WorldWideScience

Sample records for fluorescent lighting retrofits

  1. Structuring lighting retrofit projects for success

    International Nuclear Information System (INIS)

    Fetters, J.L.

    1993-01-01

    Several trends are converging to create a substantial market for lighting retrofits in these uncertain economic times. Low interest rates are making investments in buildings more attractive than alternative financial investments such as CD's. With activity in the new construction market at an all time low, savvy contractors are turning to the retrofit market - restoring, renovating and upgrading existing buildings. Heightened environmental concerns are fueling lighting incentive programs from the electric utilities and the EPA. Improvements in lighting technology are arriving in the marketplace at an accelerated rate. Building occupants are demanding higher quality workplaces and managers are seeking higher levels of productivity. Replacing older lighting equipment can substantially improve lighting efficiency, decrease energy and maintenance costs and, at the same time, improve the workplace by increasing lighting quality Lighting retrofit projects have a demonstrated track record, reducing lighting costs on the order of 50%. This experience can be applied to help structure retrofit projects. But, in order to reap all the benefits available from a retrofit project, it must be structured for success. The retrofit option introduces a unique series of issues for building owners, managers and engineers. The best way to prevent these issues from undermining the success of retrofit projects is to anticipate and completely plan for them. Successful retrofit projects run smoothER and stay within budgetary constraints. Three important considerations will guide the successful project: (1) Start comprehensive planning early, (2) Investigate existing conditions, and (3) Be sensitive to owner/occupants needs keep building operational

  2. Simulating Performance Risk for Lighting Retrofit Decisions

    Directory of Open Access Journals (Sweden)

    Jia Hu

    2015-05-01

    Full Text Available In building retrofit projects, dynamic simulations are performed to simulate building performance. Uncertainty may negatively affect model calibration and predicted lighting energy savings, which increases the chance of default on performance-based contracts. Therefore, the aim of this paper is to develop a simulation-based method that can analyze lighting performance risk in lighting retrofit decisions. The model uses a surrogate model, which is constructed by adaptively selecting sample points and generating approximation surfaces with fast computing time. The surrogate model is a replacement of the computation intensive process. A statistical method is developed to generate extreme weather profile based on the 20-year historical weather data. A stochastic occupancy model was created using actual occupancy data to generate realistic occupancy patterns. Energy usage of lighting, and heating, ventilation, and air conditioning (HVAC is simulated using EnergyPlus. The method can evaluate the influence of different risk factors (e.g., variation of luminaire input wattage, varying weather conditions on lighting and HVAC energy consumption and lighting electricity demand. Probability distributions are generated to quantify the risk values. A case study was conducted to demonstrate and validate the methods. The surrogate model is a good solution for quantifying the risk factors and probability distribution of the building performance.

  3. LED Retrofit Kits, TLEDs, and Lighting Controls: An Application Guide

    Energy Technology Data Exchange (ETDEWEB)

    None

    2017-03-01

    This document provides guidance for retrofitting existing fluorescent troffers including the LED and associated control options available, the pro/cons and costs/benefits of each option, and agency specific requirements (where applicable).

  4. Analysis of energy efficient highway lighting retrofits.

    Science.gov (United States)

    2015-06-01

    Solid state lighting technology is advancing rapidly to a point where light emitting diode (LED) lighting : systems can be viable replacements for existing lighting systems using high pressure sodium (HPS). The : present report summarizes analyses co...

  5. New Carrollton Federal Building Lighting Retrofit Captures Cool Savings

    Energy Technology Data Exchange (ETDEWEB)

    None

    2017-01-01

    Case study describes how the U.S. General Services Administration cut a $291,000 annual lighting electric bill to an estimated $53,500 by upgrading their fluorescent lighting to a new LED troffer lighting and controls system in the New Carrollton Federal Building in Lanham, Maryland. The lighting project yielded an 82% reduction in energy use and earned GSA two awards for exemplary performance from the Interior Lighting Campaign in 2016.

  6. Standard Measurement & Verification Plan for Lighting Equipment Retrofit or Replacement Projects

    Energy Technology Data Exchange (ETDEWEB)

    Richman, Eric E.

    2009-11-04

    This document provides a framework for a standard Measurement and Verification (M&V) plan for lighting projects. It was developed to support cost-effective retrofits (partial and complete replacements) of lighting systems and is intended to provide a foundation for an M&V plan for a lighting retrofit utilizing a "best practice" approach, and to provide guidance to site owners, contractors, and other involved organizations on what is essential for a robust M&V plan for lighting projects. This document provides examples of appropriate elements of an M&V plan, including the calculation of expected energy savings. The standard M&V plan, as provided, also allows for consistent comparison with other similar lighting projects. Although intended for lighting retrofit applications, M&V plans developed per this framework document may also be used for other non-lighting technology retrofits and new installations.

  7. Trial Demonstration of Area Lighting Retrofit: Yuma Border Patrol, Yuma Arizona

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, Andrea M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); McCullough, Jeffrey J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-12-01

    The Yuma Sector Border Patrol Area is a high flux lighting application in a high temperature environment, presenting a formidable challenge for light-emitting diodes (LEDs). This retrofit is an Energy Savings Performance Contract ENABLE project under the U.S. Department of Energy (DOE) Federal Energy Management Program. If high flux LED technology performs well in a region with high ambient temperature and solar radiation, it can perform well in most outdoor environments. The design process for the Yuma retrofit has already provided valuable knowledge to CBP and DOE. The LED lighting system selected for the retrofit is expected to reduce energy consumption 69% compared to the incumbent quartz metal halide (QMH) lighting system. If the LED lighting system is installed, GATEWAY will continue to document and disseminate information regarding the installation and long-term performance so that others may also gain valuable knowledge from the Yuma Sector Border Patrol Area lighting retrofit.

  8. Feasibility of Retrofitting Lifts on Commuter and Light Rail Vehicles

    Science.gov (United States)

    1980-09-01

    The objectives of this study were to determine if lift retrofit applications to rail vehicles are technically feasible, and if so, the extent to which existing bus lift technology can be utilized. This report examines some of the technical issues ass...

  9. GATEWAY Demonstrations: Trial Demonstration of Area Lighting Retrofit, Yuma Border Patrol, Yuma, Arizona

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, A. M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); McCullough, J. J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2014-12-31

    Along the Yuma Sector Border Patrol Area in Yuma, Arizona, the GATEWAY program conducted a trial demonstration in which the incumbent quartz metal halide area lighting was replaced with LED at three pole locations at the Yuma Sector Border Patrol Area in Yuma, Arizona. The retrofit was documented to better understand LED technology performance in high-temperature environments.

  10. A spreadsheet-based microcomputer application for determining cost-effectiveness of commercial lighting retrofit opportunities

    International Nuclear Information System (INIS)

    Spain, T.K.

    1992-01-01

    Lighting accounts for 20-25% of electricity use in the United States. With estimates of 50-70% potential reductions being made by energy engineers, lighting is a promising area for cost-effective energy conservation projects in commercial buildings. With an extensive array of alternatives available to replace or modify existing lighting systems, simple but effective calculation tools are needed to help energy auditors evaluate lighting retrofits. This paper describes a spreadsheet-based microcomputer application for determining the cost-effectiveness of commercial lighting retrofits. Developed to support walk-through energy audits conducted by the Industrial Energy Advisory Service (IdEAS), the spreadsheet provides essential comparative data for evaluating the payback of alternatives. The impact of alternatives on environmental emissions is calculated to help communicate external costs and sell the project, if appropriate. The methodology and calculations are fully documented to allow the user to duplicate the spreadsheet and modify it as needed

  11. Shedding Some Light on Fluorescent Bulbs.

    Science.gov (United States)

    Guilbert, Nicholas R.

    1996-01-01

    Explores some of the principles behind the working of fluorescent bulbs using a specially prepared fluorescent bulb with the white inner fluorescent coating applied along only half its length. Discusses the spectrum, the bulb plasma, and light production. (JRH)

  12. Evaluation of particulate filtration efficiency of retrofit particulate filters for light duty vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Van Asch, R.; Verbeek, R.

    2009-10-15

    In the light of the currently running subsidy programme for particulate filters in the Netherlands, the Dutch ministry of spatial planning and environment (VROM) asked TNO to execute a desk study to evaluate the particulates filtration efficiency of retrofit particulate filters for light duty vehicles (passenger cars and vans). The typical retrofit particulate filters for light duty vehicles are also called 'open' or 'half-open' filters, because a part of the exhaust gas can pass through the particulate filter unfiltered. From design point they are very different from the majority of the factory installed particulate filters, which are also called wall-flow or 'closed' particulate filters. Due to these differences there is a large difference in filtration efficiency. Whereas the 'dosed' particulate filters show a filtration efficiency of larger than 90%, the filtration efficiency of 'open' particulate filters is generally lower (type approval minimum 30%), and strongly dependent on the conditions of use. The objective of the current project was to assess the average filtration efficiency of retrofit (open) particulate fillters on light duty vehicles in real world day to day driving, based on available literature data. Also, the reasons of a possible deviation with the type approval test results (minimum filtration efficiency of 30%) was investigated.

  13. Light Sheet Fluorescence Microscopy (LSFM).

    Science.gov (United States)

    Adams, Michael W; Loftus, Andrew F; Dunn, Sarah E; Joens, Matthew S; Fitzpatrick, James A J

    2015-01-05

    The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly isotropic optical sections. However, increased axial resolution, via pinhole optics, comes at the cost of both speed and excitation efficiency. Light sheet fluorescent microscopy (LSFM), a century-old idea made possible with modern developments in both excitation and detection optics, provides sub-cellular resolution and optical sectioning capabilities without compromising speed or excitation efficiency. Over the past decade, several variations of LSFM have been implemented each with its own benefits and deficiencies. Here we discuss LSFM fundamentals and outline the basic principles of several major light-sheet-based imaging modalities (SPIM, inverted SPIM, multi-view SPIM, Bessel beam SPIM, and stimulated emission depletion SPIM) while considering their biological relevance in terms of intrusiveness, temporal resolution, and sample requirements. Copyright © 2015 John Wiley & Sons, Inc.

  14. Fluorescence lifetime imaging using light emitting diodes

    International Nuclear Information System (INIS)

    Kennedy, Gordon T; Munro, Ian; Poher, Vincent; French, Paul M W; Neil, Mark A A; Elson, Daniel S; Hares, Jonathan D

    2008-01-01

    We demonstrate flexible use of low cost, high-power light emitting diodes as illumination sources for fluorescence lifetime imaging (FLIM). Both time-domain and frequency-domain techniques have been implemented at wavelengths spanning the range 450-640 nm. Additionally, we demonstrate optically sectioned fluorescence lifetime imaging by combining structured illumination with frequency-domain FLIM

  15. Demonstration Assessment of Light-Emitting Diode (LED) Retrofit Lamps at the Lobby of the Bonneville Power Administration, Portland, OR

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Naomi

    2011-07-01

    This report describes the process and results of a demonstration of solid-state lighting (SSL) technology in the lobby of the Bonneville Power Administration (BPA) headquarters building in Portland, Oregon. The project involved a simple retrofit of 32 track lights used to illuminate historical black-and-white photos and printed color posters from the 1930s and 1940s. BPA is a federal power marketing agency in the Northwestern United States, and selected this prominent location to demonstrate energy efficient light-emitting diode (LED) retrofit options that not only can reduce the electric bill for their customers but also provide attractive alternatives to conventional products, in this case accent lighting for BPA's historical artwork.

  16. Compact Fluorescent Light Bulbs (CFLs)

    Science.gov (United States)

    CFLs can help you save money, use less energy, reduce light bulb changes, and lower greenhouse gas emissions, which lead to climate change. Learn about proper cleanup, recycling and disposal, labels, mercury, and UV radiation.

  17. Sustainable LED Fluorescent Light Replacement Technology

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2011-09-30

    Ilumisys and the National Center for Manufacturing Sciences (NCMS) partnered on a three-year project awarded by the United States (U.S.) Department of Energy (DOE), to quantify the impacts of LED lamps, incandescent lamps and fluorescent benchmark lamps over a product lifecycle – i.e. to develop a sustainable design and manufacturing strategy that addresses product manufacturing, use, recycling and disposal scenarios for LED-based lighting. Based on the knowledge gained from extensive product tear-down studies of fluorescent and screw-in lighting products, lifecycle assessment tools, and accelerated lifecycle testing protocols, an interactive Sustainable LED Design Guide has been developed to aid architectural and lighting designers and engineers in making design decisions that consider three important environmental impacts (greenhouse gas emissions, energy use and mercury emission) across all phases of the life of an LED lighting product. Critical information developed for the lifecycle analysis and product feature comparisons is the useful life of the lighting product as well as its performance. The Design Guide is available at www.ncms.org, and was developed based on operational and durability testing of a variety of lighting products including power consumption, light output, and useful life of a lamp in order to allow a more realistic comparison of lamp designs. This report describes the main project tasks, results and innovative features of the lifecycle assessment (LCA)-based design tools, and the key considerations driving the sustainable design of LED lighting systems. The Design Guide incorporates the following three novel features for efficiently evaluating LED lighting features in value-chains: Bill-of-Materials (BOM) Builder – Designers may import process data for each component and supply functional data for the product, including power, consumption, lumen output and expected useful life: Environmental Impact Review – Designs are comparable

  18. Fluorescent lighting with aluminum nitride phosphors

    Science.gov (United States)

    Cherepy, Nerine J.; Payne, Stephen A.; Seeley, Zachary M.; Srivastava, Alok M.

    2016-05-10

    A fluorescent lamp includes a glass envelope; at least two electrodes connected to the glass envelope; mercury vapor and an inert gas within the glass envelope; and a phosphor within the glass envelope, wherein the phosphor blend includes aluminum nitride. The phosphor may be a wurtzite (hexagonal) crystalline structure Al.sub.(1-x)M.sub.xN phosphor, where M may be drawn from beryllium, magnesium, calcium, strontium, barium, zinc, scandium, yttrium, lanthanum, cerium, praseodymium, europium, gadolinium, terbium, ytterbium, bismuth, manganese, silicon, germanium, tin, boron, or gallium is synthesized to include dopants to control its luminescence under ultraviolet excitation. The disclosed Al.sub.(1-x)M.sub.xN:Mn phosphor provides bright orange-red emission, comparable in efficiency and spectrum to that of the standard orange-red phosphor used in fluorescent lighting, Y.sub.2O.sub.3:Eu. Furthermore, it offers excellent lumen maintenance in a fluorescent lamp, and does not utilize "critical rare earths," minimizing sensitivity to fluctuating market prices for the rare earth elements.

  19. Light-induced fluorescence for pulpal diagnosis

    Science.gov (United States)

    Ebihara, Arata; Liaw, Lih-Huei L.; Krasieva, Tatiana B.; Wilder-Smith, Petra B. B.

    2001-04-01

    A direct non-histological means of pulpal diagnosis remains elusive to clinical practice. Clinical vitality testing remains limited to electric, thermal criteria, or laser Doppler flowmetry. The goal of these investigations was to determine the feasibility of using light-induced fluorescence as a non-invasive modality for pulpal evaluation. Such a capability would, for example, permit expanded use of pulpotomy/pulpectomy techniques. Clinically healthy and diseased human extirpated pulpal tissues were used in this study. After excision, they were rapidly frozen and standard cryosections prepared. Measurement of tissue excitation/emission characteristics was performed using spectrographic analysis. A low-light level fluorescence microscopy system was then used to image autofluorescence localization and intensity at optimal excitation/detection parameters. Excitation/detection parameters used in this study included 405/605, 405/635, 405/670, 440/550, and 440/635. Autofluorescence intensities in healthy tissues were significantly stronger than those in diseased tissues at optimal parameters. It is postulated that autofluorescence characteristics are related to pathology- related structural changes in the pulp. This work provides the basis for further investigation into the relation between autofluorescence, histology and clinical symptoms.

  20. The Light - Electric Power Services experience in the lifetime extension of 15 kV shielded manoeuvre and control device sets through the development and utilization of retrofit circuit breakers; Experiencia da Light na extensao de vida util de conjuntos de manobra e controle blindados de 15 kV atraves do desenvolvimento e utilizacao de disjuntores retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Geraldo Magella Pereira da; Carneiro, Jose Manuel Alves [Light - Servicos de Eletricidade S.A., Rio de Janeiro, RJ (Brazil)

    2000-07-01

    The paper presents the Light experience in the extension of lifetime of the 15 kV shield sets by replacing the circuit breakers by a retrofit vacuum or S F6 modern technologies, with total utilization of the lockers which are in good conditions. The following topics will be approached: development of the retrofit circuit breakers, problems and respective solutions, comparative costs, circuit breaker replacing program and reduction of the failure indexes with consequent increasing of the system reliability.

  1. Turnkey Heating, Ventilating, and Air Conditioning and Lighting Retrofit Solution Combining Energy Efficiency and Demand Response Benefits

    Energy Technology Data Exchange (ETDEWEB)

    Doebber, Ian [National Renewable Energy Lab. (NREL), Golden, CO (United States); Deru, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States); Trenbath, Kim [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-04-12

    NREL worked with the Bonneville Power Administration's Technology Innovation Office to demonstrate a turnkey, retrofit technology that combines demand response (DR) and energy efficiency (EE) benefits for HVAC and lighting in retail buildings. As a secondary benefit, we also controlled various plug loads and electric hot water heaters (EHWH). The technology demonstrated was Transformative Wave's eIQ Building Management System (BMS) automatically responding to DR signals. The BMS controlled the HVAC rooftop units (RTU) using the CATALYST retrofit solution also developed by Transformative Wave. The non-HVAC loads were controlled using both hardwired and ZigBee wireless communication. The wireless controllers, manufactured by Autani, were used when the building's electrical layout was too disorganized to leverage less expensive hardwired control. The six demonstration locations are within the Seattle metro area. Based on the assets curtailed by the BMS at each location, we projected the DR resource. We were targeting a 1.7 W/ft2 shed for the summer Day-Ahead events and a 0.7 W/ft2 shed for the winter events. While summarized in Table ES-1, only one summer DR event was conducted at Casino #2.

  2. Controlled synthesis of gold nanoparticles by fluorescent light irradiation

    International Nuclear Information System (INIS)

    Kim, Jun-Hyun; Lavin, Brian W; Burnett, Roarke D; Boote, Brett W

    2011-01-01

    A novel photochemical synthesis of size-controlled gold nanoparticles was reliably accomplished via both a direct reduction and a seeded-growth method at room temperature under the irradiation of fluorescent light. These methods utilized the intensity of fluorescent light that closely resembles daily sunlight (∼100 mW cm -2 ). This effectively allowed for the formation of gold nanoparticles with tunable sizes simply by controlling the concentration of trisodium citrate and gold chloride. The broad band fluorescent light was found to be an efficient source for inducing the formation of gold nanoparticles at ambient conditions. The size distribution and absorption property of the resulting nanoparticles were thoroughly characterized by scanning/transmission electron microscopy, dynamic light scattering, UV-visible spectroscopy and powder x-ray diffraction. This photochemical synthesis demonstrates, for the first time, the reliable preparation of gold nanoparticles at room temperature upon irradiation with fluorescent light.

  3. Light emission from compound eye with conformal fluorescent coating

    Science.gov (United States)

    Martín-Palma, Raúl J.; Miller, Amy E.; Pulsifer, Drew P.; Lakhtakia, Akhlesh

    2015-03-01

    Compound eyes of insects are attractive biological systems for engineered biomimicry as artificial sources of light, given their characteristic wide angular field of view. A blowfly eye was coated with a thin conformal fluorescent film, with the aim of achieving wide field-of-view emission. Experimental results showed that the coated eye emitted visible light and that the intensity showed a weaker angular dependence than a fluorescent thin film deposited on a flat surface.

  4. Fundamentals of fluorescence microscopy exploring life with light

    CERN Document Server

    Mondal, Partha Pratim

    2014-01-01

    This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence  factors and phenomena such as, molecular cro...

  5. Deep Residential Retrofits in East Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Boudreaux, Philip R [ORNL; Hendrick, Timothy P [ORNL; Christian, Jeffrey E [ORNL; Jackson, Roderick K [ORNL

    2012-04-01

    Executive Summary Oak Ridge National Laboratory (ORNL) is furthering residential energy retrofit research in the mixed-humid climate of East Tennessee by selecting 10 homes and guiding the homeowners in the energy retrofit process. The homeowners pay for the retrofits, and ORNL advises which retrofits to complete and collects post-retrofit data. This effort is in accordance with the Department of Energy s Building America program research goal of demonstrating market-ready energy retrofit packages that reduce home energy use by 30 50%. Through this research, ORNL researchers hope to understand why homeowners decide to partake in energy retrofits, the payback of home energy retrofits, and which retrofit packages most economically reduce energy use. Homeowner interviews help the researchers understand the homeowners experience. Information gathered during the interviews will aid in extending market penetration of home energy retrofits by helping researchers and the retrofit industry understand what drives homeowners in making positive decisions regarding these retrofits. This report summarizes the selection process, the pre-retrofit condition, the recommended retrofits, the actual cost of the retrofits (when available), and an estimated energy savings of the retrofit package using EnergyGauge . Of the 10 households selected to participate in the study, only five completed the recommended retrofits, three completed at least one but no more than three of the recommended retrofits, and two households did not complete any of the recommended retrofits. In the case of the two homes that did none of the recommended work, the pre-retrofit condition of the homes and the recommended retrofits are reported. The five homes that completed the recommended retrofits are monitored for energy consumption of the whole house, appliances, space conditioning equipment, water heater, and most of the other circuits with miscellaneous electric loads (MELs) and lighting. Thermal comfort is

  6. Hybrid fluorescent layer emitting polarized light

    Directory of Open Access Journals (Sweden)

    Mohammad Mohammadimasoudi

    2017-07-01

    Full Text Available Semiconductor nanorods have anisotropic absorption and emission properties. In this work a hybrid luminescent layer is produced based on a mixture of CdSe/CdS nanorods dispersed in a liquid crystal that is aligned by an electric field and polymerized by UV illumination. The film emits light with polarization ratio 0.6 (polarization contrast 4:1. Clusters of nanorods in liquid crystal can be avoided by applying an AC electric field with sufficient amplitude. This method can be made compatible with large-scale processing on flexible transparent substrates. Thin polarized light emitters can be used in LCD backlights or solar concentrators to increase the efficiency.

  7. Retrofitting Systems

    DEFF Research Database (Denmark)

    Rose, Jørgen

    1997-01-01

    This report gives an overview of the different retrofitting possibilities that are available today. The report looks at both external and internal systems for external wall constructions, roof constructions, floor constructions and foundations. All systems are described in detail in respect to use...

  8. Phased Retrofits in Existing Homes in Florida Phase II. Shallow Plus Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Martin, E. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-02-01

    The BAPIRC team and Florida Power and Light (FPL) electric utility pursued a pilot phased energy-efficiency retrofit program in Florida by creating detailed data on the energy and economic performance of two levels of retrofit - simple and deep. For this Phased Deep Retrofit (PDR) project, a total of 56 homes spread across the utility partner's territory in east central Florida, southeast Florida, and southwest Florida were instrumented between August 2012 and January 2013, and received simple pass-through retrofit measures during the period of March 2013 - June 2013. Ten of these homes received a deeper package of retrofits during August 2013 - December 2013.

  9. Light extraction efficiency enhancement for fluorescent SiC based white light-emitting diodes

    DEFF Research Database (Denmark)

    Ou, Haiyan; Ou, Yiyu; Argyraki, Aikaterini

    Fluorescent SiC based white light-emitting diodes(LEDs) light source, as an innovative energy-efficient light source, would even have longer lifetime, better light quality and eliminated blue-tone effect, compared to the current phosphor based white LED light source. In this paper, the yellow....... At a device level, the focus is on improving the light extraction efficiency due to the rather high refractive index of SiC by nanostructuring the surface of SiC. Both periodic nanostructures made by e-beam lithography and nanosphere lithography and random nanostructures made by self-assembled Au nanosphere...... mask and a thin layer of Al film have been investigated and all of them showed much enhanced extraction efficiency. All these good results pave the way to a very promising fluorescent SiC based white LED light source...

  10. Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging.

    NARCIS (Netherlands)

    Hoebe, R.A.; van Oven, C.H.; Gadella, Th.W.J.; Dhonukshe, P.B.; van Noorden, C.J.F.; Manders, E.M.M.

    2007-01-01

    Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy

  11. Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging

    NARCIS (Netherlands)

    Hoebe, R. A.; van Oven, C. H.; Gadella, T. W. J.; Dhonukshe, P. B.; van Noorden, C. J. F.; Manders, E. M. M.

    2007-01-01

    Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (

  12. Blue-green phosphor for fluorescent lighting applications

    Science.gov (United States)

    Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

    2005-03-15

    A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

  13. Spectroscopic Analysis of Today's Compact Fluorescent Light Bulbs

    Science.gov (United States)

    Pluhar, Edward

    2012-03-01

    In today's consumer market, there are many different light bulbs that claim to produce `natural' light. In my research, I both quantitatively and qualitatively analyzed this claim. First, utilizing a spectroscope, I compared the spectra emitted by different brands and types of compact fluorescent light (CFL) bulbs to the spectra emitted by the Sun. Once the bulbs were quantitatively analyzed, I proceeded to qualitatively analyze them by exposing subjects to the different bulbs. The subjects were asked to rate the quality of color in different pictures illuminated by each type of CFL. From these tests, I was able to determine the ``best'' CFL bulbs, and conclude whether the health risks associated with CFL bulbs outweigh the cost savings, longevity of the bulbs, and/or quality of light benefits.

  14. Remediation plan for fluorescent light fixtures containing polychlorinated biphenyls (PCBs)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-04-30

    This report describes the remedial action to achieve compliance with 29 CFR 1910 Occupational Safety and Health Administration (OSHA) requirements of fluorescent light fixtures containing PCBs at K-25 site. This remedial action is called the Remediation Plan for Fluorescent Light Fixtures Containing PCBs at the K-25 Site (The Plan). The Plan specifically discusses (1) conditions of non-compliance, (2) alternative solutions, (3) recommended solution, (4) remediation plan costs, (5) corrective action, (6) disposal of PCB waste, (7) training, and (8) plan conclusions. The results from inspections by Energy Systems personnel in 2 buildings at K-25 site and statistical extension of this data to 91 selected buildings at the K-25 site indicates that there are approximately 28,000 fluorescent light fixtures containing 47,036 ballasts. Approximately 38,531 contain PCBs and 2,799 of the 38,531 ballasts are leaking PCBs. Review of reportable occurrences at K-25 for the 12 month period of September 1990 through August 1991 shows that Energy Systems personnel reported 69 ballasts leaking PCBs. Each leaking ballast is in non-compliance with 29 CFR 1910 - Table Z-1-A. The age of the K-25 facilities indicate a continued and potential increase in ballasts leaking PCBs. This report considers 4 alternative solutions for dealing with the ballasts leaking PCBs. The advantages and disadvantages of each alternative solution are discussed and ranked using cost of remediation, reduction of health risks, and compliance with OSHA as criteria.

  15. Blue fluorescent organic light emitting diodes with multilayered graphene anode

    International Nuclear Information System (INIS)

    Hwang, Joohyun; Choi, Hong Kyw; Moon, Jaehyun; Shin, Jin-Wook; Joo, Chul Woong; Han, Jun-Han; Cho, Doo-Hee; Huh, Jin Woo; Choi, Sung-Yool; Lee, Jeong-Ik; Chu, Hye Yong

    2012-01-01

    As an innovative anode for organic light emitting devices (OLEDs), we have investigated graphene films. Graphene has importance due to its huge potential in flexible OLED applications. In this work, graphene films have been catalytically grown and transferred to the glass substrate for OLED fabrications. We have successfully fabricated 2 mm × 2 mm device area blue fluorescent OLEDs with graphene anodes which showed 2.1% of external quantum efficiency at 1000 cd/m 2 . This is the highest value reported among fluorescent OLEDs using graphene anodes. Oxygen plasma treatment on graphene has been found to improve hole injections in low voltage regime, which has been interpreted as oxygen plasma induced work function modification. However, plasma treatment also increases the sheet resistance of graphene, limiting the maximum luminance. In summary, our works demonstrate the practical possibility of graphene as an anode material for OLEDs and suggest a processing route which can be applied to various graphene related devices.

  16. Green fluorescent protein is lighting up fungal biology

    Science.gov (United States)

    Lorang, J.M.; Tuori, R.P; Martinez, J.P; Sawyer, T.L.; Redman, R.S.; Rollins, J. A.; Wolpert, T.J.; Johnson, K.B.; Rodriguez, R.J.; Dickman, M. B.; Ciuffetti, L.M.

    2001-01-01

    Prasher (42) cloned a cDNA for the green fluorescent protein (GFP) gene from the jellyfishAequorea victoria in 1992. Shortly thereafter, to the amazement of many investigators, this gene or derivatives thereof were successfully expressed and conferred fluorescence to bacteria andCaenorhabditis elegans cells in culture (10,31), followed by yeast (24, 39), mammals (40), Drosophila (66),Dictyostelium(23, 30), plants (28,49), and filamentous fungi (54). The tremendous success of GFP as a reporter can be attributed to unique qualities of this 238-amino-acid, 27-kDa protein which absorbs light at maxima of 395 and 475 nm and emits light at a maximum of 508 nm. The fluorescence of GFP requires only UV or blue light and oxygen, and therefore, unlike the case with other reporters (β-glucuronidase, β-galacturonidase, chloramphenicol acetyltransferase, and firefly luciferase) that rely on cofactors or substrates for activity, in vivo observation ofgfp expression is possible with individual cells, with cell populations, or in whole organisms interacting with symbionts or environments in real time. Complications caused by destructive sampling, cell permeablization for substrates, or leakage of products do not occur. Furthermore, the GFP protein is extremely stable in vivo and has been fused to the C or N terminus of many cellular and extracellular proteins without a loss of activity, thereby permitting the tagging of proteins for gene regulation analysis, protein localization, or specific organelle labeling. The mature protein resists many proteases and is stable up to 65°C and at pH 5 to 11, in 1% sodium dodecyl sulfate or 6 M guanidinium chloride (reviewed in references 17and 67), and in tissue fixed with formaldehyde, methanol, or glutaraldehyde. However, GFP loses fluorescence in methanol-acetic acid (3:1) and can be masked by autofluorescent aldehyde groups in tissue fixed with glutaraldehyde. Fluorescence is optimal at pH 7.2 to 8.0 (67).

  17. Protective effect of light emitting diode phototherapy on fluorescent light induced retinal damage in Wistar strain albino rats.

    Science.gov (United States)

    Ahamed Basha, A; Mathangi, D C; Shyamala, R; Ramesh Rao, K

    2014-09-01

    Artificial light at night alters retinal physiology. Several studies have shown that light emitting diode phototherapy protects the retina from the damaging effects of acute light exposure. The aim of this study has been to elucidate the protective effects of 670 nm LED light on retinal damage induced by chronic fluorescent light in Wistar rats. Male Wistar albino rats were divided into four groups: group 1 were control (CL), group 2, 3 and 4 were exposed to fluorescent light (FL), LED preexposure+fluorescent light exposure (LL) and only LED light exposure (OL) respectively. All animals were maintained in their specific exposure regime for 30 days. Fluorescent light of 1800 lx was exposed between 8 pm to 8 am. Rats were exposed to therapeutic LED light of 670 nm of 9 J/cm2 at 25 mW/cm2 for 6 min duration. Histopathological changes in the retina were studied. Animals of the FL group showed a significant reduction in the outer nuclear layer thickness and cell count in addition to the total thickness of the retina. LL group which were exposed to 670 nm LED prior to exposure to fluorescent light showed a significant decrease in the degree of damage. 670 nm LED light preexposure is protective to retinal cells against fluorescent light-induced damage. Copyright © 2014 Elsevier GmbH. All rights reserved.

  18. Ultrafast fluorescence of photosynthetic crystals and light-harvesting complexes

    NARCIS (Netherlands)

    Oort, van B.F.

    2008-01-01

    This thesis focuses on the study of photosynthetic pigment protein complexes using time resolved fluorescence techniques. Fluorescence spectroscopy often requires attaching fluorescent labels to the proteins under investigation. With photosynthetic proteins this is not necessary, because these

  19. Strengthening Building Retrofit Markets

    Energy Technology Data Exchange (ETDEWEB)

    Templeton, Mary [Michigan Saves; Jackson, Robert [Michigan Energy Office

    2014-04-15

    The Business Energy Financing (BEF) program offered commercial businesses in Michigan affordable financing options and other incentives designed to support energy efficiency improvements. We worked through partnerships with Michigan utilities, lenders, building contractors, trade associations, and other community organizations to offer competitive interest rates and flexible financing terms to support energy efficiency projects that otherwise would not have happened. The BEF program targeted the retail food market, including restaurants, grocery stores, convenience stores, and wholesale food vendors, with the goal of achieving energy efficiency retrofits for 2 percent of the target market. We offered low interest rates, flexible payments, easy applications and approval processes, and access to other incentives and rebates. Through these efforts, we sought to help customers strive for energy savings retrofits that would save 20 percent or more on their energy use. This program helped Michigan businesses reduce costs by financing energy efficient lighting, heating and cooling systems, insulation, refrigeration, equipment upgrades, and more. Businesses completed the upgrades with the help of our authorized contractors, and, through our lending partners, we provided affordable financing options.

  20. Green Fluorescent Organic Light Emitting Device with High Luminance

    Directory of Open Access Journals (Sweden)

    Ning YANG

    2014-06-01

    Full Text Available In this work, we fabricated the small molecule green fluorescent bottom-emission organic light emitting device (OLED with the configuration of glass substrate/indium tin oxide (ITO/Copper Phthalocyanine (CuPc 25 nm/ N,N’-di(naphthalen-1-yl-N,N’-diphenyl-benzidine (NPB 45 nm/ tris(8-hydroxyquinoline aluminium (Alq3 60 nm/ Lithium fluoride (LiF 1 nm/Aluminum (Al 100 nm where CuPc and NPB are the hole injection layer and the hole transport layer, respectively. CuPc is introduced in this device to improve carrier injection and efficiency. The experimental results indicated that the turn-on voltage is 2.8 V with a maximum luminance of 23510 cd/m2 at 12 V. The maximum current efficiency and power efficiency are 4.8 cd/A at 100 cd/m2 and 4.2 lm/W at 3 V, respectively. The peak of electroluminance (EL spectrum locates at 530 nm which is typical emission peak of green light. In contrast, the maximum current efficiency and power efficiency of the device without CuPc are only 4.0 cd/A at 100 mA/cm2 and 4.2 lm/W at 3.6 V, respectively.

  1. Evaluation of an LED Retrofit Project at Princeton University’s Carl Icahn Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Robert G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Murphy, Arthur L. [Princeton Univ., NJ (United States); Perrin, Tess E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-11-25

    The LED lighting retrofit at the Carl Icahn Laboratory of the Lewis-Sigler Institute for Integrative Genomics was the first building-wide interior LED project at Princeton University, following the University’s experiences from several years of exterior and small-scale interior LED implementation projects. The project addressed three luminaire types – recessed 2x2 troffers, cove and other luminaires using linear T8 fluorescent lamps, and CFL downlights - which combined accounted for over 564,000 kWh of annual energy, over 90% of the lighting energy used in the facility. The Princeton Facilities Engineering staff used a thorough process of evaluating product alternatives before selecting an acceptable LED retrofit solution for each luminaire type. Overall, 815 2x2 luminaires, 550 linear fluorescent luminaires, and 240 downlights were converted to LED as part of this project. Based solely on the reductions in wattage in converting from the incumbent fluorescent lamps to LED retrofit kits, the annual energy savings from the project was over 190,000 kWh, a savings of 37%. An additional 125,000 kWh of energy savings is expected from the implementation of occupancy and task-tuning control solutions, which will bring the total savings for the project to 62%.

  2. Heat generation and light scattering of green fluorescent protein-like pigments in coral tissue

    Science.gov (United States)

    Lyndby, Niclas H.; Kühl, Michael; Wangpraseurt, Daniel

    2016-05-01

    Green fluorescent protein (GFP)-like pigments have been proposed to have beneficial effects on coral photobiology. Here, we investigated the relationships between green fluorescence, coral heating and tissue optics for the massive coral Dipsastraea sp. (previously Favia sp.). We used microsensors to measure tissue scalar irradiance and temperature along with hyperspectral imaging and combined imaging of variable chlorophyll fluorescence and green fluorescence. Green fluorescence correlated positively with coral heating and scalar irradiance enhancement at the tissue surface. Coral tissue heating saturated for maximal levels of green fluorescence. The action spectrum of coral surface heating revealed that heating was highest under red (peaking at 680 nm) irradiance. Scalar irradiance enhancement in coral tissue was highest when illuminated with blue light, but up to 62% (for the case of highest green fluorescence) of this photon enhancement was due to green fluorescence emission. We suggest that GFP-like pigments scatter the incident radiation, which enhances light absorption and heating of the coral. However, heating saturates, because intense light scattering reduces the vertical penetration depth through the tissue eventually leading to reduced light absorption at high fluorescent pigment density. We conclude that fluorescent pigments can have a central role in modulating coral light absorption and heating.

  3. Phased Retrofits in Existing Homes in Florida Phase II: Shallow Plus Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Martin, E. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-02-03

    The BAPIRC team and Florida Power and Light (FPL) electric utility pursued a pilot phased energy-efficiency retrofit program in Florida by creating detailed data on the energy and economic performance of two levels of retrofit - simple and deep. For this Phased Deep Retrofit (PDR) project, a total of 56 homes spread across the utility partner's territory in east central Florida, southeast Florida, and southwest Florida were instrumented between August 2012 and January 2013, and received simple pass-through retrofit measures during the period of March 2013 - June 2013. Ten of these homes received a deeper package of retrofits during August 2013 - December 2013. A full account of Phase I of this project, including detailed home details and characterization, is found in Parker et al, 2015 (currently in draft). Phase II of this project, which is the focus of this report, applied the following additional retrofit measures to select homes that received a shallow retrofit in Phase I: a) Supplemental mini-split heat pump (MSHP) (6 homes); b) Ducted and space coupled Heat Pump Water Heater (8 homes); c) Exterior insulation finish system (EIFS) (1 homes); d) Window retrofit (3 homes); e) Smart thermostat (21 homes: 19 NESTs; 2 Lyrics); f) Heat pump clothes dryer (8 homes); g) Variable speed pool pump (5 homes).

  4. Fluorescent light induces neurodegeneration in the rodent nigrostriatal system but near infrared LED light does not.

    Science.gov (United States)

    Romeo, Stefania; Vitale, Flora; Viaggi, Cristina; di Marco, Stefano; Aloisi, Gabriella; Fasciani, Irene; Pardini, Carla; Pietrantoni, Ilaria; Di Paolo, Mattia; Riccitelli, Serena; Maccarone, Rita; Mattei, Claudia; Capannolo, Marta; Rossi, Mario; Capozzo, Annamaria; Corsini, Giovanni U; Scarnati, Eugenio; Lozzi, Luca; Vaglini, Francesca; Maggio, Roberto

    2017-05-01

    We investigated the effects of continuous artificial light exposure on the mouse substantia nigra (SN). A three month exposure of C57Bl/6J mice to white fluorescent light induced a 30% reduction in dopamine (DA) neurons in SN compared to controls, accompanied by a decrease of DA and its metabolites in the striatum. After six months of exposure, neurodegeneration progressed slightly, but the level of DA returned to the basal level, while the metabolites increased with respect to the control. Three month exposure to near infrared LED light (∼710nm) did not alter DA neurons in SN, nor did it decrease DA and its metabolites in the striatum. Furthermore mesencephalic cell viability, as tested by [ 3 H]DA uptake, did not change. Finally, we observed that 710nm LED light, locally conveyed in the rat SN, could modulate the firing activity of extracellular-recorded DA neurons. These data suggest that light can be detrimental or beneficial to DA neurons in SN, depending on the source and wavelength. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Eye Disease Resulting From Increased Use of Fluorescent Lighting as a Climate Change Mitigation Strategy

    Science.gov (United States)

    Walls, Kelvin L.; Benke, Geza

    2011-01-01

    Increased use of fluorescent lighting as a climate change mitigation strategy may increase eye disease. The safe range of light to avoid exposing the eye to potentially damaging ultraviolet (UV) radiation is 2000 to 3500K and greater than 500 nanometers. Some fluorescent lights fall outside this safe range. Fluorescent lighting may increase UV-related eye diseases by up to 12% and, according to our calculations, may cause an additional 3000 cases of cataracts and 7500 cases of pterygia annually in Australia. Greater control of UV exposure from fluorescent lights is required. This may be of particular concern for aging populations in developed countries and countries in northern latitudes where there is a greater dependence on artificial lighting. PMID:22021286

  6. The evaluation of a 4000-home geothermal heat pump retrofit at Fort Polk, Louisiana: Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Hughes, P.J.; Shonder, J.A.

    1998-03-01

    This report documents an independent evaluation of an energy retrofit of 4,003 family housing units at Fort Polk, Louisiana, under an energy savings performance contract (ESPC). Replacement of the heating, cooling, and water heating systems in these housing units with geothermal heat pumps (GHPs) anchored the retrofit; low-flow shower heads and compact fluorescent lighting were also installed, as well as attic insulation where needed. Statistically valid findings indicate that the project will save 25.8 million kWh, or 32.5% of the pre-retrofit whole-community electrical consumption, and 100% of the whole-community natural gas previously used for space conditioning and water heating (260,000 therms) in a typical meteorological year. At the end-use level, the GHPs were found to save about 42% of the pre-retrofit electrical consumption for heating, cooling, and water heating in housing units that were all-electric in the pre-retrofit period. This report also demonstrates an improved method of predicting energy savings. Using an engineering model calibrated to pre-retrofit energy use data collected in the field, the method predicted actual energy savings on one of the electric feeders at Fort Polk with a very high degree of accuracy. The accuracy of this model was in turn dependent on data-calibrated models of the geothermal heat pump and ground heat exchanger that are described in this report. In addition this report documents the status of vertical borehole ground heat exchanger (BHEx) design methods at the time this project was designed, and demonstrates methods of using data collected from operating GHP systems to benchmark BHEx design methods against a detailed engineering model calibrated to date. The authors also discuss the ESPC`s structure and implementation and how the experience gained here can contribute to the success of future ESPCs.

  7. Light Quality Affects Chloroplast Electron Transport Rates Estimated from Chl Fluorescence Measurements.

    Science.gov (United States)

    Evans, John R; Morgan, Patrick B; von Caemmerer, Susanne

    2017-10-01

    Chl fluorescence has been used widely to calculate photosynthetic electron transport rates. Portable photosynthesis instruments allow for combined measurements of gas exchange and Chl fluorescence. We analyzed the influence of spectral quality of actinic light on Chl fluorescence and the calculated electron transport rate, and compared this with photosynthetic rates measured by gas exchange in the absence of photorespiration. In blue actinic light, the electron transport rate calculated from Chl fluorescence overestimated the true rate by nearly a factor of two, whereas there was closer agreement under red light. This was consistent with the prediction made with a multilayer leaf model using profiles of light absorption and photosynthetic capacity. Caution is needed when interpreting combined measurements of Chl fluorescence and gas exchange, such as the calculation of CO2 partial pressure in leaf chloroplasts. © Crown copyright 2017.

  8. Temperature and light dependent modifications of chlorophyll fluorescence kinetics in spruce needles during winter.

    Science.gov (United States)

    Bolhàr-Nordenkampf, H R; Lechner, E G

    1988-11-01

    Prompt chlorophyll a fluorescence kinetics at room temperature were measured from intact spruce needles. The fluorescence signal was recorded after varying light pretreatments. During the winter, induction curves showed characteristic changes in both the initial peak of fluorescence FV/FP (FP-FO/FP) and the steady state level Fdr (FP-FT/FP). Winter stress induced decreases in both values which showed close correlation to the light and temperature pre-history of the plants. In February changes in fluorescence induction indicative of a restoration of photosynthesis were detected and these corresponded to a rise of temperature above zero in combination with low light levels. In March increasing light intensity combined with chilling temperatures induced again decreases of both values of chlorophyll fluorescence induction suggesting the occurrence of photoinhibition.

  9. Demonstration Assessment of Light-Emitting Diode (LED) Retrofit Lamps at Intercontinental Hotel in San Francisco, CA

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Naomi J.; Curry, Ku' Uipo J.

    2010-11-01

    This document is a report of observations and results obtained from a lighting demonstration project conducted under the U.S. Department of Energy (DOE) GATEWAY Demonstration Program. The program supports demonstrations of high-performance solid-state lighting (SSL) products in order to develop empirical data and experience with in-the-field applications of this advanced lighting technology. The DOE GATEWAY Demonstration Program focuses on providing a source of independent, third-party data for use in decision-making by lighting users and professionals; this data should be considered in combination with other information relevant to the particular site and application under examination. Each GATEWAY Demonstration compares SSL products against the incumbent technologies used in that location. Depending on available information and circumstances, the SSL product may also be compared to alternate lighting technologies. Though products demonstrated in the GATEWAY program have been prescreened and tested to verify their actual performance, DOE does not endorse any commercial product or in any way guarantee that users will achieve the same results through use of these products.

  10. Yuma Border Patrol Area Lighting Retrofit LED System Performance in a Trial Installation – Two Years Later

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, Andrea M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Sullivan, Gregory P. [Efficiency Solutions, Inc, Richland, WA (United States); Davis, Robert G. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-05-21

    Documentation of the Yuma Sector Border Patrol Area lighting LED trial demonstration continues to provide a better understanding of LED technology performance in a high ambient temperature and high solar radiation environment. Measured data at the project site showed illuminances changing more rapidly than anticipated. As previously predicted, the causes for these observed changes are mostly if not completely explained by dirt accumulation. The laboratory measurements showed not only the effect of dirt on lumen output, but also on the distribution of light exiting the luminaire.

  11. A new type of white light-emitting diode light source basing on fluorescent SiC

    DEFF Research Database (Denmark)

    Ou, Haiyan; Ou, Yiyu; Lu, Weifang

    Most of the commercial white light-emitting diode (LED) light sources are made from phosphor coated blue-emitting gallium nitride (GaN) chips. This type white LED light source always has tradeoff between luminous efficacy and color rendering index (CRI). Furthermore, yellow-emitting phosphor decays...... much faster than the semiconductor chip, so the white color will turn into bluish over the time. This paper will propose a new type white LED light source: using fluorescent silicon carbide (SiC) to take the place of phosphor. This new type LED has the following advantages: a) SiC is a wide bandgap...... semiconductor material , so it is stable; b) Fluorescent SiC has very wide emission spectrum, and it could generate white light with very high CRI; c) It is a better substrate than sapphire for the GaN growth in terms of lattice match and thermal conductivity. This paper will cover: the growth of fluorescent Si...

  12. White light-informed optical properties improve ultrasound-guided fluorescence tomography of photoactive protoporphyrin IX

    Science.gov (United States)

    Flynn, Brendan P.; DSouza, Alisha V.; Kanick, Stephen C.; Davis, Scott C.; Pogue, Brian W.

    2013-04-01

    Subsurface fluorescence imaging is desirable for medical applications, including protoporphyrin-IX (PpIX)-based skin tumor diagnosis, surgical guidance, and dosimetry in photodynamic therapy. While tissue optical properties and heterogeneities make true subsurface fluorescence mapping an ill-posed problem, ultrasound-guided fluorescence-tomography (USFT) provides regional fluorescence mapping. Here USFT is implemented with spectroscopic decoupling of fluorescence signals (auto-fluorescence, PpIX, photoproducts), and white light spectroscopy-determined bulk optical properties. Segmented US images provide a priori spatial information for fluorescence reconstruction using region-based, diffuse FT. The method was tested in simulations, tissue homogeneous and inclusion phantoms, and an injected-inclusion animal model. Reconstructed fluorescence yield was linear with PpIX concentration, including the lowest concentration used, 0.025 μg/ml. White light spectroscopy informed optical properties, which improved fluorescence reconstruction accuracy compared to the use of fixed, literature-based optical properties, reduced reconstruction error and reconstructed fluorescence standard deviation by factors of 8.9 and 2.0, respectively. Recovered contrast-to-background error was 25% and 74% for inclusion phantoms without and with a 2-mm skin-like layer, respectively. Preliminary mouse-model imaging demonstrated system feasibility for subsurface fluorescence measurement in vivo. These data suggest that this implementation of USFT is capable of regional PpIX mapping in human skin tumors during photodynamic therapy, to be used in dosimetric evaluations.

  13. Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains

    OpenAIRE

    Buckley, Anthony M.; Jukes, Caitlin; Candlish, Denise; Irvine, June J.; Spencer, Janice; Fagan, Robert P.; Roe, Andrew J.; Christie, John M.; Fairweather, Neil F.; Douce, Gillian R.

    2016-01-01

    The uses of fluorescent reporters derived from green fluorescent protein have proved invaluable for the visualisation of biological processes in bacteria grown under aerobic conditions. However, their requirement for oxygen has limited their application in obligate anaerobes such as Clostridium difficile. Fluorescent proteins derived from Light, Oxygen or Voltage sensing (LOV) domains have been shown to bridge this limitation, but their utility as translational fusions to monitor protein expr...

  14. Toward quantitative fluorescence by using ultrasound modulation of light

    NARCIS (Netherlands)

    Daoudi, Khalid; Steenbergen, Wiendelt

    2012-01-01

    we propose a method to quantify fluorophore absorption coefficient by combining acousto-optics and fluorescence. Using Monte Carlo simulations we explain a methodology and we show a possibility to estimate absorption coefficient within 10% accuracy

  15. A method for the rapid generation of nonsequential light-response curves of chlorophyll fluorescence.

    Science.gov (United States)

    Serôdio, João; Ezequiel, João; Frommlet, Jörg; Laviale, Martin; Lavaud, Johann

    2013-11-01

    Light-response curves (LCs) of chlorophyll fluorescence are widely used in plant physiology. Most commonly, LCs are generated sequentially, exposing the same sample to a sequence of distinct actinic light intensities. These measurements are not independent, as the response to each new light level is affected by the light exposure history experienced during previous steps of the LC, an issue particularly relevant in the case of the popular rapid light curves. In this work, we demonstrate the proof of concept of a new method for the rapid generation of LCs from nonsequential, temporally independent fluorescence measurements. The method is based on the combined use of sample illumination with digitally controlled, spatially separated beams of actinic light and a fluorescence imaging system. It allows the generation of a whole LC, including a large number of actinic light steps and adequate replication, within the time required for a single measurement (and therefore named "single-pulse light curve"). This method is illustrated for the generation of LCs of photosystem II quantum yield, relative electron transport rate, and nonphotochemical quenching on intact plant leaves exhibiting distinct light responses. This approach makes it also possible to easily characterize the integrated dynamic light response of a sample by combining the measurement of LCs (actinic light intensity is varied while measuring time is fixed) with induction/relaxation kinetics (actinic light intensity is fixed and the response is followed over time), describing both how the response to light varies with time and how the response kinetics varies with light intensity.

  16. Energy Conservation Using Scotopically Enhanced Fluorescent Lighting In An Office Environment

    Energy Technology Data Exchange (ETDEWEB)

    None

    2004-03-01

    This study was conducted in a recently built and occupied office building to determine whether the energy savings benefits of scotopically enhanced fluorescent lighting can be achieved while maintaining user acceptability.

  17. Cancer detection using NIR elastic light scattering and tissue fluorescence imaging

    Energy Technology Data Exchange (ETDEWEB)

    Demos, S G; Staggs, M; Radousky, H B; Gandour-Edwards, R; deVere White, R

    2000-12-04

    Near infrared imaging using elastic light scattering and tissue fluorescence under long-wavelength laser excitation are explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation.

  18. Building America Case Study: Pilot Demonstration of Phased Energy Efficiency Retrofits: Deep Retrofits, Central and South Florida

    Energy Technology Data Exchange (ETDEWEB)

    2017-02-22

    The Florida Solar Energy Center (FSEC), in collaboration with Florida Power & Light (FPL), is pursuing a phased residential energy-efficiency retrofit program in Florida. Researchers are looking to establish the impacts of technologies of two retrofit packages -- shallow and deep -- on annual energy and peak energy reductions.

  19. Building America Case Study: Pilot Demonstration of Phased Energy Efficiency Retrofits: Deep Retrofits, Central and South Florida

    Energy Technology Data Exchange (ETDEWEB)

    D. Parker, K. Sutherland, D. Chasar, J. Montemurno, B. Amos, J. Kono

    2017-02-01

    The Florida Solar Energy Center (FSEC), in collaboration with Florida Power & Light (FPL), is pursuing a phased residential energy-efficiency retrofit program in Florida. Researchers are looking to establish the impacts of technologies of two retrofit packages -- shallow and deep -- on annual energy and peak energy reductions.

  20. Comparison of milk oxidation by exposure to LED and fluorescent light.

    Science.gov (United States)

    Brothersen, C; McMahon, D J; Legako, J; Martini, S

    2016-04-01

    Light-induced oxidation of milk has been well studied. Exposure of milk to UV light facilitates the oxidation of fats to aldehydes, and the degradation of sulfur-containing amino acids, both of which contribute to off-flavors. In addition, vitamin A and riboflavin are easily degraded by UV light. These reactions occur rapidly and are exacerbated by bright fluorescent lights in retail dairy cases. The invention of white light-emitting diodes (LED) may provide a solution to this oxidation problem. In this study, fresh milk containing 1% fat and fortified with vitamin A and riboflavin was exposed to LED at 4,000 lx, or fluorescent light at 2,200 lx for 24 h. Milk samples exposed to LED or fluorescent light, as well as milk protected from light, were analyzed by a consumer acceptance panel, and a trained flavor panel. In addition, vitamin A, riboflavin, and the production of volatile compounds were quantified. Exposure to light resulted in a reduction of cooked/sweet, milkfat, and sweet flavors and increased the intensity of butterscotch, cardboard, and astringency. In general, exposure to fluorescent light resulted in greater changes in the milk than exposure to LED even though the LED was at higher intensity. Consumers were able detect off-flavors in milk exposed to fluorescent light after 12 h and LED after 24 h of exposure. The riboflavin and vitamin A content was reduced by exposure to fluorescent light, whereas there was no significant reduction caused by LED compared with the non-light-exposed control. Production of hexanal, heptanal, 2-heptanal, octanal, 2-octanal nonanal, dimethyl sulfide, and caproic acid vinyl ester from the light-induced degradation of fats was significantly higher with fluorescent than LED. Production of these compounds was significantly higher with both light treatments than in the control milk. This study indicates that LED is less destructive to milk than fluorescent light. Copyright © 2016 American Dairy Science Association. Published

  1. CALiPER Exploratory Study. Recessed Troffer Lighting

    Energy Technology Data Exchange (ETDEWEB)

    Miller, N. J. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Royer, M. P. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Poplawski, M. E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2013-03-01

    This CALiPER study examines the problems and benefits likely to be encountered with LED products intended to replace linear fluorescent lamps. LED dedicated troffers, replacement tubes, and non-tube retrofit kits were evaluated against fluorescent benchmark troffers in a simulated office space for photometric distribution, uniformity of light on the task surface, suitability of light output, flicker, dimming performance, color quality, power quality, safety and certification issues, ease of installation, energy efficiency, and life-cycle cost.

  2. Phased Retrofits in Existing Homes in Florida Phase I: Shallow and Deep Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Montemurno, J. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Kono, J. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-02-01

    The U.S. Department of Energy (DOE) Building America program, in collaboration with Florida Power and Light (FPL), conducted a phased residential energy-efficiency retrofit program. This research sought to establish impacts on annual energy and peak energy reductions from the technologies applied at two levels of retrofit - shallow and deep, with savings levels approaching the Building America program goals of reducing whole-house energy use by 40%. Under the Phased Deep Retrofit (PDR) project, we have installed phased, energy-efficiency retrofits in a sample of 56 existing, all-electric homes. End-use savings and economic evaluation results from the phased measure packages and single measures are summarized in this report.

  3. Phased Retrofits in Existing Homes In Florida Phase I: Shallow and Deep Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Montemurno, J. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Kono, J. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-02-04

    The U.S. Department of Energy (DOE) Building America program, in collaboration with Florida Power and Light (FPL), conducted a phased residential energy-efficiency retrofit program. This research sought to establish impacts on annual energy and peak energy reductions from the technologies applied at two levels of retrofit - shallow and deep, with savings levels approaching the Building America program goals of reducing whole-house energy use by 40%. Under the Phased Deep Retrofit (PDR) project, we have installed phased, energy-efficiency retrofits in a sample of 56 existing, all-electric homes. End-use savings and economic evaluation results from the phased measure packages and single measures are summarized in this report. Project results will be of interest to utility program designers, weatherization evaluators, and the housing remodel industry. Shallow retrofits were conducted in all homes from March to June 2013. The measures for this phase were chosen based on ease of installation, targeting lighting (CFLs and LED lamps), domestic hot water (wraps and showerheads), refrigeration (cleaning of coils), pool pump (reduction of operating hours), and the home entertainment center (smart plugs). Deep retrofits were conducted on a subset of ten PDR homes from May 2013 through March 2014. Measures included new air source heat pumps, duct repair, ceiling insulation, heat pump water heaters, variable speed pool pumps and learning thermostats. Major appliances such as refrigerators and dishwashers were replaced where they were old and inefficient.

  4. Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate.

    Science.gov (United States)

    Akimoto, Takuo; Yasuda, Mitsuru

    2010-01-01

    Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al(2)O(3) was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al(2)O(3) had two peaks and one valley in the range from 0 to 300 nm of Al(2)O(3) thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al(2)O(3) thickness as well, and the maximum reflection was observed near the Al(2)O(3) thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the lambda/4 derived by a simple interference theory.

  5. Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light.

    Science.gov (United States)

    Seiler, Franka; Soll, Jürgen; Bölter, Bettina

    2017-06-13

    Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid "ageing". This results in non-desired variations of growth conditions and lowers the comparability of data acquired over extended time periods. In this study, we investigated the growth behaviour of Arabidopsis Col0 under different light conditions, applying fluorescent compared to LED lamps, and we conducted physiological as well as gene expression analyses. By changing the spectral composition and/or light intensity of LEDs we can clearly influence the growth behaviour of Arabidopsis and thereby study phenotypic attributes under very specific light conditions that are stable and reproducible, which is not necessarily given for fluorescent lamps. By using LED lights, we can also roughly mimic the sun light emission spectrum, enabling us to study plant growth in a more natural-like light set-up. We observed distinct growth behaviour under the different light regimes which was reflected by physiological properties of the plants. In conclusion, LEDs provide variable emission spectra for studying plant growth under defined, stable light conditions.

  6. Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light

    Directory of Open Access Journals (Sweden)

    Franka Seiler

    2017-06-01

    Full Text Available Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid “ageing”. This results in non-desired variations of growth conditions and lowers the comparability of data acquired over extended time periods. In this study, we investigated the growth behaviour of Arabidopsis Col0 under different light conditions, applying fluorescent compared to LED lamps, and we conducted physiological as well as gene expression analyses. By changing the spectral composition and/or light intensity of LEDs we can clearly influence the growth behaviour of Arabidopsis and thereby study phenotypic attributes under very specific light conditions that are stable and reproducible, which is not necessarily given for fluorescent lamps. By using LED lights, we can also roughly mimic the sun light emission spectrum, enabling us to study plant growth in a more natural-like light set-up. We observed distinct growth behaviour under the different light regimes which was reflected by physiological properties of the plants. In conclusion, LEDs provide variable emission spectra for studying plant growth under defined, stable light conditions.

  7. Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope.

    Science.gov (United States)

    Wilding, Dean; Pozzi, Paolo; Soloviev, Oleg; Vdovin, Gleb; Verhaegen, Michel

    2016-10-31

    A methodology for the adaptive control and correction of phase aberrations in the illumination arm of a light-sheet fluorescence microscope has been developed. The method uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the sample. Using this signal, the aberrations in the illumination arm are subsequently corrected with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in significant improvement in the axial resolution, has been demonstrated by imaging Tg(fli:GFP) zebrafish embryos.

  8. Go with the glow: fluorescent proteins to light transgenic organisms.

    Science.gov (United States)

    Stewart, C Neal

    2006-04-01

    Once a biological novelty known for their role in bioluminescence, fluorescent proteins (FPs) from marine invertebrates have revolutionized the life sciences. Organisms from all kingdoms have been transformed with the Aequorea victoria green fluorescent protein (GFP), and biotechnology has been advanced by the use of FPs. This article reviews the current uses of FPs in whole transgenic organisms and genomics and looks beyond GFP to the complete color palette and spectral properties afforded by FPs from other marine organisms. Coupled with electronic devices for visualizing and quantifying FPs, recently cloned FP genes might be useful for the ecological monitoring of transgenic organisms in the environment. Therefore, this review also addresses the in vivo labeling of organisms with an emphasis on plants.

  9. Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light

    OpenAIRE

    Seiler, Franka; Soll, J?rgen; B?lter, Bettina

    2017-01-01

    Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid “ageing”. This results in non-desired variations of growth conditions and lowers the comparability of data acquired ove...

  10. Studies on influence of light on fluorescence of Tris-(8-hydroxyquinoline)aluminum thin films

    Science.gov (United States)

    Thangaraju, K.; Amaladass, P.; Bharathi, K. Shanmuga; Mohanakrishnan, A. K.; Narayanan, V.; Kumar, J.

    2009-03-01

    Tris-(8-hydroxyquinoline)aluminum (Alq 3) thin films, the most widely used electron transport/emissive material in the organic electroluminescent (EL) devices, have been deposited on glass substrates by thermal evaporation process. Alq 3 thin films were exposed to light for various time periods under normal ambient. The fluorescence of as-prepared and light exposed Alq 3 thin films and formation of luminescent quencher have been studied using fluorescence, Mass, MALDI-ToF-MS, 1H & 13C NMR, and FT-IR spectroscopy. It is observed that among the three 8-hydroxyquinoline (HQ) units in Alq 3 molecule, one HQ unit is affected during the light exposure in the normal ambient. It is found that the affected resultant Alq 3 molecule containing the carbonyl group acts as fluorescent quencher and the energy of excitons in the Alq 3 molecule in the light exposed Alq 3 thin films can be non-radiatively transferred to the neighboring fluorescent quencher, quenching the fluorescence of light exposed Alq 3 thin films in the normal ambient.

  11. Manipulation of cellular light from green fluorescent protein by a femtosecond laser

    Science.gov (United States)

    He, Hao; Li, Shiyang; Wang, Shaoyang; Hu, Minglie; Cao, Youjia; Wang, Chingyue

    2012-10-01

    Green fluorescent protein (GFP) is one of the most widely studied and exploited proteins in biochemistry and cell biology. It emits fluorescence following optical excitation, which is usually provided by a laser. Here, we report that fluorescence from enhanced GFP can be `turned off' by exposing cells to laser light. A short flash of femtosecond laser light is shown to deplete calcium in the endoplasmic reticulum of cells. Calcium-release-activated calcium channels are then activated by stromal interaction molecule 1 (STIM1). The rise in intracellular Ca2+ depolarizes mitochondria and increases the leakage of reactive oxygen species, which then permanently bleach the GFP. This controllable optical scheme for reactive oxygen species generation can also be used to modulate the photoconversion of GFP fluorescence from green to red emission and provide a mechanism for influencing cellular molecular dynamics.

  12. Fluorescent Brighteners as Visible LED-Light Sensitive Photoinitiators for Free Radical Photopolymerizations.

    Science.gov (United States)

    Zuo, Xiaoling; Morlet-Savary, Fabrice; Graff, Bernadette; Blanchard, Nicolas; Goddard, Jean-Philippe; Lalevée, Jacques

    2016-05-01

    The photochemical and electrochemical investigations of commercially available, safe, and cheap fluorescent brighteners, namely, triazinylstilbene (commercial name: fluorescent brightener 28) and 2,5-bis(5-tert-butyl-benzoxazol-2-yl)thiophene, as well as their original use as photoinitiators of polymerization upon light emitting diode (LED) irradiation are reported. Remarkably, their excellent near-UV-visible absorption properties combined with outstanding fluorescent properties allow them to act as high-performance photoinitiators when used in combination with diaryliodonium salt. These two-component photoinitiating systems can be employed for free radical polymerizations of acrylate. In addition, this brightener-initiated photopolymerization is able to overcome oxygen inhibition even upon irradiation with low LED light intensity. The underlying photochemical mechanisms are investigated by electron-spin resonance-spin trapping, fluorescence, cyclic voltammetry, and steady-state photolysis techniques. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Recent progress in the use of fluorescent and phosphorescent organic compounds for organic light-emitting diode lighting

    Science.gov (United States)

    Jeong, Hyocheol; Shin, Hwangyu; Lee, Jaehyun; Kim, Beomjin; Park, Young-Il; Yook, Kyoung Soo; An, Byeong-Kwan; Park, Jongwook

    2015-01-01

    Organic light-emitting diodes (OLEDs) have attracted considerable attention in both academic and industrial circles. Certain properties of OLEDs make them especially attractive in the lighting market, including area emission characteristics not found in other existing light sources, environmentally friendly efficient use of energy, large area, ultra-light weight, and ultra-thin shape. Fluorescent and phosphorescent materials that are being applied to white OLEDs have been categorized, and the chemical structures and device performances of the important blue, orange, and red light-emitting materials have been summarized. Such a systematic classification and understanding of the materials that have already been reported can aid the development and study of new light-emitting materials through quantitative and qualitative approaches.

