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Sample records for fluorescence fish dans

  1. Application of fluorescence in situ hybridization (FISH) to the ...

    African Journals Online (AJOL)

    Application of fluorescence in situ hybridization (FISH) to the analysis of ... In this study, fluorescent in situ hybridization (FISH) as a culture-independent molecular ... a high percentage and took place in an oily biological system under aerobic ...

  2. PENGARUH JENIS DAN KONSENTRASI ASAM, TEMPERATUR DAN WAKTU EKSTRAKSI TERHADAP KARAKTERISTIK FISH GLUE DARI LIMBAH IKAN TENGGIRI

    Directory of Open Access Journals (Sweden)

    Tony Handoko

    2012-05-01

    Full Text Available THE EFFECTS OF TYPES AND ACID CONCENTRATIONS, TEMPERATURES AND EXTRACTION TIME ON THE FISH GLUE CHARACTERISTIC OBTAINED FROM MACKEREL FISH BONE WASTE. As a maritime nation, Indonesia produced fresh fish products up to 4,408,419 tons in 2005. Mackerel fish is one of them. Its bone waste has an economic value as a source for fish glue production. The purpose of this research was to determine the optimum conditions in fish glue processing. Preliminary research was done to determine the acid type (CH3COOH and HCl and its concentration (4%, 5%, and 6% in soaking process. While the main research was then done to determine the best temperature (45oC, 60oC, and 75oC and time of extraction (4 hrs, 5 hrs, 6 hrs. The fish glue products were analyzed their adhesion and physical characteristics, such as density, viscosity, pH, and water content. The results showed that weak acid (CH3COOH of 5% concentration is the best solution in soaking process and extraction in 4 hrs at 45oC has given the optimum condition for producing fish glue. The glue has a nice odor but its adhesion strength remains poor.      Abstrak   Sebagai negara maritim, Indonesia menghasilkan produk perikanan yang sangat besar mencapai 4.408.419 ton pada tahun 2005 dan terus bertambah. Salah satu produk perikanan yang terbesar adalah ikan tenggiri dengan limbah tulang yang mempunyai nilai ekonomis yang tinggi sebagai bahan baku fish glue. Penelitian ini bertujuan untuk menemukan kondisi proses yang dapat menghasilkan fish glue yang baik melalui dua tahap yaitu penelitian pendahuluan dan utama. Penelitian pendahuluan bertujuan mendapatkan jenis asam (CH3COOH dan HCl dan konsentrasi asam terbaik (4%, 5%, dan 6% pada  proses perendaman tulang ikan. Penelitian utama bertujuan  menentukan temperatur ekstraksi (45oC, 60oC, dan 75oC dan waktu ekstraksi (4 jam, 5 jam, 6 jam. Analisis fish glue yang dilakukan adalah uji kerekatan dan sifat fisik berupa densitas, viskositas, pH, dan kadar air

  3. Red fluorescence in reef fish: A novel signalling mechanism?

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    Siebeck Ulrike E

    2008-09-01

    Full Text Available Abstract Background At depths below 10 m, reefs are dominated by blue-green light because seawater selectively absorbs the longer, 'red' wavelengths beyond 600 nm from the downwelling sunlight. Consequently, the visual pigments of many reef fish are matched to shorter wavelengths, which are transmitted better by water. Combining the typically poor long-wavelength sensitivity of fish eyes with the presumed lack of ambient red light, red light is currently considered irrelevant for reef fish. However, previous studies ignore the fact that several marine organisms, including deep sea fish, produce their own red luminescence and are capable of seeing it. Results We here report that at least 32 reef fishes from 16 genera and 5 families show pronounced red fluorescence under natural, daytime conditions at depths where downwelling red light is virtually absent. Fluorescence was confirmed by extensive spectrometry in the laboratory. In most cases peak emission was around 600 nm and fluorescence was associated with guanine crystals, which thus far were known for their light reflecting properties only. Our data indicate that red fluorescence may function in a context of intraspecific communication. Fluorescence patterns were typically associated with the eyes or the head, varying substantially even between species of the same genus. Moreover red fluorescence was particularly strong in fins that are involved in intraspecific signalling. Finally, microspectrometry in one fluorescent goby, Eviota pellucida, showed a long-wave sensitivity that overlapped with its own red fluorescence, indicating that this species is capable of seeing its own fluorescence. Conclusion We show that red fluorescence is widespread among marine fishes. Many features indicate that it is used as a private communication mechanism in small, benthic, pair- or group-living fishes. Many of these species show quite cryptic colouration in other parts of the visible spectrum. High inter

  4. A fluorescent chromatophore changes the level of fluorescence in a reef fish.

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    Matthias F Wucherer

    Full Text Available Body coloration plays a major role in fish ecology and is predominantly generated using two principles: a absorbance combined with reflection of the incoming light in pigment colors and b scatter, refraction, diffraction and interference in structural colors. Poikilotherms, and especially fishes possess several cell types, so-called chromatophores, which employ either of these principles. Together, they generate the dynamic, multi-color patterns used in communication and camouflage. Several chromatophore types possess motile organelles, which enable rapid changes in coloration. Recently, we described red fluorescence in a number of marine fish and argued that it may be used for private communication in an environment devoid of red. Here, we describe the discovery of a chromatophore in fishes that regulates the distribution of fluorescent pigments in parts of the skin. These cells have a dendritic shape and contain motile fluorescent particles. We show experimentally that the fluorescent particles can be aggregated or dispersed through hormonal and nervous control. This is the first description of a stable and natural cytoskeleton-related fluorescence control mechanism in vertebrate cells. Its nervous control supports suggestions that fluorescence could act as a context-dependent signal in some marine fish species and encourages further research in this field. The fluorescent substance is stable under different chemical conditions and shows no discernible bleaching under strong, constant illumination.

  5. A simple protocol for attenuating the auto-fluorescence of cyanobacteria for optimized fluorescence in situ hybridization (FISH) imaging.

    Science.gov (United States)

    Zeller, Perrine; Ploux, Olivier; Méjean, Annick

    2016-03-01

    Cyanobacteria contain pigments, which generate auto-fluorescence that interferes with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce the auto-fluorescence of Microcystis strains. These protocols were successfully applied in FISH experiments using 16S rRNA specific probes and filamentous cyanobacteria.

  6. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus)

    OpenAIRE

    Raden Roro Sri Pudji Sinarni Dewi; Alimuddin Alimuddin; Agus Oman Sudrajat; Komar Sumantadinata; Erma Primanita Hayuningtyas

    2016-01-01

    Penelitian ekspresi sementara (transient expression) dari transgen secara in vivo menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein). Pengamatan gen EGFP (enhanced green fluorescent protein) pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus) ditujukan untuk mendapatkan informasi mengenai kema...

  7. Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish

    Science.gov (United States)

    Hardison, D. Ransom; Holland, William C.; McCall, Jennifer R.; Bourdelais, Andrea J.; Baden, Daniel G.; Darius, H. Taiana; Chinain, Mireille; Tester, Patricia A.; Shea, Damian; Flores Quintana, Harold A.; Morris, James A.; Litaker, R. Wayne

    2016-01-01

    Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R)). However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA(R) in certain labs. A fluorescence based receptor binding assay (RBA(F)) was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY®- PbTx-2) for voltage-gated sodium channel receptors at site 5 instead of a radiolabeled brevetoxin. Responses were linear in fish tissues spiked from 0.1 to 1.0 ppb with Pacific ciguatoxin-3C (P-CTX-3C) with a detection limit of 0.075 ppb. Carribean ciguatoxins were confirmed in Caribbean fish by LC-MS/MS analysis of the regional biomarker (C-CTX-1). Fish (N = 61) of six different species were screened using the RBA(F). Results for corresponding samples analyzed using the neuroblastoma cell-based assay (CBA-N2a) correlated well (R2 = 0.71) with those of the RBA(F), given the low levels of CTX present in positive fish. Data analyses also showed the resulting toxicity levels of P-CTX-3C equivalents determined by CBA-N2a were consistently lower than the RBA(F) affinities expressed as % binding equivalents, indicating that a given amount of toxin bound to the site 5 receptors translates into corresponding lower cytotoxicity. Consequently, the RBA(F), which takes approximately two hours to perform, provides a generous estimate relative to the widely used CBA-N2a which requires 2.5 days to complete. Other RBA(F) advantages include the long-term (> 5 years) stability of the BODIPY®- PbTx-2 and having similar results as the commonly used RBA(R). The RBA(F) is cost-effective, allows high sample

  8. Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish.

    Science.gov (United States)

    Hardison, D Ransom; Holland, William C; McCall, Jennifer R; Bourdelais, Andrea J; Baden, Daniel G; Darius, H Taiana; Chinain, Mireille; Tester, Patricia A; Shea, Damian; Quintana, Harold A Flores; Morris, James A; Litaker, R Wayne

    2016-01-01

    Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R)). However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA(R) in certain labs. A fluorescence based receptor binding assay (RBA(F)) was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY®-PbTx-2) for voltage-gated sodium channel receptors at site 5 instead of a radiolabeled brevetoxin. Responses were linear in fish tissues spiked from 0.1 to 1.0 ppb with Pacific ciguatoxin-3C (P-CTX-3C) with a detection limit of 0.075 ppb. Carribean ciguatoxins were confirmed in Caribbean fish by LC-MS/MS analysis of the regional biomarker (C-CTX-1). Fish (N = 61) of six different species were screened using the RBA(F). Results for corresponding samples analyzed using the neuroblastoma cell-based assay (CBA-N2a) correlated well (R2 = 0.71) with those of the RBA(F), given the low levels of CTX present in positive fish. Data analyses also showed the resulting toxicity levels of P-CTX-3C equivalents determined by CBA-N2a were consistently lower than the RBA(F) affinities expressed as % binding equivalents, indicating that a given amount of toxin bound to the site 5 receptors translates into corresponding lower cytotoxicity. Consequently, the RBA(F), which takes approximately two hours to perform, provides a generous estimate relative to the widely used CBA-N2a which requires 2.5 days to complete. Other RBA(F) advantages include the long-term (> 5 years) stability of the BODIPY®-PbTx-2 and having similar results as the commonly used RBA(R). The RBA(F) is cost-effective, allows high sample

  9. FLUORESCENT TRANSGENIC FISH IN PERU: BIOSAFETY AND RISK ANALYSIS PENDING

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    Scotto, Carlos

    2010-07-01

    Full Text Available Transgenesis involves processes of molecular genetic manipulation of DNAwhich seeks to "introduce genes" of interest from one organism into the genetic material of another to obtain goods or services. The resulting organism is called a Genetically Modified Organism or GMO. It shows the first case of transgenic fluorescent fish as a real example of GMOs existing in Peru. Reproduction and hybridization in confined environments, provide new approaches to biosecurity decision-makers about this new technological contribution to the task of Peru.

  10. Pengendalian Penyakit Layu Pisang dengan Fusarium Nonpatogenik dan Fluorescent pseudomonads

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    Christanti Sumardiyono

    2007-12-01

    Full Text Available The study was aimed to know the ability of fluorescent pseudomonads and nonpathogenic Fusarium for controlling fusarium wilt of banan. The research was conducted both in the laboratory and glass ouse in the Faculty of Agriculture Gadjah Mada University. Laboratorial trial incuded the examining of antagonistic capacity in vitro and the population of these two antagonistic microorganism in compost medium. The examination of the effect of these two microorganisms in compost medium against fusarium wilt of banana Ambon Kuning cultivar was conducted in a glass house. The result showed that there was no antagonistic mechanism occured between fluorescent pseudomonads and nonpathogenic Fusarium. The treatment of compost with fluorescent pseudomonads or/and nonpathogenic Fusarium showed that there were differences on the optimum population of these two antagonistic microorganisms. Fluorescent pseudomonads attained its optimum population one week after inoculation while nonpathogenic Fusarium attained its optimum population two weeks after inoculation. The glass house trial showed taht compost enriched with two microorganisms with one week inocubation time reduced percentage of wilted leaves, although it was nonsignificant with control treatment. Field experiment should be conducted with higher population density of antagonist microorganisms.

  11. POLA EKSPRESI GEN ENHANCED GREEN FLUORESCENT PROTEIN PADA EMBRIO DAN LARVA IKAN PATIN SIAM (Pangasianodon hypophthalmus

    Directory of Open Access Journals (Sweden)

    Raden Roro Sri Pudji Sinarni Dewi

    2016-04-01

    menggunakan gen reporter berguna untuk mendesain konstruksi gen yang akan digunakan pada penelitian transgenesis. Gen reporter yang umum digunakan dalam penelitian ekspresi sementara transgen adalah gen GFP (green fluorescent protein. Pengamatan gen EGFP (enhanced green fluorescent protein pada embrio dan larva ikan patin siam (Pangasianodon hypophthalmus ditujukan untuk mendapatkan informasi mengenai kemampuan promoter -aktin ikan mas dalam mengendalikan ekspresi gen EGFP. Gen EGFP diintroduksikan ke dalam sperma ikan patin siam menggunakan metode elektroporasi. Sperma yang telah dielektroporasi digunakan untuk membuahi sel telur ikan patin siam. Pengamatan ekspresi gen EGFP dilakukan setiap enam jam dimulai dari embrio fase 2 sel sampai larva. Berdasarkan hasil penelitian, gen EGFP terekspresi pada fase embrio dan larva ikan patin siam. Puncak ekspresi gen EGFP terjadi pada fase neurula dan menurun pada fase larva. Berdasarkan penelitian ini maka ikan patin siam transgenik telah berhasil dibentuk dan promoter -aktin ikan mas terbukti aktif dalam mengarahkan ekspresi gen asing (GFP di dalam tubuh ikan patin siam.

  12. MOTIVASI, SIKAP, DAN INTENSI PENGGUNA MEDIA SOSIAL PADA KAMPANYE STOP ILLEGAL FISHING

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    La Moriansyah

    2016-11-01

    Full Text Available Social media is commonly used to promote social campaigns, one of which is Stop Illegal Fishing campaign. To achieve success in this campaign, some conditions have to be fulfilled i.e. positive attitudes of the social media users toward the campaigns and high intentions to provide recommendations (word of mouth to others. This study was conducted to analyze types of motivations affecting attitudes of users towards social campaigns (stop illegal fishing in social media. In addition, it also analyzed the influence of attitudes on users’ intentions in performing WOM. The method utilized to meet the objectives was Partial Least Square. The results showed that users’ motivation in using social media and towards the campaign messages are perceived to have significantly a positive effect on the attitude towards social campaigns in social media. Besides, the increase in the intention to perform WOM is positively influenced by the attitudes of social media users. There are differences identified in the motivation that affect the attitudes of social media users towards campaign of the two groups i.e. the social media users who have never seen the Stop Illegal Fishing campaign (151 samples and those who have seen the campaign in social media.    Keywords:  motivation, attitude, word of mouth, user experience, uses and gratification theory, digital campaigns, social campaigns, stop illegal fishingABSTRAKMedia sosial sering digunakan untuk mempromosikan kampanye sosial, contohnya adalah  kampanye Stop Illegal Fishing. Untuk meraih kesuksesan pada kampanye ini, beberapa hal yang harus diraih adalah sikap positif pengguna media sosial terhadap kampanye dan intensi yang tinggi untuk memberikan rekomendasi (word of mouth kepada orang lain. Penelitian ini dilakukan untuk menganalisis motivasi pengguna apa saja yang memengaruhi sikap pengguna media sosial terhadap kampanye sosial (stop illegal fishing di media sosial. Selain itu menganalisis sikap

  13. FISH BILIARY POLYCYCLIC AROMATIC HYDROCARBON METABOLITES ESTIMATED BY FIXED-WAVELENGTH FLUORESCENCE: COMPARISON WITH HPLC-FLUORESCENT DETECTION

    Science.gov (United States)

    Fixed wavelength fluorescence (FF) was compared to high-performance liquid chromatography with fluorescence detection (HPLC-F) as an estimation of polycyclic aromatic hydrocarbon (PAH) exposure to fish. Two excitation/emission wavelength pairs were used to measure naphthalene- an...

  14. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

    DEFF Research Database (Denmark)

    Vedarethinam, Indumathi; Shah, Pranjul Jaykumar; Dimaki, Maria;

    2010-01-01

    Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the pr......Fluorescence in situ Hybridization (FISH) is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA...

  15. COmbined Binary RAtio fluorescence in situ hybridiziation (COBRA-FISH): development and applications.

    Science.gov (United States)

    Raap, A K; Tanke, H J

    2006-01-01

    The ability to probe for the location of DNA sequences in morphologically preserved chromosomes and nuclei by fluorescence in situ hybridization (FISH) provided for cytogenetics a quantum leap forward in resolution and ease of detection of chromosomal aberrations. COBRA-FISH, an acronym for COmbined Binary RAtio-FISH is a multicolor FISH methodology, which enables recognition of all human chromosome arms on the basis of color, thus greatly facilitating cytogenetic analysis. It also permits gene and viral integration site mapping in the context of chromosome arm painting. Here we review the principle, practice and applications of COBRA-FISH.

  16. Red fluorescence increases with depth in reef fishes, supporting a visual function, not UV protection.

    Science.gov (United States)

    Meadows, Melissa G; Anthes, Nils; Dangelmayer, Sandra; Alwany, Magdy A; Gerlach, Tobias; Schulte, Gregor; Sprenger, Dennis; Theobald, Jennifer; Michiels, Nico K

    2014-09-07

    Why do some marine fishes exhibit striking patterns of natural red fluorescence? In this study, we contrast two non-exclusive hypotheses: (i) that UV absorption by fluorescent pigments offers significant photoprotection in shallow water, where UV irradiance is strongest; and (ii) that red fluorescence enhances visual contrast at depths below -10 m, where most light in the 'red' 600-700 nm range has been absorbed. Whereas the photoprotection hypothesis predicts fluorescence to be stronger near the surface and weaker in deeper water, the visual contrast hypothesis predicts the opposite. We used fluorometry to measure red fluorescence brightness in vivo in individuals belonging to eight common small reef fish species with conspicuously red fluorescent eyes. Fluorescence was significantly brighter in specimens from the -20 m sites than in those from -5 m sites in six out of eight species. No difference was found in the remaining two. Our results support the visual contrast hypothesis. We discuss the possible roles fluorescence may play in fish visual ecology and highlight the possibility that fluorescent light emission from the eyes in particular may be used to detect cryptic prey.

  17. Biliary fluorescent aromatic compounds (FACs) measured by fixed wavelength fluorescence (FF) in several marine fish species from the NW Mediterranean.

    Science.gov (United States)

    Insausti, David; Carrasson, Maite; Maynou, Francesc; Cartes, Joan E; Solé, Montserrat

    2009-11-01

    The fixed wavelength fluoresce (FF) method was used to estimate the levels of fluorescent aromatic compounds (FACs) in the bile of fourteen fish species of commercial and/or ecological interest. Sampling was carried out in the NW Mediterranean at depths ranging from 50 to 1000 m during four seasonal cruises. During the summer sampling period, some species were also collected from another site (Vilanova fishing grounds) for comparison. Baseline levels of the polycyclic aromatic hydrocarbons (PAHs) naphthalene, phenanthrene, pyrene and benzo[a]pyrene were measured. Some seasonality was observed, with reduced FF levels in summer and no differences among sites, consistent with sediment PAH levels. We discuss our results in relation to fish phylogeny, season, depth, diet, trophic level and swimming capacity. Overall FF levels indicated differences among species; the suprabenthic feeders from shallow and deep communities, and Mullus barbatus in particular, displayed elevated FF values and are potential candidates for additional monitoring studies.

  18. Interphase cytogenetics in pathology: principles, methods, and applications of fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Werner, M; Wilkens, L; Aubele, M; Nolte, M; Zitzelsberger, H; Komminoth, P

    1997-01-01

    Characteristic chromosome aberrations have been identified in various tumors. Fluorescence in situ hybridization (FISH) using specific probes that are generated by vector cloning or in vitro amplification and labeled with fluorescent dyes allow for the detection of these genetic changes in interphase cells. This technique, that is also referred to as "interphase cytogenetics", can be performed in cytological preparations as well as in sections of routinely formaldehyde-fixed and paraffin-embedded tissue. In cancer research and diagnostics, interphase cytogenetics by FISH is used to detect numerical chromosome changes and structural aberrations, e.g., translocations, deletions, or amplifications. In this technical overview, we explain the principles of the FISH method and provide protocols for FISH in cytological preparations and paraffin sections. Moreover, possible applications of FISH are discussed.

  19. Detection of polycyclic aromatic hydrocarbons (PAHs) in raw menhaden fish oil using fluorescence spectroscopy: Method development.

    Science.gov (United States)

    Pena, Edwin A; Ridley, Lauren M; Murphy, Wyatt R; Sowa, John R; Bentivegna, Carolyn S

    2015-09-01

    Raw menhaden fish oil was developed for biomonitoring polycyclic aromatic hydrocarbons (PAHs) using fluorescence spectroscopy. Menhaden (Genus Brevoortia) were collected in 2010 and/or 2011 from Delaware Bay, New Jersey, USA; James River, Virginia, USA; Vermillion Bay, Louisiana, USA (VBLA); and Barataria Bay, Louisiana, USA (BBLA). Barataria Bay, Louisiana received heavy oiling from the Deepwater Horizon oil spill. Method development included determining optimal wavelengths for PAH detection, fish oil matrix interferences, and influence of solvent concentration on extraction. Results showed that some fish oils contained high molecular weight PAH-like compounds in addition to other fluorescent compounds such as albumin and vitamin A and vitamin E. None of these naturally occurring compounds interfered with detection of high molecular weight PAHs. However, data suggested that the lipid component of fish oil was altering fluorescence spectra by supporting the formation of PAH excimers. For example, the most intense excitation wavelength for hydroxypyrene shifted from Ex285/Em430 to Ex340/Em430. Comparison of Deepwater Horizon crude oil and fish oil spectra indicated that some fish oils contained crude oil-like PAHs. Using wavelengths of Ex360/Em430, fish oil concentrations were calculated as 3.92 μg/g, 0.61 μg/g, and 0.14 μg/g for a Delaware Bay sample, BBLA 2011, and VBLA 2011, respectively. Overall, these results supported using menhaden fish oil to track PAH exposures spatially and temporally.

  20. Fluorescent in situ hybridization (FISH on the veterinary diagnostic field

    Directory of Open Access Journals (Sweden)

    Ján Dianovský

    2016-09-01

    Full Text Available Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+ was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative genomic hybridization numerous chromosomal rearrangements were detected, which indicated the heterogeneity in tumour growth. In Bernese Mountain Dog bitch,8 loses on chromosomes and gains on 18 different of chromosomes were detected. The last study was focused on chromosomal position and nucleotide sequencing of the LCA5L exons. Those were analysed in cattle of BTA1q44, sheep OAR1q43 and of CHI1q44 in the goats.

  1. DNA breakage detection-fluorescence in situ hybridization (DBD-FISH in buccal cells

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    E. I. Cortés-Gutiérrez

    2012-12-01

    Full Text Available DNA breakage detection-fluorescence in situ hybridization (DBD-FISH is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91. In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

  2. DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells.

    Science.gov (United States)

    Cortés-Gutiérrez, E I; Dávila-Rodríguez, M I; Fernández, J L; López-Fernández, C; Gosálvez, J

    2012-12-28

    DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a recently developed technique that allows cell-by-cell detection and quantification of DNA breakage in the whole genome or within specific DNA sequences. The present investigation was conducted to adapt the methodology of DBD-FISH to the visualization and evaluation of DNA damage in buccal epithelial cells. DBD-FISH revealed that DNA damage increased significantly according to H2O2 concentration (r2=0.91). In conclusion, the DBD-FISH technique is easy to apply in buccal cells and provides prompt results that are easy to interpret. Future studies are needed to investigate the potential applicability of a buccal cell DBD-FISH model to human biomonitoring and nutritional work.

  3. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

    Directory of Open Access Journals (Sweden)

    Niels Tommerup

    2010-11-01

    Full Text Available Fluorescence in situ Hybridization (FISH is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3–5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.

  4. Role of fluorescence in situ hybridization (FISH) in sequencing the tomato genome

    Science.gov (United States)

    Chromosomes at various stages of the cell cycle can be used for localization of DNA probes via Fluorescence in situ hybridization (FISH). While mitotic metaphase chromosomes are demonstrably too short and compact for this purpose, long pachytene chromosomes are ideal. BACs that hybridize to euchrom...

  5. Automation of ALK gene rearrangement testing with fluorescence in situ hybridization (FISH): a feasibility study.

    Science.gov (United States)

    Zwaenepoel, Karen; Merkle, Dennis; Cabillic, Florian; Berg, Erica; Belaud-Rotureau, Marc-Antoine; Grazioli, Vittorio; Herelle, Olga; Hummel, Michael; Le Calve, Michele; Lenze, Dido; Mende, Stefanie; Pauwels, Patrick; Quilichini, Benoit; Repetti, Elena

    2015-02-01

    In the past several years we have observed a significant increase in our understanding of molecular mechanisms that drive lung cancer. Specifically in the non-small cell lung cancer sub-types, ALK gene rearrangements represent a sub-group of tumors that are targetable by the tyrosine kinase inhibitor Crizotinib, resulting in significant reductions in tumor burden. Phase II and III clinical trials were performed using an ALK break-apart FISH probe kit, making FISH the gold standard for identifying ALK rearrangements in patients. FISH is often considered a labor and cost intensive molecular technique, and in this study we aimed to demonstrate feasibility for automation of ALK FISH testing, to improve laboratory workflow and ease of testing. This involved automation of the pre-treatment steps of the ALK assay using various protocols on the VP 2000 instrument, and facilitating automated scanning of the fluorescent FISH specimens for simplified enumeration on various backend scanning and analysis systems. The results indicated that ALK FISH can be automated. Significantly, both the Ikoniscope and BioView system of automated FISH scanning and analysis systems provided a robust analysis algorithm to define ALK rearrangements. In addition, the BioView system facilitated consultation of difficult cases via the internet.

  6. itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization.

    Science.gov (United States)

    Row, Richard H; Martin, Benjamin L

    2017-03-20

    Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining. By iterating the antibody staining and development steps, a strong signal can be obtained from probes that were previously too weak to detect.

  7. Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

    DEFF Research Database (Denmark)

    Ronander, Elena; Bengtsson, Dominique C; Joergensen, Louise;

    2012-01-01

    and the consequence of differential binding on the clinical outcome of P. falciparum infections. Recently, the mutually exclusive transcription paradigm has been called into doubt by transcription assays based on individual P. falciparum transcript identification in single infected erythrocytic cells using RNA...... fluorescent in situ hybridization (FISH) analysis of var gene transcription by the parasite in individual nuclei of P. falciparum IE(1). Here, we present a detailed protocol for carrying out the RNA-FISH methodology for analysis of var gene transcription in single-nuclei of P. falciparum infected human...... erythrocytes. The method is based on the use of digoxigenin- and biotin- labeled antisense RNA probes using the TSA Plus Fluorescence Palette System(2) (Perkin Elmer), microscopic analyses and freshly selected P. falciparum IE. The in situ hybridization method can be used to monitor transcription...

  8. Detection of integrated herpesvirus genomes by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Kaufer, Benedikt B

    2013-01-01

    Fluorescence in situ hybridization (FISH) is widely used to visualize nucleotide sequences in interphase cells or on metaphase chromosomes using specific probes that are complementary to the respective targets. Besides its broad application in cytogenetics and cancer research, FISH facilitates the localization of virus genomes in infected cells. Some herpesviruses, including human herpesvirus 6 (HHV-6) and Marek's disease virus (MDV), have been shown to integrate their genetic material into host chromosomes, which allows transmission of HHV-6 via the germ line and is required for efficient MDV-induced tumor formation. We describe here the detection by FISH of integrated herpesvirus genomes in metaphase chromosomes and interphase nuclei of herpesvirus-infected cells.

  9. Breeding and reproduction of transgenic fish fluorescent in captivity: a prospective scope

    Directory of Open Access Journals (Sweden)

    Carlos Scotto Espinoza

    2012-03-01

    Full Text Available The introduction, breeding and hybridization of fluorescent transgenic fish in Peruvian territory has been going since 2006 as demonstrated in laboratory tests reported in this article. He has spoken to Peru Law No. 29811 establishing the moratorium on the production of income and living modified organisms into the country for a period of 10 years for cultivation or breeding, including water, to be released into the environment. The paper shows the existence of aquatic LMOs that are being propagated commercially by aquarists and / or ornamental fish farmers out of control and could potentially be released into the environment. However, preliminary results on the other side could initiate a first analysis and risk management at the laboratory and / or under controlled conditions with these organisms (zebra fish case that could provide the basis for future decision making relevant to the introduction of GMOs for any scientific purpose, commercial, environmental or other national interest not yet glimpsed.

  10. Risk stratification of plasma cell neoplasm: insights from plasma cell-specific cytoplasmic immunoglobulin fluorescence in situ hybridization (cIg FISH) vs. conventional FISH.

    Science.gov (United States)

    Dong, Henry; Yang, Hai-Su; Jagannath, Sundar; Stephenson, Christine F; Brenholz, Pauline; Mazumder, Amitabha; Chari, Ajai

    2012-10-01

    We directly compared the results of routine fluorescence in situ hybridization (FISH) and plasma cell-specific cytoplasmic immunoglobulin (cIg) FISH from 75 paired samples for myeloma risk stratification. CIg FISH improves test specificity and sensitivity and tends to eliminate borderline results. It proves that most plasma cells (PCs) consistently carry the abnormality in myelomas with an IGH translocation, whereas routine FISH detects these cells only at variably low levels. Routine cytogenetic analysis of plasma cell neoplasms (PCNs) has a low sensitivity. Conventional fluorescence in situ hybridization (FISH) is not plasma cell (PC) specific and results are diluted by other cells in the sample. Although PC-specific FISH testing has been recommended for multiple myeloma (MM) risk stratification, eg, by combining cytoplasmic immunoglobulin (cIg) staining with FISH, the benefits of cIg FISH have never been directly demonstrated in a controlled study. Seventy-five samples from patients with PCNs were analyzed by concomitant conventional FISH and cIg FISH with probes for t(4;14), t(11;14), t(14;16), -13, 17p-, and +3. The results were compared for their reliability, specificity, and consistency. Apart from marginally improving detection threshold in samples with low PC burden, cIg FISH identified more abnormal cases (50 vs. 47 cases) and more chromosome abnormalities (113 vs. 103 events) than did conventional FISH. It differentiated del(13q) in myelodysplasia from MM. Remarkably, cIg FISH consistently identified a high percentage of abnormal PCs in all cases. It detected IGH translocation in 78% to 100% of PCs in all but 2 positive cases, whereas conventional FISH detected 0% to 46% in these cases (median, 91% vs. 9%). The abnormal cells found in patients with 17p- were 19% to 96% by cIg FISH vs. 0% to 13% by conventional FISH (median, 54% vs. 9%). Cases with insufficient PCs for cIg FISH had only normal conventional FISH results. CIg FISH improves reliability of

  11. Detection of a complex translocation using fluorescent in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Rosen, B.A. [Brandeis Univ., Waltham, MA (United States); Abuelo, D.N. [Rhode Island Hospital, Providence, RI (United States); Mark, H.F. [Brown Univ. School of Medicine, Providence, RI (United States)

    1994-09-01

    The use of fluorescent in situ hybridization (FISH) allowed the detection of a complex 3-way translocation in a patient with multiple congenital malformations and mental retardation. The patient was a 10-year-old girl with mental retardation, seizures, repaired cleft palate, esotropia, epicanthal folds, broad nasal bridge, upward slanting palpebral fissures, single transverse palmar crease, brachydactyly, hypoplastic nails, ectrodactyly between the third and fourth right toes, and hypoplasia of the left third toe. Chromosome analysis performed at birth was reported as normal. We performed high resolution banding analysis which revealed an apparently balanced translocation between chromosomes 2 and 9. However, because of her multiple abnormalities, further studies were ordered. Fluorescent in situ hybridization (FISH) using chromosome painting probes revealed a karyotype of 46,XX,t(2;8;9) (2pter{yields}q31::8q21.2{yields}8qter; 8pter{yields}q21.2::2q31{yields}q34::9q34{yields}qter; 9pter{yields}q34::2q34{yields}qter). The 3-way translocation appears to be de novo, as neither parent is a translocation carrier. This case illustrates the importance of using FISH to further investigate cases of apparently balanced translocations in the presence of phenotypic abnormalities and/or mental retardation.

  12. Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Angelidis, Apostolos S; Tirodimos, Ilias; Bobos, Mattheos; Kalamaki, Mary S; Papageorgiou, Demetrios K; Arvanitidou, Malamatenia

    2011-12-02

    The transmission pathways of Helicobacter pylori in humans have not been fully elucidated. Research in the last decade has proposed that foodborne transmission, among others, may be a plausible route of human infection. Owing to the organism's fastidious growth characteristics and its ability to convert to viable, yet unculturable states upon exposure to stress conditions, the detection of H. pylori in foods via culture-dependent methods has been proven to be laborious, difficult and in most cases unsuccessful. Hence, nucleic acid-based methods have been proposed as alternative methods but, to date, only PCR-based methods have been reported in the literature. In the current study, fluorescence in situ hybridization (FISH) was used for the detection of H. pylori in raw, bulk-tank bovine milk. After repeated milk centrifugation and washing steps, the bacterial flora of raw milk was subjected to fixation and permeabilization and H. pylori detection was conducted by FISH after hybridization with an H. pylori-specific 16S rRNA-directed fluorescent oligonucleotide probe. Using this protocol, H. pylori was detected in four out of the twenty (20%) raw milk samples examined. The data presented in this manuscript indicate that FISH can serve as an alternative molecular method for screening raw bovine milk for the presence of H. pylori.

  13. Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH).

    Science.gov (United States)

    Vilas Boas, Diana; Almeida, Carina; Sillankorva, Sanna; Nicolau, Ana; Azeredo, Joana; Azevedo, Nuno F

    2016-01-01

    Bacteriophage-host interaction studies in biofilm structures are still challenging due to the technical limitations of traditional methods. The aim of this study was to provide a direct fluorescence in situ hybridization (FISH) method based on locked nucleic acid (LNA) probes, which targets the phage replication phase, allowing the study of population dynamics during infection. Bacteriophages specific for two biofilm-forming bacteria, Pseudomonas aeruginosa and Acinetobacter, were selected. Four LNA probes were designed and optimized for phage-specific detection and for bacterial counterstaining. To validate the method, LNA-FISH counts were compared with the traditional plaque forming unit (PFU) technique. To visualize the progression of phage infection within a biofilm, colony-biofilms were formed and infected with bacteriophages. A good correlation (r = 0.707) was observed between LNA-FISH and PFU techniques. In biofilm structures, LNA-FISH provided a good discrimination of the infected cells and also allowed the assessment of the spatial distribution of infected and non-infected populations.

  14. Prediction of melting temperatures in fluorescence in situ hybridization (FISH) procedures using thermodynamic models.

    Science.gov (United States)

    Fontenete, Sílvia; Guimarães, Nuno; Wengel, Jesper; Azevedo, Nuno Filipe

    2016-01-01

    The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermodynamics that provide reasonably accurate thermodynamic information on nucleic acid duplexes and allow estimation of the melting temperature. Because there are no thermodynamic models specifically developed to predict the hybridization temperature of a probe used in a fluorescence in situ hybridization (FISH) procedure, the melting temperature is used as a reference, together with corrections for certain compounds that are used during FISH. However, the quantitative relation between melting and experimental FISH temperatures is poorly described. In this review, various models used to predict the melting temperature for rRNA targets, for DNA oligonucleotides and for nucleic acid mimics (chemically modified oligonucleotides), will be addressed in detail, together with a critical assessment of how this information should be used in FISH.

  15. One for all--a highly efficient and versatile method for fluorescent immunostaining in fish embryos.

    Directory of Open Access Journals (Sweden)

    Daigo Inoue

    Full Text Available BACKGROUND: For the detection and sub-cellular (co-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval protocol so that optimizing immunostaining turned out to be tedious and time consuming. METHODOLOGY/PRINCIPAL FINDING: Here we present a novel method to efficiently retrieve the antigen in a widely applicable standard protocol, facilitating fluorescent immunostaining of both cryosections and whole mount preparations in zebrafish (Danio rerio and medaka (Oryzias latipes. CONCLUSIONS/SIGNIFICANCE: Our method overcomes the loss of sections and damage of tissue and cell morphology, and allows parallel immunostaining in multiple colors, co-immunostaining with fluorescent proteins in transgenic fish lines and in combination with whole mount in situ hybridization.

  16. Screening for dioxin contamination in fish oil by PARAFAC and N-PLSR analysis of fluorescence landscapes

    DEFF Research Database (Denmark)

    Pedersen, Dorthe Kjær; Engelsen, Søren Balling

    2002-01-01

    A preliminary investigation of fish oils demonstrates that fluorescence excitation-emission landscapes evaluated by 3-way chemometric methods may be a candidate for an inexpensive screening method to indicate the level of contamination by dioxins and PCB’s which are normally analysed with expensive...... and time-consuming physicochemical separation techniques such as GC-MS. Fluorescence landscapes of 88 fish oils have been investigated and showed great variation due to species, season and treatment, depicting a variation in natural fluorescent components. The fluorescence landscapes were analysed...... by PARAFAC. Samples with similar fluorescence fingerprints were selected from a PARAFAC score plot and local significant prediction models with PARAFAC/MLR, N-PLSR and PLSR were established with correlation coefficients in the range from r=0.69 (n=10) to r=0.97 (n=75) for dioxin and r=0.92 (n=12) for PCB...

  17. Fluorescence in situ hybridization (FISH analysis of primary ocular adnexal MALT lymphoma

    Directory of Open Access Journals (Sweden)

    Harada Mine

    2006-10-01

    Full Text Available Abstract Background It remains unknown whether primary ocular adnexal extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma is a homogeneous entity, as there are few reports of the results of cytogenetic or molecular analyses of these tumors. Methods We performed interphase fluorescence in situ hybridization (FISH analysis to detect translocations and aneuploidy in 34 cases of primary ocular adnexal MALT lymphoma, and reviewed the histopathological findings. Correlations between the results of FISH analysis, the histopathological features and the clinical data were also analyzed. Results Among the 34 cases, FISH analysis revealed t(14;18(q32;q21 in one case, trisomy 3 in 21 cases (62%, and trisomy 18 in 16 cases (47%. The cases with trisomy 18 had significantly more prominent lymphoepithelial lesions (LELs and less nodularity in the tumors. In regard to the clinical correlations, tumors with trisomy 18 were observed predominantly in females and younger patients; also, in the majority of the cases, the tumor was of conjunctival origin. All the cases with recurrence showed trisomy 18 in the tumor. Conclusion Primary ocular adnexal MALT lymphoma is a significantly heterogeneous entity. Cases with trisomy 18 may have unique clinicopathological features.

  18. Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Deloge-Abarkan, Magali; Ha, Thi-Lan; Robine, Enric; Zmirou-Navier, Denis; Mathieu, Laurence

    2007-01-01

    Aerosols of water contaminated with Legionella bacteria constitute the only mode of exposure for humans. However, the prevention strategy against this pathogenic bacteria risk is managed through the survey of water contamination. No relationship linked the Legionella bacteria water concentration and their airborne abundance. Therefore, new approaches in the field of the metrological aspects of Legionella bioaerosols are required. This study was aimed at testing the main principles for bioaerosol collection (solid impaction, liquid impingement and filtration) and the in situ hybridization (FISH) method, both in laboratory and field assays, with the intention of applying such methodologies for airborne Legionella bacteria detection while showering. An aerosolization chamber was developed to generate controlled and reproducible L. pneumophila aerosols. This tool allowed the identification of the liquid impingement method as the most appropriate one for collecting airborne Legionella bacteria. The culturable fraction of airborne L. pneumophila recovered with the liquid impingement principle was 4 and 700 times higher compared to the impaction and filtration techniques, respectively. Moreover, the concentrations of airborne L. pneumophila in the impinger fluid were on average 7.0 x 10(5) FISH-cells m(-3) air with the fluorescent in situ hybridization (FISH) method versus 9.0 x 10(4) CFU m(-3) air with the culture method. These results, recorded under well-controlled conditions, were confirmed during the field experiments performed on aerosols generated by hot water showers in health institutions. This new approach may provide a more accurate characterization of aerobiocontamination by Legionella bacteria.

  19. [Clinical use of the ImmunoCyt/uCyt+ and fluorescence in situ hybridisation (FISH) tests for urothelial carcinomas].

    Science.gov (United States)

    Lodde, Michele; Mian, Christine

    2013-01-01

    In recent decades years, we have witnessed the propagation and marketing of numerous diagnostic tests capable of detecting, in the urine of patients, the presence of urothelial tumor markers. Among None of the different markers studied to date , no one has been able to meet all the requirements of the ideal marker. We present and discuss below we discuss the results reported in the literature of about two tests approved by the Food and Drug Administration [ImmunoCyt/uCyt+ and Fluorescence In Situ Hybridisation (FISH)], which have been and commercially available for about 10 years., ImmunoCyt/uCyt + and Fluorescence In Situ Hybridisation (FISH).

  20. The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa.

    Science.gov (United States)

    Flajshans, Martin; Cosson, Jacky; Rodina, Marek; Linhart, Otomar

    2004-01-01

    The viability of spermatozoa has been assessed using SYBR 14 staining for DNA of living cells and propidium iodide staining for DNA of degenerate cells. This dual staining was performed on four fish species (Siberian sturgeon, Acipenser baerii; common carp, Cyprinus carpio; tench, Tinca tinca and wels, Silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. Ten phase contrast and epifluorescent images were recorded per sample, corresponding images were overlaid, and the blended images were evaluated for live and dead spermatozoa, represented by green and red fluorescence signals. Live/dead proportions were assessed, after dual thresholding, by imaging software that counted absolute numbers of objects and computed their frequencies. All sperm heads were found to be labelled, emitting either green or red light. Mean numbers of spermatozoa per image were in the ranges 32-113, 61-105, 48-104 and 29-91 for Siberian sturgeon, common carp, tench and wels, respectively. The corresponding proportions of live spermatozoa were in the ranges 83.56-94.59%, 93.92-97.02%, 76.14-97.76% and 79.45-83.76%. Standard deviations did not exceed 5% of the means. The image cytometric system using dual staining with SYBR 14 and propidium iodide was clearly suitable for assessing the viability of freshwater fish spermatozoa.

  1. Fluorescent in situ hybridization (FISH) assessment of chromosome copy number in sperm

    Energy Technology Data Exchange (ETDEWEB)

    Sheu, M.; Sigman, M.; Mark, H.F.L. [Brown Univ. School of Medicine, Providence, RI (United States)

    1994-09-01

    Approximately 15% of all recognized pregnancies end in spontaneous abortions. The overall frequency of chromosome abnormalities in spontaneous abortions is approximately 50%. Thus aneuploidy is a significant cause of fetal wastage. In addition, structural and numerical abnormalities of chromosomes can also lead to birth defects, developmental delay, mental retardation and infertility. Conventional cytogenetic analysis via GTG- and other banding techniques is a powerful tool in the elucidation of the nature of chromosomal abnormalities. Fluorescent in situ hybridization (FISH) enables detection of numerical chromosomal abnormalities, especially trisomies, in intact cells. Using FISH and commercially available biotin-labeled probes, we have initiated a prospective study to assess specific chromosome copy number of preparations of unstained smears from men referred for a male infertility evaluation as well as smears from normal control males chosen randomly from the sample of sperm donors. A total of approximately 19,000 sperm nuclei have been examined thus far. Of those suitable for analysis, 7382 (38.75%) were normal possessing one copy of chromosome 8, 155 (0.81%) were disomic, and 15 (0.079%) had more than two copies of chromosome 8. Comparisons with data available in the literature will be discussed. Work is ongoing to increase the efficiency of hybridization using both reported and previously untried pretreatment and fixation protocols. We have also initiated studies using multicolor FISH with various chromosome enumeration probes. The assay described here is a potentially powerful tool for detecting rare events such as spontaneous germ cell aneuploidy, aneuploidy detected in semen from men with carcinoma in situ of the testis and aneuploidy induced by potential environmental genotoxicants. It can also be utilized for segregation analysis and for correlating chromosome copy number with germ cell morphology.

  2. PENINDAKAN ILLEGAL FISHING DAN PERJANJIAN BILATERAL BIDANG PERIKANAN DENGAN NEGARA TETANGGA

    Directory of Open Access Journals (Sweden)

    Ms. Koesrianti

    2008-06-01

    Full Text Available Indonesia is the biggest maritim state in the world as two third of its area are ocean. In order to maximized its fisheries products, Indonesia has some policies regarding to the management of its waters, including eradicating illegal logging and reforming bilateral agreements with some neighbor countries. This policies, however, appear to be not yet optimal. The government in 1985 has ratified the UN Convention on the Law of the Sea (UNCLOS 1982 with the Act 17/1985. However, up to now, Indonesia has not ratified yet the United Nations Fish Stocks Agreement 1995 and the 1993 Compliance Agreement as the continued arrangements of UNCLOS 1982. Indonesia should conduct such ratification in order to optimize the Indonesian fisheries industry in which its natural resources, whether living or non-living can be explored and exploited. This is crucial as fisheries industries can provide fish stock as alternative healthy food for the people in Indonesia.

  3. Multiplex fluorescence in situ hybridization (M-FISH) and confocal laser scanning microscopy (CLSM) to analyze multispecies oral biofilms.

    Science.gov (United States)

    Karygianni, Lamprini; Hellwig, Elmar; Al-Ahmad, Ali

    2014-01-01

    Multiplex fluorescence in situ hybridization (M-FISH) constitutes a favorable microbiological method for the analysis of spatial distribution of highly variable phenotypes found in multispecies oral biofilms. The combined use of confocal laser scanning microscopy (CLSM) produces high-resolution three-dimensional (3D) images of individual bacteria in their natural environment. Here, we describe the application of M-FISH on early (Streptococcus spp., Actinomyces naeslundii) and late colonizers (Fusobacterium nucleatum, Veillonella spp.) of in situ-formed oral biofilms, the acquisition of CLSM images, as well as the qualitative and quantitative analysis of these digitally obtained and processed images.

  4. Development of a fluorescent in situ hybridization (FISH) technique for visualizing CGMMV in plant tissues.

    Science.gov (United States)

    Shargil, D; Zemach, H; Belausov, E; Lachman, O; Kamenetsky, R; Dombrovsky, A

    2015-10-01

    Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors.

  5. A reusable and sensitive biosensor for total mercury in canned fish based on fluorescence polarization.

    Science.gov (United States)

    Shen, Tongfei; Yue, Qiaoli; Jiang, Xiuxiu; Wang, Lei; Xu, Shuling; Li, Haibo; Gu, Xiaohong; Zhang, Shuqiu; Liu, Jifeng

    2013-12-15

    In this work, we developed a sensitive and selective sensor technique for total mercury (Hg) detection in canned fish samples based on the fluorescence polarization (FP) method. The detection principle was that ssDNA containing thymine (T) bases was modified on magnetic nanoparticles (MNPs), which were used as enhancement probe. In the presence of Hg(2+), the ssDNA on MNPs can hybridize with the fluorophore labeled aptamer owing to the specific interaction between T bases and Hg(2+). The formation of thymine-Hg(2+)-thymine (T-Hg(2+)-T) complexes leads to the molar mass increase of fluorophore molecules, resulting in the enhancement of FP signal. The increase of FP was in a good linearity with the concentration of Hg(2+) in range of 2.0 nM-1.0 mM and the limit of detection was 0.49 nM (3.29 SB/m, according to the recent recommendation of IUPAC). Moreover, the proposed biosensor can be reused for 6 cycling times and was successfully applied in monitoring Hg(2+) in real samples.

  6. Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization

    OpenAIRE

    Germán Robledo; Guillermo Seijo

    2008-01-01

    Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with a...

  7. Studies of the Ecophysiology of Single Cells in Microbial Communities by (Quantitative) Microautoradiography and Fluorescence In Situ Hybridization (MAR-FISH)

    DEFF Research Database (Denmark)

    Nierychlo, Marta; Nielsen, Jeppe Lund; Nielsen, Per Halkjær

    2015-01-01

    Microautoradiography (MAR) in combination with fluorescence in situ hybridization (FISH) is a powerful method of obtaining information about the ecophysiology of probe-defined single cells in mixed microbial communities. The incorporation of radiolabelled substrates can be quantified by automated...

  8. LED-FISH: Fluorescence microscopy based on light emitting diodes for the molecular analysis of Her-2/neu oncogene amplification

    Directory of Open Access Journals (Sweden)

    Vollmer Ekkehard

    2008-12-01

    Full Text Available Abstract Light emitting diodes (LED, which are available as small monochromatic light sources with characteristic features such as maximum illumination power combined with minimum energy consumption and extremely long lifespan have already proved as a highly potential low-cost alternative for specific diagnostic applications in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the most reliable evaluation of Her-2/neu (c-erbB2 gene amplification, which has been established in the last few years for routine diagnosis in clinical pathology as determinant towards Herceptin-based treatment of patients with breast cancer, is based on fluorescence in situ hybridization (FISH and corresponding high priced fluorescence equipment. In order to test the possibility to utilize the advantages of low-cost LED technology on FISH analysis of c-erbB2 gene expression for routine diagnostic purposes, the applicability of a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER* LED Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination with a conventional 100W mercury vapor lamp. Both microscopes were fitted with the same Quicam FAST CCD digital camera to unequivocally compare the quality of the captured images. C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. The Her2/neu gene signals (green were identifiable in the tumor cells in all cases and images of equal quality were captured under almost identical conditions by 480 nm (blue LED module equipped standard Axiostar microscope as compared to conventional fluorescence microscopy. In this first attempt, these monochromatic LED elements proved in principle to be suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own

  9. 21 CFR 866.4700 - Automated fluorescence in situ hybridization (FISH) enumeration systems.

    Science.gov (United States)

    2010-04-01

    ... detection and enumeration of FISH signals in interphase nuclei of formalin-fixed, paraffin-embedded human.... This device is intended for in vitro diagnostic use with FISH assays as an aid in the...

  10. Fluorescent in-situ hybridization (FISH for BCR/ABL in chronic myeloid leukemia after bone marrow transplantation

    Directory of Open Access Journals (Sweden)

    Maria de Lourdes Lopes Ferrari Chauffaille

    2001-01-01

    Full Text Available CONTEXT: Identification of Philadelphia chromosome or BCR/ABL gene rearrangement in chronic myeloid leukemia is important at diagnosis as well as after treatment. OBJECTIVE: To compare the results of karyotyping using fluorescent in-situ hybridization (FISH upon diagnosis and 1 year after bone marrow transplantation in 12 patients. TYPE OF STUDY: Diagnostic test and residual disease detection. SETTING: Hematology and Hemotherapy Department, Federal University of São Paulo/Escola Paulista de Medicina, São Paulo, Brazil. SAMPLE: 12 patients with chronic myeloid leukemia at diagnosis and 1 year after bone marrow transplantation. DIAGNOSTIC TEST: Karyotyping was done in the usual way and the BCR/ABL gene-specific probe was used for FISH. MAIN MEASUREMENTS: Disease at diagnosis and residual. RESULTS: At diagnosis, 10 patients presented t(9;22(q34.1;q11 as well as positive FISH. Two cases did not have metaphases but FISH was positive. After bone marrow transplantation, 8 patients presented normal karyotype, 1 had persistence of identifiable Philadelphia chromosome and 3 had no metaphases. Two cases showed complete chimera and 2 had donor and host cells simultaneously. FISH was possible in all cases after bone marrow transplantation and confirmed the persistence of identifiable Philadelphia chromosome clone in one patient, and identified another that did not present metaphases for analysis. Cases that showed mixed chimera in karyotype were negative for BCR/ABL by FISH. CONCLUSION: The applicability of FISH is clear, particularly for residual disease detection. Classical and molecular cytogenetics are complementary methods.

  11. Fully automated fluorescent in situ hybridization (FISH staining and digital analysis of HER2 in breast cancer: a validation study.

    Directory of Open Access Journals (Sweden)

    Elise M J van der Logt

    Full Text Available HER2 assessment is routinely used to select patients with invasive breast cancer that might benefit from HER2-targeted therapy. The aim of this study was to validate a fully automated in situ hybridization (ISH procedure that combines the automated Leica HER2 fluorescent ISH system for Bond with supervised automated analysis with the Visia imaging D-Sight digital imaging platform. HER2 assessment was performed on 328 formalin-fixed/paraffin-embedded invasive breast cancer tumors on tissue microarrays (TMA and 100 (50 selected IHC 2+ and 50 random IHC scores full-sized slides of resections/biopsies obtained for diagnostic purposes previously. For digital analysis slides were pre-screened at 20x and 100x magnification for all fluorescent signals and supervised-automated scoring was performed on at least two pictures (in total at least 20 nuclei were counted with the D-Sight HER2 FISH analysis module by two observers independently. Results were compared to data obtained previously with the manual Abbott FISH test. The overall agreement with Abbott FISH data among TMA samples and 50 selected IHC 2+ cases was 98.8% (κ = 0.94 and 93.8% (κ = 0.88, respectively. The results of 50 additionally tested unselected IHC cases were concordant with previously obtained IHC and/or FISH data. The combination of the Leica FISH system with the D-Sight digital imaging platform is a feasible method for HER2 assessment in routine clinical practice for patients with invasive breast cancer.

  12. Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe

    Institute of Scientific and Technical Information of China (English)

    LV Xue-fei; ZHAO Yi-bing; ZHOU Qun-fang; JIANG Gui-bin; SONG Mao-yong

    2007-01-01

    A sensitive method based on the fluorescence quenching effect of the Tb3+-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.

  13. Fish and Female Agency in a Madurese Fishing Village in Indonesia Poissardes et poissons à Madura. Femmes en action dans un village de pêcheurs d’Indonésie

    Directory of Open Access Journals (Sweden)

    Anke Niehof

    2012-12-01

    Full Text Available Le rôle crucial des femmes dans l’économie halieutique locale et leur haut degré d’autonomie sociale constituaient deux traits remarquables de l’organisation socio-économique du village de pêcheurs de Patondu (Madura en 1978. Un nouveau travail de terrain en 2004 montre que se sont produits au cours de ces 26 ans dans la pêcherie des changements technologiques et économiques de grande portée. De plus, avec la mise en place d’équipements publics, Patondu est désormais mieux relié au monde extérieur. En 2004, pourtant, les femmes y ont, autant sinon plus qu’avant, une place éminente. Les cas de six femmes actives dans le commerce et le traitement du poisson et le financement de la pêche sont examinés. Les pangamba’, considérées dans la littérature comme formant « un groupe phénoménal » , méritent une attention particulière : ces femmes – marchandes, entrepreneures et banquières, tout à la fois – sont les pivots de l’économie halieutique, comme « matrones » dans les relations de « matronage » qui structurent les réseaux socio-économiques. La solide position des femmes repose sur une division sexuelle du travail, écologiquement et culturellement fondée dans la société de Patondu, qui leur ouvre un vaste champ d’opportunités d’action dans les sphères économique et sociale. La vie quotidienne de Patondu s’apprécie en ichtyo-valeur et les femmes déterminent cette valeur. Elles contrôlent donc, dans une large mesure, l’économie halieutique locale. De plus, la limite fluide entre les sphères domestique et économique évite aux femmes le confinement au foyer et leur permet de jouer ce rôle de matrones dans la sphère économique. Des recherches sur les commerçantes des marchés à Java ont produit des résultats similaires. La société de Patondu n’est pas égalitaire et, même si le genre, comme facteur de stratification, recoupe d’autres facteurs et si le hiatus

  14. Clinical Application of Fluorescence in Situ Hybridization (FISH) to Detect HER-2 Gene in Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    Maurie Buehler; Ellie Guardino; Jung Sik Park; Eun Jeong Jang

    2014-01-01

    Objective: To investigate the clinical application of the detection of HER-2 gene by lfuorescence in situ hybridization (FISH) in breast cancer and the correlation between HER-2 gene ampliifcation and clinicopathology of breast cancer. Methods:Parafifn-embedded breast inifltrating ductal carcinoma from 48 patients were detected by FISH and immunohistochemistry (IHC) respectively for comparing the results of two methods. Results: HER-2 protein expressions were classified into three groups (3+/2+/1+ or 0) and the positive rates of HER-2 gene ampliifcation by FISH were 77.8%, 57.1% and 10.5%, respectively. Of the 29 cases with positive axillary lymph node, 12 were with HER-2 gene ampliifcation (P0.05). Conclusion:The false positive and negative rates are higher in HER-2 protein expression by IHC. Compared with IHC, FISH, being more effective and precise, can be applied extensively in clinic. HER-2 gene ampliifcation is concerned with axillary nodes metastases.

  15. KANDUNGAN VITAMIN B6, B9, B12 DAN E BEBERAPA JENIS DAGING, TELUR, IKAN DAN UDANG LAUT DI BOGOR DAN SEKITARNYA (VITAMIN B6, B9, B12 AND E CONTENT OF SEVERAL TYPES OF MEATS, EGGS, FISHES AND MARINE SHRIMPS IN BOGOR AND SURROUNDING AREAS

    Directory of Open Access Journals (Sweden)

    Heru Yuniati

    2012-06-01

    Full Text Available ABSTRACT Food Composition Table (DKBM in Indonesia has not mentioned all types of nutrients available in the food, particularly vitamin B6, B9 (folic acid, B12, and vitamin E. Therefore this study aimed to analyze the content of vitamin B6, B9 (folic acid, B12, and vitamin E in several types of meat, eggs, fish and marine shrimps consumed in Bogor and surrounding areas. Vitamin B6, B9, B12, and vitamin E from three kinds of meat (chicken, beef, lamb, two types of eggs (chicken, duck, and four species of fish (snapper, bloating, carp and tuna and crayfish are analyzed using High-Performance Liquid Chromatography (HPLC. The samples used are raw and taken from three locations in Bogor and surrounding areas. Fishes, meats and eggs contain high levels of folic acid, however the amount of folic acid content in meat varies depending on which part of meat the samples are taken, types of organ, and the fat content of the meat. The folic acid content in chicken wings is different with those in thigh. In fatty mutton the folic acid is higher than in those lean meat, and in yolk is higher than those in egg white. Vitamin E content of snapper is the highest amongs other types of fishes (6.54 µg/100 g.Chicken eggs contain a higher amount of vitamin E than duck eggs, while the yolk contains ahigher amount of vitamin E than those egg white. Keywords: animal foods, vitamin B6, vitamin B9 (folic Acid, vitamin B12, vitamin E   ABSTRAK Daftar Komposisi Bahan Makanan (DKBM yang ada di Indonesia belum memuat semua jenis zat gizi dalam makanan, khususnya vitamin B6, B9 (asam folat, B12 dan vitamin E. Menganalisis kandungan vitamin B6, B9 (asam folat, B12, dan vitamin E dalam beberapa jenis daging, telur, ikan dan udang laut yang dikonsumsi masyarakat di Bogor dan sekitarnya. Kandungan vitamin B6, B9, B12 dan vitamin E dari tiga jenis daging (ayam, sapi, kambing, dua jenis telur (ayam, itik, serta empat jenis ikan (kakap, kembung, mas, tongkol dan udang laut

  16. Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

    OpenAIRE

    Ansari, Helal A.; Ellison, Nicholas W; Shalome A. Bassett; Hussain, Syed W.; Bryan, Gregory T.; Williams, Warren M

    2016-01-01

    Background The unambiguous identification of individual chromosomes is a key part of the genomic characterization of any species. In this respect, the development and application of chromosome banding techniques has revolutionised mammalian and especially, human genomics. However, partly because of the traditional use of chromosome squash preparations, consistent fluorescence banding has rarely been achieved in plants. Here, successful fluorescence chromosome banding has been achieved for the...

  17. Analysis of fish bile with HPLC — fluorescence to determine environmental exposure to benzo(a)pyrene

    Science.gov (United States)

    Johnston, Eric P.; Baumann, Paul C.

    1989-01-01

    Brown bullhead from the Black River, Ohio, have a high incidence of liver neoplasia which is associated with elevated concentrations of polynuclear aromatic hydrocarbons (PAHs) in the sediment. We evaluated the use of biliary concentrations of benzo(a)pyrene [B(a)P] equivalents as a means for determining PAH exposure. Bile was collected from 16 brown bullheads and 8 common carp taken from each of two Lake Erie tributaries in Ohio, the industrialized Black River and the non-industrialized Old Woman Creek. Hatchery bullhead (n = 8) were used to determine base levels of PAHs. A high performance liquid chromatography (HPLC) — fluorescence technique was used to determine the concentration of B(a)P equivalents in the bile samples. The area of all peaks fluorescing at 380/430 nm was summed to give a single value for B(a)P equivalents in each sample. Concentrations of B(a)P equivalents generally reflected concentrations of PAH in sediment where fish were collected. Bile taken from Black River carp contained the highest concentration of B(a)P equivalents and was significantly different from all other groups. The value obtained for Black River bullhead was also high and was found to be significantly different from hatchery bullhead. B(a)P equivalents varied between carp and bullhead from the same habitat possibly because of differing food habits or metabolic pathways. However, our results indicate that relative levels of B(a)P equivalents in the bile of fish correspond well to B(a)P levels in sediment and may offer a means of determining environmental exposure of fish to the parent compound.

  18. [Determining the level of histamine in canned fish by the fluorometric method using the Spekol-10 apparatus with FK device for fluorescence spectrometry].

    Science.gov (United States)

    Fonberg-Broczek, M; Sawilska-Rautenstrauch, D; Windyga, B

    1991-01-01

    The content of histamine in canned fish products was determined by fluorometric method recommended by AOAC with final determination of fluorescence on spectrophotometer Spekol 10 with FKC equipment of wave excitation for maximum of emission: lambda = 365 nm, filter GVK--48). The method makes possible quick, reproducible, and sensitive determination (limit of detection: 1 mg/100 g of product) of histamine in canned fish products, on simple equipment available in every laboratory of count Sanitary Stations.

  19. Assessment of chromosomal abnormalities in sperm of infertile men using sperm karyotyping and multicolour fluorescence in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Moosani, N.; Martin, R.H. [Alberta Children`s Hospital and Univ. of Calgary (Canada)

    1994-09-01

    Individuals with male factor infertility resulting from idiopathic oligo-, astheno- or teratozoospermia are frequently offered IVF in an attempt to increase their chances of having a child. A concern remains whether these infertile males have an elevated risk of transmitting chromosomal abnormalities to their offspring. Sperm chromosomal complements from these men were assayed using the human sperm/hamster oocyte fusion system and fluorescence in situ hybridization (FISH) on sperm nuclei. For each of 5 infertile patients, 100 sperm karyotypes were analyzed and multicolour FISH analysis was performed on a minimum of 10,000 sperm nuclei for each chromosome-specific DNA probe for chromosomes 1 (pUC1.77), 12 (D12Z3), X (XC) and Y (DYZ3). As a group, the infertile patients showed increased frequencies of both numerical ({chi}{sup 2}=17.26, {proportional_to} <0.001) and total abnormalities ({chi}{sup 2}=7.78, {proportional_to} <0.01) relative to control donors when assessed by sperm karyotypes. Analysis of sperm nuclei by FISH indicated a significant increase in the frequency of disomy for chromosome 1 in three of the five patients as compared to control donors ({chi}{sup 2}>8.35, {proportional_to} <0.005). In addition, the frequency of XY disomy was significantly higher in four of the five patients studied by FISH ({chi}{sup 2}>10.58, {proportional_to}<0.005), suggesting that mis-segregation caused by the failure of the XY bivalent to pair may play a role in idiopathic male infertility.

  20. Efektivitas Minyak Ikan Lemuru Terproteksi Terhadap Populasi Mikrob Rumen dan Fermentasinya pada Kerbau dan Sapi (THE EFFECT OF PROTECTED LEMURU FISH OIL ON RUMEN MICROBES AND ITS FERMENTATION IN BUFFALOES AND CATTLE

    Directory of Open Access Journals (Sweden)

    Yurleni .

    2013-11-01

    Full Text Available The objective of this experiment was to examine the effect of protected lemuru fish oil on rumenmicrobes and its fermentation  in buffalo and cattle fattened in feedlot.  Six  male buffaloes and eight malecattle aged between 1,5-2 years old with initial live weight of 218,66±16,28 kg and 217,37±15,44 kg,respectively, were used in this study. They were fattened in feedlot using 35% forage and 65% concentratediet for 2,5 months. The protected lemuru fish oil was in the form of dried carboxylate salt mixture (DCMand given in the form of concentrate mixture,  45 g DCM/kg concentrate. The feeding treatments includedPO (forage+concentrate dan P1 (forage+concentrate+DCM. At the end of feeding trial, the animals wereslaughtered and the rumen liquor were collected to analyze rumen microbes and its fermentation.  Thedata were  analyzed using a completely randomized design with 2x2 factorial models, feeding trial (PO and P1 as the first factor  and  animals as second factor (buffaloes and cattle. The results showed that nointeraction was found between the two factors on  rumen pH, NH3 concentration, partial VFA, and rumenmicrobes. There were interaction effect of  diets treatment and animal species on total VFA. Total VFAconcentration in buffaloes  with DCM supplementation was significantly higher (P<0,05 than cattle  withor without DCM supplementation. DCM supplementation decreased rumen pH and increased total VFA.The buffaloes had significantly higher total VFA, proteolitic bacteria, and butiric acid,  but  had lowerrumen pH and protozoa population compared to those of cattle. It is concluded that DCM supplementationcan increase the effectivity of   protected fish oil concentration and proportion of VFA as well as rumenmicrobial population.

  1. Fish samples as bioindicator of environmental quality: synchrotron radiation total reflection X-ray fluorescence analysis (SR-TXRF)

    Energy Technology Data Exchange (ETDEWEB)

    Vives, Ana Elisa Sirito de [Universidade Metodista de Piracicaba (UNIMEP), Santa Barbara D' Oeste, SP (Brazil). Faculdade de Engenharia, Arquitetura e Urbanismo]. E-mail: aesvives@unimep.br; Moreira, Silvana [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia, Arquitetura e Urbanismo]. E-mail: Silvana@fec.unicamp.br; Brienza, Sandra Maria Boscolo [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil). Faculdade de Ciencias Matematicas, da Natureza e de Tecnologia da Informacao]. E-mail: sbrienza@unimep.br; Zucchi, Orgheda Luiza Araujo Domingues [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas]. E-mail: olzucchi@fcfrp.usp.br; Nascimento Filho, Virgilio Franco do [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)]. E-mail: virgilio@cena.usp.br

    2005-07-01

    In this study fish were used as bioindicators of environmental contamination. The species were collected in Piracicaba River, Sao Paulo state, Brazil and the toxic elements concentrations were determined in muscle tissue and viscus (liver, intestine and stomach) by Synchrotron Radiation Total Reflection X-Ray Fluorescence Analysis (SR-TXRF). Were determined the elements Ti, Cr, Mn, Fe, Ni, Cu, Zn and Ba. The results were compared with values established by Brazilian Legislation for general food. The elements concentrations evidenced potential risk to human health and environmental quality alteration of the studied area. The measurements were realized at the 'Laboratorio Nacional de Luz Sincrotron' (LNLS) located in Campinas, Sao Paulo State, Brazil. (author)

  2. Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Yan, Li; Inamori, Ryuhei; Gui, Ping; Xu, Kai-qin; Kong, Hai-nan; Matsumura, Masatoshi; Inamori, Yuhei

    2005-01-01

    A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with oxidation-reduction potential (ORP) in the Typha latifolia constructed wetlands under three different loadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.

  3. Characterization of the Arachis (Leguminosae D genome using fluorescence in situ hybridization (FISH chromosome markers and total genome DNA hybridization

    Directory of Open Access Journals (Sweden)

    Germán Robledo

    2008-01-01

    Full Text Available Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results indicating that the D genome is positioned between the A and B genomes.

  4. Fluorescence in situ hybridizations (FISH) for the localization of viruses and endosymbiotic bacteria in plant and insect tissues.

    Science.gov (United States)

    Kliot, Adi; Kontsedalov, Svetlana; Lebedev, Galina; Brumin, Marina; Cathrin, Pakkianathan Britto; Marubayashi, Julio Massaharu; Skaljac, Marisa; Belausov, Eduard; Czosnek, Henryk; Ghanim, Murad

    2014-02-24

    Fluorescence in situ hybridization (FISH) is a name given to a variety of techniques commonly used for visualizing gene transcripts in eukaryotic cells and can be further modified to visualize other components in the cell such as infection with viruses and bacteria. Spatial localization and visualization of viruses and bacteria during the infection process is an essential step that complements expression profiling experiments such as microarrays and RNAseq in response to different stimuli. Understanding the spatiotemporal infections with these agents complements biological experiments aimed at understanding their interaction with cellular components. Several techniques for visualizing viruses and bacteria such as reporter gene systems or immunohistochemical methods are time-consuming, and some are limited to work with model organisms and involve complex methodologies. FISH that targets RNA or DNA species in the cell is a relatively easy and fast method for studying spatiotemporal localization of genes and for diagnostic purposes. This method can be robust and relatively easy to implement when the protocols employ short hybridizing, commercially-purchased probes, which are not expensive. This is particularly robust when sample preparation, fixation, hybridization, and microscopic visualization do not involve complex steps. Here we describe a protocol for localization of bacteria and viruses in insect and plant tissues. The method is based on simple preparation, fixation, and hybridization of insect whole mounts and dissected organs or hand-made plant sections, with 20 base pairs short DNA probes conjugated to fluorescent dyes on their 5' or 3' ends. This protocol has been successfully applied to a number of insect and plant tissues, and can be used to analyze expression of mRNAs or other RNA or DNA species in the cell.

  5. Validation of interphase fluorescence in situ hybridization (iFISH for multiple myeloma using CD138 positive cells

    Directory of Open Access Journals (Sweden)

    Renata Kiyomi Kishimoto

    2016-06-01

    Full Text Available ABSTRACT BACKGROUND: Multiple myeloma is a plasma cell neoplasm with acquired genetic abnormalities of clinical and prognostic importance. Multiple myeloma differs from other hematologic malignancies due to a high fraction of low proliferating malignant plasma cells and the paucity of plasma cells in bone marrow aspiration samples, making cytogenetic analysis a challenge. An abnormal karyotype is found in only one-third of patients with multiple myeloma and interphase fluorescence in situ hybridization is the most useful test for studying the chromosomal abnormalities present in almost 90% of cases. However, it is necessary to study the genetic abnormalities in plasma cells after their identification or selection by morphology, immunophenotyping or sorting. Other challenges are the selection of the most informative FISH panel and determining cut-off levels for FISH probes. This study reports the validation of interphase fluorescence in situ hybridization using CD138 positive cells, according to proposed guidelines published by the European Myeloma Network (EMN in 2012. METHOD: Bone marrow samples from patients with multiple myeloma were used to standardize a panel of five probes [1q amplification, 13q14 deletion, 17p deletion, t(4;14, and t(14;16] in CD138+ cells purified by magnetic cell sorting. RESULTS: This test was validated with a low turnaround time and good reproducibility. Five of six samples showed genetic abnormalities. Monosomy/deletion 13 plus t(4;14 were found in two cases. CONCLUSION: This technique together with magnetic cell sorting is effective and can be used in the routine laboratory practice. In addition, magnetic cell sorting provides a pure plasma cell population that allows other molecular and genomic studies.

  6. Identification and characterization of marker chromosomes, de novo rearrangements and microdeletions in 100 cases with fluorescence in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, S.M.; Liu, Y.; Papenhausen, P.R. [Roche Biomedical Labs., Research Triangle Park, NC (United States)

    1994-09-01

    Results of molecular cytogenetic analysis are presented for 100 cases in which fluorescence in situ hybridization (FISH) was used as an adjunct to standard cytogenetics. Commercially available centromeric, telomeric, chromosome painting and unique sequence probes were used. Cases were from a 12-month period (June 1993-May 1994) and included examples of sex chromosome abnormalities (8), duplications (5), de novo translocations (6), satellited (12) and non-satellited (7) markers, and microdeletion syndromes (62). Satellited marker chromosomes were evaluated using a combination of DAPI/Distamycin A staining, hybridization with a classical satellite probe for chromosome 15 and hybridization with alpha-satellite probes for chromosomes 13, 14, 21 and 22. Markers positive for the chromosome 15 probe were further evaluated using unique sequence probes for the Prader-Willi/Angelman region. Microdeletion analysis was performed for Prader-Willi/Angelman (49) and DiGeorge/VCF (13) syndromes. Seven cases evaluated for Prader-Willi/Angelman syndrome demonstrated evidence of a deletion within this region. Uniparental disomy analysis was available in cases where a deletion was not detected by FISH, yet follow-up was clinically indicated. Two cases evaluated for DiGeorge/VCF syndrome demonstrated molecular evidence of a deletion. Included in our analysis is an example of familial DiGeorge syndrome.

  7. Feasibility of using fluorescence in situ hybridization (FISH) to detect early gene changes in sputum cells from uranium miners

    Energy Technology Data Exchange (ETDEWEB)

    Neft, R.E.; Rogers, J.L.; Belinsky, S.A. [and others

    1995-12-01

    Epidemiological studies have shown that combined exposure to radon progeny and tobacco smoke produce a greater than additive or synergistic increase in lung cancer risk. Lung cancer results from multiple genetic changes over a long period of time. An early change that occurs in lung cancer is trisomy 7 which is found in 50% of non-small cell lung cancer and in the far margins of resected lung tumors. The 80% mortality associated with lung cancer is in part related to the high proportion of patients who present with an advanced, unresectable tumor. Therefore, early detection of patients at risk for tumor development is critical to improve treatment of this disease. Currently, it is difficult to detect lung cancer early while it is still amendable by surgery. Saccomanno, G. has shown that premalignant cytologic changes in sputum cells collected from uranium miners can be detected by a skilled, highly trained cytopathologist. A more objective alternative for identifying premalignant cells in sputum may be to determine whether an early genetic change such as trisomy 7 is present in these cells. Fluorescence in situ hybridization (FISH) can be used to identify cells with trisomy 7. The results of this investigation indicate that FISH may prove to be an accurate, efficient method to test at-risk individuals for genetic alterations in bronchial epithelial cells from sputum.

  8. Fishing

    Institute of Scientific and Technical Information of China (English)

    姜群山

    2002-01-01

    @@ Last Saturday my cousin (表兄) came to my home. We were very happy to see each other. We decided that the next day we went to fish. We got up very early that day. When we left home,the moon could still be seen in the sky.

  9. Phylogeny-function analysis of (meta)genomic libraries: screening for expression of ribosomal RNA genes by large-insert library fluorescent in situ hybridization (LIL-FISH)

    NARCIS (Netherlands)

    Leveau, J.H.J.; Gerards, S.; De Boer, W.; Van Veen, J.A.

    2004-01-01

    We assessed the utility of fluorescent in situ hybridization (FISH) in the screening of clone libraries of (meta)genomic or environmental DNA for the presence and expression of bacterial ribosomal RNA (rRNA) genes. To establish proof-of-principle, we constructed a fosmid-based library in Escherichia

  10. A sensitive fluorescent sensor for the detection of endogenous hydroxyl radicals in living cells and bacteria and direct imaging with respect to its ecotoxicity in living zebra fish.

    Science.gov (United States)

    Liu, Fei; Du, Juan; Song, Da; Xu, Meiying; Sun, Guoping

    2016-03-28

    We have synthesized a novel fluorescent probe, , which shown a high potential for imaging of endogenous ˙OH in living cells and various types of bacteria. In addition, it is an excellent sensor for in vivo imaging of ˙OH generated following treatment with TiO2NPs in zebra fish.

  11. Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice.

    Science.gov (United States)

    Pathak, Rupak; Koturbash, Igor; Hauer-Jensen, Martin

    2017-01-11

    Ionizing radiation (IR) induces numerous stable and unstable chromosomal aberrations. Unstable aberrations, where chromosome morphology is substantially compromised, can easily be identified by conventional chromosome staining techniques. However, detection of stable aberrations, which involve exchange or translocation of genetic materials without considerable modification in the chromosome morphology, requires sophisticated chromosome painting techniques that rely on in situ hybridization of fluorescently labeled DNA probes, a chromosome painting technique popularly known as fluorescence in situ hybridization (FISH). FISH probes can be specific for whole chromosome/s or precise sub-region on chromosome/s. The method not only allows visualization of stable aberrations, but it can also allow detection of the chromosome/s or specific DNA sequence/s involved in a particular aberration formation. A variety of chromosome painting techniques are available in cytogenetics; here two highly sensitive methods, multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY), are discussed to identify inter-chromosomal stable aberrations that form in the bone marrow cells of mice after exposure to total body irradiation. Although both techniques rely on fluorescent labeled DNA probes, the method of detection and the process of image acquisition of the fluorescent signals are different. These two techniques have been used in various research areas, such as radiation biology, cancer cytogenetics, retrospective radiation biodosimetry, clinical cytogenetics, evolutionary cytogenetics, and comparative cytogenetics.

  12. Diagnosis of a constitutional five-chromosome rearrangement by fluorescent in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Tsien, F.; Shapira, E. [Tulane Univ. School of Medicine, New Orleans, LA (United States); Carvalho, T. [Hospital Sarah Kubitschek, Brasilia (Brazil)] [and others

    1994-09-01

    Complex chromosomal rearrangements are structural rearrangements involving at least three chromosomes and three or more chromosome breakpoints. Such karyotypes are often acquired during cancer multi-step development and in chromosome instability syndromes. However, extremely rare constitutional forms have been reported, most of which are incompatible with life. We present a 2-year-old female with de novo complex rearrangement consisting of five chromosomes and nine breakpoints. Clinical evaluation at two years of age revealed a weight of 5 kg, length of 66 cm, and had circumference of 38 cm, all below the 5th percentile, microcephaly, trigonocephaly, epicanthal folds, inguinal hernia, left clubfoot, hypertonicity, and developmental delay. The neurological examination revealed chorea-acanthocytosis and psychomotor delay. Cultured lymphocytes and fibroblasts revealed a karyotype consisting of five derivative chromosomes. The metaphases were further analyzed by FISH using chromosome-specific libraries and telomeric probes in order to delineate the composition of the rearranged chromosomes; FISH results demonstrated a karyotype of: 46,XX,1pter{r_arrow}1q25::1q42.1{r_arrow}1qter, 2pter{r_arrow}q32.3::1q32.3{r_arrow}2q41::2q37.3{r_arrow}2qter, 7qter{r_arrow}7q21.2::6q22.3{r_arrow}6qter::1q31{r_arrow}1q32.3::6p23{r_arrow}6q22.3, 7pter{r_arrow}7q21.1::6p23{r_arrow}6pter, 2q33{r_arrow}2q37, 1::9p21{r_arrow}9qter. This analysis demonstrates the usefulness of FISH in characterizing complex chromosome rearrangements otherwise difficult to correctly interpret using classical cytogenetics alone.

