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Sample records for fluorescence fish dans

  1. Diversity and Ecological Correlates of Red Fluorescence in Marine Fishes

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    Nils Anthes

    2016-11-01

    Full Text Available Marine environments at depths below -10 to -25 m are almost devoid of ambient red sunlight because water quickly attenuates long wavelengths. This stenospectral light environment presents unique opportunities for organisms that can transform ambient blue-green light into red light by fluorescence. Numerous marine fish species display intricate patterns of fluorescence. Because color vision is a key component of fish sensory ecology, several putative visual functions of red fluorescence have been proposed but are difficult to test experimentally. Here, we follow a comparative approach to assess the consistency between the phylogenetic distribution of red fluorescence with its presumed functions. We collected and analyzed the largest data set of red fluorescence in fishes to date, consisting of confirmed cases in 272 primarily diurnal fish species from 49 out of 90 surveyed fish families and 12 out of 21 surveyed fish orders, contrasted to 393 fish species with confirmed absence of red fluorescence. Based on a priori hypotheses on adaptive function, we compare the prevalence of red fluorescence among pre-defined sets of species based on ecological or biological characteristics while controlling for shared ancestry. When comparing between species, we find no evidence that red fluorescence is more prevalent in deep-water species, contrasting with our recent finding that fluorescence brightness increases with depth within species. There is also no evidence for a role in group-driven communication. Phylogenetic patterns are consistent, however, with three other predictions. First, fluorescence with a rather patchy distribution across the body occurred significantly more often among sit-and-wait predators or otherwise sedentary fish than in more mobile species, consistent with background matching for camouflage. Second, small, predatory fishes tended to show red fluorescent irides disproportionally often consistent with a proposed function in prey

  2. Red fluorescence in reef fish: A novel signalling mechanism?

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    Siebeck Ulrike E

    2008-09-01

    Full Text Available Abstract Background At depths below 10 m, reefs are dominated by blue-green light because seawater selectively absorbs the longer, 'red' wavelengths beyond 600 nm from the downwelling sunlight. Consequently, the visual pigments of many reef fish are matched to shorter wavelengths, which are transmitted better by water. Combining the typically poor long-wavelength sensitivity of fish eyes with the presumed lack of ambient red light, red light is currently considered irrelevant for reef fish. However, previous studies ignore the fact that several marine organisms, including deep sea fish, produce their own red luminescence and are capable of seeing it. Results We here report that at least 32 reef fishes from 16 genera and 5 families show pronounced red fluorescence under natural, daytime conditions at depths where downwelling red light is virtually absent. Fluorescence was confirmed by extensive spectrometry in the laboratory. In most cases peak emission was around 600 nm and fluorescence was associated with guanine crystals, which thus far were known for their light reflecting properties only. Our data indicate that red fluorescence may function in a context of intraspecific communication. Fluorescence patterns were typically associated with the eyes or the head, varying substantially even between species of the same genus. Moreover red fluorescence was particularly strong in fins that are involved in intraspecific signalling. Finally, microspectrometry in one fluorescent goby, Eviota pellucida, showed a long-wave sensitivity that overlapped with its own red fluorescence, indicating that this species is capable of seeing its own fluorescence. Conclusion We show that red fluorescence is widespread among marine fishes. Many features indicate that it is used as a private communication mechanism in small, benthic, pair- or group-living fishes. Many of these species show quite cryptic colouration in other parts of the visible spectrum. High inter

  3. 21 CFR 866.4700 - Automated fluorescence in situ hybridization (FISH) enumeration systems.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated fluorescence in situ hybridization (FISH... Laboratory Equipment and Reagents § 866.4700 Automated fluorescence in situ hybridization (FISH) enumeration... Hybridization (FISH) Enumeration Systems.” See § 866.1(e) for the availability of this guidance document. [70 FR...

  4. Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish.

    Science.gov (United States)

    Hardison, D Ransom; Holland, William C; McCall, Jennifer R; Bourdelais, Andrea J; Baden, Daniel G; Darius, H Taiana; Chinain, Mireille; Tester, Patricia A; Shea, Damian; Quintana, Harold A Flores; Morris, James A; Litaker, R Wayne

    2016-01-01

    Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R)). However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA(R) in certain labs. A fluorescence based receptor binding assay (RBA(F)) was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY®-PbTx-2) for voltage-gated sodium channel receptors at site 5 instead of a radiolabeled brevetoxin. Responses were linear in fish tissues spiked from 0.1 to 1.0 ppb with Pacific ciguatoxin-3C (P-CTX-3C) with a detection limit of 0.075 ppb. Carribean ciguatoxins were confirmed in Caribbean fish by LC-MS/MS analysis of the regional biomarker (C-CTX-1). Fish (N = 61) of six different species were screened using the RBA(F). Results for corresponding samples analyzed using the neuroblastoma cell-based assay (CBA-N2a) correlated well (R2 = 0.71) with those of the RBA(F), given the low levels of CTX present in positive fish. Data analyses also showed the resulting toxicity levels of P-CTX-3C equivalents determined by CBA-N2a were consistently lower than the RBA(F) affinities expressed as % binding equivalents, indicating that a given amount of toxin bound to the site 5 receptors translates into corresponding lower cytotoxicity. Consequently, the RBA(F), which takes approximately two hours to perform, provides a generous estimate relative to the widely used CBA-N2a which requires 2.5 days to complete. Other RBA(F) advantages include the long-term (> 5 years) stability of the BODIPY®-PbTx-2 and having similar results as the commonly used RBA(R). The RBA(F) is cost-effective, allows high sample

  5. Fluorescent Receptor Binding Assay for Detecting Ciguatoxins in Fish.

    Directory of Open Access Journals (Sweden)

    D Ransom Hardison

    Full Text Available Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs. One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA(R. However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA(R in certain labs. A fluorescence based receptor binding assay (RBA(F was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY®-PbTx-2 for voltage-gated sodium channel receptors at site 5 instead of a radiolabeled brevetoxin. Responses were linear in fish tissues spiked from 0.1 to 1.0 ppb with Pacific ciguatoxin-3C (P-CTX-3C with a detection limit of 0.075 ppb. Carribean ciguatoxins were confirmed in Caribbean fish by LC-MS/MS analysis of the regional biomarker (C-CTX-1. Fish (N = 61 of six different species were screened using the RBA(F. Results for corresponding samples analyzed using the neuroblastoma cell-based assay (CBA-N2a correlated well (R2 = 0.71 with those of the RBA(F, given the low levels of CTX present in positive fish. Data analyses also showed the resulting toxicity levels of P-CTX-3C equivalents determined by CBA-N2a were consistently lower than the RBA(F affinities expressed as % binding equivalents, indicating that a given amount of toxin bound to the site 5 receptors translates into corresponding lower cytotoxicity. Consequently, the RBA(F, which takes approximately two hours to perform, provides a generous estimate relative to the widely used CBA-N2a which requires 2.5 days to complete. Other RBA(F advantages include the long-term (> 5 years stability of the BODIPY®-PbTx-2 and having similar results as the commonly used RBA(R. The RBA(F is cost-effective, allows high sample

  6. A simple protocol for attenuating the auto-fluorescence of cyanobacteria for optimized fluorescence in situ hybridization (FISH) imaging.

    Science.gov (United States)

    Zeller, Perrine; Ploux, Olivier; Méjean, Annick

    2016-03-01

    Cyanobacteria contain pigments, which generate auto-fluorescence that interferes with fluorescence in situ hybridization (FISH) imaging of cyanobacteria. We describe simple chemical treatments using CuSO4 or H2O2 that significantly reduce the auto-fluorescence of Microcystis strains. These protocols were successfully applied in FISH experiments using 16S rRNA specific probes and filamentous cyanobacteria. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Red fluorescence increases with depth in reef fishes, supporting a visual function, not UV protection

    Science.gov (United States)

    Meadows, Melissa G.; Anthes, Nils; Dangelmayer, Sandra; Alwany, Magdy A.; Gerlach, Tobias; Schulte, Gregor; Sprenger, Dennis; Theobald, Jennifer; Michiels, Nico K.

    2014-01-01

    Why do some marine fishes exhibit striking patterns of natural red fluorescence? In this study, we contrast two non-exclusive hypotheses: (i) that UV absorption by fluorescent pigments offers significant photoprotection in shallow water, where UV irradiance is strongest; and (ii) that red fluorescence enhances visual contrast at depths below −10 m, where most light in the ‘red’ 600–700 nm range has been absorbed. Whereas the photoprotection hypothesis predicts fluorescence to be stronger near the surface and weaker in deeper water, the visual contrast hypothesis predicts the opposite. We used fluorometry to measure red fluorescence brightness in vivo in individuals belonging to eight common small reef fish species with conspicuously red fluorescent eyes. Fluorescence was significantly brighter in specimens from the −20 m sites than in those from −5 m sites in six out of eight species. No difference was found in the remaining two. Our results support the visual contrast hypothesis. We discuss the possible roles fluorescence may play in fish visual ecology and highlight the possibility that fluorescent light emission from the eyes in particular may be used to detect cryptic prey. PMID:25030989

  8. MOTIVASI, SIKAP, DAN INTENSI PENGGUNA MEDIA SOSIAL PADA KAMPANYE STOP ILLEGAL FISHING

    Directory of Open Access Journals (Sweden)

    La Moriansyah

    2016-11-01

    Full Text Available Social media is commonly used to promote social campaigns, one of which is Stop Illegal Fishing campaign. To achieve success in this campaign, some conditions have to be fulfilled i.e. positive attitudes of the social media users toward the campaigns and high intentions to provide recommendations (word of mouth to others. This study was conducted to analyze types of motivations affecting attitudes of users towards social campaigns (stop illegal fishing in social media. In addition, it also analyzed the influence of attitudes on users’ intentions in performing WOM. The method utilized to meet the objectives was Partial Least Square. The results showed that users’ motivation in using social media and towards the campaign messages are perceived to have significantly a positive effect on the attitude towards social campaigns in social media. Besides, the increase in the intention to perform WOM is positively influenced by the attitudes of social media users. There are differences identified in the motivation that affect the attitudes of social media users towards campaign of the two groups i.e. the social media users who have never seen the Stop Illegal Fishing campaign (151 samples and those who have seen the campaign in social media.    Keywords:  motivation, attitude, word of mouth, user experience, uses and gratification theory, digital campaigns, social campaigns, stop illegal fishingABSTRAKMedia sosial sering digunakan untuk mempromosikan kampanye sosial, contohnya adalah  kampanye Stop Illegal Fishing. Untuk meraih kesuksesan pada kampanye ini, beberapa hal yang harus diraih adalah sikap positif pengguna media sosial terhadap kampanye dan intensi yang tinggi untuk memberikan rekomendasi (word of mouth kepada orang lain. Penelitian ini dilakukan untuk menganalisis motivasi pengguna apa saja yang memengaruhi sikap pengguna media sosial terhadap kampanye sosial (stop illegal fishing di media sosial. Selain itu menganalisis sikap

  9. Fluorescence In Situ Hybridization (FISH Signal Analysis Using Automated Generated Projection Images

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    Xingwei Wang

    2012-01-01

    Full Text Available Fluorescence in situ hybridization (FISH tests provide promising molecular imaging biomarkers to more accurately and reliably detect and diagnose cancers and genetic disorders. Since current manual FISH signal analysis is low-efficient and inconsistent, which limits its clinical utility, developing automated FISH image scanning systems and computer-aided detection (CAD schemes has been attracting research interests. To acquire high-resolution FISH images in a multi-spectral scanning mode, a huge amount of image data with the stack of the multiple three-dimensional (3-D image slices is generated from a single specimen. Automated preprocessing these scanned images to eliminate the non-useful and redundant data is important to make the automated FISH tests acceptable in clinical applications. In this study, a dual-detector fluorescence image scanning system was applied to scan four specimen slides with FISH-probed chromosome X. A CAD scheme was developed to detect analyzable interphase cells and map the multiple imaging slices recorded FISH-probed signals into the 2-D projection images. CAD scheme was then applied to each projection image to detect analyzable interphase cells using an adaptive multiple-threshold algorithm, identify FISH-probed signals using a top-hat transform, and compute the ratios between the normal and abnormal cells. To assess CAD performance, the FISH-probed signals were also independently visually detected by an observer. The Kappa coefficients for agreement between CAD and observer ranged from 0.69 to 1.0 in detecting/counting FISH signal spots in four testing samples. The study demonstrated the feasibility of automated FISH signal analysis that applying a CAD scheme to the automated generated 2-D projection images.

