WorldWideScience

Sample records for frijol colorado phaseolus

  1. Ocurrencia de tóxicos naturales en frijol colorado (phaseolus vulgaris) y arveja (pisum sativum). efecto del tiempo de almacenamiento y los tratamientos caseros.

    OpenAIRE

    2011-01-01

    Las leguminosas, consideradas como una de los principales alimentos para el hombre pueden contener diferentes sustancias conocidas como antinutrientes las cuales tienen cierto efecto en la nutrición humana y animal si no son removidos o inactivados adecuadamente. En Cuba existe muy poca información sobre cuales son los antinutrientes y en qué concentraciones se encuentran en dos de las leguminosas de importación de mayor consumo por nuestra población: el frijol común (Phaseolus vulgaris) y la...

  2. Ocurrencia de tóxicos naturales en frijol colorado (Phaseolus vulgaris y arveja (Pisum sativum. Efecto del tiempo de almacenamiento y los tratamientos caseros.

    Directory of Open Access Journals (Sweden)

    Bilbao Reboredo Tania

    2000-06-01

    Full Text Available Las leguminosas, consideradas como una de los principales alimentos para el hombre pueden contener diferentes sustancias conocidas como antinutrientes las cuales tienen cierto efecto en la nutrición humana y animal si no son removidos o inactivados adecuadamente. En Cuba existe muy poca información sobre cuales son los antinutrientes y en qué concentraciones se encuentran en dos de las leguminosas de importación de mayor consumo por nuestra población: el frijol común (Phaseolus vulgaris y la arveja (Pisum sativum y tampoco se han estudiado los cambios que pueden sufrir durante su almacenamiento, así como la efectividad de los tratamientos caseros. Muestras de estos granos fueron analizadas durante un año de almacenamiento comercial a una temperatura promedio de 27 °C y 79 % de humedad relativa. Se evaluó la presencia de taninos, ácido cianhídrico, ácido fítico, inhibidores de proteasas y lectinas, en los granos crudos y después de dos tratamientos: remojo y cocción y cocción directamente, en olla de presión. Todos los antinutientes disminuyeron con el tiempo de almacenamiento (p<0,05. Los tratamientos aplicados eliminaron el ácido cianhídrico, los inhibidores de tripsina y las lectinas en los dos tipos de granos, comprobándose la efectividad de la combinación remojo-cocción sobre la aplicación únicamente del tratamiento térmico, sobre el resto de los antinutrientes.

  3. Digestibilidad del almidón en diferentes variedades de frijol (Phaseolus Vulgaris L.)

    OpenAIRE

    Apolonio Vargas-Torres; Perla Osorio-Díaz; Edith Agama-Acevedo; Leticia Morales-Franco; Luis Arturo Bello-Pérez

    2006-01-01

    Se estudió la composición química y la digestibilidad del almidón de cuatro variedades de frijol común (Phaseolus vulgaris L.). No se encontraron diferencias estadísticas (p¿0,05) en el contenido de proteína y el contenido de lípidos fue bajo pero sin diferencias apreciables. Estos frijoles son buena fuente de minerales (3,67-5,15% cenizas). El valor de almidón total (AT) menor fue para la variedad Flor de Mayo (34,70%) y el mayor para Peruano (42,81%) y Mayocoba (40,24%). Los valores mayores...

  4. Genotipos de frijol (Phaseolus Vulgaris l. resistentes a Xanthomonas campestris pv. phaseoli de Mexico

    Directory of Open Access Journals (Sweden)

    Rosa Navarrete

    2000-01-01

    Full Text Available Genotipos de frijol (Phaseolus vulgaris L. resistentes a Xanthomonas campestris pv. phaseoli de México. Durante 1995 se evaluó la reacción de genotipos de frijol de diversos origenes a Xcp, bajo condiciones de invernadero en el Campo Experimental del Valle de México, del INIFAP. Se realizaron tres experimentos con a120, b44 y csiete genotipos de frijol. Las plantas se inocularon por corte con navajas en la etapa V3, a y b con una mezcla de nueve cepas de Xcp y el c, con cada una de siete cepas con diferente grado de patogenicidad. La severidad se evaluó 20 días después de la inoculación, por comparación con una escala visual de nueve grados. Los datos se analizaron bajo un diseño completamente al azar. En a, los genotipos que mostraron reacción de resistencia a Xcp fueron: A 36, A 475, G 5686, G 11867, Harowood, SEA 14, XAN 266, MCD 4012 y REN 27. En b los genotipos resistentes fueron: Sequía Durango, Taylor y XAN 30. En los experimentos anteriores la severidad de la enfermedad mostró una distribución normal, con el máximo número de genotipos en el grado de severidad cinco en a y seis en b. Los resultados obtenidos indican que el uso de mezclas de cepas de bacterias con diferente patogenicidad es eficiente para identificar genotipos de frijol resistentes a Xcp. Los genotipos resistentes identificados en el último experimento, mostraron respuesta diferencial e interacciones genotipo por cepa. REN 27 y SEA 14 mostraron resistencia a las cepas utilizadas

  5. Los frijoles (Phaseolus Vulgaris: su aporte a la dieta del costarricense

    Directory of Open Access Journals (Sweden)

    Ligia Rodríguez-Castillo

    2003-09-01

    Full Text Available Los frijoles constituyen un alimento básico en la dicta del costarricense. Sin embargo, la cantidad y frecuencia con que se consumen ha venido disminuyendo, sobretodo en la zona urbana. Esta tendencia resulta preocupante, ya que el frijol es fuente de proteínas, hierro vegetal, fibra, ácido fólico, tiamina, potasio, magnesio, y zinc (USDA, 2000. Su aporte a la dieta del costarricense, a pesar de la disminución en su consumo, es importante por su contenido de nutrientes y de fitoquímicos los cuales son de utilidad en la prevención y tratamiento de varias de las patologías que afectan a la población costarricense. La presente revisión, documenta los efectos positivos que algunos nutrientes y otros elementos que aporta el frijol tienen en la prevención y el control de algunas de las enfermedades crónicas que constituyen problemas de salud pública en el país. Se abordan de manera particular la fibra, el ácido fólico y el magnesio. Asimismo, se hace referencia a otras sustancias fitoquímicas, presentes en los frijoles, que constituyen elementos protectores de la salud y que hacen de los frijoles un "alimento funcional". Finalmente, se ofrecen recomendaciones prácticas orientadas a mejorar la digestibilidad de este alimento, de manera que se contribuya a mantener e incrementar su consumo a nivel de los hogares costarricenses.Beans (Phaseolus vulgaris are an important part of the Costa Rican diet. Nevertheless, the amount and the frequency of its consumption has been decreasing over time, especially in the urban areas. This tendency is a concern to those involved in health and nutrition since beans are a source of protein, non-heme iron, fiber, folic acid, thiamin, potassium , magnesium and zinc. The nutritional contribution of beans lo the Costa Rican diet is important in spite of the reduction in consumption. Beans are important because of their nutrient content and the presence of phytochemicals that have been recognized as part of

  6. Cartografía de QTL asociados a la tolerancia a estrés hídrico en frijol común (Phaseolus vulgaris L.)

    OpenAIRE

    Villordo Pineda, Emiliano

    2015-01-01

    Phaseolus vulgaris L. (frijol común o judía) es una leguminosa de gran demanda para la nutrición humana y un producto agrícola muy importante. Sin embargo, la producción de frijol se ve limitada por presiones ambientales como la sequía. En México, el 85% de la cosecha de frijol se produce en la temporada de primavera-verano, principalmente en las regiones del altiplano semiárido con una precipitación anual entre 250 y 400 mm. A pesar del implemento de tecnología en el campo, los factores natu...

  7. Fraccionamiento de nitrógeno en frijol ( phaseolus vulgaris l. en el valle de san juan

    Directory of Open Access Journals (Sweden)

    Juan Cedano

    2000-01-01

    Full Text Available Fraccionamiento de nitrógeno en frijol (Phaseolus vulgaris L. en el valle de San Juan. Se realizó un estudio para determinar el efecto del fraccionamiento de la fertilización nitrogenada y el momento adecuado para la aplicación de nitrógeno en el cultivo de frijol (Phaseolus vulgaris L. en cinco localidades del Valle de San Juan, R. D. Los experimentos fueron establecidos del 5 al 14 de noviembre (1997 , se utilizó un diseño de bloques completamente al azar y nueve tratamientos en cada localidad, encontrándose que en los terrenos con altos niveles de nitrógeno no hubo respuesta a la aplicación de nitrógeno ni al fraccionamiento de este nutrimento; mientras que en los suelos con deficiencia en nitrógeno si hubo respuesta a la fertilización nitrogenada encontrándose diferencia estadística significativa a la aplicación y al momento de aplicación del fertilizante. Entre las localidades hubo diferencia estadística significativa (P>0.05, mientras que no se encontró interacción entre los tratamientos y las localidades

  8. EVALUACIÓN DEL IMPACTO DE LA SELECCIÓN PARTICIPATIVA DE VARIEDADES DE FRIJOL (Phaseolus vulgaris L.) EN LA PALMA, PINAR DEL RÍO

    OpenAIRE

    2005-01-01

    El presente trabajo se realizó en el municipio La Palma, provincia Pinar del Río, Cuba, para evaluar el impacto del fitomejoramiento participativo sobre el sistema local de semillas de los pequeños agricultores del frijol común (Phaseolus vulgaris L.). El trabajo de investigación consideró realizar el primer ejercicio de selección participativa de variedades de frijol en una feria de diversidad efectuada en una comunidad rural en Cuba, la cual fue organizada por un agricultor en la localidad ...

  9. Estrategia para el manejo de suelos ácidos en frijol (phaseolus vulgaris l. en el estado de Chiapas, Mexico

    Directory of Open Access Journals (Sweden)

    Bernardo Villar S\\u00E1nchez

    2000-01-01

    Full Text Available Estrategia para el manejo de suelos ácidos en frijol (Phaseolus vulgaris L. en el estado de Chiapas, México. Por su contribución en la dieta alimenticia y la generación de empleos, y por existir una ancestral cultura productiva y una superficie de siembra de más de 100 mil hectáreas, el cultivo de frijol es de fundamental importancia en el estado de Chiapas. Sin embargo, se ha determinado una brecha tecnológica de más de 700 kg/ha entre el potencial del cultivo y los rendimientos actuales. Esta es ocasionada por numerosos factores limitantes entre los que destaca la presencia de suelos de baja fertilidad y ácidos. La estrategia adoptada para el manejo de este problema incluye: 1.Aplicación de cal y fósforo; 2. Mejoramiento genético para resistencia; 3.Manejo de los ciclos de materia orgánica y nutrientes del suelo; y 4.Combinación de las tres alternativas. En este reporte se dan los avances obtenidos con relación a la primera alternativa considerada como el paso inicial para el logro de un manejo integral de suelos en Chiapas. Durante 1997 se estudiaron cinco dosis de cal y tres de fósforo en condiciones de invernadero y campo para suelos de diferentes localidades. Se utilizó un experimento factorial completo con cuatro repeticiones, los experimentos fueron evaluados en términos de la producción de grano y al efecto de la cal sobre las propiedades químicas del suelo. Hubo respuesta del frijol en rendimiento a la aplicación de cal y fósforo

  10. EVALUACIÓN SENSORIAL DE FRIJOL (Phaseolus vulgaris L. MEJORADO NUTRICIONALMENTE EN DOS COMUNIDADES CUBANAS

    Directory of Open Access Journals (Sweden)

    Robert Manuel Leyva-Mart\\u00EDnez

    2010-01-01

    Full Text Available El objetivo del presente trabajo fue evaluar sensorialmente el frijol común mejorado nutricionalmente (línea 38 y la variedad local (BAT 304 con productores y consumidores de dos comunidades de Holguín, Cuba. Se aplicó una encuesta exploratoria que determinó el alto consumo de los frijoles negros pequeños, y las recetas: arroz con frijoles y potaje. Se seleccionó una muestra de 80 personas y se aplicaron tres pruebas entre marzo y junio del 2008: discriminatoria triangular, aceptabilidad y preferencia. Se encontró que 50 personas discriminaron correctamente entre las dos variedades (P<0,000001. No hubo diferencia significativa (P=0,9 en el porcentaje de participantes que clasificaron (en una escala de uno a cuatro la consistencia del caldo, el sabor del potaje y la textura del grano entre las variedades; sin embargo, al calificar estas características con palabras, notaron una diferencia en consistencia y textura, mas no en el sabor del potaje elaborado con los dos tipos de frijol. Finalmente, siete evaluadores no indicaron una preferencia por el potaje elaborado con uno u otro tipo de frijol. De los 73 que sí expresaron una preferencia, no manifestaron diferencia estadísticamente importante (P=0,13. En conclusión, existió diferencia sensorial entre los frijoles, pero la preferencia fue similar, lo que sugiere una buena aceptación del consumidor por el frijol mejorado nutricionalmente.

  11. Contenido de aflatoxinas y proteína en 13 variedades de frijol (Phaseolus vulgaris L. Aflatoxin and protein content in 13 bean (Phaseolus vulgaris L. varieties

    Directory of Open Access Journals (Sweden)

    Silvia Denise Peña-Betancourt

    2012-02-01

    Full Text Available En México el frijol (Phaseolus vulgaris L. es una semilla leguminosa de elevado consumo (11 kg per cápita anualmente, por lo que su cultivo es amplio en diferentes regiones. En este estudió se determinó la presencia de aflatoxinas en ocho variedades de frijol común y cinco variedades de frijol mejorado; además del contenido de proteína y humedad. En todas las variedades evaluadas el contenido de humedad mostró grandes variaciones (6 a 16%, encontrándose 16% de las variedades estudiadas fuera de la normatividad (In Mexico, the bean (Phaseolus vulgaris L. is a highly consumed legume seed (11 kg per capita annually, so that its cultivation it' s quite extensive in different regions. In this study we investigated the presence of aflatoxins in eight common bean varieties and five improved bean varieties, in addition to protein and moisture. In all the varieties tested, the moisture content showed large variations (from 6 to 16%, being 16%of the varieties studied outside the normal (< 12%. The highest content of protein was detected in the improved bean varieties (26.1% and, the lowest in commercial (19.8% ± 3.09 and wild varieties (20.78% ± 1.93. All of them showed aflatoxins on average of 7.46 ng g-¹ and a range from 5 to 13 ng g-¹. The highest level of contamination was observed in the improved bean varieties (9.2 ±2.9 ngg-¹, followed by the commercial ones ±0.95 7.25 ngg-¹ and wild varieties 6 ± 1 ng g-¹. Tannins were detected in wild bean varieties at a level of 0.44%± 0.13. The results obtained confirm the presence of toxic and anti-nutritional compounds in the different varieties of common and wild beans at levels permitted by national law, but may pose a risk to the consumer's health due to its high consumption.

  12. Los frijoles (Phaseolus Vulgaris): su aporte a la dieta del costarricense

    OpenAIRE

    2003-01-01

    Los frijoles constituyen un alimento básico en la dicta del costarricense. Sin embargo, la cantidad y frecuencia con que se consumen ha venido disminuyendo, sobretodo en la zona urbana. Esta tendencia resulta preocupante, ya que el frijol es fuente de proteínas, hierro vegetal, fibra, ácido fólico, tiamina, potasio, magnesio, y zinc (USDA, 2000). Su aporte a la dieta del costarricense, a pesar de la disminución en su consumo, es importante por su contenido de nutrientes y de fitoquímicos los ...

  13. Los frijoles (Phaseolus Vulgaris: su aporte a la dieta del costarricense

    Directory of Open Access Journals (Sweden)

    Ligia Rodríguez-Castillo

    2003-09-01

    Full Text Available Los frijoles constituyen un alimento básico en la dicta del costarricense. Sin embargo, la cantidad y frecuencia con que se consumen ha venido disminuyendo, sobretodo en la zona urbana. Esta tendencia resulta preocupante, ya que el frijol es fuente de proteínas, hierro vegetal, fibra, ácido fólico, tiamina, potasio, magnesio, y zinc (USDA, 2000. Su aporte a la dieta del costarricense, a pesar de la disminución en su consumo, es importante por su contenido de nutrientes y de fitoquímicos los cuales son de utilidad en la prevención y tratamiento de varias de las patologías que afectan a la población costarricense. La presente revisión, documenta los efectos positivos que algunos nutrientes y otros elementos que aporta el frijol tienen en la prevención y el control de algunas de las enfermedades crónicas que constituyen problemas de salud pública en el país. Se abordan de manera particular la fibra, el ácido fólico y el magnesio. Asimismo, se hace referencia a otras sustancias fitoquímicas, presentes en los frijoles, que constituyen elementos protectores de la salud y que hacen de los frijoles un "alimento funcional". Finalmente, se ofrecen recomendaciones prácticas orientadas a mejorar la digestibilidad de este alimento, de manera que se contribuya a mantener e incrementar su consumo a nivel de los hogares costarricenses.

  14. Comportamiento agroproductivo de 10 variedades de frijol común (Phaseolus vulgaris L. en el agroecosistema del municipio de Yaguajay.

    Directory of Open Access Journals (Sweden)

    Wilfredo Valdivia Pérez

    2015-09-01

    Full Text Available Se presentan los resultados del comportamiento agroproductivo de 10 variedades de frijol común (Phaseolus vulgaris L., en las condiciones edafoclimáticas de la CCS “Luis La O” del municipio Yaguajay, provincia de Sancti Spíritus. El estudio se llevó a cabo en el período diciembre 2013- marzo 2014.Con un marco de siembra de 0,50 m x 0,10 m, para 200 000 - 250 000 plantas por hectárea. Utilizamos un diseño Latinizado con tres bloques de 10 parcelas distribuidas al azar y de 5 m2 cada una. El estudio fenológico manifestó que las variedades más precoces resultaron: Velazco Largo y Cul-156. Las de ciclo más largo: CC-25-9 (n y Tomeguín 93. La información reveló diferencias en el comportamiento fenológico de las variedades, al comparar los parámetros del experimento, con los que publica el Instituto de Investigaciones de Granos (IIG. Se apreció que las variedades: Buena Ventura (r y CC-25-9 (r como las de mejor comportamiento en cuanto al rendimiento, con diferencias significativas respecto a las demás variedades. Estas variedades no lograron sus rendimientos potenciales, pero superaron los rendimientos del territorio.

  15. Características morfo-fisiológicas de frijol común (Phaseolus vulgaris L.) relacionadas con la adaptación a sequía

    OpenAIRE

    Polanía, José A; Idupulapati M Rao; Sara Mejía; Beebe, Stephen E.; César Cajiao

    2012-01-01

    El desarrollo de variedades adaptadas es una de las estrategias que contribuye a garantizar la seguridad alimentaria en zonas productoras de frijol con estrés por sequía. En los invernaderos de cristal del Centro Internacional de Agricultura Tropical (CIAT), Palmira, Colombia, se evaluaron las características morfológicas y fisiológicas de raíces de 21 líneas de frijol (Phaseolus vulgaris L.) en condiciones de estrés por sequía e irrigación, utilizando un sistema de tubos plásticos desarrolla...

  16. Mejoramiento de la producción del frijol (Phaseolus vulgaris, L con el uso de alternativas de fertilización

    Directory of Open Access Journals (Sweden)

    Rolando Ramírez Olivera

    2010-01-01

    Full Text Available Argumenta la evaluación de algunas alternativas de nutrición en el frijol común (Phaseolus vulgaris, L., variedad Velasco Largo, que se desarrolló en áreas de la subestación de Granos de la Unidad de Extensión, Investigación y Capacitación Agropecuaria de Holguín (UEICA-H, ubicada en Velasco, municipio de Gibara, provincia de Holguín, en el periodo comprendido entre diciembre de 2007 y marzo de 2008. Se aplicaron seis (6 variantes de fertilización, que comprendió el uso de fertilizantes químicos a dosis menores que las recomendadas, la utilización de abonos orgánicos: humus de lombriz tanto sólido como líquido y el biofertilizante Ecomic. Se tomaron mediciones de altura de las plantas, nodulación y rendimiento y sus componentes, así como, se realizó un análisis económico en base a los rendimientos obtenidos. Los resultados mostraron que la utilización de diferentes alternativas de fertilización provocó un efecto directo sobre el crecimiento de las plantas de frijol, sobre la nodulación natural y el rendimiento y sus componentes, los mismos sugieren la utilización de combinaciones de fertilizantes químicos, orgánicos y biofertilizantes para la obtención de altos rendimientos de forma sostenible en el cultivo del frijol.

  17. Efecto de un promotor del crecimiento activado molecularmente sobre la germinación y la producción de frijol (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Kolima Peña Calzada

    2015-09-01

    Full Text Available El objetivo de la investigación fue evaluar el efecto de un promotor del crecimiento activado molecularmente sobre la germinación y producción del cultivo de frijol (Phaseolus vulgaris L.. Se hicieron dos experimentos, uno in vitro con un diseño completamente aleatorizado y otro en campo con dos variantes y 8 réplicas (diseño de bloques al azar. El tratamiento en ambos ensayos fue inmersión de las semillas en una solución del promotor al 0,02% por 3 horas y otro grupo testigo con inmersión por igual tiempo en agua natural. Los resultados mostraron que la germinación y la altura de las plantas fueron mejores con la inmersión en el producto en ambos experimentos. En el ensayo de campo los vainas por planta no hubo diferencia entre los tratamientos. En los granos por vaina y granos por planta el grupo tratado fue superior al control en 16,70 y 15,22% respectivamente. La masa de 100 granos no mostró diferencia entre ambos tratamientos. El rendimiento agrícola fue superior en los tratados(1,22t.ha-1 vs. 1,02. Con ambos experimentos se determinó que el promotor del crecimiento influyó positivamente en la germinación, desarrollo y producción del cultivo del frijol.

  18. Efectos de la aplicación simultanea de Fitomas E y Biobras 16 en el cultivo del frijol (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Kolima Peña Calzada

    2015-09-01

    Full Text Available El objetivo fue evaluar el efecto de la aplicación simultánea de Fitomas E y Biobras 16 en el cultivo del frijol (Phaseolus vulgaris L.. Se utilizó un diseño de bloques al azar con dos tratamientos y ocho réplicas. Los tratamientos consistieron en la aplicación combinada de Fitomas E (2,0 l.ha-1 y Biobras 16 (0,12 l.ha-1 y una variante control. Las variables evaluadas fueron: altura de las plantas a los 25 y 40 días post siembra, vaina por planta, granos por vainas, granos por plantas, masa de 100 granos y rendimiento agrícola. Se observó en la altura de las plantas diferencias estadísticas significativas entre ambos tratamientos, superando la combinación de Fitomas E y Biobras 16 al tratamiento control, en un 19,43 % y un 17,73 % respectivamente. La variable vainas por planta también mostró diferencias significativas favorables al tratamiento con los bioestimulantes, no así el número de granos por vainas y la masa de 100 granos. El mayor rendimiento agrícola se obtuvo con la variante donde se aplicó Fitomas E y Biobras 16, el cual difirió significativamente del control. Se concluye que el comportamiento agroproductivo del cultivo del frijol (Phaseolus vulgaris L. se favoreció con el tratamiento evaluado.

  19. RESULTADOS PRELIMINARES DEL EFECTO DE LA APLICACIÓN DE BIOBRAS-16 EN EL CULTIVO DEL FRIJOL (Phaseolus vulgaris L.)

    OpenAIRE

    Lissy Rosabal Ayan; Lisbel Martínez González; Yanelis Reyes Guerrero; Miriam Núñez Vázquez

    2013-01-01

    En Cuba anualmente se importan alrededor de 60 000 t de frijoles para poder satisfacer la demanda de la población. El gobierno cubano ha creado p rogramas para la sustitución de importaciones mediante la producción y comercialización de productos naturales que faciliten el incremento de la producción nacional. En este trabajo se evalúa el efecto que el Biobras -16 (BB-16), asperjado a las semillas o foliarmente, ejerce en el crecimiento y rendimiento de plantas de frijol negro variedad Tomegu...

  20. EFECTO DEL ABONO DE SUSTRATO GASTADO DE CHAMPIÑÓN EN EL RENDIMIENTO DE FRIJOL Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Helio Adán García Mendívil

    2014-03-01

    Full Text Available La manipulación de los desechos orgánicos y sus compostas representan una fuente de nutrientes imprescindible para la agricultura sostenible, ya que provee de fertilizantes naturales y reduce la contaminación ambiental, bajando con ello el costo de producción. El objetivo de este estudio fue evaluar el efecto de la aplicación de composta a base de sustrato gastado de champiñón, enriquecida con silicio, al suelo, en cultivo de frijol, a través de mediciones fisiológicas y rendimiento en frijol. El experimento se estableció en campo, sembrándose frijol var. Pinto Saltillo en Huatabampo, Sonora, en un suelo de tipo aluvión ligero. El diseño experimental utilizado fue de bloques completos al azar, compuesto de 4 tratamientos con cuatro repeticiones: 1.- testigo (sin aplicación, 2-2.5, 3 5 y 4-7.5 Mg ha 1 de composta. Las variables evaluadas fueron: clorofila total, tasa fotosintética (µg CO2 S-1 cm-2, número de vainas por planta y rendimiento. Con los datos se realizó un análisis de varianza y las medias se compararon con la prueba de Tukey (P ≤ 0.05. Se observaron respuestas significativas en la mayoría las variables evaluadas, en clorofila total hubo aumentos de hasta 26.2% en el tratamiento dos, para fotosíntesis de 26.1% en el tratamiento cuatro, en el número de vainas 8.7% en el tratamiento tres, siendo este el de mayor rendimiento (P ≤ 0.05 con 2.91 Mg ha-1, seguido del tratamiento dos con 2.89 Mg ha-1, el tratamiento cuatro con 2.85 Mg ha-1, y por último el testigo con 1.4 Mg ha 1. Los resultados sugieren que el empleo de composta es una alternativa de fertilización viable para la producción de frijol.

  1. Relación entre el Vigor Inicial del Frijol Común (Phaseolus vulgaris L.) con la Resistencia a Sequía y a Macrophomina phaseolina (Tassi) Goidanich

    OpenAIRE

    Netzahualcóyotl Mayek Pérez; Jorge Alberto Acosta Gallegos; Cándido López Castañeda

    2002-01-01

    Se estableció un experimento para determinar la relación entre el vigor inicial de la planta de frijol común (Phaseolus vulgaris) y las características responsables de la resistencia a la pudrición carbonosa causada por el hongo Macrophomina phaseolina, bajo condiciones de deficiencia hídrica en invernadero. Las semillas de la línea BAT 477 (resistente, 230 ± 5 mg de peso promedio) y de la variedad Pinto UI-114 (susceptible, 250 ± 5 mg de peso promedio) se sembraron en macetas con suelo inocu...

  2. SEÑALES PRODUCIDAS POR Rhizobium leguminosarum EN LA INTERACCIÓN CON FRIJOL COMÚN (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    María C. Nápoles García

    2016-01-01

    Full Text Available La interacción Rhizobium-leguminosas depende de un complejo intercambio de señales, que se mantiene durante todo el proceso simbiótico y de las que solamente una combinación correcta permitirá una simbiosis eficiente. Estas plantas secretan flavonoides, reconocidos por bacterias compatibles y que inducen sus genes nod. Estos codifican las proteínas que sintetizan y exportan lipoquitooligosacáridos conocidos como factores Nod. Los factores Nod activan los procesos de infección e inician la división celular en la raíz, hasta la formación del nódulo y participan también en la fijación biológica del nitrógeno. Existen evidencias de que el uso de inductores de los genes nod incrementa la nodulación en algunas leguminosas. El objetivo de este trabajo fue estudiar la producción de algunas moléculas señales, inducidas por la isoflavona genisteína en una cepa de R. leguminosarum y evaluar el impacto de esa inducción en el efecto del inóculo sobre plantas de frijol común. La fracción lipídica en los inóculos fue extraída con n-butanol y analizada por cromatografía de capa fina, cromatografía líquida de alta resolución y cromatografía gaseosa acoplada a un espectrómetro de masas. En los inóculos inducidos con genisteína se detectó una cantidad superior de lipooligosacáridos (factores de nodulación y de ácidos grasos de alto peso molecular, con diferencias significativas respecto a los controles sin inducir. En relación con ese enriquecimiento en moléculas señales, los inóculos inducidos con genisteína, mostraron un efecto positivo en las plantas de frijol de la variedad Cubacueto 25-9, con mayor número de nódulos y contenido de clorofila que las plantas no inoculadas (control.

  3. Evaluación de genotipos mejorados de frijol negro Phaseolus vulgaris en Barinas y Monagas, Venezuela

    Directory of Open Access Journals (Sweden)

    Águeda Duran

    2014-12-01

    Full Text Available La investigación se desarrolló en la finca la Alborada, sector Mamon Mamonal, municipio Rojas, en Barinas y finca La Ceiba, sector Aparicio, municipio Piar, estado Monagas en época seca en el período comprendido entre noviembre, 2011 y febrero, 2012, sobre suelos Inceptisoles y Entisoles respectivamente. El objetivo de esta investigación fue evaluar la respuesta agroproductiva de siete genotipos de frijol negro:SA 029, Línea 13, Minita, Balina, Sesentera, Silvinera y la variedad comercial Tacarigua. Se utilizó un diseño experimental de bloques al azar con arreglo bifactorial, los factores fueron la localidad y los genotipos, con siete tratamientos y cuatro repeticiones, distribuidos en 28 parcelas con un área total del experimento de 554,8 m2. El marco de siembra fue de 0,60 x 0,07 m para un total 13200 plantas totales. Se evaluaron la altura de la planta (cm, número de vainas por planta, número de semillas por vaina, peso de 100 semillas (g y el rendimiento (t.ha-1. Los rendimientos en el estado de Barinas fueron superiores para las variedades Balina con 1,10 t/ha y Tacarigua con 1,06 t/ha y en estado de Monagas, la Línea 13 con 1,92 t/ha, seguida de la Tacarigua con 1,68 t. ha-1 y Silvinera con 1,65 t.ha-1. El efecto económico para todas las variedades por localidad y entre ambas fue satisfactorio.

  4. Características morfo-fisiológicas de frijol común (Phaseolus vulgaris L. relacionadas con la adaptación a sequía

    Directory of Open Access Journals (Sweden)

    José A Polanía

    2012-07-01

    Full Text Available El desarrollo de variedades adaptadas es una de las estrategias que contribuye a garantizar la seguridad alimentaria en zonas productoras de frijol con estrés por sequía. En los invernaderos de cristal del Centro Internacional de Agricultura Tropical (CIAT, Palmira, Colombia, se evaluaron las características morfológicas y fisiológicas de raíces de 21 líneas de frijol (Phaseolus vulgaris L. en condiciones de estrés por sequía e irrigación, utilizando un sistema de tubos plásticos desarrollado por el CIAT. Las características evaluadas fueron profundización visual, longitud total de raíces y distribución de raíces a través del perfil del suelo. En campo, utilizando los mismos genotipos de frijol, se evaluaron características relacionadas con la eficiencia de uso de agua mediante la técnica de discriminación de isótopo de carbono y producción de grano. Los resultados en invernadero mostraron que la profundización visual y longitud de raíces entre 60 y 75 cm tienen una correlación significativa (r = 0.56 y 0.50 respectivamente, P < 0.001 con la biomasa aérea de la planta en condiciones de estrés por sequía. En campo, la discriminación del isótopo de carbono en grano se correlacionó (r = 0.32, P < 0.01 con la producción de grano en condiciones igualmente de estrés por sequía. Las líneas de frijol NCB 226, SER 16, SEN 56 y SEA 15 presentaron una mayor longitud de raíces bajo estrés por sequía (28, 24, 29 y 27 m/planta respectivamente en comparación con las demás líneas evaluadas, lo que les permite mayor transpiración y movilización de fotoasimilados, que favorecen una alta producción de granos. Se estima que la profundización visual, la longitud de raíces entre 60 y 75 cm y la discriminación de isótopo de carbono en grano, son características valiosas como criterios de selección en mejoramiento por tolerancia a estrés por sequía en frijol común.

  5. Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L.) en Costa Rica.

    OpenAIRE

    Araya,Carlos M.; Araya, Rodolfo

    2006-01-01

    En esta investigación se evaluaron diversos genotipos de frijol en la búsqueda de fuentes de resistencia a la antracnosis (Colletotrichum lindemuthianum) y a la mancha angular (Phaeoisariopsis griseola). Se evaluó germoplasma del programa nacional de hibridaciones y de tres viveros internacionales distribuidos por el CIAT: VIFURE, VIPADOGEN y CORE COLLECTION. Los ensayos se establecieron en Puriscal (1017 msnm), Alajuela (814 msnm) y Fraijanes (1650 msnm), durante los ciclos agrícolas 97-98 y...

  6. Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L.) en Costa Rica

    OpenAIRE

    Araya,Carlos M.; Rodolfo Araya

    2000-01-01

    En esta investigación se evaluaron diversos genotipos de frijol en la búsqueda de fuentes de resistencia a la antracnosis (Colletotrichum lindemuthianum) y a la mancha angular (Phaeoisariopsis griseola). Se evaluó germoplasma del programa nacional de hibridaciones y de tres viveros internacionales distribuidos por el CIAT: VIFURE, VIPADOGEN y CORE COLLECTION. Los ensayos se establecieron en Puriscal (1017 msnm), Alajuela (814 msnm) y Fraijanes (1650 msnm), durante los ...

  7. Growth dynamics of two bean varieties (Phaseolus vulgaris L.. l. Growth analysis by fixed periods Dinámica de crecimiento de dos variedades de frijol (phaseolus vulgaris L. l. análisis de crecimiento por periodos fijos

    Directory of Open Access Journals (Sweden)

    Maldonado Gustavo

    1993-12-01

    Full Text Available Inthe vegetative phase of growth in bean (Phaseolus vulgaris L., the dry matter gain depends on the physiological behavior and growth habit of the bean plant. The Growth process in relation to growth type were evaluated in the present study. The purpose of the study was to establish growth type and growth index relations in ICA-Cerinza (growth type 1 and
    ICA -Tundama (growth type 11 wich are both bush bean varieties. The plants were sown in 40.5 m2 plots, in rows spaced 0.5 m. and 0.12 m. between plants in a randomized completely block design with 4 replications; samples of 3 plants per plotwere taken 7 days each starting 15 to 78 days after emergence. Total dryweight (TOW Stem dryweight (SOW, leaf dry weight (LOW and total leaf area (TLA were
    determined. Relative growth rate (RGR, net assimilation rate (NAR, leaf area ratio (LAR, leaf weight ratio (LWR, and specific leaf area (SLA, were determined by the classical aproach. Significant differences in TDW, SDW, LDW, and TLA between varieties were detected in the 15 and 78 days
    evaluations. Similar results were obtained for RGR, NAR, LAR, LWR, and SLA. The mean RGR was 0.0455 g. g-1 .day-1 in Cerinza and 0.0437 g.g-1.day -1 in Tundama but these were no statistically different. NAR and RGR had similar trends and were positive and significantly correlated. LAR decreced
    linearly in Cerinza, but it was cuadratic in Tundama with the highest values in the initial and the last evaluations. The LWR show that Tundama variety translocated more dry matter to assimilatory tissue formation. SLA was similar for the two varieties, but it was higher in the indeterminate (type 11, showing that Tundama had moretinny leaves. Growth analysis
    utilizing fixed time periods did not allow to detect differences between varieties. Apparently, these were similar in physiological behavior during the vegetative phase independently of growth type.
    La fase de crecimiento vegetativo de la planta de

  8. Características agronómicas y contenido de Fe y Zn en el grano de frijol tipo Rosa de Castilla (Phaseolus vulgaris L.)

    OpenAIRE

    Yanet Jiménez-Hernández; Jorge Alberto Acosta-Gallegos; Bertha María Sánchez-García; Miguel Ángel Martínez Gamiño

    2012-01-01

    El frijol de tipo Rosa de Castilla (RC) es popular en áreas de Guanajuato, San Luis Potosí y Zacatecas, donde se produce bajo temporal. El objetivo fue comparar características agronómicas de 22 colectas de tipo RC con dos variedades mejoradas de tipo Flor de Mayo (FM) bajo condiciones de temporal. El experimento se estableció en 2008 en dos localidades de Guanajuato, Celaya (1 785 msnm) y Ocampo (2 200 msnm) y una de San Luis Potosí, Villa de Arriaga (2 200 msnm). Se utilizó un diseño de blo...

  9. Efecto de la labranza mínima y la convencional en frijol (phaseolus vulgaris l.) En la región Huetar norte de Costa Rica

    OpenAIRE

    Luis Antonio Rojas; Gerardo Chavez

    2002-01-01

    Se realizó un trabajo en la Región Huetar Norte de Costa Rica en donde se establecieron dos lotes: uno de labranza mínima (1,85 ha) y otro de labranza convencional (2 ha). La siembra en ambos sistemas de labranza se realizó con una sembradora abonadora hidraúlica. La variedad sembrada fue la Brunca. La población de plantas de frijol evaluada durante la etapa vegetativa fue de 9,37 plantas/m lineal en labranza mínima, y de 9,55 plantas/m lineal en labranza convencional. ...

  10. Diagnóstico de la actividad productiva del frijol (Phaseolus vulgaris L.) en la Región Brunca de Costa Rica: Cosecha 1995-1996.

    OpenAIRE

    Hernández, Juan Carlos; Araya, Rodolfo; González, Walter

    2006-01-01

    Se seleccionaron nueve localidades caracterizadas por su tradición en la siembra de frijol para efectuar un monitoreo y diagnosticar la situación de esta actividad productiva. Con base en encuestas y visitas a fincas de agricultores se obtuvo que en la mayoría de las localidades predominó el empleo de variedades criollas, de las que se caracterizaron por su precocidad las de mayor uso comercial. Las variedades mejoradas mostraron como desventaja su mayor ciclo vegetativo lo que influyó en may...

  11. Diversidad agronómica, morfológica y molecular en variedades nativas de frijol (Phaseolus vulgaris L.) cultivadas por agricultores en el estado de Puebla.

    OpenAIRE

    Ramírez Pérez, Ana Rosa

    2011-01-01

    El frijol común representa un cultivo básico en México con una gran variabilidad en sus características morfológicas y moleculares. La descripción del germoplasma de una especie en las regiones donde se cultiva es el primer paso que permite conocer las características principales de las variedades que lo forman, para así propiciar un sistema integral que vincula el estudio experimental con la aplicación de los conocimientos a beneficio del productor. En éste trabajo se hizo la evaluación en c...

  12. Estudio etnobotánico del frijol yepatlaxtle (Phaseolus coccineus L., en el área natural protegida Corredor Biológico Chichinautzin, Morelos, México

    Directory of Open Access Journals (Sweden)

    R. Monroy

    2010-01-01

    alimentación y la comercialización de excedentes y aporta ingresos económicos. En otras localidades de la entidad, aún se cultiva en parcelas pequeñas o en el traspatio. El conocimiento tradicional del manejo y uso del frijol es fundamental como alternativa para su conservación; por tanto, se planteó describir el proceso productivo hasta su comercialización y las formas tradicionales de consumo. El proceso productivo del yepataxtle, desde la siembra hasta la cosecha, se registró durante un ciclo agrícola en una parcela cuyo dueño lo explicó. La comercialización se documentó a través de entrevistas a comerciantes y compradores en el mercado municipal durante los días de selecplaza. Las formas de consumo se obtuvieron a través de entrevistas abiertas a las amas de casa que preparan platillos con el yepataxtle. El sistema milpa, cuando incluye yepataxtle, dura 240 días; cuando es solo, 120. Se explican las técnicas campesinas desde la cosmovisión de los informantes. El yepataxtle se comercializa directamente en el mercado, de febrero a mayo como ejote fresco, y en abril, como frijol seco. La información culinaria tradicional del yepataxtle se organizó en un recetario mediante un uso ceremonial para las fiestas patronales del pueblo.

  13. Determinación de la composición físico-química y de la digestibilidad in vitro de dos variedades de frijol común Phaseolus vulgaris L. estimación del contenido de pectina y celulosa en el residuo indigerible

    OpenAIRE

    2000-01-01

    El propósito de este estudio fue determinar, mediante un estudio de digestibilidad in vitro, la porción indigerible presente en dos variedades de frijol común Phaseolus vulgaris L. consumidas en Costa Rica y cuantificar en dicha porción, dos de los principales polisacáridos no almidonosos causantes de flatulencia: pectina y celulosa. Se determinaron además, las principales características físicas (tamaño y tasa de absorción de agua) y químicas (contenido de proteínas, grasa, cenizas y carbohi...

  14. DETERMINACIÓN DEL PERÍODO CRÍTICO DE COMPETENCIADE LAS ARVENSES CON EL CULTIVO DEL FRIJOL (Phaseolus vulgaris, L.

    Directory of Open Access Journals (Sweden)

    Yaisys Blanco Valdés

    2011-01-01

    Full Text Available El manejo sostenible de arvenses dentro de uncultivo económico de ciclo corto como el frijol (Phaseolusvulgaris, L en el trópico, resulta extremadamente complejo, porla alta diversidad y cantidad de arvenses acompañantes, razonespor las cuales con el florecimiento de los métodos químicos enla década del 70, el uso de herbicidas pre emergentes seimplementó casi universalmente; sin embargo, este método haprovocado el establecimiento de especies altamente agresivas,de difícil manejo, donde hay que acudir a los métodos conven-cionales para lograr bajar sus poblaciones. Por otra parte, se hademostrado, el daño ecológico que provocan los cultivos limpiosde arvenses al eliminarse el refugio de insectos, dado que bajoesas condiciones, se rompe el equilibrio en el agroecosistema,lo que facilita la reproducción de los insectos afines al cultivo,convirtiéndose en organismos plaga. La determinación delperíodo crítico de competencia entre las arvenses y el cultivoeconómico es la única vía eficiente para establecer métodos demanejo de arvenses sólo durante el tiempo que el cultivo lorequiere y de esta forma facilitar la convivencia interespecíficaarvenses-cultivo económico. Para lograr ese objetivo, se llevó acabo una investigación durante dos años donde se evaluómediante un diseño de bloques al azar, con tratamientos a los8, 16, 24, 32, 40, 48 y 54 días después de la germinación con y sinarvenses, y dos testigos, con y sin manejo durante todo el ciclodel frijol. Las arvenses dominantes en el sistema, fueron Cyperusrotundus y Sorghum halepense atribuible a los métodos químicosde manejo precedentes. De acuerdo con los análisis estadísticos realizados el período crítico de competencia de las arvenses conel cultivo del frijol se encontró en el período que transcurredesde 24 a 40 días después de germinado el cultivo, períododurante el cual al cultivo no le pueden faltar las labores de manejode las arvenses

  15. (Helianthus annuus L.-frijol (Phaseolus vulgaris L. en función del nitrógeno y fósforo

    Directory of Open Access Journals (Sweden)

    Edgar J. Morales Rosales

    2007-01-01

    Full Text Available En 2003 se realizó un estudio en Montecillo, Estado de México, para determinar el efecto de la aplicación de nitrógeno (0, 40 y 80 kg ha–1 y fósforo (0, 30 y 60 kg P2O5 ha–1 en la siembra combinada de girasol (cv. Victoria y frijol (cv. Michoacán. Con el suministro de 80 kg N ha–1 (80 – 00 – 00 se incrementó la producción de biomasa y rendimiento de semilla superando al testigo sin fertilizante en 32% y 35%. Cuando se adicionaron 60 kg P2O5 ha–1 (00 – 60 – 00 se obtuvo una producción de materia seca y rendimiento de semilla superior al testigo en 24 y 27%, respectivamente. Aunque la interacción nitrógeno x fósforo no fue significativa, existió una tendencia positiva a medida de que se incrementaron los niveles de nitrógeno y fósforo (80 – 60 – 00, alcanzando los máximos valores en la producción de biomasa (2667.9 g m–2 y rendimiento de semilla (644.6 g m–2, los cuales superaron en 49 y 50% al testigo sin fertilizante.

  16. Efecto de la labranza mínima y la convencional en frijol (phaseolus vulgaris l. En la región Huetar norte de Costa Rica

    Directory of Open Access Journals (Sweden)

    Luis Antonio Rojas

    2002-01-01

    Full Text Available Se realizó un trabajo en la Región Huetar Norte de Costa Rica en donde se establecieron dos lotes: uno de labranza mínima (1,85 ha y otro de labranza convencional (2 ha. La siembra en ambos sistemas de labranza se realizó con una sembradora abonadora hidraúlica. La variedad sembrada fue la Brunca. La población de plantas de frijol evaluada durante la etapa vegetativa fue de 9,37 plantas/m lineal en labranza mínima, y de 9,55 plantas/m lineal en labranza convencional. La densidad de malezas fue mayor en labranza convencional y las malezas de mayor dominancia fueron Ri- chardia scabra, Rottboellia cochinchinensis y Spermacoce sp. El peso seco de malezas fue de 37,6 g de malezas/ cuadrí- cula de 0,25 m2 en labranza mínima, mientras que en el sistema de labranza convencional fue de 46,4 g de malezas/ cuadr ícula. La incidencia y severidad de mustia hilachosa (Tanatheporus cucumeris en el sistema de labranza mínima fue de un 42,8% y de 0,83, respectivamente, mientras que en labranza convencional fue de un 52,2% y 1,22, respectivamente. En el sistema de labranza mínima el rendimiento fue de 930 kg/ha, y en labranza convencional de 676 kg/ha.

  17. Avances en la selección de fuentes de resistencia a las principales enfermedades del frijol común (Phaseolus vulgaris L. en Costa Rica

    Directory of Open Access Journals (Sweden)

    Carlos M. Araya

    2000-01-01

    Full Text Available En esta investigación se evaluaron diversos genotipos de frijol en la búsqueda de fuentes de resistencia a la antracnosis (Colletotrichum lindemuthianum y a la mancha angular (Phaeoisariopsis griseola. Se evaluó germoplasma del programa nacional de hibridaciones y de tres viveros internacionales distribuidos por el CIAT: VIFURE, VIPADOGEN y CORE COLLECTION. Los ensayos se establecieron en Puriscal (1017 msnm, Alajuela (814 msnm y Fraijanes (1650 msnm, durante los ciclos agrícolas 97-98 y 98-99. Los materiales se sembraron en parcelas de un surco de dos a seis metros de largo con una o dos repeticiones y manejo con mínimos insumos. Se evaluó la reacción a la infección natural por antracnosis y mancha angular a las seis y ocho semanas después de la siembra, con base en la escala de severidad de uno a nueve. Se seleccionaron materiales con reacción de uno hasta grado tres para antracnosis y de uno hasta grado cuatro para mancha angular. Del grupo de materiales provenientes del programa de hibridaciones se seleccionaron nueve líneas por su resistencia a ambos patógenos. Posteriormente, sólo cinco mostraron resistencia específica a antracnosis y dos a mancha angular. De los viveros distribuidos por el CIAT, el VIPADOGEN aportó dos líneas que mostraron resistencia combinada a las dos enfermedades, mientras que 25 fueron resistentes exclusivamente a antracnosis, además de mostrar tolerancia a baja fertilidad o a sequía. Del vivero CORE COLLECTION, sólo 26 líneas mostraron resistencia combinada; 82 líneas fueron resistentes a antracnosis y 12 a mancha angular. Las mejores fuentes de resistencia a antracnosis y mancha angular estarán disponibles en un vivero internacional

  18. Gestión de nuevos cultivares de frijol común en las condiciones de la UBPC “Santa Maria 4”

    Directory of Open Access Journals (Sweden)

    Juan Miguel Avila Concepción

    2013-11-01

    Full Text Available Esta investigación se real izó en la UBPC Santa Maria 4 perteneciente a la UEB Antonio Guiteras del municipio Puerto padre, provincia Las Tunas, en el período comprendido entre el 28 de noviembre de 2012 hasta el 16 de febrero de 2013, con el objetivo de evaluar el comportamiento agroproductivo de siete cultivares de frijol común (Phaseolus vulgaris, L. , en un suelo Pardo sialítico mullido carbonatado con vista a incrementar la biodiversidad y elevar el rendimiento agrícola de este cultivo. Los cultivares evaluados fueron (Velazco Largo, Colorado E-120, frijol Rayado, Rojo E-114, Frijol Crema Japones, Rojo E-130, frijol Blanco Español, se utilizó un diseño de bloques al azar con cuatro réplicas, la fitotecnia aplicada fue la tradicional establecida para este cultivo, los aspectos evaluados fueron los siguientes, altura de las plantas, número de hojas, grosor del tallo, altura de la primera vaina, granos por vainas, peso de 100 granos, rendimiento agrícola, incidencia de las principales plagas y valoración económica de la producción. El cultivar que obtuvo mayor rendimiento fue el Velazco Largo y los de menor el Rojo E-114 y el Rayado, los valores obtenidos permiten asegurar que desde el punto de vista económico es factible la realización de estas siembras, pues se pueden obtener incrementos en el ingreso por ventas de $ 6242.90 – 20108.85 pesos por hectárea. En el experimento las condiciones edafoclimáticas fueron favorables en todo el ciclo del cultivo, no se registraron altos niveles de infestación de plagas y enfermedades.

  19. Microflora en semillas de frijol

    Directory of Open Access Journals (Sweden)

    Jos\\u00E9 B. Membre\\u00F1o

    2001-01-01

    Full Text Available Microflora en semillas de frijol (Phaseolus vulgaris L.. Se estudió la microflora bacteriana presente en semillas de frijol y su relación con Xanthomonas campestris pv. phaseoli (Xcp, en 118 genotipos procedentes de VIDAC-98, INTA- Nicaragua, TARS-USDA e Isabela-P.R. Se utilizaron cinco métodos de aislamiento: semilla desinfectada con hipoclorito de sodio, semilla en caldo nutritivo refrigerada por una hora, dispersión de 0,1 ml de suspensión de semillas en medio sólido, siembra líquida de 1 ml de suspensión y semilla en caldo nutritivo, agitado y refrigerado por 24 horas. Se aislaron 104 colonias amarillas de 41 genotipos. Treinta y seis colonias fueron KOH positivo (Gram negativo, 68 negativo (Gram positivo y 34 hidrolizaron almidón. Las colonias de pigmentación amarilla resultaron no patogénicas bajo condiciones de invernadero. Estas se identificaron con el sistema BIOLOG como: Pantoea agglomerans (25, Xanthomonas campestris (2, Enterobacter agglomerans (2, Sphingomonas paucimobilis (2, Pseudomonas fluorescens y Flavimonas oryzihabitans. En adición, los genotipos portaron colonias con pigmentación distinta a la amarilla. En las pruebas de antagonismo se identificaron colonias con actividad de deoxyribonucleasa y de antibiosis a Xcp. De éstas, 15 colonias inhibieron a Xcp significativamente. Se identificaron los hongos Rhizoctonia solani, Penicillium spp., Fusarium spp., Aspergillus flavus, Rhizopus nigricans y Macrophomina phaseolina en un 52,9 % del total de genotipos evaluados

  20. Efecto inhibitorio de los taninos del frijol carioca (Phaseolus vulgaris L. sobre la digestibilidad de la faseolina por dos sistemas multienzimáticos Inhibitory effect of carioca bean tannins on phaseolin digestibility assessed by two multi-enzymatic systems

    Directory of Open Access Journals (Sweden)

    Victoria H. Del Pino

    2003-04-01

    Full Text Available Cantidades variables de dos sistemas multienzimáticos de tripsina-quimotripsina-peptidasa y pepsina-pancreatina, fueron utilizados para evaluar el efecto de los taninos provenientes de frijol Carioca (Phaseolus vulgaris L. sobre la digestibilidad de la faseolina, en las formas nativa y denaturalizada. Esta evaluación hecha por los métodos de caida de pH, de hidrólisis en medio tamponado con posterior medición del grado de hidrólisis con ninhidrina y por la técnica electroforética, demostró el efecto adverso de los taninos condensados sobre la digestibilidad de la faseolina, después de producirse una significativa inhibición en el grado de hidrólisis de esa proteína por los dos sistemas multienzimáticos. Se comprobó la dificultad de hidrólisis de la faseolina cuando esta unida al tanino en el punto de saturación (proporción 5/20 tanino/proteína p/p y mayores, después de detectarse en los perfiles electroforéticos, péptidos de 45.7 y 24KDa, resistentes a la hidrólisis hasta por prolongados períodos de incubación. Esto se debió a la existencia de un complejo de naturaleza refractária no digeríble, incluso en condiciones excesivas de enzima. Los resultados sugieren la unión simultánea del tanino con mas de un ligante a través de sus múltiples grupos hidróxilos libres, formando un complejo proteína-tanino-enzima (PTE* que correspondería a la fracción no digeríble de la faseolina.Variable amounts of the two multienzymatic systems, trypsin-chymotrypsin-peptidase and pepsin-pancreatin, were utilized for evaluating the effect of Carioca's bean tannins on the digestibility of native and denatured phaseolin. Three methods were used for this evaluations: the pH drop method, measurement of degree of hydrolysis by the ninhidrin reaction, and by electrophoretic techniques. The results showed the adverse effect of condensed tannins on phaseolin digestibility, for the two multienzymatic systems. Ours results strongly indicate

  1. Anatomía, fisiología y bioquímica de la raíz de plántulas de frijol tepari  (Phaseolus acutifolius A. Gray var. latifolius) en estrés hídrico

    OpenAIRE

    Orduño Cruz, Andrés

    2012-01-01

    Esta  investigación  se  realizó  para  documentar  los  cambios  morfológicos,  anatómicos, fisiológicos  y  bioquímicos  que  ocurren  en  la  raíz  de  plántulas  de  frijol  tepari  (Phaseolus  acutifolius  A.  Gray  var.  latifolius)  de  los  cultivares  “Brown‐Yellow Red” (RYB),  “Negro”,  “Tohono  Oodham”  y  “Blanco  de  Sinaloa”  que  crecieron  en  sustrato  con  diferentes  potenciales  de  agua  (ΨA  )  de  ‐0.03,  ‐0.07,  ‐0.51  y  ‐2.01  MPa.  De  esta  forma  contribuir  a  r...

  2. Anatomía, fisiología y bioquímica de la raíz de plántulas de frijol tepari  (Phaseolus acutifolius A. Gray var. latifolius) en estrés hídrico

    OpenAIRE

    Orduño Cruz, Andrés

    2012-01-01

    Esta  investigación  se  realizó  para  documentar  los  cambios  morfológicos,  anatómicos, fisiológicos  y  bioquímicos  que  ocurren  en  la  raíz  de  plántulas  de  frijol  tepari  (Phaseolus  acutifolius  A.  Gray  var.  latifolius)  de  los  cultivares  “Brown‐Yellow Red” (RYB),  “Negro”,  “Tohono  Oodham”  y  “Blanco  de  Sinaloa”  que  crecieron  en  sustrato  con  diferentes  potenciales  de  agua  (ΨA  )  de  ‐0.03,  ‐0.07,  ‐0.51  y  ‐2.01  MPa.  De  esta  forma  contribuir  a  r...

  3. Metodología para estimar el coeficiente de heterogeneidad del suelo, el número de repeticiones y el tamaño de parcela en investigaciones con frijol (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Blair Matthew

    2012-03-01

    Full Text Available Con el objeto de estimar el coeficiente de heterogeneidad del suelo (b, sin realizar ensayos de unifor- midad, se propuso una metodología en la cual se usan datos provenientes de ensayos de rendimiento, aislando el efecto de tratamiento de la variable de respuesta. Para definir aspectos metodológicos y mostrar algunos resultados obtenidos en el manejo estadístico de la información, se utilizaron los da- tos de un ensayo de rendimiento de frijol común realizado en el Centro Internacional de Agricultura Tropical (CIAT por el Programa de Mejoramiento de Frijol Andino. El coeficiente de heterogeneidad del suelo se estimó a partir de la ley de varianza de Smith (+958 y la ecuación propuesta por Federer (1963 encontrando valores de 0.59 y 0.66, respectivamente. Teniendo como referencia el valor de b estimado a partir de la metodología de Federer (1963 se recurrió a la metodología de Hatheway (1961 para encontrar la mejor combinación de tamaño de parcela, número de repeticiones y diferencia a de- tectar como porcentaje de la media.

  4. EVALUACIÓN DEL COMPORTAMIENTO AGRO-MORFOLÓGICO A PARTIR DE LA CARACTERIZACIÓN DE LA VARIABILIDAD EN LÍNEAS DE FRIJOL COMÚN (Phaseolus vulgaris L. SEMBRADAS EN ÉPOCA TARDÍA

    Directory of Open Access Journals (Sweden)

    Alexis Lamz Piedra

    2016-01-01

    Full Text Available Entre los principales factores que afectan la producción de frijol se encuentra la mala distribución de variedades para las diversas condiciones ambientales en que se cultiva. El objetivo de este trabajo fue evaluar el comportamiento agro-morfológico, considerando la caracterización de la variabilidad de líneas de frijol común promisorias para siembras tardías. La siembra se realizó el 20 de enero de 2014 con un diseño de bloques al azar y cuatro repeticiones en la finca “El Mulato”, perteneciente a la Cooperativa de Créditos y Servicios Fortalecida (CCSF “Orlando Cuellar”, en el municipio San José de las Lajas, Mayabeque. La evaluación se realizó usando 14 variables agro-morfológicos que incluyeron parámetros fenológicos, morfológicos, rendimiento y sus componentes, y resistencia a la roya (Uromyces appendiculatus. Los resultados del análisis de parámetros estadísticos y componentes principales, permitió detectar la variabilidad genética entre las líneas evaluadas. Además, se detectó que las variables de mayor correlación con el rendimiento fueron el número de vainas por plantas y la masa de 100 granos. De forma general, seis de las líneas evaluadas combinan alto potencial de rendimiento y reacción entre intermedia y altamente resistente ante la roya, destacándose la línea SCR 15, con rendimiento potencial muy superior a los reportados en Cuba hasta la fecha. Esto sugiere evaluar estas líneas en ensayos regionales para una posterior generalización-extensión de los posibles cultivares que contribuyan a enfocar un manejo adecuado de estos genotipos y su empleo como fuentes de resistencia a la roya en los programas de mejoramiento genético del frijol.

  5. Morpho-physiological analysis of adaptive responses of common bean (Phaseolus vulgaris L.) to drought stress /

    OpenAIRE

    Polania Perdomo, José A.,

    2016-01-01

    Bibliografia El frijol común (Phaseolus vulgaris L.) es la leguminosa alimenticia más importante en los trópicos. Es cultivada por pequeños agricultores y por lo general expuesta a condiciones desfavorables con mínimo uso de insumos. La sequía y la baja fertilidad del suelo, especialmente deficiencias de nitrógeno (N) y fósforo (P), son principales limitaciones para el rendimiento del frijol en los sistemas de pequeños productores. El frijol puede derivar parte de su requerimiento de N de ...

  6. Morpho-physiological analysis of adaptive responses of common bean (Phaseolus vulgaris L.) to drought stress

    OpenAIRE

    Polania Perdomo, José A.

    2016-01-01

    El frijol común (Phaseolus vulgaris L.) es la leguminosa alimenticia más importante en los trópicos. Es cultivada por pequeños agricultores y por lo general expuesta a condiciones desfavorables con mínimo uso de insumos. La sequía y la baja fertilidad del suelo, especialmente deficiencias de nitrógeno (N) y fósforo (P), son principales limitaciones para el rendimiento del frijol en los sistemas de pequeños productores. El frijol puede derivar parte de su requerimiento de N de la atmósfera a t...

  7. Características agronómicas y contenido de Fe y Zn en el grano de frijol tipo Rosa de Castilla (Phaseolus vulgaris L.) Agronomic traits and Fe and Zn content in the grain of common Rosa de Castilla type bean (Phaseolus vulgaris L.)

    OpenAIRE

    Yanet Jiménez-Hernández; Jorge Alberto Acosta-Gallegos; Bertha María Sánchez-García; Miguel Ángel Martínez Gamiño

    2012-01-01

    El frijol de tipo Rosa de Castilla (RC) es popular en áreas de Guanajuato, San Luis Potosí y Zacatecas, donde se produce bajo temporal. El objetivo fue comparar características agronómicas de 22 colectas de tipo RC con dos variedades mejoradas de tipo Flor de Mayo (FM) bajo condiciones de temporal. El experimento se estableció en 2008 en dos localidades de Guanajuato, Celaya (1 785 msnm) y Ocampo (2 200 msnm) y una de San Luis Potosí, Villa de Arriaga (2 200 msnm). Se utilizó un diseño de blo...

  8. Consideraciones e importancia social en torno al cultivo del frijol en el centro de México Considerations and social importance of the bean crop in central Mexico

    Directory of Open Access Journals (Sweden)

    Dora Ma. Sangerman-Jarquín

    Full Text Available En México, el grano de frijol por su alto contenido de proteína es básico para la alimentación de su población; ocupa el segundo lugar en importancia nacional después del maíz. La producción de las leguminosas, principalmente el frijol ha disminuido una tasa de 3.2%, con respecto a un crecimiento de población más rápido que la producción. El frijol es una leguminosa que constituye una fuente de proteínas e hidratos de carbono natural; además es abundante en vitamina B como: niacina, ácido fólico y tianina; también proporciona hierro, cobre, zinc, fósforo, potasio, magnesio y calcio, contiene un alto contenido de fibra. Existen múltiples variedades de frijol que se caracterizan por su tamaño, color, forma y tipo de crecimiento. Se considera que en total existen 70 especies; en México estás ascienden a 50, destacan cinco especies que se han domesticado Phaseolus vulgaris L. (frijol común, Phaseolus coccineus L. (frijol ayocote, Phaseolus lunatus L. (frijol comba, Phaseolus dumosus (frijol gordo y Phaseolus acutifolius Gray (frijol tepari. En México se cultivan cerca de 70 variedades, de acuerdo a la norma son: negros, pintos, bayos, amarillos y rosados. El cultivo del frijol presenta características propias y definidas en el marco de la soberanía alimentaria, en virtud de que representa un alimento principal para la población rural y urbana. El programa de mejoramiento genético de frijol del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP del Campo Experimental Valle de México (CEVAMEX, ha contribuido en gran medida en generar la tecnología necesaria para que el país alcance la autosuficiencia alimentaria sostenida en este cultivo. Esta tecnología incluye la liberación de variedades con mayor capacidad de rendimiento y mejor calidad alimentaria y culinaria.In Mexico, common bean is an essential component in the diet of the population due to its high protein content; it ranks second

  9. ALBICAMPO, VARIEDAD DE FRIJOL DE TEMPORAL PARA VALLES ALTOS DE LA MESA CENTRAL

    OpenAIRE

    Dagoberto Garza-García; Ramón Garza-García; Carmen Jacinto-Hernández

    2010-01-01

    Albicampo (Phaseolus vulgaris L.) es una variedad de grano negro opaco pequeño, tipo Jamapa, liberada en 2010 por el programa de frijol del Campo Experimental Valle de México (CEVAMEX) del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Se obtuvo mediante una cruza simple, utilizando selección masal hasta la generación F4. En la F5 se realizó selección individual para resistencia a enfermedades, calidad de grano y características agronómicas. Esta variedad se...

  10. Morpho-physiological characteristics of common bean (phaseolus vulgaris l.) related to drought adaptation

    OpenAIRE

    Polanía, José A; M. Rao, Idupulapati; Mejía, Sara; Beebe, Stephen E.; Cajiao, César

    2013-01-01

    El desarrollo de variedades adaptadas es una de las estrategias que contribuye a garantizar la segu- ridad alimentaria en zonas productoras de frijol con estrés por sequía. En los invernaderos de cristal del Centro Internacional de Agricultura Tropical (CIAT), Palmira, Colombia, se evaluaron las caracte- risticas morfológicas y fisiológicas de raices de 21 lineas de frijol (Phaseolus vulgaris L.) en condiciones de estrés por sequía e irrigación, utilizando un sistema de tubos plásticos desarr...

  11. Características agronómicas y contenido de Fe y Zn en el grano de frijol tipo Rosa de Castilla (Phaseolus vulgaris L. Agronomic traits and Fe and Zn content in the grain of common Rosa de Castilla type bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Yanet Jiménez-Hernández

    2012-04-01

    Full Text Available El frijol de tipo Rosa de Castilla (RC es popular en áreas de Guanajuato, San Luis Potosí y Zacatecas, donde se produce bajo temporal. El objetivo fue comparar características agronómicas de 22 colectas de tipo RC con dos variedades mejoradas de tipo Flor de Mayo (FM bajo condiciones de temporal. El experimento se estableció en 2008 en dos localidades de Guanajuato, Celaya (1 785 msnm y Ocampo (2 200 msnm y una de San Luis Potosí, Villa de Arriaga (2 200 msnm. Se utilizó un diseño de bloques completos al azar. En las localidades de Guanajuato se determinaron características fenologicas y la reacción a enfermedades y en las tres se determino el rendimiento y peso de 100 semillas y a una muestra de semilla de cada material se le determino el contenido de hierro y Zinc. Se observaron diferencias significativas (p 1 300 kg ha-¹, lo obtuvieron las colectas RCG08001, RCA06001 y RCZ06001. El promedio del peso de 100 semillas más alto lo obtuvo la colecta RCSLP 08001 con 40.2 g. Las colectas sobresalientes por contenido de minerales fueron RCG 08011 y RCZ06001 con más de 65 y 40 ppm para Fe y Zn, respectivamente. En comparación con los testigos, las colectas mostraron ciclo largo y susceptibilidad a la roya y al tizón de halo.The 'Rosa de Castilla' type bean is popular in areas of Guanajuato, San Luis Potosí and Zacatecas, where it is produced under rainfed conditions. The aim of this research was to compare the agronomic characteristics of 22 RC accessions with two bred cultivars of the Flor de Mayo type beans grown under rainfed conditions. The trial was established during 2008 under rainfall conditions in two locations in the state of Guanajuato: Celaya (1 785 masl and Ocampo (2 200 masl, as well as one in San Luis Potosí, in Villa de Arriaga (2 200 masl. A complete random block design was used. At the locations in Guanajuato, phenological characteristics and reactions to diseases were established, and in all three locations, seed

  12. Identificación de características morfofisiológicas asociadas a la adaptación a sequía para ser usadas como criterios de selección en mejoramiento de frijol común Phaseolus vulgaris L

    OpenAIRE

    Polanía Perdomo, José Arnulfo

    2011-01-01

    Para contribuir a la seguridad alimentaria de zonas productoras de fríjol bajo estrés por sequía, es pertinente encontrar estrategias que permitan minimizar esta limitante, como el desarrollo de variedades adaptadas. Para aumentar la eficiencia y precisión en el programa de mejoramiento de frijol, es importante establecer buenos criterios de selección, con este objetivo se evaluaron diferentes características morfo-fisiológicas relacionadas con adaptación a sequía para identificar si algunas ...

  13. [Use of Phaseolus vulgaris and Vigna sinensis in a fermented dairy drink].

    Science.gov (United States)

    Granito, Marisela; Trujillo, Lesma; Guerra, Marisa

    2004-06-01

    The objective of this work was to develop a new kind fermented dairy drink, partially substituted with clear varieties of Phaseolus vulgaris (caraota) and Vigna sinensis (frijol). The formulation of fermented dairy drinks included sterile extracts of caraota and frijol, as partial substitutes which replaced milk: 10, 20 and 30%. The mixtures were inoculated with 2% of a mixture of Lactobacillus acidophillus, Streptococcus thermophilus and Bifidobacterium sp. and were incubated at 42 degrees C for 7 hours. Mango and guava jams were used as flavorings at 20%. On the basis of the sensorial evaluation the mixtures 10% frijol-mango, 10% frijol-guava, 30% caraota-mango and 20% caraota-guava were selected. In the selected fermented dairy drinks, the levels of protein, soluble and insoluble fiber, available and resistant starches were increased and the protein digestibility was 81%. The technical feasibility of partial substitution of milk with extracts of Phaseolus vulgaris or Vigna sinensis. For the elaboration of a fermented dairy drink similar to the liquid yogurt kind was demonstrated.

  14. Manejo orgánico de la milpa: rendimiento de maíz, frijol y calabaza en monocultivo y policultivo

    Directory of Open Access Journals (Sweden)

    José Gonzalo Pozas Cárdenas

    2017-04-01

    Full Text Available La milpa es un tradicional policultivo Mesoamericano que incluye maíz (Zea mays L., calabaza (Cucurbita spp. y frijol (Phaseolus vulgaris L.. Se caracteriza por una sinergia entre estos tres cultivos que favorece su rendimiento en conjunto y genera resiliencia ante perturbaciones externas. No obstante, retos actuales ambientales y socio-económicos disminuyen el atractivo de la milpa para los productores por lo que es necesario innovar este sistema de producción sin perder su principal fortaleza, la agrobiodiversidad. En un experimento implementado en 2015 en Piedras Blancas, Estado de México, se compararon todas las posibles combinaciones de policultivos de maíz, calabaza y frijol con los monocultivos de cada una de estas plantas. Todo el manejo fue orgánico y la nutrición se basaba en estiércol compostado de bovino. De todos los tratamientos investigados, la asociación de maíz y frijol demostró la mayor producción total, donde el maíz rindió 7.9 Mg ha‑1 y el frijol 1.9 Mg ha-1. En este policultivo, cada planta de maíz produjo 68.3 g, 1.2 veces superior al rendimiento por planta en monocultivo; y cada planta de frijol 27.5 g, lo que corresponde a su rendimiento en el monocultivo. Todas las demás combinaciones de cultivos (maíz-calabaza, frijol-calabaza y maíz-frijol-calabaza superaron también los respectivos monocultivos en cuanto a su rendimiento total. Con respecto a la calabaza se destacó que las diversas asociaciones incrementaron su rendimiento por planta, pero disminuyeron la producción por planta de maíz y frijol asociados con la calabaza. Se confirmó la sobrecosecha de maíz y frijol asociados comparado con los respectivos monocultivos, lo que subraya el potencial productivo de los sistemas de producción diversificados.