  13. Pharmacological characterization of the oxytocic peptides in the pituitary of a marine teleost fish (pollachius virens) (1961); Caracterisation pharmacologique des peptides ocytociques dans la glande pituitaire d'un poisson marin teleost (pollachius virens) (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Maetz, J.; Morel, F. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Heller, H.; Pickering, B.T. [Bristol Univ. (United Kingdom)

    1961-07-01

    Demonstration of the existence, in the neurohypophysis of a marine fish (pollachius virens), of an oxytocic-active peptide different to the oxytocin in mammals and to the arginine vasotocin. (authors) [French] Mise en evidence dans la neurohypophyse d'un poisson marin (pollachius virens) d'un peptide a activite ocytocique different de l'ocytocine des mammiferes et de l'arginine vasotocine. (auteurs)

  14. Prediction of melting temperatures in fluorescence in situ hybridization (FISH) procedures using thermodynamic models

    DEFF Research Database (Denmark)

    Fontenete, Sílvia; Guimarães, Nuno; Wengel, Jesper

    2016-01-01

    Abstract The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA thermody......Abstract The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of one, two or more complementary nucleic acid strands, has been studied for many years. The appearance of the nearest-neighbor model led to several theoretical and experimental papers on DNA...... thermodynamics that provide reasonably accurate thermodynamic information on nucleic acid duplexes and allow estimation of the melting temperature. Because there are no thermodynamic models specifically developed to predict the hybridization temperature of a probe used in a fluorescence in situ hybridization...

  15. Identification of intracellular bacteria in adenoid and tonsil tissue specimens: the efficiency of culture versus fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Stępińska, M; Olszewska-Sosińska, O; Lau-Dworak, M; Zielnik-Jurkiewicz, B; Trafny, E A

    2014-01-01

    Monocyte/macrophage cells from human nasopharyngeal lymphoid tissue can be a source of bacteria responsible for human chronic and recurrent upper respiratory tract infection. Detection and characterization of pathogens surviving intracellularly could be a key element in bacteriological diagnosis of the infections as well as in the study on interactions between bacteria and their host. The present study was undertaken to assess the possibility of isolation of viable bacteria from the cells expressing monocyte/macrophage marker CD14 in nasopharyngeal lymphoid tissue. Overall, 74 adenotonsillectomy specimens (adenoids and tonsils) from 37 children with adenoid hypertrophy and recurrent infections as well as 15 specimens from nine children with adenoid hypertrophy, which do not suffer from upper respiratory tract infections (the control group), were studied. The suitability of immunomagnetic separation for extraction of CD14(+) cells from lymphoid tissue and for further isolation of the intracellular pathogens has been shown. The coexistence of living pathogens including Haemophilus influenzae, Staphylococcus aureus, and Streptococcus pyogenes with the bacteria representing normal nasopharyngeal microbiota inside CD14(+) cells was demonstrated. Twenty-four strains of these pathogens from 32.4 % of the lysates of CD14(+) cells were isolated. Concurrently, the fluorescent in situ hybridization (FISH) with a universal EUB388, and the species-specific probes demonstrated twice more often the persistence of these bacterial species in the lysates of CD14(+) cells than conventional culture. Although the FISH technique appears to be more sensitive than traditional culture in the intracellular bacteria identification, the doubts on whether the bacteria are alive, and therefore, pathogenic would still exist without the strain cultivation.

  16. Karyotype differentiation of four Cestrum species (Solanaceae) revealed by fluorescent chromosome banding and FISH.

    Science.gov (United States)

    Fernandes, Thiago; de Almeida Rego, Letícia do Nascimento Andrade; Nardy, Mariana; Yuyama, Priscila Mary; Vanzela, André Luís Laforga

    2009-04-01

    The karyotypes of four South American species of Cestrum (C. capsulare,C. corymbosum,C. laevigatum and C. megalophylum) were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA(+) bands and 45S rDNA were located predominantly in terminal regions. The C-CMA (+) /DAPI (+) bands appeared in interstitial and terminal regions, and the C-DAPI (+) bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species except C. capsulare, where they were found in the paracentromeric region of the long arm of pair 4. The differences in band patterns among the species studied here, along with data from other nine species reported in the literature, suggest that the bands are dispersed in an equilocal and non-equilocal manner and that structural rearrangements can be responsible for internal karyotype diversification. However, it is important to point out that the structural changes involving repetitive segments did not culminate in substantial changes in the general karyotype structure concerning chromosome size and morphology.

  17. Karyotype differentiation of four Cestrum species (Solanaceae revealed by fluorescent chromosome banding and FISH

    Directory of Open Access Journals (Sweden)

    Thiago Fernandes

    2009-01-01

    Full Text Available The karyotypes of four South American species of Cestrum (C. capsulare, C. corymbosum, C. laevigatum and C. megalophylum were studied using conventional staining, C-CMA/DAPI chromosome banding and FISH with 45S and 5S rDNA probes. The karyotypes showed a chromosome number of 2n = 2x = 16, with metacentric chromosomes, except for the eighth submeta- to acrocentric pair. Several types of heterochromatin were detected, which varied in size, number, distribution and base composition. The C-CMA+ bands and 45S rDNA were located predominantly in terminal regions. The C-CMA+/DAPI+ bands appeared in interstitial and terminal regions, and the C-DAPI+ bands were found in all chromosome regions. The 5S rDNA sites were observed on the long arm of pair 8 in all species except C. capsulare, where they were found in the paracentromeric region of the long arm of pair 4. The differences in band patterns among the species studied here, along with data from other nine species reported in the literature, suggest that the bands are dispersed in an equilocal and non-equilocal manner and that structural rearrangements can be responsible for internal karyotype diversification. However, it is important to point out that the structural changes involving repetitive segments did not culminate in substantial changes in the general karyotype structure concerning chromosome size and morphology.

  18. DAMPAK PEMBUANGAN LIMBAH TERHADAP PERUBAHAN KUALITAS OSEANOGRAFI BIOFISIK-KIMIA DAN PRODUKSI IKAN TERI (Stolephorus spp. DI PERAIRAN LAUT TELUK AMBON (The Impact of Waste Disposal on the Biophysical-chemical Characteristics Changes and Teri fish

    Directory of Open Access Journals (Sweden)

    Latif Sahubawa

    2001-04-01

    m2 pada musim Barat di wilayah Ambang Galala-Rumahtiga. Potensi ikan pelagis kecil pada musim Timur 63.968,76 tor/tahun, Peralihan 56.311,55 ton/tahun, dan Barat 60.244,35 ton/tahun atau 3,86Vo dari total potensi ikan pelagis kecil perairan Maluku (1.564,000 ton/tahun. Densitas ikan pelagis kecit pab musim Timur 34,62 kg/m3, Peralihan 29,83 kg/m3, dan Barat 32,33 kg/m3. Tingkat eksploitasi sumber daya ikan pelagis kecil perairan Teluk Ambon yaitu 30 % ("status sedang berkembang".   ABSTRACT The research objectives are: (1 identify the characteristics of waste from human activities that cause reduced biophysical-chemical oceanography characteristics of Ambon Bay Marine; (2 to evaluate the reduced biophysichemical oceanography characteristics of Ambon Bay marine in relation to assigmen requirements for fish aquaticulture; and (3 to evaluate the effect of assignment requirements fulfillment on the abundance and density of small pelagic fish, and teri fish production during Eastern and Western monsoons. Research samples consisted of sea water, teri fish, and mollusca. Sample collection methods were simple random and nonrandom sampling. Data were analyzed by using Norman Curve, Least Square, Factorial and Block completely Randomized Design with F-test, Nutritional Value Coefficient (NVC, surplus production, Hydroacoustic and Utermohl Sedimentation Methods. The results of the statistical analyses show that in general, the variables did not affect the biophysical-chemical oceanographic parameters of the Ambon Bay marine waters. The values range of the biophysical-chemical oceanography parameters of Ambon Bay marine are: temperature 23.7-28oC, TSS 2.005-12.436 mg/l, salinity 24.00-32.66 ppt, clearance 2.5-9.0 meters, pH 6.5-8.6, solved oxygen 2.09-6.88 mg/l, BOD5 10.0-50.0 mg/l, COD 22.5-150.8 mg/l, PO43- 0.22-329 µg/l, NO2- 0.22-2.94 µg/l, NO3- 0.21-15.40 µg/l, Hg in the water body 0.001-0.065 mg/l and mollusca meat 0.115-0.741 mg/l, and hydrocarbons 0.011-2.540 mg

  19. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): A Method for Bacterial Small RNA Detection

    Science.gov (United States)

    2012-01-10

    Friedrich, U. & Lenke, J. Improved Enumeration of Lactic Acid Bacteria in Mesophilic Dairy Starter Cultures by Using Multiplex Quantitative Real...messenger RNA using locked nucleic acid probes. Anal. Biochem. 390, 109-114 (2009). 13. Waters, L. & Storz, G. Regulatory RNAs in bacteria . Cell. 136, 615...Video Article Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection Kelly

  20. Fluorescence in situ hybridization and sequential catalysed reporter deposition (2C-FISH for the flow cytometric sorting of freshwater ultramicrobacteria

    Directory of Open Access Journals (Sweden)

    Stefan M Neuenschwander

    2015-03-01

    Full Text Available Flow cytometric sorting is a powerful tool to physically separate cells within mixed microbial communities. If combined with phylogenetic staining (fluorescence in situ hybridization, FISH it allows to specifically sort defined genotypic microbial populations from complex natural samples. However, the targeted enrichment of freshwater ultramicrobacteria, such as members of the LD12 clade of Alphaproteobacteria (SAR11-IIIb, is still challenging. Current FISH protocols, even in combination with signal amplification by catalysed reporter deposition (CARD, are not sufficiently sensitive for the distinction of these bacteria from background noise by flow cytometry, presumably due to their low ribosome content and small cell sizes. We, therefore, modified a CARD based flow sorting protocol with the aim of increasing its sensitivity to a level sufficient for ultramicrobacteria. This was achieved by a second signal amplification step mediated by horseradish peroxidase labelled antibodies targeted to the fluorophores that were previously deposited by CARD-FISH staining. The protocol was tested on samples from an oligo-mesotrophic lake. Ultramicrobacteria affiliated with LD12 Alphaproteobacteria could be successfully sorted to high purity by flow cytometry. The ratios of median fluorescence signal to background ranged around 20, and hybridization rates determined by flow cytometry were comparable to those obtained by fluorescence microscopy. Potential downstream applications of our modified cell staining approach range from the analysis of microdiversity within 16S rRNA-defined populations to that of functional properties, such as the taxon-specific incorporation rates of organic substrates.

  1. [Interphase cytogenetics of the breast tumors with fluorescence in situ hybridization (FISH) on cytologic preparation--its practice and clinical applications].

    Science.gov (United States)

    Shibuya, M; Osamura, R Y

    1996-05-01

    Fluorescence in situ hybridization (FISH) study with the chromosome specific probes is performed in the interphase nuclei of the routinely processed cytologic preparation of the breast tumors. Numerical aberrations on the chromosomes 1, 3, 11 or 17 were detected in more than 80% of the malignant tumors, but not in the benign tumors. Marked heterogeneity of the polysomies is noted in the malignant tumor cells. A few malignant cases revealed monosomy of chromosome 17. No apparent correlation between the numerical abnormalities and the histological features in malignant tumors is identified. These results suggest that the interphase cytogenetics with FISH for the breast tumors may be useful for differential diagnosis of malignancy. The practice and the clinical applications of the FISH study are discussed.

  2. Numbers and locations of native bacteria on field-grown wheat roots quantified by fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Watt, Michelle; Hugenholtz, Philip; White, Rosemary; Vinall, Kerry

    2006-05-01

    Native bacteria, Pseudomonas and filamentous bacteria were quantified and localized on wheat roots grown in the field using fluorescence in situ hybridization (FISH). Seminal roots were sampled through the season from unploughed soil in a conservation farming system. Such soils are spatially heterogeneous, and many roots grow slowly through hard soil with cracks and pores containing dead roots remnant from previous crops. Root and rhizosphere morphology, and contact with soil particles were preserved, and autofluorescence was avoided by observing sections in the far-red with Cy5 and Cy5.5 fluorochromes. Spatial analyses showed that bacteria were embedded in a stable matrix (biofilm) within 11 microm of the root surface (range 2-30 microm) and were clustered on 40% of roots. Half the clusters co-located with axial grooves between epidermal cells, soil particles, cap cells or root hairs; the other half were not associated with visible features. Across all wheat roots, although variable, bacteria averaged 15.4 x 10(5) cells per mm(3) rhizosphere, and of these, Pseudomonas and filaments comprised 10% and 4%, respectively, with minor effects of sample time, and no effect of plant age. Root caps were most heavily colonized by bacteria along roots, and elongation zones least heavily colonized. Pseudomonas varied little with root development and were 17% of bacteria on the elongation zone. Filamentous bacteria were not found on the elongation zone. The most significant factor to rhizosphere populations along a wheat root, however, was contact with dead root remnants, where Pseudomonas were reduced but filaments increased to 57% of bacteria (P < 0.001). This corresponded with analyses of root remnants showing they were heavily colonized by bacteria, with 48% filaments (P < 0.001) and 1.4%Pseudomonas (P = 0.014). Efforts to manage rhizosphere bacteria for sustainable agricultural systems should continue to focus on root cap and mucilage chemistry, and remnant roots as

  3. Le diagnostic anténatal de la trisomie 21 par l'hybridation in situ en fluorescence (FISH): à propos des premiers tests réalisés au Maroc

    Science.gov (United States)

    Lamzouri, Afaf; Natiq, Abdelhafid; Tajir, Mariam; Sendid, Mohamed; Sefiani, Abdelaziz

    2012-01-01

    Introduction Le but de cette étude était de présenter les premiers résultats de diagnostic anténatal de la trisomie 21 par la technique d'hybridation in situ en fluorescence (FISH) au Maroc et discuter son intérêt dans le diagnostic rapide de cette aneuploïdie. Méthodes Ce travail a été réalisé chez 23 femmes avec des grossesses à haut risque de trisomie 21. La moyenne d’âge des gestantes étaient de 37,43 ans avec des extrêmes de 21 et 43 ans. Toutes étaient musulmanes mariées, mariage légitimé par la Charia, dont trois mariages consanguins, sauf une originaire de la République Démocratique du Congo qui était chrétienne et concubine. La majorité des femmes étaient fonctionnaires et avaient un niveau de scolarisation moyen à élevé. Toutes les patientes ont bénéficié d'une consultation de génétique médicale au cours de laquelle il leur a été donné des informations sur la technique, son intérêt et ses limites. Il s'agit de femmes enceintes qui avaient soit un âge maternel élevé ou des signes d'appel échographiques et/ ou biochimiques. Une des patientes était porteuse d'une translocation robertsonienne t(14;21) équilibrée. Une amniocentèse a été réalisée chez toutes les gestantes et aucun avortement n'a était induit par ce geste invasif. L’âge gestationnel moyen à la première consultation était de 14 semaines d'aménorrhée (SA) et à l'amniocentèse était de 16 SA et 5 jours. L'analyse FISH a été réalisée, après consentement des couples, sur des cellules non cultivées à partir des échantillons de liquides amniotiques, en utilisant des sondes spécifiques du chromosome 21. Résultats Parmi les 23 patientes qui ont bénéficiées d'un diagnostic anténatal de la trisomie 21 par la technique FISH, nous avons pu rassurer 21 d'entre elles, et nous avons détecté deux cas de trisomie 21 fœtal. Conclusion La technique FISH permet un diagnostic anténatal rapide, en moins de 48h, de la trisomie 21 sur

  4. Comparison of high resolution chromosome banding and fluorescence in situ hybridization (FISH) for the laboratory evaluation of Prader-Willi syndrome and Angelman syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Delach, J.A.; Rosengren, S.S.; Kaplan, L.; Greenstein, R.M.; Cassidy, S.B.; Benn, P.A.

    1994-08-01

    The development of probes containing segments of DNA from chromosome region 15q11-q13 provides the opportunity to confirm the diagnosis of Prader-Willi syndrome (PWS) and Angelman syndrome (AS) by fluorescence in situ hybridization (FISH). We have evaluated FISH studies and high resolution chromosome banding studies in 14 patients referred to confirm or rule out AS. In four patients (three from the PWS category and 1 from the AS group) chromosome analysis suggested that a deletion was present but FISH failed to confirm the finding. In one AS group patient, FISH identified a deletion not detectable by high resolution banding. Review of the clinical findings in the discrepant cases suggested that FISH results were correct and high resolution findings were erroneous. Studies with a chromosome 15 alpha satellite probe (D15Z) on both normal and abnormal individuals suggested that incorrect interpretation of chromosome banding may occasionally be attributable to alpha satellite polymorphism but other variation of 15q11-q13 chromosome bands also contributes to misinterpretation. We conclude that patients who have been reported to have a cytogenetic deletion of 15q11-q13 and who have clinical findings inconsistent with PWS and AS should be re-evaluated by molecular genetic techniques. 31 refs., 3 figs., 2 tabs.

  5. mRNA-targeted Fluorescent in Situ Hybridization (FISH) of Gram-negative Bacteria Without Template Amplification or Tyramide Signal Amplification

    Energy Technology Data Exchange (ETDEWEB)

    Coleman, James R.; Culley, David E.; Chrisler, William B.; Brockman, Fred J.

    2007-12-01

    Technologies are needed to study gene expression at the level of individual cells within a population or microbial community. Fluorescent in situ hybridization (FISH) supplies high-resolution spatial information and has been widely applied to study microbial communities at the rRNA level. While mRNA-targeted FISH has been popular for studying gene expression in eukaryotic cells, very little success has been achieved with prokaryotes. At present, detection of specific mRNAs in individual prokaryotic cells requires the use of in situ-RT-PCR or tyramide signal amplification (TSA). In this study we used DNA oligonucleotide probes labeled with a single near-infrared dye in FISH assays to detect multicopy plasmid-based and endogenous mRNA molecules in Escherichia coli and Shewanella oneidensis MR-1. We took advantage of the fact there is much less background signal produced by biological materials and support matrices in the near-infrared spectrum and thus long camera exposure times could be used. In addition, we demonstrate that a combination of probes targeting both rRNA and mRNA could be successfully employed within the same FISH assay. These results, as well as ongoing R&D improvements in NIR and infrared dyes, indicate the FISH approach we demonstrated could be applied in certain environmental settings to monitor gene expression in mixed populations.

  6. Comparison of immunohistochemistry (IHC and fluorescence in situ hybridization (FISH assessment for Her-2 status in breast cancer

    Directory of Open Access Journals (Sweden)

    Qi Minfang

    2009-11-01

    Full Text Available Abstract Background The concordance rate between IHC and FISH according to clinical performance is still controversial. We report a prospective study to reflect the concordance between IHC and FISH in Guilin city, People's Republic of China. Methods Fifty cases of invasive ductal carcinoma of breast tested by IHC and scored as 0, 1+, 2+ and 3+ by pathologists were further analyzed by FISH using a commercially available double-color probe, and the FISH findings were compared with IHC test results. Results A total concordance of 82.0% was observed with a Kappa coefficient of 0.640 (P Conclusion The IHC can be used firstly to screen the HER-2 status, and FISH can be used as a supplementary role to IHC and 2+ and some negative cases. And only those cases with Her-2 status of IHC 3+ or FISH positive should be treated with Herceptin.

  7. Fluorescence in situ hybridization (FISH using non-commercial probes in the diagnosis of clinically suspected microdeletion syndromes

    Directory of Open Access Journals (Sweden)

    Ashutosh Halder

    2013-01-01

    Interpretation & conclusion: We conclude that FISH should not be the method of choice for clinically suspected microdeletion syndromes. We propose to follow strict clinical criteria for FISH testing or preferably to follow better methods (genotype first approach. Whole genome screening may be used as first line of test and FISH may be used for confirmation of screening result, screening of family members and prenatal diagnosis.

  8. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH)

    Institute of Scientific and Technical Information of China (English)

    Ke BI; James P.BOGART; Jinzhong FU

    2009-01-01

    The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution [Current Zoology 55(2):145-149,2009].

  9. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH

    Directory of Open Access Journals (Sweden)

    Jinzhong FU

    2009-04-01

    Full Text Available The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA. Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution.

  10. KONSUMSI IKAN LAUT KADAR MERCURY DALAM RAMBUT DAN KESEHATAN NELAYAN DI PANTAI KENJERAN SURABAYA (Sea Fish Consumption, Degree of Mercury Content in Hair, and Fisherman Health at Surabaya Kenjeran Beach, Indonesia

    Directory of Open Access Journals (Sweden)

    Sudarmaji Sudarmaji

    2004-11-01

    Full Text Available ABSTRAK Pantai Kenjeran di Surabaya mempunyai banyak fungsi baik sebagai tempat rekreasi, perikanan serta tempat pembuangan limbah dari kota Surabaya. Studi sebelumnya telah menjelaskan bahwa pantai Kenjeran telah tercemar khususnya Hg. Polutan ini telah diindikasikan terdapat dalam ikan yang dikonsumsi masyarakat sekitar. Penelitian ini mengkaji dampak mengkonsumsi ikan dari Kenjeran kaitannya dengan kesehatan masyarakat yang menkonsumsi ikan. Peneliltian ini rnengambil sample 70 orang yang mengkonsumsi ikan dan 45 orang sebagai kontrol grup. Dalarn penelitian ini rambut responden diambil dan dikaji dengan Atomic Absorption Spectrophotometer (AAS. Penelitian ini menghasilkan bahwa responden yang mengkonsumsi ikan sebanyak rata-rata 99,11 gram/hari mempunyai kadar Hg dalam rambutnya sebesar 0.511 ppb. Penelitian ini mengindikasikan gejala-gejala penyakit yang terjadi pada mereka yang rnengkonsumsi ikan antara lain ginjal, pusing-pusing, tumor, pendarahan gusi, dan gangguan penglihatan. Penelitian ini rnenyimpulkan adanya korelasi yang signifikan antara responden yang mengkonsumsi ikan yang tercemar dengan kadar Hg dalam rambutnya.   ABSTRACT Surabaya Kenjeran Beach, as a part of Eastern coastal area at East Java, functions as a sea recreation place and fishing. The condition of Surabaya Kenderan Beach is polluted by Hg as observed by previous researchers. They suggested that water, sediment, and fish from Kenjeran beach were already contaminated by Hg at dangerous level. Fisherman communities is one of the group which have a risk of getting affected by methyl Hg, because they usually consume fish from sea. This research is to study the relationship between consumption of sea fish and degree of Hg in fisherman’s hair, to measure the average degree of Hg in their hair and then to compare it with limit value. It is also studying the health disorder that likely appears as a result of Hg poisoning. This research took place at Kenjeran district

  11. Fluorescence in situ hybridization (FISH screening for the 22q11.2 deletion in patients with clinical features of velocardiofacial syndrome but without cardiac anomalies

    Directory of Open Access Journals (Sweden)

    Paula Sandrin-Garcia

    2007-01-01

    Full Text Available The velocardiofacial syndrome (VCFS, a condition associated with 22q11.2 deletions, is characterized by a typical facies, palatal anomalies, learning disabilities, behavioral disturbances and cardiac defects. We investigated the frequency of these chromosomal deletions in 16 individuals with VCFS features who presented no cardiac anomalies, one of the main characteristics of VCFS. Fluorescent in situ hybridization (FISH with the N25 (D22S75; 22q11.2 probe revealed deletions in ten individuals (62%. Therefore, even in the absence of cardiac anomalies testing for the 22q11.2 microdeletions in individuals showing other clinical features of this syndrome is recommended.

  12. Phylogeny-function analysis of (meta)genomic libraries: screening for expression of ribosomal RNA genes by large-insert library fluorescent in situ hybridization (LIL-FISH).

    Science.gov (United States)

    Leveau, Johan H J; Gerards, Saskia; de Boer, Wietse; van Veen, Johannes A

    2004-09-01

    We assessed the utility of fluorescent in situ hybridization (FISH) in the screening of clone libraries of (meta)genomic or environmental DNA for the presence and expression of bacterial ribosomal RNA (rRNA) genes. To establish proof-of-principle, we constructed a fosmid-based library in Escherichia coli of large-sized genomic DNA fragments of the mycophagous soil bacterium Collimonas fungivorans, and hybridized 768 library clones with the Collimonas-specific fluorescent probe CTE998-1015. Critical to the success of this approach (which we refer to as large-insert library FISH or LIL-FISH) was the ability to induce fosmid copy number, the exponential growth status of library clones in the FISH assay and the use of a simple pooling strategy to reduce the number of hybridizations. Twelve out of 768 E. coli clones were suspected to harbour and express Collimonas 16S rRNA genes based on their hybridization to CTE998-1015. This was confirmed by the finding that all 12 clones were also identified in an independent polymerase chain reaction-based screening of the same 768 clones using a primer set for the specific detection of Collimonas 16S ribosomal DNA (rDNA). Fosmids isolated from these clones were grouped by restriction analysis into two distinct contigs, confirming that C. fungivorans harbours at least two 16S rRNA genes. For one contig, representing 1-2% of the genome, the nucleotide sequence was determined, providing us with a narrow but informative view of Collimonas genome structure and content.

  13. PARAFAC modeling of fluorescence excitation - Emission spectra of fish bile for rapid en route screening of PAC exposure

    DEFF Research Database (Denmark)

    Christensen, Jan H.; Tomasi, Giorgio; Strand, Jakob

    2009-01-01

    with parallel factor analysis (PARAFAC) and may constitute an alternative to fixed wavelength fluorescence and synchronous fluorescence spectroscopy (M). PARAFAC was applied to excitation-emission matrices (EEMs) of bile samples of shorthorn sculpins and European eels collected in Greenland and Denmark...

  14. [Evaluation of peptide nucleic acid fluorescent in situ hybridization (PNA FISH) method in the identifi cation of Candida species isolated from blood cultures].

    Science.gov (United States)

    Aydemir, Gonca; Koç, Ayşe Nedret; Atalay, Mustafa Altay

    2016-04-01

    In recent years, increased number of patients who are hospitalized in intensive care units, received immunosuppressive therapy and treated with broad-spectrum antibiotics that can lead an increase in the incidence of systemic candidiasis. In these patients, the most common clinical manifestation is candidemia. Since the identification of Candida species isolated from blood cultures is time consuming by conventional (morphological and biochemical) methods, rapid, reliable and accurate methods are needed. For this purpose novel systems have been developed to identify the agent directly. The aim of this study was to evaluate the peptide nucleic acid fluorescent in situ hybridization (PNA FISH) method for the identification of Candida species by comparing with the conventional methods. A total of 50 patients who were admitted to Erciyes University Medical Faculty Hospital clinics and followed with prediagnosis of systemic fungal infections whose blood cultures were positive for the yeasts between July 2011 and July 2012 were included in the study. The conventional identification of Candida isolates was performed by considering macroscopic and microscopic morphology, germ tube test, cycloheximide sensitivity, urease activity and carbohydrate assimilation patterns with API 20C AUX (bioMerieux, France) test. PNA FISH method was conducted by the use of a commercial kit namely Yeast Traffic Light(®) PNA FISH (AdvanDx, USA). According to morphological and biochemical characteristics (conventional methods), 19 (38%) out of 50 Candida isolates were identified as C.albicans, 12 (24%) as C.glabrata, five (10%) as C.parapsilosis, five (10%) as C.kefyr, four (8%) as C.krusei, two (4%) as C.guilliermondii, two (4%) as C.tropicalis and one (2%) as C.lusitaniae. On the other hand, 24 (48%) of the isolates were identified as C.albicans/C.parapsilosis (with green fluorescence), 16 (32%) as C.glabrata/C.krusei (with red fluorescence) and one (%2) as C.tropicalis (with yellow

  15. Resolving colocalization of bacteria and metal(loid)s on plant root surfaces by combining fluorescence in situ hybridization (FISH) with multiple-energy micro-focused X-ray fluorescence (ME μXRF).

    Science.gov (United States)

    Honeker, Linnea K; Root, Robert A; Chorover, Jon; Maier, Raina M

    2016-12-01

    Metal(loid)-contamination of the environment due to anthropogenic activities is a global problem. Understanding the fate of contaminants requires elucidation of biotic and abiotic factors that influence metal(loid) speciation from molecular to field scales. Improved methods are needed to assess micro-scale processes, such as those occurring at biogeochemical interfaces between plant tissues, microbial cells, and metal(loid)s. Here we present an advanced method that combines fluorescence in situ hybridization (FISH) with synchrotron-based multiple-energy micro-focused X-ray fluorescence microprobe imaging (ME μXRF) to examine colocalization of bacteria and metal(loid)s on root surfaces of plants used to phytostabilize metalliferous mine tailings. Bacteria were visualized on a small root section using SytoBC nucleic acid stain and FISH probes targeting the domain Bacteria and a specific group (Alphaproteobacteria, Gammaproteobacteria, or Actinobacteria). The same root region was then analyzed for elemental distribution and metal(loid) speciation of As and Fe using ME μXRF. The FISH and ME μXRF images were aligned using ImageJ software to correlate microbiological and geochemical results. Results from quantitative analysis of colocalization show a significantly higher fraction of As colocalized with Fe-oxide plaques on the root surfaces (fraction of overlap 0.49±0.19) than to bacteria (0.072±0.052) (pbacteria that colocalized with metal(loid)s, Actinobacteria, known for their metal tolerance, had a higher correlation with both As and Fe than Alphaproteobacteria or Gammaproteobacteria. This method demonstrates how coupling these micro-techniques can expand our understanding of micro-scale interactions between roots, metal(loid)s and microbes, information that should lead to improved mechanistic models of metal(loid) speciation and fate. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. KERAGAMAN JENIS IKAN DAN KEBIASAAN MAKAN DI MUARA SUNGAI MUSI

    Directory of Open Access Journals (Sweden)

    Eko Prianto

    2016-03-01

    Full Text Available Populasi ikan di suatu perairan berkaitan erat dengan keragaman jenis dan makanan yang tersedia. Penelitian ini bertujuan untuk mengetahui komposisi jenis dan kelimpahan sumberdaya ikan berdasarkan kebiasaan makannya di muara Sungai Musi Sumatera Selatan. Pengambilan sampel dengan menggunakan pukat hela dan belad dilakukan pada bulan Maret dan Juni 2008 masing-masing pada 4 stasiun pengambilan contoh yang mewakili perairan muara Sungai Musi. Hasil penelitian diperoleh sebanyak 34 jenis ikan di bagian tepi sungai dan 63 jenis di bagian tengah sungai. Ditinjau dari kebiasaan makan pada bulan Maret untuk ikan yang berada di tepi sungai diperoleh kelimpahan ikan herbivora paling tinggi (49 % dan terendah ikan yang bersifat omnivora (1 %. Pada bulan Juni kelimpahan ikan carnivora paling tinggi (50 % dan terendah ikan omnivora (1 %. Untuk jenis ikan yang berada di tengah sungai, kelimpahan ikan yang tertinggi pada bulan Maret yaitu ikan herbivora (87 % dan terendah ikan omnivora (2 %. Pada bulan Juni kelimpahan yang tertinggi yaitu ikan carnivora (57 % dan terendah ikan omnivora (0,2 %.   Fish populations in the waters is closely related to species diversity and food available. The objective of this study is to determine of species composition and abundance of fish resources based on feeding habit in the estuarin water of Musi river. Sample was taken from 4 fishing stations by using trawl and towing net (belad in March and June 2008. The results showed that there were 34 species of fish in the riverside and 63 species of fish in the middle stream. Analysis of feeding habit in March for the fishes in found riverside showed that herbivorous fish was highest 49% of total catch and the lowest was omnivorous fish (1%. While in June the carnivorous fish was highest (50% and the lowest was omnivorous fish (1%. In the middlestream the highest abundance in March was herbivorous fish (87% and the lowest (57% was omnivorous fish (2%. In June, the highest

  17. Automated In-Injector Derivatization Combined with High-Performance Liquid Chromatography-Fluorescence Detection for the Determination of Semicarbazide in Fish and Bread Samples.

    Science.gov (United States)

    Wang, Yinan; Chan, Wan

    2016-04-06

    Semicarbazide (1) is a widespread genotoxic food contaminant originating as a metabolic byproduct of the antibiotic nitrofurazone used in fish farming or as a thermal degradation product of the common flour additive azodicarbonamide. The goal of this study is to develop a simple and sensitive high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) method for the detection of compound 1 in food products. In comparison to existing methods for the determination of compound 1, the reported method combining online precolumn derivatization and HPLC-FLD is less labor-intensive, produces higher sample throughput, and does not require the use of expensive analytical instruments. After validation of accuracy and precision, this method was applied to determine the amount of compound 1 in fish and bread samples. Comparative studies using an established liquid chromatography coupled with tandem mass spectrometry method did not yield systematically different results, indicating that the developed HPLC-FLD method is accurate and suitable for the determination of compound 1 in fish and bread samples.

  18. Fluorescence In Situ Hybridization (FISH-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris and Relatives

    Directory of Open Access Journals (Sweden)

    Aiko Iwata-Otsubo

    2016-04-01

    Full Text Available Fluorescence in situ hybridization (FISH-based karyotyping is a powerful cytogenetics tool to study chromosome organization, behavior, and chromosome evolution. Here, we developed a FISH-based karyotyping system using a probe mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and chromosome-specific BAC clones in common bean, which enables one to unambiguously distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping system to several wild relatives and landraces of common bean from two distinct gene pools, as well as other related Phaseolus species, to investigate repeat evolution in the genus Phaseolus. Comparison of karyotype maps within common bean indicates that chromosomal distribution of the centromeric and subtelomeric satellite repeats is stable, whereas the copy number of the repeats was variable, indicating rapid amplification/reduction of the repeats in specific genomic regions. In Phaseolus species that diverged approximately 2–4 million yr ago, copy numbers of centromeric repeats were largely reduced or diverged, and chromosomal distributions have changed, suggesting rapid evolution of centromeric repeats. We also detected variation in the distribution pattern of subtelomeric repeats in Phaseolus species. The FISH-based karyotyping system revealed that satellite repeats are actively and rapidly evolving, forming genomic features unique to individual common bean accessions and Phaseolus species.

  19. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH).

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I; Ortíz-Hernández, Brenda L; Dávila-Rodríguez, Martha I; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2013-02-19

    We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  20. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I.; Ortíz-Hernández, Brenda L.; Dávila-Rodríguez, Martha I.; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2013-01-01

    We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1. PMID:23429197

  1. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH

    Directory of Open Access Journals (Sweden)

    Jaime Gosálvez

    2013-02-01

    Full Text Available We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH. A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL, 10 with high-grade SIL (HG-SIL, and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  2. Nitrifying bacterial communities in an aquaculture wastewater treatment system using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and phylogenetic analysis.

    Science.gov (United States)

    Paungfoo, Chanyarat; Prasertsan, Poonsuk; Burrell, Paul C; Intrasungkha, Nugul; Blackall, Linda L

    2007-07-01

    Aquaculture, especially shrimp farming, has played a major role in the growth of Thailand's economy in recent years, as well as in many South East Asian countries. However, the nutrient discharges from these activities have caused adverse impacts on the quality of the receiving waterways. In particular nitrogenous compounds, which may accumulate in aquaculture ponds, can be toxic to aquatic animals and cause environmental problems such as eutrophication. The mineralization process is well known, but certain aspects of the microbial ecology of nitrifiers, the microorganisms that convert ammonia to nitrate, are poorly understood. A previously reported enrichment of nitrifying bacteria (ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) from a shrimp farm inoculated in a sequencing batch reactor (SBR) was studied by molecular methods. The initial identification and partial quantification of the nitrifying bacteria (AOB and NOB) were carried out by fluorescence in situ hybridization (FISH) using previously published 16S rRNA-targeting oligonucleotide probes. The two dominant bacterial groups detected by FISH were from the Cytophaga-Flavobacterium-Bacteroides and Proteobacteria (beta subdivision) phyla. Published FISH probes for Nitrobacter and Nitrospira did not hybridize to any of the bacterial cells. Therefore it is likely that new communities of NOBs, differing from previously reported ones, exist in the enrichments. Molecular genetic techniques (cloning, sequencing, and phylogenetic analysis) targeting the 16S rRNA genes from the nitrifying enrichments were performed to identify putative AOBs and NOBs.

  3. Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer : A Validation Study

    NARCIS (Netherlands)

    van der Logt, Elise M. J.; Kuperus, Deborah A. J.; van Setten, Jan W.; van den Heuvel, Marius C.; Boers, James. E.; Schuuring, Ed; Kibbelaar, Robby E.

    2015-01-01

    HER2 assessment is routinely used to select patients with invasive breast cancer that might benefit from HER2-targeted therapy. The aim of this study was to validate a fully automated in situ hybridization (ISH) procedure that combines the automated Leica HER2 fluorescent ISH system for Bond with su

  4. A water soluble and fast response fluorescent turn-on copper complex probe for H2S detection in zebra fish.

    Science.gov (United States)

    Palanisamy, Sathyadevi; Lee, Lu-Ying; Wang, Yu-Liang; Chen, Yu-Jen; Chen, Chiao-Yun; Wang, Yun-Ming

    2016-01-15

    According to the displacement method, herein we reported a water soluble copper complex [Cu(MaT-cyclen)2] as a fluorescent probe for the detection of H2S. For this, 1-((1-((10-methylanthracen-9-yl)methyl)-1H-1,2,3-triazol-4-yl)methyl)-1,4,7,10-tetraazacyclododecane (MaT-cyclen) was synthesized first. To improve its solubility in aqueous media, sodium acetate group was introduced into 8-hydroxy-2-quinoline successfully. MaT-cyclen was chelated with Cu(II) to form [Cu(MaT-cyclen)2] complex, which displayed high sensitivity and selectivity for H2S over the other possible competitive substances on the basis of forming CuS. Meanwhile, [Cu(MaT-cyclen)2] displayed rapid response (zebra fish.

  5. ANALISIS DAN PREDIKSI BEBAN PENCEMARAN LIMBAH CAIR PABRIK PENGALENGAN IKAN (Analysis and Prediction of Liquid Waste Pollutant Level in Fish Canning Plant

    Directory of Open Access Journals (Sweden)

    Latif Sahubawa

    2011-03-01

    Full Text Available ABSTRAK Penelitian bertujuan untuk mengetahui kadar parameter pencemaran serta beban pencemaran limbah cair pabrik pengalengan ikan sardin. Manfaatnya adalah sebagai saran pertimbangan kepada industri untuk mengelola limbah cair secara profesional, menjamin kelestarian dan peruntukkan badan air penerima limbah cair, serta bahan informasi ilmiah kepada pengambil kebijakan (terutama pejabat daerah dalam pengelolaan kualitas lingkungan hidup secara berkelanjutan. Metode analisis yang digunakan adalah neraca masa, dengan parameter pengamatan yaitu: debit limbah cair maksimum (DM dan debit limbah cair sebenarnya (DA, serta beban pencemaran maksimum (BPM dan beban pencemaran sebenarnya (BPA dari parameter pH, TSS, BOD, COD, dan minyak/lemak sesuai Kepmen LH. No. 06 Tahun 2007. Berdasarkan hasil pengukuran, tercatat debit air limbah aktual (Dp = 10,0 liter/detik, debit air limbah sebenarnya (DA = 2.880 m 3, serta debit air limbah maksimum (DM = 720 m 3, (jadi DA > DM. Dari hasil analisis laboratorium, kadar parameter indikator pencemaran limbah cair pabrik ikan kaleng, masing-masing: pH = 6,5; TSS = 250 mg/l; BOD 5 = 95,0 mg/l; COD = 105 mg/l; dan minyak/lemak = 0,5 mg/l. Berdasarkan hasil perhitungan, ternyata beban pencemaran sebenarnya dari masing-masing parameter (BPA-TSS, BOD 5 , dan COD lebih besar dari beban pencemaran maksimum (BPM, kecuali lemak di mana BPA MWD. Based on the laboratory analytical result, wastewater pollution indicator parameters are, respectively, pH=6.5; TSS=250 mg/l; BOD­5=95.0 mg/l; COD=105 mg/l; and oil/fat = 0.5 mg/l. Based on the measuring result, the substantive pollution load, respectively, higher than maximum pollution load (SPL>MPL, except fat parameter, while SPL less than MPL.  We can conclude that wastewater pollution load produced by canning industries, exceeded the allowed limit of wastewater quality for fisheries industries (Kepmen LH No. 06 Year 2007, and also resulted pollution (changing the utilization in

  6. Simultaneous 16S and 18S rRNA fluorescence in situ hybridization (FISH) on LR White sections demonstrated in Vestimentifera (Siboglinidae) tubeworms.

    Science.gov (United States)

    Schimak, Mario P; Toenshoff, Elena R; Bright, Monika

    2012-02-01

    Traditional morphological identification of invertebrate marine species is limited in early life history stages for many taxa. In this study, we demonstrate, by example of Vestimentiferan tubeworms (Siboglinidae, Polychaeta), that the simultaneous fluorescence in situ hybridization (FISH) of both eukaryotic host and bacterial symbiont cells is possible on a single semi-thin (1 μm) section. This allows the identification of host specimens to species level as well as offering visualization of bacteria distributed within the host tissue. Previously published 18S rRNA host-specific oligonucleotide probes for Riftia pachyptila, Tevnia jerichonana and a newly designed Oasisia alvinae probe, as well as a 16S rRNA probe targeting symbionts found in all host species, were applied. A number of standard fixation and hybridization parameters were tested and optimized for the best possible signal intensity and cellular resolution. Ethanol conserved samples embedded in LR White low viscosity resin yielded the best results with regard to both signal intensity and resolution. We show that extended storage times of specimens does not affect the quality of signals attained by FISH and use our protocol to identify morphologically unidentifiable tubeworm individuals from a small data set, conforming to previous findings in succession studies of the Siboglinidae family.

  7. Phenotypic consequences of a mosaic marker chromosome identified by fluorescence in situ hybridization (FISH) as being derived from chromosome 16

    Energy Technology Data Exchange (ETDEWEB)

    Ray, J.H.; Zhou, X.; Pletcher, B.A. [Cornell Univ. Medical College, Manhasset, NY (United States)] [and others

    1994-09-01

    De novo marker chromosomes are detected in 1 in 2500 amniotic fluid samples and are associated with a 10-15% risk for phenotypic abnormality. FISH can be utilized as a research tool to identify the origins of marker chromosomes. The phenotypic consequences of a marker chromosome derived from the short arm of chromosome 16 are described. A 26-year-old woman underwent amniocentesis at 28 weeks gestation because of a prenatally diagnosed tetralogy of Fallot. Follow-up ultrasounds also showed ventriculomegaly and cleft lip and palate. 32 of 45 cells had the karyotype 47,XY,+mar; the remaining cells were 46,XY. The de novo marker chromosome was C-band positive and non-satellited and failed to stain with distamycin A/DAPI. At birth the ultrasound findings were confirmed and dysmorphic features and cryptorchidism were noted. Although a newborn blood sample contained only normal cells, mosaicism was confirmed in 2 skin biopsies. FISH using whole-chromosome painting and alpha-satellite DNA probes showed that the marker chromosome had originated from chromosome 16. As proximal 16q is distamycin A/DAPI positive, the marker is apparently derived from proximal 16p. At 15 months of age, this child is hypotonic, globally delayed and is gavage-fed. His physical examination is significant for microbrachycephaly, a round face, sparse scalp hair, ocular hypertelorism, exotropia, a flat, wide nasal bridge and tip, mild micrognathia, and tapered fingers with lymphedema of hands and feet. Inguinal hernias have been repaired. His features are consistent with those described for patients trisomic for most or all of the short arm of chromosome 16. Marker chromosomes derived from the short arm of chromosome 16 appear to have phenotypic consequences. As the origin of more marker chromosomes are identified using FISH, their karyotype/phenotype correlations will become more apparent, which will permit more accurate genetic counseling.

  8. PERUBAHAN KARAKTERISTIK SURIMI IKAN CUCUT DAN IKAN PARI AKIBAT PENGARUH PENGKOMPOSISIAN DAN PENYIMPANAN DINGIN DAGING LUMAT [Characteristic Changes of Shark and Stingray Surimi as Affected by Compositioning and Chill Storage of the Mince Fish

    Directory of Open Access Journals (Sweden)

    Joko Santoso1

    2008-06-01

    Full Text Available This experiment was carried out to study the effects of leaching, compositioning, and chill storage of mince fish on the characteristic changes of surimi from shark and stingray fish. Three times leaching of mince fish could reduce the urea contents of the shark and stingray as much as 88% and 100%, respectively; with the salt soluble protein contents were 13.52% and 13.24%, respectively. Mixture of mince shark and stingray in proportion of 25% : 75% (A1B2 gave the highest value of gel strength being 209.29 g.cm in comparison with others composition. During chill storage, deterioration process still occurred as indicated by increasing value of acidity and contents of base volatile compounds i.e. total volatile base nitrogen (TVBN and tri-methyl amine (TMA; and also decreasing contents of urea and salt soluble protein. Deterioration process of mince fish also affected the physical characteristic of surimi i.e. decreasing values of gel strength, water holding capacity (WHC, and colour (whiteness.

  9. Caractérisation de l'état chimique d'un élément par fluorescence X : l'aluminium dans les zéolithes Characterizing the Chemical State of an Element by X-Ray Fluorescence: Aluminium in Zeolites

    Directory of Open Access Journals (Sweden)

    Freund E.

    2006-11-01

    Full Text Available La fluorescence X est essentiellement connue dans le domaine de l'analyse quantitative, mais c'est aussi une méthode qui permet d'obtenir des informations détaillées sur la structure électronique des éléments. Dans cet article, nous montrons qu'il est possible de mettre au point une technique simple de caractérisation de l'état chimique d'un élément en utilisant de façon conjointe un spectromètre commercial et un programme adapté de traitement des données. Nous avons appliqué cette technique au cas de l'aluminium dans une zéolithe particulière : la mordénite dite à petits pores modifiée par différents traitements. Nous avons ainsi pu classer les solides suivant leur rapport (AI hexa-coordinné/AI tétracoordinné et montrer que le débouchage de la mordénite dite à petits pores est lié à l'extraction d'atomes d'aluminium de la charpente cristalline. X-ray fluorescence is mainly known in the field of qua-titative analysis, but it is also a useful method for obtaining detailed informations about the electronic structure of elements. This article shows that it is possible to develop a simple technique for characterizing the chemical state of an element by combining a commercial spectrometer with a program suited for data processing. This technique has been applied to the case of aluminum in a specific zeolite, ( small portmodernite, modified by various treatments. We were thus able to classify the solids according to the ratio of hexacoordinated AI/tetracoordinated AI and to show that the deblocking of the small portmordenite is linked to the extraction of aluminum atomrs from the crystalline structure.

  10. KARAKTERISASI PROTEIN MIOFIBRIL DARI IKAN KUNIRAN (Upeneus moluccensis DAN IKAN MATA BESAR (Selar crumenophthalmus [Characterization of Myofibrillar Protein from Goldband Goat Fish (Upeneus moluccensis and Bigeye Scad Fish (Selar crumenophthalmus

    Directory of Open Access Journals (Sweden)

    Achmad Subagio

    2004-04-01

    Full Text Available Characteristics of myofibrillar protein from goldband goat fish (U. moluccensis and bigeye scad fish (S. crumenophthalmus were studied for their development as food ingredient. Color analysis using chromameter showed that myfibrillar protein from goldband goat fish was light colored, while that of bigeye scad was slightly drak colored. Proximate analysis showed that their contents were similar by crude protein 7-10%, crude fat 0.2-0.5%, and ash 0.4-0.7%. Amino acid compositions of both myofibrillar proteins were very close, dominated by glutamic acid (20%, aspartic acid (10% and lysine (9%. However, comparing with bigeye scad, myofibrillar proteins from goldband goatfish were easily aggregated, had higher gelation capacity and higher emulsion activity, but lower solubility. Based on these result, myofibrillar protein from goldband goatfish has good charachteristics as food ingredient especially for restructured products comparing with bigeye scad

  11. Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio using a green fluorescent protein (GFP and growth hormone cDNA transgene co-injection strategy

    Directory of Open Access Journals (Sweden)

    Márcio de Azevedo Figueiredo

    2007-01-01

    Full Text Available In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluated the degree of in vivo mosaicism using a strategy where a reporter transgene is co-injected with a transgene of interest so that potential germline founders can be easily identified. Transgenic zebrafish (Danio rerio were produced using two transgenes, both comprised of the carp beta-actin promoter driving the expression of either the green fluorescent protein (GFP reporter gene or the growth hormone cDNA from the marine silverside fish Odonthestes argentinensis. The methodology applied allowed a rapid identification of G0 transgenic fish and also detected which fish were transmitting transgenes to the next generation. This strategy also allowed inferences to be made about genomic transgene integration events in the six lineages produced and allowed the identification of one lineage transmitting both transgenes linked on the same chromosome. These results represent a significant advance in the reduction of the effort invested in producing a stable genetically modified fish lineage.

  12. Application of analysis by spectrometry of X-fluorescence radiation. Determination of P-W-Zr in phospho tungstate, zirconium tungstate, zirconium phosphate, their mixtures, and their degradation products; Application de l'analyse par spectrometrie du rayonnement de fluorescence X. Dosage de P - W - Zr dans les phosphotungstate, tungstate de zirconium, phosphate de zirconium, leurs melanges et leurs produits de degradation

    Energy Technology Data Exchange (ETDEWEB)

    Guillon, A.; Lebrun, M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-07-01

    By chemical methods it is possible to dose accurately the constituents of solid samples containing essentially P, Zr and T. These methods involve prior separation of the elements, an operation which is relatively lengthy and delicate; they are furthermore ill-adapted for large series. In this case, the use of the X-fluorescence technique has many advantages. The process used is the borax-bead method. The fusion eliminates heterogeneity effects such as those dus to the size, the porosity and the composition of the grains, inter-elements effects, however, are not suppressed. These effects have been studied here and corrected for. (author) [French] Les methodes chimiques permettent le dosage precis des constituents d'echantillons solides contenant essentiellement P, Zr, W. Elles necessitent la separation prealable des elements, operation relativement longue et delicate, et se pretent mal a l'analyse de series importantes. dans ce cas, l'utilisation de la technique de fluorscence X ne presente que des avantages. La methode utilisee est celle des perles au borax. La fusion efface les effets d'heterogeneite telles que la taille, la porosite et la composition des grains. Les effets inter-elements, par contre, ne sont pas supprimes. Ce travail les met en evidence et permet leur correction. (auteur)

  13. Petroleum hydrocarbons, fluorescent aromatic compounds in fish bile and organochlorine pesticides from areas surrounding the spill of the Kab121 well, in the Southern Gulf of Mexico: a case study.

    Science.gov (United States)

    Gold-Bouchot, G; Ceja-Moreno, V; Chan-Cocom, E; Zapata-Perez, O

    2014-01-01

    In October 2007, a light crude oil spill took place in the off shore Kab121 oil well, 32 km north of the mouth of the Grijalva River, Tabasco, Mexico. In order to estimate the possible effects of oil spill on the biota in the area surrounding the spilled well, the level of different fractions of petroleum hydrocarbons were measured in fish, as well as the concentration of some chlorinated hydrocarbons and PCBs. The organisms examined were cat fish (Ariopsis felis), in addition fluorescent aromatic compounds in bile, the contaminants above mentioned and their relationship with cyotochrome P-450 and Ethoxyresorufin-O-deethylase, Glutathion-S-Transferase and catalase activities in liver were determined. The concentration of most pollutants were low, except PAHs. Spatial distribution of these compounds, as well as most biomarkers, reflected the highest exposure of fish to pollutants in the area adjacent to well, as well as in the proximity of rivers. The profile of exposure to this environment was chronic in nature and not temporary.

  14. KARAKTERISTIK SUMBERDAYA IKAN DAN STRATEGI PENGELOLAAN PERIKANAN PERAIRAN SUNGAI YANG BERMUARA KE PANTAI BARAT SUMATERA

    Directory of Open Access Journals (Sweden)

    Husnah Husnah

    2012-11-01

    Manna dan Suoh di Sungai Semangka. Alternatif pengelolaan lainnya adalah peningkatan produksi ikan Semah melalui restocking induk ataupun benih. Fisheries management practices such as allocation of fish resources utilization and permitted total fish catch in marine and inland waters relate to the characteristic of fish resources. Geomorphplogical condition as well as fish resources and fisheries of rivers flow through to the western Sumatra estuary such as Manna River and Semangka River are different from the rivers flow through to eastern Sumatra estuary. Information on characteristic and management of fish resources in these rivers is limited while there is indication declining fish catch in these rivers. This paper discribed characteristic and management of Manna and Semangka rivers based on a study conducted in 2011. Rivers flow through to the western part of Sumatra estuary are characterized by constrained shape rivers, low habitat and fish diversity, low fish yield, subsistence fisheries and with two important economical fish such as carps (Tor sp and eels (Anguilla sp. In addition to forest clearance at the upper stream, population of these fish were mostly threaten by illegal fishing such us application of electro fishing and poison. Desimination of about prohibition operation of unfriendly fishing gears has been conducted by the local government, however it does not followed by implementing the law enforcement. The fisheries management of Manna and Semangka rivers should be based on the integration and responsible principle and should be focused on the habitat and population of the two economical fish. The alternative fisheries management strategies are in situ conservation such as establishment of fisheries reserves situated at the river pools specially the site area with abundant fish fries and fish brooders such as in Air Tenam Manna River and Suoh in Semangka River. Enhancing of fish stock through restocking fish at fry and brooder size is other management

  15. International, collaborative assessment of limitations of chromosome-specific probes (CSP) and fluorescent in situ hybridization (FISH): Analysis of expected detections in 73,000 prenatal cases

    Energy Technology Data Exchange (ETDEWEB)

    Evans, M.I.; Henry, G.P.; Miller, W.A. [Wayne State Univ., Detroit, MI (United States)] [and others

    1994-09-01

    FISH and CSP have been proposed to reduce karyotyping need. The purpose of this study was to assess the potential efficacy of CSP-FISH using currently available probes (13, 18, 21, X, & Y) in large, prenatal diagnostic centers. Results (1990-1993) from 7 centers in 4 countries were divided by those expected to be detectable by currently available probes, and those which would be missed assuming 10% probe efficacy. 72,994 karyotypes included 699 trisomy 21`s, 352 trisomy 18`s, 136 trisomy 13`s, 358 sex chromosome aneuploidies, 70 triploidies, and 855 others (translocations, inversions, deletions, markers). Of 2,613 abnormalities, 1,745 would be detectable (66.8%). [Detroit 55.7%, Stockholm 68.3%, Boston 52.6%, Denver 61.3%, Muenster 77.0%, London 84.5%, Philadelphia 69.4%]. Centers with high proportions of referrals for ultrasound anomalies had the highest CSP-FISH positives secondary to increased T 18 & 13. We conclude: (1) 73,000 karyotypes show relatively consistent incidences of the common trisomies, sex chromosome abnormalities, and other chromosome abnormalities among the centers. (2) The proportion expected detectable by FISH-CSP technology varies from 52.6% to 84.5%, averaging 66.8%. (3) 1/3 of the karyotypic abnormalities would be missed, and therefore, replacement of complete karyotyping with FISH would have unacceptably high false-negative rates for routine evaluation. (4) FISH-CSP, while useful when positive for anomalies, is not sufficient when negative to obviate the need for a complete karyotype.

  16. Role of immunohistochemistry and fluorescence in-situ hybridization (FISH in the diagnosis of spindle and round cell tumors of the kidney

    Directory of Open Access Journals (Sweden)

    M. Abbas

    2015-09-01

    Conclusion: In summary we advise an immunohistochemical panel for round/spindle cell tumors of the kidney and for unclear cases we advise to add (FISH to get the correct diagnosis, as they are completely different regarding surgical approach and post-operative adjuvant therapy.

  17. 荧光原位杂交法(FISH)快速检测尿液中金黄色葡萄球菌%Fluorescence in Situ Hybridization Rapidly Detects Staphylococcus Aureus in Urinary Tract Infection Samples

    Institute of Scientific and Technical Information of China (English)

    吴青; 李艳; 汪明; 顾剑; 胡慧霞; 徐万洲; 韦传东; 吴泽刚

    2011-01-01

    目的 利用荧光原位杂交法快速检测尿液中的金黄色葡萄球菌,筛查金黄色葡萄球菌所致的尿路感染.方法 针对金黄色葡萄球菌16sRNA设计荧光标记的核苷酸探针,利用荧光原位杂交技术(FISH)对132例疑似尿路感染患者中段尿标本进行检测;同时进行中段尿培养.结果 荧光原位杂交法检测阳性的有9例,与中段尿培养比较,其敏感度为100.0%,特异度为99.2%,阳性预期值为90.0%,阴性预期值为100.0%.结论 荧光原位杂交能快速检测尿液中的金黄色葡萄球菌,对金黄色葡萄球菌所致的尿路感染进行快速诊断.%Objective The rapid detection of Staphylococcus aureus in urine by fluorescence in situ hybridization (FISH)method. Screening urinary tract infection (UTIs) caused by Staphylococcus aureus. Methods Probes that were specific for Staphylococcus aureus were designed based on the conserved 16 s RNA sequences. Detected a total of 132 urine samples using FISH method. And all of tbese samples tested via traditional culturing techniques. Results 9 of these samples tested positive for a UTI via FISH analysis. Compared to conventional methods of bacterial identification,the FISH method had a sensitivity of 100.0% ,specificity of 99. 2%,positive expected value of 90. 0% and negative expected value of 100. 0%. Conclusion FISH had the potential to become an extremely useful diagnostic tool for UTIs because it has a quick turnaround time and high accuracy.

  18. D(E)TECTION DE LA MUTATION DU G(E)NE HMLH1 DANS LE CANCER COLO-RECTAL PAR(E)LECTROPHOR(E)SE CAPILAILRE ASSOCI(E)E (A) LA FLUORESCENCE LASER-INDUITE

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objectif L'objectif de ce travail est d'etablir une méthode d'analyse du polymorphisme de la conformation monochaine ( PCMC), capable de détecter la mutation ponctuelle du gène hMLH1 dans le cancer colique en utilisant l' l ctrophor se capillaire associée à la fluorescence laser-induite (FLI-EC). Méthodes Les exons 12 du gène hMLH1 du sang périphérique de 42 patients de cancer colo-rectal sporadique et 20 sujets sains témoins sont amplifiés par la PCR. Le PCMC des produits du PCR est analysé par FLI-EC. Les échantillons anormaux ont été confirmés par séquen age EC. Les effets de la concentration du milieu ( LPA ) , de la température de séparationcet du voltage de séparation sur le comportement de EC ont été aussi etudiés. Résultats L' analyse de PCMC par la methode FLI-EC a retrouvé une mutation hétérozygote chez 4 des 42 patients. La mutation ponctuelle de T1l51A a été objectivée par la séquen age EC de ces échantillons, aucune mutation n'a été retrouvée chez les 20 sujets sains témoins. La séparation des pics d'ADN monochainaux a été facilitée par une légère augmentation de la concentration de LPA (4%-6% ), une légère baisse de la température de séparation ( 20℃) et une légère élévation du voltage de séparation ( 9kV). Conclusion Une concentration adéquate de LPA, une température de séparation appropriée et un voltage de séparation bien approprié améliorent considérablement l'efficacité du FLI-EC.L'application de cette méthode pour détecter la mutation ponctuelle du gène hMLH1 est d'une rapidité, d'une efficacité, et d'uneéreproductivit consid rables. Cette méthode de réalisation facile reste très prometteur pour le dépistage rapide et massive des mutations génétiques.

  19. Fluorescence in situ hybridization(FISH) technique combined with karyotyping analysis in prenatal diagnosis%FISH技术在产前诊断中的应用价值研究

    Institute of Scientific and Technical Information of China (English)

    沈国松; 张甦; 何平亚; 方嵘

    2011-01-01

    目的 使用荧光原位杂交(FISH)技术对常见胎儿染色体数目异常进行快速诊断,结合羊水细胞培养染色体核型分析技术形成产前诊断体系,并对其临床应用价值进行评价.方法 应用诊断最常见染色体病的5种染色体(13、18、21、X和Y)特异性FISH探针对480例未经培养羊水细胞进行产前诊断,并和同时进行的羊水培养染色体核型分析相比较.结果 480例未经培养羊水细胞FISH实验全部获得检测结果,并发现21-三体综合征2例,18-三体综合征1例,克氏综合征1例,特纳综合征 1例,与羊水培养染色体分析结果一致,但报告时间(2至3d)与羊水染色体分析报告时间(2~3周)相比大为缩短.受固有技术限制有6例结构异常未能检出,但应用FISH技术对其中1例与性染色体有关的结构异常进行辅助诊断,获得了成功.结论 FISH技术在常见染色体数目异常产前诊断中应用行之有效,与羊水染色体核型分析技术相结合,将使产前诊断更加高效和安全.%To evaluate the clinical application of the combination of fluorescence in situ hybridization (FISH)technique and karyotyping analysis in prenatal diagnosis.MethodsFISH technology with the centromeric probes of chromo-some 13, 18, 21 ,X and Y was applied in 480 amniotic fluid specimens, the results were compared with those of the G banding karyotypes from standard cytogenetic analysis in cultured amniotic fluid cells.ResultsTotal 480 uncultured amniotic fluid speci-mens were successfully tested by FISH and two Down' s syndrome, one Edwards syndrome, one Klinefelter syndrome and one Turner syndrome were detected. The results obtained by FISH were consistent with those from karyotyping analysis. FISH (2-3 days) was faster than karyotyping(2-3 weeks) to get results. Karyotypes analysis detected 6 cases of chromosome structural abnormalities which were not detected by FISH, however, one of them with a sex chromosome-related structural

  20. Subtelomeric rearrangements in Indian children with idiopathic intellectual disability/developmental delay: Frequency estimation & clinical correlation using fluorescence in situ hybridization (FISH)

    Science.gov (United States)

    Mohan, Shruthi; Koshy, Teena; Vekatachalam, Perumal; Nampoothiri, Sheela; Yesodharan, Dhanya; Gowrishankar, Kalpana; Kumar, Jeevan; Ravichandran, Latha; Joseph, Santhosh; Chandrasekaran, Anupama; Paul, Solomon F. D.

    2016-01-01

    Background & objectives: Subtelomeres are prone to deleterious rearrangements owing to their proximity to unique sequences on the one end and telomeric repetitive sequences, which increase their tendency to recombine, on the other end. These subtelomeric rearrangements resulting in segmental aneusomy are reported to contribute to the aetiology of idiopathic intellectual disability/developmental delay (ID/DD). We undertook this study to estimate the frequency of subtelomeric rearrangements in children with ID/DD. Methods: One hundred and twenty seven children with idiopathic ID/DD were tested for subtelomeric rearrangements using karyotyping and FISH. Blood samples were cultured, harvested, fixed and GTG-banded using the standard protocols. Results: Rearrangements involving the subtelomeres were observed in 7.8 per cent of the tested samples. Detection of rearrangements visible at the resolution of the karyotype constituted 2.3 per cent, while those rearrangements detected only with FISH constituted 5.5 per cent. Five deletions and five unbalanced translocations were detected. Analysis of parental samples wherever possible was informative regarding the inheritance of the rearrangement. Interpretation & conclusions: The frequency of subtelomeric rearrangements observed in this study was within the reported range of 0-35 per cent. All abnormal genotypes were clinically correlated. Further analysis with array technologies presents a future prospect. Our results suggest the need to test individuals with ID/DD for subtelomeric rearrangements using sensitive methods such as FISH. PMID:27934799

  1. Subtelomeric rearrangements in Indian children with idiopathic intellectual disability/developmental delay: Frequency estimation & clinical correlation using fluorescence in situ hybridization (FISH

    Directory of Open Access Journals (Sweden)

    Shruthi Mohan

    2016-01-01

    Full Text Available Background & objectives: Subtelomeres are prone to deleterious rearrangements owing to their proximity to unique sequences on the one end and telomeric repetitive sequences, which increase their tendency to recombine, on the other end. These subtelomeric rearrangements resulting in segmental aneusomy are reported to contribute to the aetiology of idiopathic intellectual disability/developmental delay (ID/DD. We undertook this study to estimate the frequency of subtelomeric rearrangements in children with ID/DD. Methods: One hundred and twenty seven children with idiopathic ID/DD were tested for subtelomeric rearrangements using karyotyping and FISH. Blood samples were cultured, harvested, fixed and GTG-banded using the standard protocols. Results: Rearrangements involving the subtelomeres were observed in 7.8 per cent of the tested samples. Detection of rearrangements visible at the resolution of the karyotype constituted 2.3 per cent, while those rearrangements detected only with FISH constituted 5.5 per cent. Five deletions and five unbalanced translocations were detected. Analysis of parental samples wherever possible was informative regarding the inheritance of the rearrangement. Interpretation & conclusions: The frequency of subtelomeric rearrangements observed in this study was within the reported range of 0-35 per cent. All abnormal genotypes were clinically correlated. Further analysis with array technologies presents a future prospect. Our results suggest the need to test individuals with ID/DD for subtelomeric rearrangements using sensitive methods such as FISH.

  2. Detection of MDM2/CDK4 amplification in lipomatous soft tissue tumors from formalin-fixed, paraffin-embedded tissue: comparison of multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Creytens, David; van Gorp, Joost; Ferdinande, Liesbeth; Speel, Ernst-Jan; Libbrecht, Louis

    2015-02-01

    In this study, the detection of MDM2 and CDK4 amplification was evaluated in lipomatous soft tissue tumors using multiplex ligation-dependent probe amplification (MLPA), a PCR-based technique, in comparison with fluorescence in situ hybridization (FISH). These 2 techniques were evaluated in a series of 77 formalin-fixed, paraffin-embedded lipomatous tumors (27 benign adipose tumors, 28 atypical lipomatous tumors/well-differentiated liposarcomas, 18 dedifferentiated liposarcomas, and 4 pleomorphic liposarcomas). Using MLPA, with a cut-off ratio of >2, 36/71 samples (22 atypical lipomatous tumors/well-differentiated liposarcomas, and 14 dedifferentiated liposarcomas) showed MDM2 and CDK4 amplification. Using FISH as gold standard, MLPA showed a sensitivity of 90% (36/40) and a specificity of 100% (31/31) in detecting amplification of MDM2 and CDK4 in lipomatous soft tissue tumors. In case of high-level amplification (MDM2-CDK4/CEP12 ratio >5), concordance was 100%. Four cases of atypical lipomatous tumor/well-differentiated liposarcoma (4/26, 15%) with a low MDM2 and CDK4 amplification level (MDM2-CDK4/CEP12 ratio ranging between 2 and 2.5) detected by FISH showed no amplification by MLPA, although gain of MDM2 and CDK4 (ratios ranging between 1.6 and 1.9) was seen with MLPA. No amplification was detected in benign lipomatous tumors and pleomorphic liposarcomas. Furthermore, there was a very high concordance between the ratios obtained by FISH and MLPA. In conclusion, MLPA proves to be an appropriate and straightforward technique for screening MDM2/CDK4 amplification in lipomatous tumors, especially when a correct cut-off value and reference samples are chosen, and could be considered a good alternative to FISH to determine MDM2 and CDK4 amplification in liposarcomas. Moreover, because MLPA, as a multiplex technique, allows simultaneous detection of multiple chromosomal changes of interest, it could be in the future a very reliable and fast molecular analysis on

  3. Fish Allergy

    Science.gov (United States)

    ... Old Feeding Your 1- to 2-Year-Old Fish Allergy KidsHealth > For Parents > Fish Allergy Print A ... From Home en español Alergia al pescado About Fish Allergy A fish allergy is not exactly the ...

  4. Rapid discrimination of Haemophilus influenzae, H. parainfluenzae, and H. haemolyticus by fluorescence in situ hybridization (FISH and two matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS platforms.

    Directory of Open Access Journals (Sweden)

    Hagen Frickmann

    Full Text Available BACKGROUND: Due to considerable differences in pathogenicity, Haemophilus influenzae, H. parainfluenzae and H. haemolyticus have to be reliably discriminated in routine diagnostics. Retrospective analyses suggest frequent misidentifications of commensal H. haemolyticus as H. influenzae. In a multi-center approach, we assessed the suitability of fluorescence in situ hybridization (FISH and matrix-assisted laser-desorption-ionization time-of-flight mass-spectrometry (MALDI-TOF-MS for the identification of H. influenzae, H. parainfluenzae and H. haemolyticus to species level. METHODOLOGY: A strain collection of 84 Haemophilus spp. comprising 50 H. influenzae, 25 H. parainfluenzae, 7 H. haemolyticus, and 2 H. parahaemolyticus including 77 clinical isolates was analyzed by FISH with newly designed DNA probes, and two different MALDI-TOF-MS systems (Bruker, Shimadzu with and without prior formic acid extraction. PRINCIPAL FINDINGS: Among the 84 Haemophilus strains analyzed, FISH led to 71 correct results (85%, 13 uninterpretable results (15%, and no misidentifications. Shimadzu MALDI-TOF-MS resulted in 59 correct identifications (70%, 19 uninterpretable results (23%, and 6 misidentifications (7%, using colony material applied directly. Bruker MALDI-TOF-MS with prior formic acid extraction led to 74 correct results (88%, 4 uninterpretable results (5% and 6 misidentifications (7%. The Bruker MALDI-TOF-MS misidentifications could be resolved by the addition of a suitable H. haemolyticus reference spectrum to the system's database. In conclusion, no analyzed diagnostic procedure was free of errors. Diagnostic results have to be interpreted carefully and alternative tests should be applied in case of ambiguous test results on isolates from seriously ill patients.

  5. Detection of denitrification genes by in situ rolling circle amplification - fluorescence in situ hybridization (in situ RCA-FISH) to link metabolic potential with identity inside bacterial cells

    DEFF Research Database (Denmark)

    Hoshino, Tatsuhiko; Schramm, Andreas

    2010-01-01

    A target-primed in situ rolling circle amplification (in situ RCA) protocol was developed for detection of single-copy genes inside bacterial cells and optimized with Pseudomonas stutzeri, targeting nitrite and nitrous oxide reductase genes (nirS and nosZ). Two padlock probes were designed per gene......). Nevertheless, multiple genes (nirS and nosZ; nirS and the 16S rRNA gene) could be detected simultaneously in P. stutzeri. Environmental application of in situ RCA-FISH was demonstrated on activated sludge by the differential detection of two types of nirS-defined denitrifiers; one of them was identified...

  6. Fish Proteins as Targets of Ferrous-Catalyzed Oxidation: Identification of Protein Carbonyls by Fluorescent Labeling on Two-Dimensional Gels and MALDI-TOF/TOF Mass Spectrometry

    DEFF Research Database (Denmark)

    Pazos, Manuel; da Rocha, Angela Pereira; Roepstorff, Peter

    2011-01-01

    Protein oxidation in fish meat is considered to affect negatively the muscle texture. An important source of free radicals taking part in this process is Fenton's reaction dependent on ferrous ions present in the tissue. The aim of this study was to investigate the susceptibility of cod muscle...... dehydrogenase, creatine kinase, and enolase were the sarcoplasmic proteins most vulnerable to ferrous-catalyzed oxidation. Moreover, NDK, phosphoglycerate mutase, and GAPDH were identified in several spots differing by their pI, and those forms showed different susceptibilities to metal-catalyzed oxidation...

  7. Analisis Kebijakan dan Efektivitas Organisasi

    Directory of Open Access Journals (Sweden)

    Amir Syarifudin Kiwang

    2015-05-01

    Full Text Available Penerapan Peraturan Gubernur Nusa Tenggara Timur Nomor 8 Tahun 2011 tentang Organisasi dan Tata Kerja Unit Pelaksana Teknis Pendidikan dan Pelatihan Koperasi dan Usaha Mikro Kecil dan Menengah pada Dinas Koperasi dan Usaha Mikro Kecil dan Menengah Provinsi Nusa Tenggara Timur adalah dalam rangka untuk meningkatkan efektivitas kerja organisasi pada UPT Diklat Koperasi dan UMKM. Penelitian ini berusaha untuk melihat dampak kebijakan organisasi terhadap efektivitas organisasi dengan menggunakan enam elemen variabel dari kebijakan dan praktik manajemen. Keenam elemen tersebut adalah penetapan tujuan strategis, pencarian dan pemanfaatan sumber daya, lingkungan prestasi, proses komunikasi, kepemimpinan dan pengambilan keputusan, serta adaptasi dan inovasi organisasi. Penelitian kualitatif ini menggunakan pendekatan studi kasus. Teknik analisis data yang digunakan adalah analisis data model Miles dan Huberman, yang terdiri atas reduksi data, display/penyajian data, dan kesimpulan/verifikasi. Hasil penelitian menunjukkan bahwa efektivitas organisasi pada UPT Diklat koperasi dan UMKM belum berjalan secara baik, hal ini dapat dilihat dari beberapa hal antara lain, belum adanya tenaga spesialisasi pengelola diklat dan tenaga khusus (sarjana di bidang perkoperasian dan kondisi lingkungan kerja (lingkungan prestasi pada UPT Diklat koperasi dan UMKM yang belum efektif. Penempatan pegawai juga belum tepat, mutasi ke UPT Diklat Koperasi dan UMKM tidak memperhatikan latar belakang pendidikan dan spesialisasi/keahlian pegawai sehingga membutuhkan waktu dalam proses penyesuaian serta menurunkan jumlah pelaksanaan diklat dikarenakan keterbatasan alokasi dana.

  8. Keratoacanthoma Dan Perawatannya

    OpenAIRE

    Indah Heriyanti

    2008-01-01

    Keratoacanthoma adalah suatu tumor jinak yang berasal dari jaringan epitel dan biasanya di jumpai pada permukaan kulit yang umumnya terjadi pada bagian kulit yang sering terkena sinar matahari dimana tumor ini dapat sembuh dengan spontan. Lesi ini biasanya berawal sebagai makula yang kecil yang berwarna merah dan kemudian menjadi papula yang kokoh dan bersisik pada permukaanya. Papul ini dengan cepat membesar selama 2 sampai 8 minggu. Pada awalnya berbentuk bulat atau oval,kokoh menonjol ...

  9. DEPRESI DAN GANGGUAN TIDUR

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    Wayan Eko Radityo

    2012-12-01

    Full Text Available Depresi merupakan gangguan mood berupa kesedihan yang intens, berlangsung dalamwaktu lama, dan mengganggu kehidupan normal yang insidennya semakin meningkatseiring dengan meningkatnya tekanan hidup. Tahun 2020, depresi diperkirakanmenempati urutan kedua penyakit di dunia. Gejala-gejala depresi terdiri dari gangguanemosi, gangguan kognitif, keluhan somatik, gangguan psikomotor, dan gangguanvegetatif. Salah satu gejala depresi yang muncul adalah gangguan tidur yang bisaberupa insomnia, bangun secara tiba-tiba, dan hipersomnia. Hal ini disebabkan olehgangguan neurotransmiter dan regulasi hormon. Selain sebagai gejala depresi, gangguantidur juga bisa merupakan penyebab depresi. Beberapa penelitian memberikanhubungan gangguan tidur dapat meningkatkan risiko depresi di kemudian hari.

  10. The application of fluorescence in situ hybridization (FISH technique for studying the microbial communities in intestinal tissues of white shrimp (Penaeus vannamei

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available Fluorescence in situ hybridization technique is very useful for the evaluation of microbial communities in various environments. It is possible to apply this technique to study the intestinal microflora in white shrimp (Penaeus vannamei. Different fixatives and storage temperature were tested in this technique. It was found that fixation with 10% buffered formalin for 12 hours and changed to 70% ethanol shown positive results when compared to the fixation with Davidson's fixative or RF fixative. The best signaling was obtainedfrom the samples which were stored in -20ºC. By using the DNA probe targeted to the Eubacteria domain (EUB338 probe, 5′-GCT GCC TCC CGT AGG AGT-3′ labeled with fluorescein as a hybridizing probe, it was found that most intestinal microflora were aggregated with the intestinal contents, or dispersed in the lumen. There was not evidence of the attachment of the microflora with the intestinal epithelium in this study.

  11. Determination of methyl mercury in fish by toluene extraction and atomic fluorescence spectrometry%甲苯萃取-原子荧光光谱法检测鱼肉中甲基汞

    Institute of Scientific and Technical Information of China (English)

    史永富; 田良良; 黄冬梅; 蔡友琼; 黄宣运; 于慧娟

    2014-01-01

    Objective To establish a method for the determination of methyl mercury in fish by toluene extraction and atomic fluorescence spectrometry (AFS). Methods Samples were digested by HBr(48%, v/v) for 30 min, and methyl mercury was extracted by methylbenzene. The extract was back-extracted into aqueous solution by Na2S2O3. The aqueous solution was ready for the analysis by atomic fluorescence spectrometry (AFS). And the present method was used to determine the concentration of methyl mercury in reference materials. Results The result was in accordance with the assigned value. Recoveries were 96.1%and 97.5% respectively. Conclusion The method is effective to determine methyl mercury in fish for its high sensitivity and reproducibility.%目的:建立甲苯萃取-原子荧光光谱检测鱼肉中甲基汞的方法。方法样品经48%氢溴酸水解、甲苯萃取、0.05 mol/L硫代硫酸钠反萃取后,应用AFS-9230原子荧光光谱法进行测定,同时经此前处理后的样品采用DMA-80直接测汞仪进行测定,与原子荧光光谱法的结果进行对比。结果原子荧光光谱法以及直接测汞仪直接测定的结果与标准物质定值吻合,确证该方法的可靠性,适用于鱼、贝等水产品中甲基汞的测定。采用该方法对鱼肉中总汞与甲基汞成分分析标准物质 GBW10029以及英国食品分析水平评估计划(food analysis performance assessment scheme, FAPAS)的罐装鱼肉样品(样品编号07189)进行了检测,检测结果与参考物定值吻合,回收率分别为96.1%,97.5%。结论该方法操作简单,灵敏度高,重现性好,适用于鱼肉中甲基汞含量的定量检测。

  12. Fish Hearing.

    Science.gov (United States)

    Blaxter, J. H. S.

    1980-01-01

    Provides related information about hearing in fish, including the sensory stimulus of sound in the underwater environment, mechanoreceptors in fish, pressure perception and the swimbladder, specializations in sound conduction peculiar to certain fish families. Includes numerous figures. (CS)

  13. Fish Allergy

    Science.gov (United States)

    ... in a clear and consistent manner, so that consumers with food allergies and their caregivers can be informed as ... the menu, cross-contact with fish is possible. Ethnic ... fish. Avoid foods like fish sticks and anchovies. Some individuals with ...

