University of Colorado are reported. Areas of concentration include the following: study of the properties of the Z[sup 0] with the SLD detector; fixed-target K-decay experiments; the R D program for the muon system: the SDC detector; high-energy photoproduction of states containing heavy quarks; electron--positron physics with the CLEO II detector at CESR; lattice QCD; and spin models and dynamically triangulated random surfaces. 24 figs., 2 tabs., 117 refs.

  13. Effect of a combination of Phaseolus vulgaris L. extract and acarbose on postprandial glucose level after cooked rice intake in healthy volunteers

    Directory of Open Access Journals (Sweden)

    Robby Zulkarnain

    2009-03-01

    Full Text Available Aim This study was aimed to measure the effects of combination Phaseolus vulgaris extract and acarbose compared to acarbose alone on postprandial glucose concentration in healthy volunteers after cooked rice intake.Methods Blood sample were obtained at several time points up to three hours after cooked rice intake. The parameter for postprandial glucose concentration is the area under the curve (AUC of glucose concentration vs.time for three hours after cooked rice intake.Results After taking this combination, postprandial glucose concentration was reduced by 21.6%, while the reduction by acarbose alone was 22.9%.Conclusions The reduction of postprandial glucose concentration after administration of this combination was not significantly different compared to that after administration of acarbose alone. (Med J Indones 2009; 18: 25-30Keywords: Phaseolus vulgaris extract, acarbose, postprandial glucose concentration

  14. Preliminary Site Characterization Report, Rulsion Site, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    This report is a summary of environmental information gathered during a review of the documents pertaining to Project Rulison and interviews with personnel who worked on the project. Project Rulison was part of Operation Plowshare (a program designed to explore peaceful uses for nuclear devices). The project consisted of detonating a 43-kiloton nuclear device on September 10, 1969, in western Colorado to stimulate natural gas production. Following the detonation, a reentry well was drilled and several gas production tests were conducted. The reentry well was shut-in after the last gas production test and was held in standby condition until the general cleanup was undertaken in 1972. A final cleanup was conducted after the emplacement and testing wells were plugged in 1976. However, some surface radiologic contamination resulted from decontamination of the drilling equipment and fallout from the gas flaring during drilling operations. With the exception of the drilling effluent pond, all surface contamination at the Rulison Site was removed during the cleanup operations. All mudpits and other excavations were backfilled, and both upper and lower drilling pads were leveled and dressed. This report provides information regarding known or suspected areas of contamination, previous cleanup activities, analytical results, a review of the regulatory status, the site`s physical environment, and future recommendations for Project Ruhson. Based on this research, several potential areas of contamination have been identified. These include the drilling effluent pond and mudpits used during drilling operations. In addition, contamination could migrate in the gas horizon.

  15. National Uranium Resource Evaluation: Durango Quadrangle, Colorado

    International Nuclear Information System (INIS)

    Theis, N.J.; Madson, M.E.; Rosenlund, G.C.; Reinhart, W.R.; Gardner, H.A.

    1981-06-01

    The Durango Quadrangle (2 0 ), Colorado, was evaluated using National Uranium Resource Evaluation criteria to determine environments favorable for uranium deposits. General reconnaissance, geologic and radiometric investigations, was augmented by detailed surface examination and radiometric and geochemical studies in selected areas. Eight areas favorable for uranium deposits were delineated. Favorable geologic environments include roscoelite-type vanadium-uranium deposits in the Placerville and Barlow Creek-Hermosa Creek districts, sandstone uranium deposits along Hermosa Creek, and vein uranium deposits in the Precambrian rocks of the Needle Mountains area and in the Paleozoic rocks of the Tuckerville and Piedra River Canyon areas. The major portions of the San Juan volcanic field, the San Juan Basin, and the San Luis Basin within the quadrangle were judged unfavorable. Due to lack of information, the roscoelite belt below 1000 ft (300 m), the Eolus Granite below 0.5 mi (0.8 km), and the Lake City caldera are unevaluated. The Precambrian Y melasyenite of Ute Creek and the Animas Formation within the Southern Ute Indian Reservation are unevaluated due to lack of access

  16. Determination of the minimal inhibitory concentration of Ammonium glufosinate in organogenic calli of Phaseolus vulgaris cv. `CIAP 7247F'

    OpenAIRE

    Idalmis Bermúdez-Caraballoso; Raúl Collado; Lourdes R. García; Novisel Veitía; Amanda Martirena; Damaris Torres; Carlos Romero; Gert Angenon

    2012-01-01

    An efficient selection system is necessary for distinguishing transformed cells of the untransformed tissue. This study aimed to determine the minimum inhibitory concentration of the herbicide Ammonium glufosinate in organogenic calli of Phaseolus vulgaris cv. CIAP 7247F, to use it as a selective agent in the process of genetic transformation. Fragments (4-5 mm) of proliferated calli after second subculture were used as explant. Callus proliferation medium with different concentrations of Amm...

  17. Good Days on the Trail, 1938-1942: Film Footage of the Rocky Mountains, Colorado

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This film documents student hiking trips conducted by the University of Colorado at Boulder in the Rocky Mountains, Colorado, USA during the summers of 1938-1942....

  18. Cost-Effectiveness Analysis of the Residential Provisions of the 2015 IECC for Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Mendon, Vrushali V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhao, Mingjie [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Taylor, Zachary T. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Poehlman, Eric A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-02-15

    The 2015 IECC provides cost-effective savings for residential buildings in Colorado. Moving to the 2015 IECC from the 2009 IECC base code is cost-effective for residential buildings in all climate zones in Colorado.

  19. EVALUATING THE ACCUMULATION TREND OF L-DOPA IN DARK-GERMINATED SEEDS AND SUSPENSION CULTURES OF Phaseolus vulgaris L. BY AN EFFICIENT UV-SPECTROPHOTOMETRIC METHOD

    Directory of Open Access Journals (Sweden)

    Samira Rahmani-Nezhad

    Full Text Available Seed germination and plant cell cultures provide an alternative mean for producing secondary metabolites. The present study is an attempt to evaluate the effect of seed dark germination and some elicitors and precursors on the production of L-DOPA in Phaseolus vulgaris L. Callus cultured on Murashige and Skoog medium supplemented with various concentrations of different plant growth regulators. L-DOPA produced was quantified by UV-spectrophotometric method. In this study, a user-friendly, quick, and economical UV-spectrophotometric method was described to determine L-DOPA content in extracts from 33 biotypes of Phaseolus vulgaris L. The method is based on the nitrosation of L-DOPA to form a yellow solution and then formation of a red solution by adding base which is measurable at 470 nm. According to our statistical studies, this method showed high efficiency and selectivity for quantitative determination of L-DOPA in herbal extracts from dried plant seeds, dark-germinated seeds and callus cultures. L-DOPA content in dark-germinated seeds and suspension cultures increased significantly to approximately several-fold compared to the control. The implication from this study is that elicitor treatment and precursor feeding of Phaseolus vulgaris L. can significantly improve the parkinson’s relevant L-DOPA content.

  20. Systematic Review and Meta-Analysis of a Proprietary Alpha-Amylase Inhibitor from White Bean (Phaseolus vulgaris L.) on Weight and Fat Loss in Humans.

    Science.gov (United States)

    Udani, Jay; Tan, Ollie; Molina, Jhanna

    2018-04-20

    The aim of this meta-analysis was to examine the evidence for the effectiveness of a proprietary alpha-amylase inhibitor from white bean ( Phaseolus vulgaris L.) supplementation interventions in humans on modification of body weight and fat mass. A systematic literature search was performed using three databases: PubMed, the Cochrane collaboration, and Google Scholar. In addition, the manufacturer was contacted for internal unpublished data, and finally, the reference section of relevant original research and review papers were mined for additional studies. Eleven studies were selected for the meta-analysis of weight loss (a total of 573 subjects), and three studies for the meta-analysis of body fat reduction (a total of 110 subjects), as they fulfilled the inclusion criteria. Phaseolus vulgaris supplementation showed an average effect on weight loss difference of −1.08 kg (95% CI (confidence interval), −0.42 kg to −1.16 kg, p < 0.00001), and the average effect on body fat reduction was 3.26 kg (95% CI, −2.35 kg to −4.163 kg, p = 0.02). This meta-analysis found statistically significant effects of Phaseolus vulgaris supplementation on body weight and body fat.

  1. Systematic Review and Meta-Analysis of a Proprietary Alpha-Amylase Inhibitor from White Bean (Phaseolus vulgaris L. on Weight and Fat Loss in Humans

    Directory of Open Access Journals (Sweden)

    Jay Udani

    2018-04-01

    Full Text Available The aim of this meta-analysis was to examine the evidence for the effectiveness of a proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris L. supplementation interventions in humans on modification of body weight and fat mass. A systematic literature search was performed using three databases: PubMed, the Cochrane collaboration, and Google Scholar. In addition, the manufacturer was contacted for internal unpublished data, and finally, the reference section of relevant original research and review papers were mined for additional studies. Eleven studies were selected for the meta-analysis of weight loss (a total of 573 subjects, and three studies for the meta-analysis of body fat reduction (a total of 110 subjects, as they fulfilled the inclusion criteria. Phaseolus vulgaris supplementation showed an average effect on weight loss difference of −1.08 kg (95% CI (confidence interval, −0.42 kg to −1.16 kg, p < 0.00001, and the average effect on body fat reduction was 3.26 kg (95% CI, −2.35 kg to −4.163 kg, p = 0.02. This meta-analysis found statistically significant effects of Phaseolus vulgaris supplementation on body weight and body fat.

  2. Colorado Disciplinary Practices, 2008-2010: Disciplinary Actions, Student Behaviors, Race, and Gender

    Science.gov (United States)

    Pfleger, Ryan; Wiley, Kathryn

    2012-01-01

    The Colorado legislature has recently taken school discipline policies under review, pursuant to SB 11-133. To inform the discussion in Colorado as well as a national discussion about discipline, this report presents an analysis of the most complete set of Colorado discipline data. It adds to and reinforces existing studies, documenting some…

  3. 77 FR 42510 - Notice of Inventory Completion: History Colorado, Denver, CO

    Science.gov (United States)

    2012-07-19

    ... associated funerary objects may contact History Colorado. Disposition of the human remains and associated... human remains and associated funerary objects under the control of History Colorado, Denver, CO. One set... detailed assessment of the human remains and associated funerary objects was made by History Colorado...

  4. 77 FR 51792 - Colorado Interstate Gas Company, L.L.C.; Notice of Application

    Science.gov (United States)

    2012-08-27

    ... Interstate Gas Company, L.L.C.; Notice of Application Take notice that on August 7, 2012, Colorado Interstate Gas Company, L.L.C. (CIG), Post Office Box 1087, Colorado Springs, Colorado 80944, filed in the above captioned docket an application pursuant to section 7(c) of the Natural Gas Act (NGA) for a certificate of...

  5. Latinos in Colorado: A Profile of Culture, Changes, and Challenges. Volume V.

    Science.gov (United States)

    Pappas, Georgia, Ed.; Guajardo, Maria, Ed.

    It is projected that the population of Colorado will increase by 25% between 1990 and 2000. The Latino community will experience a slight increase in the proportion of Colorado's population, and will remain the largest ethnic group over the next 30 years. The chapters in this profile describe the Latino population of Colorado. The following essays…

  6. 30 CFR 906.25 - Approval of Colorado abandoned mine land reclamation plan amendments.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Approval of Colorado abandoned mine land reclamation plan amendments. 906.25 Section 906.25 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND... STATE COLORADO § 906.25 Approval of Colorado abandoned mine land reclamation plan amendments. The...

  7. Land and federal mineral ownership coverage for northwestern Colorado

    Science.gov (United States)

    Biewick, L.H.; Mercier, T.J.; Levitt, Pam; Deikman, Doug; Vlahos, Bob

    1999-01-01

    This Arc/Info coverage contains land status and Federal mineral ownership for approximately 26,800 square miles in northwestern Colorado. The polygon coverage (which is also provided here as a shapefile) contains two attributes of ownership information for each polygon. One attribute indicates where the surface is State owned, privately owned, or, if Federally owned, which Federal agency manages the land surface. The other attribute indicates which minerals, if any, are owned by the Federal govenment. This coverage is based on land status and Federal mineral ownership data compiled by the U.S. Geological Survey (USGS) and three Colorado State Bureau of Land Management (BLM) former district offices at a scale of 1:24,000. This coverage was compiled primarily to serve the USGS National Oil and Gas Resource Assessment Project in the Uinta-Piceance Basin Province and the USGS National Coal Resource Assessment Project in the Colorado Plateau.

  8. Environmental assessment, expanded Ponnequin wind energy project, Weld County, Colorado

    International Nuclear Information System (INIS)

    1999-02-01

    The US Department of Energy (DOE) has considered a proposal from the State of Colorado, Office of Energy Conservation (OEC), for funding construction of the Expanded Ponnequin Wind Project in Weld County, Colorado. OEC plans to enter into a contracting arrangement with Public Service Company of Colorado (PSCo) for the completion of these activities. PSCo, along with its subcontractors and business partners, are jointly developing the Expanded Ponnequin Wind Project. The purpose of this Final Environmental Assessment (EA) is to provide DOE and the public with information on potential environmental impacts associated with the Expanded Ponnequin Wind Energy Project. This EA, and public comments received on it, were used in DOE's deliberations on whether to release funding for the expanded project under the Commercialization Ventures Program

  9. Environmental assessment, expanded Ponnequin wind energy project, Weld County, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-02-01

    The US Department of Energy (DOE) has considered a proposal from the State of Colorado, Office of Energy Conservation (OEC), for funding construction of the Expanded Ponnequin Wind Project in Weld County, Colorado. OEC plans to enter into a contracting arrangement with Public Service Company of Colorado (PSCo) for the completion of these activities. PSCo, along with its subcontractors and business partners, are jointly developing the Expanded Ponnequin Wind Project. The purpose of this Final Environmental Assessment (EA) is to provide DOE and the public with information on potential environmental impacts associated with the Expanded Ponnequin Wind Energy Project. This EA, and public comments received on it, were used in DOE`s deliberations on whether to release funding for the expanded project under the Commercialization Ventures Program.

  10. Uranium deposits: northern Denver Julesburg basin, Colorado

    International Nuclear Information System (INIS)

    Reade, H.L.

    1978-01-01

    The Fox Hills Sandstone and the Laramie Formation (Upper Cretaceous) are the host rocks for uranium deposits in Weld County, northern Denver Julesburg basin, Colorado. The uranium deposits discovered in the Grover and Sand Creek areas occur in well-defined north--south trending channel sandstones of the Laramie Formation whereas the sandstone channel in the upper part of the Fox Hills Sandstone trends east--west. Mineralization was localized where the lithology was favorable for uranium accumulation. Exploration was guided by log interpretation methods similar to those proposed by Bruce Rubin for the Powder River basin, Wyoming, because alteration could not be readily identified in drilling samples. The uranium host rocks consist of medium- to fine-grained carbonaceous, feldspathic fluvial channel sandstones. The uranium deposits consist of simple to stacked roll fronts. Reserve estimates for the deposits are: (1) Grover 1,007,000 lbs with an average grade of 0.14 percent eU 3 O 8 ,2) Sand Creek 154,000 lbs with an average grade of 0.08 percent eU 3 O 8 , and 3) The Pawnee deposit 1,060,000 lbs with an average grade of 0.07 percent eU 3 O 8 . The configuration of the geochemical cells in the Grover and Sand Creek sandstones indicate that uraniferous fluids moved northward whereas in the Pawnee sandstone of the Fox Hills uraniferous fluids moved southward. Precipitation of uranium in the frontal zone probably was caused by downdip migration of oxygcnated groundwater high in uranium content moving through a favorable highly carbonaceous and pyritic host sandstone

  11. The characterization of a new set of EST-derived simple sequence repeat (SSR markers as a resource for the genetic analysis of Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Borba Tereza CO

    2011-05-01

    Full Text Available Abstract Background Over recent years, a growing effort has been made to develop microsatellite markers for the genomic analysis of the common bean (Phaseolus vulgaris to broaden the knowledge of the molecular genetic basis of this species. The availability of large sets of expressed sequence tags (ESTs in public databases has given rise to an expedient approach for the identification of SSRs (Simple Sequence Repeats, specifically EST-derived SSRs. In the present work, a battery of new microsatellite markers was obtained from a search of the Phaseolus vulgaris EST database. The diversity, degree of transferability and polymorphism of these markers were tested. Results From 9,583 valid ESTs, 4,764 had microsatellite motifs, from which 377 were used to design primers, and 302 (80.11% showed good amplification quality. To analyze transferability, a group of 167 SSRs were tested, and the results showed that they were 82% transferable across at least one species. The highest amplification rates were observed between the species from the Phaseolus (63.7%, Vigna (25.9%, Glycine (19.8%, Medicago (10.2%, Dipterix (6% and Arachis (1.8% genera. The average PIC (Polymorphism Information Content varied from 0.53 for genomic SSRs to 0.47 for EST-SSRs, and the average number of alleles per locus was 4 and 3, respectively. Among the 315 newly tested SSRs in the BJ (BAT93 X Jalo EEP558 population, 24% (76 were polymorphic. The integration of these segregant loci into a framework map composed of 123 previously obtained SSR markers yielded a total of 199 segregant loci, of which 182 (91.5% were mapped to 14 linkage groups, resulting in a map length of 1,157 cM. Conclusions A total of 302 newly developed EST-SSR markers, showing good amplification quality, are available for the genetic analysis of Phaseolus vulgaris. These markers showed satisfactory rates of transferability, especially between species that have great economic and genomic values. Their diversity

  12. Variación patogénica y fuentes de resistencia a Colletotrichum lindemuthianum Sacc & magn Scrib., patógeno de la antracnosis del frijol, en Colombia Variación patogénica y fuentes de resistencia a Colletotrichum lindemuthianum (Sacc & Magn Scrib., patógeno de la antracnosis del fríjol, en Colombia

    Directory of Open Access Journals (Sweden)

    Cobo S. Felipe

    1987-03-01

    Full Text Available Pathogenic variation studies with colombian isolates of Colletotrichum lindemuthianum (Sacc & Magn Scrib., the anthracnose bean pathogen, were conducted under laboratory and greenhouse conditions at CIAT, Colombia. In addition, bean germplam with cormercial grain color was evaluated with the objective of identifying resistant varieties and sources of resistance to the existing variation. To this purpose, 17 isolates of C. lindemuthianum collected at the principal Colombian bean zones, were inoculated on 21 American and European differential bean varieties.En los laboratorios e invernaderos del programa de fitopatología de fríjol del CIAT, se estudió la variación patogénica de aislamientos colombianos de Colletotrichum lindemuthianum (Sacc& Magn Scrib. También se evaluó germoplasma de frijol con grano de color comercial con el objetivo de identificar líneas resistentes y fuentes de resistencia a la variación existente. Para este fin, 17 aislamientos de C.lindemuthianum, colectados en las principales zonas frijoleras colombianas se inocularon sobre 21 variedades diferenciales.

  13. Physiological and sanity seed quality of common beans (Phaseolus vulgaris L. from Goias state / Qualidade fisiológica e sanitária de sementes de feijoeiro (Phaseolus vulgaris L. provenientes do estado de Goiás

    Directory of Open Access Journals (Sweden)

    Myrna Hilal Moraes

    2008-08-01

    Full Text Available The common beans (Phaseolus vulgaris L. is a fabacea sufficiently spread out in all domestic territory. However, the quality of its seeds represents one of the main causes of low productivity in the beans farmings in Brazil. The objective of this work was to evaluate physiological and sanitary seed qualities of eleven bean cultivars. The physiological seed quality was evaluated trough standard germination and vigor tests. The sanitary seed quality was evaluated through two tests: blotter test was employed to evaluate fungi incidence and “Koch & Menten” method was employed to observe Sclerotinia sclerotiorum (Lib de Bary occurrence. Xamego, BRS Valente, Bambu and Pérola had the best results of physiological tests. Jalo Precoce, Roxo 90, Corrente and Aporé had no good results of vigor and germination, besides presenting the lowest indices of died seeds. Fusarium sp., Aspergillus spp., Penicillium sp., Phoma sp., Rhizopus sp. and Botrytis sp. were the fungi detected in the sanity tests.O feijoeiro comum (Phaseolus vulgaris L. é uma fabacea bastante difundida em todo território nacional. A baixa qualidade de suas sementes representa uma das principais causas de baixa produtividade nas lavouras de feijão no Brasil. O objetivo deste trabalho foi avaliar a qualidade fisiológica e sanitária de sementes de nove cultivares de feijão provenientes do Estado de Goiás. A qualidade fisiológica das sementes foi avaliada através dos testes de germinação e vigor, e a análise sanitária, através dos métodos de papel de filtro, para verificar a ocorrência de fungos em geral, e do método de Koch e Menten, para a avaliação de Sclerotinia sclerotiorum (Lib de Bary. As cultivares que tiveram os melhores desempenhos nos testes fisiológicos foram Xamego, BRS Radiante, Bambu e Pérola. As cultivares Jalo Precoce, Roxo 90, Corrente e Aporé apresentaram baixos índices de vigor e germinação de plântulas normais, além de apresentarem os maiores

  14. Determinação do coeficiente cultural (Kc do feijoeiro (Phaseolus vulgaris L., em Campos dos Goytacazes, RJ Determination of the crop coefficient (Kc for common bean (Phaseolus vulgaris L. in Campos dos Goytacazes, RJ

    Directory of Open Access Journals (Sweden)

    José C. Mendonça

    2007-10-01

    Full Text Available A evapotranspiração de uma cultura é uma das principais informações exigidas para o manejo de irrigação e para fins de planejamento do uso da água. Dentre as abordagens disponíveis para a estimativa do consumo de água pelas plantas, destaca-se o uso de coeficientes de cultura (Kc associados a estimativas da evapotranspiração de referência (ETo. Buscou-se determinar, aqui, os valores de Kc para as diferentes fases fenológicas do feijoeiro comum (Phaseolus vulgaris L., cultivar em lançamento UENF-47, através da utilização de um lisímetro de pesagem e compará-los com os valores propostos pela FAO 56. Concluiu-se que as equações de ajustamento propostas por Allen et al. (1998 se mostraram eficientes para a correção e ajustamento dos coeficientes culturais obtidos neste experimento e que os coeficientes culturais das fases 3 (Kc méd e 4 (Kc fim sugeridos também por Allen et al. (1998 se ajustaram bem ��s condições de cultivo do feijoeiro cultivado no período de outono/inverno, em Campos dos Goytacazes, RJ.The evapotranspiration of a crop is one of the main information required for proper irrigation management and to develop an efficient water usage plan. Among the methods to estimate the amount of water that is consumed by plants, the use of crop coefficients (Kc, associated with estimates of the reference evapotranspiration (ETo, stands as one of the most promising. This work aimed to deterime the values of Kc for different phenological phases of common bean plants (Phaseolus vulgaris L. of the cultivar UENF-47. Determination of Kc values was performed using a weighing lisimeter and results were compared with values obtained through the FAO 56 standard. Results showed that the adjustment equations proposed by Allen et al. (1998 were adequate for fitting the values of Kc obtained in this experiment. It has been shown that the crop coefficients for phenological phases 3 and 4 proposed by Allen et al. (1998 are adequate

  15. The Colorado Plateau II: biophysical, socioeconomic, and cultural research

    Science.gov (United States)

    Mattson, David J.; van Riper, Charles

    2005-01-01

    The publication of The Colorado Plateau: Cultural, Biological, and Physical Research in 2004 marked a timely summation of current research in the Four Corners states. This new volume, derived from the seventh Biennial Conference on the Colorado Plateau in 2003, complements the previous book by focusing on the integration of science into resource management issues. The 32 chapters range in content from measuring human impacts on cultural resources, through grazing and the wildland-urban interface issues, to parameters of climate change on the Plateau. The book also introduces economic perspectives by considering shifting patterns and regional disparities in the Colorado Plateau economy. A series of chapters on mountain lions explores the human-wildland interface. These chapters deal with the entire spectrum of challenges associated with managing this large mammal species in Arizona and on the Colorado Plateau, conveying a wealth of timely information of interest to wildlife managers and enthusiasts. Another provocative set of chapters on biophysical resources explores the management of forest restoration, from the micro scale all the way up to large-scale GIS analyses of ponderosa pine ecosystems on the Colorado Plateau. Given recent concerns for forest health in the wake of fires, severe drought, and bark-beetle infestation, these chapters will prove enlightening for forest service, park service, and land management professionals at both the federal and state level, as well as general readers interested in how forest management practices will ultimately affect their recreation activities. With broad coverage that touches on topics as diverse as movement patterns of rattlesnakes, calculating watersheds, and rescuing looted rockshelters, this volume stands as a compendium of cutting-edge research on the Colorado Plateau that offers a wealth of insights for many scholars.