  15. Efecto del antraceno en la estimulación del crecimiento en maíz y frijol

    OpenAIRE

    Beatriz Pérez-Armendáriz; Dolores Castañeda-Antonio; Guadalupe Castellanos; Teresita Jiménez-Salgado; Armando Tapia-Hernández; Daniel Martínez-Carrera

    2011-01-01

    Los efectos de hidrocarburos residuales en suelos agrícolas después de una remediación es un tema de gran interés por sus implicaciones sociales. En Acatzingo Puebla, México se restauró un suelo agrícola contaminado por un derrame de diesel. En este trabajo se evaluó la remoción de antraceno agregado a un suelo de Acatzingo, en concentraciones iniciales inducidas de 200 y 400 mg kg-1, utilizando plantas de importancia agrícola de la región, maíz (Zea mays) y frijol (Phaseolus vulgaris) para l...

  16. Evaluación de alternativas para el manejo integrado de plagas del frijol ejotero en Chapingo, México

    OpenAIRE

    Víctor Manuel Pinto; Priscilia Olivia Cruz Carrada; Samuel Ramírez Alarcón; Juan Fernando Solís Aguilar; Luis Emilio Castillo Márquez

    2004-01-01

    En este trabajo se evaluaron diversas alternativas de manejo para reducir la incidencia de las principales plagas en el cultivo de frijol ejotero (Phaseolus vulgaris L.) en la zona de Chapingo, Estado de México, en comparación con el manejo convencional. Se estableció en un lote experimental de la Universidad Autónoma Chapingo, de mayo a octubre de 1996, bajo dos sistemas de labranza, cuatro fechas de siembra, dos densidades de siembra, y con o sin control químico. También se efectuó un análi...

  17. Estrategias de mercado para el frijol centroamericano

    Directory of Open Access Journals (Sweden)

    Lourdes Mart\\u00EDnez

    2004-01-01

    Full Text Available Estrategias de mercado para el frijol centroamericano. En los últimos años, el sub-sector de frijol en Centro América ha sido testigo de varios cambios dinámicos. Si no se encuentran medidas para incrementar la competitividad regional de este sub-sector, los países centroamericanos experimentarán impactos negativos en las áreas sociales y económicas de considerable magnitud, especialmente ahora que Centro América necesita ajustar su economía a los nuevos requerimientos de mercados mas abiertos, como el Tratado Centroamericano de Libre Comercio con USA (TLC. Vendedores de frijoles a nivel intermediario y de consumo final, así como técnicos de oficinas gubernamentales dedicadas al mercadeo de frijoles en Centro América fueron entrevistados para documentar los cambios experimentados, identificar los obstáculos que frenan la viabilidad del sub-sector e identificar las oportunidades de incremento de mercados en el futuro para los productores y vendedores de frijol. Los resultados muestran que para que continúen siendo competitivos, los países centroamericanos deben incrementar la productividad a través del apoyo a las investigaciones para el mejoramiento de variedades de frijol, especialmente aquellas que los consumidores prefieren por características específicas (e.g. color. También es imprescindible el fortalecimiento de los programas de distribución de variedades mejoradas a los productores; las mejoras en la transmisión de información de mercado a los productores; el fortalecimiento de los contactos entre productores, comercializadores a nivel mayorista y supermercados; y una política más específica de apertura de mercados de exportación, especialmente con los Estados Unidos.

  18. Estrategias de mercado para el frijol centroamericano

    OpenAIRE

    Lourdes Mart\\u00EDnez; Richard Bernsten; Miguel Zamora

    2004-01-01

    Estrategias de mercado para el frijol centroamericano. En los últimos años, el sub-sector de frijol en Centro América ha sido testigo de varios cambios dinámicos. Si no se encuentran medidas para incrementar la competitividad regional de este sub-sector, los países centroamericanos experimentarán impactos negativos en las áreas sociales y económicas de considerable magnitud, especialmente ahora que Centro América necesita ajustar su economía a los nuevos requerimientos ...

  19. Colorado

    Directory of Open Access Journals (Sweden)

    Gerardo Rodríguez Quiroz

    2008-01-01

    Full Text Available La conservación de la biodiversidad cuenta, entre sus principales mecanismos de intervención, con las áreas naturales protegidas. En el alto Golfo de California (AGC se ubica la Reser-va de la Biosfera del Alto Golfo de California y Delta del Río Colorado, en la que subsisten especies de alto valor económico, así como especies en peligro de extinción. Este último factor justificó el establecimiento de la reserva. El estudio analiza la efectividad de la Reserva del Alto Golfo como mecanismo de protección de los recursos naturales, en particular de las que están en riesgo de desaparecer, así como de comprobar si los pescadores han mejorado sus condiciones de vida tras la operación de esa área natural. La exploración se llevó a cabo mediante la aplicación de una encuesta a los pescadores. Se sugiere que es indispensable un gran esfuerzo, de autoridades y grupos organizados, para encontrar soluciones al manejo de la Reserva, a fin fijar un programa que permita la recuperación de las especies en peligro de extinción, elevar la calidad de vida de los pescadores y con ello garantizar un equilibrio entre la conservación y la sustentabilidad de la pesca y de los pescadores en el Alto Golfo de California.

  20. Colorado

    Science.gov (United States)

    2002-01-01

    An early-season snowfall accents the Rocky Mountains through western and central Colorado. This true-color image made from data collected by MODIS on October 26, 2001, highlights the contrast between various irrigated areas and the otherwise dry environment at the foothills of the Rockies. One such example is the city of Denver and its outlying suburbs, which can be seen best in the high-resolution image. In areas that would normally harbor drought-tolerant grasses, shrubs and trees, humans are living, watering their lawns, and farming; those watered, green areas differ substantially from the surrounding hues of brown. Numerous National Parks and Monuments dot the Southwestern U.S. The Great Sand Dunes National Monument is one such park. Running along the western base the Sangre de Cristo Range(just below the image's center), a subsection of the Rockies, the monument possesses some of the highest inland sand dunes in the U.S., with crests reaching over 700 feet.

  1. Controles alternativos para el gorgojo del frijol (Acanthoscelides obtectus Say en granos almacenados a partir de materiales naturales y minerales

    Directory of Open Access Journals (Sweden)

    Carlos Ernesto Lopez-Monzon

    2017-03-01

    Full Text Available Con el fin de controlar el daño del gorgojo del frijol [Acanthoscelides obtectus (Say], se evaluó la efectividad en polvo de raíces deshidratadas de chilca (Senecio salignus DC., frutos deshidratados de pimienta negra (Piper nigrum L., fragmentos de madera de pino (Pinus oocarpa Schiede, maíz pulverizado (Zea mays L., cascabillo de frijol (Phaseolus vulgaris L., cal mezclada con ceniza, y arena de río. Se evaluaron ocho tratamientos, con cuatro repeticiones cada uno, en arreglo totalmente al azar. Los materiales naturales y minerales fueron colectados en el municipio de Malacatancito, Huehuetenango y el ensayo se montó en el laboratorio del Centro Universitario de Nor Occidente (Cunoroc, empleando por réplica un frasco de vidrio con capacidad de 1 L y aplicando a cada uno 100 g de semilla de frijol, 10 parejas de gorgojos adultos y 1 g de polvo de los materiales evaluados, según su tratamiento. El análisis se realizó 60 días después de la infestación, donde se evaluó: el porcentaje de daño del gorgojo del frijol, número de gorgojos vivos, correlación del porcentaje de daño versus número de gorgojos vivos y porcentaje de germinación del frijol. El polvo de pimienta negra y la mezcla de cal con ceniza evidenciaron los mejores resultados con el menor porcentaje de daño (1.64% y 3.55%, respectivamente. Las variables porcentaje de daño y número de gorgojos vivos marcaron una fuerte correlación con valor de r = .892. Se estableció que ninguno de los materiales utilizados influyó en la viabilidad de la semilla, registrándose porcentajes de germinación por arriba del 97% en todos los tratamientos. Se recomienda implementar estos productos en el almacenamiento del frijol, para contrarrestar los daños ocasionados por la plaga, obteniendo así beneficios económicos, sociales y ambientales.

  2. Cultivo in vitro y evaluación de la expresión transitoria del gen uidA en Phaseolus vulgaris L.

    OpenAIRE

    Torrescano De Labra, Luiselva

    2015-01-01

    El frijol común (Phaseolus vulgaris L.) es una de las leguminosas de mayor consumo y distribución en el mundo y existe un gran número de variedades con características favorables, para su cultivo y consumo. En México, es una de las bases de la alimentación; sin embargo, aún enfrenta problemas de susceptibilidad a factores bióticos y abióticos, así como la presencia de componentes antinutricionales..Debido a la importancia del frijol, es conveniente desarrollar y establecer un s...

  3. Albicampo, variedad de frijol de temporal para Valles Altos de la Mesa Central Albicampo, rainfed bean variety for the High Valleys of the Central Mexican Plateau

    OpenAIRE

    Dagoberto Garza-García; Ramón Garza-García; Carmen Jacinto-Hernández

    2010-01-01

    Albicampo (Phaseolus vulgaris L.) es una variedad de grano negro opaco pequeño, tipo Jamapa, liberada en 2010 por el programa de frijol del Campo Experimental Valle de México (CEVAMEX) del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Se obtuvo mediante una cruza simple, utilizando selección masal hasta la generación F4. En la F5 se realizó selección individual para resistencia a enfermedades, calidad de grano y características agronómicas. Esta variedad se...

  4. CARACTERIZACIÓN MOLECULAR DE ACCESIONES CULTIVADAS Y SILVESTRES DE FRIJOL COMÚN DE HONDURAS

    Directory of Open Access Journals (Sweden)

    Marcelino Santiago Guachambala-Cando

    2010-01-01

    Full Text Available Para determinar la diversidad genética de las accesiones de Phaseolus que se conservan en el Banco de Germoplasma, un grupo de 69 accesiones de frijol común (Phaseolus vulgaris L. y de P. coccineus de Honduras, fueron evaluadas con 30 cebadores mediante la técnica de marcadores moleculares RAPD (Polimorfismos de ADN Amplificados al Azar, generándose 341 fragmentos polimórficos de ADN. Las distancias genéticas fueron determinadas según el coeficiente (o índice de similaridad de Dice y el método UPGMA. Estos análisis moleculares identificaron dos grupos a partir de un coeficiente de Dice (CD de 0,48, uno perteneciente a P. vulgaris y el otro a P. coccineus. Las accesiones de P. vulgaris, mostraron una división marcada (CD de 0,64 entre las pertenecientes a los reservorios andino y mesoamericano. Por otro lado, las accesiones mesoamericanas se separaron (CD de 0,76 en cuatro grupos, correspondientes a las accesiones silvestres, las variedades de la raza Jalisco, y las variedades mejoradas y criollas de la raza Mesoamérica. Los resultados confirmaron las distancias genéticas esperadas, incluyendo mayor distancia entre las especies de Phaseolus, seguidas de la separación entre las accesiones del reservorio andino y el mesomericano de P. vulgaris. En el reservorio mesoamericano, las dos accesiones de la raza Jalisco presentaron menor similitud que las de la raza Mesoamérica, y dentro de la raza Mesoamérica, los silvestres separados de los cultivados; adicionalmente, las mejoradas relativamente diferentes a las criollas.

  5. RESPUESTA A BACTERIOSIS COMÚN (Xanthomonas axonopodis p.v phaseoli EN LOS CULTIVARES COMERCIALES DE FRIJOL COMÚN DE CUBA, EN CONDICIONES DE CAMPO. AFECTACIÓN DE LOS RENDIMIENTOS POR EFECTO DE LA INOCULACIÓN

    Directory of Open Access Journals (Sweden)

    Odile Rodríguez Miranda

    2015-01-01

    Full Text Available En el germoplasma de frijol común (Phaseolus vulgaris L., en Cuba, es posible identificar cultivares con niveles de resistencia ante un aislamiento patogénico de Xanthomonas axonopodis pv. phaseoli, a fin de disminuir las pérdidas y aumentar el rendimiento promedio del cultivo en campos infectados con este patógeno. Los objetivos de este trabajo estuvieron dirigidos a evaluar, en genotipos de frijol común respuestas contrastantes frente a la incidencia natural e inoculación del aislamiento patogénico Xap527 (Xanthomonas axonopodis; identificar los cultivares comerciales con mejor comportamiento en follaje y vainas y con menores pérdidas de rendimiento en campos infectados por este patógeno. Los resultados permitieron seleccionar los genotipos de frijol, con buen comportamiento frente al aislamiento de Xap estudiado y se determinó la disminución del rendimiento en los cultivares de frijol por su reacción de susceptibilidad frente a Bacteriosis común, con la inoculación del aislamiento Xap 527, de Cuba

  6. Labranza mínima y efecto alelopático en la producción de frijol común en la Empresa Agropecuaria 19 de Abril de la provincia Mayabeque

    Directory of Open Access Journals (Sweden)

    Idalmis Hernández Escobar

    2013-01-01

    Full Text Available Los métodos de preparación de suelo en particular de labranza mínima, conjuntamente con el aprovechamiento biológico- natural que se suceden en las especies vegetales juega un papel importante en el manejo de los sistemas agrícolas sostenibles. Con el objetivo de evaluar la influencia de la labranza mínima y el efecto alelopático en la producción del frijol común (Phaseolus vilgaris,L cv Tomeguín 93-N se realizo el estudio en áreas productivas de la Empresa Agropecuaria 19 de Abril, Quivicán, provincia Mayabeque, sobre un suelo Ferralítico-típico. Se utilizaron como cultivo precedente al frijol común los residuos de: boniato (Ipomoea batata, L cv CEMSA 78- 354, maíz (Zea mays, L cv. Francisco, girasol (Helianthus annus, L cv. Cuba–Sol y frijol común (Phaseolus vulgaris, L cv. Tomeguín 93- N, los cuales fueron incorporados al suelo a razón de 0,3 kg×m-2. Las variantes objeto de estudio fueron el resultado de los cuatros residuos vegetales empleados incorporados y el testigo. Se utilizó un diseño de bloques al azar constituido por los referidos tratamientos y cuatro réplicas. Las variables respuestas fueron: supervivencia de las plantas (porcentaje de reducción, rendimiento (t×ha-1 y el análisis de los costos y utilidades en el cultivo del frijol común (Phaseolus vulgaris, L. Los resultados reflejan la influencia negativa del método de labranza mínima en la supervivencia y rendimiento del frijol común cuando demuestra el efecto alelopático de los mismos, lo que hacen incosteable la producción del cultivo.

  7. Ecofisiología de seis variedades de frijol bajo las condiciones climáticas de la Región Lagunera Ecophysiology of six bean varieties under the weather conditions of the Lagunera region

    OpenAIRE

    Jorge Armando Chávez-Simental; Vicente de Paul Alvarez-Reyna

    2012-01-01

    En la problemática existente en la Comarca Lagunera sobre la disponibilidad de agua, es importante considerar cultivos alternos que se adapten a las condiciones climáticas de la región. Algunas variedades de frijol (Phaseolus vulagris L.) son susceptibles a sequía lo que limita el óptimo desarrollo del cultivo provocando bajo rendimiento. El objetivo de este trabajo fue determinar la tasa de fotosíntesis neta, tasa de transpiración, conductancia estomatal y rendimiento de seis variedades de f...

  8. Rendimiento del frijol ayocote y maíz del agrosistema asociado en función del número de plantas por mata

    Directory of Open Access Journals (Sweden)

    Néstor Jorge Rojas Victoria

    2017-07-01

    Full Text Available El frijol ayocote (Phaseolus coccineus L. es una leguminosa nativa de México con potencial alimenticio. Para lograr mayor distribución en el espacio, captación de radiación solar y producción de materia seca (MS, el ayocote trepador necesita un soporte. Los soportes convencionales como postes, mallas, rafia, elevan el costo de producción, sin embargo el ayocote puede sembrarse utilizando al maíz como soporte. Para incrementar el rendimiento de grano, se ha recurrido al manejo de la densidad de población y arreglo topológico. El objetivo del estudio conducido en Montecillo, México, en el ciclo primavera-verano de 2014, fue determinar si el número de plantas de frijol ayocote y maíz por mata en asociación, afectan la producción de biomasa, índice de cosecha, rendimiento de grano y sus componentes. Los tratamientos fueron nueve, resultado de la combinación de 1, 2 y 3 plantas de frijol ayocote y de 1, 2 y 3 de maíz por mata. Se registraron las etapas fenológicas, biomasa total (BT, índice de cosecha (IC, rendimiento de grano (RG y sus componentes en ambos cultivos. El diseño experimental fue bloques completos al azar con cuatro repeticiones. Los días a ocurrencia de las fases fenológicas en frijol ayocote y maíz, fueron similares entre tratamientos. En la asociación maíz-frijol los cambios en el número de plantas por mata de ambos cultivos, afectaron la BT, IC, RG y sus componentes tanto en ayocote como en maíz. En el agrosistema ayocote-maíz, la BT, RG y sus componentes, presentaron cambios por el número de plantas de ayocote y de maíz por mata. La combinación para lograr la mayor BT, RG e ingreso neto se logró con tres plantas de ayocote y una de maíz.

  9. Calidad alimentaria y potencial nutracéutico del frijol (Phaseolus vulgaris L.)

    OpenAIRE

    Salvador Horacio Guzmán Maldonado; Jorge Alberto Acosta Gallegos; María de los Ángeles Álvarez Muñoz; Sonia García Delgado; Guadalupe Loarca Piña

    2002-01-01

    Los alimentos derivados de las plantas además de los nutrimentos como son los carbohidratos, proteínas y grasas, entre otros, contienen compuestos conocidos como fitoquímicos que proveen beneficios adicionales a la salud humana, los cuales están presentes en alimentos como cereales, frutas y leguminosas. La evidencia científica continua acumulándose en apoyo al papel de los fitoquímicos en la prevención y tratamiento de enfermedades, como consecuencia del número de componentes que tienen un e...

  10. El proyecto "campana educativa para incrementar el consumo de frijoles"

    Directory of Open Access Journals (Sweden)

    Ligia Rodr\\u00EDguez

    2004-01-01

    Full Text Available El proyecto “Campaña educativa para incrementar el consumo de frijoles”. Se describen las diferentes etapas que se siguen en el desarrollo de un proyecto denominado “Campaña educativa para incrementar el consumo de frijoles”. La Campaña tuvo como objetivo “incrementar el consumo de frijoles en la población urbana de clase media de Costa Rica” y contribuir a contrarrestar la disminución en el consumo de este alimento, que se ha presentado en forma muy marcada en el área urbana. El artículo pretende ofrecer una visión global del mencionado proyecto, de manera que le permita al lector la ubicación dentro del proceso, al mismo tiempo que lo refiere a los artículos específicos que ofrecen el detalle de las diferentes etapas y resultados. La metodología describe las características generales del abordaje que se utiliza y la forma en que se desarrolla cada una de las etapas. Los resultados sumarizan los hallazgos de la evaluación y otros aspectos globales del proyecto. Además, ofrece pautas para un abordaje de la educación nutricional focalizada alrededor de un alimento y sustentada en la realidad social y cultural de la población.

  11. Evaluación de mercados para el frijol centroamericano en comunidades étnicas de EE.UU.

    Directory of Open Access Journals (Sweden)

    Miguel Zamora

    2004-01-01

    Full Text Available Evaluación de mercados para el frijol centroamericano en comunidades étnicas de EE.UU. Este documento presenta información sobre el mercado étnico del frijol centroamericano en EE.UU., describe las oportunidades y dificultades potenciales para estos países, y ofrece información a exportadores potenciales en estos países para aminorar estas dificultades. No existen estudios que describan la demanda étnica potencial por frijol centroamericano en EE.UU. Se determinaron los estados y ciudades con las más altas concentraciones de personas de origen centroamericano en EE.UU. Los costarricenses y panameños no fueron incluidos en este estudio por tener poblaciones relativamente pequeñas en EE.UU. Chicago, Miami, Los Ángeles, San Francisco y Washington D.C fueron visitadas para: a recoger datos sobre la disponibilidad de frijol en tiendas detallistas, b entrevistar consumidores centroamericanos acerca de sus preferencias de frijol, c entrevistar organizaciones centroamericanas, distribuidores e importadores de frijol para entender la dinámica del comercio del frijol. Comerciantes estadounidenses también estaban importando frijol de Honduras. Los frijoles centroamericanos se venden al detalle a precios entre 25-50% mayores que otros frijoles similares. Además, los centroamericanos en EE.UU. prefieren fuertemente los frijoles de su país.

  12. Efecto de labranza, humedad y fertilización en el rendimiento de frijol y la patogenicidad de Macrophomina phaseolina (Tassi) Goid Effects of tillage, moisture and fertilization on the yield of the common bean and the pathogenicity of Macrophomina phaseolina (Tassi) Goid

    OpenAIRE

    Krystal Lira-Méndez; Jaime Roel Salinas-García; Arturo Díaz-Franco; Netzahualcóyotl Mayek-Pérez

    2012-01-01

    El hongo Macrophomina phaseolina (Tassi) Goid. causa la 'pudrición carbonosa' en frijol común (Phaseolus vulgaris L.) y otros cultivos y afecta el crecimiento y el rendimiento de grano. En este trabajo se determinó el efecto de cuatro niveles de labranza (barbecho, destronque-bordeo, subsuelo-bordeo, cero), dos de humedad (temporal, riego) y dos de fertilización (química, 40-20-00 NPK; biológica con la micorriza Glomus intraradices) en el rendimiento de grano de la variedad Negro INIFAP en 20...

  13. CARACTERIZACIÓN DE PROTEÍNAS Y CONTENIDO MINERAL DE DOS VARIEDADES NATIVAS DE FRIJOL DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Juan Carlos Raya-P\\u00E9rez

    2014-01-01

    Full Text Available El objetivo de este trabajo fue caracterizar a nivel bioquímico la semilla de dos variedades mexicanas de frijol, Bayo Berrendo y Patzcuareño. En el año 2011, en Celaya, México, se extrajeron proteínas de reserva de las dos variedades criollas de frijol (Phaseolus vulgaris, con el fin de obtener sus perfiles electroforéticos. El contenido de proteína soluble en el caso de Bayo Berrendo fue de 16,47% y 14,53% para el Patzcuareño. Se detectaron diferencias en el patrón electroforético, sobre todo en la fracción de albúminas, que permitió di- ferenciar las variedades. La harina presentó alta actividad inhibitoria contra tripsina, principalmente en la fracción de faseolinas, 355 unidades en el Bayo Berrendo y 507 unidades en el Patzcuareño. Se determinó la composición elemental de la harina observándose diferencias en cuanto al contenido mineral. El Bayo Berrendo tiene un contenido de 0,15% de Ca, 5,53% de K y 0,60% de Mg. Los valores respectivos para el Patzcuareño fueron: 0,17%, 3,52% y 0,67%. El Bayo posee 18,8 ppm de Fe y el Patzcuareño 3,63 ppm. Se midieron otras variables relacionadas con su utilización con fines alimenticios, como capacidad de absorción de agua, con valores cercanos al 100% y tiempos de cocción menores a 110 min. Fenotípicamente las dos variedades se distinguen fácilmente, tanto en tamaño de semilla como en color. El peso de 100 semillas del Bayo Berrendo fue de 14,6 g y del Patzcuareño 32,6 g.

  14. Efecto del encalado en la respuesta vegetal y fijación simbiótica del nitrógeno en frijol común

    Directory of Open Access Journals (Sweden)

    Aurelio Garc\\u00EDa

    2003-01-01

    Full Text Available En casa de cristales se evaluó mediante el método de valor A, utilizando el isótopo estable 15N, el efecto del encalado de un suelo Rhodic ferralsol sobre la fijación simbiótica de nitrógeno (FSN de los genotipos de frijol común (Phaseolus vulgaris L. BAT 58, BAT 477, DOR 364, CC 25-9 (N. La isolínea NN 125, genotipo de frijol no nodulante, fue utilizado como cultivo de referencia. El efecto del encalado incrementó la fracción de nitrógeno en planta derivado del aire, principalmente en CC 25-9 (N que posee muy poca capacidad de fijación de N atmosf érico cuando no se encala el suelo. Además, se encontró un efecto beneficioso del encalado sobre las reservas de N del suelo, visto que los incrementos de las extracciones totales de N por las plantas provienen del N del aire, e incluso, la cantidad de N en planta derivado del suelo disminuyó para todos los genotipos, principalmente en DOR 364 y CC 25-9(N. Por otra parte, la relación %Nddf/%Ndds, por ciento de N en planta derivado del fertilizante y por ciento de N en planta derivado del suelo respectivamente, varió debido a la aplicación de cal. Por consiguiente, es necesario utilizar dos tratamientos con el cultivo de referencia para evaluar acertadamente la FSN: cultivo de referencia con y sin suelo encalado. Se discute la interpretación del parámetro Eficiencia de Uso del N.

  15. Fuentes fosfóricas de diferente solubilidad para frijol común evaluadas por método isotópico

    Directory of Open Access Journals (Sweden)

    Aurelio Garc\\u00EDa Altunaga

    2005-01-01

    Full Text Available Se condujeronexperimentos en casa de cristales de la Estación ExperimentalLa Renée ubicada en la provincia Habana en el año 2003. Elobjetivo fue evaluar fuentes fosfóricas de diferente solubilidadpara frijol común (Phaseolus vulgarisL. utilizando dos sue-los, Acrisol y Ferralsol ródico, y la técnica de dilución isotópica con 32P. Las fuentes fosfóricas evaluadas fueron roca fosfórica (RF Trinidad de Guedes, RF parcialmente solubilizadaal 50 % con H2SO4(producto denominado comercialmenteFPA50 y superfosfato simple (SS en el Acrisol y superfosfato triple (SFT en el Ferralsol ródico. Los genotipos de frijol empleados en el Acrisol fueron BAT477, DOR 364, DOR390 y Censa, mientras que en el Ferralsol fueron BAT58, BAT477, DOR 364 y CC-25-9(N. Se evaluó producción de mate-ria seca y extracción de Py se determinó la fracción (% y cantidad de Pen planta derivado de los portadores y % de eficiencia de uso del fertilizante. La RF en aplicación directa fuepoco efectiva mientras que los superfosfatos y el FPA50 resultaron efectivos en ambos suelos. Hubo diferencia varietalen la respuesta vegetal y en la utilización del Pde las fuentes,que también dependió de las características del suelo.

  16. EFECTO DE LA ROTACIÓN DE CULTIVOS EN LA INCIDENCIA DEL AMACHAMIENTO (Aphelenchoides besseyi Christie EN FRIJOL

    Directory of Open Access Journals (Sweden)

    N\\u00E9stor Felipe Chaves

    2012-01-01

    Full Text Available El efecto de la rotación de cultivos en la incidencia del amachamiento en frijol se evaluó en la región Brunca de Costa Rica durante 2009 y se complementó con una observación de campo en el 2010. La incidencia en 2009 se cuantificó en las etapas de desarrollo vegetativo (V3 o V4, floración (R6 y llenado de vainas (R8 en planta - ciones comerciales de frijol provenientes de rota - ción con arroz, maíz, chile picante, ayote o frijol. En cada plantación se establecieron al azar 4 microparcelas de 10 m 2 , excepto en la secuencia frijol-frijol, donde fueron solo 2; los datos obte - nidos se transformaron angularmente y con un ANDEVA se separaron las medias entre rotacio - nes. La observación de campo del 2010 se llevó a cabo en una finca con 3 parcelas comerciales de frijol en etapa de floración y en suelos previa - mente sembrados con jengibre, tiquizque y maíz, respectivamente; en cada parcela, se establecie - ron sistemáticamente 10 puntos de muestreo para cuantificar la incidencia de amachamiento y se utilizó la prueba de Chi-cuadrado para deter - minar diferencias entre rotaciones. En el 2009, durante la etapa R8 se presentó una incidencia mínima de amachamiento en la rotación chile picante-frijol (4%, niveles intermedios en las rotaciones ayote-frijol (15% y arroz-frijol (29% y altas incidencias en las rotaciones frijol-frijol (62% y maíz-frijol (64%. En la observación de campo del 2010, durante la etapa de floración se presentaron incidencias de 4% en la rotación jengibre-frijol y de 5% en la rotación tiquizque- frijol, que fueron entre 5 y 6 veces menores que la cuantificada en la rotación maíz-frijol (25%. Con base en los resultados obtenidos, el uso de cultivos como chile picante, jengibre, tiquizque y ayote en el ciclo previo a la siembra de frijol, podría contribuir a bajar la incidencia de amacha - miento y las pérdidas que ocasiona en ese cultivo.

  17. ENCUESTA A PRODUCTORES PARA ORIENTAR EL FITOMEJORAMIENTO DE FRIJOL EN ECUADOR

    Directory of Open Access Journals (Sweden)

    Emmalea Garver Ernest

    2008-01-01

    Full Text Available Se realizó una encuesta a más de cincuenta productores de frijol de los valles de Chota y Mira, ubicados al norte de Ecuador, en el año 2003, con la finalidad de recabar información y plantear objetivos para la implementación de un programa de mejoramiento participativo de frijol en Ecuador. Los productores fueron consultados acerca de las variedades de frijol que están cultivando actualmente, los métodos de producción empleados, los problemas de producción del cultivo y las características que deberían mejorarse en las nuevas variedades. Los principales problemas de producción reportados fueron los daños causados por mosca blanca (Trialeurodes vaporariorum, roya (Uromyces appendiculatus y brúquidos (Acanthoscelides obtectus. Los agricultores han cambiado recientemente las clases comerciales de frijol que tradicionalmente cultivaban en respuesta a la demanda del mercado. En cuanto a las características que deberían poseer las nuevas variedades de frijol, indicaron alto rendimiento y semilla de tamaño grande con color y forma comercial. La costumbre de los agricultores de comprar semilla para cada siembra, y no de producir su propia semilla, fue identifi cada como un obstáculo potencial para el uso y mantenimiento de las nuevas variedades mejoradas de frijol en el área estudiada.

  18. Bayo Azteca, primera variedad mejorada de frijol con resistencia a Apion godmani Wagner Bayo Azteca, first improved bean variety with resistance to Apion godmani Wagner

    Directory of Open Access Journals (Sweden)

    Ramón Garza-García

    2010-12-01

    Full Text Available Bayo Azteca, la primera variedad mejorada de frijol (Phaseolus vulgaris L., con resistencia a una plaga insectil, ha sido obtenida por el programa de frijol del Campo Experimental Valle de México, del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias por cruzas múltiples, esto involucró un largo proceso de mejora genética. Bayo Azteca es la primera variedad mejorada resistente al picudo del ejote (Apion godmani Wagner, un curculiónido que ataca al frijol en las zonas templadas de los valles altos de la Mesa Central de México, causando pérdidas que varían desde 50% hasta 90%. Al igual que otras variedades es también resistente a antracnosis [Colletotrichum lindemuthianum (Sacc & Magn. Briosi & Cav.] y tizón común [Xanthomonas campestris pv phaseoli (Smith Dye]. La nueva variedad fue desarrollada para los Valles Altos de la mesa central desde 1 800 a 2 300 m de altitud. Su hábito de crecimiento es indeterminado tipo III, guías cortas y flor blanca, las vainas son de tamaño mediano a grandes con 5 a 6 granos medianos, tiene alto potencial de rendimiento y plasticidad fenológica. En los Valles Altos, es de precocidad intermedia (102 a 118 días a madurez; Bayo Azteca es además de rápida cocción y con alto contenido de proteína.Bayo Azteca, the first improved bean variety (Phaseolus vulgaris L., with resistance to an insect plague, has been obtained via multiple crossing by bean program of the Valley of Mexico Experimental Station (CEVAMEX, of the National Research Forestry, Agriculture and Livestock Institute (INIFAP, which required a long process of genetic improvement. Bayo Azteca is the first improved variety resistant to bean pod weevil (Apion godmani Wagner, which is a curculionidae that attacks beans in template zones of the Central Highlands of Mexico and causes 50-90% losses. Similar to other varieties, the Bayo Azteca is resistant to anthracnose [Colletotrichum lindemuthianum (Sacc & Magn. Briosi

  19. Diversidad morfológica del frijol ayocote del Carso Huasteco de México Morphologic diversity of ayocote beans from Huasteco Karst of Mexico

    Directory of Open Access Journals (Sweden)

    Patricia Vargas-Vázquez

    2011-09-01

    Full Text Available El frijol ayocote (Phaseolus coccineus L. se cultiva en zonas templadas y subtropicales de México. La colección nacional de la especie incluye 800 accesiones y está resguardada por un banco de germoplasma nacional ubicado en Chapingo, México. En este trabajo se caracterizaron 98 accesiones de frijol ayocote de la subprovincia fisiográfica Carso Huasteco de Puebla, México con base en características fenológicas y del color y dimensión de la semilla. Los colores que predominaron fueron el negro (N1 y variantes de violeta (5R y de beige (10YR y 7.5YR. Además, se registraron las variables climáticas de los sitios de recolección originales de cada accesión. El germoplasma se separó en 2 grupos: uno incluyó 42 accesiones con ciclo biológico tardío (días a madurez fisiológica > 110, semillas grandes (peso de 100 semillas > 40 g y vainas largas (longitud > 8 cm con 1 a 3 semillas, desarrollados por selección empírica de los agricultores locales en sitios con temperaturas mínimas de 2 a 5 °C, y otro incluyó 56 accesiones con ciclo precoz (≤ 110 días, semilla chica (peso ≤ 40 g y vainas cortas (longitud ≤ 8 cm con 2 a 4 semillas, cultivadas en sitios con temperaturas mínimas de 0 a - 2 °C. El cultivo del frijol ayocote del Carso Huasteco se limita a una superficie relativamente pequeña; no obstante, con base en las formas y épocas de cultivo, se observó notable variación y diferenciación fenotípica en el germoplasma local. Futuros trabajos determinarán las medidas de conservación y aprovechamiento de dicho germoplasma.Ayocote beans (Phaseolus coccineus L. are cultivated in temperate and subtropical regions in Mexico. The national collection of the species includes 800 accessions and it is preserved by one national germplasm bank places in Chapingo, Mexico. In this work 98 ayocote bean accessions from sub-province Huasteco Karst of Puebla, Mexico were characterized based on phenological traits as well as seed color

  20. Conocimientos, actitudes y prácticas de madres de escolares con respecto a los frijoles

    Directory of Open Access Journals (Sweden)

    Shirley Rodr\\u00EDguez

    2004-01-01

    Full Text Available Conocimientos, actitudes y prácticas de escolares con respecto a los frijoles. En el presente estudio se identificaron los conocimientos, las actitudes y las prácticas de escolares de tercer y cuarto grados, con respecto a frijoles. Una prueba complementaria sobre actitudes se realizó con los niños de primero, segundo y tercer grados. El estudio se llevó a cabo en el año 1998 en Sabanilla de Montes de Oca, comunidad urbana de Costa Rica. El estudio se realizó como parte de un diagnóstico, previo a una campaña educativa orientada a incrementar el consumo de frijoles. En el mismo se utilizó tanto la metodología cualitativa como la cuantitativa. El estudio determinó que los niños (as tenían conocimientos básicos sobre el valor nutritivo de los frijoles. Además, a la mayoría les gustaban los frijoles. Sin embargo, casi un 10% de los niños (as los rechazaba, y esta actitud fue más marcada conforme aumentaba la edad. También permitió identificar algunas otras barreras que limitaban el consumo de frijoles por parte de los escolares, muchas de las cuales estaban influenciadas por actitudes negativas de los adultos o porque el alimento no estaba disponible en el hogar. Esta información fue importante para orientar la planificación de la campaña educativa arriba mencionada, en la cual los niños (as, por su estrecho contacto con las madres, habían sido seleccionados como grupo interactivo.

  1. PINTO BRAVO: NUEVA VARIEDAD DE FRIJOL PARA EL ALTIPLANO SEMIÁRIDO DE MÉXICO

    OpenAIRE

    Rigoberto Rosales Serna; Jorge Alberto Acosta Gallegos; Francisco Javier Ibarra Pérez; Evenor Idilio Cuéllar Robles

    2011-01-01

    En el Altiplano Semiárido de México el frijol pinto, es una de las principales clases comerciales producidas en condiciones de temporal. Se requieren variedades con grano tolerantes al oscurecimiento de la testa, maduración precoz y semillas de mayor tamaño en relación con Pinto Saltillo, la cual es la variedad más popular en la región. El objetivo fue desarrollar una variedad de frijol superior a Pinto Saltillo en precocidad, tamaño de grano y calidad comercial. El personal del Instituto Nac...

  2. Adaptabilidad de genotipos de frijol resistentes a enfermedades y a suelos ácidos

    OpenAIRE

    Ernesto López Salinas; Óscar Hugo Tosquy Valle; Bernardo Villar Sánchez; Enrique Noé Becerra Leor; Francisco Javier Ugalde Acosta; Javier Cumpián Gutiérrez

    2006-01-01

    La productividad del frijol en la región tropical del sureste de México está limitada principalmente por factores bióticos, entre los que destacan las enfermedades, y factores abióticos como sequía y suelos ácidos. Este trabajo se hizo para identificar genotipos de frijol con adaptación en la región, resistencia a enfermedades y tolerancia a suelos ácidos. Durante el ciclo primavera-verano 1999 y otoñoinvierno 1999-2000, se establecieron seis experimentos conformados por 16 genotipos entre lo...

  3. Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2 infecting common bean Phaseolus vulgaris genotypes show differential infection patterns between gene pools

    Science.gov (United States)

    We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV-1) and Phaseolus vulgaris endornavirus 2 (PvEV-2), in breeding-lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) as well as other Phaseolus species collected from two...

  4. COMPARACIÓN DE LOS COMPONENTES DEL RENDIMIENTO EN VARIEDADES DE FRIJOL EN CONDICIONES DE ACIDEZ Y HUMEDAD RESIDUAL DEL SUELO EN EL SUR DE VERACRUZ

    Directory of Open Access Journals (Sweden)

    Aurelio Morales Rivera

    2015-12-01

    Full Text Available El frijol negro (Phaseolus vulgaris L. se cultiva en suelos ácidos (pH<5 con humedad residual en el sur del estado de Veracruz, México. El cultivo se realiza en unas 6600 ha al final de la temporada de lluvias a mediados de septiembre o principios de octubre. La humedad residual, aunado a la acidez del suelo frecuentemente afectan el periodo de formación de la semilla, reduciendo el rendimiento. El presente trabajo tuvo como objetivo caracterizar variedades de frijol de tipo III en cuanto a su rendimiento de semilla y sus componentes, cultivadas bajo restricciones de humedad residual y acidez del suelo, y altas temperaturas en el ciclo otoño-invierno 2012-2013, en el Municipio de Juan Rodríguez Clara, Veracruz. Se emplearon seis variedades de testa de color negro, seis del tipo ‘Flor de Mayo’ y una del tipo ‘Flor de Junio’. El diseño experimental fue de bloques completos al azar con cuatro repeticiones. La unidad experimental se constituyó de cinco surcos de 5 m de longitud y 0.8 m de separación. Se fertilizó con una dosis de 40-40-00. Las variedades “Negro Veracruz”, “San Andrés”, “Negro Cotaxtla” y “Flor de Junio Marcela”, exhibieron mayor rendimiento de semilla, vainas por m2 y peso de 200 semillas, altura de planta y menor biomasa aérea final que las demás. El mayor rango de variabilidad genética entre las variedades se observó para la biomasa aérea final y altura de planta. La respuesta en rendimiento y sus componentes sugiere que algunas variedades de testa negra han desarrollado mecanismos de tolerancia a la acidez, restricción de humedad residual del suelo y altas temperaturas durante la floración y formación de la semilla, que les permiten producir altos rendimientos a pesar de estar sometidas a estrés ambiental. La variedad de Valles Altos (“Flor de Junio Marcela”, también mostró que podría tener buena adaptación a estas condiciones.

  5. Interferencia de malezas en el cultivo de frijol en dos sistemas de labranzas Weeds influence in bean crop in two tillage systems

    Directory of Open Access Journals (Sweden)

    M.C. Parreira

    2013-06-01

    Full Text Available Con este trabajo, se busco determinar el período anterior a la interferencia de las malezas que conviven con el cultivo de frijol, en ausencia o presencia de residuos vegetales de Crotalaria juncea. Los tratamientos estaban constituidos de siete períodos de convivencia del cultivo con las malezas: 0-10, 0-20, 0-30, 0-40, 0-50, 0-60 y 0-70 días después de la emergencia y también de un testigo sin presencia de las malezas en ausencia o presencia de crotalaria. El diseño experimental utilizado fue de bloques completos seleccionados al azar, con cuatro repeticiones por tratamiento. Hubo reducciones de 40,6% y 55,1% en la productividad de granos de frijol en convivencia con malezas durante todo el ciclo del cultivo, en ausencia y presencia de los residuos vegetales, respectivamente. Se concluyó que la productividad de granos fue afectada negativamente a partir de 29 y 38 días después de la emergencia, sin y con residuos, respectivamente, y eso constituyó los períodos anteriores a la interferencia.The research aim to determine the period prior to weed interference on common beans (Phaseolus vulgaris. The treatments had been made out of seven periods of coexisting by the culture with the weed 0-10, 0-20, 0-30, 0-40, 0-50, 0-60, and 0 70 days after crop emergence in absence and presence crop residue. It had reduction of 40.56% and 55.12%, the productivity of grains in the culture of the common bean when the coexistence with the weed was during all the cycle of the culture, in absence and presence crop residue, respectively. It was concluded that grain yield was negatively affected from the 29th and 38th days after emergence, in the absence and presence of crop residue, respectively.

  6. Producción de genotipos de frijol negro en condiciones de humedad residual y sequía terminal

    National Research Council Canada - National Science Library

    Oscar Hugo Tosquy Valle; Ernesto López Salinas; Rigoberto Zetina Lezama; Bernardo Villar Sánchez; José Raúl Rodríguez Rodríguez

    2017-01-01

    ... de sequía durante la etapa reproductiva, lo que causa bajos rendimientos. El objetivo de esta investigación fue determinar el rendimiento de genotipos de frijol, evaluados bajo condiciones de humedad residual...

  7. Estrategias para seleccionar frijol común con mayor resistencia a mustia hilachosa

    Directory of Open Access Journals (Sweden)

    James S. Beaver

    2002-01-01

    Full Text Available Para aumentar el rendimiento y calidad de grano de frijol producido en las regiones calientes y húmedas de Centroamérica y el Caribe, es necesario contar con mayores niveles de resistencia a la mustia hilachosa. El frijol común solamente tiene niveles moderados de resistencia a esta enfermedad y los patrones de virulencia del patógeno (Thanatephorus cucumeris (Frank Donk, varían entre regiones geográficas. Las evaluaciones en el campo en busca de resistencia a la mustia hilachosa están limitadas a las épocas favorables al desarrollo de la enfermedad, y no permiten diferenciar entre plantas que evitan la enfermedad debido a características de arquitecturas y aquellas que poseen resistencia fisiológica. Se han desarrollado técnicas de laboratorio e invernadero que facilitan la evaluación de líneas de frijol con respecto a su resistencia fisiológica. Se propone un esquema de selección recurrente para seleccionar frijoles con mayores niveles de resistencia y el uso de cruces interespecí- ficos podría aumentar la base de genes resistentes a la mustia hilachosa. Se han identificado líneas de P. coccineus con buenos niveles de resistencia a la mustia hilachosa y las plantas de frijol transgénicas con tolerancia a herbicidas serían útiles para labranza mínima. Una cobertura de vegetación muerta, podría reducir también la difusión de la enfermedad.

  8. Phaseolus immature embryo rescue technology.

    Science.gov (United States)

    Geerts, Pascal; Toussaint, André; Mergeai, Guy; Baudoin, Jean-Pierre

    2011-01-01

    Predominant among the production constraints of the common bean Phaseolus vulgaris are infestation of Ascochyta blight, Bean Golden Mosaic virus (BGMV), and Bean Fly. Interbreeding with Phaseolus -coccineus L. and/or Phaseolus polyanthus Greenm has been shown to provide P. vulgaris with greater resistance to these diseases. For interspecific crosses to be successful, it is important to use P. coccineus and P. polyanthus as female parents; this prevents rapid reversal to the recurrent parent P. vulgaris. Although incompatibility barriers are post-zygotic, early hybrid embryo abortion limits the success of F1 crosses. While rescue techniques for globular and early heart-shaped embryos have improved in recent years, -success in hybridization remains very low. In this study, we describe six steps that allowed us to rescue 2-day-old P. vulgaris embryos using a pod culture technique. Our methods consisted of (i) pod culture, (ii) extraction and culture of immature embryos, (iii) dehydration of embryos, (iv) germination of embryos, (v) rooting of developed shoots, and (vi) hardening of plantlets.

  9. Albicampo, variedad de frijol de temporal para Valles Altos de la Mesa Central Albicampo, rainfed bean variety for the High Valleys of the Central Mexican Plateau

    Directory of Open Access Journals (Sweden)

    Dagoberto Garza-García

    2010-12-01

    Full Text Available Albicampo (Phaseolus vulgaris L. es una variedad de grano negro opaco pequeño, tipo Jamapa, liberada en 2010 por el programa de frijol del Campo Experimental Valle de México (CEVAMEX del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP. Se obtuvo mediante una cruza simple, utilizando selección masal hasta la generación F4. En la F5 se realizó selección individual para resistencia a enfermedades, calidad de grano y características agronómicas. Esta variedad se distingue de otras del mismo tipo comercial, por su alto rendimiento, excelente calidad culinaria y alto contenido de proteína, así plasticidad fenológica que le permite adaptarse a zonas, tanto de crítico como de buen temporal de lluvia. Es resistente a antracnosis (Colletotrichum lindemuthianum Sacc & Magn. Briosi & Cav., tizón común (Xanthomonas campestris pv phaseoli (Smith Dye y pudrición de raíz (Pythium y Rhizoctonia, y tolerante a roya (Uromyces appendiculatus (Pers.:Pers. Unger. Es de ciclo intermedio (105 a 115 días a madurez, su hábito de crecimiento es tipo III (indeterminado, con guía corta y flores moradas. Albicampo expresa su máximo potencial en áreas desde 2000 hasta 2400 msnm, suelo profundo y un mínimo de 350 mm de precipitación pluvial.Albicampo (Phaseolus vulgaris L., a small opaque dry bean seed variety from the Jamapa class, was released in 2010 by the bean program of the INIFAP's Campo Experimental Valle de Mexico. It was obtained through a simple cross, using bulk selection to the F4 followed by individual selection in F5 for disease, quality and agronomic traits. It distinguishes from other varieties of the same commercial class because of its high yield (>2 t ha-1, excellent cooking quality and high protein content, as well as phenological plasticity which enables it to adapt itself from critical to good rain season. It is resistant to anthracnose (Colletotrichum lindemuthianum Sacc & Magn. Briosi & Cav

  10. OLEORRESINA DE JÍCAMA Y CALIDAD DE SEMILLA DE FRIJOL INFESTADA CON Acanthoscelides obtectus Say

    OpenAIRE

    Jos\\u00E9 Antonio Rangel-Lucio; Jos\\u00E9 Mayolo Ju\\u00E1rez-Goiz; Edmundo Garc\\u00EDa-Moya; Mar\\u00EDa Dolores Fern\\u00E1ndez-Andr\\u00E9s; Ces\\u00E1reo Rodr\\u00EDguez-Hern\\u00E1ndez; Est\\u00E9fana Alvarado-B\\u00E1rcenas

    2011-01-01

    El objetivo de este trabajo fue determinar el efecto de la oleorresina de jícama en la calidad de la semilla de frijol. En laboratorio se obtuvo el extracto de oleorresina media nte HPLC y se detectó la presencia de rotenona (15 mg/l). Se ensayaron tres concentraciones (Ci, g/ml) del extracto: C1 (5x10-7, 5x10-6, ¿5x10-2); C2 (1x10-2, 2x10-2,¿6x10-2); C3 (5x10-1, 6x10-1,¿9x10-1) y testigo por concentración, en frascos de 300 ml con 50 g de semilla de frijol (varie dades Flor de Ma yo, Flor de...

  11. OLEORRESINA DE JÍCAMA Y CALIDAD DE SEMILLA DE FRIJOL INFESTADA CON Acanthoscelides obtectus Say

    Directory of Open Access Journals (Sweden)

    Jos\\u00E9 Antonio Rangel-Lucio

    2011-01-01

    Full Text Available El objetivo de este trabajo fue determinar el efecto de la oleorresina de jícama en la calidad de la semilla de frijol. En laboratorio se obtuvo el extracto de oleorresina media nte HPLC y se detectó la presencia de rotenona (15 mg/l. Se ensayaron tres concentraciones (Ci, g/ml del extracto: C1 (5x10-7, 5x10-6, ¿5x10-2; C2 (1x10-2, 2x10-2,¿6x10-2; C3 (5x10-1, 6x10-1,¿9x10-1 y testigo por concentración, en frascos de 300 ml con 50 g de semilla de frijol (varie dades Flor de Ma yo, Flor de Junio, Ma - yocoba y veinte gorgojos adultos. El ensayo sólo comprendió semillas de frijol tratadas con C2 por 48 horas para evaluar vigor y germinación estándar a los tres y sie te días después de la siembra, bajo normas de IS TA. El aumento de C2 fue gradual en el número de plántulas normales y germinación estándar en el primer recuento, en particular con las diluciones 3 x 10-2 a 6 x 10-2; en el segundo recuento se confirmó la germinación estándar total con estas mismas diluciones. El frijol Ma yocoba en presencia del extracto de semilla de jícama, mantuvo el vigor y valores porcentuales aceptables de germinación estándar de la semilla.

  12. Influence of double cropping on growth and yield of dry beans with colored plastic mulches Influencia del doble cultivo en el crecimiento y rendimiento de frijol con cubierta plástica de colores

    Directory of Open Access Journals (Sweden)

    Luis Ibarra-Jiménez

    2012-12-01

    Full Text Available There are numerous studies of the use of plastic mulches in vegetable production, but there is little documentation of their use with dry beans (Phaseolus vulgaris L. in single and double cropping. The objective of this study was to grow dry beans over two consecutive growing seasons using the same plastic mulch of different colors and examine the influence of soil temperature on growth and yield. The experiment was conducted in Saltillo, Coahuila, Mexico, in the spring and summer of 2008. The treatments included four colored plastic mulches: white-on-black, black, silver-on-black, aluminum-on-black, and bare soil as a control. The experimental design was a randomized complete block with four replicates. The percentage of photosynthetically active radiation (PAR reflected from the plastic tended to be highest with the white-on-black mulch and lowest with the black mulch. Mean soil temperature under the plastic mulch decreased with the increasing percentage of reflected PAR. Mean soil temperature exhibited a relationship of 98% and 99% to yield in the first and second growing season, respectively. Photosynthetically active radiation had a relationship of 98% and 86% to yield in the first and second growing season, respectively. The effect of the colored plastic mulch on yield was significant (p Existen numerosos estudios sobre el uso de cubierta plástica en la producción de hortalizas, sin embargo está muy poco documentado el cultivo simple o doble de frijol (Phaseolus vulgaris L.. El objetivo de este estudio fue cultivar frijol en dos estaciones de crecimiento consecutivas usando la misma cubierta plástica de diferentes colores y examinar la influencia de la temperatura del suelo en el crecimiento y rendimiento de frijol. El estudio fue conducido en Saltillo, Coahuila, México, en la primavera y verano de 2008. Se usaron cuatro colores de acolchado plástico: blanco-sobre-negro, negro, plata-sobre-negro, aluminio-sobre-negro, y control de

  13. Evaluación de la Disponibilidad de Minerales en Harinas de Frijol y en Mezclas de Maíz/Frijol Extrudidas Evaluation of Mineral Availability in Cowpea Flour and in Maize/Cowpea Extruded Mixtures

    OpenAIRE

    Drago, Silvina R.; González, Rolando J.; Luis Chel-Guerrero; Mirta E Valencia

    2007-01-01

    En el presente trabajo, se analizaron los efectos de la extrusión sobre la disponibilidad de minerales, utilizando mezclas de maíz y frijol (con y sin tratamiento previo de inactivación). Al formular alimentos destinados a programas alimentarios se debe considerar el contenido y la calidad proteica y la biodisponibilidad de nutrientes. Una alternativa explorada en este trabajo es utilizar harinas de frijol, asegurando la calidad organoléptica del producto mediante tratamientos de inactivación...

  14. Respuesta de frijol al Endospor 33® a dosis 50% de fertilizante nitrogenado/fosfatado en agricultura protegida

    Directory of Open Access Journals (Sweden)

    Juan Manuel Sanchez-Yañez

    2014-01-01

    Full Text Available El frijol requiere fertilización nitrogenada y fosfatada (FNP, cuya indiscriminada aplicación causa pérdida de productividad del suelo, una alternativa de solución para este problema es reducir y optimizar la dosis de FNP con un inoculante mixto con bacterias promotoras de crecimiento vegetal (BPCV y hongos micorricicos vesículo arbusculares (HMA. El objetivo de esta investigación fue analizar la respuesta del frijol al inoculante mixto Endospor 33® a dosis reducida al 50% del FNP. En un suelo ferralítico rojo pobre de Nitrógeno (N y materia orgánica. Con un diseño experimental de bloques al azar con 5 tratamientos y 6 repeticiones. Los resultados indican que el porcentaje de germinación del frijol con Endospor 33® a la dosis 20 mg/semilla fue de 100%. A plántula con 10 mg/planta, tuvo 6,13g de peso fresco total (PFT y de 0,66g de peso seco total (PST comparado con el frijol control relativo (CR con 0,28g de PST con el 100% del FNP. A floración con 30 mg/planta Endospore 33® tuvo 1,5g de PST comparado con 1,07g de PST del frijol CR. A madurez fisiológica con 30mg/planta registro 34,83g/100 semillas, en contraste a su homologo CR con 20,39g/100 semillas. Lo anterior indica que este inoculante mixto es una opción en la producción del frijol a dosis reducida del FNP, sin afectar negativamente su crecimiento y/o rendimiento.

  15. Validación de genotipos de frijol negro en la zona central del Estado de Veracruz, México

    OpenAIRE

    2005-01-01

    Validación de genotipos de frijol negro en la zona central del estado de Veracruz, México. El objetivo del presentetrabajo, fue validar genotipos de frijol con el paquete tecnológico del INIFAP, en parcelas de productores. Durante los ciclosde humedad residual de 1998 a 2001 se establecieron nueveparcelas de validación. Los genotipos utilizados fueron: las lí-neas II-307 y DOR-500 y las variedades Negro Huasteco 81,Negro Cotaxtla 91 y Negro INIFAPy como testigo del productor la variedad Jamap...

  16. UTILIZACIÓN DE EXTRACTOS DE PLANTAS PARA EL CONTROL DE GORGOJO PARDO ACANTHOSCELIDES OBTECTUS (SAY) EN FRIJOL ALMACENADO

    OpenAIRE

    Eusebio Nava-Pérez; Patricia Gastélum-Hurtado; Jesús Ricardo Camacho-Báez; Benigno Valdez-Torres; Carlos Ramón Bernal-Ruiz; René Herrera-Flores

    2010-01-01

    El frijol es uno de los principales cultivos en el norte de Sinaloa. Las mayores pérdidas ocurren durante su almacenamiento y éstas son ocasionadas por enfermedades y plagas, como el gorgojo pardo del frijol (Acanthoscelides obtectus Say). Para controlar este tipo de plagas se aplican malatión, fosfuro de aluminio y bromuro de metilo. Debido a la alta toxicidad de estos productos, se utilizaron, extractos de Bacharis glutinosa, Eucaliptus globulos y Melia azedarach, para el control del gorgoj...

  17. Digestibilidad de garbanzo, maíz alta calidad proteica y frijol quebrado en tilapia Oreochromis niloticus

    OpenAIRE

    Montoya Mejía, Magnolia

    2014-01-01

    El garbanzo, maíz de alta calidad proteica y frijol quebrado, son cereales y leguminosas de gran producción en la región de Sinaloa, al momento de la cosecha algunos granos se quiebran y estos son considerados como granos de segunda, estos pueden ser utilizados como ingredientes en la formulación de alimentos balanceados para la acuicultura. El objetivo del presente trabajo fue determinar la digestibilidad de garbanzo, maíz de alta calidad proteica y frijol quebrado in vivo en ...

  18. Transcriptome Sequencing of Lima Bean (Phaseolus lunatus to Identify Putative Positive Selection in Phaseolus and Legumes

    Directory of Open Access Journals (Sweden)

    Fengqi Li

    2015-07-01

    Full Text Available The identification of genes under positive selection is a central goal of evolutionary biology. Many legume species, including Phaseolus vulgaris (common bean and Phaseolus lunatus (lima bean, have important ecological and economic value. In this study, we sequenced and assembled the transcriptome of one Phaseolus species, lima bean. A comparison with the genomes of six other legume species, including the common bean, Medicago, lotus, soybean, chickpea, and pigeonpea, revealed 15 and 4 orthologous groups with signatures of positive selection among the two Phaseolus species and among the seven legume species, respectively. Characterization of these positively selected genes using Non redundant (nr annotation, gene ontology (GO classification, GO term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG pathway analyses revealed that these genes are mostly involved in thylakoids, photosynthesis and metabolism. This study identified genes that may be related to the divergence of the Phaseolus and legume species. These detected genes are particularly good candidates for subsequent functional studies.

  19. Defoliación en maíz y su efecto sobre el rendimiento de frijol-maíz en asociación

    OpenAIRE

    Rafael Delgado Martínez; José Alberto Salvador Escalante Estrada; Ramón Díaz Ruíz; Antonio Trinidad Santos; Edgar Jesús Morales Rosales; Eliseo Sosa Montes

    2014-01-01

    El rendimiento tanto de maíz y frijol en monocultivo está determinado por el genotipo y el ambiente. Sin embargo, la siembra asociada de estos cultivos puede incrementar la producción por unidad de área. No obstante, el efecto de competencia interespecifica donde el frijol es más afectado y que se asume al sombreado del maíz sobre el frijol ha sido poco atendido. El objetivo del estudio fue determinar el efecto de la defoliación del maíz sobre la biomasa total (BT), índice de cosecha ...

  20. DISTRIBUCIÓN ESPACIAL DEL AMACHAMIENTO DEL FRIJOL (Aphelenchoides besseyi CHRISTIE EN CAMPO

    Directory of Open Access Journals (Sweden)

    N\\u00E9stor Felipe Chaves-Barrantes

    2014-01-01

    Full Text Available El objetivo del presente trabajo fue determinar la distribución espacial del amachamiento en campo. La investigación se realizó entre mayo y julio del 2009 en las comunidades de Veracruz de Pérez Zeledón, San José y Concepción de Buenos Aires, Puntarenas, Costa Rica. Para ello se evaluó la incidencia de la enfermedad por punto de siembra en dos parcelas de frijol provenientes de distintas rotaciones (arroz/frijol y maíz/fri - jol, con lo cual, posteriormente se determinó la distribución espacial de la enfermedad en campo por medio de un análisis de corridas y los índices de varianza/media (V/M, Lloyd y Morisita. En el análisis de corridas se obtuvo un valor pro - medio de Z de -1,06 para la parcela sembrada previamente con arroz, y de -0,98 para la parcela sembrada previamente con maíz, lo que indica una tendencia a la agregación. Los índices de V/M, Lloyd y Morisita fueron mayores que 1 en ambas parcelas, por lo que se determinó que el amacha - miento presentó un patrón espacial agregado (en parches en condiciones de campo. El patrón espacial del amachamiento en campo tiende a enmascararse debido a la rotación con cultivos hospederos de Aphelenchoides besseyi , – su agente causal –, en especial la rotación maíz/frijol donde el produc - tor redistribuye el inóculo de la enfermedad cuando esparce las cañas de maíz sobre el terreno de cultivo

  1. Producción artesanal de semilla de frijol en Veracruz, México

    Directory of Open Access Journals (Sweden)

    Ernesto L\\u00F3pez

    2001-01-01

    Full Text Available Para solucionar la escasez se semilla mejorada de frijol en los municipios de Veracruz y Jamapa se estableció el proyecto de Producción Artesanal de Semillas de Frijol (PASF, desde el ciclo 1998/99, con un modelo de coparticipación de las Jefaturas de Fomento Agropecuario de los Ayuntamientos, productores e investigadores del programa de frijol. En el primer ciclo se establecieron parcelas demostrativas y de validación con variedades y líneas, respectivamente y se capacitó a los agricultores aprendiendo - haciendo. En el siguiente ciclo se multiplicaron las variedades con productores asesorados por los investigadores y se hizo la entrega de semilla PASF. Con base en cuatro toneladas de semilla y el pago por parte de las municipalidades de Veracruz y Jamapa del 50% del costo de la semilla, 118 productores sembraron 100 hectáreas en el ciclo otoño-invierno 99-2000; además se distribuyeron 800kg a agricultores frijoleros. En las parcelas comerciales los rendimientos fueron variables debido a la lluvia irregular; en el municipio de Veracruz, las variedades PASF promediaron 550 kg y en Jamapa fueron de 650 kg/ha. Estos rendimientos fueron superiores a los obtenidos con semilla de programas emergentes y a los criollos regionales (300 kg/ha. La estrategia propuesta es una alternativa viable para satisfacer la demanda de semilla municipal e iniciar un proceso de adopción entre los agricultores. Este modelo se está promoviendo

  2. Efectos de una campaña educativa sobre frijoles en escolares

    Directory of Open Access Journals (Sweden)

    Ligia Rodr\\u00EDguez

    2004-01-01

    Full Text Available Efectos de una Campaña Educativa sobre frijioles en escolares. Una “Campaña Educativa para incrementar el consumo de frijoles”, se realizó en una comunidad urbana costarricense. El grupo objetivo de la Campaña estuvo conformado por madres de niños escolares de clase media. Por su estrecho contacto con las madres los escolares entre 9 y 11 años, de tercero y cuarto grados, constituyeron el grupo interactivo. Con los escolares se realizaron una serie de actividades que tenían como objetivo motivarlos a consumir frijoles y capacitarlos para que pudieran contribuir a reforzar los mensajes que se dirigían al grupo objetivo de la Campaña. El presente estudio corresponde a una evaluación que se realiza a los escolares, alrededor de un año después de haberse iniciado la Campaña en la comunidad. Los resultados revelaron que un 73% de los escolares consumían más frijoles después de la Campaña. La mayoría lo hacía por convicción propia y dieron razones que pueden asociarse directamente a la Campaña. Esto refleja un efecto positivo sobre sus actitudes, que resulta importante para la modificación de hábitos alimentarios. Con respecto a conocimientos, un 100% de los escolares reconocía los frijoles como beneficiosos para la salud y la mayoría los asoció con razones nutricionales. En general, la confrontación de los resultados obtenidos con los “indicadores de éxito” fijados en el Plan de Comunicación de la Campaña, reveló efectos positivos de la Campaña en la población escolar.

  3. PRODUCTIVIDAD Y RENTABILIDAD DEL CULTIVO DE FRIJOL CON FERTIRRIEGO EN VERACRUZ, MÉXICO

    OpenAIRE

    2011-01-01

    El objetivo de este trabajo fue determinar el efecto del fertirriego en el cultivo de frijol en las zonas centro y sur del estado de Veracruz, México. Durante los ciclos invierno-primavera 2000 y 2001 se evaluaron tres tratamientos: 1) Riego rodado (por gravedad) y fertilización sólida manual (testigo regional), con la dosis 40N-40P-0K (RR-40), 2) Riego por goteo y fertilización sólida manual, con la dosis 4N-40P-0K (RG-40); en ambos tratamientos se aplicó el fertilizante a los quince días de...

  4. Relaciones hídricas y temperaturas altas en frijol del tipo "flor de mayo"

    OpenAIRE

    2010-01-01

    Se estudió el efecto del déficit hídrico del suelo y temperatura alta del aire en las relaciones hídricas y temperatura del dosel del cultivo (Tc), rendimiento de semilla (RS) y sus componentes en 8 variedades de frijol del tipo ?Flor de Mayo? (FM) y una variedad criolla. Se realizaron 3 experimentos de campo; Montecillo en riego (MR) con la aplicación de agua de la siembra a la madurez, Montecillo en secano (MS) con la lluvia como fuente única de humedad y Celaya en secano (CS) con lluvia y ...

  5. Location and Shallow Structure of the Frijoles Strand of the San Gregorio Fault Zone, Pescadero, California

    Science.gov (United States)

    Fox-Lent, C.; Catchings, R. D.; Rymer, M. J.; Goldman, M. R.; Steedman, C. E.; Prentice, C. S.

    2003-12-01

    The San Gregorio fault is one of the principal faults of the San Andreas fault system in the San Francisco Bay area. Located west of the active trace of the San Andreas fault and near the coast, the San Gregorio fault zone consists of at least two northwest-southeast-trending strands, the Coastways and Frijoles faults. Little is known about the slip history on the San Gregorio, and information for the Frijoles fault is especially scarce, as it lies mostly offshore. To better understand the contribution of the San Gregorio fault zone to slip along the San Andreas fault system, we conducted a high-resolution, seismic imaging investigation of the Frijoles fault to locate near-surface, onshore, branches of the fault that may be suitable for paleoseismic trenching. Our seismic survey consisted of a 590-meter-long, east-west-trending, combined seismic reflection and refraction profile across Butano Creek Valley, in Pescadero, California. The profile included 107 shot points and 120 geophones spaced at 5-m increments. Seismic sources were generated by a Betsy Seisgun in 0.3-m-deep holes. Data were recorded on two Geometrics Strataview RX-60 seismographs at a sampling rate of 0.5 ms. Seismic p-wave velocities, determined by inverting first-arrival refractions using tomographic methods, ranged from 900 m/s in the shallow subsurface to 5000 m/s at 200 m depth, with higher velocities in the western half of the profile. Migrated seismic reflection images show clear, planar layering in the top 100-200 meters on the eastern and western ends of the seismic profile. However, to within the shallow subsurface, a 200-m-long zone near the center of the profile shows disturbed stratigraphic layers with several apparent fault strands approaching within a few meters of the surface. The near-surface locations of the imaged strands suggest that the Frijoles fault has been active in the recent past, although further paleoseismic study is needed to detail the slip history of the San Gregorio

  6. Efecto de la cocción sobre algunas características nutricionales del frijol

    OpenAIRE

    2016-01-01

    Se estudiaron siete variedades de frijol de los tipos: Flor de Mayo, Negro, Bayo y Canario, además de la línea BAT 104. El objetivo del presente estudio fue analizar las características tecnológicas y nutricionales, así como los cambios que por efecto de la cocción resultan en la digestibilidad de la proteína y la actividad del inhibidor de tripsina en el grano de los materiales. Se encontraron diferencias significativas entre variedades en el peso, tamaño, porcentaje de testa, tiempo de cocc...

  7. Adopción de variedades de frijol en el noroeste de Zacatecas

    OpenAIRE

    Guillermo Galindo-González; Román Zandate-Hernández

    2006-01-01

    En el noroeste de Zacatecas, en el primer semestre de 2003, se realizó un estudio que tuvo como objetivo conocer el grado de adopción de 11 variedades de frijol en condiciones de temporal. Estas variedades fueron: Negro Durango, Negro Altiplano, Negro 8025, Negro Vizcaya, Negro Otomí, Flor de Mayo 2000, Flor de Junio Victoria, Negro Ontiveros, Pinto Bayacora, Flor de Mayo Sol y Flor de Mayo M-38. Las variedades mencionadas se difundieron por medio del establecimiento de parcelas demostrativas...

