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Sample records for fork leonaradian age

  1. Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonaradian Age) Reservoirs, West Texas and New Mexico

    International Nuclear Information System (INIS)

    Lucia, F.J.; Ruppel, S.C.

    2001-01-01

    Characterization of cycle and facies architecture on lower Clear Fork and lowermost upper Clear Fork equivalent outcrops in Apache Canyon of Sierra Diablo was complete. The focus of detailed study in Apache Canyon has been the upper Clear Fork section because this interval contains the productive interval in South Wasson field, the preliminary subsurface study area. Parts of three high-frequency sequences (HFS), each 60 to 100 ft thick, are present on the south wall of Apache Canyon. HFS's display an upper-deepening or backstepping pattern associated with longer-term sea level rise. Each HFS is composed of upward-shallowing cycles whose thickness, facies composition, and continuity vary within and between HFS's

  2. Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonaradian Age) Reservoirs, West Texas and New Mexico, Semi-Annual; SEMIANNUAL

    International Nuclear Information System (INIS)

    Ruppel, Stephen C.; Jennings, James W.; Laubach, Stephen E.

    2001-01-01

    Outcrop studies include stratigraphic and petrophysical analysis. Analysis of the detailed sequence- and cycle-scale architecture of the Clear Fork reservoir-equivalent outcrops in Apache Canyon is nearly complete. This work reveals two high-frequency transgressive-regressive sequences (HFS) in the lower Clear Fork composite depositional sequence and three HFS in the basal middle Clear Fork composite depositional sequence. A 1,800-ft transect of 1-inch-diameter samples was collected from one cycle at the Apache Canyon outcrop. The transect was sampled with 5-ft spacing, but there were some gaps due to cover and cliff, resulting in 181 samples. Permeability, porosity, and grain density were measured, and the spatial statistics are being analyzed geostatistically

  3. Pitch Fork

    DEFF Research Database (Denmark)

    Williams, Peter Leslie; Overholt, Daniel

    2017-01-01

    Pitch Fork is a prototype of an alternate, actuated digital musical instrument (DMI). It uses 5 infra-red and 4 piezoelectric sensors to control an additive synthesis engine. Iron bars are used as the physical point of contact in interaction with the aim of using this materials natural acoustic p...... properties as a control signal for aspects of the digitally produced sound. This choice of material was also chosen to affect player experience. Sensor readings are relayed to a Macbook via an Arduino Mega. Mappings and audio output signal is carried out with Pure Data Extended....

  4. INTEGRATED OUTCROP AND SUBSURFACE STUDIES OF THE INTERWELL ENVIRONMENT OF CARBONATE RESERVOIRS: CLEAR FORK (LEONARDIAN-AGE) RESERVOIRS, WEST TEXAS AND NEW MEXICO

    Energy Technology Data Exchange (ETDEWEB)

    F. Jerry Lucia

    2002-01-31

    This is the final report of the project ''Integrated Outcrop and Subsurface Studies of the Interwell Environment of Carbonate Reservoirs: Clear Fork (Leonardian-Age) Reservoirs, West Texas and New Mexico'', Department of Energy contract no. DE-AC26-98BC15105 and is the third in a series of similar projects funded jointly by the U.S. Department of Energy and The University of Texas at Austin, Bureau of Economic Geology, Reservoir Characterization Research Laboratory for Carbonates. All three projects focus on the integration of outcrop and subsurface data for the purpose of developing improved methods for modeling petrophysical properties in the interwell environment. The first project, funded by contract no. DE-AC22-89BC14470, was a study of San Andres outcrops in the Algerita Escarpment, Guadalupe Mountains, Texas and New Mexico, and the Seminole San Andres reservoir, Permian Basin. This study established the basic concepts for constructing a reservoir model using sequence-stratigraphic principles and rock-fabric, petrophysical relationships. The second project, funded by contract no. DE-AC22-93BC14895, was a study of Grayburg outcrops in the Brokeoff Mountains, New Mexico, and the South Cowden Grayburg reservoir, Permian Basin. This study developed a sequence-stratigraphic succession for the Grayburg and improved methods for locating remaining hydrocarbons in carbonate ramp reservoirs. The current study is of the Clear Fork Group in Apache Canyon, Sierra Diablo Mountains, West Texas, and the South Wasson Clear Fork reservoir, Permian Basin. The focus was on scales of heterogeneity, imaging high- and low-permeability layers, and the impact of fractures on reservoir performance. In this study (1) the Clear Fork cycle stratigraphy is defined, (2) important scales of petrophysical variability are confirmed, (3) a unique rock-fabric, petrophysical relationship is defined, (4) a porosity method for correlating high-frequency cycles and defining rock

  5. Eukaryotic DNA Replication Fork.

    Science.gov (United States)

    Burgers, Peter M J; Kunkel, Thomas A

    2017-06-20

    This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed. The preponderance of evidence supports a model in which DNA polymerase ε (Pol ε) carries out the bulk of leading strand DNA synthesis at an undisturbed replication fork. DNA polymerases α and δ carry out the initiation of Okazaki fragment synthesis and its elongation and maturation, respectively. This review also discusses alternative proposals, including cellular processes during which alternative forks may be utilized, and new biochemical studies with purified proteins that are aimed at reconstituting leading and lagging strand DNA synthesis separately and as an integrated replication fork.

  6. Economic fork lifters

    Energy Technology Data Exchange (ETDEWEB)

    Roedig, W

    1981-01-01

    Increased energy costs attribute new interest to the choice between electric fork lifters and fork lifters driven by combustion engines. The advantages and shortcomings of the two drive systems are listed. As previous cost-comparisons are no longer up-to-date a new comparison is made in order to find out which of the three types works most economically: electric diesel- or fuel gas driven fork lifters. The comparison is based on a one-year-operation with a load capacity of 1,5 to 5 tons under normal stress conditions. The following parameters are compared: sum of investments, depreciation, interest rates, fixed costs per annum and per hour of operation. Variable costs like: repair costs, costs for replacement parts, energy cost and total cost. The three-wheeled electric fork lifter has proved to be the most economic one followed by the diesel-driven fork lifter, the four-wheel electric fork lifter and the fuel-gas driven fork lifter.

  7. SC tuning fork

    CERN Document Server

    The tuning fork used to modulate the radiofrequency system of the synchro cyclotron (SC) from 1957 to 1973. This piece is an unused spare part. The SC was the 1st accelerator built at CERN. It operated from August 1957 until it was closed down at the end of 1990. In the SC the magnetic field did not change with time, and the particles were accelerated in successive pulses by a radiofrequency voltage of some 20kV which varied in frequency as they spiraled outwards towards the extraction radius. The frequency varied from 30MHz to about 17Mz in each pulse. The tuning fork vibrated at 55MHz in vacuum in an enclosure which formed a variable capacitor in the tuning circuit of the RF system, allowing the RF to vary over the appropriate range to accelerate protons from the centre of the macine up to 600Mev at extraction radius. In operation the tips of the tuning fork blade had an amplitude of movement of over 1 cm. The SC accelerator underwent extensive improvements from 1973 to 1975, including the installation of a...

  8. The forked flap repair for hypospadias

    Directory of Open Access Journals (Sweden)

    Anil Chadha

    2012-01-01

    Full Text Available Context: Despite the abundance of techniques for the repair of Hypospadias, its problems still persist and a satisfactory design to correct the penile curvature with the formation of neourethra from the native urethral tissue or genital or extragenital tissues, with minimal postoperative complications has yet to evolve. Aim: Persisting with such an endeavor, a new technique for the repair of distal and midpenile hypospadias is described. Materials and Methods: The study has been done in 70 cases over the past 11 years. The "Forked-Flap" repair is a single stage method for the repair of such Hypospadias with chordee. It takes advantage of the rich vascular communication at the corona and capitalizes on the established reliability of the meatal based flip-flap. The repair achieves straightening of the curvature of the penis by complete excision of chordee tissue from the ventral surface of the penis beneath the urethral plate. The urethra is reconstructed using the native plate with forked flap extensions and genital tissue relying on the concept of meatal based flaps. Water proofing by dartos tissue and reinforcement by Nesbit′s prepucial tissue transfer completes the one stage procedure. Statistical Analysis: An analysis of 70 cases of this single stage technique of repair of penile hypospadias with chordee, operated at 3 to 5 years of age over the past 11 years is presented. Results and Conclusion: The Forked Flap gives comparable and replicable results; except for a urethrocutaneous fistula rate of 4% no other complications were observed.

  9. Did mosasaurs have forked tongues?

    NARCIS (Netherlands)

    Schulp, Anne S.; Mulder, E. W. A.; Schwenk, K.

    Ever since the first mosasaur restorations were published, these extinct marine reptiles have been pictured with either notched, forked or undivided tongues. Here, we present an overview of existing iconography, a review of the previous literature, and we discuss how best to reconstruct tongue form

  10. South Fork Holston River basin 1988 biomonitoring

    Energy Technology Data Exchange (ETDEWEB)

    Saylor, C.F.; Ahlstedt, S.A.

    1990-06-01

    There is concern over the effects of shifts in land use use practices on the aquatic fauna of streams in the South Fork Holston River basin in northwestern North Carolina and southwestern Virginia. Trout reproduction has noticeably declined in the Watauga River subbasin. The Watauga River and Elk River subbasins have been subjected to commercial and resort development. The Middle fork Holston River and the upper South Fork Holston River subbasins have been affected by agricultural and mining activities, respectively (Cox, 1986). To aid reclamation and management of the South Fork Holston basin, Tennessee Valley Authority (TVA) biologists conducted biomonitoring--including index of biotic integrity and macroinvertebrate sampling--on the Middle Fork Holston, South Fork Holston, Watauga, and Elk Rivers to assess cumulative impairment related to changes in habitat and pollutant loading in these subbasins. Biomonitoring can detect environmental degradation, help document problem areas, and assist in development of strategies for managing water quality. This report discusses the methods and materials and results of the biomonitoring of South Fork Holston River Basin. 13 refs., 5 figs., 12 tabs.

  11. Burnup verification using the FORK measurement system

    International Nuclear Information System (INIS)

    Ewing, R.I.

    1994-01-01

    Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. The FORK measurement system, designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program, has been used to verify reactor site records for burnup and cooling time for many years. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. This report deals with the application of the FORK system to burnup credit operations based on measurements performed on spent fuel assemblies at the Oconee Nuclear Station of Duke Power Company

  12. Code Forking, Governance, and Sustainability in Open Source Software

    OpenAIRE

    Juho Lindman; Linus Nyman

    2013-01-01

    The right to fork open source code is at the core of open source licensing. All open source licenses grant the right to fork their code, that is to start a new development effort using an existing code as its base. Thus, code forking represents the single greatest tool available for guaranteeing sustainability in open source software. In addition to bolstering program sustainability, code forking directly affects the governance of open source initiatives. Forking, and even the mere possibilit...

  13. Henrys Fork near Ashton, ID (YHEN)

    Data.gov (United States)

    Department of the Interior — Henrys Fork near Ashton, Idaho (YHEN) Sample Collection: Samples were collected near the USGS stream gage 13046000 (Latitude 44°04'11", Longitude 111°30'38" NAD83)....

  14. FBH1 Catalyzes Regression of Stalled Replication Forks

    Directory of Open Access Journals (Sweden)

    Kasper Fugger

    2015-03-01

    Full Text Available DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here, we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replication forks.

  15. Pyrimidine dimers block simian virus 40 replication forks

    International Nuclear Information System (INIS)

    Berger, C.A.; Edenberg, H.J.

    1986-01-01

    UV light produces lesions, predominantly pyrimidine dimers, which inhibit DNA replication in mammalian cells. The mechanism of inhibition is controversial: is synthesis of a daughter strand halted at a lesion while the replication fork moves on and reinitiates downstream, or is fork progression itself blocked for some time at the site of a lesion? We directly addressed this question by using electron microscopy to examine the distances of replication forks from the origin in unirradiated and UV-irradiated simian virus 40 chromosomes. If UV lesions block replication fork progression, the forks should be asymmetrically located in a large fraction of the irradiated molecules; if replication forks move rapidly past lesions, the forks should be symmetrically located. A large fraction of the simian virus 40 replication forks in irradiated molecules were asymmetrically located, demonstrating that UV lesions present at the frequency of pyrimidine dimers block replication forks. As a mechanism for this fork blockage, we propose that polymerization of the leading strand makes a significant contribution to the energetics of fork movement, so any lesion in the template for the leading strand which blocks polymerization should also block fork movement

  16. DNA fork displacement rates in human cells

    International Nuclear Information System (INIS)

    Kapp, L.N.; Painter, R.B.

    1981-01-01

    DNA fork displacement rates were measured in 20 human cell lines by a bromodeoxyuridine-313 nm photolysis technique. Cell lines included representatives of normal diploid, Fanconi's anemia, ataxia telangiectasia, xeroderma pigmentosum, trisomy-21 and several transformed lines. The average value for all the cell lines was 0.53 +- 0.08 μm/min. The average value for individual cell lines, however, displayed a 30% variation. Less than 10% of variation in the fork displacement rate appears to be due to the experimental technique; the remainder is probably due to true variation among the cell types and to culture conditions. (Auth.)

  17. DNA fork displacement rates in human cells

    Energy Technology Data Exchange (ETDEWEB)

    Kapp, L.N.; Painter, R.B. (California Univ., San Francisco (USA). Lab. of Radiobiology)

    1981-11-27

    DNA fork displacement rates were measured in 20 human cell lines by a bromodeoxyuridine-313 nm photolysis technique. Cell lines included representatives of normal diploid, Fanconi's anemia, ataxia telangiectasia, xeroderma pigmentosum, trisomy-21 and several transformed lines. The average value for all the cell lines was 0.53 +- 0.08 ..mu..m/min. The average value for individual cell lines, however, displayed a 30% variation. Less than 10% of variation in the fork displacement rate appears to be due to the experimental technique; the remainder is probably due to true variation among the cell types and to culture conditions.

  18. Development of the Pintle Release Fork Mechanism

    International Nuclear Information System (INIS)

    BOGER, R.M.; DALE, R.

    1999-01-01

    An improved method of attachment of the pintle to the piston in the universal sampler is being developed. The mechanism utilizes a forked release disk which captures two balls in a cavity formed by a hole in the piston and a groove in the pintle rod

  19. Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery.

    Science.gov (United States)

    Murphy, Anar K; Fitzgerald, Michael; Ro, Teresa; Kim, Jee Hyun; Rabinowitsch, Ariana I; Chowdhury, Dipanjan; Schildkraut, Carl L; Borowiec, James A

    2014-08-18

    Phosphorylation of replication protein A (RPA) by Cdk2 and the checkpoint kinase ATR (ATM and Rad3 related) during replication fork stalling stabilizes the replisome, but how these modifications safeguard the fork is not understood. To address this question, we used single-molecule fiber analysis in cells expressing a phosphorylation-defective RPA2 subunit or lacking phosphatase activity toward RPA2. Deregulation of RPA phosphorylation reduced synthesis at forks both during replication stress and recovery from stress. The ability of phosphorylated RPA to stimulate fork recovery is mediated through the PALB2 tumor suppressor protein. RPA phosphorylation increased localization of PALB2 and BRCA2 to RPA-bound nuclear foci in cells experiencing replication stress. Phosphorylated RPA also stimulated recruitment of PALB2 to single-strand deoxyribonucleic acid (DNA) in a cell-free system. Expression of mutant RPA2 or loss of PALB2 expression led to significant DNA damage after replication stress, a defect accentuated by poly-ADP (adenosine diphosphate) ribose polymerase inhibitors. These data demonstrate that phosphorylated RPA recruits repair factors to stalled forks, thereby enhancing fork integrity during replication stress. © 2014 Murphy et al.

  20. Homologous Recombination as a Replication Fork Escort: Fork-Protection and Recovery

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    2012-12-01

    Full Text Available Homologous recombination is a universal mechanism that allows DNA repair and ensures the efficiency of DNA replication. The substrate initiating the process of homologous recombination is a single-stranded DNA that promotes a strand exchange reaction resulting in a genetic exchange that promotes genetic diversity and DNA repair. The molecular mechanisms by which homologous recombination repairs a double-strand break have been extensively studied and are now well characterized. However, the mechanisms by which homologous recombination contribute to DNA replication in eukaryotes remains poorly understood. Studies in bacteria have identified multiple roles for the machinery of homologous recombination at replication forks. Here, we review our understanding of the molecular pathways involving the homologous recombination machinery to support the robustness of DNA replication. In addition to its role in fork-recovery and in rebuilding a functional replication fork apparatus, homologous recombination may also act as a fork-protection mechanism. We discuss that some of the fork-escort functions of homologous recombination might be achieved by loading of the recombination machinery at inactivated forks without a need for a strand exchange step; as well as the consequence of such a model for the stability of eukaryotic genomes.

  1. Code Forking, Governance, and Sustainability in Open Source Software

    Directory of Open Access Journals (Sweden)

    Juho Lindman

    2013-01-01

    Full Text Available The right to fork open source code is at the core of open source licensing. All open source licenses grant the right to fork their code, that is to start a new development effort using an existing code as its base. Thus, code forking represents the single greatest tool available for guaranteeing sustainability in open source software. In addition to bolstering program sustainability, code forking directly affects the governance of open source initiatives. Forking, and even the mere possibility of forking code, affects the governance and sustainability of open source initiatives on three distinct levels: software, community, and ecosystem. On the software level, the right to fork makes planned obsolescence, versioning, vendor lock-in, end-of-support issues, and similar initiatives all but impossible to implement. On the community level, forking impacts both sustainability and governance through the power it grants the community to safeguard against unfavourable actions by corporations or project leaders. On the business-ecosystem level forking can serve as a catalyst for innovation while simultaneously promoting better quality software through natural selection. Thus, forking helps keep open source initiatives relevant and presents opportunities for the development and commercialization of current and abandoned programs.

  2. Structural design of a composite bicycle fork

    International Nuclear Information System (INIS)

    Baldissera, Paolo; Delprete, Cristiana

    2014-01-01

    Highlights: • Case study about composite bicycle fork design. • Special requirements for a Student Team project. • FE model to evaluate stiffness, strength and potential failure modes. • Comparison of two manufacturing approaches. • FE model stiffness validation on the manufactured fork. - Abstract: Despite the wide literature on the mechanical behaviour of carbon/epoxy composites, it is rare to find practical methodological approaches in finite element design of structural components made by laminate layup. Through the case study of a special bicycle fork needed in a Student Team prototype, this paper proposes a simplified methodology as starting point for educational and manufacturing purposes. In order to compare two manufacturing solutions in terms of stiffness, strength and failure mode, a numerical model was implemented. Since the project requirements imposed to avoid standard destructive testing, the model validation was based on a posteriori linear stiffness comparison with the manufactured component. The slight discrepancies between experimental and numerical results were discussed in order to check their origin and to assess the reliability of the model. The overall methodology, even if complain with only a part of the safety standard requirements, shows to be reliable enough and can be the basis for further extension and refinement

  3. Regulation of replication fork progression through histone supply and demand

    DEFF Research Database (Denmark)

    Groth, Anja; Corpet, Armelle; Cook, Adam J L

    2007-01-01

    DNA replication in eukaryotes requires nucleosome disruption ahead of the replication fork and reassembly behind. An unresolved issue concerns how histone dynamics are coordinated with fork progression to maintain chromosomal stability. Here, we characterize a complex in which the human histone c...... progression and histone supply and demand.......1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin. We propose that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork...

  4. Stalled replication forks generate a distinct mutational signature in yeast

    DEFF Research Database (Denmark)

    Larsen, Nicolai B.; Liberti, Sascha E.; Vogel, Ivan

    2017-01-01

    Proliferating cells acquire genome alterations during the act of DNA replication. This leads to mutation accumulation and somatic cell mosaicism in multicellular organisms, and is also implicated as an underlying cause of aging and tumorigenesis. The molecular mechanisms of DNA replication...... Escherichia coli Tus/Ter complex) engineered into the yeast genome. We demonstrate that transient stalling at this barrier induces a distinct pattern of genome rearrangements in the newly replicated region behind the stalled fork, which primarily consist of localized losses and duplications of DNA sequences....... These genetic alterations arise through the aberrant repair of a single-stranded DNA gap, in a process that is dependent on Exo1- and Shu1-dependent homologous recombination repair (HRR). Furthermore, aberrant processing of HRR intermediates, and elevated HRR-associated mutagenesis, is detectable in a yeast...

  5. The ophiolitic North Fork terrane in the Salmon River region, central Klamath Mountains, California

    Science.gov (United States)

    Ando, C.J.; Irwin, W.P.; Jones, D.L.; Saleeby, J.B.

    1983-01-01

    The North Fork terrane is an assemblage of ophiolitic and other oceanic volcanic and sedimentary rocks that has been internally imbricated and folded. The ophiolitic rocks form a north-trending belt through the central part of the region and consist of a disrupted sequence of homogeneous gabbro, diabase, massive to pillowed basalt, and interleaved tectonitic harzburgite. U-Pb zircon age data on a plagiogranite pod from the gabbroic unit indicate that at least this part of the igneous sequence is late Paleozoic in age.The ophiolitic belt is flanked on either side by mafic volcanic and volcaniclastic rocks, limestone, bedded chert, and argillite. Most of the chert is Triassic, including much of Late Triassic age, but chert with uncertain stratigraphic relations at one locality is Permian. The strata flanking the east side of the ophiolitic belt face eastward, and depositional contacts between units are for the most part preserved. The strata on the west side of the ophiolitic belt are more highly disrupted than those on the east side, contain chert-argillite melange, and have unproven stratigraphic relation to either the ophiolitic rocks or the eastern strata.Rocks of the North Fork terrane do not show widespread evidence of penetrative deformation at elevated temperatures, except an early tectonitic fabric in the harzburgite. Slip-fiber foliation in serpentinite, phacoidal foliation in chert and mafic rocks, scaly foliation in argillite, and mesoscopic folds in bedded chert are consistent with an interpretation of large-scale anti-formal folding of the terrane about a north-south hinge found along the ophiolitic belt, but other structural interpretations are tenable. The age of folding of North Fork rocks is constrained by the involvement of Triassic and younger cherts and crosscutting Late Jurassic plutons. Deformation in the North Fork terrane must have spanned a short period of time because the terrane is bounded structurally above and below by Middle or Late

  6. Intestinal Fork Head Regulates Nutrient Absorption and Promotes Longevity

    Directory of Open Access Journals (Sweden)

    Ekin Bolukbasi

    2017-10-01

    Full Text Available Reduced activity of nutrient-sensing signaling networks can extend organismal lifespan, yet the underlying biology remains unclear. We show that the anti-aging effects of rapamycin and reduced intestinal insulin/insulin growth factor (IGF signaling (IIS require the Drosophila FoxA transcription factor homolog Fork Head (FKH. Intestinal FKH induction extends lifespan, highlighting a role for the gut. FKH binds to and is phosphorylated by AKT and Target of Rapamycin. Gut-specific FKH upregulation improves gut barrier function in aged flies. Additionally, it increases the expression of nutrient transporters, as does lowered IIS. Evolutionary conservation of this effect of lowered IIS is suggested by the upregulation of related nutrient transporters in insulin receptor substrate 1 knockout mouse intestine. Our study highlights a critical role played by FKH in the gut in mediating anti-aging effects of reduced IIS. Malnutrition caused by poor intestinal absorption is a major problem in the elderly, and a better understanding of the mechanisms involved will have important therapeutic implications for human aging.

  7. Development of Tuning Fork Based Probes for Atomic Force Microscopy

    Science.gov (United States)

    Jalilian, Romaneh; Yazdanpanah, Mehdi M.; Torrez, Neil; Alizadeh, Amirali; Askari, Davood

    2014-03-01

    This article reports on the development of tuning fork-based AFM/STM probes in NaugaNeedles LLC for use in atomic force microscopy. These probes can be mounted on different carriers per customers' request. (e.g., RHK carrier, Omicron carrier, and tuning fork on a Sapphire disk). We are able to design and engineer tuning forks on any type of carrier used in the market. We can attach three types of tips on the edge of a tuning fork prong (i.e., growing Ag2Ga nanoneedles at any arbitrary angle, cantilever of AFM tip, and tungsten wire) with lengths from 100-500 μm. The nanoneedle is located vertical to the fork. Using a suitable insulation and metallic coating, we can make QPlus sensors that can detect tunneling current during the AFM scan. To make Qplus sensors, the entire quartz fork will be coated with an insulating material, before attaching the nanoneedle. Then, the top edge of one prong is coated with a thin layer of conductive metal and the nanoneedle is attached to the fork end of the metal coated prong. The metal coating provides electrical connection to the tip for tunneling current readout and to the electrodes and used to read the QPlus current. Since the amount of mass added to the fork is minimal, the resonance frequency spectrum does not change and still remains around 32.6 KHz and the Q factor is around 1,200 in ambient condition. These probes can enhance the performance of tuning fork based atomic microscopy.

  8. Chemical and biological sensing using tuning forks

    Science.gov (United States)

    Tao, Nongjian; Boussaad, Salah

    2012-07-10

    A device for sensing a chemical analyte is disclosed. The device is comprised of a vibrating structure having first and second surfaces and having an associated resonant frequency and a wire coupled between the first and second surfaces of the vibrating structure, wherein the analyte interacts with the wire and causes a change in the resonant frequency of the vibrating structure. The vibrating structure can include a tuning fork. The vibrating structure can be comprised of quartz. The wire can be comprised of polymer. A plurality of vibrating structures are arranged in an array to increase confidence by promoting a redundancy of measurement or to detect a plurality of chemical analytes. A method of making a device for sensing a chemical analyte is also disclosed.

  9. North Fork Feather River Erosion Control Program

    International Nuclear Information System (INIS)

    Harrison, L.

    1991-01-01

    PG and E, an investor owned gas and electric utility serving northern and central California, has been engaged since 1984 in the development and implementation of a regional erosion control program for the 954 square mile northern Sierra Nevada watersheed of the East Branch of the North Fork Feather River in Plumas County, California. PG and E entered into an agreement with 13 governmental agencies and a number of private landowners using Coordinated Resource Management and Planning: to cooperatively develop, fund and implement the program. The group has completed several field projects and has a number of additional projects in various stages of development. This paper reports that the program provides multiple environmental and economic benefits including reduction of soil erosion and sedimentation, improved fisheries, enhancement of riparian habitat, increased land values, improved recreation opportunities, and preservation of watershed resources

  10. Spent-fuel verification with the Los Alamos fork detector

    International Nuclear Information System (INIS)

    Rinard, P.M.; Bosler, G.E.

    1987-01-01

    The Los Alamos fork detector for the verification of spent-fuel assemblies has generated precise, reproducible data. The data analyses have now evolved to the point of placing tight restrictions on a diverter's actions

  11. Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork

    OpenAIRE

    Rapp, Jordan B.; Ansbach, Alison B.; Noguchi, Chiaki; Noguchi, Eishi

    2009-01-01

    Replication of chromosomes involves a variety of replication proteins including DNA polymerases, DNA helicases, and other accessory factors. Many of these proteins are known to localize at replication forks and travel with them as components of the replisome complex. Other proteins do not move with replication forks but still play an essential role in DNA replication. Therefore, in order to understand the mechanisms of DNA replication and its controls, it is important to examine localization ...

  12. Uncertainty Quantification of Fork Detector Measurements from Spent Fuel Loading Campaigns

    International Nuclear Information System (INIS)

    Vaccaro, S.; De Baere, P.; Schwalbach, P.; Gauld, I.; Hu, J.

    2015-01-01

    With increasing activities at the end of the fuel cycle, the requirements for the verification of spent nuclear fuel for safeguards purposes are continuously growing. In the European Union we are experiencing a dramatic increase in the number of cask loadings for interim dry storage. This is caused by the progressive shut-down of reactors, related to facility ageing but also due to politically motivated phase-out of nuclear power. On the other hand there are advanced plans for the construction of encapsulation plants and geological repositories. The cask loading or the encapsulation process will provide the last occasion to verify the spent fuel assemblies. In this context, Euratom and the US DOE have carried out a critical review of the widely used Fork measurements method of irradiated assemblies. The Nuclear Safeguards directorates of the European Commission's Directorate General for Energy and Oak Ridge National Laboratory have collaborated to improve the Fork data evaluation process and simplify its use for inspection applications. Within the Commission's standard data evaluation package CRISP, we included a SCALE/ORIGEN-based irradiation and depletion simulation of the measured assembly and modelled the fork transfer function to calculate expected count rates based on operator's declarations. The complete acquisition and evaluation process has been automated to compare expected (calculated) with measured count rates. This approach allows a physics-based improvement of the data review and evaluation process. At the same time the new method provides the means for better measurement uncertainty quantification. The present paper will address the implications of the combined approach involving measured and simulated data to the quantification of measurement uncertainty and the consequences of these uncertainties in the possible use of the Fork detector as a partial defect detection method. (author)

  13. Force regulated dynamics of RPA on a DNA fork.

    Science.gov (United States)

    Kemmerich, Felix E; Daldrop, Peter; Pinto, Cosimo; Levikova, Maryna; Cejka, Petr; Seidel, Ralf

    2016-07-08

    Replication protein A (RPA) is a single-stranded DNA binding protein, involved in most aspects of eukaryotic DNA metabolism. Here, we study the behavior of RPA on a DNA substrate that mimics a replication fork. Using magnetic tweezers we show that both yeast and human RPA can open forked DNA when sufficient external tension is applied. In contrast, at low force, RPA becomes rapidly displaced by the rehybridization of the DNA fork. This process appears to be governed by the binding or the release of an RPA microdomain (toehold) of only few base-pairs length. This gives rise to an extremely rapid exchange dynamics of RPA at the fork. Fork rezipping rates reach up to hundreds of base-pairs per second, being orders of magnitude faster than RPA dissociation from ssDNA alone. Additionally, we show that RPA undergoes diffusive motion on ssDNA, such that it can be pushed over long distances by a rezipping fork. Generally the behavior of both human and yeast RPA homologs is very similar. However, in contrast to yeast RPA, the dissociation of human RPA from ssDNA is greatly reduced at low Mg(2+) concentrations, such that human RPA can melt DNA in absence of force. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  14. Aerial gamma ray and magnetic survey: Minnesota Project, Grand Forks quadrangle of Minnesota/North Dakota. Final report

    International Nuclear Information System (INIS)

    1979-12-01

    The Grand Forks 1:250,000 scale quadrangle of Minnesota and North Dakota is almost everywhere covered with Wisconsin age glacial deposits (drift, lake sediments, etc.) of variable thickness. Where exposed, bedrock is Late Cretaceous age marine deposits. There are no uranium deposits (or occurrences) known within the quadrangle. Seventy-eight (78) groups of uranium samples were defined as anomalies and are discussed briefly in this report. None of them are considered significant

  15. Disruption of PCNA-lamins A/C interactions by prelamin A induces DNA replication fork stalling.

    Science.gov (United States)

    Cobb, Andrew M; Murray, Thomas V; Warren, Derek T; Liu, Yiwen; Shanahan, Catherine M

    2016-09-02

    The accumulation of prelamin A is linked to disruption of cellular homeostasis, tissue degeneration and aging. Its expression is implicated in compromised genome stability and increased levels of DNA damage, but to date there is no complete explanation for how prelamin A exerts its toxic effects. As the nuclear lamina is important for DNA replication we wanted to investigate the relationship between prelamin A expression and DNA replication fork stability. In this study we report that the expression of prelamin A in U2OS cells induced both mono-ubiquitination of proliferating cell nuclear antigen (PCNA) and subsequent induction of Pol η, two hallmarks of DNA replication fork stalling. Immunofluorescence microscopy revealed that cells expressing prelamin A presented with high levels of colocalisation between PCNA and γH2AX, indicating collapse of stalled DNA replication forks into DNA double-strand breaks. Subsequent protein-protein interaction assays showed prelamin A interacted with PCNA and that its presence mitigated interactions between PCNA and the mature nuclear lamina. Thus, we propose that the cytotoxicity of prelamin A arises in part, from it actively competing against mature lamin A to bind PCNA and that this destabilises DNA replication to induce fork stalling which in turn contributes to genomic instability.

  16. Advancing the Fork detector for quantitative spent nuclear fuel verification

    Science.gov (United States)

    Vaccaro, S.; Gauld, I. C.; Hu, J.; De Baere, P.; Peterson, J.; Schwalbach, P.; Smejkal, A.; Tomanin, A.; Sjöland, A.; Tobin, S.; Wiarda, D.

    2018-04-01

    The Fork detector is widely used by the safeguards inspectorate of the European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) to verify spent nuclear fuel. Fork measurements are routinely performed for safeguards prior to dry storage cask loading. Additionally, spent fuel verification will be required at the facilities where encapsulation is performed for acceptance in the final repositories planned in Sweden and Finland. The use of the Fork detector as a quantitative instrument has not been prevalent due to the complexity of correlating the measured neutron and gamma ray signals with fuel inventories and operator declarations. A spent fuel data analysis module based on the ORIGEN burnup code was recently implemented to provide automated real-time analysis of Fork detector data. This module allows quantitative predictions of expected neutron count rates and gamma units as measured by the Fork detectors using safeguards declarations and available reactor operating data. This paper describes field testing of the Fork data analysis module using data acquired from 339 assemblies measured during routine dry cask loading inspection campaigns in Europe. Assemblies include both uranium oxide and mixed-oxide fuel assemblies. More recent measurements of 50 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel are also analyzed. An evaluation of uncertainties in the Fork measurement data is performed to quantify the ability of the data analysis module to verify operator declarations and to develop quantitative go/no-go criteria for safeguards verification measurements during cask loading or encapsulation operations. The goal of this approach is to provide safeguards inspectors with reliable real-time data analysis tools to rapidly identify discrepancies in operator declarations and to detect potential partial defects in spent fuel assemblies with improved reliability and minimal false positive alarms

  17. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe.

    Science.gov (United States)

    Margalef, Pol; Kotsantis, Panagiotis; Borel, Valerie; Bellelli, Roberto; Panier, Stephanie; Boulton, Simon J

    2018-01-25

    Telomere maintenance critically depends on the distinct activities of telomerase, which adds telomeric repeats to solve the end replication problem, and RTEL1, which dismantles DNA secondary structures at telomeres to facilitate replisome progression. Here, we establish that reversed replication forks are a pathological substrate for telomerase and the source of telomere catastrophe in Rtel1 -/- cells. Inhibiting telomerase recruitment to telomeres, but not its activity, or blocking replication fork reversal through PARP1 inhibition or depleting UBC13 or ZRANB3 prevents the rapid accumulation of dysfunctional telomeres in RTEL1-deficient cells. In this context, we establish that telomerase binding to reversed replication forks inhibits telomere replication, which can be mimicked by preventing replication fork restart through depletion of RECQ1 or PARG. Our results lead us to propose that telomerase inappropriately binds to and inhibits restart of reversed replication forks within telomeres, which compromises replication and leads to critically short telomeres. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  18. 77 FR 39675 - Wallowa-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining

    Science.gov (United States)

    2012-07-05

    ...-Whitman National Forest, Baker County, OR; North Fork Burnt River Mining AGENCY: Forest Service, USDA... North Fork Burnt River Mining Record of Decision will replace and supercede the 2004 North Fork Burnt River Mining Record of Decision only where necessary to address the inadequacies identified by the court...

  19. Comparison of the yields of downstream migrant salmonids before and after logging and road construction on the South Fork Caspar Creek, Mendocino County

    Science.gov (United States)

    David S. Graves; James W. Burns

    1970-01-01

    Yields of juvenile steelhead rainbow trout Salmo gairdnerii gairdnerii) and silver salmon (Oncorhynchus kisutch) emigrants were compared in South Fork Caspar Creek, a small coastal stream in Mendocino County, California, before and after construction of a logging road along the stream in the summer of 1967. Numbers, lengths, and age class structures were compared....

  20. Forked and Integrated Variants In An Open-Source Firmware Project

    DEFF Research Database (Denmark)

    Stanciulescu, Stefan; Schulze, Sandro; Wasowski, Andrzej

    2015-01-01

    and interactive source management platforms such as Github. We study advantages and disadvantages of forking using the case of Marlin, an open source firmware for 3D printers. We find that many problems and advantages of cloning do translate to forking. Interestingly, the Marlin community uses both forking......Code cloning has been reported both on small (code fragments) and large (entire projects) scale. Cloning-in-the-large, or forking, is gaining ground as a reuse mechanism thanks to availability of better tools for maintaining forked project variants, hereunder distributed version control systems...

  1. A Transimpedance Amplifier for Remotely Located Quartz Tuning Forks

    OpenAIRE

    Kleinbaum, Ethan; Csathy, Gabor

    2012-01-01

    The cable capacitance in cryogenic and high vacuum applications of quartz tuning forks imposes severe constraints on the bandwidth and noise performance of the measurement. We present a single stage low noise transimpedance amplifier with a bandwidth exceeding 1 MHz and provide an in-depth analysis of the dependence of the amplifier parameters on the cable capacitance.

  2. Note: a transimpedance amplifier for remotely located quartz tuning forks.

    Science.gov (United States)

    Kleinbaum, Ethan; Csáthy, Gábor A

    2012-12-01

    The cable capacitance in cryogenic and high vacuum applications of quartz tuning forks imposes severe constraints on the bandwidth and noise performance of the measurement. We present a single stage low noise transimpedance amplifier with a bandwidth exceeding 1 MHz and provide an in-depth analysis of the dependence of the amplifier parameters on the cable capacitance.

  3. Flood disaster preparedness: a retrospect from Grand Forks, North Dakota.

    Science.gov (United States)

    Siders, C; Jacobson, R

    1998-01-01

    Natural disasters often come without warning. The clinical, financial, and business risks can be enormous. Grand Forks' (ND) healthcare systems experienced a flooding disaster of unprecedented proportions in April of 1997. Planned and practiced disaster and evacuation procedures can significantly reduce a healthcare facilities' risk to life, health, and safety. This article retrospectively analyzes disaster preparation and the complete evacuation of the facilities' patients.

  4. Assembly of Slx4 signaling complexes behind DNA replication forks.

    Science.gov (United States)

    Balint, Attila; Kim, TaeHyung; Gallo, David; Cussiol, Jose Renato; Bastos de Oliveira, Francisco M; Yimit, Askar; Ou, Jiongwen; Nakato, Ryuichiro; Gurevich, Alexey; Shirahige, Katsuhiko; Smolka, Marcus B; Zhang, Zhaolei; Brown, Grant W

    2015-08-13

    Obstructions to replication fork progression, referred to collectively as DNA replication stress, challenge genome stability. In Saccharomyces cerevisiae, cells lacking RTT107 or SLX4 show genome instability and sensitivity to DNA replication stress and are defective in the completion of DNA replication during recovery from replication stress. We demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery. Slx4 complex formation is nucleated by Mec1 phosphorylation of histone H2A, which is recognized by the constitutive Slx4 binding partner Rtt107. Slx4 is essential for recruiting the Mec1 activator Dpb11 behind stressed replication forks, and Slx4 complexes are important for full activity of Mec1. We propose that Slx4 complexes promote robust checkpoint signaling by Mec1 by stably recruiting Dpb11 within a discrete domain behind the replication fork, during DNA replication stress. © 2015 The Authors.

  5. Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability.

    Science.gov (United States)

    Schalbetter, Stephanie A; Mansoubi, Sahar; Chambers, Anna L; Downs, Jessica A; Baxter, Jonathan

    2015-08-18

    Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA precatenation formed. Although fork rotation predominates at replication termination, in vitro studies have suggested that it also occurs frequently during elongation. However, the factors that influence fork rotation and how rotation and precatenation may influence other replication-associated processes are unknown. Here we analyze the causes and consequences of fork rotation in budding yeast. We find that fork rotation and precatenation preferentially occur in contexts that inhibit topoisomerase action ahead of the fork, including stable protein-DNA fragile sites and termination. However, generally, fork rotation and precatenation are actively inhibited by Timeless/Tof1 and Tipin/Csm3. In the absence of Tof1/Timeless, excessive fork rotation and precatenation cause extensive DNA damage following DNA replication. With Tof1, damage related to precatenation is focused on the fragile protein-DNA sites where fork rotation is induced. We conclude that although fork rotation and precatenation facilitate unwinding in hard-to-replicate contexts, they intrinsically disrupt normal chromosome duplication and are therefore restricted by Timeless/Tipin.

  6. RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.

    Science.gov (United States)

    Elia, Andrew E H; Wang, David C; Willis, Nicholas A; Boardman, Alexander P; Hajdu, Ildiko; Adeyemi, Richard O; Lowry, Elizabeth; Gygi, Steven P; Scully, Ralph; Elledge, Stephen J

    2015-10-15

    We have used quantitative proteomics to profile ubiquitination in the DNA damage response (DDR). We demonstrate that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling. Ubiquitination of RPA occurs on chromatin, involves sites outside its DNA binding channel, does not cause proteasomal degradation, and increases under conditions of fork collapse, suggesting a role in repair at stalled forks. We demonstrate that the E3 ligase RFWD3 mediates RPA ubiquitination. RFWD3 is necessary for replication fork restart, normal repair kinetics during replication stress, and homologous recombination (HR) at stalled replication forks. Mutational analysis suggests that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Multisite protein group sumoylation is known to promote HR in yeast. Our findings reveal a similar requirement for multisite protein group ubiquitination during HR at stalled forks in mammalian cells. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. The fecundity of fork-tailed threadfin bream (Nemipterus furcosus) in Bangka, Bangka Belitung

    Science.gov (United States)

    Utami, E.; Safitriyani, E.; Gatra Persada, Leo

    2018-04-01

    Fork-tailed threadfin bream (Nemipterus furcosus) is one of important economic fishes in Bangka. The sustainability of fork-tailed threadfin bream is threatened by degradation of natural habitat. Information of reproductive is needed for further management. The objective of this study was to examine fecundity of fork-tailed threadfin bream. The mean values of temperature was 28.83 ± 0,37°C, respectively. Sex ratio during sampling showed that female fork-tailed threadfin bream greater than male population. Berried female fork-tailed threadfin bream found from March until November. The greatest number of berried female fork-tailed threadfin bream showed in July with berried female value of 25. Fork-tailed threadfin bream fecundity was 19951 and 66628, respectively. The fecundity data can be used to access the reproductive potential of fish stock and also as an assessment on stock size of their natural population.

  8. Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication.

    Science.gov (United States)

    Morrow, Carl A; Nguyen, Michael O; Fower, Andrew; Wong, Io Nam; Osman, Fekret; Bryer, Claire; Whitby, Matthew C

    2017-06-06

    Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. Collapsed forks can be rescued by homologous recombination, which restarts replication. However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. Based on our findings, we propose that IFSA is a potential threat to genomic stability in eukaryotes.

  9. Termination of DNA replication forks: "Breaking up is hard to do".

    Science.gov (United States)

    Bailey, Rachael; Priego Moreno, Sara; Gambus, Agnieszka

    2015-01-01

    To ensure duplication of the entire genome, eukaryotic DNA replication initiates from thousands of replication origins. The replication forks move through the chromatin until they encounter forks from neighboring origins. During replication fork termination forks converge, the replisomes disassemble and topoisomerase II resolves the daughter DNA molecules. If not resolved efficiently, terminating forks result in genomic instability through the formation of pathogenic structures. Our recent findings shed light onto the mechanism of replisome disassembly upon replication fork termination. We have shown that termination-specific polyubiquitylation of the replicative helicase component - Mcm7, leads to dissolution of the active helicase in a process dependent on the p97/VCP/Cdc48 segregase. The inhibition of terminating helicase disassembly resulted in a replication termination defect. In this extended view we present hypothetical models of replication fork termination and discuss remaining and emerging questions in the DNA replication termination field.

  10. Channelization and floodplain forests: impacts of accelerated sedimentation and valley plug formation on floodplain forests of the Middle Fork Forked Deer River, Tennessee, USA

    Science.gov (United States)

    Sonja N. Oswalt; Sammy L. King

    2005-01-01

    We evaluated the severe degradation of floodplain habitats resulting from channelization and concomitant excessive coarse sedimentation on the Middle Fork Forked Deer River in west Tennessee from 2000 to 2003. Land use practices have resulted in excessive sediment in the tributaries and river system eventually resulting in sand deposition on the floodplain, increased...

  11. Replication fork stability confers chemoresistance in BRCA-deficient cells

    DEFF Research Database (Denmark)

    Chaudhuri, Arnab Ray; Callen, Elsa; Ding, Xia

    2016-01-01

    /4 complex protein, PTIP, protects Brca1/2-deficient cells from DNA damage and rescues the lethality of Brca2-deficient embryonic stem cells. However, PTIP deficiency does not restore homologous recombination activity at double-strand breaks. Instead, its absence inhibits the recruitment of the MRE11......Cells deficient in the Brca1 and Brca2 genes have reduced capacity to repair DNA double-strand breaks by homologous recombination and consequently are hypersensitive to DNA-damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3...... nuclease to stalled replication forks, which in turn protects nascent DNA strands from extensive degradation. More generally, acquisition of PARP inhibitors and cisplatin resistance is associated with replication fork protection in Brca2-deficient tumour cells that do not develop Brca2 reversion mutations...

  12. A vibrating quartz fork - a tool for cryogenic helium research

    Czech Academy of Sciences Publication Activity Database

    Blažková, Michaela; Člověčko, M.; Eltsov, V. B.; Gažo, E.; de Graaf, R.; Hosio, J.J.; Krusius, M.; Schmoranzer, D.; Schoepe, W.; Skrbek, Ladislav; Skyba, P.; Solntsev, R.E.; Vinen, W. F.

    2008-01-01

    Roč. 150, - (2008), s. 525-535 ISSN 0022-2291 R&D Projects: GA ČR GA202/05/0218 Grant - others:GAUK(CZ) 7953/2007; Transnational Access Programme(XE) RITA -CT-2003-505313 Institutional research plan: CEZ:AV0Z10100520 Keywords : normal 3He * superfluid 3He * superfluid 4He * turbulence, * cavitation * quartz tuning fork Subject RIV: BK - Fluid Dynamics Impact factor: 1.034, year: 2008

  13. Pitch Fork: A Novel tactile Digital Musical Instrument

    OpenAIRE

    Williams, Peter; Overholt, Daniel

    2017-01-01

    Pitch Fork is a prototype of an alternate, actuated digital musical instrument (DMI). It uses 5 infra-red and 4 piezoelectric sensors to control an additive synthesis engine. Iron bars are used as the physical point of contact in interaction with the aim of using material computation to control aspects of the digitally produced sound. This choice of material was also chosen to affect player experience. Sensor readings are relayed to a Macbook via an Arduino Mega. Mappings and audio output sig...

  14. Timing, coordination, and rhythm: Acrobatics at the DNA replication fork

    KAUST Repository

    Hamdan, Samir

    2010-04-09

    In DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

  15. Cell lethality after selective irradiation of the DNA replication fork

    International Nuclear Information System (INIS)

    Hofer, K.G.; Warters, R.L.

    1985-01-01

    It has been suggested that nascent DNA located at the DNA replication fork may exhibit enhanced sensitivity to radiation damage. To evaluate this hypothesis, Chinese hamster ovary cells (CHO) were labeled with 125 I-iododeoxyuridine ( 125 IUdR) either in the presence or absence of aphidicolin. Aphidicolin (5 μg/ml) reduced cellular 125 IUdR incorporation to 3-5% of the control value. The residual 125 I incorporation appeared to be restricted to low molecular weight (sub-replicon sized) fragments of DNA which were more sensitive to micrococcal nuclease attack and less sensitive to high salt DNase I digestion than randomly labeled DNA. These findings suggest that DNA replicated in the presence of aphidicolin remains localized at the replication fork adjacent to the nuclear matrix. Based on these observations an attempt was made to compare the lethal consequences of 125 I decays at the replication fork to that of 125 I decays randomly distributed over the entire genome. Regardless of the distribution of decay events, all treatment groups exhibited identical dose-response curves (D 0 : 101 125 I decays/cell). Since differential irradiation of the replication complex did not result in enhanced cell lethality, it can be concluded that neither the nascent DNA nor the protein components (replicative enzymes, nuclear protein matrix) associated with the DNA replication site constitute key radiosensitive targets within the cellular genome. (orig.)

  16. Timing, coordination, and rhythm: Acrobatics at the DNA replication fork

    KAUST Repository

    Hamdan, Samir; van Oijen, Antoine M.

    2010-01-01

    In DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Recovery of arrested replication forks by homologous recombination is error-prone.

    Directory of Open Access Journals (Sweden)

    Ismail Iraqui

    Full Text Available Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability. Here, we report that recovery of impeded replication forks by homologous recombination is error-prone. Using a fork-arrest-based assay in fission yeast, we demonstrate that a single collapsed fork can cause mutations and large-scale genomic changes, including deletions and translocations. Fork-arrest-induced gross chromosomal rearrangements are mediated by inappropriate ectopic recombination events at the site of collapsed forks. Inverted repeats near the site of fork collapse stimulate large-scale genomic changes up to 1,500 times over spontaneous events. We also show that the high accuracy of DNA replication during S-phase is impaired by impediments to fork progression, since fork-arrest-induced mutation is due to erroneous DNA synthesis during recovery of replication forks. The mutations caused are small insertions/duplications between short tandem repeats (micro-homology indicative of replication slippage. Our data establish that collapsed forks, but not stalled forks, recovered by homologous recombination are prone to replication slippage. The inaccuracy of DNA synthesis does not rely on PCNA ubiquitination or trans-lesion-synthesis DNA polymerases, and it is not counteracted by mismatch repair. We propose that deletions/insertions, mediated by micro-homology, leading to copy number variations during replication stress may arise by progression of error-prone replication forks restarted by homologous recombination.

  18. Archaeological Investigations on the East Fork of the Salmon River, Custer County, Idaho.

    Science.gov (United States)

    1984-01-01

    coniferous environment in addition to pine marten (Martes americana), red squirrel (Tamiasciurus hudsonicus), porcupine (Erithizon dorsatum), mountain vole...can be seen in small herds throughout the East Fork valley from the Salmon River to Big Boulder Creek. Two bands of Rocky Mountain bighorn sheep...utilize the Challis Planning Unit, one on the East Fork and the other in the Birch Creek area. The East Fork herd is comprised of approximately 50-70

  19. Oriented Onion Sowing by a Forked-Roller Sowing Unit

    Directory of Open Access Journals (Sweden)

    Aleksandr G.

    2018-03-01

    Full Text Available Introduction: The existing sowing machines do not provide a single feeding of the bulbs with a planting (sowing unit that leads to a violation of the agrotechnical requirements of planting bulbs. It is necessary to search new solutions to preserve the position of the bulbs in the furrow with the bottom down and their regularly spaced distribution. Materials and Methods: The article presents the design for a prototype for a planting machine equipped with a forked-roller sowing unit for orienting the onion-sowing into a furrow. Testing the forked-roller sowing unit were carried out on a flat area where the physical and mechanical properties of the soil were determined on the days of sowing, and the indices of the quality of the onion-sowing were determined. The study of the effect of the sowing machine speed on the quality of the onion-seed bulb landing was determined by the change in the translational speed of the sowing unit in the range of 0.8 m/s to 1.2 m/s with a variation interval of 0.1 m/s. The indicators of the quality of the planting of the bulbs were determined by the opening of the closed furrow. The results of laboratory-field studies of the planting machine prototype are presented. Results: The results of laboratory-field studies of a planting machine equipped with a forked-roller sowing unit for planting onion bulbs are presented. The optimal technological parameters are determined experimentally. It was determined the number of bulbs that are for up is 51 % and the regularity of planting by the forked-roller sowing unit – 79 %. These figures are provided at the forward speed of the planting machine VM = 0.9–1.0 m/s, the height of the fall of the bulb HA = 0.12 m, and the rotation frequency of the landing drum nБ = 0.47 c-1. Discussion and Conclusions: The use of a forked-roller sowing unit makes it possible to increase the proportion of onions planted by bottom down by 200 %, and the uniformity of planting bulbs by 19 %, in

  20. Take It Slow: can feedback from a smart fork reduce eating speed?

    Directory of Open Access Journals (Sweden)

    Sander Hermsen

    2015-09-01

    The present study examines the efficacy of a smart fork that helps people to eat more slowly. This adapted fork records eating speed and delivers vibrotactile feedback if users eat too quickly. In two studies, we tested the acceptability and user experience of the fork (Study 1, and its effect on eating rate and satiety levels in a controlled lab-setting (Study 2. Method: In study 1, 11 participants (all self-reported fast eaters ate a meal using the fork in our laboratory and used the fork for three consecutive days in their home setting. Participants took part in semi-structured interviews after the first meal and upon returning the fork, covering perceived effect on eating rate, comfort of use, accuracy, and motivational and social aspects of fork use. Interviews were coded and a thematic classification analysis was performed. In study 2, 128 participants (all self-reported fast eaters ate a standardized meal using the fork in our laboratory. We used a between-participants design with 2 conditions; participants ate their meal either with vibrotactile feedback from the fork (experimental condition or ate their meal without vibrotactile feedback (control condition. Eating rate, meal duration, error rate (number of bites taken faster than 10 seconds after previous bite, total food intake, and satiety were recorded for every participant. Results: Study 1: All participants felt that the feedback was generally accurate and consistent. Fork size, weight, and intensity of the feedback were seen as comfortable and acceptable. All participants reported a heightened awareness of eating rate and all but one participant reported eating more slowly with the fork. Study 2: Participants in the experimental condition ate significantly slower, and with a lower error rate than those in the control condition. Feedback did not significantly affect the amount of food eaten. Conclusions: Our findings suggest that this smart fork is an acceptable and effective tool to disrupt and

  1. The fork and the kinase: a DNA replication tale from a CHK1 perspective.

    Science.gov (United States)

    González Besteiro, Marina A; Gottifredi, Vanesa

    2015-01-01

    Replication fork progression is being continuously hampered by exogenously introduced and naturally occurring DNA lesions and other physical obstacles. Checkpoint kinase 1 (Chk1) is activated at replication forks that encounter damaged DNA. Subsequently, Chk1 inhibits the initiation of new replication factories and stimulates the firing of dormant origins (those in the vicinity of stalled forks). Chk1 also avoids fork collapse into DSBs (double strand breaks) and promotes fork elongation. At the molecular level, the current model considers stalled forks as the site of Chk1 activation and the nucleoplasm as the location where Chk1 phosphorylates target proteins. This model certainly serves to explain how Chk1 modulates origin firing, but how Chk1 controls the fate of stalled forks is less clear. Interestingly, recent reports demonstrating that Chk1 phosphorylates chromatin-bound proteins and even holds kinase-independent functions might shed light on how Chk1 contributes to the elongation of damaged DNA. Indeed, such findings have unveiled a puzzling connection between Chk1 and DNA lesion bypass, which might be central to promoting fork elongation and checkpoint attenuation. In summary, Chk1 is a multifaceted and versatile signaling factor that acts at ongoing forks and replication origins to determine the extent and quality of the cellular response to replication stress. Copyright © 2014 Elsevier B.V. All rights reserved.

  2. Implementing farm-to-fork traceability in Tanzania

    CSIR Research Space (South Africa)

    Van Dyk, FE

    2005-08-01

    Full Text Available stream_source_info Van Dyk2_2005.pdf.txt stream_content_type text/plain stream_size 10949 Content-Encoding UTF-8 stream_name Van Dyk2_2005.pdf.txt Content-Type text/plain; charset=UTF-8 Copyright @ CSIR 2005 www....csir.co.za Implementing farm-to-fork traceability in Tanzania Esbeth van Dyk CSIR Centre for Logistics ORSSA/SAIIE August 2005 Copyright @ CSIR 2005 www.csir.co.za Structure • Why traceability? • Legislation • Tanzania project • Recordkeeping in coffee...

  3. Burnup measurements with the Los Alamos fork detector

    International Nuclear Information System (INIS)

    Bosler, G.E.; Rinard, P.M.

    1991-01-01

    The fork detector system can determine the burnup of spent-fuel assemblies. It is a transportable instrument that can be mounted permanently in a spent-fuel pond near a loading area for shipping casks, or be attached to the storage pond bridge for measurements on partially raised spent-fuel assemblies. The accuracy of the predicted burnup has been demonstrated to be as good as 2% from measurements on assemblies in the United States and other countries. Instruments have also been developed at other facilities throughout the world using the same or different techniques, but with similar accuracies. 14 refs., 2 figs., 2 tabs

  4. Multiplexing of adjacent vortex modes with the forked grating coupler

    Science.gov (United States)

    Nadovich, Christopher T.; Kosciolek, Derek J.; Crouse, David T.; Jemison, William D.

    2017-08-01

    For vortex fiber multiplexing to reach practical commercial viability, simple silicon photonic interfaces with vortex fiber will be required. These interfaces must support multiplexing. Toward this goal, an efficient singlefed multimode Forked Grating Coupler (FGC) for coupling two different optical vortex OAM charges to or from the TE0 and TE1 rectangular waveguide modes has been developed. A simple, apodized device implemented with e-beam lithography and a conventional dual-etch processing on SOI wafer exhibits low crosstalk and reasonable mode match. Advanced designs using this concept are expected to further improve performance.

  5. Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks

    DEFF Research Database (Denmark)

    Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia

    2016-01-01

    Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose...... RAD52 facilitates repair of collapsed DNA replication forks in cancer cells....

  6. 76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

    Science.gov (United States)

    2011-06-20

    ... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

  7. 76 FR 46721 - Salmon-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project...

    Science.gov (United States)

    2011-08-03

    ...-Challis National Forest, ID; Upper North Fork HFRA Ecosystem Restoration Project Environmental Impact... improve the health of the ecosystem and reach the desired future condition. DATES: Comments concerning the... Ecosystem Restoration Project EIS, P.O. Box 180, 11 Casey Rd., North Fork, ID 83466. Comments may also be...

  8. Both DNA Polymerases δ and ε Contact Active and Stalled Replication Forks Differently

    Science.gov (United States)

    Yu, Chuanhe; Gan, Haiyun

    2017-01-01

    ABSTRACT Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase has a distinct pattern of association with active and stalled replication forks. Pol α is enriched at extending Okazaki fragments of active and stalled forks. In contrast, although Pol δ contacts the nascent lagging strands of active and stalled forks, it binds to only the matured (and not elongating) Okazaki fragments of stalled forks. Pol ε has greater contact with the nascent single-stranded DNA (ssDNA) of the leading strand on active forks than on stalled forks. We propose that the configuration of DNA polymerases at stalled forks facilitates the resumption of DNA synthesis after stress removal. PMID:28784720

  9. A watershed's response to logging and roads: South Fork of Caspar Creek, California, 1967-1976

    Science.gov (United States)

    Raymond M. Rice; Forest B. Tilley; Patricia A. Datzman

    1979-01-01

    The effect of logging and roadbuilding on erosion and sedimentation are analyzed by comparing the North Fork and South Fork of Caspar Creek, in northern California. Increased sediment production during the 4 years after road construction, was 326 cu yd/sq mi/yr—80 percent greater than that predicted by the predisturbance regression analysis. The average...

  10. Improve performance of scanning probe microscopy by balancing tuning fork prongs

    International Nuclear Information System (INIS)

    Ng, Boon Ping; Zhang Ying; Wei Kok, Shaw; Chai Soh, Yeng

    2009-01-01

    This paper presents an approach for improving the Q-factor of tuning fork probe used in scanning probe microscopes. The improvement is achieved by balancing the fork prongs with extra mass attachment. An analytical model is proposed to characterize the Q-factor of a tuning fork probe with respect to the attachment of extra mass on the tuning fork prongs, and based on the model, the Q-factors of the unbalanced and balanced tuning fork probes are derived and compared. Experimental results showed that the model fits well the experimental data and the approach can improve the Q-factor by more than a factor of three. The effectiveness of the approach is further demonstrated by applying the balanced probe on an atomic force microscope to obtain improved topographic images.

  11. FANCJ couples replication past natural fork barriers with maintenance of chromatin structure.

    Science.gov (United States)

    Schwab, Rebekka A; Nieminuszczy, Jadwiga; Shin-ya, Kazuo; Niedzwiedz, Wojciech

    2013-04-01

    Defective DNA repair causes Fanconi anemia (FA), a rare childhood cancer-predisposing syndrome. At least 15 genes are known to be mutated in FA; however, their role in DNA repair remains unclear. Here, we show that the FANCJ helicase promotes DNA replication in trans by counteracting fork stalling on replication barriers, such as G4 quadruplex structures. Accordingly, stabilization of G4 quadruplexes in ΔFANCJ cells restricts fork movements, uncouples leading- and lagging-strand synthesis and generates small single-stranded DNA gaps behind the fork. Unexpectedly, we also discovered that FANCJ suppresses heterochromatin spreading by coupling fork movement through replication barriers with maintenance of chromatin structure. We propose that FANCJ plays an essential role in counteracting chromatin compaction associated with unscheduled replication fork stalling and restart, and suppresses tumorigenesis, at least partially, in this replication-specific manner.

  12. The analyst's body as tuning fork: embodied resonance in countertransference.

    Science.gov (United States)

    Stone, Martin

    2006-02-01

    This paper examines the phenomenon of embodied countertransference: where the analyst experiences a somatic reaction rather than the more common countertransference responses of thoughts, feelings, images, fantasies and dreams. Discussion of clinical material considers neurotic and syntonic aspects. The analogy is made of resonance with a tuning fork. Several questions are posed: Why does countertransference resonate in the bodies of some analysts but not all? Why do those analysts who are sensitive to this, experience it with some patients but not with others? And what are the conditions which are conducive to producing somatic responses? It proposes that somatic reactions are more likely to occur when a number of conditions come together: when working with patients exhibiting borderline, psychotic or severe narcissistic elements; where there has been early severe childhood trauma; and where there is fear of expressing strong emotions directly. In addition another theoretical factor is proposed, namely the typology of the analyst.

  13. Theoretical model and optimization of a novel temperature sensor based on quartz tuning fork resonators

    International Nuclear Information System (INIS)

    Xu Jun; You Bo; Li Xin; Cui Juan

    2007-01-01

    To accurately measure temperatures, a novel temperature sensor based on a quartz tuning fork resonator has been designed. The principle of the quartz tuning fork temperature sensor is that the resonant frequency of the quartz resonator changes with the variation in temperature. This type of tuning fork resonator has been designed with a new doubly rotated cut work at flexural vibration mode as temperature sensor. The characteristics of the temperature sensor were evaluated and the results sufficiently met the target of development for temperature sensor. The theoretical model for temperature sensing has been developed and built. The sensor structure was analysed by finite element method (FEM) and optimized, including tuning fork geometry, tine electrode pattern and the sensor's elements size. The performance curve of output versus measured temperature is given. The results from theoretical analysis and experiments indicate that the sensor's sensitivity can reach 60 ppm 0 C -1 with the measured temperature range varying from 0 to 100 0 C

  14. The Sound Field around a Tuning Fork and the Role of a Resonance Box

    Science.gov (United States)

    Bogacz, Bogdan F.; Pedziwiatr, Antoni T.

    2015-01-01

    Atypical two-tine tuning fork is barely audible when held vibrating at an arm's length. It is enough, however, to touch its base to a table or, better, to a resonance box and the emitted sound becomes much louder. An inquiring student may pose questions: (1) Why is a bare tuning fork such a weak emitter of sound? (2) What is the role of the…

  15. Checkpoint-dependent RNR induction promotes fork restart after replicative stress.

    Science.gov (United States)

    Morafraile, Esther C; Diffley, John F X; Tercero, José Antonio; Segurado, Mónica

    2015-01-20

    The checkpoint kinase Rad53 is crucial to regulate DNA replication in the presence of replicative stress. Under conditions that interfere with the progression of replication forks, Rad53 prevents Exo1-dependent fork degradation. However, although EXO1 deletion avoids fork degradation in rad53 mutants, it does not suppress their sensitivity to the ribonucleotide reductase (RNR) inhibitor hydroxyurea (HU). In this case, the inability to restart stalled forks is likely to account for the lethality of rad53 mutant cells after replication blocks. Here we show that Rad53 regulates replication restart through the checkpoint-dependent transcriptional response, and more specifically, through RNR induction. Thus, in addition to preventing fork degradation, Rad53 prevents cell death in the presence of HU by regulating RNR-expression and localization. When RNR is induced in the absence of Exo1 and RNR negative regulators, cell viability of rad53 mutants treated with HU is increased and the ability of replication forks to restart after replicative stress is restored.

  16. Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.

    Science.gov (United States)

    Reynolds, John J; Bicknell, Louise S; Carroll, Paula; Higgs, Martin R; Shaheen, Ranad; Murray, Jennie E; Papadopoulos, Dimitrios K; Leitch, Andrea; Murina, Olga; Tarnauskaitė, Žygimantė; Wessel, Sarah R; Zlatanou, Anastasia; Vernet, Audrey; von Kriegsheim, Alex; Mottram, Rachel M A; Logan, Clare V; Bye, Hannah; Li, Yun; Brean, Alexander; Maddirevula, Sateesh; Challis, Rachel C; Skouloudaki, Kassiani; Almoisheer, Agaadir; Alsaif, Hessa S; Amar, Ariella; Prescott, Natalie J; Bober, Michael B; Duker, Angela; Faqeih, Eissa; Seidahmed, Mohammed Zain; Al Tala, Saeed; Alswaid, Abdulrahman; Ahmed, Saleem; Al-Aama, Jumana Yousuf; Altmüller, Janine; Al Balwi, Mohammed; Brady, Angela F; Chessa, Luciana; Cox, Helen; Fischetto, Rita; Heller, Raoul; Henderson, Bertram D; Hobson, Emma; Nürnberg, Peter; Percin, E Ferda; Peron, Angela; Spaccini, Luigina; Quigley, Alan J; Thakur, Seema; Wise, Carol A; Yoon, Grace; Alnemer, Maha; Tomancak, Pavel; Yigit, Gökhan; Taylor, A Malcolm R; Reijns, Martin A M; Simpson, Michael A; Cortez, David; Alkuraya, Fowzan S; Mathew, Christopher G; Jackson, Andrew P; Stewart, Grant S

    2017-04-01

    To ensure efficient genome duplication, cells have evolved numerous factors that promote unperturbed DNA replication and protect, repair and restart damaged forks. Here we identify downstream neighbor of SON (DONSON) as a novel fork protection factor and report biallelic DONSON mutations in 29 individuals with microcephalic dwarfism. We demonstrate that DONSON is a replisome component that stabilizes forks during genome replication. Loss of DONSON leads to severe replication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks. Furthermore, ATM- and Rad3-related (ATR)-dependent signaling in response to replication stress is impaired in DONSON-deficient cells, resulting in decreased checkpoint activity and the potentiation of chromosomal instability. Hypomorphic mutations in DONSON substantially reduce DONSON protein levels and impair fork stability in cells from patients, consistent with defective DNA replication underlying the disease phenotype. In summary, we have identified mutations in DONSON as a common cause of microcephalic dwarfism and established DONSON as a critical replication fork protein required for mammalian DNA replication and genome stability.