  14. Combining electrophoresis with detection under ultraviolet light and multiple ultrafiltration for isolation of humic fluorescence fractions.

    Science.gov (United States)

    Trubetskaya, Olga E; Shaloiko, Lubov A; Demin, Dmitrii V; Marchenkov, Victor V; Proskuryakov, Ivan I; Coelho, Christian; Trubetskoj, Oleg A

    2011-04-01

    Polyacrylamide gel electrophoresis of chernozem soil humic acids (HAs) followed by observation under UV (312 nm) excitation light reveals new low molecular weight (MW) fluorescent fractions. Ultrafiltration of HAs sample in 7 M urea on a membrane of low nominal MW retention (NMWR, 5 kDa) was repetitively used for separation of fluorescent and non-fluorescent species. Thirty ultrafiltrates and the final retentate R were obtained. Fluorescence maxima of separate ultrafiltrates were different and non-monotonously changed in the range of 475-505 nm. Fluorescence maxima of less than 490 nm were detected only in the four first utrafiltrates. For further physical-chemical analyses all utrafiltrates were combined into a fraction called UFchernozem soil HAs complex. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. A compact multi-channel fluorescence sensor with ambient light suppression

    Science.gov (United States)

    Egly, Dominik; Geörg, Daniel; Rädle, Matthias; Beuermann, Thomas

    2012-03-01

    A multi-channel fluorescence sensor has been developed for process monitoring and fluorescence diagnostics. It comprises a fiber-optic set-up with an immersion probe and an intensity-modulated high power ultraviolet light-emitting diode as a light source for fluorescence excitation. By applying an electronic lock-in procedure, fluorescence signals are selectively detectable at ambient light levels of 1000 000 times higher intensity. The sensor was designed to be compact, low cost and easily adaptable to a wide field of application. The set-up was used to simultaneously monitor three important metabolic fluorophores: NAD(P)H, flavins and porphyrins during the cultivation of a baker's yeast. Moreover, the accumulation and degradation kinetics of protoporphyrin IX induced by 5-aminolevulinic acid on the skin could be recorded by the sensor. The detection limit for protoporphyrin IX was determined to be 4 × 10-11 mol L-1. The linear signal amplification of the sensor and time courses of fluorescence signals monitored during yeast fermentations were validated using a commercial CCD spectrometer. The robust and flexible set-up of the fiber-optic measurement system promises easy implementation of this non-invasive analytical tool to fluorescence monitoring and diagnostics in R&D and production.

  16. A visible-light-excited fluorescence method for imaging protein crystals without added dyes

    Science.gov (United States)

    Lukk, Tiit; Gillilan, Richard E.; Szebenyi, Doletha M. E.; Zipfel, Warren R.

    2016-01-01

    Fluorescence microscopy methods have seen an increase in popularity in recent years for detecting protein crystals in screening trays. The fluorescence-based crystal detection methods have thus far relied on intrinsic UV-inducible tryptophan fluorescence, nonlinear optics or fluorescence in the visible light range dependent on crystals soaked with fluorescent dyes. In this paper data are presented on a novel visible-light-inducible autofluorescence arising from protein crystals as a result of general stabilization of conjugated double-bond systems and increased charge delocalization due to crystal packing. The visible-light-inducible autofluorescence serves as a complementary method to bright-field microscopy in beamline applications where accurate crystal centering about the rotation axis is essential. Owing to temperature-dependent chromophore stabilization, protein crystals exhibit tenfold higher fluorescence intensity at cryogenic temperatures, making the method ideal for experiments where crystals are cooled to 100 K with a cryostream. In addition to the non-damaging excitation wavelength and low laser power required for imaging, the method can also serve a useful role for differentiating protein crystals from salt crystals in screening trays. PMID:26937240

  17. Single-molecule fluorescence microscopy review: shedding new light on old problems.

    Science.gov (United States)

    Shashkova, Sviatlana; Leake, Mark C

    2017-08-31

    Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse technique offering exceptional contrast in conjunction with high specificity of labelling with relatively minimal perturbation to biological samples compared with many competing biophysical techniques. Improvements in detector and dye technologies coupled to advances in image analysis methods have fuelled recent development towards single-molecule fluorescence microscopy, which can utilize light microscopy tools to enable the faithful detection and analysis of single fluorescent molecules used as reporter tags in biological samples. For example, the discovery of GFP, initiating the so-called 'green revolution', has pushed experimental tools in the biosciences to a completely new level of functional imaging of living samples, culminating in single fluorescent protein molecule detection. Today, fluorescence microscopy is an indispensable tool in single-molecule investigations, providing a high signal-to-noise ratio for visualization while still retaining the key features in the physiological context of native biological systems. In this review, we discuss some of the recent discoveries in the life sciences which have been enabled using single-molecule fluorescence microscopy, paying particular attention to the so-called 'super-resolution' fluorescence microscopy techniques in live cells, which are at the cutting-edge of these methods. In particular, how these tools can reveal new insights into long-standing puzzles in biology: old problems, which have been impossible to tackle using other more traditional tools until the emergence of new single-molecule fluorescence microscopy techniques. © 2017 The Author(s).

  18. Fluorescence digital photography of acne using a light-emitting diode illuminator.

    Science.gov (United States)

    Ahn, Hyo Hyun; Kim, Soo Nam; Kye, Young Chul

    2006-11-01

    The fluorescence findings of several dermatological diseases, such as erythrasma, tinea versicolor, and acne are helpful for diagnosis and follow-up. However, many experience difficulty taking photographic images of fluorescence. The aim of this study was to develop a 405 nm light-emitting diode (LED) system for fluorescence digital photography of acne and to determine whether such a diode can be used to evaluate acne. Eight healthy acne patients were compared with controls by fluorescence digital photography using a digital camera equipped with a 405 nm LED illuminator. Digital photographs were taken by two different ways of exposure, i.e. appropriate exposure level and longer exposure. One side of the nose, cheek, and glabella was compared. The numbers and extents of fluorescence dots were counted and measured. As normal controls, seven individuals with apparent oiliness and no acne were enrolled. Red fluorescent facial dots were observed and photographed digitally using the 405 nm LED illuminator. These were more numerous and extensive on the glabella and cheeks of acne patients. Fluorescence digital photography of acne was successfully performed using a 405 nm LED illuminator. This illuminator could be used for acne evaluations.

  19. Detection of proximal caries using quantitative light-induced fluorescence-digital and laser fluorescence: a comparative study.

    Science.gov (United States)

    Yoon, Hyung-In; Yoo, Min-Jeong; Park, Eun-Jin

    2017-12-01

    The purpose of this study was to evaluate the in vitro validity of quantitative light-induced fluorescence-digital (QLF-D) and laser fluorescence (DIAGNOdent) for assessing proximal caries in extracted premolars, using digital radiography as reference method. A total of 102 extracted premolars with similar lengths and shapes were used. A single operator conducted all the examinations using three different detection methods (bitewing radiography, QLF-D, and DIAGNOdent). The bitewing x-ray scale, QLF-D fluorescence loss (ΔF), and DIAGNOdent peak readings were compared and statistically analyzed. Each method showed an excellent reliability. The correlation coefficient between bitewing radiography and QLF-D, DIAGNOdent were -0.644 and 0.448, respectively, while the value between QLF-D and DIAGNOdent was -0.382. The kappa statistics for bitewing radiography and QLF-D had a higher diagnosis consensus than those for bitewing radiography and DIAGNOdent. The QLF-D was moderately to highly accurate (AUC = 0.753 - 0.908), while DIAGNOdent was moderately to less accurate (AUC = 0.622 - 0.784). All detection methods showed statistically significant correlation and high correlation between the bitewing radiography and QLF-D. QLF-D was found to be a valid and reliable alternative diagnostic method to digital bitewing radiography for in vitro detection of proximal caries.

  20. Optimum Structure Adjustment for Flexible Fluorescent and Phosphorescent Organic Light Emitting Diodes

    OpenAIRE

    Juang, Fuh-Shyang; Tsai, Yu-Sheng; Wang, Shun-Hsi; Su, Shin-Yuan; Chen, Shin-Liang; Chen, Shen-Yaur

    2010-01-01

    This research successfully improved the luminance efficiency and lifetime of flexible fluorescent and phosphorescent organic light emitting diodes by optimizing organic layer thicknesses or inserting a spin-coated buffer layer. From the results, the best phosphorescent device structure (ITO/ NPB (50nm)/ Ir(ppy)3:CBP (40nm)/ TPBi (10nm)/ Alq3 (50nm)/ LiF

  1. Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron microscopy

    NARCIS (Netherlands)

    Faas, F.G.A.; Bárcena, M.A.; Agronskaia, A.V.; Gerritsen, H.C.; Moscicka, K.B.; Diebolder, C.A.; Driel, L.F.; Limpens, R.W.A.L.; Bos, E.; Ravelli, R.B.G.; Koning, R.I.; Koster, A.J.

    2013-01-01

    Correlative light and electron microscopy is an increasingly popular technique to study complex biological systems at various levels of resolution. Fluorescence microscopy can be employed to scan large areas to localize regions of interest which are then analyzed by electron microscopy to obtain

  2. Evaluation of dental enamel caries assessment using Quantitative Light Induced Fluorescence and Optical Coherence Tomography.

    Science.gov (United States)

    Maia, Ana Marly Araújo; de Freitas, Anderson Zanardi; de L Campello, Sergio; Gomes, Anderson Stevens Leônidas; Karlsson, Lena

    2016-06-01

    An in vitro study of morphological alterations between sound dental structure and artificially induced white spot lesions in human teeth, was performed through the loss of fluorescence by Quantitative Light-Induced Fluorescence (QLF) and the alterations of the light attenuation coefficient by Optical Coherence Tomography (OCT). To analyze the OCT images using a commercially available system, a special algorithm was applied, whereas the QLF images were analyzed using the software available in the commercial system employed. When analyzing the sound region against white spot lesions region by QLF, a reduction in the fluorescence intensity was observed, whilst an increase of light attenuation by the OCT system occurred. Comparison of the percentage of alteration between optical properties of sound and artificial enamel caries regions showed that OCT processed images through the attenuation of light enhanced the tooth optical alterations more than fluorescence detected by QLF System. QLF versus OCT imaging of enamel caries: a photonics assessment. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. The TUS space fluorescence detector for study of UHECR and other phenomena of variable fluorescence light in the atmosphere

    Science.gov (United States)

    Abrashkin, V.; Alexandrov, V.; Arakcheev, Y.; Bitkin, E.; Cordero, A.; Eremin, S.; Finger, M.; Garipov, G.; Grebenyuk, V.; Kalmykov, N.; Khrenov, B.; Koval, V.; Martinez, O.; Matyushkin, A.; Moreno, E.; Naumov, D.; Olshevsky, A.; Panasyuk, M.; Park, I.; Robledo, C.; Rubinstein, I.; Sharakin, S.; Silaev, A.; Tkatchev, L.; Tulupov, V.; Tyukaev, R.; Sabirov, B.; Salazar, H.; Saprykin, O.; Syromyatnikov, V.; Urmantsev, F.; Villasenor, L.; Yashin, I.; Zaikin, N.; Zepeda, A.

    The Tracking Ultraviolet Set Up (TUS) instrument has been designed to observe from space the fluorescence light in the atmosphere when Extensive Air Shower (EAS) or other phenomena such as meteors or dust grains traverse it. The TUS design concepts will allow us to construct the next generation of fluorescence detectors with increasing light collection power and higher resolution. The KLYPVE instrument with collection power 5 times larger of the TUS will be the next space detector. Light collection is obtained with the help of segmented “low frequency Fresnel type” mirrors. Photo receiver retina in the focal consists of modules of PM tubes. For stable performance in conditions of variable light noise and variable temperature the tube type with a multi-alcali cathode was chosen. Voltage supplies for PMT in one module were designed for keeping the performance of photo receiver retina uniform when the tube gain change. From every tube the signal amplitude is recorded in time bins of 400 ns. The digital data are kept and analyzed in the module FPGA connected to the central FPGA controlling all data. The RAM memory is large, capable to record events with different duration of the light signal (up to several seconds). The preliminary event data are analyzed in the triggering system of the central FPGA. The trigger criteria have several options for events of different origin (different pixel signal duration). The trigger integration time is controlled from the space mission center. The performances of the detector were simulated and zenith angle dependent trigger efficiencies were calculated. The TUS detector will be efficient in recording “horizontal” EAS (zenith angles more than 60°), developed to their maximum above the cloud cover. The EAS Cherenkov light, back scattered from the cloud cover, will be recorded and will improve data on the EAS direction and position of maximum. For better accuracy in physical parameters of the events and for the experimental

  4. Inherent visible light signature of an intense underwater ultraviolet light source due to combined Raman and fluorescence effects

    Science.gov (United States)

    Mazel, Charles H.; Kalata-Olson, Jody; Pham, Chuong N.

    2000-07-01

    We investigated the utility of a portable, intense source of ultraviolet light for diver use in support of Very Shallow Water operations. The working hypothesis was that the light would be of use to divers at short-to-medium ranges (up to several meters) while remaining invisible to surface observers due to the incoherent insensitivity of the human eye to ultraviolet light. The light source contained an arc discharge lamp rich in short wavelengths and was fitted with a filter that transmitted only the near ultraviolet portion of the spectrum. In-water tests were made in darkness using Navy divers both in a natural coastal environment and in a test tank. It was found that the light was of limited utility to the divers. In addition, the light was not covert because of a bluish-white glow associated with the ultraviolet beam. Subsequent measurements demonstrated that the visible glow was produced by a combination of fluorescence of dissolved organic matter in the water and Raman scatter from the water itself. The relative importance of the two factors varied with water type. These two effects that transform light from the invisible to the visible impose inherent limitations on the use of ultraviolet light for covert operations.

  5. Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations

    DEFF Research Database (Denmark)

    Xie, Haiyan; Liu, Haichun; Svenmarker, Pontus

    2011-01-01

    in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law...

  6. Multifamily Ventilation Retrofit Strategies

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, K.; Lstiburek, J.; Bergey, D.

    2012-12-01

    In multifamily buildings, central ventilation systems often have poor performance, overventilating some portions of the building (causing excess energy use), while simultaneously underventilating other portions (causing diminished indoor air quality). BSC and Innova Services Corporation performed a series of field tests at a mid-rise test building undergoing a major energy audit and retrofit, which included ventilation system upgrades.

  7. A novel quantitative light-induced fluorescence device for monitoring molar-incisor hypomineralization.

    Science.gov (United States)

    Durmus, B; Durhan, A; Gökkaya, B; Kıtıki, B; Yanıkoğlu, F; Kargül, B

    2017-01-01

    The FluoreCam system is based on an innovative approach to the quantification of enamel health termed fluorescence enamel imaging (FEI). Enamel is both highly mineralized and semi-translucent. Because of its mineral composition, enamel will fluoresce when exposed to certain light wavelengths. The semi-translucent nature of enamel results in different enamel densities emitting different levels of fluorescence. As a result, with FEI technology, one can measure the density of tooth enamel by measuring its fluorescence when subjected to specific light wavelengths. To determine the ability of visual examination and the instrumental procedures of the FluoreCam to monitor molar-incisor hypomineralization (MIH) lesions. This study involved children with MIH at the Department of Paediatric Dentistry, Marmara University. In total, 11 patients with MIH were diagnosed on a visual MIH scale and evaluated with the FluoreCam. The equipment, data processing, and interaction between the equipment and operator were evaluated. Fluorescent images recorded with the custom software, the clinical view, and digital numeric values were evaluated to assess the potential for use of the device in clinical practice. These preliminary data from an ongoing clinical study suggest that measurements with the FluoreCam are useful in monitoring MIH. This technique also provides visual and quantitative feedback to patients.

  8. Acclimatization of symbiotic corals to mesophotic light environments through wavelength transformation by fluorescent protein pigments.

    Science.gov (United States)

    Smith, Edward G; D'Angelo, Cecilia; Sharon, Yoni; Tchernov, Dan; Wiedenmann, Joerg

    2017-07-12

    The depth distribution of reef-building corals exposes their photosynthetic symbionts of the genus Symbiodinium to extreme gradients in the intensity and spectral quality of the ambient light environment. Characterizing the mechanisms used by the coral holobiont to respond to the low intensity and reduced spectral composition of the light environment in deeper reefs (greater than 20 m) is fundamental to our understanding of the functioning and structure of reefs across depth gradients. Here, we demonstrate that host pigments, specifically photoconvertible red fluorescent proteins (pcRFPs), can promote coral adaptation/acclimatization to deeper-water light environments by transforming the prevalent blue light into orange-red light, which can penetrate deeper within zooxanthellae-containing tissues; this facilitates a more homogeneous distribution of photons across symbiont communities. The ecological importance of pcRFPs in deeper reefs is supported by the increasing proportion of red fluorescent corals with depth (measured down to 45 m) and increased survival of colour morphs with strong expression of pcRFPs in long-term light manipulation experiments. In addition to screening by host pigments from high light intensities in shallow water, the spectral transformation observed in deeper-water corals highlights the importance of GFP-like protein expression as an ecological mechanism to support the functioning of the coral- Symbiodinium association across steep environmental gradients. © 2017 The Authors.

  9. Nanoscale Confinement and Fluorescence Effects of Bacterial Light Harvesting Complex LH2 in Mesoporous Silicas

    DEFF Research Database (Denmark)

    Ikemoto, Hideki; Tubasum, Sumera; Pullerits, Tonu

    2013-01-01

    Many key chemical and biochemical reactions, particularly in living cells, take place in confined space at the mesoscopic scale. Towards understanding of physicochemical nature of biomacromolecules confined in nanoscale space, in this work we have elucidated fluorescent effects of a light...... harvesting complex LH2 in nanoscale chemical environments. Mesoporous silicas (SBA-15 family) with different shapes and pore sizes were synthesized and used to create nanoscale biomimetic environments for molecular confinement of LH2. A combination of UV-vis absorption, wide-field fluorescence microscopy...

  10. Broadband and omnidirectional light harvesting enhancement of fluorescent SiC

    DEFF Research Database (Denmark)

    Ou, Yiyu; Jokubavicius, Valdas; Hens, Philip

    2012-01-01

    In the present work, antireflective sub-wavelength structures have been fabricated on fluorescent 6H-SiC to enhance the white light extraction efficiency by using the reactive-ion etching method. Broadband and omnidirectional antireflection characteristics show that 6H-SiC with antireflective sub......-wavelength structures suppress the average surface reflection significantly from 20.5% to 1.01% over a wide spectral range of 390-784 nm. The luminescence intensity of the fluorescent 6H-SiC could be enhanced in the whole emission angle range. It maintains an enhancement larger than 91% up to the incident angle of 70...

  11. Comparison of Light Emitting Diodes (LED) and Fluorescent Light on Suppression of Pineal Melatonin in the Rat

    Science.gov (United States)

    Winget, Charles M.; Heeke, D. S.; Holley, D. C.; Mele, G.; Brainard, G. C.; Hanifin, J. P.; Rollag, M. D.; Savage, Paul D. (Technical Monitor)

    1997-01-01

    To validate a novel LED array for use in animal habitat lighting by comparing its effectiveness to cool-white fluorescent (CWF) lighting in suppressing pineal gland melatonin. Male Sprague-Dawley rats, 175-200 g, were maintained under control conditions for 2 weeks (food and water ad lib, 12L: 12D CWF, 18 uW/square cm). Dark adapted animals (animals before lights on) were exposed to 5 min of LED or CWF light of similar spectral power distribution. Two groups of rats (LED vs. CWF) were compared at 5 light intensities (100, 40, 1, 1.0, and 0. 1 lux). A control group was placed into the exposure apparatus but not exposed to light. After exposure, pineal glands were rapidly removed and assayed for melatonin by RIA. Results. The dark-exposed control groups matched with the 5 intensity groups (100, 40, 10, 1.0, and 0.1 lux) showed mean + SEM pineal melatonin values of 1167 +/- 136, 1569 +/- 126, 353 +/- 34, 650 +/- 124, and 464 +/- 85, pg/ml respectively. The corresponding CWF exposure data were 393 1 41, 365 +34, 257 +/- 13, 218 +/- 42, and 239 +/- 71 pg/ml, respectively. Corresponding LED exposure data were 439 +/- 25, 462 +/- 50, 231 +/- 6, 164 +/- 12, and 158 +/- 12 pg/ml, respectively. Rats exposed to both experimental light conditions at all illuminances studied showed significant melatonin suppression (p less than 0.01, ANOVA). In no case was the melatonin suppression induced by LED illuminance significantly different from the melatonin suppression elicited by the same intensity of CWF light. The results show that a novel LED light source can suppress pineal melatonin equal to that of a conventional CWF light source.

  12. Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces.

    Science.gov (United States)

    Koning, Roman I; Celler, Katherine; Willemse, Joost; Bos, Erik; van Wezel, Gilles P; Koster, Abraham J

    2014-01-01

    Light microscopy and electron microscopy are complementary techniques that in a correlative approach enable identification and targeting of fluorescently labeled structures in situ for three-dimensional imaging at nanometer resolution. Correlative imaging allows electron microscopic images to be positioned in a broader temporal and spatial context. We employed cryo-correlative light and electron microscopy (cryo-CLEM), combining cryo-fluorescence light microscopy and cryo-electron tomography, on vitrified Streptomyces bacteria to study cell division. Streptomycetes are mycelial bacteria that grow as long hyphae and reproduce via sporulation. On solid media, Streptomyces subsequently form distinct aerial mycelia where cell division leads to the formation of unigenomic spores which separate and disperse to form new colonies. In liquid media, only vegetative hyphae are present divided by noncell separating crosswalls. Their multicellular life style makes them exciting model systems for the study of bacterial development and cell division. Complex intracellular structures have been visualized with transmission electron microscopy. Here, we describe the methods for cryo-CLEM that we applied for studying Streptomyces. These methods include cell growth, fluorescent labeling, cryo-fixation by vitrification, cryo-light microscopy using a Linkam cryo-stage, image overlay and relocation, cryo-electron tomography using a Titan Krios, and tomographic reconstruction. Additionally, methods for segmentation, volume rendering, and visualization of the correlative data are described. © 2014 Elsevier Inc. All rights reserved.

  13. High-Efficiency Retrofit Lessons for Retail from a SuperTarget: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Langner, R.; Deru, M.; Hirsch, A.; Williams, S.

    2013-02-01

    The National Renewable Energy Laboratory partnered with Target under the Commercial Building Program to design and implement a retrofit of a SuperTarget in Thornton, CO. The result was a retrofit design that predicted 37% energy savings over ASHRAE Standard 90.1-2004, and 29% compared to existing (pre-retrofit) store consumption. The largest savings came from energy efficient lighting, energy efficient cooling systems, improved refrigeration, and better control of plug loads.

  14. Quantified light-induced fluorescence, review of a diagnostic tool in prevention of oral disease

    Science.gov (United States)

    de Josselin de Jong, Elbert; Higham, Susan M.; Smith, Philip W.; van Daelen, Catherina J.; van der Veen, Monique H.

    2009-05-01

    Diagnostic methods for the use in preventive dentistry are being developed continuously. Few of these find their way into general practice. Although the general trend in medicine is to focus on disease prevention and early diagnostics, in dentistry this is still not the case. Nevertheless, in dental research some of these methods seem to be promising for near future use by the general dental professional. In this paper an overview is given of a method called quantitative light-induced fluorescence or (QLF) in which visible and harmless light excites the teeth in the patient's mouth to produce fluorescent images, which can be stored on disk and computer analyzed. White spots (early dental caries) are detected and quantified as well as bacterial metabolites on and in the teeth. An overview of research to validate the technique and modeling to further the understanding of the technique by Monte Carlo simulation is given and it is shown that the fluorescence phenomena can be described by the simulation model in a qualitative way. A model describing the visibility of red fluorescence from within the dental tissue is added, as this was still lacking in current literature. An overview is given of the clinical images made with the system and of the extensive research which has been done. The QLF™ technology has been shown to be of importance when used in clinical trials with respect to the testing of toothpastes and preventive treatments. It is expected that the QLF™ technology will soon find its way into the general dental practice.

  15. Intracellular fluorescent light-up bioprobes with different morphology for image-guided photothermal cancer therapy.

    Science.gov (United States)

    Li, Bangbang; Zhang, Peng; Du, Jianwei; Zhao, Xiao; Wang, Youxiang

    2017-06-01

    Multifunctional nanoprobe was drawing increased attention in tumor diagnosis and therapy. The simple and effective establishment of the theranostic nanoplatforms was still under urgent need. Meanwhile, the targeting ability and morphology of nanoprobe were essential for the effective endocytosis, which could further affect the diagnosis. In this work, two morphologies of nanoprobes were fabricated using gold nanorods (AuNRs) and gold nanospheres (AuNSs). Thiolated-hyaluronic acid labeled with nile blue (HS-HA-NB), a near-infrared (NIR) fluorescence dye, was coated on the surface of the gold nanoparticles to form stable nanoprobes (AuNR@HS-HA-NB, AuNS@HS-HA-NB). The fluorescence of NB molecules quenched outside cells due to the fluorescence resonance energy transfer (FRET), and recovered after the HA degradation inside the cells. HA also could enhance cellular uptake in CD44 receptor highly expressed human breast carcinoma cells (MCF-7). In this way, bioprobes realized the MCF-7 cell images through intracellular fluorescent light-up. Comparing with the sphere bioprobe, the rod-shaped bioprobe dramatically promoted endocytosis to achieve a better diagnosis effect in a short time. After NIR light irradiation, severe MCF-7 apoptosis was observed with AuNR@HS-HA-NB existed. Our studies suggested that the AuNR@HS-HA-NB nanoparticles were the excellent candidates of versatile bioprobes to realize rapid, precise image and photothermal therapy to MCF-7 cells. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

    Science.gov (United States)

    Ding, Yichen; Lee, Juhyun; Ma, Jianguo; Sung, Kevin; Yokota, Tomohiro; Singh, Neha; Dooraghi, Mojdeh; Abiri, Parinaz; Wang, Yibin; Kulkarni, Rajan P.; Nakano, Atsushi; Nguyen, Thao P.; Fei, Peng; Hsiai, Tzung K.

    2017-02-01

    Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the “digital murine heart” to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

  17. Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT.

    Science.gov (United States)

    Hoyer, Patrick; de Medeiros, Gustavo; Balázs, Bálint; Norlin, Nils; Besir, Christina; Hanne, Janina; Kräusslich, Hans-Georg; Engelhardt, Johann; Sahl, Steffen J; Hell, Stefan W; Hufnagel, Lars

    2016-03-29

    We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness around the focal plane. To this end, reversibly switchable fluorophores located right above and below the focal plane are transferred to a nonfluorescent state at each scanning step. LS-RESOLFT nanoscopy offers wide-field 3D imaging of living biological specimens with low light dose and axial resolution far beyond the diffraction barrier. We demonstrate optical sections that are thinner by 5-12-fold compared with their conventional diffraction-limited LS analogs.

  18. Deep Energy Retrofit

    DEFF Research Database (Denmark)

    Zhivov, Alexander; Lohse, Rüdiger; Rose, Jørgen

    Deep Energy Retrofit – A Guide to Achieving Significant Energy User Reduction with Major Renovation Projects contains recommendations for characteristics of some of core technologies and measures that are based on studies conducted by national teams associated with the International Energy Agency...... Energy Conservation in Buildings and Communities Program (IEA-EBC) Annex 61 (Lohse et al. 2016, Case, et al. 2016, Rose et al. 2016, Yao, et al. 2016, Dake 2014, Stankevica et al. 2016, Kiatreungwattana 2014). Results of these studies provided a base for setting minimum requirements to the building...... envelope-related technologies to make Deep Energy Retrofit feasible and, in many situations, cost effective. Use of energy efficiency measures (EEMs) in addition to core technologies bundle and high-efficiency appliances will foster further energy use reduction. This Guide also provides best practice...

  19. Retrofitting models of inflation

    International Nuclear Information System (INIS)

    Kain, Ben

    2009-01-01

    I use the method of retrofitting, developed by Dine, Feng and Silverstein, to generate the scale of inflation dynamically, allowing it to be naturally small. This is a general procedure that may be performed on existing models of supersymmetric inflation. I illustrate this idea on two such models, one an example of F-term inflation and the other an example of D-term inflation.

  20. Studying aerosol light scattering based on aspect ratio distribution observed by fluorescence microscope.

    Science.gov (United States)

    Li, Li; Zheng, Xu; Li, Zhengqiang; Li, Zhanhua; Dubovik, Oleg; Chen, Xingfeng; Wendisch, Manfred

    2017-08-07

    Particle shape is crucial to the properties of light scattered by atmospheric aerosol particles. A method of fluorescence microscopy direct observation was introduced to determine the aspect ratio distribution of aerosol particles. The result is comparable with that of the electron microscopic analysis. The measured aspect ratio distribution has been successfully applied in modeling light scattering and further in simulation of polarization measurements of the sun/sky radiometer. These efforts are expected to improve shape retrieval from skylight polarization by using directly measured aspect ratio distribution.

  1. High-efficiency white organic light-emitting diodes using thermally activated delayed fluorescence

    International Nuclear Information System (INIS)

    Nishide, Jun-ichi; Hiraga, Yasuhide; Nakanotani, Hajime; Adachi, Chihaya

    2014-01-01

    White organic light-emitting diodes (WOLEDs) have attracted much attention recently, aimed for next-generation lighting sources because of their high potential to realize high electroluminescence efficiency, flexibility, and low-cost manufacture. Here, we demonstrate high-efficiency WOLED using red, green, and blue thermally activated delayed fluorescence materials as emissive dopants to generate white electroluminescence. The WOLED has a maximum external quantum efficiency of over 17% with Commission Internationale de l'Eclairage coordinates of (0.30, 0.38).

  2. Retrofit Best Practices Guide

    Energy Technology Data Exchange (ETDEWEB)

    Stovall, T.K.

    2004-01-13

    Few people add siding or change their windows just to reduce their energy bills. But whatever your reasons for retrofitting your home, this will be an important opportunity to improve your home's energy efficiency. Not only will this reduce your utility bills, it will also improve your comfort level and improve our environment. Retrofitting your house is a big deal, and you shouldn't underestimate the effort that will be required to plan the job properly. The energy conservation rewards can be great, but there are also pitfalls that you'll want to avoid. That's what this Best Practices Guide is all about. We can't cover all the issues in these few pages, but we'll tell you some things you need to know if you're changing your siding or windows, and tell you where to learn more about other changes you may want to make to your house. What exactly is a ''best practice''? To put this guide together, we've tested products, talked to contractors and manufacturers, and reviewed the results from a large number of house retrofits. Of course, ''best'' will vary according to the situation. That's why you must start with a careful examination of your house and its existing condition.

  3. Discrimination of Dendrobium officinale and Its Common Adulterants by Combination of Normal Light and Fluorescence Microscopy

    Directory of Open Access Journals (Sweden)

    Chu Chu

    2014-03-01

    Full Text Available The stems of Dendrobium officinale Kimura et Migo, named Tie-pi-shi-hu, is one of the most endangered and precious species in China. Because of its various pharmacodynamic effects, D. officinale is widely recognized as a high-quality health food in China and other countries in south and south-east Asia. With the rising interest of D. officinale, its products have a high price due to a limited supply. This high price has led to the proliferation of adulterants in the market. To ensure the safe use of D. officinale, a fast and convenient method combining normal and fluorescence microscopy was applied in the present study to distinguish D. officinale from three commonly used adulterants including Zi-pi-shi-hu (D. devonianum, Shui-cao-shi-hu (D. aphyllum, Guang-jie-shi-hu (D. gratiosissimum. The result demonstrated that D. officinale could be identified by the characteristic “two hat-shaped” vascular bundle sheath observed under the fluorescence microscopy and the distribution of raphides under normal light microscopy. The other three adulterants could be discriminated by the vascular bundle differences and the distribution of raphides under normal light microscopy. This work indicated that combination of normal light and fluorescence microscopy is a fast and efficient technique to scientifically distinguish D. officinale from the commonly confused species.

  4. Effect of LED photobiomodulation on fluorescent light induced changes in cellular ATPases and Cytochrome c oxidase activity in Wistar rat.

    Science.gov (United States)

    A, Ahamed Basha; C, Mathangi D; R, Shyamala

    2016-12-01

    Fluorescent light exposure at night alters cellular enzyme activities resulting in health defects. Studies have demonstrated that light emitting diode photobiomodulation enhances cellular enzyme activities. The objectives of this study are to evaluate the effects of fluorescent light induced changes in cellular enzymes and to assess the protective role of pre exposure to 670 nm LED in rat model. Male Wistar albino rats were divided into 10 groups of 6 animals each based on duration of exposure (1, 15, and 30 days) and exposure regimen (cage control, exposure to fluorescent light [1800 lx], LED preexposure followed by fluorescent light exposure and only LED exposure). Na + -K + ATPase, Ca 2+ ATPase, and cytochrome c oxidase of the brain, heart, kidney, liver, and skeletal muscle were assayed. Animals of the fluorescent light exposure group showed a significant reduction in Na + -K + ATPase and Ca 2+ ATPase activities in 1 and 15 days and their increase in animals of 30-day group in most of the regions studied. Cytochrome c oxidase showed increase in their level at all the time points assessed in most of the tissues. LED light preexposure showed a significant enhancement in the degree of increase in the enzyme activities in almost all the tissues and at all the time points assessed. This study demonstrates the protective effect of 670 nm LED pre exposure on cellular enzymes against fluorescent light induced change.

  5. Evaluation of Retrofit Delivery Packages

    Energy Technology Data Exchange (ETDEWEB)

    M. Berman, P. Smith, and E. Porse

    2013-07-01

    Residential energy retrofit activities are a critical component of efforts to increase energy efficiency in the U.S. building stock; however, retrofits account for a small percentage of aggregate energy savings at relatively high per unit costs. This report by Building America research team, Alliance for Residential Building Innovation (ARBI), describes barriers to widespread retrofits and evaluates opportunities to improve delivery of home retrofit measures by identifying economies of scale in marketing, energy assessments, and bulk purchasing through pilot programs in portions of Sonoma, Los Angeles, and San Joaquin Counties, CA. These targeted communities show potential and have revealed key strategies for program design, as outlined in the report.

  6. Comparing colour discrimination and proofreading performance under compact fluorescent and halogen lamp lighting.

    Science.gov (United States)

    Mayr, Susanne; Köpper, Maja; Buchner, Axel

    2013-01-01

    Legislation in many countries has banned inefficient household lighting. Consequently, classic incandescent lamps have to be replaced by more efficient alternatives such as halogen and compact fluorescent lamps (CFL). Alternatives differ in their spectral power distributions, implying colour-rendering differences. Participants performed a colour discrimination task - the Farnsworth-Munsell 100 Hue Test--and a proofreading task under CFL or halogen lighting of comparable correlated colour temperatures at low (70 lx) or high (800 lx) illuminance. Illuminance positively affected colour discrimination and proofreading performance, whereas the light source was only relevant for colour discrimination. Discrimination was impaired with CFL lighting. There were no differences between light sources in terms of self-reported physical discomfort and mood state, but the majority of the participants correctly judged halogen lighting to be more appropriate for discriminating colours. The findings hint at the colour-rendering deficiencies associated with energy-efficient CFLs. In order to compare performance under energy-efficient alternatives of classic incandescent lighting, colour discrimination and proofreading performance was compared under CFL and halogen lighting. Colour discrimination was impaired under CFLs, which hints at the practical drawbacks associated with the reduced colour-rendering properties of energy-efficient CFLs.

  7. Removal of fluorescence and ultraviolet absorbance of dissolved organic matter in reclaimed water by solar light.

    Science.gov (United States)

    Wu, Qianyuan; Li, Chao; Wang, Wenlong; He, Tao; Hu, Hongying; Du, Ye; Wang, Ting

    2016-05-01

    Storing reclaimed water in lakes is a widely used method of accommodating changes in the consumption of reclaimed water during wastewater reclamation and reuse. Solar light serves as an important function in degrading pollutants during storage, and its effect on dissolved organic matter (DOM) was investigated in this study. Solar light significantly decreased the UV254 absorbance and fluorescence (FLU) intensity of reclaimed water. However, its effect on the dissolved organic carbon (DOC) value of reclaimed water was very limited. The decrease in the UV254 absorbance intensity and FLU excitation-emission matrix regional integration volume (FLU volume) of reclaimed water during solar light irradiation was fit with pseudo-first order reaction kinetics. The decrease of UV254 absorbance was much slower than that of the FLU volume. Ultraviolet light in solar light had a key role in decreasing the UV254 absorbance and FLU intensity during solar light irradiation. The light fluence-based removal kinetic constants of the UV254 and FLU intensity were independent of light intensity. The peaks of the UV254 absorbance and FLU intensity with an apparent molecular weight (AMW) of 100Da to 2000Da decreased after solar irradiation, whereas the DOC value of the major peaks did not significantly change. Copyright © 2015. Published by Elsevier B.V.

  8. High-intensity light-emitting diode vs fluorescent tubes for intensive phototherapy in neonates.

    Science.gov (United States)

    Sherbiny, Hanan S; Youssef, Doaa M; Sherbini, Ahmad S; El-Behedy, Rabab; Sherief, Laila M

    2016-05-01

    Special blue fluorescent tubes are recommended by the American Academy of Pediatrics (AAP) as the most effective light source for lowering serum bilirubin. A high-intensity light-emitting diode ('super LED') could render intensive phototherapy more effective than the above conventional methods. This study compared the efficacy and safety of a high-intensity light-emitting diode bed vs conventional intensive phototherapy with triple fluorescent tube units as a rescue treatment for severe unconjugated neonatal hyperbilirubinaemia. This was a randomised, prospective trial. Two hundred jaundiced neonates ≥ 35 weeks gestation who met the criteria for intensive phototherapy as per AAP guidelines were randomly assigned to be treated either with triple fluorescent tube units (group 1, n = 100) or a super LED bed (group 2, n = 100). The outcome was the avoidance of exchange transfusion by successful control of hyperbilirubinaemia. Statistically significant higher success rates of intensive phototherapy were achieved among neonates treated with super LED (group 2) than in those treated conventionally (group 1) (87% vs 64%, P = 0.003). Significantly higher 'bilirubin decline' rates were reported in both haemolytic and non-haemolytic subgroups treated with the super LED bed compared with a similar sub-population in the conventionally treated group. Comparable numbers of neonates in both groups developed rebound jaundice (8% vs 10% of groups 1 and 2, respectively). Side-effects were mild in both groups, but higher rates of hyperthermia (12% vs 0%, P = 0.03), dehydration (8% vs 2%, P = 0.26) and skin rash (39% vs 1%, P = 0.002) were reported in the fluorescent tubes-treated group compared with the LED group. Super LED is a safe rescue treatment for severe neonatal hyperbilirubinaemia, and its implementation may reduce the need for exchange transfusion.

  9. Quantitative retinal and choroidal blood flow during light, dark adaptation and flicker light stimulation in rats using fluorescent microspheres.

    Science.gov (United States)

    Shih, Yen-Yu I; Wang, Lin; De La Garza, Bryan H; Li, Guang; Cull, Grant; Kiel, Jeffery W; Duong, Timothy Q

    2013-02-01

    The present study aimed to quantify retinal and choroidal blood flow (BF) during light, dark adaptation and flicker light stimulation using the microsphere technique. Adult male Sprague-Dawley rats were anesthetized with isoflurane. Eyes were dark (Group I, n = 8), light (Group II, n = 8) adapted or stimulated with 10 Hz flicker light (Group III, n = 10). Retinal and choroidal BF were measured by a previously established method, using a mixture of 8 µm yellow-green and 10 µm red fluorescent microspheres. The microspheres were counted ex vivo in the dissected retina and choroid and in the reference arterial blood under a fluorescent microscope. The choroidal BF was 64.8 ± 29 µl/min (mean ± SD) during dark adaptation, not significantly different from that during light adaptation (66.0 ± 17.8 µl/min). The retinal BF was 13.5 ± 3.2 µl/min during 10 Hz flickering light stimulation, significantly higher than that during dark adaptation in the control fellow eyes (9.9 ± 2.9 µl/min). The choroidal BF values were not statistically different between flicker stimulation and dark adaptation. Retinal BF was 11.6 ± 2.9 µl/min during light adaptation. Dark adaptation did not increase retinal BF (Group I, 8.2 ± 2.4 µl/min; Group II, 9.9 ± 2.9 µl/min). These findings argue against a dark-induced or flicker-induced functional hyperemia in the choroid as a result of the demands of the outer retina. Retinal BF was not higher during dark adaptation. Our data support the conclusion that the inner retina has a higher energy demand in flicker conditions relative to dark.

  10. Differential responses of seven contrasting species to high light using pigment and chlorophyll a fluorescence

    Directory of Open Access Journals (Sweden)

    Mittal S.

    2011-05-01

    Full Text Available High light intensity may induce severe photodamage to chloroplast and consequently cause decreases in the yield capacity of plants and destruction of pigments, causing an overall yellowing of the foliage. Thus, study related to light adaptation becomes necessary to understand adaptation processes in higher plants on the basis of which they are characterized as full sunlight or shade plants. Chlorophyll can be regarded as an intrinsic fluorescent probe of the photosynthetic system. The ecophysiological parameter related to plant performance and fitness i.e. in-situ chlorophyll fluorescence measurements were determined for different plant species in the medicinal plant garden of Banasthali University, Rajasthan. Miniaturized Pulse Amplitude Modulated Photosynthetic Yield Analyzers are primarily designed for measuring effective quantum yield (ΔF/Fm’ of photosystem II under momentary ambient light in the field. Photosynthetic yield measurements and light-response curves suggested a gradation of sun-adapted to shade-adapted behaviour of these plants in following order Withania somnifera> Catharanthus roseus> Datura stamonium> Vasica minora> Vasica adulta> Rauwolfia serpentina. As indicated by light response curves and pigment analysis, Datura stramonium, Withania somnifera and Catharanthus roseus competed well photosynthetically and are favoured while Rauwolfia serpentina, Vasica minora, Vasica adulta and Plumbago zeylanica were observed to be less competent photosynthetically. These light response curves and resultant cardinal points study gave insight into the ecophysiological characterization of the photosynthetic capacity of the plant and provides highly interesting parameters like electron transport rate, photo-inhibition, photosynthetically active photon flux density and yield on the basis of which light adaptability was screened for seven medicinally important plants.

  11. Development of a High Output Fluorescent Light Module for the Commercial Plant Biotechnology Facility

    Science.gov (United States)

    Turner, Mark; Zhou, Wei-Jia; Doty, Laura (Technical Monitor)

    2000-01-01

    To maximize the use of available resources provided onboard the International Space Station, the development of an efficient lighting 1 system is critical to the overall performance of the CPBF. Not only is it important to efficiently generate photon energy, but thermal loads on the CPBF Temperature and Humidity Control System must be minimized. By utilizing optical coatings designed to produce highly diffuse reflectance in the visible wavelengths while minimizing reflectance in the infrared region, the design of the fluorescent light module for the CPBF is optimized for maximum photon flux, spatial uniformity and energy efficiency. Since the Fluorescent Light Module must be fully enclosed to meet (ISS) requirements for containment of particulates and toxic materials, heat removal from the lights presented some unique design challenges. By using the Express Rack moderate C, temperature-cooling loop, heat is rejected by means of a liquid/air coolant manifold. Heat transfer to the manifold is performed by conduction using copper fins, by forced air convection using miniature fans, and by radiation using optically selective coatings that absorb in the infrared wavelengths. Using this combination of heat transfer mechanisms builds in redundancy to prevent thermal build up and premature bulb failure.

  12. Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis.

    Science.gov (United States)

    Bhalla, Manpreet; Sidiq, Zeeshan; Sharma, P P; Singhal, Ritu; Myneedu, V P; Sarin, Rohit

    2013-09-01

    Fluorescence microscopy (FM) over the years has shown the potential for increasing the performance of microscopy. The present study was aimed to access the performance of the LED microscope for the detection of acid fast bacilli in a tuberculosis (TB) endemic country. The study was conducted at a National Reference Laboratory (NRL) in New Delhi, India. Sputum samples were collected from suspected TB patients. Each sample was processed with Auramine O and ZN methods. Auramine O stained smears were evaluated using two different excitatory light sources (MVP and LED); and ZN stained smears were examined under light microscope. The mean time required to read the smears with different modalities was recorded. Bacterial cultures provided the reference standard. A total of 200 patients were included in this study. Sensitivity and specificity for the LED assessment, MVP assessment and light microscopy were 83.1% and 82.4%, 78.5% and 87.5% and 81.6% and 83.5%, respectively. Mean reading time was approximately three times faster than ZN microscopy. The mean time to read a negative smear was 2min with fluorescence microscopy and 5min with light microscopy with time savings of 60%. Although the use of LED-FM only marginally increased sensitivity, the considerable time saving ability combined with very good acceptance and ease of use makes it a reliable alternative to other conventional methods available. Copyright © 2013 Asian-African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

  13. On the polyphasic quenching kinetics of chlorophyll a fluorescence in algae after light pulses of variable length

    NARCIS (Netherlands)

    Vredenberg, W.J.; Prasil, O.

    2013-01-01

    This study reports on kinetics of the fluorescence decay in a suspension of the alga Scenedesmus quadricauda after actinic illumination. These are monitored as the variable fluorescence signal in the dark following light pulses of variable intensity and duration. The decay reflects the restoration

  14. TEMPERATURE-DEPENDENCE OF CHLOROPHYLL FLUORESCENCE INDUCTION AND PHOTOSYNTHESIS IN TOMATO AS AFFECTED BY TEMPERATURE AND LIGHT CONDITIONS DURING GROWTH

    NARCIS (Netherlands)

    JANSSEN, LHJ; WAMS, HE; VANHASSELT, PR

    The temperature dependence of chlorophyll fluorescence induction and photosynthesis of tomato plants grown at different temperatures and light intensities was studied. Chlorophyll fluorescence induction and photosynthetic activity of leaf discs was determined between 0-degrees and 30-degrees-C. Two

  15. Ambient light effect on the uniformity of image intensifier fluorescence screen brightness

    Science.gov (United States)

    Qiu, YaFeng; Chang, BenKang; Sun, LianJun; Zhang, JunJu; Gao, YouTang; Fu, RongGuo

    2008-02-01

    When testing the uniformity of Image intensifier fluorescence screen brightness, the million scale CCD brightness meter is used. Due to the distance between the meter and fluorescence screen, the effect of ambient light on the testing result is essential to the design of testing system. Test with super second generation tube, input a constant voltage to insure the fluorescence screen brightness to be constant. Collect the brightness of the same fluorescence screen in different ambient luminance environment of 1×102Lx, 1×101Lx, 1Lx, 1×10-1Lx, 1×10-2Lx, 1×10-3Lx. Study the results with software MATLAB. It is concluded as: In ambient luminance environment of 1×10-1Lx the CCD has the best result. The testing result in ambient luminance environment of above 1×103Lx show untrue image. The testing result in ambient luminance environment of below 1×10-3Lx shows its own noise image and is unbelievable either.

  16. A combined light sheet fluorescence and differential interference contrast microscope for live imaging of multicellular specimens.

    Science.gov (United States)

    Baker, R P; Taormina, M J; Jemielita, M; Parthasarathy, R

    2015-05-01

    We describe a microscope capable of both light sheet fluorescence microscopy and differential interference contrast microscopy (DICM). The two imaging modes, which to the best of our knowledge have not previously been combined, are complementary: light sheet fluorescence microscopy provides three-dimensional imaging of fluorescently labelled components of multicellular systems with high speed, large fields of view, and low phototoxicity, whereas differential interference contrast microscopy reveals the unlabelled neighbourhood of tissues, organs, and other structures with high contrast and inherent optical sectioning. Use of a single Nomarski prism for differential interference contrast microscopy and a shared detection path for both imaging modes enables simple integration of the two techniques in one custom microscope. We provide several examples of the utility of the resulting instrument, focusing especially on the digestive tract of the larval zebrafish, revealing in this complex and heterogeneous environment anatomical features, the behaviour of commensal microbes, immune cell motions, and more. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

  17. Low light CMOS contact imager with an integrated poly-acrylic emission filter for fluorescence detection.

    Science.gov (United States)

    Dattner, Yonathan; Yadid-Pecht, Orly

    2010-01-01

    This study presents the fabrication of a low cost poly-acrylic acid (PAA) based emission filter integrated with a low light CMOS contact imager for fluorescence detection. The process involves the use of PAA as an adhesive for the emission filter. The poly-acrylic solution was chosen due its optical transparent properties, adhesive properties, miscibility with polar protic solvents and most importantly its bio-compatibility with a biological environment. The emission filter, also known as an absorption filter, involves dissolving an absorbing specimen in a polar protic solvent and mixing it with the PAA to uniformly bond the absorbing specimen and harden the filter. The PAA is optically transparent in solid form and therefore does not contribute to the absorbance of light in the visible spectrum. Many combinations of absorbing specimen and polar protic solvents can be derived, yielding different filter characteristics in different parts of the spectrum. We report a specific combination as a first example of implementation of our technology. The filter reported has excitation in the green spectrum and emission in the red spectrum, utilizing the increased quantum efficiency of the photo sensitive sensor array. The thickness of the filter (20 μm) was chosen by calculating the desired SNR using Beer-Lambert's law for liquids, Quantum Yield of the fluorophore and the Quantum Efficiency of the sensor array. The filters promising characteristics make it suitable for low light fluorescence detection. The filter was integrated with a fully functional low noise, low light CMOS contact imager and experimental results using fluorescence polystyrene micro-spheres are presented.

  18. An LED light source and novel fluorophore combinations improve fluorescence laparoscopic detection of metastatic pancreatic cancer in orthotopic mouse models.

    Science.gov (United States)

    Metildi, Cristina A; Kaushal, Sharmeela; Lee, Claudia; Hardamon, Chanae R; Snyder, Cynthia S; Luiken, George A; Talamini, Mark A; Hoffman, Robert M; Bouvet, Michael

    2012-06-01

    The aim of this study was to improve fluorescence laparoscopy of pancreatic cancer in an orthotopic mouse model with the use of a light-emitting diode (LED) light source and optimal fluorophore combinations. Human pancreatic cancer models were established with fluorescent FG-RFP, MiaPaca2-GFP, BxPC-3-RFP, and BxPC-3 cancer cells implanted in 6-week-old female athymic mice. Two weeks postimplantation, diagnostic laparoscopy was performed with a Stryker L9000 LED light source or a Stryker X8000 xenon light source 24 hours after tail-vein injection of CEA antibodies conjugated with Alexa 488 or Alexa 555. Cancer lesions were detected and localized under each light mode. Intravital images were also obtained with the OV-100 Olympus and Maestro CRI Small Animal Imaging Systems, serving as a positive control. Tumors were collected for histologic analysis. Fluorescence laparoscopy with a 495-nm emission filter and an LED light source enabled real-time visualization of the fluorescence-labeled tumor deposits in the peritoneal cavity. The simultaneous use of different fluorophores (Alexa 488 and Alexa 555), conjugated to antibodies, brightened the fluorescence signal, enhancing detection of submillimeter lesions without compromising background illumination. Adjustments to the LED light source permitted simultaneous detection of tumor lesions of different fluorescent colors and surrounding structures with minimal autofluorescence. Using an LED light source with adjustments to the red, blue, and green wavelengths, it is possible to simultaneously identify tumor metastases expressing fluorescent proteins of different wavelengths, which greatly enhanced the signal without compromising background illumination. Development of this fluorescence laparoscopy technology for clinical use can improve staging and resection of pancreatic cancer. Copyright © 2012 American College of Surgeons. Published by Elsevier Inc. All rights reserved.

  19. Retrofitting Systems for External Walls

    DEFF Research Database (Denmark)

    Rose, Jørgen

    1997-01-01

    In this report, 9 different external and internal retrofitting systems are analyzed using numerical calculations. The analysis focuses on the thermal bridge effects in the different systems, and on this basis it is discussed whether internal or external retrofitting has the most advantages. The d....... The different systems are evaluated using 5 different types of existing walls....

  20. Quantitative light fluorescence (QLF) and polarized white light (PWL) assessments of dental fluorosis in an epidemiological setting.

    Science.gov (United States)

    Pretty, Iain A; McGrady, Michael; Zakian, Christian; Ellwood, Roger P; Taylor, Andrew; Sharif, Mohammed Owaise; Iafolla, Timothy; Martinez-Mier, E Angeles; Srisilapanan, Patcharawan; Korwanich, Narumanas; Goodwin, Michaela; Dye, Bruce A

    2012-05-20

    To determine if a novel dual camera imaging system employing both polarized white light (PWL) and quantitative light induced fluorescence imaging (QLF) is appropriate for measuring enamel fluorosis in an epidemiological setting. The use of remote and objective scoring systems is of importance in fluorosis assessments due to the potential risk of examiner bias using clinical methods. Subjects were recruited from a panel previously characterized for fluorosis and caries to ensure a range of fluorosis presentation. A total of 164 children, aged 11 years (±1.3) participated following consent. Each child was examined using the novel imaging system, a traditional digital SLR camera, and clinically using the Dean's and Thylstrup and Fejerskov (TF) Indices on the upper central and lateral incisors. Polarized white light and SLR images were scored for both Dean's and TF indices by raters and fluorescence images were automatically scored using software. Data from 164 children were available with a good distribution of fluorosis severity. The automated software analysis of QLF images demonstrated significant correlations with the clinical examinations for both Dean's and TF index. Agreement (measured by weighted Kappa's) between examiners scoring clinically, from polarized photographs and from SLR images ranged from 0.56 to 0.92. The study suggests that the use of a digital imaging system to capture images for either automated software analysis, or remote assessment by raters is suitable for epidemiological work. The use of recorded images enables study archiving, assessment by multiple examiners, remote assessment and objectivity due to the blinding of subject status.

  1. Quantitative Light Fluorescence (QLF and Polarized White Light (PWL assessments of dental fluorosis in an epidemiological setting

    Directory of Open Access Journals (Sweden)

    Pretty Iain A

    2012-05-01

    Full Text Available Abstract Background To determine if a novel dual camera imaging system employing both polarized white light (PWL and quantitative light induced fluorescence imaging (QLF is appropriate for measuring enamel fluorosis in an epidemiological setting. The use of remote and objective scoring systems is of importance in fluorosis assessments due to the potential risk of examiner bias using clinical methods. Methods Subjects were recruited from a panel previously characterized for fluorosis and caries to ensure a range of fluorosis presentation. A total of 164 children, aged 11 years (±1.3 participated following consent. Each child was examined using the novel imaging system, a traditional digital SLR camera, and clinically using the Dean’s and Thylstrup and Fejerskov (TF Indices on the upper central and lateral incisors. Polarized white light and SLR images were scored for both Dean’s and TF indices by raters and fluorescence images were automatically scored using software. Results Data from 164 children were available with a good distribution of fluorosis severity. The automated software analysis of QLF images demonstrated significant correlations with the clinical examinations for both Dean’s and TF index. Agreement (measured by weighted Kappa’s between examiners scoring clinically, from polarized photographs and from SLR images ranged from 0.56 to 0.92. Conclusions The study suggests that the use of a digital imaging system to capture images for either automated software analysis, or remote assessment by raters is suitable for epidemiological work. The use of recorded images enables study archiving, assessment by multiple examiners, remote assessment and objectivity due to the blinding of subject status.

  2. 3D fluorescence emission difference microscopy based on spatial light modulator

    Directory of Open Access Journals (Sweden)

    Guangyuan Zhao

    2016-05-01

    Full Text Available We report three-dimensional fluorescence emission difference (3D-FED microscopy using a spatial light modulator (SLM. Zero phase, 0–2π vortex phase and binary 0-pi phase are loaded on the SLM to generate the corresponding solid, doughnut and z-axis hollow excitation spot, respectively. Our technique achieves super-resolved image by subtracting three differently acquired images with proper subtractive factors. Detailed theoretical analysis and simulation tests are proceeded to testify the performance of 3D-FED. Also, the improvement of lateral and axial resolution is demonstrated by imaging 100nm fluorescent beads. The experiment yields lateral resolution of 140nm and axial resolution of approximate 380nm.

  3. Emergence of White Organic Light-Emitting Diodes Based on Thermally Activated Delayed Fluorescence

    Directory of Open Access Journals (Sweden)

    Peng Xiao

    2018-02-01

    Full Text Available Recently, thermally activated delayed fluorescence (TADF organic light-emitting diodes (OLEDs have attracted both academic and industrial interest due to their extraordinary characteristics, such as high efficiency, low driving voltage, bright luminance, lower power consumption and potentially long lifetime. In this invited review, the fundamental concepts of TADF have been firstly introduced. Then, main approaches to realize WOLEDs based on TADF have been summarized. More specifically, the recent development of WOLEDs based on all TADF emitters, WOLEDs based on TADF and conventional fluorescence emitters, hybrid WOLEDs based on blue TADF and phosphorescence emitters and WOLEDs based on TADF exciplex host and phosphorescence dopants is highlighted. In particular, design strategies, device structures, working mechanisms and electroluminescent processes of the representative WOLEDs based on TADF are reviewed. Finally, challenges and opportunities for further enhancement of the performance of WOLEDs based on TADF are presented.

  4. Spectral signatures of fluorescence and light absorption to identify crude oils found in the marine environment

    Science.gov (United States)

    Baszanowska, E.; Otremba, Z.

    2014-08-01

    To protect the natural marine ecosystem, it is necessary to continuously enhance knowledge of environmental contamination, including oil pollution. Therefore, to properly track the qualitative and quantitative changes in the natural components of seawater, a description of the essential spectral features describing petroleum products is necessary. This study characterises two optically-different types of crude oils (Petrobaltic and Romashkino) - substances belonging to multi-fluorophoric systems. To obtain the spectral features of crude oils, the excitation-emission spectroscopy technique was applied. The fluorescence and light absorption properties for various concentrations of oils at a stabilised temperature are described. Both excitation-emission spectra (EEMs) and absorption spectra of crude oils are discussed. Based on the EEM spectra, both excitation end emission peaks for the wavelengthindependent fluorescence maximum (Exmax/ Emmax) - characteristic points for each type of oil - were identified and compared with the literature data concerning typical marine chemical structures.

  5. Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron microscopy.

    Science.gov (United States)

    Faas, F G A; Bárcena, M; Agronskaia, A V; Gerritsen, H C; Moscicka, K B; Diebolder, C A; van Driel, L F; Limpens, R W A L; Bos, E; Ravelli, R B G; Koning, R I; Koster, A J

    2013-03-01

    Correlative light and electron microscopy is an increasingly popular technique to study complex biological systems at various levels of resolution. Fluorescence microscopy can be employed to scan large areas to localize regions of interest which are then analyzed by electron microscopy to obtain morphological and structural information from a selected field of view at nm-scale resolution. Previously, an integrated approach to room temperature correlative microscopy was described. Combined use of light and electron microscopy within one instrument greatly simplifies sample handling, avoids cumbersome experimental overheads, simplifies navigation between the two modalities, and improves the success rate of image correlation. Here, an integrated approach for correlative microscopy under cryogenic conditions is presented. Its advantages over the room temperature approach include safeguarding the native hydrated state of the biological specimen, preservation of the fluorescence signal without risk of quenching due to heavy atom stains, and reduced photo bleaching. The potential of cryo integrated light and electron microscopy is demonstrated for the detection of viable bacteria, the study of in vitro polymerized microtubules, the localization of mitochondria in mouse embryonic fibroblasts, and for a search into virus-induced intracellular membrane modifications within mammalian cells. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. Post-Retrofit Residential Assessments

    Energy Technology Data Exchange (ETDEWEB)

    Lancaster, Ross; lutzenhiser, Loren; Moezzi, Mithra; Widder, Sarah H.; Chandra, Subrato; Baechler, Michael C.

    2012-04-30

    This study examined a range of factors influencing energy consumption in households that had participated in residential energy-efficiency upgrades. The study was funded by a grant from the U.S. Department of Energy’s Pacific Northwest National Laboratory and was conducted by faculty and staff of Portland State University Center for Urban Studies and Department of Economics. This work was made possible through the assistance and support of the Energy Trust of Oregon (ETO), whose residential energy-efficiency programs provided the population from which the sample cases were drawn. All households in the study had participated in the ETO Home Performance with Energy Star (HPwES) program. A number of these had concurrently pursued measures through other ETO programs. Post-retrofit energy outcomes are rarely investigated on a house-by-house basis. Rather, aggregate changes are ordinarily the focus of program impact evaluations, with deviation from aggregate expectations chalked up to measurement error, the vagaries of weather and idiosyncrasies of occupants. However, understanding how homes perform post-retrofit on an individual basis can give important insights to increase energy savings at the participant and the programmatic level. Taking a more disaggregated approach, this study analyzed energy consumption data from before and after the retrofit activity and made comparisons with engineering estimates for the upgrades, to identify households that performed differently from what may have been expected based on the estimates. A statistical analysis using hierarchal linear models, which accounted for weather variations, was performed looking separately at gas and electrical use during the periods before and after upgrades took place. A more straightforward comparison of billing data for 12-month periods before and after the intervention was also performed, yielding the majority of the cases examined. The later approach allowed total energy use and costs to be

  7. Fluorescence imaging under background light with a self-reset complementary metal–oxide–semiconductor image sensor

    Directory of Open Access Journals (Sweden)

    Takahiro Yamaguchi

    2015-11-01

    Full Text Available The authors propose and demonstrate the fluorescence imaging of green fluorescence protein (GFP expressed in a brain slice with a self-reset complementary metal–oxide–semiconductor image sensor under background light. By using a self-reset function to avoid pixel saturation, the weak fluorescence of GFP was successfully observed, even under background light. The result suggests that the sensor can be applied to in vivo imaging of laboratory animals during light–dark cycles, so that they can observe the different responses to bright and dark environments.

  8. Integrated OLED as excitation light source in fluorescent lateral flow immunoassays.

    Science.gov (United States)

    Venkatraman, Vishak; Steckl, Andrew J

    2015-12-15

    The integration of organic light emitting diodes (OLEDs) as excitation light sources for quantum dot-based fluorescent lateral flow immunoassay systems (LFIA) was investigated. This approach has the potential to deliver a sensitive visible detection scheme for low-cost, disposable lab-on-chip point-of-care (POC) diagnosis system. Thin film phosphorescent green OLEDs fabricated on plastic substrates were integrated on-chip to excite the test line of a quantum dot-based LFIA (QD-LFIA). OLEDs were fabricated by sequential deposition of organic thin films (total of ~100 nm) onto ITO-coated PET substrates. CdSe/ZnS QDs emitting at 655 nm and Au nanoparticles (NP - 10 nm size) conjugated antibodies were used for the fluorescence QD-LFIA and conventional reflection-mode Au NP-LFIA, respectively. Thin plastic color light filters were integrated for filtering the excitation light source and, thereby, increasing the contrast of the emitted light for optimized visual detection. Integration of the OLED and color filters with the analytical membrane was achieved using adhesive techniques facilitated by the planar nature of the layers, which suggests possible large scale manufacturing using roll-to-roll processing. Gray scale analysis from digital images captured with a digital camera was used to quantify the visual sensitivity. The signal intensity, signal-to-noise ratio (SNR) and the limit of detection (LOD) of OLED integrated QD-LFIAs were compared to Au NP LFIAs. OLED QD-LFIA exhibited superior performance in all signal aspects: 7-8× higher signal intensity and SNR, and a 7× lower LOD of 3 nM (measured at S/N=3). These results demonstrate the potential of OLED-integrated in LFIA devices for obtaining sensitive, fast and low-cost POC diagnostics. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Obstacles and opportunities in the commercialization of the solid-state-electronic fluorescent-lighting ballast

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, D.R.; Marcus, A.A.; Campbell, R.S.; Sommers, P.; Skumatz, L.; Berk, B.; Petty, P.; Eschbach, C.

    1981-10-01

    The Solid State Ballast (SSB) Program, aimed at improving the efficiency of fluorescent lights, is described. The first generation of solid state electronic ballasts has been developed and the technology has been transferred to the private sector. This report examines the opportunities for rapid dissemination of this technology into the marketplace. It includes a description of product characteristics and their influence on the commercialization of the SSB, a description of the technology delivery system presently used by the ballast industry, an analysis of the market for SSB, and identification of some high-leverage opportunities to accelerate the commercialization process. (MCW)

  10. Irradiance Decay in Fluorescent and Light-emitting Diode-based Phototherapy Devices: A Pilot Study.

    OpenAIRE

    Olusanya, Bolajoko

    2016-01-01

    We set out to determine the rate of decline of irradiance for fluorescent tube (FT) and light-emitting diode (LED) phototherapy devices in resource-limited settings where routine irradiance monitoring is uncommon. Irradiance levels (μW/cm 2 /nm) were measured weekly using BiliBlanket ® II Meter on three FT-based and two LED-based phototherapy devices over a 19 week period. The two LED devices showed stable irradiance levels and did not require any lamp changes. The three FT-based devices show...