  8. Efecto de labranza, humedad y fertilización en el rendimiento de frijol y la patogenicidad de Macrophomina phaseolina (Tassi Goid Effects of tillage, moisture and fertilization on the yield of the common bean and the pathogenicity of Macrophomina phaseolina (Tassi Goid

    Directory of Open Access Journals (Sweden)

    Krystal Lira-Méndez

    2012-04-01

    Full Text Available El hongo Macrophomina phaseolina (Tassi Goid. causa la 'pudrición carbonosa' en frijol común (Phaseolus vulgaris L. y otros cultivos y afecta el crecimiento y el rendimiento de grano. En este trabajo se determinó el efecto de cuatro niveles de labranza (barbecho, destronque-bordeo, subsuelo-bordeo, cero, dos de humedad (temporal, riego y dos de fertilización (química, 40-20-00 NPK; biológica con la micorriza Glomus intraradices en el rendimiento de grano de la variedad Negro INIFAP en 2006 y 2007 en Río Bravo, México y en la patogenicidad de M. phaseolina. Los experimentos se establecieron en arreglo de tratamientos en parcelas subdivididas con tres repeticiones. Un aislamiento de M. phaseolina se obtuvo de plantas colectadas en cada unidad experimental y su patogenicidad se midió en semillas de cinco variedades de frijol (Azufrado Tapatío, Bayo Madero, Flor de Mayo Bajío, Negro Altiplano y Negro INIFAP. El riego y G. intraradices incrementaron el rendimiento de grano 144% y 12% respecto al temporal y la fertilización química, respectivamente. Los aislamientos de M. phaseolina de parcelas con riego, fertilización química, subsuelo-bordeo y barbecho fueron más agresivos en frijol. La aplicación de micorrizas y la labranza de conservación promueven la sostenibilidad del suelo y también podrían constituirse en medidas de manejo integrado de M. phaseolina en frijol.The fungus Macrophomina phaseolina (Tassi Goid causes the 'charcoal rot' disease in common beans (Phaseolus vulgaris L. and other crops and reduces plant growth and grain yield. In this work we determined the effects of four tillage levels (moldboard plow, shred-bedding, subsoil-bedding, no or zero tillage; two levels of soil moisture (rainfed, irrigated and two of fertilization (chemical, using the formula 40-20-00 NPK; biological, using the mycorrhiza Glomus intraradices in grain yield of the variety Negro INIFAP during 2006 and 2007 in Río Bravo, Mexico as well as

  9. Experiencias y aprendizajes del desarrollo de variedades de frijol de manera participativa en el norte de Nicaragua

    National Research Council Canada - National Science Library

    Conny Almekinders; Julio Molina-Centeno; Rolando Herrera-Torrez; Santos Luis Merlo-Olivera; José Manuel González-Suárez; Juan García-Carrasco

    2006-01-01

    .... Por medio de selección dentro y entre familias, cada agricultor desarrolló una línea avanzada de frijol con resistencia al Mosaico Dorado adaptado a sus condiciones de producción y preferencias. La comparación y validación...

  10. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus that causes variegatio

  11. Chlorotic mottle of bean (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Jayasinghe, W.U.

    1982-01-01

    For the past years there have been outbreaks of a disease of bean ( Phaseolus vulgaris L.) in Colombia called bean chlorotic mottle. The etiology of bean chlorotic mottle was not known, but the disease was generally believed to be incited by the same whitefly-transmitted virus

  12. Mejoramiento genético del frijol rojo y negro mesoamericano para Centroamérica y El Caribe

    Directory of Open Access Journals (Sweden)

    Juan C. Rosas

    2000-01-01

    Full Text Available La producción de frijol en Centroamérica es llevada a cabo mayormente en laderas de suelos marginales, limitada por diversos factores bióticos y abióticos. Más de 350,000 t de este grano se producen en cerca de 0,5 millones de hectáreas, con rendimientos promedios de 678 kg/ha. La mayoría de los productores de frijol utilizan bajos insumos y muy pocas fincas están mecanizadas. El autoconsumo es alto; sin embargo, una alta proporción de este grano es comprado por intermediarios. En Honduras, el frijol es el sétimo cultivo en valor económico y es el de mayor rentabilidad entre el maíz, arroz y sorgo. Las dos clases comerciales más importantes en Centroamérica son el frijol rojo y negro pequeños (raza Mesoamérica. El mejoramiento de estos tipos de frijol se ha enfocado en el desarrollo de resistencia a enfermedades (mosaico común, mosaico dorado, antracnosis, mancha angular, roya, mustia hilachosa y bacteriosis común e insectos (principalmente picudo de la vaina; y tolerancia a baja fertilidad, sequía y calor. Programas adecuados de hibridación, evaluación y selección son usados para el desarrollo de cultivares con resistencia múltiple, alto rendimiento, buena adaptación y aceptación comercial. La ampliación de la base genética se obtiene usando fuentes de germoplasma Andino y Mesoamericano en la etapa de hibridación. La selección simultánea por varios caracteres y pruebas multilocalidades son utilizadas. Los viveros y ensayos de adaptación y rendimiento de líneas avanzadas son distribuídos a países de Centroamérica, México, y El Caribe. Las pruebas y validaciones en fincas y la liberación de variedades, son efectuadas por los programas nacionales de frijol de los países miembros de PROFRIJOL, en colaboración con el Zamorano, CIAT y el Bean/Cowpea CRSP

  13. Ecofisiología de seis variedades de frijol bajo las condiciones climáticas de la Región Lagunera Ecophysiology of six bean varieties under the weather conditions of the Lagunera region

    Directory of Open Access Journals (Sweden)

    Jorge Armando Chávez-Simental

    2012-04-01

    Full Text Available En la problemática existente en la Comarca Lagunera sobre la disponibilidad de agua, es importante considerar cultivos alternos que se adapten a las condiciones climáticas de la región. Algunas variedades de frijol (Phaseolus vulagris L. son susceptibles a sequía lo que limita el óptimo desarrollo del cultivo provocando bajo rendimiento. El objetivo de este trabajo fue determinar la tasa de fotosíntesis neta, tasa de transpiración, conductancia estomatal y rendimiento de seis variedades de frijol así como su adaptabilidad a las condiciones climáticas de la Comarca Lagunera. La investigación se realizó en el campo experimental de la UAAAN Unidad Laguna durante el ciclo de otoño-invierno de 2009 utilizando riego tradicional. Se midió la tasa de fotosintética, tasa de transpiración y conductancia estomatal con el equipo de medición "photosynthesis system CI 340®". Además, se evaluó el rendimiento el cual fue correlacionado con las funciones fisiológicas antes mencionadas. La variedad que pre sentó el mayor rendimiento fue la Pinto Villa, la cual también mostró la mayor actividad fotosintética ubicándose dentro del grupo de genotipos con la conductancia estomatal más alta. Por otra parte, la variedad Pinto Mestizo obtuvo el segundo mejor rendimiento, aunque la actividad fisiológica de las variables estudiadas fueron las más bajas. Por lo tanto, las variedades Pinto Villa y Pinto Mestizo fueron las que mejor adaptabilidad presentaron a las condiciones climáticas de la Región Lagunera.In the current situation in the Comarca Lagunera regarding water availability, it is important to consider alternate crops that adapt to the area's weather conditions. Some bean varieties (Phaseolus vulagris L. are susceptible to drought, which limits the optimum crop development, causing low yields. The main objective of this study was to determine the net photosynthesis rate, transpiration rate, stomatal conductance, and the yield of six

  14. Evaluación de la Disponibilidad de Minerales en Harinas de Frijol y en Mezclas de Maíz/Frijol Extrudidas Evaluation of Mineral Availability in Cowpea Flour and in Maize/Cowpea Extruded Mixtures

    Directory of Open Access Journals (Sweden)

    Silvina R Drago

    2007-01-01

    Full Text Available En el presente trabajo, se analizaron los efectos de la extrusión sobre la disponibilidad de minerales, utilizando mezclas de maíz y frijol (con y sin tratamiento previo de inactivación. Al formular alimentos destinados a programas alimentarios se debe considerar el contenido y la calidad proteica y la biodisponibilidad de nutrientes. Una alternativa explorada en este trabajo es utilizar harinas de frijol, asegurando la calidad organoléptica del producto mediante tratamientos de inactivación de lipooxigenasa. La disponibilidad de los minerales se estimó a través del porcentaje dializado tras la digestión in vitro. Los resultados mostraron que las mezclas maíz/frijol presentaron una mejora significativa de la cantidad y calidad proteica, con respecto al maíz. La inactivación no afectó la dializabilidad de hierro en la harina de frijol, mientras que la extrusión la mejoró. Si bien ambos procesos tuvieron un ligero efecto desfavorable en la dializabilidad del zinc, el valor de ésta fue bastante altoIn the present work the effects of extrusion on mineral availability of extruded mixtures based on maize and cowpea (with and without previous inactivation treatment were analyzed. In the formulation of foods aimed to food programs the amount and the quality of protein and bioavailability of the nutrients must be considered. One alternative, explored in this work, is to use bean flours, assuring the organoleptic quality, by lipoxigenase inactivation treatments. Mineral availability was estimated as the percentage of dialyzed mineral after in vitro digestion. Results showed that maize/cowpea mixture had a significant improvement in the amount of protein and its quality in comparison with maize. The inactivation treatment did not affect iron dialyzability of the cowpea flour while extrusion increased it. Although both process moderately impaired zinc dialyzability, the values were still high

  15. Efecto de la sequía en la producción de biomasa y grano de frijol Drought effect in bean biomass and grain production

    Directory of Open Access Journals (Sweden)

    Efraín Acosta-Díaz

    2011-04-01

    Full Text Available La sequía intermitente es el factor que más limita la producción de frijol (Phaseolus vulgaris L. bajo temporal en México. El objetivo fue determinar el efecto de la sequía sobre la acumulación de biomasa del vástago y el rendimiento de grano en variedades criollas e introducidas de frijol de temporal. Se establecieron dos experimentos, uno con 21 variedades nativas y otro con 15 variedades introducidas, de diferente hábito de crecimiento indeterminado, en la Unidad Académica La Ascensión de la Facultad de Agronomía de la Universidad Autónoma de Nuevo León, México en 2009. Los experimentos se condujeron bajo dos tratamientos de humedad del suelo: riego durante todo el ciclo y sequía. En el tratamiento de riego el suelo se mantuvo por encima de 60% de humedad aprovechable durante el ciclo del cultivo, mediante la precipitación y cuatro riegos de auxilio (dos antes y otros dos después de floración, mientras que en el tratamiento de sequía el riego se suspendió a partir del inicio de la floración. En los dos experimentos, bajo la condición de sequía la acumulación de biomasa del vástago y el rendimiento de grano fue significativamente inferior (p≤ 0.01 que en el tratamiento de riego. En general, el efecto del tratamiento de sequía sobre el peso seco del vástago fue mayor en las variedades nativas, mientras que el efecto sobre el rendimiento de grano fue mayor en las variedades introducidas. En sequía, las variedades de mayor rendimiento, fueron: nativas, Pinto-2, Pinto-1, Amarillo Mantequilla, Boleado, Bayo Blanco, Canelo y Quipincillo Rojo-1 e introducidas, Peruano, Flor de Mayo Media Oreja, Pinto-5 y Ojo de Cabra. De acuerdo con el índice de susceptibilidad a la sequía y la media geométrica, las variedades más eficientes para la producción de rendimiento de grano en ambas condiciones de humedad fueron: Pinto-2, Amarillo Mantequilla, Pinto-1, Boleado, Peruano, Flor de Mayo Media Oreja, Pinto-5 y Ojo de Cabra; sin

  16. Detección por PCR de Colletotrichum lindemuthianum en cultivos y semillas de frijol en Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Leonardo Martínez Pacheco

    2014-12-01

    Full Text Available Colletotrichum lindemuthianum, agente causal de la antracnosis del frijol, es uno de los patógenos más limitantes en la producción de este cultivo. La detección y correcta identificación de este hongo resulta fundamental para el manejo de la enfermedad, siendo las pruebas moleculares alternativas rápidas y sensibles para este fin. Mediante la técnica de PCR se evaluaron cuatro juegos de cebadores (CY1/CY2, CD1/CD2, ClF4/ITS4 y ClF432/ClR533 para la detección de C. lindemuthianum a partir de tejidos foliares, de vainas y de semillas procedentes de cultivos de frijol de Antioquia, Colombia. Los resultados indicaron que el par CD1/CD2, dirigido al pseudogen de permeasa de hierro Ftr1, fue el más efectivo para detectar el hongo en tejidos y semillas de frijol, así como para identificar aislamientos en cultivos microbiológicos. Para los cebadores CY1/CY2, dirigidos a los ITS del rDNA, se recomienda un esquema de PCR-RFLPs con MseI (=Tru1I para la diferenciación con las especies C. orbiculare y C. trifolii. Estos cebadores generaron resultados consistentes cuando se utilizaron en combinación con ITS1 (ITS1/CY2 e ITS4 (CY1/ITS4. Finalmente, los cebadores ClF4/ITS4 resultaron en amplificaciones inespecíficas y ClF432/ClR533 en fragmentos de difícil resolución en electroforesis de agarosa. Este estudio servirá de apoyo para los programas de certificación de semilla y mejoramiento genético de frijol.

  17. Efecto de rastrojos de malezas y herbicidas pre-emergentes en el control de malezas en frijol

    Directory of Open Access Journals (Sweden)

    Franklin Herrera

    2000-01-01

    Full Text Available En la Estación Experimental Fabio Baudrit, Alajuela, Costa Rica de setiembre de 1998 a febrero de 1999, se evaluó el efecto de los rastrojos de Ixophorus unisetus, Digitaria spp., Cynodon dactylon y Rottboellia cochinchinensis sobre: las malezas, la eficacia de los herbicidas pendimetalina, alaclor e imazetapir, y sobre la nodulación por Rhizobium y el crecimiento del frijol. Se encontró que la presencia de rastrojos sobre la superficie del suelo a razón de 4,5 t de materia seca/ha no fue efectiva para reducir el crecimiento de malezas en el cultivo del frijol sembrado en labranza de conservación. La eficacia de los herbicidas pendimetalina, imazetapir y alaclor no fue afectada por la presencia de los rastrojos sobre la superficie del suelo. Los rastrojos de Ixophorus unisetus afectaron negativamente el crecimiento y la producción del frijol, mientras que los rastrojos de Digitaria spp. y Cynodon dactylon tuvieron efectos positivos sobre el cultivo

  18. Advances in the improvement of tepary bean (Phaseolus acutifolius)

    Science.gov (United States)

    Climate change, high temperature and drought are increasingly critical factors affecting agriculture and specifically the production of common bean (Phaseolus vulgaris L.). Tepary bean (Phaseolus acutifolius A. Gray), native to the Sonora desert located in the northern part of Mexico and southwest o...

  19. Onderzoekingen over virusziekten van de boon (Phaseolus vulgaris L.)

    NARCIS (Netherlands)

    Want, van der J.P.H.

    1954-01-01

    Three viruses were studied which produce diseases in French beans, Phaseolus virus I (PV1), Phaseolus virus 2 (PV2) and a virus isolated from white clover (WKV). Included are symptoms, host plants, properties in vitro, occurrence and spread in the field. Special attention

  20. Las arvenses y su entomofauna asociada en el cultivo del frijol (Phaseolus vulgaris, L. posterior al periodo crítico de competencia

    Directory of Open Access Journals (Sweden)

    Y. Blanco

    2013-01-01

    Full Text Available Se analizaron los costos en que se incurren y los beneficios económicos que se generan en un SSPi con base en Leucaena leucocephala, en terrenos de riego por gravedad, con el fin de evaluar la rentabilidad económica de las inversiones y hacer una comparación entre el SSPi y el sistema que tradicionalmente operaba o línea base (40% monocultivo de pasto Tanzania Panicum maximum, 30% de sorgo forrajero Sorghum vulgar bajo el sistema de corte y 30% de agostadero con pastos nativos. Complementando la alimentación del ganado suizo de doble propósito en el SSPi con pulido de arroz a razón de 1.50 kg diarios por vaca durante la ordeña; y en la línea base se utilizó 3.50 kg de alimento comercial diario por vaca ordeñada. Resultando, para el SSPi, una TIR del 13.30% y el VAN al 10%, un saldo positivo de $2’202,170 (pesos mexicanos y en la situación inicial o línea base se obtuvo una TIR del 0.70% y el VAN al 10% resultó negativo, con $-4’717,022 (pesos mexicanos. El SSPi incrementó de manera importante la productividad y la rentabilidad del rancho haciéndolo lucrativo, ya que con el sistema tradicional no era rentable económicamente.

  1. Determinación de la capacidad de inducción de callo de dos variedades de frijol Phaseolus vulgaris L. en dos medios de cultivo (ING

    Directory of Open Access Journals (Sweden)

    Jorge Madriz Muñoz.

    2016-03-01

    Full Text Available The cotiledon, epicotyl and leaf callogenesis capacity was studied on the bean commercial varieties Huasteco and Huetar cultivated on Fj and PC-L2 media. The three explant types from the cultivar Huasteco formed callus on both tested media. The Huetar explants formed callus only on the PC-L2 medium; however, the cotiledon explant formed callus on Fj medium. The highest accumulated relative growth index was observed at the end of the first and second week. One week later (end of third week, this parameter varied among explants, cultivars and culture media, showing a decreasing tendency at the fourth week.

  2. Evaluación de la fijación de nitrógeno por cepas de Rhizobium que nodulan Frijol (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    María Inés Ballesteros

    2010-07-01

    Full Text Available La capacidad de fijar nitrógeno de ocho cepas de Rhizohium fue evaluada incdiante dos ensayos de invernadero, en solución nutritiva, con cinco variedades de fríjol: ICA CITARA, ICA PIJAO, DIACOL CATIO, ANDINO y PVA 916. Se determinó en floración el peso seco de la parle aérea, peso seco de nodulos y nitrógeno total en la planta por el método de microkjeldahi. Las cepas de mejor comportamiento en cuanto a nitrógeno fijado fueron en su orden : CIAT 144, CIAT 899, y CIAT 2; aunque en general la respuesta de las cepas fue diferente con la variedad de Iríjol se logró un porcentaje promedio de nitrógeno fijado del orden del 28% comparado con los controles que recibieron nitrógeno combinado (Ensayo 2. Las cepas CIAT 71 15 y CIAT 632 presentaron la menor efectividad con las variedades ensayadas. Se encontró correlación altamente significativa entre el rendimiento en peso seco y el nitrógeno fijado para todas las variedades de fríjol.

  3. Efecto de aislados de los géneros Streptomyces y Bacillus como promotores de crecimiento vegetal en frijol (Phaseolus vulgaris L.)

    OpenAIRE

    López Soto, Nataly

    2014-01-01

    El uso de bacterias promotoras del crecimiento vegetal o PGPR (por sus siglas en inglés Plant Growth Promoting Rhizobacteria) representa una estrategia biotecnológica sostenible para contrarrestar los efectos detrimentales del uso exagerado de fertilizantes químicos y pesticidas en la agricultura, ya que otorgan beneficios a las plantas como la movilización de nutrientes y la producción de reguladores del crecimiento vegetal, entre otros. Los géneros de bacterias Streptomyces y Bacillus se de...

  4. Efecto del estado de desecación del rastrojo de arroz en las malezas y en el rendimiento del frijol

    Directory of Open Access Journals (Sweden)

    Franklin Herrera

    2003-01-01

    Full Text Available En la Estación Experimental Agrícola Fabio Baudrit Moreno de la Universidad de Costa Rica, ubicada en el distrito San José, provincia de Alajuela, en la zona de vida Bosque Húmedo Premontano y, entre junio del 2000 y enero del 2001, se realizó un experimento con el propósito de medir el efecto de desecar rastrojo de arroz con glifosato en diferentes periodos antes de la siembra directa del frijol, en el combate de malezas y en el rendimiento del frijol. La aplicación de glifosato sobre los rastrojos de arroz y malezas, desde cinco días antes de la siembra del frijol, hasta dos días después de la siembra pero antes de la emergencia, permitió una adecuada cobertura del suelo por los rastrojos durante el periodo crítico de competencia del frijol, con lo cual se redujo la presencia de malezas y se logró obtener altos rendimientos de frijol sin necesidad de aplicar otros herbicidas. Cuando el periodo de espera después de la aplicación del glifosato fue muy prolongado (30-45 días, la cobertura del suelo por el rastrojo fue pobre, debido a su avanzado estado de descomposición, por lo que hubo mayor presencia de malezas y efectos negativos en la producción de frijol, cuando no hubo control oportuno de esas malezas.

  5. Mejora genética participativa de la variedad criolla de frijol "Sacapobres"

    Directory of Open Access Journals (Sweden)

    Rodolfo Araya-Villalobos

    2006-01-01

    Full Text Available Agricultores experimentadores de la Región Brunca de Costa Rica solicitaron en el año 1999, al Programa de Investigación y Transferencia de Tecnología Agropecuaria en frijol (PITTA Frijol, la mejora genética de la variedad criolla Sacapobres, una de las principales variedades criollas de la Región Brunca, que posee precocidad (60 días a cosecha, superando en más de diez días a la variedad mejorada más precoz y alto potencial de rendimiento en grano (no había podido ser sustituida por las variedades mejoradas en sus campos de producción, pero de alta emisión de guías y acame. En el Centro Internacional de Agricultura Tropical (CIAT y la Escuela Agrícola Panamericana Zamorano, se efectuaron hibridaciones con esta variedad criolla. La selección de los materiales segregantes se inició en los años 2000 y 2001, en Costa Rica y se basó en la metodología de fitomejoramiento participativo (FP. Los sitios de selección fueron fincas de agricultores y una estación experimental. El principal criterio empleado por los agricultores fue la arquitectura erecta (ausencia o poca emisión de guías y sin acame para facilitar el tránsito por el terreno en las labores de deshierba y fumigación, seguido del tipo, tamaño y color de grano e incidencia de patógenos. Se seleccionaron, en el año 2005, dos líneas promisorias, que pasarán a la etapa de validación. Se describe la estrategia de selección en FP, la genealogía de los progenitores empleados y se concluye sobre el futuro mejoramiento de Sacapobres

  6. Diversidad de frijoles nativos de diferentes regiones del estado de Puebla Native beans diversity of different regions from state of Puebla

    OpenAIRE

    Ana Rosa Ramírez-Pérez; Ramón Díaz-Ruiz; Carmen Jacinto-Hernández; Juan Alberto Paredes-Sánchez; Ramón Garza García

    2012-01-01

    El frijol común representa un cultivo básico en México con una gran variabilidad en sus características morfológicas. En estudios sobre la diversidad genética se han usado marcadores moleculares, entre los que se encuentran los RAPD. El presente trabajo tuvo como objetivo caracterizar mediante caracteres morfológicos, agronómicos, de calidad y marcadores RAPD, variedades de frijoles nativos cultivadas en diferentes comunidades del estado de Puebla. La extracción de ADN se realizó en una muest...

  7. Variación del tamaño de frutos y semillas en 38 poblaciones silvestres de Phaseolus lunatus (Fabaceae del Valle Central de Costa Rica

    Directory of Open Access Journals (Sweden)

    Elida M Vargas

    2003-09-01

    Full Text Available Se estudió la diversidad morfológica de frutos y semillas de 38 poblaciones silvestres de Phaseolus lunatus var. lunatus (frijol lima en el Valle Central de Costa Rica. Para ello se determinó la variación en el largo y ancho de vaina, el largo, ancho y grosor de las semillas, la razón entre estas variables y el peso promedio de 100 semillas. En términos generales, se puede afirmar que existen diferencias significativas entre las poblaciones analizadas para todas estas variables. Cuando se agruparon las 38 poblaciones en ocho regiones geográficas dentro del área de estudio, se encontró que existían diferencias significativas entre distintas regiones, pero la variación entre las poblaciones de cada región era mayor que aquella encontrada entre regiones. Estos resultados sugieren que no existe asociación entre estas variables y la región geográfica. Se analizó la utilidad de estos resultados para el desarrollo de estrategias para la conservación in situ de las poblaciones silvestres de Phaseolus lunatusWe studied the morfological diversity in fruits and seeds in 38 wild populations of Phaseolus lunatus var. lunatus (lima beans in the central valley of Costa Rica. In order to do so, measured the lenght and width of the fruits and the lenght, width and thickness of seeds. We also calculated the ratio between these traits and determined the weight of 100 seeds. In general, we found significant variation between populations for all variables. When we grouped the 38 populations into eight geographical regions within the study area, we found significant differences between regions. However, the levels of variation between populations within geographical regions was larger than that found between geographical regions. These findings suggested that there is no clear relationship between these variables and the geographical grouping established in this study. The implications of these findings for the establishment of strategies for in situ

  8. 78 FR 73886 - Atmel Corporation, Colorado Springs, Colorado; Amended Certification Regarding Eligibility To...

    Science.gov (United States)

    2013-12-09

    ... Employment and Training Administration Atmel Corporation, Colorado Springs, Colorado; Amended Certification... Corporation, Colorado Springs, Colorado. The Department's notice of determination was published in the Federal... workers at Atmel Corporation, Colorado Springs, Colorado were engaged in activities related to...

  9. Producción artesanal de semilla de frijol con participación municipal en Veracruz, México.

    OpenAIRE

    2014-01-01

    El objetivo de este trabajo fue evaluar la producción artesanal de semilla de frijol municipal (PASF-Municipal). La estrategia se implementó de 1998-2010, en veintitrés municipios de las regiones Centro-sur y de las Altas Montañas del estado de Veracruz, México. El estudio constó de dos fases: una de establecimiento de parcelas de validación de variedades para seleccionar las de mayor productividad; la segunda fase fue la de instalación de parcelas de producción de semilla de las variedades m...

  10. PRODUCCIÓN ARTESANAL DE SEMILLA DE FRIJOL CON PARTICIPACIÓN MUNICIPAL EN VERACRUZ, MÉXICO

    OpenAIRE

    2014-01-01

    El objetivo de este trabajo fue evaluar la producción artesanal de semilla de frijol municipal (PASF-Municipal). La estrategia se implementó de 1998-2010, en veintitrés municipios de las regiones Centro- sur y de las Altas Montañas del estado de Veracruz, México. El estudio constó de dos fases: una de establecimiento de parcelas de validación de variedades para seleccionar las de mayor productividad; la segunda fase fue la de instalación de parcelas de producción de semilla de las varie...

  11. ACE-I Inhibitory Activity from Phaseolus lunatus and Phaseolus vulgaris Peptide Fractions Obtained by Ultrafiltration.

    Science.gov (United States)

    Betancur-Ancona, David; Dávila-Ortiz, Gloria; Chel-Guerrero, Luis Antonio; Torruco-Uco, Juan Gabriel

    2015-11-01

    The involvement of angiotensin-I-converting enzyme (ACE-I) as one of the mechanisms controlling blood pressure is being studied to find alternative means of control of hypertension on human beings. On the market there are synthetic drugs that can control it, but these can cause undesirable health side effects. In this work was assessed the fractionation by ultrafiltration of the Lima bean (Phaseolus lunatus) and Jamapa bean (Phaseolus vulgaris), protein hydrolysates obtained with Alcalase(®) and Flavourzyme(®) on ACE-I inhibitory activity. Four membranes of different molecular cutoffs (10, 5, 3, and 1 kDa) were used. Fractions that had a higher inhibitory activity in both legumes were denominated as E (obtaining and utilizing these peptide fractions in the development and innovation of a functional product that helps with treatment and/or prevention of hypertension.

  12. Galactooligosaccharides of seeds during growth of Phaseolus coccineus L. and Phaseolus vulgaris L. beans

    Directory of Open Access Journals (Sweden)

    Ryszard Kosson

    2013-12-01

    Full Text Available The quantitative changes of raffinose and stachyose in seeds of Phaseolus coccineus L. and Phaseolus vulgaris L. during their growth and maturation in a two year experiment were investigated. Trace amounts of raffinose were found in Ph. vulgaris seeds during their vegeta tive growth in 1990. Time of raffinose accumulation in seeds in 1991 began not earlier than on 33rd day since inflorescence. Stachyose started to accumulate in seeds between 33rd and 47th day after inflorescence of all tested cultivars. It was noticed that stachyose and raffinose contents in seeds of most tested cultivars after ten years of storage did not differ significantly when compared to not stored ones.

  13. The influence of aluminium availability on phosphate uptake in Phaseolus vulgaris L. and Phaseolus lunatus L.

    Science.gov (United States)

    Mimmo, Tanja; Sciortino, Marco; Ghizzi, Massimiliano; Gianquinto, Giorgio; Gessa, Carlo E

    2009-01-01

    Aluminium toxicity is one of the major limiting factors of crop productivity on acid soils. High levels of available aluminium in soil may induce phosphorus deficiency in plants. This study investigates the influence of Aluminium (Al) on the phosphate (P(i)) uptake of two Phaseolus species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L. The two bean species were treated first with solutions of Al at different concentrations (0, 25, 50 and 100microM, pH 4.50) and second with solutions of P(i) (150microM) at pH 4.50. The higher the Al concentration the higher the Al concentration sorbed but P. vulgaris L var. Red Kidney adsorbed significantly more Al than P. lunatus L. Both species released organic acids: P. vulgaris L var. Red Kidney released fumaric acid and P. lunatus L. fumaric and oxalic acids which could have hindered further Al uptake. The two bean species showed a sigmoid P(i) uptake trend but with two different mechanisms. P. vulgaris L var. Red Kidney showed a starting point of 3h whereas P. lunatus L. adsorbed P(i) immediately within the first minutes. In addition, P. vulgaris L var. Red Kidney presented significantly higher P(i) uptake (higher uptake rate 'k' and higher maximum adsorption 'a' of the kinetic uptake model). The Al treatments did not significantly influence P(i) uptake. Results suggest that P. lunatus L. might adopt an external Al detoxification mechanism by the release of oxalic acid. P. vulgaris L var. Red Kidney on the other hand seemed to adopt an internal detoxification mechanism even if the Al sorbed is poorly translocated into the shoots. More detailed studies will be necessary to better define Al tolerance and/or resistance of Phaseolus spp.

  14. Phenotypic Diversity among Croatian Common Bean (Phaseolus vulgaris L.) Landraces

    National Research Council Canada - National Science Library

    Monika Vidak; Sara Malešević; Martina Grdiša; Zlatko Šatović; Boris Lazarević; Klaudija Carović-Stanko

    2016-01-01

    Phenotypic diversity among Croatian common bean (Phaseolus vulgaris L.) landraces was assessed by analysing 12 qualitative and six quantitative traits in 338 accessions collected from all production areas in Croatia...

  15. Dinámica de la producción de maíz y frijol en México de 1980 a 2014

    Directory of Open Access Journals (Sweden)

    Mauricio De los Santos Ramos

    2017-04-01

    Full Text Available El objetivo de este trabajo fue evaluar la dinámica de la producción de maíz y frijol en México. Se efectuó un análisis retrospectivo para identi car los puntos críticos, los cambios y ajustes dados en el sector como resultado de las políticas del gobierno mexicano. Los datos fueron obtenidos de SAGARPA, Banco de México, Secretaría de Economía y de la Organización de las Naciones Unidas para la Alimentación y la Agricultura. Se evaluaron indicadores de tasa de crecimiento media anual (TCMA, tendencia y precios constantes. Al realizarse el análisis nacional, se consideró como variable al país en su conjunto. Se analizaron los datos de superficie sembrada, producción, precios e importaciones de maíz y frijol en México entre los años 1980 a 2014. La superficie sembrada de maíz y frijol tuvo un decremento de 1,8 millones y 193 mil hectáreas, respectivamente; el rendimiento se incrementó para el maíz en 1,47 t/ha y en frijol 160 kg/ha, la producción de maíz tuvo una TCMA de 1,8% equivalente 10,1 millones de toneladas, el frijol tuvo una TCMA 0,89% con un incremento de 338 783 t. El maíz y frijol a precios constantes han tenido una disminución del 64,6% y 59,0%, correspondiente a 441,9 y 976,3 dólares por tonelada, respectivamente. Las importaciones de maíz tuvieron una TCMA de 2,8%, nalizó el 2012 con 9,5 millones de toneladas, las importaciones de frijol no mostraron ninguna tendencia. El punto crítico en las importaciones se dio con la entrada en vigor del Tratado de Libre Comercio de América del Norte, pasó de una economía cerrada a una abierta, donde se incrementaron más las importaciones que la producción nacional.

  16. Effects of processing on bean (Phaseolus vulgaris L.) protein quality.

    OpenAIRE

    Poel, van der, A.F.B.

    1990-01-01

    In animal production, feeding has an important impact on productivity and health of animals and feed composition is known to influence protein and energy metabolism directly. For monogastric animals complete diets are manufactured in which feed ingredients are used to supply the energy yielding and other nutrients. The common bean ( Phaseolus vulgaris L.) is such an ingredient.In common beans ( Phaseolus vulgaris L.) the supply of nutrients is often lower than is expected from its chemical an...

  17. Evolutionary dynamics of satellite DNA repeats from Phaseolus beans.

    Science.gov (United States)

    Ribeiro, Tiago; Dos Santos, Karla G B; Richard, Manon M S; Sévignac, Mireille; Thareau, Vincent; Geffroy, Valérie; Pedrosa-Harand, Andrea

    2017-03-01

    Common bean (Phaseolus vulgaris) subtelomeres are highly enriched for khipu, the main satellite DNA identified so far in this genome. Here, we comparatively investigate khipu genomic organization in Phaseolus species from different clades. Additionally, we identified and characterized another satellite repeat, named jumper, associated to khipu. A mixture of P. vulgaris khipu clones hybridized in situ confirmed the presence of khipu-like sequences on subterminal chromosome regions in all Phaseolus species, with differences in the number and intensity of signals between species and when species-specific clones were used. Khipu is present as multimers of ∼500 bp and sequence analyses of cloned fragments revealed close relationship among khipu repeats. The new repeat, named jumper, is a 170-bp satellite sequence present in all Phaseolus species and inserted into the nontranscribed spacer (NTS) of the 5S rDNA in the P. vulgaris genome. Nevertheless, jumper was found as a high-copy repeat at subtelomeres and/or pericentromeres in the Phaseolus microcarpus lineage only. Our data argue for khipu as an important subtelomeric satellite DNA in the genus and for a complex satellite repeat composition of P. microcarpus subtelomeres, which also contain jumper. Furthermore, the differential amplification of these repeats in subtelomeres or pericentromeres reinforces the presence of a dynamic satellite DNA library in Phaseolus.

  18. PRODUCTIVIDAD Y RENTABILIDAD DEL CULTIVO DE FRIJOL CON FERTIRRIEGO EN VERACRUZ, MÉXICO

    Directory of Open Access Journals (Sweden)

    Francisco Javier Ugalde-Acosta

    2011-01-01

    Full Text Available El objetivo de este trabajo fue determinar el efecto del fertirriego en el cultivo de frijol en las zonas centro y sur del estado de Veracruz, México. Durante los ciclos invierno-primavera 2000 y 2001 se evaluaron tres tratamientos: 1 Riego rodado (por gravedad y fertilización sólida manual (testigo regional, con la dosis 40N-40P-0K (RR-40, 2 Riego por goteo y fertilización sólida manual, con la dosis 4N-40P-0K (RG-40; en ambos tratamientos se aplicó el fertilizante a los quince días de nacido el cultivo, y 3 riego por goteo y fertilización soluble, con la dosis 60N-60P-60K, aplicada en forma fraccionada durante el ciclo del cultivo (RG-60. De 2002 a 2005 se validó el mejor tratamiento y se realizó análisis económico para determinar su rentabilidad en comparación con el testigo regional. En la etapa de evaluación, con el tratamiento RG- 60 se redujo en 85% el gasto de agua y se obtuvo el mayor rendimiento promedio (2256 kg/ha, que superó en 145% al obtenido con RG-40 y en 186% al RR-40 (testigo regional. En la etapa de validación con riego por goteo y fertilización soluble fraccionada 60N-60-P-60K se obtuvo 110% más rendimiento que el testigo regional y mejor relación beneficio/ costo (1:1,80.

  19. Nomenclatura de variedades de frijol común liberadas en Centro América y El Caribe

    Directory of Open Access Journals (Sweden)

    Juan Carlos Rosas

    2004-01-01

    Full Text Available Nomenclatura de variedades de frijol común liberadas en Centro América y El Caribe. En los últimos años varias líneas mejoradas de frijol común han sido liberadas como variedades en diferentes países de Centro América y El Caribe. Los programas nacionales e instituciones involucradas en los procesos de liberación han asignado estas variedades con diferentes nombres, dando como resultado que algunas de ellas sean conocidas con un nombre diferente en cada país donde se le ha liberado. A corto plazo, esta situación podría causar confusiones en la identificación de estas variedades mejoradas actualmente utilizadas por los agricultores. El propósito de este documento es servir de referencia para guiar a investigadores, productores, distribuidores de semilla, funcionarios de gobierno y comercializadores, en sus decisiones con respecto al uso de estas variedades mejoradas para producción comercial de grano o semilla, su procesamiento industrial y mercadeo en la región.

  20. Evaluación de líneas de frijol negro, en Veracruz y Chiapas, México

    Directory of Open Access Journals (Sweden)

    Ernesto Lopez

    2001-01-01

    Full Text Available Evaluación de líneas de frijol negro, en Veracruz y Chiapas, México. El presente estudio se llevó a cabo durante los ciclos agrícolas de 1999-2000 y 2000-2001; se evaluó la adaptación y rendimiento de 15 líneas avanzadas de frijol negro, así como un testigo local. Dichos genotipos forman parte al Ensayo Centroamericano de Adaptación y Rendimiento (ECAR. La evaluación se realizó en 11 localidades, cinco en el estado de Chiapas y seis en Veracruz, los experimentos se establecieron bajo condiciones de riego, temporal y humedad residual. Las líneas ICTA-Ju 97-1, ICTA-Ju II- 307, DOR-678 y Cut-45, resultaron sobresalientes por su rendimiento, amplia adaptación y estabilidad. En el ciclo agrícola de otoño-invierno 1999-2000, se evaluó la respuesta de las líneas a las siguientes enfermedades: mosaico dorado (BGMV, mancha angular (Phaseoisariopsis griseola, roya (Uromyces appendiculatus y la antracnosis (Colletotrichum lindemuthianum. Las líneas que resultaron tolerantes fueron ICTA Ju 97-1 y Cut-45. Ambas serán validadas en campos de agricultores para su posible liberación como nuevas variedades para algunas regiones productoras de Veracruz y Chiapas, México

  1. CARACTERIZACIÓN PRELIMINAR CON MICROSATÉLITES DE LA COLECCIÓN GUATEMALTECA DE FRIJOL COMÚN TREPADOR

    Directory of Open Access Journals (Sweden)

    Karla Melina Ponciano-Samayoa

    2009-01-01

    Full Text Available El objetivo de este estudio fue caracterizar con microsatélites una colección de 558 accesiones de frijol voluble guatemalteco. En el Instituto de Ciencia y Tecnología Agrícolas (ICTA, en el periodo enero 2008 a junio 2009, con base en seis marcadores microsatélites, se analizaron agrupamientos y diversidad genética dentro de los grupos de frijol. Todos los loci analizados fueron polimórficos, se detectaron 25 alelos con un promedio de 4,12 alelos por marcador. El análisis de similaridad detectó genotipos duplicados que al ser eliminados dio lugar a una colección reducida de 261 muestras diferentes (46,8 % de la original. Los análisis de similaridad y correspondencia identificaron 12 grupos, incluyendo un control andino. La diversidad genética de Nei para la colección reducida fue 0,4310, y la diferenciación genética (Gst de 0,5747. La diversidad genética entre los grupos (57,5 % fue mayor que la detectada dentro de los grupos (42,5 %. Las distancias genéticas entre grupos se calcularon en un rango de 0.1566 a 1,6205. Un alto nivel de heterocigosidad fue observado.

  2. Experiencias y aprendizajes del desarrollo de variedades de frijol de manera participativa en el norte de Nicaragua

    Directory of Open Access Journals (Sweden)

    Conny Almekinders

    2006-01-01

    Full Text Available Esta publicación describe un proyecto piloto de fitomejoramiento en el Norte de Nicaragua. Semillas de frijol de la generación F3 con base en tres cruces triples, hechos en CIAT, Colombia, fueron entregadas a cinco agricultores de dos localidades. Por medio de selección dentro y entre familias, cada agricultor desarrolló una línea avanzada de frijol con resistencia al Mosaico Dorado adaptado a sus condiciones de producción y preferencias. La comparación y validación de las líneas avanzadas con una variedad testigo, resultó en la identificación de dos materiales que se comportaron mejor que el testigo en el área, considerando las evaluaciones de rendimiento, valor comercial (color de grano y características culinarias. Estas líneas promisorias fueron bautizadas y lanzadas en octubre 2004. El proceso de participación es descrito considerando un número de factores incluyendo el proceso en el transcurso de tiempo. Los resultados de este proyecto piloto prueban que es posible desarrollar variedades superiores en campos de agricultores y con la participación de ellos. El desafío del escalamiento e institucionalización presentan preguntas que solamente se puede contestar con experiencias reales

  3. Producción de genotipos de frijol negro en condiciones de humedad residual y sequía terminal

    Directory of Open Access Journals (Sweden)

    Oscar Hugo Tosquy Valle

    2017-01-01

    Full Text Available En Veracruz y Chiapas, el cultivo de frijol del ciclo otoño-invierno, sufre periodos de sequía durante la etapa reproductiva, lo que causa bajos rendimientos. El objetivo de esta investigación fue determinar el rendimiento de genotipos de frijol, evaluados bajo condiciones de humedad residual y de sequía terminal durante el ciclo otoño-invierno de 2013-2014, en las localidades de La Candelaria, Medellín, Veracruz, Campo Experimental Ixtacuaco (CEIXTA, municipio de Martínez de la Torre, Veracruz, y Campo Experimental Centro de Chiapas (CECECH, municipio de Ocozocoautla, Chiapas. Se evaluaron 11 líneas avanzadas de frijol, comparadas con las variedades Negro Tacaná y Negro Jamapa, en diseño experimental bloques al azar con tres repeticiones. Las variables de respuesta fueron: días a floración media, días a madurez fisiológica, peso de 100 semillas y rendimiento de grano. En la Candelaria y el CECECH, ambas con ocurrencia de sequía terminal, se obtuvieron valores significativamente inferiores en las cuatro variables de respuesta, con respecto a los obtenidos en el CEIXTA, sin estrés por sequía. Las líneas SEN-56, SCN-2, SEN-70 y NCB-229 fueron las más precoces en alcanzar su floración media en las tres localidades de prueba, la cual en promedio ocurrió antes de 35 días después de la siembra. Las tres primeras líneas también mostraron menor tiempo a la madurez fisiológica, en promedio antes de los 71 días. La línea SCN-2 obtuvo en promedio el mayor peso de 100 semillas (27 g, el cual fue significativamente superior a los obtenidos por el resto de los genotipos, que registraron pesos menores a 25 g. Así mismo SEN-70, NGO-17-99 y Jamapa Plus, junto con otras cuatro líneas obtuvieron un rendimiento promedio mayor a 1000 kg ha-1, significativamente superior al de las variedades Negro Jamapa y Negro Tacaná. SEN-70 fue la de mayor rendimiento con y sin sequía terminal.

  4. Actividad biológica de la raíz de senecio salignus contra zabrotes subfasciatus en frijol almacenado

    OpenAIRE

    Ernesto López Pérez; Cesáreo Rodríguez Hernández; Laura D. Ortega Arenas; Ramón Garza García

    2007-01-01

    El brúquido Zabrotes subfasciatus es la principal plaga del frijol almacenado en zonas cálidas, y puede ocasionar daño total si no se controla. Se realizó una serie de experimentos en confinamiento y libre elección en condiciones controladas para conocer la actividad biológica del polvo de la raíz de chilca Senecio salignus en Z. subfasciatus, utilizando un diseño completamente al azar. El polvo al 0.01, 0.05, 0.07, 0.1, 0.25, 0.5 y 1% o con tamaños 0.25, 0.25-0.99, 1-2, y 4 mm se mezcló con ...

  5. SELECCIÓN DE GENOTIPOS DE FRIJOL COMÚN POR SU RESISTENCIA AL TIZÓN BACTERIANO

    Directory of Open Access Journals (Sweden)

    Odile Rodr\\u00EDguez-Miranda

    2010-01-01

    Full Text Available En noviembre del 2007, se evaluó la reacción de genotipos de frijol común al tizón común producido por el aislamiento EAP 9506 de Xcp de Honduras y se identificaron nuevas líneas y variedades tolerantes a este patógeno. El estudio se desarrolló en el invernadero del área experimental del Programa de Investigaciones en Frijol (PIF, Escuela Agrícola Panamericana, Zamorano, Honduras. Se sembraron dos experimentos: 1 37 genotipos procedentes de Cuba, 2 27 genotipos procedentes del PIF de Honduras. Las plantas fueron inoculadas con el método de punción múltiple en la etapa V3. La severidad del daño se evaluó a los 20 días después de la inoculación, utilizando una escala visual de 1 a 9 (1=sin síntomas; 9= síntomas severos. El genotipo que mostró reacción de resistencia a Xcp en el experimento 1 fue: XAN 159 ; y en el experimento 2: VAX 3, VAX 5, VAX 6, XAN 309 y XAN 310, y líneas procedentes de la cruza Tío Canela 75/VAX6, destacándose X0104-45-3-5-5, X104-45-5-1-4 y X104-45-5-5-3, con los valores de resistencia más sobresalientes.

  6. Mejoramiento genético para tolerancia a altas temperaturas y resistencia a mosaico dorado en frijol común

    Directory of Open Access Journals (Sweden)

    Juan Carlos Rosas

    2000-01-01

    Full Text Available Mejoramiento genético para tolerancia a altas temperaturas y resistencia a mosaico dorado en frijol común. Entre 1994-95 se identificaron fuentes de frijol tolerantes a altas temperaturas mediante la evaluación de germoplasma y líneas mejoradas en Choluteca y Nacaome (≤ 50 msnm, en la región Sur de Honduras. La tolerancia al calor de los mejores genotipos fue confirmada en Geneva, Nueva York, EE.UU., bajo condiciones de invernadero con temperatura controlada (35/27 oC día/noche. Posteriormente, se realizaron cruzamientos para desarrollar líneas de grano rojo-pequeño tolerantes al calor y resistentes al VMDF y a otros factores limitantes. Las poblaciones segregantes F2-F5 fueron evaluadas por caracteres múltiples incluyendo VMDF, bacteriosis, baja fertilidad, roya, valor agronómico y valor comercial del grano. En 1998, 217 líneas F6 y F7 fueron evaluadas en el distrito de riego Lempa-Acahuapa (20 msnm, región Pacífica de El Salvador, por tolerancia a altas temperaturas y al VMDF. Estas mismas líneas se evaluaron en la localidad de Liberia, en la región Noroeste de Costa Rica, por tolerancia a altas temperaturas; y en El Zamorano, Honduras por resistencia a bacteriosis, mancha angular y roya. Se logró identificar un grupo de líneas que superaron a los testigos comerciales bajo condiciones de altas temperaturas en El Salvador y Costa Rica. Algunas de las líneas seleccionadas presentaron alta resistencia al VMDF en El Salvador, resistencia moderada a mustia en Costa Rica, y excelente comportamiento agronómico y valor comercial del grano

  7. Efecto de la compactación del suelo en la producción de frijol

    Directory of Open Access Journals (Sweden)

    Alfonso Claro A.

    2000-01-01

    Full Text Available En un suelo Ferralítico Rojo (Oxisol representativo de La Llanura Roja del sur de La Habana en vías de degradación por compactación, ubicado en la Estación Experimental de Suelos La Renee, se llevó a cabo en el periodo 1993- 1995, un ensayo de simulación de diferentes grados de compactación, obtenidos por medio de pases sucesivos de grada mediana . El objetivo fue definir la relación entre la baja fertilidad que presentan estos suelos y la compactación antrópica. El cultivo indicador fue el frijol, en relación con maíz. La evaluación de la compactación se efectuó mediante perfiles cartográficos, con los cuales se observó y midió: la penetrometría, el estado interno de la estructura y la colonización radical. Los resultados indicaron una relación directa entre la degradación por compactación y la disminución de la porosidad estructural. El espesor y valor de la capa compacta formada aumentó en dependencia del número de pases de grada. De forma similar la colonización radical se vio restringida a la capa suelta superficial, siendo menos favorecida en la variante de 20 pases de grada, donde su profundidad resultó menor. Los rendimientos de frijol y maíz disminuyeron presentando una relación estrecha con la compactación

  8. Obtención de un híbrido de frijol arbustivo para una cosecha mecanizada

    Directory of Open Access Journals (Sweden)

    Carlos Muñoz Ruiz

    2012-08-01

    Full Text Available En el estudio se evaluaron tres variedades de frijol negro (Brunca, Guaymí y Talamanca, obtenidas en la estación experimental Fabio Baudrit de la Universidad de Costa Rica, y una variedad (Negro Chapingo, obtenida en la Universidad Autónoma de Chapingo, México. Estas variedades no presentaron las características genéticas deseadas para ser utilizadas como donadores y aceptores de polen para obtener un híbrido arbustivo y de floración determinada para su cosecha en forma mecánica en la zona norte del país. Sin embargo, se lograron obtener cinco híbridos F1 entre los cruces realizados, ellos son: BCh, ChT, TCh, BT y TB. Todos ellos presentaron en la F2 características genéticas de porte semiarbustivo (TII y TIII y de floración indeterminada, no compatibles para la obtención de un híbrido adecuado para la cosecha de frijol mecanizada. En este proyecto de investigación se utilizó el método de polinización dirigida o artificial y el cruce simple recíproco entre las variedades participantes y se hicieron 341 polinizaciones entre sí, respectivamente.

  9. Validación de genotipos de frijol negro en la zona central del Estado de Veracruz, México

    Directory of Open Access Journals (Sweden)

    Francisco Javier Ugalde Acosta

    2005-01-01

    Full Text Available Validación de genotipos de frijol negro en la zona central del estado de Veracruz, México. El objetivo del presentetrabajo, fue validar genotipos de frijol con el paquete tecnológico del INIFAP, en parcelas de productores. Durante los ciclosde humedad residual de 1998 a 2001 se establecieron nueveparcelas de validación. Los genotipos utilizados fueron: las lí-neas II-307 y DOR-500 y las variedades Negro Huasteco 81,Negro Cotaxtla 91 y Negro INIFAPy como testigo del productor la variedad Jamapa. En las parcelas se realizaron las laboresde manejo agronómico recomendadas por el INIFAPpara esazona de producción. Cada parcela ocupó una superficie total de3000 m2. Se realizaron análisis de varianza individuales por localidad, considerando como repetición los rendimientos obtenidos por cada genotipo (500 m2 por ciclo de evaluación; también se efectuaron análisis combinado con prueba deseparación de medias (DMS 5% y un análisis económico mediante la Tasa de Retorno Marginal. Los resultados indicaronque las variedades y líneas mejoradas fueron superiores en másdel 100% en rendimiento al testigo. El análisis económicomostró que el mayor beneficio económico se obtuvo al sembrarlas variedades Negro INIFAPy Negro Cotaxtla 91, con utilidades superiores a los US $ 286/ha y una relación beneficio/costo de 1,52 y 1,51, respectivamente.

  10. Interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol

    Directory of Open Access Journals (Sweden)

    Marieta Marín Bruzos

    2013-01-01

    Full Text Available En el estudio se evaluaron, mediante análisis de los parámetros fenológicos de las plantas, la interacción de Tsukamurella paurometabola C-924 con Rhizobium leguminosarum biovar phaseoli CFH en el cultivo de frijol. Se emplearon cuatro tratamientos: plantas sin inocular (control, inoculadas con T. paurometabola C-924, inoculadas con R. leguminosarum biovar phaseoli CFH e inoculadas con ambas cepas. Se observaron diferencias significativas (P < 0.01 en los porcentajes de germinación de las plantas tratadas con microorganismos de forma independiente o conjunta con respecto al control sin inocular. Se determinó que la inoculación de T. paurometabola C-924 afectó el proceso de nodulación de R. leguminosarum biovar phaseoli CFH. Sin embargo, esto no incidió de manera significativa en la altura de las plantas ni en el diámetro del tallo, ya que no se encontraron diferencias entre los tratamientos para estos parámetros. Para el número de hojas, los mejores resultados se obtuvieron con la aplicación de T. paurometabola C-924. Se concluyó que la interacción de T. paurometabola C-924 con R. leguminosarum biovar phaseoli CFH en el frijol estimuló significativamente la germinación de las semillas y el número de hojas de las plantas con respecto al control sin inocular. Aunque la aplicación de T. paurometabola C-924 no favoreció la nodulación de R. leguminosarum biovar phaseoli CFH, esto no afectó las características fenológicas del cultivo.

  11. Comparación de las estructuras morfológicas en raíz e hipocótilo en frijol Comparison of morphological structures in bean root and hypocotyl

    Directory of Open Access Journals (Sweden)

    Edwin Javier Barrios-Gómez

    2012-07-01

    Full Text Available En el año 2006 se realizó un experimento, con el objetivo de determinar las diferencias en desarrollo de variedades contrastantes de frijol (Phaseolus vulgaris L. en su raíz y parte aérea de la plántula. Como se sabe los cultivares tolerantes a sequía tienen vasos de xilema de la raíz e hipocótilo de menor diámetro que los cultivares susceptibles a sequía, se utilizaron cinco materiales de frijol contrastantes, de habito indeterminado tipo III, de los cuales se cosecharon plántulas a los 7,11,15,19 y 23 días después de la siembra (dds; además, en la última cosecha de plántulas (23 dds, se tomaron ejemplares de raíz de aproximadamente 1 cm de longitud. Los cortes en la raíz principal se hicieron en tres posiciones; en la región nodal a 1 cm debajo del eje hipocótilo-raíz, en la parte media y a 1 cm del ápice de cada variedad. El cultivar tolerante a sequía (FM Corregidora tuvo raíces y vasos de xilema de menor diámetro que el cultivar susceptible a sequía (FM RMC y el criollo de bajo rendimiento (Michoacán 128. FM Corregidora mostró mayor longitud y peso seco total de raíces que FM RMC y Michoacán 128. Las raíces de FM Corregidora crecen a mayor velocidad que los demás cultivares, confiriéndole un mayor desarrollo en etapa de plántula. El diámetro del hipocótilo y la oquedad central e s mayor en FM Corregidora y los cultivares de alto rendimiento (FJ Marcela y FM Bajío que en el cultivar susceptible a sequía y el criollo. FM Corregidora, FJ Marcela, FM Bajío y FM RMC tienen corteza más gruesa y mayor número de vasos de xilema que Michoacán 128. El material tolerante a sequía (FM Corregidora mostró raíces y vasos de xilema de menor diámetro que el cultivar susceptible a sequía (FM RMC y la variedad criolla de bajo rendimiento (Michoacán 128.In 2006 an experiment was conducted with the aim to determining the differences in development of contrasting varieties of bean (Phaseolus vulgaris L. root and

  12. Negro Comapa, nueva variedad de frijol para el estado de Veracruz Negro Comapa, a new bean variety for the State of Veracruz

    Directory of Open Access Journals (Sweden)

    Ernesto López Salinas

    2010-12-01

    Full Text Available El programa de frijol del Campo Experimental Cotaxtla, perteneciente al Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP, ubicado en el municipio de Medellín de Bravo, Veracruz; durante varios años se han realizado trabajos de investigación en mejoramiento genético orientados a la generación de nuevas variedades de frijol negro, opaco y pequeño. Actualmente, Chiapas, Nayarit, Oaxaca y Veracruz son las principales entidades productoras de frijol Negro Comapa, en las cuales durante 2008 se sembraron 256 099 hectáreas, principalmente en otoño-invierno con humedad residual, que corresponde a 15.7% de la superficie sembrada en el país. En Veracruz en ese año se sembraron 42 593 hectáreas de frijol, cuyo rendimiento promedio fue de 730 kilogramos por hectárea.During several years in the bean program at Experimental Field Cotaxtla belonging to National Research Forestry, Agriculture and Livestock Institute (INIFAP, located in the municipality of Medellín de Bravo, Veracruz, research works have been carried out in genetic improvement with the aim of generation of new varieties of small opaque black bean. Currently, Chiapas, Nayarit, Oaxaca and Veracruz are the main producers states of this commercial grain type, in which 256 099 hectares were sowed during 2008, mainly in autumn-winter with residual humidity that corresponds to 15.7% of sowed surface in the country. In Veracruz in that year 42 593 hectares of bean were sowed whose average yield was of 730 kilograms per hectare.

  13. Uso de un vivero móvil para obtener patrones de virulencia de la roya del frijol común

    Directory of Open Access Journals (Sweden)

    James Steadman

    2002-01-01

    Full Text Available Después de identificar nuevas fuentes de resistencia a la roya, causada por Uromyces appendiculatus, es necesario saber si las variedades con nuevos genes conservarán su resistencia cuando sean sembradas en diferentes regiones de producción. Para ello, se pueden utilizar viveros móviles con líneas/variedades de frijol con diferentes genes de resistencia para obtener los patrones de virulencia del patógeno de la roya. El vivero contiene plantas de frijol con seis días después de la germinación. El vivero se coloca por dos a tres horas en un campo de frijol infectado con la roya, y luego se exponen a las plantas bajo neblina por un periodo de 15 horas dentro de un ambiente controlado, para un un período de incubación de ocho a diez días en una casa de malla. En las hojas primarias, se efectúan las lecturas del tamaño de la uredinia (pústulas. Se utilizan las reacciones para evaluar los patrones de virulencia y para predecir estrategias de uso de genes de resistencia. El vivero móvil economiza tiempo y dinero (10-12 días comparado con dos a cuatro meses para identificar patotipos de roya con inoculaciones en el invernadero y permite el conocimiento de los resultados durante el ciclo del cultivo del frijol. El concepto del vivero móvil podría ser especialmente útili en paí- ses donde hay una escasez de invernaderos y puede ser utilizado con otros sistemas de cultivos-patógenos.

  14. AKTIVITAS ENZIM FITASE PADA PERKEMBANGAN KACANG HIJAU (Phaseolus radiatus L

    Directory of Open Access Journals (Sweden)

    Woro Riyadina

    2012-09-01

    Full Text Available This is a study on the phytase enzyme activities on germination of Mung Beans (Phaseolus radiatus L. The activities and stability of phytase enzyme were observed under influence of various incubation temperature (27°C, 37°C and 55"C, and incubation time (1 hour, 2 hours and 3 hours of the Mung Beans (Phaseolus radiatus L in germinating for 1 to 5 days. The results showed that activities of phytase enzyme at the same temperature and incubation time are the same in Mung Beans seed germinating for 1 to 5 days. Phytase enzyme is one of the termostabile enzymes with optimal activities at high temperature.

  15. Insects diversity in lima bean (Phaseolus lunatus

    Directory of Open Access Journals (Sweden)

    WIWIN SETIAWATI

    2005-10-01

    Full Text Available Lima bean (Phaseolus lunatus is a vegetable which usually made as a home yard plant for Indonesian people to fulfill their daily needs. This plant has not been produced in the large number by the farmer. So it is hard to find in the market. Lima bean is light by many kind of insect. Inventory, identification and the study of insect taxon to this plant is being done to collect some information about the insect who life in the plant. The research was done in Balitsa experiment garden in the district of Lembang in Bandung regency on November 2003-February 2004, the experiment start at 4 weeks age, at the height of 1260 m over the sea level. The observation was made systematically by absolute method (D-vac macine and relative method (sweeping net. The research so that there were 26 species of phytofagous insect, 9 species of predator insect, 6 species of parasitoid insect, 4 species of pollinator and 14 species of scavenger insect. According to the research the highest species number was got in the 8th week (3rd sampling, which had 27 variety of species, so the highest diversity was also got in this with 2,113 point. Aphididae and Cicadellidae was the most insect found in roay plant. The research also had high number of species insect so the diversity of insect and evenness become high. A community will have the high stability if it is a long with the high diversity. High evenness in community that has low species dominance and high species number of insect so the high of species richness.

  16. Aplicación de metodologías participativas para el mejoramiento genético de frijol en Honduras

    Directory of Open Access Journals (Sweden)

    Juan Carlos Rosas

    2001-01-01

    Full Text Available Aplicación de metodologías participativas para el mejoramiento genético de frijol en Honduras. Aunque se reconoce que los enfoques tradicionales de fitomejoramiento han sido efectivos en incrementar los rendimientos y solucionar problemas de plagas que limitan la producción de cultivos, se argumenta que los niveles de adopción, y los impactos esperados, han sido limitados. Bajo este contexto, se incluyen a los pequeños productores de cultivos básicos, que no poseen los recursos para modificar su ambiente productivo para poder obtener las respuestas en rendimiento de variedades mejoradas de mayor potencial. Sin embargo, en el caso de frijol en Centro América, las variedades mejoradas generadas de manera convencional han sido valiosas en reducir la diseminación de enfermedades que hubieran causado una reducción significativa del rendimiento, pe. el mosaico dorado amarillo. El fitomejoramiento participativo (FP, que involucra una participación activa de los agricultores en el desarrollo de variedades con adaptación específica, se presenta como una alternativa para incrementar el acceso y la adopción de variedades mejoradas para pequeños productores con limitado acceso a las tecnologías y/o bajo uso de insumos. En Centro América, ya existen algunas iniciativas de FP en los cultivos de frijol y maíz; a raíz de estas iniciativas, el interés y las expectativas de los agricultores, organizaciones y donantes ha crecido recientemente. La presente publicación, trata de dar algunas pautas sobre la aplicación de este enfoque de FP en el mejoramiento genético del frijol, con base a la experiencia del Programa de Investigaciones en Frijol de Zamorano, a través de los dos primeros años de actividades de un proyecto conducido con la colaboración de técnicos de una organización no gubernamental y agricultores de comunidades de Yorito y el Lago Yojoa, en Honduras

  17. Evaluation of common bean (Phaseolus vulgaris) response to charcoal rot

    Science.gov (United States)

    Charcoal rot in common beans (Phaseolus vulgaris L.), caused by Macrophomina phaseolina (Tassi) Gold. (Mph), is an endemic disease in the prevailing hot and dry conditions in southern Puerto Rico. This study evaluated the 120 bean genotypes that compose the BASE 120 panel under screenhouse conditio...

  18. CRITICAL PERIOD OF MUNGBEAN (PHASEOLUS RADIATUS L. TO WEED COMPETITION

    Directory of Open Access Journals (Sweden)

    I.H. UTOMO*

    1989-01-01

    Full Text Available A field experiment was conducted to study the critical period of weed control on the crop of mungbean (Phaseolus radiatus L.. The studies were done in the field of BIOTROP Experimental Station with the natural existing weed population. It was found that the critical period of mungbean to weed competition was from 3-6 weeks after planting.

  19. CRITICAL PERIOD OF MUNGBEAN (PHASEOLUS RADIATUS L.) TO WEED COMPETITION

    OpenAIRE

    I.H. UTOMO*

    1989-01-01

    A field experiment was conducted to study the critical period of weed control on the crop of mungbean (Phaseolus radiatus L.). The studies were done in the field of BIOTROP Experimental Station with the natural existing weed population. It was found that the critical period of mungbean to weed competition was from 3-6 weeks after planting.

  20. Oriënterend onderzoek over de reuzen- en dwergplanten in F1 en volgende generaties van Phaseolus vulgaris L. x Phaseolus multiflorus Lam.

    NARCIS (Netherlands)

    Geerts, Sipke Johannes

    1949-01-01

    A cross is studied between the selffertilising bush bean (Phaseolus vulgaris L.) "Zeeuwse Bruine Boon" and crossfertilising runner bean (Phaseolus multiflorus Lam.) "stam" (a scarlet flowering stockrunner-bean) or some other (climbing) runners. With the bushbean as mother this cross easily results i

  1. Oriënterend onderzoek over de reuzen- en dwergplanten in F1 en volgende generaties van Phaseolus vulgaris L. x Phaseolus multiflorus Lam.

    NARCIS (Netherlands)

    Geerts, Sipke Johannes

    1949-01-01

    A cross is studied between the selffertilising bush bean (Phaseolus vulgaris L.) "Zeeuwse Bruine Boon" and crossfertilising runner bean (Phaseolus multiflorus Lam.) "stam" (a scarlet flowering stockrunner-bean) or some other (climbing) runners. With the bushbean as mother this cross easily results i

  2. Asbestos in Colorado Schools.

    Science.gov (United States)

    Baldwin, Cynthia A.

    This study determined, by means of a random sample, how many of Colorado's public schools have asbestos materials and estimated the potential risk of exposure presented by these materials. Forty-one schools were surveyed. Bulk samples of possible asbestos materials were collected and analyzed using the K-squared Asbestos Screening Test to…

  3. Colorado's Singular "No"

    Science.gov (United States)

    Wiedeman, Reeves

    2008-01-01

    Supporters of affirmative action may have finally found a way to defeat state ballot measures that would ban such programs: Latch onto an inspirational presidential candidate with piles of cash and an unprecedented voter-turnout machine. Those activists won a narrow victory in Colorado this month, when 50.7 percent of voters made the state the…

  4. Asbestos in Colorado Schools.

    Science.gov (United States)

    Baldwin, Cynthia A.

    This study determined, by means of a random sample, how many of Colorado's public schools have asbestos materials and estimated the potential risk of exposure presented by these materials. Forty-one schools were surveyed. Bulk samples of possible asbestos materials were collected and analyzed using the K-squared Asbestos Screening Test to…

  5. Game Birds of Colorado.

    Science.gov (United States)

    Colorado State Div. of Wildlife, Denver.

    This booklet is intended to familiarize the reader with game birds typical of Colorado. Discussions in English and Spanish are presented. Discussions cover the management of game birds, individual game bird species, and endangered species of birds related to game birds. (RE)

  6. Flor de Mayo Eugenia, nueva variedad de frijol para riego y temporal en el centro de México Flor de Mayo Eugenia, new bean cultivar for irrigated and rainfall conditions in Central Mexico

    OpenAIRE

    Jorge Alberto Acosta Gallegos; Yanet Jiménez Hernández; Bertha María Sánchez García; Francisco M. Mendoza Hernández; María Guadalupe Herrera Hernández; Rafael A. Salinas Pérez; Mario González Chavira

    2010-01-01

    Dentro del frijol tipo Flor de Mayo, el subtipo 'media oreja' esta cobrando importancia en el norte-centro de México, éste es de tamaño mediano, forma alargada y color rosa atractivo. Se describe una nueva variedad de este tipo de frijol, Flor de Mayo Eugenia (FME). FME se derivó de una cruza interracial entre Flor de Mayo Anita (FMA) (raza Jalisco) y Rayado Rojo (raza Nueva Granada). El rendimiento promedio de FME en temporal varia de 0.8 a 2.0 t ha-1 y bajo riego el máximo rendimiento ha si...

  7. Resistencia a sequía terminal en frijol negro tropical Comportamiento de variedades de maíz normal y con alta calidad de proteína para la región Golfo de México

    OpenAIRE

    Mark Frahm; Juan Carlos Rosas; Netzahualcoyotl Mayek; Ernesto L\\u00F3pez; Jorge A. Acosta; Kelly, James D.

    2003-01-01

    Con el objetivo de identificar cultivares de frijol negro tropical con resistencia a sequía y adaptación a la región Centroamericana, se diseñó un proyecto piloto colaborativo entre los programas de frijol de Zamorano, Honduras; Veracruz, México y la Universidad Estatal de Michigan (MSU); EE.UU, en el año 2002. Se desarrollaron dos poblaciones de líneas endog ámicas recombinantes (RILs) derivadas de cruces entre la lí- nea resistente a sequía B98311 de MSU con TLP 19 y VAX 5, dos líneas mejor...

  8. Caracterización de los productores, adopción e impacto económico del uso de la variedad de frijol "Pinto Saltillo" en el norte centro de México

    OpenAIRE

    Mario René Ávila Marioni; José de Jesús Espinoza Arellano; Horacio González Ramírez; Rigoberto Rosales Serna; Arnulfo Pajarito Ravelero; Román Zandate Hernández

    2011-01-01

    En la región Norte-Centro de México, principal zona productora de frijol en el país se realizó un estudio durante el período 2008-2009, cuyos objetivos fueron caracterizar a los productores, determinar el grado de adopción y evaluar el impacto del uso de la variedad de frijol Pinto Saltillo, generada por el INIFAP. El procedimiento metodológico se basó en una entrevista formal a una muestra de agricultores, los cuales fueron seleccionados al azar de una población de 45,000 productores registr...

  9. Libraries in Colorado: MedlinePlus

    Science.gov (United States)

    ... Library → Libraries in Colorado URL of this page: https://medlineplus.gov/libraries/colorado.html Libraries in Colorado ... Room 2106C Aurora, CO 80045 303-724-2111 http://hslibrary.ucdenver.edu/ Denver National Jewish Health Library ...

  10. Silverton folio, Colorado

    Science.gov (United States)

    Cross, Whitman; Howe, Ernest; Ransome, F. L.

    1905-01-01

    The term San Juan region, or simply "the San Juan," used with variable meaning by early explorers, and naturally with indefinite limitation during the period of settlement, is now quite generally applied to a large tract of mountainous country in southwestern Colorado, together with an undefined zone of lower country bordering it on the north, west, and south.  The Continental Divide traverses this area in a great bow.  The principal part of the district is a deeply scored volcanic plateau, more than 3000 square miles in extent, drained on the north by the tributaties of the Gunnison River, on the west by those of the Dolores and San Miguel rivers, on the south by numerous branches of the San Juan, and on the east by the Rio Grande.  ALl but the latter drainage finds its way to the Gulf of California through the Colorado River.