  17. Age

    Science.gov (United States)

    ... adults? How can you reduce anesthesia risks in older patients? Age Age may bring wisdom but it also brings ... Ask your physician to conduct a pre-surgery cognitive test — an assessment of your mental function. The physician can use the results as a ...

  18. Ultrasonic superlensing jets and acoustic-fork sheets

    Energy Technology Data Exchange (ETDEWEB)

    Mitri, F.G., E-mail: F.G.Mitri@ieee.org

    2017-05-18

    Focusing acoustical (and optical) beams beyond the diffraction limit has remained a major challenge in imaging instruments and systems, until recent advances on “hyper” or “super” lensing and higher-resolution imaging techniques have shown the counterintuitive violation of this rule under certain circumstances. Nonetheless, the proposed technologies of super-resolution acoustical focusing beyond the diffraction barrier require complex tools such as artificially engineered metamaterials, and other hardware equipment that may not be easily synthesized or manufactured. The present contribution therefore suggests a simple and reliable method of using a sound-penetrable circular cylinder lens illuminated by a nonparaxial Gaussian acoustical sheet (i.e. finite beam in 2D) to produce non-evanescent ultrasonic superlensing jets (or bullets) and acoustical ‘snail-fork’ shaped wavefronts with limited diffraction. The generalized (near-field) scattering theory for acoustical sheets of arbitrary wavefronts and incidence is utilized to synthesize the incident beam based upon the angular spectrum decomposition method and the multipole expansion method in cylindrical wave functions to compute the scattered pressure around the cylinder with particular emphasis on its physical properties. The results show that depending on the beam and lens parameters, a tight focusing (with dimensions much smaller than the beam waist) can be achieved. Subwavelength resolution can be also achieved by selecting a lens material with a speed of sound exceeding that of the host fluid medium. The ultrasonic superlensing jets provide the impetus to develop improved subwavelength microscopy and acoustical image-slicing systems, cell lysis and surgery, and photoacoustic imaging to name a few examples. Moreover, an acoustical fork-sheet generation may open innovative avenues in reconfigurable on-chip micro/nanoparticle tweezers and surface acoustic waves devices. - Highlights: • Ultrasonic

  19. Note: Enhanced energy harvesting from low-frequency magnetic fields utilizing magneto-mechano-electric composite tuning-fork.

    Science.gov (United States)

    Yang, Aichao; Li, Ping; Wen, Yumei; Yang, Chao; Wang, Decai; Zhang, Feng; Zhang, Jiajia

    2015-06-01

    A magnetic-field energy harvester using a low-frequency magneto-mechano-electric (MME) composite tuning-fork is proposed. This MME composite tuning-fork consists of a copper tuning fork with piezoelectric Pb(Zr(1-x)Ti(x))O3 (PZT) plates bonded near its fixed end and with NdFeB magnets attached at its free ends. Due to the resonance coupling between fork prongs, the MME composite tuning-fork owns strong vibration and high Q value. Experimental results show that the proposed magnetic-field energy harvester using the MME composite tuning-fork exhibits approximately 4 times larger maximum output voltage and 7.2 times higher maximum power than the conventional magnetic-field energy harvester using the MME composite cantilever.

  20. Aging

    International Nuclear Information System (INIS)

    Sasaki, Hideo; Kodama, Kazunori; Yamada, Michiko

    1991-01-01

    The hypothesis that exposure to ionizing radiation accelerates the aging process has been actively investigated at ABCC-RERF since 1958, when longitudinal cohort studies of the Adult Health Study (AHS) and the Life Span Study (LSS) were initiated. In their 1975 overall review of aging studies related to the atomic bomb (A-bomb) survivors, Finch and Beebe concluded that while most studies had shown no correlation between aging and radiation exposure, they had not involved the large numbers of subjects required to provide strong evidence for or against the hypothesis. Extending LSS mortality data up to 1978 did not alter the earlier conclusion that any observed life-shortening was associated primarily with cancer induction rather than with any nonspecific cause. The results of aging studies conducted during the intervening 15 years using data from the same populations are reviewed in the present paper. Using clinical, epidemiological, and laboratory techniques, a broad spectrum of aging parameters have been studied, such as postmortem morphological changes, tests of functional capacity, physical tests and measurements, laboratory tests, tissue changes, and morbidity. With respect to the aging process, the overall results have not been consistent and are generally thought to show no relation to radiation exposure. Although some preliminary results suggest a possible radiation-induced increase in atherosclerotic diseases and acceleration of aging in the T-cell-related immune system, further study is necessary to confirm these findings. In the future, applying the latest gerontological study techniques to data collected from subjects exposed 45 years ago to A-bomb radiation at relatively young ages will present a new body of data relevant to the study of late radiation effects. (author) 103 refs

  1. Aging

    International Nuclear Information System (INIS)

    Finch, S.C.; Beebe, G.W.

    1975-01-01

    The hypothesis that ionizing radiation accelerates natural aging has been under investigation at the Atomic Bomb Casualty Commission since 1959. Postmortem observations of morphologic and chemical changes, tests of functional capacity, physical tests and measurements, clinical laboratory tests, tissue changes, morbidity, and mortality have all been examined by ABCC investigators interested in this hypothesis. These studies have been beset with conceptual difficulties centered on the definition and measurement of aging. An empirical approach early led to the calculation of an index of physiologic age as a linear combination of age-related tests of various organ systems. Most studies have been negative but have not involved the large numbers that might be required to provide strong evidence for or against the hypothesis. Mortality, however, has been examined on the basis of a large sample and over the period 1950-1972 had provided no support for the hypothesis of radiation-accelerated aging. Ionizing radiation dose, of course shorten human life, but its life-shortening effect appears to be the result of specific radiation-induced disease, especially neoplasms. The hypothesis is now much less attractive than it was 10-20 years ago but still has some value in stimulating research on aging. The experience of the A-bomb survivors provides an unusual opportunity for a definitive test of the hypothesis. (auth.)

  2. Soil Investigation of Lower East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Dickson, Johnbull O [ORNL; Mayes, Melanie [ORNL; Earles, Jennifer E [ORNL; Mehlhorn, Tonia L [ORNL; Lowe, Kenneth Alan [ORNL; Peterson, Mark J [ORNL; Pierce, Eric M [ORNL

    2017-03-01

    Mercury is regarded by the US Department of Energy (DOE) Oak Ridge Office of Environmental Management as a priority contaminant on the Oak Ridge Reservation because of the environmental risks associated with substantial losses from buildings, soils, and surface waters at the Y-12 National Security Complex (Y-12). As a result of historical releases of mercury from Y-12 primarily in the 1950s and early 1960s, the lower East Fork Poplar Creek (LEFPC) stream channel and bank soil margins are contaminated with mercury (Brooks and Southworth 2011; Tennessee Valley Authority 1985b, a). A Mercury Remediation Technology Development project is underway to evaluate the nature of downstream mercury contamination and to develop targeted site-specific remedial technologies that can mitigate mercury release and biological uptake. It is known that mercury concentration varies longitudinally and with depth in LEFPC bank soils; however, soil types and soil physical properties are not well known, especially relative to the zones of mercury contamination. Moreover, there are no soil maps for the downstream reaches of LEFPC in Roane County (i.e. from the Chestnut Hill Road downstream) and this work represents the first ever soil mapping along this section of LEFPC.

  3. BIOLOGICAL MONITORING PROGRAM FOR EAST FORK POPLAR CREEK

    Energy Technology Data Exchange (ETDEWEB)

    ADAMS, S.M.; ASHWOOD, T.L.; BEATY, T.W.; BRANDT, C.C.

    1997-10-24

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y- 12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  4. Biological monitoring program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S.M.; Ashwood, T.L.; Beaty, T.W.; Brandt, C.C.; Christensen, S.W.; Cicerone, D.S.; Greeley, M.S. Jr.; Hill, W.R.; Kszos, L.S.

    1997-04-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Lear et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities.

  5. Tuning Fork Oscillators as Downhole Viscometers in Oilfield Applications

    Science.gov (United States)

    Gonzalez, Miguel; Bernero, Greg; Alvarez, Oliverio; Ham, Gregory; Max, Deffenbaugh; Sensors Development Team

    2015-03-01

    The commerciality of oil wells is greatly influenced by the physical properties of the fluids being produced. A key parameter in determining how producible the hydrocarbons are is their viscosity. Pressure and temperature changes in recovering a downhole sample to the surface can alter viscosity while accurate downhole measurement of this critical property remains a rudimentary effort in the industry. In this presentation we describe the challenges of measuring and quantifying the viscosity of reservoir fluids in situ at downhole conditions, as well as present an overview of some of the different measurement techniques currently used. Additionally, we show our characterization of a piezoelectric tuning fork oscillator used as a viscosity sensor. In an attempt to recreate the environment found in oil wells, its mechanical and electrical properties were studied while the device was immersed in different fluids and, separately, under different conditions of pressure and temperature. This device is a first step toward the development of an inexpensive, integrated, and miniaturized sensing platform for the in situ characterization of reservoir fluids.

  6. Bioavailability of mercury in East Fork Poplar Creek soils

    International Nuclear Information System (INIS)

    Barnett, M.O.; Turner, R.R.

    1995-05-01

    The initial risk assessment for the East Fork Poplar Creek (EFPC) floodplain in Oak Ridge, Tennessee, a superfund site heavily contaminated with mercury, was based upon a reference dose for mercuric chloride, a soluble mercury compound not expected to be present in the floodplain, which is frequently saturated with water. Previous investigations had suggested mercury in the EFPC floodplain was less soluble and therefore less bioavailable than mercuric chloride, possibly making the results of the risk assessment unduly conservative. A bioavailability study, designed to measure the amount of mercury available for absorption in a child's digestive tract, the most critical risk endpoint and pathway, was performed on twenty soils from the EFPC floodplain. The average percentage of mercury released during the study for the twenty soils was 5.3%, compared to 100% of the compound mercuric chloride subjected to the same conditions. Alteration of the procedure to test additional conditions possible during soil digestion did not appreciably alter the results. Therefore, use of a reference dose for mercuric chloride in the EFPC risk assessment without inclusion of a corresponding bioavailability factor may be unduly conservative

  7. Failure analysis of axle shaft of a fork lift

    Directory of Open Access Journals (Sweden)

    Souvik Das

    2015-04-01

    Full Text Available An axle shaft of fork lift failed at operation within 296 h of service. The shaft transmits torque from discrepancy to wheel through planetary gear arrangement. A section of fractured axle shaft made of induction-hardened steel was analyzed to determine the root cause of the failure. Optical microscopies as well as field emission gun scanning electron microscopy (FEG-SEM along with energy dispersive spectroscopy (EDS were carried out to characterize the microstructure. Hardness profile throughout the cross-section was evaluated by micro-hardness measurements. Chemical analysis indicated that the shaft was made of 42CrMo4 steel grade as per specification. Microstructural analysis and micro-hardness profile revealed that the shaft was improperly heat treated resulting in a brittle case, where crack was found to initiate from the case in a brittle mode in contrast to ductile mode within the core. This behaviour was related to differences in microstructure, which was observed to be martensitic within the case with a micro-hardness equivalent to 735 HV, and a mixture of non-homogeneous structure of pearlite and ferrite within the core with a hardness of 210 HV. The analysis suggests that the fracture initiated from the martensitic case as brittle mode due to improper heat treatment process (high hardness. Moreover the inclusions along the hot working direction i.e. in the longitudinal axis made the component more susceptible to failure.

  8. Final Environmental Assessment: Installation of Digital Airport Surveillance Radar at Grand Forks Air Force Base, North Dakota

    Science.gov (United States)

    2011-05-11

    Grand Forks AFB public web site. Notices of Availability were published in the Grand Forks Herald on 10 Mar 2011 and on the Grand Forks AFB web site from...Squadron (319th) FTA Fire Training Area GATR Ground-Air Transmit Receive GFAFB Grand Forks Air Force Base GHG Greenhouse Gas Hz Hertz IEEE Institute of...feet west of the closed/capped ERP Site FT-02, the Fire Training Area/Old Sanitary Landfill Area ( FTA /OSLA), which encompasses 28 acres, five of

  9. An evaluation of surgical outcome of bilateral cleft lip surgery using a modified Millard′s (Fork Flap technique

    Directory of Open Access Journals (Sweden)

    W L Adeyemo

    2013-01-01

    Full Text Available Background: The central third of the face is distorted by the bilateral cleft of the lip and palate and restoring the normal facial form is one of the primary goals for the reconstructive surgeons. The history of bilateral cleft lip repair has evolved from discarding the premaxilla and prolabium and approximating the lateral lip elements to a definitive lip and primary cleft nasal repair utilising the underlying musculature. The aim of this study was to review surgical outcome of bilateral cleft lip surgery (BCLS done at the Lagos University Teaching Hospital. Materials and Methods: A review of all cases of BCLS done between January 2007 and December 2012 at the Lagos University Teaching Hospital was done. Data analysis included age and sex of patients, type of cleft deformity and type of surgery (primary or secondary and whether the cleft deformity was syndromic and non-syndromic. Techniques of repair, surgical outcome and complications were also recorded. Results: A total of 39 cases of BCLS involving 21 males and 18 females were done during the period. This constituted 10% (39/390 of all cases of cleft surgery done during the period. There were 5 syndromic and 34 non-syndromic cases. Age of patients at time of surgery ranged between 3 months and 32 years. There were 24 bilateral cleft lip and palate deformities and 15 bilateral cleft lip deformities. Thirty-one of the cases were primary surgery, while 8 were secondary (revision surgery. The most common surgical technique employed was modified Fork flap (Millard technique, which was employed in 37 (95% cases. Conclusion: Bilateral cleft lip deformity is a common cleft deformity seen in clinical practice, surgical repair of which can be a challenge to an experienced surgeon. A modified Fork flap technique for repair of bilateral cleft lip is a reliable and versatile technique associated with excellent surgical outcome.

  10. South Fork Snake River/Palisades Wildlife Mitigation Project: Environmental assessment

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-09-01

    BPA proposes to fund the implementation of the South Fork Snake River Programmatic Management Plan to compensate for losses of wildlife and wildlife habitat due to hydroelectric development at Palisades Dam. The Idaho Department of Fish and Game drafted the plan, which was completed in May 1993. This plan recommends land and conservation easement acquisition and wildlife habitat enhancement measures. These measures would be implemented on selected lands along the South Fork of the Snake River between Palisades Dam and the confluence with the Henry`s Fork, and on portions of the Henry`s Fork located in Bonneville, Madison, and Jefferson Counties, Idaho. BPA has prepared an Environmental Assessment evaluating the proposed project. The EA also incorporates by reference the analyses in the South Fork Snake River Activity/Operations Plan and EA prepared jointly in 1991 by the Bureau of Land Management and the Forest Service. Based on the analysis in the EA, BPA has determined that the proposed action is not a major Federal action significantly affecting the quality of the human environment within the meaning of the National Environmental Policy Act (NEPA) of 1969. Therefore, the preparation of an Environmental Impact Statement (EIS) is not required and BPA is issuing this FONSI.

  11. DNA Copy-Number Control through Inhibition of Replication Fork Progression

    Directory of Open Access Journals (Sweden)

    Jared T. Nordman

    2014-11-01

    Full Text Available Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, specific regions of the genome are repressed for DNA replication during differentiation by the SNF2 domain-containing protein SUUR through an unknown mechanism. We demonstrate that SUUR is recruited to active replication forks and mediates the repression of DNA replication by directly inhibiting replication fork progression instead of functioning as a replication fork barrier. Mass spectrometry identification of SUUR-associated proteins identified the replicative helicase member CDC45 as a SUUR-associated protein, supporting a role for SUUR directly at replication forks. Our results reveal that control of eukaryotic DNA copy number can occur through the inhibition of replication fork progression.

  12. Mechanically stable tuning fork sensor with high quality factor for the atomic force microscope.

    Science.gov (United States)

    Kim, Kwangyoon; Park, Jun-Young; Kim, K B; Lee, Naesung; Seo, Yongho

    2014-01-01

    A quartz tuning fork was used instead of cantilever as a force sensor for the atomic force microscope. A tungsten tip was made by electrochemical etching from a wire of 50 µm diameter. In order to have mechanical stability of the tuning fork, it was attached on an alumina plate. The tungsten tip was attached on the inside end of a prong of a tuning fork. The phase shift was used as a feedback signal to control the distance between the tip and sample, and the amplitude was kept constant using a lock-in amplifier and a homemade automatic gain controller. Due to the mechanical stability, the sensor shows a high quality factor (∼10(3)), and the image quality obtained with this sensor was equivalent to that of the cantilever-based AFM. © 2014 Wiley Periodicals, Inc.

  13. The Coefficient of the Voltage Induced Frequency Shift Measurement on a Quartz Tuning Fork

    Directory of Open Access Journals (Sweden)

    Yubin Hou

    2014-11-01

    Full Text Available We have measured the coefficient of the voltage induced frequency shift (VIFS of a 32.768 KHz quartz tuning fork. Three vibration modes were studied: one prong oscillating, two prongs oscillating in the same direction, and two prongs oscillating in opposite directions. They all showed a parabolic dependence of the eigen-frequency shift on the bias voltage applied across the fork, due to the voltage-induced internal stress, which varies as the fork oscillates. The average coefficient of the VIFS effect is as low as several hundred nano-Hz per millivolt, implying that fast-response voltage-controlled oscillators and phase-locked loops with nano-Hz resolution can be built.

  14. Chemical bond imaging using higher eigenmodes of tuning fork sensors in atomic force microscopy

    Science.gov (United States)

    Ebeling, Daniel; Zhong, Qigang; Ahles, Sebastian; Chi, Lifeng; Wegner, Hermann A.; Schirmeisen, André

    2017-05-01

    We demonstrate the ability of resolving the chemical structure of single organic molecules using non-contact atomic force microscopy with higher normal eigenmodes of quartz tuning fork sensors. In order to achieve submolecular resolution, CO-functionalized tips at low temperatures are used. The tuning fork sensors are operated in ultrahigh vacuum in the frequency modulation mode by exciting either their first or second eigenmode. Despite the high effective spring constant of the second eigenmode (on the order of several tens of kN/m), the force sensitivity is sufficiently high to achieve atomic resolution above the organic molecules. This is observed for two different tuning fork sensors with different tip geometries (small tip vs. large tip). These results represent an important step towards resolving the chemical structure of single molecules with multifrequency atomic force microscopy techniques where two or more eigenmodes are driven simultaneously.

  15. 33 CFR 165.552 - Security Zone; Oyster Creek Generation Station, Forked River, Ocean County, New Jersey.

    Science.gov (United States)

    2010-07-01

    ... Generation Station, Forked River, Ocean County, New Jersey. 165.552 Section 165.552 Navigation and Navigable... Coast Guard District § 165.552 Security Zone; Oyster Creek Generation Station, Forked River, Ocean... part. (2) No person or vessel may enter or navigate within this security zone unless authorized to do...

  16. Integrating Salmon Recovery, Clean Water Act Compliance, Restoration, and Climate Change Impacts in the South Fork Nooksack River

    Science.gov (United States)

    "The South Fork Nooksack River (SFNR) is an important tributary to the Nooksack River, Bellingham Bay, and the Salish Sea. The South Fork Nooksack River comprises one of the 22 independent populations of spring Chinook in the Puget Sound Chinook Evolutionarily Significant Un...

  17. Sediment transport and storage in North Fork Caspar Creek, Mendocino County, California: water years 1980-1988

    Science.gov (United States)

    Michael Brent Napolitano

    1996-01-01

    Abstract - The old-growth redwood forest of North Fork Caspar Creek was clear-cut between 1864 and 1904. Previous research on logging-related changes in suspended sediment and streamflow would suggest that North Fork Caspar Creek has recovered from historical logging (Rice et al., 1979; Ziemer, 1981); research on the influence of large woody debris (LWD) on channel...

  18. Reduced fuel consumption for fork-lift trucks with hydrostatic transmission

    Energy Technology Data Exchange (ETDEWEB)

    Abels, T

    1983-05-01

    Cost calculations for a 3,5-t diesel fork lifter done on the basis of VDI 2695 shows, that fuel costs account only for a small part of the operating costs despite the price increase for diesel fuel. Fork lifters with disk-cam controlled primary/secondary adjusted hydrostatic transmission used less fuel than was indicated in the VDI-guideline. Fuel consumption could further be reduced by an optimized hydraulic adjustment together with a precisely harmonized engine speed adjustment. Annual cost savings are considerable.

  19. Burnup verification tests with the FORK measurement system-implementation for burnup credit

    International Nuclear Information System (INIS)

    Ewing, R.I.

    1994-01-01

    Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. It was designed at Los Alamos National Laboratory for the International Atomic Energy Agency safeguards program and is well suited to verify burnup and cooling time records at commercial Pressurized Water Reactor (PWR) sites. This report deals with the application of the FORK system to burnup credit operations

  20. Metamorphism and plutonism around the middle and south forks of the Feather River, California

    Science.gov (United States)

    Hietanen, Anna Martta

    1976-01-01

    The area around the Middle and South Forks of the Feather River provides information on metamorphic and igneous processes that bear on the origin of andesitic and granitic magmas in general and on the variation of their potassium content in particular. In the north, the area joins the Pulga and Bucks Lake quadrangles studied previously. Tectonically, this area is situated in the southern part of an arcuate segment of the Nevadan orogenic belt in the northwestern Sierra Nevada. The oldest rocks are metamorphosed calcalkaline island-arc-type andesite, dacite, and sodarhyolite with interbedded tuff layers (the Franklin Canyon Formation), all probably correlative with Devonian rocks in the Klamath Mountains. Younger rocks form a sequence of volcanic, volcaniclastic, and sedimentary rocks including some limestone (The Horseshoe Bend Formation), probably Permian in age. All the volcanic and sedimentary rocks were folded and recrystallized to the greenschist facies during the Nevadan (Jurassic) orogeny and were invaded by monzotonalitic magmas shortly thereafter. A second lineation and metamorphism to the epidote-amphibolite facies developed in a narrow zone around the plutons. In light of the concept of plate tectonics, it is suggested that the early (Devonian?) island-arc-type andesite, dacite, and sodarhyolite (the Franklin Canyon Formation) were derived from the mantle above a Benioff zone by partial melting of peridotite in hydrous conditions. The water was probably derived from an oceanic plate descending to the mantle. Later (Permian?) magmas were mainly basaltic; some discontinuous layers of potassium-rich rhyolite indicate a change into anhydrous conditions and a deeper level of magma generation. The plutonic magmas that invaded the metamorphic rocks at the end of the Jurassic may contain material from the mantle, the subducted oceanic lithosphere, and the downfolded metamorphic rocks. The ratio of partial melts from these three sources may have changed with time

  1. 77 FR 47058 - Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft...

    Science.gov (United States)

    2012-08-07

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [P-2079-069--CA] Middle Fork American River Hydroelectric Project Placer County Water Agency; Notice of Draft Environmental Impact Statement; Public Meetings a. Date and Time of Meetings: Tuesday, August 28, 2012, from 9:00 a.m. to 11:00 a.m. and...

  2. Plant composition in oak savanna and woodland restoration at Prairie Fork Conservation Area in Missouri

    Science.gov (United States)

    Nadia E. Navarrete-Tindall; J.W. Van Sambeek; Jamie Coe; Warren Taylor

    2007-01-01

    The wooded areas of the Prairie Fork Conservation Area in central Missouri are typical of the oak/hickory forest/prairie transition zone that will require active management to restore pre-settlement, grass dominated savannas and open woodlands to improve habitat for wildlife. We initiated a management program to restore savannas and woodlands by reducing the midstory (...

  3. Stream bank and sediment movement associated with 2008 flooding, South Fork Iowa River

    Science.gov (United States)

    Stream bank erosion can cause substantial damage to riparian systems and impact the use of water downstream. Risks of bank erosion increase during extreme flood events, and frequencies of extreme events may be increasing under changing climate. We assessed bank erosion within the South Fork Iowa Riv...

  4. 76 FR 6114 - Lincoln National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization

    Science.gov (United States)

    2011-02-03

    ... National Forest, New Mexico, North Fork Eagle Creek Wells Special Use Authorization AGENCY: Forest Service, USDA. ACTION: Notice of Intent to prepare an Environmental Impact Statement. SUMMARY: The Lincoln National Forest will prepare an Environmental Impact Statement (EIS) to document and publicly disclose...

  5. Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function.

    Science.gov (United States)

    Dijkstra, Anke A; Lin, Li-Chun; Nana, Alissa L; Gaus, Stephanie E; Seeley, William W

    2018-01-01

    The human anterior cingulate and frontoinsular cortices are distinguished by 2 unique Layer 5 neuronal morphotypes, the von Economo neurons (VENs) and fork cells, whose biological identity remains mysterious. Insights could impact research on diverse neuropsychiatric diseases to which these cells have been linked. Here, we leveraged the Allen Brain Atlas to evaluate mRNA expression of 176 neurotransmitter-related genes and identified vesicular monoamine transporter 2 (VMAT2), gamma-aminobutyric acid (GABA) receptor subunit θ (GABRQ), and adrenoreceptor α-1A (ADRA1A) expression in human VENs, fork cells, and a minority of neighboring Layer 5 neurons. We confirmed these results using immunohistochemistry or in situ hybridization. VMAT2 and GABRQ expression was absent in mouse cerebral cortex. Although VMAT2 is known to package monoamines into synaptic vesicles, in VENs and fork cells its expression occurs in the absence of monoamine-synthesizing enzymes or reuptake transporters. Thus, VENs and fork cells may possess a novel, uncharacterized mode of cortical monoaminergic function that distinguishes them from most other mammalian Layer 5 neurons. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. The progression of replication forks at natural replication barriers in live bacteria

    NARCIS (Netherlands)

    Moolman, M.C.; Tiruvadi Krishnan, S; Kerssemakers, J.W.J.; de Leeuw, R.; Lorent, V.J.F.; Sherratt, David J.; Dekker, N.H.

    2016-01-01

    Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these

  7. Performance Evaluation of the New Fork-Absorbers of RSG-GAS Control Rod

    International Nuclear Information System (INIS)

    Slamet Wiranto; Purwadi; Arif Hidayat; Agus Sanjaya

    2012-01-01

    During the operation of RSG-GAS reactor, it has been replaced 8 fork-absorber by the new absorber from PT. Batan Teknologi. After almost 5 years under utilization it is important to be evaluated to determine the physical condition and its performance, which is still in good condition and functioning according to the requirements of its operations. The evaluation has been carried out by studying and analyzing the data of the fork-absorber utilization in the the reactor core. The fork absorber data consist of visual inspection, control rod drop time measurement and control rod reactivity and safety margin measurement for each operation cycle. Through the observation up to date with the operating cycle of 79, could be concluded that the fork-absorber condition is still good, and has ability, to support the operation until ± 660 MWD/cycle, which is characterized by obtaining the value of ρ-excess is sufficient for operation, with a large safety margin. (author)

  8. "Forked Tongue: The Politics of Bilingual Education," by Rosalie Pedalino Porter. Book Review.

    Science.gov (United States)

    Amabisca, Anastasia Aimee

    1994-01-01

    Based on personal experience rather than empirical evidence, "Forked Tongue" presents a biased view that could damage the future of bilingual education in the United States. The book considers bilingual education a threat because it prolongs the learning of English and promotes the maintenance of other "un-American" languages…

  9. Mammalian RAD52 Functions in Break-Induced Replication Repair of Collapsed DNA Replication Forks.

    Science.gov (United States)

    Sotiriou, Sotirios K; Kamileri, Irene; Lugli, Natalia; Evangelou, Konstantinos; Da-Ré, Caterina; Huber, Florian; Padayachy, Laura; Tardy, Sebastien; Nicati, Noemie L; Barriot, Samia; Ochs, Fena; Lukas, Claudia; Lukas, Jiri; Gorgoulis, Vassilis G; Scapozza, Leonardo; Halazonetis, Thanos D

    2016-12-15

    Human cancers are characterized by the presence of oncogene-induced DNA replication stress (DRS), making them dependent on repair pathways such as break-induced replication (BIR) for damaged DNA replication forks. To better understand BIR, we performed a targeted siRNA screen for genes whose depletion inhibited G1 to S phase progression when oncogenic cyclin E was overexpressed. RAD52, a gene dispensable for normal development in mice, was among the top hits. In cells in which fork collapse was induced by oncogenes or chemicals, the Rad52 protein localized to DRS foci. Depletion of Rad52 by siRNA or knockout of the gene by CRISPR/Cas9 compromised restart of collapsed forks and led to DNA damage in cells experiencing DRS. Furthermore, in cancer-prone, heterozygous APC mutant mice, homozygous deletion of the Rad52 gene suppressed tumor growth and prolonged lifespan. We therefore propose that mammalian RAD52 facilitates repair of collapsed DNA replication forks in cancer cells. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  10. Dual parasitism of Fork-tailed Drongos by African and Jacobin ...

    African Journals Online (AJOL)

    Different species of brood parasitic birds, which lay their eggs in the nests of host foster-parents, rarely target the same host species population. We report brood parasitism of Fork-tailed Drongos Dicrurus adsimilis in the southern Kalahari Desert by both African Cuckoo Cuculus gularis and Jacobin Cuckoo Clamator ...

  11. Effects of timber harvest on aquatic vertebrates and habitat in the North Fork Caspar Creek

    Science.gov (United States)

    Rodney J. Nakamoto

    1998-01-01

    I examined the relationships between timber harvest, creek habitat, and vertebrate populations in the North and South forks of Caspar Creek. Habitat inventories suggested pool availability increased after the onset of timber harvest activities. Increased large woody debris in the channel was associated with an increase in the frequency of blowdown in the riparian...

  12. Storm water control plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This document provides the Environmental Restoration Program with information about the erosion and sediment control, storm water management, maintenance, and reporting and record keeping practices to be employed during Phase II of the remediation project for the Lower East Fork Poplar Creek (LEFPC) Operable Unit

  13. 76 FR 20463 - Endangered and Threatened Wildlife and Plants; Proposed Endangered Status for the Three Forks...

    Science.gov (United States)

    2011-04-12

    ... precariously, at Three Forks Springs. Lack of vegetation and forest litter following intense crown fires can... Policy and Directives Management; U.S. Fish and Wildlife Service; 4401 N. Fairfax Drive, Suite 222; Arlington, VA 22203. We will not accept e-mail or faxes. We will post all comments on http://www.regulations...

  14. Noncontact atomic force microscopy in liquid environment with quartz tuning fork and carbon nanotube probe

    DEFF Research Database (Denmark)

    Kageshima, M.; Jensenius, Henriette; Dienwiebel, M.

    2002-01-01

    A force sensor for noncontact atomic force microscopy in liquid environment was developed by combining a multiwalled carbon nanotube (MWNT) probe with a quartz tuning fork. Solvation shells of octamethylcyclotetrasiloxane surface were detected both in the frequency shift and dissipation. Due to t...

  15. High-affinity DNA-binding Domains of Replication Protein A (RPA) Direct SMARCAL1-dependent Replication Fork Remodeling*

    Science.gov (United States)

    Bhat, Kamakoti P.; Bétous, Rémy; Cortez, David

    2015-01-01

    SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. PMID:25552480

  16. High-affinity DNA-binding domains of replication protein A (RPA) direct SMARCAL1-dependent replication fork remodeling.

    Science.gov (United States)

    Bhat, Kamakoti P; Bétous, Rémy; Cortez, David

    2015-02-13

    SMARCAL1 catalyzes replication fork remodeling to maintain genome stability. It is recruited to replication forks via an interaction with replication protein A (RPA), the major ssDNA-binding protein in eukaryotic cells. In addition to directing its localization, RPA also activates SMARCAL1 on some fork substrates but inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reactions. We investigated the mechanism by which RPA regulates SMARCAL1. Our results indicate that although an interaction between SMARCAL1 and RPA is essential for SMARCAL1 activation, the location of the interacting surface on RPA is not. Counterintuitively, high-affinity DNA binding of RPA DNA-binding domain (DBD) A and DBD-B near the fork junction makes it easier for SMARCAL1 to remodel the fork, which requires removing RPA. We also found that RPA DBD-C and DBD-D are not required for SMARCAL1 regulation. Thus, the orientation of the high-affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes.

    Science.gov (United States)

    Hilton, Benjamin A; Liu, Ji; Cartwright, Brian M; Liu, Yiyong; Breitman, Maya; Wang, Youjie; Jones, Rowdy; Tang, Hui; Rusinol, Antonio; Musich, Phillip R; Zou, Yue

    2017-09-01

    Hutchinson-Gilford progeria syndrome (HGPS) is a rare genetic disorder that is caused by a point mutation in the LMNA gene, resulting in production of a truncated farnesylated-prelamin A protein (progerin). We previously reported that XPA mislocalized to the progerin-induced DNA double-strand break (DSB) sites, blocking DSB repair, which led to DSB accumulation, DNA damage responses, and early replication arrest in HGPS. In this study, the XPA mislocalization to DSBs occurred at stalled or collapsed replication forks, concurrent with a significant loss of PCNA at the forks, whereas PCNA efficiently bound to progerin. This PCNA sequestration likely exposed ds-ssDNA junctions at replication forks for XPA binding. Depletion of XPA or progerin each significantly restored PCNA at replication forks. Our results suggest that although PCNA is much more competitive than XPA in binding replication forks, PCNA sequestration by progerin may shift the equilibrium to favor XPA binding. Furthermore, we demonstrated that progerin-induced apoptosis could be rescued by XPA, suggesting that XPA-replication fork binding may prevent apoptosis in HGPS cells. Our results propose a mechanism for progerin-induced genome instability and accelerated replicative senescence in HGPS.-Hilton, B. A., Liu, J., Cartwright, B. M., Liu, Y., Breitman, M., Wang, Y., Jones, R., Tang, H., Rusinol, A., Musich, P. R., Zou, Y. Progerin sequestration of PCNA promotes replication fork collapse and mislocalization of XPA in laminopathy-related progeroid syndromes. © FASEB.

  18. Simple force balance accelerometer/seismometer based on a tuning fork displacement sensor

    International Nuclear Information System (INIS)

    Stuart-Watson, D.; Tapson, J.

    2004-01-01

    Seismometers and microelectromechanical system accelerometers use the force-balance principle to obtain measurements. In these instruments the displacement of a mass object by an unknown force is sensed using a very high-resolution displacement sensor. The position of the object is then stabilized by applying an equal and opposite force to it. The magnitude of the stabilizing force is easily measured, and is assumed to be equivalent to the unknown force. These systems are critically dependent on the displacement sensor. In this article we use a resonant quartz tuning fork as the sensor. The tuning fork is operated so that its oscillation is lightly damped by the proximity of the movable mass object. Changes in the position of the mass object cause changes in the phase of the fork's resonance; this is used as the feedback variable in controlling the mass position. We have developed an acceleration sensor using this principle. The mass object is a piezoelectric bimorph diaphragm which is anchored around its perimeter, allowing direct electronic control of the displacement of its center. The tuning fork is brought very close to the diaphragm center, and is connected into a self-oscillating feedback circuit which has phase and amplitude as outputs. The diaphragm position is adjusted by a feedback loop, using phase as the feedback variable, to keep it in a constant position with respect to the tuning fork. The measured noise for this sensor is approximately 10.0 mg in a bandwidth of 100 Hz, which is substantially better than commercial systems of equivalent cost and size

  19. Evaluation of Lower East Fork Poplar Creek Mercury Sources

    International Nuclear Information System (INIS)

    Watson, David B.; Brooks, Scott C.; Mathews, Teresa J.; Bevelhimer, Mark S.; DeRolph, Chris; Brandt, Craig C.; Peterson, Mark J.; Ketelle, Richard

    2016-01-01

    This report summarizes a 3-year research project undertaken to better understand the nature and magnitude of mercury (Hg) fluxes in East Fork Poplar Creek (EFPC). This project addresses the requirements of Action Plan 1 in the 2011 Oak Ridge Reservation-wide Comprehensive Environmental Response, Compensation, and Liability Act Five Year Review (FYR). The Action Plan is designed to address a twofold 2011 FYR issue: (1) new information suggests mobilization of mercury from the upper and lower EFPC streambeds and stream banks is the primary source of mercury export during high-flow conditions, and (2) the current Record of Decision did not address the entire hydrologic system and creek bank or creek bed sediments. To obtain a more robust watershed-scale understanding of mercury sources and processes in lower EFPC (LEFPC), new field and laboratory studies were coupled with existing data from multiple US Department of Energy programs to develop a dynamic watershed and bioaccumulation model. LEFPC field studies for the project focused primarily on quantification of streambank erosion and an evaluation of mercury dynamics in shallow groundwater adjacent to LEFPC and potential connection to the surface water. The approach to the stream bank study was innovative in using imagery from kayak floats' surveys from the headwaters to the mouth of EFPC to estimate erosion, coupled with detailed bank soil mercury analyses. The goal of new field assessments and modeling was to generate a more holistic and quantitative understanding of the watershed and the sources, flux, concentration, transformation, and bioaccumulation of inorganic mercury (IHg) and methylmercury (MeHg). Model development used a hybrid approach that dynamically linked a spreadsheet-based physical and chemical watershed model to a systems dynamics, mercury bioaccumulation model for key fish species. The watershed model tracks total Hg and MeHg fluxes and concentrations by examining upstream inputs, floodplain

  20. Evaluation of Lower East Fork Poplar Creek Mercury Sources

    Energy Technology Data Exchange (ETDEWEB)

    Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bevelhimer, Mark S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Chris [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Ketelle, Richard [East Tennessee Technology Park (ETTP), Oak Ridge, TN (United States)

    2016-06-01

    This report summarizes a 3-year research project undertaken to better understand the nature and magnitude of mercury (Hg) fluxes in East Fork Poplar Creek (EFPC). This project addresses the requirements of Action Plan 1 in the 2011 Oak Ridge Reservation-wide Comprehensive Environmental Response, Compensation, and Liability Act Five Year Review (FYR). The Action Plan is designed to address a twofold 2011 FYR issue: (1) new information suggests mobilization of mercury from the upper and lower EFPC streambeds and stream banks is the primary source of mercury export during high-flow conditions, and (2) the current Record of Decision did not address the entire hydrologic system and creek bank or creek bed sediments. To obtain a more robust watershed-scale understanding of mercury sources and processes in lower EFPC (LEFPC), new field and laboratory studies were coupled with existing data from multiple US Department of Energy programs to develop a dynamic watershed and bioaccumulation model. LEFPC field studies for the project focused primarily on quantification of streambank erosion and an evaluation of mercury dynamics in shallow groundwater adjacent to LEFPC and potential connection to the surface water. The approach to the stream bank study was innovative in using imagery from kayak floats’ surveys from the headwaters to the mouth of EFPC to estimate erosion, coupled with detailed bank soil mercury analyses. The goal of new field assessments and modeling was to generate a more holistic and quantitative understanding of the watershed and the sources, flux, concentration, transformation, and bioaccumulation of inorganic mercury (IHg) and methylmercury (MeHg). Model development used a hybrid approach that dynamically linked a spreadsheet-based physical and chemical watershed model to a systems dynamics, mercury bioaccumulation model for key fish species. The watershed model tracks total Hg and MeHg fluxes and concentrations by examining upstream inputs, floodplain

  1. Flood-inundation maps for the East Fork White River at Columbus, Indiana

    Science.gov (United States)

    Lombard, Pamela J.

    2013-01-01

    Digital flood-inundation maps for a 5.4-mile reach of the East Fork White River at Columbus, Indiana, from where the Flatrock and Driftwood Rivers combine to make up East Fork White River to just upstream of the confluence of Clifty Creek with the East Fork White River, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent of flooding corresponding to selected water levels (stages) at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. Current conditions at the USGS streamgage may be obtained on the Internet from the USGS National Water Information System (http://waterdata.usgs.gov/in/nwis/uv/?site_no=03364000&agency_cd=USGS&). The National Weather Service (NWS) forecasts flood hydrographs for the East Fork White River at Columbus, Indiana at their Advanced Hydrologic Prediction Service (AHPS) flood warning system Website (http://water.weather.gov/ahps/), that may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the stream reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relation at USGS streamgage 03364000, East Fork White River at Columbus, Indiana. The calibrated hydraulic model was then used to determine 15 water-surface profiles for flood stages at 1-foot (ft) intervals referenced to the streamgage datum and ranging from bankfull to approximately the highest recorded water level at the streamgage. The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from Light Detection and Ranging (LiDAR) data), having a 0.37-ft vertical accuracy and a 1.02 ft

  2. Environmental Assessment - Construct a Ground-to-Air Transmitter and Receiver (GATR) Facility at Grand Forks Air Force Base

    National Research Council Canada - National Science Library

    2006-01-01

    ...) facility on Grand Forks Air Force Base (AFB), North Dakota. The Communication Squadron is preparing to install new GATR communication antennas and systems, for tactical aircraft control and commercial air traffic control...

  3. Seasonal Alterations in Park Visitation, Amenity Use, and Physical Activity — Grand Forks, North Dakota, 2012–2013

    Science.gov (United States)

    Johnson, LuAnn

    2014-01-01

    Introduction Park amenities promote visitation and physical activity during summer. Physical activity declines during winter. Identifying park amenities that promote visitation during winter would increase year-round activity. The purpose of this study was to determine how park visitation, amenity choice, and physical activity intensity change across seasons. Methods Physical activity intensity of children and adults was assessed at 16 parks in Grand Forks, North Dakota, during summer and fall of 2012, and winter and spring of 2013. Results Park visitation was highest in spring and lowest in winter. Amenity use varied by season. Parks with water splash pads were visited more during summer, and playgrounds and open spaces were visited more during spring. Ice rinks were visited most in winter. Physical activity intensity was lowest in summer and highest in winter for each age group. The activity intensity observed for all young age groups ranged from 2.7 to 2.9 metabolic equivalents in summer and greater than 3 metabolic equivalents in all other seasons. Adults’ mean activity intensity was greater than 3 metabolic equivalents in winter. Conclusion Information on park visitation, amenity use, and activity intensity across seasons is valuable; it can be used when designing or redesigning parks in order to promote year-round physical activity. Redesigning parks in cold climates to include ice rinks, sledding hills, cross-country skiing, and indoor areas for physical activity would increase winter visitation and allow the park to serve as a year-round resource for physical activity. PMID:25211503

  4. Seasonal alterations in park visitation, amenity use, and physical activity--Grand Forks, North Dakota, 2012-2013.

    Science.gov (United States)

    Roemmich, James N; Johnson, LuAnn

    2014-09-11

    Park amenities promote visitation and physical activity during summer. Physical activity declines during winter. Identifying park amenities that promote visitation during winter would increase year-round activity. The purpose of this study was to determine how park visitation, amenity choice, and physical activity intensity change across seasons. Physical activity intensity of children and adults was assessed at 16 parks in Grand Forks, North Dakota, during summer and fall of 2012, and winter and spring of 2013. Park visitation was highest in spring and lowest in winter. Amenity use varied by season. Parks with water splash pads were visited more during summer, and playgrounds and open spaces were visited more during spring. Ice rinks were visited most in winter. Physical activity intensity was lowest in summer and highest in winter for each age group. The activity intensity observed for all young age groups ranged from 2.7 to 2.9 metabolic equivalents in summer and greater than 3 metabolic equivalents in all other seasons. Adults' mean activity intensity was greater than 3 metabolic equivalents in winter. Information on park visitation, amenity use, and activity intensity across seasons is valuable; it can be used when designing or redesigning parks in order to promote year-round physical activity. Redesigning parks in cold climates to include ice rinks, sledding hills, cross-country skiing, and indoor areas for physical activity would increase winter visitation and allow the park to serve as a year-round resource for physical activity.

  5. Age distribution and relationship between fork length and otolith length of bluefish (Pomatomus saltatrix

    Directory of Open Access Journals (Sweden)

    Tevfik Ceyhan

    2015-12-01

    Full Text Available Marmara Denizi’nden elde edilmiş lüferler (Pomatomus saltatrix L.,1766 yaş okumaları ve balık boyu-otolit boyu ilişkisi için incelenmiştir. Toplam 688 sagittal otolit dijital bir kumpasla anterior-posterior doğrultusunda ölçülmüştür. En büyük otolit boyu 10,1 mm’ye karşın maksimum balık boyu 342 mm’dir. Doğal olarak otolit boyu balık boyuyla artmaktadır

  6. Turbo Charge CPU Utilization in Fork/Join Using the ManagedBlocker

    CERN Multimedia

    CERN. Geneva

    2017-01-01

    Fork/Join is a framework for parallelizing calculations using recursive decomposition, also called divide and conquer. These algorithms occasionally end up duplicating work, especially at the beginning of the run. We can reduce wasted CPU cycles by implementing a reserved caching scheme. Before a task starts its calculation, it tries to reserve an entry in the shared map. If it is successful, it immediately begins. If not, it blocks until the other thread has finished its calculation. Unfortunately this might result in a significant number of blocked threads, decreasing CPU utilization. In this talk we will demonstrate this issue and offer a solution in the form of the ManagedBlocker. Combined with the Fork/Join, it can keep parallelism at the desired level.

  7. Inhibition of DNA chain elongation in Chinese hamster cells by damage localized behind the replication fork

    Energy Technology Data Exchange (ETDEWEB)

    Ben-Hur, E [Israel Atomic Energy Commission, Beersheba. Nuclear Research Center-Negev; Hagan, M P [Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)

    1984-05-01

    Chinese hamster fibroblasts were pulse labelled with 5-bromodeoxyuridine and exposed at time intervals (Tsub(i)) to near-ultraviolet (U.V.A.) light in the presence of a bisbenzimidazole derivative (Hoechst 33342). The sensitivity of the cells in terms of colony forming ability fluctuated depending on Tsub(i). Inhibition of DNA synthesis also depended on Tsub(i) and was maximal when Tsub(i)=O. Using the alkaline elution technique it was shown that the effect of a large dose of light was to inhibit both initiation and elongation of DNA chains. These effects were most pronounced for Tsub(i)=O. It is concluded that DNA damage in an active replicon can inhibit initiation of new replicons and that damage localized behind the replication fork can retard elongation of nascent DNA chains. This effect on chain elongation decreases with increased distance of the damage from the replication fork.

  8. Recombination at DNA replication fork barriers is not universal and is differentially regulated by Swi1.

    Science.gov (United States)

    Pryce, David W; Ramayah, Soshila; Jaendling, Alessa; McFarlane, Ramsay J

    2009-03-24

    DNA replication stress has been implicated in the etiology of genetic diseases, including cancers. It has been proposed that genomic sites that inhibit or slow DNA replication fork progression possess recombination hotspot activity and can form potential fragile sites. Here we used the fission yeast, Schizosaccharomyces pombe, to demonstrate that hotspot activity is not a universal feature of replication fork barriers (RFBs), and we propose that most sites within the genome that form RFBs do not have recombination hotspot activity under nonstressed conditions. We further demonstrate that Swi1, the TIMELESS homologue, differentially controls the recombination potential of RFBs, switching between being a suppressor and an activator of recombination in a site-specific fashion.

  9. Mercury Content of Sediments in East Fork Poplar Creek: Current Assessment and Past Trends

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Eller, Virginia A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Earles, Jennifer E. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lowe, Kenneth Alan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mehlhorn, Tonia L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Watson, David J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Phillips, Debra H. [Queen' s Univ., Belfast (United Kingdom); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-01-01

    This study provided new information on sediment mercury (Hg) and monomethylmercury (MMHg) content and chemistry. The current inventory of Hg in East Fork Poplar Creek (EFPC) bed sediments was estimated to be 334 kg, which represents a ~67% decrease relative to the initial investigations in 1984. MMHg sediment inventory was estimated to be 44.1 g, lower but roughly similar to past estimates. The results support the relevance and potential impacts of other active and planned investigations within the Mercury Remediation Technology Development for Lower East Fork Poplar Creek project (e.g., assessment and control of bank soil inputs, sorbents for Hg and MMHg removal, re-introduction of freshwater clams to EFPC), and identify gaps in current understanding that represent opportunities to understand controlling variables that may inform future technology development studies.

  10. Temperature, Frequency, and Young’s Modulus of an Aluminum Tuning Fork

    Directory of Open Access Journals (Sweden)

    Zachery L. Greer

    2011-01-01

    Full Text Available The frequency produced by a standard C (523.3 Hz aluminum alloy tuning fork when struck at temperatures ranging from 29 ̊C to 300 ̊C was studied. It was found that frequency decreased with increasing temperature with an inverse exponential relationship. The frequency was used to calculate Young’s Modulus for aluminum, with the results being in close agreement with published values.

  11. Gaussian Random Fields Methods for Fork-Join Network with Synchronization Constraints

    Science.gov (United States)

    2014-12-22

    substantial efforts were dedicated to the study of the max-plus recursions [21, 3, 12]. More recently, Atar et al. [2] have studied a fork-join...feedback and NES, Atar et al. [2] show that a dynamic priority discipline achieves throughput optimal- ity asymptotically in the conventional heavy...2011) Patient flow in hospitals: a data-based queueing-science perspective. Submitted to Stochastic Systems, 20. [2] R. Atar , A. Mandelbaum and A

  12. Human Costs Assessment - The Impacts of Flooding & Nonstructural Solutions. Tug Fork Valley, West Virginia & Kentucky.

    Science.gov (United States)

    1980-04-01

    to the outside world (especially in the West Virginia side). New people are being drawn in and there is a continuing out migration. Franchised ...all of these businesses are local ones suggesting the skillful entrepreneurship of area people and the recognition of Goody’s existence in the larger...chain stores and franchise businesses. The net effect is that here is another instance in which the Tug Fork people are dependent upon outsiders. King

  13. Escherichia coli DinB inhibits replication fork progression without significantly inducing the SOS response.

    Science.gov (United States)

    Mori, Tetsuya; Nakamura, Tatsuro; Okazaki, Naoto; Furukohri, Asako; Maki, Hisaji; Akiyama, Masahiro Tatsumi

    2012-01-01

    The SOS response is readily triggered by replication fork stalling caused by DNA damage or a dysfunctional replicative apparatus in Escherichia coli cells. E. coli dinB encodes DinB DNA polymerase and its expression is upregulated during the SOS response. DinB catalyzes translesion DNA synthesis in place of a replicative DNA polymerase III that is stalled at a DNA lesion. We showed previously that DNA replication was suppressed without exogenous DNA damage in cells overproducing DinB. In this report, we confirm that this was due to a dose-dependent inhibition of ongoing replication forks by DinB. Interestingly, the DinB-overproducing cells did not significantly induce the SOS response even though DNA replication was perturbed. RecA protein is activated by forming a nucleoprotein filament with single-stranded DNA, which leads to the onset of the SOS response. In the DinB-overproducing cells, RecA was not activated to induce the SOS response. However, the SOS response was observed after heat-inducible activation in strain recA441 (encoding a temperature-sensitive RecA) and after replication blockage in strain dnaE486 (encoding a temperature-sensitive catalytic subunit of the replicative DNA polymerase III) at a non-permissive temperature when DinB was overproduced in these cells. Furthermore, since catalytically inactive DinB could avoid the SOS response to a DinB-promoted fork block, it is unlikely that overproduced DinB takes control of primer extension and thus limits single-stranded DNA. These observations suggest that DinB possesses a feature that suppresses DNA replication but does not abolish the cell's capacity to induce the SOS response. We conclude that DinB impedes replication fork progression in a way that does not activate RecA, in contrast to obstructive DNA lesions and dysfunctional replication machinery.

  14. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress

    Science.gov (United States)

    Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S.

    2016-01-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  15. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress.

    Directory of Open Access Journals (Sweden)

    Therese Wilhelm

    2016-05-01

    Full Text Available Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es. Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3% rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing, and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and

  16. Slow Replication Fork Velocity of Homologous Recombination-Defective Cells Results from Endogenous Oxidative Stress.

    Science.gov (United States)

    Wilhelm, Therese; Ragu, Sandrine; Magdalou, Indiana; Machon, Christelle; Dardillac, Elodie; Técher, Hervé; Guitton, Jérôme; Debatisse, Michelle; Lopez, Bernard S

    2016-05-01

    Replications forks are routinely hindered by different endogenous stresses. Because homologous recombination plays a pivotal role in the reactivation of arrested replication forks, defects in homologous recombination reveal the initial endogenous stress(es). Homologous recombination-defective cells consistently exhibit a spontaneously reduced replication speed, leading to mitotic extra centrosomes. Here, we identify oxidative stress as a major endogenous source of replication speed deceleration in homologous recombination-defective cells. The treatment of homologous recombination-defective cells with the antioxidant N-acetyl-cysteine or the maintenance of the cells at low O2 levels (3%) rescues both the replication fork speed, as monitored by single-molecule analysis (molecular combing), and the associated mitotic extra centrosome frequency. Reciprocally, the exposure of wild-type cells to H2O2 reduces the replication fork speed and generates mitotic extra centrosomes. Supplying deoxynucleotide precursors to H2O2-exposed cells rescued the replication speed. Remarkably, treatment with N-acetyl-cysteine strongly expanded the nucleotide pool, accounting for the replication speed rescue. Remarkably, homologous recombination-defective cells exhibit a high level of endogenous reactive oxygen species. Consistently, homologous recombination-defective cells accumulate spontaneous γH2AX or XRCC1 foci that are abolished by treatment with N-acetyl-cysteine or maintenance at 3% O2. Finally, oxidative stress stimulated homologous recombination, which is suppressed by supplying deoxynucleotide precursors. Therefore, the cellular redox status strongly impacts genome duplication and transmission. Oxidative stress should generate replication stress through different mechanisms, including DNA damage and nucleotide pool imbalance. These data highlight the intricacy of endogenous replication and oxidative stresses, which are both evoked during tumorigenesis and senescence initiation

  17. NDA measurements on spent fuel assemblies at Tihange 1 by means of the ION 1/FORK

    International Nuclear Information System (INIS)

    Carchon, R.; Smaers, G.; Verrecchia, G.P.D.; Arlt, R.; Stoyanova, I.; Satinet, J.

    1986-06-01

    This report describes field tests performed at Tihange 1 Nuclear Power Station on PWR spent fuel by means of the ION 1-FORK detector. Two detector systems and three electronics systems were used to investigate the same fuel assemblies with various burn-ups and cooling times. The purpose of the exercise was to test the performance of the instrument for as well inspection purposes as for fuel management. The results are presented and discussed. (Author)

  18. Burnup verification measurements at a US nuclear utility using the FORK measurement system

    International Nuclear Information System (INIS)

    Ewing, R.I.; Bosler, G.E.; Walden, G.

    1993-01-01

    The FORK measurement system, designed at Los Alamos National Laboratory (LANL) for the International Atomic Energy Agency (IAEA) safeguards program, has been used to examine spent reactor fuel assemblies at Duke Power Company's Oconee Nuclear Station. The FORK system measures the passive neutron and gamma-ray emission from spent fuel assemblies while in the storage pool. These measurements can be correlated with burnup and cooling time, and can be used to verify the reactor site records. Verification measurements may be used to help ensure nuclear criticality safety when burnup credit is applied to spent fuel transport and storage systems. By taking into account the reduced reactivity of spent fuel due to its burnup in the reactor, burnup credit results in more efficient and economic transport and storage. The objectives of these tests are to demonstrate the applicability of the FORK system to verify reactor records and to develop optimal procedures compatible with utility operations. The test program is a cooperative effort supported by Sandia National Laboratories, the Electric Power Research Institute (EPRI), Los Alamos National Laboratory, and the Duke Power Company

  19. MOF Suppresses Replication Stress and Contributes to Resolution of Stalled Replication Forks.

    Science.gov (United States)

    Singh, Dharmendra Kumar; Pandita, Raj K; Singh, Mayank; Chakraborty, Sharmistha; Hambarde, Shashank; Ramnarain, Deepti; Charaka, Vijaya; Ahmed, Kazi Mokim; Hunt, Clayton R; Pandita, Tej K

    2018-03-15

    The human MOF (hMOF) protein belongs to the MYST family of histone acetyltransferases and plays a critical role in transcription and the DNA damage response. MOF is essential for cell proliferation; however, its role during replication and replicative stress is unknown. Here we demonstrate that cells depleted of MOF and under replicative stress induced by cisplatin, hydroxyurea, or camptothecin have reduced survival, a higher frequency of S-phase-specific chromosome damage, and increased R-loop formation. MOF depletion decreased replication fork speed and, when combined with replicative stress, also increased stalled replication forks as well as new origin firing. MOF interacted with PCNA, a key coordinator of replication and repair machinery at replication forks, and affected its ubiquitination and recruitment to the DNA damage site. Depletion of MOF, therefore, compromised the DNA damage repair response as evidenced by decreased Mre11, RPA70, Rad51, and PCNA focus formation, reduced DNA end resection, and decreased CHK1 phosphorylation in cells after exposure to hydroxyurea or cisplatin. These results support the argument that MOF plays an important role in suppressing replication stress induced by genotoxic agents at several stages during the DNA damage response. Copyright © 2018 American Society for Microbiology.

  20. Far-travelled permian chert of the North Fork terrane, Klamath mountains, California

    Science.gov (United States)

    Mankinen, E.A.; Irwin, W.P.; Blome, C.D.

    1996-01-01

    Permian chert in the North Fork terrane and correlative rocks of the Klamath Mountains province has a remanent magnetization that is prefolding and presumably primary. Paleomagnetic results indicate that the chert formed at a paleolatitude of 8.6?? ?? 2.5?? but in which hemisphere remains uncertain. This finding requires that these rocks have undergone at least 8.6?? ?? 4.4?? of northward transport relative to Permian North America since their deposition. Paleontological evidence suggests that the Permian limestone of the Eastern Klamath terrane originated thousands of kilometers distant from North America. The limestone of the North Fork terrane may have formed at a similar or even greater distance as suggested by its faunal affinity to the Eastern Klamath terrane and more westerly position. Available evidence indicates that convergence of the North Fork and composite Central Metamorphic-Eastern Klamath terranes occurred during Triassic or Early Jurassic time and that their joining together was a Middle Jurassic event. Primary and secondary magnetizations indicate that the new composite terrane containing these and other rocks of the Western Paleozoic and Triassic belt behaved as a single rigid block that has been latitudinally concordant with the North American craton since Middle Jurassic time.

  1. The progression of replication forks at natural replication barriers in live bacteria.

    Science.gov (United States)

    Moolman, M Charl; Tiruvadi Krishnan, Sriram; Kerssemakers, Jacob W J; de Leeuw, Roy; Lorent, Vincent; Sherratt, David J; Dekker, Nynke H

    2016-07-27

    Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood. By performing quantitative fluorescence microscopy with microfuidics, we investigate the effect on the replisome when encountering these barriers in live Escherichia coli cells. We make use of an E. coli variant that includes only an ectopic origin of replication that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other replisome. This enables us to single out the effect of encountering a Tus-ter roadblock on an individual replisome. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. These observations demonstrate the resilience of the replication fork to natural barriers and the sensitivity of chromosome alignment to fork progression. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  2. Flood-inundation maps for the East Fork White River near Bedford, Indiana

    Science.gov (United States)

    Fowler, Kathleen K.

    2014-01-01

    Digital flood-inundation maps for an 1.8-mile reach of the East Fork White River near Bedford, Indiana (Ind.) were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selectedwater levels (stages) at USGS streamgage 03371500, East Fork White River near Bedford, Ind. Current conditions for estimating near-real-time areas of inundation using USGS streamgage information may be obtained on the Internet at http://waterdata.usgs.gov/in/nwis/uv?site_no=03371500. In addition, information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service (AHPS) flood warning system (http://water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often colocated with USGS streamgages, including the East Fork White River near Bedford, Ind. NWS-forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. For this study, flood profiles were computed for the East Fork White River reach by means of a one-dimensional step-backwater model. The hydraulic model was calibrated by using the most current stage-discharge relations at USGS streamgage 03371500, East Fork White River near Bedford, Ind., and documented high-water marks from the flood of June 2008. The calibrated hydraulic model was then used to determine 20 water-surface profiles for flood stages at 1-foot intervals referenced to the streamgage datum and ranging from bankfull to the highest stage of the current stage-discharge rating curve. The simulated water-surface profiles were then combined with a geographic information system (GIS) digital elevation model (DEM, derived from

  3. The effectiveness of front fork systems at damping accelerations during isolated aspects specific to cross-country mountain biking.

    Science.gov (United States)

    Macdermid, Paul W; Miller, Matthew C; Fink, Philip W; Stannard, Stephen R

    2017-11-01

    Cross-country mountain bike suspension reportedly enhances comfort and performance through reduced vibration and impact exposure. This study analysed the effectiveness of three different front fork systems at damping accelerations during the crossing of three isolated obstacles (stairs, drop, and root). One participant completed three trials on six separate occasions in a randomised order using rigid, air-sprung, and carbon leaf-sprung forks. Performance was determined by time to cross obstacles, while triaxial accelerometers quantified impact exposure and damping response. Results identified significant main effect of fork type for performance time (p < 0.05). The air-sprung and leaf-sprung forks were significantly slower than the rigid forks for the stairs (p < 0.05), while air-sprung suspension was slower than the rigid for the root protocol (p < 0.05). There were no differences for the drop protocol (p < 0.05). Rigid forks reduced overall exposure (p < 0.05), specifically at the handlebars for the stairs and drop trials. More detailed analysis presented smaller vertical accelerations at the handlebar for air-sprung and leaf-sprung forks on the stairs (p < 0.05), and drop (p < 0.05) but not the root. As such, it appears that the suspension systems tested were ineffective at reducing overall impact exposure at the handlebar during isolated aspects of cross-country terrain features which may be influenced to a larger extent by rider technique.

  4. Human ribonuclease H1 resolves R-loops and thereby enables progression of the DNA replication fork.

    Science.gov (United States)

    Parajuli, Shankar; Teasley, Daniel C; Murali, Bhavna; Jackson, Jessica; Vindigni, Alessandro; Stewart, Sheila A

    2017-09-15

    Faithful DNA replication is essential for genome stability. To ensure accurate replication, numerous complex and redundant replication and repair mechanisms function in tandem with the core replication proteins to ensure DNA replication continues even when replication challenges are present that could impede progression of the replication fork. A unique topological challenge to the replication machinery is posed by RNA-DNA hybrids, commonly referred to as R-loops. Although R-loops play important roles in gene expression and recombination at immunoglobulin sites, their persistence is thought to interfere with DNA replication by slowing or impeding replication fork progression. Therefore, it is of interest to identify DNA-associated enzymes that help resolve replication-impeding R-loops. Here, using DNA fiber analysis, we demonstrate that human ribonuclease H1 (RNH1) plays an important role in replication fork movement in the mammalian nucleus by resolving R-loops. We found that RNH1 depletion results in accumulation of RNA-DNA hybrids, slowing of replication forks, and increased DNA damage. Our data uncovered a role for RNH1 in global DNA replication in the mammalian nucleus. Because accumulation of RNA-DNA hybrids is linked to various human cancers and neurodegenerative disorders, our study raises the possibility that replication fork progression might be impeded, adding to increased genomic instability and contributing to disease. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  5. The Late Cretaceous Middle Fork caldera, its resurgent intrusion, and enduring landscape stability in east-central Alaska

    Science.gov (United States)

    Bacon, Charles R.; Dusel-Bacon, Cynthia; Aleinikoff, John N.; Slack, John F.

    2014-01-01

    Dissected caldera structures expose thick intracaldera tuff and, uncommonly, cogenetic shallow plutons, while remnants of correlative outflow tuffs deposited on the pre-eruption ground surface record elements of ancient landscapes. The Middle Fork caldera encompasses a 10 km × 20 km area of rhyolite welded tuff and granite porphyry in east-central Alaska, ∼100 km west of the Yukon border. Intracaldera tuff is at least 850 m thick. The K-feldspar megacrystic granite porphyry is exposed over much of a 7 km × 12 km area having 650 m of relief within the western part of the caldera fill. Sensitive high-resolution ion microprobe with reverse geometry (SHRIMP-RG) analyses of zircon from intracaldera tuff, granite porphyry, and outflow tuff yield U-Pb ages of 70.0 ± 1.2, 69.7 ± 1.2, and 71.1 ± 0.5 Ma (95% confidence), respectively. An aeromagnetic survey indicates that the tuff is reversely magnetized, and, therefore, that the caldera-forming eruption occurred in the C31r geomagnetic polarity chron. The tuff and porphyry have arc geochemical signatures and a limited range in SiO2 of 69 to 72 wt%. Although their phenocrysts differ in size and abundance, similar quartz + K-feldspar + plagioclase + biotite mineralogy, whole-rock geochemistry, and analytically indistinguishable ages indicate that the tuff and porphyry were comagmatic. Resorption of phenocrysts in tuff and porphyry suggests that these magmas formed by thermal rejuvenation of near-solidus or solidified crystal mush. A rare magmatic enclave (54% SiO2, arc geochemical signature) in the porphyry may be similar to parental magma and provides evidence of mafic magma and thermal input.