  16. Utilisation des mutations induites pour l'étude de l'embryogenèse chez le haricot Phaseolus vulgaris L. et deux plantes modèles Arabidopsis thaliana (L. Heynh. et Zea mays L.

    Directory of Open Access Journals (Sweden)

    Silué, S.

    2011-01-01

    Full Text Available Use of induced mutations in embryogenesis study in bean Phaseolus vulgaris L. and two model plants, Arabidopsis thaliana (L. Heynh. and Zea mays L.. Breeding of common bean, Phaseolus vulgaris L., through interspecific hybridizations with the species Phaseolus coccineus L. and Phaseolus polyanthus Greenm. as female parents leads to the abortion of immature embryos. Identification of genes required for embryo development could partly explain the abortion of hybrid embryos; induced mutations could thus be an alternative to identify key genes involved in Phaseolus embryogenesis. This paper is a review which shows a few examples of the use of induced mutations in the identification of essential genes for embryogenesis in two model plants, Arabidopsis thaliana (L. Heyhn. for dicots and Zea mays L. for monocots. In these two species, embryo development mutants have been isolated using insertional mutagenesis and chemical mutagenesis with Ethyl Methane Sulfonate (EMS. Arabidopsis embryo mutants are affected in apical-basal axis polarity, radial pattern and in post-embryonic stages. Some Arabidopsis embryo mutants are defected in auxin signalisation. In maize, defective kernel (dek mutants are affected in the embryo and the endosperm, while in embryo specific (emb mutants, only the embryo is affected. In common bean, plants deficient in seed development were isolated using EMS mutagenesis. Embryos inside the seeds fail to growth at different stages of development and show abnormalities mainly in the suspensor and the cotyledons.

  17. Groundwater quality in the Colorado River basins, California

    Science.gov (United States)

    Dawson, Barbara J. Milby; Belitz, Kenneth

    2012-01-01

    Groundwater provides more than 40 percent of California’s drinking water. To protect this vital resource, the State of California created the Groundwater Ambient Monitoring and Assessment (GAMA) Program. The Priority Basin Project of the GAMA Program provides a comprehensive assessment of the State’s groundwater quality and increases public access to groundwater-quality information. Four groundwater basins along the Colorado River make up one of the study areas being evaluated. The Colorado River study area is approximately 884 square miles (2,290 square kilometers) and includes the Needles, Palo Verde Mesa, Palo Verde Valley, and Yuma groundwater basins (California Department of Water Resources, 2003). The Colorado River study area has an arid climate and is part of the Sonoran Desert. Average annual rainfall is about 3 inches (8 centimeters). Land use in the study area is approximately 47 percent (%) natural (mostly shrubland), 47% agricultural, and 6% urban. The primary crops are pasture and hay. The largest urban area is the city of Blythe (2010 population of 21,000). Groundwater in these basins is used for public and domestic water supply and for irrigation. The main water-bearing units are gravel, sand, silt, and clay deposited by the Colorado River or derived from surrounding mountains. The primary aquifers in the Colorado River study area are defined as those parts of the aquifers corresponding to the perforated intervals of wells listed in the California Department of Public Health database. Public-supply wells in the Colorado River basins are completed to depths between 230 and 460 feet (70 to 140 meters), consist of solid casing from the land surface to a depth of 130 of 390 feet (39 to 119 meters), and are screened or perforated below the solid casing. The main source of recharge to the groundwater systems in the Needles, Palo Verde Mesa, and Palo Verde Valley basins is the Colorado River; in the Yuma basin, the main source of recharge is from

  18. Small Wind Turbine Applications: Current Practice in Colorado

    International Nuclear Information System (INIS)

    Green, Jim

    1999-01-01

    Numerous small wind turbines are being used by homeowners in Colorado. Some of these installations are quite recent while others date back to the federal tax-credit era of the early 1980s. Through visits with small wind turbine owners in Colorado, I have developed case studies of six small wind energy applications focusing on the wind turbine technology, wind turbine siting, the power systems and electric loads, regulatory issues, and motivations about wind energy. These case studies offer a glimpse into the current state-of-the-art of small-scale wind energy and provide some insight into issues affecting development of a wider market

  19. ISOENZYMATIC POLYMORPHISM AND ACTIVITY OF PEROXIDASES OF COMMON BEAN (Phaseolus vulgaris L. UNDER SALINE STRESS

    Directory of Open Access Journals (Sweden)

    F. BROETTO

    1997-09-01

    crude extract of the different bean cultivars analysed showed different reations to salt concentration in the cultivation procedures as well as a high increasing of peroxidase activity in cv. IAC and JALO.Uma das utilizações da técnica de cultura de tecidos para o melhoramento vegetal é a identificação de linhas de células que apresentem tolerância ao estresse salino. Para se estudar os mecanismos bioquímicos envolvidos na expressão genética da tolerância a salinidade, calos oriundos de eixos embrionários de quatro cultivares de feijão (Phaseolus vulgaris L.; cultivares IAC - carioca, IAPAR 14, JALO-EEP 558, BAT - 93, foram cultivados em meio sólido Murashige & Skoog (1962, suplementado com NaCl nas concentrações de 0, 20, 40, 60 e 80 mM. Após 14 dias de incubação, os calos foram coletados e analisados quanto aos padrões isoenzimáticos e de atividade das peroxidases. Os cultivares BAT e IAPAR apresentaram duas zonas de atividade em comum na região anódica e apenas uma zona enzimática específica a cada um deles (migração mais rápida.Possivelmente as duas zonas anódicas intermediárias sejam produtos do mesmo loco enzimático, porém com alelos diferentes, consequentemente diferentes mobilidades eletroforéticas. O cv. JALO apresentou duas zonas anódicas de atividade em comum com os cultivares IAC e IAPAR com uma zona anódica exclusiva de migração mais lenta, a qual apresentou atividade mais intensa de todos os cultivares analisados. Este cultivar revelou ainda uma zona catódica provavelmente dimérica e heterozigota nos indivíduos de todos os tratamentos aplicados. Provavelmente, esta é a mesma zona que ocorre em homozigose com fixação do alelo lento para os indivíduos de todos os tratamentos efetuados nos cultivares BAT e IAPAR. O cv. IAC apresentou duas bandas anódicas em comum com os cv. IAPAR e JALO. Apresentou também a banda anódica mais rápida em comum com o cv. IAPAR e uma banda anódica exclusiva de migração mais

  20. The bio-positive effects of diagnostic doses of X-rays on growth of phaseolus-vulgaris plant

    Energy Technology Data Exchange (ETDEWEB)

    Mortazavi, S.M.J.; Mehdipour, L.A.; Behnejad, B.B. [Rafsanjan Univ. of Medical Sciences (Iran, Islamic Republic of)

    2006-07-01

    Objective: Plants absorb radioactive elements from phosphate fertilizers, and also from naturally occurring radiation in the soil, air and water. It has long been known that low doses of ionizing radiation evoke stimulatory effects in a wide variety of living organisms. However, as far as we know, there is no published report on the bio-positive effects of diagnostic doses of X-rays on plant growth. The aim of this study was to evaluate the bio-effects of low doses of diagnostic X-rays on growth rate of Phaseolus vulgaris (Pinto) plants. Materials and Methods: Before cultivation, Phaseolus vulgaris (Pinto) seeds were soaked in tap water for 2 days followed by another 2 days of covering under a wet cloth. Four hundred newly cultivated seeds were randomly divided into two groups of 200 plants each. In this experiment, two seeds were cultivated in each dish (100 dishes for irradiation group and 100 for sham-irradiation group). Fifteen days after starting cultivation, newly grown plants were irradiated with X-rays. Plants were exposed to a single dose of X-ray (80 kVp, 80 mAs) for 6 days. On day 29, plants were pulled out from the ' soil. Length of plant stem, length of root, number of leaves and plant weight were measured. Results: The stem length in irradiated and sham-irradiated plants was 296.5{+-}13.57 and 223.96{+-}15.02 mm respectively. This difference was statistically significant (P<0.001). Although the number of leaves in irradiated plants was higher than that of sham-irradiated plants (7.05{+-}0.18 and 6.74{+-}0.19 respectively), the difference was not statistically significant. The stem diameter in irradiated and sham-irradiated plants were 3.52{+-}0.12 and 3.35{+-}0.09 mm respectively, but the difference again was not statistically significant (P<0.00 1). Plant weight in irradiated samples was less than that of non-irradiated plants but it was not statistically significant. Conclusions: The overall results indicate that diagnostic doses of X-rays can

  1. 75 FR 3333 - Irish Potatoes Grown in Colorado; Modification of the Handling Regulation for Area No. 2

    Science.gov (United States)

    2010-01-21

    ... FR] Irish Potatoes Grown in Colorado; Modification of the Handling Regulation for Area No. 2 AGENCY... requirement under the Colorado potato marketing order, Area No. 2. The marketing order regulates the handling of Irish potatoes grown in Colorado, and is administered locally by the Colorado Potato...

  2. Assessment of surface-water quantity and quality, Eagle River watershed, Colorado, 1947-2007

    Science.gov (United States)

    Williams, Cory A.; Moore, Jennifer L.; Richards, Rodney J.

    2011-01-01

    From the early mining days to the current tourism-based economy, the Eagle River watershed (ERW) in central Colorado has undergone a sequence of land-use changes that has affected the hydrology, habitat, and water quality of the area. In 2000, the USGS, in cooperation with the Colorado River Water Conservation District, Eagle County, Eagle River Water and Sanitation District, Upper Eagle Regional Water Authority, Colorado Department of Transportation, City of Aurora, Town of Eagle, Town of Gypsum, Town of Minturn, Town of Vail, Vail Resorts, City of Colorado Springs, Colorado Springs Utilities, and Denver Water, initiated a retrospective analysis of surface-water quantity and quality in the ERW.

  3. Beyond Colorado's Front Range - A new look at Laramide basin subsidence, sedimentation, and deformation in north-central Colorado

    Science.gov (United States)

    Cole, James C.; Trexler, James H.; Cashman, Patricia H.; Miller, Ian M.; Shroba, Ralph R.; Cosca, Michael A.; Workman, Jeremiah B.

    2010-01-01

    This field trip highlights recent research into the Laramide uplift, erosion, and sedimentation on the western side of the northern Colorado Front Range. The Laramide history of the North Park?Middle Park basin (designated the Colorado Headwaters Basin in this paper) is distinctly different from that of the Denver basin on the eastern flank of the range. The Denver basin stratigraphy records the transition from Late Cretaceous marine shale to recessional shoreline sandstones to continental, fluvial, marsh, and coal mires environments, followed by orogenic sediments that span the K-T boundary. Upper Cretaceous and Paleogene strata in the Denver basin consist of two mega-fan complexes that are separated by a 9 million-year interval of erosion/non-deposition between about 63 and 54 Ma. In contrast, the marine shale unit on the western flank of the Front Range was deeply eroded over most of the area of the Colorado Headwaters Basin (approximately one km removed) prior to any orogenic sediment accumulation. New 40Ar-39Ar ages indicate the oldest sediments on the western flank of the Front Range were as young as about 61 Ma. They comprise the Windy Gap Volcanic Member of the Middle Park Formation, which consists of coarse, immature volcanic conglomerates derived from nearby alkalic-mafic volcanic edifices that were forming at about 65?61 Ma. Clasts of Proterozoic granite, pegmatite, and gneiss (eroded from the uplifted core of the Front Range) seem to arrive in the Colorado Headwaters Basin at different times in different places, but they become dominant in arkosic sandstones and conglomerates about one km above the base of the Colorado Headwaters Basin section. Paleocurrent trends suggest the southern end of the Colorado Headwaters Basin was structurally closed because all fluvial deposits show a northward component of transport. Lacustrine depositional environments are indicated by various sedimentological features in several sections within the >3 km of sediment

  4. Ozone impact on vegetation: phenolic metabolism modification and oxidative alteration of Rubisco in Phaseolus vulgaris L; Impact de l'ozone sur le vegetal: modification du metabolisme phenolique et alteration de la Rubisco chez Phaseolus vulgaris L.

    Energy Technology Data Exchange (ETDEWEB)

    Kanoun, M.

    2002-04-15

    In order to characterize and quantify, in semi-natural situation, the incidence of atmospheric pollution on some physiological and metabolic functions in plants, the aim of our work was to identify sub-cellular impact markers, in bean (Phaseolus vulgaris L.), able to characterize a chronic and realistic ozone pollution climate. Two criteria were chosen: the foliar phenolic metabolism and the Rubisco, the key enzyme of photosynthesis. Using Open Top Chambers system, we demonstrated that, according to concentration, exposure kinetic and leaf type, ozone could induce amount variations of some constitutive soluble phenolic and the synthesis of new phenolic (iso-flavonoids). In some cases, these disturbances were observed jointly with foliar injuries and/or biomass reduction. Concurrently, this chronic and moderate ozone exposure could also induce carbonyl formation in amino acid residues constitutive of Rubisco small subunit (Rubisco-SSU) and a reduction in the amount of the native Rubisco. The amount of a constitutive kaempferol glucuronide and the ozone-induced oxidative alteration of Rubisco-SSU were selected and tested for the construction of dose-response relationships. Whatever the marker, the linear model was able to describe the relation. For the phenolic response, several exposure indexes were tested. According to their mode of calculation, these exposure forms emphasize more or less the contribution of high ozone concentrations. If, for Rubisco oxidation, the use of the exposure index AOT40 seems relevant, in the case of the phenolic marker, the choice of the right index is leaf type dependant. (author)

  5. Standardization of a rearing procedure of Tetranychus urticae Koch (Acari: Tetranychidae) on bean (Phaseolus vulgaris): plant age and harvest time; Padronizacao da criacao de Tetranychus urticae Koch (Acari: Tetranychidae) em feijoeiro (Phaseolus vulgaris): idade da planta e tempo de colheita

    Energy Technology Data Exchange (ETDEWEB)

    Bustos, Alexander; Cantor, Fernando; Cure, Jose R; Rodriguez, Daniel [Universidade Militar Nueva Granada, Bogota (Colombia). Facutad de Ciencias. Programa de Biologia Aplicada], e-mail: fernando.cantor@unimilitar.edu.co

    2009-09-15

    A rearing technique was standardized to produce Tetranychus urticae Koch on Phaseolus vulgaris (ICA Cerinza variety) as a prey of the predatory mite Phytoseiulus persimilis Athias-Henriot. Two assays were conducted to assess the following variables: the most suitable plant age for mite infestation, and the best time to harvest the mites and re infest the plants. In the first experiment, four, five, six, and seven-week-old plants of P. vulgaris were infested with six T. urticae per foliole. The lower plant stratum exhibited the largest number of mites regardless of plant age. However, four-week old plants had the larger average number of individuals. In the second experiment four-week-old plants were infested with 0.5 female mite/cm{sup 2} of leaf. The number of individuals per instar of T. urticae was recorded weekly. The highest mite production occurred between four and five weeks after infestation, indicating this to be the most suitable for mite harvesting and for plant reinfestation. (author)

  6. Biologia e tabela de vida de Tetranychus desertorum (Acari: Tetranychidae sobre folhas de feijão (Phaseolus vulgaris Biology and life table of Tetranychus desertorum (Acari: Tetranychidae on leaves of kidney bean (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Edgar Rivero

    2009-03-01

    Full Text Available Biological aspects and life table of the red spider mite, Tetranychus desertorum Banks, 1900, were studied on leaf discs of kidney bean (Phaseolus vulgaris Linnaeus cultivar "Tacarigua" under laboratory conditions (28 ± 2ºC, 70 ± 10% R.H. and 12:12h. Our results showed that total developmental time was 6.8 days for females, with partial duration of immature stages corresponding to 3.8, 1.4, 1.0 and 0.7 for egg, larva, protonymph and deutonymph, respectively. Preoviposition, oviposition and postoviposition periods were 1.1, 8.4 and 1.3 days, respectively; and the higher mean fecundity (6.93 eggs/female/day was observed on day 4. Female mean longevity was 10 days. The life table parameters recorded were: net reproduction rate (Ro = 41.10 individuals; generation time (T = 11.15 days; intrinsic natural growth (r m = 0.144 individuals/female/day, and finite natural increase rate (λ = 1.155 individuals/female. Our findings could be a basis for further studies devoted to determine damage and control strategies for T. desertorum on kidney bean crops.

  7. Salinity Trends in the Upper Colorado River Basin Upstream From the Grand Valley Salinity Control Unit, Colorado, 1986-2003

    Science.gov (United States)

    Leib, Kenneth J.; Bauch, Nancy J.

    2008-01-01

    In 1974, the Colorado River Basin Salinity Control Act was passed into law. This law was enacted to address concerns regarding the salinity content of the Colorado River. The law authorized various construction projects in selected areas or 'units' of the Colorado River Basin intended to reduce the salinity load in the Colorado River. One such area was the Grand Valley Salinity Control Unit in western Colorado. The U. S. Geological Survey has done extensive studies and research in the Grand Valley Salinity Control Unit that provide information to aid the U.S. Bureau of Reclamation and the Natural Resources Conservation Service in determining where salinity-control work may provide the best results, and to what extent salinity-control work was effective in reducing salinity concentrations and loads in the Colorado River. Previous studies have indicated that salinity concentrations and loads have been decreasing downstream from the Grand Valley Salinity Control Unit, and that the decreases are likely the result of salinity control work in these areas. Several of these reports; however, also document decreasing salinity loads upstream from the Grand Valley Salinity Control Unit. This finding was important because only a small amount of salinity-control work was being done in areas upstream from the Grand Valley Salinity Control Unit at the time the findings were reported (late 1990?s). As a result of those previous findings, the U.S. Bureau of Reclamation entered into a cooperative agreement with the U.S. Geological Survey to investigate salinity trends in selected areas bracketing the Grand Valley Salinity Control Unit and regions upstream from the Grand Valley Salinity Control Unit. The results of the study indicate that salinity loads were decreasing upstream from the Grand Valley Salinity Control Unit from 1986 through 2003, but the rates of decrease have slowed during the last 10 years. The average rate of decrease in salinity load upstream from the Grand Valley

  8. Colorado Plateau magmatism and uplift by warming of heterogeneous lithosphere.

    Science.gov (United States)

    Roy, Mousumi; Jordan, Thomas H; Pederson, Joel

    2009-06-18

    The forces that drove rock uplift of the low-relief, high-elevation, tectonically stable Colorado Plateau are the subject of long-standing debate. While the adjacent Basin and Range province and Rio Grande rift province underwent Cenozoic shortening followed by extension, the plateau experienced approximately 2 km of rock uplift without significant internal deformation. Here we propose that warming of the thicker, more iron-depleted Colorado Plateau lithosphere over 35-40 Myr following mid-Cenozoic removal of the Farallon plate from beneath North America is the primary mechanism driving rock uplift. In our model, conductive re-equilibration not only explains the rock uplift of the plateau, but also provides a robust geodynamic interpretation of observed contrasts between the Colorado Plateau margins and the plateau interior. In particular, the model matches the encroachment of Cenozoic magmatism from the margins towards the plateau interior at rates of 3-6 km Myr(-1) and is consistent with lower seismic velocities and more negative Bouguer gravity at the margins than in the plateau interior. We suggest that warming of heterogeneous lithosphere is a powerful mechanism for driving epeirogenic rock uplift of the Colorado Plateau and may be of general importance in plate-interior settings.

  9. Being PREPaREd for Crisis in Northern Colorado

    Science.gov (United States)

    Hancock, Kathy; Malvey, Michelle; Rastatter, Dennis

    2010-01-01

    The Thompson School District recognized after the Columbine incident in the spring of 1999 that it was lacking an adequate plan for crisis response. Colorado legislation led to a mandate for having a crisis response plan so the district purchased a "canned" crisis response plan that served the needs of response in a very immediate but…

  10. Examining the Spatial Distribution of Marijuana Establishments in Colorado

    Science.gov (United States)

    Kerski, Joseph

    2018-01-01

    In this 22-question activity, high school students investigate the spatial distribution of marijuana stores in Colorado using an interactive web map containing stores, centers, highways, population, and other data at several scales. After completing this lesson, students will know and be able to: (1) Use interactive maps, layers, and tools in…

  11. Agricultural water conservation programs in the lower Colorado River Authority

    International Nuclear Information System (INIS)

    Kabir, J.

    1993-01-01

    Rice irrigation is the largest user of water within the area served by the Lower Colorado River Authority (LCRA), accounting for approximately 75 percent of total annual surface and ground water demands. In an average year, about 30 percent of surface water supplied to rice irrigation is satisfied with water released from the storage in the Highland Lakes located at the upstream reaches of the Lower Colorado River and its tributaries. During a severe drought, the demand for stored water could be as much as 70 percent of annual rice irrigation demand. LCRA owns and operates two irrigation canal systems which together supply water to irrigate 60,000 acres of rice each year. These irrigation systems are the Lakeside and Gulf Coast Irrigation Divisions. The Lakeside system is located in Colorado and Wharton Counties and the Gulf Coast system is located in Wharton and Matagorda Counties. In the 1987 and 1989, the Lower Colorado River Authority Board of Directors authorized implementation and funding for Canal Rehabilitation Project and Irrigation Water Measurement Project respectively. These two projects are key initiatives to agricultural water conservation goals established in the LCRA Water Management Plan and Water Conservation Policy. In addition LCRA participated actively in agricultural water conservation research projects and technology transfer activities

  12. Radiation balance in a deep Colorado valley: ASCOT 84

    International Nuclear Information System (INIS)

    Whiteman, C.D.; Fritschen, L.J.; Simpson, J.R.; Orgill, M.M.