  11. PRODUCCIÓN ARTESANAL DE SEMILLA DE FRIJOL CON PARTICIPACIÓN MUNICIPAL EN VERACRUZ, MÉXICO

    Directory of Open Access Journals (Sweden)

    Francisco Javier Ugalde-Acosta

    2014-01-01

    Full Text Available El objetivo de este trabajo fue evaluar la producción artesanal de semilla de frijol municipal (PASF-Municipal. La estrategia se implementó de 1998-2010, en veintitrés municipios de las regiones Centro- sur y de las Altas Montañas del estado de Veracruz, México. El estudio constó de dos fases: una de establecimiento de parcelas de validación de variedades para seleccionar las de mayor productividad; la segunda fase fue la de instalación de parcelas de producción de semilla de las variedades mejoradas seleccionadas. Durante la validación en la región Centro-sur, las mejoradas superaron en 78,7% al testigo Negro Michigan (743 kg/ha. En las Altas Montañas, los cultivares mejorados registraron un rendimiento de 1219 kg/ ha, mientras que Negro Michigan produjo 751 kg/ha. En la producción de semilla, se obtuvieron 28 300 kilogramos, para la siembra de 628 hectáreas en beneficio de 1545 productores. El incremento promedio obtenido por los productores con semilla PASF-Municipal de variedades mejoradas fue de 109,4% en relación a la obtenida con el grano utilizado como semilla de la variedad del productor.

  12. Respose of Phaseolus vulgaris L. cultivars to drought stress

    Directory of Open Access Journals (Sweden)

    Amalia Domínguez

    2014-01-01

    Full Text Available Tolerance to drought is a desired cultivars of Phaseolus vulgaris L. The present study aimed to determine the response of 22 cultivars of common bean during the early stages of vegetative development character. The plants were subjected to irrigation at 70% or 20% of field capacity (FC for seven days and the indicators were measured relative water content, stomatal opening, stomatal index, proline content and total phenols in leaves. The data obtained were processed using a principal component analysis and the variables studied were represented by a bivariate graph (biplot. It was possible to group the cultivars based on their response in tolerant, moderately tolerant and susceptible to water stress condition induced by irrigation at 20% FC. Stomatal opening and relative water content were recommended to be used as criteria for selecting cultivars tolerant to water stress indicators bean. Key words: PCA, Phaseolus vulgaris L., proline, water stress

  13. Standardization of phaseolus vulgaris valves by macro- and microscopic characteristics

    Directory of Open Access Journals (Sweden)

    Анна Ігорівна Крюкова

    2016-03-01

    Full Text Available During the last years on the Ukrainian pharmaceutical market a tendency to increase the range of the national herbal remedies is observed. Native flora herbs, having a sufficient resource base, deserve special attention and are characterized by a number of economic benefits for manufacturers. The implementation of herbal remedies in medical practice is accompanied by the necessity to improve the quality control of herbal material and to develop normative documents. Macro- and microscopy analysis becomes important at standardization of herbal material especially cut and powdered.Aim. The aim of our work was to carry out macro- and microscopy research of Phaseolus vulgaris and to determine its diagnostic morphologic and anatomic features.Methods. The conventional research methods in pharmacognosy have been used; photomicrography has been taken by using microscope MC-10 and Samsung PL 50 camera.Results. As a result of research, the microscopic diagnostic characteristics for Phaseolus vulgaris valves have been proposed, e.g.: the valves are elongated, straight, navicular or grooved, twisted to some extent; the external surface is smooth or slightly undulating, mat, pale yellow or yellow; the internal surface is shiny, white or yellowish-white. Sometimes carpophore (stalk may occur. The microscopic diagnostic features have been determined and offered, e.g.: cells of outer epidermis are pentagonal and hexagonal, with plicated cuticle; stomata are surrounded by 3–4 subsidiary narrow cells, additionally surrounded by 5–6 narrow cells; cover trichomes and glandular trichomes are elongated, pointed and unbranched; xylem vessels and tracheids; non-bundled structure stalk fragments with pericyclic sclerenchyma and secretory cells in the phloem.Conclusion. Research on determination of the macro- and microscopic characteristics of Phaseolus vulgaris valves has been carried out. The diagnostic morphological and anatomic elements in herbal material have

  14. Estadísticos vitales de Bemisia tabaci biotipo B en frijol e interacción con el parasitoide Amitus fuscipennis: Life history parameters and absence of hostparasitoid interaction with Amitus fuscipennis Bemisia tabaci biotype B in bean

    Directory of Open Access Journals (Sweden)

    María del Rosario Manzano Martínez

    2009-10-01

    Full Text Available Se determinaron los estadísticos vitales de la mosca blanca Bemisia tabaci biotipo B en condiciones de laboratorio (26 °C ± 2 °C y 80 ± 5 % de HR en fríjol cultivar ICA-Pijao. Los valores promedios fueron: longevidad 34 ± 1.64 días, fecundidad de 211.4 ± 15.1 huevos/hembra y tasa de oviposición de 6.1 ± 0.28 huevos/día. La tasa neta reproductiva fue de 137.8. El tiempo generacional de 43.8 días y la tasa intrínseca de crecimiento poblacional (r m de 0.125. Estos valores son coincidentes con los reportados en la literatura e indicaron la utilidad del frijol (Phaseolus vulgaris L ICA-Pijao para la multiplicación de B. tabaci. No se logró la reproducción de Amitus fuscipennis en B. tabaci. Estudios de comportamiento mostraron que el parasitoide permaneció solo 617.3 seg en promedio en foliolos infestados con la mosca blanca, la mayor parte del tiempo lo invirtió en caminar (46.4%, limpiarse el cuerpo (35.6% y permanecer quieta (16.7%. A. fuscipennis solo tocó una vez accidentalmente una ninfa de B. tabaci, pero no la examinó con las antenas ni la ovipositó. Los resultados indican que A. fuscipennis no es un enemigo natural potencial de B. tabaci biotipo B.Life-history parameters of the whitefly Bemisia tabaci biotipo B were determined at laboratory conditions at 26 °C ± 2 °C y 80 ± 5 % de RH on bean ICA-Pijao. Mean longevity of the whitefly was 34 ± 1,64 days, mean total fecundity was 211,4 ± 15,1 eggs per female with a daily oviposition rate of 6,1 ± 0,28 eggs per day. Net reproduction rate was 137,8. Generation Time was 43,8 days and the intrinsic rate of population increase r m was 0,125. These values are according to previous ones reported and support usefulness of ICA-Pijao bean for B. tabaci rearing. It was not possible to rear Amitus fuscipennis from B. tabaci. Behaviour studies showed that the parasitoid stayed only average 617,3 s on whitefly infested leaflets. It invested most of the time walking (46

  15. ALTERACIONES ULTRAESTRUCTURALES EN LOS CLOROPLASTOS Y NÓDULOS DE LAS RAÍCES DE PLANTAS DE FRIJOL CAUPÍ DESARROLLADAS BAJO CONDICIONES DE ESTRÉS SALINO

    OpenAIRE

    Ernesto Gómez Padilla; Beatriz Ruiz-Diez; Susana Fajardo; Bettina Eichler-Loebermann; Raúl López Sánchez; Mercedes Fernández-Pascual

    2014-01-01

    Se ha evaluado el efecto del estrés salino sobre la ultraestructura de los cloroplastos y los nódulos radicales de plantas de frijol Caupí ( Vigna unguiculata (L.) Walp.), var . IT 86 D-715, mediante Microscopía Electrónica de Transmisión (TEM). Las plantas se sometieron a 150 y 0,02 mM de NaCl, siendo considerado este último como control. La cepa VIBA-1 ( Bradyrhizobium liaoningense ), aislada de suelos salinos del Valle del Cauto (Cuba), fue inoculada en el momento de la siembra. Cuarenta d...

  16. Contenido de osmoprotectores, ácido ascórbico y ascorbato peroxidasa en hojas de frijol sometidas a estrés por sequía

    OpenAIRE

    Teresa Susana Herrera Flores; Joaquín Ortíz Cereceres; Adriana Delgado Alvarado; Jorge Alberto Acosta Galleros

    2014-01-01

    La acumulación de osmoprotectores en plantas que se encuentran en condiciones de estrés por sequía, le permiten contrarrestar el efecto negativo que le provoca dichas condición ambiental. En el presente trabajo se estudió la respuesta bioquímica de plantas de frijol con base en la acumulación de carbohidratos solubles, almidón, ácido ascórbico, así como la actividad de la enzima ascorbato peroxidasa, en las hojas simples y en las dos primeras hojas trifolioladas de la variedad resistente, Pin...

  17. Prácticas de conservación de suelos en sistemas de cultivo maíz-frijol en Cuba

    OpenAIRE

    2000-01-01

    Se realizaron dos ensayos en segmentos de microcuencas en condiciones de producción de la Provincia de Pinar del Río: uno en Campo Hermoso y el otro en La Comunidad La Leña, Cuba. En ambas localidades se rotó el frijol variedad Bat 304 y Tomeguin 93, seguidos de maíz asociado con Mucuna deeringianum y se establecieron bordes de desagüe protegidos con barreras vivas de Leucaena leucocephala y Gliricidia sepium. Las pérdidas de suelos se estimaron en piscinas de sediment...

  18. Uso y difusión del frijol de abono (Mucuna deeringiana) en las laderas del litoral Atlántico de Honduras.

    OpenAIRE

    Buckles, Daniel; Ponce, Ignacio; Sain, Gustavo; Medina, Gilmer

    2016-01-01

    La conservación del suelo, los nutrimentos y el agua son los principales problemas que afrontan los agricultores e investigadores agrícolas en los sistemas de cultivo basados en el maíz en Centroamérica y México. Algunos productores de Honduras han ideado y refinado una tecnología eficaz para el cultivo del maíz en ladera en la que se siembra el frijol de abono (Mucuna deeringiana) en rotación con el maíz de postrera (o temporada seca). Los resultados de la encuesta realizada en el Departamen...

  19. "Azufrasin": nueva variedad de frijol tipo Azufrado para el estado de Sinaloa "Azufrasin": a new yellow seeded dry bean cultivar for the state of Sinaloa

    Directory of Open Access Journals (Sweden)

    Rafael Atanasio Salinas Pérez

    2011-08-01

    Full Text Available En el estado de Sinaloa, México el frijol amarillo del tipo Azufrado-Peruano, raza Nueva Granada, es el que mayor superficie ocupa para satisfacer la demanda interna y para la exportación a EE.UU. Se describe el desarrollo y características agronómicas de la nueva variedad de frijol, 'Azufrasin'; cuya utilización permitirá ampliar el número de variedades disponibles de este frijol en el estado. Esta nueva variedad se desarrolló a partir de la cruza de tres líneas entre ((Azufrado Pimono-78/Canario-72//AZPA-5; durante el desarrollo la selección se basó en la tolerancia a las enfermedades virales (BCMV y BCMNV, moho blanco (Sclerotinia sclerotorium y resistencia a la roya (Uromyces appendiculatus var. appendiculatus. Azufrasin es de hábito determinado tipo I, de porte alto con plantas de 45 cm, 100 días a la madurez fisiológica y el peso de cien semillas varia de 44 a 46 g. Azufrasin muestra adaptación en las áreas productoras de frijol del norte de Sinaloa bajo condiciones de riego, donde ha mostrado alta capacidad de rendimiento y alto grado de tolerancia a enfermedades durante el ciclo de otoño-invierno, superando en rendimiento a la variedad Azufrado Higuera con un promedio de 339 kg ha-1.In the state of Sinaloa, Mexico; the yellow bean of Azufrado-Peruano, Nueva Granada race, which occupies most of the surface area to meet the domestic demand and for exporting to the United States. The development and agronomic characteristics of the new bean variety are described, 'Azufrasin'; whose use will expand the number of available varieties of this bean in the state. This new variety was developed from crosses between three lines ((Azufrado Pimono-78/Canario-72//AZPA-5; during the development, the selection was based on tolerance to viral diseases (BCMV and BCMNV, white mold (Sclerotinia sclerotorium and resistance to rust (Uromyces appendiculatus var. appendiculatus. Azufrasin has a determinate growth habit (Type I, high bearing

  20. Beans (Phaseolus ssp.) as a Model for Understanding Crop Evolution

    Science.gov (United States)

    Bitocchi, Elena; Rau, Domenico; Bellucci, Elisa; Rodriguez, Monica; Murgia, Maria L.; Gioia, Tania; Santo, Debora; Nanni, Laura; Attene, Giovanna; Papa, Roberto

    2017-01-01

    Here, we aim to provide a comprehensive and up-to-date overview of the most significant outcomes in the literature regarding the origin of Phaseolus genus, the geographical distribution of the wild species, the domestication process, and the wide spread out of the centers of origin. Phaseolus can be considered as a unique model for the study of crop evolution, and in particular, for an understanding of the convergent phenotypic evolution that occurred under domestication. The almost unique situation that characterizes the Phaseolus genus is that five of its ∼70 species have been domesticated (i.e., Phaseolus vulgaris, P. coccineus, P. dumosus, P. acutifolius, and P. lunatus), and in addition, for P. vulgaris and P. lunatus, the wild forms are distributed in both Mesoamerica and South America, where at least two independent and isolated episodes of domestication occurred. Thus, at least seven independent domestication events occurred, which provides the possibility to unravel the genetic basis of the domestication process not only among species of the same genus, but also between gene pools within the same species. Along with this, other interesting features makes Phaseolus crops very useful in the study of evolution, including: (i) their recent divergence, and the high level of collinearity and synteny among their genomes; (ii) their different breeding systems and life history traits, from annual and autogamous, to perennial and allogamous; and (iii) their adaptation to different environments, not only in their centers of origin, but also out of the Americas, following their introduction and wide spread through different countries. In particular for P. vulgaris this resulted in the breaking of the spatial isolation of the Mesoamerican and Andean gene pools, which allowed spontaneous hybridization, thus increasing of the possibility of novel genotypes and phenotypes. This knowledge that is associated to the genetic resources that have been conserved ex situ and in

  1. Aluminium-phosphate interactions in the rhizosphere of two bean species: Phaseolus lunatus L. and Phaseolus vulgaris L.

    Science.gov (United States)

    Mimmo, Tanja; Ghizzi, Massimiliano; Cesco, Stefano; Tomasi, Nicola; Pinton, Roberto; Puschenreiter, Markus

    2013-12-01

    Plants differ in their response to high aluminium (Al) concentrations, which typically cause toxicity in plants grown on acidic soils. The response depends on plant species and environmental conditions such as substrate and cultivation system. The present study aimed to assess Al-phosphate (P) dynamics in the rhizosphere of two bean species, Phaseolus vulgaris L. var. Red Kidney and Phaseolus lunatus L., in rhizobox experiments. Root activity of the bean species induced up to a sevenfold increase in exchangeable Al and up to a 30-fold decrease in extractable P. High soluble Al concentrations triggered the release of plant-specific carboxylates, which differed between soil type and plant species. The results suggest that P. vulgaris L. mitigates Al stress by an internal defence mechanism and P. lunatus L. by an external one, both mechanisms involving organic acids. Rhizosphere mechanisms involved in Al detoxification were found to be different for P. vulgaris L. and P. lunatus L., suggesting that these processes are plant species-specific. Phaseolus vulgaris L. accumulates Al in the shoots (internal tolerance mechanism), while P. lunatus L. prevents Al uptake by releasing organic acids (exclusion mechanism) into the growth media. © 2013 Society of Chemical Industry.

  2. 77 FR 15798 - Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs, CO

    Science.gov (United States)

    2012-03-16

    ... National Park Service Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs..., Colorado College, Armstrong Hall, Room 201, 14 E. Cache La Poudre, Colorado Springs, CO 80903, telephone... as the Taylor Museum and the Colorado Springs Fine Arts Center) and the Denver Museum of...

  3. 77 FR 23498 - Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs, CO

    Science.gov (United States)

    2012-04-19

    ... National Park Service Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs..., Colorado College, Armstrong Hall, Room 201, 14 E. Cache La Poudre, Colorado Springs, CO 80903, telephone... Fine Arts Center (formerly known as the Taylor Museum and the Colorado Springs Fine Arts Center)...

  4. Variación patogénica y fuentes de resistencia a Colletotrichum lindemuthianum Sacc & magn Scrib., patógeno de la antracnosis del frijol, en Colombia Variación patogénica y fuentes de resistencia a Colletotrichum lindemuthianum (Sacc & Magn) Scrib., patógeno de la antracnosis del fríjol, en Colombia

    National Research Council Canada - National Science Library

    Cobo S. Felipe; Pastor Corrales Marcial A

    1987-01-01

    ... patogénica de aislamientos colombianos de Colletotrichum lindemuthianum (Sacc& Magn) Scrib. También se evaluó germoplasma de frijol con grano de color comercial con el objetivo de identificar...

  5. Respuesta fotosintética de algunas variedades de maíz, frijol y café

    Directory of Open Access Journals (Sweden)

    Anundo Polanía

    2009-06-01

    Full Text Available Con el objeto de caracterizar fotosintéticamente algunas variedades de maíz, frijol y café, se estudió el comportamiento de la rata de fotosíntesis neta, de la transpiración y de los diferentes tipos de resistencia foliar a ia difusión y fijación de CO2 en la hoja, frente a las variables temperatura, intensidad de luz y concentración de CO2 en el aire. La variedad de maíz ICA H 507 presentó un comportamiento típico de plantas C 4 con altas ratas de fotosíntesis neta, insaturación de luz y baja fotorespiración. El fríjol Dlacol Andino presentó bajas ratas de fotosíntesis, alta fotorespiración y saturación de luz a bajas Intensidades, características propias de plantas C3. Las dos varíedades de café, Típica y Caturra, presentaron bajas ratas de fotosíntesis, valores de fotorespiración cercanos a los del fríjol, comportamiento típico de plantas C3; no lo es su respuesta a la intensidad de luz, ya que se saturaron a intensidades más altas que las presentadas por plantas C3 reconocidas. Estas varíedades presentaron ratas de fotosíntesis mayores que las observadas por otros autores en plantas de café de más edad. Los resultados obtenidos brindan información útil acerca del comportamiento ecoflslológico de

  6. Near Surface Structure of the Frijoles Strand of the San Gregorio Fault, Point Año Nuevo, San Mateo County, California, from Seismic Imaging

    Science.gov (United States)

    Campbell, L.; Catchings, R. D.; Rymer, M. J.; Goldman, M.; Weber, G. E.

    2012-12-01

    The San Gregorio Fault Zone (SGFZ) is one of the major faults of the San Andreas Fault (SAF) system in the San Francisco Bay region of California. The SGFZ is nearly 200 km long, trends subparallel to the SAF, and is located primarily offshore with two exceptions- between Point Año Nuevo and San Gregorio Beach and between Pillar Point and Moss Beach. It has a total width of 2 to 3 km and is comprised of seven known fault strands with Quaternary activity, five of which also demonstrate late Holocene activity. The fault is clearly a potential source of significant earthquakes and has been assigned a maximum likely magnitude of 7.3. To better understand the structure, geometry, and shallow-depth P-wave velocities associated with the SGFZ, we acquired a 585-m-long, high-resolution, combined seismic reflection and refraction profile across the Frijoles strand of the SGFZ at Point Año Nuevo State Park. Both P- and S-wave data were acquired, but here we present only the P-wave data. We used two 60-channel Geometrics RX60 seismographs and 120 40-Hz single-element geophones connected via cable to record Betsy Seisgun seismic sources (shots). Both shots and geophones were approximately co-located and spaced at 5-m intervals along the profile, with the shots offset laterally from the geophones by 1 m. We measured first-arrival refractions from all shots and geophones to develop a seismic refraction tomography velocity model of the upper 70 m. P-wave velocities range from about 600 m/s near the surface to more than 2400 m/s at 70 m depth. We used the refraction tomography image to infer the depth to the top of the groundwater table on the basis of the 1500 m/s velocity contour. The image suggests that the depth, along the profile, to the top of groundwater varies by about 18 m, with greater depth on the west side of the fault. At about 46 m depth, a 60- to 80-m-wide, low-velocity zone, which is consistent with faulting, is observed southwest of the Frijoles strand of the

  7. Colorado State Capitol Geothermal project

    Energy Technology Data Exchange (ETDEWEB)

    Shepherd, Lance [Colorado Department of Personnel and Adminstration, Denver, CO (United States)

    2016-04-29

    Colorado State Capitol Geothermal Project - Final report is redacted due to space constraints. This project was an innovative large-scale ground-source heat pump (GSHP) project at the Colorado State Capitol in Denver, Colorado. The project employed two large wells on the property. One for pulling water from the aquifer, and another for returning the water to the aquifer, after performing the heat exchange. The two wells can work in either direction. Heat extracted/added to the water via a heat exchanger is used to perform space conditioning in the building.

  8. Colorado wetlands initiative : 1997-2000 : Protecting Colorado's wetlands resource

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Colorado Wetlands Initiative is an endeavor to protect wetlands and wetland-dependent wildlife through the use of voluntary, incentive-based mechanisms. It is a...

  9. The Paleobiolinguistics of the Common Bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Cecil H. Brown

    2014-10-01

    Full Text Available Paleobiolinguistics is used to determine when and where the common bean (Phaseolus vulgaris L. developed significance for prehistoric groups of Native America. Dates and locations of proto-languages for which common bean terms reconstruct generally accord with crop-origin and dispersal information from plant genetics and archaeobotany. Paleobiolinguistic and other lines of evidence indicate that human interest in the common bean became significant primarily with the widespread development of a village‐farming way of life in the New World rather than earlier when squash and maize and a few other crops became important.

  10. The Colorado Adoption Project.

    Science.gov (United States)

    Plomin, R; DeFries, J C

    1983-04-01

    This report provides an overview of the Colorado Adoption Project (CAP), a longitudinal, prospective, multivariate adoption study of behavioral development. Examples of the types of analyses that can be conducted using this design are presented. The examples are based on general cognitive-ability data for adoptive, biological, and control parents; assessments of their home environment; and Bayley Mental Development Index scores for 152 adopted children and 120 matched control children tested at both 1 and 2 years of age. The illustrative analyses include matched control children tested at both 1 and 2 years of age. The illustrative analyses include examination of genetic and environmental sources of variance, identification of environmental influence devoid of genetic bias, assessment of genotype-environment interaction and correlation, and analyses of the etiology of change and continuity in development.

  11. Pikes Peak, Colorado

    Science.gov (United States)

    Brunstein, Craig; Quesenberry, Carol; Davis, John; Jackson, Gene; Scott, Glenn R.; D'Erchia, Terry D.; Swibas, Ed; Carter, Lorna; McKinney, Kevin; Cole, Jim

    2006-01-01

    For 200 years, Pikes Peak has been a symbol of America's Western Frontier--a beacon that drew prospectors during the great 1859-60 Gold Rush to the 'Pikes Peak country,' the scenic destination for hundreds of thousands of visitors each year, and an enduring source of pride for cities in the region, the State of Colorado, and the Nation. November 2006 marks the 200th anniversary of the Zebulon M. Pike expedition's first sighting of what has become one of the world's most famous mountains--Pikes Peak. In the decades following that sighting, Pikes Peak became symbolic of America's Western Frontier, embodying the spirit of Native Americans, early explorers, trappers, and traders who traversed the vast uncharted wilderness of the Western Great Plains and the Southern Rocky Mountains. High-quality printed paper copies of this poster are available at no cost from Information Services, U.S. Geological Survey (1-888-ASK-USGS).

  12. Colorados asutati rohelise ehituse toetusprogramm

    Index Scriptorium Estoniae

    2002-01-01

    USA Colorado osariigi rohelised arhitektid ja projekteerijad asutasid koos ehitusfirmadega programmi "Ehita rohelist Coloradot", mille raames pakutakse rohelise maja või korteri ehitamise väljaõpet

  13. Colorados asutati rohelise ehituse toetusprogramm

    Index Scriptorium Estoniae

    2002-01-01

    USA Colorado osariigi rohelised arhitektid ja projekteerijad asutasid koos ehitusfirmadega programmi "Ehita rohelist Coloradot", mille raames pakutakse rohelise maja või korteri ehitamise väljaõpet

  14. Monitoreo de los materiales escritos y de los puestos de información de una campaña educativa para aumentar el consumo de frijoles

    Directory of Open Access Journals (Sweden)

    Marcela Dumani

    2004-01-01

    Full Text Available Monitoreo de los materiales escritos y de los puestos de información de una Campaña educatitva para aumentar el consumo de frijoles. Se presentan los resultados del monitoreo de los principales materiales educativos y de algunas actividades interactivas desarrolladas en la etapa de ejecución de una Campaña educativa para incrementar el consumo de frijoles. El estudio incluye el seguimiento de un recetario y dos panfletos desplegables, así como de los puestos promocionales ubicados en los supermercados. Los materiales y las actividades de la Campaña fueron elaborados y planeados tomando en cuenta los criterios aportados por la población que participó en la etapa diagnóstica de la investigación. Para valorar la contribución a la Campaña del material y las actividades educativas ya mencionadas, se obtuvieron las opiniones de la población hacia la cual iban dirigidos. Esto permitió, determinar su utilidad para campañas o programas educativos similares en el futuro. El recetario “De Todo con Frijoles” constituyó un recurso importante para la Campaña. Este material tuvo una amplia aceptación por parte de las madres. El monitoreo aplicado a los panfletos desplegables permitió confirmar que las mujeres valoran la información que se ofrece en un material educativo impreso y que la forma de ilustrarlo y presentarlo, complementan la información y captan la atención de la población. Las actividades interactivas son importantes en una campaña educativa, ya que permiten el contacto directo con la población, la evacuación de dudas y la generación de información adicional sobre las percepciones que tienen las personas en relación con el alimento que se promueve. El monitoreo de estas actividades posibilitó realizar ajustes sobre la marcha que favorecieron el cumplimiento de los objetivos de la Campaña..

  15. Janasa, nueva variedad de frijol tipo azufrado para el estado de Sinaloa, México Janasa, new variety of Azufrado type beans for the state of Sinaloa, Mexico

    Directory of Open Access Journals (Sweden)

    Rafael Atanasio Salinas Pérez

    2012-04-01

    Full Text Available En Sinaloa el frijol de grano amarillo del tipo Azufrado-Peruano, raza Nueva Granada, particularmente la variedad Azufrado Higuera, ocupa la mayor superficie cultivada con esta leguminosa. En esta nota se describe el desarrollo y características agronómicas de una nueva variedad de este tipo de frijol, "Janasa"; la cual permitirá ampliar el número de variedades disponibles en esta clase comercial. Esta nueva variedad se desarrolló a partir de la cruza de tres líneas entre ((Azufrado Pimono-78 / Canario-72 // AZPA-5. Durante el desarrollo la selección se baso en la tolerancia a las enfermedades virales (BGMV, BCMV y BCMNV y fungosas como son el moho blanco (Sclerotinia sclerotorium y roya (Uromyces appendiculatus var. appendiculatus. La planta de Janasa es de hábito determinado tipo I, de porte alto con 44 cm, 104 días a la madurez fisiológica y su peso de cien semillas varia de 42 a 44 g. Janasa muestra adaptación en las áreas productoras de frijol del norte de Sinaloa bajo condiciones de riego, donde ha mostrado alto potencial de rendimiento y tolerancia a enfermedades durante el ciclo de otoño-invierno, superando en rendimiento a la variedad Azufrado Higuera con un promedio de cuatro años en 322 kg ha-¹.In Sinaloa, the yellow-grained bean of the type Azufrado-Peruvian, Nueva Granada breed, particularly the variety Azufrado Higuera, has the greatest area planted with this legume. In this article, we describe the development and the agronomic characteristics of a new variety of this type of bean, "Janasa", which will help broaden the number of varieties available in this commercial class. This new variety was developed from the breeding of three lines between ((Azufrado Pimono-78 / Canario-72 // AZPA-5. The development of the selection was based on the tolerance to viral (BGMV, BCMV and BCMNV and fungal diseases such as (Sclerotinia sclerotorium and rust (Uromyces appendiculatus var. appendiculatus. The Janasa plant has determined

  16. Análisis proteómico de la resistencia inducida por micorrización al patógeno foliar Sclerotinia sclerotiorum en frijol (Phaseolus vulgaris l.)

    OpenAIRE

    2014-01-01

    En los últimos años se han reportado diversos estudios sobre la resistencia inducida por micorrización al ataque de patógenos, en éstos se han determinado los cambios de expresión de genes y proteínas que ocurren en las raíces de plantas colonizadas por hongos micorrízicos arbusculares. Recientemente, se han iniciado estudios para entender cómo afecta esta interacción a la parte aérea de la planta. En nuestro grupo de investigación se llevó a cabo el estudio del efecto de la micorrizaci...

  17. Elongación del hipocótilo en plantas de frijol (phaseolus vulgaris por tratamiento de embriones con mutágenos químicos y físicos.

    Directory of Open Access Journals (Sweden)

    Willy Navarro Álvarez

    2016-03-01

    Full Text Available Plants of common bean were selected with an elongated hipocotyle from M3 population produced through embryos treatment with EMS and gamma rays from Cobalt-60 source. The mutants with elongated hipocotyle were reproduced to know the inheritance of this character.

  18. Transcriptome analysis of salt tolerant common bean (Phaseolus vulgaris L.) under saline conditions

    National Research Council Canada - National Science Library

    Hiz, Mahmut Can; Canher, Balkan; Niron, Harun; Turet, Muge

    2014-01-01

    .... Common bean, Phaseolus vulgaris L., a major protein source in developing countries, is highly affected by soil salinity and the information on genes that play a role in salt tolerance is scarce...

  19. The plant nitrogen mobilization promoted by Colletotrichum lindemuthianum in Phaseolus leaves depends on fungus pathogenicity

    National Research Council Canada - National Science Library

    Virginie Tavernier; Sandrine Cadiou; Karine Pageau; Richard Laugé; Michèle Reisdorf-Cren; Thierry Langin; Céline Masclaux-Daubresse

    2007-01-01

    .... In order to study nitrogen recycling and mobilization in host plants during pathogen attack and invasion, the Colletotrichum lindemuthianum/Phaseolus vulgaris interaction was used as a model. C...

  20. Los impactos del Tratado de Libre Comercio de América del Norte en la producción de frijol de Zacatecas

    Directory of Open Access Journals (Sweden)

    Elivier Reyes Rivas

    2007-01-01

    Full Text Available La apertura comercial total, prevista en el Tratado de Libre Comercio de América del Norte (TLCAN para 2008, puede afectar la agricultura y el empleo rural de Zacatecas, particularmente la producción de frijol, donde la entidad ocupa el primer lugar como productor nacional. Dicha leguminosa ocupa 57 por ciento del área sembrada en el estado y emplea a cerca de 70 mil personas. Al revisar los términos comerciales pactados, datos estadísticos y fuentes primarias, se concluye que la competitividad que genere la apertura comercial puede precipitar el abandono de los cultivos y el deterioro de las condiciones de vida de miles de agricultores. El gobierno mexicano debería reevaluar los términos del tratado para impulsar la producción agrícola y establecer políticas públicas nuevas generadoras de empleo.

  1. LOCI DE CARACTERES CUANTITATIVOS LIGADOS CON LA RESISTENCIA A MACROPHOMINA PHASEOLINA, FUSARIUM SP. Y SEQUÍA EN FRIJOL COMÚN.

    OpenAIRE

    MÉNDEZ AGUILAR, REINALDO

    2015-01-01

    El frijol común es originario de México. Es la fuente de proteínas más importante y constituye junto con el maíz la base de la alimentación de los mexicanos con menores ingresos. La sequía es la causa principal en la reducción de la producción de este grano básico seguido por las enfermedades entre las cuales destacan principalmente dos géneros de hongos causantes de pudriciones de la raíz y el tallo: Macrophomina y Fusarium. En la actualidad, con las herramientas de la biología molecular tal...

  2. Channel and hillslope processes revisited in the Arroyo de los Frijoles watershed near Santa Fe, New Mexico

    Science.gov (United States)

    Gellis, Allen C.; Emmett, William W.; Leopold, Luna B.

    2005-01-01

    Many of the original study sites were relocated and surveyed in the mid-1990's to determine subsequent channel and hillslope changes and to determine whether trends of channel and hillslope aggradation and degradation that were evident in the 1950's and 1960's have continued. In general, the net change in channel geometry has been small over the last 30n40 years. The average change in cross-sectional area of 32 resurveyed cross sections was erosion of 0.27 square meter, which equates to a 4-percent increase in cross-sectional area. The average net change in thalweg elevation for 51 resurveyed cross sections was degradation of 0.04 meter. Unpublished data (1964n68) from the scour chains showed that 371 chains had an average scour of 0.14 (+/-) 0.14 meter and that 372 chains showed an average fill of 0.13 + 0.11 meter. Scour, found in the original study (1958n64) to be proportional to the square root of discharge, was confirmed with the addition of unpublished data (1964?68). The observed channel changes have no consistent trend, compared either to results observed in the original 1966 study or to distance from the watershed divide. The conclusion drawn in the original study was that most channels were aggrading; the resurvey showed that aggradation did not continue. An increase in housing and population in the Arroyo de los Frijoles watershed since the 1950's has led to more roads. Channel degradation is most noticeable at road crossings. The greatest degradation of the main channel Arroyo de los Frijoles, 1.53 meters, and the greatest aggradation, 0.38 meter, occur downstream and upstream, respectively, from a culvert in a dirt road. Periods of high average annual rainfall intensity reported for Santa Fe for 1853?80 immediately preceded late 19th century arroyo incision, and another period of high-intensity rainfall began in 1967. This may indicate that climatic factors are again favorable for arroyo incision in this part of New Mexico; data from this resurvey

  3. Maximización física y económica del agua de riego en la producción del cultivo del frijol

    Directory of Open Access Journals (Sweden)

    Norge Tornés Olivera

    2016-01-01

    Full Text Available La investigación se desarrolló entre los meses de noviembre de 2013 a marzo de 2014 con el objetivo maximizar los niveles físicos y económicos de uso de agua en la producción del frijol mediante la simulación de los rendimientos con el modelo AquaCrop. El área de la investigación pertenece a la UBPC Grito de Yara, la cual se encuentra ubicada a los 20 0 25’ de Latitud N y a los 76 0 53’ de Longitud O con una altura de 6 m.s.n.m. Se realizó un total de 7 riegos. Se sembró la variedad Delicia 360, el día 20 de diciembre con un marco de plantación de 0,5 x 0,8 m. Una vez calibrado el modelo AquaCrop, se simularon los rendimientos del cultivo de frijol bajo diferentes niveles de agua disponible para obtener la función de producción con la cual se realizó la maximización física y económica del agua. El volumen de agua que garantiza un máximo físico es de 2 244,37 m 3 ·ha -1 para la obtención de rendimientos de 2 406,51 kg·ha -1 . El volumen de agua que garantiza el máximo económico es de 2 438,06 m 3 ·ha -1 con lo cual se garantiza una ganancia de 47 .38 $·ha -1.

  4. REACCIÓN DE VARIEDADES DE FRIJOL A LA INOCULACIÓN ARTIFICIAL DE Aphelenchoides besseyi, AGENTE CAUSAL DE LA FALSA MANCHA ANGULAR

    Directory of Open Access Journals (Sweden)

    Walter Barrantes-Santamar\\u00EDa

    2008-01-01

    Full Text Available Especímenes adultos y juveniles de A. besseyi provenientes de cultivo in vitro se inocularon sobre el follaje de plantas de frijol de las variedades Bribri, Huetar, Telire y Chirripó Rojo. Las edades de las plantas inoculadas en el invernadero fueron de diez, 20 y 30 días después de siembra (dds. Los bioensayos se realizaron en los invernaderos de la Escuela de Ciencias Agrarias de la Universidad Nacional, Heredia-Costa Rica en el 2006. El objetivo de esta investigación fue evaluar la reacción de variedades de frijol al nematodo A. besseyi. Las variedades estudiadas mostraron similar nivel de resistencia al ataque del nematodo, de acuerdo a la escala de severidad utilizada; sin embargo, las variedades Huetar y Bribri mostraron menor severidad de infección y población de nematodos que las otras dos variedades. Los primeros síntomas de la enfermedad se desarrollaron en las hojas primarias. El período de incubación fue más corto a medida que las inoculaciones se realizaron en plantas de mayor edad. Igualmente, las hojas maduras fueron más susceptibles que las jóvenes a la infección del nematodo. El desarrollo de la enfermedad difi rió entre hojas primarias y trifoliadas y dependió de la disponibilidad de tejido foliar, la madurez del follaje y la producción de inóculo secundario.

  5. 78 FR 53783 - Notice of Intent To Repatriate Cultural Items: Colorado College, Colorado Springs, CO

    Science.gov (United States)

    2013-08-30

    ... National Park Service Notice of Intent To Repatriate Cultural Items: Colorado College, Colorado Springs, CO..., Chief of Staff, President's Office, Colorado College, 14 E. Cache La Poudre, Colorado Springs, CO 80903... Springs, CO, that meet the definition of unassociated funerary objects under 25 U.S.C. 3001. This...

  6. 78 FR 19304 - Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs, CO

    Science.gov (United States)

    2013-03-29

    ... National Park Service Notice of Intent To Repatriate Cultural Items: The Colorado College, Colorado Springs... College, Armstrong Hall, Room 201, 14 E. Cache La Poudre, Colorado Springs, CO 80903, telephone (719) 389... Center (formerly known as the Taylor Museum and the Colorado Springs Fine Arts Center) and the...

  7. Crecimiento y contenido de prolina y carbohidratos de plántulas de frijol sometidas a estrés por sequía Growth and, proline and carbohydrate content of bean seedlings subjected to drought stress

    OpenAIRE

    Teresa Susana Herrera Flores; Joaquín Ortíz Cereceres; Adriana Delgado Alvarado; Jorge Alberto Acosta Galleros

    2012-01-01

    El estrés por sequía en el altiplano de México, es un factor limitante en el rendimiento del frijol. En el presente trabajo se estudiaron las respuestas fisiológicas (crecimiento) y bioquímicas (carbohidratos solubles y prolina) en la radícula de plántulas de frijol resistente, Pinto Villa, y susceptible a la sequía Bayo Madero, ambas de la raza Durango, para identificar características rápidas de medir que sean confiables para selección por resistencia a estrés por sequía. La radícula de las...

  8. Symbiont shift towards Rhizobium nodulation in a group of phylogenetically related Phaseolus species.

    Science.gov (United States)

    Servín-Garcidueñas, Luis E; Zayas-Del Moral, Alejandra; Ormeño-Orrillo, Ernesto; Rogel, Marco A; Delgado-Salinas, Alfonso; Sánchez, Federico; Martínez-Romero, Esperanza

    2014-10-01

    Bean plants from the Phaseolus genus are widely consumed and represent a nitrogen source for human nutrition. They provide biological fertilization by establishing root nodule symbiosis with nitrogen-fixing bacteria. To establish a successful interaction, bean plants and their symbiotic bacteria need to synchronize a proper molecular crosstalk. Within the Phaseolus genus, P. vulgaris has been the prominent species to study nodulation with Rhizobium symbionts. However the Phaseolus genus comprises diverse species whose symbionts have not been analyzed. Here we identified and studied nodule bacteria from representative Phaseolus species not previously analyzed and from all the described wild species related to P. vulgaris. We found Bradyrhizobium in nodules from most species representing all Phaseolus clades except in five phylogenetically related species from the P. vulgaris clade. Therefore we propose that Bradyrhizobium nodulation is common in Phaseolus and that there was a symbiont preference shift to Rhizobium nodulation in few related species. This work sets the basis to further study the genetic basis of this symbiont substitution.

  9. Survey for bats in Jackson County, Colorado

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report covers a targeted bat survey of Jackson County in north-central Colorado to better understand the abundance and distribution of bats in Colorado. The...

  10. Volatile compounds of dry beans (Phaseolus vulgaris L.).

    Science.gov (United States)

    Oomah, B Dave; Liang, Lisa S Y; Balasubramanian, Parthiba

    2007-12-01

    Volatile compounds of uncooked dry bean (Phaseolus vulgaris L.) cultivars representing three market classes (black, dark red kidney and pinto) grown in 2005 were isolated with headspace solid phase microextraction (HS-SPME), and analyzed with gas chromatography mass spectrometry (GC-MS). A total of 62 volatiles consisting of aromatic hydrocarbons, aldehydes, alkanes, alcohols and ketones represented on average 62, 38, 21, 12, and 9 x 10(6) total area counts, respectively. Bean cultivars differed in abundance and profile of volatiles. The combination of 18 compounds comprising a common profile explained 79% of the variance among cultivars based on principal component analysis (PCA). The SPME technique proved to be a rapid and effective method for routine evaluation of dry bean volatile profile.

  11. Phaseolus vulgaris RbohB functions in lateral root development

    Science.gov (United States)

    Montiel, Jesús; Arthikala, Manoj-Kumar; Quinto, Carmen

    2013-01-01

    Respiratory burst oxidase homologs (RBOHs) catalyze the reduction of oxygen to generate superoxide anion, a kind of reactive oxygen species (ROS). The ROS produced by RBOHs play essential roles in diverse processes, such as root hair development, stomata closure and signaling mechanisms in response to abiotic stimuli and during plant-pathogen interactions. Recently, we found that PvRbohB silencing in transgenic Phaseolus vulgaris roots had a negative impact on lateral root density. In this work, we show that the downregulation of PvRbohB affects both the growth and ROS levels in recently emerged lateral roots. In addition, we found that the PvRbohB promoter was activated during lateral root primordium initiation in the pericycle, and remained active throughout lateral root development. This study identifies RBOHs as potentially important players in lateral root development in P. vulgaris. PMID:23221754

  12. Injury response of Phaseolus vulgaris to ozone flux density

    Science.gov (United States)

    Amiro, B. D.; Gillespie, T. J.; Thurtell, G. W.

    This study describes a quantitative relationship between mean O 3 flux density and the length of exposure needed for the occurrence of visual injury to Phaseolus vulgaris L. Similar relationships were found for 14 day old and 6 week old plants using a whole leaf gas exchange cuvette system. Cultivars Seafarer (O 3 sensitive) and Gold Crop (O 3 resistant) exhibited similar responses at flux densities > 3 mg m -2 h -1 but only Seafarer was injured below this flux density. O 3 concentration and length of exposure period alone did not contain sufficient information to describe the onset of visual foliar injury. The use of O 3 concentrations in excess of normal ambient conditions compensated for low leaf conductances so that flux densities in the cuvette were similar to those found in the field.

  13. Influencia del mulch en los índices de crecimiento del frijol variedad «Bat-304»

    Directory of Open Access Journals (Sweden)

    Guillermo Hernández del Valle

    2008-01-01

    Full Text Available El empleo de coberturas, arropes o mulch es un factor importante en climas donde la evapotranspiración es elevada ya que su uso favorece la disminución de la temperatura del suelo y mantiene una humedad necesaria a la planta reduciendo la pérdida de agua por evaporación. Con el objetivo de determinar la influencia del mulch en índices crecimiento del fríjol (Phaseolus vulgaris L. de grano negro var. Bat-304, se realizó un experimento en las áreas agrícolas de la Facultad de Agronomía durante los meses de enero a abril de 2005 sobre un suelo Ferralítico Rojo típico. Los restos vegetales utilizados para el arrope se distribuyeron uniformemente sobre la superficie de las parcelas antes de la siembra, equivalente a 4, 5; 7; 5 y 15 t·ha-1 y un control sin mulch. Los tratamientos que recibieron los beneficios del mulch alcanzaron valores de índices de crecimiento significativamente superiores al tratamiento sin mulch, en cada fase fenológica, destacándose la aplicación equivalente a 15 t·ha-1 como el mejor resultado.

  14. Rojo INIFAP, nueva variedad de frijol de grano rojo para el trópico de México Rojo INIFAP, a new red grain bean variety for the Tropic of Mexico

    Directory of Open Access Journals (Sweden)

    Bernardo Villar Sánchez

    2010-12-01

    Full Text Available En los Distritos de Desarrollo Rural, Centro y Altos de la Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación del estado de Chiapas, existen áreas donde se siembran materiales criollos de frijol de grano rojo, los cuales se comercializan tanto en los mercados locales de las comunidades, como en la capital del estado, donde también se comercializa grano de frijol rojo proveniente de Centroamérica. Aunque estos genotipos tienen adaptación específica, son de bajo rendimiento potencial y susceptible a enfermedades. En esta entidad existe un creciente interés por parte de los agricultores por la siembra de este tipo de grano, lo cual obedece al establecimiento cada vez mayor de inmigrantes centroamericanos en el estado, que culturalmente consumen frijol de grano rojo y por lo tanto aumenta la demanda local de este tipo de frijol. Por otra parte, al incrementar la superficie de siembra y el rendimiento de frijol de grano rojo, representa una oportunidad para el estado de Chiapas de exportar este tipo de grano, considerando la demanda que hay en El Salvador, Honduras y Nicaragua, así como en los Estados Unidos de América, donde radican habitantes de estos países centroamericanos, además de promover su consumo en el país.In the Rural Development Districts (DDR, Centro y Altos of Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA in the State of Chiapas, there are areas where creole materials of red grain bean are sowed, which are traded in local markets where they are produced, as in those of State capital, where it is also traded red grain bean from Central America. Although these genotypes have specific adaptation, they are from low yield potential and susceptible to diseases. In this state there is a growing interest in the farmers for sowing of this type of grain, which stands for the increasing establishment of Central American immigrants in the state that culturally

  15. Diversidad de faseolinas en frijol común cultivado del Caribe Phaseolin diversity in cultivated common bean from the Caribbean

    Directory of Open Access Journals (Sweden)

    Matthew W. Blair

    2007-01-01

    Full Text Available Se evaluó el tipo de faseolina en 180 accesiones cultivadas de Phaseolus vulgaris del Caribe de la colección del Centro Internacional de Agricultura Tropical (CIAT. En fríjoles de Cuba (25 accesiones predominó el tipo S (68%, semilla pequeña, roja o negra y hábito de crecimiento de tipos II y III, seguidos por fríjoles con faseolina T (20 % y con un bajo porcentaje los tipos B, CH y Sd. En Haití (108 accesiones las faseolinas más comunes fueron T (48.1%, semilla grande, rojo moteado y hábito arbustivo y S (42.6%, semilla pequeña, negra, y unas pocas mostraron faseolinas B, Sb y Sd. En los fríjoles de Puerto Rico (21 accesiones las faseolinas más comunes fueron T (57.14%, semilla grande, rojo moteada y S (42.86%, semilla pequeña amarilla, negra o blanca. En los de República Dominicana (21 accesiones abundó el tipo T (85.7%, semilla grande o mediana rojomoteada. En los fríjoles de Jamaica (5 predominaron las faseolinas T y B (40% de cada una. Los patrones de faseolina indicaron la prevalencia tanto de fríjoles Mesoamericanos como Andinos.In this study, 180 genotypes of cultivated Phaseolus vulgaris from the Caribbean were evaluated with the phaseolin marker. All the accessions belonged to the germplasm collection of the International Center for Tropical Agriculture (CIAT. Beans from Cuba were mostly S phaseolin (68.0 % type, with small seed size, red and black seed color and type II or type III growth habit. These were followed by beans with T phaseolin (20.0 % and finally by those with B, CH and Sd phaseolin. In Haiti , the most common phaseolin was T (48.1%, the majority of which were large seeded bush beans, mostly of the red mottled seed color class. Many of the remaining accessions were S phaseolin (42.6% and had small black seed, although a few had B, Sb and Sd phaseolin (4.6, 2.8 and 1.9% of genotypes, respectively. In Puerto Rico, T phaseolin was in the majority with 57.1%, most of these with large red or red mottled seed

  16. Phaseolin diversity in cultivated common bean from the Caribbean Diversidad de faseolinas en frijol común cultivado del Caribe

    Directory of Open Access Journals (Sweden)

    Hidalgo Rigoberto

    2007-12-01

    Full Text Available In this study, 180 genotypes of cultivated Phaseolus vulgaris from the Caribbean were evaluated with the phaseolin marker. All the accessions belonged to the germplasm collection of the International Center for Tropical Agriculture (CIAT. Beans from Cuba were mostly S phaseolin (68.0 % type, with small seed size, red and black seed color and type II or type III growth habit. These were followed by beans with T phaseolin (20.0 % and finally by those with B, CH and Sd phaseolin. In Haiti, the most common phaseolin was T (48.1%, the majority of which were large seeded bush beans, mostly of the red mottled seed color class. Many of the remaining accessions were S phaseolin (42.6% and had small black seed, although a few had B, Sb and Sd phaseolin (4.6, 2.8 and 1.9% of genotypes, respectively. In Puerto Rico, T phaseolin was in the majority with 57.1%, most of these with large red or red mottled seed. S phaseolin was represented by 42.9 % of landraces some of which had small yellow, black or white seed. In the Dominican Republic, the T phaseolin was very abundant (85.7% the majority of which were medium to large seeded bush beans all of which were red mottled. A smaller proportion of Dominican landraces had S and B phaseolin (9.5% and 4.8% respectively. Finally, in Jamaica, T phaseolin was found for 40.0 % of the landraces, B phaseolin was equally common and 20% of the landraces had S phaseolin although this was based on a small sample of genotypes. The phaseolin patterns indicated the prevalence of both Andean and Mesoamerican beans in the Caribbean.

    Se evaluó el tipo de faseolina en 180 accesiones cultivadas de Phaseolus vulgaris del Caribe de la colección del Centro Internacional de Agricultura Tropical (CIAT

  17. Compuestos nitrogenados indicadores de estrés en respuesta a las dosis tóxicas y deficientes de Nitrógeno en frijol ejotero

    Directory of Open Access Journals (Sweden)

    Esteban Sánchez

    2016-01-01

    Full Text Available Introducción: Un amplio rango de estreses ambientales, tales como baja temperatura, sequía, alcalinidad, salinidad, deficiencia y toxicidad de nutrientes son potencialmente dañinos para las plantas. El papel del nitrógeno como nutriente esencial y componente estructural de aminoácidos, proteínas, ácidos nucleicos y otros constituyentes esenciales para el desarrollo ha sido ampliamente documentado en varias especies debido a la importancia en los procesos de crecimiento y producción agrícola. Sin embargo, en la actualidad, existe escasa literatura del efecto de la deficiencia y toxicidad de nitrógeno sobre los compuestos osmoreguladores como indicadores de estrés en plantas. Por lo que el objetivo del presente trabajo fue estudiar los compuestos nitrogenados indicadores de estrés (prolina, glicinabetaina y colina en respuesta a las dosis tóxicas y deficientes de N en frijol ejotero desarrollado en cámara de cultivo bajo condiciones controladas y sistema hidropónico. Método: El nitrógeno fue aplicado a la solución nutritiva en la forma de NH4NO3 y en dosis crecientes: N1 = 1.5 mM, N2 = 3.0 mM, N3 = 6.0 mM, N4 = 12.0 mM, N5 = 18.0 mM y N6 = 24.0 mM de N. Los parámetros analizados fueron la acumulación de biomasa, la concentración de prolina, glicinabetaina y colina en hojas, raíces, semillas y vainas de frijol ejotero cv. Strike. Resultados: La aplicación de dosis deficientes y tóxicas de N afectó la producción de biomasa en frijol, siendo las dosis tóxicas las que afectaron más este parámetro. Por otro lado, resaltar que los osmoreguladores prolina, glicinabetaina y colina solamente se acumularon bajo condiciones de toxicidad de N (N6, sin embargo, en condiciones de estrés provocado por la deficiencia de N (N1 no se produce la acumulación de estos compuestos. Discusión o Conclusión: Los compuestos nitrogenados indicadores de estrés solamente se acumulan bajo condiciones de toxicidad de N (N6, sin embargo en

  18. Reacción de germoplasma de frijol común a macrophomina phaseolina en condiciones de riego-secano en Veracruz, México

    Directory of Open Access Journals (Sweden)

    Netzahualcoyotl Mayek

    2004-01-01

    Full Text Available Reacción de germoplasma de frijol común a Macro phomina phaseolina en condiciones de riego-secano en Veracruz, México. Se establecieron cuatro experimentos en Isla y Cotaxtla, Veracruz, México con los objetivos de determinar la reacción de 56 genotipos de frijol común a M. phaseolina bajo condiciones de riego-secano y para identificar germoplasma resistente al hongo y con estabilidad del rendimiento bajo condiciones de deficiencia hídrica. El germoplasma evaluado incluyó diferentes orígenes, razas genéticas, colores de la testa de la semilla, variedades y líneas experimentales. Se aleatorizó en diseños experimentales látice 7x8 cultivado bajo condiciones de riego y de secano (suspensión del riego en la fase reproductiva. El déficit de humedad adelantó la madurez fisiológica e incrementó la severidad de la pudrición carbonosa; además de reducir el rendimiento de grano. En promedio de los cuatro experimentos, TLP20/NT81, NGO 99165, Negro Veracruz, A 774 y BAT 477 mostraron los mayores rendimientos de grano (más de 1000 kg/ha y los promedios menores de severidad de la pudrición carbonosa; mientras que Negro Otomí, 9457-43, NGO 99176, CNC 2 y VAX 2 exhibieron un comportamiento inverso. La intensidad de la sequía en cada experimento fue variable y los valores mayores se observaron en Isla (0,53 y 0,38 en 2000 y 2001, respectivamente. No hubo asociación clara entre el índice de susceptibilidad a la sequía y la severidad de la pudrición carbonosa o rendimiento de grano, aunque si relación negativa entre rendimiento de grano y severidad de la pudrición carbonosa (r = -0,26* en promedio. No obstante lo anterior, el germoplasma con menores daños por M. phaseolina y mayores rendimientos de grano mostró, en general, valores menores de índice de susceptibilidad a la sequía.

  19. Diversidad de frijoles nativos de diferentes regiones del estado de Puebla Native beans diversity of different regions from state of Puebla

    Directory of Open Access Journals (Sweden)

    Ana Rosa Ramírez-Pérez

    2012-06-01

    Full Text Available El frijol común representa un cultivo básico en México con una gran variabilidad en sus características morfológicas. En estudios sobre la diversidad genética se han usado marcadores moleculares, entre los que se encuentran los RAPD. El presente trabajo tuvo como objetivo caracterizar mediante caracteres morfológicos, agronómicos, de calidad y marcadores RAPD, variedades de frijoles nativos cultivadas en diferentes comunidades del estado de Puebla. La extracción de ADN se realizó en una muestra de hojas jóvenes de las variedades sembradas bajo invernadero. Se utilizaron 7 iniciadores RAPDs de la serie OPERON Technologies. Las bandas polimórficas se codificaron por presencia y ausencia. Con el programa NTSYS pc 2.2 se generó un dendograma empleando el índice de similitud de Dice, con nivel de corte a un valor de 0.48, donde se definieron 13 grupos. Seis grupos estuvieron integrados por una variedad, 4 grupos por 2 variedades, 2 grupos por 3 variedades y un grupo formado por 7 variedades. Los grupos definidos se integraron por colores de grano distintos y coincidieron en otras características morfológicas como días a floración, tiempo de cocción y contenido de proteína.Common bean is basic crop in Mexico with great variability in its morphological characteristics. RAPD is among molecular markers, which have been used in studies about genetic diversity. The aim of this work was to characterize by morphological, agronomical, quality characters and RAPD markers the varieties harvested in different localities from state of Puebla. DNA extraction was made from sample of young leaves of varieties planted under greenhouse. 7 RAPDs starters from OPERON Technologies were used. Polymorphical bands were coded by presence and absence. With NTSYS pc 2.2 software dendrogram was created using Dice's similarity index, with cut level at value of 0.48 where 13 groups were defined. Six groups were comprised by single variety, 4 groups by 2

  20. An antifungal defensin from Phaseolus vulgaris cv. 'Cloud Bean'.

    Science.gov (United States)

    Wu, Xiangli; Sun, Jian; Zhang, Guoqing; Wang, Hexiang; Ng, Tzi Bun

    2011-01-15

    An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 μM. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 μM. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 μM. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 μM and 40 μM, respectively.

  1. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean

    Institute of Scientific and Technical Information of China (English)

    Yau Sang Chan; Jack Ho Wong; Evandro Fei Fang; Wen Liang Pan; Tzi Bun Ng

    2012-01-01

    A 5.4-kDa antifungal peptide,with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC5o=3 μM),Setospaeria turcica and Bipolaris maydis,was isolated from the seeds of Phaseolus vulgaris cv.brown kidney bean.The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75.The antifungal activity of the peptide against M.arachidicola was stable in the pH range 3-12 and in the temperature range 0℃ to 80℃.There was a slight reduction of the antifungal activity at pH 2 and 13,and the activity was indiscernible at pH 0,1,and 14.The activity at 90℃ and 100℃ was slightly diminished.Deposition of Congo red at the hyphal tips of M.arachidicola was induced by the peptide indicating inhibition of hyphal growth.The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells,in contrast to the presence of such activity of other antifungal peptides,indicates that different domains are responsible for the antifungal and antiproliferative activities.

  2. An antifungal peptide from Phaseolus vulgaris cv. brown kidney bean.

    Science.gov (United States)

    Chan, Yau Sang; Wong, Jack Ho; Fang, Evandro Fei; Pan, Wen Liang; Ng, Tzi Bun

    2012-04-01

    A 5.4-kDa antifungal peptide, with an N-terminal sequence highly homologous to defensins and inhibitory activity against Mycosphaerella arachidicola (IC(50)= 3 μM), Setospaeria turcica and Bipolaris maydis, was isolated from the seeds of Phaseolus vulgaris cv. brown kidney bean. The peptide was purified by employing a protocol that entailed adsorption on Affi-gel blue gel and Mono S and finally gel filtration on Superdex 75. The antifungal activity of the peptide against M. arachidicola was stable in the pH range 3-12 and in the temperature range 0°C to 80°C. There was a slight reduction of the antifungal activity at pH 2 and 13, and the activity was indiscernible at pH 0, 1, and 14. The activity at 90°C and 100°C was slightly diminished. Deposition of Congo red at the hyphal tips of M. arachidicola was induced by the peptide indicating inhibition of hyphal growth. The lack of antiproliferative activity of brown kidney bean antifungal peptide toward tumor cells, in contrast to the presence of such activity of other antifungal peptides, indicates that different domains are responsible for the antifungal and antiproliferative activities.

  3. Landscape genetics, adaptive diversity and population structure in Phaseolus vulgaris.

    Science.gov (United States)

    Rodriguez, Monica; Rau, Domenico; Bitocchi, Elena; Bellucci, Elisa; Biagetti, Eleonora; Carboni, Andrea; Gepts, Paul; Nanni, Laura; Papa, Roberto; Attene, Giovanna

    2016-03-01

    Here we studied the organization of genetic variation of the common bean (Phaseolus vulgaris) in its centres of domestication. We used 131 single nucleotide polymorphisms to investigate 417 wild common bean accessions and a representative sample of 160 domesticated genotypes, including Mesoamerican and Andean genotypes, for a total of 577 accessions. By analysing the genetic spatial patterns of the wild common bean, we documented the existence of several genetic groups and the occurrence of variable degrees of diversity in Mesoamerica and the Andes. Moreover, using a landscape genetics approach, we demonstrated that both demographic processes and selection for adaptation were responsible for the observed genetic structure. We showed that the study of correlations between markers and ecological variables at a continental scale can help in identifying local adaptation genes. We also located putative areas of common bean domestication in Mesoamerica, in the Oaxaca Valley, and the Andes, in southern Bolivia-northern Argentina. These observations are of paramount importance for the conservation and exploitation of the genetic diversity preserved within this species and other plant genetic resources.

  4. Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride.

    Science.gov (United States)

    Tejera García, Noel A; Iribarne, Carmen; Palma, Francisco; Lluch, Carmen

    2007-08-01

    Changes in catalase activity during the development of the Rhizobium-legume symbiosis as well as its response in salinized plants of Phaseolus vulgaris and Medicago sativa, was studied. Besides, it was examined the behavior of the enzyme, isolated from leaves and root nodules, during in vitro incubation with NaCl doses. Nodule catalase activities of both legumes were assayed with several enzyme inhibitors and also purified. Leaf catalase activity of Phaseolus vulgaris and Medicago sativa decreased and increased respectively throughout the ontogeny, but root nodule catalase kept a high and stable value. This last result suggests that both legumes require the maintenance of high nodule catalase in nitrogen-fixing nodules. Under salt stress conditions leaf and nodule catalase activity decreased in both, grain and pasture legumes. Because catalase from leaf of Medicago sativa and nodules of Phaseolus vulgaris were relatively sensitive to NaCl during in vitro experiments, the detoxifying role of this enzyme for H(2)O(2) should be limited in such conditions. Both catalases, from determinate and indeterminate nodules, were affected neither by oxygen nor superoxide radicals but showed a strong (Phaseolus vulgaris) or partial (Medicago sativa) inhibition with dithiothreitol, dithionite and beta-mercaptoethanol. Besides, cyanide was the most potent inhibitor of nodule catalases. Finally, catalases partially purified by immobilized metal ion affinity chromatography migrated at 42 (Phaseolus vulgaris) and 46kDa (Medicago sativa) on SDS-PAGE, whereas native forms on sephacryl S-300 columns exhibited a molecular mass of 59 and 48kDa (Phaseolus vulgaris) and 88 and 53kDa (Medicago sativa).

  5. Los 'Colorados': Etnohistoria y Toponimia

    NARCIS (Netherlands)

    Gómez-Rendón, J.

    2015-01-01

    Los "colorados" comprendían varios grupos étnicos emparentados etnolingüísticamente que ocupaban el piedemonte andino occidental desde El Carchi hasta Bolívar así como las tierras bajas del Pacífico en los sistemas hidrográficos de los ríos Esmeraldas y Guayas. Aunque la ocupación "colorada" de

  6. Los 'Colorados': Etnohistoria y Toponimia

    NARCIS (Netherlands)

    Gómez-Rendón, J.

    2015-01-01

    Los "colorados" comprendían varios grupos étnicos emparentados etnolingüísticamente que ocupaban el piedemonte andino occidental desde El Carchi hasta Bolívar así como las tierras bajas del Pacífico en los sistemas hidrográficos de los ríos Esmeraldas y Guayas. Aunque la ocupación "colorada" de esta

  7. 75 FR 58426 - Notice of Inventory Completion: The Colorado College, Colorado Springs, CO; Correction

    Science.gov (United States)

    2010-09-24

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR National Park Service Notice of Inventory Completion: The Colorado College, Colorado Springs, CO... College, Colorado Springs, CO. The human remains and associated funerary objects were removed from...

  8. Resistant starch and in vitro starch digestibility of cooked “ayocote” bean (phaseolus coccineous)

    OpenAIRE

    2004-01-01

    “Ayocote” beans (Phaseolus coccineous) were cooked and studied regarding their chemical composition and in vitro starch digestibility. Protein and ash contents were 20.46 and 1.39%, respectively, which are among the lowest levels for seeds of the Phaseolus genus. On the contrary, lipid content was relatively high (3.31%). Available starch (AS) values decreased with storage at 4ºC, changing from 37.93 (freshly cooked “control” seeds) to 32.18% (seeds stored for 96h). An inverse pattern was fou...

  9. Evanescencias en las traducciones del Chilam Balam de Chumayel: el maíz y el frijol del Sur, los ibes del Oriente y el pozol ritual

    Directory of Open Access Journals (Sweden)

    Miguel Mauricio Vassallo Rodríguez

    2015-12-01

    Full Text Available Este trabajo intenta restituir a las traducciones del maya el sentido original de algunos párrafos de la primera página del Chilam Balam de Chumayel, que fueron alterados en algunas ediciones de este libro. Los parágrafos contenidos al inicio del texto reflejan la concepción de que al comienzo de esta Era los elementos del axis mundi se desdoblaron en los cuadrantes del cosmos, replicándose pero adquiriendo el color y ciertas características inherentes a cada uno de los cuatro rumbos del cosmos. Algunas traducciones y ediciones omitieron al maíz y/o al frijol del rumbo sur y a los ibes del oriente; esto mutila el sentido general de esa parte del texto original, donde dichos elementos están indubitablemente presentes. También intento restituir la presencia del pozol ritual (saka’ y del tzune en dos de las interrogantes de Zuyua than que han sido erróneamente identificados como "atole" en todas las ediciones del Chumayel.

  10. Diversidad de razas de colletotrichum lindemuthianum en Antioquía y evaluación de germoplasma de frijol crema-rojo por resistencia a antracnosis

    Directory of Open Access Journals (Sweden)

    Gloria E. Santana

    2002-01-01

    Full Text Available Para verificar si la composición de razas en el Oriente de Antioquía se caracterizaron 15 aislamientos de C. lindemuthianum, usando 12 variedades diferenciales de frijol aceptadas internacionalmente. Se identificaron 11 razas, seis de las cuales (9, 131, 135, 139, 643, y 645 fueron caracterizadas por primera vez. Además, se evaluaron 49 genotipos procedentes de los bancos de germoplasma del CIAT y CORPOICA y cultivares de agricultor de municipios productores. Los genotipos fueron evaluados en campo y en invernadero con las razas más virulentas del patógeno (651 y 653 encontradas en Antioquía. Sólo cuatro genotipos (AND1084, SUG130, LAS106 y DiacolCatio mostraron resistencia a ambas razas. Estos genotipos podrían usarse como parentales para un plan de cruzas, con el fin de introducir genes de resistencia a variedades de importancia comercial que presenten susceptibilidad a la antracnosis.

  11. Análisis de la tecnología local de producción de frijol caraota (Phaseolus vulgaris L. en la zona de vega del rio Arauca Local technology analysis of black bean production (Phaseolus vulgaris L. in area shore of the Arauca river

    Directory of Open Access Journals (Sweden)

    Valderrama N. Yhovana

    1997-12-01

    Full Text Available El cultivo del fríjol negro o caraota constituye un importante renglón agrícola de impacto económico y social en el municipio de Arauquita (Arauca, donde cerca de 500 hectáreas se destinan para su siembra en los mesessecos de Octubre y Noviembre. En este municipio la caraota es un cultivo tradicional, en el cual se emplea poca tecnología y existe poca investigación. El producto se comercializa, principalmente, en Venezuela donde existe una alta demanda. Para analizar la Tecnología Local de Producción (TLP, se realizaron encuestas entre agricultores representativos en diferentes veredas del municipio de Arauquita, encontrándose que es un cultivo en donde la mano de obra es contratada con personal de la región y cuyas labores se resumen en: siembra"a chuzo" sobre terrenos previamente quemados, el control de malezas, y finalmente cosecha y beneficio.
    En la cadena de comercialización entre Colombia y Venezuela existen por lo menos 7 pasos entre el productor y el consumidor final.
    The black bean crop is an important economic and social activity in Arauquita (east of Colombia. In thal region, about 500 hectares are devoted to planting seeds in dry months (October-November. This is a traditional crop, wich used not much technology and there is little research. The product is marketed mainly in Venezuela, where it has high demando This study was carried out making surveys to representative farmers on the region. In the black bean crop the farms work are contracted with personal of the region and its agricultural practices in summary are: burn of the vegetation, sowing, wich is done to random hill ("chuzo", putting several seeds by hill. Except the control of weeds, no fitosanitary control is done, isn't fertilized and its harvest and thresh are manual. Marketing between Colombia and Venezuela has at least seven steps from the producer to final costumer.

  12. Caracterización de la Producción de Frijol (Vigna sp.) en los Municipios Turén y Santa Rosalía del Estado Portuguesa (2000-2005)

    OpenAIRE

    Torres, Andrew; Paredes, Franklin

    2010-01-01

    En esta investigación se caracteriza la producción de frijol (Vigna sp.) en los Municipios Turén y Santa Rosalía del estado Portuguesa. Se aplicó una encuesta a los productores de la Unidad de Estudio, luego se determinaron diversas estadísticas descriptivas. Se aplicó un Análisis Factorial de Componentes Principales (AFCP) a fin de identificar variables subyacentes. Entre los resultados destacan: 1) los productores consideran importante la integración empresarial, sugiriendo que existe una g...

  13. Diversification and population structure in common beans (Phaseolus vulgaris L..

    Directory of Open Access Journals (Sweden)

    Matthew W Blair

    Full Text Available Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild beans and landraces is important for the proper utilization and conservation of these important genetic resources. The goal of this research was to evaluate a collection of wild beans with fluorescent microsatellite or simple sequence repeat markers and to determine the population structure in combination with cultivated beans of all known races. Marker diversity in terms of average number of alleles per marker was high (13 for the combination of 36 markers and 104 wild genotypes that was similar to the average of 14 alleles per marker found for the 606 cultivated genotypes. Diversity in wild beans appears to be somewhat higher than in cultivated beans on a per genotype basis. Five populations or genepools were identified in structure analysis of the wild beans corresponding to segments of the geographical range, including Mesoamerican (Mexican, Guatemalan, Colombian, Ecuadorian-northern Peruvian and Andean (Argentina, Bolivia and Southern Peru. The combined analysis of wild and cultivated accessions showed that the first and last of these genepools were related to the cultivated genepools of the same names and the penultimate was found to be distinct but not ancestral to the others. The Guatemalan genepool was very novel and perhaps related to cultivars of race Guatemala, while the Colombian population was also distinct. Results suggest geographic isolation, founder effects or natural selection could have created the different semi-discrete populations of wild beans and that multiple domestications and introgression were involved in creating the diversity of

  14. Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem.