  11. New fluorescent dipolar pyrazine derivatives for non-doped red organic light-emitting diodes

    International Nuclear Information System (INIS)

    Gao Baoxiang; Zhou Quanguo; Geng Yanhou; Cheng Yanxiang; Ma Dongge; Xie Zhiyuan; Wang Lixiang; Wang Fosong

    2006-01-01

    Dipolar fluorescent compounds containing electron-accepting pyrazine-2,3-dicarbonitrile and electron-donating arylamine moiety have been designed and synthesized. The optical and electrochemical properties of these compounds can be adjusted by changing π-bridge length and the donor (D) strength. Organic light-emitting devices based on these compounds are fabricated. Saturated red emission of (0.67, 0.33) and the external quantum efficiency as high as 1.41% have been demonstrated for one of these compounds

  12. Compact Fluorescent Lighting in America: Lessons Learned on the Way to Market

    Energy Technology Data Exchange (ETDEWEB)

    Sandahl, Linda J.; Gilbride, Theresa L.; Ledbetter, Marc R.; Steward, Heidi E.; Calwell, Chris

    2006-05-22

    This report describes the history of compact fluorescent lamps (CFLs) in America. CFLs were introduced in the 1970s; however, it has taken more than 20 years for them to gain widespread recognition in the U.S. residential lighting market. This report reviews the development of CFLs, efforts to increase market acceptance of them, and barriers to that acceptance. Lessons to be learned from this study of CFLs are identified in hopes of assisting future market introduction efforts for other promising energy-efficient technologies. This report was prepared by the Pacific Northwest National Laboratory for the U.S. Department of Energy’s Office of Building Technologies, Emerging Technologies Program.

  13. Effect of light-emitting diode vs. fluorescent lighting on laying hens in aviary hen houses: Part 1 - Operational characteristics of lights and production traits of hens.

    Science.gov (United States)

    Long, H; Zhao, Y; Wang, T; Ning, Z; Xin, H

    2016-01-01

    Light-emitting diode (LED) lights are becoming more affordable for agricultural applications. Despite many lab-scale studies concerning impact of LED on poultry, little research has been documented under field production conditions, especially for laying hens. This 15-month field study was carried out to evaluate the effects of LED vs. fluorescent (FL) lights on laying hens (Dekalb white breed) using 4 (2 pairs) aviary hen houses each at a nominal capacity of 50,000 hens. The evaluation was done regarding operational characteristics of the lights and hen production traits. The results show that spatial distribution of the LED light was less uniform than that of the FL light. Light intensity of the LED light decreased by 27% after 3,360 h use but remained quite steady from 3,360 to 5,760 h use. Eleven out of 762 (1.44%) LED lamps (new at onset of the study) in the 2 houses failed during the 15-month experiment period. The neck area of the LED lamp was hottest, presumably the primary reason for the lamp failure as cracks were noticed in the neck region of all failed LED lamps. No differences were observed in egg weight, hen-day egg production, feed use, and mortality rate between LED and FL regimens. However, hens under the FL had higher eggs per hen housed and better feed conversion than those under the LED during 20 to 70 wk production (P Hens under the LED tended to have less feather uniformity and insulation than those under the FL (P hens under the LED showed a larger median avoidance distance than those under the FL at 36 wk age (P hens under the LED were more alert; but no difference at 60 wk age. More comparative research to quantify behavioral and production responses of different breeds of hens to LED vs. FL lighting seems warranted. © 2015 Poultry Science Association Inc.

  14. Developing a genetically encoded green fluorescent protein mutant for sensitive light-up fluorescent sensing and cellular imaging of Hg(II).

    Science.gov (United States)

    Jiang, Tao; Guo, Daiping; Wang, Qian; Wu, Xin; Li, Zhao; Zheng, Zhenhua; Yin, Boyuan; Xia, Lin; Tang, Jixian; Luo, Wenxin; Xia, Ningshao; Jiang, Yunbao

    2015-05-30

    Hg(II) is well-known for quenching fluorescence in a distance dependent manner. Nevertheless, when we exposed the fluorophore of a green fluorescent protein (GFP) toward Hg(II), through H148C mutation, the GFP fluorescence could be "lighted up" by Hg(II) down to sub-nM level. The detection linear range is 0.5-3.0 nM for protein solutions at 8.0 nM. The GFPH148C protein displayed a promising selectivity toward Hg(II) and also the cellular imaging capacity. Spectra measurements suggested that the ground-state redistribution of protein contributed to the fluorescence enhancement, which was found not limited to Hg(II), and thus presented an opening for building a pool of GFP-based chemosensors toward other heavy metal ions. Copyright © 2015. Published by Elsevier B.V.

  15. UV-A emission from fluorescent energy-saving light bulbs alters local retinoic acid homeostasis.

    Science.gov (United States)

    Hellmann-Regen, Julian; Heuser, Isabella; Regen, Francesca

    2013-12-01

    Worldwide bans on incandescent light bulbs (ILBs) drive the use of compact fluorescent light (CFL) bulbs, which emit ultraviolet (UV) radiation. Potential health issues of these light sources have already been discussed, including speculation about the putative biological effects on light exposed tissues, yet the underlying mechanisms remain unclear. We hypothesized photoisomerization of all-trans retinoic acid (at-RA), a highly light sensitive morphogen, into biologically less active isomers, as a mechanism mediating biological effects of CFLs. Local at-RA is anti-carcinogenic, entrains molecular rhythms and is crucial for skin homeostasis. Therefore, we quantified the impact of CFL irradiation on extra- and intracellular levels of RA isomers using an epidermal cell culture model. Moreover, a biologically relevant impact of CFL irradiation was assessed using highly at-RA-sensitive human neuroblastoma cells. Dose-dependent conversion of extra- and intracellular at-RA into the biologically less active 13-cis-isomer was significantly higher in CFL vs. ILB exposure and completely preventable by employing a UV-filter. Moreover, pre-irradiation of culture media by CFL attenuated at-RA-specific effects on cell viability in human at-RA-sensitive cells in a dose-dependent manner. These findings point towards a biological relevance of CFL-induced at-RA decomposition, providing a mechanism for CFL-mediated effects on environmental health.

  16. The health risks associated with energy efficient fluorescent, LEDs, and artificial lighting

    Science.gov (United States)

    Panahi, Allen

    2014-09-01

    With the phasing out of incandescent lamps in many countries, the introduction of new LED based light sources and luminaries sometimes raise the question of whether the spectral characteristics of the LED and other energy savings Fluorescent lights including the popular CFLs are suitable to replace the traditional incandescent lamps. These concerns are sometimes raised particularly for radiation emissions in the UV and Blue parts of the spectrum. This paper aims to address such concerns for the common `white light' sources typically used in household and other general lighting used in the work place. Recent studies have shown that women working the night shift have an increased probability of developing breast cancer. We like to report on the findings of many studies done by medical professionals, in particular the recent announcement of AMA in the US and many studies conducted in the UK, as well as the European community to increase public awareness on the long term health risks of the optical and opto-biological effects on the human health caused by artificial lighting.

  17. Performance of a Hot-Dry Climate Whole-House Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Weitzel, E.; German, A.; Porse, E.

    2014-06-01

    The Stockton house retrofit is a two-story tudor style single family deep retrofit in the hot-dry climate of Stockton, CA. The home is representative of a deep retrofit option of the scaled home energy upgrade packages offered to targeted neighborhoods under the pilot Large-Scale Retrofit Program (LSRP) administered by the Alliance for Residential Building Innovation (ARBI). Deep retrofit packages expand on the standard package by adding HVAC, water heater and window upgrades to the ducting, attic and floor insulation, domestic hot water insulation, envelope sealing, lighting and ventilation upgrades. Site energy savings with the deep retrofit were 23% compared to the pre-retrofit case, and 15% higher than the savings estimated for the standard retrofit package. Energy savings were largely a result of the water heater upgrade, and a combination of the envelope sealing, insulation and HVAC upgrade. The HVAC system was of higher efficiency than the building code standard. Overall the financed retrofit would have been more cost effective had a less expensive HVAC system been selected and barriers to wall insulation remedied. The homeowner experienced improved comfort throughout the monitored period and was satisfied with the resulting utility bill savings.

  18. Performance of a Hot-Dry Climate Whole-House Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Weitzel, E. [Alliance for Residential Building Innovation, Davis, CA (United States); German, A. [Alliance for Residential Building Innovation, Davis, CA (United States); Porse, E. [Alliance for Residential Building Innovation, Davis, CA (United States)

    2014-06-01

    The Stockton house retrofit is a two-story Tudor style single family deep retrofit in the hot-dry climate of Stockton, CA. The home is representative of a deep retrofit option of the scaled home energy upgrade packages offered to targeted neighborhoods under the pilot Large-Scale Retrofit Program (LSRP) administered by the Alliance for Residential Building Innovation (ARBI). Deep retrofit packages expand on the standard package by adding HVAC, water heater and window upgrades to the ducting, attic and floor insulation, domestic hot water insulation, envelope sealing, lighting and ventilation upgrades. Site energy savings with the deep retrofit were 23% compared to the pre-retrofit case, and 15% higher than the savings estimated for the standard retrofit package. Energy savings were largely a result of the water heater upgrade, and a combination of the envelope sealing, insulation and HVAC upgrade. The HVAC system was of higher efficiency than the building code standard. Overall the financed retrofit would have been more cost effective had a less expensive HVAC system been selected and barriers to wall insulation remedied. The homeowner experienced improved comfort throughout the monitored period and was satisfied with the resulting utility bill savings.

  19. Short-Term Test Results: Transitional Housing Energy Efficiency Retrofit in the Hot-Humid Climate

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K.; Martin, E.

    2013-02-01

    This project evaluates the renovation of a 5,800 ft2, multi-use facility located in St. Petersburg, on the west coast of central Florida, in the hot humid climate. An optimal package of retrofit measures was designed to deliver 30-40% annual energy cost savings for this building with annual utility bills exceeding $16,000 and high base load consumption. Researchers projected energy cost savings for potential retrofit measures based on pre-retrofit findings and disaggregated, weather normalized utility bills as a basis for simulation true-up. A cost-benefit analysis was conducted for the seven retrofit measures implemented; adding attic insulation and sealing soffits, tinting windows, improving whole building air-tightness, upgrading heating and cooling systems and retrofitting the air distribution system, replacing water heating systems, retrofitting lighting, and replacing laundry equipment. The projected energy cost savings for the full retrofit package based on a post-retrofit audit is 35%. The building's architectural characteristics, vintage, and residential and commercial uses presented challenges for both economic projections and retrofit measure construction.

  20. Short-Term Test Results. Transitional Housing Energy Efficiency Retrofit in the Hot Humid Climate

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [BA-PIRC/Florida Solar Energy Center, Cocoa, FL (United States); Martin, Eric [BA-PIRC/Florida Solar Energy Center, Cocoa, FL (United States)

    2013-02-01

    This project evaluates the renovation of a 5,800 ft2, multi-use facility located in St. Petersburg, on the west coast of central Florida, in the hot humid climate. An optimal package of retrofit measures was designed to deliver 30%-40% annual energy cost savings for this building with annual utility bills exceeding $16,000 and high base load consumption. Researchers projected energy cost savings for potential retrofit measures based on pre-retrofit findings and disaggregated, weather normalized utility bills as a basis for simulation true-up. A cost-benefit analysis was conducted for the seven retrofit measures implemented; adding attic insulation and sealing soffits, tinting windows, improving whole building air-tightness, upgrading heating and cooling systems and retrofitting the air distribution system, replacing water heating systems, retrofitting lighting, and replacing laundry equipment. The projected energy cost savings for the full retrofit package based on a post-retrofit audit is 35%. The building's architectural characteristics, vintage, and residential and commercial uses presented challenges for both economic projections and retrofit measure construction.

  1. A very thin light sheet technique used to investigate meniscus shapes by laser induced fluorescence

    International Nuclear Information System (INIS)

    Khan, M.A.

    2003-01-01

    In this paper a light sheet technique is described to accurately (50 μm) measure meniscus profiles in film formation problems. The use of a slit to create the thin (0.1 mm) laser sheet makes the technique easy to implement, and allows tunable sheet thickness. The low light intensity obtained through the slit is compensated by the induced fluorescence of the tested fluid, which provides good picture contrast. After video recording through a microscope, the actual meniscus is recovered by image processing and proper calibration. The efficiency of the technique is demonstrated on a coating flow experiment. Due to its good accuracy and ease of use, this technique is expected to provide useful quantitative information about meniscus problems, in particular for the validation of computational fluid dynamics CFD solutions of coating flows. (author)

  2. Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains.

    Science.gov (United States)

    Buckley, Anthony M; Jukes, Caitlin; Candlish, Denise; Irvine, June J; Spencer, Janice; Fagan, Robert P; Roe, Andrew J; Christie, John M; Fairweather, Neil F; Douce, Gillian R

    2016-03-21

    The uses of fluorescent reporters derived from green fluorescent protein have proved invaluable for the visualisation of biological processes in bacteria grown under aerobic conditions. However, their requirement for oxygen has limited their application in obligate anaerobes such as Clostridium difficile. Fluorescent proteins derived from Light, Oxygen or Voltage sensing (LOV) domains have been shown to bridge this limitation, but their utility as translational fusions to monitor protein expression and localisation in a strict anaerobic bacterium has not been reported. Here we demonstrate the utility of phiLOV in three species of Clostridium and its application as a marker of real-time protein translation and dynamics through genetic fusion with the cell division protein, FtsZ. Time lapse microscopy of dividing cells suggests that Z ring assembly arises through the extension of the FtsZ arc starting from one point on the circumference. Furthermore, through incorporation of phiLOV into the flagella subunit, FliC, we show the potential of bacterial LOV-based fusion proteins to be successfully exported to the extracellular environment.

  3. Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration

    Science.gov (United States)

    Ando, Masatoshi; Ferreira-Zandoná, Andrea G.; Eckert, George J.; Zero, Domenick T.; Stookey, George K.

    2017-03-01

    This study aimed to evaluate the ability of quantitative light-induced fluorescence (QLF) to assess caries lesion activity using visual examination (VE) as the gold standard. Twenty-four visible white spot lesions on buccal surfaces were examined from 23 children, ages 9 to 14 years. At baseline, the surface was hydrated with water, and thereafter, it was dehydrated with continuous compressed air during image acquisition. QLF images were acquired at 0 (baseline), 5, and 15 s. QLF variables [QLFV: fluorescence loss (ΔF), lesion size (S), ΔQ: ΔF×S] was recorded. Changes-in-QLFV per second (ΔQLFV) were determined: ΔQLFV=(QLFVN-QLF/N), where N indicates dehydration time. One experienced dentist conducted VE independently using a dental unit's light, compressed air, and explorer. QLFV and ΔQLFV of the active group (n=11) were compared with those of the inactive group (n=13) using two-sample t-tests. As the surface was dehydrated, S and ΔQ values of the active group increased, whereas QLFV of the inactive group showed only a small change. ΔQLFV of the active group were larger than those of the inactive group; however, the difference did not reach statistical significance (p>0.11). Within the limitations of this study, QLF data indicated increments for lesions designated as active and minimal change for lesions defined as inactive.

  4. Polychromatic light-emitting diodes with a fluorescent nanosphere opal coating

    International Nuclear Information System (INIS)

    Hui, K N; Fu, W Y; Ng, W N; Leung, C H; Lai, P T; Wong, K K Y; Choi, H W

    2008-01-01

    A hexagonally close-packed array consisting of fluorescent nanospheres was coated onto short-wavelength GaN light-emitting diodes to demonstrate polychromatic white light emission. The spherical particles self-assemble into ordered three-dimensional opal structures, performing the role of color conversion to generate a polychromatic spectrum with smooth and uniform emission patterns. Different ratios of green and orange-red fluorescent nanospheres were mixed and coated onto high-extraction-efficiency micro-LEDs. Four devices with different shades of white emission were demonstrated. Device A, with a high content of orange-red nanospheres, offers the highest CRI value of 80, whereas device C with a well-balanced ratio of green and orange-red nanospheres exhibits color characteristics closest to ideal white with CIE coordinate at (0.34, 0.34). At 20 mA driving current, the luminous efficacy of the devices A, B, C, and D are 40.5 lm W -1 , 57.7 lm W -1 , 63.1 lm W -1 , and 67.2 lm W -1 respectively, while the correlated color temperatures (CCTs) of the corresponding devices are 3587, 4778, 5271, and 13 000 K

  5. Retrofitting of NPP Computer systems

    International Nuclear Information System (INIS)

    Pettersen, G.

    1994-01-01

    Retrofitting of nuclear power plant control rooms is a continuing process for most utilities. This involves introducing and/or extending computer-based solutions for surveillance and control as well as improving the human-computer interface. The paper describes typical requirements when retrofitting NPP process computer systems, and focuses on the activities of Institute for energieteknikk, OECD Halden Reactor project with respect to such retrofitting, using examples from actual delivery projects. In particular, a project carried out for Forsmarksverket in Sweden comprising upgrade of the operator system in the control rooms of units 1 and 2 is described. As many of the problems of retrofitting NPP process computer systems are similar to such work in other kinds of process industries, an example from a non-nuclear application area is also given

  6. Retrofitting for fossil fuel flexibility

    International Nuclear Information System (INIS)

    Newell, J.; Trueblood, R.C.; Lukas, R.W.; Worster, C.M.; Marx, P.D.

    1991-01-01

    Described in this paper are two fossil plant retrofits recently completed by the Public Service Company of New Hampshire that demonstrate the type of planning and execution required for a successful project under the current regulatory and budget constraints. Merrimack Units 1 and 2 are 120 MW and 338 MW nominal cyclone-fired coal units in Bow, New Hampshire. The retrofits recently completed at these plants have resulted in improved particulate emissions compliance, and the fuel flexibility to allow switching to lower sulphur coals to meet current and future SO 2 emission limits. Included in this discussion are the features of each project including the unique precipitator procurement approach for the Unit 1 Retrofit, and methods used to accomplish both retrofits within existing scheduled maintenance outages through careful planning and scheduling, effective use of pre-outage construction, 3-D CADD modeling, modular construction and early procurement. Operating experience while firing various coals in the cyclone fired boilers is also discussed

  7. Advancing Residential Retrofits in Atlanta

    Energy Technology Data Exchange (ETDEWEB)

    Jackson, Roderick K [ORNL; Kim, Eyu-Jin [Southface Energy Institute; Roberts, Sydney [Southface Energy Institute; Stephenson, Robert [Southface Energy Institute

    2012-07-01

    This report will summarize the home energy improvements performed in the Atlanta, GA area. In total, nine homes were retrofitted with eight of the homes having predicted source energy savings of approximately 30% or greater based on simulated energy consumption.

  8. Consumer preferences and willingness to pay for compact fluorescent lighting: Policy implications for energy efficiency promotion in Saint Lucia

    International Nuclear Information System (INIS)

    Reynolds, Travis; Kolodinsky, Jane; Murray, Byron

    2012-01-01

    This article examines consumer willingness to pay for energy-saving compact fluorescent light bulbs using the results of a stated preferences study conducted in the Caribbean island nation of Saint Lucia. Geographic location, low income status, and age are found to affect willingness-to-pay for compact fluorescent lighting, while higher income status and other demographic variables appear to have minimal or no significant impacts. Energy efficiency knowledge is associated with increased willingness-to-pay for energy-efficient bulbs and with increased use of compact fluorescent lighting. Contrary to theoretical expectations, past purchase of compact fluorescent bulbs is found to have no impact on self-reported willingness to pay. We hypothesize that this null result is due to the recent emergence of low-cost, low-quality compact fluorescent bulbs in the Saint Lucian lighting market, which may be negatively influencing consumers' preferences and expectations regarding energy-efficient lighting. Findings support the argument that government-sponsored education and subsidy programs will likely result in increased use of energy-saving technologies in Saint Lucia. But such behavioral changes may not be sustained in the long run unless low quality bulbs – the “lemons” of the compact fluorescent lighting market – can be clearly identified by consumers. - Highlights: ▶ We model how knowledge, attitudes, and past purchase affect CFL adoption. ▶ Saint Lucian consumers have some knowledge of and favorable attitudes toward CFLs. ▶ Energy efficiency knowledge increases stated willingness-to-pay (WTP) for CFLs. ▶ Past purchase does not increase WTP; low-quality ‘lemons’ may influence consumers. ▶ Policy can lower consumer risks in lighting markets where low quality bulbs exist.

  9. High-efficiency fluorescent organic light-emitting devices using sensitizing hosts with a small singlet-triplet exchange energy.

    Science.gov (United States)

    Zhang, Dongdong; Duan, Lian; Li, Chen; Li, Yilang; Li, Haoyuan; Zhang, Deqiang; Qiu, Yong

    2014-08-06

    Materials with small singlet-triplet splits (ΔEST s) are introduced as sensitizing hosts to excite fluorescent dopants, breaking the trade-off between small ΔEST and high radiative decay rates. A highly efficient orange-fluorescent organic light-emitting diode (OLED) is prepared, showing a maximum external quantum efficiency of 12.2%. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Advanced lighting guidelines: 1993. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Eley, C.; Tolen, T.M. [Eley Associates, San Francisco, CA (United States); Benya, J.R. [Luminae Souter Lighting Design, San Francisco, CA (United States); Rubinstein, F.; Verderber, R. [Lawrence Berkeley Lab., CA (United States)

    1993-12-31

    The 1993 Advanced Lighting Guidelines document consists of twelve guidelines that provide an overview of specific lighting technologies and design application techniques utilizing energy-efficient lighting practice. Lighting Design Practice assesses energy-efficient lighting strategies, discusses lighting issues, and explains how to obtain quality lighting design and consulting services. Luminaires and Lighting Systems surveys luminaire equipment designed to take advantage of advanced technology lamp products and includes performance tables that allow for accurate estimation of luminaire light output and power input. The additional ten guidelines -- Computer-Aided Lighting Design, Energy-Efficient Fluorescent Ballasts, Full-Size Fluorescent Lamps, Compact Fluorescent Lamps, Tungsten-Halogen Lamps, Metal Halide and HPS Lamps, Daylighting and Lumen Maintenance, Occupant Sensors, Time Scheduling Systems, and Retrofit Control Technologies -- each provide a product technology overview, discuss current products on the lighting equipment market, and provide application techniques. This document is intended for use by electric utility personnel involved in lighting programs, lighting designers, electrical engineers, architects, lighting manufacturers` representatives, and other lighting professionals.

  11. Light-emitting diode and laser fluorescence-based devices in detecting occlusal caries

    Science.gov (United States)

    Rodrigues, Jonas A.; Hug, Isabel; Neuhaus, Klaus W.; Lussi, Adrian

    2011-10-01

    The aim of this study was to assess the performance of two light-emitting diode (LED)- and two laser fluorescence-based devices in detecting occlusal caries in vitro. Ninety-seven permanent molars were assessed twice by two examiners using two LED- (Midwest Caries - MID and VistaProof - VP) and two laser fluorescence-based (DIAGNOdent 2095 - LF and DIAGNOdent pen 2190 - LFpen) devices. After measuring, the teeth were histologically prepared and classified according to lesion extension. At D1 the specificities were 0.76 (LF and LFpen), 0.94 (MID), and 0.70 (VP); the sensitivities were 0.70 (LF), 0.62 (LFpen), 0.31 (MID), and 0.75 (VP). At D3 threshold the specificities were 0.88 (LF), 0.87 (LFpen), 0.90 (MID), and 0.70 (VP); the sensitivities were 0.63 (LF and LFpen), 0.70 (MID), and 0.96 (VP). Spearman's rank correlations with histology were 0.56 (LF), 0.51 (LFpen), 0.55 (MID), and 0.58 (VP). Inter- and intraexaminer ICC values were high and varied from 0.83 to 0.90. Both LF devices seemed to be useful auxiliary tools to the conventional methods, presenting good reproducibility and better accuracy at D3 threshold. MID was not able to differentiate sound surfaces from enamel caries and VP still needs improvement on the cut-off limits for its use.

  12. Determination of RNA degradation by capillary electrophoresis with cyan light-emitted diode-induced fluorescence.

    Science.gov (United States)

    Yang, Tzu-Hsueh; Chang, Po-Ling

    2012-05-25

    RNA integrity plays an important role in RNA studies because poor RNA quality may have a great impact on downstream methodologies. This study proposes a cost-effective, rapid, and sensitive method for determining RNA integrity based on capillary electrophoresis that utilizes a cyan light-emitted diode-induced fluorescence as a separation tool. The capillary was initially coated with 0.1% Poly(vinylpyrrolidone) (M(ave) 1,300,000 Da) to reduce electroosmotic flow and avoid RNA adsorption. When the capillary was filled with 0.4% poly(ethylene) oxide (M(ave) 4,000,000) and a nucleic acid-specific fluorescent dye, SYTO 9, the baseline separation of the 18S and 28S ribosomal RNAs (rRNAs) in total RNA was accomplished within 15 min. The lowest detectable concentration for the 18S and 28S rRNAs was estimated to be 50 pg/μL. Some peaks longer than the 28S rRNA that migrated slowly were observed as long as the initial total RNA concentration was optimized. The temperature-induced degradation of the large RNA fragments (longer than the 28S rRNA) was faster than that of 18S rRNA and 28S rRNA. These large RNA fragments may serve as a promising marker for testing RNA integrity compared to the traditional method. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. Aggregation of Non Ionic Surfactant Igepal in Aqueous Solution: Fluorescence and Light Scattering Studies

    Directory of Open Access Journals (Sweden)

    Subhash Ch. Bhattacharya

    2003-10-01

    Full Text Available Abstract: The association of the polyoxyethylene nonyl phenol (Igepal in aqueous solution has been studied. The results of the fluorescence quenching of 1-Anthracene sulphonate and Safranine T in nonionic micellar solution of Igepal have been presented. The quenching process was exploited to estimate the aggregation number of surfactant monomer. In the Igepal series the micellar aggregation number systematically varied. From the dynamic light scattering studies in micellar solution the polydispersity of the medium and the diameter of the micelles have been determined. The diameters of the micelles have been increased with increase in the molar mass of surfactants. A reasonable estimation of the surface area of the head group in different micelles has been attempted.

  14. Gating circuit for single photon-counting fluorescence lifetime instruments using high repetition pulsed light sources

    International Nuclear Information System (INIS)

    Laws, W.R.; Potter, D.W.; Sutherland, J.C.

    1984-01-01

    We have constructed a circuit that permits conventional timing electronics to be used in single photon-counting fluorimeters with high repetition rate excitation sources (synchrotrons and mode-locked lasers). Most commercial time-to-amplitude and time-to-digital converters introduce errors when processing very short time intervals and when subjected to high-frequency signals. This circuit reduces the frequency of signals representing the pulsed light source (stops) to the rate of detected fluorescence events (starts). Precise timing between the start/stop pair is accomplished by using the second stop pulse after a start pulse. Important features of our design are that the circuit is insensitive to the simultaneous occurrence of start and stop signals and that the reduction in the stop frequency allows the start/stop time interval to be placed in linear regions of the response functions of commercial timing electronics

  15. Active Appearance Segmentation for Intensity Inhomogeneity in Light Sheet Fluorescence Microscopy

    DEFF Research Database (Denmark)

    Jensen, Casper Bo; Lyksborg, Mark; Hecksher-Sørensen, J.

    2016-01-01

    Active Appearance Models (AAM) are used for annotating or segmenting shapes in biomedical images. Performance relies heavily on the image data used to train the AAM. In this paper we improve the generalization properties of the model by making it robust to slowly varying spatial intensity...... inhomogeneities which are often seen in Light Sheet Fluorescence Microscopy (LSFM) images. This robustness is achieved by modelling the appearance of an image as a regularized Normalized Gradient Field (rNGF). We perform two experiments to challenge the model. First it is tested using a repeated leave......-one-out approach on images with minimal imperfections where the left out images are corrupted by a simulated bias field and segmented using the AAM. Secondly we test the model on LSFM images with common acquisition problems. In both experiments the proposed approach outperforms the often used AAM implementation...

  16. Detection and Analysis of Enamel Cracks by Quantitative Light-induced Fluorescence Technology.

    Science.gov (United States)

    Jun, Mi-Kyoung; Ku, Hye-Min; Kim, Euiseong; Kim, Hee-Eun; Kwon, Ho-Keun; Kim, Baek-Il

    2016-03-01

    The ability to accurately detect tooth cracks and quantify their depth would allow the prediction of crack progression and treatment success. The aim of this in vitro study was to determine the capabilities of quantitative light-induced fluorescence (QLF) technology in the detection of enamel cracks. Ninety-six extracted human teeth were selected for examining naturally existing or suspected cracked teeth surfaces using a photocuring unit. QLF performed with a digital camera (QLF-D) images were used to assess the ability to detect enamel cracks based on the maximum fluorescence loss value (ΔFmax, %), which was then analyzed using the QLF-D software. A histologic evaluation was then performed in which the samples were sectioned and observed with the aid of a polarized light microscope. The relationship between ΔFmax and the histology findings was assessed based on the Spearman rank correlation. The sensitivity and specificity were calculated to evaluate the validity of using QLF-D to analyze enamel inner-half cracks and cracks extending to the dentin-enamel junction. There was a strong correlation between the results of histologic evaluations of enamel cracks and the ΔFmax value, with a correlation coefficient of 0.84. The diagnostic accuracy of QLF-D had a sensitivity of 0.87 and a specificity of 0.98 for enamel inner-half cracks and a sensitivity of 0.90 and a specificity of 1.0 for cracks extending to the dentin-enamel junction. These results indicate that QLF technology would be a useful clinical tool for diagnosing enamel cracks, especially given that this is a nondestructive method. Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

  17. Is the light-emitting diode a better light source than fluorescent tube for phototherapy of neonatal jaundice in preterm infants?

    OpenAIRE

    Mohammadizadeh, Majid; Eliadarani, Fereshteh Kadkhodaei; Badiei, Zohreh

    2012-01-01

    Background: Light-emitting diodes (LEDs) are light sources recently used for phototherapy in neonatal jaundice. We compared the efficacy and safety of LEDs with fluorescent phototherapy in the treatment of indirect hyperbilirubinemia. Materials and Methods: This controlled trial was conducted on preterm infants hospitalized in neonatal intensive care unit of Shahid Beheshti Hospital in Isfahan (Iran) who needed conventional phototherapy for uncomplicated indirect hyperbilirubinemia. Neon...

  18. Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D).

    Science.gov (United States)

    Lee, Eun-Song; Kang, Si-Mook; Ko, Hae-Youn; Kwon, Ho-Keun; Kim, Baek-Il

    2013-12-01

    This study evaluated whether Quantitative Light-induced Fluorescence-Digital (QLF-D) can detect the levels of cariogenicity of dental microcosm biofilms by assessing the red fluorescence intensity. Dental microcosm biofilms were initiated from human saliva on bovine enamel discs. Biofilms with various levels of cariogenicity were then grown in artificial saliva supplemented with sucrose at different concentrations (0.05%, 0.1%, 0.2%, and 0.5%) in 24-well microplates. After 10 days, fluorescence images of the biofilms were captured by the QLF-D to analyse the red fluorescence intensity, which was quantified as the red/green ratio (R/G value). The supernatant pH was also measured, as well as the total and aciduric bacteria counts of the collected biofilms. Mineral loss in enamel was also evaluated by calculating the percentage of surface microhardness changes (%SHC). The R/G values of the biofilms differed significantly with the sucrose concentration (pdental microcosm biofilms in vitro, which indicates that this device can be used to detect the levels of cariogenicity of a dental biofilm. The QLF-D is able to assess the cariogenic levels of dental plaque based on the intensity of red fluorescence. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Retrofitting a 1960s Split-Level, Cold-Climate Home

    Energy Technology Data Exchange (ETDEWEB)

    Puttagunta, Srikanth [Consortium for Advanced Residential Buildings, Norwalk, CT (United States)

    2015-07-01

    National programs such as Home Performance with ENERGY STAR® and numerous other utility air sealing programs have brought awareness to homeowners of the benefits of energy efficiency retrofits. Yet, these programs tend to focus on the low-hanging fruit: air-sealing the thermal envelope and ductwork where accessible, switch to efficient lighting, and low-flow fixtures. At the other end of the spectrum, deep-energy retrofit programs are also being encouraged by various utilities across the country. While deep energy retrofits typically seek 50% energy savings, they are often quite costly and most applicable to gut-rehab projects. A significant potential for lowering energy usage in existing homes lies between the low hanging fruit and deep energy retrofit approaches - retrofits that save approximately 30% in energy over the existing conditions. A key is to be non-intrusive with the efficiency measures so the retrofit projects can be accomplished in occupied homes. This cold climate retrofit project involved the design and optimization of a home in Connecticut that sought to improve energy savings by at least 30% (excluding solar PV) over the existing home's performance. This report documents the successful implementation of a cost-effective solution package that achieved performance greater than 30% over the pre-retrofit - what worked, what did not, and what improvements could be made.

  20. Seismic retrofit benefit considering statewide transportation assessment.

    Science.gov (United States)

    2015-06-01

    The purpose of this study was to identify and demonstrate a methodology to prioritize bridges for : retrofit in the State of Oregon. Given the limited resources available, retrofitting all vulnerable bridges in the foreseeable : future would not be i...

  1. Green Retrofitting Skyscrapers: A Review

    Directory of Open Access Journals (Sweden)

    Kheir Al-Kodmany

    2014-09-01

    Full Text Available This paper investigates innovative trends, practices and goals of tall building retrofits while illustrating green design techniques and implementation strategies. The existing building stock is substantially large and represents one of the biggest opportunities to reduce energy waste and curb air pollution and global warming. In terms of tall buildings, many will benefit from retrofits. There are long lists of inefficient all-glass curtain walls, initially promoted by the modernist movement, that are due to retrofit. The all-glass curtain wall buildings rely on artificial ventilation, cooling and heating, and suffer from poor insulation, which collectively make them energy hogs. Recent practices indicate that green retrofit has helped older buildings to increase energy efficiency, optimize building performance, increase tenants’ satisfaction and boost economic return while reducing greenhouse gas emission. As such, renovating older buildings could be “greener” than destroying them and rebuilding new ones. While some demolition and replacement may remain a necessity to meet contemporary needs, there are significant opportunities to reduce carbon emission and improve existing buildings’ performance by retrofitting them rather than constructing new ones. Practical insight indicates that the confluence of economic and environmental goals is increasingly at the heart of sustainable planning and design.

  2. Wide-field imaging through scattering media by scattered light fluorescence microscopy

    Science.gov (United States)

    Zhou, Yulan; Li, Xun

    2017-08-01

    To obtain images through scattering media, scattered light fluorescence (SLF) microscopy that utilizes the optical memory effect has been developed. However, the small field of view (FOV) of SLF microscopy limits its application. In this paper, we have introduced a re-modulation method to achieve wide-field imaging through scattering media by SLF microscopy. In the re-modulation method, to raster scan the focus across the object plane, the incident wavefront is re-modulated via a spatial light modulator (SLM) in the updated phase compensation calculated using the optimized iterative algorithm. Compared with the conventional optical memory effect method, the re-modulation method can greatly increase the FOV of a SLF microscope. With the phase compensation theoretically calculated, the process of updating the phase compensation of a high speed SLM is fast. The re-modulation method does not increase the imaging time. The re-modulation method is, therefore, expected to make SLF microscopy have much wider applications in biology, medicine and physiology.

  3. Strategy Guideline. Mitigation of Retrofit Risk Factors

    Energy Technology Data Exchange (ETDEWEB)

    Berman, M. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Smith, P. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Porse, E. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States)

    2012-12-01

    The Alliance for Residential Building Innovation (ARBI) Building America team is currently developing strategies designed to promote and achieve increased energy savings and promote upgrades in the residential retrofit sector. These strategies are targeted to retrofit program managers, retrofit contractors, policy makers, academic researchers, and non-governmental organizations. This report focuses on four key areas to promote home energy upgrades: fostering accurate energy savings projections; understanding consumer perceptions for energy savings; measuring energy savings, and ensuring quality control for retrofit installations.

  4. Strategy Guideline: Mitigation of Retrofit Risk Factors

    Energy Technology Data Exchange (ETDEWEB)

    Berman, M.; Smith, P.; Porse, E.

    2012-12-01

    The Alliance for Residential Building Innovation (ARBI) is currently developing strategies designed to promote and achieve increased energy savings and promote upgrades in the residential retrofit sector. These strategies are targeted to retrofit program managers, retrofit contractors, policy makers, academic researchers, and non-governmental organizations. This report focuses on four key areas to promote home energy upgrades: fostering accurate energy savings projections; understanding consumer perceptions for energy savings; measuring energy savings, and ensuring quality control for retrofit installations.

  5. Light stress effect and by nitrogen deficiency in plants of Petiveria alliacea measured with two-chlorophyll-fluorescence technique

    Science.gov (United States)

    Zuluaga, H.; Oviedo, A.; Solarte, Efrain; Pena, E. J.

    2004-10-01

    The chlorophyll fluorescence was studied in Petiveria alliacea plants exposed to different nitrogen concentrations and light radiation, the response was measured by two different forms; (1) measuring the photosynthetic efficiency with a pulse amplitude modulated fluorometro (PAM) emitted by a 650 nm diode and (2) measuring the fluorescence spectra caused by high power 452 nm diode with a SD2000 spectrometer. It was found out that the photosynthetic efficiency decreased in the plants exposed to high radiance and low nitrogen. Two chlorophyll fluorescence peaks were observed on 684 nm and 739 nm, the intensities in this wavelengths are inversely related with the light radiance. The correlation between the FIR and photosynthetic efficiency was very strong (r2 = -0.809, p Alliacea plants.

  6. Dynamic Response of Plant Chlorophyll Fluorescence to Light, Water and Nutrient Availability

    Science.gov (United States)

    Cendrero Mateo, M. D. P.; Moran, S. M.; Porcar-Castell, A.; Carmo-Silva, A. E.; Papuga, S. A.; Matveeva, M.; Wieneke, S.; Rascher, U.

    2014-12-01

    Photosynthesis is the most important exchange process of CO2 between the atmosphere and the land-surface. Spatial and temporal patterns of photosynthesis depend on dynamic plant-specific adaptation strategies to highly variable environmental conditions e.g. light, water, and nutrient availability. Chlorophyll fluorescence (ChF) has been proposed as a direct indicator of photosynthesis, and several studies have demonstrated its relationship with vegetation functioning at leaf and canopy level. In this study, two overarching questions about ChF were addressed: Q1) How water, nutrient and ambient light conditions determine the relationships between photosynthesis and ChF? Which is the optimum irradiance level for detecting water and nutrient deficit conditions with ChF?; Q2) What is the seasonal relationship between photosynthesis and ChF when nitrogen is the limiting factor? The results of this study indicated that when the differences between treatments (water or nitrogen) drive the relationship between photosynthesis and ChF, ChF has a direct relationship with photosynthesis. This study demonstrates that the light level at which plants were grown was optimum for detecting water and nutrient deficit with ChF. Further, the seasonal relation between photosynthesis and ChF with nitrogen stress was not a simple linear function due to the complex physiological relation between photosynthesis and ChF. Our study showed that at times in the season when nitrogen was sufficient and photosynthesis was highest, ChF decreased because these two processes compete for available energy. The results from this study demonstrated that ChF is a reliable indicator of plant stress and has great potential as a tool for better understand where, when, and how CO2 is exchanged between the land and atmosphere.

  7. Elemental mercury emission in the indoor environment due to broken compact fluorescent light (CFL) bulbs--paper

    Science.gov (United States)

    Compact fluorescent light (CFL) bulbs contain a few milligrams (mg) of elemental mercury. When a CFL breaks, some of the mercury is immediately released as elemental mercury vapor and the remainder is deposited on indoor surfaces with the bulb debris. In a controlled study design...

  8. Spectral trends in the fluorescence of single bacterial light-harvesting complexes: Experiments and modified redfield simulations

    NARCIS (Netherlands)

    Rutkauskas, D.; Novoderezhkin, V.; Gall, A.; Olsen, J.; Cogdell, R.J.; Hunter, C.N.; van Grondelle, R.

    2006-01-01

    In this work we present and discuss the single-molecule fluorescence spectra of a variety of species of light-harvesting complexes: LH2 of Rhodopseudomonas acidophila, Rhodobacter sphaeroides, and Rhodospirillum molischianum and LH1 of Rhodobacter sphaeroides. The emission spectrum of these

  9. Do daily and seasonal trends in leaf solar induced fluorescence reflect changes in photosynthesis, growth or light exposure

    Czech Academy of Sciences Publication Activity Database

    Wyber, R.; Malenovský, Zbyněk; Ashcroft, M. B.; Osmond, C. B.; Robinson, S. A.

    2017-01-01

    Roč. 9, č. 6 (2017), č. článku 604. ISSN 2072-4292 Institutional support: RVO:67179843 Keywords : Light induced fluorescence transient * Photosynthetic active radiation * Photosynthetic yield * Pulse amplitude modulation * Remote sensing of vegetation Subject RIV: EH - Ecology, Behaviour OBOR OECD: Environmental sciences (social aspects to be 5.7) Impact factor: 3.244, year: 2016

  10. Energy Retrofit for Aging K-12 Schools.

    Science.gov (United States)

    3D/International, Houston, TX.

    Successfully retrofitting aging K-12 schools using energy conservation measures (ECM) that can improve the physical plant and reduce energy consumption are explored. Topics explore how certain ECM measures can benefit educational facilities, why retrofitting begun sooner rather than later is important, how to finance the retrofit program, and the…

  11. EMCS Retrofit Analysis - Interim Report

    Energy Technology Data Exchange (ETDEWEB)

    Diamond, R.C.; Salsbury, T.I.; Bell, G.C.; Huang, Y.J.; Sezgen, A.O.; Mazzucchi, R.; Romberger, J.

    1999-03-01

    This report presents the interim results of analyses carried out in the Phillip Burton Federal Building in San Francisco from 1996 to 1998. The building is the site of a major demonstration of the BACnet communication protocol. The energy management and control systems (EMCS) in the building were retrofitted with BACnet compatible controllers in order to integrate certain existing systems on one common network. In this respect, the project has been a success. Interoperability of control equipment from different manufacturers has been demonstrated in a real world environment. Besides demonstrating interoperability, the retrofits carried out in the building were also intended to enhance control strategies and capabilities, and to produce energy savings. This report presents analyses of the energy usage of HVAC systems in the building, control performance, and the reaction of the building operators. The report does not present an evaluation of the performance capabilities of the BACnet protocol. A monitoring system was installed in the building that parallels many of the EMCS sensors and data were archived over a three-year period. The authors defined pre-retrofit and post-retrofit periods and analyzed the corresponding data to establish the changes in building performance resulting from the retrofit activities. The authors also used whole-building energy simulation (DOE-2) as a tool for evaluating the effect of the retrofit changes. The results of the simulation were compared with the monitored data. Changes in operator behavior were assessed qualitatively with questionnaires. The report summarizes the findings of the analyses and makes several recommendations as to how to achieve better performance. They maintain that the full potential of the EMCS and associated systems is not being realized. The reasons for this are discussed along with possible ways of addressing this problem. They also describe a number of new technologies that could benefit systems of the type

  12. Is the light-emitting diode a better light source than fluorescent tube for phototherapy of neonatal jaundice in preterm infants?

    Science.gov (United States)

    Mohammadizadeh, Majid; Eliadarani, Fereshteh Kadkhodaei; Badiei, Zohreh

    2012-01-01

    Light-emitting diodes (LEDs) are light sources recently used for phototherapy in neonatal jaundice. We compared the efficacy and safety of LEDs with fluorescent phototherapy in the treatment of indirect hyperbilirubinemia. This controlled trial was conducted on preterm infants hospitalized in neonatal intensive care unit of Shahid Beheshti Hospital in Isfahan (Iran) who needed conventional phototherapy for uncomplicated indirect hyperbilirubinemia. Neonates received phototherapy through devices with LEDs or special blue fluorescent tubes. Primary outcomes included the rate of fall of total serum bilirubin (TSB, mg/dL/hour) and duration of phototherapy (hours). Secondary outcomes were treatment failure and side effects. A total of 64 infants with gestational age of 33.5 ± 1.2 weeks, chronological age of 73.0 [SE = 7.3] hours, and weight of 1757.5 ± 147.6 gram were enrolled. The rates of fall of TSB were 0.20 [SE = 0.03] and 0.12 [SE = 0.01] mg/dL/hour in the LED and fluorescent groups, respectively (P = 0.472). Treatment duration was 37.5 ± 26.8 and 45.3 ± 32.1 hours in the LED and fluorescent groups, respectively (P = 0.292). There was no treatment failure in the two groups. Mild hyperthermia was occurred in 3.1% and 28.1% of infants in the LED and fluorescent groups, respectively (P = 0.006). LED light source is as effective as fluorescent tubes for phototherapy of preterm infants with indirect hyperbilirubinemia. Considering less frequent side effects, less energy consumption, longer life span, and lower costs, LED phototherapy seems to be a better option than current conventional phototherapy.

  13. Is the light-emitting diode a better light source than fluorescent tube for phototherapy of neonatal jaundice in preterm infants?

    Directory of Open Access Journals (Sweden)

    Majid Mohammadizadeh

    2012-01-01

    Full Text Available Background: Light-emitting diodes (LEDs are light sources recently used for phototherapy in neonatal jaundice. We compared the efficacy and safety of LEDs with fluorescent phototherapy in the treatment of indirect hyperbilirubinemia. Materials and Methods: This controlled trial was conducted on preterm infants hospitalized in neonatal intensive care unit of Shahid Beheshti Hospital in Isfahan (Iran who needed conventional phototherapy for uncomplicated indirect hyperbilirubinemia. Neonates received phototherapy through devices with LEDs or special blue fluorescent tubes. Primary outcomes included the rate of fall of total serum bilirubin (TSB, mg/dL/hour and duration of phototherapy (hours. Secondary outcomes were treatment failure and side effects. Results: A total of 64 infants with gestational age of 33.5 ± 1.2 weeks, chronological age of 73.0 [SE = 7.3] hours, and weight of 1757.5 ± 147.6 gram were enrolled. The rates of fall of TSB were 0.20 [SE = 0.03] and 0.12 [SE = 0.01] mg/dL/hour in the LED and fluorescent groups, respectively (P = 0.472. Treatment duration was 37.5 ± 26.8 and 45.3 ± 32.1 hours in the LED and fluorescent groups, respectively (P = 0.292. There was no treatment failure in the two groups. Mild hyperthermia was occurred in 3.1% and 28.1% of infants in the LED and fluorescent groups, respectively (P = 0.006. Conclusions: LED light source is as effective as fluorescent tubes for phototherapy of preterm infants with indirect hyperbilirubinemia. Considering less frequent side effects, less energy consumption, longer life span, and lower costs, LED phototherapy seems to be a better option than current conventional phototherapy.

  14. Retrofitting of existing housing stock

    DEFF Research Database (Denmark)

    Unterrainer, Walter

    2015-01-01

    The paper is a case study in retrofitting an urban quarter with social housing of the 1950´s and 1960´s - a model project in Amberg/Germany. It documents and discusses applied ´acupuncture´ and innovative strategies on very different scales as well as on different layers, in order to meet the goal...... of a sustainable retrofitting project in a holistic sense: This included cultural, social, environmental and economic sustainability. The first phase of the project is built and the process included a high level of user participation. The project is an example of integral design from competition level...

  15. The growth and flowering of Hyacinthus orientalis L. Forced in pots under fluorescent light of different colours

    Directory of Open Access Journals (Sweden)

    Małgorzata Śmigielska

    2014-09-01

    Full Text Available Three hyacinth cultivars were forced under fluorescent lamps which emitted white, blue, green, yellow and red light. The plants started flowering in the first decade of February. The forcing period for two cultivars, ‘Anna Marie’ and ‘Blue Star’, was shortest under lamps emitting red light. The cultivar ‘White Pearl’ flowered equally early under lamps emitting red, white and blue light. The impact of light colour (wavelength on the leaf greenness index (SPAD was demonstrated. The photosynthetic activity of leaves was dependent on the cultivar. It was related both to the net rate of photosynthesis and the photosynthetic efficiency. Specific leaf area (SLA also depended on the cultivar. The level of SLA was related to the rate of photosynthesis and its efficiency. SLA was highest in all cultivars under green and yellow colour light. The chlorophyll content in the fresh and dry weight of leaves was highest under yellow light lamps.

  16. Deep Energy Retrofits - Eleven California Case Studies

    Energy Technology Data Exchange (ETDEWEB)

    Less, Brennan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Fisher, Jeremy [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Walker, Iain [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2012-10-01

    This research documents and demonstrates viable approaches using existing materials, tools and technologies in owner-conducted deep energy retrofits (DERs). These retrofits are meant to reduce energy use by 70% or more, and include extensive upgrades to the building enclosure, heating, cooling and hot water equipment, and often incorporate appliance and lighting upgrades as well as the addition of renewable energy. In this report, 11 Northern California (IECC climate zone 3) DER case studies are described and analyzed in detail, including building diagnostic tests and end-use energy monitoring results. All projects recognized the need to improve the home and its systems approximately to current building code-levels, and then pursued deeper energy reductions through either enhanced technology/ building enclosure measures, or through occupant conservation efforts, both of which achieved impressive energy performance and reductions. The beyond-code incremental DER costs averaged $25,910 for the six homes where cost data were available. DERs were affordable when these incremental costs were financed as part of a remodel, averaging a $30 per month increase in the net-cost of home ownership.

  17. Fluorescence lifetime analyses reveal how the high light-responsive protein LHCSR3 transforms PSII light-harvesting complexes into an energy-dissipative state.

    Science.gov (United States)

    Kim, Eunchul; Akimoto, Seiji; Tokutsu, Ryutaro; Yokono, Makio; Minagawa, Jun

    2017-11-17

    In green algae, light-harvesting complex stress-related 3 (LHCSR3) is responsible for the pH-dependent dissipation of absorbed light energy, a function vital for survival under high-light conditions. LHCSR3 binds the photosystem II and light-harvesting complex II (PSII-LHCII) supercomplex and transforms it into an energy-dissipative form under acidic conditions, but the molecular mechanism remains unclear. Here we show that in the green alga Chlamydomonas reinhardtii , LHCSR3 modulates the excitation energy flow and dissipates the excitation energy within the light-harvesting complexes of the PSII supercomplex. Using fluorescence decay-associated spectra analysis, we found that, when the PSII supercomplex is associated with LHCSR3 under high-light conditions, excitation energy transfer from light-harvesting complexes to chlorophyll-binding protein CP43 is selectively inhibited compared with that to CP47, preventing excess excitation energy from overloading the reaction center. By analyzing femtosecond up-conversion fluorescence kinetics, we further found that pH- and LHCSR3-dependent quenching of the PSII-LHCII-LHCSR3 supercomplex is accompanied by a fluorescence emission centered at 684 nm, with a decay time constant of 18.6 ps, which is equivalent to the rise time constant of the lutein radical cation generated within a chlorophyll-lutein heterodimer. These results suggest a mechanism in which LHCSR3 transforms the PSII supercomplex into an energy-dissipative state and provide critical insight into the molecular events and characteristics in LHCSR3-dependent energy quenching. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  18. High efficiency fluorescent tubes. An efficient solution for the economical lighting of industrial and tertiary buildings; Les tubes fluorescents haut rendement. Une solution performante pour l'eclairage economique des locaux indutriels et tertiaires

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    Fluorescent lamps are low pressure mercury vapor lamps (discharge lamps). The high efficiency fluorescent tubes have a greater lighting efficiency thanks to the integration of tri-chromatic powders emitting in the three fundamental colors (red, green, blue). Their useful lifetime is exceptionally long (90% of their initial flux after 12000 hours of use), and they require a lower maintenance with respect to the traditional fluorescent tubes. This document presents the characteristics and advantages of high efficiency fluorescent tubes for professional use and their cost and performance with respect to standard fluorescent tubes. (J.S.)

  19. Multicolor fluorescent light-emitting diodes based on cesium lead halide perovskite quantum dots

    International Nuclear Information System (INIS)

    Wang, Peng; Bai, Xue; Sun, Chun; Zhang, Xiaoyu; Zhang, Yu; Zhang, Tieqiang

    2016-01-01

    High quantum yield, narrow full width at half-maximum and tunable emission color of perovskite quantum dots (QDs) make this kind of material good prospects for light-emitting diodes (LEDs). However, the relatively poor stability under high temperature and air condition limits the device performance. To overcome this issue, the liquid-type packaging structure in combination with blue LED chip was employed to fabricate the fluorescent perovskite quantum dot-based LEDs. A variety of monochromatic LEDs with green, yellow, reddish-orange, and red emission were fabricated by utilizing the inorganic cesium lead halide perovskite quantum dots as the color-conversion layer, which exhibited the narrow full width at half-maximum (<35 nm), the relatively high luminous efficiency (reaching 75.5 lm/W), and the relatively high external quantum efficiency (14.6%), making it the best-performing perovskite LEDs so far. Compared to the solid state LED device, the liquid-type LED devices exhibited excellent color stability against the various working currents. Furthermore, we demonstrated the potential prospects of all-inorganic perovskite QDs for the liquid-type warm white LEDs.

  20. Quantification of Canine Dental Plaque Using Quantitative Light-Induced Fluorescence.

    Science.gov (United States)

    Wallis, Corrin; Gill, Yadvinder; Colyer, Alison; Davis, Ian; Allsopp, Judi; Komarov, Gleb; Higham, Susan; Harris, Stephen

    2016-03-01

    The aim of this work was to evaluate Quantitative Light-induced Fluorescence (QLF) as an alternative to the established Logan and Boyce method for determining plaque coverage of dogs' teeth. In a series of studies in conscious and anesthetized dogs, QLF showed good intra-photographer repeatability (coefficient of variation [CV] of 7.5% for undisclosed teeth) and inter-photographer reproducibility (CV of 3.2% for undisclosed teeth and 8.5% for disclosed teeth). The QLF software accurately identifies areas of plaque as demonstrated by comparison to the variability of 5 human scorers, manually marking plaque on QLF-acquired images (P = 0.1). There was good agreement with the modified Logan and Boyce method in the percentage reduction in plaque accumulation measured when dogs were fed an oral care chew versus no chew. To see a 15% difference in plaque accumulation, which is considered sufficient by the Veterinary Oral Health Council to differentiate between 2 treatments, a retrospective power analysis (90%) of the data established that only 7 dogs would be required, compared to 19 dogs for the modified Logan and Boyce method. QLF is a reliable method for measuring dental plaque in dogs with the added advantage that it is not subjective and requires fewer animals.

  1. Evaluation of Degradation in Nanofilled Adhesive Resins Using Quantitative Light-Induced Fluorescence

    Directory of Open Access Journals (Sweden)

    Tae-Young Park

    2014-01-01

    Full Text Available The aim of this study was to evaluate degradation in commercial dental nanofilled adhesive resins using quantitative light-induced fluorescence (QLF. Three adhesives were selected: D/E resin (DR, Single Bond Plus (SB, and G-Bond (GB. The adhesives were mixed with porphyrin for the QLF analysis. Specimens were prepared by dispensing blended adhesives into a flexible mold and polymerizing. Then, the QLF analysis of the specimens was done and the porphyrin values (Simple Plaque Score and ΔR were measured. After thermocycling of the specimens (5000 cycles, 5 to 55°C for the degradation, the specimens were assayed by QLF again. The porphyrin values were analyzed using paired t-test at a 95% confidence level. A significant reduction in SPS was observed in all groups after thermocycling. The ΔR significantly decreased after thermocycling except area ΔR30 of SB group. Overall, porphyrin values decreased after thermocycling which indicates that the degradation of the adhesive resins may be measured by the change of porphyrin value. The QLF method could be used to evaluate the degradation of adhesive resin.

  2. Irradiance Decay in Fluorescent and Light-emitting Diode-based Phototherapy Devices: A Pilot Study.

    Science.gov (United States)

    Olusanya, Bolajoko O; Osibanjo, Folashade B; Emokpae, Abieyuwa A; Slusher, Tina M

    2016-10-01

    We set out to determine the rate of decline of irradiance for fluorescent tube (FT) and light-emitting diode (LED) phototherapy devices in resource-limited settings where routine irradiance monitoring is uncommon. Irradiance levels (μW/cm(2)/nm) were measured weekly using BiliBlanket(®) II Meter on three FT-based and two LED-based phototherapy devices over a 19 week period. The two LED devices showed stable irradiance levels and did not require any lamp changes. The three FT-based devices showed rapid decline in irradiance, and all required three complete lamp exchanges approximately every 5-6 weeks. FT-based devices are associated with more rapid decline in irradiance to sub-therapeutic levels and require more frequent lamp changes than LED devices. Clinicians should be alert to the maintenance requirements of the phototherapy devices available in their settings to ensure efficacy of treatment. © The Author [2016]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  3. Sunnyvale Marine Climate Deep Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    German, A.; Siddiqui, A.; Dakin, B.

    2014-11-01

    The Alliance for Residential Building Innovation (ARBI) and Allen Gilliland of One Sky Homes collaborated on a marine climate retrofit project designed to meet both Passive House (PH) and Building America (BA) program standards. The scope included sealing, installing wall, roof and floor insulation (previously lacking), replacing windows, upgrading the heating and cooling system, and installing.

  4. Sunnyvale Marine Climate Deep Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    German, A. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Siddiqui, A. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Dakin, B. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States)

    2014-11-01

    The Alliance for Residential Building Innovation (ARBI) and Allen Gilliland of One Sky Homes collaborated on a marine climate retrofit project designed to meet both Passive House (PH) and Building America program standards. The scope included sealing, installing wall, roof and floor insulation (previously lacking), replacing windows, upgrading the heating and cooling system, and installing mechanical ventilation.

  5. Retrofitting of existing housing stock

    DEFF Research Database (Denmark)

    Unterrainer, Walter

    2015-01-01

    The paper is a case study in retrofitting an urban quarter with social housing of the 1950´s and 1960´s - a model project in Amberg/Germany. It documents and discusses applied ´acupuncture´ and innovative strategies on very different scales as well as on different layers, in order to meet the goa...

  6. Comparative Study of Lettuce and Radish Grown Under Red and Blue Light-Emitting Diodes (LEDs) and White Fluorescent Lamps

    Science.gov (United States)

    Mickens, Matthew A.

    2012-01-01

    Growing vegetable crops in space will be an essential part of sustaining astronauts during long-term missions. To drive photosynthesis, red and blue light-emitting diodes (LEDs) have attracted attention because of their efficiency, longevity, small size, and safety. In efforts to optimize crop production, there have also been recent interests in analyzing the subtle effects of green light on plant growth, and to determine if it serves as a source of growth enhancement or suppression. A comparative study was performed on two short cycle crops of lettuce (Outredgeous) and radish (Cherry Bomb) grown under two light treatments. The first treatment being red and blue LEDs, and the second treatment consisting of white fluorescent lamps which contain a portion of green light. In addition to comparing biomass production, physiological characterizations were conducted on how the light treatments influence morphology, water use, chlorophyll content, and the production of A TP within plant tissues.

  7. Modification of energy-transfer processes in the cyanobacterium, Arthrospira platensis, to adapt to light conditions, probed by time-resolved fluorescence spectroscopy.

    Science.gov (United States)

    Akimoto, Seiji; Yokono, Makio; Aikawa, Shimpei; Kondo, Akihiko

    2013-11-01

    In cyanobacteria, the interactions among pigment-protein complexes are modified in response to changes in light conditions. In the present study, we analyzed excitation energy transfer from the phycobilisome and photosystem II to photosystem I in the cyanobacterium Arthrospira (Spirulina) platensis. The cells were grown under lights with different spectral profiles and under different light intensities, and the energy-transfer characteristics were evaluated using steady-state absorption, steady-state fluorescence, and picosecond time-resolved fluorescence spectroscopy techniques. The fluorescence rise and decay curves were analyzed by global analysis to obtain fluorescence decay-associated spectra. The direct energy transfer from the phycobilisome to photosystem I and energy transfer from photosystem II to photosystem I were modified depending on the light quality, light quantity, and cultivation period. However, the total amount of energy transferred to photosystem I remained constant under the different growth conditions. We discuss the differences in energy-transfer processes under different cultivation and light conditions.

  8. Retrofitting a 1960s Split-Level, Cold-Climate Home

    Energy Technology Data Exchange (ETDEWEB)

    Puttagunta, Srikanth [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2015-07-13

    National programs such as Home Performance with ENERGY STAR® and numerous other utility air-sealing programs have made homeowners aware of the benefits of energy-efficiency retrofits. Yet these programs tend to focus only on the low-hanging fruit: they recommend air sealing the thermal envelope and ductwork where accessible, switching to efficient lighting and low-flow fixtures, and improving the efficiency of mechanical systems (though insufficient funds or lack of knowledge to implement these improvements commonly prevent the implementation of these higher cost upgrades). At the other end of the spectrum, various utilities across the country are encouraging deep energy retrofit programs. Although deep energy retrofits typically seek 50% energy savings, they are often quite costly and are most applicable to gut-rehab projects. A significant potential for lowering energy use in existing homes lies between the lowhanging fruit and deep energy retrofit approaches—retrofits that save approximately 30% in energy compared to the pre-retrofit conditions. The energy-efficiency measures need to be nonintrusive so the retrofit projects can be accomplished in occupied homes.

  9. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

    OpenAIRE

    Hofmann, M.; Eggeling, C.; Jakobs, S.; Hell, S.

    2005-01-01

    Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated depletion of the marker's fluorescent state by stimulated emission, but this approach requires picosecond laser pulses of GW/cm2 intensity. Here, we demonstrate the surpassing of the diffraction barrier in fluorescence microscopy with illumination intensities that are eight orders of magn...

  10. SU-E-I-21: Effect of Different Fluorescent Lights with Various Colors of a Reading Room on Chromaticity of LCD.

    Science.gov (United States)

    Yano, Y; Akamine, H; Awamoto, S; Nakamura, Y; Morishita, J

    2012-06-01

    To examine variation of chromaticity of LCD in different types of fluorescent lights in a reading room. A color LCD (RX320, antiglare type, 450 cd/m 2 , three-megapixel, Eizo Nanao), and a monochrome LCD (G31-S, anti glare type, 450 cd/m 2 , three-megapixel, Eizo Nanao) were used in this study. The chromaticity in grayscale images with eighteen luminance levels were measured under five types of fluorescent lights with different color spectrums (Daylight: 6,700 K, Natural white: 5,000 K, White: 4,200 K, Warm white: 3,500 K, Light bulb: 3,000 K) by using a colorimeter (CS-200: KONICA MINOLTA). The chromaticity of LCDs was measured at various ambient lighting conditions (a dark room, 36, and 300 lux) and different types of fluorescent lights. The chromaticity of LCDs measured under ambient lights was changed from that measured in a dark room. The chromaticity of LCDs varied with different types of fluorescent lights. As illuminance of the room increased, variations in chromaticity at relatively lower luminance levels increased. The direction of changes in chromaticity shifted to the color for each fluorescent light. Fluorescent lights having different color spectra affect the chromaticity of LCDs. © 2012 American Association of Physicists in Medicine.

  11. Optimizing Whole House Deep Energy Retrofit Packages: A Case Study of Existing Chicago-Area Homes

    Directory of Open Access Journals (Sweden)

    Honnie Aguilar Leinartas

    2015-05-01

    Full Text Available Improving the energy efficiency of the residential building stock plays a key role in mitigating global climate change. New guidelines are targeting widespread application of deep energy retrofits to existing homes that reduce their annual energy use by 50%, but questions remain as to how to identify and prioritize the most cost-effective retrofit measures. This work demonstrates the utility of whole building energy simulation and optimization software to construct a “tool-box” of prescriptive deep energy retrofits that can be applied to large portions of the existing housing stock. We consider 10 generally representative typology groups of existing single-family detached homes built prior to 1978 in the Chicago area for identifying cost-optimal deep energy retrofit packages. Simulations were conducted in BEopt and EnergyPlus operating on a cloud-computing platform to first identify cost-optimal enclosure retrofits and then identify cost-optimal upgrades to heating, ventilation, and air-conditioning (HVAC systems. Results reveal that prescriptive retrofit packages achieving at least 50% site energy savings can be defined for most homes through a combination of envelope retrofits, lighting upgrades, and upgrades to existing HVAC system efficiency or conversion to mini-split heat pumps. The average upfront cost of retrofits is estimated to be ~$14,400, resulting in average annual site energy savings of ~54% and an average simple payback period of ~25 years. Widespread application of these prescriptive retrofit packages across the existing Chicago-area residential building stock is predicted to reduce annual site energy use by 3.7 × 1016 J and yield approximately $280 million USD in annual energy savings.