  6. Mercury and other Mining-Related Contaminants in Ospreys along the Upper Clark Fork River, MT

    Science.gov (United States)

    Langner, H.; Domenech, R.; Greene, E.; Staats, M. F.

    2010-12-01

    Osprey (Pandion haliaetus) are widely recognized as bio-indicators of the health of aquatic ecosystems. Until the time of fledging, nestlings feed exclusively on fish caught within a few kilometers of the nest. Therefore, tissues of these young birds may reflect the level of contamination of local fish and more generally, the contamination status of the aquatic ecosystem they inhabit. Nests can often be accessed with a boom truck and obtaining small blood samples from the flightless chicks is fairly noninvasive. Ospreys are nesting along the Upper Clark Fork River, Montana, which is heavily contaminated with wastes left from a century of copper and precious metals mining. We have been monitoring the levels of priority pollutants (arsenic, cadmium, lead, copper, zinc, mercury and selenium) in Osprey chicks along a 250 km section of the river for four years. Objectives are to establish current contaminant status, pinpoint pollution hotspots, and assess the success of restoration efforts. Our results suggest that of highest concern may be the bioaccumulation of mercury with blood levels of up to 0.7 mg/L in the growing chicks. These concentrations are expected to increase many fold upon fledging as feather growth stops, which acts as the major sink for mercury. Interestingly, we found mercury levels increased in downstream direction, in contrast to concentrations of other pollutants. Reasons may be the different origin of mercury versus other contaminants and the distribution of wetlands where mercury can be transformed into highly bioavailable methylmercury. Blood levels of selenium are also elevated throughout the Upper Clark Fork River drainage. We discuss the implications for restoration and remediation of the Clark Fork River.

  7. Dynamic behavior of tuning fork shear-force structures in a SNOM system

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Fengli [Department of Engineering Mechanics, AML, CNMM, Tsinghua University, Beijing 100084 (China); Li, Xide, E-mail: lixide@tsinghua.edu.cn [Department of Engineering Mechanics, AML, CNMM, Tsinghua University, Beijing 100084 (China); Wang, Jia [State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing 100084 (China); Fu, Yu [Temasek Laboratories, Nanyang Technological University, 50 Nanyang Drive, 637553 (Singapore)

    2014-07-01

    Piezoelectric tuning fork shear-force structures are widely used as a distance control unit in a scanning near-field optical microscopy. However, the complex dynamic behavior among the micro-tuning forks (TFs), optical fiber probes, and the probe–surface interactions is still a crucial issue to achieve high-resolution imaging or near-field interaction inspections. Based on nonlinear beam tension-bending vibration theory, vibration equations in both longitudinal and lateral directions have been established when the TF structure and the optical fiber are treated as deformable structures. The relationship of the probe–surface interaction induced by Van der Waals force has been analyzed and the corresponding numerical results used to describe the vibrational behavior of the probe approaching the sample surface are obtained. Meanwhile, the viscous resistance of the liquid film on the sample surface has also been investigated using linear beam-bending vibration theory. Experiments testing the interaction between the probe and the water film on a single crystal silicon wafer have been carried out and the viscous resistance of the water film was estimated using the established equations. Finally, to use the TF-probe structure as a force sensor, the relation between the dynamic response of the TF-probe system and an external force on the probe tip was obtained. - Highlights: • Nonlinear vibration equation is established for a deformable tuning fork probe assembly. • Probe–sample interactions induced by Van der Waals force and viscous resistance are investigated. • The viscous resistance between the probe and the water film is estimated using testing results.

  8. Geomorphic Change Induced by 100 years of Flow Alteration on the Diamond Fork River, Central Utah

    Science.gov (United States)

    Jones, J.; Belmont, P.; Wilcock, P. R.

    2017-12-01

    Changes in hydrology and sediment supply affect the form of rivers. The rate of change of fluvial form is controlled by a variety of factors, including valley confinement, sediment size, and antecedent condition. The Diamond Fork River in central Utah has been altered by trans-basin flows delivered from the Colorado River system for over a century. Beginning in 1915, water used for irrigation was delivered through a tributary, Sixth Water Creek, with daily summer flows regularly exceeding the 50 - 100 year flood. Elevated flows caused drastic geomorphic change - resulting in incision and widening of the channel, and the destruction of riparian vegetation. Beginning in 1997, the outlet for the trans-basin diversion was moved downstream on Sixth Water, bypassing a large landslide, and flows were drastically reduced in 2004 through management actions. We delineated eight distinct process domains for the Sixth Water-Diamond Fork system and examined the response of each process domain to the altered flow and sediment regimes through the analysis of aerial photographs and repeat cross-sections. We measured a variety of channel metrics, including channel width, areal extent of bars and islands, and sinuosity in ArcGIS. Results indicate that unconfined reaches that were wide and braided during the period of elevated flows have narrowed to become single threaded and meandering in response to the reduced flows. Confined reaches have experienced minor changes since the reduction in flows, suggesting that confinement is a primary control on the degree of channel response. These findings and complimentary studies will provide managers of Sixth Water and Diamond Fork with a greater understanding of the physical response of the streams, and the resulting effects on ecological communities.

  9. In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

    International Nuclear Information System (INIS)

    Gauld, Ian C.; Hu, Jianwei; De Baere, P.; Tobin, Stephen

    2015-01-01

    Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the framework of the US Department of Energy-EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel

  10. In-Field Performance Testing of the Fork Detector for Quantitative Spent Fuel Verification

    Energy Technology Data Exchange (ETDEWEB)

    Gauld, Ian C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hu, Jianwei [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); De Baere, P. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Vaccaro, S. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Schwalbach, P. [European Commission (Luxembourg). DG Energy, Directorate Nuclear Safeguards; Liljenfeldt, Henrik [Swedish Nuclear Fuel and Waste Management Company (Sweden); Tobin, Stephen [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-01-01

    Expanding spent fuel dry storage activities worldwide are increasing demands on safeguards authorities that perform inspections. The European Atomic Energy Community (EURATOM) and the International Atomic Energy Agency (IAEA) require measurements to verify declarations when spent fuel is transferred to difficult-to-access locations, such as dry storage casks and the repositories planned in Finland and Sweden. EURATOM makes routine use of the Fork detector to obtain gross gamma and total neutron measurements during spent fuel inspections. Data analysis is performed by modules in the integrated Review and Analysis Program (iRAP) software, developed jointly by EURATOM and the IAEA. Under the framework of the US Department of Energy–EURATOM cooperation agreement, a module for automated Fork detector data analysis has been developed by Oak Ridge National Laboratory (ORNL) using the ORIGEN code from the SCALE code system and implemented in iRAP. EURATOM and ORNL recently performed measurements on 30 spent fuel assemblies at the Swedish Central Interim Storage Facility for Spent Nuclear Fuel (Clab), operated by the Swedish Nuclear Fuel and Waste Management Company (SKB). The measured assemblies represent a broad range of fuel characteristics. Neutron count rates for 15 measured pressurized water reactor assemblies are predicted with an average relative standard deviation of 4.6%, and gamma signals are predicted on average within 2.6% of the measurement. The 15 measured boiling water reactor assemblies exhibit slightly larger deviations of 5.2% for the gamma signals and 5.7% for the neutron count rates, compared to measurements. These findings suggest that with improved analysis of the measurement data, existing instruments can provide increased verification of operator declarations of the spent fuel and thereby also provide greater ability to confirm integrity of an assembly. These results support the application of the Fork detector as a fully quantitative spent fuel

  11. Performance Characteristics and Temperature Compensation Method of Fluid Property Sensor Based on Tuning-Fork Technology

    Directory of Open Access Journals (Sweden)

    Yuan Chen

    2016-01-01

    Full Text Available Fluid property sensor (FPS based on tuning-fork technology is applied to the measurement of the contaminant level of lubricant oil. The measuring principle of FPS sensor is derived and proved together with its resolution. The performance characteristics of the FPS sensor, such as sensitivity coefficient, resolution, and quality factor, are analyzed. A temperature compensation method is proposed to eliminate the temperature-dependence of the measuring parameters, and its validity is investigated by numerical simulation of sensitivity, oscillating frequency, and dielectric constant. The values of purification efficiency obtained using microwave and without microwave are compared experimentally.

  12. Evaluation of Lower East Fork Poplar Creek Mercury Sources - Model Update

    Energy Technology Data Exchange (ETDEWEB)

    Ketelle, Richard [East Tennessee Technology Park (ETTP), Oak Ridge, TN (United States); Brandt, Craig C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Bevelhimer, Mark S. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2017-08-01

    The purpose of this report is to assess new data that has become available and provide an update to the evaluations and modeling presented in the Oak Ridge National Laboratory (ORNL) Technical Manuscript Evaluation of lower East Fork Poplar Creek (LEFPC) Mercury Sources (Watson et al., 2016). Primary sources of field and laboratory data for this update include multiple US Department of Energy (DOE) programs including Environmental Management (EM; e.g., Biological Monitoring and Abatement Program, Mercury Remediation Technology Development [TD], and Applied Field Research Initiative), Office of Science (Mercury Science Focus Areas [SFA] project), and the Y-12 National Security Complex (Y-12) Compliance Department.

  13. Best management practices plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This plan was prepared in support of the Phase II Remedial Design Report (DOE/OR/01-1449 ampersand D1) and in accordance with requirements under CERCLA to present the plan for best management practices to be followed during the remediation. This document provides the Environmental Restoration Program with information about spill prevention and control, water quality monitoring, good housekeeping practices, sediment and erosion control measures, and inspections and environmental compliance practices to be used during Phase II of the remediation project for the Lower East Fork Poplar Creek Operable Unit

  14. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2016 Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Dickson, Johnbull O. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Smith, John G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mehlhorn, Tonia L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Lowe, Kenneth Alan [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Morris, Jesse G. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Johs, Alexander [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); McManamay, Ryan A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Poteat, Monica D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Olsen, Todd A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Eller, Virginia A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Gonez Rodriguez, Leroy [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)

    2017-07-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM), especially at and near the Y-12 National Security Complex (Y-12) where historical mercury use has resulted in contaminated buildings, soils, and downstream surface waters. To address mercury contamination of East Fork Poplar Creek (EFPC), the DOE has adopted a phased, adaptive management approach to remediation, which includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014).

  15. Distance control for a near-field scanning microwave microscope in liquid using a quartz tuning fork

    International Nuclear Information System (INIS)

    Kim, Song Hul; Yoo, Hyun Jun; Yoo, Hyung Geun; Lee, Kie Jin

    2004-01-01

    We demonstrate a scanning near-field microwave microscope (NSMM) in the liquid environment using a tuning fork shear-force feedback method to control the distance between tip and sample. The probe tip for the NSMM is only immersed in water and attached to one prong of a quartz tuning fork and directly coupled to a high-quality dielectric resonator at an operating frequency f = 4.5-5.5 GHz. This distance control method is independent of the local microwave characteristics. The amplitude of the tuning fork was used as a set point of the distance control parameter in the liquid. To demonstrate the ability of the distance regulation system, we present the NSMM images of a copper film in air and liquid without and with readjusting the distance set point and a DNA film image in buffer solution.

  16. Replication stress-induced chromosome breakage is correlated with replication fork progression and is preceded by single-stranded DNA formation.

    Science.gov (United States)

    Feng, Wenyi; Di Rienzi, Sara C; Raghuraman, M K; Brewer, Bonita J

    2011-10-01

    Chromosome breakage as a result of replication stress has been hypothesized to be the direct consequence of defective replication fork progression, or "collapsed" replication forks. However, direct and genome-wide evidence that collapsed replication forks give rise to chromosome breakage is still lacking. Previously we showed that a yeast replication checkpoint mutant mec1-1, after transient exposure to replication impediment imposed by hydroxyurea (HU), failed to complete DNA replication, accumulated single-stranded DNA (ssDNA) at the replication forks, and fragmented its chromosomes. In this study, by following replication fork progression genome-wide via ssDNA detection and by direct mapping of chromosome breakage after HU exposure, we have tested the hypothesis that the chromosome breakage in mec1 cells occurs at collapsed replication forks. We demonstrate that sites of chromosome breakage indeed correlate with replication fork locations. Moreover, ssDNA can be detected prior to chromosome breakage, suggesting that ssDNA accumulation is the common precursor to double strand breaks at collapsed replication forks.

  17. Qualitative Assessment: Evaluating the Impacts of Climate Change on Endangered Species Act Recovery Actions for the South Fork Nooksack River, WA

    Science.gov (United States)

    The South Fork Nooksack River (South Fork) is located in northwest Washington State and is home to nine species of Pacific salmon, including Nooksack early Chinook (aka, spring Chinook salmon), an iconic species for the Nooksack Indian Tribe. The quantity of salmon in the South F...

  18. Atomic resolution ultrafast scanning tunneling microscope with scan rate breaking the resonant frequency of a quartz tuning fork resonator.

    Science.gov (United States)

    Li, Quanfeng; Lu, Qingyou

    2011-05-01

    We present an ultra-fast scanning tunneling microscope with atomic resolution at 26 kHz scan rate which surpasses the resonant frequency of the quartz tuning fork resonator used as the fast scan actuator. The main improvements employed in achieving this new record are (1) fully low voltage design (2) independent scan control and data acquisition, where the tuning fork (carrying a tip) is blindly driven to scan by a function generator with the scan voltage and tunneling current (I(T)) being measured as image data (this is unlike the traditional point-by-point move and measure method where data acquisition and scan control are switched many times).

  19. The Model Analysis of a Complex Tuning Fork Probe and Its Application in Bimodal Atomic Force Microscopy

    Directory of Open Access Journals (Sweden)

    Zhichao Wu

    2017-01-01

    Full Text Available A new electromechanical coupling model was built to quantitatively analyze the tuning fork probes, especially the complex ones. A special feature of a novel, soft tuning fork probe, that the second eigenfrequency of the probe was insensitive to the effective force gradient, was found and used in a homemade bimodal atomic force microscopy to measure power dissipation quantitatively. By transforming the mechanical parameters to the electrical parameters, a monotonous and concise method without using phase to calculate the power dissipation was proposed.

  20. Lentiform fork sign: a magnetic resonance finding in a case of acute metabolic acidosis.

    Science.gov (United States)

    Grasso, Daniela; Borreggine, Carmela; Perfetto, Francesco; Bertozzi, Vincenzo; Trivisano, Marina; Specchio, Luigi Maria; Grilli, Gianpaolo; Macarini, Luca

    2014-06-01

    We report a 33 year-old woman addicted to chronic unspecified solvents abuse with stupor, respiratory disorders, tetraplegia and severe metabolic acidosis. On admission an unenhanced cranial CT scan showed symmetrical hypodensities of both lentiform nuclei. MR imaging performed 12 hours after stupor demonstrates bilateral putaminal hemorrhagic necrosis, bilateral external capsule, corona radiata and deep cerebellar hyperintensities with right cingulate cortex involvement. DWI reflected bilateral putaminal hyperintensities with restricted water diffusion as to citotoxic edema and development of vasogenic edema in the external capsule recalling a fork. On day twenty, after specific treatments MRI demonstrated a bilateral putaminal marginal enhancement. Bilateral putaminal necrosis is a characteristic but non-specific radiological finding of methanol poisoning. Lentiform Fork sign is a rare MRI finding reported in literature in 22 patients with various conditions characterized by metabolic acidosis. Vasogenic edema may be due to the differences in metabolic vulnerability between neurons and astrocytes. We postulate that metabolic acidosis could have an important role to generate this sign.

  1. Molecular basis for PrimPol recruitment to replication forks by RPA.

    Science.gov (United States)

    Guilliam, Thomas A; Brissett, Nigel C; Ehlinger, Aaron; Keen, Benjamin A; Kolesar, Peter; Taylor, Elaine M; Bailey, Laura J; Lindsay, Howard D; Chazin, Walter J; Doherty, Aidan J

    2017-05-23

    DNA damage and secondary structures can stall the replication machinery. Cells possess numerous tolerance mechanisms to complete genome duplication in the presence of such impediments. In addition to translesion synthesis (TLS) polymerases, most eukaryotic cells contain a multifunctional replicative enzyme called primase-polymerase (PrimPol) that is capable of directly bypassing DNA damage by TLS, as well as repriming replication downstream of impediments. Here, we report that PrimPol is recruited to reprime through its interaction with RPA. Using biophysical and crystallographic approaches, we identify that PrimPol possesses two RPA-binding motifs and ascertained the key residues required for these interactions. We demonstrate that one of these motifs is critical for PrimPol's recruitment to stalled replication forks in vivo. In addition, biochemical analysis reveals that RPA serves to stimulate the primase activity of PrimPol. Together, these findings provide significant molecular insights into PrimPol's mode of recruitment to stalled forks to facilitate repriming and restart.

  2. Dynamic force microscopy with quartz tuning forks at high oscillation amplitudes

    International Nuclear Information System (INIS)

    Labardi, M

    2007-01-01

    Dynamic force microscopy (DFM) with the self-oscillator (SO) method allows reasonably high scanning rates even with high Q-factors of the resonant force sensor, typical of cantilevers in ultra-high vacuum and of quartz tuning forks. However, due to simpler interpretation of force spectroscopy measurements, small oscillation amplitudes (sub-nm level) are generally preferred. In applications like 'apertureless' scanning near-field optical microscopy (SNOM), oscillation amplitudes of the order of 5-10 nm are needed to increase optical sensitivity and to apply standard optical artefact suppression methods. This motivates the study of the behaviour of tuning forks driven at such high amplitudes, as compared to usual air-operated cantilevers. Both constant-excitation-amplitude (CE) and constant-oscillation-amplitude (CA) modes of SO-DFM are analysed, since the CA mode is more convenient for SNOM applications, denoting remarkable differences. In particular, possible instability effects, previously found in CE mode, are not anticipated for CA mode. It is shown how resonance and approach ('isophase') curves in both modes can be conveniently described in terms of the usual 'normalized frequency shift' γ and of a 'normalized gain' η, defined as a measurement of surface dissipation

  3. Spatial Patterns of Mercury Bioaccumulation in the Upper Clark Fork River Basin, MT

    Science.gov (United States)

    Staats, M. F.; Langner, H.; Moore, J. N.

    2010-12-01

    The Upper Clark Fork River Basin (UCFRB) in Montana has a legacy of historic gold/silver mine waste that contributes large quantities of mercury into the watershed. Mercury bioaccumulation at higher levels of the aquatic food chain, such as the mercury concentration in the blood of pre-fledge osprey, exhibit an irregular spatial signature based on the location of the nests throughout the river basin. Here we identify regions with a high concentration of bioavailable mercury and the major factors that allow the mercury to bioaccumulate within trophic levels. This identification is based on the abundance of mercury sources and the potential for mercury methylation. To address the source term, we did a survey of total mercury in fine sediments along selected UCFRB reaches, along with the assessment of environmental river conditions (percentage of backwaters/wetlands, water temperature and pH, etc). In addition, we analyzed the mercury levels of a representative number of macroinvertebrates and fish from key locations. The concentration of total mercury in sediment, which varies from reach to reach (tributaries of the Clark Fork River, 5mg/kg) affects the concentration of mercury found at various trophic levels. However, reaches with a low supply of mine waste-derived mercury can also yield substantial concentrations of mercury in the biota, due to highly favorable conditions for mercury methylation. We identify that the major environmental factor that affects the methylation potential in the UCFRB is the proximity and connectivity of wetland areas to the river.

  4. The ammonoids from the Three Forks Shale (Late Devonian of Montana

    Directory of Open Access Journals (Sweden)

    D. Korn

    2006-08-01

    Full Text Available The ammonoid fauna from the Late Devonian Three Forks Shale of Montana is revised. Six taxa were recognised, which belong to the genera Tornoceras, Pernoceras, Raymondiceras, Platyclymenia, Pleuroclymenia, and Carinoclymenia. The ammonoid assemblage suggests a stratigraphic position within the middle Famennian, most probably the Platyclymenia annulata Zone. The ammonoids display extreme septal crowding in intermediate as well as adult growth stages, which can be regarded as evidence for instable palaeoecological conditions during lifetime of the animals. Die Ammonoideenfauna aus dem oberdevonischen Three Forks Shale von Montana wird revidiert. Sechs Taxa werden unterschieden; sie gehören zu den Gattungen Tornoceras, Pernoceras, Raymondiceras, Platyclymenia, Pleuroclymenia und Carinoclymenia. Die Ammonoideen-Vergesellschaftung spricht für eine stratigraphische Position im mittleren Famennium, wahrscheinlich in der Platyclymenia annulata Zone. Die Ammonoideen zeigen auffällige Drängung der Septen in intermediären und adulten Wachstumsstadien, die als Hinweis auf instabile Lebensbedingungen für die Tiere gewertet werden kann. doi:10.1002/mmng.200600008

  5. Correlates of mercury in fish from lakes near Clyde Forks, Ontario, Canada

    International Nuclear Information System (INIS)

    Ethier, A.L.M.; Scheuhammer, A.M.; Bond, D.E.

    2008-01-01

    Subsurface soils near Clyde Forks, Ontario, Canada, can have naturally high concentrations of mercury (Hg) from local geological sources. To investigate Hg in local aquatic food webs, Hg was measured in fish dorsal muscle (mainly yellow perch [YP] and pumpkinseed sunfish [PS]) and surface sediments from 10 regional lakes. Water chemistry, along with fork length, weight, and stable isotopes (δ 15 N, δ 13 C, δ 34 S) in fish were also measured. No lake sediments had elevated (>0.3 μg/g dw) Hg, and average Hg concentrations in fish were not sufficiently high ( 13 C), and certain lake variables (e.g., pH for YP). PS with more pelagic feeding habits had higher δ 34 S and Hg than those with more littoral feeding habits. Potential biological linkages between fish Hg and δ 34 S, a parameter that may be related to the lake sulphate-reducing bacteria activity, requires further investigation. - Fish from lakes near a localized geological Hg source do not have elevated Hg concentrations

  6. Investigation of shallow groundwater contamination near East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Carmichael, J.K.

    1989-01-01

    Alluvial soils of the flood plain of East Fork Poplar Creek in Oak Ridge, Tennessee, are contaminated with mercury and other metals, organic compounds, and radionuclides originating from the Y-12 Plant, a nuclear-processing facility located within the US Department of Energy's Oak Ridge Reservation. Observation wells were installed in the shallow aquifer of the flood plain, and water quality samples were collected to determine if contaminants are present in the shallow groundwater. Groundwater in the shallow aquifer occurs under water-table conditions. Recharge is primarily from precipitation and discharge is to East Fork Poplar Creek. Groundwater levels fluctuate seasonally in response to variations in recharge and evapotranspiration. During extremely dry periods, the water table drops below the base of the shallow aquifer in some flood-plain areas. Contaminants were found in water samples from several of the wells in concentrations which equaled or exceeded drinking-water standards established by the US Environmental Protection Agency are antimony, chromium, lead, mercury, selenium, phenols, and strontium-90. Total and dissolved uranium concentrations exceeded the analytical detection limit in nearly 70% of the wells in the flood plain. The results of water quality determinations demonstrate that elevated concentrations of most trace metals (and possibly organic compounds and radionuclides) were caused by contaminated sediments in the samples. The presence of contaminated sediment in samples is suspected to be the result of borehole contamination during well installation. 21 refs., 20 figs., 6 tabs

  7. Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.

    Science.gov (United States)

    Kurat, Christoph F; Yeeles, Joseph T P; Patel, Harshil; Early, Anne; Diffley, John F X

    2017-01-05

    The integrity of eukaryotic genomes requires rapid and regulated chromatin replication. How this is accomplished is still poorly understood. Using purified yeast replication proteins and fully chromatinized templates, we have reconstituted this process in vitro. We show that chromatin enforces DNA replication origin specificity by preventing non-specific MCM helicase loading. Helicase activation occurs efficiently in the context of chromatin, but subsequent replisome progression requires the histone chaperone FACT (facilitates chromatin transcription). The FACT-associated Nhp6 protein, the nucleosome remodelers INO80 or ISW1A, and the lysine acetyltransferases Gcn5 and Esa1 each contribute separately to maximum DNA synthesis rates. Chromatin promotes the regular priming of lagging-strand DNA synthesis by facilitating DNA polymerase α function at replication forks. Finally, nucleosomes disrupted during replication are efficiently re-assembled into regular arrays on nascent DNA. Our work defines the minimum requirements for chromatin replication in vitro and shows how multiple chromatin factors might modulate replication fork rates in vivo. Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.

  8. Hydrologic modeling of flood conveyance and impacts of historic overbank sedimentation on West Fork Black's Fork, Uinta Mountains, northeastern Utah, USA

    Science.gov (United States)

    Carson, Eric C.

    2006-05-01

    This study assesses historic overbank alluvial sedimentation along a low-gradient reach of West Fork Black's Fork in the northern Uinta Mountains, Utah. In this previously glaciated setting, an alluvial floodplain that is approximately 400 m wide by 1500 m long has been modified by the combined effects of valley morphometry and the recent history of clear-cut logging during the late 19th and early 20th Centuries. To quantify the effects on sedimentation and flow conveyance, three natural streambank exposures were sampled and analyzed for nuclear bomb fallout 137Cs. The distribution of 137Cs within the three profiles suggests that a remnant outwash terrace exerts a first-order control over the deposition of overbank alluvium. Upstream from a constriction in the floodplain caused by the terrace remnant, as much as 40 cm of overbank alluvium has been deposited since the beginning of clear-cut logging. Immediately downstream of that constriction, no evidence exists for any overbank sedimentation during that same period. Vibracore samples and Oakfield soil probe sampling throughout the study reach quantified the geographic extent and thicknesses of the historic alluvial package. Flood conveyance through the study area was modeled using the U.S. Army Corps of Engineers HEC-RAS modeling program. Model simulations were run for modern conditions (using surveyed topography) and for prehistoric conditions (using the modern topography less the historic alluvial package determined by 137Cs analyses). Model results indicate that the floodplain constriction caused a significant impediment to flood conveyance at even modest discharges during prehistoric conditions. This promoted ponding of floodwaters upstream of the constriction and deposition of alluvium. This has increased bank heights upstream of the constriction, to the point that under modern conditions 1- to 5-year recurrence interval floods are largely confined within the channel. These results confirm the validity of this

  9. Fork2Farmer: Enabling Success of Small Farms through Partnerships with Well-Known Chefs and the Tourism Sector

    Science.gov (United States)

    Morais, Duarte; Jakes, Susan; Bowen, Becky; Lelekacs, Joanna Massey

    2017-01-01

    A team of economic development, local foods, and tourism specialists from North Carolina Cooperative Extension is pursuing an initiative titled Fork2Farmer. The goal is to increase visits to local farms and diversify farm income by leveraging the high visibility of well-known farm-to-table chefs who support local small farms. To do this, those…

  10. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair

    Directory of Open Access Journals (Sweden)

    Zeina Kais

    2016-06-01

    Full Text Available BRCA1/2 proteins function in homologous recombination (HR-mediated DNA repair and cooperate with Fanconi anemia (FA proteins to maintain genomic integrity through replication fork stabilization. Loss of BRCA1/2 proteins results in DNA repair deficiency and replicative stress, leading to genomic instability and enhanced sensitivity to DNA-damaging agents. Recent studies have shown that BRCA1/2-deficient tumors upregulate Polθ-mediated alternative end-joining (alt-EJ repair as a survival mechanism. Whether other mechanisms maintain genomic integrity upon loss of BRCA1/2 proteins is currently unknown. Here we show that BRCA1/2-deficient tumors also upregulate FANCD2 activity. FANCD2 is required for fork protection and fork restart in BRCA1/2-deficient tumors. Moreover, FANCD2 promotes Polθ recruitment at sites of damage and alt-EJ repair. Finally, loss of FANCD2 in BRCA1/2-deficient tumors enhances cell death. These results reveal a synthetic lethal relationship between FANCD2 and BRCA1/2, and they identify FANCD2 as a central player orchestrating DNA repair pathway choice at the replication fork.

  11. The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

    DEFF Research Database (Denmark)

    Larsen, Nicolai B; Sass, Ehud; Suski, Catherine

    2014-01-01

    Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus...... as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications....

  12. Computer Aided Design of the Link-Fork Head-Piston Assembly of the Kaplan Turbine with Solidworks

    Directory of Open Access Journals (Sweden)

    Camelia Jianu

    2010-10-01

    Full Text Available The paper presents the steps for 3D computer aided design (CAD of the link-fork head-piston assembly of the Kaplan turbine made in SolidWorks.The present paper is a tutorial for a Kaplan turbine assembly 3D geometry, which is dedicated to the Assembly design and Drawing Geometry and Drawing Annotation.

  13. MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context

    DEFF Research Database (Denmark)

    Bentsen, Iben Bach; Nielsen, Ida; Lisby, Michael

    2013-01-01

    location within the rDNA. We discover a pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at RFBs, which differs from its acknowledged function in double-strand break processing. Consequently, in the absence of the MRX complex, single-stranded DNA (ssDNA) accumulates at the r...

  14. Reliability and responsiveness of a graduated tuning fork in immune mediated polyneuropathies. The Inflammatory Neuropathy Cause and Treatment (INCAT) Group

    NARCIS (Netherlands)

    I.S.J. Merkies (Ingemar); P.I.M. Schmitz (Paul); F.G.A. van der Meché (Frans); P.A. van Doorn (Pieter)

    2000-01-01

    textabstractThe interobserver and intraobserver reliability of the Rydel-Seiffer (RS) graduated tuning fork was evaluated in 113 patients with a clinically stable immune mediated polyneuropathy (83 patients who had had Guillain-Barre syndrome (GBS) in the past, 22 with

  15. The effect of real-time vibrotactile feedback delivered through an augmented fork on eating rate, satiation, and food intake

    NARCIS (Netherlands)

    Roel C. J. Hermans; Sander Hermsen; Eric Robinson; Suzanne Higgs; Jeana H. Frost; Monica Mars

    2017-01-01

    Eating rate is a basic determinant of appetite regulation, as people who eat more slowly feel sated earlier and eat less. Without assistance, eating rate is difficult to modify due to its automatic nature. In the current study, participants used an augmented fork that aimed to decelerate their rate

  16. Mapping Prehistoric, Historic, and Channel Sediment Distribution, South Fork Noyo River: A Tool For Understanding Sources, Storage, and Transport

    Science.gov (United States)

    Rich D. Koehler; Keith I. Kelson; Graham Matthews; K.H. Kang; Andrew D. Barron

    2007-01-01

    The South Fork Noyo River (SFNR) watershed in coastal northern California contains large volumes of historic sediment that were delivered to channels in response to past logging operations. This sediment presently is stored beneath historic terraces and in present-day channels. We conducted geomorphic mapping on the SFNR valley floor to assess the volume and location...

  17. 75 FR 41237 - Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the American River; California

    Science.gov (United States)

    2010-07-15

    ... DEPARTMENT OF THE INTERIOR Bureau of Land Management [LLCAC08000-L1430000-ET0000; CACA 41334] Public Land Order No. 7746; Withdrawal of Public Lands, South Fork of the American River; California AGENCY: Bureau of Land Management, Interior. ACTION: Public Land Order. SUMMARY: This order withdraws 2...

  18. Fork-join and data-driven execution models on multi-core architectures: Case study of the FMM

    KAUST Repository

    Amer, Abdelhalim; Maruyama, Naoya; Pericà s, Miquel; Taura, Kenjiro; Yokota, Rio; Matsuoka, Satoshi

    2013-01-01

    Extracting maximum performance of multi-core architectures is a difficult task primarily due to bandwidth limitations of the memory subsystem and its complex hierarchy. In this work, we study the implications of fork-join and data-driven execution

  19. Hydrologic and hydraulic analyses for the Black Fork Mohican River Basin in and near Shelby, Ohio

    Science.gov (United States)

    Huitger, Carrie A.; Ostheimer, Chad J.; Koltun, G.F.

    2016-05-06

    Hydrologic and hydraulic analyses were done for selected reaches of five streams in and near Shelby, Richland County, Ohio. The U.S. Geological Survey (USGS), in cooperation with the Muskingum Watershed Conservancy District, conducted these analyses on the Black Fork Mohican River and four tributaries: Seltzer Park Creek, Seltzer Park Tributary, Tuby Run, and West Branch. Drainage areas of the four stream reaches studied range from 0.51 to 60.3 square miles. The analyses included estimation of the 10-, 2-, 1-, and 0.2-percent annual-exceedance probability (AEP) flood-peak discharges using the USGS Ohio StreamStats application. Peak discharge estimates, along with cross-sectional and hydraulic structure geometries, and estimates of channel roughness coefficients were used as input to step-backwater models. The step-backwater water models were used to determine water-surface elevation profiles of four flood-peak discharges and a regulatory floodway. This study involved the installation of, and data collection at, a streamflow-gaging station (Black Fork Mohican River at Shelby, Ohio, 03129197), precipitation gage (Rain gage at Reservoir Number Two at Shelby, Ohio, 405209082393200), and seven submersible pressure transducers on six selected river reaches. Two precipitation-runoff models, one for the winter events and one for nonwinter events for the headwaters of the Black Fork Mohican River, were developed and calibrated using the data collected. With the exception of the runoff curve numbers, all other parameters used in the two precipitation-runoff models were identical. The Nash-Sutcliffe model efficiency coefficients were 0.737, 0.899, and 0.544 for the nonwinter events and 0.850 and 0.671 for the winter events. Both of the precipitation-runoff models underestimated the total volume of water, with residual runoff ranging from -0.27 inches to -1.53 inches. The results of this study can be used to assess possible mitigation options and define flood hazard areas that

  20. Flood-inundation maps for North Fork Salt Creek at Nashville, Indiana

    Science.gov (United States)

    Martin, Zachary W.

    2017-11-13

    Digital flood-inundation maps for a 3.2-mile reach of North Fork Salt Creek at Nashville, Indiana, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science website at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding that correspond to selected water levels (stages) at the North Fork Salt Creek at Nashville, Ind., streamgage (USGS station number 03371650). Real-time stages at this streamgage may be obtained from the USGS National Water Information System at http://waterdata.usgs.gov/nwis or the National Weather Service (NWS) Advanced Hydrologic Prediction Service at http:/water.weather.gov/ahps/, which also shows observed USGS stages at the same site as the USGS streamgage (NWS site NFSI3).Flood profiles were computed for the stream reach by means of a one-dimensional, step-backwater hydraulic modeling software developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated using the current (2015) stage-discharge rating at the USGS streamgage 03371650, North Fork Salt Creek at Nashville, Ind. The hydraulic model was then used to compute 12 water-surface profiles for flood stages at 1-foot (ft) intervals, except for the highest profile of 22.9 ft, referenced to the streamgage datum ranging from 12.0 ft (the NWS “action stage”) to 22.9 ft, which is the highest stage of the current (2015) USGS stage-discharge rating curve and 1.9 ft higher than the NWS “major flood stage.” The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from light detection and ranging data having a 0.98-ft vertical accuracy and 4.9-ft horizontal resolution) to delineate the area flooded at each stage.The availability of these maps, along with information regarding current stage from the USGS

  1. Frequency shift, damping, and tunneling current coupling with quartz tuning forks in noncontact atomic force microscopy

    Science.gov (United States)

    Nony, Laurent; Bocquet, Franck; Para, Franck; Loppacher, Christian

    2016-09-01

    A combined experimental and theoretical approach to the coupling between frequency-shift (Δ f ) , damping, and tunneling current (It) in combined noncontact atomic force microscopy/scanning tunneling microscopy using quartz tuning forks (QTF)-based probes is reported. When brought into oscillating tunneling conditions, the tip located at the QTF prong's end radiates an electromagnetic field which couples to the QTF prong motion via its piezoelectric tensor and loads its electrodes by induction. Our approach explains how those It-related effects ultimately modify the Δ f and the damping measurements. This paradigm to the origin of the coupling between It and the nc-AFM regular signals relies on both the intrinsic piezoelectric nature of the quartz constituting the QTF and its electrodes design.

  2. Piezoelectric tuning fork biosensors for the quantitative measurement of biomolecular interactions

    International Nuclear Information System (INIS)

    Gonzalez, Laura; Maria Benito, Angel; Puig-Vidal, Manel; Otero, Jorge; Rodrigues, Mafalda; Pérez-García, Lluïsa

    2015-01-01

    The quantitative measurement of biomolecular interactions is of great interest in molecular biology. Atomic force microscopy (AFM) has proved its capacity to act as a biosensor and determine the affinity between biomolecules of interest. Nevertheless, the detection scheme presents certain limitations when it comes to developing a compact biosensor. Recently, piezoelectric quartz tuning forks (QTFs) have been used as laser-free detection sensors for AFM. However, only a few studies along these lines have considered soft biological samples, and even fewer constitute quantified molecular recognition experiments. Here, we demonstrate the capacity of QTF probes to perform specific interaction measurements between biotin–streptavidin complexes in buffer solution. We propose in this paper a variant of dynamic force spectroscopy based on representing adhesion energies E (aJ) against pulling rates v (nm s"–"1). Our results are compared with conventional AFM measurements and show the great potential of these sensors in molecular interaction studies. (paper)

  3. Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.

    Science.gov (United States)

    Choe, Katherine N; Moldovan, George-Lucian

    2017-02-02

    Proliferating cell nuclear antigen (PCNA) lies at the center of the faithful duplication of eukaryotic genomes. With its distinctive doughnut-shaped molecular structure, PCNA was originally studied for its role in stimulating DNA polymerases. However, we now know that PCNA does much more than promote processive DNA synthesis. Because of the complexity of the events involved, cellular DNA replication poses major threats to genomic integrity. Whatever predicament lies ahead for the replication fork, PCNA is there to orchestrate the events necessary to handle it. Through its many protein interactions and various post-translational modifications, PCNA has far-reaching impacts on a myriad of cellular functions. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. [History of the tuning fork. II: Evolution of the classical experiments by Weber, Rinne and Schwabach].

    Science.gov (United States)

    Feldmann, H

    1997-05-01

    Since the 17th centrury it was known that sounds could be perceived via air conduction and bone conduction and that this provided a means of differentiating between hearing disorders located in the middle ear and those located in the acoustic nerve. For a long time to come, however, there was no need for such a differential diagnosis. After the invention of the tuning fork in 1711 this instrument had soon become widely used in music, but it took well over 100 years until it was introduced into physiology and otology. FROM DIRECTIONAL HEARING TO WEBER'S TEST: J. B. Venturi, a physicist in Modena, Italy, in 1802 had shown that the perception of the direction from which a sound is coming is governed by the fact that one ear is hit by the sound more intensely than the other ear. C. T. Tourtual, a physician in Münster, Germany, demonstrated in 1827 that this also holds true for sound conducted via the skull bones. He used a watch as sound source. He found that occlusion of both ear canals would increase the sensation in both ears equally, but that occlusion of only one ear would increase the sensation only in the occluded ear, thus giving the impression that the sound were coming from that side. He was interested in a comparison between vision and audition, and he concluded that with regard to recognizing the direction of a sensory signal vision was superior to audition. In the same year 1827 C. Wheatstone, a physicist in London, investigating the mode of vibration of the tympanic membrane and using a tuning fork found the same phenomena as Tourtual and some more effects. E. H. Weber, an anatomist and physiologist in Leipzig, Germany, described the very same phenomena as Tourtual and Wheatstone once more in 1834. He wanted to prove that airborne sound is perceived by the vestibulum and the semicircular canals, bone conducted sound by the cochlea. None of these investigators was thinking of a clinical use of their findings and made no such suggestion. E. Schmalz, an

  5. The phase interrogation method for optical fiber sensor by analyzing the fork interference pattern

    Science.gov (United States)

    Lv, Riqing; Qiu, Liqiang; Hu, Haifeng; Meng, Lu; Zhang, Yong

    2018-02-01

    The phase interrogation method for optical fiber sensor is proposed based on the fork interference pattern between the orbital angular momentum beam and plane wave. The variation of interference pattern with phase difference between the two light beams is investigated to realize the phase interrogation. By employing principal component analysis method, the features of the interference pattern can be extracted. Moreover, the experimental system is designed to verify the theoretical analysis, as well as feasibility of phase interrogation. In this work, the Mach-Zehnder interferometer was employed to convert the strain applied on sensing fiber to the phase difference between the reference and measuring paths. This interrogation method is also applicable for the measurements of other physical parameters, which can produce the phase delay in optical fiber. The performance of the system can be further improved by employing highlysensitive materials and fiber structures.

  6. Quartz enhanced photoacoustic spectroscopy based trace gas sensors using different quartz tuning forks.

    Science.gov (United States)

    Ma, Yufei; Yu, Guang; Zhang, Jingbo; Yu, Xin; Sun, Rui; Tittel, Frank K

    2015-03-27

    A sensitive trace gas sensor platform based on quartz-enhanced photoacoustic spectroscopy (QEPAS) is reported. A 1.395 μm continuous wave (CW), distributed feedback pigtailed diode laser was used as the excitation source and H2O was selected as the target analyte. Two kinds of quartz tuning forks (QTFs) with a resonant frequency (f0) of 30.72 kHz and 38 kHz were employed for the first time as an acoustic wave transducer, respectively for QEPAS instead of a standard QTF with a f0 of 32.768 kHz. The QEPAS sensor performance using the three different QTFs was experimentally investigated and theoretically analyzed. A minimum detection limit of 5.9 ppmv and 4.3 ppmv was achieved for f0 of 32.768 kHz and 30.72 kHz, respectively.

  7. Quartz Enhanced Photoacoustic Spectroscopy Based Trace Gas Sensors Using Different Quartz Tuning Forks

    Directory of Open Access Journals (Sweden)

    Yufei Ma

    2015-03-01

    Full Text Available A sensitive trace gas sensor platform based on quartz-enhanced photoacoustic spectroscopy (QEPAS is reported. A 1.395 μm continuous wave (CW, distributed feedback pigtailed diode laser was used as the excitation source and H2O was selected as the target analyte. Two kinds of quartz tuning forks (QTFs with a resonant frequency (f0 of 30.72 kHz and 38 kHz were employed for the first time as an acoustic wave transducer, respectively for QEPAS instead of a standard QTF with a f0 of 32.768 kHz. The QEPAS sensor performance using the three different QTFs was experimentally investigated and theoretically analyzed. A minimum detection limit of 5.9 ppmv and 4.3 ppmv was achieved for f0 of 32.768 kHz and 30.72 kHz, respectively.

  8. Modulation of mutagenesis in eukaryotes by DNA replication fork dynamics and quality of nucleotide pools

    Science.gov (United States)

    Waisertreiger, Irina S.-R.; Liston, Victoria G.; Menezes, Miriam R.; Kim, Hyun-Min; Lobachev, Kirill S.; Stepchenkova, Elena I.; Tahirov, Tahir H.; Rogozin, Igor B.; Pavlov, Youri. I.

    2014-01-01

    The rate of mutations in eukaryotes depends on a plethora of factors and is not immediately derived from the fidelity of DNA polymerases (Pols). Replication of chromosomes containing the anti-parallel strands of duplex DNA occurs through the copying of leading and lagging strand templates by a trio of Pols α, δ and ε, with the assistance of Pol ζ and Y-family Pols at difficult DNA template structures or sites of DNA damage. The parameters of the synthesis at a given location are dictated by the quality and quantity of nucleotides in the pools, replication fork architecture, transcription status, regulation of Pol switches, and structure of chromatin. The result of these transactions is a subject of survey and editing by DNA repair. PMID:23055184

  9. Waste management plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Plant Landfill V, and restore the affected floodplain. The waste management plan addresses management and disposition of all wastes generated during the LEFPC remedial action. Most of the solid wastes will be sanitary or construction/demolition wastes and will be disposed of at existing Y- 12 facilities. Some small amounts of hazardous waste are anticipated, along with possible low-level or mixed wastes (> 35 pCi/g). Liquid wastes will be generated which will be sanitary and capable of being disposed of at the Oak Ridge Sewage Treatment Plant, except sanitary sewage.

  10. Waste management plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Plant Landfill V, and restore the affected floodplain. The waste management plan addresses management and disposition of all wastes generated during the LEFPC remedial action. Most of the solid wastes will be sanitary or construction/demolition wastes and will be disposed of at existing Y- 12 facilities. Some small amounts of hazardous waste are anticipated, along with possible low-level or mixed wastes (> 35 pCi/g). Liquid wastes will be generated which will be sanitary and capable of being disposed of at the Oak Ridge Sewage Treatment Plant, except sanitary sewage

  11. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2015 Progress Report

    International Nuclear Information System (INIS)

    Peterson, Mark J.; Smith, John; Eller, Virginia; DeRolph, Christopher R.

    2016-01-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM) because of large historical losses of mercury within buildings and to soils and surface waters at the Y-12 National Security Complex (Y-12). Because of the extent of mercury losses and the complexities of mercury transport and fate in the downstream environment, the success of conventional options for mercury remediation in lower East Fork Poplar Creek (EFPC) is uncertain. A phased, adaptive management approach to remediation of surface water includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014b).

  12. South Fork Salmon River Watershed Restoration, 2008-2009 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Reaney, Mark D. [Nez Perce Tribe Department of Fisheries Resource Management

    2009-04-15

    The watershed restoration work elements within the project area, the South Fork Salmon River Watershed, follow the watershed restoration approach adopted by the Nez Perce Tribe Department of Fisheries Resource Management (DFRM) - Watershed Division. The vision of the Nez Perce Tribe DFRM-Watershed Division focuses on protecting, restoring, and enhancing watersheds and treaty resources within the ceded territory of the Nez Perce Tribe under the Treaty of 1855 with the United States Federal Government. The program uses a holistic approach, which encompasses entire watersheds, ridge top to ridge top, emphasizing all cultural aspects and strategies that rely on natural fish production and healthy river ecosystems. The Nez Perce Tribe DFRM-Watershed Division strives towards maximizing historic ecosystem productivity and health for the restoration of anadromous and resident fish populations and the habitat on which all depend on for future generations Originally, this project was funded to create a step/pool stream channel that was appropriate to restore fish passage where the 'Glory Hole Cascade' is currently located at the Stibnite Mine. Due to unforeseen circumstances at the time, the project is unable to move forward as planned and a request for a change in scope of the project and an expansion of the geographic area in which to complete project work was submitted. No additional funds were being requested. The ultimate goal of this project is to work with the holistic, ridge top to ridge top approach to protect and restore the ecological and biological functions of the South Fork Salmon River Watershed to assist in the recovery of threatened and endangered anadromous and resident fish species. FY 2008 Work Elements included two aquatic organism passage (AOP) projects to restore habitat connectivity to two fish-bearing tributaries to the East Fork South Fork Salmon River, Salt and Profile Creeks. The Work Elements also included road survey and assessment

  13. Mercury Remediation Technology Development for Lower East Fork Poplar Creek - FY 2015 Progress Report

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, Mark J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Brooks, Scott C. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mathews, Teresa J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mayes, Melanie [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Johs, Alexander [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Watson, David B. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Poteat, Monica D. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Smith, John [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Mehlhorn, Tonia [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Lester, Brian [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Morris, Jesse [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Lowe, Kenneth [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division; Dickson, Johnbull O. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Eller, Virginia [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); DeRolph, Christopher R. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Environmental Sciences Division

    2016-04-01

    Mercury remediation is a high priority for the US Department of Energy (DOE) Oak Ridge Office of Environmental Management (OREM) because of large historical losses of mercury within buildings and to soils and surface waters at the Y-12 National Security Complex (Y-12). Because of the extent of mercury losses and the complexities of mercury transport and fate in the downstream environment, the success of conventional options for mercury remediation in lower East Fork Poplar Creek (EFPC) is uncertain. A phased, adaptive management approach to remediation of surface water includes mercury treatment actions at Y-12 in the short-term and research and technology development (TD) to evaluate longer-term solutions in the downstream environment (US Department of Energy 2014b).

  14. Interface circuit for a multiple-beam tuning-fork gyroscope with high quality factors

    Science.gov (United States)

    Wang, Ren

    This research work presents the design, theoretical analysis, fabrication, interface electronics, and experimental results of a Silicon-On-Insulator (SOI) based Multiple-Beam Tuning-Fork Gyroscope (MB-TFG). Based on a numerical model of Thermo-Elastic Damping (TED), a Multiple-Beam Tuning-Fork Structure (MB-TFS) is designed with high Quality factors (Qs) in its two operation modes. A comprehensive theoretical analysis of the MB-TFG design is conducted to relate the design parameters to its operation parameters and further performance parameters. In conjunction with a mask that defines the device through trenches to alleviate severe fabrication effect on anchor loss, a simple one-mask fabrication process is employed to implement this MB-TFG design on SOI wafers. The fabricated MB-TFGs are tested with PCB-level interface electronics and a thorough comparison between the experimental results and a theoretical analysis is conducted to verify the MB-TFG design and accurately interpret the measured performance. The highest measured Qs of the fabricated MB-TFGs in vacuum are 255,000 in the drive-mode and 103,000 in the sense-mode, at a frequency of 15.7kHz. Under a frequency difference of 4Hz between the two modes (operation frequency is 16.8kHz) and a drive-mode vibration amplitude of 3.0um, the measured rate sensitivity is 80mVpp/°/s with an equivalent impedance of 6MQ. The calculated overall rate resolution of this device is 0.37/hrhiElz, while the measured Angle Random Walk (ARW) and bias instability are 6.67°/'vhr and 95°/hr, respectively.

  15. Tennessee's East Fork Poplar Creek: A biological monitoring and abatement program

    International Nuclear Information System (INIS)

    Halbrook, R.S.; Loar, J.M.; Adams, S.M.; Black, M.C.; Boston, H.L.; Greeley, M.S. Jr.; Hill, W.R.; Hinzman, R.L.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Schilling, E.M.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.; Gatz, A.J.

    1991-01-01

    On May 1985, a Biological Monitoring Program was developed for East Fork Poplar Creek (EFPC) in eastern Tennessee, United States. This stream originates within the Oak Ridge Y-12 Plant that produces nuclear weapons components for the Department of Energy. Water and sediment in the stream contain metals, organic chemicals, and radionuclides from releases that have occurred over the past 45 years. The creek also receives urban and some agricultural runoff and effluent from the City of Oak Ridge's Wastewater Treatment Facility (WTF). The biological monitoring program includes four major tasks: (1) ambient toxicity testing: (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological monitoring of stream communities, including periphyton, benthic macroinvertebrates, and fish. Biological conditions are monitored at six sites on EFPC ranging from kilometer 24.4 near the headwaters to kilometer 6.3 near the month. A site on Brushy Fork, A stream just north of Oak Ridge, is used as reference. Ambient (instream) toxicity was monitored through the use of 7-day static-renewal tests that measured the survival and growth of fathead minnow (Pimephales promelas) larvae and the survival and reproduction of a microstrustacean (Ceriodaphnia dubia). Full-strength water from EFPC within the Y-12 Plant boundary was frequently toxic to Ceriodaphnia, but less frequently toxic to the minnow larvae. Chlorine has been identified as an important toxicant in upper EFPC. Water samples from six sites in EFPC downstream from the Y-12 Plant boundary were tested eight times with both species during a 2-year period (October, 1986 through October, 1988). These sites were ranked by the number of times they were ''best'' or ''worst'' for each species. Water samples collected for use in the ambient toxicity tests were routinely analyzed for conductivity, pH, alkalinity, hardness, total residual and free chlorine, and temperature

  16. Hydrodynamics and Connectivity of Channelized Floodplains: Insights from the Meandering East Fork White River, Indiana, USA

    Science.gov (United States)

    Czuba, J. A.; David, S. R.; Edmonds, D. A.

    2017-12-01

    High resolution topography reveals that meandering river floodplains in Indiana commonly have networks of channels. These floodplain channel networks are most prevalent in agricultural, low-gradient, wide floodplains. It appears that these networks are formed when floodplain channels connect oxbows to each other and the main river channel. Collectively, the channels in the floodplain create an interconnected network of pathways that convey water beginning at flows less than bankfull, and as stage increases, more of the floodplain becomes dissected by floodplain channels. In this work, we quantify the hydrodynamics and connectivity of the flow on the floodplain and in the main channel of the East Fork White River near Seymour, Indiana, USA. We constructed a two-dimensional numerical model using HECRAS of the river-floodplain system from LiDAR data and from main-channel river bathymetry to elucidate the behaviour of these floodplain channels across a range of flows. Model calibration and verification data included stage from a USGS gage, high-water marks at a high and medium flow, and an aerial photograph of inundation in the floodplain channels. The numerical model simulated flow depth and velocity, which was used to quantify connectivity of the floodplain channels, exchange between the main channel and floodplain channels, and residence time of water on the floodplain. Model simulations suggest that the floodplain channels convey roughly 50% of the total flow at what is typically considered "bankfull" flow. Overall, we present a process-based approach for analyzing complex floodplain-river systems where an individual floodplain-river system can be distilled down to a set of characteristic curves. Notably, we map the East Fork White River system to exchange-residence time space and argue that this characterization forms the basis for thinking about morphologic evolution (e.g., sediment deposition and erosion) and biogeochemistry (e.g., nitrate removal) in floodplain

  17. Age determination and feeding habits of Nemipterus japonicus (Bloch, 1791) in the northern Oman Sea

    OpenAIRE

    Afshari, M.; Valinassab, T.; Seifabadi, J.; Kamaly, E.

    2013-01-01

    Age determination and feeding habits of the Japanese threadfin bream, Nemipterus japonicus, was carried out in the northern Oman Sea (Chabahar area), based on 212 specimens collected between September 2009 and May 2010. The minimum and maximum fork length and body weight were measured as 145, 258 mm and 55.31, 288.12 g. The relationship between Body Weight (BW) and Fork Length (FL) for all individuals was estimated as BW= 0.0001×FL2.83 (r2 = 0.9425, n= 212). The Vacuity Index (VI) was 55.2% t...

  18. Mass loading of selected major and trace elements in Lake Fork Creek near Leadville, Colorado, September-October 2001

    Science.gov (United States)

    Walton-Day, Katherine; Flynn, Jennifer L.; Kimball, Briant A.; Runkel, Robert L.

    2005-01-01

    A mass-loading study of Lake Fork Creek of the Arkansas River between Sugarloaf Dam and the mouth was completed in September-October 2001 to help ascertain the following: (1) variation of pH and aqueous constituent concentrations (calcium, sulfate, alkalinity, aluminum, cadmium, copper, iron, manganese, lead, and zinc) and their relation to toxicity standards along the study reach; (2) location and magnitude of sources of metal loading to Lake Fork Creek; (3) amount and locations of metal attenuation; (4) the effect of streamside wetlands on metal transport from contributing mine tunnels; and (5) the effect of organic-rich inflow from the Leadville National Fish Hatchery on water quality in Lake Fork Creek. The study was done in cooperation with the Bureau of Land Management, U.S. Department of Agriculture Forest Service, and U.S. Fish and Wildlife Service. Constituent concentrations and pH showed variable patterns over the study reach. Hardness-based acute and chronic toxicity standards were exceeded for some inflows and some constituents. However, stream concentrations did not exceed standards except for zinc starting in the upper parts of the study reach and extending to just downstream from the inflow from the Leadville National Fish Hatchery. Dilution from that inflow lowered stream zinc concentrations to less than acute and chronic toxicity standards. The uppermost 800 meters of the study reach that contained inflow from the Bartlett, Dinero, and Nelson mine tunnels and the Dinero wetland was the greatest source of loading for manganese and zinc. A middle section of the study reach that extended approximately 2 kilometers upstream from the National Fish Hatchery inflow to just downstream from that inflow was the largest source of aluminum, copper, iron, and lead loading. The loading was partially from the National Fish Hatchery inflow but also from unknown sources upstream from that inflow, possibly ground water. The largest sources for calcium and sulfate

  19. Water-quality trends for selected sampling sites in the upper Clark Fork Basin, Montana, water years 1996-2010

    Science.gov (United States)

    Sando, Steven K.; Vecchia, Aldo V.; Lorenz, David L.; Barnhart, Elliott P.

    2014-01-01

    A large-scale trend analysis was done on specific conductance, selected trace elements (arsenic, cadmium, copper, iron, lead, manganese, and zinc), and suspended-sediment data for 22 sites in the upper Clark Fork Basin for water years 1996–2010. Trend analysis was conducted by using two parametric methods: a time-series model (TSM) and multiple linear regression on time, streamflow, and season (MLR). Trend results for 1996–2010 indicate moderate to large decreases in flow-adjusted concentrations (FACs) and loads of copper (and other metallic elements) and suspended sediment in Silver Bow Creek upstream from Warm Springs. Deposition of metallic elements and suspended sediment within Warm Springs Ponds substantially reduces the downstream transport of those constituents. However, mobilization of copper and suspended sediment from floodplain tailings and stream banks in the Clark Fork reach from Galen to Deer Lodge is a large source of metallic elements and suspended sediment, which also affects downstream transport of those constituents. Copper and suspended-sediment loads mobilized from within this reach accounted for about 40 and 20 percent, respectively, of the loads for Clark Fork at Turah Bridge (site 20); whereas, streamflow contributed from within this reach only accounted for about 8 percent of the streamflow at Turah Bridge. Minor changes in FACs and loads of copper and suspended sediment are indicated for this reach during 1996–2010. Clark Fork reaches downstream from Deer Lodge are relatively smaller sources of metallic elements than the reach from Galen to Deer Lodge. In general, small decreases in loads and FACs of copper and suspended sediment are indicated for Clark Fork sites downstream from Deer Lodge during 1996–2010. Thus, although large decreases in FACs and loads of copper and suspended sediment are indicated for Silver Bow Creek upstream from Warm Springs, those large decreases are not translated to the more downstream reaches largely

  20. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils: LEFPC appendices, volume 1, appendix I-IV

    International Nuclear Information System (INIS)

    1994-09-01

    This document contains Appendix I-IV for the pilot-scale thermal treatment of lower East Fork Poplar Creek floodplain soils. Included are calibration records; quality assurance; soils characterization; pilot scale trial runs

  1. A COMPREHENSIVE NONPOINT SOURCE FIELD STUDY FOR SEDIMENT, NUTRIENTS, AND PATHOGENS IN THE SOUTH FORK BROAD RIVER WATERSHED IN NORTHEAST GEORGIA

    Science.gov (United States)

    This technical report provides a description of the field project design, quality control, the sampling protocols and analysis methodology used, and standard operating procedures for the South Fork Broad River Watershed (SFBR) Total Maximum Daily Load (TMDL) project. This watersh...

  2. An Inventory and Evaluation of Architectural and Engineering Resources of the Big South Fork National River and Recreation Area, Tennessee and Kentucky.

    Science.gov (United States)

    1982-02-25

    coordinated multidisciplinary study of both the architectural and engineering resources of the National Area. Both research b1 orientation and...South Fork just north of Rugby , and traveled through the site where Jamestown, Tennessee, now stands. A third trail, the Chickamauga Path, left the...Thomas Hughes (1881), the founder of the English colony of Rugby , Tennessee, described his neighbors in the Big South Fork area as mostly poor men

  3. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells

    DEFF Research Database (Denmark)

    Ahuja, Akshay K.; Jodkowska, Karolina; Teloni, Federico

    2016-01-01

    Embryonic stem cells (ESCs) represent a transient biological state, where pluripotency is coupled with fast proliferation. ESCs display a constitutively active DNA damage response (DDR), but its molecular determinants have remained elusive. Here we show in cultured ESCs and mouse embryos that H2AX...... these marks of replication stress do not impair the mitotic process and are rapidly lost at differentiation onset. Delaying the G1/S transition in ESCs allows formation of 53BP1 nuclear bodies and suppresses ssDNA accumulation, fork slowing and reversal in the following S-phase. Genetic inactivation of fork...... slowing and reversal leads to chromosomal breakage in unperturbed ESCs. We propose that rapid cell cycle progression makes ESCs dependent on effective replication-coupled mechanisms to protect genome integrity....

  4. Hydrogeology and simulated groundwater flow and availability in the North Fork Red River aquifer, southwest Oklahoma, 1980–2013

    Science.gov (United States)

    Smith, S. Jerrod; Ellis, John H.; Wagner, Derrick L.; Peterson, Steven M.

    2017-09-28

    On September 8, 1981, the Oklahoma Water Resources Board established regulatory limits on the maximum annual yield of groundwater (343,042 acre-feet per year) and equal-proportionate-share (EPS) pumping rate (1.0 acre-foot per acre per year) for the North Fork Red River aquifer. The maximum annual yield and EPS were based on a hydrologic investigation that used a numerical groundwater-flow model to evaluate the effects of potential groundwater withdrawals on groundwater availability in the North Fork Red River aquifer. The Oklahoma Water Resources Board is statutorily required (every 20 years) to update the hydrologic investigation on which the maximum annual yield and EPS were based. Because 20 years have elapsed since the final order was issued, the U.S. Geological Survey, in cooperation with the Oklahoma Water Resources Board, conducted an updated hydrologic investigation and evaluated the effects of potential groundwater withdrawals on groundwater flow and availability in the North Fork Red River aquifer in Oklahoma. This report describes a hydrologic investigation of the North Fork Red River aquifer that includes an updated summary of the aquifer hydrogeology. As part of this investigation, groundwater flow and availability were simulated by using a numerical groundwater-flow model.The North Fork Red River aquifer in Beckham, Greer, Jackson, Kiowa, and Roger Mills Counties in Oklahoma is composed of about 777 square miles (497,582 acres) of alluvium and terrace deposits along the North Fork Red River and tributaries, including Sweetwater Creek, Elk Creek, Otter Creek, and Elm Fork Red River. The North Fork Red River is the primary source of surface-water inflow to Lake Altus, which overlies the North Fork Red River aquifer. Lake Altus is a U.S. Bureau of Reclamation reservoir with the primary purpose of supplying irrigation water to the Lugert-Altus Irrigation District.A hydrogeologic framework was developed for the North Fork Red River aquifer and included a

  5. Bed-material entrainment potential, Roaring Fork River at Basalt, Colorado

    Science.gov (United States)

    Elliott, John G.

    2002-01-01

    The Roaring Fork River at Basalt, Colorado, has a frequently mobile streambed composed of gravel, cobbles, and boulders. Recent urban and highway development on the flood plain, earlier attempts to realign and confine the channel, and flow obstructions such as bridge openings and piers have altered the hydrology, hydraulics, sediment transport, and sediment deposition areas of the Roaring Fork. Entrainment and deposition of coarse sediment on the streambed and in large alluvial bars have reduced the flood-conveying capacity of the river. Previous engineering studies have identified flood-prone areas and hazards related to inundation and high streamflow velocity, but those studies have not evaluated the potential response of the channel to discharges that entrain the coarse streambed. This study builds upon the results of earlier flood studies and identifies some potential areas of concern associated with bed-material entrainment. Cross-section surveys and simulated water-surface elevations from a previously run HEC?RAS model were used to calculate the boundary shear stress on the mean streambed, in the thalweg, and on the tops of adjacent alluvial bars for four reference streamflows. Sediment-size characteristics were determined for surficial material on the streambed, on large alluvial bars, and on a streambank. The median particle size (d50) for the streambed samples was 165 millimeters and for the alluvial bars and bank samples was 107 millimeters. Shear stresses generated by the 10-, 50-, and 100-year floods, and by a more common flow that just inundated most of the alluvial bars in the study reach were calculated at 14 of the cross sections used in the Roaring Fork River HEC?RAS model. The Shields equation was used with a Shields parameter of 0.030 to estimate the critical shear stress for entrainment of the median sediment particle size on the mean streambed, in the thalweg, and on adjacent alluvial bar surfaces at the 14 cross sections. Sediment

  6. Confirmatory Sampling and Analysis Plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-12-01

    This document describes the organization, strategy, and procedures to be used to confirm that mercury concentrations in soils in the remediated areas are statistically less than, or equal to, the cleanup standard of 400 ppm. It focuses on confirming the cleanup of the stretch of the Lower East Fork Popular Creed flowing from Lake Reality at the Y-12 Plant, through the City of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation and its associated flood plain

  7. Strand-Specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast.

    Science.gov (United States)

    Yu, Chuanhe; Gan, Haiyun; Zhang, Zhiguo

    2018-01-01

    DNA replication initiates at DNA replication origins after unwinding of double-strand DNA(dsDNA) by replicative helicase to generate single-stranded DNA (ssDNA) templates for the continuous synthesis of leading-strand and the discontinuous synthesis of lagging-strand. Therefore, methods capable of detecting strand-specific information will likely yield insight into the association of proteins at leading and lagging strand of DNA replication forks and the regulation of leading and lagging strand synthesis during DNA replication. The enrichment and Sequencing of Protein-Associated Nascent DNA (eSPAN), which measure the relative amounts of proteins at nascent leading and lagging strands of DNA replication forks, is a step-wise procedure involving the chromatin immunoprecipitation (ChIP) of a protein of interest followed by the enrichment of protein-associated nascent DNA through BrdU immunoprecipitation. The isolated ssDNA is then subjected to strand-specific sequencing. This method can detect whether a protein is enriched at leading or lagging strand of DNA replication forks. In addition to eSPAN, two other strand-specific methods, (ChIP-ssSeq), which detects potential protein-ssDNA binding and BrdU-IP-ssSeq, which can measure synthesis of both leading and lagging strand, were developed along the way. These methods can provide strand-specific and complementary information about the association of the target protein with DNA replication forks as well as synthesis of leading and lagging strands genome wide. Below, we describe the detailed eSPAN, ChIP-ssSeq, and BrdU-IP-ssSeq protocols.