  10. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

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    Niels Tommerup

    2010-11-01

    Full Text Available Fluorescence in situ Hybridization (FISH is a major cytogenetic technique for clinical genetic diagnosis of both inherited and acquired chromosomal abnormalities. Although FISH techniques have evolved and are often used together with other cytogenetic methods like CGH, PRINS and PNA-FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3–5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies.

  11. Fish with red fluorescent eyes forage more efficiently under dim, blue-green light conditions.

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    Harant, Ulrike Katharina; Michiels, Nicolaas Karel

    2017-04-20

    Natural red fluorescence is particularly conspicuous in the eyes of some small, benthic, predatory fishes. Fluorescence also increases in relative efficiency with increasing depth, which has generated speculation about its possible function as a "light organ" to detect cryptic organisms under bluish light. Here we investigate whether foraging success is improved under ambient conditions that make red fluorescence stand out more, using the triplefin Tripterygion delaisi as a model system. We repeatedly presented 10 copepods to individual fish (n = 40) kept under a narrow blue-green spectrum and compared their performance with that under a broad spectrum with the same overall brightness. The experiment was repeated for two levels of brightness, a shaded one representing 0.4% of the light present at the surface and a heavily shaded one with about 0.01% of the surface brightness. Fish were 7% more successful at catching copepods under the narrow, fluorescence-friendly spectrum than under the broad spectrum. However, this effect was significant under the heavily shaded light treatment only. This outcome corroborates previous predictions that fluorescence may be an adaptation to blue-green, heavily shaded environments, which coincides with the opportunistic biology of this species that lives in the transition zone between exposed and heavily shaded microhabitats.

  12. Diagnostic and Prognostic Utility of Fluorescence In situ Hybridization (FISH) Analysis in Acute Myeloid Leukemia.

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    Gonzales, Patrick R; Mikhail, Fady M

    2017-12-01

    Acute myeloid leukemia (AML) is a hematologic neoplasia consisting of incompletely differentiated hematopoietic cells of the myeloid lineage that proliferate in the bone marrow, blood, and/or other tissues. Clinical implementation of fluorescence in situ hybridization (FISH) in cytogenetic laboratories allows for high-resolution analysis of recurrent structural chromosomal rearrangements specific to AML, especially in AML with normal karyotypes, which comprises approximately 33-50% of AML-positive specimens. Here, we review the use of several FISH probe strategies in the diagnosis of AML. We also review the standards and guidelines currently in place for use by clinical cytogenetic laboratories in the evaluation of AML. Updated standards and guidelines from the WHO, ACMG, and NCCN have further defined clinically significant, recurring cytogenetic anomalies in AML that are detectable by FISH. FISH continues to be a powerful technique in the diagnosis of AML, with higher resolution than conventional cytogenetic analysis, rapid turnaround time, and a considerable diagnostic and prognostic utility.

  13. Automation of ALK gene rearrangement testing with fluorescence in situ hybridization (FISH): a feasibility study.

    Science.gov (United States)

    Zwaenepoel, Karen; Merkle, Dennis; Cabillic, Florian; Berg, Erica; Belaud-Rotureau, Marc-Antoine; Grazioli, Vittorio; Herelle, Olga; Hummel, Michael; Le Calve, Michele; Lenze, Dido; Mende, Stefanie; Pauwels, Patrick; Quilichini, Benoit; Repetti, Elena

    2015-02-01

    In the past several years we have observed a significant increase in our understanding of molecular mechanisms that drive lung cancer. Specifically in the non-small cell lung cancer sub-types, ALK gene rearrangements represent a sub-group of tumors that are targetable by the tyrosine kinase inhibitor Crizotinib, resulting in significant reductions in tumor burden. Phase II and III clinical trials were performed using an ALK break-apart FISH probe kit, making FISH the gold standard for identifying ALK rearrangements in patients. FISH is often considered a labor and cost intensive molecular technique, and in this study we aimed to demonstrate feasibility for automation of ALK FISH testing, to improve laboratory workflow and ease of testing. This involved automation of the pre-treatment steps of the ALK assay using various protocols on the VP 2000 instrument, and facilitating automated scanning of the fluorescent FISH specimens for simplified enumeration on various backend scanning and analysis systems. The results indicated that ALK FISH can be automated. Significantly, both the Ikoniscope and BioView system of automated FISH scanning and analysis systems provided a robust analysis algorithm to define ALK rearrangements. In addition, the BioView system facilitated consultation of difficult cases via the internet. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Heavy metals analysis in fishes by the X-ray fluorescence method

    International Nuclear Information System (INIS)

    Perez Novara, Ana Ma.

    1986-04-01

    Among the sources of contamination in human beings we find ingestion of heavy metals. As it is common practice to pour industrial wastes in waters where fishes feed, some toxic elements present in water may pass to human beings through ingestion. It is therefore important to determine the concentrations of heavy metals present in fishes, mainly in those living in waters close to industrial zones or villages. Concentrations of heavy metals in tissue of fishes amount to ppm, hence making necessary the use of very sensitive analytical techniques which do not require a too complex preparation of the sample in order to avoid the loss or contamination of interesting elements of analysis while handling them, thus falsifying the results. The X-Ray Fluorescence method covers these requirements and is not destructive nor multi-elemental. The development of the technique of element analysis in fishes by X-Ray Fluorescence comprised several aspects. from sampling and storage to quantification, specially stressing the preparation of samples. The work was carried out with a Si-Li detector/monitor for solid state and associated electronic equipment. Cd-109 and Pu-238 sources were used to produce excitation, detection limits near 1 ppm were obtained in the majority of elements the technique attained for the analysis of this kind of samples fulfills the celerity, precision, accuracy, and sensitivity requirements. (author)

  15. Fluorescence in situ hybridization (CARD-FISH) of microorganisms in hydrocarbon contaminated aquifer sediment samples.

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    Tischer, Karolin; Zeder, Michael; Klug, Rebecca; Pernthaler, Jakob; Schattenhofer, Martha; Harms, Hauke; Wendeberg, Annelie

    2012-12-01

    Groundwater ecosystems are the most important sources of drinking water worldwide but they are threatened by contamination and overexploitation. Petroleum spills account for the most common source of contamination and the high carbon load results in anoxia and steep geochemical gradients. Microbes play a major role in the transformation of petroleum hydrocarbons into less toxic substances. To investigate microbial populations at the single cell level, fluorescence in situ hybridization (FISH) is now a well-established technique. Recently, however, catalyzed reporter deposition (CARD)-FISH has been introduced for the detection of microbes from oligotrophic environments. Nevertheless, petroleum contaminated aquifers present a worst case scenario for FISH techniques due to the combination of high background fluorescence of hydrocarbons and the presence of small microbial cells caused by the low turnover rates characteristic of groundwater ecosystems. It is therefore not surprising that studies of microorganisms from such sites are mostly based on cultivation techniques, fingerprinting, and amplicon sequencing. However, to reveal the population dynamics and interspecies relationships of the key participants of contaminant degradation, FISH is an indispensable tool. In this study, a protocol for FISH was developed in combination with cell quantification using an automated counting microscope. The protocol includes the separation and purification of microbial cells from sediment particles, cell permeabilization and, finally, CARD-FISH in a microwave oven. As a proof of principle, the distribution of Archaea and Bacteria was shown in 60 sediment samples taken across the contaminant plume of an aquifer (Leuna, Germany), which has been heavily contaminated with several ten-thousand tonnes of petroleum hydrocarbons since World War II. Copyright © 2012 Elsevier GmbH. All rights reserved.

  16. Two decades of water vapor measurements with the FISH fluorescence hygrometer: a review

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    J. Meyer

    2015-07-01

    Full Text Available For almost two decades, the airborne Fast In-situ Stratospheric Hygrometer (FISH has stood for accurate and precise measurements of total water mixing ratios (WMR, gas phase + evaporated ice in the upper troposphere and lower stratosphere (UT/LS. Here, we present a comprehensive review of the measurement technique (Lyman-α photofragment fluorescence, calibration procedure, accuracy and reliability of FISH. Crucial for FISH measurement quality is the regular calibration to a water vapor reference, namely the commercial frost-point hygrometer DP30. In the frame of this work this frost-point hygrometer is compared to German and British traceable metrological water standards and its accuracy is found to be 2–4 %. Overall, in the range from 4 to 1000 ppmv, the total accuracy of FISH was found to be 6–8 %, as stated in previous publications. For lower mixing ratios down to 1 ppmv, the uncertainty reaches a lower limit of 0.3 ppmv. For specific, non-atmospheric conditions, as set in experiments at the AIDA chamber – namely mixing ratios below 10 and above 100 ppmv in combination with high- and low-pressure conditions – the need to apply a modified FISH calibration evaluation has been identified. The new evaluation improves the agreement of FISH with other hygrometers to ± 10 % accuracy in the respective mixing ratio ranges. Furthermore, a quality check procedure for high total water measurements in cirrus clouds at high pressures (400–500 hPa is introduced. The performance of FISH in the field is assessed by reviewing intercomparisons of FISH water vapor data with other in situ and remote sensing hygrometers over the last two decades. We find that the agreement of FISH with the other hygrometers has improved over that time span from overall up to ± 30 % or more to about ± 5–20 % @ 10 ppmv. As presented here, the robust and continuous calibration and operation procedures of the FISH instrument over the last two decades establish the

  17. Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science

    OpenAIRE

    Ratan, Zubair Ahmed; Zaman, Sojib Bin; Mehta, Varshil; Haidere, Mohammad Faisal; Runa, Nusrat Jahan; Akter, Nasrin

    2017-01-01

    Fluorescence in situ hybridization (FISH) is a macromolecule recognition technique, which is considered as a new advent in the field of cytology.?Initially, it was developed as a physical mapping tool to delineate genes within chromosomes. The accuracy and versatility of FISH were subsequently capitalized upon in biological and medical research. This visually appealing technique provides an intermediate degree of resolution between DNA analysis and chromosomal investigations. FISH consists of...