    1984-12-01

    Five surface energy budget stations were installed at four sites in a deep, narrow valley in western Colorado as part of the Atmospheric Studies in Complex Terrain (ASCOT) Study. Radiation balance data are presented from these stations for the clear day September 29, 1984. 3 references, 3 figures, 3 tables

  13. Colorado State University (CSU) accelerator and FEL facility

    NARCIS (Netherlands)

    Milton, S.; Biedron, S.; Harris, J.; Martinez, J.; D'Audney, A.; Edelen, J.; Einstein, J.; Hall, C.; Horovitz, K.; Morin, A.; Sipahi, N.; Sipahi, T.; Williams, J.; Carrico, C.; Van Der Slot, P. J M

    2014-01-01

    The Colorado State University (CSU) Accelerator Facility will include a 6-MeV L-Band (1.3 GHz) electron linear accelerator (linac) with a free-electron laser (FEL) system capable of producing Terahertz (THz) radiation, a laser laboratory, a microwave test laboratory, and a magnetic test laboratory.

  14. Transgenic resistance of eggplants to the Colorado potato beetle

    NARCIS (Netherlands)

    Arpaia, S.

    1999-01-01

    The subject of this thesis is the use of transgenic plant resistance as a method to control the Colorado potato beetle, Leptinotarsa decemlineata Say in eggplant. The gene conferring resistance is coding for a Cry3B toxin and it is a synthetic version of a wild-type

  15. Digital Learning Compass: Distance Education State Almanac 2017. Colorado

    Science.gov (United States)

    Seaman, Julia E.; Seaman, Jeff

    2017-01-01

    This brief report uses data collected under the U.S. Department of Education's National Center for Educational Statistics (NCES) Integrated Postsecondary Education Data System (IPEDS) Fall Enrollment survey to highlight distance education data in the state of Colorado. The sample for this analysis is comprised of all active, degree-granting…

  16. Discovery of cryptic Armillaria solidipes genotypes within the Colorado Plateau

    Science.gov (United States)

    J. W. Hanna; N. B. Klopfenstein; M. -S. Kim; S. M. Ashiglar; A. L. Ross-Davis; G. I. McDonald

    2012-01-01

    Armillaria solidipes (= A. ostoyae) is a root-disease pathogen that causes severe losses in growth and productivity of forest trees throughout the Northern Hemisphere. This species is genetically diverse with variable disease activities across different regions of the world. In North America, A. solidipes in the Colorado Plateau exists in drier habitats and causes more...

  17. Construction of calibration pads facility, Walker Field, Grand Junction, Colorado

    International Nuclear Information System (INIS)

    Ward, D.L.

    1978-08-01

    A gamma-ray spectrometer facility was completed at Walker Field Airport, Grand Junction, Colorado, in November 1976. This report describes spectrometers and their calibration, the construction of the spectrometer facility, the radioelement concentrations, procedures for using the facilites, and environmental considerations

  18. The Colorado Gambling Boom: An Experiment in Rural Community Development.

    Science.gov (United States)

    Stokowski, Patricia A.

    1992-01-01

    Three small Colorado towns that faced a declining economy as the mining resource ran out used gambling-based tourism as a strategy for community development. Although economic benefits to the towns have far exceeded expectations, negative social, environmental, and political changes, such as crime alcoholism, traffic problems, and conflicts…

  19. Colorado's Alternative School Calendar Program and the Four Day Week.

    Science.gov (United States)

    Brubacher, Roy G.; Stiverson, C. L.

    Taking advantage of legislation permitting modified school calendars, the four-day work week has been implemented by 23 small, rural Colorado school districts representing 5,200 children. Thirteen districts implemented the four-day program in the 1980-81 school year. Ten additional districts applied as first year pilot programs in the 1981-82…

  20. Colorado Handbook for State-Funded Student Assistance Programs. Revised.

    Science.gov (United States)

    Colorado Commission on Higher Education, Denver.

    Policies and procedures established by the Colorado Commission on Higher Education for the use of state-funded student assistance are presented. Annual budget ranges, sample forms, and instructions are included. In addition to providing definitions and general policy information, the guidelines cover fund application and allocation; accounting,…

  1. Radon from coal ash in Colorado. Final report

    International Nuclear Information System (INIS)

    Morse, J.G.

    1981-01-01

    An attempt was made to develop a radiation profile of Colorado, based on available measured radon data. Data reported indicate that the release of radon to the atmosphere during uranium milling and mining is not a significant health hazard or air pollutant, when compared with radon in soils. 1 figure, 2 tables

  2. Molecular cloning and characterization of a gene encoding the proline transporter protein in common bean(Phaseolus vulgaris L.)

    Institute of Scientific and Technical Information of China (English)

    Jibao; Chen; Jing; Wu; Yunfeng; Lu; Yuannan; Cao; Hui; Zeng; Zhaoyuan; Zhang; Lanfen; Wang; Shumin; Wang

    2016-01-01

    As a typical compatible solute, proline is accumulated in plants under environmental stresses. Proline transporter(Pro T) plays an important role in proline distribution between plant organs. Using a candidate gene approach, we cloned a c DNA sequence for Pro T from common bean(Phaseolus vulgaris L.) and designated the gene Pv Pro T. The deduced amino acid sequence of Pv Pro T showed high similarity to Bet/Pro T proteins from other leguminous plants, and the highest similarity was observed with mothbean(Vigna aconitifolia L.) Vu Pro T.Relative quantification of the m RNA level of Pv Pro T using real-time PCR analysis showed that the Pv Pro T transcript level was higher in leaves than in stems and roots of common bean plants subjected to drought and salt stress. Under 20%(w/w) PEG-6000 treatment,drought-resistant plants expressed a higher level of Pv Pro T transcripts than droughtsensitive plants. Although heterologous expression of Pv Pro T in the Escherichia coli mutant mkh13 showed that Pv Pro T exhibited uptake activities for proline and betaine, no betaine content was detected in the common bean. These findings suggest that Pv Pro T plays an important role in the transportation of proline in common bean plants exposed to drought and salt stress.

  3. Efficiency of Trichome-Based Plant Defense in Phaseolus vulgaris Depends on Insect Behavior, Plant Ontogeny, and Structure

    Directory of Open Access Journals (Sweden)

    Zhenlong Xing

    2017-11-01

    Full Text Available Plant trichomes often function as physical barriers in preventing arthropod feeding and oviposition. Even though insects are frequently reported being entrapped and killed by trichome traps, the actual trapping behavior has not yet been described in detail. Capture experiments showed that capture efficiency during the plant's vegetative stage was considerably higher than in the fruiting and cotyledon stages. The ventral surface of the leaf was more effective in trapping flies than other parts of the plant. Capture-events monitoring showed that the mouthparts, legs, and ovipositor of Liriomyza trifolii adults are the body parts involved in entrapment by surface trichomes on Phaseolus vulgaris plants, and subsequently, deter their ability to feed, walk, and oviposit. Of the three main body parts normally affected, mouthparts was found to be the body part most susceptible to the trichomes. Entrapments were most often caused by landing, followed by puncturing or feeding, and occasionally by walking or fighting. Using scanning electron microscopy (SEM and optical microscopy, we determined the susceptible positions of each body part and found that the flies were all trapped by hooked trichomes. This study revealed the process by which leafminer flies are entrapped by surface trichomes of the host plant and evaluated the capture efficiency. The results will contribute to our understanding of physical defenses against herbivores.

  4. Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.).

    Science.gov (United States)

    Pascale, Raffaella; Bianco, Giuliana; Cataldi, Tommaso R I; Kopplin, Philippe-Schmitt; Bosco, Federica; Vignola, Lisiana; Uhl, Jenny; Lucio, Marianna; Milella, Luigi

    2018-03-01

    The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC 50 =1.1±0.1μg/ml and IC 50 =19.3±1.1μg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity. Copyright © 2017. Published by Elsevier Ltd.

  5. Consumer acceptance and aroma characterization of navy bean (Phaseolus vulgaris) powders prepared by extrusion and conventional processing methods.

    Science.gov (United States)

    Szczygiel, Edward J; Harte, Janice B; Strasburg, Gale M; Cho, Sungeun

    2017-09-01

    Food products produced with bean ingredients are gaining in popularity among consumers due to the reported health benefits. Navy bean (Phaseolus vulgaris) powder produced through extrusion can be considered as a resource-efficient alternative to conventional methods, which often involve high water inputs. Therefore, navy bean powders produced with extrusion and conventional methods were assessed for the impact of processing on consumer liking in end-use products and odor-active compounds. Consumer acceptance results reveal significant differences in flavor, texture and overall acceptance scores of several products produced with navy bean powder. Crackers produced with extruded navy bean powder received higher hedonic flavor ratings than those produced with commercial navy bean powder (P < 0.001). GC-O data showed that the commercial powder produced through conventional processing had much greater contents of several aliphatic aldehydes commonly formed via lipid oxidation, such as hexanal, octanal and nonanal with descriptors of 'grassy', 'nutty', 'fruity', 'dusty', and 'cleaner', compared to the extruded powder. Extrusion processed navy bean powders were preferred over commercial powders for certain navy bean powder applications. This is best explained by substantial differences in aroma profiles of the two powders that may have been caused by lipid oxidation. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Physical and chemical characterization of composite flour from canna flour (Canna edulis) and lima bean flour (Phaseolus lunatus)

    Science.gov (United States)

    Praseptiangga, Danar; Tryas, Anisha Ayuning; Affandi, Dian Rachmawanti; Atmaka, Windi; Ariyantoro, Achmad Ridwan; Minardi, Slamet

    2018-02-01

    The diversity of Indonesian local food sources has potential to be developed for supporting food security based development of local food diversification. Canna tubers (Canna edulis) and lima beans (Phaseolus lunatus) are two local commodities in Indonesia which under is utilization and has limited assessment to its characteristics. This study aimed at determining the best formula of composite flour based on physical and chemical properties of composite flour produced. There were three formulas, F1 for 85% of canna flour and 15%of lima beans flour, F2 for 70% of canna flour and 30% of lima beans flour and F3 for 55% of canna flour and 45% of lima beans flour. Physical and chemical analyses were conducted and completely randomized design was used. De Garmo analysis was then used to determine the highest effectiveness index from the three formulas developed in this study and F3 demonstrated the highest effectiveness index (0.545) among three formulas evaluated. Thus, formula (F3) was selected as the best composite of the flour developed from canna flour and lima beans flour.

  7. The Class II trehalose 6-phosphate synthase gene PvTPS9 modulates trehalose metabolism in Phaseolus vulgaris nodules.

    Directory of Open Access Journals (Sweden)

    Aarón Barraza

    2016-11-01

    Full Text Available Legumes form symbioses with rhizobia, producing nitrogen-fixing nodules on the roots of the plant host. The network of plant signaling pathways affecting carbon metabolism may determine the final number of nodules. The trehalose biosynthetic pathway regulates carbon metabolism and plays a fundamental role in plant growth and development, as well as in plant-microbe interactions. The expression of genes for trehalose synthesis during nodule development suggests that this metabolite may play a role in legume-rhizobia symbiosis. In this work, PvTPS9, which encodes a Class II trehalose-6-phosphate synthase (TPS of common bean (Phaseolus vulgaris, was silenced by RNA interference in transgenic nodules. The silencing of PvTPS9 in root nodules resulted in a reduction of 85% (± 1% of its transcript, which correlated with a 30% decrease in trehalose contents of transgenic nodules and in untransformed leaves. Composite transgenic plants with PvTPS9 silenced in the roots showed no changes in nodule number and nitrogen fixation, but a severe reduction in plant biomass and altered transcript profiles of all Class II TPS genes. Our data suggest that PvTPS9 plays a key role in modulating trehalose metabolism in the symbiotic nodule and, therefore, in the whole plant.

  8. Physiological and molecular analysis of the interaction between aluminium toxicity and drought stress in common bean (Phaseolus vulgaris)

    Science.gov (United States)

    Yang, Zhong-Bao; Eticha, Dejene; Albacete, Alfonso; Rao, Idupulapati Madhusudana; Roitsch, Thomas; Horst, Walter Johannes

    2012-01-01

    Aluminium (Al) toxicity and drought are two major factors limiting common bean (Phaseolus vulgaris) production in the tropics. Short-term effects of Al toxicity and drought stress on root growth in acid, Al-toxic soil were studied, with special emphasis on Al–drought interaction in the root apex. Root elongation was inhibited by both Al and drought. Combined stresses resulted in a more severe inhibition of root elongation than either stress alone. This result was different from the alleviation of Al toxicity by osmotic stress (–0.60 MPa polyethylene glycol) in hydroponics. However, drought reduced the impact of Al on the root tip, as indicated by the reduction of Al-induced callose formation and MATE expression. Combined Al and drought stress enhanced up-regulation of ACCO expression and synthesis of zeatin riboside, reduced drought-enhanced abscisic acid (ABA) concentration, and expression of NCED involved in ABA biosynthesis and the transcription factors bZIP and MYB, thus affecting the regulation of ABA-dependent genes (SUS, PvLEA18, KS-DHN, and LTP) in root tips. The results provide circumstantial evidence that in soil, drought alleviates Al injury, but Al renders the root apex more drought-sensitive, particularly by impacting the gene regulatory network involved in ABA signal transduction and cross-talk with other phytohormones necessary for maintaining root growth under drought. PMID:22371077

  9. Standardization of a rearing procedure of Tetranychus urticae Koch (Acari: Tetranychidae) on bean (Phaseolus vulgaris): plant age and harvest time

    International Nuclear Information System (INIS)

    Bustos, Alexander; Cantor, Fernando; Cure, Jose R; Rodriguez, Daniel

    2009-01-01

    A rearing technique was standardized to produce Tetranychus urticae Koch on Phaseolus vulgaris (ICA Cerinza variety) as a prey of the predatory mite Phytoseiulus persimilis Athias-Henriot. Two assays were conducted to assess the following variables: the most suitable plant age for mite infestation, and the best time to harvest the mites and re infest the plants. In the first experiment, four, five, six, and seven-week-old plants of P. vulgaris were infested with six T. urticae per foliole. The lower plant stratum exhibited the largest number of mites regardless of plant age. However, four-week old plants had the larger average number of individuals. In the second experiment four-week-old plants were infested with 0.5 female mite/cm 2 of leaf. The number of individuals per instar of T. urticae was recorded weekly. The highest mite production occurred between four and five weeks after infestation, indicating this to be the most suitable for mite harvesting and for plant reinfestation. (author)

  10. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Minhee, E-mail: heelee@pknu.ac.kr [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of); Yang, Minjune [Department of Environmental Geosciences, Pukyong National University, 599-1 Daeyondong, Namgu, Busan 608-737 (Korea, Republic of)

    2010-01-15

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24 h by using sunflower and the residual uranium concentration of the treated water was lower than 30 {mu}g/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25 mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  11. Biosynthesis, isolation and characterization of {sup 57}Fe-enriched Phaseolus vulgaris ferritin after heterologous expression in Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Hoppler, Matthias [ETH Zurich, Laboratory of Human Nutrition, Zurich (Switzerland); Meile, Leo [ETH Zurich, Laboratory of Food Biotechnology, Zurich (Switzerland); Walczyk, Thomas [National University of Singapore, Department of Chemistry and Department of Biochemistry, Singapore (Singapore)

    2008-01-15

    Ferritin is the major iron storage protein in the biosphere. Iron stores of an organism are commonly assessed by measuring the concentration of the protein shell of the molecule in fluids and tissues. The amount of ferritin-bound iron, the more desirable information, still remains inaccessible owing to the lack of suitable techniques. Iron saturation of ferritin is highly variable, with a maximum capacity of 4,500 iron atoms per molecule. This study describes the direct isotopic labeling of a complex metalloprotein in vivo by biosynthesis, in order to measure ferritin-bound iron by isotope dilution mass spectrometry. [{sup 57}Fe]ferritin was produced by cloning and overexpressing the Phaseolus vulgaris ferritin gene pfe in Escherichia coli in the presence of {sup 57}FeCl{sub 2}. Recombinant ferritin was purified in a fully assembled form and contained approximately 1,000 iron atoms per molecule at an isotopic enrichment of more than 95% {sup 57}Fe. We did not find any evidence of species conversion of the isotopic label for at least 5 months of storage at -20 C. Transfer efficiency of enriched iron into [{sup 57}Fe]ferritin of 20% was sufficient to be economically feasible. Negligible amounts of non-ferritin-bound iron in the purified [{sup 57}Fe]ferritin solution allows for use of this spike for quantification of ferritin-bound iron by isotope dilution mass spectrometry. (orig.)

  12. Resistance or tolerance to the golden mosaic virus of bean plant (Phaseolus vulgaris L.), obtained by mutation induction

    International Nuclear Information System (INIS)

    Tulmann Neto, A.

    1979-09-01

    Experiments were carried out with the objective of selecting, evaluation and using induced mutants of Phaseolus vulgaris L. resistant or tolerant to golden mosaic - a virus disease of beans. Seeds from three bean cultivars were treated with gamma-ray or the chemical mutagen ethyl methanesulphonate (EMS). After golden mosaic inoculation of 50,000 M 2 seedlings, in a insectary, screening was made and a tolerant mutant (TMD-1) was selected. Evaluation of TMD-1 was carried out by comparing it with the parent cultivar Carioca, indicating that, although showing lower productivity than the original material, (what prevented it from being used directly on a commercial basis), it maintained the same reaction to rust, bacterial blight, and common mosaic. Studies on the genetic basis of the mutation were also done. The possibility of using this mutant in a plant breeding programme aimed at obtaining resistance to golden mosaic was demonstrated in crosses between TMD-1 and two cultivars, to which transference of tolerance was possible. (Author) [pt

  13. Steady-state room temperature fluorescence and CO/sub 2/ assimilation rates in intact leaves. [Phaseolus vulgaris; Xanthium strumarium

    Energy Technology Data Exchange (ETDEWEB)

    Sharkey, T D

    1985-01-01

    Steady-state room temperature variable fluorescence from leaves was measured as a function of CO/sub 2/ pressure in Xanthium strumarium L. and Phaseolus vulgaris L. Measurements were made in a range of light intensities, at normal and low O/sub 2/ partial pressure and over a range of temperatures. At low CO/sub 2/ pressure fluorescence increased with increasing CO/sub 2/. At higher CO/sub 2/ pressure fluorescence usually decreased with increasing CO/sub 2/ but occasionally increased slightly. The transition CO/sub 2/ pressure between the responses could be changed by changing light, O/sub 2/ pressure, or temperature. This breakpoint in the fluorescence-CO/sub 2/ curve was a reliable indicator of the transition between ribulose 1,5-bisphosphate (RuBP) saturated assimilation and RuBP regeneration limited assimilation. The fluorescence signal was not a reliable indicator of O/sub 2/-insensitive assimilation in these C/sub 3/ species. 21 references, 8 figures.

  14. Canopy reflectance indices and its relationship with yield in common bean plants (Phaseolus vulgaris L.) with phosphorus supply

    International Nuclear Information System (INIS)

    Rodriguez, M.G.; Escalante-Estrada, J.A.; Gonzalez, M.T.R.; Reynolds, M.P.

    2006-01-01

    Common bean plants (Phaseolus vulgaris L.) were grown under three phosphorous levels (0,100 & 200 kg ha-1) and under rain fed conditions with the objective to examine the association between vegetative indices (NDVI, normalized difference vegetation index; and GNDVI, green normalized difference vegetation index) and intercepted radiation, leaf area index, biomass and yield during the growing season. The maximum intercepted radiation, leaf area index (LAI) and biomass were reached during the pod filling stage {80 days after sowing (DAS)}, and the P treatment of 200 kg ha-1 showed the highest values. The high intercepted radiation was derived from an increase in LAI inducing a major biomass accumulation. Near to physiological maturity LAI decreased as a result of leaf abscission. NDVI and GNDVI were higher with P supply than without P at anthesis and pod filling stage (50 - 80 DAS). Near to physiological maturity, NDVI and GNDVI decreased in all the treatments . When the maximum intercepted radiation, LAI, and biomass production were reached during anthesis and pod filling stage, NDVI and GNDVI also had the highest values. The association between the vegetative indices and seed yield during the pod filling stage showed a linear relationship by the P supply. The relationship between GNDVI and seed yield was higher (r2 = 0.77) than the relationship between NDVI and seed yield (r2 = 0.61)

  15. The characteristics of pyrophosphate: D-fructose-6-phosphate 1-phosphotransferases from Sansevieria trifasciata leaves and Phaseolus coccineus stems.

    Science.gov (United States)

    Kowalczyk, S

    1987-01-01

    Three different molecular forms of pyrophosphate-dependent phosphofructokinase have been isolated: one from Sansevieria trifasciata leaves and two from Phaseolus coccineus stems. The form isolated from S. trifasciata has the molecular weight of about 115,000. The apparent molecular weights for the two forms from mung bean were approximately 220,000 and 450,000. All three forms have the same pH optima, an absolute requirement for Mg2+ ions both in the forward and reverse reaction, but differ in their sensitivity toward fructose 2,6-bisphosphate. Kinetic properties of the partially purified enzymes have been investigated in the presence and absence of fructose 2,6-bisphosphate. Pyrophosphate-dependent phosphofructokinase from S. trifasciata exhibited hyperbolic kinetics with all substrates tested. The saturation curves of the enzyme (form A) from mung bean for pyrophosphate, fructose 6-phosphate and fructose 1,6-bisphosphate were sigmoidal in the absence of fructose 2,6-bisphosphate. In the presence of fructose 2,6-bisphosphate these kinetics became hyperbolic.

  16. Desempeño del Phaseolus vulgaris bajo riego parcial cultivado en un sistema de respuesta hidrogravitrópica

    Directory of Open Access Journals (Sweden)

    Emil Cristhian Vega Ponce

    2017-01-01

    Full Text Available In the experimental area of the UNALM (Lima - Perú was evaluated on partial root - zone drying irrigation treatments (RPR300 and RPR500, ml and full irrigation (RC300 a nd RC500, ml control, the impact of xylem potential ( x and stomatal conductance (gs in common bean plants ( Phaseolus vulgaris L. cultivated in systems (pots of hydrogravitropic response. A randomized complete block design with 12 plants/pots per trea tments was used in three replicates. To control the irrigation application and manage to maintain the x nonlethal conditions (< - 15 bars, a water - soil retention curve was generated. The values of gs before irrigation (between 217.18 and 268.67 mm m - 2 s - 1 showed that only RPR500 plants were maintained under optimal water conditions, despite low levels of x (between - 9.92 and - 7.33 bar; situation that could be attributed to the ability of the roots to balance those moments when half of these structures we re inside soil with low humidity, while the opposite half was favorable soil water level.

  17. myo-Inositol synthesis from [1-3H]glucose in Phaseolus vulgaris L. during early stages of germination

    International Nuclear Information System (INIS)

    Sasaki, K.; Taylor, I.E.P.

    1986-01-01

    Radiolabeled D-[1- 3 H]glucose was fed by imbibition under sterile conditions to bean (Phaseolus vulgaris L.) seeds. After 72 and 96 hours of feeding, the 3 H was located in uronic acid and pentose residues as well as hexose residues of cell wall polysaccharides in growing hypocotyl and root. Free myo-inositol present in cotyledons, hypocotyl, and root also contained 3 H, showing that de novo synthesis of myo-inositol from [1- 3 H]glucose did occur during the first 72 hours of germination. More than 90% of the labeled, free myo-inositol was present in the cotyledons. The 3 H percentage in trifluoroacetic acid-soluble arabinaose residues of cell wall polysaccharides from 72-hour-old bean hypocotyls was only half of their mole percentage. On the other hand, 3 H percentages in hexose residues were higher than their mole percentages. The results suggest that myo-inositol is synthesized from reserve sugars during the very early stages of germination, and that the newly synthesized myo-inositol, as well as that stored in cotyledons, can be used for the construction of new hypocotyl and root cell wall polysaccharides after conversion into uronic acids and pentoses via the myo-inositol oxidation pathway

  18. Angiotensin-converting enzyme-inhibitory activity in protein hydrolysates from normal and anthracnose disease-damaged Phaseolus vulgaris seeds.