  8. Confirmatory Sampling and Analysis Plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-12-01

    This document describes the organization, strategy, and procedures to be used to confirm that mercury concentrations in soils in the remediated areas are statistically less than, or equal to, the cleanup standard of 400 ppm. It focuses on confirming the cleanup of the stretch of the Lower East Fork Popular Creed flowing from Lake Reality at the Y-12 Plant, through the City of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation and its associated flood plain.

  9. Assessing the potential for rainbow trout reproduction in tributaries of the Mountain Fork River below Broken Bow Dam, southeastern Oklahoma

    Science.gov (United States)

    Long, James M.; Starks, Trevor A.; Farling, Tyler; Bastarache, Robert

    2016-01-01

    Stocked trout (Salmonidae) in reservoir tailwater systems in the Southern United States have been shown to use tributary streams for spawning and rearing. The lower Mountain Fork of the Little River below Broken Bow Dam is one of two year-round tailwater trout fisheries in Oklahoma, and the only one with evidence of reproduction by stocked rainbow trout (Oncorhynchus mykiss). Whether stocked trout use tributaries in this system for spawning is unknown. Furthermore, an

  10. RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together.

    Science.gov (United States)

    Seeber, Andrew; Hegnauer, Anna Maria; Hustedt, Nicole; Deshpande, Ishan; Poli, Jérôme; Eglinger, Jan; Pasero, Philippe; Gut, Heinz; Shinohara, Miki; Hopfner, Karl-Peter; Shimada, Kenji; Gasser, Susan M

    2016-12-01

    The Mre11-Rad50-Xrs2 (MRX) complex is related to SMC complexes that form rings capable of holding two distinct DNA strands together. MRX functions at stalled replication forks and double-strand breaks (DSBs). A mutation in the N-terminal OB fold of the 70 kDa subunit of yeast replication protein A, rfa1-t11, abrogates MRX recruitment to both types of DNA damage. The rfa1 mutation is functionally epistatic with loss of any of the MRX subunits for survival of replication fork stress or DSB recovery, although it does not compromise end-resection. High-resolution imaging shows that either the rfa1-t11 or the rad50Δ mutation lets stalled replication forks collapse and allows the separation not only of opposing ends but of sister chromatids at breaks. Given that cohesin loss does not provoke visible sister separation as long as the RPA-MRX contacts are intact, we conclude that MRX also serves as a structural linchpin holding sister chromatids together at breaks. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Fork-join and data-driven execution models on multi-core architectures: Case study of the FMM

    KAUST Repository

    Amer, Abdelhalim

    2013-01-01

    Extracting maximum performance of multi-core architectures is a difficult task primarily due to bandwidth limitations of the memory subsystem and its complex hierarchy. In this work, we study the implications of fork-join and data-driven execution models on this type of architecture at the level of task parallelism. For this purpose, we use a highly optimized fork-join based implementation of the FMM and extend it to a data-driven implementation using a distributed task scheduling approach. This study exposes some limitations of the conventional fork-join implementation in terms of synchronization overheads. We find that these are not negligible and their elimination by the data-driven method, with a careful data locality strategy, was beneficial. Experimental evaluation of both methods on state-of-the-art multi-socket multi-core architectures showed up to 22% speed-ups of the data-driven approach compared to the original method. We demonstrate that a data-driven execution of FMM not only improves performance by avoiding global synchronization overheads but also reduces the memory-bandwidth pressure caused by memory-intensive computations. © 2013 Springer-Verlag.

  12. DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression.

    Science.gov (United States)

    Hampp, Stephanie; Kiessling, Tina; Buechle, Kerstin; Mansilla, Sabrina F; Thomale, Jürgen; Rall, Melanie; Ahn, Jinwoo; Pospiech, Helmut; Gottifredi, Vanesa; Wiesmüller, Lisa

    2016-07-26

    DNA damage tolerance facilitates the progression of replication forks that have encountered obstacles on the template strands. It involves either translesion DNA synthesis initiated by proliferating cell nuclear antigen monoubiquitination or less well-characterized fork reversal and template switch mechanisms. Herein, we characterize a novel tolerance pathway requiring the tumor suppressor p53, the translesion polymerase ι (POLι), the ubiquitin ligase Rad5-related helicase-like transcription factor (HLTF), and the SWI/SNF catalytic subunit (SNF2) translocase zinc finger ran-binding domain containing 3 (ZRANB3). This novel p53 activity is lost in the exonuclease-deficient but transcriptionally active p53(H115N) mutant. Wild-type p53, but not p53(H115N), associates with POLι in vivo. Strikingly, the concerted action of p53 and POLι decelerates nascent DNA elongation and promotes HLTF/ZRANB3-dependent recombination during unperturbed DNA replication. Particularly after cross-linker-induced replication stress, p53 and POLι also act together to promote meiotic recombination enzyme 11 (MRE11)-dependent accumulation of (phospho-)replication protein A (RPA)-coated ssDNA. These results implicate a direct role of p53 in the processing of replication forks encountering obstacles on the template strand. Our findings define an unprecedented function of p53 and POLι in the DNA damage response to endogenous or exogenous replication stress.

  13. Quarterly Progress Report - Biological Monitoring Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S.M.; Christensen, S.W.; Greeley, M.S.jr; Hill, W.R.; McCarthy, J.F.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth, G.R.; Stewart, A.J.

    2000-10-18

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 Plant. As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Plant protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Loar et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Plant on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Plant discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities. Monitoring is currently being conducted at five primary EFPC sites, although sites may be excluded or added depending upon the specific objectives of the various tasks. Criteria used in selecting the sites include: (1) location of sampling sites used in other studies, (2) known or suspected sources of downstream impacts, (3) proximity to U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) boundaries, (4) concentration of mercury in the adjacent floodplain, (5) appropriate habitat distribution, and

  14. South Fork Flathead Watershed Westslope Cutthroat Trout Conservation Program, Annual Report 2002.

    Energy Technology Data Exchange (ETDEWEB)

    Grisak, Grant; Marotz, Brian

    2003-06-01

    In 1999, Montana Fish, Wildlife & Parks (MFWP) began a program aimed at conserving the genetically pure populations of westslope cutthroat trout in the South Fork Flathead River drainage. The objective of this program is to eliminate all of the exotic and hybrid trout that threaten the genetically pure westslope cutthroat populations in the South Fork Flathead. The exotic and hybrid trout populations occur in several headwater lakes and their outflow streams. In 2001 MFWP released a draft environmental assessment, pursuant to the Montana Environmental Policy Act (MEPA), that addressed the use of motorized equipment to deliver personnel and materials to some of these lakes in the Bob Marshall and Great Bear Wildernesses (Grisak 2001). After a 30-day public comment period, MFWP determined that the complexity of issues was too great and warranted a more detailed analysis. These issues included transportation options for personnel, equipment and materials, the use of motorized equipment in wilderness, fish removal methods, fish stocking, and the status and distribution of amphibian populations in the project area. Because the program also involves the U.S. Forest Service (USFS) and Bonneville Power Administration (BPA), the environmental analysis needs to comply with the National Environmental Policy Act (NEPA). In October 2001, pursuant to NEPA, MFWP, along with the USFS and BPA initiated an environmental assessment to address these issues. In June 2002, the three agencies determined that the scope of these issues warranted an Environmental Impact Statement. This specialist report describes the logistical, technical and biological issues associated with this project and provides an analysis of options for fish removal, transportation and fish stocking. It further analyzes issues and concerns associated with amphibian populations and creating new domesticated stocks of westslope cutthroat trout. Finally, this document provides a description of each lake, the best

  15. Remedial design work plan for Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-10-01

    The Remedial Design Work Plan (RDWP) for Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee. This remedial action fits into the overall Oak Ridge Reservation (ORR) cleanup strategy by addressing contaminated floodplain soil. The objective of this remedial action is to minimize the risk to human health and the environment from contaminated soil in the Lower EFPC floodplain pursuant to the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) and the Federal Facility Agreement (FFA) (1992). In accordance with the FFA, a remedial investigation (RI) (DOE 1994a) and a feasibility study (DOE 1994b) were conducted to assess contamination of the Lower EFPC and propose remediation alternatives. The remedial investigation determined that the principal contaminant is mercury, which originated from releases during Y-12 Plant operations, primarily between 1953 and 1963. The recommended alternative by the feasibility study was to excavate and dispose of floodplain soils contaminated with mercury above the remedial goal option. Following the remedial investigation/feasibility study, and also in accordance with the FFA, a proposed plan was prepared to more fully describe the proposed remedy.

  16. Post construction report for Lower East Fork Poplar Creek Project, Phase 1, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-11-01

    This Phase 1 Remedial Action (RA) effort was conducted in accordance with the Record of Decision (ROD) for Lower East Fork Poplar Creek (LEFPC) as a Comprehensive Environmental Response, Compensation, and Liability Act action. The LEFPC, Phase 1 RA removed approximately 5,560 yd 3 of mercury-contaminated soils, ≥ 400 ppm, from selected portions of the National Oceanographic and Atmospheric Administration (NOAA) site LEFPC floodplain from July 8, 1996--September 14, 1996. During excavation activities, pockets of elevated radiologically contaminated soils (greater than 35 pCi/g) were located by the continuous monitoring of the excavation areas and contaminated soils with radiological monitoring instruments. Through characterization sampling it has been determined that ∼ 90 yd 3 are less than 35 pCi/g uranium contaminated and will be transported to the Y-12 Landfill V for disposal and the remaining ∼40 yd 3 do not meet the WAC for radiological constituents included in the Special Waste Permit for Landfill V. The radiologically contaminated soil will be placed in 21st Century containers for storage at the K-25 site

  17. CRITICAL CONTROL POINT IDENTIFICATION THROUGH TROPHOLOGICAL MEAT PRODUCTION CHAINFROM FIELD TO FORK

    Directory of Open Access Journals (Sweden)

    A. V. Borodin

    2017-01-01

    Full Text Available  Competitive production management is impossible without comprehensive hazard monitoring and critical parameters control at every stage of food production from raw material and auxiliary materials delivery to ready product realization, which is difficult without modern IT-support. The HACCP (Hazard Analysis and Critical Control Points approach to product safety differs from ready product testing for compliance with NaTD requirements (Normative and Technical Documentation and emphasizes the importance of the process approach to monitoring at every stage of food production. Critical control points (CCP identification is a stage, where the presence of a risk of manufacturing products that are unsafe for human health is recognized and it is possible to take action to its elimination, prevention or reduction to an acceptable level. The use of soſtware package significantly increases the enterprise HACCP system efficiency. The article describes methodological bases for IT-approach to the CCP identification in the trophological meat production chain from field to fork. The algorithmic support and soſtware for the «Decision tree», which allows detecting existing hazards, identifying risks, determining CCPs and describing them, has been developed.

  18. Fight and air exposure times of caught and released salmonids from the South Fork Snake River

    Science.gov (United States)

    Roth, Curtis J.; Schill, Daniel J.; Quist, Michael C.

    2018-01-01

    Catch-and-release regulations are among the most common types of fishing regulations. In recent years, concerns have arisen regarding the exposure of fish to air during catch-and-release angling. The purpose of our study was to quantify the length of time angled fish were exposed to air by anglers in a typical catch-and-release fishery and relate it to the lengths of time reported to produce negative effects. In total, 312 individual anglers were observed on the South Fork Snake River, Idaho, from May through August 2016. Fight time varied from 1.1 s to 230.0 s, and average fight time was 40.0 s (SD = 36.8). Total air exposure times varied from 0.0 s to 91.8 s and averaged 19.3 s (SD = 15.0). Though not statistically significant, a trend in reduced fight times was observed when anglers were guided and increased air exposure times when a net was used and a picture was taken. Results of the current study suggest that anglers expose fish to air for periods that are much less than those reported to cause mortality.

  19. Risk-based decision making: The East Fork Poplar Creek case study

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.R.J.; Sample, B.E.; Suter, G.W.; Parkhurst, B.R.; Teed, R.S.

    1999-12-01

    A probabilistic risk assessment revealed that methylmercury released from the US Department of Energy (DOE) Y-12 weapons facility at Oak Ridge, Tennessee, USA, poses moderate risks to mink and kingfishers residing near the receiving waters of East Fork Poplar Creek. Polychlorinated biphenyls (PCBs) released from this facility pose severe risks to mink but little risk to kingfishers. The objective of this study was to use a risk-based decision-making approach to select remedial cleanup levels for each of these contaminants. The authors conducted Monte Carlo simulations to estimate total daily intakes of each contaminant by mink (mercury and PCBs) and kingfishers (PCBs only) for a range of exposure-reduction scenarios. The resulting exposure distributions were then integrated with their respective dose-response curves to estimate postremediation risks. The results indicated that total mercury levels in surface water would need to be reduced from current levels (mean = 0.225 {micro}g/L) to 0.03 to 0.05 {micro}g/L to reduce risks to very low levels (<5% probability of {ge}20% mortality) for both mink and kingfishers. If interested parties define acceptable risk as, for example, a 20% probability of {gt} 10% mortality, then mercury levels would need to be reduced to 0.14 {micro}g/L. The PCBs analysis indicated that reducing water-borne exposures would produce only a modest reduction in risk to mink because much of the current exposure is through terrestrial exposure pathways.

  20. An inventory of wetlands in the East Fork Poplar Creek floodplain, Anderson and Roane Counties, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-12-01

    An inventory of wetlands within the floodplain of East Fork Poplar Creek (EFPC) in Anderson and Roane Counties, Tennessee was conducted during October, 1991 through May, 1992 for the US Department of Energy (DOE) by the US Army Corps of Engineers, Nashville District. About 15 miles of EFPC channel and 500 acres of its floodplain are contaminated with mercury and other contaminants released from the Y-12 Plant on the DOE Oak Ridge Reservation. The wetland inventory will serve as baseline information for DOE`s remedial action planning and National Environmental Policy Act compliance efforts related to the contamination. In order to provide broad wetland determinations beyond which future wetland definitions are unlikely to expand, the 1989 Federal Manual for Identifying And Delineating Jurisdictional Wetlands was utilized. Using the manual`s methodology in a contaminated system under the approved health and safety plan presented some unique problems, resulting in intrusive sampling for field indicators of hydric soils being accomplished separately from observation of other criteria. Beginning with wetland areas identified on National Wetland Inventory Maps, the entire floodplain was examined for presence of wetland criteria, and 17 wetlands were identified ranging from 0.01 to 2.81 acres in size. The majority of wetlands identified were sized under 1 acre. Some of the wetlands identified were not delineated on the National Wetland Inventory Maps, and much of the wetland area delineated on the maps did not meet the criteria under the 1989 manual.

  1. Vibration-Induced Errors in MEMS Tuning Fork Gyroscopes with Imbalance.

    Science.gov (United States)

    Fang, Xiang; Dong, Linxi; Zhao, Wen-Sheng; Yan, Haixia; Teh, Kwok Siong; Wang, Gaofeng

    2018-05-29

    This paper discusses the vibration-induced error in non-ideal MEMS tuning fork gyroscopes (TFGs). Ideal TFGs which are thought to be immune to vibrations do not exist, and imbalance between two gyros of TFGs is an inevitable phenomenon. Three types of fabrication imperfections (i.e., stiffness imbalance, mass imbalance, and damping imbalance) are studied, considering different imbalance radios. We focus on the coupling types of two gyros of TFGs in both drive and sense directions, and the vibration sensitivities of four TFG designs with imbalance are simulated and compared. It is found that non-ideal TFGs with two gyros coupled both in drive and sense directions (type CC TFGs) are the most insensitive to vibrations with frequencies close to the TFG operating frequencies. However, sense-axis vibrations with in-phase resonant frequencies of a coupled gyros system result in severe error outputs to TFGs with two gyros coupled in the sense direction, which is mainly attributed to the sense capacitance nonlinearity. With increasing stiffness coupled ratio of the coupled gyros system, the sensitivity to vibrations with operating frequencies is cut down, yet sensitivity to vibrations with in-phase frequencies is amplified.

  2. Lessons learned at Lower East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Burch, K.L.; Page, D.G.

    1996-01-01

    The US Department of Energy (DOE) used several innovative strategies and technologies in conducting the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA) activities for the Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee. These innovations helped to cost-effectively characterize the 270-ha (670-acre), 23.3-km (14.5-mile) floodplain and to obtain a 400-parts per million (ppm) cleanup level for mercury in soil. Lessons learned during the project involve management, investigation, and risk assessment strategies and techniques. Management lessons learned include (a) how to handle the large OU, (b) how to effectively involve the community in decisions, and (c) how to select a remedy that incorporates the needs of many involved agencies. Investigation lessons learned include (a) how to design an effective sampling strategy for the site, (b) how to cost-effectively analyze a large number of samples, and (c) which of several treatment technologies is best-suited to the site. Risk assessment lessons learned include (a) how to determine an appropriate cleanup level for human health and the environment, (b) how to quantify uncertainty in the human health risk assessment, (c) how to reconcile different solubilities of different mercury species, and (d) how to best conduct the ecological risk assessment. Other CERCLA sites can benefit from lessons learned during this project whether still in the investigative stage or further along in the process. Applying these lessons can substantially reduce costs and make more efficient use of Superfund resources

  3. Waste Management Plan for the Lower East Fork Poplar Creek Remedial Action Project Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Landfill V, and restore the affected floodplain upon completion of remediation activities. This effort will be conducted in accordance with the Record of Decision (ROD) for LEFPC as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) action. The Waste Management Plan addresses management and disposition of all wastes generated during the remedial action for the LEFPC Project Most of the solid wastes will be considered to be sanitary or construction/demolition wastes and will be disposed of at existing Y-12 facilities for those types of waste. Some small amounts of hazardous waste are anticipated, and the possibility of low- level or mixed waste exists (greater than 35 pCi/g), although these are not expected. Liquid wastes will be generated which will be sanitary in nature and which will be capable of being disposed 0214 of at the Oak Ridge Sewage Treatment Plant.

  4. Directing the path of light-induced electron transfer at a molecular fork using vibrational excitation

    Science.gov (United States)

    Delor, Milan; Archer, Stuart A.; Keane, Theo; Meijer, Anthony J. H. M.; Sazanovich, Igor V.; Greetham, Gregory M.; Towrie, Michael; Weinstein, Julia A.

    2017-11-01

    Ultrafast electron transfer in condensed-phase molecular systems is often strongly coupled to intramolecular vibrations that can promote, suppress and direct electronic processes. Recent experiments exploring this phenomenon proved that light-induced electron transfer can be strongly modulated by vibrational excitation, suggesting a new avenue for active control over molecular function. Here, we achieve the first example of such explicit vibrational control through judicious design of a Pt(II)-acetylide charge-transfer donor-bridge-acceptor-bridge-donor 'fork' system: asymmetric 13C isotopic labelling of one of the two -C≡C- bridges makes the two parallel and otherwise identical donor→acceptor electron-transfer pathways structurally distinct, enabling independent vibrational perturbation of either. Applying an ultrafast UVpump(excitation)-IRpump(perturbation)-IRprobe(monitoring) pulse sequence, we show that the pathway that is vibrationally perturbed during UV-induced electron transfer is dramatically slowed down compared to its unperturbed counterpart. One can thus choose the dominant electron transfer pathway. The findings deliver a new opportunity for precise perturbative control of electronic energy propagation in molecular devices.

  5. Waste Management Plan for the Lower East Fork Poplar Creek Remedial Action Project Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-08-01

    The Lower East Fork Poplar Creek (LEFPC) Remedial Action project will remove mercury-contaminated soils from the floodplain of LEFPC, dispose of these soils at the Y-12 Landfill V, and restore the affected floodplain upon completion of remediation activities. This effort will be conducted in accordance with the Record of Decision (ROD) for LEFPC as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) action. The Waste Management Plan addresses management and disposition of all wastes generated during the remedial action for the LEFPC Project Most of the solid wastes will be considered to be sanitary or construction/demolition wastes and will be disposed of at existing Y-12 facilities for those types of waste. Some small amounts of hazardous waste are anticipated, and the possibility of low- level or mixed waste exists (greater than 35 pCi/g), although these are not expected. Liquid wastes will be generated which will be sanitary in nature and which will be capable of being disposed 0214 of at the Oak Ridge Sewage Treatment Plant

  6. A Network of Multi-Tasking Proteins at the DNA Replication Fork Preserves Genome Stability.

    Directory of Open Access Journals (Sweden)

    2005-12-01

    Full Text Available To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the DNA2-interacting genes defines a network (consisting of 322 genes and 876 interactions whose topology provides clues as to how replication proteins coordinate regulation and repair to protect genome integrity. The results also shed new light on the functions of the query gene DNA2, which, despite many years of study, remain controversial, especially its proposed role in Okazaki fragment processing and the nature of its in vivo substrates. Because of the multifunctional nature of virtually all proteins at the replication fork, the meaning of any single genetic interaction is inherently ambiguous. The multiplexing nature of the current studies, however, combined with follow-up supporting experiments, reveals most if not all of the unique pathways requiring Dna2p. These include not only Okazaki fragment processing and DNA repair but also chromatin dynamics.

  7. Salmonella and Campylobacter biofilm formation: a comparative assessment from farm to fork.

    Science.gov (United States)

    Lamas, Alexandre; Regal, Patricia; Vázquez, Beatriz; Miranda, José M; Cepeda, Alberto; Franco, Carlos M

    2018-02-09

    It takes several steps to bring food from the farm to the fork (dining table), and contamination with food-borne pathogens can occur at any point in the process. Campylobacter spp. and Salmonella spp. are the main microorganisms responsible for foodborne disease in the EU. These two pathogens are able to persist throughout the food supply chain thanks to their ability to form biofilms. Owing to the high prevalence of Salmonella and especially of Campylobacter in the food supply chain and the huge efforts of food authorities to reduce these levels, it is of great importance to fully understand their mechanisms of persistence. Diverse studies have evaluated the biofilm-forming capacity of foodborne pathogens isolated at different steps of food production. Nonetheless, the principal obstacle of these studies is to reproduce the real conditions that microorganisms encounter in the food supply chain. While there are a wide number of Salmonella biofilm studies, information on Campylobacter biofilms is still limited. A comparison between the two microorganisms could help to develop new research in the field of Campylobacter biofilms. Therefore, this review evaluates relevant work in the field of Salmonella and Campylobacter biofilms and the applicability of the data obtained from these studies to real working conditions. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  8. Quartz-Enhanced Photoacoustic Spectroscopy Sensor with a Small-Gap Quartz Tuning Fork

    Directory of Open Access Journals (Sweden)

    Yu-Fei Ma

    2018-06-01

    Full Text Available A highly sensitive quartz-enhanced photoacoustic spectroscopy (QEPAS sensor based on a custom quartz tuning fork (QTF with a small-gap of 200 μm was demonstrated. With the help of the finite element modeling (FEM simulation software COMSOL, the change tendency of the QEPAS signal under the influence of the laser beam vertical position and the length of the micro-resonator (mR were calculated theoretically. Water vapor (H2O was selected as the target analyte. The experimental results agreed well with those of the simulation, which verified the correctness of the theoretical model. An 11-fold signal enhancement was achieved with the addition of an mR with an optimal length of 5 mm in comparison to the bare QTF. Finally, the H2O-QEPAS sensor, which was based on a small-gap QTF, achieved a minimum detection limit (MDL of 1.3 ppm, indicating an improvement of the sensor performance when compared to the standard QTF that has a gap of 300 μm.

  9. Post construction report for Lower East Fork Poplar Creek Project, Phase 1, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-11-01

    This Phase 1 Remedial Action (RA) effort was conducted in accordance with the Record of Decision (ROD) for Lower East Fork Poplar Creek (LEFPC) as a Comprehensive Environmental Response, Compensation, and Liability Act action. The LEFPC, Phase 1 RA removed approximately 5,560 yd{sup 3} of mercury-contaminated soils, {ge} 400 ppm, from selected portions of the National Oceanographic and Atmospheric Administration (NOAA) site LEFPC floodplain from July 8, 1996--September 14, 1996. During excavation activities, pockets of elevated radiologically contaminated soils (greater than 35 pCi/g) were located by the continuous monitoring of the excavation areas and contaminated soils with radiological monitoring instruments. Through characterization sampling it has been determined that {approximately} 90 yd{sup 3} are less than 35 pCi/g uranium contaminated and will be transported to the Y-12 Landfill V for disposal and the remaining {approximately}40 yd{sup 3} do not meet the WAC for radiological constituents included in the Special Waste Permit for Landfill V. The radiologically contaminated soil will be placed in 21st Century containers for storage at the K-25 site.

  10. Solar project description for Design Construction Association single family dwelling, Big Fork, Montana

    Science.gov (United States)

    1980-04-01

    A solar energy system was installed in a 2100 sq ft house located in Big Fork, Montana. The system is designed to provide solar energy for heating and domestic hot water. Solar energy is collected by flat plate collectors with a gross area of 792 square feet. The collector banks are mounted on the roof of the house and face due south at an angle of 45 deg to the horizontal optimizing solar energy collection. Solar energy is transferred from the collector array to a 1500 gallon storage tank. Water is used as the heat collection, transfer and storage medium. Freeze protection is provided by use of a drain down system. Space heating demands are met by circulating hot water from storage through baseboard units in the distribution system of the house. Auxiliary space heating is provided by an electrical heating element in the boiler. Similarly, an electrical heating element in the DHW tank provides energy for water heating. The dwelling was fully instrumented for performance evaluation since October 1977 and the data is integrated into the National Solar Data Network.

  11. Design of a Compact Tuning Fork-Shaped Notched Ultrawideband Antenna for Wireless Communication Application

    Science.gov (United States)

    Shakib, M. N.; Moghavvemi, M.; Mahadi, W. N. L.

    2014-01-01

    A new compact planar notched ultrawideband (UWB) antenna is designed for wireless communication application. The proposed antenna has a compact size of 0.182λ × 0.228λ × 0.018λ where λ is the wavelength of the lowest operating frequency. The antenna is comprised of rectangular radiating patch, ground plane, and an arc-shaped strip in between radiating patch and feed line. By introducing a new Tuning Fork-shaped notch in the radiating plane, a stopband is obtained. The antenna is tested and measured. The measured result indicated that fabricated antenna has achieved a wide bandwidth of 4.33–13.8 GHz (at −10 dB return loss) with a rejection frequency band of 5.28–6.97 GHz (WiMAX, WLAN, and C-band). The effects of the parameters of the antenna are discussed. The experiment results demonstrate that the proposed antenna can well meet the requirement for the UWB communication in spite of its compactness and small size. PMID:24723835

  12. Kinetic energy dissipation of a tuning fork immersed in superfluid helium at different frequencies of oscillations

    International Nuclear Information System (INIS)

    Gritsenko, I.A.; Klokol, K.A.; Sokolov, S.S.; Sheshin, G.A.

    2016-01-01

    An experimental study is made of the drag coefficient, which is the characteristics of energy dissipation during oscillations of the tuning forks, immersed in liquid helium. The experiments were performed in the temperature range from 0.1 to 3.5 K covering both the range of a hydrodynamic flow, and the ballistic regime of transfer of thermal excitations of superfluid helium below 0.6 K. It is found that there is the frequency dependence of the drag coefficient in the hydrodynamic limit, when the main dissipation mechanism is the viscous friction of the fluid against the walls of the oscillating body at temperatures above 0.7 K. In this case, the drag coefficient is proportional to the square root of the frequency of oscillation, and its temperature dependence in He II is determined by the respective dependence of the normal component density of the normal component and the viscosity of the fluid. At lower temperatures, the dependence of drag coefficient on the frequency is not available, and the magnitude of the dissipative losses is determined only by the temperature dependence of the density of the normal component. At the same time in the entire range of temperatures value of dissipative losses depends on the geometry of the oscillating body.

  13. U.S. Geological Survey 2013 assessment of undiscovered resources in the Bakken and Three Forks Formations of the U.S. Williston Basin Province

    Science.gov (United States)

    Gaswirth, Stephanie B.; Marra, Kristen R.

    2014-01-01

    The Upper Devonian Three Forks and Upper Devonian to Lower Mississippian Bakken Formations comprise a major United States continuous oil resource. Current exploitation of oil is from horizontal drilling and hydraulic fracturing of the Middle Member of the Bakken and upper Three Forks, with ongoing exploration of the lower Three Forks, and the Upper, Lower, and Pronghorn Members of the Bakken Formation. In 2008, the U.S. Geological Survey (USGS) estimated a mean of 3.65 billion bbl of undiscovered, technically recoverable oil resource within the Bakken Formation. The USGS recently reassessed the Bakken Formation, which included an assessment of the underlying Three Forks Formation. The Pronghorn Member of the Bakken Formation, where present, was included as part of the Three Forks assessment due to probable fluid communication between reservoirs. For the Bakken Formation, five continuous and one conventional assessment units (AUs) were defined. These AUs are modified from the 2008 AU boundaries to incorporate expanded geologic and production information. The Three Forks Formation was defined with one continuous and one conventional AU. Within the continuous AUs, optimal regions of hydrocarbon recovery, or “sweet spots,” were delineated and estimated ultimate recoveries were calculated for each continuous AU. Resulting undiscovered, technically recoverable resource estimates were 3.65 billion bbl for the five Bakken continuous oil AUs and 3.73 billion bbl for the Three Forks Continuous Oil AU, generating a total mean resource estimate of 7.38 billion bbl. The two conventional AUs are hypothetical and represent a negligible component of the total estimated resource (8 million barrels of oil).

  14. Do Knickpoints Unzip Watersheds? Longitudinal Observations of Terrace and Hillslope Response to Mainstem Incision along the South Fork Eel River, California

    Science.gov (United States)

    Wehrs, K.; Crosby, B. T.

    2017-12-01

    River response to changes in climate and relative base level often leave behind a legacy of transient landforms that enable the interpretation of past events. The dominant paradigm is that base level fall initiates a wave of mainstem incision that progressively transmits change upstream. Mainstem-adjacent hillslopes coupled to the channel subsequently respond as their toe slopes are steepened. To test this paradigm, we first use a longitudinal set of mainstem terrace ages to evaluate whether incision incrementally progresses upstream or is contemporaneous. Second, we explore longitudinal variations in mainstem-adjacent mass movements to evaluate whether they reflect a time and space progression in response. The South Fork Eel River in northern California contains over 600 mainstem-adjacent mass movements and 60 m tall, longitudinally extensive strath terraces that record a landscape response to river incision. We use Optically Stimulated Luminescence, with feldspars and coarse-grained sampling technique, to determine the depositional age of alluvial fill atop the strath terrace. If terrace abandonment progressively young upstream, this suggests that base level fall was not spatially contemporaneous, but rather time progressive. As a consequence, the age, form, and extent of mass wasting events should also vary longitudinally. Because terraces isolate hillslopes from the base level fall signal, we use these surfaces to quantify hillslope form and function independent of that forcing. Preliminary results using mainstem-parallel, 1 m LiDAR, show significant variation in size of mass movements throughout the basin, with planar, linearly moving translational landslides dominating throughout the catchment. In the lower basin, well downstream of the current knickzone, we see an increase in mass movement concentration, reactivation, and overall extent of mass movements. Multiple factors confound our interpretation of hillslope morphology and response, due to changes in

  15. Age at sexual maturity, sex ratio, fecundity, and longevity of isolated headwater populations of westslope cutthroat trout

    Science.gov (United States)

    Christopher C. Downs; Robert G. White; Bradley B. Shepard

    1997-01-01

    We sampled 19 isolated headwater populations of westslope cutthroat trout Oncorhynchus clarki lewisi in Montana to provide estimates of fecundity, longevity, sex ratio, and age at sexual maturity. Fecundity was estimated for 31 fish collected from two streams in the upper Missouri River drainage. Females smaller than 149 mm fork length (FL) were generally immature and...

  16. Quarterly Progress Report - Biological Monitoring Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Adams, S. M.; Christensen, S. W.; Greeley, M.S. jr; McCracken, M.K.; Peterson, M.J.; Ryon, M.G.; Smith, J.G.; Southworth G. R.; Stewart, A. J.

    2001-01-19

    In May 1985, a National Pollutant Discharge Elimination System (NPDES) permit was issued for the Oak Ridge Y-12 National Security Complex (formerly the Oak Ridge Y-12 Plant). As a condition of the permit, a Biological Monitoring and Abatement Program (BMAP) was developed to demonstrate that the effluent limitations established for the Y-12 Complex protect the classified uses of the receiving stream (East Fork Poplar Creek; EFPC), in particular, the growth and propagation of aquatic life (Loar et al. 1989). A second objective of the BMAP is to document the ecological effects resulting from the implementation of a water pollution control program designed to eliminate direct discharges of wastewaters to EFPC and to minimize the inadvertent release of pollutants to the environment. Because of the complex nature of the discharges to EFPC and the temporal and spatial variability in the composition of the discharges, a comprehensive, integrated approach to biological monitoring was developed. A new permit was issued to the Y-12 Complex on April 28, 1995 and became effective on July 1, 1995. Biological monitoring continues to be required under the new permit. The BMAP consists of four major tasks that reflect different but complementary approaches to evaluating the effects of the Y-12 Complex discharges on the aquatic integrity of EFPC. These tasks are (1) toxicity monitoring, (2) biological indicator studies, (3) bioaccumulation studies, and (4) ecological surveys of the periphyton, benthic macroinvertebrate, and fish communities. Monitoring is currently being conducted at five primary EFPC sites, although sites may be excluded or added depending upon the specific objectives of the various tasks. Criteria used in selecting the sites include: (1) location of sampling sites used in other studies, (2) known or suspected sources of downstream impacts, (3) proximity to U.S. Department of Energy (DOE) Oak Ridge Reservation (ORR) boundaries, (4) concentration of mercury in the

  17. Human and ecological remediation goals for soil mercury at East Fork Poplar Creek, Oak Ridge, TN

    International Nuclear Information System (INIS)

    Zafran, F.A.; Cornaby, B.W.; Hadden, C.T.

    1995-01-01

    Mercury, used in the past production of enriched lithium by the Department of Energy, is the principal chemical of concern in the 14-mile floodplain of East Fork Poplar Creek (EFPC). SAIC has developed risk-based remediation goal options (RGOS) for mercury in EFPC soils to protect the most sensitive human receptors. The existing chronic oral RfD for mercury is based on exposure of laboratory species to mercuric chloride. However, speciation and leaching/availability studies (conducted by EPA EMSL and Oak Ridge National Laboratory) indicated less soluble and less toxic mercury species, principally mercuric sulfide, with measurable quantities of metallic mercury also present, predominate in EFPC floodplain soils. SAIC derived human health RGOs using deterministic and probabilistic methods and incorporated the probability density function for bioavailability of mercury species from leaching/availability data generated by ORNL. Monte Carlo simulation was used in uncertainty analysis and supported the derivation of a protective, but realistic risk-based remediation goal of 400 mg mercury/kg soil. For ecological risk assessment, RGOs were based on risks through food chains from contaminants in soil. The authors describe a terrestrial food-chain model of contaminant transfer to primary producers, first-order consumers, mid-level predators, and top-level predators. The model uses published toxicity data, site-specific contaminant concentrations, and bioaccumulation factors calculated from measured body burdens of floodplain organisms to compute RGOs for various combinations of exposure parameters. Model calculations show that under reasonably conservative conditions, mid-level predators have the highest exposures relative to dietary limits and, therefore, require the lowest soil-mercury RGOs. Mercury concentrations of ∼500 mg/kg are protective of the receptor populations exposed through food chains at this site

  18. The Poultry-Associated Microbiome: Network Analysis and Farm-to-Fork Characterizations

    Science.gov (United States)

    Oakley, Brian B.; Morales, Cesar A.; Line, J.; Berrang, Mark E.; Meinersmann, Richard J.; Tillman, Glenn E.; Wise, Mark G.; Siragusa, Gregory R.; Hiett, Kelli L.; Seal, Bruce S.

    2013-01-01

    Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS), quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of longitudinal data following two flocks from the farm through processing showed a core microbiome containing multiple sequence types most closely related to genera known to be pathogenic for animals and/or humans, including Campylobacter, Clostridium, and Shigella. After the final stage of commercial poultry processing, taxonomic richness was ca. 2–4 times lower than the richness of fecal samples from the same flocks and Campylobacter abundance was significantly reduced. Interestingly, however, carcasses sampled at 48 hr after processing harboured the greatest proportion of unique taxa (those not encountered in other samples), significantly more than expected by chance. Among these were anaerobes such as Prevotella, Veillonella, Leptrotrichia, and multiple Campylobacter sequence types. Retail products were dominated by Pseudomonas, but also contained 27 other genera, most of which were potentially metabolically active and encountered in on-farm samples. Network analysis was focused on the foodborne pathogen Campylobacter and revealed a majority of sequence types with no significant interactions with other taxa, perhaps explaining the limited efficacy of previous attempts at competitive exclusion of Campylobacter. These data represent the first use of HTS to characterize the poultry microbiome across a series of farm-to-fork samples and demonstrate the utility of HTS in monitoring the food supply chain and identifying sources of potential zoonoses and interactions among taxa in complex communities. PMID:23468931

  19. Quartz tuning fork based sensor for detection of volatile organic compounds: towards breath analysis

    Science.gov (United States)

    Sampson, Abraham; Panchal, Suresh; Phadke, Apoorva; Kashyap, A.; Suman, Jilma; Unnikrishnan, G.; Datar, Suwarna

    2018-04-01

    Several volatile organic compounds (VOCs) are present in the exhaled human breath whose concentration can vary depending on the physiological changes occurring within a human being. These changes in the concentration or the occurrence of a particular VOC can be used as signature of a particular disease in a person. In the present work, a sensor has been developed to detect VOCs such as 1,4-dimethoxy-2,3-butanediol (BD), and cyclohexanone (CH), acetone, methanol and ethanol. Except for BD and CH, the rest of the VOCs are present in a healthy person in ppm levels. CH and BD have been reported to be present in the exhaled human breath of breast cancer patients in ppm levels and can be used to distinguish between a healthy person and a person with breast cancer. The selectivity of the sensor towards these two compounds in the presence of other VOCs commonly present in human breath like acetone, ethanol and methanol has been studied. The sensor has been developed using modified Quartz Tuning Forks (QTFs) with the intent of developing an array of such sensors identifying different VOCs present in a healthy human’s breath. Two differently modified QTFs have been used to detect 1 ppm of 1,4-dimethoxy-2,3-butanediol and 20 ppm of cyclohexanone. Linear Discriminants Analysis (LDA) has been used to classify seven different VOCs. For this purpose, features extracted from sensor responses -shift in resonant frequency, response time and recovery time of the sensors- have been used as features in the model. Differently modified array of QTFs along with the use of LDA can be a useful pathway towards development of a QTF based sensor array for human breath analysis.

  20. Floodplain Vegetation Productivity and Carbon Cycle Dynamics of the Middle Fork Flathead River of Northwest Montana

    Science.gov (United States)

    Oakins, A. J.; Kimball, J. S.; Relyea, S.; Stanford, J. A.

    2005-05-01

    River floodplains are vital natural features that store floodwaters, improve water quality, provide habitat, and create recreational opportunities. Recent studies have shown that strong interactions among flooding, channel and sediment movement, vegetation, and groundwater create a dynamic shifting habitat mosaic that promotes biodiversity and complex food webs. Multiple physical and environmental processes interact within these systems to influence forest productivity, including water availability, nutrient supply, soil texture, and disturbance history. This study is designed to quantify the role of groundwater depth and meteorology in determining spatial and temporal patterns of net primary productivity (NPP) within the Nyack floodplain of the Middle Fork Flathead River, Northwestern Montana. We examine three intensive field sites composed of mature, mixed deciduous and evergreen conifer forest with varying hydrologic and vegetative characteristics. We use a modified Biome-BGC ecosystem process model with field-collected data (LAI, increment growth cores, groundwater depth, vegetation sap-flow, and local meteorology) to describe the effects of floodplain groundwater dynamics on vegetation community structure, and carbon/nitrogen cycling. Initial results indicate that conifers are more sensitive than deeper-rooted deciduous species to variability in groundwater depth and meteorological conditions. Forest productivity also shows a non-linear response to groundwater depth. Sites with intermediate groundwater depths (0.2-0.5m) allow vegetation to maintain connectivity to groundwater over longer periods during the growing season, are effectively uncoupled from atmospheric constraints on photosynthesis, and generally have greater productivity. Shallow groundwater sites (<0.2m) are less productive due to the indirect effects of reduced soil aerobic decomposition and reduced plant available nitrogen.

  1. The poultry-associated microbiome: network analysis and farm-to-fork characterizations.

    Directory of Open Access Journals (Sweden)

    Brian B Oakley

    Full Text Available Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS, quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of longitudinal data following two flocks from the farm through processing showed a core microbiome containing multiple sequence types most closely related to genera known to be pathogenic for animals and/or humans, including Campylobacter, Clostridium, and Shigella. After the final stage of commercial poultry processing, taxonomic richness was ca. 2-4 times lower than the richness of fecal samples from the same flocks and Campylobacter abundance was significantly reduced. Interestingly, however, carcasses sampled at 48 hr after processing harboured the greatest proportion of unique taxa (those not encountered in other samples, significantly more than expected by chance. Among these were anaerobes such as Prevotella, Veillonella, Leptrotrichia, and multiple Campylobacter sequence types. Retail products were dominated by Pseudomonas, but also contained 27 other genera, most of which were potentially metabolically active and encountered in on-farm samples. Network analysis was focused on the foodborne pathogen Campylobacter and revealed a majority of sequence types with no significant interactions with other taxa, perhaps explaining the limited efficacy of previous attempts at competitive exclusion of Campylobacter. These data represent the first use of HTS to characterize the poultry microbiome across a series of farm-to-fork samples and demonstrate the utility of HTS in monitoring the food supply chain and identifying sources of potential zoonoses and interactions among taxa in complex communities.

  2. Remedial design work plan for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-02-01

    The Remedial Design Work Plan (RDWP) for the Lower East Fork Poplar Creek (EFPC) Operable Unit (OU) in Oak Ridge, Tennessee, has been prepared. The remedial investigation determined that the principal contaminant is mercury, which originated from releases during Y-12 Plant operations, primarily between 1953 and 1963. The recommended alternative, as stated in the Record of Decision (ROD) was to excavate and dispose of floodplain soils contaminated with mercury above the remedial goal option. Thereafter, a public hearing was held to review the proposed plan. Comments were incorporated. The revised selected remedy, per the ROD is to excavate and dispose of floodplain soils contaminated above the remediation goal of 400 parts per million mercury. The approved ROD with this goal will be the basis for remedial design (RD). The RD work plan (RDWP) is composed of six chapters. An introductory chapter describes the purpose and scope of the RDWP, the selected remedy as identified by the ROD; the roles and responsibilities of the RD team members, and the site background information, including site history, contaminants of concern, and site characteristics. Chapter 2 contains the design objectives, RD approach, regulatory considerations during RD, and the design criteria with assumptions. Chapter 3 presents the RD planning process to prepare this RDWP, as well as secondary RD support plans. Chapter 4 describes the scope of the RD activities in more detail and identifies what will be included in the design package. Chapter 5 presents the schedule for performance of the RD activities, identifying key RD milestones. Specific documents used in the preparation of this document are referenced in Chapter 6

  3. Groundwater and climate change: a sensitivity analysis for the Grand Forks aquifer, southern British Columbia, Canada

    Science.gov (United States)

    Allen, D. M.; Mackie, D. C.; Wei, M.

    The Grand Forks aquifer, located in south-central British Columbia, Canada was used as a case study area for modeling the sensitivity of an aquifer to changes in recharge and river stage consistent with projected climate-change scenarios for the region. Results suggest that variations in recharge to the aquifer under the different climate-change scenarios, modeled under steady-state conditions, have a much smaller impact on the groundwater system than changes in river-stage elevation of the Kettle and Granby Rivers, which flow through the valley. All simulations showed relatively small changes in the overall configuration of the water table and general direction of groundwater flow. High-recharge and low-recharge simulations resulted in approximately a +0.05 m increase and a -0.025 m decrease, respectively, in water-table elevations throughout the aquifer. Simulated changes in river-stage elevation, to reflect higher-than-peak-flow levels (by 20 and 50%), resulted in average changes in the water-table elevation of 2.72 and 3.45 m, respectively. Simulated changes in river-stage elevation, to reflect lower-than-baseflow levels (by 20 and 50%), resulted in average changes in the water-table elevation of -0.48 and -2.10 m, respectively. Current observed water-table elevations in the valley are consistent with an average river-stage elevation (between current baseflow and peak-flow stages). L'aquifère de Grand Forks, situé en Colombie britannique (Canada), a été utilisé comme zone d'étude pour modéliser la sensibilité d'un aquifère à des modifications de la recharge et du niveau de la rivière, correspondant à des scénarios envisagés de changement climatique dans cette région. Les résultats font apparaître que les variations de recharge de l'aquifère pour différents scénarios de changement climatique, modélisées pour des conditions de régime permanent, ont un impact sur le système aquifère beaucoup plus faible que les changements du niveau des

  4. A ruthenium polypyridyl intercalator stalls DNA replication forks, radiosensitizes human cancer cells and is enhanced by Chk1 inhibition

    Science.gov (United States)

    Gill, Martin R.; Harun, Siti Norain; Halder, Swagata; Boghozian, Ramon A.; Ramadan, Kristijan; Ahmad, Haslina; Vallis, Katherine A.

    2016-08-01

    Ruthenium(II) polypyridyl complexes can intercalate DNA with high affinity and prevent cell proliferation; however, the direct impact of ruthenium-based intercalation on cellular DNA replication remains unknown. Here we show the multi-intercalator [Ru(dppz)2(PIP)]2+ (dppz = dipyridophenazine, PIP = 2-(phenyl)imidazo[4,5-f][1,10]phenanthroline) immediately stalls replication fork progression in HeLa human cervical cancer cells. In response to this replication blockade, the DNA damage response (DDR) cell signalling network is activated, with checkpoint kinase 1 (Chk1) activation indicating prolonged replication-associated DNA damage, and cell proliferation is inhibited by G1-S cell-cycle arrest. Co-incubation with a Chk1 inhibitor achieves synergistic apoptosis in cancer cells, with a significant increase in phospho(Ser139) histone H2AX (γ-H2AX) levels and foci indicating increased conversion of stalled replication forks to double-strand breaks (DSBs). Normal human epithelial cells remain unaffected by this concurrent treatment. Furthermore, pre-treatment of HeLa cells with [Ru(dppz)2(PIP)]2+ before external beam ionising radiation results in a supra-additive decrease in cell survival accompanied by increased γ-H2AX expression, indicating the compound functions as a radiosensitizer. Together, these results indicate ruthenium-based intercalation can block replication fork progression and demonstrate how these DNA-binding agents may be combined with DDR inhibitors or ionising radiation to achieve more efficient cancer cell killing.

  5. The effect of real-time vibrotactile feedback delivered through an augmented fork on eating rate, satiation, and food intake.

    Science.gov (United States)

    Hermans, Roel C J; Hermsen, Sander; Robinson, Eric; Higgs, Suzanne; Mars, Monica; Frost, Jeana H

    2017-06-01

    Eating rate is a basic determinant of appetite regulation, as people who eat more slowly feel sated earlier and eat less. Without assistance, eating rate is difficult to modify due to its automatic nature. In the current study, participants used an augmented fork that aimed to decelerate their rate of eating. A total of 114 participants were randomly assigned to the Feedback Condition (FC), in which they received vibrotactile feedback from their fork when eating too fast (i.e., taking more than one bite per 10 s), or a Non-Feedback Condition (NFC). Participants in the FC took fewer bites per minute than did those in the NFC. Participants in the FC also had a higher success ratio, indicating that they had significantly more bites outside the designated time interval of 10 s than did participants in the NFC. A slower eating rate, however, did not lead to a significant reduction in the amount of food consumed or level of satiation. These findings indicate that real-time vibrotactile feedback delivered through an augmented fork is capable of reducing eating rate, but there is no evidence from this study that this reduction in eating rate is translated into an increase in satiation or reduction in food consumption. Overall, this study shows that real-time vibrotactile feedback may be a viable tool in interventions that aim to reduce eating rate. The long-term effectiveness of this form of feedback on satiation and food consumption, however, awaits further investigation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method)

    International Nuclear Information System (INIS)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal

    2016-01-01

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  7. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method).

    Science.gov (United States)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal

    2016-06-01

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  8. Scanning thermal microscopy based on a quartz tuning fork and a micro-thermocouple in active mode (2ω method)

    Energy Technology Data Exchange (ETDEWEB)

    Bontempi, Alexia; Nguyen, Tran Phong; Salut, Roland; Thiery, Laurent; Teyssieux, Damien; Vairac, Pascal [FEMTO-ST Institute UMR 6174, Université de Franche-Comté, CNRS, ENSMM, UTBM, 15B Avenue des Montboucons, F-25030 Besançon (France)

    2016-06-15

    A novel probe for scanning thermal microscope using a micro-thermocouple probe placed on a Quartz Tuning Fork (QTF) is presented. Instead of using an external deflection with a cantilever beam for contact detection, an original combination of piezoelectric resonator and thermal probe is employed. Due to a non-contact photothermal excitation principle, the high quality factor of the QTF allows the probe-to-surface contact detection. Topographic and thermal scanning images obtained on a specific sample points out the interest of our system as an alternative to cantilevered resistive probe systems which are the most spread.

  9. Flood-inundation maps for an 8.9-mile reach of the South Fork Little River at Hopkinsville, Kentucky

    Science.gov (United States)

    Lant, Jeremiah G.

    2013-01-01

    Digital flood-inundation maps for an 8.9-mile reach of South Fork Little River at Hopkinsville, Kentucky, were created by the U.S. Geological Survey (USGS) in cooperation with the City of Hopkinsville Community Development Services. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage at South Fork Little River at Highway 68 By-Pass at Hopkinsville, Kentucky (station no. 03437495). Current conditions for the USGS streamgage may be obtained online at the USGS National Water Information System site (http://waterdata.usgs.gov/nwis/inventory?agency_code=USGS&site_no=03437495). In addition, the information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service flood warning system (http://water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often co-located at USGS streamgages. The forecasted peak-stage information, also available on the Internet, may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the South Fork Little River reach by using HEC-RAS, a one-dimensional step-backwater model developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated by using the most current (2012) stage-discharge relation at the South Fork Little River at Highway 68 By-Pass at Hopkinsville, Kentucky, streamgage and measurements collected during recent flood events. The calibrated model was then used to calculate 13 water-surface profiles for a sequence of flood stages, most at 1-foot intervals, referenced to the streamgage datum and ranging from a stage near bank full to the estimated elevation of the 1.0-percent annual exceedance

  10. Environmental compliance plan for the Lower East Fork Poplar Creek Remedial Action Project at Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-07-01

    Remedial action for Lower East Fork Poplar Creek, as defined by the Record of Decision, requires that soil contaminated with >400 ppM mercury be excavated and disposed. Based on the remediation goal, soil will be excavated from areas located at the NOAA site and the Bruner site and disposed at the Industrial Landfill V at the Y-12 Plant. Objective is to minimize the risk to human health and the environment from contaminated soil in the lower EFPC floodplain pursuant to CERCLA and the Federal Facility Agreement (DOE 1992)

  11. Characterization and 3D reservoir modelling of fluvial sandstones of the Williams Fork Formation, Rulison Field, Piceance Basin, Colorado, USA

    International Nuclear Information System (INIS)

    Pranter, Matthew J; Vargas, Marielis F; Davis, Thomas L

    2008-01-01

    This study describes the stratigraphic characteristics and distribution of fluvial deposits of the Upper Cretaceous Williams Fork Formation in a portion of Rulison Field and addresses 3D geologic modelling of reservoir sand bodies and their associated connectivity. Fluvial deposits include isolated and stacked point-bar deposits, crevasse splays and overbank (floodplain) mudrock. Within the Williams Fork Formation, the distribution and connectivity of fluvial sandstones significantly impact reservoir productivity and ultimate recovery. The reservoir sandstones are primarily fluvial point-bar deposits interbedded with shales and coals. Because of the lenticular geometry and limited lateral extent of the reservoir sandstones (common apparent widths of ∼500–1000 ft; ∼150–300 m), relatively high well densities (e.g. 10 acre (660 ft; 200 m) spacing) are often required to deplete the reservoir. Heterogeneity of these fluvial deposits includes larger scale stratigraphic variability associated with vertical stacking patterns and structural heterogeneities associated with faults that exhibit lateral and reverse offsets. The discontinuous character of the fluvial sandstones and lack of distinct marker beds in the middle and upper parts of the Williams Fork Formation make correlation between wells tenuous, even at a 10 acre well spacing. Some intervals of thicker and amalgamated sandstones within the middle and upper Williams Fork Formation can be correlated across greater distances. To aid correlation and for 3D reservoir modelling, vertical lithology proportion curves were used to estimate stratigraphic trends and define the stratigraphic zonation within the reservoir interval. Object-based and indicator-based modelling methods have been applied to the same data and results from the models were compared. Results from the 3D modelling indicate that sandstone connectivity increases with net-to-gross ratio and, at lower net-to-gross ratios (<30%), differences exist in

  12. Baseline and Postremediation Monitoring Program Plan for the Lower East Fork Poplar Creek operable unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This report was prepared in accordance with CERCLA requirements to present the plan for baseline and postremediation monitoring as part of the selected remedy. It provides the Environmental Restoration Program with information about the requirements to monitor for soil and terrestrial biota in the Lower East Fork Poplar Creek (LEFPC) floodplain; sediment, surface water, and aquatic biota in LEFPC; wetland restoration in the LEFPC floodplain; and human use of shallow groundwater wells in the LEFPC floodplain for drinking water. This document describes the monitoring program that will ensure that actions taken under Phases I and II of the LEFPC remedial action are protective of human health and the environment

  13. Twelve Years of Monitoring Phosphorus and Suspended-Solids Concentrations and Yields in the North Fork Ninnescah River above Cheney Reservoir, South-Central Kansas 1997-2008

    Science.gov (United States)

    Stone, Mandy L.; Graham, Jennifer L.; Ziegler, Andrew C.

    2009-01-01

    Cheney Reservoir, located on the North Fork Ninnescah River in south-central Kansas, is the primary water supply for the city of Wichita and an important recreational resource. Concerns about taste-and-odor occurrences in Cheney Reservoir have drawn attention to potential pollutants, including total phosphorus (TP) and total suspended solids (TSS). July 2009 was the 15th anniversary of the establishment of the Cheney Reservoir Watershed pollution management plan. The U.S. Geological Survey (USGS), in cooperation with the city of Wichita, has collected water-quality data in the basin since 1996, and has monitored water quality continuously on the North Fork Ninnescah River since 1998. This fact sheet describes 12 years (1997-2008) of computed TP and TSS data and compares these data with water-quality goals for the North Fork Ninnescah River, the main tributary to Cheney Reservoir.

  14. Hydraulic, geomorphic, and trout habitat conditions of the Lake Fork of the Gunnison River in Hinsdale County, Lake City, Colorado, Water Years 2010-2011

    Science.gov (United States)

    Williams, Cory A.; Richards, Rodney J.; Schaffrath, Keelin R.

    2015-01-01

    Channel rehabilitation, or reconfiguration, to mitigate a variety of riverine problems has become a common practice in the western United States. However, additional work to monitor and assess the channel response to, and the effectiveness of, these modifications over longer periods of time (decadal or longer) is still needed. The Lake Fork of the Gunnison River has been an area of active channel modification to accommodate the needs of the Lake City community since the 1950s. The Lake Fork Valley Conservancy District began a planning process to assess restoration options for a reach of the Lake Fork in Lake City to enhance hydraulic and ecologic characteristics of the reach. Geomorphic channel form is affected by land-use changes within the basin and geologic controls within the reach. The historic channel was defined as a dynamic, braided channel with an active flood plain. This can result in a natural tendency for the channel to braid. A braided channel can affect channel stability of reconfigured reaches when a single-thread meandering channel is imposed on the stream. The U.S. Geological Survey, in cooperation with the Colorado Water Conservation Board and Colorado River Water Conservation District, began a study in 2010 to quantify existing hydraulic and habitat conditions for a reach of the Lake Fork of the Gunnison River in Lake City, Colorado. The purpose of this report is to quantify existing Lake Fork hydraulic and habitat conditions and establish a baseline against which post-reconfiguration conditions can be compared. This report (1) quantifies the existing hydraulic and geomorphic conditions in a 1.1-kilometer section of the Lake Fork at Lake City that has been proposed as a location for future channel-rehabilitation efforts, (2) characterizes the habitat suitability of the reach for two trout species based on physical conditions within the stream, and (3) characterizes the current riparian canopy density.

  15. Development and Validation of a Simple Analytical Model of the Proton Exchange Membrane Fuel Cell (Pemfc) in a Fork-Lift Truck Power System

    DEFF Research Database (Denmark)

    Hosseinzadeh, Elham; Rokni, Masoud

    2013-01-01

    In this study, a general proton exchange membrane fuel cell (PEMFC) model has been developed in order to investigate the balance of plant of a fork-lift truck thermodynamically. The model takes into account the effects of pressure losses, water crossovers, humidity aspects, and voltage overpotent......In this study, a general proton exchange membrane fuel cell (PEMFC) model has been developed in order to investigate the balance of plant of a fork-lift truck thermodynamically. The model takes into account the effects of pressure losses, water crossovers, humidity aspects, and voltage...

  16. New approach to Fork measurements data analysis by RADAR-CRISP and ORIGEN integration

    International Nuclear Information System (INIS)

    Vaccaro, S.; Svedkauskaite, J.; Smejkal, A.; Schwalbach, P.; De Baere, P.; Hu, J.; Gauld, I.C.

    2013-06-01

    Currently, in the EU, activities related to interim storage of spent fuel are constantly increasing. This is particularly true in Finland and Sweden, where final geological repository sites are planned to be operational in 2023 and 2026 respectively, but also in several other countries where fuel is moved from wet ponds to dry storage (Germany, Belgium, Spain, Czech, Bulgaria, etc). The required verification activities present a considerable challenge to the EURATOM Safeguards authority.. Both EURATOM and IAEA safeguards need to know what is in the storage casks and keep continuity of knowledge of the spent fuel. A frequently-used tool for the verification of the nuclear material during loading is the 'Fork' detectors for gross gamma and neutron counting. The IT applications RADAR (Remote Acquisition of Data and Review) and CRISP (Central RADAR Inspection Support Package), developed by EURATOM, are used to acquire safeguards measurement data and to analyze them in order to verify the declarations of the nuclear plant operators. Under the framework of the U.S. DOE-EURATOM Agreement on nuclear safeguards and security, a module for automated analysis of spent fuel measurement data using the ORIGEN (Oak Ridge Isotope Generation) code, part of the SCALE nuclear systems modeling and simulation package, has been integrated into CRISP. Measurement data are collected in an unattended mode by RADAR and then processed by CRISP, which outputs, for each fuel assembly, the measured gamma and neutron count rates. Simultaneously, ORIGEN performs burn-up calculations based on operator declarations previously entered into CRISP and calculates the expected neutron and gamma count rates for each assembly. These calculations also used response functions, developed using Monte Carlo modeling, to account for the detection probabilities of both neutron and photon particles that originated in each fuel pin. Finally, CRISP correlates and compares the expected (calculated) gamma

  17. The role of psychological determinants and demographic factors in consumer demand for farm-to-fork traceability systems.

    Science.gov (United States)

    Myae, Aye Chan; Goddard, Ellen; Aubeeluck, Ashwina

    2011-01-01

    Traceability systems are an important tool (1) for tracking, monitoring, and managing product flows through the supply chain for better efficiency and profitability of suppliers, and (2) to improve consumer confidence in the face of serious food safety incidents. After the global bovine spongiform encephalopathy (BSE) crisis affected producers, consumers, trade, and the health status of animals and humans, new systems to help confirm the status of cattle products along the supply chain from farm to fork were implemented in many countries (Trautman et al. 2008 ). In this study, people's overall food safety beliefs are explored with the main objective of measuring the link between their food safety beliefs and their attitudes toward traceability. A comparison is made among English-speaking Canadians, French-speaking Canadians, and Japanese consumers. In the study, an Internet-based survey was used to collect data from nationally representative samples of the population in Canada-English (1275), Canada-French (343), and Japanese (1940) in the summer of 2009. Respondents' interests in traceability systems are clearly linked to their sense that the industry is primarily responsible for any food safety outbreaks. Moreover, it is clear that certain segments of the population in all samples feel strongly about the importance of farm to fork traceability in beef; thus, policymakers may wish to consider extending traceability beyond the point of slaughter as a way of encouraging beef sales in Canada.

  18. Quality Assurance Plan for the Upper East Fork Poplar Creek Characterization Area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-12-01

    This quality assurance plan summarizes requirements for conducting work on the Upper East 9 Fork Poplar Creek (UEFPC) Characterization Area (CA). The reader is referred to the Expanded Task Work Agreement for Upper East Fork Poplar Creek Characterization Area, Remedial Investigation/Feasibility Study (RI/FS) for details regarding the activities, roles, and responsibilities summarized here. UEFPC is designated a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site and thus requires a remedial investigation (RI) and a feasibility study (FS). The RI objectives are to evaluate the nature and extent of known and suspected contaminates, to provide data to perform baseline ecological and human health risk assessments, and to support development and evaluation of remedial alternatives for the FS,. Existing data will be used as much as possible. Additional sampling may be required to fill data gaps. The goal of the RI is to prioritize the major sources of contaminants to exit pathways and to understand their characteristics for risk characterization and development of remedial alternatives. The FS objectives are to investigate technologies and develop and evaluate alternatives based on 2031 CERCLA guidance

  19. Mutation spectrum of fork-head transcriptional factor gene (FOXL2) in Indian Blepharophimosis Ptosis Epicanthus Inversus Syndrome (BPES) patients.

    Science.gov (United States)

    Kaur, Inderjeet; Hussain, Avid; Naik, Milind N; Murthy, Ramesh; Honavar, Santosh G

    2011-06-01

    The fork-head transcription factor gene (FOXL2) gene has been implicated in Blepharophimosis Ptosis Epicanthus Inversus Syndrome (BPES) type I and type II. The authors aimed to evaluate the involvement of FOXL2 in familial and sporadic cases of BPES in an Indian cohort. The present cohort comprised clinically well-characterised BPES cases that included six affected families, two sporadic cases and 60 unaffected normal controls. The 5' untranslated and coding region of FOXL2 was screened by resequencing and confirmed by restriction digestion. Further, genotype-phenotype correlations were done to understand the implications of the observed mutation. Six mutations were observed in eight cases (87.5%). These included a novel deletion (c.860delC), three previously reported duplications (c.663-692dup 30, c.672-701dup30 and c.843-859dup17), a frame shift (c.804dupC) and a homozygous missense mutation (p.E69K). The p.E69k mutation was seen in both heterozygous and homozygous form in a large four-generational family, and disease severity was found to be directly linked to the allelic dosage. Two SNPs (c.501C→T, c.536C→G) were also noted. An unusual coexistence of polycystic ovarian disease (PCOD) with BPES was also seen in one of the families. Mutations in the region downstream of the fork-head domain were predominantly responsible for BPES among Indian patients.

  20. Best management practices plan for the Lower East Fork Poplar Creek remedial action project, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-08-01

    The U.S. Department of Energy (DOE) has three major operating facilities on the DOE Oak Ridge Reservation (ORR) in Oak Ridge, Tennessee: the Oak Ridge Y-12 Plant, the K-25 Site, and the Oak Ridge National Laboratory (ORNL) managed by Lockheed Martin Environmental Research Corporation. All facilities are managed by Lockheed Martin Energy Systems, Incorporated (Energy Systems) for the DOE. The Y-12 Plant is adjacent to the city of Oak Ridge and is also upstream from Oak Ridge along East Fork Poplar Creek. The portion of the creek downstream from the Y-12 Plant is Lower East Fork Poplar Creek (LEFPC). This project will remove mercury-contaminated soils from the LEFPC floodplain, transport the soils to Industrial Landfill V (ILF-V), and restore any affected areas. This project contains areas that were designated in 1989 as a Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) site. The site includes DOE property and portions of commercial, residential, agricultural, and miscellaneous areas within the city of Oak Ridge

  1. A High Sensitivity Preamplifier for Quartz Tuning Forks in QEPAS (Quartz Enhanced PhotoAcoustic Spectroscopy Applications

    Directory of Open Access Journals (Sweden)

    Tomasz Starecki

    2017-11-01

    Full Text Available All the preamplifiers dedicated for Quartz Enhanced PhotoAcoustic Spectroscopy (QEPAS applications that have so far been reported in the literature have been based on operational amplifiers working in transimpedance configurations. Taking into consideration that QEPAS sensors are based on quartz tuning forks, and that quartz has a relatively high voltage constant and relatively low charge constant, it seems that a transimpedance amplifier is not an optimal solution. This paper describes the design of a quartz QEPAS sensor preamplifier, implemented with voltage amplifier configuration. Discussion of an electrical model of the circuit and preliminary measurements are presented. Both theoretical analysis and experiments show that use of the voltage configuration allows for a substantial increase of the output signal in comparison to the transimpedance circuit with the same tuning fork working in identical conditions. Assuming that the sensitivity of the QEPAS technique depends directly on the properties of the preamplifier, use of the voltage amplifier configuration should result in an increase of QEPAS sensitivity by one to two orders of magnitude.

  2. A High Sensitivity Preamplifier for Quartz Tuning Forks in QEPAS (Quartz Enhanced PhotoAcoustic Spectroscopy) Applications.