  14. Chromosome painting by GISH and multi-color FISH

    Science.gov (United States)

    Fluorescent in situ hybridization (FISH) is a powerful cytogenetic technique for identifying chromosomes and mapping specific genes and DNA sequences on individual chromosomes. Genomic in situ hybridization (GISH) and multi-color FISH (mc-FISH) represent two special types of FISH techniques. Both ...

  15. APLIKASI TEKNIK AAN DI REAKTOR RSG-GAS PADA PENENTUAN UNSUR ESENSIAL DAN TOKSIK DI DALAM IKAN DAN PAKAN IKAN

    Directory of Open Access Journals (Sweden)

    Saeful Yusuf

    2015-03-01

    Full Text Available Pada makalah ini diuraikan tentang aplikasi teknik AAN (Analisis Aktivasi Neutron dalam penentuan konsentrasi unsur-unsur esensial dan cemaran yang terkandung di dalam beberapa spesies ikan dan pakan ikan. Unsur-unsur esensial yang terkandung dalam pakan ikan buatan juga dianalisis untuk mengetahui pengaruhnya terhadap ikan. Penentuan unsur menggunakan teknik AAN dengan metode perbandingan dan metode k0-AAN. Sampel diiradiasi di reaktor RSG-GAS yang memiliki fluks neutron thermal 5 x 1013 n.cm-2.s-1 pada daya 15 MW. Hasil penelitian menunjukkan bahwa 12 unsur di dalam 11 spesies ikan air laut dan air tawar telah ditentukan yaitu As, Br, Cr, Co, Cs, Fe, Hg, K, Na, Rb, Se and Zn. Konsentrasi cemaran As didalam ikan laut sudah melampaui batas maksimum 1 mg/kg, sedangkan konsentrasi cemaran Hg masih dibawah batas maksimum 0,5 mg/kg, baik untuk ikan laut maupun ikan air tawar. Unsur K dan Na merupakan unsur makroesensial sedangkan unsur Cr, Co, Fe, Se and Zn adalah termasuk unsur mikroesensial. Secara umum ditunjukkan bahwa kandungan mineral didalam ikan laut lebih tinggi konsentrasinya dibandingkan ikan air tawar. Br, Cs dan Rb merupakan unsur-unsur non esensial yang teridentifikasi dalam semua ikan yang dianalisis. Penelitian terhadap pakan ikan air tawar menunjukkan bahwa semua unsur yang teridentifikasi juga terdapat di dalam ikan laut dan ikan air tawar. Hal ini menunjukkan bahwa pakan ikan berkontribusi terhadap konsentrasi unsur di dalam ikan air tawar. Kata kunci : Analisis aktivasi neutron, unsur esensial, unsur cemaran, ikan, pakan ikan   This paper reported on the application of NAA (Neutron Activation Analysis Technique in the determination of the concentration of the essential and toxic elements in some species of fish and fish feed. Determination of elements using instrumental NAA technique with comparison and k0-INAA methods. Samples were irradiated in the RSG-GAS which has a thermal neutron flux  5.0E +13 ncm-2s-1. The results

  16. TAKTIK PENANGKAPAN TUNA MATA BESAR (Thunnus obesus DI SAMUDERA HINDIA BERDASARKAN DATA HOOK TIMER DAN MINILOGGER

    Directory of Open Access Journals (Sweden)

    Andi Bahtiar

    2016-04-01

    Full Text Available Penangkapan ikan tuna di Samudera Hindia semakin kompetitif, sehingga setiap Nahkoda atau Fishing Master kapal rawai tuna perlu memiliki taktik penangkapan ikan yang efektif dan efisien. Pengetahuan mengenai tingkah laku ikan tuna merupakan informasi penting untuk merumuskan taktik penangkapan ikan yang efektif dan efisien. Penelitian taktik penangkapan ikan tuna, khususnya tuna mata besar (Thunnus obesus di Samudera Hindia berdasarkan data hook timer dan minilogger dilakukan mulai Juni 2007 sampai Januari 2010, dengan metode observasi langsung pada kapal rawai tuna yang berbasis di Pelabuhan Benoa. Hasil penelitian menunjukkan bahwa tuna mata besar aktif mencari makan mulai pukul 13:00-18:00, dan lebih banyak tertangkap pada kedalaman 194-470 m dengan suhu air antara 8,4-15,50C. Oleh karena itu taktik penanagkapan yang tepat untuk diterapkan adalah pengaturan waktu setting pada siang hari dan hauling pada malam hari dan memadukan antara konstruksi rawai tuna bersifat pertengahan (halfway longline dan dalam (deep longline.   Tuna fishing in Indian Ocean more competitive, so Fishermans or Fishing Masters of tuna longliner must have an effective and efficient of fishing tactics. Knowledge on tuna behavior is an important information to formulate the fishing tactics. A study on bigeye tuna fishing tactics based on hook timer and minilogger data of tuna longliner was conducted in Indian Ocean from June 2007 to January 2010. Onboard observation of tuna longliner has been done at Benoa Port. The results showed that the feeding periodicity of bigeye tunas started from 1pm to 6pm, and mostly caught at water depth of 194 to 470 m with the water temperature between 8.4 to 15.2 degree celcius so the set times during the day and the haul times at night and the combination of halfway tuna longline and deep tuna longline construction are the best fishing tactics to be applied.

  17. STRUKTUR KOMUNITAS IKAN KARANG DI PERAIRAN PULAU RAYA, PULAU RUSA, PULAU RONDO DAN TAMAN LAUT RINOI DAN RUBIAH, NANGGROE ACEH DARUSSALAM

    Directory of Open Access Journals (Sweden)

    Isa Nagib Edrus

    2016-03-01

    Full Text Available Penelitian struktur komunitas ikan karang dilakukan di 10 stasiun pada perairan pulau terluar dan 2 stasiun pada taman laut Nanggroe Aceh Darussalam.  Tujuan penelitian untuk mengindentifikasi struktur komunitas ikan karang. Metode yang digunakan adalah transek sabuk dan sensus visual dalam area 250 m2. Hasil penelitian menunjukkan bahwa didapat 235 jenis ikan karang yang mewakili 45 suku. Komposisi jenis dan keanekaragaman (H bervariasi antar stasiun. Pulau Raya memiliki jumlah jenis ikan karang dan keanekaragaman jenis yang paling rendah dibanding Pulau Rusa dan Pulau Rondo. Lokasi Pulau Rondo lebih jauh dari daratan utama dan memiliki jenis dan keanekaragaman yang lebih tinggi dari pada Pulau Raya dan Rusa, tetapi semua itu masih rendah jika dibandingkan dengan dua lokasi taman laut, Rinoi dan Rubiah. Ikan hias yang umum dijumpai di perairan Nanggroe Aceh Darusasalam, tetapi jarang dijumpai di tempat lain, adalah dari jenis kepe-kepe seperti Chaetodon andamanensis, Chaetodon xanthocephalus, Hemitaurichthys zoster dan jenis ikan antias punggung kuning, Pseudanthias  evansi. Sementara, kepadatan individu per meter persegi tergolong rendah pada semua stasiun penelitian.   The community structure study of reef fish was conducted in 10 stations of the adjacent bordered off islands and 2 stations of  around sea gardens of Nanggroe Aceh Darussalam waters. The study objective is to identify the reef fish community structures. Methods used was belt transect and visual census within area of   250 m2. The results showed that there were at least 235 species of reef fishes with 45 families. Species compositions and diversity indices (R were varied among transect sites. Raya Island have the lowest of reef fish species numbers and it’s diversity than those in Rusa and Rondo islands. Rondo island, the remote area from the main land, have the higher  species numbers and diversity than those in Raya and Rusa islands; however, species numbers and

  18. ANALISIS SPASIAL DAN TEMPORAL AKTIVITAS KAPAL IKAN DI PERAIRAN SELATAN ARU MENGGUNAKAN CITRA MODIS DAN RADARSAT-2

    Directory of Open Access Journals (Sweden)

    Dendy Mahabror

    2016-11-01

    Full Text Available Perairan selatan Aru merupakan daerah penangkapan  ikan yang potensial dengan sumberdaya ikannya yang berlimpah. Kapal-kapal ikan melakukan penangkapan ikan dengan mempertimbangkan kondisi kesuburan perairan yaitu fenomena upwelling melalui parameter suhu permukaan laut dan kelimpahan konsentrasi klorofil-a. Penggunaan citra MODIS secara periodik dapat menggambarkan waktu dan lokasi upwelling. Variabilitas suhu permukaan laut dan konsentrasi klorofil-a di lintang 7 LS dan 7.5 LS, bujur 133.1 – 136 BT menunjukkan fenomena upwelling pada musim Timur dimana suhu permukaan laut menurun hingga 24 oC sedangkan konsentrasi klorofil-a rata-rata meningkat hingga 3.61 mg/m3. Fenomena downwelling terjadi di musim Barat dimana suhu permukaan laut di lintang yang sama menjadi hangat berkisar 30 oC dengan konsentrasi rata-rata klorofil-a <1 mg/m3. Jumlah kapal ikan yang beroperasi di daerah penangkapan diketahui dengan menggunakan citra Radarsat-2 dan data VMS yang disesuaikan dengan waktu pengambilan citra di setiap bulannya dengan lokasi yang sama. Analisis spasial dan temporal dilakukan untuk mengetahui sebaran kapal ikan pada musim timur saat kesuburan perairan tinggi dimana konsentrasi klorofil-a di titik penangkapan mencapai 5.01 mg/m3 dan jumlah kapal ikan yang beroperasi >150 unit/hari. Pada musim Barat kesuburan perairan rendah dimana konsentrasi klorofil-a di titik penangkapan <1 mg/m3 dan jumlah kapal ikan yang beroperasi <40 unit/hari. KATA KUNCIklorofil-a, Modis, perairan selatan Aru, Radarsat-2, suhu permukaan laut. SPATIAL AND TEMPORAL ANALYSIS OF WATER PRODUCTIVITY AFFECTING ACTIVITIES IN FISHING VESSEL AT SOUTH ARU FISHING GROUND USING MODIS IMAGE AND RADARSAT-2Southern Aru waters is a potential fishing areas with abundant fish resources. Fishing boats catch fish by considering the condition of the waters productivity such as upwelling phenomenon through the parameters of sea surface temperatures and an abundance of chlorophyll

  19. Dan Performer Mei Lanfang

    DEFF Research Database (Denmark)

    Risum, Janne

    2010-01-01

    The convention of performing female characters (dan characters) in Beijing opera, as practised by its most prominent male performer of female characters Mei Lanfang, and its and his cultural context and aesthetic aim...

  20. Liever kraanwater dan bronwater

    NARCIS (Netherlands)

    Kole, A.P.W.

    2011-01-01

    Een test in het Restaurant van de Toekomst van de invloed van CO2-labels op het aankoopgedrag van consumenten, heeft nog geen duidelijk beeld opgeleverd. Wel pakten mensen vaker kraanwater dan bronwater.

  1. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2016-01-01

    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  2. Fish allergy and fish allergens

    DEFF Research Database (Denmark)

    Kuehn, A; Hilger, Christiane; Ollert, Markus

    2016-01-01

    but patients with this phenotype constitute an important sub-group among fish-allergic individuals. 2. Newly identified fish allergens, enolases, aldolases, and fish gelatin, are of high relevance as the majority of the fish-allergic individuals seem to develop specific IgE against these proteins. The present...

  3. Makna Dan Ideologi Punk

    OpenAIRE

    Daniar Wikan Setyanto

    2015-01-01

    Punk Sebagai sebuah subkultur telah diadaptasi oleh para remaja Indonesia bukan hanya sebagai semangat bermusik, namun merambah sebagai ideologi dan gaya hidup. Punk merupakan warisan budaya dari barat yang awalnya bergerak pada bidang musik, namun lambat lain punk telah menjadi sebuah identitas global yang mewakili para remaja radikal yang memiliki visi anti kemapanan dan anti pemerintah. Selain ideologinya yang kontroversi, punk mempengaruhi dinamika fashion di dunia. Asesoris punk diadapta...

  4. STATUS PEMANFAATAN DAN PENGELOLAAN SUMBERDAYA IKAN TUNA NERITIK DI SAMUDERA HINDIA WPP 572 DAN 573

    Directory of Open Access Journals (Sweden)

    Agustinus Anung Widodo

    2014-05-01

    Full Text Available Dalam rangka mendeskripsikan status pemanfaatan dan pengelolaan sumberdaya tuna neritik di perairan Samudera Hindia (WPP 572 dan 573 telah dilakukan analisis terhadap informasi tentang jenis dan produksi tuna neritik yang disajikan dalam Statistik Perikanan Tangkap di Laut Menurut WPP tahun 2005-2012 (DJPT, 2013 serta data hasil penelitian berbasis di PPS Cilacap dan PPN Sibolga tahun 2011. Rekomendasi ‘working party’ tentang tuna neritik dari IOTC dikaji sebagai langkah pengelolaan perikanan tuna neritik di Indonesia. Hasil analisis dan kajian menunjukkan bahwa sumberdaya ikan tuna neritik yang tertangkap nelayan Indonesia di perairan WPP 572 dan 573 meliputi tongkol lisong (Auxis rochei, tongkol krai (Auxis thazard, tongkol komo atau kawakawa (Euthynnus affinis dan tongkol abu-abu (Thunnus tonggol. Tuna neritik tertangkap sebagai by-product dari pukat cincin, jaring insang hanyut, pancing tonda, pancing ulur dan bagan. Tahun 2011 produksi neritik tuna di Samudera Hindia khususnya WPP 572 dan 573 mencapai 121.818 ton atau 29,4% dari total produksi tuna neritik nasional. Tuna neritik jenis tongkol lisong dan krai yang tertangkap jaring insang hanyut yang berbasis di Cilacap > 70% merupakan ikan yang telah dewasa. Adapun tongkol komo yang tertangkap pukat cincin yang berbasis di Sibolga sekitar 55,5% merupakan ikan dewasa. Belum ada langkah-langkah pengelolaan secara spesifik terhadap sumberdaya tuna neritik di Indonesia. Merujuk hasil Working Party on Neritic Tuna pertama dan kedua tahun 2011 dan 2012, Indian Ocean Tuna Commission (IOTC merekomendasikan adanya kerjasama antar negara anggota IOTC yang saling berdekatan didalam melakukan pengelolaan sumberdaya neritik tuna. Langkah pertama adalah dilakukan perelitian mengenai populasi melalui studi mtDNA untuk memastikan status stok dan populasinya.   The species of neritic tuna caught by fishers in the Indian Ocean particularly FMAs 572 and 573 consisted of frigate tuna (Auxis thazard

  5. Recent Advances in Fluorescence in situ Hybridization

    OpenAIRE

    吉田, 廸弘; Michihiro C., Yoshida; 北海道大学理学部附属動物染色体研究施設; Chromosome Research Unit, Faculty of Science, Hokkaido University

    1992-01-01

    Fluorescence in situ hybridization (FISH) procedures that directly couple molecular and cytological information allow precise visualization of DNA sequences on metaphase chromosomes and interphase nuclei. These techniques can be used to identify chromosomes, detect chromosomal aberrations, and analyze linear and spetial genome organization. FISH procedures are also used to clinical fields for diagnosis of disease-related chromosome changes and tumor biology.

  6. Renal and urinary findings in 20 patients with Williams-Beuren syndrome diagnosed by fluorescence in situ hybridization (FISH Achados renais e urinários em 20 pacientes com síndrome de Williams-Beuren diagnosticados pelo teste de hibridização in situ por fluoresceína (FISH

    Directory of Open Access Journals (Sweden)

    Sofia Mizuho Miura Sugayama

    2004-01-01

    Full Text Available PURPOSE: Williams-Beuren syndrome is a rare multiple anomalies/mental retardation syndrome caused by deletion of contiguous genes at chromosome region 7q11.23. The aim of this work was to determine the frequency and the types of renal and urinary tract anomalies in 20 patients with Williams-Beuren syndrome. METHODS: The fluorescence in situ hybridization test using a LSI Williams syndrome region DNA probe was performed for all 20 patients to confirm the diagnosis of Williams-Beuren syndrome. A prospective study was performed in order to investigate renal and urinary aspects using laboratory assays to check renal function, ultrasonography of the kidneys and urinary tract, voiding cystourethrogram and urodynamics. RESULTS: Deletion of the elastin gene (positive fluorescence in situ hybridization test was found in 17 out of 20 patients. Renal alterations were diagnosed in 5 of 17 (29% the patients with the deletion and in 1 of 3 patients without the deletion. Fourteen patients with the deletion presented dysfunctional voiding. Arterial hypertension was diagnosed in 3 patients with deletions and 1 of these presented bilateral stenosis of the renal arteries. CONCLUSIONS: Due to the high incidence of renal and urinary abnormalities in Williams-Beuren syndrome, performing a systematic laboratory and sonographic evaluation of the patients is recommended.OBJETIVO: A síndrome de Williams-Beuren é uma rara síndrome de deleção de genes contíguos que cursa com múltiplas anomalias congênitas, deficiência mental e anomalias renais e urinárias. O objetivo deste trabalho foi determinar a freqüência e os tipos de anomalias renais e urinárias em 20 pacientes com síndrome de Williams-Beuren diagnosticados pelo teste de hibridização in situ por fluorescência. MÉTODOS: Estudou-se prospectivamente os aspectos renais e urinários através de avaliação laboratorial da função renal, ultrassonografia de rins e vias urinárias, uretrocistografia

  7. Antarctic Fishes.

    Science.gov (United States)

    Eastman, Joseph T.; DeVries, Arthur L.

    1986-01-01

    Explains the adaptations to Antarctic waters that Notothenioidei, a group of advanced bony fishes, have exhibited. Discusses the fishes' mechanisms of production of antifreeze properties and their capacities for neutral buoyancy in water. (ML)

  8. PERFORMA IKAN NILA (Oreochromis niloticus HASIL SEX REVERSAL, GENETICALLY MALE DAN YY PADA FASE PENDEDERAN PERTAMA

    Directory of Open Access Journals (Sweden)

    Odang Carman

    2016-11-01

    Full Text Available Penelitian ini dilakukan untuk mengkaji performa ikan nila hasil sex reversal (SRV, genetically male tilapia (GMT, dan YY pada fase pendederan pertama di akuarium. Benih ikan dipelihara selama 22 hari, dari umur 6 hari hingga 28 hari. Parameter yang diamati meliputi tingkat sintasan, persentase ikan jantan, laju pertumbuhan, dan biomassa. Hasil penelitian menunjukkan bahwa tingkat sintasan tidak berbeda (P>0,05 antar ketiga kelompok ikan dan kontrol (KN, berkisar antara 85,30%--86,20%. Persentase ikan jantan antara SRV (94,5% ± 1,32% vs. GMT (93,8% ± 1,25% dan GMT vs. YY (90,2% ± 1,83% tidak berbeda (P>0,05, sedangkan antara SRV lebih tinggi daripada YY (P0.05, ranged from 85.30%-86.20%. Percentage of male fish between SRV (94.5% ± 1.32% versus GMT (93.8% ± 1.25% and GMT versus YY (90.2% ± 1.83% were also similar (P>0.05, while SRV is higher than YY (P<0.05. Percentage of male fish in the three fish groups was higher than that of control (56.9% ± 3.62%. Growth of YY fish and GMT were higher compared to SRV and control fish (KN. The mean weight of YY fish at the end of the experiment reached 476 mg, GMT fish 447 mg, SRV fish 379 mg and control 342 mg. Biomass of YY, GMT and SRV fish were respectively higher by 41.3%, 32.9%, and 10.3% compared to control. With high performance and technical consideration in farm, GMT fish can be a potential alternative to be cultured in fish farm in order to increase aquaculture production of nile tilapia.

  9. ESTIMASI PARAMETER PERTUMBUHAN SERTA MORTALITAS IKAN TAWES DAN NILA DI DANAU TEMPE SULAWESI SELATAN

    Directory of Open Access Journals (Sweden)

    Samuel Samuel

    2016-03-01

    Full Text Available Penelitian tentang pertumbuhan, mortalitas dan laju eksploitasi terhadap ikan tawes dan nila di Danau Tempe dari bulan Februari sampai Nopember tahun 2010, bertujuan untuk mendapatkan informasi yang diperlukan dalam rangka pengelolaannya di Danau Tempe. Estimasi parameter pertumbuhan, mortalitas dan laju eksploitasi dihitung menggunakan program FISAT. Hasil Penelitian menunjukkan pertumbuhan ikan tawes dan nila di Danau Tempe termasuk rendah dibandingkan dengan pertumbuhan jenis ikan tawes dan nila di beberapa badan air lainnya di Indonesia. Parameter pertumbuhan Von Bertalanffy ikan tawes mempunyai panjang infinitif (L∞= 29,1 cm dengan laju pertumbuhan (K= 0,30/tahun dan laju eksploitasi (E= 0,46 sedikit dibawah nilai tangkap maksimum. Ikan nila mempunyai panjang infinitif  (L∞= 31,8 cm dengan laju pertumbuhan (K= 0,22/tahun dan laju eksploitasi (E sebesar 0,50, tepat pada nilai tangkap maksimum. Untuk pengelolaan dan pemanfaatan sumberdaya ikan di Danau Tempe agar tetap lestari perlu adanya pembatasan jumlah alat tangkap dan pengawasan terhadap aktivitas penangkapan di zona inti ketika air surut. Research on growth, mortality and exploitation rate of these two fish species in Lake Tempe was conducted from February to November 2010. The aim of the research was to obtain information for management of these two fish species in Lake Tempe. Estimation of growth, mortality and exploitation rate was calculated using FISAT program. The results showed that java barb had infinitive length (L∞ = 29.1 cm with a growth rate of 0.30/year and exploitation rate of 0.46, slightly below the optimum fishing value. Nile tilapia had infinitive length (L∞ = 31.8 cm with a growth rate of 0.22/year and exploitation rate of 0.50, same as the optimum fishing value. Growth rate of java barb and nile tilapia in Lake Tempe were lower compared with the growth of these two fish species in others water bodies in Indonesia. For management and utilization of fish

  10. Fish Dishes.

    Science.gov (United States)

    Derby, Marie

    2003-01-01

    Describes an art project that was inspired by Greek pottery, specifically dishes shaped as fish. Explains that fourth-grade students drew a fish shape that was later used to create their clay version of the fish. Discusses how the students examined the pottery to make decisions about color and design. (CMK)

  11. INOVASI DAN STRATEGI PENCAPAIANNYA

    Directory of Open Access Journals (Sweden)

    Liem Ferryanto

    2009-01-01

    Full Text Available Innovation is the way of life of any institution to profitably sustain its life. It starts with empathy, the ability to reach outside of ourselves and walk in someone else’s shoes, and optimal implementation of the newly advanced technology. Innovation shows its results through continuously hard working efforts known as "10 Thousand Hours Rule". As world uncertainty creates complexity we, instead of predicting, should therefore anticipate the future by creating and managing real options on contingent projects or elements of alternative optimal strategies. This should reflect into our portfolio strategy. Abstract in Bahasa Indonesia: Inovasi merupakan darah bagi suatu institusi untuk bisa hidup berkelanjutan serta menguntungkan. Inovasi berupa penemuan baru secara sistematis yang berawal dari empati, kemampuan untuk melihat dunia melalui mata orang lain, dan pemanfaatan secara optimal kemajuan teknologi yang ada. Inovasi baru menghasilkan buahnya melalui kerja keras, yaitu dengan mengikuti “Aturan 10 Ribu Jam” secara berkesinambungan. Ketidakpastian, interaksi, keterbatasan dan degradasi menciptakan kompleksitas tentang kebutuhan dan solusi di masa depan. Oleh sebab itu daripada meramalkan risiko yang bakal terjadi, kita sebaiknya memasang strategi berupa skenario untuk mereduksi akibat dari risiko masa depan yang tidak kita mengerti. Skenario ini dapat diperoleh lewat penciptaan dan penanganan beberapa pilihan nyata atas semua proyek antisipatif yang ada. Kata kunci: Inovasi, ketidakpastian dan kompleksitas, aturan 10 ribu jam, paradoks strategi, peta jalan, empati, kerja berkesinambungan.

  12. PENGELOLAAN PENELITIAN DAN PENGEMBANGAN KESEHATAN DI THAILAND, MYANMAR, DAN INDIA

    Directory of Open Access Journals (Sweden)

    Anorital, SKM Anorital, SKM

    2012-09-01

    Full Text Available Dalam bulan Januari 1991 yang lalu, Sdr. Anorital, SKM (Ka. Subbag. Pengumpulan dan PengolahanData Badan Litbangkes dan H. Syafwani Mirin, SKM (Ka. Bag. Keuangan Badan Litbangkes memperoleh fellowship dari WHO untuk melakukan studi perbandingan ke institusi-institusi penelitian kesehatan di Thailand,Myanmar, dan India.Berikut di bawah ini tulisan bersangkutan yang menggambarkan secara garis besar pengelolaan penelitian dan pengembangan kesehatan pada masing-masing negara obyek studi. Semoga informasi yang terkandung pada tulisan ini dapat bermanfaat bagi pengembangan Badan Penelitian dan Pengembangan Kesehatan.

  13. TINJAUAN KRIMINOLOGIS TERHADAP ILLEGAL FISHING YANG TERJADI DI KOTA MAKASSAR (Studi Kasus Tahun 2010-2013)

    OpenAIRE

    PRATOMO R.S, WILIATER

    2014-01-01

    2014 Wiliater Pratomo R.S (B111 09133), Tinjauan Kriminologis terhadap Illegal Fishing yang Terjadi di Kota Makassar (Studi Kasus Tahun 2010-2013) dibimbing oleh Andi Sofyan sebagai Pembimbing I, dan Amir Ilyas sebagai Pembimbing II. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang menjadi pendorong terjadinya tindak pidana Illegal Fishing dan untuk mengetahui upaya yang dilakukan Direktorat Polair Polda Sulselbar dala...

  14. DanRIS

    DEFF Research Database (Denmark)

    2009-01-01

    Windows 2000/XP/Vista platform computerprogram til DanRIS indberetning baseret på indskrivning af klienter på CPR, klientsforløb, faseforløb, ASI EuropASI composite score beregninger. Udgave: 2.1.0.5 Udgivelsesdato: 01-01-2009......Windows 2000/XP/Vista platform computerprogram til DanRIS indberetning baseret på indskrivning af klienter på CPR, klientsforløb, faseforløb, ASI EuropASI composite score beregninger. Udgave: 2.1.0.5 Udgivelsesdato: 01-01-2009...

  15. Makna Dan Ideologi Punk

    Directory of Open Access Journals (Sweden)

    Daniar Wikan Setyanto

    2015-08-01

    Full Text Available Punk Sebagai sebuah subkultur telah diadaptasi oleh para remaja Indonesia bukan hanya sebagai semangat bermusik, namun merambah sebagai ideologi dan gaya hidup. Punk merupakan warisan budaya dari barat yang awalnya bergerak pada bidang musik, namun lambat lain punk telah menjadi sebuah identitas global yang mewakili para remaja radikal yang memiliki visi anti kemapanan dan anti pemerintah. Selain ideologinya yang kontroversi, punk mempengaruhi dinamika fashion di dunia. Asesoris punk diadaptasi oleh budaya bopuler sebagai pernak-pernik fashion. Kini punk, telah menjadi bagian dari dunia fashion di seluruh dunia. Apa sebenarnya yang melatar belakangi munculnya Punk? bagaimana Punk bisa berkembang di Indonesia? Kata Kunci: punk, fashion, budaya populer

  16. MISKONSEPSI KONSEP ARCHAEBACTERIA DAN EUBACTERIA

    Directory of Open Access Journals (Sweden)

    Fina Nurul Khotimah

    2015-03-01

    Full Text Available Penelitian ini bertujuan untuk mengidentifikasi miskonsepsi siswa pada konsep Archaebacteria dan Eubacteria. Penelitian ini menggunakan metode survei dengan instrumen berupa pilihan ganda beralasan terbuka sebagai alat ukur. Unit analisis dalam penelitian adalah siswa MAN kelas X berjumlah 72 orang. Hasil analisis ditemukan ketidakpahaman pada konsep Archaebacteria dan Eubacteria (61% yang mendominasi kategori lainnya. Sedangkan kategori Paham konsep dan Miskonsepsi hanya sebesar 19% dan 20%. Miskonsepsi yang dialami siswa dari hasil diagnosa alasan terbuka menunjukkan lebih banyak miskonsepsi utuh dibandingkan dengan miskonsepsi sebagian. Miskonsepsi utuh teridentifikasi dengan siswa beranggapan bahwa bakteri lebih banyak menimbulkan kerugian daripada keuntungan bagi manusia dan lingkungan.

  17. Precise measurement of the absolute fluorescence yield of nitrogen in air. Consequences on the detection of ultra-high energy cosmic rays; Mesure precise du rendement absolu de la fluorescence de l'azote dans l'air. Consequences sur la detection des rayons cosmiques d'ultra-haute energie

    Energy Technology Data Exchange (ETDEWEB)

    Lefeuvre, G

    2006-07-15

    The study of the energy spectrum of ultra-high energy cosmic rays (E > 10{sup 20} eV) requires to determine the energy with much more precision than what is currently achieved. The shower of particles created in the atmosphere can be detected either by sampling particle on the ground, or by detecting the fluorescence induced by the excitation of nitrogen by shower electrons. At present, the measurement of the fluorescence is the simplest and the most reliable method, since it does not call upon hadronic physics laws at extreme energies, a field still inaccessible to accelerators. The precise knowledge of the conversion factor between deposited energy and the number of fluorescence photons produced (the yield) is thus essential. Up to now, it has been determined with an accuracy of 15 % only. This main goal of this work is to measure this yield to better than 5 per cent. To do this, 1 MeV electrons from a radioactive source excite nitrogen of the air. The accuracy has been reached thanks to the implementation of a new method for the absolute calibration of the photomultipliers detecting the photons, to better than 2 per cent. The fluorescence yield, measured and normalized to 0.85 MeV, 760 mmHg and 15 Celsius degrees, is (4.23 {+-} 0.20) photons per meter, or (20.46 {+-} 0.98) photons per deposited MeV. In addition, and for the first time, the absolute fluorescence spectrum of nitrogen excited by a source has been measured with an optical grating spectrometer. (author)

  18. PERKEMBANGAN EMBRIO DAN LARVA IKAN LETTER SIX, Paracanthurus hepatus

    Directory of Open Access Journals (Sweden)

    Daniar Kusumawati

    2015-06-01

    Full Text Available Dalam upaya memperkaya populasi ikan letter six (Paracanthurus hepatus di alam yang telah mendekati kepunahan akibat adanya illegal fishing maka dilakukan usaha perbenihan. Kegiatan perbenihan ikan letter six memerlukan informasi awal tentang perkembangan telur dan pemeliharaan larva yang nantinya akan digunakan sebagai acuan dasar dalam menentukan pengembangan teknik pemeliharaan larva ikan letter six yang tepat. Koleksi induk ikan letter six hasil tangkapan di alam dari perairan Maluku dan Papua. Induk letter six dipelihara dalam tangki policarbonat bulat berkapasitas dua ton dengan sistem air mengalir. Selama pemeliharaan, induk letter six mampu memijah secara alami. Durasi perkembangan embrio hingga menetas memerlukan waktu 22 jam 24 menit pada suhu normal air laut 29°C. Penyerapan kuning telur optimum terjadi pada hari kedua dan maksimum terserap habis pada hari ketiga, sedangkan butir minyak terserap habis tepat pada hari ketiga. Korelasi pertumbuhan panjang dan tinggi larva mengikuti hubungan alometrik negatif di mana konstanta pertumbuhan b = 0,6629 (b < 1. Berdasarkan perkembangan larva, umur D-2 (terbentuk bintik mata mulai diberikan pakan alami berupa plankton dan pada D-3 (bukaan mulut mulai diberikan zooplankton yaitu rotifer. Dengan metode pemeliharaan ini, larva ikan letter six mati pada D-25 sehingga perlu adanya perbaikan metode pemeliharaan melalui pendekatan lingkungan maupun pakan.

  19. Macapat dan Santiswara

    OpenAIRE

    Darusuprapta, Darusuprapta

    2013-01-01

    Dalam khazanah kebudayaan Indonesia, khusus Jawa, hidup berkembangan kesenian macapat yang disebut juga  macapatan, dan kesenian santiswara yang disebut pula santiswaran. Macapatan lebih mengutamakan pembacaan teks dengan dendangan tembang kemudian diikuti sarasehan atau diskusi yang membahas segala segi isi teks bacaannya, sedangkan santiswaran lebih mengutamakan alunan swara dengan dendangan tembang disertai iringan bunyi tabuh-tabuhan.

  20. DANS LE CONTEXTE IVOIRIEN

    African Journals Online (AJOL)

    LA RELATION "GENRE" ET SES IMPLICATIONS SOCIOLOGIQUES. DANS LE CONTEXTE IVOIRIEN ... de base et de la gestion des affaires publiques en milieu rural .... Les raisons économiques; du fait du faible pouvoir d'achat des parents face au ..... cause de rupture du contrat, toute femme a le droit de suspendre son.

  1. Akromegali Dan Gigantisme

    OpenAIRE

    Silvani, Melati; Pase, M. Aron; Syafril, Santi; Lindarto, Dharma; Solin, Steffie S

    2016-01-01

    Hormon pertumbuhan manusia (Growth Hormone/GH) merupakan peptida rantai tunggal yang terdiri dari 191 asam amino, yang diisolasi dari sel somatotrop pada kelenjar hipofisis anterior pada tahun 1956, dan pertama kali digunakan sebagai terapi untuk penatalaksanaan dwarfisme hipofisis pada tahun 1958. Dwarfisme hipofisis adalah bentuk klasik dari defisiensi hormon pertumbuhan pada anak-anak Melati Silvani

  2. Comprehensive immunohistochemical analysis of Her-2/neu oncoprotein overexpression in breast cancer: HercepTest (Dako) for manual testing and Her-2/neuTest 4B5 (Ventana) for Ventana BenchMark automatic staining system with correlation to results of fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Mayr, Doris; Heim, Sibylle; Werhan, Cedric; Zeindl-Eberhart, Evelyn; Kirchner, Thomas

    2009-03-01

    Overexpression of Her-2/neu-oncoprotein is used as marker for Herceptin therapy. To investigate the sensitivity and specificity of automatic immunohistochemistry (Benchmark, Ventana), we compared the results to the manual testing (Dako) in 130 breast carcinomas and validated the results by fluorescence in situ hybridization (FISH). Manual and automatic immunohistochemistry of Her-2/neu-oncoprotein using two different antibodies (HercepTest, Her-2/neuTest 4B5) was analyzed. FISH was performed in all cases with uncertain or strong overexpression in either immunohistochemical stainings or with different immunohistochemical results. Same immunohistochemical results were seen in 73.8%. Two cases with overexpression, detected with Her-2/neuTest 4B5 and confirmed by FISH, showed no overexpression using HercepTest. From 21 cases with 2+ by Her-2/neuTest 4B5, 15 cases had no gene amplification (two of them with 3+ HercepTest); three cases showed a gene amplification (one of them with failing overexpression by HercepTest); two other cases were polysomic; one could not be analyzed. Ventana immunohistochemistry seems to be of same reliability like Dako with a little better concordance to FISH in our study.

  3. TINGKAT EFISIENSI PRODUKSI DAN PENDAPATAN PADA USAHA PENGOLAHAN IKAN ASIN SKALA KECIL

    Directory of Open Access Journals (Sweden)

    Himawan Arif Sutanto

    2015-08-01

    Full Text Available The aim of this research was to measure the level of production efficiency and calculate the return and cost salted fish processing small scale in Pekalongan municipality. There are 20 business owners of small scale processing of salted fish were sampled with snowball sampling. Stochastic Frontier Analysis is used to measure the level of efficiency and descriptive statistics are used to calculate the ratio of return and cost. The results indicate that the level of efficiency of production of salted fish processing small scale in Pekalongan yet efficient so it is still possible to be improved. Factors that influence the production of salted fish in Pekalongan is availability of fish, labor, equipment or facilities, auxiliary materials and extensive effort. Salted fish processing businesses in Pekalongan small scale is still quite favorable, as indicated by the R/C value of 1.37 which indicates that the amount of revenue entrepreneurs of small-scale fish processing is still greater than the costs to be incurred in running the business.Penelitian ini bertujuan untuk mengukur tingkat efisiensi produksi dan menghitung rasio biaya dan pendapatan usaha pengolahan ikan asin skala kecil di kota pekalongan. Sampel dalam penelitian ini adalaah 20 pemilik usaha ikan asin dengan metode snowball sampling. Analisis frontier stokastik digunakan untuk mengukur tingkat efisiensi dan metode deskriptif statistik digunakan untuk menghitung rasio biaya dan pendapatan. Hasil penelitian ini menunjukan bahwa usaha ikan asin skala kecil di kota pekalongan telah cukup efisien sehingga masih memungkinkan untuk ditingkatkan.  Faktor yang mempengaruhi efisiensi usaha ikan asin di kota pekalongan adalah ketersediaan bahan baku ikan, tenaga kerja, peralatan usaha, bahan penolong, dan luas usaha. Usaha pengolahan ikan asin di pekalongan masih menguntungkan, hal ini dapat terlihat dari nilau R/C sebesar 1,37 yang mengindikasikan keuntungan usaha masih lebih tinggi dibandingkan

  4. STATUS TERKINI DAN ALTERNATIF PENGELOLAAN SUMBER DAYA IKAN DI LAGUNA SEGARAANAKAN, CILACAP

    Directory of Open Access Journals (Sweden)

    Didik Wahyu Hendro Tjahjo

    2013-05-01

    Full Text Available Laguna Segara Anakan mempunyai peran penting sebagai kawasan pengelolaan perikanan udang sebagai daerah asuhan. Penyempitan dan pendangkalan perairan laguna sangat mempengaruhi populasi larva dan juvenil udang. Tujuan penulisan ini adalah untuk mengkaji hubungan perubahan lingkungan terhadap sumberdaya udang. Kandungan oksigen terlarut relatif rendah, kondisi tersebut dalam jangka panjang akan berpengaruh terhadap laju pertumbuhan ikan dan udang, dan akhirnya berdampak langsung terhadap laju rekruitmen udang dan ikan untuk perairan Segara Anakan dan perairan laut sekitar Cilacap. Kelimpahan larva udang dan ikan di perairan laguna ini berkisar antara 87 – 63,451 ind./1000 m3, dan kelimpahan juvenil tersebut berkisar antara 0 – 25,263 ind./1000 m2, dimana selama pengamatan didominasi oleh juvenil udang. Total produksi (ikan dan udang di Segara Anakan dari tahun ke tahun mengalami penurunan yang signifikan dalam periode tahun 1987-2004. Kondisi tersebut disebabkan luas perairan laguna semakin sempit dan dangkal, serta intensitas penangkapan semakin tinggi. Oleh karena itu, perlu suatu pengelolaan yang menyeluruh terhadap sumber daya laguna agar mampu mengurangi laju penurunan produksinya, yaitu pengelolaan sumberdaya perikanan di laguna Segara Anakan sebagai satu kesatuan ekologi yang utuh. Segara Anakan lagoon have an important role as the shrimp fishery management area in which shrimp species using this area of lagoons as a nursery ground. Refinement and siltation of the lagoon waters greatly affect populations of larvae and juvenile shrimp. The purpose of this paper is to study the relationship of environmental change on shrimp resources. Dissolved oxygen is relatively low, the condition will affect the growth of fish and shrimp in the long time, and ultimately it have a direct impact on the rate of recruitment of shrimp and fish for Segara Anakan waters and coastal waters around Cilacap. Abundance of larval shrimp and fish in this lagoon

  5. Fish health and fish quality

    DEFF Research Database (Denmark)

    Ingerslev, Hans-Christian

    Aquaculture is an expanding worldwide industry producing an increasing amount of fish every year. The quality of the fish meat is dependent upon many biological and non-biological factors. Infectious diseases are known to cause bleedings and damage of the muscle tissue that may lead to scarring...... are poorly described in fish. The present work in this thesis focused on: 1) examination of potential changes in the quality regarding texture of the muscle tissue in rainbow trout (Oncorhynchus mykiss) after previous infection with the bacterial pathogens Yersinia ruckeri and Vibrio anguillarum; 2...... of these studies showed that previous infections by Yersinia ruckeri and Vibrio anguillarum gave rise to subsequent changes regarding textural quality parameters in fresh fish meat, while no differences were seen for cold-smoked meat from the same fish. The texture in previous infected fish was less flaky and less...

  6. ISLAM DAN RADIKALISME: Upaya Antisipasi dan Penanggulangannya

    Directory of Open Access Journals (Sweden)

    Idrus Ruslan

    2017-02-01

    Full Text Available Sejak tragedi pemboman WTC tanggal 11 September 2001 yang disusul dengan rangkaian peledakan bom di sejumlah negara, banyak orang non Muslim yang berasumsi bahwa ajaran Islam identik dengan radikalisme. Walaupun assumsi yang berkembang itu dapat dimaklumi, namun keyakinan tersebut tentu saja tidak sepenuhnya benar. Karena meskipun terdapat sekelompok orang Islam yang berbuat radikal, akan tetapi mayoritas Muslim justru bertentangan dengan mereka. Lagi pula, jika dicermati secara mendalam, sesungguhnya Islam sama sekali tidak mentolerir tindakan radikal. Ia adalah agama kasih sayang yang sangat mendodrong penganutnya untuk berbuat baik terhadap orang lain, termasuk kepada orang-orang non-Muslim. Artikel ini menawarkan beberapa upaya strategis dalam mengantisipasi dan menanggulangi bahaya radikalisme-terorisme.

  7. Fish parasites

    DEFF Research Database (Denmark)

    This book contains 22 chapters on some of the most important parasitic diseases in wild and farmed fish. International experts give updated reviews and provide solutions to the problems......This book contains 22 chapters on some of the most important parasitic diseases in wild and farmed fish. International experts give updated reviews and provide solutions to the problems...

  8. BAHAYA RADIASI DAN CARA PROTEKSINYA

    Directory of Open Access Journals (Sweden)

    Badunggawa P

    2014-09-01

    Full Text Available Radiasi yang kita terima setiap saat, termasuk radiasi untuk tujuan kedokteran, mempunyai dampak positif dan negatif terhadap keselamatan manusia dan lingkungan. Dampak positif dari radiasi terhadap keselamatan manusia diantaranya adalah digunakan sebagai pengobatan dan dampak negatifnya adalah tergantung dari besar dosis yang diterima diantaranya adalah mulai dari mual, muntah, pusing-pusing, rambut rontok, menyebabkan kanker, diturunkan secara genetik, dan yang lebih berbahaya lagi adalah menyebabkan kematian. Oleh karena itu kita harus berhati-hati terhadap bahaya yang ditimbulkannya, baik terhadap pekerja radiasi maupun masyarakat umum termasuk pasien. Perlindungan terhadap bahaya yang ditimbulkan radiasi ini dikenal dengan istilah proteksi radiasi. Sehingga dosis yang diterima pertahun oleh pekerja atau masyarakat umum tidak melebihi batas dosis yang ditetapkan oleh Bapeten. Berbagai upaya telah dilakukan untuk meminimalkan bahaya tersebut sehingga pekerja dan pasien merasa aman melakukan dan dikenai tindakan medik.[medicina 2009;40:47-51].  

  9. EUTROFIKASI NITROGEN DAN FOSFOR SERTA PENGENDALIANNYA DENGAN PERIKANAN DI WADUK SERMO (Eutrophication by Nitrogen and Phosphorous and Its Control Using Fisheries in Sermo Reservoir

    Directory of Open Access Journals (Sweden)

    Rustadi Rustadi

    2009-11-01

    Full Text Available ABSTRAK Penelitian mengenai konsentrasi nitrogen (N dan fosfor (P dilakukan dengan tujuan untuk mengevaluasi tingkat eutrofikasi dan pencemaran air, menghitung neraca N total dan P total serta melakukan pengendaliannya menggunakan perikanan. Penelitian dilakukan menggunakan metode survai, yaitu melakukan pengambilan sampel dan pengamatan air secara purposive. Pengambilan sampel air di daerah waduk (hulu, tengah dan hilir waduk dan daerah sungai (sungai masuk dan sungai keluar. Di daerah waduk diamati pada jeluk air: 0 m, 2 m, 4 m dan dasar. Pengambilan sampel air dilakukan tiap bulan, mulai Juni 2006 sampai Mei 2007. Vanabel penelitian meliputi: amonia, amonium, nitrit, nitrat dan fosfat; N total dan P total, suhu air, kekeruhan, TDS, TSS, O, terlarut, pH, CO, bebas, alkalinitas dan plankton. Eutrofikasi air oleh N dan P dianalisis secara diskriptif dan tingkat pencemaran air dihitung menggunakan Indeks Pencemaran (lP yang disyaratkan PP RI Nomor 82. Neraca N total dan P total dihitung dengan input-output, sedangkan pengendaliannya menggunakan metode simulasi. Hasil penelitian menunjukkan bahwa eutrofikasi pada tingkat eutrofik sampai hipereutrofik, pencemaran air masih tergolong ringan di daerah budidaya ikan dan waktu konsentrasi P maksimum; budidaya ikan menggunakan KJA jaring ganda, penebaran dan pemanenan ikan di luar KJA dapat mengurangi residu dan beban N total dan P total.   ABSTRACT The research of nitrogen (N and phosphorus (P in Sermo reservoir aimed to evaluate water eutrophication and pollution, to calculate N total and P total budget and to control its concentration using fisheries. The research was conducted by direct observation and measurement on water samples taken from inflow and outflow rivers, upstream, middle and downstream areas of reservoir. The samples is reservoir were taken at water depth: 0-2,4 m and the bottom. Observation was done monthly from June 2006 up to May 2007. the variables were ammonia, ammonium, nitrite

  10. Sifat Anatomi dan Fisis Kelapa Hibrida

    OpenAIRE

    Hutabarat, Marihot Hamonangan

    2010-01-01

    Penelitian ini bertujuan untuk mengevaluasi sifat anatomis dan fisis batang kelapa hibrida. Ciri anatomi diamati langsung pada sampel dan menggunakan mikroskop untuk pengamatan dimensi seratnya, dan pengujian sifat fisis menggunakan British Standard. Sifat anatomis dan fisis batang kelapa hibrida bervariasi menurut ketinggian dan kedalaman. Struktur anatomis batang kelapa hibrida didominasi oleh vascular bundle dan parenkim. Serat kayu dapat ditemukan didalam vascular bundle dengan rata-r...

  11. Nilai Dan Fungsi Ndungndungen Karo

    OpenAIRE

    Rosita Ginting

    2010-01-01

    Tesis ini berjudul “Nilai dan Fungsi Ndungndungen Karo”. Tesis ini menjabarkan tentang macam-macam Ndungndungen Karo, nilai-nilai yang terkandung dalam Ndungndungen Karo, fungsi Ndungndungen Karo, dan bagaimana penggunaan Ndungndungen Karo pada saat sekarang ini. Metode yang dipakai dalam penelitian ini adalah metode deskriptif kualitatif, dengan teknik rekaman dan interviu. Metode kepustakaan juga digunakan dalam penelitian ini karena data sekunder dari penelitian ini adalah data-data ya...

  12. Kloning Dan Rekayasa Reproduksi

    Directory of Open Access Journals (Sweden)

    Sulchan Sofoewan

    1997-06-01

    Full Text Available REKAYASA reproduksi yang disebut Assisted Reproductive Techniques (ART atau tehnik tehnik reproduksi dengan tanpa melakukan senggama tetapi dengan bantuan secara medik, atau setiap manipulasi terhadap bagian prekreatif (reproduksi siklus kehidupan. Rekayasa reproduksi, rekayasa genetik dan kloning embrio cenderung untuk saling menunjang (sinergetik. Rekayasa genetik dan klonasi sel embrional dari hasil fertilisasi tadi dapat dilibatkan pada ART sebelum embrio ditransfer kedalam rahim. Untuk pasangan yang suaminya belum memenuhi syarat untuk fertilisasi in vitro karena jumlah spermatozoanya kurang ditempuh dengan cara menyuntikkan spermatozoa langsung kedalam ovum/oosit dengan cara mikro manipulasi/mikro injeksi yang dikenal dengan istilah lCZI (Intracytoplasmic Sperm Injection atau SUZI (Subzonal Sperm Insemination.

  13. En kamp om dans

    DEFF Research Database (Denmark)

    Zachariassen, Annette

    2004-01-01

    Hvordan er det muligt at medtænke et legende og "kæmpende" element i det faglige indholdsområde musik, bevægelse og kropsudtryk/dans? og hvordan er det muligt at lade sig inspirere af elementer fra genren elektric boogie-battel og brasiliansk kampdans "Capoeira" i tilrettelæggelse af et undervisn......Hvordan er det muligt at medtænke et legende og "kæmpende" element i det faglige indholdsområde musik, bevægelse og kropsudtryk/dans? og hvordan er det muligt at lade sig inspirere af elementer fra genren elektric boogie-battel og brasiliansk kampdans "Capoeira" i tilrettelæggelse af et...

  14. Perancangan dan Implementasi Modul Praktikum

    Directory of Open Access Journals (Sweden)

    Gita Indah Hapsari

    2015-05-01

    Full Text Available Kemajuan teknologi berbasis komputer berkembang dengan sangat pesat. Seiring dengan perkembangan jaman, kemajuan  tersebut memberikan pengaruh  terhadap  perubahan cara penyampaian pengajaran yang mengarah pada penggunaan jenis media penyampaian. Praktikum merupakan salah satu bentuk pengajaran yang memberikan pengalaman belajar secara nyata terhadap kondisi sebenarnya di lapangan mengenai penggunaan dan implementasi alat praktek yang berhubungan dengan berbagai materi yang dipelajari oleh mahasiswa dan memiliki tujuan untuk memberikan keterampilan dan pengenalan terhadap mahasiswa/peserta didik. Penelitian ini melakukan implementasi teknologi augmented reality terhadap salah satu modul praktikum yaitu pengenalan tata letak hardware dan Port I/O pada motherboard dan bertujuan sebagai alat bantu penyampaian pengajaran praktikum. Pengimplementasian teknologi augmented reality melibatkan aksi oleh user terhadap media penangkapan gambar motherboard menggunakan web camera dan tampilan pemetaan tata letak hardware dan port i/o pada motherboard. Penelitian telah diimplementasikan dan diujicoba terhadap 3 kelas dengan 97 responder. Adapun hasil dari penelitiannya adalah 83% responder menyukai aplikasi, 77% memahami materi, 84,5% menginginkan aplikasi ini menjadi alat bantu ajar, 65,98% meminta materi untuk dilengkapi dan diperbanyak, 77,3% dapat belajar mandiri menggunakan aplikasi ini, tanpa bantuan dari dosen ataupun asisten.

  15. NYERI KEPALA DAN GANGGUAN TIDUR

    Directory of Open Access Journals (Sweden)

    Lina Kamelia

    2014-09-01

    Full Text Available Nyeri kepala dan tidur merupakan dua fenomena yang saling mempengaruhi dengan patomekanisme yang kompleks. Nyeri kepala primer, terutama migren, nyeri kepala klaster dan hypnic headache dapat timbul karena pengurangan waktu tidur, parasomnia, maupun gangguan pada regulasi arsitektur tidur terutama fase rapid eye movement. Sebaliknya, adanya nyeri kepala memicu timbulnya berbagai macam gangguan tidur. Studi pencitraan otak dan biokimia menunjukkan peranan penting melatonin dan nukleus suprachiasmatik   yang mengalami disfungsi pada penderita nyeri kepala yang berhubungan dengan gangguan tidur. [MEDICINA 2013;44:101-104].

  16. SEKS DAN BUGAR

    Directory of Open Access Journals (Sweden)

    Ira Paramastri

    2016-10-01

    Full Text Available Barilah, seorang eksekutif perempuan datang ke klinik psikologi dengan keluhan "malas bahkan tidak terangsang tatkala suaminya mengajak bermain seks", atau keluhan Edo, seorang menejer muda "saya jengkel dengan diri saya sendiri karena saya tidak dapat ereksi dengan keras", atau keluhan pasangan muda wanita karir dan pengusaha Retno-Rubi "saat atau serampung hubungan seksual selalu dibarengi rasa nyeri/sakit, sungguh menyebalkan".

  17. DISTRIBUSI UKURAN TUNA HASIL TANGKAPAN PANCING LONGLINE DAN DAERAH PENANGKAPANNYA DI PERAIRAN LAUT BANDA

    Directory of Open Access Journals (Sweden)

    Umi Chodrijah

    2016-04-01

    Full Text Available Pemanfaatan sumber daya ikan tuna di perairan Laut Banda sudah berlangsung lama. Penelitian tentang komposisi jenis hasil tangkapan dan distribusi ukuran tuna hasil tangkapan longline di perairan Laut Banda yang didaratkan di Pelabuhan Benoa dilakukan pada bulan Februari, Juni, Oktober dan November 2011. Hasil penelitian menunjukkan bahwa hasil tangkapan longline yang dominan dari perairan Laut Banda adalah madidihang dan tuna mata besar. Ukuran madidihang yang tertangkap berada pada kisaran 95–165 FLcm dengan modus pada ukuran 105 FLcm dan tuna mata besar pada kisaran 75–185 FLcm dengan modus 115 dan 125 FLcm. Daerah penangkapan kapal longline di perairan Laut Banda berada pada koordinat 5–60 LS dan 124-1280 BT. Utilization of tuna resources in the Banda Sea waters had been conducting on since long time ago. Research on species composition and size distribution caught by tuna longline in the Banda Sea waters that landed in the port of Benoa  was conducted in February, June, October and November 2011. The results showed that the caught of dominant fish from longline in the Banda Sea waters were yellowfin and bigeye tuna. Size of yellowfin caught was 95–165 FLcm with a mode size of 105 FLcm and bigeye tuna was 75–185 FLcm with mode 115 and 125 FLcm. The fishing ground of longline vessels in the Banda Sea waters were 5–60 S and 124–1280 E.

  18. KEPADATAN DAN STATUS PEMANFAATAN IKAN NAPOLEON (Cheilinus undulatus DI PERAIRAN SINJAI, SULAWESI SELATAN

    Directory of Open Access Journals (Sweden)

    Amran Ronny Syam

    2016-03-01

    Full Text Available Ikan napoleon (Cheilinus undulatus adalah jenis ikan karang yang bernilai jual sangat tinggi. Hal ini menyebabkan penangkapan jenis ikan karang yang semakin langka ini menjadi cukup intensif. Saat ini populasi ikan napoleon cenderung menurun dan akan semakin sedikit jika dilakukan penangkapan tanpa batas, meskipun jenis ikan ini telah dilindungi (Appendix II CITES dan KEPMEN No.37/KEPMEN-KP/2013. Penelitian ini dilakukan dengan tujuan untuk menganalisis kepadatan populasi ikan napoleon dan status pemanfaatannya. Untuk menghitung kepadatan ikan digunakan metode sensus visual (UVC. Untuk mengetahui status pemanfaatan dilakukan sebaran frekuensi panjang ikan, yang dilanjutkan dengan penghitungan laju eksploitasi. Hasil analisis menunjukkan bahwa kepadatan ikan napoleon di sekitar perairan Sinjai (Sulawesi Selatan adalah rendah (1,8 individu/ha. Dari penghitungan laju eksploitasi, diperoleh gambaran bahwa populasi ikan napoleon di perairan sekitar Sinjai - Sulawesi Selatan telah mengalami lebih tangkap. Dari hasil ini disarankan agar perdagangan ikan napoleon masih tetap diperbolehkan dengan syarat mengikuti ketentuan ukuran dan kuota ekspor. Estimasi kuota ekspor ikan napoleon harus berdasarkan data biologi dan dinamika populasi ikan tersebut agar reproduksi alamiah ikan napoleon dapat berlangsung seimbang dengan tingkat eksploitasinya. Napoleon wrasse (Cheilinus undulatus is a group of reef fish that is  high economic value. This led to the capture of reef fish species that is becoming increasingly scarce intensive enough. Currently napoleon fish populations tend to decline and will be less if the arrest was without limit, although this fish species has been protected (Appendix II of CITES and KEPMEN 37/KEPMEN-KP/2013. This study was conducted to analyze the population density of napoleon wrasse and utilization status. The density of fish used snorkeling visual census method (UVC. To find out the status of the utilization used the frequency

  19. Protocols for Pachytene Chromosome and DNA Fiber FISH in Cotton

    Institute of Scientific and Technical Information of China (English)

    PENG Ren-hai; LING Jian; WANG Kun-bo; WANG Chun-ying; SONG Guo-li; LIU Fang

    2008-01-01

    @@ Fluorescence in situ hybridization (FISH) has become the most important technique in plant molecular cytogenetics research.FISH-based physical mapping provides a valuable complementary approach in genome sequeneing to measure the physical distances between adjacent BAC contigs and delineating the structure and DNA composition of genomic regions of centromere and telomere.The accuracy and precision of the FISH-base physical maps depend on the resolution power of FISH.

  20. ASIMETRI INFORMASI DAN UNDERPRICING

    Directory of Open Access Journals (Sweden)

    Tety Anggita Safitri

    2013-03-01

    Full Text Available Tujuan penelitian ini adalah untuk menguji pengaruh asimetri informasi terhadap underpricing. Penelitian ini menggunakan sampel 63 perusahaan yang melakukan penawaran umum perdana di Bursa Efek Indonesia dalam kurun waktu 2005-2010. Analisis data menggunakan regresi linier berganda, yaitu menguji proksi asimetri informasi yang terdiri atas ukuran perusahaan, umur perusahaan, proporsi saham yang ditawarkan kepada masyarakat, reputasi underwriter dan reputasi auditor terhadap underpricing. Hasil penelitian ini mengindikasikan bahwa reputasi underwriter dan reputasi auditor berpengaruh terhadap underpricing. Ukuran perusahaan, umur perusahaan dan proporsi saham yang ditawarkan tidak berpengaruh terhadap underpricing.The aim of this research is to examine the effect of assymetric information on underpricing. This research used a sample of 63 companies that make initial public offering on the Indonesia Stock Exchange in the period of 2005-2010. The data analysis is using multiple linear regression, which is testing the proxy of asymmetric information which consists of the firm size, the firm age, the proportion of shares offered to the public, underwriter reputation and auditor reputation on underpricing. This research indicates that underwriter reputation and auditor reputation have a significant effect on underpricing. The firm size, the firm age and the proportion of shares offered to the public have no significant effect on underpricing.

  1. ASIMETRI INFORMASI DAN UNDERPRICING

    Directory of Open Access Journals (Sweden)

    Tety Anggita Safitri

    2013-03-01

    Full Text Available Tujuan penelitian ini adalah untuk menguji pengaruh asimetri informasi terhadap underpricing. Penelitian ini menggunakan sampel 63 perusahaan yang melakukan penawaran umum perdana di Bursa Efek Indonesia dalam kurun waktu 2005-2010. Analisis data menggunakan regresi linier berganda, yaitu menguji proksi asimetri informasi yang terdiri atas ukuran perusahaan, umur perusahaan, proporsi saham yang ditawarkan kepada masyarakat, reputasi underwriter dan reputasi auditor terhadap underpricing. Hasil penelitian ini mengindikasikan bahwa reputasi underwriter dan reputasi auditor berpengaruh terhadap underpricing. Ukuran perusahaan, umur perusahaan dan proporsi saham yang ditawarkan tidak berpengaruh terhadap underpricing.The aim of this research is to examine the effect of assymetric information on underpricing. This research used a sample of 63 companies that make initial public offering on the Indonesia Stock Exchange in the period of 2005-2010. The data analysis is using multiple linear regression, which is testing the proxy of asymmetric information which consists of the firm size, the firm age, the proportion of shares offered to the public, underwriter reputation and auditor reputation on underpricing. This research indicates that underwriter reputation and auditor reputation have a significant effect on underpricing. The firm size, the firm age and the proportion of shares offered to the public have no significant effect on underpricing.

  2. DIVERSITAS DAN KERAPATAN MANGROVE, GASTROPODA DAN BIVALVIA DI ESTUARI PERANCAK, BALI

    OpenAIRE

    2011-01-01

    Penelitian ini bertujuan membandingkan diversitas dan kerapatan mangrove dengan kepadatan gastropoda dan bivalvia di mangrove alami dan rehabilitasi. Pengukuran ekosistem mangrove menggunakan transek kuadrat 10 m x 10 m. Kelimpahan dan kepadatan gastropoda dan bialvia menggunakan transek kuadrat berukuran 1 m x 1 m. Analisis nMDS, cluster untuk melihat hubungan karekteristik mangrove alami dan rehabilitasi dianalisis secara deskriptif dan analisis regresi untuk mendetermi...

  3. KARAKTERISASI FENOTIPE DAN GENOTIPE TIGA POPULASI IKAN TENGADAK (Barbonymus schwanenfeldii

    Directory of Open Access Journals (Sweden)

    Irin Iriana Kusmini

    2017-01-01

    Full Text Available Ikan tengadak, Barbonymus schwanenfeldii merupakan salah satu jenis ikan lokal yang potensial untuk dikembangkan. Kemungkinan keberhasilan pengembangan ikan ini sangat tinggi karena ikan tengadak sekerabat dengan ikan tawes. Studi keragaman genetik dilakukan dalam upaya pemanfaatan sumber daya genetik untuk kegiatan budidaya secara berkelanjutan. Tujuan penelitian ini adalah untuk menganalisis keragaman dan kekerabatan antara populasi ikan tengadak asal Jawa, Sumatera, dan Kalimantan. Analisis fenotipe dilakukan berdasarkan pengukuran truss-morfometrik, sedangkan analisis genotipe dilakukan secara molekuler menggunakan metode PCR-RAPD dengan primer OPA-08, OPA-09, dan OPC-02. Hasil analisis fungsi kanonikal truss-morfometrik menunjukkan sebaran pengukuran ketiga populasi berada pada kuadran yang berbeda dengan persentase indeks keseragaman tertinggi pada populasi Sumatera dan Jawa (100%. Analisis genotipe menunjukkan ikan tengadak populasi Kalimantan memiliki nilai polimorfisme (50,00 dan heterozigositas tertinggi (0,206; secara kekerabatan ikan tengadak Kalimantan memiliki jarak yang jauh dengan ikan tengadak Jawa dan Sumatera. Tinfoil barb Barbonymus schwanenfeldii is one of potential local fish species for aquaculture development. The successful probability of aquaculture development of tinfoil barb is very high due to close genetic relationship with silver barb. Study related to genetic diversity was conducted in order to explore genetic resources for sustainable aquaculture. The aim of this study was to analyze the genetic diversity and relationship between tinfoil barb populations from Java, Sumatra, and Kalimantan. Phenotype analysis was conducted by truss morphometric, whereas genotype analysis conducted by PCR-RAPD using OPA-08, OPA-09, and OPC-02 primers. The results of canonical correlation analysis from truss-morphometric measurements showed that the distribution of three populations were located in different quadrants with the

  4. One Fish, Two Fish, Redfish, You Fish!

    Science.gov (United States)

    White, Katherine; Timmons, Maryellen; Medders, Paul

    2011-01-01

    The recreational fishing activity presented in this article provides a hands-on, problem-based experience for students; it unites biology, math, economics, environmental policy, and population dynamics concepts. In addition, the activity allows students to shape environmental policy in a realistic setting and evaluate their peers' work. By…

  5. IJTIHAD KONTEMPORER (Problem dan Solusinya

    Directory of Open Access Journals (Sweden)

    Mustaqim Mustaqim

    2011-04-01

    Full Text Available Ijtiha>d adalah merupakan bahasan yang tak henti-hentinya menjadi kajian ketat para ulama sejak dulu sampai kini, seperti imam al-Ghaza>li> membahas dalam bukunya al mustasfa, demikian juga imam assyaukani membahas dalam bukunya irsyadul puhul dan masih banyak lagi ulama yung membahas dalam masalah ijtiha>d ini, Begitu juga dari kalangan ulama kontemporer seperti, Shaykh Ah}mad Ibra>hi>m, Shaykh ‘Abd al-Wahha>b Khala>f, Dr. Yu>suf al-Qarad}awi>, Dr. T{a>ha> Jabi>r dan masih banyak lagi selain mereka. Bersama itu, pintu ijtiha>d tak pernah tertutup, karena kehidupan masih terus berlanjut, dan kejadian-kejadian masih terus berkembang, timbulnya krisis ekonomi dan sosial, rangkaian masalah dan berbagai macam solusinya, desakan kejadian yang tak pernah dipersiapkan oleh manusia yang menimbulkan aneka ragam kesulitan, dan terus mengakar sampai menyelami kehidupan tekhnologi, perdagangan, atau kehidupan individu dan umum. Berawal dari sini maka merupakan cambuk bagi seorang muslim dengan kebebasan berpikirnya untuk mengerahkan segala kemampuannya terhadap seluruh masalah, sebab islam adalah agama kehidupan yang mencakup segala aspek, Islam bukan hanya berurusan dengan masalah akhirat saja, tapi Islam lahir dalam bentuk yang luas, mampu mengobati penyakit sosial, ekonomi, internasional, dll, dan fiqih sendiri bukan hanya merupakan peraturan belaka, melainkan fiqih mencakup segala masalah tergantung kejadian dan kebutuhan, fiqih masih siap menerima perubahan selama masih tetap dalam rel qur’an dan sunnah, dari sinilah perlu adanya ijtiha>d, Ijtiha>d merupakan hal yang terbuka bagi seorang muslim agar merasakan kebebasan berfikir yang sempurna karena ijtiha>d adalah merupakan sebuah bukti akan luas dan mudahnya syariat islam.

  6. INTERAKSI TROFIK JUVENIL IKAN DAN UDANG DI LAGUNA SEGARA ANAKAN, CILACAP

    Directory of Open Access Journals (Sweden)

    Didik Wahju Hendro Tjahjo

    2016-05-01

    Full Text Available Segara Anakan mempunyai peran penting sebagai kawasan pengelolaan sumber daya ikan dan udang. Beberapa jenis ikan dan udang menggunakan laguna ini sebagai daerah asuhan. Penelitian dengan tujuan untuk mengetahui aspek biologi juvenil ikan dan udang dalam memanfaatkan sumber daya makanan alami dilakukan di perairan laguna Segara Anakan Kabupaten Cilacap pada tahun 2010. Pengambilan sampel dilakukan dengan menggunakan metode tersratifikasi dengan menetapkan 9 stasiun pengamatan. Analisis data meliputi kebiasaan makan, tingkat trofik, luas relung serta interaksi juvenile ikan dan udang dalam memanfaatkan sumber daya makanan alami. Hasil analisis kebiasaan makan menunjukkan bahwa juvenil ikan dan udang dapat dikelompokan menjadi tiga tingkatan trofik yaitu herbivora (2,00 – 2,50, omnivora (2,51 – 3,00 dan karnivora (3,01 – 3,50.  Luas relung makanan juvenil ikan dan udang adalah relatif sempit atau bersifat spesialis dengan nilai indeks berkisar antara 1,00 – 3,02. Hasil analisis kesamaan dalam pemanfaatan sumber daya makanan menunjukkan bahwa interaksi antar jenis ikan dan udang relatif rendah, sehingga peluang terjadinya kompetisi antar jenis rendah. Kondisi ini dapat diartikan bahwa ketersediaan makanan alami di perairan laguna tidak menjadi faktor pembatas.   Segara Anakan loogon plays an important role as the management area of fish and shrimp resources. Several species of fish and shrimp use this lagoon as an area of nursery ground. A research aimed to investigate the biological aspects of juvenile fish and shrimps in the utilization of natural food resources. Have been conductcd in the lagoon of Segara Anakan, Cilacap District in 2010. Sampling was done by using a stratification method with 9 research stations. Food habits, trophic level, niche width and interactions in the utilization of natural food resources were analyzed. Results showed that the juveniles of fish and shrimp could be grouped into three trophic levels i

  7. Fighting fish

    Science.gov (United States)

    Duchi, E.; Guerrini, V.; Rinaldi, S.; Schaeffer, G.

    2017-01-01

    We introduce new combinatorial structures, called fighting fish, that generalize directed convex polyominoes by allowing them to branch out of the plane into independent substructures. On the one hand the combinatorial structure of fighting fish appears to be particularly rich: we show that their generating function with respect to the perimeter and number of tails is algebraic, and we conjecture a mysterious multivariate equidistribution property with the left ternary trees introduced by Del Lungo et al On the other hand, fighting fish provide a simple and natural model of random branching surfaces which displays original features: in particular, we show that the average area of a uniform random fighting fish with perimeter 2n is of order n 5/4: to the best of our knowledge this behaviour is non-standard and suggests that we have identified a new universality class of random structures. Dedicated to Tony Guttmann on the occasion of his 70th birthday.

  8. ANALISIS JENIS, JUMLAH, DAN MUTU GIZI KONSUMSI SARAPAN ANAK INDONESIA

    Directory of Open Access Journals (Sweden)

    Fachruddin Perdana

    2013-11-01

    Full Text Available ABSTRACTThe objective of this study was to analyze type, amount, and nutritional quality of breakfast among Indonesian children aged 3—12 years old. Data used for this study was secondary data of Basic Health Survey 2010 (Riskesdas 2010. Data was collected from June until August 2010 by applying a cross sectional design. This study covered 33 provinces of Indonesia, with total 40,437 children consisted of 20,659 boys and 19,778 girls. The results of this study showed that ten major type of foods consumed during breakfast were rice, swamp cabbage, egg, fish, tempe, instant noodles, tofu, bread, chicken and biscuits; and five major type of beverages consumed during breakfast were drinking water, tea, milk, coffee, and syrup. The food consumed on average of more than 5 g a day during breakfast were rice, swamp cabbage, egg, fish, tempe, instant noodles; and the beverages consumed on average of more than 15 mL a day were water, tea, and milk. Only 10.6% of children breakfast met energy intake>30% RDA.Keywords: breakfast, breakfast amount, breakfast type, children, nutritional quality of breakfastABSTRAKTujuan dari penelitian ini adalah untuk menganalisis jenis, jumlah, dan mutu gizi konsumsi sarapan anak Indonesia usia 3—12 tahun. Data yang digunakan untuk penelitian ini adalah data sekunder (data konsumsi pangan yang diperoleh dari hasil penelitian Riset Kesehatan Dasar 2010 (Riskesdas 2010 yang dilaksanakan oleh Badan Penelitian dan Pengembangan Kesehatan, Kementrian Kesehatan Indonesia. Pengumpulan data dilakukan pada bulan Juni sampai Agustus 2010 dengan menggunakan desain cross sectional. Penelitian ini mencakup 33 provinsi di Indonesia, dengan 40 437 total subjek; yang terdiri dari 20 659 laki-laki dan 19 778 perempuan. Hasil penelitian menunjukkan bahwa sepuluh jenis makanan yang paling banyak dikonsumsi selama sarapan adalah nasi, kangkung, telur ayam, ikan, tempe, mi instan, tahu, roti, daging ayam, dan biskuit; lima jenis minuman yang

  9. Desain Multi-purpose Support Ship sebagai Sarana Pengamanan, Pemetaan, dan Pusat Komando untuk Meningkatkan Keamanan Perairan Indonesia

    Directory of Open Access Journals (Sweden)

    Dewangga Aradea Widjatmiko

    2017-03-01

    Full Text Available Akibat maraknya pelanggaran-pelanggaran seperti illegal fishing, perompakan, penyelundupan, dan pelanggaran wilayah perbatasan laut Sulawesi, Indonesia. maka dibutuhkan kapal yang memiliki beragam kemampuan yaitu sebagai kapal kombatan (anti kapal permukaan, anti kapal selam, dan anti serangan udara, kapal markas (command ship, kapal hidro-oseonagrafi (kapal survey dan riset, kapal bantu angkut personel dan kendaraan tempur (support ships, dan kapal pengawas lingkungan (environmental protection. Jenis kapal yang dibuat adalah Multi-purpose Support Ship (MSS, dikarenakan kegunaan yang dapat diubah sesuai dengan kebutuhannya. Sehingga diharapkan dapat meningkatkan keamanan laut Indonesia. Dari proses desain ini didapat ukuran Multi-purpose Support Ship yaitu; Lpp = 110.0 m, B =14 m, H = 6.5 m, T= 4,5 m.

  10. Epidermal Growth Factor Receptor (EGFR gene copy number (GCN correlates with clinical activity of irinotecan-cetuximab in K-RAS wild-type colorectal cancer: a fluorescence in situ (FISH and chromogenic in situ hybridization (CISH analysis

    Directory of Open Access Journals (Sweden)

    Scartozzi Mario

    2009-08-01

    Full Text Available Abstract Background K-RAS wild type colorectal tumors show an improved response rate to anti-EGFR monoclonal antibodies. Nevertheless 70% to 40% of these patients still does not seem to benefit from this therapeutic approach. FISH EGFR GCN has been previously demonstrated to correlate with clinical outcome of colorectal cancer treated with anti-EGFR monoclonal antibodies. CISH also seemed able to provide accurate EGFR GCN information with the advantage of a simpler and reproducible technique involving immunohistochemistry and light microscopy. Based on these findings we investigated the correlation between both FISH and CISH EGFR GCN and clinical outcome in K-RAS wild-type colorectal cancer treated with irinotecan-cetuximab. Methods Patients with advanced K-RAS wild-type, colorectal cancer receiving irinotecan-cetuximab after failure of irinotecan-based chemotherapy were eligible. A cut-off value for EGFR GCN of 2.6 and 2.12 for FISH and CISH respectively was derived from ROC curve analysis. Results Forty-four patients were available for analysis. We observed a partial remission in 9 (60% and 2 (9% cases with a FISH EGFR GCN ≥ 2.6 and Conclusion FISH and CISH EGFR GCN may both represent effective tools for a further patients selection in K-RAS wild-type colorectal cancer treated with cetuximab.

  11. [Progress in transgenic fish techniques and application].

    Science.gov (United States)

    Ye, Xing; Tian, Yuan-Yuan; Gao, Feng-Ying

    2011-05-01

    Transgenic technique provides a new way for fish breeding. Stable lines of growth hormone gene transfer carps, salmon and tilapia, as well as fluorescence protein gene transfer zebra fish and white cloud mountain minnow have been produced. The fast growth characteristic of GH gene transgenic fish will be of great importance to promote aquaculture production and economic efficiency. This paper summarized the progress in transgenic fish research and ecological assessments. Microinjection is still the most common used method, but often resulted in multi-site and multi-copies integration. Co-injection of transposon or meganuclease will greatly improve the efficiency of gene transfer and integration. "All fish" gene or "auto gene" should be considered to produce transgenic fish in order to eliminate misgiving on food safety and to benefit expression of the transferred gene. Environmental risk is the biggest obstacle for transgenic fish to be commercially applied. Data indicates that transgenic fish have inferior fitness compared with the traditional domestic fish. However, be-cause of the genotype-by-environment effects, it is difficult to extrapolate simple phenotypes to the complex ecological interactions that occur in nature based on the ecological consequences of the transgenic fish determined in the laboratory. It is critical to establish highly naturalized environments for acquiring reliable data that can be used to evaluate the environ-mental risk. Efficacious physical and biological containment strategies remain to be crucial approaches to ensure the safe application of transgenic fish technology.

  12. Analisis dan Optimasi Desain Sistem Reaktor Gas Temperatur Tinggi RGTT200K dan RGTT200KT

    Directory of Open Access Journals (Sweden)

    Mohammad Dhandhang Purwadi

    2013-03-01

    dengan penyelesaian persamaan aljabar linier dari model RGTT200K dan RGTT200KT. Dari analisis dan optimasi ini dihasilkan desain konseptual sistem RGTT200K dan RGTT200KT dengan spesifikasi thermal-flow teras sama, dan peningkatan EUF dari 63% menjadi 80,14% (untuk RGTT200K dan dari 63,6% menjadi 78,02% (untuk RGTT200KT.

  13. Fluorescent microspheres

    Science.gov (United States)

    Rembaum, A.

    1978-01-01

    Latex particles with attached antibodies have potential biochemical and environmental applications. Human red blood cells and lymphocytes have been labeled with fluorescent microspheres by either direct or indirect immunological technique. Immunolatex spheres can also be used for detecting and localizing specific cell surface receptors. Hormones and toxins may also be bondable.

  14. OPTIMASI JUMLAH RUMPON, UNIT ARMADA DAN MUSIM PENANGKAPAN PERIKANAN TUNA DI PERAIRAN PRIGI, JAWA TIMUR

    Directory of Open Access Journals (Sweden)

    Erfind Nurdin

    2016-05-01

    Full Text Available Sebagai alat bantu penangkapan ikan, rumpon berfungsi untuk menarik kelompok ikan agar berkumpul di sekitarnya. Dalam jangka pendek rumpon dapat meningkatkan produksi hasil tangkapan, efisiensi dan efektivitas operasi penangkapan ikan. Namun rumpon juga dapat berdampak negatif terhadap keberlajutan stok sumberdaya. Penelitian ini dilakukan di PPN Prigi, Jawa Timur, dengan tujuan untuk mengkaji status pemanfaatan perikanan tuna, optimasi jumlah unit armada dan rumpon serta musim penangkapan ikan.  Beberapa analisis yang digunakan antara lain linear goal programming (LGP, fishing power indeks (FPI, catch per unit of effort (CPUE, maximum sustainable yield (MSY, dan untuk mengetahui pola musim tangkap menggunakan Metode Persentase Rata-rata. Hasil penelitian menunjukkan terdapat indikasi pemanfaatan perikanan tuna yang berlebih pada tingkat pengupayaan yang melampaui batas maksimum (MSY = 2334,9 ton/tahun.  Jumlah optimum untuk armada jaring insang sebanyak 43 unit, pancing tonda 63 unit dan rumpon 33 unit pada luasan area penelitian 8.940 km². Musim tangkap berlangsung pada Bulan Juni sampai Desember dengan puncak musim di bulan Juli.    Fish Aggregating Device (FADs has a function to attract and aggregate fish schooling. In short term, the advantage of FADs used is to increase the efficiency and effectiveness of fishing operations and the fish caught by the fishers; however FADs might also result a negative impact on the sustainability of fish stock.This study was conducted in fishing area of Prigi National Fishing Port, East Java. The objective of this study is to investigate the tuna fisheries status, optimization number of fishing units and number of FADs. Some analysis methods applied in this study were linear goal programming (LGP, fishing power index (FPI, catch per unit of effort (CPUE, maximum sustainable yield (MSY, and analysis of fishing season using the Average Percentage Methods. The results showed that the tuna fisheries in

  15. Generation and characterization of a stable red fluorescent ...

    African Journals Online (AJOL)

    AJL

    2012-04-12

    Apr 12, 2012 ... such as the fluorescent transgenic zebrafish (Danio rerio) and Japanese medaka ..... number in transgenic animals. Biotechniques, 37: ... Development of transgenic fish for ornamental and bioreactor by strong expression.