    Science.gov (United States)

    Hernández-Álvarez, Alan Javier; Carrasco-Castilla, Janet; Dávila-Ortiz, Gloria; Alaiz, Manuel; Girón-Calle, Julio; Vioque-Peña, Javier; Jacinto-Hernández, Carmen; Jiménez-Martínez, Cristian

    2013-03-15

    Bean seeds are an inexpensive source of protein. Anthracnose disease caused by the fungus Colletotrichum lindemuthianum results in serious losses in common bean (Phaseolus vulgaris L.) crops worldwide, affecting any above-ground plant part, and protein dysfunction, inducing the synthesis of proteins that allow plants to improve their stress tolerance. The aim of this study was to evaluate the use of beans damaged by anthracnose disease as a source of peptides with angiotensin-converting enzyme (ACE-I)-inhibitory activity. Protein concentrates from beans spoiled by anthracnose disease and from regular beans as controls were prepared by alkaline extraction and precipitation at isolelectric pH and hydrolysed using Alcalase 2.4 L. The hydrolysates from spoiled beans had ACE-I-inhibitory activity (IC(50) 0.0191 mg protein mL(-1)) and were very similar to those from control beans in terms of ACE-I inhibition, peptide electrophoretic profile and kinetics of hydrolysis. Thus preparation of hydrolysates using beans affected by anthracnose disease would allow for revalorisation of this otherwise wasted product. The present results suggest the use of spoiled bean seeds, e.g. anthracnose-damaged beans, as an alternative for the isolation of ACE-I-inhibitory peptides to be further introduced as active ingredients in functional foods. © 2012 Society of Chemical Industry.

  19. Rhizofiltration using sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) to remediate uranium contaminated groundwater

    International Nuclear Information System (INIS)

    Lee, Minhee; Yang, Minjune

    2010-01-01

    The uranium removal efficiencies of rhizofiltration in the remediation of groundwater were investigated in lab-scale experiments. Sunflower (Helianthus annuus L.) and bean (Phaseolus vulgaris L. var. vulgaris) were cultivated and an artificially uranium contaminated solution and three genuine groundwater samples were used in the experiments. More than 80% of the initial uranium in solution and genuine groundwater, respectively, was removed within 24 h by using sunflower and the residual uranium concentration of the treated water was lower than 30 μg/L (USEPA drinking water limit). For bean, the uranium removal efficiency of the rhizofiltration was roughly 60-80%. The maximum uranium removal via rhizofiltration for the two plant cultivars occurred at pH 3-5 of solution and their uranium removal efficiencies exceeded 90%. The lab-scale continuous rhizofiltration clean-up system delivered over 99% uranium removal efficiency, and the results of SEM and EDS analyses indicated that most uranium accumulated in the roots of plants. The present results suggested that the uranium removal capacity of two plants evaluated in the clean-up system was about 25 mg/kg of wet plant mass. Notably, the removal capacity of the root parts only was more than 500 mg/kg.

  20. IMPACT OF LIQUID NITROGEN EXPOSURE ON SELECTED BIOCHEMICAL AND STRUCTURAL PARAMETERS OF HYDRATED Phaseolus vulgaris L. SEEDS.

    Science.gov (United States)

    Cejas, Inaudis; Rivas, Maribel; Nápoles, Lelurlys; Marrero, Pedro; Yabor, Lourdes; Aragón, Carlos; Pérez, Aurora; Engelmann, Florent; Martínez-Montero, Marcos Edel; Lorenzo, José Carlos

    2015-01-01

    It is well known that cryopreserving seeds with high water content is detrimental to survival, but biochemical and structural parameters of cryostored hydrated common bean seeds have not been published. The objective of this work was to study the effect of liquid nitrogen exposure on selected biochemical and structural parameters of hydrated Phaseolus vulgaris seeds. We cryopreserved seeds at various moisture contents and evaluated: germination; electrolyte leakage; fresh seed weight; levels of chlorophyll pigments, malondialdehyde, other aldehydes, phenolics and proteins; thickness of cotyledon epidermis, parenchyma, and starch storage parenchyma; and radicle and plumule lengths. Germination was totally inhibited when seeds were immersed in water for 50 min (moisture content of 38%, FW basis) before cryopreservation. The combined effects of seed water imbibition and cryostorage decreased phenolics (free, cell wall-linked, total), chlorophyll a and protein content. By contrast, electrolyte leakage and levels of chlorophyll b and other aldehydes increased as a result of the combination of these two experimental factors. These were the most significant effects observed during exposure of humid seed to liquid nitrogen. Further studies are still required to clarify the molecular events taking place in plant cells during cryostorage.

  1. Bioremediation model for atrazine contaminated agricultural soils using phytoremediation (using Phaseolus vulgaris L.) and a locally adapted microbial consortium.

    Science.gov (United States)

    Madariaga-Navarrete, Alfredo; Rodríguez-Pastrana, Blanca Rosa; Villagómez-Ibarra, José Roberto; Acevedo-Sandoval, Otilio Arturo; Perry, Gregory; Islas-Pelcastre, Margarita

    2017-06-03

    The objective of the present study was to examine a biological model under greenhouse conditions for the bioremediation of atrazine contaminated soils. The model consisted in a combination of phytoremediation (using Phaseolus vulgaris L.) and rhizopheric bio-augmentation using native Trichoderma sp., and Rhizobium sp. microorganisms that showed no inhibitory growth at 10,000 mg L -1 of herbicide concentration. 33.3 mg of atrazine 50 g -1 of soil of initial concentration was used and an initial inoculation of 1 × 10 9 UFC mL -1 of Rhizobium sp. and 1 × 10 5 conidia mL -1 of Trichoderma sp. were set. Four treatments were arranged: Bean + Trichoderma sp. (B+T); Bean + Rhizobium sp. (BR); Bean + Rhizobium sp. + Trichoderma sp. (B+R+T) and Bean (B). 25.51 mg of atrazine 50 g -1 of soil (76.63%) was removed by the B+T treatment in 40 days (a = 0.050, Tukey). This last indicate that the proposed biological model and methodology developed is useful for atrazine contaminated bioremediation agricultural soils, which can contribute to reduce the effects of agrochemical abuse.

  2. Evidence for the endophytic colonization of Phaseolus vulgaris(common bean roots by the diazotroph Herbaspirillum seropedicae

    Directory of Open Access Journals (Sweden)

    M.A. Schmidt

    2011-03-01

    Full Text Available Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8 CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4 cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6 CFU/g. The identity of the recovered bacteria was confirmed by amplification and sequencing of the 16SrRNA gene. Furthermore, confocal microscopy of P. vulgaris roots inoculated with H. seropedicae RAM4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that H. seropedicae RAM4 is capable of colonizing the roots of the dicotyledon P. vulgaris. Determination of dry weight of common bean inoculated with H. seropedicae SMR1 suggested that this bacterium has a negative effect on the growth of P. vulgaris.

  3. Friend or Foe-Light Availability Determines the Relationship between Mycorrhizal Fungi, Rhizobia and Lima Bean (Phaseolus lunatus L..

    Directory of Open Access Journals (Sweden)

    Daniel J Ballhorn

    Full Text Available Plant associations with root microbes represent some of the most important symbioses on earth. While often critically promoting plant fitness, nitrogen-fixing rhizobia and arbuscular mycorrhizal fungi (AMF also demand significant carbohydrate allocation in exchange for key nutrients. Though plants may often compensate for carbon loss, constraints may arise under light limitation when plants cannot extensively increase photosynthesis. Under such conditions, costs for maintaining symbioses may outweigh benefits, turning mutualist microbes into parasites, resulting in reduced plant growth and reproduction. In natural systems plants commonly grow with different symbionts simultaneously which again may interact with each other. This might add complexity to the responses of such multipartite relationships. We experimented with lima bean (Phaseolus lunatus, which efficiently forms associations with both types of root symbionts. We applied full light and low-light to each of four treatments of microbial inoculation. After an incubation period of 14 weeks, we quantified vegetative aboveground and belowground biomass and number and viability of seeds to determine effects of combined inoculant and light treatment on plant fitness. Under light-limited conditions, vegetative and reproductive traits were inhibited in AMF and rhizobia inoculated lima bean plants relative to controls (un-colonized plants. Strikingly, reductions in seed production were most critical in combined treatments with rhizobia x AMF. Our findings suggest microbial root symbionts create additive costs resulting in decreased plant fitness under light-limited conditions.

  4. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean Genome

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    Aihua Wang

    2012-09-01

    Full Text Available In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (HE and observed heterozygosity (HO levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.

  5. Sonchus oleraceus Residue Improves Nutritive and Health-Promoting Value of Common Bean ( Phaseolus vulgaris L.): A Metabolic Study.

    Science.gov (United States)

    Hassan, Mahmoud O; Saleh, Ahmed M; AbdElgawad, Hamada

    2018-03-07

    This study was conducted to evaluate the use of the phenolic-rich Sonchus oleraceus residue as an environmentally safe approach to induce the nutritive and health-promoting values of common bean ( Phaseolus vulgaris L. cv. Bronco). S. oleraceus shoot residue, at rates of 150 and 300 g m -2 , has improved soil fertility via accumulation of soil macronutrients, organic matter, organic carbon, and total phenolics. The growth and yield of bean were significantly increased. Moreover, chemical composition of the treated seeds was significantly altered, whereas higher levels of total antioxidant capacity, proteins, carbohydrates, and most of the individual phenolic acids, flavonoids, vitamins, essential amino acids, and unsaturated fatty acids were recorded. Interestingly, a concentration dependent effect was also observed, for instance, a lower saturated-to-unsaturated fatty acid ratio was only observed in the case of the lower residue rate. These findings recommend the use of S. oleraceus in organic farming of bean to enhance the health benefits of the produced seeds.

  6. Interaction between cadmium and iron. Accumulation and distribution of metals and changes in growth parameters of Phaseolus vulgaris L. seedlings

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    Anna Siedlecka

    2014-01-01

    Full Text Available The interaction between cadmium, one of the most toxic heavy metals, and iron, an essential plant nutritional element, was investigated in Phaseolus vulgaris L. (cv. Słowianka seedlings. The interaction was externally induced by changing the content of both metals in the nutrient medium. Under iron deficiency conditions (0 and 0.5 of normal dose of this element, the toxic effects of cadmium on plant growth parameters, like fresh and dry weight accumulation, primary leaves area, etc., were generally much more pronounced than under normal iron supply. At normal and excess iron supply (1, 2 and 4 doses cadmium diminished iron accumulation in roots and primary leaves, but on the other hand excess iron decreased cadmium level, preventing plants from extreme toxicity of very high cadmium concentrations in the growth environment. It is to be noted that iron is classified also as a heavy metal, and its excess may become toxic, e.g. decreasing root dry weight or diminishing leaf area, especially at the highest dose. The detoxication role of iron against cadmium, and possibly other toxic metals is, however, limited to concentrations of this element in the nutrient solution which themselves are not toxic for the organism.

  7. Effect of Dehydration Conditions on the Chemical, Physical, and Rehydration Properties of Instant Whole Bean (Phaseolus vulgaris L. var. Azufrado

    Directory of Open Access Journals (Sweden)

    Juan Alberto Resendiz Vazquez

    2015-01-01

    Full Text Available The purpose of this study was to evaluate the effect of dehydration conditions on the chemical, physical, and rehydration properties of instant whole beans (Phaseolus vulgaris L. var. Azufrado using a 22 factorial design (air temperature: 25°C and 30°C, air velocity: 0.5 m/s and 1.0 m/s. To determine the kinetic parameters, the rehydration data were fitted to three models: Peleg’s, First Order, and Sigmoid. The protein, fat, and ash contents of the beans were not significantly affected (P>0.05 by the dehydration conditions. Of the 11 physical properties of the instant whole beans, only water activity and splitting were significantly affected by dehydration conditions (P0.05 between the observed and predicted equilibrium moisture contents of the instant whole beans. Regarding the rehydration kinetics for the instant whole beans, the activation energy values ranged from 23.56 kJ/mol to 30.48 kJ/mol, depending on the dehydration conditions. The dehydration conditions had no significant effect (P>0.05 on the rehydration properties of instant whole beans.

  8. Breeding for culinary and nutritional quality of common bean (Phaseolus vulgaris L. in intercropping systems with maize (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Rodino A.P.

    1999-01-01

    Full Text Available Common bean (Phaseolus vulgaris L. is widely intercropped with maize (Zea mays L. in the North of Spain. Breeding beans for multiple cropping systems is important for the development of a productive and sustainable agriculture, and is mainly oriented to minimize intercrop competition and to stabilize complementarity with maize. Most agricultural research on intercropping to date has focused on the agronomic and overall yield effects of the different species, but characters related with socio-economic and food quality aspects are also important. The effect of intercropping beans with maize on food seed quality traits was studied for thirty-five bush bean varieties under different environments in Galicia (Northwestern Spain. Parameters determining Asturian (Northern Spain white bean commercial and culinary quality have also been evaluated in fifteen accessions. There are significant differences between varieties in the selected cropping systems (sole crop, intercrop with field maize and intercrop with sweet maize for dry and soaked seed weight, coat proportion, crude protein, crude fat and moisture. Different white bean accessions have been chosen according to their culinary quality. Under these environmental conditions it appears that intercropping systems with sweet maize give higher returns than sole cropping system. It is also suggested that the culinary and nutritional quality potential of some white bean accessions could be the base material in a breeding programme the objectives of which are to develop varieties giving seeds with high food quality.

  9. Cellulosimicrobium funkei-like enhances the growth of Phaseolus vulgaris by modulating oxidative damage under Chromium(VI toxicity

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    Chinnannan Karthik

    2016-11-01

    Full Text Available Contamination of agriculture land by heavy metals is a worldwide risk that has sped up noticeably since the beginning of the industrial revolution. Hence, there arise the demands of heavy metal tolerant plant growth promoting bacterial strains for specific metal contaminated agricultural sites restoration. In this study, 36 bacterial isolates were screened out from the rhizospheric soil of Phaseolus vulgaris. Among these, two bacterial strains AR6 and AR8 were selected based on their higher Cr(VI tolerance (1200 and 1100 μg/mL, respectively and the maximum production of plant growth promoting substances. In the molecular characterization study, both the bacterial strains showed 99% homology with Cellulosimicrobium funkei KM032184. In greenhouse experiments, the exposure of Cr(VI to P.vulgaris inhibited the growth and photosynthetic pigments and increased the enzymatic and non-enzymatic antioxidant expressions. However, rhizosphere bacterial inoculations alleviated the negative effect of Cr(VI and enhanced the seed germination rate (89.54%, shoot (74.50%,root length (60%, total biomass (52.53%, chlorophyll a (15.91%, chlorophyll b (17.97%, total chlorophyll (16.58% and carotenoid content (3.59%. Moreover, bacterial inoculations stabilized and modulated the antioxidant system of P. vulgaris by reducing the accumulation of Cr in plant tissues. The present finding shows the Cr(VI tolerance and plant growth promoting properties of the rhizosphere bacterial strains which might make them eligible as biofertilizer of metal-contaminated soils.

  10. Evaluation of some residual bioactivities of microencapsulated Phaseolus lunatus protein fraction with carboxymethylated flamboyant (Delonix regia gum/sodium alginate

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    Mukthar Sandovai-Peraza

    2014-12-01

    Full Text Available Recent studies have shown the beneficial effect of peptides, an unexploited source could be Phaseolus lunatus being an important raw material for those functional products in order to improve their utilization. In addition to improve the beneficial effect of bioactive peptides the microencapsulation could be a way to protect the peptides against the environment to which they are exposed. P. lunatus protein fraction (<10 kDa of weight was encapsulated using a blend of carboxymethylated flamboyant gum (CFG and sodium alginate (SA at different concentrations of CaCl2 and hardening times. After in vitro digestion of microcapsules the residual activity, in the intestinal system, both inhibition of agiotensin-converting enzyme (I-ACE and antioxidant activity obtained were in a range of 0.019-0.136 mg/mL and 570.64-813.54 mM of TEAC respectively. The microencapsulation employed CFG/SA blends could be used controlled delivery of peptide fractions with potential use as a nutraceutical or therapeutic agents.

  11. Functional and conformational properties of phaseolin (Phaseolus vulgris L.) and kidney bean protein isolate: a comparative study.

    Science.gov (United States)

    Yin, Shou-Wei; Tang, Chuan-He; Wen, Qi-Biao; Yang, Xiao-Quan

    2010-03-15

    Kidney bean (Phaseolus vulgris L.) seed is an underutilised plant protein source with good potential to be applied in the food industry. Phaseolin (also named G1 globulin) represents about 50 g kg(-1) of total storage protein in the seed. The aim of the present study was to characterise physicochemical, functional and conformational properties of phaseolin, and to compare these properties with those of kidney bean protein isolate (KPI). Compared with kidney bean protein isolate (KPI), the acid-extracted phaseolin-rich protein product (PRP) had much lower protein recovery of 320 g kg(-1) (dry weight basis) but higher phaseolin purity (over 950 g kg(-1)). PRP contained much lower sulfhydryl (SH) and disulfide bond contents than KPI. Differential scanning calorimetry analyses showed that the phaseolin in PRP was less denatured than in KPI. Thermal analyses in the presence or absence of dithiothreitol, in combination with SH and SS content analyses showed the contributions of SS to the thermal stability of KPI. The analyses of near-UV circular dichroism and intrinsic fluorescence spectra indicated more compacted tertiary conformation of the proteins in PRP than in KPI. PRP exhibited much better protein solubility, emulsifying activity index, and gel-forming ability than KPI. The relatively poor functional properties of KPI may be associated with protein denaturation/unfolding, with subsequent protein aggregation. The results presented here suggest the potential for acid-extracted PRP to be applied in food formulations, in view of its functional properties.

  12. Molecular cloning and characterization of a gene encoding the proline transporter protein in common bean (Phaseolus vulgaris L.

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    Jibao Chen

    2016-10-01

    Full Text Available As a typical compatible solute, proline is accumulated in plants under environmental stresses. Proline transporter (ProT plays an important role in proline distribution between plant organs. Using a candidate gene approach, we cloned a cDNA sequence for ProT from common bean (Phaseolus vulgaris L. and designated the gene PvProT. The deduced amino acid sequence of PvProT showed high similarity to Bet/ProT proteins from other leguminous plants, and the highest similarity was observed with mothbean (Vigna aconitifolia L. VuProT. Relative quantification of the mRNA level of PvProT using real-time PCR analysis showed that the PvProT transcript level was higher in leaves than in stems and roots of common bean plants subjected to drought and salt stress. Under 20% (w/w PEG-6000 treatment, drought-resistant plants expressed a higher level of PvProT transcripts than drought-sensitive plants. Although heterologous expression of PvProT in the Escherichia coli mutant mkh13 showed that PvProT exhibited uptake activities for proline and betaine, no betaine content was detected in the common bean. These findings suggest that PvProT plays an important role in the transportation of proline in common bean plants exposed to drought and salt stress.

  13. Utilization of soil and fertilizer zinc by maize (Zea mays) and moong (Phaseolus aureus Roxb.) from some Indian soils

    International Nuclear Information System (INIS)

    Iyengar, B.R.V.; Deb, D.L.

    1977-01-01

    Utilization of soil and fertilizer zinc by maize (Zea mays) and moong (Phaseolus aureus Roxb.) was studied under greenhouse conditions in ten soils belonging to three important soil groups of India viz. alluvial, red and laterite using two levels of labelled zinc sulphate. The efficiency of utilization of fertilizer zinc was found to be higher at 10 ppm level of application in both the crops as compared with 20 ppm level of applied zinc. The total utilization of fertilizer zinc by both the crops in different soils ranged from 0.47 to 1.34 percent and 0.41 to 0.95 percent at 10 and 20 ppm level of applied zinc, respectively. Application of fertilizer zinc resulted in a decrease in the uptake of soil zinc. The utilization of fertilizer zinc was found to be negatively correlated with soil pH but the interaction of different soil characters seemed to have determined the efficiency of fertilizer use by both the crops. The efficiency of fertilizer zinc utilization was found to be low in moong as compared with maize. (author)

  14. Heat flow in the north-central Colorado Plateau

    International Nuclear Information System (INIS)

    Bodell, J.M.; Chapman, D.S.

    1982-01-01

    We report new heat flow measurements at 25 evenly distributed sites in the north-central Colorado Plateau. Heat flow values computed for these new sites and one previously published site range from 43 to 116 mW m -2 but fall into the following district subsets related to physiographic and tectonic elements within the Plateau: (1) heat flow of 51 mW m -2 (12 sites; s.d. 6) in the San Rafael Swell and Green River Desert which constitute the core of the Colorado Plateau at this latitude, (2) heat flows of 69 mW m -2 (5 sites; s.d. 10) in successive parallel north-south bands approaching the Wasatch Plateau to the west but still 80 km east of the Basin and Range physiographic boundary, (3) heat flow of 64 mW m -2 (5 sites; s.d. 2) along the Salt Anticline trend which strikes northwest in the region of Moab, Utah. Heat flow results for the entire Colorado Plateau have been reexamined in view of our new results, and the overall pattern supports the concept of a low heat flow 'thermal interior' for the plateau surrounded by a periphery some 100 km wide having substantially higher heat flow. Average heat flow in the thermal interior is about 60 mW m -2 compared to 80--90 mW m -2 in the periphery. This regional heat flow pattern supports a model of tertiary lithospheric thinning under the Colorado Plateau whereby the plateau is still in transient thermal response and a 15--20 m.y. lag between uplift and corresponding surface heat flow anomaly is to be expected. The position of the heat flow transition between our interior and peripheral regions in the northwest plateau is roughly consistent with lateral warming and weakening of the Colorado Plateau lithosphere initiated at the Basin and Range boundary some 20 m.y. ago

  15. Economic impact study of the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado state fiscal year 1994

    International Nuclear Information System (INIS)

    1994-11-01

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year 1994. To capture employment information, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Naturita, Gunnison, and Rifle, Colorado. Economic data were requested from each site prime subcontractor, as well as from the Remedial Action Contractor. The most significant benefits associated with the UMTRA Project in Colorado are summarized. This study assesses benefits associated with the Grand Junction, Gunnison, Naturita, and Rifle UMTRA Projects sites for the 1-year period under study. Work at the Naturita site was initiated in April 1994 and involved demolition of buildings at the processing site. Actual start-up of remediation of Naturita is planned to begin in the spring of 1995. Work at the Slick Rock and Maybell sites is expected to begin in 1995. The only current economic benefits associated with these sites are related to UMTRA Project support work

  16. 78 FR 52984 - Stone Age Interiors, Inc.; d/b/a Colorado Springs Marble and Granite Including On-Site Leased...

    Science.gov (United States)

    2013-08-27

    ....; d/b/a Colorado Springs Marble and Granite Including On-Site Leased Workers From Express Employment... Colorado Springs Marble and Granite, Colorado Springs, Colorado (hereafter collectively referred to as..., Inc., d/b/a Colorado Springs Marble and Granite, including on-site leased workers from Express...

  17. Burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (Lycopersicon esculentum) with the ability to effectively nodulate Phaseolus vulgaris.