    Science.gov (United States)

    Starecki, Tomasz; Wieczorek, Piotr Z

    2017-11-03

    All the preamplifiers dedicated for Quartz Enhanced PhotoAcoustic Spectroscopy (QEPAS) applications that have so far been reported in the literature have been based on operational amplifiers working in transimpedance configurations. Taking into consideration that QEPAS sensors are based on quartz tuning forks, and that quartz has a relatively high voltage constant and relatively low charge constant, it seems that a transimpedance amplifier is not an optimal solution. This paper describes the design of a quartz QEPAS sensor preamplifier, implemented with voltage amplifier configuration. Discussion of an electrical model of the circuit and preliminary measurements are presented. Both theoretical analysis and experiments show that use of the voltage configuration allows for a substantial increase of the output signal in comparison to the transimpedance circuit with the same tuning fork working in identical conditions. Assuming that the sensitivity of the QEPAS technique depends directly on the properties of the preamplifier, use of the voltage amplifier configuration should result in an increase of QEPAS sensitivity by one to two orders of magnitude.

  3. Helicase and Polymerase Move Together Close to the Fork Junction and Copy DNA in One-Nucleotide Steps

    Directory of Open Access Journals (Sweden)

    Manjula Pandey

    2014-03-01

    Full Text Available By simultaneously measuring DNA synthesis and dNTP hydrolysis, we show that T7 DNA polymerase and T7 gp4 helicase move in sync during leading-strand synthesis, taking one-nucleotide steps and hydrolyzing one dNTP per base-pair unwound/copied. The cooperative catalysis enables the helicase and polymerase to move at a uniformly fast rate without guanine:cytosine (GC dependency or idling with futile NTP hydrolysis. We show that the helicase and polymerase are located close to the replication fork junction. This architecture enables the polymerase to use its strand-displacement synthesis to increase the unwinding rate, whereas the helicase aids this process by translocating along single-stranded DNA and trapping the unwound bases. Thus, in contrast to the helicase-only unwinding model, our results suggest a model in which the helicase and polymerase are moving in one-nucleotide steps, DNA synthesis drives fork unwinding, and a role of the helicase is to trap the unwound bases and prevent DNA reannealing.

  4. DNA polymerase η modulates replication fork progression and DNA damage responses in platinum-treated human cells

    Science.gov (United States)

    Sokol, Anna M.; Cruet-Hennequart, Séverine; Pasero, Philippe; Carty, Michael P.

    2013-11-01

    Human cells lacking DNA polymerase η (polη) are sensitive to platinum-based cancer chemotherapeutic agents. Using DNA combing to directly investigate the role of polη in bypass of platinum-induced DNA lesions in vivo, we demonstrate that nascent DNA strands are up to 39% shorter in human cells lacking polη than in cells expressing polη. This provides the first direct evidence that polη modulates replication fork progression in vivo following cisplatin and carboplatin treatment. Severe replication inhibition in individual platinum-treated polη-deficient cells correlates with enhanced phosphorylation of the RPA2 subunit of replication protein A on serines 4 and 8, as determined using EdU labelling and immunofluorescence, consistent with formation of DNA strand breaks at arrested forks in the absence of polη. Polη-mediated bypass of platinum-induced DNA lesions may therefore represent one mechanism by which cancer cells can tolerate platinum-based chemotherapy.

  5. Role of river flow and sediment mobilization in riparian alder establishment along a bedrock-gravel river, South Fork Eel River, California

    Science.gov (United States)

    Jablkowski, P.; Johnson, E. A.; Martin, Y. E.

    2017-10-01

    Climatic, hydraulics, hydrologic, and fluvial geomorphic processes are the main drivers of riparian white alder (Alnus rhombifolia Nutt.) distribution in northern California. The Mediterranean climate and canyon bound, bedrock-gravel morphology of the South Fork Eel have a distinct effect on these processes. White alder seeds are preferentially deposited on river bars where river hydraulics create eddies coinciding with the downstream part of riffles and the upstream part of pools. Seeds are generally deposited below bankfull elevations by the descending hydrograph during the spring season in this Mediterranean climate. For successful germination and establishment, the seeds must be deposited at a location such that they are not remobilized by late spring flows. The summer establishment period is defined from the date of seed deposition and germination to the fall/winter date of river sediment mobilization. Seedling root growth rate decreases exponentially with decreasing water potential. However, seedlings are shown not to be generally limited by water availability at the elevations they are most commonly deposited. The establishment of white alder seedlings following the first summer will therefore depend on their ability to resist fall/winter high flows. The method proposed here compares the predicted rooting depth to predicted sediment scour rates. The length of the establishment period rather than water availability determines final seedling rooting depth. Over the past 40 years, very few years had establishment periods that were long enough or had fast enough alder growth rates to survive winter floods that often scour deeper than the total root length. The low survival of seedlings in the first autumn season following germination is believed to be a principal reason for the missing age classes often found in alder distributions along rivers.

  6. Sampling and analysis plan for treatment water and creek water for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1996-04-01

    This document provides the Environmental Restoration Program with information about the methodology, organizational structure, quality assurance and health and safety practices to be employed during the water sampling and analysis activities associated with the remediation of the Lower East Fork Poplar Creek Operable Unit during remediation of the National Oceanic and Atmospheric Administration and Bruner sites

  7. Sampling and analysis plan for treatment water and creek water for the Lower East Fork Poplar Creek Operable Unit, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-04-01

    This document provides the Environmental Restoration Program with information about the methodology, organizational structure, quality assurance and health and safety practices to be employed during the water sampling and analysis activities associated with the remediation of the Lower East Fork Poplar Creek Operable Unit during remediation of the National Oceanic and Atmospheric Administration and Bruner sites.

  8. Habitat selection influences sex distribution, morphology, tissue biochemistry, and parasite load of juvenile coho salmon in the West Fork Smith River, Oregon

    Science.gov (United States)

    Given the strong influence of water temperature on salmonid physiology and behavior, in the summers of 2004 and 2005 we studied juvenile male and female coho salmon Oncorhynchus kisutch in two reaches of Oregon’s West Fork Smith River with different thermal profiles. Our goals we...

  9. Status of the dirty darter, Etheostoma olivaceum, and bluemask darter, Etheostoma (Doration)sp., with notes on fishes of the Caney Fork River system, Tennessee

    International Nuclear Information System (INIS)

    Layman, S.R.; Simons, A.M.; Wood, R.M.

    1993-01-01

    Seventy-six localities were sampled in the Caney Fork River system and adjacent Cumberland River tributaries. Etheostoma olivaceum was found in small creeks from nine tributaries of lower Caney Fork River and three tributaries of the Cumberland River in the Nashville Basin physiographic province. The species was most abundant around slab rocks and rubble over bedrock in slow to moderate current. Etheostoma olivaceum was common throughout its small range; however, given widespread habitat degradation from agriculture, the species should retain its open-quotes deemed in need of managementclose quotes status in Tennessee. The bluemask darter, Etheostoma (Doration) sp., was collected in slow to moderate current over sand and gravel in Collins River, Rocky River, Cane Creek, and Caney Fork River. All four populations were isolated upstream of Great Falls Reservoir in the Highland Rim physiographic province. The species was found in a 37-km reach of Collins River but was restricted to reaches of 0.2 to 4.3 km in the other three streams. Threats to the species include pesticides from plant nurseries, siltation, gravel dredging, and acid mine drainage. The authors recommend that the bluemask darter be listed as state and federally protected. Two new records were established for the rare Barrens darter, Etheostoma forbesi, in lower Collins River and Barren Fork River, and eight previously unknown records of the species were identified from older museum collections. 21 refs., 1 fig., 1 tab

  10. Mercury Contributions from Flint Creek and other Tributaries to the Upper Clark Fork River in Northwestern Montana

    Science.gov (United States)

    Langner, H.; Young, M.; Staats, M. F.

    2013-12-01

    Methylmercury contamination in biota is a major factor diminishing the environmental quality of the Upper Clark Fork River (CFR), e.g. by triggering human consumption limits of fish. The CFR is subject to one of the largest Superfund cleanup projects in the US, but remediation and restoration is currently focused exclusively on other mining-related contaminants (As, Cu, Zn, Pb, Cd), which may be counterproductive with respect to the bio-availability of mercury, for example by creation of wetlands along mercury-contaminated reaches of the river. The identification and elimination of Hg sources is an essential step toward reducing the methylmercury exposure in the biota of the CFR watershed because a strong correlation exists between total mercury levels in river sediment and methylmercury levels in aquatic life. We analyzed duplicate samples from the top sediment layer of the main stem and significant tributaries to the Clark Fork River along a 240 km reach between Butte, MT and downstream of the Missoula Valley. Mercury concentrations were 1.3 × 1.6 (mean × SD, n = 35) in the main stem. Concentrations in tributaries varied widely (0.02 to 85 mg/kg) and seemed only loosely related to the number of historic precious metal mines in the watershed. In the upper reach of the CFR, elevated Hg levels are likely caused by residual contaminated sediments in the flood plain. Levels tend to decrease downstream until Drummond, MT, where Flint Creek contributes a significant amount of mercury, causing Hg levels in the main stem CFR to increase from 0.7 to 4 mg/kg. Levels continue to decrease downstream. Flint Creek is the single largest contributor of Hg to the CFR. Detailed sampling of the main stem Flint Creek and tributaries (26 sites) showed extremely high levels in two tributaries (22 to 85 mg/kg) where historic milling operations were located. Elimination of these point sources may be accomplished comparatively economically and may significantly reduce mercury levels in

  11. Historical perspectives on channel pattern in the Clark Fork River, Montana and implications for post-dam removal restoration

    Science.gov (United States)

    Woelfle-Erskine, C. A.; Wilcox, A. C.

    2009-12-01

    Active restoration approaches such as channel reconstruction have moved beyond the realm of small streams and are being applied to larger rivers. Uncertainties arising from limited knowledge, fluvial and ecosystem variability, and contaminants are especially significant in restoration of large rivers, where project costs and the social, infrastructural, and ecological costs of failure are high. We use the case of Milltown Dam removal on the Clark Fork River, Montana and subsequent channel reconstruction in the former reservoir to examine the use of historical research and uncertainty analysis in river restoration. At a cost of approximately $120 million, the Milltown Dam removal involves the mechanical removal of approximately 2 million cubic meters of sediments contaminated by upstream mining, followed by restoration of the former reservoir reach in which a single-thread meandering channel is being constructed. Historical maps, surveys, photographs, and accounts suggest a conceptual model of a multi-thread, anastomosing river in the reach targeted for channel reconstruction, upstream of the confluence of the Clark Fork and Blackfoot Rivers. We supplemented historical research with analysis of aerial photographs, topographic data, and USGS stage-discharge measurements in a lotic but reservoir-influenced reach of the Clark Fork River within our study area to estimate avulsion frequency (0.8 avulsions/year over a 70-year period) and average rates of lateral migration and aggradation. These were used to calculate the mobility number, a dimensionless relationship between channel filling and lateral migration timescales that can be used to predict whether a river’s planform is single or multi-threaded. The mobility number within our study reach ranged from 0.6 (multi-thread channel) to 1.7 (transitional channel). We predict that, in the absence of active channel reconstruction, the post-dam channel pattern would evolve to one that alternates between single and multi

  12. Environmental restoration of mercury contamination of East Fork Poplar Creek at the Department of Energy's Oak Ridge, Tennessee, reservation

    International Nuclear Information System (INIS)

    Page, D.G.

    1995-01-01

    During the open-quotes Cold Warclose quotes era, approximately 239,000 pounds of mercury were released from the Y-12 Nuclear Weapons Plant to the East Fork Poplar Creek watershed. As a result, approximately 75 tons of the contaminant resides within the floodplain soils beyond the confines of the DOE reservation, a Federal Superfund Site. The EFPC watershed encompasses multiple land uses whose ownership varies from private citizens, municipal government, and federal government. DOE, in cooperation with the State of Tennessee and EPA, proposes to clean up the contamination to a risk based standard of 400 ppm. This level has been determined to be protective of human health and the environment. The remedial process and development of the remedial alternative are the result of close interagency cooperation between the State, EPA, U.S. Fish ampersand Wildlife Service, and the Army Corps of Engineers. This case study outlines that process

  13. DNA Replication Control During Drosophila Development: Insights into the Onset of S Phase, Replication Initiation, and Fork Progression

    Science.gov (United States)

    Hua, Brian L.; Orr-Weaver, Terry L.

    2017-01-01

    Proper control of DNA replication is critical to ensure genomic integrity during cell proliferation. In addition, differential regulation of the DNA replication program during development can change gene copy number to influence cell size and gene expression. Drosophila melanogaster serves as a powerful organism to study the developmental control of DNA replication in various cell cycle contexts in a variety of differentiated cell and tissue types. Additionally, Drosophila has provided several developmentally regulated replication models to dissect the molecular mechanisms that underlie replication-based copy number changes in the genome, which include differential underreplication and gene amplification. Here, we review key findings and our current understanding of the developmental control of DNA replication in the contexts of the archetypal replication program as well as of underreplication and differential gene amplification. We focus on the use of these latter two replication systems to delineate many of the molecular mechanisms that underlie the developmental control of replication initiation and fork elongation. PMID:28874453

  14. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere.

  15. Determination of the static spring constant of electrically-driven quartz tuning forks with two freely oscillating prongs

    International Nuclear Information System (INIS)

    González, Laura; Oria, Roger; Botaya, Luis; Puig-Vidal, Manel; Otero, Jorge

    2015-01-01

    Quartz tuning forks have become popular in nanotechnology applications, especially as sensors for scanning probe microscopy. The sensor’s spring constant and the oscillation amplitude are required parameters to evaluate the tip-sample forces; however, there is certain controversy within the research community as to how to arrive at a value for the static spring constant of the device when working in shear mode. Here, we present two different methods based on finite element simulations, to determine the value of the spring constant of the sensors: the amplitude and Cleveland methods. The results obtained using these methods are compared to those using the geometrical method, and show that the latter overestimates the spring constant of the device. (paper)

  16. Final report from VFL technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils

    International Nuclear Information System (INIS)

    1994-09-01

    The Lower East Fork Poplar Creek (LEFPC) extends fourteen (14) miles through Oak Ridge, TN. The Creek sediments and surrounding floodplain soils are contaminated with mercury compounds. This project involved a comprehensive pilot demonstration on thermal desorption of these soils to validate the feasibility of the remedial technology which had been identified in previous studies. Thermal desorption is a technology that utilizes heating or drying of soils to induce volatilization of contaminants. These contaminants are then vaporized and either incinerated or condensed in the second stage of desorption. Mercury (Hg), which was the principal contaminate of concern, was collected by condensers in a vapor collection system. This type of system insured that the toxic mercury vapors did not escape to the atmosphere

  17. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant

    International Nuclear Information System (INIS)

    1992-08-01

    This report contains an evaluation of the groundwater quality data obtained during the 1991 calendar year at several hazardous and non-hazardous waste management facilities and underground storage tanks (USTs) associated with the US Department of Energy Y-12 Plant. These sites are within the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three regimes defined for the purposes of groundwater and surfacewater quality monitoring. Section 2.0 of this report contains background information regarding groundwater monitoring at the waste-management sites and USTs located in the UEFPCHR. An overview of the hydrogeologic system in the UEFPCHR is provided in Section 3.0. A discussion of the interpretive assumptions used in evaluating the 1991 assessment data, and detailed descriptions of groundwater quality are presented in Section 4.0. Findings of the 1991 monitoring program are summarized in Section 5.0. Proposed modifications to the groundwater quality monitoring program in the UEFPCHR are presented

  18. Phase 2 confirmatory sampling data report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    A Remedial Investigation of East Fork Poplar Creek (EFPC) concluded that mercury is the principal contaminant of concern in the EFPC floodplain. The highest concentrations of mercury were found to be in a visually distinct black layer of soil that typically lies 15 to 30 cm (6 to 12 in.) below the surface. Mercury contamination was found to be situated in distinct areas along the floodplain, and generally at depths > 20 cm (8 in.) below the surface. In accordance with Comprehensive, Environmental Response, Compensation, and Liability Act (CERCLA), a feasibility study was prepared to assess alternatives for remediation, and a proposed plan was issued to the public in which a preferred alternative was identified. In response to public input, the plan was modified and US Department of Energy (DOE) issued a Record of Decision in 1995 committing to excavating all soil in the EFPC floodplain exceeding a concentration of 400 parts per million (ppm) of mercury. The Lower East Fork Poplar Creek (LEFPC) remedial action (RA) focuses on the stretch of EFPC flowing from Lake Reality at the Y-12 Plant, through the city of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation (ORR) and its associated floodplain. Specific areas were identified that required remediation at the National Oceanographic and Atmospheric Administration (NOAA) Site along Illinois Avenue and at the Bruner Site along the Oak Ridge Turnpike. The RA was conducted in two separate phases. Phase 2, conducted from February to October 1997, completed the remediation efforts at the NOAA facility and fully remediated the Bruner Site. During both phases, data were collected to show that the remedial efforts performed at the NOAA and Bruner sites were successful in implementing the Record of Decision and had no adverse impact on the creek water quality or the city of Oak Ridge publicly owned treatment works.

  19. Phase II confirmatory sampling data report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1998-01-01

    A Remedial Investigation of East Fork Poplar Creek (EFPC) concluded that mercury is the principal contaminant of concern in the EFPC floodplain. The highest concentrations of mercury were found to be in a visually distinct black layer of soil that typically lies 15 to 30 cm (6 to 12 in.) below the surface. Mercury contamination was found to be situated in distinct areas along the floodplain, and generally at depths > 20 cm (8 in.) below the surface. In accordance with Comprehensive, Environmental Response, Compensation, and Liability Act (CERCLA), a feasibility study was prepared to assess alternatives for remediation, and a proposed plan was issued to the public in which a preferred alternative was identified. In response to public input, the plan was modified and US Department of Energy (DOE) issued a Record of Decision in 1995 committing to excavating all soil in the EFPC floodplain exceeding a concentration of 400 parts per million (ppm) of mercury. The Lower East Fork Poplar Creek (LEFPC) remedial action (RA) focuses on the stretch of EFPC flowing from Lake Reality at the Y-12 Plant, through the city of Oak Ridge, to Poplar Creek on the Oak Ridge Reservation (ORR) and its associated floodplain. Specific areas were identified that required remediation at the National Oceanographic and Atmospheric Administration (NOAA) Site along Illinois Avenue and at the Bruner Site along the Oak Ridge Turnpike. The RA was conducted in two separate phases. Phase 2, conducted from February to October 1997, completed the remediation efforts at the NOAA facility and fully remediated the Bruner Site. During both phases, data were collected to show that the remedial efforts performed at the NOAA and Bruner sites were successful in implementing the Record of Decision and had no adverse impact on the creek water quality or the city of Oak Ridge publicly owned treatment works

  20. Final report from VFL Technologies for the pilot-scale thermal treatment of Lower East Fork Poplar Creek floodplain soils. LEFPC appendices. Volume 6. Appendix VI-X

    International Nuclear Information System (INIS)

    1994-09-01

    This final report from VFL Technologies for the pilot-scale thermal treatment of lower East Fork Poplar Creek floodplain soils dated September 1994 contains LEFPC Appendices, Volume 6, Appendix VI - X. These appendices cover the following areas: chain of custody, miscellaneous process calculations (residence time and orifice plate calculations), waste management (mercury and radiation confirmatory testing before and after final verification run), health and safety (training, respirator fit test and radiation work permits), and transportation (soil receipt documentation)

  1. Mercury and selenium concentrations in biofilm, macroinvertebrates, and fish collected in the Yankee Fork of the Salmon River, Idaho, USA, and their potential effects on fish health

    Science.gov (United States)

    Rhea, Darren T.; Farag, Aïda M.; Harper, David D.; McConnell, Elizabeth; Brumbaugh, William G.

    2013-01-01

    The Yankee Fork is a large tributary of the Salmon River located in central Idaho, USA, with an extensive history of placer and dredge-mining activities. Concentrations of selenium (Se) and mercury (Hg) in various aquatic trophic levels were measured in the Yankee Fork during 2001 and 2002. Various measurements of fish health were also performed. Sites included four on the mainstem of the Yankee Fork and two off-channel sites in partially reclaimed dredge pools used as rearing habitat for cultured salmonid eggs and fry. Hg concentrations in whole mountain whitefish and shorthead sculpin ranged from 0.28 to 0.56 μg/g dry weight (dw), concentrations that are generally less than those reported to have significant impacts on fish. Biofilm and invertebrates ranged from 0.05 to 0.43 μg Hg/g dw. Se concentrations measured in biota samples from the Yankee Fork were greater than many representative samples collected in the Snake and Columbia watersheds and often exceeded literature-based toxic thresholds. Biofilm and invertebrates ranged from 0.58 to 4.66 μg Se/g dw. Whole fish ranged from 3.92 to 7.10 μg Se/g dw, and gonads ranged from 6.91 to 31.84 μg Se/g dw. Whole-body Se concentrations exceeded reported toxicological thresholds at three of four sites and concentrations in liver samples were mostly greater than concentrations shown to have negative impacts on fish health. Histological examinations performed during this study noted liver abnormalities, especially in shorthead sculpin, a bottom-dwelling species.

  2. An Assessment of health risk associated with mercury in soil and sediment from East Fork Poplar Creek, Oak Ridge, Tennessee. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Revis, N.; Holdsworth, G.; Bingham, G.; King, A.; Elmore, J.

    1989-04-01

    This report presents results from a study conducted to determine the toxicity of Mercury in soils sediments samples. Mice were fed via diet, soils and sediment, from various locations along the East Fork Poplar creek. Tissue distribution of pollutants was determined at various intervals. The tissue level relative to toxicity was used to determine the effect of a complex matrix on the gastrointestinal absorption and tissue distribution of the pollutants (other pollutants included cadmium and selenium).

  3. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    2010-12-01

    Full Text Available We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome.

  4. Water-quality, bed-sediment, and biological data (October 2015 through September 2016) and statistical summaries of data for streams in the Clark Fork Basin, Montana

    Science.gov (United States)

    Dodge, Kent A.; Hornberger, Michelle I.; Turner, Matthew A.

    2018-03-30

    Water, bed sediment, and biota were sampled in selected streams from Butte to near Missoula, Montana, as part of a monitoring program in the upper Clark Fork Basin of western Montana. The sampling program was led by the U.S. Geological Survey, in cooperation with the U.S. Environmental Protection Agency, to characterize aquatic resources in the Clark Fork Basin, with emphasis on trace elements associated with historic mining and smelting activities. Sampling sites were on the Clark Fork and selected tributaries. Water samples were collected periodically at 20 sites from October 2015 through September 2016. Bed-sediment and biota samples were collected once at 13 sites during August 2016.This report presents the analytical results and quality-assurance data for water-quality, bed-sediment, and biota samples collected at sites from October 2015 through September 2016. Water-quality data include concentrations of selected major ions, trace elements, and suspended sediment. Samples for analysis of turbidity were collected at 13 sites, whereas samples for analysis of dissolved organic carbon were collected at 10 sites. In addition, samples for analysis of nitrogen (nitrate plus nitrite) were collected at two sites. Daily values of mean suspended-sediment concentration and suspended-sediment discharge were determined for three sites. Seasonal daily values of turbidity were determined for five sites. Bed-sediment data include trace-element concentrations in the fine-grained (less than 0.063 millimeter) fraction. Biological data include trace-element concentrations in whole-body tissue of aquatic benthic insects. Statistical summaries of water-quality, bed-sediment, and biological data for sites in the upper Clark Fork Basin are provided for the period of record.

  5. Lingering single-strand breaks trigger Rad51-independent homology-directed repair of collapsed replication forks in the polynucleotide kinase/phosphatase mutant of fission yeast.

    Directory of Open Access Journals (Sweden)

    Arancha Sanchez

    2017-09-01

    Full Text Available The DNA repair enzyme polynucleotide kinase/phosphatase (PNKP protects genome integrity by restoring ligatable 5'-phosphate and 3'-hydroxyl termini at single-strand breaks (SSBs. In humans, PNKP mutations underlie the neurological disease known as MCSZ, but these individuals are not predisposed for cancer, implying effective alternative repair pathways in dividing cells. Homology-directed repair (HDR of collapsed replication forks was proposed to repair SSBs in PNKP-deficient cells, but the critical HDR protein Rad51 is not required in PNKP-null (pnk1Δ cells of Schizosaccharomyces pombe. Here, we report that pnk1Δ cells have enhanced requirements for Rad3 (ATR/Mec1 and Chk1 checkpoint kinases, and the multi-BRCT domain protein Brc1 that binds phospho-histone H2A (γH2A at damaged replication forks. The viability of pnk1Δ cells depends on Mre11 and Ctp1 (CtIP/Sae2 double-strand break (DSB resection proteins, Rad52 DNA strand annealing protein, Mus81-Eme1 Holliday junction resolvase, and Rqh1 (BLM/WRN/Sgs1 DNA helicase. Coupled with increased sister chromatid recombination and Rad52 repair foci in pnk1Δ cells, these findings indicate that lingering SSBs in pnk1Δ cells trigger Rad51-independent homology-directed repair of collapsed replication forks. From these data, we propose models for HDR-mediated tolerance of persistent SSBs with 3' phosphate in pnk1Δ cells.

  6. Acute Smc5/6 depletion reveals its primary role in rDNA replication by restraining recombination at fork pausing sites.

    Directory of Open Access Journals (Sweden)

    Xiao P Peng

    2018-01-01

    Full Text Available Smc5/6, a member of the conserved SMC family of complexes, is essential for growth in most organisms. Its exact functions in a mitotic cell cycle are controversial, as chronic Smc5/6 loss-of-function alleles produce varying phenotypes. To circumvent this issue, we acutely depleted Smc5/6 in budding yeast and determined the first cell cycle consequences of Smc5/6 removal. We found a striking primary defect in replication of the ribosomal DNA (rDNA array. Each rDNA repeat contains a programmed replication fork barrier (RFB established by the Fob1 protein. Fob1 removal improves rDNA replication in Smc5/6 depleted cells, implicating Smc5/6 in the management of programmed fork pausing. A similar improvement is achieved by removing the DNA helicase Mph1 whose recombinogenic activity can be inhibited by Smc5/6 under DNA damage conditions. DNA 2D gel analyses further show that Smc5/6 loss increases recombination structures at RFB regions; moreover, mph1∆ and fob1∆ similarly reduce this accumulation. These findings point to an important mitotic role for Smc5/6 in restraining recombination events when protein barriers in rDNA stall replication forks. As rDNA maintenance influences multiple essential cellular processes, Smc5/6 likely links rDNA stability to overall mitotic growth.

  7. Electrospun single crystalline fork-like K2V8O21 as high-performance cathode materials for lithium-ion batteries

    Science.gov (United States)

    Hao, Pengfei; Zhu, Ting; Su, Qiong; Lin, Jiande; Cui, Rong; Cao, Xinxin; Wang, Yaping; Pan, Anqiang

    2018-06-01

    Single crystalline fork-like potassium vanadate (K2V8O21) has been successfully prepared through electrospinning combined with a subsequent annealing process. The as-obtained K2V8O21 forks show a unique layer-by-layer stacked structure with conductive carbon. When used as cathode materials for lithium-ion batteries, the as-prepared fork-like materials exhibit high specific discharge capacity and excellent cyclic stability. High specific discharge capacity of 200.2 mA h g-1 and 131.5 mA h g-1 can be delivered at the current densities of 50 mA g-1 and 500 mA g-1, respectively. Furthermore, the K2V8O21 electrodes exhibit excellent long-term cycling stability that maintain a capacity of 108.3 mA h g-1 after 300 cycles at 500 mA g-1 with a fading rate of only 0.054% per cycle, revealing their potential applications in next generation high-performance lithium-ion batteries.

  8. Effects of catastrophic floods and debris flows on the sediment retention structure, North Fork Toutle River, Washington

    Science.gov (United States)

    Denlinger, Roger P.

    2012-01-01

    The eruption of Mount St. Helens in 1980 produced a debris avalanche that flowed down the upper reaches of the North Fork Toutle River in southwestern Washington, clogging this drainage with sediment. In response to continuous anomalously high sediment flux into the Toutle and Cowlitz Rivers resulting from this avalanche and associated debris flows, the U.S. Army Corps of Engineers completed a Sediment Retention Structure (SRS) on the North Fork Toutle River in May 1989. For one decade, the SRS effectively blocked most of the sediment transport down the Toutle River. In 1999, the sediment level behind the SRS reached the elevation of the spillway base. Since then, a higher percentage of sediment has been passing the SRS and increasing the flood risk in the Cowlitz River. Currently (2012), the dam is filling with sediment at a rate that cannot be sustained for its original design life, and the U.S. Army Corps of Engineers is concerned with the current ability of the SRS to manage floods. This report presents an assessment of the ability of the dam to pass large flows from three types of scenarios (it is assumed that no damage to the spillway will occur). These scenarios are (1) a failure of the debris-avalanche blockage forming Castle Lake that produces a dambreak flood, (2) a debris flow from failure of that blockage, or (3) a debris flow originating in the crater of Mount St. Helens. In each case, the flows are routed down the Toutle River and through the SRS using numerical models on a gridded domain produced from a digital elevation model constructed with existing topography and dam infrastructure. The results of these simulations show that a structurally sound spillway is capable of passing large floods without risk of overtopping the crest of the dam. In addition, large debris flows originating from Castle Lake or the crater of Mount St. Helens never reach the SRS. Instead, debris flows fill the braided channels upstream of the dam and reduce its storage

  9. The use of computed neutron coincidence counting with time interval analysis for the analysis of Fork-measurements on a fresh MOX-LWR fuel assembly under water

    Energy Technology Data Exchange (ETDEWEB)

    Baeten, P.; Bruggeman, M.; Carchon, R

    1998-06-01

    The objective of this study was to investigate the influence of different important parameters on measurement results for various fork-detectors. Computed Neutron Coincidence Counting (CNCC) with Time Interval Analysis (TIA) was used for this study. The performance of the electronics for the different fork-detectors was studied by investigating the deadtime perturbed zone of the Rossi-alpha distribution in TIA. The measurement revealed anomalies in the performance of the electronics of the IAEA BWR and LANL fork-detector. The IAEA PWR fork-detector functioned well and the deadtime parameter was calculated. The optimal setting for the pre delay was investigated and it was found that a pre delay of 10 micro seconds should be considered as an optimum between excluding from analysis data in the deadtime perturbed zone and keeping a high signal-to-noise ratio. For the shift register electronics used with the fork-detectors, a pre delay of only 4.5 micro seconds was used. The study of the pre delay and the deadtime showed that the calculated triples-rate is strongly dependent on these parameters. An accurate determination of the triple-rate in this type of measurements has proven to be quite difficult and requires proper operation of the electronics, a correct pre delay and an accurate deadtime correction formalism. By varying the boron concentration in water, the change of the decay time of the Rossi-alpha distribution was clearly observed. This change is due to the variation of the thermal multiplication. The variation of this decay time with the boron concentration proves that Boehnel's model for fast neutron multiplication is not valid under these measurement conditions and that a model for fast and thermal multiplication should be used in order to obtain unbiased measurement results. CNCC with TIA has proved to be a valuable tool in which parameter settings can be varied a posterori and the optimal setting can be determined for each measurement. Moreover, the

  10. The use of computed neutron coincidence counting with time interval analysis for the analysis of Fork-measurements on a fresh MOX-LWR fuel assembly under water

    International Nuclear Information System (INIS)

    Baeten, P.; Bruggeman, M.; Carchon, R.

    1998-06-01

    The objective of this study was to investigate the influence of different important parameters on measurement results for various fork-detectors. Computed Neutron Coincidence Counting (CNCC) with Time Interval Analysis (TIA) was used for this study. The performance of the electronics for the different fork-detectors was studied by investigating the deadtime perturbed zone of the Rossi-alpha distribution in TIA. The measurement revealed anomalies in the performance of the electronics of the IAEA BWR and LANL fork-detector. The IAEA PWR fork-detector functioned well and the deadtime parameter was calculated. The optimal setting for the pre delay was investigated and it was found that a pre delay of 10 micro seconds should be considered as an optimum between excluding from analysis data in the deadtime perturbed zone and keeping a high signal-to-noise ratio. For the shift register electronics used with the fork-detectors, a pre delay of only 4.5 micro seconds was used. The study of the pre delay and the deadtime showed that the calculated triples-rate is strongly dependent on these parameters. An accurate determination of the triple-rate in this type of measurements has proven to be quite difficult and requires proper operation of the electronics, a correct pre delay and an accurate deadtime correction formalism. By varying the boron concentration in water, the change of the decay time of the Rossi-alpha distribution was clearly observed. This change is due to the variation of the thermal multiplication. The variation of this decay time with the boron concentration proves that Boehnel's model for fast neutron multiplication is not valid under these measurement conditions and that a model for fast and thermal multiplication should be used in order to obtain unbiased measurement results. CNCC with TIA has proved to be a valuable tool in which parameter settings can be varied a posterori and the optimal setting can be determined for each measurement. Moreover, the

  11. Investigation of Increased Mercury Levels in the Fisheries of Lower East Fork Poplar Creek (LEFPC), Oak Ridge Reservation, Tennessee

    International Nuclear Information System (INIS)

    Byrne-Kelly, D.; Cornish, J.; Hart, A.; Southworth, G.; Simms, L.

    2006-01-01

    The DOE Western Environmental Technology Office (WETO) is supporting Oak Ridge's remediation efforts by performing this study. MSE Technology Applications, Inc. (MSE) has performed a series of literature reviews and bench-scale testing to further evaluate the mercury problem in the Lower East Fork Poplar Creek (LEFPC) at Oak Ridge. The primary problem is that total mercury (HgT) levels in LEFPC water decrease, while HgT levels in sunfish muscle tissue increase, with distance away from the National Security Complex (NSC), despite extensive source control efforts at the facility. Furthermore, dissolved methylmercury (d-MeHg) levels increase downstream from the NSC, especially during warm weather and/or high flow events. MSE performed four test series that focused on conversion of dissolved and colloidal forms of elemental mercury (Hg deg.A) to methyl mercury (MeHg) by algal-bacterial bio-films (periphyton) present in the stream-bed of LEFPC; MeHg production by these bio-films under anoxic versus oxic conditions was the critical measurement taken. The bench-scale testing for Phase I was completed November 2005. The final reporting and the planning for Phase II testing are in progress. (authors)

  12. Organic compounds in Elm Fork Trinity River water used for public supply near Carrollton, Texas, 2002-05

    Science.gov (United States)

    Ging, Patricia B.; Delzer, Gregory C.; Hamilton, Pixie A.

    2009-01-01

    Organic compounds studied in this U.S. Geological Survey (USGS) assessment generally are man-made, including pesticides, solvents, gasoline hydrocarbons, personal-care and domestic-use products, refrigerants, and propellants. A total of 103 of 277 compounds were detected at least once among the 30 samples of source water for a community water system on the Elm Fork Trinity River near Carrollton, Texas, collected approximately monthly during 2002-05. The diversity of compounds detected indicates a variety of different sources and uses (including wastewater discharge, industrial, agricultural, domestic, and others) and different pathways (including overland runoff and groundwater discharge) to drinking-water supplies. Nine compounds were detected year-round in source-water samples, including chloroform, methyl tert-butyl ether (MTBE), and selected herbicide compounds commonly used in the Trinity River Basin and in other urban areas across the United States. About 90 percent of the 42 compounds detected most frequently in source water (in at least 20 percent of the samples) also were detected most frequently in finished water (after treatment but before distribution). Concentrations for all detected compounds in source and finished water generally were less than 0.1 microgram per liter and always less than human-health benchmarks, which are available for about one-half of the detected compounds.

  13. Postremediation monitoring program baseline assessment report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Greeley, M.S. Jr.; Ashwood, T.L.; Kszos, L.A.; Peterson, M.J.; Rash, C.D.; Southworth, G.R. [Oak Ridge National Lab., TN (United States); Phipps, T.L. [CKY, Inc. (United States)

    1998-04-01

    Lower East Fork Poplar Creek (LEFPC) and its floodplain are contaminated with mercury (Hg) from ongoing and historical releases from the US Department of Energy (DOE) Oak Ridge Y-12 Plant. A remedial investigation and feasibility study of LEFPC resulted in the signing of a Record of Decision (ROD) in August 1995. In response to the ROD, soil contaminated with mercury above 400 mg/kg was removed from two sites in LEFPC and the floodplain during a recently completed remedial action (RA). The Postremediation Monitoring Program (PMP) outlined in the LEFPC Monitoring Plan was envisioned to occur in two phases: (1) a baseline assessment prior to remediation and (2) postremediation monitoring. The current report summarizes the results of the baseline assessment of soil, water, biota, and groundwater usage in LEFPC and its floodplain conducted in 1995 and 1996 by personnel of the Oak Ridge National Laboratory Biological Monitoring and Abatement Program (BMAP). This report also includes some 1997 data from contaminated sites that did not undergo remediation during the RA (i.e., sites where mercury is greater than 200 mg/kg but less than 400 mg/kg). The baseline assessment described in this document is distinct and separate from both the remedial investigation/feasibility study the confirmatory sampling conducted by SAIC during the RA. The purpose of the current assessment was to provide preremediation baseline data for the LEFPC PMP outlined in the LEFPC Monitoring Plan, using common approaches and techniques, as specified in that plan.

  14. A quartz enhanced photo-acoustic gas sensor based on a custom tuning fork and a terahertz quantum cascade laser.

    Science.gov (United States)

    Patimisco, Pietro; Borri, Simone; Sampaolo, Angelo; Beere, Harvey E; Ritchie, David A; Vitiello, Miriam S; Scamarcio, Gaetano; Spagnolo, Vincenzo

    2014-05-07

    An innovative quartz enhanced photoacoustic (QEPAS) gas sensing system operating in the THz spectral range and employing a custom quartz tuning fork (QTF) is described. The QTF dimensions are 3.3 cm × 0.4 cm × 0.8 cm, with the two prongs spaced by ∼800 μm. To test our sensor we used a quantum cascade laser as the light source and selected a methanol rotational absorption line at 131.054 cm(-1) (∼3.93 THz), with line-strength S = 4.28 × 10(-21) cm mol(-1). The sensor was operated at 10 Torr pressure on the first flexion QTF resonance frequency of 4245 Hz. The corresponding Q-factor was 74 760. Stepwise concentration measurements were performed to verify the linearity of the QEPAS signal as a function of the methanol concentration. The achieved sensitivity of the system is 7 parts per million in 4 seconds, corresponding to a QEPAS normalized noise-equivalent absorption of 2 × 10(-10) W cm(-1) Hz(-1/2), comparable with the best result of mid-IR QEPAS systems.

  15. Second report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Hinzman, R.L. [ed.; Adams, S.M. [Oak Ridge National Lab., TN (United States); Black, M.C. [Oklahoma State Univ., Stillwater, OK (United States)] [and others

    1993-06-01

    As stipulated in the National Pollutant Discharge Elimination System (NDPES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of BMAP are (1) to demonstrate that the current effluent limitations established for the Y-12 Plant protect the classified uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) and (2) to document the ecological effects resulting from implementation of a Water Pollution Control Program that includes construction of several large wastewater treatment facilities. BMAP consists of four major tasks: (1) ambient toxicity testing; (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic (bottom-dwelling) macroinvertebrates, and fish. This document, the second in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted between July 1986 and July 1988, although additional data collected outside this time period are included, as appropriate.

  16. Wear Analysis of Die Inserts in the Hot Forging Process of a Forked Type Forging Using Reverse Scanning Techniques

    Directory of Open Access Journals (Sweden)

    Łukasz Dworzak

    2017-12-01

    Full Text Available This article presents a wear analysis of die inserts used in the hot forging process of a forked forging (yoke, an element applied in steering systems of passenger vehicles. Studies involved the application of an original reverse scanning method intended for rapid and reliable wear analysis of forging tools (with complicated shape affording easy assessment without the need to dismount tools from the forging unit. The developed method involves analysis of progressive wear of forging tools based on measurements (scanning of forgings periodically collected from the process and constitutes a useful tool for measurement and testing. As the authors’ earlier works have demonstrated, the proposed new approach to analysis of tool wear with the application of reverse 3D scanning has proven successful in multiple instances in the case of axially symmetrical objects. The presented results of studies indicate that it is possible to utilize the expanded method to analyze the lifetime of forging tools, including tools with complex geometry. Application of the reverse scanning method allows for continuous and practical monitoring of the condition of forging tools over the course of the forging process and should have a positive impact on improving production output and reducing production costs.

  17. Second report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    International Nuclear Information System (INIS)

    Hinzman, R.L.; Black, M.C.

    1993-06-01

    As stipulated in the National Pollutant Discharge Elimination System (NDPES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1986, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of BMAP are (1) to demonstrate that the current effluent limitations established for the Y-12 Plant protect the classified uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) and (2) to document the ecological effects resulting from implementation of a Water Pollution Control Program that includes construction of several large wastewater treatment facilities. BMAP consists of four major tasks: (1) ambient toxicity testing; (2) bioaccumulation studies; (3) biological indicator studies; and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic (bottom-dwelling) macroinvertebrates, and fish. This document, the second in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted between July 1986 and July 1988, although additional data collected outside this time period are included, as appropriate

  18. Procedure for calculating estimated ultimate recoveries of Bakken and Three Forks Formations horizontal wells in the Williston Basin

    Science.gov (United States)

    Cook, Troy A.

    2013-01-01

    Estimated ultimate recoveries (EURs) are a key component in determining productivity of wells in continuous-type oil and gas reservoirs. EURs form the foundation of a well-performance-based assessment methodology initially developed by the U.S. Geological Survey (USGS; Schmoker, 1999). This methodology was formally reviewed by the American Association of Petroleum Geologists Committee on Resource Evaluation (Curtis and others, 2001). The EUR estimation methodology described in this paper was used in the 2013 USGS assessment of continuous oil resources in the Bakken and Three Forks Formations and incorporates uncertainties that would not normally be included in a basic decline-curve calculation. These uncertainties relate to (1) the mean time before failure of the entire well-production system (excluding economics), (2) the uncertainty of when (and if) a stable hyperbolic-decline profile is revealed in the production data, (3) the particular formation involved, (4) relations between initial production rates and a stable hyperbolic-decline profile, and (5) the final behavior of the decline extrapolation as production becomes more dependent on matrix storage.

  19. The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast.

    Science.gov (United States)

    Larsen, Nicolai B; Sass, Ehud; Suski, Catherine; Mankouri, Hocine W; Hickson, Ian D

    2014-04-07

    Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.

  20. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant

    International Nuclear Information System (INIS)

    1992-02-01

    This report contains groundwater quality data obtained during the 1991 calendar year at several waste management facilities and petroleum fuel underground storage tank (UST) sites associated with the Y-12 Plant. These sites are within the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three regimes defined for the purposes of groundwater and surface-water quality monitoring and remediation. This report was prepared for informational purposes. Included are the analytical data for groundwater samples collected from selected monitoring wells during 1991 and the results for quality assurance/quality control (QA/QC) samples associated with each groundwater sample. This report also contains summaries of selected data, including ion-charge balances for each groundwater sample, a summary of analytical results for nitrate (a principle contaminant in the UEFPCHR), results of volatile organic compounds (VOCs) analyses validated using the associated QA/QC sample data, a summary of trace metal concentrations which exceeded drinking-water standards, and a summary of radiochemical analyses and associated counting errors

  1. Postremediation monitoring program baseline assessment report, Lower East Fork Poplar Creek, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Greeley, M.S. Jr.; Ashwood, T.L.; Kszos, L.A.; Peterson, M.J.; Rash, C.D.; Southworth, G.R.; Phipps, T.L.

    1998-04-01

    Lower East Fork Poplar Creek (LEFPC) and its floodplain are contaminated with mercury (Hg) from ongoing and historical releases from the US Department of Energy (DOE) Oak Ridge Y-12 Plant. A remedial investigation and feasibility study of LEFPC resulted in the signing of a Record of Decision (ROD) in August 1995. In response to the ROD, soil contaminated with mercury above 400 mg/kg was removed from two sites in LEFPC and the floodplain during a recently completed remedial action (RA). The Postremediation Monitoring Program (PMP) outlined in the LEFPC Monitoring Plan was envisioned to occur in two phases: (1) a baseline assessment prior to remediation and (2) postremediation monitoring. The current report summarizes the results of the baseline assessment of soil, water, biota, and groundwater usage in LEFPC and its floodplain conducted in 1995 and 1996 by personnel of the Oak Ridge National Laboratory Biological Monitoring and Abatement Program (BMAP). This report also includes some 1997 data from contaminated sites that did not undergo remediation during the RA (i.e., sites where mercury is greater than 200 mg/kg but less than 400 mg/kg). The baseline assessment described in this document is distinct and separate from both the remedial investigation/feasibility study the confirmatory sampling conducted by SAIC during the RA. The purpose of the current assessment was to provide preremediation baseline data for the LEFPC PMP outlined in the LEFPC Monitoring Plan, using common approaches and techniques, as specified in that plan

  2. Comparison of quartz tuning forks and AlN-based extensional microresonators for viscosity measurements in oil/fuel mixtures

    Science.gov (United States)

    Toledo, J.; Manzaneque, T.; Hernando-García, J.; Vazquez, J.; Ababneh, A.; Seidel, H.; Lapuerta, M.; Sánchez-Rojas, J. L.

    2013-05-01

    In-situ monitoring of the physical properties of liquids is of great interest in the automotive industry. For example, lubricants are subject to dilution with diesel fuel as a consequence of late-injection processes, which are necessary for regenerating diesel particulate filters. This dilution can be determined by tracking the viscosity and the density of the lubricant. Here we report the test of two in-plane movement based resonators to explore their capability to monitor oil dilution with diesel and biodiesel. One of the resonators is the commercially available millimeter-sized quartz tuning fork, working at 32.7 kHz. The second resonator is a state-of-the-art micron-sized AlN-based rectangular plate, actuated in the first extensional mode in the MHz range. Electrical impedance measurements were carried out to characterize the performance of the structures in various liquid media in a wide range of viscosities. These measurements were completed with the development of low-cost electronic circuits to track the resonance frequency and the quality factor automatically, these two parameters allow to obtain the viscosity of various fluids under investigation, as in the case of dilution of lubricant SAE 15W40 and biodiesel.

  3. Measurements on spent-fuel assemblies at Arkansas Nuclear One using the Fork system. Final report, January 1995

    International Nuclear Information System (INIS)

    Ewing, R.I.; Bronowski, D.R.; Bosler, G.E.; Siebelist, R.; Priore, J.; Hansford, C.H.; Sullivan, S.

    1997-03-01

    The Fork measurement system has been used to examine spent-fuel assemblies at the two reactors of Arkansas Nuclear One, operated by Entergy Operations, Inc. The Unit 1 reactor is a Babcock and Wilcox (B and W) design, and the Unit 2 reactor is a Combustion Engineering (CE) design. The neutron and gamma-ray emissions from individual spent-fuel assemblies were measured in the storage pools by raising each assembly pathway out of the storage rack and performing a measurement near the center of the assembly. The overall accuracy of the measurements after corrections is about 2%. Thirty-four assemblies were examined at Unit 1, and forty-one assemblies at Unit 2. The average deviation of the burnup measurements from the calibration was 3.0% at Unit 1 and 3.5% at Unit 2, indicating 2 to 3% random variation among the reactor records. There was no indication of clearly anomalous assemblies. Axial Scans of the variation in neutron and gamma ray emission were obtained by collecting data at several locations along the length of three assemblies at Unit 2. Two of these assemblies were nonstandard in that each contained a small neutron source. The sources were detected by the axial scans. The test program was a cooperative effort involving Sandia National Laboratories, Los Alamos National Laboratory, Entergy Operations, Inc., the Electric Power Research Institute, and the Office of Civilian Radioactive Waste Management of the US Department of Energy

  4. First report on the Oak Ridge Y-12 Plant Biological Monitoring and Abatement Program for East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Loar, J.M.; Adams, S.M.; Allison, L.J.; Boston, H.L.; Huston, M.A.; McCarthy, J.F.; Smith, J.G.; Southworth, G.R.; Stewart, A.J. (Oak Ridge National Lab., TN (United States)); Black, M.C. (Oklahoma State Univ., Stillwater, OK (United States)); Gatz, A.J. Jr. (Ohio Wesleyan Univ., Delaware, OH (United States)); Hinzman, R.L. (Oak Ridge Research Inst., TN (United States)); Jimenez, B.D. (Puerto Rico Univ.,

    1992-07-01

    As stipulated in the National Pollutant Discharge Elimination System (NPDES) permit issued to the Oak Ridge Y-12 Plant on May 24, 1985, a Biological Monitoring and Abatement Program (BMAP) was developed for the receiving stream, East Fork Poplar Creek (EFPC). The objectives of the BMAP are (1) to demonstrate that the current effluent limitations established for the Oak Ridge Y-12 Plant protect the uses of EFPC (e.g., the growth and propagation of fish and aquatic life), as designated by the Tennessee Department of Environment and Conservation (TDEC) [formerly the Tennessee Department of Health and Environment (TDHE)], and (2) to document the ecological effects resulting from implementation of a water pollution control program that includes construction of several large wastewater treatment facilities. The BMAP consists of four major tasks: (1) ambient toxicity testing, (2) bioaccumulation studies, (3) biological indicator studies, and (4) ecological surveys of stream communities, including periphyton (attached algae), benthic macroinvertebrates, and fish. This document, the first in a series of reports on the results of the Y-12 Plant BMAP, describes studies that were conducted from May 1985 through September 1986.

  5. Design and Vibration Sensitivity Analysis of a MEMS Tuning Fork Gyroscope with an Anchored Diamond Coupling Mechanism

    Directory of Open Access Journals (Sweden)

    Yanwei Guan

    2016-04-01

    Full Text Available In this paper, a new micromachined tuning fork gyroscope (TFG with an anchored diamond coupling mechanism is proposed while the mode ordering and the vibration sensitivity are also investigated. The sense-mode of the proposed TFG was optimized through use of an anchored diamond coupling spring, which enables the in-phase mode frequency to be 108.3% higher than the anti-phase one. The frequencies of the in- and anti-phase modes in the sense direction are 9799.6 Hz and 4705.3 Hz, respectively. The analytical solutions illustrate that the stiffness difference ratio of the in- and anti-phase modes is inversely proportional to the output induced by the vibration from the sense direction. Additionally, FEM simulations demonstrate that the stiffness difference ratio of the anchored diamond coupling TFG is 16.08 times larger than the direct coupling one while the vibration output is reduced by 94.1%. Consequently, the proposed new anchored diamond coupling TFG can structurally increase the stiffness difference ratio to improve the mode ordering and considerably reduce the vibration sensitivity without sacrificing the scale factor.

  6. Environmental Assessment Addressing Privatization of Military Family Housing at Grand Forks Air Force Base, North Dakota

    Science.gov (United States)

    2011-05-01

    falls within a range of 10 to 25 dBA for normal hearing. The threshold of pain occurs at the upper boundary of audibility, which is normally in the...aquifer is found in the Ordovician-aged Red River Formation. Yield varies depending on joints and fractures within the formation, and the...oc ia tio n an d th e N at io na l T rib al E nv iro nm en ta l C ou nc il. T he e m is si on fa ct or is a ss um ed to e nc om pa ss a

  7. Evaluation of Calendar Year 1996 groundwater and surface water quality data for the Upper East Fork Poplar Creek Hydrogeologic Regime at the US Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1997-09-01

    This report presents an evaluation of the groundwater monitoring data obtained in the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) during calendar year (CY) 1996. The East Fork Regime encompasses several confirmed and suspected sources of groundwater contamination within industrialized areas of the US Department of Energy (DOE) Y-12 Plant in Bear Creek Valley (BCV) southeast of Oak Ridge, Tennessee. The CY 1996 groundwater and surface water monitoring data are presented in Calendar Year 1996 Annual Groundwater Monitoring Report for the Upper East Fork Poplar Creek Hydrogeologic Regime at the US Department of Energy Y-12 Plant, Oak Ridge, Tennessee, along with the required data evaluations specified in the Resource Conservation and Recovery Act (RCRA) post-closure permit for the East Fork Regime. This report provides additional evaluation of the CY 1996 groundwater and surface water monitoring data with an emphasis on regime-wide groundwater contamination and long-term concentration trends for regulated and non-regulated monitoring parameters

  8. Development of CE-QUAL-W2 models for the Middle Fork Willamette and South Santiam Rivers, Oregon

    Science.gov (United States)

    Buccola, Norman L.; Stonewall, Adam J.; Sullivan, Annett B.; Kim, Yoonhee; Rounds, Stewart A.

    2013-01-01

    Hydrodynamic (CE-QUAL-W2) models of Hills Creek Lake (HCL), Lookout Point Lake (LOP), and Dexter Lake (DEX) on the Middle Fork Willamette River (MFWR), and models of Green Peter Lake and Foster Lake on the South Santiam River systems in western Oregon were updated and recalibrated for a wide range of flow and meteorological conditions. These CE-QUAL-W2 models originally were developed by West Consultants, Inc., for the U.S. Army Corps of Engineers. This study by the U.S. Geological Survey included a reassessment of the models’ calibration in more recent years—2002, 2006, 2008, and 2011—categorized respectively as low, normal, high, and extremely high flow calendar years. These years incorporated current dam-operation practices and more available data than the time period used in the original calibration. Modeled water temperatures downstream of both HCL and LOP-DEX on the MFWR were within an average of 0.68 degree Celsius (°C) of measured values; modeled temperatures downstream of Foster Dam on the South Santiam River were within an average of 0.65°C of measured values. A new CE-QUAL-W2 model was developed and calibrated for the riverine MFWR reach between Hills Creek Dam and the head of LOP, allowing an evaluation of the flow and temperature conditions in the entire MFWR system from HCL to Dexter Dam. The complex bathymetry and long residence time of HCL, combined with the relatively deep location of the power and regulating outlet structures at Hills Creek Dam, led to a HCL model that was highly sensitive to several outlet and geometric parameters related to dam structures (STR TOP, STR BOT, STR WIDTH). Release temperatures from HCL were important and often persisted downstream as they were incorporated in the MFWR model and the LOP-DEX model (downstream of MFWR). The models tended to underpredict the measured temperature of water releases from Dexter Dam during the late-September-through-December drawdown period in 2002, and again (to a lesser extent) in

  9. Assessment of conservation easements, total phosphorus, and total suspended solids in West Fork Beaver Creek, Minnesota, 1999-2012

    Science.gov (United States)

    Christensen, Victoria G.; Kieta, Kristen A.

    2014-01-01

    This study examined conservation easements and their effectiveness at reducing phosphorus and solids transport to streams. The U.S. Geological Survey cooperated with the Minnesota Board of Water and Soil Resources and worked collaboratively with the Hawk Creek Watershed Project to examine the West Fork Beaver Creek Basin in Renville County, which has the largest number of Reinvest In Minnesota land retirement contracts in the State (as of 2013). Among all conservation easement programs, a total of 24,218 acres of agricultural land were retired throughout Renville County, and 2,718 acres were retired in the West Fork Beaver Creek Basin from 1987 through 2012. Total land retirement increased steadily from 1987 until 2000. In 2000, land retirement increased sharply because of the Minnesota River Conservation Reserve Enhancement Program, then leveled off when the program ended in 2002. Streamflow data were collected during 1999 through 2011, and total phosphorus and total suspended solids data were collected during 1999 through 2012. During this period, the highest peak streamflow of 1,320 cubic feet per second was in March 2010. Total phosphorus and total suspended solids are constituents that tend to increase with increases in streamflow. Annual flow-weighted mean total-phosphorus concentrations ranged from 0.140 to 0.759 milligrams per liter, and annual flow-weighted mean total suspended solids concentrations ranged from 21.3 to 217 milligrams per liter. Annual flow-weighted mean total phosphorus and total suspended solids concentrations decreased steadily during the first 4 years of water-quality sample collection. A downward trend in flow-weighted mean total-phosphorus concentrations was significant from 1999 through 2008; however, flow-weighted total-phosphorus concentrations increased substantially in 2009, and the total phosphorus trend was no longer significant. The high annual flow-weighted mean concentrations for total phosphorus and total suspended solids

  10. The Drosophila FoxA ortholog Fork head regulates growth and gene expression downstream of Target of rapamycin.

    Directory of Open Access Journals (Sweden)

    Margret H Bülow

    2010-12-01

    Full Text Available Forkhead transcription factors of the FoxO subfamily regulate gene expression programs downstream of the insulin signaling network. It is less clear which proteins mediate transcriptional control exerted by Target of rapamycin (TOR signaling, but recent studies in nematodes suggest a role for FoxA transcription factors downstream of TOR. In this study we present evidence that outlines a similar connection in Drosophila, in which the FoxA protein Fork head (FKH regulates cellular and organismal size downstream of TOR. We find that ectopic expression and targeted knockdown of FKH in larval tissues elicits different size phenotypes depending on nutrient state and TOR signaling levels. FKH overexpression has a negative effect on growth under fed conditions, and this phenotype is not further exacerbated by inhibition of TOR via rapamycin feeding. Under conditions of starvation or low TOR signaling levels, knockdown of FKH attenuates the size reduction associated with these conditions. Subcellular localization of endogenous FKH protein is shifted from predominantly cytoplasmic on a high-protein diet to a pronounced nuclear accumulation in animals with reduced levels of TOR or fed with rapamycin. Two putative FKH target genes, CG6770 and cabut, are transcriptionally induced by rapamycin or FKH expression, and silenced by FKH knockdown. Induction of both target genes in heterozygous TOR mutant animals is suppressed by mutations in fkh. Furthermore, TOR signaling levels and FKH impact on transcription of the dFOXO target gene d4E-BP, implying a point of crosstalk with the insulin pathway. In summary, our observations show that an alteration of FKH levels has an effect on cellular and organismal size, and that FKH function is required for the growth inhibition and target gene induction caused by low TOR signaling levels.

  11. The Western progression of lyme disease: infectious and Nonclonal Borrelia burgdorferi Sensu Lato populations in Grand Forks County, North Dakota.

    Science.gov (United States)

    Stone, Brandee L; Russart, Nathan M; Gaultney, Robert A; Floden, Angela M; Vaughan, Jefferson A; Brissette, Catherine A

    2015-01-01

    Scant attention has been paid to Lyme disease, Borrelia burgdorferi, Ixodes scapularis, or reservoirs in eastern North Dakota despite the fact that it borders high-risk counties in Minnesota. Recent reports of B. burgdorferi and I. scapularis in North Dakota, however, prompted a more detailed examination. Spirochetes cultured from the hearts of five rodents trapped in Grand Forks County, ND, were identified as B. burgdorferi sensu lato through sequence analyses of the 16S rRNA gene, the 16S rRNA gene-ileT intergenic spacer region, flaB, ospA, ospC, and p66. OspC typing revealed the presence of groups A, B, E, F, L, and I. Two rodents were concurrently carrying multiple OspC types. Multilocus sequence typing suggested the eastern North Dakota strains are most closely related to those found in neighboring regions of the upper Midwest and Canada. BALB/c mice were infected with B. burgdorferi isolate M3 (OspC group B) by needle inoculation or tick bite. Tibiotarsal joints and ear pinnae were culture positive, and B. burgdorferi M3 was detected by quantitative PCR (qPCR) in the tibiotarsal joints, hearts, and ear pinnae of infected mice. Uninfected larval I. scapularis ticks were able to acquire B. burgdorferi M3 from infected mice; M3 was maintained in I. scapularis during the molt from larva to nymph; and further, M3 was transmitted from infected I. scapularis nymphs to naive mice, as evidenced by cultures and qPCR analyses. These results demonstrate that isolate M3 is capable of disseminated infection by both artificial and natural routes of infection. This study confirms the presence of unique (nonclonal) and infectious B. burgdorferi populations in eastern North Dakota. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  12. Farm to Fork Quantitative Risk Assessment of Listeria monocytogenes Contamination in Raw and Pasteurized Milk Cheese in Ireland.

    Science.gov (United States)

    Tiwari, Uma; Cummins, Enda; Valero, Antonio; Walsh, Des; Dalmasso, Marion; Jordan, Kieran; Duffy, Geraldine

    2015-06-01

    The objective of this study was to model and quantify the level of Listeria monocytogenes in raw milk cheese (RMc) and pasteurized milk cheese (PMc) from farm to fork using a Bayesian inference approach combined with a quantitative risk assessment. The modeling approach included a prediction of contamination arising from the farm environment as well from cross-contamination within the cheese-processing facility through storage and subsequent human exposure. The model predicted a high concentration of L. monocytogenes in contaminated RMc (mean 2.19 log10 CFU/g) compared to PMc (mean -1.73 log10 CFU/g). The mean probability of illness (P1 for low-risk population, LR) and (P2 for high-risk population, HR, e.g., immunocompromised) adult Irish consumers following exposure to contaminated cheese was 7 × 10(-8) (P1 ) and 9 × 10(-4) (P2 ) for RMc and 7 × 10(-10) (P1 ) and 8 × 10(-6) (P2 ) for PMc, respectively. In addition, the model was used to evaluate performance objectives at various stages, namely, the cheese making and ripening stages, and to set a food safety objective at the time of consumption. A scenario analysis predicted various probabilities of L. monocytogenes contamination along the cheese-processing chain for both RMc and PMc. The sensitivity analysis showed the critical factors for both cheeses were the serving size of the cheese, storage time, and temperature at the distribution stage. The developed model will allow food processors and policymakers to identify the possible routes of contamination along the cheese-processing chain and to reduce the risk posed to human health. © 2015 Society for Risk Analysis.

  13. Hg isotopes reveal in-stream processing and legacy inputs in East Fork Poplar Creek, Oak Ridge, Tennessee, USA.

    Science.gov (United States)

    Demers, Jason D; Blum, Joel D; Brooks, Scott C; Donovan, Patrick M; Riscassi, Ami L; Miller, Carrie L; Zheng, Wang; Gu, Baohua

    2018-04-25

    Natural abundance stable Hg isotope measurements were used to place new constraints on sources, transport, and transformations of Hg along the flow path of East Fork Poplar Creek (EFPC), a point-source contaminated headwater stream in Oak Ridge, Tennessee. Particulate-bound Hg in the water column of EFPC within the Y-12 National Security Complex, was isotopically similar to average metallic Hg(0) used in industry, having a mean δ202Hg value of -0.42 ± 0.09‰ (1SD) and near-zero Δ199Hg. On average, particulate fraction δ202Hg values increased downstream by 0.53‰, while Δ199Hg decreased by -0.10‰, converging with the Hg isotopic composition of the fine fraction of streambed sediment along the 26 km flow path. The dissolved fraction behaved differently. Although initial Δ199Hg values of the dissolved fraction were also near-zero, these values increased transiently along the flow path. Initial δ202Hg values of the dissolved fraction were more variable than in the particulate fraction, ranging from -0.44 to 0.18‰ among three seasonal sampling campaigns, but converged to an average δ202Hg value of 0.01 ± 0.10‰ (1SD) downstream. Dissolved Hg in the hyporheic and riparian pore water had higher and lower δ202Hg values, respectively, compared to dissolved Hg in stream water. Variations in Hg isotopic composition of the dissolved and suspended fractions along the flow path suggest that: (1) physical processes such as dilution and sedimentation do not fully explain decreases in total mercury concentrations along the flow path; (2) in-stream processes include photochemical reduction, but microbial reduction is likely more dominant; and (3) additional sources of dissolved mercury inputs to EFPC at baseflow during this study predominantly arise from the hyporheic zone.

  14. Second report on the Oak Ridge Y-12 Plant fish kill for Upper East Fork Poplar Creek

    International Nuclear Information System (INIS)

    Etnier, E.L.; Opresko, D.M.; Talmage, S.S.

    1994-08-01

    This report summarizes the monitoring of fish kills in upper East Fork Poplar Creek (EFPC) from July 1990 to June 1993. Since the opening of Lake Reality (LR) in 1988, total numbers of fish inhabiting upper EFPC have increased. However, species diversity has remained poor. Water quality data have been collected in upper EFPC during the time period covered in this report. Total residual chlorine (TRC) levels have exceeded federal and state water quality criteria over the years. However, with the installation of two dechlorination systems in late 1992, TRC levels have been substantially lowered in most portions of upper EFPC. By June 1993, concentrations of TRC were 0.04 to 0.06 mg/L at the north-south pipes (NSP) and below detection limits at sampling station AS-8 and were 0 to 0.01 mg/L at the inlet and outlet of LR. The daily chronic fish mortality in upper EFPC has been attributed to background stress resulting from the continuous discharge of chlorine into upper EFPC. Mean daily mortality rates for 22 acute fish kills were three fold or more above background and usually exceeded ten fish per day. Total number of dead fish collected per acute kill event ranged from 30 to over 1,000 fish; predominant species killed were central stonerollers (Campostoma anomalum) and striped shiners (Luxilus chrysocephalus). Spills or elevated releases of toxic chemicals, such as acids, organophosphates, aluminum nitrate, ammonia, or chlorine, were identified as possible causative agents; however, a definitive cause-effect relationship was rarely established for any acute kills. Ambient toxicity testing, in situ chemical monitoring, and streamside experiments were used to examine TRC dynamics and ambient toxicity in EFPC

  15. Second report on the Oak Ridge Y-12 Plant fish kill for Upper East Fork Poplar Creek

    Energy Technology Data Exchange (ETDEWEB)

    Etnier, E.L.; Opresko, D.M.; Talmage, S.S. [eds.