  16. Virginia ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and brackishwater fish species in Virginia. Vector polygons in this data...

  17. Hawaii ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for reef, marine, estuarine, and native stream fish species in coastal Hawaii. Vector polygons in this data...

  18. Alabama ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, and freshwater fish species in Alabama. Vector polygons in this data set represent...

  19. Louisiana ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for freshwater (inland) fish species in coastal Louisiana. Vector polygons represent water-bodies and other...

  20. Exfoliative Cheilitis dan Penatalaksanaannya

    Directory of Open Access Journals (Sweden)

    Dewi Agustina

    2012-12-01

    Full Text Available Latar Belakang: Exfoliative cheilitis adalah suatu keadaan inflamatori kronis superfisisal yang ditandai dengan adanya pengelupasan permukaankeratin bibir sedangkan area yang lain terjadi pembentukan lapisan keratin. Tujuan: untuk melaporkan suatu  kasus Exfoliative cheilitis yang diikuti +  selama 2 bulan beserta penatalaksanaanya. Laporan kasus: seorang wanita berusia 52 tahun mengeluh bibir bawahnya pecah-pecah, terasa kering dan panas berdasarkan pemeriksaan subyektif dan klinis, Exfoliative cheilitis ditetapkan sebagai diagnosis kerja. Penatalaksanaanya: Komunikasi-Informasi-Edukasi (KIE diberikan kepada pasien pada awal kunjungan disertai pemberian metil prednisolon, Vitamin B1,6,12 dan anjuran untuk menghentikan kebiasaan menjilat-jilat bibir bawah, menghindari stress dan meningkatkan asupan makanan yang mengandung vitamin A. Pada kunjungna berikutnya pemberian metil prednisolon dihentikan karena terdeteksi adanya peningkatan tekanan darah. Untuk selanjutnya kondisi bibir bawah dievaluasi +  selama 2 bulan. Kesimpulan: Progresitas Exfoliative cheilitis dapat dihentikan dengan bantuan KIE serta menghindari faktor pemicu stress, yang pada akhirnya akan dapat meminimalisir aktivitas factitious berupa menjilat-jilat bibir bawah.    Background: Exfoliative cheilitis is a chronic superficial inflammatory condition that is characterized by regular peeling of a superficial excessive layer of karatin, on the other hand, keratinization is developed in the other area. Aim: to report an Exfoliative cheilitis case monitored for two months and its management. Case Report: a 52 year female has been suffering from fissured lower lip with dry and burning sensations. According to subjective and clinical examinations, Exfoliative cheilitis was determined as the working diagnosis. Management: Communication-Information-Education (CIE were given to the patient at the first visit, besides metyl prednisolon and vitamin B1,6,12 administrations. Patient was

  1. PENGARUH CURAH HUJAN TERHADAP KONDISI PERAIRAN DAN HASIL TANGKAPAN IKAN DI ESTUARI SUNGAI BARITO

    Directory of Open Access Journals (Sweden)

    Aroef Hukmanan Rais

    2015-09-01

    Full Text Available Curah hujan mempengaruhi aktifitas penangkapan dan juga lingkungan perairanan di sekitarnya. Tujuan penelitian ini adalah untuk melihat pengaruh curah hujan terhadap produksi tangkapan dan lingkungan perairan estuari sungai Barito. Pengambilan sampel hasil tangkapan dilakukan oleh bantuan enumerator alat tangkap lampara, tuguk dan rawai selama 7 bulan dari bulan Maret hingga September 2012. Parameter kondisi perairan yang diambil adalah nitrit, amoniak, total fosfat, kesadahan, turbiditas, daya hantar listrik (DHL , kecerahan, oksigen, suhu, salinitas dan pH. Produksi hasil tangkap dihitung dalam nilai catch per unit effort (CPUE. Uji masing-masing parameter terhadap curah hujan menggunakan uji t, sedangkan model persamaan CPUE terhadap curah hujan dan kondisi perairan diperoleh melalui regresi berganda motode backward. Puncak musim penghujan pada saat penelitian terjadi pada bulan Maret dan pucak musim kemarau terjadi pada bulan September. Hasil uji – t pengaruh curah hujan dan parameter kondisi perairan menunjukan nilai signifikan pada parameter daya hantar listrik (DHL. Hubungan antara curah hujan dan nilai CPUE menunjukan nilai yang signifikan berbeda nyata, dimana nilai CPUE cenderung meningkat pada musim kemarau. Model regresi berganda CPUE yang terbentuk menunjukan persamaan yang signifikan terhadap parameter curah hujan dan oksigen terlarut. The influences of rainfall rate on fishing activities and the waters environment condition. This research has purpose to saw rainfall rate effect on fishing production and waters environment of Barito river estuary. Sampel of fishing result has collected by enumerator of lampara, tuguk, and rawai for 7 months, from March until September 2012. Parameters of water condition have collected are nitric, ammoniac, fosfat total, hardness, turbidity, conductivity, transparence, oxygen, temperature, salinity and pH. Fish production is showed by catch per unit effort (CPUE. Test for each water parameters and

  2. Cloning of fish enzymes and other fish protein genes.

    Science.gov (United States)

    Macouzet, M; Simpson, B K; Lee, B H

    1999-01-01

    Fish metabolism needs special enzymes that have maximum activity at very different conditions than their mammalian counterparts. Due to the differences in activity, these enzymes, especially cold-adapted proteases, could be used advantageously for the production of some foods. In addition to the enzymes, this review describes some other unique fish polypeptides such as antifreeze proteins, fluorescent proteins, antitumor peptides, antibiotics, and hormones, that have already been cloned and used in food processing, genetic engineering, medicine, and aquaculture. Recombinant DNA technology, which allows these biological molecules to be cloned and overexpressed in microorganisms is also described, highlighting innovative applications. The expected impact of cloning fish proteins in different fields of technology is discussed.

  3. Singkatan Dan Akronim Dalam Chatting Di Internet

    OpenAIRE

    Hervina M. S.

    2010-01-01

    Penelitian ini menganalis pola singkatan dan akronim dalam chatting di internet. Tujuan penelitian ini adalah mendeskripsikan pola singkatan dan akronim dalam chatting di internet dan mendeskripsikan pola singkatan dan akronim yang paling dominan digunakan dalam chatting di internet. Teori yang digunakan adalah singkatan, akronim, bahasa dalam chatting, sosiolinguistik. Pengumpulan data digunakan dengan metode simak, yaitu penyimakan terhadap penggunaan bahasa (Sudaryanto,1993:133), dan tekni...

  4. LINTASAN SEJARAH FILSAFAT PENDIDIKAN PERENIALISME DAN AKTUALISASINYA

    OpenAIRE

    2015-01-01

    Antara filsafat dan pendidikan terdapat kaitan yang erat. Filsafat memiliki aspek-aspek utama yang dapat dijadikan landasan bagi pendidikan. Aspek-aspek yang dimaksud adalah aspek-aspek metafisis, epistemologis, dan aksiologis. Aspek metafisis antara lain berkaitan dengan persoalan realitas yang tercermin pada bahan ajar, pengalaman dan keterampilan. Aspek epistemologis berkaitan dengan persoalan pengetahuan dan kebenaran, termasuk di dalamnya sumber belajar dan metode pembelajaran. Aspek aks...

  5. PEMBUATAN DAN KARAKTERISASI ARANG DARI BATANG TANAMAN GUMITIR (Tagetes erecta) PADA BERBAGAI SUHU DAN WAKTU PIROLISIS

    National Research Council Canada - National Science Library

    I Made Siaka; Ni Putu Diana Febriyanti; Emmy Sahara; I Made Sutha Negara

    2017-01-01

    ...) pada berbagai suhu dan waktu pirolisis. Penelitian ini dilakukan untuk memperoleh suhu dan waktu yang optimum dalam pembuatan arang serta mengetahui karakteristik arang yang dihasilkan pada suhu dan waktu optimumnya...

  6. RESISTENSI AGAMA DAN BUDAYA MASYARAKAT

    Directory of Open Access Journals (Sweden)

    David Samiyono

    2013-12-01

    Interaksi antar etnis, agama dan budaya bukanlah barang langka di Bali. Sejak semula hal tersebut sudah ada, ketika pulau ini menjadi locus perdagangan hasil bumi. Sebab sesungguhnya kebudayaan Bali menjujung tinggi nilai-nilai keseimbangan dan harmonisasi antar manusia dengan Tuhan (perhyangan, dengan sesama (pawongan dan dengan lingkungan (palemahan. Konsep ini disebut Tri hita karana. Bali kini berubah. Budayanya berorientasi pada jasa, yang berkait dengan industri pariwisata. Sikap orang Bali kini tidak lagi ramah dan harmoni. Akibat ledakan bom dalam bulan Oktober 2002 dan 2005, masyarakat lebih berhati-hati terhadap para pendatang. Ajeg Bali merupakan kearifan lokal –agama dan budaya– masyarakat Bali dalam rangka menanggulangi pengaruh luar yang mengakibatkan perubahan di berbagai bidang sehingga identitas kebalian mengalami degradasi. Ajeg Bali merupakan bentuk resistensi masyar­akat Bali dalam rangka membatasi pendatang dari luar Bali. Kajian ini berusaha untuk menjawab pertanyaan “apakah Ajeg Bali, sebuah kearifan lokal masyarakat Bali dapat membendung pengaruh budaya, agama dan ekonomi masyarakat Bali?”

  7. Fluorescent in situ hybridization as an adjunct to conventional cytogenetics.

    Science.gov (United States)

    Mark, H F

    1994-01-01

    Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique that exploits the availability of recombinant deoxyribonucleic acid (DNA) technology. In metaphase FISH, a specific nucleic acid sequence (probe) is bound to the homologous segment on a metaphase chromosome in a fixed preparation on a glass slide. The presence of a region-specific DNA sequence in a nondividing cell can be detected using interphase FISH. Interphase cytogenetics via FISH can be performed on fixed cells harvested during a routine culture, on tissue sections and on many cytologic specimens. Specific examples of clinical and research applications are discussed to illustrate the utility of FISH in the detection of constitutional and acquired chromosomal abnormalities.

  8. RECOVERY OF THIRD-STAGE LARVAE OF ANGUILLICOLA CRASSUS (NEMATODA : DRACUNCULOIDEA FROM THE FLESH OF FISHES : ASSESSMENT OF A DIGESTIVE MEDIUM AS A TOOL FOR THE STUDY OF FISH PARATENIC HOSTS. EXTRACTION DE LARVES DE TROISIÈME STADE D'ANGUILLICOLA CRASSUS (NEMATODA : DRACUNCULOIDEA DANS LA CHAIR DE POISSONS : MISE AU POINT D'UNE MÉTHODE PAR DIGESTION POUR L'ÉTUDE DU PORTAGE PARASITAIRE PAR LES POISSONS HÔTES PARATÉNIQUES.

    Directory of Open Access Journals (Sweden)

    AGOULON A.

    2008-05-01

    Full Text Available The purpose of this study was to recover third-stage larvae (L3 of Anguillicola crassus alive from the flesh of fishes. Different digestive media have been tested on eel flesh for their efficiency and on living infective third-stage larvae for their harmlessness. The results were : (1 Long-term survival of the L3 larvae was low, but their morphological features were recognizable in each digestion runs. (2 Pepsin concentrations between 2.5 and 40 g/l proved to be without influence on the short-term (2-4 hours survival of the L3 larvae of A. crassus. The digestion of the eel flesh was more advanced with the most concentrated media, when applied for 12 hours. (3 L3 larvae survived longer at pH 1.8 than 1.5, and digestion appeared to be faster at the upper pH level. (4 L3 larvae survival was much longer at 20°C than at 30°C, but efficiency of the digestion process was poor at the lower temperature. For epidemiological studies, the optimal factors combination has been established at pepsin concentration : 10 g/l, pH : 1.8 and temperature : 20-22°C, particularly when quantitative recovery of L3 larvae and work efficiency were taken into account. Le but de cette étude était de récupérer les larves de troisième stade (L3 d'Anguillicola crassus au sein de la chair de différents poissons. Neuf milieux de digestion ont été testés sur de la chair d'anguille, pour évaluer leur efficacité lytique. Ces mêmes milieux ont été testés sur des larves L3 vivantes d' A. crassus pour apprécier leur innocuité sur le parasite. Les résultats sont les suivants : (1 La survie à long terme des larves L3 a été limitée, mais leur intégrité anatomique n'a pas été altérée, de sorte qu'elles sont restées identifiables dans chaque milieu testé. (2 Aucune différence de survie à court terme (2-4 heures n'a été constatée sur les larves selon la concentration en pepsine (2,5 ou 40 g/l. La concentration la plus élevée en pepsine a assuré une

  9. RAPID ASSESMENT LIMNOLOGIS SEBAGAI INDIKATOR PENGELOLAAN (REGULASI DAN PEMANFAATAN PERAIRAN DI ZONA PENYANGGA LAHAN BASAH RESERVAT DANAU PANGGANG KABUPATEN HULU SUNGAI UTARA, KALIMANTAN SELATAN

    Directory of Open Access Journals (Sweden)

    Pathul Arifin

    2017-02-01

    Full Text Available Penurunan jumlah tangkapan di perairan reservat danau panggang kabupaten hulu sungai utara sebesar 2% pertahun dari 3145,1 ton (2010 menjadi 3.030,0 ton (2013 dan kehadiran ikan lais (Cryptopterus micronema < 1% setiap tahunnya disusul ikan baung (Mystus nemurus < 0,5% mengindikasikan penurunan kualitas air, produktivitas perairan dan lingkungan perairan secara keseluruhan sebagai sistem ekologi. Tujuan penelitian (a mengidentifikasi kondisi limnologis perairan di zona penyangga kawasan reservat danau panggang dengan analisis kualitas air dan biological indeks; (b menganalisis konfigurasi ruang dari aktivitas di lokasi dan nilai sumberdaya perairan dari karakter bioekologis; (c menilai kondisi keseluruhan atribut perairan di zona penyangga; (d memetakan profil zonasi untuk menjelaskan kondisi eksisting di lokasi penelitian. Metode analisis kualitas air dan biological indeks serta kuissioner disertai statistik deskriptif dan pemetaan. Konsentrasi bahan organik yang tinggi berasal dari limbah organik dan tingkat biodiversitas fitoplankton lebih dominan daripada zooplankton berupa kelompok Chlorophyta air tawar sebagai pakan alami bagi larva dan benih ikan. Struktur pemukiman di lokasi terhadap perairan 50-75%, pembudidaya dan nelayan sebagai profesi utama dengan 2-10 jenis ikan yang tertangkap serta 4 jenis yang kehadirannya rendah. Pemetaan faktor penyebab kerusakan zona penyangga berasal dari peningkatan pemukiman 30%, prilaku masyarakat, intensitas penangkapan dan illegal fishing

  10. Fishing Access Areas

    Data.gov (United States)

    Vermont Center for Geographic Information — The Vermont Fish & Wildlife Department maintains developed fishing access areas. These sites provide public access to waters in Vermont for shore fishing...

  11. Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization.

    Science.gov (United States)

    Silva, H C M; Martins-Júnior, M M C; Ribeiro, L B; Matoso, D A

    2017-03-30

    The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed.

  12. Kitab kuning dan perempuan, perempuan dan kitab kuning

    NARCIS (Netherlands)

    Bruinessen, M.M. van

    2007-01-01

    Kitab Kuning dan Emansipasi Perempuan: Konflik Budaya? Pengamatan-pengamatan Masdar mengenai kedudukan perempuan dalam diskursus (wacana, bahasan) dominan kitab kuning terasa tidak enak didengar tetapi memang sulit dibantah. Baik dalam penggunaan bahasa (yang sangat memihak kepada jenis mudzakkar) m

  13. Perancangan dan Analisis Redistribution Routing Protocol OSPF dan EIGRP

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2016-02-01

    Full Text Available Abstrak OSPF (Open Shortest Path First dan EIGRP (Enhanced Interior Gateway Routing Protocol adalah dua routing protokol yang banyak digunakan dalam jaringan komputer. Perbedaan karakteristik antar routing protokol menimbulkan masalah dalam pengiriman paket data. Teknik redistribution adalah solusi untuk melakukan komunikasi antar routing protokol. Dengan menggunakan software Cisco Packet Tracer 5.3 pada penelitian ini dibuat simulasi OSPF dan EIGRP yang dihubungkan oleh teknik redistribution, kemudian dibandingkan kualitasnya dengan single routing protokol EIGRP dan OSPF. Parameter pengujian dalam penelitian ini adalah nilai time delay dan trace route. Nilai trace route berdasarkan perhitungan langsung cost dan metric dibandingkan dengan hasil simulasi. Hasilnya dapat dilakukan proses redistribution OSPF dan EIGRP. Nilai delay redistribution lebih baik 1% dibanding OSPF dan 2-3% di bawah EIGRP tergantung kepadatan traffic. Dalam perhitungan trace route redistribution dilakukan 2 perhitungan, yaitu cost untuk area OSPF dan metric pada area EIGRP. Pengambilan jalur utama dan alternatif pengiriman paket berdasarkan nilai cost dan metric yang terkecil, hal ini terbukti berdasarkan perhitungan dan simulasi. Kata kunci: OSPF, EIGRP, Redistribution, Delay, Cost, Metric. Abstract OSPF (Open Shortest Path First and EIGRP (Enhanced Interior Gateway Routing Protocol are two routing protocols are widely used in computer networks. Differences between the characteristics of routing protocols pose a problem in the delivery of data packets. Redistribution technique is the solution for communication between routing protocols. By using the software Cisco Packet Tracer 5.3 in this study were made simulating OSPF and EIGRP redistribution linked by technique, then compared its quality with a single EIGRP and OSPF routing protocols. Testing parameters in this study is the value of the time delay and trace route. Value trace route based on direct calculation of cost

  14. BayFish: Bayesian inference of transcription dynamics from population snapshots of single-molecule RNA FISH in single cells

    National Research Council Canada - National Science Library

    Mariana Gomez-Schiavon; Liang-Fu Chen; Anne E West; Nicolas E Buchler

    2017-01-01

    Single-molecule RNA fluorescence in situ hybridization (smFISH) provides unparalleled resolution in the measurement of the abundance and localization of nascent and mature RNA transcripts in fixed, single cells...

  15. Perawatan Pergeseran Mandibula dan Kliking Menggunakan Teknik Edgewise dan Trainer

    Directory of Open Access Journals (Sweden)

    Rully Utami

    2014-06-01

    Full Text Available Kasus ini terjadi pada perempuan usia 22 tahun yang bersedia dipublikasikan untuk kepentingan  ilmu pengetahuan. Keluhan utama mandibula dan dagunya bergeser ke kanan, gigitan terbuka posterior dan bunyi click di persendian temporomandibular. Diagnosis pasien adalah maloklusi Angle kelas III tipe dento skeletal, pergeseran garis tengah mandibula dan dagu ke kanan, gigitan terbuka posterior dan clicking pada sendi temporomandibular. Perawatan dilakukan dengan teknik Edgewise dan trainer. Leveling dan unraveling dilakukan menggunakan kawat stainless steel bulat diameter 0,014 mm dengan multiloop. Trainer digunakan untuk koreksi pergeseran mandibula. Perawatan dilakukan selama 11 bulan, dan menunjukkan hasil hubungan molar pertama kanan menjadi kelas I. Overjet meningkat dari 0,1 mm menjadi 2 mm, overbite meningkat dari 0,2 mm menjadi 2,57 mm, garis tengah mandibula yang semula bergeser ke kanan 4,38 mm menjadi 2,53 mm, gigitan terbuka posterior dan clicking telah terkoreksi. Compromised Treatment of Class III Malocclusion with Mandibular Shifting, Posterior Openbite and Clicking Using Edgewise Technique and Trainer In Adult. This case report described the treatment of an adult female 22 years old who complained that her mandibula and chin shift to the right, posterior openbite and clicking. The patient diagnosed class III molar relationship, skeletal class III malocclusion, mandibular midline and chin shift to the right, posterior openbite and clicking on temporomandibular joint. Treatment was conducted using combination between  Edgewise Technique and trainer.  Leveling and unraveling are achieved by round stainless steel archwire 0,014 mm with multiloop. Trainer used to corrected the mandibular shifting. Result after 1 years treatment showed that the right molar relationship became class I, overjet increased  from 0,1 mm to 2 mm, overbite increased  from 0,2 mm to 2,57 mm, mandibular midline shifting decresed from 4,38 mm to 2,53 mm

  16. Tracking transparent monogenean parasites on fish from infection to maturity

    OpenAIRE

    Alejandro Trujillo-González; Constantinoiu, Constantin C.; Richard Rowe; Hutson, Kate S.

    2015-01-01

    The infection dynamics and distribution of the ectoparasitic fish monogenean Neobenedenia sp. (Monogenea: Capsalidae) throughout its development was examined on barramundi, Lates calcarifer (Bloch) (Latidae), by labelling transparent, ciliated larvae (oncomiracidia) with a fluorescent dye. Replicate fish were each exposed to approximately 50 fluorescent oncomiracidia and then examined for parasites using an epifluorescence stereomicroscope at 10 time intervals post-exposure (15, 30, 60, 120 m...

  17. Fluorescence-Based Sensors

    Science.gov (United States)

    Orellana, Guillermo

    The natural luminescent phenomena (from the Latin words "lumen" and "essentia", i.e., "made of light") such as northern lights (aurora borealis), marine brightness, glow-worms, shining putrid fish scales, "bluish"- appearing water when contained in certain wooden cups (quinine fluorescence), some stones heated at high temperatures with reducing agents (BaS phosphorescence), or light emitted while crushing sugar (triboluminescence) already fascinated our ancestors. Nowadays we understand that ultraviolet and visible emission of light originates from a competitive deactivation pathway of the lowest electronic excited state of atoms and molecules that produces the so called luminescence (the sub-terms fluorescence and phosphorescence just designate whether the return of the excited to the ground state is an "allowed" or "forbidden" process, namely it is fast or slow, the loosely-defined border between them being a 1-μs-1 rate constant). Actually, luminescence is the only method to generate light in the known Universe regardless it is powered by the nuclear reactions in the stars, the ohmical heating in bulbs, an electric discharge, the absorption of light or a (bio)chemical reaction (chemiluminescence).

  18. Do bacteria, not fish, produce 'fish kairomone'?

    NARCIS (Netherlands)

    Ringelberg, J.; Van Gool, E.

    1998-01-01

    Fish-associated chemicals enhance phototactic downward swimming in Daphnia. If perch were treated with the antibiotic ampicillin, this enhancement was significantly decreased. Therefore, not fish, but bacteria associated with fish, seem to produce this kairomone. [KEYWORDS: Diel vertical migration;

  19. KONSEP DAN PRAKTIK PENDIDIKAN MULTIKULTURAL DI AMERIKA SERIKAT DAN INDONESIA

    Directory of Open Access Journals (Sweden)

    Juju Masunah

    2011-11-01

    Full Text Available Abstract: Concepts and Practices of Multi Cultural Education in the United States and in Indonesia. The purpose of this article is to discuss multicultural education consepts and practices in the U.S.A and its application to Indonesia. Data is based on a case study research of two dance educators who implemented multicultural education concept in Columbus, Ohio in year 2007. The result of this research is that two dance educators teach students equally by developing a curriculum and pedagogy that face democracy for all students. This concept and practice is possible to be applied to dance education in Indonesia. Abstrak: Konsep dan Praktik Pendidikan Multikultural di Amerika Serikat dan Indonesia. Tujuan artikel ini adalah untuk mendiskusikan konsep dan praktik pendidikan multikultural di Amerika Serikat dan Indonesia. Data-data diperoleh dari hasil penelitian studi kasus dua orang pendidik tari di Columbus, Ohio yang menerapkan konsep pendidikan multikultural dalam pembelajaran tari pada tahun 2007. Peneli­tian ini menyimpulkan bahwa dua guru tari tersebut memberi perlakuan yang sama dan adil terhadap se­mua siswa dengan cara yang demokratis dengan memperhatikan keberagaman dan keperbedaan siswa. Cara-cara ini sangat memungkinkan untuk diaplikasikan dalam pendidikan tari di Indonesia.

  20. DISTRIBUSI, KELIMPAHAN DAN VARIASI UKURAN LARVA IKAN DI ESTUARIA SUNGAI MUSI

    Directory of Open Access Journals (Sweden)

    Eko Prianto

    2016-03-01

    Full Text Available Penelitian tentang distribusi, kelimpahan dan ukuran larva ikan dilakukan pada bulan Maret, Mei, Juni dan Oktober 2011. Stasiun pengambilan contoh meliputi Muara Delta Upang (stasiun 1, Muara Sungai Musi (stasiun 2 dan Pulau Payung (stasiun 3. Pengambilan larva pada siang hari menggunakan Bongo net yang berukuran mata jaring 250 µm. Hasil identifikasi diperoleh 13 famili ditinjau menurut musim, pada bulan Mei dan Oktober masing-masing diperoleh 7 famili, dan pada bulan juni sebanyak 3 famili. Kelimpahan larva ikan berkisar antara 9-46 ind/m3 dengan jumlah yang tertinggi (46 ind/m3 pada bulan Mei dan terendah pada bulan Juni (9 ind/m3. Larva ikan dari famili Gobiidae memiliki sebaran yang cukup luas baik spasial maupun temporal. Variasi ukuran larva ikan menurut famili setiap bulannya memiliki variasi ukuran yang hampir sama.  Research about the distribution, abundance and size of fish larvae was conducted in March, May, June and October 2011. Sampling stations encompasses Delta Upang (station 1, Muara Sungai Musi (station 2 and Pulau Payung (station 3. Larvae taken during the daytime using a Bongo net with mesh size of 250 µm. Identification results obtained 13 families based on the season, in May and October respectively 7 families, and in June as many as 3 families. Abundance of fish larval around 9-46 ind/m3 with the highest number (46 ind/m3 in May and the lowest in June (9 ind/m3. Larvae of Gobiidae family have a large distribution on spatial and temporal. The variation in size of fish larvae by family on each month are the same.

  1. RADIKALISME NEGARA DAN KEKUASAAN PERSPEKTIF POLITIK GLOBAL

    Directory of Open Access Journals (Sweden)

    M. Sidi Ritaudin

    2014-12-01

    Full Text Available Meskipun tragedi kemanusiaan yang berbasis terorisme bersifat domistik regional, tetapi sebab musababnya, setelah dilakukan penelusuran secara kritis dan tajam serta mendalam oleh para ahli, adalah dimensi politik global. Ini bukan asumsi tetapi fakta dan realita. Sebut saja misalnya tragedi WTC di Amerika serikat 11 September 2001, yang berbuntut pada pembumihangusan Irak, pembantaian dan pembunuhan secara keji di Palestina. Dan saat ini terjadi pembumihangusan Irak jilid dua dengan peristiwa ISIS, tragedi kemanusiaan di Ukraina dan Somalia. Sebahagian pemerhati Islam dan Timur Tengah menengarai bahwa motif gerakan radikalisme merupakan antitesa dari keserakahan politik, ekonomi, kekuasaan dan keangkuhan peradaban. Paradoksal radikalisme Islam dengan para aktivis pengusung ide sekularisme, pluralisme, dan liberalisme agama sudah berlangsung cukup lama dan tiada berkesudahan, menarik untuk didiskusikan.

  2. CORPORATE IDENTITY SEJARAH DAN APLIKASINYA

    Directory of Open Access Journals (Sweden)

    Christine Suharto Cenadi

    1999-01-01

    Full Text Available In the recent globalization era%2C and the developed market%2C many companies compete to attract consumers to buy their products. One of the keys to compete and survive in this developing market is by creating an image and graphic identity. This paper will discuss about corporate identity%2C image%2C its purpose and applications. Abstract in Bahasa Indonesia : Dalam era globalisasi dewasa ini%2C dan dengan berkembangnya pasar (market%2C banyak perusahaan bersaing untuk menarik perhatian konsumen untuk membeli produknya. Salah satu cara untuk bersaing dan dapat bertahan di dalam pasar yang terus berkembang ini adalah dengan menciptakan suatu image dan identitas graphic. Tulisan ini akan membahas tentang corporate identity%2C image%2C fungsi dan aplikasinya

  3. Memahami Patofisiologi dan Aspek Klinis Syok Hipovolemik: Update dan Penyegar

    Directory of Open Access Journals (Sweden)

    Hardisman Hardisman

    2013-09-01

    Full Text Available AbstrakSecara patofisiologis syok merupakan gangguan hemodinamik yang menyebabkan tidak adekuatnya hantaran oksigen dan perfusi jaringan. Gangguan hemodinamik tersebut dapat berupa penurunan tahanan vaskuler sitemik terutama di arteri, berkurangnya darah balik, penurunan pengisian ventrikel dan sangat kecilnya curah jantung. Gangguan faktor-faktor tersebut disbabkan oleh bermacam-macam proses baik primer pada sistim kardiovaskuler, neurologis ataupun imunologis. Diantara berbagai penyebab syok tersebut, penurunan hebat volume plasma intravaskuler merupakan faktor penyebab utama. Terjadinya penurunan hebat volume intravaskuler dapat terjadi akibat perdarahan atau dehidrasi berat, sehingga menyebabkan yang balik ke jantung berkurang dan curah jantungpun menurun. Penurunan hebat curah jantung menyebabkan hantaran oksigen dan perfusi jaringan tidak optimal dan akhirnya menyebabkan syok. Pada tahap awal dengan perdarahan kurang dari 10%, gejala klinis dapat belum terlihat karena adanya mekanisme kompensasi sisitim kardiovaskuler dan saraf otonom. Baru pada kehilangan darah mulai 15% gejala dan tanda klinis mulai terlihat berupa peningkatan frekuensi nafas, jantung atau nadi (takikardi, pengisian nadi yang lemah, penurunan tekanan nadi, kulit pucat dan dingin, pengisian kapiler yang lambat dan produksi urin berkurang. Perubahan tekanan darah sistolik lebih lambat terjadi akibat adanya mekanisme kompensasi tadi, sehingga pemeriksaan klinis yang seksama harus dilakukan.Kata kunci: syok, hipovolemik dan patofisiologiAbstractShock is hemodynamic disorders, which causes inadequate oxygen delivery and perfusion. The hemodynamic disorders can be decreasing of systemic vascular resistant, venous return, ventricular filling and inadequate of cardiac output. The disorders are caused by cardiovascular dysfunction, neurologic or immunologic factors. Intravascular volume loss is one of the main factors, which is caused by severe dehydration or bleeding. These

  4. Book Review: Hukum Laut Internasional dan Pengaturannya di Indonesia

    Directory of Open Access Journals (Sweden)

    Ayu Puji Lestari

    2016-08-01

    Full Text Available Buku ini ditulis oleh Prof. Dikdik Mohamad Sodik, S.H., M.H., Ph.D., dosen Fakultas Hukum Universitas Islam Bandung (UNISBA. Beliau merupakan pakar dalam bidang hukum laut internasional. Selain menjadi akademisi, Penulis juga aktif mengikuti training programme terkait hukum laut internasional yang diselenggarakan oleh International Ocean Institute Malta, dan Centre of Maritime Policy, Fakultas Hukum, Universitas Wollongong, New South Wales, Australia. Beliau juga aktif dalam menulis artikel hukum laut internasional mengenai masalah Illegal, Unreported and Unregulated (IUU Fishing pada 4 jurnal internasional. Beliau juga menjadi Guru Besar Utama di Fakultas Hukum UNISBA.

  5. POTENSI MAGGOT UNTUK PENINGKATAN PERTUMBUHAN DAN STATUS KESEHATAN IKAN

    Directory of Open Access Journals (Sweden)

    Melta Rini Fahmi

    2016-11-01

    Full Text Available Penggunaan maggot sebagai pakan alternatif ikan telah dikaji di Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot merupakan larva serangga black soldier (Hermetia illusence yang dapat mengkonversi material organik menjadi biomassanya. Salah satu keunggulan maggot adalah dapat diproduksi dalam berbagai ukuran, sesuai dengan kebutuhan. Penyimpanan maggot pada suhu rendah dapat menghambat pertumbuhan dan mempertahankan kehidupannya. Produksi maggot pada ukuran kecil dimulai dari penyediaan telur, penetasan, dan pembesaran dalam media PKM (Palm Kernel Meal atau bungkil kelapa sawit, pemanenan dan penyimpanan dalam suhu rendah. Nilai nutrisi maggot pada umur 6-7 hari adalah protein: 60,2%; lemak: 13,3%; abu: 7,7%; karbohidrat: 18,8%. Percobaan pemanfaatan maggot sebagai suplemen pakan diujikan terhadap ikan Balashark (Balantiocheilus melanopterus Bleeker ukuran 2,0 ± 0,2 g. Hasil yang didapatkan menunjukkan bahwa pemberian maggot memberikan pertumbuhan dan sintasan yang lebih baik. Dampak penambahan maggot pada ikan terlihat signifikan terhadap gambaran darah ikan yang menunjukkan daya tahan tubuh ikan yang lebih baik. Maggot utilization as fish feed alternative has been studied at Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot is an insect larvae of black soldier (Hermetia illusence that can convert organic material to its body biomass. One of the advantages in maggot culture is that it can be produced in different sizes according to fish requirement. Keeping maggot at low temperature can delay its growth while keeping it alive. Production of small size maggot starts from eggs preparation, hatching, and rearing in media of PKM (Palm Kernel Meal or coconut oil cake of palm, cropping and then keeping it in low temperature. Nutritional value of maggot at the age of 6-7 days is as follows: protein, 60.2%, fat; 13.3%, ash; 7.7%, carbohydrate; 18.8%. Trial feeding using maggot as feed supplement was done on Balashark

  6. Sifilis Laten: Diagnosis dan Pengobatan

    Directory of Open Access Journals (Sweden)

    Rasmia Rowawi

    2013-09-01

    Full Text Available Abstrak   Sifilis laten merupakan stadium sifilis yang diakibatkan oleh T. pallidum yang masih menetap dalam tubuh, namun tidak menunjukkan gejala dan hanya menunjukkan hasil pemeriksaan serologis yang reaktif. Sifilis laten yang tidak diterapi dapat menetap bertahun-tahun atau seumur hidup dan dapat meningkatkan risiko terinfeksi HIV. Ibu hamil dengan sifilis laten dini akan menyebabkan sekitar 40% bayi yang dilahirkankannya tertular, 20% prematur, 10% lahir mati, dan 4% meninggal pada waktu dilahirkan. Diagnosis sifilis laten dini ditegakkan bila dalam 12 bulan terakhir ditemukan satu atau lebih dari tanda-tanda berikut ini: peningkatan titer VDRL/RPR sebanyak empat kali atau lebih; pada anamnesis didapatkan gejala sifilis primer dan sekunder; riwayat kontak seksual dengan seseorang yang didiagnosis atau diduga menderita sifilis primer atau sifilis sekunder atau sifilis laten dini; serta kontak seksual dengan seseorang dengan tes VDRL atau RPR dan TPHA reaktif. Pengobatan yang direkomendasikan untuk sifilis laten dini adalah benzatin penisilin 2,4 juta UI, IM, dosis tunggal, sedangkan pada sifilis laten lanjut, benzatin penisilin 2,4 juta UI, IM, diberikan 3 kali dengan interval satu minggu. Pemeriksaan serologis sifilis non- treponemal (VDRL atau RPR dilakukan setelah pengobatan 3, 6, 12, dan 24 bulan untuk menilai keberhasilan pengobatan.   Kata kunci: Diagnosis, silifis laten, terapi

  7. SEJARAH PENGUASAAN SUMBER DAYA PESISIR DAN LAUT DI TELUK TOMINI

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    Muhammad Obie

    2015-01-01

    Full Text Available This article aims to analyze the historical milestones of coastal and sea resources management in Tomini Bay. It used a critical theory paradigm with two strategies, namely historical sociology and case studies. The collected data were primary and secondary ones, then were analyzed by using qualitative approach. The analysis results indicated that coastal and sea management in To-mini Bay could be divided into era before 1901, when Bajo Tribe was the sea adventurer in To-mini Bay as well as owning the resources. Since 1901 to independence era of Old Order, Bajo tribe began to settle to coastal area, built houses above the sea surfaces with economic resources coming from fishing and other sea pickings.  During the New Order, precisely from 1977 to Reformation Order, the existence of Bajo Tribe was terribly disturbed by the wood company, fishpond, and conservation policy. In this era, Bajo Tribe faced the resettlement pressure that caused their community was divided, Sea Bajo and Land Bajo.  This reality caused the access of the Land Bajo community to the coastal and sea resources was limited, while the Sea Bajo community was progressively under the pressure of of the expansion of the wood company, fishpond, and conservation policy.Key words: Bajo Tribe, wood company, fishpond, conservation, resettlement, cultural tourismTujuan penelitian ini adalah menganalisis tonggak-tonggak sejarah penguasaan sumber daya pesisir dan laut di Teluk Tomini. Penelitian ini menggunakan paradigma teori kritis, dengan strategi sosiologi sejarah dan studi kasus. Data yang terkumpul berupa data primer dan data sekunder, kemudian dianalisis dengan pendekatan kualitatif. Hasil penelitian menunjukkan bahwa penguasaan sumber daya pesisir dan laut di Teluk Tomini dapat dibagi atas masa sebelum tahun 1901, yang ditandai Suku Bajo sebagai pengembara laut di Teluk Tomini sekaligus me-nguasai sumber daya yang ada. Sejak tahun 1901 sampai masa kemerdekaan (Orde Lama, Suku Bajo

  8. Kondisi habitat dan keragaman nekton di hulu Daerah Aliran Sungai Wampu, Kabupaten Langkat, Provinsi Sumatera Utara

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    Ahmad Muhtadi

    2017-04-01

    Full Text Available The study was conducted to determine the condition of the habitat and the diversity of fish in the watershed of Wampu. The habitat conditions was determined by measuring parameters of temperature, brightness, current velocity, depth, DO, pH, type of substrate, the width and the width of the river. Nekton was sampled using electrofishing units at voltage of 15 volt and 9 amperes. The characteristic of upstream watershed was large stones substrate, gravel, and sand with a fast flow and clear waters. Physical-chemical parameters of waters in the upper watersheds Wampu is still suitable for fish, crabs, and shrimp to live in good condition. A total of 15 species of fishes and one species of freshwater crab and one species of freshwater prawns were recorded during the study. There were two species of mahseer were recorded, namely; Tor soro and T. tambra. The higher diversity index (H ' was recorded in the Bahorok River with a value of 4.5 followed by Berkail River (3.45 and Landak River with a value of 2.46. Penelitian ini dilakukan untuk mengetahui kondisi habitat dan keragaman ikan di DAS Wampu. Pengukuran kondisi habitat dengan mengukur parameter suhu, kecerahan, kecepatan arus, kedalaman, DO, pH, jenis substrat, lebar dan lebar badan sungai. Jenis nekton ditangkap dengan alat backpack electrofishing units dimana arus listrik yang dihasilkan bersumber dari batere 15 volt dan 9 ampere. Kondisi habitat pada bagian hulu DAS  Wampu yaitu bertipe substrat batu besar, kerikil, dan pasir dengan arus yang sedang - besar serta perairan yang jernih. Parameter fisika-kimia perairan di hulu DAS Wampu masih layak dan cocok untuk habitat ikan, kepiting dan udang. Selama penelitian nekton yang terkoleksi sebanyak 15 jenis ikan dan 1 jenis kepiting air tawar serta 1 jenis udang air tawar. Pada survei ini ditemukan dua jenis ikan Tor  yakni  T. soro, dan T. Tambra. Keanekaragaman (H' tertinggi terdapat pada Sungai Bahorok dengan nilai 4,5 diikuti Sungai Berkail

  9. 膀胱移行细胞癌各分期分级中荧光原位杂交检测敏感性比较%Evaluation on significance of fluorescence in situ hybridization (FISH) for detection of bladder cancer

    Institute of Scientific and Technical Information of China (English)

    徐秀红; 杜雨; 张道新; 田野

    2011-01-01

    Objective In order to look for a better non - invasive method for diagnosing bladder cancer, the fluorescence in situ hybridization ( FISH ) technique had been applied in diagnosis of bladder cancer. Methods FISH and cytological analysis were performed simultaneously on urine - exfoliated cells from 138 patients with hematuria. Chromosomal abnormalities and gene mutation were determined by FISH with probes for chromosomes 3,7, 17 and pl6. The sensitivity of FISH and cytological examination in diagnosing transitional cell carcinoma of bladder was studied and compared. Results Big and irregular cell nuclei and position effect variegation were seen in some cells ( P < 0. 05 ). In stage Tl bladder cancer, the sensitivity of FISH and cytological analysis was 83 . 6 % and 31.3% respectively, while in stage T2 - T4 , their sensitivity was 100 % and 55. 6 % respectively. Their sensitivity was 36. 3 % and 9.1% respectively with grade Gl versus 95. 2 % and 42. 9 % respectively with grade G2, and the sensitivity was 100 % and 50 % respectively with grade G3 ( P < 0. 05 ) in a comparable manner. There was significant difference in total sensitivity for diagnosis and staging of transitional cell carcinoma of bladder between FISH and cytological examination ( P < 0. 05 ). Conclusion FISH is more sensitive than cytological examination, hence it may be applied as a valuable method for diagnosing transitional cell carcinoma of bladder.%目的 评价荧光原位杂交(FISH)在诊断膀胱移行细胞癌各分期分级中的敏感性.方法 应用荧光标记的3、7、17号染色体及p16基因探针检测138例血尿患者尿液中脱落细胞的染色体及基因畸变情况,根据诊断标准计算FISH诊断膀胱移行细胞癌的敏感性.同时留取上述患者尿液送病理科行尿找瘤细胞检查,并比较两种方法对膀胱移行细胞癌的诊断效能.结果 FISH图像中细胞核大且不规则者更易观察到染色体基因异常情况,并可见具有较多染

  10. The Fishing Cat

    Institute of Scientific and Technical Information of China (English)

    孙雅飞; 乐伟国

    2008-01-01

    @@ 一、故事内容 A cat goes fishing every day. He wants to eat fish, but he can't catch any fish. One day, he goes to the river as usual. Suddenly, a fish comes out. He catches the fish and putsthe fish in the basket. He's very happy, but he forgest to put the lid on the basket.

  11. Sistem Komunikasi dan Informasi di Indonesia

    Directory of Open Access Journals (Sweden)

    Atie Rachmiatie

    2000-12-01

    Full Text Available Suatu Perubahan dan Tantangan Masa Depan Terdapat perubahan pola komunikasi dan informasi pada masyarakat. terutama perubahan dari segi perangkat keras, perangkat lunak. dan sumber daya manusia, yang berpengaruh terhadap sistem komunikasi dan informasi nasional, baik yang terjadi pada individu maupunpada lembaga/institusi yang berkaitan. Perubahan menyangkut pulapada kondisi organisasi pemerintah yang terkait dengan pengelolaan informasi. yaitu dengan ditata kembalinya sistem administrasi dan manajemen, baik secara intern, maupun ekstern berkaitan dengan organisasi luar. Apresiasi masyarakat terhadap materi informasi. pada kenyataannya. belum tersosialisasikan secara optimal. karena hal ini tergantung pada kebutuhan dan prioritas kebijakan institusi pemerintahan setempat. Dengan demikian. tidak semuajenis isi/materi informasi mendapatkan porsi yang sama dalam pemasyarakatannya.

  12. Diffusion des macromolécules de dextrane dans le gel Séphadex G-200

    Science.gov (United States)

    Moussaoui, M.; Hamieh, T.; Toufaily, J.; Naoufal, D.

    2005-05-01

    Dans cet article nous avons étudié la diffusion des macromolécules de dextrane marquées à l'isothiocyanate de fluorescéine (FITC), dans les perles de l'ultrogel AcA34, par la méthode du retour de la fluorescence après photoblanchiment (FRAP). Le coefficient de partage chromatographique (Kc), a été déterminé par chromatographie sur une colonne de l'ultrogel AcA34. Le coefficient de partage microfluorimétrique (Kf) a été déterminé par la technique de (FRAP), en mesurant à la fois, la fluorescence dans la perle et dans le solvant. La relation entre le coefficient de partage chromatographique et le rayon de Stokes (Rs) des macromolécules a été étudiée en appliquant la relation de Laurent et Killender, alors que la variation du coefficient de diffusion relatif D/D0, a été étudiée en fonction de Kc en appliquant la théorie d'Ogston et al.

  13. KAJIAN BIOAKTIF DAN ZAT GIZI PROPOLIS INDONESIA DAN BRASIL

    Directory of Open Access Journals (Sweden)

    Eliza Halim

    2012-11-01

    Full Text Available ABSTRACTIndonesia has a potency to produce its own propolis, however the propolis market in Indonesia is dominated by imported product, such as from Brazil. Currently, still there is no reasearch which evaluate bioactive compound and nutrient content of Indonesian Propolis (IP compare with Brazilian Propolis (BP. The objectivesof this study were to analyze bioactive compounds and nutrient contents of IP compared to BP. Bioactive compounds and nutrients content were analyzed by gas chromatography–mass spectrophotometry. The resultsshowed both IP and BP contain fenol, α-amyrin, cylolanost, and pyrimidines. Bioactive compounds which specifically found in IP were eudesmane compound, ethyl acridine, lupeol, friedooleanan; while β amyrin and cinnamic acid compound only found in BP. The nutrient contents of IP were higher than BP except for vitamin A. In conclusion, IP might have potential health benefit, similar to BP.Key words: propolis, bioactive, nutrientABSTRAKIndonesia mempunyai potensi untuk menghasilkan propolis tetapi pemasaran propolis di Indonesia didominasi oleh propolis impor seperti propolis yang berasal Brasil. Sampai saat ini belum ada penelitian yang mengungkapkandungan bioaktif dan zat gizi propolis Indonesia (PI dibandingkan dengan propolis Brasil (PB. Oleh karena itu tujuan penelitian ini adalah untuk melakukan kajian kandungan bioaktif dan zat gizi (vitamin dan mineral PI dibandingkan dengan PB. Komponen bioaktif dan kandungn gizi dianalisis dengan metode gas chromatography–mass spectrometry. Hasil analisis menyatakan bahwa baik PI maupun PB mengandung senyawa fenol, α-amyrin, cylolanost, dan pirimidin. Komponen bioaktif unik yang ditemukan di dalam PI adalah senyawaeudesmane, ethyl acridine, lupeol dan friedooleanan; sedangkan β-amyrin dan senyawa asam sinamat hanya ditemukan di dalam PB. Kandungan zat gizi PI lebih tinggi dari PB kecuali kandungan vitamin A. Hal ini menunjukkan bahwa PI kemungkinan mempunyai khasiat untuk

  14. Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections

    DEFF Research Database (Denmark)

    Jensen, Henrik Elvang; Jensen, Louise Kruse; Barington, Kristiane

    2015-01-01

    sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue......Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target...

  15. Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections

    DEFF Research Database (Denmark)

    Elvang Jensen, Henrik; Jensen, Louise Kruse; Barington, Kristiane

    2015-01-01

    sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue......Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target...

  16. OPTIMALISASI ZONA PEMANFAATAN WISATA TAMAN NASIONAL KARIMUNJAWA MELALUI KOMUNITAS IKAN KARANG (Optimizing The Tourism Utilization Zone Karimunjawa National Park through Coral Reef Fish Communities

    Directory of Open Access Journals (Sweden)

    Rohmani Sulisyati

    2016-07-01

    . Diving pemula dilakukan pada pulau-pulau dengan resiko penyelaman rendah dan sedikit famili, sementara untuk advanced dilakukan pada pulau-pulau dengan sumberdaya tinggi dan beragam famili ikan.  ABSTRACT Ecotourism was closely related to environmental conservation that could be mutually supported and valuated. Conservation related with the communities condition. The tourism utilization zone required the coral reef fish communities as a tourist attraction. The aim of the study was optimized the tourist utilization zone Karimunjawa National Park for tourism activities through coral reef fish communities. Coral reef fish data collected on a belt transect by a visual census, record families and the number of fishes are found and the approximate total length of fish. The research conducted  during November 2013 at 14 locations.  The transect was deployed at two depth variations i.e 3 and 6–8 meters to represent the shallow and the deep water. Quantitative analysis was done to measure the fish abundance and diversity index, evenness index and domination. The result showed in the shallow water found 18 coral fish families. Chaetodontidae, Labridae and Scaridae can be found at all locations. Fish abundance is moderate which a condition of fish community depressed to stable. While the deep water, there were 17 families. Caesionidae, Chaetodontidae, Labridae, and Scaridae can found in all locations. Fish abundance is moderate which a condition of fish community depressed to stable. In the shallow waters of the outer west island inhabited by a group of major fish with a little family; the center of the archipelago encountered diverse fish families and the outer islands west and east there is a dominance of fish. The islands were located in the middle of the islands suitable for snorkeling. In the deep waters were divided four groups of islands i.e. islands with fair coral cover and the few families of coral reef fish, islands which had a fair coral cover with abundant fish

  17. PERBANDINGAN KARAKTERISTIK KIMIA KOPI LUWAK DAN KOPI BIASA DARI JENIS KOPI ARABIKA (Cafeea arabica. L) DAN ROBUSTA (Cafeea canephora. L)

    OpenAIRE

    Israyanti, -

    2012-01-01

    Penelitian ini bertujuan untuk mengetahui perbandingan jumlah kafein, karakteristik proksimat (protein dan lemak) dan rasa dan aroma antara kopi luwak dan kopi biasa dari jenis arabika (Cafeea arabica. L) dan robusta (Cafeea canephora. L). Perlakuan penelitian yakni A1 (luwak robusta), A2 (luwak arabika), B1 (robusta biasa) dan B2 (arabika biasa). Parameter penelitian yaitu pengujian organoleptik pada rasa (metode hedonik) dan aroma (metode ranking), analisa kafein dan uji proksimat berupa ...

  18. Fish gelatin.

    Science.gov (United States)

    Boran, Gokhan; Regenstein, Joe M

    2010-01-01

    Gelatin is a multifunctional ingredient used in foods, pharmaceuticals, cosmetics, and photographic films as a gelling agent, stabilizer, thickener, emulsifier, and film former. As a thermoreversible hydrocolloid with a narrower gap between its melting and gelling temperatures, both of which are below human body temperature, gelatin provides unique advantages over carbohydrate-based gelling agents. Gelatin is mostly produced from pig skin, and cattle hides and bones. Some alternative raw materials have recently gained attention from both researchers and the industry not just because they overcome religious concerns shared by Jews and Muslims but also because they provide, in some cases, technological advantages over mammalian gelatins. Fish skins from a number of fish species are among the other sources that have been comprehensively studied as sources for gelatin production. Fish skins have a significant potential for the production of high-quality gelatin with different melting and gelling temperatures over a much wider range than mammalian gelatins, yet still have a sufficiently high gel strength and viscosity. Gelatin quality is industrially determined by gel strength, viscosity, melting or gelling temperatures, the water content, and microbiological safety. For gelatin manufacturers, yield from a particular raw material is also important. Recent experimental studies have shown that these quality parameters vary greatly depending on the biochemical characteristics of the raw materials, the manufacturing processes applied, and the experimental settings used for quality control tests. In this review, the gelatin quality achieved from different fish species is reviewed along with the experimental procedures used to determine gelatin quality. In addition, the chemical structure of collagen and gelatin, the collagen-gelatin conversion, the gelation process, and the gelatin market are discussed.

  19. PERANAN SEKTOR PERIKANAN DALAM PEMBANGUNAN WILAYAH DAN STRATEGI PENGEMBANGANYA DALAM RANGKA OTONOMI DAERAH KABUPATEN BALANGAN

    Directory of Open Access Journals (Sweden)

    Syahrida Ariani

    2016-06-01

    , Perdagangan dan Koperasi memfasilitasi peta jalur pemasaran bagi petani ikan sehingga harga ikan bisa tetap stabil, 9 Perlu melakukan pembinaan dan pendampingan kepada masyarakat terutama untuk diversifikasi poduk menjadi produk olahan, 10 Perbaikan fasilitas infrastruktur dan fungsi sarana dan prasarana produksi budidaya perikanan melalui perbaikan saluran irigasi dan BBI, 11 Pemerintah daerah lebih mensosialisasikan skim perkreditan untuk rakyat KUR melalui sosialisasi kepada masyarakat. Balangan Regency has a fairly large waters resources and potentially increase revenues original areas, expected role of Government that has the authority Balangan Regency as Executive exploration and wealth management waters should be able to dig into the potential of the fishery to be more optimal in order to exercise the autonomy of the region.  In General, this research aims to apply the techniques of planning regional development Balangan Regency. Whereas in particular this research aims to know the role of the fisheries sector to the economy and setting the Balangan Regency alternative strategies for the development of fisheries sector in the framework of regional autonomy in Balangan Regency. Research methods used are descriptive qualitative, and used case studies approaches and analysis of the data used  descriptive analysis, analysis of profit and SWOT analysis (Strategic Issues and problem identification. Based on the results of the SWOT analysis and the calculation of weighting matrix with QSP obtained the alternative order of precedence for the development strategy of the fishery as follows: 1 local government commitment to the development of the fisheries sector through the allocation of the budget, 2 of local government through the Office of PTPHPP improves  the role of BBI and UPR to produce quality seeds and stem and have a certification, 3 increases the role and function of fisheries extension, officers, 4 increase the utilization potential of land fish breeding with

  20. Progress in molecular diagnosis of Charcot-Marie-Tooth-disease type 1 (CMT 1, HMSN I) and hereditary neuropathy with liability to pressure palsies (HNPP) by fluorescence in situ hybridization (FISH)-detection of a potential genetic mosaicism

    Energy Technology Data Exchange (ETDEWEB)

    Bathke, K.; Liehr. T.; Ekici, A. [Institute for Human Genetics, Erlange (Germany)] [and others

    1994-09-01

    We tested 20 CMT 1 patients characterized according to the criteria of the European CMT consortium by Southern hybridization of MspI restricted genomic DNA with probes pVAW409R1, pVAW412Hec and pEW401HE. In 11 of the 20 CMT 1 cases (55%), we observed a duplication in 17q11.2; one patient had a dinucleotide insertion in exon 6 of the PO-gene (5%). One HNPP case had a typical 17p11.2 deletion. Analysis of CA-repeats was performed with primers RM11GT and Mfd41; SSCP-analysis of the PO, PMP22 and Cx32-genes is in progress. FISH was carried out with probe pVAW409R1. 125 interphase nuclei were analyzed for each proband by counting the signals per nucleus. Normal cells show a characteristic distribution of signals: 1 signal in 5.9% of nuclei, 2 in 86.3% and 3 in 7.8%. A duplication is indicated by a shift to 3 signals in more than approximately 60% and 2 in less than 25% of the nuclei. In contrast, the 17p11.2 deletion of the HNPP patient shifts to 82.4% of nuclei with a single hybridization signal versus 14.4% with 2 signals. We detected one case with significantly abnormal distribution of interphase nuclei hybridization signals compared to cultures of normal cells and to those with 17p11.2 duplication or deletion: 3.2% nuclei revealed 1 signal, 48.0% two signals and 48.8% 3 signals, indicating a pathogenic but moderate dosis increase compared to the throughout duplicated cases. FISH with probe pVAW409R1 is a versatile tool to detect the HNPP deletion both in interphase nuclei and in metaphase chromosomes. In CMT 1 disease interphase nuclei are required for FISH analysis due to the small duplication of 1.5 Mbp. In contrast to Southern techniques, FISH is able to detect genetic mosaicism.

  1. Fish hemoglobins

    Directory of Open Access Journals (Sweden)

    P.C. de Souza

    2007-06-01

    Full Text Available Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemical strategies to adapt to the changing environmental gas availability. Structurally, most fish hemoglobins are tetrameric; however, those from some species such as lamprey and hagfish dissociate, being monomeric when oxygenated and oligomeric when deoxygenated. Fish blood frequently possesses several hemoglobins; the primary origin of this finding lies in the polymorphism that occurs in the globin loci, an aspect that may occasionally confer advantages to its carriers or even be a harmless evolutionary remnant. On the other hand, the functional properties exhibit different behaviors, ranging from a total absence of responses to allosteric regulation to drastic ones, such as the Root effect.

  2. PERANAN KOMPETENSI INTI DALAM MERGER DAN AKUISISI

    Directory of Open Access Journals (Sweden)

    Dwi Sulistiani

    2013-11-01

    Full Text Available Dalam era globalisasi dan pedagangan  bebas dewasa ini strategi merger dan akuisisi  merupakan fenomena yang semakin marak dilakukan oleh para pelaku bisnis dan  semakin menarik untuk dicermati bersama. Untuk menjalankan perusahaan para manajemen perusahaan selalu dituntut menjaga pertumbuhan usahanya secara optimal. Penerapan strategi merger dan akuisisi merupakan jalan pintas untuk mewujudkan pertumbuhan usaha tanpa perusahaan perlu memulai dari awal suatu jenis usaha yang baru. Dalam suatu kondisi perusahaan yang kuat cenderung membeli perusahaan lain untuk meningkatkan daya saing dan penghematan biaya. Perusahaan yang terlibat dalam merger dan akuisisi akan dapat diperoleh pangsa pasar yang lebih besar dan efisiensi terhadap biaya. Perusahaan yang merasa tidak dapat bertahan hidup secara individual biasanya merelakan diri untuk menjadi target akuisisi. Merger dan akuisisi merupakan suatu realitas dan tantangan yang harus siap dihadapi oleh para pelaku usaha dalam era perdagangan bebas saat ini. Namun tingkat kesuksesan dalam melakukan kegiatan merger dan akuisisi juga sangat dipengaruhi oleh kondisi internal perusahaan meliputi sumber daya, kapabilitas dan kompetensi inti yang dimiliki oleh perusahaan. Tulisan ini akan mencoba memberikan pemahaman bahwa sumber daya, kapabilitas dan  kompetensi inti merupakan faktor penting dalam melakukan kegiatan penggabungan usaha yang pada akhirnya dapat menentukan tingkat keberhasilan proses merger dan akuisisi yang sedang dijalankan oleh perusahaan

  3. RANCANG BANGUN MODEL KOMPRESI DAN TARIK PERMODELAN SANDBOX DAN MANFAATNYA

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    Fahrudin Fahrudin

    2015-07-01

    Full Text Available Permodelan sandbox dibuat dengan system tektonik konvergen dan divergen dengan menggunakan pergerakan satu sumbu. Permodelan ini bertujuan untuk membuat alat mesin sandbox yang bisa digunakan untuk penelitian dan pengajaran. Mesin berhasil dibuat dan sudah diujicobakan. Ujicoba dengan menggunakan satu lapisan pasir yang diambil dari Formasi Ngrayong. Percobaan selanjutnya dengan beberapa lapisan. Percobaan dilakukan dengan pengamatan yang meliputi pengamatan permukaan dan penampang verikal. Pengamatan yang aspek morfologi, kelurusan struktur, dan perkembangan sesar yang terbentuk. Hasil percobaan menunjukkan bahwa pembentukan morfologi sangat berkaitan dengan pembentukan sesar. Struktur sesar dengan pola forward propagating thrust faults. Sesar tersebut disebabkan imbrikasi. Lipatan yang terbentuk akibat mekanisme propagasi sesar.[Design of Compression and Extensional of the Sandbox Model and Its Benefit] Divergent and convergent tectonic sytem can be studied from the sandbox modelling. This model has a axis of movement. Sandbox models itends to study the progress of structural geology such as fault and fold. We successfully made machine of sandbox. This machine has basal detachment from duraluminum. Material for model is taken by loose sand from Ngrayong Formation. This experiment focused to observation about morphology in surface and thrust or backthrust in subsurface. This experiment has compression system. Result of model was that morphological sequences associated with fault sequences. Fault is formed to have the pattern of forward propagating thrust faults. It’s caused by imbricate thrust system. Folding is formed by the mechanism of fault propagation folding. 

  4. MUWUJUDKAN BUDAYA POLITIK SANTUN, BERSIH DAN BERETIKA DALAM RANGKA MEMPERKOKOH KEHIDUPAN BERBANGSA DAN BERNEGARA

    Directory of Open Access Journals (Sweden)

    Wahyu Widodo

    2014-11-01

    Full Text Available Abstract Makalah ini membahas konsep budaya politik berpusat pada imajinasi (pikiran dan perasaan manusia yang merupakan dasar semua tindakan. Dalam rangka menuju arah pembangunan dan modernisasi suatu masyarakat akan menempuh jalan yang berbeda antara satu masyarakat dengan yang lain, dan itu terjadi karena peranan kebudayaan sebagai salah satu faktor. Budaya politik dapat membentuk aspirasi, harapan, preferensi, dan prioritas tertentu dalam menghadapi tantangan yang ditimbulkan oleh perubahan sosial politik. Pada gilirannya, disimpulkan bahwa peran budaya politik santun, bersih dan beretika dalam rangka memperkokoh kehidupan berbangsa dan bernegara menuju Indonesia baru adalah: pertama, etika politik dan pemerintahan mengandung misi kepada setiap pejabat dan elite politik untuk bersikap jujur, amanah, sportif, siap melayani, berjiwa besar, memiliki keteladanan, rendah hati, dan siap untuk mundur dari jabatan publik apabila terbukti melakukan kesalahan dan secara moral kebijakannya bertentangan dengan hukum dan rasa keadilan masyarakat. Kedua, perlu dilakukan upaya penanaman suatu kesadaran bahwa politik yang hendak kita perjuangkan bukan semata politik kekuasaan, melainkan suatu politik yang mengedepankan panggilan pengabdian demi kesejahteraan masyarakat luas, dialektika antara partai dan politikus serta masyarakat yang kritis. Ketiga, budaya politik santun, bersih dan beretika ini diperlukan karena dapat membuat para elite politik menjauhi sikap dan perbuatan yang dapat merugikan bangsa Indonesia. Akhirnya, disarankan agar dilaksanakan kembali pendidikan budi pekerti yang merupakan pondasi bagi pelaksanaan Civic Education agar tercipta generasi yang tidak hanya mau menjadi politisi, namun paham budaya dan etika politik.   Kata kunci: budaya politik, kehidupan berbangsa dan bernegara, menuju Indonesia baru

  5. UJI TOKSISITAS DAN PERUBAHAN STRUKTUR MIKROANATOMI INSANG IKAN NILA LARASATI (Oreochromis nilloticus YANG DIPAPAR TIMBAL ASETAT

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    FAM Mulyani

    2014-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui Nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati yang dipapar timbal; mengetahui perubahan struktur  mikroanatomi dan mengetahui pada konsentrasi berapa timbal menyebabkan perubahan struktur mikroanatomi insang. Sampel uji toksisitas sebanyak 120 ekor untuk mengetahui tingkat  kematian ikan hingga 50% dalam 96 jam, dan uji perlakuan sebanyak 80 ekor dengan konsentrasi 0 ppm; 259,51 ppm; 291,94 ppm; dan 324,38 ppm. Pada akhir minggu ke empat diambil sampel insang dan dilakukan analisis gambaran struktur mikroanatomi insang secara diskriptif. Hasil analisis menunjukkan kerusakan edema 0%-25%, fusi lamela antara 1%-75%, hiperplasia 0%-50%, epithelial lifting 0%-50%, dan nekrosis 0%-50%. Dari hasil penelitian dapat disimpulkan bahwa nilai  LC50-96 jam pada uji toksisitas ikan Nila Larasati sebesar 324,38 ppm dan mulai terjadi perubahan struktur mikroanatomi insang pada konsentrasi 259,51 ppm. The objective of thje study was to determine the value of LC50-96 hours in a toxicity test of Nila Larasati fish exposed to lead acetate; to determine the changes in the microanatomy structure and to determine the concentration of lead acetate which causes microanatomy structure changes in the gills of Nila Larasati fish. The samples were 120 fish to find out the 50% fish mortality within 96 hours, and 80 fish in treatment test with the lead acetate concentrations of 0 ppm; 259.51 ppm; 291.94 ppm; and 324.38 ppm. At the end of the 4th week of the research, the gill samples were taken and the analysis of the microanatomy structure was carried out descriptively. The result of the analysis showed that there were 0%-25% edema, 1%-75% lamella fusion, 0%-50% hyperplasia, 0%-50% epithelial lifting and 0%-50% necrosis. It was concluded that the value of LC50-96 hours in Nila Larasati fish toxicity test was 324.38 ppm; and microanatomy changes started to occur in the lead acetate concentration of 259.51 ppm. 

  6. PENGARUH FLUKTUASI TINGGI MUKA AIR TERHADAP HASIL TANGKAPAN IKAN DI SUNGAI DAN RAWA MAHAKAM HULU KALIMANTAN TIMUR

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    Kamaluddin Kasim

    2015-12-01

    Full Text Available Beberapa penelitian menyebutkan bahwa fluktuasi tinggi muka air (TMA dapat mempengaruhi hasil tangkapan ikan di perairan sungai dan rawa namun tidak terhadap semua jenis ikan.  Penelitian ini dilakukan dengan tujuan untuk mengetahui hasil tangkapan jenis ikan sungai dan rawa sungai Mahakam yang mendapat pengaruh fluktuasi TMA dan jenis ikan yang tidak mendapatkan pengaruh langsung oleh fluktuasi TMA. Data mengenai hasil tangkapan ikan yang berasal dari alat tangkap pancing dan jaring diperoleh melalui enumerator di Tempat Pendaratan Ikan (TPI Selili Kota Samarinda pada periode 2007-2012, sedangkan nilai rata-rata Tinggi Muka Air (TMA DAS Mahakam secara bulanan diperlukan sebagai salah satu faktor yang diduga berpengaruh terhadap hasil tangkapan beberapa jenis ikan sungai dan rawa. Data dianalisis dengan metode regresi linear sederhana dan penentuan perbedaan hasil tangkapan pada musim hujan, peralihan dan kemarau dilakukan dengan Analysis of Variance (ANOVA. Hasil penelitian menunjukkan bahwa jumlah hasil tangkapan ikan berkorelasi kuat (r=0,7 terhadap Tinggi Muka air (TMA dengan arah hubungan negatif atau berkebalikan, yakni semakin tinggi nilai TMA maka hasil tangkapan semakin rendah. Jenis ikan sungai dan rawa seperti patin, nila, sepat siam (Trichogaster pectoralis, lais dan betok (Anabas testudineus merupakan jenis ikan yang hasil tangkapannya dipengaruhi secara signifikan (P0,05 oleh fluktuasi TMA.   Some studies have showed that water level fluctuation may have a significant correlation to the catch of several commercial fish target in inland fishery and does not influence directly the cath of some commercial fish. This study aimed to determine which species are directly influenced and such species not inluenced by water level fluctuation for its catches. Catch data obtained from hand line and gill net are recorded by enumerators at Fish Landing Sites of Selili, Samarinda during the period of 2007-2012, while the data of surface water

  7. 转基因水稻中外源基因的荧光原位杂交(FISH)分析%DETECTION AND ANALYSIS OF ALIEN GENES IN TRANSGENIC RICE BY FLUORESCENCE IN SITU HYBRIDIZATION

    Institute of Scientific and Technical Information of China (English)

    金危危; 李霞; 李宗芸; 宁顺斌; 凌定厚; 宋运淳

    2001-01-01

    利用荧光原位杂交(FISH),在供试8个转基因水稻株系的染色体上检出了转入的外源基因barnase-ps1片段.其中两株系各检出了两个不同的信号位点,而其他株系均只检出一个信号位点.所有染色体着丝粒区都没有杂交信号.结合Southern杂交及表达分析发现,大多数株系中,整合在染色体端部区的barnase基因表现为高水平表达,而靠近着丝粒区的则表现为低水平表达,表明表达水平与转基因整合位置可能存在一定程度的相关性.表达水平与拷贝数无明显相关.本文还利用多色荧光原位杂交(Multi-color FISH)技术,进行了barnaseps1片段与报道基因pHctinG的共杂交,多数情况下,barnase-ps1片段与报道基因整合在染色体的同一位置.

  8. FISH and FICTION to detect chromosomal aberrations in lymphomas.

    Science.gov (United States)

    Giefing, Maciej; Siebert, Reiner

    2013-01-01

    Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique allowing the visualization of target sequences like genes in interphase nuclei. It is widely used in routine diagnostics to identify cancer specific aberrations including lymphoma associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping-a technique called Fluorescence Immunophenotyping and Interphase Cytogenetic as a Tool for Investigation Of Neoplasia (FICTION)-it is moreover possible to identify a cell population of interest. Here we describe standard protocols for FISH and FICTION as used in our laboratory in diagnosis and research.

  9. PENGATURAN SUBSIDI PERIKANAN DALAM WTO DAN DAMPAKNYA BAGI INDONESIA

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    Hilton T. Putra

    2015-02-01

    Full Text Available Fisheries subsidies have been practiced by many states in the world. Such actions are considered as one of the causes of over-fishing. Its also endanger sustainable fisheries management. There is no specific regulation on fisheries subsidies in the WTO. To date, negotiation over fisheries subsidies came out with the draft and it would be attach as Annexes VIII of SCM Agreement. The draft of fisheries subsidies specifically regulates prohibited subsidies, general exception and special and different treatment. The impact of the WTO fisheries subsidies draft will be negative to the Indonesian fisheries market, fishery resources and employment.   Subsidi perikanan telah dipraktikkan oleh negara-negara di dunia. Tindakan tersebut dianggap sebagai salah satu penyebab terjadinya eksploitasi sumber daya perikanan sehingga membahayakan pengelolaan perikanan berkelanjutan. Terkait dengan perdagangan dan subsidi perikanan, belum ada regulasi khusus tentang subsidi perikanan di WTO. Negosiasi di WTO hingga saat ini baru pada rancangan yang akan menjadi lampiran VIII SCM Agreement. Rancangan subsidi perikanan di WTO mengatur secara khusus tentang subsidi perikanan yang dilarang dan pengecualian secara umum. Dampak dari rancangan subsidi perikanan WTO tersebut apabila berhasil disahkan akan memberikan dampak negatif kepada Indonesia terkait dengan pasar perikanan, sumber daya perikanan dan lapangan pekerjaan.

  10. KEMAMPUAN GENTENG PLASTIK BERGELOMBANG (CORRUGATED PLASTIC SEBAGAI BIOFILTER PARTIKEL AMONIAK DAN BAHAN ORGANIK DI MEDIA BUDIDAYA DAN LIMBAH CAIR BUDIDAYA IKAN (Performance of Corrugated Plastic as Biofilter of Ammonia Particle and Organic Material

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    Muslim Muslim

    2010-07-01

    Full Text Available ABSTRAK Pertumbuhan budidaya ikan dalam beberapa dekade ini berkembang sangat pesat, hal ini karena permintaan akan ikan meningkat. Meningkatnya kegiatan budidaya ikan selalu diiringi dengan meningkatnya limbah yang dihasilkan. Hal ini akan sangat cepat berpengaruh bila sistem budidaya yang dipakai adalah semi intesif atau intensif. Limbah tersebut harus segera dihilangkan atau dikurangi, karena akan berdampak pada ikan yang dibudidaya dan lingkungan seperti sungai dan laut. Tujuan penelitian ini adalah ingin mengetahui kemampuan genteng plastik bergelombang mengurangi limbah yang dihasilkan budidaya ikan yaitu Total Suspended Sediment (TSS, Suspended Sediment (SS, amoniak dan bahan organik (COD. Dari hasil penelitian diperoleh bahwa air limbah budidaya ikan yang mengandung TSS, SS, amoniak dan bahan organik setelah dilewatkan dengan genteng plastik bergelombang konsentrasinya menurun dengan tingkat efisiensi pengurangan yang terjadi di dalam kolam ikan dan di luar kolam ikan adalah sebagai berikut: 74,51% dan 54,42% (TSS; 39,20% dan 49,12% (SS; 19,82% dan 14,2% (amoniak; dan 24,82% dan 22,47% (COD. Ternyata genteng plastik bergelombang mempunyai tingkat pengurangan (g/m3/hr dan tingkat pengurangan spesifik (mg/m2/hr terhadap kandungan amoniak lebih efektif bila dibandingkan dengan material lain seperti plastic rolls, scrub pads, pipa PVC dan lain sebagainya.   ABSTRACT Aquaculture has been developing rapidly during the last few decades; it is due to the increase of fish demand. Increasing aquaculture activities especially with semi-intensive and intensive system have significant effect on waste production, which has to be removed or to be reduced quickly because will effect on fish in rearing tank and environment when through away to environment such as river and sea. The objectives of this study were to know the capability of corrugated plastic to remove or to reduce wastes content produced by aquaculture activities, i.e, Total Suspended

  11. KORELASI ANTARA ISLAM DAN EKONOMI

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    Akmal Zainal Abidin

    2015-03-01

    Full Text Available CORELATION BETWEEN ISLAM AND ECONOMY. This paper aims to identifies about the correlation between economic and Islam. Islam is the perfect religion that governs all things in life, including the economy. This is evident with the concept of  well-being which is described in the al-Quran and Sunnah. Basically the goal of every human life is to prosper, although humans make sense of  well- being with a different perspective. Most understand economics assume that welfare is the welfare of  earthly material. But to make sense of well-being with the term al-Falah, is meaning holistic wellbeing and balance between material and spiritual dimensions. al-Quran and Sunnah have taught that the human being will be achieved if  living in balance between material and spiritual. This is because human life does not just stop in the life of this world, but there is still a second life that will be faced by humanity in the hereafter, and well-being will be achieved with the truth is that people can balance the needs of the world and the hereafter, and that is what is taught in Islamic economics. Keywords: Islam, Economy, Prosperity. Tulisan ini bertujuan untuk menjelasakn tentang korelasi antara ekonomi  dan  Islam.  Islam  adalah  agama  yang  sempurna  yang mengatur segala hal dalam kehidupan ini, termasuk juga ekonomi. Hal ini terbukti dengan konsep kesejahteraan yang dipaparkan dalam al-Quran dan Sunnah. Pada dasarnya tujuan hidup setiap manusia adalah untuk mencapai kesejahteraan, meskipun manusia memaknai kesejahteraan dengan perspektif  yang berbeda-beda. Sebagian besar paham ekonomi menganggap bahwa kesejahteraan adalah kesejahteraan material duniawi. Namun Islam memaknai kesejahteraan dengan istilah  Falah  yang  berarti  kesejahteraan  holistik  dan  seimbang antara dimensi material dan spiritual. Al-Quran dan Sunnah telah mengajarkan bahwa kesejahteraan akan tercapai jika manusia menjalani hidup secara seimbang antara material dan

  12. 荧光原位杂交技术在检测胎儿染色体亚显微结构异常中的应用%Application of the fluorescent in situ hybridization (FISH) on the prenatal diagnosis of the subtle chromosomal aberrations

    Institute of Scientific and Technical Information of China (English)

    尹爱华; 刘舒; 潘小英; 傅文婷; 王继成; 卢建; 杨洁霞

    2012-01-01

    Objective To study the diagnostic value of the fluorescent in situ hybridization on detection of subtle chromosomal aberrations in cultured amniotic fluid cells. Methods Amniocenteses were performed in a pregnant women of 18 gestational weeks, and metaphase FISH was followed because the results of amniotic fluid karyotype and ultrasound were disagree with each other. The metaphase chromosomes were hybridized in situ with the human centromere probes of chromosome 18,X,Y and site specific probe of chromosome 13,21. The treated slides were examined and taken photos under the carl zeiss fluoromicroscope. Result Subtle translocation of chromosome Y was detected by FISH. Conclusion Our result indicated that it is very helpful for the diagnosis of subtle chromosomal aberrations by combining FISH with traditional karyotype analysis.%目的 探讨荧光原位杂交(FISH)技术在诊断培养后的羊水细胞染色体亚显微结构异常中的应用价值.方法 对1例18孕周,经典细胞遗传学羊水染色体核型分析结果与B超检查结果有不符合的胎儿,应用FISH的18号、X、Y染色体着丝粒探针和13、21号染色体位点特异性探针,对培养后的羊水中期细胞标本进行检测.结果 共分析了22个独立细胞克隆的分裂象,发现胎儿染色体存在两种核型嵌合,结果记为:mos 45,X[20]/46,XY[2];FISH检测发现此胎儿核型存在Y染色体亚显微小片段易位.结论 FISH技术结合传统细胞遗传学核型分析,对于诊断染色体亚显微结构异常非常重要.

  13. KINERJA KEPALA SEKOLAH DALAM KEGIATAN BIMBINGAN DAN KONSELING

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    Abu Bakar M.Luddin

    2014-06-01

    Abstrak: Kinerja Kepala Sekolah dalam Kegiatan Bimbingan dan Konseling. Penelitian ini bertujuan mendeskripsikan kinerja kepala sekolah dalam pelaksanaan kegiatan bimbingan dan konseling. Penelitian dilaksanakan di SMU Negeri 2 Kota Binjai, dengan memergunakan rancangan deskriptif kualitatif. Subjek penelitian adalah kepala sekolah, koordinator bimbingan dan konseling, dan guru pembimbing. Data di­kumpulkan dengan observasi, wawancara mendalam, dan kajian dokumen, dan selanjutnya dianalisis sete­lah melalui proses triangulasi antarsubjek dan informasi. Temuan penelitian menunjukkan bahwa kinerja kepala sekolah terkait dengan pelaksanaan kegiatan bimbingan dan konseling masih belum sepenuhnya sebagaimana yang diharapkan. Kepala sekolah perlu meningkatkan kinerjanya dalam menjalankan fungsi koordinasi dan kepengawasan untuk mencapai kegiatan bimbingan dan konseling yang efektif.

  14. New developments in comet-FISH.

    Science.gov (United States)

    Spivak, Graciela

    2015-01-01

    The comet assay combined with fluorescence in-situ hybridisation (FISH) is a powerful technique for comparative analyses of damage induction and repair in genomes and in specific DNA sequences within single cells. Recent advances in the methodology of comet-FISH will be considered here, with particular attention to the design and generation of fluorescent probes. In general, all the approaches must fulfil a few basic requirements: the probes should be no longer than ~300 nucleotides in length (single or double stranded) to be able to penetrate the gel in which the target genomic DNA is embedded, they should be sequence-specific, and their signal should be detectable and distinct from the background fluorescence and the dye used to stain the DNA.