    Science.gov (United States)

    Martínez-Aguilar, Lourdes; Salazar-Salazar, Corelly; Méndez, Rafael Díaz; Caballero-Mellado, Jesús; Hirsch, Ann M; Vásquez-Murrieta, María Soledad; Estrada-de los Santos, Paulina

    2013-12-01

    During a survey of Burkholderia species with potential use in agrobiotechnology, a group of 12 strains was isolated from the rhizosphere and rhizoplane of tomato plants growing in Mexico (Nepantla, Mexico State). A phylogenetic analysis of 16S rRNA gene sequences showed that the strains are related to Burkholderia kururiensis and Burkholderia mimosarum (97.4 and 97.1 %, respectively). However, they induced effective nitrogen-fixing nodules on roots of Phaseolus vulgaris. Based on polyphasic taxonomy, the group of strains represents a novel species for which the name Burkholderia caballeronis sp. nov. is proposed. The type species is TNe-841(T) (= LMG 26416(T) = CIP 110324(T)).

  18. Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae in transgenic bean plants (Phaseolus vulgaris L., Fabaceae

    Directory of Open Access Journals (Sweden)

    Aragão F.J.L.

    1999-01-01

    Full Text Available Bean (Phaseolus vulgaris, an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methionine-rich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R2 to R5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23% over the values found in untransformed plants.

  19. Efeito da irrigação com água salina em um solo cultivado com o feijoeiro (Phaseolus vulgaris L. Effect of irrigation water salinity in a soilcultivated with french beans (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Márcio José de Santana

    2003-04-01

    Full Text Available Conduziu-se este trabalho com o objetivo de avaliar os efeitos de diferentes concentrações de sal, da água de irrigação, na salinização de um Latossolo Roxo distrófico, onde cultivou-se o feijoeiro (Phaseolus vulgaris L. CV ESAL 686. O experimento foi conduzido em casa-de-vegetação no Departamento de Engenharia da Universidade Federal de Lavras, em Lavras, MG, com o propósito de evitar a interferência das precipitações pluviométricas. Os tratamentos consistiram de cinco níveis de salinidade da água (condutividade elétrica de 0,1; 1,0; 2,5; 4,0 e 5,5 dS m-1 com seis repetições. A condutividade elétrica do extrato saturado do solo foi medida no início do experimento, no final da fase vegetativa e após a colheita. Constatou-se uma diminuição da salinidade do solo para o tratamento 0,1 dS m-1, nas diferentes datas de análise do extrato. Para os demais tratamentos, houve um aumento significativo na salinidade: 116,98%, 195,10%, 565,84% e 955,17% para os níveis 1,0; 2,5; 4,0 e 5,5 dS m-1, respectivamente. Houve uma queda acentuada de produção com níveis crescentes de salinidade do solo. O aumento da salinidade da água promoveu um acréscimo linear na condutividade elétrica do solo e no potencial osmótico.The objective this study was to evaluate the different irrigation water salt concentrations effects in the salinization of a "Dystrophic Dusky Red Latossol", cultivated with (Phaseolus vulgaris L. CV ESAL 686. The experiment was carried out in a greenhouse in the Engineering Department at Federal University of Lavras, of Lavras - MG to avoid the interference of the precipitations. The treatments consisted of five level of water salt concentration (electric conductivity of 0.10; 1.0; 2.5; 4.0 and 5.5 dS m-1 with six replications. The electric conductivity of the soil saturation extract was measured at the beginning of the experiment, at the end of the vegetative phase and after the crop harvest. A decrease of soil

  20. Qualidade da colheita mecanizada de feijão (Phaseolus vulgaris em dois sistemas de preparo do solo Quality of the mechanical harvesting of beans (Phaseolus vulgaris under two tillage systems

    Directory of Open Access Journals (Sweden)

    Rouverson Pereira da Silva

    2013-03-01

    Full Text Available Dentre as etapas de produção do feijoeiro a colheita é uma das mais importantes, porque pode interferir de maneira decisiva na qualidade e no custo de produção. Assim, objetivou-se avaliar a qualidade da operação da colheita mecanizada de feijão (Phaseolus vulgaris, cultivado sob preparo convencional e plantio direto. As variáveis analisadas foram: o nível de ruído emitido, calculado através de um medidor de pressão sonora; o desempenho operacional, sendo monitorado o consumo de combustível, a patinagem dos rodados e a velocidade de deslocamento do conjunto coletados em uma central digital (datalogger; e a operação de colheita quanto à matéria seca e densidade de palhada, e as perdas na colheita. A velocidade e os consumos horário e operacional apresentaram distribuição normal dos dados, enquanto que o nível de ruído apresentou distribuição assimétrica. As perdas na colheita mecanizada de feijão e a densidade de palhada apresentaram baixa variabilidade e distribuição normal. Assim, apenas o consumo horário e a produção de matéria seca de palhada apresentaram comportamento instável em relação ao controle estatístico de processo, enquanto os demais indicadores mostraram condições de manter a qualidade da operação de colheita tanto no preparo convencional de solo quanto no plantio direto.Among the production stages of the bean plant, harvesting is one of the most important, because it can decisively affect both quality and production costs. Thus, the objective was to assess quality in the mechanized harvesting of beans (Phaseolus vulgaris, grown under conventional tillage and no-tillage systems. The variables analysed were: the noise level emitted, calculated using a sound-pressure meter; the operational performance, by monitoring fuel consumption, wheel-slippage, and displacement velocity of the machine, all collected digitally (datalogger; and the harvesting operation with regard to the dry matter and

  1. Carbon Sequestration in Colorado's Lands: A Spatial and Policy Analysis

    Science.gov (United States)

    Brandt, N.; Brazeau, A.; Browning, K.; Meier, R.

    2017-12-01

    Managing landscapes to enhance terrestrial carbon sequestration has significant potential to mitigate climate change. While a previous carbon baseline assessment in Colorado has been published (Conant et al, 2007), our study pulls from the existing literature to conduct an updated baseline assessment of carbon stocks and a unique review of carbon policies in Colorado. Through a multi-level spatial analysis based in GIS and informed by a literature review, we established a carbon stock baseline and ran four land use and carbon stock projection scenarios using Monte Carlo simulations. We identified 11 key policy recommendations for improving Colorado's carbon stocks, and evaluated each using Bardach's policy matrix approach (Bardach, 2012). We utilized a series of case studies to support our policy recommendations. We found that Colorado's lands have a carbon stock of 3,334 MMT CO2eq, with Forests and Woodlands holding the largest stocks, at 1,490 and 774 MMT CO2eq respectively. Avoided conversion of all Grasslands, Forests, and Wetlands in Colorado projected over 40 years would increase carbon stocks by 32 MMT CO2eq, 1,053 MMT CO2eq, and 36 MMT CO2eq, respectively. Over the 40-year study period, Forests and Woodlands areas are projected to shrink while Shrublands and Developed areas are projected to grow. Those projections suggest sizable increases in area of future wildfires and development in Colorado. We found that numerous policy opportunities to sequester carbon exist at different jurisdictional levels and across land cover types. The largest opportunities were found in state-level policies and policies impacting Forests, Grasslands, and Wetlands. The passage of statewide emission reduction legislation has the highest potential to impact carbon sequestration, although political and administrative feasibility of this option are relatively low. This study contributes to the broader field of carbon sequestration literature by examining the nexus of carbon stocks

  2. Assessing Vulnerability under Uncertainty in the Colorado River Basin: The Colorado River Basin Water Supply and Demand Study

    Science.gov (United States)

    Jerla, C.; Adams, P.; Butler, A.; Nowak, K.; Prairie, J. R.

    2013-12-01

    Spanning parts of the seven states, of Arizona, California, Colorado, New Mexico, Nevada, Utah, and Wyoming, the Colorado River is one of the most critical sources of water in the western United States. Colorado River allocations exceed the long-term supply and since the 1950s, there have been a number of years when the annual water use in the Colorado River Basin exceeded the yield. The Basin is entering its second decade of drought conditions which brings challenges that will only be compounded if projections of climate change are realized. It was against this backdrop that the Colorado River Basin Water Supply and Demand Study was conducted. The Study's objectives are to define current and future imbalances in the Basin over the next 50 years and to develop and analyze adaptation and mitigation strategies to resolve those imbalances. Long-term planning in the Basin involves the integration of uncertainty with respect to a changing climate and other uncertainties such as future demand and how policies may be modified to adapt to changing reliability. The Study adopted a scenario planning approach to address this uncertainty in which thousands of scenarios were developed to encompass a wide range of plausible future water supply and demand conditions. Using Reclamation's long-term planning model, the Colorado River Simulation System, the reliability of the system to meet Basin resource needs under these future conditions was projected both with and without additional future adaptation strategies in place. System reliability metrics were developed in order to define system vulnerabilities, the conditions that lead to those vulnerabilities, and sign posts to indicate if the system is approaching a vulnerable state. Options and strategies that reduce these vulnerabilities and improve system reliability were explored through the development of portfolios. Four portfolios, each with different management strategies, were analyzed to assess their effectiveness at

  3. Bibliography of theses and dissertations on Colorado, 1968-1980

    International Nuclear Information System (INIS)

    Anon.

    1981-01-01

    This thesis bibliography for Colorado is the sixth (of 14) state bibliographies to be published in The Mountain Geologist by the R.M.A.G. Titles Committee. In an effort to update existing thesis publications and publicize more current work in the western United States, this R.M.A.G. thesis study covers only the last decade's theses and dissertations, specifically the period from 1968-1980. A total of 440 (Colorado) masters theses and dissertations have been compiled in this R.M.A.G. study for this period. Of these, 104 cover general or regional topics. In these cases, the titles are designated in the listings with asterisks (*) and have not been plotted on the accompanying location maps

  4. Triple-Layer Plastic Bags Protect Dry Common Beans (Phaseolus vulgaris) Against Damage by Acanthoscelides obtectus (Coleoptera: Chrysomelidae) During Storage.

    Science.gov (United States)

    Mutungi, C; Affognon, H D; Njoroge, A W; Manono, J; Baributsa, D; Murdock, L L

    2015-10-01

    Fumigated dry common beans (Phaseolus vulgaris L.) that were artificially infested with Acanthoscelides obtectus Say, and others that were not artificially infested, were stored in hermetic triple-layer PICS (Lela Agro, Kano, Nigeria) or woven polypropylene (PP) bags for 6 mo at ambient laboratory temperature conditions of 22.6 ± 1.9°C and 60.1 ± 4.3% relative humidity. In an additional trial, beans contained in PP bags were treated with Actellic Super dust before introducing A. obtectus. Moisture content, number of live adult A. obtectus, seed damage, weight loss, and seed germination were determined at monthly intervals. At 6 mo, beans stored in PICS bags retained higher moisture than those stored in PP bags, but in all treatments the moisture level remained below that recommended for safe storage of beans. In the PICS bags, proliferation of A. obtectus did not proceed and at 6 mo, beans stored in these bags did not have insect-inflicted seed damage or weight loss. In contrast, seed damage and weight loss in PP bags exceeded economic threshold after 1 mo in the absence of Actellic Super dust (Syngenta Crop protection AG, Basle, Switzerland), and after 2 mo in the presence of it. Germination of beans stored in PP bags decreased greatly whereas the beans stored in PICS bags did not show reduced germination. Chemical free storage of common beans in PICS bags protects them against damage by A. obtectus. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Low Soil Phosphorus Availability Increases Acid Phosphatases Activities and Affects P Partitioning in Nodules, Seeds and Rhizosphere of Phaseolus vulgaris

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    Jean-Jacques Drevon

    2012-06-01

    Full Text Available The effect of phosphorus (P deficiency on phosphatases activities in N2-fixing legumes has been widely studied in hydroponic culture. However, the response of acid phosphatase (APase and phytase in rhizosphere, nodules and seeds of Phaseolus vulgaris to low soil’s P-availability is not yet fully understood. In this study, six genotypes of N2-fixing P. vulgaris were grown under contrasting soil P-availabilities; i.e., low  (4.3 mg P kg−1 and sufficient (16.7 mg P kg−1 in the Haouz region of Morocco. At flowering and maturity stages, plants were harvested and analyzed for their phosphatases activities, growth and P content. Results show that, low P decreased nodulation, growth, P uptake and N accumulation in all the genotypes, but to a greater extent in the sensitive recombinant inbreed line 147. In addition, while seed P content was slightly reduced under low P soil; a higher P was noticed in the Flamingo and Contender large seeded-beans (6.15 to 7.11 mg g−1. In these latter genotypes, high APase and phytase activities in seeds and nodules were associated with a significant decline in rhizosphere’s available P. APase activity was mainly stimulated in nodules, whereas phytase activity was highly induced in seeds (77%. In conclusion, the variations of APase and phytase activities in nodules and seeds depend on genotype and can greatly influence the internal utilization of P, which might result in low P soil tolerance in N2-fixing legumes.

  6. Effect of the dietary level of cull pinto beans (Phaseolus vulgaris on ruminal fermentation, kinetics, and digestibility of hair lambs

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    Francisco Castillo Rangel

    Full Text Available ABSTRACT The objective was to evaluate the effect of three levels of cull pinto beans (CPB; Phaseolus vulgaris on ruminal fermentation, kinetics, and nutrient digestibility in hair lambs. Six cannulated lambs averaging 56.6±3.8 kg were used and were randomly assigned to one of three treatments. Treatments were: 0.0 kg kg−1 of CPB in the supplement (control; 0.25 kg kg−1 of CPB in the supplement (CB25; and 0.40 kg kg−1 of CPB in the supplement (CB40. Dry matter intake, ruminal pH, NH3, and volatile fatty acid (VFA concentration, methane production, Kp (passage rate, MRT (mean retention time, and digestibility of dry matter, crude protein, and neutral detergent fiber were evaluated. Data were analyzed in a Latin square design, repeated in line, by MIXED procedure of SAS. Estimates used for Kp and MRT were obtained by a non-linear regression model (PROC NLIN. Dry matter intake was reduced by supplementation of CPB. No differences were found in ruminal pH or ruminal NH3. During the trial, differences were found for ruminal VFA concentration (mM, which were greater for the CB25 group. The propionate:acetate ratio was greater for the CB40 treatment. Methane production (mM/m differed among treatments, but it was the greatest for the CB40 group. Passage rate (kg kg−1/h and MRT (h were similar among treatments and the digestibility (kg kg−1 of dry matter, crude protein, and neutral detergent fiber was not different among treatments. The inclusion of 0.25 kg kg−1 of CPB in the diet of hair lambs allows for appropriate nutrient digestion without affecting Kp and MRT and increases the molar proportion of the ability of VFA to maintain acetate:propionate ratio without increasing methane production.

  7. Metabolic origin of the {delta}{sup 13}C of respired CO{sub 2} in roots of Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Bathellier, C.; Tcherkez, G.; Cornic, G.; Ghashghaie, J. [Laboratoire d' Ecologie, Systematique et Evolution - ESE, CNRS-UMR 8079 - IFR 87, Batiment 362, Universite Paris-Sud, 91405-Orsay Cedex (France); Tcherkez, G. [Plateforme Metabolisme-Metabolome, IFR87 La Plante et son Environnement, Institut de Biotechnologie des Plantes, Batiment 630, Universite Paris-Sud, 91405-Orsay Cedex (France); Bligny, R.; Gout, E. [Laboratoire de Physiologie Cellulaire Vegetale CEA-Grenoble 17, rue des Martyrs, 38054 Grenoble Cedex 9 (France)

    2009-07-01

    - Root respiration is a major contributor to soil CO{sub 2} efflux, and thus an important component of ecosystem respiration. But its metabolic origin, in relation to the carbon isotope composition ({delta}{sup 13}C), remains poorly understood. - Here, {sup 13}C analysis was conducted on CO{sub 2} and metabolites under typical conditions or under continuous darkness in French bean (Phaseolus vulgaris) roots. {sup 13}C contents were measured either under natural abundance or following pulse-chase labeling with {sup 13}C-enriched glucose or pyruvate, using isotope ratio mass spectrometer (IRMS) and nuclear magnetic resonance (NMR) techniques. - In contrast to leaves, no relationship was found between the respiratory quotient and the {delta}{sup 13}C of respired CO{sub 2}, which stayed constant at a low value (c. -27.5 per thousand) under continuous darkness. With labeling experiments, it is shown that such a pattern is explained by the {sup 13}C-depleting effect of the pentose phosphate pathway; and the involvement of the Krebs cycle fueled by either the glycolytic input or the lipid/protein recycling. The anaplerotic phosphoenolpyruvate carboxylase (PEPc) activity sustained glutamic acid (Glu) synthesis, with no net effect on respired CO{sub 2}. - These results indicate that the root {delta}{sup 13}C signal does not depend on the availability of root respiratory substrates and it is thus plausible that, unless the {sup 13}C photosynthetic fractionation varies at the leaf level, the root {delta}{sup 13}C signal hardly changes under a range of natural environmental conditions. (authors)

  8. Ferti-irrigational impact of sugar mill effluent on agronomical characteristics of Phaseolus vulgaris (L.) in two seasons.

    Science.gov (United States)

    Kumar, Vinod; Chopra, A K

    2014-11-01

    Ferti-irrigation response of 5, 10, 25, 50, 75, and 100 % concentrations of the sugar mill effluent (SME) on French bean (Phaseolus vulgaris L., cv. Annapurna) in the rainy and summer seasons was investigated. The fertigant concentrations produced significant (P potassium (K(+)), calcium (Ca(2+)), magnesium (Mg(2+)), total Kjeldahl nitrogen (TKN), phosphate (PO4 (3-)), sulfate (SO4 (2-)), ferrous (Fe(2+)), cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), and zinc (Zn), in both seasons. The contents of Cr, Cu, Mn, and Zn except Cd were found to be below the maximum levels permitted for soils in India. The agronomic performance of P. vulgaris was gradually increased at lower concentrations, i.e., from 5 to 25 %, and decreased at higher concentrations, i.e., from 50 to 100 %, of the SME in both seasons when compared to controls. The accumulations of heavy metals were increased in the soil and P. vulgaris from 5 to 100 % concentrations of the SME in both seasons. The contents of Cu, Mn, and Zn except Cd and Cr were noted under the permissible limit of Food and Agriculture Organization (FAO)/World Health Organization (WHO) standards. Most contents of biochemical components like crude proteins, crude fiber, and total carbohydrates were found with 25 % concentration of the SME in both seasons. The contamination factor (Cf) of various metals was in the order of Cd > Cr > Zn > Mn > Cu for soil and Mn > Zn > Cu > Cr > Cd for P. vulgaris in both seasons after fertigation with SME. Therefore, the SME can be used to improve the soil fertility and yield of P. vulgaris after appropriate dilution.

  9. Specificity of induced defenses, growth, and reproduction in lima bean (Phaseolus lunatus) in response to multispecies herbivory.

    Science.gov (United States)

    Moreira, Xoaquín; Abdala-Roberts, Luis; Hernández-Cumplido, Johnattan; Cuny, Maximilien A C; Glauser, Gaetan; Benrey, Betty

    2015-08-01

    • Following herbivore attack, plants can either reduce damage by inducing defenses or mitigate herbivory effects through compensatory growth and reproduction. It is increasingly recognized that such induced defenses in plants are herbivore-specific, but less is known about the specificity of compensatory responses. Damage by multiple herbivores may also lead to synergistic effects on induction and plant fitness that differ from those caused by a single herbivore species. Although largely unstudied, the order of arrival and damage by different herbivore species might also play an important role in the impacts of herbivory on plants.• We investigated the specificity of defense induction (phenolics) and effects on growth (number of stems and leaves) and reproduction (number of seeds, seed mass, and germination rate) from feeding by two generalist leaf-chewing herbivores (Spodoptera eridania and Diabrotica balteata) on Phaseolus lunatus plants and evaluated whether simultaneous attack by both herbivores and their order of arrival influenced such dynamics.• Herbivory increased levels of leaf phenolics, but such effects were not herbivore-specific. In contrast, herbivory enhanced seed germination in an herbivore-specific manner. For all variables measured, the combined effects of both herbivore species did not differ from their individual effects. Finally, the order of herbivore arrival did not influence defense induction, plant growth, or seed number but did influence seed mass and germination.• Overall, this study highlights novel aspects of the specificity of plant responses induced by damage from multiple species of herbivores and uniquely associates such effects with plant lifetime fitness. © 2015 Botanical Society of America, Inc.

  10. Annotation, Phylogeny and Expression Analysis of the Nuclear Factor Y Gene Families in Common Bean (Phaseolus vulgaris

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    Carolina eRípodas

    2015-01-01

    Full Text Available In the past decade, plant nuclear factor Y (NF-Y genes have gained major interest due to their roles in many biological processes in plant development or adaptation to environmental conditions, particularly in the root nodule symbiosis established between legume plants and nitrogen fixing bacteria. NF-Ys are heterotrimeric transcriptional complexes composed of three subunits, NF-YA, NF-YB and NF-YC, which bind with high affinity and specificity to the CCAAT box, a cis element present in many eukaryotic promoters. In plants, NF-Y subunits consist of gene families with about ten members each. In this study, we have identified and characterized the NF-Y gene families of common bean (Phaseolus vulgaris, a grain legume of worldwide economical importance and the main source of dietary protein of developing countries. Expression analysis showed that some members of each family are up-regulated at early or late stages of the nitrogen fixing symbiotic interaction with its partner Rhizobium etli. We also showed that some genes are differentially accumulated in response to inoculation with high or less efficient R. etli strains, constituting excellent candidates to participate in the strain-specific response during symbiosis. Genes of the NF-YA family exhibit a highly structured intron-exon organization. Moreover, this family is characterized by the presence of upstream ORFs when introns in the 5' UTR are retained and miRNA target sites in their 3' UTR, suggesting that these genes might be subjected to a complex post-transcriptional regulation. Multiple protein alignments indicated the presence of highly conserved domains in each of the NF-Y families, presumably involved in subunit interactions and DNA binding. The analysis presented here constitutes a starting point to understand the regulation and biological function of individual members of the NF-Y families in different developmental processes in this grain legume.

  11. Localization of the Bacillus subtilis beta-propeller phytase transcripts in nodulated roots of Phaseolus vulgaris supplied with phytate.

    Science.gov (United States)

    Maougal, Rim Tinhinen; Bargaz, Adnane; Sahel, Charaf; Amenc, Laurie; Djekoun, Abdelhamid; Plassard, Claude; Drevon, Jean-Jacques

    2014-04-01

    Soil organic phosphorus (Po) such as phytate, which comprises up to 80 % of total Po, must be hydrolyzed by specific enzymes called phytases to be used by plants. In contrast to plants, bacteria, such as Bacillus subtilis, have the ability to use phytate as the sole source of P due to the excretion of a beta-propeller phytase (BPP). In order to assess whether the B. subtilis BPP could make P available from phytate for the benefit of a nodulated legume, the P-sensitive recombinant inbred line RIL147 of Phaseolus vulgaris was grown under hydroaeroponic conditions with either 12.5 μM phytate (C₆H₁₈O₂₄P₆) or 75 μmol Pi (K₂HPO₄), and inoculated with Rhizobium tropici CIAT899 alone, or co-inoculated with both B. subtilis DSM 10 and CIAT899. The in situ RT-PCR of BPP genes displayed the most intense fluorescent BPP signal on root tips. Some BPP signal was found inside the root cortex and the endorhizosphere of the root tip, suggesting endophytic bacteria expressing BPP. However, the co-inoculation with B. subtilis was associated with a decrease in plant P content, nodulation and the subsequent plant growth. Such a competitive effect of B. subtilis on P acquisition from phytate in symbiotic nitrogen fixation might be circumvented if the rate of inoculation were reasoned in order to avoid the inhibition of nodulation by excess B. subtilis proliferation. It is concluded that B. subtilis BPP gene is expressed in P. vulgaris rhizosphere.

