SAFETY AT FLUOR HANFORD (A) CASE STUDY - PREPARED BY THUNDERBIRD SCHOOL OF GLOBAL MANAGEMENT

Energy Technology Data Exchange (ETDEWEB)

ARNOLD LD

2009-09-25

By November of 1997, Fluor Hanford (Fluor) had been the site manager of the Hanford nuclear reservation for a year. The Hanford site had been established as part of the Manhattan Project in the 1940s that gave birth to the atomic bomb. Hanford produced two thirds of U.S. plutonium during the Cold War period. The Hanford site was half the size of Rhode Island and occupied 586 square miles in southeastern Washington State. The production of plutonium for more than 40 years left a huge legacy of chemical and radiological contamination: 80 square miles of contaminated groundwater; 2,300 tons of spent nuclear fuel stored in underwater basins; 20 tons of plutonium-laced contaminated materials; and 500 contaminated facilities. The cleanup involved a challenging combination of radioactive material handling within an infrastructure constructed in the 1940s and 1950s. The cleanup that began in 1988 was expected to take 30 years or more. Improving safety at Hanford had already proven to be a significant challenge. As the new site manager at Hanford, Fluor Hanford inherited lower- and mid-level managers and thousands of unionized employees, many of whom were second or third generation Hanford employees. These employees had seen many contractors come and go over the years. Some of the managers who had worked with the previous contractor saw Fluor's emphasis on safety as getting in the way of operations. Union-management relations were fractious. Hanford's culture was described as 'production driven-management told everyone what to do, and, if you didn't do it, there were consequences'. Worker involvement in designing and implementing safety programs was negligible. Fluor Hanford also was having trouble satisfying its client, the Department of Energy (DOE). The DOE did not see a clear path forward for performance improvements at Hanford. Clearly, major change was necessary, but how and where should it be implemented?

SAFETY AT FLUOR HANFORD (A) CASE STUDY - PREPARED BY THUNDERBIRD SCHOOL OF GLOBAL MANAGEMENT

International Nuclear Information System (INIS)

Arnold, L.D.

2009-01-01

By November of 1997, Fluor Hanford (Fluor) had been the site manager of the Hanford nuclear reservation for a year. The Hanford site had been established as part of the Manhattan Project in the 1940s that gave birth to the atomic bomb. Hanford produced two thirds of U.S. plutonium during the Cold War period. The Hanford site was half the size of Rhode Island and occupied 586 square miles in southeastern Washington State. The production of plutonium for more than 40 years left a huge legacy of chemical and radiological contamination: 80 square miles of contaminated groundwater; 2,300 tons of spent nuclear fuel stored in underwater basins; 20 tons of plutonium-laced contaminated materials; and 500 contaminated facilities. The cleanup involved a challenging combination of radioactive material handling within an infrastructure constructed in the 1940s and 1950s. The cleanup that began in 1988 was expected to take 30 years or more. Improving safety at Hanford had already proven to be a significant challenge. As the new site manager at Hanford, Fluor Hanford inherited lower- and mid-level managers and thousands of unionized employees, many of whom were second or third generation Hanford employees. These employees had seen many contractors come and go over the years. Some of the managers who had worked with the previous contractor saw Fluor's emphasis on safety as getting in the way of operations. Union-management relations were fractious. Hanford's culture was described as 'production driven-management told everyone what to do, and, if you didn't do it, there were consequences'. Worker involvement in designing and implementing safety programs was negligible. Fluor Hanford also was having trouble satisfying its client, the Department of Energy (DOE). The DOE did not see a clear path forward for performance improvements at Hanford. Clearly, major change was necessary, but how and where should it be implemented?

Fluor Hanford Nuclear Material Stabilization Project Welding Manual

International Nuclear Information System (INIS)

BERKEY, J.R.

2000-01-01

The purpose of this section of the welding manual is to: (1) Provide a general description of the major responsibilities of the organizations involved with welding. (2) Provide general guidance concerning the application of codes related to welding. This manual contains requirements for welding for all Fluor Hanford (FH) welding operators working on the W460 Project, in the Plutonium Finishing Plant (PFP) at the U. S. Department of Energy (DOE) Hanford facilities. These procedures and any additional requirements for these joining processes can be used by all FH welding operators that are qualified. The Welding Procedure Specifications (WPS) found in this document were established from Procedure Qualification Records (PQR) qualified by FH specifically for the W460 Project. PQRs are permanent records of the initial testing and qualification program and are used to backup, and support, the WPS. The identification numbers of the supporting PQR(s) are recorded on each WPS. All PQRs are permanently stored under the supervision of the Fluor Hanford Welding Engineer (FHWE). New PQRs and WPSs will continue to be developed as necessary. The qualification of welders, welding operators and welding procedures will be performed for FH under supervision and concurrent of the FHWE. All new welding procedures to be entered in this manual or welder personnel to be added to the welder qualification database, shall be approved by the FHWE

Fluorescence of Alexa fluor dye tracks protein folding.

Directory of Open Access Journals (Sweden)

Simon Lindhoud

Full Text Available Fluorescence spectroscopy is an important tool for the characterization of protein folding. Often, a protein is labeled with appropriate fluorescent donor and acceptor probes and folding-induced changes in Förster Resonance Energy Transfer (FRET are monitored. However, conformational changes of the protein potentially affect fluorescence properties of both probes, thereby profoundly complicating interpretation of FRET data. In this study, we assess the effects protein folding has on fluorescence properties of Alexa Fluor 488 (A488, which is commonly used as FRET donor. Here, A488 is covalently attached to Cys69 of apoflavodoxin from Azotobacter vinelandii. Although coupling of A488 slightly destabilizes apoflavodoxin, the three-state folding of this protein, which involves a molten globule intermediate, is unaffected. Upon folding of apoflavodoxin, fluorescence emission intensity of A488 changes significantly. To illuminate the molecular sources of this alteration, we applied steady state and time-resolved fluorescence techniques. The results obtained show that tryptophans cause folding-induced changes in quenching of Alexa dye. Compared to unfolded protein, static quenching of A488 is increased in the molten globule. Upon populating the native state both static and dynamic quenching of A488 decrease considerably. We show that fluorescence quenching of Alexa Fluor dyes is a sensitive reporter of conformational changes during protein folding.

Magnetic study of solid uranium-fluorine complexes; Contribution a l'etude magnetique de composes fluores solides de l'uranium

Energy Technology Data Exchange (ETDEWEB)

Dianoux, A J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1969-06-01

A study of the magnetic susceptibility of uranium V fluorine complexes and of the magnetic resonance of fluorine atoms in uranium VI fluorine complexes has made it possible to put forward a structural model for these compounds for which it is impossible, because of the lack of suitable single crystals for X-ray diffraction work, to deduce the exact position of the fluorine atoms. It is shown that it is difficult to interpret the paramagnetism of uranium fluorides, because the uranium ions are in low-symmetry sites. A theoretical study of the magnetism of the U{sup V} ion in complex fluorides of the type M{sub 3}UF{sub 8} (M = NH{sub 4}, Na, Rb, Cs) leads to an interpretation based on a trigonal deformation of the eight fluorine atom structure surrounding the uranium atom. By applying a Hamiltonian spin formalism and making a systematic use of group theory, it is possible to present the susceptibility calculations very concisely. Study of the resonance and of the relaxation of the fluorine atoms in powdered uranium VI complex fluorides suggests a structural model in the case of NaUF{sub 7}. It is shown that the shape of the magnetic resonance absorption lines is strongly affected by the presence of large anisotropic chemical shifts. In the model proposed here, six fluorine atoms are linked to the uranium, atom by strongly covalent bonds in a deformed UF{sub 6} octahedral structure; the seventh fluorine atom remains ionic. The occurrence of a rotational movement of the octahedron is confirmed by a study of the longitudinal relaxation of the fluorine atoms, the activation energy being 0.46 eV. (author) [French] L'etude de la susceptibilite magnetique de complexes fluores d'uranium V et la resonance magnetique des fluors dans des complexes fluores d'uranium VI permettent de proposer un modele structural pour ces composes, ou la diffraction des rayons X, en l'absence de monocristaux convenables, est incapable de preciser la position des atomes de fluor. Nous montrons

Tri-State Synfuels Project Review: Volume 12. Fluor project status. [Proposed Henderson, Kentucky coal to gasoline plant; engineering

Energy Technology Data Exchange (ETDEWEB)

1982-06-01

The purpose of this report is to document and summarize activities associated with Fluor's efforts on the Tri-State Synfuels Project. The proposed facility was to be coal-to-transport fuels facility located in Henderson, Kentucky. Tri-State Synfuels Company was participating in the project as a partner of the US Department of Energy per terms of a Cooperative Agreement resulting from DOE's synfuel's program solicitation. Fluor's initial work plan called for preliminary engineering and procurement services to the point of commitment for construction for a Sasol Fischer-Tropsch plant. Work proceeded as planned until October 1981 when results of alternative coal-to-methanol studies revealed the economic disadvantage of the Synthol design for US markets. A number of alternative process studies followed to determine the best process configuration. In January 1982 Tri-State officially announced a change from Synthol to a Methanol to Gasoline (MTG) design basis. Further evaluation and cost estimates for the MTG facility eventually led to the conclusion that, given the depressed economic outlook for alternative fuels development, the project should be terminated. Official announcement of cancellation was made on April 13, 1982. At the time of project cancellation, Fluor had completed significant portions of the preliminary engineering effort. Included in this report are descriptions and summaries of Fluor's work during this project. In addition location of key project data and materials is identified and status reports for each operation are presented.

Magnetic study of solid uranium-fluorine complexes; Contribution a l'etude magnetique de composes fluores solides de l'uranium

Energy Technology Data Exchange (ETDEWEB)

Dianoux, A.J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1969-06-01

A study of the magnetic susceptibility of uranium V fluorine complexes and of the magnetic resonance of fluorine atoms in uranium VI fluorine complexes has made it possible to put forward a structural model for these compounds for which it is impossible, because of the lack of suitable single crystals for X-ray diffraction work, to deduce the exact position of the fluorine atoms. It is shown that it is difficult to interpret the paramagnetism of uranium fluorides, because the uranium ions are in low-symmetry sites. A theoretical study of the magnetism of the U{sup V} ion in complex fluorides of the type M{sub 3}UF{sub 8} (M = NH{sub 4}, Na, Rb, Cs) leads to an interpretation based on a trigonal deformation of the eight fluorine atom structure surrounding the uranium atom. By applying a Hamiltonian spin formalism and making a systematic use of group theory, it is possible to present the susceptibility calculations very concisely. Study of the resonance and of the relaxation of the fluorine atoms in powdered uranium VI complex fluorides suggests a structural model in the case of NaUF{sub 7}. It is shown that the shape of the magnetic resonance absorption lines is strongly affected by the presence of large anisotropic chemical shifts. In the model proposed here, six fluorine atoms are linked to the uranium, atom by strongly covalent bonds in a deformed UF{sub 6} octahedral structure; the seventh fluorine atom remains ionic. The occurrence of a rotational movement of the octahedron is confirmed by a study of the longitudinal relaxation of the fluorine atoms, the activation energy being 0.46 eV. (author) [French] L'etude de la susceptibilite magnetique de complexes fluores d'uranium V et la resonance magnetique des fluors dans des complexes fluores d'uranium VI permettent de proposer un modele structural pour ces composes, ou la diffraction des rayons X, en l'absence de monocristaux convenables, est incapable de preciser la position des atomes de

Environmental Solutions, A Summary of Contributions for FY04: PNNL Contributions to Fluor Hanford

Energy Technology Data Exchange (ETDEWEB)

Fassbender, Linda L.

2005-03-08

Pacific Northwest National Laboratory managed a variety of technical and scientific efforts to support Fluor Hanford's work in cleaning up the Hanford Site. Work done for other Hanford contractors, the Waste Treatment Plant, and directly for the U.S. Department of Energy is summarized in the other booklets in this series.

Comparison of Measurements and FluorMOD Simulations for Solar Induced Chlorophyll Fluorescence and Reflectance of a Corn Crop under Nitrogen Treatments [SIF and Reflectance for Corn

Science.gov (United States)

Middleton, Elizabeth M.; Corp, Lawrence A.; Campbell, Petya K. E.

2007-01-01

The FLuorescence Explorer (FLEX) satellite concept is one of six semifinalist mission proposals selected in 2006 for pre-Phase studies by the European Space Agency (ESA). The FLEX concept proposes to measure passive solar induced chlorophyll fluorescence (SIF) of terrestrial ecosystems. A new spectral vegetation Fluorescence Model (FluorMOD) was developed to include the effects of steady state SIF on canopy reflectance. We used our laboratory and field measurements previously acquired from foliage and canopies of corn (Zea mays L.) under controlled nitrogen (N) fertilization to parameterize and evaluate FluorMOD. Our data included biophysical properties, fluorescence (F) and reflectance spectra for leaves; reflectance spectra of canopies and soil; solar irradiance; plot-level leaf area index; and canopy SIF emissions determined using the Fraunhofer Line Depth principal for the atmospheric telluric oxygen absorption features at 688 nm (O2-beta) and 760 nm (O2-alpha). FluorMOD simulations implemented in the default "look-up-table" mode did not reproduce the observed magnitudes of leaf F, canopy SIF, or canopy reflectance. However, simulations for all of these parameters agreed with observations when the default FluorMOD information was replaced with measurements, although N treatment responses were underestimated. Recommendations were provided to enhance FluorMOD's potential utility in support of SIF field experiments and studies of agriculture and ecosystems.

[Clinical study of the effect of preventing dentine hypersensitiveness by using Fluor Protector and Green Or on the prepared vital pulp abutment teeth].

Science.gov (United States)

Qin, Jia-nan; Lu, Yu-miao; Zhou, Xiao-yan

2005-04-01

To study and evaluate the effect preventing dentine hypersensitiveness by using Fluor Protector or Green Or on the prepared vital pulp abutment teeth of PFM bridges. 118 cases, 246 prepared vital pulp abutment teeth, were randomly divided into three groups: Experimental Group A--treated with the Fluor Protector and temporary crown; Experimental Group B--treated with the Green Or and temporary crown, and Control Group--only using temporary crown. The results of desensitization in 3 groups were evaluated. F test was used for analysis (DSPV6.01). Significant differences were found between experimental Group A, B and the control group after 1 week (when cementing the PFM bridges); and also after 1 month (P0.05). The effect of preventing dental hypersensitiveness by using Fluor Protector or Green Or on the prepared vital pulp abutment teeth of PFM bridges is ideal. It is easy to use and worth being widely applied.

In situ activation of benzyl alcohols with XtalFluor-E: formation of 1,1-diarylmethanes and 1,1,1-triarylmethanes through Friedel-Crafts benzylation.

Science.gov (United States)

Desroches, Justine; Champagne, Pier Alexandre; Benhassine, Yasmine; Paquin, Jean-François

2015-02-28

The Friedel-Crafts benzylation of arenes using benzyl alcohols activated in situ with XtalFluor-E is described. A wide range of 1,1-diarylmethanes and 1,1,1-triarylmethanes were prepared under experimentally simple and mild conditions, without the need for a transition metal or a strong Lewis acid. Notably, the reactivity observed demonstrates the potential of XtalFluor-E to induce C-OH bond ionization and SN1 reactivity of benzylic alcohols.

The effect of monofluorophosphate implant in white rat mothers towards the level of fluor in the incisors of their young babies (Rattus-rattus

Directory of Open Access Journals (Sweden)

Widjijono Widjijono

2010-06-01

Full Text Available Background: Fluoride has been widely used in the prevention of dental caries for a long time. To prevent dental caries, fluoride must be induced in low amount at high frequency. Inducing it through implantation process even make slow release of small concentration of fluoride. Purpose: The aim of this research was to analyze whether the induction of monofluorophosphate (MFP implant into the white rat mothers affects the level of fluoride in the incisors of their young babies. Method: The objects of the research were twenty white rat mothers in two days of pregnancy which then were divided into four groups (n=5. First, those mothers have been induced with implant under their back skin until their born young babies in the age of 35 days (n=5. The level of fluoride in the incisors of those young babies then is measured with Potentiometer. The obtained data were finally analyzed with One-Way ANOVA test and continued by with LSD test (p=0.05. Result: The result of this research showed that the means of the fluoride level in the incisors of those babies divided into those four groups in series were about 11956.16±201.35 ppb (K, 27328.04±234.56 ppb (P1, 37267.21±248.86 ppb (P2, and 18103.50±267.11 ppb (P3. The result of ANOVA test then showed that the induction of various MFP implant levels significantly affected the level of fluoride in the incisors of the babies. The mean differences among the treatment groups after being tested with LSD 0.05 were also significant. Conclusion: The finding confirm that the significant increasing of the optimal fluoride retention in the incisors of white rat babies can be achieved with the induction of fluoride with MFP ions implant in about 52.98 mg.Latar belakang: Pencegahan karies gigi menggunakan senyawa fluor telah banyak dilakukan dan berlangsung dalam jangka waktu lama. Pemberian fluor dalam jumlah rendah dan frekuensi tinggi merupakan pemenuhan kebutuhan pencegahan karies gigi. Pemberian dengan cara

Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments

Energy Technology Data Exchange (ETDEWEB)

Grate, Jay W.; Mo, Kai-For; Shin, Yongsoon; Vasdekis, Andreas; Warner, Marvin G.; Kelly, Ryan T.; Orr, Galya; Hu, Dehong; Dehoff, Karl J.; Brockman, Fred J.; Wilkins, Michael J.

2015-03-18

Cellulose nanocrystal materials have been labeled with modern Alexa Fluor dyes in a process that first links the dye to a cyanuric chloride molecule. Subsequent reaction with cellulose nanocrystals provides dyed solid microcrystalline cellulose material that can be used for bioimaging and suitable for deposition in films and spatially structured microenvironments. It is demonstrated with single molecular fluorescence microscopy that these films are subject to hydrolysis by cellulose enzymes.

Polyurethane/fluor-hydroxyapatite nanocomposite scaffolds for bone tissue engineering. Part I: morphological, physical, and mechanical characterization

Science.gov (United States)

Asefnejad, Azadeh; Behnamghader, Aliasghar; Khorasani, Mohammad Taghi; Farsadzadeh, Babak

2011-01-01

In this study, new nano-fluor-hydroxyapatite (nFHA)/polyurethane composite scaffolds were fabricated for potential use in bone tissue engineering. Polyester urethane samples were synthesized from polycaprolactone, hexamethylene diisocyanate, and 1,4-butanediol as chain extender. Nano fluor-hydroxyapatite (nFHA) was successfully synthesized by sol-gel method. The solid–liquid phase separation and solvent sublimation methods were used for preparation of the porous composites. Mechanical properties, chemical structure, and morphological characteristics of the samples were investigated by compressive test, Fourier transform infrared, and scanning electron microscopy (SEM) techniques, respectively. The effect of nFHA powder content on porosity and pore morphology was investigated. SEM images demonstrated that the scaffolds were constituted of interconnected and homogeneously distributed pores. The pore size of the scaffolds was in the range 50–250 μm. The result obtained in this research revealed that the porosity and pore average size decreased and compressive modulus increased with nFHA percentage. Considering morphological, physical, and mechanical properties, the scaffold with a higher ratio of nFHA has suitable potential use in tissue regeneration. PMID:21289986

Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles

Science.gov (United States)

Wang, Wei; Nallathamby, Prakash D.; Foster, Carmen M.; Morrell-Falvey, Jennifer L.; Mortensen, Ninell P.; Doktycz, Mitchel J.; Gu, Baohua; Retterer, Scott T.

2013-10-01

A new synthesis approach is described that allows the direct incorporation of fluorescent labels into the volume or body of SiO2 nanoparticles. In this process, fluorescent Alexa Fluor dyes with different emission wavelengths were covalently incorporated into the SiO2 nanoparticles during their formation by the hydrolysis of tetraethoxysilane. The dye molecules were homogeneously distributed throughout the SiO2 nanoparticles. The quantum yields of the Alexa Fluor volume-labeled SiO2 nanoparticles were much higher than nanoparticles labeled using conventional organic dyes. The size of the resulting nanoparticles was controlled using microemulsion reaction media with sizes in the range of 20-100 nm and a polydispersity of cultured macrophages. Differences in particle agglomeration and cell association were clearly associated with differences in observed nanoparticle toxicity. The capacity to maintain particle fluorescence while making significant changes to surface chemistry makes these particles extremely versatile and useful for studies of particle agglomeration, uptake, and transport in environmental and biological systems.A new synthesis approach is described that allows the direct incorporation of fluorescent labels into the volume or body of SiO2 nanoparticles. In this process, fluorescent Alexa Fluor dyes with different emission wavelengths were covalently incorporated into the SiO2 nanoparticles during their formation by the hydrolysis of tetraethoxysilane. The dye molecules were homogeneously distributed throughout the SiO2 nanoparticles. The quantum yields of the Alexa Fluor volume-labeled SiO2 nanoparticles were much higher than nanoparticles labeled using conventional organic dyes. The size of the resulting nanoparticles was controlled using microemulsion reaction media with sizes in the range of 20-100 nm and a polydispersity of cultured macrophages. Differences in particle agglomeration and cell association were clearly associated with differences in

FluorWPS: A Monte Carlo ray-tracing model to compute sun-induced chlorophyll fluorescence of three-dimensional canopy

Science.gov (United States)

A model to simulate radiative transfer (RT) of sun-induced chlorophyll fluorescence (SIF) of three-dimensional (3-D) canopy, FluorWPS, was proposed and evaluated. The inclusion of fluorescence excitation was implemented with the ‘weight reduction’ and ‘photon spread’ concepts based on Monte Carlo ra...

Measurement of 2-5 keV x-ray emission from laser-target interactions by using fluor-MCP and CsI-XRD detectors

International Nuclear Information System (INIS)

Lee, P.H.Y.; Tirsell, K.G.; Leipelt, G.R.; Laird, W.B.

1981-01-01

For inertial confinement fusion plasma diagnostics, x-ray diode (XRD) detectors using conventional cathodes are not sensitive enough to measure x-rays above approx. 1.5 keV. However, for laser driver fusion targets, x-rays in the range of 2 to 5 keV are important because of their mobility in the target. We have successfully used fluor-microchannel plate (MCP) detectors to obtain absolute x-ray measurements in the 2 to 5 keV range. Recent data obtained from experiments on the Shiva laser system are presented. In addition, designs for a variety of channels in the range using fluor-MCP and CsI-XRD's above 1.5 keV will be discussed

Adsorption of volatile organic compounds by polytetra-fluor ethylene

International Nuclear Information System (INIS)

Martinet, J.M.

1958-01-01

The sorption of organic vapours by microporous polytetra-fluor ethylene has been studied gravimetrically using a Mc Bain-Baker type sorption balance. The amount of sorption, the peculiarities observed on the isotherm curves, the small influence of temperature, and smallness of hysteresis suggests that mainly physical adsorption occurs when the temperature is around 25 deg. C. The values of the surface areas obtained from the adsorption isotherms using organic vapours differ greatly from those derived from N 2 adsorption measurements. This discrepancy cannot be completely attributed to differences in the structure and chemical function of the adsorbate molecules, or to the porous structure of the adsorbent. On the contrary, the surface area values obtained by sorbing high volatile freons conform with those measured by nitrogen adsorption, which seems to imply a connection between the area of sorbed monolayers and volatility of the adsorbate. (author) [fr

Thermal expansion behavior of fluor-chlorapatite crystalline solutions

Science.gov (United States)

Hovis, G.; Harlov, D.; Gottschalk, M.; Hudacek, W.; Wildermuth, S.

2009-04-01

the fluor-chlorapatite series is little affected by composition. This contrasts with relationships in alkali feldspars (Hovis and coworkers, 1997, 1999), which show that K-rich feldspars expand less than Na-rich feldspars. It contrasts also with the behavior of additional AlSi3 feldspars (Hovis and others, 2008), in which room-temperature chemical expansion limits the degree to which the structure can expand thermally. It also differs from expansion in kalsilite crystalline solutions (Hovis and coworkers, 2003, 2006), which depends on K:Na ratio. Among the minerals we have studied previously, only nepheline displays expansion behavior similar to that of fluor-chlorapatite crystalline solutions in that thermal expansion shows little sensitivity to composition. In AlSi3 feldspars and kalsilite one observes a single crystallographically distinct alkali site and a dominating SiO4 tetrahedral framework that limits the vibrational characteristics of the alkali-site occupant(s). Fluor-chlorapatite crystalline solutions have no such structural framework. Moreover, the anion site in the latter changes structural character in the transition from fluorapatite to chlorapatite. This flexibility apparently allows anion vibrational characteristics, coupled with those of Ca polyhedral components, to change continuously and in a compensating manner across the series. The thermal expansion data also imply that volumes of F-Cl mixing in fluor-chlorapatite are constant from room temperature to 1000 °C. References: Cherniak, D.J. (2000) Rare earth element diffusion in apatite. Geochimica et Cosmochimica Acta 64, 3871-3885. Harlov, D.E. and Förster, H-J. (2002) High grade fluid metasomatism on both a local and regional Scale: the Seward Peninsula, Alaska and the Ivrea-Verbano Zone, Northern Italy Part II: phosphate mineral chemistry. Journal of Petrology 43, 801-824. Holland, T.J.B. and Redfern, S.A.T. (1997) Unit-cell refinement: Changing the dependent variable, and use of regression

Fluor Daniel Hanford Inc. integrated safety management system phase 1 verification final report

International Nuclear Information System (INIS)

PARSONS, J.E.

1999-01-01

The purpose of this review is to verify the adequacy of documentation as submitted to the Approval Authority by Fluor Daniel Hanford, Inc. (FDH). This review is not only a review of the Integrated Safety Management System (ISMS) System Description documentation, but is also a review of the procedures, policies, and manuals of practice used to implement safety management in an environment of organizational restructuring. The FDH ISMS should support the Hanford Strategic Plan (DOE-RL 1996) to safely clean up and manage the site's legacy waste; deploy science and technology while incorporating the ISMS theme to ''Do work safely''; and protect human health and the environment

Anisotropy of the Chemical Shift Tensor for Fluorines in UF{sub 6} : Application to the Fluorine Atom Movement Model; Anisotropie du Tenseur de Deplacement Chimique des Fluors dans UF{sub 6}: Application au Modele du Mouvement des Atomes de Fluor; Anizotropiya tenzora khimicheskogo zameshcheniya ftora v UF{sub 6}. Primenenie k modeli dvizheniya atomov ftora; Anisotropia del Tensor de Desplazamiento Quimico de los Atomos de Fluor en el UF{sub 6}: Aplicacion al Modelo del Movimiento de los Atomos de Fluor

Energy Technology Data Exchange (ETDEWEB)

Rigny, P. [Centre d' Etudes Nucleaires de Saclay (France)

1965-10-15

'uranium poly- cristallin par la resonance magnetique du fluor a ete faite par R. Blinc et coll. a 40 Mc/s. L'auteur rappelle que le spectre obtenu a basse temperature (t < -20 Degree-Sign C) peut s'analyser en une superposition de deux raies, ce qui confirme la structure trouvee par Hoard et Stroupe aux rayons X ou, l'octaedre UF{sub 6} ayant un axe long et deux axes courts, les atomes de fluor se repartissent entre deux types de sites. L'evolution du spectre avec la temperature - coalescence des deux raies - suggere l'existence d'un mouvement des atomes de fluor entre les deux types de sites. En reprenant ces experiences a 56,4 Mc/s et a 94 Mc/s, l'auteur a pu montrer l'existence d'une forte anisotropie axiale du tenseur de deplacement chimique (environ 650 ppm). La raie d'absorption obtenue dans ces conditions pour une poudre est complexe et on est conduit pour l'etudier a envisager une fonction de forme f(h), qui est la probabilite que possede un grain de poudre d'avoir une orientation qui le fait resonner pour la valeur h du champ. En l'absence de mouvement (spectre de basse temperature), la fonction de forme pour chacune des deux raies (correspondant aux deux types de sites) a la forme obtenue pour des atomes tous equivalents. On sait que les parametres des tenseurs de deplacements chimiques renseignent sur le caractere des liaisons chimiques. On est ainsi conduit, par exemple, a attribuer un important caractere ionique (I Asymptotically-Equal-To 1/2) aux liaisons de l'uranium avec les deux atomes de fluor les plus eloignes. En presence de mouvement, la fonction de forme est tres differente et depend du type de mouvement. Pour UF{sub 6}, l'etude de la forme de la raie unique (t > 20 Degree-Sign C) en presence d'anisotropie montre que les atomes de fluor d'une meme molecule s'echangent entre eux, chaque atome passant environ 5 {mu}s a 30 Degree-Sign C dans chacune des positions, avec une energie d'activation d'environ 0,5 eV. (author) [Spanish] R. Blinc y colabs. han

Study of the dissolution of three synthetic minerals: zirconolite, Y-britholite and mono-silicate fluor-apatite; Etude de la solubilite de trois mineraux synthetiques: la zirconolite, la britholite-Y et la fluorapatite monosilicatee

Energy Technology Data Exchange (ETDEWEB)

Kamel, N.E.H.; Telmoune, S.A. [Centre de Recherche Nucleaire d' Alger, Div. des Techniques Nucleaires, Alger (Algeria); Ait-Amar, H. [Unitersite des Sciences et de la Technologie Houari Boumediene, Dept. de Genie des Procedes, Alger (Algeria)

2007-11-15

In this study, we were interested in the synthesis by natural sintering of three minerals analogue of natural rocks: zirconolite, Y-britholite and mono-silicated fluor-apatite. Cerium was used as an actinide surrogate. A simple physical characterization of the materials was made by X-ray diffraction and by measuring both densities and hardnesses. A static leaching test allowed determining the cerium immobilization capacity of the minerals. The most stable mineral was mono-silicated fluor-apatite, with a maximum amount of released cerium less than 2 %. For zirconolite and Y-britholite, this amount reached 15 and 18 % of the total cerium in the minerals, respectively. For the latter compounds, the cerium content in the materials was too weak, and the chosen synthesis method gave less satisfactory physicochemical mineral properties compared to those obtained for mono-silicated fluor-apatite. (authors)

Fluor Daniel Hanford Inc. integrated safety management system phase 1 verification final report

Energy Technology Data Exchange (ETDEWEB)

PARSONS, J.E.

1999-10-28

The purpose of this review is to verify the adequacy of documentation as submitted to the Approval Authority by Fluor Daniel Hanford, Inc. (FDH). This review is not only a review of the Integrated Safety Management System (ISMS) System Description documentation, but is also a review of the procedures, policies, and manuals of practice used to implement safety management in an environment of organizational restructuring. The FDH ISMS should support the Hanford Strategic Plan (DOE-RL 1996) to safely clean up and manage the site's legacy waste; deploy science and technology while incorporating the ISMS theme to ''Do work safely''; and protect human health and the environment.

The isotope-dilution method of fluorine microestimation; Microdosage du fluor pur dilution isotopique; Mikroopredelenie ftora no metodu razbavleniya izotopov; Microvaloracion del fluor por dilucion isotopica

Energy Technology Data Exchange (ETDEWEB)

Kudahl, J N; Fremlin, J H; Hardwick, J L [Department of Physics, University of Birmingham (United Kingdom)

1962-01-15

Fluoride ions are adsorbed on glass from acid solution and, for solutions containing less than 100 ppm of fluorine, the amount adsorbed varies very regularly with concentration. A certain volume of a carrier-free solution of the unstable isotope F{sup 18} is added to each of a set of acid fluoride solutions which may be a few milligrams, or less, in size. If the solutions are applied to a defined area of glass, some of the F{sup 18} is adsorbed and this can be fixed by raising the pH when equilibrium has been reached. The radioactivity of the glass is a measure of the total amount of fluoride on the glass and can be used to draw an adsorption isotherm from which the fluorine content of an unknown solution in the set can be found. The effects of pH, and the presence of calcium and phosphate ions have been studied, and it has been shown that organic materials may be ashed safely if calcium or magnesium ions are present. The accuracy of the method is independent of the fluoride concentration down to concentrations of a fraction of a ppm. (author) [French] L'ion de fluorure d'une solution acide est adsorbe sur du verre; pour des solutions contenant moins de 100 ppm de fluor, la quantite adsorbee varie tres regulierement en fonction de la concentration. Un certain volume d'une solution, sans entraineur-, de l'isotope instable {sup 18}F est ajoute a une serie de solutions acides de fluorure; il peut s'agir de quantites de l'ordre de quelques milligrammes, ou meme moins. Si l'on applique les solutions sur une surface bien definie de verre, une partie du {sup 18}F est adsorbee; on peut la fixer en augmentant le pH des que l'equilibre a ete realise. La radioactivite du verre donne la mesure de la quantite totale de fluorure fixe sur le verre; elle peut servir a etablir l'isotherme d'adsorption, dont en peut deduire la teneur en fluor de toute solution de la serie. Les auteurs ont etudie les effets du pH et la presence d'ions de calcium et de magnesium; ils ont montre que

The occurrence of fluor-wagnerite in UHT granulites and its implications towards understanding fluid regimes in the evolution of deep crust: a case study from the Eastern Ghats Belt, India

Science.gov (United States)

Das, Kaushik; Tomioka, Naotaka; Bose, Sankar; Ando, Jun-ichi; Ohnishi, Ichiro

2017-06-01

We report the occurrence of a rare phosphate mineral, fluor-wagnerite (Mg1.91-1.94Fe0.06-0.07Ca1000 °C, 8-9 kbar) of the studied area was retrogressed after emplacement to mid-crustal level (800-850 °C, 6-6.5 kbar) as deduced from their pressure -temperature histories. Based on mineral chemical data and micro-Raman analyses, we document an unusual high Mg-F-rich chemistry of the F-wagnerite, which occur both in peak metamorphic porphyroblastic assemblages as well as in the retrograde matrix assemblage. Therefore, in absence of other common phosphates like apatite, fluor-wagnerite can act as an indicator for the presence of F-bearing fluids for rocks with high X Mg and/or fO2. The occurrence of F-rich minerals as monitors for fluid compositions has important implications for the onset of biotite dehydration melting and hence melt production in the deep crust. We propose that fluor-wagnerite can occur as an accessory mineral associated with F-rich fluids in lower-mid crustal rocks, and F in coexisting minerals should be taken into consideration when reconciling the petrogenetic grid of biotite-dehydration melting.

Fluor Hanford Integrated Safety Management System Phase II Verification Vol 1 & Vol 2

Energy Technology Data Exchange (ETDEWEB)

PARSONS, J.E.

2000-07-15

The U.S. Department of Energy (DOE) is committed to conducting work efficiently and in a manner that ensures protection of the workers, public, and environment. DOE policy mandates that safety management systems be used to systematically integrate safety into management and work practices at all levels while accomplishing mission goals in an effective and efficient manner. The purpose of the Fluor Hanford (FH) Integrated Safety Management System (ISMS) verification was to determine whether FH's ISM system and processes are sufficiently implemented to accomplish the goal of ''Do work safely.'' The purpose of the DOE, Richland Operations Office (RL) verification was to determine whether RL has established processes that adequately describe RL's role in safety management and if those processes are sufficiently implemented.

Fluor Hanford Integrated Safety Management System Phase II Verification Vol 1 and Vol 2

CERN Document Server

Parsons, J E

2000-01-01

The U.S. Department of Energy (DOE) is committed to conducting work efficiently and in a manner that ensures protection of the workers, public, and environment. DOE policy mandates that safety management systems be used to systematically integrate safety into management and work practices at all levels while accomplishing mission goals in an effective and efficient manner. The purpose of the Fluor Hanford (FH) Integrated Safety Management System (ISMS) verification was to determine whether FH's ISM system and processes are sufficiently implemented to accomplish the goal of ''Do work safely.'' The purpose of the DOE, Richland Operations Office (RL) verification was to determine whether RL has established processes that adequately describe RL's role in safety management and if those processes are sufficiently implemented.

Fluor Hanford Integrated Safety Management System Phase II Verification Vol 1 and Vol 2

International Nuclear Information System (INIS)

PARSONS, J.E.

2000-01-01

The U.S. Department of Energy (DOE) is committed to conducting work efficiently and in a manner that ensures protection of the workers, public, and environment. DOE policy mandates that safety management systems be used to systematically integrate safety into management and work practices at all levels while accomplishing mission goals in an effective and efficient manner. The purpose of the Fluor Hanford (FH) Integrated Safety Management System (ISMS) verification was to determine whether FH's ISM system and processes are sufficiently implemented to accomplish the goal of ''Do work safely.'' The purpose of the DOE, Richland Operations Office (RL) verification was to determine whether RL has established processes that adequately describe RL's role in safety management and if those processes are sufficiently implemented

A novel and selective fluoride opening of aziridines by XtalFluor-E. synthesis of fluorinated diamino acid derivatives.

Science.gov (United States)

Nonn, Melinda; Kiss, Loránd; Haukka, Matti; Fustero, Santos; Fülöp, Ferenc

2015-03-06

The selective introduction of fluorine onto the skeleton of an aminocyclopentane or cyclohexane carboxylate has been developed through a novel and efficient fluoride opening of an activated aziridine ring with XtalFluor-E. The reaction proceeded through a stereoselective aziridination of the olefinic bond of a bicyclic lactam and regioselective aziridine ring opening with difluorosulfiliminium tetrafluoroborate with the neighboring group assistance of the sulfonamide moiety to yield fluorinated diamino acid derivatives. The method based on the selective aziridine opening by fluoride has been generalized to afford access to mono- or bicyclic fluorinated substances.

Dehydration and Rehydration of Carbonated Fluor- and Hydroxylapatite

Directory of Open Access Journals (Sweden)

Claude Yoder

2012-03-01

Full Text Available The recent definitive deuterium solid state NMR spectroscopic evidence for structural water in fluor- and hydroxylapatites has prompted our study of the conditions necessary for the removal and reincorporation of this important structural feature of apatites. Thermal gravimetric analysis of 20 synthetic carbonated calcium hydroxylapatite (CCaApOH samples and nine carbonated calcium fluorapatite (CCaApF samples has been used to determine the amount of structural and adsorbed water in each sample. No correlation between the weight percent and number of moles of structural water and the weight percent carbonate in CCaApOH and CCaApF has been found. In contrast, there appears to be a relationship between the amount of adsorbed water and the carbonate concentration in the fluorapatites prepared with a two hour digestion time, as well as in the hydroxylapatites prepared with one hour digestion periods, presumably due to the effect of carbonate on crystallite size. Structural water can be removed from the apatite lattice, primarily above 200 °C, but heating to over 550 °C is required for complete removal. This water can be partly reincorporated through an apparently kinetically-controlled process that is enhanced by an increase in time and/or temperature. We speculate that the incorporation of structural water occurs at the beginning of the formation of the apatite structure, approximately coincident with the incorporation of carbonate. We also speculate that water is both removed and reincorporated by proton transfers from water molecules to hydroxide ions.

HUBUNGAN KADAR FLUOR AIR MINUM TERHADAP KARIES GIGI PADA ANAK SEKOLAH DASAR DI KECAMATAN LANDONO KABUPATEN KONAWE SELATAN PROVINSI SULAWESI TENGGARA

OpenAIRE

Sunubi, Erni

2013-01-01

JURNAL MASYARAKAT EPIDEMIOLOGI INDONESIA ABSTRACT The aim of the study was to discover the correlation between potable water fluor and dental caries of elementary school children in Landono district, South Konawe regency, Southeast Sulawesi province. The study was cross sectional. The number of samples was 144 six graders elementary school children aged 12 years selected by proportional stratified random sampling. The water samples were collected from water resources consumed by the ch...

Spectrographic determination of chlorine and fluorine; Dosage du chlore et du fluor par spectrographie d'emission en atmosphere inerte

Energy Technology Data Exchange (ETDEWEB)

Contamin, G [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

1965-04-01

Experimental conditions have been investigated in order to obtain the highest sensitivity in spectrographic determination of chlorine and fluorine using the Fassel method of excitation in an inert atmosphere. The influence of the nature of the atmosphere, of the discharge conditions and of the matrix material has been investigated. The following results have been established: 1. chlorine determination is definitely possible: a working curve has been drawn between 10 {mu}g and 100 {mu}g, the detection limit being around 5 {mu}g; 2. fluorine determination is not satisfactory: the detection limit is still of the order of 80 {mu}g. The best operating conditions have been defined for both elements. (author) [French] Nous avons recherche quelles etaient les conditions permettant d'obtenir la meilleure sensibilite dans le dosage spectrographique du chlore et du fluor par la methode d'excitation en atmosphere inerte (methode de Fassel). Nous avons etudie l'influence de l'atmosphere gazeuse, des conditions de la decharge et du materiau de pastillage. Les points suivants ont ete etablis: 1. le dosage du chlore est possible: une courbe de dosage a ete tracee entre 10 {mu}g et 100 {mu}g et la limite de detection est de l'ordre de 5 {mu}g; 2. le dosage du fluor n'est pas satisfaisant: la limite de detection obtenue etant encore de l'ordre de 80 {mu}g. Les conditions operatoires ont ete precisees pour ces deux elements. (auteur)

Efektivitas Desensitizing Agent dengan dan tanpa Fluor pada Metode in Office Bleaching terhadap Kandungan Mineral Gigi (Kajian In Vitro

Directory of Open Access Journals (Sweden)

Yulita Kristanti

2015-12-01

Full Text Available In office bleaching menggunakan hidrogen peroksida 40% sering memberikan efek samping berupa linu baik selama maupun setelah perawatan tersebut dilakukan. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh waktu aplikasi dan kandungan fluor desensitizing agent pada metode in office bleaching terhadap kandungan mineral gigi. Delapan gigi masing-masing dipotong menjadi 4 bagian, 6 potong digunakan untuk pemeriksaan XRD awal. Gigi dikeringkan dalam oven suhu 50° selama 30 menit diserbuk, diambil 1 mg untuk diperiksa kandungan mineralnya dengan goniometer. Dua puluh empat potong yang lain dibagi dalam 4 kelompok perlakuan. Kelompok I: gigi diaplikasi bahan bleaching 0,5-1 mm hidrogen peroksida 40% selama 1 jam, dicuci, dikeringkan, diikuti aplikasi 0,1 ml desensitizing agent tanpa fluor (CPP-ACP selama 30 menit, dicuci, dikeringkan. Kelompok II : gigi diaplikasi CPP-ACP 30 menit, dicuci, dikeringkan, dibleaching menggunakan hidrogen peroksida 40% selama 1 jam. Selanjutnya gigi diaplikasi CPP-ACP 30 menit lagi, dicuci, dikeringkan. Kelompok III gigi dibleaching menggunakan hidrogen peroksida 40% selama 1 jam, dicuci, dikeringkan, diikuti aplikasi desensitizing agent yang mengandung fluor (CPP-ACFP selama 30 menit, dicuci, dikeringkan. Kelompok IV: gigi diaplikasi CPP-ACFP 30 menit, dicuci, dikeringkan, dibleaching menggunakan hidrogen peroksida 40% selama 1 jam. Selanjutnya gigi diaplikasi CPP-ACFP selama 30 menit, dicuci dikeringkan. Sesudah perlakuan, semua gigi dilakukan pemeriksaan kandungan mineral gigi dengan prosedur yang sama. Uji Mann Whitney menunjukkan penurunan mineral paling sedikit terjadi pada kelompok IV (4500. Desensitizing agent mengandung F sebelum dan sesudah perlakuan in office bleaching menunjukkan penurunan mineral paling kecil.   The Effectiveness of Desensitizing Agent with and without Fluorine in Office Bleaching Method to Tooth Mineral content. Tooth sensitivity arises during or after an in

Spectrographic determination of chlorine and fluorine; Dosage du chlore et du fluor par spectrographie d'emission en atmosphere inerte

Energy Technology Data Exchange (ETDEWEB)

Contamin, G. [Commissariat a l' Energie Atomique, Grenoble (France). Centre d' Etudes Nucleaires

1965-04-01

Experimental conditions have been investigated in order to obtain the highest sensitivity in spectrographic determination of chlorine and fluorine using the Fassel method of excitation in an inert atmosphere. The influence of the nature of the atmosphere, of the discharge conditions and of the matrix material has been investigated. The following results have been established: 1. chlorine determination is definitely possible: a working curve has been drawn between 10 {mu}g and 100 {mu}g, the detection limit being around 5 {mu}g; 2. fluorine determination is not satisfactory: the detection limit is still of the order of 80 {mu}g. The best operating conditions have been defined for both elements. (author) [French] Nous avons recherche quelles etaient les conditions permettant d'obtenir la meilleure sensibilite dans le dosage spectrographique du chlore et du fluor par la methode d'excitation en atmosphere inerte (methode de Fassel). Nous avons etudie l'influence de l'atmosphere gazeuse, des conditions de la decharge et du materiau de pastillage. Les points suivants ont ete etablis: 1. le dosage du chlore est possible: une courbe de dosage a ete tracee entre 10 {mu}g et 100 {mu}g et la limite de detection est de l'ordre de 5 {mu}g; 2. le dosage du fluor n'est pas satisfaisant: la limite de detection obtenue etant encore de l'ordre de 80 {mu}g. Les conditions operatoires ont ete precisees pour ces deux elements. (auteur)

Thermal and flow analysis of the Fluor Daniel, Inc., Nuclear Material Storage Facility renovation design (initial 30% effort of Title 1)

International Nuclear Information System (INIS)

Steinke, R.G.; Mueller, C.; Knight, T.D.

1998-03-01

The computational fluid dynamics code CFX4.2 was used to evaluate steady-state thermal-hydraulic conditions in the Fluor Daniel, Inc., Nuclear Material Storage Facility renovation design (initial 30% of Title 1). Thirteen facility cases were evaluated with varying temperature dependence, drywell-array heat-source magnitude and distribution, location of the inlet tower, and no-flow curtains in the drywell-array vault. Four cases of a detailed model of the inlet-tower top fixture were evaluated to show the effect of the canopy-cruciform fixture design on the air pressure and flow distributions

Use of the water-soluble fluor sodium salicylate for fluorographic detection of tritium in thin-layer chromatograms and nitrocellulose blots

International Nuclear Information System (INIS)

Lucher, L.A.; Lego, T.

1989-01-01

We have determined that sodium salicylate, a water-soluble fluor which we use routinely for fluorography with polyacrylamide gels, is also useful for fluorography with thin-layer media. Detection of 3 H-labeled material applied to thin-layer chromatography plates, or nitrocellulose membranes, can be enhanced up to 150-fold after treatment with an aqueous solution of 2 M sodium salicylate, while detection of 35 S-labeled material is enhanced only about 2-fold. We demonstrate the utility of sodium salicylate fluorography in detecting 3H-labeled palmitic acid following thin-layer chromatography and 3 H-labeled proteins following blotting to nitrocellulose

In Vitro and In Vivo Evaluation of Alexa Fluor 680-Bombesin[7–14]NH2 Peptide Conjugate, a High-Affinity Fluorescent Probe with High Selectivity for the Gastrin-Releasing Peptide Receptor

Directory of Open Access Journals (Sweden)

Lixin Ma

2007-05-01

Full Text Available Gastrin-releasing peptide (GRP receptors are overexpressed on several types of human cancer cells, including breast, prostate, small cell lung, and pancreatic cancers. Bombesin (BBN, a 14–amino acid peptide that is an analogue of human GRP, binds to GRP receptors with very high affinity and specificity. The aim of this study was to develop a new fluorescent probe based on BBN having high tumor uptake and optimal pharmacokinetics for specific targeting and optical imaging of human breast cancer tissue. In this study, solid-phase peptide synthesis was used to produce H2N-glycylglycylglycine-BBN[7–14]NH2 peptide with the following general sequence: H2N-G-G-G-Q-W-A-V-G-H-L-M-(NH2. This conjugate was purified by reversed-phase high-performance liquid chromatography and characterized by electrospray-ionization mass spectra. The fluorescent probe Alexa Fluor 680-G-G-G-BBN[7–14]NH2 conjugate was prepared by reaction of Alexa Fluor 680 succinimidyl ester to H2N-G-G-G-BBN[7–14]NH2 in dimethylformamide (DMF. In vitro competitive binding assays, using 125I-Tyr4-BBN as the radiolabeling gold standard, demonstrated an inhibitory concentration 50% value of 7.7 ± 1.4 nM in human T-47D breast cancer cells. Confocal fluorescence microscopy images of Alexa Fluor 680-G-G-G-BBN[7–14]NH2 in human T-47D breast cancer cells indicated specific uptake, internalization, and receptor blocking of the fluorescent bioprobe in vitro. In vivo investigations in SCID mice bearing xenografted T-47D breast cancer lesions demonstrated the ability of this new conjugate to specifically target tumor tissue with high selectivity and affinity.

Bi-layered zirconia/fluor-apatite bridges supported by ceramic dental implants: a prospective case series after thirty months of observation.

Science.gov (United States)

Spies, Benedikt Christopher; Witkowski, Siegbert; Butz, Frank; Vach, Kirstin; Kohal, Ralf-Joachim

2016-10-01

The aim of this study was to determine the success and survival rate of all-ceramic bi-layered implant-supported three-unit fixed dental prostheses (IS-FDPs) 3 years after implant placement. Thirteen patients (seven males, six females; age: 41-78 years) received two one-piece ceramic implants (alumina-toughened zirconia) each in the region of the premolars or the first molar and were finally restored with adhesively cemented bi-layered zirconia-based IS-FDPs (3 in the maxilla, 10 in the mandible) composed of CAD/CAM-fabricated zirconia frameworks pressed-over with fluor-apatite glass-ceramic ingots. At prosthetic delivery and the follow-ups after 1, 2 and 3 years, the restorations were evaluated using modified United States Public Health Service (USPHS) criteria. Restorations with minor veneer chippings, a small-area occlusal roughness, slightly soundable restoration margins, minimal contour deficiencies and tolerable color deviations were regarded as success. In case of more distinct defects that could, however, be repaired to a clinically acceptable level, IS-FDPs were regarded as surviving. Kaplan-Meier plots were used for the success/survival analyses. To verify an impact on subjective patients' perceptions, satisfaction was evaluated by visual analog scales (VAS). All patients were seen 3 years after implant installation. No IS-FDP had to be replaced, resulting in 100% survival after a mean observation period of 29.5 months (median: 30.7). At the 3-year follow-up, 7/13 IS-FDPs showed a veneer chipping, 13/13 an occlusal roughness and 12/13 minimal deficiencies of contour/color. Since six restorations showed a major chipping and/or a major occlusal roughness, the Kaplan-Meier success rate was 53.8%. However, patients' significantly improved perceptions of function, esthetics, sense, and speech at prosthetic delivery remained stable over time. Bi-layered zirconia/fluor-apatite IS-FDPs entirely survived the observation period but showed a high frequency of

Facile green synthesis of silver doped fluor-hydroxyapatite/β-cyclodextrin nanocomposite in the dual acting fluorine-containing ionic liquid medium for bone substitute applications

Energy Technology Data Exchange (ETDEWEB)

Jegatheeswaran, S. [Advanced Green Chemistry Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi-3, Tamil Nadu (India); Selvam, S. [Laser and Sensor Application Laboratory, Pusan National University, Busan 609735 (Korea, Republic of); Sri Ramkumar, V. [Deptartment of Environmental Biotechnology, School of Environmental, Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu (India); Sundrarajan, M., E-mail: sundrarajan@yahoo.com [Advanced Green Chemistry Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi-3, Tamil Nadu (India)

2016-05-15

Highlights: • Fluorine based ionic liquid was highly influenced the morphological structure of nanocomposites. • These composites has been motivated controlled release of silver nanoparticles for uniform antibacterial activity. • These material has given excellent antibacterial biofilm activity and favourable cytotoxical behavior on the human osteosarcoma (MG-63) cells. • These material has been highly suitable for bone substitute appliactions. - Abstract: A novel green route has approached for the synthesis of silver doped fluor-hydroxyapatite/β-cyclodextrin composite by the assistance of fluorine-based ionic liquid. The selected [BMIM]BF{sub 4} ionic liquid for this work plays a dual role as fluoride source and templating agent. It helps to improve the crystalline structures and the shape of the composites. The crystallinity, surface morphology, topographical studies of the synthesized composite were validated. The XRD results of the composite show typical Ag reflection peaks at 38.1°, 44.2° and 63.4°. The ionic liquid assisted composite displayed the hexagonal shaped HA particles, which are surrounded by spherical nano-Ag particles and these particles are uniformly dispersed in the β-cyclodextrin matrix in both horizontal and cross sections from surface morphology observations. The Ionic liquid assisted silver doped fluor-hydroxyapatite/β-cyclodextrin composite exhibited very good antibacterial activities against Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Serratia liquefaciens pathogens. The antibacterial proficiencies were established using Confocal Laser Scanning Microscopic developed biofilms images and bacterial growth curve analysis. The cytotoxicity results of the ionic liquid assisted composite analyzed by cell proliferation in vitro studies using human osteosarcoma cell line (MG-63) and this study has shown excellent biocompatibility.

Facile green synthesis of silver doped fluor-hydroxyapatite/β-cyclodextrin nanocomposite in the dual acting fluorine-containing ionic liquid medium for bone substitute applications

International Nuclear Information System (INIS)

Jegatheeswaran, S.; Selvam, S.; Sri Ramkumar, V.; Sundrarajan, M.

2016-01-01

Highlights: • Fluorine based ionic liquid was highly influenced the morphological structure of nanocomposites. • These composites has been motivated controlled release of silver nanoparticles for uniform antibacterial activity. • These material has given excellent antibacterial biofilm activity and favourable cytotoxical behavior on the human osteosarcoma (MG-63) cells. • These material has been highly suitable for bone substitute appliactions. - Abstract: A novel green route has approached for the synthesis of silver doped fluor-hydroxyapatite/β-cyclodextrin composite by the assistance of fluorine-based ionic liquid. The selected [BMIM]BF_4 ionic liquid for this work plays a dual role as fluoride source and templating agent. It helps to improve the crystalline structures and the shape of the composites. The crystallinity, surface morphology, topographical studies of the synthesized composite were validated. The XRD results of the composite show typical Ag reflection peaks at 38.1°, 44.2° and 63.4°. The ionic liquid assisted composite displayed the hexagonal shaped HA particles, which are surrounded by spherical nano-Ag particles and these particles are uniformly dispersed in the β-cyclodextrin matrix in both horizontal and cross sections from surface morphology observations. The Ionic liquid assisted silver doped fluor-hydroxyapatite/β-cyclodextrin composite exhibited very good antibacterial activities against Escherichia coli, Salmonella typhi, Klebsiella pneumonia and Serratia liquefaciens pathogens. The antibacterial proficiencies were established using Confocal Laser Scanning Microscopic developed biofilms images and bacterial growth curve analysis. The cytotoxicity results of the ionic liquid assisted composite analyzed by cell proliferation in vitro studies using human osteosarcoma cell line (MG-63) and this study has shown excellent biocompatibility.

Study of the use of an electric discharge for hollow cathodes used as optical excitation sources in the spectrographic measurement of fluorine in thorium, uranium and plutonium; Etude de l'utilisation de la decharge electrique en cathode creuse comme source d'excitation optique pour le dosage spectrographique du fluor dans le thorium, l'uranium et le plutonium

Energy Technology Data Exchange (ETDEWEB)

Bufpereau, M; Crehange, G; Poublan, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1964-07-01

Previous works and phenomena concerned with a hollow cathode excitation are reviewed. Experiments aimed specially on the determination of the best conditions for an analysis of fluorine in oxides-metals and solutions. In that purpose, several factors have been pointed out. One started some researches about others elements that fluorine. Carrying fluorine into discharge and excitation have been more specially studied. A quantitative analysis method is given. The analysis limit is 45 ppm about but the detection limit is 5 ppm about. As a conclusion, various ways for optical excitation of fluorine are reviewed as other analytical possibilities a hollow cathode discharge offers. (authors) [French] On rappelle les travaux effectues jusqu'alors ainsi que les phenomenes mis en jeu dans l'excitation cathode creuse. Les experiences effectuees ont eu pour but essentiel la determination des conditions optima du dosage du fluor dans les oxydes, metaux et solutions. Pour cela de nombreux facteurs ont ete mis en evidence. Certaines etudes concernant d'autres elements que le fluor ont ete amorcees. Le passage du fluor dans la decharge et son excitation ont ete plus particulierement etudies. Une methode d'analyse quantitative est degagee, la limite de dosage est de l'ordre de 45 ppm, la limite de detection de 5 ppm. En conclusion, on passe en revue les differentes methodes d'excitation optique du fluor ainsi que les autres possibilites analytiques que peut offrir la cathode creuse. (auteurs)

Amine Analysis Using AlexaFluor 488 Succinimidyl Ester and Capillary Electrophoresis with Laser-Induced Fluorescence

Directory of Open Access Journals (Sweden)

Christian G. Kendall

2015-01-01

Full Text Available Fluorescent probes enable detection of otherwise nonfluorescent species via highly sensitive laser-induced fluorescence. Organic amines are predominantly nonfluorescent and are of analytical interest in agricultural and food science, biomedical applications, and biowarfare detection. Alexa Fluor 488 N-hydroxysuccinimidyl ester (AF488 NHS-ester is an amine-specific fluorescent probe. Here, we demonstrate low limit of detection of long-chain (C9 to C18 primary amines and optimize AF488 derivatization of long-chain primary amines. The reaction was found to be equally efficient in all solvents studied (dimethylsulfoxide, ethanol, and N,N-dimethylformamide. While an organic base (N,N-diisopropylethylamine is required to achieve efficient reaction between AF488 NHS-ester and organic amines with longer hydrophobic chains, high concentrations (>5 mM result in increased levels of ethylamine and propylamine in the blank. Optimal incubation times were found to be >12 hrs at room temperature. We present an initial capillary electrophoresis separation for analysis using a simple micellar electrokinetic chromatography (MEKC buffer consisting of 12 mM sodium dodecylsulfate (SDS and 5 mM carbonate, pH 10. Limits of detection using the optimized labeling conditions and these separation conditions were 5–17 nM. The method presented here represents a novel addition to the arsenal of fluorescent probes available for highly sensitive analysis of small organic molecules.

Fluor Daniel Hanford implementation plan for DOE Order 5480.28. Natural phenomena hazards mitigation

International Nuclear Information System (INIS)

Conrads, T.J.

1997-01-01

Natural phenomena hazards (NPH) are unexpected acts of nature that pose a threat or danger to workers, the public, or the environment. Earthquakes, extreme winds (hurricane and tornado), snow, flooding, volcanic ashfall, and lightning strikes are examples of NPH that could occur at the Hanford Site. U.S. Department of Energy (DOE) policy requires facilities to be designed, constructed, and operated in a manner that protects workers, the public, and the environment from hazards caused by natural phenomena. DOE Order 5480.28, Natural Phenomena Hazards Mitigation, includes rigorous new natural phenomena criteria for the design of new DOE facilities, as well as for the evaluation and, if necessary, upgrade of existing DOE facilities. The Order was transmitted to Westinghouse Hanford Company in 1993 for compliance and is also identified in the Project Hanford Management Contract, Section J, Appendix C. Criteria and requirements of DOE Order 5480.28 are included in five standards, the last of which, DOE-STD-1023, was released in fiscal year 1996. Because the Order was released before all of its required standards were released, enforcement of the Order was waived pending release of the last standard and determination of an in-force date by DOE Richland Operations Office (DOE-RL). Agreement also was reached between the Management and Operations Contractor and DOE-RL that the Order would become enforceable for new structures, systems, and components (SSCS) 60 days following issue of a new order-based design criteria in HNF-PRO-97, Engineering Design and Evaluation. The order also requires that commitments addressing existing SSCs be included in an implementation plan that is to be issued 1 year following the release of the last standard. Subsequently, WHC-SP-1175, Westinghouse Hanford Company Implementation Plan for DOE Order 5480.28, Natural Phenomena Hazards Mitigation, Rev. 0, was issued in November 1996, and this document, HNF-SP-1175, Fluor Daniel Hanford

Hydroxyapatite, fluor-hydroxyapatite and fluorapatite produced via the sol-gel method: bonding to titanium and scanning electron microscopy.

Science.gov (United States)

Tredwin, Christopher J; Georgiou, George; Kim, Hae-Won; Knowles, Jonathan C

2013-05-01

Hydroxyapatite (HA), fluor-hydroxyapatite (FHA) with varying levels of fluoride ion substitution and fluorapatite (FA) production has been characterised and optimised by the sol-gel method and the dissolution and biological properties of these materials were investigated. It was the objective of this study to investigate the potential bond strength and interaction of these materials with titanium. HA, FHA and FA were synthesised by a sol-gel method. Calcium nitrate and triethyl phosphite were used as precursors under an ethanol-water based solution. Different amounts of ammonium fluoride (NH4F) were incorporated for the preparation of the FHA and FA sol-gels. Using a spin coating technique the sol-gels were coated onto commercially pure titanium disks and crystallised at various temperatures. Using scanning electron microscopy (SEM) and elemental analysis, the surface characteristics, coating thickness and interaction of the Ti substrate and coating were investigated. The bond strengths of the coating to the Ti were investigated using an Instron Universal Load Testing Machine. Statistical analysis was performed with a two-way analysis of variance and post hoc testing with a Bonferroni correction. (1) Coating speed inversely influenced the coating thickness. (2) Increasing fluoride ion substitution and heating temperature significantly increased bond strength and (3) increasing fluoride ion substitution increased the coating thickness. FHA and FA synthesised using the sol-gel technique may offer a superior alternative to coating titanium implants with HA and plasma spraying. HA, FHA and FA materials synthesised by the sol-gel method may also have a use as bone grafting materials. Copyright © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Applications of Fluorine-18 in Biological Studies with Special Reference to Bone and Thyroid Physiology; Emploi du Fluor-18 dans des Études Biologiques, Notamment sur la Physiologie des Os et de la Thyroïde; ИСПОЛЬЗОВАНИЕ ФТОРА-18 В БИОЛОГИЧЕСКИХ ИССЛЕДОВАНИЯХ С УДЕЛЕНИЕМ ОСОБОГО ВНИМАНИЯ ВОПРОСАМ ФИЗИОЛОГИИ КОСТЕЙ И ФИЗИОЛОГИИ ПИТОВИДНОЙ ЖЕЛЕЗЫ; Aplicaciones del Fluor-18 en Estudios Biologicos, con Especial Referencia a la Fisiologia del Esqueleto y de la Tiroides

Energy Technology Data Exchange (ETDEWEB)

Anbar, M. [Weizmann Institute of Science (Israel); IAEC Soreq Research Establishment, Rehovot (Israel)

1963-03-15

the trapping of fluoroborate, although they diminish the overall iodine uptake. Next, it was demonstrated that the trapping stage is much less radiosensitive than the stages of thyroxine formation and release. A quite different application of F{sup 16}-labelled fluoroborate ions was in the localization of brain tumours, by directional coincidence scanning owing to the limited permeability of these ions via the intact blood- brain barrier. Fluorine-18 labelled fluoraromatic chelating agents were prepared and applied to problems in bone physiology. Fluorine-18 labelled aromatic vital dyes were used in investigating problems of permeability through biological membranes. Fluorine-18 labelled fluorine containing antimetabolites including 5-fluorouracyl and fluoro-orotic acid, have been prepared and applied to physiological problems, including cancer research. (author) [French] Dans les laboratoires de la CEAI, le fluor-18 a ete utilise au cours des trois dernieres annees pour resoudre de nombreux problemes de biologie et de medecine. Des methodes ont ete mises au point pour preparer le fluor par chacune des reactions {sup 18}O (p, n), {sup 16}O(H{sup 3}, n) et {sup 18}F(n, 2n). Des composes marques au radiofluor ont ete prepares par echange isotopique, synthese, marquage par recul et retention du fluor dans des composes fluores organiques soumis a la reaction (n, 2n). Des techniques speciales de comptage ont ete mises au point pour mesurer les faibles activites de doses traceuses de composes fluores organiques. Les ions {sup 18}F{sup -} ont ete utilises pour des etudes sur la physiologie des os. On a constate que l'ion F' est semblable au calcium en de nombreux aspects de son comportement physiologique; on a observe que la fixation F par l'os augmente sous l'influence de la vitamine D et de la testosterone tandis que la cortisone et les oestrogenes reduisent le taux de fixation. Le mode de distribution du fluor dans l'organisme se modifie lorsqu'il est administre sous la

Autofluorogram and composition and method for making the same

International Nuclear Information System (INIS)

Fost, D.L.

1980-01-01

A highly sensitive and efficient autofluorographic technique for weak radioactive emitters such as tritium or 14 C is described which involves impregnating an autofluorographic medium with a fluor in a carboxylic acid carrier. The autofluorographic effect on film of radioactive emitters contained within a medium is enhanced by contacting the medium with a fluor dissolved or dispersed in a water-soluble or water-miscible carboxylic acid to disperse the fluor throughout the medium, and precipitating the fluor. (U.K.)

Adsorption of volatile organic compounds by polytetra-fluor ethylene; Adsorption de composes organiques volatils par le polytetrafluor ethylene

Energy Technology Data Exchange (ETDEWEB)

Martinet, J.M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1958-07-01

The sorption of organic vapours by microporous polytetra-fluor ethylene has been studied gravimetrically using a Mc Bain-Baker type sorption balance. The amount of sorption, the peculiarities observed on the isotherm curves, the small influence of temperature, and smallness of hysteresis suggests that mainly physical adsorption occurs when the temperature is around 25 deg. C. The values of the surface areas obtained from the adsorption isotherms using organic vapours differ greatly from those derived from N{sub 2} adsorption measurements. This discrepancy cannot be completely attributed to differences in the structure and chemical function of the adsorbate molecules, or to the porous structure of the adsorbent. On the contrary, the surface area values obtained by sorbing high volatile freons conform with those measured by nitrogen adsorption, which seems to imply a connection between the area of sorbed monolayers and volatility of the adsorbate. (author) [French] La sorption de vapeurs organiques par du polytetrafluor ethylene microporeux a ete etudiee gravimetriquement a l'aide d'un appareillage du type balance de Mac Bain. La valeur de la masse adsorbee, les particularites observees dans la forme des isothermes, le peu d'influence de la temperature, la faiblesse de l'hysteresis suggerent l'intervention d'une adsorption physique, du moins au voisinage de 25 deg. C. Les isothermes relatives a l'absorption de vapeurs organiques conduisent a des valeurs de la surface specifique tres differentes de celles obtenues par adsorption d'azote. Ces divergences ne peuvent s'expliquer par la seule intervention de la structure moleculaire et de la fonction chimique de l'adsorbat, ni par la structure poreuse de l'adsorbant. Par contre, l'adsorption de freons tres volatils conduit a des valeurs de la surface specifique analogues a celles obtenues par adsorption d'azote ce qui semble etablir un lien entre la

Adsorption of volatile organic compounds by polytetra-fluor ethylene; Adsorption de composes organiques volatils par le polytetrafluor ethylene

Energy Technology Data Exchange (ETDEWEB)

Martinet, J M [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1958-07-01

The sorption of organic vapours by microporous polytetra-fluor ethylene has been studied gravimetrically using a Mc Bain-Baker type sorption balance. The amount of sorption, the peculiarities observed on the isotherm curves, the small influence of temperature, and smallness of hysteresis suggests that mainly physical adsorption occurs when the temperature is around 25 deg. C. The values of the surface areas obtained from the adsorption isotherms using organic vapours differ greatly from those derived from N{sub 2} adsorption measurements. This discrepancy cannot be completely attributed to differences in the structure and chemical function of the adsorbate molecules, or to the porous structure of the adsorbent. On the contrary, the surface area values obtained by sorbing high volatile freons conform with those measured by nitrogen adsorption, which seems to imply a connection between the area of sorbed monolayers and volatility of the adsorbate. (author) [French] La sorption de vapeurs organiques par du polytetrafluor ethylene microporeux a ete etudiee gravimetriquement a l'aide d'un appareillage du type balance de Mac Bain. La valeur de la masse adsorbee, les particularites observees dans la forme des isothermes, le peu d'influence de la temperature, la faiblesse de l'hysteresis suggerent l'intervention d'une adsorption physique, du moins au voisinage de 25 deg. C. Les isothermes relatives a l'absorption de vapeurs organiques conduisent a des valeurs de la surface specifique tres differentes de celles obtenues par adsorption d'azote. Ces divergences ne peuvent s'expliquer par la seule intervention de la structure moleculaire et de la fonction chimique de l'adsorbat, ni par la structure poreuse de l'adsorbant. Par contre, l'adsorption de freons tres volatils conduit a des valeurs de la surface specifique analogues a celles obtenues par adsorption d'azote ce qui semble etablir un lien entre la volatilite de l'adsorbat et l'etendue des couches monomoleculaires

Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores.