  18. Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

    DEFF Research Database (Denmark)

    Ronander, Elena; Bengtsson, Dominique C; Joergensen, Louise

    2012-01-01

    Adhesion of Plasmodium falciparum infected erythrocytes (IE) to human endothelial receptors during malaria infections is mediated by expression of PfEMP1 protein variants encoded by the var genes. The haploid P. falciparum genome harbors approximately 60 different var genes of which only one has...... been believed to be transcribed per cell at a time during the blood stage of the infection. How such mutually exclusive regulation of var gene transcription is achieved is unclear, as is the identification of individual var genes or sub-groups of var genes associated with different receptors...... fluorescent in situ hybridization (FISH) analysis of var gene transcription by the parasite in individual nuclei of P. falciparum IE(1). Here, we present a detailed protocol for carrying out the RNA-FISH methodology for analysis of var gene transcription in single-nuclei of P. falciparum infected human...

  19. Diversitas Dan Hilangnya Jenis-jenis Ikan Disungai Ciliwung Dan Sungai Cisadane [Study of Fish Diversity and the Lost of Fish Species of River Ciliwung and R. Cisadane

    OpenAIRE

    Hadiaty, Renny Kurnia

    2011-01-01

    The fish research in Indonesian waters has been begun since 16 century ago. Most of the research collected fish around Batavia.Many new species was described and the type specimens deposited at the museums in Europe or America.The study of fish diversity and the lost of fish species was conducted at River Ciliwung and R. Cisadane in 2009. The aim of this study is to describe the recent fish diversity in both river drainages, then make a comparison with the number of species recorded based on ...

  20. Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells.

    Science.gov (United States)

    Oulhen, Marianne; Pailler, Emma; Faugeroux, Vincent; Farace, Françoise

    2017-01-01

    Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.

  1. Detection of airborne Legionella while showering using liquid impingement and fluorescent in situ hybridization (FISH).

    Science.gov (United States)

    Deloge-Abarkan, Magali; Ha, Thi-Lan; Robine, Enric; Zmirou-Navier, Denis; Mathieu, Laurence

    2007-01-01

    Aerosols of water contaminated with Legionella bacteria constitute the only mode of exposure for humans. However, the prevention strategy against this pathogenic bacteria risk is managed through the survey of water contamination. No relationship linked the Legionella bacteria water concentration and their airborne abundance. Therefore, new approaches in the field of the metrological aspects of Legionella bioaerosols are required. This study was aimed at testing the main principles for bioaerosol collection (solid impaction, liquid impingement and filtration) and the in situ hybridization (FISH) method, both in laboratory and field assays, with the intention of applying such methodologies for airborne Legionella bacteria detection while showering. An aerosolization chamber was developed to generate controlled and reproducible L. pneumophila aerosols. This tool allowed the identification of the liquid impingement method as the most appropriate one for collecting airborne Legionella bacteria. The culturable fraction of airborne L. pneumophila recovered with the liquid impingement principle was 4 and 700 times higher compared to the impaction and filtration techniques, respectively. Moreover, the concentrations of airborne L. pneumophila in the impinger fluid were on average 7.0 x 10(5) FISH-cells m(-3) air with the fluorescent in situ hybridization (FISH) method versus 9.0 x 10(4) CFU m(-3) air with the culture method. These results, recorded under well-controlled conditions, were confirmed during the field experiments performed on aerosols generated by hot water showers in health institutions. This new approach may provide a more accurate characterization of aerobiocontamination by Legionella bacteria.

  2. Determination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Lecrenier, M C; Ledoux, Q; Berben, G; Fumière, O; Saegerman, C; Baeten, V; Veys, P

    2014-07-17

    Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application.

  3. Screening for dioxin contamination in fish oil by PARAFAC and N-PLSR analysis of fluorescence landscapes

    DEFF Research Database (Denmark)

    Kjær Pedersen, D.; Munck, L.; Balling Engelsen, S.

    2002-01-01

    A preliminary investigation of fish oils demonstrates that fluorescence excitation-emission landscapes evaluated by 3-way chemometric methods may be a candidate for an inexpensive screening method to indicate the level of contamination by dioxins and PCB’s which are normally analysed with expensive...

  4. Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science

    Science.gov (United States)

    Ratan, Zubair Ahmed; Zaman, Sojib Bin; Haidere, Mohammad Faisal; Runa, Nusrat Jahan; Akter, Nasrin

    2017-01-01

    Fluorescence in situ hybridization (FISH) is a macromolecule recognition technique, which is considered as a new advent in the field of cytology. Initially, it was developed as a physical mapping tool to delineate genes within chromosomes. The accuracy and versatility of FISH were subsequently capitalized upon in biological and medical research. This visually appealing technique provides an intermediate degree of resolution between DNA analysis and chromosomal investigations. FISH consists of a hybridizing DNA probe, which can be labeled directly or indirectly. In the case of direct labeling, fluorescent nucleotides are used, while indirect labeling is incorporated with reporter molecules that are subsequently detected by fluorescent antibodies or other affinity molecules. FISH is applied to detect genetic abnormalities that include different characteristic gene fusions or the presence of an abnormal number of chromosomes in a cell or loss of a chromosomal region or a whole chromosome. It is also applied in different research applications, such as gene mapping or the identification of novel oncogenes. This article reviews the concept of FISH, its application, and its advantages in medical science.  PMID:28690958

  5. Fluorescent in situ hybridization (FISH as a diagnostic tool for Williams-Beuren syndrome

    Directory of Open Access Journals (Sweden)

    Deise Helena de Souza

    2007-01-01

    Full Text Available Fluorescent in situ hybridization (FISH with commercial probes covering the elastin gene (ELN was used to determine the frequency of the 7q11.23 deletion in 18 children clinically diagnosed with Williams-Beuren syndrome (WBS. A de novo deletion was detected in 15 of the children (83%. Diagnostic investigation for WBS started late in childhood (median = 5.8 years. All the children showed facial features typical of the syndrome, mental retardation and developmental delay. Over-friendliness was observed in the majority of cases. Clinodactyly of the 5th finger (n = 13, cardiovascular disease (n = 9, loquacity (n = 9, low birthweight (n = 8, and failure to thrive (n = 9 were observed only in those children with the deletion. Respiratory problems (n = 9, though not previously reported in the literature, was a common finding in the group studied. Our results confirmed that FISH is useful in identifying 7q11.23 deletions in cases of WBS. Clinical manifestations were more evident in the deletion-positive children.

  6. Detection of trace tetracycline in fish via synchronous fluorescence quenching with carbon quantum dots coated with molecularly imprinted silica

    Science.gov (United States)

    Yang, Ji; Lin, Zheng-Zhong; Nur, A.-Zha; Lu, Yan; Wu, Ming-Hui; Zeng, Jun; Chen, Xiao-Mei; Huang, Zhi-Yong

    2018-02-01

    A novel fluorescence-based sensor combining synchronous fluorescence spectroscopy (SFS) with molecularly imprinted polymers (MIPs) was fabricated with reverse microemulsion method. Tetracycline (TC), (3-aminopropyl) triethoxysilane (APTES), tetraethyl orthosilicate (TEOS) and carbon quantum dots (CDs) were used as template, functional monomer, cross-linker and signal sources respectively in the probe preparation. A synchronous fluorescence emission (λem) at 355 nm was observed for the prepared MIP-coated CDs (MIP@CDs) particles when the wavelength interval (Δλ) was set as 70 nm, and the synchronous fluorescence intensity could be rapidly and efficiently quenched by TC based on inner filter effect (IFE). The quenching efficiencies of synchronous fluorescence intensity was linearly fitted with tetracycline (TC) concentrations ranging from 0.1 to 50 μmol L- 1 with a detection limit (DL) of 9 nmol L- 1 (3σ, n = 9). The MIP@CDs was used as a probe to detect TC in fish samples with the recoveries ranging from 98.4% to 103.1% and the relative standard deviation less than 6.0%. The results illustrated that the as-prepared MIP@CDs could be applied to the detection of trace TC in fish samples with rapidity, high sensitivity and accuracy.

  7. Evaluation of HER-2/neu status in breast cancer specimens using immunohistochemistry (IHC) & fluorescence in-situ hybridization (FISH) assay.

    Science.gov (United States)

    Goud, Kalal Iravathy; Dayakar, Seetha; Vijayalaxmi, Kolanupaka; Babu, Saidam Jangu; Reddy, P Vijay Anand

    2012-03-01

    Fluorescence in situ hybridization (FISH) is increasingly being recognized as the most accurate and predictive test for HER 2/neu gene amplification and response to therapy in breast cancer. In the present study we investigated HER-2/neu gene amplification by FISH in breast carcinoma tissue specimens and compared the results with that of immunohistochemical (IHC) analysis. A total of 90 breast carcinoma tissue samples were used for immunohistochemical (IHC) and FISH analysis. IHC was performed by using mouse monoclonal antibody to the intracellular domain of HER-2/neu protein. Each slide was scored in a blinded fashion by two pathologists according to the manufacturer's recommended criteria. FISH analysis was performed on paraffin embedded breast tumour tissue sections. The polysomy for centromere 17 (Spec green signal) was read as green signals less than 4 as moderate polysomy, and more than 4 as highly polysomy. Thirty of the 90 patients had negative results by IHC and FISH. Of the 28 patients with the score of 2+ by IHC, 20 were FISH positive for HER-2/neu gene amplification, three were FISH negative and five patients showed equivocal (1.8-2.2) results by FISH. These five cases were retested for IHC and FISH on different paraffin embedded tissue blocks, and all five were found positive for HER-2/neu gene amplification. Twenty five patients with the score of 3+ by IHC were FISH positive for HER-2/neu gene amplification (>2.2). Seven cases with the score of 3+ by IHC were FISH negative for HER-2/neu gene amplification (>2.2), and showed polysomy of chromosome number 17 high polysomy > 4. Our results indicated that HER-2/neu status by FISH should be performed in all cases of breast tumour with a 2+ score by IHC. Cases demonstrating a 3+ score by IHC may be subjected to FISH to rule out polysomy of chromosome 17 which could be falsely interpreted as HER-2/neu overexpression by IHC analysis. There is also a need for establishing a clinically validated cut-off value

  8. LED-FISH: Fluorescence microscopy based on light emitting diodes for the molecular analysis of Her-2/neu oncogene amplification

    Directory of Open Access Journals (Sweden)

    Vollmer Ekkehard

    2008-12-01

    Full Text Available Abstract Light emitting diodes (LED, which are available as small monochromatic light sources with characteristic features such as maximum illumination power combined with minimum energy consumption and extremely long lifespan have already proved as a highly potential low-cost alternative for specific diagnostic applications in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the most reliable evaluation of Her-2/neu (c-erbB2 gene amplification, which has been established in the last few years for routine diagnosis in clinical pathology as determinant towards Herceptin-based treatment of patients with breast cancer, is based on fluorescence in situ hybridization (FISH and corresponding high priced fluorescence equipment. In order to test the possibility to utilize the advantages of low-cost LED technology on FISH analysis of c-erbB2 gene expression for routine diagnostic purposes, the applicability of a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER* LED Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination with a conventional 100W mercury vapor lamp. Both microscopes were fitted with the same Quicam FAST CCD digital camera to unequivocally compare the quality of the captured images. C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. The Her2/neu gene signals (green were identifiable in the tumor cells in all cases and images of equal quality were captured under almost identical conditions by 480 nm (blue LED module equipped standard Axiostar microscope as compared to conventional fluorescence microscopy. In this first attempt, these monochromatic LED elements proved in principle to be suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own

  9. [Molecular beacon based PNA-FISH method combined with fluorescence scanning for rapid detection of Listeria monocytogenes].