    1994-08-01

    This report summarizes the monitoring of fish kills in upper East Fork Poplar Creek (EFPC) from July 1990 to June 1993. Since the opening of Lake Reality (LR) in 1988, total numbers of fish inhabiting upper EFPC have increased. However, species diversity has remained poor. Water quality data have been collected in upper EFPC during the time period covered in this report. Total residual chlorine (TRC) levels have exceeded federal and state water quality criteria over the years. However, with the installation of two dechlorination systems in late 1992, TRC levels have been substantially lowered in most portions of upper EFPC. By June 1993, concentrations of TRC were 0.04 to 0.06 mg/L at the north-south pipes (NSP) and below detection limits at sampling station AS-8 and were 0 to 0.01 mg/L at the inlet and outlet of LR. The daily chronic fish mortality in upper EFPC has been attributed to background stress resulting from the continuous discharge of chlorine into upper EFPC. Mean daily mortality rates for 22 acute fish kills were three fold or more above background and usually exceeded ten fish per day. Total number of dead fish collected per acute kill event ranged from 30 to over 1,000 fish; predominant species killed were central stonerollers (Campostoma anomalum) and striped shiners (Luxilus chrysocephalus). Spills or elevated releases of toxic chemicals, such as acids, organophosphates, aluminum nitrate, ammonia, or chlorine, were identified as possible causative agents; however, a definitive cause-effect relationship was rarely established for any acute kills. Ambient toxicity testing, in situ chemical monitoring, and streamside experiments were used to examine TRC dynamics and ambient toxicity in EFPC.

  16. High quality-factor quartz tuning fork glass probe used in tapping mode atomic force microscopy for surface profile measurement

    Science.gov (United States)

    Chen, Yuan-Liu; Xu, Yanhao; Shimizu, Yuki; Matsukuma, Hiraku; Gao, Wei

    2018-06-01

    This paper presents a high quality-factor (Q-factor) quartz tuning fork (QTF) with a glass probe attached, used in frequency modulation tapping mode atomic force microscopy (AFM) for the surface profile metrology of micro and nanostructures. Unlike conventionally used QTFs, which have tungsten or platinum probes for tapping mode AFM, and suffer from a low Q-factor influenced by the relatively large mass of the probe, the glass probe, which has a lower density, increases the Q-factor of the QTF probe unit allowing it to obtain better measurement sensitivity. In addition, the process of attaching the probe to the QTF with epoxy resin, which is necessary for tapping mode AFM, is also optimized to further improve the Q-factor of the QTF glass probe. The Q-factor of the optimized QTF glass probe unit is demonstrated to be very close to that of a bare QTF without a probe attached. To verify the effectiveness and the advantages of the optimized QTF glass probe unit, the probe unit is integrated into a home-built tapping mode AFM for conducting surface profile measurements of micro and nanostructures. A blazed grating with fine tool marks of 100 nm, a microprism sheet with a vertical amplitude of 25 µm and a Fresnel lens with a steep slope of 90 degrees are used as measurement specimens. From the measurement results, it is demonstrated that the optimized QTF glass probe unit can achieve higher sensitivity as well as better stability than conventional probes in the measurement of micro and nanostructures.

  17. Geomorphic response of the North Fork Stillaguamish River to the State Route 530 landslide near Oso, Washington

    Science.gov (United States)

    Anderson, Scott W.; Keith, Mackenzie K.; Magirl, Christopher S.; Wallick, J. Rose; Mastin, Mark C.; Foreman, James R.

    2017-08-03

    On March 22, 2014, the State Route 530 Landslide near Oso, Washington mobilized 8 million cubic meters of unconsolidated Pleistocene material, creating a valley‑spanning deposit that fully impounded the North Fork Stillaguamish River. The river overtopped the 8-meter high debris impoundment within 25 hours and began steadily incising a new channel through the center of the deposit. Repeat topographic surveys, sediment transport measurements, bedload transport models, and observations of downstream channel change were used to document the establishment of that new channel through the landslide and assess the potential for downstream aggradation or channel change that might increase downstream flood hazards.Efficient erosion of the landslide deposit, associated with the steep knickzone formed by the downstream edge of the deposit, resulted in the re-establishment of a 20–40 meters wide, deeply inset channel through the entire deposit by May 2014, 2 months after the landslide. The mean water-surface elevation of the channel through the landslide decreased 7 meters during that 2-month period, and was about 1 meter above the pre-landslide profile in July 2014. The 2014–15 flood season, which included flows near the 0.5 annual exceedance probability discharge (2-year flood), widened the channel tens of meters, and further lowered the water-surface profile 0.5 meter. The planform position evolved slowly as a result of 5–20-meter high banks predominantly composed of clay-rich, cohesive lacustrine material. Erosion of the landslide deposit delivered a total of 820 thousand metric tons of sediment to the North Fork Stillaguamish River over the 18 months following the landslide. The sediment delivery from the deposit was predominantly fine grained: 77 percent (by mass) of the eroded material was silt or clay (less than 0.063 millimeter [mm]), 19 percent sand (0.063–2 mm), and 4 percent pebbles and cobbles (greater than 2 mm).Over the 18 months following the

  18. Physical habitat classification and instream flow modeling to determine habitat availability during low-flow periods, North Fork Shenandoah River, Virginia

    Science.gov (United States)

    Krstolic, Jennifer L.; Hayes, Donald C.; Ruhl, Peter M.

    2006-01-01

    Increasing development and increasing water withdrawals for public, industrial, and agricultural water supply threaten to reduce streamflows in the Shenandoah River basin in Virginia. Water managers need more information to balance human water-supply needs with the daily streamflows necessary for maintaining the aquatic ecosystems. To meet the need for comprehensive information on hydrology, water supply, and instream-flow requirements of the Shenandoah River basin, the U.S. Geological Survey and the Northern Shenandoah Valley Regional Commission conducted a cooperative investigation of habitat availability during low-flow periods on the North Fork Shenandoah River. Historic streamflow data and empirical data on physical habitat, river hydraulics, fish community structure, and recreation were used to develop a physical habitat simulation model. Hydraulic measurements were made during low, medium, and high flows in six reaches at a total of 36 transects that included riffles, runs, and pools, and that had a variety of substrates and cover types. Habitat suitability criteria for fish were developed from detailed fish-community sampling and microhabitat observations. Fish were grouped into four guilds of species and life stages with similar habitat requirements. Simulated habitat was considered in the context of seasonal flow regimes to show the availability of flows that sustain suitable habitat during months when precipitation and streamflow are scarce. The North Fork Shenandoah River basin was divided into three management sections for analysis purposes: the upper section, middle section, and lower section. The months of July, August, and September were chosen to represent a low-flow period in the basin with low mean monthly flows, low precipitation, high temperatures, and high water withdrawals. Exceedance flows calculated from the combined data from these three months describe low-flow periods on the North Fork Shenandoah River. Long-term records from three

  19. Use of a watershed-modeling approach to assess hydrologic effects of urbanization, North Fork Pheasant Branch basin near Middleton, Wisconsin

    Science.gov (United States)

    Steuer, Jeffrey J.; Hunt, R.J.

    2001-01-01

    The North Fork Pheasant Branch Basin in Dane County, Wisconsin is expected to undergo development. There are concerns that development will adversely affect water resources with increased flood peaks, increased runoff volumes, and increased pollutant loads. To provide a scientific basis for evaluating the hydrologic system response to development the Precipitation Runoff Modeling System (PRMS) was used to model the upper Pheasant Branch Creek watershed with an emphasis on the North Fork Basin. The upper Pheasant Branch Creek (18.3 mi2; 11,700 acres) Basin was represented with 21 Hydrologic Response Units (daily time step) and 50 flow planes (5-minute time steps). Precipitation data from the basin outlet streamflow-gaging station located at Highway 12 and temperature data from a nearby airport were used to drive the model. Continuous discharge records at three gaging stations were used for model calibration. To qualitatively assess model representation of small subbasins, periodic reconnaissance, often including a depth measurement, was made after precipitation to determine the occurrence of flow in ditches and channels from small subbasins. As a further effort to verify the model on a small subbasin scale, continuous-stage sensors (15-minute intervals) measured depth at the outlets of three small subbasins (500 to 1,200 acres). Average annual precipitation for the simulation period from 1993 to 1998 was 35.2 inches. The model simulations showed that, on average, 23.9 inches were intercepted by vegetation, or lost to evapotranspiration, 6.0 inches were infiltrated and moved to the regional ground-water system, and 4.8 inches contributed to the upper Pheasant Branch streamflow. The largest runoff event during the calibration interval was in July 1993 (746 ft3/sec; with a recurrence interval of approximately 25 years). Resulting recharge rates from the calibrated model were subsequently used as input into a ground-water-flow model. Average annual recharge varied

  20. [Immunoprecipitation mapping of the TRX-associated chromosome elements in the fork head gene promoter in the Drosophila melanogaster salivary gland cells].

    Science.gov (United States)

    Riakhovskiĭ, A A; Tillib, S V

    2007-09-01

    Using the method of immunoprecipitation of the in vivo crosslinked and sheared by sonication chromatin, mapping of potential trithorax-associated regulatory elements within the extended (9 kb) promoter region of the fork head gene (fkh) in the Drosophila melanogaster salivary gland cells was performed. Relative homogeneity of the salivary gland cells, along with the parallel use of the antibodies to different domains of the same trithorax protein (TRX), and the introduction of cross-hybridization steps for additional specific enrichment of initial DNA libraries, provided improvement of the method effectiveness and identification of one major and two less expressed potential TRX-binding sites.

  1. Developing a Natural Gas-Powered Bus Rapid Transit Service: A Case Study on Leadership: Roaring Fork Transportation Authority (Presentation); NREL (National Renewable Energy Laboratory)

    Energy Technology Data Exchange (ETDEWEB)

    Schroeder, A.

    2015-03-01

    The Roaring Fork Transportation Authority (RFTA) represents a series of unique successes in alternative fuel deployment by pushing the envelope with innovative solutions. In the last year, RFTA demonstrated the ability to utilize compressed natural gas buses at a range of altitudes, across long distances, in extreme weather conditions and in a modern indoor fueling and maintenance facility - allwhile saving money and providing high-quality customer service. This case study will highlight how the leadership of organizations and communities that are implementing advances in natural gas vehicle technology is paving the way for broader participation.

  2. Assessment of water and proppant quantities associated with petroleum production from the Bakken and Three Forks Formations, Williston Basin Province, Montana and North Dakota, 2016

    Science.gov (United States)

    Haines, Seth; Varela, Brian A.; Hawkins, Sarah J.; Gianoutsos, Nicholas J.; Thamke, Joanna N.; Engle, Mark A.; Tennyson, Marilyn E.; Schenk, Christopher J.; Gaswirth, Stephanie B.; Marra, Kristen R.; Kinney, Scott A.; Mercier, Tracey J.; Martinez, Cericia D.

    2017-06-23

    The U.S. Geological Survey (USGS) has completed an assessment of water and proppant requirements and water production associated with the possible future production of undiscovered oil and gas resources in the Three Forks and Bakken Formations (Late Devonian to Early Mississippian) of the Williston Basin Province in Montana and North Dakota. This water and proppant assessment is directly linked to the geology-based assessment of the undiscovered, technically recoverable continuous oil and gas resources that is described in USGS Fact Sheet 2013–3013.

  3. Calendar year 1995 groundwater quality report for the upper east Fork Poplar Creek Hydrogeologic regime, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. 1995 Groundwater quality data and calculated rate of contaminant migration

    International Nuclear Information System (INIS)

    1996-02-01

    This annual groundwater quality report (GWQR) contains groundwater and surface water quality data obtained during the 1995 calendar year (CY) at several waste management facilities associated with the U.S. Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. The U.S. Environmental Protection Agency (EPA) identification number for the Y-12 Plant is TN3 89 009 0001. The sites addressed by this document are located within the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The East Fork Regime, which is one of three hydrogeologic regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant, encompasses the Y-12 Plant

  4. Calendar Year 1997 Annual Groundwater Monitoring Report For The Upper East Fork Poplar Creek Hydrogeologic Regime At The U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Jones, S.B.

    1998-02-01

    This report contains the groundwater monitoring data obtained during calendar year (CY) 1997 in compliance with the Resource Conservation Wd Recovery Act (RCRA) post-closure permit (PCP) for the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) at the U.S. Department of Energy (DOE) Y-12 Plant in Oak Ridge, Tennessee. Issued by the Tennessee Department of Environment and Conservation (TDEC), the PCP defines the RCRA post-closure corrective action monitoring requirements for the portion of the groundwater contaminant plume that has migrated into the East Fork Regime ftom the S-3 Ponds, a closed RCW-regulated former surface impoundment located in Bear Creek Valley near the west end of the Y-12 Plant. In addition to the RCIL4 post-closure corrective action monitoring results, this report contains the groundwater and surface water monitoring data obtained during CY 1997 to fulfill requirements of DOE Order 5400.1.

  5. Aging Brain, Aging Mind.

    Science.gov (United States)

    Selkoe, Dennis J.

    1992-01-01

    Discusses the aging process related to physical changes of the human neural structure involved in learning, memory, and reasoning. Presents evidence that indicates such alterations do not necessarily signal the decline in cognitive function. Vignettes provide images of brain structures involved in learning, memory, and reasoning; hippocampal…

  6. Current mercury distribution and bioavailability in floodplain soils of Lower East Fork Poplar Creek, Oak Ridge, Tennessee, USA

    International Nuclear Information System (INIS)

    Han, Fengxiang X.; Su, Yi; Monts, David L.

    2011-01-01

    The objectives of this study were to investigate the current status of mercury distribution, speciation and bioavailability in the floodplain soils of Lower East Fork Poplar Creek (LEFPC) after decades of US Department of Energy's remediation. Historically as part of its national security mission, the U.S. Department of Energy's Y-12 National Security Facility in Oak Ridge, TN, USA acquired a significant fraction of the world's supply of elemental mercury. During the 1950s and 1960s, a large amount of elemental mercury escaped confinement and is still present in the watershed surrounding the Y-12 facility. A series of remediation efforts have been deployed in the watersheds around the Oak Ridge site during the following years. The sampling fields were located in a floodplain of LEFPC of Oak Ridge, TN, USA. A series of surface soils (10-20 cm) were sampled from both wooded areas and wetland/grass land. Two 8x8 m fields were selected in the woodland. Five profiles each consisting of three layers were randomly taken from each field. The three layers were the surface layer at 0-10cm, subsurface layer at 50-60 cm, and bottom layer at 100-110 cm. Soil in both wood and wetland areas was well developed with a clear B horizon. The present study clearly shows that the total mercury in floodplain soils of LEFPC significantly decreased after the series of remediation. This study confirmed the long-term effectiveness of these remediation actions, especially after excavation of highly contaminated floodplain soils. However, the average total mercury level of all soil samples collected are in the range of 50-80 mg/kg, still significantly above toxic level (> 5mg/kg). Furthermore, contrary to conventional believing, the major mercury form in current soils of this particular area of floodplain of LEFPC is mainly in non-cinnabar mercury bound in clay minerals (after decades of remediation). The floodplains can act both as a medium-term sink and as long-term sources. Native North

  7. Sources of acid and metals from the weathering of the Dinero waste pile, Lake Fork watershed, Leadville, Colorado

    Science.gov (United States)

    Diehl, S.F.; Hageman, Phil L.; Smith, Kathleen S.; Herron, J.T.; Desborough, G.A.

    2005-01-01

    Two trenches were dug into the south Dinero mine-waste pile near Leadville, Colorado, to study the weathering of rock fragments and the mineralogic sources of metal contaminants in the surrounding wetland and Lake Fork Watershed. Water seeping from the base of the south Dinero waste-rock pile was pH 2.9, whereas leachate from a composite sample of the rock waste was pH 3.3. The waste pile was mostly devoid of vegetation, open to infiltration of precipitation, and saturated at the base because of placement in the wetland. The south mine-waste pile is composed of poorly sorted material, ranging from boulder-size to fine-grained rock fragments. The trenches showed both matrix-supported and clast-supported zones, with faint horizontal color banding, suggesting zonation of Fe oxides. Secondary minerals such as jarosite and gypsum occurred throughout the depth of the trenches. Infiltration of water and transport of dissolved material through the pile is evidenced by optically continuous secondary mineral deposits that fill or line voids. Iron-sulfate material exhibits microlaminations with shrinkage cracking and preferential dissolution of microlayers that evidence drying and wetting events. In addition to fluids, submicron-sized to very fine-grained particles such as jarosite are transported through channel ways in the pile. Rock fragments are coated with a mixture of clay, jarosite, and manganese oxides. Dissolution of minerals is a primary source of metals. Skeletal remnants of grains, outlined by Fe-oxide minerals, are common. Potassium jarosite is the most abundant jarosite phase, but Pb-and Ag-bearing jarosite are common. Grain-sized clusters of jarosite suggest that entire sulfide grains were replaced by very fine-grained jarosite crystals. The waste piles were removed from the wetland and reclaimed upslope in 2003. This was an opportunity to test methods to identify sources of acid and metals and metal transport processes within a waste pile. A series of

  8. Quantitative farm-to-fork human norovirus exposure assessment of individually quick frozen raspberries and raspberry puree.

    Science.gov (United States)

    Jacxsens, L; Stals, A; De Keuckelaere, A; Deliens, B; Rajkovic, A; Uyttendaele, M

    2017-02-02

    package containing approximately 115 berries. This farm-to-fork model is a useful tool for evaluating NoV mitigation strategies in the soft red fruit supply chain. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. A retrospective study of the chemical analysis cost for the remediation of Lower East Fork Poplar Creek, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Klatt, L.N.

    1998-06-01

    A retrospective study of the remediation of Lower East Fork Poplar Creek (LEFPC) in Oak Ridge, Tennessee was completed. The study was conducted by reviewing the public Comprehensive Environmental Response, Compensation, and Liability Act record documents associated with the remediation of LEFPC and through discussions with the project staff involved or familiar with the project. The remediation took place in two phases. The first phase involved the excavation of about 5,560 yd 3 of soil at the National Oceanic and Atmospheric Administration (NOAA) locations in 1996. The second phase involved the excavation of 39,200 yd 3 at another NOAA location and at the Bruner location in 1997. For the entire project (remedial investigation through cleanup), a total of 7,708 samples (1 sample for each 5.8 yd 3 of soil remediated) were analyzed for mercury. The project obtained special regulatory approval to use two methods for the determination of mercury in soils that are not part of the Resource Conservation and Recovery Act SW-846 methods manual. The mercury analysis cost was $678,000, which represents 9.6% of the cleanup cost. During the cleanup phase of the project, an on-site laboratory was used. The estimated cost savings that the on-site laboratory provided fall into two categories: direct reduction of costs associated with chemical analysis and sample shipment totaling approximately $38,000, which represents a 5.3% savings relative to the estimated cost of using an off-site laboratory, and savings in the amount of $890,000 (12.5% of the $7.1 M cleanup cost), associated with expediting execution of the cleanup work by providing rapid (< 3 hours) sample result turnaround time. The manner in which the analytical services were procured for the LEFPC project suggest that the development of new chemical analysis technology must address deployment, performance, regulatory, robustness, reliability, and business appropriateness factors if the technology is to be used in

  10. Hydrology and model of North Fork Solomon River Valley, Kirwin Dam to Waconda Lake, north-central Kansas

    Science.gov (United States)

    Jorgensen, Donald G.; Stullken, Lloyd E.

    1981-01-01

    The alluvial valley of the North Fork Solomon River is an important agricultural area. Reservoir releases diverted below Kirwin Dam are the principal source of irrigation water. During the 1970'S, severe water shortages occurred in Kirwin Reservoir and other nearby reservoirs as a result of an extended drought. Some evidence indicates that surface-water shortages may have been the result of a change in the rainfall-runoff relationship. Examination of the rainfall-runoff relationship shows no apparent trend from 1951 to 1968, but annual records from 1969 to 1976 indicate that deficient rainfall occurred during 6 of the 8 years. Ground water from the alluvial aquifer underlying the river valley also is used extensively for irrigation. Utilization of ground water for irrigation greatly increased from about 200 acre-feet in 1955 to about 12,300 acre-feet in 1976. Part of the surface water diverted for irrigation has percolated downward into the aquifer raising the ground-water level. Ground-water storage in the aquifer increased from 230,000 acre-feet in 1946 to 275,000 acre-feet in 1976-77. A digital model was used to simulate the steady-state conditions in the aquifer prior to closure of Kirwin Dam. Model results indicated that precipitation was the major source of recharge to the aquifer. The effective recharge, or gain from precipitation minus evapotranspiration, was about 11,700 acre-feet per year. The major element of discharge from the aquifer was leakage to the river. The simulated net leakage (leakage to the river minus leakage from the river) was about 11,500 acre-feet per year. The simulated value is consistent with the estimated gain in base flow of the river within the area modeled. Measurements of seepage used to determine gain and loss to the stream were made twice during 1976. Based on these measurements and on base-flow periods identified from hydrographs, it was estimated that the ground-water discharge to the stream has increased about 4,000 acre

  11. Development of a quartz tuning-fork-based force sensor for measurements in the tens of nanoNewton force range during nanomanipulation experiments

    Energy Technology Data Exchange (ETDEWEB)

    Oiko, V. T. A., E-mail: oiko@ifi.unicamp.br; Rodrigues, V.; Ugarte, D. [Instituto de Física “Gleb Wataghin,” Univ. Estadual de Campinas (UNICAMP), Campinas 13083-859 (Brazil); Martins, B. V. C. [Department of Physics, University of Alberta, Edmonton, Alberta T6G 2R3 (Canada); Silva, P. C. [Laboratório Nacional de Nanotecnologia, CNPEM, Campinas 13083-970 (Brazil)

    2014-03-15

    Understanding the mechanical properties of nanoscale systems requires new experimental and theoretical tools. In particular, force sensors compatible with nanomechanical testing experiments and with sensitivity in the nN range are required. Here, we report the development and testing of a tuning-fork-based force sensor for in situ nanomanipulation experiments inside a scanning electron microscope. The sensor uses a very simple design for the electronics and it allows the direct and quantitative force measurement in the 1–100 nN force range. The sensor response is initially calibrated against a nN range force standard, as, for example, a calibrated Atomic Force Microscopy cantilever; subsequently, applied force values can be directly derived using only the electric signals generated by the tuning fork. Using a homemade nanomanipulator, the quantitative force sensor has been used to analyze the mechanical deformation of multi-walled carbon nanotube bundles, where we analyzed forces in the 5–40 nN range, measured with an error bar of a few nN.

  12. Flood-inundation maps for South Fork Peachtree Creek from the Brockett Road bridge to the Willivee Drive bridge, DeKalb County, Georgia

    Science.gov (United States)

    Musser, Jonathan W.

    2015-10-14

    Digital flood-inundation maps for a 5.3-mile reach of South Fork Peachtree Creek that extends from about 500 feet above the Brockett Road bridge to the Willivee Drive bridge were developed by the U.S. Geological Survey (USGS) in cooperation with DeKalb County, Georgia. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the USGS streamgage at South Fork Peachtree at Casa Drive, near Clarkston, Georgia (02336152). Real-time stage information from this USGS streamgage may be obtained at http://waterdata.usgs.gov/ and can be used in conjunction with these maps to estimate near real-time areas of inundation. The National Weather Service (NWS) is incorporating results from this study into the Advanced Hydrologic Prediction Service (AHPS) flood-warning system (http://water.weather.gov/ahps/).

  13. Calendar year 1994 groundwater quality report for the Upper East Fork Poplar Creek Hydrogeologic Regime, Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-10-01

    This groundwater quality report (GWQR) contains an evaluation of the groundwater quality data obtained during the 1994 calendar year (CY) at several waste-management facilities and a petroleum fuel underground storage tank (UST) site at the US Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. These sites lie within the boundaries of the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), which is one of three hydrogeologic regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant. The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability (HSEA) Organization manages the groundwater monitoring activities in each regime under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). The purpose of the GWPP is to characterize the hydrogeology and to monitor groundwater quality at the Y-12 Plant and surrounding area to ensure protection of local groundwater resources in accordance with federal, state, and local regulations, DOE Orders, and Lockheed Martin Energy Systems, Inc. (Energy Systems) corporate policy

  14. Double-Forked Artifice

    DEFF Research Database (Denmark)

    Elias, Camelia

    2012-01-01

    to ignite established ideas, and blow them to pieces with his own dynamite. Quite literally, can an elastic set things on fire, survive the fire? Can a truth be final even when it is elastic? What does it mean to write, for Nietzsche, when writing presents itself as a sin against silence, as a fire...

  15. A Novel Rrm3 Function in Restricting DNA Replication via an Orc5-Binding Domain Is Genetically Separable from Rrm3 Function as an ATPase/Helicase in Facilitating Fork Progression

    DEFF Research Database (Denmark)

    Syed, Salahuddin; Madsen, Claus Desler; Rasmussen, Lene J.

    2016-01-01

    hydroxyurea. This novel Rrm3 function is independent of its established role as an ATPase/helicase in facilitating replication fork progression through polymerase blocking obstacles. Using quantitative mass spectrometry and genetic analyses, we find that the homologous recombination factor Rdh54 and Rad5...

  16. Post-closure permit application for the Upper East Fork Poplar Creek hydrogeologic regime at the Y-12 Plant: New Hope Pond and Eastern S-3 ponds plume. Revision 2

    International Nuclear Information System (INIS)

    1995-02-01

    The intent of this Post-Closure, Permit Application (PCPA) is to satisfy the post-closure permitting requirements of the Tennessee Department of Environment and Conservation (TDEC) Rule 1200-1-11. This application is for the entire Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), which is within the Bear Creek Valley (BCV). This PCPA has been prepared to include the entire East Fork Regime because, although there are numerous contaminant sources within the regime, the contaminant plumes throughout the East Fork Regime have coalesced and can no longer be distinguished as separate plumes. This PCPA focuses on two recognized Resource Conservation and Recovery Act (RCRA) interim status units: New Hope Pond (NHP) and the eastern S-3 Ponds plume. This PCPA presents data from groundwater assessment monitoring throughout the regime, performed since 1986. Using this data, this PCPA demonstrates that NHP is not a statistically discernible source of groundwater contaminants and that sites upgradient of NHP are the likely sources of groundwater contamination seen in the NHP vicinity. As such, this PCPA proposes a detection monitoring program to replace the current assessment monitoring program for NHP

  17. Water and Proppant Requirements and Water Production Associated with Undiscovered Petroleum in the Bakken and Three Forks Formations, North Dakota and Montana, USA

    Science.gov (United States)

    Haines, S. S.; Varela, B. A.; Thamke, J.; Hawkins, S. J.; Gianoutsos, N. J.; Tennyson, M. E.

    2017-12-01

    Water is used for several stages of oil and gas production, in particular for hydraulic fracturing that is typically used during production of petroleum from low-permeability shales and other rock types (referred to as "continuous" petroleum accumulations). Proppant, often sand, is also consumed during hydraulic fracturing. Water is then produced from the reservoir along with the oil and gas, representing either a disposal consideration or a possible source of water for further petroleum development or other purposes. The U.S. Geological Survey (USGS) has developed an approach for regional-scale estimation of these water and proppant quantities in order to provide an improved understanding of possible impacts and to help with planning and decision-making. Using the new methodology, the USGS has conducted a quantitative assessment of water and proppant requirements, and water production volumes, associated with associated with possible future production of undiscovered petroleum resources in the Bakken and Three Forks Formations, Williston Basin, USA. This water and proppant assessment builds directly from the 2013 USGS petroleum assessment for the Bakken and Three Forks Formations. USGS petroleum assessments incorporate all available geologic and petroleum production information, and include the definition of assessment units (AUs) that specify the geographic regions and geologic formations for the assessment. The 2013 petroleum assessment included 5 continuous AUs for the Bakken Formation and one continuous AU for the Three Forks Formation. The assessment inputs are defined probabilistically, and a Monte Carlo approach provides outputs that include uncertainty bounds. We can summarize the assessment outputs with the mean values of the associated distributions. The mean estimated total volume of water for well drilling and cement for all six continuous AUs is 5.9 billion gallons, and the mean estimated volume of water for hydraulic fracturing for all AUs is 164

  18. High-resolution digital elevation model of Mount St. Helens crater and upper North Fork Toutle River basin, Washington, based on an airborne lidar survey of September 2009

    Science.gov (United States)

    Mosbrucker, Adam

    2014-01-01

    The lateral blast, debris avalanche, and lahars of the May 18th, 1980, eruption of Mount St. Helens, Washington, dramatically altered the surrounding landscape. Lava domes were extruded during the subsequent eruptive periods of 1980–1986 and 2004–2008. More than three decades after the emplacement of the 1980 debris avalanche, high sediment production persists in the North Fork Toutle River basin, which drains the northern flank of the volcano. Because this sediment increases the risk of flooding to downstream communities on the Toutle and Cowlitz Rivers, the U.S. Army Corps of Engineers (USACE), under the direction of Congress to maintain an authorized level of flood protection, built a sediment retention structure on the North Fork Toutle River in 1989 to help reduce this risk and to prevent sediment from clogging the shipping channel of the Columbia River. From September 16–20, 2009, Watershed Sciences, Inc., under contract to USACE, collected high-precision airborne lidar (light detection and ranging) data that cover 214 square kilometers (83 square miles) of Mount St. Helens and the upper North Fork Toutle River basin from the sediment retention structure to the volcano's crater. These data provide a digital dataset of the ground surface, including beneath forest cover. Such remotely sensed data can be used to develop sediment budgets and models of sediment erosion, transport, and deposition. The U.S. Geological Survey (USGS) used these lidar data to develop digital elevation models (DEMs) of the study area. DEMs are fundamental to monitoring natural hazards and studying volcanic landforms, fluvial and glacial geomorphology, and surface geology. Watershed Sciences, Inc., provided files in the LASer (LAS) format containing laser returns that had been filtered, classified, and georeferenced. The USGS produced a hydro-flattened DEM from ground-classified points at Castle, Coldwater, and Spirit Lakes. Final results averaged about five laser last

  19. Water temperature effects from simulated dam operations and structures in the Middle Fork Willamette River, western Oregon

    Science.gov (United States)

    Buccola, Norman L.; Turner, Daniel F.; Rounds, Stewart A.

    2016-09-14

    Significant FindingsStreamflow and water temperature in the Middle Fork Willamette River (MFWR), western Oregon, have been regulated and altered since the construction of Lookout Point, Dexter, and Hills Creek Dams in 1954 and 1961, respectively. Each year, summer releases from the dams typically are cooler than pre-dam conditions, with the reverse (warmer than pre-dam conditions) occurring in autumn. This pattern has been detrimental to habitat of endangered Upper Willamette River (UWR) Chinook salmon (Oncorhynchus tshawytscha) and UWR winter steelhead (O. mykiss) throughout multiple life stages. In this study, scenarios testing different dam-operation strategies and hypothetical dam-outlet structures were simulated using CE-QUAL-W2 hydrodynamic/temperature models of the MFWR system from Hills Creek Lake (HCR) to Lookout Point (LOP) and Dexter (DEX) Lakes to explore and understand the efficacy of potential flow and temperature mitigation options.Model scenarios were run in constructed wet, normal, and dry hydrologic calendar years, and designed to minimize the effects of Hills Creek and Lookout Point Dams on river temperature by prioritizing warmer lake surface releases in May–August and cooler, deep releases in September–December. Operational scenarios consisted of a range of modified release rate rules, relaxation of power-generation constraints, variations in the timing of refill and drawdown, and maintenance of different summer maximum lake levels at HCR and LOP. Structural scenarios included various combinations of hypothetical floating outlets near the lake surface and hypothetical new outlets at depth. Scenario results were compared to scenarios using existing operational rules that give temperature management some priority (Base), scenarios using pre-2012 operational rules that prioritized power generation over temperature management (NoBlend), and estimated temperatures from a without-dams condition (WoDams).Results of the tested model scenarios led

  20. Continuous water-quality monitoring and regression analysis to estimate constituent concentrations and loads in the Red River of the North at Fargo and Grand Forks, North Dakota, 2003-12

    Science.gov (United States)

    Galloway, Joel M.

    2014-01-01

    The Red River of the North (hereafter referred to as “Red River”) Basin is an important hydrologic region where water is a valuable resource for the region’s economy. Continuous water-quality monitors have been operated by the U.S. Geological Survey, in cooperation with the North Dakota Department of Health, Minnesota Pollution Control Agency, City of Fargo, City of Moorhead, City of Grand Forks, and City of East Grand Forks at the Red River at Fargo, North Dakota, from 2003 through 2012 and at Grand Forks, N.Dak., from 2007 through 2012. The purpose of the monitoring was to provide a better understanding of the water-quality dynamics of the Red River and provide a way to track changes in water quality. Regression equations were developed that can be used to estimate concentrations and loads for dissolved solids, sulfate, chloride, nitrate plus nitrite, total phosphorus, and suspended sediment using explanatory variables such as streamflow, specific conductance, and turbidity. Specific conductance was determined to be a significant explanatory variable for estimating dissolved solids concentrations at the Red River at Fargo and Grand Forks. The regression equations provided good relations between dissolved solid concentrations and specific conductance for the Red River at Fargo and at Grand Forks, with adjusted coefficients of determination of 0.99 and 0.98, respectively. Specific conductance, log-transformed streamflow, and a seasonal component were statistically significant explanatory variables for estimating sulfate in the Red River at Fargo and Grand Forks. Regression equations provided good relations between sulfate concentrations and the explanatory variables, with adjusted coefficients of determination of 0.94 and 0.89, respectively. For the Red River at Fargo and Grand Forks, specific conductance, streamflow, and a seasonal component were statistically significant explanatory variables for estimating chloride. For the Red River at Grand Forks, a time

  1. An archaeological reconnaissance of a 14 mile section of the East Fork Poplar Creek for the Environmental Restoration Project, Anderson and Roane Counties, Tennessee

    International Nuclear Information System (INIS)

    DuVall, G.D.

    1993-01-01

    At the request of the US Army Corps of Engineers, Nashville District, Nashville, Tennessee, an archaeological reconnaissance of the potential impact areas of the Environmental Restoration Project (ERP) along the East Fork Poplar Creek was conducted during the period December 16, 1991, and March 3, 1992. The reconnaissance was conducted in response to environmental evaluations as a result of the accidental spillage of approximately 293,000 pounds of mercury, radionuclides, heavy metals and other compounds. The reconnaissance to assess adverse impacts to cultural resources located within the boundaries of Federally-licensed, permitted, funded or assisted projects was conducted in compliance with the National Historic Preservation Act of 1966, National Environmental Policy Act of 1969 and Executive Order 11593

  2. Age and growth of the Duckbill Catfish (Sorubim cf. lima in the Pantanal

    Directory of Open Access Journals (Sweden)

    J. M. F. Penha

    Full Text Available The Duckbill Catfish, Sorubim lima, is a predator of large South American rivers. The age and growth of S. lima were studied based on the pectoral fin-spines of samples collected from the Cuiabá River, Pantanal. The samples were taken from commercial and experimental hook-and-line fishing. An analysis of the marginal increment suggests that the growth rings are formed once a year during the dry season, from July to September (ANOVA type I: F = 4.183; g.l. = 3 and 104; p = 0.008. The estimate of the parameters that describe von Bertalanffy's growth curve by nonlinear regression of the observed lengths in the age were: L¥ = 56.0 cm (fork length; k = 0.245 year-1; t o = -2.605 years. The animals were estimated to have a life span of 9.6 years. The findings indicate that the fork length is a good predictor of the age of individuals of this fish species.

  3. Remedial investigation work plan for the Upper East Fork Poplar Creek characterization area, Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1995-09-01

    The Oak Ridge Y-12 Plant, located within the Oak Ridge Reservation (ORR), is owned by the US Department of Energy (DOE) and managed by Lockheed Martin Energy Systems, Inc. The entire ORR was placed on the National Priorities List (NPL) of CERCLA sites in November 1989. Following CERCLA guidelines, sites under investigation require a remedial investigation (RI) to define the nature and extent of contamination, evaluate the risks to public health and the environment, and determine the goals for a feasibility study (FS) of potential remedial actions. The need to complete RIs in a timely manner resulted in the establishment of the Upper East Fork Poplar Creek (UEFPC) Characterization Area (CA) and the Bear Creek CA. The CA approach considers the entire watershed and examines all appropriate media within it. The UEFPC CA, which includes the main Y-12 Plant area, is an operationally and hydrogeologically complex area that contains numerous contaminants and containment sources, as well as ongoing industrial and defense-related activities. The UEFPC CA also is the suspected point of origin for off-site groundwater and surface-water contamination. The UEFPC CA RI also will address a carbon-tetrachloride/chloroform-dominated groundwater plume that extends east of the DOE property line into Union Valley, which appears to be connected with springs in the valley. In addition, surface water in UEFPC to the Lower East Fork Poplar Creek CA boundary will be addressed. Through investigation of the entire watershed as one ''site,'' data gaps and contaminated areas will be identified and prioritized more efficiently than through separate investigations of many discrete units.

  4. Spatial and temporal distribution of bull trout (Salvelinus confluentus)-size fish near the floating surface collector in the North Fork Reservoir, Oregon, 2016

    Science.gov (United States)

    Adams, Noah S.; Smith, Collin D.

    2017-06-26

    Acoustic cameras were used to assess the behavior and abundance of bull trout (Salvelinus confluentus)-size fish at the entrance to the North Fork Reservoir juvenile fish floating surface collector (FSC). The purpose of the FSC is to collect downriver migrating juvenile salmonids at the North Fork Dam, and safely route them around the hydroelectric projects. The objective of the acoustic camera component of this study was to assess the behaviors of bull trout-size fish observed near the FSC, and to determine if the presence of bull trout-size fish influenced the collection or abundance of juvenile salmonids. Acoustic cameras were deployed near the surface and floor of the entrance to the FSC. The acoustic camera technology was an informative tool for assessing abundance and spatial and temporal behaviors of bull trout-size fish near the entrance of the FSC. Bull trout-size fish were regularly observed near the entrance, with greater abundances on the deep camera than on the shallow camera. Additionally, greater abundances were observed during the hours of sunlight than were observed during the night. Behavioral differences also were observed at the two depths, with surface fish traveling faster and straighter with more directed movement, and fish observed on the deep camera generally showing more milling behavior. Modeling potential predator-prey interactions and influences using collected passive integrated transponder (PIT) -tagged juvenile salmonids proved largely unpredictable, although these fish provided relevant timing and collection information. Overall, the results indicate that bull trout-size fish are present near the entrance of the FSC, concomitant with juvenile salmonids, and their abundances and behaviors indicate that they may be drawn to the entrance of the FSC because of the abundance of prey-sized fish.

  5. Preliminary assessment of aggradation potential in the North Fork Stillaguamish River downstream of the State Route 530 landslide near Oso, Washington

    Science.gov (United States)

    Magirl, Christopher S.; Keith, Mackenzie K.; Anderson, Scott W.; O'Connor, Jim; Robert Aldrich,; Mastin, Mark C.

    2015-12-28

    On March 22, 2014, the State Route 530 Landslide near Oso, Washington, traveled almost 2 kilometers (km), destroyed more than 40 structures, and impounded the North Fork Stillaguamish River to a depth of 8 meters (m) and volume of 3.3×106 cubic meters (m3). The landslide killed 43 people. After overtopping and establishing a new channel through the landslide, the river incised into the landslide deposit over the course of 10 weeks draining the impoundment lake and mobilizing an estimated 280,000±56,000 m3 of predominantly sand-sized and finer sediment. During the first 4 weeks after the landslide, this eroded sediment caused downstream riverbed aggradation of 1–2 m within 1 km of the landslide and 0.4 m aggradation at Whitman Road Bridge, 3.5 km downstream. Winter high flows in 2014–15 were anticipated to mobilize an additional 220,000±44,000 m3 of sediment, potentially causing additional aggradation and exacerbating flood risk downstream of the landslide. Analysis of unit stream power and bed-material transport capacity along 35 km of the river corridor indicated that most fine-grained sediment will transport out of the North Fork Stillaguamish River, although some localized additional aggradation was possible. This new aggradation was not likely to exceed 0.1 m except in reaches within a few kilometers downstream of the landslide, where additional aggradation of up to 0.5 m is possible. Alternative river response scenarios, including continued mass wasting from the landslide scarp, major channel migration or avulsion, or the formation of large downstream wood jams, although unlikely, could result in reaches of significant local aggradation or channel change.

  6. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage.

    Science.gov (United States)

    Waraky, Ahmed; Lin, Yingbo; Warsito, Dudi; Haglund, Felix; Aleem, Eiman; Larsson, Olle

    2017-11-03

    We have previously shown that the insulin-like growth factor 1 receptor (IGF-1R) translocates to the cell nucleus, where it binds to enhancer-like regions and increases gene transcription. Further studies have demonstrated that nuclear IGF-1R (nIGF-1R) physically and functionally interacts with some nuclear proteins, i.e. the lymphoid enhancer-binding factor 1 (Lef1), histone H3, and Brahma-related gene-1 proteins. In this study, we identified the proliferating cell nuclear antigen (PCNA) as a nIGF-1R-binding partner. PCNA is a pivotal component of the replication fork machinery and a main regulator of the DNA damage tolerance (DDT) pathway. We found that IGF-1R interacts with and phosphorylates PCNA in human embryonic stem cells and other cell lines. In vitro MS analysis of PCNA co-incubated with the IGF-1R kinase indicated tyrosine residues 60, 133, and 250 in PCNA as IGF-1R targets, and PCNA phosphorylation was followed by mono- and polyubiquitination. Co-immunoprecipitation experiments suggested that these ubiquitination events may be mediated by DDT-dependent E2/E3 ligases ( e.g. RAD18 and SHPRH/HLTF). Absence of IGF-1R or mutation of Tyr-60, Tyr-133, or Tyr-250 in PCNA abrogated its ubiquitination. Unlike in cells expressing IGF-1R, externally induced DNA damage in IGF-1R-negative cells caused G 1 cell cycle arrest and S phase fork stalling. Taken together, our results suggest a role of IGF-1R in DDT. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  7. Geohydrologic Investigations and Landscape Characteristics of Areas Contributing Water to Springs, the Current River, and Jacks Fork, Ozark National Scenic Riverways, Missouri

    Science.gov (United States)

    Mugel, Douglas N.; Richards, Joseph M.; Schumacher, John G.

    2009-01-01

    The Ozark National Scenic Riverways (ONSR) is a narrow corridor that stretches for approximately 134 miles along the Current River and Jacks Fork in southern Missouri. Most of the water flowing in the Current River and Jacks Fork is discharged to the rivers from springs within the ONSR, and most of the recharge area of these springs is outside the ONSR. This report describes geohydrologic investigations and landscape characteristics of areas contributing water to springs and the Current River and Jacks Fork in the ONSR. The potentiometric-surface map of the study area for 2000-07 shows that the groundwater divide extends beyond the surface-water divide in some places, notably along Logan Creek and the northeastern part of the study area, indicating interbasin transfer of groundwater between surface-water basins. A low hydraulic gradient occurs in much of the upland area west of the Current River associated with areas of high sinkhole density, which indicates the presence of a network of subsurface karst conduits. The results of a low base-flow seepage run indicate that most of the discharge in the Current River and Jacks Fork was from identified springs, and a smaller amount was from tributaries whose discharge probably originated as spring discharge, or from springs or diffuse groundwater discharge in the streambed. Results of a temperature profile conducted on an 85-mile reach of the Current River indicate that the lowest average temperatures were within or downstream from inflows of springs. A mass-balance on heat calculation of the discharge of Bass Rock Spring, a previously undescribed spring, resulted in an estimated discharge of 34.1 cubic feet per second (ft3/s), making it the sixth largest spring in the Current River Basin. The 13 springs in the study area for which recharge areas have been estimated accounted for 82 percent (867 ft3/s of 1,060 ft3/s) of the discharge of the Current River at Big Spring during the 2006 seepage run. Including discharge from

  8. Ageing Polulations

    DEFF Research Database (Denmark)

    Christiansen, Terkel; Lauridsen, Jørgen Trankjær; Bech, Mickael

    2013-01-01

    An ageing society is characterised by an increasing median age of the population. The purpose of this chapter is to document the existing knowledge about the association between population ageing and health care expenditure, and to supplement this overview by a summary of our original research...... is that ageing as such can be expected only to a cause modest increase in health care expenditure per capita in the future. This conclusion is supported by our own empirical study, based on 15 EU countries....

  9. Skin Aging

    Science.gov (United States)

    Your skin changes as you age. You might notice wrinkles, age spots and dryness. Your skin also becomes thinner and loses fat, making it ... heal, too. Sunlight is a major cause of skin aging. You can protect yourself by staying out ...

  10. Communication & Aging.

    Science.gov (United States)

    Arnold, William E.

    This extensive bibliography contains more than 1,800 entries about communication and aging. The citations include journal articles, unpublished papers, speeches, dissertations, research studies, and books that relate aging and the aged to a variety of topics, including the following: physiological deterioration, socialization, political…

  11. Selected trace-element and synthetic-organic compound data for streambed sediment from the Clark Fork-Pend Oreille and Spokane River basins, Montana, Idaho, and Washington, 1998

    Science.gov (United States)

    Beckwith, Michael A.

    2002-01-01

    Streambed-sediment samples were collected at 22 sites during the summer of 1998 as part of the U.S. Geological Survey National Water-Quality Assessment Program. Sampling sites in the Clark Fork-Pend Oreille and Spokane River basins represented a wide range of environmental conditions including pristine mountain streams and large rivers affected by mining-related and urban activities. Samples were analyzed for 45 inorganic major and trace elements, 109 syn­thetic organic compounds, and carbon. This report pre­sents the selected results of streambed-sediment sampling from the Clark Fork-Pend Oreille and Spo­kane River basins in Montana, Idaho, and Washington.

  12. Active ageing

    Directory of Open Access Journals (Sweden)

    Frode F. Jacobsen

    2017-09-01

    Full Text Available Background: The concept of active ageing has been gaining prominence in the Nordic countries and beyond. This has been reflected in policy papers in Norway and other Nordic nations. Aims: The aim of this article is to analyse the topic of active ageing in five Norwegian White Papers (2002 to 2015 and discuss those policy documents in context of relevant research literature. Methods: A qualitative document analyses is employed focusing on how active ageing, and ageing in general, is described and which concepts are employed. No ethical approval was needed. Findings: The general theme of ageing and the specific theme of active ageing are increasingly prominent in the Norwegian White Papers studied. In all documents, some assumptions regarding ageing and active ageing seem implicit, such as independence being more important than (interdependence. ‘Productive’ activities like participation in working life are stressed, while others, like reading, watching TV or watching children playing in the street, are ignored. Conclusions: The policy documents demonstrate that the topic of active ageing is growing in importance. The documents increasingly seem to stress ‘productive’ activities – those related to working life, voluntary work or sports and physical training. They exclude activities that are meaningful for many older people, like watching their grandchildren play or reading books. Implications for practice: Practitioners in older people’s care could consider reflecting on: Government documents dealing with their own practice The prevalent concept of active ageing The trend of active ageing as a facilitating or hindering factor for good care work How present discourse on active ageing may influence their attitude towards frail older persons How they wish to relate to active ageing in their own practice

  13. Calendar year 1995 groundwater quality report for the Upper East Fork Poplar Creek Hydrogeologic Regime Y-12 Plant, Oak Ridge Tennessee. 1995 Groundwater quality data interpretations and proposed program modifications

    International Nuclear Information System (INIS)

    1996-08-01

    This groundwater quality report (GWQR) contains an evaluation of the groundwater quality data obtained during the 1995 calendar year (CY) at several waste management facilities associated with the US Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee. These sites lie within the boundaries of the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime), which is one of three hydrogeologic regimes defined for the purposes of groundwater quality monitoring at the Y-12 Plant. The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability (HSEA) Organization manages the groundwater monitoring activities in each regime under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). The purpose of the GWPP is to characterize the hydrogeology and to monitor groundwater quality at the Y-12 Plant and surrounding area to ensure protection of local groundwater resources in accordance with federal, state, and local regulations, DOE Orders, and Lockheed Martin Energy Systems, Inc. (Energy Systems) corporate policy. The annual GWQR for the East Fork Regime is completed in two parts. Part I consists primarily of data appendices and serves as a reference for the groundwater quality data obtained each CY under the lead of the Y-12 Plant GWPP. Because it contains information needed to comply with reporting requirements of Resource Conservation and Recovery Act (RCRA) interim status assessment monitoring, the Part I GWQR is submitted to the Tennessee Department of Environment and Conservation (TDEC) by the RCRA reporting deadline (March 1 of the following CY); Energy Systems submitted the 1995 Part I GWQR for the East Fork Regime to the TDEC in February 1996. Part 2 (this report) contains an evaluation of the data with respect to regime-wide groundwater quality

  14. Aging research

    International Nuclear Information System (INIS)

    Ross, D.F. Jr.

    1988-01-01

    The USNRC Office of Nuclear Regulatory Research has developed a program for nuclear plant aging research (NPAR) to achieve an understanding of nuclear plant aging, its potential effects on safety, and methods for its detection and mitigation, sufficient for addressing safety and regulatory issues and supporting regulatory decisions on issues. Specifically, the NRC has aggressive research and regulatory programs associated with aging effects on piping, steam generators, containments, structures, and electrical and mechanical systems and components. In addition to safety assessment for the original license period for nuclear power plants, this aging information will be extremely useful in providing technical bases for efficient and effective regulation associated with possible license extension. This paper discusses the major activities of USNRC sponsored aging research program and recommends an approach to manage and handle aging at nuclear power plants

  15. Groundwater Protection Program Calendar Year 1998 Evaluation of Groundwater Quality Data for the Upper East Fork Poplar Creek Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    None

    1999-01-01

    This report presents an evaluation of the water quality monitoring data obtained by the Y-12 Plant Groundwater Protection Program (GWPP) in the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) during calendar year (CY) 1998. The East Fork Regime contains many confirmed and potential sources of groundwater and surface water contamination associated with the U.S. Department of Energy (DOE) Oak Ridge Y-12 Plant. Applicable provisions of DOE Order 5400.1A - General Environmental Protection Program - require evaluation of groundwater and surface water quality near the Y-12 Plant to: (1) gauge groundwater quality in areas that are, or could be, affected by plant operations, (2) determine the quality of surface water and groundwater where contaminants are most likely to migrate beyond the DOE Oak Ridge Reservation (ORR) property line, and (3) identify and characterize long-term trends in groundwater quality at the Y-12 Plant. The following sections of this report contain relevant background information (Section 2.0); describe the results of the respective data evaluations required under DOE Order 5400.1A (Section 3.0); summarize significant findings of each evaluation (Section 4.0); and list the technical reports and regulatory documents cited for more detailed information (Section 5.0). All of the illustrations (maps and trend graphs) and data summary tables referenced in each section are presented in Appendix A and Appendix B, respectively

  16. Groundwater Protection Program Calendar Year 1998 Evaluation of Groundwater Quality Data for the Upper East Fork Poplar Creek Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    None

    1999-09-01

    This report presents an evaluation of the water quality monitoring data obtained by the Y-12 Plant Groundwater Protection Program (GWPP) in the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime) during calendar year (CY) 1998. The East Fork Regime contains many confirmed and potential sources of groundwater and surface water contamination associated with the U.S. Department of Energy (DOE) Oak Ridge Y-12 Plant. Applicable provisions of DOE Order 5400.1A - General Environmental Protection Program - require evaluation of groundwater and surface water quality near the Y-12 Plant to: (1) gauge groundwater quality in areas that are, or could be, affected by plant operations, (2) determine the quality of surface water and groundwater where contaminants are most likely to migrate beyond the DOE Oak Ridge Reservation (ORR) property line, and (3) identify and characterize long-term trends in groundwater quality at the Y-12 Plant. The following sections of this report contain relevant background information (Section 2.0); describe the results of the respective data evaluations required under DOE Order 5400.1A (Section 3.0); summarize significant findings of each evaluation (Section 4.0); and list the technical reports and regulatory documents cited for more detailed information (Section 5.0). All of the illustrations (maps and trend graphs) and data summary tables referenced in each section are presented in Appendix A and Appendix B, respectively.

  17. Flood-inundation maps for a 9.1-mile reach of the Coast Fork Willamette River near Creswell and Goshen, Lane County, Oregon

    Science.gov (United States)

    Hess, Glen W.; Haluska, Tana L.

    2016-04-13

    Digital flood-inundation maps for a 9.1-mile reach of the Coast Fork Willamette River near Creswell and Goshen, Oregon, were developed by the U.S. Geological Survey (USGS) in cooperation with the U.S. Army Corps of Engineers (USACE). The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding corresponding to selected stages at the USGS streamgage at Coast Fork Willamette River near Goshen, Oregon (14157500), at State Highway 58. Current stage at the streamgage for estimating near-real-time areas of inundation may be obtained at http://waterdata.usgs.gov/or/nwis/uv/?site_no=14157500&PARAmeter_cd=00065,00060. In addition, the National Weather Service (NWS) forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation.In this study, areas of inundation were provided by USACE. The inundated areas were developed from flood profiles simulated by a one-dimensional unsteady step‑backwater hydraulic model. The profiles were checked by the USACE using documented high-water marks from a January 2006 flood. The model was compared and quality assured using several other methods. The hydraulic model was then used to determine eight water-surface profiles at various flood stages referenced to the streamgage datum and ranging from 11.8 to 19.8 ft, approximately 2.6 ft above the highest recorded stage at the streamgage (17.17 ft) since 1950. The intervals between stages are variable and based on annual exceedance probability discharges, some of which approximate NWS action stages.The areas of inundation and water depth grids provided to USGS by USACE were used to create interactive flood‑inundation maps. The availability of these maps with current stage from USGS streamgage and forecasted stream stages from the NWS provide emergency management

  18. Successful ageing

    DEFF Research Database (Denmark)

    Kusumastuti, Sasmita; Derks, Marloes G. M.; Tellier, Siri

    2016-01-01

    BACKGROUND: Ageing is accompanied by an increased risk of disease and a loss of functioning on several bodily and mental domains and some argue that maintaining health and functioning is essential for a successful old age. Paradoxically, studies have shown that overall wellbeing follows a curvili...

  19. Ageing genes

    DEFF Research Database (Denmark)

    Rattan, Suresh

    2018-01-01

    The idea of gerontogenes is in line with the evolutionary explanation of ageing as being an emergent phenomenon as a result of the imperfect maintenance and repair systems. Although evolutionary processes did not select for any specific ageing genes that restrict and determine the lifespan...... of an individual, the term ‘gerontogenes’ primarily refers to any genes that may seem to influence ageing and longevity, without being specifically selected for that role. Such genes can also be called ‘virtual gerontogenes’ by virtue of their indirect influence on the rate and process of ageing. More than 1000...... virtual gerontogenes have been associated with ageing and longevity in model organisms and humans. The ‘real’ genes, which do influence the essential lifespan of a species, and have been selected for in accordance with the evolutionary life history of the species, are known as the longevity assurance...

  20. Successful Aging

    Directory of Open Access Journals (Sweden)

    Taufiqurrahman Nasihun

    2015-06-01

    Full Text Available The emerging concept of successful aging is based on evidence that in healthy individual when they get aged, there are  considerable variations in physiological functions alteration. Some people exhibiting greater, but others very few or no age related alteration. The first is called poor aging and the later is called successful pattern of aging (Lambert SW, 2008. Thus, in the simple words the successful aging concept is define as an opportunity of old people to stay  active and productive condition despite they get aged chronologically. Aging itself might be defined as the progressive accumulation of changes with time associated with or responsible for the ever-increasing susceptibility to disease and death which accompanies advancing age (Harman D, 1981. The time needed to accumulate changes is attributable to aging process. The marked emerging questions are how does aging happen and where does aging start? To answer these questions and because of the complexity of aging process, there are more than 300 aging theories have been proposed to explain how and where aging occured and started respectively. There are too many to enumerate theories and classification of aging process. In summary, all of these aging theories can be grouped into three clusters: 1. Genetics program theory, this theory suggests that aging is resulted from program directed by the genes; 2. Epigenetic theory, in these theory aging is resulted from environmental random events not determined by the genes; 3. Evolutionary theory, which propose that aging is a medium for disposal mortal soma in order to avoid competition between organism and their progeny for food and space, did not try to explain how aging occur, but possibly answer why aging occur (De la Fuente. 2009. Among the three groups of aging theories, the epigenetic theory is useful to explain and try to solve the enigma of aging which is prominently caused by internal and external environmental influences

  1. An application of safer for the Upper East Fork Poplar Creek characterization area at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Lutz, C.T.; Provost, C.A.

    1996-01-01

    The Streamlined Approach for Environmental Restoration (SAFER) has been applied at the US Department of Energy's Y-12 Plant in Oak Ridge, Tennessee. The Y-12 Plant is an operationally and hydrogeologically complex area located within the watershed of Upper East Fork Poplar Creek (UEFPC). The plant has been in operation since 1943 and nearly 175 potentially contaminated sites resulting from past waste management practices have been identified. The need to complete Remedial Investigations (RIs) for the sites in a timely and cost-effective manner has resulted in an approach that considers the entire watershed of UEFPC, which has been designated a open-quotes Characterization Areaclose quotes (CA). This approach emphasizes the watershed rather than individual sites, focuses on key questions and issues, and maximizes the use of existing data. The goal of this approach is to focus work toward the resolution of key questions and decisions necessary to complete the remediation of the CA. An evaluation of the potentially contaminated sites, the development of key questions, and the compilation and analysis of existing data are progressing. A SAFER workshop will be held in 1996, which will allow the project team and stakeholders to discuss the status of the RI, identify additional key questions and issues, and determine the activities necessary to complete the RI. This investigation demonstrates an approach to streamlining the RI process that could be applied successfully to other complex sites

  2. Remedial Investigation Work Plan for Upper East Fork Poplar Creek Operable Unit 3 at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Environmental Restoration Program

    Energy Technology Data Exchange (ETDEWEB)

    1993-08-01

    Upper East Fork Popular Creek Operable Unit 3 (UEFPC OU 3) is a source term OU composed of seven sites, and is located in the western portion of the Y-12 Plant. For the most part, the UEFPC OU 3 sites served unrelated purposes and are geographically removed from one another. The seven sites include the following: Building 81-10, the S-2 Site, Salvage Yard oil storage tanks, the Salvage Yard oil/solvent drum storage area, Tank Site 2063-U, the Salvage Yard drum deheader, and the Salvage Yard scrap metal storage area. All of these sites are contaminated with at least one or more hazardous and/or radioactive chemicals. All sites have had some previous investigation under the Y-12 Plant RCRA Program. The work plan contains summaries of geographical, historical, operational, geological, and hydrological information specific to each OU 3 site. The potential for release of contaminants to receptors through various media is addressed, and a sampling and analysis plan is presented to obtain objectives for the remedial investigation. Proposed sampling activities are contingent upon the screening level risk assessment, which includes shallow soil sampling, soil borings, monitoring well installation, groundwater sampling, and surface water sampling. Data from the site characterization activities will be used to meet the above objectives. A Field Sampling Investigation Plan, Health and Safety Plan, and Waste Management Plan are also included in this work plan.

  3. Bench- and pilot-scale demonstration of thermal desorption for removal of mercury from the Lower East Fork Poplar Creek floodplain soils

    International Nuclear Information System (INIS)

    Morris, M.I.; Sams, R.J.; Gillis, G.; Helsel, R.W.; Alperin, E.S.; Geisler, T.J.; Groen, A.; Root, D.

    1995-01-01

    Thermal desorption is an innovative technology that has seen significant growth in applications to organically contaminated soils and sludges for the remediation of hazardous, radioactive and mixed waste sites. This paper will present the results of a bench and pilot-scale demonstration of this technology for the removal of mercury from the Lower East Fork Poplar Creek floodplain soil. Results demonstrate that the mercury in this soil can be successfully removed to the target treatment levels of 10 milligrams per kilogram (mg/kg) and that all process residuals could be rendered RCRA-nonhazardous as defined by the Resource Conservation and Recovery Act. Sampling and analyses of the desorber off-gas before and after the air pollution control system demonstrated effective collection of mercury and organic constituents. Pilot-scale testing was also conducted to verify requirements for material handling of soil into and out of the process. This paper will also present a conceptual design and preliminary costs of a full-scale system, including feed preparation, thermal treatment, and residuals handling for the soil

  4. Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007

    Science.gov (United States)

    Alvarez, David A.; Cranor, Walter L.; Perkins, Stephanie D.; Schroeder, Vickie L.; Werner, Stephen; Furlong, Edward T.; Holmes, John

    2008-01-01

    Declining fish health, fish exhibiting external lesions, incidences of intersex, and death, have been observed recently within the Potomac River basin. The basin receives surface runoff and direct inputs from agricultural, industrial, and other human activities. Two locations on the North Fork of the Shenandoah River were selected for study in an attempt to identify chemicals that may have contributed to the declining fish health. Two passive sampling devices, semipermeable membrane devices (SPMDs) and polar organic chemical integrative samplers (POCIS), were deployed during consecutive two-month periods during the spring and early summer of 2007 to measure select organic contaminants to which fish may have been exposed. This study determined that concentrations of persistent hydrophobic contaminants, such as polycyclic aromatic hydrocarbons (wastewater treatment plant effluent or septic tank discharges were identified. In contrast, para-cresol, N,N-diethyltoluamide, and caffeine commonly were detected. Prescription pharmaceuticals including carbamazepine, venlafaxine, and 17a-ethynylestradiol were at low concentrations. Extracts from the passive samplers also were screened for the presence of estrogenic chemicals using the yeast estrogen screen. An estrogenic response was observed in POCIS samples from both sites, whereas SPMD samples exhibited little to no estrogenicity. This indicates that the chemicals producing the estrogenic response have a greater water solubility and are, therefore, less likely to bioaccumulate in fatty tissues of organisms.

  5. An aerial radiological survey of the Oyster Creek Nuclear Power Plant and surrounding area, Forked River, New Jersey. Date of survey: September 18--25, 1992

    International Nuclear Information System (INIS)

    Hopkins, H.A.; McCall, K.A.

    1994-05-01

    An aerial radiological survey was conducted over the Oyster Creek Nuclear Power Plant in Forked River, New Jersey, during the period September 18 through September 24, 1992. The survey was conducted at an altitude of 150 feet (46 meters) over a 26-square-mile (67-square-kilometer) area centered on the power station. The purpose of the survey was to document the terrestrial gamma radiation environment of the Oyster Creek Nuclear Power plant and surrounding area. The results of the aerial survey are reported as inferred gamma radiation exposure rates at 1 meter above ground level in the form of a contour map. Outside the plant boundary, exposure rates were found to vary between 4 and 10 microroentgens per hour and were attributed to naturally-occurring uranium, thorium, and radioactive potassium gamma emitters. The aerial data were compared to ground-based benchmark exposure rate measurements and radionuclide assays of soil samples obtained within the survey boundary. The ground-based measurements were found to be in good agreement with those inferred from the aerial measuring system. A previous survey of the power plant was conducted in August 1969 during its initial startup phase. Exposure rates and radioactive isotopes revealed in both surveys were consistent and within normal terrestrial background levels

  6. A physiological approach to quantifying thermal habitat quality for redband rainbow trout (Oncorhynchus mykiss gairdneri) in the south Fork John Day River, Oregon

    Science.gov (United States)

    Feldhaus, J.W.; Heppell, S.A.; Li, H.; Mesa, M.G.

    2010-01-01

    We examined tissue-specific levels of heat shock protein 70 (hsp70) and whole body lipid levels in juvenile redband trout (Oncorhynchus mykiss gairdneri) from the South Fork of the John Day River (SFJD), Oregon, with the goal of determining if these measures could be used as physiological indicators of thermal habitat quality for juvenile redband trout. Our objectives were to determine the hsp70 induction temperature in liver, fin, and white muscle tissue and characterize the relation between whole body lipids and hsp70 for fish in the SFJD. We found significant increases in hsp70 levels between 19 and 22??C in fin, liver, and white muscle tissue. Maximum hsp70 levels in liver, fin, and white muscle tissue occurred when mean weekly maximum temperatures (MWMT) exceeded 20-22??C. In general, the estimated hsp70 induction temperature for fin and white muscle tissue was higher than liver tissue. Whole body lipid levels began to decrease when MWMT exceeded 20. 4??C. There was a significant interaction between temperature and hsp70 in fin and white muscle tissue, but not liver tissue. Collectively, these results suggest that increased hsp70 levels in juvenile redband trout are symptomatic of thermal stress, and that energy storage capacity decreases with this stress. The possible decrease in growth potential and fitness for thermally stressed individuals emphasizes the physiological justification for thermal management criteria in salmon-bearing streams. ?? Springer Science+Business Media B.V. 2010.

  7. Quartz enhanced photoacoustic H{sub 2}S gas sensor based on a fiber-amplifier source and a custom tuning fork with large prong spacing

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Hongpeng; Liu, Xiaoli; Zheng, Huadan; Yin, Xukun; Ma, Weiguang; Zhang, Lei; Yin, Wangbao; Jia, Suotang [State Key Laboratory of Quantum Optics and Quantum Optics Devices, Institute of Laser Spectroscopy, Shanxi University, Taiyuan 030006 (China); Sampaolo, Angelo [Dipartimento Interateneo di Fisica, Università degli Studi di Bari and Politecnico di Bari, CNR-IFN UOS BARI, Via Amendola 173, Bari 70126 (Italy); Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005 (United States); Dong, Lei, E-mail: donglei@sxu.edu.cn [State Key Laboratory of Quantum Optics and Quantum Optics Devices, Institute of Laser Spectroscopy, Shanxi University, Taiyuan 030006 (China); Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005 (United States); Patimisco, Pietro; Spagnolo, Vincenzo [Dipartimento Interateneo di Fisica, Università degli Studi di Bari and Politecnico di Bari, CNR-IFN UOS BARI, Via Amendola 173, Bari 70126 (Italy); Tittel, Frank K. [Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005 (United States)

    2015-09-14

    A quartz enhanced photoacoustic spectroscopy (QEPAS) sensor, employing an erbium-doped fiber amplified laser source and a custom quartz tuning fork (QTF) with its two prongs spaced ∼800 μm apart, is reported. The sensor employs an acoustic micro-resonator (AmR) which is assembled in an “on-beam” QEPAS configuration. Both length and vertical position of the AmR are optimized in terms of signal-to-noise ratio, significantly improving the QEPAS detection sensitivity by a factor of ∼40, compared to the case of a sensor using a bare custom QTF. The fiber-amplifier-enhanced QEPAS sensor is applied to H{sub 2}S trace gas detection, reaching a sensitivity of ∼890 ppb at 1 s integration time, similar to those obtained with a power-enhanced QEPAS sensor equipped with a standard QTF, but with the advantages of easy optical alignment, simple installation, and long-term stability.