Science.gov (United States)

Liao-Chan, Sindy; Daine-Matsuoka, Barbara; Heald, Nathan; Wong, Tiffany; Lin, Tracey; Cai, Allen G; Lai, Michelle; D'Alessio, Joseph A; Theunissen, Jan-Willem

2015-01-01

Antibodies against cell surface antigens may be internalized through their specific interactions with these proteins and in some cases may induce or perturb antigen internalization. The anti-cancer efficacy of antibody-drug conjugates is thought to rely on their uptake by cancer cells expressing the surface antigen. Numerous techniques, including microscopy and flow cytometry, have been used to identify antibodies with desired cellular uptake rates. To enable quantitative measurements of internalization of labeled antibodies, an assay based on internalized and quenched fluorescence was developed. For this approach, we generated novel anti-Alexa Fluor monoclonal antibodies (mAbs) that effectively and specifically quench cell surface-bound Alexa Fluor 488 or Alexa Fluor 594 fluorescence. Utilizing Alexa Fluor-labeled mAbs against the EphA2 receptor tyrosine kinase, we showed that the anti-Alexa Fluor reagents could be used to monitor internalization quantitatively over time. The anti-Alexa Fluor mAbs were also validated in a proof of concept dual-label internalization assay with simultaneous exposure of cells to two different mAbs. Importantly, the unique anti-Alexa Fluor mAbs described here may also enable other single- and dual-label experiments, including label detection and signal enhancement in macromolecules, trafficking of proteins and microorganisms, and cell migration and morphology.

Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores.

Directory of Open Access Journals (Sweden)

Sindy Liao-Chan

Full Text Available Antibodies against cell surface antigens may be internalized through their specific interactions with these proteins and in some cases may induce or perturb antigen internalization. The anti-cancer efficacy of antibody-drug conjugates is thought to rely on their uptake by cancer cells expressing the surface antigen. Numerous techniques, including microscopy and flow cytometry, have been used to identify antibodies with desired cellular uptake rates. To enable quantitative measurements of internalization of labeled antibodies, an assay based on internalized and quenched fluorescence was developed. For this approach, we generated novel anti-Alexa Fluor monoclonal antibodies (mAbs that effectively and specifically quench cell surface-bound Alexa Fluor 488 or Alexa Fluor 594 fluorescence. Utilizing Alexa Fluor-labeled mAbs against the EphA2 receptor tyrosine kinase, we showed that the anti-Alexa Fluor reagents could be used to monitor internalization quantitatively over time. The anti-Alexa Fluor mAbs were also validated in a proof of concept dual-label internalization assay with simultaneous exposure of cells to two different mAbs. Importantly, the unique anti-Alexa Fluor mAbs described here may also enable other single- and dual-label experiments, including label detection and signal enhancement in macromolecules, trafficking of proteins and microorganisms, and cell migration and morphology.

Fernald - Developing and Executing an Accelerated Closure Plan

International Nuclear Information System (INIS)

Nixon, D.A.

2006-01-01

In November 2000 the Department of Energy (DOE) and Fluor Fernald entered into a closure contract that incited Fluor Fernald to reduce the cost and schedule of the Fernald site cleanup. The contract established a target schedule and target cost and how Fluor Fernald performs against these targets determines the amount of fee the company earns. In response to these new challenges, Fluor Fernald developed a 13-part strategy to safely accelerate work and more efficiently utilize the available funding. Implementation of this strategy required a dramatic culture change at Fernald - from a 'government job mind set' to an entrepreneurial/commercial model. Fluor Fernald's strategy and culture change has proved to be successful as the company is on track to close the site ahead of the target schedule at a total project cost less than the target cost. The elements of Fluor Fernald's strategy and the lessons learned during implementation provide valuable information that could be utilized by other DOE sites that will be undergoing closure over the next decade. (authors)

THE FLUORBOARD A STATISTICALLY BASED DASHBOARD METHOD FOR IMPROVING SAFETY

International Nuclear Information System (INIS)

PREVETTE, S.S.

2005-01-01

The FluorBoard is a statistically based dashboard method for improving safety. Fluor Hanford has achieved significant safety improvements--including more than a 80% reduction in OSHA cases per 200,000 hours, during its work at the US Department of Energy's Hanford Site in Washington state. The massive project on the former nuclear materials production site is considered one of the largest environmental cleanup projects in the world. Fluor Hanford's safety improvements were achieved by a committed partnering of workers, managers, and statistical methodology. Safety achievements at the site have been due to a systematic approach to safety. This includes excellent cooperation between the field workers, the safety professionals, and management through OSHA Voluntary Protection Program principles. Fluor corporate values are centered around safety, and safety excellence is important for every manager in every project. In addition, Fluor Hanford has utilized a rigorous approach to using its safety statistics, based upon Dr. Shewhart's control charts, and Dr. Deming's management and quality methods

Development of long-wavelength-emitting scintillators with improved decay time characteristics

International Nuclear Information System (INIS)

Franks, L.A.; Lutz, S.; Lyons, P.B.

1978-01-01

Progress is reported from efforts to develop radiation-to-light converters suitable for use with optical fibers as they are applied to the diagnostics of transient nuclear phenomena. Liquid and plastic fluors have been prepared which emit in the 550- to 600-nm region. Ternary liquid systems with decay times as short as 1.3 ns at 560 nm and plastic fluors with decay times less than 3 ns at 560 nm are reported. Other liquid and plastic fluors are reported with improved emission characteristics in the region of 600 nm. Conversion efficiences, on a pulse amplitude basis, are generally lower than that of a commercially available 570 nm-16 ns plastic fluor

Design Compliance Matrices to ANSI and OSHA

International Nuclear Information System (INIS)

BENDIXSEN, R.B.

2000-01-01

U.S. Department of Energy Letter 98-SFD-028 requested Fluor Daniel Hanford, Inc. to provide clarifications as to compliance with ANSI 57.1, 57.2, 57.9, and 29 CFR 1910.179 (OSHA), in the form of an item-by-item compliance matrix, for the CSB. This Supporting Document contains Fluor Daniel, Inc.'s response for use by Fluor Daniel Hanford, Inc. regarding the clarifications requested by the U.S. Department of Energy

ELECTROD FLUOR-SELECTIV

Directory of Open Access Journals (Sweden)

Mariana DÎRU

2018-03-01

Full Text Available A fost preparat un senzor anionic specific, bazat pe pivalatul trinuclear al cromului(III ca material electro­activ încorporat în membrana PVC plastifiată. Senzorul prezintă răspuns Nernstian (55,78 mV/decadă în intervalul de concentrație 10-1-10-4 mol/L cu limita de detecție 2,0∙10-5 mol/L pentru anionul fluorură. Domeniul optim de pH de funcţionare a electrodului asamblat este ˃5. Senzorul dat are un timp de răspuns de 30-60 s și reproductibilitatea rezultatelor se menține timp de 3 luni. Coeficienții potențiometrici ai selectivității au fost determinați prin metoda soluțiilor separate. A fost realizată aplicarea acestor electrozi la analiza pastei de dinți ce conține fluorură și rezultatele experimentale au fost comparate cu datele de pe prospect.FLUORIDE-SELECTIVE ELECTRODEA specific anionic sensor has been prepared, based on trinuclearchromium(III pivalate as sensing material incorpo­rated into the plasticized PVC-membrane. The sensor exhibited Nernstian response (55,78 mV/decade in the region between 10-1-10-4 mol/L with a detection limit of 2,0∙10-5 mol/L for fluoride. The working pH of the electrode was in the 5-6 range. The sensor has a response time 30-60 s and can be used for least 3 month. The potentiometric selectivity coefficients were determined by separate solution method. Application of these electrodes to the analysis of toothpaste containing fluoride has been realized and experimental results have been compared with the data on the prospectus.

Study of a micro-sublimation apparatus with removal of the vapours by pumping; application to the analysis of fluorinated products (1963); Etude d'un appareillage de microsublimation avec entrainement des vapeurs par pompage et application a l'analyse des produits fluores (1963)

Energy Technology Data Exchange (ETDEWEB)

Delvalle, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1963-07-01

Micro-sublimation analysis presents definite advantages both from the qualitative and quantitative points of view. An automatic micro-sublimation analysis apparatus has been developed for the analysis of fluorinated products (ClF{sub 3}, HF, UF{sub 6}, etc.) but this is only one particular application of a method which has a far wider field of possible applications. We give first the most favorable conditions for the operation of such an apparatus. These conditions are the use of a detector which is linear and independent of the nature of the gas, the flow of the sublimed vapours in the conditions of molecular flow, and finally a reproducible and linear re-heating of the separating trap. The apparatus thus built has the advantage of yielding any analysis without prior calibration. It also makes possible the easy identification of an unknown product by the determination of its vapour pressure curve and its molecular weight. The analysis of fluorinated products with this apparatus has shown that the experimental results agree well with what is expected. (author) [French] L'analyse par microsublimation presente un interet certain tant au point de vue qualitatif que quantitatif. Nous avons mis au point un appareillage automatique d'analyse par microsublimation pour l'analyse des produits fluores (CIF{sub 3}, HF, UF{sub 6}, etc.) mais il ne s'agit que de l'application a un cas particulier d'une methode ayant un champ d'applications bien plus vaste. Nous exposons tout d'abord les conditions les plus favorables au bon fonctionnement d'un tel appareil. Ces conditions sont l'emploi d'un detecteur lineaire et independant de la nature du gaz, l'ecoulement des vapeurs sublimees en regime moleculaire et enfin un rechauffage reproductible et lineaire du piege separateur. L'appareil ainsi realise presente l'avantage d'effectuer une analyse quelconque sans etalonnage prealable. Il permet en outre d'identifier aisement un corps inconnu par la determination de sa courbe de tension

NAVIGATING A QUALITY ROUTE TO A NATIONAL SAFETY AWARD

Energy Technology Data Exchange (ETDEWEB)

PREVETTE SS

2009-05-26

Deming quality methodologies applied to safety are recognized with the National Safety Council's annual Robert W. Campbell Award. Over the last ten years, the implementation of Statistical Process Control and quality methodologies at the U.S. Department of Energy's Hanford Site have contributed to improved safety. Improvements attributed to Statistical Process Control are evidenced in Occupational Safety and Health records and documented through several articles in Quality Progress and the American Society of Safety Engineers publication, Professional Safety. Statistical trending of safety, quality, and occurrence data continues to playa key role in improving safety and quality at what has been called the world's largest environmental cleanup project. DOE's Hanford Site played a pivotal role in the nation's defense beginning in the 1940s, when it was established as part of the Manhattan Project. After more than 50 years of producing material for nuclear weapons, Hanford, which covers 586 square miles in southeastern Washington state, is now focused on three outcomes: (1) Restoring the Columbia River corridor for multiple uses; (2) Transitioning the central plateau to support long-term waste management; and (3) Putting DOE assets to work for the future. The current environmental cleanup mission faces challenges of overlapping technical, political, regulatory, environmental, and cultural interests. From Oct. 1, 1996 through Sept. 30, 2008, Fluor Hanford was a prime contractor to the Department of Energy's Richland Operations Office. In this role, Fluor Hanford managed several major cleanup activities that included dismantling former nuclear-processing facilities, cleaning up the Site's contaminated groundwater, retrieving and processing transuranic waste for shipment and disposal off-site, maintaining the Site's infrastructure, providing security and fire protection, and operating the Volpentest HAMMER Training and Education

Correlative FRET: new method improves rigor and reproducibility in determining distances within synaptic nanoscale architecture

Science.gov (United States)

Shinogle-Decker, Heather; Martinez-Rivera, Noraida; O'Brien, John; Powell, Richard D.; Joshi, Vishwas N.; Connell, Samuel; Rosa-Molinar, Eduardo

2018-02-01

A new correlative Förster Resonance Energy Transfer (FRET) microscopy method using FluoroNanogold™, a fluorescent immunoprobe with a covalently attached Nanogold® particle (1.4nm Au), overcomes resolution limitations in determining distances within synaptic nanoscale architecture. FRET by acceptor photobleaching has long been used as a method to increase fluorescence resolution. The transfer of energy from a donor to an acceptor generally occurs between 10-100Å, which is the relative distance between the donor molecule and the acceptor molecule. For the correlative FRET microscopy method using FluoroNanogold™, we immuno-labeled GFP-tagged-HeLa-expressing Connexin 35 (Cx35) with anti-GFP and with anti-Cx35/36 antibodies, and then photo-bleached the Cx before processing the sample for electron microscopic imaging. Preliminary studies reveal the use of Alexa Fluor® 594 FluoroNanogold™ slightly increases FRET distance to 70Å, in contrast to the 62.5Å using AlexaFluor 594®. Preliminary studies also show that using a FluoroNanogold™ probe inhibits photobleaching. After one photobleaching session, Alexa Fluor 594® fluorescence dropped to 19% of its original fluorescence; in contrast, after one photobleaching session, Alexa Fluor 594® FluoroNanogold™ fluorescence dropped to 53% of its original intensity. This result confirms that Alexa Fluor 594® FluoroNanogold™ is a much better donor probe than is Alexa Fluor 594®. The new method (a) creates a double confirmation method in determining structure and orientation of synaptic architecture, (b) allows development of a two-dimensional in vitro model to be used for precise testing of multiple parameters, and (c) increases throughput. Future work will include development of FluoroNanogold™ probes with different sizes of gold for additional correlative microscopy studies.

PHYSICO-CHEMICAL CHARACTERISTICS AND QUALITY OF FEED WATERS OF THE EL-OUED CITY STUDY OF CASE (fluorine and Arsenic

Directory of Open Access Journals (Sweden)

S. E. Laouini

2015-08-01

Full Text Available In these days, of numerous country through worlds are confronted to an excess of fluor and arsenic in the wake of drink and preoccupy by in to minimize consequences on the consumers health. The arsenic and the fluor are to scale planetary polluted them of geological origin more frequently met in underground waters. In of numerous countries in expansion, waters so contaminated are often consummated without any previous treatment. Effects inorganic reserved salts in the wake of drink, benefic or luckless acting of their concentration, are known since of numerous years today already more 1,5 billion of persons have not attack to a healthy drinking-water. According to the OMS, these are 15 million to be human who die each year after to drink of the water no potable, or for want of not to have not attack to the drinking-water . Of numerous countries being in possession regions where some waters present amounts in fluor and arsenic superior to recommendations of the OMS (0,7-1,5 mg/L for fluor and 10 L/g for the arsenic. The fluor behoves against blights and hardens the teeth enamel ;to better concentration it fragilise the bone or induces points of crystallization favoring the appearance of articular distorting arthrosis : this is her fluorose. Effects ought to the arsenic are numerous: lésions of the peel, canker, illnesses cardiovasculair or pulmonarias, hypertension and cancers.This work has been realized on the water of five boreholes of the town of El-Oued in collaboration with the laboratory of the waters Algerian (ADE.The experimentation was aiming to define them plenty of fluor and arsenic exists in five boreholes to study and in fine to estimate the quality consumptive waters.

Early Life Stress and Sleep Restriction as Risk Factors in PTSD: An Integrative Pre-Clinical Approach

Science.gov (United States)

2014-04-01

month later rats were re-exposed to the context of the WAZM while their behavior was video recorded. Furthermore, c-Fos expression in the amygdala...Alexa Fluor 555 donkey α rabbit 1:1000 + Alexa Fluor 488 donkey α mouse 1:1000; both Invitrogen, Carlsbad, CA, USA) in 3% BSA + 0.3% TritonX + PBS for

Radioactive waste isolation in salt: Peer review of the Fluor Technology, Inc., report and position paper concerning waste emplacement mode and its effect on repository conceptual design

International Nuclear Information System (INIS)

Hambley, D.F.; Russell, J.E.; Whitfield, R.G.

1987-02-01

Recommendations for revising the Fluor Technology, Inc., draft position paper entitled Evaluation of Waste Emplacement Mode and the final report entitled Waste Package/Repository Impact Study include: reevaluate the relative rankings for the various emplacement modes; delete the following want objectives: maximize ability to locate the package horizon because sufficient flexibility exists to locate rooms in the relatively clean San Andres Unit 4 Salt and maximize far-field geologic integrity during retrieval because by definition the far field will be unaffected by thermal and stress perturbations caused by remining; give greater emphasis to want objectives regarding cost and use of present technology; delete the following statements from pages 1-1 and 1-2 of the draft position paper: ''No thought or study was given to the impacts of this configuration [vertical emplacement] on repository construction or short and long-term performance of the site'' and ''Subsequent salt repository designs adopted the vertical emplacement configuration as the accepted method without further evaluation.''; delete App. E and lines 8-17 of page 1-4 of the draft position paper because they are inappropriate; adopt a formal decision-analysis procedure for the 17 identified emplacement modes; revise App. F of the impact study to more accurately reflect current technology; consider designing the underground layout to take advantage of stress-relief techniques; consider eliminating reference to fuel assemblies <10 yr ''out-of-reactor''; model the temperature distribution, assuming that the repository is constructed in an infinitely large salt body; state that the results of creep analyses must be considered tentative until they can be validated by in situ measurements; and reevaluate the peak radial stresses on the waste package so that the calculated stress conditions more closely approximate expected in situ conditions

Preventive effect of ozone on the development of white spot lesions during multibracket appliance therapy.

Science.gov (United States)

Kronenberg, Otmar; Lussi, Adrian; Ruf, Sabine

2009-01-01

To test the null hypotheses: (1) there is no difference in the caries protective effect of ozone and Cervitec/Fluor Protector during multibracket (MB) appliance therapy, and (2) DIAGNOdent and quantitative light-induced fluorescence (QLF) are not superior to a visual evaluation of initial caries lesions. Twenty right-handed patients with a very poor oral hygiene who required full MB appliance therapy were analyzed during 26 months. In a split-mouth-design, the four quadrants of each patient were either treated with ozone, a combination of Cervitec and Fluor Protector, or served as untreated controls. The visible plaque index (VPI) and white spot formation were analyzed clinically. DIAGNOdent and QLF were used for a quantitative assessment of white spot formation. The average VPI in all four dental arch quadrants amounted to 55.6% and was independent of the preventive measure undertaken. In the quadrants treated with Cervitec/Fluor Protector, only 0.7% of the areas developed new, clinically visible white spots. This was significantly (P Fluor Protector during MB therapy was superior to ozone, and a visual evaluation of initial caries lesions was superior to both DIAGNOdent and QLF.

Methyl salicylate as a medium for radioassay of 36Cl using a liquid scintillation spectrometer

International Nuclear Information System (INIS)

Wiebe, L.I.; Ediss, C.

1976-01-01

Methyl salicylate (MS), a high refractive index liquid with wave-shifting properties, has been used as a Cherenkov radiation generating medium for the radioassay of 36 Cl by liquid scintillation (LS) spectrometer. Comparative experiments, using both a standard toluene-based LS fluor and toluene alone, for the measurement of 36 Cl were undertaken. The methyl salicylate medium was found to perform at an intermediate counting efficiency, near that for the LS fluor. In the presence of moderate amounts of nitromethane, the MS was less susceptible to chemical quenching effects than either the fluor or toluene. Counting efficiencies for 36 Cl in MS, toluene and toluene fluor respectively were 82.4, 28.4 and 100.3 percent with a Picker Liquimat 220 LS spectrometer, and 91.6, 54.9 and 100.0 percent with a Searle Mark III LS spectrometer. The addition of nitromethane (11.3 percent of final volume) reduced these efficiencies to 50.5, 10.0 and 15.4 percent, and to 58.8, 12.4 and 19.0 percent, respectively. The data are discussed in relation to observed changes in the pulse height spectra. Chemical quench correction by ESCR and by SCR methods is reported

Restoring public trust while tearing down site in rural Ohio

International Nuclear Information System (INIS)

Schneider, Jerry; Wagner, Jeffrey; Connell, Judy

2007-01-01

In the mid-1980's, the impact of three decades of uranium processing near rural Fernald, Ohio, 18 miles northwest of Cincinnati, became the centre of national public controversy. When a series of incidents at the uranium foundry brought to light the years of contamination to the environment and surrounding farmland communities, local citizens' groups united and demanded a role in determining the plans for cleaning up the site. One citizens' group, Fernald Residents for Environmental Safety and Health (FRESH), formed in 1984 following reports that nearly 300 pounds of enriched uranium oxide had been released from a dust-collector system, and three off-property wells south of the site were contaminated with uranium. For 22 years, FRESH monitored activities at Fernald and participated in the decision-making process with management and regulators. The job of FRESH ended on 19 January this year when the Fernald Site was declared clean of all nuclear contamination and open to public access. It marked the end of a remarkable turnaround in public confidence and trust that had attracted critical reports from around the world. When personnel from Fluor arrived in 1992, the management team thought it understood the issues and concerns of each stakeholder group, and was determined to implement the decommissioning scope of work aggressively, confident that stakeholders would agree with its plans. This approach resulted in strained relationships with opinion leaders during the early months of Fluor's contract. To forge better relationships, the U.S. Department of Energy (DOE) who owns the site, and Fluor embarked on three new strategies based on engaging citizens and interested stakeholder groups in the decision-making process. The first strategy was opening communication channels with site leadership, technical staff, and regulators. This strategy combined a strong public-information program with two-way communications between management and the community, soliciting and

Treatability Studies Used to Test for Exothermic Reactions of Plutonium Decontamination Chemicals

Energy Technology Data Exchange (ETDEWEB)

Ewalt, John R.; Minette, Michael J.; Hopkins, Andrea M.; Cooper, Thurman D.; Simiele, Connie J.; Scott, Paul A.; Scheele, Randall D.; Charboneau, Stacy L.

2005-08-07

Fluor Hanford is decommissioning the PFP at the Hanford Site and is considering using agressive chemicals to remove transuranium contaminants. As part of the evaluation of these methods, Fluor is considering the path for disposal and the thermal stability of the waste products from the decontamination process. This paper provides the results of our studies on cerium nitrate and RadPro(TM), a nitric acid based complexant.

Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models

Science.gov (United States)

Maawy, Ali A.; Hiroshima, Yukihiko; Kaushal, Sharmeela; Luiken, George A.; Hoffman, Robert M.; Bouvet, Michael

2013-12-01

The aim of this study was to evaluate a set of visible and near-infrared dyes conjugated to a tumor-specific chimeric antibody for high-resolution tumor imaging in orthotopic models of pancreatic cancer. BxPC-3 human pancreatic cancer was orthotopically implanted into pancreata of nude mice. Mice received a single intravenous injection of a chimeric anti-carcinoembryonic antigen antibody conjugated to one of the following fluorophores: 488-nm group (Alexa Fluor 488 or DyLight 488); 550-nm group (Alexa Fluor 555 or DyLight 550); 650-nm group (Alexa Fluor 660 or DyLight 650), or the 750-nm group (Alexa Fluor 750 or DyLight 755). After 24 h, the Olympus OV100 small-animal imaging system was used for noninvasive and intravital fluorescence imaging of mice. Dyes were compared with respect to depth of imaging, resolution, tumor-to-background ratio (TBR), photobleaching, and hemoglobin quenching. The longer wavelength dyes had increased depth of penetration and ability to detect the smallest tumor deposits and provided the highest TBRs, resistance to hemoglobin quenching, and specificity. The shorter wavelength dyes were more photostable. This study showed unique advantages of each dye for specific cancer imaging in a clinically relevant orthotopic model.

Environmental releases for calendar year 1996

International Nuclear Information System (INIS)

Greager, E.M.

1997-01-01

This report presents data on radioactive and nonradioactive materials released into the environment during calendar year 1996 from facilities and activities managed by the Fluor Daniel Hanford, Incorporated (formerly the Westinghouse Hanford Company) and Bechtel Hanford, Incorporated. Fluor Daniel Hanford, Incorporated provides effluent monitoring services for Bechtel Hanford, Incorporated, which includes release reporting. Both summary and detailed presentations of the environmental releases are provided. When appropriate, comparisons to data from previous years are made

Environmental releases for calendar year 1996

Energy Technology Data Exchange (ETDEWEB)

Greager, E.M.

1997-07-31

This report presents data on radioactive and nonradioactive materials released into the environment during calendar year 1996 from facilities and activities managed by the Fluor Daniel Hanford, Incorporated (formerly the Westinghouse Hanford Company) and Bechtel Hanford, Incorporated. Fluor Daniel Hanford, Incorporated provides effluent monitoring services for Bechtel Hanford, Incorporated, which includes release reporting. Both summary and detailed presentations of the environmental releases are provided. When appropriate, comparisons to data from previous years are made.

F{sup 19} relaxation in non-magnetic hexafluorides; Contribution a l'etude de la relaxation des fluors dans les hexafluorures non magnetiques

Energy Technology Data Exchange (ETDEWEB)

Rigny, P [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1967-12-01

The interesting properties of the fluorine magnetic resonance in the hexafluorides of molybdenum, tungsten and uranium, are very much due to large anisotropies of the chemical shift tensors. In the solid phases these anisotropies, the values of which are deduced from line shape studies, allow one to show that the molecules undergo hindered rotations about the metal atom. The temperature and frequency dependence of the fluorine longitudinal relaxation times shows that the relaxation is due to the molecular motion. The dynamical parameters of this motion are then deduced from the complete study of the fluorine relaxation in the rotating frame. In the liquid phases, the existence of anisotropies allows an estimation of the different contributions to the relaxation. In particular, the frequency and temperature dependence of the relaxation shows it to be dominated by the spin-rotation interaction. We have shown that the strength of this interaction can be deduced from the chemical shifts, and the angle through which the molecule rotates quasi-freely can be determined. In the hexafluorides, this angle is roughly one radian at 70 C, and with the help of this value, the friction coefficients which describe the intermolecular interactions are discussed. (author) [French] Les proprietes de la resonance magnetique des fluors dans les hexafluorures de molybdene, tungstene et uranium sont influencees par l'existence de deplacements chimiques tres anisotropes. Dans les phases solides, la valeur de cette anisotropie peut etre determinee par l'analyse des formes de raies et son existence permet de montrer que les molecules sont en rotation empechee autour de leur atome central. L'etude du temps de relaxation longitudinal en fonction de la temperature et de la frequence montre que la relaxation est due aux mouvements moleculaires, aux plus hautes temperatures. Les proprietes dynamiques du mouvement sont obtenues par l'etude complete de la relaxation spin-reseau dans le referentiel

The CHPRC Columbia River Protection Project Quality Assurance Project Plan

International Nuclear Information System (INIS)

Fix, N.J.

2008-01-01

Pacific Northwest National Laboratory researchers are working on the CHPRC Columbia River Protection Project (hereafter referred to as the Columbia River Project). This is a follow-on project, funded by CH2M Hill Plateau Remediation Company, LLC (CHPRC), to the Fluor Hanford, Inc. Columbia River Protection Project. The work scope consists of a number of CHPRC funded, related projects that are managed under a master project (project number 55109). All contract releases associated with the Fluor Hanford Columbia River Project (Fluor Hanford, Inc. Contract 27647) and the CHPRC Columbia River Project (Contract 36402) will be collected under this master project. Each project within the master project is authorized by a CHPRC contract release that contains the project-specific statement of work. This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by the Columbia River Project staff

The CHPRC Columbia River Protection Project Quality Assurance Project Plan

Energy Technology Data Exchange (ETDEWEB)

Fix, N. J.

2008-11-30

Pacific Northwest National Laboratory researchers are working on the CHPRC Columbia River Protection Project (hereafter referred to as the Columbia River Project). This is a follow-on project, funded by CH2M Hill Plateau Remediation Company, LLC (CHPRC), to the Fluor Hanford, Inc. Columbia River Protection Project. The work scope consists of a number of CHPRC funded, related projects that are managed under a master project (project number 55109). All contract releases associated with the Fluor Hanford Columbia River Project (Fluor Hanford, Inc. Contract 27647) and the CHPRC Columbia River Project (Contract 36402) will be collected under this master project. Each project within the master project is authorized by a CHPRC contract release that contains the project-specific statement of work. This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by the Columbia River Project staff.

Interest of a simple on-line screening registry for measuring ICU burden related to an influenza pandemic.

Science.gov (United States)

Richard, Jean-Christophe Marie; Pham, Tài; Brun-Buisson, Christian; Reignier, Jean; Mercat, Alain; Beduneau, Gaëtan; Régnier, Bernard; Mourvillier, Bruno; Guitton, Christophe; Castanier, Matthias; Combes, Alain; Le Tulzo, Yves; Brochard, Laurent

2012-07-09

The specific burden imposed on Intensive Care Units (ICUs) during the A/H1N1 influenza 2009 pandemic has been poorly explored. An on-line screening registry allowed a daily report of ICU beds occupancy rate by flu infected patients (Flu-OR) admitted in French ICUs. We conducted a prospective inception cohort study with results of an on-line screening registry designed for daily assessment of ICU burden. Among the 108 centers participating to the French H1N1 research network on mechanical ventilation (REVA) - French Society of Intensive Care (SRLF) registry, 69 ICUs belonging to seven large geographical areas voluntarily participated in a website screening-registry. The aim was to daily assess the ICU beds occupancy rate by influenza-infected and non-infected patients for at least three weeks. Three hundred ninety-one critically ill infected patients were enrolled in the cohort, representing a subset of 35% of the whole French 2009 pandemic cohort; 73% were mechanically ventilated, 13% required extra corporal membrane oxygenation (ECMO) and 22% died. The global Flu-OR in these ICUs was only 7.6%, but it exceeded a predefined 15% critical threshold in 32 ICUs for a total of 103 weeks. Flu-ORs were significantly higher in University than in non-University hospitals. The peak ICU burden was poorly predicted by observations obtained at the level of large geographical areas. The peak Flu-OR during the pandemic significantly exceeded a 15% critical threshold in almost half of the ICUs, with an uneven distribution with time, geographical areas and between University and non-University hospitals. An on-line assessment of Flu-OR via a simple dedicated registry may contribute to better match resources and needs.

Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas

Digital Repository Service at National Institute of Oceanography (India)

Clabby, C.; Goswami, U.; Flavin, F.; Wilkins, N.P.; Houghton, J.A.; Powell, R.

F2.18 (includ- ing pBGS8 vector) was labelled with fluor-12-dUTP and hybridized to the chromosome slides. The fluorescence signals were directly observed without additional immu- nological enhancement. No fluorescence signals were obtained when...-it-Fluor, Stratagene, La Jolla, CA, USA). Chromosomes were denatured at 72°C in 70% formamide/2 × SSC and the denatured labelled Cgl70 probe was added and hybridization performed at 37°C overnight. Post-hybridization washes were according to manufacturer...

High-speed videography combined with an x-ray image intensifier for dynamic radiography

International Nuclear Information System (INIS)

Bryant, L.E. Jr.

1983-01-01

The Spin Physics SP-2000 high-speed video system can be combined with an x-ray source, a dynamic event having internal (not directly visible) movement and an x-ray image intensifier to perform dynamic radiography. The cesium iodide input fluor and P-20 output fluor of the image intensifier have rapid decay to allow x-ray imaging up to 12,000 pictures per second. Applications of this technique include internal functioning of a compressor, turbulent-water action, and other mechanical actions

Time response measurements of LASL diagnostic detectors

International Nuclear Information System (INIS)

Hocker, L.P.

1970-07-01

The measurement and data analysis techniques developed under the Los Alamos Scientific Laboratory's detector improvement program were used to characterize the time and frequency response of selected LASL Compton, fluor-photodiode (NPD), and fluor-photomultiplier (NPM) diagnostic detectors. Data acquisition procedures and analysis methods presently in use are summarized, and detector time and frequency data obtained using the EG and G/AEC electron linear accelerator fast pulse (approximately 50 psec FWHM) as the incident radiation driving function are presented. (U.S.)

Progressively safer, cheaper demolition of Fernald

International Nuclear Information System (INIS)

Nichols, Robert; Pennington, Norman

2000-01-01

Fluor Fernald, Inc. has been progressively improving Decontamination and Dismantlement (D and D) at the Department of Energy's Fernald Environmental Management Project by applying new technologies and better methodologies to the work. Demolition issues existed in the past that necessitated new or improved solutions to maintain worker safety, protect the environment and accomplish the work in a cost effective manner. Lessons learned from D and D of 80 structures has led to a systematic approach, which can be implemented in various D and D arenas. When facility production was halted, hold-up material and process residues remained in the process piping and components. Over 500,000 pounds of material was removed by workers who completed the tasks two years ahead of schedule, $7 million under budget and with an excellent safety record. This success was the result of detailed planning and irdision of lessons learned as work progressed from facility to facility. Work sequences were developed that reduced airborne contamination. Demolition of structures has been performed at Fernald by carefully selected and qualified subcontractors. Asbestos and lead abatement, equipment, piping and conduit removal, and structural demolition have been completed to progressively higher performance specifications developed by Fluor Fernald based on lessons learned during execution. Safety continues to be the primary consideration in performing potentially hazardous work. Technologies such as hydraulic shears have been developed and used to keep workers away from danger. A new technology, ''Cool Suits,'' has been demonstrated to help prevent heat stress when anti-contamination clothing is required in elevated temperature working conditions. For tall structures, implosion technologies have been employed with progressively improved results, Several other new technologies have been evaluated by Fluor Fernald and applied by subcontractors. The improved technologies included the oxy-gas torch

Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

DEFF Research Database (Denmark)

Langel, Jennifer L; Smale, Laura; Esquiva, Gema

2015-01-01

projections of ipRGCs by examining the distribution of immunoreactive PACAP fibers in intact and enucleated animals. This revealed evidence that these cells project to the suprachiasmatic nucleus, lateral geniculate nucleus (LGN), pretectum, and superior colliculus. This distribution was confirmed...... with injections of cholera toxin subunit β coupled with Alexa Fluor 488 in one eye and Alexa Fluor 594 in the other, combined with IHC staining of PACAP. These studies also revealed that the ventral and dorsal LGN and the caudal olivary pretectal nucleus receive less innervation from ipRGCs than that reported...

Potensi “Khimelor” sebagai Tepung Komposit Tinggi Energi Tinggi Protein Berbasis Pangan Lokal (Health Potential of “Khimelor” as Composite Fluor Having Both High Energy and High Protein Level Based on Local Food

Directory of Open Access Journals (Sweden)

Laksmi Karunia Tanuwijaya

2016-07-01

The use of wheat flour as the basic ingredients of food products for high energy high protein diet is considerably high. Soybeans, mung beans, red leaf spinach and moringa leaf is local food sources of nutrients that are potential to be processed into a composite flour substituting wheat flour, which can be utilized for a variety of food products. The research was aimed to know the influence of proportion of soybeans, mung beans, red leaf spinach and moringa leaf (KhiMeLor on the quality of nutrition, protein and organoleptik quality of composite flour. This was experimental research with a complete random design. The treatment were several composition mixtures of wheat and composite flour, consisting of soybean, mung bean, red leaf spinach and moringa leaf P0 (100% : 0%; P1 (75% : 25%; P2 (50% : 50%; P3 (25% : 75%; P4 (0% : 100%. The content of carbohydrate, protein and fat was analyzed using proximate analysis. The quality of protein was examined from limiting amino acid and protein digestibility score. The sensory test was used to examine the costumer acceptance on sensory parameters. Statistical analysis used One Way Anova which  showed that substitution of  soy bean, mung bean,  moringaleaf and red spinach significantly (p=0,000 increases protein level. The limiting amino acid of P0,P1 and P2 was Lysine, but P3 and P4 was methionine. Protein digestibility of composite fluor was less than P0 (96%. The result of sensory evaluation showed that there was significant difference in aroma (p=0,000 and texture (p=0,029 which decreases as there is an increase of proportion composite flour. The conclusion was different proportion of soy bean, mung bean, moringa leaf and red spinach  influences the nutrition quality and sensory evaluation of composite flour. Keyword : composite flour, local food, KHiMelor

Technology qualification of an ambient pressure subsea cryogenic pipeline for offshore LNG loading and receiving terminals

Energy Technology Data Exchange (ETDEWEB)

Hussain, Afzal; Viteri, Martha; D' Angelo, Luis [Det Norske Veritas (DNV), Rio de Janeiro, RJ (Brazil); Prescott, Neal; Zhang, Jeff [Fluor Corporation, Irving, TX (Brazil)

2009-07-01

A project that deploys new technologies need to be confident that the technology can be implemented successfully and will perform reliably as designed. New technology is critical to industry, especially where such technology is a project enable without the existence of a backup solution, but also for projects where such technologies bring potential benefits such as technical, economic, schedule, and environmental improvements. DNV developed and has been implementing for many years a systematic, risk based technology qualification process as described in DNV RP-A203, qualification procedures for new technology. One of the major objectives of a formal technology qualification process is to ensure that risks are properly addressed. The DNV process includes several levels of technology qualification and review, starting with a statement of feasibility and concluding with a Certificate of Fitness for Service. Fluor Corporation (Fluor) has developed a new subsea cryogenic pipe-in-pipe configuration for offshore LNG loading and receiving terminals. The configuration uses a highly efficient thermal nano-porous insulation in the annular space between the inner and outer pipes. This material is kept in an ambient pressure environment, which is produced through sealing by metal bulkheads. The bulkheads transfer the contraction induced axial compression load on the inner cryogenic carrier pipe to the external jacket pipe. The resulting pipeline bundle is a structural element, which addresses the thermal contraction and expansion loads without the use of expansion bellows or ultra-low thermal contraction alloys. Fluor has followed the DNV technology qualification process to achieve the defined milestones therein which culminated in DNV issuing a certificate of fitness for service. Particular focus was put on the new aspects of the design. The certificate of fitness for service for the Fluor subsea LNG pipe technology provides project management with the confidence that this

[Recent findings in the etiopathogenesis of caries].

Science.gov (United States)

Farge, P

1998-10-01

Caries have three main interrelated etiological factors: 1) a bacteriological factor represented by cariogen oral bacteria, mainly Streptococcus mutans, which adhere to the dental surface and initiate the decalcification of the enamel by producing an acid environment through carbohydrate degradation; 2) the quality of the oral biofilm which is colonized more or less rapidly by bacteria after brushing, leading to an adhesive and aggressive dental plaque; 3) the quality of enamel which is reinforced by fluor ion, and of saliva. The early superficial phase of carie constitution is reversible through remineralisation from calcium, phosphate and fluor ions present in the oral cavity. In the absence of remineralisation the destructive and infectious process extends to the inner part of the tooth leading, to its progressive destruction. Prevention is based upon the following pathophysiological data, mainly: 1) family education on oral hygiene by regular brushing, and avoidance of foods or fluids containing sugars between meals; 2) fluoride intake starting at birth and being adapted to age, the prescription of fluoride supplementation, taking into account possible other sources of fluor (salt, water).

Use of flow scintillation analyzer combined with amino acid analyzer for measuring low-level radioactivity of tritium-labelled amino acids

CERN Document Server

Lukashina, E V; Fedoseev, V M; Ksenofontov, A L; Baratova, L A; Dobrov, E N

2002-01-01

Potential application of the Radiomatic 150TR Flow Scintillation Analyzer (Packard Instrument Co., USA) for measuring low radioactivity of tritium-labelled amino acids in eluate from the Amino Acid Analyzer 835 (Hitachi, Japan) was studied. Six scintillating cocktails were tested and the Hionic-Fluor and Ultima-Flo AP cocktails proved the most appropriate for flow measurement of radioactivity. Efficiency of tritium radioactivity recording under various conditions of analysis was determined. Under optimal conditions the lower detection limit for the Hionic-Fluor was 150, while for Ultima-Flo AP-100 decays/min in the peak of amino acid

Investigation of Chemical Durability Mechanisms and Structure of Fluoride Glasses.

Science.gov (United States)

1988-03-01

Compositions of fluorozirconate glasses (mol%) mnemonic ZrF 4 BaF 2 LaF 3 A1F 3 LiF source ZBLA 57 36 3 4 -- RADC* ZBLA-F 51.16 38.62 5.66 4.56 -- Le Verre ...Fluore4 ZBLAL 51.8 20.0 5.3 3.3 19.6 NRL * Rome Air Development Center, Hanscom AFB, MA (Dr. El Bayoumi) # Le Verre Fluore’ Co., LeMans, France " Naval...Maine, LeMans, France, (with partial support from the French Foreign Office), studied corrosion of UF4 -based fluorides. - Michel Le Toullec - 10/86

Comparison between non-dedicated positron emission tomography (NPET) with [F18] Fluor Deoxi glucose (FDG)and Radionuclide-labeled somatostatin analogues for the diagnostic purposes in the Differential Thyroid Cancer

International Nuclear Information System (INIS)

Escobar, E.I.; Giammarile, G.F.; Borson-Chazot, B.F.; Hasdi, H.Z.; Sassolas, S.G.

2002-01-01

Aim: Evaluation of sensibility in the diagnosis of metastasis or recurrence in Differential Thyroid Cancer in patients with elevated Thyroglobulin plasmatic level and a Negative Whole Body Iodine 131 scan. Material and Method: 23 patients with a differential thyroid cancer were included in this study (13 papillary cancer, 5 Follicular, 3 Hurtle cell and 1 insular), all of which had an elevated plasmatic level of thyroglobulin and negative scan performed after high doses of 131 Iodine. The results of Fluor deoxi glucose scan and Octreoscan were made independently of two observers and were confirmed afterwards with TAC, Ultrasound, histopathologic findings and the follow-up. The Octreoscan was performed under defrenation status. Results: The FDG scan has revealed 13/23 positive scan, the octreoscan 9/23 with a Sensibility of 6%, 39% and 60.8% respectively. In 17 studies the FDG scan and octreoscan concord. Besides, in 6 studies there was no concordance between FDG and octreoscan; 5 of which correspond to 5 false negative for octreoscan and 1 false negative of FDG. The level of plasmatic thyroglobulin was not an indicator of the presence of positive scans. Among false negative exams in FDG scan and ostreoscan, there were 8 cases of adenopathies cervical and 1 pulmonary metastasis. In the analysis of lesion site, we founded a mismatched between FDG and octreoscan: the FDG could identify 2 bone metastasis, 3 pulmonary metastasis, 2 cervical adenophaties, and 1 mediastinal focus where octreoscan was negative. Likewise, the octreoscan found 3 mediastinal metastasis correctly and 1 pulmonary metastasis where FDG was negative. Conclusion: In the search of a metastasis or recurrence for differential thyroid cancer, when the thyroglobulin is elevated and the whole body scan is negative, the FDG scan and octreoscan could have their indication. As a result, we can conclude that the FDG scan is a good tool and the study with octreoscan, on the contrary, shows a lower utilization

Kinetic study of the fluorination by fluorine of some uranium and plutonium compounds; Etude cinetique de la fluoration par le fluor de quelques composes de l'uranium et du plutonium

Energy Technology Data Exchange (ETDEWEB)

Vandenbussche, G [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

1964-12-15

The study of fluorination reactions of uranium and plutonium compounds with elementary fluorine, has been carried out using a thermogravimetric method. These reactions are heterogeneous ones, and of the following type: S(solid) + G{sub 1}(gas) - G{sub 2}(gas). The kinetics of these reactions correspond to a uniform attack of the entire surface of the sample. {alpha}: being the degree of completion of the reaction, k(rel): being the relative rate of penetration of the reaction interface, t: being the time, one have the relation: (1-{alpha}){sup 1/3} = 1 - k(rel)*t. The mechanism of the reaction varies according to the nature of the compound: 1) with uranium tetrafluoride and plutonium tetrafluoride, the reaction proceeds in a single step; 2) with uranium oxides, the reaction proceeds in two steps, uranium oxyfluoride being the intermediate compound; 3) with plutonium oxide, the reaction proceeds in two steps, plutonium tetrafluoride being the intermediate compound; and 4) with uranium trichloride, the mechanism is complex: chlorine trifluoride is formed. (author) [French] L'etude des reactions de fluoration par le fluor, de composes de l'uranium et du plutonium a ete faite par thermogravimetrie. Ce sont des reactions heterogenes du type: S(solide) + G{sub 1}(gaz) - G{sub 2}(gaz). La cinetique de ces reactions est celle correspondant a une attaque uniforme de toute la surface de l'echantillon. Si {alpha}: est le degre d'avancement de la reaction, k(rel): est la vitesse relative d'avancement d'un interface reactionnel, t: le temps. On a la relation: (1-{alpha}){sup 1/3} = 1-k(rel)*t. Le mecanisme de la reaction varie selon la nature du compose: 1) tetrafluorure d'uranium et tetrafluorure de plutonium, la reaction s'effectue en un seul stade; 2) Oxydes d'uranium: la reaction s'effectue en deux stades, l'oxyfluorure d'uranium est le compose intermediaire; 3) oxyde de plutonium, la reaction s'effectue en deux stades, la tetrafluorure de plutonium est le compose

Normal maintenance costs drop at Duke Power: Impact of Hurricane Hugo being assessed

International Nuclear Information System (INIS)

Anon.

1990-01-01

William S. Lee, chairman and president of Duke Power Company, said that a third-quarter earnings increase was primarily due to lower plant maintenance costs, but that figures aren't in yet on the damages related to Hurricane Hugo, which ripped through the Carolinas in late September. The severity of this storm is unprecedented in the company's history, he said. Lee also announced that Duke Power has entered into a new partnership with the Fluor Corporation, of Irvine, California. The two companies, operating under the name Duke/Fluor Daniel, will offer the power industry a single source of design, engineering, construction, and operation services for new and existing coal-fired plants

Preparation of plastic scintillator detectors and physicochemical parameter characterization

International Nuclear Information System (INIS)

Hamada, M.M.; Mesquita, C.H. de.

1988-10-01

The development of plastic scintillators for use in the nuclear radiation detection is described. The detectors were fabricated by the polymerization of styrene with organic fluors. The organic fluors used were PPO (1,4 diphenyl-oxazol) and POPOP 1,4-di-2-(5-phenyl-oxazolil) - benzene in proportions of 0,5 and 0,05% respectively. Physicochemical parameters related to the quality of this detector are investigated at this laboratory. The evaluation of its fluorescence characteristics, density, melting softening, refractive index, molecular weight, gamma and alpha spectrometry characteristics and finally the comparative pulse height analysis indicate that the plastic scintillator produced at this laboratory is comparable with others already described. (author)

Scintillator detector array

International Nuclear Information System (INIS)

Cusano, D.A.; Dibianca, F.A.

1981-01-01

This patent application relates to a scintillator detector array for use in computerized tomography and comprises a housing including a plurality of chambers, the said housing having a front wall transmissive to x-rays and side walls opaque to x-rays, such as of tungsten and tantalum, a liquid scintillation medium including a soluble fluor, the solvent for the fluor being disposed in the chambers. The solvent comprises either an intrinsically high Z solvent or a solvent which has dissolved therein a high Z compound e.g. iodo or bromonaphthalene; or toluene, xylene or trimethylbenzene with a lead or tin alkyl dissolved therein. Also disposed about the chambers are a plurality of photoelectric devices. (author)

Spectrographic determination of traces of halogens; Dosage de traces d'halogenes par la methode spectrographique

Energy Technology Data Exchange (ETDEWEB)

Melamed, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1961-07-01

Hollow cathode source is employed for determining traces of halogens (fluorine - chlorine) in the uranium oxide U{sub 3}O{sub 8} qualitatively, detection of at least 40 ppm of fluorine, as alkali fluoride and 125 ppm of chlorine, is possible. (author) [French] Un tube a decharge a cathode creuse a ete utilise pour la determination spectrographique des halogenes (fluor - chlore) presentes a l'etat de traces dans un oxyde d'uranium U{sub 3}O{sub 8}. On a pu deceler qualitativement des teneurs de 40 ppm de fluor sous forme de fluorures alcalins. En ce qui concerne le chlore, la plus faible teneur decelee a ete de 125 ppm. (auteur)

Preparation of plastic scintillation detectors and characterization of physico-chemical parameters

International Nuclear Information System (INIS)

Hamada, M.M.; Mesquita, C.H. de

1988-01-01

The development of plastic scintillators for using in the nuclear radiation detection is described. The detectors were fabricated by the polymerization of styrene with organic fluors. The organic fluors used were PPO (1,4 difenil-oxazol) and POPOP 1,4-di-2-(5-fenil-oxazolil) benzene in proportions of 0,5 and 0,05% respectively. Physical-chemistry parameters related to the quality of this detector are investigated at this laboratory. The evaluation of its fluorescency characteristics, density, melting softening, refractive index, molecular weight, gama and alfa espectrometry characteristics and finally the comparative pulse height analysis indicate that the plastic scintillator produced at this laboratory is comparable with others already described. (author) [pt

Radiochromic liquid solution

International Nuclear Information System (INIS)

Noakes, J.E.; Culp, R.A.

1983-01-01

A radiochromic solution which is sensitive to small dosages of ionizing and ultraviolet radiation is described. It consists of a solution of a leucocyanide dye in a clear polar solvent with enough organic acid added to make the solution at least slightly acidic and responds to radiation by permanently changing color. Up to one half of the solution by weight can be replaced by a second solution of an aromatic solvent and an organic fluor. Another modification of the invention is a solution of a leucocyanide dye in a clear polar solvent having an aromatic group, an organic fluor, and enough organic acid to make the solution at least slightly acidic. (author)

Integrity assessment report of tanks TK-101 and TK-102

Energy Technology Data Exchange (ETDEWEB)

MCSHANE, D.S.

1999-08-25

This Integrity Assessment Report (IAR) is prepared by Fluor Daniel Northwest (FDNW) for Waste Management Federal Services of Hanford, Inc., (WMH), the operations contractor; Fluor Daniel Hanford (FDH), the Hanford Site Manager; and the U. S. Department of Energy (DOE), the system owner. This IAR addresses the evaluation of Tanks 101 and 102 and other existing components located in the 219-S Waste Handling Facility. This report will be included in the Part B Permit for the 2226 Laboratory and is a portion of the integrity assessment of the overall 222-5 Laboratory radioactive liquid waste disposal system. This IAR is prepared in accordance with WAC 173-303, Dangerous Waste Regulations; Section 640(2), ''Assessment of Existing Tank Systems Integrity .''

Integrity assessment report of tanks TK-101 and TK-102

International Nuclear Information System (INIS)

MCSHANE, D.S.

1999-01-01

This Integrity Assessment Report (IAR) is prepared by Fluor Daniel Northwest (FDNW) for Waste Management Federal Services of Hanford, Inc., (WMH), the operations contractor; Fluor Daniel Hanford (FDH), the Hanford Site Manager; and the U. S. Department of Energy (DOE), the system owner. This IAR addresses the evaluation of Tanks 101 and 102 and other existing components located in the 219-S Waste Handling Facility. This report will be included in the Part B Permit for the 2226 Laboratory and is a portion of the integrity assessment of the overall 222-5 Laboratory radioactive liquid waste disposal system. This IAR is prepared in accordance with WAC 173-303, Dangerous Waste Regulations; Section 640(2), ''Assessment of Existing Tank Systems Integrity .''

Tolerancing requirements for remote handling at the Hanford vitrification project

International Nuclear Information System (INIS)

Keenan, R.M.; Bullis, R.E.; Van Katwijk, C.

1993-01-01

The Hanford Waste Vitrification Plant is being designed by Fluor Daniel, Inc. with WasteChem Corporation as Fluor Daniel's major subcontractor specializing in vitrification and remote system technologies. United Engineers and Constructors/Catalytic (UE ampersand C) will construct the plant. Westinghouse Hanford Company (WHC) is the Project Integration manager, manager and as the plant operator provides technical direction to the Architect/Engineer team (A/E) and constructor on behalf of the Department of Energy - Richland Field Office. The A/E has developed, in cooperation with UE ampersand C, WHC and DOE, a new and innovative approach to installations of the many remote nozzles and electrical connectors that must be installed to demanding tolerances. This paper summarizes the key elements of the HWVP approach

Effect of fluoride varnish on Streptococcus mutans counts in plaque of caries-free children using Dentocult SM strip mutans test: a randomized controlled triple blind study.

Science.gov (United States)

Jeevarathan, J; Deepti, A; Muthu, M S; Rathna Prabhu, V; Chamundeeswari, G S

2007-01-01

Dental caries is one of the most prevalent infectious diseases and although of multifactorial origin, Streptococcus mutans is considered the chief pathogen in its development. Fluoride is one of the most effective agents used for the reduction of dental caries apart from oral hygiene maintenance. The aim of this study was to estimate the counts of Streptococcus mutans and to evaluate the effect of Fluor Protector fluoride varnish on these counts in the plaque of caries-free children using Dentocult SM Strip Mutans. Thirty caries-free subjects were selected for the study based on the information obtained from a questionnaire and were randomly assigned to the control group consisting of ten subjects and the study group consisting of twenty subjects. Plaque samples were collected on the strips from the Dentocult SM kit and after incubation, the presence of Streptococcus mutans was evaluated using the manufacturer's chart. The study group was subjected to a Fluor Protector fluoride varnish application following which the samples were collected again after 24 hours. The average Streptococcus mutans counts in the primary dentition of caries-free children before and after the application of Fluor Protector fluoride varnish were 10(4)-10(5) colony forming units (CFU)/ml and <10(4) CFU/ml respectively. The results showed that the study group had a statistically significant reduction in the plaque Streptococcus mutans counts than the control group.

Quantification of osmotic water transport in vivo using fluorescent albumin.

Science.gov (United States)

Morelle, Johann; Sow, Amadou; Vertommen, Didier; Jamar, François; Rippe, Bengt; Devuyst, Olivier

2014-10-15

Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here, we describe the use of fluorescently labeled albumin as a reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as the gold standard. The pH-insensitive dye Alexa Fluor 555-albumin was applied to quantify osmotic water transport across the mouse peritoneal membrane resulting from modulating dialysate osmolality and genetic silencing of the water channel aquaporin-1 (AQP1). Quantification of osmotic water transport using Alexa Fluor 555-albumin closely correlated with direct volumetry and with estimations based on radioiodinated ((125)I) serum albumin (RISA). The low intraperitoneal pressure probably accounts for the negligible disappearance of the tracer from the peritoneal cavity in this model. Taken together, these data demonstrate the appropriateness of pH-insensitive Alexa Fluor 555-albumin as a practical and reliable intraperitoneal volume tracer to quantify osmotic water transport in vivo. Copyright © 2014 the American Physiological Society.

Quantification and characterization of enzymatically produced hyaluronan with fluorophore-assisted carbohydrate electrophoresis.

Science.gov (United States)

Kooy, Floor K; Ma, Muyuan; Beeftink, Hendrik H; Eggink, Gerrit; Tramper, Johannes; Boeriu, Carmen G

2009-01-15

Hyaluronan (HA) is a polysaccharide with high-potential medical applications, depending on the chain length and the chain length distribution. Special interest goes to homogeneous HA oligosaccharides, which can be enzymatically produced using Pasteurella multocida hyaluronan synthase (PmHAS). We have developed a sensitive, simple, and fast method, based on fluorophore-assisted carbohydrate electrophoresis (FACE), for characterization and quantification of polymerization products. A chromatographic pure fluorescent template was synthesized from HA tetrasaccharide (HA4) and 2-aminobenzoic acid. HA4-fluor and HA4 were used as template for PmHAS-mediated polymerization of nucleotide sugars. All products, fluorescent and nonfluorescent, were analyzed with gel electrophoresis and quantified using lane densitometry. Comparison of HA4- and HA4-fluor-derived polymers showed that the fluorophore did not negatively influence the PmHAS-mediated polymerization. Only even-numbered oligosaccharide products were observed using HA4-fluor or HA4 as template. The fluorophore intensity was linearly related to its concentration, and the limit of detection was determined to be 7.4pmol per product band. With this assay, we can now differentiate oligosaccharides of size range DP2 (degree of polymerization 2) to approximately DP400, monitor the progress of polymerization reactions, and measure subtle differences in polymerization rate. Quantifying polymerization products enables us to study the influence of experimental conditions on HA synthesis.

Late magmatic controls on the origin of schorlitic and foititic tourmalines from late-Variscan peraluminous granites of the Arbus pluton (SW Sardinia, Italy): Crystal-chemical study and petrological constraints

Science.gov (United States)

Bosi, Ferdinando; Naitza, Stefano; Skogby, Henrik; Secchi, Francesco; Conte, Aida M.; Cuccuru, Stefano; Hålenius, Ulf; De La Rosa, Nathaly; Kristiansson, Per; Charlotta Nilsson, E. J.; Ros, Linus; Andreozzi, Giovanni B.

2018-05-01

Tourmalines from the late-Variscan Arbus pluton (SW Sardinia) and its metamorphic aureole were structurally and chemically characterized by single-crystal X-ray diffraction, electron and nuclear microprobe analysis, Mössbauer, infrared and optical absorption spectroscopy, to elucidate their origin and relationships with the magmatic evolution during the pluton cooling stages. The Arbus pluton represents a peculiar shallow magmatic system, characterized by sekaninaite (Fe-cordierite)-bearing peraluminous granitoids, linked via AFC processes to gabbroic mantle-derived magmas. The Fe2+-Al-dominant tourmalines occur in: a) pegmatitic layers and pods, as prismatic crystals; b) greisenized rocks and spotted granophyric dikes, as clots or nests of fine-grained crystals in small miaroles locally forming orbicules; c) pegmatitic veins and pods close to the contacts within the metamorphic aureole. Structural formulae indicate that tourmaline in pegmatitic layers is schorl, whereas in greisenized rocks it ranges from schorl to fluor-schorl. Tourmalines in thermometamorphosed contact aureole are schorl, foitite and Mg-rich oxy-schorl. The main substitution is Na + Fe2+ ↔ □ + Al, which relates schorl to foitite. The homovalent substitution (OH) ↔ F at the O1 crystallographic site relates schorl to fluor-schorl, while the heterovalent substitution Fe2+ + (OH, F) ↔ Al + O relates schorl/fluor-schorl to oxy-schorl. Tourmaline crystallization in the Arbus pluton was promoted by volatile (B, F and H2O) enrichment, low oxygen fugacity and Fe2+ activity. The mineralogical evolutive trend is driven by decreasing temperature, as follows: sekaninaite + quartz → schorl + quartz → fluor-schorl + quartz → foitite + quartz. The schorl → foitite evolution represents a distinct trend towards (Al + □) increase and unit-cell volume decrease. These trends are typical of granitic magmas and consistent with Li-poor granitic melts, as supported by the absence of elbaite and other

The efficiency of different disinfecting agents in inactivating microorganisms detected in natural and treated waters; Eficiencia de diferentes agentes desinfectantes en la inactivacion de microorganismos detectados en aguas naturales y tratadas

Energy Technology Data Exchange (ETDEWEB)

Perez Recuerda, R.; Sanchez, J.M.; Borrego, J.J.

1998-12-01

The efficiency of microbial inactivation and sublethal injury of six disinfectants (chlorine, chloramines, uV-light, potassium permanganate, fluor and ozone) applied at different dose on several bacterial strains, yeast and viruses has been studied comparatively. Disinfectant effect was higher on Gramnegative bacteria (Salmonella, Pseudomonas, Escherichia and Klebsiella) than on Gram-positive (Clostridium, Enterococcus and Stanphylococcus); although the least inactivation effect was obtained on the MS-2 bacteriophage. The global efficiency ranking of the disinfectants assayed to produce the microbial inactivation was as follows; ozone>chlorine>UV-light>chloramines>permanganate>fluor. On the other hand, on Escherichia coli and Pseudomonas aerugionosa were observed the highest sublethal injuries provokes by the disinfectants and dose assayed. Therefore, these microorganisms are the main candidates to regrow and to form biofilm in drinking water distribution systems. 34 refs. (Author)

Examination of vegetation around a nuclear plant emitting gaseous fluorides in order to detect fluorine pollution; Utilisation des vegetaux pour detecter la pollution fluoree autour d'une usine susceptible d'emettre des effluents gazeux fluores

Energy Technology Data Exchange (ETDEWEB)

Teulon, Francoise; Bonnaventure, J. P. [Commissariat a l' energie atomique et aux energies alternatives - CEA, Centre de Pierrelatte, Section de Protection contre les Radiations (France)

1971-08-15

Fluorine pollution (chronic or occasional) around a plant rejecting gaseous fluoride effluents can be detected from vegetation samples by chemical analysis. Systematic monitoring allows the effects and gravity of the pollution to be estimated. The analytical method used consists of a double distillation (in phosphoric acid and perchloric acid) followed by a spectro-colorimetric analysis (alizarine-complexon-lanthane). This method of control allows both the efficiency of the trapping installations and also the appearance of effluents at unexpected places to be checked, In the event of an accident it is possible to determine the advisability of prohibiting the consumption of locally grown produce by humans or fodder by cattle. Research conducted in order to determine the relation between visible, damage to certain vegetables (tomatoes, haricot beans and sorghum) and their fluorine contents demonstrated that such a relation appears above all at the level of the leaves; chemical analysis may thus be used to confirm or reject information obtained on the basis of visual evidence [French] La detection d'une pollution fluoree (chronique ou accidentelle) autour d'une usine susceptible d'emettre des effluents gazeux fluores peut etre avantageusement realisee par un reseau de prelevements vegetaux suivis de dosages chimiques. Une surveillance systematique permet une evaluation des consequences et du degre de gravite de la pollution. La methode d'analyse consiste en une double distillation (dans l'acide phosphorique et l'acide perchlorique) suivie d'une spectrocolorimetrie (alizarine-complexon-lanthane). Ce mode de controle permet non seulement de verifier si les installations de piegeage sont efficaces mais egalement de localiser des points d'emission imprevus. En cas d'accident, on peut egalement juger de l'opportunite d'interdire la consommation des legumes par les habitants ou du fourrage par le betail des environs. Enfin, des etudes experimentales ont ete realisees pour

Effect of fluoride varnish on Streptococcus mutans counts in plaque of caries-free children using dentocult SM strip mutans test: A randomized controlled triple blind study

Directory of Open Access Journals (Sweden)

Jeevarathan J

2007-01-01

Full Text Available Dental caries is one of the most prevalent infectious diseases and although of multifactorial origin, Streptococcus mutans is considered the chief pathogen in its development. Fluoride is one of the most effective agents used for the reduction of dental caries apart from oral hygiene maintenance. Aims: The aim of this study was to estimate the counts of Streptococcus mutans and to evaluate the effect of Fluor Protector fluoride varnish on these counts in the plaque of caries-free children using Dentocult SM Strip Mutans. Materials and Methods: Thirty caries-free subjects were selected for the study based on the information obtained from a questionnaire and were randomly assigned to the control group consisting of ten subjects and the study group consisting of twenty subjects. Plaque samples were collected on the strips from the Dentocult SM kit and after incubation, the presence of Streptococcus mutans was evaluated using the manufacturer′s chart. The study group was subjected to a Fluor Protector fluoride varnish application following which the samples were collected again after 24 hours. Results: The average Streptococcus mutan s counts in the primary dentition of caries-free children before and after the application of Fluor Protector fluoride varnish were 10 4 -10 5 colony forming units (CFU/ml and < 10 4 CFU/ml respectively. Conclusion: The results showed that the study group had a statistically significant reduction in the plaque Streptococcus mutans counts than the control group.

Nanoscale control of Ag nanostructures for plasmonic fluorescence enhancement of near-infrared dyes

KAUST Repository

Xie, Fang; Pang, Jing S.; Centeno, Anthony; Ryan, Mary P.; Riley, D. Jason; Alford, Neil M.

2013-01-01

of increasing the sensitivity of protein detection in clinical applications. We report the use of tunable plasmonic silver nanostructures for the fluorescence enhancement of a near-infrared (NIR) dye (Alexa Fluor 790). Extensive fluorescence enhancement of ∼2

LEADING WITH LEADING INDICATORS

International Nuclear Information System (INIS)

PREVETTE, S.S.

2005-01-01

This paper documents Fluor Hanford's use of Leading Indicators, management leadership, and statistical methodology in order to improve safe performance of work. By applying these methods, Fluor Hanford achieved a significant reduction in injury rates in 2003 and 2004, and the improvement continues today. The integration of data, leadership, and teamwork pays off with improved safety performance and credibility with the customer. The use of Statistical Process Control, Pareto Charts, and Systems Thinking and their effect on management decisions and employee involvement are discussed. Included are practical examples of choosing leading indicators. A statistically based color coded dashboard presentation system methodology is provided. These tools, management theories and methods, coupled with involved leadership and employee efforts, directly led to significant improvements in worker safety and health, and environmental protection and restoration at one of the nation's largest nuclear cleanup sites

SAFETY STUDIES TO MEASURE EXOTHERMIC REACTIONS OF SPENT PLUTONIUM DECONTAMINATION CHEMICALS USING WET AND DRY DECONTAMINATION METHODS

International Nuclear Information System (INIS)

HOPKINS, A.M.; JACKSON, G.W.; MINETTE, M.; EWALT, J.; COOPER, T.; SCOTT, P.; JONES, S.; SCHEELEY, R.

2005-01-01

The Plutonium Finishing Plant (PFP) at the Hanford site in Eastern Washington is currently being decommissioned by Fluor Hanford. Chemicals being considered for dccontamination of gloveboxes in PFP include cerium (IV) nitrate in a nitric acid solution, and proprietary commercial solutions that include acids and sequestering agents. Aggressive chemicals are commonly used to remove transuranic contaminants from process equipment to allow disposal of the equipment as low level waste. Fluor's decontamination procedure involves application of chemical solutions as a spray on the contaminated surfaces, followed by a wipe-down with rags. Alternatively, a process of applying oxidizing Ce IV ions contained in a gel matrix and vacuuming a dry gel material is being evaluated. These processes effectively transfer the transuranic materials to rags or a gel matrix which is then packaged as TRU waste and disposed

Tolerancing requirements for remote handling at the Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Van Katwijk, C.; Keenan, R.M.; Bullis, R.E.

1993-01-01

The Hanford Waste Vitrification Plant (HWVP) is being designed by Fluor Daniel, Inc. with Waste Chem Corporation as Fluor Daniel, Inc.'s major subcontractor specializing in vitrification and remote system technologies. United Engineers and Constructors (UE ampersand C)/Catalytic (UCAT) will construct the plant. Westinghouse Hanford Company is the Project Integration manager and Business manager, and as the plant operator it provides technical direction to the Architect/ Engineer team (A/E) and constructor on behalf of the US Department of Energy - Richland Field Office. The A/E has developed, in cooperation with UE ampersand C, Westinghouse Hanford Company, and the US Department of Energy, a new and innovative approach to installations of the many remote nozzles and electrical connectors that must be installed to demanding tolerances. This paper summarizes the key elements of the HWVP approach

Decay Of Bacterial Pathogen, Fecal Indicators, And Real-Time Quantitative PCR Genetic Markers In Manure Amended Soils

Science.gov (United States)

This study examined persistence and decay of bacterial pathogens, fecal indicator bacteria, and emerging real-time quantitative PCR (qPCR) genetic markers for rapid detection of fecal pollution in manre-amended agricultural soils. Known concentrations of transformed green fluore...

CSER 99-002: CSER for unrestricted moderation of sludge material with two-boat operations in gloveboxes HC-21A and HC-21C

International Nuclear Information System (INIS)

LAN, J.S.