    Science.gov (United States)

    Wu, Shan; Zhang, Xiaofeng; Shuai, Jiangbing; Li, Ke; Yu, Huizhen; Jin, Chenchen

    2016-07-04

    To simplify the PNA-FISH (Peptide nucleic acid-fluorescence in situ hybridization) test, molecular beacon based PNA probe combined with fluorescence scanning detection technology was applied to replace the original microscope observation to detect Listeria monocytogenes The 5′ end and 3′ end of the L. monocytogenes specific PNA probes were labeled with the fluorescent group and the quenching group respectively, to form a molecular beacon based PNA probe. When PNA probe used for fluorescence scanning and N1 treatment as the control, the false positive rate was 11.4%, and the false negative rate was 0; when N2 treatment as the control, the false positive rate decreased to 4.3%, but the false negative rate rose to 18.6%. When beacon based PNA probe used for fluorescence scanning, taken N1 treatment as blank control, the false positive rate was 8.6%, and the false negative rate was 1.4%; taken N2 treatment as blank control, the false positive rate was 5.7%, and the false negative rate was 1.4%. Compared with PNA probe, molecular beacon based PNA probe can effectively reduce false positives and false negatives. The success rates of hybridization of the two PNA probes were 83.3% and 95.2% respectively; and the rates of the two beacon based PNA probes were 91.7% and 90.5% respectively, which indicated that labeling the both ends of the PNA probe dose not decrease the hybridization rate with the target bacteria. The combination of liquid phase PNA-FISH and fluorescence scanning method, can significantly improve the detection efficiency.

  10. mathFISH, a Web Tool That Uses Thermodynamics-Based Mathematical Models for In Silico Evaluation of Oligonucleotide Probes for Fluorescence In Situ Hybridization▿ †

    OpenAIRE

    Yilmaz, L. Safak; Parnerkar, Shreyas; Noguera, Daniel R.

    2010-01-01

    Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.

  11. mathFISH, a web tool that uses thermodynamics-based mathematical models for in silico evaluation of oligonucleotide probes for fluorescence in situ hybridization.

    Science.gov (United States)

    Yilmaz, L Safak; Parnerkar, Shreyas; Noguera, Daniel R

    2011-02-01

    Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.

  12. Standardization and optimization of fluorescence in situ hybridization (FISH) for HER-2 assessment in breast cancer: A single center experience.

    Science.gov (United States)

    Bogdanovska-Todorovska, Magdalena; Petrushevska, Gordana; Janevska, Vesna; Spasevska, Liljana; Kostadinova-Kunovska, Slavica

    2018-05-20

    Accurate assessment of human epidermal growth factor receptor 2 (HER-2) is crucial in selecting patients for targeted therapy. Commonly used methods for HER-2 testing are immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Here we presented the implementation, optimization and standardization of two FISH protocols using breast cancer samples and assessed the impact of pre-analytical and analytical factors on HER-2 testing. Formalin fixed paraffin embedded (FFPE) tissue samples from 70 breast cancer patients were tested for HER-2 using PathVysion™ HER-2 DNA Probe Kit and two different paraffin pretreatment kits, Vysis/Abbott Paraffin Pretreatment Reagent Kit (40 samples) and DAKO Histology FISH Accessory Kit (30 samples). The concordance between FISH and IHC results was determined. Pre-analytical and analytical factors (i.e., fixation, baking, digestion, and post-hybridization washing) affected the efficiency and quality of hybridization. The overall hybridization success in our study was 98.6% (69/70); the failure rate was 1.4%. The DAKO pretreatment kit was more time-efficient and resulted in more uniform signals that were easier to interpret, compared to the Vysis/Abbott kit. The overall concordance between IHC and FISH was 84.06%, kappa coefficient 0.5976 (p characteristics. Differences in the pre-analytical and analytical steps can affect the hybridization quality and efficiency. The use of DAKO pretreatment kit is time-saving and cost-effective.

  13. Metaphase FISH on a Chip: Miniaturized Microfluidic Device for Fluorescence in situ Hybridization

    DEFF Research Database (Denmark)

    Vedarethinam, Indumathi; Shah, Pranjul Jaykumar; Dimaki, Maria

    2010-01-01

    -FISH, the process continues to be a manual, labour intensive, expensive and time consuming technique, often taking over 3-5 days, even in dedicated labs. We have developed a novel microFISH device to perform metaphase FISH on a chip which overcomes many shortcomings of the current laboratory protocols. This work...... also introduces a novel splashing device for preparing metaphase spreads on a microscope glass slide, followed by a rapid adhesive tape-based bonding protocol leading to rapid fabrication of the microFISH device. The microFISH device allows for an optimized metaphase FISH protocol on a chip with over...... a 20-fold reduction in the reagent volume. This is the first demonstration of metaphase FISH on a microfluidic device and offers a possibility of automation and significant cost reduction of many routine diagnostic tests of genetic anomalies....

  14. Standardization and optimization of fluorescence in situ hybridization (FISH for HER-2 assessment in breast cancer: A single center experience

    Directory of Open Access Journals (Sweden)

    Magdalena Bogdanovska-Todorovska

    2018-05-01

    Full Text Available Accurate assessment of human epidermal growth factor receptor 2 (HER-2 is crucial in selecting patients for targeted therapy. Commonly used methods for HER-2 testing are immunohistochemistry (IHC and fluorescence in situ hybridization (FISH. Here we presented the implementation, optimization and standardization of two FISH protocols using breast cancer samples and assessed the impact of pre-analytical and analytical factors on HER-2 testing. Formalin fixed paraffin embedded (FFPE tissue samples from 70 breast cancer patients were tested for HER-2 using PathVysion™ HER-2 DNA Probe Kit and two different paraffin pretreatment kits, Vysis/Abbott Paraffin Pretreatment Reagent Kit (40 samples and DAKO Histology FISH Accessory Kit (30 samples. The concordance between FISH and IHC results was determined. Pre-analytical and analytical factors (i.e., fixation, baking, digestion, and post-hybridization washing affected the efficiency and quality of hybridization. The overall hybridization success in our study was 98.6% (69/70; the failure rate was 1.4%. The DAKO pretreatment kit was more time-efficient and resulted in more uniform signals that were easier to interpret, compared to the Vysis/Abbott kit. The overall concordance between IHC and FISH was 84.06%, kappa coefficient 0.5976 (p < 0.0001. The greatest discordance (82% between IHC and FISH was observed in IHC 2+ group. A standardized FISH protocol for HER-2 assessment, with high hybridization efficiency, is necessary due to variability in tissue processing and individual tissue characteristics. Differences in the pre-analytical and analytical steps can affect the hybridization quality and efficiency. The use of DAKO pretreatment kit is time-saving and cost-effective.

  15. Fluorescence in situ hybridization techniques (FISH) to detect changes in CYP19a gene expression of Japanese medaka (Oryzias latipes)

    International Nuclear Information System (INIS)

    Park, June-Woo; Tompsett, Amber; Zhang, Xiaowei; Newsted, John L.; Jones, Paul D.; Au, Doris; Kong, Richard; Wu, Rudolf S.S.; Giesy, John P.; Hecker, Markus

    2008-01-01

    The aim of this study was to develop a sensitive in situ hybridization methodology using fluorescence-labeled riboprobes (FISH) that allows for the evaluation of gene expression profiles simultaneously in multiple target tissues of whole fish sections of Japanese medaka (Oryzias latipes). To date FISH methods have been limited in their application due to autofluorescence of tissues, fixatives or other components of the hybridization procedure. An optimized FISH method, based on confocal fluorescence microscopy was developed to reduce the autofluorescence signal. Because of its tissue- and gender-specific expression and relevance in studies of endocrine disruption, gonadal aromatase (CYP19a) was used as a model gene. The in situ hybridization (ISH) system was validated in a test exposure with the aromatase inhibitor fadrozole. The optimized FISH method revealed tissue-specific expression of the CYP19a gene. Furthermore, the assay could differentiate the abundance of CYP19a mRNA among cell types. Expression of CYP19a was primarily associated with early stage oocytes, and expression gradually decreased with increasing maturation. No expression of CYP19a mRNA was observed in other tissues such as brain, liver, or testes. Fadrozole (100 μg/L) caused up-regulation of CYP19a expression, a trend that was confirmed by RT-PCR analysis on excised tissues. In a combination approach with gonad histology, it could be shown that the increase in CYP19a expression as measured by RT-PCR on a whole tissue basis was due to a combination of both increases in numbers of CYP19a-containing cells and an increase in the amount of CYP19a mRNA present in the cells

  16. Phytoplankton IF-FISH: Species-specific labeling of cellular proteins by immunofluorescence (IF) with simultaneous species identification by fluorescence immunohybridization (FISH).

    Science.gov (United States)

    Meek, Megan E; Van Dolah, Frances M

    2016-05-01

    Phytoplankton rarely occur as unialgal populations. Therefore, to study species-specific protein expression, indicative of physiological status in natural populations, methods are needed that will both assay for a protein of interest and identify the species expressing it. Here we describe a protocol for IF-FISH, a dual labeling procedure using immunofluorescence (IF) labeling of a protein of interest followed by fluorescence in situ hybridization (FISH) to identify the species expressing that protein. The protocol was developed to monitor expression of the cell cycle marker proliferating cell nuclear antigen (PCNA) in the red tide dinoflagellate, Karenia brevis, using a large subunit (LSU) rRNA probe to identify K. brevis in a mixed population of morphologically similar Karenia species. We present this protocol as proof of concept that IF-FISH can be successfully applied to phytoplankton cells. This method is widely applicable for the analysis of single-cell protein expression of any protein of interest within phytoplankton communities. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Fluorescence (FISH) and chromogenic (CISH) in situ hybridisation in prostate carcinoma cell lines: comparison and use of virtual microscopy.