  12. Efficient polymer white-light-emitting diodes with a single-emission layer of fluorescent polymer blend

    International Nuclear Information System (INIS)

    Niu Qiaoli; Xu Yunhua; Jiang Jiaxing; Peng Junbiao; Cao Yong

    2007-01-01

    Efficient polymer white-light-emitting diodes (WPLEDs) have been fabricated with a single layer of fluorescent polymer blend. The device structure consists of ITO/PEDOT/PVK/emissive layer/Ba/Al. The emissive layer is a blend of poly(9,9-dioctylfluorene) (PFO), phenyl-substituted PPV derivative (P-PPV) and a copolymer of 9,9-dioctylfluorene and 4,7-di(4-hexylthien-2-yl)-2,1,3-benzothiadiazole (PFO-DHTBT), which, respectively, emits blue, green and red light. The emission of pure and efficient white light was implemented by tuning the blend weight ratio of PFO: P-PPV: PFO-DHTBT to 96:4:0.4. The maximum current efficiency and luminance are, respectively, 7.6 cd/A at 6.7 V and 11930 cd/m 2 at 11.2 V. The CIE coordinates of white-light emission were stable with the drive voltages

  13. Development of a UAV-mounted Light Source for Fluorescence Detector Calibration of the Telescope Array Experiment

    Science.gov (United States)

    Hayashi, Motoki; Tameda, Yuichiro; Tomida, Takayuki; Tsunesada, Yoshiki; Seki, Terutsugu; Saito, Yoshinori

    We are developing a unmanned aerial vehicle (UAV), which is called "Opt-copter", carrying a calibrated light source for fluorescence detector (FD) calibration of the Telescope Array (TA) experiment. Opt-copter is equipped with a high accuracy GPS device and a LED light source in the shape of a dodecahedron. A positioning accuracy of the GPS mounted on the UAV is 0.1 m, which meets the requirement for the calibration of the FDs at the distance of 100 m. The light source consists of 12 UV LEDs attached on each side of the dodecahedron, and it is covered with a spherical diffuser to improve the spatial uniformity of the light intensity. We report the status of Opt-copter development and the results of its test at the TA site.

  14. The evaluation of retrofit measures in a tall residential building

    Energy Technology Data Exchange (ETDEWEB)

    Abraham, M.M.; McLain, H.A.

    1995-07-01

    As part of a joint demonstration effort involving the US Department of Energy (DOE), the US Department of Housing and Urban Development (HUD), Boston Edison Company (BECo), and the Chelsea Housing Authority, Oak Ridge National Laboratory (ORNL) participated in the evaluation of energy and demand saving retrofits for a tall residential building located in Boston. The thirteen story all-electric building underwent window, lighting, and control renovations in December, 1992. annual energy consumption was reduced by 15% and peak demand fell by 17%. Hourly should building consumption data were available for the comparison of pre- and post- conditions and for calibration of a DOE-2.1D simulation model. The analysis found the window retrofit accounted for 90% of total energy savings and 95% of average demand savings, due to reductions in both conduction and infiltration. Benefits from lighting retrofits were low in cooling months and negligible in winter months due to the increase in the demand for electric resistance heating which was proportional to the reduction in lighting capacity. Finally, the simulation model verified that heating system controls had not been used as intended, and that the utility rate structure would not allow cost savings from the original control strategy. These results and other interesting lessons learned are presented.

  15. University of Maryland Wall Washer Retrofit - LED Modules Replace Halogen Lamps in a Performing Arts Center

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, Andrea M. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Abell, Thomas C. [Univ. of Maryland, College Park, MD (United States); Perrin, Tess E. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-08-03

    The University of Maryland (UMD) began retrofitting halogen wall washers in the Clarice Smith Performing Arts Center (CSPAC) in April 2014. The U.S. Department of Energy (DOE) Solid-State Lighting (SSL) GATEWAY program documented this process through the final installation in March 2015, summarized in this report. The wall washers illuminate hallways lining the atrium, providing task illuminance for transitioning between spaces and visual interest to the atrium boundaries. The main goals of the retrofit were to maintain the visual appearance of the space while reducing maintenance costs – energy savings was considered an additional benefit by UMD Facilities Management. UMD Facilities Management is pleased with the results of this retrofit, and continues to initiate LED retrofit projects across the UMD campus.

  16. LED-FISH: Fluorescence microscopy based on light emitting diodes for the molecular analysis of Her-2/neu oncogene amplification

    Directory of Open Access Journals (Sweden)

    Vollmer Ekkehard

    2008-12-01

    Full Text Available Abstract Light emitting diodes (LED, which are available as small monochromatic light sources with characteristic features such as maximum illumination power combined with minimum energy consumption and extremely long lifespan have already proved as a highly potential low-cost alternative for specific diagnostic applications in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the most reliable evaluation of Her-2/neu (c-erbB2 gene amplification, which has been established in the last few years for routine diagnosis in clinical pathology as determinant towards Herceptin-based treatment of patients with breast cancer, is based on fluorescence in situ hybridization (FISH and corresponding high priced fluorescence equipment. In order to test the possibility to utilize the advantages of low-cost LED technology on FISH analysis of c-erbB2 gene expression for routine diagnostic purposes, the applicability of a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER* LED Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination with a conventional 100W mercury vapor lamp. Both microscopes were fitted with the same Quicam FAST CCD digital camera to unequivocally compare the quality of the captured images. C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. The Her2/neu gene signals (green were identifiable in the tumor cells in all cases and images of equal quality were captured under almost identical conditions by 480 nm (blue LED module equipped standard Axiostar microscope as compared to conventional fluorescence microscopy. In this first attempt, these monochromatic LED elements proved in principle to be suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own

  17. Advanced Catalytic Hydrogenation Retrofit Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Reinaldo M. Machado

    2002-08-15

    Industrial hydrogenation is often performed using a slurry catalyst in large stirred-tank reactors. These systems are inherently problematic in a number of areas, including industrial hygiene, process safety, environmental contamination, waste production, process operability and productivity. This program proposed the development of a practical replacement for the slurry catalysts using a novel fixed-bed monolith catalyst reactor, which could be retrofitted onto an existing stirred-tank reactor and would mitigate many of the minitations and problems associated with slurry catalysts. The full retrofit monolith system, consisting of a recirculation pump, gas/liquid ejector and monolith catalyst, is described as a monolith loop reactor or MLR. The MLR technology can reduce waste and increase raw material efficiency, which reduces the overall energy required to produce specialty and fine chemicals.

  18. Bay Ridge Gardens - Mixed Humid Affordable Multifamily Housing Deep Energy Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Lyons, James [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Moore, Mike [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Thompson, Margo [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States)

    2013-08-01

    Under this project, Newport Partners (as part of the BA-PIRC research team) evaluated the installation, measured performance, and cost effectiveness of efficiency upgrade measures for a tenant-in-place deep energy retrofit (DER) at the Bay Ridge multifamily development in Annapolis, Maryland. This report summarizes system commissioning, short-term test results, utility bill data analysis, and analysis of real-time data collected over a one-year period after the retrofit was complete. The Bay Ridge project is comprised of a "base scope" retrofit which was estimated to achieve a 30%+ savings (relative to pre-retrofit) on 186 apartments, and a "DER scope" which was estimated to achieve 50% savings (relative to pre-retrofit) on a 12-unit building. A wide range of efficiency measures was applied to pursue this savings target for the DER building, including improvements/replacements of mechanical equipment and distribution systems, appliances, lighting and lighting controls, the building envelope, hot water conservation measures, and resident education. The results of this research build upon the current body of knowledge of multifamily retrofits. Towards this end, the research team has collected and generated data on the selection of measures, their estimated performance, their measured performance, and risk factors and their impact on potential measures.

  19. Adaptation of photosystem II to high and low light in wild-type and triazine-resistant Canola plants: analysis by a fluorescence induction algorithm

    NARCIS (Netherlands)

    Rensen, van J.J.S.; Vredenberg, W.J.

    2011-01-01

    Plants of wild-type and triazine-resistant Canola (Brassica napus L.) were exposed to very high light intensities and after 1 day placed on a laboratory table at low light to recover, to study the kinetics of variable fluorescence after light, and after dark-adaptation. This cycle was repeated

  20. Review of organic light-emitting diodes with thermally activated delayed fluorescence emitters for energy-efficient sustainable light sources and displays

    Science.gov (United States)

    Volz, Daniel

    2016-04-01

    Thermally activated delayed fluorescence (TADF) is an emerging hot topic. Even though this photophysical mechanism itself has been described more than 50 years ago and optoelectronic devices with organic matter have been studied, improved, and even commercialized for decades now, the realization of the potential of TADF organic light-emitting diodes (OLEDs) happened only recently. TADF has been proven to be an attractive and very efficient alternative for phosphorescent materials, such as dopants in OLEDs, light-emitting electrochemical cells as well as potent emitters for chemiluminescence. In this review, the TADF concept is introduced in terms that are also understandable for nonchemists. The basic concepts behind this mechanism as well as state-of-the-art examples are discussed. In addition, the future economic impact, especially for the lighting and display market, is addressed here. We conclude that TADF materials are especially helpful to realize efficient, durable deep blue and white displays.

  1. Existing Whole-House Solutions Case Study: Retrofitting a 1960s Split-Level Cold-Climate Home

    Energy Technology Data Exchange (ETDEWEB)

    Puttagunta, S.

    2015-08-01

    National programs such as Home Performance with ENERGY STAR® and numerous other utility air sealing programs have brought awareness to homeowners of the benefits of energy efficiency retrofits. Yet, these programs tend to focus on the low-hanging fruit: air-sealing the thermal envelope and ductwork where accessible, switch to efficient lighting, and low-flow fixtures. At the other end of the spectrum, deep-energy retrofit programs are also being encouraged by various utilities across the country. While deep energy retrofits typically seek 50% energy savings, they are often quite costly and most applicable to gut-rehab projects. A significant potential for lowering energy usage in existing homes lies between the low hanging fruit and deep energy retrofit approaches - retrofits that save approximately 30% in energy over the existing conditions.

  2. Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy

    Energy Technology Data Exchange (ETDEWEB)

    McConnell, Gail [Centre for Biophotonics, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR (United Kingdom)

    2005-08-07

    Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.

  3. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L.

    Science.gov (United States)

    Hazrati, Saeid; Tahmasebi-Sarvestani, Zeinolabedin; Modarres-Sanavy, Seyed Ali Mohammad; Mokhtassi-Bidgoli, Ali; Nicola, Silvana

    2016-09-01

    Aloe vera L. is one of the most important medicinal plants in the world. In order to determine the effects of light intensity and water deficit stress on chlorophyll (Chl) fluorescence and pigments of A. vera, a split-plot in time experiment was laid out in a randomized complete block design with four replications in a research greenhouse. The factorial combination of three light intensities (50, 75 and 100% of sunlight) and four irrigation regimes (irrigation after depleting 20, 40, 60 and 80% of soil water content) were considered as main factors. Sampling time was considered as sub factor. The first, second and third samplings were performed 90, 180 and 270 days after imposing the treatments, respectively. The results demonstrated that the highest light intensity and the severe water stress decreased maximum fluorescence (Fm), variable fluorescence (Fv)/Fm, quantum yield of PSII photochemistry (ФPSII), Chl and photochemical quenching (qP) but increased non-photochemical quenching (NPQ), minimum fluorescence (F0) and Anthocyanin (Anth). Additionally, the highest Fm, Fv/Fm, ФPSII and qP and the lowest NPQ and F0 were observed when 50% of sunlight was blocked and irrigation was done after 40% soil water depletion. Irradiance of full sunlight and water deficit stress let to the photoinhibition of photosynthesis, as indicated by a reduced quantum yield of PSII, ФPSII, and qP, as well as higher NPQ. Thus, chlorophyll florescence measurements provide valuable physiological data. Close to half of total solar radiation and irrigation after depleting 40% of soil water content were selected as the most efficient treatments. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  4. Effect of light-emitting diode (LED) vs. fluorescent (FL) lighting on laying hens in aviary hen houses: Part 2 - Egg quality, shelf-life and lipid composition.

    Science.gov (United States)

    Long, H; Zhao, Y; Xin, H; Hansen, H; Ning, Z; Wang, T

    2016-01-01

    In this 60-wk study, egg quality, egg shelf-life, egg cholesterol content, total yolk lipids, and yolk fatty acid composition of eggs produced by Dekalb white laying hens in commercial aviary houses with either light-emitting diode (LED) or fluorescent (FL) lighting were compared. All parameters were measured at 27, 40, and 60 wk of age, except for egg shelf-life, which was compared at 50 wk of age. The results showed that, compared to the FL regimen, the LED regimen resulted in higher egg weight, albumen height, and albumen weight at 27 wk of age, thicker shells at 40 wk of age, but lower egg weight at 60 wk of age. Egg quality change was similar between the lighting regimens during the 62-d egg storage study, indicating that LED lighting did not influence egg shelf-life. Eggs from both lighting regimens had similar cholesterol content. However, cholesterol concentration of the yolk (15.9 to 21.0 mg cholesterol/g wet weight yolk) observed in this study was higher than that of United States Department of Agriculture (USDA) database (10.85 mg/g). No significant differences in total lipids or fatty acid composition of the yolks were detected between the two lighting regimens. © 2015 Poultry Science Association Inc.

  5. Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein

    DEFF Research Database (Denmark)

    Østergaard, H.; Henriksen, A.; Hansen, Flemming G.

    2001-01-01

    To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide bond between the two cysteines was fully reversible and resulted in a >2-fold decrease...... in the intrinsic fluorescence. Inter conversion between the two redox states could thus be followed in vitro as well as in vivoby non- invasive fluorimetric measurements. The 1.5 Angstrom crystal structure of the oxidized protein revealed a disulfide bond- induced distortion of the beta -barrel, as well...... as a structural reorganization of residues in the immediate chromophore environment. By combining this information with spectroscopic data, we propose a detailed mechanism accounting for the observed redox state-dependent fluorescence. The redox potential of the cysteine couple was found to be within...

  6. A Review of the Reflector Compact Fluorescent Lights Technology Procurement Program: Conclusions and Results

    Energy Technology Data Exchange (ETDEWEB)

    Sandahl, Linda J.; Gilbride, Theresa L.; Ledbetter, Marc R.; McCullough, Jeffrey J.

    2008-05-19

    This report describes a project sponsored by the U.S. Department of Energy (DOE) and implemented by the Pacific Northwest National Laboratory (PNNL), from 2000 to 2007 to improve the performance of reflector type (R-lamp) compact fluorescent lamps (CFLs) and increase their availability throughout the United States by means of a technology development and procurement strategy. In 2000, at the request of the U.S. Department of Energy’s Emerging Technologies Program and its predecessors, the Pacific Northwest National Laboratory undertook a technology procurement seeking R-CFLs that were specifically designed for use in ICAT recessed can fixtures and that met other minimum performance criteria including minimum light output and size restrictions (to ensure they fit in standard residential recessed cans). The technology procurement included two phases. In Phase I, requests for proposals (RFPs) were issued in October 2002 and five manufacturers responded with 12 lamp models. Eight of these models met the minimum requirements and passed the 6-hour short-term test in a simulated ICAT environment. These eight models were subjected to long-term tests of 6,000 or more hours in a simulated ICAT environment. Three of these models passed the short- and long-term tests and were promoted through the program website (www.pnl.gov/rlamps), press releases, and fliers. To increase the number of qualifying models, a second RFP was issued in June 2005. In April 2007, DOE announced that 16 reflector CFL (R-CFL) models by four manufacturers had met all the minimum requirements of Phase 2 of the R-CFL Technology Innovation Competition. PNNL developed both the criteria and the test apparatus design for Elevated Temperature Life Testing (ETLT), which has been included by DOE in its draft ENERGY STAR specifications for the reflector category of CFLs. PNNL promoted the winning lamps through a program website, press releases, and fliers as well as through program partners. PNNL also helped

  7. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.

    Science.gov (United States)

    Hofmann, Michael; Eggeling, Christian; Jakobs, Stefan; Hell, Stefan W

    2005-12-06

    Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated depletion of the marker's fluorescent state by stimulated emission, but this approach requires picosecond laser pulses of GW/cm2 intensity. Here, we demonstrate the surpassing of the diffraction barrier in fluorescence microscopy with illumination intensities that are eight orders of magnitude smaller. The subdiffraction resolution results from reversible photoswitching of a marker protein between a fluorescence-activated and a nonactivated state, whereby one of the transitions is accomplished by means of a spatial intensity distribution featuring a zero. After characterizing the switching kinetics of the used marker protein asFP595, we demonstrate the current capability of this RESOLFT (reversible saturable optical fluorescence transitions) type of concept to resolve 50-100 nm in the focal plane. The observed resolution is limited only by the photokinetics of the protein and the perfection of the zero. Our results underscore the potential to finally achieve molecular resolution in fluorescence microscopy by technical optimization.

  8. Comparison of hexaminolevulinate based flexible and rigid fluorescence cystoscopy with rigid white light cystoscopy in bladder cancer: results of a prospective Phase II study.

    NARCIS (Netherlands)

    Witjes, J.A.; Moonen, P.M.J.; Heijden, A.G. van der

    2005-01-01

    INTRODUCTION AND OBJECTIVE: Several studies have shown that rigid fluorescence cystoscopy (RFC) with hexaminolevulinate (HAL) is superior to standard rigid white light (RWLC) cystoscopy in diagnosing bladder tumours, with a clinically relevant impact on the patient's management. These studies,

  9. Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein

    DEFF Research Database (Denmark)

    Østergaard, H.; Henriksen, A.; Hansen, Flemming G.

    2001-01-01

    To visualize the formation of disulfide bonds in living cells, a pair of redox-active cysteines was introduced into the yellow fluorescent variant of green fluorescent protein. Formation of a disulfide bond between the two cysteines was fully reversible and resulted in a >2-fold decrease...... the physiological range for redox-active cysteines. In the cytoplasm of Escherichia coli, the protein was a sensitive probe for the redox changes that occur upon disruption of the thioredoxin reductive pathway....... in the intrinsic fluorescence. Inter conversion between the two redox states could thus be followed in vitro as well as in vivoby non- invasive fluorimetric measurements. The 1.5 Angstrom crystal structure of the oxidized protein revealed a disulfide bond- induced distortion of the beta -barrel, as well...

  10. Total reflection X-ray fluorescence analysis of light elements with synchrotron radiation and special X-ray tubes

    International Nuclear Information System (INIS)

    Streli, C.; Wobrauschek, P.; Bauer, V.; Kregsamer, P.; Goergl, R.; Pianetta, P.; Ryon, R.; Pahlke, S.; Fabry, L.

    1997-01-01

    Total reflection X-ray fluorescence analysis (TXRF) of light elements, such as C, O and Al (atomic numbers 5-13) generally has poor sensitivity and detection limits due to poor excitation and low fluorescent yields. Special excitation sources are necessary to compensate for these physical limitations. Synchrotron radiation is the ideal source for TXRF due to its high intensity and wide spectral range extending into the low energy region required for light elements. For more routine use, special X-ray tubes can be constructed. Experiments have been performed at the Standford Synchrotron Radiation Laboratory (SSRL) using beamline III-4, which is specially designed for the sue of low energy photons. Light elements on Si wafers have been analyzed, leading to detection limits below 100 fg for Na, Mg and Al, which corresponds to about 10 9 atoms. A new vacuum chamber is introduced meeting the requirements of wafer handling without the risk of contamination and offering the possibility of scanning a certain area of the wafer. Boron was detected on a wafer with 10 14 atoms cm -2 implanted in the surface layer. A special windowless X-ray tube with Mo, Al and Si as anode materials was also tested. With the optimization of anode geometry, beam path and excitation conditions, a detection limit of 5 pg (corresponds to 10 11 atoms) for Al was achieved. (Author)

  11. Total reflection X-ray fluorescence analysis of light elements with synchrotron radiation and special X-ray tubes

    Energy Technology Data Exchange (ETDEWEB)

    Streli, C.; Wobrauschek, P.; Bauer, V.; Kregsamer, P.; Goergl, R. [Atominstitut der Oesterreichischen Universitaeten, Wien (Austria); Pianetta, P. [Stanford Synchrotron Radiation Lab. (Canada); Ryon, R. [Lawrence Livermore National Lab. CA (United States); Pahlke, S.; Fabry, L. [Wacker Siltronic AG, Burghausen (Germany)

    1997-06-20

    Total reflection X-ray fluorescence analysis (TXRF) of light elements, such as C, O and Al (atomic numbers 5-13) generally has poor sensitivity and detection limits due to poor excitation and low fluorescent yields. Special excitation sources are necessary to compensate for these physical limitations. Synchrotron radiation is the ideal source for TXRF due to its high intensity and wide spectral range extending into the low energy region required for light elements. For more routine use, special X-ray tubes can be constructed. Experiments have been performed at the Standford Synchrotron Radiation Laboratory (SSRL) using beamline III-4, which is specially designed for the sue of low energy photons. Light elements on Si wafers have been analyzed, leading to detection limits below 100 fg for Na, Mg and Al, which corresponds to about 10{sup 9}atoms. A new vacuum chamber is introduced meeting the requirements of wafer handling without the risk of contamination and offering the possibility of scanning a certain area of the wafer. Boron was detected on a wafer with 10{sup 14} atoms cm{sup -2} implanted in the surface layer. A special windowless X-ray tube with Mo, Al and Si as anode materials was also tested. With the optimization of anode geometry, beam path and excitation conditions, a detection limit of 5 pg (corresponds to 10{sup 11} atoms) for Al was achieved. (Author).

  12. csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm.

    Science.gov (United States)

    Chang, Bo-Jui; Perez Meza, Victor Didier; Stelzer, Ernst H K

    2017-05-09

    Light-sheet-based fluorescence microscopy (LSFM) features optical sectioning in the excitation process. It minimizes fluorophore bleaching as well as phototoxic effects and provides a true axial resolution. The detection path resembles properties of conventional fluorescence microscopy. Structured illumination microscopy (SIM) is attractive for superresolution because of its moderate excitation intensity, high acquisition speed, and compatibility with all fluorophores. We introduce SIM to LSFM because the combination pushes the lateral resolution to the physical limit of linear SIM. The instrument requires three objective lenses and relies on methods to control two counterpropagating coherent light sheets that generate excitation patterns in the focal plane of the detection lens. SIM patterns with the finest line spacing in the far field become available along multiple orientations. Flexible control of rotation, frequency, and phase shift of the perfectly modulated light sheet are demonstrated. Images of beads prove a near-isotropic lateral resolution of sub-100 nm. Images of yeast endoplasmic reticulum show that coherent structured illumination (csi) LSFM performs with physiologically relevant specimens.

  13. Influence of the light-curing unit, storage time and shade of a dental composite resin on the fluorescence

    Science.gov (United States)

    Queiroz, R. S.; Bandéca, M. C.; Calixto, L. R.; Gaiao, U.; Cuin, A.; Porto-Neto, S. T.

    2010-07-01

    The aim of this study was to determine the influence of three light-curing units, storage times and colors of the dental composite resin on the fluorescence. The specimens (diameter 10.0 ± 0.1 mm, thickness 1.0 ± 0.1 mm) were made using a stainless steel mold. The mold was filled with the microhybrid composite resin and a polyethylene film covered each side of the mold. After this, a glass slide was placed on the top of the mold. To standardize the top surface of the specimens a circular weight (1 kg) with an orifice to pass the light tip of the LCU was placed on the top surface and photo-activated during 40 s. Five specimens were made for each group. The groups were divided into 9 groups following the LCUs (one QTH and two LEDs), storage times (immediately after curing, 24 hours, 7 and 30 days) and colors (shades: A2E, A2D, and TC) of the composite resin. After photo-activation, the specimens were storage in artificial saliva during the storage times proposed to each group at 37°C and 100% humidity. The analysis of variance (ANOVA) and Tukey’s posthoc tests showed no significant difference between storage times (immediately, 24 hours and 30 days) ( P > 0.05). The means of fluorescence had difference significant to color and light-curing unit used to all period of storage ( P 0.05).

  14. "DNA Origami Traffic Lights" with a Split Aptamer Sensor for a Bicolor Fluorescence Readout.

    Science.gov (United States)

    Walter, Heidi-Kristin; Bauer, Jens; Steinmeyer, Jeannine; Kuzuya, Akinori; Niemeyer, Christof M; Wagenknecht, Hans-Achim

    2017-04-12

    A split aptamer for adenosine triphosphate (ATP) was embedded as a recognition unit into two levers of a nanomechanical DNA origami construct by extension and modification of selected staple strands. An additional optical module in the stem of the split aptamer comprised two different cyanine-styryl dyes that underwent an energy transfer from green (donor) to red (acceptor) emission if two ATP molecules were bound as target molecule to the recognition module and thereby brought the dyes in close proximity. As a result, the ATP as a target triggered the DNA origami shape transition and yielded a fluorescence color change from green to red as readout. Conventional atomic force microscopy (AFM) images confirmed the topology change from the open form of the DNA origami in the absence of ATP into the closed form in the presence of the target molecule. The obtained closed/open ratios in the absence and presence of target molecules tracked well with the fluorescence color ratios and thereby validated the bicolor fluorescence readout. The correct positioning of the split aptamer as the functional unit farthest away from the fulcrum of the DNA origami was crucial for the aptasensing by fluorescence readout. The fluorescence color change allowed additionally to follow the topology change of the DNA origami aptasensor in real time in solution. The concepts of fluorescence energy transfer for bicolor readout in a split aptamer in solution, and AFM on surfaces, were successfully combined in a single DNA origami construct to obtain a bimodal readout. These results are important for future custom DNA devices for chemical-biological and bioanalytical purposes because they are not only working as simple aptamers but are also visible by AFM on the single-molecule level.

  15. Quantifying the Financial Benefits of Multifamily Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Philbrick, D. [Elevate Energy, Chicago, IL (United States); Scheu, R. [Elevate Energy, Chicago, IL (United States); Brand, L. [Elevate Energy, Chicago, IL (United States)

    2016-01-01

    The U.S. Department of Energy’s Building America research team Partnership for Advanced Residential Retrofit analyzed building, energy, and financial program data as well as other public and private data to examine the relationship between energy-efficiency retrofits and financial performance on three levels: building, city, and community.

  16. Prologis Rolls Out Warehouse Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-03-01

    Prologis partnered with the U.S. Department of Energy (DOE) to develop and implement solutions to retrofit existing buildings to reduce annual energy consumption by at least 30% versus pre-retrofit energy use as part of DOE’s Commercial Building Partnership (CBP) program.

  17. Multi-channel LED light source for fluorescent agent aided minimally invasive surgery.

    Science.gov (United States)

    Ren, Jiacheng; Venugopalan, Janani; Xu, Jian; Kairdolf, Brad; Durfee, Robert; Wang, May D

    2014-01-01

    Cancer is one of the most common and deadly diseases around the world. Amongst all the different treatments of cancer such as surgery, chemotherapy and radiation therapy, surgical resection is the most effective. Successful surgeries greatly rely on the detection of the accurate tumor size and location, which can be enhanced by contrast agents. Commercial endoscope light sources, however, offer only white light illumination. In this paper, we present the development of a LED endoscope light source that provides 2 light channels plus white light to help surgeons to detect a clear tumor margin during minimally invasive surgeries. By exciting indocyanine green (ICG) and 5-Aminolaevulinic acid (ALA)-induced protoporphyrin IX (PPIX), the light source is intended to give the user a visible image of the tumor margin. This light source is also portable, easy to use and costs less than $300 to build.

  18. Surgical instrument biocontaminant fluorescence detection in ambient lighting conditions for hospital reprocessing and sterilization department (Conference Presentation)

    Science.gov (United States)

    Baribeau, François; Bubel, Annie; Dumont, Guillaume; Vachon, Carl; Lépine, André; Rochefort, Stéphane; Massicotte, Martin; Buteau-Vaillancourt, Louis; Gallant, Pascal; Mermut, Ozzy

    2017-03-01

    Hospitals currently rely on simple human visual inspection for assessing cleanliness of surgical instruments. Studies showed that surgical site infections are in part attributed to inadequate cleaning of medical devices. Standards groups recognize the need to objectively quantify the amount of residues on surgical instruments and establish guidelines. We developed a portable technology for the detection of contaminants on surgical instruments through fluorescence following cleaning. Weak fluorescence signals are usually detected in the obscurity only with the lighting of the excitation source. The key element of this system is that it works in ambient lighting conditions, a requirement to not disturb the normal workflow of hospital reprocessing facilities. A biocompatible fluorescent dye is added to the detergent and labels the proteins of organic residues. It is resistant to the harsh environment in a washer-disinfector. Two inspection devices have been developed with a 488nm laser as the excitation source: a handheld scanner and a tabletop station using spectral-domain and time-domain ambient light cancellation schemes. The systems are eye safe and equipped with image processing and interfacing software to provide visual or audible warnings to the operator based on a set of adjustable signal thresholds. Micron-scale residues are detected by the system which can also evaluate soil size and mass. Unlike swabbing, it can inspect whole tools in real-time. The technology has been validated in an independent hospital decontamination research laboratory. It also has potential applications in the forensics, agro-food, and space fields. Technical aspects and results will be presented and discussed.

  19. Leaf Morphology, Photosynthetic Performance, Chlorophyll Fluorescence, Stomatal Development of Lettuce (Lactuca sativa L.) Exposed to Different Ratios of Red Light to Blue Light.

    Science.gov (United States)

    Wang, Jun; Lu, Wei; Tong, Yuxin; Yang, Qichang

    2016-01-01

    Red and blue light are both vital factors for plant growth and development. We examined how different ratios of red light to blue light (R/B) provided by light-emitting diodes affected photosynthetic performance by investigating parameters related to photosynthesis, including leaf morphology, photosynthetic rate, chlorophyll fluorescence, stomatal development, light response curve, and nitrogen content. In this study, lettuce plants (Lactuca sativa L.) were exposed to 200 μmol⋅m(-2)⋅s(-1) irradiance for a 16 h⋅d(-1) photoperiod under the following six treatments: monochromatic red light (R), monochromatic blue light (B) and the mixture of R and B with different R/B ratios of 12, 8, 4, and 1. Leaf photosynthetic capacity (A max) and photosynthetic rate (P n) increased with decreasing R/B ratio until 1, associated with increased stomatal conductance, along with significant increase in stomatal density and slight decrease in stomatal size. P n and A max under B treatment had 7.6 and 11.8% reduction in comparison with those under R/B = 1 treatment, respectively. The effective quantum yield of PSII and the efficiency of excitation captured by open PSII center were also significantly lower under B treatment than those under the other treatments. However, shoot dry weight increased with increasing R/B ratio with the greatest value under R/B = 12 treatment. The increase of shoot dry weight was mainly caused by increasing leaf area and leaf number, but no significant difference was observed between R and R/B = 12 treatments. Based on the above results, we conclude that quantitative B could promote photosynthetic performance or growth by stimulating morphological and physiological responses, yet there was no positive correlation between P n and shoot dry weight accumulation.

  20. Effect of Different Light Qualities on Growth, Pigment Content, Chlorophyll Fluorescence, and Antioxidant Enzyme Activity in the Red Alga Pyropia haitanensis (Bangiales, Rhodophyta).

    Science.gov (United States)

    Wu, Huanyang

    2016-01-01

    Spectral light changes evoke different morphogenetic and photosynthetic responses that can vary among different algae species. The aim of this study is to investigate the photosynthetic characteristics of the red macroalgae grown under different spectrum environments. In this study, Pyropia haitanensis were cultured under blue, red, and green LED and fluorescent tubes light. The growth rate, photopigment composition, chlorophyll fluorescence, and antioxidative enzymes activities in different light spectrums were investigated. The results revealed that growth rate was significantly higher in the thalli grown under blue, green, and fluorescent tubes light. Contents of Chl a and phycobiliprotein in red light were lower among all the growth conditions. Furthermore, a striking increase in SOD and CAT activity was observed in red light treatment along with the NPQ increase. The results revealed that the photosynthetic efficiency and increased growth rate of P. haitanensis benefitted from light spectrums such as blue, green, and fluorescent tubes light by pigment composition and photochemical efficiency manipulation, whereas red light has disadvantageous effects. Accordingly, the results for improving quality and the economic yield of algae species in some extent and the combination of different wavelengths could allow better economic resource exploitation.

  1. Effect of Different Light Qualities on Growth, Pigment Content, Chlorophyll Fluorescence, and Antioxidant Enzyme Activity in the Red Alga Pyropia haitanensis (Bangiales, Rhodophyta

    Directory of Open Access Journals (Sweden)

    Huanyang Wu

    2016-01-01

    Full Text Available Spectral light changes evoke different morphogenetic and photosynthetic responses that can vary among different algae species. The aim of this study is to investigate the photosynthetic characteristics of the red macroalgae grown under different spectrum environments. In this study, Pyropia haitanensis were cultured under blue, red, and green LED and fluorescent tubes light. The growth rate, photopigment composition, chlorophyll fluorescence, and antioxidative enzymes activities in different light spectrums were investigated. The results revealed that growth rate was significantly higher in the thalli grown under blue, green, and fluorescent tubes light. Contents of Chl a and phycobiliprotein in red light were lower among all the growth conditions. Furthermore, a striking increase in SOD and CAT activity was observed in red light treatment along with the NPQ increase. The results revealed that the photosynthetic efficiency and increased growth rate of P. haitanensis benefitted from light spectrums such as blue, green, and fluorescent tubes light by pigment composition and photochemical efficiency manipulation, whereas red light has disadvantageous effects. Accordingly, the results for improving quality and the economic yield of algae species in some extent and the combination of different wavelengths could allow better economic resource exploitation.

  2. A Fluorescence Light-Up Ag Nanocluster Probe that Discriminates Single-Nucleotide Variants by Emission Color

    OpenAIRE

    Yeh, Hsin-Chih; Sharma, Jaswinder; Shih, Ie-Ming; Vu, Dung M.; Martinez, Jennifer S.; Werner, James H.

    2012-01-01

    Rapid and precise screening of small genetic variations, such as single-nucleotide polymorphisms (SNPs), among an individual’s genome is still an unmet challenge at point-of-care settings. One crucial step towards this goal is the development of discrimination probes that require no enzymatic reaction and are easy to use. Here we report a new type of fluorescent molecular probe, termed a chameleon NanoCluster Beacon (cNCB), that lights up into different colors upon binding SNP targets. NanoCl...

  3. The effect of pressure on the fluorescence of a new type of light-emitting material

    CERN Document Server

    Zhong Bo; He Li Ming; Li Yi; Bai Feng Lian; Yang Guo Qiang

    2002-01-01

    The fluorescence properties of two nitrogen-containing poly-(phenylene vinylene)-related copolymers were investigated under high pressure. Because of the large difference in interchain interaction between these two copolymers, the emission states are different from each other. Under the perturbation introduced by compression, the emission intensity exhibits different trends of change.

  4. Broadband Antireflection and Light Extraction Enhancement in Fluorescent SiC with Nanodome Structures

    DEFF Research Database (Denmark)

    Ou, Yiyu; Zhu, Xiaolong; Jokubavicius, Valdas

    2014-01-01

    We demonstrate a time-efficient and low-cost approach to fabricate Si3N4 coated nanodome structures in fluorescent SiC. Nanosphere lithography is used as the nanopatterning method and SiC nanodome structures with Si3N4 coating are formed via dry etching and thin film deposition process. By using...... in the future....

  5. The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy

    NARCIS (Netherlands)

    Singh, A.P.; Krieger, J.W.; Buchholz, J.; Charbon, E.; Langowski, J.; Wohland, T.

    2013-01-01

    Single plane illumination microscopy based fluorescence correlation spectroscopy (SPIM-FCS) is a new method for imaging FCS in 3D samples, providing diffusion coefficients, transport, flow velocities and concentrations in an imaging mode. SPIM-FCS records correlation functions over a whole plane in

  6. Body Temperature Controlled Optical and Thermal Information Storage Light Scattering Display with Fluorescence Effect and High Mechanical Strength.

    Science.gov (United States)

    Chen, Si; Tong, Xiaoqian; He, Huiwen; Ma, Meng; Shi, Yanqin; Wang, Xu

    2017-04-05

    A kind of body temperature controlled optical and thermal information storage light scattering display based on super strong liquid crystalline physical gel with special "loofah-like gel network" was successfully prepared. Such liquid crystal (LC) gel was obtained by mixing a dendritic gelator (POSS-G1-BOC), an azobenzene compound (2Azo2), and a phosphor tethered liquid crystalline host (5CB), which could show its best contrast ratio at around human body temperature under UV light because of the phosphor's fluorescence effect. The gel also has quite strong mechanical strength, which could be used in wearable device field especially under sunlight, even under the forcing conditions as harsh as being centrifuged for 10 min at the speed of 2000 r/min. The whole production process of such a display is quite simple and could lead to displays at any size through noncontact writing. We believe it will have wide applications in the future.

  7. Thermally Activated Delayed Fluorescence Emitters for Deep Blue Organic Light Emitting Diodes: A Review of Recent Advances

    Directory of Open Access Journals (Sweden)

    Thanh-Tuân Bui

    2018-03-01

    Full Text Available Organic light-emitting diodes offer attractive perspectives for the next generation display and lighting technologies. The potential is huge and the list of potential applications is almost endless. So far, blue emitters still suffer from noticeably inferior electroluminescence performances in terms of efficiency, lifespan, color quality, and charge injection/transport when compared to that of the other colors. Emitting materials matching the NTSC standard blue of coordinates (0.14, 0.08 are extremely rare and still constitutes the focus of numerous academic and industrial researches. In this context, we review herein the recent developments on highly emissive deep-blue thermally activated delayed fluorescence emitters that constitute the third-generation electroluminescent materials.

  8. Performance of a two-leaf light use efficiency model for mapping gross primary productivity against remotely sensed sun-induced chlorophyll fluorescence data.

    Science.gov (United States)

    Zan, Mei; Zhou, Yanlian; Ju, Weimin; Zhang, Yongguang; Zhang, Leiming; Liu, Yibo

    2018-02-01

    Estimating terrestrial gross primary production is an important task when studying the carbon cycle. In this study, the ability of a two-leaf light use efficiency model to simulate regional gross primary production in China was validated using satellite Global Ozone Monitoring Instrument - 2 sun-induced chlorophyll fluorescence data. The two-leaf light use efficiency model was used to estimate daily gross primary production in China's terrestrial ecosystems with 500-m resolution for the period from 2007 to 2014. Gross primary production simulated with the two-leaf light use efficiency model was resampled to a spatial resolution of 0.5° and then compared with sun-induced chlorophyll fluorescence. During the study period, sun-induced chlorophyll fluorescence and gross primary production simulated by the two-leaf light use efficiency model exhibited similar spatial and temporal patterns in China. The correlation coefficient between sun-induced chlorophyll fluorescence and monthly gross primary production simulated by the two-leaf light use efficiency model was significant (pproduction simulated by the two-leaf light use efficiency model were similar in spring and autumn in most vegetated regions, but dissimilar in winter and summer. The spatial variability of sun-induced chlorophyll fluorescence and gross primary production simulated by the two-leaf light use efficiency model was similar in spring, summer, and autumn. The proportion of spatial variations of sun-induced chlorophyll fluorescence and annual gross primary production simulated by the two-leaf light use efficiency model explained by ranged from 0.76 (2011) to 0.80 (2013) during the study period. Overall, the two-leaf light use efficiency model was capable of capturing spatial and temporal variations in gross primary production in China. However, the model needs further improvement to better simulate gross primary production in summer. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Proven Performance of Seven Cold Climate Deep Retrofit Homes

    Energy Technology Data Exchange (ETDEWEB)

    Osser, R.; Neuhauser, K.; Ueno, K.

    2012-06-01

    Seven test homes located in Massachusetts are examined within this report. The retrofit strategies of each home are presented along with a comparison of the pre- and post-retrofit airtightness achieved by the group. Pre- and post-retrofit utility bills were collected; energy models were used to estimate pre-retrofit energy use when bills were unavailable.

  10. Methodology of light response curves: application of chlorophyll fluorescence to microphytobenthic biofilms

    NARCIS (Netherlands)

    Herlory, O.; Richard, P.; Blanchard, G.F.

    2007-01-01

    The light response curve methodology for microphytobenthic biofilms was studied by comparing the two most usual approaches used in pulse amplitude modulated (PAM) fluorometry. The non-sequential light curve (N-SLC) method is characterized by independent measures of the photosynthetic activity across

  11. Immobilized/P25/DSAT and Immobilized/Kronos/DSAT on Photocatalytic Degradation of Reactive Red 4 Under Fluorescent Light

    Directory of Open Access Journals (Sweden)

    Azami M. S.

    2016-01-01

    Full Text Available In this work, photocatalytic degradation of Reactive Red 4 (RR4 using immobilized P25 and kronos were performed under fluorescent light sources. The photocatalysis activity for both catalysts was investigated under fluorescent lamp source which consist UV and Visible light. The effect of various parameters such as initial concentration, initial pH and strenght of immobilized plate were studied. The result showed that 90% of RR4 dye was degrade in 1 hr using immobilized/kronos/DSAT at 100 mg L-1 of RR4 dye while 81% degradation was achieved by immobilized/P25/DSAT at the same condition. The lowest pH showed the higher photocatalytic activity. Hence, the effect of dye concentration and pH on the photocatalysis study can be related with the behavior of environmental pollution. The low strength showed by immobilized/P25/DSAT where it remain 37 % as compared with strength of immobilized/kronos/DSAT (52 wt.%. For the future work, the polymer binder like Polyvinyl alcohol (PVA, Polyethylene glycol (PEG, and others polymers can be apply in immobilized study to overcome the strength problem.

  12. Diurnal changes in chlorophyll fluorescence and light utilization in Colocasia esculenta leaves grown in marshy waterlogged area

    International Nuclear Information System (INIS)

    Roy Chowdhury, S.; Ashwani Kumar; Sahoo, N.

    2009-01-01

    Diurnal cycle of chlorophyll fluorescence was evaluated in Colocasia esculenta grown in marshy land under sun or under shade. The sun leaves maintained higher electron transport rate (ETR) and steady state to initial fluorescence ratio (Fs/F0) than shade leaves. In spite of lower ETR, higher photochemical quenching (PQ) and effective quantum yield of photosystem 2 (PS 2) was evident in shade plants compared to plants exposed to higher irradiance. ETR increased linearly with increase in irradiance more under low irradiance (r2 = 0.84) compared to higher irradiance (r2 = 0.62). The maximum quantum yield of PS 2 (Fv/Fm) did not differ much in sun and shade leaves with the exception of midday when excess of light energy absorbed by plants under sun was thermally dissipated. Hence swamp taro plants adopted different strategies to utilize radiation under different irradiances. At higher irradiance, there was faster decline in proportion of open PS 2 centers (PQ) and excess light energy was dissipated through non-photochemical quenching. Under shade, absorbed energy was effectively utilized resulting in higher PS2

  13. Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.

    Science.gov (United States)

    Hall, Gordon H; Glerum, D Moira; Backhouse, Christopher J

    2016-02-01

    Electrophoretic separation of fluorescently end-labeled DNA after a PCR serves as a gold standard in genetic diagnostics. Because of their size and cost, instruments for this type of analysis have had limited market uptake, particularly for point-of-care applications. This might be changed through a higher level of system integration and lower instrument costs that can be realized through the use of LEDs for excitation and photodiodes for detection--if they provide sufficient sensitivity. Here, we demonstrate an optimized microchip electrophoresis instrument using polymeric fluidic chips with fluorescence detection of end-labeled DNA with a LOD of 0.15 nM of Alexa Fluor 532. This represents orders of magnitude improvement over previously reported instruments of this type. We demonstrate the system with an electrophoretic separation of two PCR products and their respective primers. We believe that this is the first LED-induced fluorescence microchip electrophoresis system with photodiode-based detection that could be used for standard applications of PCR and electrophoresis. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Pulsed lasers versus continuous light sources in capillary electrophoresis and fluorescence detection studies: Photodegradation pathways and models.

    Science.gov (United States)

    Boutonnet, Audrey; Morin, Arnaud; Petit, Pierre; Vicendo, Patricia; Poinsot, Véréna; Couderc, François

    2016-03-17

    Pulsed lasers are widely used in capillary electrophoresis (CE) studies to provide laser induced fluorescence (LIF) detection. Unfortunately pulsed lasers do not give linear calibration curves over a wide range of concentrations. While this does not prevent their use in CE/LIF studies, the non-linear behavior must be understood. Using 7-hydroxycoumarin (7-HC) (10-5000 nM), Tamra (10-5000 nM) and tryptophan (1-200 μM) as dyes, we observe that continuous lasers and LEDs result in linear calibration curves, while pulsed lasers give polynomial ones. The effect is seen with both visible light (530 nm) and with UV light (355 nm, 266 nm). In this work we point out the formation of byproducts induced by pulsed laser upon irradiation of 7-HC. Their separation by CE using two Zeta LIF detectors clearly shows that this process is related to the first laser detection. All of these photodegradation products can be identified by an ESI-/MS investigation and correspond to at least two 7HC dimers. By using the photodegradation model proposed by Heywood and Farnsworth (2010) and by taking into account the 7-HC results and the fact that in our system we do not have a constant concentration of fluorophore, it is possible to propose a new photochemical model of fluorescence in LIF detection. The model, like the experiment, shows that it is difficult to obtain linear quantitation curves with pulsed lasers while UV-LEDs used in continuous mode have this advantage. They are a good alternative to UV pulsed lasers. An application involving the separation and linear quantification of oligosaccharides labeled with 2-aminobezoic acid is presented using HILIC and LED (365 nm) induced fluorescence. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Fluorescent Silicon Carbide and its Applications in White Light-Emitting Diodes

    DEFF Research Database (Denmark)

    Ou, Yiyu

    light extraction efficiency are presented. White LEDs are the most promising techniques to replace the conventional lighting sources. A typical white LED consists of a Gallium Nitride (GaN) blue or Ultraviolet (UV) LED stack and a wavelengthconversion material. Silicon Carbide (SiC) has a wide optical...... materials usually suffer from the low light extraction efficiency due to the large refractive index difference between air and semiconductor interface. To ease this limitation, ARS have been widely applied on the semiconductor surface in LED and solar cell applications. This thesis has theoretically...

  16. Nordic campus retrofitting concepts - Scalable practices

    DEFF Research Database (Denmark)

    Eriksson, Robert; Nenonen, Suvi; Junghans, Antje

    2015-01-01

    were about the new ways of researching, collaborating and learning with the concepts of Living lab, Creative community for innovation and entrepreneurship and Network of learning hubs. The cases provided the first insights on retrofitting based on users’ changing needs and the requirements of more......Multidisciplinary collaboration and transformations in learning processes can be supported by activity-based campus retrofitting. The aim of this paper is to analyse the ongoing campus retrofitting processes at the three university campuses and to identify the elements of activity...

  17. Intraocular light scatter, reflections, fluorescence and absorption: what we see in the slit lamp.

    Science.gov (United States)

    van den Berg, Thomas J T P

    2018-01-01

    Much knowledge has been collected over the past 20 years about light scattering in the eye- in particular in the eye lens- and its visual effect, called straylight. It is the purpose of this review to discuss how these insights can be applied to understanding the slit lamp image. The slit lamp image mainly results from back scattering, whereas the effects on vision result mainly from forward scatter. Forward scatter originates from particles of about wavelength size distributed throughout the lens. Most of the slit lamp image originates from small particle scatter (Rayleigh scatter). For a population of middle aged lenses it will be shown that both these scatter components remove around 10% of the light from the direct beam. For slit lamp observation close to the reflection angles, zones of discontinuity (Wasserspalten) at anterior and posterior parts of the lens show up as rough surface reflections. All these light scatter effects increase with age, but the correlations with age, and also between the different components, are weak. For retro-illumination imaging it will be argued that the density or opacity seen in areas of cortical or posterior subcapsular cataract show up because of light scattering, not because of light loss. NOTES: (1) Light scatter must not be confused with aberrations. Light penetrating the eye is divided into two parts: a relatively small part is scattered, and removed from the direct beam. Most of the light is not scattered, but continues as the direct beam. This non-scattered part is the basis for functional imaging, but its quality is under the control of aberrations. Aberrations deflect light mainly over small angles (light scatter is important because of the straylight effects over large angles (>1°), causing problems like glare and hazy vision. (2) The slit lamp image in older lenses and nuclear cataract is strongly influenced by absorption. However, this effect is greatly exaggerated by the light path lengths concerned. This

  18. Daytime Cognitive Performance in Response to Sunlight or Fluorescent Light Controlling for Sleep Duration

    Science.gov (United States)

    Ramos, Jhanic; Zamos, Adela; Rao, Rohit; Flynn-Evans, Erin

    2015-01-01

    Light is the primary synchronizer of the human circadian rhythm and also has acute alerting effects. Our study involves and comparing the alertness, performance and sleep of participants in the NASA Ames Sustainability Base, which uses sunlight as its primary light source, to in a traditional office building which uses overhead florescent lighting and varying exposure to natural light. The purpose of this study is to determine whether the use of natural lighting as a primary light source improves daytime cognitive function and promotes nighttime sleep. Participants from the Sustainability Base will be matched by gender and age to individuals working in other NASA buildings. In a prior study we found no differences in performance between those working in the Sustainability Base and those working in other buildings. Unexpectedly, we found that the average sleep duration among participants in both buildings was short, which likely obscured our ability to detect a difference the effect of light exposure on alertness. Given that such sleep deprivation has negative effects on cognitive performance, in this iteration of the study we are asking the participants to maintain a regular schedule with eight hours in bed each night in order to control for the effect of self-selected sleep restriction. Over the course of one week, we will ask the participants to wear actiwatches continuously, complete a psychomotor vigilance task (PVT) and digit symbol substitution task (DSST) three times per day, and keep daily sleepwork diaries. We hope that this study will provide data to support the idea that natural lighting and green architectural design are optimal to enhance healthy nighttime sleep patterns and daytime cognitive performance.

  19. APPLICATION OPTIMIZATION METHODS ARAS AND COPRAS IN THE CHOICE COMPACT FLUORESCENT LIGHT BULB

    Directory of Open Access Journals (Sweden)

    Ivana Zdravković

    2014-01-01

    Full Text Available Multicriteria methods are widely used for comparative evaluation of complicated technological and socio-economic processes, as well as to determine what is the best among a available options and ranking of alternatives based on their importance for a particular purpose. The methods of multi-criteria analysis can be improved decision-making in all sectors of the economy, because today, problems of decision solved on the basis of quantitative analysis. The paper discusses the election of fluorescent compact bulb using the method of multiple criteria decision, in this particular case method ARAS and method COPRAS.

  20. Retrofitting and the mu Problem

    Energy Technology Data Exchange (ETDEWEB)

    Green, Daniel; Weigand, Timo; /SLAC /Stanford U., Phys. Dept.

    2010-08-26

    One of the challenges of supersymmetry (SUSY) breaking and mediation is generating a {mu} term consistent with the requirements of electro-weak symmetry breaking. The most common approach to the problem is to generate the {mu} term through a SUSY breaking F-term. Often these models produce unacceptably large B{mu} terms as a result. We will present an alternate approach, where the {mu} term is generated directly by non-perturtative effects. The same non-perturbative effect will also retrofit the model of SUSY breaking in such a way that {mu} is at the same scale as masses of the Standard Model superpartners. Because the {mu} term is not directly generated by SUSY breaking effects, there is no associated B{mu} problem. These results are demonstrated in a toy model where a stringy instanton generates {mu}.

  1. Retrofit electrochromic glazing in a UK office

    Directory of Open Access Journals (Sweden)

    Ruth Kelly Waskett

    2014-12-01

    Full Text Available Electrochromic (EC glazing is now considered a viable alternative to fixed transmittance glazing. It has the potential to enable occupants to control daylight glare and solar heat gain without the use of blinds or external shading devices, giving users more access to daylight with all its inherent benefits. Furthermore, EC glazing can reduce energy consumption by decreasing cooling loads and electric lighting usage. Most research to date has studied the effects of EC glazing in scale models, computer simulations and full scale test rooms, and some of these studies have included human participants. However, there is a general lack of understanding regarding the performance and suitability of EC glazing in real-world working environments. A case study of the first UK retrofit application of EC glazing is being conducted in two adjacent offices in a university campus building. The offices are occupied by administration staff and have large southeastfacing windows. The existing double glazed units were replaced with commercially-available EC glazed units in 2012. Over a period of more than 18 months, the rooms were monitored intensively to record the effect of the EC glazing on both the physical room environment and the occupants themselves. A large amount of data from the monitoring programme is currently undergoing detailed analysis. Initial findings emerging from the installation and post-installation period are described in this paper.

  2. Triplet-triplet annihilation in highly efficient fluorescent organic light-emitting diodes: current state and future outlook.

    Science.gov (United States)

    Kondakov, Denis Y

    2015-06-28

    Studies of delayed electroluminescence in highly efficient fluorescent organic light-emitting diodes (OLEDs) of many dissimilar architectures indicate that the triplet-triplet annihilation (TTA) significantly increases yield of excited singlet states-emitting molecules in this type of device thereby contributes substantially to their efficiency. Towards the end of the 2000s, the essential role of TTA in realizing highly efficient fluorescent devices was widely recognized. Analysis of a diverse set of fluorescent OLEDs shows that high efficiencies are often cor-related to TTA extents. It is therefore likely that it is the long-term empirical optimization of OLED efficiencies that has resulted in fortuitous emergence of TTA as a large and ubiquitous contributor to efficiency. TTA contributions as high as 20-30% are common in the state-of-the-art OLEDs, and even become dominant in special cases, where TTA is shown to substantially exceed the spin-statistical limit. The fundamental features of OLED efficiency enhancement via TTA-molecular structure-dependent contributions, current density-dependent intensities in practical devices and frequently observed antagonistic relationships between TTA extent and OLED lifetime-came to be understood over the course of the next few years. More recently, however, there was much less reported progress with respect to all-important quantitative details of the TTA mechanism. It should be emphasized that, to this day and despite the decades of work on improving blue phosphorescent OLEDs as well as the recent advent of thermally activated delayed fluorescence OLEDs, the majority of practical blue OLEDs still rely on TTA. Considering such practical importance of fluorescent blue OLEDs, the design of blue OLED-compatible materials capable of substantially exceeding the spin-statistical limit in TTA, elimination of the antagonistic relationship between TTA-related efficiency gains and lifetime losses, and designing devices with an extended

  3. The influence of visible light and inorganic pigments on fluorescence excitation emission spectra of egg-, casein- and collagen-based painting media

    Science.gov (United States)

    Nevin, A.; Anglos, D.; Cather, S.; Burnstock, A.

    2008-07-01

    Spectrofluorimetric analysis of proteinaceous binding media is particularly promising because proteins employed in paintings are often fluorescent and media from different sources have significantly different fluorescence spectral profiles. Protein-based binding media derived from eggs, milk and animal tissue have been used for painting and for conservation, but their analysis using non-destructive techniques is complicated by interferences with pigments, their degradation and their low concentration. Changes in the fluorescence excitation emission spectra of films of binding media following artificial ageing to an equivalent of 50 and 100 years of museum lighting include the reduction of bands ascribed to tyrosine, tryptophan and Maillard reaction products and an increase in fluorescent photodegradation. Fluorescence of naturally aged paint is dependent on the nature of the pigment present and, with egg-based media, in comparison with un-pigmented films, emissions ascribed to amino acids are more pronounced.

  4. New light on ion channel imaging by total internal reflection fluorescence (TIRF) microscopy.

    Science.gov (United States)

    Yamamura, Hisao; Suzuki, Yoshiaki; Imaizumi, Yuji

    2015-05-01

    Ion channels play pivotal roles in a wide variety of cellular functions; therefore, their physiological characteristics, pharmacological responses, and molecular structures have been extensively investigated. However, the mobility of an ion channel itself in the cell membrane has not been examined in as much detail. A total internal reflection fluorescence (TIRF) microscope allows fluorophores to be imaged in a restricted region within an evanescent field of less than 200 nm from the interface of the coverslip and plasma membrane in living cells. Thus the TIRF microscope is useful for selectively visualizing the plasmalemmal surface and subplasmalemmal zone. In this review, we focused on a single-molecule analysis of the dynamic movement of ion channels in the plasma membrane using TIRF microscopy. We also described two single-molecule imaging techniques under TIRF microscopy: fluorescence resonance energy transfer (FRET) for the identification of molecules that interact with ion channels, and subunit counting for the determination of subunit stoichiometry in a functional channel. TIRF imaging can also be used to analyze spatiotemporal Ca(2+) events in the subplasmalemma. Single-molecule analyses of ion channels and localized Ca(2+) signals based on TIRF imaging provide beneficial pharmacological and physiological information concerning the functions of ion channels. Copyright © 2015 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

  5. Pulsed lasers versus continuous light sources in capillary electrophoresis and fluorescence detection studies: Photodegradation pathways and models

    Energy Technology Data Exchange (ETDEWEB)

    Boutonnet, Audrey; Morin, Arnaud [Picometrics Technologies, 478 Rue de la Découverte, Labège France (France); Petit, Pierre [Institut de Mathématiques, Université de Toulouse, Université Paul Sabatier, Toulouse CEDEX 04 (France); Vicendo, Patricia; Poinsot, Véréna [Laboratoire des IMRCP, UMR 5623, Université de Toulouse, Université Paul Sabatier, Toulouse CEDEX 04 (France); Couderc, François, E-mail: couderc@chimie.ups-tlse.fr [Laboratoire des IMRCP, UMR 5623, Université de Toulouse, Université Paul Sabatier, Toulouse CEDEX 04 (France)

    2016-03-17

    Pulsed lasers are widely used in capillary electrophoresis (CE) studies to provide laser induced fluorescence (LIF) detection. Unfortunately pulsed lasers do not give linear calibration curves over a wide range of concentrations. While this does not prevent their use in CE/LIF studies, the non-linear behavior must be understood. Using 7-hydroxycoumarin (7-HC) (10–5000 nM), Tamra (10–5000 nM) and tryptophan (1–200 μM) as dyes, we observe that continuous lasers and LEDs result in linear calibration curves, while pulsed lasers give polynomial ones. The effect is seen with both visible light (530 nm) and with UV light (355 nm, 266 nm). In this work we point out the formation of byproducts induced by pulsed laser upon irradiation of 7-HC. Their separation by CE using two Zeta LIF detectors clearly shows that this process is related to the first laser detection. All of these photodegradation products can be identified by an ESI-/MS investigation and correspond to at least two 7HC dimers. By using the photodegradation model proposed by Heywood and Farnsworth (2010) and by taking into account the 7-HC results and the fact that in our system we do not have a constant concentration of fluorophore, it is possible to propose a new photochemical model of fluorescence in LIF detection. The model, like the experiment, shows that it is difficult to obtain linear quantitation curves with pulsed lasers while UV-LEDs used in continuous mode have this advantage. They are a good alternative to UV pulsed lasers. An application involving the separation and linear quantification of oligosaccharides labeled with 2-aminobezoic acid is presented using HILIC and LED (365 nm) induced fluorescence. - Highlights: • No linear calibration curves are obtained in CE/Pulsed-LIF detection. • Photodegradation and photodimerisation are responsible of this non linearity. • A mathematical model of this phenomenon is presented. • 7 hydroxycoumarin in CE/LIF is used to verify the

  6. Accelerating the energy retrofit of commercial buildings using a database of energy efficiency performance

    International Nuclear Information System (INIS)

    Lee, Sang Hoon; Hong, Tianzhen; Piette, Mary Ann; Sawaya, Geof; Chen, Yixing; Taylor-Lange, Sarah C.

    2015-01-01

    Small and medium-sized commercial buildings can be retrofitted to significantly reduce their energy use, however it is a huge challenge as owners usually lack of the expertise and resources to conduct detailed on-site energy audit to identify and evaluate cost-effective energy technologies. This study presents a DEEP (database of energy efficiency performance) that provides a direct resource for quick retrofit analysis of commercial buildings. DEEP, compiled from the results of about ten million EnergyPlus simulations, enables an easy screening of ECMs (energy conservation measures) and retrofit analysis. The simulations utilize prototype models representative of small and mid-size offices and retails in California climates. In the formulation of DEEP, large scale EnergyPlus simulations were conducted on high performance computing clusters to evaluate hundreds of individual and packaged ECMs covering envelope, lighting, heating, ventilation, air-conditioning, plug-loads, and service hot water. The architecture and simulation environment to create DEEP is flexible and can expand to cover additional building types, additional climates, and new ECMs. In this study DEEP is integrated into a web-based retrofit toolkit, the Commercial Building Energy Saver, which provides a platform for energy retrofit decision making by querying DEEP and unearthing recommended ECMs, their estimated energy savings and financial payback. - Highlights: • A DEEP (database of energy efficiency performance) supports building retrofit. • DEEP is an SQL database with pre-simulated results from 10 million EnergyPlus runs. • DEEP covers 7 building types, 6 vintages, 16 climates, and 100 energy measures. • DEEP accelerates retrofit of small commercial buildings to save energy use and cost. • DEEP can be expanded and integrated with third-party energy software tools.

  7. Seismic retrofit guidelines for Utah highway bridges.

    Science.gov (United States)

    2009-05-01

    Much of Utahs population dwells in a seismically active region, and many of the bridges connecting transportation lifelines predate the rigorous seismic design standards that have been developed in the past 10-20 years. Seismic retrofitting method...

  8. Quantifying the Financial Benefits of Multifamily Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Philbrick, D. [The Partnership for Advanced Residential Retrofit, Chicago, IL (United States); Scheu, R. [The Partnership for Advanced Residential Retrofit, Chicago, IL (United States); Brand, L. [The Partnership for Advanced Residential Retrofit, Chicago, IL (United States)

    2016-01-29

    Increasing the adoption of energy efficient building practices will require the energy sector to increase their understanding of the way that retrofits affect multifamily financial performance as well as how those indicators are interpreted by the lending and appraisal industries. This project analyzed building, energy, and financial program data as well as other public and private data to examine the relationship between energy efficiency retrofits and financial performance on three levels: building, city, and community. The project goals were to increase the data and analysis in the growing body of multifamily financial benefits work as well provide a framework for other geographies to produce similar characterization. The goals are accomplished through three tasks. Task one: A pre- and post-retrofit analysis of thirteen Chicago multifamily buildings. Task two: A comparison of Chicago income and expenses to two national datasets. Task three: An in-depth look at multifamily market sales data and the subsequent impact of buildings that undergo retrofits.

  9. An assessment of the importance ofexposure routes to the uptake and internal localisation of fluorescent nanoparticles in zebrafish (Danio rerio), using light sheet microscopy

    DEFF Research Database (Denmark)

    Skjolding, Lars Michael; Ašmonaitė, G; Jølck, Rasmus Irming

    2017-01-01

    A major challenge in nanoecotoxicology is finding suitable methods to determine the uptake and localisation of nanoparticles on a whole-organism level. Some uptake methods have been associated with artefacts induced by sample preparation, including staining for electron microscopy. This study used...... light sheet microscopy (LSM) to define the uptake and localisation of fluorescently labelled nanoparticles in living organisms with minimal sample preparation. Zebrafish (Danio rerio) were exposed to fluorescent gold nanoparticles (Au NPs) and fluorescent polystyrene NPs via aqueous or dietary exposure...

  10. Application of exciplex in the fabrication of white organic light emitting devices with mixed fluorescent and phosphorescent layers

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Dan; Duan, Yahui; Yang, Yongqiang [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun 130012 (China); Hu, Nan [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun 130012 (China); Changchun University of Science and Technology, Changchun 130012 (China); Wang, Xiao [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun 130012 (China); Sun, Fengbo [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun 130012 (China); Changchun University of Science and Technology, Changchun 130012 (China); Duan, Yu, E-mail: duanyu@jlu.edu.cn [State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun 130012 (China)

    2015-10-15

    In this study, a highly efficient fluorescent/phosphorescent white organic light-emitting device (WOLED) was fabricated using exciplex light emission. The hole-transport material 4,4',4''-tris(N-carbazolyl)triphenylamine (TCTA), and electron-transport material, 4,7-diphenyl-1,10-phenanthroline (Bphen), were mixed to afford a blue-emitting exciplex. The WOLED was fabricated with a yellow phosphorescent dye, Ir(III) bis(4-phenylthieno [3,2-c] pyridinato-N,C{sup 2'}) acetylacetonate (PO-01), combined with the exciplex. In this structure, the energy can be efficiently transferred from the blend layer to the yellow phosphorescent dye, thus improving the efficiency of the utilization of the triplet exciton. The maximum power efficiency of the WOLED reached a value 9.03 lm/W with an external quantum efficiency of 4.3%. The Commission Internationale de I'Eclairage (CIE) color coordinates (x,y) of the device were from (0.39, 0.45) to (0.27, 0.31), with a voltage range of 4–9 V. - Highlights: • An exciplex/phosphorescence hybrid white OLED was fabricated for the first time with blue/orange complementary emitters. • By using exciplex as the blue emitter, non-radiative triplet-states on the exciplex can be harvested for light-emission by transferring them to low triplet-state phosphors.

  11. Design and testing of a separate-type lighting system using solar energy and cold-cathode fluorescent lamps

    Energy Technology Data Exchange (ETDEWEB)

    Yang, J.-P. [Department of Electrical Engineering, China Institute of Technology, Taipei, Taiwan 115, Taiwan (China)]. E-mail: april4120@tp.edu.tw; Hsiao, H.-C. [Department of Electrical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan 106, Taiwan (China)]. E-mail: hsiao@mouse.ee.ntust.edu.tw

    2007-01-15

    This paper presents a solar-powered lighting system, using cold-cathode fluorescent-lamps (CCFLs), with its battery-charging circuit and lamp-ignition circuit being separated so that its solar panels can be installed at any distance deemed necessary away from the lighting site in order to receive the maximum solar energy available. This system adopts the maximum-power point tracking (MPPT) method to control the power output of the solar panels and uses the zero-voltage switching (ZVS) DC-DC converter, as the charging circuit, to increase the panels' power generation efficiency and the charging circuit's conversion efficiency. The electronic ballast circuit for the CCFL is constructed with a half-bridge inverter, a resonant inductor, and a Rosen-type piezoelectric transformer, which forms a piezoelectric resonant-type inverter: to simplify the circuitry and to improve the power conversion efficiency, the ballast circuit is designed to directly step up the battery voltage in igniting the lamp. We also establish the transmission-parameter model for the piezoelectric resonant-type inverter to provide the base for the electric-power circuit design. Our experimental results indicate that the proposed system possesses some advantages, such as greater energy efficiency, circuitry simplicity, and so on, and is suitable for night lighting in house yards, parks and advertising panels.