1999-01-01

This Criticality Safety Evaluation Report was prepared by Fluor Daniel Northwest under contract to BWHC. This document establishes the criticality safety parameters for unrestricted moderation of Sludge material with two-boat operations in gloveboxes HC-21A and HC-21C

Environmental Management Performance Report July 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-07-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-RL) a monthly summary of the Project Hanford Management Contractor's (PHMC) Environmental Management (EM) performance by Fluor Hanford (FH) and its subcont.

Early postnatal exposure to lithium in vitro induces changes in ...

Indian Academy of Sciences (India)

treated cultures. Rate of dye loss from vesicles is influenced by the extent of .... Recyc-II-loaded boutons in lithium-treated cultures show significant enhancement in fluores- cence and ... replaced with media containing either 2 μM H-89 (for.

FFTF Authorization Agreement

International Nuclear Information System (INIS)

DAUTEL, W.A.

2000-01-01

The purpose of the Authorization Agreement is to serve as a mechanism whereby the U.S. Department of Energy, Richland Operations Office (RL) and Fluor Hanford (FH) jointly clarify and agree to key conditions for conducting work safely and efficiently

A novel analytical method for in vivo phosphate tracking (Corrigendum in FEBS Letters, 2007, 581 p. 579)

DEFF Research Database (Denmark)

Gu, H.; Lalonde, S.; Okumoto, S.

2006-01-01

Genetically-encoded fluorescence resonance energy transfer (FRET) sensors for phosphate (Pi) (FLIPPi) were engineered by fusing a predicted Synechococcus phosphate-binding protein (PiBP) to eCFP and Venus. Purified fluorescent indicator protein for inorganic phosphate (FLIPPi), in which the fluor...

IN-SITU TRITIUM BETA DETECTOR

International Nuclear Information System (INIS)

Berthold, J.W.; Jeffers, L.A.

1998-01-01

The objectives of this three-phase project were to design, develop, and demonstrate a monitoring system capable of detecting and quantifying tritium in situ in ground and surface waters, and in water from effluent lines prior to discharge into public waterways. The tritium detection system design is based on measurement of the low energy beta radiation from the radioactive decay of tritium using a special form of scintillating optical fiber directly in contact with the water to be measured. The system consists of the immersible sensor module containing the optical fiber, and an electronics package, connected by an umbilical cable. The system can be permanently installed for routine water monitoring in wells or process or effluent lines, or can be moved from one location to another for survey use. The electronics will read out tritium activity directly in units of pico Curies per liter, with straightforward calibration. In Phase 1 of the project, we characterized the sensitivity of fluor-doped plastic optical fiber to tritium beta radiation. In addition, we characterized the performance of photomultiplier tubes needed for the system. In parallel with this work, we defined the functional requirements, target specifications, and system configuration for an in situ tritium beta detector that would use the fluor-doped fibers as primary sensors of tritium concentration in water. The major conclusions from the characterization work are: A polystyrene optical fiber with fluor dopant concentration of 2% gave best performance. This fiber had the highest dopant concentration of any fibers tested. Stability may be a problem. The fibers exposed to a 22-day soak in 120 F water experienced a 10x reduction in sensitivity. It is not known whether this was due to the build up of a deposit (a potentially reversible effect) or an irreversible process such as leaching of the scintillating dye. Based on the results achieved, it is premature to initiate Phase 2 and commit to a prototype

IN-SITU TRITIUM BETA DETECTOR

Energy Technology Data Exchange (ETDEWEB)

J.W. Berthold; L.A. Jeffers

1998-04-15

The objectives of this three-phase project were to design, develop, and demonstrate a monitoring system capable of detecting and quantifying tritium in situ in ground and surface waters, and in water from effluent lines prior to discharge into public waterways. The tritium detection system design is based on measurement of the low energy beta radiation from the radioactive decay of tritium using a special form of scintillating optical fiber directly in contact with the water to be measured. The system consists of the immersible sensor module containing the optical fiber, and an electronics package, connected by an umbilical cable. The system can be permanently installed for routine water monitoring in wells or process or effluent lines, or can be moved from one location to another for survey use. The electronics will read out tritium activity directly in units of pico Curies per liter, with straightforward calibration. In Phase 1 of the project, we characterized the sensitivity of fluor-doped plastic optical fiber to tritium beta radiation. In addition, we characterized the performance of photomultiplier tubes needed for the system. In parallel with this work, we defined the functional requirements, target specifications, and system configuration for an in situ tritium beta detector that would use the fluor-doped fibers as primary sensors of tritium concentration in water. The major conclusions from the characterization work are: A polystyrene optical fiber with fluor dopant concentration of 2% gave best performance. This fiber had the highest dopant concentration of any fibers tested. Stability may be a problem. The fibers exposed to a 22-day soak in 120 F water experienced a 10x reduction in sensitivity. It is not known whether this was due to the build up of a deposit (a potentially reversible effect) or an irreversible process such as leaching of the scintillating dye. Based on the results achieved, it is premature to initiate Phase 2 and commit to a prototype

77 FR 50102 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

Science.gov (United States)

2012-08-20

... support the export of U.S. services and equipment to Saudi Arabia. Brief non-proprietary description of... Incorporated; Jacobs Engineering Group Incorporated; Foster Wheeler AG; Fluor Corporation. Obligor: The obligor... Being Exported The items being exported are design work, construction services, technology licenses...

LESSONS LEARNED IN OPERATING THE HOSE-IN-HOSE SYSTEM FOR TRANSFSERRING SLUDGE AT HANFORD'S K BASINS

International Nuclear Information System (INIS)

PERES MW

2008-01-01

In May 2007, the Department of Energy and the Fluor Hanford K Basin Closure Project completed transferring sludge from the K East Basin to new containers in the K West Basin using a Hose-in-Hose system. This project presented a number of complex and unique technical, operational, and management challenges that had to be resolved to complete the required transfers and satisfy project milestones. The project team (including DOE; regulators; and Fluor management, operations, maintenance, engineering and all other support organizations) found innovative solutions to each challenge. This paper records lessons learned during the operational phase of the sludge transfer via the Hose-In-Hose system. The subject is limited to the operational phase and does not cover design, development, testing or turnover. A discussion of the situation or problem encountered is provided, along with the lesson learned as applicable to a future program or project

Clinical evaluation of high dose rate intra-cavitary irradiation for treatment of uterine cervical cancer, combined with pepleomycin suppository in uterine cavity

International Nuclear Information System (INIS)

Yamanashi, Shunji; Abe, Tatsuyuki; Mochizuki, Sachio; Murakami, Yoshitaka; Iida, Nobuhisa.

1990-01-01

By means of re-irradiation using pepleomycin suppository in uterine cavity, we attained local control for one patient who had local recurrence in uterine cavity and suffered from uterine fluor in which viable cancer cells were confirmed. We were enlightened by this therapeutic experience, so we attempted combination therapy using pepleomycin suppositories to supplement intra-cavitary irradiation, for the 11 selected patients who were suffering from uterine fluor. We investigated the treatment results in 7 patients of stage III out of 11 patients (of all stages), in comparison with 13 patients of stage III who were treated by irradiation alone. Consequently, these treatment results were approximately equivalent, and the incidence of sigmoid complications could be decreased. Side effects which were followed by the combination therapy were not serious, and so we believe that pepleomycin suppository is a simple method and valuable to supplement radiation therapy of uterine cervical cancer. (author)

Clinical evaluation of high dose rate intra-cavitary irradiation for treatment of uterine cervical cancer, combined with pepleomycin suppository in uterine cavity

Energy Technology Data Exchange (ETDEWEB)

Yamanashi, Shunji; Abe, Tatsuyuki; Mochizuki, Sachio (Jikei Univ., Tokyo (Japan). School of Medicine); Murakami, Yoshitaka; Iida, Nobuhisa

1990-02-01

By means of re-irradiation using pepleomycin suppository in uterine cavity, we attained local control for one patient who had local recurrence in uterine cavity and suffered from uterine fluor in which viable cancer cells were confirmed. We were enlightened by this therapeutic experience, so we attempted combination therapy using pepleomycin suppositories to supplement intra-cavitary irradiation, for the 11 selected patients who were suffering from uterine fluor. We investigated the treatment results in 7 patients of stage III out of 11 patients (of all stages), in comparison with 13 patients of stage III who were treated by irradiation alone. Consequently, these treatment results were approximately equivalent, and the incidence of sigmoid complications could be decreased. Side effects which were followed by the combination therapy were not serious, and so we believe that pepleomycin suppository is a simple method and valuable to supplement radiation therapy of uterine cervical cancer. (author).

Installation and routing of critical embedments at the Hanford Waste Vitrification Plant

International Nuclear Information System (INIS)

Van Katwijk, C.; Keenan, R.M.; Watts, C.

1993-01-01

The Hanford Waste Vitrification Plant (HWVP) is being designed by Fluor Daniel. Waste Chem Corporation is providing specialized expertise as Fluor Daniel's major subcontractor for vitrification and remote systems technologies. Westinghouse Hanford Company (Westinghouse Hanford) is the Project Integration manager and Business manager, and as the plant operator it provides technical direction to the Architect/Engineer team and constructor on behalf of the US Department of Energy, Richland Field Office. The Hot Cell portion of HWVP Vitrification Building contains very congested piping systems in the walls that penetrate in to the cells to nozzles for remote piping jumper assemblies. These nozzles require very tight tolerances to ensure a leak-tight fit to the jumpers. An approach has been developed that minimizes the time and expense of installing these nozzles in the wall to tight construction tolerances. This approach is called the Ganged Embed Plate (GEP) design

Polysiloxane scintillator composition

Science.gov (United States)

Walker, J.K.

1992-05-05

A plastic scintillator useful for detecting ionizing radiation comprising a matrix which comprises an optically transparent polysiloxane having incorporated therein at least one ionizing radiation-hard fluor capable of converting electromagnetic energy produced in the polysiloxane upon absorption of ionizing radiation to detectable light.

HPLC-HRMS Quantification of the Ichthyotoxin Karmitoxin from Karlodinium armiger

DEFF Research Database (Denmark)

Andersen, Aaron John Christian; Soman De Medeiros, Lívia; Binzer, Sofie Bjørnholt

2017-01-01

L). The standard was quantified by fluorescent derivatisation using Waters AccQ-Fluor reagent and derivatised fumonisin B₁ and fumonisin B₂ as standards, as each contain a primary amine. Various sample preparation methods for whole culture samples were assessed, including six different solid phase...

Penetration of varnishes into demineralized root dentine in vitro

NARCIS (Netherlands)

Arends, J; Duschner, H; Ruben, JL

1997-01-01

In this paper the penetration of three different varnishes employed in caries prevention (Duraphat(R), Fluor Protector(R) and Cervitec(R)) into demineralized dentine is quantified using confocal laser scanning microscopy. The results show that the varnish penetration into lesions about 85 mu m in

Metabolic Mapping of Breast Cancer with Multiphoton Spectral and Lifetime Imaging

Science.gov (United States)

2007-03-01

2002. Spectrally resolved fluorescence lifetime imaging microscopy. Appl. Spec- trosc. 56 :155-166. 38. Becker, W., A. Bergmann, E. Haustein , Z...photon fluores- cence lifetime imaging microscopy of macrophage-mediated antigen processing. J. Microsc. 185 :339-353. 45. Lin, H.J., P. Herman , and

Comparison of two bond strength testing methodologies for bilayered all-ceramics

NARCIS (Netherlands)

Dundar, Mine; Ozcan, Mutlu; Gokce, Bulent; Comlekoglu, Erhan; Leite, Fabiola; Valandro, Luiz Felipe

Objectives. This study compared the shear bond strength (SBS) and microtensile (MTBS) testing methodologies for core and veneering ceramics in four types of all-ceramic systems. Methods. Four different ceramic veneer/core combinations, three of which were feldspathic and the other a fluor-apatite to

Technical Note: Characteristics and Sorting of White Food Corn Contaminated with Mycotoxins

Science.gov (United States)

Samples of white corn grown in southern Texas were collected for characterization and sorting of kernels containing mycotoxins. Kernels were grouped into one of six symptom categories depending on the degree of visible discoloration and bright green-yellow fluorescence (BGYF) or bright orange fluor...

Effect of a chlorhexidine/thymol and a fluoride varnish on caries development in erupting permanent molars

DEFF Research Database (Denmark)

Flamee, S; Gizani, S; Caroni, C

2015-01-01

(Fluor Protector; FV group). The duration of the study was 2 years. The primary endpoint was caries incidence (initial and cavitated) in the erupting molars and the secondary outcome was salivary mutans streptococci (MS) counts. RESULTS: The groups were balanced with respect to socio-economy, oral...

Cytology of infection of apple leaves by Diplocarpon mali

Science.gov (United States)

Diplocarpon mali, the causal agent of Marssonina leaf blotch of apple, causes severe defoliation during the growing season. Little information is available on the mode of infection and infection process. In this study, the infection strategies of D. mali in apple leaves were investigated using fluor...

A Fluorescent Assay for Plant Caffeic Acid O-methyltransferases

Science.gov (United States)

We have developed a facile, sensitive and continuous assay to measure the activities of plant COMTs using s-adenosyl homocysteine hydrolase as a coupling enzyme and and adeonsine a thiol-specific fluor, Thioglo1, as the detecting reagent. This assay was validated using recombinant sorghum COMT (BMR-...

Quantitative estimation of the fluorescent parameters for crop leaves with the Bayesian inversion

Science.gov (United States)

In this study, the fluorescent parameters of crop leaves were retrieved from the leaf hyperspectral measurements by inverting the FluorMODleaf model, which is a leaf-level fluorescence model that is based on the widely used and validated PROSPECT (leaf optical properties) model and can simulate the ...

Mobile Work Platform - A Fluor Fernald innovative dismantlement technology

Energy Technology Data Exchange (ETDEWEB)

Mark S. Peters; Paul R. Cromer; Robert Danner

2000-06-16

The Department of Energy's (DOE) Office of Science and Technology Decontamination and Decommissioning (D&D) Focus Area, led by the National Energy Technology Laboratory, has been charged with finding new and innovative D&D technologies and then validating through field demonstration that the technologies are safer, faster and/or more cost-effective. The D&D Focus Area's approach to verifying the benefits of the improved D&D technologies is to use them at DOE sites in large-scale demonstration and deployment (LSDD) projects. The DOE's Fernald Environmental Management Project (FEMP), near Cincinnati Ohio, was host for a LSDD Project overseen by the D&D Focus Area. The FEMP was formerly engaged in the production of high quality uranium metal; and is now currently undergoing active environmental restoration, including removal of major process facilities. As observed during the D&D of Fernald's Plant 1, the baseline method for removing piping required laborers to work above the floor on ladders, scaffolding, ardor man-lifts with hand-held power tools. The pipe must first be rigged to prevent falling when cut. After cutting, the pipe is manually lowered to the ground and placed in a storage/disposal container. The Mobile Work Platform (MWP) consists of a mobile chassis, telescoping arm and a dual crimper/shear ''end-effecter''. It has the capability to grab and hold a pipe, crimp and shear the pipe (up to a ten-foot section) on either side of where it is being held and then lower and place the pipe section into a storage/disposal container. The MWP can crimp/shear up to a 6-inch diameter, schedule 401, carbon steel pipe. A single operator using a radio remote control operates the MWP. The paper will describe the results (productivity, safety advantages and lessons learned) during the Mobile Work Platform demonstration at Fernald.

Mobile Work Platform - A Fluor Fernald innovative dismantlement technology

International Nuclear Information System (INIS)

Peters, Mark S.; Cromer, Paul R.; Danner, Robert

2000-01-01

The Department of Energy's (DOE) Office of Science and Technology Decontamination and Decommissioning (D andD) Focus Area, led by the National Energy Technology Laboratory, has been charged with finding new and innovative D and D technologies and then validating through field demonstration that the technologies are safer, faster and/or more cost-effective. The D and D Focus Area's approach to verifying the benefits of the improved D and D technologies is to use them at DOE sites in large-scale demonstration and deployment (LSDD) projects. The DOE's Fernald Environmental Management Project (FEMP), near Cincinnati Ohio, was host for a LSDD Project overseen by the D and D Focus Area. The FEMP was formerly engaged in the production of high quality uranium metal; and is now currently undergoing active environmental restoration, including removal of major process facilities. As observed during the D and D of Fernald's Plant 1, the baseline method for removing piping required laborers to work above the floor on ladders, scaffolding, ardor man-lifts with hand-held power tools. The pipe must first be rigged to prevent falling when cut. After cutting, the pipe is manually lowered to the ground and placed in a storage/disposal container. The Mobile Work Platform (MWP) consists of a mobile chassis, telescoping arm and a dual crimper/shear ''end-effecter''. It has the capability to grab and hold a pipe, crimp and shear the pipe (up to a ten-foot section) on either side of where it is being held and then lower and place the pipe section into a storage/disposal container. The MWP can crimp/shear up to a 6-inch diameter, schedule 401, carbon steel pipe. A single operator using a radio remote control operates the MWP. The paper will describe the results (productivity, safety advantages and lessons learned) during the Mobile Work Platform demonstration at Fernald

Fis protein induced λF-DNA bending observed by single-pair fluorescence resonance energy transfer

Science.gov (United States)

Chi-Cheng, Fu; Wunshain, Fann; Yuan Hanna, S.

2006-03-01

Fis, a site-specific DNA binding protein, regulates many biological processes including recombination, transcription, and replication in E.coli. Fis induced DNA bending plays an important role in regulating these functions and bending angle range from ˜50 to 95 dependent on the DNA sequence. For instance, the average bending angle of λF-DNA (26 bp, 8.8nm long, contained λF binding site on the center) measured by gel mobility shift assays was ˜ 94 . But the traditional method cannot provide information about the dynamics and the angle distribution. In this study, λF-DNA was labeled with donor (Alexa Fluor 546) and acceptor (Alexa Fluor 647) dyes on its two 5' ends and the donor-acceptor distances were measured using single-pair fluorescence resonance energy transfer (sp-FRET) with and without the present of Fis protein. Combing with structure information of Fis-DNA complex, the sp-FRET results are used to estimate the protein induced DNA bending angle distribution and dynamics.

Design of chemical treatment unit for radioactive liquid wastes in Serpong nuclear facilities

International Nuclear Information System (INIS)

Salimin, Z.; Walman, E.; Santoso, P.; Purnomo, S.; Sugito; Suwardiyono; Wintono

1996-01-01

The chemical treatment unit for radioactive liquid wastes arising from nuclear fuel fabrication, radioisotopes production and radiometallurgy facility has been designed. The design of chemical processing unit is based on the characteristics of liquid wastes containing fluors from uranium fluoride conversion process to ammonium uranyl carbonate on the fuel fabrication. The chemical treatment has the following process steps: coagulation-precipitation of fluoride ion by calcium hydroxide coagulant, separation of supernatant solution from sludge, coagulation of remaining fluoride on the supernatant solution by alum, separation of supernatant from sludge, and than precipitation of fluors on the supernatant by polymer resin WWS 116. The processing unit is composed of 3 storage tanks for raw liquid wastes (capacity 1 m 3 per tank), 5 storage tanks for chemicals (capacity 0.5 m 3 per tank), 2 mixing reactors (capacity 0.5 m 3 per reactor), 1 storage tank for supernatant solution (capacity 1 m 3 ), and 1 storage tank for sludge (capacity 1 m 3 )

[In vivo retention of KOH soluble and firmly bound fluoride in demineralized dental enamel].

Science.gov (United States)

Hellwig, E; Klimek, J; Albert, G

1989-03-01

Cylindrical enamel blocks with initial carious lesions were treated for one hour with Duraphat or Fluor-Protector. After removal of the fluoride varnishes the enamel blocks were kept in the mouths of 3 probands for 5 days. Plaque was allowed to accumulate on half of the enamel cylinders, while the other half was kept clean. Part of the enamel cylinders were retained as fluoridated controls. Compared with Duraphat the application of Fluor-Protector resulted in a significantly higher uptake of KOH soluble and firmly bound fluoride. During the 5 days of the experiment the amount of KOH soluble fluorides decreased in both groups. In the presence of plaque the fluoride loss was higher. The amount of firmly bound fluoride increased both in the plaque covered and in the clean enamel. The durable cariostatic effect of fluoridated varnishes seems to be due to the slow dissolution of Ca F2-like precipitates on the enamel surface and the concomitant fluoride uptake in the underlying demineralized enamel.

NIR FRET Fluorophores for Use as an Implantable Glucose Biosensor

Directory of Open Access Journals (Sweden)

Majed DWEIK

2008-12-01

Full Text Available Development of an in vivo optical sensor requires the utilization of Near Infra Red (NIR fluorophores due to their ability to operate within the biological tissue window. Alexa Fluor 750 (AF750 and Alexa Fluor 680 (AF680 were examined as potential NIR fluorophores for an in vivo fluorescence resonance energy transfer (FRET glucose biosensor. AF680 and AF750 found to be a FRET pair and percent energy transfer was calculated. Next, the tested dye pair was utilized in a competitive binding assay in order to detect glucose. Concanavalin A (Con A and dextran have binding affinity, but in the presence of glucose, glucose displaces dextran due to its higher affinity to Con A than dextran. Finally, the percent signal transfer through porcine skin was examined. The results showed with approximately 4.0 mm porcine skin thickness, 1.98 % of the fluorescence was transmitted and captured by the detector.

Glucose Binding Protein as a Novel Optical Glucose Nanobiosensor

Directory of Open Access Journals (Sweden)

Majed DWEIK

2009-11-01

Full Text Available Development of an in vivo optical sensor requires the utilization of Near Infra Red (NIR fluorophores due to their ability to operate within the biological tissue window. Alexa Fluor 750 (AF750 and Alexa Fluor 680 (AF680 were examined as potential NIR fluorophores for an in vivo fluorescence resonance energy transfer (FRET glucose biosensor. AF680 and AF750 found to be a FRET pair and percent energy transfer was calculated. Next, the tested dye pair was utilized in a competitive binding assay in order to detect glucose. Concanavalin A (Con A and dextran have binding affinity, but in the presence of glucose, glucose displaces dextran due to its higher affinity to Con A than dextran. Finally, the percent signal transfer through porcine skin was examined. The results showed with approximately 4.0 mm porcine skin thickness, 1.98 % of the fluorescence was transmitted and captured by the detector.

Nonclassical polarization effects in fluorescence emission spectra from microdroplets

Science.gov (United States)

Arnold, S.; Goddard, N. L.; Hill, S. C.

1999-12-01

We report a pronounced nonclassical polarization effect on the shape of fluorescence emission spectra from isolated microdroplets containing a dilute solution of soluble fluors or a dilute layer of surfactant fluors. We see different spectral shapes for 90° scattering when comparing between IVV, IVH, IHH, IHV. However, we measure the largest difference in spectral shape in the surfactant case, with the incident polarization directed toward the detector (IHV vs IHH). Imaging reveals that the emission in this case principally arises from two distinct regions near the surface of the droplet, which are diametrically opposed and along the axis of the incident laser beam. The effect appears to be the direct result of coupling between molecular emission moments and electromagnetic modes of the droplet. It is not the molecule which radiates but the molecule microvessel. Directional emission is sensitive to the polarization of the electromagnetic mode which is stimulated by the coupling.

SGN-Reseau Eurisys participates to the Hanford military site rehabilitation

International Nuclear Information System (INIS)

Anon.

1996-01-01

Numatec Hanford Corporation, a subsidiary company of SGN-Reseau Eurisys and Cogema, gained with Fluor Daniel the contract for the rehabilitation of the old military nuclear centre of Hanford (Washington, USA). This contract of 5 years represents 5 billions of US dollars with 300 millions of dollars for the French part. This short paper gives a general description of the Hanford installations and of the partners involved in the contract: Fluor Daniel consortium, Lockheed Martin, Babcock and Wilcox, Duke Engineering and Services, Rust Federal Services, Numatec Hanford Corporation (NHC), SGN-Eurisys Services Corporation (SESC). The schedule comprises: the stabilisation of the residual plutonium in all installations before December 1999, the removal of muds and debris from the K storage pool of irradiated fuels before June 2000, the draining and cleaning of the high activity storage tanks before December 2001 and the general decontamination of the installations up to the year 2005. (J.S.)

Fluor determination by alkaline hydrolysis of the uranium and thorium fluorides; Determinacion de fluor por hidrolisis alcalina en fluoruros de uranio y torio

Energy Technology Data Exchange (ETDEWEB)

Barrachina Gomez, L; Gasco Sanchez, L

1961-07-01

The alkaline hydrolysis of the uranium and thorium fluorides is studded and a new method for the determination of the fluoride, on the basis of a indirect volumetric titration with standard soda, is proposed. The compounds that may influence the hydrolysis of the uranium fluoride and that may be occasionally found in it as impurities are also studied. the method can be applied to the uranium fluoride except when there is a great quantity of F{sub 2}UO{sub 2} or UO{sub 3} present in the sample. (Author) 20 refs.

A novel quantitative light‑induced fluorescence device for monitoring ...

African Journals Online (AJOL)

2015-08-06

Aug 6, 2015 ... Background: The FluoreCam system is based on an innovative approach to the ... In total, 11 patients with MIH were diagnosed on a visual MIH scale and ... sensitivity and specificity over traditional techniques .... clinician to record the subject's identification, date of visit, ... versus unaffected enamel.

40 CFR 82.19 - Apportionment of baseline consumption allowances for class II controlled substances.

Science.gov (United States)

2010-07-01

... allowances for class II controlled substances. 82.19 Section 82.19 Protection of Environment ENVIRONMENTAL... Consumption Controls § 82.19 Apportionment of baseline consumption allowances for class II controlled... Ineos Fluor Americas HCFC-22 2,546,305 Kivlan & Company HCFC-22 2,081,018 MDA Manufacturing HCFC-22 2...

Neutron Scattering for Materials Science. Materials Research Society Symposium Proceedings, Volume 166

Science.gov (United States)

1990-01-01

technique - with experimentally produced quartz and calcite textures and the texture of a fluor-apatite sample from a dinosaur bone. Figure 1. Schematic...Lett. 58, 2718 (1987). 6. J. W. Cahn, J. Chem. Phys. 42, 93 (1965). 7. W. Haller, in Solid Phase Biochemistry - Analytical and Chemical Aspects

A SAFER ROUTE TO MDI, An assessment of a phosgene free manufacturing process

NARCIS (Netherlands)

Vetter, Jeroen

2010-01-01

Supervisors: - University of Groningen Prof. dr. F. Picchioni, Drs. W.A. Prins, Prof. ir. M.W.M. Boesten, - Fluor B.V. - Haarlem Mr. G. Smit, Dr. J.C. Göebel. Abstract In this research the technological and economical feasibility of a new technology for the production of methylene diphenyl

Synthetic immunostimulatory glycans interference with host cell apoptosis upon of Toxoplasma gondii infection, in vitro

Directory of Open Access Journals (Sweden)

S.H. Eassa

2017-06-01

Full Text Available Toxoplasmosis is a protozoan infection of humans and animals caused by Toxoplasma gondii, and it’s continuous public health and food safety issue. The tachyzoites (Tg of T. gondii are the most important stage, as they come in direct contact with immune cells such as a macrophage. Tg can modulate and prevent apoptosis of immune cells while promoting survival of the pathogen. Infections caused by Tg can be eradicated if immune cells could stimulate apoptosis and kill pathogens upon exposure. Apoptosis is characterized by the release of mediators, namely Caspases (Cas. New means are required for inducing apoptosis and enhance immunity in the infected host cell to control toxoplasmosis. The present study investigated whether Synthetic Immuno-stimulatory Glycans (SIGs influence Cas and Nitric oxide (NO release and led to Tg damage. Galβ1-3Gal-PAA-fluor (SIG1, Fucα1-4GlcNAcβ-PAA-fluor (SIG2 and GlcNAcβ1-3GalNAcα-PAA-fluor (SIG3 constituted samples studied principally. Murine macrophage had been exposed to the Tg then the SIGs effects on Cas and NO production were determined after 20 hours of pathogen phagocytosis. Here we report that the SIGs had potent in vitro activity against T. gondii; SIG2 was more effective than SIG1 and SIG3, representative by SIG2 treated infected macrophages can induced infected macrophages to release Cas1, 3, and 9. Maximum production of NO by infected macrophages was noticed following the expoxure to all SIGs. Therefore the present study provided the method for the selection of SIGs ligands bearing immunostimulatory factor and apoptotic stimuli properties.

The effects of the combination of chlorhexidine/thymol- and fluoride-containing varnishes on the severity of root caries lesions in frail institutionalised elderly people.

Science.gov (United States)

Brailsford, S R; Fiske, J; Gilbert, S; Clark, D; Beighton, D

2002-01-01

To compare the clinical effects of a fluoride-containing varnish (Fluor-Protector) in combination with a chlorhexidine-containing varnish (Cervitec) on existing root caries lesions in a group of frail elderly subjects. A randomised double blind longitudinal study was utilised. Subjects (n = 102) were randomly allocated to a Test or Placebo group. All leathery and soft root caries lesions in all subjects were coated with Fluor-Protector while the lesions in the Test group were also coated with Cervitec and the lesions in the Placebo group were coated with a Placebo varnish. Treatments were repeated five times in a 12-month period. Clinical parameters associated with root caries, measurements of individual lesions and salivary levels of caries associated bacteria were made at intervals. The clinical severity of the lesions in the Test group did not change significantly during the 12-month study period. In the Placebo group the mean lesion width and lesion height and length of exposed root increased significantly and the lesions were significantly closer to the gingival margin. There were no significant changes in the salivary levels of caries-associated microorganisms after 12 months although, in both groups, there was initially a significant reduction in the salivary levels of mutans streptococci. The combination of Fluor-Protector and Cervitec is a useful, simple, quick and non-invasive method for the control and management of existing root caries lesions. The procedure could be performed by a dental hygienist and may be usefully applied in other high-risk groups including persons with Parkinson's disease, debilitating neuromuscular conditions and dry mouth from whatever cause. Copyright 2002 Elsevier Science Ltd.

Characterization of damaging in apatitic materials irradiated with heavy ions and thermally annealed

International Nuclear Information System (INIS)

Tisserand, R.

2004-12-01

Some minerals belonging to the family of apatite are seen to be potential candidates for use as conditioning matrices or transmutation targets for high level nuclear waste management. Indeed, studies of natural nuclear reactors (Oklo) highlighted the strong ability of these minerals to anneal irradiation damage. In order to determine the global behaviour of these materials, we performed a fundamental study on the evolution of irradiation damage induced by various heavy ions in two apatites: a natural phospho-calcic fluor-apatite from Durango and a synthetic sintered mono-silicated fluor-apatite, called britholite. The damage in these materials was measured by using channelling R.B.S. and X-ray diffraction respectively and by determining an amorphization effective radius Re. The results revealed a similar behaviour for both apatites according to the electronic energy deposit at the entrance of the material. In addition, the effect of an isothermal annealing at 300 C was quantified on a mono-silicated britholite previously irradiated with Kr ions. We highlighted in this case the return of the lattice parameters to their initial values, followed by a partial and slow rebuilding of the crystalline lattice versus the annealing time. Finally, we followed the changes in the morphology of etch pits in the Durango fluor-apatite after acid dissolution as a function of the energy deposit by the ions. We showed that the influence of crystallography leads quickly to opening angles close to 30 degrees. The calculation of etching velocities within the irradiated material highlighted that there is a range of deposit energy where the velocity ratio increases strongly before becoming constant. (author)

Efficacy of reduced doses of bentazone assessed by instruments based on measurement of chlorophyll fluorescence

Directory of Open Access Journals (Sweden)

Vojtěch Kocurek

2011-01-01

Full Text Available Chlorophyll fluorescence is a type of radiation emitted by plants as unused part of energy absorbed from sunlight. Its intensity depends on many stress factors, nutrition, weather conditions etc. Methods based on chlorophyll fluorescence measurement have potential to be perspective for the assessment of herbicide efficacy, phytotoxicity or stress influence. Two fluorometers (FluorCam and handy FluorPen were used for measurements in laboratory experiments in 2007–2009. Herbicide Basagran super with active ingredient bentazone (480 g.l−1 was tested using a parameter called ‚Quantum yield of fluorescence‘ (QY in three different doses: the registered dose 2.0 l.ha−1 (100% and lower doses 1.5 l.ha−1 (75% and 1.0 l.ha−1 (50%. Treated experimental plants of redroot pigweed (Amaranthus retroflexus L. were measured in 0, 1, 2, 3, 6, 9 and 15 days after treatment and the results were compared with subjective assessment (estimation and growth parameters. The obtained results showed that the effect of bentazone measured by both devices was statistically significantly different from untreated control. We can also detect herbicide effect earlier by QY measurement than by other methods. Different doses showed significant differences 15 days after treatment for dose 2.0 l.ha−1 in comparison with both reduced doses. The subjective assessment showed significant differences in all terms of measurement (1–15 days after application except of the day of application. Regeneration of plants for which the youngest leaves were not treated by herbicide was observed for all doses. Difference of QY values between used devices was on average 0.119 (higher for FluorCam but statistically insignificant.

Integrated Environment and Safety and Health Management System (ISMS) Implementation Project Plan

Energy Technology Data Exchange (ETDEWEB)

MITCHELL, R.L.

2000-01-10

The Integrated Environment, Safety and Health Management System (ISMS) Implementation Project Plan serves as the project document to guide the Fluor Hanford, Inc (FHI) and Major Subcontractor (MSC) participants through the steps necessary to complete the integration of environment, safety, and health into management and work practices at all levels.

Integrated Environment and Safety and Health Management System (ISMS) Implementation Project Plan

International Nuclear Information System (INIS)

MITCHELL, R.L.

2000-01-01

The Integrated Environment, Safety and Health Management System (ISMS) Implementation Project Plan serves as the project document to guide the Fluor Hanford, Inc (FHI) and Major Subcontractor (MSC) participants through the steps necessary to complete the integration of environment, safety, and health into management and work practices at all levels

Total cross-section measurements on aluminium, carbon, fluorine and hydrogen for d, d reaction neutrons using the coincidence method (1963); Mesure de la section efficace totale de l'aluminium, du carbone, du fluor et de l'hydrogene pour des neutrons de la reaction d, d par la methode des coincidences (1963)

Energy Technology Data Exchange (ETDEWEB)

Ambrosino, G; Sorriaux, A [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1964-07-01

The experiment described consists in the measurement of the total cross-section of various materials: aluminium, carbon, fluorine and hydrogen, for mono-energetic 2.77 MeV neutrons obtained from the d,d reaction. The measurement is carried out by transmission. The neutrons are detected by means of a plastic scintillator mounted on a 56 AVP photomultiplier, and are isolated from all secondary phenomena (background noise, scattered neutrons) by coincidence with helium 3. which particles are associated to the neutrons from the reaction {sup 2}{sub 1}D ({sup 2}{sub 1}D, n) {sup 3}{sub 2}H The helium 3 particles are detected by a PN junction diode used with inverted polarisation. An absorption exponential has been traced out using measurements made on seven aluminium bars. The accuracy of the total cross-section measurements is about 10{sup -2}. (authors) [French] L'experience exposee dans ce rapport consiste en la mesure des sections efficaces totales de differents materiaux: aluminium, carbone, fluor et hydrogene, pour des neutrons monoenergetiques de 2,77 MeV, obtenus par la reaction d,d. La mesure est faite par transmission. Les neutrons sont detectes par un scintillateur plastique monte sur un photomultiplicateur 56 AVP, et sont separes de tout phenomene secondaire (bruit de fond, neutrons diffuses) par coincidence avec les helium 3, particules associees aux neutrons de la reaction {sup 2}{sub 1}D ({sup 2}{sub 1}D, n) {sup 3}{sub 2}H Les helium 3 sont detectes par une diode a jonction PN utilisee en polarisation inverse. Une exponentielle d'absorption a ete tracee a partir de mesures faites sur sept barreaux d'aluminium. La precision des mesures des sections efficaces totales est de l'ordre de 10{sup -2}. (auteurs)

Five different colours solid-state fluorescence of azastilbenes: a new ...

Indian Academy of Sciences (India)

effect of dipolar intermolecular interactions on their fluorescence properties, the results revealed that the emission spectra of 1−5 in ... lasers,1 fluores- cence sensors,2 fluorescent technology,3 nonlinear optics,4 ..... Royal Golden Jubilee Ph.D. Program (Grant no. ... Derrar S N, Sekkal-Rahal M, Guemra K and Derreumaux P.

Integration plan required by performance agreement SM 7.2.1

International Nuclear Information System (INIS)

Diediker, L.P.

1997-01-01

Fluor Daniel Hanford, Inc. and its major subcontractors are in agreement that environmental monitoring performed under the Project Hanford Management Contract is to be done in accordance with a single, integrated program. The purpose of this Integration Plan for Environmental Monitoring is to document the policies, systems, and processes being put in place to meet one key objective: manage and integrate a technically competent, multi-media ambient environmental monitoring program, in an efficient, cost effective manner. Fluor Daniel Hanford, Inc. and its major subcontractors also commit to conducting business in a manner consistent with the International Standards Organization 14000 Environmental Management System concepts. Because the integration of sitewide groundwater monitoring activities is managed by the Environmental Restoration Contractor, groundwater monitoring it is outside the scope of this document. Therefore, for the purpose of this Integration Plan for Environmental Monitoring, the Integrated Environmental Monitoring Program is defined as applicable to all environmental media except groundwater. This document provides recommendations on future activities to better integrate the overall environmental monitoring program, with emphasis on the near-field program. In addition, included is the Fluor Daniel Hanford, Inc. team review of the environmental monitoring activities on the Hanford Site, with concurrence of Pacific Northwest National Laboratory and Bechtel Hanford, Inc. (The narrative provided later in the Discussion Section describes the review and consideration given to each topic.) This document was developed to meet the requirements of the Project Hanford Management Contract performance agreement (SM7.2) and the tenets of the U.S. Department of Energy's Effluent and Environmental Monitoring Planning Process. This Plan is prepared for the U.S. Department of Energy, Richland Operations Office, Environmental Assurance, Permits, and Policy Division

Phase 1 Final Report for In-Situ Tritium Beta Detector

Energy Technology Data Exchange (ETDEWEB)

Berthold, J.W.; Jeffers, L.A.

1998-04-15

The objectives of this three-phase project were to design, develop, and demonstrate a monitoring system capable of detecting and quantifying tritium in situ in ground and surface waters, and in water from effluent lines prior to discharge into public waterways. The tritium detection system design is based on measurement of the low energy beta radiation from the radioactive decay of tritium using a special form of scintillating optical fiber directly in contact with the water to be measured. The system consists of the immersible sensor module containing the optical fiber, and an electronics package, connected by an umbilical cable. The system can be permanently installed for routine water monitoring in wells or process or effluent lines, or can be moved from one location to another for survey use. The electronics will read out tritium activity directly in units of pico Curies per liter, with straightforward calibration. In Phase 1 of the project, we characterized the sensitivity of fluor-doped plastic optical fiber to tritium beta radiation. In addition, we characterized the performance of photomultiplier tubes needed for the system. In parallel with this work, we defined the functional requirements, target specifications, and system configuration for an in situ tritium beta detector that would use the fluor-doped fibers as primary sensors of tritium concentration in water. The major conclusions from the characterization work are: A polystyrene optical fiber with fluor dopant concentration of 2% gave best performance. This fiber had the highest dopant concentration of any fibers tested. Stability may be a problem. The fibers exposed to a 22-day soak in 120 F water experienced a 10x reduction in sensitivity. It is not known whether this was due to the build up of a deposit (a potentially reversible effect) or an irreversible process such as leaching of the scintillating dye.

Faktor - Faktor yang Berhubungan dengan Derajat Kaparahan Karies di Kecamatan Slawi Kabupaten Tegal Propinsi Jawa Tengah Tahun 1998

Directory of Open Access Journals (Sweden)

Febrina Setiawati

2015-10-01

Full Text Available Residence of Slawi Subdistrict, Tegal District Central Java Province have a frequent tea drinking habit with specific characters of high concentrate tea without sugar or a lot of sugar. It is acknowledged that fluor content in tea can prevent dental caries, while sugar, a sucrose type of carbohydrate in the tea is most cariogenic. The subject of the study, regarding the tea drinking habit, is to obtain information about factors correlated to severity of dental caries in residence of the Slawi Subdistrict, Tegal Distric, Central Java Province. The study type is cross sectional with a sample size of 140. The subjects of the study were residents of Slawi Subdistric with age ranging from 18 to 44 years. An interview was conducted to obtain information of tea drinking characteristics. Samples of tea, water and sugar from each subject examined in a laboratory to obtain the information about fluor and and sugar contents in tea. Saliva examination was also conducted to know its buffer capacity and flow rate. Intra oral clinical examination was conducted to obtain DMF-T and plaque scores. Statistical analysis was applied with multiple linear regression methods using SPSS 7.5 and Stata version 4. The result of the study show that caries prevalence of 76.92% in subjects with tea drinking habit without sugar. The subjects had a 2.85 DMF-T score. Meanwhile caries prevalence was 98.15% for subjects with tea drinking habit with sugar. The subjects had a 6.15 DMF-T score. The mean of fluor concentration in all subjects drinking water was 0.11 mg/l, and 5.26% for sugar content. 68.94% of DMF-T score variation concluded in the multiple linear regression model, is showed by the factors in the model.

Influence of gamma irradiation on electro active properties of copolymers of vinylidene fluoride with tetrafluoroethylene

International Nuclear Information System (INIS)

Magerramov, A.M; Rustamova, D.F.

2010-01-01

Full text : Fluoropolymers are known, as one of the most promising polymers of electro active materials and they are very sensitive to radiation, with the prone to decomposition processes. One way to stabilize the piezoelectric properties of Fluor polymers may be their radiation modification of gamma-irradiation. The aim of the present work is to study the effect of gamma-irradiation on the piezoelectric properties of the copolymer of vinylidene fluoride with tetrafluoroethylene-P (VDF-TeFE). The polarization produced in the optimal range for the electric field. Irradiation of the films performed at the facility Rahm-gamma-30 both before and after polarization. For gamma irradiation, apparently as a result of conformational transitions alpha and beta is a change of relations of ferroelectric and par electric phases in upward of crystallinity, i e an increase in the ferroelectric polymer. It was discussed possible mechanisms of changes in the dielectric, piezoelectric and ferroelectric properties (hysteresis loops, the values of the coercive field) with a residual polarization of Fluor polymers when exposed to gamma-irradiation.

PROTECTING GROUNDWATER & THE COLUMBIA RIVER AT THE HANFORD SITE

Energy Technology Data Exchange (ETDEWEB)

GERBER, M.S.

2006-06-29

Along the remote shores of the Columbia River in southeast Washington state, a race is on. Fluor Hanford, a prime cleanup contractor to the U.S. Department of Energy (DOE) at the Hanford Site, is managing a massive, multi-faceted project to remove contaminants from the groundwater before they can reach the Columbia. Despite the daunting nature and size of the problem--about 80 square miles of aquifer under the site contains long-lived radionuclides and hazardous chemicals--significant progress is being made. Many groups are watching, speaking out, and helping. A large. passionate, diverse, and geographically dispersed community is united in its desire to protect the Columbia River--the eighth largest in the world--and have a voice in Hanford's future. Fluor Hanford and the DOE, along with the US. Environmental Protection Agency (EPA) and the Washington Department of Ecology (Ecology) interact with all the stakeholders to make the best decisions. Together, they have made some remarkable strides in the battle against groundwater contamination under the site.

Fluorescently labeled dengue viruses as probes to identify antigen-specific memory B cells by multiparametric flow cytometry.

Science.gov (United States)

Woda, Marcia; Mathew, Anuja

2015-01-01

Low frequencies of memory B cells in the peripheral blood make it challenging to measure the functional and phenotypic characteristics of this antigen experienced subset of B cells without in vitro culture. To date, reagents are lacking to measure ex vivo frequencies of dengue virus (DENV)-specific memory B cells. We wanted to explore the possibility of using fluorescently labeled DENV as probes to detect antigen-specific memory B cells in the peripheral blood of DENV immune individuals. Alexa Fluor dye-labeled DENV yielded viable virus that could be stored at -80°C for long periods of time. Using a careful gating strategy and methods to decrease non-specific binding, we were able to identify a small frequency of B cells from dengue immune individuals that bound labeled DENV. Sorted DENV(+) B cells from immune, but not naïve donors secreted antibodies that bound DENV after in vitro stimulation. Overall, Alexa Fluor dye-labeled DENVs are useful reagents to enable the detection and characterization of memory B cells in DENV immune individuals. Copyright © 2014 Elsevier B.V. All rights reserved.

Radiation Area Remedial Action FY 1996 summary report

International Nuclear Information System (INIS)

Hayward, W.M.

1996-11-01

The Radiation Area Remedial Action (RARA) project is responsible for the interim stabilization and maintenance of the majority of the inactive waste sites at the Hanford Site. These waste sites include approximately 400 individual sites and 4,000 acres. Three facilities were removed from the RARA list near the end of fiscal year (FY) 1996P: the 218-W-4B, 218-E-10, and 218-E-12B Burial Grounds. These three facilities are treatment, storage, and disposal (TSD) sites assigned to Fluor Daniel Hanford. Therefore, the RARA project will no longer perform activities on the inactive portions to maintain clear lines of responsibility (fully with Fluor Daniel Hanford). RARA activities are broken into two broad categories: interim stabilization, and surveillance and maintenance (S and M). Interim stabilization addresses major corrective actions for sites with radioactive surface contamination. A site continues to require S and M once it has received interim stabilization. Vegetation management is a significant activity under the RARA project. The goal is to control vegetation that may grow and spread radioactive contamination

Azidoperfluoroalkanes: Synthesis and Application in Copper(I)-Catalyzed Azide-Alkyne Cycloaddition

Czech Academy of Sciences Publication Activity Database

Blastik, Zsófia E.; Voltrová, Svatava; Matoušek, V.; Jurásek, Bronislav; Manley, David W.; Klepetářová, Blanka; Beier, Petr

2017-01-01

Roč. 56, č. 1 (2017), s. 346-349 ISSN 1433-7851 EU Projects: European Commission(XE) 607787 - FLUOR21 Institutional support: RVO:61388963 Keywords : azides * click reactions * fluorine * perfluoroalkanes * triazoles Subject RIV: CC - Organic Chemistry OBOR OECD: Organic chemistry Impact factor: 11.994, year: 2016 http://onlinelibrary.wiley.com/doi/10.1002/anie.201609715/full

Canberra Alpha Sentry Installation Functional Design Criteria (FDC)

International Nuclear Information System (INIS)

WHITE, W.F.

1999-01-01

This document provides the functional design criteria for the installation of the Canberra Alpha Sentry System at selected locations within the Plutonium Finishing Plant (PFP). The equipment being installed is identified by part number in Section 3 and the locations are given in Section 5. The design, procurement and installation are assigned to Fluor Federal Services

Water Pollution

International Nuclear Information System (INIS)

Goni, J.

1984-01-01

This work is about the water pollution. The air and the water interaction cycles is the main idea of the geochemical pollution conception. In the water surface as well as in the deep aquifers we can found cough metals or minerals from the athmosferic air. The activities of mercury fluor and nitrates are important to the pollution study

Insurance payment process for HANDI 2000 business management system

Energy Technology Data Exchange (ETDEWEB)

Wilson, D.

1998-08-24

The Pensions and Savings group handles three types of payment into and out of Fluor Daniel Hanford related to insurance benefits: Premium payment to insurance company; Application of employee insurance withholding against insurance costs; Remittance of insurance claims, and administrative fees. General approach in making and recording the remittance is by forwarding payment information to Accounts Payable Master.

Fluor Hanford ALARA Center is a D and D Resource

International Nuclear Information System (INIS)

Waggoner, L.O.

2008-01-01

The mission at the Hanford Nuclear Reservation changed when the last reactor plant was shut down in 1989 and work was started to place all the facilities in a safe condition and begin decontamination, deactivation, decommissioning, and demolition (D and D). These facilities consisted of old shutdown reactor plants, spent fuel pools, processing facilities, and 177 underground tanks containing 53 million gallons of highly radioactive and toxic liquids and sludge. New skills were needed by the workforce to accomplish this mission. By 1995, workers were in the process of getting the facilities in a safe condition and it became obvious improvements were needed in their tools, equipment and work practices. The Hanford ALARA Program looked good on paper, but did little to help contractors that were working in the field. The Radiological Control Director decided that the ALARA program needed to be upgraded and a significant improvement could be made if workers had a place they could visit that had samples of the latest technology and could talk to experienced personnel who have had success doing D and D work. Two senior health physics personnel who had many years experience in doing radiological work were chosen to obtain tools and equipment from vendors and find a location centrally located on the Hanford site. Vendors were asked to loan their latest tools and equipment for display. Most vendors responded and the Hanford ALARA Center of Technology opened on October 1, 1996. Today, the ALARA Center includes a classroom for conducting training and a mockup area with gloveboxes. Two large rooms have a containment tent, several glove bags, samples of fixatives/expandable foam, coating displays, protective clothing, heat stress technology, cutting tools, HEPA filtered vacuums, ventilation units, pumps, hydraulic wrenches, communications equipment, shears, nibblers, shrouded tooling, and several examples of innovative tools developed by the Hanford facilities. See Figures I and II. The ALARA Center staff routinely researches and tests new technology, sponsor vendor demonstrations, and redistribute tools, equipment and temporary shielding that may not be needed at one facility to another facility that needs it. The ALARA Center staff learns about new technology in several ways. This includes past radiological work experience, interaction with vendors, lessons learned, networking with other DOE sites, visits to the Hanford Technical Library, attendance at off-site conferences and ALARA Workshops. Personnel that contact the ALARA Center for assistance report positive results when they implement the tools, equipment and work practices recommended by the ALARA Center staff. This has translated to reduced exposure for workers and reduced the risk of contamination spread. For example: using a hydraulic shear on one job saved 16 Rem of exposure that would have been received if workers had used saws-all tools to cut piping in twenty-nine locations. Currently, the ALARA Center staff is emphasizing D and D techniques on size-reducing materials, decontamination techniques, use of remote tools/video equipment, capture ventilation, fixatives, using containments and how to find lessons learned. The ALARA Center staff issues a weekly report that discusses their interaction with the workforce and any new work practices, tools and equipment being used by the Hanford contractors. Distribution of this weekly report is to about 130 personnel on site and 90 personnel off site. This effectively spreads the word about ALARA throughout the DOE Complex. DOE EM-23, in conjunction with the D and D and Environmental Restoration work group of the Energy Facility Contractors Organization (EFCOG) established the Hanford ALARA Center as the D and D Hotline for companies who have questions about how D and D work is accomplished. The ALARA Center has become a resource to the nuclear industry and routinely helps contractors at other DOE Sites, power reactors, DOD sites, and sites in England, Europe and Indonesia. Other ALARA Centers are located at the Savannah River Site and Los Alamos National Lab

Biotransferencia de fluor a distintos tejidos de pejerreyes (Odontesthes bonariensis)

OpenAIRE

Volpedo, Alejandra; Puntoriero, María Laura; Fernandez Cirelli, Alicia

2016-01-01

The presence of fluorine (F), toxic trace element, has relevance in the trophic chain because it can biotrasfer to different levels. It can come from water as food, being chronic ingestion harmful to human beings. Fluorine was found in different water bodies of southwest of Buenos Aires. Chasicó Lake has the highest fluorine concentration. This water body is the most important for commercial and recreational fishing silverside (Odontesthes bonariensis) of the area. The aim of this paper is to...

Anticaries potential of commercial dentifrices as determined by fluoridation and remineralization efficiency.

Science.gov (United States)

Casals, Elias; Boukpessi, Tchilalo; McQueen, Christina M; Eversole, Sandy L; Faller, Robert V

2007-11-01

The aim of this in vitro study was to investigate fluoride uptake in human enamel after use of commercially available toothpastes containing different fluoride compounds, or combinations of fluoride actives formulated into a single product, as a means of determining the efficiency of each formula for delivering caries preventing fluoride to demineralized (caries active) enamel. Four test dentifrices and two controls were assessed and placed in groups as follows: Group 1: Lacer (Spain); Group 2: Positive control-USP Reference Standard 1100 ppm F; Group 3: Fluocaril Bi-Fluoré 250 (France); Group 4: Colgate Fluor Active (Denmark); Group 5: Elmex (France); and Group 6: A placebo (formulated the same as the USP Reference Standard toothpaste with the exception that it contained < 1 ppm F). Cores 3 mm in diameter were removed from erupted human enamel specimens (extracted by local oral surgeons for orthodontic reasons) and stored in 1% Thymol solution prior to use. They were ground and polished to remove the natural fluoride rich enamel layer, then exposed to a demineralization solution, and assessed for surface microhardness to enable randomization for use in the study. Each group of five specimens underwent a daily pH cycling procedure that involved exposure to pooled human saliva (refreshed three times daily). The groups were then exposed to dentifrice slurries four times daily for one minute per exposure and to a demineralization solution for three hours. The cycling procedure was repeated for five days. Specimens were again analyzed for surface microhardness and fluoride uptake upon completion of five days of treatment. Average surface hardness: Groups 2 and 3 showed a statistically significant greater (p<0.05) change indicating greater remineralization compared to all other groups. The average change was 23.45 for Group 2 and 22.65 for Group 3. All other groups had changes ranging from 4.25-8.62. No other statistically significant differences were observed between

X-ray streak and framing camera techniques

International Nuclear Information System (INIS)

Coleman, L.W.; Attwood, D.T.

1975-01-01

This paper reviews recent developments and applications of ultrafast diagnostic techniques for x-ray measurements. These techniques, based on applications of image converter devices, are already capable of significantly important resolution capabilities. Techniques capable of time resolution in the sub-nanosecond regime are being considered. Mechanical cameras are excluded from considerations as are devices using phosphors or fluors as x-ray converters

Results of dilution studies with waste from tank 241-AN-104

International Nuclear Information System (INIS)

HERTING, D.L.

1999-01-01

This report documents the completion of the B and W Hanford Company functional and Safety Review Board reviews and the Fluor Daniel Hanford review for the Plutonium Finishing Plant (PFP) Final Safety Analysis Report (FSAR), HNF-SD-CP-SAR-021, Revision 1. The reviews for the FSAR were conducted during the period from December 9, 1998 to January 14, 1999

Radiation damage studies on polystyrene-based scintillators

International Nuclear Information System (INIS)

Britvich, G.I.; Peresypkin, A.I.; Rykalin, V.I.

1991-01-01

The radiation resistance of polystyrene-based scintillators containing various scintillation dopes is reported. All samples were irradiated to 137 Cs gamma rays in air at room temperature. The examination of radiation resistance of about thirty fluorescence compounds has been made. The most radiation-hard fluores are X25, X31, 3HF and M3HF. 1 fig.; 6 tabs

Study of copper fluorination; Contribution a l'etude de la fluoruration du cuivre

Energy Technology Data Exchange (ETDEWEB)

Gillardeau, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1967-02-01

This report deals with the action of fluorine on copper. Comprehensive descriptions are given of the particular technological methods and of the preparation of the reactants. This fluorination reaction has been studied at medium and low fluorine pressures. A nucleation and growth phenomenon is described. The influence of a pollution of the gas phase on the fluorination process is described. The solid-state reaction between cupric fluoride and cooper has also been studied. A special study has been made of the growth of copper deposits by thermal decomposition of gaseous fluorides. (author) [French] Ce rapport traite de la reaction du fluor sur le cuivre. Les methodes technologiques particulieres ainsi que les preparations des reactifs sont largement developpees. Cette reaction de fluoruration est etudiee sous pressions moyennes et sous basses pressions de fluor. Un phenomene de germination et croissance est decrit. L'influence de la pollution de la phase gazeuse sur le processus de fluoruration est rapportee. La reaction a l'etat solide du fluorure cuivrique sur le cuivre a egalement ete etudiee. La croissance de depots de cuivre par decomposition thermique des fluorures de cuivre gazeux fait l'objet d'une etude speciale. (auteur)

Nanoscale control of Ag nanostructures for plasmonic fluorescence enhancement of near-infrared dyes

KAUST Repository

Xie, Fang

2013-05-23

Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based detection techniques. Metal induced fluorescence enhancement offers the possibility of increasing the sensitivity of protein detection in clinical applications. We report the use of tunable plasmonic silver nanostructures for the fluorescence enhancement of a near-infrared (NIR) dye (Alexa Fluor 790). Extensive fluorescence enhancement of ∼2 orders of magnitude is obtained by the nanoscale control of the Ag nanostructure dimensions and interparticle distance. These Ag nanostructures also enhanced fluorescence from a dye with very high quantum yield (7.8 fold for Alexa Fluor 488, quantum efficiency (Qy) = 0.92). A combination of greatly enhanced excitation and an increased radiative decay rate, leading to an associated enhancement of the quantum efficiency leads to the large enhancement. These results show the potential of Ag nanostructures as metal induced fluorescence enhancement (MIFE) substrates for dyes in the NIR "biological window" as well as the visible region. Ag nanostructured arrays fabricated by colloidal lithography thus show great potential for NIR dye-based biosensing applications. [Figure not available: see fulltext.] © 2013 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.

A fusion-spliced near-field optical fiber probe using photonic crystal fiber for nanoscale thermometry based on fluorescence-lifetime measurement of quantum dots.

Science.gov (United States)

Fujii, Takuro; Taguchi, Yoshihiro; Saiki, Toshiharu; Nagasaka, Yuji

2011-01-01

We have developed a novel nanoscale temperature-measurement method using fluorescence in the near-field called fluorescence near-field optics thermal nanoscopy (Fluor-NOTN). Fluor-NOTN enables the temperature distributions of nanoscale materials to be measured in vivo/in situ. The proposed method measures temperature by detecting the temperature dependent fluorescence lifetimes of Cd/Se quantum dots (QDs). For a high-sensitivity temperature measurement, the auto-fluorescence generated from a fiber probe should be reduced. In order to decrease the noise, we have fabricated a novel near-field optical-fiber probe by fusion-splicing a photonic crystal fiber (PCF) and a conventional single-mode fiber (SMF). The validity of the novel fiber probe was assessed experimentally by evaluating the auto-fluorescence spectra of the PCF. Due to the decrease of auto-fluorescence, a six- to ten-fold increase of S/N in the near-field fluorescence lifetime detection was achieved with the newly fabricated fusion-spliced near-field optical fiber probe. Additionally, the near-field fluorescence lifetime of the quantum dots was successfully measured by the fabricated fusion-spliced near-field optical fiber probe at room temperature, and was estimated to be 10.0 ns.

Activatable Optical Imaging with a Silica-Rhodamine Based Near Infrared (SiR700) Fluorophore: A comparison with cyanine based dyes

Science.gov (United States)

McCann, Thomas E.; Kosaka, Nobuyuki; Koide, Yuichiro; Mitsunaga, Makoto; Choyke, Peter L.; Nagano, Tetsuo; Urano, Yasuteru; Kobayashi, Hisataka

2011-01-01

Optical imaging is emerging as an important tool to visualize tumors. However, there are many potential choices among the available fluorophores. Optical imaging probes that emit in the visible range can image superficial tumors with high quantum yields, however, if deeper imaging is needed then near infrared (NIR) fluorophores are necessary. Most commercially available NIR fluorophores are cyanine based and are prone to non-specific binding and relatively limited photostability. Silica-containing rhodamine (SiR) fluorophores represent a new class of NIR fluorophores, which permit photoactivation via H-dimer formation as well as demonstrate improved photostability. This permits higher tumor-to-background ratios (TBRs) to be achieved over longer periods of time. Here, we compared an avidin conjugated with SiR700 (Av-SiR700) to similar compounds based on cyanine dyes (Av-Cy5.5 and Av-Alexa Fluor 680) in a mouse tumor model of ovarian cancer metastasis. We found that the Av-SiR700 probe demonstrated superior quenching enabling activation after binding-internalization to the target cell. As a result, Av-SiR700 had higher TBRs compared to Av-Cy5.5, and better biostability compared to Av-Alexa Fluor 680. PMID:22034863

Scintillators for fiber optics: system sensitivity and bandwidth as a function of fiber length

International Nuclear Information System (INIS)

Lutz, S.S.; Franks, L.A.; Fluornoy, J.M.; Lyons, P.B.

1981-01-01

Scintillators have been employed for several years as ionizing radiation-to-light converters in plasma diagnostic experiments that utilize fiber optics. Until recently, nanosecond and subnanosecond scintillators were available only in the near ultraviolet. However, the bandwidth and transmission properties of fiber optics both strongly favor operation at longer wavelengths. More recently, nanosecond and subnanosecond scintillators with emission peaks around 480 nm have been reported. A time-resolved plasma-imaging experiment using one of these scintillators and 100 channels of graded-index fiber, each 500 m long, has been successfully tested on a nuclear event at the Nevada Test Site. During the past year we have developed several new scintillator systems with emission wavelengths more compatible with fiber optics and with response times in the nanosecond and subnanosecond time region. One scintillator, based on Kodak dye 14567 (DCM), has an emission maximum at 650 nm and a response time (FWHM) of 1.2 ns. Experimental data on system sensitivity and bandwidth versus fiber length are presented for three fluor-fiber systems. Data on fluor formulation, response time, and linearity-of-response are given, and a model for scintillator nonlinearity, based on solvent, radiation-induced, transient absorption, is presented

Human Resources Staffing Plan for the Tank Farm Contractor

International Nuclear Information System (INIS)

BOSLEY, J.W.

2000-01-01

The Human Resources Staffing Plan quantified the equivalent staffing needs required for the Tank Farm Contractor (TFC) and its subcontractors to execute the readiness to proceed baseline between FY 2000-2008. The TFC staffing needs were assessed along with the staffings needs of Fluor Hanford and the privatization contractor. The plan then addressed the staffing needs and recruitment strategies required to execute the baseline

Dementia with impaired glucose metabolism in late onset metachromatic leukodystrophy

DEFF Research Database (Denmark)

Johannsen, P.; Ehlers, L.; Hansen, Hans Jacob

2001-01-01

and attention deficits with a slow psychomotor speed. MR brain imaging displayed confluent hyperintensities of periventricular and subcortical white matter. Low levels of arylsulfatase A confirmed the diagnosis. Impaired cortical glucose metabolism especially of the medial temporal and frontal cortices...... was observed using positron emission tomography and fluor-18-labeled fluorodesoxyglucose. The neuropsychological deficits are related to the location of deficits in glucose metabolism....

Synthesis and nucleophilic aromatic substitution of 3-fluoro-5-nitro-1-(pentafluorosulfanyl)benzene

Czech Academy of Sciences Publication Activity Database

Ajenjo, Javier; Greenhall, M.; Zarantonello, C.; Beier, Petr

2016-01-01

Roč. 12, Feb 3 (2016), s. 192-197 ISSN 1860-5397 EU Projects: European Commission(XE) 607787 - FLUOR21 Institutional support: RVO:61388963 Keywords : direct fluorination * fluorine * nucleophilic aromatic substitution * pentafluorosulfanyl group * vicarious nucleophilic substitution Subject RIV: CC - Organic Chemistry Impact factor: 2.337, year: 2016 http://www.beilstein-journals.org/bjoc/single/articleFullText.htm?publicId=1860-5397-12-21

Role of Integrin Alpha 5 in ErbB2-Mediated Breast Cancer

Science.gov (United States)

2009-09-01

AD__ ( Leave blank) _______________ Award Number: W81XWH-08-1...3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 using Guava Technologies EasyCyte Plus, and data analyzed with Guava ...dilution, washed, and incubated with secondary antibody (AlexaFluor mouse 488, Invitrogen) at a 1:200 dilution. Cells were read on a Guava Technologies

Positron emission tomography. Basic principles

International Nuclear Information System (INIS)

Rodriguez, Jose Luis; Massardo, Teresa; Gonzalez, Patricio

2001-01-01

The basic principles of positron emission tomography (PET) technique are reviewed. lt allows to obtain functional images from gamma rays produced by annihilation of a positron, a positive beta particle. This paper analyzes positron emitters production in a cyclotron, its general mechanisms, and the various detection systems. The most important clinical applications are also mentioned, related to oncological uses of fluor-l8-deoxyglucose

High effective atomic number polymer scintillators for gamma ray spectroscopy

Science.gov (United States)

Cherepy, Nerine Jane; Sanner, Robert Dean; Payne, Stephen Anthony; Rupert, Benjamin Lee; Sturm, Benjamin Walter

2014-04-15

A scintillator material according to one embodiment includes a bismuth-loaded aromatic polymer having an energy resolution at 662 keV of less than about 10%. A scintillator material according to another embodiment includes a bismuth-loaded aromatic polymer having a fluor incorporated therewith and an energy resolution at 662 keV of less than about 10%. Additional systems and methods are also presented.

UPDATE HANFORD SITE D and D PROGRAMS ACCELERATE EXPAND

International Nuclear Information System (INIS)

GERBER, M.S.

2004-01-01

A large, new decontamination and decommissioning organization targeted toward rapid, focused work on aging and highly contaminated structures was formed at the DOE's Hanford Site in southeast Washington state in autumn 2003. Managed by prime contractor Fluor Hanford, the new organization has made significant progress during its first six months. Under the direction of Mike Lackey, who recently joined Fluor from the Portland General Electric Trojan Plant, the Fluor Hanford DandD organization is tackling the Plutonium Finishing Plant (PFP) complex and the Fast Flux Test Facility (FFTF), and is nearly finished demolishing the 233-S Plutonium Concentration Facility. In addition, the DandD organization is progressing through the development and public comment phases of its required environmental permitting, planning work and procurement services to DandD three other Hanford facilities: 224-T and 224-B Plutonium Concentration Facilities, and the U Plant radiochemical processing facility. It is also planning and beginning to DandD the spent fuel handling areas of the Site's 100-K Reactor Area. The 586-square mile Hanford Site, the oldest plutonium production center in the world, served as the ''workhorse'' of the American nuclear defense arsenal from 1944 through 1989. Hanford produced the special nuclear material for the plutonium cores of the Trinity (test) and Nagasaki explosions, and then went on to produce more than half of the weapons plutonium ever manufactured by the United States, and about one-fourth of that manufactured worldwide. As a result, Hanford, the top-secret ''Paul Bunyan'' in the desert, is one of the most contaminated areas in the world. Its cleanup agreement with state and federal regulators, known as the ''Tri-Party Agreement,'' celebrates its 15th anniversary this spring, at a time when operations dealing with unstable plutonium leftovers, corroded spent fuel, and liquids wastes in single-shelled tanks conclude. As these crucial jobs are coming to

Human endothelial progenitor cells internalize high-density lipoprotein.