    Science.gov (United States)

    Elliott, K; Hamilton, P W; Maxwell, P

    2008-01-01

    Chromogenic in situ hybridisation (CISH) has become an attractive alternative to fluorescence in situ hybridisation (FISH) due to its permanent stain which is more familiar to pathologists and because it can be viewed using light microscopy. The aim of the present study is to examine reproducibility in the assessment of abnormal chromosome number by CISH in comparison to FISH. Using three prostate cell lines--PNT1A (derived from normal epithelium), LNCAP and DU145 (derived from prostatic carcinoma), chromosomes 7 and 8 were counted in 40 nuclei in FISH preparations (x100 oil immersion) and 100 nuclei in CISH preparations (x40) by two independent observers. The CISH slides were examined using standard light microscopy and virtual microscopy. Reproducibility was examined using paired Student's t-test (PCISH. No significant differences in chromosome count were seen between the techniques. Chromosomes 7 and 8 showed disomic status for each cell line except LNCAP, which proved to be heterogeneous (disomic/aneusomic), particularly for chromosome 8. Virtual microscopy proved to be easy to use and gave no significant differences from standard light microscopy. These results support the hypothesis that there is no significant difference between FISH and CISH techniques.

  18. Komposisi dan Kelimpahan Ikan di Ekosistem Mangrove di Kedungmalang, Jepara (Fish Community Structure in Mangrove Ecosystem at Kedung Malang, Jepara Regency

    Directory of Open Access Journals (Sweden)

    Sri Redjeki

    2014-06-01

    Full Text Available Ekosistem mangrove di Kedungmalang, Kabupaten Jepara dilaporkan telah mengalami kerusakan ekologis. Kondisi ini mempengaruhi biota termasuk ikan yang hidup di kawasan tersebut. Penelitian ini dilakukan untuk mengetahui komposisi dan kelimpahan ikan di ekosistem mangrove di Desa Kedungmalang, Kecamatan Kedung, Kabupaten Jepara. Penelitian dilaksanakan bulan Mei sampai Agustus 2011. Pengambilan sampel ikan dilakukan di 3 lokasi perairan bervegetasi mangrove sejati (true mangrove Rhizophora sp. dan mangrove asosiasi (associate mangrove rumput Cyperus sp. Hasil penelitian menunjukkan terdapat 10 famili ikan, yaitu Mugilidae, Ariidae, Eleotridae, Pristigasteridae, Gobiidae, Drepanidae, Belonidae, Adrianichtyidae, Aplocheilidae, dan Haemulidae. Ikan paling banyak ditemui adalah famili Mugilidae, sedangkan ikan yang jarang ditemui adalah Famili Belonidae. Ikan Mugilidae hidup pada kisaran salinitas luas, sering masuk estuari dan sungai serta bersifat katadromous, biasanya membentuk kelompok besar di daerah dengan dasar pasir atau lumpur. Ikan famili Mugilidae yang paling banyak tertangkap adalah fase anakan dan juvenil. Jenis ikan Belanak ini merupakan ikan  yang berasosiasi dengan hutan mangrove selama periode anakan, tetapi saat dewasa cenderung menggerombol di sepanjang pantai berdekatan dengan hutan mangrove. Secara umum kelimpahan ikan pada saat surut selalu lebih tinggi dibandingkan saat pasang. Kelimpahan ikan di Rhizophora sp. lebih tinggi dibandingkan Cyperus sp. baik pada saat surut maupun pasang. Kata kunci: ekosistem mangrove, ikan, komposisi, Mugillidae Mangrove ecosystem at Kedungmalang has been reported experiencing  an ecological damage. This condition directly or indirectly affect the organisms, including fish that live around the area ecosystem. The purpose of this study was to identify and determine the abundance the fish communities in mangrove ecosystem in the Kedungmalang Village, Kedung District, Jepara Regency. The research

  19. PENINDAKAN ILLEGAL FISHING DAN PERJANJIAN BILATERAL BIDANG PERIKANAN DENGAN NEGARA TETANGGA

    Directory of Open Access Journals (Sweden)

    Ms. Koesrianti

    2008-06-01

    Full Text Available Indonesia is the biggest maritim state in the world as two third of its area are ocean. In order to maximized its fisheries products, Indonesia has some policies regarding to the management of its waters, including eradicating illegal logging and reforming bilateral agreements with some neighbor countries. This policies, however, appear to be not yet optimal. The government in 1985 has ratified the UN Convention on the Law of the Sea (UNCLOS 1982 with the Act 17/1985. However, up to now, Indonesia has not ratified yet the United Nations Fish Stocks Agreement 1995 and the 1993 Compliance Agreement as the continued arrangements of UNCLOS 1982. Indonesia should conduct such ratification in order to optimize the Indonesian fisheries industry in which its natural resources, whether living or non-living can be explored and exploited. This is crucial as fisheries industries can provide fish stock as alternative healthy food for the people in Indonesia.

  20. QUANTITATIVE IMAGING AND STATISTICAL ANALYSIS OF FLUORESCENCE IN SITU HYBRIDIZATION (FISH) OF AUREOBASIDIUM PULLULANS. (R823845)

    Science.gov (United States)

    AbstractImage and multifactorial statistical analyses were used to evaluate the intensity of fluorescence signal from cells of three strains of A. pullulans and one strain of Rhodosporidium toruloides, as an outgroup, hybridized with either a universal o...

  1. Rapid molecular cytogenetic analysis of X-chromosomal microdeletions: Fluorescence in situ hybridization (FISH) for complex glycerol kinase deficiency

    Energy Technology Data Exchange (ETDEWEB)

    Worley, K.C.; Lindsay, E.A.; McCabe, E.R.B. [Baylor College of Medicine, Houston, TX (United States)] [and others

    1995-07-17

    Diagnosis of X-chromosomal microdeletions has relied upon the traditional methods of Southern blotting and DNA amplification, with carrier identification requiring time-consuming and unreliable dosage calculations. In this report, we describe rapid molecular cytogenetic identification of deleted DNA in affected males with the Xp21 contiguous gene syndrome (complex glycerol kinase deficiency, CGKD) and female carriers for this disorder. CGKD deletions involve the genes for glycerol kinase, Duchenne muscular dystrophy, and/or adrenal hypoplasia congenita. We report an improved method for diagnosis of deletions in individuals with CGKD and for identification of female carriers within their families using fluorescence in situ hybridization (FISH) with a cosmid marker (cosmid 35) within the glycerol kinase gene. When used in combination with an Xq control probe, affected males demonstrate a single signal from the control probe, while female carriers demonstrate a normal chromosome with two signals, as well as a deleted chromosome with a single signal from the control probe. FISH analysis for CGKD provides the advantages of speed and accuracy for evaluation of submicroscopic X-chromosome deletions, particularly in identification of female carriers. In addition to improving carrier evaluation, FISH will make prenatal diagnosis of CGKD more readily available. 17 refs., 2 figs.

  2. Cytogenetic analysis using fluorescence in situ hybridization (FISH) to evaluate occupational exposure to carcinogens

    Czech Academy of Sciences Publication Activity Database

    Šrám, Radim; Beskid, Olena; Binková, Blanka; Rössner, P.; Šmerhovský, Zdeněk

    2004-01-01

    Roč. 149, - (2004), s. 335-344 ISSN 0378-4274 R&D Projects: GA MŽP SI/340/2/00 Institutional research plan: CEZ:AV0Z5039906 Keywords : Chromosomal aberrations * Fluorescence in situ hybridization Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 2.571, year: 2004

  3. Fluctuation localization imaging-based fluorescence in situ hybridization (fliFISH) for accurate detection and counting of RNA copies in single cells

    Energy Technology Data Exchange (ETDEWEB)

    Cui, Yi; Hu, Dehong; Markillie, Lye Meng; Chrisler, William B.; Gaffrey, Matthew J.; Ansong, Charles; Sussel, Lori; Orr, Galya

    2017-10-04

    Quantitative gene expression analysis in intact single cells can be achieved using single molecule- based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple FISH sub-probes, and background noise. Thus, the precision in smFISH is often compromised by partial or nonspecific binding of sub-probes and tissue autofluorescence, limiting its accuracy. Here we provide an accurate approach for setting quantitative thresholds between true and false signals, which relies on blinking frequencies of photoswitchable dyes. This fluctuation localization imaging-based FISH (fliFISH) uses blinking frequency patterns, emitted from a transcript bound to multiple sub-probes, which are distinct from blinking patterns emitted from partial or nonspecifically bound sub-probes and autofluorescence. Using multicolor fliFISH, we identified radial gene expression patterns in mouse pancreatic islets for insulin, the transcription factor, NKX2-2, and their ratio (Nkx2-2/Ins2). These radial patterns, showing higher values in β cells at the islet core and lower values in peripheral cells, were lost in diabetic mouse islets. In summary, fliFISH provides an accurate, quantitative approach for detecting and counting true RNA copies and rejecting false signals by their distinct blinking frequency patterns, laying the foundation for reliable single-cell transcriptomics.

  4. Fish samples as bioindicator of environmental quality: synchrotron radiation total reflection X-ray fluorescence analysis (SR-TXRF)

    International Nuclear Information System (INIS)

    Vives, Ana Elisa Sirito de; Moreira, Silvana; Brienza, Sandra Maria Boscolo; Zucchi, Orgheda Luiza Araujo Domingues; Nascimento Filho, Virgilio Franco do Centro de Energia Nuclear na Agricultura , Piracicaba, SP; Brazil)

    2005-01-01

    In this study fish were used as bioindicators of environmental contamination. The species were collected in Piracicaba River, Sao Paulo state, Brazil and the toxic elements concentrations were determined in muscle tissue and viscus (liver, intestine and stomach) by Synchrotron Radiation Total Reflection X-Ray Fluorescence Analysis (SR-TXRF). Were determined the elements Ti, Cr, Mn, Fe, Ni, Cu, Zn and Ba. The results were compared with values established by Brazilian Legislation for general food. The elements concentrations evidenced potential risk to human health and environmental quality alteration of the studied area. The measurements were realized at the 'Laboratorio Nacional de Luz Sincrotron' (LNLS) located in Campinas, Sao Paulo State, Brazil. (author)

  5. Fish samples as bioindicator of environmental quality: synchrotron radiation total reflection X-ray fluorescence analysis (SR-TXRF)

    Energy Technology Data Exchange (ETDEWEB)

    Vives, Ana Elisa Sirito de [Universidade Metodista de Piracicaba (UNIMEP), Santa Barbara D' Oeste, SP (Brazil). Faculdade de Engenharia, Arquitetura e Urbanismo]. E-mail: aesvives@unimep.br; Moreira, Silvana [Universidade Estadual de Campinas, SP (Brazil). Faculdade de Engenharia, Arquitetura e Urbanismo]. E-mail: Silvana@fec.unicamp.br; Brienza, Sandra Maria Boscolo [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil). Faculdade de Ciencias Matematicas, da Natureza e de Tecnologia da Informacao]. E-mail: sbrienza@unimep.br; Zucchi, Orgheda Luiza Araujo Domingues [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas]. E-mail: olzucchi@fcfrp.usp.br; Nascimento Filho, Virgilio Franco do [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)]. E-mail: virgilio@cena.usp.br