  15. STRATEGI PEGELOLAAN SUAKA PERIKANAN RAWA BANJIRAN DI SUMATERA DAN KALIMANTAN

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    Agus Djoko Utomo

    2016-10-01

    “Danau lindung Empangau “ (124 ha di Kapuas Hulu Kalimantan Barat terdapat stok ikan 5.700 ekor/ha didominansi oleh ikan Haruan (Channa striata, Entukan (Thynnichthys thynnoides, Biawan (Helostoma temmenckii, Tengadak (Barbonymus schwanenfeldii. Suaka perikanan tipe sungai “Kapak Hulu” (segmen sungai panjang 1 km, lebar 30 m di Lubuk Lamam Sumatera selatan terdapatstok ikan 4 ton didominansi oleh ikan Lais (Kryptopterus kryptopterus, Baung (Hemibagrus nemurus, Palau (Osteochilus hasselti, Lampam (Barbonymus schwanenfeldii. Suaka perikanan “Lubuk Gunung Isam” di Lubuk Lamam Sumatera selatan terdapat stok ikan 2 ton didominansi oleh ikan Tapa (Wallago leeri, Bulu Tulang (Kryptopterus apagon, Baung (Hemibagrus nemurus, Lais (Kryptopterus kryptopterus. Agar supaya suaka perikanan dapat berfungsi dengan baik sehingga mempunyai dampak terhadap masyarakat sekitarnya maka suaka tersebut harus dikelola dengan tepat mulai dari penentuan lokasi yang tepat, sarana prasarana yang diperlukan, serta kelembagaan dan pengawasan. Floodplain waters are strongly influenced by season, drought during dry season and floods in rainy season. There are two groups of fish in the swamp namely the swamp fishgroup (black fish and river fish group (white fish. Based on the type of habitat in the swamp flood, there are several types of fish reserves namely floodplain pool reserve, swamp lake reserve, river segment reserve, and deep pool of the riverreserve. Floodplain pool reserve type:” Suak Buaya Reserve”(0.5 ha in southern Sumatra contained 2 tons of fish stocks dominated by snakehead, kissing gourami, Snakeskin gourami, climbing perches. Swamp lake reserve type: Empangau Lake Reserve (124 ha in West Kalimantan with5.700 fish/ha dominated by Snakehead fish, minnows or carp,kissing gourami, Tinfoil barb. River segment reserve type: “Kapak Hulu Reserve “ (river segment, length =1 km and width =30 m in southern Sumatra contained 4 tons of fish dominated by Sheatfishes, Catfish

  16. IKLIM SEKOLAH DAN EFIKASI KENDIRI DALAM KALANGAN GURU BIMBINGAN DAN KAUNSELING SEKOLAH MENENGAH DI KELANTAN

    OpenAIRE

    Ibrahim, Hasliza; Ismail, Siti Noor; Kassim, Mohamad Adnan Mohamad; Mohamad, Suhaila

    2017-01-01

    Artikel ini bertujuan untuk mengenal pasti hubungan antara iklim sekolah dan efikasi kendiri guru bimbingan dan kaunseling di negeri Kelantan.  Responden kajian adalah terdiri daripada 181 orang guru bimbingan dan kaunseling Sekolah Menengah di negeri Kelantan.  Kajian rintis dijalankan ke atas 30 orang guru bimbingan dan kaunseling di daerah Tanah Merah.  Secara spesifiknya, kaedah kuantitatif digunakan untuk tujuan pengumpulan data.  Maklum balas melalui soal selidik telah diperolehi dan se...

  17. Kandidiasis di Mulut akibat Khemoterapi dan Penatalaksanaannya

    OpenAIRE

    2016-01-01

    Latar belakang: kandidiasis (kandidosis) adalah infeksi jamur yang disebabkan oleh spesies Candida biasanya Candida albicans. Faktor predisposisi yang memicu kandidiasis adalah terganggunya ekologi mulut karena antara lain pemakaian antibiotika, kortikosteroid, penyakit kronis dan keganasan, beberapa gangguan darah; terapi radiasi di leher dan kepala; khemoterapi; leukimia, obat sitotoksik, juga kebersihan mulut yang buruk. Tujuan: melaporkan kasus kandidiasis di mulut karena khemoterapi dan ...

  18. PEMBELAJARAN SENI TARI: AKTIF, INOVATIF DAN KREATIF

    OpenAIRE

    Gandes Nurseto

    2015-01-01

    Pendidikan seni tari perlu diberikan pada Sekolah Dasar karena keunikan, kebermaknaan terletak pada pemberian pengalaman estetik dalam bentuk berapresiasi dan menggunakan model pembelajaran yang PAIKEM Pembelajaran, Aktif, Inovatif, Kreatif, Efektif dan Menyenagkan dalam proses pembelajaran didalam kelas dari Standar Kompetensi dan Komptensi Dasar mengapresiasi karya seni tari tunggal nusantara yang bertujuan untuk mengenalkan karya seni tari daerah lain. Masalah dalam penelitian ini bagaiman...

  19. Fish Tales

    Energy Technology Data Exchange (ETDEWEB)

    McLerran, L.

    2010-07-06

    This talk is about fishing and the friendships that have resulted in its pursuit. It is also about theoretical physics, and the relationship of imagination and fantasy to the establishment of ideas about nature. Fishermen, like theoretical physicists, are well known for their inventive imaginations. Perhaps neither are as clever as sailors, who conceived of the mermaid. If one doubts the power of this fantasy, one should remember the ghosts of the many sailors who drowned pursuing these young nymphs. An extraordinary painting by J. Waterhouse is shown as Fig. 1. The enchantment of a mermaid must reflect an extraordinary excess of imagination on the part of the sailor, perhaps together with an impractical turn of mind. A consummated relationship with a mermaid is after all, by its very nature a fantasy incapable of realization. To a theoretical physicist, she is symbolic of many ideas we develop. There are many truths known to fisherman in which one might also find parallels to the goals of scientists: (1) A fish is the only animal that keeps growing after its death; (2) Nothing makes a fish bigger than almost being caught; (3) ''...of all the liars among mankind, the fisherman is the most trustworthy.'' (William Sherwood Fox, in Silken Lines and Silver Hooks); and (4) Men and fish are alike. They both get into trouble when they open their mouths. These quotes may be interpreted as reflecting skepticism regarding the honesty of fisherman, and probably do not reflect adequate admiration for a creative imagination. Is it fair to criticize a person for believing a falsehood that he or she sincerely believes to be true? The fisherman simultaneously invents the lie, and believes in it himself. The parallel with theoretical physics is perhaps only approximate, although we physicists may invent stories that we come to believe, on some rare occasions our ideas actually correspond to a more or less true descriptions of nature. These minor philosophical

  20. Fish Immunoglobulins

    Science.gov (United States)

    Mashoof, Sara; Criscitiello, Michael F.

    2016-01-01

    The B cell receptor and secreted antibody are at the nexus of humoral adaptive immunity. In this review, we summarize what is known of the immunoglobulin genes of jawed cartilaginous and bony fishes. We focus on what has been learned from genomic or cDNA sequence data, but where appropriate draw upon protein, immunization, affinity and structural studies. Work from major aquatic model organisms and less studied comparative species are both included to define what is the rule for an immunoglobulin isotype or taxonomic group and what exemplifies an exception. PMID:27879632

  1. KOMPOSISI UKURAN, NISBAH KELAMIN DAN DAERAH PENYEBARAN HIU BUAYA (Pseudocarcharias kamoharai YANG TERTANGKAP DI SAMUDERA HINDIA

    Directory of Open Access Journals (Sweden)

    Dian Novianto

    2016-05-01

    Full Text Available Ikan hiu buaya (Pseudocarcharias kamoharai merupakan salah satu jenis hiu yang tertangkap secara tidak sengaja pada perikanan rawai tuna (tuna longline. Ikan hiu buaya dalam penanganannya selalu dibuang di laut karena ukuran tubuhnya yang kecil dan tidak memiliki pasar diantara ikan non target (by-catch lainnya. Penelitian tentang komposisi ukuran, nisbah kelamin dan daerah penyebaran hiu buaya dilakukan pada tahun 2010 – 2011 di perairan WPP 573 – 572 Samudera Hindia dengan mengikuti kapal longline yang berbasis di Benoa dan Bungus. Tujuan penelitian adalah untuk mendapatkan data dan informasi dalam rangka pengelolaan dan konservasi hiu buaya. Hiu buaya memiliki kisaran panjang tubuh 37 – 96 cm FL dengan panjang maksimal 96 cm FL untuk jenis betina dan 94 cm FL untuk jenis jantan dan modus pada kisaran 85 – 89 cm FL untuk jenis betina dan 75 – 79 cm FL untuk jenis jantan. Perbandingan jenis kelamin ikan jantan dan betina adalah 1 : 1,33, menunjukkan bahwa rasio ikan jantan dan betina tidak seimbang. Ikan hiu buaya terdistribusi tidak merata, hanya terdapat di 77 posisi setting pada 1120 – 1220 Bujur Timur dan 090 – 150 Lintang Selatan dari 256 jumlah setting selama periode penelitian ini.   Crocodile shark (Pseudocarcharias kamoharai is one of sharks species caught accidentally in tuna longline fisheries. Crocodile shark handling is always discarded at sea because of it is small size and is not economics value among others non-target fish (by-catch. Research on the biology of crocodile shark was conducted in 2010 – 2011 at Fisheries management Areas (FMA 572 (Indian Ocean – west Sumatera and 573 (South of Java – East Nusa Tenggara following longline vessels based in Benoa and Bungus. The purpose of research was to investigate data and information on the crocodile shark for management and conservation purposes. Crocodile shark has a range of body length of 37-96 cm FL with a maximum length of 96 cm FL for females and 94 cm

  2. APPLICATION OF RE-ESTABLISHING DOSE-RESPONSE CURVES BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH)%荧光原位杂交技术在剂量重建中的应用

    Institute of Scientific and Technical Information of China (English)

    段志凯; 张淑贤; 郭万龙; 吴启庆; 郭鲜花; 时爱丽; 王斌生

    2008-01-01

    采用不同剂量60Co γ射线照射两名正常人外周血淋巴细胞后,应用荧光原位杂交(FISH)技术检测染色体易位率,并拟合出剂量效应关系曲线为:Y=0.01316+0.07569D+0.01583D2.通过盲法对实验结果验证,验证结果表明拟合的曲线可用于剂量重建.FISH技术用来检测染色体稳定性畸变,既快速又准确,适合于染色体易位的检测,有望成为剂量重建的理想技术方法.

  3. 荧光原位杂交(FISH)技术在转基因小鼠检测中的应用%Application of the technique of fluorescence in situ hybridization in detecting transgenic mice

    Institute of Scientific and Technical Information of China (English)

    谢建云; 邵伟娟; 潘漪清; 高诚

    2002-01-01

    制备转基因小鼠特有的DIG标记探针,应用染色体荧光原位杂交(FISH)技术,对转基因小鼠外周血细胞的遗传性状进行分析,检测其合子类型,以挑选纯合子小鼠用于保种.结果杂合型小鼠61%的细胞有一个荧光信号,而纯合型小鼠约24%的细胞有两个荧光信号,48%的细胞有一个荧光信号,野生型小鼠无荧光信号.因此染色体荧光原位杂交技术可作为检测转基因小鼠遗传特性的一种方法.

  4. CLINICAL STUDY OF CHROMOSOMAL ABNORMALITY IN MISSED ABORTION TISSUE DETECTED BY FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE%FISH 技术检测稽留流产组织中染色体异常的临床研究

    Institute of Scientific and Technical Information of China (English)

    王春珺; 于晶峰

    2014-01-01

    目的:探讨稽留流产胎儿组织细胞中染色体异常总发生率及异常种类和各种异常的发生率。方法:采用18号、X 和 Y 染色体着丝粒探针及13、16、21、22号染色体单一序列探针,对105例稽留流产胎儿组织进行FISH 检测。结果:49.5%的稽留流产是由胎儿染色体异常引起的;染色体异常的前3位为三倍体,16号染色体三体,X 单体,分别占染色体异常总发生率的32.7%、15.4%和15.4%;高龄组(≥35岁)和非高龄组(0.05);妊娠≤12wk和妊娠>12wk者染色体异常率分别为60.9%和31.7%,差异有统计学意义(P0. 05). The detection rate of chromosomal abnormality in the cases of ≤12 gestational weeks was 60. 9%,while the detection rate of chromosomal abnormality in the cases of >12 gestational weeks was 31. 7%,there was significant difference(P<0. 05). Conclusion:Most missed abortion are caused by genetic gene defect, FISH technique can detect aborted fetuses, find chromosomal abnormality rapidly and accurately,provide data for genetic counseling of subsequent pregnancy.

  5. STATUS TROFIK DAN ESTIMASI POTENSI PRODUKSI IKAN DI PERAIRAN DANAU TEMPE, SULAWESI SELATAN

    Directory of Open Access Journals (Sweden)

    Samuel Samuel

    2016-03-01

    Full Text Available Danau Tempe merupakan tipe danau rawa banjiran yang dikenal sebagai danau yang banyak menghasilkan ikan air tawar di Propinsi Sulawesi Selatan. Penelitian status trofik dan estimasi potensi produksi ikan di Danau Tempe dilakukan pada bulan Pebruari-Nopember 2010, bertujuan untuk mengetahui kondisi terkini tentang status trofik dan potensi produksi ikan pada perairan danau. Penelitian bersifat survei lapangan dan analisis di laboratorium. Survei dilakukan sebanyak 4 kali mewakili musim kemarau dan musim penghujan. Pengukuran parameter kualitas air dilaksanakan di sepuluh stasion yang dipilih secara purposif. Hasil penelitian menunjukkan bahwa kualitas air Danau tempe masih ideal mendukung kehidupan dan perkembanganbiakan ikan serta organisme air lainnya sebagai pakan ikan.  Status trofik perairan Danau Tempe sesuai kriteria Trophic Status Index, mempunyai indeks rata-rata 56,6 - 59,8 dengan status “eutrofik ringan”, ditandai melimpahnya tumbuhan air di perairan danau.  Angka potensi produksi ikan berkisar antara 69-148 kg/ha/tahun dengan nilai rata-rata 95 kg/ha/tahun. Dengan luasan Danau Tempe antara 15.000-20.000 hektar menghasilkan produksi ikan antara 1428 -1904 ton/tahun.   Lake Tempe is a floodplain lake that produces a lot of freshwater fish in South Sulawesi Province. Research on trophic status and estimation of potential fish production in Lake Tempe was conducted from February-November, 2010. Aim of this study was to obtain information on the current condition of the trophic status and potential fish production in the lake waters. This study is based on the field survey and analysis in the laboratory. Surveys were conducted  4 times to represent the dry and rainy seasons, and water quality parameter measurements were carried out at ten stations selected purposively. The results shows that water quality parameters of Lake Tempe was still quite ideal to support aquatic life and the development of fish and other aquatic organisms

  6. IMPLEMENTASI TQM BERORIENTASI HARD SKILL DAN SOFT SKILL DALAM PEMBELAJARAN SEJARAH SMA DI KOTA SEMARANG

    Directory of Open Access Journals (Sweden)

    Cahyo Budi Utomo

    2011-10-01

    Full Text Available This study aims to introduce the implementation of hardskill and softskill oriented TQM in teacher’s learning of history in Senior High School in Semarang. The research model are structural fit model and significance of relationship between TQM implementation model, PDSA cycle, the model analysis of fish bone, continuous improvement , hard skills and soft skills and qualified history learning is the main target of research. Data was obtained from field studies using Likert scale questionnaire developed from the constructs and theoretical construct indicator. Analysis performed using software models SmartPLS. Implementation of PDSA cycle correlated 0879 to the implementation of fish bone analysis, correlates 0830 PDSA cycle to continuously improvement , while continuously improvement  correlated 0441 and 0749 against the hard skills to soft skills. Soft skills to hard skills correlate of 0329, while the correlation of hard skills to qualified history learning is at 0673. The developed qualityfied history learning need support from  the realization of the continuous (continuously improvement and optimal orientation of hard skills. Keywords: model, TQM, PDSA, the analysis of fish bone, hard skills, soft skills, learning the history of quality  Kajian ini bertujuan mengenalkan model implementasi TQM yang berorientasi pada hardskill dan softskill dalam pembelajaran sejarah di sekolah atas Semarang pada para guru. Sebagai model penelitian, model struktural fit dan signifikasi  dari korelasi antara model implementasi TQM, siklus PDSA, model analisis tulang ikan, perbaikan terus-menerus, hard skill dan soft skill, dan pembelajaran sejarah bermutu (PSB menjadi target utama penelitian. Data didapatkan dari studi lapangan menggunakan angket skala Likert yang dikembangkan dari konstruk dan indicator konstruk teoretis. Analisis menggunakan program SmartPLS. Implementasi siklus PDSA berkorelasi 0879 terhadap implementasi model analisis

  7. POLA USAHA DAN PENDAPATAN RUMAH TANGGA PETANI PADA BERBAGAI TIPOLOGI LAHAN RAWA LEBAK

    Directory of Open Access Journals (Sweden)

    Nasir Nasir

    2015-11-01

    Full Text Available The study aim to 1 Describing of business pattern developed farmer households in various typologies of lowland swamp land, and 2 Analyzing household income of farmers in various businesses developed in lowland swamp land. The sampling method used is a simple random sample with a total of  responden  as many as 222 farmers. The study used a survey method. The results showed that the business pattern of households at shallow swampy, ie: Horticulture: annual fruit, Livestock: chickens and goats, trade, household industry, transport services, Rice, fishing, horticulture: vegetables and fruits a season and crops: cassava and peanuts, Labour, fish farming. The business pattern of households at middle swamp, ie: horticulture: annual fruit, Livestock: ducks, trade, household industry, transport services, rice, fishing, horticulture: vegetables and fruits a season, Labor, fish farming. The business pattern of households at deep swampy, ie: Horticulture: annual fruit, Livestock: duck and buffalo, trade, household industry, transport services, rice, fishing, horticulture: vegetables and fruits a season, Labor, fishing, fish farming. Household income in each of the  swampy land, ie: household income  the shallow swampy amounted Rp19.525.400/year.  The household income  the middle swampy amounted Rp20.212.000/year.  The household income the deep swampy amounted Rp18.248.000/yearKeywords: pattern of business, income, household, lebak, surveyABSTRAKTujuan penelitian ini 1 mendeskripsikan pola usaha yang dikembangkan rumah tangga petani pada berbagai tipologi lahan rawa lebak, dan 2 menghitung pendapatan rumah tangga petani pada berbagai pola usaha yang dikembangkan pada lahan rawa lebak. Metode penarikan contoh yang digunakan adalah sampel acak sederhana (random sampling dengan jumlah total sebanyak 222 orang petani. Metode penelitian yang digunakan adalah metode survei. Hasil penelitian menunjukan bahwa pola usaha yang dikembangkan petani lahan rawa

  8. Fishing activities

    Science.gov (United States)

    Oberle, Ferdinand; Puig, Pere; Martin, Jacobo; Micallef, Aaron; Krastel, Sebastian; Savini, Alessandra

    2018-01-01

    Unlike the major anthropogenic changes that terrestrial and coastal habitats underwent during the last centuries such as deforestation, river engineering, agricultural practices or urbanism, those occurring underwater are veiled from our eyes and have continued nearly unnoticed. Only recent advances in remote sensing and deep marine sampling technologies have revealed the extent and magnitude of the anthropogenic impacts to the seafloor. In particular, bottom trawling, a fishing technique consisting of dragging a net and fishing gear over the seafloor to capture bottom-dwelling living resources has gained attention among the scientific community, policy makers and the general public due to its destructive effects on the seabed. Trawling gear produces acute impacts on biota and the physical substratum of the seafloor by disrupting the sediment column structure, overturning boulders, resuspending sediments and imprinting deep scars on muddy bottoms. Also, the repetitive passage of trawling gear over the same areas creates long-lasting, cumulative impacts that modify the cohesiveness and texture of sediments. It can be asserted nowadays that due to its recurrence, mobility and wide geographical extent, industrial trawling has become a major force driving seafloor change and affecting not only its physical integrity on short spatial scales but also imprinting measurable modifications to the geomorphology of entire continental margins.

  9. Deep Fish.

    Science.gov (United States)

    Ishaq, Omer; Sadanandan, Sajith Kecheril; Wählby, Carolina

    2017-01-01

    Zebrafish ( Danio rerio) is an important vertebrate model organism in biomedical research, especially suitable for morphological screening due to its transparent body during early development. Deep learning has emerged as a dominant paradigm for data analysis and found a number of applications in computer vision and image analysis. Here we demonstrate the potential of a deep learning approach for accurate high-throughput classification of whole-body zebrafish deformations in multifish microwell plates. Deep learning uses the raw image data as an input, without the need of expert knowledge for feature design or optimization of the segmentation parameters. We trained the deep learning classifier on as few as 84 images (before data augmentation) and achieved a classification accuracy of 92.8% on an unseen test data set that is comparable to the previous state of the art (95%) based on user-specified segmentation and deformation metrics. Ablation studies by digitally removing whole fish or parts of the fish from the images revealed that the classifier learned discriminative features from the image foreground, and we observed that the deformations of the head region, rather than the visually apparent bent tail, were more important for good classification performance.

  10. STATUS STOK, EKSPLOITASI DAN OPSI PENGELOLAAN SUMBERDAYA IKAN TUNA DI LAUT BANDA

    Directory of Open Access Journals (Sweden)

    Agustinus Anung Widodo

    2015-05-01

    Full Text Available Laut Banda merupakan salah satu daerah penangkapan tuna yang potensial di Indonesia, Jenis alat tangkap yang digunakan terdiri dari pukat cincin, huhate, rawai tuna, pancing ulur dan pancing tonda. Hasil tangkapan tuna di Laut Banda meliputi cakalang, madidihang dan tuna mata besar. Sumberdaya tuna di Laut Banda diduga masih merupakan sub stok sumberdaya tuna di perairan Pasifik Tengah dan Barat. Hasil kajian stok tuna oleh Western and Central Pacific Fisheries Commission (WCPFC pada 2012 melaporkan bahwa stok cakalang dan madidihang tidak mengalami overfishing dan overfished, sedangkan tuna mata besar telah mengalami overfishing dan overfished. Hasil penelitian Pusat Penelitian Pengelolaan Perikanan dan Konservasi Sumber Daya Ikan (P4KSI pada 2012 menunjukkan madidihang dan tuna mata besar tertangkap pukat cincin, huhate serta kombinasi pancing ulur permukaan dan pancing tonda pada stadium yuwana, dengan indikasi nilai LcFmsy while YFT and SKJ are not in overfishing or overfished state. It was also reported by RCFMC that the size of catch of those species by various fishing gear indicating that value of Lcfishing practice for resource sustainability it is suggestted for tuna management in Banda sea should be not to develop and increase the effort for purse seine and may shift to pole line, hand line and/or troll line are still openated.

  11. INTERAKSI VARIABILITAS IKLIM DENGAN EKOSISTEM TERUMBU KARANG DAN SUMBER DAYA IKAN KARANG

    Directory of Open Access Journals (Sweden)

    Reny Puspasari

    2015-12-01

    Full Text Available Keberadaan ikan karang di perairan dapat dipengaruhi oleh beberapa faktor, antara lain: kondisi ekosistem terumbu karang, lingkungan perairan di sekitarnya dan perubahan iklim. Variabilitas iklim ditandai oleh besar kecilnya nilai dari Southern Oscillation Index (SOI, Dipole Mode Index (DMI dan curah hujan. Hubungan antara variabilitas iklim dengan ekosistem terumbu karang dan sumber daya ikan karang di wilayah perairan Sumatera Barat dianalisis dengan menggunakan analisis deskriptif terhadap data temporal, analisis komponen utama dan uji-t pada nilai CPUE (catch per unit effort  ikan karang dari tahun-tahun terjadinya La Nina dan El Nino. Hasil penelitian menunjukkan bahwa SOI mempengaruhi kondisi ekosistem terumbu karang dan nilai CPUE ikan karang. Pemutihan karang di perairan Sumatera Barat pada 1997 terjadi ketika peristiwa El Nino Ekstrim negatif yang berasosiasi dengan IODM (Indian Ocean Dipole Mode positif kuat. Nilai CPUE ikan karang lebih tinggi pada saat terjadi La Nina bila dibandingkan dengan nilai saat peristiwa El Nino. Rata-rata CPUE ikan karang pada saat terjadi peristiwa La Nina adalah 67 kg/trip, sementara pada saat terjadi peristiwa El Nino rata-rata nilai CPUE ikan karang adalah 32 kg/trip. Faktor DMI mempengaruhi peningkatan konsentrasi klorofil-a perairan sebagai tanda terjadi peningkatan kesuburan perairan. Status of reef fishes are highly influenced by several factors, such as: coral reef condition, adjacent waters condition and climate change. Climate variability is indicated by the values of Southern Oscillation Index (SOI, Dipole Mode Index (DMI and rain fall. Interaction between climate variability and coral reef ecosystem and reef fish condition were analyzed by using a descriptive analysis to the temporal data, principal component analysis and t-test for CPUE of reef fishes in a certain years when La Nina and El Nino occurred. The results show that SOI influenced the coral reef ecosystem and reef fishes CPUE. Coral

  12. Pengaturan dan Pengawasan pada Bank Syariah

    Directory of Open Access Journals (Sweden)

    Ali Syukron

    2012-06-01

    Full Text Available Peran bank dalam melaksanakan tugas dan fungsinya perlu diatur secara baik dan benar. Hal ini bertujuan untuk menjaga kepercayaan nasabah terhadap aktivitas perbankan. Salah satu peraturan yang perlu dibuat untuk mengatur perbankan adalah peraturan mengenai permodalan bank yang berfungsi sebagai penyangga terhadap kemungkinan terjadinya kerugian. Tulisan ini merupakan studi pustaka dimana penulis mengelaborasi bagaimana pengaturan dan pengawasan pada bank syariah yang ada di berbagai negera. Dalam tulisan ini, penulis mengambil sampel negara Malaysia,  Pakistan, Kuwait, dan Inggris. Alasan memilih keempat negara tersebut karena Malaysia dan Kuwait memiliki kemiripan dengan Indonesia yang mengadopsi dual banking system.

  13. ANALISIS PEMBELAJARAN HUMANIS DAN KONSTRUKTIVISKARAKTER, DAN KEMAMPUAN LITERASI MATEMATIKA

    Directory of Open Access Journals (Sweden)

    Muhammad Jazimul Ni’am

    2014-02-01

    Full Text Available AbstrakTujuan penelitian ini adalah untuk mengetahui pencapaian indikator teori humanismedankonstruktivismedalamperencanaan, bahanajar,danprosespembelajaranmateritrigonometri. Selainitu, ingindiketahui kemampuanliterasi matematikadankarakteryangmunculsebagaihasilbelajar.Penelitianinimerupakanpenelitiankualitatif.Instrumen yang disusun dan dikembangkan terdiri dari instrumen penila-ian perangkat pembelajaran,instrumen penilaian proses pembelajaran, tes kemampuanliterasimatematika,daninstrumenpenilaiankarakter.Validitasinstrumenpenelitianditentukanberdasarkan validasitimahli dan hasil tes uji cobadi lapangan.Teknikpengumpulandata yaitudenganmetode tes,observasi,wawancara,dan angket.Analisisdatadilakukan sebelum,selama, dan setelah penelitianberupaanalisisdiskriptif kualitatif.Keabsahan data hasil penelitian diuji menggunakan metode trianggulasidata. PenilaianterhadapRPP dan proses pembelajaranberada pada levelyangrendah, penilaian bahan ajar tergolong sedang jika dilihat daritiga aspek yaitupembelajaranhumanis,konstruktivis,dankemampuanliterasimatematika.Pencapaiankemampuan literasi matematikamasihdalam levelmenerjemahkanmasalahkontekstualkemodelmatematika dan belummampumencapai tahap menerapkandanmenafsirkan.Ketujuhkarakteryangdiamati belumsepenuhnyaterlihat. Karakteryangterlihat selamapembelajaran adalah jujur,toleransi,tanggungjawab,rasaingintahu, dan percayadiri.AbstractThe purpose of  this study was to determine the achievement indicators humanism andconstructivism theory in planning, teaching materials, and learning materials in matter trigonometry.In addition, it will be analyzedthe mathematical literacyskills andcharacterthatappearsas a resultof learning. This research is qualitative. The instrument was conceived anddeveloped is instrument to assess of  learning tools such as lesson plans and teaching materials, learning assessment, tests of  mathematical literacy skills, and instrument of  character. Validi-ty instruments of

  14. STRUKTUR DAN PRODUKSI LEBAH Trigona spp. PADA SARANG BERBENTUK TABUNG DAN BOLA

    Directory of Open Access Journals (Sweden)

    Putu Ade Hinduari Putra

    2015-12-01

    Full Text Available Lebah tanpa sengat (Trigona spp. dapat menghasilkan madu yang bermanfaat bagi kesehatan. Penelitian bertujuan untuk mengetahui : (1 morfologi Trigona spp., (2 struktur internal sarang Trigona spp. pada sarang berbentuk tabung dan bola, (3 volume sarang serta perkiraan produksi madu, beebread dan selanakan Trigona spp. pada sarang berbentuk tabung dan bola. Penelitian dilaksanakan pada bulan November 2014. Sampel yang digunakan adalah koloni dan sarang Trigona spp. berbentuk tabung dan bola yang diambil di Desa Padang Tunggal,Kecamatan Selat, Kabupaten Karangasem, Bali. Hasil penelitian menunjukan bahwa nama spesies dari sampel koloni Trigona spp. pada sarang berbentuk tabung dan bola adalah Trigona laeviceps. Struktur internal sarang Trigona spp. pada sarang berbentuk tabung dan bola mempunyai pola susunan yaitu pot madu, pot beebread dan pot sel anakan. Volume sarang besar memberikan perkiraan total jumlah produksi madu, beebread dan selanakan lebih banyak dibandingkan volume sarang kecil.

  15. PERAN KEPALA SEKOLAH DAN PUSTAKAWAN DALAM PEMBERDAYAAN PERPUSTAKAAN SD MUHAMMADIYAH SAPEN DAN SD NEGERI GIWANGAN

    Directory of Open Access Journals (Sweden)

    Widya Setyaningrum Bagyoastuti

    2016-04-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan: (1 peran kepala sekolah dalam memberdayakan perpustakaan sekolah dasar, dan (2 peran pustakawan dalam memberdayakan perpustakaan sekolah dasar. Pendekatan kualitatif digunakan dalam penelitian ini dengan menjadikan perpustakaan SD Muhammadiyah Sapen dan SD Negeri Giwangan sebagai subjek penelitian. Data penelitian dikumpulkan melalui teknik observasi, wawancara, dan dokumentasi kemudian dianalisis dengan mengacu pada model interaktif menurut Miles dan Huberman. Hasil penelitian menunjukkan bahwa pemberdayaan perpustakaan sekolah dasar dimulai manakala terdapat dukungan kepala sekolah dan hadirnya pustakawan penuh waktu yang profesional. Kepala sekolah berperan sebagai (1 manajer sekolah, (2 pemimpin instruksional, dan (3 agen perubahan. Pustakawan berperan sebagai (1 manajer perpustakaan, (2 ahli informasi dan penggiat literasi informasi, (3 pelaku instruksional, dan (4 kolaborator. Pemberdayaan perpustakaan dapat dilakukan dengan (1 meningkatkan partisipasi warga sekolah dalam pengembangan koleksi pustaka, (2 meningkatkan kolaborasi antara pustakawan dengan guru dalam pembelajaran, dan (3 pengembangan literasi informasi yang terintegrasi ke dalam pembelajaran.

  16. ETIKA BISNIS AL-GHAZALI DAN ADAM SMITH DALAM PERSPEKTIF ILMU BISNIS DAN EKONOMI

    Directory of Open Access Journals (Sweden)

    AM. M. Hafidz MS.

    2013-05-01

    Full Text Available Penelitian ini adalah untuk mengeksplorasi: (1 pembangunan etika bisnis yang telah dirumuskan oleh al-Ghazali dan Adam Smith, (2 persamaan dan perbedaan antara dua etika bisnis pria, dan (3 relevansi bisnis mereka etika bisnis modern dunia dan ekonomi. Bisnis etika dibangun oleh al-Ghazali dan Smith di dataran praksis tidak jauh berbeda. Etika bisnis konstruksi dibangun oleh al-Ghazali didasarkan pada prinsip-prinsip seperti orientasi itikad baik tentang dunia dan akhirat, kejujuran, kepentingan pribadi dan social keseimbangan, dan perilaku / perbuatan yang tepat. Di sisi lain, etika bisnis konstruksi dibangun oleh Smith, berdasarkan keadilan, altruisme, keadilan dan liberal (kebebasan ekonomi. Baik etika bisnis yang diperkenalkan oleh kedua sangat relevan untuk menjadi digunakan sebagai bahan pokok acuan dalam etika bisnis modern.

  17. PENGARUH PEMBERIAN HIJAUAN DAN KONSENTRAT MENGANDUNG UREA-KAPUR DAN UBI KAYU TERHADAP PENAMPILAN KAMBING PE

    Directory of Open Access Journals (Sweden)

    I G. Mahardika

    2015-06-01

    Full Text Available Penelitian telah dilakukan untuk mengetahui pengaruh pemberian hijauan dan konsentrat yang mengandung urea-kapur dan ubi kayu terhadap produktivitas kambing. Penelitian menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 4 ulangan. Ke empat perlakuan yang dicobakan adalah Perlakuan A: ransum dengan 75% konsentrat (mengandung 4% urea, 2% kapur dan 50% ubikayu dan 25% hijauan (40% gamal dan 60% rumput raja, perlakuan B: rasnsum yang terdiri 60% konsentrat 40% hijauan, perlakuan C: ransum dengan 45% konsentrat dan 55% hijauan dan perlakuan D: ransum dengan 30% konsentrat dan 70% hijauan. Hasil penelitian mendapatkan bahwa produktivitas kambing yang mendapat ransum dengan level konsentrat 45% sampai 75% tidak berbeda sedangkan yang mendapat ransum dengan level konsentrat 30% lebih rendah. Ransum yang memebrikan nilai ekonomi tertinggi adalah ransum yang mengandung konsentrat antara 45% sampai 60%.

  18. IDENTIFIKASI DAN TEKNIK PENGENDALIAN HAMA DAN PENYAKIT BENIH PULAI (Alstonia scholaris

    Directory of Open Access Journals (Sweden)

    Evayusvita Rustam

    2016-09-01

    Full Text Available Tujuan dari penelitian ini adalah mengidentifikasi dan mengendalikan hama dan penyakit benih pulai yang terbawa dari lapangan. Identifikasi cendawan dilakukan dengan menginkubasi benih selama 7 hari, sedangkan untuk pengendalian terhadap hama dan penyakit benih diberi insektisida nabati dan kimia. Dari hasil identifikasi jenis cendawan pada benih pulai yaitu Aspergillus sp, Curvularia sp., Fusarium sp., Penicillium sp. dan Rhizopus sp. Persentase infeksi cendawan tertinggi pada benih pulai asal Nagrak (Aspergillus sp. sebesar 92%, Curvularia sp. 29% dan Fusarium sp. 21%, sedangkan persentase infeksi cendawan terendah pada benih pulai asal Jambi (Aspergillus sp. 2% dan Fusarium sp. 1%. Perlakuan terbaik yang dapat mengendalikan hama pada benih pulai adalah perlakuan yang diberi ekstrak daun suren dalam wadah plastik tertutup dan disimpan di ruang suhu kamar 270C selama 2 bulan. Pengendalian penyakit, terbaik pada benih adalah memberi bubuk kunyit ke dalam wadah plastik tertutup dan disimpan di lemari es 160C. Perlakuan tersebut menghasilkan daya kecambah masing-masing 70%.

  19. Fishing amplifies forage fish population collapses.

    Science.gov (United States)

    Essington, Timothy E; Moriarty, Pamela E; Froehlich, Halley E; Hodgson, Emma E; Koehn, Laura E; Oken, Kiva L; Siple, Margaret C; Stawitz, Christine C

    2015-05-26

    Forage fish support the largest fisheries in the world but also play key roles in marine food webs by transferring energy from plankton to upper trophic-level predators, such as large fish, seabirds, and marine mammals. Fishing can, thereby, have far reaching consequences on marine food webs unless safeguards are in place to avoid depleting forage fish to dangerously low levels, where dependent predators are most vulnerable. However, disentangling the contributions of fishing vs. natural processes on population dynamics has been difficult because of the sensitivity of these stocks to environmental conditions. Here, we overcome this difficulty by collating population time series for forage fish populations that account for nearly two-thirds of global catch of forage fish to identify the fingerprint of fisheries on their population dynamics. Forage fish population collapses shared a set of common and unique characteristics: high fishing pressure for several years before collapse, a sharp drop in natural population productivity, and a lagged response to reduce fishing pressure. Lagged response to natural productivity declines can sharply amplify the magnitude of naturally occurring population fluctuations. Finally, we show that the magnitude and frequency of collapses are greater than expected from natural productivity characteristics and therefore, likely attributed to fishing. The durations of collapses, however, were not different from those expected based on natural productivity shifts. A risk-based management scheme that reduces fishing when populations become scarce would protect forage fish and their predators from collapse with little effect on long-term average catches.

  20. Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway di Petshop "PetZone"

    Directory of Open Access Journals (Sweden)

    Siska Fadhilah Wati

    2013-09-01

    Full Text Available Dewasa ini teknologi informasi sangat membantu dalam dunia bisnis. Kemudahan yang ditawarkan akan menjaring lebih banyak konsumen. Namun, perusahan tingkat menengah seperti PetZone saat ini masih jarang yang menawarkan kemudahan berbelanja atau pemesanan layanan secara online. Oleh karena itu diperlukan sebuah sistem berbasis web di perusahaan PetZone yang dapat memudahkan pemilik dan karyawan dalam apenjualan, pelayanan, dan pemasaran, serta memudahkan pelanggan dalam jual-beli barang dan jasa. Atas dasar masalah tersebut dibangun Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway untuk menunjang proses bisnis yang ada. Sistem informasi dibangun menggunakan bahasa pemrograman PHP framework Codeigniter, javascript untuk tampilan yang dinamis, dan database MySQL. Proses pembuatan dan pengembangan Sistem Informasi Penjualan ini menggunakan metode air terjun. Metode air terjun meliputi kebutuhan pengguna, analisis, rancangan, implementasi dan pengujian. Pemodelan Sistem Informasi yang dibangun menggunakan metode berorientasi objek UML (Unified Modeling Language yang terdiri dari Use case  diagram, Class diagram dan Sequence diagram. Hasil pengujian Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway menunjukkan bahwa semua fitur yang terdapat baik dalam sistem informasi maupun SMS gateway dapat bekerja dengan baikdengan ratusan sample data, dan server SMS gateway dapat memproses lebih dari satu SMS secara bersamaan. Dalam perkembangan ke depannya nanti, Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway masih dapat dikembangkan lagi dengan menambah fitur-fitur pada SMS gateway sehingga lebih memudahkan konsumen.

  1. PERANCANGAN DAN PENGEMBANGAN PROTOTYPE SISTEM PARKIR

    Directory of Open Access Journals (Sweden)

    Indah Ayu Septriyaningrum

    2016-10-01

    Abstrak  Sistem parkir manual pada pelayanan parkir mall memerlukan pengelolaan dan pengembangan sistem yang lebih rumit dan jauh dari kata efisien. Informasi yang didapatkan pengelola parkir terkait kondisi parkir di lapangan setiap harinya masih kurang. Tujuan dari penelitian ini adalah merancang dan mengembangkan prototype sistem parkir. Metode pengembangan dan perancangan sistem pada penelitian ini adalah metode Waterfall. Hasil yang didapatkan adalah sistem ini dapat mendeteksi keberadaan mobil pada slot parkir yang tersedia dengan bantuan Intel Galileo Board Gen 2 sebagai kontroler, sensor LDR (Light Dependent Resistor dan sensor ultrasonik (PING sebagai alat bantu pendeteksi keberadaan mobil. Sistem dapat menampilkan aktifitas parkir pada aplikasi web seperti kondisi lahan parkir kosong, lahan terisi, waktu masuk, waktu keluar, lama parkir dan kapasitas parkir yang tersedia serta grafik pemakaian lahan parkir. Dapat disimpulkan bahwa perancangan dan pengembangan sistem berhasil diimplementasikan. Kata Kunci : Sistem parkir, waterfall, Intel Galileo Board Gen 2,sensor LDR (Light Dependent Resistor, sensor ultrasonik

  2. Sport Fishing Regulations

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The regulations for sport fishing on St. Vincent National Wildlife Refuge are outlined in this document. Fishing is only permitted from sunrise to sunset, and only...

  3. Fish Springs pond snail

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Communication scenario between the branch of Listing and Recovery, Fish and Wildlife Enhancement, and Fish Springs National Wildlife Refuge (NWR), in regards to the...

  4. Fish tapeworm infection

    Science.gov (United States)

    Fish tapeworm infection is an intestinal infection with the tapeworm parasite found in fish. ... The fish tapeworm ( Diphyllobothrium latum ) is the largest parasite that infects humans. Humans become infected when they eat raw ...

  5. Got a Sick Fish?

    Science.gov (United States)

    ... Welfare Veterinary Careers Public Health Got a sick fish? Fish with disease can show a variety of signs. If you notice your pet fish having any unusual disease signs, contact your veterinarian ...

  6. KEBIJAKAN PENGOPERASIAN BUBU DENGAN ALAT BANTU TERUMBU KARANG BUATAN DAN RUMPON DI WILAYAH REHABILITASI PERAIRAN KEPULAUAN SERIBU

    Directory of Open Access Journals (Sweden)

    Isa Nagib Edrus

    2014-05-01

    Full Text Available Ujicoba bubu bersama terumbu karang buatan dan rumpon di wilayah rehabilitasi perairan Pulau Pari dan Pramuka adalah untuk menentukan kesesuaian paket teknologi alat tangkap dan alat bantu dalam usaha perikanan yang layak dari sisi teknis, sosial dan ekonomi. Tulisan ini merupakan sintesa kebijakan pola pemanfaatan bubu dengan dua alat bantu pengumpul ikan. Pendekatan yang digunakan adalah analisis kebijakan dengan memformulasikan semua informasi yang relevan dan hasil penelitian terkait. Hasil analisis menunjukkan bahwa penggunaan bubu dalam perikanan multi alat tangkap dengan pemanfaatan alat bantu penangkapan tergolong efektif secara teknis, sosial dan ekonomis. CPUE bubu dari 4 hari perendaman/trip adalah ratarata 1,1 kg/unit/trip untuk nelayan Pulau Pari yang mengoperasikan bubu di dekat terumbu buatan dan 2,4 kg/unit/trip untuk nelayan Pulau Pulau Pramuka yang menaruh bubu di bawah rumpon. Perikanan bubu tergolong layak ekonomi jika terintegrasi dengan perikanan multi alat tangkap. Nilai tambah pendapatan dari penggunaan bubu berkisar pada Rp. 196.000–Rp. 400.000 per trip. Perikanan bubu memiliki prospek yang baik untuk dikembangkan dalam skala besar melalui diversifikasi usaha perikanan di wilayah perairan yang direhabilitasi dan aplikasinya dapat diterima nelayan. Alat bantu rumpon memberikan pengaruh lebih besar pada hasil tangkap bubu dibanding hanya penggunaan karang buatan.   The pot application by using artificial reefs and payaos as rehabilited fishing grounds in the waters of Pari and Pramuka Islands was to determine technological, social and economical feasible fisheries used pots and the both of fish aggregation divices (FAD. This paper is policy making of pot utility design using two kinds of the FAD. Policy analysis is an approach used to formulate any relevant information and related study results. The analysis showed that pot applications in multi gears fisheries using FAD were effective by technical, social and

  7. KUALITAS PRODUK DAN KUALITAS LAYANAN TERHADAP KEPUASAN DAN LOYALITAS NASABAH

    Directory of Open Access Journals (Sweden)

    Kharisma Nawang Sigit

    2017-05-01

    Full Text Available This study described the effect of product quality and service quality on sustomer satisfaction and loyalty. The population in this study was the customers of Kredit Usaha Rakyat at BRI Warungasem. The sampling method used in this research was purposive sampling. The samples in this study were 100 customers in People’s Business Credit (Kredit Usaha Rakyat BRI Batang Branch Unit Warungasem who had already taken at least two time KUR credits. The instrument of answers measurement used was a Likert scale 1-7 alternative answers. Testing instrument used was validity and reliability, and testing the model used was the coefficient of determination and F test (goodness of fit. Testing the hypothesis in this study used t test. Variables used in the study were product quality, service quality, customer satisfaction and loyalty.The results showed that there was the effect on the product quality and customer satisfaction. Service quality affected the customer satisfaction. Product quality affect the customer loyalty. The quality of service had an effect on customer loyalty. Customer satisfaction had an effect on customer loyalty. Penelitian ini menjelaskan pengaruh kualitas produk dan kualitas layanan terhadap kepuasan nasabah dan loyalitas. Populasi pada penelitian ini adalah nasabah Kredit Usaha Rakyat BRI Unit Warungasem. Metode pengambilan sampel yang digunakan dalam penelitian ini adalah purposive sampling. Sampel dalam penelitian ini adalah 100 nasabah Kredit Usaha Rakyat di BRI Cabang Batang Unit Warungasem yang sudah mengambil minimal dua kali kredit KUR.Pengukuran jawaban instrumen menggunakan skala likert 1-7alternatif jawaban. Pengujian instrumen menggunakan validitas dan reliabilitas, pengujian model menggunakan koefisien determinasi, dan uji F (goodness of fit. Pengujian hipotesis dalam penelitian ini menggunakan uji t. Variabel yang digunakan dalam penelitian yaitu Kualitas Produk, kualitas layanan, kepuasan dan loyalitas nasabah. Hasil

  8. GRIYA DAN OMAH Penelusuran Makna dan Signifikasi di Arsitektur Jawa

    Directory of Open Access Journals (Sweden)

    Josef Prijotomo

    1999-01-01

    Full Text Available Terms and names in architecture may sometimes be powerful in exploring history of ideas. Philology and hermeneutics are discipline of knowledge that utilize this notion in a very throgh and critical way. Inspired by those discipline of knowledge, this musing on `Griya' and `Omah' tries to demonstrate that their meaning is not `house' as many still understand them. Rather, both terms understood by the Jawanese as `any building' constructed. Critical assessment upon old documents named `Kawruh Griya' not only supportive for this understanding among the Javanese, but also provide us with a hint on one of ideals of `what a home is' among the Jawanese. The Javanese once had a notion that to dwell in a house is like sheltering under a shady tree. Since `griya' and `omah' may open our musing up to regions and cultures outside Jawa, this paper will limit its musing within the Jawanese. Abstract in Bahasa Indonesia : Kata, sebutan dan istilah yang digunakan dalam arsitektur terkadang memiliki kemampuan untuk membantu dan menjadi bahan kajian sejarah bagi dunia arsitektur. Kajian filologi dan hermeneutika dengan baik telah memanfaatkan kemampuan tersebut. Tanpa harus memaksakan diri untuk mengikuti dengan ketat bidang pengetahuan tersebut, penelusuran atas arti `griya' dan `omah' ini mencoba untuk mengungkap sebagian kecil dari sejarah arsitektur Jawa. Melalui interpretasi-menerangkan (Poespoprodjo 1987: 194-195 atas arti kedua kata tersebut di dalam berbagai naskah Kawruh Griya, keduanya samasekali tidak memiliki arti: `rumah'. Dengan penelusuran ini ditemukan pula petunjuk yang mengarah pada salah satu gagasan orang Jawa tentang rumah yakni `bagaikan berteduh di bawah pohon'. Meskipun kedua kata tersebut dapat membawa penelusuran ini ke wilayah di luar arsitektur dan masyarakat Jawa, harus diakui bahwa penelusuran ini masih dibatasi pada telusuran internal bahasa Jawa, yakni hanya mengkaji kedua kata tersebut di dalam perjalanan kebahasaan bahasa Jawa

  9. Pengujian Kemampuan Daya Samak Cube Black dan Limbah Cair Gambir Terhadap Mutu Kulit Tersamak

    Directory of Open Access Journals (Sweden)

    Gustri Yeni

    2016-06-01

    Full Text Available Leather tanning industries generally use sintetic tanner such as chrome (Cr+3 which can damage environment. Therefore, it is needed to find environmentally friendly tannery material, one is by using gambier. The aim of this research was to know tannic ability of gambier on goat’s leather and tuna fish. The gambier for tannery material contained high tannin (>70% namely Cube black and liquid waste from gambier processing. Concentration of tannery material that would be used for tanned was 1 kg. Tanned leather was tested to tannery degree (DP and physical test included pull strength, elongation, and rip strength, at same treatment compared to chrome tannery. Test results leather tanned showed that, the higher the concentration of tannery material the higher the results of DP, and the physical characteristic was better. Tanned leather from goat that used Cube black gambier concentration at 4% gave DP value approximately equal to chrome tanner (38,45% and 36,60%. For tanned tuna fish gave value of DP were 39,57% and 31,35%. Tannery material of gambier gave value of pull strength, rip strength, and elongation were higher than chrome’s. The value of pull strength of goat’s leather was 730,37 kg cm-2, tuna fish was 353,33 kg cm-2 got from liquid waste tanner. The value of rip strength fof goat’s leather was 353,33 kg cm-2, tuna fish skin 29,96 kg cm-2, and elongation value from tuna fish skin 202,0% was gotten from Cube black gambier. The result of the research showed that tannery material of gambier had tannery characteristic that can replace tanner of chrome.ABSTRAKIndustri penyamakan kulit umumnya menggunakan bahan penyamak sintetis seperti krom (Cr+3 yang dapat merusak lingkungan. Untuk itu, perlu dicari bahan penyamak yang ramah lingkungan, diantaranya menggunakan gambir. Tujuan penelitian ini adalah mengetahui daya samak gambir terhadap kulit kambing dan ikan tuna. Gambir untuk  bahan penyamak mengandung kadar tanin tinggi (>70% adalah

  10. RAGAM GENOTIPE IKAN TENGADAK, Barbonymus schwanenfeldii (Bleeker 1854 PERSILANGAN POPULASI JAWA DAN KALIMANTAN BERDASARKAN RAPD

    Directory of Open Access Journals (Sweden)

    Deni Radona

    2016-12-01

    Full Text Available Dalam rangka pengelolaan sumber genetik untuk pengembangan budidaya ikan tengadak maka perlu dilakukan evaluasi sumber genetik ikan tengadak asal Jawa dan Kalimantan, serta progeni hibridanya. Analisis genetik dilakukan secara molekuler dengan metode RAPD. Jumlah sampel yang digunakan untuk analisis RAPD sebanyak 10 ekor setiap populasi. Spesimen yang digunakan untuk analisis RAPD adalah sirip untuk induk dan whole body untuk hibrida. Hasil menunjukkan polimorfisme (32,43% dan heterozigositas (0,13 tertinggi terdapat pada ikan tengadak hasil persilangan betina Jawa x jantan Kalimantan, sedangkan yang terendah diperoleh pada persilangan betina Kalimantan x jantan Jawa (polimorfisme: 21,62% dan heterozigositas: 0,10. Berdasarkan dendrogram hubungan kekerabatan interpopulasi ikan tengadak hasil persilangan (betina Kalimantan x jantan Jawa dengan induknya (populasi Jawa dan Kalimantan menggunakan tiga primer RAPD (OPA-08, OPA-09, dan OPC-02 menunjukkan jarak genetik berkisar 0,48. Ikan tengadak betina asal Jawa dan jantan asal Kalimantan potensial meningkatkan keragaman genetik. In order to maintain the genetic sources of tinfoil barb for aquaculture development, it is necessary to evaluate the genetic diversity crossbred results of tinfoil barb from Java and Kalimantan. The genetic assessment was conducted by genotype trails using RAPD methods. The samples used for the analysis of RAPD was as much as 10 individuals. Specimens used for RAPD analysis was a fin for broodstock and whole body for the hybrid fish. The result showed that the highest polymorphism and heterozygosity were found 32.43% and 0.13 in population crossbred of female Java x male Kalimantan. While the lowest polymorphism and heterozygosity were detected on population of f Kalimantan x m  Java (21.62% and 0.10, respectively. Based on the relationship between tinfoil barb hybrid (female Kalimantan x male Java with a both broodstock population using three RAPD primers (OPA-08, OPA-09

  11. Lõuka tuuliku restaureerimine / Dan Lukas

    Index Scriptorium Estoniae

    Lukas, Dan

    2005-01-01

    Hiiumaal Pühalepa vallas Vahtrepa külas Lõuka talu maadel asuva pukktuuliku ajaloost, restaureerimise käigust. Teostus: Dan Lukas. Muinsuskaitseline järelevalve: Tõnu Sepp, tema kommentaar. Ill.:pukktuuliku lõiked ja korruste plaanid, 7 värv. fotot

  12. Corner Office: Google's Dan Clancy

    Science.gov (United States)

    Albanese, Andrew Richard; Oder, Norman

    2009-01-01

    This article presents an interview with Dan Clancy, engineering director for Google Book Search. In this interview, Clancy talks about the pending Google Book Search settlement, involving millions of volumes digitized from libraries, which drew a lawsuit from the Association of American Publishers and the Authors Guild. He also discusses pricing,…

  13. Corner Office: Google's Dan Clancy

    Science.gov (United States)

    Albanese, Andrew Richard; Oder, Norman

    2009-01-01

    This article presents an interview with Dan Clancy, engineering director for Google Book Search. In this interview, Clancy talks about the pending Google Book Search settlement, involving millions of volumes digitized from libraries, which drew a lawsuit from the Association of American Publishers and the Authors Guild. He also discusses pricing,…

  14. Fish mycobacteriosis (Tuberculosis)

    Science.gov (United States)

    Parisot, T.J.; Wood, J.W.

    1959-01-01

    The etiologic agent for the bacterial disease, "fish tuberculosis" (more correctly "mycobacteriosis"), was first observed in carp in 189& from a pond in France. Subsequently similar agents have been isolated from or observed in fish in fresh water, salt water, and brackish water, in fish in aquaria, hatcheries, and natural habitat~ (wild populations of fish). The disease has been recognized as an important infection among hatchery reared salmonid fishes on the West Coast of the United States, and in aquarium fishes such as the neon tetra, the Siamese fighting fish, and in salt water fish held in zoological displays.

  15. HUKUM DAN PERUBAHAN MASYARAKAT

    Directory of Open Access Journals (Sweden)

    Antoni Antoni

    2015-05-01

    Full Text Available Abstract: Where there is sugar there must be an ant, or likefish and water, as it were appropriate parable likens the Lawand Society, because where there is society there must be lawswill be needed. Law and society are two sides of which aredifficult to separate, because they will need each other andinfluence each other.ملخص:حیث یوجد السكر یجب أن یكون ھناك نملة، أو مثل السمك والماء، كما انھاكانت مناسبة المثل یشبھ القانون والمجتمع، وذلك لأن عندما یكون ھناك مجتمع یجبأن یكون ھناك وستكون ھناك حاجة القوانین .القانون والمجتمع وجھان لوالتي یصعبفصل، لأنھا سوف نحتاج إلى بعضنا البعض وتؤثر على بعضھا البعض.Kata Kunci: hukum dan perubahan masyarakat

  16. HASIL TANGKAPAN SAMPINGAN PADA PUKAT UDANG DAN ALTERNATIF PEMANFAATANNYA DI LAUT ARAFURA

    Directory of Open Access Journals (Sweden)

    Bambang Sumiono

    2012-11-01

    menjadi bahan pertimbangan dalam kebijakan pengelolaan untuk pemanfaatan HTS yang melimpah dan belum dimanfaatkan optimal. Tulisan ini membahas secara ringkas tentang densitas dan komposisi jenis ikan, daerah penyebaran, rasio HTS terhadap udang serta beberapa saran upaya pemanfaatan HTS untuk kepentingan industri perikanan. Commercially shrimp fishery in the Arafura Sea exploits a large amount of by-catch fishing composed mostly of demersal fish. In recent years, the small size of finfish and non edible crabs are found in large quantities in certain areas, meanwhile large finfish are rarely caught. Based on average value by sub areas of bycatch to shrimps, the average of ratio fish to shrimp in the Arafura Sea was 12 : 1. By-catch is remained the main issues of shrimp fishery management in the Arafura Sea. This is because of the by-catch was mostly discarded to the sea and only small portion of that by-catch was utilized by vessel’s crew. This phenomenon was ironic due to the by-catch was potentially suitable for human food consumption or processed to be animal feed. Management policies for improving the utilization of by-catch are still needed. Resource abundance and catch composition, ratio of shrimp and fish, and some recommendations for management option as a possible solution to the problem of utilization of shrimp by-catch in the Arafura Sea for fishing industry were discussed in this paper

  17. No Fishing Now,More Fish Later

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Fishing ban for ecological purposes starts on the Pearl River Since April1,a two-month ban on fishing has been imposed on the Pearl River valley in south China.It is the first fishing ban in this area with the purpose of preserving biodiversity in China’s third longest

  18. Microbiological spoilage of fish and fish products

    DEFF Research Database (Denmark)

    Gram, Lone; Huss, Hans Henrik

    1996-01-01

    Spoilage of fresh and lightly preserved fish products is caused by microbial action. This paper reviews the current knowledge in terms of the microbiology of fish and fish products with particular emphasis on identification of specific spoilage bacteria and the qualitative and quantitative...

  19. Gangguan Fungsi Penghidu dan Pemeriksaannya

    Directory of Open Access Journals (Sweden)

    Effy Huriyati

    2014-01-01

    Full Text Available AbstrakLatar belakang: Fungsi penghidu pada manusia memegang peranan penting. Gangguan penghidu dapat mempengaruhi keselamatan dan kualitas hidup seseorang. Tujuan: Untuk mengetahui jenis gangguan penghidu, penyebab gangguan penghidu, dan pemeriksaannya. Tinjauan Pustaka: Gangguan penghidu dapat berupa anosmia yaitu hilangnya kemampuan penghidu, atau hiposmia yaitu berkurangnya kemampuan penghidu. Gangguan penghidu disebabkan gangguan konduksi, gangguan sensoria dan gangguan neural. Penyakit tersering penyebab gangguan penghidu yaitu rinosinusitis kronis, rinitis alergi, infeksi saluran nafas atas dan trauma kepala. Ada beberapa modalitas pemeriksaan kemosensoris fungsi penghidu diantaranya Tes “Sniffin sticks”. Dengan tes „Sniffin sticks” dapat diketahui ambang penghidu, diskriminasi penghidu dan identifikasi penghidu seseorang. Kesimpulan: Gangguan penghidu memerlukan perhatian khusus. Diantara beberapa modalitas pemeriksaan kemosensoris penghidu, tes “Sniffin sticks” mempunyai beberapa kelebihan.Kata kunci: Gangguan penghidu, anosmia, hiposmia, tes “Sniffin sticks”.AbstractBackground: Olfactory function in humans plays an important role. Olfactory disorders can affect the safety and quality of life. Objective: To determine the type of olfactory disorder, the causes of olfactory disorders, and the examination. Literature Review: Olfactory disorder can be not smell anything or anosmia, and reduced of smell or hyposmia. Olfactory disorders caused by conduction disturbances, neural disturbances and sensoris disturbances. Disease that often causes disturbances of olfactory function is, chronic rhinosinusitis, allergic rhinitis, upper respiratory tract infections and head trauma. There are several modalities to examine chemosensoris smelling function, one of them is “Sniffin sticks” test. This test can examine threshold, discrimination, and identification of smelling. Conclusions: Impaired smelling require special attention

  20. PEMBINAAN DAN PENGEMBANGAN USAHA KECIL

    Directory of Open Access Journals (Sweden)

    Sentot Harman Glendoh

    2001-01-01

    Full Text Available Small business as an economic activity of society on a small scale, has a central role in the economy of Indonesia. Although the economic crisis has created a great disturbance in the live of large and middle-size business, apparently small business continues to function well in the lower levels of the economy. The main role of small business is: 1 use excess labor; 2 as a producer of goods and services at reachable prices for the lower economic levels of society; 3 as a potential producer of foreign exchange because of the success of this type of industry in producing non-oil commodities for export. Remembering that the role of small business is large and has great influence on the lower economic levels of society, it is quite necessary to pay attention to the development of this sector. Abstract in Bahasa Indonesia : Usaha kecil sebagai kegiatan ekonomi rakyat berskala kecil memiliki peran sentral dalam perekonomian Indonesia. Walaupun krisis ekonomi telah memporakporandakan kehidupan bidang usaha besar dan menengah, ternyata usaha kecil tetap tegar dan berjalan marak dikawasan kehidupan ekonomi tingkat bawah. Peran pokok usaha kecil ini adalah: (1 sebagai penyerap tenaga kerja, (2 sebagai penghasil barang dan jasa pada tingkat harga yang terjangkau bagi kebutuhan rakyat banyak yang berpenghasilan rendah, (3 sebagai penghasil devisa negara yang potensial kerena keberhasilannya dalam meproduksi komoditi ekspor non migas. Mengingat peran usaha kecil tersebut sangat besar andilnya bagi negara dan masyarakat kecil dilapisan bawah, maka pembinaan dan pengembangannya sangat perlu diperhatikan. Kata kunci: pembinaan, pengembangan, usaha kecil.

  1. BIOMASA, PRODUKTIVITAS KAPAL PENANGKAP DAN POTENSI PRODUKSI IKAN DEMERSAL DI LAUT ARAFURA

    Directory of Open Access Journals (Sweden)

    Purwanto Purwanto

    2015-09-01

    Full Text Available Tulisan ini menyajikan hasil kajian potensi produksi dan upaya penangkapan optimal serta menjelaskan perkembangan perikanan demersal di Laut Arafura, termasuk pula estimasi dampak peningkatan upaya penangkapan terhadap produktivitas kapal dan hasil tangkapan ikan demersal. Kajian menggunakan data statistik produksi perikanan yang dikoreksi dengan menambahkan estimasi hasil tangkapan yang tidak tercatat dan data jumlah kapal penangkap dengan memperhitungkan pula estimasi jumlah kapal yang beroperasi secara ilegal. Berdasarkan hasil analisis, potensi produksi ikan demersal yang dapat dihasilkan secara lestari dari pemanfaatan stok ikan demersal di Laut Arafura adalah 539 ribu ton/tahun dengan upaya penangkapan sekitar 903 unit kapal pukat ikan 180 GT. Walaupun upaya penangkapan dari kapal yang memiliki surat izin penangkapan ikan di Laut Arafura tahun 2011 lebih rendah dibandingkan upaya penangkapan optimal untuk menghasilkan MSY, stok ikan demersal tersebut telah dimanfaatkan melebihi tingkat optimumnya akibat tingginya intensitas operasi kapal perikanan tanpa izin. Estimasi kerugian hasil tangkapan akibat kegiatan penangkapan ikan ilegal juga disajikan disini. This paper presents result of the assessment of potential production and optimal fishing effort, and briefly describes the past development of the demersal fishery in the Arafura Sea, including the estimated impact of increased fishing effort on the productivity of vessels and demersal fish catches. The assessment used corrected fisheries statistic data taking into account unreported catches and corrected fishing vessel data taking into account vessels operated illegally. Based on the analysis, the production potential of demersal fish that can be produced in a sustainable manner of demersal fish stocks in the Arafura Sea is 539 thousand tons/year to attempt the arrest of about 903 units of fishing trawlers 180 GT. Although fishing effort from vessels granted license in

  2. KINERJA DAN EFISIENSI BANK PEMERINTAH (BUMN DAN BUSN YANG GO PUBLIK DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Sugeng Haryanto

    2012-06-01

    Full Text Available Penelitian ini menganalisis kinerja dan tingkat efisiensi bank-bank BUMN dan BUSN yang go Publik di Bursa Efek Indonesia.  Sample penelitian ini mengambil  tiga bank BUMN Bank BNI 46, Bank Mandiri dan Bank BRI  dan tiga bank BUSN (Bank BCA, Bank Niaga dan Bank Panin dengan periode analisis tahun 2005-2011. Varibael yang digunakan meliputi ROA, ROE, LAR. LDR, NPL dan BOPO. Tujuan dari penelitian ini adalah melihat dan menganalisis perbedaan kinerja antara Bank BUMN dan BUSN yang go public di Bursa Efek Indonesia tahun 2005-2011.  Pendekatan pengukuran kinerja yang digunakan adalah Return on Asset (ROA, Return on Equity (ROE dan Loan to Deposit Ratio (LDR, Loan to Asset Ratio (LAR,  dan efisiensi bank. Hasil dari penelitian menunjukkan bahwa 1 Bank-bank nasional, baik itu bank BUMN maupun BUSN menunjukkan kinerja yang semakin baik, 2 tidak terdapat perbedaan yang signifikan antara kinerja bank BUMN dan BUSN untuk variabel ROA, ROE, LAR, LDR, dan BOPO sedangkan variabel NPL yang merupakan indikator risiko kredit menunjukkan adanya perbedaan yang signifikan antara Bank BUMN dan BUSN

  3. AGROFORESTRI UNTUK ADAPTASI DAN MITIGASI PERUBAHAN IKLIM

    Directory of Open Access Journals (Sweden)

    Tigor Butarbutar

    2017-01-01

    Full Text Available Hilangnya tutupan lahan hutan karena konversi hutan untuk pemukiman, perkebunan, pertanian dan kebutuhan untuk pembangunan di sektor lain, telah menyebabkan perubahan pola cuaca/iklim di berbagai tempat. Perubahan iklim dapat diantisipasi dengan mitigasi dan adaptasi. Mitigasi berarti usahausaha pencegahan yang perlu dilakukan, sedangkan adaptasi merupakan kegiatan-kegiatan penyesuaian yang perlu dilakukan untuk dapat hidup dan bertahan dan meningkatkan ketahanan, kelenturan dan mengarahkemigrasikarenakondisiiklimyangberbeda.Agroforestridapatmemitigasidanmengadaptasi perubahaniklim dengan alasan-alasansebagaiberikut: aPencampuranjenis pohonpenghasilkayu,buah dan lain-lain, karena campuran jenis lebih baik dari tanaman murni; b Pencampuran jenis yang didasarkan pada sifat toleransi ( dan , sehingga akan memanfaatkan seluruh cahaya untuk fotosintesis; c Pencampuran perbedaan umur; d Pencampuran berdasarkan perbedaan waktu pemanenan; e Penggabungan nilai ekonomi, sosial dan budaya sehingga perubahan vegetasi dapat berjalan seiring dengan perubahan sosial dan budaya secara berangsur yang dapat disesuaikan dengan perubahan iklim; dan f Dapat digunakan sebagai model untuk memfasilitasi perubahan kelompok vegetasi menjadi kelompok yang baru (adaptasi, seperti teori perubahan vegetasi melalui perladangan berpindah-pindahyangteratur.

  4. ANALISIS KANDUNGAN GIZI DAN BAU LUMPUR IKAN BANDENG (Chanos chanos DARI DUA LOKASI YANG BERBEDA

    Directory of Open Access Journals (Sweden)

    H Hafiluddin

    2014-04-01

    Full Text Available Bandeng (Chanos chanos merupakan salah satu komoditas yang strategis untuk memenuhi kebutuhan protein yang relatif murah dan digemari oleh konsumen di Indonesia.Bau lumpur pada ikan umumnya pada ikan tawar disebabkan senyawa kimia yaitu 2-Methylisoborneol dan Geosmin.Tujuan untuk mengetahui kandungan kandungan gizi dan geosmin ikan bandeng (Chanos chanos dari dua lokasi yang berbeda yaitu perairan tambak Lamongan dan Pamekasan.Penelitian ini menggunakan metode Uji Test-T dengan menggunakan dua faktor untuk analisa uji proksimat. Kandungan gizi ikan bandeng bervariasi, yaitu kadar air ikan bandeng Lamongan 74,71-76,06% dan Pamekasan 68,50-73,34%, protein ikan bandeng Lamongan 14,84-19,47 dan Pamekasan 21,29-23,53%.Bau lumpur ikan bandeng Lamongan mempunyai rata-rata 2,848 (sedikit berbau lumpur dan Pamekasan 4,303 (sedikit sekali berbau lumpur.Kata Kunci: ikan bandeng, kandungan gizi, bau lumpurANALYSIS ON THE NUTRITION AND MUD SMELL OF MILKFISH (Chanos chanos FROM TWO DIFFERENT LOCATIONSABSTRACTMilkfish (Chanos chanos is one of strategic commodities for the fulfillment of protein needs that is relatively cheap and favored by consumers in Indonesia. The mud smell from the fish, usually freshwater fish, is caused by chemical compounds, namely 2-Methylisoborneol and Geosmin. The objective of this research was to find out the nutrition and geosmin of milkfishes (Chanos chanos that were from two different locations, namely Lamongan and Pamekasan fish ponds. This research used testing method T-Test with two factors for proximate test analysis. The nutrition consisted in a milkfish varies, in which the water content contained in Lamongan milkfish was 74.71-76.06% whereas Pamekasan milkfish was 68.50-73.34% and the protein contained in Lamongan milkfish was 14.84-19.47 whereas Pamekasan milkfish was 21.29-23.53%. The mud smell of Lamongan milkfish was in average 2.848 (a bit mud smelling whereas Pamekasan milkfish was 4.303 (hardly mud smelling

  5. INDUSTRIALISASI DAN TANTANGANNYA PADA SEKTOR PENDIDIKAN

    Directory of Open Access Journals (Sweden)

    Arif Unwanullah

    2015-10-01

    Full Text Available Abstrak: Industrialisasi dan Tantangannya pada Sektor Pendidikan. Sumbangan pendidikan dalam perubahan dan pembangunan masa lalu telah bergeser dengan kemajuan teknoekonomi dan komunikasi. Perubahan yang terjadi telah menggeser tatanan kehidupan dan pandangan masyarakat. Materialisme, kapitalisme, efisiensi, dan efektivitas telah menjadi tujuan dan semangat hidup. Pergeseran pandangan masyarakat telah mengubah pula pandangan keberhasilan dan mutu pendidikan, di mana pendidikan diukur dari keberhasilan dalam keterserapan lulusan dalam dunia kerja, oleh karenanya pendidikan dianalisis dari karakteristik sebagai investasi (capital-investment. Pergeseran makna dan tanggung jawab pendidikan mendorong dunia pendidikan melakukan pembaruan dengan alternatif: membangun pembaruan penalaran warganya menuju pemerdekaan dan pendewasaan, pendidikan dilaksanakan secara komprehensif dan bekerjasama dengan semua pihak secara kemitraan, dan membangun visi pendidikan secara komprehensif dan simultan dengan semua pihak. Kata kunci: perubahan sosial, materialistik, modernisasi dan kapitalis Abstract: Industrialization and its education Sector challenges. Contribution of education has shifted with the progress of technology, economy, and communication. The changes have shifted the society's views. Materialism, capitalism, efficiency, and effectiveness have become of interest and enthusiasm for life. The shift has changed society's view, i.e. the view of success and quality of education, in which education is measured from a rate of the absorption of graduates into labor market, therefore education is analyzed from the characteristics of an investment (capital-investment. A shift in meaning and responsibility of education encourages the education sector to create reformation through these following alternatives: creating new thoughts towards liberation and maturation, implementing education comprehensively and cooperating with all parties in partnership, and creating

  6. Fundamentals of fluorescence and fluorescence microscopy.

    Science.gov (United States)

    Wolf, David E

    2013-01-01

    This chapter discusses the fundamental physics of fluorescence. The application of fluorescence to microscopy represents an important transition in the development of microscopy, particularly as it applies to biology. It enables quantitating the amounts of specific molecules within a cell, determining whether molecules are complexing on a molecular level, measuring changes in ionic concentrations within cells and organelles, and measuring molecular dynamics. This chapter also discusses the issues important to quantitative measurement of fluorescence and focuses on four of quantitative measurements of fluorescence--boxcar-gated detection, streak cameras, photon correlation, and phase modulation. Although quantitative measurement presents many pitfalls to the beginner, it also presents significant opportunities to one skilled in the art. This chapter also examines how fluorescence is measured in the steady state and time domain and how fluorescence is applied in the modern epifluorescence microscope.

  7. Plastic fish

    CERN Multimedia

    Antonella Del Rosso

    2015-01-01

    In terms of weight, the plastic pollution in the world’s oceans is estimated to be around 300,000 tonnes. This plastic comes from both land-based and ocean-based sources. A lecture at CERN by chemist Wolfgang Trettnak addressed this issue and highlighted the role of art in raising people’s awareness.   Artwork by Wolfgang Trettnak. Packaging materials, consumer goods (shoes, kids’ toys, etc.), leftovers from fishing and aquaculture activities… our oceans and beaches are full of plastic litter. Most of the debris from beaches is plastic bottles. “PET bottles have high durability and stability,” explains Wolfgang Trettnak, a chemist by education and artist from Austria, who gave a lecture on this topic organised by the Staff Association at CERN on 26 May. “PET degrades very slowly and the estimated lifetime of a bottle is 450 years.” In addition to the beach litter accumulated from human use, rivers bring several ki...

  8. ISOLASI FRAGMEN TERTENTU GEN HORMON PERTUMBUHAN IKAN MAS MAJALAYA DAN NILA GIFT DENGAN METODE CTAB–PCR

    Directory of Open Access Journals (Sweden)

    Ibnu Dwi Buwono

    2016-11-01

    Full Text Available Hormon pertumbuhan (Growth Hormone/GH pada ikan berperan untuk memacu pertumbuhan, di samping terlibat juga dalam fungsi osmoregulasi, pengaturan keseimbangan cairan elektrolit, dan proses-proses metabolisme. Metode CTAB (Cetyl Trymethyl Ammonium Bromide digunakan untuk mengisolasi DNA sel ikan (DNA genomik dari sirip ekor ikan mas Majalaya dan nila GIFT. Isolasi gen hormon pertumbuhan dari DNA genomik kedua jenis ikan dapat disintesis dengan primer cGH (carp Growth Hormone dan TiGH (Tilapia Growth Hormone menggunakan metode PCR (Polymerase Chain Reaction. Hasil elektroforegram produk amplifikasi PCR untuk sebagian gen hormon pertumbuhan ikan mas Majalaya dapat dideteksi dengan ukuran fragmen DNA sebesar 615 bp dan 349 bp. Fragmen DNA hormon pertumbuhan ikan nila GIFT dapat dideteksi dengan ukuran 597 bp. The roles of growth hormones in fish are for growth promotion. They are also involved in osmoregulation function, balance regulation of liquid electrolite, and correlated with metabolism processes. CTAB (Cetyl Trymethyl Ammonium Bromide methods was used to isolate fish cell DNA (genomic DNA from caudal fin of Majalaya carp and GIFT nile. Growth hormone gene isolates from two kinds of genomic DNA can be synthesized with primers cGH (carp Growth Hormone and TiGH (Tilapia Growth Hormone using PCR (Polymerase Chain Reaction method. Electroforegram product of the PCR amplification for partial growth hormone gene of Majalaya carp fish can be detected with DNA fragment size of 615 bp and 349 bp. DNA fragment of growth hormone of GIFT nile tilapia can be detected with size of 597 bp.