  12. Design and testing of a separate-type lighting system using solar energy and cold-cathode fluorescent lamps

    International Nuclear Information System (INIS)

    Yang, J.-P.; Hsiao, H.-C.

    2007-01-01

    This paper presents a solar-powered lighting system, using cold-cathode fluorescent-lamps (CCFLs), with its battery-charging circuit and lamp-ignition circuit being separated so that its solar panels can be installed at any distance deemed necessary away from the lighting site in order to receive the maximum solar energy available. This system adopts the maximum-power point tracking (MPPT) method to control the power output of the solar panels and uses the zero-voltage switching (ZVS) DC-DC converter, as the charging circuit, to increase the panels' power generation efficiency and the charging circuit's conversion efficiency. The electronic ballast circuit for the CCFL is constructed with a half-bridge inverter, a resonant inductor, and a Rosen-type piezoelectric transformer, which forms a piezoelectric resonant-type inverter: to simplify the circuitry and to improve the power conversion efficiency, the ballast circuit is designed to directly step up the battery voltage in igniting the lamp. We also establish the transmission-parameter model for the piezoelectric resonant-type inverter to provide the base for the electric-power circuit design. Our experimental results indicate that the proposed system possesses some advantages, such as greater energy efficiency, circuitry simplicity, and so on, and is suitable for night lighting in house yards, parks and advertising panels

  13. CALiPER Exploratory Study: Recessed Troffer Lighting

    Energy Technology Data Exchange (ETDEWEB)

    Miller, Naomi J.; Royer, Michael P.; Poplawski, Michael E.

    2013-04-28

    This report describes an exploration of troffer lighting as used in office and classroom spaces, which was conducted by the CALiPER program. Twenty-four pairs of 2×2 and 2×4 troffers were procured anonymously, documented, tested for photometric and electrical performance, and installed in a mockup office space in Portland, Oregon. Three of the pairs were T8 fluorescent benchmark products, 12 were dedicated LED troffers, five were fluorescent troffers modified for LED lamps (sometimes referred to as "tubes"), and another four troffers were modified with LED retrofit kits. The modifications were performed by a commercial electrical contractor, following the instructions provided by the retrofit lamp or kit manufacturer. Once installed in the mockup facility, the converted luminaires were examined by a NRTL (Nationally Recognized Testing Laboratory) safety expert, who provided feedback on safety issues. In September 2012, a group of lighting designers, engineers, and facility managers were brought in to observe the LED luminaires in comparison to fluorescent benchmarks. This report documents performance in measures that go beyond illuminance values or luminaire efficacy. Dedicated LED troffers are ready to compete with fluorescent troffers in terms of efficacy (lumens per watt), and in many lighting quality issues such as glare, light distribution, visual appearance, and color quality. That is not to say that each one is stellar, but each one tested in this CALiPER study bested the fluorescent benchmarks in terms of efficacy, and almost all were rated highly in several categories -- only one luminaire of twelve performed consistently poorly.

  14. Analysis of complex samples using a portable multi-wavelength light emitting diode (LED) fluorescence spectrometer

    Science.gov (United States)

    Spectroscopic analysis of chemically complex samples often requires an increase n the dimensionality of the measured response surface. This often involves the measurement of emitted light intensities as functions of both wavelengths of excitation and emission resulting in the generation of an excita...

  15. Screening of Aflatoxin-producing mould isolates based on fluorescence production on a specific medium under ultraviolet light

    Directory of Open Access Journals (Sweden)

    Razzaghi Abyaneh M

    2000-09-01

    Full Text Available Aflatoxin contamination of food and feedstuffs is a serious economic and health hazard. Over a period of two years, 35 samples of raw pistachio nuts collected from different parts of Iran were analysed for the presence of aflatoxigenic strains of the Aspergillus flavus group. Eighty-five isolates of A.flavus and A.parasiticuswere identified based on specific yello-orange pigment production in the reverse of the colonies on Aspergillus flavus and parasiticus agar (AFPA medium and microscopic criteria. These isolates were present in 65.71% of the samples and their aflatoxin-producting ability was examined. At first , 12 out ot the 15 aflatoxin-producing isolates were selected based on bright blue fluorescence colour on aflatoxin-producing ability (APA medium. Later, all of the isolates were identifid after culturing on sucrose-low salts (SLS medium. Furthermore, aflatoxin production on APA and SLS media was confirmed when the mycelia and media were extracte and analysed fo aflatoxins on TLC plates. A.flavus strions produced mainly aflatoxin B1 with concentrations rangiong from 0.21 to 1.49 µg/g of APA medium, where as A.parasiticus strain produced all four aflatoxins (B1, B2, G1 and G2 with 3.71 µg aflatoxin B1/g of the medium. Isolates other than A.flavus and A.parasiticus grown on this specific culture medium failed to produce a characteristic fluorescence under UV light. Evidences from this study indicate that fluorescent production on APA medium is a reliable screening test for distinguishing potential aflatoxin-producing strains of A.parasiticus from nontoxigenic strains.

  16. Hybrid white organic light-emitting devices based on phosphorescent iridium–benzotriazole orange–red and fluorescent blue emitters

    International Nuclear Information System (INIS)

    Xia, Zhen-Yuan; Su, Jian-Hua; Chang, Chi-Sheng; Chen, Chin H.

    2013-01-01

    We demonstrate that high color purity or efficiency hybrid white organic light-emitting devices (OLEDs) can be generated by integrating a phosphorescent orange–red emitter, bis[4-(2H-benzotriazol-2-yl)-N,N-diphenyl-aniline-N 1 ,C 3 ] iridium acetylacetonate, Ir(TBT) 2 (acac) with fluorescent blue emitters in two different emissive layers. The device based on deep blue fluorescent material diphenyl-[4-(2-[1,1′;4′,1″]terphenyl-4-yl-vinyl)-phenyl]-amine BpSAB and Ir(TBT) 2 (acac) shows pure white color with the Commission Internationale de L'Eclairage (CIE) coordinates of (0.33,0.30). When using sky-blue fluorescent dopant N,N′-(4,4′-(1E,1′E)-2,2′-(1,4-phenylene)bis(ethene-2,1-diyl) bis(4,1-phenylene))bis(2-ethyl-6-methyl-N-phenylaniline) (BUBD-1) and orange–red phosphor with a color-tuning phosphorescent material fac-tris(2-phenylpyridine) iridium (Ir(ppy) 3 ), it exhibits peak luminance yield and power efficiency of 17.4 cd/A and 10.7 lm/W, respectively with yellow-white color and CIE color rendering index (CRI) value of 73. - Highlights: ► An iridium-based orange–red phosphor Ir(TBT) 2 (acac) was applied in hybrid white OLEDs. ► Duel- and tri-emitter WOLEDs were achieved with either high color purity or efficiency performance. ► Peak luminance yield of tri-emitter WOLEDs was 17.4 cd/A with yellow-white color and color rendering index (CRI) value of 73.

  17. Hybrid white organic light-emitting devices based on phosphorescent iridium-benzotriazole orange-red and fluorescent blue emitters

    Energy Technology Data Exchange (ETDEWEB)

    Xia, Zhen-Yuan, E-mail: xiazhenyuan@hotmail.com [Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (China); Su, Jian-Hua [Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, Shanghai 200237 (China); Chang, Chi-Sheng; Chen, Chin H. [Display Institute, Microelectronics and Information Systems Research Center, National Chiao Tung University, Hsinchu, Taiwan 300 (China)

    2013-03-15

    We demonstrate that high color purity or efficiency hybrid white organic light-emitting devices (OLEDs) can be generated by integrating a phosphorescent orange-red emitter, bis[4-(2H-benzotriazol-2-yl)-N,N-diphenyl-aniline-N{sup 1},C{sup 3}] iridium acetylacetonate, Ir(TBT){sub 2}(acac) with fluorescent blue emitters in two different emissive layers. The device based on deep blue fluorescent material diphenyl-[4-(2-[1,1 Prime ;4 Prime ,1 Double-Prime ]terphenyl-4-yl-vinyl)-phenyl]-amine BpSAB and Ir(TBT){sub 2}(acac) shows pure white color with the Commission Internationale de L'Eclairage (CIE) coordinates of (0.33,0.30). When using sky-blue fluorescent dopant N,N Prime -(4,4 Prime -(1E,1 Prime E)-2,2 Prime -(1,4-phenylene)bis(ethene-2,1-diyl) bis(4,1-phenylene))bis(2-ethyl-6-methyl-N-phenylaniline) (BUBD-1) and orange-red phosphor with a color-tuning phosphorescent material fac-tris(2-phenylpyridine) iridium (Ir(ppy){sub 3} ), it exhibits peak luminance yield and power efficiency of 17.4 cd/A and 10.7 lm/W, respectively with yellow-white color and CIE color rendering index (CRI) value of 73. - Highlights: Black-Right-Pointing-Pointer An iridium-based orange-red phosphor Ir(TBT){sub 2}(acac) was applied in hybrid white OLEDs. Black-Right-Pointing-Pointer Duel- and tri-emitter WOLEDs were achieved with either high color purity or efficiency performance. Black-Right-Pointing-Pointer Peak luminance yield of tri-emitter WOLEDs was 17.4 cd/A with yellow-white color and color rendering index (CRI) value of 73.

  18. Fluorescent lighting system of modified fast starting system; Sistema de alumbrado fluorescente de arranque rapido modificado

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza E, Ernesto J. [Manufacturera De Reactores, S. A., Mexico, D. F. (Mexico)

    1993-12-31

    This paper puts into perspective the possible energy saving in using hybrid ballasts for the operation of fast starting lamps. It presents in summarized form some fundamentals of the fluorescent lamps operation, as well as the fast starting modified systems. Also the results of field operation are shown, as well as the effects on the useful life of the lamp T12 as well as the T8. [Espanol] Este trabajo pone en perspectiva el ahorro de energia posible, al emplear balastros hibridos para operar lamparas de arranque rapido. Presenta en forma resumida algunos fundamentos de la operacion de lamparas fluorescentes, asi como de los sistemas de arranque rapido modificado. Se muestran tambien resultados de operacion de campo, asi como los efectos sobre la vida util de las lamparas, tanto T12 como T8.

  19. Optimized Application of MSR and Steam Turbine Retrofits in Nuclear Power Plants

    International Nuclear Information System (INIS)

    Crossland, Robert; McCoach, John; Gagelin, Jean-Philippe

    2004-01-01

    The benefit to a nuclear power plant from a steam turbine retrofit has often been clearly demonstrated in recent years but, for light water nuclear plants, the Moisture Separator Reheaters (MSRs) are also of prime importance. This paper describes how refurbishment of these crucial components can only provide full potential performance benefit when made in conjunction with a steam turbine retrofit (although in practice these activities are frequently separated). Examples are given to show how combined application is best handled within a single organization to ensure optimized integration into the thermal cycle. (authors)

  20. Fertility potential in a man with ankylosing spondylitis as revealed by semen analysis by light, electron and fluorescence microscopy.

    Science.gov (United States)

    Chatzimeletiou, Katerina; Galanis, Nikiforos; Karagiannidis, Alexandros; Sioga, Antonia; Pados, George; Goulis, Dimitrios; Kalpatsanidis, Antonis; Tarlatzis, Basil C

    2018-01-01

    Ankylosing spondylitis affects 0.1%-0.5% of the adult population. The aim was to investigate the possible effects of both the disease and its treatment on semen quality by performing a highly detailed analysis in a man with ankylosing spondylitis, presenting for infertility. Sperm characteristics were evaluated by light microscopy, morphology by electron microscopy (transmission electron microscopy), DNA fragmentation by terminal deoxynucleotidyl transferase dUTP nick end labeling using fluorescence microscopy and chromosomal abnormalities by fluorescence in situ hybridisation using probes for chromosomes 13,15,16,18,21,22,X and Y. There was no evidence for an effect of either ankylosing spondylitis or its treatment with celecoxib and sulphasalazine on sperm quality as all parameters including concentration, motility, DNA fragmentation and aneuploidy incidence were within normal limits. Transmission electron microscopy, however, revealed a high incidence of head, neck and tail abnormalities, as well as the presence of immature sperm and phagocytes. Hysteroscopic removal of an endometrial polyp enabled the achievement of a spontaneous pregnancy and the delivery of a healthy boy.

  1. A light-up fluorescent probe for citrate detection based on bispyridinum amides with aggregation-induced emission feature.

    Science.gov (United States)

    Liu, Chenchen; Hang, Yandi; Jiang, Tao; Yang, Ji; Zhang, Xiao; Hua, Jianli

    2018-02-01

    Citrate is an important intermediate in the citric acid cycle, a vital metabolic pathway for animals, plants and bacteria. It is of great significance to detect its levels in human beings because several diseases may cause the abnormal of citrate. In this paper, a new turn-on fluorescent sensor (TPE-Py) using the classic tetraphenylethylene (TPE) as the aggregation-induced emission (AIE) fluorophore and bipyridinium-based amides as the recognition receptor has been synthesized for the detection of citrate. The probe exhibits good selectivity and sensitivity to citrate with a relatively low detection limit (1.0 × 10 -7 M). The enhancement of the fluorescence is relevant with the AIE property based on the complexation of TPE-Py with citrate caused by the hydrogen bonding and electrostatic interactions between the bipyridinium diamides and citrate, which has been proved by 1 H NMR and mass spectra titration, scanning electronic microscope and dynamic light scattering analyses. More importantly, the quantification of citrate in artificial urine may develop TPE-Py fluorometric probe for the citrate detection in real biosystems. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Uptake and localization of fluorescent labelled gold nanoparticles in living zebrafish (Danio rerio) using Light Sheet Microscopy

    DEFF Research Database (Denmark)

    Skjolding, Lars Michael; Asmonaite, G.; Jolk, R.

    2015-01-01

    Despite nanoparticles being used in many different products and applications, the effects and fate in the environment are still not well understood. Uptake of nanoparticles into cells has been shown in vitro and in vivo. However, it is challenging to find suitable methods to identify uptake...... and determine localization on a whole organism level. Furthermore, methods used to identify nanoparticle uptake have been associated with artefacts induced by sample preparation including staining methods for electron microscopy.  This study used Fluorescent Light Sheet Microscopy (FLSM) to determine uptake...... to the particles through the diet or the water phase in a series of separate experiments. In the dietary exposure experiments Artemia salina were exposed to 1 mg Au/L for 24h before being fed to D. rerio. For exposure through the water phase 1 mg Au/L was added directly to aquaria holding the fish and non...

  3. Fluorescent optical position sensor

    Science.gov (United States)

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  4. The Role of Light-Induced Fluorescence in the Treatment of Smooth Surface Carious Lesions with Icon Infiltration and the Results After 1 Year

    OpenAIRE

    Kabaktchieva R.; Gateva N.; Peycheva K.

    2014-01-01

    Caries infiltration is a novel technique that brings out immediate esthetic improvement in the opacity of the white spot lesions. Light-induced fluorescence method is a modern caries diagnostic method. In this study SoproLife camera (Acteon, France) was applied for diagnosing and follow-up of the results. The aims of this in vivo study are to test the role of light-induced fluorescence method (SoploLife camera) in the diagnosis of non-cavitated smooth surfaces carious lesions (ICDAS codes 1 a...

  5. ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy.

    Science.gov (United States)

    Brama, Elisabeth; Peddie, Christopher J; Wilkes, Gary; Gu, Yan; Collinson, Lucy M; Jones, Martin L

    2016-12-13

    In-resin fluorescence (IRF) protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution correlation of fluorophore to organelle. IRF samples also offer a unique opportunity to automate correlative imaging workflows. Here we present two new locator tools for finding and following fluorescent cells in IRF blocks, enabling future automation of correlative imaging. The ultraLM is a fluorescence microscope that integrates with an ultramicrotome, which enables 'smart collection' of ultrathin sections containing fluorescent cells or tissues for subsequent transmission electron microscopy or array tomography. The miniLM is a fluorescence microscope that integrates with serial block face scanning electron microscopes, which enables 'smart tracking' of fluorescent structures during automated serial electron image acquisition from large cell and tissue volumes.

  6. Fluorescence enhancement and nonreciprocal transmission of light waves by nanomaterial interfaces

    Science.gov (United States)

    Nyman, M.; Shevchenko, A.; Kaivola, M.

    2017-11-01

    In an optically absorbing or amplifying linear medium, the energy flow density of interfering optical waves is in general periodically modulated in space. This makes the wave transmission through a material boundary, as described by the Fresnel transmission coefficients, nonreciprocal and apparently violating the energy conservation law. The modulation has been previously described in connection to ordinary homogeneous nonmagnetic materials. In this work, we extend the description to nanomaterials with designed structural units that can be magnetic at optical frequencies. We find that in such a "metamaterial" the modulation in energy flow can be used to enhance optical far-field emission in spite of the fact that the material is highly absorbing. We also demonstrate a nanomaterial design that absorbs light, but simultaneously eliminates the power flow modulation and returns the reciprocity, which is impossible to achieve with a nonmagnetic material. We anticipate that these unusual optical effects can be used to increase the efficiency of nanostructured light emitters and absorbers, such as light-emitting diodes and solar cells.

  7. Fluorescent minerals - A potential source of UV protection and visible light for the growth of green algae and cyanobacteria in extreme cosmic environments

    Science.gov (United States)

    Omairi, Tareq; Wainwright, Milton

    2015-07-01

    We propose that green algae (Chlorella variabilis and Dunaliella tertiolecta) and cyanobacteria (Synechococcus elongatus and Nostoc commune) can grow inside fluorescent rock minerals which convert damaging UV light to visible light, thereby allowing these organisms to survive and thrive in UV-rich environments without (or with limited) visible light, which would otherwise be inimical to them. The four microorganisms were incubated inside fluorescent rocks composed of fluorite, calcite and pyrite. The resultant growth was then measured following exposure to UV radiation, with the use of optical density and measurement of chlorophyll concentration. Results show that the microorganisms were shielded from harmful UV in these semi-transparent rocks, while at the same time benefiting from the fact that the minerals converted UV to visible light; this have been shown by a statistically significant increase in their growth, which although lower than when the cells were incubated in sunlight, was significantly higher than in controls incubated in the dark.

  8. Bactericidal Activity of Aqueous Acrylic Paint Dispersion for Wooden Substrates Based on TiO₂ Nanoparticles Activated by Fluorescent Light.

    Science.gov (United States)

    Zuccheri, Tommaso; Colonna, Martino; Stefanini, Ilaria; Santini, Cecilia; Gioia, Diana Di

    2013-08-02

    The photocatalytic effect of TiO 2 has great potential for the disinfection of surfaces. Most studies reported in the literature use UV activation of TiO₂, while visible light has been used only in a few applications. In these studies, high concentrations of TiO₂, which can compromise surface properties, have been used. In this work, we have developed an acrylic-water paint dispersion containing low TiO₂ content (2 vol %) for the inactivation of microorganisms involved in hospital-acquired infections. The nanoparticles and the coating have been characterized using spectroscopic techniques and transmission electron microscopy, showing their homogenous dispersion in the acrylic urethane coating. A common fluorescent light source was used to activate the photocatalytic activity of TiO₂. The paint dispersion showed antimicrobial activity against Escherichia coli , Staphylococcus aureus and Pseudomonas aeruginosa . The coating containing the TiO₂ nanoparticles maintained good UV stability, strong adhesion to the substrate and high hardness. Therefore, the approach used is feasible for paint formulation aimed at disinfection of healthcare surfaces.

  9. Color-tunable and stable-efficiency white organic light-emitting diode fabricated with fluorescent-phosphorescent emission layers

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Su-Hua, E-mail: shya@cc.kuas.edu.tw; Shih, Po-Jen; Wu, Wen-Jie; Huang, Yi-Hua

    2013-10-15

    White organic light emitting diodes (OLEDs) were fabricated for color-tunable lighting applications. Fluorescent and phosphorescent hybrid emission layers (EMLs) were used to enhance the luminance and stability of the devices, which have blue-EML/CBP interlayer/green-EML/phosphorescent-sensitized-EML/red-EML structures. The influence of the composition and structure of the EMLs on the electroluminescence properties of the devices were investigated from the viewpoint of their emission spectra. The possible exciton harvesting, diffusion, transport, and annihilation processes occurring in the EMLs were also evaluated. A maximum luminance intensity of 7400 cd/m{sup 2} and a highly stable current efficiency of 3.2 cd/A were obtained. Good color tunability was achieved for the white OLEDs; the chromatic coordinates linearly shifted from pure white (0.300, 0.398) to cold white (0.261, 0.367) when the applied voltage was varied from 10 to 14 V. -- Highlights: • Exciton harvesting, diffusion, transport, and annihilation processes were evaluated. • The electroluminescence properties were investigated from the viewpoint of the emission spectra. • Good color tunability and stable-efficiency were achieved for the white OLEDs.

  10. Highly efficient exciplex organic light-emitting diodes using thermally activated delayed fluorescent emitters as donor and acceptor materials

    Science.gov (United States)

    Jeon, Sang Kyu; Yook, Kyoung Soo; Lee, Jun Yeob

    2016-06-01

    Highly efficient exciplex type organic light-emitting diodes were developed using thermally activated delayed fluorescent emitters as donors and acceptors of an exciplex. Blue emitting bis[4-(9,9-dimethyl-9,10-dihydroacridine)phenyl]sulfone (DMAC-DPS) was a donor and 9,9‧-(5-(4,6-diphenyl-1,3,5-triazin-2-yl)-1,3-phenylene)bis(9H-carbazole) (DDCzTrz) and 9,9‧,9″-(5-(4,6-diphenyl-1,3,5-triazin-2-yl)benzene-1,2,3-triyl)tris(9H-carbazole) (TCzTrz) were acceptor materials. The exciplexes of DMAC-DPS:TCzTrz and DMAC-DPS:DDCzTrz resulted in high photoluminescence quantum yield and high quantum efficiency in the green exciplex organic light-emitting diodes. High quantum efficiencies of 13.4% and 15.3% were obtained in the DMAC-DPS:DDCzTrz and DMAC-DPS:TCzTrz exciplex devices.

  11. Chlorophyll fluorescence ratio F685/F735 in brown algae and its variation under excitation by two types of light and dehydration

    Science.gov (United States)

    Wu, Baogan; Zang, Rubo

    1993-03-01

    Calculation and comparative study of the chlorophyll fluorescence ratio F685/F735 in brown alage ( Laminaria japonica, Underia pinnatifida and Padina crassa) excited by blue and green light showed that the fluorescence ratios were higher when the algae were excited by blue light (440 nm), but reduced obviously under green light (540 nm) excitation. The values also reduced under dehydration but could recover during rehydration if the stress was not serious. The variation of the fluorescence ratio under dehydration was mainly because changes in fluorescence emission at 735 nm were always sharper than those at 685 nm. The ratio was sensitive to stress and has potential as a stress indicator in phycological research. Measurement of the fluorescence excitation spectra showed that the only peak at 540 nm changed apparently during dehydration. It meant that the function of the Ch1 a/Fucoxanthin protein complex for energy transfer was easily inhibited by water stress. However, no variation of the ratio was found in green alga Ulva pertusa under dehydration. So it seemed to be something special for brown algae. The mechanism of this phenomenon is still unclear but may be related to the photosynthetic pigments and structural characteristics of brown algae.

  12. Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System

    Science.gov (United States)

    Loeffler, Juergen; Henke, Norbert; Hebart, Holger; Schmidt, Diethard; Hagmeyer, Lars; Schumacher, Ulrike; Einsele, Hermann

    2000-01-01

    The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The sensitivity of the assay was comparable to those of previously described PCR protocols (5 CFU/ml). Specific detection of C. albicans and A. fumigatus could be achieved. The assay showed a high reproducibility of 96 to 99%. The assay was linear in a range between 101 and 104 Aspergillus conidia. As capillaries do not have to be reopened for post-PCR analysis, the risk of carryover contaminations could be minimized. The Light Cycler allowed quantification of the fungal loads in a limited number of clinical specimens from patients with hematological malignancies and histologically proven invasive fungal infections. Five of nine positive samples had fungal loads between 5 and 10 CFU/ml of blood, two of nine positive samples had fungal loads between 10 and 100 CFU/ml of blood, and two of nine samples had fungal loads of more than 100 CFU/ml of blood. All samples were also found to be PCR positive by PCR–enzyme-linked immunosorbent assay analysis. PMID:10655350

  13. Carbon Absorber Retrofit Equipment (CARE)

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Eric [Neumann Systems Group, Incorporated, Colorado Springs, CO (United States)

    2015-12-23

    During Project DE-FE0007528, CARE (Carbon Absorber Retrofit Equipment), Neumann Systems Group (NSG) designed, installed and tested a 0.5MW NeuStream® carbon dioxide (CO2) capture system using the patented NeuStream® absorber equipment and concentrated (6 molal) piperazine (PZ) as the solvent at Colorado Springs Utilities’ (CSU’s) Martin Drake pulverized coal (PC) power plant. The 36 month project included design, build and test phases. The 0.5MW NeuStream® CO2 capture system was successfully tested on flue gas from both coal and natural gas combustion sources and was shown to meet project objectives. Ninety percent CO2 removal was achieved with greater than 95% CO2product purity. The absorbers tested support a 90% reduction in absorber volume compared to packed towers and with an absorber parasitic power of less than 1% when configured for operation with a 550MW coal plant. The preliminary techno-economic analysis (TEA) performed by the Energy and Environmental Research Center (EERC) predicted an over-the-fence cost of $25.73/tonne of CO2 captured from a sub-critical PC plant.

  14. Ultraviolet radiation levels associated with the use of fluorescent general lighting, UV-A and UV-B lamps in the workplace and home

    CERN Document Server

    Whillock, M; MacKinlay, Alistair F; Mundy, S J; Todd, Carl David

    1988-01-01

    A detailed programme of measurements was undertaken by NRPB to determine the ultraviolet irradiance levels likely to be encountered in the workplace and in the home, where fluorescent lighting is used. Assessments have been made of the possible potential risk of the induction of acute effects (photokeratitis, erythema) and of inducing malignant melanoma and non-malignant melanoma skin cancers resulting from exposure to commonly used fluorescent lamps. The optical absorption properties of materials commonly used in diffusers and controllers in commercial and domestic lighting units were also measured. Irradiance data, both weighted (for biological effectiveness) and unweighted, for various lamp types are presented in the report, together with some typical spectral output distributions. The results show that at commonly used illumination levels the UVR emissions from general and special fluorescent lamps presented neither an acute nor a significant chronic hazard. High UV-B emission levels were measured from 'U...

  15. Enhancement and Quenching of Fluorescence by Silver Nanoparticles in Organic Light-Emitting Diodes

    Directory of Open Access Journals (Sweden)

    Ying-Chung Chen

    2013-01-01

    Full Text Available The influence of silver nanoparticles (SNPs on the performance of organic light-emitting diodes (OLEDs is investigated in this study. The SNPs are introduced between the electron-transport layers by means of thermal evaporation. SNPs are found to have the surface plasmon resonance at wavelength 525 nm when the mean particle size of SNPs is 34 nm. The optimized OLED, in terms of the spacing between the emitting layer and SNPs, is found to have the maximum luminance 2.4 times higher than that in the OLED without SNPs. The energy transfer between exciton and surface plasmons with the different spacing distances has been studied.

  16. White light emission from fluorescent SiC with porous surface

    DEFF Research Database (Denmark)

    Lu, Weifang; Ou, Yiyu; Fiordaliso, Elisabetta Maria

    2017-01-01

    the bulk fuorescent SiC layer. A high color rendering index of 81.1 has been achieved. Photoluminescence spectra in porous layers fabricated in both commercial n-type and lab grown N-B co-doped 6H-SiC show two emission peaks centered approximately at 460nm and 530nm. Such bluegreen emission phenomenon can......, the photoluminescence intensity from the porous layer was signifcant enhanced by a factor of more than 12. Using a porous layer of moderate thickness (~10µm), high-quality white light emission was realized by combining the independent emissions of blue-green emission from the porous layer and yellow emission from...

  17. The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth

    NARCIS (Netherlands)

    Adeyemi, A.A.; Jarad, F.D.; de Josselin de Jong, E.; Pender, N.; Higham, S.M.

    2010-01-01

    This study reports the development and evaluation of a novel method using quantitative light-induced fluorescence (QLF), which enables its use for quantifying and assessing whole tooth surface staining and tooth whitening. The method was compared with a spectrophotometer to assess reliability. Two

  18. Choosing the Right Fireplace or Fireplace Retrofit Device

    Science.gov (United States)

    This page is about choosing a fireplace or fireplace retrofit device, including information on hang tags and a list of fireplaces and retrofits that have qualified under the voluntary fireplace program

  19. Green light-emitting polyepinephrine-based fluorescent organic dots and its application in intracellular metal ions sensing.

    Science.gov (United States)

    Gao, Zhong Feng; Li, Ting Ting; Xu, Xiao Lei; Liu, Yi Yao; Luo, Hong Qun; Li, Nian Bing

    2016-09-15

    In this paper, we present a class of bio-dots, polyepinephrine (PEP)-based fluorescent organic dots (PEP-FODs) for selective and sensitive detection of Fe(2+), Fe(3+), and Cu(2+). The PEP-FODs were derived from epinephrine via self-polymerization at relatively low temperature down to 60°C with low cytotoxicity and relative long lifetime (7.24ns). The surface morphology and optical properties of the synthesized PEP-FODs were characterized. We found that the diameters of PEP-FODs were mainly distributed in the narrow range of 2-4nm with an average diameter of 2.9nm. An optimal emission peak located at 490nm was observed when the green light-emitting PEP-FODs were excited at 400nm. It is discovered that Fe(2+), Fe(3+), and Cu(2+)can strongly quench the fluorescence of PEP-FODs through the nonradiative electron-transfer. The detection limit of 0.16, 0.67, and 0.15μM was obtained for Fe(2+), Fe(3+), and Cu(2+), respectively. The independent sensing platform of Fe(2+), Fe(3+), and Cu(2+)could be established by using NaF as a complexing agent and by regulating the reaction time between NaF and metal ions. Cell viability studies reveal that the as-prepared PEP-FODs possess good solubility and biocompatibility, making it as excellent imaging nanoprobes for intracellular Fe(2+), Fe(3+), and Cu(2+)sensing. The developed PEP-FODs might hold great promise to broaden applications in nanotechnology and bioanalysis. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Design and evaluation of capillary coupled with optical fiber light-emitting diode induced fluorescence detection for capillary electrophoresis.

    Science.gov (United States)

    Ji, Hongyun; Li, Meng; Guo, Lihong; Yuan, Hongyan; Wang, Chunling; Xiao, Dan

    2013-09-01

    A new detector, capillary coupled with optical fiber LED-induced fluorescence detector (CCOF-LED-IFD, using CCOF for short), is introduced for CE. The strategy of the present work was that the optical fiber and separation capillary were, in the parallel direction, fastened in a fixation capillary with larger inner diameter. By employing larger inner diameter, the fixation capillary allowed the large diameter of the optical fiber to be inserted into it. By transmitting an enhanced excitation light through the optical fiber, the detection sensitivity was improved. The advantages of the CCOF-CE system were validated by the detection of riboflavin, and the results were compared to those obtained by the in-capillary common optical fiber LED-induced fluorescence detector (IC-COF-LED-IFD, using COF for short). The LODs of CCOF-CE and COF-CE were 0.29 nM and 11.0 nM (S/N = 3), respectively. The intraday (n = 6) repeatability and interday (n = 6) reproducibility of migration time and corresponding peak area for both types of CE were all less than 1.10 and 3.30%, respectively. The accuracy of the proposed method was judged by employing standard addition method, and recoveries obtained were in the range of 98.0-102.4%. The results indicated that the sensitivity of the proposed system was largely improved, and that its reproducibility and accuracy were satisfactory. The proposed system was successfully applied to separate and determine riboflavin in real sample. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Determination of metallothioneins by fluorescence and resonance light scattering strategies based on ciprofloxacin–Cu(II) system

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Lu [College of Public Health, University of South China, Hengyang 421001 (China); Wang, Yong-Sheng, E-mail: yongsheng.w@tom.com [College of Public Health, University of South China, Hengyang 421001 (China); Xue, Jin-Hua; Yang, Hui-Xian; Li, Qiu; Zhou, Bin; Wang, Jia-Cheng; Yin, Ji-Cheng; Wang, Yong-Song [College of Public Health, University of South China, Hengyang 421001 (China); Xiao, Xi-Lin [College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001 (China)

    2013-06-15

    Based on ciprofloxacin (CIP)–Cu(II) system, the novel methods for the detection of metallothioneins (MTs) have been developed by fluorescence (FL) and resonance light scattering (RLS) strategies. The FL strategy avoids the label and derivatization steps in common methods, while the RLS strategy can be applied for determining bio-macromolecules and small molecules without native fluorescence. The response signals linearly correlated with the concentration of MTs over the ranges of 1.03×10{sup −8}–1.23×10{sup −6} mol L{sup −1} for FL, and of 2.56×10{sup −7}–1.54×10{sup −6} mol L{sup −1} for RLS. The limits of detection (LOD) are 3.1×10{sup −9} mol L{sup −1} for FL and 7.68×10{sup −8} mol L{sup −1} for RLS. This study represents the comparison of these two methods using the same CIP–Cu{sup 2+}–MTs system. They not only allow practical application for MTs detection but also serve as a potential choice for the operators according to their concrete needs. In addition, the mechanisms for FL and RLS enhancement of the system were also discussed. -- Highlights: ► Determination of MTs was developed based on CIP–Cu(II) system by FL and RLS strategies. ► FL strategy provides lower limit of detection and wider linear range, and avoids the label and derivatization steps. ► RLS strategy can be applied for determining bio-macromolecules and small molecules. ► The mechanism of interaction of MTs with CIP–Cu(II) chelate was discussed.

  2. An in vitro comparison of quantitative light-induced fluorescence-digital and spectrophotometer on monitoring artificial white spot lesions.

    Science.gov (United States)

    Kim, Hee Eun; Kim, Baek-Il

    2015-09-01

    The aim of this study was to evaluate the efficacy of quantitative light-induced fluorescence-digital (QLF-D) compared to a spectrophotometer in monitoring progression of enamel lesions. To generate artificial caries with various severities of lesion depths, twenty bovine specimens were immersed in demineralizing solution for 40 days. During the production of the lesions, repeat measurements of fluorescence loss (ΔF) and color change (ΔE) were performed in six distinct stages after the demineralization of the specimens: after 3, 5, 10, 20, 30, and 40 days of exposure to the demineralizing solution. Changes in the ΔF values in the lesions were analyzed using the QLF-D, and changes in the ΔE values in lesions were analyzed using a spectrophotometer. The repeated measures ANOVA of ΔF and ΔE values were used to determine whether there are significant differences at different exposure times in the demineralizing solution. Spearman's rank correlation coefficient was analyzed between ΔF and ΔE. The ΔF values significantly decreased based on the demineralizing period (p<0.001). Relatively large changes in ΔF values were observed during the first 10 days. There were significant changes in L(*), a(*), b(*), and ΔE values in lesions with increasing demineralizing duration (p<0.001). A strong correlation was observed between ΔF and ΔE with p=-0.853 (p<0.001). The results support the efficacy of QLF-D in monitoring color changes. Our findings demonstrate that QLF-D are a more efficient and stable tool for early caries detection. Copyright © 2015 Elsevier B.V. All rights reserved.

  3. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.

    Science.gov (United States)

    Takemoto, Kiwamu; Matsuda, Tomoki; Sakai, Naoki; Fu, Donald; Noda, Masanori; Uchiyama, Susumu; Kotera, Ippei; Arai, Yoshiyuki; Horiuchi, Masataka; Fukui, Kiichi; Ayabe, Tokiyoshi; Inagaki, Fuyuhiko; Suzuki, Hiroshi; Nagai, Takeharu

    2013-01-01

    Chromophore-assisted light inactivation (CALI) is a powerful technique for acute perturbation of biomolecules in a spatio-temporally defined manner in living specimen with reactive oxygen species (ROS). Whereas a chemical photosensitizer including fluorescein must be added to specimens exogenously and cannot be restricted to particular cells or sub-cellular compartments, a genetically-encoded photosensitizer, KillerRed, can be controlled in its expression by tissue specific promoters or subcellular localization tags. Despite of this superiority, KillerRed hasn't yet become a versatile tool because its dimerization tendency prevents fusion with proteins of interest. Here, we report the development of monomeric variant of KillerRed (SuperNova) by direct evolution using random mutagenesis. In contrast to KillerRed, SuperNova in fusion with target proteins shows proper localization. Furthermore, unlike KillerRed, SuperNova expression alone doesn't perturb mitotic cell division. Supernova retains the ability to generate ROS, and hence promote CALI-based functional analysis of target proteins overcoming the major drawbacks of KillerRed.

  4. Evaluation of Crawlspace Retrofits in Multifamily Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Rudd, Armin [Building Science Corporation, Somerville, MA (United States)

    2014-09-01

    In 2011 and early 2012, Building Science Corporation (BSC) collaborated with Innova Services Corporation on a multifamily community unvented crawlspace retrofit project at Oakwood Gardens in Lansdale, Pennsylvania. BSC provided design consulting services and pre- and post-retrofit evaluation, testing, and data monitoring. The existing condition was a vented crawlspace with an uninsulated floor between the crawlspace and the dwelling units above. The crawlspace was therefore a critically weak link in the building enclosure and was ripe for improvement. Saving energy was the primary interest and goal, but the greatest challenge in this unvented crawlspace retrofit project was working through a crawlspace bulk water intrusion problem caused by inadequate site drainage, window well drainage, foundation wall drainage, and a rising water table during rainy periods.

  5. Comparative study of commercial building energy-efficiency retrofit policies in four pilot cities in China

    International Nuclear Information System (INIS)

    Hou, Jing; Liu, Yisheng; Wu, Yong; Zhou, Nan; Feng, Wei

    2016-01-01

    The energy efficiency of existing commercial buildings is more challenging to regulate and improve than the energy efficiency of new constructions. In 2011 and 2012, the Chinese Government selected four cities- Shanghai, Tianjin, Shenzhen, and Chongqing- to implement pilot commercial building energy efficiency retrofit program. Based on site surveys and expert interviews in these pilot cities, this research conducted a comparative analysis on incentive policies of local city level. The analysis results show that policy designs of existing commercial buildings should be further improved. The aspects that influence the implementation effect in the future, such as subsidy level, installments, and business model promotion, should be specified in the policy clauses. Referring to the technical solution and cost-benefit in Chongqing, we found that lighting system is the most common retrofit objects while envelope system is the least common one. And the subsidy incentive is greatest for educational buildings, followed by office buildings. In the end, we further discussed the problems and obstacles in commercial building retrofit market, and provided a series of recommendations. - Highlights: • Data and information were collected through site surveys to the four pilot cities. • Policy design and effectiveness in four cities were comparatively analyzed. • Well-designed policy increases market response, energy savings and EMC adoption. • Lighting is the most common retrofit while envelope is the least common one. • Subsidy incentive is greatest for educational buildings due to the utility tariff.

  6. CH3NH3PbBr3Perovskite Nanocrystals as Efficient Light-Harvesting Antenna for Fluorescence Resonance Energy Transfer.

    Science.gov (United States)

    Muthu, Chinnadurai; Vijayan, Anuja; Nair, Vijayakumar C

    2017-05-04

    Hybrid perovskites have created enormous research interest as a low-cost material for high-performance photovoltaic devices, light-emitting diodes, photodetectors, memory devices and sensors. Perovskite materials in nanocrystal form that display intense luminescence due to the quantum confinement effect were found to be particularly suitable for most of these applications. However, the potential use of perovskite nanocrystals as a light-harvesting antenna for possible applications in artificial photosynthesis systems is not yet explored. In the present work, we study the light-harvesting antenna properties of luminescent methylammonium lead bromide (CH 3 NH 3 PbBr 3 )-based perovskite nanocrystals using fluorescent dyes (rhodamine B, rhodamine 101, and nile red) as energy acceptors. Our studies revealed that CH 3 NH 3 PbBr 3 nanocrystals are an excellent light-harvesting antenna, and efficient fluorescence resonance energy transfer occurs from the nanocrystals to fluorescent dyes. Further, the energy transfer efficiency is found to be highly dependent on the number of anchoring groups and binding ability of the dyes to the surface of the nanocrystals. These observations may have significant implications for perovskite-based light-harvesting devices and their possible use in artificial photosynthesis systems. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  7. Potential environmental impacts from the metals in incandescent, compact fluorescent lamp (CFL), and light-emitting diode (LED) bulbs.

    Science.gov (United States)

    Lim, Seong-Rin; Kang, Daniel; Ogunseitan, Oladele A; Schoenung, Julie M

    2013-01-15

    Artificial lighting systems are transitioning from incandescent to compact fluorescent lamp (CFL) and light-emitting diode (LED) bulbs in response to the U.S. Energy Independence and Security Act and the EU Ecodesign Directive, which leads to energy savings and reduced greenhouse gas emissions. Although CFLs and LEDs are more energy-efficient than incandescent bulbs, they require more metal-containing components. There is uncertainty about the potential environmental impacts of these components and whether special provisions must be made for their disposal at the end of useful life. Therefore, the objective of this study is to analyze the resource depletion and toxicity potentials from the metals in incandescent, CFL, and LED bulbs to complement the development of sustainable energy policy. We assessed the potentials by examining whether the lighting products are to be categorized as hazardous waste under existing U.S. federal and California state regulations and by applying life cycle impact-based and hazard-based assessment methods (note that "life cycle impact-based method" does not mean a general life cycle assessment (LCA) but rather the elements in LCA used to quantify toxicity potentials). We discovered that both CFL and LED bulbs are categorized as hazardous, due to excessive levels of lead (Pb) leachability (132 and 44 mg/L, respectively; regulatory limit: 5) and the high contents of copper (111,000 and 31,600 mg/kg, respectively; limit: 2500), lead (3860 mg/kg for the CFL bulb; limit: 1000), and zinc (34,500 mg/kg for the CFL bulb; limit: 5000), while the incandescent bulb is not hazardous (note that the results for CFL bulbs excluded mercury vapor not captured during sample preparation). The CFLs and LEDs have higher resource depletion and toxicity potentials than the incandescent bulb due primarily to their high aluminum, copper, gold, lead, silver, and zinc. Comparing the bulbs on an equivalent quantity basis with respect to the expected lifetimes of

  8. National Grid Deep Energy Retrofit Pilot

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, K. [Building Science Corporation (BSC), Somervgille, MA (United States)

    2012-03-01

    Through discussion of five case studies (test homes), this project evaluates strategies to elevate the performance of existing homes to a level commensurate with best-in-class implementation of high-performance new construction homes. The test homes featured in this research activity participated in Deep Energy Retrofit (DER) Pilot Program sponsored by the electric and gas utility National Grid in Massachusetts and Rhode Island. Building enclosure retrofit strategies are evaluated for impact on durability and indoor air quality in addition to energy performance.

  9. Retrofitting bus fleet for natural gas operation

    International Nuclear Information System (INIS)

    Stella, E.; Foresti, P.

    1992-01-01

    Buses, operating within a Florence (Italy) municipal transportation system, and equipped with Otto cycle engines, were selected for retrofitting taking into account the suitability of each vehicle's specific routing and service requirements. Cost benefit analyses indicated that it wouldn't be economically feasible to retrofit buses equipped with diesel engines. A computerized refuelling system was set up at the fleet's central service station which was hooked up to the natural gas utility's supply line. This paper tables the cost benefit analysis data comparing gasoline and methane operation and reflecting the cost savings which are expected to be accrued through this methanization program over a span of 14 years

  10. Advanced Energy Retrofit Guide Office Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Guopeng; Liu, Bing; Wang, Weimin; Zhang, Jian; Athalye, Rahul A.; Moser, Dave; Crowe, Eliot; Bengtson, Nick; Effinger, Mark; Webster, Lia; Hatten, Mike

    2011-09-27

    The Advanced Energy Retrofit Guide for Office Buildings is a component of the Department of Energy’s Advanced Energy Retrofit Guides for Existing Buildings series. The aim of the guides is to facilitate a rapid escalation in the number of energy efficiency projects in existing buildings and to enhance the quality and depth of those projects. By presenting general project planning guidance as well as financial payback metrics for the most common energy efficiency measures, these guides provide a practical roadmap to effectively planning and implementing performance improvements for existing buildings.

  11. Advanced Energy Retrofit Guide Retail Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Guopeng; Liu, Bing; Zhang, Jian; Wang, Weimin; Athalye, Rahul A.; Moser, Dave; Crowe, Eliot; Bengtson, Nick; Effinger, Mark; Webster, Lia; Hatten, Mike

    2011-09-19

    The Advanced Energy Retrofit Guide for Retail Buildings is a component of the Department of Energy’s Advanced Energy Retrofit Guides for Existing Buildings series. The aim of the guides is to facilitate a rapid escalation in the number of energy efficiency projects in existing buildings and to enhance the quality and depth of those projects. By presenting general project planning guidance as well as financial payback metrics for the most common energy efficiency measures, these guides provide a practical roadmap to effectively planning and implementing performance improvements for existing buildings.

  12. Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D).

    Science.gov (United States)

    Kim, Young-Seok; Lee, Eun-Song; Kwon, Ho-Keun; Kim, Baek-Il

    2014-06-01

    The aim of this study was to investigate whether Quantitative Light-induced Fluorescence-Digital (QLF-D) could monitor the degree of maturation of dental microcosm biofilms by observing the red fluorescence emitted from the biofilms. Dental microcosm the biofilms were grown on bovine enamel discs. They were initiated from human saliva, and then grown in 0.5% sucrose growth media for 10 days. On days 1, 2, 3, 7, and 10 after the inoculation, fluorescence images of the biofilms were captured using the QLF-D and the red fluorescence intensity was quantified by calculating the red/green ratio (R/G value). Total and aciduric bacteria within the biofilms were counted, and the degree of demineralization was evaluated by measuring the percentage of surface microhardness change (ΔVHN) and lesion depth in the enamel. The R/G values of the biofilms assessed by the QLF-D increased significantly over time up to 7 days after inoculation (pbiofilm maturation and was significantly associated with the cariogenicity of the biofilm. Therefore, this device could be used to monitor the degree of biofilm maturation by observing the red fluorescence emitted from cariogenic biofilms. The QLF-D enables the detection of a mature dental plaque and monitoring of its cariogenic status by observing the plaque fluorescence non-destructively, in real time. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. A New Photocatalytic System Using Steel Mesh and Cold Cathode Fluorescent Light for the Decolorization of Azo Dye Orange G

    Directory of Open Access Journals (Sweden)

    Ming-Chin Chang

    2012-01-01

    Full Text Available High color and organic composition, the effluents from the textile dyeing and finishing industry, can be treated by photocatalytic oxidation with UV/TiO2. The objective of this study was to prepare a new photocatalytic system by coating nanosized TiO2 particles on steel mesh support and using cold cathode fluorescent light (CCFL irradiation at 365 nm in a closed reactor for the oxidation of azo dye C.I. Orange G (OG. Various factors such as reaction time, coating temperature, TiO2 dosage, pH, initial dye concentration, and service duration were studied. Results showed efficient color removal of the OG azo dye by the photocatalytic system with TiO2-coated temperature at 150°C. The optimal TiO2 dosage for color removal was 60 g m−2. An acidic pH of 2.0 was sufficient for photocatalytic oxidation whereas basic condition was not. The rate of color removal decreased with increase in the initial dye concentration. The TiO2-coated steel mesh can be used repeatedly over 10 times without losing the photocatalytic efficiency. Results of FTIR and IC indicated the breakage of N=N bonds, with sulfate as the major and nitrite and nitrate as the minor products, which implied degradation of dye molecules.

  14. Non-rigid contour-to-pixel registration of photographic and quantitative light-induced fluorescence imaging of decalcified teeth

    Science.gov (United States)

    Berkels, Benjamin; Deserno, Thomas; Ehrlich, Eva E.; Fritz, Ulrike B.; Sirazitdinova, Ekaterina; Tatano, Rosalia

    2016-03-01

    Quantitative light-induced fluorescence (QLF) is widely used to assess the damage of a tooth due to decalcification. In digital photographs, decalcification appears as white spot lesions, i.e. white spots on the tooth surface. We propose a novel multimodal registration approach for the matching of digital photographs and QLF images of decalcified teeth. The registration is based on the idea of contour-to-pixel matching. Here, the curve, which represents the shape of the tooth, is extracted from the QLF image using a contour segmentation by binarization and morphological processing. This curve is aligned to the photo with a non-rigid variational registration approach. Thus, the registration problem is formulated as minimization problem with an objective function that consists of a data term and a regularizer for the deformation. To construct the data term, the photo is pointwise classified into tooth and non-tooth regions. Then, the signed distance function of the tooth region allows to measure the mismatch between curve and photo. As regularizer a higher order, linear elastic prior is used. The resulting minimization problem is solved numerically using bilinear Finite Elements for the spatial discretization and the Gauss-Newton algorithm. The evaluation is based on 150 image pairs, where an average of 5 teeth have been captured from 32 subjects. All registrations have been confirmed correctly by a dental expert. The contour-to-pixel methods can directly be used in 3D for surface-to-voxel tasks.

  15. Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair.

    Science.gov (United States)

    Packard, René R Sevag; Baek, Kyung In; Beebe, Tyler; Jen, Nelson; Ding, Yichen; Shi, Feng; Fei, Peng; Kang, Bong Jin; Chen, Po-Heng; Gau, Jonathan; Chen, Michael; Tang, Jonathan Y; Shih, Yu-Huan; Ding, Yonghe; Li, Debiao; Xu, Xiaolei; Hsiai, Tzung K

    2017-08-17

    This study sought to develop an automated segmentation approach based on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) to establish rapid reconstruction of the 3-D zebrafish cardiac architecture in response to doxorubicin-induced injury and repair. Input images underwent a 4-step automated image segmentation process consisting of stationary noise removal, histogram equalization, adaptive thresholding, and image fusion followed by 3-D reconstruction. We applied this method to 3-month old zebrafish injected intraperitoneally with doxorubicin followed by LSFI at 3, 30, and 60 days post-injection. We observed an initial decrease in myocardial and endocardial cavity volumes at day 3, followed by ventricular remodeling at day 30, and recovery at day 60 (P < 0.05, n = 7-19). Doxorubicin-injected fish developed ventricular diastolic dysfunction and worsening global cardiac function evidenced by elevated E/A ratios and myocardial performance indexes quantified by pulsed-wave Doppler ultrasound at day 30, followed by normalization at day 60 (P < 0.05, n = 9-20). Treatment with the γ-secretase inhibitor, DAPT, to inhibit cleavage and release of Notch Intracellular Domain (NICD) blocked cardiac architectural regeneration and restoration of ventricular function at day 60 (P < 0.05, n = 6-14). Our approach provides a high-throughput model with translational implications for drug discovery and genetic modifiers of chemotherapy-induced cardiomyopathy.

  16. Light emitting diodes versus compact fluorescent tubes for phototherapy in neonatal jaundice: a multi center randomized controlled trial.

    Science.gov (United States)

    Kumar, Praveen; Murki, Srinivas; Malik, G K; Chawla, Deepak; Deorari, Ashok K; Karthi, N; Subramanian, Sreeram; Sravanthi, Jonnala; Gaddam, Pramod; Singh, S N

    2010-02-01

    To evaluate whether light-emitting diode (LED) phototherapy is as efficacious as compact fluorescent tube (CFT) phototherapy for the treatment of non-hemolytic jaundice in healthy term and late preterm neonates. Multi centre open label randomized controlled trial. Four tertiary care neonatal units. Healthy term and late preterm neonates with non-hemolytic jaundice. Single-surface LED or CFT phototherapy. Duration of phototherapy. A total of 272 neonates were randomized to receive LED (n=142) or CFT (n=130) phototherapy. The baseline demographic and biochemical variables were similar in the two groups. The median duration of phototherapy (IQR) in the two groups was comparable (26 (22-36) h vs. 25(22-36) h; P=0.44). At any time point, a similar proportion of neonates were under phototherapy in the two groups (log-rank test, P=0.38). The rate of fall of serum total bilirubin (STB) during phototherapy and the incidence of failure of phototherapy were also not different. An equal proportion of neonates had a rebound increase in STB needing restarting of phototherapy. Side effects were rare, comparable in the two groups and included hypothermia, hyperthermia, rash, skin darkening and dehydration. LED and CFT phototherapy units were equally efficacious in the management of non-hemolytic hyperbilirubinemia in healthy term and late preterm neonates.

  17. Polarity-Tunable Host Materials and Their Applications in Thermally Activated Delayed Fluorescence Organic Light-Emitting Diodes.

    Science.gov (United States)

    Xie, Gaozhan; Chen, Dongjun; Li, Xianglong; Cai, Xinyi; Li, Yunchuan; Chen, Dongcheng; Liu, Kunkun; Zhang, Qian; Cao, Yong; Su, Shi-Jian

    2016-10-04

    A series of polarity-tunable host materials were developed based on oligocarbazoles and diphenylphosphine oxide, and their polarities can be tuned through increasing distance of acceptor and donor units. Density functional theory calculations were employed, and photoluminescence spectra in different polar solvents were measured to illustrate different polarities of these host materials. As CZPO has relatively stronger polarity, electroluminescence (EL) spectrum of solution-processed device based on 6 wt % PXZDSO2:CZPO is 7 nm red-shifted relative to that of other host materials based devices. Besides, a comparable impressive external quantum efficiency (EQE) value of 18.7% is achieved for an evaporation-processed yellow device consisting of FCZBn, which is superior to that of the device based on CBP (4,4'-dicarbazolyl-1,1'-biphenyl) (17.0%), and its efficiency roll-off is also obviously reduced, giving an EQE value as high as 16.3% at the luminance of 1000 cd/m 2 . In addition, from CZPO to FCZBn as the polarities of host materials decrease, EL spectra of solution-processed devices based on DMAC-DPS emitter blue-shift constantly from 496 to 470 nm. The current work gives a constructive approach to control EL spectra of organic light-emitting diodes with a fixed thermally activated delayed fluorescence emitter by tuning the polarities of host materials.

  18. Micro-X-ray fluorescence spectrometer with x-ray single bounce metallic capillary optics for light element analysis (Conference Presentation)

    Science.gov (United States)

    Mroczka, Robert; Żukociński, Grzegorz; Łopucki, Rafał

    2017-05-01

    In the last 20 years, , due to the rapid development of X-ray optics, micro X-ray fluorescence spectrometry (micro-XRF) has become a powerful tool to determine the spatial distribution of major, minor, and trace elements within a sample. Micro-X-ray fluorescence (micro-XRF) spectrometers for light element analysis (6 work was supported and co-funded by the European Union as part of the Operational Programme Development of Eastern Poland for 2007-2013, Priority I Innovative Economy, Measure I.3. Support for Innovations and The National Centre for Research and Development, Project no. TANGO1,267102/NCBR/2015

  19. Diesel retrofit assessment for NYS DOT to retrofit its existing engine fleet.

    Science.gov (United States)

    2010-08-01

    The NYS DEC has required the use of retrofit technologies for various state agency, state public authority, and regional public authority heavy duty vehicles, as well as heavy duty vehicles used on behalf of such agencies and authorities. This report...

  20. Greenbuilt Retrofit Test House Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Sparn, B.; Hudon, K.; Earle, L.; Booten, C.; Tabares-Velasco, P. C.; Barker, G.; Hancock, C. E.

    2014-06-01

    The Greenbuilt house, is an all-electric, 1980's era home in the eastern Sacramento suburb of Fair Oaks that was retrofit by Greenbuilt Construction as part of Sacramento Municipal Utility District's (SMUD) Energy Efficient Remodel Demonstration (EERD) Program. The project was a joint effort between the design-build team at Greenbuilt Construction, led by Jim Bayless, SMUD and their project manager Mike Keesee, and the National Renewable Energy Laboratory (NREL). The goal of the Energy Efficient Remodel Demonstration program is to work with local builders to renovate homes with cost-effective energy efficient retrofit measures. The homes remodeled under the EERD program are intended to showcase energy efficient retrofit options for homeowners and other builders. The Greenbuilt house is one of five EERD projects that NREL has supported. NREL's main role in these projects is to provide energy analysis and to monitor the home's performance after the retrofit to verify that the energy consumption is in line with the modeling predictions. NREL also performed detailed monitoring on the more innovative equipment included in these remodels, such as an add-on heat pump water heater.

  1. Retrofitting the 5045 Klystron for Higher Efficiency

    International Nuclear Information System (INIS)

    Jensen, Aaron; Fazio, Michael; Haase, Andy; Jongewaard, Erik; Kemp, Mark; Neilson, Jeff

    2015-01-01

    The 5045 klystron has been in production and accelerating particles at SLAC National Accelerator Laboratory for over 25 years. Although the design has undergone some changes there are still significant opportunities for improvement in performance. Retrofitting the 5045 for higher efficiencies and a more mono-energetic spent beam profile is presented.

  2. National Grid Deep Energy Retrofit Pilot

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, K.

    2012-03-01

    Through discussion of five case studies (test homes), this project evaluates strategies to elevate the performance of existing homes to a level commensurate with best-in-class implementation of high-performance new construction homes. The test homes featured in this research activity participated in Deep Energy Retrofit (DER) Pilot Program sponsored by the electric and gas utility National Grid in Massachusetts and Rhode Island. Building enclosure retrofit strategies are evaluated for impact on durability and indoor air quality in addition to energy performance. Evaluation of strategies is structured around the critical control functions of water, airflow, vapor flow, and thermal control. The aim of the research project is to develop guidance that could serve as a foundation for wider adoption of high performance, 'deep' retrofit work. The project will identify risk factors endemic to advanced retrofit in the context of the general building type, configuration and vintage encountered in the National Grid DER Pilot. Results for the test homes are based on observation and performance testing of recently completed projects. Additional observation would be needed to fully gauge long-term energy performance, durability, and occupant comfort.

  3. Dowel bar retrofit performance in Wisconsin.

    Science.gov (United States)

    2010-05-01

    In 1999, WisDOT constructed test sections on I-39 to evaluate the dowel bar retrofit (DBR) rehabilitation technique for faulted concrete pavement slabs. Two years later, mortar deterioration and debonding were noted in the dowel slots. In response to...

  4. Retrofitting unreinforced masonry | Ngowi | Botswana Journal of ...

    African Journals Online (AJOL)

    This paper is expected to provide general guidelines to practicing Engineers to assist them in deciding the appropriate alternatives in strengthening unreinforced masonry structures. Key Words: Unreinforced masonry, structural strengthening, Fibre Reinforced Polymer, Earthquake, retrofitting, wind. Botswana Journal of ...

  5. Underwater Adhesives Retrofit Pipelines with Advanced Sensors

    Science.gov (United States)

    2015-01-01

    Houston-based Astro Technology Inc. used a partnership with Johnson Space Center to pioneer an advanced fiber-optic monitoring system for offshore oil pipelines. The company's underwater adhesives allow it to retrofit older deepwater systems in order to measure pressure, temperature, strain, and flow properties, giving energy companies crucial data in real time and significantly decreasing the risk of a catastrophe.

  6. DIESEL ENGINE RETROFIT TECHNOLOGY VERIFICATION (POSTER)

    Science.gov (United States)

    ETV is presenting a poster at the EPA's 2005 Science Forum from May 16-18, 2005 in Washington, DC. This poster will contain a summary of the performance results realized by the six verified diesel retrofit technologies, as well as potential impacts that could be realized if sigi...

  7. Sustainable Retrofitting of Nordic University Campuses

    DEFF Research Database (Denmark)

    Eriksson, Robert; Nenonen, Suvi; Nielsen, Susanne Balslev

    2014-01-01

    Purpose The purpose of this paper is to identify the state-of-art of Nordic campus development and identify how campus areas can be retrofitted by addition of new technologies, features, functions and services. The leading research question is: how to develop Nordic resilient campus management...

  8. Evaluation of Crawlspace Retrofits in Multifamily Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Rudd, A.

    2014-09-01

    In 2011 and early 2012, Building Science Corporation (BSC) collaborated with Innova Services Corporation on a multifamily community unvented crawlspace retrofit project at Oakwood Gardens in Lansdale, Pennsylvania. BSC provided design consulting services and pre- and post-retrofit evaluation, testing, and data monitoring. The existing condition was a vented crawlspace with an uninsulated floor between the crawlspace and the dwelling units above. The crawlspace was therefore a critically weak link in the building enclosure and was ripe for improvement. Saving energy was the primary interest and goal, but the greatest challenge in this unvented crawlspace retrofit project was working through a crawlspace bulk water intrusion problem caused by inadequate site drainage, window well drainage, foundation wall drainage, and a rising water table during rainy periods. While the unvented crawlspace retrofit was effective in reducing heat loss, and the majority of the bulk water drainage problems had been resolved, the important finding was that some of the wood joists embedded in masonry pockets behind the brick veneer were showing signs of moisture damage.

  9. Homeowner Best Practices Guide for Residential Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain S.

    2005-09-01

    This best practices guide for HV AC system retrofits is aimed at homeowners who want guidance on upgrading their heating, cooling and ventilation (HVAC) systems and integrating these upgrades with other changes to their home. It has been developed around the idea of having packages of changes to the building HV AC system and building envelope that are climate and house construction dependent. These packages include materials procedures and equipment, and are designed to remove some of the guesswork when selecting a builder, contractor, or installer. The packages are not meant to be taken as rigid requirements - instead they are systems engineered guidelines that form the basis for energy efficient retrofits. Similar approaches have been taken previously for new construction, where a systems engineering approach has been used to develop extremely energy-efficient homes that are comfortable safe and durable, and often cost less than standard construction. This approach is best epitomized by the Building America program, whose partners have built thousands of residences throughout the U.S. using these principles. The differences between retrofitting and new construction tend to limit the changes one can make to a building, so these packages rely on relatively simple and non-intrusive technologies and techniques. The retrofits also focus on changes to a building that will give many years of service to the occupants. Another key aspect of these best practices is that we need to know how a house is working so that we know what parts have the potential for improvement. To do this we have put together a set of simple tests that a homeowner can perform on their own together with checklists and questionnaires. The measured test results, observations and homeowner answers to questions are used to direct us towards the best retrofits applicable to each individual house. The retrofits will depend on the current condition of the building envelope and HV AC system, the local

  10. Best practices guide for residential HVAC Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain S.

    2003-08-11

    This best practices guide for residential HVAC system retrofits is aimed at contractors who want guidance on delivering energy efficient, cost effective and innovative products. It has been developed around the idea of having packages of changes to the building HVAC system and building envelope that are climate and house construction dependent. These packages include materials, procedures and equipment and are designed to remove some of the guesswork from a builder, contractor, installer or homeowner decisions about how best to carry out HVAC changes. The packages are not meant to be taken as rigid requirements--instead they are systems engineered guidelines that form the basis for energy efficient retrofits. Similar approaches have been taken previously for new construction to develop extremely energy efficient homes that are comfortable safe and durable, and often cost less than standard construction. This is best epitomized by the Building America program whose partners have built thousands of residences throughout the U.S. using these principles. The differences between retrofitting and new construction tend to limit the changes one can make to a building, so these packages rely on relatively simple and non-intrusive technologies and techniques. The retrofits also focus on changes to a building that will give many years of service to the occupants. Another key aspect of these best practices is that we need to know how a house is working so that we know what parts have the potential for improvement. To do this we have put together a set of diagnostic tools that combine physical measurements and checklists/questionnaires. The measured test results, observations and homeowner answers to questions are used to direct us towards the best retrofits applicable to each individual house. The retrofits will depend on the current condition of the building envelope and HVAC system, the local climate, the construction methods used for the house, and the presence of various

  11. Bay Ridge Gardens - Mixed-Humid Affordable Multifamily Housing Deep Energy Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Lyons, J.; Moore, M.; Thompson, M.

    2013-08-01

    Under this project, Newport Partners (as part of the BA-PIRC research team) evaluated the installation, measured performance, and cost-effectiveness of efficiency upgrade measures for a tenant-in-place DER at the Bay Ridge multifamily (MF) development in Annapolis, Maryland. The design and construction phase of the Bay Ridge project was completed in August 2012. This report summarizes system commissioning, short-term test results, utility bill data analysis, and analysis of real-time data collected over a one-year period after the retrofit was complete. The Bay Ridge project is comprised of a 'base scope' retrofit which was estimated to achieve a 30%+ savings (relative to pre-retrofit) on 186 apartments, and a 'DER scope' which was estimated to achieve 50% savings (relative to pre-retrofit) on a 12-unit building. The base scope was applied to the entire apartment complex, except for one 12-unit building which underwent the DER scope. A wide range of efficiency measures was applied to pursue this savings target for the DER building, including improvements/replacements of mechanical equipment and distribution systems, appliances, lighting and lighting controls, the building envelope, hot water conservation measures, and resident education. The results of this research build upon the current body of knowledge of multifamily retrofits. Towards this end, the research team has collected and generated data on the selection of measures, their estimated performance, their measured performance, and risk factors and their impact on potential measures.