    2005-07-01

    In this study fish were used as bioindicators of environmental contamination. The species were collected in Piracicaba River, Sao Paulo state, Brazil and the toxic elements concentrations were determined in muscle tissue and viscus (liver, intestine and stomach) by Synchrotron Radiation Total Reflection X-Ray Fluorescence Analysis (SR-TXRF). Were determined the elements Ti, Cr, Mn, Fe, Ni, Cu, Zn and Ba. The results were compared with values established by Brazilian Legislation for general food. The elements concentrations evidenced potential risk to human health and environmental quality alteration of the studied area. The measurements were realized at the 'Laboratorio Nacional de Luz Sincrotron' (LNLS) located in Campinas, Sao Paulo State, Brazil. (author)

  6. Validation of interphase fluorescence in situ hybridization (iFISH for multiple myeloma using CD138 positive cells

    Directory of Open Access Journals (Sweden)

    Renata Kiyomi Kishimoto

    2016-06-01

    Full Text Available ABSTRACT BACKGROUND: Multiple myeloma is a plasma cell neoplasm with acquired genetic abnormalities of clinical and prognostic importance. Multiple myeloma differs from other hematologic malignancies due to a high fraction of low proliferating malignant plasma cells and the paucity of plasma cells in bone marrow aspiration samples, making cytogenetic analysis a challenge. An abnormal karyotype is found in only one-third of patients with multiple myeloma and interphase fluorescence in situ hybridization is the most useful test for studying the chromosomal abnormalities present in almost 90% of cases. However, it is necessary to study the genetic abnormalities in plasma cells after their identification or selection by morphology, immunophenotyping or sorting. Other challenges are the selection of the most informative FISH panel and determining cut-off levels for FISH probes. This study reports the validation of interphase fluorescence in situ hybridization using CD138 positive cells, according to proposed guidelines published by the European Myeloma Network (EMN in 2012. METHOD: Bone marrow samples from patients with multiple myeloma were used to standardize a panel of five probes [1q amplification, 13q14 deletion, 17p deletion, t(4;14, and t(14;16] in CD138+ cells purified by magnetic cell sorting. RESULTS: This test was validated with a low turnaround time and good reproducibility. Five of six samples showed genetic abnormalities. Monosomy/deletion 13 plus t(4;14 were found in two cases. CONCLUSION: This technique together with magnetic cell sorting is effective and can be used in the routine laboratory practice. In addition, magnetic cell sorting provides a pure plasma cell population that allows other molecular and genomic studies.

  7. Feasibility of using fluorescence in situ hybridization (FISH) to detect early gene changes in sputum cells from uranium miners

    Energy Technology Data Exchange (ETDEWEB)

    Neft, R.E.; Rogers, J.L.; Belinsky, S.A. [and others

    1995-12-01

    Epidemiological studies have shown that combined exposure to radon progeny and tobacco smoke produce a greater than additive or synergistic increase in lung cancer risk. Lung cancer results from multiple genetic changes over a long period of time. An early change that occurs in lung cancer is trisomy 7 which is found in 50% of non-small cell lung cancer and in the far margins of resected lung tumors. The 80% mortality associated with lung cancer is in part related to the high proportion of patients who present with an advanced, unresectable tumor. Therefore, early detection of patients at risk for tumor development is critical to improve treatment of this disease. Currently, it is difficult to detect lung cancer early while it is still amendable by surgery. Saccomanno, G. has shown that premalignant cytologic changes in sputum cells collected from uranium miners can be detected by a skilled, highly trained cytopathologist. A more objective alternative for identifying premalignant cells in sputum may be to determine whether an early genetic change such as trisomy 7 is present in these cells. Fluorescence in situ hybridization (FISH) can be used to identify cells with trisomy 7. The results of this investigation indicate that FISH may prove to be an accurate, efficient method to test at-risk individuals for genetic alterations in bronchial epithelial cells from sputum.

  8. Feasibility of using fluorescence in situ hybridization (FISH) to detect early gene changes in sputum cells from uranium miners

    International Nuclear Information System (INIS)

    Neft, R.E.; Rogers, J.L.; Belinsky, S.A.

    1995-01-01

    Epidemiological studies have shown that combined exposure to radon progeny and tobacco smoke produce a greater than additive or synergistic increase in lung cancer risk. Lung cancer results from multiple genetic changes over a long period of time. An early change that occurs in lung cancer is trisomy 7 which is found in 50% of non-small cell lung cancer and in the far margins of resected lung tumors. The 80% mortality associated with lung cancer is in part related to the high proportion of patients who present with an advanced, unresectable tumor. Therefore, early detection of patients at risk for tumor development is critical to improve treatment of this disease. Currently, it is difficult to detect lung cancer early while it is still amendable by surgery. Saccomanno, G. has shown that premalignant cytologic changes in sputum cells collected from uranium miners can be detected by a skilled, highly trained cytopathologist. A more objective alternative for identifying premalignant cells in sputum may be to determine whether an early genetic change such as trisomy 7 is present in these cells. Fluorescence in situ hybridization (FISH) can be used to identify cells with trisomy 7. The results of this investigation indicate that FISH may prove to be an accurate, efficient method to test at-risk individuals for genetic alterations in bronchial epithelial cells from sputum

  9. Fluorescent in situ hybridization (FISH) on corneal impression cytology specimens (CICS): study of epithelial cell survival after keratoplasty.

    Science.gov (United States)

    Catanese, Muriel; Popovici, Cornel; Proust, Hélène; Hoffart, Louis; Matonti, Frédéric; Cochereau, Isabelle; Conrath, John; Gabison, Eric E

    2011-02-22

    To assess corneal epithelial cell survival after keratoplasty. Corneal impression cytology (CIC) was performed on sex-mismatched corneal transplants. Fluorescent in situ hybridization (FISH) with sex chromosome-specific probes was performed to identify epithelial cell mosaicism and therefore allocate the donor or recipient origin of the cells. Twenty-four samples of corneal epithelial cells derived from 21 transplanted patients were analyzed. All patients received post-operative treatment using dexamethasone eye drops, with progressive tapering over 18 months, and nine patients also received 2% cyclosporine eye drops. Out of the 24 samples reaching quality criteria, sex mosaicism was found in 13, demonstrating the presence of donor-derived cells at the center of the graft for at least 211 days post keratoplasty. Kaplan-Meier analysis established a median survival of donor corneal epithelial cells of 385 days. Although not statistically significant, the disappearance of donor cells seemed to be delayed and the average number of persistent cells appeared to be greater when 2% cyclosporine was used topically as an additional immunosuppressive therapy. The combination of corneal impressions and FISH analysis is a valuable tool with negligible side effects to investigate the presence of epithelial cell mosaicism in sex-mismatched donor transplants. Epithelial cells survived at the center of the graft with a median survival of more than one year, suggesting slower epithelial turnover than previously described.

  10. The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa

    Czech Academy of Sciences Publication Activity Database

    Flajšhans, Martin; Cosson, J.; Rodina, Marek; Linhart, Otomar

    2004-01-01

    Roč. 28, č. 12 (2004), s. 955-959 ISSN 1065-6995 R&D Projects: GA MŠk ME 638; GA ČR GA524/03/0178; GA AV ČR KSK6005114 Institutional research plan: CEZ:AV0Z5045916 Keywords : image cytometry * dual fluorescent * staining Subject RIV: ED - Physiology Impact factor: 1.015, year: 2004

  11. Human exposure to mercury in a compact fluorescent lamp manufacturing area: By food (rice and fish) consumption and occupational exposure

    International Nuclear Information System (INIS)

    Liang, Peng; Feng, Xinbin; Zhang, Chan; Zhang, Jin; Cao, Yucheng; You, Qiongzhi; Leung, Anna Oi Wah; Wong, Ming-Hung; Wu, Sheng-Chun

    2015-01-01

    To investigate human Hg exposure by food consumption and occupation exposure in a compact fluorescent lamp (CFL) manufacturing area, human hair and rice samples were collected from Gaohong town, Zhejiang Province, China. The mean values of total mercury (THg) and methylmercury (MeHg) concentrations in local cultivated rice samples were significantly higher than in commercial rice samples which indicated that CFL manufacturing activities resulted in Hg accumulation in local rice samples. For all of the study participants, significantly higher THg concentrations in human hair were observed in CFL workers compared with other residents. In comparison, MeHg concentrations in human hair of residents whose diet consisted of local cultivated rice were significantly higher than those who consumed commercial rice. These results demonstrated that CFL manufacturing activities resulted in THg accumulation in the hair of CFL workers. However, MeHg in hair were mainly affected by the sources of rice of the residents. - Highlights: • Rice samples were contaminated by Compact fluorescent lamp (CFL) manufacturing. • CFL manufacturing lead to THg accumulation in human hair. • MeHg in human hair were mainly affected by the sources of rice. • MeHg intake from fish consumption was lower than that from rice consumption. • PDI of MeHg by food consumption was below the guidelines for public health concern. - CFL manufacturing activities result in Hg accumulation in local rice samples and hair of CFL workers. However, MeHg in hair were mainly affected by sources of rice

  12. Apportioning bacterial carbon source utilization in soil using 14 C isotope analysis of FISH-targeted bacterial populations sorted by fluorescence activated cell sorting (FACS): 14 C-FISH-FACS.

    Science.gov (United States)

    Gougoulias, Christos; Meade, Andrew; Shaw, Liz J

    2018-02-19

    An unresolved need in microbial ecology is methodology to enable quantitative analysis of in situ microbial substrate carbon use at the population level. Here, we evaluated if a novel combination of radiocarbon-labelled substrate tracing, fluorescence in situ hybridisation (FISH) and fluorescence-activated cell sorting (FACS) to sort the FISH-targeted population for quantification of incorporated radioactivity ( 14 C-FISH-FACS) can address this need. Our test scenario used FISH probe PSE1284 targeting Pseudomonas spp. (and some Burkholderia spp.) and salicylic acid added to rhizosphere soil. We examined salicylic acid- 14 C fate (mineralized, cell-incorporated, extractable and non-extractable) and mass balance (0-24 h) and show that the PSE1284 population captured ∼ 50% of the Nycodenz extracted biomass 14 C. Analysis of the taxonomic distribution of the salicylic acid biodegradation trait suggested that PSE1284 population success was not due to conservation of this trait but due to competitiveness for the added carbon. Adding 50KBq of 14 C sample -1 enabled detection of 14 C in the sorted population at ∼ 60-600 times background; a sensitivity which demonstrates potential extension to analysis of rarer/less active populations. Given its sensitivity and compatibility with obtaining a C mass balance, 14 C-FISH-FACS allows quantitative dissection of C flow within the microbial biomass that has hitherto not been achieved. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.