  9. Kebutuhan Vitamin C Untuk Pertumbuhan dan Meningkatkan Ketahanan Benih Kerapu Macan (Epinephelus fuscoguttatus

    Directory of Open Access Journals (Sweden)

    Nyoman Adiasmara Giri

    2016-11-01

    Full Text Available Penelitian untuk mengetahui kebutuhan vitamin C untuk pertumbuhan dan ketahanan benih ikan kerapu macan (Epinephelus fuscoguttatus telah dilakukan, menggunakan 18 buah bak polikarbonat volume 100 liter yang dilengkapi dengan sistem air mengalir dan aerasi. Benih kerapu macan dengan bobot rata-rata 23,0 ± 0,1 gram ditebar dalam bak penelitian dengan kepadatan 12 ekor per bak. Ikan diberi pakan penelitian 2 kali sehari pada level satiasi selama 13 minggu. Pakan penelitian diformulasi dengan kandungan nutrien yang sama kecuali kandungan vitamin C. Pada formula pakan ditambahkan vitamin C (L-ascorbyl-2-phosphate Magnesium, APM dengan dosis berbeda, yaitu 0, 15, 30, 60, 120, dan 250 mg/kg pakan. Pakan dibuat dalam bentuk pelet dan dikeringkan menggunakan freeze dryer. Penelitian dirancang menggunakan rancangan acak lengkap (RAL dengan 6 perlakuan beda kandungan vitamin C dalam pakan dan setiap perlakuan terdiri atas 3 ulangan. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan tanpa penambahan vitamin C pertumbuhannya, kadar hemoglobin dan hematokrit darah, aktivitas fagositik, dan indeks fagositiknya adalah paling rendah dibandingkan dengan kelompok ikan lainnya. Dari hasil ini disimpulkan bahwa diperlukan penambahan vitamin C sebanyak 30 mg/kg pakan untuk pertumbuhan ikan yang baik dan penambahan vitamin C sebanyak 60—120 mg/kg pakan untuk kesehatan ikan kerapu macan. The experiment to determine dietary vitamin C requirement for good growth of tiger grouper has been conducted in 18 polycarbonate tanks, 100 liters volume. Each tank is equipped with flow-through water system. Twelve juvenile of tiger grouper (23.0 ± 0.1 gram in body weight, which were produced in hatchery, were randomly selected and stocked in each tank. Fish fed by experimental diets twice everyday at satiation level for 13 weeks. Experimental diets were formulated to contain the same level of nutrients (protein, lipid, and energy, except for vitamin C. Graded level of

  10. IMPLEMENTASI KEBIJAKAN PEMUNGUTAN PAJAK BUMI DAN BANGUNAN

    Directory of Open Access Journals (Sweden)

    Arifuddin - Sahabu

    2012-05-01

    Full Text Available ABSTRAKS   Penelitian ini menggunakan metode kualitatif dengan bentuk studi kasus. Pengumpulan data dilakukan dengan menggunakan teknik wawancara, dokumentasi, dan observasi. Analisis data mengunakan metode analisis Miles dan Huberman, dengan melalui tiga prosedur yaitu reduksi data, penyajian data dan menarik kesimpulan/verifikasi. Sedangkan keabsahan menggunakan teknik berdasarkan atas kriteria derajat kepercayaan, keteralihan, ketergantungan, dan kepastian suatu data. Tuntutan menghadapi  implementasi Otonomi Daerah mengandung arti pentingnya Pemerintah Daerah memperhatikan kemampuan “self suporting” dalam bidang keuangan. Sumber pendapatan daerah tidak hanya di peroleh dari Pendapatan Asli Daerah, tetapi juga berupa pemberian bagi hasil dari penerimaan Pemerintah Pusat. Diantara sumber penerimaan tersebut adalah Pajak Bumi dan Bangunan. Dalam Undang-undang Nomor 25 Tahun 1999 Tentang perimbangan keuangan antara Pemerintah Pusat  dan Daerah, diantaranya dijelaskan bahwa sumber penerimaan daerah dalam penyelenggaraan desentralisasi berasal dari dana perimbangan penerimaan Pajak Bumi dan Bangunan disamping pemberian Dana Alokasi Umum dan Alokasi Khusus. Langkah Implementasi dari pelaksanan pemungutan Pajak Bumi dan Bangunan tersebut dilakukan Departemen Keuangan melalui Kantor Pelayanan Pajak Bumi dan Bangunan, sedang Pemerintah daerah menerima pelimpahan penagihan pada Sektor Perkotaan dan Sektor Pedesaan. Meskipun telah ada pelimpahan kewenangan kepada daerah, akan tetapi pelimpahan kewenangan tersebut terbatas pada mekanisme penagihan saja, sedang implementor yang menyangkut masalah administrasi masih berada pada Kantor Pelayan Pajak Bumi dan Bangunan. Kondisi demikian ini ditambah dengan kurangnya koordinasi antara unit organisasi pelaksana menyebabkan setiap tahun terjadi tunggakan. Sebagai sandaran teoritik utama  untuk mendiskripsikan serta menganalisis hambatan-hambatan implementasi kebijakan publik (dalam hal ini pemungutan

  11. TRANSFORMASI ORGANISASIONAL DAN MSDM: HAMBATAN DAN IMPLIKASINYA PADA REKRUTMEN DAN SELEKSI

    Directory of Open Access Journals (Sweden)

    Licen Indahwati Darsono

    2002-01-01

    Full Text Available Rapid change of environment is an external force that causes the organization to transform. Fundamentally, the main purpose of the transformation is to change the organizational structure to be more flexible and competitive with fewer hierarchial levels, managers, and employees. The transformation needs to be radical, causing resistance from the organization's members. Their resistance can cause the transformation to fail, therefore organization must find ways to lessen this resistance. First, organization must learn about the diversity of its members' cultures and values. Second, organization must build its own organizational culture which can support the success of the transformation, by communicating it with the members of the organization. To support organizational culture building efforts, it needs changes in human resources practices, especially recruitment and selection. Abstract in Bahasa Indonesia : Perubahan yang cepat dalam lingkungan merupakan kekuatan eksternal yang mengakibatkan transformasi dalam sebuah organisasi. Pada dasarnya, tujuan utama dari transformasi tersebut adalah merubah struktur organisasi agar menjadi lebih fleksibel dan mampu bersaing, dengan tingkat structural yang sedikit, serta jumlah manajer dan karyawan yang lebih kecil. Transformasi tersebut harus menyeluruh, dan hal ini dapat menyebabkan resistensi dari para anggota organisasi yang memperhambat perubahan tersebut. Resistensi itu bisa menyebabkan perubahan tersebut batal, oleh karena itu organisasi harus mencari jalan untuk mengurangi hambatan-hambatan tersebut. Pertama, organisasi harus belajar keanekaragaman dari budaya dan nilai anggotanya. Kedua, organisasi harus mengembangkan budaya organisasi sendiri melalui komunikasi yang baik dengan anggotanya. Untuk mendukung usaha mengembangkan budaya organisasi, harus ada perubaban pada kebijakan sumber daya manusia, terutama dalam rekrutmen dan seleksi karyawan. Kata kunci: transformasi, resistensi, budaya

  12. VAKSIN DENGUE DAN PERKEMBANGANNYA SAAT INI DAN DI MASA MENDATANG

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    Triwibowo Ambar Garjito

    2012-09-01

    Full Text Available Dengue virus merupakan salah satu virus anggota dari famili Flaviviridae yang sejak tahun 1956 telah dikenal dapat menimbulkan demam dengue maupun demam berdarah dengue (DBD. Penyakit yang ditularkan oleh nyamuk Aedes aegypti ini diperkirakan telah menjangkiti pada selatar 50-100 juta manusia dengan 500.000 kasus di antaranya dalam manifestasi yang ganas yang dikenal sebagai dengue haemorrhagic fever dan dengue shock syndrome dan 25.000 di antaranya berakibat fatal (meninggal. Saat ini pengembangan vaksin merupakan salah satu solusi yang diharapkan dapat menekan penyebaran penyakit tersebut. E (envelope merupakan salah satu bagian dari protein struktural virus yang sangat penting dalam pengembangan vaksin, yaitu sebagai badan yang memproduksi antibodi netralisasi untuk protein. Non-struktural protein l juga telah diketahui sebagai salah satu komponen penting dalam pengembangan vaksin oleh karena kemampuannya untuk dapat diekspresi pada permukaan sel yang diinfeksi yang dapat menjadi target untuk immune cytolisis. Ada dua pendekatan yang digunakan dalam memproduksi suatu vaksin dengue, yaitu: a. Vaksin hidup yang telah dilemahkan (live attenuated vaccine: b. Vaksin hasil rekayasa (engineered vaccine. Penelitian terhadap vaksin DENV baik rekombinan maupun non-rekombinan yang didasarkan pada uji virus telah dilakukan secara terus-menerus baik pada monyet dan manusia. Sampai saat ini telah dikembangkan sejumlah kandidat vaksin DENV yang berdasar pada tetravalent virus dengue, yaitu a. vaksin konvensional, b. vaksin dengue rekombinan berdasar pada flavivirus, c. vaksin intertypic chimeric, d. vaksin chimerivac, e. vaksin dengue rekombinan menggunakan vector non-ftavivirus dan f. vector adenovirus. Namun demikian, sampai sekarang belum ada vaksin yang siap digunakan untuk menangkal infeksi ke empat serotype virus dengue, sehingga masih diharapkan untuk pengembangan virus lebih lanjut.   Kata kunci: Aedes aegypti, dengue virus, vaksin dengue.

  13. Muhammad sebagai pemimpin agama dan negara periode Makkah dan Madinah

    Directory of Open Access Journals (Sweden)

    Ummu Zakiyah Maulidah

    2014-07-01

    Full Text Available Muhammad was born in Mecca between AD 570 and 580 in the Banu Hashims,a reputable family of Quraysh. In Mecca, Muhammad is portrayed as a privatecitizen, as he had to limit himself and adapt his behavior to face the situationduring jahiliyyah era. While, in Medina he governed the community and guidedthem in religious life. His arrival in Medina has significant contribution towardhis role as political leader. His power in this city had protected himself and hisfollowers from violent opposition of the Quraysh. The doctrines he employedin politics were inseparable from the religious values rooted from the Quran.Through his innovation and skillful diplomacy, he had converted the peopleof Mecca and Medina into brighter Arab civilization. Such a positive changereflected his monumental accomplishment for the people.Muhammad lahir di kota Makkah antara tahun 570 sampai 580 Masehi dikeluarga Bani Hasyim, suku Quraisy terkemuka di Arab. Di kota Makkah,Muhammad dianggap sebagai masyarakat biasa, sebab ia harus membatasidirinya dan menyesuaikan prilakunya dalam menghadapi keadaan yangterjadi pada zaman jahiliyyah. Sedangkan di Madinah, ia dianggap sebagaipemimpin masyarakat dan menjadi pembimbing umat dalam hidup beragama.Kedatangannya di Madinah juga memberikan konstribusi besar pada perannyasebagai pemimpin politik. Kekuasaan di kota ini sangat membantu dirinya danpara pengikutnya dari kerasnya kelompok oposisi Quraisy. Doktin-doktrin yangdigunakan Muhammad dalam dunia politik juga tidak lepas dari nilai-nilaiagama yang bersumber dari al Quran. Dengan kreatifitas dan inovasinya dalammemimpin, Muhammad mampu mengarahkan masyarakat Makkah dan Madinah menuju kebangkitan peradaban bangsa Arab yang lebih cemerlang.Kondisi yang semakin membaik tersebut, tidak lain merupakan sebuah karyadan jasa monumental yang telah diukir oleh Muhammad untuk umatnya.

  14. ADAPTASI PERIKANAN PUKAT CINCIN DI LAUT JAWA DAN IMPLIKASINYA TERHADAP PENGELOLAAN

    Directory of Open Access Journals (Sweden)

    Suherman Banon Atmaja

    2014-11-01

    Full Text Available Usaha perikanan akan mengalami fluktuasi terkait dengan dinamika faktor alam, sedangkan nelayan mempunyai kemampuan yang lentur dan adaptif dalam kelangsungan usahanya. Tujuan utama makalah ini adalah membahas tentang kemampuan adaptasi perikanan purse seine untuk bertahan dari usaha perikanan tangkap. Pemetaan rekaman data sistem pemantauan kapal (VMS purse seine pelagis kecil tahun 2012 dilakukan berdasarkan kriteria kecepatan kapal nol adalah waktu melakukan aktifitas penangkapan. Daerah penangkapan perikanan pukat cincin pada saat ini telah menyebar semakin luas, tidak terbatas di wilayah teritorial dan perairan nusantara, tetapi sudah sampai ke wilayah Samudera (ZEEI. Jumlah hari laut pada perikanan cantrang kurang dari sebulan memperoleh rata – rata pendapatan nelayan ABK hampir dua kali lipat dari pada ABK pukat cincin yang beroperasi lebih dari sebulan. Rotasi penggunaan alat tangkap di Laut Jawa adalah suatu kondisi yang muncul ke permukaan sebagai sinyal pergeseran populasi ikan dari karakteristik sumber daya yang multi-spesies. Fenomena hasil tangkapan yang tidak tercatat dan dilaporkan menyebabkan pendugaan stok ikan dengan akurat yang rendah akan menimbulkan ketidakpastian dalam penyusunan rekomendasi. Tersedianya sistem pemantauan kapal perlu didukung oleh kegiatan validasi hasil tangkapan yang didaratkan, untuk itu sangat perlu meningkatkan keterlibatkan enumerator dan observer di atas kapal serta penguatan sistem log-book yang sedang berjalan.   The fishing business will fluctuate as a result of high connectivity with the dynamic and nature of environmental factors, in the other side, experience fishers have flexible and adaptive capabilities in order to sustain their business. The objective of this paper is to describe the purse seine fishery adaptability to survive from long term fishing. Plotting data on recorded vessel monitoring system (VMS of small pelagic purse seine in 2012 were applied based on the criteria of the

  15. KONTEKS DAN KONSTRUKSI SOSIAL MENGENAI KEMATIAN ELEKTIF ( EUTHANASIA ∗

    Directory of Open Access Journals (Sweden)

    Helly Prajitno Soetjipto

    2015-09-01

    konteks sosial dan konstruksi sosial kematian. Euthanasia didiskusikan di dalam suatu kerangka pikir yang mencoba memberi perhatian kepada hal-hal yang kontekstual dan interpretatif fenomena sosial suatu proses kematian dan kejadian kematian

  16. PERJANJIAN SEWA GUNA USAHA ANTARA LESSEE DAN LESSOR

    Directory of Open Access Journals (Sweden)

    Aprilianti Aprilianti

    2014-03-01

    Full Text Available Perjanjian sewa guna usaha (leasing yang diadakan oleh Lessor dan Lesseen dilakukan secara tertulis dalam bentuk perjanjian standar. Isi perjanjian tersebut ditentukan oleh jenis dari leasing itu sendiri dan hubungan hukum (hak dan kewajiban timbal balik antara Lessor dan Lessee. Bagi Lessor, hak dan kewajibannya adalah memperoleh pembayaran sebagai imbalan jasa dan menyerahkan barang modal kepada Lessee. Sedangkan hak dan kewajiban Lessee adalah meperoleh kegunaan dari barang modal dan membayar sewa secara berkala. Tidak dipenuhinya hak dan kewajiban masing-masing pihak maka dapat disebut wanprestasi. Perjanjian akan berakhir jika hak dan kewajiban Lessor dan Lessee telah dilaksanakan sesuai dengan perjanjian. Kata Kunci: Leasing, Lessor, Lessee, hak dan kewajiban.

  17. Pembuatan Dan Karakterisasi Papan Partikel Peredam Suara Dari Campuran Resin Poliester Dan Jerami Padi

    OpenAIRE

    Marjuki, Ismail

    2013-01-01

    060802033 Telah dilakukan penelitian papan partikel dari jerami padi yang direaksikan dengan polyester tak jenuh yukalac 157 BQTN ? EX menggunakan katalis mepoxe. Papan partikel ini dibuat dengan proses pencampuran dengan perbandingan antara poliester dan jerami padi adalah 30:70%; 35:65%; 40:60%; 45:55%; dan 50:50%, yang dilanjutkan dengan pengempaan. Karakterisasi dan analisa yang dilakukan menunjukkan bahwa sifat mekanik tegangan optimum pada campuran 50:50 antara polyester dan jerami p...

  18. KONSEP WANUA DAN PALILI DI KONFEDERASI AJATAPPARENG: SEJARAH DAN BUDAYA POLITIK ORANG BUGIS DI SULAWESI SELATAN

    OpenAIRE

    Abd. Latif

    2014-01-01

    Kajian ini akan mendiskusikan tentang konsep wanua dan palili dalam struktur kerajaan-kerajaan yang tergabung dalam Konfederasi Ajatappareng di Sulawesi Selatan. Wanua dan palili adalah dua istilah yang digunakan oleh kerajaan pusat terhadap daerah bawahannya. Pertanyaannya, mengapa ada daerah bawahan yang disebut wanua dan yang lainnya disebut palili. Apakah kriteria yang mesti dipunyai satu daerah untuk disebut sebagai wanua dan apakah pula kriteria yang mesti dipunyai satu daerah untuk dis...

  19. Analisis Kesesuaian dan Daya Dukung untuk Wisata Pantai dan Snorkeling di Pulau Hoga, Kabupaten Wakatobi.

    OpenAIRE

    Ahmad, Bahar

    2014-01-01

    Kawasan wisata bahari agar dapat dimanfaatkan secara berkelanjutan maka pemanfaatan yang dilakukan harus berkesesuaian dengan kondisi dan daya dukung kawasan. Penelitian ini bertujuan untuk menganalisis kesesuaian dan daya dukung untuk wisata pantai dan snorkeling di Pulau Hoga Kabupaten Wakatobi. Data yang digunakan dalam penelitian ini menggunakan data primer dan sekunder. Pengumpulan data primer menggunakan metode survei lapangan, sedangkan data sekunder diperoleh dari Badan Pusat Statis...

  20. KORUPSI DAN PELANGGARAN HAK EKONOMI, SOSIAL DAN BUDAYA DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Robby Darwis Nasution

    2016-02-01

    Full Text Available Korupsi merupakan budaya yang sudah lumrah terjadi di Indonesia, dalam keseharian perpolitikan di Indonesia bahkan tidak akan lepas dari aktifitas korupsi dimana pada kenyataannya korupsi sendiri akan berdampak sistemik bukan hanya berdapmapa kepada kemakmuran Negara tetapi juga berdampak kepada kesejahteraan masyarakat Indonesia. Kesejahteraan Masyarakat sesungguhnya dapat dilihat dari tingkat pemenuhan hak EKOSOB (Ekonomi, Sosial dan Budaya, ketiga poin tersebut merupakan bagian kecil indikator kesejahteraan rakyat. Bersama-sama dengan Hak Sipil, hak ekosob telah diakui secara internasional sebagai bagian dari the international bill of human rights. Kerangka hukumnya menjadi semakin jelas setelah hak-hak tersebut dituangkan dalam perjanjian multilateral yang tertuang dalam Covenan on Economic, Social and Cultural Rights (selanjutnya disingkat CESCR, yang disahkan oleh Majelis Umum PBB pada tahun 1966 sebagai pelaksanaan dari prinsip-prinsip yang dimuat dalam DUHAM 1948. Kewenangan negara yang dalam hal ini adalah pemerintah untuk menjamin hak-hak ekonomi, sosial dan budaya di indonesia dirasa tidak memenuhi hasil yang memadai sehingga dapat dilihat dengan masih banyaknya pengangguran, kemiskinan, dan rendahnya tingkat pendidikan warga negara. Selain itu, tingkat korupsi di Indonesia masih sangat tinggi dibanding negara-negara lain yang menyebabkan tingkat pelanggaran hak EKOSOB di Indonesia masih sangat tinggi.

  1. KEBEBASAN BERAGAMA DAN NORMA-NORMANYA

    Directory of Open Access Journals (Sweden)

    Abdillah Halim

    2013-04-01

      Abstrak Perdebatan tidak berkesudahan terus mewarnai perbincangan dan praktek kebebasan beragama, terutama mengenai kata “kebebasan” yang dilekatkan pada agama dan kepercayaan. Jika “kebebasan” dimaknai sebagai kemerdekaan, apa lantas berarti tidak adanya batasan terhadap kemerdekaan tersebut, dalam arti bahwa kebebasan tersebut bersifat mutlak. Atau barangkali “kebebasan” di sini dimaknai sebagai kebebasan relatif yang membuka kemungkinan perumusan definisi dan ruang lingkup yang jelas. Penulis berpandangan bahwa kebebasan beragama bukan kebebasan mutlak yang tidak menuntut pendefinisian, perumusan ruang-lingkup serta norma norma, dan pengaturan. Kebebasan mutlak pada taraf yang demikian bukan kebebasan yang bermakna sebenarnya, namun dapat berarti sebuah keadaan anarki dan anomi. Kebebasan beragama adalah kebebasan relatif yang menuntut adanya penjelasan tentang definisi, ruang lingkup, norma-norma, dan batasan-batasannya.

  2. Persebaran Layanan dan Infrastruktur Telekomunikasi Provinsi Papua

    Directory of Open Access Journals (Sweden)

    Prajna Deshanta Ibnugraha

    2014-05-01

    Full Text Available Layanan dan infrastruktur telekomunikasi merupakan bagian penting dari kehidupan modern karena hampir di segala bidang membutuhkan hal tersebut seperti pendidikan, pemerintahan, ekonomi dan sebagainya. Oleh karena pentingnya sektor telekomunikasi di Indonesia sebagai negara kepulauan maka perlu evaluasi tentang penyebaran layanan dan infrastrukturnya selain dilihat dari sisi fungsional dan perkembangan teknologinya. Provinsi Papua adalah provinsi terbesar di Indonesia namun justru memiliki layanan telekomunikasi yang paling sedikit dan terbatas. Demikian pula untuk penyebaran layanan ke kabupaten maupun ke distrik (kecamatan masih belum merata. Hal tersebut terbukti dari laporan hasil distribusi pita frekuensi yang hanya 1,5% untuk kawasan Maluku dan Papua serta kerapatan layanan tiap kabupaten yang ditunjukkan pada hasil penelitian ini.

  3. ANALISIS KUALITAS LINGKUNGAN PERAIRAN BERDASARKAN KOMUNITAS MEIOBENTOS DAN KUALITAS SEDIMEN DI PANTAI DAN AREA PERTAMBAKAN, PESISIR SRIWULAN KABUPATEN DEMAK (The Quality Analysis of Aquatic Environment based on Meiobentos Community and Sediment Quality

    Directory of Open Access Journals (Sweden)

    Muhandis Sidqi

    2015-01-01

    Full Text Available ABSTRAK  Tujuan studi ini meliputi (1 menentukan kualitas lingkungan daerah pesisir dan tambak ikan, kualitas sedimen, dan komunitas meiobentos, (2 menemukan hubungan antara kualitas sedimen dan meiobentos, dan (3 menentukan tingkat produktivitas peikampungan tambak ikan berdasarkan jarak, tingkat polusi dan destruksi. Parameter kualitas air dianalisis menggunakan metode deskriptif-komparatif, sedangkan parameter sedimen dianalisis menggunakan PCA (Principal Component Analisys untuk menentukan distribusi spasial pada setiap stasiun pemantauan dan lapisan kedalaman sedimen. Komunitas meiobentos diperiksa dengan menggunakan CA (Factorial Correspondence Analysis untuk mendeteksi tingkat distribusi spasial yang juga berdasarkan stasiun pemantauan dan lapisan kedalaman sedimen. Data tersebut dianalisis menggunakan korelasi dan regresi untuk memahami pengaruh parameter bebas terhadap produktivitas tambak ikan. Kemudian tes statistik non parametric dari Kruskall Wallis digunakan untuk membedakan produktivitas pada 3 desa berdasarkan jarak terhadap sumber pencemaran dan tingkat destruksi tambak ikan. Penelitian ini menemukan bahwa nilai parameter kualitas air (muddy, TSS NH3, NO2, beyond threshold level, negative redox potential (Eh sediment value/reduction zone, and IMLP adalah moderat. Hal ini menunjukkan bahwa kondisi air di daerah penelitian tidak stabil. Penelitian juga menunjukkan nilai indeks diversitas yang rendah, dominasi organisme meiobentos tertentu, dan konformitas antar komunitas bentos. Hasil tes statistik Kruskall Wallis menunjukkan adanya signifikansi antara produktivitas tambak ikan dengan factor jarak dan tingkat destruksi diantara 3 desa dalam daerah penelitian yaitu Bedono, Sriwulan. Purwosari yaitu dengan nilai produktivitas 0.65,0.56, 0.41 ton/hektar/tahun.   ABSTRACT The objective of this study area to determine the environmental quality of coastal area and fish ponds which are on water quality, sediment quality, and Meiobentos

  4. Three Kinds of Fish

    DEFF Research Database (Denmark)

    Høst, Jeppe Engset

    2012-01-01

    There are three kinds of fish. Fish you were given, fish you bought and fish you lease. This might sound a bit odd, but it is nevertheless the basis for the activities of Danish commercial fishers since the introduction of transferable fishing concessions (TFCs) in 2007. In the current 2012 reform...... of market based systems are wild speculation, concentration and monopolization of fishing access and subsequent leasing with fishing communities and new entrants very likely being worse off (see for example the chapter “From fishing rights to financial derivatives” is this volume or Olson 2011; Sumaila 2010...... will examine five Danish fishing operations and discuss how they have reacted in different ways to the newly introduced system of transferable fishing concessions. By introducing TFCs as a solution to fleet overcapacity, the EU Commission will also be introducing a system where buying, selling and leasing...

  5. DISTRIBUSI UKURAN, REPRODUKSI DAN HABITAT PEMIJAHAN IKAN BILIH (Mystacoleucus padangensis Blkr. DI DANAU SINGKARAK

    Directory of Open Access Journals (Sweden)

    Hafrijal Syandri

    2016-03-01

    Full Text Available Penelitian tentang biologi reproduksi ikan bilih di Danau Singkarak dilakukan pada bulan Januari –Desember 2010. Tujuan penelitian ini adalah untuk mengetahui distribusi ukuran, tingkat kematangan gonad, fekunditas dan pemijahan ikan bilih. Hasil penelitian menunjukkan ukuran panjang ikan bilih betina matang gonad berkisar antara 70-109 mm dan bobot tubuh berkisar antara 6,4-8,7 gram, ikan jantan pada panjang antara 70-89 mm dengan bobot antara 4,5-6,6 gram. Persentase ikan betina yang memijah setiap stasiun berkisar 68,4-75,7% dan ikan jantan berkisar 73,4-78,4%. Pada saat memijah ikan bilih beruaya dari danau ke sungai Sumpur, Paninggahan dan Baing setiap hari dimulai pukul 16.00 hingga 23.00 WIB. Karakteristik habitat pemijahan mempunyai kecepatan arus sungai antara 10-15 m/detik, kedalaman perairan berkisar antara 20-40 cm, substrat dasar perairan terdiri dari kerikil dan karakal. Study of biology reproduction bilih fish on Lake Singkarak has done a series of studies in January and December 2010. The purpose of this study is to reveal the size distribution, gonada mature level, fecundity and spawning of bilih fish. The research proves that the size of mature female fish gonads bilih range in size of 70-109 mm with a weight of 6.4 to 8.7 g and males 70-89 mm and weighs 4.4 to 6.6 g. Percentage of female fish to spawn each research station ranged from 68.5-75.7 % and males 73.4-78.3%. Bilih spawning fish populations by conducting migration from lakes to rivers Sumpur, Paninggahan and Baing everyday starting at 16:00 until 23:00 am. Characteristics of spawning habitat with river flow velocity between 10-15 m / sec, water depth between 20-40 cm, bottom substrate consists of gravel and karakal.

  6. MANAJEMEN BIMBINGAN DAN KONSELING BERBASIS PERMENDIKBUD NOMOR 111 TAHUN 2014

    Directory of Open Access Journals (Sweden)

    Edris Zamroni

    2015-11-01

    Full Text Available Manajemen bimbingan dan konseling adalah segala upaya atau cara yang digunakan  untuk mendayagunakan secara optimal semua komponen atau sumber daya (tenaga, dana, sarana-prasarana dan sistem informasi berupa himpunan data bimbingan dan konseling untuk menyelenggarakan pelayanan bimbingan dan konseling dalam rangka mencapai tujuan yang telah ditentukan. Permendikbud Nomor 111 Tahun 2014 diterbitkan untuk menjadi acuan baru pelaksanaan tata kelola bimbingan dan konseling mulai dari planning, organizing, staffing, leading dan controlling.

  7. Imunopatogenesis Treponema pallidum dan Pemeriksaan Serologi

    OpenAIRE

    Efrida ,; Elvinawaty .

    2014-01-01

    AbstrakSifilis adalah penyakit menular seksual yang sangat infeksius, disebabkan oleh bakteri berbentuk spiral, Treponema pallidum subspesies pallidum. Penyebaran sifilis di dunia telah menjadi masalah kesehatan yang besar dengan jumlah kasus 12 juta pertahun. Infeksi sifilis dibagi menjadi sifilis stadium dini dan lanjut. Sifilis stadium dini terbagi menjadi sifilis primer, sekunder, dan laten dini. Sifilis stadium lanjut termasuk sifilis tersier (gumatous, sifilis kardiovaskular dan neurosi...

  8. Manifestasi Kehamilan Di Rongga Mulut Dan Perawatannya

    OpenAIRE

    Fatimah

    2008-01-01

    Kehamilan merupakan sebuah proses fisiologis yang melibatkan banyak sistem dalam tubuh dan mengakibatkan terjadinya perubahan pada sistem baik secara anatomis maupun fisiologis seperti pada sistem reproduksi, endokrin, sirkulasi pencernaan dan juga rongga mulut. Manifestasi kehamilan di rongga mulut merupakan suatu kondisi yang sering tetjadi, baik pada gigi maupun pada jaringan di sekitamya. Hal ini dapat terlihat dan tingginya tingkat karies, erosi enamel, terjadinya gingivitis kehamila...

  9. Perkembangan Pendidikan Meunasah dan Dayah di Aceh

    OpenAIRE

    Mujianto Solichin

    2015-01-01

    Meunasah dan Dayah dalam masa perjalanannya tumbuh seiring perkembangan kerajaan-kerajaan Islam di Aceh yang memiliki peran strategis dalam pembentukan kebudayaan Islam di wilayah kekuasaan mereka masing-masing. Ini membuktikan bahwa kerajaan-kerajaan Melayu baik di Malaka (Malaysia) maupun di Aceh (Nangroe Aceh Darussalam) memiliki jaringan arkeologi sejarah kebudayaan dan peradaban yang terbentuk berdasarkan motivasi agama dan menjadikan tradisi Melayu sebagai identitas pemersatu mereka. Ke...

  10. KEBEBASAN BERAGAMA DAN NORMA-NORMANYA

    OpenAIRE

    Abdillah Halim

    2013-01-01

    KEBEBASAN BERAGAMA DAN NORMA-NORMANYA Abdillah Halim Jurusan Syariah, Sekolah Tinggi Agama Islam (STAI) Ngawi   Abstrak Perdebatan tidak berkesudahan terus mewarnai perbincangan dan praktek kebebasan beragama, terutama mengenai kata “kebebasan” yang dilekatkan pada agama dan kepercayaan. Jika “kebebasan” dimaknai sebagai kemerdekaan, apa lantas berarti tidak adanya batasan terhadap kemerdekaan tersebut, dalam arti bahwa kebebasan tersebut bersifat mutlak. Atau barangkali “ke...

  11. Reviews in fluorescence 2010

    CERN Document Server

    Geddes, Chris D

    2011-01-01

    ""Reviews in Fluorescence 2010"", the seventh volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year's progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. ""Reviews in Fluorescence"" offers an essential reference material for any lab working in the fluoresc

  12. Principles of fluorescence techniques

    CERN Document Server

    2016-01-01

    Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

  13. Bahasa Roh: Apa dan Bagaimana?

    Directory of Open Access Journals (Sweden)

    Hermanto Suanglangi

    2005-04-01

    Full Text Available Apakah itu glossalalia? Glossalalia adalah adalah kata 1.ang dibangun daridtra kata Cerika, yaitu " glossa" yang berarti bahasa atau lidah dan "lalia"artinva pembicaraan, perkataan. Sehingga "glossalalia" adalah suatu pembicaraan dengan lidah, atau perkataan denganlidah. Dari istilah inilah maka orang biasa menyebutnya dengan bahasa roh dan bahasa lidah, di mana bahasa roh, atau bahasa lidah adalah semacam bahasa yang dengan kemampuan Roh Kudus orang dapat mengucapkannya. Baik dalam Korintus maupun dalam Kisah Para Rasul, sama-sama disebutbahasa roh karena keduanya semacam bahasa yang diucapkan dengan kemampuan dari Roh yang sama yaitu Roh Kudus.

  14. KOMPLIKASI, PENCEGAHAN DAN PENANGANAN EKSTRAVASASI AGEN KEMOTERAPI

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    I G A Mirah K

    2012-12-01

    Full Text Available Ekstravasasi agen kemoterapi ke jaringan sekitarnya merupakan kecelakaan yangdapat menyebabkan kerusakan jaringan progresif ireversibel dalam hitungan jamsampai hari. Manifestasi klinis ekstravasasi berupa nyeri, edema, eritema, danindurasi yang kemudian berkembang menjadi ulkus dan eschar hitam dankerusakan jaringan yang mendasarinya. Pencegahan terjadinya ekstravasasi dapatdilakukan dengan menggunakan pembuluh darah yang paten dan dengan aliranyang cepat dan tetap memperhatikan keluhan yang disampaikan pasien. Setiaptenaga kesehatan yang akan menangani pasien kanker dengan kemoterapi, dituntutmemiliki pengetahuan mengenai ekstravasasi agen kemoterapi yang bergunadalam meningkatkan pelayanan pada pasien dan mengurangi morbiditas.

  15. Sistem Pengukur Suhu dan Kelembaban Ruang Server

    Directory of Open Access Journals (Sweden)

    Muhammad Fahmi Awaj

    2014-01-01

    Full Text Available Makalah ini membahas tentang perancangan sistem pengukur suhu dan kelembaban ruang server yang dapat meningkatkan efisiensi daya listrik dengan menggunakan arduino. Sistem terdiri dari sensor DHT 11 yang berfungsi untuk mengambil data berupa suhu dan kelembaban ruang server kemudian ditampilkan dalam LCD 16x2. Data suhu dan kelembaban ditampilkan juga ke dalam website dengan menggunakan IP address tertentu yang sebelumnya board arduino telah terhubung dengan ethernet shield. Suhu yang melebihi 270C akan menyalakan relay dan kipas serta sistem akan mengirim peringatan sms secara otomatis kepada administrator ruang server melalui sistem sms secara periodik hanya setiap tiga detik. Sistem peringatan sms akan berhenti jika suhu kurang 270C.

  16. KECEMASAN DAN STRATEGI COPING WARIA PELACUR

    Directory of Open Access Journals (Sweden)

    Haris Herdiansyah

    2007-05-01

    Full Text Available Dunia pelacuran adalah penuh dengan tekanan, ancaman dan hal negatif cacat yang menyebabkan ketertarikan kepada pelacur oleh karena itu mendorong pelacur untuk menciptakan strategi menghadapi cukup untuk menghadapi apa yang rasa mereka . Tujuan dari studi ini akan meliputi ke luar ketertarikan dan strategi pelacur yang menghadapi transgender di tiga lingkungan sosial berbeda termasuk dalam keluarga, masyarakat dan “ cebongan” ( tempat di mana pelacur bekerja. Ini adalah suatu riset kualitatif. Karena alat pengumpulan data adalah wawancara, dan pengamatan. Studi ini menggunakan purposive sampling. Ada 3 subjek dan 4 orang informan. Untuk menganalisis data menggunakan model interaktif dari Miles & Huberman yang terdiri dari 3 langkah, yaitu reduksi data, menampilkan data dan kesimpulan serta verifikasi. Hasil dari penelitian ini, banyak kecemasan yang dialami oleh waria pelacur yang datang dari lingkungan yang berbeda-beda. Dari keluarga misalnya, merupakan sumber kecemasan tertinggi, ketika keluarga waria mengetahui profesi mereka. Sumber lain berasal dari pengucilan oleh masyarakat dan mucikari, penangkapan oleh polisi dan preman, persaingan antar pelacur dan kekerasan dari pengguna mereka di “cebongan”

  17. KONTEKTUALISASI DAN (KEMUNGKINAN KONSEKUENSINYA BAGI PSIKOLOGI

    Directory of Open Access Journals (Sweden)

    Prof. Supratiknya

    2015-09-01

    jaran di perguruan tinggi seperti kita kenal sekarang (selanjutnya disebut psikologi, lahir di Jerman pada penghujung abad ke‐19. Selanjutnya psikologi berkembang pesat di Amerika Utara khususnya Amerika Serikat dan negara‐negara lain di Eropa yang lebih dulu mengalami industrialisasi khususnya di Eropa Barat. 1 Alhasil, paling tidak sampai dasawarsa 1980‐an perkembangan dan persebaran psikologi di tingkat global ditandai oleh ketimpangan di antara apa yang oleh Moghaddam (1987 disebut tiga dunia tempat psikologi dikembangkan dan dipraktekkan, disimak antara lain berdasarkan besarnya produksi pengetahuan psikologis yang disebarluaskan melalui penyelenggaraan pendidikan tinggi psikologi maupun lewat publikasi ilmiah berupa baik berkala maupun buku rujukan dan buku teks.

  18. TASAWUF DAN TANTANGAN PERUBAHAN SOSIAL

    Directory of Open Access Journals (Sweden)

    Yusno Abdullah Otta

    2010-12-01

    Full Text Available Abstract: Sufism in the eras of globalization and information often faces considerable challenges. The function and role of religion in modern life have in some aspects diminished, including sufism because it is claimed to have played no or less role in resolving problems nor stimulating social change. As one of Islamic traditions, sufism makes ihsan as its essential teaching. By this concept, sufism encourages humans to pray to God wholeheartedly. Sufism is now required to show its own existence and function by making fundamental contribution for modern life, an era which differ socio-culturally from the one when sufism firstly emerged. Sufism thus needs to adapt itself to the changes in the era of information and globalization so that it will remain relevant and suitable for human progress. Sufism needs to release itself from its exclusivism and to avoid being trapped in its own logic, namely speculative-trancendentalism Abstrak: Tasawuf di era globalisasi dan informasi seperti saat ini tengah menghadapi tantangan yang sangat berat. Kehidupan modern sudah sampai pada taraf tidak lagi mempercayai fungsi dan peran agama, termasuk di dalamnya tasawuf, sebagai ‘sesuatu’ yang dapat memberikan solusi bagi perubahan sosial. Sebagai manifestasi paripurna dari ajaran Islam, tasawuf berintikan pada konsep Ihsān yang mengajarkan untuk beribadah kepada Sang Pencipta secara ikhlas. Untuk menunjukkan eksistensinya, tasawuf dituntut peran aktifnya secara konstruktif-solutif terhadap berbagai kebutuhan manusia modern yang amat berbeda dengan setting maupun struktur masyarakat pada saat tasawuf “dilahirkan” pada zamannya. Tasawuf dituntut untuk memerlukan “baju” baru di era informasi yang kerap berubah ini, agar tetap realible dan suitable dengan kemajuan zaman. Hal itu dapat dilakukan dengan berusaha “keluar” dari eksklusifismenya dengan meminjam tradisi filsafat analitik pasca positivisme dan tradisi eksistensialis serta dinamika filsafat

  19. POTENSI DAN TINGKAT PEMAFAATAN IKAN SEBAGAI DASAR PENGEMBANGAN SEKTOR PERIKANAN DI SELATAN JAWA TIMUR

    Directory of Open Access Journals (Sweden)

    Nurul Rosana

    2015-10-01

    Full Text Available Jawa Timur (Jatim adalah salah satu propinsi yang memiliki potensi sumberdaya perikanan laut yang terdiri dari ikan pelagis dan ikan demersal. Wilayah pengelolaan perikanan laut di Jawa Timur bagian selatan memiliki potensi yang sangat besar karena berhadapan langsung dengan samudera Hindia dan memiliki potensi ikan khususnya kelompok pelagis besar seperti tuna (Thunnus sp dan cakalang (Katsuwonus pelamis. Untuk mengetahui informasi tentang potensi dan tingkat pemanfaatan perikanan laut di Jatim perlu dianalisis data yang ada, sehingga dapat digunakan sebagai acuan dalam mengembangkan sektor perikanan laut kedepannya dengan memperhatikan kelestarian sumberdayanya. Tujuan penelitian adalah mengetahui potensi dan tingkat pemanfaatan ikan di wilayah Selatan Jatim. Metode penelitian yang digunakan adalah metode survey dengan analisa data menggunakan metode Maximum Sustainable Yield (MSY. MSY atau potensi lestari ikan adalah besarnya jumlah stok ikan tertinggi yang yang dapat ditangkap secara terus menerus dari suatu sumberdaya tanpa mempengaruhi kelestarian stok ikan tersebut. Dengan menggunakan metode surplus produksi, potensi lestari ikan (MSY di wilayah Selatan Jatim periode 2009-2013 adalah sebesar 219.189,453 ton. Tingkat pemanfaatan ikan di perairan Selatan Jatim tahun 2009-2013 nilai rata-ratanya sebesar 49,48% yang berarti masih dibawah nilai jumlah tangkapan yang diperbolahkan (JTB sebesar 80%. Dari nilai tingkat pemanfaatan selama 5 (lima tahun tersebut dapat dikatakan perairan Selatan Jatim masih dalam kondisi underfishing karena tingkat pemanfaatannya masih dibawah nilai JTB (kurang dari 80%. Rata-rata nilai upaya penangkapan sebesar 577.764 trip dengan upaya optimum sebesar 523.437 trip, maka dapat disimpulkan bahwa upaya optimum lebih rendah dibandingkan dengan nilai rata-rata upaya penangkapan selama 5 (lima tahun.Kata Kunci: CPUE, MSY, potensi, tingkat pemanfaatan, upaya UTILIZATION POTENTIAL AND LEVEL OF FISH AS A BASIS FOR

  20. Analisis Kinerja EIGRP dan OSPF pada Topologi Ring dan Mesh

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2016-02-01

    Full Text Available ABSTRAK EIGRP (Enhanced Interior Gateway Routing Protocol dan OSPF (Open Shortest Path Fisrt adalah routing protokol yang banyak digunakan pada suatu jaringan komputer. EIGRP hanya dapat digunakan pada perangkat Merk CISCO, sedangkan OSPF dapat digunakan pada semua merk jaringan. Pada penelitian ini dibandingkan delay dan rute dari kedua routing protokol yang diimplementasikan pada topologi Ring dan Mesh. Cisco Packet Tracer 5.3 digunakan untuk mensimulasikan kedua routing protokol ini. Skenario pertama adalah perancangan jaringan kemudian dilakukan pengujian waktu delay 100 kali dalam 5 kasus. Skenario kedua dilakukan pengujian trace route untuk mengetahui jalur yang dilewati paket data lalu memutus link utama. Pada skenario kedua juga dilakukan perbandingan nilai metric dan cost hasil simulasi dengan perhitungan rumus. Skenario ketiga dilakukan pengujian waktu konvergensi untuk setiap routing protokol pada setiap topologi. Hasilnya EIGRP lebih cepat 386 µs daripada OSPF untuk topologi Ring sedangkan OSPF lebih cepat 453 µs daripada EIGRP untuk topologi Mesh. Hasil trace route menunjukan rute yang dipilih oleh routing protokol yaitu nilai metric dan cost yang terkecil. Waktu konvergensi rata-rata topologi Ring pada EIGRP sebesar 12,75 detik dan 34,5 detik pada OSPF sedangkan topologi Mesh di EIGRP sebesar 13 detik dan 35,25 detik di OSPF. Kata Kunci : EIGRP, OSPF, Packet Tracer 5.3, Ring, Mesh, Konvergensi ABSTRACT EIGRP (Enhanced Interior Gateway Routing Protocol and OSPF (Open Shortest Path Fisrt is the routing protocol that is widely used in a computer network. EIGRP can only be used on devices Brand CISCO, while OSPF can be used on all brands of network. In this study comparison of both the delay and the routing protocol implemented on Ring and Mesh topology. Cisco Packet Tracer 5.3 is used to simulate both the routing protocol. The first scenario is the design of the network and then do the test of time delay 100 times in 5 cases. The

  1. Analisis Perbandingan Database Jurnal Elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink Bidang Manajemen dan Ekonomi

    OpenAIRE

    Karyatin, Arianiansyah

    2016-01-01

    Penelitian ini dilakukan pada situs database jurnal elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink. Tujuan penelitian ini adalah untuk mengetahui bagaimana konten (muatan) subjek kajian manajemen dan ekonomi pada database Emerald, ProQuest ABI/INFORM dan SpringerLink dan untuk mengetahui di dalam database manakah diantara database Emerald, ProQuest ABI/INFORM, dan Springerlink yang memuat bidang kajian manajemen dan ekonomi yang paling lengkap. Metode yang digunakan dalam pene...

  2. Analisis Perbandingan Database Jurnal Elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink Bidang Manajemen dan Ekonomi

    OpenAIRE

    Karyatin, Arianiansyah

    2016-01-01

    Penelitian ini dilakukan pada situs database jurnal elektronik Emerald, ProQuest ABI/INFORM dan SpringerLink. Tujuan penelitian ini adalah untuk mengetahui bagaimana konten (muatan) subjek kajian manajemen dan ekonomi pada database Emerald, ProQuest ABI/INFORM dan SpringerLink dan untuk mengetahui di dalam database manakah diantara database Emerald, ProQuest ABI/INFORM, dan Springerlink yang memuat bidang kajian manajemen dan ekonomi yang paling lengkap. Metode yang digunakan dalam pene...

  3. Fluorescence correlation spectroscopy

    NARCIS (Netherlands)

    Hink, M.A.; Verveer, P.J.

    2015-01-01

    Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecul

  4. Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique.

    OpenAIRE

    1993-01-01

    We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well...

  5. Safe biodegradable fluorescent particles

    Science.gov (United States)

    Martin, Sue I.; Fergenson, David P.; Srivastava, Abneesh; Bogan, Michael J.; Riot, Vincent J.; Frank, Matthias

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  6. KEJAHATAN PORNOGRAFI Upaya Pencegahan dan Penanggulangannya di Kabupaten Ponorogo

    Directory of Open Access Journals (Sweden)

    Irma Rumtianing Uswatul Hanifah

    2013-12-01

    Full Text Available Pemerintah telah mengeluarkan undang-undang No 44 Tahun 2008 tentang pornografi. Tujuan dari undang-undang tersebut salah satunya mewujudkan dan memelihara tatanan kehidupan masyarakat yang beretika, berkepribadian luhur, menjungjung tinggi nilai-nilai Ketuhanan Yang Maha Esa, serta menghormati harkat dan martabat kemanusiaan. Dalam perkembangannya, materi pornografi mengalami pertumbuhan dan penyebaran yang pesat seiring dengan perkembangan dan kemajuan teknologi informasi dan komunikasi. Peredaran dan penyebaran film porno kini semakin pesat karena ditopang dengan kecanggihan sarana informasi dan komunikasi salah satunya media internet yang bisa diakses oleh masyarakat pada setiap saat. Tulisan berikut akan mengkaji bagaimana upaya dan trategi yang dilakukan oleh Pemerintah Kabupaten Ponorogo dalam mencegah dan menanggulangi kejahatan pornografi di Kabupaten Ponorogo, apa hambatan dan bagaimana solusi dalam pencegahan serta penanggulangan kejahatan pornografi di Kabupaten Ponorogo serta bagaimana formulasi kebijakan untuk pencegahan dan penanggulangan kejahatan pornografi di Kabupaten Ponorogo. Kata kunci: Pornografi, Polres, KP3A

  7. Perbandingan Akurasi Pengukuran Suhu dan Kelembaban Antara Sensor DHT11 dan DHT22

    Directory of Open Access Journals (Sweden)

    Arief Hendra Saptadi

    2014-11-01

    Full Text Available Suhu dan kelembaban merupakan dua objek pengukuran yang acapkali terdapat di dalam sistem akuisisi data. Terdapat banyak piranti sensor yang berfungsi untuk mengukur dua objek tersebut dan akurasi merupakan salah satu parameter yang dapat digunakan untuk memilihnya. DHT11 dan DHT22 adalah sensor seri DHT dari Aosong Electronics yang dapat melakukan pengukuran suhu dan kelembaban secara serempak dengan keluaran digital. Informasi tentang akurasi terdapat di dalam lembar data keduanya. Kendati pun demikian informasi tersebut tidak menggambarkan kondisi sesungguhnya saat dioperasikan pada lokasi maupun platform tertentu. Tujuan dari penelitian ini adalah untuk membandingkan akurasi dari DHT11 dan DHT22 dalam pengukuran suhu dan kelembaban saat dioperasikan di dalam maupun di luar ruangan, menggunakan platform ATMEL AVR dan Arduino. Berdasarkan pengujian yang telah dilakukan, DHT22 memiliki akurasi yang lebih baik daripada DHT11 dengan galat relatif pengukuran suhu 4% dan kelembaban 18%. DHT11 sebaliknya memiliki rentang galat yang lebih lebar sebesar 1 – 7% dan 11 – 35%, masing-masing untuk pengukuran suhu dan kelembaban. Perbedaan lokasi dan platform tidak memberikan pengaruh pada hasil pengukuran. Galat pengukuran kelembaban yang masih di atas 10% menunjukkan perlunya kalibrasi ulang. Di masa mendatang, selain akurasi, kepresisian juga perlu diuji menggunakan penghitungan simpangan baku pada hasil-hasil pengukuran.

  8. KANDUNGAN ASAM AMINO, TAURIN, MINERAL MAKRO-MIKRO, DAN VITAMIN B12 UBUR-UBUR (Aurelia aurita SEGAR DAN KERING

    Directory of Open Access Journals (Sweden)

    - - Nurjanah

    2014-06-01

    Full Text Available Tujuan penelitian adalah menentukan komposisi gizi, asam amino, taurin, mineral makro dan mikro,dan vitamin B12 pada ubur-ubur (Aurelia aurita segar dan kering. Asam amino esensial pada ubur-uburyaitu arginina, leusina, valina, treonina, lisina, isoleusina, fenilalanina, metionina, dan histidina, sedangkanasam amino non esensial yaitu asam glutamat, glisina, asam aspartat, serina, alanina, dan tirosina. Asamamino esensial tertinggi segar dan kering adalah arginina sebesar 1,72% (bk dan 1,44% (bk dan terendahhistidina yaitu sebesar 0,19% (bk dan 0,13% (bk. Asam amino non esensial segar dan kering tertinggiadalah asam glutamat dan glisina yaitu sebesar 3,26% (bk dan 2,62% (bk dan terkecil tirosina sebesar0,38% (bk dan 0,41% (bk. Taurin segar sebesar 2,68% (bk dan kering sebesar 0,67% (bk. Mineral makrotertinggi segar dan kering adalah natrium yaitu 180.092,1 ppm (bk dan 111.209,4 ppm (bk, terkecil adalahkalsium yaitu 5.750,2 ppm (bk dan 11,1 ppm (bk. Mineral mikro tertinggi segar dan kering adalah iodium yaitu8.291,5 ppm (bk dan 1.800 ppm (bk dan yang terkecil adalah tembaga yaitu 1,1 ppm (bk dan 0,6 ppm (bk.Vitamin B12 segar adalah 396,6 μm/100 g (bk dan kering 63,5 μm/100 g (bk.Kata kunci: asam amino, mineral, taurin, ubur-ubur (Aurelia aurita, vitamin B12

  9. IDENTIFIKASI DAN TEKNIK PENGENDALIAN HAMA DAN PENYAKIT BIBIT KRANJI (Pongamia pinnata

    Directory of Open Access Journals (Sweden)

    Tati Suharti

    2015-12-01

    Full Text Available Salah satu faktor pembatas dalam keberhasilan tanaman untuk tumbuh optimal yaitu adanya serangan hama dan penyakit. Identifikasi hama dan penyakit penting dilakukan karena berkaitan dengan teknik pengendalian. Tujuan penelitian ini adalah untuk mengetahui jenis hama dan penyakit yang menyerang bibit kranji (Pongamia pinnata dan teknik pengendaliannya. Metode penelitian meliputi identifikasi jenis hama dan penyakit serta teknik pengendalian dengan menggunakan pestisida biologi dan kimia. Hasil penelitian menunjukan, hama yang menyerang bibit kranji antara lain belalang (Valanga nigricornis, ulat grayak (Spodoptera sp., ulat jengkal (Hyposidra talaca dan ulat penggulung (Syllepta sp. sedangkan patogen yang menyebabkan penyakir hawar daun yaitu Phytophthora sp. Teknik pengendalian hama bibit kranji dengan menggunakan larutan ekstrak biji mahoni atau insektisida Bacillus thuringiensis sedangkan teknik pengendalian penyakit menggunakan larutan ekstrak biji mahoni, larutan ekstrak daun cengkeh atau benomil

  10. Petroleum hydrocarbon concentration in selected species of fish and prawn form northwest coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Mehta, P.; Kadam, A.N.; Gajbhiye, S.N.; Desai, B.N.

    Concentration of petroleum hydrocarbons in 6 fish and prawn species sampled at 10 transects from Veraval to Ratnagiri were determined using alkali digestion and alumina column chromatography followed by fluorescence spectroscopy. Efficiency...

  11. STRUKTUR KOMUNITAS DAN RELUNG MAKANAN IKAN PASCA INTRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus DI WADUK MALAHAYU, KABUPATEN BREBES

    Directory of Open Access Journals (Sweden)

    Kunto Purnomo

    2016-06-01

    Full Text Available Studi tentang struktur komunitas ikan dan pembagian sumber daya pakan ikan pasca introduksi ikan patin siam (Pangasianodon hypophthalmus di Waduk Malahayu (620 ha dilakukan dengan tujuan untuk mengetahui komposisi jenis ikan, preferensi makanan, dan luas relung tiap jenis ikan. Penelitian dilaksanakan dengan menggunakan metode survei pada bulan Agustus sampai Nopember 2009 dan bulan Maret sampai Oktober 2010. Hasil penelitian menunjukkan bahwa struktur komunitas ikan terdiri atas 13 jenis ikan, yang didominansi oleh ikan nila (Oreochromis niloticus, udang (Macrobrachium sp., dan gabus (Channa striata. Jenis-jenis sumber daya pakan yang dimanfaatkan oleh ikan adalah fitoplankton (20%, detritus (19%, zooplankton (17%, insekta (11%, tumbuhan air (9%, ikan (9%, udang (9%, dan moluska (6%. Ikan patin siam, mujair (Oreochromis mossambicus, dan beunteur (Puntius binotatus bersifat generalis karena mampu memanfaatkan semua sumber daya pakan alami yang tersedia. Ikan sili (Macrognathus aculeatus dan keting (Mystus nigriceps lebih bersifat spesialis karena hanya memanfaatkan insekta sebagai makanan utamanya. Peluang kompetisi antara ikan patin siam (diintroduksikan tahun 2009 dan ikan nila relatif kecil sebab sumber daya pakan utamanya berbeda, yaitu ikan patin siam memanfaatkan moluska sebagai makanan utamanya sedangkan ikan nila sebagian memanfaatkan fitoplankton. Hasil tangkapan ikan di waduk ini berkisar antara 34,3-1.323,1 ton/tahun dengan rata-rata 157,3 ton/tahun.   Malahayu Reservoir located in Brebes Regency, was impounded in 1930, with a surface area of 620 hectares, a mean water depth of 8 m. Its main function are flood control and irrigation. Study on fish community structure and food resource partitioning of fishes in Malahayu Reservoir were conducted from August to November 2009 and March to October 2010. The aim of the study was to evaluate the existing condition of fish resources, with emphasis on species composition, food preferency, and

  12. ANALISIS TINGKAT KEPUASAN DAN HARAPAN PASEN TERHADAP PELAYANAN PUSKESMAS DAN RUMAH SAKIT DAERAH PROVINSI DIY

    Directory of Open Access Journals (Sweden)

    Sunarto Sunarto

    2010-10-01

    Full Text Available Kepuasan merupakan perasaan seseorang mengenai kesenangan atau kekecewaan sebagai hasil membandingkan antara kinerja dan harapan. Jumlah kunjungan Puskesmas tahun 2007 sebesar 3.094.027 pasien yang terdiri dari 3.076442 rawat jalan dan 17.585 rawat inap. Penelitian untuk mengentahui gambaran tingkat kepuasan dan untuk mengetahui perbedaan tingkat kepuasan dan harapan pasien yang mendapatkan pelayanan Puskemas dan rumah sakit menurut wilayah Kabupatet / Kota di Propinsi DIY Penelitian ini merupakan penelitian deskriptif analitik dengan menggunakan rancangan cros sectional. Instrumen penelitian yang digunakan adalah kuisioner dengan 35 item pernyataan yang diambil dari atribut-atribut SERVQUAL dalam 5 dimensi. Total sampel untuk lima Kabupaten Kota sebanyak 1000, masing-masing Kabupaten Kota 200. Penelitian ini dilakukan terhadap pasien yang menerima pelayanan kesehatan di Puskesmas dan Rumah Sakit Daerah di Kabupaten-Kota Daerah Istimewa Yogyakarta. Penilaian tingkat kepuasan pasien ketika mereka berobat ke Puskesmas dan Rumah Sakit di Propinsi DIY, dengan menggunakan skala O sampai 4. Hasilnya adalah dimensi tangibles, rata-rata kepuasan pasien (2,96 cukup dan harapan pasien (3,38 pada dimensi reliability, rata-rata kepuasan pasien (3,01 bagus dan harapan pasien (3,40. Pada dimensi responsiveness, rata-rata kepuasan pa-sien (3,07 bagus dan harapan pasien (3,42. Dimensi assurance, rata-rata kepuasan pasien (3, 1 0 bagus dan harapan pasien (3 ,42. pada dimensi emphaty, rata-rata kepuasan pasien (3,02 bagus dan harapan pasien (3,35. Hasil uji statistik didapatkan nilai p > 0.05, berarti pada alpha 5%, dapat disimpulkan bahwa tidak terdapat perbedaan tingkat kepuasan diantara kelima wilayah kabupaten/kota di DIY. Penilaian tingkat kepuasan pasien, ketika mereka berkunjung ke Puskesmas dan Rumah Sakit di DIY secara rata-rata bagus dan tidak ada perbedaan tingkat kepuasan menurut wilayah Kabupaten-Kota di Propinsi DIY. Kata Kunci : Kepuasan, harapan

  13. Determination of As,Hg and Cd in FAPAS Canned Fish in UK by Hydride Generation-Atomic Fluorescence Spectrometry%氢化物-原子荧光光谱法测定英国FAPAS鱼罐头中砷汞镉

    Institute of Scientific and Technical Information of China (English)

    叶海辉; 谢德芳; 谢轶; 吴学进

    2012-01-01

    [Objective] To prove that the determination of As, Hg and Cd by the hydride generation-atomic fluorescence spectrometry (HG-AFS) has reached the international level of advanced detection instrument. [ Method] The parameters and testing methods of the HG-AFS were optimized. Through the testing of samples and the recovery test, a set of methods were established for determining the three elements in FAPAS canned fish. [Result] By comparing the detection report provided by FAPAS with the results of multiple laboratories, the Z value was -0.2 for As, 0. 5 for Hg, and 0.4 for Cd, the evaluation of results was satisfactory. [ Conclusion] It is feasible'to apply the HG-AFS method for the determination of As, Hg and Cd in seafood products.%[目的]证实原子荧光仪对这砷汞镉3种元素的检测已达到国际先进检测仪器的水平.[方法]利用氢化物-原子荧光光谱法,优化仪器条件参数和试验方法,通过测试质控样品与加标回收试验,建立一套测试英国FAPAS鱼罐头中砷汞镉的方法.[结果]根据FAPAS提供回来的检测报告,与全球众多实验室比对的结果为:砷的Z值为-0.2,汞的Z值为0.5,镉的Z值为0.4,对结果的评价为很满意.[结论]应用氢化物-原子荧光光谱法对海产品中砷汞镉进行检测是可行的.

  14. PEMANFAATAN MINYAK BUAH MERAH, Pandanus conoideus Lam DAN CAROPHYLL PINK DALAM RANSUM PAKAN YUWANA IKAN KAKAP MERAH, Lutjanus sebae

    Directory of Open Access Journals (Sweden)

    Titiek Aslianti

    2016-11-01

    Full Text Available Ikan kakap merah, Lutjanus sebae termasuk komoditas budidaya yang banyak diminati konsumen. Kualitas benih untuk pembesaran selain ditentukan dari ukuran dan performansi fisik, juga berdasarkan warna ikan. Dalam penelitian ini telah dilakukan 4 perlakuan pakan yang diperkaya dengan Minyak Buah Merah (MBM dan Carophyll Pink (CP yaitu: (A 10 mL MBM/kg pakan; (B 10 mL MBM+1,4 g CP/kg pakan; (C 1,4 g CP/kg pakan, dan (D Kontrol. Hewan uji adalah yuwana kakap merah dengan rata-rata panjang dan bobot tubuh awal 11,32±0,76 cm dan 37,56±7,22 g. Ikan ditempatkan dalam bak fiber kapasitas 5 m3 dengan kepadatan 200 ekor/bak dan diberi pakan 2 kali sehari secara ad libitum serta dipelihara selama 2 bulan dengan pergantian air 200%-300%/hari. Kualitas yuwana diamati pada akhir penelitian melalui pengukuran panjang dan bobot tubuh, sedangkan performansi warna diamati dengan kamera digital dan dianalisis secara diskriptif menggunakan Microsoft Adobe Photoshop 8. Hasil penelitian menunjukkan bahwa MBM dan CP dapat dimanfaatkan dalam ransum pakan guna meningkatkan pertumbuhan dan performansi warna yuwana kakap merah. Pakan yang mengandung 10 mL MBM+1,4 g CP menghasilkan sintasan 94%, kenaikan pertumbuhan panjang dan bobot mutlak 58,92% dan 281%, konversi pakan 0,82 serta performansi yuwana berwarna merah cerah dengan nilai persentase rata-rata sebesar 89,58%±0,07%. Red emperor snapper, Lutjanus sebae is one of high demanded cultured fishes. The quality of seeds does not only depend on size and performance of fish, but also depends on fish color. A feeding experiment was conducted during two-months period by adding red fruit oil (RFO and carophyll pink (CP to every kilogram of pellet fed as the treatment i.e. (A 10 mL RFO/kg feed; (B 10 mL RFO+1.4 g CP/kg feed; (C 1.4 g CP /kg feed and (D Control. Two hundred seeds with initial total length of 11.32±0.76 cm and 37.56±7.22 g of body weight were stocked in a fiber glass tank of 5 m3. Feeding frequency was

  15. STRATEGI PERSONAL BRANDING FOTOGRAFER HOTEL DAN RESORT

    OpenAIRE

    GUSTAFIAN, THOMAS HENRY ADRIAN

    2014-01-01

    Identitas brand menjadi masalah penting dalam lingkungan bisnis yang semakin kompleks dan penuh dengan persaingan. Salah satu cara yang dapat dilakukan seorang entrepreneur untuk menunjukkan identitas brand adalah melalui personal branding. Strategi personal branding sangat dipengaruhi oleh konsep diri dan strategi komunikasi yang dimiliki oleh seorang entrepreneur. Tulisan ini menggambarkan bagaimana strategi personal branding mempengaruhi keberhasilan seorang entrepreneur

  16. Dhenggung Asmarandana dan Dhegung Banten: Sebuah Komparasi

    Directory of Open Access Journals (Sweden)

    Teguh -

    2013-11-01

    Full Text Available The Comparation of Dhenggung Asmarandana and Dhegung Banten. Penelitian ini membahas perbandinganantara gending Dhenggung Asmaradana gaya Surakarta dengan gending Dhegung Banten gaya Yogyakarta.Berdasarkan penelitian dapat disimpulkan bahwa kedua gending memiliki persamaan dan perbedaan. Persamaankeduanya terletak pada laras, pathet, bentuk gendhing, struktur gendhing, serta balungan gending. Sedangkanperbedaannya hanya pada nama dan garap tabuhan bonang.

  17. Radiations cosmiques : danger dans l'Espace

    Science.gov (United States)

    Bonnet-Bidaud, J. M.; Dzitko, H.

    2000-06-01

    Au sol, l'atmosphere nous protege plus ou moins bien. Mais dans l'espace ou a bord des avions de ligne, l'homme est directement expose aux rayonnements cosmiques qui peuvent etre mortels. Un veritable frein a la presence humaine prolongee dans l'espace. Une menace que les agences spatiales prennent tres au serieux.

  18. Vast voer in zoogperiode belangrijker dan gedacht

    NARCIS (Netherlands)

    Peet-Schwering, van der C.M.C.; Binnendijk, G.P.; Troquet, L.M.P.

    2012-01-01

    Biggen die voor spenen vast voer opnemen (eters) groeien na spenen circa 50 gram per dag sneller dan biggen die tijdens de zoogperiode geen vast voer opnemen (niet-eters). Daarnaast groeien zwaardere biggen bij spenen circa 50 gram per dag sneller dan lichtere biggen bij spenen. Dit blijkt uit onder

  19. KARAKTERISTIK, KESESUAIAN, DAN PENGELOLAAN LAHAN UNTUK TAMBAK BUDIDAYA DI KABUPATEN LUWU UTARA PROVINSI SULAWESI SELATAN

    Directory of Open Access Journals (Sweden)

    Hasnawi Hasnawi

    2016-11-01

    Full Text Available Kabupaten Luwu Utara (Lutra memiliki lahan potensial untuk tambak dan produktivitas tambaknya untuk budidaya udang dan ikan relatif rendah. Oleh karena itu, dilakukan survai untuk mengetahui karakteristik lahan dalam upaya menentukan kesesuaian dan pengelolaan lahan untuk budidaya tambak demi peningkatan produktivitas tambak di Kabupaten Lutra. Faktor yang dipertimbangkan dalam mengetahui karakteristik lahan adalah: tanah, topografi, hidrologi, vegetasi, dan iklim. Analisis spasial dalam Sistem Informasi Geografis digunakan untuk penentuan kesesuaian lahan budidaya tambak. Pengelolaan lahan ditentukan berdasarkan karakteristik lahan yang disesuaikan dengan teknologi dan komoditas yang dapat diaplikasikan di tambak. Kawasan pesisir Kabupaten Lutra didominasi oleh tanah bermasalah yaitu tanah sulfat masam, tanah gambut, dan tanah sulfat masam yang berasosiasi dengan tanah gambut. Tanah sulfat masam adalah jenis tanah dominan di Kabupaten Lutra yang memiliki potensi kemasaman dan unsur-unsur toksik yang tinggi dan sebaliknya unsur hara makro yang rendah dengan tekstur tanah yang  tergolong pasir berlempung dan lempung berpasir. Topografi lahan umumnya relatif datar dan elevasi yang tergolong rendah yang didominasi oleh vegetasi Sonneratia sp., Rhizophora sp., dan Nypa fruticans. Curah hujan yang rendah pada bulan September sampai Februari berdampak pada kondisi kualitas air yang lebih baik terutama pada salinitas, pH, Ca, dan Mg yang lebih tinggi daripada musim hujan. Hasil analisis menunjukkan bahwa lahan tambak di Kabupaten Lutra yang tergolong sangat sesuai (kelas S1 seluas 1.821,9 ha; tergolong cukup sesuai (kelas S2 seluas 368,2 ha; dan tergolong sesuai marjinal (kelas S3 seluas 3.268,1 ha. Untuk perbaikan tanah bermasalah tersebut dapat dilakukan melalui remediasi maupun dengan pemupukan. Coastal area of North Luwu Regency has suitable areas for brackishwater ponds, however many of those areas are low in productivity for shrimp and fish

  20. PENGARUH KOMUNIKASI INTERN DAN KEPEMIMPINAN KEPALA SEKOLAH TERHADAP EFEKTIVITAS KERJA GURU DAN KARYAWAN SMP NEGERI 1 SURUH KABUPATEN SEMARANG

    Directory of Open Access Journals (Sweden)

    - Muhsin

    2006-12-01

    Full Text Available Permasalahan yang dikaji dalam penelitian ini adalah : 1 Adakah pengaruh komunikasi intern terhadap efektivitas kerja guru dan karyawan? 2 Adakah pengaruh kepemimpinan kepala sekolah terhadap efektivitas kerja guru dan karyawan? 3 Seberapa besar pengaruh komunikasi intern dan kepemimpinan kepala sekolah terhadap efektivitas kerja guru dan karyawan? Tujuan penelitian ini adalah : 1 untuk mengetahui ada dan besarnya pengaruh komunikasi intern terhadap efektivitas kerja guru dan karyawan. 2 Untuk mengetahui ada dan besarnya pengaruh Kepemimpinan kepala sekolah terhadap efektivitas kerja guru dan karyawan. 3.Untuk mengetahui ada dan besarnya pengaruh komunikasi intern dan kepemimpinan kepala sekolah terhadap efektivitas kerja guru dan karyawan. Populasi dalam penelitian ini adalah semua guru dan karyawan yang ada di SMP Negeri 1 Suruh kabupaten Semarang yang berjumlah 40 orang.. Metode pengumpulan data menggunakan angket dan dokumentasi. Analisis data menggunakan  program statistic SPSS. Hasil penelitian menunjukkan adanya pengaruh positif antara Komunikasi intern dan kepemimpinan kepala sekolah terhadap efektivitas kerja guru dan karyawan sebesar 68,7%. Uji pengaruh secara parsial menunjukkan bahwa seluruh variabel bebas  Komunikasi intern dan kepemimpinan kepala sekolah berpengaruh secara signifikan terhadap Efektivitas kerja  dengan besarnya pengaruh masing-masing adalah 32,61% Untuk Komunikasi intern dan 25,62% untuk kepemimpinan kepala sekolah. Kata Kunci : Komunikasi Intern. Kepemimpinan Kepala Sekolah, Efektivitas Kerja

  1. KUALITAS MUTU BAHAN MENTAH DAN PRODUK AKHIR PADA UNIT PENGALENGAN IKAN SARDINE DI PT. KARYA MANUNGGAL PRIMA SUKSES MUNCAR BANYUWANGI

    Directory of Open Access Journals (Sweden)

    Dyah Agustin Wulandari

    2009-04-01

    Full Text Available Mutu ikan kaleng tergantung pada kesegaran bahan mentah, cara pengalengan, peralatan dan kecakapan serta pengetahuan pelaksana-pelaksana teknis, sanitasi dan higiene pabrik dan lingkungan.  Kesegaran bahan mentah sangat penting dalam industri perikanan.  Kesegaran adalah tolak ukur untuk membedakan ikan jelek dan bagus kualitasnya.  Bila kualitas bahan mentah bagus, maka produk yang dihasilkan juga bagus.  Untuk mengendalikan mutu produk yang dihasilkan perusahaan diperlukan suatu sistem yang terkendali dan dapat mengendalikan seluruh aktifitas yang mempengaruhi mutu produk. Khusus untuk produk perikanan lahirnya konsep HACCP mendorong negara-negara maju menerapkan sistem pengawasan mutu ini kepada produsen sebagai jaminan mutu produknya. Dari hasi studi pustaka dan penelitian serta pengamatan langsung pada PT. Karya Manunggal Prima Sukses Muncar,  ternyata diketahui bahwa mutu bahan baku dan produk akhir berupa ikan kaleng sardine saus tomat yang dihasilkan sesuai dengan standart mutu SNI 01-3548-1994.  Sedangkan penerapan konsepsi HACCP belum terlaksana dengan baik sehingga diperlukan perbaikan, baik GMP dan SSOP pada unit pengolahan.Kata kunci : Mutu ikan kaleng, HACCP.  The quality of a certain tinned-fish depends on several things; those are the freshness of raw materials, tinning technique, devices, knowledge, and capability of the technicians, sanitation and hygienist of the factory. The freshness of raw materials is important in fishery industries.  Freshness is one of indicators in determining fish quality. If raw materials are in good quality so that the product will be. To maintain quality of a product in certain factory, system that are able to control all of the elements affecting product quality, is absolutely needed. In fishery product, availability of HACCP, encourage the advanced-countries to apply a monitoring system of product quality to the producer as a kind of quality guarantee. From the study of certain literature

  2. PENANGKAPAN BENIH BETUTU (Oxyeleotris marmorata (Bleeker DENGAN PANGILAR (Fish Trap MENGGUNAKAN UMPAN HIDUP

    Directory of Open Access Journals (Sweden)

    Iriansyah Iriansyah

    2016-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui alat yang cocok untuk menangkap bibit benih betutu dalam keadaan hidup dan sehat. Hasil penelitian menunjukkan bahwa hasil tangkapan pangilar dalam jumlah (ekor dan berat (gram terdapat perbedaan hasil tangkapan antara perlakuan A dan B sebesar 7 ekor dan 1050 gram.  Jumlah total hasil tangkapan Pangilar (Fish Trap baik yang menggunakan umpan hidup dan yang tidak menggunakan umpan hidup adalah 75 ekor  atau dengan berat 5350 gram (5,350 Kg  dalam 16 kali pengambilan data dengan menggunakan 30 buah alat tangkap This study aims to determine a suitable tool to capture betutu seed seedlings alive and healthy. The results showed that the amount of the catch pangilar (tail and weight (g there are differences in catches between treatment A and B by 7 tails and 1050 grams. The total amount of the catch Pangilar (Fish Trap whether using live bait and live bait are not using tail is 75 or weighing 5350 grams (5.350 kg to 16 times the retrieval of data by using 30 pieces of gear.

  3. Penerapan Sistem Remunerasi dan Kinerja Pelayanan

    Directory of Open Access Journals (Sweden)

    Tri Wisesa Soetisna

    2015-08-01

    Full Text Available Remunerasi dapat memengaruhi motivasi pegawai sekaligus meningkatkan kinerjanya. Demikian halnya di rumah sakit sebagai institusi pelayanan kesehatan yang padat modal, sumber daya manusia serta padat ilmu dan teknologi. Penelitian ini bertujuan untuk menganalisis persepsi pegawai terhadap implementasi sistem remunerasi dan kinerja unit pelayanan bedah jantung dewasa (UPBJD di rumah sakit. Penelitian ini menggunakan pendekatan mixed methods (kuantitatif dan kualitatif. Desain penelitian kuantitatif adalah potong lintang menggunakan instrumen kuesioner self-assessment. Sedangkan desain penelitian kualitatif adalah deskriptif, dilakukan melalui focus group discussion dan telaah dokumen pada data berupa buku jadwal, buku registrasi, catatan keperawatan, dan rekam medis. Pengambilan data dilakukan pada tahun 2013 di salah satu rumah sakit di Jakarta. Responden/informan adalah staf medis fungsional, perawat, dan petugas administrasi berjumlah 29 orang. Data dianalisis secara univariat (metode kuantitatif, dan content analysis (metode kualitatif. Hasil penelitian menunjukkan bahwa sebagian besar staf medis fungsional dan perawat tidak puas (71,2% dengan beberapa hal dalam penerapan sistem remunerasi, seperti pada sistem penggajian dan penentuan grading. Terlihat kinerja unit pelayanan bedah jantung dewasa mengalami kenaikan setiap tahun sebelum dan setelah penerapan sistem remunerasi. Diharapkan agar rumah sakit ini dapat memperbaiki sistem remunerasi yang sesuai ketentuan kebijakan dan menyusun formulasi insentif dan bonus yang lebih sesuai dengan kondisi saat ini serta perlu dilakukan sosialisasi yang tepat dan evaluasi secara berkala. Implementation of Remuneration System and Service Performance Remuneration can influence worker`s motivation, and improve their performance. Likewise in hospital as capital-intensive, human resources-intensive as well as knowledge and technology-intensive health care institution. This study aimed to analyze employee

  4. RECOVERY OF THIRD-STAGE LARVAE OF ANGUILLICOLA CRASSUS (NEMATODA : DRACUNCULOIDEA) FROM THE FLESH OF FISHES : ASSESSMENT OF A DIGESTIVE MEDIUM AS A TOOL FOR THE STUDY OF FISH PARATENIC HOSTS. EXTRACTION DE LARVES DE TROISIÈME STADE D'ANGUILLICOLA CRASSUS (NEMATODA : DRACUNCULOIDEA) DANS LA CHAIR DE POISSONS : MISE AU POINT D'UNE MÉTHODE PAR DIGESTION POUR L'ÉTUDE DU PORTAGE PARASITAIRE PAR LES POISSONS HÔTES PARATÉNIQUES.

    OpenAIRE

    AGOULON A.; Blanc, G.; LE BRIS H.; Marchand, A

    2008-01-01

    The purpose of this study was to recover third-stage larvae (L3) of Anguillicola crassus alive from the flesh of fishes. Different digestive media have been tested on eel flesh for their efficiency and on living infective third-stage larvae for their harmlessness. The results were : (1) Long-term survival of the L3 larvae was low, but their morphological features were recognizable in each digestion runs. (2) Pepsin concentrations between 2.5 and 40 g/l proved to be without influence on the s...

  5. Umatilla - Rough Fish Eradication

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In order to enhance environmental conditions in the McCormack Slough on Umatilla NWR, the population of rough fish, including common carp (Cyprinus carpio) and...

  6. Textbook of fish health

    National Research Council Canada - National Science Library

    Post, George

    1987-01-01

    Written to fill the great need for a fish disease textbook for college students, this new edition contains the latest information and important discoveries related to those fish diseases that affect man economically...

  7. Scorpion fish sting

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/article/002849.htm Scorpion fish sting To use the sharing features on this page, please enable JavaScript. Scorpion fish are members of the family Scorpaenidae, which ...

  8. Pittsburgh Fish Fry Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Lenten Fish Fry records for the Greater Pittsburgh region. Data is collected before and during the Lenten fish fry season each year by Code for Pittsburgh. Data is...

  9. KARAKTERISASI SIMPLISIA DAN EKSTRAK DAUN STROBILANTHUS CRISPUS

    Directory of Open Access Journals (Sweden)

    Ani Isnawati

    2012-10-01

    Full Text Available Khasiat obat tradisional disebabkan oleh adanya senyawa kimia yang  dikandungnya. Bahan baku obat dari hasil pertanian atau kumpulan tumbuhan liar tentunya kandungan kimianya tidak dapat dijamin selalu konstan (ajeg karena adanya variabel bibit, tempat tumbuh, iklim, kondisi (umur dan cara panen. Kandungan senyawa kimia yang bertanggung jawab terhadap respon biologis harus mempunyai spesifikasi kimia, yaitu informasi  komposisi (jenis dan kadar. 0leh karena itu penetapan karakterisasi suatu simplisia dan ekstrak perlu dilakukan guna menjamin bahwa bahan suatu produk obat tradisional dapat diketahui mutunya. Karakterisasi dilakukan terhadap Tanaman Strobilanthus crispus (BL, yaitu simplisia bagian daun dan ekstrak 50% tanaman tersebut. Karakterisasi simplisa meliputi : penetapan kadar abu, kadar abu larut air, kadar abu tidak larut asam, kadar sari larut air, kadar sari larut asam, dan kadar air secar·a destilasi. Cara penetapan diatas dilakukan sesuai prosedur yang Ielah ditetapkan MMI, 1979. Sedangkan karakterisasi ekstrak mencakup : karakterisasi non spesifik yang meliputi penetapan bobot  jenis, kadar air, kadar sisa pelarut, kadar abu dan karakterisasi spesifik yang mencakup  pemeriksaan·senyawa yang terlarut dalam pelarut air dan etanol, pola kromatografi dengan cara KLT-densitometri, pemeriksaan golongan kimia ekstrak dan penetapan kadar zat kimia. Hasil penetapan karakterisasi simplisia menunjukkan spesifikasi tidak sesuai dengan yang dipersyaratkan MMI, hanya penetapan sari larut etanol yang memenuhi persyaratan. Penetapan karakterisasi ekstrak etanol 50% menunjukkan tidak terdapat etanol dalam ekstrak, kadar air 13,3 %; bobot jenis 1,262%, senyawa terlarut dalam pelarut air 95,06%, senyawa terlarut dalam pelarut etanol 18,69 % dan kadar flavonoid  17,59 % serta  profil  kromatogram komponen utama fraksi heksan, Khloroform dan etanol.

  10. Recent developments in fluorescence-based microscopy applied in biomedical sciences

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The present short review aims to give an overview of the most recent de velopments in fluorescence microscopy and its applications in biomedical science s. Apart from improvements in well-established methods based on conventional fl u orescence microscopy and confocal microscopy (fluorescence in situ hybridisa tion (FISH), tyramide signal amplification (TSA) in immunocytochemistry, new fluorop hores), more recently introduced techniques like fluorescence resonance energy t ransfer (FRET), fluorescence recovery after photobleaching (FRAP), multiphoton m icroscopy and fluorescence correlation spectroscopy (FCS) will be discussed.

  11. Plasmon assisted fluorescence emission: far-field observables and their fluctuations

    NARCIS (Netherlands)

    Langguth, L.A.

    2015-01-01

    Fluorescence describes the property of matter to absorb light and shortly after emit light of another color. Fluorescent materials are found in nature, e.g. in the jelly-fish Aequorea victoria, in some plants and mushrooms, and in minerals such as Fluorite in which this property was first

  12. Plasmon assisted fluorescence emission: far-field observables and their fluctuations

    NARCIS (Netherlands)

    L.A. Langguth

    2015-01-01

    Fluorescence describes the property of matter to absorb light and shortly after emit light of another color. Fluorescent materials are found in nature, e.g. in the jelly-fish Aequorea victoria, in some plants and mushrooms, and in minerals such as Fluorite in which this property was first scientific

  13. 49 CFR 173.218 - Fish meal or fish scrap.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Fish meal or fish scrap. 173.218 Section 173.218... Fish meal or fish scrap. (a) Except as provided in Column (7) of the HMT in § 172.101 of this subchapter, fish meal or fish scrap, containing at least 6%, but not more than 12% water, is authorized...

  14. FISH analysis in Prader-Willi and Angelman syndrome patients

    Energy Technology Data Exchange (ETDEWEB)

    Bettio, D.; Rizzi, N.; Giardino, D. [Centro Auxologico Italiano, Milan (Italy)] [and others

    1995-03-27

    We report on a combined high resolution cytogenetic and fluorescent in situ hybridization study (FISH) on 15 Prader-Willi syndrome (PWS) and 14 Angelman syndrome (AS) patients. High resolution banding showed a microdeletion in the 15q11-q13 region in 7 out of 15 PWS patients, and FISH analysis of the D15S11 and SNRPN cosmids demonstrated absence of the critical region in three additional cases. Likewise 8 out of 14 AS patients were found to be deleted with FISH, using the GABRB3 specific cosmid, whereas only 4 of them had a cytogenetically detectable deletion. 19 refs., 3 figs., 1 tab.