  12. Effect of Different Contact Materials on Approximal Caries Detection by Laser Fluorescence and Light-Emitting Diode Devices.

    Science.gov (United States)

    Ozsevik, A Semih; Kararslan, Emine Sirin; Aktan, Ali Murat; Bozdemir, Esin; Cebe, Fatma; Sarı, Fatih

    2015-10-01

    The aim of this in vitro study was to investigate the influence of the adjacent tooth surface on pen type laser fluorescence (LFpen) and light-emitting diode (LED) device readings in detecting approximal caries lesions in permanent teeth. Early detection of noncavitated dental caries is important, because disease progression can be easily halted at this stage with certain applications, such as fluoride therapy, antibacterial therapy, dietary changes, or low-intensity laser irradiation. A total of 87 permanent molars with 156 approximal surfaces were assessed with LED- and LF-based devices in contact with sound tooth surfaces (the control group) as well as approximal amalgam, composite, zirconia, and full ceramic restorations. All teeth were assessed once by one trained examiner. After the LF and LED assessments, the teeth were histologically evaluated using stereomicroscopy as the gold standard. The sensitivity, specificity, accuracy, and area under the receiver operating characteristics (ROC) curve were calculated according to the appropriate thresholds (T1, sound surface or enamel caries; T2, dentin caries). For the LFpen device, higher sensitivity and accuracy was found when the adjacent surface was sound at the T1 threshold and no significant differences were found among accuracy at the T2 threshold. For the LED-based device, no significant differences were found among sensitivities at the T1 threshold. At the T2 threshold, specificity was higher when the adjacent tooth had a zirconia restoration. It was found that both devices could be used effectively for evaluating approximal surfaces with adjacent restored teeth, regardless of the different kinds of restorative materials.

  13. Assessment of dental plaque coverage by Quantitative Light-induced Fluorescence (QLF) in domestic short-haired cats.

    Science.gov (United States)

    Marshall-Jones, Zoe V; Wallis, Corrin V; Allsopp, Judi M; Colyer, Alison; Davis, Ian J; Holcombe, Lucy J

    2017-04-01

    Dietary means of reducing plaque and calculus deposits are frequently sought for the maintenance of oral health in cats and dogs. In the development of such products sensitive, reliable, reproducible methods of measuring plaque and calculus are key. The aim of this study was to assess Quantitative Light-induced Fluorescence (QLF™) for the detection of dental plaque coverage in cats compared to the modified Logan and Boyce technique. The techniques were utilised in a crossover study, which compared two diets for their effect on plaque deposition in a cohort of 24 adult cats. Analysis of the effect of diet on plaque coverage by both the modified Logan and Boyce technique and QLF showed a significant effect of feeding regime (p=0.024 and p≤0.0001, respectively) with good agreement between the techniques in the percentage reduction of plaque accumulation. A within study assessment of QLF demonstrated excellent intra-operator repeatability (coefficient of variation 2.2%). Similarly, inter-operator reproducibility was also good (coefficient of variation 2.3%). A retrospective analysis, using the data to estimate the sample size required for at least 90% power to detect a 15% difference between treatments in a two-way crossover study, established that 10 cats would be sufficient for plaque measurement by QLF, while assessment by the modified Logan and Boyce method required over 30 cats. QLF was determined to be a reliable, reproducible method for the assessment of plaque deposition in cats and requires fewer subjects for the detection of differences between treatment effects compared to the modified Logan and Boyce method. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  14. Validation of quantitative light-induced fluorescence-digital (QLF-D) for the detection of approximal caries in vitro.

    Science.gov (United States)

    Ko, Hae-Youn; Kang, Si-Mook; Kim, Hee Eun; Kwon, Ho-Keun; Kim, Baek-Il

    2015-05-01

    Detection of approximal caries lesions can be difficult due to their anatomical position. This study aimed to assess the ability of the quantitative light-induced fluorescence-digital (QLF-D) in detecting approximal caries, and to compare the performance with those of the International Caries Detection and Assessment System II (ICDAS II) and digital radiography (DR). Extracted permanent teeth (n=100) were selected and mounted in pairs. The simulation pairs were assessed by one calibrated dentist using each detection method. After all the examinations, the teeth (n=95) were sectioned and examined histologically as gold standard. The modalities were compared in terms of sensitivity, specificity, areas under receiver operating characteristic curves (AUROC) for enamel (D1) and dentine (D3) levels. The intra-examiner reliability was assessed for all modalities. At D1 threshold, the ICDAS II presented the highest sensitivity (0.80) while the DR showed the highest specificity (0.89); however, the methods with the greatest AUC values at D1 threshold were DR and QLF-D (0.80 and 0.80 respectively). At D3 threshold, the methods with the highest sensitivity were ICDAS II and QLF-D (0.64 and 0.64 respectively) while the method with the lowest sensitivity was DR (0.50). However, with regard to the AUC values at D3 threshold, the QLF-D presented the highest value (0.76). All modalities showed to have excellent intra-examiner reliability. The newly developed QLF-D was not only able to detect proximal caries, but also showed to have comparable performance to the visual inspection and radiography in detecting proximal caries. QLF-D has the potential to be a useful detection method for proximal caries. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Comparison of intensive light-emitting diode and intensive compact fluorescent phototherapy in non-hemolytic jaundice.

    Science.gov (United States)

    Takcı, Sahin; Yiğit, Sule; Bayram, Gülperi; Korkmaz, Ayşe; Yurdakök, Murat

    2013-01-01

    In severe and rapidly increasing jaundice, the use of intensive phototherapy provides greater effectiveness and a faster decrement in bilirubin levels compared to conventional phototherapy. The aim of this study was to compare the effectiveness of two types of intensive phototherapy: intensive compact fluorescent tube (CFT) and intensive light-emitting diode (LED) phototherapy. Forty-three infants over 35 weeks of gestation with severe non-hemolytic hyperbilirubinemia were enrolled in the prospective study. All infants received multidirectional (circular-shaped) intensive phototherapy. Of these, 20 infants received CFT while 23 infants received LED phototherapy. Bilirubin levels and body temperatures were measured periodically, and the rates of bilirubin decrement were calculated. Mean serum bilirubin level of the 43 infants was 20.5±1.5 mg/dl at the beginning of the therapy and mean duration of phototherapy was 20.6±1.1 hours. The rate of mean bilirubin decline was 47.2% and the decrease was more prominent in the first four hours (0.84 ± 0.41 mg/dl/h). The rates of bilirubin decrement were comparable between the LED and CFT groups. Slightly elevated mean body temperature (37.1ºC) was determined in the CFT group (pphototherapy units with both LED and CFT were effective, showing a decline of half the initial value of bilirubin during the study period in infants with non-hemolytic jaundice. This study shows that intensive phototherapy with either CFT or LED can provide rapid decrease in bilirubin levels in the first few hours. This rapid decline is important in cases that have high risk of bilirubin encephalopathy.

  16. A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source

    OpenAIRE

    Pompidor, Guillaume; Dworkowski, Florian S. N.; Thominet, Vincent; Schulze-Briese, Clemens; Fuchs, Martin R.

    2013-01-01

    The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of the in situ on-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis ab...

  17. Highly Efficient Orange and Red Phosphorescent Organic Light-Emitting Diodes with Low Roll-Off of Efficiency using a Novel Thermally Activated Delayed Fluorescence Material as Host.

    Science.gov (United States)

    Wang, Hui; Meng, Lingqiang; Shen, Xingxing; Wei, Xiaofang; Zheng, Xiuli; Lv, Xiaopeng; Yi, Yuanping; Wang, Ying; Wang, Pengfei

    2015-07-15

    MTXSFCz with thermally activated delayed fluorescence is synthesized. Orange and red phosphorescent organic light-emitting diodes (PHOLEDs) with low efficiency roll-off exhibit external quantum efficiencies (EQE) up to 11.8% and 15.6%. The efficient upconversion from triplet to singlet of the host reduces the triplet density and thus affords a low efficiency roll-off of PHOLEDs. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Increasing fluorescent concentrator light collection efficiency by restricting the angular emission characteristic of the incorporated luminescent material: the 'Nano-Fluko' concept

    Science.gov (United States)

    Goldschmidt, J. C.; Peters, M.; Gutmann, J.; Steidl, L.; Zentel, R.; Bläsi, B.; Hermle, M.

    2010-05-01

    Fluorescent concentrators concentrate both diffuse and direct radiation without requiring tracking of the sun. In fluorescent concentrators, luminescent materials embedded in a transparent matrix absorb sunlight and emit radiation with a different wavelength. Total internal reflection traps the emitted light and guides it to solar cells attached to the concentrator's edges. The escape cone of total internal reflection, however, limits the light collection efficiency. Spectrally selective photonic structures, which transmit light in the absorption range of the luminescent material and reflect the emitted light, reduce these losses. In this paper, we review different realizations of such structures and show that they increase collection efficiency by 20%. However, light emitted into steep angles in respect to the front surface, which would be lost without the photonic structures, has a very long effective path inside the concentrator until it reaches a solar cell. Therefore it suffers from path length dependent losses. We discuss how emission into the unfavorable directions can be suppressed by integrating the luminescent material into photonic structures, thus reducing these losses. We present possible realizations both for the concentrator design and for the solar cells used in such systems.

  19. Quantitative Light-induced Fluorescence-Digital as an oral hygiene evaluation tool to assess plaque accumulation and enamel demineralization in orthodontics.

    Science.gov (United States)

    Miller, Cara C; Burnside, Girvan; Higham, Susan M; Flannigan, Norah L

    2016-11-01

      To assess the use of Quantitative Light-induced Fluorescence-Digital as an oral hygiene evaluation tool during orthodontic treatment.   In this prospective, randomized clinical trial, 33 patients undergoing fixed orthodontic appliance treatment were randomly allocated to receive oral hygiene reinforcement at four consecutive appointments using either white light (WL) or Quantitative Light-induced Fluorescence-Digital (QLF) images, taken with a device, as visual aids. Oral hygiene was recorded assessing the QLF images for demineralization, by fluorescence loss (ΔF), and plaque coverage (ΔR30). A debriefing questionnaire ascertained patient perspectives.   There were no significant differences in demineralization (P  =  .56) or plaque accumulation (P  =  .82) between the WL and QLF groups from T0 to T4. There was no significant reduction in demineralization, ΔF, in the WL, or the QLF group from T0-T4 (P > .05); however, there was a significant reduction in ΔR30 plaque scores (P oral hygiene reinforcement for the full duration of treatment compared with 81% of the WL group (OR 2.3; P Oral hygiene reinforcement at consecutive appointments using WL or QLF images as visual aids is effective in reducing plaque coverage. In terms of clinical benefits, QLF and WL images are of similar effectiveness; however, patients preferred the QLF images.

  20. Contribution to the analysis of light elements using x fluorescence excited by radio-elements; Contribution a l'analyse des elements legers par fluorescence x excitee au moyen de radioelements

    Energy Technology Data Exchange (ETDEWEB)

    Robert, A. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    In order to study the possibilities of using radioactive sources for the X-fluorescence analysis of light elements, the principle is given, after a brief description of X-fluorescence, of the excitation of this phenomenon by X, {beta} and {alpha} emission from radio-elements. The operation and use of the proportional gas counter for X-ray detection is described. A device has been studied for analysing the elements of the 2. and 3. periods of the Mendeleev table. It makes it possible to excite the fluorescence with a radioactive source emitting X-rays or a particles; the X-ray fluorescence penetrates into a window-less proportional counter, this being made possible by the use of an auxiliary electric field in the neighbourhood of the sample. The gas detection pressure leading to the maximum detection yield is given. The spectra are given for the K{sub {alpha}} lines of 3. period elements excited by {sup 55}Fe, {sup 3}H/Zr and {sup 210}Po sources; for the 2. period the K{sub {alpha}} spectra of carbon and of fluorine excited by the {alpha} particles of {sup 210}Po. (author) [French] Afin d'etudier les possibilites d'emploi de sources radioactives a l'analyse par fluorescence X des elements legers, on presente apres rappel de notions generales sur la fluorescence X, le principe de l'excitation de ce phenomene par emission X, {beta}, {alpha} de radioelements. Le fonctionnement et l'utilisation du compteur proportionnel a gaz a la detection du rayonnement X est developpe. Un dispositif permettant l'analyse des elements des 2eme et 3eme periodes de la classification de Mendeleev est etudie. Il permet l'excitation de la fluorescence par source radioactive emettrice de rayons X ou de particules {alpha}; le rayonnement X de fluorescence penetre dans un compteur proportionnel depourvu de fenetre, ceci est rendu possible en creant un champ electrique auxiliaire au voisinage de l'echantillon. On definit une pression du gaz de detection

  1. High Power Solid State Retrofit Lamp Thermal Characterization and Modeling

    Directory of Open Access Journals (Sweden)

    J. Jakovenko

    2012-04-01

    Full Text Available Thermal and thermo-mechanical modeling and characterization of solid state lightening (SSL retrofit LED lamp are presented in this paper. Paramount importance is to design SSL lamps for reliability, in which thermal and thermo-mechanical aspects are key points. The main goal is to get a precise 3D thermal lamp model for further thermal optimization. Simulations are performed with ANSYS and CoventorWare software tools to compere different simulation approaches. Simulated thermal distribution has been validated with thermal measurement on a commercial 8W LED lamp. Materials parametric study has been carried out to discover problematic parts for heat transfer from power LEDs to ambient and future solutions are proposed. The objectives are to predict the thermal management by simulation of LED lamp, get more understanding in the effect of lamp shape and used materials in order to design more effective LED lamps and predict light quality, life time and reliability.

  2. Lighting

    Data.gov (United States)

    Federal Laboratory Consortium — Lighting Systems Test Facilities aid research that improves the energy efficiency of lighting systems. • Gonio-Photometer: Measures illuminance from each portion of...

  3. Evaluation of an LED Retrofit Project at Princeton University's Carl Icahn Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Robert [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Murphy, Arthur [Princeton Univ., NJ (United States); Perrin, Tess [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2015-11-01

    At Princeton University’s Carl Icahn Laboratory, DOE’s Commercial Buildings Integration Program documented the implementation of LED retrofit products for recessed troffers, linear cove lighting, and downlights – as part of Princeton’s first building-wide interior LED project. The conversion to LED enables more extensive use of lighting controls to tailor the lighting to the task and limit the operating hours based on occupancy, and the estimated energy savings including controls is 62% compared to the incumbent system.

  4. Light

    DEFF Research Database (Denmark)

    Prescott, N.B.; Kristensen, Helle Halkjær; Wathes, C.M.

    2004-01-01

    This chapter presents the effect of artificial light environments (light levels, colour, photoperiod and flicker) on the welfare of broilers in terms of vision, behaviour, lameness and mortality......This chapter presents the effect of artificial light environments (light levels, colour, photoperiod and flicker) on the welfare of broilers in terms of vision, behaviour, lameness and mortality...

  5. Shining a Light on Savings.

    Science.gov (United States)

    Kennedy, Mike

    2000-01-01

    Discusses how schools and universities can save energy and money by evaluating lighting systems and changing behaviors. Retrofitting older buildings with better lighting technology and use of natural light are examined. An example of an energy conservation education program to reduce energy waste is highlighted. (GR)

  6. Fluorescent protein-mediated colour polymorphism in reef corals: multicopy genes extend the adaptation/acclimatization potential to variable light environments.

    Science.gov (United States)

    Gittins, John R; D'Angelo, Cecilia; Oswald, Franz; Edwards, Richard J; Wiedenmann, Jörg

    2015-01-01

    The genomic framework that enables corals to adjust to unfavourable conditions is crucial for coral reef survival in a rapidly changing climate. We have explored the striking intraspecific variability in the expression of coral pigments from the green fluorescent protein (GFP) family to elucidate the genomic basis for the plasticity of stress responses among reef corals. We show that multicopy genes can greatly increase the dynamic range over which corals can modulate transcript levels in response to the light environment. Using the red fluorescent protein amilFP597 in the coral Acropora millepora as a model, we demonstrate that its expression increases with light intensity, but both the minimal and maximal gene transcript levels vary markedly among colour morphs. The pigment concentration in the tissue of different morphs is strongly correlated with the number of gene copies with a particular promoter type. These findings indicate that colour polymorphism in reef corals can be caused by the environmentally regulated expression of multicopy genes. High-level expression of amilFP597 is correlated with reduced photodamage of zooxanthellae under acute light stress, supporting a photoprotective function of this pigment. The cluster of light-regulated pigment genes can enable corals to invest either in expensive high-level pigmentation, offering benefits under light stress, or to rely on low tissue pigment concentrations and use the conserved resources for other purposes, which is preferable in less light-exposed environments. The genomic framework described here allows corals to pursue different strategies to succeed in habitats with highly variable light stress levels. In summary, our results suggest that the intraspecific plasticity of reef corals' stress responses is larger than previously thought. © 2014 The Authors Molecular Ecology Published by John Wiley & Sons Ltd.

  7. Cascade Apartments: Deep Energy Multifamily Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Gordon, A. [Washington State Univ. Energy Program, Olympia, WA (United States); Mattheis, L. [Washington State Univ. Energy Program, Olympia, WA (United States); Kunkle, R. [Washington State Univ. Energy Program, Olympia, WA (United States); Howard, L. [Washington State Univ. Energy Program, Olympia, WA (United States); Lubliner, M. [Washington State Univ. Energy Program, Olympia, WA (United States)

    2014-02-01

    In December of 2009-10, King County Housing Authority (KCHA) implemented energy retrofit improvements in the Cascade multifamily community, located in Kent, Washington (marine climate.)This research effort involved significant coordination from stakeholders KCHA, WA State Department of Commerce, utility Puget Sound Energy, and Cascade tenants. This report focuses on the following three primary BA research questions: 1. What are the modeled energy savings using DOE low income weatherization approved TREAT software? 2. How did the modeled energy savings compare with measured energy savings from aggregate utility billing analysis? 3. What is the Savings to Investment Ratio (SIR) of the retrofit package after considering utility window incentives and KCHA capitol improvement funding.

  8. Retrofit California Overview and Final Reports

    Energy Technology Data Exchange (ETDEWEB)

    Choy, Howard; Rosales, Ana

    2014-03-01

    Energy efficiency retrofits (also called upgrades) are widely recognized as a critical component to achieving energy savings in the building sector to help lower greenhouse gas (GHG) emissions. To date, however, upgrades have accounted for only a small percentage of aggregate energy savings in building stock, both in California and nationally. Although the measures and technologies to retrofit a building to become energy efficient are readily deployed, establishing this model as a standard practice remains elusive. Retrofit California sought to develop and test new program models to increase participation in the energy upgrade market in California. The Program encompassed 24 pilot projects, conducted between 2010 and mid-2013 and funded through a $30 million American Recovery and Reinvestment Act (ARRA) grant from the U.S. Department of Energy’s (DOE) Better Buildings Neighborhood Program (BBNP). The broad scope of the Program can be seen in the involvement of the following regionally based Grant Partners: Los Angeles County (as prime grantee); Association of Bay Area Governments (ABAG), consisting of: o StopWaste.org for Alameda County o Regional Climate Protection Authority (RCPA) for Sonoma County o SF Environment for the City and County of San Francisco o City of San Jose; California Center for Sustainable Energy (CCSE) for the San Diego region; Sacramento Municipal Utilities District (SMUD). Within these jurisdictions, nine different types of pilots were tested with the common goal of identifying, informing, and educating the people most likely to undertake energy upgrades (both homeowners and contractors), and to provide them with incentives and resources to facilitate the process. Despite its limited duration, Retrofit California undoubtedly succeeded in increasing awareness and education among home and property owners, as well as contractors, realtors, and community leaders. However, program results indicate that a longer timeframe will be needed to

  9. Cascade Apartments: Deep Energy Multifamily Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Gordon, A. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Mattheis, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Kunkle, R. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Howard, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Lubliner, M. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-02-01

    In December of 2009-10, King County Housing Authority (KCHA) implemented energy retrofit improvements in the Cascade multifamily community, located in Kent, Washington (marine climate.)This research effort involved significant coordination from stakeholders KCHA, WA State Department of Commerce, utility Puget Sound Energy, and Cascade tenants. This report focuses on the following three primary BA research questions : 1. What are the modeled energy savings using DOE low income weatherization approved TREAT software? 2. How did the modeled energy savings compare with measured energy savings from aggregate utility billing analysis? 3. What is the Savings to Investment Ratio (SIR) of the retrofit package after considering utility window incentives and KCHA capitol improvement funding.

  10. Fluorescent minerals--A potential source of UV protection and visible light for the growth of green algae and cyanobacteria in extreme cosmic environments.

    Science.gov (United States)

    Omairi, Tareq; Wainwright, Milton

    2015-07-01

    We propose that green algae (Chlorella variabilis and Dunaliella tertiolecta) and cyanobacteria (Synechococcus elongatus and Nostoc commune) can grow inside fluorescent rock minerals which convert damaging UV light to visible light, thereby allowing these organisms to survive and thrive in UV-rich environments without (or with limited) visible light, which would otherwise be inimical to them. The four microorganisms were incubated inside fluorescent rocks composed of fluorite, calcite and pyrite. The resultant growth was then measured following exposure to UV radiation, with the use of optical density and measurement of chlorophyll concentration. Results show that the microorganisms were shielded from harmful UV in these semi-transparent rocks, while at the same time benefiting from the fact that the minerals converted UV to visible light; this have been shown by a statistically significant increase in their growth, which although lower than when the cells were incubated in sunlight, was significantly higher than in controls incubated in the dark. Copyright © 2015 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.

  11. Abundance and Night Hourly Dispersal of the Vesicating Beetles of the Genus Paederus (Coleoptera: Staphylinidae) Attracted to Fluorescent, Incandescent, and Black Light Sources in the Brazilian Savanna.

    Science.gov (United States)

    Lima, D C B; Costa, A A V; Silva, F S

    2015-01-01

    Paederus beetles are cosmopolitan medically important insects that cause dermatitis linearis to humans. In Brazil, despite the medical importance of these beetles, no studies focusing directly on the abundance and ecological features of harmful species exist. Therefore, this study aims at determining the abundance and the nocturnal hourly dispersal of Paederus species attracted to fluorescent, incandescent, and black light sources in the Brazilian savanna. Paederus species were captured from May to September for three consecutive years, between 2011 and 2013. The specimens were caught hourly, from 1800 to 0600 hours. Paederus beetles were attracted to incandescent, fluorescent, and black light lamps as light sources. A total of 959 individuals of five species were collected. The collected species were Paederus protensus Sharp (59.85%), Paederus columbinus Laporte de Castelnau (29.20%), Paederus mutans Sharp (7.09%), Paederus brasiliensis Erichson (3.34%), and Paederus ferus Erichson (0.52%). The black light was the most attractive source, and the darkest collecting point was the most representative for the number of individuals. The lowest catches were captured at full moon, and the highest catches were between 2200 and 0100 hours. Future investigations are needed to better understand the role of night temperature and soil humidity affecting the seasonal growth of Paederus beetle populations of northeastern Brazil. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  12. Effect of fluorescent light on selected antioxidant compounds and antioxidant activity during storage of fresh-cut carambola (averrhoa carambola l.) fruit

    International Nuclear Information System (INIS)

    Zainudin, M.A.M.; Dek, M.S.P.; Anwar, F.

    2012-01-01

    The effects of fluorescent light on the availability of selected antioxidant compounds and antioxidant activity during storage of fresh-cut carambola (Averrhoa carambola L.) fruit were investigated. The samples of fresh-cut fruit, stored at 5 +- 1 deg. C, were exposed to fluorescent light (157 Lux) for 12 days. Total phenolic compounds (TPC) and ascorbic acid (AA) content of the processed fruit were analyzed using Folin-Ciocalteu reagent and high performance liquid chromatography (HPLC), respectively. Antioxidant activity of the fruit was assessed following 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity and ferric-reducing antioxidant power (FRAP) assays. The results of the present study showed that light exposure did not exert any significant effect, while storage period significantly reduced the AA content of the fruits tested. On the other hand, with few exceptions, there were notable random changes, recorded in the TPC and antioxidant activity of the fruit as function of storage period and light exposure. (author)

  13. The Role of Light-Induced Fluorescence in the Treatment of Smooth Surface Carious Lesions with Icon Infiltration and the Results After 1 Year

    Directory of Open Access Journals (Sweden)

    Kabaktchieva R.

    2014-12-01

    Full Text Available Caries infiltration is a novel technique that brings out immediate esthetic improvement in the opacity of the white spot lesions. Light-induced fluorescence method is a modern caries diagnostic method. In this study SoproLife camera (Acteon, France was applied for diagnosing and follow-up of the results. The aims of this in vivo study are to test the role of light-induced fluorescence method (SoploLife camera in the diagnosis of non-cavitated smooth surfaces carious lesions (ICDAS codes 1 and 2 of primary and permanent teeth and in the follow-up period immediately after application, 6 months and 1 year after applying ICON material (DMG. Teeth: n = 90; primary teeth: 6 kids; n = 40 teeth; permanent teeth: 6 patients; n = 50 teeth. Visual examination by ICDAS without probe, dry for 10 s with 3-in-1 syringe using lightening; SoproLife camera (450 nm, digital photos. LIF method applied with SoproLife camera (Diagnostic mode with day light and blue light is more accurate than visual examination only when applied for single tooth diagnose. Moreover, LIF method for single tooth is more accurate in following up the effect of non-operative treatment of smooth surfaces lesions than using digital images. ICON is a material that stops the progression of non-cavitated smooth surfaces carious lesions in both primary and permanent teeth and make the aesthetic result better up to 1 year following the procedure.

  14. Fluorescence excitation spectroscopic imaging with a tunable light source and dimensionality reduction using FR-IsoMap

    Science.gov (United States)

    Koh, Kevin; Wood, Tobias; Zhang, Hao; Lekadir, Karim; Elson, Daniel; Yang, Guang-Zhong

    2008-02-01

    This paper presents an optical system for performing excitation resolved imaging of fluorescent dyes, tissue phantoms and ex vivo tissues. The excitation source was a supercontinuum generated in a highly nonlinear fibre, spectrally filtered using dispersive optics and a movable slit or digital micromirror device. This allowed excitation with multiple spectra, which may be chosen to optimize the fluorescence yield from a particular fluorophore, or to maximize discrimination between multiple labels. As an initial validation, the analysis of fluorescent dye solutions embedded in collagen gels in the presence of scatterers and absorbers by using fixed reference isomap (FR-IsoMap) is presented, followed by initial evaluation with human lung bronchial tissue. The proposed system has potential applications for in vivo fluorescence endoscopy of cancerous tissues through excitation spectral selection and analysis with FR-IsoMap.

  15. The erosive potential of commercially available mouthrinses on enamel as measured by Quantitative Light-induced Fluorescence (QLF).

    Science.gov (United States)

    Pretty, I A; Edgar, W M; Higham, S M

    2003-07-01

    Longitudinal in vitro. Previously extracted, caries free, human premolars were selected and prepared by gentle pumicing and coating in an acid-resistant nail-varnish save for an exposed enamel window on the buccal surface. Each was assigned to one of eight groups (six per group, 10 in positive control); positive control (citric acid, pH 2.7, F(-) 0 ppm), negative control (pH 7.0, F(-) 0 ppm) Listerine (pH 3.87, F(-) 0.021 ppm), Tesco Value (pH 6.05, F(-) 289.00 ppm), Tesco Total Care (pH 6.20, F(-) 313.84 ppm), Sainsbury's (pH 6.15, F(-) 365.75 ppm), Sensodyne (pH 6.12, F(-) 285.30 ppm) and Corsodyl (pH 5.65, F(-) 0 ppm). The titratable acid values (TAV) for each rinse were established using volume (ml) of 0.1 M NaOH to achieve pH 7. Fluoride values were obtained by ion selective electrode. The solutions were kept at 37 degrees C and gently agitated. Teeth were removed at hourly intervals for 15 h, air-dried and subjected to Quantitative Light-induced Fluorescence (QLF) examination by a blinded examiner and DeltaQ values recorded. At the conclusion of the study each of the positive control teeth and one from each other group were sectioned through the eroded lesion, ground and polished to 100 micrometers and subjected to transverse microradiography and DeltaZ recorded for validation. TAVs were: Listerine 2.45 L > Sainsbury's 0.35 ml >Tesco Total Care 0.14 ml > Tesco Value 0.08 ml > Corsodyl 0.10 ml >Sensodyne 0.9 ml. DeltaQ increased over time for the positive control, (0 h 0.2, 10 h 95.2, 15 h 152.3). Negative controls remained stable. The increase in DeltaQ for each rinse after 15 h was Listerine (9.3(+/-7.2)), Corsodyl (1.5(+/-1.2)), Tesco Value (1.8(+/-1.2)), Tesco Total Care (1.4(+/-1.1)), Sainsbury's (3.4(+/-2.2)), Sensodyne (0.9(+/-1.6)). TMR confirmed the presence/absence of erosive lesions. QLF effectively monitored erosion in the positive controls and lack of erosion in the NC. Only one mouthrinse (Listerine) caused any erosion compared to the negative

  16. Plasmonic enhancement of cyanine dyes for near-infrared light-triggered photodynamic/photothermal therapy and fluorescent imaging

    Science.gov (United States)

    Lu, Mindan; Kang, Ning; Chen, Chuan; Yang, Liuqing; Li, Yang; Hong, Minghui; Luo, Xiangang; Ren, Lei; Wang, Xiumin

    2017-11-01

    Near-infrared (NIR) triggered cyanine dyes have attracted considerable attention in multimodal tumor theranostics. However, NIR cyanine dyes used in tumor treatment often suffer from low fluorescence intensity and weak singlet oxygen generation efficiency, resulting in inadequate diagnostic and therapy efficacy for tumors. It is still a great challenge to improve both the photodynamic therapy (PDT) and fluorescent imaging (FLI) efficacy of cyanine dyes in tumor applications. Herein, a novel multifunctional nanoagent AuNRs@SiO2-IR795 was developed to realize the integrated photothermal/photodynamic therapy (PTT/PDT) and FLI at a very low dosage of IR795 (0.4 μM) based on metal-enhanced fluorescence (MEF) effects. In our design, both the fluorescence intensity and reactive oxygen species of AuNRs@SiO2-IR795 nanocomposites were significantly enhanced up to 51.7 and 6.3 folds compared with free IR795, owing to the localized surface plasmon resonance band of AuNRs overlapping with the absorption or fluorescence emission band of the IR795 dye. Under NIR laser irradiation, the cancer cell inhibition efficiency in vitro with synergetic PDT/PTT was up to 82.3%, compared with 10.3% for free IR795. Moreover, the enhanced fluorescence intensity of our designed nanocomposites was helpful to track their behavior in tumor cells. Therefore, our designed nanoagents highlight the applications of multimodal diagnostics and therapy in tumors based on MEF.

  17. On the relationship between the non-photochemical quenching of the chlorophyll fluorescence and the Photosystem II light harvesting efficiency. A repetitive flash fluorescence induction study

    Czech Academy of Sciences Publication Activity Database

    Koblížek, Michal; Kaftan, David; Nedbal, Ladislav

    2001-01-01

    Roč. 68, č. 2 (2001), s. 141-152 ISSN 0166-8595 Institutional research plan: CEZ:AV0Z6087904 Keywords : Light adaptation * non-photochemical quenching * Phaeodactylum tricornutum Subject RIV: BO - Biophysics Impact factor: 1.739, year: 2001

  18. The Retrofit Puzzle Extended: Optimal Fleet Owner Behavior over Multiple Time Periods

    Science.gov (United States)

    2009-08-04

    In "The Retrofit Puzzle: Optimal Fleet Owner Behavior in the Context of Diesel Retrofit Incentive Programs" (1) an integer program was developed to model profit-maximizing diesel fleet owner behavior when selecting pollution reduction retrofits. Flee...

  19. RETROFITTING DENIZ PALACE HISTORIC BUILDING FOR REUSING

    Directory of Open Access Journals (Sweden)

    Ezgi KORKMAZ

    2013-01-01

    Full Text Available There is a big stock of historic structures in Turkey. Many of those structures continue to serve by changing their functions which brings new loads to the building. During their long life, historic structures have experienced many actions occurred over long periods of time and endured long term deteriorating effects and earthquake loads, besides these effects changing of the function affects the load bearing capacity of the building. This study is focused on restoration and retrofitting of historic multi storey masonry building named Deniz Palace Building which is located in Istanbul where the whole city is seismic prone area itself. Earthquake resistance is the main purpose of decision for retrofitting and strengthening of the building. In this sense walls are sheathed by concrete, floors are strengthened by steel I profiles and concrete, and foundations are reconstructed. During the restoration, interventions are made based on prevention of historic values. Static calculations are done particularly. For this purpose, foundation retrofitting is made primarily and some critical walls are sheathed by concrete. Fire protection system is designed and according to the evacuation plan some additions such as elevator shaft are constructed.

  20. Low-watt lamps seen bad in many retrofits: said to burn out pre-1978 standard ballasts

    Energy Technology Data Exchange (ETDEWEB)

    Cohn, L.

    1982-06-21

    The standard ballasts used in early energy-efficient fluorescent lamps are vulnerable to premature burnout. Retrofitting expensive new ballasts will eliminate any savings from the lamps. GTE/Sylvania, Norelco, and Westinghouse acknowledged the problem, while General Electric had no comment. Ballasts made in the last four years have been made compatible with energy-efficient lamps. The vendors claim it is the ballast manufacturers' responsibility to inform users that their product risks early burnout. (DCK)

  1. Ultraviolet radiation levels associated with the use of fluorescent general lighting, UV-A and UV-B lamps in the workplace and home

    International Nuclear Information System (INIS)

    Whillock, M.; Clark, I.E.; McKinlay, A.F.; Todd, C.D.; Mundy, S.J.

    1988-09-01

    A detailed programme of measurements was undertaken by NRPB to determine the ultraviolet irradiance levels likely to be encountered in the workplace and in the home, where fluorescent lighting is used. Assessments have been made of the possible potential risk of the induction of acute effects (photokeratitis, erythema) and of inducing malignant melanoma and non-malignant melanoma skin cancers resulting from exposure to commonly used fluorescent lamps. The optical absorption properties of materials commonly used in diffusers and controllers in commercial and domestic lighting units were also measured. Irradiance data, both weighted (for biological effectiveness) and unweighted, for various lamp types are presented in the report, together with some typical spectral output distributions. The results show that at commonly used illumination levels the UVR emissions from general and special fluorescent lamps presented neither an acute nor a significant chronic hazard. High UV-B emission levels were measured from 'UV-B' lamps used in this study, and exposure to these lamps would result in acute injury within a short time. Great care should be taken in the use of these lamps, and advice should be provided to workers to ensure safe working conditions and procedures. (author)

  2. A design of fluorescent fiber solar concentrator (FFSC and outdoor testing for remote indoor day lighting and power producing evaluation for building integration

    Directory of Open Access Journals (Sweden)

    Wang Chen

    2008-12-01

    Full Text Available Different from the conventional luminescent solar concentrator (LSC, a 1200mmx1200m m solar concentrator consisting of 150 pieces of three-color 1m long, 2mm diameter fluorescent fibers (FFSC has been designed and mounted on a University building roof and the concentrated light is transported to a remote dark room through 10m long, 201m diameter clear optical fibers. Outdoor testing for remote indoor day lighting and power producing evaluation has been conducted. A 31-day monitored data from 24 May2ooS to 23 june200S has been presented and the results reveal that even though FFSC is not practical yet to replace the conventional BIPV approach for power producing, it has a pleasant potential in remote indoor day lighting {or large amount application in building integration.

  3. Ready to Retrofit: The Process of Project Team Selection, Building Benchmarking, and Financing Commercial Building Energy Retrofit Projects

    Energy Technology Data Exchange (ETDEWEB)

    Sanders, Mark D. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Parrish, Kristen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Mathew, Paul [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2012-05-01

    This guide presents a process for three key activities for the building owner in preparing to retrofit existing commercial buildings: selecting project teams, benchmarking the existing building, and financing the retrofit work. Although there are other essential steps in the retrofit process, the three activities presented in this guide are the critical elements where the building owner has the greatest influence on the outcome of the project.

  4. A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.

    Science.gov (United States)

    Pompidor, Guillaume; Dworkowski, Florian S N; Thominet, Vincent; Schulze-Briese, Clemens; Fuchs, Martin R

    2013-09-01

    The combination of X-ray diffraction experiments with optical methods such as Raman, UV/Vis absorption and fluorescence spectroscopy greatly enhances and complements the specificity of the obtained information. The upgraded version of the in situ on-axis micro-spectrophotometer, MS2, at the macromolecular crystallography beamline X10SA of the Swiss Light Source is presented. The instrument newly supports Raman and resonance Raman spectroscopy, in addition to the previously available UV/Vis absorption and fluorescence modes. With the recent upgrades of the spectral bandwidth, instrument stability, detection efficiency and control software, the application range of the instrument and its ease of operation were greatly improved. Its on-axis geometry with collinear X-ray and optical axes to ensure optimal control of the overlap of sample volumes probed by each technique is still unique amongst comparable facilities worldwide and the instrument has now been in general user operation for over two years.

  5. Retrofitting Garden-Style Apartments in Brooklyn, New York

    Energy Technology Data Exchange (ETDEWEB)

    Nolen, Heather [Steven Winter Associates, Inc., Norwalk, CT (United States); Maxwell, Sean [Steven Winter Associates, Inc., Norwalk, CT (United States); Neri, Robin [Steven Winter Associates, Inc., Norwalk, CT (United States); Grab, Joanna [Steven Winter Associates, Inc., Norwalk, CT (United States)

    2016-02-01

    This research effort sought to achieve a solution package that yields energy savings greater than 30% over the pre-existing conditions in a minimally intrusive multifamily retrofit project. The Consortium for Advanced Residential Buildings (CARB) partnered with L+M Development Partners, Inc. on a Mitchell-Lama Housing Program project, Marcus Garvey Village, in Brooklyn, NY (Climate Zone 4A). The Mitchell-Lama Housing Program is a form of housing subsidy in the state of New York that provides affordable rental and cooperative housing to moderate- and middle-income families. Marcus Garvey Village was founded in 1975 and contains 625 residential units (ranging from studios to 5-bedroom units) in thirty-two 4-story garden-style apartment structures built with concrete and faced in light brown brick. The single largest challenge for implementation of energy conservation measures at Marcus Garvey was working within occupied spaces. Measures are being implemented in phases to minimize disruption. As of August 2015, the retrofit work is over 50% complete. The wall insulation, sealing of the through wall AC vent, and installation of new oil-filled electric baseboards with advanced controls are conducted at one time, limiting disruption to the living room space. In a similar fashion, the kitchen work is done, then the bathroom. The final selection of energy conservation measures is projected to save 26.5% in source energy with a cost just under $3.7 million and utility bill savings of nearly $480,000 (of an average $1.8 million annual utility cost for the development).

  6. Versatile spectrophotometer for photosynthesis (light-induced changes in absorbance and fluorescence yield, circular and linear dichroism) and other biophysical measurements

    Science.gov (United States)

    Ke, Bacon; Breeze, Robert H.; Dolan, Edward; Vore, Duane

    1985-01-01

    The construction of a versatile, single-beam spectrophotometer expanded from a previously reported circular dichrometer utilizing an elasto-optic modulator (EOM) for producing circular polarization, is described. The new spectrophotometer is capable of measuring circular dichroism as well as linear dichroism and light-induced changes in absorbance and fluorescence yield, the latter being two of the most commonly measured properties in photosynthesis research. The use of an EOM, along with a phase-sensitive detector, affords high sensitivity and at the same time, in conjuction with a mechanical phosphoroscopic device, a high degree of immunity from artifacts in studies employing actinic light. A description is given of the generation and use of timing signals essential for control and signal processing. Its performance is illustrated by examples drawn from light-induced absorbance and fluorescence-yield changes associated with photosynthetic electron transport and changes in redox state of electron carriers. This photometer, with very little modification, may also be used to perform a host of other types of measurements.

  7. Building exterior retrofit and its impact on energy performance - a case study

    Energy Technology Data Exchange (ETDEWEB)

    Trovato, Nick [Read Jones Christoffersen Ltd (Canada)

    2011-07-01

    The building envelope plays a vital role in energy performance and the comfort of the building's occupants. The office building of Read Jones Christofferesen Ltd. (RJC) had excessive condensation problems with water and air leakages resulting in high-energy consumption. Hence, it required a building envelope retrofit. This paper presents a description of the existing building, improvements made to the building envelope and the mechanical system and the integration of the two systems and how this impacted the energy performance of the building. The owner had certain requirements for the retrofit, such as optimum climate control, wide window opening space, and maximum lighting. Exterior upgrades included, among others, stopping the air and water leakage, improved thermal resistance and reduced vapor diffusion. The renovation resulted in reduced energy consumption, a more comfortable working environment, better mechanical system control, and around 65% in gas consumption and mechanical cost savings.

  8. On the polyphasic quenching kinetics of chlorophyll a fluorescence in algae after light pulses of variable length

    Czech Academy of Sciences Publication Activity Database

    Vredenberg, W.; Prášil, Ondřej

    2013-01-01

    Roč. 117, 1-3 (2013), s. 321-337 ISSN 0166-8595 R&D Projects: GA MŠk(CZ) ED2.1.00/03.0110 Institutional support: RVO:61388971 Keywords : Chlorophyll a fluorescence * Photosystem II * Quenching kinetics Subject RIV: EE - Microbiology, Virology Impact factor: 3.185, year: 2013

  9. Detection of Silver Nanoparticles in Cells by Flow Cytometry Using Light Scattering and Far-red Fluorescence

    Science.gov (United States)

    The cellular uptake of different sized silver nanoparticles (l0 nm, 50 nm, and 75nm) coated with polyvinylpyrrolidone (PVP) or citrate in ARPE-19 cells following 24 hour incubation was detected by side scatter through the use of a flow cytometer. A large far red fluorescence sign...

  10. Arkansas Solar Retrofit Guide. Greenhouses, Air Heaters and Water Heaters.

    Science.gov (United States)

    Skiles, Albert; Rose, Mary Jo

    Solar retrofits are devices of structures designed to be attached to existing buildings to augment their existing heating sources with solar energy. An investigation of how solar retrofits should be designed to suit the climate and resources of Arkansas is the subject of this report. Following an introduction (section 1), section 2 focuses on…

  11. Multicriteria decision model for retrofitting existing buildings

    Science.gov (United States)

    Bostenaru Dan, B.

    2003-04-01

    In this paper a model to decide which buildings from an urban area should be retrofitted is presented. The model has been cast into existing ones by choosing the decision rule, criterion weighting and decision support system types most suitable for the spatial problem of reducing earthquake risk in urban areas, considering existing spatial multiatributive and multiobjective decision methods and especially collaborative issues. Due to the participative character of the group decision problem "retrofitting existing buildings" the decision making model is based on interactivity. Buildings have been modeled following the criteria of spatial decision support systems. This includes identifying the corresponding spatial elements of buildings according to the information needs of actors from different sphaeres like architects, construction engineers and economists. The decision model aims to facilitate collaboration between this actors. The way of setting priorities interactivelly will be shown, by detailing the two phases: judgemental and computational, in this case site analysis, collection and evaluation of the unmodified data and converting survey data to information with computational methods using additional expert support. Buildings have been divided into spatial elements which are characteristic for the survey, present typical damages in case of an earthquake and are decisive for a better seismic behaviour in case of retrofitting. The paper describes the architectural and engineering characteristics as well as the structural damage for constuctions of different building ages on the example of building types in Bucharest, Romania in compressible and interdependent charts, based on field observation, reports from the 1977 earthquake and detailed studies made by the author together with a local engineer for the EERI Web Housing Encyclopedia. On this base criteria for setting priorities flow into the expert information contained in the system.

  12. Measure Guideline: Hybrid Foundation Insulation Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, K.; Lstiburek, J.

    2012-05-01

    This measure guideline provides recommendations for designs and variations for retrofit hybrid assemblies in improving interior foundation insulation and water management of basements. Variations include closed cell spray foam (ccSPF) with membrane waterproofing or air gap membrane drainage layers, rigid board foam insulation at flat walls (cast concrete or CMU block), a 'partial drainage' detail making use of the bulk water drainage that occurs through the field of a rubble stone wall, and non-drained spray foam assemblies (including slab insulation).

  13. Measure Guideline. Hybrid Foundation Insulation Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, K. [Building Science Corporation, Somerville, MA (United States); Lstiburek, J. [Building Science Corporation, Somerville, MA (United States)

    2012-05-01

    This measure guideline provides recommendations for designs and variations for retrofit hybrid assemblies in improving interior foundation insulation and water management of basements. Variations include closed cell spray foam (ccSPF) with membrane waterproofing or air gap membrane drainage layers, rigid board foam insulation at flat walls (cast concrete or CMU block), a “partial drainage” detail making use of the bulk water drainage that occurs through the field of a rubble stone wall, and non-drained spray foam assemblies (including slab insulation).

  14. Crowne Plaza Renovation Retrofit Case Study

    Energy Technology Data Exchange (ETDEWEB)

    none,

    2013-03-01

    InterContinental Hotels Group (IHG) and its franchise partner B.F. Saul Company Hospitality Group (B.F. Saul Co.) partnered with the Department of Energy (DOE) to develop and implement solutions to retrofit existing buildings to reduce energy consumption by at least 30% versus requirements set by Standard 90.1-2004 of the American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE), the American National Standards Institute (ANSI), and the Illuminating Engineering Society of North America (IESNA) as part of DOE’s Commercial Building Partnerships (CBP) Program.

  15. Retrofitting compressor engines to reduce emissions

    International Nuclear Information System (INIS)

    Collison, Melanie

    2011-01-01

    Cenovus Energy Inc. is upgrading its natural gas compression facilities at 37 sites it operates in Alberta. The project itself consists of a retrofit of the natural-fas fired engines that power the compressors that fill its natural gas sales pipe-line. Piping to capture fugitive natural gas will also be installed. These emissions will be used as fuel. The efficiency rating of such engine will be the same as a new fuel-injected engine. One of the challenge in the design of the parts of these engines ss to to ensure the least possible downtime to minimize production losses.

  16. Indoor climate in renovated and energy retrofitted social housing

    DEFF Research Database (Denmark)

    Knudsen, Henrik Nellemose; Jensen, Ole Michael

    2016-01-01

    The need for energy retrofitting of the Danish single-family houses is massive, especially for the high proportion of single-family houses built in the 1960s and 1970s. But even though the potential benefits are many, only few families embark on a major energy retrofit. There may be many reasons...... for this. An obvious one may be limited knowledge of non-energy benefits, e.g. in relation to the indoor climate. The objective of this study was to explain this limited effort to save energy by identifying barriers and incentives among house owners in relation to energy retrofitting of one’s own house....... Moreover, it was investigated among house owners, who had carried out energy retrofitting, whether a number of factors, including the perceived indoor climate, became better or worse after retrofitting. A questionnaire survey was carried out among 1,990 house owners in a municipality north of Copenhagen...

  17. Binding investigation on the interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin by resonance light-scattering (RLS) technique and fluorescence spectroscopy.

    Science.gov (United States)

    Li, Yuesheng; Zhang, Yue; Sun, Shaofa; Zhang, Aiqing; Liu, Yi

    2013-11-05

    The interaction between Methylene Blue (MB)/TiO2 nanocomposites and bovine serum albumin (BSA) was investigated by resonance light scattering (RLS), fluorescence, three-dimension spectra and UV-vis absorbance spectroscopy. Several factors which may influence the RLS intensity were also investigated before characterizing MB/TiO2-BSA complex. It was proved that the mechanism of MB/TiO2 nanocomposites binding to BSA was mainly a result of the formation of MB/TiO2-BSA complex. The binding constant of MB/TiO2-BSA is 0.762 × 10(-5) L mol(-1) at 298K. By calculating the binding constant at different temperature, the thermodynamic parameters ΔH, ΔG, and ΔS can be observed and deduced that the hydrophobic interactions played an important role to stabilize the complex. The distance r (3.73 nm) between donor (BSA) and acceptor (MB/TiO2) was obtained according to fluorescence resonance energy transfer (FRET). The binding site for MB/TiO2 on BSA was mainly located in sub-domain IIA. The UV-vis absorbance, circular dichroism and three dimension fluorescence have also been used to investigate the effect of MB/TiO2 on the conformation of BSA. Copyright © 2013 Elsevier B.V. All rights reserved.

  18. FLUORESCENCE EMISSION SPECTRA OF MARINE AND BRACKISH-WATER ECOTYPES OF FUCUS VESICULOSUS AND FUCUS RADICANS (PHAEOPHYCEAE) REVEAL DIFFERENCES IN LIGHT-HARVESTING APPARATUS(1).

    Science.gov (United States)

    Maria Gylle, Anna; Rantamäki, Susanne; Ekelund, Nils G A; Tyystjärvi, Esa

    2011-02-01

    The Bothnian Sea in the northerly part of the Baltic Sea is a geologically recent brackish-water environment, and rapid speciation is occurring in the algal community of the Bothnian Sea. We measured low-temperature fluorescence emission spectra from the Bothnian Sea and the Norwegian Sea ecotypes of Fucus vesiculosus L., a marine macroalga widespread in the Bothnian Sea. Powdered, frozen thallus was used to obtain undistorted emission spectra. The spectra were compared with spectra measured from the newly identified species Fucus radicans Bergström et L. Kautsky, which is a close relative of F. vesiculosus and endemic to the Bothnian Sea. The spectrum of variable fluorescence was used to identify fluorescence peaks originating in PSI and PSII in this chl c-containing alga. The spectra revealed much higher PSII emission, compared to PSI emission, in the Bothnian Sea ecotype of F. vesiculosus than in F. radicans or in the Norwegian Sea ecotype of F. vesiculosus. The results suggest that more light-harvesting chl a/c proteins serve PSII in the Bothnian Sea ecotype of F. vesiculosus than in the two other algal strains. Treatment of the Bothnian Sea ecotype of F. vesiculosus in high salinity (10, 20, and 35 practical salinity units) for 1 week did not lead to spectral changes, indicating that the measured features of the Bothnian Sea F. vesiculosus are stable and not simply a direct result of exposure to low salinity. © 2010 Phycological Society of America.

  19. X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

    Directory of Open Access Journals (Sweden)

    Christopher D. M. Hutchison

    2017-09-01

    Full Text Available The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on state at 1.9 Angstrom resolution and the trans (off state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.

  20. An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light.

    Science.gov (United States)

    Li, Chun-Xia; Xu, Zhi-Gang; Dong, Rui-Qi; Chang, Sheng-Xin; Wang, Lian-Zhen; Khalil-Ur-Rehman, Muhammad; Tao, Jian-Min

    2017-01-01

    Using an RNA sequencing (RNA-seq) approach, we analyzed the differentially expressed genes (DEGs) and physiological behaviors of "Manicure Finger" grape plantlets grown in vitro under white, blue, green, and red light. A total of 670, 1601, and 746 DEGs were identified in plants exposed to blue, green, and red light, respectively, compared to the control (white light). By comparing the gene expression patterns with the growth and physiological responses of the grape plantlets, we were able to link the responses of the plants to light of different spectral wavelengths and the expression of particular sets of genes. Exposure to red and green light primarily triggered responses associated with the shade-avoidance syndrome (SAS), such as enhanced elongation of stems, reduced investment in leaf growth, and decreased chlorophyll levels accompanied by the expression of genes encoding histone H3, auxin repressed protein, xyloglucan endotransglycosylase/hydrolase, the ELIP protein, and microtubule proteins. Furthermore, specific light treatments were associated with the expression of a large number of genes, including those involved in the glucan metabolic pathway and the starch and sucrose metabolic pathways; these genes were up/down-regulated in ways that may explain the increase in the starch, sucrose, and total sugar contents in the plants. Moreover, the enhanced root growth and up-regulation of the expression of defense genes accompanied with SAS after exposure to red and green light may be related to the addition of 30 g/L sucrose to the culture medium of plantlets grown in vitro . In contrast, blue light induced the up-regulation of genes related to microtubules, serine carboxypeptidase, chlorophyll synthesis, and sugar degradation and the down-regulation of auxin-repressed protein as well as a large number of resistance-related genes that may promote leaf growth, improve chlorophyll synthesis and chloroplast development, increase the ratio of chlorophyll a (chla

  1. DEEP: A Database of Energy Efficiency Performance to Accelerate Energy Retrofitting of Commercial Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Hoon Lee, Sang; Hong, Tianzhen; Sawaya, Geof; Chen, Yixing; Piette, Mary Ann

    2015-05-01

    The paper presents a method and process to establish a database of energy efficiency performance (DEEP) to enable quick and accurate assessment of energy retrofit of commercial buildings. DEEP was compiled from results of about 35 million EnergyPlus simulations. DEEP provides energy savings for screening and evaluation of retrofit measures targeting the small and medium-sized office and retail buildings in California. The prototype building models are developed for a comprehensive assessment of building energy performance based on DOE commercial reference buildings and the California DEER prototype buildings. The prototype buildings represent seven building types across six vintages of constructions and 16 California climate zones. DEEP uses these prototypes to evaluate energy performance of about 100 energy conservation measures covering envelope, lighting, heating, ventilation, air-conditioning, plug-loads, and domestic hot water. DEEP consists the energy simulation results for individual retrofit measures as well as packages of measures to consider interactive effects between multiple measures. The large scale EnergyPlus simulations are being conducted on the super computers at the National Energy Research Scientific Computing Center of Lawrence Berkeley National Laboratory. The pre-simulation database is a part of an on-going project to develop a web-based retrofit toolkit for small and medium-sized commercial buildings in California, which provides real-time energy retrofit feedback by querying DEEP with recommended measures, estimated energy savings and financial payback period based on users’ decision criteria of maximizing energy savings, energy cost savings, carbon reduction, or payback of investment. The pre-simulated database and associated comprehensive measure analysis enhances the ability to performance assessments of retrofits to reduce energy use for small and medium buildings and business owners who typically do not have resources to conduct

  2. A Conjugated Polyelectrolyte with Pendant High Dense Short-Alkyl-Chain-Bridged Cationic Ions: Analyte-Induced Light-Up and Label-Free Fluorescent Sensing of Tumor Markers

    Directory of Open Access Journals (Sweden)

    Nina Fu

    2017-06-01

    Full Text Available A novel cationic water-soluble conjugated polyelectrolyte (CPE of polyfluorene that contains 15% fraction of 2,1,3-benzothiadiazole (BT units (PFC3NBT has been obtained. PFC3NBT demonstrates intramolecular energy transfer from the fluorene segments to BT sites when negatively charged species (SDS or DNAs are added, following by a shift in emission color from blue to green, has been developed. The high density of positive charges and pendent short alkyl chains of N-propyltrimethylammoniums endow PFC3NBT with high solubility and high fluorescence quantum efficiency of 33.6% in water. The fluorescence emission properties were investigated in the presence of adverse buffer solutions, different surfactants and DNA strands. Interesting fluorescence emission quenching at short wavelength and fluorescence resonance energy transfer (FRET induced light-on at BT sites were observed and discussed in detail. Very different from previous reports, the fluorescence emission spectra transition happens with an enhancement of integrated fluorescent intensity. The analytes induced a light-up sensing system was studied with a PFC3NBT/SDS complex mode and confirmed with DNA/DNA-FAM sensing systems. More exciting preliminary results on label-free sensing of tumor markers were also reported by investigating the unique fluorescence response to 11 kinds of proteins. These results provide a new insight view for designing CPEs with light-up and label-free features for biomolecular sensing.

  3. Green-lighting green fluorescent protein: faster and more efficient folding by eliminating a cis-trans peptide isomerization event.

    Science.gov (United States)

    Rosenman, David J; Huang, Yao-ming; Xia, Ke; Fraser, Keith; Jones, Victoria E; Lamberson, Colleen M; Van Roey, Patrick; Colón, Wilfredo; Bystroff, Christopher

    2014-04-01

    Wild-type green fluorescent protein (GFP) folds on a time scale of minutes. The slow step in folding is a cis-trans peptide bond isomerization. The only conserved cis-peptide bond in the native GFP structure, at P89, was remodeled by the insertion of two residues, followed by iterative energy minimization and side chain design. The engineered GFP was synthesized and found to fold faster and more efficiently than its template protein, recovering 50% more of its fluorescence upon refolding. The slow phase of folding is faster and smaller in amplitude, and hysteresis in refolding has been eliminated. The elimination of a previously reported kinetically trapped state in refolding suggests that X-P89 is trans in the trapped state. A 2.55 Å resolution crystal structure revealed that the new variant contains only trans-peptide bonds, as designed. This is the first instance of a computationally remodeled fluorescent protein that folds faster and more efficiently than wild type. © 2014 The Protein Society.

  4. Reaction dynamics during pulsed light activation of ATX-S10 Na(II)-sensitized cell cultures: analysis based on fluorescence-oxygen diagram

    Science.gov (United States)

    Kawauchi, Satoko; Sato, Shunichi; Morimoto, Yuji; Kikuchi, Makoto

    2005-04-01

    To elucidate the mechanism of photosensitization with pulsed light excitation, we previously introduced fluorescence-oxygen diagram that shows the correlation between photochemical oxygen consumption and photobleaching during a treatment (Kawauchi et al., Photochem. Photobiol., 80, 216-223, 2004). In pulsed photodynamic treatment of A549 cells with ATX-S10"Na(II), the diagrams for treatments at relatively high repetition rates of 10 and 30 Hz showed the complex behaviors of photochemical reaction; photobleaching initially occurred with oxygen consumption but it was switched to oxygen-independent photobleaching, which was followed by a secondary oxygen-consuming regime. In this study, fluorescence microscopy revealed that for treatments at 10 and 30 Hz, subcellular fluorescence distribution of ATX-S10×Na(II) changed drastically from the high-intensity spotty patterns showing lysosomal accumulation to the diffusive patterns within the cytosol during certain ranges of total light dose. These ranges were found to coincide with those in which oxygen-independent reaction appeared. These findings suggest that the sensitizer started to be redistributed from lysosomes to the cytosol during the oxygen-independent reaction regime. On the other hand, at 5 Hz, such reaction switching was not clearly seen during whole irradiation period in the diagram; this was consistent with the observation that sensitizer redistribution efficiently occurred even in the early phase of irradiation. The appearance of oxygen-independent reaction at the higher repetition rates may be caused by high local concentration of the sensitizer and the resultant low concentration of oxygen in the reaction sites due to the shorter pulse-to-pulse time intervals. In pulsed photodynamic treatment, pulse frequency is an important parameter that affects the intracellular kinetics of the sensitizer and hence the photochemical reaction dynamics.

  5. Skylight Application for Natural Lighting in a Building; the University of Tehran Branch of Melli Bank Case Study

    Directory of Open Access Journals (Sweden)

    Nasersharifi Yahya

    2014-07-01

    Full Text Available This study investigated the quality and potential lighting energy savings in a bank building retrofitting project in Tehran by using solar passive lighting. The retrofitting scenarios are based on OFFICE project findings. Despite the increase in occupancy and service quality of the bank after retrofitting, the peak electricity consumption significantly decreased (approximately 20%. The results showed that even the proper solar passive lighting in most of day hours had been enough to satisfy the existing building luminance before retrofitting due to the initial passive design of the building, however, by retrofitting and improving the lighting system using skylight and artificial lighting the quality of lighting increased meaningfully to cover the standards of lighting for precision activities required for banking.

  6. Light

    CERN Document Server

    Robertson, William C

    2003-01-01

    Why is left right and right left in the mirror? Baffled by the basics of reflection and refraction? Wondering just how the eye works? If you have trouble teaching concepts about light that you don t fully grasp yourself, get help from a book that s both scientifically accurate and entertaining with Light. By combining clear explanations, clever drawings, and activities that use easy-to-find materials, this book covers what science teachers and parents need to know to teach about light with confidence. It uses ray, wave, and particle models of light to explain the basics of reflection and refraction, optical instruments, polarization of light, and interference and diffraction. There s also an entire chapter on how the eye works. Each chapter ends with a Summary and Applications section that reinforces concepts with everyday examples. Whether you need a deeper understanding of how light bends or a good explanation of why the sky is blue, you ll find Light more illuminating and accessible than a college textbook...

  7. Air Distribution Retrofit Strategies for Affordable Housing

    Energy Technology Data Exchange (ETDEWEB)

    Dentz, Jordan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Conlin, Francis [National Renewable Energy Lab. (NREL), Golden, CO (United States); Holloway, Parker [National Renewable Energy Lab. (NREL), Golden, CO (United States); Podorson, David [National Renewable Energy Lab. (NREL), Golden, CO (United States); Varshney, Kapil [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-03-01

    In multifamily and attached buildings, traditional duct sealing methods are often impractical or costly and disruptive because of the difficulty in accessing leakage sites. In this project, two retrofit duct sealing techniques -- manually-applied sealants and injecting a spray sealant, were implemented in several low-rise multi-unit buildings. An analysis on the cost and performance of the two methods are presented. Each method was used in twenty housing units: approximately half of each group of units are single story and the remainder two-story. Results show that duct leakage to the outside was reduced by an average of 59% through the use of manual methods, and by 90% in the units where the injected spray sealant was used. It was found that 73% of the leakage reduction in homes that were treated with injected spray sealant was attributable to the manual sealing done at boots, returns and the air handler. The cost of manually-applying sealant ranged from $275 to $511 per unit and for the injected spray sealant the cost was $700 per unit. Modeling suggests a simple payback of 2.2 years for manual sealing and 4.7 years for the injected spray sealant system. Utility bills were collected for one year before and after the retrofits. Utility bill analysis shows 14% and 16% energy savings using injected spray sealant system and hand sealing procedure respectively in heating season whereas in cooling season, energy savings using injected spray sealant system and hand sealing were both 16%.

  8. Air Distribution Retrofit Strategies for Affordable Housing

    Energy Technology Data Exchange (ETDEWEB)

    Dentz, J. [Advanced Residential Integrated Energy Solutions (ARIES), New York, NY (United States); Conlin, F. [Advanced Residential Integrated Energy Solutions (ARIES), New York, NY (United States); Holloway, Parker [Advanced Residential Integrated Energy Solutions (ARIES), New York, NY (United States); Podorson, David [Advanced Residential Integrated Energy Solutions (ARIES), New York, NY (United States); Varshney, Kapil [Advanced Residential Integrated Energy Solutions (ARIES), New York, NY (United States)

    2014-03-01

    In multifamily and attached buildings, traditional duct sealing methods are often impractical or costly and disruptive because of the difficulty in accessing leakage sites. In this project, two retrofit duct sealing techniques, manually-applied sealants and injecting a spray sealant, were implemented in several low-rise multiunit buildings. An analysis on the cost and performance of the two methods are presented. Each method was used in twenty housing units: approximately half of each group of units are single story and the remainder are two story. Results show that duct leakage to the outside was reduced by an average of 59% through the use of manual methods, and by 90% in the units where the injected spray sealant was used. It was found that 73% of the leakage reduction in homes that were treated with injected spray sealant was attributable to the manual sealing done at boots, returns and the air handler. The cost of manually-applying sealant ranged from $275 to $511 per unit and for the injected spray sealant the cost was $700 per unit. Modeling suggests a simple payback of 2.2 years for manual sealing and 4.7 years for the injected spray sealant system. Utility bills were collected for one year before and after the retrofits. Utility bill analysis shows 14% and 16% energy savings using injected spray sealant system and hand sealing procedure respectively in heating season whereas in cooling season, energy savings using injected spray sealant system and hand sealing were both 16%.

  9. Light

    CERN Document Server

    Rivera, Andrea

    2017-01-01

    Light is all around us. Learn how it is used in art, technology, and engineering. Five easy-to-read chapters explain the science behind light, as well as its real-world applications. Vibrant, full-color photos, bolded glossary words, and a key stats section let readers zoom in even deeper. Aligned to Common Core Standards and correlated to state standards. Abdo Zoom is a division of ABDO.

  10. Factor Analysis on Criteria Affecting Lean Retrofit for Energy Efficient Initiatives in Higher Learning Institution Buildings

    Directory of Open Access Journals (Sweden)

    Abidin Nur IzieAdiana

    2017-01-01

    Full Text Available The expansion of Higher Learning Institution (HLI is a global concerns on energy demand due to campus act like a small city. Intensive mode of operation of a building is correlated to the energy utilization. Improvement in the current energy efficiency is crucial effort to minimize the environmental effect through minimisation of energy in operation by retrofitting and upgrade the existing building system or components to be more efficient. Basically, there are three recommended steps for the improvement known as lean initiatives, green technology and clean energy in response to becoming zero energy solutions for building. The deliberation of this paper is aimed to highlight the criteria affecting in retrofitting of existing building in HLI with lean initiatives in order to achieve energy efficiency and reduction of energy comsumption. Attention is devoted to reviewing the lean energy retrofitting initiatives criteria for daylighting (side lighting, daylighting (skylight and glazing. The questionnaire survey was employed and distributed to the architects who has an expertise in green building design. Factor analysis was adopted as a method of analysis by using Principal Component with Varimax Rotation. The result is presented through summarizing the sub-criteria according to its importance with a factor loading 0.50 and above. The result found that majority of the criteria developed achieved the significant factor loading value and in accordance with the protocal of analysis. In conclusion the results from analysis of this paper assists the stakeholders in assessing the significant criteria based on the desired lean energy retrofitting initiatives and also provides a huge contribution for future planning improvement in existing buildings to become an energy efficient building.