  8. A probabilistic risk assessment of the effects of methylmercury and PCBs on mink and kingfishers along East Fork Poplar Creek, Oak Ridge, Tennessee, USA

    Energy Technology Data Exchange (ETDEWEB)

    Moore, D.R.J.; Sample, B.E.; Suter, G.W.; Parkhurst, B.R.; Teed, R.S.

    1999-12-01

    Over fifty years of operations, storage, and disposal of wastes from the US Department of Energy (US DOE) Y-12 nuclear weapons facility at Oak Ridge, Tennessee, USA, has resulted in the contamination of water, sediment, biota, and floodplain soils of East Fork Poplar Creek. A preliminary assessment revealed that methylmercury and polychlorinated biphenyls (PCBs) were the contaminants of most concern. Because these contaminants are persistent, accumulate in tissues, and biomagnify up the food chain, piscivorous wildlife are the biota at greatest risk of exposure. The objective of this study was to estimate the risks posed by methylmercury and PCBs to two piscivorous species: mink and belted kingfishers. The authors conducted Monte Carlo simulations to estimate total daily intakes of each contaminant by each species and then integrated the resulting distributions with their respective dose-response curves to estimate risks. The results indicate that methylmercury poses a moderate risk to female mink (24% probability of at least 15% mortality) and kingfishers (50% probability of at least a 12--28% decline in fecundity depending on location). The PCBs pose a very serious risk to mink (52% probability of at least a 50% decline in reproductive fecundity), a species known to be especially sensitive to the effects of organochlorine substances, but little risk to kingfishers (<5% probability of a decline in reproductive fecundity greater than 10% at any location).

  9. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant: Data interpretations and proposed program modifications

    International Nuclear Information System (INIS)

    1991-06-01

    This report is a detailed assessment of groundwater quality at several hazardous waste-management facilities associated with the Department of Energy (DOE) Y-12 Plant in Oak Ridge, Tennessee. The sites are located in an area defined as the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three hydrogeologic regimes that have been established at the Y-12 Plant in an effort to unify and coordinate site-specific monitoring activities for planning and reporting purposes. Section 2.0 contains background information and a discussion of the 1990 program objectives. An overview of the complex hydrogeologic system in the UEFPCHR is provided in Section 3.0. A discussion of the interpretive assumptions used in evaluating the 1990 assessment data, a review of groundwater geochemistry and background water quality, detailed descriptions of groundwater contaminant plumes, and a discussion regarding the quality of groundwater exiting the UEFPCHR are presented in Section 4.0. Findings of the 1990 assessment program are summarized in Section 5.0. Modifications to the assessment monitoring program proposed for 1991 are presented in Section 6.0, and a list of references (Section 7.0) concludes the report. 20 refs., 23 figs., 10 tabs

  10. A self-strain feedback tuning-fork-shaped ionic polymer metal composite clamping actuator with soft matter elasticity-detecting capability for biomedical applications.

    Science.gov (United States)

    Feng, Guo-Hua; Huang, Wei-Lun

    2014-12-01

    This paper presents a smart tuning-fork-shaped ionic polymer metal composite (IPMC) clamping actuator for biomedical applications. The two fingers of the actuator, which perform the clamping motion, can be electrically controlled through a unique electrode design on the IPMC material. The generated displacement or strain of the fingers can be sensed using an integrated soft strain-gage sensor. The IPMC actuator and associated soft strain gage were fabricated using a micromachining technique. A 13.5×4×2 mm(3) actuator was shaped from Nafion solution and a selectively grown metal electrode formed the active region. The strain gage consisted of patterned copper foil and polyethylene as a substrate. The relationship between the strain gage voltage output and the displacement at the front end of the actuator's fingers was characterized. The equivalent Young's modulus, 13.65 MPa, of the soft-strain-gage-integrated IPMC finger was analyzed. The produced clamping force exhibited a linear increasing rate of 1.07 mN/s, based on a dc driving voltage of 7 V. Using the developed actuator to clamp soft matter and simultaneously acquire its Young's modulus was achieved. This demonstrated the feasibility of the palpation function and the potential use of the actuator in minimally invasive surgery. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Investigation on the Mechanical and Electrical Behavior of a Tuning Fork-Shaped Ionic Polymer Metal Composite Actuator with a Continuous Water Supply Mechanism.

    Science.gov (United States)

    Feng, Guo-Hua; Huang, Wei-Lun

    2016-03-25

    This paper presents an innovative tuning fork-shaped ionic polymer metal composite (IPMC) actuator. With an integrated soft strain gauge and water supply mechanism (WSM), the surface strain of the actuator can be sensed in situ, and providing a continuous water supply maintains the water content inside the IPMC for long-term operation in air. The actuator was fabricated using a micromachining technique and plated with a nickel electrode. The device performance was experimentally characterized and compared with an actuator without a WSM. A large displacement of 1.5 mm was achieved for a 6 mm-long prong with 7-V dc actuation applied for 30 s. The measured current was analyzed using an electrochemical model. The results revealed that the faradaic current plays a crucial role during operation, particularly after 10 s. The measured strain confirms both the bending and axial strain generation during the open-and-close motion of the actuator prongs. Most of the water loss during device operation was due to evaporation rather than hydrolysis. The constructed WSM effectively maintained the water content inside the IPMC for long-term continuous operation.

  12. Remedial Investigation Work Plan for Upper East Fork Poplar Creek Operable Unit 3 at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1993-08-01

    Upper East Fork Popular Creek Operable Unit 3 (UEFPC OU 3) is a source term OU composed of seven sites, and is located in the western portion of the Y-12 Plant. For the most part, the UEFPC OU 3 sites served unrelated purposes and are geographically removed from one another. The seven sites include the following: Building 81-10, the S-2 Site, Salvage Yard oil storage tanks, the Salvage Yard oil/solvent drum storage area, Tank Site 2063-U, the Salvage Yard drum deheader, and the Salvage Yard scrap metal storage area. All of these sites are contaminated with at least one or more hazardous and/or radioactive chemicals. All sites have had some previous investigation under the Y-12 Plant RCRA Program. The work plan contains summaries of geographical, historical, operational, geological, and hydrological information specific to each OU 3 site. The potential for release of contaminants to receptors through various media is addressed, and a sampling and analysis plan is presented to obtain objectives for the remedial investigation. Proposed sampling activities are contingent upon the screening level risk assessment, which includes shallow soil sampling, soil borings, monitoring well installation, groundwater sampling, and surface water sampling. Data from the site characterization activities will be used to meet the above objectives. A Field Sampling Investigation Plan, Health and Safety Plan, and Waste Management Plan are also included in this work plan

  13. Reproductive Consequences of Nest Site Use in Fork-tailed Storm-Petrels in the Aleutian Islands, Alaska: Potential Lasting Effects of an Introduced Predator

    Directory of Open Access Journals (Sweden)

    Brie A. Drummond

    2010-12-01

    Full Text Available We examined the reproductive consequences of differential nest site use in Fork-tailed Storm-Petrels (Oceanodroma furcata in the Aleutian Islands, Alaska, where birds on islands where foxes were introduced nest in rocky substrate rather than in typical soil habitat. We investigated how physical and microclimatic nest site characteristics influenced storm-petrel breeding success 20 years after fox removal. We then examined whether those nest site characteristics that affected success were related to the amount of rock that composed the nest. In both years of our study, nest temperature had the strongest influence on chick survival and overall reproductive success, appearing in all the top models and alone explaining 14-35% of the variation in chick survival. The relationship between reproductive success and nest temperature was positive in both years, with higher survival in warmer nests. In turn, the best predictor of nest temperature was the amount of rock that composed the site. Rockier nests had colder average temperatures, which were driven by lower daily minimum temperatures, compared to nests with more soil. Thus, the rockiness of the nest site appeared to affect chick survival and overall reproductive success through its influence on nest temperature. This study suggests that the use of rocky nest sites, presumed to be a result of historic predation from introduced foxes, could decrease breeding success in this recovering population, and thus be a long-lasting effect of introduced predators.

  14. Groundwater quality assessment for the Upper East Fork Poplar Creek Hydrogeologic Regime at the Y-12 Plant. 1991 groundwater quality data and calculated rate of contaminant migration

    Energy Technology Data Exchange (ETDEWEB)

    1992-02-01

    This report contains groundwater quality data obtained during the 1991 calendar year at several waste management facilities and petroleum fuel underground storage tank (UST) sites associated with the Y-12 Plant. These sites are within the Upper East Fork Poplar Creek Hydrogeologic Regime (UEFPCHR), which is one of three regimes defined for the purposes of groundwater and surface-water quality monitoring and remediation. This report was prepared for informational purposes. Included are the analytical data for groundwater samples collected from selected monitoring wells during 1991 and the results for quality assurance/quality control (QA/QC) samples associated with each groundwater sample. This report also contains summaries of selected data, including ion-charge balances for each groundwater sample, a summary of analytical results for nitrate (a principle contaminant in the UEFPCHR), results of volatile organic compounds (VOCs) analyses validated using the associated QA/QC sample data, a summary of trace metal concentrations which exceeded drinking-water standards, and a summary of radiochemical analyses and associated counting errors.

  15. Fork stalling and template switching as a mechanism for polyalanine tract expansion affecting the DYC mutant of HOXD13, a new murine model of synpolydactyly.

    Science.gov (United States)

    Cocquempot, Olivier; Brault, Véronique; Babinet, Charles; Herault, Yann

    2009-09-01

    Polyalanine expansion diseases are proposed to result from unequal crossover of sister chromatids that increases the number of repeats. In this report we suggest an alternative mechanism we put forward while we investigated a new spontaneous mutant that we named "Dyc" for "Digit in Y and Carpe" phenotype. Phenotypic analysis revealed an abnormal limb patterning similar to that of the human inherited congenital disease synpolydactyly (SPD) and to the mouse mutant model Spdh. Both human SPD and mouse Spdh mutations affect the Hoxd13 gene within a 15-residue polyalanine-encoding repeat in the first exon of the gene, leading to a dominant negative HOXD13. Genetic analysis of the Dyc mutant revealed a trinucleotide expansion in the polyalanine-encoding region of the Hoxd13 gene resulting in a 7-alanine expansion. However, unlike the Spdh mutation, this expansion cannot result from a simple duplication of a short segment. Instead, we propose the fork stalling and template switching (FosTeS) described for generation of nonrecurrent genomic rearrangements as a possible mechanism for the Dyc polyalanine extension, as well as for other polyalanine expansions described in the literature and that could not be explained by unequal crossing over.

  16. Age Spots

    Science.gov (United States)

    ... for Every Season How to Choose the Best Skin Care Products In This Section Dermatologic Surgery What is dermatologic ... for Every Season How to Choose the Best Skin Care Products Age Spots Treatment Options Learn more about treatment ...

  17. Premature aging

    International Nuclear Information System (INIS)

    Sassaki, Hideo

    1992-01-01

    The hypothesis that radiation may accelerate aging phenomenon has been studied extensively, using the population of A-bomb survivors. In this paper, non-specific radiation-induced premature aging is discussed with a review of the literature. Cardiac lipofuscin, papillary fibrosis, aortic extensibility, hexamine/collagen ratio in the skin and aorta, testicular changes, giant hepatic cell nucleus, and neurofibril changes have so far been studied pathologically in the context of A-bomb radiation. Only testicular sclerosis has been found to correlate with distance from the hypocenter. Suggestive correlation was found to exist between the hexamine/collagen ratio in the skin and aorta and A-bomb radiation. Grip strength and hearing ability were decreased in the group of 100 rad and the group of 50-99 rad, respectively. The other physiological data did not definitely correlate with A-bomb radiation. Laboratory data, including erythrocyte sedimentation rate, α and β globulin levels, phytohemagglutinin reaction, T cell counts, erythrocyte glycophorin-A, the incidence of cerebral stroke, ischemic heart disease, and cataract were age-dependent and correlated with A-bomb radiation. These findings indicated that the occurrence of arteriosclerosis-related diseases, changes in immunological competence, and some pathological and physiological findings altered with advancing age, suggesting the correlation with A-bomb radiation. In general, it cannot be concluded that there is a positive correlation between A-bomb radiation and the premature aging. (N.K.) 51 refs

  18. DNA secondary structure of the released strand stimulates WRN helicase action on forked duplexes without coordinate action of WRN exonuclease

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Byungchan, E-mail: bbccahn@mail.ulsan.ac.kr [Department of Life Sciences, University of Ulsan, Ulsan (Korea, Republic of); Bohr, Vilhelm A. [Laboratory of Molecular Gerontology, Biomedical Research Center, National Institute on Aging, Baltimore, MD (United States)

    2011-08-12

    Highlights: {yields} In this study, we investigated the effect of a DNA secondary structure on the two WRN activities. {yields} We found that a DNA secondary structure of the displaced strand during unwinding stimulates WRN helicase without coordinate action of WRN exonuclease. {yields} These results imply that WRN helicase and exonuclease activities can act independently. -- Abstract: Werner syndrome (WS) is an autosomal recessive premature aging disorder characterized by aging-related phenotypes and genomic instability. WS is caused by mutations in a gene encoding a nuclear protein, Werner syndrome protein (WRN), a member of the RecQ helicase family, that interestingly possesses both helicase and exonuclease activities. Previous studies have shown that the two activities act in concert on a single substrate. We investigated the effect of a DNA secondary structure on the two WRN activities and found that a DNA secondary structure of the displaced strand during unwinding stimulates WRN helicase without coordinate action of WRN exonuclease. These results imply that WRN helicase and exonuclease activities can act independently, and we propose that the uncoordinated action may be relevant to the in vivo activity of WRN.

  19. Successful ageing

    DEFF Research Database (Denmark)

    Bülow, Morten Hillgaard; Söderqvist, Thomas

    2014-01-01

    Since the late 1980s, the concept of ‘ successful ageing’ has set the frame for discourse about contemporary ageing research. Through an analysis of the reception to John W. Rowe and Robert L. Kahn's launch of the concept of ‘ successful ageing’ in 1987, this article maps out the important themes...... and discussions that have emerged from the interdisciplinary field of ageing research. These include an emphasis on interdisciplinarity; the interaction between biology, psycho-social contexts and lifestyle choices; the experiences of elderly people; life-course perspectives; optimisation and prevention...... strategies; and the importance of individual, societal and scientific conceptualisations and understandings of ageing. By presenting an account of the recent historical uses, interpretations and critiques of the concept, the article unfolds the practical and normative complexities of ‘ successful ageing’....

  20. Aging Differently

    DEFF Research Database (Denmark)

    Zajitschek, Felix; Jin, Tuo; Colchero, Fernando

    2014-01-01

    Diet effects on age-dependent mortality patterns are well documented in a large number of animal species, but studies that look at the effects of nutrient availability on late-life mortality plateaus are lacking. Here, we focus on the effect of dietary protein content (low, intermediate, and high...... based on Bayesian inference of age-specific mortality rates and found a deceleration of late-life mortality rates on all diets in males but only on the intermediate (standard) diet in females. The difference in mortality rate deceleration between males and females on extreme diets suggests...

  1. Aging Well

    DEFF Research Database (Denmark)

    Frieman, Catherine J.; Bruck, Joanna; Rebay-Salisbury, Katharina

    2017-01-01

    This article consist of several short individual articles which were solicited as responses to and reflections on Paul Treherne’s seminal meditation on Bronze Age male identity and warriorhood. Authors were asked to build on Treherne’s work and to reflect on how it had impacted their own research...

  2. Ice Ages

    Indian Academy of Sciences (India)

    that the precession of the earth's orbit caused ice ages. The precession of the earth's orbit leads to changes in the time of the year at which ... than in the southern hemisphere. ..... small increase in ocean temperature implies a large increase in.

  3. A high performance micro-pressure sensor based on a double-ended quartz tuning fork and silicon diaphragm in atmospheric packaging

    International Nuclear Information System (INIS)

    Cheng, Rongjun; Li, Cun; Zhao, Yulong; Li, Bo; Tian, Bian

    2015-01-01

    A resonant micro-pressure sensor based on a double-ended quartz tuning fork (DEQTF) and bossed silicon diaphragm in atmospheric packaging is presented. To achieve vacuum-free packaging with a high quality factor, the DEQTF is designed to resonate in an anti-phase vibration mode in a plane that is under the effect of slide-film damping. The feasibility is demonstrated by theoretical analysis and a finite element simulation. The dimensions of the DEQTF and diaphragm are optimized in accordance with the principles of improving sensitivity and minimizing energy dissipation. The sensor chip is fabricated using quartz and silicon micromachining technologies, and simply packaged in a stainless steel shell with standard atmosphere. The experimental setup is established for the calibration, where an additional sensor prototype without a pressure port is introduced as a frequency reference. By detecting the frequency difference of the tested sensor and reference sensor, the influences of environmental factors such as temperature and shocks on measuring accuracy are eliminated effectively. Under the action of a self-excitation circuit, static performance is obtained. The sensitivity of the sensor is 299 kHz kPa −1 in the operating range of 0–10 kPa at room temperature. Testing results shows a nonlinearity of 0.0278%FS, a hysteresis of 0.0207%FS and a repeatability of 0.0375%FS. The results indicate that the proposed sensor has favorable features, which provides a cost-effective and high-performance approach for low pressure measurement. (paper)

  4. Proposed experiment for SnCl2 treatment of Outfall 200 for the purpose of mercury removal from East Fork Poplar Creek, Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    Southworth, G.R.

    1997-03-01

    Identification and treatment/elimination of point sources of mercury (Hg) to East Fork Poplar Creek (EFPC) within the Y-12 Plant have reduced base flow mercury concentrations considerably; but, after all such actions are completed, nonpoint sources will continue to add mercury to the creek. Studies conducted in 1996 on the use of air stripping to remove elemental mercury from Outfall 51, a mercury-contaminated natural spring, demonstrated that the addition of trace concentrations of stannous chloride (SnCl 2 ) converted a large fraction of the dissolved mercury in the outfall to elemental mercury, which could subsequently be removed by air stripping. Dissolved mercury is the dominant form in EFPC at the north/south (N/S) pipes, where it emerges from the underground storm drain network. More than 50% of that mercury is capable of being rapidly reduced by the addition of a 3--5 fold molar excess of stannous chloride. Upon conversion to the volatile gaseous (elemental) form, mercury would be lost across the air-water interface through natural volatilization. EFPC within the Y-12 Plant is shallow, turbulent, and open to sunlight and wind, providing conditions that facilitate natural evasion of volatile chemicals from the water. Preliminary calculations estimate that 75% or more of the elemental mercury could be removed via evasion between the N/S pipes and the Y-l2 Plant boundary (Station 17). Alternatively, elemental mercury might be removed from EFPC in a short reach of stream below the N/S pipes by an in-situ air stripping system which bubbles air through the water column. The purpose of these proposed experiments is to test whether natural volatilization or in-situ air stripping may be used to further reduce baseflow concentrations of mercury in EFPC. Results of this experiment will be useful for understanding the transport and fate of other volatile chemicals in the upper reaches of EFPC

  5. Hyporheic Exchange Flows and Biogeochemical Patterns near a Meandering Stream: East Fork of the Jemez River, Valles Caldera National Preserve, New Mexico

    Science.gov (United States)

    Christensen, H.; Wooten, J. P.; Swanson, E.; Senison, J. J.; Myers, K. D.; Befus, K. M.; Warden, J.; Zamora, P. B.; Gomez, J. D.; Wilson, J. L.; Groffman, A.; Rearick, M. S.; Cardenas, M. B.

    2012-12-01

    A study by the 2012 Hydrogeology Field Methods class of the University of Texas at Austin implemented multiple approaches to evaluate and characterize local hyporheic zone flow and biogeochemical trends in a highly meandering reach of the of the East Fork of the Jemez River, a fourth order stream in northwestern New Mexico. This section of the Jemez River is strongly meandering and exhibits distinct riffle-pool morphology. The high stream sinuosity creates inter-meander hyporheic flow that is also largely influenced by local groundwater gradients. In this study, dozens of piezometers were used to map the water table and flow vectors were then calculated. Surface water and ground water samples were collected and preserved for later geochemical analysis by ICPMS and HPLC, and unstable parameters and alkalinity were measured on-site. Additionally, information was collected from thermal monitoring of the streambed, stream gauging, and from a series of electrical resistivity surveys forming a network across the site. Hyporheic flow paths are suggested by alternating gaining and losing sections of the stream as determined by stream gauging at multiple locations along the reach. Water table maps and calculated fluxes across the sediment-water interface also indicate hyporheic flow paths. We find variability in the distribution of biogeochemical constituents (oxidation-reduction potential, nitrate, ammonium, and phosphate) along interpreted flow paths which is partly consistent with hyporheic exchange. The variability and heterogeneity of reducing and oxidizing conditions is interpreted to be a result of groundwater-surface water interaction. Two-dimensional mapping of biogeochemical parameters show redox transitions along interpreted flow paths. Further analysis of various measured unstable chemical parameters results in observable trends strongly delineated along these preferential flow paths that are consistent with the direction of groundwater flow and the assumed

  6. Site characterization summary report for dry weather surface water sampling upper East Fork Poplar Creek characterization area Oak Ridge Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-01

    This report describes activities associated with conducting dry weather surface water sampling of Upper East Fork Poplar Creek (UEFPC) at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. This activity is a portion of the work to be performed at UEFPC Operable Unit (OU) 1 [now known as the UEFPC Characterization Area (CA)], as described in the RCRA Facility Investigation Plan for Group 4 at the Oak- Ridge Y-12 Plant, Oak Ridge, Tennessee and in the Response to Comments and Recommendations on RCRA Facility Investigation Plan for Group 4 at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee, Volume 1, Operable Unit 1. Because these documents contained sensitive information, they were labeled as unclassified controlled nuclear information and as such are not readily available for public review. To address this issue the U.S. Department of Energy (DOE) published an unclassified, nonsensitive version of the initial plan, text and appendixes, of this Resource Conservation and Recovery Act (RCRA) Facility Investigation (RFI) Plan in early 1994. These documents describe a program for collecting four rounds of wet weather and dry weather surface water samples and one round of sediment samples from UEFPC. They provide the strategy for the overall sample collection program including dry weather sampling, wet weather sampling, and sediment sampling. Figure 1.1 is a schematic flowchart of the overall sampling strategy and other associated activities. A Quality Assurance Project Plan (QAPJP) was prepared to specifically address four rounds of dry weather surface water sampling and one round of sediment sampling. For a variety of reasons, sediment sampling has not been conducted and has been deferred to the UEFPC CA Remedial Investigation (RI), as has wet weather sampling.

  7. The Long-Term Effects of Large Wood Placement on Salmonid Habitat in East Fork Mill Creek, Redwood National and State Park, California

    Science.gov (United States)

    Rodriguez, D. L.; Stubblefield, A. P.

    2017-12-01

    The conservation and recovery of anadromous salmonids (Oncorhynchus sp.) depend on stream restoration and protection of freshwater habitats. Instream large wood dictates channel morphology, increase retention of terrestrial inputs such as organic matter, nutrients and sediment, and enhances the quality of fish habitat. Historic land use/land cover changes have resulted in aquatic systems devoid of this component. Restoration by placement of large wood jams is intended to restore physical and biological processes. An important question for scientists and managers, in addition to the initial effectiveness of restoration, is the persistence and fate of this type of project. In this study we compare channel change and large wood attributes on the East Fork of Mill Creek, a tributary of the Smith River in northern California, eight years after a major instream wood placement effort took place. Our results are compared with previously published data from before and one year after the restoration. Preliminary results suggest the dramatic increase in spawning gravel abundance and large wood accumulation observed in the earlier study have persisted. From 2008 to 2016 a reduction in median sediment size, ranging from 103-136 percent, has been observed in a majority of the sites. The sites have continued to grow in size and influence by racking floating wood from upstream and destabilizing proximate banks of riparian alder, increasing both instream large wood volume (5-196 %) and floodplain connectivity. Preliminary results also show a decrease in residual pool depth and an increase in pool length which may be attributed to floodplain connectivity. Changes to the following attributes are evaluated: 1) wood loading (total site wood volume, total wood volume in active channel, and wood piece count); 2) percent pool cover by large wood; 3) residual pool depth; 4) upstream sediment aggradation; 5) floodplain connectivity; and 6) mean sediment size directly above and below large

  8. Trophic feasibility of reintroducing anadromous salmonids in three reservoirs on the north fork Lewis River, Washington: Prey supply and consumption demand of resident fishes

    Science.gov (United States)

    Sorel, Mark H.; Hansen, Adam G.; Connelly, Kristin A.; Beauchamp, David A.

    2016-01-01

    The reintroduction of anadromous salmonids in reservoirs is being proposed with increasing frequency, requiring baseline studies to evaluate feasibility and estimate the capacity of reservoir food webs to support reintroduced populations. Using three reservoirs on the north fork Lewis River as a case study, we demonstrate a method to determine juvenile salmonid smolt rearing capacities for lakes and reservoirs. To determine if the Lewis River reservoirs can support reintroduced populations of juvenile stream-type Chinook Salmon Oncorhynchus tshawytscha, we evaluated the monthly production of daphniaDaphnia spp. (the primary zooplankton consumed by resident salmonids in the system) and used bioenergetics to model the consumption demand of resident fishes in each reservoir. To estimate the surplus of Daphnia prey available for reintroduced salmonids, we assumed a maximum sustainable exploitation rate and accounted for the consumption demand of resident fishes. The number of smolts that could have been supported was estimated by dividing any surplus Daphnia production by the simulated consumption demand of an individual Chinook Salmon fry rearing in the reservoir to successful smolt size. In all three reservoirs, densities of Daphnia were highest in the epilimnion, but warm epilimnetic temperatures and the vertical distribution of planktivores suggested that access to abundant epilimnetic prey was limited. By comparing accessible prey supply and demand on a monthly basis, we were able to identify potential prey supply bottlenecks that could limit smolt production and growth. These results demonstrate that a bioenergetics approach can be a valuable method of examining constraints on lake and reservoir rearing capacity, such as thermal structure and temporal food supply. This method enables numerical estimation of rearing capacity, which is a useful metric for managers evaluating the feasibility of reintroducing Pacific salmon Oncorhynchus spp. in lentic systems.

  9. Quantifying the net benefit impacts of the Troy Waste Water Treatment Plant on Steelhead Habitat in the West Fork Little Bear Creek drainage

    Science.gov (United States)

    Sanchez-Murillo, R.; Brooks, E. S.; Boll, J.

    2010-12-01

    Discharge of waste water treatment plants (WWTPs) typically is viewed to result in water quality impairment. However, WWTPs can also be a source of nutrients to enhance the salmonid food web as well as an efficient way to maintain acceptable water temperature regimes and flow conditions during summer. We observed this paradox in West Fork Little Bear Creek (WFLB) in the City of Troy, Idaho. Despite the nutrient load, the WFLB had the highest Steelhead trout density in the watershed, with a mean density of 13.2 fish/100 m2. The objective of this project was to utilize a water quality model, QUAL2kw, and an ecology assessment to examine how the nutrient load from the WWTP affects: a) habitat conditions for steelhead juveniles, and b) physic-chemical parameters. Four monitoring stations were installed from May through November in 2009 and 2010. An undisturbed creek was used as a control site in 2010. Dissolved oxygen (DO), electrical conductivity, temperature, and discharge were measured continuously at each monitoring station. Weekly samples were collected at each monitoring station and analyzed for nitrate, nitrite, ammonia, total Kjeldahl nitrogen, total phosphorous, and orthophosphates. In 2010, Chlorophyll a was analyzed weekly, while bottom algae biomass was determined monthly. Results show that during summer months, the WWTP provides the majority of the flow (0.1 cfs) in the creek. Water samples and DO measurements taken 200 m downstream of the plant during late summer months indicate that nitrification process leads to low DO level well below the state standard of 6 mg/L for cold water biota. However dissolved oxygen levels recover within 1 km downstream. Discharge data suggest that without the flow from the treatment most of the creek would dry during late summer months. Abundance of macroinverbrates, high primary productivity, and sustained flow during summer suggests that the effluent from the WWTP is a net benefit to the Steelhead habitat in the basin

  10. Calendar year 1993 groundwater quality report for the Upper East Fork Poplar Creek hydrogeologic regime Y-12 Plant, Oak Ridge, Tennessee: 1993 groundwater quality data interpretations and proposed program modifications

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-10-01

    This Groundwater Quality Report (GWQR) contains an evaluation of the groundwater quality data obtained during the 1993 calendar year (CY) at the U.S. Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee (Figure 1). The groundwater quality data are presented in Part 1 of the GWQR submitted by Martin Marietta Energy Systems, Inc. (Energy Systems) to the Tennessee Department of Environment and Conservation (TDEC) in February 1994 (HSW Environmental Consultants, Inc. 1994a). Groundwater quality data evaluated in this report were obtained at several hazardous and non-hazardous waste management facilities and underground storage tanks (USTS) located within the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability Organization manages the groundwater monitoring activities in each regime under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). The purpose of the GWPP is to characterize the hydrogeology and to monitor groundwater quality at the Y-12 Plant and surrounding area to provide for protection of groundwater resources consistent with federal, state, and local requirements and in accordance with DOE Orders and Energy Systems corporate policy. The annual GWQR for the East Fork Regime is completed in two parts. Part 1 consists primarily of data appendices and serves as a reference for the groundwater quality data obtained each CY under the lead of the Y-12 Plant GWPP. Part 2 (this report) contains an evaluation of the data with respect to regime-wide groundwater quality, presents the findings and status of ongoing hydrogeologic studies, describes changes in monitoring priorities, and presents planned modifications to the groundwater sampling and analysis program for the following calendar year.

  11. Changes in streamflow and summary of major-ion chemistry and loads in the North Fork Red River basin upstream from Lake Altus, northwestern Texas and western Oklahoma, 1945-1999

    Science.gov (United States)

    Smith, S. Jerrod; Wahl, Kenneth L.

    2003-01-01

    Upstream from Lake Altus, the North Fork Red River drains an area of 2,515 square miles. The quantity and quality of surface water are major concerns at Lake Altus, and water-resource managers and consumers need historical information to make informed decisions about future development. The Lugert-Altus Irrigation District relies on withdrawals from the lake to sustain nearly 46,000 acres of agricultural land. Kendall's tau tests of precipitation data indicated no statistically significant trend over the entire 100 years of available record. However, a significant increase in precipitation occurred in the last 51 years. Four streamflow-gaging stations with more than 10 years of record were maintained in the basin. These stations recorded no significant trends in annual streamflow volume. Two stations, however, had significant increasing trends in the base-flow index, and three had significant decreasing trends in annual peak flows. Major-ion chemistry in the North Fork Red River is closely related to the chemical composition of the underlying bedrock. Two main lithologies are represented in the basin upstream from Lake Altus. In the upper reaches, young and poorly consolidated sediments include a range of sizes from coarse gravel to silt and clay. Nearsurface horizons commonly are cemented as calcium carbonate caliche. Finer-grained gypsiferous sandstones and shales dominate the lower reaches of the basin. A distinct increase in dissolved solids, specifically sodium, chloride, calcium, and sulfate, occurs as the river flows over rocks that contain substantial quantities of gypsum, anhydrite, and dolomite. These natural salts are the major dissolved constituents in the North Fork Red River.

  12. Calendar year 1993 groundwater quality report for the Upper East Fork Poplar Creek hydrogeologic regime Y-12 Plant, Oak Ridge, Tennessee: 1993 groundwater quality data interpretations and proposed program modifications

    International Nuclear Information System (INIS)

    1994-10-01

    This Groundwater Quality Report (GWQR) contains an evaluation of the groundwater quality data obtained during the 1993 calendar year (CY) at the U.S. Department of Energy (DOE) Y-12 Plant located on the DOE Oak Ridge Reservation (ORR) southeast of Oak Ridge, Tennessee (Figure 1). The groundwater quality data are presented in Part 1 of the GWQR submitted by Martin Marietta Energy Systems, Inc. (Energy Systems) to the Tennessee Department of Environment and Conservation (TDEC) in February 1994 (HSW Environmental Consultants, Inc. 1994a). Groundwater quality data evaluated in this report were obtained at several hazardous and non-hazardous waste management facilities and underground storage tanks (USTS) located within the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The Environmental Management Department of the Y-12 Plant Health, Safety, Environment, and Accountability Organization manages the groundwater monitoring activities in each regime under the auspices of the Y-12 Plant Groundwater Protection Program (GWPP). The purpose of the GWPP is to characterize the hydrogeology and to monitor groundwater quality at the Y-12 Plant and surrounding area to provide for protection of groundwater resources consistent with federal, state, and local requirements and in accordance with DOE Orders and Energy Systems corporate policy. The annual GWQR for the East Fork Regime is completed in two parts. Part 1 consists primarily of data appendices and serves as a reference for the groundwater quality data obtained each CY under the lead of the Y-12 Plant GWPP. Part 2 (this report) contains an evaluation of the data with respect to regime-wide groundwater quality, presents the findings and status of ongoing hydrogeologic studies, describes changes in monitoring priorities, and presents planned modifications to the groundwater sampling and analysis program for the following calendar year

  13. Y-12 Groundwater Protection Program Calendar Year 2000 Groundwater Monitoring Data Evaluation Report for the Upper East Fork Poplar Creek Hydrogeologic Regime at the U.S. Department of Energy Y-12 National Security Complex, Oak Ridge, Tennessee; FINAL

    International Nuclear Information System (INIS)

    None

    2001-01-01

    This report presents an evaluation of the groundwater and surface water monitoring data obtained during calendar year (CY) 2000 in the Upper East Fork Poplar Creek Hydrogeologic Regime (East Fork Regime). The East Fork Regime encompasses many confirmed and potential sources of groundwater and surface water contamination associated with the U.S. Department of Energy (DOE) Y-12 National Security Complex (hereafter referenced as Y-12) in Oak Ridge, Tennessee. Prepared under the auspices of the Y-12 Groundwater Protection Program (GWPP), this report addresses applicable provisions of DOE Order 5400.1 (General Environmental Protection Program) that require: (1) an evaluation of the quantity and quality of groundwater and surface water in areas that are, or could be, affected by Y-12 operations, (2) an evaluation of groundwater and surface water quality in areas where contaminants from Y-12 operations are most likely to migrate beyond the DOE Oak Ridge Reservation (ORR) property line, and (3) an evaluation of long-term trends in groundwater quality at Y-12. The following sections of this report contain relevant background information (Section 2.0); describe the results of the respective data evaluations required under DOE Order 5400.1 (Section 3.0); summarize significant findings of each evaluation (Section 4.0); and list the technical reports and regulatory documents cited for more detailed information (Section 5.0). Illustrations (maps and trend graphs) are presented in Appendix A. Brief data summary tables referenced in each section are contained within the text; supplemental information and extensive data tables are provided in Appendix B

  14. Aging Perspectives

    OpenAIRE

    Theodore D Cosco; David Brehme; Nora Grigoruta; Lisa-Katrin Kaufmann; Liis Lemsalu; Ruth Meex; Angela Schuurmans; Neslihan Sener

    2014-01-01

    Despite the proliferation of successful ageing (SA) research, the literature is dominated by researcher-driven Anglophone conceptualisations. To date, lay perspectives of SA have not been examined in Europe or Turkey. The current study aims to conduct a mixed-methods examination of conceptualisations of SA in seven underrepresented countries. Using snowball sampling via social media sites, an online survey consisting of established closed-ended and open-ended items – translated into seven lan...

  15. Healthy ageing

    DEFF Research Database (Denmark)

    Harrison, Adrian Paul; Brüggemann, Dagmar Adeline; Bartels, Else Marie

    2009-01-01

    The study employed mechanical stretching in vitro of sections of abdominal aorta of elderly mice to investigate any benefits of oral treatment with alpha-ketoglutarate (AKG) on arterial elasticity. Eighteen female mice (50-weeks-old) were assigned to a control (2% w/v) Na2-AKG or (2% w/v) a Ca-AK...... investigation as a candidate for therapies targeting arterial stiffening with age....

  16. Golden Age

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      Sometimes, a moment can announce the end of an age. The gold market is like that. Within two transaction days, the gold slumped by 13%and saw a 25%tumble from the high point in August 2011. According to the classic investment theory, a 20%-above decline means the shift from“a bull”to“a bear”market.   The super bear market of gold has lasted a dozen years. But the bull-to-bear shift was completed within only 20 minutes. Wall Street’s analysts and ordinary Chinese people had different understandings to the golden age.   Expecting a bear market, Wall Street continuously dumped gold, resulting in a diving of gold price. Seeing the benefits, leisure Chinese madams made a gold rush, causing a slight bounce of gold price. On April 23, renowned investment bank Goldman Sachs suspended the short sale. So,“Chinese aunts”beating back Wall Street’s analysts became the hottest topic online for the time being.   What are the decisive factors for the crash of gold price? Will the gold market complete a real bull-to-bear shift? Will the golden age of gold be farther or nearer?

  17. Aging Perspectives

    Directory of Open Access Journals (Sweden)

    Theodore D Cosco

    2014-05-01

    Full Text Available Despite the proliferation of successful ageing (SA research, the literature is dominated by researcher-driven Anglophone conceptualisations. To date, lay perspectives of SA have not been examined in Europe or Turkey. The current study aims to conduct a mixed-methods examination of conceptualisations of SA in seven underrepresented countries. Using snowball sampling via social media sites, an online survey consisting of established closed-ended and open-ended items – translated into seven languages – was administered. Grounded theory methods and descriptive statistics were used to analyse qualitative and quantitative data, respectively.

  18. Aging Blepharoplasty

    Directory of Open Access Journals (Sweden)

    Inchang Cho

    2013-09-01

    Full Text Available In performing upper blepharoplasty in the elderly, looking younger and keeping the eyelidsharmonious with the rest of the face have to be achieved at the same time. The most importantgoal in upper blepharoplasty for aging is correcting the drooping upper eyelid skin, and inthis process, the surgeon may or may not create a double eyelid fold. The pros and cons haveto be fully discussed with the patient, but the author personally prefers creating a doublefold unless the patient refuses, because it is efficient in correcting and preventing furtherdrooping of the skin. In most patients, the brow is elevated to compensate for the droopingeyelid, and when the drooping is corrected, brow ptosis may ensue. The surgeon has to preparefor these consequences before performing the procedure, and estimate the exact amountof skin to be excised. In the elderly, the skin and the orbicularis oculi muscle is thin, with adecreased amount of subcutaneous fat and retro-orbicularis oculi fat, and in most cases,excision of the skin alone is enough to correct the deformity. Removing large portions ofsoft tissue may also prolong the recovery period. Unlike younger patients, the lower skinflap should not be stretched too much in the elderly, as it may create an aggressive lookingappearance. A few wrinkles in the lower flap should remain untouched to create a naturallook. In this article, the author’s own methods of performing an aging blepharoplasty aredescribed specifically, with a step-by-step guide and surgical tips.

  19. Aging Gracefully.

    Science.gov (United States)

    Waldorf, Heidi A

    2017-06-01

    At the Annual Meeting of the American Academy of Dermatology in 2016, I directed the symposium entitled ‘Aging Gracefully’ for the second time. The symposium’s primary learning objective was to give the audience a framework for managing aesthetic patients. In the last decade, there has been a significant increase in the options for and availability of noninvasive and minimally invasive rejuvenation. With that has been an associated surge in interest by physicians and patients. And, as the number of procedures across the globe increases, so too do the number of aesthetically poor outcomes. Most come from practitioners doing paint-by-numbers treatment. As experts in skin biology and treatment, dermatologists should practice the highest-level cosmetic as well as general dermatology. The faculty presented information to aid practitioners how best to approach patients and formulate a cosmetic plan. The faculty discussed important considerations based on patient gender, age, and skin type. Updated information on the science of cosmeceuticals and the benefits of early noninvasive intervention was presented as were next generation devices and alternatives to standard therapies. The faculty have kindly condensed their AAD presentations for this supplement. I thank L’Oréal and the Journal of Drugs in Dermatology for giving us the opportunity to present this information to a wider audience in this e-supplement. Heidi A. Waldorf MD Director of Laser & Cosmetic Dermatology, The Mount Sinai Hospital, New York, NY Icahn School of Medicine of Mount Sinai, New York, NY Waldorf Dermatology Aesthetics, Nanuet, NY

  20. Aging Blepharoplasty

    Directory of Open Access Journals (Sweden)

    Inchang Cho

    2013-09-01

    Full Text Available In performing upper blepharoplasty in the elderly, looking younger and keeping the eyelids harmonious with the rest of the face have to be achieved at the same time. The most important goal in upper blepharoplasty for aging is correcting the drooping upper eyelid skin, and in this process, the surgeon may or may not create a double eyelid fold. The pros and cons have to be fully discussed with the patient, but the author personally prefers creating a double fold unless the patient refuses, because it is efficient in correcting and preventing further drooping of the skin. In most patients, the brow is elevated to compensate for the drooping eyelid, and when the drooping is corrected, brow ptosis may ensue. The surgeon has to prepare for these consequences before performing the procedure, and estimate the exact amount of skin to be excised. In the elderly, the skin and the orbicularis oculi muscle is thin, with a decreased amount of subcutaneous fat and retro-orbicularis oculi fat, and in most cases, excision of the skin alone is enough to correct the deformity. Removing large portions of soft tissue may also prolong the recovery period. Unlike younger patients, the lower skin flap should not be stretched too much in the elderly, as it may create an aggressive looking appearance. A few wrinkles in the lower flap should remain untouched to create a natural look. In this article, the author's own methods of performing an aging blepharoplasty are described specifically, with a step-by-step guide and surgical tips.

  1. Recent Approaches to Modeling Transport of Mercury in Surface Water and Groundwater - Case Study in Upper East Fork Poplar Creek, Oak Ridge, TN - 13349

    International Nuclear Information System (INIS)

    Bostick, Kent; Daniel, Anamary; Tachiev, Georgio; Malek-Mohammadi, Siamak

    2013-01-01

    In this case study, groundwater/surface water modeling was used to determine efficacy of stabilization in place with hydrologic isolation for remediation of mercury contaminated areas in the Upper East Fork Poplar Creek (UEFPC) Watershed in Oak Ridge, TN. The modeling simulates the potential for mercury in soil to contaminate groundwater above industrial use risk standards and to contribute to surface water contamination. The modeling approach is unique in that it couples watershed hydrology with the total mercury transport and provides a tool for analysis of changes in mercury load related to daily precipitation, evaporation, and runoff from storms. The model also allows for simulation of colloidal transport of total mercury in surface water. Previous models for the watershed only simulated average yearly conditions and dissolved concentrations that are not sufficient for predicting mercury flux under variable flow conditions that control colloidal transport of mercury in the watershed. The transport of mercury from groundwater to surface water from mercury sources identified from information in the Oak Ridge Environmental Information System was simulated using a watershed scale model calibrated to match observed daily creek flow, total suspended solids and mercury fluxes. Mercury sources at the former Building 81-10 area, where mercury was previously retorted, were modeled using a telescopic refined mesh with boundary conditions extracted from the watershed model. Modeling on a watershed scale indicated that only source excavation for soils/sediment in the vicinity of UEFPC had any effect on mercury flux in surface water. The simulations showed that colloidal transport contributed 85 percent of the total mercury flux leaving the UEFPC watershed under high flow conditions. Simulation of dissolved mercury transport from liquid elemental mercury and adsorbed sources in soil at former Building 81-10 indicated that dissolved concentrations are orders of magnitude

  2. Recent Approaches to Modeling Transport of Mercury in Surface Water and Groundwater - Case Study in Upper East Fork Poplar Creek, Oak Ridge, TN - 13349

    Energy Technology Data Exchange (ETDEWEB)

    Bostick, Kent; Daniel, Anamary [Professional Project Services, Inc., Bethel Valley Road, Oak Ridge, TN, 37922 (United States); Tachiev, Georgio [Florida International University, Applied Research Center 10555 W. Flagler St., EC 2100 Miami Florida 33174 (United States); Malek-Mohammadi, Siamak [Bradley University, 413A Jobst Hall, Preoria, IL 61625 (United States)

    2013-07-01

    In this case study, groundwater/surface water modeling was used to determine efficacy of stabilization in place with hydrologic isolation for remediation of mercury contaminated areas in the Upper East Fork Poplar Creek (UEFPC) Watershed in Oak Ridge, TN. The modeling simulates the potential for mercury in soil to contaminate groundwater above industrial use risk standards and to contribute to surface water contamination. The modeling approach is unique in that it couples watershed hydrology with the total mercury transport and provides a tool for analysis of changes in mercury load related to daily precipitation, evaporation, and runoff from storms. The model also allows for simulation of colloidal transport of total mercury in surface water. Previous models for the watershed only simulated average yearly conditions and dissolved concentrations that are not sufficient for predicting mercury flux under variable flow conditions that control colloidal transport of mercury in the watershed. The transport of mercury from groundwater to surface water from mercury sources identified from information in the Oak Ridge Environmental Information System was simulated using a watershed scale model calibrated to match observed daily creek flow, total suspended solids and mercury fluxes. Mercury sources at the former Building 81-10 area, where mercury was previously retorted, were modeled using a telescopic refined mesh with boundary conditions extracted from the watershed model. Modeling on a watershed scale indicated that only source excavation for soils/sediment in the vicinity of UEFPC had any effect on mercury flux in surface water. The simulations showed that colloidal transport contributed 85 percent of the total mercury flux leaving the UEFPC watershed under high flow conditions. Simulation of dissolved mercury transport from liquid elemental mercury and adsorbed sources in soil at former Building 81-10 indicated that dissolved concentrations are orders of magnitude

  3. Replicative age induces mitotic recombination in the ribosomal RNA gene cluster of Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Derek L Lindstrom

    2011-03-01

    Full Text Available Somatic mutations contribute to the development of age-associated disease. In earlier work, we found that, at high frequency, aging Saccharomyces cerevisiae diploid cells produce daughters without mitochondrial DNA, leading to loss of respiration competence and increased loss of heterozygosity (LOH in the nuclear genome. Here we used the recently developed Mother Enrichment Program to ask whether aging cells that maintain the ability to produce respiration-competent daughters also experience increased genomic instability. We discovered that this population exhibits a distinct genomic instability phenotype that primarily affects the repeated ribosomal RNA gene array (rDNA array. As diploid cells passed their median replicative life span, recombination rates between rDNA arrays on homologous chromosomes progressively increased, resulting in mutational events that generated LOH at >300 contiguous open reading frames on the right arm of chromosome XII. We show that, while these recombination events were dependent on the replication fork block protein Fob1, the aging process that underlies this phenotype is Fob1-independent. Furthermore, we provide evidence that this aging process is not driven by mechanisms that modulate rDNA recombination in young cells, including loss of cohesion within the rDNA array or loss of Sir2 function. Instead, we suggest that the age-associated increase in rDNA recombination is a response to increasing DNA replication stress generated in aging cells.

  4. Characterization of salinity loads and selenium loads in the Smith Fork Creek region of the Lower Gunnison River Basin, western Colorado, 2008-2009

    Science.gov (United States)

    Richards, Rodney J.; Linard, Joshua I.; Hobza, Christopher M.

    2014-01-01

    The lower Gunnison River Basin of the Colorado River Basin has elevated salinity and selenium levels. The Colorado River Basin Salinity Control Act of June 24, 1974 (Public Law 93–320, amended by Public Law 98–569), authorized investigation of the Lower Gunnison Basin Unit Salinity Control Project by the U.S. Department of the Interior. The Bureau of Reclamation (Reclamation) and the Natural Resources Conservation Service are responsible for assessing and implementing measures to reduce salinity and selenium loading in the Colorado River Basin. Cost-sharing programs help farmers, ranchers, and canal companies improve the efficiency of water delivery systems and irrigation practices. The delivery systems (irrigation canals) have been identified as potential sources of seepage, which can contribute to salinity loading. Reclamation wants to identify seepage from irrigation systems in order to maximize the effectiveness of the various salinity-control methods, such as polyacrylamide lining and piping of irrigation canals programs. The U.S. Geological Survey, in cooperation with Reclamation, developed a study to characterize the salinity and selenium loading of seven subbasins in the Smith Fork Creek region and identify where control efforts can be maximized to reduce salinity and selenium loading. Total salinity loads ranged from 27.9±19.1 tons per year (t/yr) to 87,500±80,500 t/yr. The four natural subbasins—BkKm, RCG1, RCG2, and SF1—had total salinity loads of 27.9±19.1 t/yr, 371±248 t/yr, 2,180±1,590 t/yr, and 4,200±2,720 t/yr, respectively. The agriculturally influenced sites had salinity loads that ranged from 7,580±6,900 t/yr to 87,500±80,500 t/yr. Salinity loads for the subbasins AL1, B1, CK1, SF2, and SF3 were 7,580±6,900 t/yr; 28,300±26,700 t/yr; 48,700±36,100 t/yr; 87,500±80,900 t/yr; and 52,200±31,800 t/yr, respectively. The agricultural salinity load was separated into three components: tail water, deep percolation, and canal seepage

  5. Evaluation of the behavior and movement patterns of adult coho salmon and steelhead in the North Fork Toutle River, Washington, 2005-2009

    Science.gov (United States)

    Liedtke, Theresa L.; Kock, Tobias J.; Rondorf, Dennis W.

    2013-01-01

    The 1980 eruption of Mount St. Helens severely affected the North Fork Toutle River (hereafter Toutle River), Washington, and threatened anadromous salmon (Oncorhynchus spp.) populations in the basin. The Toutle River was further affected in 1989 when a sediment retention structure (SRS) was constructed to trap sediments in the upper basin. The SRS completely blocked upstream volitional passage, so a fish collection facility (FCF) was constructed to trap adult coho salmon (O. kisutch) and steelhead (O. mykiss) so they could be transported upstream of the SRS. The Washington Department of Fish and Wildlife (WDFW) has operated a trap-and-haul program since 1989 to transport coho salmon and steelhead into tributaries of the Toutle River, upstream of the SRS. Although this program has allowed wild coho salmon and steelhead populations to persist in the Toutle River basin, the trap-andhaul program has faced many challenges that may be limiting the effectiveness of the program. We conducted a multi-year evaluation during 2005–2009 to monitor tagged fish in the upper Toutle River to provide information on the movements and behavior of adult coho salmon and steelhead, and to evaluate the efficacy of the FCF. Radio-tagged coho salmon and steelhead were released: (1) in Toutle River tributaries to evaluate the behavior and movements of fish released as part of the trap-and-haul program; (2) between the FCF and SRS to determine if volitional upstream passage through the SRS spillway was possible; (3) in the sediment plain upstream of the SRS to determine if volitional passage through the sediment plain was possible; and (4) downstream of the FCF to evaluate the efficacy of the structure. We also deployed an acoustic camera in the FCF to monitor fish movements near the entrance to the FCF, and in the fish holding vault where coho salmon and steelhead are trapped. A total of 20 radio-tagged coho salmon and 10 radio-tagged steelhead were released into Alder and Hoffstadt

  6. The role of episodic fire-related debris flows on long-term (103-104) sediment yields in the Middle Fork Salmon River Watershed, in central Idaho

    Science.gov (United States)

    Riley, K. E.; Pierce, J. L.; Hopkins, A.

    2010-12-01

    Episodic fire-related debris flows contribute large amounts of sediment and large woody debris to streams. This study evaluates fire-related sedimentation from small steep tributaries of the Middle Fork Salmon River (MFSR) in central Idaho to evaluate the timing, frequency, and magnitude of episodic fire-related sedimentation on long-term (10 3-10 4) sediment yields. The MFSR lies within the Northern Rocky Mountains and encompasses a range of ecosystems including high elevation (~3,000 -1,700 m) subalpine pine and spruce forests, mid-elevation (2650 - 1130 m) montane Douglas-fir and ponderosa pine-dominated forests and low elevation (~ 1,800 - 900 m) sagebrush steppe. Recent debris flow events in tributaries of the MFSR appear to primarily result from increased surface runoff, rilling, and progressive sediment bulking following high severity fires. This study estimates: 1) the volume of sediment delivered by four recent (1997-2008) fire-related debris flow events using real time kinematic GPS surveys, and 2) the timing of Holocene fire-related debris flow events determined by 14C dating charcoal fragments preserved in buried burned soils and within fire-related deposits. Our measured volumes of the four recent debris flow events are compared to two empirically derived volume estimates based on remotely sensed spatial data (burn severity and slope), measured geometric data (longitudinal profile, cross sectional area, flow banking angle), and precipitation records. Preliminary stratigraphic profiles in incised alluvial fans suggest that a large percentage of alluvial fan thickness is composed of fire-related deposits suggesting fire-related hillslope erosion is a major process delivering sediment to alluvial fans and to the MFSR. Fire-related deposits from upper basins compose ~71% of total alluvial fan thickness, while fire-related deposits from lower basins make up 36% of alluvial fan thickness. However, lower basins are less densely vegetated with small diameter

  7. Fate and transport of petroleum hydrocarbons in soil and ground water at Big South Fork National River and Recreation Area, Tennessee and Kentucky, 2002-2003

    Science.gov (United States)

    Williams, Shannon D.; Ladd, David E.; Farmer, James

    2006-01-01

    In 2002 and 2003, the U.S. Geological Survey (USGS), by agreement with the National Park Service (NPS), investigated the effects of oil and gas production operations on ground-water quality at Big South Fork National River and Recreation Area (BISO) with particular emphasis on the fate and transport of petroleum hydrocarbons in soils and ground water. During a reconnaissance of ground-water-quality conditions, samples were collected from 24 different locations (17 springs, 5 water-supply wells, 1 small stream, and 1 spring-fed pond) in and near BISO. Benzene, toluene, ethylbenzene, and xylene (BTEX) compounds were not detected in any of the water samples, indicating that no widespread contamination of ground-water resources by dissolved petroleum hydrocarbons probably exists at BISO. Additional water-quality samples were collected from three springs and two wells for more detailed analyses to obtain additional information on ambient water-quality conditions at BISO. Soil gas, soil, water, and crude oil samples were collected at three study sites in or near BISO where crude oil had been spilled or released (before 1993). Diesel range organics (DRO) were detected in soil samples from all three of the sites at concentrations greater than 2,000 milligrams per kilogram. Low concentrations (less than 10 micrograms per kilogram) of BTEX compounds were detected in lab-analyzed soil samples from two of the sites. Hydrocarbon-degrading bacteria counts in soil samples from the most contaminated areas of the sites were not greater than counts for soil samples from uncontaminated (background) sites. The elevated DRO concentrations, the presence of BTEX compounds, and the low number of -hydrocarbon-degrading bacteria in contaminated soils indicate that biodegradation of petroleum hydrocarbons in soils at these sites is incomplete. Water samples collected from the three study sites were analyzed for BTEX and DRO. Ground-water samples were collected from three small springs at the

  8. Field Comparisons of the Elwha Bedload Sampler and an Acoustic Gravel-transport Sensor: Middle Fork of the Piedra River, Colorado, USA

    Science.gov (United States)

    Downing, J.; Ryan, S. E.

    2001-12-01

    Ten simultaneous bedload measurements were made with an Elhwa sampler and an acoustic-gravel-transport sensor (GTS) on the Middle Fork of the Piedra River in southwestern Colorado near the end of the spring freshet in water year 2001. The purpose was to compare bedload samples with acoustic measurements acquired under field conditions. Upstream of the measurement site, the river drains 86 km2 of andesite, ash flows, tuffs, and breccias in the San Juan Mountains, contributing a relatively high sediment load to the river system. The channel transitions from step-pools at high elevations to a plane bed with a slope of 0.018 in the study reach. Channel width, mean depth and bank-full velocity at the site are: 13.6 m, 0.52, and 1.5 m s-1. The D50 of the riverbed surface is 0.08 m which is 6 to 40 times larger than the D50s of the bedload samples. D16 and D84 of the bed = 0.02 and 0.21 m respectively. Water discharges from 7.3 to 9.3 m3 s-1 transported about 0.01 kg of gravel m-1 s-1 in the channel. Transport of coarse gravel (8-64 mm) ranged from 0.00063 to 0.024 kg m-1 s-1. The Elwha sampler is a portable, pressure-differential trap with a 0.2 m wide by 0.1 m high opening. The acoustic sensor is a 0.025-m wide by 0.1 m high strip of PVDF piezoelectric film connected to a signal processor and bonded to an aluminum pressure plate. When the plate is struck by stones, the GTS produces signal peaks with areas that are accurate measures of stone momentum. The GTS was calibrated with steels balls dropped on the pressure plate in still water to develop a curve of ball momentum as a function of peak areas. Based on these calibrations, the standard error of the GTS momentum estimates is 0.0017 kg m s-1. Five transects with 30 verticals, each occupied for 60 s, were completed with the sampler and GTS separated by < 1 m. Five additional verticals were occupied for about 1800 s each with the instruments separated by < 0.5 m. The trapped material was sieved and weighed and the water

  9. Evaluation of LiDAR-acquired bathymetric and topographic data accuracy in various hydrogeomorphic settings in the Deadwood and South Fork Boise Rivers, West-Central Idaho, 2007

    Science.gov (United States)

    Skinner, Kenneth D.

    2011-01-01

    High-quality elevation data in riverine environments are important for fisheries management applications and the accuracy of such data needs to be determined for its proper application. The Experimental Advanced Airborne Research LiDAR (Light Detection and Ranging)-or EAARL-system was used to obtain topographic and bathymetric data along the Deadwood and South Fork Boise Rivers in west-central Idaho. The EAARL data were post-processed into bare earth and bathymetric raster and point datasets. Concurrently with the EAARL surveys, real-time kinematic global positioning system surveys were made in three areas along each of the rivers to assess the accuracy of the EAARL elevation data in different hydrogeomorphic settings. The accuracies of the EAARL-derived raster elevation values, determined in open, flat terrain, to provide an optimal vertical comparison surface, had root mean square errors ranging from 0.134 to 0.347 m. Accuracies in the elevation values for the stream hydrogeomorphic settings had root mean square errors ranging from 0.251 to 0.782 m. The greater root mean square errors for the latter data are the result of complex hydrogeomorphic environments within the streams, such as submerged aquatic macrophytes and air bubble entrainment; and those along the banks, such as boulders, woody debris, and steep slopes. These complex environments reduce the accuracy of EAARL bathymetric and topographic measurements. Steep banks emphasize the horizontal location discrepancies between the EAARL and ground-survey data and may not be good representations of vertical accuracy. The EAARL point to ground-survey comparisons produced results with slightly higher but similar root mean square errors than those for the EAARL raster to ground-survey comparisons, emphasizing the minimized horizontal offset by using interpolated values from the raster dataset at the exact location of the ground-survey point as opposed to an actual EAARL point within a 1-meter distance. The

  10. Grand Forks - East Grand Forks Urban Water Resources Study. Grand Forks Flood Fight Manual

    Science.gov (United States)

    1981-07-01

    insurance is available for residential and commercial structures and contents at actuarial rates. As of 30 April 1980, 1258 policies were in effect...STATION #3 (WW) CLOSE SHEAR OATE ON LEW« M.VD. ON RIVER SOE OF IMKE. (S) PUCE PUMP M CATCH ■ ASM ON OTHER «DC OF MKE CLOSE SHEAR OATE ON OKE AT ISIS mVBMM...DRIVE. (S>* PUCE PUMP M CATCH « ASM ON OTHER SBC OF (NKE CLOU SHEAR OATE AT UFT STATION «S M MANHOU «WW> SWITCH OVER UFT STATION «I - CLOSE OATE

  11. Proposed modifications to the RCRA post-closure permit for the Upper East Fork Poplar Creek Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    1997-05-01

    This report presents proposed modifications to the Resource Conservation and Recovery Act (RCRA) Post-Closure Permit (PCP) for the Upper East Fork Poplar Creek Hydrogeologic Regime (permit number TNHW-088, EPA ID No. TN3 89 009 0001). The modifications are proposed to: (1) revise the current text for two of the Permit Conditions included in Permit Section II - General Facility Conditions, and (2) update the PCP with revised versions of the Y-12 Plant Groundwater Protection Program (GWPP) technical field procedures included in several of the Permit Attachments. The updated field procedures and editorial revisions are Class 1 permit modifications, as specified in Title 40, Code of Federal Regulations (CFR) section 270.42; Appendix I - Classification of Permit Modifications. These modifications are summarized below

  12. Proposed modifications to the RCRA post-closure permit for the Upper East Fork Poplar Creek Hydrogeologic Regime at the U.S. Department of Energy Y-12 Plant, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    This report presents proposed modifications to the Resource Conservation and Recovery Act (RCRA) Post-Closure Permit (PCP) for the Upper East Fork Poplar Creek Hydrogeologic Regime (permit number TNHW-088, EPA ID No. TN3 89 009 0001). The modifications are proposed to: (1) revise the current text for two of the Permit Conditions included in Permit Section II - General Facility Conditions, and (2) update the PCP with revised versions of the Y-12 Plant Groundwater Protection Program (GWPP) technical field procedures included in several of the Permit Attachments. The updated field procedures and editorial revisions are Class 1 permit modifications, as specified in Title 40, Code of Federal Regulations (CFR) {section}270.42; Appendix I - Classification of Permit Modifications. These modifications are summarized below.

  13. Summary of surface-water-quality data collected for the Northern Rockies Intermontane Basins National Water-Quality Assessment Program in the Clark Fork-Pend Oreille and Spokane River basins, Montana, Idaho, and Washington, water years 1999-2001

    Science.gov (United States)

    Beckwith, Michael A.

    2003-01-01

    Water-quality samples were collected at 10 sites in the Clark Fork-Pend Oreille and Spokane River Basins in water years 1999 – 2001 as part of the Northern Rockies Intermontane Basins (NROK) National Water-Quality Assessment (NAWQA) Program. Sampling sites were located in varied environments ranging from small streams and rivers in forested, mountainous headwater areas to large rivers draining diverse landscapes. Two sampling sites were located immediately downstream from the large lakes; five sites were located downstream from large-scale historical mining and oreprocessing areas, which are now the two largest “Superfund” (environmental remediation) sites in the Nation. Samples were collected during a wide range of streamflow conditions, more frequently during increasing and high streamflow and less frequently during receding and base-flow conditions. Sample analyses emphasized major ions, nutrients, and selected trace elements. Streamflow during the study ranged from more than 130 percent of the long-term average in 1999 at some sites to 40 percent of the long-term average in 2001. River and stream water in the study area exhibited small values for specific conductance, hardness, alkalinity, and dissolved solids. Dissolved oxygen concentrations in almost all samples were near saturation. Median total nitrogen and total phosphorus concentrations in samples from most sites were smaller than median concentrations reported for many national programs and other NAWQA Program study areas. The only exceptions were two sites downstream from large wastewater-treatment facilities, where median concentrations of total nitrogen exceeded the national median. Maximum concentrations of total phosphorus in samples from six sites exceeded the 0.1 milligram per liter threshold recommended for limiting nuisance aquatic growth. Concentrations of arsenic, cadmium, copper, lead, mercury, and zinc were largest in samples from sites downstream from historical mining and ore

  14. A Novel Rrm3 Function in Restricting DNA Replication via an Orc5-Binding Domain Is Genetically Separable from Rrm3 Function as an ATPase/Helicase in Facilitating Fork Progression

    Science.gov (United States)

    Syed, Salahuddin; Desler, Claus; Rasmussen, Lene J.; Schmidt, Kristina H.

    2016-01-01

    In response to replication stress cells activate the intra-S checkpoint, induce DNA repair pathways, increase nucleotide levels, and inhibit origin firing. Here, we report that Rrm3 associates with a subset of replication origins and controls DNA synthesis during replication stress. The N-terminal domain required for control of DNA synthesis maps to residues 186–212 that are also critical for binding Orc5 of the origin recognition complex. Deletion of this domain is lethal to cells lacking the replication checkpoint mediator Mrc1 and leads to mutations upon exposure to the replication stressor hydroxyurea. This novel Rrm3 function is independent of its established role as an ATPase/helicase in facilitating replication fork progression through polymerase blocking obstacles. Using quantitative mass spectrometry and genetic analyses, we find that the homologous recombination factor Rdh54 and Rad5-dependent error-free DNA damage bypass act as independent mechanisms on DNA lesions that arise when Rrm3 catalytic activity is disrupted whereas these mechanisms are dispensable for DNA damage tolerance when the replication function is disrupted, indicating that the DNA lesions generated by the loss of each Rrm3 function are distinct. Although both lesion types activate the DNA-damage checkpoint, we find that the resultant increase in nucleotide levels is not sufficient for continued DNA synthesis under replication stress. Together, our findings suggest a role of Rrm3, via its Orc5-binding domain, in restricting DNA synthesis that is genetically and physically separable from its established catalytic role in facilitating fork progression through replication blocks. PMID:27923055

  15. A Novel Rrm3 Function in Restricting DNA Replication via an Orc5-Binding Domain Is Genetically Separable from Rrm3 Function as an ATPase/Helicase in Facilitating Fork Progression.