  12. beta. -1,4-glucan occurring in homogenate of Phaseolus aureus seedlings. Possible nascent stage of cellulose biosynthesis in vivo

    Energy Technology Data Exchange (ETDEWEB)

    Satoh, S; Matsuda, K; Tamari, K

    1976-12-01

    A small amount of cytoplasmic ..beta..-1,4-glucan, which might be involved in the synthesis of cellulose in the cell wall, was found in the homogenate prepared from the hypocotyls of seedlings of Phaseolus aureus. Upon hydrolysis by cellulase of the 20,000xg pellet from the cytoplasmic fraction of segments incubated in a (/sup 14/C)-glucose solution, (/sup 14/C)-cellobiose was produced, with specific radioactivities 3 to 10 times greater than those of the cellobiose from cellulose in the cell wall at various incubation periods. The incoporation of radioactivity from (/sup 14/C)-glucose into this cytoplasmic ..beta..-1,4-glucan was therefore faster than that into cellulose constituting the cell wall. Hence, it seemed that the former ..beta..-1,4-glucan could be turned over. To examine whether the cytoplasmic ..beta..-1,4-glucan is carried by some subcellular components, cytoplasmic ..beta..-1,4-glucan in the cell was fractionated by differential centrifugation, two enzyme activities being measured as the markers of subcellular components. The distribution of ..beta..-1,4-glucan was similar to that of UDPG-glucosyl-transferase activity but not to that of IDP-ase activity. The result suggests that the cytoplasmic ..beta..-1,4-glucan has some relation to plasma membranes. Coumarin, known as a specific inhibitor for the biosynthesis of cellulose in plant cells, was shown to inhibit the incorporation of radio-carbon from (/sup 14/C)-glucose into cytoplasmic ..beta..-1,4-glucan to the same extent as that into cellulose in the cell wall of the hypocotyls.

  13. Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Juliana Morini Küpper Cardoso Perseguini

    Full Text Available The common bean (Phaseolus vulgaris L. is the world's most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum and angular leaf spot (ALS; Pseudocercospora griseola are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genome-wide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will

  14. Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Mingli Chen

    Full Text Available Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.. The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY. The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.

  15. Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Chen, Mingli; Wu, Jing; Wang, Lanfen; Mantri, Nitin; Zhang, Xiaoyan; Zhu, Zhendong; Wang, Shumin

    2017-01-01

    Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.

  16. Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas.

    Science.gov (United States)

    Choudhary, Neeraj; Bawa, Vanya; Paliwal, Rajneesh; Singh, Bikram; Bhat, Mohd Ashraf; Mir, Javid Iqbal; Gupta, Moni; Sofi, Parvaze A; Thudi, Mahendar; Varshney, Rajeev K; Mir, Reyazul Rouf

    2018-01-01

    Common bean (Phaseolus vulgaris L.) is one of the most important grain legume crops in the world. The beans grown in north-western Himalayas possess huge diversity for seed color, shape and size but are mostly susceptible to Anthracnose disease caused by seed born fungus Colletotrichum lindemuthianum. Dozens of QTLs/genes have been already identified for this disease in common bean world-wide. However, this is the first report of gene/QTL discovery for Anthracnose using bean germplasm from north-western Himalayas of state Jammu & Kashmir, India. A core set of 96 bean lines comprising 54 indigenous local landraces from 11 hot-spots and 42 exotic lines from 10 different countries were phenotyped at two locations (SKUAST-Jammu and Bhaderwah, Jammu) for Anthracnose resistance. The core set was also genotyped with genome-wide (91) random and trait linked SSR markers. The study of marker-trait associations (MTAs) led to the identification of 10 QTLs/genes for Anthracnose resistance. Among the 10 QTLs/genes identified, two MTAs are stable (BM45 & BM211), two MTAs (PVctt1 & BM211) are major explaining more than 20% phenotypic variation for Anthracnose and one MTA (BM211) is both stable and major. Six (06) genomic regions are reported for the first time, while as four (04) genomic regions validated the already known QTL/gene regions/clusters for Anthracnose. The major, stable and validated markers reported during the present study associated with Anthracnose resistance will prove useful in common bean molecular breeding programs aimed at enhancing Anthracnose resistance of local bean landraces grown in north-western Himalayas of state Jammu and Kashmir.

  17. Genome-Wide Association Studies of Anthracnose and Angular Leaf Spot Resistance in Common Bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Perseguini, Juliana Morini Küpper Cardoso; Oblessuc, Paula Rodrigues; Rosa, João Ricardo Bachega Feijó; Gomes, Kleber Alves; Chiorato, Alisson Fernando; Carbonell, Sérgio Augusto Morais; Garcia, Antonio Augusto Franco; Vianello, Rosana Pereira; Benchimol-Reis, Luciana Lasry

    2016-01-01

    The common bean (Phaseolus vulgaris L.) is the world's most important legume for human consumption. Anthracnose (ANT; Colletotrichum lindemuthianum) and angular leaf spot (ALS; Pseudocercospora griseola) are complex diseases that cause major yield losses in common bean. Depending on the cultivar and environmental conditions, anthracnose and angular leaf spot infections can reduce crop yield drastically. This study aimed to estimate linkage disequilibrium levels and identify quantitative resistance loci (QRL) controlling resistance to both ANT and ALS diseases of 180 accessions of common bean using genome-wide association analysis. A randomized complete block design with four replicates was performed for the ANT and ALS experiments, with four plants per genotype in each replicate. Association mapping analyses were performed for ANT and ALS using a mixed linear model approach implemented in TASSEL. A total of 17 and 11 significant statistically associations involving SSRs were detected for ANT and ALS resistance loci, respectively. Using SNPs, 21 and 17 significant statistically associations were obtained for ANT and angular ALS, respectively, providing more associations with this marker. The SSR-IAC167 and PvM95 markers, both located on chromosome Pv03, and the SNP scaffold00021_89379, were associated with both diseases. The other markers were distributed across the entire common bean genome, with chromosomes Pv03 and Pv08 showing the greatest number of loci associated with ANT resistance. The chromosome Pv04 was the most saturated one, with six markers associated with ALS resistance. The telomeric region of this chromosome showed four markers located between approximately 2.5 Mb and 4.4 Mb. Our results demonstrate the great potential of genome-wide association studies to identify QRLs related to ANT and ALS in common bean. The results indicate a quantitative and complex inheritance pattern for both diseases in common bean. Our findings will contribute to more

  18. REACTION OF INTRODUCED BEAN (PHASEOLUS ACCESSIONS TO THE INFESTATION BY THIELAVIOPSIS BASICOLA (BERKELEY & BROOME UNDER NATURAL EPIPHYTOTIC CONDITIONS

    Directory of Open Access Journals (Sweden)

    O. Georgieva

    2015-01-01

    Full Text Available A periodic phytopathology field monitoring was conducted on 35 introduced common bean (Phaseolus accessions at Maritsa Vegetable Crops Research Institute in 2014. The epiphytotic disease black root rot on the bean crops (over 75 % reduction of the stand was recorded for the first time for the area of Bulgaria. The causal agent isolated from the plant tissue was identified as the fungus Thielaviopsis basicola (Berkeley Ferraris. A strong relationship between disease severity variation and environmental and soil conditions was established. Black root rot was most severe when cool and wet weather occurred from seedling time to about three weeks after planting, combined with increased soil compaction. Field resistance was recorded in Bulgarian var. “Plovdivski zult”, var. “Starozagorski tzer” and line № 564 (3,66%, 5.33% and 6,50 % dumping-off of bean seedlings, respectively. Bean accession introduced from dry climate areas were highly susceptible to black root rot pathogen (over 76.0 % dumping-off of bean seedlings. Indirect relationship was found between bean tolerance to Th. basicola and presence of the anthocyanin in the hypocotyl and seed coat color. Install the average negative correlation between seed color signs (and hypocotyl and the resistance of plants to Th. basicola. Samples with resistance to black root rot belong to the group with beige, red, brown or black color of seeds. The presence of phenolic compounds (anthocyanins in the seed coat and hypocotyls beans can serve as an indirect indication of the selection of resistant to black rot breeding materials.

  19. Antioxidant and Hypolipidaemic Effect of Raw Green Snap (Phaseolus vulgaris) on Aged Male Rats Exposed to gamma Irradiation

    International Nuclear Information System (INIS)

    Abed EI-Gawad, E.I.; Aiad, S.K.

    2008-01-01

    This Study was established to assess the effect of supplemental dietary raw green snap (Phaseolus vulgaris) for three months to overcome gamma-irradiation induced alterations on some oxidant/ antioxidant parameters (Fe 3+ , Cu 2+ , total iron, ferritin, transferrin and ceruloplasmin) and the hyper lipidemic state (triglycerides, cholesterol, high- and low density lipoprotein (HDL-c and LDL-c) in aged male rats. Raw green snap is an indigenous plant used in Unani and Ayurvedic medicine in India. The study also aimed to estimate the effect of dietary raw green snap on general health through the follow up of body weight and mortality during the course of supplementation. Thirty-two aged male rats (24 months, 370-375 g) were divided equally into four groups. 1- Control group, the animals fed on a balanced diet for 3 months. 2- Supplemented group, the animals balanced diet was supplemented with raw green snap (70 g / kg body wt/ day) for 3 months. 3- Irradiated group, the animals fed on a balanced diet for 3 months were then exposed to whole body y-radiation at a dose level of 4 Gy. 4-Supplemented-irradiated group, the animals' balanced diet was supplemented with raw green snap (70 g/ kg/ day) for three months and then exposed to whole body gamma irradiation at a dose level of 4 Gy. The blood samples were taken from orbital venous plexuses after 48 h of stopping green snap supplementation (supplemented group) or after irradiation (irradiated and supplemented gamma-irradiated groups). The results obtained showed reduction in the body wt in green snap supplemented group which increased gradually concomitant with occurrence of animal mortality on week 7 reaching II) maximal values (-32.79 and 33.33 %) respectively, on week 12 of supplementation. Also, the supplemented group showed non significant changes in tested parameters although the level of total iron and triglycerides recorded noticeable changes as compared with controls

  20. Rapid evolutionary change of common bean (Phaseolus vulgaris L plastome, and the genomic diversification of legume chloroplasts

    Directory of Open Access Journals (Sweden)

    Dávila Guillermo

    2007-07-01

    Full Text Available Abstract Background Fabaceae (legumes is one of the largest families of flowering plants, and some members are important crops. In contrast to what we know about their great diversity or economic importance, our knowledge at the genomic level of chloroplast genomes (cpDNAs or plastomes for these crops is limited. Results We sequenced the complete genome of the common bean (Phaseolus vulgaris cv. Negro Jamapa chloroplast. The plastome of P. vulgaris is a 150,285 bp circular molecule. It has gene content similar to that of other legume plastomes, but contains two pseudogenes, rpl33 and rps16. A distinct inversion occurred at the junction points of trnH-GUG/rpl14 and rps19/rps8, as in adzuki bean 1. These two pseudogenes and the inversion were confirmed in 10 varieties representing the two domestication centers of the bean. Genomic comparative analysis indicated that inversions generally occur in legume plastomes and the magnitude and localization of insertions/deletions (indels also vary. The analysis of repeat sequences demonstrated that patterns and sequences of tandem repeats had an important impact on sequence diversification between legume plastomes and tandem repeats did not belong to dispersed repeats. Interestingly, P. vulgaris plastome had higher evolutionary rates of change on both genomic and gene levels than G. max, which could be the consequence of pressure from both mutation and natural selection. Conclusion Legume chloroplast genomes are widely diversified in gene content, gene order, indel structure, abundance and localization of repetitive sequences, intracellular sequence exchange and evolutionary rates. The P. vulgaris plastome is a rapidly evolving genome.

  1. Damaged-self recognition in common bean (Phaseolus vulgaris shows taxonomic specificity and triggers signalling via reactive oxygen species (ROS

    Directory of Open Access Journals (Sweden)

    Dalia eDuran

    2014-10-01

    Full Text Available Plants require reliable mechanisms to detect injury. Danger signals or 'damage-associated molecular patterns' (DAMPs are released from stressed host cells and allow injury detection independently of enemy-derived molecules. We studied the response of common bean (Phaseolus vulgaris to the application of leaf homogenate as a source of DAMPs and measured the production of reactive oxygen species (ROS as an early response and the secretion of extrafloral nectar (EFN as a jasmonic acid (JA–dependent late response. We observed a strong taxonomic signal in the response to different leaf homogenates. ROS formation and EFN secretion were highly correlated and responded most strongly to leaf homogenates produced using the same cultivar or closely related accessions, less to a distantly related cultivar of common bean or each of the two congeneric species, P. lunatus and P. coccineus, and not at all to homogenates prepared from species in different genera, not even when using other Fabaceae. Interestingly, leaf homogenates also reduced the infection by the bacterial pathogen, Pseudomonas syringae, when they were applied directly before challenging, although the same homogenates exhibited no direct in vitro inhibitory effect in the bacterium. We conclude that ROS signaling is associated to the induction of EFN secretion and that the specific blend of DAMPs that are released from damaged cells allows the plant to distinguish the 'damaged self' from the damaged 'non-self'. The very early responses of plants to DAMPs can trigger resistance to both, herbivores and pathogens, which should be adaptive because injury facilitates infection, independently of its causal reason.

  2. Response- Surface Analysis for Evaluation of Competition in Different Densities of Sesame (Sesamum indicum and Bean (Phaseolus vulgaris Intercropping

    Directory of Open Access Journals (Sweden)

    A. Koocheki

    2014-12-01

    Full Text Available Response surface models predict crop yield based on crop density and this is an important tool for evaluation competition at different density and hence selection of optimum density based on yield. In order to study intra and inter specific competition in intercropping bean (Phaseolus vulgaris and sesame (Sesamum indicum, an experiment was conducted at the Agricultural Research Station, Ferdowsi University of Mashhad during the growing season of 2010. For this purpose a complete randomized block design with 3 replications and 16 treatments based on different densities of sesame and bean intercropping was used. The model predicted the maximum yield of an isolated plant of bean and sesame approximately 33 and 17g per plant respectively. The area associated with the maximum yield per plant in bean and sesame were 0.6 and 0.1 m2, respectively. Bean was the dominant competitor with respect to both grain and biomass, and competition coefficient was 0.35 and 0.3 for bean grain yield and bean biomass respectively. Intra-specific competition was more important than inter-specific competition for bean. Competition coefficient was 2.6 and 2.9 for sesame grain yield and biomass respectively. Intra-specific competition was much less important than Interspecific competition in sesame. The highest grain yield in bean (300 g m-2 was obtained of sole crop with density of 20 plants, and the highest sesame grain yield (195 g m-2 was obtained of sole crop with density of 40 plants, the highest land equivalent ratio (1.14 was obtained in intercropping of 20 plants of bean and 10 plants of sesame.

  3. Conformational study of red kidney bean (Phaseolus vulgaris L.) protein isolate (KPI) by tryptophan fluorescence and differential scanning calorimetry.

    Science.gov (United States)

    Yin, Shou-Wei; Tang, Chuan-He; Yang, Xiao-Quan; Wen, Qi-Biao

    2011-01-12

    Fluorescence and differential scanning calorimetry (DSC) were used to study changes in the conformation of red kidney bean (Phaseolus vulgaris L.) protein isolate (KPI) under various environmental conditions. The possible relationship between fluorescence data and DSC characteristics was also discussed. Tryptophan fluorescence and fluorescence quenching analyses indicated that the tryptophan residues in KPI, exhibiting multiple fluorophores with different accessibilities to acrylamide, are largely buried in the hydrophobic core of the protein matrix, with positively charged side chains close to at least some of the tryptophan residues. GdnHCl was more effective than urea and SDS in denaturing KPI. SDS and urea caused variable red shifts, 2-5 nm, in the emission λ(max), suggesting the conformational compactness of KPI. The result was further supported by DSC characteristics that a discernible endothermic peak was still detected up to 8 M urea or 30 mM SDS, also evidenced by the absence of any shift in emission maximum (λ(max)) at different pH conditions. Marked decreases in T(d) and enthalpy (ΔH) were observed at extreme alkaline and/or acidic pH, whereas the presence of NaCl resulted in higher T(d) and ΔH, along with greater cooperativity of the transition. Decreases in T(d) and ΔH were observed in the presence of protein perturbants, for example, SDS and urea, indicating partial denaturation and decrease in thermal stability. Dithiothreitol and N-ethylmaleimide have a slight effect on the thermal properties of KPI. Interestingly, a close linear relationship between the T(d) (or ΔH) and the λ(max) was observed for KPI in the presence of 0-6 M urea.

  4. Daya Simpan Susu Kacang Hijau (Phaseolus radiatus L. dengan Persentase Penambahan Sari Jahe Merah (Zingiber officinale var. Rubrum

    Directory of Open Access Journals (Sweden)

    Meilla Dwi Andrestian

    2015-06-01

    Mung bean milk is one of the diversification of processed mung beans. The process of making mung bean milk using Ultra High Temperature (UHT is abundantly sold in the market. As another alternative to have a high shelf life, it needs the addition of natural preservatives such as red ginger. In general, this research aim was to determine the effect of addition of the percentage of red ginger extract (Zingiber officinale var. Rubrum on storability of green beans milk (Phaseolus radiatus L.. This research consisted of two phases, preliminary research and main research. The former stage was conducted to determine the best acceptability of nine treatment variations of red ginger extract addition. After preliminary research was made and it obtained the best results preferred by panelist, it was then followed by main research to determine the storability of green beans milk. From preliminary research, theres were three treatment preferred by panelists, namely P3 (0.75%, P5 (1.25% and P6 (1.5%. After that, those  treatments were tested for their storability in main research. From main research it showed that Q0 (control treatment can last for 0 day, Q1 treatment with the addition of red ginger extract 0,75% and Q2 (1.25% having storability for 1 day and Q3 (1.5% having the best treatment that had the longest storability (for 2 days. The more addition of red ginger extract, the longer storability of green beans milk. The best favored and longest storability of green beans milk was one added with red ginger extract of 1.5%. Keywords: Green Bean Milk, Storability, Red ginger

  5. Uranium guidebook for the Paradox Basin, Utah and Colorado

    International Nuclear Information System (INIS)

    Anon.

    1978-01-01

    Various data concerning uranium production and reserves of the Paradox Basin are compiled herein. Current production from 2 Utah mills and 1 Colorado mill is estimated at 5.4 million pounds of U 3 O 8 . An overview of uranium mining, geology, tectonics, and types of ore deposits is presented. Detailed description of the 11 mining districts and 21 mining areas are included. Detailed maps and exploration guides are included as assistance to evaluating and seeking new uranium deposits

  6. Multicriteria GIS modeling of wind and solar farms in Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Janke, Jason R. [Metropolitan State College of Denver, Department of Earth and Atmospheric Sciences, CB 22 P.O. Box 173362-22, Denver, CO 80217-3362 (United States)

    2010-10-15

    The majority of electricity and heat in Colorado comes from coal and natural gas; however, renewable energy sources will play an integral role in the state's energy future. Colorado is the 11th windiest state and has more than 250 sunny days per year. The objectives of this research are to: 1) determine which landcover classes are affiliated with high wind and solar potential; and 2) identify areas that are suitable for wind and solar farms using multicriteria GIS modelling techniques. Renewable potential (NREL wind speed measurements at 50 m above the ground and NREL annual insolation data), landcover, population density, federal lands, and distance to roads, transmission lines, and cities were reclassified according to their suitability. Each was assigned weights based on their relative importance to one another. Superb wind classes are located in high alpine areas. Unfortunately, these areas are not suitable for large-scale wind farm development due to their inaccessibility and location within a sensitive ecosystem. Federal lands have low wind potential. According to the GIS model, ideal areas for wind farm development are located in northeastern Colorado. About 41 850 km{sup 2} of the state has model scores that are in the 90-100% range. Although annual solar radiation varies slightly, inter-mountain areas receive the most insolation. As far as federal lands, Indian reservations have the greatest solar input. The GIS model indicates that ideal areas for solar development are located in northwestern Colorado and east of Denver. Only 191 km{sup 2} of the state had model scores that were in the 90-100% range. These results suggest that the variables used in this analysis have more of an effect at eliminating non-suitable areas for large-scale solar farms; a greater area exists for suitable wind farms. However, given the statewide high insolation values with minimal variance, solar projects may be better suited for small-scale residential or commercial

  7. Estimated Colorado Golf Course Irrigation Water Use, 2005

    Science.gov (United States)

    Ivahnenko, Tamara

    2009-01-01

    Golf course irrigation water-use data were collected as part of the U.S. Geological Survey National Water Use Program's 2005 compilation to provide baseline information, as no golf course irrigation water-use data (separate from crop irrigation) have been reported in previous compilations. A Web-based survey, designed by the U.S. Geological Survey, in cooperation with the Rocky Mountain Golf Course Superintendents Association (RMGCSA), was electronically distributed by the association to the 237 members in Colorado. Forty-three percent of the members returned the survey, and additional source water information was collected by telephone for all but 20 of the 245 association member and non-member Colorado golf courses. For golf courses where no data were collected at all, an average 'per hole' coefficient, based on returned surveys from that same county, were applied. In counties where no data were collected at all, a State average 'per hole' value of 13.2 acre-feet was used as the coefficient. In 2005, Colorado had 243 turf golf courses (there are 2 sand courses in the State) that had an estimated 2.27 acre-feet per irrigated course acre, and 65 percent of the source water for these courses was surface water. Ground water, potable water (public supply), and reclaimed wastewater, either partially or wholly, were source waters for the remaining courses. Fifty-three of the 64 counties in Colorado have at least one golf course, with the greatest number of courses in Jefferson (23 courses), Arapahoe (22 courses), and El Paso Counties (20 courses). In 2005, an estimated 5,647.8 acre-feet in Jefferson County, 5,402 acre-feet in Arapahoe County, and 4,473.3 acre-feet in El Paso County were used to irrigate the turf grass.

  8. The historical distribution of Gunnison Sage-Grouse in Colorado

    Science.gov (United States)

    Braun, Clait E.; Oyler-McCance, Sara J.; Nehring, Jennifer A.; Commons, Michelle L.; Young, Jessica R.; Potter, Kim M.

    2014-01-01

    The historical distribution of Gunnison Sage-Grouse (Centrocercus minimus) in Colorado is described based on published literature, observations, museum specimens, and the known distribution of sagebrush (Artemisia spp.). Historically, Gunnison Sage-Grouse were widely but patchily distributed in up to 22 counties in south-central and southwestern Colorado. The historical distribution of this species was south of the Colorado-Eagle river drainages primarily west of the Continental Divide. Potential contact areas with Greater Sage-Grouse (C. urophasianus) were along the Colorado-Eagle river system in Mesa, Garfield, and Eagle counties, west of the Continental Divide. Gunnison Sage-Grouse historically occupied habitats that were naturally highly fragmented by forested mountains and plateaus/mesas, intermountain basins without robust species of sagebrush, and river systems. This species adapted to use areas with more deciduous shrubs (i.e., Quercus spp., Amelanchier spp., Prunus spp.) in conjunction with sagebrush. Most areas historically occupied were small, linear, and patchily distributed within the overall landscape matrix. The exception was the large intermountain basin in Gunnison, Hinsdale, and Saguache counties. The documented distribution east of the Continental Divide within the large expanse of the San Luis Valley (Alamosa, Conejos, Costilla, and Rio Grande counties) was minimal and mostly on the eastern, northern, and southern fringes. Many formerly occupied habitat patches were vacant by the mid 1940s with extirpations continuing to the late 1990s. Counties from which populations were recently extirpated include Archuleta and Pitkin (1960s), and Eagle, Garfield, Montezuma, and Ouray (1990s).