Directory of Open Access Journals (Sweden)

Kaemisa Srisen

Full Text Available Endothelial progenitor cells (EPCs originate either directly from hematopoietic stem cells or from a subpopulation of monocytes. Controversial views about intracellular lipid traffic prompted us to analyze the uptake of human high density lipoprotein (HDL, and HDL-cholesterol in human monocytic EPCs. Fluorescence and electron microscopy were used to investigate distribution and intracellular trafficking of HDL and its associated cholesterol using fluorescent surrogates (bodipy-cholesterol and bodipy-cholesteryl oleate, cytochemical labels and fluorochromes including horseradish peroxidase and Alexa Fluor® 568. Uptake and intracellular transport of HDL were demonstrated after internalization periods from 0.5 to 4 hours. In case of HDL-Alexa Fluor® 568, bodipy-cholesterol and bodipy-cholesteryl oleate, a photooxidation method was carried out. HDL-specific reaction products were present in invaginations of the plasma membrane at each time of treatment within endocytic vesicles, in multivesicular bodies and at longer periods of uptake, also in lysosomes. Some HDL-positive endosomes were arranged in form of "strings of pearl"- like structures. HDL-positive multivesicular bodies exhibited intensive staining of limiting and vesicular membranes. Multivesicular bodies of HDL-Alexa Fluor® 568-treated EPCs showed multilamellar intra-vacuolar membranes. At all periods of treatment, labeled endocytic vesicles and organelles were apparent close to the cell surface and in perinuclear areas around the Golgi apparatus. No HDL-related particles could be demonstrated close to its cisterns. Electron tomographic reconstructions showed an accumulation of HDL-containing endosomes close to the trans-Golgi-network. HDL-derived bodipy-cholesterol was localized in endosomal vesicles, multivesicular bodies, lysosomes and in many of the stacked Golgi cisternae and the trans-Golgi-network Internalized HDL-derived bodipy-cholesteryl oleate was channeled into the lysosomal

A quantum dot-immunofluorescent labeling method to investigate the interactions between a crinivirus and its whitefly vector

Directory of Open Access Journals (Sweden)

James C. K. Ng

2013-04-01

Full Text Available Successful vector-mediated plant virus transmission entails an intricate but poorly understood interplay of interactions among virus, vector, and plant. The complexity of interactions requires continually improving/evaluating tools and methods for investigating the determinants that are central to mediating virus transmission. A recent study using an organic fluorophore (Alexa Fluor-based immunofluorescent localization assay demonstrated that specific retention of Lettuce infectious yellows virus (LIYV virions in the anterior foregut or cibarium of its whitefly vector is required for virus transmission. Continuous exposure of organic fluorophore to high excitation light intensity can result in diminished or loss of signals, potentially confounding the identification of important interactions associated with virus transmission. This limitation can be circumvented by incorporation of photostable fluorescent nanocrystals, such as quantum dots (QDs, into the assay. We have developed and evaluated a QD-immunofluorescent labeling method for the in vitro and in situ localization of LIYV virions based on the recognition specificity of streptavidin-conjugated QD605 (S-QD605 for biotin-conjugated anti-LIYV IgG (B-αIgG. IgG biotinylation was verified in a blot overlay assay by probing SDS-PAGE separated B-αIgG with S-QD605. Immunoblot analyses of LIYV using B-αIgG and S-QD605 resulted in a virus detection limit comparable to that of DAS-ELISA. In membrane feeding experiments, QD signals were observed in the anterior foregut or cibarium of virion-fed whitefly vectors but absent in those of virion-fed whitefly non-vectors. Specific virion retention in whitefly vectors corresponded with successful virus transmission. A fluorescence photobleaching assay of viruliferous whiteflies fed B-αIgG and S-QD605 vs. those fed anti-LIYV IgG and Alexa Fluor 488-conjugated IgG revealed that QD signal was stable and deteriorated ∼7 to 8 fold slower than that of Alexa

The Reactions of Hot Fluorine-18 with Gaseous Carbon Tetrafluoride; Reactions des Atomes {sup 18}F Chauds avec le Tetrafluorure de Carbone en Phase Gazeuse; Reaktsii goryachikh atomov ftora-18 s gazovoj fazoj tetraftormetana; Reacciones de Atomos Calientes de Fluor-18 con Tetrafluoruro de Carbono Gaseoso

Energy Technology Data Exchange (ETDEWEB)

Colebourne, N.; Todd, J. F.J.; Wolfgang, R. [Yale University, New Haven, CT (United States)

1965-04-15

'electrons de l'Universite Yale. Le processus {sup 19}F ({gamma}, n){sup 18}F donne des atomes de {sup 18}F d'une energie cinetique de l'ordre de 10{sup 5} a 10{sup 6} eV. Ces especes perdent de l'energie par choc et doivent atteindre la gamme des energies 'chimiques' (< 100 eV) en tant qu'atomes a l'etat fondamental. Les auteurs ont constate que Vethylene etait un excellent agent de balayage pour les atomes {sup 18}F thermiques. Ils ont analyse les produits a l'aide de methodes courantes de radiochromatographie en phase gazeuse. Le systeme s'est revele tres sensible aux effets de rayonnements etrangers; aussi les mesures de precaution appropriees ont-elles ete prises. Des reactions de deplacement, analbgues a celles qui ont ete observees pour l'hydrogene chaud mais beaucoup moins efficaces, ont ete relevees; ce sont les reactions suivantes: {sup 18}F + {sup 4}CF --> CF{sub 3}{sup 18}F + F, {sup 18}F + CF{sub 4} --> CF{sub 2}{sup 18}F + (F + F), Il n'a pas ete possible d'etudier directement la reaction d'enlevement {sup 18}F + CF{sub 4} --> CF{sub 3} + F{sup 18}F. Cependant, certains indices donnent a penser que cette reaction a egalement une faible efficacite. Les auteurs ont etudie en detail l'effet de ralentissement sur le systeme {sup 18}F + CF{sub 4}. Ils ont analyse les donnees obtenues en se fondant sur la theorie cinetique des reactions chaudes. Ils ont constate que le systeme etait conforme a cette theorie, ce qui a fourni une confirmation quantitative de l'interpretation actuelle des resultats. Les systemes methane et tetrafluorure de carbone fournissent une base a certaines conclusions provisoires sur les mecanismes des reactions des atomes de fluor chauds. Il semble a present que, moyennant certaines modifications importantes mais naturelles, le modele qui avait tout d'abord ete mis au point pour les atomes d'hydrogene chauds soit applicable aux atomes de fluor chauds. (author) [Spanish] La memoria describe estudios de las reacciones de atomos calientes de {sup 18}F

Air and smear sample calculational tool for Fluor Hanford Radiological control

International Nuclear Information System (INIS)

BAUMANN, B.L.

2003-01-01

A spreadsheet calculation tool was developed to automate the calculations performed for determining the concentration of airborne radioactivity and smear counting as outlined in HNF--13536, Section 5.2.7, ''Analyzing Air and Smear Samples''. This document reports on the design and testing of the calculation tool. Radiological Control Technicians (RCTs) will save time and reduce hand written and calculation errors by using an electronic form for documenting and calculating work place air samples. Current expectations are RCTs will perform an air sample and collect the filter or perform a smear for surface contamination. RCTs will then survey the filter for gross alpha and beta/gamma radioactivity and with the gross counts utilize either hand calculation method or a calculator to determine activity on the filter. The electronic form will allow the RCT with a few key strokes to document the individual's name, payroll, gross counts, instrument identifiers; produce an error free record. This productivity gain is realized by the enhanced ability to perform mathematical calculations electronically (reducing errors) and at the same time, documenting the air sample

Fluor determination by alkaline hydrolysis of the uranium and thorium fluorides

International Nuclear Information System (INIS)

Barrachina Gomez, L.; Gasco Sanchez, L.

1961-01-01

The alkaline hydrolysis of the uranium and thorium fluorides is studded and a new method for the determination of the fluoride, on the basis of a indirect volumetric titration with standard soda, is proposed. The compounds that may influence the hydrolysis of the uranium fluoride and that may be occasionally found in it as impurities are also studied. the method can be applied to the uranium fluoride except when there is a great quantity of F 2 UO 2 or UO 3 present in the sample. (Author) 20 refs

Characterization of an anthraquinone fluor from the bioluminescent, pelagic polychaete Tomopteris

Science.gov (United States)

Francis, Warren R; Powers, Meghan L; Haddock, Steven H D

2014-01-01

Tomopteris is a cosmopolitan genus of polychaetes. Many species produce yellow luminescence in the parapodia when stimulated. Yellow bioluminescence is rare in the ocean, and the components of this luminescent reaction have not been identified. Only a brief description, half a century ago, noted fluorescence in the parapodia with a remarkably similar spectrum to the bioluminescence, which suggested that it may be the luciferin or terminal light-emitter. Here, we report the isolation of the fluorescent yellow–orange pigment found in the luminous exudate and in the body of the animals. Liquid chromatography-mass spectrometry revealed the mass to be 270 m/z with a molecular formula of C15H10O5, which ultimately was shown to be aloe-emodin, an anthraquinone previously found in plants. We speculate that aloe-emodin could be a factor for resonant-energy transfer or the oxyluciferin for Tomopteris bioluminescence. PMID:24760626

Re-injection accelerates groundwater clean up at Fernald, Fluor Fernald, Inc

International Nuclear Information System (INIS)

Dave Brettschneider; William Hertel; Ken Broberg

2000-01-01

A successful one year long, field scale demonstration of the use of groundwater re-infection at Fernald was recently completed bringing DOE one step closer to achieving an accelerated site remediation (DOE 2000). The demonstration marks the end of a several year effort to evaluate whether: re-injection could be conducted efficiently at Fernald, and if the approved aquifer remedy at Fernald would benefit by incorporating re-infection. Evaluation of re-injection technology involved not only technical considerations, but also participation and cooperation of regulators and stakeholders. The demonstration was considered to be unique in that it was integrated into the design of the current approved aquifer remedy and utilized the existing remediation infrastructure. Information collected during the demonstration indicated that re-injection wells could be operated efficiently at Fernald and that the current approved groundwater remedy should be modified to include the use of re-injection

European Scientific Notes. Volume 37, Numbers 12.

Science.gov (United States)

1983-12-01

being flown on GAMMA-i, a Russian the local matter is not in the form of mission planned for 1984-85 as a French- ordinary baryonic mass (e.g., neutrons...probe the structure of the electric field in collective ion acceleration experiments. Energy-Transfer Processes in Condensed Matter ...Denmark) examined distribu- fields in straits regions. tions of suspended matter and fluore- Other areas of straits research are scence in straits

Integrative Lifecourse and Genetic Analysis of Military Working Dogs

Science.gov (United States)

2013-10-01

FITC) and Alexa Fluor 647 (BD Biosciences), respectively. Secondary antibodies of either phycoerythrin (PE; 5 μL) or fluorescein isothiocyanate (FITC; 1...zone B cells [2]. Cytokines/cytokine receptors, Ig recognition , and antigen presented by APCs, dendritic cells, and/or macrophages can initiate...MetaMap is targeted at finding medical terms from a biomedical text, it leverages a combination of part-of- speech tagging, shallow parsing, and longest

First Cases of Spotted Fever Group Rickettsiosis in Thailand

Science.gov (United States)

1994-01-01

discernible fluorescing organisms. MATERIALS AND METHODS Fluore-cein-conjugated rabbit anti-human iam- All three patients were seen at Chiang Mai munoglobulin...IgG) (heavy and light chain spe- University Hospital. Chiang Mai (population cific: Cappel Laboratories, Cochranville. PA) 1.300,000) is one of the 76...ulty of Medicine at Chiang Mai University. established procedures.." Horseradish peroxi- Chiang Mai University Hospital. a 1.000-bed dase-labeled mouse

Transplantation and Perfusion of Microvascular Fragments in a Rodent Model of Volumetric Muscle Loss Injury

Science.gov (United States)

2014-07-01

and eosin (H&E) or Wheat Germ Agglutinin (WGA), Alexa Fluor® 488 conjugate (1:50 dilution, Invitrogen, Carlsbad, CA, USA) to stain for cellular...1:500), Invitrogen). Tissue containing MVFs isolated from GFP transgenic animals were sectioned as described above, fixed with 4... transgenic animals were transplanted into VML defects to determine if the vascularity we observed was a result of the transplanted vessels or was due to

Minutes of the Explosives Safety Seminar (25th) Held in Anaheim, California on 18-20 August 1992. Volume 3

Science.gov (United States)

1992-08-20

Secretary of Defense for Environment KEYNOTE ADDRESS ...................................................... 7 Mr. Colin McMillan, Assistant Secretary...11-547 McGuinness, Jack 111-161 Soleau, Edward W. 11-123 McMahon, Gordon W. 11-277 Song, So-Young 1-429, 11-85 McMillan, Colin 1-7 Spivey, Kathy H. 11...DRAKE JAMES APPLIED RESEARCH ASSOC. INC. MR. DRAKE RICHARD FLUOR DANIEL MR. DRURY CHUCK SHAMROCK SERVICES MR. DUA BALBIR DAY & ZIMMERMANN, INC. MR

Transparent lithiated polymer films for thermal neutron detection

Energy Technology Data Exchange (ETDEWEB)

Mabe, Andrew N., E-mail: andrew.n.mabe@gmail.com [Department of Chemistry, University of Tennessee, Knoxville, TN 37996 (United States); Auxier, John D. [Department of Chemistry, University of Tennessee, Knoxville, TN 37996 (United States); Urffer, Matthew J. [Department of Nuclear Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Penumadu, Dayakar [Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Schweitzer, George K. [Department of Chemistry, University of Tennessee, Knoxville, TN 37996 (United States); Miller, Laurence F. [Department of Nuclear Engineering, University of Tennessee, Knoxville, TN 37996 (United States)

2013-09-11

Novel water-soluble {sup 6}Li loaded copolymer scintillation films have been designed and fabricated to detect thermal neutrons. Styrene and maleic anhydride were copolymerized to form an alternating copolymer, then the anhydride functionality was hydrolyzed using {sup 6}Li hydroxide. The resulting poly(styrene-co-lithium maleate) was mixed with salicylic acid as a fluor and cast as a thin film from water. The maximum {sup 6}Li loading obtained that resulted in a transparent film was 4.36% by mass ({sup 6}Li to polymer). The optimum fluorescence output was obtained for 11.7% salicylic acid by mass, presumably in the form of lithium salicylate, resulting in an optimum film containing 3.85% by mass of {sup 6}Li. A facile and robust synthesis method, film fabrication protocol, photoluminescence results, and scintillation responses are reported herein. -- Highlights: • A transparent polymer scintillator containing 3.85 wt% {sup 6}Li has been synthesized. • This class of polymeric thermal neutron scintillation detector is water-soluble. • Salicylic acid, presumably in the form of lithium salicylate, is used as a fluor. • The material emits 373 photons/α ({sup 241}Am) and an average of 139 photons/β ({sup 36}Cl). • The material emits 360 photons per thermal neutron capture event.

The influence of varnish and high fluoride on erosion and abrasion in a laboratory investigation.

Science.gov (United States)

Sar Sancakli, H; Austin, R S; Al-Saqabi, F; Moazzez, R; Bartlett, D

2015-03-01

The aim of this study was to investigate the potential of concentrated fluoride varnishes to reduce enamel loss from repeated cycles of citric acid erosion and toothbrush abrasion in vitro. Polished human enamel samples were exposed to fluoride varnishes: Bifluorid10® (NaF&CaF2- 45,200 ppmF), Duraphat® (NaF 22,600 ppmF), Fluor-Protector® (difluorsilane 1000 ppmF) and a control coating of copal ether varnish (0 ppmF) group and a deionized water group. For each group of 16 samples, nine cycles of erosion and nine cycles of erosion-abrasion [1 cycle=erosion (0.3% citric acid, pH 3.2, 5 min)+artificial saliva (1 h, pH 7.0)+abrasion (120 linear strokes in artificial saliva from Oral B medium soft brushes 300 g loading]. The change in the enamel surface was evaluated using optical profilometry. Duraphat® and Bifluorid10® applications had a median (IQR) step height of 4.21 um (1.59) and 5.01 um (1.02). This was statistically significantly less than Fluor-Protector® 6.83 um (1.25), copal ether 7.22 um (1.97) and water 7.39 um (1.96) (pDental Association.

Reengineering and health physics within the project Hanford management contract

International Nuclear Information System (INIS)

Atencio, E.M.

1997-01-01

The impending transition of the Hartford Site management and operations (M ampersand O) contract to a management and integrating (M ampersand I) contract format, together with weak radiological performance assessments by external organizations and reduced financial budgets prompted the 're-engineering' of the previous Hanford prime contractor Radiological Control (Rad Con) organization. This paper presents the methodology, identified areas of improvements, and results of the re-engineering process. The conversion from the M ampersand O to the M ampersand I contract concept resulted in multiple independent Rad Con organizations reporting to separate major contractors who are managed by an integrating contractor. This brought significant challenges when establishing minimum site standards for sitewide consistency, developing roles and responsibilities, and maintaining site Rad Con goals. Championed by the previous contractor's Rad Con Director, Denny Newland, a five month planning effort was executed to address the challenges of the M ampersand I and to address identified weaknesses. Fluor Daniel Hanford assumed the responsibility as integrator of the Project Hanford Management Contract on October 1, 1996. The Fluor Daniel Hanford Radiation Protection Director Jeff Foster presents the results of the re-engineering effort, including the significant cost savings, process improvements, field support improvements, and clarification of roles and responsibilities that have been achieved

["Mixed" and "miscellaneous" vulvovaginitis: diagnostics and therapy of vaginal administration of nystatin and nifuratel].

Science.gov (United States)

Cepický, P; Malina, J; Líbalová, Z; Kuzelová, M

2005-05-01

The evaluation of combined and miscellaneous vulvovaginal infections incidence and their treatment with combined vaginal products containing nifuratel and nystatin. Prospective study. Gynecologic outpatient department LEVRET, Prague; Laboratories of Microbiology AescuLab, Prague. 70 consecutive patients were examined with complaint of vaginal fluor and/or pruritus. We established macroscopic features of fluor, pH, amine test and mounts stained with Giemsa and Gram. We qualified the cases with more diagnostic criteria (mycosis, lactobacillosis, anaerobic vaginosis, aerobic vaginitis) as combined infection, those with no diagnostic criteria as miscellaneous. We treated all patients with vaginal tablets nystatin + nifuratel (Macmiror complex). We prescribed clotrimazol cream, if pruritus was present. We evaluated withdrawals of symptoms and relapses during 3 months after treatment. Combined infection was found in 21 patients from 70 (30%). The most frequent combination was that of mycosis and aerobic vaginitis (13/70, 18.6%) or mycosis and anaerobic vaginosis (4/70, 5.7%); 11 patients fulfilled criteria of no diagnosis. We concluded them as "miscelaneous". The treatment was successful in all cases, 10 women relapsed in 3 months. Combined vaginal infection findings are present very often (30%), likewise miscellaneous ones (15%) occur. The treatment of these women in successful with vaginal tablets with nystatin + nifuratel.

Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food

Directory of Open Access Journals (Sweden)

Arun K. Bhunia

2009-07-01

Full Text Available Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11 was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods.

Overview on the history of organofluorine chemistry from the viewpoint of material industry

OpenAIRE

Okazoe, Takashi

2009-01-01

Fluorine (from ?le fluor?, meaning ?to flow?) is a second row element of Group 17 in the periodic table. When bound to carbon it forms the strongest bond in organic chemistry to give organofluorine compounds. The scientific field treating them, organofluorine chemistry, started before elemental fluorine itself was isolated. Applying the fruits in academia, industrial organofluorine chemistry has developed over 80 years via dramatic changes during World War II. Nowadays, it provides various ma...

SITUATION OF FLUORIDES RATE IN WATERS AND MAJOR ...

African Journals Online (AJOL)

31 déc. 2010 ... by fluoride ions in the waters of the region (Fluorosis Dental and skeletal). This study proposes, the estimated daily intake of fluoride from its water .... Le fluor est dosé par la méthode potentiométrique (NF T 90-004) grâce à une électrode spécifique aux ions fluorures. La mesure du potentiel a été effectuée ...

Heat-resistant hydrophobic-oleophobic coatings

OpenAIRE

Uyanik, Mehmet; Arpac, Ertugrul; Schmidt, Helmut K.; Akarsu, Murat; Sayilkan, Funda; Sayilkan, Hikmet

2006-01-01

Thermally and chemically durable hydrophobic oleophobic coatings, containing different ceramic particles such as SiO2, SiC, Al 2O3, which can be alternative instead of Teflon, have been developed and applied on the aluminum substrates by spin-coating method. Polyimides, which are high-thermal resistant heteroaromatic polymers, were synthesized, and fluor oligomers were added to these polymers to obtain hydrophobic-oleophobic properties. After coating, Al surface was subjected to Taber-abrasio...

Sensitivity of Breast Cancer Stem Cells to TRA-8 Anti-DR5 Monoclonal Antibody

Science.gov (United States)

2013-08-01

Janelia Farm , VA, travel award 2010 -­‐ Translational Medicine Symposium, HHMI Peer Cluster Meeting, travel award 2010 -­‐ AACR Annual Conference...lab. Science Education Alliance, Janelia Farm , VA, 2001 3. Londoño-Joshi AI, Forero-Torres A, Fineberg NS, Oliver PG, Zhou T, LoBuglio AF, Buchsbaum...rabbit mAb and cleaved caspase 3 rabbit mAb were purchased from Cell Signaling (Billerica, MA). Secondary antibodies, Alexa fluor 405 goat anti-rabbit

Porous silicon: some new applications of this material with astonishing properties; Le silicium poreux: nouvelles applications de ce materiau aux proprietes remarquables

Energy Technology Data Exchange (ETDEWEB)

Gauthier-Manuel, B. [Laboratoire FEMTO-ST, Dept. MN2S, CNRS UMR 6174, 25 - Besancon (France)

2009-02-15

Anodic oxidation of silicon wafer in fluor-hydric acid solution leads to a porous material with surprising properties. Structures at nano-metric scale are source of deep photoluminescence. The very large scale of pore size available and the high specific surface obtained allows optical and microfabrication applications. This paper describes the electrochemical process of this material, some characterization methods, and some new applications in the fields of micro-energy and molecular separation. (author)

Minutes of the Explosives Safety Seminar (25). Volume 4. Held in Anaheim, California on 18-20 Aug 1992

Science.gov (United States)

1992-08-01

7 Mr. Colin McMillan, Assistant Secretary of Defense for Production and Logistics NAVY SA FETY...McMahon, Gordon W. 11-277 Song, So-Young 1-429, 11-85 McMillan, Colin 1-7 Spivey, Kathy H. 11-547 McQueen, D. IV-203 Starkenberg, John 1-183 McVay, L. IV...FLUOR DANIEL MR. DRURY CHUCK SHAMROCK SERVICES MR. DUA BALBIR DAY & ZIMMERMANN, INC. MR. DUCHOCK JEFF MR. DUDLEY MICHAEL DEFENSE LOGISTICS AGENCY

Mid-Infrared Fiber Lasers (Les fibres laser infrarouge moyen)

Science.gov (United States)

2010-09-01

Marcel Poulain, Université de Rennes / Le Verre Fluoré, France Fluoride Fiber Sources: Problems and Prospects Prof. Marcel Poulain from Rennes...currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 2. REPORT TYPE 3. DATES...Les fibres laser infrarouge moyen (RTO-MP-SET-171) Synthèse Les sources laser en infrarouge moyen sont nécessaires aux systèmes actifs de

Bright photoactivatable fluorophores for single-molecule imaging.

Science.gov (United States)

Grimm, Jonathan B; English, Brian P; Choi, Heejun; Muthusamy, Anand K; Mehl, Brian P; Dong, Peng; Brown, Timothy A; Lippincott-Schwartz, Jennifer; Liu, Zhe; Lionnet, Timothée; Lavis, Luke D

2016-12-01

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

Safety Evaluation for Packaging (onsite) T Plant Canyon Items

International Nuclear Information System (INIS)

OBRIEN, J.H.

2000-01-01

This safety evaluation for packaging (SEP) evaluates and documents the ability to safely ship mostly unique inventories of miscellaneous T Plant canyon waste items (T-P Items) encountered during the canyon deck clean off campaign. In addition, this SEP addresses contaminated items and material that may be shipped in a strong tight package (STP). The shipments meet the criteria for onsite shipments as specified by Fluor Hanford in HNF-PRO-154, Responsibilities and Procedures for all Hazardous Material Shipments

QUEST Hanford Site Computer Users - What do they do?

Energy Technology Data Exchange (ETDEWEB)

WITHERSPOON, T.T.

2000-03-02

The Fluor Hanford Chief Information Office requested that a computer-user survey be conducted to determine the user's dependence on the computer and its importance to their ability to accomplish their work. Daily use trends and future needs of Hanford Site personal computer (PC) users was also to be defined. A primary objective was to use the data to determine how budgets should be focused toward providing those services that are truly needed by the users.

2008-IJBCS-Article Kalpy Julien Coulybaly

African Journals Online (AJOL)

hp

éclairée par 4 ampoules néon MAZDA. FLUOR M2TF 36W/LJ de 1,2 m de long, de. 32 Watt produisant une lumière type lumière du jour de 3350 lumens chacun. D'où une intensité lumineuse globale de 706,66 lux. Une seconde expérience avec une légère modification de la composition des milieux. Bold et Conways, une ...

Center for the Evaluation of Biomarkers for the Early Detection of Breast Cancer

Science.gov (United States)

2009-10-01

Table S1, including mammography (BI-RADS) score and breast density at the last mammogram before diagnosis, lymph node positivity, tumor size, number of...Bibliography 1.& Fletcher RH. Should all people over the age of 50 have regular fecal occult-blood tests? If it works, why not do it? New England Journal...blocked for 15 minutes at room temperature in fluores- cence-activated cell sorting (FACS) buffer (PBS, 0.1% bovine serum albumin (BSA), 0.02% sodium

Safety Evaluation for Packaging (onsite) T Plant Canyon Items

Energy Technology Data Exchange (ETDEWEB)

OBRIEN, J.H.

2000-07-14

This safety evaluation for packaging (SEP) evaluates and documents the ability to safely ship mostly unique inventories of miscellaneous T Plant canyon waste items (T-P Items) encountered during the canyon deck clean off campaign. In addition, this SEP addresses contaminated items and material that may be shipped in a strong tight package (STP). The shipments meet the criteria for onsite shipments as specified by Fluor Hanford in HNF-PRO-154, Responsibilities and Procedures for all Hazardous Material Shipments.

The Seven Brothers. The changing world of petrochemical multinationals

International Nuclear Information System (INIS)

Van Ammelrooij, A.

2000-01-01

Since the eighties oil companies do not build their own drilling installations and refineries, but contract out such construction activities to engineering contractors. An overview is given of the market of the so-called Seven Brother (analogue to Seven Sisters for the oil companies) and the changes that are taking place at the moment. The Seven Brothers are Asea Brown Boveri (USA), Fluor Corp (US), Kvaerner ASA (Norway), Foster Wheeler (UK), Jacobs (USA), Morrison Knudsen (USA), and Parsons (UK)

The use of selective electrodes for the control of nuclear materials

International Nuclear Information System (INIS)

Pires, M.A.F.; Abrao, A.

1984-01-01

The use of ion selective electrodes is discussed for the determination of nitrate, chloride and fluoride in several materials used in the fuel cycle. The determination of nitrate and chloride in thorium compounds, the analysis of fluoride and chloride in uranium compounds and the determination of fluoride in crude phosphoric acid are described. The control of fluoride in urine of individuals that handle materials containing fluor and its compounds is also described. (C.L.B.) [pt

Fluor-Hanford 3013 Digital Radiography Dead Zone Mitigation Project Pressure Test Report

International Nuclear Information System (INIS)

Gibbs, K.

2003-01-01

The use of digital radiographic (DR) measurement of lid deflection as an indication of pressurization of the 3013 inner can was first reported by the Savannah River Technology Center (SRTC). The conclusions of this report were that for cans with relatively large initial concavity, lid deflection could be used to meet the 3013 standard (DOE-STD-3013-2000) requirement for a nondestructive indication of a pressurization of 100 psig. During acceptance testing of the system in the Spring of 2003, it was confirmed that for some cans the DR measured lid deflection could become insensitive to the change in lid deflection when compared to actual mechanical measurements. The basic explanation of this phenomenon is that characteristics of the lid geometry such as tilt and wobble can obfuscate the bottom of the lid where the deflection is measured. The purpose of this report is to document the results of the pressure testing and the efficacy of the alternate imaging and analysis methods developed to mitigate the dead zone problem. Prior to review of the results, a review of the current method and an introduction to the newly developed methods and techniques is provided

Clinical assessment of demineralization and remineralization surrounding orthodontic brackets with FluoreCam

Directory of Open Access Journals (Sweden)

Bora Korkut

2017-04-01

Conclusions: This study demonstrated that demineralization is measurable around orthodontic brackets and the demineralization can be completely inhibited and/or reversed by the use of commercially available remineralization products.

Marrying project deliverability models and labour supply for the oil sands

Energy Technology Data Exchange (ETDEWEB)

Albright, R.; Whitaker, C.A. [Fluor Canada Ltd., Calgary, AB (Canada)

2005-07-01

Alberta is predicting a labour shortage to support the construction and operation of planned oil sands projects in the region. This paper provided a framework of options available to stakeholders in addressing resource constraints from the viewpoint of Fluor Canada Ltd. A background of previous projects was presented, which highlighted various strategies used in the management of human resources issues. Strategies included local hiring through the Alberta-based trade unions; recruitment from other Canadian provinces; a higher ratio of apprentices; extremely high utilization crew schedules; and extended use of overtime. It was noted that these strategies resulted in some cost overruns and lower productivity levels. A productivity analysis was used to determine and resolve productivity issues. Various regulations in Alberta were discussed in relation to training programs and skilled immigrants. Various international training facilities operated by Fluor were reviewed. A factor model of human resources issues was presented. Summaries of supply chain management, systems, and technologies were presented. Issues concerning prefabrication, pre-assembly, modularization and offsite fabrication were discussed. It was concluded that by taking advantage of lessons learned in previous projects a solid foundation is set from which to plan for future requirements. Successful project execution is achieved by taking advantage of current technology; enhancing modular construction standards; and using supply chain management techniques and enhanced labour supply solutions. tabs., figs.

DESIGN OF A SYSTEM TO RETRIEVE SLUDGE FROM THE K EAST SPENT FUEL BASIN AT HANFORD

International Nuclear Information System (INIS)

Twitchell, A.L.; MacLean, G.T.; Ho, Q.T.; Fort, D.L.

2003-01-01

This paper describes the Sludge Retrieval System (SRS), which was designed to safely remove radioactive sludge from the K East spent fuel basin at the 100 K Area of the Hanford Site. Basin water and sludge have the potential to leak to the environment due to the age and condition of the basins. Since the 100 K Area spent fuel basins are located next to the Columbia River, the Spent Nuclear Fuel Project mission includes the safe removal, containment, and transportation of sludge from the basins to a secure storage location. The scope of the SRS includes: A system capable of retrieving sludge from the K East basin floor, pits, and fuel canisters; Separation of debris from sludge, where debris is defined as any material greater than 0.64 cm (0.25 in.) in diameter; Collection of sludge particles in a container that can be transported away from the basin; Modifications to the K East basin to allow installation of the SRS. The SRS was designed by Fluor Federal Services. Changes to the designed system were made by Fluor Hanford as a result of full-scale testing performed after design. This paper discusses this testing, as well as operation and control of the system. Construction and startup testing was initially scheduled to be complete by the end of December 2002. Startup of the system is now expected in April 2003

Effect of different concentrations of fluoride varnish on enamel surface microhardness: An in vitro randomized controlled study

Directory of Open Access Journals (Sweden)

Priya Subramaniam

2016-01-01

Full Text Available Introduction: Dental caries occurs as a result of demineralization-remineralization phases occurring alternately at the tooth surface. Fluoride varnishes have a caries-inhibiting effect on teeth through remineralization. The resulting enamel is resistant to acid dissolution. Aim: The aim of this study is to assess enamel surface microhardness (SMH following varnish application with different fluoride concentrations. Materials and Methods: Ninety freshly extracted, caries-free premolar teeth were used. Teeth were sectioned to obtain enamel blocks from the buccal surface of crown. The blocks were serially polished and flattened, embedded in acrylic blocks and smoothened to achieve a flat surface. The samples were divided into three groups, namely, A, B, and C consisting of 30 enamel blocks each. In Group A, Fluor Protector® varnish and in Group B, Bi-Fluorid 10® varnish was applied. Group C served as controls. All samples were subjected to a demineralization-remineralization cycle for 7 days. The SMH of enamel was measured. Data obtained was subjected to statistical analysis using the Student's t-test and one-way ANOVA. Results: The mean values of enamel SMH of Groups A and B were 496.99 ± 4.81 and 449.47 ± 7.37 Vickers Hardness Number, respectively. Conclusion: Fluor Protector varnish showed significantly higher enamel SMH than that of the other two groups (P < 0.05.

Repowering analysis: Hanford Generating Project (HGP), Task Order Number 6

International Nuclear Information System (INIS)

1988-12-01

The Hanford Generating Project (HGP), owned by the Washington Public Power Supply System, consists of two low pressure steam turbines, generators, and associated equipment located adjacent to the Department of Energy's (DOE) N-Reactor. HGP has been able to produce approximately 800 MWe with low pressure steam supplied by N-Reactor. DOE has placed N-Reactor in cold standby status for an undetermined length of time. This results in the idling of the HGP since no alternative source of steam is available. Bonneville Power Administration contracted with Fluor Daniel, Inc. to investigate the feasibility and cost of constructing a new source of steam for (repowering) one of the HGP turbines. The steam turbine is currently operated with 135 psia steam. The turbines can be rebuilt to operate with 500 psia steam pressure by adding additional stages, buckets, nozzles, and diaphragms. Because of the low pressure design, this turbine can never achieve the efficiencies possible in new high pressure turbines by the presences of existing equipment reduces the capital cost of a new generating resource. Five repowering options were investigated in this study. Three cases utilizing gas turbine combined cycle steam generation equipment, one case utilizing a gas fired boiler, and a case utilizing a coal fired boiler. This report presents Fluor Daniel's analysis of these repowering options

HWVP submerged bed scrubber waste treatment by ion exchange at high pH

International Nuclear Information System (INIS)

Bray, L.A.; Carson, K.J.; Elovich, R.J.; Eakin, D.E.

1996-03-01

The Hanford Waste Vitrification Plant (HWVP) is expected to produce aqueous waste streams that will require further processing for cesium, strontium, and transuranic (TRU) removal prior to incorporation into grout. Fluor Daniel, Inc. has recommended that zeolite be added to these waste streams for adsorption of cesium (Cs) and strontium (Sr) following pH adjustment by sodium hydroxide (NAOH) addition. Filtration will then used to remove the TRU elements associated with the process solids and the zeolite containing the Cs and Sr

HWVP submerged bed scrubber waste treatment by ion exchange at high pH

Energy Technology Data Exchange (ETDEWEB)

Bray, L.A.; Carson, K.J.; Elovich, R.J.; Eakin, D.E.

1996-03-01

The Hanford Waste Vitrification Plant (HWVP) is expected to produce aqueous waste streams that will require further processing for cesium, strontium, and transuranic (TRU) removal prior to incorporation into grout. Fluor Daniel, Inc. has recommended that zeolite be added to these waste streams for adsorption of cesium (Cs) and strontium (Sr) following pH adjustment by sodium hydroxide (NAOH) addition. Filtration will then used to remove the TRU elements associated with the process solids and the zeolite containing the Cs and Sr.

PALS investigations of free volumes thermal expansion of J-PET plastic scintillator synthesized in polystyrene matrix

Directory of Open Access Journals (Sweden)

Wieczorek Anna

2015-12-01

Full Text Available The polystyrene doped with 2,5-diphenyloxazole as a primary fluor and 2-(4-styrylphenylbenzoxazole as a wavelength shifter prepared as a plastic scintillator was investigated using positronium probe in wide range of temperatures from 123 to 423 K. Three structural transitions at 260, 283, and 370 K were found in the material. In the o-Ps intensity dependence on temperature, the significant hysteresis is observed. Heated to 370 K, the material exhibits the o-Ps intensity variations in time.

Uranium deposit of Bauzot (Saone et Loire)

International Nuclear Information System (INIS)

Carrat, G.H.

1956-01-01

The best known of the uranium ore deposits of the Morvan (a province of France) is in the form of a bundle of quartz-fluor lodes with pitchblende and B.P.G.C. ore. The pitchblende seems to have been deposited at different time in respect to the formation of the gangue minerals, but generally it is ore of the first-formed. The main concentrations of ore are always in the vicinity of dykes of basic crystalline rocks. (author) [fr

Evaluation and Assessment of Fluoride in Drinking Water Wells Damavand Villages Zoning in GIS According to DMF Index

OpenAIRE

Kave Kheirkhah Rahimabad; Amir Hessam Hasani; Reza Saeedi; Mir Masoud Kheirkhah Zarkesh; Mojtaba Sayadi

2016-01-01

Background:Fluoride is one the vital anions and the drinking water is the main source of preparing it for the human body. Nonetheless, the aim of this paper is to investigate the Fluoride rate in water supplying wells by using GIS environment according to decay, missing or filled (DMF) index.  Methods: This research is an analytic and cross-sectional descriptive study with sampling approach of 12 water supplying wells of Damavand villages in summer and autumn the year 2013. The Fluor...

Immobilized low-level waste disposal options configuration study

International Nuclear Information System (INIS)

Mitchell, D.E.

1995-02-01

This report compiles information that supports the eventual conceptual and definitive design of a disposal facility for immobilized low-level waste. The report includes the results of a joint Westinghouse/Fluor Daniel Inc. evaluation of trade-offs for glass manufacturing and product (waste form) disposal. Though recommendations for the preferred manufacturing and disposal option for low-level waste are outside the scope of this document, relative ranking as applied to facility complexity, safety, remote operation concepts and ease of retrieval are addressed

Label Free Chromosome Translocation Detection with Silicon nanowires

DEFF Research Database (Denmark)

Kwasny, Dorota; Andersen, Karsten Brandt; Frøhling, Kasper Bayer

HROMOSOME translocation, which is a rearrangement of arms between two chromosomes, is a major group of chromosome abnormalities leading to cancer. As a result, two derivative chromosomes with sequences coming from both chromosomes are formed. The current translocation detection method is a Fluore......HROMOSOME translocation, which is a rearrangement of arms between two chromosomes, is a major group of chromosome abnormalities leading to cancer. As a result, two derivative chromosomes with sequences coming from both chromosomes are formed. The current translocation detection method...

Apparent light requirement for activation of photosynthesis upon rehydration of desiccated beachrock microbial mats

DEFF Research Database (Denmark)

Schreiber, Ulrich; Gademann, Rolf; Bird, Paul

2002-01-01

. Parallel measurements of O2 concentration with an oxygen microoptode revealed zero oxygen concentration in the surface layer of rehydrated beachrock in the dark. Upon illumination, O2 concentration increased in parallel with PSII quantum yield and decreased again to zero in the dark. It is proposed......, emitter-detector unit; Fo, fluor-escence yield of dark-adapted sample; Fm, maximal fluorescence yield measured during saturation pulse; Fv, variable fluorescence yield; LED, light-emitting diode; PAM, pulse amplitude modulation; PQ, plastoquinone...

Recovery of fluoride ion selective electrode

International Nuclear Information System (INIS)

Monteiro, R.P.G.

1988-01-01

A recovery procedure of fluoride ion selective electrode based upon the body radiography of inactive electrode and introduction of suitable internal regeneration solution, is developed. The recovered electrode was tested in standard solutions of fluoride ions (10 sup5) to 10 -1M showing as good performance as the new one. The fluor determination by potentiometric measurements with selective electrode is used in nuclear fuel cycle for quality control of thorium and uranium mixed oxide pellets and pellets of uranium dioxides. (author) [pt

User Manual for the AZ-101 Data Acquisition System (AS-101 DAS)

Energy Technology Data Exchange (ETDEWEB)

BRAYTON, D.D.

2000-02-17

User manual for the TK AZ-101 Waste Retrieval System Data Acquisition System. The purpose of this document is to describe use of the AZ-101 Data Acquisition System (AZ-101 DAS). The AZ-101 DAS is provided to fulfill the requirements for data collection and monitoring as defined in Letters of Instruction (LOI) from Numatec Hanford Corporation (NHC) to Fluor Federal Services (FFS). For a complete description of the system, including design, please refer to the AZ-101 DAS System Description document, RPP-5572.

User Manual for the AZ-101 Data Acquisition System (AS-101 DAS)

International Nuclear Information System (INIS)

BRAYTON, D.D.

2000-01-01

User manual for the TK AZ-101 Waste Retrieval System Data Acquisition System. The purpose of this document is to describe use of the AZ-101 Data Acquisition System (AZ-101 DAS). The AZ-101 DAS is provided to fulfill the requirements for data collection and monitoring as defined in Letters of Instruction (LOI) from Numatec Hanford Corporation (NHC) to Fluor Federal Services (FFS). For a complete description of the system, including design, please refer to the AZ-101 DAS System Description document, RPP-5572

Resistência à microtração entre uma cerâmica hidrotérmica, silanizada ou não, aderida à uma resina composta por um cimento resinoso

OpenAIRE

Lopes, Angela; Leite, Fabiola; Valandro, Luis Felipe; Siqueira, Luciana; Pimenta, Luiz André; Neisser, Maximiliano Piero

2010-01-01

Esse trabalho teve como objetivo avaliar a resistência adesiva entre uma cerâmica hidrotérmica (Symbio ceram, Degussa), silanizada ou não e aderida à uma resina composta (Filtec Z100- 3M ESPE) por um cimento resinoso (Panavia F, Kuraray). Foram confeccionados 2 blocos, divididos em 2 grupos assim constituídos: Grupo I-tratamento de superfície da cerâmica com ácido fluorídrico a 10% e união a um substrato (resina compost...

Qualification for Safeguards Purposes of UF6 Sampling using Alumina – Results of the Evaluation Campaign of ABACC-Cristallini Method

OpenAIRE

ESTAEBAN ADOLFO; GAUTIER EDUARDO; MACHADO DA SILVA LUIS; FERNANDEZ MORENO SONIA; RENHA JR GERALDO; DIAS FABIO; PEREIRA DE OLIVEIRA JUNIOR OLIVIO; AMMARAGGI DAVID; MASON PETER; SORIANO MICHAEL; CROATTO PAUL; ZULEGER EVELYN; GIAQUINTO JOSEPH; HEXEL COLE; VERCOUTER THOMAS

2017-01-01

The procedure currently used to sample material from process lines in uranium enrichment plants consists of collecting the uranium hexafluoride (UF6) in gaseous phase by desublimation inside a metal sampling cylinder cooled with liquid nitrogen or in certain facilities in a fluorothene P-10 tube type. The ABACC-Cristallini method (A-C method) has been proposed to collect the UF6 (gas) by adsorption in alumina (Al2O3) in the form of uranyl fluoride (UO2F2) (solid). This method uses a fluor...

Preparation of plutonium hexafluoride. Recovery of plutonium from waste dross (1962); Preparation de l'hexafluorure de plutonium. Recuperation du plutonium des scories d'elaboration (1962)

Energy Technology Data Exchange (ETDEWEB)

Gendre, R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1962-07-01

facteurs physiques sur la vitesse de fluoration du tetrafluorure de plutonium solide par le fluor. Dans un appareil a four horizontal et circulation de fluor a la pression atmospherique de 520 deg. C, avec un debit de fluor pur de 9 l.h{sup -1}, il est possible de transformer une charge de 3 g de tetrafluorure en hexafluorure avec un taux de transformation d'environ 100 pour cent et un rendement de recuperation superieur a 90 pour cent, en 4 a 5 h. La vitesse de fluoration est fonction de la temperature du debit de fluor, de la surface de la nacelle, de l'epaisseur de la couche de tetrafluorure et du temps de reaction. Elle ne depend pas de la diffusion du fluor dans le Solide mais est commandee par la reaction a l'interface gaz-solide et suit la loi cinetique (1 - T{sub T}){sup 1/3} = kt + 1. L'existence de fluorures intermediaires, en particulier Pu{sub 4} F{sub 17}, est confirmee par une cassure de la droite d'Arrhenius vers 370 deg. C, par des differences dans les vitesses de fluoration au sein de la couche de tetrafluorure et par des variations reversibles de coloration. La transformation en hexafluorure s'accompagne d'une purification vis-a-vis des elements etrangers entrant dans la composition de plutonium initial. Recuperation du plutonium des scories d'elaboration: L'etude est basee sur la transformation en hexafluorure gazeux des grains de plutonium occlus dans les scories d'elaboration, cet hexafluorure devant fournir par decomposition thermique un tetrafluorure directement recyclable au stade elaboration. - Dans les conditions envisagees, ce procede n'est pas utilisable industriellement. Apres broyage, une Reparation par milieu dense (iodure de methylene) permet de separer une scorie enrichie en plutonium (75 pour cent du Pu dans 2,6 pour cent du poids des scories) et une scorie pauvre. Par fluoration prolongee (16 h) des diverses portions recueillies, il est possible de recuperer environ 80 pour cent du plutonium. Un appareillage de traitement base sur la

Integration of health physics, safety and operational processes for management and disposition of recycled uranium wastes at the Fernald Environmental Management Project (FEMP)

International Nuclear Information System (INIS)

Barber, James; Buckley, James

2003-01-01

Fluor Fernald, Inc. (Fluor Fernald), the contractor for the U. S. Department of Energy (DOE) Fernald Environmental Management Project (FEMP), recently submitted a new baseline plan for achieving site closure by the end of calendar year 2006. This plan was submitted at DOE's request, as the FEMP was selected as one of the sites for their accelerated closure initiative. In accordance with the accelerated baseline, the FEMP Waste Management Project (WMP) is actively evaluating innovative processes for the management and disposition of low-level uranium, fissile material, and thorium, all of which have been classified as waste. These activities are being conducted by the Low Level Waste (LLW) and Uranium Waste Disposition (UWD) projects. Alternatives associated with operational processing of individual waste streams, each of which poses potentially unique health physics, industrial hygiene and industrial hazards, are being evaluated for determination of the most cost effective and safe met hod for handling and disposition. Low-level Mixed Waste (LLMW) projects are not addressed in this paper. This paper summarizes historical uranium recycling programs and resultant trace quantity contamination of uranium waste streams with radionuclides, other than uranium. The presentation then describes how waste characterization data is reviewed for radiological and/or chemical hazards and exposure mitigation techniques, in conjunction with proposed operations for handling and disposition. The final part of the presentation consists of an overview of recent operations within LLW and UWD project dispositions, which have been safely completed, and a description of several current operations

Design of a System to Retrieve Sludge from the K East Spent Fuel Basin at Hanford

International Nuclear Information System (INIS)

TWITCHELL, A.L.

2003-01-01

This paper describes the Sludge Retrieval System (SRS), which was designed to safely remove radioactive sludge from the K East spent fuel basin at the 100 K Area of the Hanford Site. Basin water and sludge have the potential to leak to the environment due to the age and condition of the basins. Since the 100 K Area spent fuel basins are located next to the Columbia River, the Spent Nuclear Fuel Project mission includes the safe removal, containment, and transportation of sludge from the basins to a secure storage location. The scope of the SRS includes: (1) a system capable of retrieving sludge from the K East basin floor, pits, and fuel canisters; (2) separation of debris from sludge, where debris is defined as any material greater than 0.64 cm (0.25 in.) in diameter; (3) collection of sludge particles in a container that can be transported away from the basin; and (4) modifications to the K East basin to allow installation of the SRS. The SRS was designed by Fluor Federal Services. Changes to the designed system were made by Fluor Hanford as a result of full-scale testing performed after design. This paper discusses this testing, as well as operation and control of the system. Construction and startup testing was initially scheduled to be complete by the end of December 2002. Startup of the system is now expected in April 2003

Anticalculus efficacy of a new dentifrice.

Science.gov (United States)

Llena, Carmen; Forner, Leopoldo; Vento, Cristina

2009-06-01

The Volpe and Manhold Index (VMI) was used to evaluate the anticalculus efficacy of the dentifrice Fluor Kin antisarro, used on adults twice a day for 2 minutes over 3 months, in comparison with the dentifrice Lacer. A clinical study in which 48 adults took part was designed. The presence of calculus was determined at baseline (VMI), a tartrectomy was performed, and the patients were instructed to brush their teeth twice a day for 2 minutes, using a medium-bristled toothbrush with a commercial dentifrice containing 0.2% aldioxa and 1.9% sodium monofluorophosphate. Three months later, VMI was determined again. After 2 weeks, a new tartrectomy was performed on the same patients, and the subjects were instructed to brush their teeth as before, this time with the study dentifrice based on 0.32% sodium fluoride, 4.1% tetrapotassium pyrophosphate, 2.0% PVM/MA (methylvinylether/maleic acid) copolymer (Gantrez S), 1.0% provitamin B5, and 0.2% vitamin B3. After an additional 3 months, dental calculus was determined again. The study dentifrice showed a significant reduction in VMI in relation to the initial situation (P = .009). There were also significant differences in VMI values between the 2 dentifrices (P = .010). VMI differences were not significant for either dentifrice when analyzed by gender, tobacco use, and toothbrushing frequency before starting the study. Brushing twice a day for 2 minutes with Fluor Kin antisarro effectively reduces supragingival calculus.

A fluorescence resonance energy transfer-based approach for investigating late endosome-lysosome retrograde fusion events.

Science.gov (United States)

Kaufmann, A M; Goldman, S D B; Krise, J P

2009-03-01

Traditionally, lysosomes have been considered to be a terminal endocytic compartment. Recent studies suggest that lysosomes are quite dynamic, being able to fuse with other late endocytic compartments as well as with the plasma membrane. Here we describe a quantitative fluorescence energy transfer (FRET)-based method for assessing rates of retrograde fusion between terminal lysosomes and late endosomes in living cells. Late endosomes were specifically labeled with 800-nm latex beads that were conjugated with streptavidin and Alexa Fluor 555 (FRET donor). Terminal lysosomes were specifically labeled with 10,000-MW dextran polymers conjugated with biotin and Alexa Fluor 647 (FRET acceptor). Following late endosome-lysosome fusion, the strong binding affinity between streptavidin and biotin brought the donor and acceptor fluorophore molecules into close proximity, thereby facilitating the appearance of a FRET emission signal. Because apparent size restrictions in the endocytic pathway do not permit endocytosed latex beads from reaching terminal lysosomes in an anterograde fashion, the appearance of the FRET signal is consistent with retrograde transport of lysosomal cargo back to late endosomes. We assessed the efficiency of this transport step in fibroblasts affected by different lysosome storage disorders-Niemann-Pick type C, mucolipidosis type IV, and Sandhoff's disease, all of which have a similar lysosomal lipid accumulation phenotype. We report here, for the first time, that these disorders can be distinguished by their rate of transfer of lysosome cargos to late endosomes, and we discuss the implications of these findings for developing new therapeutic strategies.

Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-β Exposes a Gβγ Protein Binding Site*

OpenAIRE

Kadamur, Ganesh; Ross, Elliott M.

2016-01-01

Mammalian phospholipase C-β (PLC-β) isoforms are stimulated by heterotrimeric G protein subunits and members of the Rho GTPase family of small G proteins. Although recent structural studies showed how Gαq and Rac1 bind PLC-β, there is a lack of consensus regarding the Gβγ binding site in PLC-β. Using FRET between cerulean fluorescent protein-labeled Gβγ and the Alexa Fluor 594-labeled PLC-β pleckstrin homology (PH) domain, we demonstrate that the PH domain is the minimal Gβγ binding region in...

Fiber optic neutron imaging system: calibration

International Nuclear Information System (INIS)

Malone, R.M.; Gow, C.E.; Thayer, D.R.

1981-01-01

Two neutron imaging experiments using fiber optics have been performed at the Nevada Test Site. In each experiment, an array of scintillator fluor tubes is exposed to neutrons. Light is coupled out through radiation resistant PCS fibers (8-m long) into high-bandwidth, graded index fibers. For image reconstruction to be accurate, common timing differences and transmission variations between fiber optic channels are needed. The calibration system featured a scanning pulsed dye laser, a specially designed fiber optic star coupler, a tektronix 7912AD transient digitizer, and a DEC PDP 11/34 computing system

Canister storage building trade study. Final report

International Nuclear Information System (INIS)

Swenson, C.E.

1995-05-01

This study was performed to evaluate the impact of several technical issues related to the usage of the Canister Storage Building (CSB) to safely stage and store N-Reactor spent fuel currently located at K-Basin 100KW and 100KE. Each technical issue formed the basis for an individual trade study used to develop the ROM cost and schedule estimates. The study used concept 2D from the Fluor prepared ''Staging and Storage Facility (SSF) Feasibility Report'' as the basis for development of the individual trade studies

Canister storage building trade study. Final report

Energy Technology Data Exchange (ETDEWEB)

Swenson, C.E. [Westinghouse Hanford Co., Richland, WA (United States)

1995-05-01

This study was performed to evaluate the impact of several technical issues related to the usage of the Canister Storage Building (CSB) to safely stage and store N-Reactor spent fuel currently located at K-Basin 100KW and 100KE. Each technical issue formed the basis for an individual trade study used to develop the ROM cost and schedule estimates. The study used concept 2D from the Fluor prepared ``Staging and Storage Facility (SSF) Feasibility Report`` as the basis for development of the individual trade studies.

Developments in the LASL Fuel Pin Imaging System: PINEX-3A

International Nuclear Information System (INIS)

Lumpkin, A.H.; Berzins, G.J.; Cosimi, R.A.; O'Hare, T.E.; Davidson, J.R.

1979-01-01

The LASL Fuel Pin Imaging System was evaluated using a series of 10 TREAT transients, each of approx. 240-MW peak power. HEDL provided the fuel-ejection type capsule with annular fuel pellets. The pin visibility threshold was determined to be approx. 20-MW of TREAT power (approx. 130 W/g), almost an order of magnitude improvement over our PINEX-2 threshold. The impact of changes in instrumentation, imaging apertures, and fluors that produced the improved sensitivity are reported. Results of a time-integrated imaging technique are also presented

Influencia de los tratamientos microabrasivos sobre la resistencia adhesiva en dientes con fluorosis

OpenAIRE

Paola Mena Silva; Ana del Carmen Armas

2015-01-01

La búsqueda de tratamientos mínimamente invasivos y altamente estéticos para pacientes con fluorosis, ha llevado a buscar mecanismos para solucionar esta problemática, mediante la utilización de resinas como la primera opción. Sin embargo el principal problema del tratamiento, se centra en la poca adhesión debido a las características estructurales del esmalte fluorótico, en grados de fluorosis TF 1 al 3, el ácido no actúa correctamente, causando ausencia de grabado principalmente entre las e...

Utility of FDG-PET in neuropediatric pathology; Utilidad del PET-FDG en patologia neuropediatrica

Energy Technology Data Exchange (ETDEWEB)

Massardo, Teresa V; Canessa G, Jose; Sierralta C, M. Paulina; Gonzalez E, Pstricio; Jofre M, M. Josefina [Centro PET Imagenes Moleculares Hospital Militar de Santiago (Chile)

2007-07-01

Due to the recent incorporation of positron technology (PET) it appears interesting to review the present indications of fluor-deoxyglucose (FDG) in neuropediatric pathology. The main indication is in the evaluation of refractory epilepsy mainly in cases with negative magnetic resonance or discordance with electroencephalography Some uses in tumors are also discussed, such as tumor characterization and differentiation between radionecrosis and recurrence. The experience between years 2003 and 2007 at the Santiago Military Hospital, using FDG in 66 children and adolescents cases with diverse neurological indications was reviewed.

Usefulness of PET-FDG in pathology neuropediatric; Utilidad del PET-FDG en patologia neuropediatrica

Energy Technology Data Exchange (ETDEWEB)

Massardo V, Teresa; Canessa G, Jose; Sierralta C, M Paulina; Gonzalez E, Patricio; Jofre M, M Josefina [Centro PET Imagenes Moleculares Hospital Militar de Santiago, Santiago (Chile)

2007-07-01

Due to the recent incorporation of positron technology (PET) it appears interesting to review the present indications of fluor-deoxyglucose (FDG) in neuropediatric pathology. The main indication is in the evaluation of refractory epilepsy mainly in cases with negative magnetic resonance or discordance with electro encephalography Some uses in tumors are also discussed, such as tumor characterization and differentiation between radio necrosis and recurrence. The experience between years 2003 and 2007 at the Santiago Military Hospital, using FDG in 66 children and adolescents cases with diverse neurological indications was reviewed (au)

Usefulness of PET-FDG in pathology neuropediatric

International Nuclear Information System (INIS)

Massardo V, Teresa; Canessa G, Jose; Sierralta C, M.Paulina; Gonzalez E, Patricio; Jofre M, M.Josefina

2007-01-01

Due to the recent incorporation of positron technology (PET) it appears interesting to review the present indications of fluor-deoxyglucose (FDG) in neuropediatric pathology. The main indication is in the evaluation of refractory epilepsy mainly in cases with negative magnetic resonance or discordance with electro encephalography Some uses in tumors are also discussed, such as tumor characterization and differentiation between radio necrosis and recurrence. The experience between years 2003 and 2007 at the Santiago Military Hospital, using FDG in 66 children and adolescents cases with diverse neurological indications was reviewed (au)

Verification report for SIMREP 1.1

International Nuclear Information System (INIS)

Tarapore, P.S.

1987-06-01

SIMREP 1.1 is a discrete event computer simulation of repository operations in the surface waste-handling facility. The logic for this model is provided by Fluor Technology, Inc., the Architect/Engineer of the salt repository. The verification methods included a line-by-line review of the code, a detailed examination of a generated trace of all simulated events over a given period of operations, and a comparison of the simulation output results with expected values. SIMREP 1.1 performs in the required manner under the given range of input conditions

PPO-ethanol system as wavelength shifter for the Cherenkov counting technique using a liquid scintillation counter

International Nuclear Information System (INIS)

Takiue, M.; Fujii, H.; Ishikawa, H.

1984-01-01

2,5-diphenyloxazole (PPO) has been proposed as a wavelength shifter for Cherenkov counting. Since PPO is not incorporated with water, we have introduced the fluor into water in the form of micelle using a PPO-ethanol system. This technique makes it possible to obtain a high Cherenkov counting efficiency under stable sample conditions, attributed to the proper spectrometric features of the PPO. The 32 P Cherenkov counting efficiency (68.4%) obtained from this technique is 1.62 times as large as that measured with a conventional Cherenkov technique. (orig.)

Immediately lower emissions and often also lower energy demand. Exchange of refrigerants with only reduced technical expenditure; Sofort weniger Emissionen und oft auch weniger Energiebedarf. Kaeltemittelaustausch mit nur wenigen technischem Aufwand

Energy Technology Data Exchange (ETDEWEB)

Corr, Stuart; Cooper, Martyn [Mexichem Fluor, Runcorn (United Kingdom); Schwennesen, Karsten [Mexichem Fluor, Frankfurt am Main (Germany)

2012-11-15

The refrigeration industry as well as the air conditioning industry face again the challenges in the selection of refrigerants. The keyword here is Global Warming Potential (GWP). The search for the best solution for the future is already in full swing. But, can you do something already today? Yes, you can. A simple exchange of refrigerants with an enhanced GWP value such as R404A against a refrigerant with a lower GWP value such as KLEA407A from Mexichem Fluor can reduce the environmental impact immediately.

Measurement of detector neutron energy response using time-of-flight techniques

International Nuclear Information System (INIS)

Janee, H.S.

1973-09-01

The feasibility of using time-of-flight techniques at the EG and G/AEC linear accelerator for measuring the neutron response of relatively sensitive detectors over the energy range 0.5 to 14 MeV has been demonstrated. The measurement technique is described in detail as are the results of neutron spectrum measurements from beryllium and uranium photoneutron targets. The sensitivity of a fluor photomultiplier LASL detector with a 2- by 1-inch NE-111 scintillator was determined with the two targets, and agreement in the region of overlap was very good. (U.S.)

Detailed design data package: 3.1a-Film cooler pressure drop data; Item 3.2a - SBS packing selection; Item 3.2b, 3.2c - Pressure drop data for SBS distribution plate; and Item 3.2e - SBS distribution plate and liquid risers. PHTD pilot-scale melter testing system cost account milesonte 1.2.2.04.15A

International Nuclear Information System (INIS)

Whyatt, G.A.; Anderson, L.D.; Evans, J. II.

1996-03-01

This data package transmits information collected on the Liquid-Fed Ceramic Melter (LFCM) offgas system prior to melter feeding operations. Injection of steam to the melter plenum was used to simulate feeding of the melter. Steam surge cases were studied under steady-state surge conditions. Dynamic surges will be examined under data needs. The Fluor data needs included two blank tables requesting specific information for data needs 3.1 and 3.2. These tables are provided in Tables S.1 and S.2 below with the requested information filled in

Rapid quantitative assay for chloramphenicol acetyltransferase

International Nuclear Information System (INIS)

Neumann, J.R.; Morency, C.A.; Russian, K.O.

1987-01-01

Measuring the expression of exogenous genetic material in mammalian cells is commonly done by fusing the DNA of interest to a gene encoding an easily-detected enzyme. Chloramphenicol acetyltransferase(CAT) is a convenient marker because it is not normally found in eukaryotes. CAT activity has usually been detected using a thin-layer chromatographic separation followed by autoradiography. An organic solvent extraction-based method for CAT detection has also been described, as well as a procedure utilizing HPLC analysis. Building on the extraction technique, they developed a rapid sensitive kinetic method for measuring CAT activity in cell homogenates. The method exploits the differential organic solubility of the substrate ([ 3 H] or [ 14 C]acetyl CoA) and the product (labeled acetylchloramphenicol). The assay is a simple one-vial, two-phase procedure and requires no tedious manipulations after the initial setup. Briefly, a 0.25 ml reaction with 100mM Tris-HCL, 1mM chloramphenicol, 0.1mM [ 14 C]acetyl CoA and variable amounts of cell homogenate is pipetted into a miniscintillation vial, overlaid with 5 ml of a water-immiscible fluor, and incubated at 37 0 C. At suitable intervals the vial is counted and the CAT level is quantitatively determined as the rate of increase in counts/min of the labeled product as it diffuses into the fluor phase, compared to a standard curve. When used to measure CAT in transfected Balb 3T3 cells the method correlated well with the other techniques

Preparation, installation, and calibration of a 152-fiber imaging experiment at the Nevada Test Site

International Nuclear Information System (INIS)

Thayer, D.R.; Lyons, P.B.; Looney, L.D.; Manning, J.P.; Malone, R.M.

1981-01-01

Fiber-optic transmission lines are being used with increasing frequency in the demanding environment of nuclear device diagnostic tests at the Nevada Test Site. Previous reports have described diagnostic experiments that utilize properties of fiber-optic cables to provide capabilities that are extremely difficult to obtain with coaxial cable systems. This paper describes an imaging experiment conducted during the last quarter of 1980 at the Nevada Test Site involving 152 approx. 0.6-km long GI fibers in 18 cables. An imager cell sensitive to neutron and gamma radiation was located at approx. 8 m from the source. Individual elements of the fluor were coupled by approx. 10 m PCS fibers (for radiation damage resistance) to the GI fibers used for the uphole and surface lines to the detector station. Light from the imager cell in a 9-nm band, centered at 540 nm, was detected by photomultipliers and the electrical signals then recorded on oscilloscopes. Overall system bandwidth, including the fluor, was approx. 80 MHz. Due to the complexity of the experiment and the conditions associated with field testing, the PCS and GI fibers were cut and terminated under laboratory shop conditions prior to installation at the field site. Procedures used for quality assurance of the fiber assemblies as well as procedures used in checking fiber throughput during installation are described. The application of a large star coupler (5 input x 200 output fibers) used for system time and amplitude calibration and an attempt to shutter fibers with neutrons for a multiplexing application are also discussed

Improving Efficiency with 3-D Imaging: Technology Essential in Removing Plutonium Processing Equipment from Plutonium Finishing Plant Gloveboxes

International Nuclear Information System (INIS)

Crow, Stephen H.; Kyle, Richard N.; Minette, Michael J.

2008-01-01

The Plutonium Finishing Plant at Hanford, Washington began operations in 1949 to process plutonium and plutonium products. Its primary mission was to produce plutonium metal, fabricate weapons parts, and stabilize reactive materials. These operations, and subsequent activities, were performed in remote production lines, consisting primarily of hundreds of gloveboxes. Over the years these gloveboxes and processes have been continuously modified. The plant is currently inactive and Fluor Hanford has been tasked to clean out contaminated equipment and gloveboxes from the facility so it can be demolished in the near future. Approximately 100 gloveboxes at PFP have been cleaned out in the past four years and about 90 gloveboxes remain to be cleaned out. Because specific commitment dates for this work have been established with the State of Washington and other entities, it is important to adopt work practices that increase the safety and speed of this effort. The most recent work practice to be adopted by Fluor Hanford D and D workers is the use of 3-D models to improve the efficiency of cleaning out radioactive gloveboxes at the plant. The use of 3-D models has significantly improved the work planning process by providing workers with a clear image of glovebox construction and composition, which is then used to determine cleanout methods and work sequences. The 3-D visual products enhance safety by enabling workers to more easily identify hazards and implement controls. In addition, the ability to identify and target the removal of radiological materials early in the D and D process provides substantial dose reduction for the workers

AIR PERMIT COMPLIANCE FOR WASTE RETRIEVAL OEPRATIONS INVOLVING MULTI-UNIT EMISSIONS

International Nuclear Information System (INIS)

SIMMONS FM

2007-01-01

Since 1970, approximately 38,000 suspect-transuranic and transuranic waste containers have been placed in retrievable storage on the Hanford Site in the 200 Areas burial grounds. Hanford's Waste Retrieval Project is retrieving these buried containers and processing them for safe storage and disposition. Container retrieval activities require an air emissions permit to account for potential emissions of radionuclides. The air permit covers the excavation activities as well as activities associated with assaying containers and installing filters in the retrieved transuranic containers lacking proper venting devices. Fluor Hanford, Inc. is required to track radioactive emissions resulting from the retrieval activities. Air, soil, and debris media contribute to the emissions and enabling assumptions allow for calculation of emissions. Each of these activities is limited to an allowed annual emission (per calendar year) and .contributes to the overall total emissions allowed for waste retrieval operations. Tracking these emissions is required to ensure a permit exceedance does not occur. A tracking tool was developed to calculate potential emissions in real time sense. Logic evaluations are established within the tracking system to compare real time data against license limits to ensure values are not exceeded for either an individual activity or the total limit. Data input are based on field survey and workplace air monitoring activities. This tracking tool is used monthly and quarterly to verify compliance to the license limits. Use of this tool has allowed Fluor Hanford, Inc. to successfully retrieve a significant number of containers in a safe manner without any exceedance of emission limits

Fracture load of different crown systems on zirconia implant abutments.

Science.gov (United States)

Albrecht, T; Kirsten, A; Kappert, H F; Fischer, H

2011-03-01

The purpose of this study was to evaluate the fracture load of single zirconia abutment restorations using different veneering techniques and materials. The abutment restorations were divided into 6 groups with 20 samples each: test abutments (control group A), lithium disilicate ceramic crowns bonded on incisor abutments (group B), leucite ceramic crowns bonded on incisor abutments (group C), premolar abutments directly veneered with a fluor apatite ceramic (group D (layered) and group E (pressed)) and premolar abutments bonded with lithium disilicate ceramic crowns (group F). The fracture load of the restorations was evaluated using a universal testing machine. Half of each group was artificially aged (chewing simulation and thermocycling) before evaluating the fracture load with the exception of the test abutments. The fracture load of the test abutments was 705 ± 43N. Incisor abutments bonded with lithium disilicate or leucite ceramic crowns (groups B and C) showed fracture loads of about 580N. Premolar restorations directly veneered with fluor apatite ceramic (groups D and E) showed fracture loads of about 850N. Premolar restorations bonded with lithium disilicate ceramic crowns (group F) showed fracture loads of about 1850N. The artificial ageing showed no significant influence on the strength of the examined restorations. All ceramic crowns made of lithium disilicate glass-ceramic, adhesively bonded to premolar abutments showed the highest fracture loads in this study. However, all tested groups can withstand physiological bite forces. Copyright © 2010 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Toward photostable multiplex analyte detection on a single mode planar optical waveguide

Energy Technology Data Exchange (ETDEWEB)

Mukundan, Harshini [Los Alamos National Laboratory; Xei, Hongshi [Los Alamos National Laboratory; Anderson, Aaron S [Los Alamos National Laboratory; Grace, Wynne K [Los Alamos National Laboratory; Martinez, Jennifer S [NON LANL; Swanson, Basil [Los Alamos National Laboratory

2009-01-01

We have developed a waveguide-based optical biosensor for the sensitive and specific detection of biomarkers associated with disease. Our technology combines the superior optical properties of single-mode planar waveguides, the robust nature of functionalized self-assembled monolayer sensing films and the specificity of fluorescence sandwich immunoassays to detect biomarkers in complex biological samples such as serum, urine and sputum. We have previously reported the adaptation of our technology to the detection of biomarkers associated with breast cancer and anthrax. However, these approaches primarily used phospholipid bilayers as the functional film and organic dyes (ex: AlexaFluors) as the fluorescence reporter. Organic dyes are easily photodegraded and are not amenable to multiplexing because of their narrow Stokes' shift. Here we have developed strategies for conjugation of the detector antibodies with quantum dots for use in a multiplex detection platform. We have previously evaluated dihydroxylipoic acid quantum dots for the detection of a breast cancer biomarker. In this manuscript, we investigate the detection of the Bacillus anthracis protective antigen using antibodies conjugated with polymer-coated quantum dots. Kinetics of binding on the waveguide-based biosensor is reported. We compare the sensitivity of quantum dot labeled antibodies to those labeled with AlexaFluor and demonstrate the photostability of the former in our assay platform. In addition, we compare sulfydryl labeling of the antibody in the hinge region to that of nonspecific amine labeling. This is but the first step in developing a multiplex assay for such biomarkers on our waveguide platform.