  11. What encourages local authorities to engage with energy performance contracting for retrofitting? Evidence from German municipalities

    International Nuclear Information System (INIS)

    Polzin, Friedemann; Flotow, Paschen von; Nolden, Colin

    2016-01-01

    Municipalities aiming at mitigating climate change by implementing new energy efficiency technologies face budgetary and capacity constraints. Outsourcing through energy service contracting could provide a solution. This paper reports results from a survey of 1298 municipalities concerning barriers to retrofitting public street lighting and the possible role of energy service contracting to overcome these barriers. Using a logistic regression analysis, the authors investigate determinants of opting for energy service contracts in the specific context of LED retrofits. Results point to an advantage of outsourcing in a financially and capacity-constrained environment, which corresponds with the main reasons for engaging in contracting: minimising investments and financial risks. However, municipalities often do not fully grasp the risks associated with retrofitting especially using a novel technology such as LED. In relation to that they underestimate the risk reduction potential of energy performance contracts (EPC). Previous experience with outsourcing increases the probability to engage in servitization although certain existing partnerships, particularly with utilities, prevent municipalities from considering energy performance contracts. Interestingly, engaging an energy consultant has a negative propensity to use energy service contracts, while pre-negotiated standardised contracts for energy performance contracts have a positive influence. - Highlights: •Barriers to retrofitting can be addressed through energy performance contracts (EPC). •Outsourcing has the potential to alleviate personnel and financial constraints. •Standardised contracts and previous experience with outsourcing facilitate use of EPC. •Engaging energy consultants has a negative influence on the willingness to adopt EPC. •Policy makers could support facilitators and standard contracts to boost EPC market.

  12. The effect of surface defects in early caries assessment using quantitative light-induced fluorescence (QLF) and micro-digital-photography (MDP).

    Science.gov (United States)

    Meharry, M R; Dawson, D; Wefel, J S; Harless, J D; Kummet, C M; Xiao, X

    2012-11-01

    The purpose of this study was to consider the impact of surface defects on quantitative light-induced fluorescence (QLF) and micro-digital-photography (MDP) measures, in relationship to lesion depth. Simulated enamel carious lesions were developed on 45 extracted human teeth. Images of each tooth were captured with both QLF and MDP. The teeth were sectioned and lesion depth was measured with polarized light microscopy (PLM). Pearson correlations were computed using data from the 27 lesions which did not have surface loss, and then separately based upon the 18 lesions which did display surface loss. MDP variables ΔR and ΔX measure reflected light, whereas QLF variables ΔF and ΔQ measure fluorescence. A strong correlation was identified between lesion depth and ΔF (r=-0.765, pMDP measures. QLF measures ΔF and ΔQ were strongly correlated with lesion depth in lab-simulated lesions with no surface loss, but not among lesions with surface defects. The two MDP-associated measures, ΔR and ΔX, could not be said to differ significantly when lesions with and without surface defects were compared with lesion depth. Because intact lesions can be remineralized, accurate assessment of their status is imperative for caries treatment. Dental caries is still widely prevalent today. We now know that with early stage detection, remineralization can be accomplished. Being able to identify dental caries in its reversible stage (before physical surface loss) is paramount for the clinician to be able to treat the disease non-invasively. Copyright © 2012 Elsevier Ltd. All rights reserved.

  13. Enhanced photocatalytic activity and characterization of magnetic Ag/BiOI/ZnFe2O4 composites for Hg0 removal under fluorescent light irradiation

    Science.gov (United States)

    Li, Chengwei; Zhang, Anchao; Zhang, Lixiang; Song, Jun; Su, Sheng; Sun, Zhijun; Xiang, Jun

    2018-03-01

    A series of magnetic Ag/BiOI/ZnFe2O4 hybrids synthesized via hydrothermal process, subsequent deposition-precipitation and photoreduction method were employed to remove elemental mercury (Hg0) under fluorescent light irradiation. The effects of Ag content, fluorescent light irradiation, reaction temperature, pH value, flue gas composition, anions and photocatalyst dosage on Hg0 removal were investigated in detail. The as-synthesized photocatalysts were characterized using N2 adsorption-desorption, XRD, SEM, TEM, HRTEM, XPS, VSM, DRS, ESR, PL and photocurrent response. The results showed that the ternary Ag/BiOI/ZnFe2O4 hybrids possessed enhanced visible-light-responsive photocatalytic performances for Hg0 removal. Ag/BiOI/ZnFe2O4 photocatalyst could be easily recovered from the reaction solution by an extra magnet and was stable in the process of Hg0 removal. Lower content of Ag was highly dispersed on the surface of BiOI/ZnFe2O4, while higher content of Ag would result in some aggregations and/or the blockages of micropore. In comparison to BiOI/ZnFe2O4, Ag deposited BiOI/ZnFe2O4 material showed lower recombination rate of electron-hole pairs. The superior Hg0 oxidation removal could correspond to good match of BiOI and ZnFe2O4, excellent fluidity and surface plasmon resonance effect of Ag0 nanoparticles, which led to higher separation efficiency of photogenerated electrons and holes, thereby enhancing the hybrids' photocatalytic activity.

  14. Commercial Building Energy Saver: An energy retrofit analysis toolkit

    International Nuclear Information System (INIS)

    Hong, Tianzhen; Piette, Mary Ann; Chen, Yixing; Lee, Sang Hoon; Taylor-Lange, Sarah C.; Zhang, Rongpeng; Sun, Kaiyu; Price, Phillip

    2015-01-01

    Highlights: • Commercial Building Energy Saver is a powerful toolkit for energy retrofit analysis. • CBES provides benchmarking, load shape analysis, and model-based retrofit assessment. • CBES covers 7 building types, 6 vintages, 16 climates, and 100 energy measures. • CBES includes a web app, API, and a database of energy efficiency performance. • CBES API can be extended and integrated with third party energy software tools. - Abstract: Small commercial buildings in the United States consume 47% of the total primary energy of the buildings sector. Retrofitting small and medium commercial buildings poses a huge challenge for owners because they usually lack the expertise and resources to identify and evaluate cost-effective energy retrofit strategies. This paper presents the Commercial Building Energy Saver (CBES), an energy retrofit analysis toolkit, which calculates the energy use of a building, identifies and evaluates retrofit measures in terms of energy savings, energy cost savings and payback. The CBES Toolkit includes a web app (APP) for end users and the CBES Application Programming Interface (API) for integrating CBES with other energy software tools. The toolkit provides a rich set of features including: (1) Energy Benchmarking providing an Energy Star score, (2) Load Shape Analysis to identify potential building operation improvements, (3) Preliminary Retrofit Analysis which uses a custom developed pre-simulated database and, (4) Detailed Retrofit Analysis which utilizes real-time EnergyPlus simulations. CBES includes 100 configurable energy conservation measures (ECMs) that encompass IAQ, technical performance and cost data, for assessing 7 different prototype buildings in 16 climate zones in California and 6 vintages. A case study of a small office building demonstrates the use of the toolkit for retrofit analysis. The development of CBES provides a new contribution to the field by providing a straightforward and uncomplicated decision

  15. Phased Retrofits in Existing Homes in Florida Phase II: Shallow Plus Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Parker, D. [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Martin, E. [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States)

    2016-12-01

    Originally published in February 2016, this revision contains an added section called 'Evaluation of Complete Central System Replacement with Inverter-Driven Heat Pump,' which presents the designs and energy savings evaluations of two different schemes wherein inverter-driven systems replaced a home’s existing central system. Analyses for three additional retrofit measures were bolstered by longer-term data; these are described in the sections on supplemental mini-split heat pumps (MSHPs), smart thermostats, and heat pump clothes dryers. The sections on supplemental MSHPs and smart thermostats were also enhanced with larger sample sizes and projections of weather-normalized annual energy savings. Finally, peak system hour energy demand-reduction predictions are provided for all retrofit measures where meaningful impacts could be drawn.

  16. Phased Retrofits in Existing Homes in Florida Phase II: Shallow Plus Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Martin, E. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-12-01

    Originally published in February 2016, this revision contains an added section called 'Evaluation of Complete Central System Replacement with Inverter-Driven Heat Pump,' which presents the designs and energy savings evaluations of two different schemes wherein inverter-driven systems replaced a home's existing central system. Analyses for three additional retrofit measures were bolstered by longer-term data; these are described in the sections on supplemental mini-split heat pumps (MSHPs), smart thermostats, and heat pump clothes dryers. The sections on supplemental MSHPs and smart thermostats were also enhanced with larger sample sizes and projections of weather-normalized annual energy savings. Finally, peak system hour energy demand-reduction predictions are provided for all retrofit measures where meaningful impacts could be drawn.

  17. Measure Guideline. Deep Energy Enclosure Retrofit for Interior Insulation of Masonry Walls

    Energy Technology Data Exchange (ETDEWEB)

    Musunuru, S. [Building Science Corporation, Westford, MA (United States); Pettit, B. [Building Science Corporation, Westford, MA (United States)

    2015-04-30

    This Measure Guideline describes a deep energy enclosure retrofit solution for insulating mass masonry buildings from the interior. It describes the retrofit assembly, technical details, and installation sequence for retrofitting masonry walls. Interior insulation of masonry retrofits might adversely affect the durability of the wall. This guideline includes a review of decision criteria pertinent to retrofitting masonry walls from the interior and the possible risk of freeze-thaw damage.

  18. Measure Guideline: Deep Energy Enclosure Retrofit for Interior Insulation of Masonry Walls

    Energy Technology Data Exchange (ETDEWEB)

    Musunuru, S. [Building Science Corporation, Westford, MA (United States); Pettit, B. [Building Science Corporation, Westford, MA (United States)

    2015-04-01

    This Measure Guideline describes a deep energy enclosure retrofit (DEER) solution for insulating mass masonry buildings from the interior. It describes the retrofit assembly, technical details, and installation sequence for retrofitting masonry walls. Interior insulation of masonry retrofits has the potential to adversely affect the durability of the wall; this document includes a review of decision criteria pertinent to retrofitting masonry walls from the interior and the possible risk of freeze-thaw damage.

  19. Lux level enhancement and reduction in electricity cost in commercial buildings by retrofitting with PMR luminaries

    International Nuclear Information System (INIS)

    Mariun, N.; Mohibullah; Jasni, J.; Lam, S.Y.

    2006-01-01

    Most of the existing commercial buildings are illuminated by luminaries systems during broad daylight and night which is provided by the renowned lighting industry. However, back in 1980s, the installed luminaries within the office compound were limited in choice of luminaire selection and cost factor impact. Some of the old commercial building are still using prismatic acrylic lens diffuser luminaries in order to brighten up the building for their business activities and a large number of luminaries are needed to illuminate equivalent illumination level as per requirement of the building bye-laws code. With the advancement in luminaries technology, the lighting industries have offered better solution to reduce energy costs by 50% or more, also able to improve the quality of light and reducing the quantity of luminaries requirement by introducing the parabolic mirror reflector (PMR) luminaries system. The selected commercial building as a case study to support this luminaries retrofitting program by comparing the existing luminaries with the retrofit luminaries in terms of the lux measurement and energy cost saving calculation is presented in this paper. Nevertheless, some general lighting design principle rules are also discussed

  20. Light

    CERN Document Server

    Ditchburn, R W

    1963-01-01

    This classic study, available for the first time in paperback, clearly demonstrates how quantum theory is a natural development of wave theory, and how these two theories, once thought to be irreconcilable, together comprise a single valid theory of light. Aimed at students with an intermediate-level knowledge of physics, the book first offers a historical introduction to the subject, then covers topics such as wave theory, interference, diffraction, Huygens' Principle, Fermat's Principle, and the accuracy of optical measurements. Additional topics include the velocity of light, relativistic o

  1. Changes in the Xanthophyll Cycle and Fluorescence Quenching Indicate Light-Dependent Early Events in the Action of Paraquat and the Mechanism of Resistance to Paraquat in Erigeron canadensis (L.) Cronq1

    Science.gov (United States)

    Váradi, Gyula; Darkó, Éva; Lehoczki, Endre

    2000-01-01

    Violaxanthin de-epoxidation, chlorophyll fluorescence quenching, and photosynthetic O2 evolution in the presence of paraquat (Pq) were studied in intact attached leaves of Pq-susceptible, and Pq-resistant (PqR) biotypes of Erigeron canadensis under different light conditions. Initially, similar changes were induced in the two biotypes, but the effects relaxed only in the PqR plants, indicating a Pq elimination process. The penetration of Pq into the chloroplasts of PqR plants proved to be somewhat restricted and highly light-dependent, as revealed by both the light response curves of violaxanthin de-epoxidation and fluorescence quenching and the short-term high-light pre-illumination experiments. An irregular down-regulation of the non-photochemical fluorescence quenching processes was observed, reflected by lower steady-state zeaxanthin and non-photochemical fluorescence quenching levels as compared with the corresponding non-treated high-light controls. It is concluded that light is essential not only for the initiation of the mechanism of resistance to Pq, but also for the penetration of Pq into the chloroplasts in the PqR E. canadensis. Also, the Pq elimination process may cause a modification to the regulation of the non-radiative energy dissipation in PqR plants in the presence of Pq. PMID:10938362

  2. Seeing the light: the effects of particles, dissolved materials, and temperature on in situ measurements of DOM fluorescence in rivers and streams

    Science.gov (United States)

    Downing, Bryan D.; Pellerin, Brian A.; Bergamaschi, Brian A.; Saraceno, John Franco; Kraus, Tamara E.C.

    2012-01-01

    Field-deployable sensors designed to continuously measure the fluorescence of colored dissolved organic matter (FDOM) in situ are of growing interest. However, the ability to make FDOM measurements that are comparable across sites and over time requires a clear understanding of how instrument characteristics and environmental conditions affect the measurements. In particular, the effects of water temperature and light attenuation by both colored dissolved material and suspended particles may be significant in settings such as rivers and streams. Using natural standard reference materials, we characterized the performance of four commercially-available FDOM sensors under controlled laboratory conditions over ranges of temperature, dissolved organic matter (DOM) concentrations, and turbidity that spanned typical environmental ranges. We also examined field data from several major rivers to assess how often attenuation artifacts or temperature effects might be important. We found that raw (uncorrected) FDOM values were strongly affected by the light attenuation that results from dissolved substances and suspended particles as well as by water temperature. Observed effects of light attenuation and temperature agreed well with theory. Our results show that correction of measured FDOM values to account for these effects is necessary and feasible over much of the range of temperature, DOM concentration, and turbidity commonly encountered in surface waters. In most cases, collecting high-quality FDOM measurements that are comparable through time and between sites will require concurrent measurements of temperature and turbidity, and periodic discrete sample collection for laboratory measurement of DOM.

  3. Amelioration and retrofitting of educational buildings

    Science.gov (United States)

    Casciati, Fabio; Casciati, Sara

    2018-01-01

    Following a seismic event that occurred years ago in Central Italy, the public opinion was growing and growing a concern on the adequacy of educational buildings all across Italy. This activated several political decisions and a consequent technical effort is in progress. Technically speaking one has to manage the classical problem of retrofitting existing buildings. However, the legal environment goes across national codes, targeted guidelines and the professional need of achieving pragmatic solutions based on ethical and social acceptation schemes.This paper introduces the topic in its worldwide exception and focuses then on some operative aspects in the Italian situation. It outlines the consolidated steps along this technical process and emphasizes the weak aspects one meets when going across the designers' reports.

  4. Retrofitting reciprocating compressors for noise control

    International Nuclear Information System (INIS)

    Frank, L.; Qualfe, R.

    1992-01-01

    The Alberta Energy Resources Conservation Board recently enacted their noise control directive ID 88-1. The effects of this regulation on the operation of an oil and gas facility are discussed, and a specific case history is presented to provide a disciplined strategy for noise attenuation retrofits. An investigation was carried out into sound sources at a reciprocating compressor gas plant, revealing several sound sources: engine exhaust stacks, engine exhaust silencer shells, direct-drive fan cooler inlets, direct drive fan cooler outlets, aerial cooler inlets and aerial cooler outlets. Details are presented of the investigative techniques and order-ranking of sources by decibel level. When controlling engine exhaust noise, silencers or mufflers are the preferred treatment. Choice of type (reactive or absorptive) and specification of acoustical performance of a silencer are discussed. The gas plant achieved noise reductions of 6-13 dB, measured at affected residences, through the use of engine exhaust silencers. 4 figs., 2 tabs

  5. Retrofitting the Southeast. The Cool Energy House

    Energy Technology Data Exchange (ETDEWEB)

    Zoeller, W. [Steven Winter Associates, Inc., Norwalk, CT (United States); Shapiro, C. [Steven Winter Associates, Inc., Norwalk, CT (United States); Vijayakumar, G. [Steven Winter Associates, Inc., Norwalk, CT (United States); Puttagunta, S. [Steven Winter Associates, Inc., Norwalk, CT (United States)

    2013-02-01

    The Consortium for Advanced Residential Buildings research team has provided the technical engineering and building science support for a highly visible demonstration home that was unveiled at the National Association of Home Builders' International Builders Show on Feb. 9, 2012, in Orlando, FL. The two previous projects, the Las Vegas net-zero ReVISION House and the 2011 VISION and ReVISION Houses in Orlando, met goals for energy efficiency, cost effectiveness, and information dissemination through multiple web-based venues. This report describes the deep energy retrofit of the Cool Energy House (CEH), which began as a mid-1990s two-story traditional specification house of about 4,000 ft2 in the upscale Orlando suburb of Windermere.

  6. Retrofitting the Southeast: The Cool Energy House

    Energy Technology Data Exchange (ETDEWEB)

    Zoeller, W.; Shapiro, C.; Vijayakumar, G.; Puttagunta, S.

    2013-02-01

    The Consortium for Advanced Residential Buildings has provided the technical engineering and building science support for a highly visible demonstration home in connection with the National Association of Home Builders' International Builders Show. The two previous projects, the Las Vegas net-zero ReVISION House and the 2011 VISION and ReVISION Houses in Orlando, met goals for energy efficiency, cost effectiveness, and information dissemination through multiple web-based venues. This project, which was unveiled at the 2012 International Builders Show in Orlando on February 9, is the deep energy retrofit Cool Energy House (CEH). The CEH began as a mid-1990s two-story traditional specification house of about 4,000 ft2 in the upscale Orlando suburb of Windermere.

  7. Design Concept for the Retrofit KAO 1m Robotic Telescope

    Directory of Open Access Journals (Sweden)

    Wonyong Han

    2000-12-01

    Full Text Available Korea Astronomy Observatory (KAO is working to retrofit its 1m robotic telescope in collaboration with a company (ACE, Astronomical Consultants & Equipment. The telescope system is being totally refurbished to make a fully automatic telescope which can operate in both interactive and fully autonomous robotic modes. Progress has been made in design and manufacturing of the telescope mount, mechanics, and optical performance system tests are being made for re-configured primary and secondary mirrors. The optical system is designed to collect 80% incident light within 0.5 arcsec with f/7.5 Ritchey-Chretien design. The telescope mount is an equatorial fork with a friction drive system. The design allows fully programmable tracking speeds with typical range of 15 arcsec/sec with accuracy of +/-5 arcsec/hour. The mount system has integral pointing model software to correct for refraction, and all mechanical errors and misalignments. The pointing model will permit positioning to better than 30 arcsec RMS within 75o from zenith and 45 arcsec RMS elsewhere on the sky. The software is designed for interactive, remote and robotic modes of operation. In interactive and remote mode the user can manually enter coordinates or retrieve them from a computer file. In robotic mode the telescope controller downloads the coordinates in the order determined by the scheduler. The telescope will be equipped with a CCD camera and will be accessible via the internet.

  8. Direct visualization of nucleolar G-quadruplexes in live cells by using a fluorescent light-up probe.

    Science.gov (United States)

    Zhang, Suge; Sun, Hongxia; Chen, Hongbo; Li, Qian; Guan, Aijiao; Wang, Lixia; Shi, Yunhua; Xu, Shujuan; Liu, Meirong; Tang, Yalin

    2018-05-01

    Direct detection of G-quadruplexes in human cells has become an important issue due to the vital role of G-quadruplex related to biological functions. Despite several probes have been developed for detection of the G-quadruplexes in cytoplasm or whole cells, the probe being used to monitor the nucleolar G-quadruplexes is still lacking. Formation of the nucleolar G-quadruplex structures was confirmed by using circular dichroism (CD) spectroscopy. The binding affinity and selectivity of Thioflavin T (ThT) towards various DNA/RNA motifs in solution and gel system were measured by using fluorescence spectroscopy and polyacrylamide gel electrophoresis (PAGE), respectively. G-quadruplex imaging in live cells was directly captured by using confocal laser scanning microscopy (CLSM). Formation of the rDNA and rRNA G-quadruplex structures is demonstrated in vitro. ThT is found to show much higher affinity and selectivity towards these G-quadruplex structures versus other nucleic acid motifs either in solution or in gel system. The nucleolar G-quadruplexes in living cells are visualized by using ThT as a fluorescent probe. G-quadruplex-ligand treatments in live cells lead to sharp decrease of ThT signal. The natural existence of the G-quadruplexes structure in the nucleoli of living cells is directly visualized by using ThT as an indicator. The research provides substantive evidence for formation of the rRNA G-quadruplex structures, and also offers an effective probe for direct visualization of the nucleolar G-quadruplexes in living cells. Copyright © 2018. Published by Elsevier B.V.

  9. Novel lanthanide pH fluorescent probes based on multiple emissions and its visible-light-sensitized feature

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Jintai [School of Chemistry and Environment, South China Normal University, Guangzhou 510006 (China); Zheng, Yuhui, E-mail: yhzheng78@scnu.edu.cn [School of Chemistry and Environment, South China Normal University, Guangzhou 510006 (China); Wang, Qianming, E-mail: qmwang@scnu.edu.cn [Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry and Environment, South China Normal University, Guangzhou 510006 (China); School of Chemistry and Environment, South China Normal University, Guangzhou 510006 (China); Guangzhou Key Laboratory of Materials for Energy Conversion and Storage, Guangzhou 510006 (China); State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082 (China); Zeng, Zhi [School of Chemistry and Environment, South China Normal University, Guangzhou 510006 (China); Zhang, Cheng Cheng [Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, Dallas (United States)

    2014-08-11

    Graphical abstract: A new type of Eu(III) ofloxacin complex as the fluorescent pH indicator has been reported. Compared to pure ligand, the complex offers more distinguished color changes (green–red–blue) derived from both lanthanide line emissions and the secondary ionization steps of ofloxacin. - Highlights: • The pH probe offers a very wide working range in water (pH 1–14). • The emission changes have multiple colors. • Long-lived excited state lifetimes of Eu(III) has been used. • Two types of pH sensitive hydrogels were fabricated. - Abstract: A new type of Eu(III) ofloxacin complex as the fluorescent pH indicator has been presented. Compared to pure ligand, the complex offers more distinguished color changes (green–red–blue) derived from both lanthanide line emissions and the secondary ionization steps of ofloxacin. During the concentration dependence experiments, the photoluminescence studies on the complex showed that the excitation of this pH probe can occur at a very long wavelength which extends to visible range (Ex = 427 nm). Furthermore, the functional complex was successfully incorporated into soft networks and two novel luminescent hydrogels (rod and film) were fabricated. The soft materials also exhibited specific responses towards the pH variation. Finally, the onion cell-stain experiments were carried out to further confirm the validity of pH dependence and the results support the idea that the material will be suitable for monitoring biological samples in the future.

  10. Effect of Percent Relative Humidity, Moisture Content, and Compression Force on Light-Induced Fluorescence (LIF) Response as a Process Analytical Tool.

    Science.gov (United States)

    Shah, Ishan G; Stagner, William C

    2016-08-01

    The effect of percent relative humidity (16-84% RH), moisture content (4.2-6.5% w/w MC), and compression force (4.9-44.1 kN CF) on the light-induced fluorescence (LIF) response of 10% w/w active pharmaceutical ingredient (API) compacts is reported. The fluorescent response was evaluated using two separate central composite designs of experiments. The effect of % RH and CF on the LIF signal was highly significant with an adjusted R (2)  = 0.9436 and p relative humidity (p = 0.0022), CF (p < 0.0001), and % RH(2) (p = 0.0237) were statistically significant factors affecting the LIF response. The effects of MC and CF on LIF response were also statistically significant with a p value <0.0001 and adjusted R (2) value of 0.9874. The LIF response was highly impacted by MC (p < 0.0001), CF (p < 0.0001), and MC(2) (p = 0022). At 10% w/w API, increased % RH, MC, and CF led to a nonlinear decrease in LIF response. The derived quadratic model equations explained more than 94% of the data. Awareness of these effects on LIF response is critical when implementing LIF as a process analytical tool.

  11. ReViMS: Software tool for estimating the volumes of 3-D multicellular spheroids imaged using a light sheet fluorescence microscope.

    Science.gov (United States)

    Piccinini, Filippo; Tesei, Anna; Zanoni, Michele; Bevilacqua, Alessandro

    2017-11-01

    Cancer 3-D spheroids are widely used to test drugs and radiotherapy treatments. These 3-D cell clusters range from tens to hundreds of micrometers in size, with shapes that typically differ from a perfect sphere. Change in spheroid volume is one of the most important parameters for evaluating treatment efficacy, and using light sheet fluorescence microscopes (LSFM), optical sections of samples in that size range can be obtained. However, there remains a lack of validated methods for quantifying the volumes of 3-D multicellular aggregates. Here, we present Reconstruction and Visualization from Multiple Sections (ReViMS), an open-source, user-friendly software for automatically segmenting z-stacks of fluorescence images and estimating the volumes of 3-D multicellular spheroids. To assess the precision and accuracy of the volume estimates obtained with ReViMS, we used several cancer spheroids imaged with LSFM. Both the precision and accuracy were >95%, demonstrating the effectiveness of ReViMS.

  12. Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope.

    Science.gov (United States)

    Guan, Zeyi; Lee, Juhyun; Jiang, Hao; Dong, Siyan; Jen, Nelson; Hsiai, Tzung; Ho, Chih-Ming; Fei, Peng

    2016-01-01

    We developed a compact plane illumination plugin (PIP) device which enabled plane illumination and light sheet fluorescence imaging on a conventional inverted microscope. The PIP device allowed the integration of microscope with tunable laser sheet profile, fast image acquisition, and 3-D scanning. The device is both compact, measuring approximately 15 by 5 by 5 cm, and cost-effective, since we employed consumer electronics and an inexpensive device molding method. We demonstrated that PIP provided significant contrast and resolution enhancement to conventional microscopy through imaging different multi-cellular fluorescent structures, including 3-D branched cells in vitro and live zebrafish embryos. Imaging with the integration of PIP greatly reduced out-of-focus contamination and generated sharper contrast in acquired 2-D plane images when compared with the stand-alone inverted microscope. As a result, the dynamic fluid domain of the beating zebrafish heart was clearly segmented and the functional monitoring of the heart was achieved. Furthermore, the enhanced axial resolution established by thin plane illumination of PIP enabled the 3-D reconstruction of the branched cellular structures, which leads to the improvement on the functionality of the wide field microscopy.

  13. Highly Efficient White Organic Light-Emitting Diodes with Controllable Excitons Behavior by a Mixed Interlayer between Fluorescence Blue and Phosphorescence Yellow-Emitting Layers

    Directory of Open Access Journals (Sweden)

    Chun-Hong Gao

    2013-01-01

    Full Text Available A highly efficient hybrid white organic light-emitting diode (HWOLED has been demonstrated with a mixed interlayer between fluorescent blue and phosphorescent yellow-emitting layers. The device structure is simplified by using a controllable fluorescence-mixed interlayer-phosphorescence emission layer structure. The electroluminance (EL performance can be modulated easily by adjusting the ratio of the hole-predominated material to the electron-predominated material in the interlayer. It is found that the HWOLED with a ratio of 3 : 2 exhibits a current efficiency of 34 cd/A and a power efficiency of 29 lm/W at 1000 cd/m2 with warm white Commission Internationale de l’Eclairage (CIE1931 coordinates of (0.4273, 0.4439. The improved efficiency and adaptive CIE coordinates are attributed to the controllable mixed interlayer with enhanced charge carrier transport, optimized excitons distribution, and improved harvestings of singlet and triplet excitons.

  14. Benzimidazobenzothiazole-Based Bipolar Hosts to Harvest Nearly All of the Excitons from Blue Delayed Fluorescence and Phosphorescent Organic Light-Emitting Diodes.

    Science.gov (United States)

    Cui, Lin-Song; Kim, Jong Uk; Nomura, Hiroko; Nakanotani, Hajime; Adachi, Chihaya

    2016-06-06

    Much effort has been devoted to developing highly efficient organic light-emitting diodes (OLEDs) that function through phosphorescence or thermally activated delayed fluorescence (TADF). However, efficient host materials for blue TADF and phosphorescent guest emitters are limited because of their requirement of high triplet energy levels. Herein, we report the rigid acceptor unit benzimidazobenzothiazole (BID-BT), which is suitable for use in bipolar hosts in blue OLEDs. The designed host materials, based on BID-BT, possess high triplet energy and bipolar carrier transport ability. Both blue TADF and phosphorescent OLEDs containing BID-BT-based derivatives exhibit external quantum efficiencies as high as 20 %, indicating that these hosts allow efficient triplet exciton confinement appropriate for blue TADF and phosphorescent guest emitters. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy [version 1; referees: 2 approved, 1 approved with reservations

    Directory of Open Access Journals (Sweden)

    Elisabeth Brama

    2016-12-01

    Full Text Available In-resin fluorescence (IRF protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution correlation of fluorophore to organelle. IRF samples also offer a unique opportunity to automate correlative imaging workflows. Here we present two new locator tools for finding and following fluorescent cells in IRF blocks, enabling future automation of correlative imaging. The ultraLM is a fluorescence microscope that integrates with an ultramicrotome, which enables ‘smart collection’ of ultrathin sections containing fluorescent cells or tissues for subsequent transmission electron microscopy or array tomography. The miniLM is a fluorescence microscope that integrates with serial block face scanning electron microscopes, which enables ‘smart tracking’ of fluorescent structures during automated serial electron image acquisition from large cell and tissue volumes.

  16. Drivers for retrofit: a sociocultural approach to houses and inhabitants

    DEFF Research Database (Denmark)

    Tjørring, Lise; Gausset, Quentin

    2018-01-01

    , retrofits were investigated from the perspective of the everyday life of homeowners. Three social dimensions that played an important role when the people in the study discussed house renovation were identified and discussed: houses as part of homeowners’ life cycles and personal events; houses......Private households constitute approximately 30% of the total energy consumption in Denmark. The greatest potential for reducing energy consumption in private households is through retrofits of existing houses. One of the most common ways to promote retrofits is the act of sending an energy advisor...... as frameworks for social relations; and houses as markers of social values and status. These results indicate a need to develop new strategies to promote retrofits more effectively, which focus less on the techno-economic aspects and more on the social dimensions....

  17. DOE Webinar - Residential Geothermal Heat Pump Retrofits (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, E. R.

    2010-12-14

    This presentation was given December 14, 2010, as part of DOE's Webinar series. The presentation discusses geothermal heat pump retrofits, technology options, and an overview of geothermal energy and geothermal heat pumps.

  18. Transit safety retrofit package development : applications requirements document.

    Science.gov (United States)

    2014-05-01

    This Application Requirements Document for the Transit Safety Retrofit Package (TRP) Development captures the : system, hardware and software requirements towards fulfilling the technical objectives stated within the contract. : To achieve the object...

  19. Building Performance Simulation tools for planning of energy efficiency retrofits

    DEFF Research Database (Denmark)

    Mondrup, Thomas Fænø; Karlshøj, Jan; Vestergaard, Flemming

    2014-01-01

    Designing energy efficiency retrofits for existing buildings will bring environmental, economic, social, and health benefits. However, selecting specific retrofit strategies is complex and requires careful planning. In this study, we describe a methodology for adopting Building Performance...... to energy efficiency retrofits in social housing. To generate energy savings, we focus on optimizing the building envelope. We evaluate alternative building envelope actions using procedural solar radiation and daylight simulations. In addition, we identify the digital information flow and the information...... Simulation (BPS) tools as energy and environmentally conscious decision-making aids. The methodology has been developed to screen buildings for potential improvements and to support the development of retrofit strategies. We present a case study of a Danish renovation project, implementing BPS approaches...

  20. Short-Term Test Results. Multifamily Home Energy Efficiency Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Lyons, James [Building America Partnership for Improved Residential Construction (BA-PIRC), Cocoa, FL (United States)

    2013-01-01

    Multifamily deep energy retrofits (DERs) represent great potential for energy savings, while also providing valuable insights on research-generated efficiency measures, cost-effectiveness metrics, and risk factor strategies for the multifamily housing industry. This report describes the Bay Ridge project, a base scope retrofit with a goal of achieving 30% savings (relative to pre-retrofit), and a DER scope with a goal of 50% savings (relative to pre-retrofit). Findings from the short-term testing at Bay Ridge include air infiltration reductions of greater than 60% in the DER building; a hybrid heat pump system with a Savings to Investment Ratio (SIR) > 1 (relative to a high efficiency furnace) which also provides the resident with added incentive for energy savings; and duct leakage reductions of > 60% using an aerosolized duct sealing approach.

  1. Transit safety retrofit package development : TRP concept of operations.

    Science.gov (United States)

    2014-05-01

    This document describes the Concept of Operations (ConOps) for the Transit Safety Retrofit Package (TRP). The : ConOps describes the current state of operations with respect to the integration of Connected Vehicle technology in : transit buses, estab...

  2. Method for Determining Optimal Residential Energy Efficiency Retrofit Packages

    Energy Technology Data Exchange (ETDEWEB)

    Polly, B.; Gestwick, M.; Bianchi, M.; Anderson, R.; Horowitz, S.; Christensen, C.; Judkoff, R.

    2011-04-01

    Businesses, government agencies, consumers, policy makers, and utilities currently have limited access to occupant-, building-, and location-specific recommendations for optimal energy retrofit packages, as defined by estimated costs and energy savings. This report describes an analysis method for determining optimal residential energy efficiency retrofit packages and, as an illustrative example, applies the analysis method to a 1960s-era home in eight U.S. cities covering a range of International Energy Conservation Code (IECC) climate regions. The method uses an optimization scheme that considers average energy use (determined from building energy simulations) and equivalent annual cost to recommend optimal retrofit packages specific to the building, occupants, and location. Energy savings and incremental costs are calculated relative to a minimum upgrade reference scenario, which accounts for efficiency upgrades that would occur in the absence of a retrofit because of equipment wear-out and replacement with current minimum standards.

  3. Masonry Bearing and Shear Walls Retrofitted With Overlay Composite Material

    National Research Council Canada - National Science Library

    Al-Chaar, Ghassan

    1998-01-01

    ...) switch-house structures have been found to be vulnerable to earthquakes. One technique to reduce the seismic vulnerability of these structures is to retrofit the masonry walls using the Hexcel-Fyfe TYFO(TM) system...

  4. Attic Retrofits Using Nail-Base Insulated Panels

    Energy Technology Data Exchange (ETDEWEB)

    Mallay, David [Home Innovation Research Labs; Kochkin, Vladimir [Home Innovation Research Labs

    2018-03-26

    This project developed and demonstrated a roof/attic energy retrofit solution using nail-base insulated panels for existing homes where traditional attic insulation approaches are not effective or feasible. Nail-base insulated panels (retrofit panels) consist of rigid foam insulation laminated to one face of a wood structural panel. The prefabricated panels are installed above the existing roof deck during a reroofing effort.

  5. Building America Case Study: Retrofitting a 1960s Split-Level, Cold-Climate Home, Westport, Connecticut; Whole-House Solutions for Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-08-01

    ??National programs such as Home Performance with ENERGY STAR(R) and numerous other utility air sealing programs have brought awareness to homeowners of the benefits of energy efficiency retrofits. Yet, these programs tend to focus on the low-hanging fruit: air-sealing the thermal envelope and ductwork where accessible, switch to efficient lighting, and low-flow fixtures. At the other end of the spectrum, deep-energy retrofit programs are also being encouraged by various utilities across the country. While deep energy retrofits typically seek 50% energy savings, they are often quite costly and most applicable to gut-rehab projects. A significant potential for lowering energy usage in existing homes lies between the low hanging fruit and deep energy retrofit approaches - retrofits that save approximately 30% in energy over the existing conditions. A key is to be non-intrusive with the efficiency measures so the retrofit projects can be accomplished in occupied homes. This cold climate retrofit project involved the design and optimization of a home in Connecticut that sought to improve energy savings by at least 30% (excluding solar PV) over the existing home's performance. This report documents the successful implementation of a cost-effective solution package that achieved performance greater than 30% over the pre-retrofit - what worked, what did not, and what improvements could be made. Confirmation of successfully achieving 30% source energy savings over the pre-existing conditions was confirmed through energy modeling and comparison of the utility bills pre- and post- retrofit.

  6. Retrofit of a Multifamily Mass Masonry Building in New England

    Energy Technology Data Exchange (ETDEWEB)

    Ueno, K.; Kerrigan, P.; Wytrykowska, H.; Van Straaten, R.

    2013-08-01

    Merrimack Valley Habitat for Humanity (MVHfH) has partnered with Building Science Corporation to provide high performance affordable housing for 10 families in the retrofit of an existing brick building (a former convent) into condominiums. The research performed for this project provides information regarding advanced retrofit packages for multi-family masonry buildings in Cold climates. In particular, this project demonstrates safe, durable, and cost-effective solutions that will potentially benefit millions of multi-family brick buildings throughout the East Coast and Midwest (Cold climates). The retrofit packages provide insight on the opportunities for and constraints on retrofitting multifamily buildings with ambitious energy performance goals but a limited budget. The condominium conversion project will contribute to several areas of research on enclosures, space conditioning, and water heating. Enclosure items include insulation of mass masonry building on the interior, airtightness of these types of retrofits, multi-unit building compartmentalization, window selection, and roof insulation strategies. Mechanical system items include combined hydronic and space heating systems with hydronic distribution in small (low load) units, and ventilation system retrofits for multifamily buildings.

  7. Innovative Retrofit Insulation Strategies for Concrete Masonry Foundations

    Energy Technology Data Exchange (ETDEWEB)

    Huelman, P. [Univ. of Minnesota, St. Paul, MN (United States). NorthernSTAR Building America Partnership; Goldberg, L. [Univ. of Minnesota, St. Paul, MN (United States). NorthernSTAR Building America Partnership; Jacobson, R. [Univ. of Minnesota, St. Paul, MN (United States). NorthernSTAR Building America Partnership

    2015-05-06

    This study was designed to test a new approach for foundation insulation retrofits, with the goal of demonstrating improved moisture control, improved occupant comfort, and reduced heat loss. Because conducting experimental research on existing below-grade assemblies is very difficult, most of the results are based on simulations. The retrofit approach consists of filling open concrete block cores with an insulating material and adding R-10 exterior insulation that extends 1 ft below grade. The core fill is designed to improve the R-value of the foundation wall and increase the interior wall surface temperature, but more importantly to block convection currents that could otherwise increase moisture loads on the foundation wall and interior space. The exterior insulation significantly reduces heat loss through the most exposed part of the foundation and further increases the interior wall surface temperature. This improves occupant comfort and decreases the risk of condensation. Such an insulation package avoids the full-depth excavation necessary for exterior insulation retrofits, reduces costs, and eliminates the moisture and indoor air quality risks associated with interior insulation retrofits. Retrofit costs for the proposed approach were estimated at roughly half those of a full-depth exterior insulation retrofit.

  8. Wyandotte Neighborhood Stabilization Program: Retrofit of Two Homes

    Energy Technology Data Exchange (ETDEWEB)

    Lukachko, A.; Grin, A.; Bergey, D.

    2013-04-01

    The Wyandotte NSP2 project aims to build 20 new houses and retrofit 20 existing houses in Wyandotte, MI. This report will detail the retrofit of 2 existing houses in the program. Wyandotte is part of a Michigan State Housing Development Authority-led consortium that is funded by HUD under the NSP2 program. The City of Wyandotte has also been awarded DOE EE&CBG funds that are being used to develop a district GSHP system to service the project. This draft report examines the energy efficiency recommendations for retrofit construction at these homes. The report will be of interest to anyone planning an affordable, high performance retrofit of an existing home in a Cold Climate zone. Information from this report will also be useful to retrofit or weatherization program staff as some of the proposed retrofit solutions will apply to a wide range of projects. Preliminary results from the first complete house suggest that the technology package employed (which includes spray foam insulation and insulating sheathing) does meet the specific whole house water, air, and thermal control requirements, as well as, the project's affordability goals. Monitoring of the GSHP system has been recommended and analysis of this information is not yet available.

  9. High-throughput growth prediction for Lactuca sativa L. seedlings using chlorophyll fluorescence in a plant factory with artificial lighting

    Directory of Open Access Journals (Sweden)

    Shogo eMoriyuki

    2016-03-01

    Full Text Available Poorly grown plants that result from differences in individuals lead to large profit losses for plant factories that use large electric power sources for cultivation. Thus, identifying and culling the low-grade seedlings at an early stage, using so-called seedling diagnosis technology, plays an important role in avoiding large losses in plant factories. In this study, we developed a high-throughput diagnosis system using the measurement of chlorophyll fluorescence (CF in a commercial large-scale plant factory, which produces about 5,000 lettuce plants every day. At an early stage (6 days after sowing, a CF image of 6,000 seedlings was captured every 4 hours on the final greening day by a high-sensitivity CCD camera and an automatic transferring machine, and biological indices were extracted. Using machine learning, plant growth can be predicted with a high degree of accuracy based on biological indices including leaf size, amount of CF, and circadian rhythms in CF. Growth prediction was improved by addition of temporal information on CF. The present data also provide new insights into the relationships between growth and temporal information regulated by the inherent biological clock.

  10. EFFECT OF Cu AND Mn TOXICITY ON CHLOROPHYLL FLUORESCENCE AND GAS EXCHANGE IN RICE AND SUNFLOWER UNDER DIFFERENT LIGHT INTENSITIES

    Directory of Open Access Journals (Sweden)

    Hajiboland R.

    2007-06-01

    Full Text Available Copper (Cu and manganese (Mn are essential micronutrients for plants, but toxic at high concentrations. Responses of rice (Oryza sativa L. and sunflower (Helianthus annuus L. to toxic concentrations of Mn and Cu (up to 100 μM were studied under three light intensities including low (LL, PPFD=100, intermediate (IL, PPFD=500 and high (HL, PPFD=800 light intensities in hydroponic medium. Rice plants showed higher susceptibility than sunflower to both heavy metals concerning dry matter of shoot and root. Growing under higher light intensity strengthened the effect of Cu toxicity while ameliorated that of Mn, the latter was attributed to the lower Mn accumulation of HL plants in both shoot and root. Chlorophyll content of leaves was influenced negatively only by Cu treatment and that at the highest concentration in the medium (100 μM. Similar with growth results, reduction of net assimilation rate (A was higher in HL than LL plants treated by excess Cu, but in contrast to growth response, reduction was more prominent in sunflower than rice. Excess Mn-induced reduction of A was similar between LL and HL plants and was greater in sunflower than rice. Reduction of A was partly attributable to stomatal limitation, but non-stomatal mechanisms were also involved in this reduction. Copper and Mn treatment did not change the optimal quantum efficiency of PSII in dark-adapted chloroplasts (Fv/Fm ratio, but Fv/F0 was influenced particularly by Cu treatment, the reduction was higher in rice than sunflower and in HL compared to LL plants. Regarding excess Cu and Mn-mediated alterations in chlorophyll concentration, Fv/F0 and Tm values, it was suggested that, Cu and Mn toxicity depress the leaf photosynthetic capacity primarily by causing a significant alteration of the composition and functional competence of the photosynthetic units rather a reduction in the number of photosynthetic units (PSUs per unit leaf area.

  11. Bactericidal Activity of Aqueous Acrylic Paint Dispersion for Wooden Substrates Based on TiO2 Nanoparticles Activated by Fluorescent Light

    Directory of Open Access Journals (Sweden)

    Diana Di Gioia

    2013-08-01

    Full Text Available The photocatalytic effect of TiO2 has great potential for the disinfection of surfaces. Most studies reported in the literature use UV activation of TiO2, while visible light has been used only in a few applications. In these studies, high concentrations of TiO2, which can compromise surface properties, have been used. In this work, we have developed an acrylic-water paint dispersion containing low TiO2 content (2 vol % for the inactivation of microorganisms involved in hospital-acquired infections. The nanoparticles and the coating have been characterized using spectroscopic techniques and transmission electron microscopy, showing their homogenous dispersion in the acrylic urethane coating. A common fluorescent light source was used to activate the photocatalytic activity of TiO2. The paint dispersion showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The coating containing the TiO2 nanoparticles maintained good UV stability, strong adhesion to the substrate and high hardness. Therefore, the approach used is feasible for paint formulation aimed at disinfection of healthcare surfaces.

  12. PORST: a computer code to analyze the performance of retrofitted steam turbines

    Energy Technology Data Exchange (ETDEWEB)

    Lee, C.; Hwang, I.T.

    1980-09-01

    The computer code PORST was developed to analyze the performance of a retrofitted steam turbine that is converted from a single generating to a cogenerating unit for purposes of district heating. Two retrofit schemes are considered: one converts a condensing turbine to a backpressure unit; the other allows the crossover extraction of steam between turbine cylinders. The code can analyze the performance of a turbine operating at: (1) valve-wide-open condition before retrofit, (2) partial load before retrofit, (3) valve-wide-open after retrofit, and (4) partial load after retrofit.

  13. Pilot Residential Deep Energy Retrofits and the PNNL Lab Homes

    Energy Technology Data Exchange (ETDEWEB)

    Widder, Sarah H.; Chandra, Subrato; Parker, Graham B.; Sande, Susan; Blanchard, Jeremy; Stroer, Dennis; McIlvaine, Janet; Chasar, David; Beal, David; Sutherland, Karen

    2012-01-01

    This report summarizes research investigating the technical and economic feasibility of several pilot deep energy retrofits, or retrofits that save 30% to 50% or more on a whole-house basis while increasing comfort, durability, combustion safety, and indoor air quality. The work is being conducted for the U.S. Department of Energy Building Technologies Program as part of the Building America Program. As part of the overall program, Pacific Northwest National Laboratory (PNNL) researchers are collecting and analyzing a comprehensive dataset that describes pre- and post-retrofit energy consumption, retrofit measure cost, health and comfort impacts, and other pertinent information for each home participating in the study. The research and data collection protocol includes recruitment of candidate residences, a thorough test-in audit, home energy modeling, and generation of retrofit measure recommendations, implementation of the measures, test-out, and continued evaluation. On some homes, more detailed data will be collected to disaggregate energy-consumption information. This multi-year effort began in October 2010. To date, the PNNL team has performed test-in audits on 51 homes in the marine, cold, and hot-humid climate zones, and completed 3 retrofits in Texas, 10 in Florida, and 2 in the Pacific Northwest. Two of the retrofits are anticipated to save 50% or more in energy bills and the others - savings are in the 30% to 40% range. Fourteen other retrofits are under way in the three climate zones. Metering equipment has been installed in seven of these retrofits - three in Texas, three in Florida, and one in the Pacific Northwest. This report is an interim update, providing information on the research protocol and status of the PNNL deep energy retrofit project as of December, 2011. The report also presents key findings and lessons learned, based on the body of work to date. In addition, the report summarizes the status of the PNNL Lab Homes that are new

  14. Economic Valuation of Hypothetical Paratransit Retrofitting

    Directory of Open Access Journals (Sweden)

    Naili Huda

    2015-06-01

    Full Text Available This paper describes a feasibility analysis of conventional and retrofitted paratransits, comparing economic performance of conventional paratransit with those using lead acid and lithium batteries. Research object is Dago-Kalapa paratransit in Bandung, West Java, travelling the distance of 11 km in town, under 8 peak hour operation. After calculating the estimated annual cost and benefit; net present value (NPV, payback period (PBP and internal rate of return (IRR then were quantified to provide feasibility description of those three paratransits. In addition, a sensitivity analysis regarding discount rate, gasoline price and battery price is given to offer broader sense of factors embraced. It is found that both gasoline and lead acid paratransit have big NPVs with only slight differences, while lithium paratransit has negative NPV. This phenomenon applies to their PBPs and IRRs as well. Only when gasoline costs reaches IDR 15,000 will electric paratransit prevails over conventional one. Thus, it can be inferred that at the moment, paratransit runs with gasoline is still the most cost effective compared to its counterparts. However, starting retrofitting from now is endorsed due to its environmental benefit. Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin-top:0in; mso-para-margin-right:0in; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}

  15. Rate Constants of PSII Photoinhibition and its Repair, and PSII Fluorescence Parameters in Field Plants in Relation to their Growth Light Environments.

    Science.gov (United States)

    Miyata, Kazunori; Ikeda, Hiroshi; Nakaji, Masayoshi; Kanel, Dhana Raj; Terashima, Ichiro

    2015-09-01

    The extent of photoinhibition of PSII is determined by a balance between the rate of photodamage to PSII and that of repair of the damaged PSII. It has already been indicated that the rate constants of photodamage (kpi) and repair (krec) of the leaves differ depending on their growth light environment. However, there are no studies using plants in the field. We examined these rate constants and fluorescence parameters of several field-grown plants to determine inter-relationships between these values and the growth environment. The kpi values were strongly related to the excess energy, EY, of the puddle model and non-regulated energy dissipation, Y(NO), of the lake model, both multiplied by the photosynthetically active photon flux density (PPFD) level during the photoinhibitory treatment. In contrast, the krec values corrected against in situ air temperature were very strongly related to the daily PPFD level. The plants from the fields showed higher NPQ than the chamber-grown plants, probably because these field plants acclimated to stronger lightflecks than the averaged growth PPFD. Comparing chamber-grown plants and the field plants, we showed that kpi is determined by the incident light level and the photosynthetic capacities such as in situ rate of PSII electron transport and non-photochemical quenching (NPQ) [e.g. Y(NO)×PPFD] and that krec is mostly determined by the growth light and temperature levels. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  16. Green phosphorescent organic light-emitting devices based on different electron transport layers combining with fluorescent sub-monolayer

    Science.gov (United States)

    Yang, Hui-shan; Guo, Hui-yu; Wu, Li-shuang

    2017-03-01

    We report a small molecule host of 4,4(-N,N)-dicarbazole-biphenyl (CBP) doped with 8% tris(2-phenylpyridine) iridium (Irppy3) for use in efficient green phosphorescent organic light-emitting devices (PHOLEDs) combined with different electron transport layers of Alq and BAlq. The PHOLEDs exhibit maximum current efficiency and power efficiency of 19.8 cd/A and 6.21 lm/W, respectively. The high performance of such PHOLEDs is attributed to the better electron mobile ability of BAlq and sub-monolayer quinacridone (QAD) as carrier trapping layer and equal charge carrier mobilities of hole and electron to form the broad carrier recombination zone in the emitting layer, which can reduce the triplet-triplet annihilation and improve the efficiency of the device.

  17. Greenbuilt Retrofit Test House Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Sparn, B. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Hudon, K. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Earle, L. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Booten, C. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Tabares-Velasco, P. C. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Barker, G. [Mountain Energy Partnership, Longmont, CO (United States); Hancock, C. E. [Mountain Energy Partnership, Longmont, CO (United States)

    2014-06-01

    The Greenbuilt house is a 1980's era house in the Sacramento area that was a prominent part of Sacramento Municipal Utility District's (SMUD) Energy Efficient Remodel Demonstration Program. The house underwent an extensive remodel, aimed at improving overall energy efficiency with a goal of reducing the home's energy use by 50%. NREL researchers performed a number of tests on the major systems touched by the retrofit to ensure they were working as planned. Additionally, SMUD rented the house from Greenbuilt Construction for a year to allow NREL to perform a number of tests on the cooling system and the water heating system. The goal of the space conditioning tests was to find the best ways to cut cooling loads and shift the summer peak. The water heating system, comprised of an add-on heat pump water heater and an integrated collector-storage solar water heater, was operated with a number of different draw profiles to see how varying hot water draw volume and schedule affected the performance of the system as a whole. All the experiments were performed with the house empty, with a simulated occupancy schedule running in the house to mimic the load imposed by real occupants.

  18. CRNL research reactor retrofit Emergency Filtration System

    International Nuclear Information System (INIS)

    Philippi, H.M.

    1990-01-01

    This paper presents a brief history of NRX and NRU research reactor effluent air treatment systems before describing the selection and design of an appropriate retrofit Emergency Filtration System (EFS) to serve these reactors and the future MX-10 isotope production reactor. The conceptual design of the EFS began in 1984. A standby concrete shielding filter-adsorber system, sized to serve the reactor with the largest exhaust flow, was selected. The standby system, bypassed under normal operating conditions, is equipped with normal exhaust stream shutoff and diversion valves to be activated manually when an emergency is anticipated, or automatically when emergency levels of gamma radiation are detected in the exhaust stream. The first phase of the EFS installation, that is the construction of the EFS and the connection of NRU to the system, was completed in 1987. The second phase of construction, which includes the connection of NRX and provisions for the future connection of MX-10, is to be completed in 1990

  19. Analysis of signal processing in vestibular circuits with a novel light-emitting diodes-based fluorescence microscope.

    Science.gov (United States)

    Direnberger, Stephan; Banchi, Roberto; Brosel, Sonja; Seebacher, Christian; Laimgruber, Stefan; Uhl, Rainer; Felmy, Felix; Straka, Hans; Kunz, Lars

    2015-05-01

    Optical visualization of neural network activity is limited by imaging system-dependent technical tradeoffs. To overcome these constraints, we have developed a powerful low-cost and flexible imaging system with high spectral variability and unique spatio-temporal precision for simultaneous optical recording and manipulation of neural activity of large cell groups. The system comprises eight high-power light-emitting diodes, a camera with a large metal-oxide-semiconductor sensor and a high numerical aperture water-dipping objective. It allows fast and precise control of excitation and simultaneous low noise imaging at high resolution. Adjustable apertures generated two independent areas of variable size and position for simultaneous optical activation and image capture. The experimental applicability of this system was explored in semi-isolated preparations of larval axolotl (Ambystoma mexicanum) with intact inner ear organs and central nervous circuits. Cyclic galvanic stimulation of semicircular canals together with glutamate- and γ-aminobutyric acid (GABA)-uncaging caused a corresponding modulation of Ca(2+) transients in central vestibular neurons. These experiments revealed specific cellular properties as well as synaptic interactions between excitatory and inhibitory inputs, responsible for spatio-temporal-specific sensory signal processing. Location-specific GABA-uncaging revealed a potent inhibitory shunt of vestibular nerve afferent input in the predominating population of tonic vestibular neurons, indicating a considerable impact of local and commissural inhibitory circuits on the processing of head/body motion-related signals. The discovery of these previously unknown properties of vestibular computations demonstrates the merits of our novel microscope system for experimental applications in the field of neurobiology. © 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.

  20. Assessment and evaluation of timber piles used in Nebraska for retrofit and rating.

    Science.gov (United States)

    2014-04-01

    The cost of an effective retrofitting and rehabilitation of timber piles is less than cost of replacing the piles. However, some of the retrofit options used in Nebraska failed to result in the expected performance levels. Further, design and detaili...

  1. 77 FR 24505 - Hazard Mitigation Assistance for Wind Retrofit Projects for Existing Residential Buildings

    Science.gov (United States)

    2012-04-24

    ...] Hazard Mitigation Assistance for Wind Retrofit Projects for Existing Residential Buildings AGENCY... for Wind Retrofit Projects for Existing Residential Buildings. DATES: Comments must be received by... to protect existing one- and two-family residential buildings (not including manufactured housing...

  2. Retrofitting of Reinforced Concrete Beams using Reactive Powder Concrete (RPC)

    Science.gov (United States)

    Karthik, S.; Sundaravadivelu, Karthik

    2017-07-01

    Strengthening of existing damaged structures is one of the leading studies in civil engineering. The purpose of retrofitting is to structurally treat the member with an aim to restore the structure to its original strength. The focus of this project is to study the behaviour of damaged Reinforced Concrete beam retrofitted with Reactive Powder Concrete (RPC) Overlay. Reinforced concrete beams of length 1200 mm, width 100 mm and depth 200 mm were casted with M30 grade of concrete in the laboratory and cured for 28 days. One beam is taken as control and are tested under two point loading to find out ultimate load. Remaining beams are subjected to 90 % ultimate load of control beams. The partially damaged beams are retrofitted with Reactive Powder Concrete Overlay at the full tension face of the beam and side overlay depends upon the respectable retrofitting techniques with 10 mm and 20 mm thick layer to find optimum. Materials like steel fibres are added to enhance the ductility by eliminating coarse particle for homogeneity of the structure. Finally, the modes of failure for retrofitted beams are analysed experimentally under two point loading & compared the results with Control beam.

  3. Short-Term Test Results: Multifamily Home Energy Efficiency Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Lyons, J.

    2013-01-01

    Multifamily deep energy retrofits (DERs) represent great potential for energy savings, while also providing valuable insights on research-generated efficiency measures, cost-effectiveness metrics, and risk factor strategies for the multifamily housing industry. The Bay Ridge project is comprised of a base scope retrofit with a goal of achieving 30% savings (relative to pre-retrofit), and a DER scope with a goal of 50% savings (relative to pre-retrofit). The base scope has been applied to the entire complex, except for one 12-unit building which underwent the DER scope. Findings from the implementation, commissioning, and short-term testing at Bay Ridge include air infiltration reductions of greater than 60% in the DER building; a hybrid heat pump system with a Savings to Investment Ratio (SIR) > 1 (relative to a high efficiency furnace) which also provides the resident with added incentive for energy savings; and duct leakage reductions of > 60% using an aerosolized duct sealing approach. Despite being a moderate rehab instead of a gut rehab, the Bay Ridge DER is currently projected to achieve energy savings ≥ 50% compared to pre-retrofit, and the short-term testing supports this estimate.

  4. Wyandotte Neighborhood Stabilization Program: Retrofit of Two Homes

    Energy Technology Data Exchange (ETDEWEB)

    Lukachko, A. [Building Science Corporation (BSC), Somerville, MA (United States); Grin, A. [Building Science Corporation (BSC), Somerville, MA (United States); Bergey, D. [Building Science Corporation (BSC), Somerville, MA (United States)

    2013-04-01

    The Wyandotte NSP2 project aims to build 20 new houses and retrofit 20 existing houses in Wyandotte, MI. Wyandotte is part of a Michigan State Housing Development Authority-led consortium that is funded by HUD under the NSP2 program. The City of Wyandotte has also been awarded DOE EE&CBG funds that are being used to develop a district ground source heat pump (GSHP) system to service the project. This report details the retrofit of two existing houses in the program, and examines the energy efficiency recommendations for the homes. The report will be of interest to anyone planning an affordable, high performance retrofit of an existing home in a cold climate zone. Information from this report will also be useful to retrofit or weatherization program staff as some of the proposed retrofit solutions will apply to a wide range of projects. Preliminary results from the first complete house suggest that the technology package employed (which includes spray foam insulation and insulating sheathing) does meet the specific whole house water, air, and thermal control requirements, as well as, the project’s affordability goals. Monitoring of the GSHP system has been recommended and analysis of this information is not yet available.

  5. A study on building performance analysis for energy retrofit of existing industrial facilities

    International Nuclear Information System (INIS)

    Gourlis, Georgios; Kovacic, Iva

    2016-01-01

    Highlights: • Thermal simulation of a historical industrial hall with limited data availability. • Considering waste heat from machinery after measuring production fluctuations. • Test of retrofit alternatives for roof and skylights. • Results indicate a significant reduction in heating energy demand up to 52%. • After retrofit naturally ventilated hall can achieve thermal comfort in summer. - Abstract: Due to the strengthening of regulations and codes on building energy performance, as well as with the application of national legislations regarding energy management and efficiency, existing industrial facilities are using thermal refurbishment and renovation as impetus for increasing their overall energy efficiency. This paper analyzes a building envelope refurbishment for a case study of an existing historical industrial facility. Critical parameters affecting energy performance of industrial buildings were identified by reviewing relevant literate. Two retrofit scenarios were developed and dynamic thermal simulation using EnergyPlus was implemented to evaluate the potential for improvement. Thereby the impact of interior loads was considered, determined by measurements conducted on factory machines, occupancy and lighting operation patterns. However, information regarding constructions of the existing facility and installed technical building services is limited. There is also uncertainty in the quantification of natural ventilation air change rate for such buildings. To overcome these limitations a study of various material databases was carried out, in order to assess data for building envelope composition. Input values for missing data were provided based on literature, allowing a fair comparison between refurbishment alternatives. Simulation results showed that the heating demand of the facility could be reduced up to 52%, indicating a significant potential for energy savings. Beyond that, thermal performance against summer overheating also

  6. Fluorescence Spectra of Highlighter Inks

    Science.gov (United States)

    Birriel, Jennifer J.; King, Damon

    2018-01-01

    Fluorescence spectra excited by laser pointers have been the subject of several papers in "TPT". These papers all describe a fluorescence phenomenon in which the reflected laser light undergoes a change in color: this color change results from the combination of some partially reflected laser light and additional colors generated by…

  7. Labelling of living mammalian spermatozoa with the fluorescent thiol alkylating agent, monobromobimane (MB): immobilization upon exposure to ultraviolet light and analysis of acrosomal status

    Energy Technology Data Exchange (ETDEWEB)

    Cummins, J.M.; Fleming, A.D.; Crozet, N.; Kuehl, T.J.; Kosower, N.S.; Yanagimachi, R.

    1986-03-01

    Living spermatozoa of seven mammalian species were treated with the thiol-alkylating fluorescent labelling compound, monobromobimane (MBBR). MB-labelling alone had no effect on sperm motility, nor on the time course or ability of golden hamster spermatozoa to undergo the acrosome reaction when capacitated in vitro. Exposure of MB-labelled spermatozoa to ultraviolet (UV) light and excitation of the MB fluorochrome resulted in virtually immediate immobilization of the spermatozoa without affecting acrosomal status. UV exposure of unlabelled spermatozoa for up to 30 sec had no effect upon motility. Immobilization of MB-labelled spermatozoa depended on the midpiece being irradiated, as irradiation of the head alone, or of the more distal parts of the principal piece, had little or no effect upon motility. Labelling with MB followed by immobilization of individually selected spermatozoa was most useful for detailing the course and site of occurrence of the acrosome reaction during penetration of the cumulus oophorus by golden hamster spermatozoa in vitro. In these often hyperactivated spermatozoa, precise determination of the acrosomal status could not often otherwise be made due to the difficulty in visualizing the acrosomal region of a vigorously thrashing, hyperactivated spermatozoon. This technique should prove valuable in a variety of studies on sperm motility, capacitation and fertilization, and could also be extended to other cell systems.

  8. Synthesis of fluorescent C2-bridged teraryls and quateraryls for blue, sky-blue, and green color light-emitting devices.

    Science.gov (United States)

    Goel, Atul; Sharma, Ashutosh; Rawat, Madhu; Anand, R S; Kant, Ruchir

    2014-11-21

    A series of fluorescent teraryls and quateraryls were prepared from a ketene-S,S-acetal under mild conditions. These compounds exhibited blue, sky-blue and green color emissions both in the solid state and in a solution with good quantum yields, positive solvatochromic behavior, and reversible oxidation and reduction properties. The electronic characteristics of teraryl 6a and quateraryls 9a,b were examined by time-dependent density functional theory (TDDFT) calculations. Light-emitting devices were fabricated from teraryl 6a and quateraryls 9a,b as dyes and the configuration of ITO/PEDOT:PSS (40 nm)/NPB (20 nm)/ dye (50 nm)/BCP (7 nm)/ LiF (0.7 nm)/Al (200 nm), which exhibited electroluminescence maxima of 455, 480, and 525 nm, respectively. These devices operated at a substantially low turn-on voltage (3 and 4 V) and exhibited maximum luminance efficiencies of 0.62, 0.57, and 1.9 cd/A and brightnesses of 59, 160, and 1284 cd/m(2), respectively.

  9. Effect of an oral health education program based on the use of quantitative light-induced fluorescence technology in Uzbekistan adolescents.

    Science.gov (United States)

    Khudanov, Bakhtinur; Jung, Hoi In; Kahharova, Dono; Lee, Jeong-Woo; Hamidov, Ilhom; Lee, Eun-Song; Kim, Baek-Il

    2018-03-01

    The aim of this study was to determine whether an oral health education program using a Qscan device based on quantitative light-induced fluorescence (QLF) technology could improve the oral hygiene status and oral health literacy of adolescents. One hundred adolescents aged 14-16 years attending a school in Tashkent city were included in this study. The participants were assigned to the following two groups using permuted block randomization technique: (i) control group (traditional learning) and (ii) experimental group (Qscan device-based learning). The participants included in the experimental group received additional education and training on dental plaque removal using the Qscan device. The accumulated levels of plaque were assessed in all participants, who also completed questionnaires about their oral health status, oral health knowledge, attitude, and behavior during an 8-week period. There were statistically significant improvements in the experimental group compared to the control group in the plaque index (0.46 vs 0.07, p technology could be useful for improving the oral hygiene status and oral health literacy of adolescents in Uzbekistan. Copyright © 2018 Elsevier B.V. All rights reserved.