  9. Foraging Behavior of Odontomachus bauri on Barro Colorado Island, Panama

    Directory of Open Access Journals (Sweden)

    Birgit Ehmer

    1995-01-01

    Full Text Available Foraging behavior and partitioning of foraging areas of Odonomachus bauri were investigated on Barro Colorado Island in Panama. The activity of the ants did not show any daily pattern; foragers were active day and night. The type of prey captured by O. bauri supports the idea that in higher Odontomachus and Anochetus species, the high speed of mandible closure serves more for generating power than capturing elusive prey. Polydomous nests may enable O. bauri colonies to enlarge their foraging areas.

  10. Dynamics Days US 2013 Conference Held in Denver, Colorado on 3-6 January 2013. Abstracts

    Science.gov (United States)

    2013-12-17

    down before population collapse using replicate laboratory populations of the budding yeast Saccharomyces cerevisiae. We mapped the bifurcation diagram...agreement with the theory. Furthermore, we connected yeast populations spatially to evaluate warning signals based on spatio-temporal fluctuations...Colorado School of Mines $125 ARO 54 Franson, Andrew U Colorado School of Mines $125 ARO 55 Slater, Michael U Colorado School of Mines $125 ARO 56 Brewer

  11. Relationship between seed coat colors and patterns with phenolic content and antioxidant activity in a collection of 120 heirloom accessions of beans (Phaseolus vulgaris) from the National Plant Germplasm System.

    Science.gov (United States)

    Beans (Phaseolus vulgaris L.) are one of the most economically and nutritionally important crops world-wide. They are the most important legume for direct human consumption with more than 23 million metric tons produced in 2013; more than twice that of the next most important legume, chickpea (Cicer...

  12. Projection from the prefrontal cortex to histaminergic cell groups in the posterior hypothalamic region of the rat. Anterograde tracing with Phaseolus vulgaris leucoagglutinin combined with immunocytochemistry of histidine decarboxylase

    NARCIS (Netherlands)

    Wouterlood, F.G.; Steinbusch, H.W.M.; Luiten, P.G.M.; Bol, J.G.J.M.

    1987-01-01

    We investigated the projection from the infralimbic division of the prefrontal cortex (area 25) to histaminergic neurons in the posterior hypothalamic area. Phaseolus vulgaris-leucoagglutinin (PHA-L) was injected in the prefrontal cortex of rats. Frozen brain sections were subjected to combined

  13. Fabenol® Max, a standardised aqueous extract from Phaseolus vulgaris L., and ‘reduces the absorption of carbohydrates’: evaluation of a health claim pursuant toArticle 13(5) of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Sjödin, Anders Mikael

    2016-01-01

    on the scientific substantiation of a health claim related to Fabenol® Max and ‘reduces the absorption of carbohydrates’. The Panel considers that Fabenol® Max, which is an aqueous extract from Phaseolus vulgaris L. standardised by its content of α-amylase inhibitor, is sufficiently characterised. According...

  14. Rhizobia from Lanzarote, the Canary Islands, that nodulate Phaseolus vulgaris have characteristics in common with LMW RNA group II Sinorhizobium meliloti of Medicago, Melilotus and Trigonella from soils of mainland Spain

    Science.gov (United States)

    Several isolates from nodules of Phaseolus vulgaris grown in soil of Lanzarote, an island of the Canaries, had electrophoretic LMW RNA patterns identical with a less common pattern within S. meliloti (assigned as group II) obtained from nodules of alfalfa and alfalfa-related legumes grown in northe...

  15. Fitorremediación de suelos contaminados con metales pesados

    OpenAIRE

    Maqueda Gálvez, Alma Patricia

    2003-01-01

    En el presente trabajo se cultivaron plántulas de Beta vulgaris (acelga) , Phaseolus vulgaris L. (frijol) y Petroselinum crispum (perejil) a nivel de laboratorio. Se aplicaron cuatro tratamientos: T1 (50 mg Pb/kg de vermiculita y 2 mg Cd/kg de vermiculita), T2 (75 mg Pb/ kg de vermiculita y 2 mg Cd/kg de vermiculita), T3 (150 mg Pb/kg de vermiculita y 2 mg Cd/kg de vermiculita) y suelo contaminado, SC (208.19 mg Pb/kg y 10.04 mg Cd/kg. Las plántulas de acelga y de frijol se ...

  16. Deep mantle forces and the uplift of the Colorado Plateau

    Energy Technology Data Exchange (ETDEWEB)

    Moucha, R; Forte, A M; Rowley, D B; Mitrovica, J X; Simmons, N A; Grand, S P

    2009-06-23

    Since the advent of plate tectonics, it has been speculated that the northern extension of the East Pacific Rise, specifically its mantle source, has been over-ridden by the North American Plate in the last 30 Myrs. Consequently, it has also been postulated that the opening of the Gulf of California, the extension in the Basin and Range province, and the uplift of the Colorado Plateau are the resulting continental expressions of the over-ridden mantle source of the East Pacific Rise. However, only qualitative models based solely on surface observations and heuristic, simplified conceptions of mantle convection have been used in support or against this hypothesis. We introduce a quantitative model of mantle convection that reconstructs the detailed motion of a warm mantle upwelling over the last 30 Myrs and its relative advance towards the interior of the southwestern USA. The onset and evolution of the crustal uplift in the central Basin and Range province and the Colorado Plateau is determined by tracking the topographic swell due to this mantle upwelling through time. We show that (1) the extension and magmatism in the central Basin and Range province between 25 and 10 Ma coincides with the reconstructed past position of this focused upwelling, and (2) the southwestern portion of the Colorado Plateau experienced significant uplift between 10 Ma and 5 Ma that progressed towards the northeastern portion of the plateau. These uplift estimates are consistent with a young, ca. 6 Ma, Grand Canyon model and the recent commencement of mafic magmatism.

  17. Uranium exploration of the Colorado Plateau: interim staff report

    International Nuclear Information System (INIS)

    1980-10-01

    This report is an issue of the original draft copy of the Interim Staff Report on Uranium Exploration on the Colorado Plateau, dated June 1951. The original draft copy was only recently located and is being published at this time because of the interest in the contained historical content. The table of contents of this report lists: history of uranium mining; geology; proposed program for the geologic investigations section; general activities of industry and government; and future exploration of sedimentary uranium deposits and anticipated results. Under the proposed program section are: future of the copper-uranium deposits as a source of uranium; uraniferous asphaltite deposits; and commission exploration and future possibilities. The section on general activities of industry and government includes: exploratory and development drilling; field investigations and mapping; early geologic investigations and investigations by the US geological survey; and geophysical exploration. Tables are also presented on: uranium production by districts; US Geological survey drilling statistics; Colorado Exploration Branch drilling statistics; summary of drilling projects; and comparative yearly core-drill statistics on the Colorado Plateau

  18. Geothermal resource assessment of western San Luis Valley, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Zacharakis, Ted G.; Pearl, Richard Howard; Ringrose, Charles D.

    1983-01-01

    The Colorado Geological Survey initiated and carried out a fully integrated assessment program of the geothermal resource potential of the western San Luis Valley during 1979 and 1980. The San Luis Valley is a large intermontane basin located in southcentral Colorado. While thermal springs and wells are found throughout the Valley, the only thermal waters found along the western part of the Valley are found at Shaw Warm Springs which is a relatively unused spring located approximately 6 miles (9.66 km) north of Del Norte, Colorado. The waters at Shaws Warm Spring have a temperature of 86 F (30 C), a discharge of 40 gallons per minute and contain approximately 408 mg/l of total dissolved solids. The assessment program carried out din the western San Luis Valley consisted of: soil mercury geochemical surveys; geothermal gradient drilling; and dipole-dipole electrical resistivity traverses, Schlumberger soundings, Audio-magnetotelluric surveys, telluric surveys, and time-domain electro-magnetic soundings and seismic surveys. Shaw Warm Springs appears to be the only source of thermal waters along the western side of the Valley. From the various investigations conducted the springs appear to be fault controlled and is very limited in extent. Based on best evidence presently available estimates are presented on the size and extent of Shaw Warm Springs thermal system. It is estimated that this could have an areal extent of 0.63 sq. miles (1.62 sq. km) and contain 0.0148 Q's of heat energy.

  19. Climate Projections and Drought: Verification for the Colorado River Basin

    Science.gov (United States)

    Santos, N. I.; Piechota, T. C.; Miller, W. P.; Ahmad, S.

    2017-12-01

    The Colorado River Basin has experienced the driest 17 year period (2000-2016) in over 100 years of historical record keeping. While the Colorado River reservoir system began the current drought at near 100% capacity, reservoir storage has fallen to just above 50% during the drought. Even though federal and state water agencies have worked together to mitigate the impact of the drought and have collaboratively sponsored conservation programs and drought contingency plans, the 17-years of observed data beg the question as to whether the most recent climate projections would have been able to project the current drought's severity. The objective of this study is to analyze observations and ensemble projections (e.g. temperature, precipitation, streamflow) from the CMIP3 and CMIP5 archive in the Colorado River Basin and compare metrics related to skill scores, the Palmer Drought Severity Index, and water supply sustainability index. Furthermore, a sub-ensemble of CMIP3/CMIP5 projections, developed using a teleconnection replication verification technique developed by the author, will also be compared to the observed record to assist in further validating the technique as a usable process to increase skill in climatological projections. In the end, this study will assist to better inform water resource managers about the ability of climate ensembles to project hydroclimatic variability and the appearance of decadal drought periods.

  20. Denver Radium Boom and the Colorado School of Mines

    International Nuclear Information System (INIS)

    Hart, S.S.

    1986-01-01

    The November 7, 1985, Rocky Mountain News, proclaimed Radiation hot spot detected at Mines. This hot spot discovery was the result of investigative reports by a local television station, with follow-up radiation monitoring by the Colorado Department of Health. Not an isolated occurrence of alpha and gamma radiation contamination, the School of Mines discovery is only the latest in a five-year series of discoveries of radioactive waste disposal sites in the Denver metropolitan area. These discoveries have involved not only the Colorado Department of Health, but also the Environmental Protection Agency, at least five consulting firms, and numerous businessmen and homeowners. In 1982, the sites were combined into a single project called the Denver Radium Site and selected for clean-up under the Federal Superfund program. This paper reviews the historical aspects of these hot spots by describing the history of radium processing in Denver; early work of the Colorado School of Mines, National Radium Institute, the Golden Experiment Station, and other institutional research; and the commercial production of radium. 20 references

  1. Abamectin, pymetrozine and azadirachtin sequence as a unique solution to control the leafminer Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) infesting garden beans (Phaseolus vulgaris L.) in Egypt.

    Science.gov (United States)

    Saad, A S A; Massoud, M A; Abdel-Megeed, A A M; Hamid, N A; Mourad, A K K; Barakat, A S T

    2007-01-01

    Field trails were conducted to determine the performance of three different sequences as a unique solution for the control of the leaf miner Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) infesting garden beans (Phaseolus vulgaris L.) during the two successive seasons of 2004 and 2005. Furthermore, during the evaluation period, the side effect against the ectoparasite Diglyphus isaea (Walker) (Hymenoptera: Eulophidae) was put into consideration. Meanwhile, the comparative evaluation of the pesticides alone showed that abamectin and azadirachtin were highly effective against Liriomyza trifolii, while carbosulfan, pymetrozine and thiamethoxam provided to be of a moderate effect. Moreover, carbosulfan showed harmful effect to the larvae of the ectoparasite Diglyphus isaea (Walker), while abamectin and azadirachtin gave a moderate effect. Thiamethoxam and the the detergent (Masrol 410) had slight effect in this respect. The highly effective sequence among the sequences was abamectin, pymetrozine and azadirachtin, against Liriomyza trifolii (Burgess), with slight harmful effect on Diglyphus isaea (Walker). However the sequence of azadirachtin, pymetrozine and abamectin had a moderate effect on Liriomyza trifolii (Burgess) and exhibited a slight toxic effect on Diglyphus isaea (Walker). In contrast, the sequence of carbosulfan, thiamethoxam and pymetrozine was the least effective and represented a slight effect on Diglyphus isaea (Walker). From this study, it was concluded that abamectin, pymetrozine and azadirachtin sequence has proved to be a unique solution for the control of the leaf miner Liriomyza trifolii (Burgess) infesting garden beans (Phaseolus vulgaris L.) in Egypt.

  2. Genetic diversity and population structure of an Italian landrace of runner bean (Phaseolus coccineus L.): inferences for its safeguard and on-farm conservation.

    Science.gov (United States)

    Mercati, F; Catarcione, G; Paolacci, A R; Abenavoli, M R; Sunseri, F; Ciaffi, M

    2015-08-01

    The landraces are considered important sources of valuable germplasm for breeding activities to face climatic changes as well as to satisfy the requirement of new varieties for marginal areas. Runner bean (Phaseolus coccineus L.) is one of the most cultivated Phaseolus species worldwide, but few studies have been addressed to assess the genetic diversity and structure within and among landrace populations. In the present study, 20 different populations of a runner bean landrace from Central Italy named "Fagiolone," together with 41 accessions from Italy and Mesoamerica, were evaluated by using 14 nuclear SSRs to establish its genetic structure and distinctiveness. Results indicated that "Fagiolone" landrace can be considered as a dynamic evolving open-pollinated population that shows a significant level of genetic variation, mostly detected within populations, and the presence of two main genetic groups, of which one distinguished from other Italian runner bean landraces. Results highlighted also a relevant importance of farmers' management practices able to influence the genetic structure of this landrace, in particular the seed exchanges and selection, and the past introduction in cultivation of landraces/cultivars similar to seed morphology, but genetically rather far from "Fagiolone." The most suitable on-farm strategies for seed collection, conservation and multiplication will be defined based on our results, as a model for threatened populations of other allogamous crop species. STRUCTURE and phylogenetic analyses indicated that Mesoamerican accessions and Italian landraces belong to two distinct gene pools confirming the hypothesis that Europe could be considered a secondary diversification center for P. coccineus.

  3. The Search for Braddock's Caldera-Guidebook for Colorado Scientific Society Fall 2008 Field Trip, Never Summer Mountains, Colorado

    Science.gov (United States)

    Cole, James C.; Larson, Ed; Farmer, Lang; Kellogg, Karl S.

    2008-01-01

    The report contains the illustrated guidebook that was used for the fall field trip of the Colorado Scientific Society on September 6-7, 2008. It summarizes new information about the Tertiary geologic history of the northern Front Range and the Never Summer Mountains, particularly the late Oligocene volcanic and intrusive rocks designated the Braddock Peak complex. Minor modifications were made in response to technical reviews by D.J. Lidke and C.A. Ruleman (U.S. Geological Survey) regarding clarity and consistency, and text editing by M.A. Kidd. However, the text remains essentially similar to the guidebook that was circulated to the participants on the Colorado Scientific Society 2008 field trip. Several notes were added following the trip (as indicated) to address developments since the guidebook was written.

  4. Knowledge, Norms and Preferences for Tamarisk Management in the Green and Colorado River Corridors of the Colorado Plateau

    OpenAIRE

    Allred, E. Clay

    2012-01-01

    Extensive research exists regarding invasive alien plant species including impacts to native ecosystems and efficacy of control methods on public lands and river corridors. Many studies have identified the need for more research regarding the social implications of invasive alien species management. More specifically, additional research is needed regarding the impacts of invasive alien plant management on the Colorado Plateau to river-based recreation experiences. It is important for public ...

  5. Cloud condensation nuclei in Western Colorado: Observations and model predictions

    Science.gov (United States)

    Ward, Daniel Stewart

    Variations in the warm cloud-active portion of atmospheric aerosols, or cloud condensation nuclei (CCN), have been shown to impact cloud droplet number concentration and subsequently cloud and precipitation processes. This issue carries special significance in western Colorado where a significant portion of the region's water resources is supplied by precipitation from winter season, orographic clouds, which are particularly sensitive to variations in CCN. Temporal and spatial variations in CCN in western Colorado were investigated using a combination of observations and a new method for modeling CCN. As part of the Inhibition of Snowfall by Pollution Aerosols (ISPA-III) field campaign, total particle and CCN number concentration were measured for a 24-day period in Mesa Verde National Park, climatologically upwind of the San Juan Mountains. These data were combined with CCN observations from Storm Peak Lab (SPL) in northwestern Colorado and from the King Air platform, flying north to south along the Western Slope. Altogether, the sampled aerosols were characteristic of a rural continental environment and the cloud-active portion varied slowly in time, and little in space. Estimates of the is hygroscopicity parameter indicated consistently low aerosol hygroscopicity typical of organic aerosol species. The modeling approach included the addition of prognostic CCN to the Regional Atmospheric Modeling System (RAMS). The RAMS droplet activation scheme was altered using parcel model simulations to include variations in aerosol hygroscopicity, represented by K. Analysis of the parcel model output and a supplemental sensitivity study showed that model CCN will be sensitive to changes in aerosol hygroscopicity, but only for conditions of low supersaturation or small particle sizes. Aerosol number, size distribution median radius, and hygroscopicity (represented by the K parameter) in RAMS were constrained by nudging to forecasts of these quantities from the Weather

  6. Unintentional Pediatric Exposures to Marijuana in Colorado, 2009-2015.

    Science.gov (United States)

    Wang, George Sam; Le Lait, Marie-Claire; Deakyne, Sara J; Bronstein, Alvin C; Bajaj, Lalit; Roosevelt, Genie

    2016-09-06

    As of 2015, almost half of US states allow medical marijuana, and 4 states allow recreational marijuana. To our knowledge, the effect of recreational marijuana on the pediatric population has not been evaluated. To compare the incidence of pediatric marijuana exposures evaluated at a children's hospital and regional poison center (RPC) in Colorado before and after recreational marijuana legalization and to compare population rate trends of RPC cases for marijuana exposures with the rest of the United States. Retrospective cohort study of hospital admissions and RPC cases between January 1, 2009, and December 31, 2015, at Children's Hospital Colorado, Aurora, a tertiary care children's hospital. Participants included patients 0 to 9 years of age evaluated at the hospital's emergency department, urgent care centers, or inpatient unit and RPC cases from Colorado for single-substance marijuana exposures. Marijuana. Marijuana exposure visits and RPC cases, marijuana source and type, clinical effects, scenarios, disposition, and length of stay. Eighty-one patients were evaluated at the children's hospital, and Colorado's RPC received 163 marijuana exposure cases between January 1, 2009, and December 31, 2015, for children younger than 10 years of age. The median age of children's hospital visits was 2.4 years (IQR, 1.4-3.4); 25 were girls (40%) . The median age of RPC marijuana exposures was 2 years (IQR, 1.3-4.0), and 85 patients were girls (52%). The mean rate of marijuana-related visits to the children's hospital increased from 1.2 per 100 000 population 2 years prior to legalization to 2.3 per 100,000 population 2 years after legalization (P = .02). Known marijuana products involved in the exposure included 30 infused edibles (48%). Median length of stay was 11 hours (interquartile range [IQR], 6-19) and 26 hours (IQR, 19-38) for admitted patients. Annual RPC pediatric marijuana cases increased more than 5-fold from 2009 (9) to 2015 (47). Colorado had an

  7. The 2014 Greeley, Colorado Earthquakes: Science, Industry, Regulation, and Media

    Science.gov (United States)

    Yeck, W. L.; Sheehan, A. F.; Weingarten, M.; Nakai, J.; Ge, S.

    2014-12-01

    On June 1, 2014 (UTC) a magnitude 3.2 earthquake occurred east of the town of Greeley, Colorado. The earthquake was widely felt, with reports from Boulder and Golden, over 60 miles away from the epicenter. The location of the earthquake in a region long considered aseismic but now the locus of active oil and gas production prompted the question of whether this was a natural or induced earthquake. Several classic induced seismicity cases hail from Colorado, including the Rocky Mountain Arsenal earthquakes in the 1960s and the Paradox Valley earthquakes in western Colorado. In both cases the earthquakes were linked to wastewater injection. The Greeley earthquake epicenter was close to a Class II well that had been injecting waste fluid into the deepest sedimentary formation of the Denver Basin at rates as high as 350,000 barrels/month for less than a year. The closest seismometers to the June 1 event were more than 100 km away, necessitating deployment of a local seismic network for detailed study. IRIS provided six seismometers to the University of Colorado which were deployed starting within 3 days of the mainshock. Telemetry at one site allowed for real time monitoring of the ongoing seismic sequence. Local media interest was extremely high with speculation that the earthquake was linked to the oil and gas industry. The timetable of media demand for information provided some challenges given the time needed for data collection and analysis. We adopted a policy of open data and open communication with all interested parties, and made proactive attempts to provide information to industry and regulators. After 3 weeks of data collection and analysis, the proximity and timing of the mainshock and aftershocks to the C4A injection well, along with a sharp increase in seismicity culminating in an M 2.6 aftershock, led to a decision by the Colorado Oil and Gas Corporation Commission (COGCC) to recommend a temporary halt to injection at the C4A injection well. This was the

  8. Economic impact study of the Uranium Mill Tailings Remedial Action project in Colorado: Colorado state fiscal year 1995. Revision 1

    International Nuclear Information System (INIS)

    1995-12-01

    As required by the Romer-Twining Agreement of 1990, the US Department of Energy (DOE) has prepared this annual economic impact study for the state of Colorado. This report assesses the economic impacts related to the DOE Uranium Mill Tailings Remedial Action (UMTRA) Project in Colorado during the state fiscal year (FY) between 1 July 1994 and 30 June 1995. To estimate net economic benefit, employment, salaries and wages, and other related economic benefits are discussed, quantified, and then compared to the state's 10 percent share of the remedial action costs. Actual data obtained from sites currently undergoing remedial action were used as the basis for analyses. If data were not available, estimates were used to derive economic indicators. This study describes the types of employment associated with the UMTRA Project and estimates of the numbers of people employed by UMTRA Project subcontractors in Colorado during state FY 1995. Employment totals are reported in estimated average annual jobs; however, the actual number of workers at the site fluctuates depending on weather and on the status of remedial action activities. In addition, the actual number of people employed on the Project during the year may be higher than the average annual employment reported due to the temporary nature of some of the jobs

  9. Evaluation of the texture of beans (Phaseolus vulgaris L.) of the variety carioca treated by gamma irradiation process

    International Nuclear Information System (INIS)

    Mendes, Nathalia S.R.; Silva, Yasmini P.A.; Tiraboschi, Paula C.A.; Takeuchi, Katiucha P.; Souza, Adriana R.M.; Arthur, Valter

    2011-01-01

    The bean is a staple food of the population, being one of the main products in the diet of the economically less privileged social strata. All these factors mean that beans occupy a prominent space in both the social and economic environment in Brazil [1]. In this social and economic importance of beans, adds to the growing demand, both consumers and producers, of food products that have a quality nutritional and technological properties desirable in order to obtain good quality products, which would have greater capacity competitive in the market. The quality of the beans processed depends on the growth conditions, maturity stage at harvest, processing and storage. During processing, there may be biochemical and chemical changes that affect the texture of the product [2]. Given this need, the irradiation of foods has been increasing in recent years as a preservation method that can guarantee the level of product safety, without causing major changes in nutritional and sensory characteristics of products [3].In this context, this work had the objective to evaluate the effects of irradiation on the texture of commercial beans (Phaseolus vulgaris L.) variety Carioca. The raw material (raw beans) was acquired in trade from the city of Goiania (GO) in plastic containers containing 1 kg of product. It has purchased three packs of different brands, widely accepted by local people, making a total of 3 kg of beans from each brand. It was noted the date of filling the grain, so that all the samples had approximately the same age. Thus eliminated is the age factor as a possible responsible for differences that could be observed between the samples after the time of analysis. The beans were then taken to the Laboratory of Physical Chemistry where they were removed from original containers, homogenized and packaged in polypropylene properly identified and sealed, containing 100g of product, then separated into lots. The different batches of raw beans were sent for irradiation

  10. Efecto de las condiciones de secado sobre el endurecimiento de los granos de caraota (Phaseolus vulgaris pretratados con vapor

    Directory of Open Access Journals (Sweden)

    María Virginia Mujica

    2017-12-01

    Full Text Available El endurecimiento de los granos de caraota (Phaseolus vulgaris generado por el almacenamiento en condiciones de alta temperatura y humedad relativa representa una limitante importante para su consumo. El objetivo del presente trabajo fue evaluar el efecto de un tratamiento con vapor y luego secado a los granos de P. vulgaris recién cosechados en la prevención de su endurecimiento. Inicialmente, se construyeron las curvas de secado a 40; 47,5 y 55 ºC. Seguidamente, los granos se trataron con vapor por 8 min 20 s y se sometieron a un proceso de secado, bajo un diseño central compuesto cuyos factores fueron la temperatura del aire (40 y 55 ºC y el tiempo de secado (4 y 8 h. La muestra secada de cada tratamiento se dividió en 3 lotes, el primero se analizó de inmediato y los otros se almacenaron por 5 semanas bajo 2 condiciones distintas 5 ºC/34 % HR y 37 ºC/75 % HR. Las determinaciones realizadas fueron humedad, capacidad de imbibición, tiempo de cocción y actividad de la peroxidasa soluble. El tiempo de secado fue de 6,5; 5 y 2,5 h a 40; 47,5 y 55 ºC, respectivamente, para alcanzar una humedad de 13 % (b.s.. El coeficiente de difusión resultó igual a 3,40x10-9; 3,52x10-9 y 3,87x10-9 m2/s, a 40, 47,5 y 55 ºC, respectivamente. El valor de la energía de activación fue de 7,33 kJ/mol. La aplicación de vapor y posterior secado de los granos no previno su endurecimiento, y este no dependió de la actividad de la peroxidasa soluble. Por lo que se puede inferir que los compuestos fenólicos y la enzima peroxidasa no son los únicos implicados en el mecanismo de endurecimiento de los granos de P. vulgaris.