    Science.gov (United States)

    Syed, Salahuddin; Desler, Claus; Rasmussen, Lene J; Schmidt, Kristina H

    2016-12-01

    In response to replication stress cells activate the intra-S checkpoint, induce DNA repair pathways, increase nucleotide levels, and inhibit origin firing. Here, we report that Rrm3 associates with a subset of replication origins and controls DNA synthesis during replication stress. The N-terminal domain required for control of DNA synthesis maps to residues 186-212 that are also critical for binding Orc5 of the origin recognition complex. Deletion of this domain is lethal to cells lacking the replication checkpoint mediator Mrc1 and leads to mutations upon exposure to the replication stressor hydroxyurea. This novel Rrm3 function is independent of its established role as an ATPase/helicase in facilitating replication fork progression through polymerase blocking obstacles. Using quantitative mass spectrometry and genetic analyses, we find that the homologous recombination factor Rdh54 and Rad5-dependent error-free DNA damage bypass act as independent mechanisms on DNA lesions that arise when Rrm3 catalytic activity is disrupted whereas these mechanisms are dispensable for DNA damage tolerance when the replication function is disrupted, indicating that the DNA lesions generated by the loss of each Rrm3 function are distinct. Although both lesion types activate the DNA-damage checkpoint, we find that the resultant increase in nucleotide levels is not sufficient for continued DNA synthesis under replication stress. Together, our findings suggest a role of Rrm3, via its Orc5-binding domain, in restricting DNA synthesis that is genetically and physically separable from its established catalytic role in facilitating fork progression through replication blocks.

  16. Long-Term Movement and Estimated Age of a Paddlefish (Polyodon spathula) in the Arkansas River Basin of Oklahoma

    Science.gov (United States)

    Long, James M.

    2018-01-01

    We report the age and distance moved for an individual paddlefish (Polyodon spathula) that was tagged March 1998 in the Cimarron River Arm of Keystone Lake, Oklahoma, and snagged by an angler in April 2016 downstream of Eufaula Dam, Oklahoma. The fish was part of a cohort spawned in 1995. At the time of initial capture, the fish measured 795 mm eye–fork length, was estimated to be 3 y old, and 18 y had elapsed before its recapture by an angler in 2016, indicating this fish was 21 y old at recapture. Although paddlefish as old as 27 have been estimated in the Grand River basin of Oklahoma, this is the oldest fish known in the Arkansas River basin of Oklahoma. At the place of its recapture, this fish would have traveled approximately 235 km, passing downstream through three dams before moving upstream to Eufaula Dam.

  17. Oral Health and Aging

    Science.gov (United States)

    ... please turn JavaScript on. Feature: Oral Health and Aging Oral Health and Aging Past Issues / Summer 2016 Table of Contents Jerrold ... they may need. Read More "Oral Health and Aging" Articles Oral Health and Aging / 4 Myths About ...

  18. Age, growth, and natural mortality of schoolmaster (Lutjanus apodus from the southeastern United States

    Directory of Open Access Journals (Sweden)

    Jennifer C. Potts

    2016-10-01

    Full Text Available Ages of schoolmaster (n = 136 from the southeastern Florida coast from 1981–2015 were determined using sectioned sagittal otoliths. Opaque zones were annular, forming March–July (peaking in May–June. Schoolmaster ranged in age from 1–42 years; the largest fish measured 505 mm total length (TL and was 19 years old. The oldest fish measured 440 mm TL. Estimated body size relationships for schoolmaster were: W = 9.26 × 10−6 TL3.11 (n = 256, r2 = 0.95; W = 2.13 × 10−5 FL2.99 (n = 161, r2 = 0.95; TL = 1.03 FL + 10.36 (n = 143, r2 = 0.99; and FL = 0.96 TL − 8.41 (n = 143, r2 = 0.99, where W = whole weight in g, FL = fork length in mm, and TL in mm. The fitted von Bertalanffy growth equation was: Lt = 482 (1 − e−0.12(t+2.79 (n = 136. Based on published life history relationships, a point estimate of natural mortality for schoolmaster was M = 0.10, while age-specific estimates of M ranged from 1.57–0.18 for ages 1–42.

  19. Age and osteoarthritis: are AGEs the link?

    NARCIS (Netherlands)

    Vos, P.A.J.M.

    2012-01-01

    Osteoarthritis is a common joint disease in the elderly. By far the most important risk factor for osteoarthritis is aging. Age-related changes in the articular cartilage may be responsible for age-related increase in the number of people with osteoarthritis. One of the most striking changes in

  20. How Low Can You Go? Determining a Size Threshold for Implantation of a New Acoustic Transmitter in Age-0 White Sturgeon

    Energy Technology Data Exchange (ETDEWEB)

    Ashton, Neil K.; Liss, Stephanie A.; Walker, Ricardo W.; Brown, Richard S.; Klassen, Cheryl; Backhouse, Stephanie; Bates, Phil; Townsend, Richard L.

    2017-01-01

    Telemetry studies are often used to investigate sturgeon habitat use and movement patterns; however, existing acoustic transmitters are generally too large to implant into age-0 sturgeon without harming the fish. Recent development of a miniaturized acoustic transmitter (cylindrical, 0.7 g in air, 24.2 mm long, 5.0 mm diameter) with up to 365 d battery life has the potential to advance our understanding of age-0 sturgeon ecology in rivers and lakes. Prior to use in field studies, it is essential to conduct experiments evaluating potential adverse transmitter effects on fish. We tested transmitter retention, fish survival, and growth of a broad size range of age-0 white sturgeon (Acipenser transmontanus; 158–277 mm fork length; 26–126 g; 0.6–2.6% transmitter burden) in an 84 d laboratory study, with an ultimate goal of determining a minimum size threshold of sturgeon that can be implanted with this acoustic transmitter. At 84 d post-implantation, transmitter retention and fish survival were 100%. Specific growth rates were reduced at 7 and 14 d post-implantation, resulting in minimum fork length thresholds of 250 and 171 mm, respectively. Juveniles implanted with transmitters regained their growth potential by 28 d post-implantation and no size differences were detected in comparisons with unmarked control fish. This study demonstrates the ability to implant small age-0 sturgeon with high transmitter retention and fish survival, and only minor growth effects. Use of new miniaturized acoustic transmitters may give researchers a means to address questions about young-of-the-year fish recruitment, ecological patterns, and potentially advance conservation management of sturgeon populations.

  1. Effects of the Upper Taum Sauk Reservoir Embankment Breach on the Surface-Water Quality and Sediments of the East Fork Black River and the Black River, Southeastern Missouri - 2006-07

    Science.gov (United States)

    Barr, Miya N.

    2009-01-01

    On December 14, 2005, a 680-foot wide section of the upper reservoir embankment of the Taum Sauk pump-storage hydroelectric powerplant located in Reynolds County, Missouri, suddenly failed. This catastrophic event sent approximately 1.5 billion gallons of water into the Johnson's Shut-Ins State Park and into the East Fork Black River, and deposited enormous quantities of rock, soil, and vegetation in the flooded areas. Water-quality data were collected within and below the impacted area to study and document the changes to the riverene system. Data collection included routine, event-based, and continuous surface-water quality monitoring as well as suspended- and streambed-sediment sampling. Surface water-quality samples were collected and analyzed for a suite of physical and chemical constituents including: turbidity; nutrients; major ions such as calcium, magnesium, and potassium; total suspended solids; total dissolved solids; trace metals such as aluminum, iron, and lead; and suspended-sediment concentrations. Suspended-sediment concentrations were used to calculate daily sediment discharge. A peculiar blue-green coloration on the water surface of the East Fork Black River and Black River was evident downstream from the lower reservoir during the first year of the study. It is possible that this phenomenon was the result of 'rock flour' occurring when the upper reservoir embankment was breached, scouring the mountainside and producing extremely fine sediment particles, or from the alum-based flocculent used to reduce turbidity in the lower reservoir. It also was determined that no long-term effects of the reservoir embankment breach are expected as the turbidity and concentrations of trace metals such as total recoverable aluminum, dissolved aluminum, dissolved iron, and suspended-sediment concentration graphically decreased over time. Larger concentrations of these constituents during the beginning of the study also could be a direct result of the alum

  2. Corrected Age for Preemies

    Science.gov (United States)

    ... Our Sponsors Ages & Stages Ages & Stages Ages and Stages Prenatal Baby (0-12 mos.) Toddler 1-3yrs. Preschool 3-5yrs Grade School 5-12yrs. Teen 12- ... the Word Shop AAP Find a Pediatrician Ages & Stages Prenatal Baby ... Gradeschool Teen Young Adult Healthy Children > Ages & Stages > ...

  3. Nutrients, Microglia Aging, and Brain Aging

    Directory of Open Access Journals (Sweden)

    Zhou Wu

    2016-01-01

    Full Text Available As the life expectancy continues to increase, the cognitive decline associated with Alzheimer’s disease (AD becomes a big major issue in the world. After cellular activation upon systemic inflammation, microglia, the resident immune cells in the brain, start to release proinflammatory mediators to trigger neuroinflammation. We have found that chronic systemic inflammatory challenges induce differential age-dependent microglial responses, which are in line with the impairment of learning and memory, even in middle-aged animals. We thus raise the concept of “microglia aging.” This concept is based on the fact that microglia are the key contributor to the acceleration of cognitive decline, which is the major sign of brain aging. On the other hand, inflammation induces oxidative stress and DNA damage, which leads to the overproduction of reactive oxygen species by the numerous types of cells, including macrophages and microglia. Oxidative stress-damaged cells successively produce larger amounts of inflammatory mediators to promote microglia aging. Nutrients are necessary for maintaining general health, including the health of brain. The intake of antioxidant nutrients reduces both systemic inflammation and neuroinflammation and thus reduces cognitive decline during aging. We herein review our microglia aging concept and discuss systemic inflammation and microglia aging. We propose that a nutritional approach to controlling microglia aging will open a new window for healthy brain aging.

  4. Nutrients, Microglia Aging, and Brain Aging.

    Science.gov (United States)

    Wu, Zhou; Yu, Janchun; Zhu, Aiqin; Nakanishi, Hiroshi

    2016-01-01

    As the life expectancy continues to increase, the cognitive decline associated with Alzheimer's disease (AD) becomes a big major issue in the world. After cellular activation upon systemic inflammation, microglia, the resident immune cells in the brain, start to release proinflammatory mediators to trigger neuroinflammation. We have found that chronic systemic inflammatory challenges induce differential age-dependent microglial responses, which are in line with the impairment of learning and memory, even in middle-aged animals. We thus raise the concept of "microglia aging." This concept is based on the fact that microglia are the key contributor to the acceleration of cognitive decline, which is the major sign of brain aging. On the other hand, inflammation induces oxidative stress and DNA damage, which leads to the overproduction of reactive oxygen species by the numerous types of cells, including macrophages and microglia. Oxidative stress-damaged cells successively produce larger amounts of inflammatory mediators to promote microglia aging. Nutrients are necessary for maintaining general health, including the health of brain. The intake of antioxidant nutrients reduces both systemic inflammation and neuroinflammation and thus reduces cognitive decline during aging. We herein review our microglia aging concept and discuss systemic inflammation and microglia aging. We propose that a nutritional approach to controlling microglia aging will open a new window for healthy brain aging.

  5. PerformAge

    DEFF Research Database (Denmark)

    Fristrup, Tine

    2012-01-01

    are constituted as experts on aging. In the humanistic model of aging, the aging self becomes the expert on the process of becoming of age. Thi sconflicting aspect of the discursive battlefield on aging will be elaborated in the framework of PerformAge and understood as a temporal and spatial theorising of aging......: the poetics of aging. Making the lived experience the centrepiece of this paper, poetic representations of aging lives (Laurel Richardson, 1991, 1992) will articulate subjectively felt experiences in social encounters between people performing age. In writing the social (Dorothy E. Smith, 1999) encounters, I...... as a researcher perform age in the process of figuring the possible age performativities as subject positioning performativity (Judith Butler, 1995) in the social encounters and textualized in poetic representations. Inspired by the work on Reifungsromane (Barbara Frey Waxman, 1990), I do not want to paint...

  6. New concept of age(ing: Prospective age

    Directory of Open Access Journals (Sweden)

    Devedžić Mirjana

    2012-01-01

    Full Text Available While the last century was the century of world population growth, according to demographers, the XXI century will be century of population aging. Statistics undoubtedly show that number of elderly will continue it’s growth in the future. If old age is seen as period of life with reduced physical and mental capabilities and increased disability, and demographic aging as increase of dependent population, trends are quite disturbing, at least in certain societal segments. In developed countries, this population category is no longer treated as passive or as a "burden of society" and efforts are made for better social inclusion of older people. In contrast to growing interest in this phenomenon, the concepts that define the aging of the population remained stagnant. The aim of this paper is to introduce into domestic literature the term "prospective age" as a dynamic category which is more affected with socio-historical conditions, not only with biological as traditional definition of aging suggested. Papers written by Sanderson and Scherbov offer new methodological options for study of population aging, because it takes into account the biometric rather than chronological approach. Calculation of prospective years is a simple operation that requires pair of the same number of remained life expectancy from life tables for two different periods (the year of concern is index, and the one we are comparing with is standard year, so that phrase "40s is the new 30s" or "70s the new 60s" gets scientific foundation. Average remaining years of life represent a realistic indicator suggesting increased capacity, activity and vitality of individuals, which is due to accepted demographic parameters still considered old. „Prospective threshold“ is defined as the age when life expectancy falls below 15 years (it is subjective choice made by Sanderson and Scherbov, which is also used in this paper and during the elaboration of these ideas three demographic

  7. Aerial gamma ray and magnetic survey: Minnesota Project, Thief River Falls, Grand Forks, Fargo, Milbank, Watertown, New Ulm and St. Cloud quadrangles of North Dakota, South Dakota and Minnesota. Final report

    International Nuclear Information System (INIS)

    1979-10-01

    During the months of August and September 1979, geoMetrics, Inc., collected 12,415 line miles of high sensitivity airborne radiometric and magnetic data in adjoining portions of South Dakota and Minnesota over seven 1 by 2 degree NTMS quadrangles (Thief River Falls, Grand Forks, Fargo, Milbank, Watertown, New Ulm, and St. Cloud) as part of the Department of Energy's National Uranium Resource Evaluation Program. All radiometric and magnetic data were fully corrected and interpreted by geoMetrics and are presented as eight volumes (one Volume I and seven Volume II's). Regional geology for these seven quadrangles can be divided into two logical sections. The first comprises the surficial glacial deposits, which mantle most of the area and can be up to hundreds of feet thick. The second section consists of the underlying bedrock which is exposed in small scattered outcrops, generally along major drainages. No sedimentary structures exist within the quadrangles. As of this writing, no known uranium deposits exist within the seven quadrangles

  8. Distribution of metals during digestion by cutthroat trout fed benthic invertebrates contaminated in the Clark Fork River, Montana and the Coeur d'Alene River, Idaho, U.S.A., and fed artificially contaminated Artemia

    Science.gov (United States)

    Farag, A.M.; Suedkamp, M.J.; Meyer, J.S.; Barrows, R.; Woodward, D.F.

    2000-01-01

    The concentrations of essential amino acids in three, undigested invertebrate diets collected from the Clark Fork River (CFR) for cutthroat trout were similar to each other, but were c. 25–75% less than Artemia that were exposed to a mixture of arsenic, copper, cadmium, lead and zinc in the laboratory. The Artemia diet appeared less palatable and the texture, quantity and appearance of the intestinal contents differed between fish fed the Artemia and CFR diets. The Pb% in the fluid fraction of the intestinal contents was greater for the Artemia (29%) than for the CFR diets (10–17%), and the Cu% in the amino acid plus metal fraction of the intestinal contents was greater for the Artemia (78%) than for two of the three CFR diets (67% and 70%). Intestinal contents of fish fed invertebrate diets collected from various sites on the Coeur d'Alene River (CDA), Idaho, were similar in texture, quantity, and appearance. For fish fed the CDA diets, differences in the distribution of metals among fractions of the digestive fluids appeared to be related to concentrations of metals in the invertebrate diets. Pb% was lowest of all metals in the fluid portion of the intestinal contents. However, >80% of all metals in the hind gut were associated with the particulate fraction where they may still be available for uptake through pinocytosis.

  9. Large self-biased and multi-peak magnetoelectric coupling in transducer of Pb(Zr,Ti)O3 plates and H-type magnetization-graded ferromagnetic fork

    Science.gov (United States)

    Shen, Yongchun; Ling, Zhihao; Lu, Caijiang

    2015-12-01

    This paper develops a self-biased magnetoelectric (ME) composite Metglas/H-type-FeNi/PZT (MHFP) of H-type magnetization-graded Metglas/H-type-FeNi fork and piezoelectric Pb(Zr,Ti)O3 (PZT) plate. By using the magnetization-graded magnetostrictive layer and symmetrical H-type structure, giant self-biased ME coupling and multi-peak phenomenon are observed. The zero-biased ME voltage coefficient of MHFP composite reaches ˜63.8 V/cm Oe, which is ˜37.5 times higher than that of traditional FeNi/PZT laminate. The output ME voltage has a good near linear relation with Hac and is determined to be ˜5.1 V/Oe and ˜10.6 mV/Oe at ˜65 kHz and 1 kHz, respectively. These indicate that the proposed composite show promising applications for ME transducers and high-sensitivity self-biased magnetic sensors.

  10. Hemostasis and ageing

    OpenAIRE

    Mari, D.; Ogliari, G.; Castaldi, D.; Vitale, G.; Bollini, E.M.; Lio, D.

    2008-01-01

    Abstract On March 19, 2008 a Symposium on Pathophysiology of Ageing and Age-Related Diseases was held in Palermo, Italy. The lecture of D. Mari on Hemostasis and ageing is summarized herein. Physiological ageing is associated with increased plasma levels of many proteins of blood coagulation together with fibrinolysis impairment. This may be of great concern in view of the known association between vascular and thromboembolic diseases and ageing. On the other hand, centenarians are characteri...

  11. Heart Age PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    This 60 second public service announcement is based on the September 2015 CDC Vital Signs report. Your heart age is the age of your heart and blood vessels as a result of your risk factors for heart attack and stroke. If you smoke or have high blood pressure, your heart age will be much higher than your actual age. Learn what you can do to lower your heart age and keep it low.

  12. The Aging Epigenome

    Science.gov (United States)

    Booth, Lauren N.

    2016-01-01

    During aging, the mechanisms that normally maintain health and stress resistance strikingly decline, resulting in decrepitude, frailty, and ultimately death. Exactly when and how this decline occurs is unknown. Changes in transcriptional networks and chromatin state lie at the heart of age-dependent decline. These epigenomic changes are not only observed during aging but also profoundly affect cellular function and stress resistance, thereby contributing to the progression of aging. We propose that the dysregulation of transcriptional and chromatin networks is a crucial component of aging. Understanding age-dependent epigenomic changes will yield key insights into how aging begins and progresses and should lead to the development of new therapeutics that delay or even reverse aging and age-related diseases. PMID:27259204

  13. Epigenetic predictor of age.

    Directory of Open Access Journals (Sweden)

    Sven Bocklandt

    Full Text Available From the moment of conception, we begin to age. A decay of cellular structures, gene regulation, and DNA sequence ages cells and organisms. DNA methylation patterns change with increasing age and contribute to age related disease. Here we identify 88 sites in or near 80 genes for which the degree of cytosine methylation is significantly correlated with age in saliva of 34 male identical twin pairs between 21 and 55 years of age. Furthermore, we validated sites in the promoters of three genes and replicated our results in a general population sample of 31 males and 29 females between 18 and 70 years of age. The methylation of three sites--in the promoters of the EDARADD, TOM1L1, and NPTX2 genes--is linear with age over a range of five decades. Using just two cytosines from these loci, we built a regression model that explained 73% of the variance in age, and is able to predict the age of an individual with an average accuracy of 5.2 years. In forensic science, such a model could estimate the age of a person, based on a biological sample alone. Furthermore, a measurement of relevant sites in the genome could be a tool in routine medical screening to predict the risk of age-related diseases and to tailor interventions based on the epigenetic bio-age instead of the chronological age.

  14. Age validation and variation in growth, mortality and population structure of Liza argentea and Myxus elongatus (Mugilidae) in two temperate Australian estuaries.

    Science.gov (United States)

    Kendall, B W; Gray, C A; Bucher, D

    2009-12-01

    This study investigated variation in the rates of growth and mortality, and age and fork-length (L(F)) compositions of two exploited species of Mugilidae, Liza argentea and Myxus elongatus, in two south-east Australian estuaries (Lake Macquarie and St Georges Basin). An ageing protocol was developed by counting opaque growth zones on sectioned otoliths which was validated by periodically examining the otoliths of captive-reared young-of-the-year fishes, and marginal increment analysis of wild fishes. The maximum recorded age was 17 years for L. argentea and 12 years for M. elongatus, which is greater than generally observed in other species of mugilids. Growth models of each species significantly differed between sexes and, except for male L. argentea, between estuaries. Fishes from Lake Macquarie generally had a greater mean L(F) at age than those from St Georges Basin and females of both species generally attained a greater maximum L(F) and age than males. Gillnet catches of L. argentea were of similar L(F) and age compositions in both estuaries, whereas the age composition of catches of M. elongatus in Lake Macquarie contained a greater proportion of younger fish. Estimates of total, natural and fishing mortality were greater for M. elongatus than L. argentea across both estuaries, and estimates of total mortality were greatest for both species in Lake Macquarie. The data indicate that neither species has been overfished in these estuaries.

  15. Aging in culture.

    Science.gov (United States)

    Fung, Helene H

    2013-06-01

    This article reviews the empirical studies that test socioemotional aging across cultures. The review focuses on comparisons between Western (mostly North Americans and Germans) and Eastern cultures (mostly Chinese) in areas including age-related personality, social relationships, and cognition. Based on the review, I argue that aging is a meaning-making process. Individuals from each cultural context internalize cultural values with age. These internalized cultural values become goals that guide adult development. When individuals from different cultures each pursue their own goals with age, cultural differences in socioemotional aging occur.

  16. Aging of gaseous detectors

    International Nuclear Information System (INIS)

    Va'Vra, J.

    1990-03-01

    This paper makes an overview of developments in the wire chamber aging field since the wire chamber aging workshop held at the Lawrence Berkeley Laboratory, Berkeley, California on January 16--17, 1986. The author discusses new techniques to analyze the gas impurities and the wire aging products, wire ''nonaging'' in clean systems, wire aging in systems containing various impurities, various examples of problems which can ''prime'' surfaces prior to the occurrence of the aging, and some recent aging experience with the ''SSC micro-straw tubes.'' 35 refs., 10 figs., 2 tabs

  17. Aging and Health: Cataracts

    Science.gov (United States)

    ... Problems Glaucoma Macular Degeneration Join our e-newsletter! Aging & Health A to Z Cataracts Basic Facts & Information ... Are Cataracts? Cataracts are a common result of aging and occur frequently in older people. About one ...

  18. Aging changes in immunity

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/004008.htm Aging changes in immunity To use the sharing features ... cells and antibodies that destroy these harmful substances. AGING CHANGES AND THEIR EFFECTS ON THE IMMUNE SYSTEM ...

  19. National Council on Aging

    Science.gov (United States)

    ... Centers Center for Benefits Access Center for Healthy Aging Donate Skip to Content a A Menu Economic ... Seniors Home Equity Older Workers Money Management Healthy Aging Falls Prevention Chronic Disease Management Senior Hunger & Nutrition ...

  20. NIA Aging Cell Repository

    Data.gov (United States)

    Federal Laboratory Consortium — To facilitate aging research on cells in culture, the NIA provides support for the NIA Aging Cell Repository, located at the Coriell Institute for Medical Research...

  1. Memory and Aging

    Science.gov (United States)

    Memory and Aging Losing keys, misplacing a wallet, or forgetting someone’s name are common experiences. But for people nearing or over age 65, such memory lapses can be frightening. They wonder if they ...

  2. Osteoarticular system aging

    Directory of Open Access Journals (Sweden)

    Edison Rossi

    2008-03-01

    Full Text Available The dichotomy of “natural-aging” versus “disease-aging” in fact doesnot exist. The author discusses the pathophysiology of osteoarticularsystem aging to show facts of bone aging, mainly changings ofarticular cartilage and skeletal muscle tissue aged-related. He stilldiscusses about the skeletal muscle mass defi ciency (sarcopenia inthe frontier of aging (senescence and disease (senility as well asits role in disability and functional dependence that are associated to frailty in many elderly people.

  3. Osteoclasts prefer aged bone

    DEFF Research Database (Denmark)

    Henriksen, K; Leeming, Diana Julie; Byrjalsen, I

    2007-01-01

    We investigated whether the age of the bones endogenously exerts control over the bone resorption ability of the osteoclasts, and found that osteoclasts preferentially develop and resorb bone on aged bone. These findings indicate that the bone matrix itself plays a role in targeted remodeling...... of aged bones....

  4. Physiological Aging and Exercise.

    Science.gov (United States)

    Osness, Wayne

    This paper explores the nature of the aging process by providing an overview of the available evidence relating to the body systems that are most critical to biological function. Each system is treated separately to more clearly describe various aspects of the aging process and then integrated in a discussion of the theories of biological aging.…

  5. Optimal Aging and Death

    DEFF Research Database (Denmark)

    Dalgaard, Carl-Johan Lars; Strulik, Holger

    2010-01-01

    This study introduces physiological aging into a simple model of optimal intertemporal consumption. In this endeavor we draw on the natural science literature on aging. According to the purposed theory, the speed of the aging process and the time of death are endogenously determined by optimal...

  6. Optimal Aging and Death

    DEFF Research Database (Denmark)

    Dalgaard, Carl-Johan; Strulik, Holger

    the representative consumer is subject to physiological aging. In modeling aging we draw on recent research in the fields of biology and medicine. The speed of the aging process, and thus the time of death, are endogenously determined by optimal health investments. We calibrate the model to US data and proceed...

  7. Language and Aging.

    Science.gov (United States)

    Kemper, Susan; Anagnopoulos, Cheryl

    1989-01-01

    Reviews the effects of aging on language usage focusing on three areas of exploration: (1) changes in language in relation to changes in other cognitive abilities, (2) the linguistic consequences of normal aging versus those of dementia and aphasia, and (3) age-group differences in patterns of conversational interaction. (67 references) (GLR)

  8. Age of the universe

    International Nuclear Information System (INIS)

    Parker, B.

    1981-01-01

    The age of the universe is the time that has elapsed since the Big Bang. To calculate the age, the expansion rate of the universe and distance to the galaxies must be determined. Unfortunately, it appears that the expansion rate is not constant but is decelerating. In the 1920's and 30's, Edwin Hubble set out to estimate the age of universe based on the expansion rate and distance to the galaxies. His method is described along with its flaw. Since that time several others have estimated the age of the universe. Their methods as well as results vary. These are discussed in the article. The ages determined from the various methods range from 10 to 20 billion years. There are two independent ways to determine the age of the universe. What they actually do is determine the age of our galaxy which would give a lower limit to the age of the universe. The first method calculates age of globular clusters which yields as age range from 8 to 18 billion years. The second method involves observing the speed at which radioactive substances decay. This also yields and age greater than 10 billion years. It is clear that there is still a lot of work to do before the true age of the universe can be determied

  9. Heart Age PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2015-09-01

    This 60 second public service announcement is based on the September 2015 CDC Vital Signs report. Your heart age is the age of your heart and blood vessels as a result of your risk factors for heart attack and stroke. If you smoke or have high blood pressure, your heart age will be much higher than your actual age. Learn what you can do to lower your heart age and keep it low.  Created: 9/1/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/1/2015.

  10. Computational biology for ageing

    Science.gov (United States)

    Wieser, Daniela; Papatheodorou, Irene; Ziehm, Matthias; Thornton, Janet M.

    2011-01-01

    High-throughput genomic and proteomic technologies have generated a wealth of publicly available data on ageing. Easy access to these data, and their computational analysis, is of great importance in order to pinpoint the causes and effects of ageing. Here, we provide a description of the existing databases and computational tools on ageing that are available for researchers. We also describe the computational approaches to data interpretation in the field of ageing including gene expression, comparative and pathway analyses, and highlight the challenges for future developments. We review recent biological insights gained from applying bioinformatics methods to analyse and interpret ageing data in different organisms, tissues and conditions. PMID:21115530

  11. Aging, longevity and health

    DEFF Research Database (Denmark)

    Rasmussen, Lene Juel; Sander, Miriam; Wewer, Ulla M.

    2011-01-01

    The IARU Congress on Aging, Longevity and Health, held on 5-7 October 2010 in Copenhagen, Denmark, was hosted by Rector Ralf Hemmingsen, University of Copenhagen and Dean Ulla Wewer, Faculty of Health Sciences, University of Copenhagen and was organized by Center for Healthy Aging (CEHA) under......, Muscle and Life Span and Life Span and Mechanisms. Two additional Sessions highlighted ongoing research in the recently established Center for Healthy Aging at the University of Copenhagen. This report highlights outcomes of recent research on aging-related topics, as described at the IARU Congress...... on Aging, Longevity and Health....

  12. Aging and Vision.

    Science.gov (United States)

    Alavi, Marcel V

    2016-01-01

    Aging involves defined genetic, biochemical and cellular pathways that regulate lifespan. These pathways are called longevity pathways and they have relevance for many age-related diseases. In the eye, longevity pathways are involved in the major blinding diseases, cataract, glaucoma, age-related macular degeneration (AMD) and diabetic retinopathy. Pharmaceutical targeting of longevity pathways can extend healthy lifespan in laboratory model systems. This offers the possibility of therapeutic interventions to also delay onset or slow the progression of age-related eye diseases. I suggest that retinal degeneration may be viewed as accelerated aging of photoreceptors and that interventions extending healthy lifespan may also slow the pace of photoreceptor loss.

  13. Ageing Management of CARR

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, H., E-mail: Carr200404@hotmail.com [China Institute of Atomic Energy (CIAE), Beijing (China)

    2014-08-15

    The paper introduced the ageing management of CARR, including the ageing management system, instances of ageing components, difficulties we met and follow up plan, and put forward some suggestions on strengthening and promoting ageing management. We hope to enhance international exchange and collaboration. It is a great challenge to do research on how to manage ageing effectively in the beginning of life, but it will enhance the safety of reactor operation, extend the life of the reactor and improve the quality of operation, making this work very meaningful.

  14. Smoking, health and ageing

    Directory of Open Access Journals (Sweden)

    Nicita-Mauro Claudio

    2008-09-01

    Full Text Available Abstract On March 19, 2008 a Symposium on Pathophysiology of Ageing and Age-Related diseases was held in Palermo, Italy. Here, the lecture of V. Nicita-Mauro on Smoking, health and ageing is summarized. Smoking represents an important ageing accelerator, both directly by triggering an inflammatory responses, and indirectly by favoring the occurrence of several diseases where smoking is a recognized risk factor. Hence, non-smokers can delay the appearance of diseases and of ageing process, so attaining longevity.

  15. Queering Aging Futures

    Directory of Open Access Journals (Sweden)

    Linn J. Sandberg

    2017-08-01

    Full Text Available This paper explores the potential for cultural gerontology to extend its ideas of diversity in aging experiences by opening space to rethink conceptions of successful aging futures. We propose a ‘queering’ of aging futures that disrupts the ways that expectations of a good later life and happy aging are seen to adhere to some bodies and subjectivities over others. Drawing on feminist, queer, and crip theories, we build on existing critiques of ‘successful aging’ to interrogate the assumptions of heteronormativity, able-bodiedness and able-mindedness that shape the dividing lines between success and failure in aging, and which inform attempts to ‘repair’ damaged futures. Conclusions suggest that recognizing diversity in successful aging futures is important in shaping responses to the challenges of aging societies, and presents an opportunity for critical cultural gerontology to join with its theoretical allies in imagining more inclusive alternatives.

  16. Aging and sexuality.

    Science.gov (United States)

    Yee, Lesley

    2010-10-01

    Sexuality has become a medical issue in association with aging. This is due to a number of factors, including increasing age of survival, a positive societal construct that promotes sexuality as important for quality of life as we age, and the medicalisation of sexuality with the advent of prescription medications to treat sexual dysfunction. This article reviews the factors surrounding aging and sexuality and also considers special situations with age, such as institutionalised care and the possibility of elder abuse. Normal physiological changes with aging affect both genders in terms of sexual desire and performance. Other medical conditions increase with age, and these and their treatments will impact on sexuality and the way it can be expressed. Medical practitioners require an understanding of these changes in order to find ways to optimise sexual function in older patients.

  17. Can aging be programmed?

    DEFF Research Database (Denmark)

    Kowald, Axel; Kirkwood, Thomas B. L.

    2016-01-01

    The evolution of the aging process has long been a biological riddle, because it is difficult to explain the evolution of a trait that has apparently no benefit to the individual. Over 60 years ago, Medawar realized that the force of natural selection declines with chronological age because...... of unavoidable environmental risks. This forms the basis of the mainstream view that aging arises as a consequence of a declining selection pressure to maintain the physiological functioning of living beings forever. Over recent years, however, a number of articles have appeared that nevertheless propose...... the existence of specific aging genes; that is, that the aging process is genetically programmed. If this view were correct, it would have serious implications for experiments to understand and postpone aging. Therefore, we studied in detail various specific proposals why aging should be programmed. We find...

  18. The Hallmarks of Aging

    Science.gov (United States)

    López-Otín, Carlos; Blasco, Maria A.; Partridge, Linda; Serrano, Manuel; Kroemer, Guido

    2013-01-01

    Aging is characterized by a progressive loss of physiological integrity, leading to impaired function and increased vulnerability to death. This deterioration is the primary risk factor for major human pathologies including cancer, diabetes, cardiovascular disorders, and neurodegenerative diseases. Aging research has experienced an unprecedented advance over recent years, particularly with the discovery that the rate of aging is controlled, at least to some extent, by genetic pathways and biochemical processes conserved in evolution. This review enumerates nine tentative hallmarks that represent common denominators of aging in different organisms, with special emphasis on mammalian aging. These hallmarks are: genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient-sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. A major challenge is to dissect the interconnectedness between the candidate hallmarks and their relative contribution to aging, with the final goal of identifying pharmaceutical targets to improve human health during aging with minimal side-effects. PMID:23746838

  19. Welfare of Aged Horses

    Directory of Open Access Journals (Sweden)

    Catherine McGowan

    2011-10-01

    Full Text Available Horses form a unique and special part of their owners’ lives and aged horses are no exception. This review considers the health and management of aged horses, including the role of the owner and their perceptions of aged horses, potential threats or risks to their welfare and finally, factors affecting quality of life and euthanasia of aged horses. Owners of aged horses are concerned about the health, welfare and quality of life of their aged animals. Yet surveys of management and preventive healthcare reflect that there may be some limitations to what owners are actually achieving in practice. They show declining management as horses age, particularly for the retired horse and insufficient appropriate preventive healthcare via veterinary surgeons. The veterinary surgeon plays an essential and influential role in preventive healthcare, management of diseases and disorders and ultimately in the decision making process for euthanasia of aged horses at the end of their lives. The value of aged horses should not be underestimated by veterinarians and others working with them and the continuing care of aged horses should be regarded with the same importance as the care of younger horses with more obvious monetary value.

  20. Menopause accelerates biological aging

    Science.gov (United States)

    Levine, Morgan E.; Lu, Ake T.; Chen, Brian H.; Hernandez, Dena G.; Singleton, Andrew B.; Ferrucci, Luigi; Bandinelli, Stefania; Salfati, Elias; Manson, JoAnn E.; Quach, Austin; Kusters, Cynthia D. J.; Kuh, Diana; Wong, Andrew; Teschendorff, Andrew E.; Widschwendter, Martin; Ritz, Beate R.; Absher, Devin; Assimes, Themistocles L.; Horvath, Steve

    2016-01-01

    Although epigenetic processes have been linked to aging and disease in other systems, it is not yet known whether they relate to reproductive aging. Recently, we developed a highly accurate epigenetic biomarker of age (known as the “epigenetic clock”), which is based on DNA methylation levels. Here we carry out an epigenetic clock analysis of blood, saliva, and buccal epithelium using data from four large studies: the Women's Health Initiative (n = 1,864); Invecchiare nel Chianti (n = 200); Parkinson's disease, Environment, and Genes (n = 256); and the United Kingdom Medical Research Council National Survey of Health and Development (n = 790). We find that increased epigenetic age acceleration in blood is significantly associated with earlier menopause (P = 0.00091), bilateral oophorectomy (P = 0.0018), and a longer time since menopause (P = 0.017). Conversely, epigenetic age acceleration in buccal epithelium and saliva do not relate to age at menopause; however, a higher epigenetic age in saliva is exhibited in women who undergo bilateral oophorectomy (P = 0.0079), while a lower epigenetic age in buccal epithelium was found for women who underwent menopausal hormone therapy (P = 0.00078). Using genetic data, we find evidence of coheritability between age at menopause and epigenetic age acceleration in blood. Using Mendelian randomization analysis, we find that two SNPs that are highly associated with age at menopause exhibit a significant association with epigenetic age acceleration. Overall, our Mendelian randomization approach and other lines of evidence suggest that menopause accelerates epigenetic aging of blood, but mechanistic studies will be needed to dissect cause-and-effect relationships further. PMID:27457926

  1. Active ageing technologies

    DEFF Research Database (Denmark)

    Lassen, Aske Juul

    In the recent decade the concept of active aging has become important in the Western hemisphere. The World Health Organization and The European Union have staged active aging as a core policy area and initiated programs of physical activity, independence and prolonged working lives among...... the elderly. As part of this rearticulation of old age, many new technologies take form. This paper uses a wide concept of technologies (devices, regimes, strategies and ways of doing) and argues that technologies form active aging subjectivities, and on the other hand, that these subjectivities...... in their socio-material practices form active aging. Hence, active aging is a mutual entanglement (Callon and Rabeharisoa 2004) between technologies, practices and subjectivities. The paper is based on four months of participant observations and 17 in-depth interviews with elderly persons conducted at three...

  2. The future of ageing.

    Science.gov (United States)

    Hayflick, L

    2000-11-09

    Advances in our knowledge of age-associated diseases have far outpaced advances in our understanding of the fundamental ageing processes that underlie the vulnerability to these pathologies. If we are to increase human life expectancy beyond the fifteen-year limit that would result if today's leading causes of death were resolved, more attention must be paid to basic research on ageing. Determination of longevity must be distinguished from ageing to take us from the common question of why we age to a more revealing question that is rarely posed: why do we live as long as we do? But if the ability to intervene in ageing ever becomes a reality, it will be rife with unintended and undesirable consequences.

  3. The aging lung

    Directory of Open Access Journals (Sweden)

    Lowery EM

    2013-11-01

    Full Text Available Erin M Lowery,1 Aleah L Brubaker,2 Erica Kuhlmann,1 Elizabeth J Kovacs31Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine at Loyola University Medical Center, 2Loyola University Stritch School of Medicine, 3Department of Surgery, Loyola University Medical Center, Maywood, IL, USAAbstract: There are many age-associated changes in the respiratory and pulmonary immune system. These changes include decreases in the volume of the thoracic cavity, reduced lung volumes, and alterations in the muscles that aid respiration. Muscle function on a cellular level in the aging population is less efficient. The elderly population has less pulmonary reserve, and cough strength is decreased in the elderly population due to anatomic changes and muscle atrophy. Clearance of particles from the lung through the mucociliary elevator is decreased and associated with ciliary dysfunction. Many complex changes in immunity with aging contribute to increased susceptibility to infections including a less robust immune response from both the innate and adaptive immune systems. Considering all of these age-related changes to the lungs, pulmonary disease has significant consequences for the aging population. Chronic lower respiratory tract disease is the third leading cause of death in people aged 65 years and older. With a large and growing aging population, it is critical to understand how the body changes with age and how this impacts the entire respiratory system. Understanding the aging process in the lung is necessary in order to provide optimal care to our aging population. This review focuses on the nonpathologic aging process in the lung, including structural changes, changes in muscle function, and pulmonary immunologic function, with special consideration of obstructive lung disease in the elderly.Keywords: aging, lung, pulmonary immunology, COPD

  4. Anatomy of ageing face.

    Science.gov (United States)

    Ilankovan, V

    2014-03-01

    Ageing is a biological process that results from changes at a cellular level, particularly modification of mRNA. The face is affected by the same physiological process and results in skeletal, muscular, and cutaneous ageing; ligamentous attenuation, descent of fat, and ageing of the appendages. I describe these changes on a structural and clinical basis and summarise possible solutions for a rejuvenation surgeon. Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved.

  5. Cave age determination

    International Nuclear Information System (INIS)

    Anon.

    1998-01-01

    To determine the age of a cave we have to determine the age of elements which were created at the same moment when the cave was. Usually a cave is dug out by infiltration of acid waters. During this alteration process rocks free some aluminium and potassium ions. In Carlsbad and Lechuguilla caves, scientists have found alunite, this aluminium and potassium sulfate can be dated by using the carbon method of age determination. (A.C.)

  6. Anorexia of Aging.

    Science.gov (United States)

    Visvanathan, Renuka

    2015-08-01

    The anorexia of aging is common, leading to adverse health consequences. As populations age, the impacts from anorexia in the older population are set to increase. Only greater awareness will allow for prevention or early intervention. This article discusses the physiologic anorexia of aging, highlights contributing factors, and proposes management strategies, including screening, especially in primary care. Many neuroendocrine factors have been implicated in the pathophysiology; it is clear that further human research is necessary if there is to be a pharmacologic breakthrough. There are currently no approved pharmacologic treatment strategies to prevent or treat the anorexia of aging. Copyright © 2015 Elsevier Inc. All rights reserved.

  7. Sexual activity and aging.

    Science.gov (United States)

    Ni Lochlainn, Mary; Kenny, Rose Anne

    2013-08-01

    Sexuality is an important component of emotional and physical intimacy that men and women experience throughout their lives. Research suggesting that a high proportion of men and women remain sexually active well into later life refutes the prevailing myth that aging and sexual dysfunction are inexorably linked. Age-related physiological changes do not render a meaningful sexual relationship impossible or even necessarily difficult. Many of these physiological changes are modifiable. There are various therapeutic options available to patients to achieve maximum sexual capacity in old age. This article reviews the prevalence of sexual activity among older adults, the problems these adults encounter with sexual activity, and the role of the health care professional in addressing these problems. The physiological sex-related changes that occur as part of the normal aging process in men and women are reviewed, as well as the effect of age-related physical and psychological illness on sexual function. The attitudes and perceptions of the media and general public toward sexual activity and aging are summarized. An understanding of the sexual changes that accompany the aging process may help general practitioners and other doctors to give practical and useful advice on sexuality as well as refute the misconception that aging equates to celibacy. A thorough awareness of this aspect of older people's quality of life can raise meaningful expectations for aging patients. Copyright © 2013 American Medical Directors Association, Inc. Published by Elsevier Inc. All rights reserved.

  8. We Are Ageing

    Directory of Open Access Journals (Sweden)

    Genovefa D. Kolovou

    2014-01-01

    Full Text Available Ageing and longevity is unquestioningly complex. Several thoughts and mechanisms of ageing such as pathways involved in oxidative stress, lipid and glucose metabolism, inflammation, DNA damage and repair, growth hormone axis and insulin-like growth factor (GH/IGF, and environmental exposure have been proposed. Also, some theories of ageing were introduced. To date, the most promising leads for longevity are caloric restriction, particularly target of rapamycin (TOR, sirtuins, hexarelin and hormetic responses. This review is an attempt to analyze the mechanisms and theories of ageing and achieving longevity.

  9. Hodgkin's disease and age

    DEFF Research Database (Denmark)

    Specht, L.; Nissen, N.I.

    1989-01-01

    506 unselected, previously untreated patients with Hodgkin's disease were treated at the Finsen Institute between 1969 and 1983. The prognostic significance of age, sex, stage, systemic symptoms, histologic subtype, number of involved nodal regions, total tumour burden (peripheral + intrathoracic...... modality, stage, and total tumour burden, whereas age had no prognostic significance. With regard to death from Hodgkin's disease only age and total tumour burden had independent significance. The significance of age would seem to stem from the fact that some older patients could not be given adequate...

  10. Evolutionary perspectives on ageing.

    Science.gov (United States)

    Reichard, Martin

    2017-10-01

    From an evolutionary perspective, ageing is a decrease in fitness with chronological age - expressed by an increase in mortality risk and/or decline in reproductive success and mediated by deterioration of functional performance. While this makes ageing intuitively paradoxical - detrimental to individual fitness - evolutionary theory offers answers as to why ageing has evolved. In this review, I first briefly examine the classic evolutionary theories of ageing and their empirical tests, and highlight recent findings that have advanced our understanding of the evolution of ageing (condition-dependent survival, positive pleiotropy). I then provide an overview of recent theoretical extensions and modifications that accommodate those new discoveries. I discuss the role of indeterminate (asymptotic) growth for lifetime increases in fecundity and ageing trajectories. I outline alternative views that challenge a universal existence of senescence - namely the lack of a germ-soma distinction and the ability of tissue replacement and retrogression to younger developmental stages in modular organisms. I argue that rejuvenation at the organismal level is plausible, but includes a return to a simple developmental stage. This may exempt a particular genotype from somatic defects but, correspondingly, removes any information acquired during development. A resolution of the question of whether a rejuvenated individual is the same entity is central to the recognition of whether current evolutionary theories of ageing, with their extensions and modifications, can explain the patterns of ageing across the Tree of Life. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Age-based and reproductive biology of the Pacific Longnose Parrotfish Hipposcarus longiceps from Guam

    Directory of Open Access Journals (Sweden)

    Brett M. Taylor

    2017-11-01

    Full Text Available The Pacific longnose parrotfish Hipposcarus longiceps (Valenciennes 1840 represents a prime fishery resource throughout much of the tropical Pacific. In this study, we sampled the species from the Guam commercial fishery market across five consecutive years to characterize reproductive and age-based demographic information imperative for informed fishery management. Compared with other parrotfishes, this species was found to be large-bodied, but has only a moderate life span of 10 + years. Hipposcarus longiceps was confirmed as a diandric protogynous hermaphrodite with highly sex-specific growth patterns and an overall mean asymptotic length of 434 mm fork length (FL. Females were estimated to reach median maturity at 329 mm FL (2.4 years and have a median length at female-to-male sex change of 401 mm FL. Life-history trait values derived here were used to update previous models relating life history and vulnerability to overexploitation. We found that enhancement of just one species’ trait values improved model fits considerably, which strengthens the conclusion that life-history traits are a strong determinant of species’ vulnerability in the parrotfishes. This information is an imperative complement to other data sources facilitating formal stock assessment of a key fishery target.

  12. Cognitive Age: A New Multidimensional Approach to Measuring Age Identity.

    Science.gov (United States)

    Barak, Benny

    1987-01-01

    Conducted exploratory field study to examine how age-concepts are experienced and to assess relationship of age identities to each other. Proposes Cognitive Age as a new multidimensional age scale that merges the standard scale, Identity Age, and Personal Age. Study results attest to Cognitive Age scale's reliability and validity. (Author/NB)

  13. Learning and Active Aging

    Science.gov (United States)

    Boulton-Lewis, Gillian M.; Buys, Laurie; Lovie-Kitchin, Jan

    2006-01-01

    Learning is an important aspect of aging productively. This paper describes results from 2645 respondents (aged from 50 to 74+ years) to a 165-variable postal survey in Australia. The focus is on learning and its relation to work; social, spiritual, and emotional status; health; vision; home; life events; and demographic details. Clustering…

  14. Biology of ageing

    DEFF Research Database (Denmark)

    Rattan, Suresh

    2015-01-01

    Living systems owe their survival and health to a series of complex biochemical pathways of maintenance and repair. These defense systems create the homeodynamic space of an individual, which is characterized by stress tolerance, molecular damage control and continuous remodeling. Ageing, age-rel...

  15. Sexuality and Aging.

    Science.gov (United States)

    Hinkley, Nancy E.

    Literature on sexuality and aging and Maslow's hierarchy of needs (which include physiological needs, safety and security needs, belongingness needs, esteem needs, and the need for self-actualization) are used in this paper to identify at each level the needs of the aging individual as they derive from his sexuality in order to provide a…

  16. Sexuality, aging, and dementia.

    Science.gov (United States)

    Benbow, Susan Mary; Beeston, Derek

    2012-07-01

    Sexuality in later life and its relationship to dementia is a neglected topic: greater understanding of the area has the potential to contribute to the quality of life of people with dementia, their family members, and formal carers. We review current knowledge about sexuality, aging, and dementia. We undertook a review of the recent literature to examine of the following areas: what is known about sexuality and aging, and about attitudes to sexuality and aging; what is known about the relevance of sexuality and aging to people living with dementia and their care; and the management of sexual behaviors causing concern to others. Sexual activity decreases in frequency with increasing age but many older people remain sexually active; there is no age limit to sexual responsiveness; and sexuality is becoming more important to successive cohorts of older people, including people living with dementia and gay, lesbian, bisexual, and transgendered elderly people. Attitudes and beliefs toward sexuality and aging are strongly influenced by stereotypes and myths, not only among the general public but also among those working in health and social care. Professional bodies should include sexuality, aging, and dementia in their training curricula. More work is needed on the impact of environmental issues, particularly in group living situations, on older adults' sexuality, and on consent issues. Ethical decision-making frameworks can be useful in practice. Organizations should investigate how to support staff in avoiding a problem-orientated approach and focus on providing holistic person-centered care.

  17. Age and Scientific Performance.

    Science.gov (United States)

    Cole, Stephen

    1979-01-01

    The long-standing belief that age is negatively associated with scientific productivity and creativity is shown to be based upon incorrect analysis of data. Studies reported in this article suggest that the relationship between age and scientific performance is influenced by the operation of the reward system. (Author)

  18. Healthy ageing at work

    NARCIS (Netherlands)

    de Jong, Marlon; Jolij, Jacob; Lorist, Monicque

    2016-01-01

    Demographic ageing in the Western world means that the average age of the working population is increasing. This has major consequences for the labour process. Growing older is linked to physical and cognitive changes that can influence the performance of tasks. We are faced with an important

  19. Healthy ageing at work

    NARCIS (Netherlands)

    de Jong, Marlon; Jolij, Jacob; Lorist, Monicque

    2016-01-01

    Background Demographic ageing in the Western world means that the average age of the working population is increasing. This has major consequences for the labour process. Growing older is linked to physical and cognitive changes that can influence the performance of tasks. We are faced with an

  20. Brain atrophy during aging

    International Nuclear Information System (INIS)

    Matsuzawa, Taiju; Takeda, Shumpei; Hatazawa, Jun

    1985-01-01

    Age-related brain atrophy was investigated in thousands of persons with no neurologic disturbances using X-CT and NMR-CT and following results were obtained. Brain atrophy was minimal in 34 -- 35 years old in both sexes, increased exponentially to the increasing age after 34 -- 35 years, and probably resulted in dementia, such as vascular or multiinfarct dementia. Brain atrophy was significantly greater in men than in women at all ages. Brain volumes were maximal in 34 -- 35 years old in both sexes with minimal individual differences which increased proportionally to the increasing age. Remarkable individual differences in the extents of brain atrophy (20 -- 30 %) existed among aged subjects. Some aged subjects had little or no atrophy of their brains, as seen in young subjects, and others had markedly shrunken brains associated with senility. From these results there must be pathological factors promoting brain atrophy with a great individual difference. We have studied the relation of intelligence to brain volume, and have ascertained that progression of brain atrophy was closely related to loss of mental activities independently of their ages. Our longitudinal study has revealed that the most important factors promoting brain atrophy during aging was decrease in the cerebral blood flow. MNR-CT can easily detected small infarction (lacunae) and edematous lesions resulting from ischemia and hypertensive encephalopathy, while X-CT can not. Therefore NMR-CT is very useful for detection of subtle changes in the brain. (J.P.N.)

  1. Biodemography of human ageing

    DEFF Research Database (Denmark)

    Vaupel, James W

    2010-01-01

    Human senescence has been delayed by a decade. This finding, documented in 1994 and bolstered since, is a fundamental discovery about the biology of human ageing, and one with profound implications for individuals, society and the economy. Remarkably, the rate of deterioration with age seems...

  2. Successful ageing for psychiatrists.

    Science.gov (United States)

    Peisah, Carmelle

    2016-04-01

    This paper aims to explore the concept and determinants of successful ageing as they apply to psychiatrists as a group, and as they can be applied specifically to individuals. Successful ageing is a heterogeneous, inclusive concept that is subjectively defined. No longer constrained by the notion of "super-ageing", successful ageing can still be achieved in the face of physical and/or mental illness. Accordingly, it remains within the reach of most of us. It can, and should be, person-specific and individually defined, specific to one's bio-psycho-social and occupational circumstances, and importantly, reserves. Successful professional ageing is predicated upon insight into signature strengths, with selection of realistic goal setting and substitution of new goals, given the dynamic nature of these constructs as we age. Other essential elements are generativity and self-care. Given that insight is key, taking a regular stock or inventory of our reserves across bio-psycho-social domains might be helpful. Importantly, for successful ageing, this needs to be suitably matched to the professional task and load. This lends itself to a renewable personal ageing plan, which should be systemically adopted with routine expectations of self-care and professional responsibility. © The Royal Australian and New Zealand College of Psychiatrists 2015.

  3. Aging of SRC liquids

    Science.gov (United States)

    Hara, T.; Jones, L.; Tewari, K. C.; Li, N. C.

    1981-02-01

    The viscosity of SRC-LL liquid increases when subjected to accelerated aging by bubbling oxygen in the presence of copper strip at 62°C. Precipitates are formed and can be separated from the aged liquid by Soxhlet extraction with pentane. A 30-70 blend of SRC-I with SRC-LL was subjected to oxygen aging in the absence of copper, and the viscosity increased dramatically after 6 days at 62°. The content of preasphaltene and its molecular size increase with time of aging, accompanied by decrease of asphaltene and pentane-soluble contents. For the preasphaltene fraction on aging, gel permeation chromatography shows formation of larger particles. ESR experiments show that with oxygen aging, spin concentration in the preasphaltene fraction decreases. Perhaps some semiquinone, together with di- and tri-substituted phenoxy radicals, generated by oxygen aging of the coal liquid, interact with the free radicals already present in coal to yield larger particles and reduce free radical concentration. We are currently using the very high-field (600-MHz) NMR spectrometer at Mellon Institute to determine changes in structural parameters before and after aging of SRC-II and its chromatographically separated fractions.

  4. Healthy ageing at work

    NARCIS (Netherlands)

    de Jong, Marlon; Jolij, Jacob; Lorist, Monicque

    2015-01-01

    Demographic ageing in the Western world means that the average age of the working population is increasing. This has major consequences for the labour process. Growing older is linked to physical and cognitive changes which can influence performance of tasks. We are faced with an important

  5. Age, Wage and Productivity

    NARCIS (Netherlands)

    van Ours, J.C.; Stoeldraijer, L.

    2010-01-01

    Previous empirical studies on the effect of age on productivity and wages find contradicting results. Some studies find that if workers grow older there is an increasing gap between productivity and wages, i.e. wages increase with age while productivity does not or does not increase at the same

  6. Unemployment Dynamics and Age

    NARCIS (Netherlands)

    Berg, van den G.J.; Gijsbert, A.; Lomwel, van C.; Ours, van Jan C.

    1998-01-01

    Youth unemployment is an issue of primary concern in WesternEuropean countries. In this paper we analyze dynamics in unemployment foryouths, adults (prime-aged individuals), and elderly. We use quarterly Frenchunemployment data, stratified by gender, age group, and duration, over theperiod

  7. Optimal Aging and Death

    DEFF Research Database (Denmark)

    Dalgaard, Carl-Johan Lars; Strulik, Holger

    2010-01-01

    health investments. At the same time, physiological aspects of the aging process influence optimal savings and health investment. We calibrate the model for the average US male in 2000 and proceed to show that the calibrated model accounts well for the cross-country link between labor productivity......This study introduces physiological aging into a simple model of optimal intertemporal consumption. In this endeavor we draw on the natural science literature on aging. According to the purposed theory, the speed of the aging process and the time of death are endogenously determined by optimal...... and life expectancy in the same year ("the Preston curve"); cross-country income differences can explain differences in life expectancy at age 20 of up to a decade. Moreover, technological change in health care of about 1.1% per year can account for the observed shift in the Preston curve between 1980...

  8. Modulating aging and longevity

    DEFF Research Database (Denmark)

    Rattan, Suresh

    Provides information and an evaluation of a variety of approaches tried for modulating aging and longevity, including dietary supplementation with antioxidants, vitamins and hormones, genetic engineering, life-style alterations, and hormesis through mild stress. After decades of systematic collec....... The goal of research on ageing is not to increase human longevity regardless of the consequences, but to increase active longevity free from disability and functional dependence......Provides information and an evaluation of a variety of approaches tried for modulating aging and longevity, including dietary supplementation with antioxidants, vitamins and hormones, genetic engineering, life-style alterations, and hormesis through mild stress. After decades of systematic...... collection of data describing age-related changes in organisms, organs, tissues, cells and macromolecules, biogerontologists are now in a position to construct general principles of ageing and explore various possibilities of intervention using rational approaches. While not giving serious consideration...

  9. Parylene C Aging Studies.

    Energy Technology Data Exchange (ETDEWEB)

    Achyuthan, Komandoor; Sawyer, Patricia Sue.; Mata, Guillermo Adrian; White II, Gregory Von; Bernstein, Robert

    2014-09-01

    Parylene C is used in a device because of its conformable deposition and other advantages. Techniques to study Parylene C aging were developed, and "lessons learned" that could be utilized for future studies are the result of this initial study. Differential Scanning Calorimetry yielded temperature ranges for Parylene C aging as well as post-deposition treatment. Post-deposition techniques are suggested to improve Parylene C performance. Sample preparation was critical to aging regimen. Short-term (%7E40 days) aging experiments with free standing and ceramic-supported Parylene C films highlighted "lessons learned" which stressed further investigations in order to refine sample preparation (film thickness, single sided uniform coating, machine versus laser cutting, annealing time, temperature) and testing issues ("necking") for robust accelerated aging of Parylene C.

  10. NAD+ in Aging

    DEFF Research Database (Denmark)

    Fang, Evandro F.; Lautrup, Sofie; Hou, Yujun

    2017-01-01

    The coenzyme NAD+ is critical in cellular bioenergetics and adaptive stress responses. Its depletion has emerged as a fundamental feature of aging that may predispose to a wide range of chronic diseases. Maintenance of NAD+ levels is important for cells with high energy demands and for proficient...... neuronal function. NAD+ depletion is detected in major neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases, cardiovascular disease and muscle atrophy. Emerging evidence suggests that NAD+ decrements occur in various tissues during aging, and that physiological and pharmacological...... interventions bolstering cellular NAD+ levels might retard aspects of aging and forestall some age-related diseases. Here, we discuss aspects of NAD+ biosynthesis, together with putative mechanisms of NAD+ action against aging, including recent preclinical and clinical trials. Recent discoveries have...

  11. Frailty Across Age Groups.

    Science.gov (United States)

    Pérez-Zepeda, M U; Ávila-Funes, J A; Gutiérrez-Robledo, L M; García-Peña, C

    2016-01-01

    The implementation of an aging biomarker into clinical practice is under debate. The Frailty Index is a model of deficit accumulation and has shown to accurately capture frailty in older adults, thus bridging biological with clinical practice. To describe the association of socio-demographic characteristics and the Frailty Index in different age groups (from 20 to over one hundred years) in a representative sample of Mexican subjects. Cross-sectional analysis. Nationwide and population-representative survey. Adults 20-years and older interviewed during the last Mexican National Health and Nutrition Survey (2012). A 30-item Frailty Index following standard construction was developed. Multi-level regression models were performed to test the associations of the Frailty Index with multiple socio-demographic characteristics across age groups. A total of 29,504 subjects was analyzed. The 30-item Frailty Index showed the highest scores in the older age groups, especially in women. No sociodemographic variable was associated with the Frailty Index in all the studied age groups. However, employment, economic income, and smoking status were more consistently found across age groups. To our knowledge, this is the first report describing the Frailty Index in a representative large sample of a Latin American country. Increasing age and gender were closely associated with a higher score.

  12. Probabilistic population aging

    Science.gov (United States)

    2017-01-01

    We merge two methodologies, prospective measures of population aging and probabilistic population forecasts. We compare the speed of change and variability in forecasts of the old age dependency ratio and the prospective old age dependency ratio as well as the same comparison for the median age and the prospective median age. While conventional measures of population aging are computed on the basis of the number of years people have already lived, prospective measures are computed also taking account of the expected number of years they have left to live. Those remaining life expectancies change over time and differ from place to place. We compare the probabilistic distributions of the conventional and prospective measures using examples from China, Germany, Iran, and the United States. The changes over time and the variability of the prospective indicators are smaller than those that are observed in the conventional ones. A wide variety of new results emerge from the combination of methodologies. For example, for Germany, Iran, and the United States the likelihood that the prospective median age of the population in 2098 will be lower than it is today is close to 100 percent. PMID:28636675

  13. Aging in Colombia

    Directory of Open Access Journals (Sweden)

    María Lourdes SANTOS PÉREZ

    2015-12-01

    Full Text Available Using data from statistical sources and official reports, we propose a critical approach to aging in Colombia, specifically from the point of view of public policy in progress or in planning. Our main hypothesis is that these measures refuse comprehensive treatment of the ageing in terms of needs and rights promoting a pure welfare system which, instead, contributes to reinforce a collective imagination of a dependent and impoverished old age, with «fatal» consequences that sustain and reproduce the socioeconomic system.

  14. Assessment of skeletal age

    International Nuclear Information System (INIS)

    Ahn, Hyung Kyu

    1971-01-01

    The author investigated the carpal bone and ephiphyses on the roentgenographic films. Several methods have been used in the study of carpal bones but I have calculated the numbers of ossification center. The subjects consisted of 259 boys and 239 girls from 3 to 13 years of age. The results are summarized as follows; 1. The number of ossification center were increased in aging both boys and girls. 2. Ossification tendency was more faster boys than girls and the difference of left and right hand were non significant . 3. The ossification of ephiphyses was completed in 4 years of age.

  15. Subjective Age in Early Adolescence: Relationships with Chronological Age, Pubertal Timing, Desired Age, and Problem Behaviors

    Science.gov (United States)

    Hubley, Anita M.; Arim, Rubab G.

    2012-01-01

    Subjective age generally refers to the age that one feels. In a cross-sectional questionnaire study of 245 adolescents ages 10-14 years, we examined (a) whether, and when, a cross-over in subjective age occurs, (b) differences in subjective age among pubertal timing groups, (c) correlations between subjective age and each of desired age and five…

  16. Ageing of research reactors

    International Nuclear Information System (INIS)

    Ciocanescu, M.

    2001-01-01

    Historically, many of the research institutions were centred on a research reactor facility as main technological asset and major source of neutrons for research. Important achievements were made in time in these research institutions for development of nuclear materials technology and nuclear safety for nuclear energy. At present, ageing of nuclear research facilities among these research reactors and ageing of staff are considerable factors of reduction of competence in research centres. The safe way of mitigation of this trend deals with ageing management by so called, for power reactors, Plant Life Management and new investments in staff as investments in research, or in future resources of competence. A programmatic approach of ageing of research reactors in correlation with their actual and future utilisation, will be used as a basis for safety evaluation and future spending. (author)

  17. Ageing and skills

    DEFF Research Database (Denmark)

    Desjardins, Richard; Warnke, Arne Jonas

    The relationship between ageing and skills is becoming an important policy issue, not least in the context of population ageing. Data from the Programme for the International Assessment of Adult Competencies (PIAAC) will potentially add considerably to the understanding of the relationship between...... ageing and foundation skills. In particular, the fact that data from the 1994-1998 International Adult Literacy Survey (IALS) and the 2003-2007 Adult Literacy and Lifeskills Survey (ALL) will be linked with PIAAC offers a unique opportunity to examine trends over time at the cohort level for a wide range...... of countries. Specifically, repeated measures will enable an analysis of whether there is skill gain and skill loss over the lifespan of cohorts and overtime between cohorts. This is especially important because age-skill profiles observed on the basis of a single cross-section are difficult to interpret...

  18. Initial brain aging

    DEFF Research Database (Denmark)

    Thomsen, Kirsten; Yokota, Takashi; Hasan-Olive, Md Mahdi

    2018-01-01

    Brain aging is accompanied by declining mitochondrial respiration. We hypothesized that mitochondrial morphology and dynamics would reflect this decline. Using hippocampus and frontal cortex of a segmental progeroid mouse model lacking Cockayne syndrome protein B (CSB(m/m)) and C57Bl/6 (WT......) controls and comparing young (2-5 months) to middle-aged mice (13-14 months), we found that complex I-linked state 3 respiration (CI) was reduced at middle age in CSB(m/m) hippocampus, but not in CSB(m/m) cortex or WT brain. In hippocampus of both genotypes, mitochondrial size heterogeneity increased....... Mitochondrial DNA content was lower, and hypoxia-induced factor 1α mRNA was greater at both ages in CSB(m/m) compared to WT brain. Our findings show that decreased CI and increased mitochondrial size heterogeneity are highly associated and point to declining mitochondrial quality control as an initial event...

  19. Neuromodulators for Aging Skin

    Science.gov (United States)

    ... Light treatment to the skin and Restylane to tear troughs and outer eyebrow. Photo courtesy of Rebecca Fitzagerald, MD - Los Angeles, California Possible risks Soreness Mild bruising Temporary weakness of surrounding muscles Headache Drooping eyelid Why choose neuromodulators for aging ...

  20. Do Bacteria Age?

    Indian Academy of Sciences (India)

    Bacteria are thought to be examples of organisms that do not age. They divide by .... carry genetic material to the next generation through the process of reproduction; they are also .... molecules, and modified proteins. This report revealed that ...