    Science.gov (United States)

    Padder, Bilal A; Kamfwa, Kelvin; Awale, Halima E; Kelly, James D

    2016-01-01

    Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C. lindemuthianum. From this, we identified 3,250 significantly differentially expressed genes (DEGs) within and between the NILs over the time course of infection. During the biotrophic phase the majority of DEGs were up regulated in the susceptible NIL, whereas more DEGs were up-regulated in the resistant NIL during the necrotrophic phase. Various defense related genes, such as those encoding PR proteins, peroxidases, lipoxygenases were up regulated in the resistant NIL. Conversely, genes encoding sugar transporters were up-regulated in the susceptible NIL during the later stages of infection. Additionally, numerous transcription factors (TFs) and candidate genes within the vicinity of the Co-1 locus were differentially expressed, suggesting a global reprogramming of gene expression in and around the Co-1 locus. Through this analysis, we reduced the previous number of candidate genes reported at the Co-1 locus from eight to three. These results suggest the dynamic nature of P. vulgaris-C. lindemuthianum interaction at the transcriptomic level and reflect the role of both pathogen and effector triggered immunity on changes in plant gene expression.

  15. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection.

    Science.gov (United States)

    Oblessuc, Paula Rodrigues; Borges, Aline; Chowdhury, Bablu; Caldas, Danielle Gregório Gomes; Tsai, Siu Mui; Camargo, Luis Eduardo Aranha; Melotto, Maeli

    2012-01-01

    The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.). Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST) to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO) analysis indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For instance, cytokinin and ethylene responses were up-regulated and jasmonic acid, gibberellin, and abscisic acid responses were down-regulated, indicating that these hormones may play a central role in this pathosystem. Importantly, we have identified putative bean gene orthologs of Arabidopsis genes involved in the plant immune system. Based on experimental validation of gene expression, we propose that hypersensitive reaction as part of effector-triggered immunity may operate, at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes involved in pathogen perception and downstream signaling. We have identified specific bean genes and uncovered metabolic processes and pathways that may be involved in the immune response against pathogens. Our transcriptome database is a rich resource for mining novel defense-related genes, which enabled us to develop a model of the molecular components of the bean innate immune system regulated upon

  16. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection.

    Directory of Open Access Journals (Sweden)

    Paula Rodrigues Oblessuc

    Full Text Available BACKGROUND: The genus Colletotrichum is one of the most economically important plant pathogens, causing anthracnose on a wide range of crops including common beans (Phaseolus vulgaris L.. Crop yield can be dramatically decreased depending on the plant cultivar used and the environmental conditions. This study aimed to identify potential genetic components of the bean immune system to provide environmentally friendly control measures against this fungus. METHODOLOGY AND PRINCIPAL FINDINGS: As the common bean is not amenable to reverse genetics to explore functionality and its genome is not fully curated, we used putative Arabidopsis orthologs of bean expressed sequence tag (EST to perform bioinformatic analysis and experimental validation of gene expression to identify common bean genes regulated during the incompatible interaction with C. lindemuthianum. Similar to model pathosystems, Gene Ontology (GO analysis indicated that hormone biosynthesis and signaling in common beans seem to be modulated by fungus infection. For instance, cytokinin and ethylene responses were up-regulated and jasmonic acid, gibberellin, and abscisic acid responses were down-regulated, indicating that these hormones may play a central role in this pathosystem. Importantly, we have identified putative bean gene orthologs of Arabidopsis genes involved in the plant immune system. Based on experimental validation of gene expression, we propose that hypersensitive reaction as part of effector-triggered immunity may operate, at least in part, by down-regulating genes, such as FLS2-like and MKK5-like, putative orthologs of the Arabidopsis genes involved in pathogen perception and downstream signaling. CONCLUSIONS/SIGNIFICANCE: We have identified specific bean genes and uncovered metabolic processes and pathways that may be involved in the immune response against pathogens. Our transcriptome database is a rich resource for mining novel defense-related genes, which enabled us to

  17. Colorado economic impact study on the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado state fiscal year 1993

    Energy Technology Data Exchange (ETDEWEB)

    1993-11-12

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year (FY) 1993. To capture employment benefits, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Rifle, and Gunnison, Colorado. An estimated 52 percent of the employees working on the UMTRA Project responded to this information request. Economic data were requested from each prime subcontractor, as well as from the Remedial Action Contractor. The most significant benefits associated with the UMTRA Project in Colorado are: Direct employment was estimated at 894 workers; An estimated 89 percent of all direct employment was local; Secondary employment resulting from remedial action at the active Colorado UMTRA Project sites and the Grand Junction vicinity property program is estimated at 546 workers. Total employment (direct and secondary) is estimated at 1440 workers for the period of study (July 1, 1992, to June 30, 1993). An estimated $24.1 million was paid in wages to UMTRA workers in Colorado during FY1993; Direct and secondary wage earnings were estimated at $39.9 million; Income tax payments to the state of Colorado were estimated at $843,400 during FY1993; The gross economic impact of UMTRA Project activities in the state of Colorado is estimated at $70 million during the 1-year study period; and the net economic benefit to the state of Colorado was estimated at $57.5 million, or $5.90 per dollar of funding provided by Colorado. This figure includes both direct and secondary benefits but does not include the impact of alternative uses of the state funding.

  18. 78 FR 52600 - Colorado Disaster # CO-00054

    Science.gov (United States)

    2013-08-23

    ... ADMINISTRATION Colorado Disaster CO-00054 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Colorado dated 08/14/2013. Incident: Black Forest Fire. Incident Period: 06/11/2013 through 06/21/2013. Effective Date:...

  19. 75 FR 60151 - Colorado Disaster # CO-00033

    Science.gov (United States)

    2010-09-29

    ... ADMINISTRATION Colorado Disaster CO-00033 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of COLORADO dated 09/23/2010. Incident: Fourmile Canyon Fire. Incident Period: 09/06/2010 through 09/18/2010. Effective...

  20. Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris

    Science.gov (United States)

    The common bean (Phaseolus vulgaris) is the most important food legume in the world, but its production is severely limited by several biotic and abiotic stressors. In search of a sustainable solution to this problem, we conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11...

  1. Identifying genes involved in nodulation and symbiotic nitrogen fixation (SNF) in Phaseolus vulgaris using RNAseq technology

    Science.gov (United States)

    Phaseolus vulgaris (common bean) is one of the most important grain legumes for direct human consumption. It comprises 50% of the grain legumes consumed worldwide and is important as a primary source of dietary protein in developing countries. Legumes form a unique symbiotic relationship with rhizob...

  2. Virulence of Macrophomina phaseolina isolates in common bean (Phaseolus vulgaris) genotypes

    Science.gov (United States)

    Charcoal rot caused by Macrophomina phaseolina (Tassi) Goid, is an important disease in common beans (Phaseolus vulgaris) in the dry and warmer areas of Puerto Rico and in much of the tropics and subtropics worldwide. The virulence of three isolates from Isabela (Mph-ISA-TARS), Juana Diaz (Mph-JD) a...

  3. [Glycemic response to consumption of a cereals and legume (Phaseolus vulgaris) bar on healthy individuals].

    Science.gov (United States)

    Zambrano, Rosaura; Granito, Marisela; Valero, Yolmar

    2013-06-01

    The objective of this work was to formulate a cereals and legume (Phaseolus vulgaris) bar and assess its impact on the glycemic response of healthy individuals, in order to contribute to the healthy food supply beneficial to consumers. A mixture of cereals (corn and oats) and different percentages (20 and 30%) of Phaseolus vulgaris was used to formulate the bar. Additionally, a legume cereal bar without legumes (bar control) was prepared. The bar with 30% of Phaseolus vulgaris was selected through sensory evaluation, being scored with better flavor and texture. This combination of cereals and legumes aminoacid improves complementation and reaches the formulation criteria previously established. Chemical characterization indicated a higher protein content in the bar with 30% of Phaseolus vulgaris (13.55%) relative to the bar control (8.5%). The contents of fat, ash and dietary fiber did not differ between the two bars evaluated. However, the soluble fiber and resistant starch of the selected bar was a 32.05% and 18.67%, respectively, than in the control bar; this may contribute to decreasing the rate of glucose uptake. The selected bar presented a low glycemic index (49) and intermediate glycemic load (12.0) in healthy volunteers, which could lead to a possible reduction in the rate of absorption of glucose into the bloodstream, associated with a carbohydrate content of slow absorption. This bar represents a proposal of a healthy snack for the consumer.

  4. THE ACTION OF UV RADIATION ON MITOTIC INDEX AND MITOTIC DIVISION PHASES AT PHASEOLUS VULGARIS L

    Directory of Open Access Journals (Sweden)

    Csilla Iuliana Bara

    2005-08-01

    Full Text Available In this work, damaging effects of UV radiations on bean Phaseolus vulgaris L. plantule root tips were investigated. Our study proves that by bean plants, the decrease of cell division frequency appears to be part of protection mechanism against especially the short waved UV radiation, with variations depending on cultivar.

  5. Proteomic analysis of common bean (Phaseolus vulgaris) by two-dimensional gel electrophoresis and mass spectrometry

    Science.gov (United States)

    The modern cultivated common bean (Phaseolus vulgaris) has evolved from wild common beans distributed in Central America, Mexico and the Andean region of South America. It has been reported that wild common bean accessions have higher levels of protein content than the domesticated dry bean cultiva...

  6. Rhizosphere microorganisms affected by soil solarization and cover cropping in Capsicum annuum and Phaseolus lunatus agroecosystems

    Science.gov (United States)

    Field experiments were conducted to evaluate the effects of soil solarization or cover cropping on bell pepper (Capsicum annuum) and lima bean (Phaseolus lunatus, L.) rhizosphere microorganisms. In Experiment I, flat surface solarization (FSS), raised bed solarization (RBS), cowpea (Vigna unguiculat...

  7. Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Martin, Kathleen; Singh, Jugpreet; Hill, John H; Whitham, Steven A; Cannon, Steven B

    2016-08-11

    Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in Phaseolus to BCMV infection have not yet been well characterized. We report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar 'Stringless green refugee') to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns. We identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense.

  8. Formation of adventitious roots on green leaf cuttings of Phaseolus vulgaris L.

    NARCIS (Netherlands)

    Oppenoorth, Johanna Margriet

    1980-01-01

    n this thesis the development of adventitious roots on green leaf cuttings of Phaseolus vulgaris L. is studies. The use of green leaf cuttings has the advantage that the leaf blade provides the developing roots inthe petiole with all the nutrients required, a disadvantage is that the composition of

  9. Bioacoustics of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) on Phaseolus vulgaris (Fabaceae)

    Science.gov (United States)

    Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae), is an economically important pest of common bean Phaseolus vulgaris L. (Fabaceae) in the tropics and subtropics. It is difficult to detect the presence of A. obtectus because the larvae are cryptic and spend most of their developmental time...

  10. Demonstrating a nutritional advantage to the fast cooking dry bean (Phaseolus vulgaris L.)

    Science.gov (United States)

    Dry beans (Phaseolus vulgaris L.) are a nutrient dense food rich in protein and micronutrients. Despite their nutritional benefits, long cooking times limit the consumption of dry beans worldwide, especially in nations where fuelwood for cooking is often expensive or scarce. This study evaluated the...

  11. Transcriptome of common bean (Phaseolus vulgaris) through RNA-seq: nodulation, symbiotic nitrogen fixation, transcription factors

    Science.gov (United States)

    Phaseolus vulgaris (common bean) is one of the most important grain legumes for direct human consumption. It comprises 50% of the grain legumes consumed worldwide and is important as a primary source of dietary protein in developing countries. We have performed next generation sequencing (RNA-seq) o...

  12. Phenotypic variation in a core collection of common bean (Phaseolus vulgaris L.) in the Netherlands

    NARCIS (Netherlands)

    Zeven, A.C.; Waninge, J.; Hintum, van Th.J.L.; Singh, S.P.

    1999-01-01

    Forty accessions, forming a core collection of mainly bush type of the common bean (Phaseolus vulgaris L.) germplasm in the Netherlands, were evaluated for 14 qualitative and quantitative traits at the Agricultural University, Wageningen (WAU), the Netherlands in 1992. These and an additional 117 Du

  13. Selenium impacts on razorback sucker, Colorado River, Colorado: II. Eggs

    Science.gov (United States)

    Hamilton, S.J.; Holley, K.M.; Buhl, K.J.; Bullard, F.A.

    2005-01-01

    Effects on hatching and development of fertilized eggs in adult razorback sucker (Xyrauchen texanus) exposed to selenium in flooded bottomland sites near Grand Junction, Colorado, were determined. After 9 months exposure, fish were collected and induced to spawn and eggs collected for inorganic element analyses. A 9-day egg study was conducted with five spawns from Horsethief ponds, six spawns from Adobe Creek channel, and four spawns from North Pond using a reference water and site waters. Selenium concentrations in eggs were 6.5 ??g/g from Horsethief, 46 ??g/g from Adobe Creek, 38 ??g/g from North Pond, and 6.0 ??g/g from brood stock. Eggs from young adults had a smaller diameter and higher moisture content than brood stock. There were no differences among the four sources in viability, survival, hatch, hatchability, or mortality of deformed embryos or larvae. Adobe Creek larvae had more deformed embryos in eggs held in site water than held in reference water. There were significant negative correlations between selenium concentrations in adult muscle plugs and percent hatch, egg diameter, and deformities in embryos. Results from this study suggest that selenium contamination in parts of the upper basin of the Colorado River should be a major concern to recovery efforts for endangered fish.

  14. Selenium impacts on razorback sucker, Colorado River, Colorado II. Eggs.

    Science.gov (United States)

    Hamilton, Steven J; Holley, Kathy M; Buhl, Kevin J; Bullard, Fern A

    2005-05-01

    Effects on hatching and development of fertilized eggs in adult razorback sucker (Xyrauchen texanus) exposed to selenium in flooded bottomland sites near Grand Junction, Colorado, were determined. After 9 months exposure, fish were collected and induced to spawn and eggs collected for inorganic element analyses. A 9-day egg study was conducted with five spawns from Horsethief ponds, six spawns from Adobe Creek channel, and four spawns from North Pond using a reference water and site waters. Selenium concentrations in eggs were 6.5 microg/g from Horsethief, 46 microg/g from Adobe Creek, 38 microg/g from North Pond, and 6.0 microg/g from brood stock. Eggs from young adults had a smaller diameter and higher moisture content than brood stock. There were no differences among the four sources in viability, survival, hatch, hatchability, or mortality of deformed embryos or larvae. Adobe Creek larvae had more deformed embryos in eggs held in site water than held in reference water. There were significant negative correlations between selenium concentrations in adult muscle plugs and percent hatch, egg diameter, and deformities in embryos. Results from this study suggest that selenium contamination in parts of the upper basin of the Colorado River should be a major concern to recovery efforts for endangered fish.

  15. 7 CFR 948.151 - Colorado Potato Committee membership.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Colorado Potato Committee membership. 948.151 Section... POTATOES GROWN IN COLORADO Rules and Regulations Modification of Inspection Requirements § 948.151 Colorado Potato Committee membership. The Colorado Potato Committee shall be comprised of six members...

  16. Energy Smart Colorado, Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Gitchell, John M. [Program Administrator; Palmer, Adam L. [Program Manager

    2014-03-31

    Energy Smart Colorado is an energy efficiency program established in 2011 in the central mountain region of Colorado. The program was funded through a grant of $4.9 million, awarded in August 2010 by the U.S. Department of Energy’s Better Buildings Program. As primary grant recipient, Eagle County coordinated program activities, managed the budget, and reported results. Eagle County staff worked closely with local community education and outreach partner Eagle Valley Alliance for Sustainability (now Walking Mountains Science Center) to engage residents in the program. Sub-recipients Pitkin County and Gunnison County assigned local implementation of the program in their regions to their respective community efficiency organizations, Community Office for Resource Efficiency (CORE) in Pitkin County, and Office for Resource Efficiency (ORE) in Gunnison County. Utility partners contributed $166,600 to support Home Energy Assessments for their customers. Program staff opened Energy Resource Centers, engaged a network of qualified contractors, developed a work-flow, an enrollment website, a loan program, and a data management system to track results.

  17. Estabilidad de rendimiento en genotipos mesoamericanos de frijol de grano en México Yield stability of improved mesoamerican genotypes of black common bean in Mexico

    Directory of Open Access Journals (Sweden)

    Ernesto López Salinas

    2011-02-01

    Full Text Available En México se consumen más de 400 000 t de frijol de grano negro y la producción nacional es insuficiente por lo que se recurre a la importación. Con la siembra de variedades de mayor rendimiento potencial que las de uso actual y amplia adaptación, es factible obtener una mayor producción y disminuir o evitar las importaciones. El objetivo fue determinar el rendimiento y adaptabilidad de líneas y variedades de frijol negro, raza mesoamericana, en diferentes ambientes de México (uno en Chiapas, tres en Veracruz, uno en Puebla, uno en el Estado de México, dos en Guanajuato, uno en Tamaulipas, dos en Durango, uno en Zacatecas, uno en Nayarit y dos en Sinaloa en 2008. El experimento se estableció en diseño látice 4*4 con cuatro repeticiones, en parcelas de cuatro surcos de 5 m de longitud, separados 0.76 m. Durante la conducción de los ensayos se determinaron diversas características agronómicas, entre ellas el rendimiento. Con los datos de rendimiento se realizó análisis combinado (ambientes-genotipos y se estimaron los parámetros de estabilidad, con el modelo de efectos principales aditivos e interacción multiplicativa. Se detectaron diferencias altamente significativas (pIn Mexico, over 400 000 t of black beans are consumed every year, and the country's production is insufficient, which is why it is also imported. By planting varieties with a higher potential yield tan those used nowadays, greater amounts can be produced, and imports reduced or even avoided. The aim was to establish the yield and adaptability of lines and varieties of Mesoamerican breed of black beans, in different environments in Mexico (one in Chiapas, three in Veracruz, one in Puebla, one in the State of Mexico, two in Guanajuato, one in Tamaulipas, two in Durango, one in Zacatecas, one in Nayarit and two in Sinaloa in 2008. The experiment was set up in a 4*4 lattice design with four repetitions, in fields of four five-meter long furrows, separated by 0.76 m

  18. Pinto Coloso, nueva variedad de frijol para el estado de Durango Pinto Coloso, a new dry bean variety for the State of Durango

    Directory of Open Access Journals (Sweden)

    Rigoberto Rosales Serna

    2010-12-01

    Full Text Available En Durango los productores agrícolas demandan variedades de frijol pinto con grano tolerante al oscurecimiento acelerado de la testa y tamaño mayor en relación con Pinto Saltillo, la cual es la variedad más popular actualmente. El objetivo fue generar variedades superiores a Pinto Saltillo en calidad comercial, tolerancia similar al oscurecimiento de la testa y mayor tamaño de grano. En el INIFAP-Durango se generó la variedad Pinto Coloso (PT08036, con el método genealógico, a partir de la cruza entre Pinto Mestizo y Pinto Saltillo [(Pinto Mestizo/Pinto Saltillo-69-2-2]. Esta variedad mostró rendimiento promedio de 1 715 kg ha-1 y fluctuaciones entre 923 kg ha-1 hasta 2 645 kg ha-1. En promedio Pinto Coloso, comparado con Pinto Saltillo, presentó mayor precocidad a floración (40 vs 45 días, madurez (81 vs 88 días y tamaño del grano (39 vs 30. El crecimiento de Pinto Coloso es de enredadera indeterminada, con guías cortas no trepadoras, la altura del dosel oscila entre 34 y 38 cm y la guía crece 75 cm. Pinto Coloso tiene granos de 48 g por 100 semillas y un intervalo de peso entre 30 y 48 g por cada 100 semillas. El grano es transversalmente elíptico, de forma externa elíptica-romboidal, color crema de la testa, pintas de color café y el hilio es amarillo-naranja. Pinto Coloso mostró tolerancia a la antracnosis y roya; así como valores medios y altos de susceptibilidad a tizón común y pudriciones de raíz. Esta variedad está en proceso de validación en terrenos de productores para establecer su adaptabilidad y potencial para incrementar la calidad del frijol pinto producido en México.In Durango, farmers demand pinto bean cultivars with slow darkening grains and larger seed size compared to Pinto Saltillo, which is currently the most popular variety. The aim was to generate commercially superior varieties showing slow darkening coat, earlier maturity and larger seed size in comparison to Pinto Saltillo. Pinto Coloso (PT

  19. Adaptación, resistencia múltiple a enfermedades y tolerancia a suelos ácidos en genotipos de frijol

    Directory of Open Access Journals (Sweden)

    Ernesto L\\u00F3pez

    2003-01-01

    Full Text Available El objetivo del presente trabajo fue seleccionar genotipos de frijol con alto potencial de rendimiento, amplia adaptación, resistencia múltiple a enfermedades y tolerancia a suelos ácidos. Para lo cual, durante el ciclo otoño-invierno 1999-2000, se estableció un experimento uniforme de rendimiento de 16 genotipos incluyendo al testigo Negro Jamapa en cuatro ambientes en Veracruz y Chiapas, México. Se utilizó el diseño experimental de bloques completos al azar con tres repeticiones. Se evaluó el rendimiento de grano mediante análisis de varianza individual, combinado y parámetros de estabilidad. Los datos de reacción a enfermedades se cuantificaron con una escala de 1- 9. Para evaluar la tolerancia a suelos ácidos se utilizó el Índice de Eficiencia (IE. Los resultados de rendimiento de grano y reacción a enfermedades, señalaron diferencias significativas para localidades, genotipos y la interacción localidad-genotipo; mientras que para suelos ácidos no hubo significancia entre localidades y la interacción. Los mejores genotipos en cuanto a rendimiento, adaptación y tolerancia a suelos ácidos fueron Negro 8025, DOR-454, Icta Ju 95-112, Icta Ju 93-15 y DOR-448, de los cuales el primero y el último presentaron resistencia múltiple a enfermedades. Las líneas UCR-55, TLP- 22 y DOR-500, mostraron también buenos rendimientos y resistencia múltiple a enfermedades.

  20. Prácticas de conservación de suelos en sistemas de cultivo maíz-frijol en Cuba

    Directory of Open Access Journals (Sweden)

    Alfonso Claro A.

    2000-01-01

    Full Text Available Se realizaron dos ensayos en segmentos de microcuencas en condiciones de producción de la Provincia de Pinar del Río: uno en Campo Hermoso y el otro en La Comunidad La Leña, Cuba. En ambas localidades se rotó el frijol variedad Bat 304 y Tomeguin 93, seguidos de maíz asociado con Mucuna deeringianum y se establecieron bordes de desagüe protegidos con barreras vivas de Leucaena leucocephala y Gliricidia sepium. Las pérdidas de suelos se estimaron en piscinas de sedimentación y limnígrafos Valday en Campo Hermoso y en el caso de La Leña sólo se determinaron las variaciones de la pendiente y el espesor máximo de sedimentos (EMS al final de cada ciclo. El suelo fue un Ferralítico Cuarcítico (Ultisol, la fertilización se realizó de acuerdo a sus reservas, la preparación y labores culturales con tracción animal. Los resultados al cabo de cinco años mostraron una reducción en las pérdidas de suelos por debajo del umbral permisible (5 t/ha, aumento de la altura de las terrazas debido a los sedimentos acumulados, mayor disponibilidad de agua en la sección receptora, mayor cobertura en el período lluvioso, disminución del efecto erosivo de la lluvia, reciclaje de nutrimentos a través de los abonos verdes e incremento de los rendimientos respecto a las áreas donde no se realizaron prácticas de manejo. Se concluyó que el manejo integrado de los diferentes factores en laderas con pendientes mayores de 5%, es necesario para la conservación de suelos y agua

  1. Adaptación del frijol a suelos de baja fertilidad bajo presión de enfermedades foliares en Costa Rica

    Directory of Open Access Journals (Sweden)

    Rodolfo Araya

    2000-01-01

    Full Text Available El objetivo de este estudio fue identificar germoplasma y líneas mejoradas de alta productividad, en suelos de baja fertilidad y bajo presión de antracnosis y mancha angular. Durante 1996 y 1997 se evaluaron fuentes de tolerancia a la baja fertilidad de diversa procedencia geográfica y con resistencia para otros factores bióticos y abióticos. Se caracterizaron 562 genotipos, 251 mejorados y 311 criollos, en dos localidades de Costa Rica: Fraijanes (1750 msnm; 15,3 °C; 3025 mm; 10º09’ Latitud norte y 84°12’ Longitud oeste y Alajuela (840 msnm, 23,1 °C; 1966 mm; 10°01’ Latitud norte y 84° 16’ Longitud oeste. La densidad de siembra fue de 15 semillas por metro lineal y el tamaño de parcela varió con base en la disponibilidad de semilla; a la siembra sólo se adicionó inoculante de Rhizobium tropici al surco de siembra (4 kg/ha. La línea A321, el testigo local UCR 55, A483, G11640; G19696, G10843 y BAC50 mostraron alto rendimiento en las dos localidades. Los genotipos de alto rendimiento en una localidad fueron: en Alajuela: Guanajuato 132, Negro INIFAP, VAX 2, MAR 3 y en Fraijanes G13860, G92, MOC112, Bayo Alteño, J 117; Negro 8025 y A 247. Se identificó germoplasma no mejorado de diferente acervo genético, procedente de México, Perú y Guatemala, con tolerancia a suelos de baja fertilidad. Esto indica la importancia de evaluar material criollo y del intercambio de germoplasma entre países. Los genotipos superiores serán utilizados en el mejoramiento del frijol común para agricultura de bajo uso de insumos

  2. USGS Colorado Water Science Center bookmark

    Science.gov (United States)

    ,

    2016-12-05

    The U.S. Geological Survey Colorado Water Science Center conducts its water-resources activities primarily in Colorado in cooperation with more than 125 different entities. These activities include extensive data-collection efforts and studies of streamflow, water quality, and groundwater to address many specific issues of concern to Colorado water-management entities and citizens. The collected data are provided in the National Water Information System, and study results are documented in reports and information served on the Internet.

  3. Colorado geology then and now: following the route of the Colorado Scientific Society's 1901 trip through central Colorado

    Science.gov (United States)

    Simmons, Beth

    2013-01-01

    In 1901, Charles Van Hise asked Samuel Emmons and Whitman Cross to organize a grand excursion across Colorado as part of the combined meeting of the American Association for the Advancement of Science, GSA, and the Colorado Scientific Society (CSS). This trip replays part of that 10-day excursion across Colorado. Shortened to three days, this trip takes in some of the same sites as the 1901 trip, plus adds others of interest along the route where CSS members are reinventing geological interpretations. The trip will follow the precedent set in 1901; CSS members will serve as “site or stop hosts” in addition to the trip leader and drivers. While walking in the steps of the most famous of our profession we will also see some of the most magnificent scenery of Colorado.

  4. Professional Orientation of Colorado PR Practitioners

    Science.gov (United States)

    Lattimore, Dan L.; And Others

    1974-01-01

    Findings indicate that a majority of public relations practitioners are highly educated, have professional media backgrounds as part of their professional experience, and are paid better than newspaper personnel in Colorado. (RB)

  5. Notes and comments on Colorado Refuges

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report is a summary of actual management actions, and plant community responses on Colorado refuges during 1992. It is part of the moist-soil expert system...

  6. Colorado River Mile System, Tenths of Miles

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This coverage contains points representing tenth of miles in the GCMRC river mile system. The points fall along the centerline of the Colorado River from Glen Canyon...

  7. Colorado Plateau Rapid Ecoregion Assessment Data Catalog

    Data.gov (United States)

    Bureau of Land Management, Department of the Interior — Datasets used in the analysis of the Colorado Plateau (COP) Rapid Ecoregion Assessment (REA).They can be downloaded via a layer package (lpk, similar to a zip file...

  8. A comparative study of phytohaemagglutinin and extract of Phaseolus vulgaris seeds by characterization and cytogenetics

    Science.gov (United States)

    Badari Nath, A. R. S.; Sivaramakrishna, A.; Marimuthu, K. M.; Saraswathy, Radha

    2015-01-01

    Phytohaemagglutinin (PHA) is a lectin obtained from Phaseolus vulgaris (red kidney beans), that acts as a mitogen in human leucocyte culture and is commercially available from Gibco®. This PHA (Gibco®) was found to be very expensive, hence other inexpensive sources that can be used in all kinds of cytogenetics labs (rich and poor), were attempted. One such successful attempt was PHA extract from seeds of P.vulgaris. This paper details the methodology of extraction and application of PHA from seeds of P.vulgaris. Attempts has been made to identify the chemical and physical properties of the products in the extract, analyzed by various spectroscopic and analytical techniques. The analysis clearly indicates that the product from Phaseolus seeds extract was found to be similar to the commercially available PHA (Gibco®) in the cytogenetic study of human leucocyte cultures. The present study enforces the possible utility of the plant extract directly for human leucocyte cultures.

  9. Description of Phaseolus vulgaris L. aborting embryos from ethyl methanesulfonate (EMS mutagenized plants

    Directory of Open Access Journals (Sweden)

    Silué, S.

    2013-01-01

    Full Text Available The aim of this study was to describe the embryos abortion process and the inheritance of the embryos abortion trait in Phaseolus vulgaris plants deficient in seed development. These plants were isolated within the second generation of an ethyl methanesulfonate (EMS TILLING population of P. vulgaris cv. 'BAT93'. Mutant embryos show abnormalities mainly in suspensors, shoot apical meristem (SAM and cotyledons from the globular to the cotyledon stages and abort before maturity compared to those observed in wild-type samples. Mutant embryos show also hyperhydricity and contain low amount of chlorophyll. Genetic analyses of F1, F2 and F3 populations from the crosses carried out between the mutagenized plants with aborting embryos and the wild-type plants indicated that the embryo abortion phenotype is maternally inherited and controlled by a single recessive gene. These Phaseolus mutant plants with aborting embryos constitute a valuable material for plant embryogenesis studies.

  10. Culinary and nutritional quality of Phaseolus vulgaris seeds as affected by environmental factors

    Directory of Open Access Journals (Sweden)

    Kigel J.

    1999-01-01

    Full Text Available Efficient selection for specific culinary and nutritional quality traits needs a better understanding of the genetic and environmental control of quality traits at the structural, physiological and biochemical levels. Field experiments indicate great variability in the Phaseolus gene pool regarding the content of antinutritional compounds, as well as in cooking characteristics of the seeds. These seed attributes are strongly affected by geographic location, edaphic and climatic conditions at site of cultivation. However, information on the influence of specific environmental factors (such as temperature, water availability, edaphic conditions, etc. on seed quality traits, as well as on their stability is very scarce. This lack of knowledge impairs a faster progress in the improvement of Phaseolus seed quality.

  11. A Phaseolus vulgaris Extract Reduces Cue-Induced Reinstatement of Chocolate Seeking in Rats

    OpenAIRE

    Lorrai, Irene; Piga, Valentina; Mauro A M Carai; Riva, Antonella; Morazzoni, Paolo; Gessa, Gian Luigi; Colombo, Giancarlo; Maccioni, Paola

    2016-01-01

    Previous evidence has suggested that treatment with a standardized dry extract of Phaseolus vulgaris reduced intake and operant self-administration of highly palatable foods and fluids in rats and mice. The present study was designed to assess whether such extract was also effective in reducing seeking behavior for a highly hedonic chocolate-flavored beverage, using a “reinstatement” procedure adopted from the drug addiction research field and modeling relapse behavior. Rats were initially tr...

  12. A PHASEOLUS VULGARIS EXTRACT REDUCES CUE-INDUCED REINSTATEMENT OF CHOCOLATE SEEKING IN RATS

    OpenAIRE

    Irene eLorrai; Valentina ePiga; Mauro Antonio Maria Carai; Antonella eRiva; Paolo eMorazzoni; Gian Luigi eGessa; Giancarlo eColombo; Paola eMaccioni

    2016-01-01

    Previous evidence has suggested that treatment with a standardized dry extract of Phaseolus vulgaris reduced intake and operant self-administration of highly palatable foods and fluids in rats and mice. The present study was designed to assess whether such extract was also effective in reducing seeking behavior for a highly hedonic chocolate-flavored beverage, using a reinstatement procedure adopted from the drug addiction research field and modeling relapse behavior. Rats were initially trai...

  13. PHYSIOLOGICAL CHANGES IN BEAN (PHASEOLUS VULGARIS L. LEAVES, INFECTED BY THE MOST IMPORTANT BEAN DISEA

    Directory of Open Access Journals (Sweden)

    MALGOJATA BEROVA

    2007-11-01

    Full Text Available Gas-exchange, plastid pigments and some other physiological parameters were determined in bean (Phaseolus vulgaris L. local populations leaves naturally infected by Xanthomonas campestris pv.phaseoli (Smith Dye, and Pseudomonas syringae pv.phaseolicola (Bukholder Young, Dye et Wilkie, and in healthy leaves (control. It was established that infected leaves had lower both plastid pigments content and photosynthetic activity as well as lower yield and quality of produce.

  14. Adhesion of Colletotrichum lindemuthianum Spores to Phaseolus vulgaris Hypocotyls and to Polystyrene

    OpenAIRE

    Young, David H.; Kauss, Heinrich

    1984-01-01

    Adhesion of Colletotrichum lindemuthianum spores to Phaseolus vulgaris hypocotyls and to polystyrene was inhibited by the respiratory inhibitors sodium azide and antimycin A, indicating a requirement for metabolic activity in adhesion. Various commercial proteins and Tween 80 also reduced adhesion to both surfaces. Binding was enhanced by the presence of salts: sodium, potassium, calcium, and magnesium chlorides were equally effective. The removal of surface wax from hypocotyls by chloroform ...

  15. Genetic diversity and structure of a worldwide collection of Phaseolus coccineus L.

    Science.gov (United States)

    Spataro, G; Tiranti, B; Arcaleni, P; Bellucci, E; Attene, G; Papa, R; Spagnoletti Zeuli, P; Negri, V

    2011-05-01

    Phaseolus coccineus L. is closely related to P. vulgaris and is the third most important cultivated Phaseolus species. Little is known about the patterns of its diversity. In this work, a representative collection of its worldwide diversity was initially developed. The collection includes 28 wild forms (WFs) and 52 landraces (LRs) from Mesoamerica (the crop domestication area), and 148 LRs from Europe (where the crop was introduced in the sixteenth century). The collection was studied by using 12 SSR molecular markers that were developed for the P. vulgaris genome. They were proved to be effective and reliable in P. coccineus in this work. Fourteen LRs of P. dumosus (previously identified as a subspecies of P. coccineus) were also studied. The genetic diversity, population structure and phylogenetic relationships were investigated. The results indicate that: (a) the European and Mesoamerican gene pools are clearly differentiated, (b) a certain reduction of diversity occurred with introduction into Europe, and (c) the Mesoamerican LRs (P. dumosus included) and WFs are closely related and are connected by a high gene flow. Inferences on the domestication process of P. coccineus are also presented. This study provides a picture of the genetic diversity distribution and outcomes with introduction into the Old World, which was not available before. It also underlines that the genetic diversity of both WFs and LRs is an important source for Phaseolus spp. breeding programs and deserves to be preserved in situ and ex situ.

  16. PÉRDIDAS CAUSADAS POR EL AMACHAMIENTO DEL FRIJOL (Aphelenchoides besseyi Christie Y REACCIÓN DEL GERMOPLASMA COMERCIAL AL PATÓGENO

    Directory of Open Access Journals (Sweden)

    N\\u00E9stor Felipe Chaves-Barrantes

    2012-01-01

    Full Text Available La determinación de pérdidas causadas por amachamiento (Aphelenchoides besseyi en campo se llevó a cabo entre 2007 y 2008, en las regiones Brunca y Huetar Norte de Costa Rica, y la prueba de reacción de variedades se realizó en 2009 en la región Brunca. Para determinar pérdidas se establecieron 83 microparcelas de 3 m a lo largo de la hilera del cultivo, en campos reproductores de semilla de la variedad Cabécar, donde se evaluó la incidencia de la enfermedad durante tres etapas fenológicas (V3 o V4, R6 y R8. Se cuantifi caron los principales indicadores de rendimiento para cada microparcela y, mediante un análisis de regresión y correlación, se estimaron las pérdidas causadas por el amachamiento. Para determinar la reacción de cultivares a la enfermedad, se expuso bajo presión de inóculo natural, diez plantas de cada variedad (Bribrí, Chánguena, Cabécar, Gibre, Chirripó, Guaymí y Brunca, y se comparó su producción de vainas con respecto a la misma cantidad de plantas sanas, por medio de una prueba de t para muestras independientes. Las pérdidas determinadas en condiciones de campo fueron del 71 al 80% en el número de vainas y del 70 al 85% en rendimiento. La reducción en el número de semillas por vaina (0-16% y en el peso de 100 semillas (0-19% fueron bajos. Ninguna de las variedades comerciales de frijol presentó resistencia al amachamiento, ya que se obtuvieron pérdidas superiores al 91% en el número de vainas en los cultivares evaluados, excepto Gibre (82%.

  17. Adaptación y rendimiento de la variedad de frijol “Negro Medellín” en el sureste de México

    Directory of Open Access Journals (Sweden)

    Ernesto L\\u00F3pez

    2000-01-01

    Full Text Available El presente estudio se llevó a cabo de 1994 a 1997 con el objetivo de evaluar la adaptación y rendimiento de la nueva variedad de frijol “Negro Medellín” en la región del Trópico Húmedo del sureste de México. Se condujeron diez ensayos de rendimiento, cinco en el estado de Veracruz, cuatro en Chiapas y uno en Guerrero. Los ensayos incluyeron 16 genotipos y se realizaron, de acuerdo a las facilidades disponibles en los sitios de prueba, bajo condiciones de humedad residual o de temporal. En las evaluaciones se utilizó un diseño de Bloques Completos al Azar con tres repeticiones. La variedad Negro Medellín resultó sobresaliente por su rendimiento, amplia adaptación, con respuesta superior en ambientes favorables y consistente (1226 kg/ha, bi >1,0, S2di = 0. Durante las evaluaciones, la variedad Negro Medellín resultó tolerante a las enfermedades: virus del mosaico dorado (BGMV, mancha angular (Phaoisariopsis griseola y roya (Uromyces appendiculatus var. appendiculatus, enfermedades de amplia distribución en la región tropical. Sin embargo, en una localidad de Veracruz Negro Medellín resultó susceptible a la antracnosis (Colletotrichum lindemuthianum. Además, la variedad Negro Medellín se validó en parcelas comerciales en campos de agricultores en diez localidades del sureste de México, localidades con clima tropical. En éstas parcelas comerciales, Negro Medellín resultó superior en rendimiento a diversos testigos, variedades criollas y mejoradas, de un 10 a 25%, obteniendo un rendimiento promedio de 1300 kg/ha. La variedad Negro Medellín está en trámite para su registro como nueva variedad para las regiones productoras del sureste de México

  18. Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. under different soil moisture levels near Nairobi, Kenya

    NARCIS (Netherlands)

    Muniafu, M.M.; Macharia, J.N.M.; Stigter, C.J.; Coulson, G.L.

    1999-01-01

    Leaf area development, dry weight accumulation and solar energy conversion efficiencies of Phaseolus vulgaris L. cv GLP-2 under two soil moisture levels in two contrasting seasons near Nairobi, Kenya were investigated. The experiment confirms that dry weights and yields of Phaseolus vulgaris are lim

  19. A fragment of chloroplast DNA was transferred horizontally, probably from non-eudicots, to mitochondrial genome of Phaseolus.

    Science.gov (United States)

    Woloszynska, Magdalena; Bocer, Tomasz; Mackiewicz, Pawel; Janska, Hanna

    2004-11-01

    The mitochondrial genomes of some Phaseolus species contain a fragment of chloroplast trnA gene intron, named pvs-trnA for its location within the Phaseolus vulgaris sterility sequence (pvs). The purpose of this study was to determine the type of transfer (intracellular or horizontal) that gave rise to pvs-trnA. Using a PCR approach we could not find the respective portion of the trnA gene as a part of pvs outside the Phaseolus genus. However, a BLAST search revealed longer fragments of trnA present in the mitochondrial genomes of some Citrus species, Helianthus annuus and Zea mays. Basing on the identity or near-identity between these mitochondrial sequences and their chloroplast counterparts we concluded that they had relocated from chloroplasts to mitochondria via recent, independent, intracellular DNA transfers. In contrast, pvs-trnA displayed a relatively higher sequence divergence when compared with its chloroplast counterpart from Phaseolus vulgaris. Alignment of pvs-trnA with corresponding trnA fragments from 35 plant species as well as phylogenetic analysis revealed that pvs-trnA grouped with non-eudicot sequences and was well separated from all Fabales sequences. In conclusion, we propose that pvs-trnA arose via horizontal transfer of a trnA intron fragment from chloroplast of a non-eudicot plant to Phaseolus mitochondria. This is the first example of horizontal transfer of a chloroplast sequence to the mitochondrial genome in higher plants.

  20. 77 FR 11524 - Town of Walden, Colorado; Notice of Application

    Science.gov (United States)

    2012-02-27

    ... Energy Regulatory Commission Town of Walden, Colorado; Notice of Application Take notice that on February 1, 2012, Town of Walden, Colorado (Walden) filed with the Federal Energy Regulatory Commission... area within which Walden may, without further Commission authorization, provide natural...

  1. Ecological Integrity Assessment for Colorado Wetlands, Field Manual

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A quick reviewed survey protocol framework developed by the Colorado Natural Heritage program on performing an Ecological Integrity Assessment (EIA) for Colorado...

  2. Soil Management and Efficiency of Rhizobia Strains of Cowpea Vigna unguiculata (L. Walp. in the Tropics Manejo de Suelo y Eficiencia de Cepas de Rizobio de Frijol Vigna unguiculata (L. Walp. en los Trópicos

    Directory of Open Access Journals (Sweden)

    José Geraldo Donizetti dos Santos

    2011-12-01

    Full Text Available In the humid tropics, the largest obstacle to the implementation of sustainable farming systems is the reduced efficiency of nutrient use by crops. This study assesses the effectiveness of five selected rhizobia strains in symbiosis with cowpea (Vigna unguiculata (L. Walp., with the objective of replacing N fertilization in the predominant agricultural system used by smallholder farmers. The study was carried out in three adjacent areas with distinct agricultural uses: conventional tillage, itinerant agriculture, and a no-till system. The experimental design was in randomized complete blocks with four replicates and seven treatments: five rhizobia strains (BR3262, BR3267, BR3299, INPA3-11B, and UFLA 3-84 and two controls without inoculation (one without mineral N and another fertilized with 74 kg N ha-1. We measured the dry mass of 100 grains, nodules and shoots, as well as cowpea yields and calculated relative and absolute efficiency indices for dry biomass production of cowpea shoots. Agricultural uses affected the number and dry mass of the nodules and, consequently, the mass of the dry plant matter and bean yield. In terms of yield, there was a major difference between the conventional and the itinerant systems. Yield was around four times as high in the itinerant system (1009.9 kg ha-1 compared to 243.7 kg ha-1. Under conditions of cohesion-prone soils, the system of conventional tillage reduces the possibility of cultivation of a second harvest in the year even with inoculation or N fertilization.En los trópicos húmedos, el mayor obstáculo para la aplicación de sistemas de agricultura sostenible es la reducción de la eficiencia del uso de nutrientes por los cultivos. Este estudio evalúa la eficacia de cinco cepas de rizobios seleccionados en simbiosis con frijol (Vigna unguiculata (L. Walp., con el objetivo de sustituir la fertilización nitrogenada en el sistema de uso de suelo predominante utilizado por los pequeños agricultores

  3. Barriers to Enrollment in Health Coverage in Colorado

    OpenAIRE

    Martin, Laurie T.; Bharmal, Nazleen; Blanchard, Janice C.; Harvey, Melody; Williams, Malcolm

    2015-01-01

    As part of the implementation of the Affordable Care Act, Colorado has expanded Medicaid and also now operates its own health insurance exchange for individuals (called Connect for Health Colorado). As of early 2014, more than 300,000 Coloradans have newly enrolled in Medicaid or health insurance through Connect for Health Colorado, but there also continues to be a diverse mix of individuals in Colorado who remain eligible for but not enrolled in either private insurance or Medicaid. The Colo...

  4. 75 FR 52935 - Colorado Interstate Gas Company; Notice of Application

    Science.gov (United States)

    2010-08-30

    ... notice that on August 12, 2010, Colorado Interstate Gas Company (CIG), P.O. Box 1087, Colorado Springs... appurtenant facilities located in Douglas County, Colorado. Specifically, CIG states that it proposes: (1) To... adjacent to CIG's existing Spruce Hill Meter Station. CIG estimates the cost of the facilities will be $15...

  5. 76 FR 61382 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2011-10-04

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. ] SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  6. 75 FR 25877 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2010-05-10

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control ] Act of 1974...

  7. 78 FR 70574 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2013-11-26

    ....20350010.REG0000, RR04084000] Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  8. 77 FR 23508 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2012-04-19

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  9. 75 FR 27360 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2010-05-14

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  10. 75 FR 66389 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2010-10-28

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  11. 76 FR 24515 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2011-05-02

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of... Committee Act, the Bureau of Reclamation announces that the Colorado River Basin Salinity Control Advisory...) 524-3826; e-mail at: kjacobson@usbr.gov . SUPPLEMENTARY INFORMATION: The Colorado River Basin...

  12. 77 FR 61784 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2012-10-11

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974 (Pub....

  13. 78 FR 23784 - Colorado River Basin Salinity Control Advisory Council

    Science.gov (United States)

    2013-04-22

    ... Bureau of Reclamation Colorado River Basin Salinity Control Advisory Council AGENCY: Bureau of Reclamation, Interior. ACTION: Notice of public meeting. SUMMARY: The Colorado River Basin Salinity Control Advisory Council (Council) was established by the Colorado River Basin Salinity Control Act of 1974...

  14. 7 CFR 948.51 - Colorado Potato Committee.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Colorado Potato Committee. 948.51 Section 948.51... Order Regulating Handling Committees § 948.51 Colorado Potato Committee. The Colorado Potato Committee... selected from each area committee. Committeemen shall be selected by the Secretary from nominations of...

  15. Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus

    Directory of Open Access Journals (Sweden)

    Baudoin JP.

    1998-01-01

    Full Text Available Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzymes. Thirty-six haplotypes are identified on the basis of the polymorphism in fragment number and size. Three main phylogenetic groups, strongly supported through bootstrap analysis, are identified: (1 accessions from Phaseolus lunatus and Phaseolus xolocotzii; (2 accessions from Phaseolus glabellus; (3 accessions from Phaseolus vulgaris, Phaseolus polyanthus and Phaseolus coccineus. Within the third group, accessions of Phaseolus coccineus are scattered along the phylogenetic tree, which provides some evidence that coccineus accessions are paraphyletic with respect to Phaseolus vulgaris and Phaseolus polyanthus. An analysis of molecular variance applied on four species show that they are significantly differentiated with 79% of molecular variance among species and 21% within species. The results agree with previous investigations on chloroplast DNA variation in the genus Phaseolus, and suggest that PCRRFLP methods, which are technically less labour-intensive than previous methods, are of great value for phylogenetic investigations at the generic level.

  16. The instrumental climate history of southwestern Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Doesken, N.J.; McKee, T.B. [Colorado State Univ., Fort Collins, CO (United States)

    1995-09-01

    Instrumental observations of the climate of southwestern Colorado date back to about 1880. Climatic conditions since the late 19th century will be described with emphasis on temperatures, temperature ranges and observed precipitation. Typical seasonal patterns of temperature and precipitation will be shown, and variations and apparent trends over time will be discussed. Drought characteristics will be described based on a standardized precipitation index developed for Colorado. Finally, brief comments on the challenge of collecting accurate and consistent long-term data will be given.

  17. INDUCED CYTOMICTIC VARIATIONS AND SYNCYTE FORMATION DURING MICROSPOROGENESIS IN PHASEOLUS VULGARIS L.

    Science.gov (United States)

    Kumar, G; Chaudhary, N

    2016-01-01

    The intercellular translocation of chromatin material along with other cytoplasmic contents among the proximate meiocytes lying in close contact with each other commonly referred as cytomixis was reported during microsporogenesis in Phaseolus vulgaris L., a member of the family Fabaceae. The phenomenon of cytomixis was observed at three administered doses of gamma rays viz. 100, 200, 300 Gy respectively in the diploid plants of Phaseolus vulgaris L. The gamma rays irradiated plants showed the characteristic feature of inter-meiocyte chromatin/chromosomes transmigration through various means.such as channel formation, beak formation or by direct adhesion between the PMC's (Pollen mother cells). The present study also reports the first instance of syncyte formation induced via cytomictic transmigration in Phaseolus vulgaris L. Though the frequency of syncyteformation was rather low yet these could play a significant role in plant evolution. It is speculated that syncyte enhances the ploidy level of plants by forming 2n gametes and may lead to the production ofpolyploid plants. The phenomenon of cytomixis shows a gradual inclination along with the increasing treatment doses of gamma rays. The preponderance of cytomixis was more frequent during meiosis I as compared to meiosis II. An interesting feature noticed during the present study was the channel formation among the microspores and fusion among the tetrads due to cell wall dissolution. The impact of this phenomenon is also visible on the development of post-meiotic products. The formation of heterosized pollen grains; a deviation from the normal pollen grains has also been reported. The production of gametes with unbalanced chromosomes is of utmost importance and should be given more attention in future studies as they possess the capability of inducing variations at the genomic level and can be further utilized in the improvement of germplasm.

  18. The Colorado Lightning Mapping Array

    Science.gov (United States)

    Rison, W.; Krehbiel, P. R.; Thomas, R. J.; Rodeheffer, D.; Fuchs, B.

    2012-12-01

    A fifteen station Lightning Mapping Array (LMA) was installed in northern Colorado in the spring of 2012. While the driving force for the array was to produce 3-dimensional lightning data to support the Deep Convective Clouds and Chemistry (DC3) Experiment (Barth, this conference), data from the array are being used for several other projects. These include: electrification studies in conjunction with the CSU CHILL radar (Lang et al, this conference); observations of the parent lightning discharges of sprites (Lyons et al, this conference); trying to detect upward discharges triggered by wind turbines, characterizing conditions in which aircraft flying through clouds produce discharges which can be detected by the LMA, and other opportunities, such as observations of lightning in pyrocumulus clouds produced by the High Park Fire west of Fort Collins, CO. All the COLMA stations are solar-powered, and use broadband cellular modems for data communications. This makes the stations completely self-contained and autonomous, allowing a station to be installed anywhere a cellular signal is available. Because most of the stations were installed well away from anthropogenic noise sources, the COLMA is very sensitive. This is evidenced by the numerous plane tracks detected in its the vicinity. The diameter, D, of the COLMA is about 100 km, significantly larger than other LMAs. Because the error in the radial distance r is proportional to (r/D)2, and the error in the altitude z is proportional to (z/D)2, the larger array diameter greatly expands the usable range of the COLMA. The COLMA is able to detect and characterize lighting flashes to a distance of about 350 km from the array center. In addition to a web-based display (lightning.nmt.edu/colma), geo-referenced images are produced and updated at one-minute intervals. These geo-referenced images can be used to overlay the real-time lightning data on Google Earth and other mapping software. These displays were used by the DC3

  19. Visualization of resistance responses in Phaseolus vulgaris using reporter tagged clones of Bean common mosaic virus

    DEFF Research Database (Denmark)

    Naderpour, Masoud; Johansen, Ida Elisabeth

    2011-01-01

    Reporter tagged virus clones can provide detailed information on virus–host interactions. In Phaseolus vulgaris (bean), four recessive and one dominant gene are known to control infection by strains of the potyvirus species Bean common mosaic virus (BCMV). To study the interactions between BCMV...... breaking strains for further studies, BCMV RU1 was tagged with the sequence encoding green fluorescent protein (GFP), which was visualized directly without destruction of the tissue. In this paper we present details of the construction of the infectious clone and discuss its application in studies of BCMV...

  20. Effect of sulphite ions on the proline and polyamine content in the bean Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Piotr Karolewski

    2014-02-01

    Full Text Available The influence of sulphite (0.0125-0.2% on changes in the levels of free proline, protein bound proline and hydroxyproline and of free polyamines in the roots and leaves of two cultivars of beans (Phaseolus vulgaris differing in sensitivity to these ions was investigated. It was found that both cultivars reacted similarily, the response of the seedlings of the more sensitive cultivar occuring at lower concentrations of sulphite.The observed changes in the content of imino acids and polyamines are discussed.

  1. Analysis of the uptake of atmospheric ammonia by leaves of Phaseolus vulgaris L.

    Science.gov (United States)

    Van Hove, L. W. A.; Koops, A. J.; Adema, E. H.; Vredenberg, W. J.; Pieters, G. A.

    Individual leaves of Phaseolus vulgaris L. were exposed for 9 h in a leaf chamber to different NH 3 concentrations at different light intensities. The rates of NH 3-uptake, transpiration and photosynthesis were measured simultaneously. The flux density of NH 3 increased linearly with concentration in the range of 4-400μg m -3. Flux densities also increased with light intensity. Resistance analysis indicated that NH 3 transport into the leaf is via the stomata: transport via the cuticle is negligible under the experimental conditions. There is no internal resistance against NH 3 transport. The NH 3 flux was found not to influence the photosynthesis.

  2. Effects of copper on reserve mobilization in embryo of Phaseolus vulgaris L.

    Science.gov (United States)

    Karmous, Inès; Bellani, Lorenza M; Chaoui, Abdelilah; El Ferjani, Ezzedine; Muccifora, Simonetta

    2015-07-01

    The present research reports a biochemical and micro-submicroscopic analysis of copper effect on reserve mobilization during germination of Phaseolus vulgaris L. var. soisson nain hatif seeds. Dry embryonic cells are rich in protein bodies and little starch grains. In Cu-treated embryos copper inhibited 50% of albumin and globulin mobilization after 72 h imbibition. The severe alterations in treated embryo cells, observed by electron microscope, were probably the cause of the inability to utilize the amino acids freed by protein mobilization and so possibly the cause of the inhibition of P. vulgaris embryonic axis elongation.

  3. Reação de germoplasma crioulo de feijoeiro (Phaseolus vulgaris L.) a Colletotrichum lindemuthianum

    OpenAIRE

    Medeiros,Luis A.M.; Ricardo S. Balardin; Costa,Ivan F. D; Gulart,Caroline A.; Lenz,Giuvan

    2008-01-01

    Nos anos de 2003 e 2004, na Universidade Federal de Santa Maria, foram avaliados 60 genótipos de Feijoeiro Comum (Phaseolus vulgaris), de origem andina e meso-americana, com o objetivo de caracterizar sua estrutura genética quanto à resistência a 12 raças fisiológicas de Colletotrichum lindemuthianum, no estádio juvenil (V1/V2). As reações de cada genótipo foram avaliadas com base em uma escala de notas com valores de 1 a 9 a partir das quais foram calculados dois índices de resistência para ...

  4. Adhesion of Colletotrichum lindemuthianum Spores to Phaseolus vulgaris Hypocotyls and to Polystyrene.

    Science.gov (United States)

    Young, D H; Kauss, H

    1984-04-01

    Adhesion of Colletotrichum lindemuthianum spores to Phaseolus vulgaris hypocotyls and to polystyrene was inhibited by the respiratory inhibitors sodium azide and antimycin A, indicating a requirement for metabolic activity in adhesion. Various commercial proteins and Tween 80 also reduced adhesion to both surfaces. Binding was enhanced by the presence of salts: sodium, potassium, calcium, and magnesium chlorides were equally effective. The removal of surface wax from hypocotyls by chloroform treatment greatly reduced their subsequent ability to bind spores. The results suggest a similar mechanism for spore adhesion to the plant surface and to polystyrene, involving purely physical surface properties rather than group-specific binding sites.

  5. Curvas de absorción de nutrimentos en dos variedades, Bribri y sacapobres, de frijol común de grano rojo

    Directory of Open Access Journals (Sweden)

    Floria Bertsch

    2003-01-01

    Full Text Available Se generó las curvas de crecimiento y las de absorción de nutrimentos total y porcentual para 2 variedades de frijol de grano rojo, Bribri y Sacapobres, con el propósito de afinar las recomendaciones convencionales de fertilización, en cuanto a cantidad y momento de aplicación, que se practican en la actualidad en la zona frijolera del sur de Costa Rica. Se muestreó 5 plantas de cada variedad cada 15 días y fueron pesadas y analizadas por tipo de tejido (raíz, parte áerea, vaina. Las plantas crecieron en un suelo de buena fertilidad, con niveles moderados de P y Zn (8 y 2,3 mg l-1 en Olsen Modificado, respectivamente. Para ambas variedades los elementos consumidos en mayores cantidades fueron K y N, seguidos por Ca y luego por P y Mg. En el caso de la variedad Bribri, para una densidad de 156000 pl ha-1 y un rendimiento en peso seco de 3,5 t de vaina ha-1 (rendimiento comercial de grano de 1,2 t ha-1, las cantidades totales extraídas, en kg ha-1, correspondieron a: 156 de N, 24 de P, 222 de K, 117 de Ca, 26 de Mg, 1 de Fe, 0,3 de Cu, 0,7 de Zn y 0,6 de Mn. De estos valores, 37%, 52%, 39%, 11% y 32% de N, P, K, Ca y Mg, respectivamente, fueron extraídos directamente por la vaina. Para Sacapobres, con una densidad de 178500 pl ha-1 y un rendimiento de 2,9 t de vaina seca ha-1 (0,97 t ha-1 de rendimiento de grano comercial, la extracción total, en kg ha-1, fue de: 191 de N, 25 de P, 189 de K, 143 de Ca, 33 de Mg, 1,3 de Fe, 0,1 de Cu, 0,4 de Zn y 0,8 de Mn. Los porcentajes correspondientes a la cosecha fueron 33%, 40%, 34%, 7% y 19% para N, P, K, Ca y Mg, respectivamente. El nutrimento que más se recicla en los rastrojos es el Ca. En general, las dosis recomendadas en la región sur de Costa Rica (entre 3-5 sacos ha-1 de 50 kg de 10-30-10 llenan las necesidades de cosecha de estas variedades cuando los niveles de P y K en el suelo son adecuados. En cuanto a las etapas de máxima absorción de nutrimentos, para ambas variedades, m

  6. 78 FR 47815 - Colorado Disaster # CO-00060

    Science.gov (United States)

    2013-08-06

    ... ADMINISTRATION Colorado Disaster CO-00060 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the... Disaster Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite 6050, Washington,...

  7. Effectiveness of the AHEC Concept in Colorado.

    Science.gov (United States)

    Krugman, Richard D.; And Others

    1982-01-01

    Colorado's area health education program, the SEARCH program, designed to alleviate the maldistribution of health manpower, is described. It recruits new professionals to underserved areas through student/resident rotations and retains those professionals already there by providing accessible continuing education. (Author/MLW)

  8. Colorado's forest resources, 2002-2006

    Science.gov (United States)

    Michael T. Thompson; Joseph A. Duda; Larry T. DeBlander; John D. Shaw; Chris Witt; Todd A. Morgan; Michael C. Amacher

    2010-01-01

    This report presents a summary of the most recent inventory information for Colorado's forest lands. The report includes descriptive highlights and tables of area, number of trees, biomass, volume, growth, mortality, and removals. Most of the tables are organized by forest type, species, diameter class, or owner group. The report also describes inventory design,...

  9. Is Colorado's Voucher System Worth Vouching for?

    Science.gov (United States)

    Prescott, Brian T.

    2010-01-01

    In 2004 Colorado passed legislation enacting the nation's first voucher-based approach to financing higher education, known as the College Opportunity Fund (COF). The work of an unusual coalition that included higher education leaders, generally conservative policymakers, and others, COF completely replaced the traditional approach of subsidizing…

  10. Colorado Longitudinal Twin Study of Reading Disability

    Science.gov (United States)

    Wadsworth, Sally J.; DeFries, John C.; Olson, Richard K.; Willcutt, Erik G.

    2007-01-01

    The primary objectives of the present study are to introduce the Colorado Longitudinal Twin Study of Reading Disability, the first longitudinal twin study in which subjects have been specifically selected for having a history of reading difficulties, and to present some initial assessments of the stability of reading performance and cognitive…

  11. 76 FR 36039 - Colorado Regulatory Program

    Science.gov (United States)

    2011-06-21

    ... regulation of surface coal mining and reclamation operations on non- Federal and non-Indian lands within its... for the regulation of surface coal mining and reclamation operations in accordance with the... Office of Surface Mining Reclamation and Enforcement 30 CFR Part 906 Colorado Regulatory Program AGENCY...

  12. Besnoitiosis in rodents from Colorado. [Parasitic infestations

    Energy Technology Data Exchange (ETDEWEB)

    Dagle, G E; Winsor, T F; Adee, R R

    1976-01-01

    Parasitic cysts of Besnoitia jellisoni (coccidia) were found in rodents (Peromyscus maniculatus and Spermophilus tridecemlineatus) trapped in Eastern Colorado. The parasite was associated with a granulomatous inflammatory reaction in the lungs of each rodent and was disseminated in several organs from one Peromyscus. The ultrastructural appearance of the merozoites and the cyst wall formed by the host cell were studied.

  13. The presence of a below-ground neighbour alters within-plant seed size distribution in Phaseolus vulgaris

    NARCIS (Netherlands)

    Chen, B.; During, H.J.; Vermeulen, P.J.; Anten, N.P.R.

    2014-01-01

    * Background and Aims Considerable variation in seed size commonly exists within plants, and is believed to be favoured under natural selection. This study aims to examine the extent to which seed size distribution depends on the presence of competing neighbour plants. * Methods Phaseolus vulgaris p

  14. How weather during development of common bean (Phaseolus vulgaris L.) affects the crop's maximum attainable seed quality

    NARCIS (Netherlands)

    Muasya, R.M.; Lommen, W.J.M.; Muui, C.W.; Struik, P.C.

    2008-01-01

    NJAS wageningen journal of life sciences, Vol 56, No 1/2 (2008) Home About Log In Register Search Current Archives KLV Home > Vol 56, No 1/2 (2008) > Muasya Font Size: How weather during development of common bean (Phaseolus vulgaris L.) affects the crop¿s maximum attainable seed quality R.M.

  15. How weather during development of common bean (Phaseolus vulgaris L.) affects the crop's maximum attainable seed quality

    NARCIS (Netherlands)

    Muasya, R.M.; Lommen, W.J.M.; Muui, C.W.; Struik, P.C.

    2008-01-01

    NJAS wageningen journal of life sciences, Vol 56, No 1/2 (2008) Home About Log In Register Search Current Archives KLV Home > Vol 56, No 1/2 (2008) > Muasya Font Size: How weather during development of common bean (Phaseolus vulgaris L.) affects the crop¿s maximum attainable seed quality R.M.

  16. Mapping Fusarium solani and Aphanomyces euteiches root rot resistance and root architecture quantitative trait loci in common bean (Phaseolus vulgaris)

    Science.gov (United States)

    Root rot diseases of bean (Phaseolus vulgaris L.) are a constraint to dry and snap bean production. We developed the RR138 RIL mapping population from the cross of OSU5446, a susceptible line that meets current snap bean processing industry standards, and RR6950, a root rot resistant dry bean in th...

  17. THE EXPRESSION PROFILES OF SELECTED GENES IN DIFFERENT BEAN SPECIES (PHASEOLUS SPP. AS RESPONSE TO WATER DEFICIT

    Directory of Open Access Journals (Sweden)

    Tatjana KAVAR

    2012-01-01

    Full Text Available The aim of this study was to compare expression profiles of a number of transcripts from leaves of different Phaseolus species under drought stress, in order to ascertain whether changes in their expression in Phaseolus spp. are part of a general or a species specific response to drought. Relative gene expression analysis using quantitative PCR were carried out in P. coccineus, P. lunatus and P. acutifolius for 13 transcripts previously identified as up- or down-regulated in leaves of P. vulgaris. The mode of expression was found consistent within Phaseolus spp., despite the fact that the four species differ in their responses to drought at the physiological and morphological levels. The present results suggest that this is a common feature of the response of Phaseolus spp. The majority of the genes shown here to be influenced by water deficit in beans have been reported in other plant species under similar conditions, suggesting that they play a role in the general response to drought stress.

  18. Seed development in Phaseolus vulgaris L., Populus nigra L., and Ranunculus sceleratus L. with special reference to the microtubular cytoskeleton

    NARCIS (Netherlands)

    XuHan, X.

    1995-01-01

    In this thesis, seed development is investigated in celery-leafed buttercup ( Ranunculus sceleratus L.), bean ( Phaseolus vulgaris L.) and poplar ( Populus nigra L.). Developing embryos, endosperms and seed coats are investigate

  19. Isolates of Rhizoctonia solani can produce both web blight and root rot symptoms in common bean (Phaseolus vulgaris L.)

    Science.gov (United States)

    Rhizoctonia solani Kühn (Rs) is an important pathogen in the tropics, causing web blight (WB), and a widespread soil-borne root rot (RR) pathogen of common bean (Phaseolus vulgaris L.) worldwide. This pathogen is a species complex classified into 14 anastomosis groups (AG). Some AGs have been report...

  20. A non-destructive selection method for faster growth at suboptimal temperature in common bean (Phaseolus vulgaris)

    NARCIS (Netherlands)

    Drijfhout, E.; Oeveren, J.C. van; Jansen, R.C.

    1991-01-01

    A non-destructive method has been developed to select common bean (Phaseolus vulgaris L.) plants whose growth is less effected at a suboptimal temperature. Shoot weight was determined at a suboptimal (14°C) and optimal temperature (20°C), 38 days after sowing and accessions identified with a signifi

  1. Mapping snap bean (Phaseolus vulgaris L.) pod and color traits, in a dry bean x snap bean recombinant inbred population

    Science.gov (United States)

    Snap bean (Phaseolus vulgaris) breeding programs are tasked with developing varieties that meet the standards of the vegetable processing industry and ultimately that of the consumer; all the while matching or exceeding the field performance of existing varieties. While traditional breeding methods ...

  2. The interaction between endopolygalacturonase from Fusarium moniliforme and PGIP from Phaseolus Vulgaris studied by surface plasmon resonance and mass spectrometry

    DEFF Research Database (Denmark)

    Mattei, B; Cervone, F; Roepstorff, Peter

    2001-01-01

    Phaseolus vulgaris. PG hydrolyses the homogalacturonan of the plant cell wall and is considered an important pathogenicity factor of many fungi. PGIP is a specific inhibitor of fungal PGs and is thought to be involved in plant defence against phytopathogenic fungi. SPR was used either to study the effect...

  3. MicroRNAs Expression Profile in Common Bean (Phaseolus vulgaris) under Nutrient Deficiency Stresses and Manganese Toxicity

    Science.gov (United States)

    MicroRNAs (miRNAs) play a pivotal role in post-transcriptional regulation of gene expression in plants. The information on miRNAs in legumes is scarce. This work analyzes miRNAs in the agronomically important legume common bean (Phaseolus vulgaris. A hybridization approach of miRNAs-macroarrays prin...

  4. Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris Enzimas do metabolismo da sacarose em cultura celular de Bauhinia forficata, Curcuma zedoaria e Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Marcia Ometto de Mello

    2001-09-01

    Full Text Available The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.O objetivo deste trabalho foi estudar as enzimas do metabolismo da sacarose em culturas de célula em suspensão de Bauhinia forficata Link, Curcuma zedoaria Roscoe e Phaseolus vulgaris L. A via da invertase foi identificada nas três espécies estudadas. A via da sacarose sintase também foi responsável pelo metabolismo da sacarose em células de Curcuma zedoaria e Phaseolus vulgaris. Foram encontradas atividades maiores que 300 nmol min-1 mg-1 de proteína das enzimas invertase ácida e alcalina, UDPglicose pirofosforilase e fosfoglicomutase no extrato celular das três espécies de plantas. A sacarose sintase mostrou atividade baixa nas células de Bauhinia forficata. À medida que a concentração de sacarose no meio de cultura diminuiu, a atividade da sacarose sintase aumentou em células de Curcuma zedoaria e Phaseolus vulgaris. Ao final do período de cultura, quando os carboidratos se tornaram limitantes, as atividades das enzimas glicolíticas reduziram-se gradualmente.