A fluorescence resonance energy transfer-based approach for investigating late endosome–lysosome retrograde fusion events

Science.gov (United States)

Kaufmann, A.M.; Goldman, S.D.B.; Krise, J.P.

2009-01-01

Traditionally, lysosomes have been considered to be a terminal endocytic compartment. Recent studies suggest that lysosomes are quite dynamic, being able to fuse with other late endocytic compartments as well as with the plasma membrane. Here we describe a quantitative fluorescence energy transfer (FRET)-based method for assessing rates of retrograde fusion between terminal lysosomes and late endosomes in living cells. Late endosomes were specifically labeled with 800-nm latex beads that were conjugated with streptavidin and Alexa Fluor 555 (FRET donor). Terminal lysosomes were specifically labeled with 10,000-MW dextran polymers conjugated with biotin and Alexa Fluor 647 (FRET acceptor). Following late endosome–lysosome fusion, the strong binding affinity between streptavidin and biotin brought the donor and acceptor fluorophore molecules into close proximity, thereby facilitating the appearance of a FRET emission signal. Because apparent size restrictions in the endocytic pathway do not permit endocytosed latex beads from reaching terminal lysosomes in an anterograde fashion, the appearance of the FRET signal is consistent with retrograde transport of lysosomal cargo back to late endosomes. We assessed the efficiency of this transport step in fibroblasts affected by different lysosome storage disorders—Niemann–Pick type C, mucolipidosis type IV, and Sandhoff’s disease, all of which have a similar lysosomal lipid accumulation phenotype. We report here, for the first time, that these disorders can be distinguished by their rate of transfer of lysosome cargos to late endosomes, and we discuss the implications of these findings for developing new therapeutic strategies. PMID:19109922

Modelling a Global EPCM (Engineering, Procurement and Construction Management Enterprise

Directory of Open Access Journals (Sweden)

Sekhar Chattopadhyay

2010-03-01

Full Text Available This paper investigates the applicability of enterprise architectures in the context of current business environment by examining the application of Purdue Enterprise Reference Architecture to WorleyParsons, a global engineering, procurement and construction management enterprise, under the backdrop of a similar study carried out on Fluor Daniel during mid-nineties of the last century. The outcome of this study recommends the need for new enterprise architecture, the People-Centric Enterprise Architecture that not only focuses on human dimension in modern enterprises as the central thread, but also includes more business characteristics of the enterprise other than engineerings.

Fluorine toxicity in corn plants nearby a ceramic industry, Araras, State of São Paulo, Brazil

OpenAIRE

Fortes, Caio; Duarte, Aildson Pereira; Matsuoka, Sizuo; Hoffman, Hermann P.; Lavorenti, Norberto Antônio

2003-01-01

Indústrias que submetem material terroso a altas temperaturas como a de cerâmicas, emitem fluoretos para a atmosfera na forma de gás, particulado ou ácido fluorídrico. Em experimentos de milho (Zea mays L.), do Centro de Ciências Agrárias da Universidade Federal de São Carlos, Araras (SP), as plantas apresentaram sintomas que evoluíam de uma descoloração do limbo foliar até o secamento marginal das folhas. O objetivo deste trabalho foi avaliar os teores foliares de flúor (F) na cultura do mil...

Triton X-100 as a complete liquid scintillation cocktail for counting aqueous solutions and ionic nutrient salts

International Nuclear Information System (INIS)

Reed, D.W.

1984-01-01

Triton X-100, used alone, was found to act as a complete liquid scintillation cocktail. Triton X-100 acted as a scintillator and the effect was not due to Cerenkov radiation. A variety of other commercially available surfactants also acted as scintillators, but with different levels of efficiency. Triton X-100/water combinations were suitable for counting aqueous solutions of 33 P and 86 Rb and the count rate was stable over extended periods of time. Triton X-100/toluene combinations also yielded high counting efficiencies. Triton X-100 was more sensitive to quenching than standard cocktails containing fluors. (author)

TRANSITION & CLOSEOUT OF THE FERNALD CLOSURE PROJECT

Energy Technology Data Exchange (ETDEWEB)

BILSON, H.E.

2007-02-01

The US Department of Energy (DOE) and Fluor Fernald have completed the majority of the cleanup of the Fernald Site. The over 1000 acre complex for processing uranium has been demolished and soil contamination has been remediated. With acres of wetlands and prairies replacing the buildings and waste pits. At the end of the project the focus shifted to developing demonstrating the completion of the project and the contract, as well as ensuring a smooth transition of the facility from the DOE's Environmental Management (EM) Program to the DOE's Legacy Management (LM) Program.

NHC's contribution to cleanup of the Hanford Site

International Nuclear Information System (INIS)

Chauve, H.D.

1998-01-01

The one billion dollars per year Project Hanford Management Contract (PHMC), managed by Fluor Daniel Hanford, calls for cleanup of the Hanford Site for the Department of Energy. Project Hanford comprises four major subprojects, each managed by a different major contractor. Numatec Hanford Corporation (NHC) is a fifth major subcontractor which provides energy and technology to each of the Hanford projects. NHC draws on the experience and capabilities of its parent companies, COGEMA and SGN, and relies on local support from its sister Company in Richland, COGEMA Engineering Corporation, to bring the best commercial practices and new technology to the Project

Cu2+-labeled dansyl compounds as fluorescent and PET probes for imaging apoptosis.

Science.gov (United States)

Han, Junyan; Wang, Xukui; Yu, MeiXiang

2016-11-15

Compound DNSTT-Cu 2+ , a novel chelate of Cu 2+ with DOTA conjugated to a fluorescent dansyl fragment, is developed for imaging cell apoptosis. Apoptotic U-87MG cells could be selectively visualized by the fluorescence of DNSTT-Cu 2+ from cytoplasm of cells, confirmed by the fluorescence of apoptosis cells co-labeled with Alexa Fluor 568-labeled annexin V, a conventional probe for selectively labeling membranes of apoptosis cells. A radioactive 64 Cu 2 + analog, DNSTT- 64 Cu 2+ , was easily synthesized, providing a potential PET probe for imaging apoptosis in vivo. Copyright © 2016 Elsevier Ltd. All rights reserved.

Obtenção e caracterização de membranas assimétricas via sol-gel

OpenAIRE

Lenza,R. F. S.; Costa,R. O. R.; Vasconcelos,W. L.

2002-01-01

Foram obtidas membranas de sílica via sol-gel, utilizando-se tetraetilortossilicato (TEOS) como precursor em meio ácido. A estrutura da membrana é composta de uma camada de sílica mesoporosa (raio médio de poros igual a 21,6 nm) recoberta por um filme microporoso de sílica (raio médio de poros igual a 1,1 nm). A camada suporte foi obtida utilizando-se ácido fluorídrico como catalisador, enquanto que para a fabricação do filme ativo foi utilizado ácido nítrico. A camada suporte apresenta área ...

Training survey -- educational profile for Hanford HANDI 2000 project

Energy Technology Data Exchange (ETDEWEB)

Wilson, D.

1998-08-25

Fluor Daniel Hanford, Inc. (FDH) is currently adopting streamlined business processes through integrated software solutions. Replacing the legacy software (current/replacement systems, attached) also avoids significant maintenance required to resolve Year 2000 issues. This initiative is being referred to as `HANDI 2000`. The software being implemented in the first phase of this project includes Indus International`s PASSPORT Software, Peoplesoft and Primavera P3 Software. The project, which encompasses all the system replacements that will occur, has been named `HANDI 2000.` The PASSPORT applications being implemented are Inventory Management, Purchasing, Contract Management, Accounts Payable, and MSDS (Material Safety Data Sheets).

El efecto del entorno en la emisión de fluorescencia . Modelos teóricos y aplicaciones analíticas

OpenAIRE

Delgado Camón, Mª Arantzazu

2012-01-01

En esta Tesis Doctoral se abordan aspectos teóricos y prácticos de la fluorescencia molecular, en medio líquido y sólido, que pueden resumirse en: 1.- Estudio y utilización del efecto del entorno molecular en la intensidad de fluorescencia, que se refiere a cómo las interacciones no covalentes que sufre un fluoróforo en presencia de un analito y un disolvente afectan a su emisión y a la utilización de esos cambios para obtener información analítica del entorno. Para ello se ha desarrollad...

Estudio del grado de madurez y/o maduración de tomates empleando técnicas espectroscópicas moleculares

OpenAIRE

Jurado Capel, Laura

2012-01-01

Este Trabajo Fin de Máster versa sobre el estudio de la madurez en tomates evaluando la evolución de distintos pigmentos (carotenos, tocoferoles, flavonoides)y otros compuestos como el glutamato monosódico. Para ello se hace uso de técnicas espectroscópicas moleculares donde el análisis de la muestra se aborda a través de dos estrategias diferentes que permita comparar el análisis directo sobre la piel del fruto mediante ATR-FTIR y análisis de extractos metanólicos de la piel mediante Fluore...

Rapid separation and determination of 107Pd in radioactive waste produced during NPP A-1 decommissioning

International Nuclear Information System (INIS)

Boris Andris; Miroslav Prazsky; Ferdinand Sebesta

2015-01-01

Procedure for 107 Pd determination in radioactive wastes coming from decommissioning of NPP A-1 is proposed and verified. 107 Pd was separated and purified by Pd precipitation with dimethylglyoxime (DMG) and/or ferric hydroxide precipitation. 107 Pd yield was determined gravimetrically by weighing the Pd(DMG) 2 precipitate. 107 Pd counting was performed using liquid scintillation counter (LSC-TDCR) and scintillation cocktail Hionic Fluor. In all analyzed samples the determined activities of 107 Pd were lower than MDA. Nevertheless such analyses permit the decision concerning the storage of solidified wastes in the Mochovce regional repository. (author)

Pacific Northwest National Laboratory Apatite Investigation at the 100-NR-2 Quality Assurance Project Plan

Energy Technology Data Exchange (ETDEWEB)

Fix, N. J.

2008-03-28

This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the 100-NR-2 Apatite Project. The U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N would include apatite sequestration as the primary treatment, followed by a secondary treatment. The scope of this project covers the technical support needed before, during, and after treatment of the targeted subsurface environment using a new high-concentration formulation.

Solid Waste Integrated Forecast Technical (SWIFT) Report FY 2001 to FY 2046 Volume 1

International Nuclear Information System (INIS)

BARCOT, R.A.

2001-01-01

In an effort to maintain an up-to-date Solid Waste Forecast, Fluor Hanford, Inc. requested a midyear update to the FY2001 Solid Waste Forecast collected in June 2000. Below is a list of generators reporting significant changes from the baseline FY2001 forecast, as well as approved generators previously not reporting wastes. The updated data was collected in February 2001. The cumulative effect of the changes in the near term was minor. Included in this update are waste generation maps for onsite facilities. The maps are updated annually and use the baseline forecast as a data reference

ATR Spent Fuel Options Study

International Nuclear Information System (INIS)

Connolly, Michael James; Bean, Thomas E.; Brower, Jeffrey O.; Luke, Dale E.; Patterson, M. W.; Robb, Alan K.; Sindelar, Robert; Smith, Rebecca E.; Tonc, Vincent F.; Tripp, Julia L.; Winston, Philip L.

2017-01-01

The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center's (INTEC's) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

ATR Spent Fuel Options Study

Energy Technology Data Exchange (ETDEWEB)

Connolly, Michael James [Idaho National Lab. (INL), Idaho Falls, ID (United States); Bean, Thomas E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Brower, Jeffrey O. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Luke, Dale E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Patterson, M. W. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Robb, Alan K. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Sindelar, Robert [Idaho National Lab. (INL), Idaho Falls, ID (United States); Smith, Rebecca E. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tonc, Vincent F. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Tripp, Julia L. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Winston, Philip L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2017-01-01

The Advanced Test Reactor (ATR) is a materials and fuels test nuclear reactor that performs irradiation services for the U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), Naval Reactors, the National Nuclear Security Administration (NNSA), and other research programs. ATR achieved initial criticality in 1967 and is expected to operate in support of needed missions until the year 2050 or beyond. It is anticipated that ATR will generate approximately 105 spent nuclear fuel (SNF) elements per year through the year 2050. Idaho National Laboratory (INL) currently stores 2,008 ATR SNF elements in dry storage, 976 in wet storage, and expects to have 1,000 elements in wet storage before January 2017. A capability gap exists at INL for long-term (greater than the year 2050) management, in compliance with the Idaho Settlement Agreement (ISA), of ATR SNF until a monitored retrievable geological repository is open. INL has significant wet and dry storage capabilities that are owned by the DOE Office of Environmental Management (EM) and operated and managed by Fluor Idaho, which include the Idaho Nuclear Technology and Engineering Center’s (INTEC’s) CPP-666, CPP-749, and CPP-603. In addition, INL has other capabilities owned by DOE-NE and operated and managed by Battelle Energy Alliance, LLC (BEA), which are located at the Materials and Fuel Complex (MFC). Additional storage capabilities are located on the INL Site at the Naval Reactors Facility (NRF). Current INL SNF management planning, as defined in the Fluor Idaho contract, shows INTEC dry fuel storage, which is currently used for ATR SNF, will be nearly full after transfer of an additional 1,000 ATR SNF from wet storage. DOE-NE tasked BEA with identifying and analyzing options that have the potential to fulfill this capability gap. BEA assembled a team comprised of SNF management experts from Fluor Idaho, Savannah River Site (SRS), INL/BEA, and the MITRE Corp with an objective of developing and analyzing

The influences of sensitivity of ligand in determination of fluors with LaF3

International Nuclear Information System (INIS)

Muzakky; Lahagu, F.; Djawahiri, H.M.; Susiaturi, E.

1996-01-01

The influences of acetate and carbonate ligand as a binder of uranium in uranium-tetra fluoride solution, with 0,1 M HNO 3 acid condition was been studied. The aim of binding is to looses of uranium from UO 2 (F 4 ) 2- compound, so the free of fluoride were able detected with LaF 3 membrane electrode on potentiometric method. Base on the free of Fluoride, acetate ligand was more sensitive than carbonate ligand. The addition method was able to prevented influent of HF species in high acidity was realized. The method was use to determination of Fluoride in C oncentrated Uranium , and yield are 1,215x10 -5 M±1,957x10 -4 in acetate ligand and 3,957x10 -5 M±2,57x10 -4 in carbonate ligand. (author)

PENGARUH SUMBER MINERAL TERHADAP PENEKANAN Erwinia carotovora OLEH PSEUDOMONAS PENDAR-FLUOR SECARA IN VITRO

Directory of Open Access Journals (Sweden)

Hardian Susilo Addy .

2012-02-01

Full Text Available Antimicrobial Stimulation of Fluorescent Pseudomonad to Inhibit Soft-rot Pathogen Caused by Erwinia carotovora subsp. Carotovora. This research was conducted to study effect of mineral sources on inhibition Erwinia carotovora by fluorescent pseudomonad. We used several mineral sources to stimulate antimicrobial substances from fluorescent pseudomonad that responsible to inhibit E. carotovora subsp. carotovora in vitro. The results showed that zinc 0,5 mM were the best to increase antagonistics of fluorescent psudomonad againts E. carotovora. Zinc were increased antimicrobial substances twohold compared with control without stimulant agent. Detection of antimicrobial substance using TLC showed that only one antimicrobial was detected with retention factor (Rf of 0,68 – 0,72. However, identification and characterization of that substance is still needed.

1998 interim 242-A Evaporator tank system integrity assessment report

Energy Technology Data Exchange (ETDEWEB)

Jensen, C.E.

1998-07-02

This Integrity Assessment Report (IAR) is prepared by Fluor Daniel Northwest (FDNW) under contract to Lockheed-Martin Hanford Company (LMHC) for Waste Management Hanford (WMH), the 242-A Evaporator (facility) operations contractor for Fluor Daniel Hanford, and the US Department of Energy, the system owner. The contract specifies that FDNW perform an interim (5 year) integrity assessment of the facility and prepare a written IAR in accordance with Washington Administrative Code (WAC) 173-303-640. The WAC 173-303 defines a treatment, storage, or disposal (TSD) facility tank system as the ``dangerous waste storage or treatment tank and its ancillary equipment and containment.`` This integrity assessment evaluates the two tank systems at the facility: the evaporator vessel, C-A-1 (also called the vapor-liquid separator), and the condensate collection tank, TK-C-100. This IAR evaluates the 242-A facility tank systems up to, but not including, the last valve or flanged connection inside the facility perimeter. The initial integrity assessment performed on the facility evaluated certain subsystems not directly in contact with dangerous waste, such as the steam condensate and used raw water subsystems, to provide technical information. These subsystems were not evaluated in this IAR. The last major upgrade to the facility was project B-534. The facility modifications, as a result of project B-534, were evaluated in the 1993 facility interim integrity assessment. Since that time, the following upgrades have occurred in the facility: installation of a process condensate recycle system, and installation of a package steam boiler to provide steam for the facility. The package boiler is not within the scope of the facility TSD.

Description of dental caries status of children aged 12 – 15 years in Berambai village, North Samarinda, East Kalimantan

Directory of Open Access Journals (Sweden)

Nurul Rahmadhani Nur

2016-06-01

Full Text Available Based on data RISKESDAS 2007 and 2013, national prevalence of oral and dental problems increased by 2.4% and national prevalence of active caries increased by 9.8%. National DMF-T index value is 4.6 in 2013. East Kalimantan has an active caries prevalence and DMF-T index value higher than the national average. Berambai village is one of the villages in the North Samarinda, East Kalimantan whose residents have problems with public water supply. The residents use ground water as their main source of clean water. The purpose of this study is to evaluate the caries status and fluoride content of drinking water consumed by Berambai children aged 12 – 15 years. This study is an observational study with cross sectional descriptive design, which is conducted in Berambai village, North Samarinda, East Kalimantan and Health Laboratory of East Kalimantan. The study was carried out in November 2015 involving children aged 12 – 15 years, born in that village until the study was performed. In addition, the fluoride content of drinking water was also evaluated. Each participant filled out the questionnaire and was then examinated his dental caries using DMF-T index. Data was analyzed by univariate analysis. The results showed that the average value of DMF-T was 3.92 (medium category, while the boys had DMF-T 4.63 (high category and the girls had DMF-T 3.14 (medium category. The fluor levels of drinking water is 0.00 ppm (low category. In conclusion, the caries status of children aged 12 – 15 years was in medium category and the fluor levels of drinking water was very low.

Influence of air pollution on cultivated plants

Energy Technology Data Exchange (ETDEWEB)

Spierings, F H.F.G.; Van Raay, A

1962-01-01

Fumigation experiments were carried out with tulips, gladioli and freesia to investigate the correlation between the extent of leaf injury caused by hydrogen fluoride, the fluor content of the damaged leaves and the fluor content of the air in the fumigation chambers. Fumigation experiments with SO/sub 2/ were carried out on several vegetables, e.g. endive, spinach, celery and tomato. On 28 experimental plots distributed throughout a horticultural area, the symptoms of HF damage were studied on freesia plants. At a distance of 6 km from the industrial area, 20% of the length of the leaves appeared to be damaged. 10 km from this area the percentage of damage was 15 and on the most distant plots an injury of 5% was still present. On more than 20 places in The Netherlands investigations have been carried out about injury of plants in the neighborhood of factories. In the vicinity of a cement-industry, an investigation has been started about the extent and the composition of dust from this industry deposited on vegetables and fruits. The monthly quantity of rain water was determined and the solid deposits in the rain were quantitatively and qualitatively investigated under the microscope. In the area near a hydrocyanic acid factory, damage was done to grass and to fruit trees. It was demonstrated by leaf analyses in both cases that the damage was caused by SO/sub 2/. Other cases of crop damage were imputed to poisoning of the air by sulfur dioxide or hydrogen fluoride, to acid containing soot deposits, to zinc oxide vapors and phenol containing vapors of resins.

Wide-field lifetime-based FRET imaging for the assessment of early functional distribution of transferrin-based delivery in breast tumor-bearing small animals

Science.gov (United States)

Sinsuebphon, Nattawut; Rudkouskaya, Alena; Barroso, Margarida; Intes, Xavier

2016-02-01

Targeted drug delivery is a critical aspect of successful cancer therapy. Assessment of dynamic distribution of the drug provides relative concentration and bioavailability at the target tissue. The most common approach of the assessment is intensity-based imaging, which only provides information about anatomical distribution. Observation of biomolecular interactions can be performed using Förster resonance energy transfer (FRET). Thus, FRET-based imaging can assess functional distribution and provide potential therapeutic outcomes. In this study, we used wide-field lifetime-based FRET imaging for the study of early functional distribution of transferrin delivery in breast cancer tumor models in small animals. Transferrin is a carrier for cancer drug delivery. Its interaction with its receptor is within a few nanometers, which is suitable for FRET. Alexa Fluor® 700 and Alexa Fluor® 750 were conjugated to holo-transferrin which were then administered via tail vein injection to the mice implanted with T47D breast cancer xenografts. Images were continuously acquired for 60 minutes post-injection. The results showed that transferrin was primarily distributed to the liver, the urinary bladder, and the tumor. The cellular uptake of transferrin, which was indicated by the level of FRET, was high in the liver but very low in the urinary bladder. The results also suggested that the fluorescence intensity and FRET signals were independent. The liver showed increasing intensity and increasing FRET during the observation period, while the urinary bladder showed increasing intensity but minimal FRET. Tumors gave varied results corresponding to their FRET progression. These results were relevant to the biomolecular events that occurred in the animals.

1998 interim 242-A Evaporator tank system integrity assessment report

International Nuclear Information System (INIS)

Jensen, C.E.

1998-01-01

This Integrity Assessment Report (IAR) is prepared by Fluor Daniel Northwest (FDNW) under contract to Lockheed-Martin Hanford Company (LMHC) for Waste Management Hanford (WMH), the 242-A Evaporator (facility) operations contractor for Fluor Daniel Hanford, and the US Department of Energy, the system owner. The contract specifies that FDNW perform an interim (5 year) integrity assessment of the facility and prepare a written IAR in accordance with Washington Administrative Code (WAC) 173-303-640. The WAC 173-303 defines a treatment, storage, or disposal (TSD) facility tank system as the ''dangerous waste storage or treatment tank and its ancillary equipment and containment.'' This integrity assessment evaluates the two tank systems at the facility: the evaporator vessel, C-A-1 (also called the vapor-liquid separator), and the condensate collection tank, TK-C-100. This IAR evaluates the 242-A facility tank systems up to, but not including, the last valve or flanged connection inside the facility perimeter. The initial integrity assessment performed on the facility evaluated certain subsystems not directly in contact with dangerous waste, such as the steam condensate and used raw water subsystems, to provide technical information. These subsystems were not evaluated in this IAR. The last major upgrade to the facility was project B-534. The facility modifications, as a result of project B-534, were evaluated in the 1993 facility interim integrity assessment. Since that time, the following upgrades have occurred in the facility: installation of a process condensate recycle system, and installation of a package steam boiler to provide steam for the facility. The package boiler is not within the scope of the facility TSD

CY2003 RCRA GROUNDWATER MONITORING WELL SUMMARY REPORT

International Nuclear Information System (INIS)

MARTINEZ, C.R.

2003-01-01

This report describes the calendar year (CY) 2003 field activities associated with the installation of two new groundwater monitoring wells in the A-AX Waste Management Area (WMA) and four groundwater monitoring wells in WMA C in the 200 East Area of the Hanford Nuclear Reservation. All six wells were installed by Fluor Hanford Inc. (FH) for CH2M Hill Hanford Group, Inc. (CHG) in support of Draft Hanford Facility Agreement and Consent Order (Tri-Party Agreement) M-24-00 milestones and ''Resource Conservation and Recovery Act of 1976'' (RCRA) groundwater monitoring requirements. Drilling data for the six wells are summarized in Table 1

Influencia del CaF2 sobre la capacidad de reacción de crudos de cemento

OpenAIRE

Palomo, Ángel; Blanco, M. Teresa

1982-01-01

En este trabajo se estudia el efecto de una pequeña cantidad de CaF2 sobre la capacidad de reacción de crudos de cemento, para lo cual se comparan los comportamientos térmicos en función del tiempo de clinkerización de un crudo comercial con y sin adición de CaF2. Se da una estimación de los tiempos necesarios para completar las reacciones a diferentes temperaturas. El efecto mineralizador del espato-fluor pasa por un máximo a temperaturas de clinkerización del orden de 1.200ºC y tiempos d...

Training Workers to use Localized Ventilation for Radiological Work

International Nuclear Information System (INIS)

WAGGONER, L.O.

2000-01-01

Work on radiological systems and components needs to be accomplished using techniques that reduce radiation dose to workers, limit contamination spread, and minimize radioactive waste. One of the best methods to control contamination spread is to use localized ventilation to capture radioactive material and keep it from spreading. The Fluor Hanford ALARA Center teaches workers how to use ventilation in partnership with other engineered controls and this has resulted in improved work practices, minimized the impact on adjacent work operations, and decreased the amount of radioactive waste generated. This presentation will emphasize how the workers are trained to use localized ventilation for contamination control

Radiation damage in plastic scintillators

International Nuclear Information System (INIS)

Majewski, S.

1990-01-01

Results of radiation damage studies in plastic scintillators are reviewed and critically analyzed from the point of view of applications of plastic scintillators in calorimetric detectors for the SSC. Damage to transmission and to fluorescent yield in different conditions is discussed. New directions in R ampersand D are outlined. Several examples are given of the most recent data on the new scintillating materials made with old and new plastics and fluors, which are exhibiting significantly improved radiation resistance. With a present rate of a vigorous R D programme, the survival limits in the vicinity of 100 MRad seem to be feasible within a couple of years

La fluoración de aguas en la región de Murcia

OpenAIRE

Marín Camaches Maria Dolores; Pacheco Martínez Francisco; Martínez Gambín Remedios; Gomez Campoy Elisa; Gutierrez Molina Carolina

1998-01-01

FUNDAMENTO: El objeto de este trabajo es exponer las concentraciones naturales del ión fluor en las aguas de abastecimiento público de todos los municipios de la Región de Murcia en el año 1991, así como la evolución de dichas concentraciones en el periodo 1992-96 en el que se ha producido una fluoración en el suministro de agua como medida sanitaria pública. MÉTODOS: Los métodos utilizados para las determinaciones han sido el de la valoración potenciométrica mediante adición estándar y el de...

24 Command Fire Improvement Action Program Plan

International Nuclear Information System (INIS)

GRIFFIN, G.B.

2000-01-01

Fluor Hanford (FH) is responsible for providing support to the Department of Energy Richland Operations Office (RL) in the implementation of the Hanford Emergency Preparedness (EP) program. During fiscal year 2000, a number of program improvements were identified from various sources including a major range fire (24 Command Fire). Evaluations of the emergency preparedness program have confirmed that it currently meets all requirements and that performance of personnel involved is good, however the desire to effect continuous improvement resulted in the development of this improvement program plan. This program plan defines the activities that will be performed in order to achieve the desired performance improvements

Performance of 2000CA/LL and 2260XL liquid scintillation spectrometers for low-level tritium and carbon-14 analyses

International Nuclear Information System (INIS)

Rozanski, K.; Stichler, W.; Schwarz, P.

1991-01-01

The performance of two commercially available liquid scintillation spectrometers for low-level counting of 3 H and 14 C has been investigated. Two models of new technology Tri-Carb spectrometers from the Packard Instrument Company were tested: the 2000CA/LL and 2260XL. The measurements revealed a superior 3 H performance of the 2000CA/LL equipment owing to its substantially higher counting efficiency. Results for 14 C are less conclusive and depend on the configuration of the counting parameters selected. A comparison of three commercially available scintillation cocktails for tritium assay in water samples revealed a superior performance for PicoFluor LLT. (Author)

Assessment of Chemical and Radiological Vulnerabilities

International Nuclear Information System (INIS)

SETH, S.S.

2000-01-01

Following the May 14, 1997 chemical explosion at Hanford's Plutonium Reclamation Facility, the Department of Energy Richland Operations Office and its prime contractor, Fluor Hanford, Inc., completed an extensive assessment to identify and address chemical and radiological safety vulnerabilities at all facilities under the Project Hanford Management Contract. This was a challenging undertaking because of the immense size of the problem, unique technical issues, and competing priorities. This paper focuses on the assessment process, including the criteria and methodology for data collection, evaluation, and risk-based scoring. It does not provide details on the facility-specific results and corrective actions, but discusses the approach taken to address the identified vulnerabilities

PMT signal increase using a wavelength shifting paint

Energy Technology Data Exchange (ETDEWEB)

Allada, K. [Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Hurlbut, Ch. [Eljen Technology, Sweetwater, TX 79556 (United States); Ou, L.; Schmookler, B. [Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Shahinyan, A. [Yerevan Physics Institute, Yerevan (Armenia); Wojtsekhowski, B., E-mail: bogdanw@jlab.org [Thomas Jefferson National Accelerator Facility, Newport News, VA 23606 (United States)

2015-05-11

We report a 1.65 times increase of the PMT signal and a simple procedure of application of a new wavelength shifting (WLS) paint for PMTs with non-UV-transparent windows. Samples of four different WLS paints, made from hydrocarbon polymers and organic fluors, were tested on a 5-in. PMT (ET 9390KB) using Cherenkov radiation produced in fused silica disks by {sup 106}Ru electrons on a ‘table-top’ setup. The best performing paint was employed on two different types of 5-in. PMTs (ET 9390KB and XP4572B), installed in atmospheric pressure CO{sub 2} gas Cherenkov detectors, and tested using GeV electrons.

¿Agua de consumo humano con o sin flúor?

Directory of Open Access Journals (Sweden)

Jaime-Gonzáles-Murillo

2013-01-01

Full Text Available Los beneficios de la utilización de Flúor en la salud dental han sido convencionalmente aceptados, sinembargo en la actualidad nuevas investigaciones en salud humana revelan la urgencia por evitar la ingestión de Fluor al punto de inclusive modificar programas de salud e implementar nuevas investigaciones. Los gobiernos como Canadá, Estados Unidos Chile, Países Europeos y Asiáticos que han invertido cifras millonarias en Fluorización pública; ahora deben modificar sus programas e investigar daños causados. Por este motivo la dotación de agua deberá seguir estas directrices.

77 FR 23269 - Determination That FUNDUSCEIN-25 (fluorescein sodium injection), 25%, and AK-FLUOR (fluorescein...

Science.gov (United States)

2012-04-18

... marketing for reasons other than safety or effectiveness. ANDAs that refer to FUNDUSCEIN-25 (fluorescein...), 25%, Were Not Withdrawn From Sale for Reasons of Safety or Effectiveness AGENCY: Food and Drug... withdrawn from sale for reasons of safety or effectiveness. This determination will allow FDA to approve...

Data on the water balance in plants in the presence of fluor in the substrate

Energy Technology Data Exchange (ETDEWEB)

Navara, J

1969-01-01

Experiments were performed to determine the water balance of Pisum sativm to fluorine compounds. The results indicate that fluorine compounds in the substrate caused a withering of the above soil portions of the plants due to a disruption in the water balance. Water intake was generally observed to be reduced along with an increase in F concentration.

Hydroxyapatite, fluor-hydroxyapatite and fluorapatite produced via the sol-gel method. Optimisation, characterisation and rheology.

Science.gov (United States)

Tredwin, Christopher J; Young, Anne M; Georgiou, George; Shin, Song-Hee; Kim, Hae-Won; Knowles, Jonathan C

2013-02-01

Currently, most titanium implant coatings are made using hydroxyapatite and a plasma spraying technique. There are however limitations associated with plasma spraying processes including poor adherence, high porosity and cost. An alternative method utilising the sol-gel technique offers many potential advantages but is currently lacking research data for this application. It was the objective of this study to characterise and optimise the production of Hydroxyapatite (HA), fluorhydroxyapatite (FHA) and fluorapatite (FA) using a sol-gel technique and assess the rheological properties of these materials. HA, FHA and FA were synthesised by a sol-gel method. Calcium nitrate and triethylphosphite were used as precursors under an ethanol-water based solution. Different amounts of ammonium fluoride (NH4F) were incorporated for the preparation of the sol-gel derived FHA and FA. Optimisation of the chemistry and subsequent characterisation of the sol-gel derived materials was carried out using X-ray Diffraction (XRD) and Differential Thermal Analysis (DTA). Rheology of the sol-gels was investigated using a viscometer and contact angle measurement. A protocol was established that allowed synthesis of HA, FHA and FA that were at least 99% phase pure. The more fluoride incorporated into the apatite structure; the lower the crystallisation temperature, the smaller the unit cell size (changes in the a-axis), the higher the viscosity and contact angle of the sol-gel derived apatite. A technique has been developed for the production of HA, FHA and FA by the sol-gel technique. Increasing fluoride substitution in the apatite structure alters the potential coating properties. Crown Copyright © 2012. Published by Elsevier Ltd. All rights reserved.

RETENSI DAN INTRUNSI FLUOR PADA PERMUKAAN EMAIL SETELAH APLIKASI DENGAN SUBSTRAT IKAN TERI (Stolephorus sp.

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Harun Asyiq Gunawan

2015-08-01

Full Text Available Background: Topical fluoridation recently is still the main caries prevention method. Fluoride application on enamel surface is aimed to convert the hydroxyapatite (HA crystal to fluoroapatite (FA or hydroxyfluoroapatite (HFA form. Fluoride retention and intrusion onto enamel after fluoride application, therefore, are the most important factors to ensure the formation of FA and HFA crystals. Natural fluoride sources can be obtained from sea fishes, like teri (anchovy fish. Teri medan (S baganesis and teri jengki (S insularis are the most popular teri fishes in Indonesia, and they are known to have high fluoride content, 17-34 ppm. This research objectives are to measure fluoride retention and intrusion on enamel surface after application using teri medan and teri jengki substrate. Material and Method : 8 extracted third impacted molars were used. During the research, those teeth are immersed in ono-ionic aquadest (aquadem. Each tooth were cut bucco-lingually and mesio-distally, to obtain 4 enamel specimens. The specimens (totally 32 the divided into 3 major groups. 12 each for EDS group, and intrusion groups, and 8 for control. The EDS group divided into 2 sub-groups for application with teri medan and teri jengki. Similar procedure were done for intrusion and control groups. All EDS and intrusion specimens, then, immersed in the 5% teri substrate for 5 minutes, flushed with aquadem, then stored in aquadem for 3 hours. Procedure above then repeated 26 times. Control specimens were immersed in saline for 5 minutes, before flush and stored in the aquadem solution. All the EDS specimens, examinated using LEø Scanning and micro analysis electron microscope. Intrusion groups evaluated with Olypus BX41TF fluorescence microscope. Results : Fluoride retention for teri jengki groups is 5,28% and for teri medan group is 3,26%, while for control group is 1,94%. Fluoride intrusion for teri jengki group is 11,48 µm, teri medan 8,74 µm, and for control group is 0,84 µm. Anova and Bonferroni test, showed different result between experiment and control group, and between teri jengki and teri medan group, both for retention and intrusion examinations. Conclusion : Based in this research, it can be concluded that teri jengki and teri medan fishes substrate, give better result on fluoride retention and intrusion on enamel surface, compared to the control group. Application using teri jengki substrate gives better fluoride retention and intrusion on enamel surface compare to the teri medan substrate application.

Antigen processing of glycoconjugate vaccines; the polysaccharide portion of the pneumococcal CRM(197) conjugate vaccine co-localizes with MHC II on the antigen processing cell surface.

Science.gov (United States)

Lai, Zengzu; Schreiber, John R

2009-05-21

Pneumococcal (Pn) polysaccharides (PS) are T-independent (TI) antigens and do not induce immunological memory or antibodies in infants. Conjugation of PnPS to the carrier protein CRM(197) induces PS-specific antibody in infants, and memory similar to T-dependent (Td) antigens. Conjugates have improved immunogenicity via antigen processing and presentation of carrier protein with MHC II and recruitment of T cell help, but the fate of the PS attached to the carrier is unknown. To determine the location of the PS component of PnPS-CRM(197) in the APC, we separately labeled PS and protein and tracked their location. The PS of types 14-CRM(197) and 19F-CRM(197) was specifically labeled by Alexa Fluor 594 hydrazide (red). The CRM(197) was separately labeled red in a reaction that did not label PS. Labeled antigens were incubated with APC which were fixed, permeabilized and incubated with anti-MHC II antibody labeled green by Alexa Fluor 488, followed by confocal microscopy. Labeled CRM(197) was presented on APC surface and co-localized with MHC II (yellow). Labeled unconjugated 14 or 19F PS did not go to the APC surface, but PS labeled 14-CRM(197) and 19F-CRM(197) was internalized and co-localized with MHC II. Monoclonal antibody to type 14 PS bound to intracellular type 14 PS and PS-CRM(197). Brefeldin A and chloroquine blocked both CRM(197) and PS labeled 14-CRM(197) and 19F-CRM(197) from co-localizing with MHC II. These data suggest that the PS component of the CRM(197) glycoconjugate enters the endosome, travels with CRM(197) peptides to the APC surface and co-localizes with MHC II.

Fluoride Uptake Profiles of Selected European Toothpastes into Hard Tissues and Plaque.

Science.gov (United States)

Moore, Joel; Schneiderman, Eva; Farmer, Teresa; Zsiska, Marianne

2017-09-01

To compare the fluoridating potential of selected European toothpastes using a combination of enamel, dentin, and plaque in vitromodels. Four in vitromodels were included: 1) Enamel Fluoride (F) Uptake (EFU); 2) Dentin F Uptake (DFU); 3) Enamel Solubility Reduction (ESR); and 4) Plaque F Uptake (PFU). A core set of marketed products was included in all studies, plus a standard toothpaste (1100 ppm F as NaF/silica) and placebo control (the PFU study did not include a placebo control). Test dentifrices: [A] Fluocaril® Bi-Fluoré 250 (1500 ppm F as NaF+1000 ppm F as SMFP); [B] LacerAnticaries (2500 ppm F as SMFP); [C] Elmex® Caries Professional™ (1450 ppm F as SMFP+1.5% arginine); [D] Colgate® Triple Action (1450 ppm F as SMFP); [E] Placebo (0 ppm F); and [F] standard toothpaste (1100 ppm as NaF/silica). In all studies (EFU, DFU, ESR, and PFU), assessments were compared for each pair using the Tukey-Kramer HSD test (p F = B = C = D > E; DFU: A > F = B = C = D = E; PFU: A = B > F = C = D). In demineralization prevention, the Fluocaril dentifrice [A] also provided the greatest benefit (ESR: A = F = C = B = D > E). In all studies that included a placebo control, all of the F-containing dentifrices performed better than the placebo control. While these results demonstrate that all of the marketed products tested provide effective anticaries benefits, the Fluocaril Bi-Fluoré 250 dentifrice consistently delivered unsurpassed performance. It delivered the highest level of F to plaque, provided greater measures of efficacy in both remineralization and inhibition of demineralization, and delivered substantial improvement in fluoridation of dentin, suggesting the potential for delivering both coronal and root caries benefits.

Use of Complementary Approaches to Imaging Biomolecules and Endogenous and Exogenous Trace Elements and Nanoparticles in Biological Samples

Science.gov (United States)

Brown, Koshonna Dinettia

X-ray Fluorescence Microscopy (XFM) is a useful technique for study of biological samples. XFM was used to map and quantify endogenous biological elements as well as exogenous materials in biological samples, such as the distribution of titanium dioxide (TiO2) nanoparticles. TiO 2 nanoparticles are produced for many different purposes, including development of therapeutic and diagnostic particles for cancer detection and treatment, drug delivery, and induction of DNA breaks. Delivery of such nanoparticles can be targeted to specific cells and subcellular structures. In this work, we develop two novel approaches to stain TiO2 nanoparticles for optical microscopy and to confirm that staining by XFM. The first approach utilizes fluorescent biotin and fluorescent streptavidin to label the nanoparticles before and after cellular uptake; the second approach is based on the copper-catalyzed azide-alkyne cycloaddition, the so-called CLICK chemistry, for labeling of azide conjugated TiO2 nanoparticles with "clickable" dyes such as alkyne Alexa Fluor dyes with a high fluorescent yield. To confirm that the optical fluorescence signals of nanoparticles stained in situ match the distribution of the Ti element, we used high resolution synchrotron X-Ray Fluorescence Microscopy (XFM) using the Bionanoprobe instrument at the Advanced Photon Source at Argonne National Laboratory. Titanium-specific X-ray fluorescence showed excellent overlap with the location of Alexa Fluor optical fluorescence detected by confocal microscopy. In this work XFM was also used to investigate native elemental differences between two different types of head and neck cancer, one associated with human papilloma virus infection, the other virus free. Future work may see a cross between these themes, for example, exploration of TiO2 nanoparticles as anticancer treatment for these two different types of head and neck cancer.

[Study on application of CPP-ACP on tooth mineralization during orthodontic treatment with fixed appliance].

Science.gov (United States)

He, Wen-dan; Liu, Ying-zhi; Xu, Yuan-yuan; Chen, Dong

2010-04-01

To determine the value of clinical application of CPP-ACP during orthodontic treatment with fixed appliance. Seventy-five subjects were divided randomly into three groups, the control group, experimental group A and experimental group B. The control group were emphasized on daily oral hygiene. Experimental group A used fluor protector every three months under the dentist's guidance. Experimental group B used Casein phosphopeptide amorphous calcium phosphate(CCP-ACP) once a day. After finishing the orthodontic treatment, photos were taken under the same condition ,then the degree of the enamel's demineralization was examined and the enamel decalcification index(EDI) was calculated. SPSS11.0 software package was used for statistical analysis. (1)The incidence of three groups' enamel decalcification declined in sequence as the control group(60%),experimental group A (36%), experimental group B (32%).(2)The incidence of the teeth's enamel calcification of three groups declined in sequence as the control group(14.7%),experimental group A (8.46%), experimental group B (7.72%), the difference between the control group and two experimental groups was significant, while no significant difference was found between the two experimental groups.(3(EDI of the control group was 0.155+/-0.023, EDI of the experimental group A was 0.082+/-0.009,while EDI of the experimental group B was 0.078+/-0.006. The difference between the control group and two experimental groups was statistically significant, while was not statistically significant between the two experimental groups. The application of fluor protector and CPP-ACP can improve the mineralization of the teeth during orthodontic treatment with fixed appliance. CPP-ACP is more convenient for the patients to use. Supported by Research Fund of Bureau of Science and Technology of Futian District Shenzhen City(Grant No.FTWS056).

CLOSING IN ON CLOSURE PERSPECTIVES FROM HANFORD and FERNALD AN UPDATE

International Nuclear Information System (INIS)

CONNELL, J.D.

2004-01-01

In World War II, the arms dramatically changed from machine guns and incendiary bombs to nuclear weapons. Hanford and Fernald, two government-run sites, were part of the infrastructure established for producing the fissile material for making these weapons, as well as building a nuclear arsenal to deter future aggression by other nations. This paper compares and contrasts, from a communications point of view, these two Department of Energy (DOE) closure sites, each with Fluor as a prime contractor. The major differences between the two sites--Hanford in Washington state and Fernald in Ohio--includes the following: size of the site and the workforce, timing of closure, definition of end state, DOE oversight, proximity to population centers, readiness of local population for closure, and dependence of the local economy on the site's budget. All of these elements affect how the sites' communication professionals provide information even though the objectives are the same: build public acceptance and support for DOE's mission to accelerate cleanup, interface with stakeholders to help ensure that issues are addressed and goals are met, help workers literally work themselves out of jobs--faster, and prepare the ''host'' communities to deal with the void left when the sites are closed and the government contractors are gone. The 12-months between January 04 and January 05 have seen dramatic transformations at both sites, as Fernald is now just about a year away from closure and FLuor's work at Hanford has made the transition from operations to deactivation and demolition. While Fernald continues to clean out silos of waste and ship it off site, Hanford is dealing with recent state legislation that has the potential to significantly impact the progress of cleanup. These changes have even further accentuated the differences in the content, distribution, and impact of communications

Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes.

Science.gov (United States)

Telford, William G

2004-09-01

Violet laser diodes have become common and reliable laser sources for benchtop flow cytometers. While these lasers are very useful for a variety of violet and some ultraviolet-excited fluorochromes (e.g., DAPI), they do not efficiently excite most UV-stimulated probes. In this study, the next generation of InGaN near-UV laser diodes (NUVLDs) emitting in the 370-375-nm range have been evaluated as laser sources for cuvette-based flow cytometers. Several NUVLDs, ranging in wavelength from 370 to 374 nm and in power level from 1.5 to 10 mW, were mounted on a BD Biosciences LSR II and evaluated for their ability to excite cells labeled with the UV DNA binding dye DAPI, several UV phenotyping fluorochromes (including Alexa Fluor 350, Marina Blue, and quantum dots), and the fluorescent calcium chelator indo-1. NUVLDs at the 8-10-mW power range gave detection sensitivity levels comparable to more powerful solid-state and ion laser sources, using low-fluorescence microsphere beads as measurement standards. NUVLDs at all tested power levels allowed extremely high-resolution DAPI cell cycle analysis, and sources in the 8-10-mW power range excited Alexa Fluor 350, Marina Blue, and a variety of quantum dots at virtually the same signal-to-noise ratios as more powerful UV sources. These evaluations indicate that near-UV laser diodes installed on a cuvette-based flow cytometer performed nearly as well as more powerful solid-state UV lasers on the same instrumentation, and comparably to more powerful ion lasers on a jet-in-air system, and. Despite their limited power, integration of these small and inexpensive lasers into benchtop flow cytometers should allow the use of flow cytometric applications requiring UV excitation on a wide variety of instruments. Copyright 2004 Wiley-Liss, Inc.

Fuel cycle of nuclear power plants and safeguards system of nuclear weapon nonproliferation

International Nuclear Information System (INIS)

Malek, Z.

1980-10-01

The international safeguard system of nuclear weapon nonproliferation and the IAEA safeguard system are briefly described. In Czechoslovakia, a decree was issued in 1977 governing the accounting for and control of nuclear materials. The contents of the decree are presented. Described are computer processing of accounting data, technical criteria for the safeguard system application, containment and inspection in the IAEA safeguard system. The method is shown of the control of and accounting for nuclear materials in nuclear power plants and in fuel manufacturing, reprocessing and enrichment plants. Nondestructive and destructive methods of nuclear materials analysis are discussed. Nondestructive methods used include gamma spectrometry, neutron techniques, X-ray fluores--cence techniques. (J.P.)

The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae attachment clamps' sclerites and integumental proteins : research communication

Directory of Open Access Journals (Sweden)

S.J. Milne

2006-09-01

Full Text Available In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 µl WGA-TRX, 5 µl SYTO 9 and 5 µl of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms.

Complex logic functions implemented with quantum dot bionanophotonic circuits.

Science.gov (United States)

Claussen, Jonathan C; Hildebrandt, Niko; Susumu, Kimihiro; Ancona, Mario G; Medintz, Igor L

2014-03-26

We combine quantum dots (QDs) with long-lifetime terbium complexes (Tb), a near-IR Alexa Fluor dye (A647), and self-assembling peptides to demonstrate combinatorial and sequential bionanophotonic logic devices that function by time-gated Förster resonance energy transfer (FRET). Upon excitation, the Tb-QD-A647 FRET-complex produces time-dependent photoluminescent signatures from multi-FRET pathways enabled by the capacitor-like behavior of the Tb. The unique photoluminescent signatures are manipulated by ratiometrically varying dye/Tb inputs and collection time. Fluorescent output is converted into Boolean logic states to create complex arithmetic circuits including the half-adder/half-subtractor, 2:1 multiplexer/1:2 demultiplexer, and a 3-digit, 16-combination keypad lock.

Study of absorption and re-emission processes in a ternary liquid scintillation system

International Nuclear Information System (INIS)

Xiao Hualin; Wang Naiyan; Li Xiaobo; Cao Jun; Wen Liangjian; Zheng Dong

2010-01-01

Liquid scintillators are widely used as the neutrino target in neutrino experiments. The absorption and emission of different components of a ternary liquid scintillator (Linear Alkyl Benzene (LAB) as the solvent, 2, 5-diphenyloxazole (PPO) as the fluor and p-bis-(o-methylstyryl)-benzene (bis-MSB) as wavelength shifter) are studied. It is shown that the absorption of this liquid scintillator is dominant by LAB and PPO at wavelengths less than 349 nm, and the absorption by bis-MSB becomes prevalent at the wavelength larger than 349 nm. The fluorescence quantum yields, which are the key parameters to model the absorption and re-emission processes in large liquid scintillation detectors, are measured. (authors)

PNNL Apatite Investigation at 100-NR-2 Quality Assurance Project Plan

Energy Technology Data Exchange (ETDEWEB)

Fix, N. J.

2009-04-02

In 2004, the U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory (PNNL), and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at the 100-N Area would include apatite sequestration as the primary treatment, followed by a secondary treatment if necessary. Since then, the agencies have worked together to agree on which apatite sequestration technology has the greatest chance of reducing strontium-90 flux to the Columbia River. This Quality Assurance Project Plan provides the quality assurance requirements and processes that will be followed by staff working on the PNNL Apatite Investigation at 100-NR-2 Project. The plan is designed to be used exclusively by project staff.

Hyaluronic Acid in Normal and Neoplastic Colorectal Tissue: Electrospray Ionization Mass Spectrometric and Fluor Metric Analysis

Directory of Open Access Journals (Sweden)

Ana Paula Cleto Marolla

2016-01-01

Conclusions: The expression of HA was found to be slightly lower in tumor tissue than in colorectal non-neoplastic mucosa, although this difference was not statistically significant. This finding probably influenced the lower expression of HA in tumor tissue than in colorectal non-neoplastic mucosa. Compared to normal tissues, HA levels are significantly increased in the tumor tissues unless they exhibit lymph node metastasis. Otherwise, the expression of HA in tumor tissue did not correlated with the other clinicopathological parameters.

The effect of CPP-ACP containing fluoride on Streptococcus mutans adhesion and enamel roughness

Directory of Open Access Journals (Sweden)

Yulita Kristanti

2013-12-01

aplikasi CPP-ACP mengandung fluor sebelum dan setelah bleaching terhadap adhesi S.mutans dan kekasaran enamel. Metode: Sampel penelitian adalah 6 buah gigi yang dibagi dalam 4 kelompok, kemudian masing-masing gigi dibelah menjadi 4 bagian. Kelompok A dan C diaplikasi dengan CPP-ACP setelah bleaching, sedang Kelompok B dan D diaplikasi CPP-ACP sebelum dan setelah bleaching. CPP-ACP yang digunakan pada kelompok C dan D adalah yang mengandung fluor. Setelah perlakuan, semua sampel disterilkan dan direndam dalam saliva steril, lalu direndam dalam suspensi S. mutans 108 CFU dan diinkubasi 24 jam. Hari berikutnya sampel dimasukkan dalam BHI steril, divortex 1 menit untuk melepaskan bakteri. Lima belas ml BHI yang berisi S. mutans tersebut diambil untuk dikultur dalam agar TYS dan diinkubasi 37°C selama 48 jam. Bakteri yang tumbuh dihitung dengan colony counter. Pemeriksaan SEM dilakukan untuk meneliti permukaan enamel. Hasil: Aplikasi CPP-ACP(F menurunkan jumlah bakteri yang melekat pada enamel secara signifikan (p<0,05 pada semua kelompok, kecuali antara kelompok A dan C. Simpulan: Aplikasi CPP-ACP mengandung fluor sebelum dan sesudah bleaching efektif mengurangi akumulasi S. mutans dan kekasaran pada permukaan enamel.

Assessment of the Grouted IXC Monolith in Support of K East Basin Hazard Categorization

Energy Technology Data Exchange (ETDEWEB)

Short, Steven M.; Dodson, Michael G.; Alzheimer, James M.; Meyer, Perry A.

2007-10-12

Addendum to original report updating the structural analysis of the I-beam accident to reflect a smaller I-beam than originally assumed (addendum is 2 pages). The K East Basin currently contains six ion exchange columns (IXCs) that were removed from service over 10 years ago. Fluor Hanford plans to immobilize the six ion exchange columns (IXCs) in place in a concrete monolith. PNNL performed a structural assessment of the concrete monolith to determine its capability to absorb the forces imposed by postulated accidents and protect the IXCs from damage and thus prevent a release of radioactive material. From this assessment, design specifications for the concrete monolith were identified that would prevent a release of radioactive material for any of the postulated hazardous conditions.

Cherenkov and scintillation light separation in organic liquid scintillators

Energy Technology Data Exchange (ETDEWEB)

Caravaca, J.; Descamps, F.B.; Land, B.J.; Orebi Gann, G.D. [University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory, Berkeley, CA (United States); Yeh, M. [Brookhaven National Laboratory, Upton, NY (United States)

2017-12-15

The CHErenkov/Scintillation Separation experiment (CHESS) has been used to demonstrate the separation of Cherenkov and scintillation light in both linear alkylbenzene (LAB) and LAB with 2 g/L of PPO as a fluor (LAB/PPO). This is the first successful demonstration of Cherenkov light detection from the more challenging LAB/PPO cocktail and improves on previous results for LAB. A time resolution of 338 ± 12 ps FWHM results in an efficiency for identifying Cherenkov photons in LAB/PPO of 70 ± 3% and 63 ± 8% for time- and charge-based separation, respectively, with scintillation contamination of 36 ± 5% and 38 ± 4. LAB/PPO data is consistent with a rise time of τ{sub r} = 0.72 ± 0.33 ns. (orig.)

Neutron activation analysis of new botanical reference materials. Pt. 2

International Nuclear Information System (INIS)

Kucera, J.; Soukal, L.

1993-01-01

The certified, information, and other values of elemental contents were compared with results of neutron activation analysis (NAA) for the new Czechoslovak botanical reference materials (RMs) Green Algae 12-02-02, Lucerne 12-02-03, Wheat Bread Fluor 12-02-04, and Rye Bread Flour 12-02-05. These were prepared by the Institute of Radioecology and Applied Nuclear Techniques (IRANT), Kosice, and statistically evaluated after interlaboratory comparisons. For the majority of elements, a very good agreement was found between the IRANT values and the results of NAA. In several cases, however, significant differences were detected; possible analytical reasons for the differences and the suitability of a purely statistical evaluation of intercomparison results without analytical considerations for RM certification are discussed. (orig.)

The power of Indonesian coal

Energy Technology Data Exchange (ETDEWEB)

Rosiak, T. [Duke/Fluor Daniel (United States)

2003-02-01

The paper presents three Indonesian projects carried out by Duke/Fluor Daniel whose unique construction and operation have demonstrated the versatility and value of coal-fired power generation. These are: the construction of units 7 and 8 of the Paiton Private Power Project, a 1230 MW pulverised coal plant in Paiton, East Java; construction of a coal fired generation plant and transmission system to provide power for the expansion of a copper and gold mine on the island of Papua; and construction of four 28 MW pulverized coal units to provide 'heavy lifting' for a new mine at Batu Hijau on the island of Sumbawa. Coal was found to cost less than diesel for power generation. 2 photos.

Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium

International Nuclear Information System (INIS)

Wang Lei; Xu Guang; Shi Zhikun; Jiang Wei; Jin Wenrui

2007-01-01

We developed a sensitive single-molecule imaging method for quantification of protein by total internal reflection fluorescence microscopy with adsorption equilibrium. In this method, the adsorption equilibrium of protein was achieved between solution and glass substrate. Then, fluorescence images of protein molecules in a evanescent wave field were taken by a highly sensitive electron multiplying charge coupled device. Finally, the number of fluorescent spots corresponding to the protein molecules in the images was counted. Alexa Fluor 488-labeled goat anti-rat IgG(H + L) was chosen as the model protein. The spot number showed an excellent linear relationship with protein concentration. The concentration linear range was 5.4 x 10 -11 to 8.1 x 10 -10 mol L -1

Environmental Management Performance Report April 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-04-01

The purpose of the Environmental Management Performance Report (EMPR) is to provide the Department of Energy Richland Operations Office's (DOE-RL's) report of Hanford's Environmental Management (EM) performance by: Project Hanford Management Contract (PHMC) through Fluor Hanford, Inc. (FHI) and its subcontractors; Environmental Restoration Contract through Bechtel Hanford, Inc. (BHI), and its subcontractors; Pacific Northwest National Laboratories (PNNL) for EM and EM Science and Technology (S and T) Mission; and Office of Safety Regulation of the TWRS Privatization Contractor. This report is a monthly publication that summarizes EM Site performance under RL Operations Office. It is organized by the four sections listed above, with each section containing an Executive Summary and Area Performance Summaries.

The uranium of two 'private': from Jouac (Haute-Vienne) to Bertholene (Aveyron) through Saint-Pierre (Cantal)

International Nuclear Information System (INIS)

Guiollard, P.Ch.; Milville, G.

2003-01-01

In this book, the author tells us the short but intense history of some French uranium mines among the most famous ones: Jouac-Le Bernardan (Limousin) and Bertholene (Aveyron). It gives also a precious description of the discovery of the spar-fluor ore of La Charbonniere mine (Haute-Vienne) initially discovered thanks to its radioactivity (anthozonite bed) and which supplied a huge amount of fluorine. It presents the main companies (Scumra and Charbonnages du Dong-Trieu) which have exploited these deposits and also some less-known uranium mines of Aveyron, Creuse, Correze, Cantal and Limousin. The text is fully illustrated with cross sections and photos and gathers a large amount of geological and historical documents about mining techniques. (J.S.)

Cherenkov and scintillation light separation in organic liquid scintillators

International Nuclear Information System (INIS)

Caravaca, J.; Descamps, F.B.; Land, B.J.; Orebi Gann, G.D.; Yeh, M.

2017-01-01

The CHErenkov/Scintillation Separation experiment (CHESS) has been used to demonstrate the separation of Cherenkov and scintillation light in both linear alkylbenzene (LAB) and LAB with 2 g/L of PPO as a fluor (LAB/PPO). This is the first successful demonstration of Cherenkov light detection from the more challenging LAB/PPO cocktail and improves on previous results for LAB. A time resolution of 338 ± 12 ps FWHM results in an efficiency for identifying Cherenkov photons in LAB/PPO of 70 ± 3% and 63 ± 8% for time- and charge-based separation, respectively, with scintillation contamination of 36 ± 5% and 38 ± 4. LAB/PPO data is consistent with a rise time of τ r = 0.72 ± 0.33 ns. (orig.)

A fluorescence scanning electron microscope

International Nuclear Information System (INIS)

Kanemaru, Takaaki; Hirata, Kazuho; Takasu, Shin-ichi; Isobe, Shin-ichiro; Mizuki, Keiji; Mataka, Shuntaro; Nakamura, Kei-ichiro

2009-01-01

Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.

River Protection Project Integrated safety management system phase II verification review plan - 7/29/99

International Nuclear Information System (INIS)

SHOOP, D.S.

1999-01-01

The purpose of this review is to verify the implementation status of the Integrated Safety Management System (ISMS) for the River Protection Project (RPP) facilities managed by Fluor Daniel Hanford, Inc. (FDH) and operated by Lockheed Martin Hanford Company (LMHC). This review will also ascertain whether within RPP facilities and operations the work planning and execution processes are in place and functioning to effectively protect the health and safety of the workers, public, environment, and federal property over the RPP life cycle. The RPP ISMS should support the Hanford Strategic Plan (DOERL-96-92) to safely clean up and manage the site's legacy waste and deploy science and technology while incorporating the ISMS central theme to ''Do work safely'' and protect human health and the environment

Fernald incident underscores DOE cleanup woes

International Nuclear Information System (INIS)

Lobsenz, G.

1994-01-01

Miscalculations and poor safety planning led to a large release of deadly gas during an error-plagued effort to plug a leaking uranium hexafluoride canister discovered lying in a scrap heap at the Energy Department's Fernald plant last year, according to a DOE investigative report. Investigators with DOE's Office of Environment, Safety and Health said serious injury was avoided only because the wind happened to blow the toxic cloud of hydrogen fluoride gas away from inadequately protected Fernald workers watching the July 1993 canister-plugging operation at the Ohio plant. The investigators said the 25-minute canister repair effort - captured on videotape - was marked by poor planning by the Fernald Environmental Restoration Management Corp. (FERMCO), a Fluor Daniel subsidiary hired by DOE for its cleanup expertise

Microcircuit Device Reliability. Digital Evaluation and Failure Analysis Data. Parts 1 and 2, Summer 1980

Science.gov (United States)

1980-01-01

C - 7403 24 :0170C v V ISCTC :o IO a - TVYPCYC :-06r 50 ISO 1±8-U3 42: : 0: ,I~ 4 : I VCVC -2010C MOI- 0ST C"’IO C6SC -MS-883 - 42: 0: - - :V 96i’&u...FINE LK :HE 5.S-8 : MS- 8583 : 64: 60l 1 : : :V . :60 -h-1N : 101-4 A : . : :GkOSSLK :FLUOR 125C Ih-S-3 64: 0, * . : V : 31 : lu14 C : T.I. : E...5.4 . : : : 1 035110J SVARIOUS : DIP :5 .35/76 :.VIS INS :±150: : 0* ± 74122 ± 14 :0/70C : I : ± N.E. "" . . - :S4F E4 :025C 070C ISO :5± 0

Poly(amino acid) functionalized maghemite and gold nanoparticles

International Nuclear Information System (INIS)

Perego, Davide; Manuel Domínguez-Vera, José; Gálvez, Natividad; Masciocchi, Norberto; Guagliardi, Antonietta

2013-01-01

Bimodal MRI/OI imaging probes are of great interest in nanomedicine. Although many organic polymers have been studied thoroughly for in vivo applications, reports on the use of poly(amino acid)s as coating polymers are scarce. In this paper, poly-(d-glutamic acid, d-lysine) (PGL) has been used for coating maghemite and gold nanoparticles. An advantage of this flexible and biocompatible polymer is that, once anchored to the nanoparticle surface, dangling lysine amino groups are available for the incorporation of new functionalities. As an example, Alexa Fluor derivatives have been attached to PGL-coated maghemite nanoparticles to obtain magnetic/fluorescent materials. These dual-property materials could be used as bimodal MRI/OI probes for in vivo imaging. (paper)

Systems and methods for imaging using radiation from laser produced plasmas

Science.gov (United States)

Renard-Le Galloudec, Nathalie; Cowan, Thomas E.; Sentoku, Yasuhiko; Rassuchine, Jennifer

2009-06-30

In particular embodiments, the present disclosure provides systems and methods for imaging a subject using radiation emitted from a laser produced plasma generating by irradiating a target with a laser. In particular examples, the target includes at least one radiation enhancing component, such as a fluor, cap, or wire. In further examples, the target has a metal layer and an internal surface defining an internal apex, the internal apex of less than about 15 .mu.m, such as less than about 1 .mu.m. The targets may take a variety of shapes, including cones, pyramids, and hemispheres. Certain aspects of the present disclosure provide improved imaging of a subject, such as improved medical images of a radiation dose than typical conventional methods and systems.

Site Outcomes Baseline Multi Year Work Plan Volume 1, River Corridor Restoration Baseline

International Nuclear Information System (INIS)

Wintczak, T.M.

2001-01-01

The River Corridor Restoration volume is a compilation of Hanford Site scope, which excludes the approximately 194 km 2 Central Plateau. The River Corridor scope is currently contractually assigned to Fluor Hanford, Bechtel Hanford, inc., DynCorp, and Pacific Northwest National Laboratory, and others. The purpose of this project specification is to provide an overall scoping document for the River Corridor Restoration volume, and to provide a link with the overall Hanford Site River Corridor scope. Additionally, this specification provides an integrated and consolidated source of information for the various scopes, by current contract, for the River Corridor Restoration Baseline. It identifies the vision, mission, and goals, as well as the operational history of the Hanford Site, along with environmental setting and hazards

Origin and characterization of retrograde labeled neurons supplying the rat urethra using fiberoptic confocal fluorescent microscopy in vivo and immunohistochemistry.

Science.gov (United States)

Lee, Keon-Cheol; Sharma, Seema; Tuttle, Jeremy B; Steers, William D

2010-10-01

Autonomic innervation of urethral smooth muscle may influence urinary continence after prostatectomy. It is unclear whether the cavernous nerves carry fibers that influence continence. Using a retrograde axonal tracer combined with real-time in vivo imaging and ex vivo immunohistochemistry we determined the course and type of neurons supplying urethral smooth muscle distal to the prostate in the rat. We injected the retrograde axonal tracers cholera toxin B fragment-Alexa Fluor 488 and Fast Blue in the distal urethral smooth muscle in 10 rats each. Five days later the cavernous nerves and pelvic ganglion were imaged using fiberoptic confocal fluorescence microscopy (cholera toxin B fragment-Alexa Fluor 488) or harvested for immunohistochemistry (Fast Blue). Dual immunofluorescence of Fast Blue neurons with tyrosine hydroxylase or neuronal nitric oxide synthase was done to characterize neurons as noradrenergic or nitrergic. To ascertain whether the cavernous nerves contain fibers to the urethra that originate in the pelvic ganglia we cut the cavernous nerves with their ancillary branches in 3 rats and imaged them for Fast Blue. Fluorescent neurons and axons were detected in cavernous nerves and the pelvic ganglion. Few neurons were seen in rats with cavernous nerve section. Of urethral neurons 53.1% showed neuronal nitric oxide synthase positivity while 40.6% were immunoreactive for tyrosine hydroxylase. About 6.2% of urethral neurons failed to show tyrosine hydroxylase or neuronal nitric oxide synthase immunoreactivity. Most of the autonomic innervation to the urethra beyond the prostatic apex travels in the cavernous nerves. Many nerves may be parasympathetic based on neuronal nitric oxide synthase immunoreactivity. Nerves supplying the urethra outside the cavernous nerves may course posterior to the prostate. Along with afferent fibers, tyrosine hydroxylase immunoreactivity expressing neuron fibers, ie noradrenergic nerves, traveling in the cavernous nerves may

Role of ARF6 in internalization of metal-binding proteins, metallothionein and transferrin, and cadmium-metallothionein toxicity in kidney proximal tubule cells

International Nuclear Information System (INIS)

Wolff, Natascha A.; Lee, Wing-Kee; Abouhamed, Marouan; Thevenod, Frank

2008-01-01

Filtered metal-protein complexes, such as cadmium-metallothionein-1 (CdMT-1) or transferrin (Tf) are apically endocytosed partly via megalin/cubilin by kidney proximal tubule (PT) cells where CdMT-1 internalization causes apoptosis. Small GTPase ARF (ADP-ribosylation factor) proteins regulate endocytosis and vesicular trafficking. We investigated roles of ARF6, which has been shown to be involved in internalization of ligands and endocytic trafficking in PT cells, following MT-1/CdMT-1 and Tf uptake by PT cells. WKPT-0293 Cl.2 cells derived from rat PT S1 segment were transfected with hemagglutinin-tagged wild-type (ARF6-WT) or dominant negative (ARF6-T27N) forms of ARF6. Using immunofluorescence, endogenous ARF6 was associated with the plasma membrane (PM) as well as juxtanuclear and co-localized with Rab5a and Rab11 involved in early and recycling endosomal trafficking. Immunofluorescence staining of megalin showed reduced surface labelling in ARF6 dominant negative (ARF6-DN) cells. Intracellular Alexa Fluor 546-conjugated MT-1 uptake was reduced in ARF6-DN cells and CdMT-1 (14.8 μM for 24 h) toxicity was significantly attenuated from 27.3 ± 3.9% in ARF6-WT to 11.1 ± 4.0% in ARF6-DN cells (n = 6, P < 0.02). Moreover, reduced Alexa Fluor 546-conjugated Tf uptake was observed in ARF-DN cells (75.0 ± 4.6% versus 3.9 ± 3.9% of ARF6-WT cells, n = 3, P < 0.01) and/or remained near the PM (89.3 ± 5. 6% versus 45.2 ± 14.3% of ARF6-WT cells, n = 3, P < 0.05). In conclusion, the data support roles for ARF6 in receptor-mediated endocytosis and trafficking of MT-1/Tf to endosomes/lysosomes and CdMT-1 toxicity of PT cells

Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging.