  15. Evaluation of the damage in fish spermatozoa cryopreservation

    Institute of Scientific and Technical Information of China (English)

    LI Jun; LIU Qinghua; ZHANG Shicui

    2006-01-01

    Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology,biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed.New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis,flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.

  16. Fluorescence of muscle and connective tissue from cod and salmon

    DEFF Research Database (Denmark)

    Andersen, Charlotte Møller; Wold, J.P.

    2003-01-01

    Autofluorescence of salmon and cod muscle was measured and compared with autofluorescence of collagen type I and type V. Similarities between fluorescence of fish muscle and collagen were found in that the same peaks were obtained around 390, 430, and 480 nm, These similarities are supported by p...

  17. Supernumerary ring chromosome 20 characterized by fluorescence in situ hybridization

    NARCIS (Netherlands)

    Van Langen, Irene M.; Otter, Mariëlle A.; Aronson, Daniël C.; Overweg-Plandsoen, W.C.G.; Hennekam, Raoul C.M.; Leschot, Nico J.; Hoovers, Jan M.N.

    1996-01-01

    We report on a boy with mild dysmorphic features and developmental delay, in whom karyotyping showed an additional minute ring chromosome in 60% of metaphases. Fluorescence in situ hybridization (FISH) with a centromere specific probe demonstrated that the ring chromosome contained the centromeric r

  18. Supernumerary ring chromosome 17 identified by fluorescent in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Fagan, K. [Hunter Area Pathology Service, New South Wales (Australia); Edwards, M. [Western Suburbs Hospital, New South Wales (Australia)

    1997-04-14

    We present a patient with multiple anomalies and severe developmental delay. A small supernumerary ring chromosome was found in 40% of her lymphocyte cells at birth. The origin of the marker chromosome could not be determined by GTG banding, but fluorescent in situ hybridization (FISH) later identified the marker as deriving from chromosome 17. 20 refs., 2 figs., 1 tab.

  19. Medusivorous fishes, a review

    NARCIS (Netherlands)

    Ates, R.M.L.

    1988-01-01

    A preliminary review is presented of fish species having consumed pelagic Cnidaria (Scyphozoa and Hydrozoa) as well as Ctenophora. Quantitative data are scarce. Knowledge of morphological and physiological adaptations of fishes foraging on gelatinous plankton is almost non-existent. Many fish specie

  20. Medusivorous fishes, a review

    OpenAIRE

    Ates, R.M.L.

    1988-01-01

    A preliminary review is presented of fish species having consumed pelagic Cnidaria (Scyphozoa and Hydrozoa) as well as Ctenophora. Quantitative data are scarce. Knowledge of morphological and physiological adaptations of fishes foraging on gelatinous plankton is almost non-existent. Many fish species consume medusae and some reasons to suspect that there are even more that do so, are discussed.

  1. Medusivorous fishes, a review

    NARCIS (Netherlands)

    Ates, R.M.L.

    1988-01-01

    A preliminary review is presented of fish species having consumed pelagic Cnidaria (Scyphozoa and Hydrozoa) as well as Ctenophora. Quantitative data are scarce. Knowledge of morphological and physiological adaptations of fishes foraging on gelatinous plankton is almost non-existent. Many fish

  2. The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon.

    Directory of Open Access Journals (Sweden)

    John S Sparks

    Full Text Available The discovery of fluorescent proteins has revolutionized experimental biology. Whereas the majority of fluorescent proteins have been identified from cnidarians, recently several fluorescent proteins have been isolated across the animal tree of life. Here we show that biofluorescence is not only phylogenetically widespread, but is also phenotypically variable across both cartilaginous and bony fishes, highlighting its evolutionary history and the possibility for discovery of numerous novel fluorescent proteins. Fish biofluorescence is especially common and morphologically variable in cryptically patterned coral-reef lineages. We identified 16 orders, 50 families, 105 genera, and more than 180 species of biofluorescent fishes. We have also reconstructed our current understanding of the phylogenetic distribution of biofluorescence for ray-finned fishes. The presence of yellow long-pass intraocular filters in many biofluorescent fish lineages and the substantive color vision capabilities of coral-reef fishes suggest that they are capable of detecting fluoresced light. We present species-specific emission patterns among closely related species, indicating that biofluorescence potentially functions in intraspecific communication and evidence that fluorescence can be used for camouflage. This research provides insight into the distribution, evolution, and phenotypic variability of biofluorescence in marine lineages and examines the role this variation may play.

  3. The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon.

    Science.gov (United States)

    Sparks, John S; Schelly, Robert C; Smith, W Leo; Davis, Matthew P; Tchernov, Dan; Pieribone, Vincent A; Gruber, David F

    2014-01-01

    The discovery of fluorescent proteins has revolutionized experimental biology. Whereas the majority of fluorescent proteins have been identified from cnidarians, recently several fluorescent proteins have been isolated across the animal tree of life. Here we show that biofluorescence is not only phylogenetically widespread, but is also phenotypically variable across both cartilaginous and bony fishes, highlighting its evolutionary history and the possibility for discovery of numerous novel fluorescent proteins. Fish biofluorescence is especially common and morphologically variable in cryptically patterned coral-reef lineages. We identified 16 orders, 50 families, 105 genera, and more than 180 species of biofluorescent fishes. We have also reconstructed our current understanding of the phylogenetic distribution of biofluorescence for ray-finned fishes. The presence of yellow long-pass intraocular filters in many biofluorescent fish lineages and the substantive color vision capabilities of coral-reef fishes suggest that they are capable of detecting fluoresced light. We present species-specific emission patterns among closely related species, indicating that biofluorescence potentially functions in intraspecific communication and evidence that fluorescence can be used for camouflage. This research provides insight into the distribution, evolution, and phenotypic variability of biofluorescence in marine lineages and examines the role this variation may play.

  4. TUGAS DAN WEWENANG PUSAT PELAPORAN DAN ANALISIS TRANSAKSI KEUANGAN (PPATK DALAM PEMBERANTASAN TINDAK PIDANA PENCUCIAN UANG

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    Johari Johari

    2014-03-01

    Full Text Available Tugas dan wewenang Pusat Pelaporan dan Analisis Transaksi Keuangan (PPATK terdapat di dalam Pasal 26 dan Pasal 27 Undang-Undang No. 25 Tahun 2003 tentang Tindak Pencucian Uang. Berdasarkan ketentuan tersebut, tugas dan wewenang PPATK tersebut bertujuan untuk mendeteksi terjadinya tindak pidana pencucian uang, dan membantu penegakan hukum yang berkaitan dengan pencucian uang, termasuk tindak pidana asal yang melahirkannya (predicate offences. Namun, Peranan PPATK akan berjalan secara efektif apabila aparat penegak hukum seperti Kepolisian, Kejaksaan, Pengadilan, Bea dan Cukai, para regulator seperti Bank Indonesia, Departemen Keuangan, Badan Pengawas Pasar Modal serta Penyedia Jasa Keuangan, industri perbankan, asuransi, perusahaan pembiayaan, dana pensiun, perusahaan efek, pengelola reksadana, media massa, masyarakat bekerjasama secara terorganisir dan terpadu dalam pemberantasan tindak pencucian uang di Indonesia. Dengan kewenangan yang dimilikinya, PPATK dapat mengejar hasil dari kejahatan, apabila hasil kejahatan tersebut dapat dikejar dan disita maka negara dengan sendirinya akan mengurangi tindak kejahatan itu sendiri. Kata kunci : Pencucian uang, tindak pidana pencucian uang (money laundering, kejahatan terorganisir, dan Pusat Pelaporan dan Analisis Transaksi Keuangan (PPATK.

  5. Peran Mitra Strategis dan Agen Perubahan dalam Manajemen Talenta dan Kinerja Manajer

    Directory of Open Access Journals (Sweden)

    Ida Ketut Kusumawijaya

    2014-06-01

    Full Text Available Abstract  The purposes of the study are to analyze: (1 characteristic of strategic partner and change agent roles, talent management and managers performance, (2 influence of strategic partner and change agent roles to talent management and its impact to managers performance. Four hundred hotel managers in Bali were participated in this study by using proportional random sampling. Data were analyzed using descriptive analysis and SEM. The result shows that: (1 strategic partner and change agent role, talent management and managers performance are in enough category, (2 positive and significant effect between strategic partner and change agent roles to talent management and its impact to managers performance. Keywords:Strategic Partner Role, Change Agent Role, Talent Management, Managers Performance.AbstrakTujuan penelitian ini adalah untuk menganalisis: (1 karakteristik dari mitra strategis dan peran agen perubahan, manajemen bakat dan kinerja manajer, (2 pengaruh mitra strategis dan peran agen perubahan untuk manajemen bakat dan dampaknya terhadap kinerja manajer. Sebanyak empat ratus manajer hotel di Bali berpartisipasi dalam penelitian ini dengan menggunakan proporsional random sampling. Data dianalisis menggunakan analisis deskriptif dan SEM. Hasil penelitian menunjukkan bahwa: (1 mitra strategis dan peran agen perubahan, manajemen bakat dan kinerja manajer termasuk dalam kategori cukup, (2 pengaruh positif dan signifikan antara mitra strategis dan peran agen perubahan untuk manajemen bakat dan dampaknya terhadap kinerja manajer.Keywords: Peran Mitra Strategis, Peran Agen Perubahan, Manajemen Talenta, kinerja Manajer.

  6. POLA KONSUMSI PANGAN DAN ASUPAN ENERGI DAN ZAT GIZI ANAK STUNTING DAN TIDAK STUNTING 0—23 BULAN

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    Aslis Wirda Hayati

    2013-10-01

    menganalisis pola konsumsi pangan dan asupan energi dan gizi anak stunting dan tidak stunting 0—23 bulan menggunakan data Riskesdas 2010. Sub-set data Riskesdas 2010 diperoleh dalam bentuk e-files. Dari 6 634 data anak baduta dalam e-files Riskesdas 2010, sejumlah 3 539 data anak dikeluarkankarena data tidak lengkap, pencilan, konsumsi pangan saat kondisi tidak biasa. Status gizi diolah menggunakan WHO AntroPlus 2007, pengolahan data lainnya menggunakan program Microsoft Excel 2007 dan SPSS for windows. Uji beda pola konsumsi pangan menerapkan Mann-Whitney U test. Pola konsumsi pangan dan asupan energi dan zat gizi yang diukur berupa jumlah jenis konsumsi pangan, jumlah kelompok konsumsi pangan, frekuensi konsumsi pangan, tingkat kecukupan zat gizi, mutu gizi asupan pangan, dan densitas asupan zat gizi. Hasil kajian menunjukkan pola konsumsi pangan dan asupan energi dan zat gizi anak stunting dan tidak stunting 0—23 bulan berbeda menurut kelompok umur; semakin bertambah umur semakin meningkat perbedaannya. Pada anak 0—5 bulan tidak ada perbedaan pola konsumsi pangan dan asupan energi dan gizi anak stunting dan tidak stunting; sebaliknya pada anak 6—11 dan 12—23 bulan. Pada anak 6—11 bulan, rata-rata tingkat kecukupan protein anak stunting dan tidak stunting berbeda, demikian pula densitas asupan protein.Pada anak 12—23 bulan, tidak hanya rata-rata tingkat kecukupan protein dan densitas asupan protein, tetapi rata-rata tingkat kecukupan energi, tingkat kecukupan kalsium dan densitas asupan kalsium, tingkat kecukupan fosfor, tingkat kecukupan vitamin A, tingkat kecukupan vitamin C, mutu gizi makanan, dan bahkan jenis pangan juga berbeda. Implikasi untuk Indonesia yaitu perlu dilakukan penelitian efikasi intervensi zat gizi tersebut untuk pencapaian pertumbuhan linier optimal anak.Kata kunci: anak usia 0—23 bulan, pola konsumsi pangan, stunting

  7. PEMANFAATAN SOSIAL MEDIA DALAM PENYULUHAN PERTANIAN DAN PERIKANAN DI INDONESIA

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    Kadhung Prayoga

    2017-04-01

    Full Text Available ABSTRAKPertukaran informasi menjadi masalah yang mendapat sorotan dalam kegiatan penyuluhan pertanian dan perikanan. Sulitnya petani mengakses informasi ini kemudian memunculkan solusi dengan memanfaatkan teknologi informasi seperti media sosial. Sehingga, paper ini bertujuan untuk mengetahui bagaimana pelaksanaan penyuluhan yang memanfaatkan media sosial ini lewat sebuah studi literature terhadap sumber data sekunder. Dari hasil analisis penggunaan facebook dirasa masih sangat kurang optimal karena tidak ada update informasi terkait kegiatan perikanan. Namun, Kementerian Pertanian justru sangat aktif dalam menggunakan facebook. Sedangkan untuk pemanfaatan twitter, keduanya sama-sama aktif untuk berinteraksi dengan masyarakat. Pemanfaatan video conference dinilai sangat baik untuk Pusat Penyuluhan dan Pemberdayaan Masyarakat Kelautan dan Perikanan, namun masih kurang dioptimalkan oleh Kementerian Pertanian. Secara rutin dua institusi ini memperbaharui informasi terkait kegiatan pertanian dan perikanan seperti: budi daya, teknologi, maupun pemasaran. Sehingga, dapat disimpulkan bahwa kegiatan penyuluhan yang memanfaatkan media sosial harus terus dioptimalkan karena jumlah penggunanya yang terus meningkat.UTILIZATION OF SOCIAL MEDIA IN AGRICULTURE AND FISHERIES EXTENSION ACTIVITY IN INDONESIAABSTRACTThe exchange of information becomes a problem that gets the spotlight in the agriculture and fisheries extension activities. The difficulty for farmers to access this information is then led to solutions that take advantage of information technology such as social media.Thus, this paper aims to determine how the implementation of social media outreach utilizing it through a literature study on secondary data sources. From the analysis of the use of facebook it is still a very sub-optimal because there is no update information related to fishing activities. However, the Ministry of Agriculture is very much active in using facebook. As for the use of

  8. KONSTRUKSI DETERMINAN DALAM FRASA NOMINA BAHASA PRANCIS DAN BAHASA INDONESIA

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    Roswita Lumban Tobing

    2012-06-01

    Full Text Available Artikel ini bertujuan untuk memaparkan konstruksi determinan pada frasa nomina bahasa Perancis dan bahasa Indonesia. Analisis yang digunakan adalah analisis kontrastif. Hasil analisis menunjukkan bahwa nomina bahasa Perancis memiliki jenis kelamin (maskulin/feminin dan jumlah (tunggal/jamak. Gender dan jumlah dalam bahasa Prancis memiliki aturan konkordansi yang dapat memengaruhi bentuk dan makna nomina. Dalam bahasa Indonesia, meskipun nomina memiliki konsep gender tunggal dan jamak, jenis dan jumlah dalam bahasa Indonesia tidak muncul secara eksplisit. Bentuk ini hanya ditandai dengan karakteristik semantik tertentu yang dapat menunjukkan jenis kelamin, bentuk tunggal, dan bentuk jamak.

  9. Kandidiasis di Mulut akibat Khemoterapi dan Penatalaksanaannya

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    Sri Budiarti Wonso Hardjono

    2016-10-01

    Full Text Available Latar belakang: kandidiasis (kandidosis adalah infeksi jamur yang disebabkan oleh spesies Candida biasanya Candida albicans. Faktor predisposisi yang memicu kandidiasis adalah terganggunya ekologi mulut karena antara lain pemakaian antibiotika, kortikosteroid, penyakit kronis dan keganasan, beberapa gangguan darah; terapi radiasi di leher dan kepala; khemoterapi; leukimia, obat sitotoksik, juga kebersihan mulut yang buruk. Tujuan: melaporkan kasus kandidiasis di mulut karena khemoterapi dan penatalaksanaannya. Kasus dan penatalaksanaannya: seorang anak laki-laki umur 3 tahun 11 bulan penderita Leukimia Limfoblastik Akut (LLA sedang dalam perawatan khemoterapi menderita kandidiasis di mulut sehingga mengalami disfoni dan disfagia. Makanan dimasukkan lewat hidung. Klinis pasien terlihat lemah infus lewat tangan. Dalam mulut terlihat patch putih tebal menutupi permukaan dorsal dan ventral lidah, palatum durum dan ventral lidah, palatum durum dan molle, mukosa pipi kanan kiri dan gingiva rahang atas dan bawah. Pasien disedasi untuk dilakukan pengerokan lapisan kandida di mulut; pemberian Nystatin solution dan ketokonazol dilanjutkan. Pengerokan psedomembran kandidiasis telah memberikan hasil yang memuaskan, satu minggu kemudian rongga mulut pasien sudah terlihat bersih, pasien sudah dapat makan minum melalui mulut, juga sudah dapat berbicara lagi/tidak serah. Kesimpulan: Pembersihan jamur dengan pengerokan telah dilakukan, kandidiasis pseudomembran dapat diangkat dan mulut pasien sudah bersih, pasien dapat makan dan berbicara lagi.   Background: Candidiasis (candidosis is an fungal infection caused by Candida species usually Candida albicans. Predisposing factors which trigger candidiasis are the ecological disruption caused by use of oral antibiotics, corticosteroids, malignancy and chronic disease; some blood disorders; head and neck radiation; chemotherapy; leukimia, cytotoxic drugs, as well as poor oral hygiene. Aim: To report a case of

  10. Molecular cytogenetics of pituitary adenomas, assessed by FISH technique.

    Science.gov (United States)

    Kontogeorgos, George

    2004-01-01

    Fluorescent in situ hybridization (FISH) represents a moden molecular pathology technique, alternative to conventional cytogenetics (karyotyping). In addition to metaphase spreads, it can be applied directly to interphase nuclei. The latter makes the FISH technique powerful for pathologists for it integrates molecular genetics and classic cytogenetics and brings them together to a single framework for morphologic evaluation. Interphase FISH can be applied to imprints from fresh tissue or to paraffin sections after proteinase K digestion. Centromeric, telomeric and locus DNA-sequence specific probes can be used to identify aneuploidy or gene mutations. Several protocols combine molecular cytogenetics with classic karyotyping. Other sophisticated, FISH-based protocols have been introduced. Among them, comparative genomic hybridization is very important for it can detect non-balanced chromosomal aberrations of uncultured tumor cells and provide overall genomic information in a single experiment. This review presents the principles and applications of FISH technique for the investigation of the cytogenetic background of pituitary adenomas.

  11. PERSEPSI NEGATIF PASIEN KANKER PAYUDARA DAN KOLOREKTAL TERHADAP KEMOTERAPI DAN RADIOTERAPI DI RUMAH SAKIT DI KOTA DENPASAR, BALI

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    Gede Wara Samsarga Samsarga

    2015-01-01

    Full Text Available Terapi adjuvan yang biasanya diberikan pada pasien kanker payudara dan kanker kolorektal adalah kemoterapi dan radioterapi. Terapi adjuvan biasanya diberikan setelah terapi primer untuk meningkatkan angka kesembuhan penyakit, mencegah rekurensi, dan membunuh sel-sel kanker yang tersisa ataupun yang telah bermetastasis (terutama mikrometastasis. Terapi adjuvan juga berfungsi sebagai terapi paliatif untuk meningkatkan harapan hidup pasien kanker. Dalam kenyataannya banyak pasien kanker, khususnya kanker payudara dan kolorektal yang menghindari tindakan kemoterapi dan radioterapi. Dari 38 orang pasien kanker payudara dan kolorektal, didapatkan bahwa 26,3% takut gagal, 39,5% takut efek samping, 7,9% biaya yang mahal, 10,5% karena berlangsung dalam jangka waktu yang lama, dan 15,8% tidak takut terhadap kemoterapi dan radioterapi. Hasil penelitian ini menunjukkan bahwa tingkat pemahaman pasien kanker payudara dan kanker kolorektal terhadap tindakan kemoterapi dan radioterapi masih cukup rendah dimana 68,4% sampel tidak tahu dan tidak mengerti tentang tindakan kemoterapi dan radioterapi. Pemahaman yang kurang tentang tindakan kemoterapi dan radioterapi ini nantinya dapat mengakibatkan timbulnya persepsi negatif terhadap tindakan kemoterapi dan radioterapi. Maka dari itu sangat diperlukan adanya suatu edukasi yang baik bagi setiap pasien tentang penyakit dan modalitas terapi yang akan diberikan. Dokter diharapkan mampu untuk mengubah persepsi negatif pasien terkait tindakan kemoterapi dan radioterapi.  

  12. STATUS POPULASI IKAN NAPOLEON DI WILAYAH TAMAN NASIONAL BUNAKEN DAN KABUPATEN KARAS FAK-FAK

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    Isa Nagib Edrus

    2016-03-01

    Full Text Available Penelitian ikan napoleon di perairan Bunaken pada Oktober 2012 dan Kabupaten Karas pada Nopember 2010 bertujuan untuk mengetahui status populasi ikan tersebut di bawah usaha perlindungan otoritas Taman Nasional Bunaken dan pasca penutupan penangkapannya di Karas.  Metode Underwater Visual Census digunakan untuk mendapatkan data jumlah individu dan ukuran tubuh ikan napoleon.  Hasil penelitian menunjukkan bahwa rata-rata kepadatan ikan napoleon dari luas wilayah sensus 19,26 hektar di Bunaken adalah 1,71 individu per hektar dan dari luas sensus 44,61 hektar di perairan Karas adalah 1,41 individu per hektar. Distribusi panjang frekuensi populasi ikan ini di kedua wilayah tersebut menunjukkan adanya dua kelompok umur, dimana interval ukuran panjang adalah antara 15 cm sampai 100 cm.  Perkembangan populasi ikan tersebut di Bunaken selama 7 tahun menunjukkan peningkatan sebesar 427,5%, sedang di Karas selama kurun waktu 5 tahun sebesar 298%. Namun pertumbuhan populasi sebesar ini ternyata belum masuk pada kategori status kelimpahan yang membaik. Jadi perlindungan ikan ini disarankan untuk lebih diperketat dengan cara melakukan moratorium.   Study on humphead wrasse fish in Bunaken and Karas district waters recpectively in October 2012 and November 2010 were aimed to determine population status of the fish under protecting regulations of the Bunaken National Park Authority and fishing closed session in Karas.  A method used to get population sizes and body sizes was the Napoleon Underwater Visual Census. The results showed that an average of fish density was 1,71 individual/ha of 19.26 ha in area census at Bunaken and it was 1,41 individual/ha of 44.61ha in area census of Karas waters. Distribution frequencies of the fish population were under two cohorts  with body size interval ranged from 15cm to 100 cm. Population trend during seven  years in Bunaken have increased in 427.5 % and those during five years in Karas have increase in 298

  13. FISH applications for genomics and plant breeding strategies in tomato and other Solanaceous crops

    NARCIS (Netherlands)

    Szinay, D.; Bai, Y.; Visser, R.G.F.; Jong, de J.H.

    2010-01-01

    This paper describes the use of advanced fluorescence in situ hybridization (FISH) technologies for genomics and breeding of tomato and related Solanum species. The first part deals with the major determinants of FISH technology: (1) spatial resolution, which depends on the diffraction limit of the

  14. PERANAN LEMAK PAKAN DALAM MENDUKUNG PERKEMBANGAN EMBRIO, DERAJAT PENETASAN TELUR, DAN SINTASAN LARVA IKAN BAUNG (Mystus nemurus

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    Ningrum Suhenda

    2016-11-01

    Full Text Available Penelitian ini dilakukan di Balai Riset Perikanan Budidaya Air Tawar (BRPBAT, Bogor dengan tujuan untuk mengetahui pengaruh kadar lemak pakan yang berbeda terhadap perkembangan embrio, derajat pembuahan, penetasan telur, dan sintasan larva ikan baung. Pakan yang digunakan berupa pakan buatan yang mengandung lemak dengan kadar yang berbeda yaitu 4%, 6%, 8%, 10%, dan 12% dengan sumber lemak yang dipergunakan adalah campuran minyak ikan dan minyak nabati dengan perbandingan 1:1. Bobot rata-rata induk ikan baung pada awal percobaan 420,89±72,10 g/ekor dan dipelihara di dalam kolam beton. Rancangan percobaan yang digunakan yaitu rancangan acak lengkap dengan 5 perlakuan dan 3 ulangan. Parameter yang diamati yaitu derajat penetasan, perkembangan embrio, dan sintasan larva. Hasil penelitian menunjukkan bahwa lemak pakan merupakan faktor penting yang erat hubungannya dengan perkembangan embrio yang terlihat mulai dari pembuahan sampai dengan penetasan yaitu berkisar antara 25 jam 5 menit sampai 26 jam 37 menit. Stadia morula, blastula, dan gastrula terjadi relatif lebih cepat pada induk yang diberi pakan dengan kadar lemak 8%, akan tetapi induk yang diberi pakan dengan kadar lemak 6%, telur yang dihasilkan lebih cepat menetas. Perlakuan kadar lemak 6% dan 8% memperoleh derajat pembuahan di atas 98%, derajat penetasan telur di atas 95%, dan sintasan larva di atas 99%. Selanjutnya pakan ini cukup untuk mendukung terjadinya proses perkembangan embrio secara sempurna dan mendukung sintasan larva. Fish feed should contain sufficient amount of lipid and fatty acid because these elements play eminent roles in supporting reproduction and survival rate of larvae. Fatty acid in egg mass has an important role in fish breeding technology development because it influences the early development of embryogenesis and development of embryo. The research was conducted at the Research Institute for Freshwater Aquaculture, Bogor aimed to study the effect of different

  15. Fish allergy: in review.

    Science.gov (United States)

    Sharp, Michael F; Lopata, Andreas L

    2014-06-01

    Globally, the rising consumption of fish and its derivatives, due to its nutritional value and divergence of international cuisines, has led to an increase in reports of adverse reactions to fish. Reactions to fish are not only mediated by the immune system causing allergies, but are often caused by various toxins and parasites including ciguatera and Anisakis. Allergic reactions to fish can be serious and life threatening and children usually do not outgrow this type of food allergy. The route of exposure is not only restricted to ingestion but include manual handling and inhalation of cooking vapors in the domestic and occupational environment. Prevalence rates of self-reported fish allergy range from 0.2 to 2.29 % in the general population, but can reach up to 8 % among fish processing workers. Fish allergy seems to vary with geographical eating habits, type of fish processing, and fish species exposure. The major fish allergen characterized is parvalbumin in addition to several less well-known allergens. This contemporary review discusses interesting and new findings in the area of fish allergy including demographics, novel allergens identified, immunological mechanisms of sensitization, and innovative approaches in diagnosing and managing this life-long disease.

  16. Do Fish Resist?

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    Dinesh Joseph Wadiwel

    2016-03-01

    Full Text Available There have been a number of scientific studies on the question of whether fish feel pain. Some have suggested that some fish indeed do feel pain and that this has significant welfare implications (2003. Others have argued that fish do not have the brain development necessary to feel pain. In terms of number of animals killed, the slaughter of sea animals for human consumption significantly exceeds that of any land animals that we use for food, and sea animal slaughter practices frequently lack any basic welfare protections. If fish can be shown to feel pain—or more importantly, if humans can agree that fish feel pain—then this would place a significant question mark over many contemporary fishing practices.  This article substitutes the question 'Do Fish Feel Pain?' with an alternative: 'Do Fish Resist?' It explores the conceptual problems of understanding fish resistance, and the politics of epistemology that surrounds and seeks to develop a conceptual framework for understanding fish resistance to human capture by exploring the development of fishing technologies - the hook, the net and contemporary aquaculture.

  17. Fish under exercise.

    Science.gov (United States)

    Palstra, Arjan P; Planas, Josep V

    2011-06-01

    Improved knowledge on the swimming physiology of fish and its application to fisheries science and aquaculture (i.e., farming a fitter fish) is currently needed in the face of global environmental changes, high fishing pressures, increased aquaculture production as well as increased concern on fish well-being. Here, we review existing data on teleost fish that indicate that sustained exercise at optimal speeds enhances muscle growth and has consequences for flesh quality. Potential added benefits of sustained exercise may be delay of ovarian development and stimulation of immune status. Exercise could represent a natural, noninvasive, and economical approach to improve growth, flesh quality as well as welfare of aquacultured fish: a FitFish for a healthy consumer. All these issues are important for setting directions for policy decisions and future studies in this area. For this purpose, the FitFish workshop on the Swimming Physiology of Fish ( http://www.ub.edu/fitfish2010 ) was organized to bring together a multidisciplinary group of scientists using exercise models, industrial partners, and policy makers. Sixteen international experts from Europe, North America, and Japan were invited to present their work and view on migration of fishes in their natural environment, beneficial effects of exercise, and applications for sustainable aquaculture. Eighty-eight participants from 19 different countries contributed through a poster session and round table discussion. Eight papers from invited speakers at the workshop have been contributed to this special issue on The Swimming Physiology of Fish.

  18. M. Quraish Shihab dan Rasionalisasi Tafsir

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    Afrizal Nur

    2012-06-01

    Full Text Available Kesempurnaan Islam adalah karena bersumberkan kepada al-Qur’an dan Sunnah, kedua sumber ini melahirkan peraturan atau popular dengan istilah syari’at. Islam menuntut umat nya untuk mendalami dan menghayati ayat-ayat yang terkandung didalam kitab suci al-Qur’an karena mempunyai maksud tersendiri, maka oleh karena nya tafsir mengambil peranan yang strategis untuk memahami dan mengungkap rahasia keagungan khazanah pada setiap ayat al-Qur’an. Berdasar motifasi tinggi untuk memahami al-Qur’an, banyak ulama di Indonesia yang berkosentrasi untuk menjelaskan dan menafsirkan al-Qur’an, dalam sejrah aktifitas penafsiran al-Qur’an berawal dari Syeikh Abdurra’uf al-Singkli pada abad ke 17M sampai saat ini adalah era nya M.Quraish Shihab. Tokoh tafsir yang terakhir ini tidak pernah absent dan ketinggalan di kaji dan di analisa oleh para komunitas akademisi tafsir baik dalam bentuk kajian kritik ataupun mendukung pencapaian prestasi kitab tafsir yang diberi nama al-Mishbah ini. Kritikan yang selalu dapat perhatian utama akademisi tafsir meliputi pandangan-pandangan rasional Quraish Shihab yang sering kali menjadi tidak rasional disebabkan tidak mengikuti dan menyalahi pandangan jumhur ulama. Oleh sebab itu penulis tidak ingin ketinggalan menyumbangkan pemikiran dalam bentuk usaha intelektual alah kadarnya yang dimiliki untuk menempatkan tafsir al-Mishbah kemartabat yang tinggi di tangga samudera karya tafsir ulama tafsir Indonesia

  19. FAKTOR PERSONAL DAN SITUASIONAL PENERIMAAN PESAN DAKWAH

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    Enung Asmaya

    2017-02-01

    Full Text Available The da’wa messages are not necessarily able to subdue mad’u. Subjugation and readiness of mad’u is influenced by personal and situational factors which are always intertwined in giving rulings behavior. Personal factors is surrounding the biological and sociopsychological aspects. Biological aspects such as needs and aspects sociopsychological human basic form of the condition and cognitive, affective and psychomotor of mad’u. The situational factors may include environmental systems, culture, norms, architectural design and the like. Social situation is also part of the reason for mad’u to accept or reject the message of da’wa.   Pesan dakwah tidak serta-merta dapat menundukkan mad’u. Penundukan dan kesiapan mad’u dipengaruhi faktor personal dan situasional yang senantiasa berkelindan dalam memberikan putusan-putusan perilaku. Faktorfaktor personal melingkupi aspek biologis dan sosiopsikologis. Aspek biologis berupa kebutuhan-kebutuhan dasar manusia dan aspek sosiopsikologis berupa kondisi dan kemampuan kognitif, afeksi dan konasi mad’u. Adapun faktor situasional dapat berupa sistem lingkungan, budaya, norma, design arsitektur dan sejenisnya. Kondisi sosial juga menjadi alasan mad’u dalam menerima atau menolak pesan dakwah.

  20. APA DAN MANA DALAM KALIMAT DEKLARATIF

    Directory of Open Access Journals (Sweden)

    Sri Puji Astuti

    2016-06-01

    Full Text Available Kalimat merupakan salah satu sarana untuk menyampaikan maksud pembicara atau penulis kepada pendengar atau pembaca. Untuk menyampaikan informasi digunakan kalimat deklaratif. Dalam kalimat deklaratif sering ditemui kata apa dan mana serta bentuk turunannya. Bagaimana kehadiran apa dan mana serta bentuk turunannya dalam kalimat deklaratif dibahas dalam makalah ini. Teori yang digunakan untuk menganalisis adalah teori stuktural. Pengumpulan data dilakukan dengan cara menyimak pemakaian bahasa. Dalam analisis data digunakan metode distribusional yaitu metode analisis data yang berupa penghubungan aantarfenomena dalam bahasa tertentu itu sendiri  Adapun teknik yang d.igunakan dalam penelitian ini adalah teknik delisi, substitusi, dan teknik parafrasa. Dari hasil penelitian disimpilkan  bahwa apa dan mana serta bentuk tuturannya jika terdapat dalam kalimat deklaratif bukan merupakan interogativa. Kehadiran apa dan mana serta bentuk tuturannya dalam kalimat deklaratif ada yang bersifat wajib ada yang bersifat opsional. Apa dan mana serta bentuk turunannya  bersifat opsional jika dalam kalimat deklaratif apa dan mana serta bentuk tuturannya diikuti atau didahului oleh kata yang mempunyai makna yang sama

  1. HASIL ANALISIS VITAMIN A DAN β-KAROTEN BAHAN MAKANAN SUMBER VITAMIN A DAN KAROTEN DENGAN METODE HPLC

    Directory of Open Access Journals (Sweden)

    Yuniar Rosmalina

    2012-11-01

    Full Text Available Telah dilakukan analisis vitamin A DAN β-Karoten terhadap beberapa jenis pangan dengan menggunakan metode HPLC. Analisis dilakukan terhadap 9 jenis serealia dan umbi-umbian, 3 jenis kacang-kacangan, 40 jenis sayuran, 11 jenis daging dan hasilnya, 7 jenis telur dan hasilnya, 8 jenis ikan, 12 jenis buah-buahaan, dan 7 jenis kelompok lain-lain. Hasilnya menunjukkan sumber β-Karoten yang tinggi pada kelompok serealia dan umbi-umbian adalah umbi jalar, pada kelompok sayuran adalah daun katuk, daun pepaya, daun singkong, daun melinjo, daun talas dan daun sintrong, pada kelompok telur adalah telur bebek, pada kelompok buah-buahan adalah mangga golek dan mangga gedong. Sedangkan kelompok kacang-kacangan dan kelompok daging kandungan β-Karotennya rendah. Hati ayam, hati bebek, dan hati kambing merupakan sumber vitamin A yang tinggi, sedangkan pada kelompok ikan didapati ikan lele mempunyai kandungan vitamin A yang lebih tinggi dibandingkan jenis ikan lainnya.

  2. Sistem Komputerisasi Pengolahan Buku Induk Pegawai Pada UPT Dinas Pendidikan Pemuda Dan Olahraga Kecamatan Banjarmangu Berbasis PHP Dan MYSQL

    Directory of Open Access Journals (Sweden)

    Elisa Usada

    2011-11-01

    Full Text Available Permasalahan yang akan dibahas adalah bagaimana membuat sistem pengolahan buku induk pegawai di UPT Dinas Pendidikan Pemuda dan Olahraga Kecamatan Banjarmangu berbasis PHP dan MySQL. Sistem pengolahan buku induk pegawai  berbasis PHP dan MySQL ini dirancang komputerisasi menggunakan PHP dan database MySQL dengan tujuan agar pengolahan data pegawai di UPT Dinas Pendidikan Pemuda dan Olahraga Kecamatan Banjarmangu dapat terpusat. Metode penelitian yang digunakan adalah menggunakan metode waterfall. Sistem pengolahan buku induk pegawai ini menggunakan jaringan client server dan dapat diakses oleh administrator dan Kepala UPT dan Pegawai. Kelebihan dari sistem pengolahan ini adalah dilindungi password dan hak akses yang berbeda untuk setiap user sehingga keamanan data dapat terjamin, sedangkan kekurangannya adalah program yang dihasilkan belum maksimal karena mengkhususkan data pegawai pada buku induk saja. Saran dari sistem pengolahan ini yaitu pengembangan dari segi keamanan data, kelengkapan data, dan ketelitian sistem dalam input data untuk mengurangi kesalahan.

  3. Zoonoses associated with fish.

    Science.gov (United States)

    Boylan, Shane

    2011-09-01

    The taxonomic group that composes the fishes is the most diverse group of vertebrates worldwide. The challenges of unique physiologies, a foreign environment, and many unknowns attract a passionate group of biologists and veterinarians. Economically, fishes have become vital as food, bait, and companion animals. Fishermen and fish handlers (processing plants) represent the historical human population exposed to fish zoonoses, but growth in aquaculture and aquarium hobbyists have led to an increase in published fish-borne zoonotic cases starting in the late 1950s that bloomed in the 1980s. Human physicians, particularly dermatologists and infectious disease specialists, are now more aware of fish-borne zoonoses, but they can be assisted with diagnosis when informed patients give more detailed histories with fish/water exposure.

  4. Comparison Between Fluorescent In Situ Hybridization (FISH) and ...

    African Journals Online (AJOL)

    ... were collected, for culture method and bacteria characterized by biochemical ... for 24 hours and later preserved in 70% alcohol before in situ hybridization test. ... were observed in 47 to 75% while the bacterium was isolated on culture in 7 ...

  5. Perancangan dan Implementasi Presensi Digital Guru dan Karyawan SMA Negeri 9 Semarang

    Directory of Open Access Journals (Sweden)

    Th. Didot Septiawan

    2016-04-01

    Full Text Available Berdasarkan surat edaran dari Kepala Dinas Pendidikan Kota Semarang, terhitung mulai Januari 2015 tiap sekolah dihimbau untuk beralih dari sistem presensi manual menjadi digital. Hingga awal Maret ini, presensi guru dan karyawan di SMA Negeri 9 Semarang masih dilakukan secara manual. Maka diperlukan sebuah penelitian untuk merancang dan menerapkan sistem presensi digital bagi SMA Negeri 9 Semarang. Tahapan penelitian meliputi perencanaan, analisis, perancangan, implementasi, pengujian, dan pemeliharaan. Perangkat lunak dibangun dengan framework PHP Phalcon, basis data MySQL, dan aplikasi webserver Nginx pada sistem operasi Ubuntu 14.04. Mesin presensi dengan metode pengenalan wajah dipilih untuk mendapatkan data kehadiran yang bersifat autentik. Pengujian dilakukan dengan metode kotak hitam dan kotak putih. Semua kebutuhan fungsional telah diimplementasikan pada aplikasi web dan kebutuhan non-fungsional pun telah terpenuhi. Sistem presensi digital ini menjadi solusi bagi SMA Negeri 9 Semarang dalam usaha uji coba penggunaan presensi digital.

  6. TINJAUAN UMUM TENTANG HELPDESK DAN FRAMEWORK TERKAIT

    Directory of Open Access Journals (Sweden)

    Ali Tarmuji

    2013-04-01

    Full Text Available Penggunaan teknologi informasi dan komunikasi (TIK di suatu instansi sangat banyak dampaknya, baik positif maupun negatif. Seiring perkembangan teknologi semakin banyak pemanfaatan media TIK ini untuk membantu pelaksanaan suatu pekerjaan bahkan hampir seluruh bidang di suatu instansi. Dalam perjalanan penggunaan TIK sering muncul permasalahan-permasalahan yang jika tidak ditangani akan mengakibatkan adanya kemunduran kinerja dari bagian/seseorang yang menggunakan peralatan TIK untuk menunjang kerja mereka. Suatu instansi yang sudah sedemikian kompleksnya dalam penggunaan peralatan TIK di instansinya maka diperlukan suatu mekanisme dan unit khusus yang menangani penggunaan dan permasalahan dalam hal penggunaan TIK tersebut. Mekanisme dan unit khusus tersebut sering dinamakan tim helpdesk yang tujuan utamanya adalah menangani penyelesaian terhadap permasalahan yang muncul dari penggunaan suatu peralatan TIK tersebut sehingga mampu memperlancar kerja yang terkait. Suatu instansi dalam hal penerapan tim helpdesk memerlukan suatu perencanaan yang matang dan terorganisir. Langkah-langkah yang perlu dilakukan oleh instansi dalam implementasi tim helpdesknya antara lain, (1 membentuk tim khusus untuk menganalisis organisasinya terkait kebutuhan implementasi helpdesk tersebut, (2 membentuk organisasi helpdesk sesuai kebutuhan, (3 memilih workflow helpdesk yang disesuaikan dengan kondisi instansi, (4 memilih framework yang tepat untuk mendasari jalannya implementasi helpdesk di instansi terkait, dan (5 menuangkan aturan-aturan dasar (standard operation procedur-SOP untuk menguatkan kiprah tim helpdesk di instansi terkait.Terbentuknya tim helpdesk diharapkan dapat mengatasi permasalahan dan memberikan layanan terhadap seluruh permasalahan bidang TIK baik yang bersifat internal (pengembang aplikasi maupun seluruh permasalahan operasional aplikasi ataupun hal-hal yang berhubungan dengan sistem dan teknologi informasi dan komunikasi.

  7. KEMUNCULAN DAN TINGKAH LAKU PESUT (Orcaella brevirostris (Owen in Gray 1866 SEBAGAIMAMALIATERANCAMLANGKA DI PERAIRANKUBU RAYADANKAYONGUTARAKALIMANTAN BARAT

    Directory of Open Access Journals (Sweden)

    Regi Fiji Anggawangsa

    2015-12-01

    2013. Selama pengamatan hanya diketahui terdapat satu kelompok pesut sebanyak 4-6 individu yang terdiri dari pesut muda dan dewasa. Kelompokan itu dijumpai di muara Sungai Bumbun pada kedalamanan perairan 11 meter. Tingkah laku yang teramati menunjukkan gerombolan pesut umumnya memburu kelompokan ikan dan sesekali menyemburkan air dari blowhole nya. Berdasarkan ciri-ciri morfologi dan tingkah laku yang teramati serta kondisi lingkungan perairan setempat maka perairan di Kabupaten Kubu Raya dan Kabupaten Kayong Utara yang terletak di Provinsi Kalimantan Barat diduga merupakan perairan yang potensial sebagai habitat pesut (Orcaella brevirostris. Irrawaddy dolphins (Orcaella brevirostris (Owen in Gray 1866 is one of aquatic mammals species who populations are increasingly threatened. The lack information about population of Irrawaddy dolphins in West Kalimantan waters makes the conservation of this species and its management were still not optimized yet. This research aims to identify the characteristics and behavior of dolphins found in Kubu Raya and Kayong Utara waters of West Kalimantan. The observation was held on April 2013, exploring coastal waters and estuaries which are expected to be the habitats of Irrawaddy dolphins using binoculars. There are 4-6 individuals of Irrawaddy dolphin found at the mouth of Bumbun River. A group consist of young and adults dolphins at the depth of 11 meters. The observed behavior is chasing schooling fish and occasionally spitting water from his blowhole. Based on morphological character and fish behavior observed, and environmental condition parameters, indicated that the water surrounding of Kubu Raya and Kayong Utara District were potential habitat for Irrawaddy dolphins.

  8. Penskalaan Butir Format Respons Pilihan dan Respons Bebas Berdasarkan Model Rasch dan Partial Credit

    Directory of Open Access Journals (Sweden)

    Eko Hariadi

    2007-06-01

    Full Text Available Penelitian melihat pengaruh jumlah parameter butir, kategori respons bebas (RB, pengaruh sampel terhadap akurasi estimasi parameter kemampuan untuk menghasilkan estimasi yang stabil dan pengaruh pembobotan butir RP dan butir RB terhadap kesalahan baku. Penelitian dalam dua tahap, simulasi menggunakan 30 kondisi dengan replikasi 50 dengan variabel panjang tes, jumlah kategori, dan jumlah parameter butir, dan analisis deskriptif, dilanjutkan penerapan penskalaan gabungan butir tipe respons pilihan (rp dan butir respons bebas (rb pada konstruksi tes elektronika yang terdiri 40 butir pilihan ganda dan 4 butir jawaban tersusun, 3 butir memiliki lima kategori jawaban dan 1 butir dengan 4 kategori jawaban, melibatkan 355 siswa. Hasil penelitian menunjukkan: ukuran sampel kurang berpengaruh pada root mean square error atau (RSME> dan korelasi antara 9 dengan 0, namun berpengaruh terhadap akurasi estimasi parameter butir pilihan ganda (/>/y,dan parameter butir respons tersusun (3^- Jumlah parameter butir berpengaruh terhadap parameter kemampuan, tetapi tidak berpengaruh terhadap akurasi dari b^, dan S„,. Estimasi dari parameter tingkat kesulitan butir jawaban tersusun tiga kategori lebih akurat daripada butir jawaban tersusun lima kategori. Estimasi tahan {robust untuk parameter kesulitan butir jawaban tersusun 5 kategori memerlukan sampel minimal 250 responden, sedangkan untuk butir respons tersusun 3 kategori memerlukan sampel minimal 100 responden. Estimasi parameter kemampuan dari skor total (0^^ tidak sama dengan rata-rata jumlah tbeta dari masing-masing subtes (0^ + 0CR. Theta dari tes yang dikalibrasi bersama-sama berbeda dengan theta dari total subtes yang dikalibrasi secara terpisah. Korelasi kemampuan yang mengunakan pembobotan dan kemampuan tanpa pembobotan mempunyai suatu rentang dari 0,988 sampai 0,948. Kata kunci: penyekaiaan, model rash dan partial credit.

  9. Evaluasi Layanan Sirkulasi dan Layanan Pengguna Pada Kantor Perpustakaan, Kearsipan dan Dokumentasi Nias Utara

    OpenAIRE

    Zalukhu, Radius Seantero

    2016-01-01

    Tujuan penelitian ini adalah untuk mengetahui layanan perpustakaan pada kantor perpustakaan, kearsipan dan dokumentasi kabupaten Nias Utara. Penelitian yang dilakukan di Kantor Perpustakaan, Kearsipan dan Dokumenstasi Kabupaten Nias Utara, yang beralamat di Jl. Gunung Sitoli – Lehewa Km 42 Lotu, menitik beratkan pada layanan sirkulasi, layanan pengguna serta layanan koleksi. Metode Penelitian menggunakan metode deskriptif. Instrument yang digunakan dalam penelitian ini adalah kuesioner dan st...

  10. ANEMIA DAN ANEMIA GIZI BESI PADA KEHAMILAN: HUBUNGANNYA DENGAN ASUPAN PROTEIN DAN ZAT GIZI MIKRO

    OpenAIRE

    Nur Handayani Utami; Bunga CH. Rosha

    2015-01-01

    Anemia masih menjadi permasalahan kesehatan pada wanita hamil. Zat besi dianggap sebagai salah satu zat gizi mikro yang berperan terhadap terjadinya anemia. Kekurangan gizi besi dalam tingkat lanjut dapat menyebabkan anemia, yang disebut sebagai anemia gizi besi. Tujuan studi ini adalah untuk menganalisis perbedaan antara asupan protein dan gizi mikro serta menghitung odd ratio (OR) kejadian anemia dan anemia gizi besi akibat asupan protein dan gizi mikro pada wanita hamil di lokasi studi. An...

  11. Status Oral Higiene Dan Periodontal Pada Pasien Diabetes Melitus Dan Non- Diabetes Di RSUD Dr. Pirngadi

    OpenAIRE

    Butar-Butar, Mardiah Rizqo

    2014-01-01

    Diabetes melitus adalah penyakit gangguan metabolisme ditandai dengan meningkatnya kadar gula darah yang terjadi karena kelainan sekresi insulin, kerja insulin, atau kedua-duanya. Diabetes melitus merupakan salah satu penyakit sistemik yang berperan sebagai faktor risiko penyakit periodontal. Penelitian ini bertujuan untuk mengetahui status oral higiene dan periodontal dan odd rasio periodontitis pada pasien Diabetes melitus dan non-Diabetes. Rancangan penelitian adalah case-control. Popul...

  12. Pencahayaan Buatan pada Pendekatan Teknis dan Estetis untuk Bangunan dan Ruang Dalam

    OpenAIRE

    Budi Setiawan; Grace Hartanti

    2014-01-01

    Cahaya merupakan hal yang penting bagi manusia dan makhluk hidup lainnya. Tanpa cahaya atau penerangan tentu sulit untuk beraktivitas, terutama saat hari menjelang malam. Seiring berkembangnya peradaban dan teknologi, manusia menciptakan pencahayaan buatan. Awalnya, pencahayaan buatan sebagai pengganti sinar alami matahari, lalu cahaya buatan yang didapat dari api, kemudian penelitian dan pengembangan bola lampu oleh Alessandro Volta, Sir Humphry Davy, Warren De La Rue, hingga Tho...

  13. Perencanaan Pemerintah Kabupaten Kudus Dalam Mempersiapkan Pengalihan Pajak Bumi dan Bangunan Sektor Perdesaan dan Perkotaan Sebagai Pajak Daerah

    Directory of Open Access Journals (Sweden)

    Vitriana Masfita

    2013-08-01

    Full Text Available Penelitian ini dilatarbelakangi oleh terbitnya Undang-undang Nomor 28 Tahun 2009 tentang Pajak Daerah dan Retribusi Daerah, Pemerintah Pusat mengalihkan kewenangan pengelolaan Pajak Bumi dan Bangunan sektor Perdesaan dan Perkotaan kepada Pemerintah Daerah. Oleh karena itu, Pemerintah Daerah harus mempersiapkan segala keperluan agar proses pengalihan ini berjalan dengan lancar. Tujuan penelitian ini adalah untuk mendeskripsikan dan menganalisis (1 rencana pemerintah Kabupaten Kudus dalam persiapan pengambilalihan Pajak Bumi dan Bangunan Sektor Perkotaan dan Perdesaan, (2 faktor-faktor yang mempengaruhi persiapan Pemerintah Kabupaten Kudus dalam pengalihan Pajak Bumi dan Bangunan Sektor Perkotaan. Penelitian ini merupakan penelitian kualitatif deskriptif dengan menggunakan data-data kualitatif berupa kata-kata, tindakan dan dokumen. Teknik pengumpulan data yang digunakan adalah interview dengan Kepala DPPKD Kabupaten Kudus beserta Kepala Bidang, Kepala Seksi dan Staff, observasi partisipasi di DPPKD Kabupaten Kudus, dan studi dokumentasi dokumen kedinasan di DPPKD Kabupaten Kudus. Data diperoleh melalui Analisis data menggunakan model Miles dan Huberman. Hasil Penelitian: (1 Pemerintah Kabupaten kudus berencana untuk mempersiapkan Sarana dan Prasarana yang meliputi penyediaan ruang pelayanan, ruang server dan pengolahan data, pengadaan perlengkapan gedung kantor, pengadaan software dan hardware komputer, pengadaan barang cetakan; membentuk UPT Pelayananan Pajak Daerah; mempersiapkan personel dibidang IT, pelayanan pajak daerah, administrasi pajak daerah; membentuk Peraturan Daerah dan Peraturan Bupati terkait Pajak Bumi dan Bangunan; menyiapkan SOP; menjalin kerjasama dengan KPP Pratama dan Bank Jateng; membuka rekening PBB P2 di Bank Jateng.  (2 Faktor pendukung dari proses perencanaan Pemerintah Kabupaten Kudus adalah: tersedianya dana atau pembiayaan, partisipasi SKPD terkait, serta partisipasi dari Kantor Pelayanan Pajak Pratama dan Bank

  14. DIAMETER SERAT DAN PANJANG SARKOMER DAGING SAPI BALI HASIL PENGGEMUKAN MENGGUNAKAN PAKAN DENGAN LEVEL KULIT BIJI KAKAO DAN OTOT BERBEDA

    OpenAIRE

    2015-01-01

    2015 NUR AMALIA (I111 11 271). Diameter Serat dan Panjang Sarkomer Daging Sapi Bali Hasil Penggemukan Menggunakan Pakan dengan Level Kulit Biji Kakao dan Otot Berbeda. Dibawah bimbingan Hikmah M. Ali sebagai pembimbing utama, dan Ratmawati Malaka sebagai pembimbing anggota. Penelitian ini bertujuan untuk mengetahui pengaruh jenis otot, level kulit biji kakao dalam pakan, dan interaksi keduanya terhadap panjang sarkomer dan diameter serat daging sapi Bali. Penelitian ini ...

  15. Southeast Alaska ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains biological resource data for estuarine, benthic, and pelagic fish in Southeast Alaska. Vector polygons in this data set represent locations of...

  16. Columbia River ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and freshwater fish species in Columbia River. Vector polygons in this...

  17. North Slope, Alaska ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and freshwater fish species for the North Slope of Alaska. Vector...

  18. American Samoa ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for reef, pelagic, benthic, and estuarine fish species in American Samoa. Vector polygons in this data set...

  19. IMPLEMENTASI MATRIKS PADA MATEMATIKA BISNIS DAN EKONOMI

    Directory of Open Access Journals (Sweden)

    Yuniarsi Rahayu

    2012-05-01

    Full Text Available Penelitian ini membahas tentang analisis masukan-keluaran yang merupakan salah satu penerapan  matriks dan sebagai model matematika untuk menganalisis struktur perekonomian yang saling berhubungan antara kegiatan ekonomi. Matematika penting sekali untuk dipelajari dan dikuasai, dikarenakan suatu kasus membutuhkan pemahaman yang berbentuk matematis yaitu pemodelan matematika. Metode yang digunakan adalah Metode Invers Matriks dan Metode Eliminasi Gauss-Jordan. Perhitungan ini menggunakan alat bantu Matlab (matrix laboratory yang memungkinkan untuk menangani kalkulasi matematis dengan cara yang mudah. Kata kunci : Matriks, Metode, Input-Output

  20. Media Sosial dan Perkembangan Komunikasi Korporat

    Directory of Open Access Journals (Sweden)

    Rina Juwita

    2017-07-01

    Full Text Available Kehadiran media sosial telah mengubah aktivitas komunikasi korporat dan dengan cepat merevolusi bagaimana kampanye atau program public relations berjalan. Jika dibandingkan dengan cara tradisional yang mengandalkan pada output murni, media sosial memaksa komunikasi korporat beralih pada proses dialog dimana para pemangku kepentingan, dan bukan hanya perusahaan sama-sama memiliki kekuasaan terhadap pesan yang beredar. Media sosial merupakan alat komunikasi revolusioner yang mengubah secara cepat bagaimana praktik PR menjadi bagian integral dari komunikasi korporat bagi sejumlah perusahaan dan menawarkan pilihan baru bagi para praktisi PR pada setiap aspek proses komunikasi yang dilakukan.

  1. Prinsip Transparansi dalam Perjanjian TBT dan SPS

    Directory of Open Access Journals (Sweden)

    Dina Widyaputri Kariodimedjo

    2012-02-01

    Full Text Available Transparency principle in the TBT and SPS Agreement is implemented by notification. Its application is prepared by related agencies through the making of regulations, establishment of enquiry points, building capacity and infrastructure. It guarantees certainty, predictability and credibility of Indonesia in international trade, and its application should not undermine national trade policy. Prinsip transparansi dalam Perjanjian TBT dan SPS diterapkan melalui notifikasi. Penerapan prinsip ini dilakukan oleh instansi-instansi terkait melalui pembuatan peraturan, upaya terkait kelembagaan, SDM dan infrastruktur. Prinsip yang bertujuan menciptakan kepastian, konsistensi bagi perdagangan dan kredibilitas Indonesia dalam perdagangan internasional, harus dilakukan tanpa mengorbankan kepentingan untuk mengamankan kebijakan impor nasional.

  2. Sugar Cane Genome Numbers Assumption by Ribosomal DNA FISH Techniques

    NARCIS (Netherlands)

    Thumjamras, S.; Jong, de H.; Iamtham, S.; Prammanee, S.

    2013-01-01

    Conventional cytological method is limited for polyploidy plant genome study, especially sugar cane chromosomes that show unstable numbers of each cultivar. Molecular cytogenetic as fluorescent in situ hybridization (FISH) techniques were used in this study. A basic chromosome number of sugar cane

  3. Determinan Kemiskinan dan Pengangguran di Jawa Tengah

    Directory of Open Access Journals (Sweden)

    Medha Wardhany

    2017-08-01

    Full Text Available Masalah kemiskinan dan pengangguran merupakan masalah pokok yang dihadapi  secara nasional dan regional bahkan juga secara global, yang perlu mendapat perhatian serius. Besarnya angka kemiskinan dan pengangguran dalam upaya penanggulangannya memerlukan berbagai informasi dan kajian agar penanggulangan tersebut dapat berjalan secara efektif dan efisien. Penelitian ini bertujuan untuk mendapatkan informasi dan hasil yang dapat dijalankan dalam program-program penanggulangan kemiskinan dan pengangguran secara lebih efektif dan efesien sesuai dengan harapan seluruh pihak termasuk masyarakat yang masih dikategorikan sebagai marginal. Penelitian ini menggunakan sumber data utamanya berasal dari data sekunder yakni Susenas dan Sakernas untuk data Makro serta terkait lainnya seperti data Supas, dan SP 2010, ST2013 dan PBDT 2015 untuk data mikro serta data PODES untuk data pendukung kewilayahan. Metode analisis dalam penelitian ini adalah analisis deskriptif dan eksploratif dari berbagai data dan informasi yang tersedia. Selain itu, juga akan dilakukan beberapa perhitungan model statistik yang relevan dengan kajian ini. Dari hasil analisis regresi linier berganda kemiskinan diperoleh informasi bahwa dari uji F dengan α=0,05 diperoleh nilai signifikansi sebesar 0,000. Artinya, variabel yang dimasukkan ke dalam model secara simultan berpengaruh terhadap tingkat kemiskinan. Sedangkan secara parsial variabel status penguasaan bangunan, Luas Lantai per Kapita, Jenis Lantai dan rasio jumlah kamar, Bahan Bakar memasak rumah tangga, aset, Status pekerjaan KRT keluarga miskin, jam kerja, Penerimaan Program (KPS dan Jumlah Bangunan di permukiman kumuh berpengaruh signifikan terhadap kemiskinan. Dari hasil pengolahan analisis regresi menunjukkan nilai signifikansi dari variabel pengangguran kurang dari 0,05. Hal ini sesuai dengan teori yang menyatakan bahwa secara simultan variabel independen tamat, penyakit dan jumlah industri berpengaruh signifikan terhadap

  4. PENGKAJIAN PROGRAM KURSUS DAN PELATIHAN TERKAIT DENGAN JENIS KETERAMPILAN, SERTIFIKASI DAN PENEMPATAN LULUSAN

    Directory of Open Access Journals (Sweden)

    Melati Indri Hapsari

    2016-02-01

    Full Text Available Penelitian ini bertujuan mengidentifikasi jenis-jenis keterampilan yang dibutuhkan di dunia usaha dan industri terutama pasar ASEAN yang telah tersedia lembaga sertifikasinya, mengetahui pelaksanaan sertifikasi kompetensinya, dan proses penempatan tenaga kerja yang akan bekerja di luar negeri. Lokasi penelitian dilakukan di BNSP, BKSP, Dinas Tenaga Kerja dan Transmigrasi, serta BP3TKI. Subyek dipilih sesuai kompetensinya. Penelitian deskriptif kualitatif ini menggunakan teknik pengumpulan data dengan wawancara, dokumentasi dan studi pustaka. Keabsahan data menggunakan kriteria kredibilitas, dependabilitas, konfirmabilitas, dan transferabilitas. Simpulan penelitian ini adalah bahwa jenis-jenis keterampilan yang dibutuhkan di dunia usaha dan industri terutama pasar ASEAN antara lain pariwisata, operator produksi, kayu lapis, konstruksi. Yang telah siap MRA-nya adalah bidang pariwisata dengan dikeluarkannya ACCSTP dan CATC. Pelaksanaan sertifikasi kompetensi di Indonesia adalah Lembaga Sertifikasi Profesi yang telah mendapat lisensi dari Badan Nasional Sertifikasi Profesi. Proses penempatan tenaga kerja yang akan bekerja di luar negeri hanya dapat dilakukan oleh pemerintah dan pelaksana penempatan TKI swasta. Prosedur penempatan dimulai dari pra penempatan, penempatan dan pasca penempatan.

  5. EKSISTENSI DAN SEBARAN NYAMUK AEDES AEGYPTI DAN AEDES ALBOPICTUS DI KAMPUS UNIVERSITAS HASANUDDIN MAKASSAR

    Directory of Open Access Journals (Sweden)

    Yosefina Dota T

    2015-03-01

    Full Text Available Penelitian yang bertujuan untuk mengetahui eksistensi dan sebaran nyamuk Aedes aegypti dan Aedes albopictus telah dilakukan di Kampus Universitas Hasanuddin, Kec. Tamalanrea, Makassar. Penelitian bersifat eksploratif dengan melakukan sampling terhadap lima lokasi yaitu : a Fak. Peternakan (Utara, b Fak. Hukum (Timur, c Pusat Kegiatan Penelitian/PKP (Selatan, d Workshop/Dekat Pondokan mahasiswa (Barat dan e Fak. MIPA (Tengah. Sampling nyamuk menggunakan metode ovitrap (menggunakan attraktan Eluisine Indica L. dan survei terhadap berbagai tempat penampungan air. Sampel telur dan larva nyamuk yang diperoleh disimpan dalam microtube berisi alkohol 70% kemudian diidentifikasi berdasarkan Rueda (2004. Hasil penelitian menunjukkan bahwa Nyamuk Ae. aegypti dan Ae. albopictus ditemukan hidup dan berkembang biak di kampus Universitas Hasanuddin, Makassar. Eksistensi dan sebaran kedua jenis nyamuk tersebut dipengaruhi oleh faktor adanya manusia/masyarakat kampus yang beraktivitas baik di dalam ruangan (indoor maupun di luar ruangan (outdoor, adanya berbagai tempat penampungan air baik buatan (bak mandi, ember maupun barang bekas (botol/kaleng bekas, tempurung kelapa, vegetasi/tanaman dan berbagai macam hewan yang berada di sekitaran kampus. Hasil penelitian dalam ruangan (indoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di dalam ruangan gedung PKP sedangkan Ae.albopictus lebih banyak di Fak. Hukum. Hasil penelitian di luar ruangan (outdoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di area Workshop sedangkan Ae.albopictus lebih banyak di area PKP.

  6. Fish elevator and method of elevating fish

    Science.gov (United States)

    Truebe, Jonathan; Drooker, Michael S.

    1984-01-01

    A means and method for transporting fish from a lower body of water to a higher body of water. The means comprises a tubular lock with a gated entrance below the level of the lower body of water through which fish may enter the lock and a discharge passage above the level of the upper body of water. The fish raising means in the lock is a crowder pulled upward by a surface float as water from the upper body of water gravitationally flows into the closed lock filling it to the level of the upper body. Water is then pumped into the lock to raise the level to the discharge passage. The crowder is then caused to float upward the remaining distance through the water to the level of the discharge passage by the introduction of air into a pocket on the underside of the crowder. The fish are then automatically discharged from the lock into the discharge passage by the out of water position of the crowder. The movement of the fish into the discharge passage is aided by the continuous overflow of water still being pumped into the lock. A pipe may be connected to the discharge passage to deliver the fish to a selected location in the upper body of water.

  7. Learning the scientific method using GloFish.

    Science.gov (United States)

    Vick, Brianna M; Pollak, Adrianna; Welsh, Cynthia; Liang, Jennifer O

    2012-12-01

    Here we describe projects that used GloFish, brightly colored, fluorescent, transgenic zebrafish, in experiments that enabled students to carry out all steps in the scientific method. In the first project, students in an undergraduate genetics laboratory course successfully tested hypotheses about the relationships between GloFish phenotypes and genotypes using PCR, fluorescence microscopy, and test crosses. In the second and third projects, students doing independent research carried out hypothesis-driven experiments that also developed new GloFish projects for future genetics laboratory students. Brianna Vick, an undergraduate student, identified causes of the different shades of color found in orange GloFish. Adrianna Pollak, as part of a high school science fair project, characterized the fluorescence emission patterns of all of the commercially available colors of GloFish (red, orange, yellow, green, blue, and purple). The genetics laboratory students carrying out the first project found that learning new techniques and applying their knowledge of genetics were valuable. However, assessments of their learning suggest that this project was not challenging to many of the students. Thus, the independent projects will be valuable as bases to widen the scope and range of difficulty of experiments available to future genetics laboratory students.

  8. Inovasi Desain, Teknologi, dan Pemasaran Lewat Website Usaha Kecil Menengah Batik dan Lutik (Lurik Batik di Kecamatan Laweyan Surakarta

    Directory of Open Access Journals (Sweden)

    Anastasia Riani Suprapti

    2016-12-01

      Tujuan penelitian terapan ini adalah untuk meningkatkan produktivitas dan mengembangkan produk batik dan lutik yang ada di wilayah Kecamatan Laweyan Surakarta dengan inovasi desain dan teknologi baru. Program ini bekerja sama dengan mitra UKM Batik Dewi (UKM 1 dan Batik Sinung Rejeki (UKM2 yang terletak di Kecamatan Laweyan. Pengusaha batik Laweyan dengan produknya berupa batik dan tekstil bermotif batik (printing dan cap dalam era globalisasi sekarang ini ternyata memiliki daya tahan yang baik, ditengah persaingan dengan produk sejenis dari daerah lain maupun dari luar negeri. Secara garis besar pola yang digunakan dalam implementasi penelitian ini meliputi: diskusi (FGD, kerja bengkel, operasional pabrik, pelatihan dan pendampingan (kelompok dan individu. Seperti halnya Usaha Kecil Menengah pada umumnya, pengusaha batik di Laweyan juga menghadapi masalah Internal meliputi  permodalan, inovasi desain, pemasaran, dan manajemen keuangan, yang meliputi administrasi keuangan/pembukuan. Beberapa solusi yang ditawarkan untuk mengatasi permasalahan yang dihadapi diantaranya adalah melakukan Inovasi desain dan teknologi, pengaturan layout pabrik, dan pemasaran lewat web. Selain itu mengembangkan desain motif batik yang lebih diminati pasar diberikan juga untuk pengembangan teknik kombinasi batik-lurik ikat dengan cara lukis dan pengelantangan. Penggunaan media teknologi informasi dan komunikasi untuk promosi lewat website. Adapun untuk usaha pengembangan manajemen pemasaran,  diusahakan media promosi dan menjalin kerjasama pemasaran  dengan instansi terkait, misalnya dengan mengikuti pameran-pameran di Solo dan Jakarta.

  9. VARIASI KUALITAS AIR DAN ESTIMASI POTENSI PRODUKSI IKAN PERAIRAN DANAU BATUR, PROPINSI BALI

    Directory of Open Access Journals (Sweden)

    Samuel Samuel

    2016-03-01

    Full Text Available Danau Batur merupakan tipe danau vulkanik berbentuk kaldera di Propinsi Bali yang tidak mempunyai aliran air keluar (outlet. Danau ini mempunyai peranan penting sebagai sumber penyediaan protein hewani bagi masyarakat Kabupaten Bangli khususnya dan Bali pada umumnya. Informasi mengenai kondisi limnologi dan biologi perairan yang dapat digunakan sebagai dasar untuk pengembangan dan pengelolaan perikanan belum banyak tersedia. Oleh karena itu, penelitian mengenai aspek limnologi dan estimasi potensi produksi ikan dilakukan pada Februari, Mei, September dan Nopember 2011.  Estimasi potensi produksi ikan diketahui dengan menerapkan rumus empiris dari hubungan antara produksi ikan dengan indeks morfoedafik. Dari nilai indeks status trofik perairan (55,17–61,14, perairan Danau Batur diklasifikasikan sebagai perairan dalam tingkat eutofik ringan sampai sedang. Dari nilai-nilai parameter daya hantar listrik, konsentrasi fosfat (PO4-P, nitrat (NO3-N, total nitrogen (TN, dan alkalinitas, perairan Danau Batur secara umum dapat dikategorikan sebagai perairan subur yang dapat dimanfaatkan oleh organisme air yang berfungsi sebagai makanan alami, yang dapat mendukung kehidupan ikan. Perairan Danau Batur yang relatif subur ini memberikan gambaran potensi produksi sebesar 98,56-99,48 kg/ha/tahun (rata-rata 99,15 kg/ha/tahun. Dengan memperhitungkan luas perairan danau, potensi ikan Danau Batur diperkirakan secara rata-rata sebesar 159 ton/tahun. Nilai potensi produksi ini relatif lebih tinggi dari target produksi perikanan pada 2010 sebesar 150 ton. Agar dapat mempertahankan keberlanjutan proiduksi perikanan tangkap di Danau Batur, disarankan agar produksi perikanan tidak melebihi nilai potensi produksi seperti yang diperoleh dalam penelitian ini. Lake Batur is a type of volcanic lake shaped caldera that has no water flow out (outlet. The lake has an important role to provide a protein source for the people of Bangli regency. However, the Information about the

  10. The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon

    OpenAIRE

    Sparks, John S.; Robert C. Schelly; W. Leo Smith; Matthew P Davis; Dan Tchernov; Pieribone, Vincent A.; Gruber, David F.

    2014-01-01

    The discovery of fluorescent proteins has revolutionized experimental biology. Whereas the majority of fluorescent proteins have been identified from cnidarians, recently several fluorescent proteins have been isolated across the animal tree of life. Here we show that biofluorescence is not only phylogenetically widespread, but is also phenotypically variable across both cartilaginous and bony fishes, highlighting its evolutionary history and the possibility for discovery of numerous novel fl...

  11. PRODUKTIVITAS ARMADA PENANGKAPAN DAN POTENSI PRODUKSI PERIKANAN UDANG DI LAUT ARAFURA

    Directory of Open Access Journals (Sweden)

    Purwanto Purwanto

    2016-03-01

    Full Text Available Tulisan ini menyajikan hasil kajian potensi produksi dan upaya penangkapan optimal serta menjelaskan perkembangan perikanan udang di Laut Arafura, termasuk pula estimasi dampak dari peningkatan upaya penangkapan terhadap produktivitas kapal dan hasil tangkapan udang. Berdasarkan hasil analisis, potensi produksi udang penaeid yang dapat dihasilkan secara lestari dari pemanfaatan stok udang di Laut Arafura adalah 49,5 ribu ton/tahun dengan upaya penangkapan sekitar 635 unit kapal pukat udang 130 GT. Walaupun upaya penangkapan dari kapal yang memiliki surat izin penangkapan ikan di Laut Arafura tahun 2011 lebih rendah dibandingkan upaya penangkapan optimal untuk menghasilkan MSY, stok udang penaeid tersebut telah dimanfaatkan melebihi tingkat optimum akibat tingginya intensitas operasi kapal perikanan tanpa izin. Estimasi kerugian hasil tangkapan akibat kegiatan penangkapan ikan ilegal juga disajikan disini.   This paper presents result of the assessment of potential production and optimal fishing effort, and briefly describes the past development of the shrimp fishery in the Arafura Sea, including estimated impact of increasing fishing pressure on the vessel productivity and the quantity of catch. Based on the result of analysis, the maximum sustainable yield from the utilisation of the penaeid shrimp stock in the Arafura Sea was about 49,500 tonnes/year resulting from the operation of 635 units of shrimp trawlers. Although fishing effort from vessels granted license in 2011 was lower than the optimum level to produce MSY, the penaeid shrimp stock was overexploited as the intensity of illegal fishing practices by unlicensed vessels was high.The estimate of catch losses caused by illegal fishing is also presented here.

  12. 荧光原位杂交技术在血尿患者膀胱尿路上皮癌筛查中的应用%Detection of bladder cancer from the urine using fluorescence in situ hybridization(FISH) technique

    Institute of Scientific and Technical Information of China (English)

    江长琴; 梁朝朝; 叶元平; 刘明; 张贤生; 郝宗耀; 周骏; 樊松

    2010-01-01

    目的 评价荧光原位杂交(fluorescence in situ hybridization,FISH)技术在血尿患者膀胱尿路上皮癌筛查中的临床应用价值,为从尿液脱落细胞中早期诊断膀胱癌提供新的途径.方法 选择20例健康正常人,通过FISH技术检测9号染色体p16位点、3号染色体、7号染色体及17号染色体变异情况,建立阈值.100例血尿患者留取晨尿,分别进行尿脱落细胞学检查和FISH技术检查.统计分析FISH方法的敏感度和特异度.结果 探针检测p16基因完全缺失阈值为3.9%,部分缺失为5.4%,扩增为3.2%;3号染色体缺失阈值为4.0%.扩增为3.5%;7号染色体缺失阈值为4.0%,扩增为2.8%;17号染色体缺失阈值为5.8%,扩增为3.4%.以至少两种探针检测结果超过阈值或一种探针检测结果至少存在两种异常为诊断阳性.FISH技术在血尿患者膀胱尿路上皮癌筛查中的敏感度和特异度分别为93.6%和100%,尿脱落细胞学检测分别为46.8%和100%.结论 FISH技术无创快速,对血尿患者膀胱尿路上皮癌筛查的敏感度优于尿脱落细胞学检查方法.

  13. Dan Marino Helping Those with Autism Spectrum Disorders | NIH MedlinePlus the Magazine

    Science.gov (United States)

    ... Dan Marino Foundation. Photo: Dan Marino Foundation Former NFL star quarterback Dan Marino and his wife Claire ... have to succeed in life,'" says the former NFL star. "That's where the focus for The Dan ...

  14. DESAIN KOMUNIKASI VISUAL DAN PERILAKU KONSUMEN

    Directory of Open Access Journals (Sweden)

    Bagus Limandoko

    2000-01-01

    Full Text Available The word design is a word with different meanings. In the visual communication context it has became part of the team in the communication industry - the world of advertising%2C magazine and newspaper publishing%2C marketing and public relations%2C and%2C indeed%2C it has became one of the influential aspects to shape the behavior of a society and its economic development. Abstract in Bahasa Indonesia : Desain merupakan sebuah kata dengan banyak makna. Dalam konteks komunikasi visual%2C desain sudah menjadi bagian dari tim dalam industri komunikasi. Dunia advertising%2C publikasi majalah dan suratkabar%2C pemasaran dan public relations%2C dan yang pasti desain juga sudah menjadi salah satu aspek yang berpengaruh dalam membentuk perilaku suatu masyarakat dan perkembangan ekonominya. design%2C designer%2C visual communication design%2C behavior%2C consumer

  15. Sintesa dan Karakteristik Sifat Mekanik Karet Nanokomposit

    Directory of Open Access Journals (Sweden)

    Pocut Nurul Alam

    2007-06-01

    Full Text Available Peningkatan sifat mekanik karet alam dengan penambahan tanah liat nanokomposit pada kosentrasi berbeda yaitu 1, 3 dan 5 % berat sudah berhasil diteliti. Pada percobaan ini pengujian dilakukan dengan X-Ray Difraction (X-RD untuk analisa morphologi dan Instron untuk analisa uji tarik. Penambahan tanah liat nanokomposit kedalam matrik polimer adalah untuk meningkatkan sifat mekanik dari material asli dan juga untuk menghasilkan suatu produk polimer yang lebih murah. Hasil yang diperoleh adalah terjadinya peningkatan yang drastis terhadap basal spacing dari matrik polimer dan menunjukkan intercalasi diantara polimer dengan pengisinya. Uji tarik juga menununjukkan peningkatan yang sangat signifikan yaitu 14.983 MPa pada karet alam menjadi 40.178 MPa pada karet alam-tanah liat nanokomposit 5% berat. Kata kunci: karet alam, sifat mekanik, tanah liat nanokomposit

  16. DAN-IODP-SEIS Project

    DEFF Research Database (Denmark)

    Bendixen, Carina; Boldreel, Lars Ole; Jensen, Jørn Bo

    The DAN-IODP-SEIS is aimed at investigating the geological development of Kattegat during the last 130.000 years. This will be based on acoustic (seismic) methods and geological interpretation of cores from previously drilled shallow wells and the IODP wells drilled in 2013. A large number of ref...... Denmark and is a collaboration project between IGN, GEUS and IGG. Further information regarding the cruise that took place in June-July will be available in issue no. 3 2013 of Geoviden........ The data will be integrated with already existing archive data. The data is to be processed and interpreted using state of the art interpretation software (Petrel). Seismic examples are shown to illustrate aspects of the geological development of the Kattegat. This PhD project is funded by Geocenter...

  17. Fluorescence antibunching microscopy

    CERN Document Server

    Schwartz, Osip

    2011-01-01

    Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent markers, routinely used as contrast labels for bio-imaging. The technique can be readily implemented using standard fluorescence microscopy equipment.

  18. Fluorescence of atopic allergens

    NARCIS (Netherlands)

    Berrens, L.

    1967-01-01

    Purified atopic allergens have been found to emit flue fluorescence upon irradiation with ultraviolet light of 365 mμ wavelength. The maximum of fluorescence is in the region 445–490 mμ and the intensity is of the same order of magnitude for different atopic allergens. Synthetic model compounds, inc

  19. Fluorescent Lamp Replacement Study

    Science.gov (United States)

    2017-07-01

    Light-emitting diode, LED, lighting, fluorescent, waste reduction, energy conservation, net zero , mercury 16. SECURITY CLASSIFICATION OF: 17. LIMITATION...Center (ATC) to assess the benefits of converting fluorescent tube lighting to light-emitting diode (LED) technology. The report documents the waste ...1-15 SECTION 2. SUBTESTS 2.1 HAZARDOUS WASTE REDUCTION

  20. LEDs for fluorescence microscopy

    NARCIS (Netherlands)

    Young, I.T.; Garini, Y.; Dietrich, H.R.C.; Van Oel, W.; Liqui Lung, G.

    2004-01-01

    Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light

  1. Penurunan Kadar Amonium dan Fosfat pada Limbah Cair Industri Pupuk

    Directory of Open Access Journals (Sweden)

    Aulia Rodlia Fitriana

    2017-01-01

    Full Text Available Limbah cair industri pupuk mengandung kadar amonium dan fosfat yang cukup tinggi. Kadar ini dihasilkan dari kegiatan produksi pabrik amoniak dan pabrik asam fosfat. Penurunan ammonium dan fosfat ini dapat dilakukan melalui proses presipitasi kimiawi dan menghasilkan mineral Magnesium Ammonium Phosphate (MAP atau Struvite. Sumber Mg yang akan digunakan adalah MgCl2. Penelitian dilakukan dalam skala laboratorium menggunakan sistem batch. Variabel yang digunakan adalah kecepatan pengadukan, pH, dan rasio molar [Mg2+]:[NH4+]:[PO43-]. pH yang akan digunakan adalah 7,5; 8; dan 8,5. Sedangkan rasio molar yang digunakan adalah 1:1:1; 1,5:1:1; dan 2:1:1. Kedua hasil variabel ini merupakan hasil dari permodelan Visual MINTEQ v3.0. Variasi G.td yang digunakan adalah 0,5.106; 106; dan 1,5.106. Presipitasi dilakukan selama 60 menit dan dilanjutkan dengan proses sedimentasi selama 30 menit. Hasil yang diperoleh pada penelitian ini adalah presentase removal amonium dan fosfat optimum terjadi pada pH 8,5 dan rasio molar 1:1:1 dengan G.td 106 atau setara degan 158 rpm. Kadar amonium awal sebesar 2864 mg/L dan fosfat sebesar 14656 mg/L. Residual amonium sebesar 396,82 mg/L dan residual fosfat sebesar 148,86 mg/L. Residual amonium dan fosfat pada penelitian ini masih tinggi.