  5. Los cultivos de frijol y maíz de grano bajo condiciones de secano en Zacatecas, México de 1980 a 2008 The crops of bean and maize under dry land conditions in the state of Zacatecas, Mexico from 1980 to 2008

    Directory of Open Access Journals (Sweden)

    Maximino Luna Flores

    2012-04-01

    Full Text Available Los cultivos de frijol y maíz de grano son los más importantes agrícola, social y económicamente en el estado de Zacatecas, desde hace muchos años. Los objetivos principales de este trabajo fueron: ver si la superficie sembrada, la siniestrada, la producción y el rendimiento unitario de frijol y maíz de grano cultivados bajo condiciones de secano del estado de Zacatecas han variado de 1980 a 2008; y evaluar que tanto afecta la precipitación a estas variables. Se usaron los datos que la SAGARPA pública a través de internet. Se obtuvieron las correlaciones y regresiones entre los datos de las variables de cada cultivo, para hacer un análisis más preciso. Se encontró que el volumen de precipitación registrado durante el ciclo de cultivo y su distribución, afectaron considerablemente la superficie sembrada, la siniestrada, el rendimiento unitario y el volumen de producción de grano de frijol y maíz de secano. La superficie siniestrada de frijol (15.2% fue inferior a la de maíz (18.6%; la de frijol aumentó 543 ha por cada milímetro de precipitación menos y la de maíz en 256 ha. El rendimiento medio de frijol de secano se incrementó 1.62% por año, y maíz 1.46%. La superficie sembrada de frijol disminuyó significativamente en los últimos años por efecto del programa de reconversión productiva, al aumentar principalmente las superficies de avena y maíz forrajeros.The bean and maize grain crops have been the most important, agriculturally, socially and economically in the State of Zacatecas, for many years. The main objectives of this work were to see if the surfaces planted and damaged, and if the production and the unit yields of beans and grain maize planted under dry land conditions in the State of Zacatecas, have varied from 1980 to 2008; and to evaluate how rainfall affects these variables. Data published by SAGARPA on the Internet was used. Correlations and regressions between the data for variables of each crop were

  6. Dynamics of a Novel Highly Repetitive CACTA Family in Common Bean (Phaseolus vulgaris).

    Science.gov (United States)

    Gao, Dongying; Zhao, Dongyan; Abernathy, Brian; Iwata-Otsubo, Aiko; Herrera-Estrella, Alfredo; Jiang, Ning; Jackson, Scott A

    2016-07-07

    Transposons are ubiquitous genomic components that play pivotal roles in plant gene and genome evolution. We analyzed two genome sequences of common bean (Phaseolus vulgaris) and identified a new CACTA transposon family named pvCACTA1. The family is extremely abundant, as more than 12,000 pvCACTA1 elements were found. To our knowledge, this is the most abundant CACTA family reported thus far. The computational and fluorescence in situ hybridization (FISH) analyses indicated that the pvCACTA1 elements were concentrated in terminal regions of chromosomes and frequently generated AT-rich 3 bp target site duplications (TSD, WWW, W is A or T). Comparative analysis of the common bean genomes from two domesticated genetic pools revealed that new insertions or excisions of pvCACTA1 elements occurred after the divergence of the two common beans, and some of the polymorphic elements likely resulted in variation in gene sequences. pvCACTA1 elements were detected in related species but not outside the Phaseolus genus. We calculated the molecular evolutionary rate of pvCACTA1 transposons using orthologous elements that indicated that most transposition events likely occurred before the divergence of the two gene pools. These results reveal unique features and evolution of this new transposon family in the common bean genome.

  7. Effects of sequential enzymatic hydrolysis on structural, bioactive and functional properties of Phaseolus lunatus protein isolate

    Directory of Open Access Journals (Sweden)

    Erik Polanco-Lugo

    2014-09-01

    Full Text Available Significant initiatives exist within the global food market to search for new, alternative protein sources with better technological, functional, and nutritional properties. Lima bean (Phaseolus lunatus L. protein isolate was hydrolyzed using a sequential pepsin-pancreatin enzymatic system. Hydrolysis was performed to produce limited (LH and extensive hydrolysate (EH, each with different degrees of hydrolysis (DH. The effects of hydrolysis were evaluated in vitro in both hydrolysates based on structural, functional and bioactive properties. Structural properties analyzed by electrophoretic profile indicated that LH showed residual structures very similar to protein isolate (PI, although composed of mixtures of polypeptides that increased hydrophobic surface and denaturation temperature. Functionality of LH was associated with amino acid composition and hydrophobic/hydrophilic balance, which increased solubility at values close to the isoelectric point. Foaming and emulsifying activity index values were also higher than those of PI. EH showed a structure composed of mixtures of polypeptides and peptides of low molecular weight, whose intrinsic hydrophobicity and amino acid profile values were associated with antioxidant capacity, as well as inhibiting angiotensin-converting enzyme. The results obtained indicated the potential of Phaseolus lunatus hydrolysates to be incorporated into foods to improve techno-functional properties and impart bioactive properties.

  8. Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans

    Directory of Open Access Journals (Sweden)

    Arishya Sharma

    2009-01-01

    Full Text Available A lectin has been isolated from seeds of the Phaseolus vulgaris cv. “Anasazi beans” using a procedure that involved affinity chromatography on Affi-gel blue gel, fast protein liquid chromatography (FPLC-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The lectin was comprised of two 30-kDa subunits with substantial N-terminal sequence similarity to other Phaseolus lectins. The hemagglutinating activity of the lectin was stable within the pH range of 1–14 and the temperature range of 0–80∘C. The lectin potently suppressed proliferation of MCF-7 (breast cancer cells with an IC50 of 1.3 μM, and inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 7.6 μM. The lectin evoked a mitogenic response from murine splenocytes as evidenced by an increase in [3H-methyl]-thymidine incorporation. The lectin had no antifungal activity. It did not stimulate nitric oxide production by murine peritoneal macrophages. Chemical modification results indicated that tryptophan was crucial for the hemagglutinating activity of the lectin.

  9. Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen.

    Science.gov (United States)

    Dall'Agnol, Rebeca Fuzinatto; Ribeiro, Renan Augusto; Ormeño-Orrillo, Ernesto; Rogel, Marco Antonio; Delamuta, Jakeline Renata Marçon; Andrade, Diva Souza; Martínez-Romero, Esperanza; Hungria, Mariangela

    2013-11-01

    Common bean (Phaseolus vulgaris L.) can establish symbiotic associations with several Rhizobium species; however, the effectiveness of most strains at fixing nitrogen under field conditions is very low. PRF 81(T) is a very effective strain, usually referred to as Rhizobium tropici and used successfully in thousands of doses of commercial inoculants for the common bean crop in Brazil; it has shown high rates of nitrogen fixation in all areas representative of the crop in the country. Here, we present results that indicate that PRF 81(T), although it belongs to the 'R. tropici group', which includes 10 Rhizobium species, R. tropici, R. leucaenae, R. lusitanum, R. multihospitium, R. miluonense, R. hainanense, R. calliandrae, R. mayense, R. jaguaris and R. rhizogenes, represents a novel species. Several morpho-physiological traits differentiated PRF 81(T) from related species. Differences were also confirmed in the analysis of rep-PCR (sharing less than 45 % similarity with the other species), MLSA with recA, atpD and rpoB genes, and DNA-DNA hybridization. The novel species, for which we propose the name Rhizobium freirei sp. nov., is able to establish effective root nodule symbioses with Phaseolus vulgaris, Leucaena leucocephala, Leucaena esculenta, Crotalaria juncea and Macroptilium atropurpureum. The type strain is PRF 81(T) ( = CNPSo 122(T) = SEMIA 4080(T) = IPR-Pv81(T) = WDCM 440(T)).

  10. Dynamics of a Novel Highly Repetitive CACTA Family in Common Bean (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Dongying Gao

    2016-07-01

    Full Text Available Transposons are ubiquitous genomic components that play pivotal roles in plant gene and genome evolution. We analyzed two genome sequences of common bean (Phaseolus vulgaris and identified a new CACTA transposon family named pvCACTA1. The family is extremely abundant, as more than 12,000 pvCACTA1 elements were found. To our knowledge, this is the most abundant CACTA family reported thus far. The computational and fluorescence in situ hybridization (FISH analyses indicated that the pvCACTA1 elements were concentrated in terminal regions of chromosomes and frequently generated AT-rich 3 bp target site duplications (TSD, WWW, W is A or T. Comparative analysis of the common bean genomes from two domesticated genetic pools revealed that new insertions or excisions of pvCACTA1 elements occurred after the divergence of the two common beans, and some of the polymorphic elements likely resulted in variation in gene sequences. pvCACTA1 elements were detected in related species but not outside the Phaseolus genus. We calculated the molecular evolutionary rate of pvCACTA1 transposons using orthologous elements that indicated that most transposition events likely occurred before the divergence of the two gene pools. These results reveal unique features and evolution of this new transposon family in the common bean genome.

  11. Comparative bioinformatic analysis of genes expressed in common bean (Phaseolus vulgaris L.) seedlings.

    Science.gov (United States)

    Melotto, Maeli; Monteiro-Vitorello, Claudia B; Bruschi, Adriano G; Camargo, Luis E A

    2005-06-01

    To rapidly and cost-effectively generate gene expression data, we developed an annotated unigene database of common bean (Phaseolus vulgaris L.). In this study, 3 cDNA libraries were constructed from the bean breeding line SEL1308, 1 from young leaf and 2 from seedlings inoculated or not inoculated with the fungal pathogen Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara, which causes anthracnose in common bean. To this date, 5255 single-pass sequences have been included in the database after selection based on sequence quality. These ESTs were trimmed and clustered using the computer programs Phred and CAP3 to form a unigene collection of 3126 unique sequences. Within clusters, 318 single nucleotide polymorphisms (SNPs) and 68 insertions-deletions (indels) were found, indicating the presence of paralogous gene families in our database. Each unigene sequence was analyzed for possible function using their similarity to known genes represented in the GenBank database and classified into 14 categories. Only 314 unigenes showed significant similarities to Phaseolus genomic sequences and P. vulgaris ESTs, which indicates that 90% (2818 unigenes) of our database represent newly discovered common bean genes. In addition, 12% (387 unigenes) were shown to be specific to common bean. This study represents a first step towards the discovery of novel genes in beans and a valuable source of molecular markers for expressed gene tagging and mapping.

  12. Rendimiento y reacción a enfermedades de genotipos de frijol en condiciones de temporal y humedad residual Yield and reaction to diseases of bean genotypes under rainfed conditions and residual moisture

    Directory of Open Access Journals (Sweden)

    Oscar Hugo Tosquy-Valle

    2012-07-01

    Full Text Available En Chiapas, Veracruz y Puebla, las enfermedades son uno de los principales factores que reducen el rendimiento de frijol. Los objetivos del trabajo fueron determinar el comportamiento productivo de genotipos de frijol negro en temporal y humedad residual y su reacción a enfermedades. En 2008 se estableció un ensayo en Ocozocoautla, Chiapas, Orizaba, Veracruz y Tecamachalco, Puebla, en temporal y en Medellín de Bravo y San Andrés Tuxtla, Veracruz, con humedad residual. Se evaluaron 11 variedades y cinco líneas, en diseño látice 4x4 con cuatro repeticiones. En campo se cuantifico su reacción al virus del mosaico común, mancha angular, roya y antracnosis, con la escala de 1 a 9 del CIAT, así como el rendimiento de grano en kilogramos por hectárea. Se realizó análisis de varianza de cada enfermedad, las cuales se correlacionaron con el rendimiento de grano. Así como análisis combinado del rendimiento de los genotipos por condición de humedad y análisis de conjunto de todos los ambientes de prueba. Para la separación de promedios se aplicó la DMS al 0.05. Negro Papaloapan rindió 1 753 kg ha-1 en temporal y 1 333 kg ha-1 con humedad residual; ambos rendimientos promedio fueron estadísticamente superiores al del resto de los genotipos. La incidencia de antracnosis (r=-0.516* en la primera condición y de mancha angular (r=-0.5 2 8 * en la segunda, disminuyeron significativamente el rendimiento de frijol. Negro Papaloapan fue el más productivo en ambas condiciones de humedad y mostró resistencia al mosaico común y mancha angular y tolerancia a antracnosis.In Chiapas, Veracruz and Puebla, diseases are one of the main factors that reduce the yield of beans. The objectives were to determine the productive performance ofblack bean genotypes in rainfed and residual moisture and, their reaction to disease. In 2008, a trial was made in Ocozocoautla, Chiapas, Orizaba, Veracruz and Tecamachalco, Puebla, in rainfed conditions and, in

  13. Influence of a natural-ingredient diet containing Phaseolus vulgaris on the colonization by segmented, filamentous bacteria of the small bowel of mice.

    Science.gov (United States)

    Klaasen, H L; Koopman, J P; van den Brink, M E; Bakker, M H; Beynen, A C

    1992-01-01

    The appearance of segmented, filamentous bacteria (SFBs) in the small bowel of mice is influenced by the composition of the diet, but the dietary components responsible are not known. The addition of ground, whole Phaseolus vulgaris to a natural-ingredient diet at the expense of part of the skim milk powder, ground barley and wheat middlings components, caused an increase of the colonization of the mouse small bowel by SFBs. This effect was not seen when whole Phaseolus was added to a purified diet at the expense of part of the casein, corn oil, coconut fat, corn starch, dextrose and cellulose components. In an attempt to identify the fraction of Phaseolus that might contain SFB-inducing substances, the skin and kernel fraction of the bean were added to the natural-ingredient diet. The skin and kernel fraction were found to be as effective in inducing SFB appearance as was whole Phaseolus.

  14. 75 FR 23288 - Notice of Public Meeting, Southwest Colorado Resource Advisory Council Meeting

    Science.gov (United States)

    2010-05-03

    ... will be held on June 4, 2010, in Dolores, Colorado; August 13, 2010, in Gunnison, Colorado; and October... 184, Dolores, Colorado 81323; August 13, 2010, at the Holiday Inn Express at 910 E. Tomichi,...

  15. Resistencia a sequía terminal en frijol negro tropical Comportamiento de variedades de maíz normal y con alta calidad de proteína para la región Golfo de México

    Directory of Open Access Journals (Sweden)

    Mark Frahm

    2003-01-01

    Full Text Available Con el objetivo de identificar cultivares de frijol negro tropical con resistencia a sequía y adaptación a la región Centroamericana, se diseñó un proyecto piloto colaborativo entre los programas de frijol de Zamorano, Honduras; Veracruz, México y la Universidad Estatal de Michigan (MSU; EE.UU, en el año 2002. Se desarrollaron dos poblaciones de líneas endog ámicas recombinantes (RILs derivadas de cruces entre la lí- nea resistente a sequía B98311 de MSU con TLP 19 y VAX 5, dos líneas mejoradas del CIAT con resistencia a enfermedades y adaptadas a las condiciones de América Central y México. Ambas poblaciones se evaluaron bajo riego y sequía terminal en Zamorano, Honduras y Veracruz, México. Los rendimientos se vieron reducidos por la sequía y por Macrophomina phaseolina, hongo causal de la pudrición carbonosa. El estrés por sequía, la presión de la enfermedad y los bajos rendimientos, contribuyeron a la obtención de altos coeficientes de variación, lo que hizo difícil la identificación y selecci ón de líneas superiores. La selección se basó en la posici ón de la media geométrica del rendimiento (MG calculada para cada línea con los datos de ambos tratamientos, riego y sequía. La línea L88-63 ocupó el primer lugar en la MG del rendimiento en ambas localidades. Evaluaciones posteriores conducidas en Honduras y Michigan, confirmaron el alto potencial de rendimiento y amplia adaptación de la línea L88- 63. El mejoramiento de la resistencia a la sequía en ambientes del trópico bajo, requiere la incorporación adicional de resistencia a M. phaseolina en las nuevas líneas consideradas para liberarse como nuevas variedades en esta región.

  16. Green pricing: A Colorado case study

    Energy Technology Data Exchange (ETDEWEB)

    Blank, E.; Udall, J.R.

    1996-12-31

    A model program for green pricing targeted primarily at large customers is proposed in this paper. The program would create a partnership between a local community group, a renewables advocacy group, and several Colorado utilities. The first part of the paper summarizes pertinent background issues, including utility experience with green pricing programs. The rest of the paper outlines the program proposal, focusing primarily on organizational structure.

  17. BOOKLET TO INSTITUTO PEDAGOGICO NACIONAL TEACHERS ABOUT SYMBIOSIS AND PROCESSES ON BIOTECHNOLOGY: THE BIOFERTILIZER Rhizobium sp IN Phaseolus vulgaris WITH ALTERNATIVE TO SYMBIOSIS FOR Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    María Camila Quevedo Rubiano

    2016-09-01

    Full Text Available This article presents the results of the thesis carried out in the research group of Biotechnology Teaching in Colombia, with the aim of providing teachers of Biology of Instituto Pedagogico Nacional a booklet that can strengthen the teaching of biotechnology processes using Rhizobium sp reduction of chemical fertilizers and symbiosis with Phaseolus vulgaris.   The booklet contains a proposal of practical activities that enable teachers of this institution to use spaces like the farm, enabling to teach biotechnology related to agronomy. Therefore, for this project was considered two Biological and Pedagogical approaches, the first is within the analytical empirical paradigm in the process of microbiological characterization of Rhizobium and their Biofertilizing ability in beans; and the teaching approach within the design of a booklet that includes the findings of this study as a contribution to the reduction of chemical fertilizers school farm. In order to have a complete analysis of the work it was subjected to quantitative and qualitative methods.   This biotech practice is included in the booklet showing in bioassays that bacteria has biofertilizer without inhibiting potential symbiosis, and that research and teaching biological concepts from scientific expertise can be promoted in Biology class for students to understand its context in a significant way, to be used in different levels of education; also it is a teaching strategy.

  18. Economic impact study of the Uranium Mill Tailings Remedial Action project in Colorado: Colorado state fiscal year 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-12-01

    This Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year (FY) 1995 (1 July 1994 through 30 June 1995). To capture employment information, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Gunnison, Maybell, Naturita, Rifle, and Slick Rock, Colorado. Economic data were requested from the Remedial Action Contractor (RAC), the Technical Assistance Contractor (TAC) and the US Department of Energy (DOE). The most significant benefits associated with the UMTRA Project in Colorado are summarized.

  19. Economic impact study of the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado state fiscal year 1993

    Energy Technology Data Exchange (ETDEWEB)

    1993-12-01

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year 1993 (July 1, 1992, through June 30, 1993). To capture employment benefits, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Rifle, and Gunnison, Colorado. An estimated 52 percent of the employees working on the UMTRA Project responded to this information request. Economic data were requested from each site prime subcontractor, as well as from the Remedial Action Contractor. The most significant benefits associated with the UMTRA Project in Colorado are summarized.

  20. Yield and drougth tolerance of six varieties of common bean (Phaseolus vulgaris L. under field condition

    Directory of Open Access Journals (Sweden)

    Yanitza Meriño Hernandez

    2015-01-01

    Full Text Available In two moisture conditions (drought and irrigation were evaluated six varieties of common bean (Phaseolus vulgaris L., with a factorial randomized complete blocks. The objectives of the study was to evaluate the effect caused by drought conditions crop varieties, identify high performance and features that enable them to adapt to varying conditions of soil moisture. With the data in yields between the two humidity conditions intensity indices of drought (IIS, susceptibility to drought (ISS, relative efficiency (IER, geometric mean (GM and percent yield losses were calculated . The results were statistically processed using the Statistica software version 8.0 for Windows, if significant differences Tukey test was applied to p<0.05. The selection based on levels ISS, MG, IER and PPR identified high yielding varieties adapted to drought and favorable moisture conditions.

  1. Mangifera indica and Phaseolus vulgaris in the bioindication of air pollution in Bahia, Brazil.

    Science.gov (United States)

    Lima, J S; Fernandes, E B; Fawcett, W N

    2000-07-01

    In this article are reported the results of a study on the concentration of ascorbic acid (AA) in Mangifera indica as passive monitor and in Phaseolus vulgaris as active monitor with the intention to study the effects of industrial emissions from the Petrochemical Complex of Camaçari (PCC), Bahia, Brazil, on the vegetation. Leaves from M. indica were collected in two sites in the region under direct influence of industrial emissions and in one presumed nonpolluted reference (background) site. Pots with P. vulgaris were exposed in the same sites. The AA increase in the leaves of M. indica from PCC sites indicates a stress situation. The small AA increase in the P. vulgaris exposed in the more polluted site indicates that the active monitor is a sensitive one. The decrease of its leaf area indicates the inability of this species to activate physiological protection mechanisms like an increase in AA production.

  2. Effect of lead on imbibition, germination, and growth of Phaseolus vulgaris L. and Zea mays L.

    Directory of Open Access Journals (Sweden)

    Gustavo Isaza Guzmán Isaza Guzmán

    2013-07-01

    Full Text Available Lead is highly reactive and it can be consequently toxic to living cells to both plants and humans. This heavy metal is a source of contamination to the environment and it disrupts natural cycles. The present study was aimed to evaluate the effect of lead on the imbibition process, germination and growth in the bean (Phaseolus vulgaris L. and maize (Zea mays L.. It was developed a system consisting of receptacles to expose flooded plants at different concentrations of the metal. Results showed that at concentrations of 5 g l-1 lead imbibition process was affected, but was more evident in bean. Germination percentage was not affected in maize seeds, while viability was affected in bean seeds. We observed statistically that there is an effect on organ growth of root, stem and leaf in both species in the presence of solution whose effect is most noticeable in bean plants. Key words: heavy metals,phytoremediation, stress, toxic substances

  3. Nitrogen assimilation by nodulate plants of Phaseolus vulgaris l. and Vigna unguiculata (l. ) walp

    Energy Technology Data Exchange (ETDEWEB)

    Neves, M.C.P.; Fernandes, M.S.; Sa, M.F.M. (Universidade Federal Rural do Rio de Janeiro (Brazil). Dept. de Solos)

    1982-05-01

    Under field conditions, the processes of nitrogen assimilation via nitrogenase and nitrate-reductase, the transport and the accumulation of nitrogen in nodulated plants of Phaseolus vulgaris cv. Rio Tibagi and Vigna unguiculata cv. Vita 34 were compared and contrasted. V. unguiculata showed better nodulation than P. vulgaris and consequently had higher rates of nitrogenase activity. The small nodulation of P. vulgaris resulted in greater dependence on soil mineral nitrogen as indicated by the higher rates of nitrate-reductase acitivty compared with V. unguiculata, especially during reproductive stage of growth. The superiority of V. unguiculata in terms of assimilation and remobilization of stored nitrogen resulted in a seed yield 28% greater than that of P. vulgaris. P. vulgaris showed a negative correlation between the nitrate-reductase activity and the ureide content of the sap indicating that the metabolic pathways leading to ureide production operates alternatively to nitrate assimilation.

  4. Free cyclitol, soluble carbohydrate and protein contents in Vigna unguiculata and Phaseolus vulgaris bean sprouts.

    Science.gov (United States)

    Ribeiro, Elane da Silva; Centeno, Danilo da Cruz; Figueiredo-Ribeiro, Rita de Cássia; Fernandes, Kátia Valevski Sales; Xavier-Filho, José; Oliveira, Antônia Elenir Amancio

    2011-04-27

    Seeds sprouts have been used as a good source of basic nutrients and nutraceutical compounds. The high nutritional value of seeds derives from the deposition of compounds during development. However some of these molecules are used in metabolic processes like germination, which leads to a considerable variation in their concentrations once these events are completed. In this work, we investigate the levels of inositols (myo-inositol, D-pinitol and ononitol), soluble carbohydrates and proteins in cotyledons of Phaseolus vulgaris and Vigna unguiculata sprouts. Sprouting increased myo-inositol and glucose content and reduction of raffinose and ononitol was observed. The protein levels increased in P. vulgaris and decreased in V. unguiculata sprouting. The level of sucrose was maintained in both sprouts. D-Pinitol was detected only in quiescent seeds. Our results suggested that bean sprout is an important source of proteins, sucrose, glucose and myo-inositol. Additionally, bean sprouts have low levels of raffinose, an antinutritional compound.

  5. Occurrence of isoflavonoids in Brazilian common bean germplasm (Phaseolus vulgaris L.).

    Science.gov (United States)

    de Lima, Paula Feliciano; Colombo, Carlos Augusto; Chiorato, Alisson Fernando; Yamaguchi, Lydia Fumiko; Kato, Massuo Jorge; Carbonell, Sérgio Augusto Morais

    2014-10-08

    Common bean (Phaseolus vulgaris) is present in the daily diet of various countries and, as for other legumes, has been investigated for its nutraceutical potential. Thus, 16 genotypes from different gene pools, representing seven types of seed coats and different responses to pathogens and pests, were selected to verify their isoflavone contents. The isoflavonoids daidzein and genistein and the flavonols kaempferol, myricetin, and quercetin were found. Grains of the black type showed the highest concentrations of isoflavonoids and were the only ones to exhibit daidzein. IAC Formoso, with high protein content and source of resistance to anthracnose, showed the greatest concentration of genistein, representing around 11% of the content present in soybean, as well as high levels of kaempferol. Arc 1, Raz 55, and IAC Una genotypes showed high content of coumestrol. The results suggest the use of IAC Formoso to increase the nutraceutical characteristics in common bean.

  6. Pinto Beans (Phaseolus vulgaris L. as a Functional Food: Implications on Human Health

    Directory of Open Access Journals (Sweden)

    Vicki Schlegel

    2013-02-01

    Full Text Available Most foods are considered functional in terms of providing nutrients and energy to sustain daily life, but dietary systems that are capable of preventing or remediating a stressed or diseased state are classified as functional foods. Dry beans (Phaseolus vulgaris L. contain high levels of chemically diverse components (phenols, resistance starch, vitamins, fructooligosaccharides that have shown to protect against such conditions as oxidative stress, cardiovascular disease, diabetes, metabolic syndrome, and many types of cancer, thereby positioning this legume as an excellent functional food. Moreover, the United States has a rich dry bean history and is currently a top producer of dry beans in the world with pinto beans accounting for the vast majority. Despite these attributes, dry bean consumption in the US remains relatively low. Therefore, the objective of this manuscript is to review dry beans as an important US agricultural crop and as functional food for the present age with an emphasis on pinto beans.

  7. Genetic control of orange hilum corona of carioca beans (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Juarez Pires Tomaz

    2007-01-01

    Full Text Available The purpose of this research was to elucidate the genetic control of orange corona color in carioca common beans (Phaseolus vulgaris. We made four crosses between carioca group cultivars that differed in respect to the presence or absence of an orange hilum corona color. The F2, F3, F1BC11, F1BC21, F2BC11 and F2BC21 phenotypic segregations were evaluated with a chi-square test which fitted with the hypothesis that one gene with a dominant allele is responsible for the orange corona color. All generations resulting from the four different crosses showed segregation patterns which agreed with the expected proportions. Our results show that the dominant G allele controls orange corona color in the carioca bean group.

  8. Zolfino landrace (Phaseolus vulgaris L. from Pratomagno: general and specific features of a functional food

    Directory of Open Access Journals (Sweden)

    Francesco Balestri

    2016-07-01

    Full Text Available Background: The Zolfino bean is a variety of Phaseolus vulgaris, which is cultivated in a limited area of Tuscany, Italy, and is widely appreciated for its flavor and culinary uses. Objectives: A yellow Zolfino landrace cultivated in the Leccio-Reggello area was characterized and compared with three other varieties of Phaseolus vulgaris (i.e. the Borlotto, Cannellino, and Corona beans in terms of its general features and potential as an antioxidant/anti-inflammatory agent. Design: The length, width, thickness, equatorial section surface, weight, volume, and seed coat section were measured in all the beans. The seed surface area was also estimated by an original empirical method. The ability of the different beans to interfere with the enzymes of the polyol pathway (that is, aldose reductase (AR and sorbitol dehydrogenase was tested using the supernatant after soaking the beans at room temperature and after thermal treatment, which simulated the bean-cooking process in a controlled fashion. Results: Concerning the general features, Zolfino was comparable with other beans, except Corona, in terms of surface–volume ratio, which possesses the lowest tegument thickness. Moreover, Zolfino appears the most effective in inhibiting AR activity. The inhibitory ability is unaffected by thermal treatment and appears to be associated with compound(s present in the coat of the bean. Conclusions: The ability of Zolfino to inhibit AR, thus reducing the flux of glucose through the polyol pathway, highlights the features of Zolfino as a functional food, potentially useful in treating the dysfunctions linked to the hyperactivity of AR, such as diabetic complications or inflammatory responses.

  9. Evolutionary history of mitogen-activated protein kinase (MAPK) genes in Lotus, Medicago, and Phaseolus.

    Science.gov (United States)

    Neupane, Achal; Nepal, Madhav P; Benson, Benjamin V; Macarthur, Kenton J; Piya, Sarbottam

    2013-11-01

    Mitogen-Activated Protein Kinase (MAPK) genes encode proteins that mediate various signaling pathways associated with biotic and abiotic stress responses in eukaryotes. The MAPK genes form a 3-tier signal transduction cascade between cellular stimuli and physiological responses. Recent identification of soybean MAPKs and availability of genome sequences from other legume species allowed us to identify their MAPK genes. The main objectives of this study were to identify MAPKs in 3 legume species, Lotus japonicus, Medicago truncatula, and Phaseolus vulgaris, and to assess their phylogenetic relationships. We used approaches in comparative genomics for MAPK gene identification and named the newly identified genes following Arabidopsis MAPK nomenclature model. We identified 19, 18, and 15 MAPKs and 7, 4, and 9 MAPKKs in the genome of Lotus japonicus, Medicago truncatula, and Phaseolus vulgaris, respectively. Within clade placement of MAPKs and MAPKKs in the 3 legume species were consistent with those in soybean and Arabidopsis. Among 5 clades of MAPKs, 4 founder clades were consistent to MAPKs of other plant species and orthologs of MAPK genes in the fifth clade-"Clade E" were consistent with those in soybean. Our results also indicated that some gene duplication events might have occurred prior to eudicot-monocot divergence. Highly diversified MAPKs in soybean relative to those in 3 other legume species are attributable to the polyploidization events in soybean. The identification of the MAPK genes in the legume species is important for the legume crop improvement; and evolutionary relationships and functional divergence of these gene members provide insights into plant genome evolution.

  10. Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua.

    Science.gov (United States)

    Marcenaro, Delfia; Valkonen, Jari P T

    2016-01-01

    Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, 'INTA Rojo'. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions. Eighty-seven isolates caused symptoms of varying severity in the seedlings, including discoloration, necrotic lesions, cankers, rot, and lethal necrosis. Pathogenic isolates were divided into eight phenotypically distinguishable groups based on morphology and growth characteristics on artificial growth medium, and further identified by analysis of the internal transcribed spacer sequences (ITS1 and ITS2) of the ribosomal RNA genes. The pathogenic isolates belonged to eight genera. Fusarium spp. (F. chlamydosporum, F. equiseti, F. incarnatum), Lasiodiplodia theobromae, Macrophomina phaseolina, and Penicillium citrinum were the most damaging and common fungi found in the seed lots. Furthermore, Corynespora cassiicola, Colletotrichum capsisi, Colletotrichum gloeosporioides, Aspergillus flavus, and Diaporthe sp. (Phomopsis) were seedborne in cultivar 'INTA Rojo' and found to be pathogenic to bean seedlings. This study reveals, for the first time, many seedborne pathogenic fungi in beans in Nicaragua; furthermore, prior to this study, little information was available concerning F. equiseti, F. incarnatum, L. theobromae, C. cassiicola, and Diaporthe spp. as seedborne pathogens of common bean. Our results lay the basis for developing diagnostic tools for seed health inspection and for further study of the epidemiology

  11. Zolfino landrace (Phaseolus vulgaris L.) from Pratomagno: general and specific features of a functional food

    Science.gov (United States)

    Balestri, Francesco; Rotondo, Rossella; Moschini, Roberta; Pellegrino, Mario; Cappiello, Mario; Barracco, Vito; Misuri, Livia; Sorce, Carlo; Andreucci, Andrea; Del-Corso, Antonella; Mura, Umberto

    2016-01-01

    Background The Zolfino bean is a variety of Phaseolus vulgaris, which is cultivated in a limited area of Tuscany, Italy, and is widely appreciated for its flavor and culinary uses. Objectives A yellow Zolfino landrace cultivated in the Leccio-Reggello area was characterized and compared with three other varieties of Phaseolus vulgaris (i.e. the Borlotto, Cannellino, and Corona beans) in terms of its general features and potential as an antioxidant/anti-inflammatory agent. Design The length, width, thickness, equatorial section surface, weight, volume, and seed coat section were measured in all the beans. The seed surface area was also estimated by an original empirical method. The ability of the different beans to interfere with the enzymes of the polyol pathway (that is, aldose reductase (AR) and sorbitol dehydrogenase) was tested using the supernatant after soaking the beans at room temperature and after thermal treatment, which simulated the bean-cooking process in a controlled fashion. Results Concerning the general features, Zolfino was comparable with other beans, except Corona, in terms of surface–volume ratio, which possesses the lowest tegument thickness. Moreover, Zolfino appears the most effective in inhibiting AR activity. The inhibitory ability is unaffected by thermal treatment and appears to be associated with compound(s) present in the coat of the bean. Conclusions The ability of Zolfino to inhibit AR, thus reducing the flux of glucose through the polyol pathway, highlights the features of Zolfino as a functional food, potentially useful in treating the dysfunctions linked to the hyperactivity of AR, such as diabetic complications or inflammatory responses. PMID:27415159

  12. Localization of phytase transcripts in germinating seeds of the common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Lazali, Mohamed; Louadj, Lamia; Ounane, Ghania; Abadie, Josiane; Amenc, Laurie; Bargaz, Adnane; Lullien-Pellerin, Valérie; Drevon, Jean-Jacques

    2014-09-01

    The work provides the first-time evidence of tissue-specific expression of a phytase gene in the germinating seeds of Phaseolus vulgaris. Phytase enzyme plays a major role in germinating seeds. It is also active during N2 fixation within nodules of legumes. The effect of phosphorus (P) deficiency on phytase gene expression and localization in N2-fixing root nodules has been recently studied in hydroaeroponic culture of Phaseolus vulgaris. In this study, phytase gene transcripts within the germinating seed tissues of the P-inefficient P. vulgaris recombinant inbred line RIL147 were in situ localized with a similar RT-PCR recipe as that used for nodules. Our results show that the phytase gene expression was mainly localized in the outer layers, vascular cells and parenchyma of germinating seeds whereas it was localized in the inner and middle cortex of nodules. Image analysis quantified higher fluorescence intensity of the phytase transcript signal in the seed embryo than in radicles, cotyledons or the nodule cortex. Furthermore, the phytase activity was 22-fold higher in cotyledons (43 nmol min(-1) g(-1) dry weight) than in nodules (2 nmol min(-1) g(-1) dry weight). The K m and V m values of phytase activity in cotyledons were also significantly higher than in nodules. Interestingly, the amplified sequence of cDNA phytase exhibited highest homology with the Glycine max purple acid phosphatase (NM_001289274) 90 % for germinating seed as compared to nodule phytase cDNA displaying 94 % homology with the Glycine max phytase (GQ422774.1). It is concluded that phytase enzymes are likely to vary from seeds to nodules and that phytase enzymes play key roles in the use of organic P or N2 fixation, as it is well known for germination.

  13. Purification, characterization and induction of L-phenylalanine ammonia-lyase in Phaseolus vulgaris.

    Science.gov (United States)

    da Cunha, A

    1988-12-01

    The enzyme L-phenylalanine ammonia-lyase was purified from leaves of Phaseolus vulgaris by Sephacryl S-200 gel filtration and Sepharose-4-B--succinyl-aminoethyl-L-phenylalanine affinity chromatography. L-Phenylalanine ammonia-lyase was specifically eluted from the affinity matrix with its substrate L-phenylalanine at 20-25 degrees C. The purified enzyme was shown to be homogeneous by gel electrophoresis both in presence and absence of SDS. Its Mr, determined by gel filtration and non-denaturing gel electrophoresis, was 320,000 +/- 9000 and 330,000 +/- 4000 respectively. After SDS electrophoresis only one band of Mr 83,000 +/- 4000 was detected, indicating that the enzyme is an oligomer containing four subunits. The pH optimum of enzyme activity was 8.8-9.2. Ampholyte isoelectrofocusing in polyacrylamide demonstrated the presence of a single charged species at pH 4.2. The homogeneous enzyme catalyzed the deamination of L-phenylalanine to trans-cinnamate but did not catalyze the transamination of L-phenylalanine to L-phenylpyruvate. The enzyme showed Km 1.25 mM for L-phenylalanine. Antibodies to homogeneous L-phenylalanine ammonia-lyase recognised specific epitopes on L-phenylalanine aminotransferase as demonstrated by immunoaffinity purification and immunoblotting. The induction of L-phenylalanine ammonia-lyase activity during phaseollin biosynthesis in the Phaseolus vulgaris--Colletotrichum lindemuthianum interaction was regulated by an increase in enzyme concentration resulting from an increase in de novo synthesis of L-phenylalanine ammonia-lyase protein.

  14. FORAGE PRODUCTIVITY OF ARBILA (Phaseolus lunatus AT VARIOUS LEVELS OF RHIZOBIUM INOCULANTS AND HARVESTING TIMES

    Directory of Open Access Journals (Sweden)

    B.B. Koten

    2014-10-01

    Full Text Available The study was conducted to evaluate forage productivity of arbila (Phaseolus lunatus as theruminant feed at various levels of rhizobium inoculants and harvesting times, was designed followingcompletely randomized design of factorial pattern with two factors. The first factor was the level ofinoculums (I i.e. I1 (without inoculum, I2 (5 g/kg seed, I3 (10 g/kg seed, and I4 (15 g/kg seed. Thesecond factor was harvesting time (U i.e. U1 (60 days, U2 (80 days, U3 (100 days with 4replications. The variables were N uptake, production of dry matter (DM and organic matter (OM, andpercentage of OM, crude protein (CP, crude fiber (CF, nitrogen free extract (NFE, extract ether (EEand ash of arbila forage (based on DM. The results showed that inoculant treatment at the rate of 15g/kg seed produced the highest percentage of effective nodules (98.72%, and in combination withharvesting age at 100 days each polybag of plant was able to absorb the higest amount of N (688. 10 gwith production of DM 273.81 g, OM production 263.96 g and nutrients content based on DM of about91.14% OM, 33.52% CF, 34.76%, 5.75% EE, 09.37% ash, and 16.16% CP. From the study, it can beconcluded that arbila plants inoculated specific rhizobium Phaseolus vulgaris at the rate of 15 g/kg seedand harvested at 100 days yielded the best forage as feed.

  15. Physicochemical properties of cowpea (Vigna unguiculata L. Walp. meals and their apparent digestibility in white shrimp (Litopenaeus vannamei Boone Propiedades fisicoquímicas de harinas de frijol yorimón (Vigna unguiculata L. Walp. y su digestibilidad aparente en camarón Litopenaeus vannamei Boone

    Directory of Open Access Journals (Sweden)

    Martha Elisa Rivas-Vega

    2009-04-01

    Full Text Available The effect of different feed processing methods on the physicochemical properties, and apparent digestibility of cowpea (Vigna unguiculata meals as ingredients in diets for white shrimp (Litopenaeus vannamei was investigated. Five experimental cowpea meals were prepared: whole raw (WRC, dehulled (DC, cooked (CC, germinated (GC and extruded (EXC. The physicochemical properties of the meals were evaluated using differential scanning calorimetry. The meals were included at 15% in diets for L. vannamei (15.4 g to determine firmness of pellets and in vivo digestibility of nutrients by using chromic oxide as inert marker. Six diets were evaluated: a control diet, and five diets containing the different cowpea meals. Transition enthalpy significantly decreased after thermal treatment, from 6.1 J/g in WRC to 1.4 J/g in CC, and disappeared in EXC. Firmness of pellets varied from 1.1 N in the EXC diet to 2.8 N in the WRC diet. A significant negative correlation between transition enthalpy and carbohydrate digestibility was found. Dry matter, protein, carbohydrate and lipid digestibility of cowpea meals significantly increased after germinating, cooking or extruding. It is concluded that germinated, cooked and extruded cowpea meals are highly digestible for shrimp and that enthalpy of transition is negatively correlated with the digestibility of carbohydrates.Se evaluó el efecto de diferentes procesos sobre las propiedades fisicoquímicas y digestibilidad aparente de la harina de frijol yorimón (Vigna unguiculata como ingrediente en alimentos para camarón blanco (Litopenaeus vannamei. Se elaboraron cinco harinas de frijol yorimón: entero crudo (WRC, decorticado (DC, cocido (CC, germinado (GC y extruido (EXC. Las características térmicas de las harinas fueron evaluadas usando calorimetría diferencial de barrido. Se elaboraron seis alimentos experimentales: un alimento control y cinco alimentos conteniendo 15% de las diferentes harinas de frijol yorim

  16. Colorado Fathers' Resource Guide = Guia de Recursos para los Padres en Colorado.

    Science.gov (United States)

    Colorado Foundation for Families and Children, Denver.

    Developed through the Colorado Fatherhood Connection, this guide, in English- and Spanish-language versions, provides suggestions and resources for fathers as well as tips on discipline, communication, and activities fathers can do with their children. Topics addressed in the guide include characteristics of responsible fatherhood, characteristics…

  17. Soil moisture ground truth: Steamboat Springs, Colorado, site and Walden, Colorado, site

    Science.gov (United States)

    Jones, E. B.

    1976-01-01

    Ground-truth data taken at Steamboat Springs and Walden, Colorado in support of the NASA missions in these areas during the period March 8, 1976 through March 11, 1976 was presented. This includes the following information: snow course data for Steamboat Springs and Walden, snow pit and snow quality data for Steamboat Springs, and soil moisture report.

  18. 78 FR 19296 - Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO

    Science.gov (United States)

    2013-03-29

    ... appropriate Indian tribes, and has determined that there is no cultural affiliation between the human remains...: Representatives of any Indian tribe that believes it has a cultural affiliation with the human remains should... of History Colorado, Denver, CO. The human remains were removed from Suncor Energy USA Pipeline...

  19. 76 FR 17444 - Notice of Inventory Completion: Colorado Historical Society (History Colorado), Denver, CO

    Science.gov (United States)

    2011-03-29

    ... (History Colorado), Denver, CO. The human remains were removed from Howiri Ruin (LA 71), Taos County, NM...), Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. Notice is here given in accordance... Ana, New Mexico; Pueblo of Santa Clara, New Mexico; Pueblo of Taos, New Mexico; Pueblo of Tesuque,...

  20. 76 FR 28071 - Notice of Inventory Completion: Colorado Historical Society (History Colorado), Denver, CO

    Science.gov (United States)

    2011-05-13

    ...), Denver, CO AGENCY: National Park Service, Interior. ACTION: Notice. Notice is here given in accordance... (History Colorado), Denver, CO. The human remains were removed from Canyon de Chelly, AZ. This notice is... Mexico; Pueblo of Santa Ana, New Mexico; Pueblo of Santa Clara, New Mexico; Pueblo of Taos, New...

  1. 78 FR 72700 - Notice of Inventory Completion: History Colorado, formerly Colorado Historical Society, Denver, CO

    Science.gov (United States)

    2013-12-03

    ... 1973, he brought the human remains to the Department of Anthropology at the University of Southern Colorado. In 2000, when the University closed its anthropology lab, the remains were taken into custody by.... 3001(9), the human remains described in this notice represent the physical remains of one individual of...

  2. Colorado Hispanics: A Report of Selected Social Concerns, 1992.

    Science.gov (United States)

    Pappas, Georgia, Ed.; Guajardo, Maria, Ed.

    This publication offers a compilation of 12 reports on selected social concerns pertaining to the Hispanic community in Colorado and provides a comprehensive overview of demographic information and information on health, education, and social welfare issues. The first report looks at Colorado's multicultural population through a demographic…

  3. Colorado River Basin Hover Dam - Review of Flood Control Regulation.

    Science.gov (United States)

    1982-07-01

    Percichthyidae Striped bass 1ile sxiiis Pocilldae Mosquito fish Cainbusia affnus Sailfin mollie Poecilia latipin a Mexican mollie Poecila mexicana Salmonidae...Colorado River Basin Progress Report No. 8, 195 pp. Vitt, L.J. and R.D. Ohmart, 1978. Herpetofauna of the Lower Colorado River: Davis Dam to the

  4. Colorado Air Quality Control Regulations and Ambient Air Quality Standards.

    Science.gov (United States)

    Colorado State Dept. of Health, Denver. Div. of Air Pollution Control.

    Regulations and standards relative to air quality control in Colorado are defined in this publication. Presented first are definitions of terms, a statement of intent, and general provisions applicable to all emission control regulations adopted by the Colorado Air Pollution Control Commission. Following this, three regulations are enumerated: (1)…

  5. Extensive Green Roof Research Program at Colorado State University

    Science.gov (United States)

    In the high elevation, semi-arid climate of Colorado, green roofs have not been scientifically tested. This research examined alternative plant species, media blends, and plant interactions on an existing modular extensive green roof in Denver, Colorado. Six plant species were ev...

  6. 77 FR 21803 - Notice of Competitive Coal Lease Sale, Colorado

    Science.gov (United States)

    2012-04-11

    ... Bureau of Land Management Notice of Competitive Coal Lease Sale, Colorado AGENCY: Bureau of Land... Creek East Tract described below in Gunnison County, Colorado, will be offered for competitive lease by... lease sale will be held at 10 a.m., May 15, 2012. The sealed bid must be submitted on or before 10...

  7. POLIAMINAS E ATIVIDADE DA PEROXIDASE EM FEIJÃO (Phaseolus vulgaris L. CULTIVADO SOB ESTRESSE SALINO POLYAMINES AND PEROXIDASE ACTIVITY IN BEAN (Phaseolus vulgaris L. GROWN UNDER SALINE STRESS

    Directory of Open Access Journals (Sweden)

    Giuseppina Pace Pereira Lima

    1999-01-01

    Full Text Available O teor de poliaminas (putrescina, espermidina e espermina e a atividade enzimática da peroxidase (EC 1.11.1.7 foram determinados em plantas de Phaseolus vulgaris L. cv Carioquinha, após terem sido submetidas a estresse salino (50 e 100 mM de NaCl. Foram observadas alterações nos teores das poliaminas, principalmente putrescina, que aumentou com o tempo e a concentração de NaCl. Também ocorreu aumento na atividade da peroxidase em ambas concentrações de NaCl utilizadas. Os resultados mostraram alterações no metabolismo de poliaminas e peroxidases nas plantas de feijão cultivadas em meio salino.Putrescine, spermidine and spermine contents and peroxidase activity (EC 1.11.1.7 were determined in plants of Phaseolus vulgaris L. cv Carioquinha submitted to saline stress (50 and 100 mM NaCl. Changes were observed in polyamine contents, mainly in putrescine. Increase in peroxidase activity also occurred with NaCl concentration, for both concentrations used. The results showed alterations in the polyamines contents and peroxidase activity in plants of Phaseolus vulgaris grown under salinity.

  8. Study on Plantago major L. dan Phaseolus vulgaris L. chlorophyll and carotenoid content using as bioincator for air pollution

    Directory of Open Access Journals (Sweden)

    ENDANG ANGGARWULAN

    2007-10-01

    Full Text Available The aims of this research were to study using chlorophyll and carotenoid as bioindicator air quality. This research used completely randomized design 2 x 4 factorial with 5 replicates. The first factor was distance from source of exhaust automobile emissions, consists of 4 levels: 0,50, 100, and 200 m. The second factor was plant spesies, consist 2 level: Plantago major and Phaseolus vulgaris. Data collected were analyzed using Multiple Regression Analysis followed by Duncan Multiple Range Test in 5% confidence level. The result indicated that increasing distance from source exhaust automobile emission, increased growth and chlorophyll content. Chlorophyll content in Phaseolus is more sensitive as bioindicator for air pollution.

  9. Development of industrial minerals in Colorado

    Science.gov (United States)

    Arbogast, Belinda F.; Knepper, Daniel H.; Langer, William H.; Cappa, James A.; Keller, John W.; Widmann, Beth L.; Ellefsen, Karl J.; Klein, Terry L.; Lucius, Jeffrey E.; Dersch, John S.

    2011-01-01

    Technology and engineering have helped make mining safer and cleaner for both humans and the environment. Inevitably, mineral development entails costs as well as benefits. Developing a mine is an environmental, engineering, and planning challenge that must conform to many Federal, State, and local regulations. Community collaboration, creative design, and best management practices of sustainability and biodiversity can be positive indicators for the mining industry. A better understanding of aesthetics, culture, economics, geology, climate, vegetation and wildlife, topography, historical significance, and regional land planning is important in resolving land-use issues and managing mineral resources wisely. Ultimately, the consuming public makes choices about product use (including water, food, highways, housing, and thousands of other items) that influence operations of the mineral industry. Land planners, resource managers, earth scientists, designers, and public groups have a responsibility to consider sound scientific information, society's needs, and community appeals in making smart decisions concerning resource use and how complex landscapes should change. An effort to provide comprehensive geosciences data for land management agencies in central Colorado was undertaken in 2003 by scientists of the U.S. Geological Survey and the Colorado Geological Survey. This effort, the Central Colorado Assessment Project, addressed a variety of land-use issues: an understanding of the availability of industrial and metallic rocks and minerals, the geochemical and environmental effects of historic mining activity on surface water and groundwater, and the geologic controls on the availability and quality of groundwater. The USDA Forest Service and other land management agencies have the opportunity to contribute to the sustainable management of natural aggregate and other mineral resources through the identification and selective development of mineral resources and the

  10. Effect of paper mill effluent on germination of green gram (Phaseolus aureus Roxb.) and growth behaviour of it's seedlings.

    Science.gov (United States)

    Malla, Luna; Mohanty, B K

    2005-06-01

    Effect of paper mill effluents on Phaseolus aureus Roxb was studied. The effluent significantly inhibited germination of root and shoot length. The bio-chemical injury does not appear spontaneously but with the increase in effluent treatment there is reduction in observed biochemical parameters (chlorophyll, protein, amino acid, nuclic acids and carbohydrate) which are negatively correlated. The shoots of the seedlings were found to be resistant; whereas roots of the seedlings were susceptible to paper mill effluent treatment.

  11. Physiological and molecular analysis of the interaction between aluminium toxicity and drought stress in common bean (Phaseolus vulgaris)

    OpenAIRE

    Yang, Zhong-Bao; Eticha, Dejene; Albacete, Alfonso; Madhusudana Rao, Idupulapati; Roitsch, Thomas; Horst, Walter Johannes

    2012-01-01

    Aluminium (Al) toxicity and drought are two major factors limiting common bean (Phaseolus vulgaris) production in the tropics. Short-term effects of Al toxicity and drought stress on root growth in acid, Al-toxic soil were studied, with special emphasis on Al-drought interaction in the root apex. Root elongation was inhibited by both Al and drought. Combined stresses resulted in a more severe inhibition of root elongation than either stress alone. This result was different from the alleviatio...

  12. Response- Surface Analysis for Evaluation of Competition in Different Densities of Sesame (Sesamum indicum) and Bean (Phaseolus vulgaris) Intercropping

    OpenAIRE

    Koocheki, A.; M. Nassiri Mahalati; Y Alizadeh; R. Moradi

    2014-01-01

    Response surface models predict crop yield based on crop density and this is an important tool for evaluation competition at different density and hence selection of optimum density based on yield. In order to study intra and inter specific competition in intercropping bean (Phaseolus vulgaris) and sesame (Sesamum indicum), an experiment was conducted at the Agricultural Research Station, Ferdowsi University of Mashhad during the growing season of 2010. For this purpose a complete randomized ...

  13. Colorado Front Range Surface Ozone Analysis

    Science.gov (United States)

    McClure-Begley, A.; Petropavlovskikh, I. V.; Oltmans, S. J.; Kofler, J.; Petron, G.; Cothrel, H.

    2014-12-01

    The Colorado Front Range is a unique geographical region for air quality studies, including research of surface level ozone. Not only does surface ozone play a critical role in regulating the oxidation capacity of the atmosphere, but is a primary contributor to local smog and leads to public health complications and altered ecosystem functioning. The high frequency of sunny days, increasing population and pollution, and Mountain/Valley air dynamics of this region provide atmospheric conditions suitable for production and accumulation of ozone at the surface. This region of Colorado is currently in an ozone non-attainment status due to an assortment of contributing factors. Precursor emissions from pollution, wild-fires, and gas and oil production; along with stratosphere-troposphere exchange, can all result in high ozone episodes over the Colorado Front Range. To understand the dynamics of ozone accumulation in this region, Thermo-Scientific ozone monitors have been continuously sampling ozone from 4 different altitudes since the early 2000s. Analysis of ozone data in relation to Nitrogen Oxides (NOx), Methane (CH4), Carbon Monoxide (CO), wind-conditions and back-trajectory air mass origins help to address local ozone precursor emissions and resulting high ozone episodes. Increased ozone episodes are scrutinized with regards to dominant wind direction to determine main precursor emission sources. Analysis of this data reveals a strong influence of precursor emissions from the North-East wind sector, with roughly 50% of ozone exceedances originating from winds prevailing from this direction. Further, correlation with methane is enhanced when prevailing winds are from the North-East; indicative of influence from natural gas processes and feedlot activity. Similar analysis is completed for the North-West wind sector exceedances, with strong correlation to carbon monoxide; likely related to emissions from biomass burning events and forest fires. In depth analysis of

  14. Rawhide Energy Station, Fort Collins, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Peltier, R.

    2008-10-15

    The staff of Platte River Power Authority's Rawhide Energy Station have been racking up operating stats and an environmental performance record that is the envy of other plant managers. In the past decade Rawhide has enjoyed an equivalent availability factor in the mid to high 90s and an average capacity factor approaching 90%. Still not content with this performance, Rawhide invested in new technology and equipment upgrades to further optimise performance, reduce emissions, and keep cost competitive. The Energy Station includes four GE France 7EA natural gas-fired turbines totalling 260 MW and a 274 MW coal-fired unit located in northeastern Colorado. 7 figs.

  15. 77 FR 9840 - Amendment of Class E Airspace; Colorado Springs, CO

    Science.gov (United States)

    2012-02-21

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Colorado Springs, CO AGENCY... airspace at City of Colorado Springs Municipal Airport, Colorado Springs, CO. Decommissioning of the Black... controlled airspace at Colorado Springs, CO (76 FR 70920). Interested parties were invited to participate...

  16. Epidemiologic characterization of Colorado backyard bird flocks.

    Science.gov (United States)

    Smith, Emily I; Reif, John S; Hill, Ashley E; Slota, Katharine E; Miller, Ryan S; Bjork, Kathe E; Pabilonia, Kristy L

    2012-06-01

    Backyard gallinaceous bird flocks may play an important role in the spread of infectious diseases within poultry populations as well as the transmission of zoonotic diseases to humans. An epidemiologic characterization was conducted of Colorado backyard flocks to gather information on general flock characteristics, human movement of birds, human-bird interaction, biosecurity practices, and flock health. Our results suggest that backyard poultry flocks in Colorado are small-sized flocks (68.6% of flocks had backyard flock environment may promote bird-to-bird transmission as well as bird-to-human transmission of infectious disease. Birds are primarily housed with free access to the outside (96.85%), and many are moved from the home premises (46.06% within 1 yr). Human contact with backyard flocks is high, biosecurity practices are minimal, and bird health is negatively impacted by increased movement events. Increased knowledge of backyard bird characteristics and associated management practices can provide guidelines for the development of measures to decrease disease transmission between bird populations, decrease disease transmission from birds to humans, and increase the overall health of backyard birds.

  17. Fluorescence In Situ Hybridization (FISH-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris and Relatives

    Directory of Open Access Journals (Sweden)

    Aiko Iwata-Otsubo

    2016-04-01

    Full Text Available Fluorescence in situ hybridization (FISH-based karyotyping is a powerful cytogenetics tool to study chromosome organization, behavior, and chromosome evolution. Here, we developed a FISH-based karyotyping system using a probe mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and chromosome-specific BAC clones in common bean, which enables one to unambiguously distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping system to several wild relatives and landraces of common bean from two distinct gene pools, as well as other related Phaseolus species, to investigate repeat evolution in the genus Phaseolus. Comparison of karyotype maps within common bean indicates that chromosomal distribution of the centromeric and subtelomeric satellite repeats is stable, whereas the copy number of the repeats was variable, indicating rapid amplification/reduction of the repeats in specific genomic regions. In Phaseolus species that diverged approximately 2–4 million yr ago, copy numbers of centromeric repeats were largely reduced or diverged, and chromosomal distributions have changed, suggesting rapid evolution of centromeric repeats. We also detected variation in the distribution pattern of subtelomeric repeats in Phaseolus species. The FISH-based karyotyping system revealed that satellite repeats are actively and rapidly evolving, forming genomic features unique to individual common bean accessions and Phaseolus species.

  18. Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated bean species as sources of lectin-related resistance factors?

    Science.gov (United States)

    Lioi, L; Galasso, I; Lanave, C; Daminati, M G; Bollini, R; Sparvoli, F

    2007-11-01

    The APA (Arcelin/Phytohemagglutinin/alpha-Amylase inhibitor) gene family is composed of various members, present in Phaseolus species and coding for lectin and lectin-related seed proteins having the double role of storage and defense proteins. Here members of the APA family have been identified by immunological, functional, and molecular analyses and representative genes were sequenced in nine wild species of Phaseolus. All taxa possessed at least one member of the true lectin gene. No arcelin type sequences have been isolated from the species examined. Among the wild species studied, only P. costaricensis contained an alpha-amylase inhibitor (alpha-AI). In addition P. augusti, P. maculatus, P. microcarpus, and P. oligospermus showed the presence of the lectin-related alpha-amylase inhibitor-like (AIL) genes and alpha-AI activity. Data from Southern blot analysis indicated the presence of only one lectin gene in P. parvulus and P. filiformis, while an extensive gene duplication of the APA locus was found in the other Phaseolus species. Phylogenetic analysis carried out on the nucleotide sequences showed the existence of two main clusters and clearly indicated that lectin-related genes originated from a paralogous duplication event preceding the development of the ancestor to the Phaseolus genus. The finding of detectable alpha-AI activity in species containing AIL genes suggests that exploiting APA genes variability in the Phaseolus genus may represent a valuable tool to find new members that may have acquired insecticidal activities.

  19. Health hazard evaluation determination report No. 78-128-549, Nixon Power Plant, Colorado Springs, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Gunter, B.J.

    1978-12-01

    Asbestos (CAS 1332-21-4) concentrations during sanding and buffing operations were measured at the Nixon Power facility (SIC-4911) in Colorado Springs, Colorado on September 29, 1978. The evaluation was requested by the vice president of the Watkin Construction Company on behalf of plumbers engaged in sanding asbestos joints and connections. Breathing zone asbestos concentrations of fibers greater than five microns in length ranged from 0.02 to 0.187 fibers per cubic centimeter. The OSHA asbestos standard of 2 fibers per cubic centimeter was not exceeded, however, the author concludes that a potential asbestos hazard does exist. He recommends that respirators be used by workers until exhaust ventilation is provided.

  20. Cloning and characterization of a biotic-stress-inducible glutathione transferase from Phaseolus vulgaris.

    Science.gov (United States)

    Chronopoulou, Evangelia; Madesis, Panagiotis; Tsaftaris, Athanasios; Labrou, Nikolaos E

    2014-01-01

    Glutathione transferases (GSTs, EC 2.5.1.18) are ubiquitous proteins in plants that play important roles in stress tolerance and in the detoxification of toxic chemicals and metabolites. In this study, we systematically examined the catalytic diversification of a GST isoenzyme from Phaseolus vulgaris (PvGST) which is induced under biotic stress treatment (Uromyces appendiculatus infection). The full-length cDNA of this GST isoenzyme (termed PvGSTU3-3) with complete open reading frame, was isolated using RACE-RT and showed that the deduced amino acid sequence shares high homology with the tau class plant GSTs. PvGSTU3-3 catalyzes several different reactions and exhibits wide substrate specificity. Of particular importance is the finding that the enzyme shows high antioxidant catalytic function and acts as hydroperoxidase, thioltransferase, and dehydroascorbate reductase. In addition, its K m for GSH is about five to ten times lower compared to other plant GSTs, suggesting that PvGSTU3-3 is able to perform efficient catalysis under conditions where the concentration of reduced glutathione is low (e.g., oxidative stress). Its ability to conjugate GSH with isothiocyanates may provide an additional role for this enzyme to act as a regulator of the released isothiocyanates from glucosinolates as a response of biotic stress. Molecular modeling showed that PvGSTU3-3 shares the same overall fold and structural organization with other plant cytosolic GSTs, with major differences at their hydrophobic binding sites (H-sites) and some differences at the level of C-terminal domain and the linker between the C- and N-terminal domains. PvGSTU3-3, in general, exhibits restricted ability to bind xenobiotics in a nonsubstrate manner, suggesting that the biological role of PvGSTU3-3, is restricted mainly to the catalytic function. Our findings highlight the functional and catalytic diversity of plant GSTs and demonstrate their pivotal role for addressing biotic stresses in Phaseolus

  1. Genetic mapping of two genes conferring resistance to powdery mildew in common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Pérez-Vega, Elena; Trabanco, Noemí; Campa, Ana; Ferreira, Juan José

    2013-06-01

    Powdery mildew (PM) is a serious disease in many legume species, including the common bean (Phaseolus vulgaris L.). This study investigated the genetic control behind resistance reaction to PM in the bean genotype, Cornell 49242. The results revealed evidence supporting a qualitative mode of inheritance for resistance and the involvement of two independent genes in the resistance reaction. The location of these resistance genes was investigated in a linkage genetic map developed for the XC RIL population. Contingency tests revealed significant associations for 28 loci out of a total of 329 mapped loci. Fifteen were isolated or formed groups with less than two loci. The thirteen remaining loci were located at three regions in linkage groups Pv04, Pv09, and Pv11. The involvement of Pv09 was discarded due to the observed segregation in the subpopulation obtained from the Xana genotype for the loci located in this region. In contrast, the two subpopulations obtained from the Xana genotype for the BM161 locus, linked to the Co-3/9 anthracnose resistance gene (Pv04), and from the Xana genotype for the SCAReoli locus, linked to the Co-2 anthracnose resistance gene (Pv11), exhibited monogenic segregations, suggesting that both regions were involved in the genetic control of resistance. A genetic dissection was carried out to verify the involvement of both regions in the reaction to PM. Two resistant recombinant lines were selected, according to their genotypes, for the block of loci included in the Co-2 and Co-3/9 regions, and they were crossed with the susceptible parent, Xana. Linkage analysis in the respective F2 populations supported the hypothesis that a dominant gene (Pm1) was located in the linkage group Pv11 and another gene (Pm2) was located in the linkage group Pv04. This is the first report showing the localization of resistance genes against powdery mildew in Phaseolus vulgaris and the results offer the opportunity to increase the efficiency of breeding

  2. Crecimiento y contenido de prolina y carbohidratos de plántulas de frijol sometidas a estrés por sequía Growth and, proline and carbohydrate content of bean seedlings subjected to drought stress

    Directory of Open Access Journals (Sweden)

    Teresa Susana Herrera Flores

    2012-07-01

    Full Text Available El estrés por sequía en el altiplano de México, es un factor limitante en el rendimiento del frijol. En el presente trabajo se estudiaron las respuestas fisiológicas (crecimiento y bioquímicas (carbohidratos solubles y prolina en la radícula de plántulas de frijol resistente, Pinto Villa, y susceptible a la sequía Bayo Madero, ambas de la raza Durango, para identificar características rápidas de medir que sean confiables para selección por resistencia a estrés por sequía. La radícula de las plántulas se sometió a cuatro potenciales de humedad: -2.07, -0.52, -0.27 megapascales (MPa y un testigo -0.16 MPa. Para las determinaciones de carbohidratos solubles y prolina se eliminó el potencial de -0.16 MPa, ya que provocó la pudrición del hipocótilo en el punto de unión con el cotiledón, en este caso el potencial de -0.27 se consideró como testigo. La radícula de ambas variedades tuvo mayor longitud en los tratamientos de menor estrés. En el potencial más crítico, -2.07 Mpa, Pinto Villa, acumuló mayor cantidad de prolina y sacarosa que Bayo Madero, mientras que los niveles de almidón fueron similares. Se observó correlación altamente significativa entre la elongación de la radícula con la acumulación de prolina, glucosa y fructosa (r= 0.99 respectivamente. La acumulación de solutos compatibles (glucosa, fructosa, sacarosa y prolina en la radícula desarrollada en condiciones de estrés, favorece su crecimiento en etapas tempranas de desarrollo. Los solutos compatibles son apropiados como indicadores bioquímicos de selección de variedades resistentes al estrés de humedad durante los primeros tres días de la germinación.Drought stress in the highlands of México is a limiting factor in bean yields. In this work we studied the physiological responses (growth and biochemical (soluble carbohydrates and proline in the radicle of seedlings resistant beans, Pinto Villa and, susceptible to drought Bayo Madero, both of

  3. Influencia de las malezas sobre el cultivo de frijol en función de espaciamiento y de la densidad de plantas Weeds influence in common bean as a function of spacing and plant density

    Directory of Open Access Journals (Sweden)

    M.C Parreira

    2011-12-01

    Full Text Available La hipótesis de investigación es que con la reducción de la densidad de siembra y del espaciamiento entre líneas, el período anterior a la interferencia de las malezas será reducido en el cultivo de frijol. El objetivo de este trabajo fue determinar el período anterior a la interferencia de las malezas (PAI en función del espaciamiento entre líneas y de su densidad poblacional. Los tratamientos fueron constituidos de ocho períodos de convivencia del cultivo con las malezas: 0-10, 0-20, 0-30, 0-40, 0-50, 0-60, 0-70 y 0-80 días después de la emergencia y un control libre de interferencia. Los períodos de convivencia fueron aplicados en dos experimentos, utilizando dos distancias entre líneas, de 0,45 y 0,60 m y en dos densidades de plantas por línea de siembra, de 10 y 15 plantas por metro. El delineamiento experimental utilizado fue en bloques completos al azar, con cuatro repeticiones. Hubo reducción de 16, 40, 36 y 58% en la productividad de granos del cultivo de frijol cuando convivió durante todo el ciclo del cultivo con las malezas, para el espaciamiento de 0,45 m en las densidades de 10 y 15 plantas m-1; y espaciamiento de 0,60 m y densidades de 10 y 15 plantas m-1, respectivamente. La productividad de granos pasó a ser afectada negativamente a partir de 28, 26, 22 y 14 días después de la emergencia, constituyéndose en los períodos anteriores a la interferencia del cultivo, respectivamente.Hypothesis is that reducing the density and the spacing between the lines, the period prior to the interference of weeds will be reduced by the bean crop. The aim of this study was to determine the period prior to weed interference (PPI with the bean crop in terms of row spacing and population density. Treatments consisted of eight periods of weed coexistence with the crop: 0-10, 0-20, 0-30, 0-40, 0-50, 0-60, 0-70 and 0-80 days after crop emergence. The periods of coexistence were applied in two experiments, using two spacings, 0

  4. Flor de Mayo Eugenia, nueva variedad de frijol para riego y temporal en el centro de México Flor de Mayo Eugenia, new bean cultivar for irrigated and rainfall conditions in Central Mexico

    Directory of Open Access Journals (Sweden)

    Jorge Alberto Acosta Gallegos

    2010-12-01

    Full Text Available Dentro del frijol tipo Flor de Mayo, el subtipo 'media oreja' esta cobrando importancia en el norte-centro de México, éste es de tamaño mediano, forma alargada y color rosa atractivo. Se describe una nueva variedad de este tipo de frijol, Flor de Mayo Eugenia (FME. FME se derivó de una cruza interracial entre Flor de Mayo Anita (FMA (raza Jalisco y Rayado Rojo (raza Nueva Granada. El rendimiento promedio de FME en temporal varia de 0.8 a 2.0 t ha-1 y bajo riego el máximo rendimiento ha sido de 3.8 t ha-1. El peso de 100 semillas promedio de diferentes localidades de FME es significativamente superior al de FMA en condiciones de temporal, 32 vs 26 g 100 semillas, y riego 35 vs 31 g 100 semillas. En cuanto la calidad del grano, el tiempo promedio de cocción de FME en cocedor tipo Mattson es 97 min, mientras que el de FMA es de 109 min; el contenido promedio de proteína del grano de FME es 19% en base a peso seco, similar al de FMA, mientras que el contenido de hierro de FME resultó superior al de FMA con 6.1 vs 5 mg 100 g. FME es tolerante a las razas de roya presentes en el Altiplano de México, a la raza 292 de antracnosis y tolerante a los tizones común y de halo.Within 'Flor de Mayo' bean type, the 'media oreja' subtype is becoming important in North-Central México; this last type has a mid-size elongated seed with showy pink color. A new bean cv. of 'media oreja' subtype is described: Flor de Mayo Eugenia (FME. FME was derived from an interracial cross between Flor de Mayo Anita (FMA (Jalisco breed and Rayado Rojo (Nueva Granada breed. The seed yield of FME under rainfall conditions varied from 0.8 to 2.0 t ha-1 and under irrigation the highest yield recorded was 3.8 t ha-1. The 100-seed weight of FME across different locations was significantly superior to the weight of FMA, 32 vs 26 g per 100 seeds under rainfall conditions and 35 vs 31 g per 100 seeds under irrigation conditions. In regard to seed quality, cooking time average of

  5. ANÁLISIS DE LA DIVERSIDAD FENOTÍPICA DE VARIEDADES DE FRIJOL (P. vulgaris L. UTILIZADAS POR LOS CAMPESINOS EN LA COMUNIDAD "LA PALMA" EN PINAR DEL RÍO

    Directory of Open Access Journals (Sweden)

    María E. Viñals

    2002-01-01

    Full Text Available Con el objetivo de evaluar la diversidad fenotípica de las variedades de frijol utilizadas por los campesinos en la comunidad de "La Palma" en Pinar del Río, para su posterior uso en el fitomejoramiento participativo, se desarrolló este experimento en las áreas del Instituto Nacional de Ciencias Agrícolas (INCA, San José de las Lajas. Para su ejecución se seleccionaron 57 materiales de diversos colores y tamaños de granos, que incluyó materiales procedentes de una comunidad campesina en "La Palma", Pinar del Río, del Instituto Nacional de Investigaciones Fundamentales de Agricultura Tropical (INIFAT y materiales donados por el Instituto de Investigaciones Hortícolas "Liliana Dimitrova", los cuales fueron sembrados en épocas temprana y tardía en el período comprendido desde octubre de 1999 hasta abril del 2000 en bloque completamente aleatorizado con cuatro surcos por parcelas de 4 m y con la utilización de mínimos insumos. Se evaluaron las variables: días a la floración, días a la cosecha, rendimiento, peso de 100 semillas, número de semillas/planta y susceptibilidad frente a bacteriosis o roya, según fecha de siembra. Las variables estudiadas se procesaron estadísticamente mediante análisis univariados y multivariados, y de acuerdo con los resultados obtenidos se concluye que los campesinos pueden jugar un papel importante en el mejoramiento de las plantas, ya que las variedades que ellos han seleccionado y utilizan pueden superar a las variedades comerciales y precomerciales.