  10. Measure Guideline: Three High Performance Mineral Fiber Insulation Board Retrofit Solutions

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, Ken [Building Science Corporation, Westford, MA (United States)

    2015-01-01

    This Measure Guideline describes a high performance enclosure retrofit package that uses mineral fiber insulation board. The Measure Guideline describes retrofit assembly and details for wood frame roof and walls and for cast concrete foundations. This Measure Guideline is intended to serve contractors and designers seeking guidance for non-foam exterior insulation retrofit.

  11. Measure Guideline: Three High Performance Mineral Fiber Insulation Board Retrofit Solutions

    Energy Technology Data Exchange (ETDEWEB)

    Neuhauser, K. [Building Science Corporation, Westford, MA (United States)

    2015-01-01

    This Measure Guideline describes a high performance enclosure retrofit package that uses mineral fiber insulation board, and is intended to serve contractors and designers seeking guidance for non-foam exterior insulation retrofit processes. The guideline describes retrofit assembly and details for wood frame roof and walls and for cast concrete foundations.

  12. Field Assessment of Energy Audit Tools for Retrofit Programs

    Energy Technology Data Exchange (ETDEWEB)

    Edwards, J. [Univ. of Minnesota, St. Paul, MN (United States); Bohac, D. [Univ. of Minnesota, St. Paul, MN (United States); Nelson, C. [Univ. of Minnesota, St. Paul, MN (United States); Smith, I. [Univ. of Minnesota, St. Paul, MN (United States)

    2013-07-01

    This project focused on the use of home energy ratings as a tool to promote energy retrofits in existing homes. A home energy rating provides a quantitative appraisal of a home’s energy performance, usually compared to a benchmark such as the average energy use of similar homes in the same region. Rating systems based on energy performance models, the focus of this report, can establish a home’s achievable energy efficiency potential and provide a quantitative assessment of energy savings after retrofits are completed, although their accuracy needs to be verified by actual measurement or billing data. Ratings can also show homeowners where they stand compared to their neighbors, thus creating social pressure to conform to or surpass others. This project field-tested three different building performance models of varying complexity, in order to assess their value as rating systems in the context of a residential retrofit program: Home Energy Score, SIMPLE, and REM/Rate.

  13. Industrial-scale study of a retrofitted electrostatic precipitator

    Energy Technology Data Exchange (ETDEWEB)

    Jedrusik, M.; Jedrusik, J.; Sarna, M. [Technical University of Wroclaw, Wroclaw (Poland). Inst. of Heat Engineering and Fluid Mechanics

    1998-07-01

    Investigated was a retrofitted horizontal, three-zone electrostatic precipitator, which was designed in the late 1970s. The precipitator was installed after a bituminous-coal-fired 140 MW boiler. Prior to retrofit, the precipitator had spiked band corona electrodes which were to provide a firm efficiency of 95%. To make it comply with the enforced environmental standards it was necessary to increase this value. The retrofit consisted of replacing the spiked band corona electrode with a barbed-tube unit of a special design, which raised the efficiency of the precipitator to 99%, thus providing a dust concentration of 75 mg/m{sup 3} in the flue gas. These values were achieved without introducing additional zones. Prior to dust concentration measurements, the distribution of gas velocity at the inlet of an industrial precipitator was determined. The efficiency of the precipitator was measured for various heating power of the boiler to illustrate the improvement obtained. 6 refs., 3 figs., 2 tabs.

  14. Mini-Split Heat Pumps Multifamily Retrofit Feasibility Study

    Energy Technology Data Exchange (ETDEWEB)

    Dentz, J.; Podorson, D.; Varshney, K.

    2014-05-01

    Mini-split heat pumps can provide space heating and cooling in many climates and are relatively affordable. These and other features make them potentially suitable for retrofitting into multifamily buildings in cold climates to replace electric resistance heating or other outmoded heating systems. This report investigates the suitability of mini-split heat pumps for multifamily retrofits. Various technical and regulatory barriers are discussed and modeling was performed to compare long-term costs of substituting mini-splits for a variety of other heating and cooling options. A number of utility programs have retrofit mini-splits in both single family and multifamily residences. Two such multifamily programs are discussed in detail.

  15. Increasing draft capability for retrofit flue gas desulfurization systems

    International Nuclear Information System (INIS)

    Petersen, R.D.; Basel, B.E.; Mosier, R.J.

    1992-01-01

    The retrofit installation of flue gas desulfurization (FGD) systems results in significantly higher draft losses for existing generating stations. Consequently, the means for increasing draft capability must be included in many FGD retrofit projects. Consideration is given to several alternatives for increasing draft capability. Alternatives are developed for new induced draft (ID) fans to replace the existing ID fans and for new booster fans to supplement the existing ID fans. Both centrifugal and axial fans are evaluated, as are different means of fan volume control. Each alternative is evaluated on the basis of technical merit and economics. Presented are the development of fan alternatives and results of the technical and economic evaluations

  16. Comparison of the rate constants for energy transfer in the light-harvesting protein, C-phycocyanin, calculated from Foerster`s theory and experimentally measured by time-resolved fluorescence spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Debreczeny, Martin Paul [Univ. of California, Berkeley, CA (United States)

    1994-05-01

    We have measured and assigned rate constants for energy transfer between chromophores in the light-harvesting protein C-phycocyanin (PC), in the monomeric and trimeric aggregation states, isolated from Synechococcus sp. PCC 7002. In order to compare the measured rate constants with those predicted by Fdrster`s theory of inductive resonance in the weak coupling limit, we have experimentally resolved several properties of the three chromophore types ({beta}{sub 155} {alpha}{sub 84}, {beta}{sub 84}) found in PC monomers, including absorption and fluorescence spectra, extinction coefficients, fluorescence quantum yields, and fluorescence lifetimes. The cpcB/C155S mutant, whose PC is missing the {beta}{sub 155} chromophore, was, useful in effecting the resolution of the chromophore properties and in assigning the experimentally observed rate constants for energy transfer to specific pathways.

  17. A multi-criteria model for the comparison of building envelope energy retrofits

    Science.gov (United States)

    Donnarumma, Giuseppe; Fiore, Pierfrancesco

    2017-02-01

    In light of the current EU guidelines in the energy field, improving building envelope performance cannot be separated from the context of satisfying the environmental sustainability requirements, reducing the costs associated with the life cycle of the building as well as economic and financial feasibility. Therefore, identifying the "optimal" energy retrofit solutions requires the simultaneous assessment of several factors and thus becomes a problem of choice between several possible alternatives. To facilitate the work of the decision-makers, public or private, adequate decision support tools are of great importance. Starting from this need, a model based on the multi-criteria analysis "AHP" technique is proposed, along with the definition of three synthetic indices associated with the three requirements of "Energy Performance", "Sustainability Performance" and "Cost". From the weighted aggregation of the three indices, a global index of preference is obtained that allows to "quantify" the satisfaction level of the i-th alternative from the point of view of a particular group of decision-makers. The model is then applied, by way of example, to the case-study of the energetic redevelopment of a former factory, assuming its functional conversion. Twenty possible alternative interventions on the opaque vertical closures, resulting from the combination of three thermal insulators families (synthetic, natural and mineral) with four energy retrofitting techniques are compared and the results obtained critically discussed by considering the point of view of the three different groups of decision-makers.

  18. Evaluation of CNT Energy Savers Retrofit Packages Implemented in Multifamily Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Farley, Jenne [Partnership for Advanced Residential Retrofit (PARR), Chicago, IL (United States); Ruch, Russell [Partnership for Advanced Residential Retrofit (PARR), Chicago, IL (United States)

    2013-09-01

    This evaluation explored the feasibility of designing prescriptive retrofit measure packages for typical Chicago region multifamily buildings in order to achieve 25%-30% source energy savings through the study of three case studies. There is an urgent need to scale up energy efficiency retrofitting of Chicago's multifamily buildings in order to address rising energy costs and a rapidly depletingrental stock. Aimed at retrofit program administrators and building science professionals, this research project investigates the possibility of using prescriptive retrofit packages as a time- and resource-effective approach to the process of retrofitting multifamily buildings.

  19. Evaluation of CNT Energy Savers Retrofit Packages Implemented in Multifamily Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Farley, Jenne [Partnership for Advanced Residential Retrofit (PARR), Chicago, IL (United States); Ruch, Russell [Partnership for Advanced Residential Retrofit (PARR), Chicago, IL (United States)

    2013-09-01

    This evaluation explored the feasibility of designing prescriptive retrofit measure packages for typical Chicago region multifamily buildings in order to achieve 25%-30% source energy savings through the study of three case studies. There is an urgent need to scale up energy efficiency retrofitting of Chicago's multifamily buildings in order to address rising energy costs and a rapidly depleting rental stock. Aimed at retrofit program administrators and building science professionals, this research project investigates the possibility of using prescriptive retrofit packages as a time- and resource-effective approach to the process of retrofitting multifamily buildings.

  20. Public participation in energy saving retrofitting of residential buildings in China

    International Nuclear Information System (INIS)

    Liu, Wenling; Zhang, Jinyun; Bluemling, Bettina; Mol, Arthur P.J.; Wang, Can

    2015-01-01

    Highlights: • We compare public participation in three early cases of residential retrofitting in Beijing. • Residents’ involvement in pre-retrofit activities as well as in the choice and use of technologies varied. • More involvement of residents during retrofitting improves energy saving performance. • Taking into account motives and energy use practices of residents improves energy saving through retrofitting. - Abstract: Retrofitting existing residential buildings has been claimed as one crucial way to reduce energy consumption and greenhouse gas emissions within the Chinese residential sector. In China’s government-dominated retrofitting projects, the participation of residents is often neglected. The objective of this paper is to assess the influence level of public participation (before, during and after retrofit) on energy saving by comparing three Beijing neighborhoods with different retrofitting models: a central government-led model, a local government-led model, and an old neighborhood retrofit model. In the three cases data were collected through interviews with neighborhood workers and residents. The results show that residents’ involvement in pre-retrofit activities, in technology selection and in the use of technology differs greatly among the three cases. This study concludes that in order to improve the effectiveness of energy saving interventions, the motives, intentions and living habits of residents need to be given more consideration when designing and implementing retrofitting. By highlighting the importance of public participation this paper contributes to energy saving policy development in China

  1. Estimating Diurnal Courses of Gross Primary Production for Maize: A Comparison of Sun-Induced Chlorophyll Fluorescence, Light-Use Efficiency and Process-Based Models

    Directory of Open Access Journals (Sweden)

    Tianxiang Cui

    2017-12-01

    Full Text Available Accurately quantifying gross primary production (GPP is of vital importance to understanding the global carbon cycle. Light-use efficiency (LUE models and process-based models have been widely used to estimate GPP at different spatial and temporal scales. However, large uncertainties remain in quantifying GPP, especially for croplands. Recently, remote measurements of solar-induced chlorophyll fluorescence (SIF have provided a new perspective to assess actual levels of plant photosynthesis. In the presented study, we evaluated the performance of three approaches, including the LUE-based multi-source data synergized quantitative (MuSyQ GPP algorithm, the process-based boreal ecosystem productivity simulator (BEPS model, and the SIF-based statistical model, in estimating the diurnal courses of GPP at a maize site in Zhangye, China. A field campaign was conducted to acquire synchronous far-red SIF (SIF760 observations and flux tower-based GPP measurements. Our results showed that both SIF760 and GPP were linearly correlated with APAR, and the SIF760-GPP relationship was adequately characterized using a linear function. The evaluation of the modeled GPP against the GPP measured from the tower demonstrated that all three approaches provided reasonable estimates, with R2 values of 0.702, 0.867, and 0.667 and RMSE values of 0.247, 0.153, and 0.236 mg m−2 s−1 for the MuSyQ-GPP, BEPS and SIF models, respectively. This study indicated that the BEPS model simulated the GPP best due to its efficiency in describing the underlying physiological processes of sunlit and shaded leaves. The MuSyQ-GPP model was limited by its simplification of some critical ecological processes and its weakness in characterizing the contribution of shaded leaves. The SIF760-based model demonstrated a relatively limited accuracy but showed its potential in modeling GPP without dependency on climate inputs in short-term studies.

  2. New Benzimidazole-Based Bipolar Hosts: Highly Efficient Phosphorescent and Thermally Activated Delayed Fluorescent Organic Light-Emitting Diodes Employing the Same Device Structure.

    Science.gov (United States)

    Zhao, Yali; Wu, Chao; Qiu, Panlong; Li, Xiaoping; Wang, Qiang; Chen, Jiangshan; Ma, Dongge

    2016-02-03

    The rapid development of the thermally activated delayed fluorescence (TADF) emitters makes it necessary to produce new versatile host materials for both phosphorescent and TADF emitters. Three new bipolar host materials, 9,9'-(2'-(1H-benzimidazol-1-yl)-[1,1'-biphenyl]-3,5-diyl)bis(9H-carbazole) (o-mCPBI), 9,9'-(3'-(1H-benzimidazol-1-yl)-[1,1'-biphenyl]-3,5-diyl)bis(9H-carbazole) (m-mCPBI), and 9,9'-(4'-(1H-benzimidazol-1-yl)-[1,1'-biphenyl]-3,5-diyl)bis(9H-carbazole) (p-mCPBI), are designed and synthesized by integrating mCP with benzimidazole moiety via the ortho-, meta-, and para-positions of N-phenyl. The influence of different linking modes on the thermal, photophysical, electrochemical, and charge transport properties of the compounds is studied. By employing the same device structure except the emitting layer, the device performances of the blue, green, yellow, red, white phosphorescent and blue, green TADF organic light-emitting diodes (OLEDs) based on the three hosts are investigated. Among these three hosts, o-mCPBI exhibits the best device performance with external quantum efficiencies of over 20% for phosphorescent OLEDs and enhanced efficiencies for TADF devices. All these devices show relatively low efficiency roll-offs at high brightness. The versatility of the benzimidazole-based bipolar host o-mCPBI, such as an extremely high triplet energy level, suitable molecular orbital energy levels, improved thermal and charge transport properties, and excellent device performances for various colors, makes it a universal host material for highly efficient both phosphorescent and TADF OLEDs.

  3. A new light on Alkaptonuria: A Fourier-transform infrared microscopy (FTIRM) and low energy X-ray fluorescence (LEXRF) microscopy correlative study on a rare disease.

    Science.gov (United States)

    Mitri, Elisa; Millucci, Lia; Merolle, Lucia; Bernardini, Giulia; Vaccari, Lisa; Gianoncelli, Alessandra; Santucci, Annalisa

    2017-05-01

    Alkaptonuria (AKU) is an ultra-rare disease associated to the lack of an enzyme involved in tyrosine catabolism. This deficiency results in the accumulation of homogentisic acid (HGA) in the form of ochronotic pigment in joint cartilage, leading to a severe arthropathy. Secondary amyloidosis has been also unequivocally assessed as a comorbidity of AKU arthropathy. Composition of ochronotic pigment and how it is structurally related to amyloid is still unknown. We exploited Synchrotron Radiation Infrared and X-Ray Fluorescence microscopies in combination with conventional bio-assays and analytical tools to characterize chemical composition and morphology of AKU cartilage. We evinced that AKU cartilage is characterized by proteoglycans depletion, increased Sodium levels, accumulation of lipids in the peri-lacunar regions and amyloid formation. We also highlighted an increase of aromatic compounds and oxygen-containing species, depletion in overall Magnesium content (although localized in the peri-lacunar region) and the presence of calcium carbonate fragments in proximity of cartilage lacunae. We highlighted common features between AKU and arthropathy, but also specific signatures of the disease, like presence of amyloids and peculiar calcifications. Our analyses provide a unified picture of AKU cartilage, shedding a new light on the disease and opening new perspectives. Ochronotic pigment is a hallmark of AKU and responsible of tissue degeneration. Conventional bio-assays have not yet clarified its composition and its structural relationship with amyloids. The present work proposes new strategies for filling the aforementioned gap that encompass the integration of new analytical approaches with standardized analyses. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Dowel Bar Retrofit Mix Design and Specification : Technical Report

    Science.gov (United States)

    2012-01-01

    Current INDOT specifications for repair materials to be used in dowel bar retrofit (DBR) applications (Sections 507.08 and 901.07 of INDOTs Book of Specifications) are based, in large part, on the requirements of ASTM C 928 and the manufacturer-pr...

  5. Indoor Environmental Quality Benefits of Apartment Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Noris, Federico [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Adamkiewicz, Gary [Harvard School of Public Health, Boston MA (United States); Delp, William W. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Hotchi, Toshifumi [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Russell, Marion [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Singer, Brett C. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Spears, Michael [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Vermeer, Kimberly [Urban Habitate Initiatives Inc., Boston, MA (United States); Fisk, William J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2013-06-01

    Sixteen apartments serving low-income populations in three buildings were retrofit with the goal of simultaneously reducing energy consumption and improving indoor environmental quality (IEQ). Retrofit measures varied among apartments and included, among others, envelope sealing, installation of continuous mechanical ventilation systems, upgrading bathroom fans and range hoods, attic insulation, replacement of heating and cooling systems, and adding wall-mounted particle air cleaners. IEQ parameters were measured, generally for two one-week periods before and after the retrofits. The measurements indicate an overall improvement in IEQ conditions after the retrofits. Comfort conditions, bathroom humidity, and concentrations of carbon dioxide, acetaldehyde, volatile organic compounds, and particles generally improved. Formaldehyde and nitrogen dioxide levels decreased in the building with the highest concentrations, were unchanged in a second building, and increased in a third building. IEQ parameters other than particles improved more in apartments with continuous mechanical ventilation systems installed. In general, but not consistently, larger percent increases in air exchange rates were associated with larger percent decreases in indoor levels of the pollutants that primarily come from indoor sources.

  6. The application, benefits and challenges of retrofitting the existing buildings

    Science.gov (United States)

    Khairi, Muhammad; Jaapar, Aini; Yahya, Zaharah

    2017-11-01

    Sustainable development has been the main topic of debate for years in some countries such as United Kingdom, United State of America and Malaysia. Depletion of natural resources, global warming, economics uncertainty and health issues are some of the reasons behind sustainable development movements, it is not just a political debate in the parliament but more towards collective works among sectors in order to minimizing the negative impact of development to the environment and other living organism. Retrofit an existing building is one of the solutions to reduce the dependency on constructing new buildings. There are huge numbers of existing building stocks that suitable to be retrofitted such as historical buildings, offices, residential, warehouse, factories, vacant buildings and other historical buildings. Therefore, the aim of this research is to provide information on the application, benefits and challenges of retrofitting an existing building. Two buildings were chosen as case studies following by site visits and observation to the buildings. The data were then compared in a table form. Primary and secondary sources were also used for this research. The application of retrofit should be promoted across the construction and conservation industries since it has significant tangible and intangible benefits. It is one of the most environmentally friendly and efficient solutions to optimize the energy performance and could also helps to extend the life of the existing building or historical buildings while ensuring optimum thermal comfort for the occupants which leads to higher productivity.

  7. Optimal Retrofit Scheme for Highway Network under Seismic Hazards

    Directory of Open Access Journals (Sweden)

    Yongxi Huang

    2014-06-01

    Full Text Available Many older highway bridges in the United States (US are inadequate for seismic loads and could be severely damaged or collapsed in a relatively small earthquake. According to the most recent American Society of Civil Engineers’ infrastructure report card, one-third of the bridges in the US are rated as structurally deficient and many of these structurally deficient bridges are located in seismic zones. To improve this situation, at-risk bridges must be identified and evaluated and effective retrofitting programs should be in place to reduce their seismic vulnerabilities. In this study, a new retrofit strategy decision scheme for highway bridges under seismic hazards is developed and seamlessly integrate the scenario-based seismic analysis of bridges and the traffic network into the proposed optimization modeling framework. A full spectrum of bridge retrofit strategies is considered based on explicit structural assessment for each seismic damage state. As an empirical case study, the proposed retrofit strategy decision scheme is utilized to evaluate the bridge network in one of the active seismic zones in the US, Charleston, South Carolina. The developed modeling framework, on average, will help increase network throughput traffic capacity by 45% with a cost increase of only $15million for the Mw 5.5 event and increase the capacity fourfold with a cost of only $32m for the Mw 7.0 event.

  8. A structured approach to heat exchanger network retrofit design

    NARCIS (Netherlands)

    Van Reisen, J.L.B.

    2008-01-01

    Process plants have high energy consumption. Much energy can be saved by a proper design of the heat exchanger network, which contains the main heat transferring equipment of the plant. Existing plants can often be made more energy-efficient by a retrofit: the (physical) modification of the

  9. Closed cycle MHD power plant and retrofit optimization application

    Science.gov (United States)

    Cutting, J. C.; Owens, W. R.; Sheth, P. R.; Griswold, J.; Wehrey, M.

    1980-06-01

    The results of two independent studies of closed-cycle MHD power systems are presented. A combined cycle consisting of an MHD closed-cycle topping unit retrofitted to an existing steam bottoming plant is considered. Preliminary results of an ongoing parametric study of an MHD closed-cycle system utilizing an integrated pressurized coal gasifier are discussed.

  10. Public Housing: A Tailored Approach to Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Dentz, J. [Advanced Residential Integrated Energy Solutions Collaborative (ARIES), New York, NY (United States); Conlin, F. [Advanced Residential Integrated Energy Solutions Collaborative (ARIES), New York, NY (United States); Podorson, D. [Advanced Residential Integrated Energy Solutions Collaborative (ARIES), New York, NY (United States); Alaigh, K. [Advanced Residential Integrated Energy Solutions Collaborative (ARIES), New York, NY (United States); Davis, T. [Advanced Residential Integrated Energy Solutions Collaborative (ARIES), New York, NY (United States)

    2016-02-23

    The Building America research team Advanced Residential Integrated Energy Solutions Collaborative (ARIES) worked with four public housing authorities (PHAs) to develop packages of energy-efficiency retrofit measures that the PHAs can cost-effectively implement with their own staffs during the normal course of housing operations when units are refurbished between occupancies.

  11. Whole Foods Market Retrofits Multiple Building Systems for Big Savings

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-03-01

    Whole Foods Market partnered with U.S. the Department of Energy (DOE) to develop and implement solutions to reduce annual energy consumption in existing stores by at least 30% versus pre-retrofit energy use at its store in Edgewater, New Jersey, as part of DOE’s Commercial Building Partnership (CBP) program.

  12. High power solid state retrofit lamp thermal characterization and modeling

    NARCIS (Netherlands)

    Jakovenko, J.; Formánek, J.; Vladimír, J.; Husák, M.; Werkhoven, R.J.

    2012-01-01

    Thermal and thermo-mechanical modeling and characterization of solid state lightening (SSL) retrofit LED Lamp are presented in this paper. Paramount Importance is to design SSL lamps for reliability, in which thermal and thermo-mechanical aspects are key points. The main goal is to get a precise 3D

  13. Stoke's and anti-Stoke's characteristics of anaerobic and aerobic bacterias at excitation of fluorescence by low-intensity red light: I. Research of anaerobic bacterias

    Science.gov (United States)

    Masychev, Victor I.; Alexandrov, Michail T.

    2000-04-01

    Biopsy or photo dynamic therapy of tumors are usually investigated by fluorescent diagnostics methods. Information on modified method of fluorescence diagnostics of inflammatory diseases is represented in this research. Anaerobic micro organisms are often the cause of these pathological processes. These micro organisms also accompany disbiotic processes in intestines.

  14. Multi-criteria decision model for retrofitting existing buildings

    Directory of Open Access Journals (Sweden)

    M. D. Bostenaru Dan

    2004-01-01

    Full Text Available Decision is an element in the risk management process. In this paper the way how science can help in decision making and implementation for retrofitting buildings in earthquake prone urban areas is investigated. In such interventions actors from various spheres are involved. Their interests range among minimising the intervention for maximal preservation or increasing it for seismic safety. Research was conducted to see how to facilitate collaboration between these actors. A particular attention was given to the role of time in actors' preferences. For this reason, on decision level, both the processural and the personal dimension of risk management, the later seen as a task, were considered. A systematic approach was employed to determine the functional structure of a participative decision model. Three layers on which actors implied in this multi-criteria decision problem interact were identified: town, building and element. So-called 'retrofit elements' are characteristic bearers in the architectural survey, engineering simulations, costs estimation and define the realms perceived by the inhabitants. This way they represent an interaction basis for the interest groups considered in a deeper study. Such orientation means for actors' interaction were designed on other levels of intervention as well. Finally, an 'experiment' for the implementation of the decision model is presented: a strategic plan for an urban intervention towards reduction of earthquake hazard impact through retrofitting. A systematic approach proves thus to be a very good communication basis among the participants in the seismic risk management process. Nevertheless, it can only be applied in later phases (decision, implementation, control only, since it serves verifying and improving solution and not developing the concept. The 'retrofit elements' are a typical example of the detailing degree reached in the retrofit design plans in these phases.

  15. Decision making based on analysis of benefit versus costs of preventive retrofit versus costs of repair after earthquake hazards

    Science.gov (United States)

    Bostenaru Dan, M.

    2012-04-01

    dissipaters) to different amount and location in the building was considered. Device computations, a civil engineering method for building economics (and which was, before statistics existed, also the method for computing the costs of general upgrade of buildings), were done for the retrofit and for the repair measures, being able to be applied for different countries, also ones where there is no database on existing projects in seismic retrofit. The building elements for which the device computations were done are named "retrofit elements" and they can be new elements, modified elements or replaced elements of the initial building. The addition of the devices is simple, as the row in project management was, but, for the sake of comparison, also complex project management computed in other works was compared for innovative measures such as FRP (with glass and fibre). The theoretical costs for model measures were compared to the way costs of real retrofit for this building type (with reinforced concrete jacketing and FRP) are computed in Greece. The theoretical proposed measures were generally compared to those applied in practice, in Romania and Italy as well. A further study will include these, as in Italy diagonal braces with dissipation had been used. The typology of braces is relevant also for the local seismic culture, maybe outgoing for another type of skeleton structures the distribution of which has been studied: the timber skeleton. A subtype of Romanian reinforced concrete skeleton buildings includes diagonal braces. In order to assess the costs of rebuilding or general upgrade without retrofit, architecture methods for building economics are considered based on floor surface. Diagrams have been built to see how the total costs vary as addition between the preventive retrofit and the post-earthquake repair, and tables to compare to the costs of rebuilding, outgoing from a the model of addition of day-lighting in atria of buildings. The moment when a repair measure

  16. Measurement and Verification of Energy Savings and Performance from Advanced Lighting Controls

    Energy Technology Data Exchange (ETDEWEB)

    PNNL

    2016-02-21

    This document provides a framework for measurement and verification (M&V) of energy savings, performance, and user satisfaction from lighting retrofit projects involving occupancy-sensor-based, daylighting, and/or other types of automatic lighting. It was developed to provide site owners, contractors, and other involved organizations with the essential elements of a robust M&V plan for retrofit projects and to assist in developing specific project M&V plans.

  17. Concentrators using fluorescent substances

    Energy Technology Data Exchange (ETDEWEB)

    Hayashibara, M.; Tsukamoto, M. (Hitachi Seisakusho K.K., Tokyo (Japan))

    1990-01-01

    In luminescent concentrators - plates of polymethylmethacrylate (PMMA) or other transparent material with a fluorescent compound dispersed within them - incident light is trapped and concentrated by internal reflection, and shifted to a longer wavelength, as it interacts with fluorescent particles. Experience with the use of luminescent concentrators for electricity generation in conjunction with solar cells, in solar heaters, in amplifiers for light intensity, in long-wave converters and in display panels is discussed. Solar energy conversion efficiencies of 4-5% have been obtained in generating systems combining concentrators containing Fluorol 555 or Rhodamin 6G with GaAs solar cells. (author).

  18. Fluorescence uranium determination

    International Nuclear Information System (INIS)

    Fernandez Cellini, R.; Crus Castillo, F. de la; Barrera Pinero, R.

    1960-01-01

    An equipment for analysis of uranium by fluorescence was developed in order to determine it at such a low concentration that it can not be determined by the most sensible analytical methods. this new fluorimeter was adapted to measure the fluorescence emitted by the phosphorus sodium fluoride-sodium carbonate-potasium carbonate-uranyl, being excited by ultraviolet light of 3,650 A the intensity of the light emitted was measure with a photomultiplicator RCA 5819 and the adequate electronic equipment. (Author) 19 refs

  19. Magnetic and fluorescent carbon-based nanohybrids for multi-modal imaging and magnetic field/NIR light responsive drug carriers.

    Science.gov (United States)

    Wang, Hui; Zhou, Shuiqin

    2016-07-21

    Carbon nanomaterials have gained significant momentum as promising candidate materials for biomedical applications due to their unique structure and properties. After functionalization with magnetic and fluorescent components, the resultant carbon-based nanohybrids can serve not only as magnetic resonance and fluorescence imaging contrast agents, but also as photothermally/magneto-thermally responsive drug carriers for combined photothermo/chemotherapy. This mini-review summarizes the latest developments and applications and addresses the future perspectives of carbon-based magnetic and fluorescent nanohybrids in the biomedical field.

  20. Flexible transparent aerogels as window retrofitting films and optical elements with tunable birefringence

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Qingkun; Frazier, Allister W.; Zhao, Xinpeng; De La Cruz, Joshua A.; Hess, Andrew J.; Yang, Ronggui; Smalyukh, Ivan I.

    2018-06-01

    Experimental realization of optically transparent, mechanically robust and flexible aerogels has been a longstanding challenge, which limits their practical applications in energy-saving devices, such as thermally insulating films for enhancing energy efficiency of windows. The poor transparency precluded even hypothetical consideration of the possibility of birefringent aerogels. We develop birefringent and optically isotropic aerogels that combine properties of thermal super-insulation, mechanical robustness and flexibility, and transparency to visible-spectrum light. This unusual combination of physical properties is achieved by combining liquid crystalline self-organization of cellulose nanofibers with polysiloxane cross-linking and control of the nanoscale porosity to form hybrid organic-inorganic mesostructured aerogels. Potential applications of these inexpensive materials range from single pane window retrofitting to smart fabrics.

  1. LED Shipboard Lighting: A Comparative Analysis

    Science.gov (United States)

    2009-12-01

    viability of retrofitting the Navy fleet with newer lighting technology . C. RESEARCH QUESTION The research project provided to NPS by Dr. Larry Schuette...LED is a key component in today’s lighting technology . Modern households use LEDs in such components as digital video disc, (DVD) readers...manufactures that the Navy is serious in implementing lighting technology with a multi- year demonstration seeing the benefits provided by the manufactures

  2. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2016-01-01

    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses the foundati......Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  3. Resource handbook for low-income residential retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Callaway, J.W.; Brenchley, D.L.; Davis, L.J.; Ivey, D.L.; Smith, S.A.; Westergard, E.J.

    1987-04-01

    The purpose of the handbook is to provide technical assistance to state grantees participating in the Partnerships in Low-Income Residential Retrofit (PILIRR) Program. PILIRR is a demonstration program aimed at identifying innovative, successful approaches to developing public and private support for weatherization of low-income households. The program reflects the basic concept that responsibility for financial support for conservation activities such as low-income residential retrofitting is likely to gradually shift from the DOE to the states and the private sector. In preparing the handbook, PNL staff surveyed over 50 programs that provide assistance to low-income residents. The survey provided information on factors that contribute to successful programs. PNL also studied the winning PILIRR proposals (from the states of Florida, Iowa, Kentucky, Oklahoma and Washington) and identified the approaches proposed and the type of information that would be most helpful in implementing these approaches.

  4. Evolution of the CANDU control centre retrofit and new stations

    International Nuclear Information System (INIS)

    Olmstead, R.A.; Mitchell, W.

    1991-01-01

    Significant event data from operating nuclear plants in many countries consistently indicates human errors are the root cause for 40-60% of operating station significant events. Because so much information is already in digital form, opportunities exist to improve the CANDU control centre with retrofits that exploit this information. These opportunities are enhanced because of rapid technological development in computers and electronics, coupled with significant progress in the behavioural sciences that greatly increases our knowledge of the cognitive strengths and weaknesses of human beings. CANDU control rooms are undergoing retrofits and for future CANDU stations, a new concept of the control centre is emerging. The objective is to significantly reduce the incidence of human error, reduce operations and maintenance costs and improve both reliability and safety

  5. Cascade Apartments - Deep Energy Multifamily Retrofit , Kent, Washington (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    None, None

    2014-02-01

    In December of 2009-10, King County Housing Authority (KCHA) implemented energy retrofit improvements in the Cascade multifamily community, located in Kent, Washington (marine climate.)This research effort involved significant coordination from stakeholders KCHA, WA State Department of Commerce, utility Puget Sound Energy, and Cascade tenants. This report focuses on the following three primary BA research questions : 1. What are the modeled energy savings using DOE low income weatherization approved TREAT software? 2. How did the modeled energy savings compare with measured energy savings from aggregate utility billing analysis? 3. What is the Savings to Investment Ratio (SIR) of the retrofit package after considering utility window incentives and KCHA capitol improvement funding.

  6. A Wireless Platform for Energy Efficient Building Control Retrofits

    Science.gov (United States)

    2012-07-01

    equipment condition; (iii) building size (7000 sq. ft. space) and usage (mixed with offices , conference rooms, cubicles and a cafeteria having different...IL) was demonstrated. The medium-size office building underwent a retrofit of the HVAC system and controls employing a technology called optimal...gratefully acknowledge the financial support and technical guidance provided by the SERDP/ESTCP Office under the leadership of leadership Drs. Jeff

  7. Energy Efficient Crawlspace Foundation Retrofit: Mixed Humid Climate

    Energy Technology Data Exchange (ETDEWEB)

    Del Bianco, M.; Wiehagen, J.; Wood, A.

    2013-01-01

    Residential quality management systems have most often been designed for new home construction. To address quality in existing homes in the form of Scopes of Work (SOW), the NAHB Research Center began with a new construction scope of work and applied it to an existing home project. This document is intended to outline the steps of translating a new home construction SOW to SOW for retrofit.

  8. Energy Efficient Crawlspace Foundation Retrofit: Mixed Humid Climate

    Energy Technology Data Exchange (ETDEWEB)

    Del Bianco, M. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States); Wiehagen, J. [NAHB Research Center Industry Partnership, Upper Marlboro, MD (United States)

    2013-01-01

    Residential quality management systems have most often been designed for new home construction. To address quality in existing homes in the form of Scopes of Work (SOW), the NAHB Research Center began with a new construction scope of work and applied it to an existing home project. This document is intended to outline the steps of translating a new home construction SOW to SOW for retrofit and addressed crawlspace foundations in a mixed-humid climate.

  9. A Proposed Retrofit for Bridges Subjected to Wave Forces

    OpenAIRE

    Govan, Paul

    2010-01-01

    Damage due to recent hurricanes along the Gulf Coast of Mexico publicized the need for research of engineering practices in coastal areas. Specifically, many bridges near the coast were not designed for storm surge and wind induced wave loads. Many of these damaged bridges are being rebuilt higher with correspondingly higher costs. The following proposal concerns using existing seismic retrofitting techniques for simply supported bridges as a solution to this problem. The proposed connection ...

  10. Next Step Toward Widespread Residential Deep Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    McIlvaine, J.; Saunders, S.; Bordelon, E.; Baden, S.; Elam, L.; Martin, E.

    2013-07-01

    The complexity of deep energy retrofits warrants additional training to successfully manage multiple improvements that will change whole house air, heat, and moisture flow dynamics. The home performance contracting industry has responded to these challenges by aggregating skilled labor for assessment of and implementation under one umbrella. Two emerging business models are profiled that seek to resolve many of the challenges, weaknesses, opportunities, and threats described for the conventional business models.

  11. The Next Step Toward Widespread Residential Deep Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    McIlvaine, J. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States); Martin, E. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States); Saunders, S. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States); Bordelon, E. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States); Baden, S. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States); Elam, L. [Building American Partnership for Residential Construction (BA-PIRC), Cocoa, FL (United States)

    2013-07-01

    The complexity of deep energy retrofits warrants additional training to successfully manage multiple improvements that will change whole house air, heat, and moisture flow dynamics. The home performance contracting industry has responded to these challenges by aggregating skilled labor for assessment of and implementation under one umbrella. Two emerging business models are profiled that seek to resolve many of the challenges, weaknesses, opportunities, and threats described for the conventional business models.

  12. Retrofitted gravity mediation without the gravitino-overproduction problem

    International Nuclear Information System (INIS)

    Endo, M.; Takahashi, F.; Yanagida, T.T.; Tokyo Univ.

    2007-02-01

    We propose a retrofitted gravity mediation model which alleviates the gravitino overproduction from decays of an inflaton and a supersymmetry breaking field. In the model, we introduce an approximate U(1) symmetry under which the supersymmetry breaking field is charged, although it is broken by a mass term of messenger fields to generate gaugino masses of order the weak scale. In a low-scale inflation model, we find regions in which the gravitino overproduction problem is avoided. (orig.)

  13. Naturally occurring fluorescence in frogs.

    Science.gov (United States)

    Taboada, Carlos; Brunetti, Andrés E; Pedron, Federico N; Carnevale Neto, Fausto; Estrin, Darío A; Bari, Sara E; Chemes, Lucía B; Peporine Lopes, Norberto; Lagorio, María G; Faivovich, Julián

    2017-04-04

    Fluorescence, the absorption of short-wavelength electromagnetic radiation reemitted at longer wavelengths, has been suggested to play several biological roles in metazoans. This phenomenon is uncommon in tetrapods, being restricted mostly to parrots and marine turtles. We report fluorescence in amphibians, in the tree frog Hypsiboas punctatus, showing that fluorescence in living frogs is produced by a combination of lymph and glandular emission, with pigmentary cell filtering in the skin. The chemical origin of fluorescence was traced to a class of fluorescent compounds derived from dihydroisoquinolinone, here named hyloins. We show that fluorescence contributes 18-29% of the total emerging light under twilight and nocturnal scenarios, largely enhancing brightness of the individuals and matching the sensitivity of night vision in amphibians. These results introduce an unprecedented source of pigmentation in amphibians and highlight the potential relevance of fluorescence in visual perception in terrestrial environments.

  14. Interaction of poxvirus intracellular mature virion proteins with the TPR domain of kinesin light chain in live infected cells revealed by two-photon-induced fluorescence resonance energy transfer fluorescence lifetime imaging microscopy.

    Science.gov (United States)

    Jeshtadi, Ananya; Burgos, Pierre; Stubbs, Christopher D; Parker, Anthony W; King, Linda A; Skinner, Michael A; Botchway, Stanley W

    2010-12-01

    Using two-photon-induced fluorescence lifetime imaging microscopy, we corroborate an interaction (previously demonstrated by yeast two-hybrid domain analysis) of full-length vaccinia virus (VACV; an orthopoxvirus) A36 protein with the cellular microtubule motor protein kinesin. Quenching of enhanced green fluorescent protein (EGFP), fused to the C terminus of VACV A36, by monomeric red fluorescent protein (mDsRed), fused to the tetratricopeptide repeat (TPR) domain of kinesin, was observed in live chicken embryo fibroblasts infected with either modified vaccinia virus Ankara (MVA) or wild-type fowlpox virus (FWPV; an avipoxvirus), and the excited-state fluorescence lifetime of EGFP was reduced from 2.5 ± 0.1 ns to 2.1 ± 0.1 ns due to resonance energy transfer to mDsRed. FWPV does not encode an equivalent of intracellular enveloped virion surface protein A36, yet it is likely that this virus too must interact with kinesin to facilitate intracellular virion transport. To investigate possible interactions between innate FWPV proteins and kinesin, recombinant FWPVs expressing EGFP fused to the N termini of FWPV structural proteins Fpv140, Fpv168, Fpv191, and Fpv198 (equivalent to VACV H3, A4, p4c, and A34, respectively) were generated. EGFP fusions of intracellular mature virion (IMV) surface protein Fpv140 and type II membrane protein Fpv198 were quenched by mDsRed-TPR in recombinant FWPV-infected cells, indicating that these virion proteins are found within 10 nm of mDsRed-TPR. In contrast, and as expected, EGFP fusions of the IMV core protein Fpv168 did not show any quenching. Interestingly, the p4c-like protein Fpv191, which demonstrates late association with preassembled IMV, also did not show any quenching.

  15. Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles

    Czech Academy of Sciences Publication Activity Database

    Humpolíčková, J.; Procházka, K.; Hof, Martin; Tuzar, Zdeněk; Špírková, Milena

    2003-01-01

    Roč. 19, - (2003), s. 4111-4119 ISSN 0743-7463 R&D Projects: GA MŠk LN00A032; GA ČR GA203/01/0536; GA ČR GA203/01/0735 Institutional research plan: CEZ:AV0Z4050913; CEZ:AV0Z4040901 Keywords : fluorescence * spectroscopy Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.098, year: 2003

  16. Chamberlain Heights Redevelopment: A Large Scale, Cold Climate Study of Affordable Housing Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Donnelly, K. [Consortium for Advanced Residential Buildings (CARB), Norwalk, CT (United States); Mahle, M. [Consortium for Advanced Residential Buildings (CARB), Norwalk, CT (United States)

    2012-03-01

    The City of Meriden Housing Authority (MHA) collaborated with affordable housing developer Jonathon Rose Companies (JRC) to complete a gut renovation of 124 residential units in the Chamberlain Heights retrofit project. The affordable housing community is made up of 36 buildings in duplex and quad configurations located on 22 acres within two miles of downtown Meriden, CT. The final post-retrofit analysis showed 40-45% source energy savings over the existing pre-retrofit conditions.

  17. Byggmeister Test Home. Analysis and Initial Results of Cold Climate Wood-Framed Home Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    Gates, C. [Building Science Corporation (BSC), Somerville, MA (United States)

    2013-01-01

    BSC seeks to further the energy efficiency market for New England area retrofit projects by supporting projects that are based on solid building science fundamentals that will benefit the homeowner through a combination of energy savings, improved durability, and occupant comfort. This report describes a deep retrofit project of a two-family wood-framed home in Belmont, Massachusetts, and examines the retrofit measures for the enclosure amd mechanical systems and reviews the decision-making process that took place during planning.

  18. Thermo economical evaluation of retrofitting strategies in air conditioning systems

    Energy Technology Data Exchange (ETDEWEB)

    Tribess, Arlindo; Fiorelli, Flavio Augusto Sanzogo; Hernandez Neto, Alberto [Sao Paulo Univ., SP (Brazil). Escola Politecnica. Dept. de Engenharia Mecanica]. E-mail: atribess@usp.br; fiorelli@usp.br; ahneto@usp.br

    2000-07-01

    In a building project, several subsystems are designed, among them the air conditioning system. Electrical energy consumption profiles show that this subsystem is responsible for 40 to 50% of total consumption in a commercial building. Besides the study of technical aspects that should be considered in order to assure the thermal comfort of the occupants as well the temperature and humidity conditions for an efficient equipment operation, an economical evaluation of this subsystem should be also made. In retrofit projects, the economical aspect is also critical for such projects in order to assure bigger efficiency in an economically attractive way. This paper analyses some strategies that might be adopted in retrofitting an air conditioning system installed in a commercial building with mixed occupation. By mixed we mean that some floors have a typical office occupation profile and other floors are mainly occupied by electronic equipment. This analysis includes both technical and economical evaluation. The proposed solutions performance are compared to the old system, which allows to verify the retrofitting impact in energy consumption reduction and its economical feasibility. (author)

  19. Field Assessment of Energy Audit Tools for Retrofit Programs

    Energy Technology Data Exchange (ETDEWEB)

    Edwards, J.; Bohac, D.; Nelson, C.; Smith, I.

    2013-07-01

    This project focused on the use of home energy ratings as a tool to promote energy retrofits in existing homes. A home energy rating provides a quantitative appraisal of a home's asset performance, usually compared to a benchmark such as the average energy use of similar homes in the same region. Home rating systems can help motivate homeowners in several ways. Ratings can clearly communicate a home's achievable energy efficiency potential, provide a quantitative assessment of energy savings after retrofits are completed, and show homeowners how they rate compared to their neighbors, thus creating an incentive to conform to a social standard. An important consideration is how rating tools for the retrofit market will integrate with existing home energy service programs. For residential programs that target energy savings only, home visits should be focused on key efficiency measures for that home. In order to gain wide adoption, a rating tool must be easily integrated into the field process, demonstrate consistency and reasonable accuracy to earn the trust of home energy technicians, and have a low monetary cost and time hurdle for homeowners. Along with the Home Energy Score, this project also evaluated the energy modeling performance of SIMPLE and REM/Rate.

  20. Deep Energy Retrofit Performance Metric Comparison: Eight California Case Studies

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Fisher, Jeremy [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Less, Brennan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2014-06-01

    In this paper we will present the results of monitored annual energy use data from eight residential Deep Energy Retrofit (DER) case studies using a variety of performance metrics. For each home, the details of the retrofits were analyzed, diagnostic tests to characterize the home were performed and the homes were monitored for total and individual end-use energy consumption for approximately one year. Annual performance in site and source energy, as well as carbon dioxide equivalent (CO2e) emissions were determined on a per house, per person and per square foot basis to examine the sensitivity to these different metrics. All eight DERs showed consistent success in achieving substantial site energy and CO2e reductions, but some projects achieved very little, if any source energy reduction. This problem emerged in those homes that switched from natural gas to electricity for heating and hot water, resulting in energy consumption dominated by electricity use. This demonstrates the crucial importance of selecting an appropriate metric to be used in guiding retrofit decisions. Also, due to the dynamic nature of DERs, with changes in occupancy, size, layout, and comfort, several performance metrics might be necessary to understand a project’s success.

  1. Move over! Stock turnover, retrofit and industrial energy efficiency

    International Nuclear Information System (INIS)

    Worrell, Ernst; Biermans, Gijs

    2005-01-01

    We demonstrate the importance of stock turnover on industrial energy efficiency through a literature review and a case study of energy-intensive equipment, i.e. the electric arc furnace in the US steel industry. We describe the common methods for assessing stock turnover. We have found that both stock turnover and retrofit are important elements to explain the energy efficiency improvement rates. We investigated the development of electricity use in electric arc furnaces in the United States by tracking the development of individual furnaces over the period 1990-2002. This provides a detailed picture of changes in the stock or fleet of furnaces through turnover and/or retrofit. Our results confirm the results of other empirical studies that there is no clear lifetime of equipment. However, retired furnaces are distinctly less efficient than furnaces remaining in the stock, while new furnaces are distinctly more efficient than the average stock. We found an annual average improvement in specific electricity consumption of 1.3%/year over the studied period, of which 0.7%/year was due to stock turnover and 0.5%/year due to retrofit of stock in service throughout the period

  2. Experiment study on RC frame retrofitted by the external structure

    Science.gov (United States)

    Liu, Chunyang; Shi, Junji; Hiroshi, Kuramoto; Taguchi, Takashi; Kamiya, Takashi

    2016-09-01

    A new retrofitting method is proposed herein for reinforced concrete (RC) structures through attachment of an external structure. The external structure consists of a fiber concrete encased steel frame, connection slab and transverse beams. The external structure is connected to the existing structure through a connection slab and transverse beams. Pseudostatic experiments were carried out on one unretrofitted specimen and three retrofitted frame specimens. The characteristics, including failure mode, crack pattern, hysteresis loops behavior, relationship of strain and displacement of the concrete slab, are demonstrated. The results show that the load carrying capacity is obviously increased, and the extension length of the slab and the number of columns within the external frame are important influence factors on the working performance of the existing structure. In addition, the displacement difference between the existing structure and the outer structure was caused mainly by three factors: shear deformation of the slab, extraction of transverse beams, and drift of the conjunction part between the slab and the existing frame. Furthermore, the total deformation determined by the first two factors accounted for approximately 80% of the damage, therefore these factors should be carefully considered in engineering practice to enhance the effects of this new retrofitting method.

  3. Sourcing of Steam and Electricity for Carbon Capture Retrofits.

    Science.gov (United States)

    Supekar, Sarang D; Skerlos, Steven J

    2017-11-07

    This paper compares different steam and electricity sources for carbon capture and sequestration (CCS) retrofits of pulverized coal (PC) and natural gas combined cycle (NGCC) power plants. Analytical expressions for the thermal efficiency of these power plants are derived under 16 different CCS retrofit scenarios for the purpose of illustrating their environmental and economic characteristics. The scenarios emerge from combinations of steam and electricity sources, fuel used in each source, steam generation equipment and process details, and the extent of CO 2 capture. Comparing these scenarios reveals distinct trade-offs between thermal efficiency, net power output, levelized cost, profit, and net CO 2 reduction. Despite causing the highest loss in useful power output, bleeding steam and extracting electric power from the main power plant to meet the CCS plant's electricity and steam demand maximizes plant efficiency and profit while minimizing emissions and levelized cost when wholesale electricity prices are below 4.5 and 5.2 US¢/kWh for PC-CCS and NGCC-CCS plants, respectively. At prices higher than these higher profits for operating CCS retrofits can be obtained by meeting 100% of the CCS plant's electric power demand using an auxiliary natural gas turbine-based combined heat and power plant.

  4. An Experimental and Analytical Evaluation of Wall And Window Retrofit Configurations: Supporting the Residential Retrofit Best Practices Guide

    Energy Technology Data Exchange (ETDEWEB)

    Stovall, Therese K [ORNL; Petrie, Thomas [ORNL; Kosny, Jan [ORNL; Childs, Phillip W [ORNL; Atchley, Jerald Allen [ORNL; Hulvey, Kimberly D [ORNL

    2007-11-01

    A Retrofit Best Practices Guide was developed to encourage homeowners to consider energy conservation issues whenever they modify their siding or windows. In support of this guide, an experimental program was implemented to measure the performance of a number of possible wall siding and window retrofit configurations. Both thermal and air-leakage measurements were made for a 2.4 x 2.4 m (8 x 8 ft) wall section with and without a 0.9 x 1.2 m (3 x 4 ft) window. The windows tested were previously well-characterized at a dedicated window test facility. A computer model was also used to provide information for the Best Practices Guide. The experimental data for walls and windows were used in conjunction with this model to estimate the total annual energy savings for several typical houses in a number of different locations.

  5. Protocol for Maximizing Energy Savings and Indoor Environmental Quality Improvements when Retrofiting Apartments

    Energy Technology Data Exchange (ETDEWEB)

    Noris, Federico [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Delp, William W. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Vermeer, Kimberly [Urban Habitat Initiatives Inc., Boston, MA (United States); Adamkiewicz, Gary [Harvard School of Public Health, Boston, MA (United States); Singer, Brett C. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Fisk, William J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2012-06-18

    The current focus on building energy retrofit provides an opportunity to simultaneously improve indoor environmental quality (IEQ). Toward this end, we developed a protocol for selecting packages of retrofits that both save energy and improve IEQ in apartments. The protocol specifies the methodology for selecting retrofits from a candidate list while addressing expected energy savings, IEQ impacts, and costs in an integrated manner. Interviews, inspections and measurements are specified to collect the needed input information. The protocol was applied to 17 apartments in three buildings in two different climates within California. Diagnostic measurements and surveys conducted before and after retrofit implementation indicate enhanced apartment performance.

  6. Protocol for maximizing energy savings and indoor environmental quality improvements when retrofitting apartments

    Energy Technology Data Exchange (ETDEWEB)

    Noris, Federico [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Delp, William W. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Vermeer, Kimberly [Urban Habitat Initiatives Inc., Boston, MA (United States); Adamkiewicz, Gary [Harvard School of Public Health, Boston, MA (United States); Singer, Brett C. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Fisk, William J. [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2013-06-01

    The current focus on building energy retrofit provides an opportunity to simultaneously improve indoor environmental quality (IEQ). Toward this end, we developed a protocol for selecting packages of retrofits that both save energy and improve IEQ in apartments. The protocol specifies the methodology for selecting retrofits from a candidate list while addressing expected energy savings, IEQ impacts, and costs in an integrated manner. Interviews, inspections and measurements are specified to collect the needed input information. The protocol was applied to 17 apartments in three buildings in two different climates within California. Diagnostic measurements and surveys conducted before and after retrofit implementation indicate enhanced apartment performance.

  7. Temperature effects on microalgal photosynthesis-light responses measured by O-2 production, pulse-amplitude-modulated fluorescence, and C-14 assimilation

    DEFF Research Database (Denmark)

    Hancke, Kasper; Hancke, Torunn B.; Olsen, Lasse M.

    2008-01-01

    Short-term temperature effects on photosynthesis were investigated by measuring O-2 production, PSII-fluorescence kinetics, and C-14-incorporation rates in monocultures of the marine phytoplankton species Prorocentrum minimum (Pavill.) J. Schiller (Dinophyceae), Prymnesium parvum f. patelliferum ...... to the absorption properties in PSII.......Short-term temperature effects on photosynthesis were investigated by measuring O-2 production, PSII-fluorescence kinetics, and C-14-incorporation rates in monocultures of the marine phytoplankton species Prorocentrum minimum (Pavill.) J. Schiller (Dinophyceae), Prymnesium parvum f. patelliferum (J...

  8. The effect of light and temperature on the dynamic state of Rhodobacter sphaeroides reaction centers proteins determined from changes in tryptophan fluorescence lifetime and P+QA-recombination kinetics.

    Science.gov (United States)

    Knox, Peter P; Gorokhov, Vladimir V; Korvatovskiy, Boris N; Lukashev, Eugene P; Goryachev, Sergey N; Paschenko, Vladimir Z; Rubin, Andrew B

    2018-02-06

    The temperature dependencies of the rate of dark recombination of separated charges between the photoactive bacteriochlorophyll and the primary quinone acceptor (Q A ) in photosynthetic reaction centers (RCs) of the purple bacteria Rhodobacter sphaeroides (Rb. sphaeroides) were investigated. Measurements were performed in water-glycerol and trehalose environments after freezing to -180 °C in the dark and under actinic light with subsequent heating. Simultaneously, the RC tryptophanyl fluorescence lifetime in the spectral range between 323 and 348 nm was measured under these conditions. A correlation was found between the temperature dependencies of the functional and dynamic parameters of RCs in different solvent mixtures. For the first time, differences in the average fluorescence lifetime of tryptophanyl residues were measured between RCs frozen in the dark and in the actinic light. The obtained results can be explained by the RC transitions between different conformational states and the dynamic processes in the structure of the hydrogen bonds of RCs. We assumed that RCs exist in two main microconformations - "fast" and "slow", which are characterized by different rates of P + and Q A - recombination reactions. The "fast" conformation is induced in frozen RCs in the dark, while the "slow" conformation of RC occurs when the RC preparation is frozen under actinic light. An explanation of the temperature dependencies of tryptophan fluorescence lifetimes in RC proteins was made under the assumption that temperature changes affect mainly the electron transfer from the indole ring of the tryptophan molecule to the nearest amide or carboxyl groups. Copyright © 2018. Published by Elsevier B.V.

  9. Building America Case Study: Retrofitting Garden-Style Apartments, Brooklyn, New York

    Energy Technology Data Exchange (ETDEWEB)

    2016-12-01

    This research effort sought to achieve a solution package that yields energy savings greater than 30 percent over the pre-existing conditions in a minimally intrusive multifamily retrofit project. The Consortium for Advanced Residential Buildings (CARB) partnered with L+M Development Partners, Inc. on a Mitchell-Lama Housing Program project, Marcus Garvey Village, in Brooklyn, NY (Climate Zone 4A). The Mitchell-Lama Housing Program is a form of housing subsidy in the state of New York that provides affordable rental and cooperative housing to moderate- and middle-income families. Marcus Garvey Village was founded in 1975 and contains 625 residential units (ranging from studios to 5-bedroom units) in thirty-two 4-story garden-style apartment structures built with concrete and faced in light brown brick. The single largest challenge for implementation of energy conservation measures at Marcus Garvey was working within occupied spaces. Measures are being implemented in phases to minimize disruption. As of August 2015, the retrofit work is over 50 percent complete. The wall insulation, sealing of the through wall AC vent, and installation of new oil-filled electric baseboards with advanced controls are conducted at one time, limiting disruption to the living room space. In a similar fashion, the kitchen work is done, then the bathroom. The final selection of energy conservation measures is projected to save 26.5 percent in source energy with a cost just under $3.7 million and utility bill savings of nearly $480,000 (of an average $1.8 million annual utility cost for the development).

  10. Simulation of PSII-operating efficiency from chlorophyll fluorescence in response to light and temperature in chrysanthemum (Dendranthema grandiflora) using a multilayer leaf model

    DEFF Research Database (Denmark)

    Janka, E.; Körner, O.; Rosenqvist, Eva

    2018-01-01

    Chlorophyll fluorescence serves as a proxy photosynthesis measure under different climatic conditions. The objective of the study was to predict PSII quantum yield using greenhouse microclimate data to monitor plant conditions under various climates. Multilayer leaf model was applied to model...

  11. Retrofitting Of RCC Piles By Using Basalt Fiber Reinforced Polymer BFRP Composite Part 1 Review Papers On RCC Structures And Piles Retrofitting Works.

    Directory of Open Access Journals (Sweden)

    R. Ananda Kumar

    2015-01-01

    Full Text Available Abstract Retrofitting works are immensely essential for deteriorated and damaged structures in Engineering and Medical fields in order to keep or return to the originality for safe guarding the structures and consumers. In this paper different types of methods of retrofitting review notes are given based on the experimental numerical and analytical methods results on strengthening the Reinforced cement concrete RCC structures including RCC piles. Soil-pile interaction on axial load lateral load reviews are also presented. This review paper is prepared to find out the performance of basalt fibre reinforced polymer BFRP composite retrofitted reinforced cement concrete single end bearing piles.

  12. Demonstrating Fluorescence with Neon Paper and Plastic

    Science.gov (United States)

    Birriel, Jennifer J.; Roe, Clarissa

    2015-01-01

    Several papers in this journal have dealt with the fluorescence in orange neon plastic, olive oil, and soda. In each case, the fluorescent emission was excited by either green or violet-blue laser light. In this paper, we examine the fluorescent emission spectra of so-called neon colored papers and plastic clipboards available in department and…

  13. Low-E Retrofit Demonstration and Educational Program

    Energy Technology Data Exchange (ETDEWEB)

    Culp, Thomas D [Birch Point Consulting LLC; Wiehagen, Joseph [Home Innovation Research Labs, Inc.; Drumheller, S Craig [Home Innovation Research Labs, Inc.; Siegel, John [Quanta Technologies Inc.; Stratmoen, Todd [Larson Manufacturing

    2013-11-16

    The objective of this project was to demonstrate the capability of low-emissivity (low-E) storm windows / panels and low-E retrofit glazing systems to significantly and cost effectively improve the energy efficiency of both existing residential and commercial buildings. The key outcomes are listed below: RESIDENTIAL CASE STUDIES: (a) A residential case study in two large multifamily apartment buildings in Philadelphia showed a substantial 18-22% reduction in heating energy use and a 9% reduction in cooling energy use by replacing old clear glass storm windows with modern low-E storm windows. Furthermore, the new low-E storm windows reduced the overall apartment air leakage by an average of 10%. (b) Air leakage testing on interior low-E panels installed in a New York City multifamily building over windows with and without AC units showed that the effective leakage area of the windows was reduced by 77-95%. (c) To study the use of low-E storm windows in a warmer mixed climate with a balance of both heating and cooling, 10 older homes near Atlanta with single pane windows were tested with three types of exterior storm windows: clear glass, low-E glass with high solar heat gain, and low-E glass with lower solar heat gain. The storm windows significantly reduced the overall home air leakage by an average of 17%, or 3.7 ACH50. Considerably high variability in the data made it difficult to draw strong conclusions about the overall energy usage, but for heating periods, the low-E storm windows showed approximately 15% heating energy savings, whereas clear storm windows were neutral in performance. For cooling periods, the low-E storm windows showed a wide range of performance from 2% to over 30% cooling energy savings. Overall, the study showed the potential for significantly more energy savings from using low-E glass versus no storm window or clear glass storm windows in warmer mixed climates, but it is difficult to conclusively say whether one type of low-E performed

  14. An Assessment of Envelope Measures in Mild Climate Deep Energy Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Walker, Iain [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Less, Brennan [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2014-06-01

    Energy end-uses and interior comfort conditions have been monitored in 11 Deep Energy Retrofits (DERs) in a mild marine climate. Two broad categories of DER envelope were identified: first, bringing homes up to current code levels of insulation and airtightness, and second, enhanced retrofits that go beyond these code requirements. The efficacy of envelope measures in DERs was difficult to determine, due to the intermingled effects of enclosure improvements, HVAC system upgrades and changes in interior comfort conditions. While energy reductions in these project homes could not be assigned to specific improvements, the combined effects of changes in enclosure, HVAC system and comfort led to average heating energy reductions of 76percent (12,937 kWh) in the five DERs with pre-retrofit data, or 80percent (5.9 kWh/ft2) when normalized by floor area. Overall, net-site energy reductions averaged 58percent (15,966 kWh; n=5), and DERs with code-style envelopes achieved average net-site energy reductions of 65percent (18,923 kWh; n=4). In some homes, the heating energy reductions were actually larger than the whole house reductions that were achieved, which suggests that substantial additional energy uses were added to the home during the retrofit that offset some heating savings. Heating system operation and energy use was shown to vary inconsistently with outdoor conditions, suggesting that most DERs were not thermostatically controlled and that occupants were engaged in managing the indoor environmental conditions. Indoor temperatures maintained in these DERs were highly variable, and no project home consistently provided conditions within the ASHRAE Standard 55-2010 heating season comfort zone. Thermal comfort and heating system operation had a large impact on performance and were found to depend upon the occupant activities, so DERs should be designed with the occupants needs and patterns of consumption in mind. Beyond-code building envelopes were not found to be

  15. Tenants' experiences and satisfaction in social housing subject to comprehensive retrofitting

    DEFF Research Database (Denmark)

    Knudsen, Henrik Nellemose; Thomsen, Kirsten Engelund; Rose, Jørgen

    This report describes a Danish retrofitting project with focus on reducing the energy consump-tion in social housing. The objective of the project was to study tenants’ overall satisfaction with how the retrofitting of their dwellings was carried out and their experiences and satisfaction...

  16. Sonoma House: Monitoring of the First U.S. Passive House Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    German, A.; Weitzel, B.; Backman, C.; Hoeschele, M.; Dakin, B.

    2012-12-01

    The Sonoma Deep Retrofit is a single-story deep retrofit project in the marine climate of Sonoma, California. The design was guided by Passive House principles which promote the use of very high levels of wall, ceiling, and floor insulation along with tight envelope construction to maintain a comfortable indoor environment with little or no need for conventional heating or cooling.

  17. Sonoma House. Monitoring of the First U.S. Passive House Retrofit

    Energy Technology Data Exchange (ETDEWEB)

    German, A. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Weitzel, B. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Backman, C. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Hoeschele, M. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States); Dakin, B. [Alliance for Residential Building Innovation (ARBI), Davis, CA (United States)

    2012-12-01

    The Sonoma Deep Retrofit is a single-story deep retrofit project in the marine climate of Sonoma, California. The design was guided by Passive House principles that promote the use of very high levels of wall, ceiling, and floor insulation along with tight envelope construction to maintain a comfortable indoor environment with little or no need for conventional heating or cooling.

  18. Retrofitting of homes for people in fuel poverty: Approach based on household thermal comfort

    International Nuclear Information System (INIS)

    Vilches, Alberto; Barrios Padura, Ángela; Molina Huelva, Marta

    2017-01-01

    In a context of fuel poverty, the application of cost-effective methodology for energy retrofitting of buildings is ineffective. In these situations, there is no energy consumption reduction and thermal comfort is hardly achieved. This paper introduces a methodology to choose the most appropriate retrofit measure in a context of fuel poverty. This methodology is based on thermal comfort as the main criterion, and secondarily, it is based on the budget for paying monthly energy bills and initial costs. This study demonstrates how thermal comfort and monthly available income of households must be the first criterion for decision-making process. The methodology has been applied in four buildings. Results demonstrate why active retrofit measures are ineffective when monthly budget to pay energy bills is reduced. In conclusion, recommendations are made to promote efficient public policies in energy retrofit. The study was supported by the European Regional Development Fund (ERDF) and carried out within the research project ‘{Re} Programa. (Re)habitation +(Re)generation +(Re)programming’ during 2013–2015. - Highlights: • A methodology is proposed for energy retrofit in a fuel poverty context. • Householder's monthly budget determines the energy retrofit effectiveness. • The cost-effective methodology is not applicable in a fuel poverty context. • Energy retrofit does not reduce energy consumption in a fuel poverty context. • Energy retrofit does not produce monthly monetary savings in a fuel poverty context.

  19. 40 CFR 1043.50 - Approval of methods to meet Tier 1 retrofit NOX standards.

    Science.gov (United States)

    2010-07-01

    ... retrofit NOX standards. 1043.50 Section 1043.50 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF NOX, SOX, AND PM EMISSIONS FROM MARINE ENGINES AND VESSELS SUBJECT TO THE MARPOL PROTOCOL § 1043.50 Approval of methods to meet Tier 1 retrofit NOX standards...

  20. Field testing of a low-cost retrofit filter berm to treat stormwater runoff contaminants.

    Science.gov (United States)

    2008-09-01

    The goal of this cooperative effort between MaineDOT and the University of New Hampshire was to test a low-cost : retrofit filter berm that would reduce non-point pollution from highway runoff. The retrofit berm would be easy to : construct using rea...