Directory of Open Access Journals (Sweden)

Yoko Hayashi-Takanaka

Full Text Available To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ligands. However, little information is available for the suitability of different fluorescent dyes for live imaging. We here systematically analyzed the property of a number of commercial fluorescent dyes when conjugated with antigen-binding (Fab fragments directed against specific histone modifications, in particular, phosphorylated H3S28 (H3S28ph and acetylated H3K9 (H3K9ac. These Fab fragments were conjugated with a fluorescent dye and loaded into living HeLa cells. H3S28ph-specific Fab fragments were expected to be enriched in condensed chromosomes, as H3S28 is phosphorylated during mitosis. However, the degree of Fab fragment enrichment on mitotic chromosomes varied depending on the conjugated dye. In general, green fluorescent dyes showed higher enrichment, compared to red and far-red fluorescent dyes, even when dye:protein conjugation ratios were similar. These differences are partly explained by an altered affinity of Fab fragment after dye-conjugation; some dyes have less effect on the affinity, while others can affect it more. Moreover, red and far-red fluorescent dyes tended to form aggregates in the cytoplasm. Similar results were observed when H3K9ac-specific Fab fragments were used, suggesting that the properties of each dye affect different Fab fragments similarly. According to our analysis, conjugation with green fluorescent dyes, like Alexa Fluor 488 and Dylight 488, has the least effect on Fab affinity and is the best for live cell imaging, although these dyes are less photostable than red fluorescent dyes. When multicolor imaging is required, we recommend the following dye combinations for optimal results: Alexa Fluor 488 (green, Cy3 (red, and Cy5 or CF640 (far-red.

ASSET RECOVERY OF HAZARDOUS MATERIALS BENEFICIAL REUSE OF RADIOLOGICALLY ENCUMBERED LEAD STOCKS

International Nuclear Information System (INIS)

Lloyd, E.R.; Meehan, R.W.

2003-01-01

Underutilized and surplus lead stocks and leaded components are a common legacy environmental problem across much of the Department of Energy (DOE) Complex. While seeking to dispose of these items through its Environmental Management Program, DOE operational programs continue to pursue contemporary mission requirements such as managing and/or storing radioactive isotopes that require lead materials for shielding. This paradox was identified in late 1999 when DOE's policies for managing scrap metal were assessed. In January 2000, the Secretary of Energy directed the National Center of Excellence for Materials Recycle (NMR) to develop and implement a comprehensive lead reuse program for all of DOE. Fluor Hanford, contractor for DOE Richland Operations, subsequently contacted NMR to pilot lead reclamation and reuse at the Hanford Site. This relationship resulted in the development of a beneficial reuse pathway for lead reclaimed from spent fuel transport railcars being stored at Hanford. The 1.3 million pounds of lead in the railcars is considered radiologically encumbered due to its prior use. Further, the material was considered a mixed Resource Conservation and Recovery Act (RCRA) low-level radioactive waste that would require expensive storage or macro encapsulation to meet land disposal restrictions prior to burial. Working closely with Flour Hanford and the Office of Air, Water, and Radiation (EH-412), NMR developed a directed reuse pathway for this and other radiologically encumbered lead. When derived supplemental release limits were used, the lead recovered from these railcars became eligible for reuse in shielding products to support DOE and commercial nuclear industry operations. Using this disposition pathway has saved Hanford one third of the cost of disposing of the lead and the cost of acquiring additional lead for nuclear shielding applications. Furthermore, the environmental costs associated with mining and producing new lead for shielding products a

[Influence of the fluoride releasing dental materials on the bacterial flora of dental plaque].

Science.gov (United States)

Płuciennik, Małgorzata; Sakowska, Danuta; Krzemiński, Zbigniew; Piatowska, Danuta

2008-01-01

The assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed following manufacturers recommendations. Following materials were used: conventional glassionomer Ketac-Molar ESPE, resin modified glassionomer Fuji II LC GC and fluor containing composite Charisma Heraeus Kulzer Class V restorations were placed in following teeth of upper and lower jaw: canines, first bicuspids, second bicuspids. Sound enamel was a control. After 10 weeks the 72 hours old dental plaque was collected from surface of restorations and control using sterile probe. Total amount of 68 dental plaques were investigated. Each plaque was placed on scaled and sterile aluminum foil. The moist weight of dental plaque was scaled. Dental plaque was moved into 7 ml 0.85% NaCl solution reduced by cystein chlorine hydrogen and disintegrated by ultrasounds (power:100 Watt, wave amplitude: 5 micorm). The suspension of dental plaque was serially diluted from 10(-4) to 10(-5) in sterile 0,85% NaCl solution, and seeded with amount of 0.1 ml on appropriate base. In dental plaque trials the amount of cariogenic bacteria was calculated--Streptococcus mutans, Streptococcus, Lactobacillus, Veillonella and Neisseria, and also total amount of aerobic and anaerobic bacteria was measured. Microbiologic studies were performed in Institute of Microbiology, Medical University, Łódź. Statistical analysis of collected data was accomplished. In 72 hours old dental plaques collected from the surfaces of Ketac -Molar, Fuji II LC, Charisma after 10 weeks since being placed into the class V cavity, results show no statistically significant differences in the amount of Streptococcus mutans, Streptococcus spp., Lactobacillus spp., Veillonella spp., Neisseria spp, in total amount of aerobic and anaerobic bacteria and in the quantity proportion of Streptococcus mutans versus Streptococcus spp. in comparison

Development of an automatic smear sampler and a polymer film for surface radioactive contamination assay

International Nuclear Information System (INIS)

Seo, B.-K.; Lee, K.-W.; Woo, Z.-H.; Jeong, K.-S.; Oh, W.-Z.; Han, M.-J.

2004-01-01

Measurement of the surface contamination by an indirect method is subject to the various kinds of error according to the sampling person and needs much time and effort in the sampling and assay. In this research, an automatic smear sampler is developed. It improved efficiency for assay work of surface contamination level achieved periodically in a radiation controlled area. Using an automatic smear sampler developed, it is confirmed that radioactive contaminated materials are uniformly transferred to smear paper more than any sampling method by an operator. Also, Solid scintillation proximity membranes were prepared for measuring the amount of radioactive contamination in laboratories contaminated by the low energy beta-ray emitter, such as 3 H and 14 C. Polysulfone scintillation proximity membranes were prepared by impregnating Cerium Activated Yttrium Silicate (CAYS), an inorganic fluor, in a membrane structure. The inorganic fluor-impregnated membranes were applied to detect the radioactive surface contamination. The preparation of membranes was divided into two processes. A supporting polymer film was made of casting solutions consisting of polysulfone and solvent, their cast film being solidified by vacuum evaporation. CAYS-dispersed polymer solutions were cast over the first, solidified polymer films and coagulated either by evaporating solvent in the solution with non-solvent in a coagulation bath. The prepared membranes had two distinguished, but tightly attached, double layers : one is the supporting layer of dense polymer film and the other results revealed that the prepared membranes were efficient to monitor radioactive contamination with reliable counting ability. For enhancement of pick-up and measurement efficiency, the membrane was prepared with the condition of different membrane solidification. The scintillation produced by interaction with radiation and CAYS was measured with photomultiplier tube. The test results showed that the prepared

Urinary polycyclic aromatic hydrocarbon metabolites among 3-year-old children from Krakow, Poland.

Science.gov (United States)

Sochacka-Tatara, Elżbieta; Majewska, Renata; Perera, Frederica P; Camann, David; Spengler, John; Wheelock, Kylie; Sowa, Agata; Jacek, Ryszard; Mróz, Elżbieta; Pac, Agnieszka

2018-07-01

Polycyclic aromatic hydrocarbons (PAHs) are widespread in the environment and can adversely affect human health. The aim of the present study is to describe the level of PAHs exposure in children living in Kraków, one of the most polluted cities in Poland, and to determine the relationship of urinary biomarkers with environmental PAHsexposure. Urinary monohydroxy metabolites (OH-PAHs) of 20 PAHs were assessed in 218 three-year old children, of which only 10 were present in nearly all the samples: monohydroxy metabolites of naphthalene, fluorene, phenantrene and pyrene. Of the metabolites analyzed, hydroxynaphthalenes were predominant and constituted almost 73% of total excreted OH-PAHs, while 1-OH-PYRene was the least abundant (2.3% of total OH-PAHs). All measured urinary OH-PAHs were statistically significantly correlated with each other (R = 0.165-0.880) but the highest correlation coefficients with other individual OH-PAHs and with total OH-PAHs were observed for 2-OH-FLUOR. Children exposed at home to environmental tobacco smoke (ETS) had higher concentrations of fluorene and pyrene urinary metabolites compared to those without ETS exposure; and those exposed to gas-based appliances used for cooking or heating water had higher levels of fluorene and phenanthrene metabolites than children not exposed. The use of coal, wood or oil for heating was associated with elevated levels of 1-OH-PYRene. Urinary PAHs metabolites only modestly reflect high molecular weight carcinogenic PAHs exposures such as those monitored in air in the present study. None of the measured PAHs metabolites was correlated with airborne PM 2.5 and only two were slightly correlated with measured higher molecular mass airborne PAHs. The average concentrations of these specific metabolites in Polish children were much higher than observed in other pediatric populations living in developed countries. Our findings suggest that to capture various sources of PAHs, in addition to 1-OH

The deployment of an innovative real-time radiological soil characterization system

International Nuclear Information System (INIS)

Allen, David; Danahy, Raymond; Laird, Gregory; Seiller, Dale; White, Joan; Janke, Robert

2000-01-01

Fluor Fernald Inc., in conjunction with partners from Argonne National Laboratory, the Department of Energy's Environmental Measurements Laboratory, and Idaho National Engineering and Environmental Laboratory, has developed a program for characterizing radiological contaminants in soil in real time. The soil characterization system in use at the Fernald Environmental Management Project (FEMP) for over three years combines gamma ray spectrometry equipment with other technologies to produce a system that can scan large areas of ground and produce color coded maps which display quantitative information regarding isotopic contamination patterns. Software running on a battery powered lap-top computer, is used to control acquisition of gamma spectral data to link the spectral Information with precise detector position measurements from Global Positioning System (GPS) satellites, and to control transmission of data to a central station or van via a wireless Ethernet link where Surfer6 mapping software is used to produce maps showing the position and amount of each target analyte. Either sodium iodide (NaI) gamma ray detectors mounted on three different vehicles for mobile measurements or stationary tripod-mounted hyper-pure germanium (HPGe) detectors can be used in this system to radiologically characterize soil. The operational and performance characteristics, as well as the strengths and limitations of each of these units, will be described. The isotopic information generated by this system can be made available to remediation project mangers within an hour after the completion of a scan to aid in determination of excavation footprints, segregation of contaminated soil and verification of contamination removal. The immediate availability of radiological characterization data made possible by this real-time scanning system has allowed Fluor Fernald to accelerate remediation schedules and reduce costs by avoiding excavation delays and expensive and time consuming

Fluorescence quantum yields of natural organic matter and organic compounds: Implications for the fluorescence-based interpretation of organic matter composition

DEFF Research Database (Denmark)

Wünsch, Urban; Murphy, Kathleen R.; Stedmon, Colin

2015-01-01

to more than 200 modeled spectra (PARAFAC components) in the OpenFluor database. Apparent matches, based on spectral similarity, were subsequently evaluated using molar fluorescence and absorbance. Five organic compounds were potential matches with PARAFAC components from 16 studies; however, the ability......Absorbance and fluorescence spectroscopy are economical tools for tracing the supply, turnover and fate of dissolved organic matter (DOM). The colored and fluorescent fractions of DOM (CDOM and FDOM, respectively) are linked by the apparent fluorescence quantum yield (AQY) of DOM, which reflects...... the likelihood that chromophores emit fluorescence after absorbing light. Compared to the number of studies investigating CDOM and FDOM, few studies have systematically investigated AQY spectra for DOM, and linked them to fluorescence quantum yields (Φ) of organic compounds. To offer a standardized approach...

Direct fluorescence detection of VirE2 secretion by Agrobacterium tumefaciens

Science.gov (United States)

Yaakov, Noga; Barak, Yoav; Pereman, Idan; Christie, Peter J.; Elbaum, Michael

2017-01-01

VirE2 is a ssDNA binding protein essential for virulence in Agrobacterium tumefaciens. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging based on the ReAsH reagent. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretion chaperone VirE1. It was also biologically functional in complementing virE2 null strains transforming Arabidopsis thaliana roots and Nicotiana tabacum leaves. In vitro experiments demonstrated a two-color fluorescent complex using VirE2-TC/ReAsH and Alexa Fluor 488 labeled ssDNA. In vivo, fluorescent VirE2-TC/ReAsH was detected in bacteria and in plant cells at time frames relevant to transformation. PMID:28403156

Computational study of cation substitutions in apatites

International Nuclear Information System (INIS)

Tamm, Toomas; Peld, Merike

2006-01-01

Density-functional theory plane-wave modeling of fluor- and hydroxyapatites has been performed, where one or two calcium ions per unit cell were replaced with cadmium or zinc cations. It was found that cadmium ions favor Ca(1) positions in fluorapatites and Ca(2) positions in hydroxyapatites, in agreement with experiment. A similar pattern is predicted for zinc substitutions. In the doubly substituted cases, where only hydroxyapatites were modeled, a preference for the substituting ions to be located in Ca(2) position was also observed. Displacement of the hydroxide ions from their symmetrical positions on the hexagonal axis can be used to explain the preferred configurations of substituting ions around the axis. -- Deformation of the hydroxide ion chain due to substitutions around the ion channel in substituted hydroxyapatites

Environmental Management Performance Report for December 1999

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-02-16

The purpose of the Environmental Management Performance Report (EMPR) is to provide the Department of Energy Richland Operations Office's (DOE-RL's) report of Hanford's Environmental Management (EM) performance by: U. S. Department of Energy, Richland Operations Office, Project Hanford Management Contract (PHMC) through Fluor Hanford, Inc. (FHI) and its subcontractors, Environmental Restoration Contract through Bechtel Hanford, Inc. (BHI), and its subcontractors, and Pacific Northwest National Laboratories (PNNL) for EM and EM Science and Technology (S&T) Mission. This report is a monthly publication that summarizes EM Site performance under RL Operations Office. It is organized by the four sections listed above, with each section containing an Executive Summary and Area Performance Summaries. A listing of what is contained in the sections can be found in the Table of Contents.

Plan for the Initiation of HA-211 Furnace Operations at the Plutonium Finishing Plan (PFP)

International Nuclear Information System (INIS)

WILLIS, H.T.

2000-01-01

This plan provides a phased approach authorizing the use of three additional muffle furnaces for thermal stabilization. Achievement of Thermal Stabilization mission elements require the installation and startup of three additional muffle furnaces for the thermal stabilization of plutonium and plutonium bearing materials at the Plutonium Finishing Plant (PFP). The release to operate these additional furnaces will require an Activity Based Startup Review. The conduct of the Activity Based Startup Review (ABSR) was approved by Fluor Daniel Hanford on October 15, 1999. This plan has been developed with the objective of identifying those activities needed to guide the controlled startup of five furnaces from authorization to unrestricted operations by adding the HA-211 furnaces in an orderly and safe manner after the approval to Startup has been given

Treatment of obesity: an update on anti-obesity medications.

Science.gov (United States)

Halpern, A; Mancini, M C

2003-02-01

The information presented in this article provides an overview of physiological agents, therapeutics in current use, and medications that have been extensively used in the past but are no longer available, or are not classically considered as anti-obesity drugs. The authors present an extensive review on the criteria for anti-obesity management efficacy, on physiological mechanisms that regulate central and/or peripheral action energetic homeostasis (nutrients, monoamines and peptides), and on beta-phenethylamine pharmacological-derivative agents (fenfluramine, dexfenfluramine, phentermine, diethylpropion, fenproporex and sibutramine), tricyclic derivatives (mazindol), phenylpropanolamine derivatives (ephedrine, phenylpropanolamine), a phenylpropanolamine oxy-tri-fluor-phenyl derivative (fluoxetine), a naftilamine derivative (sertraline) and a lipstatine derivative (orlistat). An analysis of all clinical trials longer than 10 weeks in duration is also presented for medications used in the management of obesity.

Bismuth- and lithium-loaded plastic scintillators for gamma and neutron detection

International Nuclear Information System (INIS)

Cherepy, Nerine J.; Sanner, Robert D.; Beck, Patrick R.; Swanberg, Erik L.; Tillotson, Thomas M.; Payne, Stephen A.; Hurlbut, Charles R.

2015-01-01

Transparent plastic scintillators based on polyvinyltoluene (PVT) have been fabricated with high loading of bismuth carboxylates for gamma spectroscopy, and with lithium carboxylates for neutron detection. When activated with a combination of standard fluors, 2,5-diphenyloxazole (PPO) and tetraphenylbutadiene (TPB), gamma light yields with 15 wt% bismuth tripivalate of 5000 Ph/MeV are measured. A PVT plastic formulation including 30 wt% lithium pivalate and 30 wt% PPO offers both pulse shape discrimination, and a neutron capture peak at ~400 keVee. In another configuration, a bismuth-loaded PVT plastic is coated with ZnS( 6 Li) paint, permitting simultaneous gamma and neutron detection via pulse shape discrimination with a figure-of-merit of 3.8, while offering gamma spectroscopy with energy resolution of R(662 keV)=15%

The Porgera gold deposit, Papua, New Guinea, 1: association with alkalic magmatism in a continent-island-arc collision zone

International Nuclear Information System (INIS)

Richards, J.P.; Chappell, B.W.; McCulloch, M.T.; McDougall, I.

1991-01-01

The meso thermal to epithermal Porgera gold deposit is spatially and temporally associated with shallow level (≤ 2 km emplacement depth) stocks and dykes of the Porgera Intrusive Complex (PIC). Gold mineralization immediately followed emplacement of the PIC, and is dated between 5 and 6 Ma ago. The Porgera intrusive suite is comprised of fine- to medium-grained, porphyritic to euhedral granular, volatile-rich, sodic alkali basalts/gabbros, hawaiites, and mugearites (TAS chemical classification scheme). The rocks display chemical and isotopic characteristics similar to those of intra plate alkalic basalts, but their unusually high volatile contents result in stabilization of hornblende as a phenocryst and intergranular phase in more evolved rock types. The observed order of cotectic crystallization is olivine - clinopyroxene - hornblende -plagioclase, with ubiquitous spinel (chromite/magnetite) and fluor-apatite. (author)

FFTF Authorization Agreement

International Nuclear Information System (INIS)

DAUTEL, W.A.

2000-01-01

The purpose of the Authorization Agreement is to serve as a mechanism whereby the U.S. Department of Energy, Richland Operations Office (RL) and Fluor Hanford (FH) jointly clarify and agree to key conditions for conducting work safely and efficiently in the Fast Flux Test Facility (FFTF). Work must be accomplished in a manner that achieves high levels of quality while protecting the environment and the safety and health of workers and the public, and complying with applicable contractual and regulatory requirements. It is the intent of this Agreement to address those items of significant importance in establishing and supporting the FFTF Authorization Envelope, but this Agreement in no way alters the terms and conditions of the Project Hanford Management Contract (PHMC), Contract Number DE-AC06-96RL13200

Environmental Management Performance Report November 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-11-01

The purpose of the Environmental Management Performance Report (EMPR) is to provide the Department of Energy Richland Operations Office's (DOE-RL's) report of Hanford's Environmental Management (EM) performance by: Project Hanford Management Contract (PHMC) through Fluor Hanford, Inc. (FH) and its subcontractors; Environmental Restoration Contract through Bechtel Hanford, Inc. (BHI), and its subcontractors; Pacific Northwest National Laboratories (PNNL) for Science and Technology support to the EM Mission; and Office of Safety Regulation of the TWRS Privatization Contractor. This report is a monthly publication that summarizes EM Site performance under RL Operations Office. It is organized by the four sections listed above, with each section containing an Executive Summary and Area Performance Summaries. A glossary of terms is provided at the end of this report for reference purposes.

[Influence of dental caries prophylaxis using fluor-vigantoletten 1000 on plasma fluoride levels in infants (author's transl)].

Science.gov (United States)

Fuchs, C; Pohlandt, F; Abigt, J; Töllner, U; Heimann, G

1979-04-01

A group of 57 newborns was divided into two: group I (27 subjects) received solely 1,000 I.U. vitamin D3 per day for 6 months, whereas group II (30 subjects) were given a combination of 1,000 I.U. vitamin D3 and 0.25 mg fluoride. At the end of the time of observation there was a significant difference between the plasma concentrations in the two groups. The median value was 13.06 microgram/l (range: 8:18-39.4 microgram/l) in group I and 16.54 microgram/l (range: 9.9--41.2 microgram/l) in group II. Fluoride is obviously available to infants when administered in combination with Vitamin D3. In addition, it could be proven, that after 6 month administration of fluoride there are no signs of cumulation, overdosage or even intoxication.

On the synthesis of radiofluorinated amino acids by isotope exchange based on the example of 6-[{sup 18}F]Fluor-L-DOPA; Zur Synthese radiofluorierter aromatischer Aminosaeuren mittels Isotopenaustausch am Beispiel von 6-[{sup 18}F]Fluor-L-DOPA

Energy Technology Data Exchange (ETDEWEB)

Wagner, F M

2008-06-15

In nuclear medical diagnosis, 6-[{sup 18}F]fluoro-L-3,4-dihydroxyphenylalanine (6-[{sup 18}F]fluoro-LDOPA), an analogue of L-DOPA, is one of the few established radiopharmaceuticals used for the in vivo investigation of the presynaptic dopaminergic metabolism and of some kind of tumours via Positron Emission Tomography (PET). The presently used method of preparation of the radiotracer by electrophilic labelling is limited to low amounts of activity at high costs. Known nucleophilic syntheses, however, result either in insufficient enantiomeric purity or the known multi-step syntheses are hard to automate, due to their complexity. During this work a novel, easy to automate alternative for the preparation of 6-[{sup 18}F]fluoro-L-DOPA, was developed and evaluated, using a direct nucleophilic {sup 18}F-fluorination of a protected amino acid derivative. The resulting product has a very high enantiomeric purity. At first, the general suitability of the (S)-BOC-BMI-derivatives for the synthesis of {sup 18}F-labelled amino acids, used in this work, was investigated using a less complex precursor, which resulted in the amino acid 6-[{sup 18}F]fluoro-L-m-tyrosin via acidic hydrolysis. The preparation of a useful precursor for the nucleophilic {sup 18}F-isotope substitution, namely the (2S,5S)-tert.-butyl- 5-(2-fluoro-5-formylbenzyl)-2-tert.-butyl-3-methyl-4-oxoimidazolidine-1-carboxylate, was investigated in three general different ways. At first it was tried to obtain this product via formylation after coupling with the BOC-BMI, secondly via {alpha},{beta}-dehydro amino acid derivatives and finally via a systematic multi-step synthesis. Only the last mentioned way resulted in a precursor with sufficient purity that could be labelled. The radiochemical yield of the isotopic exchange was about 60 %. In the next step, the presented concept was modified to synthesize a precursor for the preparation of 6-[{sup 18}F]fluoro-L-DOPA. Only a combination of the protecting groups benzyl and THP resulted in the useful precursor (2S,5S)-tert.-butyl-5-(4-benzyloxy-2-fluoro-5- formylbenzyl)-2-tert.-butyl-3-methyl-4-oxoimidazolidine-1-carboxylate. By optimisation of the isotopic exchange with [{sup 18}F]fluoride on this precursor a radiochemical yield of about 50 % was achieved. After a three step synthesis including the labelling, a Baeyer-Villiger-oxidation using mCPBA and a hydrolysis using HBr, c.a. 6-[{sup 18}F]fluoro-L-DOPA was isolated containing more than 99 % of the desired L-isomer. The complete preparation and isolation of c.a. 6-[{sup 18}F]fluoro-L-DOPA, using optimised conditions, resulted in a decay corrected yield of about 22 % after a synthesis time of 105 minutes. Thus the presented synthesis of 6-[{sup 18}F]fluoro-L-DOPA is not only more efficient than the known methods of preparation, but is furthermore fully automatable. (orig.)

Optimization of protein samples for NMR using thermal shift assays

International Nuclear Information System (INIS)

Kozak, Sandra; Lercher, Lukas; Karanth, Megha N.; Meijers, Rob; Carlomagno, Teresa; Boivin, Stephane

2016-01-01

Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20 °C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays or ThermoFluor"® provide a high-throughput screening method to assess the thermal stability of a sample under several conditions simultaneously. Here, we describe a thermal shift assay that is designed to optimize conditions for nuclear magnetic resonance studies, which typically require stable samples at high concentration and ambient (or higher) temperature. We demonstrate that for two challenging proteins, the multicomponent screen helped to identify ingredients that increased protein stability, leading to clear improvements in the quality of the spectra. Thermal shift assays provide an economic and time-efficient method to find optimal conditions for NMR structural studies.

Optimization of protein samples for NMR using thermal shift assays

Energy Technology Data Exchange (ETDEWEB)

Kozak, Sandra [European Molecular Biology Laboratory (EMBL), Hamburg Outstation, SPC Facility (Germany); Lercher, Lukas; Karanth, Megha N. [European Molecular Biology Laboratory (EMBL), SCB Unit (Germany); Meijers, Rob [European Molecular Biology Laboratory (EMBL), Hamburg Outstation, SPC Facility (Germany); Carlomagno, Teresa, E-mail: teresa.carlomagno@oci.uni-hannover.de [European Molecular Biology Laboratory (EMBL), SCB Unit (Germany); Boivin, Stephane, E-mail: sboivin77@hotmail.com, E-mail: s.boivin@embl-hamburg.de [European Molecular Biology Laboratory (EMBL), Hamburg Outstation, SPC Facility (Germany)

2016-04-15

Maintaining a stable fold for recombinant proteins is challenging, especially when working with highly purified and concentrated samples at temperatures >20 °C. Therefore, it is worthwhile to screen for different buffer components that can stabilize protein samples. Thermal shift assays or ThermoFluor{sup ®} provide a high-throughput screening method to assess the thermal stability of a sample under several conditions simultaneously. Here, we describe a thermal shift assay that is designed to optimize conditions for nuclear magnetic resonance studies, which typically require stable samples at high concentration and ambient (or higher) temperature. We demonstrate that for two challenging proteins, the multicomponent screen helped to identify ingredients that increased protein stability, leading to clear improvements in the quality of the spectra. Thermal shift assays provide an economic and time-efficient method to find optimal conditions for NMR structural studies.

Preservation of rodent bones from El Harhoura 2 cave (Morocco, Neolithic - Middle Palaeolithic): Microstructure, mineralogy, crystallinity and composition

Science.gov (United States)

Farre, Bastien; Massard, Pierre; Nouet, Julius; Dauphin, Yannicke

2014-04-01

Thin sections, scanning electron microscopy (SEM), diffraction X (DRX) and infrared spectrometry (FTIR) have been used to study the structure, mineralogy, crystallinity and bulk composition of fossil rodent long bones extracted from a succession of sedimentary layers in a cave from Morocco (Neolithic - Middle Palaeolithic, El Harhoura 2). The microstructure of fossil bones is well-preserved at this scale of observation, and encrusted deposits are rare. All bones are preserved in apatite, but the crystallinity is modified, as well as the crystallite shape, the organic content and the organic-mineral ratio. No fluor enrichment has been observed. Alone or together, the studied parameters do not show a regular trend from the upper to the lower layers of the cave. The preservation of the fossil bones does not confirm the sequence of arid and humid periods inferred from taphonomic analyses.

NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS (NESHAP) SUBPART H RADIONUCLIDES POTENTIAL TO EMIT CALCULATIONS

Energy Technology Data Exchange (ETDEWEB)

EARLEY JN

2008-07-23

This document provides an update of the status of stacks on the Hanford Site and the potential radionuclide emissions, i.e., emissions that could occur with no control devices in place. This review shows the calculations that determined whether the total effective dose equivalent (TEDE) received by the maximum public receptor as a result of potential emissions from any one of these stacks would exceed 0.1 millirem/year. Such stacks require continuous monitoring of the effluent, or other monitoring, to meet the requirements of Washington Administrative code (WAC) 246-247-035(1)(a)(ii) and WAC 246-247-075(1), -(2), and -(6). This revised update reviews the potential-to-emit (PTE) calculations of 31 stacks for Fluor Hanford, Inc. Of those 31 stacks, 11 have the potential to cause a TEDE greater than 0.1 mrem/year.

NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS (NESHAP) SUBPART H; RADIONUCLIDES POTENTIAL-TO-EMIT CALCULATIONS

International Nuclear Information System (INIS)

EARLEY JN

2008-01-01

This document provides an update of the status of stacks on the Hanford Site and the potential radionuclide emissions, i.e., emissions that could occur with no control devices in place. This review shows the calculations that determined whether the total effective dose equivalent (TEDE) received by the maximum public receptor as a result of potential emissions from any one of these stacks would exceed 0.1 millirem/year. Such stacks require continuous monitoring of the effluent, or other monitoring, to meet the requirements of Washington Administrative code (WAC) 246-247-035(1)(a)(ii) and WAC 246-247-075(1), -(2), and -(6). This revised update reviews the potential-to-emit (PTE) calculations of 31 stacks for Fluor Hanford, Inc. Of those 31 stacks, 11 have the potential to cause a TEDE greater than 0.1 mrem/year

Minerals from Morvan; Les mineraux du Morvan

Energy Technology Data Exchange (ETDEWEB)

Bayle, L.D

2007-07-01

Morvan is a mountainous and wooded region of Bourgogne (Burgundy, France) which represents the NE end of the French Massif central. The geologic history of this area has been propitious to the setting up of numerous ore deposits: iron, fluor, manganese, lead, barium and uranium. Morvan is the cradle of the French uranium industry. This book presents, first, the geology, geodynamics and ore deposits of Morvan, the history of the discovery of autunite and the exploitation of uranium. The three uranium mining districts are reviewed with the list of uranium minerals. The uranium exploitation activity was abandoned in 1990. An overview of a dozen of fluorine, barytine, lead and silver-bearing ore deposits is presented as well. A summary of all other mineral species associated to the granitic, metamorphic and volcanic areas of the region is proposed. (J.S.)

[Assessment of periodontal condition of kidney patients in hemodialysis].

Science.gov (United States)

Souza, Cláudia Régia Dias de; Libério, Silvana Amado; Guerra, Rosane Nassar Meireles; Monteiro, Silvio; Silveira, Ericka Janine Dantas da; Pereira, Antonio Luiz Amaral

2005-01-01

Thirty patients undergoing hemodialysis were evaluated for their periodontal condition through plaque, calculus and gingival indexes; PSR and IgA present in the saliva were also appraised in order to determine the periodontal condition of patients presenting chronic kidney disease. Results obtained demonstrated that in the studied group the plaque, calculus and gingival indexes were high. With regard to these patients' requirement to undergo periodontal treatment, most patients submitted to hemodialysis needed better oral hygiene, in addition to supra and sub gingival scraping and topic application of fluor (code 2). As to the IgA amount present in the saliva, only three patients showed a low density. Patients presenting chronic kidney disease disclosed a tendency for greater bacterial plaque concentration, high formation of dental calculus suggesting the need for periodontal treatment comprising supra and sub gingival scraping.

Artificial caries formation around fluoride-releasing restorations in roots.

Science.gov (United States)

Dionysopoulos, P; Kotsanos, N; Papadogiannis, Y; Konstantinidis, A

1998-11-01

Secondary caries is one of the most important factors leading to replacement of dental restorations. This investigation assessed the capacity of fluoride-releasing restorative materials to resist caries in vitro when used in roots. Class 5 cavities were prepared in the buccal and lingual surfaces of 30 extracted premolars. The six materials used were: glass-ionomer cement (Fuji), glass-ionomer cement with silver particles added (Ketac-silver), fluoride-containing composite resin (Tetric), composite resin (Silux plus), fluoride-containing amalgam (Fluor-Alloy) and high-copper amalgam (Dispersalloy). After 5 weeks in an acid gel for caries-like lesion formation, the teeth were sectioned longitudinally and examined with polarized light. The results showed that repair with glass-ionomer materials of a carious lesion may be of great importance in the prevention of secondary caries around the restorations in roots.

Recycle Waste Collection Tank (RWCT) simulant testing in the PVTD feed preparation system

International Nuclear Information System (INIS)

Abrigo, G.P.; Daume, J.T.; Halstead, S.D.; Myers, R.L.; Beckette, M.R.; Freeman, C.J.; Hatchell, B.K.

1996-03-01

(This is part of the radwaste vitrification program at Hanford.) RWCT was to routinely receive final canister decontamination sand blast frit and rinse water, Decontamination Waste Treatment Tank bottoms, and melter off-gas Submerged Bed Scrubber filter cake. In order to address the design needs of the RWCT system to meet performance levels, the PNL Vitrification Technology (PVTD) program used the Feed Preparation Test System (FPTS) to evaluate its equipment and performance for a simulant of RWCT slurry. (FPTS is an adaptation of the Defense Waste Processing Facility feed preparation system and represents the initially proposed Hanford Waste Vitrification Plant feed preparation system designed by Fluor-Daniel, Inc.) The following were determined: mixing performance, pump priming, pump performance, simulant flow characterization, evaporator and condenser performance, and ammonia dispersion. The RWCT test had two runs, one with and one without tank baffles

The treatment of large quantities of high fluorin contents UO2 by ammonium double uranate (ADU) techniques

International Nuclear Information System (INIS)

Wang Bangwu; Chen Ying

2010-01-01

The paper has discussed the sinter action of UO 2 in low temperature. The study indicates the over hot part of UO 2 by the deoxidization hot of oxidation uranate mostly results in the sinter in the process of trans form ADU into UO 2 . The UO 2 settling times in kiln little influences the sinter performance of UO 2 in the same condition of high fluorin contents UO 2 returning kiln, and high fluorin contents UO 2 returning kiln does not sinter UO 2 again. Experiment on large quantities of high fluorin contents UO 2 by Ammonium Double Uranate (ADU) techniques direct returning kiln, the result shows the sinter performance of UO 2 doesn't drop in the process of high fluor in contents UO 2 direct returning kiln, and the performance of UO 2 can meet specification. (authors)

XMM-Newton Proposal 03039101

Science.gov (United States)

Hernanz, Margarita

2004-10-01

Two recent galactic novae, V2487 Oph 1998 and V4633 Sgr 1998, have been detec- ted in both soft and hard X-rays with XMM. V2487 Oph showed an iron fluores- cent line only 2.7 yrs after its explosion, a clear signature of the very fast recovery of accretion, and V4633 Sgr displayed hard X-ray emission not clearly attributable to shocked ejecta or accretion. Longer observations are needed to do accurate timing and high resolution spectroscopy, essential to determine the main properties of the underlying cataclysmic variable in V2487 Oph (first nova seen in X-rays prior and after exploding) and to disentangle the origin of the hard X-rays from V4633 Sgr, through an analysis of the chemical compo- sition of the emitting thermal plasma, solar if accretion, non solar if ejecta.

Kinetics of selected elementary reactions of NH(a{sup 1}{delta}) or ND(a{sup {delta}}), NH(X{sup 3}{sigma}{sup -}) or ND(X{sup 3}{sigma}{sup -}) and NH{sub 2}(X), NHD(X), NH{sub 2}(X) radicals; Untersuchung der Kinetik ausgewaehlter Elementarreaktionen von NH(a{sup 1}{delta})- bzw. ND(a{sup 1}{delta})-, NH(X{sup 3}{sigma}{sup -})- bzw. ND(X{sup 3}{sigma}{sup -})- und NH{sub 2}(X)-, NHD(X)-, ND{sub 2}(X)-Radikalen

Energy Technology Data Exchange (ETDEWEB)

Adam, L.

2002-02-01

The elementary reactions of the NH and ND radicals in the ground state and the first excited state with H({sup 2}S) atoms and with molecules containing hydrogen and fluorine element bonds were investigated in the gaseous phase at a pressure of p = 7-80 bar. The elementary reactions of the NH{sub 2}, NHD and ND{sub 2} radicals in the ground state with hydrogen halides in the gaseous phase were investigated as well. [German] Die Elementarreaktionen des NH- bzw. ND-Radikals im Elektronengrundzustand und im ersten elektronisch angeregten Zustand mit H({sup 2}S)-Atomen und mit Molekuelen, die Wasserstoff- und Fluor-Elementbindungen besitzen, wurden in der Gasphase bei einem Druck von p = 7 - 80 mbar untersucht. Weiterhin wurden die Elementarreaktionen der NH{sub 2}-, NHD- bzw. ND{sub 2}-Radikale im Elektronengrundzustand mit Halogenwasserstoffen in der Gasphase untersucht. (orig.)

200 Area plateau inactive miscellaneous underground storage tanks locations

International Nuclear Information System (INIS)

Brevick, C.H.

1997-01-01

Fluor Daniel Northwest (FDNW) has been tasked by Lockheed Martin Hanford Corporation (LMHC) to incorporate current location data for 64 of the 200-Area plateau inactive miscellaneous underground storage tanks (IMUST) into the centralized mapping computer database for the Hanford facilities. The IMUST coordinate locations and tank names for the tanks currently assigned to the Hanford Site contractors are listed in Appendix A. The IMUST are inactive tanks installed in underground vaults or buried directly in the ground within the 200-East and 200-West Areas of the Hanford Site. The tanks are categorized as tanks with a capacity of less than 190,000 liters (50,000 gal). Some of the IMUST have been stabilized, pumped dry, filled with grout, or may contain an inventory or radioactive and/or hazardous materials. The IMUST have been out of service for at least 12 years

[Summary of the practice guideline 'Vaginal discharge' (first revision) from the Dutch College of General Practitioners].

Science.gov (United States)

Boukes, F S; Boeke, A J P; Dekker, J H; Wiersma, T; Goudswaard, A N

2007-06-16

The 1996 practice guideline of the Dutch College of General Practitioners (NHG) on vaginal discharge has been updated. Most women who visit their doctor with complaints about vaginal discharge do not have an increased risk of a sexually-transmitted disease. Investigations into vaginal discharge comprise history taking, physical examination and microscopic analysis in the laboratory of the general practitioner. Additional investigation into Chlamydia, gonorrhoea and Trichomonas infection is only necessary if the patient history reveals an increased risk of a sexually-transmitted disease. A Candida infection or bacterial vaginosis should only be treated if the patient experiences bothersome complaints. Treatment of a Candida infection consists of a vaginally applied imidazole compound. Bacterial vaginosis can be treated with oral administration of metronidazole. Patients with vaginal fluor can be examined and, if necessary, treated by their general practitioner. Referral to a gynaecologist is rarely necessary.

Underground transmission tomography

International Nuclear Information System (INIS)

Geibka, C.

1990-01-01

Several underground tomographic transmission surveys have been carried out. Targets were cavities, ore veins and fault zones. Examples from measurements in a german heavy/fluor spar mine a lead/zinc mine and a rock laboratory of the Swiss National Cooperative for the Storage of Radioactive waste are presented. Measurements were carried out between boreholes and road ways. The recording equipment was the intrinsically safe SEAMEX85 system built and sold by WBK. Receivers were mounted in a chain of 6 two-component probes. Sources were an inhole hammer a sledge hammer a sparker and explosives from a single detonator to 180 g depending on the distance and absorption of the rock material. Cavities showed very distinct velocity reductions between 30 and 50%. Different vein material showed velocity reduction as well as velocity increase relative to the surrounding rock

The Identification of Aluminum in Human Brain Tissue Using Lumogallion and Fluorescence Microscopy

Science.gov (United States)

Mirza, Ambreen; King, Andrew; Troakes, Claire; Exley, Christopher

2016-01-01

Aluminum in human brain tissue is implicated in the etiologies of neurodegenerative diseases including Alzheimer’s disease. While methods for the accurate and precise measurement of aluminum in human brain tissue are widely acknowledged, the same cannot be said for the visualization of aluminum. Herein we have used transversely-heated graphite furnace atomic absorption spectrometry to measure aluminum in the brain of a donor with Alzheimer’s disease, and we have developed and validated fluorescence microscopy and the fluor lumogallion to show the presence of aluminum in the same tissue. Aluminum is observed as characteristic orange fluorescence that is neither reproduced by other metals nor explained by autofluorescence. This new and relatively simple method to visualize aluminum in human brain tissue should enable more rigorous testing of the aluminum hypothesis of Alzheimer’s disease (and other neurological conditions) in the future. PMID:27472886

The radon hazard in non - uranium European mines: Retrospective of a survey conducted between 1978 and 1982 in different mines across Europe and new results in France

International Nuclear Information System (INIS)

Bernhard, S.; Pineau, J.F.; Zettwoog, P.; Skowronek, J.

1996-01-01

From 1979 to 1982, a study of the hazard due to the natural radioactivity in non-uranium mines has been initiated by the CEC. The programme of the work involved coal, potash salt, fluors par, gypsum, clay, graphite, tungsten, antimony, bauxite, cassiterite, slate, iron, pyrites, lead-zinc, tin, silver-zinc, as well as gold mines, situated in Belgium, France, United Kingdom, Germany and Italy. In all studies mines, a radiological and ventilation audit have been conducted and an individual dosimetry has been implemented for a group of a few tens of miners in some of the mines. An overview of the results is presented here, giving information about the radiological risk treatming the miner's health. The overview is completed with recent measurements made more recently, on a routine basis, in some French non-uranium mines. (author). 47 refs, 8 figs, 3 tabs

Optimization of the wavelength shifter ratio in a polystyrene based plastic scintillator through energy spectrum analysis

Energy Technology Data Exchange (ETDEWEB)

Kim, Ye Won; Kim, Myung Soo; Yoo, Hyun Jun; Lee, Dae Hee; Cho, Gyu Seong [Dept. of Nuclear and Quantum Engineering, KAIST, Daejeon (Korea, Republic of); Moon, Myung Kook [Neutron Instrumentation Division, Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

2017-02-15

The scintillation efficiency of the polystyrene based plastic scintillator depends on the ratio of the wavelength shifters, organic fluors (PPO and POPOP). Thus, 24 samples of the plastic scintillator were fabricated in order to find out the optimum ratio of the wavelength shifters in the plastic scintillator. The fabricated plastic scintillators were trimmed through a cutting and polishing process. They were used in gamma energy spectrum measurement with the {sup 137}Cs emitting monoenergy photon with 662 keV for the comparison of the scintillation efficiency. As a result, it was found out that the scintillator sample with 1.00 g of PPO (2,5-Diphenyloxazole) and 0.50 g of POPOP (1,4-Bis(5-phnyl-2oxidazolyl)benzene) dissolved in 100 g of styrene solution has the optimum ratio in terms of the light yield of the polystyrene based plastic scintillator.

The influence of additional fluors on the retention ability of dough and the technological quality of bakery products

Directory of Open Access Journals (Sweden)

Tatiana Bojňanská

2015-08-01

Full Text Available The work monitored rheofermentation properties of dough prepared from composite flours formed by 70% of wheat flour T650 and the addition of 30%. Three kinds of additions were used, namely spelt flour, amaranth flour and buckwheat flour. To determine rheofermentation properties Rheofermentometer Rhea F4 was used, by means of which the dough development, the production of fermentation gases, retention ability of dough and the activity of used baking yeast were analysed. The best ability to retain formed fermentation gas had wheat flour (control and composite flour with the addition of spelt flour. The composite flour with addition of amaranth flour showed a retention coefficient compared to the control lower by 13%, and the composite flour with addition of buckwheat flour showed a retention coefficient compared to the control reduced by 20%. Control flour and composite flours were then processed in the baking experiment. Based on its results it was possible to evaluate the effect of the addition and retention capacity of dough on the quality of the final products (experimental loaves. The biggest loaf volume (200 cm3 and the optimal vaulting (0.65 were found in the control and a loaf made of composite flour with addition of spelt. Loaf volume, produced from composite flours with the addition of amaranth, and buckwheat was compared to control lower by 18.7%, and 16.3% respectively. The value of vaulting of these products (0.40 can be evaluated as unsatisfactory. Based on the evaluation of results observed by measuring on the rheofermentometer and baking experiment results it can be concluded that a better ability to retain the formed fermentation gas, thus ensuring high volume, had loaves made from wheat flour T650 and composite flour with addition of spelt flour. Based on the findings, it is possible to state that the results of rheofermentometric measurements predict the volume and vaulting of bakery products. By means of Rheofermentometer valuable information has been gained concerning the quality of bakery ingredients, especially flour and yeast.

Saturation of the hydroxyapatite mineral phase using radioactive fluorine; Saturacion de la fase mineral hidroxiapatita utilizando fluor radiactivo

Energy Technology Data Exchange (ETDEWEB)

Flores de la Torre, J.A.; Badillo A, V.E. [Universidad Autonoma de Zacatecas, 98000 Zacatecas (Mexico); Lopez D, F.A. [Unidad PET Ciclotron, Facultad de Medicina, UNAM, 04510 Mexico D.f. (Mexico)]. e-mail: ebadillo@cantera.reduaz.mx

2005-07-01

With the purpose of knowing the Anion exchange capacity (CIA) of the hydroxyapatite mineral phase, marketed by BIO-RAD, becomes necessary to saturate the surface of the mineral with an anion specie that possesses a strong affinity by this solid as it is the case of the fluorine. Moreover it takes advantage that offers the radioactive tracer technique, using the radioactive isotope of the fluorine, {sup 18}F, produced in the cyclotron of the UNAM. This saturation is obtained in terms of the quantity of retained fluorine (mmol/ 100 g) in the synthetic hydroxyapatite in function of the concentration of the solution of NaF that oscillates from 0.7 M up to 0.16 M to fixed values of pH of 9.2. Those results demonstrate that to this fixed pH value the saturation of the surface of the hydroxyapatite is achieved in approximately 30 mmol/ 100 g, using important concentrations of NaF that correspond to 0.14 M from now on. This result demonstrates the high capacity of the solid considered to retain considerable quantities of fluorine even to basic pH values. (Author)

The effect of anchovy substrate application to fluor retention rate on Sprague Dawley rat tooth email (in vivo)

Science.gov (United States)

Zabrina, S.; Puspitawati, R.; Gunawan, H. A.

2017-08-01

Usage of anchovies (Stolephorus insularis), which contain a high fluoride concentration in a CaF2 compound, needs to be examined as a topical fluoridative agent. Aim: To study the effects of an anchovy substrate application, either by chewing or smearing, in increasing fluoride retention of enamel. Fourteen Sprague Dawley rats were divided into five groups: baseline, experimental (feeding and smearing), and negative controls. After 15 days, lower incisor teeth were extracted and fluoride retention on the enamel surface was measured using EDX. Data were analyzed by the independent samples t-test, the Mann-Whitney U test, and the Kruskal-Wallis test. There was a significant increase in fluoride retention on enamel from the experimental groups compared to the negative control group (p < 0.05). Fluoride retention levels of the experimental feeding group (6.823%) were slightly higher than those of the experimental smearing group (6.783%), though the difference was not statistically significant (p < 0.05). Anchovy substrate application, either by chewing or smearing, increases fluoride retention on tooth enamel.

FINAL FRONTIER AT HANFORD TACKLING THE CENTRAL PLATEAU

International Nuclear Information System (INIS)

GERBER MS

2008-01-01

The large land area in the center of the vast Department of Energy (DOE) Hanford Site in southeast Washington State is known as 'the plateau'--aptly named because its surface elevations are 250-300 feet above the groundwater table. By contrast, areas on the 585-square mile Site that border the Columbia River sit just 30-80 feet above the water table. The Central Plateau, which covers an ellipse of approximately 70 square miles, contains Hanford's radiochemical reprocessing areas--the 200 East and 200 West Areas--and includes the most highly radioactive waste and contaminated facilities on the Site. Five 'canyons' where chemical processes were used to separate out plutonium (Pu), 884 identified soil waste sites (including approximately 50 miles of solid waste burial trenches), more than 900 structures, and all of Hanford's liquid waste storage tanks reside in the Central Plateau. (Notes: Canyons is a nickname given by Hanford workers to the chemical reprocessing facilities. The 177, underground waste tanks at Hanford comprise a separate work scope and are not under Fluor's management). Fluor Hanford, a DOE prime cleanup contractor at the Site for the past 12 years, has moved aggressively to investigate Central Plateau waste sites in the last few years, digging more than 500 boreholes, test pits, direct soil 'pushes' or drive points; logging geophysical data sets; and performing electrical-resistivity scans (a non-intrusive technique that maps patterns of sub-surface soil conductivity). The goal is to identify areas of contamination areas in soil and solid waste sites, so that cost-effective and appropriate decisions on remediation can be made. In 2007, Fluor developed a new work plan for DOE that added 238 soil waste-site characterization activities in the Central Plateau during fiscal years (FYs) 2007-2010. This number represents a 50 percent increase over similar work previously done in central Hanford. Work Plans are among the required steps in the Comprehensive

KOMPOSISI KIMIA DAN ORGANOLEPTIK FORMULA NUGGET BERBASIS TEPUNG TEMPE DAN TEPUNG RICEBRAN

Directory of Open Access Journals (Sweden)

Sufiati Bintanah

2014-07-01

is a food which is capable of decreasing the level of trygliceride, total cholesterol, LDL cholesterol as well as rising HDL cholesterol. Ricebran is also food material which can reduce the blood fat level because of its oryzanol, tokoferol and felurat acid contents. This research aimed to select nugget formula based on its physical, chemical and organoleptic characteristics and its antioxidant activity. Research type is descriptive with  formulation treatment of soy bean and ricebran fluor that consisted of 10 formulas. The analysis of chemical composition was performed on raw materials and nugget comprising protein analysis (micro kjedhl, fat (soxhlet, water (oven, carbohydrate (luff schoorl 1, fixed rate of vitamin E level (alfa-tokoferol, analysis of anti bacterial activities using diffusion method of gelatine. Organoleptic test used scoring method. Research indicated that nugget using soy bean fluor method that accounted for 50% and ricebran comprising 50% (formula A7  gained the highest chemical composition which made up 19.5g% protein, 18.33g% fat, 35.59% water, 1.62% ash, 9.57g% crude fiber, 25.41 g% carbohydrate, 148,92 µg/g vitamin E, 197,1 µg/ml antioxidant activity. Organoleptic test result indicated that it was in formula A7 which was the most favoured. Conclusion: soybean flour, which was optimally gained, was fried without oil for 20 minutes and rice bran optimally gained was fried without oil for 20 minutes. The optimal nugget formula was A7 with a ratio of soybean flour and rice bran 50:50 gr. Keywords: Chemical composition, Organoleptic, Nugget Formula, soy bean fluor, soy bean cake, Ricebran

Clinical and patient-reported outcomes of zirconia-based implant fixed dental prostheses: Results of a prospective case series 5 years after implant placement.

Science.gov (United States)

Spies, Benedikt Christopher; Witkowski, Siegbert; Vach, Kirstin; Kohal, Ralf-Joachim

2018-01-01

To evaluate the clinical and patient-reported outcome of all-ceramic zirconia implant supported fixed dental prostheses (FDPs) 5 years after implant installation. Thirteen patients were treated with two terminally placed one-piece zirconia implants for a three-unit FDP each. The FDPs consisted of a CAD/CAM-fabricated zirconia framework over-pressed with a fluor-apatite veneering ceramic and were adhesively cemented. Survival and success were assessed by applying modified US Public Health Service (USPHS) criteria and preparation of Kaplan-Meier (KM) plots. Alpha and Bravo ratings were accepted for success (among others including small area veneer chippings and occlusal roughness), whereas Charlie ratings allowing for intra-oral correction (e.g., polishing) were accepted for survival. Furthermore, patient-reported outcome measures (PROMs) were analyzed with the help of visual analogue scales (VAS). Wilcoxon matched-pairs signed-rank test (USPHS criteria) and linear mixed models (PROMs) were used to evaluate time effects on response variables. All patients were available 61.8 ± 1.1 months after implant installation (53.6 ± 3.1 months after final prosthesis insertion). FDP survival was 100%. Significant incidence of veneer chipping (p = .0096) and occlusal roughness (p = .0019) was observed. Charlie rated extent of both phenomena resulted in a KM success estimate of 38.5% (95% CI: 14.1%-62.8%; seven FDPs with obvious roughness, three of them with extended veneer chipping). Compared with the pre-treatment assessments (30%-81% of satisfaction), all surveys at prosthetic delivery showed significantly improved VAS scores (66%-93%; p ≤ .038), except for speech (p = .341). Concerning function, esthetics and self-esteem, no decrease in satisfaction could be observed until the end of follow-up (90%-96%; p ≥ .057), whereas perception of sense (92%) and speech (95%) increased over time (p ≤ .030). Occurrence of technical complications did not correlate

Fernald restoration: ecologists and engineers integrate restoration and cleanup

Energy Technology Data Exchange (ETDEWEB)

Woods, Eric; Homer, John

2002-07-15

As cleanup workers excavate pits and tear down buildings at the Fernald site in southwest Ohio, site ecologists are working side-by-side to create thriving wetlands and develop the early stages of forest, prairie, and savanna ecosystems to restore natural resources that were impacted by years of site operations. In 1998, the U.S. Department of Energy-Fernald Office (DOE-FN) and its cleanup contractor, Fluor Fernald, Inc., initiated several ecological restoration projects in perimeter areas of the site (e.g., areas not used for or impacted by uranium processing or waste management). The projects are part of Fernald's final land use plan to restore natural resources over 904 acres of the 1,050-acre site. Pete Yerace, the DOE-FN Natural Resource Trustee representative is working with the Fernald Natural Resource Trustees in an oversight role to resolve the state of Ohio's 1986 claim against DOE for injuries to natural resources. Fluor Fernald, Inc., and DOE-FN developed the ''Natural Resource Restoration Plan'', which outlines 15 major restoration projects for the site and will restore injured natural resources at the site. In general, Fernald's plan includes grading to maximize the formation of wetlands or expanded floodplain, amending soil where topsoil has been removed during excavation, and establishing native vegetation throughout the site. Today, with cleanup over 35 percent complete and site closure targeted for 2006, Fernald is entering a new phase of restoration that involves heavily remediated areas. By working closely with engineers and cleanup crews, site ecologists can take advantage of remediation fieldwork (e.g., convert an excavated depression into a wetland) and avoid unnecessary costs and duplication. This collaboration has also created opportunities for relatively simple and inexpensive restoration of areas that were discovered during ongoing remediation. To ensure the survival of the plant material in heavily

Scintillating fiber detector performance, detector geometries, trigger, and electronics issues for scintillating fiber tracking

International Nuclear Information System (INIS)

Baumbaugh, A.E.

1994-06-01

Scintillating Fiber tracking technology has made great advances and has demonstrated great potential for high speed charged particle tracking and triggering. The small detector sizes and fast scintillation fluors available make them very promising for use at high luminosity experiments at today's and tomorrow's colliding and fixed target experiments where high rate capability is essential. This talk will discuss the current state of Scintillating fiber performance and current Visual Light Photon Counter (VLPC) characteristics. The primary topic will be some of the system design and integration issues which should be considered by anyone attempting to design a scintillating fiber tracking system which includes a high speed tracking trigger. Design. constraints placed upon the detector system by the electronics and mechanical sub-systems will be discussed. Seemingly simple and unrelated decisions can have far reaching effects on overall system performance. SDC and DO example system designs will be discussed

Real-time PCR Detection of Brucella Abortus: A Comparative Study of SYBR Green I, 5'-exonuclease, and Hybridization Probe Assays

Energy Technology Data Exchange (ETDEWEB)

Newby, Deborah Trishelle; Hadfield, Ted; Roberto, Francisco Figueroa

2003-08-01

Real-time PCR provides a means of detecting and quantifying DNA targets by monitoring PCR product accumulation during cycling as indicated by increased fluorescence. A number of different approaches can be used to generate the fluorescence signal. Three approaches—SYBR Green I (a double-stranded DNA intercalating dye), 5'-exonuclease (enzymatically released fluors), and hybridization probes (fluorescence resonance energy transfer)—were evaluated for use in a real-time PCR assay to detect Brucella abortus. The three assays utilized the same amplification primers to produce an identical amplicon. This amplicon spans a region of the B. abortus genome that includes portions of the alkB gene and the IS711 insertion element. All three assays were of comparable sensitivity, providing a linear assay over 7 orders of magnitude (from 7.5 ng down to 7.5 fg). However, the greatest specificity was achieved with the hybridization probe assay.

Managing public acceptance for a new enrichment facility

International Nuclear Information System (INIS)

Boyd, M.

1992-01-01

The Claiborne Enrichment Center has many first associated with it, and a solid nuclear-based community relations experience has been combined with fly-by-the-seat-of-your-pants techniques to successfully introduce a first-of-a-kind facility not only to Louisiana, but also to the US. The project is being developed by Louisiana Energy Services (LES), a limited partnership consisting of Urenco, the European enrichment consortium that operates centrifuge enrichment facilities in the United Kingdom, the Netherlands, and Germany; Fluor Daniel, an internationally known engineering and construction firm; and three utilities - Duke Power, Northern States Power, and Louisiana Power and Light (LP and L). Louisiana Energy will build the nation's first privately owned uranium enrichment facility. It will be the first commercial use of centrifuge technology for enrichment in the US. Some of the community relations challenges the project has had and their solutions are described

Hanford Waste Vitrification Project overview and status

International Nuclear Information System (INIS)

Swenson, L.D.; Smets, J.L.

1993-01-01

The Hanford Waste Vitrification Project (HWVP) is being constructed at the US DOE's Hanford Site in Richland, WA. Engineering and design are being accomplished by Fluor Daniel Inc. in Irvine, CA. Technical input is furnished by Westinghouse Hanford Co. and construction management services by UE ampersand C-Catalytic Inc. The HWVP will immobilize high level nuclear waste in a glass matrix for eventual disposal in the federal repository. The HWVP consists of several structures, the major ones being the Vitrification Building, the Canister Storage Building, fan house, sand filter, waste hold tank, pump house, and administration and construction facilities. Construction started in April 1992 with the clearing and grubbing activities that prepared the site for fencing and construction preparation. Several design packages have been released for procurement activities. The most significant package release is for the Canister Storage Building, which will be the first major structure to be constructed

Waste Treatment Plant Liquid Effluent Treatability Evaluation

International Nuclear Information System (INIS)

LUECK, K.J.

2001-01-01

Bechtel National, Inc. (BNI) provided a forecast of the radioactive, dangerous liquid effluents expected to be generated by the Waste Treatment Plant (WTP). The forecast represents the liquid effluents generated from the processing of 25 distinct batches of tank waste through the WTP. The WTP liquid effluents will be stored, treated, and disposed of in the Liquid Effluent Retention Facility (LERF) and the Effluent Treatment Facility (ETF). Fluor Hanford, Inc. (FH) evaluated the treatability of the WTP liquid effluents in the LERFIETF. The evaluation was conducted by comparing the forecast to the LERFIETF treatability envelope, which provides information on the items that determine if a liquid effluent is acceptable for receipt and treatment at the LERFIETF. The WTP liquid effluent forecast is outside the current LERFlETF treatability envelope. There are several concerns that must be addressed before the WTP liquid effluents can be accepted at the LERFIETF

Uranium deposit of Bauzot (Saone et Loire); Le gisement d'uranium de Bauzot (Saone et Loire)

Energy Technology Data Exchange (ETDEWEB)

Carrat, G H [Commissariat a l' Energie Atomique, Saone et Loire (France). Centre d' Etudes Nucleaires

1956-07-01

The best known of the uranium ore deposits of the Morvan (a province of France) is in the form of a bundle of quartz-fluor lodes with pitchblende and B.P.G.C. ore. The pitchblende seems to have been deposited at different time in respect to the formation of the gangue minerals, but generally it is ore of the first-formed. The main concentrations of ore are always in the vicinity of dykes of basic crystalline rocks. (author) [French] Bauzot, le plus connu des gisements d'uranium du Morvan est constitue d'un faisceau de filons quartzofluores, mineralise en pechblende et sulfures B.P.G.S. La pechblende semble s'etre mide en place a des periodes variables par rapport a la gangue mais en general elle constitue un des premiers mineraux deposes. Les principaux amas se situent toujours a proximite de filons de roches lamprophyriques. (auteur)

FluoRAS Sensor - Online organic matter for optimising recirculating aquaculture systems

DEFF Research Database (Denmark)

Hambly, Adam; Stedmon, Colin

2018-01-01

FluorRAS will develop a sensor that can save recycled fish farms 30% per year in water and energy consumption for water treatment, as well as optimize nitrogen removal. The sensor will be developed in a partnership between engineers (Krüger A / S) and researchers (DTU), and the product will be made...... both the maintenance of necessary water quality and water treatment costs. Loss of production due to poor water quality is expensive and can be avoided with correct sensor systems. Accumulation of dissolved organic matter and nutrients in the water reduce the effectiveness of UV treatment, is a source...... of nutrition for opportunistic pathogens, and reduces the effectiveness of the biofilter's removing ammonia. Modern recycling systems are therefore dependent on a network of online sensors that monitor and respond to changes in water quality, but currently there is a need for a sensor to monitor...

Beam structure and transverse emittance studies of high-energy ion beams

International Nuclear Information System (INIS)

Saadatmand, K.; Johnson, K.F.; Schneider, J.D.

1991-01-01

A visual diagnostic technique has been developed to monitor and study ion beam structure shape and size along a transport line. In this technique, a commercially available fluorescent screen is utilized in conjunction with a video camera. This visual representation of the beam structure is digitized and enhanced through use of false-color coding and displayed on a TV monitor for on-line viewing. Digitized information is stored for further off-line processing (e.g., extraction of beam profiles). An optional wire grid placed upstream of the fluor screen adds the capability of transverse emittance (or angular spread) measurement to this technique. This diagnostic allows real-time observation of the beam response to parameter changes (e.g., evolution of the beam structure, shifts in the beam intensity at various spatial locations within the beam perimeter, and shifts in the beam center and position). 3 refs., 5 figs

Gamma-Free Neutron Detector Based upon Lithium Phosphate Nanoparticles

International Nuclear Information System (INIS)

Steven Wallace

2007-01-01

A gamma-free neutron-sensitive scintillator is needed to enhance radiation sensing and detection for nonproliferation applications. Such a scintillator would allow very large detectors to be placed at the perimeter of spent-fuel storage facilities at commercial nuclear power plants, so that any movement of spontaneously emitted neutrons from spent nuclear fuel or weapons grade plutonium would be noted in real-time. This task is to demonstrate that the technology for manufacturing large panels of fluor-doped plastic containing lithium-6 phosphate nanoparticles can be achieved. In order to detect neutrons, the nanoparticles must be sufficiently small so that the plastic remains transparent. In this way, the triton and alpha particles generated by the capture of the neutron will result in a photon burst that can be coupled to a wavelength shifting fiber (WLS) producing an optical signal of about ten nanoseconds duration signaling the presence of a neutron emitting source

A double isotope dilution method for assaying of polycyclic aromatic hydrocarbons in cigarette smoke condensate

International Nuclear Information System (INIS)

Bechtold, W.E.; Chen, B.T.

1988-01-01

This report describes a double isotope dilution method for analysis of the polycyclic aromatic hydrocarbons (PAH) phenanthrene, fluor-anthene, pyrene, and benzo[a]pyrene in cigarette smoke particulates. The first isotope dilution used deuterated analogues of the first three PAH as internal standards. The second isotope dilution, for benzo[a]pyrene, used the tritiated analogue as an internal standard. The PAH were isolated from extracts of cigarette smoke particulates using a two-step procedure based on selective extraction from aqueous dimethyl sulfoxide (DMSO) followed by chromatography on silica gel extraction columns. After isolation, aliquots of the samples were analyzed for phenanthrene, pyrene, and fluoranthene by gas chromatography with mass spectrometric detection (GC/MS). Separate aliquots of the samples were analyzed for benzo[a]pyrene by high pressure liquid chromatography with fluorescence detection followed by liquid scintillation spectrometry. PAH levels from cigarette smoke condensates collected from different exposure modes were compared; no exposure-related differences were found. (author)

HANFORD PLUTONIUM FINISHG PLAN (PFP) COMPLETES PLUTONIUM STABILIZATION KEY SAFETY ISSUES CLOSED

International Nuclear Information System (INIS)

GERBER, M.S.

2004-01-01

A long and intense effort to stabilize and repackage nearly 18 metric tons (MT) of plutonium-bearing leftovers from defense production and nuclear experiments concluded successfully in February, bringing universal congratulations to the Department of Energy's Hanford Site in southeast Washington State. The victorious stabilization and packaging endeavor at the Plutonium Finishing Plant (PFP), managed and operated by prime contractor Fluor Hanford, Inc., finished ahead of all milestones in Hanford's cleanup agreement with regulators, and before deadlines set by the Defense Nuclear Facilities Safety Board (DNFSB), a part of the federal Executive Branch that oversees special nuclear materials. The PFP stabilization and packaging project also completed under budget for its four-year tenure, and has been nominated for a DOE Secretarial Award. It won the Project of the Year Award in the local chapter competition of the Project Management Institute, and is being considered for awards at the regional and national level

Homogeneous immunoassay for the cancer marker alpha-fetoprotein using single wavelength excitation fluorescence cross-correlation spectroscopy and CdSe/ZnS quantum dots and fluorescent dyes as labels

International Nuclear Information System (INIS)

Wang, Jinjie; Liu, Heng; Huang, Xiangyi; Ren, Jicun

2016-01-01

The article describes sensitive and selective homogeneous immunoassays for the liver cancer biomarker alpha-fetoprotein (AFP) in human serum by using single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS). Both competitive and sandwich immunoassay modes were applied, and AFP served as a model analyte. Fluorescent CdSe/ZnS quantum dots (with a 655 nm emission peak) and the fluorophore Alexa Fluor 488 (520 nm emission) were chosen to label the antibodies in the sandwich mode, and the antibody and the antigen in the competitive mode. Under optimized conditions, the sandwich assay has a linear dynamic range that covers the 20 pM to 5.0 nM concentration range. The competitive assay, in turn, extends from 180 pM to 15.0 nM. The respective detection limits are 20 pM and 180 pM. The method was successfully applied to directly determine AFP in (spiked) clinical samples, and results were in good agreement with data obtained via ELISAs. (author)

Three-dimensional imaging analysis of Yersinia ruckeri infected rainbow trout (Oncorhynchus mykiss) gills by optical projection tomography

DEFF Research Database (Denmark)

Otani, Maki; Raida, Martin Kristian

Optical projection tomography (OPT) is a new tool for three-dimensional (3D) imaging of small tissues or embryos, based on multi-angle recording of internal fluorescent signals using intact whole mount tissue or fish. To understand the route of infection, gills of Y. ruckeri infected rainbow trout...... were labeled with fluorescent antibody and visualized in 3D by the OPT scanner. Rainbow trout were infected with Y. ruckeri O1 biotype 1 (1 x 109 cells/ml) for 1 hour at 18 °C, and then transferred to clean water. Three fish were sampled at 12 different time points and fixed in 4% PFA. The gills were...... incubated whole with rabbit anti-Y. ruckeri polyclonal antibody and Alexa Fluor®594 conjugated goat anti-rabbit IgG. After embedding in 1% low melting point agarose, specimens were dehydrated in 100% methanol and cleared in BABB (benzyl alcohol: benzyl benzoate) for OPT scanning. 3D imaging results showed...

Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination

International Nuclear Information System (INIS)

Da Silva, Anabela; Leabad, Mehdi; Driol, Clemence; Bordy, Thomas; Debourdeau, Mathieu; Dinten, Jean-Marc; Peltie, Philippe; Rizo, Philippe

2009-01-01

A small-animal multimodality tomography system dedicated to the coregistration of fluorescence optical signal and x-ray measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility of getting in vivo anatomical and functional information simultaneously. Moreover, anatomical measurements can be used as a regularization factor to achieve more accurate reconstructions of the biodistribution of fluorochromes and to speed up treatment. A dedicated acquisition protocol has been established, and the methodology of the reconstruction of the three-dimensional distribution of the biomarkers under cylindrical geometry consistent with classic computed tomography has been implemented. A phantom study was conducted to evaluate and to fix the parameters for the coregistration. These test experiments were reproduced by considering anesthetized mice that had thin glass tubes containing fluorochromes inserted into their esophagus. The instrument is also used for an in vivo biological study conducted on mice with lung tumors, tagged with near-infrared optical probes (targeting probes such as Transferin-AlexaFluor750)

Radon concentrations in the water of Misasa area (Tottori Pref.)

Energy Technology Data Exchange (ETDEWEB)

Morishima, Hiroshige; Koga, Taeko; Inagaki, Masayo [Kinki Univ., Higashi-Osaka, Osaka (Japan); Mifune, Masaaki

1997-02-01

UNSCEAR (United Nations Scientific Committee on the Effects of Atomic Radiation) Report (1988, 1993) indicated that the internal exposure dose of absorption of radon (Rn-222) and the short-lived decay product nucleus occupied about half of 2.4 m Sv of the global average of annual dose of natural radiation source. It is said to be the largest factor of radiation dose of natural radiation. Sample collection and the method, the elution properties by the concentration of Rn-222 in water and the comparison of characteristics among the different methods are explained. The detection limit of concentration of radon in water by OPTI-FLUOR direct method using the liquid scintillator spectrometer was 1.5 Bq/l for 20 mins counting. The maximum radon concentrations determined by this method showed 1700 Bq/l of the spring water in Misasa area, 400 Bq/l of the small water supply system and well and 18 Bq/l of Mitoku river. (S.Y.)

Hanford Site near-facility environmental monitoring data report for calendar year 1998

Energy Technology Data Exchange (ETDEWEB)

DIEDIKER, L.P.

1999-07-29

This document summarizes the results of the U.S. Department of Energy's Near-Facility Environmental Monitoring program conducted by Waste Management Federal Services of Hanford, Inc. for Fluor Daniel Hanford, Inc. for 1998 in the 100,200/600, and 300/400 Areas of the Hanford Site, in southcentral Washington State. Surveillance activities included sampling and analyses of ambient air, surface water, groundwater, soil, sediments, and biota. Also, external radiation measurements and radiological surveys were taken at waste disposal sites, radiologically controlled areas, and roads. These activities were conducted to assess and control the effects of nuclear facilities and waste sites on the local environment. In addition, diffuse sources were monitored to determine compliance with federal, state, and/or local regulations. In general, although effects from nuclear facilities can still be observed on the Hanford Site and radiation levels are slightly elevated when compared to offsite locations, the differences are less than in previous years.

Preliminary study of light yield dependence on LAB liquid scintillator composition

International Nuclear Information System (INIS)

Ye Xingchen; Yu Boxiang; Zhou Xiang

2015-01-01

Liquid scintillator (LS) will be adopted as the detector material in JUNO (Jiangmen Underground Neutrino Observatory). The energy resolution requirement of JUNO is 3%, which has never previously been reached. To achieve this energy resolution, the light yield of liquid scintillator is an important factor. PPO (the fluor) and bis-MSB (the wavelength shifter) are the two main materials dissolved in LAB. To study the influence of these two materials on the transmission of scintillation photons in LS, 25 and 12 cm-long quartz vessels were used in a light yield experiment. LS samples with different concentration of PPO and bis-MSB were tested. At these lengths, the light yield growth is not obvious when the concentration of PPO is higher than 4 g/L. The influence from bis-MSB becomes insignificant when its concentration is higher than 8 mg/L. This result could provide some useful suggestions for the JUNO LS. (authors)

Dutch offshore skills assessed

Energy Technology Data Exchange (ETDEWEB)

Schaap, P

1988-11-01

The position is described of the different Dutch industries involved in the oil and gas exploitation business in the North Sea. Mentioned are, in the first place, the activities of towing services Smit Tak, and Wijsmuller. A well known drilling contractor is Neddrill. Drilling rigs are often designed in cooperation with consulting engineers firms Protech International, Heerema Engineering, Global Engineering, KTI, Fluor, and Tebodin. Drilling rigs constructors Grootint, Mercon Steel Structures, Heerema Havenbedrijf, HCG, Boele, and IHC have a sound name in the offshore construction scene. Offshore contractors like Heerema, Smit Tak, Volker Stevin and Allseas Engineering should be mentioned too. Special attention is given to the operation of saving drilling rigs in the Ekofisk field. Years of exploitation of oil and gas from this field resulted in subsidence of the sea floor, which endangered the drilling rigs. Hydraudyne designed the hydraulic lifter necessary for this saving operation. Dutch firms in this field have an estimated yearly turnover of Dfl 3 x 10/sup 9/. 6 figs.

Hanford Site near-facility environmental monitoring data report for calendar year 1998

International Nuclear Information System (INIS)

DIEDIKER, L.P.

1999-01-01

This document summarizes the results of the U.S. Department of Energy's Near-Facility Environmental Monitoring program conducted by Waste Management Federal Services of Hanford, Inc. for Fluor Daniel Hanford, Inc. for 1998 in the 100,200/600, and 300/400 Areas of the Hanford Site, in southcentral Washington State. Surveillance activities included sampling and analyses of ambient air, surface water, groundwater, soil, sediments, and biota. Also, external radiation measurements and radiological surveys were taken at waste disposal sites, radiologically controlled areas, and roads. These activities were conducted to assess and control the effects of nuclear facilities and waste sites on the local environment. In addition, diffuse sources were monitored to determine compliance with federal, state, and/or local regulations. In general, although effects from nuclear facilities can still be observed on the Hanford Site and radiation levels are slightly elevated when compared to offsite locations, the differences are less than in previous years

Organelle-Specific Activity-Based Protein Profiling in Living Cells

Energy Technology Data Exchange (ETDEWEB)

Wiedner, Susan D.; Anderson, Lindsey N.; Sadler, Natalie C.; Chrisler, William B.; Kodali, Vamsi K.; Smith, Richard D.; Wright, Aaron T.

2014-02-06

A multimodal acidic organelle targeting activity-based probe was developed for analysis of subcellular native enzymatic activity of cells by fluorescent microscopy and mass spectrometry. A cathepsin reactive warhead was conjugated to an acidotropic amine, and a clickable alkyne for appendage of AlexaFluor 488 or biotin reporter tags. This probe accumulated in punctate vesicles surrounded by LAMP1, a lysosome marker, as observed by Structured Illumination Microscopy (SIM) in J774 mouse macrophage cells. Biotin conjugation, affinity purification, and analysis of in vivo labeled J774 by mass spectrometry showed that the probe was very selective for Cathepsins B and Z, two lysosomal cysteine proteases. Analysis of starvation induced autophagy, which is an increase in cell component catabolism involving lysosomes, showed a large increase in tagged protein number and an increase in cathepsin activity. Organelle targeting activity-based probes and subsequent analysis of resident proteins by mass spectrometry is enabled by tuning the physicochemical properties of the probe.

Dynamic simulation of a direct carbonate fuel cell power plant

Energy Technology Data Exchange (ETDEWEB)

Ernest, J.B. [Fluor Daniel, Inc., Irvine, CA (United States); Ghezel-Ayagh, H.; Kush, A.K. [Fuel Cell Engineering, Danbury, CT (United States)

1996-12-31

Fuel Cell Engineering Corporation (FCE) is commercializing a 2.85 MW Direct carbonate Fuel Cell (DFC) power plant. The commercialization sequence has already progressed through construction and operation of the first commercial-scale DFC power plant on a U.S. electric utility, the 2 MW Santa Clara Demonstration Project (SCDP), and the completion of the early phases of a Commercial Plant design. A 400 kW fuel cell stack Test Facility is being built at Energy Research Corporation (ERC), FCE`s parent company, which will be capable of testing commercial-sized fuel cell stacks in an integrated plant configuration. Fluor Daniel, Inc. provided engineering, procurement, and construction services for SCDP and has jointly developed the Commercial Plant design with FCE, focusing on the balance-of-plant (BOP) equipment outside of the fuel cell modules. This paper provides a brief orientation to the dynamic simulation of a fuel cell power plant and the benefits offered.

Preparation and performance study of a novel liquid scintillator with mixed solvent as the matrix

International Nuclear Information System (INIS)

Zheng, Zhanlong; Zhu, Jiayi; Luo, Xuan; Xu, Yewei; Zhang, Qianfeng; Zhang, Xing; Bi, Yutie; Zhang, Lin

2017-01-01

A novel liquid scintillator with the mixed solvent as the matrix was prepared for obtaining a good comprehensive performance. In this ternary liquid scintillator, the combination of 20% pseudocumene (PC) and 80% linear-alkyl benzene (LAB) by volume was chosen as the mixed solvent, and 2,5-diphenyloxazole (PPO) and 1,4-bis(2-Methylstyryl) benzene (bis-MSB) were as the primary fluor and wavelength shifter, respectively. The optimum prescription was obtained with regard to the light yield. Some characterizations based on the optimal formulation were conducted. The fluorescence emission spectra and wavelength-dependent optical attenuation length of the sample were measured by the fluorescence spectrophotometer and an UV–Vis spectrometer, respectively. The light yield was characterized by adopting the home-made optical platform device. The decay time was tested by adopting the time-correlated single photon counting (TCSPC) technique featured in high dynamic range of several orders of magnitude in light intensity. The experimental test results showed that the sample had a fairly good comprehensive performance.

Synthesis and Characterization of Nanodiamond-Growth Factor Complexes Towards Applications in Oral Implantation and Regenerative Medicine.

Science.gov (United States)

Bang, Julie; Ting, Caleb; Wang, Peter; Kim, Ted; Wang, Kenneth; Kee, Theodore; Miya, Darron; Ho, Dean; Lee, Dong-Keun

2018-02-19

Current challenges in the field of regenerative medicine include the need to deliver sustained concentrations of growth factors and genes that are required to induce the repair of deficient tissues. Enhancement of drug delivery and uptake may result in improved growth factor efficacy. Nanodiamonds (NDs) were explored as potential growth factor delivery agents due to the many favorable properties that they possess. For example, ND's biocompatibility has been extensively validated pre-clinically. In addition, they can be scalably produced through impact events such as detonation. They possess notably faceted surfaces with diverse electrostatic properties that allow the rapid formation of growth factor complexes. In this study, a complex based on NDs conjugated to epidermal growth factor (EGF) functionalized with Alexa Fluor 488 (ND-EGF) was developed. ND-EGF was comprehensively evaluated using dynamic light scattering and zeta potential analysis. Furthermore, the NDs were capable of eluting EGF in a sustained fashion. Therefore, ND-EGF may serve as a promising nano-biomaterial for sustained growth factor elution.

Preparation and performance study of a novel liquid scintillator with mixed solvent as the matrix

Energy Technology Data Exchange (ETDEWEB)

Zheng, Zhanlong [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621010 (China); Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology and Research Center of Laser Fusion, Mianyang 621010 (China); Zhu, Jiayi [Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology and Research Center of Laser Fusion, Mianyang 621010 (China); Luo, Xuan, E-mail: luox76@gmail.com [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Xu, Yewei [School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621010 (China); Zhang, Qianfeng [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology and Research Center of Laser Fusion, Mianyang 621010 (China); Zhang, Xing [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); Bi, Yutie, E-mail: biyutie@sina.com [Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology and Research Center of Laser Fusion, Mianyang 621010 (China); Zhang, Lin [Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang 621900 (China); School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621010 (China); Joint Laboratory for Extreme Conditions Matter Properties, Southwest University of Science and Technology and Research Center of Laser Fusion, Mianyang 621010 (China)

2017-04-01

A novel liquid scintillator with the mixed solvent as the matrix was prepared for obtaining a good comprehensive performance. In this ternary liquid scintillator, the combination of 20% pseudocumene (PC) and 80% linear-alkyl benzene (LAB) by volume was chosen as the mixed solvent, and 2,5-diphenyloxazole (PPO) and 1,4-bis(2-Methylstyryl) benzene (bis-MSB) were as the primary fluor and wavelength shifter, respectively. The optimum prescription was obtained with regard to the light yield. Some characterizations based on the optimal formulation were conducted. The fluorescence emission spectra and wavelength-dependent optical attenuation length of the sample were measured by the fluorescence spectrophotometer and an UV–Vis spectrometer, respectively. The light yield was characterized by adopting the home-made optical platform device. The decay time was tested by adopting the time-correlated single photon counting (TCSPC) technique featured in high dynamic range of several orders of magnitude in light intensity. The experimental test results showed that the sample had a fairly good comprehensive performance.

Evaluation of Fluorine-18 as a Scanning Agent for Intracranial Tumours; Emploi du Fluor-18 en Scintigraphy des Tumeurs Intracraniennes; Otsenka Ftora-18 kak skenniruyushchego agenta dlya vnutricherepnykh opukholej; Examen de las Caracteristicas del Fluor-18 como Agente de Exploracion de los Tumores Intracraneales

Energy Technology Data Exchange (ETDEWEB)

Entzian, W.; Aronow, S.; Soloway, A. H.; Sweet, W. H. [Massachusetts General Hospital, Boston, MA (United States)

1964-10-15

F{sup 18}, a 110-min half-life, positron emitter was studied as a possible agent for brain-tumour localization. It was prepared in a nuclear reactor by thermal neutron irradiation of Li{sub 2}CO{sub 3} (Li{sup 6} (n, {alpha}) H{sup 3}, O{sup 16}(H{sup 3}, n) F{sup 18}). Tissue studies in mice bearing subcutaneously-transplanted ependymomas indicated that labelled sodium fluoride (NaF{sup 18}) gave excessive concentrations in bone and was therefore unsuitable as a brain-scanning agent. However, labelled potassium fluoborate (KBF{sup 18}{sub 4}) showed good preferential uptake in tumour compared with brain, uptake ratios ranging from 8 to 11. No excessive concentrations were found in other organs in either mice or cats. Toxicity studies were carried out in mice and rabbits at levels of 300 mg/kg and 120 mgAg respectively. No adverse effects were observed provided the solution was neutralized to a physiological pH. The chemical amounts needed for patient scanning were less than 0.5 mgAg of body weight. A comparative clinical study of 10 patients, each scanned with KBF{sup 18}{sub 4} and other isotopic agents, corroborated the findings of Askenasy et al. that this is a useful compound for brain-tumour localization. Intravenous injection was optimally performed 30 min before scanning at a dosage of 15 {mu}c/kg of body weight. Tissue samples of tumour and normal brain were obtained from one patient at operation. The uptake ratio was 3.5. While this is lower than that found in the mouse, it is still in a useful range. Blood samples obtained from patients at varying time intervals after injection yielded a blood clearance curve which had two time constants. The first was very rapid and the second slower. This sequence is typical of useful scanning agents. (author) [French] Les auteurs ont etudie la possibilite d'employer {sup 18}F, emetteur de positons ayant une periode de 110 min, pour localiser lcs tumeurs du cerveau. Cette substance a ete preparee dans un reacteur par irradiation de Li{sub 2}Co{sub 3} par des neutrons thermiques ({sup 6}Li (n, {alpha}){sup 3}H, {sup 15}O({sup 3}H, n){sup 18}F). Il ressort d'etudes faites sur des tissus de souris presentant des ependymomes implantes par greffes sous-cutanees que le fluorure de sodium marque (Na{sup 18}F) se concentre de maniere excessive dans les os et qu'il ne convient donc pas pour explorer le cerveau. Par contre, on a constate que le fluoborate de potassium marque (KBF{sup 18}{sub 4}) se concentrait nettement plus dans les tumeurs que dans le tissu normal du cerveau, les rapports de concentration allant de 8 a 11. Aucune concentration excessive n'a ete observee dans d'autres organes, tant chez les souris que chez les chats. Les auteurs ont fait des etudes de toxicite sur des souris et des lapins pour 300 mgAg et 120 mgAg' respectivement. Aucun effet nocif n'a ete observe tant que le pH de la solution etait maintenu a une valeur physiologique. Les quantites de substance necessaire pour proceder a la scintigraphie du sujet etaient inferieures a 0,5 mgAg de poids du corps. Une etude clinique comparative menee sur 10 patients, qui ont tous ete examines a l'aide de KBF{sup 18}{sub 4} et d'autres agents radioisotopiques, a confirme la conclusion d'Askenasy et autres: ce compose est utile pour localiser les tumeurs du cerveau. On a fait des injections intraveineuses dans des conditions optimales 30 min avant la scintigraphie, a raison de 15 {mu}c/kg de poids du corps. Des echantillons de tissus de tumeur et de tissus normaux du cerveau ont ete preleves sur un malade qui subissait une operation. Le rapport de concentration etait de 3,5. Bien que ce rapport soit inferieur a celui qui avait ete observe chez la souris, il n'en demeure pas moins convenable. L'examen d'echantillons de sang preleves sur des malades a divers intervalles apres l'injection a permis d'etablir une courbe d'elimination du sang a deux constantes de temps. La premiere etait tres rapide et la seconde plus lente, ce qui est caracteristique des bons agents de scintigraphie. (author) [Spanish] Los autores estudiaron la posibilidad de utilizar {sup 18}F (periodo: 110 min) para localizar tumores cerebrales. Prepararon este isotopo en un reactor por irradiacion de Li{sub 2}CO{sub 3} con neutrones termicos ({sup 6}Li (n, {alpha}){sup 3} H, {sup 16}O({sup 3}H, n) {sup 18}F). Los estudios realizados con tejidos de raton en el que se habfe transplantado epen- dimomas.por vCa subcutanea mostraron que el fluoruro de sodio marcado (Na{sup 18}F) se concentra excesivamente en los huesos y que, por tanto, no es adecuado para explorar el cerebro. Sin embargo, el tumor capto una proporcion mayor de fluoborato potasico marcado (KB{sup 18}F{sub 4}) que el tejido normal del cerebro; la razon de captacion fue de 8 a 11. En los experimentos efectuados con ratones y gatos no se observo ninguna concentracion excesiva en otros organos. Los autores efectuaron estudios de toxicidad sobre ratones y conejos con dosis de 300 mgAg y 120 mg/kg respectivamente. No se observo ningun efecto adverso cuando la solucion estaba neutralizada hasta alcanzar un pH fisiologico. La cantidad de productos quimicos necesaria para la exploracion fue inferior a 0,5 mg/kg de peso corporal. Un estudio clinico comparativo de 10 pacientes, explorados despues de administrarles KB{sup 18}F{sub 4} y otros agentes isotopicos, corroboro los resultados obtenidos por Askenasy y sus colaboradores, segun los cuales este compuesto es util para localizar tumores cerebrales. La inyeccion intravenosa dio resultados optimos cuando se administraron 15 {mu}c por kg de peso corporal 30 min antes de proceder a la exploracion. Se sacaron muestras de tumor y de tejido cerebral normal de un paciente durante la operacion. La razon de absorcion tumor/tejido normal fue de 3,5. Si bien este valor es inferior al correspondiente al raton, presenta todavia utilidad diagnostica. Las muestras de sangre extraidas a diferentes intervalos despues de la inyeccion permitieron trazar una curva de depuracion sanguinea con dos constantes de tiempo. La primera era muy breve y la segunda mas larga. Esta secuencia es tipica de los agentes de exploracion eficaces. (author) [Russian] V kachestve verojatnogo agenta dlja lokalizacii mozgovyh opuholej izuchalsja ftor-18, izluchatel' pozitronov s periodom poluraspada 110 min. Ftor-18 poluchali v jadernom reaktore putem obluchenija Li{sub 2}CO{sub 3} (Li{sup 6} (n, {alpha}) H{sup 3}, O{sup 16}(H{sup 3}, n) F{sup 18}) teplovymi nejtronami. Tkanevye issledovanija na myshah s peresazhennoj podkozhno jependimomoj pokazalo, chto mechenyj ftorid natrija (NaF{sup 18}) sozdaet izbytochnye koncentracii v kostjah i pojetomu ne udovletvorjaet v kachestve skennirujushhego agenta dlja mozga. Odnako pri primenenii mechenogo ftorborata kalija (KBF{sup 18}{sub 4})obnaruzheno izbiratel'noe pogloshhenie ego opuhol'ju po sravneniju s mozgom, prichem velichina pogloshhenija izmenjalas' v diapazone ot 8 do 11. Kak u myshej, tak i u koshek ne obnaruzheno izbytochnoj koncentracii v drugih organah. Izuchenie toksichnosti provodilos' na myshah i krolikah pri koncentracii 300mg/kg i 120 mg/kg sootvetstvenno. Pri nejtralizacii rastvora do urovnja fiziologicheskogo pH ne nabljudalos' nikakih neblagoprijatnyh jeffektov. Himicheskie kolichestva, neobhodimye dlja skennirovanija pacientov, sostavljali menee 0,5 mg/kg vesa tela. Sravnitel'nye klinicheskie issledovanija u 10 pacientov, kazhdyj iz kotoryh podvergalsja skennirovaniju s KBF{sup 18}{sub 4} drugimi izotopnymi veshhestvami, podtverdili vyvody Ashkenazi i dr. o tom, chto jeto soedinenie javljaetsja poleznym dlja opredelenija lokalizacii mozgovyh opuholej. Vnutrivennoe vvedenie osushhestvljalos' optimal'no za 30 minut do skennirovanija pri doze 15 mkkjuri/kg vesa tela. U odnogo pacienta pri operacii byli vzjaty obrazcy tkanej opuholi i zdorovoj tkani mozga. Kojefficient pogloshhenija sostavljal 3,5. Hotja jeto sootnoshenie nizhe, chem u myshej, no jeta velichina prakticheski prigodna. Pri issledovanii krovi, vzjatoj u pacientov cherez raznye promezhutki vremeni posle in{sup e}kcii, poluchena krivaja klirensa krovi, imejushhaja dve postojannyh vremeni. Pervaja-ochen' kratkovremennaja, vtoraja -medlennee. Takaja harakteristika tipichna dlja prigodnyh sken- nirujushhih agentov. (author)

Facilitated monocyte-macrophage uptake and tissue distribution of superparmagnetic iron-oxide nanoparticles.

Directory of Open Access Journals (Sweden)

Arnaud Beduneau

Full Text Available BACKGROUND: We posit that the same mononuclear phagocytes (MP that serve as target cells and vehicles for a host of microbial infections can be used to improve diagnostics and drug delivery. We also theorize that physical and biological processes such as particle shape, size, coating and opsonization that affect MP clearance of debris and microbes can be harnessed to facilitate uptake of nanoparticles (NP and tissue delivery. METHODS: Monocytes and monocyte-derived macrophages (MDM were used as vehicles of superparamagnetic iron oxide (SPIO NP and immunoglobulin (IgG or albumin coated SPIO for studies of uptake and distribution. IgG coated SPIO was synthesized by covalent linkage and uptake into monocytes and MDM investigated related to size, time, temperature, concentration, and coatings. SPIO and IgG SPIO were infused intravenously into naïve mice. T(2 measures using magnetic resonance imaging (MRI were used to monitor tissue distribution in animals. RESULTS: Oxidation of dextran on the SPIO surface generated reactive aldehyde groups and permitted covalent linkage to amino groups of murine and human IgG and F(ab'(2 fragments and for Alexa Fluor(R 488 hydroxylamine to form a Schiff base. This labile intermediate was immediately reduced with sodium cyanoborohydride in order to stabilize the NP conjugate. Optical density measurements of the oxidized IgG, F(ab'(2, and/or Alexa Fluor(R 488 SPIO demonstrated approximately 50% coupling yield. IgG-SPIO was found stable at 4 degrees C for a period of 1 month during which size and polydispersity index varied little from 175 nm and 200 nm, respectively. In vitro, NP accumulated readily within monocyte and MDM cytoplasm after IgG-SPIO exposure; whereas, the uptake of native SPIO in monocytes and MDM was 10-fold less. No changes in cell viability were noted for the SPIO-containing monocytes and MDM. Cell morphology was not changed as observed by transmission electron microscopy. Compared to unconjugated

The Fernald Closure Project: Lessons Learned

International Nuclear Information System (INIS)

Murphy, Cornelius M.; Carr, Dennis

2008-01-01

For nearly 37 years, the U.S. Department of Energy site at Fernald - near Cincinnati, Ohio - produced 230,000 metric tons (250,000 short tons) of high-purity, low-enriched uranium for the U.S. Defense Program, generating more than 5.4 million metric tons (6 million short tons) of liquid and solid waste as it carried out its Cold War mission. The facility was shut down in 1989 and clean up began in 1992, when Fluor won the contract to clean up the site. Cleaning up Fernald and returning it to the people of Ohio was a $4.4 billion mega environmental-remediation project that was completed in October 2006. Project evolved through four phases: - Conducting remedial-investigation studies to determine the extent of damage to the environment and groundwater at, and adjacent to, the production facilities; - Selecting cleanup criteria - final end states that had to be met that protect human health and the environment; - Selecting and implementing the remedial actions to meet the cleanup goals; - Executing the work in a safe, compliant and cost-effective manner. In the early stages of the project, there were strained relationships - in fact total distrust - between the local community and the DOE as a result of aquifer contamination and potential health effects to the workers and local residents. To engage citizens and interested stakeholders groups in the decision-making process, the DOE and Fluor developed a public-participation strategy to open the channels of communication with the various parties: site leadership, technical staff and regulators. This approach proved invaluable to the success of the project, which has become a model for future environmental remediation projects. This paper will summarize the history and shares lessons learned: the completion of the uranium-production mission to the implementation of the Records of Decision defining the cleanup standards and the remedies achieved. Lessons learned fall into ten categories: - Regulatory approach with end

Human eyelid meibomian glands and tarsal muscle are recognized by autoantibodies from patients affected by a new variant of endemic pemphigus foliaceus in El-Bagre, Colombia, South America.

Science.gov (United States)

Abreu-Velez, Ana Maria; Howard, Michael S; Hashimoto, Takashi; Grossniklaus, Hans E

2010-03-01

Previously, we described a new variant of endemic pemphigus foliaceus (EPF) in Colombia, South America (El Bagre-EPF). Continuing our characterization of this variant of EPF, we now focus on one of our previously reported clinical findings: the presence of ocular lesions. These ocular lesions are seen in patients having extensive skin involvement, as measured by the Lund and Browder scale, which is generally used for patients with skin burns. We specifically searched for evidence of autoreactivity to various eyelid structures in these patients and correlated our immunologic data with the clinical findings. We performed indirect immunofluorescence studies using normal-appearing human eyelid skin from routine blepharoplasties as substrate tissue. We tested sera from 12 patients with El Bagre-EPF and ocular lesions, 5 patients with sporadic (nonendemic) pemphigus foliaceus, and 20 healthy control subjects (10 from the El Bagre-EPF endemic area and 10 from nonendemic areas). We used fluorescein isothiocyanate conjugated goat antiserum to human total IgG/IgA/IgM as a secondary antibody. In addition, we used fluorescein isothiocyanate conjugated antibodies to human fibrinogen, albumin, IgG, IgE, C1q, and C3, Texas Red (Rockland Immunochemicals, Inc, Gilbertsville, PA), Alexa Fluor 555, or Alexa Fluor 594 (Invitrogen, Carlsbad, CA). Ki-67 (a cell proliferation marker) was used to determine the cell proliferation rate, and nuclear counterstaining was performed with either 4', 6-diamidino-2-phenylindole or Topro III (Invitrogen, Carlsbad, CA). We observed autoreactivity to multiple eyelid structures, including meibomian glands and tarsal muscle bundles at different levels, and some areas of the epidermis and the dermis close to the isthmus of the eyelids. Tarsal plate autoreactivity was seen in 10 of 12 of the El Bagre-EPF sera and in one control with pemphigus erythematosus. Furthermore, immunoprecipitation using an eyelid sample as a substrate with 1 mmol/L of sodium

Avaliação da cariogenicidade de diferentes tipos de leite e efeitos da suplementação com fluor

OpenAIRE

Luciane Cristina Coppi

2000-01-01

Resumo: Este trabalho teve como objetivos avaliar a cariogenicidade dos leites bovino, humano, formulados infantis e o efeito anticariogênico do flúor, quando suplementado em uma fórmula infantil, utilizando modelo animal de cárie sujeito a um alto desafio cariogênico. Dois experimentos foram realizados para atingir os objetivos propostos. No primeiro experimento, foram utilizadas sessenta ratas Wistar spf (specífíc pathogen free) infectadas com Streptococcus sobrínus e dessalivadas, que rece...

Fluor Hanford Integrated Safety Management System Phase 1 Verification 04/12/2000 Thru 04/28/2000 Volume 1 and 2

International Nuclear Information System (INIS)

PARSONS, J.E.

2000-01-01

The U.S. Department of Energy (DOE) commits to accomplishing its mission safely. To ensure this objective is met, DOE issued DOE P 450.4, Safety Management System Policy, and incorporated safety management into the DOE Acquisition Regulations ([DEAR] 48 CFR 970.5204-2 and 90.5204-78)

Fluor Hanford Integrated Safety Management System Phase 1 Verification 04/12/2000 Thru 04/28/2000 Volume 1 and 2

Energy Technology Data Exchange (ETDEWEB)

PARSONS, J.E.

2000-03-01

The U.S. Department of Energy (DOE) commits to accomplishing its mission safely. To ensure this objective is met, DOE issued DOE P 450.4, Safety Management System Policy, and incorporated safety management into the DOE Acquisition Regulations ([DEAR] 48 CFR 970.5204-2 and 90.5204-78).

Fluor Hanford Integrated Safety Management System Phase 1 Verification 04/12/2000 Thru 04/28/2000 Volume 1 and 2

CERN Document Server

Parsons, J E

2000-01-01

The U.S. Department of Energy (DOE) commits to accomplishing its mission safely. To ensure this objective is met, DOE issued DOE P 450.4, Safety Management System Policy, and incorporated safety management into the DOE Acquisition Regulations ([DEAR] 48 CFR 970.5204-2 and 90.5204-78).

MONA, LISA and VINCI Soon Ready to Travel to Paranal

Science.gov (United States)

2000-11-01

of this very complex system. Subsequently, it will serve to tune this key research facility to the highest possible performance. The VLTI is based on the combination of light (beams) from the telescopes at Paranal. Of these, the four 8.2-m Unit Telescopes are already in operation - they will soon be joined by three 1.8-m telescopes that can be relocated on rails, cf. PR Photo 43b/99. By means of a system of mirrors, the light from two or more of these telescopes will be guided to the central Interferometric Laboratory , at the center of the observing platform on Paranal. Information about the heart of this complex system, the Delay Lines that are located in the underground Interferometric Tunnel, is available with the recent ESO PR Photos 26a-e/00. The VLTI will later receive other front-line instruments, e.g. AMBER , MIDI and PRIMA. When fully ready some years from now, the VLTI will produce extremely sharp images. This will have a major impact on different types of exciting astronomical observations, e.g.: * the direct discovery and imaging of extra-solar planets comparable to Jupiter, * the discovery and imaging of low-mass stars such as brown dwarfs, * observations of star-forming regions and to better understand the physical processes that give birth to stars, * spectral analysis of the atmospheres of nearby stars, and * imaging the objects of the very core of our Galaxy and the detection of black holes in active nuclei of galaxies. The VINCI test instrument The new instrument, VINCI , will soon be delivered to Paranal by the Département de Recherche Spatiale (Department for Space Research), a joint unit of the Centre Nationale de la Recherche Scientifique (French National Centre for Scientific Research) and the Paris Observatory. VINCI is a functional copy of the FLUOR instrument - now at the IOTA (Infrared Optical Telescope Array) interferometer - that has been upgraded and adapted to the needs of the VLTI. FLUOR was developed by the Département de Recherche

Fiscal year 1997-1998 waste information requirements document

International Nuclear Information System (INIS)

Poppiti, J.A.

1997-01-01

The Waste Information Requirements Document describes the activities of the Tank Waste Remediation System (TWRS) Characterization Project that provide characterization information on Hanford Site waste tanks. The characterization information is required to perform operations and meet the commitments of TWRS end users. These commitments are derived from the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement; the Recommendation 93-5 Implementation Plan to the Defense Nuclear Facilities Safety Board (DNFSB); and other directives as listed in Section 4.0. This Waste Information Requirement Document applies to Fiscal Years 1997 and 1998 activities. Its contents are based on the best information available in August 1997. The format and content are based on the directions of DOE-RL (Sieracki, 1997) and Fluor Daniel Hanford Incorporated (Umek, 1997). Activities, such as the revision of the Tank Characterization Technical Sampling Basis (Brown et al. 1997), the revision of the data quality objectives (DQOs), issue closures, discussions with Ecology, and management decisions may cause subsequent updates to the Waste Information Requirements Document

A nanofluidic bioarray chip for fast and high-throughput detection of antibodies in biological fluids

Science.gov (United States)

Lee, Jonathan; Gulzar, Naveed; Scott, Jamie K.; Li, Paul C. H.

2012-10-01

Immunoassays have become a standard in secretome analysis in clinical and research analysis. In this field there is a need for a high throughput method that uses low sample volumes. Microfluidics and nanofluidics have been developed for this purpose. Our lab has developed a nanofluidic bioarray (NBA) chip with the goal being a high throughput system that assays low sample volumes against multiple probes. A combination of horizontal and vertical channels are produced to create an array antigens on the surface of the NBA chip in one dimension that is probed by flowing in the other dimension antibodies from biological fluids. We have tested the NBA chip by immobilizing streptavidin and then biotinylated peptide to detect the presence of a mouse monoclonal antibody (MAb) that is specific for the peptide. Bound antibody is detected by an AlexaFluor 647 labeled goat (anti-mouse IgG) polyclonal antibody. Using the NBA chip, we have successfully detected peptide binding by small-volume (0.5 μl) samples containing 50 attomoles (100 pM) MAb.

Copper is an endogenous modulator of neural circuit spontaneous activity.

Science.gov (United States)

Dodani, Sheel C; Firl, Alana; Chan, Jefferson; Nam, Christine I; Aron, Allegra T; Onak, Carl S; Ramos-Torres, Karla M; Paek, Jaeho; Webster, Corey M; Feller, Marla B; Chang, Christopher J

2014-11-18

For reasons that remain insufficiently understood, the brain requires among the highest levels of metals in the body for normal function. The traditional paradigm for this organ and others is that fluxes of alkali and alkaline earth metals are required for signaling, but transition metals are maintained in static, tightly bound reservoirs for metabolism and protection against oxidative stress. Here we show that copper is an endogenous modulator of spontaneous activity, a property of functional neural circuitry. Using Copper Fluor-3 (CF3), a new fluorescent Cu(+) sensor for one- and two-photon imaging, we show that neurons and neural tissue maintain basal stores of loosely bound copper that can be attenuated by chelation, which define a labile copper pool. Targeted disruption of these labile copper stores by acute chelation or genetic knockdown of the CTR1 (copper transporter 1) copper channel alters the spatiotemporal properties of spontaneous activity in developing hippocampal and retinal circuits. The data identify an essential role for copper neuronal function and suggest broader contributions of this transition metal to cell signaling.

Simultaneous correlative scanning electron and high-NA fluorescence microscopy.

Directory of Open Access Journals (Sweden)

Nalan Liv

Full Text Available Correlative light and electron microscopy (CLEM is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving regions of interest in both modalities and/or compromises in integrated approaches. We present a novel approach to correlative microscopy, in which a high numerical aperture epi-fluorescence microscope and a scanning electron microscope illuminate the same area of a sample at the same time. This removes the need for retrieval of regions of interest leading to a drastic reduction of inspection times and the possibility for quantitative investigations of large areas and datasets with correlative microscopy. We demonstrate Simultaneous CLEM (SCLEM analyzing cell-cell connections and membrane protrusions in whole uncoated colon adenocarcinoma cell line cells stained for actin and cortactin with AlexaFluor488. SCLEM imaging of coverglass-mounted tissue sections with both electron-dense and fluorescence staining is also shown.

AMEC GEOMATRIX/ARA GROUNDWATER REMEDIAITON TRIP REPORT

Energy Technology Data Exchange (ETDEWEB)

SIMMONS SA

2008-08-07

City of Rialto, Well No.3 Demonstration System Integration Project, and Baldwin Park Operable Unit, Baldwin Park, California. The groundwater remediation contractors are AMEC Geomatrix and ARA. The sites were visited on July 22, 2008. Fluor Hanford and the U.S. Department of Energy are currently looking at a variety of alternatives to capture carbon tetrachloride, nitrates, and other COCs from 200-ZP-l groundwater. A few of the more important objectives of our visits were to: (1) Evaluate the treatment systems being used by AMEC Geomatrix to address VOCs, perchlorate, NDMA, 1,4,-Dioxane, and 1,2,3 TCP in a drinking water source; (2) Evaluate how effective these treatment methods have been; (3) Determine the types of problems they have encountered with these treatment systems and how they addressed these problems; (4) Determine the types of secondary wastes being generated by the system; (5) Determine how clean of an operation these companies run; and (6) Determine if the site is worth being visited by DOE-RL at a later date.

An Automated Platform for Assessment of Congenital and Drug-Induced Arrhythmia with hiPSC-Derived Cardiomyocytes

Directory of Open Access Journals (Sweden)

Wesley L. McKeithan

2017-10-01

Full Text Available The ability to produce unlimited numbers of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs harboring disease and patient-specific gene variants creates a new paradigm for modeling congenital heart diseases (CHDs and predicting proarrhythmic liabilities of drug candidates. However, a major roadblock to implementing hiPSC-CM technology in drug discovery is that conventional methods for monitoring action potential (AP kinetics and arrhythmia phenotypes in vitro have been too costly or technically challenging to execute in high throughput. Herein, we describe the first large-scale, fully automated and statistically robust analysis of AP kinetics and drug-induced proarrhythmia in hiPSC-CMs. The platform combines the optical recording of a small molecule fluorescent voltage sensing probe (VoltageFluor2.1.Cl, an automated high throughput microscope and automated image analysis to rapidly generate physiological measurements of cardiomyocytes (CMs. The technique can be readily adapted on any high content imager to study hiPSC-CM physiology and predict the proarrhythmic effects of drug candidates.

Correlative Light-Electron Microscopy of Lipid-Encapsulated Fluorescent Nanodiamonds for Nanometric Localization of Cell Surface Antigens.

Science.gov (United States)

Hsieh, Feng-Jen; Chen, Yen-Wei; Huang, Yao-Kuan; Lee, Hsien-Ming; Lin, Chun-Hung; Chang, Huan-Cheng

2018-02-06

Containing an ensemble of nitrogen-vacancy centers in crystal matrices, fluorescent nanodiamonds (FNDs) are a new type of photostable markers that have found wide applications in light microscopy. The nanomaterial also has a dense carbon core, making it visible to electron microscopy. Here, we show that FNDs encapsulated in biotinylated lipids (bLs) are useful for subdiffraction imaging of antigens on cell surface with correlative light-electron microscopy (CLEM). The lipid encapsulation enables not only good dispersion of the particles in biological buffers but also high specific labeling of live cells. By employing the bL-encapsulated FNDs to target CD44 on HeLa cell surface through biotin-mediated immunostaining, we obtained the spatial distribution of these antigens by CLEM with a localization accuracy of ∼50 nm in routine operations. A comparative study with dual-color imaging, in which CD44 was labeled with FND and MICA/MICB was labeled with Alexa Fluor 488, demonstrated the superior performance of FNDs as fluorescent fiducial markers for CLEM of cell surface antigens.

Environmental Management Performance Report June 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-06-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-RL) a monthly summary of the Project Hanford Management Contractor's (PHMC) Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. In addition to project-specific information, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual Project (e.g., Waste Management, Spent Nuclear Fuels, etc.), in support of Section A of the report. Unless otherwise noted, the Safety, Conduct of Operations, and Cost/Schedule data contained herein is as of April 30, 2000. All other information is updated as of May 19, unless otherwise noted.

Ground state properties of neutron-rich Mg isotopes the "island of inversion" studied with laser and $\\beta$-NMR spectroscopy

CERN Document Server

Kowalska, M

2006-01-01

Studies in regions of the nuclear chart in which the model predictions of properties of nuclei fail can bring a better understanding of the strong interaction in the nuclear medium. To such regions belongs the so called "island of inversion" centered around Ne, Na and Mg isotopes with 20 neutrons in which unexpected ground-state spins, large deformations and dense low-energy spectra appear. This is a strong argument that the magic N=20 is not a closed shell in this area. In this thesis investigations of isotope shifts of stable $^{24-26}$Mg, as well as spins and magnetic moments of short-lived $^{29,31}$Mg are presented. The successful studies were performed at the ISOLDE facility at CERN using collinear laser and $\\beta$-NMR spectroscopy techniques. The isotopes were investigated as single-charged ions in the 280 nm transition from the atomic ground state $^2\\!$S$_{1/2}$ to one of the two lowest excited states $^2\\!$P$_{1/2 ,\\,3/2}$ using continuous wave laser beams. The isotope-shift measurements with fluor...

Micronized Coal Reburning Demonstration for NOx Control: A DOE Assessment

Energy Technology Data Exchange (ETDEWEB)

National Energy Technology Laboratory

2001-08-15

The goal of the U.S. Department of Energy's (DOE) Clean Coal Technology (CCT) program is to furnish the energy marketplace with a number of advanced, more efficient, and environmentally responsible coal utilization technologies through demonstration projects. These projects seek to establish the commercial feasibility of the most promising advanced coal technologies that have developed beyond the proof-of-concept stage. This document serves as a DOE post-project assessment of a project selected in CCT Round IV, the Micronized Coal Reburning (MCR) Demonstration for NO{sub x} Control, as described in a report to Congress (U.S. Department of Energy 1999). The need to meet strict emissions requirements at a minimum cost prompted the Tennessee Valley Authority (TVA), in conjunction with Fuller Company, Energy and Environmental Research Corporation (EER), and Fluor Daniel, to submit the proposal for this project to be sited at TVA's Shawnee Fossil Plant. In July 1992, TVA entered into a cooperative agreement with DOE to conduct the study. However, because of operational and environmental compliance strategy changes, the Shawnee site became unavailable.

An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4.

Science.gov (United States)

He, Hua; Wang, Xiaojuan; Cheng, Tiantian; Xia, Yongqing; Lao, Jun; Ge, Baosheng; Ren, Hao; Khan, Naseer Ullah; Huang, Fang

2016-04-18

Revealing chemokine receptor CXCR4 expression, distribution, and internalization levels in different cancers helps to evaluate cancer progression or prognosis and to set personalized treatment strategy. We here describe a sensitive and high-throughput immunoassay for determining CXCR4 expression and distribution in cancer cells. The assay is accessible to a wide range of users in an ordinary lab only by dip-coating poly(styrene-co-N-isopropylacrylamide) spheres on the glass substrate. The self- assembled spheres form three-dimensional photonic colloidal crystals which enhance the fluorescence of CF647 and Alexa Fluor 647 by a factor of up to 1000. CXCR4 in cells is detected by using the sandwich immunoassay, where the primary antibody recognizes CXCR4 and the secondary antibody is labeled with CF647. With the newly established assay, we quantified the total expression of CXCR4, its distribution on the cell membrane and cytoplasm, and revealed their internalization level upon SDF-1α activation in various cancer cells, even for those with extremely low expression level. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effect of four dental varnishes on the colonization of cariogenic bacteria on exposed sound root surfaces.

Science.gov (United States)

Ekenbäck, S B; Linder, L E; Lönnies, H

2000-01-01

The aim of this study was to evaluate the effect of four different dental varnishes on the colonization of mutans streptococci, total streptococci and lactobacilli on exposed sound root surfaces. Sixty-five individuals were randomly allotted to one of four groups for treatment with Cervitec((R) ) varnish containing 1% chlorhexidine and 1% thymol, a thymol varnish or one of two different fluoride varnishes, Fluor Protector and Duraphat. The varnish was applied to three buccal root surfaces in each patient at baseline and after 1 week. Dental plaque from the root surfaces was collected and analysed on four different occasions: at baseline, after 1 week, 1 month and 6 months. The Cervitec varnish caused a statistically significant reduction in the number of mutans streptococci over time. The reduction was significant at 1 week and 1 month relative to baseline. The numbers of total streptococci and lactobacilli were not significantly affected by treatment with Cervitec. No statistically significant difference over time was found for mutans streptococci, lactobacilli or total streptococci after treatment with the fluoride varnishes or the thymol varnish.

On-line gross alpha radiation monitoring of natural waters with extractive scintillating resins

International Nuclear Information System (INIS)

Hughes, Lara; De Vol, T.A.

2003-01-01

Extractive scintillating resins, which are used to simultaneously separate and quantify radioactivity in aqueous solutions, were developed for low-level alpha radiation monitoring of natural waters. Resins were investigated with bis(2-ethylhexyl)methane-diphosphonic acid (H 2 DEH[MDP], Dipex[reg]) extractant, which has a strong affinity for tri-, tetra- and hexavalent actinides in dilute acids. Extractive scintillating resins were manifested (1) as a mixed bed of scintillating resin and extraction chromatographic resin and (2) by diffusing the organic fluor 2-(1-naphtyl)-5-phenyloxazole into macroporous polystyrene chromatographic resin, then coating with H 2 DEH[MDP], or by coating H 2 DEH[MDP] on scintillating polyvinyltoluene beads. The scintillation light was detected with a modified Hidex Triathler to allow for continuous flow measurements. The average detection efficiencies were 51.7±2.6% and 65.8±10.1% for natural uranium and 241 Am, respectively, for the extractant coated scintillator. The resin was stable for solution flow of up to 1000 ml resulting in rapid real-time quantification of natural uranium in groundwater down to 30 μg/ml

Methacrylate based cross-linkers for improved thermomechanical properties and retention of radiation detection response in plastic scintillators

Science.gov (United States)

Mahl, Adam; Lim, Allison; Latta, Joseph; Yemam, Henok A.; Greife, Uwe; Sellinger, Alan

2018-03-01

Pulse shape discrimination (PSD) is an important method that can efficiently sort and separate neutron and gamma radiation signals. PSD is currently achieved in plastic scintillators by over-doping poly(vinyl toluene) (PVT) matrices with fluorescent molecules. Meaningful separation of the signals requires addition of >20 wt% 2,5-diphenyloxazole (PPO) fluor in PVT. At these concentrations PPO acts as a plasticizer, negatively affecting the physical properties of the final plastic such as hardness, machinability, and thermomechanical stability. This work addresses these issues by implementing a cost-effective solution using cross-linking chemistry via commercially available bisphenol A dimethacrylate (BPA-DM), and a synthesized fluorinated analogue. Both improve the physical properties of over-doped PPO based plastic scintillators without degrading the measured light yield or PSD and Figure of Merit (FoM). In addition, the fluorinated analogue appears to enhance the hydrophobicity of the surface of the plastic scintillators, which may improve the scintillators' resistance to water diffusion and subsequent radiation response degradation. The new formulations improve the feasibility of widely deploying long lifetime PSD capable plastic scintillators in large area coverage assemblies.

Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.

Science.gov (United States)

Hall, Gordon H; Glerum, D Moira; Backhouse, Christopher J

2016-02-01

Electrophoretic separation of fluorescently end-labeled DNA after a PCR serves as a gold standard in genetic diagnostics. Because of their size and cost, instruments for this type of analysis have had limited market uptake, particularly for point-of-care applications. This might be changed through a higher level of system integration and lower instrument costs that can be realized through the use of LEDs for excitation and photodiodes for detection--if they provide sufficient sensitivity. Here, we demonstrate an optimized microchip electrophoresis instrument using polymeric fluidic chips with fluorescence detection of end-labeled DNA with a LOD of 0.15 nM of Alexa Fluor 532. This represents orders of magnitude improvement over previously reported instruments of this type. We demonstrate the system with an electrophoretic separation of two PCR products and their respective primers. We believe that this is the first LED-induced fluorescence microchip electrophoresis system with photodiode-based detection that could be used for standard applications of PCR and electrophoresis. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Interim Report: 100-NR-2 Apatite Treatability Test: Low Concentration Calcium Citrate-Phosphate Solution Injection for In Situ Strontium-90 Immobilization

Energy Technology Data Exchange (ETDEWEB)

Williams, Mark D.; Fritz, Brad G.; Mendoza, Donaldo P.; Rockhold, Mark L.; Thorne, Paul D.; Xie, YuLong; Bjornstad, Bruce N.; Mackley, Rob D.; Newcomer, Darrell R.; Szecsody, James E.; Vermeul, Vincent R.

2008-07-11

Following an evaluation of potential Sr-90 treatment technologies and their applicability under 100-NR-2 hydrogeologic conditions, U.S. Department of Energy, Fluor Hanford, Inc., Pacific Northwest National Laboratory, and the Washington Department of Ecology agreed that the long-term strategy for groundwater remediation at 100-N Area will include apatite sequestration as the primary treatment, followed by a secondary treatment if necessary (most likely phytoremediation). Since then, the agencies have worked together to agree on which apatite sequestration technology has the greatest chance of reducing Sr-90 flux to the river at a reasonable cost. In July 2005, aqueous injection, (i.e., the introduction of apatite-forming chemicals into the subsurface) was endorsed as the interim remedy and selected for field testing. Studies are in progress to assess the efficacy of in situ apatite formation by aqueous solution injection to address both the vadose zone and the shallow aquifer along the 300 ft of shoreline where Sr-90 concentrations are highest. This report describes the field testing of the shallow aquifer treatment.

Gamma irradiation up to 109 rads of all silica fibers, comparison with polymer clad silica fibers and solid sample between 0.4 and 2.5 micrometers

International Nuclear Information System (INIS)

Boisde, G.; Bonnejean, C.; Perez, J.J.; Neumann, V.; Wurier, B.; Boucher, D.

1983-05-01

The effect of γ radiation (Co 60 ) on optical fibers is studied for investigations to remote measurement in irradiating environments. The spectral characteristics of PCS and AS silica fibers, PCS ''Wet'' and AS ''wet'' and ''superwet'' are compared. An automatic computerized system for measurement ''in situ'' is used between 0,4 AND 1,1 micron. Radiation-induced effects are examined at 840nm on PCS, AS with silicone, AS with epoxy (200 and 400/600μm) up to 5.10 8 rads. Samples of ''dry'', ''wet'', ''superwet'' and ''doped fluor'' silica are studied up to 1,3.10 9 rads between 0,3 and 2,5 μm. The photobleaching, the radiation hardening and the breakeage of PCS and AS-silicone after 10 7 rads are recognized. The spectral characteristics of a band at 610-630nm, a spectral deformation at 945nm for ''wet'' fibers and a peak of the radiation-induced attenuation are observed. The kinetic of the 630nm defect in silica fibers and silica samples is also investigated [fr

Proteomics analysis of the endogenous, constitutive, leaf SUMOylome.

Science.gov (United States)

Colignon, Bertrand; Delaive, Edouard; Dieu, Marc; Demazy, Catherine; Muhovski, Yordan; Wallon, Cindy; Raes, Martine; Mauro, Sergio

2017-01-06

SUMOylation is a post-translational modification which regulates a number of critical biological processes in, for example mammals, yeast and plants. In order to fully understand the functional effects of SUMOylation an essential first step is the identification of endogenous targets for SUMOylation. Here we report the results of using a recently developed proteomic approach based on the use of 3D gels to identify the endogenous SUMO targets in leaves of Solanum tuberosum. By using 3D gels we avoid the problem of co-migration of proteins, which is a major limitation of 2D gels, and we enable the use of the highly sensitive CyDye DIGE fluor saturation dyes. Using this new method we have identified 39 individual proteins as probable SUMO targets in leaves of Solanum tuberosum. The advantages of this method compared with other approaches are discussed, and possible future developments are outlined. The authors have no conflicts of interest to declare. All authors have approved the manuscript and agree with submission to Journal of Proteomics. Copyright © 2016. Published by Elsevier B.V.

A precise, efficient radiometric assay for bacterial growth

International Nuclear Information System (INIS)

Boonkitticharoen, V.; Ehrhardt, C.; Kirchner, P.T.

1984-01-01

The two-compartment radiometric assay for bacterial growth promised major advantages over systems in clinical use, but poor reproducibility and counting efficiency limited its application. In this method, 14-CO/sub 2/ produced by bacterial metabolism of C-14-glucose is trapped and counted on filter paper impregnated with NaOH and fluors. The authors sought to improve assay efficiency and precision through a systematic study of relevant physical and chemical factors. Improvements in efficiency (88% vs. 10%) and in precision (relative S.D. 5% vs. 40%) were produced by a) reversing growth medium and scintillator chambers to permit vigorous agitation, b) increasing NaOH quantity and using a supersaturated PPO solution and c) adding detergent to improve uniformity of NaOH-PPO mixture. Inoculum size, substrate concentration and O/sub 2/ transfer rate affected assay sensitivity but not bacterial growth rate. The authors' assay reliably detects bacterial growth for inocula of 10,000 organisms in 1 hour and for 25 organisms within 4 1/2 hours, thus surpassing other existing clinical and research methods

Performances and stability of a 2.4 ton Gd organic liquid scintillator target for ν-bar e detection

International Nuclear Information System (INIS)

Barabanov, I R; Bezrukov, L B; Danilov, N A; Krilov, Yu S; Yanovich, E A; Malguin, A S; Cattadori, C M; Vacri, A di; Ioannucci, L; Bruno, G; Aglietta, M; Bonardi, A; Fulgione, W; Porta, A; Kemp, E; Selvi, M

2010-01-01

In this paper we report the performance and the chemical and physical properties of a 2 x 1.2 ton organic liquid scintillator target doped with Gd up to ∼ 0.1%, and the results of a 3 year long stability survey of the target. In particular we have measured and monitored the optical and fluorescent properties of the Gd-doped liquid scintillator (LS), the amount of both Gd and primary fluor in solution, and the performance of the two Gd doped targets as neutron detectors, namely neutron capture efficiency and average capture time. The experimental survey is ongoing, the targets being continuously monitored. From the spectrophotometric measurements performed on samples periodically extracted along the three years, we can exclude, at 99% C.L. level, a degradation of the light transmittance of the Gd-doped liquid scintillator larger than 1% y -1 ; from the in-tank measurements no significant decrease of the neutron capture efficiency and neutron capture time is observed. This is the largest stable Gd-doped organic liquid scintillator target ever produced and continuously operated for a long period.

A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.

Science.gov (United States)

Hasan, Md Mehedi; Alam, Mohammad Wajih; Wahid, Khan A; Miah, Sayem; Lukong, Kiven Erique

2016-01-01

This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.

A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability.

Directory of Open Access Journals (Sweden)

Md Mehedi Hasan

Full Text Available This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.

A Low-Cost Digital Microscope with Real-Time Fluorescent Imaging Capability

Science.gov (United States)

Hasan, Md. Mehedi; Wahid, Khan A.; Miah, Sayem; Lukong, Kiven Erique

2016-01-01

This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size. PMID:27977709

New method for GC/FID and GC-C-IRMS analysis of plasma free fatty acid concentration and isotopic enrichment.

Science.gov (United States)

Kangani, Cyrous O; Kelley, David E; Delany, James P

2008-09-15

A simple, direct and accurate method for the determination of concentration and enrichment of free fatty acids (FFAs) in human plasma was developed. The validation and comparison to a conventional method are reported. Three amide derivatives, dimethyl, diethyl and pyrrolidide, were investigated in order to achieve optimal resolution of the individual fatty acids. This method involves the use of dimethylamine/Deoxo-Fluor to derivatize plasma free fatty acids to their dimethylamides. This derivatization method is very mild and efficient, and is selective only towards FFAs so that no separation from a total lipid extract is required. The direct method gave lower concentrations for palmitic acid and stearic acid and increased concentrations for oleic acid and linoleic acid in plasma as compared to methyl ester derivative after thin-layer chromatography. The [(13)C]palmitate isotope enrichment measured using direct method was significantly higher than that observed with the BF(3)/MeOH-TLC method. The present method provided accurate and precise measures of concentration as well as enrichment when analyzed with gas chromatography combustion-isotope ratio-mass spectrometry.

Hanford high-level waste melter system evaluation data packages

International Nuclear Information System (INIS)

Elliott, M.L.; Shafer, P.J.; Lamar, D.A.; Merrill, R.A.; Grunewald, W.; Roth, G.; Tobie, W.

1996-03-01

The Tank Waste Remediation System is selecting a reference melter system for the Hanford High-Level Waste vitrification plant. A melter evaluation was conducted in FY 1994 to narrow down the long list of potential melter technologies to a few for testing. A formal evaluation was performed by a Melter Selection Working Group (MSWG), which met in June and August 1994. At the June meeting, MSWG evaluated 15 technologies and selected six for more thorough evaluation at the Aug. meeting. All 6 were variations of joule-heated or induction-heated melters. Between the June and August meetings, Hanford site staff and consultants compiled data packages for each of the six melter technologies as well as variants of the baseline technologies. Information was solicited from melter candidate vendors to supplement existing information. This document contains the data packages compiled to provide background information to MSWG in support of the evaluation of the six technologies. (A separate evaluation was performed by Fluor Daniel, Inc. to identify balance of plant impacts if a given melter system was selected.)

Flow cytometry as a tool in the evaluation of blood leukocyte function in Chelonia mydas (Linnaeus, 1758 (Testudines, Cheloniidae

Directory of Open Access Journals (Sweden)

S. Rossi

Full Text Available Chelonia mydas is a sea turtle that feeds and nests on the Brazilian coast and a disease called fibropapillomatosis is a threat to this species. Because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. In order to achieve this aim, blood samples were collected from C. mydas with or without fibropapillomas captured on the São Paulo north coast. Blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile RPMI 1640 cell culture medium. Leukocytes were separated by density gradient using Ficoll-PaqueTM Plus, Amershan Biociences®. The following stimuli were applied in the assessment of leukocyte function: Phorbol Miristate-Acetate (PMA for oxidative burst activity evaluation and Zymosan A (Saccharomyces cerevisiae Bio Particles®, Alexa Fluor® 594 conjugate for phagocytosis evaluation. Three cell populations were identified: heterophils, monocytes and lymphocytes. Monocytes were the cells responsible for phagocytosis and oxidative burst.

Applicable safety-related design and operations considerations from the oil and chemical industries

International Nuclear Information System (INIS)

Mulvihill, R.J.; Deshotels, R.L.; Master, C.A.

1987-01-01

Fluor Daniel has conducted several hazards and risk analyses on petroleum and chemical facilities. These analyses included qualitative hazards and operability (HAZOP) studies, preliminary hazards analyses, and qualitative fault-tree analysis as well as quantitative event-tree/fault-tree risk analysis. Several design-related problem areas were uncovered as a result of these analyses as well as deficiencies in operations and maintenance. Design deficiency areas include potential common-mode failures associated with redundant functions sharing a common distributed digital control (DDC) logic circuit board and failures in pressure relief systems. Many of the design weaknesses and potential operator errors discussed have a direct counterpart in nuclear fuel processing plants and nuclear power reactors. Counterparts that are discussed are common cause/common mode failures in control systems and failures in pressure relief systems. Overpressurization of piping and vessels resulting in rupture is discussed. Mitigating design features and operations procedures that have been implemented in the chemical process industry are described and their applicability to the nuclear industry is discussed

ERG [Engineering Review Group] and GRG [Geologic Review Group] review of the horizontal versus vertical modes of waste emplacement at the Deaf Smith County site, Texas

International Nuclear Information System (INIS)

Chytrowski, B.R.

1988-01-01

The Engineering Review Group (ERG) and Geologic Review Group (GRG) were established by the Office of Nuclear Waste Isolation (ONWI) to help evaluate specific issues in the US Department of Energy's nuclear waste repository program. The December 1985 meeting and the February 1986 meeting dealt with the evaluation of the Fluor Technology, Inc., architect-engineer recommendation of the horizontal mode of waste package emplacement for the Site Characterization Plan Conceptual Design Report (SCP-CDR). The ONWI recommendation regarding horizontal and vertical modes of waste package emplacement and associated studies was reviewed. This report documents the ERG and GRG's comments and recommendations on this subject and ONWI responses to the specific points raised by these groups. The ERG and GRG joint review groups concurred with ONWI recommendations that additional studies are required in order to reach a decision on the method of emplacement to be used. In the opinion of these groups, both methods can be implemented; however, should the decision be reached today the vertical mode would be preferred

Gadolinium-loaded gel scintillators for neutron and antineutrino detection

Science.gov (United States)

Riddle, Catherine Lynn; Akers, Douglas William; Demmer, Ricky Lynn; Paviet, Patricia Denise; Drigert, Mark William

2016-11-29

A gadolinium (Gd) loaded scintillation gel (Gd-ScintGel) compound allows for neutron and gamma-ray detection. The unique gel scintillator encompasses some of the best features of both liquid and solid scintillators, yet without many of the disadvantages associated therewith. Preferably, the gel scintillator is a water soluble Gd-DTPA compound and water soluble fluorophores such as: CdSe/ZnS (or ZnS) quantum dot (Q-dot) nanoparticles, coumarin derivatives 7-hydroxy-4-methylcoumarin, 7-hydroxy-4-methylcoumarin-3-acetic acid, 7-hydroxycoumarin-3-carboxylic acid, and Alexa Fluor 350 as well as a carbostyril compound, carbostyril 124 in a stable water-based gel, such as methylcellulose or polyacrylamide polymers. The Gd-loaded ScintGel allows for a homogenious distribution of the Gd-DTPA and the fluorophores, and yields clean fluorescent emission peaks. A moderator, such as deuterium or a water-based clear polymer, can be incorporated in the Gd-ScintGel. The gel scintillators can be used in compact detectors, including neutron and antineutrino detectors.

Micronized Coal Reburning Demonstration for NOx Control: A DOE Assessment; FINAL

International Nuclear Information System (INIS)

National Energy Technology Laboratory

2001-01-01

The goal of the U.S. Department of Energy's (DOE) Clean Coal Technology (CCT) program is to furnish the energy marketplace with a number of advanced, more efficient, and environmentally responsible coal utilization technologies through demonstration projects. These projects seek to establish the commercial feasibility of the most promising advanced coal technologies that have developed beyond the proof-of-concept stage. This document serves as a DOE post-project assessment of a project selected in CCT Round IV, the Micronized Coal Reburning (MCR) Demonstration for NO(sub x) Control, as described in a report to Congress (U.S. Department of Energy 1999). The need to meet strict emissions requirements at a minimum cost prompted the Tennessee Valley Authority (TVA), in conjunction with Fuller Company, Energy and Environmental Research Corporation (EER), and Fluor Daniel, to submit the proposal for this project to be sited at TVA's Shawnee Fossil Plant. In July 1992, TVA entered into a cooperative agreement with DOE to conduct the study. However, because of operational and environmental compliance strategy changes, the Shawnee site became unavailable

On the synthesis of radiofluorinated amino acids by isotope exchange based on the example of 6-[18F]Fluor-L-DOPA

International Nuclear Information System (INIS)

Wagner, F.M.

2008-06-01

In nuclear medical diagnosis, 6-[ 18 F]fluoro-L-3,4-dihydroxyphenylalanine (6-[ 18 F]fluoro-LDOPA), an analogue of L-DOPA, is one of the few established radiopharmaceuticals used for the in vivo investigation of the presynaptic dopaminergic metabolism and of some kind of tumours via Positron Emission Tomography (PET). The presently used method of preparation of the radiotracer by electrophilic labelling is limited to low amounts of activity at high costs. Known nucleophilic syntheses, however, result either in insufficient enantiomeric purity or the known multi-step syntheses are hard to automate, due to their complexity. During this work a novel, easy to automate alternative for the preparation of 6-[ 18 F]fluoro-L-DOPA, was developed and evaluated, using a direct nucleophilic 18 F-fluorination of a protected amino acid derivative. The resulting product has a very high enantiomeric purity. At first, the general suitability of the (S)-BOC-BMI-derivatives for the synthesis of 18 F-labelled amino acids, used in this work, was investigated using a less complex precursor, which resulted in the amino acid 6-[ 18 F]fluoro-L-m-tyrosin via acidic hydrolysis. The preparation of a useful precursor for the nucleophilic 18 F-isotope substitution, namely the (2S,5S)-tert.-butyl- 5-(2-fluoro-5-formylbenzyl)-2-tert. -butyl-3-methyl-4-oxoimidazolidine-1-carbox= yl ate, was investigated in three general different ways. At first it was tried to obtain this product via formylation after coupling with the BOC-BMI, secondly via α,β-dehydro amino acid derivatives and finally via a systematic multi-step synthesis. Only the last mentioned way resulted in a precursor with sufficient purity that could be labelled. The radiochemical yield of the isotopic exchange was about 60 %. In the next step, the presented concept was modified to synthesize a precursor for the preparation of 6-[ 18 F]fluoro-L-DOPA. Only a combination of the protecting groups benzyl and THP resulted in the useful precursor (2S,5S)-tert.-butyl-5-(4-benzyloxy-2-fluoro-5- formylbenzyl)-2-tert.-butyl-3-methyl-4-oxoimidazolidine-1-carboxylate. By optimisation of the isotopic exchange with [ 18 F]fluoride on this precursor a radiochemical yield of about 50 % was achieved. After a three step synthesis including the labelling, a Baeyer-Villiger-oxidation using mCPBA and a hydrolysis using HBr, c.a. 6-[ 18 F]fluoro-L-DOPA was isolated containing more than 99 % of the desired L-isomer. The complete preparation and isolation of c.a. 6-[ 18 F]fluoro-L-DOPA, using optimised conditions, resulted in a decay corrected yield of about 22 % after a synthesis time of 105 minutes. Thus the presented synthesis of 6-[ 18 F]fluoro-L-DOPA is not only more efficient than the known methods of preparation, but is furthermore fully automatable. (orig.)