  6. Aluyori, nueva variedad de frijol blanco (alubia para Sinaloa y el Bajío, México Aluyori, a new dry bean white seeded cultivar (alubia for Sinaloa and El Bajío, Mexico

    Directory of Open Access Journals (Sweden)

    Rafael Atanasio Salinas Pérez

    2010-12-01

    Full Text Available Se describe una nueva variedad de frijol, 'Aluyori'. Esta es la primera variedad de tipo alubia grande desarrollada en México. Aluyori es una variedad de ciclo intermedio de hábito de crecimiento determinado tipo I, de grano grande adecuado para el mercado internacional, con más de 50 g en 100 semillas. Aluyori se adapta a condiciones de riego en los estados de Sinaloa y Sonora donde ha mostrado un potencial de rendimiento de 3 t ha-1 y resistencia a enfermedades. Bajo condiciones de riego Aluyori ha resultado superior en rendimiento a las variedades de tipo Azufrado que comandan el mercado en Sinaloa, a Azufrado Higuera en 18% y a Azufrado Regional 87 en 22%, equivalente a 363 y 430 kg ha-1, respectivamente. Las características culinarias de la variedad Aluyori son similares a las de la principal variedad de grano amarillo, Azufrado Higuera.A new white seeded dry bean cultivar is described, 'Aluyori'. This is the first white kidney type cultivar developed and released in Mexico. Aluyori is a type I growth habit intermediate cycle cultivar, large seeded, suited for the national and international market, its seed weight is above 50 g per 100 seeds. Aluyori is adapted to irrigation conditions in States of Sinaloa and Sonora, as well as El Bajío in Central Mexico where has shown high yield potential of 3 t ha-1 and disease resistance, mostly rust elicited by the fungus. Under irrigation at Sinaloa, Aluyori proved to be superior than leading cultivars in the yellow market class, Azufrado Higuera by 18% and Azufrado Regional 87 by 22%, equivalent to 363 kg ha-1 in relationship to the former and 430 kg ha-1 to the second. The culinary characteristics of Aluyori are similar to those in the leading yellow class cv. Azufrado Higuera.

  7. Conifer health classification for Colorado, 2008

    Science.gov (United States)

    Cole, Christopher J.; Noble, Suzanne M.; Blauer, Steven L.; Friesen, Beverly A.; Curry, Stacy E.; Bauer, Mark A.

    2010-01-01

    Colorado has undergone substantial changes in forests due to urbanization, wildfires, insect-caused tree mortality, and other human and environmental factors. The U.S. Geological Survey Rocky Mountain Geographic Science Center evaluated and developed a methodology for applying remotely-sensed imagery for assessing conifer health in Colorado. Two classes were identified for the purposes of this study: healthy and unhealthy (for example, an area the size of a 30- x 30-m pixel with 20 percent or greater visibly dead trees was defined as ?unhealthy?). Medium-resolution Landsat 5 Thematic Mapper imagery were collected. The normalized, reflectance-converted, cloud-filled Landsat scenes were merged to form a statewide image mosaic, and a Normalized Difference Vegetation Index (NDVI) and Renormalized Difference Infrared Index (RDII) were derived. A supervised maximum likelihood classification was done using the Landsat multispectral bands, the NDVI, the RDII, and 30-m U.S. Geological Survey National Elevation Dataset (NED). The classification was constrained to pixels identified in the updated landcover dataset as coniferous or mixed coniferous/deciduous vegetation. The statewide results were merged with a separate health assessment of Grand County, Colo., produced in late 2008. Sampling and validation was done by collecting field data and high-resolution imagery. The 86 percent overall classification accuracy attained in this study suggests that the data and methods used successfully characterized conifer conditions within Colorado. Although forest conditions for Lodgepole Pine (Pinus contorta) are easily characterized, classification uncertainty exists between healthy/unhealthy Ponderosa Pine (Pinus ponderosa), Pi?on (Pinus edulis), and Juniper (Juniperus sp.) vegetation. Some underestimation of conifer mortality in Summit County is likely, where recent (2008) cloud-free imagery was unavailable. These classification uncertainties are primarily due to the spatial and

  8. Environmental Setting and Implications on Water Quality, Upper Colorado River Basin, Colorado and Utah

    Science.gov (United States)

    Apodaca, Lori E.; Driver, Nancy E.; Stephens, Verlin C.; Spahr, Norman E.

    1995-01-01

    The Upper Colorado River Basin in Colorado and Utah is 1 of 60 study units selected for water-quality assessment as part of the U.S. Geological Survey's National Water-Quality Assessment program, which began full implementation in 1991. Understanding the environmental setting of the Upper Colorado River Basin study unit is important in evaluating water-quality issues in the basin. Natural and human factors that affect water quality in the basin are presented, including an overview of the physiography, climatic conditions, general geology and soils, ecoregions, population, land use, water management and use, hydrologic characteristics, and to the extent possible aquatic biology. These factors have substantial implications on water-quality conditions in the basin. For example, high concentrations of dissolved solids and selenium are present in the natural background water conditions of surface and ground water in parts ofthe basin. In addition, mining, urban, and agricultural land and water uses result in the presence of certain constituents in the surface and ground water of the basin that can detrimentally affect water quality. The environmental setting of the study unit provides a framework of the basin characteristics, which is important in the design of integrated studies of surface water, ground water, and biology.

  9. Quality of life on the Colorado Plateau: a report to the respondents in southwestern Colorado and northwestern New Mexico

    Science.gov (United States)

    Ponds, Phadrea

    2001-01-01

    During the fall of 1998, scientists from the Midcontinent Ecological Science Center (MESC) of the U.S. Geological Survey (USGS) a?? sent a survey by mail to residents in southwest Colorado and northwest New Mexico to better understand quality of life issues in this area of the Colorado Plateau. Collaborators in this study included the Bureau of Land Management and U.S. Forest Service offices located in Durango, Colorado. The information was collected to determine: *what elements of the community and surrounding landscapes contribute to the quality of like among resident populations, and *what critical areas, elements, and special places are essential to retain quality of life.

  10. High elaeophorosis prevalence among harvested Colorado moose.

    Science.gov (United States)

    LeVan, Ivy K; Fox, Karen A; Miller, Michael W

    2013-07-01

    Infection with Elaeophora schneideri, a filarial parasite, occurs commonly in mule deer (Odocoileus hemionus) and elk (Cervus elaphus nelsoni), but seemingly less so in moose (Alces alces). Of 109 carotid artery samples from moose harvested throughout Colorado, USA, in 2007, 14 (13%; 95% binomial confidence interval [bCI]=7-21%) showed gross and 91 (83%; 95% bCI=75-90%) showed histologic evidence of elaeophorosis. Although neither blindness nor other clinical signs associated with elaeophorosis were reported among the harvested moose we examined, the pervasiveness of this parasite may motivate further study of the potential effects of elaeophorosis on moose survival and population performance in the southern Rocky Mountains. Our data suggest histopathology may be more sensitive than gross examination in detecting elaeophorosis in harvested moose.

  11. SANGRE DE CRISTO WILDERNESS STUDY AREA, COLORADO.

    Science.gov (United States)

    Johnson, Bruce R.; Ellis, Clarence E.

    1984-01-01

    Mineral surveys were undertaken of a wilderness study area which includes most of the Sangre de Cristo Range of south-central Colorado. Four areas of probable mineral-resource potential for gold, silver, and base metals lie along a northwest structural trend which follows the western margin of the range north of the Great Sand Dunes National Monument and crosses the range south of the monument. An area of probable mineral-resource potential for similar minerals plus tungsten has been identified east of Blanca Peak at the extreme southern end of the study area. Another area of probable mineral-resource potential includes molybdenum mineralization associated with the Rito Alto stock. A small area of probable geothermal resource potential exists on the west side of the area around the Valley View Hot Springs. There is little promise for the occurrence of oil and gas resources.

  12. US hydropower resource assessment for Colorado

    Energy Technology Data Exchange (ETDEWEB)

    Francfort, J.E.

    1994-05-01

    The US Department of Energy is developing an estimate of the hydropower development potential in this country. Hydropower Evaluation Software (HES) is a computer model that was developed by the Idaho National Engineering Laboratory for this purpose. HES measures the potential hydropower resources available in the United States, using uniform criteria for measurement. The software was developed and tested using hydropower information and data provided by the Southwestern Power Administration. It is a dBASE, menu-driven software application. HES allows the personal computer user to assign environmental attributes to potential hydropower sites, calculate development suitability factors for each site based on the environmental attributes present, and generate reports based on these suitability factors. This report details the resource assessment results for the State of Colorado.

  13. Ecosystem trends in the Colorado Rockies

    Science.gov (United States)

    Stohlgren, T. J.; Baron, J. S.; Kittel, T. G. F.; Binkley, D.

    1995-01-01

    Biological conservation is increasingly moving toward an ecosystem and landscape approach, recognizing the prohibitive cost and difficulty of a species-by-species approach (LaRoe 1993). Also, statewide (e.g., Gap Analysis Program) and national surveys (e.g., Environmental Monitoring and Assessment Program or EMAP) are conducted at a scale and level of resolution that do not meet the needs of most small land-management units that require detailed information at the ecosystem and landscape scale (Stohlgren 1994). The Colorado Rockies are an ideal outdoor laboratory for ecosystem science and management. The escalating environmental threats described in this article compelled us to design a landscape-scale assessment of the status and trends of biotic resources.

  14. Ponnequin Wind Energy Project Weld County, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-08-01

    The purpose of this environmental assessment (EA) is to provide the U.S. Department of Energy (DOE) and the public with information on potential environmental impacts associated with the development of the Ponnequin Wind Energy Project in Colorado. This EA and public comments received on it will be used in DOE`s deliberations on whether to release funding for the project. This document provides a detailed description of the proposed project and an assessment of potential impacts associated with its construction and operations. Resources and conditions considered in the analysis include streams; wetlands; floodplains; water quality; soils; vegetation; air quality; socioeconomic conditions; energy resources; noise; transportation; cultural resources; visual and land use resources; public health and safety; wildlife; threatened, endangered, and candidate species; and cumulative impacts. The analysis found that the project would have minimal impacts on these resources and conditions, and would not create impacts that exceed the significance criteria defined in this document. 90 refs., 5 figs.

  15. Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.)

    Institute of Scientific and Technical Information of China (English)

    Kalpana Nanjareddy; Lourdes Blanco; Manoj-Kumar Arthikala; Xochitl Alvarado Affantrange; Federico Snchez; Miguel Lara

    2014-01-01

    Nitrogen-limited conditions are considered to be a prerequisite for legume-rhizobial symbiosis, but the effects of nitrate-rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial (Rhizobim tropici) and arbusclar mycorrhizal (Glomus intraradices) symbiosis in Phaseolus vulgaris L. cv. Negro Jamapa under nitrate pre-incubation and continuous nitrate conditions. Our results indicate that nitrate pre-incubation, independent of the concentration, did not affect nodule development. However, the continuous supply of nitrate at high concentrations impaired nodule maturation and nodule numbers. Low nitrate conditions, in addition to positively regulating nodule number, biomass, and nitrogenase activity, also extended the span of nitrogen-fixing activity. By contrast, for arbuscular mycorrhizae, continuous 10 and 50 mmol/L nitrate increased the percent root length colonization, concomitantly reduced arbuscule size, and en-hanced ammonia transport without affecting phosphate transport. Therefore, in this manuscript, we have proposed the importance of nitrate as a positive regulator in promoting both rhizobial and mycorrhizal symbiosis in the common bean.

  16. Chemical composition and in vitro polysaccharide fermentation of different beans (Phaseolus vulgaris L.).

    Science.gov (United States)

    Campos-Vega, R; Reynoso-Camacho, R; Pedraza-Aboytes, G; Acosta-Gallegos, J A; Guzman-Maldonado, S H; Paredes-Lopez, O; Oomah, B D; Loarca-Piña, G

    2009-09-01

    The composition of bioactives including polysaccharide yield and resistant starch (RS) content of 4 raw and cooked bean (Phaseolus vulgaris L.) cultivars was evaluated. Polysaccharide was fermented in vitro by incubation with human gut flora under anaerobic conditions and short-chain fatty acids (SCFAs) production was compared at 6, 12, and 24 h by gas chromatography. Polysaccharide and soluble fiber contents increased upon cooking with stachyose as the major oligosaccharide. Cooked bean of cultivar Bayo Madero had the highest yield of polysaccharides (55%) and resistant starch (37%), followed by those of Negro 8025 (48% and 32%, respectively). Acetate was the most abundant SCFAs formed in all bean varieties. The concentration of SCFAs was cultivar-dependent; Bayo Madero and Negro 8025 displayed the highest concentration of butyrate (15 mmol/L), while Azufrado Higuera had the lowest and highest concentrations of acetate (39 mmol/L) and propionate (14 mmol/L), respectively. The results suggest that the common bean is an excellent source of polysaccharides that can be fermented in the colon and produce SCFAs, compounds previously reported to exert health benefits.

  17. Seed germination and seedling growth of bean (Phaseolus vulgaris as influenced by magnetized saline water

    Directory of Open Access Journals (Sweden)

    Fateme Aghamir

    2016-01-01

    Full Text Available Magnetized water is considered eco-friendly physical presowing seed germination.The aim of this study was to evaluate the effects of magnetized watertreatments on bean (Phaseolus vulgaris germination under saline conditions (0, 25, 50, 75, 100 and 120 mM NaCl. This experiment was performed as factorial in a complete randomized design (CRD with three replications. The results revealed that the roots and shoots length, fresh and dry weight of shoots and roots and roots to shoots ratio, chlorophyll content index, water uptake, tissue water contentwere significantly affected by magnetized water.Irrigation with magnetized water significantly increased the physiologic factors such as germination percentage and index, vigor index and salt tolerance index, compared to untreated control seeds.Mean germination time and parameters T1, T10, T25, T50and T90 (required time for germination of one to 90 percent of seeds were reduced significantly in all magnetized water treated plants in comparison to control.The results also demonstrated that magnetized water was conducive to promote the growth of bean seedlings under saline conditions.

  18. Weed Interference Effects on Leaves, Internode and Harvest Index of Dry Bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Hossein GHAMARI

    2015-03-01

    Full Text Available The development of appropriate weed management strategies and efficient use of herbicides relies upon understanding weed-crop interactions. A field study was carried out to assess the effect of weed interference on leaves, internode and harvest index of dry bean (Phaseolus vulgaris L.. The experiment was established under a randomized complete block design with two types of weed interference treatments: plots with weeds and plots without weeds at different time intervals (0, 10, 20, 30, 40 and 50 days after crop emergence. The sigmoid Boltzmann model was used to quantify the crop traits as influenced by weed interference. Prolonged delays in weed removal reduced gradually the number of leaves of the crop. Weed interference decreased dry weight of leaves as well, so that the lowest value of it (33.49 g plant-1 was observed in full season during weed-infested treatment. Infestation of weeds affected the length of the crop internodes. While the weed interference duration increased, the length of the internodes decreased. Harvest index was also sensitive to weed competition. As the crop was kept weed-infested from the emergence for increasing periods of time, harvest index decreased to a value of 28.01%. A significant negative correlation between total biomass of weeds and dry bean traits (number of leaves, leaves dry weight, internode length and harvest index was observed. Therefore, weeds are able to adversely affect dry bean growth through constraining environmental resources and impairing leaves as the photosynthetic areas.

  19. Effects of hydrogen fluoride on plant-pathogen interactions. [Lycopersicon esculentum; Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    McCune, D.C.; Weinstein, L.H.; Mancini, J.F.; van Lueken, P.

    1973-01-01

    Experiments in fumigation chambers with tomato (Lycopersicon esculentum) and pinto bean (Phaseolus vulgaris) plants were performed to assess the effects of hydrogen fluoride on plant-pathogen (fungal and bacterial) interactions. Hydrogen fluoride was found to alter the plant-pathogen interaction, although the kind and consistency of effect caused by HF depended upon the host, pathogen, and several other factors. A reduction in powdery mildew probably indicates that HF was affecting the infectivity of the pathogen itself because reduction in disease was proportional to the length of the exposure period, infection was continuous during the exposure period, and the pathogen itself was epiphytic. The effect of fluoride on bean rust may have been due to accumulated fluoride in the leaf having a direct or indirect effect on the pathogen because both pre- and post-inoculation exposures to HF were effective and additive. Other evidence for an indirect effect of fluoride was found in halo-blight where stem collapse was affected but foliar symptoms were not, and the site affected was spatially removed from the site of fluoride accumulation. Effects on early blight of tomato also indicated an effect of fluoride in the leaf.

  20. Genetic control of common bean (Phaseolus vulgaris resistance to powdery mildew (Erysiphe polygoni

    Directory of Open Access Journals (Sweden)

    Rezende Viviane Ferreira

    1999-01-01

    Full Text Available Genetic control of common bean (Phaseolus vulgaris resistance to powdery mildew (Erysiphe polygoni was studied using segregating populations from the bean variety crosses Jalo x ESAL 686 and ESAL 550 x ESAL 686. F2 plants, together with the parents, were inoculated and evaluated using a scale of values from one (plant without symptoms to nine (completely infected plant. F2 plants were harvested individually, and F2:3 families were obtained. These families were evaluated in an 11 x 11 and 12 x 12 simple lattice statistical design for the Jalo x ESAL 686 and ESAL 550 x ESAL 686 crosses, respectively, using the same value scale as the F2 generation. The segregation observed in F2 plants and F2:3 families indicated that two genes are involved in genetic control, due to a double recessive epistasis. The high linear regression coefficient (b between F2 plants and their F2:3 family, 0.66 for ESAL 550 x ESAL 686 cross, and 0.71 for Jalo x ESAL 686 cross, showed that the trait is highly heritable.

  1. Growth Response of Two Phaseolus mungo L. Cultivars Induced by Arbuscular Mycorrhizal Fungi and Trichoderma viride

    Directory of Open Access Journals (Sweden)

    Navnita Sharma

    2016-01-01

    Full Text Available The present investigation aimed to quantify the difference in response of two Phaseolus mungo L. cultivars (i.e., UH-1 and IPU-94-1 to Glomus mosseae (G, that is, Funneliformis mosseae, Acaulospora laevis (A, and Trichoderma viride (T, in different combinations or alone. All the treatments were inoculated with Bradyrhizobium japonicum to ensure nodulation as soil used in the experiment was sterilized. After 120 days of inoculation, plants were analyzed for chlorophyll content, nodulation, mycorrhization, leaf area, and protein content. Results indicate variation in growth response of two cultivars with different treatments. Triple inoculation of plants with G + A + T proved to be the best treatment for growth followed by G + T in both cultivars. Our work allowed the selection of P. mungo L. cultivar UH-1 as highly mycorrhizal responsive as compared to IPU-94-1 and G. mosseae to be an efficient bioinoculant as compared to A. laevis for growth enhancement of P. mungo. Further characterization of P. mungo genotypes will enhance our knowledge of physiological and genetic mechanism behind increase in plant growth and yield due to AM symbiosis.

  2. Anatomical alterations of Phaseolus vulgaris L. mature leaves irradiated with X-rays.

    Science.gov (United States)

    De Micco, V; Arena, C; Aronne, G

    2014-01-01

    The cultivation of higher plants in Space involves not only the development of new agro-technologies for the design of ecologically closed Space greenhouses, but also understanding of the effects of Space factors on biological systems. Among Space factors, ionising radiation is one of the main constraints to the growth of organisms. In this paper, we analyse the effect of low-LET radiation on leaf histology and cytology in Phaseolus vulgaris L. plants subjected to increasing doses of X-rays (0.3, 10, 50, 100 Gy). Leaves irradiated at tissue maturity were compared with not-irradiated controls. Semi-thin sections of leaves were analysed through light and epi-fluorescence microscopy. Digital image analysis was applied to quantify anatomical parameters, with a specific focus on the occurrence of signs of structural damage as well as alterations at subcellular level, such as the accumulation of phenolic compounds and chloroplast size. Results showed that even at high levels of radiation, general anatomical structure was not severely perturbed. Slight changes in mesophyll density and cell enlargement were detected at the highest level of radiation. However, at 100 Gy, higher levels of phenolic compounds accumulated along chloroplast membranes: this accompanied an increase in number of chloroplasts. The reduced content of chlorophylls at high levels of radiation was associated with reduced size of the chloroplasts. All data are discussed in terms of the possible role of cellular modifications in the maintenance of high radioresistance and photosynthetic efficiency.

  3. Arbuscular mycorrhiza maintains nodule function during external NH4+ supply in Phaseolus vulgaris (L.).

    Science.gov (United States)

    Mortimer, Peter E; Pérez-Fernández, Maria A; Valentine, Alex J

    2012-04-01

    The synergistic benefits of the dual inoculation of legumes with nodule bacteria and arbuscular mycorrhizae (AM) are well established, but the effect of an external NH(4)(+) supply on this tripartite relationship is less clear. This effect of NH(4)(+) supply was investigated with regards to the growth and function of the legume host and both symbionts. Nodulated Phaseolus vulgaris seedlings with and without AM, were grown in a sand medium with either 0 N, 1 mM or 3 mM NH(4)(+). Plants were harvested at 30 days after emergence and measurements were taken for biomass, N(2) fixation, photosynthesis, asparagine concentration, construction costs and N nutrition. The addition of NH(4)(+) led to a decline in the percentage AM colonization and nodule dry weights, although AM colonization was affected to a lesser extent. NH(4)(+) supply also resulted in a decrease in the reliance on biological nitrogen fixation (BNF); however, the AM roots maintained higher levels of NH(4)(+) uptake than their non-AM counterparts. Furthermore, the non-AM plants had a higher production of asparagine than the AM plants. The inhibitory effects of NH(4)(+) on nodule function can be reduced by the presence of AM at moderate levels of NH(4)(+) (1 mM), via improving nodule growth or relieving the asparagine-induced inhibition of BNF. © Springer-Verlag 2011

  4. A growth analysis of waterlogging damage in mung bean (Phaseolus aureus)

    Science.gov (United States)

    Musgrave, M. E.; Vanhoy, M. A.

    1989-01-01

    Mung beans (Phaseolus aureus Roxb.) were grown for 2 weeks in gravel-vermiculite soilless mix in a growth chamber and subjected to a 1-week waterlogging period followed by a 1-week recovery period. Sequential harvests were made to determine the time course of effects of waterlogging and subsequent recovery on growth parameters by techniques of growth analysis. Root dry matter was the first to be affected, along with an increase in leaf dry matter and specific leaf weight. After a 1-week waterlogging period, specific leaf weight had more than doubled in the stressed plants. Leaf area declined in relation to the control plants as did the ratio of root dry matter to shoot dry matter. During the recovery period there was an increase in the dry matter allocation to the roots relative to the shoot. Specific leaf weight fell to control levels although the rate of leaf area elaboration did not increase during this time, suggesting a redistribution of stored assimilates from the leaves. Net assimilation rate increased during the waterlogging period, probably due to a restriction in root metabolism and reduced translocation out of the leaf rather than to an increase in photosynthesis. Net assimilation rate of waterlogged plants was severely reduced compared with control plants during the recovery period. Both relative growth rate and leaf area duration declined during the waterlogging period and declined further subsequent to the waterlogging treatment. The results illustrate the interrelationships between root and shoot carbon budgets in mung bean during response to the stress of waterlogging.

  5. Cytological investigations of mesophyll of Phaseolus vulgaris L. 'Red Kidney' infected with PVS

    Directory of Open Access Journals (Sweden)

    Krystyna Brzezicka-Szymczyk

    2013-12-01

    Full Text Available We have put under cytological observation 2-, 4-, and 8-day fragments of mesophyll of the leaves Phaseolus 'Red Kidney' infected with PVS. In the necrosis caused by PVS can be distinguished a necrotic centre, a seminecrotic zone, and a belt of terminal cells. The necrotic centre is the most changed area whose cells are most often decayed. In the seminecrotic zone, the presence of viruses in the cell causes some pathological changes. The ER canals assume the shape of swollen cisterns. The chloroplasts reacts with the softening of granas, the growth of starch granules and plastoglobules content as well as with the presence of proteinaceous fibrous structures. The karyolymph and mitochondrium matrix become elucidated. The continuity of plasmalemma and tonoplast become interrupted. Virus particles appear in the area of cytoplasm of seminecrotic zone and in the necrotic centre, they are dispersed or rarely arranged in aggregations which often are in contact with tonoplast. We have observed virus packs enclosed with lipoproteid membrane. Viruses were not stated in the terminal zone. We have ascertained the intensive development of cell walls in the cells of terminal and seminecrotic zones. A lobar or punctual way of the development appeared in different areas of the cell wall and was found independently in various cells. The modified areas included as well plasmodesmata. The above observations enable us to presume that the cells of necrotic centre and seminecrotic zone are the place of replication and assembling of viruses and is responsible for the mechanical localization of virus infection.

  6. A Phaseolus vulgaris NADPH oxidase gene is required for root infection by Rhizobia.

    Science.gov (United States)

    Montiel, Jesús; Nava, Noreide; Cárdenas, Luis; Sánchez-López, Rosana; Arthikala, Manoj-Kumar; Santana, Olivia; Sánchez, Federico; Quinto, Carmen

    2012-10-01

    Plant NADPH oxidases [respiratory burst oxidase homologs (RBOHs)] have emerged as key players in the regulation of plant-pathogen interactions. Nonetheless, their role in mutualistic associations, such as the rhizobia-legume symbiosis, is poorly understood. In this work, nine members of the Phaseolus vulgaris Rboh gene family were identified. The transcript of one of these, PvRbohB, accumulated abundantly in shoots, roots and nodules. PvRbohB promoter activity was detected in meristematic regions of P. vulgaris roots, as well as during infection thread (IT) progression and nodule development. RNA interference (RNAi)-mediated PvRbohB down-regulation in transgenic roots reduced reactive oxygen species (ROS) production and lateral root density, and greatly impaired nodulation. Microscopy analysis revealed that progression of the ITs was impeded at the base of root hairs in PvRbohB-RNAi roots. Furthermore, the few nodules that formed in PvRbohB-down-regulated roots displayed abnormally wide ITs and reduced nitrogen fixation. These findings indicate that this common bean NADPH oxidase is crucial for successful rhizobial colonization and probably maintains proper IT growth and shape.

  7. Study on mutation induced effect of gamma ray and DES on black bean phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Le Thi Dinh; Pham Le Ha; Nguyen Van Toan; Le Xuan Tham [Nuclear Research Institute, Radiobiology Department, Dalat (Viet Nam)

    2001-03-01

    The study on mutation induced effect of gamma ray and DES on black bean Phaseolus vulgaris was carried out at Radiobiology Department, Nuclear Research Institute of Dalat. Dry seeds of variety No.1847 - Bonita - Cuba in set of 13 black bean varieties were irradiated with gamma ray from {sup 60}Co source at dose range from 150 Gy to 350 Gy and treated with DES at concentration from 0.1% to 0.3% in 2 hours for experiments in laboratory. The doses of 200, 250, 300 Gy and concentration of 0.2% DES in 2 hours were selected to treat dry seeds for experiments on the field. In populations of M{sub 1} generation, the height, number of branches and fruits per plant, number of seeds per fruit were decreased with increasing of irradiation doses. In populations of M{sub 2} generation, individual variants in leaf shape, chlorophyll, short stem, dwarf, early maturity, flowering in very short time were obtained and selected in all treatment cases. Mutation frequency at dose of 300 Gy was higher than that in other treatment cases, but ratio of sterility is also largest. The mutant lines of early maturity and short stem with flowering in very short time are promised materials for further studies. (author)

  8. Catalytic and structural diversity of the fluazifop-inducible glutathione transferases from Phaseolus vulgaris.

    Science.gov (United States)

    Chronopoulou, Evangelia; Madesis, Panagiotis; Asimakopoulou, Basiliki; Platis, Dimitrios; Tsaftaris, Athanasios; Labrou, Nikolaos E

    2012-06-01

    Plant glutathione transferases (GSTs) comprise a large family of inducible enzymes that play important roles in stress tolerance and herbicide detoxification. Treatment of Phaseolus vulgaris leaves with the aryloxyphenoxypropionic herbicide fluazifop-p-butyl resulted in induction of GST activities. Three inducible GST isoenzymes were identified and separated by affinity chromatography. Their full-length cDNAs with complete open reading frame were isolated using RACE-RT and information from N-terminal amino acid sequences. Analysis of the cDNA clones showed that the deduced amino acid sequences share high homology with GSTs that belong to phi and tau classes. The three isoenzymes were expressed in E. coli and their substrate specificity was determined towards 20 different substrates. The results showed that the fluazifop-inducible glutathione transferases from P. vulgaris (PvGSTs) catalyze a broad range of reactions and exhibit quite varied substrate specificity. Molecular modeling and structural analysis was used to identify key structural characteristics and to provide insights into the substrate specificity and the catalytic mechanism of these enzymes. These results provide new insights into catalytic and structural diversity of GSTs and the detoxifying mechanism used by P. vulgaris.

  9. Identification and analysis of alternative splicing events in Phaseolus vulgaris and Glycine max.

    Science.gov (United States)

    Iñiguez, Luis P; Ramírez, Mario; Barbazuk, William B; Hernández, Georgina

    2017-08-22

    The vast diversification of proteins in eukaryotic cells has been related with multiple transcript isoforms from a single gene that result in alternative splicing (AS) of primary transcripts. Analysis of RNA sequencing data from expressed sequence tags and next generation RNA sequencing has been crucial for AS identification and genome-wide AS studies. For the identification of AS events from the related legume species Phaseolus vulgaris and Glycine max, 157 and 88 publicly available RNA-seq libraries, respectively, were analyzed. We identified 85,570 AS events from P. vulgaris in 72% of expressed genes and 134,316 AS events in 70% of expressed genes from G. max. These were categorized in seven AS event types with intron retention being the most abundant followed by alternative acceptor and alternative donor, representing ~75% of all AS events in both plants. Conservation of AS events in homologous genes between the two species was analyzed where an overrepresentation of AS affecting 5'UTR regions was observed for certain types of AS events. The conservation of AS events was experimentally validated for 8 selected genes, through RT-PCR analysis. The different types of AS events also varied by relative position in the genes. The results were consistent in both species. The identification and analysis of AS events are first steps to understand their biological relevance. The results presented here from two related legume species reveal high conservation, over ~15-20 MY of divergence, and may point to the biological relevance of AS.

  10. Green manure and its influence on bean (Phaseolus vulgaris L. in agroecological system

    Directory of Open Access Journals (Sweden)

    Marisol Rivero Herrada

    2016-07-01

    Full Text Available Green manures have been used as biomass producers and suppliers of nutrients and keep the soil productive potential in tropical regions. The objective of this study was to evaluate the production of dry biomass and nutrient concentration and accumulation in plants of green manure in two cropping systems, without associating and associated with millet and its influence on the nutritional status of the bean (Phaseolus vulgaris L. in succession in agroecological production. Four legumes (Canavalia ensiformis Adans; Cajanus cajan (L. Millsp.; Crotalaria juncea L.; Mucuna pruriens (L. DC and one grass (Pennisetum glaucum L. as association plant, were evaluated. A randomized block design was used, with eight treatments and four repetitions. The green manure plants were cut and left on the ground at 60 days after planting and bean was planted 20 days after cutting the manure plants. The evaluated variables were dried biomass production, nutrient content of green manure and nutrient content in leaves of beans in succession. The production of dry biomass of green manure was higher than 9.00 t ha-1. Mucuna stood out with the greatest tenors of N, Ca, Mg, pork beans with the highest tenors of K, Cu, Mn. The greatest accumulation of P, K and Ca nutrients was in pigeon pea. The highest values of N and Mg were obtained in mucuna. Higher C / N relation was obtained in rotalaria.

  11. Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)

    Science.gov (United States)

    Cai, X.; Henry, R. L.; Takemoto, L. J.; Guikema, J. A.; Wong, P. P.; Spooner, B. S. (Principal Investigator)

    1992-01-01

    The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.

  12. Nutritional traits of bean ( Phaseolus vulgaris ) seeds from plants chronically exposed to ozone pollution.

    Science.gov (United States)

    Iriti, Marcello; Di Maro, Antimo; Bernasconi, Silvana; Burlini, Nedda; Simonetti, Paolo; Picchi, Valentina; Panigada, Cinzia; Gerosa, Giacomo; Parente, Augusto; Faoro, Franco

    2009-01-14

    The effect of chronic exposure to ozone pollution on nutritional traits of bean ( Phaseolus vulgaris L. cv. Borlotto Nano Lingua di Fuoco) seeds from plants grown in filtered and nonfiltered open-top chambers (OTCs) has been investigated. Results showed that, among seed macronutrients, ozone significantly raised total lipids, crude proteins, and dietary fiber and slightly decreased total free amino acid content, although with a significant reduction of asparagine, lysine, valine, methionine, and glycine, compensated by a conspicuous augmentation of ornithine and tryptophan. Phytosterol analysis showed a marked increase of beta-sitosterol, stigmasterol, and campesterol in seeds collected from nonfiltered OTCs. With regard to secondary metabolites, ozone exposure induced a slight increase of total polyphenol content, although causing a significant reduction of some flavonols (aglycone kaempferol and its 3-glucoside derivative) and hydroxycinnamates (caffeic, p-coumaric, and sinapic acids). Total anthocyanins decreased significantly, too. Nevertheless, ozone-exposed seeds showed higher antioxidant activity, with higher Trolox equivalent antioxidant capacity (TEAC) values than those measured in seeds collected from filtered air.

  13. Phytotoxic Effect of Landfill and Leachate Pollution Indexes on Germination and Seedling of Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Márquez-Benavides Liliana

    2014-08-01

    Full Text Available Appropriate solid waste management includes leachate management, an effluent that results from the degradation of solid waste, moisture content and pluvial additions to the disposal site. Due to poor management of the landfill, sometimes leachate is likely to reach nearby areas, affecting soil water and vegetal area. A powerful tool to assess the pollution potential of a given leachate is the leachate pollution index (LPI developed by Kummar & Alappat (2005 that evaluates 18 parameters in order to calculate a value between 5-100 being 100 the highest in pollution potential. The LPI allows the comparison between leachates from different sites and ages, and also assists in the decision making process on leachate treatment. However, it is currently unknown if this value can also be related to the fitotóxico effect of a leachate on Phaseolus vulgaris L. The aim of this work was to calculate the LPI of two leachates and compare the effect on P. vulgaris L (common bean. A greenhouse scale experiment was set up, the studied variables were seed germination per cent (% and phenotype of P. vulgaris at seedling step after treated with several leachate concentrations from Guanajuato (GTO and Toluca (TOL, México. Results showed that a greater LPI (34.8 from GTO did not correspond to a largest fitotoxic effect on P. vulgaris. This bioassay could be a completely tool with LPI to evaluate pollution potential of leachate approaching to normal environmental conditions.

  14. Extended map for the phaseolin linkage group ofPhaseolus vulgaris L.

    Science.gov (United States)

    Vallejos, C E; Chase, C D

    1991-09-01

    The linkage relationship of 11 bean (Phaseolus vulgaris) seed proteins (including phaseolin), 9 enzyme loci, and theP locus were analyzed in backcross and F2 progenies by use of the software package "Mapmaker." The progenies were obtained by crossing the breeding line 'XR-235-1' and the cultivar 'Calima'. Allelic differences for seed protein loci were detected with SDS-PAGE and those for enzyme loci with starch gel electrophoresis and activity stains. The seed coat color of 'Calima' is a red/beige mottled pattern and that of 'XR-235-1' is white. Segregation at theP locus was followed by recording the phenotype of the BC1S1 and F3 seed. A linkage group comprising ca. 90 cM was detected with the following gene order:Est-2 - 11 -Pha - 8 - (Spe/Spg) - 24 - P - 9 - (Spa/Spv) - 16 -Spba - 22 -Mdh-1. In addition, another linkage group was detected: (Spd/Spf/Sph) - 5 -Spca. Therefore, the seed proteins appear to be organized in clusters in the bean genome.

  15. Growth and mitochondrial respiration of mungbeans (Phaseolus aureus Roxb.) germinated at low pressure

    Science.gov (United States)

    Musgrave, M. E.; Gerth, W. A.; Scheld, H. W.; Strain, B. R.

    1988-01-01

    Mungbean (Phaseolus aureus Roxb.) seedlings were grown hypobarically to assess the effects of low pressure (21-24 kilopascals) on growth and mitochondrial respiration. Control seedlings grown at ambient pressure (101 kilopascals) were provided amounts of O2 equivalent to those provided experimental seedlings at reduced pressure to factor out responses to O2 concentration and to total pressure. Respiration was assayed using washed mitochondria, and was found to respond only to O2 concentration. Regardless of total pressure, seedlings grown at 2 millimoles O2 per liter had higher state 3 respiration rates and decreased percentages of alternative respiration compared to ambient (8.4 millimoles O2 per liter) controls. In contrast, seedling growth responded to total pressure but not to O2 concentration. Seedlings were significantly larger when grown under low pressure. While low O2 (2 millimoles O2 per liter) diminished growth at ambient pressure, growth at low pressure in the same oxygen concentration was enhanced. Respiratory development and growth of mungbean seedlings under low pressure is unimpaired whether oxygen or air is used as the chamber gas, and further, low pressure can improve growth under conditions of poor aeration.

  16. Functional properties of proteins from Lima bean (Phaseolus lunatus L.) seeds.

    Science.gov (United States)

    Chel-Guerrero, L; Gallegos-Tintoré, S; Martínez-Ayala, A; Castellanos-Ruelas, A; Betancur-Ancona, D

    2011-04-01

    Functional properties were identified for the total globulin (TG), 7S and 11S fractions of Lima bean (Phaseolus lunatus L.) seeds. The 11S component accounted for 58.3% of TGs and the 7S for 41.7%. Solubility was higher in the 7S fraction, especially at alkaline pHs. Water-holding capacity was similar (3 g water/g sample) in both globulin fractions. Oil-holding capacity was higher in the 11S fraction, which also exhibited better foaming capacity and foam stability than the 7S and TG fractions at alkaline pHs. The TG and 7S fractions exhibited low emulsifying capacity and emulsion stability at different pHs (5, 7 and 9), but the 11S fraction had relatively higher values. In suspension at low concentrations, all fractions exhibited shear-thinning (pseudoplastic) behavior. The studied Lima bean globulin fractions exhibit functional properties which make them potentially apt for use in some industrial food systems.

  17. Iron and zinc retention in common beans (Phaseolus vulgaris L. after home cooking

    Directory of Open Access Journals (Sweden)

    Lucia M. J. Carvalho

    2012-02-01

    Full Text Available Background : According to the World Health Organization (WHO, iron, iodine, and Vitamin A deficiencies are the most common forms of malnutrition, leading to severe public health consequences. The importance of iron and zinc in human nutrition and the number of children found to be deficient in these nutrients make further studies on retention in cooked grains and cooked bean broth important. Objectives : This work aimed to evaluate iron and zinc retention in six common bean (Phaseolus vulgaris L. cultivars under the following conditions: raw beans, regular pot cooking, pressure cooking, with and without previous water soaking, and broth. Design : Determination of iron and zinc content in the raw, cooked bean grains and broth samples was carried out by Inductively Coupled Plasma (ICP Optical Emission Spectrometry (Spectro Analytical Instrument – Spectroflame P. All experiments and analyses were carried out in triplicate. Results : Overall, regardless of the cooking method, with or without previous water soaking, the highest zinc concentration was found in the cooked bean grains. However, pressure cooking and previous water soaking diminished iron retention in the cooked grains, while increasing it in the bean broth. Conclusion : The common bean was confirmed to be an excellent source of iron and zinc for human consumption, and it was suggested that beans should be consumed in a combined form, i.e. grain with bean broth.

  18. Stress induced acquisition of somatic embryogenesis in common bean Phaseolus vulgaris L.

    Science.gov (United States)

    Cabrera-Ponce, José Luis; López, Liliana; León-Ramírez, Claudia G; Jofre-Garfias, Alba E; Verver-y-Vargas, Aurora

    2015-03-01

    Common bean Phaseolus vulgaris L. has been shown to be a recalcitrant plant to induce somatic embryogenesis (SE) under in vitro conditions. We used an alternative strategy to induce SE in common bean based upon the use of a cytokinin (BAP) coupled with osmotic stress adaptation instead of SE response that is induced by auxins. Explants derived from zygotic embryos of common bean were subjected to osmotic stress (sucrose 12 % w/v, 0.5 M) in the presence of BAP 10 mg/L and adenine free base 40 mg/L to induce somatic embryos from specific competent cells of the apical meristem and cotyledonary node. Somatic embryos were obtained from the competent cells in a direct response (direct SE). In a secondary response (secondary SE), those somatic embryos formed proembryogenic masses (PEM) that originated/developed into secondary somatic embryos and showed the SE ontogeny. Maturation of somatic embryos was achieved by using different osmolality media and converted to plants. Full-visible light spectrum was necessary to achieve efficient plant regeneration. Long-term recurrent SE was demonstrated by propagation of PEM at early stages of SE. This protocol is currently being applied for stable genetic transformation by means of Agrobacterium tumefaciens and bioballistics as well as for basic biochemical and molecular biology experiments.

  19. Molecular polymorphism related to flowering trait variation in a Phaseolus vulgaris L. collection.

    Science.gov (United States)

    Raggi, Lorenzo; Tissi, Carlo; Mazzucato, Andrea; Negri, Valeria

    2014-02-01

    The aim of this study was to investigate the flowering variation and the molecular polymorphism in key regulatory genes that control flowering in a Phaseolus vulgaris L. collection of 94 accessions from Europe and the Americas. The analysis of variance revealed that the difference in days-to-flowering between accessions was significant, with European accessions characterized by flowering precocity. Population structure analysis corroborated previous data on the genetic distinction between the Andean and Mesoamerican gene pools. A low level of admixture was detected. Genomic sequences of 15 gene fragments were obtained. About 7.0 kb per accession were sequenced and a total of 48 nucleotide substitutions identified. A Mixed Linear Model analysis, including population structure and kinship, was used to identify marker-trait associations. Haplotype tagging single nucleotide polymorphisms (htSNPs) associated with the studied traits were detected: in PvVRN1 and PvPHYB with days-to-flowering, in PvMYB29 with number of flower buds per inflorescence and in PvTFL1z and PvFCA with inflorescence length. The two genes associated with days-to-flowering control belong to the photoperiod and vernalization pathways. In particular, the PvVRN1 gene appears to play an important role in regulating the adaptation process of common bean. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  20. Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Nanjareddy, Kalpana; Blanco, Lourdes; Arthikala, Manoj-Kumar; Affantrange, Xochitl Alvarado; Sánchez, Federico; Lara, Miguel

    2014-03-01

    Nitrogen-limited conditions are considered to be a prerequisite for legume-rhizobial symbiosis, but the effects of nitrate-rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial (Rhizobim tropici) and arbusclar mycorrhizal (Glomus intraradices) symbiosis in Phaseolus vulgaris L. cv. Negro Jamapa under nitrate pre-incubation and continuous nitrate conditions. Our results indicate that nitrate pre-incubation, independent of the concentration, did not affect nodule development. However, the continuous supply of nitrate at high concentrations impaired nodule maturation and nodule numbers. Low nitrate conditions, in addition to positively regulating nodule number, biomass, and nitrogenase activity, also extended the span of nitrogen-fixing activity. By contrast, for arbuscular mycorrhizae, continuous 10 and 50 mmol/L nitrate increased the percent root length colonization, concomitantly reduced arbuscule size, and enhanced ammonia transport without affecting phosphate transport. Therefore, in this manuscript, we have proposed the importance of nitrate as a positive regulator in promoting both rhizobial and mycorrhizal symbiosis in the common bean. © 2014 Institute of Botany, Chinese Academy of Sciences.

  1. Leaf orientation and distribution in a Phaseolus vulgaris L. crop and their relation to light microclimate

    Science.gov (United States)

    Barradas, V. L.; Jones, H. G.; Clark, Jerry A.

    Changes in canopy structure parameters (leaflet orientation, leaflet inclination and leaf area index) were measured in crops of beans (Phaseolus vulgaris L.) in the field as the canopy developed between July and October. These changes were compared with the corresponding changes in seasonal light transmission. The beans showed clear heliotropic behaviour, with preferential orientation of leaflets towards the sun's beam, especially on sunny days. Nevertheless a significant proportion of the leaves pointed in other directions, with as much as 20% oriented towards the north. The highest proportion of leaf inclinations was in the range 30-40° on cloudy days and between 40° and 50° on sunny days. Two methods were compared for assessing changes in light transmission: (a) the use of a Sunfleck Ceptometer and (b) the use of continuous records obtained with sensors installed in the canopy. Over the growth period studied, the total of the leaf plus stem area indices (LS) increased from 0.26 to 5.2 with the transmission coefficient (τ) for photosynthetic photon flux density (PPFD), obtained using the Ceptometer, correspondingly decreasing from 0.72 to 0.05, and the canopy extinction coefficient decreasing from 1.4 to 0.62. The continuous records of light transmission gave generally similar estimates of τ. Some contrasting leaf angle distribution functions were compared for estimation of LS from the light measurements. The best leaf angle function to predict LS from the observed light transmission was a conical function corrected by the degree of heliotropism.

  2. Response of Phaseolus vulgaris L. to differing ozone regimes having identical total exposure

    Science.gov (United States)

    Musselman, Robert C.; Younglove, Theodore; McCool, Patrick M.

    Protocols were designed to test for differences in response of plants to ozone treatments having equal total exposure (concentration × time) but different exposure profiles Kidney beans ( Phaseolus vulgaris L., cv. California Dark Red) were exposed to ozone in controlled fumigation chambers within a greenhouse Four different ozone exposure profiles were used, each having the same total cumulative exposure (SUM00) and the same 7, 12 and 24 h seasonal means. The three exposure profiles which incorporated peak concentrations more severely impacted response parameters compared to a steady-state profile which did not exceed the National Ambient Air Quality Standard. Significant differences were found in percent necrotic leaf area, number of pods and top dry weight between exposure profiles. In additional analyses, the response parameters were regressed against seasonal cumulative ozone concentrations raised to powers of 0.33 and from 0.5 to 4 in steps of 0.5 in order to increase effective weighting of the higher concentrations. Total dry weight and leaf necrosis were best fit with the sum of the squared concentrations ( n = 2) while number of pods was best fit by the summed concentrations to the 3.5 power ( n = 3.5). These analyses suggest the peak ozone concentrations are important in determining plant response.

  3. Demonstrating a Nutritional Advantage to the Fast-Cooking Dry Bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Wiesinger, Jason A; Cichy, Karen A; Glahn, Raymond P; Grusak, Michael A; Brick, Mark A; Thompson, Henry J; Tako, Elad

    2016-11-16

    Dry beans (Phaseolus vulgaris L.) are a nutrient-dense food rich in protein and micronutrients. Despite their nutritional benefits, long cooking times limit the consumption of dry beans worldwide, especially in nations where fuelwood for cooking is often expensive or scarce. This study evaluated the nutritive value of 12 dry edible bean lines that vary for cooking time (20-89 min) from four market classes (yellow, cranberry, light red kidney, and red mottled) of economic importance in bean-consuming regions of Africa and the Americas. When compared to their slower cooking counterparts within each market class, fast-cooking dry beans retain more protein and minerals while maintaining similar starch and fiber densities when fully cooked. For example, some of the highest protein and mineral retention values were measured in the fast-cooking yellow bean cultivar Cebo Cela, which offered 20% more protein, 10% more iron, and 10% more zinc with each serving when compared with Canario, a slow-cooking yellow bean that requires twice the cooking time to become palatable. A Caco-2 cell culture model also revealed the bioavailability of iron is significantly higher in faster cooking entries (r = -0.537, P = 0.009) as compared to slower cooking entries in the same market class. These findings suggest that fast-cooking bean varieties have improved nutritive value through greater nutrient retention and improved iron bioavailability.

  4. Soybean rust resistance sources and inheritance in the common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Souza, T L P O; Dessaune, S N; Moreira, M A; Barros, E G

    2014-07-25

    Soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi, has been reported in common bean (Phaseolus vulgaris L.) cultivars and elite lines that were infected under controlled and natural field conditions in South Africa, the United States, Argentina, and Brazil. Although SBR is currently not a top priority problem for the common bean crop, many bean breeders are concerned about this disease because of the high severity and virulence diversity of P. pachyrhizi and its broad host range. In this study, a set of 44 P. vulgaris genotypes were tested for resistance to P. pachyrhizi; these genotypes included resistance sources to several fungal common bean diseases, carioca-, black- and red-seeded Brazilian cultivars, and elite lines that were developed by the main common bean breeding programs in Brazil. Twenty-four SBR resistance sources were identified. They presented the reddish-brown (RB) lesion type, characterizing resistance reactions. In addition to the RB lesion type, the PI181996 line presented the lowest disease severity mean score, considering its associated standard error value. For this reason, it was crossed with susceptible lines to study the inheritance of resistance. The results support the hypothesis that resistance to SBR in PI181996 is monogenic and dominant. We propose that this SBR resistance gene, the first to be identified and characterized in common bean, might be designated as Pkp-1.

  5. Genetic assessment of common bean (Phaseolus vulgaris L.) accessions by peroxidase gene-based markers.

    Science.gov (United States)

    Nemli, Seda; Kaya, Hilal Betul; Tanyolac, Bahattin

    2014-06-01

    Peroxidase, a plant-specific oxidoreductase, is a heme-containing glycoprotein encoded by a large multigenic family in plants. Plant peroxidases (POXs, EC 1.11.1.7) play important roles in many self-defense interactions in plants. Here, 67 common bean (Phaseolus vulgaris L.) genotypes were studied using a POX gene-based marker method. Comparison of POX genes could resolve evolutionary relationships in common bean. Eighty fragments were obtained with 20 primer pairs that amplified one (POX8c) to eight (ATP29) bands, with a mean of four bands per primer pair. The average (polymorphic information content) PIC value for the POX products was 0.40. The maximum variation (93%) was found between Turkey (#33) and India (#52) and between Antalya (#33) and India (#53). The minimum variation (0%) was found among four pairs: Bozdag (#2) and Karadeniz (#38), Kirklareli (#11) and Turkey (#15, 16, 43), Bandirma (#13) and Turkey (#15, 16, 43), and Kirklareli (#10) and Bandirma (#22). UPGMA was used to discriminate the common bean genotypes into five clusters, while STRUCTURE software was used to investigate the genetic population structure. The results showed that POX gene family markers can be used to study genotypic diversity and provide new information for breeding programs and common bean improvement practices. © 2013 Society of Chemical Industry.

  6. Antipeptide antibodies that can distinguish specific subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.)

    Science.gov (United States)

    Cai, X.; Henry, R. L.; Takemoto, L. J.; Guikema, J. A.; Wong, P. P.; Spooner, B. S. (Principal Investigator)

    1992-01-01

    The amino acid sequences of the beta and gamma subunit polypeptides of glutamine synthetase from bean (Phaseolus vulgaris L.) root nodules are very similar. However, there are small regions within the sequences that are significantly different between the two polypeptides. The sequences between amino acids 2 and 9 and between 264 and 274 are examples. Three peptides (gamma 2-9, gamma 264-274, and beta 264-274) corresponding to these sequences were synthesized. Antibodies against these peptides were raised in rabbits and purified with corresponding peptide-Sepharose affinity chromatography. Western blot analysis of polyacrylamide gel electrophoresis of bean nodule proteins demonstrated that the anti-beta 264-274 antibodies reacted specifically with the beta polypeptide and the anti-gamma 264-274 and anti-gamma 2-9 antibodies reacted specifically with the gamma polypeptide of the native and denatured glutamine synthetase. These results showed the feasibility of using synthetic peptides in developing antibodies that are capable of distinguishing proteins with similar primary structures.

  7. Diallel analysis to choose parents for black bean (Phaseolus vulgaris L.) breeding.

    Science.gov (United States)

    Moura, L M; Carneiro, P C S; Vale, N M; Barili, L D; Silva, L C; Carneiro, J E S; Cruz, C D

    2016-08-29

    In this study, conducted in two different seasons, we aimed to choose parents to obtain promising segregating populations for the extraction of black bean (Phaseolus vulgaris L.) lines that are superior in terms of disease resistance, plant architecture, and grain yield. Twelve parents were arranged in two groups to compose a partial diallel in a 5 x 7 scheme. Group 1 was composed of parents with black grains and erect plant architecture, while group 2 was composed of parents that had carioca grains and were resistant to the main fungal diseases that occur in the common bean. The following traits were evaluated: severity of angular leaf spot (ALS), plant architecture (PAG), and grain yield (YIELD). The data were analyzed according to a partial diallel model using parents and F1 hybrids. In the genetic control of ALS and PAG, additive effects were predominant, while for YIELD, additive effects were predominant in one season and dominance effects were in another season, because it is a more complex trait than ALS and PAG. For YIELD, we observed an interaction between general combining ability and specific combining ability between seasons. The genes that control ALS, PAG, and YIELD were in eight of the 12 parents evaluated in the diallel. The cultivar 'BRS Estilo' is suitable to use as a parent in common bean breeding in terms of ALS, PAG and YIELD. Recurrent selection is the most recommended option for simultaneously breeding for PAG, YIELD, and resistance to angular leaf spot in bean culture.

  8. Environmental Effects of Nanoceria on Seed Production of Common Bean (Phaseolus vulgaris): A Proteomic Analysis.

    Science.gov (United States)

    Majumdar, Sanghamitra; Almeida, Igor C; Arigi, Emma A; Choi, Hyungwon; VerBerkmoes, Nathan C; Trujillo-Reyes, Jesica; Flores-Margez, Juan P; White, Jason C; Peralta-Videa, Jose R; Gardea-Torresdey, Jorge L

    2015-11-17

    The rapidly growing literature on the response of edible plants to nanoceria has provided evidence of its uptake and bioaccumulation, which delineates a possible route of entry into the food chain. However, little is known about how the residing organic matter in soil may affect the bioavailability and resulting impacts of nanoceria on plants. Here, we examined the effect of nanoceria exposure (62.5-500 mg/kg) on kidney bean (Phaseolus vulgaris) productivity and seed quality as a function of soil organic matter content. Cerium accumulation in the seeds produced from plants in organic matter enriched soil showed a dose-dependent increase, unlike in low organic matter soil treatments. Seeds obtained upon nanoceria exposure in soils with higher organic matter were more susceptible to changes in nutrient quality. A quantitative proteomic analysis of the seeds produced upon nanoceria exposure provided evidence for upregulation of stress-related proteins at 62.5 and 125 mg/kg nanoceria treatments. Although the plants did not exhibit overt toxicity, the major seed proteins primarily associated with nutrient storage (phaseolin) and carbohydrate metabolism (lectins) were significantly down-regulated in a dose dependent manner upon nanoceria exposure. This study thus suggests that nanoceria exposures may negatively affect the nutritional quality of kidney beans at the cellular and molecular level. More confirmatory studies with nanoceria along different species using alternative and orthogonal "omic" tools are currently under active investigation, which will enable the identification of biomarkers of exposure and susceptibility.

  9. The plant nitrogen mobilization promoted by Colletotrichum lindemuthianum in Phaseolus leaves depends on fungus pathogenicity.

    Science.gov (United States)

    Tavernier, Virginie; Cadiou, Sandrine; Pageau, Karine; Laugé, Richard; Reisdorf-Cren, Michèle; Langin, Thierry; Masclaux-Daubresse, Céline

    2007-01-01

    Nitrogen plays an essential role in the nutrient relationship between plants and pathogens. Some studies report that the nitrogen-mobilizing plant metabolism that occurs during abiotic and biotic stress could be a 'slash-and-burn' defence strategy. In order to study nitrogen recycling and mobilization in host plants during pathogen attack and invasion, the Colletotrichum lindemuthianum/Phaseolus vulgaris interaction was used as a model. C. lindemuthianum is a hemibiotroph that causes anthracnose disease on P. vulgaris. Non-pathogenic mutants and the pathogenic wild-type strain were used to compare their effects on plant metabolism. The deleterious effects of infection were monitored by measuring changes in chlorophyll, protein, and amino acid concentrations. It was shown that amino acid composition changed depending on the plant-fungus interaction and that glutamine accumulated mainly in the leaves infected by the pathogenic strain. Glutamine accumulation correlated with the accumulation of cytosolic glutamine synthetase (GS1 alpha) mRNA. The most striking result was that the GS1 alpha gene was induced in all the fungus-infected leaves, independent of the strain used for inoculation, and that GS1 alpha expression paralleled the PAL3 and CHS defence gene expression. It is concluded that a role of GS1 alpha in plant defence has to be considered.

  10. Elicitor-mediated induction of chalcone isomerase in Phaseolus vulgaris cell suspension cultures.

    Science.gov (United States)

    Dixon, R A; Gerrish, C; Lamb, C J; Robbins, M P

    1983-12-01

    Approximately fourfold increases in the extractable activity of the enzyme chalcone isomerase (CHI, EC 5.5.1.6) were observed within 24 h of treatment of cell suspension cultures of Phaseolus vulgaris with a crude elicitor preparation heatreleased from the cell walls of the bean pathogen Colletotrichum lindemuthianum. The induction of CHI activity was highly dependent upon elicitor concentration, with maximum induction occurring in two discrete concentration ranges. A basal half-life for CHI>32 h in control cultures was determined by labelling with (2)H from (2)H2O followed by analysis of the equilibrium distribution of enzyme activity in CsCl density gradients. Comparative density labelling indicated that at both the lower and higher effective elicitor concentrations, the induced appearance of CHI activity was the result of an apparent initial activation of pre-existing enzyme followed by an increase in the rate of de-novo synthesis of the enzyme as compared with non-elicited controls. The increased appearance of the enzyme over the first 8 h in elicitor-treated cultures was inhibited by cycloheximide, cordycepin and actinomycin D. The results are discussed in relation to the mechanisms of co-ordinate enzyme induction operating in French-bean cell cultures exposed to fungal elicitors.

  11. Elicitor-induced prolyl hydroxylase from French bean (Phaseolus vulgaris). Localization, purification and properties.

    Science.gov (United States)

    Bolwell, G P; Robbins, M P; Dixon, R A

    1985-08-01

    The enzyme prolyl hydroxylase (proline: 2-oxoglutarate dioxygenase, EC 1.14.11.12), induced in suspension-cultured cells of Phaseolus vulgaris L. (French bean) by treatment with an elicitor preparation from the phytopathogenic fungus Colletotrichum lindemuthianum, has been investigated. The enzyme, which catalyses the hydroxylation of poly-L-proline with the stoichiometric decarboxylation of 2-oxoglutarate, has been shown to be localized mainly in smooth endoplasmic reticulum. After solubilization from microsomal membranes, the hydroxylase was purified by ion-exchange chromatography and affinity chromatography on poly-L-proline-Sepharose 4B. The subunit Mr, as assessed by sodium dodecyl sulphate/poly-acrylamide-gel electrophoresis, was 65 000, the subunit apparently being recovered as a doublet: the subunits associate under non-denaturing conditions to give at least a tetramer. The bean hydroxylase has kinetic properties and cofactor requirements similar to those previously reported for the enzyme from other plants. Elicitor treatment of suspension-cultured bean cells leads to a rapid induction of prolyl hydroxylase activity concomitant with induction of a protein: arabinosyl-transferase and increased levels of an arabinosylated hydroxyproline-rich protein.

  12. Purification and Characterization of a Polygalacturonase-Inhibiting Protein from Phaseolus vulgaris L.

    Science.gov (United States)

    Cervone, F; De Lorenzo, G; Degrà, L; Salvi, G; Bergami, M

    1987-11-01

    Homogeneous endo-polygalacturonase (PG) was covalently bound to cyanogen-bromide-activated Sepharose, and the resulting PG-Sepharose conjugate was utilized to purify, by affinity chromatography, a protein from Phaseolus vulgaris hypocotyls that binds to and inhibits PG. Isoelectric focusing of the purified PG-inhibiting protein (PGIP) showed a major protein band that coincided with PG-inhibiting activity. PGIP formed a complex with PG at pH 5.0 and at low salt concentrations. The complex dissociated in 0.5 m Na-acetate and pH values lower than 4.5 or higher than 6.0. Formation of the PG-PGIP complex resulted in complete inhibition of PG activity. PG activity was restored upon dissociation of the complex. The protein exhibited inhibitory activity toward PGs from Colletotrichum lindemuthianum, Fusarium moniliforme and Aspergillus niger. The possible role of PGIP in regulating the activity of fungal PG's and their ability to elicit plant defense reactions are discussed.

  13. Purification and Characterization of a Polygalacturonase-Inhibiting Protein from Phaseolus vulgaris L. 1

    Science.gov (United States)

    Cervone, Felice; De Lorenzo, Giulia; Degrà, Luisa; Salvi, Giovanni; Bergami, Mario

    1987-01-01

    Homogeneous endo-polygalacturonase (PG) was covalently bound to cyanogen-bromide-activated Sepharose, and the resulting PG-Sepharose conjugate was utilized to purify, by affinity chromatography, a protein from Phaseolus vulgaris hypocotyls that binds to and inhibits PG. Isoelectric focusing of the purified PG-inhibiting protein (PGIP) showed a major protein band that coincided with PG-inhibiting activity. PGIP formed a complex with PG at pH 5.0 and at low salt concentrations. The complex dissociated in 0.5 m Na-acetate and pH values lower than 4.5 or higher than 6.0. Formation of the PG-PGIP complex resulted in complete inhibition of PG activity. PG activity was restored upon dissociation of the complex. The protein exhibited inhibitory activity toward PGs from Colletotrichum lindemuthianum, Fusarium moniliforme and Aspergillus niger. The possible role of PGIP in regulating the activity of fungal PG's and their ability to elicit plant defense reactions are discussed. Images Fig. 3 PMID:16665751

  14. Evaluation of the oxidative burst in suspension cell culture of Phaseolus vulgaris.

    Science.gov (United States)

    Janisch, Kerstin; Schempp, Harald

    2004-01-01

    Plants respond to the attack of pathogens with the oxidative burst, a production of reactive oxygen species (ROS). In this work a cell culture suspension of Phaseolus vulgaris was used to investigate the oxidative burst triggered by a conidia suspension of different races of Colletotrichum lindemuthianum. As a defence response of the cells a two-phase peak was observed with all used races of Colletotrichum lindemuthianum, varying only in the produced amounts of hydrogen peroxide. Findings with additives such as superoxide dismutase (SOD), diphenyleneiodonium (DPI) and catalase gave rise to the conclusion that more superoxide radicals were produced than be detectable with Amplex Red as hydrogen peroxide. It is assumed that the conversion of the superoxide radical is spontaneous and not driven via a cell-derived superoxide dismutase. The addition of low-molecular cell wall components (ergosterol, glucosamine, galactosamine) showed clearly that compounds like this act as elicitors and thus are involved in triggering the burst. Furthermore, an evaluation of the metabolizing capacities of hydrogen peroxide of the suspension culture cells revealed the enormous capacity of the cells to detoxify this ROS.

  15. Cyanogenesis of Wild Lima Bean (Phaseolus lunatus L.) Is an Efficient Direct Defence in Nature

    Science.gov (United States)

    Ballhorn, Daniel J.; Kautz, Stefanie; Heil, Martin; Hegeman, Adrian D.

    2009-01-01

    In natural systems plants face a plethora of antagonists and thus have evolved multiple defence strategies. Lima bean (Phaseolus lunatus L.) is a model plant for studies of inducible indirect anti-herbivore defences including the production of volatile organic compounds (VOCs) and extrafloral nectar (EFN). In contrast, studies on direct chemical defence mechanisms as crucial components of lima beans' defence syndrome under natural conditions are nonexistent. In this study, we focus on the cyanogenic potential (HCNp; concentration of cyanogenic glycosides) as a crucial parameter determining lima beans' cyanogenesis, i.e. the release of toxic hydrogen cyanide from preformed precursors. Quantitative variability of cyanogenesis in a natural population of wild lima bean in Mexico was significantly correlated with missing leaf area. Since existing correlations do not by necessity mean causal associations, the function of cyanogenesis as efficient plant defence was subsequently analysed in feeding trials. We used natural chrysomelid herbivores and clonal lima beans with known cyanogenic features produced from field-grown mother plants. We show that in addition to extensively investigated indirect defences, cyanogenesis has to be considered as an important direct defensive trait affecting lima beans' overall defence in nature. Our results indicate the general importance of analysing ‘multiple defence syndromes’ rather than single defence mechanisms in future functional analyses of plant defences. PMID:19424497

  16. Evaluation of chitooligosaccharide application on mineral accumulation and plant growth in Phaseolus vulgaris.

    Science.gov (United States)

    Chatelain, Philippe G; Pintado, Manuela E; Vasconcelos, Marta W

    2014-02-01

    Chitooligosaccharides (COS) - water soluble derivatives from chitin, are an interesting group of molecules for several biological applications, for they can enter plant cells and bind negatively charged molecules. Several studies reported an enhanced plant growth and higher crop yield due to chitosan application in soil grown plants, but no studies have looked on the effect of COS application on plant mineral nutrient dynamics in hydroponically grown plants. In this study, Phaseolus vulgaris was grown in hydroponic culture and the effect of three different concentrations of COS on plant growth and mineral accumulation was assessed. There were significant changes in mineral allocations for Mo, B, Zn, P, Pb, Cd, Mn, Fe, Mg, Ca, Cu, Na, Al and K among treatments. Plant morphology was severely affected in high doses of COS, as well as lignin concentration in the stem and the leaves, but not in the roots. Chlorophyll A, B and carotenoid concentrations did not change significantly among treatments, suggesting that even at higher concentrations, COS application did not affect photosynthetic pigment accumulation. Plants grown at high COS levels had shorter shoots and roots, suggesting that COS can be phytotoxic to the plant. The present study is the first detailed report on the effect of COS application on mineral nutrition in plants, and opens the door for future studies that aim at utilizing COS in biofortification or phytoremediation programs.

  17. A genome-wide analysis of differentiation between wild and domesticated Phaseolus vulgaris from Mesoamerica.

    Science.gov (United States)

    Papa, R; Acosta, J; Delgado-Salinas, A; Gepts, P

    2005-10-01

    Lack of introgression or divergent selection may be responsible for the maintenance of phenotypic differences between sympatric populations of crops and their wild progenitors. To distinguish between these hypotheses, amplified fragment length polymorphism markers were located on a molecular linkage map of Phaseolus vulgaris relative to genes for the domestication syndrome and other traits. Diversity for these same markers was then analyzed in two samples of wild and domesticated populations from Mesoamerica. Differentiation between wild and domesticated populations was significantly higher in parapatric and allopatric populations compared to sympatric populations. It was also significantly higher near genes for domestication compared to those away from these genes. Concurrently, the differences in genetic diversity between wild and domesticated populations were strongest around such genes. These data suggest that selection in the presence of introgression appears to be a major evolutionary factor maintaining the identity of wild and domesticated populations in sympatric situations. Furthermore, alleles from domesticated populations appear to have displaced alleles in sympatric wild populations, thus leading to a reduction in genetic diversity in such populations. These results also provide a possible experimental framework for assessing the long-term risk of transgene escape and the targeting of transgenes inside the genome to minimize the survival of these transgenes into wild populations following introduction by gene flow.

  18. US Forest Service Roadless Areas: Colorado Roadless Rule

    Data.gov (United States)

    US Forest Service, Department of Agriculture — A map service, available on the www that depicts the boundaries of Roadless Areas designated by the Colorado Roadless Rule of 2012 and managed by the US Forest...

  19. Final Critical Habitat for the Little Colorado spinedace (Lepidomeda vittata)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — provide the user with a general idea of areas where final critical habitat for Little Colorado spinedace (Lepidomeda vittata) occur based on the description provided...

  20. The Colorado Plateau coal assessment study area, 2000 (cpstdyg)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This is a coverage of the Colorado Plateau coal assessment study area. The study area outline was drawn on the county lines that most closely outline the...

  1. Colorado cultural resource survey: Management data form [5JA784

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document includes the survey forms necessary to assess cultural resources in Colorado. This document assesses the Lewis children graves (site # 5JA1478) on...

  2. Photographs of historical mining operations in Colorado and Utah

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A collection of photographs of mine sites, mining operations, and tailings taken prior to 1980 at a variety of sites throughout Colorado and Utah. A database of...

  3. Mahogany Ledge Digital Line Outcrop of the Piceance Basin, Colorado

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Mahogany ledge outcrop was needed to limit resource calculations in the Piceance Basin, Colorado as part of a 2009 National Oil Shale Assessment.

  4. Final Critical Habitat for the Colorado pikeminnow (Ptychocheilus lucius)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Colorado pikeminnow (Ptychocheilus lucius) occur based on the description provided...