  13. Fish and Female Agency in a Madurese Fishing Village in Indonesia Poissardes et poissons à Madura. Femmes en action dans un village de pêcheurs d’Indonésie

    Directory of Open Access Journals (Sweden)

    Anke Niehof

    2012-12-01

    Full Text Available Le rôle crucial des femmes dans l’économie halieutique locale et leur haut degré d’autonomie sociale constituaient deux traits remarquables de l’organisation socio-économique du village de pêcheurs de Patondu (Madura en 1978. Un nouveau travail de terrain en 2004 montre que se sont produits au cours de ces 26 ans dans la pêcherie des changements technologiques et économiques de grande portée. De plus, avec la mise en place d’équipements publics, Patondu est désormais mieux relié au monde extérieur. En 2004, pourtant, les femmes y ont, autant sinon plus qu’avant, une place éminente. Les cas de six femmes actives dans le commerce et le traitement du poisson et le financement de la pêche sont examinés. Les pangamba’, considérées dans la littérature comme formant « un groupe phénoménal » , méritent une attention particulière : ces femmes – marchandes, entrepreneures et banquières, tout à la fois – sont les pivots de l’économie halieutique, comme « matrones » dans les relations de « matronage » qui structurent les réseaux socio-économiques. La solide position des femmes repose sur une division sexuelle du travail, écologiquement et culturellement fondée dans la société de Patondu, qui leur ouvre un vaste champ d’opportunités d’action dans les sphères économique et sociale. La vie quotidienne de Patondu s’apprécie en ichtyo-valeur et les femmes déterminent cette valeur. Elles contrôlent donc, dans une large mesure, l’économie halieutique locale. De plus, la limite fluide entre les sphères domestique et économique évite aux femmes le confinement au foyer et leur permet de jouer ce rôle de matrones dans la sphère économique. Des recherches sur les commerçantes des marchés à Java ont produit des résultats similaires. La société de Patondu n’est pas égalitaire et, même si le genre, comme facteur de stratification, recoupe d’autres facteurs et si le hiatus

  14. Enhancement of photorepair of ultraviolet-damage by preillumination with fluorescent light in cultured fish cells

    Energy Technology Data Exchange (ETDEWEB)

    Yasuhira, Shinji; Mitani, Hiroshi; Shima, Akihiro (Tokyo Univ. (Japan). Faculty of Science)

    1991-02-01

    Fluorescent light (FL) illumination of RBCF-1 cells derived from a goldfish, prior to 254 nm UV-irradiation enhanced their ability to photorepair. Cells were illuminated with FL for 1 h (29 W/m{sup 2}) and incubated for 8 h in the dark before being irradiated with 10 J/m{sup 2} UV. The surviving fraction of FL-treated cells after UV-irradiation rose about 7-fold (from 3 to 20%) by 20 min photorepair treatment with the same FL source, and 4-fold (from 1.6 to 6%) in the FL non-treated cells. Flow cytometric analysis showed that FL treatment did not affect the distribution of cell cycle phase at the time of UV-irradiation (8 h after FL treatment). Pyrimidine dimers induced by UV were measured by the use of UV endonuclease of micrococcus luteus and alkaline agarose gel electrophoresis. Initial yields of dimers after exposure to 10 J/m{sup 2} UV were almost the same (about 0.11 dimer/kb) between FL treated and non-treated cells. After 20 min photorepair treatment, {similar to} 70% of dimers were removed in the FL treated samples, while < 20% were removed in the non FL-treated ones. (author).

  15. Determination of total and inorganic mercury in fish samples with on-line oxidation coupled to atomic fluorescence spectrometry

    International Nuclear Information System (INIS)

    Shao Lijun; Gan Wuer; Su Qingde

    2006-01-01

    An atomic fluorescence spectrometry system for determination of total and inorganic mercury with electromagnetic induction-assisted heating on-line oxidation has been developed. Potassium peroxodisulphate was used as the oxidizing agent to decompose organomercury compounds. Depending on the temperature selected, inorganic or total mercury could be determined with the same manifold. Special accent was put on the study of the parameters influencing the on-line digestion efficiency. The tolerance to the interference of coexisting ions was carefully examined in this system. Under optimal conditions, the detection limits (3σ) were evaluated to be 2.9 ng l -1 for inorganic mercury and 2.6 ng l -1 for total mercury, respectively. The relative standard deviations for 10 replicate determinations of 1.0 μg l -1 Hg were 2.4 and 3.2% for inorganic mercury and total mercury, respectively. The proposed method was successfully applied to the determination of total and inorganic mercury in fish samples

  16. Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes.

    Science.gov (United States)

    Chae, Kyu-Jung; Rameshwar, T; Jang, Am; Kim, Sung H; Kim, In S

    2008-09-01

    There is growing interest in the development of more cost-effective and retrofit technologies for the upgrade and expansion of existing wastewater treatment plants with extreme space constraints. A free-floating sponge media (BioCube) process, using a 24 L lab scale reactor, was operated to study the nitrification profiles and microbial community. The COD removal efficiencies were maintained, at an average of 95%, with the mixed liquor suspended solids (MLSS) inside the BioCube sponge media maintained at 12,688 mg/L. The nitrification removal efficiencies were between 92% and 100%, with an average value of 99%. From the results of microelectrode measurements, the ammonium ion concentration was found to rapidly decrease from the surface of the BioCube sponge media to a depth of 2mm due to chemical reactions carried out by ammonia oxidizing bacteria (AOB) species. Multi-fluorescence in situ hybridization (FISH) has been used to investigate the spatial distributions of various microbial activities within reactors. Microbial communities were targeted using different oligonucleotide probes specific to AOB and nitrite oxidizing bacteria (NOB). There were a large number of AOB populations, but these were not uniformly distributed in the biofilm compared to the NOB populations.

  17. Utility of chromogenic in situ hybridization (CISH) for detection of EGFR amplification in glioblastoma: comparison with fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Fischer, Ingeborg; de la Cruz, Clarissa; Rivera, Andreana L; Aldape, Kenneth

    2008-12-01

    In this study, we test the reliability of chromogenic in situ hybridization (CISH) for the detection of epidermal growth factor receptor (EGFR) gene amplification in glioblastoma. Earlier reports have described EGFR CISH in glioblastoma multiforme, but a comparison of CISH with a "gold standard" testing method, such as fluorescence in situ hybridization (FISH), has not been described. Therapies targeting the EGFR-signaling pathway might increase the importance of assessment of EGFR-amplification status. CISH is a potential alternative to FISH as a testing method. To test its reliability, EGFR-amplification status by CISH was assessed in 89 cases of glioblastoma and compared with FISH results, and correlated with the protein expression using immunohistochemistry (IHC) for EGFR. FISH was scored as being EGFR-amplified in 47/89 tumors, CISH as being amplified in 43/89 tumors. The CISH and FISH results were in agreement in 83/89 cases (93%). Four glioblastomas were scored as being amplified by FISH, but not by CISH; whereas amplification was detected in 2 tumors by CISH that were not amplified using FISH. Forty-eight of the 89 cases were positive for EGFR expression by IHC. EGFR amplification was highly correlated with protein expression by IHC, as 40/48 (83%) EGFR IHC-positive cases were found to be EGFR-amplified. The high concordance of CISH and FISH for the assessment of EGFR gene-amplification status indicates that CISH is a viable alternative to FISH for the detection of EGFR gene amplification in glioblastoma. Detectable EGFR expression by IHC can occur in the absence of gene amplification, but is uncommon.

  18. Do the fluorescent red eyes of the marine fish Tripterygion delaisi stand out? In situ and in vivo measurements at two depths.

    Science.gov (United States)

    Harant, Ulrike K; Santon, Matteo; Bitton, Pierre-Paul; Wehrberger, Florian; Griessler, Thomas; Meadows, Melissa G; Champ, Connor M; Michiels, Nico K

    2018-05-01

    Since the discovery of red fluorescence in fish, much effort has been invested to elucidate its potential functions, one of them being signaling. This implies that the combination of red fluorescence and reflection should generate a visible contrast against the background. Here, we present in vivo iris radiance measurements of Tripterygion delaisi under natural light conditions at 5 and 20 m depth. We also measured substrate radiance of shaded and exposed foraging sites at those depths. To assess the visual contrast of the red iris against these substrates, we used the receptor noise model for chromatic contrasts and Michelson contrast for achromatic calculations. At 20 m depth, T. delaisi iris radiance generated strong achromatic contrasts against substrate radiance, regardless of exposure, and despite substrate fluorescence. Given that downwelling light above 600 nm is negligible at this depth, we can attribute this effect to iris fluorescence. Contrasts were weaker in 5 m. Yet, the pooled radiance caused by red reflection and fluorescence still exceeded substrate radiance for all substrates under shaded conditions and all but Jania rubens and Padina pavonia under exposed conditions. Due to the negative effects of anesthesia on iris fluorescence, these estimates are conservative. We conclude that the requirements to create visual brightness contrasts are fulfilled for a wide range of conditions in the natural environment of T. delaisi .

  19. KANDUNGAN VITAMIN B6, B9, B12 DAN E BEBERAPA JENIS DAGING, TELUR, IKAN DAN UDANG LAUT DI BOGOR DAN SEKITARNYA (VITAMIN B6, B9, B12 AND E CONTENT OF SEVERAL TYPES OF MEATS, EGGS, FISHES AND MARINE SHRIMPS IN BOGOR AND SURROUNDING AREAS

    Directory of Open Access Journals (Sweden)

    Heru Yuniati

    2012-06-01

    Full Text Available ABSTRACT Food Composition Table (DKBM in Indonesia has not mentioned all types of nutrients available in the food, particularly vitamin B6, B9 (folic acid, B12, and vitamin E. Therefore this study aimed to analyze the content of vitamin B6, B9 (folic acid, B12, and vitamin E in several types of meat, eggs, fish and marine shrimps consumed in Bogor and surrounding areas. Vitamin B6, B9, B12, and vitamin E from three kinds of meat (chicken, beef, lamb, two types of eggs (chicken, duck, and four species of fish (snapper, bloating, carp and tuna and crayfish are analyzed using High-Performance Liquid Chromatography (HPLC. The samples used are raw and taken from three locations in Bogor and surrounding areas. Fishes, meats and eggs contain high levels of folic acid, however the amount of folic acid content in meat varies depending on which part of meat the samples are taken, types of organ, and the fat content of the meat. The folic acid content in chicken wings is different with those in thigh. In fatty mutton the folic acid is higher than in those lean meat, and in yolk is higher than those in egg white. Vitamin E content of snapper is the highest amongs other types of fishes (6.54 µg/100 g.Chicken eggs contain a higher amount of vitamin E than duck eggs, while the yolk contains ahigher amount of vitamin E than those egg white. Keywords: animal foods, vitamin B6, vitamin B9 (folic Acid, vitamin B12, vitamin E   ABSTRAK Daftar Komposisi Bahan Makanan (DKBM yang ada di Indonesia belum memuat semua jenis zat gizi dalam makanan, khususnya vitamin B6, B9 (asam folat, B12 dan vitamin E. Menganalisis kandungan vitamin B6, B9 (asam folat, B12, dan vitamin E dalam beberapa jenis daging, telur, ikan dan udang laut yang dikonsumsi masyarakat di Bogor dan sekitarnya. Kandungan vitamin B6, B9, B12 dan vitamin E dari tiga jenis daging (ayam, sapi, kambing, dua jenis telur (ayam, itik, serta empat jenis ikan (kakap, kembung, mas, tongkol dan udang laut

  20. Tubular and endothelial chimerism in renal allografts using fluorescence and chromogenic in situ hybridization (FISH, CISH) technology.