  11. Evaluation of the texture of beans (Phaseolus vulgaris L.) of the variety carioca treated by gamma irradiation process

    Energy Technology Data Exchange (ETDEWEB)

    Mendes, Nathalia S.R.; Silva, Yasmini P.A.; Tiraboschi, Paula C.A.; Takeuchi, Katiucha P.; Souza, Adriana R.M., E-mail: adriana.souza@pesquisador.cnpq.br [Escola de Agronomia e Engenharia de Alimentos. Universidade Federal de Goias - UFG, Goiania, GO (Brazil); Arthur, Valter, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2011-07-01

    The bean is a staple food of the population, being one of the main products in the diet of the economically less privileged social strata. All these factors mean that beans occupy a prominent space in both the social and economic environment in Brazil [1]. In this social and economic importance of beans, adds to the growing demand, both consumers and producers, of food products that have a quality nutritional and technological properties desirable in order to obtain good quality products, which would have greater capacity competitive in the market. The quality of the beans processed depends on the growth conditions, maturity stage at harvest, processing and storage. During processing, there may be biochemical and chemical changes that affect the texture of the product [2]. Given this need, the irradiation of foods has been increasing in recent years as a preservation method that can guarantee the level of product safety, without causing major changes in nutritional and sensory characteristics of products [3].In this context, this work had the objective to evaluate the effects of irradiation on the texture of commercial beans (Phaseolus vulgaris L.) variety Carioca. The raw material (raw beans) was acquired in trade from the city of Goiania (GO) in plastic containers containing 1 kg of product. It has purchased three packs of different brands, widely accepted by local people, making a total of 3 kg of beans from each brand. It was noted the date of filling the grain, so that all the samples had approximately the same age. Thus eliminated is the age factor as a possible responsible for differences that could be observed between the samples after the time of analysis. The beans were then taken to the Laboratory of Physical Chemistry where they were removed from original containers, homogenized and packaged in polypropylene properly identified and sealed, containing 100g of product, then separated into lots. The different batches of raw beans were sent for irradiation

  12. Coefficients of leaf-fruit translocation for 60Co, 90Sr and 137Cs in bean plant (Phaseolus vulgaris)

    International Nuclear Information System (INIS)

    Macacini, Jose Flavio

    2000-01-01

    Due to the increasing use of nuclear fission for the generation of electrical energy, the safety aspects of power plants must be minutely appraised. In case of an accident, with liberation of radioactive material into the atmosphere, knowledge about the behavior of plant species when in contact with radionuclides is indispensable. An important route through which agricultural products are contaminated by radionuclides is leaf-fruit translocation. This phenomenon can be evaluated by simulating a fallout contamination in a controlled atmosphere using as a tracer man-made radionuclides. In order to quantity the leaf-fruit translocation coefficients for 60 Co, 90 Sr and 137 Cs in the common bean (Phaseolus vulgaris), variety black diamond, an experiment was carried out in a greenhouse with completely randomized blocks design with six treatments and four blocks. A mixture of these three radionuclides was prepared and used to determine their translocation coefficients. The bean plants were contaminated inside a device especially designed to avoid environmental contamination. In each treatment four vases were sprinkled and one was used to estimate the initial activity of the other three vases. High-resolution gamma-ray spectrometry was used for 60 Co and 137 Cs activity determinations and chemical separation followed by beta counting of 90 Y was used for 90 Sr determinations. The number of treatments was reduced from six to four sprayings corresponding to 30, 45, 60 and 75 days after planting. This reduction was due to the attack of common and gold mosaic viroses. Symptoms were observed on the diseased bean plants 50 days after planting. It was possible, however, to verify a functional dependence between instant of tracer application and the level of physiological development of the bean plant. It was verified that the temporal relationship values for leaf-fruit translocation were similar for 60 Co and 137 Cs. For the 90 Sr, the translocation was below 2,5 mBq kg -1 /Bq

  13. Evaluation of seed yield and competition indices of corn (Zea mays L. intercropped with different bean (Phaseolus spp. types

    Directory of Open Access Journals (Sweden)

    Hakime Ziaei

    2016-05-01

    Full Text Available In order to evaluate the intercropping of corn (Zea mays L. and bean cultivars (Phaseolus spp. an experiment was carried out in a randomized complete block design with three replicaties at Sari Agricultural Sciences and Natural Resources University during growing season of 2010. The experimental treatments consisted of sole cropping of corn, white bean, bush bean, red bean, pinto bean and sword bean and 50:50 ratio of corn and bean types. In this experiment, the corn-bush bean and corn-pinto bean intercropping had the highest seed yield (5734.4 and 5674.3 kg/ha-1, respectively and land equivalent ratio (LER=1.13 and 1.21, respectively. Evaluated intercropping indices indicated that red bean (k= 1.85, pinto bean (k= 2.41 and sword bean (k= 2.80 had the highest crowding coefficient whereas the maximum aggressivity value was belonged to pinto bean intercropped with corn (A= -0.02. Also, both the red bean and pinto bean (CR=0.75 and CR=0.98, respectively had the maximum competitive ratio. Furthermore, the most corn crowding coefficient (K=1.15 was belonged to corn and sword bean intercropping and maximum corn aggressivity value was observed in corn intercropped with white bean (A=+0.60 and bush bean (A=+0.69. In conclusion, according to competition indices, intercropping of 50% corn + 50 % red bean and pinto bean plants were superior as compared to other combinations.Also, both the red bean and pinto bean (CR=0.75 and CR=0.98, respectively had the maximum competitive ratio. Furthermore, the most corn crowding coefficient (K=1.15 was belonged to corn and sword bean intercropping and maximum corn aggressivity value was observed in corn intercropped with white bean (A=+0.60 and bush bean (A=+0.69. In conclusion, according to competition indices, intercropping of 50% corn + 50 % red bean and pinto bean plants were superior as compared to other combinations.

  14. Do French beans (Phaseolus vulgaris L. grown in proximity to Mt Kenya forest- Kenya- experience pollination deficit?

    Directory of Open Access Journals (Sweden)

    Rose Masiga

    2014-09-01

    Full Text Available Yields of commercially important crops in Kenya are often far below their potential. Amongst the possible reasons for such low yields may be the ecosystem degradation that can be expected to have negative impacts on pollinator presence in cropland, and the consequent food security issue for smallholder farmers who depend on these crops for their livelihood. Our study was carried out to assess the potential pollination deficit of French beans (Phaseolus vulgaris L., a major export vegetable crop in Kenya grown by small-scale farmers. Sufficient pollination of French beans likely results in high seed set and uniform heavier green pods. Such pods get the highest grade while malformed pods are unmarketable, reducing family income. We hypothesized that pollination success was linked to the abundance and diversity of large pollinators, itself associated with the proximity to natural habitats. Flower visitors to French beans were sampled in 2011 and 2012 in ten farmer-managed plots, five within 200 m from the edge of Mt. Kenya forest and five farther away, more than 1000 m. Each plot measured 760 m2 and was planted at the same time, with the “Julia” variety. Flowers were observed for 2 h in each plot once weekly for three weeks at peak flowering from 0900-1100 h in the morning and 1200 – 1400 h in the afternoon on alternate days. Honey bees (Apis mellifera were the most abundant visitors of French bean flowers followed by carpenter bees (Xylocopa spp. and leafcutter bees (Megachile spp.. Significantly higher numbers of leafcutter bees were recorded on farms far to the forest. There was no significant difference in honey bee abundance among the study sites, probably because apiaries and wild colonies are located across the landscape. French bean yield was significantly correlated with the mean abundance of carpenter bees in 2011. This suggests the possible occurrence of pollination deficit in French beans where the density of carpenter bees is

  15. Pencegahan hipertensi dan penebalan dinding aorta dengan pemberian kecambah kacang hijau (Phaseolus radiatus (L pada tikus putih Sprague Dawley

    Directory of Open Access Journals (Sweden)

    Novian Swasono Hadi

    2016-01-01

    Full Text Available Background: Lifestyle changes with high-fat food consumption is one of the factors the risks of cardiovascular diseases like of coronary heart disease and atherosclerosis. A healthy diet and a balanced diet and consume foods that contain lots of antioxidants is one of the effective ways to prevent hyperlipidemia. Mung bean sprouts have properties that neutralize free radicals cause Hyperlipidemia and cardiovascular diseases because it is an antioxidant compound. Objective: The aim of this study was to determinate the effect of mung bean sprouts (Phaseolus radiatus (L to blood pressure and histopathology aorta of Sprague-Dawley male rats. Method: The type of study was experimental research using pre-post test controlled group design for blood pressure variable and post test only controlled group design histopathology aorta. The thirty-five of Sprague-Dawley male rats was eight weeks divided into 5 groups. The first group was given standard diet, group 2 was given a hight fat diet, the third group was given a high-fat diet and mung bean sprout 0,67 gram, group 4 was given a high-fat diet and mung bean 1,34 gram, and group 5 was given a high-fat diet and vitamin E doses of 23 IU. Results: Result of this study showed that after 4 weeks of treatment, increased in blood pressure systole in the given of  high fat diet higher than group who were given a high fat diet and mung bean sprout and also on group who were given high fat diet and vitamin E, but there is no difference effect a decrease in blood pressure between the provision of mung bean sprouts and vitamin E (p>0,05. Statistical analysis to thick the wall the aorta show the similarity meaningful in all the treatment group, it can be said that overall thick the wall the aorta in this research is not different. Conclusion: A dose of mung bean sprout 0,67 g is optimal doses in preventing a rise in blood pressure and prevent alterations histopathology Sprague-Dawley male rats.

  16. The Colorado Front Range Ecosystem Management Research Project: Accomplishments to date

    Science.gov (United States)

    Brian Kent; Wayne D. Shepperd; Deborah J. Shields

    2000-01-01

    This article briefly describes the goals and objectives for the Colorado Front Range Ecosystem Management Project (FREM). Research under this project has addressed both biophysical and human dimensions problems relating to ecosystem management in the Colorado Front Range. Results of completed work are described, and the status of the ongoing demonstration project at...

  17. 76 FR 5432 - United Western Bank Denver, Colorado; Notice of Appointment of Receiver

    Science.gov (United States)

    2011-01-31

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision United Western Bank Denver, Colorado... section 5(d)(2) of the Home Owners' Loan Act, the Office of Thrift Supervision has duly appointed the Federal Deposit Insurance Corporation as sole Receiver for United Western Bank, Denver, Colorado, (OTS No...

  18. A Collaborative Approach to Diabetes Management: The Choice Made for Colorado Schools

    Science.gov (United States)

    Bobo, Nichole; Wyckoff, Leah; Patrick, Kathleen; White, Cathy; Glass, Sue; Carlson, Jessie Parker; Perreault, Christine

    2011-01-01

    Students with diabetes deserve a school nurse who can effectively manage the disease. Tensions between the school and families sometimes emerge when a child with diabetes goes to school. To resolve these tensions in Colorado, stakeholders collaborated to implement a statewide program to meet the needs of students with diabetes. Colorado school…

  19. 77 FR 13629 - Notice of Inventory Completion: History Colorado, Denver, CO

    Science.gov (United States)

    2012-03-07

    ... Fort Hall Reservation of Idaho; and the Zuni Tribe of the Zuni Reservation, New Mexico. History and...: History Colorado, Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: History... human remains may contact History Colorado. Disposition of the human remains to the Indian tribes stated...

  20. Literacy in a Bag: Colorado School for the Deaf Sends Reading Home

    Science.gov (United States)

    Branch, Deborah

    2009-01-01

    To assist parents with deaf or hard of hearing children who may need help supporting their child's learning, the Colorado School for the Deaf and the Blind developed Family Literacy Packs. These literacy packs, available to families within the state of Colorado, provide fun, interactive activities that help parents support their children's…

  1. Community-based restoration of desert wetlands: the case of the Colorado River delta

    Science.gov (United States)

    Osvel Hinojosa-Huerta; Mark Briggs; Yamilett Carrillo-Guerroro; Edward P. Glenn; Miriam Lara-Flores; Martha Roman-Rodriguez

    2005-01-01

    Wetland areas have been drastically reduced through the Pacific Flyway and the Sonoran Desert, with severe consequences for avian populations. In the Colorado River delta, wetlands have been reduced by 80 percent due to water management practices in the Colorado River basin. However, excess flows and agricultural drainage water has restored some areas, providing...

  2. 77 FR 21451 - Approval and Promulgation of Air Quality Implementation Plans; Colorado; Procedural Rules...

    Science.gov (United States)

    2012-04-10

    ... Promulgation of Air Quality Implementation Plans; Colorado; Procedural Rules; Conflicts of Interest AGENCY... potential conflicts of interest. We are also approving the remaining portion of Colorado's January 7, 2008... or more Indian tribes, on the relationship between the Federal Government and Indian tribes, or on...

  3. Metadata, Cataloging, Digitization and Retrieval--Who's Doing What to Whom: The Colorado Digitization Project Experience.

    Science.gov (United States)

    Bishoff, Liz; Garrison, William A.

    This paper describes the experiences of the Colorado Digitization Project (CDP) related to accessing a diverse set of primary resources held by many different cultural heritage institutions. The CDP is a collaborative initiative involving Colorado's archives, historical societies, libraries, and museums. The CDP's goal is to create a virtual…

  4. 76 FR 70170 - Proposed Alternative Soils Standards for the Uravan, Colorado Uranium Mill

    Science.gov (United States)

    2011-11-10

    ..., Colorado Uranium Mill AGENCY: Nuclear Regulatory Commission. ACTION: Uranium milling alternative standards... Agreements to regulate uranium mill tailings (11e.(2) byproduct material). Six Agreement States have this... in Colorado are acceptable. Discussion The Uravan site began operations in 1912 as a radium mill and...

  5. 77 FR 21760 - Atmos Energy Colorado/Kansas Division; Notice of Baseline Filing

    Science.gov (United States)

    2012-04-11

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR12-19-000] Atmos Energy Colorado/Kansas Division; Notice of Baseline Filing Take notice that on March 30, 2012, Atmos Energy Colorado/Kansas Division (Atmos) submitted a baseline filing of their Statement of Operating Conditions for...

  6. 77 FR 23244 - Atmos Energy Colorado/Kansas Division; Notice of Revised Baseline Filing

    Science.gov (United States)

    2012-04-18

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR12-22-000] Atmos Energy Colorado/Kansas Division; Notice of Revised Baseline Filing Take notice that on April 10, 2012, Atmos Energy Colorado/Kansas Division (Atmos) filed a revised baseline filing of their Statement of Operating...

  7. Casa de la Esperanza: A Case Study of Service Coordination at Work in Colorado.

    Science.gov (United States)

    Franquiz, Maria E.; Hernandez, Carlota Loya

    This chapter describes how a federally funded farmworker housing facility in northern Colorado--Casa de la Esperanza--has changed the lives of migrant students and their families. The history of migrant workers in Colorado is described, as well as the struggle to construct a permanent farmworker housing facility. Casa was built in Boulder County,…

  8. Colorado Multicultural Resources for Arts Education: Dance, Music, Theatre, Visual Arts, and Folk Arts. Second Edition.

    Science.gov (United States)

    Kitzelman, Jacquie, Ed.

    This resource guide recognizes that the arts provide a natural arena for teaching multicultural perspectives to students of all ages. The guide features the four most prevalent ethnic minorities in Colorado, African American, Asian American, Hispanic American, and Native American, plus a section prepared by Colorado's state folklorists. The…

  9. Colorado Multicultural Resources for Arts Education: Dance, Music, Theatre, and Visual Art.

    Science.gov (United States)

    Cassio, Charles J., Ed.

    This Colorado resource guide is based on the premise that the arts (dance, music, theatre, and visual art) provide a natural arena for teaching multiculturalism to students of all ages. The guide provides information to Colorado schools about printed, disc, video, and audio tape visual prints, as well as about individuals and organizations that…

  10. Advancing the "Colorado Graduates" Agenda: Understanding the Dropout Problem and Mobilizing to Meet the Graduation Challenge

    Science.gov (United States)

    Mac Iver, Martha Abele; Balfanz, Robert; Byrnes, Vaughan

    2009-01-01

    The ambitious goal set by Colorado's governor to address the state's dropout problem is a model for the nation. Helping thousands of young people to receive their high school diplomas instead of leaving school without them is a crucial step in improving the quality of life for all Colorado residents. Accomplishing this goal will require focused…

  11. Guia del Proceso del IFSP de Colorado: Conexiones para la Ninez Temprana, Iniciativa Infantil de Colorado Parte C del Acta de Educacion para Individuos con Desabilidades (Colorado Guidelines for the IFSP Process: Early Childhood Connections, Colorado's Infant/Toddler Initiative for Part C of the Individuals with Disabilities Education Act).

    Science.gov (United States)

    Miller, Jerri; Petersen, Sandy

    This booklet for Spanish-speaking parents of young children with disabilities describes Colorado's Individualized Family Service Plan (IFSP) process. It explains guidelines, shares family stories and reflections for families and care providers, and the describes the values that drive the IFSP process in Colorado. Information is provided on…

  12. 30 CFR 906.15 - Approval of Colorado regulatory program amendments.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Approval of Colorado regulatory program amendments. 906.15 Section 906.15 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE COLORADO...

  13. 78 FR 44186 - Colorado Disaster # CO-00058 Declaration of Economic Injury

    Science.gov (United States)

    2013-07-23

    ... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13662] Colorado Disaster CO-00058 Declaration... notice of an Economic Injury Disaster Loan (EIDL) declaration for the State of Colorado, dated 07/15/2013... areas have been determined to be adversely affected by the disaster: Primary Counties: Hinsdale, Mineral...

  14. 76 FR 53693 - Notice of Invitation To Participate; Coal Exploration License Application COC-74911, Colorado

    Science.gov (United States)

    2011-08-29

    ...] Notice of Invitation To Participate; Coal Exploration License Application COC-74911, Colorado AGENCY: Bureau of Land Management, Interior. ACTION: Notice. SUMMARY: Pursuant to the Mineral Leasing Act of 1920... America in lands located in Delta County, Colorado. DATES: This notice of invitation was published in the...

  15. 30 CFR 906.20 - Approval of Colorado abandoned mine land reclamation plan.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Approval of Colorado abandoned mine land reclamation plan. 906.20 Section 906.20 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE COLORADO...

  16. 78 FR 50088 - Notice of Availability of the Northwest Colorado Greater Sage-Grouse Draft Resource Management...

    Science.gov (United States)

    2013-08-16

    ... Availability of the Northwest Colorado Greater Sage- Grouse Draft Resource Management Plan Amendment and Draft... Land Management (BLM) has prepared a Northwest Colorado Greater Sage-Grouse Draft Resource Management.../or mailings. ADDRESSES: You may submit comments related to the Northwest Colorado Greater Sage-Grouse...

  17. 75 FR 17034 - Irish Potatoes Grown in Colorado; Relaxation of the Handling Regulation for Area No. 3

    Science.gov (United States)

    2010-04-05

    ...; FV09-948-2 IFR] Irish Potatoes Grown in Colorado; Relaxation of the Handling Regulation for Area No. 3...: This rule relaxes the size requirement prescribed under the Colorado potato marketing order. The marketing order regulates the handling of Irish potatoes grown in Colorado, and is administered locally by...

  18. 78 FR 3 - Irish Potatoes Grown in Colorado; Modification of the Handling Regulation for Area No. 2

    Science.gov (United States)

    2013-01-02

    ...; FV12-948-1 IR] Irish Potatoes Grown in Colorado; Modification of the Handling Regulation for Area No. 2...: This rule modifies the grade requirements for potatoes handled under the Colorado potato marketing order, Area No. 2 (order). The order regulates the handling of Irish potatoes grown in Colorado and is...

  19. 78 FR 35743 - Irish Potatoes Grown in Colorado; Modification of the General Cull and Handling Regulation for...

    Science.gov (United States)

    2013-06-14

    ... IR] Irish Potatoes Grown in Colorado; Modification of the General Cull and Handling Regulation for.... SUMMARY: This interim rule modifies the size requirements for potatoes handled under the Colorado potato marketing order, Area No. 2 (order). The order regulates the handling of Irish potatoes grown in Colorado...

  20. 2014 Kids Count in Colorado! The Big Picture: Taking the Whole Child Approach to Child Well-Being

    Science.gov (United States)

    Colorado Children's Campaign, 2014

    2014-01-01

    "Kids Count in Colorado!" is an annual publication of the Colorado Children's Campaign, which provides the best available state- and county-level data to measure and track the education, health and general well-being of the state's children. "Kids Count in Colorado!" informs policy debates and community discussions, serving as…