  5. Colorado's hydrothermal resource base: an assessment

    Energy Technology Data Exchange (ETDEWEB)

    Pearl, R.H.

    1981-01-01

    As part of its effort to more accurately describe the nations geothrmal resource potential, the US Department of Energy/Division of Geothermal Energy contracted with the Colorado Geological survey to appraise the hydrothermal (hot water) geothermal resources of Colorado. Part of this effort required that the amount of energy that could possibly be contained in the various hydrothermal systems in Colorado be estimated. The findings of that assessment are presented. To make these estimates the geothermometer reservoir temperatures estimated by Barrett and Pearl (1978) were used. In addition, the possible reservoir size and extent were estimated and used. This assessment shows that the total energy content of the thermal systems in Colorado could range from 4.872 x 10{sup 15} BTU's to 13.2386 x 10{sup 15} BTU's.

  6. Vomiting Disorder on Rise in Weed-Friendly Colorado

    Science.gov (United States)

    ... fullstory_162895.html Vomiting Disorder on Rise in Weed-Friendly Colorado Doctors say problem may become more ... Jan. 5, 2017 (HealthDay News) -- Long-term heavy marijuana use can cause chronic vomiting and abdominal pain ...

  7. Colorado River Sewer System Joint Venture to Upgrade Wastewater System

    Science.gov (United States)

    SAN FRANCISCO -Today, the Colorado River Sewer System Joint Venture, located in Parker, Ariz. entered into an agreement with the EPA to upgrade their wastewater treatment system to meet stringent water quality standards. The cost of the upgrade is ap

  8. Mean-annual erosion potential for Colorado and New Mexico

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The U.S. Geological Survey Data Series provides raster data representing an estimate of the mean-annual erosion potential of a 30-meter raster cell in Colorado and...

  9. Colorado cultural resource survey: Management data form [5JA784

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document includes the survey forms necessary to assess cultural resources in Colorado. This document assesses the Allard Ranch (site # 5JA784, temporary #...

  10. Final Critical Habitat for the Colorado pikeminnow (Ptychocheilus lucius)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — To provide the user with a general idea of areas where final critical habitat for Colorado pikeminnow (Ptychocheilus lucius) occur based on the description provided...

  11. Final Critical Habitat for the Little Colorado spinedace (Lepidomeda vittata)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — provide the user with a general idea of areas where final critical habitat for Little Colorado spinedace (Lepidomeda vittata) occur based on the description provided...

  12. Corbiculae fluminea as a bioindicator on the Lower Colorado River

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Tissue samples from Asiatic clam (Corbimla fluminea) from the lower Colorado River were analyzed for trace element concentrations. Selenium and arsenic were elevated...

  13. Raton basin coalbed methane production picking up in Colorado

    Science.gov (United States)

    Hemborg, H. Thomas

    1996-01-01

    Coalbed methane production in the Raton basin of south-central Colorado and northeast New Mexico has gone over pilot testing and entered the development stage which is expected to last several years. The development work is restricted to roughly a 25 mile by 15 mile wide `fairway' centered about 20 miles west of Trinidad, Colorado. At last count, 85 wells were producing nearly 17.5 MMcfd of coalbed methane from the basin's Raton and Vermejo formation coals.

  14. The Colorado Plateau II : Biophysical, Socioeconomic, and Cultural Research

    OpenAIRE

    Van Riper, Charles; Mattson, David J.

    2005-01-01

    Abstract from GoogleBooks: The publication of The Colorado Plateau: Cultural, Biological, and Physical Research in 2004 marked a timely summation of current research in the Four Corners states. This new volume, derived from the seventh Biennial Conference on the Colorado Plateau in 2003, complements the previous book by again focusing on the integration of science into resource management issues. The 32 chapters range in content from measuring human impacts on cultural resources, through graz...

  15. Barriers to Enrollment in Health Coverage in Colorado.

    Science.gov (United States)

    Martin, Laurie T; Bharmal, Nazleen; Blanchard, Janice C; Harvey, Melody; Williams, Malcolm

    2015-03-20

    As part of the implementation of the Affordable Care Act, Colorado has expanded Medicaid and also now operates its own health insurance exchange for individuals (called Connect for Health Colorado). As of early 2014, more than 300,000 Coloradans have newly enrolled in Medicaid or health insurance through Connect for Health Colorado, but there also continues to be a diverse mix of individuals in Colorado who remain eligible for but not enrolled in either private insurance or Medicaid. The Colorado Health Foundation commissioned the RAND Corporation to conduct a study to better understand why these individuals are not enrolled in health insurance coverage and to develop recommendations for how Colorado can strengthen its outreach and enrollment efforts during the next open enrollment period, which starts in November 2014. RAND conducted focus groups with uninsured and newly insured individuals across the state and interviews with local stakeholders responsible for enrollment efforts in their regions. The authors identified 11 commonly cited barriers, as well as several that were specific to certain regions or populations (such as young adults and seasonal workers). Collectively, these barriers point to a set of four priority recommendations that stakeholders in Colorado may wish to consider: (1) Support and expand localized outreach and tailored messaging; (2) Strengthen marketing and messaging to be clear, focused on health benefits of insurance (rather than politics and mandates), and actionable; (3) Improve the clarity and transparency of insurance and health care costs and enrollment procedures; and (4) Revisit the two-stage enrollment process and improve Connect for Health Colorado website navigation and technical support.

  16. The Colorado Plateau II : Biophysical, Socioeconomic, and Cultural Research

    OpenAIRE

    Van Riper, Charles; Mattson, David J.

    2005-01-01

    Abstract from GoogleBooks: The publication of The Colorado Plateau: Cultural, Biological, and Physical Research in 2004 marked a timely summation of current research in the Four Corners states. This new volume, derived from the seventh Biennial Conference on the Colorado Plateau in 2003, complements the previous book by again focusing on the integration of science into resource management issues. The 32 chapters range in content from measuring human impacts on cultural resources, through graz...

  17. Histological characterization of gell formation and lesion development on leaves of Phaseolus vulgaris and clones of hybrid poplar after exposure to simulated sulfate acid rain

    Energy Technology Data Exchange (ETDEWEB)

    Dacosta, F.

    1978-01-01

    Histological investigations with leaves of several hybrid poplar clones illustrate gall formations in response to simulated acid rain that result from hyperplasia and hypertrophy of mesophyll cells. Similar experiments with phaseolus vulgaris and clones of hybrid poplar show a sequence of events that follow a general pattern of adaxial epidermis destruction, injury to palisade parenchyma and eventual destruction of more interior tissues after continued exposure to one, six-minute, rain event daily. Results show that most (95%) lesions on Phaseolus vulgaris developed near trichomes and stomata after exposure to the simulated acid rain.

  18. Economic impact study of the Uranium Mill Tailings Remedial Action Project in Colorado: Colorado State fiscal year 1994. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    1994-12-01

    The Colorado economic impact study summarizes employment and economic benefits to the state from activities associated with the Uranium Mill Tailings Remedial Action (UMTRA) Project during Colorado state fiscal year 1994 (1 July 1993 through 30 June 1994). To capture employment information, a questionnaire was distributed to subcontractor employees at the active UMTRA Project sites of Grand Junction, Naturita, Gunnison, and Rifle, Colorado. Economic data were requested from each site prime subcontractor, as well as from the Remedial Action Contractor. Information on wages, taxes, and subcontract expenditures in combination with estimates and economic multipliers is used to estimate the dollar economic benefits to Colorado during the state fiscal year. Finally, the fiscal year 1994 estimates are compared to fiscal year 1993 employment and economic information.

  19. Mise au point d'une technique de culture in vitro d'embryons immatures de Phaseolus

    Directory of Open Access Journals (Sweden)

    Véronique Schmit

    1997-01-01

    Full Text Available Development of an in vitro culture technique for immature Phaseolus embryos. In the interspecific crosses Phaseolus polyanthus (or P. coccineus (i? x P. vulgaris, the hybrid embryos abort very early. Therefore, it is essentiel to develop an in vitro culture technique that allows the rescue of beau embryos at globular or early heart-shaped stages. After several trials conceming the salts composition, the sugar rate and the amino acid concentration of différent in vitro culture media, a technique has been developed for heart-shaped Phaseolus embryos. This technique consists of two stages. In a first step, embryos are cultivated under darkness until their germination on a medium containing the salts of Gamborg et al. (1968, 400 mg . 1` (5mM - 1 -' NHNO,, 1 mg . 1-' thiamine HCI, 5 mg . l` nicotinic acid, 0.5 mg - l` pyridoxine, 1,000 mg . l` -glutamine, 1,000 mg . l` casein hydrolysate, 100 mg . l` myo-inositol, 0.028 mg . P N6-benzyladenine, 30 g . l` sucrase, and 8 g -1-' DIFCO agar. After germination, the embryos are cultivated under light on a second medium that does not contain any NHNO, complément and is poorer in amino acids (100 mg • 1-' L-glutamine. Developed with six deys old heart-shaped embryos of the P. vulgaris Bico de Ouro (NI 637 variety, this technique has proved its efficiency with other P. vulgaris and P. polyanthus génotypes. It allows an average régénération rate of 30% from the total number of cultivated embryos.

  20. Diversity in the rhizobia associated with Phaseolus vulgaris L. in Ecuador, and comparisons with Mexican bean rhizobia.

    Science.gov (United States)

    Bernal, G; Graham, P H

    2001-06-01

    Common beans (Phaseolus vulgaris L.) have centers of origin in both Mesoamerica and Andean South America, and have been domesticated in each region for perhaps 5000 years. A third major gene pool may exist in Ecuador and Northern Peru. The diversity of the rhizobia associated with beans has also been studied, but to date with an emphasis on the Mesoamerican center of origin. In this study we compared bean rhizobia from Mexico and Andean South America using both phenotypic and phylogenetic approaches. When differences between the rhizobia of these two regions were shown, we then examined the influence of bean cultivar on the most probable number (MPN) count and biodiversity of rhizobia recovered from different soils. Three clusters of bean rhizobia were distinguished using phenotypic analysis and principal-component analysis of Box AIR-PCR banding patterns. They corresponded principally to isolates from Mexico, and the northern and southern Andean regions, with isolates from southern Ecuador exhibiting significant genetic diversity. Rhizobia from Dalea spp., which are infective and effective on beans, may have contributed to the apparent diversity of rhizobia recovered from the Mesoamerican region, while the rhizobia of wild Phaseolus aborigineus from Argentina showed only limited similarity to the other bean rhizobia tested. Use of P. vulgaris cultivars from the Mesoamerican and Andean Phaseolus gene pools as trap hosts did not significantly affect MPN counts of bean rhizobia from the soils of each region, but did influence the diversity of the rhizobia recovered. Such differences in compatibility of host and Rhizobium could be a factor in the poor reputation for nodulation and N2 fixation in this crop.

  1. Effect of volatile substances released from Origanum majorana and Ocimum basilicum on the rhizosphere and phyllosphere fungi of Phaseolus vulgaris.

    Science.gov (United States)

    Afifi, A F

    1978-01-01

    Differences were found in the counts and occurrence of fungi in the phyllosphere and thizosphere of two representatives of the Lamiacea family, Origanum majorana and Ocimum basilicum, and in the phyllosphere and rhizosphere of Phaseolus vulgaris growing separately or in coenosis with O. majorana or O. basilicum. Both the volatile substances released from ground leaves of the two latter plant species and the root exudates affected considerably spore germination of isolated phylospheric and rhizospheric fungi. The results indicated a possible role of root exudates and volatile substances released from leaves in colonization of rhizosphere and/or phyllosphere by fungi, especially in associations of various plants.

  2. Comparisons of phaseolin type andα-amylase inhibitor in common bean (Phaseolus vulgaris L.) in China

    Institute of Scientific and Technical Information of China (English)

    Yang Yao; Yibo Hu; Yingying; Zhu Yue Gao; Guixing Ren

    2016-01-01

    The objective of this study was to characterize the phaseolin type andα-amylase (αAI) level in common bean (Phaseolus vulgaris L.) accessions deposited in the Chinese National Genebank. The 40 accessions sampled were common varieties originating in Asia, North America, South America, Europe, and Africa. No Inca (I-) phaseolin was observed in the accessions. Only four accessions contained Tendergreen (T-) phaseolin and the remaining 36 contained Sanilac (S-) phaseolin. αAI proteins extracted from nine accessions showed higher α-amylase inhibitory activity than the control (Phase 2, IC50=0.65μg). These common bean accessions have potential use as nutraceutical ingredients.

  3. The seed coat of Phaseolus vulgaris interferes with the development of the cowpea weevil [Callosobruchus maculatus (F. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Luciana B. Silva

    2004-03-01

    Full Text Available We have confirmed here that the seeds of the common bean (Phaseolus vulgaris, L. do not support development of the bruchid Callosobruchus maculatus (F., a pest of cowpea [Vigna unguiculata (L. Walp] seeds. Analysis of the testa (seed coat of the bean suggested that neither thickness nor the levels of compounds such as tannic acid, tannins, or HCN are important for the resistance. On the other hand, we have found that phaseolin (vicilin-like 7S storage globulin, detected in the testa by Western blotting and N-terminal amino acid sequencing, is detrimental to the development of C. maculatus. As for the case of other previously studied legume seeds (Canavalia ensiformis and Phaseolus lunatus we suggest that the presence of vicilin-like proteins in the testa of P. vulgaris may have had a significant role in the evolutionary adaptation of bruchids to the seeds of leguminous plants.Neste trabalho confirmamos a resistência do feijão comum ( Phaseolus vulgaris ao bruquídeo Callosobruchus maculatus (F., inseto que preda sementes de feijão-de-corda (Vigna unguiculata (L.. A resistência desta semente não está relacionada com a espessura do tegumento nem com os níveis de diversos compostos como ácido taníco, fenóis e ácido cianídrico conforme demonstram nossos resultados. No entanto, faseolina, detectada no tegumento por ‘‘Western blotting’’ e seqüenciamento N-terminal, é tóxica a C. maculatus. Esses dados estão de acordo com estudos anteriores feitos com duas outras sementes de leguminosas (Canavalia ensiformis e Phaseolus lunatus e nos levam a sugerir que a presença de proteínas do tipo vicilina no tegumento de sementes de leguminosas tiveram papel importante nos mecanismo de adaptação de bruquídeos a sementes de leguminosas.

  4. Correlation of protein content to flatulent galactooligosaccharides and exogenous amino acids in seeds of Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Ryszard Kosson

    2014-02-01

    Full Text Available In order to estimate the possible correlations among constituents of Phaseolus vulgaris seeds, the contents of protein, exogenous amino acids and flatulent galactooligosaccharides (raffinose and stachyose were analyzed in 16 Polish bean cultivars for dry seeds. A negative correlation coefficient (r =-0.9490 was found between protein and methionine contents. High positive correlations among exogenous amino acids, such as lysine and isoleucine, valine and isoleucine, lysine and tyrosine, were observed indicating a chance of selecting far more than one at a time. The small-seeded bean cultivars contained higher contents of protein and galactooligosaccharides than the large-seeded ones.

  5. Geologic map of Colorado National Monument and adjacent areas, Mesa County, Colorado

    Science.gov (United States)

    Scott, Robert B.; Harding, Anne E.; Hood, William C.; Cole, Rex D.; Livaccari, Richard F.; Johnson, James B.; Shroba, Ralph R.; Dickerson, Robert P.

    2001-01-01

    New 1:24,000-scale geologic mapping in the Colorado National Monument Quadrangle and adjacent areas, in support of the USGS Western Colorado I-70 Corridor Cooperative Geologic Mapping Project, provides new interpretations of and data for the stratigraphy, structure, geologic hazards in the area from the Colorado River in Grand Valley onto the Uncompahgre Plateau. The plateau drops abruptly along northwest-trending structures toward the northeast 800 m to the Redlands area and the Colorado River in Grand Valley. In addition to common alluvial and colluvial deposits, surficial deposits include Holocene and late Pleistocene charcoal-bearing valley-fill deposits, late to middle Pleistocene river-gravel terrace deposits, Holocene to middle Pleistocene younger, intermediate, and old fan-alluvium deposits, late to middle Pleistocene local gravel deposits, Holocene to late Pleistocene rock-fall deposits, Holocene to middle Pleistocene young and old landslide deposits, Holocene to late Pleistocene sheetwash deposits and eolian deposits, and Holocene Cienga-type deposits. Only the lowest part of the Upper Cretaceous Mancos Shale is exposed in the map area near the Colorado River. The Upper and Lower? Cretaceous Dakota Formation and the Lower Cretaceous Burro Canyon Formation form resistant dipslopes in the Grand Valley and a prominent ridge on the plateau. Less resistant strata of the Upper Jurassic Morrison Formation consisting of the Brushy Basin, Salt Wash, and Tidwell Members form slopes on the plateau and low areas below the mountain front of the plateau. The Middle Jurassic Wanakah Formation nomenclature replaces the previously used Summerville Formation. Because an upper part of the Middle Jurassic Entrada Formation is not obviously correlated with strata found elsewhere, it is therefore not formally named; however, the lower rounded cliff former Slickrock Member is clearly present. The Lower Jurassic silica-cemented Kayenta Formation forms the cap rock for the Lower

  6. Colorado Better Buildings Project Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Strife, Susie; Yancey, Lea

    2013-12-30

    The Colorado Better Buildings project intended to bring new and existing energy efficiency model programs to market with regional collaboration and funding partnerships. The goals for Boulder County and its program partners were to advance energy efficiency investments, stimulate economic growth in Colorado and advance the state’s energy independence. Collectively, three counties set out to complete 9,025 energy efficiency upgrades in 2.5 years and they succeeded in doing so. Energy efficiency upgrades have been completed in more than 11,000 homes and businesses in these communities. Boulder County and its partners received a $25 million BetterBuildings grant from the U.S. Department of Energy under the American Recovery and Reinvestment Act in the summer of 2010. This was also known as the Energy Efficiency and Conservation Block Grants program. With this funding, Boulder County, the City and County of Denver, and Garfield County set out to design programs for the residential and commercial sectors to overcome key barriers in the energy upgrade process. Since January 2011, these communities have paired homeowners and business owners with an Energy Advisor – an expert to help move from assessment to upgrade with minimal hassle. Pairing this step-by-step assistance with financing incentives has effectively addressed many key barriers, resulting in energy efficiency improvements and happy customers. An expert energy advisor guides the building owner through every step of the process, coordinating the energy assessment, interpreting results for a customized action plan, providing a list of contractors, and finding and applying for all available rebates and low-interest loans. In addition to the expert advising and financial incentives, the programs also included elements of social marketing, technical assistance, workforce development and contractor trainings, project monitoring and verification, and a cloud-based customer data system to coordinate among field

  7. Approaches to local climate action in Colorado

    Science.gov (United States)

    Huang, Y. D.

    2011-12-01

    Though climate change is a global problem, the impacts are felt on the local scale; it follows that the solutions must come at the local level. Fortunately, many cities and municipalities are implementing climate mitigation (or climate action) policies and programs. However, they face many procedural and institutional barriers to their efforts, such of lack of expertise or data, limited human and financial resources, and lack of community engagement (Krause 2011). To address the first obstacle, thirteen in-depth case studies were done of successful model practices ("best practices") of climate action programs carried out by various cities, counties, and organizations in Colorado, and one outside Colorado, and developed into "how-to guides" for other municipalities to use. Research was conducted by reading documents (e.g. annual reports, community guides, city websites), email correspondence with program managers and city officials, and via phone interviews. The information gathered was then compiled into a series of reports containing a narrative description of the initiative; an overview of the plan elements (target audience and goals); implementation strategies and any indicators of success to date (e.g. GHG emissions reductions, cost savings); and the adoption or approval process, as well as community engagement efforts and marketing or messaging strategies. The types of programs covered were energy action plans, energy efficiency programs, renewable energy programs, and transportation and land use programs. Between the thirteen case studies, there was a range of approaches to implementing local climate action programs, examined along two dimensions: focus on climate change (whether it was direct/explicit or indirect/implicit) and extent of government authority. This benchmarking exercise affirmed the conventional wisdom propounded by Pitt (2010), that peer pressure (that is, the presence of neighboring jurisdictions with climate initiatives), the level of

  8. Puente Río Colorado - Costa Rica

    Directory of Open Access Journals (Sweden)

    Kulka, F.

    1973-03-01

    Full Text Available The Colorado River bridge is located in a 95 m deep canyon, with a 122 m span. To choose the type of bridge, it has been endeavoured to use the largest possible number of national building materials which, together with the difficulty of reaching the site, meant that a series of classical solutions had to be rejected. That of an arch bridge was adopted, with a reversed support on prestressed cables, on which the road passes. The system is based on the hanging bridge principle, but with the rolling track resting on the cables, instead of hanging from them. There is a first cover, made up of prefabricated components, on the cables, which strengthens the bridge's stability. This cover supports three portal-columns, the pillars of the final roadway. The cables were prestressed from the heads of the two sloping pillars. The two side spans were designed with prefabricated T girders.El puente Río Colorado está situado en un cañón de 95 m de profundidad, salvando una luz de 122 m. Para la elección del tipo de puente se ha procurado emplear el mayor número posible de materiales de construcción nacionales, lo que, unido a la dificultad de acceso a la obra, hizo que se rechazaran una serie de soluciones clásicas. Se adoptó la de un puente-arco con un soporte invertido sobre cables pretensados, encima del cual descansa la calzada. El sistema está basado en los principios del puente colgante, pero apoyando el camino de rodadura en los cables, en lugar de colgarlo de ellos. Sobre los cables existe una primera cubierta, integrada por elementos prefabricados, que refuerza la estabilidad del puente. Esta cubierta soporta tres pórticos-columna, pilares de la calzada definitiva. El pretensado de los cables se realizó desde las cabezas de dos pilares inclinados. Los dos vanos laterales se proyectaron con vigas en T prefabricadas.

  9. Characterization of Two Putative Protein Phosphatase Genes and Their Involvement in Phosphorus Efficiency in Phaseolus vulgari

    Institute of Scientific and Technical Information of China (English)

    Cui-Yue Liang; Zhi-Jian Chen; Zhu-Fang Yao; Jiang Tian; Hong Liao

    2012-01-01

    Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses.In this study,two putative protein phosphatases,PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris).The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:GFP in onion epidermal cells.Transcripts of the two PvPS2 genes were significantly increased by phosphate (Pi) starvation in the two bean genotypes,G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype).However,G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during P1 starvation.Increased transcription of PvPS2:1 in response to Pi starvation was further verified through histochemical analysis of PvPS2:1 promoter fusion β-glucuronidase (GUS) in transgenic Arabidopsis plants.Analysis of PvPS2∶1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2:1 was involved in root growth and P accumulation.Furthermore,expression levels of two P(1) starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2∶1 Arabidopsis lines.Taken together,our results strongly suggested that PvPS2∶1positively regulated plant responses to P1 starvation,and could be further targeted as a candidate gene to improve crop P efficiency.

  10. A PHASEOLUS VULGARIS EXTRACT REDUCES CUE-INDUCED REINSTATEMENT OF CHOCOLATE SEEKING IN RATS

    Directory of Open Access Journals (Sweden)

    Irene eLorrai

    2016-04-01

    Full Text Available Previous evidence has suggested that treatment with a standardized dry extract of Phaseolus vulgaris reduced intake and operant self-administration of highly palatable foods and fluids in rats and mice. The present study was designed to assess whether such extract was also effective in reducing seeking behavior for a highly hedonic chocolate-flavored beverage, using a reinstatement procedure adopted from the drug addiction research field and modeling relapse behavior. Rats were initially trained to lever-respond for the chocolate-flavored beverage under the Fixed Ratio (FR 10 (FR10 schedule of reinforcement. Subsequently, rats were exposed to an extinction responding phase, during which lever-responding – being unreinforced – diminished progressively up to extinction. Lever-responding was then powerfully reinstated by the non-contingent presentation of a complex of gustatory, olfactory, auditory, and visual stimuli previously associated to the availability of the chocolate-flavored beverage. Acute, intragastric administration of P. vulgaris dry extract (100 and 500 mg/kg reduced lever-responding by 40-45%, in comparison to vehicle condition. These results indicate the ability of P. vulgaris dry extract to reduce seeking behavior for a highly palatable nourishment in an experimental model of relapse into disordered eating of palatable foods. The unavailability of the chocolate-flavored beverage in the reinstatement session tends to exclude that the observed effect of the P. vulgaris dry extract was secondary to any inhibition of carbohydrate metabolism; conversely, it is the likely consequence on a central action on the rewarding and hedonic properties of food.

  11. Photochemical activity of primary leaves in cadmium stressed Phaseolus coccineus depends on their growth stages

    Directory of Open Access Journals (Sweden)

    Ewa Skórzyńska-Polit

    2014-01-01

    Full Text Available Runner bean plants (Phaseolus coccineus L., cv. Piękny Jaś grown hydroponically were treated with Cd (2.5 • 10-5 M Cd in the form of 3CdSO4 • 8H2O at different stages of primary leaves development. Ten days after the metal treatment, the changes in leaf growth parameters and their PSII photochemistry were investigated. The younger were the plants exposed to Cd (at the initial growth stage of primary leaves the stronger was the leaf area reduction, fresh weight decrease and increase of plastid pigment level. These changes were correlated with lower level of photosynthetic 02 evolution (75% of control but chlorophyll a fluorescence measurements suggested an inhibitory effect of Cd on the acceptor side of PSII. With the development of primary leaves the sensitivity of plants to Cd increased. Cd treatment of plants at the final growth stage of the primary leaves caused slight changes in growth parameters compared with control, whereas the chlorophyll content and PSII activity (48% of control significantly decreased. Analyses of fast chlorophyll a fluorescence kinetics revealed changes suggesting Cd-induced alterations of PSII complex extending on to their acceptor side and the reaction centre. Differential response of primary leaves to Cd depending on their growth stages indicated alterations of the adaptation mechanism in leaf cells of runner bean plants. The decrease in younger Cd-treated plants of photosynthetic activity was connected with disturbances in growth processes, however in plants treated with Cd at the final growth stage of the primary leaves it was resulted from destruction of the photosynthetic apparatus.

  12. A Phaseolus vulgaris Extract Reduces Cue-Induced Reinstatement of Chocolate Seeking in Rats

    Science.gov (United States)

    Lorrai, Irene; Piga, Valentina; Carai, Mauro A. M.; Riva, Antonella; Morazzoni, Paolo; Gessa, Gian Luigi; Colombo, Giancarlo; Maccioni, Paola

    2016-01-01

    Previous evidence has suggested that treatment with a standardized dry extract of Phaseolus vulgaris reduced intake and operant self-administration of highly palatable foods and fluids in rats and mice. The present study was designed to assess whether such extract was also effective in reducing seeking behavior for a highly hedonic chocolate-flavored beverage, using a “reinstatement” procedure adopted from the drug addiction research field and modeling relapse behavior. Rats were initially trained to lever-respond for the chocolate-flavored beverage under the Fixed Ratio (FR) 10 schedule of reinforcement. Subsequently, rats were exposed to an extinction responding phase, during which lever-responding – being unreinforced – diminished progressively up to extinction. Lever-responding was then powerfully reinstated by the non-contingent presentation of a complex of gustatory, olfactory, auditory, and visual stimuli previously associated to the availability of the chocolate-flavored beverage. Acute, intragastric administration of P. vulgaris dry extract (100 and 500 mg/kg) reduced lever-responding by 40–45%, in comparison to vehicle condition. These results indicate the ability of P. vulgaris dry extract to reduce seeking behavior for a highly palatable nourishment in an experimental model of relapse into disordered eating of palatable foods. The unavailability of the chocolate-flavored beverage in the reinstatement session tends to exclude that the observed effect of the P. vulgaris dry extract was secondary to any inhibition of carbohydrate metabolism; conversely, it is the likely consequence on a central action on the rewarding and hedonic properties of food. PMID:27199752

  13. Annotation and sequence diversity of transposable elements in common bean (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Scott eJackson

    2014-07-01

    Full Text Available Common bean (Phaseolus vulgaris is an important legume crop grown and consumed worldwide. With the availability of the common bean genome sequence, the next challenge is to annotate the genome and characterize functional DNA elements. Transposable elements (TEs are the most abundant component of plant genomes and can dramatically affect genome evolution and genetic variation. Thus, it is pivotal to identify TEs in the common bean genome. In this study, we performed a genome-wide transposon annotation in common bean using a combination of homology and sequence structure-based methods. We developed a 2.12-Mb transposon database which includes 791 representative transposon sequences and is available upon request or from www.phytozome.org. Of note, nearly all transposons in the database are previously unrecognized TEs. More than 5,000 transposon-related expressed sequence tags (ESTs were detected which indicates that some transposons may be transcriptionally active. Two Ty1-copia retrotransposon families were found to encode the envelope-like protein which has rarely been identified in plant genomes. Also, we identified an extra open reading frame (ORF termed ORF2 from 15 Ty3-gypsy families that was located between the ORF encoding the retrotransposase and the 3’LTR. The ORF2 was in opposite transcriptional orientation to retrotransposase. Sequence homology searches and phylogenetic analysis suggested that the ORF2 may have an ancient origin, but its function is not clear. This transposon data provides a useful resource for understanding the genome organization and evolution and may be used to identify active TEs for developing transposon-tagging system in common bean and other related genomes.

  14. Impact of management strategies in the basal rot, charcoal rots epidemiology and Phaseolus vulgaris L. yield.

    Directory of Open Access Journals (Sweden)

    Ulacio Osorio Dilcia

    2013-02-01

    Full Text Available The effect of chemical, physical, biologycal and cultural strategies individually or combinated were evaluated in the epidemiology of the basal rot (Sclerotium rolfsii, charcoal rot (Macrophomina phaseolina and the Phaseolus vulgaris cv Tacarigua yield at Barinas state from Venezuela. In the experiment, Tebuconazole (Teb was applicated at seed (1 L/Ton and at soil, a los 30 y 60 days after of the sow (1 L/ha; Trichoderma harzianum (Tri was applicated at seed (15 g for each 1.5 k and to 15, 30, 45 y 60 days after of the sow (30 g/10 L of water. On the other hand, soil was solarizated (Sol during 15 days and calcium nitrate (Ca (60 g/10 L of water was applicated each 15 days until 60 days of growth of cultivated plants. Basal rot was registered as far as 42 days after of the sow, showing less of 5.3% in Teb y the combination SolTeb. The hightest incidence of this disease was observed in the treatment Tri with 28.5%, being highter that control (14.5%. Last to 42 days predominated the charcoal rot in the rest of the plants for a total of 100% of incidente in everything the treatments. Nevertheless, Teb showed the hightest yield with 555 k/ha, being different estatistically at treatment TriCa, which showed the lowest yield with 31 k/ha, however, the roots not formed nodules nitrogen uptake in these replications with the fungicide and Ca. It is concluded that S. rolfsii was sensible at action of some of the treatments; but not M. phaseolina; nevertheless, the plants were capables to produce seeds health apparently in treatments in which observed less severity of charcoal rot.

  15. Constraints to hydraulic acclimation under reduced light in two contrasting Phaseolus vulgaris cultivars.

    Science.gov (United States)

    Matzner, Steven L; Rettedal, David D; Harmon, Derek A; Beukelman, MacKenzie R

    2014-08-01

    Two cultivars of Phaseolus vulgaris L. were grown under three light levels to determine if hydraulic acclimation to light occurs in herbaceous annuals and whether intraspecific trade-offs constrain hydraulic traits. Acclimation occurred in response to reduced light and included decreased stomatal density (SD) and increased specific leaf area (SLA). Reduced light resulted in lower wood density (WD); decreased cavitation resistance, measured as the xylem pressure causing a 50 % reduction in stem conductivity (P50); and increased hydraulic capacity, measured as average leaf mass specific transpiration (E(LM)). Significant or marginally significant trade-offs between P50 and WD, WD and E(LM), and E(LM) and P50 reflected variation due to both genotype and environmental effects. A trade-off between WD and P50 within one cultivar indicated that morphological adjustment was constrained. Coordinated changes in WD, P50, and E(LM) within each cultivar in response to light were consistent with trade-offs constraining plasticity. A water-use efficiency (WUE, measured as δ(13)C) versus hydraulic capacity (E(LM)) trade-off was observed within each cultivar, further indicating that hydraulic trade-offs can constrain acclimation. Larger plants had lower hydraulic capacity (E(LM)) but greater cavitation resistance, WD, and WUE. Distinct hydraulic strategies were observed with the cultivar adapted to irrigated conditions having higher stomatal conductance and stem flow rates. The cultivar adapted to rain-fed conditions had higher leaf area and greater cavitation resistance. Hydraulic trade-offs were observed within the herbaceous P. vulgaris resulting from both genotype and environmental effects. Trade-offs within a cultivar reflected constraints to hydraulic acclimation in response to changing light.

  16. Pengaruh Varietas dan Waktu Penyiangan Gulma Terhadap Pertumbuhan dan Hasil Tanaman Kacang Hijau (Phaseolus Radiatus L.

    Directory of Open Access Journals (Sweden)

    EUSEBIO GOMES

    2015-06-01

    Full Text Available The Effects of Variety and Weeding Times on Growth and Yield of Mungbean (PhaseolusRadiatus L.The mungbean have a great potential as a food ingredient mixture of refined productsand has certain competitive advantages over other types of beans. Mungbean production in East Timoris still low and has not been able to meet the needs of the market. Therefore, weed control and selectionof good varieties of mung beans is one of the determining factors in the increased production of mungbean.The objective of the study was to determine the effect of time of weeding on the growth and yield ofseveral varieties of mungbean. The study was conducted at the Pilot Suco Hera Sub-distrito Cristo Rei,Dili-Timor Leste. It used a randomized block design with two factors. The first factor was type ofvarieties of mungbean and the second factor was the weeding time. Factor of types of varieties consistedof three, namely: VL = Local Variety, VM = Merak variety, VG = Gelatik variety, while factor ofweeding times consists of PO = No weeding, P1 = weeding at 2 and 4 weeks after planting, P2 =weeding at 3 and 5 weeks after planting, with three replications. The results showed that weeding timeaffected the outcome of drying seed dry weight per hectare. Weeding time at 2 and 4 weeks afterplanting had a better dry weight of seed i.e 1.09 g, compared to those of weeding at 3 and 5 weeksafter planting. The use of different varieties showed that Merak variety had drying seed dry weight of1.16 tons ha-1, Gelatik variety was 1.09 tons ha-1, and the local variety was 0.97 tons ha-1.

  17. A gap analysis methodology for collecting crop genepools: a case study with phaseolus beans.

    Directory of Open Access Journals (Sweden)

    Julián Ramírez-Villegas

    Full Text Available BACKGROUND: The wild relatives of crops represent a major source of valuable traits for crop improvement. These resources are threatened by habitat destruction, land use changes, and other factors, requiring their urgent collection and long-term availability for research and breeding from ex situ collections. We propose a method to identify gaps in ex situ collections (i.e. gap analysis of crop wild relatives as a means to guide efficient and effective collecting activities. METHODOLOGY/PRINCIPAL FINDINGS: The methodology prioritizes among taxa based on a combination of sampling, geographic, and environmental gaps. We apply the gap analysis methodology to wild taxa of the Phaseolus genepool. Of 85 taxa, 48 (56.5% are assigned high priority for collecting due to lack of, or under-representation, in genebanks, 17 taxa are given medium priority for collecting, 15 low priority, and 5 species are assessed as adequately represented in ex situ collections. Gap "hotspots", representing priority target areas for collecting, are concentrated in central Mexico, although the narrow endemic nature of a suite of priority species adds a number of specific additional regions to spatial collecting priorities. CONCLUSIONS/SIGNIFICANCE: Results of the gap analysis method mostly align very well with expert opinion of gaps in ex situ collections, with only a few exceptions. A more detailed prioritization of taxa and geographic areas for collection can be achieved by including in the analysis predictive threat factors, such as climate change or habitat destruction, or by adding additional prioritization filters, such as the degree of relatedness to cultivated species (i.e. ease of use in crop breeding. Furthermore, results for multiple crop genepools may be overlaid, which would allow a global analysis of gaps in ex situ collections of the world's plant genetic resources.

  18. Comparative analysis of genome-wide Mlo gene family in Cajanus cajan and Phaseolus vulgaris.

    Science.gov (United States)

    Deshmukh, Reena; Singh, V K; Singh, B D

    2016-04-01

    The Mlo gene was discovered in barley because the mutant 'mlo' allele conferred broad-spectrum, non-race-specific resistance to powdery mildew caused by Blumeria graminis f. sp. hordei. The Mlo genes also play important roles in growth and development of plants, and in responses to biotic and abiotic stresses. The Mlo gene family has been characterized in several crop species, but only a single legume species, soybean (Glycine max L.), has been investigated so far. The present report describes in silico identification of 18 CcMlo and 20 PvMlo genes in the important legume crops Cajanus cajan (L.) Millsp. and Phaseolus vulgaris L., respectively. In silico analysis of gene organization, protein properties and conserved domains revealed that the C. cajan and P. vulgaris Mlo gene paralogs are more divergent from each other than from their orthologous pairs. The comparative phylogenetic analysis classified CcMlo and PvMlo genes into three major clades. A comparative analysis of CcMlo and PvMlo proteins with the G. max Mlo proteins indicated close association of one CcMlo, one PvMlo with two GmMlo genes, indicating that there was no further expansion of the Mlo gene family after the separation of these species. Thus, most of the diploid species of eudicots might be expected to contain 15-20 Mlo genes. The genes CcMlo12 and 14, and PvMlo11 and 12 are predicted to participate in powdery mildew resistance. If this prediction were verified, these genes could be targeted by TILLING or CRISPR to isolate powdery mildew resistant mutants.

  19. Physical, biochemical and physiological effects of ultraviolet radiation on Brassica napus and Phaseolus vulgaris

    Energy Technology Data Exchange (ETDEWEB)

    Cen Yan-Ping

    1993-12-31

    In order to follow some of the changes induced by ultraviolet-B (UV-B, 280-320 nm) radiation in Phaseolus vulgaris and Brassica napus, experiments were designed to localize sites of changes in leaves and to correlate some of the physiological and biochemical changes with penetration of UV-B radiation. B.napus was exposed to 8.9 kJ m{sup -2} day{sup -1} biologically effective UV-B radiation (UV-B{sub BE}). The penetration of UV-B radiation into the leaf was followed using a quartz fibre optic microprobe. Monochromatic radiation at 310 nm was decreased by ca 50 and 34% in the adaxial and abaxial epidermis, respectively, in plants not exposed to UV-B, whereas the radiation was decreased by ca 70 and 42%, respectively, in the same region in UV-treated plants. Polychromatic radiation showed a wavelength dependent change mainly for the collimated radiation. The results correlated with the distribution of phenolic compounds analysed from 40 {mu}m paradermal leaf sections. The first adaxial section (40{mu}m) contained 35% of the whole leaf sample flavonoid glycosides in control plants, and 66% in UV-treated plants. Hydroxycinnamic acid derivatives increased by 26% in UV-treated plants relative to controls. The ratio of quercetin to kaempferol derivatives increased from 0.11 in controls to 0.91 in leaves of UV-treated plants. The leaf epidermis protected the inner leaf tissue where most of the photosynthetic apparatus is located. P. vulgaris was subjected to 6.17 kJ m{sup -2} day{sup -1} UV-B{sub BE} with different levels of visible light. The largest UV-induced changes in photosynthesis, chlorophyll, carotenoids, UV-screening pigments, and surface leaf reflectance occurred under growth conditions of low levels of visible light together with UV radiation.

  20. Rhizobium hidalgonense sp. nov., a nodule endophytic bacterium of Phaseolus vulgaris in acid soil.

    Science.gov (United States)

    Yan, Jun; Yan, Hui; Liu, Li Xue; Chen, Wen Feng; Zhang, Xiao Xia; Verástegui-Valdés, Myrthala M; Wang, En Tao; Han, Xiao Zeng

    2017-01-01

    One Gram-negative, aerobic, motile, rod-shaped bacterium, designated as FH14(T), was isolated from nodules of Phaseolus vulgaris grown in Hidalgo State of Mexico. Results based upon 16S rRNA gene (≥99.8 % similarities to known species), concatenated sequence (recA, atpD and glnII) analysis of three housekeeping genes (≤93.4 % similarities to known species) and average nucleotide identity (ANI) values of genome sequence (ranged from 87.6 to 90.0 % to related species) indicated the distinct position of strain FH14(T) within the genus Rhizobium. In analyses of symbiotic genes, only nitrogen fixation gene nifH was amplified that had nucleotide sequence identical to those of the bean-nodulating strains in R. phaseoli and R. vallis, while nodulation gene nodC gene was not amplified. The failure of nodulation to its original host P. vulgaris and other legumes evidenced the loss of its nodulation capability. Strain FH14(T) contained summed feature 8 (C18:1 ω6c/C18:1 ω7c, 59.96 %), C16:0 (10.6 %) and summed feature 2 (C12:0 aldehyde/unknown 10.928, 10.24 %) as the major components of cellular fatty acids. Failure to utilize alaninamide, and utilizing L-alanine, L-asparagine and γ-amino butyric acid as carbon source, distinguished the strain FH14(T) from the type strains for the related species. The genome size and DNA G+C content of FH14(T) were 6.94 Mbp and 60.8 mol %, respectively. Based on those results, a novel specie in Rhizobium, named Rhizobium hidalgonense sp. nov., was proposed, with FH14(T) (=HAMBI 3636(T) = LMG 29288(T)) as the type strain.

  1. Phaseolus vulgaris L. Seedlings Exposed to Prometryn Herbicide Contaminated Soil Trigger an Oxidative Stress Response.

    Science.gov (United States)

    Boulahia, Kerima; Carol, Pierre; Planchais, Séverine; Abrous-Belbachir, Ouzna

    2016-04-27

    Herbicides from the family of S-triazines, such as prometryn, have been widely used in crop production and can constitute an environmental pollution in both water and soil. As a valuable crop, the common bean (Phaseolus vulgaris L.) is grown all over the world and could be exposed to such herbicides. We wanted to investigate the possible stress sustained by the common bean growing in prometryn-polluted soil. Two situations were observed: when soil was treated with ≥100 μM prometryn, some, but not all, measured growth parameters were affected in a dose-dependent manner. Growth was reduced, and photosynthetic pigments and photosynthetic products were less accumulated when soil was treated with ≥100 μM prometryn. Reactive oxygen species (ROS) produced had a deleterious effect, as seen by the accumulation of oxidized lipid in the form of malondialdehyde (MDA). Higher prometryn (500 μM) concentrations had a disastrous effect, reducing antioxidant activities. At a low (10 μM) concentration, prometryn increased antioxidant enzymatic activities without affecting plant growth or MDA production. Gene expression of proline metabolism genes and proline accumulation confirm that bean plants respond to a stress according to the prometryn concentration. Physiological responses such as antioxidative enzymes APX, CAT, and the enzyme implicated in the metabolization of xenobiotics, GST, were increased at 10 and 100 μM, which indicated a prevention of deleterious effects of prometryn, suggesting that bean is a suitable material both for herbicide pollution sensing and as a crop on a low level of herbicide pollution.

  2. Identification of novel drought-tolerant-associated SNPs in common bean (Phaseolus vulgaris).

    Science.gov (United States)

    Villordo-Pineda, Emiliano; González-Chavira, Mario M; Giraldo-Carbajo, Patricia; Acosta-Gallegos, Jorge A; Caballero-Pérez, Juan

    2015-01-01

    Common bean (Phaseolus vulgaris L.) is a leguminous in high demand for human nutrition and a very important agricultural product. Production of common bean is constrained by environmental stresses such as drought. Although conventional plant selection has been used to increase production yield and stress tolerance, drought tolerance selection based on phenotype is complicated by associated physiological, anatomical, cellular, biochemical, and molecular changes. These changes are modulated by differential gene expression. A common method to identify genes associated with phenotypes of interest is the characterization of Single Nucleotide Polymorphims (SNPs) to link them to specific functions. In this work, we selected two drought-tolerant parental lines from Mesoamerica, Pinto Villa, and Pinto Saltillo. The parental lines were used to generate a population of 282 families (F3:5) and characterized by 169 SNPs. We associated the segregation of the molecular markers in our population with phenotypes including flowering time, physiological maturity, reproductive period, plant, seed and total biomass, reuse index, seed yield, weight of 100 seeds, and harvest index in three cultivation cycles. We observed 83 SNPs with significant association (p < 0.0003 after Bonferroni correction) with our quantified phenotypes. Phenotypes most associated were days to flowering and seed biomass with 58 and 44 associated SNPs, respectively. Thirty-seven out of the 83 SNPs were annotated to a gene with a potential function related to drought tolerance or relevant molecular/biochemical functions. Some SNPs such as SNP28 and SNP128 are related to starch biosynthesis, a common osmotic protector; and SNP18 is related to proline biosynthesis, another well-known osmotic protector.

  3. The Effect of vermicompost on salt tolerance of bean seedlings (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    A. Beyk Khurmizi

    2016-04-01

    Full Text Available In the recent years, increasing production of waste as a result of population growth, increased food consumption, industrial development and urbanization growth, is regarded as a serious challenge. Vermicompost, as an end product of urban waste recycling with proper physicochemical features, can play an effective role in plant growth and development and also in reducing harmful effects of various environmental stresses on plants. For this purpose, a study with the aim of investigating the effects of vermicompost and salinity interactions on morphological traits of bean (Phaseolus vulgaris L. cv. Light Red Kidney seedlings was performed. The experiment was conducted based on a randomized complete block design, including five different volumetric ratios of vermicompost and sand (0:100; 10:90; 25:75; 50:50 and 75:25, and four levels of salinity (30, 60, 90 and 120 mmol l-1 NaCl, equal to 2.75, 5.50, 8.25 and 11 deciSiemens per meter (dS/m respectively, along with control (0.00, in three replications. Seeds were cultured in plastic pots and sampling of seedlings was done after 28 days. The results showed that in an environment without stress, vermicompost had significant effect (p ≤ 0.05 on the stem length, internodes number, area and dry weight of leaves, diameter, dry weight and total roots length, while having no significant effect on stem dry weight. The interaction between salinity and vermicompost has significant effect on the stem length, internodes number, the area and dry weight of leaves and dry weight of roots but no significant effect was observed on the stem dry weight, diameter and total roots length. Thus, in the low levels of salinity, all ratios of vermicompost and in high levels of salinity, high ratios of vermicompost can limit the negative effects of salinity on bean seedlings.

  4. Influence of atmospheric vapour pressure deficit on ozone responses of snap bean (Phaseolus vulgaris L.) genotypes.

    Science.gov (United States)

    Fiscus, Edwin L; Booker, Fitzgerald L; Sadok, Walid; Burkey, Kent O

    2012-04-01

    Environmental conditions influence plant responses to ozone (O(3)), but few studies have evaluated individual factors directly. In this study, the effect of O(3) at high and low atmospheric vapour pressure deficit (VPD) was evaluated in two genotypes of snap bean (Phaseolus vulgaris L.) (R123 and S156) used as O(3) bioindicator plants. Plants were grown in outdoor controlled-environment chambers in charcoal-filtered air containing 0 or 60 nl l(-1) O(3) (12 h average) at two VPDs (1.26 and 1.96 kPa) and sampled for biomass, leaf area, daily water loss, and seed yield. VPD clearly influenced O(3) effects. At low VPD, O(3) reduced biomass, leaf area, and seed yield substantially in both genotypes, while at high VPD, O(3) had no significant effect on these components. In clean air, high VPD reduced biomass and yield by similar fractions in both genotypes compared with low VPD. Data suggest that a stomatal response to VPD per se may be lacking in both genotypes and it is hypothesized that the high VPD resulted in unsustainable transpiration and water deficits that resulted in reduced growth and yield. High VPD- and water-stress-induced stomatal responses may have reduced the O(3) flux into the leaves, which contributed to a higher yield compared to the low VPD treatment in both genotypes. At low VPD, transpiration increased in the O(3) treatment relative to the clean air treatment, suggesting that whole-plant conductance was increased by O(3) exposure. Ozone-related biomass reductions at low VPD were proportionally higher in S156 than in R123, indicating that differential O(3) sensitivity of these bioindicator plants remained evident when environmental conditions were conducive for O(3) effects. Assessments of potential O(3) impacts on vegetation should incorporate interacting factors such as VPD.

  5. Punctuated distribution of recombination hotspots and demarcation of pericentromeric regions in Phaseolus vulgaris L.

    Science.gov (United States)

    Bhakta, Mehul S; Jones, Valerie A; Vallejos, C Eduardo

    2015-01-01

    High density genetic maps are a reliable tool for genetic dissection of complex plant traits. Mapping resolution is often hampered by the variable crossover and non-crossover events occurring across the genome, with pericentromeric regions (pCENR) showing highly suppressed recombination rates. The efficiency of linkage mapping can further be improved by characterizing and understanding the distribution of recombinational activity along individual chromosomes. In order to evaluate the genome wide recombination rate in common beans (Phaseolus vulgaris L.) we developed a SNP-based linkage map using the genotype-by-sequencing approach with a 188 recombinant inbred line family generated from an inter gene pool cross (Andean x Mesoamerican). We identified 1,112 SNPs that were subsequently used to construct a robust linkage map with 11 groups, comprising 513 recombinationally unique marker loci spanning 943 cM (LOD 3.0). Comparative analysis showed that the linkage map spanned >95% of the physical map, indicating that the map is almost saturated. Evaluation of genome-wide recombination rate indicated that at least 45% of the genome is highly recombinationally suppressed, and allowed us to estimate locations of pCENRs. We observed an average recombination rate of 0.25 cM/Mb in pCENRs as compared to the rest of genome that showed 3.72 cM/Mb. However, several hot spots of recombination were also detected with recombination rates reaching as high as 34 cM/Mb. Hotspots were mostly found towards the end of chromosomes, which also happened to be gene-rich regions. Analyzing relationships between linkage and physical map indicated a punctuated distribution of recombinational hot spots across the genome.

  6. Transcriptome analysis of salt tolerant common bean (Phaseolus vulgaris L.) under saline conditions.

    Science.gov (United States)

    Hiz, Mahmut Can; Canher, Balkan; Niron, Harun; Turet, Muge

    2014-01-01

    Salinity is one of the important abiotic stress factors that limit crop production. Common bean, Phaseolus vulgaris L., a major protein source in developing countries, is highly affected by soil salinity and the information on genes that play a role in salt tolerance is scarce. We aimed to identify differentially expressed genes (DEGs) and related pathways by comprehensive analysis of transcriptomes of both root and leaf tissues of the tolerant genotype grown under saline and control conditions in hydroponic system. We have generated a total of 158 million high-quality reads which were assembled into 83,774 all-unigenes with a mean length of 813 bp and N50 of 1,449 bp. Among the all-unigenes, 58,171 were assigned with Nr annotations after homology analyses. It was revealed that 6,422 and 4,555 all-unigenes were differentially expressed upon salt stress in leaf and root tissues respectively. Validation of the RNA-seq quantifications (RPKM values) was performed by qRT-PCR (Quantitative Reverse Transcription PCR) analyses. Enrichment analyses of DEGs based on GO and KEGG databases have shown that both leaf and root tissues regulate energy metabolism, transmembrane transport activity, and secondary metabolites to cope with salinity. A total of 2,678 putative common bean transcription factors were identified and classified under 59 transcription factor families; among them 441 were salt responsive. The data generated in this study will help in understanding the fundamentals of salt tolerance in common bean and will provide resources for functional genomic studies.

  7. Molecular cloning and characterization of the ABA-specific glucosyltransferase gene from bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Palaniyandi, Sasikumar Arunachalam; Chung, Gyuhwa; Kim, Sang Hyon; Yang, Seung Hwan

    2015-04-15

    Levels of the plant hormone abscisic acid (ABA) are maintained in homeostasis by a balance of its biosynthesis, catabolism and conjugation. The detailed molecular and signaling events leading to strict homeostasis are not completely understood in crop plants. In this study, we obtained cDNA of an ABA-inducible, ABA-specific UDP-glucosyltransferase (ABAGT) from the bean plant (Phaseolus vulgaris L.) involved in conjugation of a glucose residue to ABA to form inactive ABA-glucose ester (ABA-GE) to examine its role during development and abiotic stress in bean. The bacterially expressed PvABAGTase enzyme showed ABA-specific glucosylation activity in vitro. A higher level of the PvABAGT transcript was observed in mature leaves, mature flowers, roots, seed coats and embryos as well as upon rehydration following a period of dehydration. Overexpression of 35S::PvABAGT in Arabidopsis showed reduced sensitivity to ABA compared with WT. The transgenic plants showed a high level of ABA-GE without significant decrease in the level of ABA compared with the wild type (WT) during dehydration stress. Upon rehydration, the levels of ABA and phaseic acid (PA) decreased in the WT and the PvABAGT-overexpressing lines with high levels of ABA-GE only in the transgenic plants. Our findings suggest that the PvABAGT gene could play a role in ABA homeostasis during development and stress responses in bean and its overexpression in Arabidopsis did not alter ABA homeostasis during dehydration stress. Copyright © 2015 Elsevier GmbH. All rights reserved.

  8. Response of Phaseolus vulgaris L. plants to low-let ionizing radiation: Growth and oxidative stress

    Science.gov (United States)

    Arena, C.; De Micco, V.; Aronne, G.; Pugliese, M.; Virzo De Santo, A.; De Maio, A.

    2013-10-01

    The scenarios for the long-term habitation of space platforms and planetary stations involve plants as fundamental part of Bioregenerative Life Support Systems (BLSS) to support the crew needs. Several constraints may limit plant growth in space: among them ionizing radiation is recognized to severely affect plant cell at morphological, physiological and biochemical level. In this work, plants of Phaseolus vulgaris L. were subjected to four different doses of X-rays (0.3, 10, 50 and 100 Gy) in order to assess the effects of ionizing radiation on this species and to analyze possible mechanisms carried out to overcome the radiation injuries. The effects of X-rays on plant growth were assessed by measuring stem elongation, number of internodes and leaf dry weight. The integrity of photosynthetic apparatus was evaluated by photosynthetic pigment composition and ribulose 1,5-bisphosphate carboxylase (Rubisco) activity, whereas changes in total antioxidant pool and glutathione S transferase activity (GST) were utilized as markers of oxidative stress. The distribution of phenolic compounds in leaf tissues as natural shielding against radiation was also determined. Irradiation of plants at 0.3 and 10 Gy did not determine differences in all considered parameters as compared to control. On the contrary, at 50 and 100 Gy a reduction of plant growth and a decrease in photosynthetic pigment content, as well as an increase in phenolic compounds and a decrease in total antioxidant content and GST activity were found. Only a slight reduction of Rubisco activity in leaves irradiated at 50 and 100 Gy was found. The overall results indicate P. vulgaris as a species with a good potential to face ionizing radiation and suggest its suitability for utilization in BLSSs.

  9. Sunfleck dynamics and canopy structure in a Phaseolus vulgaris L. canopy

    Science.gov (United States)

    Barradas, Victor L.; Jones, Hamlyn G.; Clark, Jerry A.

    Photosynthetic photon flux density (PPFD) fluctuations were quantified in crops of beans (Phaseolus vulgaris L.) in the field as the canopy developed between July and October. Two different methods were used to select sunflecks and shadeflecks. Four ranges of zenith angles (60-70°, 50-60°, 40-50° and 30-40°) were selected for analysing PPFD fluctuations. At the base of the canopy, sunflecks contributed 18%, 53%, 10% and 4% during the 1st, 3rd, 5th and 7th week of growth, respectively. At a height of 20 cm above the soil surface, the respective contributions were 28% and 21% during the 6th and 7th weeks. Sunfleck lengths of 0-5 s were the most frequent, with the greatest number being found with smaller zenith angles. The proportion of short duration sunflecks increased as the growth period advanced. The number of long sunflecks decreased with time, with very few longer than 100 s by the 5th and 7th weeks. The distributions of sunfleck irradiance were similar to normal distributions and irradiance ranged in μmol m-2 s-1 from 600-900, 800-1500 and 1000-1600 respectively at zenith angles of 50-60°, 40-50° and 30-40°. A multiple regression showed that short sunflecks (100 s) depended on zenith angle and Ls. Shadefleck distributions were similar to those for sunflecks but there were fewer of the shortest examples and more of the longest. The best statistical distribution to describe sunflecks and shadeflecks was the gamma distribution, which could provide the basis for the future development of a good model for sunfleck and shadefleck distributions.

  10. Genome-wide identification and characterization of aquaporin gene family in common bean (Phaseolus vulgaris L.).

    Science.gov (United States)

    Ariani, Andrea; Gepts, Paul

    2015-10-01

    Plant aquaporins are a large and diverse family of water channel proteins that are essential for several physiological processes in living organisms. Numerous studies have linked plant aquaporins with a plethora of processes, such as nutrient acquisition, CO2 transport, plant growth and development, and response to abiotic stresses. However, little is known about this protein family in common bean. Here, we present a genome-wide identification of the aquaporin gene family in common bean (Phaseolus vulgaris L.), a legume crop essential for human nutrition. We identified 41 full-length coding aquaporin sequences in the common bean genome, divided by phylogenetic analysis into five sub-families (PIPs, TIPs, NIPs, SIPs and XIPs). Residues determining substrate specificity of aquaporins (i.e., NPA motifs and ar/R selectivity filter) seem conserved between common bean and other plant species, allowing inference of substrate specificity for these proteins. Thanks to the availability of RNA-sequencing datasets, expression levels in different organs and in leaves of wild and domesticated bean accessions were evaluated. Three aquaporins (PvTIP1;1, PvPIP2;4 and PvPIP1;2) have the overall highest mean expressions, with PvTIP1;1 having the highest expression among all aquaporins. We performed an EST database mining to identify drought-responsive aquaporins in common bean. This analysis showed a significant increase in expression for PvTIP1;1 in drought stress conditions compared to well-watered environments. The pivotal role suggested for PvTIP1;1 in regulating water homeostasis and drought stress response in the common bean should be verified by further field experimentation under drought stress.

  11. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris).

    Science.gov (United States)

    Zuiderveen, Grady H; Padder, Bilal A; Kamfwa, Kelvin; Song, Qijian; Kelly, James D

    2016-01-01

    Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L.) caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS). Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481) included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL) for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL) population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219) tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  12. Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris.

    Directory of Open Access Journals (Sweden)

    Grady H Zuiderveen

    Full Text Available Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L. caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS. Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481 included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F4:6 recombinant inbred line (RIL population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219 tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthracnose resistance in Andean beans.

  13. Effect of Salt Stress on Three Green Bean (Phaseolus vulgaris L. Cultivars

    Directory of Open Access Journals (Sweden)

    Anna ASSIMAKOPOULOU

    2015-04-01

    Full Text Available Agriculture is increasingly forced to utilize marginal waters to meet its increasing demands, which in turn increases the risks of soil salinization and yield reduction in the arid and semi-arid areas of the Mediterranean basin. Given that the bean is an extremely salt sensitive species, the purpose of the present work was to study the effect of 0 and 75 mM sodium chloride (NaCl on leaf characteristics, growth, pod yield and ion accumulation of three green bean (Phaseolus vulgaris L. cultivars (‘Corallo Nano’, ‘Romano Bush Plaja’ and ‘Starazagorski’, widely used in Greece. Plants were grown in a greenhouse of Technological Educational Institute of Peloponnese in Messinia, Southern Greece, from April to June 2014, in hydroponics. The experimental design was the factorial completely randomized one with five replications; each replication consisted of the three plants grown on the same rockwool slab. The results of the majority of growth and yield parameters determined showed the superiority of ‘Corallo’ over ‘Romano’ whereas ‘Starazagorski’ tolerance was found to be intermediate. ‘Corallo’ tolerated NaCl salinity better due to its capacity for Na retention in the roots and maintaining appropriate K/Na and Ca/Na ratios, limiting the accumulation of toxic ions into actively growing shoots. The salt sensitivity of ‘Romano’ was related to its higher concentration of Na in the leaves and lower in the roots, to the greater decrease of the leaf number and leaf water content, as well as to the specific leaf area increase compared to the other two cultivars under saline conditions.

  14. Fortifi cation of white fl at bread with sprouted red kidney bean (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Kiruthika Viswanathan

    2014-03-01

    Full Text Available Background. Protein quantity in diet including the digestibility and bioavailability of protein is of importance to eradicate undernutrition in developing countries. Bread protein is an incomplete source as it lacks an essential amino acid lysine. When they are combined with other plant foods like pulses and legumes, they become a complete source of protein. Since bread is most common staple food the objective of this study is to fortify bread with legumes in order to increase the total protein content of bread to 13-15% which is required to meet at least 1/3rd of protein requirement of an adult recommended daily allowance. Material and methods. Fortifi cation of fl at bread was done by adding sprouted red kidney bean flour (Phaseolus vulgaris at 5, 15 and 25% to white fl our. The composite bread was analysed for crude protein and in vitro protein digestibility using the Kjeldahl and pepsin-pancreatin method. Results. The protein content of raw beans showed trivial increase on soaking for 17h and sprouting for 3 days. On the other hand, a remarkable increase was observed in protein digestibility i.e., 8% and 11% respectively. The protein content of control and composite breads increased gradually at 1% and protein digestibility decreased by 12% from control. This is due to the presence of dietary fi bers which bind with protein and inhibit its digestibility. Conclusion. The study infers that sprouting the beans for 72 h did not show any remarkable increase in protein content but a signifi cant increase in invitro protein digestibility was observed. Overall, breads made using 15% legume fl our was comparatively equal in protein content, with overall acceptable quality.

  15. Identification of novel drought-tolerant-associated SNPs in common bean (Phaseolus vulgaris

    Directory of Open Access Journals (Sweden)

    Emiliano eVillordo-Pineda

    2015-07-01

    Full Text Available Common bean (Phaseolus vulgaris L. is a leguminous in high demand for human nutrition and a veryimportant agricultural product. Production of common bean is constrained by environmental stressessuch as drought. Although conventional plant selection has been used to increase production yield andstress tolerance, drought tolerance selection based on phenotype is complicated by associatedphysiological, anatomical, cellular, biochemical and molecular changes. These changes are modulatedby differential gene expression. A common method to identify genes associated with phenotypes ofinterest is the characterization of Single Nucleotide Polymorphims (SNPs to link them to specificfunctions.In this work, we selected two drought-tolerant parental lines from Mesoamerica, Pinto Villa and PintoSaltillo. The parental lines were used to generate a population of 282 families (F 3:5 and characterizedby 169 SNPs . We associated the segregation of the molecular markers in our population withphenotypes including flowering time, physiological maturity, reproductive period, plant, seed and totalbiomass, reuse index, seed yield, weight of 100 seeds and harvest index in three cultivation cycles.We observed 83 SNPs with significant association (p < 0.0003 after Bonferroni correction with ourquantified phenotypes. Phenotypes most associated were days to flowering and seed biomass with 58and 44 associated SNPs respectively. Thirty-seven out of the 83 SNPs were annotated to a gene with apotential function related to drought tolerance or relevant molecular/biochemical functions. Some SNPssuch as SNP28 and SNP128 are related to starch biosynthesis, a common osmotic protector; andSNP18 is related to proline biosynthesis, another well-known osmotic protector.

  16. Preliminary Site Characterization Report, Rulsion Site, Colorado

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    This report is a summary of environmental information gathered during a review of the documents pertaining to Project Rulison and interviews with personnel who worked on the project. Project Rulison was part of Operation Plowshare (a program designed to explore peaceful uses for nuclear devices). The project consisted of detonating a 43-kiloton nuclear device on September 10, 1969, in western Colorado to stimulate natural gas production. Following the detonation, a reentry well was drilled and several gas production tests were conducted. The reentry well was shut-in after the last gas production test and was held in standby condition until the general cleanup was undertaken in 1972. A final cleanup was conducted after the emplacement and testing wells were plugged in 1976. However, some surface radiologic contamination resulted from decontamination of the drilling equipment and fallout from the gas flaring during drilling operations. With the exception of the drilling effluent pond, all surface contamination at the Rulison Site was removed during the cleanup operations. All mudpits and other excavations were backfilled, and both upper and lower drilling pads were leveled and dressed. This report provides information regarding known or suspected areas of contamination, previous cleanup activities, analytical results, a review of the regulatory status, the site`s physical environment, and future recommendations for Project Ruhson. Based on this research, several potential areas of contamination have been identified. These include the drilling effluent pond and mudpits used during drilling operations. In addition, contamination could migrate in the gas horizon.

  17. Birth outcomes in Colorado's undocumented immigrant population

    Directory of Open Access Journals (Sweden)

    Battaglia Catherine

    2005-10-01

    Full Text Available Abstract Background The birth outcomes of undocumented women have not been systematically studied on a large scale. The growing number of undocumented women giving birth in the United States has important implications for clinical care and public health policy. The objective of this study was to describe birth outcomes of undocumented immigrants in Colorado. Methods Retrospective descriptive study of singleton births to 5961 undocumented women using birth certificate data for 1998–1999. Results Undocumented mothers were younger, less educated, and more likely to be single. They had higher rates of anemia, were less likely to gain enough weight, and less likely to receive early prenatal care. They were much less likely to use alcohol or tobacco. Undocumented women had a lower rate of low birth weight (5.3% v 6.5%, P Conclusion Undocumented women have lower rates of preterm delivery and low birth weight infants, but higher rates of pregnancy related risk factors. Higher prevalence of some risk factors which are amenable to medical intervention reveals the need for improved prenatal care in this group.

  18. Geology of the Gypsum Gap quadrangle, Colorado

    Science.gov (United States)

    Cater, Fred W.

    1953-01-01

    The Gypsum Gap quadrangle is one eighteen 7 1/2-minute quadrangles covering the principal carnotite-producing area of southwestern Colorado. The geology of these quadrangles was mapped by the U.S. Geological Survey for the Atomic Energy Commission as part of a comparative study of carnotite deposits. The rocks exposed in the eighteen quadrangles consist of crystalline rocks of pre-Cambrian age and sedimentary rocks that range in age from late Paleozoic to Quaternary. Over much of the area the sedimentary rocks are flat lying, but in places the rocks are disrupted by high-angle faults, and northwest-trending folds. Conspicuous among the folds are large anticlines having cores of intrusive salt and gypsum. Most of the carnotite deposits are confined to the Salt Wash sandstone member of the Jurassic Morrison formation. Within this sandstone, most of the deposits are spottily distributed through a arcuate zone known as the "Uravan Mineral Belt". Individual deposits range in size from irregular masses containing only a few tons of ore to large, tabular masses containing many thousands of tons. The core consists largely of sandstone selectively impregnated and in part replaced by uranium and vanadium minerals. Most of the deposits appear to be related to certain sedimentary structures in sandstones of favorable composition.

  19. Geology of the Davis Mesa quadrangle, Colorado

    Science.gov (United States)

    Cater, Fred W.; Bryner, Leonid

    1953-01-01

    The Davis Mesa quadrangle is one of eighteen 7 1/2-minute quadrangles covering the principal carnotite-producing area of southwestern Colorado. The geology of these quadrangles was mapped by the U.S. Geological Survey for the Atomic Energy Commission as part of a comprehensive study of carnotite deposits. The rocks exposed in the eighteen quadrangles consist of crystalline rocks of pre-Cambrian age and sedimentary rocks that range in age from late Paleozoic to Quaternary. Over much of the area the sedimentary rocks are flat lying, but in places the rocks are disrupted by hih-angle faults, and northwest-trending folds. Conspicuous among the folds are large anticlines having cores of intrusive salt and gypsum. Most of the carnotite deposits are confined to the Salt Wash sandstone member of Jurassic Morrison formation. Within this sandstone, most of the deposits are spottily distributed through an arcuate zone known as "Uruvan Mineral Belt". Individual deposits range in size from irregular masses containing only a few tons of ore to large, tabular masses containing many thousands of tons. The ore consists largely of sandstone selectively impregnated and in part replaced by uranium and vanadium minerals. Most of the deposits appear to be related to certain sedimentary structures in sandstones of favorable composition.

  20. Geology of the Anderson Mesa quadrangle, Colorado

    Science.gov (United States)

    Cater, Fred W.; Withington, C.F.

    1953-01-01

    The Anderson Mesa quadrangle is one of the eighteen 7 1/2-minute quadrangles covering the principal carnotite-producing area of the southwestern Colorado. The geology of these quadrangles was mapped by the U.S. Geological Survey for the Atomic Energy Commission as part of a comprehensive study of carnotite deposits. The rocks exposed in the eighteenth quadrangles consist of crystalline rocks of pre-Cambrian age and sedimentary rocks that range in age from late Paleozoic to Quarternary. Over much of the area the sedimentary rocks are flat lying, but in places the rocks are disrupted by high-angle faults, and northwest-tending folds. Conspicuous among the folds are large anticlines having cores of intrusive slat and gypsum. Most of the carnotite deposits are confined to the Salt Wash sandstone member of the Jurassic Morrison formation. Within this sandstone, most of the deposits are spottily distributed through an arcuate zone known as the "Uravan Mineral Belt". Individual deposits range in size from irregular masses containing many thousands of tons. The ore consists of largely of sandstone selectively impregnated and in part replaced by uranium and vanadium minerals. Most of the deposits appear to be related to certain sedimentary structures in sandstones of favorable composition.