Dextran sulphate crowding and sodium deoxycholate lysis of primary breast fibroblast cells achieve extracellular matrix deposition and decellularization for breast cancer stem cell culture

Directory of Open Access Journals (Sweden)

Aroem Naruni

2016-01-01

Full Text Available AbstrakLatar belakang: Lingkungan mikro yaitu sel stromal dam matriks ekstraseluler saat ini dinyatakansebagai kontributor dalam perkembangan tumor. Beberapa penelitian telah mengembangkan matriksekstraseluler yang mendukung perkembangan sel in vitro. Matriks ekstraseluler adalah suatu komplekssusunan supramolekuler dari berbagai macam glycoprotein dan proteoglycan. Matriks ekstraselulermenyediakan integritas jaringan, bertindak sebagai scaffold alami tempat sel melekat dan berinteraksiserta berperan sebagai reservoir pertumbuhan sel. Penelitian ini bertujuan untuk mendapatkan deposisidan deselularisasi yang optimal pada matriks ekstraseluler.Metode: Dalam penelitian ini, kami mengembangkan cells crowder untuk meningkatkan deposit matriksekstraseluler dari kultur sel primer fibroblast payudara yang diperoleh dari spesimen hasil operasimammoplasty. Dextran 500 kDa ditambahkan dalam media kultur DMEM lengkap yang telah ditambahkan0.5% FBS dan 100μM L-ascorbic acid 2-phosphate. Setelah tujuh hari, sel dilisis dengan menggunakanSodium Deoxycolate (DOC.Hasil: Deposisi matriks ekstraseluler dan proses deselulerisasi dari sel primer fibroblas payudara dapatterdeteksi dengan menggunakan antibodi Rabbit anti human fibronectin yang selanjutnya ditambahkandengan anti rabbit IgG yang telah dikonjugasi dengan Alexa Fluor 488.Kesimpulan: Penambahan dextran sulfat dan prosesing lysis dengan sodium deoxycolate dapatmeningkatkan deposisi dan menghasilkan deselularisasi matriks ekstraseluler. (Health Science Journalof Indonesia 2015;6:43-7Kata kunci: matriks ekstra selular, kanker mammae, stem cell, sel fibroblast AbstractBackground: The microenvironment including stromal cells and extracellular matrix (ECM is now consideredan active contributor to tumor progression. Certain studies have developed ECM which supports a suitable cellulargrowth in vitro. The ECM is a complex supramolecular assembly of a variety of glycoproteins and proteoglycans

Modification of sulphide catalysts for hydro-treatment by addition of fluorine; Modification de catalyseurs sulfure pour l'hydrotraitement par ajout de fluor

Energy Technology Data Exchange (ETDEWEB)

Fischer, L

1999-12-15

Ni, Mo and NiMo sulphide catalysts supported on alumina were modified with fluorine in the range of 0.8 to 17 weight % F and tested in ortho-xylene hydrogenation under 60 bar total pressure and in presence of 1 bar H{sub 2}S. A positive effect of fluorine on Ni and NiMo catalytic activity was found. The tested catalysts were characterised by electronic microscopy and X-ray-photoelectron spectroscopy. The observed variations in dispersion and sulfidation degree of the active phase are not important enough to explain the good catalytic activity. The catalytic test was adapted in a way that allows a determination of electronic effects on sulphide catalysts under typical hydro-treating conditions. The product distribution in cis- and trans- 1,2-dimethyl-cyclohexane was found to be sensitive to the electronic state of the catalyst's active site. This was verified by addition of electron-donating NH{sub 3} as well as other evidences. According to this test, fluorine acts as an electron-donator on Ni and NiMo catalysts' active sites. A volcano curve of catalytic activity in function of electronic density is obtained for MoS{sub 2} based catalysts (Mo and NiMo), suggesting the existence of an optimum electronic density which would be achieved by introduction of about 6 weight % F into a NiMo catalyst. Characterization by infrared spectroscopy of adsorbed CO confirms the electron-donating effect of fluorine. Fluoridation tests of bulk catalysts permitted to exclude a bonding between fluorine and the active sulphide phase in absence of a support. It is suggested that electron-donating fluoride located on the surface of alumina, the interaction with nickel being of van-der-Waals type. (author)

Synthèse et caractérisation de composés fluorés pour le piégeage de fluorures gazeux

OpenAIRE

Clarenc , Romain Pierre

2010-01-01

This work deals with the synthesis and characterization of alkali, alkali-earth and rare earthbased fluorides and the reactivity of the latter with ReF6 for UF6 purification. In a first part,we focused our attention on the synthesis of KMgF3, MgF2 and CaF2 high surface area metalfluorides coupling both microwave assisted solvothermal process and a fluorination step withelemental fluorine (F2). The higher the surface area, the higher the oxygen rate. Thesenanofluorides exhibit Lewis basic char...

Сomparative Analysis of 0.266 and 0.355 µm Fluorescence Excitation Wavelengths for Laser Fluores-Cence Monitoring of Oil Pollution Detection

Directory of Open Access Journals (Sweden)

M. L. Belov

2017-01-01

Full Text Available The on-line detection of pipeline spillage is really essential for the fast oil spill response to the ecological and economical consequences. However existing on-line pipelines spillage detection systems have a sensibility of 0.2 – 1 % of pipe flow and do not detect the smaller-sized spillages.For unpeopled or sparsely populated regions an advanced technique for detection of pipeline spillages (including low-intensity ones is to monitor oil pollution (petroleum spills on the earth surface along the pipeline using, for example, an air drone.The laser remote sensing method is an effective method to detect the pipelines spillage.The paper is dedicated to development of laser fluorescence detection method of oil pollution. The remote sensing laser method to monitor oil pollution is based on the fluorescence excitation of oil in UV spectral band and on the data record of the earth surface laser-induced fluorescence radiation.For laser fluorescence method of monitoring oil pollution the paper presents a comparative analysis  of 0.266 and 0.355 µm wavelengths of the fluorescence excitation in terms of earth atmosphere propagation, eye-safety, laser characteristics, and petroleum fluorescence excitation efficiency.It is shown that in terms of eye-safety, laser characteristics, and propagation in the earth atmosphere a 0.355 µm laser wavelength of the fluorescence excitation has a sure advantage.In the context of petroleum fluorescence excitation efficiency a 0.266 µm laser wavelength of the fluorescence excitation has the advantage, but this advantage depends heavily on the petroleum base. For low-sulfur (sweet oil for instance,  it is not that big.At large, in solving the task of oil pollution detection because of the oil pipeline spillages the 0.355 µm wavelength of fluorescence excitation ought to be preferable. However, when creating a monitoring system for the pipeline with a specific petroleum base the irreversible decision depends on the fluorescence excitation efficiency at 0.266 and 0.355 µm wavelengths of fluorescence excitation for a specific petroleum base.

Environmental Management Performance Report May 2000

International Nuclear Information System (INIS)

EDER, D.M.

2000-01-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-FU) a report of the Project Hanford Management Contractors' (PHMC)' Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. This report is a monthly publication that summarizes the PHMC EM performance. In addition, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual Project (e.g., Waste Management, Spent Nuclear Fuels, etc.), in support of Section A of the report. A glossary of terms is provided at the end of this report for reference purposes. Unless otherwise noted, the Safety, Conduct of Operations, Metrics, and Cost/Schedule data contained herein is as of March 31, 2000. All other information is updated as noted

Environmental Management Performance Report May 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-05-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-FU) a report of the Project Hanford Management Contractors' (PHMC)' Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. This report is a monthly publication that summarizes the PHMC EM performance. In addition, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual Project (e.g., Waste Management, Spent Nuclear Fuels, etc.), in support of Section A of the report. A glossary of terms is provided at the end of this report for reference purposes. Unless otherwise noted, the Safety, Conduct of Operations, Metrics, and Cost/Schedule data contained herein is as of March 31, 2000. All other information is updated as noted.

TWRS FSAR integrated control decision meetings - January 22 - 31,1997

Energy Technology Data Exchange (ETDEWEB)

Saladin, V.L.

1997-03-17

U.S. Department of Energy (DOE), Richland Operations Office (RL) letter 97-MSD-163 dated January 15, 1997, directed the Project Hanford Management Contractor (Contractor), Fluor Daniel Hanford, inc., to form a joint RL-Contractor Integrated Control Decision Team (ICDT) to evaluate the Tank Waste Remediation System (TWRS) Final Safety Analysis Report (FSAR) accident scenarios that were identified to be above the risk evaluation guidelines (radiological and/or toxicological) defined by the April 8, 1996, letter from J. Kinzer, RL-TWRS (96-MSO-069) to Dr. A. L. Trego, Westinghouse Hanford Company. The ICDT evaluated six postulated accidents from the draft FSAR which had analyzed consequences above the DOE directed risk evaluation guidelines after controls were applied. The accidents were: (1) Organic Solvent Fires; (2) Organic Salt-Nitrate Fire; (3) Spray Leak; (4) Flammable Gas; (5) Steam Intrusion; and (6) Seismic Event. Five of the postulated accidents exceed radiological risk guidelines. Although the postulated steam intrusion accident does not exceed the radiological risk guidelines, it was considered in the ICDT evaluation because its calculated consequences exceed toxicological risk evaluation guidelines. Figure 1 delineates the mitigated and unmitigated risk evaluations performed for the FSAR.

SNF sludge treatment system preliminary project execution plan

International Nuclear Information System (INIS)

Flament, T.A.

1998-01-01

The Fluor Daniel Hanford, Inc. (FDH) Project Director for the Spent Nuclear Fuel (SNF) Project has requested Numatec Hanford Company (NHC) to define how Hanford would manage a new subproject to provide a process system to receive and chemically treat radioactive sludge currently stored in the 100 K Area fuel retention basins. The subproject, named the Sludge Treatment System (STS) Subproject, provides and operates facilities and equipment to chemically process K Basin sludge to meet Tank Waste Remediation System (TWRS) requirements. This document sets forth the NHC management approach for the STS Subproject and will comply with the requirements of the SNF Project Management Plan (HNF-SD-SNFPMP-011). This version of this document is intended to apply to the initial phase of the subproject and to evolve through subsequent revision to include all design, fabrication, and construction conducted on the project and the necessary management and engineering functions within the scope of the subproject. As Project Manager, NHC will perform those activities necessary to complete the STS Subproject within approved cost and schedule baselines and turn over to FDH facilities, systems, and documentation necessary for operation of the STS

Environmental Management Performance Report March 2000

International Nuclear Information System (INIS)

EDER, D.M.

2000-01-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-RL) a report of the Project Hanford Management Contractors' (PHMC) Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. This report is a monthly publication that summarizes the PHMC EM performance. In addition, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual mission area (e.g., Waste Management, Spent Nuclear fuels , etc.), in support of Section A of the report. A glossary of terms is provided at the end of this report for reference purposes. Unless otherwise noted, the Safety, Conduct of Operations, and Cost/schedule data contained herein is as of January 31, 2000. All other information is as of March 1, 2000

Actinide, lanthanide and fission product speciation and electrochemistry in high and low temperature ionic melts

Energy Technology Data Exchange (ETDEWEB)

Bhatt, Anand I.; Kinoshita, Hajime; Koster, Anne L.; May, Iain; Sharrad, Clint A.; Volkovich, Vladimir A.; Fox, O. Danny; Jones, Chris J.; Lewin, Bob G.; Charnock, John M.; Hennig, Christoph

2004-07-01

There is currently a great deal of research interest in the development of molten salt technology, both classical high temperature melts and low temperature ionic liquids, for the electrochemical separation of the actinides from spent nuclear fuel. We are interested in gaining a better understanding of actinide and key fission product speciation and electrochemical properties in a range of melts. Our studies in high temperature alkali metal melts (including LiCl and LiCl-KCl and CsCl-NaCl eutectics) have focussed on in-situ species of U, Th, Tc and Ru using X-ray absorption spectroscopy (XAS, both EXAFS and XANES) and electronic absorption spectroscopy (EAS). We report unusual actinide speciation in high temperature melts and an evaluation of the likelihood of Ru or Tc volatilization during plant operation. Our studies in lower temperature melts (ionic liquids) have focussed on salts containing tertiary alkyl group 15 cations and the bis(tri-fluor-methyl)sulfonyl)imide anion, melts which we have shown to have exceptionally wide electrochemical windows. We report Ln, Th, U and Np speciation (XAS, EAS and vibrational spectroscopy) and electrochemistry in these melts and relate the solution studies to crystallographic characterised benchmark species. (authors)

Actinide, lanthanide and fission product speciation and electrochemistry in high and low temperature ionic melts

International Nuclear Information System (INIS)

Bhatt, Anand I.; Kinoshita, Hajime; Koster, Anne L.; May, Iain; Sharrad, Clint A.; Volkovich, Vladimir A.; Fox, O. Danny; Jones, Chris J.; Lewin, Bob G.; Charnock, John M.; Hennig, Christoph

2004-01-01

There is currently a great deal of research interest in the development of molten salt technology, both classical high temperature melts and low temperature ionic liquids, for the electrochemical separation of the actinides from spent nuclear fuel. We are interested in gaining a better understanding of actinide and key fission product speciation and electrochemical properties in a range of melts. Our studies in high temperature alkali metal melts (including LiCl and LiCl-KCl and CsCl-NaCl eutectics) have focussed on in-situ species of U, Th, Tc and Ru using X-ray absorption spectroscopy (XAS, both EXAFS and XANES) and electronic absorption spectroscopy (EAS). We report unusual actinide speciation in high temperature melts and an evaluation of the likelihood of Ru or Tc volatilization during plant operation. Our studies in lower temperature melts (ionic liquids) have focussed on salts containing tertiary alkyl group 15 cations and the bis(tri-fluor-methyl)sulfonyl)imide anion, melts which we have shown to have exceptionally wide electrochemical windows. We report Ln, Th, U and Np speciation (XAS, EAS and vibrational spectroscopy) and electrochemistry in these melts and relate the solution studies to crystallographic characterised benchmark species. (authors)

Hanford K basins spent nuclear fuel project update

International Nuclear Information System (INIS)

Williams, N.H.; Hudson, F.G.

1997-07-01

Twenty one hundred metric tons of spent nuclear fuel (SNF) are currently stored in the Hanford Site K Basins near the Columbia River. The deteriorating conditions of the fuel and the basins provide engineering and management challenges to assure safe current and future storage. DE and S Hanford, Inc., part of the Fluor Daniel Hanford, Inc. lead team on the Project Hanford Management Contract, is constructing facilities and systems to move the fuel from current pool storage to a dry interim storage facility away from the Columbia River, and to treat and dispose of K Basins sludge, debris and water. The process starts in K Basins where fuel elements will be removed from existing canisters, washed, and separated from sludge and scrap fuel pieces. Fuel elements will be placed in baskets and loaded into Multi-Canister Overpacks (MCOs) and into transportation casks. The MCO and cask will be transported to the Cold Vacuum Drying Facility, where free water within the MCO will be removed under vacuum at slightly elevated temperatures. The MCOs will be sealed and transported via the transport cask to the Canister Storage Building

Devonian climate and reef evolution: Insights from oxygen isotopes in apatite

Science.gov (United States)

Joachimski, M. M.; Breisig, S.; Buggisch, W.; Talent, J. A.; Mawson, R.; Gereke, M.; Morrow, J. R.; Day, J.; Weddige, K.

2009-07-01

Conodonts, microfossils composed of carbonate-fluor apatite, are abundant in Palaeozoic-Triassic sediments and have a high potential to preserve primary oxygen isotope signals. In order to reconstruct the palaeotemperature history of the Devonian, the oxygen isotope composition of apatite phosphate was measured on 639 conodont samples from sequences in Europe, North America and Australia. The Early Devonian (Lochkovian; 416-411 Myr) was characterized by warm tropical temperatures of around 30 °C. A cooling trend started in the Pragian (410 Myr) with intermediate temperatures around 23 to 25 °C reconstructed for the Middle Devonian (397-385 Myr). During the Frasnian (383-375 Myr), temperatures increased again with temperatures to 30 °C calculated for the Frasnian-Famennian transition (375 Myr). During the Famennian (375-359 Myr), surface water temperatures slightly decreased. Reconstructed Devonian palaeotemperatures do not support earlier views suggesting the Middle Devonian was a supergreenhouse interval, an interpretation based partly on the development of extensive tropical coral-stromatoporoid communities during the Middle Devonian. Instead, the Devonian palaeotemperature record suggests that Middle Devonian coral-stromatoporoid reefs flourished during cooler time intervals whereas microbial reefs dominated during the warm to very warm Early and Late Devonian.

Three-dimensional in vitro measurements of tooth wear using fluoridated dentifrices.

Science.gov (United States)

Al-Mashhadani, A; Plygkos, I; Bozec, L; Rodriguez, J M

2016-09-01

The aim of this study was to compare differences in wear of human enamel and dentine in vitro using a 3D measurement method comparing silica versus non-silica containing fluoridated dentifrices (Colgate Total(™) [CT] or Fluor Protector Gel(™) [FPG]). Mounted native enamel (n = 36) and polished dentine (n = 36) samples were subjected to 10 wear cycles. Each cycle consisted of: (1) 1 hour remineralization in artificial saliva (AS); (2) 10 minute erosion (0.3% citric acid; pH = 2.8); (3) 2 minute toothbrush abrasion in AS (G1, control) or a slurry of 3:1 by weight of AS:dentifrice (G2 = CT; G3 = FPG) under a load of 2 N. Each group contained 12 enamel and 12 dentine samples. Paired pre- and post-wear scans made with a contacting scanner were digitally superimposed using ball bearings as datum. Mean and (SD) enamel wear was G1 = 21.9 μm (6.4); G2 = 15.2 μm (2.8); G3 = 16.9 μm (3.2). Enamel wear was not different between dentifrices (p = 0.99). Both dentifrices resulted in less enamel wear compared to the control (p Dental Association.

Fluoride varnish reduces white spot lesions during orthodontic treatment.

Science.gov (United States)

Shafi, Imran

2008-01-01

This was a randomised controlled trial (RCT) set in a community dental practice. The test varnish was a commercially available product, Fluor Protector (Ivoclar Vivadent, Schaan, Liechtenstein), containing 0.1% fluoride as difluorosilane in a polyurethane varnish base. The placebo varnish applied had an identical composition but without fluoride. The incidence and prevalence of white spot lesions (WSL) on the upper incisors, cuspids and premolars were recorded, as scored from digital photographs by two independent examiners. In the case of disagreement, cases were re-examined until a consensus was achieved. The incidence of WSL during the treatment period was 7.4% in the fluoride varnish group compared with 25.3% placebo group (P <0.001). The mean progression score was significantly lower in the fluoride varnish group than in the placebo group, (0.8 +/- 2.0 vs 2.6 +/- 2.8; P <0.001). The absolute risk reduction was 18% and the number-needed-to-treat was calculated to be 5.5 (95% confidence interval, 3.7-10.9). The results strongly suggest that regular topical fluoride varnish applications may reduce the development of WSL adjacent to the bracket base during treatment with fixed appliances.

Extension of Light-Harvesting Ability of Photosynthetic Light-Harvesting Complex 2 (LH2) through Ultrafast Energy Transfer from Covalently Attached Artificial Chromophores.

Science.gov (United States)

Yoneda, Yusuke; Noji, Tomoyasu; Katayama, Tetsuro; Mizutani, Naoto; Komori, Daisuke; Nango, Mamoru; Miyasaka, Hiroshi; Itoh, Shigeru; Nagasawa, Yutaka; Dewa, Takehisa

2015-10-14

Introducing appropriate artificial components into natural biological systems could enrich the original functionality. To expand the available wavelength range of photosynthetic bacterial light-harvesting complex 2 (LH2 from Rhodopseudomonas acidophila 10050), artificial fluorescent dye (Alexa Fluor 647: A647) was covalently attached to N- and C-terminal Lys residues in LH2 α-polypeptides with a molar ratio of A647/LH2 ≃ 9/1. Fluorescence and transient absorption spectroscopies revealed that intracomplex energy transfer from A647 to intrinsic chromophores of LH2 (B850) occurs in a multiexponential manner, with time constants varying from 440 fs to 23 ps through direct and B800-mediated indirect pathways. Kinetic analyses suggested that B800 chromophores mediate faster energy transfer, and the mechanism was interpretable in terms of Förster theory. This study demonstrates that a simple attachment of external chromophores with a flexible linkage can enhance the light harvesting activity of LH2 without affecting inherent functions of energy transfer, and can achieve energy transfer in the subpicosecond range. Addition of external chromophores, thus, represents a useful methodology for construction of advanced hybrid light-harvesting systems that afford solar energy in the broad spectrum.

Environmental Management Performance Report March 2000

Energy Technology Data Exchange (ETDEWEB)

EDER, D.M.

2000-03-16

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-RL) a report of the Project Hanford Management Contractors' (PHMC) Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. This report is a monthly publication that summarizes the PHMC EM performance. In addition, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual mission area (e.g., Waste Management, Spent Nuclear fuels , etc.), in support of Section A of the report. A glossary of terms is provided at the end of this report for reference purposes. Unless otherwise noted, the Safety, Conduct of Operations, and Cost/schedule data contained herein is as of January 31, 2000. All other information is as of March 1, 2000.

Results of HWVP transuranic process waste treatment laboratory and pilot-scale filtration tests using specially ground zeolite

International Nuclear Information System (INIS)

Eakin, D.E.

1996-03-01

Process waste streams from the Hanford Waste Vitrification Plant (HWVP) may require treatment for cesium, strontium, and transuranic (TRU) element removal in order to meet criteria for incorporation in grout. The approach planned for cesium and strontium removal is ion exchange using a zeolite exchanger followed by filtration. Filtration using a pneumatic hydropulse filter is planned to remove TRU elements which are associated with process solids and to also remove zeolite bearing the cesium and strontium. The solids removed during filtration are recycled to the melter feed system to be incorporated into the HWVP glass product. Fluor Daniel, Inc., the architect-engineering firm for HWVP, recommended a Pneumatic Hydropulse (PHP) filter manufactured by Mott Metallurgical Corporation for use in the HWVP. The primary waste streams considered for application of zeolite contact and filtration are melter off-gas condensate from the submerged bed scrubber (SBS), and equipment decontamination solutions from the Decontamination Waste Treatment Tank (DWTT). Other waste streams could be treated depending on TRU element and radionuclide content. Laboratory and pilot-scale filtration tests were conducted to provide a preliminary assessment of the adequacy of the recommended filter for application to HWVP waste treatment

Decision making in Brazil and emerging technologies: the case of 18F-FDG

International Nuclear Information System (INIS)

Sousa, Willy Hoppe de

2013-01-01

The article recalls the history of the development of Fluor FDG in Brazil. Important facts that impacted this development and how this technology evolved considering a time span of more then ten years, starting from 1996 is presented in this paper. Five decisions, taken between 2004 and 2005, were selected and analyzed from the perspective of knowledge that a key decision maker has developed around the main elements of a decision - problem, objectives, alternatives, consequences, risks approach and linked decisions. Contextual aspects that influenced these decisions, such as the evolution of the technology efficiency, installation of new equipment in hospitals and the consequences associated with these decisions, such as daily production capacity, distance service and numbers of attended clients are part of this study. In conclusion, this case shows that experienced decision makers can make quality decisions when they are equipped with the appropriate information, align the relevant decisions taken over time, know how to use the right tactics at the right time and with all participants in decision making. Experienced decision makers identify opportunities where there seems to be problems, review the current strategies and visualize new strategies, prepare themselves adequately to deal with the uncertainties. (author)

What Do Managers Really Want?

International Nuclear Information System (INIS)

Haberstok, J.K.

1997-01-01

Fluor Daniel Hanford, Inc. (FDH) is the Management Contractor for the Project Hanford Management Contract for the Department of Energy - Richland Operations Office. The Central Training Organization within FDH provides cross-cutting training services for its own personnel as well as for six subcontractors and six enterprise companies performing project work across the 560 square miles of the Hanford Site near Richland, Washington. Manager Safety Training has been presented by the Environmental, Safety and Health Training (ES and HT) team to managers, supervisors, team leads, and other personnel across the Hanford Site for over four years. The training is intended to heighten the awareness of management toward both identified (via accident/injury reports) and potential safety issues and concerns. Managers need to be aware of their responsibilities and to know where to go/who to contact (for example, company manuals or facility safety representatives) for additional information. At the conclusion of each training session, students are asked to complete a Level I evaluation (Kirkpatrick Model, see sidebar) and this feedback is reviewed by the instructors Commonly, revisions to the course content and presentation format are made solely by the ES and HT instructors and their manager each year

Hanford spent nuclear fuel project update

Energy Technology Data Exchange (ETDEWEB)

Williams, N.H.

1997-08-19

Twenty one hundred metric tons of spent nuclear fuel (SNF) are currently stored in the Hanford Site K Basins near the Columbia River. The deteriorating conditions of the fuel and the basins provide engineering and management challenges to assure safe current and future storage. DE and S Hanford, Inc., part of the Fluor Daniel Hanford, Inc. lead team on the Project Hanford Management Contract, is constructing facilities and systems to move the fuel from current pool storage to a dry interim storage facility away from the Columbia River, and to treat and dispose of K Basins sludge, debris and water. The process starts in K Basins where fuel elements will be removed from existing canisters, washed, and separated from sludge and scrap fuel pieces. Fuel elements will be placed in baskets and loaded into Multi-Canister Overpacks (MCOs) and into transportation casks. The MCO and cask will be transported to the Cold Vacuum Drying Facility, where free water within the MCO will be removed under vacuum at slightly elevated temperatures. The MCOs will be sealed and transported via the transport cask to the Canister Storage Building.

TWRS Final Safety Analysis Report (FSAR) integrated control decision team (ICDT) meetings January 22 - 31,1997

International Nuclear Information System (INIS)

Saladin, V.L.

1997-01-01

U.S. Department of Energy (DOE), Richland Operations Office (RL) letter 97-MSD-163 dated January 15, 1997, directed the Project Hanford Management Contractor (Contractor), Fluor Daniel Hanford, inc., to form a joint RL-Contractor Integrated Control Decision Team (ICDT) to evaluate the Tank Waste Remediation System (TWRS) Final Safety Analysis Report (FSAR) accident scenarios that were identified to be above the risk evaluation guidelines (radiological and/or toxicological) defined by the April 8, 1996, letter from J. Kinzer, RL-TWRS (96-MSO-069) to Dr. A. L. Trego, Westinghouse Hanford Company. The ICDT evaluated six postulated accidents from the draft FSAR which had analyzed consequences above the DOE directed risk evaluation guidelines after controls were applied. The accidents were: (1) Organic Solvent Fires; (2) Organic Salt-Nitrate Fire; (3) Spray Leak; (4) Flammable Gas; (5) Steam Intrusion; and (6) Seismic Event. Five of the postulated accidents exceed radiological risk guidelines. Although the postulated steam intrusion accident does not exceed the radiological risk guidelines, it was considered in the ICDT evaluation because its calculated consequences exceed toxicological risk evaluation guidelines. Figure 1 delineates the mitigated and unmitigated risk evaluations performed for the FSAR

The role of rare earth oxide nanoparticles in suppressing the photobleaching of fluorescent organic dyes

Science.gov (United States)

Guha, Anubhav; Basu, Anindita

2013-03-01

Organic dyes are widely used for both industrial as well as in scientific applications such as the fluorescent tagging of materials. However the process of photobleaching can rapidly degrade dye fluorescence rendering the material non-functional. Thus exploring novel methods for preventing photobleaching can have widespread benefits. In this work we show that the addition of minute quantities of rare earth (RE) oxide nanoparticles can significantly suppress the photobleaching of dyes. The fluorescence of Rhodamine and AlexaFluor dyes was measured as a function of time with and without the addition of CeO2 and La2O3 nanoparticle additives (two RE oxides that contain an oxygen vacancy based defect structure), as well as with FeO nanoparticles (which has an oxygen excess stoichiometry). We find that the rare earth oxides significantly prolonged the lifetimes of the dyes. The results allow us to develop a model based upon the presence of oxygen vacancies defects that allow the RE oxides to act as oxygen scavengers. This enables the RE oxide particles to effectively remove reactive oxygen free radicals generated in the dye solutions during the photoabsorption process. Current affiliation: Harvard University

Vadose Zone Hydrogeology Data Package for Hanford Assessments

Energy Technology Data Exchange (ETDEWEB)

Last, George V.; Freeman, Eugene J.; Cantrell, Kirk J.; Fayer, Michael J.; Gee, Glendon W.; Nichols, William E.; Bjornstad, Bruce N.; Horton, Duane G.

2006-06-01

This data package documents the technical basis for selecting physical and geochemical parameters and input values that will be used in vadose zone modeling for Hanford assessments. This work was originally conducted as part of the Characterization of Systems Task of the Groundwater Remediation Project managed by Fluor Hanford, Inc., Richland, Washington, and revised as part of the Characterization of Systems Project managed by the Pacific Northwest National Laboratory (PNNL) for the U.S. Department of Energy, Richland Operations Office (DOE-RL). This data package describes the geologic framework, the physical, hydrologic, and contaminant transport properties of the geologic materials, and deep drainage (i.e., recharge) estimates, and builds on the general framework developed for the initial assessment conducted using the System Assessment Capability (SAC) (Bryce et al. 2002). The general approach for this work was to update and provide incremental improvements over the previous SAC data package completed in 2001. As with the previous SAC data package, much of the data and interpreted information were extracted from existing documents and databases. Every attempt was made to provide traceability to the original source(s) of the data or interpretations.

Environmental Management Performance Report to DOE-RL September 2001

International Nuclear Information System (INIS)

EDER, D.M.

2001-01-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (RL) a monthly summary of the Central Plateau Contractor's Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the contractor baseline. The remaining sections provide detailed performance data relative to each individual Project (e.g., Waste Management, Spent Nuclear Fuels, etc.), in support of Section A of the report. Unless otherwise noted, the Safety, Conduct of Operations, and Cost/Schedule data contained herein is as of July 31, 2001. All other information is updated as of August 22, 2001 unless otherwise noted. ''Stoplight'' boxes are used to indicate at a glance the condition of a particular area. Green boxes denote on schedule. Yellows denote behind schedule but recoverable. Red is either missed or unrecoverable, without agreement by the regulating party

Prospective Comparison of F-18 Choline PET/CT Scan Versus Axial MRI for Detecting Bone Metastasis in Biochemically Relapsed Prostate Cancer Patients

Directory of Open Access Journals (Sweden)

Wouter Huysse

2017-10-01

Full Text Available We compared fluor-18 choline positron emission tomography/computed tomography (PET/CT and axial skeleton magnetic resonance imaging (MRI prospectively obtained for the detection of bone metastases in non-castrated patients with biochemically recurrent prostate cancer following primary treatment. PET/CT was performed 45 min post-injection of 3–4 MBq/kg F-18 methyl choline. MRI included T1- and fluid sensitive T2-weighted images of the spine and pelvis. Readers were initially blinded from other results and all scans underwent independent double reading. The best valuable comparator (BVC defined the metastatic status. On the basis of the BVC, 15 out of 64 patients presented with 24 bone metastases. On a patient level, the sensitivity and specificity of MRI and PET were not significantly different. On a lesion level, the sensitivity of MRI was significantly better compared to PET, and the specificity did not differ significantly. In conclusion, axial MRI is an interesting screening tool for the detection of bone metastases because of its low probability of false negative results. However, F-18 choline PET is a valuable addition as it can overrule false positive MRI results and detect non-axial metastases.

Specialized beam diagnostic measurements for an ADTT accelerator funnel

Energy Technology Data Exchange (ETDEWEB)

Gilpatrick, J.D.

1995-10-01

Los Alamos National Laboratory has proposed several CW-proton-beam facilities for accelerator-driven transmutation technologies (ADTT) with beam-current densities greater than 5 mA/mm{sup 2}. The primary beam-diagnostics-instrumentation requirement for these facilities is to provide sufficient beam information to understand and minimize beam-loss. To accomplish this task, the beam diagnostics instrumentation must measure beam parameters such as the projected centroids and profiles, total integrated current, and particle loss. Because of the high specific energy loss in materials at beam energies less than 20 MeV, interceptive measurements such as wire scanners or fluors cannot be used to determine beam profiles or centroids. Therefore, noninterceptive techniques must be used for on-line diagnosis of high-intensity CW beam at low energies. The beam funnel area of these proposed accelerator facilities provide a particular interesting beam measurement challenge. In this area of the accelerator, beam measurements must also sense how well the two funnel-input-beams are matched to each other in phase space. This paper will discuss some of the measurement requirements for these proposed accelerator facilities and the various noninterceptive techniques to measure dual-beam funnel operation.

Study of the molecular structure of uranium hexafluoride; Contribution a l'etude de la structure moleculaire de l'hexafluorure d'uranium

Energy Technology Data Exchange (ETDEWEB)

Bougon, R [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1967-06-01

The vibrational spectrum of uranium hexafluoride has been studied in both the gaseous and solid states. The study of gaseous UF{sub 6} confirms the regular octahedral structure of the fluorine atoms around the central U atom and makes it possible to evaluate some of the vibrational frequencies. From these, some new force constants have been determined. A tetragonal distortion is observed on solid UF{sub 6}; this distortion has only observed up till now by means of X-ray diffraction and nuclear magnetic resonance techniques. (author) [French] Le spectre de vibration de l'hexafluorure d'uranium UF{sub 6} est etudie sous les formes gazeuse et solide. L'etude de l'UF{sub 6} gaz confirme la structure d'octaedre regulier d'atomes de fluor autour de l'atome central d'uranium et apporte une precision sur certaines frequences de vibration. A partir de ces valeurs, de nouvelles determinations de constantes de force ont ete realisees. L'observation de UF{sub 6} solide confirme la deformation tetragonale de l'octaedre, deformation observee jusqu'a present par les seules methodes de resonance magnetique nucleaire et diffraction des rayons X. (auteur)

Modification of sulphide catalysts for hydro-treatment by addition of fluorine; Modification de catalyseurs sulfure pour l'hydrotraitement par ajout de fluor

Energy Technology Data Exchange (ETDEWEB)

Fischer, L.

1999-12-15

Ni, Mo and NiMo sulphide catalysts supported on alumina were modified with fluorine in the range of 0.8 to 17 weight % F and tested in ortho-xylene hydrogenation under 60 bar total pressure and in presence of 1 bar H{sub 2}S. A positive effect of fluorine on Ni and NiMo catalytic activity was found. The tested catalysts were characterised by electronic microscopy and X-ray-photoelectron spectroscopy. The observed variations in dispersion and sulfidation degree of the active phase are not important enough to explain the good catalytic activity. The catalytic test was adapted in a way that allows a determination of electronic effects on sulphide catalysts under typical hydro-treating conditions. The product distribution in cis- and trans- 1,2-dimethyl-cyclohexane was found to be sensitive to the electronic state of the catalyst's active site. This was verified by addition of electron-donating NH{sub 3} as well as other evidences. According to this test, fluorine acts as an electron-donator on Ni and NiMo catalysts' active sites. A volcano curve of catalytic activity in function of electronic density is obtained for MoS{sub 2} based catalysts (Mo and NiMo), suggesting the existence of an optimum electronic density which would be achieved by introduction of about 6 weight % F into a NiMo catalyst. Characterization by infrared spectroscopy of adsorbed CO confirms the electron-donating effect of fluorine. Fluoridation tests of bulk catalysts permitted to exclude a bonding between fluorine and the active sulphide phase in absence of a support. It is suggested that electron-donating fluoride located on the surface of alumina, the interaction with nickel being of van-der-Waals type. (author)

PENGGUNAAN SILVER DIAMINA FLUORIDA (SDF 38% SEBAGAI Arresting Caries Treatment (ACT PADA ANAK-ANAK

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Lendrawati Lendrawati

2011-09-01

Full Text Available AbstrakKaries merupakan masalah kesehatan gigi yang banyak diderita oleh anak-anak seluruh dunia terutama negara berkembang termasuk Indonesia. Kerusakan gigi pada anak-anak terjadi lebih cepat dibandingkan orang dewasa karena gigi yang baru erupsi masih dalam proses maturasi dan proses mineralisasi belum sempurna. Tubuli dentin anak anak yang masih lebar menyebabkan pembentukan jaringan sklerotik tidak sempurna dan buffer saliva masih kurang sehingga aktivitas proteolitik menjadi lebih banyak di dalam mulut. Fluor merupakan zat mineral yang digunakan sebagai bahan yang efektif mencegah terjadinya karies gigi dapat membuat lapisan email tahan terhadap kerusakan yang disebabkan pelarutan email oleh zat asam. Strategi pencegahan karies lebih efektif sejak diperkenalkannya silver diamina fluoride (SDF yang merupakan cairan tidak berwarna mengandung ion fluoride yang digunakan untuk memacu terjadinya proses remineralisasi hidoksiapatit mineral gigi. Penggunaan SDF ini merupakan metoda Arresting Caries Treatment (ACT. SDF menggabungkan efek penguatan gigi dari natrium fluoride (NaF dan efek nitrat perak.Konsentrasi efektif solusi SDF 38% (44.800 ion fluoride ppm digunakan untuk menghambat perkembangan karies pada gigi sulung anak-anak, terutama anak-anak yang sulit untuk dilakukan perawatan. SDF sederhana, mudah dalam mengaplikasikan dan biaya pemakaian lebih murah. SDF merupakan bahan yang tepat untuk digunakan untuk mengatasi masalah kesehatan gigi masyarakat terutama pada anak-anak.Kata Kunci : karies gigi, silver diamine fluoride, Arresting Caries Treatment, topikalAbstractDental caries is a health problem that affects many children all over the world, especially in developing countries, including Indonesia. Tooth decay in children occurs more rapidly than adults because the new tooth eruption is still in the process of maturation and mineralization process is not perfect. Dentin tubules of children is still wide lead sclerotic tissue formation

Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro

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Septriana Putri

2015-09-01

Full Text Available Abstrak Candida albicansb (C. albicans adalah salah satu mikroorganisme penyebab masalah kesehatan reproduksi wanita, yaitu keputihan (fluor albus. Penggunaan larutan povidone iodine dan bahan alam seperti ekstrak daun sirih menjadi pilihan masyarakat sebagai pembersih alat kewanitaan. Tujuan penelitian ini adalah untuk membandingkandaya hambat larutan antiseptik povidone iodine dan ekstrak daun sirih terhadap jamur C. albicans secara in vitro. Penelitian dilakukan terhadap lima isolat jamur C. albicans dengan larutan kontrol akuades.Perlakuan terdiri dari povidone iodine, ekstrak daun sirih dengan konsentrasi 5%, 10%, dan 20%.Hasil penelitian menunjukkan bahwa povidone iodine memiliki daya hambat terhadap C. albicans. Ekstrak daun sirih dengan konsentrasi 5% dan 10% tidak memiliki daya hambat terhadap C. albicans, namun ekstrak daun sirih konsentrasi 20% memiliki daya hambat terhadap C. albicans. Analisis statistik dengan uji ANOVA yang dilanjutkan dengan uji Post-hoc menunjukkan perbedaan bermakna antara daya hambat larutan povidone iodine dan ekstrak daun sirih 20% terhadap kontrol(p < 0.05.Larutan povidone iodine memiliki daya hambat dua kali lebih besar terhadap pertumbuhan C. albicans dibandingkan ekstrak daun sirih 20%. Dari penelitian ini dapat disimpulkan bahwa larutan povidone iodine dan ekstrak daun sirih 20% dapat menghambat pertumbuhan jamur C. albicans secara in vitro. Kata kunci: povidone iodine, ekstrak daun sirih, Candida albicansAbstract Candida albicans (C. albicans is one of the frequent causes of  reproductive health problems in women, namely vaginal discharge (fluor albus. The antiseptic solution, povidone iodine, is still an option to overcome vaginal discharge. The use of natural materials such as betel (Piper betle L. leaves extract also become a popular choice as adouche for women. The objective of this study was to compare the inhibitory activity of povidone iodine solution and betel leaf extract against the growth of

Efficacy of various topical agents to prevent enamel demineralization

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Priska Lestari Hendrawan

2011-09-01

Full Text Available Background: Enamel demineralization is a common and undesirable side effect of fixed appliance orthodontic treatment. Many sudies showed that the prevalence varied between 2–96%. There are many ways to prevent demineralization and increased remineralization such as oral hygiene instruction and by topical application such as acidulated phosphate fluor (APF casein phospo peptide-amorphous calcium phosphate (CPP-ACP, casein phospo peptide-amorphous calcium phosphate plus (CPP-ACPF. Purpose: The purpose of this in-vitro study was to evaluate the efficacy of various topical agents to prevent enamel demineralization. Methods: Fourty extracted human premolars were allocated to 1 of 4 groups: 1.23% APF gel; 10% CPP-ACP paste; 10% CPP-ACPF paste; and untreated control. All samples were subjected to pH cycling treatment for 12 days through a daily procedure of demineralization solution with pH 4 for 6 hours and remineralization solution with pH 7 for 18 hours. Microhardness testing were done before and after pH cycling and the delta hardness values were determined. Results: APF, CPP-ACP and CPP-ACPF application significantly prevent lowering of enamel microhardness value compared with untreated control group. Kruskal-Wallis, ANOVA, Mann-Whitney U, Tukey and Bonferroni Post-Hoc multiple comparison test showed significant difference between mean delta microhardness value of CPP-ACPF and CPP-ACP group with APF group, but there is no significant difference between mean delta microhardness value of CPP-ACPF and CPP-ACP group. Conclusion: APF, CPP-ACP and CPP-ACPF prevent enamel demineralization. CPP-ACP and CPP-ACPF prevent demineralization more than APF.Latar belakang: Demineralisasi email merupakan efek samping negatif yang sering dijumpai pada perawatan ortodontik cekat. Beberapa penelitian menyatakan bahwa prevalensinya bervariasi 2–96 persen. Ada beberapa cara untuk mencegah demineralisasi dan meningkatkan remineralisasi, misalnya dengan instruksi

PERBEDAAN TOPICAL FLUORIDE APPLICATION DAN FISSURE SEALANT DALAM MENCEGAH KARIES PADAGIGI MOLAR SATU

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Pawarti Pawarti

2017-07-01

Full Text Available Abstract: Topical Fluoride Application And Fissure Sealant To Prevent Dental Caries Permanent First Molars. Anatomy pits and fissures of teeth are caries initiation vulnerable areas of the permanent molars that grow at the age of 6 years where children can not perform oral hygiene. Caries prevention can be done by closing the pits and fissures or application of fluoride (topical fluoride application on the surface of the teeth. The purpose of this study was to analyze the difference fissure sealants and topical application of fluoride to prevent dental caries of first permanent molars. This study was a quasi-experimental study with time series, a sample was taken by purposive 117-second grade students of SDN District of North Pontianak. Data were analyzed using t-test. The result showed that fissure sealant more effective in caries prevention of first permanent molar than topical fluoride application where there were none caries teeth after and month fissure sealant, 25% sealant partially off and 11% fully off. Teeth that have done fluoride after 6 months of 3.9% of dental caries, the eighth month of 5.4% of dental caries. There was a significant difference between the effectiveness of fissure sealants with topical application of fluoride to prevent dental caries in first permanent molars p-value < 0.05, after 6-month p-value: 0.004 and after 8-month p-value: 0.001. Abstrak: Topical Fluoride Application Dan Fissure Sealant Untuk Mencegah Karies Pada Gigi Molar Satu Permanen. Anatomi pit dan fisura gigi merupakan daerah rentan inisiasi karies gigi molar satu permanen yang tumbuh pada usia 6 tahun anak belum bisa melakukan kebersihan mulutnya. Pencegahan karies dapat dilakukan dengan cara menutup pit dan fissure atau pengolesan fluor (topical fluoride application pada permukaan gigi. Tujuan penelitian ini untuk menganalisis perbedaan efektivitas fissure sealant dan topical fluoride application untuk mencegah karies gigi molar satu permanen anak

Study of the elimination of fluorine from drinking water using adsorbent materials; Estudio de la eliminacion de fluor del agua potable utilizando materiales adsorbentes

Energy Technology Data Exchange (ETDEWEB)

Flores de la Torre, J.A.; Badillo A, V.E.; Badillo A, V. [UAZ, 98600 Guadalupe, Zacatecas (Mexico); Lopez D, F.A. [Unidad PET/Ciclotron, Facultad de Medicina, UNAM, 04510 Mexico D.F. (Mexico)]. E-mail: ebadillo@cantera.reduaz.mx

2004-07-01

With the purpose of diminishing the levels of fluorine in the water in certain areas geographical of the country, the interaction of the fluorine is studied, with a Mexican natural clay, called kaolinite and a synthetic apatite called hydroxyapatite. Due to the discharges concentrations of this element in waters of human consumption cause fluorosis dental and osseous, it is important to propose adsorbent materials able to diminish those elevated concentrations of fluorine. In this investigation work the retention of the fluorine is studied in mineral phases using the tracer radioactive {sup 8} F. This retention is expressed in terms of the fixed percent of {sup 18} F, in a natural kaolinite in solution of NaCl 0.01 M, and in a synthetic hydroxyapatite setting in contact with a solution of NaF 0.01 M and a solution of NaH{sub 2}PO{sub 4} 0.01 M, all in function of the value of the p H of the solution. The results demonstrate that the influence of the p H is remarkable in the retention of the fluoride in both minerals, demonstrating that the hydroxyapatite (calcium phosphate) it retains in a lot of bigger proportion to the fluorine that the kaolinite (aluminosilicate), all this to values of acid p H, diminishing as the value of the p H increases. (Author)

Determinação da concentração de fluor em agua de consumo e em bebidas comercializadas no Estado de Santa Catarina

OpenAIRE

Ferreira, Rinaldo

1995-01-01

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciencias Agrarias Verificou-se, através do método eletrométrico, a concentração de flúor na água de consumo público tratada e não tratada e em bebidas comercializadas no estado de Santa Catarina. Nas 150 amostras de bebidas analisadas foi encontrada uma concentração média de 0,498 ppm de flúor com um desvio padrão de 0,583. Com relação à àgua tratada 48,71% das amostras apresentaram níveis de flúor insuficiente, 35,...

Environmental Management Performance Report September 2000

International Nuclear Information System (INIS)

EDER, D.M.

2000-01-01

The purpose of this report is to provide the Department of Energy Richland Operations Office (DOE-RL) a monthly summary of the Project Hanford Management Contractor's (PHMC) Environmental Management (EM) performance by Fluor Hanford (FH) and its subcontractors. In addition to project-specific information, it includes some PHMC-level data not detailed elsewhere in the report. Section A, Executive Summary, provides an executive level summary of the cost, schedule, and technical performance described in this report. It summarizes performance for the period covered, highlights areas worthy of management attention, and provides a forward look to some of the upcoming key performance activities as extracted from the PHMC baseline. The remaining sections provide detailed performance data relative to each individual Project (e.g., Waste Management, Spent Nuclear Fuels, etc.), in support of Section A of the report. Unless otherwise noted, the Safety, Conduct of Operations, and Cost/Schedule data contained herein is as of July 31, 2000. All other information is updated as of August 24, unless otherwise noted. ''Stoplight'' boxes are used to indicate at a glance the condition of a particular area. Green boxes denote on schedule. Yellows denote behind schedule but recoverable. Red is either missed or unrecoverable

Replication Banding Patterns in Human Chromosomes Detected Using 5-ethynyl-2'-deoxyuridine Incorporation

International Nuclear Information System (INIS)

Hoshi, Osamu; Ushiki, Tatsuo

2011-01-01

A novel technique using the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) into replicating DNA is described for the analysis of replicating banding patterns of human metaphase chromosomes. Human lymphocytes were synchronized with excess thymidine and treated with EdU during the late S phase of the cell cycle. The incorporated EdU was then detected in metaphase chromosomes using Alexa Fluor® 488 azides, through the 1,3-dipolar cycloaddition reaction of organic azides with the terminal acetylene group of EdU. Chromosomes with incorporated EdU showed a banding pattern similar to G-banding of normal human chromosomes. Imaging by atomic force microscopy (AFM) in liquid conditions showed that the structure of the chromosomes was well preserved even after EdU treatment. Comparison between fluorescence microscopy and AFM images of the same chromosome 1 indicated the presence of ridges and grooves in the chromatid arm, features that have been previously reported in relation to G-banding. These results suggest an intimate relationship between EdU-induced replication bands and G- or R-bands in human chromosomes. This technique is thus useful for analyzing the structure of chromosomes in relation to their banding patterns following DNA replication in the S phase

Targeting personalized medicine in a non-Hodgkin lymphoma patient with {sup 18F}-FDG and {sup 18F}-choline PET/CT

Energy Technology Data Exchange (ETDEWEB)

Ribeiro, Thalles H.; Filho, Raul S.; Castro, Ana Carolina G.; Paulino Junior, Eduardo; Mamede, Marcelo, E-mail: mamede.mm@gmail.com [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil)

2017-02-15

Early diagnosis and staging of non-Hodgkin lymphoma (NHL) is essential for therapeutic strategy decision. Positron emission tomography/computed tomography (PET/CT) with fluorodeoxyglucose (FDG), a glucose analogue, labeled with fluor-18 ({sup 18F}-FDG) has been used to evaluate staging, therapy response and prognosis in NHL patients. However, in some cases, {sup 18F}-FDG has shown false- -positive uptake due to inflammatory reaction after chemo and/or radiation therapy. In this case report, we present a NHL patient evaluated with {sup 18F}-FDG and {sup 18F}-choline PET/CT scan imaging pre- and post-therapy. {sup 18F}-FDG and {sup 18F}-choline PET/CT were performed for the purpose of tumor staging and have shown intense uptake in infiltrative tissue as well as in the lymph node, but with some mismatching in the tumor. Post-treatment {sup 18F}-FDG and {sup 18F}-choline PET/ CT scans revealed no signs of radiotracer uptake, suggesting complete remission of the tumor. {sup 18F}-choline may be a complimentary tool for staging and assessment of therapeutic response in non-Hodgkin lymphoma, while non-{sup 18F}-FDG tracer can be used for targeted therapy and patient management. (author)

Waste-gas damage to fruit in the vicinity of a fluorine plant

Energy Technology Data Exchange (ETDEWEB)

Daessler, H G; Grumbach, H

1967-01-01

Little attention has been paid to fluor-containing emissions and their effects on agriculture, fruit-growing, and horticulture. Effects of such emissions on species and varieties of fruits are described by the example of a fluorine plant. Acute or chronic damage was determined by meteorological and climatic conditions. Findings suggested that fruits are greatly damaged by the continued influence of fluorine-containing waste-gas, mainly in dry and hot years when the trees suffered from serious water deficit. It was found that necroses are formed under these conditions, whereas significant leaf lesions had to be ascribed to acute effects of such emissions. The amount of damage depended on the stage of vegetation and the physiological activity of of the fruit species and varieties concerned. No dependency was found to exist between the amount of fluorine in the fruits and the extent of the necroses. The causes of varying reactions in the different species and varieties have not been clarified as yet. Both the characteristic appearance and extent of damage observed in fruit necroses were found to differ by varieties. A detailed description is given on the appearance of damage. Fruit necroses resulted in considerable decline of quality level and in harvesting losses which are explained by examples.

Mineralogical and geochemical characteristics of uranium-rich fluoriteinEl-Missikat mineralized granite,Central Eastern Desert, Egypt

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Mohamed Fahmy Raslan

2009-12-01

Full Text Available A unique, highly radioactive variety of fluor it emineral has been recorded in the uranium occurrence of El-Missikat sheared granite pluton. In this occurrence, the uranium assumes different forms, including its presence as discrete, visible, secondary minerals, rare uraninite and its association with the jasperoid and silica veinlets. However,in some other parts of the sheared zone, the uranium was found to be solely incorporated with fluorite crystals,filling veinlet sand fractures with out any other manife station.This paper focuses ont her elevant mineralogical and geochemical characteristics of this unique fluorite variety.In addition to an investigation with binocular and polarizing microscopes, the separated fluorite grains were analyzed usingan environmental scanning electronmicroscope(ESEM and a field-emission scanning electron microscope.In addition to this,some fluorite crystals were subjected to electron microprobe analyses. While the fluorite accounted for as much as 20% of the sheared granite samples studied, it was found to range from 82 to 96 % in the different size fractions of the separated heavy mineral content. In some parts of the separated fluorite crystals,uranium inquantities of up to 2200 ppm was found to be heterogeneously distributed in the fluoritelattice,regardless of its coloration.

Fluorescent Labeling of Collagen Production by Cells for Noninvasive Imaging of Extracellular Matrix Deposition.

Science.gov (United States)

Bardsley, Katie; Yang, Ying; El Haj, Alicia J

2017-04-01

Extracellular matrix (ECM) is an essential component of tissues and provides both integrity and biological cues for cells. Collagen is one of the major proteins found within the ECM and therefore is an essential component of all engineered tissues. Therefore, in this article, we present a method for the online real-time monitoring of collagen deposition in three-dimensional engineered constructs. This method revolves around modification of collagen through the addition of azide-L-proline to cell culture media. The incorporation of azide-L-proline into the neocollagen produced by cells can then be detected by reaction with 10 mM of a Click-IT Alexa Fluor 488 DIBO Alkyne. The reaction was shown as being specific to the collagen as little background staining was observed in cultures, which did not contain the modified proline, and the staining was also depleted after treatment with collagenase and colocalization of collagen type I staining by immunochemistry assay. Real-time online staining of collagen deposition was observed under different culture conditions without affecting proliferation. Collagen deposition was observed to be increased under mechanical stimulation; however, the localization varied across stimulation regimes. This is a new technique for real-time monitoring of cell-produced collagen and will be a valuable addition to the tissue engineering field.

Adsorption studies in a fluorinated atmosphere; Etude de l'adsorption en atmosphere fluorante

Energy Technology Data Exchange (ETDEWEB)

Abassin, J J; Barberi, P; Guillouet, Y; Hartmanshenn, O; Lambard, J; Machefer, J; Michel, J [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1966-03-01

This CEA report deals with the adaptation of conventional or non-conventional apparatus to the measurement of the physical and chemical adsorption of corrosive fluorine-containing gases. Various techniques are reviewed, in particular: - thermogravimetry; - volumetry; - use of radio-active tracers; - calorimetry; - hertzian spectroscopy; - infrared spectroscopy. In each of these cases, problems of corrosion call for the use of special techniques which require the extensive use of pure nickel and aluminium or certain of their alloys. Diagrams of the apparatus and some examples of applications are given, together with some details of the performances obtained and of the main drawbacks. (authors) [French] Ce rapport CEA traite de l'adaptation d'appareillages classiques ou non a la mesure de l'adsorption physique et chimique de derives fluores gazeux corrosifs. Differentes techniques sont passees en revue, en particulier: - la thermogravimetrie; - la volumetrie; - l'utilisation de traceurs radioactifs; - la calorimetrie; - la spectroscopie hertzienne; - la spectroscopie infra-rouge. Dans chacun des cas, les problemes de corrosion conduisent a une technologie specialement adaptee et utilisant tres largement le nickel et l'aluminium purs ou certains de leurs alliages. Des schemas d'appareils et quelques exemples d'application sont proposes, ainsi que quelques precisions sur les performances obtenues et les principaux defauts. (auteurs)

Fast Flux Test Facility (FFTF) standby plan

Energy Technology Data Exchange (ETDEWEB)

Hulvey, R.K.

1997-03-06

The FFTF Standby Plan, Revision 0, provides changes to the major elements and project baselines to maintain the FFTF plant in a standby condition and to continue washing sodium from irradiated reactor fuel. The Plan is consistent with the Memorandum of Decision approved by the Secretary of Energy on January 17, 1997, which directed that FFTF be maintained in a standby condition to permit the Department to make a decision on whether the facility should play a future role in the Department of Energy`s dual track tritium production strategy. This decision would be made in parallel with the intended December 1998 decision on the selection of the primary, long- term source of tritium. This also allows the Department to review the economic and technical feasibility of using the FFTF to produce isotopes for the medical community. Formal direction has been received from DOE-RL and Fluor 2020 Daniel Hanford to implement the FFTF standby decision. The objective of the Plan is maintain the condition of the FFTF systems, equipment and personnel to preserve the option for plant restart within three and one-half years of a decision to restart, while continuing deactivation work which is consistent with the standby mode.

Real-time visualization of macromolecule uptake by epidermal Langerhans cells in living animals.

Science.gov (United States)

Frugé, Rachel E; Krout, Colleen; Lu, Ran; Matsushima, Hironori; Takashima, Akira

2012-03-01

As a skin-resident member of the dendritic cell family, Langerhans cells (LCs) are generally regarded to function as professional antigen-presenting cells. Here we report a simple method to visualize the endocytotic activity of LCs in living animals. BALB/c mice received subcutaneous injection of FITC-conjugated dextran (DX) probes into the ear skin and were then examined under confocal microscopy. Large numbers of FITC(+) epidermal cells became detectable 12-24 hours after injection as background fluorescence signals began to disappear. Most (>90%) of the FITC(+) epidermal cells expressed Langerin, and >95% of Langerin(+) epidermal cells exhibited significant FITC signals. To assess intracellular localization, Alexa Fluor 546-conjugated DX probes were locally injected into IAβ-enhanced green fluorescent protein (EGFP) knock-in mice and Langerin-EGFP-diphtheria toxin receptor mice--three dimensional rotation images showed close association of most of the internalized DX probes with major histocompatibility complex (MHC) class II molecules, but not with Langerin molecules. These observations support the current view that LCs constantly sample surrounding materials, including harmful and innocuous antigens, at the environmental interface. Our data also validate the potential utility of the newly developed imaging approach to monitor LC function in wild-type animals.

Glycan characterization of the NIST RM monoclonal antibody using a total analytical solution: From sample preparation to data analysis.

Science.gov (United States)

Hilliard, Mark; Alley, William R; McManus, Ciara A; Yu, Ying Qing; Hallinan, Sinead; Gebler, John; Rudd, Pauline M

Glycosylation is an important attribute of biopharmaceutical products to monitor from development through production. However, glycosylation analysis has traditionally been a time-consuming process with long sample preparation protocols and manual interpretation of the data. To address the challenges associated with glycan analysis, we developed a streamlined analytical solution that covers the entire process from sample preparation to data analysis. In this communication, we describe the complete analytical solution that begins with a simplified and fast N-linked glycan sample preparation protocol that can be completed in less than 1 hr. The sample preparation includes labelling with RapiFluor-MS tag to improve both fluorescence (FLR) and mass spectral (MS) sensitivities. Following HILIC-UPLC/FLR/MS analyses, the data are processed and a library search based on glucose units has been included to expedite the task of structural assignment. We then applied this total analytical solution to characterize the glycosylation of the NIST Reference Material mAb 8761. For this glycoprotein, we confidently identified 35 N-linked glycans and all three major classes, high mannose, complex, and hybrid, were present. The majority of the glycans were neutral and fucosylated; glycans featuring N-glycolylneuraminic acid and those with two galactoses connected via an α1,3-linkage were also identified.

[Clinical and microbiological study regarding surface antibacterial properties of bioactive dental materials].

Science.gov (United States)

Târcă, T; Bădescu, Aida; Topoliceanu, C; Lăcătuşu, St

2010-01-01

In the new era of dentistry the coronal restoration materials must possess "bio-active" features represented by fluor ions release, chemical adhesion and antibacterial agents. Our study aims to determine the surface antibacterial properties of glassionomer cements and compomers. The study group included 64 patients with high cariogenic risk with 80 teeth with acute and chronic dental caries affecting proximal and occlusal dental surfaces. The teeth with cariogenic lesions were restored with zinc-oxide-eugenol (n=20), glassionomer cement GC Fuji Triage (n=20), glassionomer cement modified with resins Fuji II LC (n=20), compomer Dyract (n=20). DENTOCULT SM test (Orion Diagnostica, Finland) was used for bacterial analyses. The samples from bacterial biofilm were collected from the restorated dental surfaces (study group) and intact enamel surfaces (control group). The recorded data were processed using non-parametrical statistical tests. The lowest mean value of bacterial indices was recorded for glassionomer cement Fuji Triage (0.4), and Fuji II LC (1.2), material with highest surface antibacterial properties. The highest value (1.5) was recorded for compomer Dyract. The Kruskal-Wallis test proves the significant statistical differences between the three bioactive materials. The materials with bioactive features have the ability to inhibate the growth of Streptococcus mutans in bacterial biofilm to the surfaces of coronal restoration.

Development of species-specific rDNA probes for Giardia by multiple fluorescent in situ hybridization combined with immunocytochemical identification of cyst wall antigens.

Science.gov (United States)

Erlandsen, Stanley L; Jarroll, Edward; Wallis, Peter; van Keulen, Harry

2005-08-01

In this study, we describe the development of fluorescent oligonucleotide probes to variable regions in the small subunit of 16S rRNA in three distinct Giardia species. Sense and antisense probes (17-22 mer) to variable regions 1, 3, and 8 were labeled with digoxygenin or selected fluorochomes (FluorX, Cy3, or Cy5). Optimal results were obtained with fluorochome-labeled oligonucleotides for detection of rRNA in Giardia cysts. Specificity of fluorescent in situ hybridization (FISH) was shown using RNase digestion and high stringency to diminish the hybridization signal, and oligonucleotide probes for rRNA in Giardia lamblia, Giardia muris, and Giardia ardeae were shown to specifically stain rRNA only within cysts or trophozoites of those species. The fluorescent oligonucleotide specific for rRNA in human isolates of Giardia was positive for ten different strains. A method for simultaneous FISH detection of cysts using fluorescent antibody (genotype marker) and two oligonucleotide probes (species marker) permitted visualization of G. lamblia and G. muris cysts in the same preparation. Testing of an environmental water sample revealed the presence of FISH-positive G. lamblia cysts with a specific rDNA probe for rRNA, while negative cysts were presumed to be of animal or bird origin.

Effects of applying anchovy (Stolephorus insularis) substrates on the microhardness of tooth enamel in Sprague-Dawley rats

Science.gov (United States)

Hendrik, Y. C.; Puspitawati, R.; Gunawan, H. A.

2017-08-01

Anchovies (Stolephorus insularis) contain high levels of fluor in the form of CaF2. The aim of this study is to analyze changes in tooth enamel microhardness after application of anchovy substrates by feeding or as a topical fluoridation material. An in vivo study of the lower left incisors of nine Sprague-Dawley rats was conducted. The sample was comprised of baseline and treatment groups, including feeding application, topical application, negative control feeding, and negative control topical groups. The treatment groups were given 5% anchovy substrates through feeding and topical applications. After treatment, tooth samples were extracted from each of the rats for examination, and statistical analyses were performed after determining hardness numbers for enamel surfaces using Vickers microhardness tester. Vickers hardness numbers (VHNs) for anchovy substrate application and consumption by feeding (440.3 ± 24.72) were higher than for the negative control (315.80 ± 17.51). VHNs for the topical application group were higher than for the negative control (347.28 ± 28.56) and for the feeding group. The use of anchovy as a fluoridation material in form of topical application is potentially an effective method for increasing the microhardness of the tooth enamel surface

Effect of radiation-induced modification in fluoroelastomer

International Nuclear Information System (INIS)

Zen, Heloisa Augusto; Lugao, Ademar Benevolo

2013-01-01

Polymers exposed to ionizing irradiation, even at low doses, often undergo structural changes accompanied by molecular crosslinking and chain scission (degradation) reactions. The general effect of the radiation on polymers is determined by the ratio of crosslinking to chain scission events. This ratio depends on parameters such as chemical structure, physical state, radicals stability and mobility, irradiation rate and irradiation atmosphere. The radiation process is a large used technique to promote modification in their structures to apply them in different areas and is well known for its merits and potential in modifying the chemical and the physical properties of polymeric materials without cause drastic changes in their inherent properties, depend on the dose irradiated. In this study was used fluoroelastomer with 70% - fluor that having excellent thermal, chemical and mechanical properties. Vulcanized and non-vulcanized samples of this material were submitted to gamma radiation under air atmosphere in order to observe the effect of atmosphere in the polymer matrix. The irradiated doses were 5, 10 and 20kGy, at room temperature. The characterization was made by scanning electron microscope (SEM), infrared spectroscopy using attenuate reflectance (ATR-IR) and X-ray diffraction. The results demonstrated which was expected, the degradation reactions were observed. (author)

Adsorption studies in a fluorinated atmosphere; Etude de l'adsorption en atmosphere fluorante

Energy Technology Data Exchange (ETDEWEB)

Abassin, J.J.; Barberi, P.; Guillouet, Y.; Hartmanshenn, O.; Lambard, J.; Machefer, J.; Michel, J. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

1966-03-01

This CEA report deals with the adaptation of conventional or non-conventional apparatus to the measurement of the physical and chemical adsorption of corrosive fluorine-containing gases. Various techniques are reviewed, in particular: - thermogravimetry; - volumetry; - use of radio-active tracers; - calorimetry; - hertzian spectroscopy; - infrared spectroscopy. In each of these cases, problems of corrosion call for the use of special techniques which require the extensive use of pure nickel and aluminium or certain of their alloys. Diagrams of the apparatus and some examples of applications are given, together with some details of the performances obtained and of the main drawbacks. (authors) [French] Ce rapport CEA traite de l'adaptation d'appareillages classiques ou non a la mesure de l'adsorption physique et chimique de derives fluores gazeux corrosifs. Differentes techniques sont passees en revue, en particulier: - la thermogravimetrie; - la volumetrie; - l'utilisation de traceurs radioactifs; - la calorimetrie; - la spectroscopie hertzienne; - la spectroscopie infra-rouge. Dans chacun des cas, les problemes de corrosion conduisent a une technologie specialement adaptee et utilisant tres largement le nickel et l'aluminium purs ou certains de leurs alliages. Des schemas d'appareils et quelques exemples d'application sont proposes, ainsi que quelques precisions sur les performances obtenues et les principaux defauts. (auteurs)

In Vivo Dual Fluorescence Imaging to Detect Joint Destruction.

Science.gov (United States)

Cho, Hongsik; Bhatti, Fazal-Ur-Rehman; Lee, Sangmin; Brand, David D; Yi, Ae-Kyung; Hasty, Karen A

2016-10-01

Diagnosis of cartilage damage in early stages of arthritis is vital to impede the progression of disease. In this regard, considerable progress has been made in near-infrared fluorescence (NIRF) optical imaging technique. Arthritis can develop due to various mechanisms but one of the main contributors is the production of matrix metalloproteinases (MMPs), enzymes that can degrade components of the extracellular matrix. Especially, MMP-1 and MMP-13 have main roles in rheumatoid arthritis and osteoarthritis because they enhance collagen degradation in the process of arthritis. We present here a novel NIRF imaging strategy that can be used to determine the activity of MMPs and cartilage damage simultaneously by detection of exposed type II collagen in cartilage tissue. In this study, retro-orbital injection of mixed fluorescent dyes, MMPSense 750 FAST (MMP750) dye and Alexa Fluor 680 conjugated monoclonal mouse antibody immune-reactive to type II collagen, was administered in the arthritic mice. Both dyes were detected with different intensity according to degree of joint destruction in the animal. Thus, our dual fluorescence imaging method can be used to detect cartilage damage as well as MMP activity simultaneously in early stage arthritis. © 2016 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.

Bacterial adherence on fluorinated carbon based coatings deposited on polyethylene surfaces

International Nuclear Information System (INIS)

Terriza, A; Del Prado, G; Perez, A Ortiz; Martinez, M J; Puertolas, J A; Manso, D Molina; Gonzalez-Elipe, A R; Yubero, F; Barrena, E Gomez; Esteban, J

2010-01-01

Development of intrinsically antibacterial surfaces is of key importance in the context of prostheses used in orthopaedic surgery. In this work we present a thorough study of several plasma based coatings that may be used with this functionality: diamond like carbon (DLC), fluorine doped DLC (F-DLC) and a high fluorine content carbon-fluor polymer (CF X ). The study correlates the surface chemistry and hydrophobicity of the coating surfaces with their antibacterial performance. The coatings were deposited by RF-plasma assisted deposition at room temperature on ultra high molecular weight polyethylene (UHMWPE) samples. Fluorine content and relative amount of C-C and C-F bond types was monitored by X-ray photoelectron spectroscopy and hydrophobicity by water contact angle measurements. Adherence of Staphylococcus aureus and Staphylococcus epidermidis to non-coated and coated UHMWPE samples was evaluated. Comparisons of the adherence performance were evaluated using a paired t test (two materials) and a Kruskall Wallis test (all the materials). S. aureus was statistically significant (p< 0.001) less adherent to DLC and F-DLC surfaces than S. epidermidis. Both bacteria showed reduction of adherence on DLC/UHMWPE. For S. aureus, reduction of bacterial adherence on F-DLC/UHMWPE was statistically significant respect to all other materials.