  2. PERMASALAHAN DAN POTENSI PESISIR DI KABUPATEN SAMPANG

    Directory of Open Access Journals (Sweden)

    Aries Dwi Siswanto

    2016-03-01

    Full Text Available SAMPANG’S COASTAL PROBLEMS AND POTENTIALS One area that has progressive economic value and environmental degradation possibility is a dynamic areas of coastal areas. These characteristics is interesting in the perspective of management and utilization of marine resources and fisheries. The rapid development of the Sampang regency required the government to focus and provide greater attention to minimize the ecological pressures that affect the carrying capacity of the environment, particularly in coastal areas. This study aims to gather information about the problem and the potential that exists in coastal areas of Sampang. Data taken by interviewing respondents lived in coastal communities during April 2015 and the results of the questionnaire were analyzed descriptively. The problems identified include land requirement, the overlapping use, the threat of pollution and environmental degradation, and zoning; whereas the potential that exists include salt, marine tourism, mariculture, and the development of industrial areas and ports. The potential problems identified in coastal areas and beaches in Sampang relatively identical to the Bangkalan. It needs a comprehensive solution to minimize the existing impact as well as efforts to optimize utilization to achieve integrated coastal zone management.Keywords: coastal, CZM, problem, potential, Sampang ABSTRAKSalah satu wilayah yang memiliki nilai ekonomi progresif sekaligus peluang degradasi lingkungan sehingga disebut daerah dinamis adalah wilayah pantai dan pesisir. Karakteristik ini menjadi sesuatu yang menarik dalam perspektif pengelolaan dan pemanfaatan sumberdaya kelautan dan perikanan. Pesatnya perkembangan tersebut menuntut pemerintah kabupaten Sampang untuk focus dan memberikan perhatian lebih besar sebagai upaya untuk meminimalkan tekanan ekologis yang berpengaruh terhadap daya dukung lingkungan, khususnya di wilayah pesisir. Penelitian ini bertujuan untuk mengumpulkan informasi

  3. BY PRODUCT TERNAK. Teknologi dan Aplikasinya

    OpenAIRE

    Said, Muhammad Irfan

    2014-01-01

    DAFTAR PUSTAKA Adventini, N., Muhayatun, D. D. Lestiani dan W.Y.N. Syahfitri. 2012. Analisis Mikronutrien dalam Daging, Limpa, dan Hati Sapi. Jurnal Medika. 38 (8), 580???586. Angelidaki, I., and B. K. Ahring. 2000. Methods for increasing the biogas potential from the recalcitrant organic matter contained in manure. Water Science Technology. 41 (3), 189-194. Anonim. 1974. Gelita-Instant-Gelatins Type 800 A Type 800 B. Gelatine-Information-Service. Deutsche Gelatine-Febrike...

  4. La guitare dans les musiques traditionnelles

    OpenAIRE

    Rougier, Thierry

    2002-01-01

    International audience; Ce que l'organologie classique désigne sous le nom de "guitare" connaît en fait une très grande variété (de forme, d'accord, de mode de jeu) dans les musiques traditionnelles. L'ethnomusicologie rend compte de la continuité entre les cordophones de type guitare, les autres cordophones et les instruments à percussion, sur de nombreux terrains (dans la musique flamenca, brésilienne...)

  5. PRINSIP DAN KRITERIA PERIKLANAN DARI PERSPEKTIF ISLAM

    Directory of Open Access Journals (Sweden)

    Teuku Meldi Kesuma

    2012-06-01

    Full Text Available Advertising in marketing Islamic products must be believed and understood as an important part of marketing based on Islam which should not be separated. This paper examines the principles and criteria of the advertising in the perspective of Islam. It is caused by the increasing of the companies and institutions which are based on Islamic values such as Islamic financial institutions that promote its products the consumers. The important issues discussed in this paper is how the real of concepts, principles, and criteria of Islamic advertising? Therefore this paper is developing an advertising concept from the perspective of Islam and to know for sure whether the principles and criteria that should exist in advertising Islam. The methodology applied in this paper is through literature review by using content analysis. Hopefully, this study could develop an Islamic perspective advertisement concept in terms of principles and criteria. =========================================== Periklanan dalam memasarkan produk-produk Islam harus diyakini dan di pahami merupakan bahagian penting dalam pemasaran yang berdasarkan Islam yang tidak boleh dipisahkan. Tulisan ini mengkaji prinsip-prinsip dan kriteria periklanan dalam perspektif Islam. Hal ini disebabkan semakin ramai dan tingginya pertumbuhan perusahaan dan institusi yang berlandaskan nilai-nilai Islam seperti institusi keuangan Islam yang mempromosikan produk dan perkhidmatannya kepada orang ramai. Persoalan penting yang dibahas dalam kertas kerja ini adalah bagaimanakah bentuk konsep, prinsip dan kriteria periklanan Islam yang sebenarnya? Oleh karena itu tujuan kertas kerja ini adalah untuk membangun satu konsep periklanan dari perspektif Islam dan untuk mengenal pasti apakah prinsip-prinsip serta kriteria yang seharusnya ada dalam periklanan Islam. Metodologi kajian yang digunakan dalam kertas kerja ini adalah metode kepustakaan dengan menggunakan analisis isi. Kajian ini diharapkan dapat

  6. PENGEMBANGAN ALAT PERAGA SEDERHANA STRUKTUR DAN ORGAN DALAM IKAN UNTUK MEMPERMUDAH PEMBELAJARAN PADA PRAKTIKUM IKHTIOLOGI PERIKANAN

    Directory of Open Access Journals (Sweden)

    Arief Budiyanto

    2015-10-01

    Full Text Available Simple structure and organs model of fish used to help students collage in observing fish organ structure in Ichtiology practicum. In fact, there are many observers found difficulties to learn fish organs structure. During the practicum, there are several part of organs that cannot be clearly seen or too small. Moreover, it happened for the fishes that has small size. Another, If the organ structure is not in a good condition it will be difficult to be observed. As a replecement, observer can see dan observe by using tool model to see inside organs of fish. Beside, it make PJMK/Assistant to explain inside part of fish organ structure. Simple structure and organs model of fish is validated by academic civitas that have expertised in fisheries. The result was very worthy to be used in learning process in inchtiology practicum. Score that has been gotten is 100 %. In the class observation, it included 100 observer of Ichtiology. The result of the questionair are: the interesting of fish shape model is 72% was very satisfied.  Model, tool design, accessories and completeness of tool model is 70% was satisfied. The beneficial of the ichtiology praticum is 80% was helped. The corellation of tool and praticum material ichtiology 80%. The suggestion from the tool user in the future, 96%, suggested to use the tool model. Observer comments that mastery assitant in the used of the tool model 65% still less. 70% observer stated that the tool is still need improvement.Keywords: ichtiology practicum, observer, tool model

  7. Dimensi Agama dan Reaksi Dunia Melayu Terhadap Penetrasi Barat di Abad XVIII dan XIX Khasnya di Riau, Melaka, Palembang, dan Aceh

    OpenAIRE

    Alfian, Ibrahim

    2013-01-01

    Tulisan ini mengangkat "kisah" dua kitab Dala'il al-Khairat dan Nasihat al-Muslimin yakni dua aspek ajaran Islam shalwat kepada Nabi Besar Muhammad saw. dan panggilan jihad sabilillah di dunia Melayu abad 18 dan 19 yang dapat memberikan kita beberapa hal penting.

  8. Do Fish Sleep?

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    Most fish can’t shut their eyes, so it’s easy to think they don’t sleep. But that’s like assuming humans don’t sleep because we can’t shut our ears to drown out sound. In fact, many species of fish take time out during the day or (more often) at night to enter a sleeplike stage. Some of these fish float in place, others lie on the bottom。

  9. Fish and wildlife surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Poston, T.M.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the monitoring of radioactive contaminants in fish and wildlife species that inhabit the Colombia River and Hanford Site. Wildlife have access to areas of the Site containing radioactive contamination, and fish can be exposed to contamination in spring water entering the river along the shoreline. Therefore, samples are collected at various locations annually, generally during the hunting or fishing season, for selected species.

  10. Kontestasi Kekuasaan dan Keteladanan Semu di Indonesia

    Directory of Open Access Journals (Sweden)

    Michael HB Raditya

    2016-04-01

    Full Text Available Kepemimpinan di Indonesia merupakan persoalan yang menarik untuk ditelaah. Jika bertolak dari tataran diakronis, secara kronologis Indonesia telah mengalami pelbagai macam fase kepemimpinan dan rezim. Ihwal ini dapat menjadi stimulan positif jika masyarakat dapat mengartikulasikan pengalaman bernegaranya dengan tepat. Kontestasi kekuasaan pun tidak dapat terhindarkan, terlebih setiap pemimpin melakukan persaingan dalam memperebutkan kekuasaan. Kontestasi ini tidak hanya merujuk pada tingkat lokal, namun hingga tingkat nasional. Berbagai aspek pun turut menjadi faktor utama dari kontestasi tersebut, seperti tipe kepemimpinan aristokrasi dan demokrasi, hingga perbedaan gender pemimpinnya. Ihwal tersebut turut mengejewantah kan bahwa keteladanan yang didasarkan atas beberapa kontekstual, bersifat pseudo, atau semu. Dalam mengupas ihwal tersebut, telaah yang digunakan merujuk pada pemahaman Barkerakan agen, dan Gramsci akan hegemoni. Tidak hanya itu, Kouzes dan Posner digunakan untuk menilik parameter keteladanan. Asumsi penulis, keteladanan merupakan unsur yang tercipta untuk menghegomoni agen di dalamnya. Bertolak dari mempertanyakan keteladanan, maka pembahasan akan lebih mempertimbangkan aspek agen, hegemoni dan kuasa. Penulis menyadari bahwa tidak mungkin meneliti semua pola kepemimpinan yang ada di Indonesia, maka rujukan contoh kepemimpinan didasarkan pada beberapa contoh di era kolonial, orde lama, orde baru,dan pemerintahan kini. Penelitian ini mencoba untuk mengupas persoalan keteladanan dalam kepemimpinan yang kerap diunggulkan kepemimpinan yang ada di Indonesia.

  11. Pembuatan dan Karakteristik Komposit Polimer Berpenguat Bagasse

    Directory of Open Access Journals (Sweden)

    Eqitha Dea Clareyna

    2013-09-01

    Full Text Available Bagasse memiliki kandungan serat yang cukup besar dan berpotensi digunakan sebagai bahan penguat dalam pembuatan komposit karena sifatnya yang kuat dan ringan. Dalam penelitian tugas akhir ini telah dibuat bahan komposit berpenguat bagasse dengan empat macam ukuran penguat yaitu serat chopped serta partikel berukuran 100 mesh, 140 mesh, dan 200 mesh. Pembuatan komposit menggunakan metode hand lay-up dan fraksi volume penguat divariasi dari 2,5% hingga 15%. Hasil karakterisasi yang telah dilakukan, menunjukkan bahwa kekuatan tarik dan densitas terbaik dimiliki oleh sampel komposit dengan penguat berukuran 200 mesh sebanyak 7,5% volume. Kekuatan tarik sampel tersebut adalah  28,83  MPa dan densitasnya adalah 1,15 gr/cm3. Adapun kekuatan impak terbesar dimiliki oleh sampel komposit dengan 2,5% volume sebesar 0,00271 J/mm2. Dengan demikian sampel komposit yang telah dibuat dapat digunakan sebagai alternatif bahan baku industri menggantikan tiang penyangga (scantlings pada struktur kayu (timber structure sesuai standar AS 1720.1.

  12. Al-Farabi dan Filsafat Kenabian

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    Qosim Nursheha Dzulhadi

    2014-03-01

    Full Text Available Artikel ini mengulas pemikiran al-Farabi tentang konsep kenabian dalam Islam. Masalah berawal dari adanya pemikiran yang menolak konsep kenabian pada umumnya dan kenabian Nabi Muhammad SAW khususnya. Sebagaimana yang dilontarkan oleh Muhammad Ahmad ibn al-Ruwandi. Ia mengatakan bahwa nabi sebenarnya tidak diperlukan manusia karena Tuhan telah mengaruniakan akal kepada manusia tanpa terkecuali. Dengan akal ini, manusia dapat mengetahui Tuhan beserta segala nikmat Nya dan dapat pula mengetahui perbuatan baik dan buruk, menerima suruhan dan larangan-Nya. Dengan demikian, nabi dengan segala fungsinya tidak diperlukan lagi. Bahkan, kitab suci pun tidak berguna untuk dibaca. Lebih berguna membaca buku filsafat Epicurus, Plato, Aristoteles, dan buku astronomi, logika, serta obat-obatan. Hal ini tentunya bertolak belakang dengan yang diajarkan dalam Islam. Beriman kepada nabi nabi merupakan hal inti dalam ajaran agama ini. Atas dasar itu al-Farabi bereaksi keras. Baginya, pandangan Ibn al-Ruwandi di atas tidak dapat dibenarkan, khususnya dari sisi akidah Islam. Sebagai al-Mu‘allim al-Tsani (Guru Kedua, karena prestasinya dalam menjelaskan dan mengulas-ulang filsafat Aristoteles, al-Farabi mengkritik secara sistematik pandangan menyimpang al-Ruwandi di atas.

  13. KEBIJAKAN PEMBANGUNAN KELAUTAN DAN PERIKANAN KE DEPAN DEVELOPMEN POLICY OF MARINE AND FISHERIES

    Directory of Open Access Journals (Sweden)

    Sharif Cicip Sutardjo

    2014-05-01

    Full Text Available Konsep negara kepulauan sangat besar manfaatnya bagi Indonesia, karena dapat menjadikan laut sebagai penyatu pulau-pulau serta memperluas wilayah perairan kita. Sumber daya kelautan Indonesia merupakan wilayah Marine Mega-Biodiversity terbesar di dunia, dengan potensi sumber daya ikan tangkap laut sebesar 6,5 juta ton per tahun, potensi perikanan budidaya payau mencapai 2,96 juta hektar dan potensi budidaya laut yang mencapai luasan 12,55 juta hektar. Selain itu laut Indonesia memiliki potensi migas dan wisata yang besar. Hasil pembangunan kelautan dan perikanan telah dapat meningkatkan PDB perikanan menjadi 6,48 % dengan nilai nominal sebesar Rp 57,69 triliun dan meningkatkan ekspor dengan surplus sebesar US$ 3,52 milyar. Selain itu juga dapat meningkatkan produksi perikanan, tingkat konsumsi ikan, produk olahan dan surplus produksi garam. Luas kawasan konservasi juga meningkat tajam, begitu juga pengelolaan pulaupulau kecil dan pengawasan. Keberhasilan pembangunan perikanan tersebut tercapai berkat program industrialisasi perikanan dengan pendekatan ekonomi biru. Kebijakan pembangunan ke depan adalah kebijakan pembangunan yang berkelanjutan (sustainable development dengan mempertimbangkan konsep negara kepulauan dan potensi kelautan yang besar.   Concept of archipelagic country gives a big benefit for Indonesia, since the concept introduces the sea as unifying islands and widensour territorial waters. Indonesian marine resources are the largest marine mega-biodiversityin the world, in which the potencies for capture fisheries resources, brackishwater aquaculture area, and marine culture area are 6.5 millions/year,2.96 million ha and 12.55 million ha respectively. Meanwhile, the sea has also huge potency for oil and gas as well as tourism. Marine and fisheries development has increased GDP of fisheries into 6.48% which is approximately valued Rp 57.69 trilliun and increased export surplus of US$ 3.52 billion. The development has also increased

  14. Combating Illegal Fishing

    Directory of Open Access Journals (Sweden)

    Sorin Stanciu

    2010-10-01

    Full Text Available Illegal, unreported and unregulated (IUU fishing is a worldwide phenomenon. Its extent and its environmental,economic and social consequences are such that it has become a priority issue at international level. IUU fishingcontributes to the depletion of fish stocks and jeopardises protection and recovery measures put in place to ensure theviability of resources. It represents unfair competition for those who exploit fish resources legally. The Commissionhave been involved in the fight against IUU fishing for over a decade and in 2002 the Commission adopted an ActionPlan against IUU fishing inspired by the FAOs International Plan of Action to prevent, deter and eliminate IUUfishing of 2001. However, despite regional and international efforts to stop IUU fishing the phenomenon is still agrowing problem and as a result, the European Community intensified its action towards IUU fishing by launching aconsultation process in 2007. A Proposal to prevent, deter and eliminate IUU fishing was adopted in October 2007and a Regulation to prevent, deter and eliminate illegal, unreported and unregulated (IUU fishing was adopted on 29September 2008, after a unanimous political agreement.

  15. Anglers' fishing problem

    CERN Document Server

    Karpowicz, Anna

    2011-01-01

    The considered model will be formulated as related to "the fishing problem" even if the other applications of it are much more obvious. The angler goes fishing. He uses various techniques and he has at most two fishing rods. He buys a fishing ticket for a fixed time. The fishes are caught with the use of different methods according to the renewal processes. The fishes' value and the inter arrival times are given by the sequences of independent, identically distributed (i.i.d.) random variables with the known distribution functions. It forms the marked renewal--reward process. The angler's measure of satisfaction is given by the difference between the utility function, depending on the value of the fishes caught, and the cost function connected with the time of fishing. In this way, the angler's relative opinion about the methods of fishing is modelled. The angler's aim is to have as much satisfaction as possible and additionally he has to leave the lake before a fixed moment. Therefore his goal is to find two...

  16. Of Fish and Micrornas

    DEFF Research Database (Denmark)

    Bela-Ong, Dennis; Schyth, Brian Dall; Lorenzen, Niels

    Fish is an important small vertebrate multidisciplinary model for investigating various aspects of reproduction, development, disease (immunology, toxicology, carcinogenesis), and aging. It is also an important model for comparative and evolutionary studies because it represents the lower...... to the mechanisms of control of gene expression, impacting a broad range of biological processes. Thus far, >25, 000 miRNA sequences have been identified in 193 species, including fish. In fish, the interest on miRNAs started with the analysis of their expression and function during embryonic development. In our...... selection markers to identify disease-resistant fish....

  17. Intelligent Fish Freshness Assessment

    Directory of Open Access Journals (Sweden)

    Hamid Gholam Hosseini

    2008-01-01

    Full Text Available Fish species identification and automated fish freshness assessment play important roles in fishery industry applications. This paper describes a method based on support vector machines (SVMs to improve the performance of fish identification systems. The result is used for the assessment of fish freshness using artificial neural network (ANN. Identification of the fish species involves processing of the images of fish. The most efficient features were extracted and combined with the down-sampled version of the images to create a 1D input vector. Max-Win algorithm applied to the SVM-based classifiers has enhanced the reliability of sorting to 96.46%. The realisation of Cyranose 320 Electronic nose (E-nose, in order to evaluate the fish freshness in real-time, is experimented. Intelligent processing of the sensor patterns involves the use of a dedicated ANN for each species under study. The best estimation of freshness was provided by the most sensitive sensors. Data was collected from four selected species of fishes over a period of ten days. It was concluded that the performance can be increased using individual trained ANN for each specie. The proposed system has been successful in identifying the number of days after catching the fish with an accuracy of up to 91%.

  18. PENGGUNAAN TUTOR SEBAYA UNTUK PENINGKATAN AKTIVITAS DAN HASIL BELAJAR

    Directory of Open Access Journals (Sweden)

    Rakimahwati -

    2014-06-01

    Abstrak: Penggunaan Tutor Sebaya untuk Peningkatan Aktivitas dan Hasil Belajar. Penelitian ini bertujuan untuk meningkatkan aktivitas dan hasil belajar mahasiswa dengan menerapkan metode tutor sebaya pada perkuliahan Perencanaan Pembelajaran TK. Metode yang digunakan dalam penelitian adalah penelitian tindakan kelas. Pelaksanaan tindakan dilakukan dua siklus terhadap mahasiswa reguler Pendidikan Guru Pendidikan Anak Usia Dini (PG-PAUD Universitas Negeri Padang. Data diperoleh melalui observasi, angket dan penilaian akhir kegiatan. Data kuantitatif dianalisis dengan rerata dan persentase, dan dikom­parasikan dengan data kualitatif. Hasil penelitian menunjukkan bahwa penggunaan metode tutor sebaya dapat meningkatkan aktivitas dan hasil belajar mahasiswa.

  19. RUMAH TRADISIONAL OSING KONSEP RUANG DAN BENTUK

    Directory of Open Access Journals (Sweden)

    Iwan Suprijanto

    2002-01-01

    , Banyuwangi-Jawa Timur, yang secara historis-genealogis-sosiologis masih memperlihatkan tata kehidupan tradisional dan memiliki rumah Osing relatif banyak. Tujuan penelitian adalah memperoleh konsep ruang dan bentuk pada rumah tradisional Osing di Desa Kemiren dan faktor-faktor yang melatarbelakanginya. Hasil penelitian adalah : 1. Konsep Ruang Rumah Osing Pola ruang menganut susunan ruang Bale, Jrumah dan Pawon secara berurut dari depan ke belakang. Organisasi ruang menganut prinsip closed ended plan. Konsep ruang memperlihatkan adanya dualitas dan centralitas. 2. Konsep Bentuk Rumah Osing Bentuk atap merupakan indikator utama bentuk rumah Osing, yang dapat dibedakan menjadi Tikel Balung, Baresan dan Cerocogan. Karakteristik bentuk rumah Osing terletak pada penggunaan 1, 2 atau 3 bentuk dasar tersebut secara sekaligus dalam susunan berurut dari depan ke belakang sesuai dengan susunan ruangnya. 3. Faktor-faktor yang melatarbelakangi Konsep masing-masing ruang dipengaruhi oleh fungsi dan aktivitas, makna kegiatan yang dilakukan serta siapa yang boleh menghuni/melakukan kegiatan. Organisasi ruang merupakan manifestasi sifat tertutup, berhati-hati dan curiga masyarakatnya. Bentuk rumah Osing berkaitan erat dengan struktur sosial masyarakat yang cenderung egaliter. Nama bagian-bagian rumah dan susunannya merupakan pengungkapan pesan, makna dan kehendak sebagai ekspresi rasa dan karsa pemiliknya. Makna tersebut tidak terkandung dalam bentuk itu sendiri, melainkan dalam diri manusia yang menginginkan bentuk tersebut mencerminkan sifat laten dan asosiasional, sekaligus menggambarkan apresiasi cipta dan karya. Kata kunci: dualitas dan centralitas; Tikel Balung, Baresan dan Cerocogan.

  20. First molecular identification of the transgene red fluorescent protein (RFP) in transgenic ornamental zebrafish (Danio rerio) introduced in Peru

    OpenAIRE

    Carlos Scotto; Fernando Serna

    2013-01-01

    In this paper the transgenic fluorescent red, orange and pink zebra fish (Danio rerio), found in local aquariums in Peru, were identified using the PCR technique to amplify the transgene RFP sea anemone belonging to Discosoma spp. The gene expression of the red fluorescent protein (RFP) transgene was found to determine different gradients-of-bioluminescence (shades in color) in each GMO fish analyzed. We performed sequence analysis of the two variants of the RFP along with six variants of the...

  1. PENGARUH WORKSHOP DAN SERTIFIKASI AKUNTANSI TERHADAP PEMAHAMAN DASAR AKUNTANSI DAN PRAKTEK ( STUDI KASUS DI AMIK BSI SUKABUMI

    Directory of Open Access Journals (Sweden)

    Yuri Rahayu

    2016-03-01

      Abstrak - Mata kuliah dasar akuntansi dan praktek merupakan mata kuliah unggulan bagi jurusan Komputer Akuntansi dan harus menjadi pondasi yang kuat untuk bisa mengerti dan memahaminya dengan baik dan benar. Tapi pada kenyataannya hanya 30  % saja dari jumlah mahasiswa yang bisa memahami dan mengerti akan mata kuliah tersebut. Untuk bisa mengatasi masalah tersebut di perlukan metode pembelajaran yang lebih mendalam terhadap suatu topik tertentu dengan melakukan  pertukaran informasi dan interaksi antar peserta dengan pembahasan yang bersifat tutorial dan cenderung teknis atau yang di sebut dengan istilah Workshop dan untuk mengetahui sampai sejauh mana pemahaman terhadap  hasil workshop tersebut  perlu di laksanakan Sertifikasi. Penelitian ini untuk mengetahui sampai sejauh mana pengaruhnya Workshop dan Sertifikasi terhadap pemahaman dasar akuntansi. Metode penelitian yang digunakan adalah metode penelitian kuantitatif dan untuk menguji hipotesis dipilih teknik pengumpulan data dengan cara survey dengan  teknik analisisnya menggunakan metode Metode Deskriptif analisis yaitu suatu metode penelitian yang mengambil sample dari suatu populasi dan menggunakan kuesioner sebagai alat pengumpul datanya. Populasi dalam penelitian ini adalah jumlah peserta workshop dan sertifikasi akuntansi  jurusan  Komputer Akuntansi   AMIK BSI  yang berjumlah 242 orang. Pemilihan dan penetapan jumlah sample berdasarkan teknik Sampling purposive sebanyak 48 orang (studi kasus peserta di AMIK BSI Sukabumi. Kesimpulan dari penelitian ini adalah  pelaksanaan workshop dan sertifikasi akuntansi berpengaruh signifikan terhadap pemahaman dasar akuntansi dan praktek. Hal ini menunjukkan pengaruh yang positif dan mempunyai arti bahwa apabila mahasiswa sering mengikuti workshop dan sertifikasi akuntansi maka akan diikuti kenaikan  yang sangat kuat terhadap pemahaman dasar akuntansi dan praktek.   Kata kunci : pemahamana akuntansi, sertifikasi, workshop

  2. MUTU ORGANOLEPTIK DAN KIMIAWI TERASI UDANG REBON DENGAN KADAR GARAM BERBEDA DAN LAMA FERMENTASI

    Directory of Open Access Journals (Sweden)

    apri dwi anggo

    2014-07-01

    Full Text Available Terasi merupakan produk fermentasi udang dengan penambahan garam. Fermentasi dengan garam menyebabkan perombakan protein menjadi asam amino misalnya asam glutamat sebagai penghasil cita rasa khas terasi. Kadar garam dan lama fermentasi merupakan faktor penting pada proses pembuatan terasi. Penelitian ini bertujuan untuk menganalisis pengaruh perbedaan konsentrasi garam dan lama fermentasi terhadap kualitas terasi rebon terutama kandungan asam glutamat. Bahan baku yang digunakan pada penelitian ini adalah rebon (Acetes sp. kering tawar dengan panjang ±2 cm per ekor. Perlakuan garam dengan konsentrasi 2%, 8,5%, 15% dan lama fermentasi (8 hari dan 32 hari. Metode penelitian menggunakan metode experimental field dengan Rancangan Acak Kelompok (RAK pola split plot in time 3 kali ulangan. Parameter yang diuji meliputi organoleptik, pH, kadar protein, asam glutamat, kadar air dan profil asam amino. Parameter kadar protein, asam glutamat dan profil asam amino pada fermentasi hari ke 32 yang diamati hanya perlakuan konsentrasi kadar garam terbaik pada hari ke 8. Nilai organoleptik terasi berkisar antara 7,65-8,32 artinya produk tersebut dapat diterima konsumen. Nilai pH Nilai pH terasi rebon berkisar antara 7,09 sampai 7,89. Konsentrasi garam 2% pada terasi rebon menghasilkan kadar protein dan asam glutamat tertinggi yaitu 47,14%+0,20 (dry base. Asam amino yang dominan pada terasi rebon adalah asam glutamat dan asam aspartat. Lama fermentasi menyebabkan penurunan asam glutamat terasi rebon.Kata kunci: asam glutamat, fermentasi, garam, rebon

  3. Perancangan dan Implementasi Algoritma DES untuk Mikroprosesor Enkripsi dan Dekripsi pada FPGA

    Directory of Open Access Journals (Sweden)

    Imaduddin Amrullah Muslim

    2015-04-01

    Full Text Available Seiring dengan semakin luasnya penerapan teknologi komputasi di sekitar kita, menjadikan informasi menjadi sangat mudah dan cepat untuk disebarkan. Kita dapat mengakses informasi dan data-data yang kita butuhkan dengan mudah. Namun permasalahan yang kita hadapi saat ini kerhasiaan informasi menjadi sangat riskan. Oleh karena itu Sistem keamanan merupakan hal penting yang perlu diperhatikan dalam mengembangkan suatu sistem komputer hal ini lah yang menjadikan enkripsi dan dekripsi data menjadi hal yang penting. Modul rancangan IP Core ini dirancang menggunakan aplikasi Xilinx ISE Design Suite 12.4. Kemudian rancangan IP Core ini diimplementasikan pada papan Xilinx FPGA Spartan-3E XC3S500E-4FG320C dari keluarga Xilinx FPGA Spartan-3E dengan 500K sistem gerbang. Verifikasi fungsional dari IP Core yang dirancang menggunakan testbench dan simulasi diagram pewaktuan menggunakan aplikasi Xilinx ISE Simulator. Tugas akhir ini ditujukan untuk mengembangkan IP Core yang mampu menjalankan fungsi enkripsi DES (Data Encryption Standard dan ditulis menggunakan bahasa Verilog. Implementasi algoritma enkripsi dan dekripsi algoritma DES telah berhasil dilakukan. Hasil analisis menunjukkan sistem telah dapat melakukan enkripsi dan dekripsi data sesuai dengan spesifikasi algoritma DES. Sebaiknya penelitian ini dikembangkan kembali dengan menguji sistem untuk melakukan transmisi data berupa file ataupun teks. Selain itu juga perlu meningkatkan unjuk kerja sistem dengan optimasi sumber daya dan kecepatan waktu proses pada perancangan rekonstruksi kode verilog.

  4. Pengaruh Variasi Waktu Ultrasonikasi dan Waktu Tahan Hydrothermal terhadap Struktur dan Konduktivitas Listrik Material Graphene

    Directory of Open Access Journals (Sweden)

    Muhammad Junaidi

    2014-03-01

    Full Text Available Graphene merupakan satu lapis atom karbon yang mengalami hibridisasi sp2 membentuk struktur heksagonal 2D. Graphene memiliki potensi besar untuk aplikasi nanoelectronic materials. Penelitian ini bertujuan menganalisis waktu ultrasonikasi dan hidrothermal terbaik untuk mensintesis Graphene. Graphene disintesis dengan metode reduksi Grafit Oksida (GO. Grafit dioksidasi membentuk GO dengan modifikasi metode Hummer. GO diultrasonikasi dengan variasi waktu 60, 90 dan 120 menit. Kemudian proses reduksi GO dilakukan secara kimia dengan penambahan serbuk Zn dan teknik hidrothermal 200 oC dengan variasi waktu tahan 12, 18 dan 24 jam. Analisis morfologi dan struktur graphene dilakukan dengan pengujian SEM dan XRD. Pengujian Iodine Number untuk mengetahui kemampuan graphene menyerap Iodin. Pengujian FTIR untuk mengetahui gugus fungsi yang terbentuk  pada GO dan Graphene. Pengujian Four Point Probe Test (FPP untuk mengukur konduktivitas listrik Graphene. Hasil analsis menunjukkan bahwa graphene hasil ultrasonikasi 120 menit dan Holding 12 jam memiliki struktur Single Layer Graphene (SLG dengan konduktivitas listrik terbaik 0.0105 S/m

  5. Gereja dan Paradigma Pembaharuan Ditinjau dari Perspektif Teologi Agama dan Masyarakat

    Directory of Open Access Journals (Sweden)

    Yunny Jones Akal

    2005-04-01

    Full Text Available Gereja atau umat Allah diharapkan selalu berada dalam perubahan positif, agar tegar dan siap terusmelaksanakan tanggung jawab vertical dan horizontal. Prinsip yang perlu adalahgereja memberi kontribusi bagi masyarakat. Peluang-peluang seperti halnyamengembangkan pelayanan berdasarkan panggilan Allah, bermotif kasih, danberorientasikan perbuatan baik menjadi kekuatan lahirnya pengakuan“Allahmu adalah Allahku, Kristusmu adalah Kristusku”

  6. PROFIL ASAM LEMAK DAN KESTABILAN PRODUK FORMULASI MINYAK IKAN DAN HABBATUSSAUDA

    Directory of Open Access Journals (Sweden)

    Sugeng Heri Suseno

    2014-06-01

    Full Text Available Minyak ikan dan minyak habbatussauda saat ini banyak digunakan untuk meningkatkan kecerdasandan mencegah berbagai penyakit. Tujuan penelitian ini adalah menentukan karakteristik dan kestabilanproduk kombinasi minyak ikan dan habbatussauda. Formulasi kombinasi minyak ikan dan habbatussaudayaitu sebesar 1:1, 3:1, 5:1, dan 7:1. Analisis profi l asam lemak menunjukkan bahwa minyak ikan didominasioleh EPA dan DHA, sedangkan komponen utama minyak habbatussauda adalah asam oleat dan asamlinoleat. Minyak habbatussauda memiliki aktivitas antioksidan sebesar 551,17 mM AEAC, sedangkanminyak ikan sebesar 61,15 mM AEAC. Formulasi terbaik berdasarkan uji FFA, bilangan peroksida, dananisidin adalah formulasi 1:1 dengan nilai masing-masing 1,68%, 26,67 meq/kg dan 13,65 meq/kg. Hasilterbaik berdasarkan uji kestabilan adalah produk kombinasi 3:1 dengan nilai FFA terendah hingga harike-9 sebesar 5,94%, sedangkan untuk uji organoleptik adalah formulasi 7:1, paling disukai panelis karenamemiliki warna yang paling cerah.Kata kunci: anisidin, kestabilan, organoleptik, peroksida

  7. Pengaruh Rasio Biji terhadap Pelarut dan Waktu Ekstraksi terhadap Yield dan Kualitas Minyak Biji Alpukat

    Directory of Open Access Journals (Sweden)

    Novia Mehra Erfiza

    2016-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh rasio biji alpukat terhadap pelarut dan waktu ekstraksi pada proses ekstraksi minyak biji alpukat menggunakan pelarut n-heksan. Penelitian menggunakan rancangan acak kelompok dengan pola faktorial terdiri atas 2 faktor yang masing-masing terdiri dari 3 taraf dengan 3 kali ulangan. Faktor pertama adalah rasio biji alpukat terhadap pelarut yaitu 1:5, 1:10 dan 1:15 (g/mL, sedangkan faktor kedua adalah waktu ekstraksi yaitu 120, 150, dan 180 menit. Hasil sidik ragam menunjukkan bahwa perlakuan rasio bahan terhadap pelarut tidak berpengaruh nyata (P>0,05 terhadap yield dan bilangan asam. Sementara itu, perlakuan waktu ekstraksi berpengaruh nyata (P≤0,05 terhadap yield minyak biji alpukat, dan berpengaruh tidak nyata (P>0,05 terhadap bilangan asam. Yield tertinggi (2,23% diperoleh dari sampel minyak pada perlakuan rasio bubuk biji alpukat terhadap pelarut 1:5 dan waktu ekstraksi 120 menit dengan aktivitas antioksidan 62,87%, serta nilai viskositas dan densitas masing-masing yaitu 13,5 cP dan 0,96 g/mL. Asam lemak oleat merupakan komponen terbesar dalam minyak biji alpukat.

  8. KEEFEKTIFAN MODEL PEMBELAJARAN ISK DAN DI DITINJAU DARI MOTIVASI, SIKAP, DAN KEMAMPUAN KOMUNIKASI MATEMATIS

    Directory of Open Access Journals (Sweden)

    Djuwita Amin Mahmud

    2014-11-01

    Full Text Available Penelitian ini bertujuan untuk mendeskripsikan keefektifan dan perbedaan keefektifan model pembelajaran interaktif setting kooperatif dengan direct instruction ditinjau dari motivasi belajar, sikap, dan kemampuan komunikasi matematis. Populasi penelitian ini adalah siswa kelas X SMA Negeri 3 Ternate yang terdiri dari 5 kelas. Dari populasi yang ada diambil secara acak dua kelas yaitu kelas X-1 dan X-3 sebagai sampel penelitian. Kelas X-1 diberi treatment dengan model pembelajaran interaktif setting kooperatif, sedangkan kelas X-3 dengan model direct instruction. Data penelitian dianalisis dengan statistik uji one sampel t-test, uji MANOVA dengan T2 Hotelling’s pada taraf signifikan 0,05 dan uji univariat untuk menentukan model mana yang lebih efektif. Hasil penelitian menunjukkan bahwa: (1 pembelajaran matematika dengan model pembelajaran interaktif setting kooperatif efektif ditinjau dari motivasi belajar, sikap, dan komunikasi matematis sedangkan pada model pembelajaran direct instruction efektif ditinjau dari motivasi belajar dan sikap belajar siswa tetapi tidak efektif ditinjau dari komunikasi matematis; (2 terdapat perbedaan keefektifan antara model pembelajaran interaktif setting kooperatif dengan direct instruction. Model pembelajaran interaktif setting kooperatif lebih efektif daripada model direct instruction ditinjau dari motivasi belajar, sikap, dan kemampuan komunikasi matematis siswa kelas X SMA Negeri 3 Ternate. Kata Kunci: Model pembelajaran interaktif setting kooperatif, direct instruction, motivasi belajar, sikap, dan kemampuan komunikasi matematis.

  9. Profil Bibir dan Posisi Insisivus Perawatan Kasus Borderline Klas I dengan Pencabutan dan Tanpa Pencabutan

    Directory of Open Access Journals (Sweden)

    Yenni Hanimastuti

    2013-12-01

    Full Text Available Penentuan rencana perawatan ortodontik dengan pencabutan atau tanpa pencabutan masih menjadi perdebatan, terutama pada kasus borderline.Perawatan ortodontik dengan atau tanpa pencabutan dapat mempengaruhi profil wajah.Perubahan pada penampilan wajah terjadi akibat adanya perubahan posisi gigi anterior yang dapat mempengaruhi perubahan profil jaringan lunak wajah terutama pada daerah bibir. Penelitian ini bertujuan mengetahui perbandingan perubahan profil bibir dan posisi gigi insisivus pada kasus borderline klas I antara perawatan dengan pencabutan 4 premolar kedua dan tanpa pencabutan. Penelitian dilakukan pada 28 sefalogram lateral kasus borderline klas I  yang dirawat dengan teknik straight wire, terdiri dari 2 kelompok (13 kasus dengan pencabutan dan 15 kasus tanpa pencabutan. Masing-masing sefalogram dilakukan pengukuran profil bibir,yaitu jarak bibir atas dan bawah terhadapVertical Reference Plane (VRP dan sudut interlabial; serta posisi gigi insisivus, yaitu jarak gigi insisivus atas dan bawah terhadap Vertical Reference Plane (VRP, sebelum dan sesudah perawatan. Hasil penelitian menunjukkan pada tahap awal perawatan kedua kelompok memiliki karakteristik profil bibir dan posisi gigi insisivus yang sama (p>0,05. Terdapat perbedaan bermakna (p0,05. There are significant differences (p<0.05 on lips position, interlabial angle, and incisors position changes between the extraction and non- extractiion cases after orthodontic treatment. From this study, it can be concluded that lips profile and incisors position in class I borderline cases treated with the extraction of second bicuspid are more retruded than that of non-extraction cases

  10. KANDUNGAN ASAM LEMAK DAN KARAKTERISTIK FISIKO-KIMIA MINYAK IKAN LELE DAN MINYAK IKAN LELE TERFERMENTASI (FATTY ACID CONTENTS AND PHYSICO-CHEMICAL CHARACTERISTICS OF CATFISH OIL AND FERMENTED CATFISH OIL

    Directory of Open Access Journals (Sweden)

    Iskari Ngadiarti

    2013-06-01

    Full Text Available ABSTRACT Catfish oil and fermented catfish oil have not been developed and commercially produced as catfish derived products. Various processing of catfish oil will change both the composition and physico-chemical characteristics. The objective of this study was to identify the composition of fatty acids and physico-chemical characteristics of both catfish oil (MIL and fermented catfish oil (MILT. Results showed that fatty acid composition of catfish oil was MUFA (36.12%> PUFA> (32.43%> SFA (31.45%, while the composition of fermented catfish oil was MUFA (42.96%> SFA (42.32%> PUFA (15.39%. The fermentation process with lactic acid bacteria increased the content of stearic acid but decreased the content of linoleic acid and linolenic acid. Physical and chemical characteristics of MIL and MILT were almost similar, they indicated oxidation process, based on melting point, viscosity, and thiobarbituric acid values. Therefore, in the production of catfish oil or fermented cat fish oil, it is necessary to add spices and/or nutrient as antioxidants sources. Keywords: catfish oil, PUFA, MUFA, SFA and CLA  ABSTRAK Minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT sebagai produk turunan dari ikan lele masih belum banyak dikembangkan dan diproduksi secara komersial. Berbagai proses pengolahan minyak dapat mengubah komposisi dan karakteristik fisiko-kimia minyak ikan lele. Tujuan dari penelitian ini adalah untuk mengidentifikasi komposisi asam lemak dan karakteristik fisiko-kimia dari minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT. Komposisi asam lemak pada MIL secara berurutan adalah MUFA (36,12% > PUFA (32,43% > SFA (31,45%, sedangkan setelah difermentasi menjadi MILT terjadi perubahan yaitu MUFA (42,96% > SFA (42,32% > PUFA (15,39%. Jenis asam lemak jenuh yang mengalami peningkatan  pada MILT adalah asam lemak stearat, sedangkan asam lemak tidak jenuh ganda yang mengalami penurunan adalah asam linoleat dan linolenat yang

  11. [Investigation of 1q21 amplification in patients with multiple myeloma using I-FISH and cIg-FISH].

    Science.gov (United States)

    Yang, Rui-fang; Li, Chun-ming; Qiu, Hai-rong; Lu, Hua; Wu, Han-xin; Xu, Jia-ren; Zhang, Peng; Li, Jian-yong; Chen, Li-juan

    2011-12-01

    To investigate the prevlance of 1q21 amplification in patients with multiple myeloma (MM) and its correlation with the progression and prognosis of the disease. 1q21 amplification was detected in 48 patients with MM using cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization analysis (cIg-FISH) and interphase fluorescence in situ hybridization (I-FISH) analysis combined with CD138 immunomagnetic cell sorting (MACS). 1q21 amplification (≥ 3 red signals) was detected in 26/48(54.2%) cases by cIg-FISH and 31/48 (64.6%) cases by I-FISH combined with CD138 MACS. There was a good consistency between the two methods (P>0.05). The mortality of patients with 1q21 amplification was significantly higher than those without (P0.05). The frequency of 1q21 amplification in MM is high. There was also an association between the amplification and poor prognosis. cIg-FISH is consistent with CD138 MACS combined with I-FISH.

  12. Fluorescence and Spectral Imaging

    Directory of Open Access Journals (Sweden)

    Ralph S. DaCosta

    2007-01-01

    Full Text Available Early identification of dysplasia remains a critical goal for diagnostic endoscopy since early discovery directly improves patient survival because it allows endoscopic or surgical intervention with disease localized without lymph node involvement. Clinical studies have successfully used tissue autofluorescence with conventional white light endoscopy and biopsy for detecting adenomatous colonic polyps, differentiating benign hyperplastic from adenomas with acceptable sensitivity and specificity. In Barrett's esophagus, the detection of dysplasia remains problematic because of background inflammation, whereas in the squamous esophagus, autofluorescence imaging appears to be more dependable. Point fluorescence spectroscopy, although playing a crucial role in the pioneering mechanistic development of fluorescence endoscopic imaging, does not seem to have a current function in endoscopy because of its nontargeted sampling and suboptimal sensitivity and specificity. Other point spectroscopic modalities, such as Raman spectroscopy and elastic light scattering, continue to be evaluated in clinical studies, but still suffer the significant disadvantages of being random and nonimaging. A recent addition to the fluorescence endoscopic imaging arsenal is the use of confocal fluorescence endomicroscopy, which provides real-time optical biopsy for the first time. To improve detection of dysplasia in the gastrointestinal tract, a new and exciting development has been the use of exogenous fluorescence contrast probes that specifically target a variety of disease-related cellular biomarkers using conventional fluorescent dyes and novel potent fluorescent nanocrystals (i.e., quantum dots. This is an area of great promise, but still in its infancy, and preclinical studies are currently under way.

  13. Essential Fish Habitat (EFH) Areas Protected From Fishing

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Designated Essential Fish Habitat (EFH) areas where fishing or the use of fishing gears has been restricted or modified in order to minimize the adverse effects of...

  14. Fish silage as feed ingredient for fish and livestock

    NARCIS (Netherlands)

    Rurangwa, E.; Vuuren, van A.M.; Poelman, M.

    2014-01-01

    The present report analyses through a literature review the potential of fish silage to valorise fish processing by-products into economically relevant protein sources for fish and livestock feed production in East Africa.

  15. Fish Springs NWR mammal, fish, amphibian, and reptile list

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The following is a species list for mammals, fishes, amphibians, and reptiles found on or adjacent to Fish Springs National Wildlife Refuge, as of October, 1996.

  16. PERKEMBANGAN DAN EKSISTENSI MUSIK TARLING CIREBON

    Directory of Open Access Journals (Sweden)

    Salim -

    2015-08-01

    Full Text Available Kemajuan teknologi berpengaruh terhadap perkembangan Tarling namun demikian Tarling masih tetap menunjukan eksistensinya. Nampaknya ada hal yang menarik bagi peneliti untuk selanjutnya mengadakan penelitian tentang perkembangan dan eksistensi Tarling. Sehubungan dengan permasalahan tersebut maka masalah penelitiannya adalah bagaimana perkembangan Tarling, dan Bagaimana eksistensi Tarling. Penelitian Tesis ini menggunakan metoda penelitian kualitatif. Peneliti berusaha mendeskripsikan perkembangan dan eksistensi Tarling. Untuk memahami  perkembangan, dan eksistensi maka penelitian ini  menggunakan teori perkembangan dan eksistensi. Teknik pengumpulan data dilakukan dengan cara observasi, wawancara, dan dokumentasi. Pemeriksaan keabsahan data dilakukan dengan  menggunakan metode tringulasi. Analisis data dilakukan dengan tahapan: Reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukan bahwa berdasarkan perkembangannya Tarling mengalami beberapa periodisasi perubahan yaitu periode musik, periode  lagu dan lawak kemudian periode teater. Berkenaan dengan eksistensinya musik Tarlingpun mengalami perubahan bentuk yaitu Tarling kreasi dan Tarling dangdut. Eksistensi musik Tarling terbentuk melalui proses akulturasi. Saran dari hasil penelitian menyatakan bahwa, perubahan yang terjadi dalam  Tarling dimaksudkan sebagai upaya agar Tarling tetap menarik kemudian eksis. Maka bagi Para tokoh masyarakat, seniman Tarling, pemerintah dan masyarakat Cirebon harus bersinergi melakukan berbagai langkah agar Tarling tetap menujukan eksistensinya The progress of technology influence the development Tarling, however Tarling still shows its existence. It seems that there is something interesting for researchers to conduct further research on the development and existence Tarling. In connection with these problems, the research problem is how the development Tarling and How existence Tarling. This thesis research uses qualitative

  17. Mineralization Of PAHs In Coal-Tar Impacted Aquifer Sediments And Associated Microbial Community Structure Investigated With FISH

    Science.gov (United States)

    The microbial community structure and mineralization of polycyclic aromatic hydrocarbons (PAHs) in a coal-tar contaminated aquifer were investigated spatially using fluorescence in situ hybridization (FISH) and in laboratory-scale incubations of the aquifer sediments. DAPI-detect...

  18. PERAN TEKNOLOGI KOMUNIKASI DALAM INTERAKSI AYAH DAN ANAK STUDI KASUS: INTERAKSI AYAH DAN ANAK MELALUI SMARTPHONE

    Directory of Open Access Journals (Sweden)

    Detania Savitri

    2016-02-01

    Fungsi ayah dalam keluarga adalah sebagai pencari nafkah untuk memenuhi kebutuhan keluarga, sementara ibu berfungsi dalam mendidik anak. Ayah yang bekerja penuh waktu akan berkurang interaksinya dengan anak. Disisi lain, teknologi komunikasi diciptakan untuk mempermudah interaksi antar individu. Penelitian ini ingin mengetahui apakah smartphone dapat meningkatkan intensitas kedekatan interaksi antara ayah dan anak. Selain itu, penelitian ini juga ingin mengetahui apakah ayah dapat memberikan sosialisasi kepada anak melalui smartphone dan mengontrol keseharian anak. Artikel ini menggunakan perspektif fungsionalis. Pendekatan kualitatif digunakan oleh penulis dengan mewawancarai tiga ayah dan tiga anak berusia 20 tahun ke atas yang sama-sama menggunakan smartphone di kota Depok. Temuan yang diperoleh penulis yaitu smartphone dapat merubah interaksi ayah dengan anak, dan ayah dapat memberikan kontrol sosial kepada anak dalam interaksi melalui smartphone. Dalam interaksi ayah dan anak, smartphone juga dapat digunakan ayah untuk mensosialisasikan nilai-nilai kepada anak.

  19. HASIL ANALISIS VITAMIN A DAN β-KAROTEN BAHAN MAKANAN SUMBER VITAMIN A DAN KAROTEN DENGAN METODE HPLC

    OpenAIRE

    Yuniar Rosmalina; Dewi Permaesih

    2012-01-01

    Telah dilakukan analisis vitamin A DAN β-Karoten terhadap beberapa jenis pangan dengan menggunakan metode HPLC. Analisis dilakukan terhadap 9 jenis serealia dan umbi-umbian, 3 jenis kacang-kacangan, 40 jenis sayuran, 11 jenis daging dan hasilnya, 7 jenis telur dan hasilnya, 8 jenis ikan, 12 jenis buah-buahaan, dan 7 jenis kelompok lain-lain. Hasilnya menunjukkan sumber β-Karoten yang tinggi pada kelompok serealia dan umbi-umbian adalah umbi jalar, pada kelompok sayuran adalah daun katuk, daun...

  20. Pengembangan Sistem Informasi Penjualan dan Pembelian pada Toko PC Tablet

    Directory of Open Access Journals (Sweden)

    Nur Setyo Permatasasi Putri W

    2014-06-01

    Full Text Available Sistem informasi penjualan dan pembelian yang dimiliki oleh perusahaan masih mempunyai beberapa kekurangan yang menyebabkan terjadinya kesalahan data pada laporan yang dihasilkan. Dan juga meskipun perusahaan telah memiliki sistem informasi namun perusahaan masih sering melakukan proses transaksi dan pencatatan transaksi secara manual, sehingga kesalahan data tidak dapat terhindarkan lagi. Oleh karena itu, perusahaan membutuhkan sebuah sistem informasi penjualan dan pembelian yang memadai, untuk dapat menampilkan data lebih akurat dan lebih mudah dipahami dalam penggunaannya. Pengembangan sistem informasi penjualan dan pembelian diawali dengan analisis kebutuhan sistem. Hal ini ditujukan untuk mendapatkan spesifikasi sistem yang sesuai. Kemudian dari data yang ada, dilakukan perancangan sistem informasi penjualan dan pembelian yang disesuaikan dengan kebutuhan perusahaan. Dalam tahapan perancangan, penggambaran proses bisnis sistem informasi dijelaskan dengan menggunakan DFD (Data Flow Diagram dan untuk merepresentasikan data dalam sistem secara logis dilakukan dengan menggunakan ERD (Entity Relationship Diagram. Hasil dari perancangan kemudian diimplementasikan dengan menggunakan bahasa pemrograman PHP dan menggunakan sistem basis data MySQL. Perangkat yang digunakan adalah perangkat lunak Adobe Dreamweaver CS6 dan XAMPP. Hasil pengujian sistem informasi penjualan dan pembelian menunjukkan bahwa semua fitur yang terdapat dalam sistem informasi dapat bekerja dengan baik seperti mengelola data transaksi, mengelola data admin, mengelola data barang dan mengelola data lainnya yang berhubungan dengan kegiatan pokok dalam transaksi ini. Pada sistem informasi penjualan dan pembelian juga disertakan fungsi untuk menampilkan laporan dari proses transaksi yang dilakukan oleh pengguna.

  1. Hantaran Sinyal Leptin dan Obesitas: Hubungannya dengan Penyakit Kardiovaskuler

    Directory of Open Access Journals (Sweden)

    David Limanan

    2013-11-01

    Full Text Available Diperkirakan saat ini jumlah orang dengan obesitas melebihi 250 juta orang, yaitu 7% dari populasi orang dewasa di dunia. Mortalitas obesitas erat hubungannya dengan sindrom metabolik yang merupakan kelainan metabolik meliputi obesitas, resistensi insulin, gangguan toleransi glukosa, abnormalitas trigliserida dan hemostasis, disfungsi endotel dan hipertensi. Leptin dihasilkan adiposit dan merupakan anggota dari adipositokin; berperan dalam hantaran sinyal hormon jaringan adiposa. Kelainan leptin maupun reseptornyadapat menyebabkan seseorang mengalami obesitas, metabolik sindrom, diabetes dan penyakit kardiovaskuler. Kompleks leptin-reseptor mengaktifkan sistem transduksi sinyal, yang paling dominan adalah jalur janus kinase-signal transducer and activator of transcription-3 (JAK-STAT3, kemudian phospatidyl inositol 3- kinase (PI3K, mitogen-activated protein kinase (MAPK, 5’adenosine monophosphate-activated protein kinase (AMPK, dan mammalian target of rapamycin (mTOR. Jalur leptin-associated PI3K dengan ERK cascade berperan penting dalam proliferasi kardiomiosit dan melindungi jantung dari ischemia reperfusion injury. ERK1/2 mengaktifkan target gen seperti c-fos dan egr-1 yang berperan dalam proliferasi dan diferensiasi. Nuclear factor κB diduga sebagai target jalur p38 dan JNK MAPK. Faktor transkripsi inu berperan pentingdalam mengatur transkripsi sitokin proinflamasi seperti tumor necrosis factor (TNF-α dan interleukin (IL-1β. Leptin dapat meningkatkan pembentukan reactive oxygen species (ROS sel endotel pembuluh darah dan menstimulasi sekresi TNF-α dan IL-6 yang merupakan promotor hipertensi dan aterosklerosis.Kata Kunci: obesitas, leptin, sistem kardiovaskuler 

  2. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M [Santa Fe, NM; Waldo, Geoffrey S [Santa Fe, NM; Kiss, Csaba [Los Alamos, NM

    2012-05-01

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  3. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M.; Waldo, Geoffrey S.; Kiss, Csaba

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  4. ISOLASI BAKTERI HETEROTROF DI SITU CIBUNTU, JAWA BARAT DAN KARAKTERISASI RESISTENSI ASAM DAN LOGAM

    Directory of Open Access Journals (Sweden)

    Kesi Kurnia

    2016-12-01

    Full Text Available AbstrakBakteri heterotrofik sangat berperan penting dalam sistem perairan karena kemampuan aktivitas metabolismenya. Bakteri tersebut berinteraksi dengan logam dan mineral dalam lingkungan alam dan sintetis, mengubah keadaan fisik dan kimianya sehingga mempengaruhi aktivitas, pertumbuhan dan kelangsungan hidup bakteri. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri resisten logam timbal (Pb dan asam di Situ Cibintu, Cibinong, Jawa Barat. Sampel air diambil dari 3 titik di Situ Cibuntu dan dianalisis distribusi bakteri heterotrofiknya. Isolat bakteri dikultur di media Triptone Glucose Yeast (TGY. Selanjutnya dilakukan resistensi terhadap logam timbal (Pb dan asam. Hasil penelitian menunjukkan bahwa jumlah bakteri heterotrof di Situ Cibuntu rata-rata 3,5x103 cfu m/L. Mayoritas (73% isolat bakteri resisten terhadap logam timbal sebanyak 47% dan resisten terhadap asam (pH 4. Bakteri yang resisten didominasi oleh Gram negatif. Penelitian ini memberikan pengetahuan mengenai kemampuan adaptasi bakteri heterotrof pada lingkungan logam dan asam.Abstract Heterotrophic bacteria play an important role in aquatic systems because of their ability of metabolic activities. They interact with metals and minerals in natural and synthetic environments, altering their physical and chemical state, with metals and minerals also able to affect activity, microbial growth and survival. The purpose of study is to isolate and characterize the lead (Pb and acid resistant of heterotrophic bacteria strains isolated from pond Cibuntu, Cibinong, West Java. Water samples were collected from three points around pond Cibuntu and analyzed the distribution of heterotrophic bacteria. The bacterial isolates were screened on Triptone Glucose Yeast (TGY agar plates. Lead (Pb metal and acidic tolerance were tested. In the present study, the population density of heterotrophic bacteria were recorded 3.5x103 cfu m/L. The majority (73% of bacteria isolates were

  5. KEPEMIMPINAN, SISTEM DAN STRUKTUR ORGANISASI, LINGKUNGAN FISIK, DAN KEEFEKTIFAN ORGANISASI SEKOLAH

    Directory of Open Access Journals (Sweden)

    Connie Chairunnisa

    2013-05-01

    Full Text Available Abstract: The Effects of Leadership, System and Structure, and Physical Environment on the Ef­fectiveness of School Organization. The study aims at obtaining information of the effects of leadership, system and structure, and physical environment on the effectiveness of organization. This causal study was conducted in one hundred schools in Jakarta. Survey method with causal approach and  path analysis was employed. The population consisted of288 teachers in junior high schools in DKI Province of Jakarta. The sample of 100 teachers was selected by applying simple random sampling technique. The results show di­rect effects of (1 system and structure on physical environment,  (2  leadership  on the effectiveness, (3 system and structure on physical environment as well as the effectiveness of organization, (4 physical en­vironment on the effectiveness of organization. In addition, system and structure show indirect effect on the effectiveness of organization through the physical environment. The research concludes that any change or variation in organizational effectiveness can be influenced by leadership, system and structure, and physical environment. Abstrak: Kepemimpinan, Sistem dan Struktur Organisasi, Lingkungan Fisik, dan Keefektifan Orga­nisasi. Penelitian ini bertujuan untuk mendeskripsikan pengaruh kepemimpinan, sistem dan struktur organi­sasi, dan lingkungan fisik terhadap keefektifan organisasi. Penelitian diselenggarakan di seratus sekolah di Jakarta, dengan menggunakan metode survei melalui pendekatan kausal dan analisis jalur. Sampel sebanyak 100 guru SMP Negeri Provinsi DKI Jakarta diambil dengan teknik penyampelan acak sederhana. Hasil penelitian menunjukkan bahwa terdapat pengaruh langsung dari (1 sistem dan struktur organisasi terhadap lingkungan fisik; (2 kepemimpinan kepala sekolah terhadap keefektifan organisasi; (3 sistem dan struktur terhadap lingkungan fisik juga terhadap keefektifan organisasi; (4 lingkungan fisik

  6. Variasi Temporal dan Stabilitas Fisik dan Kimia Senyawa Bioaktif Karotenoid Rumput Laut Coklat Turbinaria decurrens

    Directory of Open Access Journals (Sweden)

    Rini Susilowati

    2014-06-01

    Full Text Available Pada pengembangan senyawa bioaktif karotenoid dari rumput laut coklat Turbinaria decurrens sebagai produk nutrasetikal atau pangan fungsional, pengetahuan mengenai variasi temporal dan stabilitas fisik dan kimia senyawa tersebut merupakan hal yang penting. Penelitian ini bertujuan untuk menjelaskan variasi temporal dan stabilitas fisik dan kimia senyawa karotenoid dari T. decurrens. Bahan baku T. decurrens diambil dari Pantai Binuangeun, Banten. Kuantifikasi kadar karotenoid pada sampel dilakukan terhadap ekstrak kasar etanol dari sampel dengan menggunakan teknik spektrofotometri UV-Vis. Variasi temporal kadar karotenoid dilakukan dengan pengambilan sampel pada musim penghujan (Februari, musim peralihan (April dan Oktober, serta musim kemarau (Juni dan Agustus, pada tahun 2012. Analisis variabel air (DO, pH, suhu, temperatur, salinitas, fosfat, dan nitrogen anorganik terlarut dari titik pengambilan sampel juga dilakukan, untuk mengetahui faktor utama yang mempengaruhi produksi senyawa ini di alam. Analisis stabilitas senyawa dilakukan dengan perlakuan penambahan asam, basa, oksidator, dan suhu. Hasil pengujian menunjukan bahwa kadar karotenoid temporal dari alga ini berkisar 0,002–0,063 mg/g (bobot segar. Analisis korelasi terhadap variabel air menunjukkan bahwa kadar karotenoid dipengaruhi faktor musim, tidak tergantung pada variabel air lokal. Terkait dengan probabilitas fungsinya dalam fotosintesis, kadar tertinggi senyawa ini terdapat pada musim penghujan. Karotenoid dapat meluruh sebesar 84% pada perlakuan asam, 15% pada perlakukan basa, 28% pada perlakukan oksidasi, dan 15% pada suhu tinggi (70 oC. Dengan demikian, pada pengembangannya sebagai produk pangan fungsional atau nutrasetikal, diperlukan teknik mikroenkapsulasi untuk menjaga stabilitas fisik dan kimia senyawa karotenoid T. decurrens.

  7. Alaskan sport fishing waters

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — As a guide to newcomers and visitors, fishery biologists have compiled a list of some of the well-known fishing waters in Alaska. The list is merely a starting point...

  8. Ammonia toxicity in fish.

    Science.gov (United States)

    Randall, D J; Tsui, T K N

    2002-01-01

    Ammonia is present in the aquatic environment due to agricultural run-off and decomposition of biological waste. Ammonia is toxic to all vertebrates causing convulsions, coma and death, probably because elevated NH4+ displaces K+ and depolarizes neurons, causing activation of NMDA type glutamate receptor, which leads to an influx of excessive Ca2+ and subsequent cell death in the central nervous system. Present ammonia criteria for aquatic systems are based on toxicity tests carried out on, starved, resting, non-stressed fish. This is doubly inappropriate. During exhaustive exercise and stress, fish increase ammonia production and are more sensitive to external ammonia. Present criteria do not protect swimming fish. Fish have strategies to protect them from the ammonia pulse following feeding, and this also protects them from increases in external ammonia, as a result starved fish are more sensitive to external ammonia than fed fish. There are a number of fish species that can tolerate high environmental ammonia. Glutamine formation is an important ammonia detoxification strategy in the brain of fish, especially after feeding. Detoxification of ammonia to urea has also been observed in elasmobranches and some teleosts. Reduction in the rate of proteolysis and the rate of amino acid catabolism, which results in a decrease in ammonia production, may be another strategy to reduce ammonia toxicity. The weather loach volatilizes NH3, and the mudskipper, P. schlosseri, utilizes yet another unique strategy, it actively pumps NH4+ out of the body.

  9. PARASITES OF FISH

    Science.gov (United States)

    The intent of this chapter is to describe the parasites of importance to fishes maintained and used in laboratory settings. In contrast to the frist edition, the focus will be only on those parasites that pose a serious threat to or are common in fishes held in these confined en...

  10. Hawaiian Fish Distributors Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is proprietary sales data from one Kona-based fish dealer for about one year back in the late 1980s. Fishing was generally around Kona. This is Dealer Data and...

  11. Virus diseases of fish

    Science.gov (United States)

    Watson, Stanley W.

    1954-01-01

    Viruses are probably the cause of a wide spectrum of fish diseases. Although relatively few virus diseases of fish are known today, some of the diseases of unknown etiology, as well as some diseases presently accepted as due to bacteria, protozoa, fungi or nutritional deficiencies, possibly will be recognized eventually as virus diseases.

  12. Enzymes in Fermented Fish.

    Science.gov (United States)

    Giyatmi; Irianto, H E

    Fermented fish products are very popular particularly in Southeast Asian countries. These products have unique characteristics, especially in terms of aroma, flavor, and texture developing during fermentation process. Proteolytic enzymes have a main role in hydrolyzing protein into simpler compounds. Fermentation process of fish relies both on naturally occurring enzymes (in the muscle or the intestinal tract) as well as bacteria. Fermented fish products processed using the whole fish show a different characteristic compared to those prepared from headed and gutted fish. Endogenous enzymes like trypsin, chymotrypsin, elastase, and aminopeptidase are the most involved in the fermentation process. Muscle tissue enzymes like cathepsins, peptidases, transaminases, amidases, amino acid decarboxylases, glutamic dehydrogenases, and related enzymes may also play a role in fish fermentation. Due to the decreased bacterial number during fermentation, contribution of microbial enzymes to proteolysis may be expected prior to salting of fish. Commercial enzymes are supplemented during processing for specific purposes, such as quality improvement and process acceleration. In the case of fish sauce, efforts to accelerate fermentation process and to improve product quality have been studied by addition of enzymes such as papain, bromelain, trypsin, pepsin, and chymotrypsin. © 2017 Elsevier Inc. All rights reserved.

  13. Folkbiology of Freshwater Fish

    Science.gov (United States)

    Medin, Douglas L.; Ross, Norbert O.; Atran, Scott; Cox, Douglas; Coley, John; Proffitt, Julia B.; Blok, Sergey

    2006-01-01

    Cross-cultural comparisons of categorization often confound cultural factors with expertise. This paper reports four experiments on the conceptual behavior of Native American and majority-culture fish experts. The two groups live in the same general area and engage in essentially the same set of fishing-related behaviors. Nonetheless, cultural…

  14. PENINGKATAN MINAT DAN KEPUTUSAN BERPARTISIPASI AKSEPTOR KB

    Directory of Open Access Journals (Sweden)

    Ken Sudarti

    2011-09-01

    Full Text Available Tujuan dari studi ini adalah untuk menemukan dampak dari kualitas layanan dan konseling yang disediakan oleh PLKB dan persepsi pengguna kontrasepsi pada budaya lingkungan yang berkontribusi terhadap ketertarikan dan keputusan para pengguna alat kotrasepsi. Populasi penelitian ini adalah 98 wanita yang melakukan program keluarga berencana dalam jangka waktu lama dengan menggunakan sample convenience. Penelitian ini menunjukkan mayoritas pengguna kontrasepsi adalah usia rawan untuk melahirkan, memiliki lebih dari dua anak, dan relatif memiliki latar belakang pendidikan rendah. Kualitas layanan program konseling dan budaya lingkungan dalam program keluarga berencana mempunyai dampak positif terhadap ketertarikan dan keputusan untuk berpartisipasi terhadap program keluarga berencana. Kualitas layanan ditemukan sebagai kontribusi terbesar terhadap peningkatan ketertarikan dan partisipasi dalam program keluarga berencana.The aim of this study was to find out the effect of the service quality and counceling provided by the family planning field workers (PLKB and the perception of the contraception users on the environmental culture that contribute to the interest and decision of the contraception users.The population of this study was the contraception users in Demak District. The traditional statistical model of yamae was applied to recruit the 98 women who were long-term users of birth control within the government-sponsored family planning program using the convenience sampling method.The study showed that the majority of the contraception users were in the age at risk of giving birth , with more than two children and relatively had low education background. The service quality, councelling program and environmental culture had a possitive effect on the interest and the decision of participating in the family planning program . Service quality was found to give the biggest contribution for the improved interest and partisipation in the family

  15. PENINGKATAN MINAT DAN KEPUTUSAN BERPARTISIPASI AKSEPTOR KB

    Directory of Open Access Journals (Sweden)

    Ken Sudarti

    2011-09-01

    Full Text Available Tujuan dari studi ini adalah untuk menemukan dampak dari kualitas layanan dan konseling yang disediakan oleh PLKB dan persepsi pengguna kontrasepsi pada budaya lingkungan yang berkontribusi terhadap ketertarikan dan keputusan para pengguna alat kotrasepsi. Populasi penelitian ini adalah 98 wanita yang melakukan program keluarga berencana dalam jangka waktu lama dengan menggunakan sample convenience. Penelitian ini menunjukkan mayoritas pengguna kontrasepsi adalah usia rawan untuk melahirkan, memiliki lebih dari dua anak, dan relatif memiliki latar belakang pendidikan rendah. Kualitas layanan program konseling dan budaya lingkungan dalam program keluarga berencana mempunyai dampak positif terhadap ketertarikan dan keputusan untuk berpartisipasi terhadap program keluarga berencana. Kualitas layanan ditemukan sebagai kontribusi terbesar terhadap peningkatan ketertarikan dan partisipasi dalam program keluarga berencana.The aim of this study was to find out the effect of the service quality and counceling provided by the family planning field workers (PLKB and the perception of the contraception users on the environmental culture that contribute to the interest and decision of the contraception users.The population of this study was the contraception users in Demak District. The traditional statistical model of yamae was applied to recruit the 98 women who were long-term users of birth control within the government-sponsored family planning program using the convenience sampling method.The study showed that the majority of the contraception users were in the age at risk of giving birth , with more than two children and relatively had low education background. The service quality, councelling program and environmental culture had a possitive effect on the interest and the decision of participating in the family planning program . Service quality was found to give the biggest contribution for the improved interest and partisipation in the family

  16. Biannual Fish Survey, Spring 1993

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The biannual fish survey was initiated in 1989 to monitor population trends of federally endangered fish species at Ash Meadows National Wildlife Refuge. Item 421 of...

  17. Perawatan Kandidiasis Pseuodomembran Akut dan Mukositis Oral pada Penderita Kanker Nasofaring yang Menerima Khemoterapi dan Radioterapi

    Directory of Open Access Journals (Sweden)

    S. Supriatno

    2016-10-01

    Full Text Available Latar belakang: Terapi radiasi merupakan metode primer perawatan pasien kanker leher dan kepala. Perubahan funsional dan kerusakan jaringan oral menyebabkan timbulnya mukositia oral yang diikuti dengan kandidiasis oral. Tujuan: Melaporkan efek samping perawatan khemoterapi dan radioterapi pada pasien kanker nasofaring yang terjadi di rongga mulut berupa kandidiasis pseudomembran akut dan mukositis oral serta penatalaksanaannya. Kasus: Seorang laki-laki, 69 tahun, datang ke Bagian Gigi dan Mulut RSUP Dr. Sardjito, atas rujukan dari instalasi Penyakit Dalam., RSUP Dr. Sardjito, dengan keluhan sakit untuk menelan makanan dan mulutnya banyak bercak-bercak putih. Keluhan dirasakan satu minggu setelah dilakukan khemoterapi ke-3 dan radioterapi ke-9. Pasien didiagnosa kanker nasofaring (NPC dengan klasifikasi T2N3M0. Pemeriksaan klinik menunjukkan adanya lapisan putih pada mukosa lidah, pipi, palatum, dan mukosa bibir. Seluruh mukosa mulut berwarna merah tua dan terdapat anguler cheilitis di kedua sudut bibir. Pasien diklasifikasikan menderita mukositis oral derajat 1. Penatalaksanaan: Menghilangkan jaringan nekrotik dan debris dengan berkumur larutan perhidrol 3% dan pemberian medikasi termasuk tablet nistatin 500.000 IU, betadin kumur, dan larutan perhidrol 3% selama 1 minggu. Saat reevaluasi, pasien sudah dapat menelan dan makan yang sedikit keras tanpa ada rasa sakit lagi. Pemeriksaan klinis didapatkan bercak putih di lidah, palatum, pipi dan bibir sudah tidak ada. Warna mukosa oral telah normal, OHI dan kondisi umum baik dalam 1 minggu pasca perawatan. Kesimpulan: Perawatan kandidiasis dan mukositis oral akibat kemoradioterapi pada pasien kanker nasofaring telah berhasil dan kondisi oral membaik. Pasien dapat mengunyah dan menelan makanan tanpa ada rasa sakit, dan hasil pengobatan yang diberikan pada pasien sesuai dengan harapan operator.   Background: Radiation therapy remains the primary method of treatment for patients with head and neck

  18. PEMBELAJARAN KETERAMPILAN BERBICARA MODEL KOOPERATIF TEKNIK MENCARI PASANGAN DAN TEKNIK KANCING GEMERINCING PADA SISWA INTROVER DAN EKSTROVER DI SMP

    Directory of Open Access Journals (Sweden)

    Sri Sunarsih

    2012-06-01

    Full Text Available Unit Penelitian dan Pengabdian pada Masyarakat merupakan salah satu unit di Universitas Respati Yogyakarta yang bertugas untuk mengelola kegiatan penelitian dan pengabdian kepada masyarakat yang dilakukan oleh dosen. Dalam proses pengolahan data kegiatan kegiatan penelitian dan pengabdian kepada masyarakat masih mengalami beberapa permasalahan yaitu belum adanya basis data yang digunakan untuk pengumpulan data rekam jejak kegiatan penelitian dan pengabdian kepada masyarakat oleh dosen dan sistem pengarsipan dokumen masih berbentuk kertas yang berdampak pada kerusakan dokumen, hilangnya dokumen, efisiensi ruang dan pencarian data. Penelitian yang dilakukan bertujuan menghasilkan luaran sistem informasi yang dapat melakukan proses pengolahan data penelitian dan kegiatan pengabdian kepada masyarakat yang dikelola oleh Unit Penelitian dan Pengabdian pada Masyarakat di Universitas Respati Yogyakarta. Pengolahan data mengacu pada komponen beban kerja dosen dibidang penelitian dan pengabdian kepada masyarakat. Metode pengembangan dilakukan dengan tahapan analisa sistem, perancangan sistem, coding dan implementasi sistem. Hasil pengembangan sistem informasi penelitian dan pengabdian masyarakat yaitu sebuah sistem yang dapat membantu Unit Penelitian dan Pengabdian pada Masyarakat dalam proses pengolahan data rekam jejak kegiatan penelitian dan kegiatan pengabdian kepada masyarakat yang dilakukan dosen.

  19. UJI AKTIVITAS ANTIBAKTERI EKSTRAK RUMPUT LAUT Kappaphycus alvarezii DAN Eucheuma denticullatum TERHADAP BAKTERI Aeromonas hydrophila DAN Vibrio harveyii

    Directory of Open Access Journals (Sweden)

    Dwi Budi Wiyanto

    2010-04-01

    Full Text Available Penelitian tentang Uji Aktivitas Antibakteri Ekstrak Rumput Laut Kappaphycus alvarezii dan Eucheuma denticullatum Terhadap Bakteri Aeromonas hydrophila dan Vibrio harveyii dilakukan, mengingat banyak dijumpai  penyakit pada usaha budidaya ikan dan udang, terutama bakteri Aeromonas hydrophila dan Vibrio harveyii. Penelitian ini bertujuan untuk mengetahui efektifitas senyawa bioaktif rumput laut K. alvarezii dan E. denticullatum yang diekstrak menggunakan pelarut metanol dan etanol sebagai antibakteri terhadap A. hydrophila dan V. harveyii.  Penelitian dilakukan sebanyak dua tahap, yaitu: (1 Uji aktivitas antibakteri ekstrak rumput laut; dan (2 Analisa senyawa bioaktif yang terdapat pada ekstrak rumput laut, dimana masing-masing tahapan dianalisis menggunakan rancangan acak lengkap faktorial. Hasil penelitian menunjukkan, dua jenis ekstrak rumput laut dengan pelarut metanol dan etanol, mempunyai daya antibakteri terhadap A. hydrophila dan V. harveyii. Ekstrak E. denticullatum dengan pelarut metanol memiliki daya hambat lebih luas dibanding ekstrak K. alvarezii dengan pelarut metanol terhadap A. hydrophila (19.43±0,55 mm. Ekstrak E. denticullatum dengan pelarut metanol memiliki daya hambat lebih luas dibanding ekastrak K. alvarezii dengan pelarut metanol terhadap V. harveyii (19.85±0,23 mm. Asam heksadekanoat merupakan senyawa paling dominan dijumpai pada ekstrak rumput laut K. alvarezii, dan E. denticullatum yang diekstrak menggunakan pelarut metanol. Kata Kunci : Aktivitas Antibakteri, Rumput laut K. alvarezii dan E. denticullatum,  Bakteri A.  hydrophila dan V. harveyii. 

  20. PENGARUH METODE PENGERINGAN DAN KONSENTRASI BUMBU SERTA LAMA PERENDAMAN DALAM LARUTAN BUMBU TERHADAP KUALITAS FISIK DAN SENSORI DENDENG BABI

    Directory of Open Access Journals (Sweden)

    Marcus Veerman

    2013-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh metode pengeringan dan konsentrasi bumbu serta lama perendaman terhadap pH dan sensori dendeng daging babi. Irisan daging direndam dalam larutan bumbu dengan konsentrasi 40, 20, 13,3, dan 10%. Lamanya perendaman adalah 1, 3, 5, dan 7 jam, kemudian dilakukan pengeringan dengan sinar matahari dan oven. Parameter yang diamati meliputi pH dan parameter sensori. Data pH dianalisis dengan analisis variansi Rancangan Acak Lengkap pola faktorial 2×4×4 dengan tiga kali ulangan, dan dilanjutkan dengan uji jarak berganda Duncan. Data uji sensori dianalisis dengan analisis non parametrik  menggunakan uji Hedonik menurut Kruskal-Wallis, dilanjutkan  analisis deskriptif kuantitatif (jaring labalaba pada program MS Excel. Metode pengeringan dan konsentrasi bumbu serta lama perendaman, dan interaksi ketiga perlakuan berpengaruh sangat nyata (P<0,01 terhadap pH dendeng daging babi. Berdasarkan diagram jaring laba-laba, uji sensori meliputi parameter warna menunjukkan coklat sampai coklat keemasan, rasa menunjukkan agar suka sampai sangat suka dan daya terima menunjukkan agak suka sampai sangat suka. Metode pengeringan matahari menghasilkan tekstur kasar sampai agak kasar dan keempukan menunjukkan keras sampai agak keras. Metode pengeringan oven menunjukkan parameter warna agak coklat sampai coklat keemasan dan rasa agak suka sampai sangat suka, sedangkan daya terima tidak suka sampai agak suka, tekstur kasar sampai agak kasar dan keempukan keras sampai agak keras. (Kata kunci: Dendeng babi, Kualitas pH, Uji sensori, Metode pengeringan, Konsentrasi bumbu