    Science.gov (United States)

    Varga, Zsuzsanna; Gaspert, Ariana; Behnke, Silvia; von Teichman, Adriana; Fritzsche, Florian; Fehr, Thomas

    2012-04-01

    The role of endothelial and tubular chimerism in renal allograft adaptation and rejection varies in different studies. We addressed the correlation between different clinico-pathological settings and sex-chromosomal endothelial and/or tubular chimerism in renal allografts. We examined the presence or absence of the X and Y chromosomes by fluorescence and chromogenic in situ hybridization (FISH, CISH) methodology on paraffin embedded kidney biopsies in 16 gender mismatched renal transplants (1 to 12 years post-transplantation). Twelve patients were male, four female. Four groups were selected: (i) Vascular calcineurin inhibitor toxicity without rejection; (ii) T-cell mediated vascular rejection; (iii) antibody mediated rejection; and (iv) C4d-positivity in AB0-incompatible transplants with or without rejection. Twelve non-transplant kidney biopsies (8 female, 4 male) were used as controls. Tubular chimerism was detected more frequently (69%) than endothelial chimerism (12%) in renal transplants. One of 12 control patients had tubular and endothelial chimeric cells (8%). The Y chromosome occurred in 8/12 male recipients (67%) in tubular epithelial cells and in 5/12 male recipients (42%) in endothelial cells. Double X chromosomes were detected in 3/4 female recipients in tubular epithelium. Tubular chimerism occurred more often with endothelial chimerism and capillaritis without correlation with other parameters, such as rejection. Combined Y chromosomal tubular and lymphatic endothelial chimerism correlated with T-cell mediated vascular rejection in two out of three patients (66%). Combined Y chromosomal tubular and peritubular capillary chimerism correlated with antibody mediated C4d+ rejection in one out of two patients (50%). Tubular and/or endothelial chimerism occur frequently in gender mismatched renal allografts and, when combined, this is associated with T-cell mediated rejection. © 2012 The Authors. Pathology International © 2012 Japanese Society of

  1. HER-2 gene amplification by chromogenic in situ hybridisation (CISH) compared with fluorescence in situ hybridisation (FISH) in breast cancer-A study of two hundred cases.

    Science.gov (United States)

    Sáez, A; Andreu, F J; Seguí, M A; Baré, M L; Fernández, S; Dinarés, C; Rey, M

    2006-08-01

    The purpose of the study was to compare two methods used to analyse HER-2 gene amplification (fluorescence in situ hybridisation (FISH) and chromogenic in situ hybridisation (CISH)), and determine the accuracy of the antibodies CB11 and HercepTest for immunohistochemical detection of HER-2 overexpression from archival breast cancer tissue. Additionally, interobserver variability in the interpretation of CISH and immunohistochemical tests was measured. Two hundred cases of invasive breast carcinoma diagnosed between 2000 and 2003 were selected. Immunohistochemistry (IHC) was performed with HercepTest and CB11, and gene amplification was determined by FISH (PathVision, Vysis) and CISH (Zymed) using tissue macroarrays. An excellent concordance (94.8%) was found between CISH and FISH. Considering FISH as gold standard, sensitivity of CISH was 97.5% and specificity 94%. Overall interobserver agreement of CISH was 97.5% and of IHC 84%. Both antibodies showed a sensitivity of 95.2% and a specificity of 70.7% (CB11) and 81.2% (HercepTest). Our results show that CISH is a highly accurate, reproducible and practical technique to determine HER-2 gene amplification. CB11 and HercepTest are good screening methods with a high sensitivity. The performance of tissue macroarrays to test HER-2 status by IHC, FISH and CISH has demonstrated to be an available and effective method to study large series of tumours.

  2. Detection of denitrification genes by in situ rolling circle amplification - fluorescence in situ hybridization (in situ RCA-FISH) to link metabolic potential with identity inside bacterial cells

    DEFF Research Database (Denmark)

    Hoshino, Tatsuhiko; Schramm, Andreas

    2010-01-01

    target site. Finally, the RCA product inside the cells was detected by standard fluorescence in situ hybridization (FISH). The optimized protocol showed high specificity and signal-to-noise ratio but low detection frequency (up to 15% for single-copy genes and up to 43% for the multi-copy 16S rRNA gene...... as Candidatus Accumulibacter phosphatis by combining in situ RCA-FISH with 16S rRNA-targeted FISH. While not suitable for quantification because of its low detection frequency, in situ RCA-FISH will allow to link metabolic potential with 16S rRNA (gene)-based identification of single microbial cells.......). Nevertheless, multiple genes (nirS and nosZ; nirS and the 16S rRNA gene) could be detected simultaneously in P. stutzeri. Environmental application of in situ RCA-FISH was demonstrated on activated sludge by the differential detection of two types of nirS-defined denitrifiers; one of them was identified...

  3. Le diagnostic anténatal de la trisomie 21 par l'hybridation in situ en fluorescence (FISH): à propos des premiers tests réalisés au Maroc

    Science.gov (United States)

    Lamzouri, Afaf; Natiq, Abdelhafid; Tajir, Mariam; Sendid, Mohamed; Sefiani, Abdelaziz

    2012-01-01

    Introduction Le but de cette étude était de présenter les premiers résultats de diagnostic anténatal de la trisomie 21 par la technique d'hybridation in situ en fluorescence (FISH) au Maroc et discuter son intérêt dans le diagnostic rapide de cette aneuploïdie. Méthodes Ce travail a été réalisé chez 23 femmes avec des grossesses à haut risque de trisomie 21. La moyenne d’âge des gestantes étaient de 37,43 ans avec des extrêmes de 21 et 43 ans. Toutes étaient musulmanes mariées, mariage légitimé par la Charia, dont trois mariages consanguins, sauf une originaire de la République Démocratique du Congo qui était chrétienne et concubine. La majorité des femmes étaient fonctionnaires et avaient un niveau de scolarisation moyen à élevé. Toutes les patientes ont bénéficié d'une consultation de génétique médicale au cours de laquelle il leur a été donné des informations sur la technique, son intérêt et ses limites. Il s'agit de femmes enceintes qui avaient soit un âge maternel élevé ou des signes d'appel échographiques et/ ou biochimiques. Une des patientes était porteuse d'une translocation robertsonienne t(14;21) équilibrée. Une amniocentèse a été réalisée chez toutes les gestantes et aucun avortement n'a était induit par ce geste invasif. L’âge gestationnel moyen à la première consultation était de 14 semaines d'aménorrhée (SA) et à l'amniocentèse était de 16 SA et 5 jours. L'analyse FISH a été réalisée, après consentement des couples, sur des cellules non cultivées à partir des échantillons de liquides amniotiques, en utilisant des sondes spécifiques du chromosome 21. Résultats Parmi les 23 patientes qui ont bénéficiées d'un diagnostic anténatal de la trisomie 21 par la technique FISH, nous avons pu rassurer 21 d'entre elles, et nous avons détecté deux cas de trisomie 21 fœtal. Conclusion La technique FISH permet un diagnostic anténatal rapide, en moins de 48h, de la trisomie 21 sur

  4. Detection of malachite green in fish based on magnetic fluorescent probe of CdTe QDs/nano-Fe3O4@MIPs

    Science.gov (United States)

    Wu, Le; Lin, Zheng-Zhong; Zeng, Jun; Zhong, Hui-Ping; Chen, Xiao-Mei; Huang, Zhi-Yong

    2018-05-01

    A magnetic fluorescent probe of CdTe QDs/nano-Fe3O4@MIPs was prepared using CdTe QDs and Fe3O4 nanoparticles as co-nucleus and molecularly imprinted polymers (MIPs) as specific recognition sites based on a reverse microemulsion method. With the specific enrichment and magnetic separation properties, the probe of CdTe QDs/nano-Fe3O4@MIPs was used to detect malachite green (MG) in fish samples. The TEM analysis showed that the particles of CdTe QDs/nano-Fe3O4@MIPs were spherical with average diameter around 53 nm, and a core-shell structure was well-shaped with several Fe3O4 nanoparticles and CdTe QDs embedded in each of the microsphere. Quick separation of the probes from solutions could be realized with a magnet, indicating the excellent magnetic property of CdTe QDs/nano-Fe3O4@MIPs. The probe exhibited high specific adsorption towards MG and excellent fluorescence emission at λem 598 nm. The fluorescence of CdTe QDs/nano-Fe3O4@MIPs could be linearly quenched by MG at the concentrations from 0.025 to 1.5 μmol L-1. The detection limit was 0.014 μmol L-1. The average recovery of spiked MG in fish samples was 105.2%. The result demonstrated that the as-prepared CdTe QDs/nano-Fe3O4@MIPs could be used as a probe to the detection of trace MG in fish samples.

  5. A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization (FISH

    Directory of Open Access Journals (Sweden)

    Jinzhong FU

    2009-04-01

    Full Text Available The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA. Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number, location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation, low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution.

  6. Chromogenic in situ hybridization (CISH) to detect HER2 gene amplification in breast and gastric cancer: comparison with immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Kiyose, Shinichiro; Igarashi, Hisaki; Nagura, Kiyoko; Kamo, Takaharu; Kawane, Kazunori; Mori, Hiroki; Ozawa, Takachika; Maeda, Matsuyoshi; Konno, Keisuke; Hoshino, Hideaki; Konno, Hiroyuki; Ogura, Hiroyuki; Shinmura, Kazuya; Hattori, Naohiko; Sugimura, Haruhiko

    2012-11-01

    The chromogenic in situ hybridization (CISH) assay, designed to detect the amplification of the HER2 gene in formalin-fixed, paraffin-embedded (FFPE) breast cancer (BC) and gastric cancer (GC) tissue specimens, was evaluated in 125 FFPE BC cases and 198 FFPE GC cases for which the HER2 status had been predetermined using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). In the 125 BC cases and the 198 gastric cases, we found a very good concordance (98.4% and 99.0%, respectively) between CISH and FISH. In particular, we evaluated the polysomy cases, as these cases often have ambiguous treatment options in clinical practice. The polysomy of chromosome 17 was defined as the presence of three or more CEP17 signals in at least 10% of the tumor cells. In the 50 BC cases and 54 GC cases displaying chromosome 17 polysomy, the concordance between FISH and CISH was 98.0% and 98.1%, respectively. These results indicate that CISH could provide an accurate and practical alternative to FISH for the clinical diagnosis of HER2 gene amplification in FFPE BC and FFPE GC samples. © 2012 The Authors. Pathology International © 2012 Japanese Society of Pathology and Wiley Publishing Asia Pty Ltd.

  7. Fluorescence in situ hybridization (FISH screening for the 22q11.2 deletion in patients with clinical features of velocardiofacial syndrome but without cardiac anomalies

    Directory of Open Access Journals (Sweden)

    Paula Sandrin-Garcia

    2007-01-01

    Full Text Available The velocardiofacial syndrome (VCFS, a condition associated with 22q11.2 deletions, is characterized by a typical facies, palatal anomalies, learning disabilities, behavioral disturbances and cardiac defects. We investigated the frequency of these chromosomal deletions in 16 individuals with VCFS features who presented no cardiac anomalies, one of the main characteristics of VCFS. Fluorescent in situ hybridization (FISH with the N25 (D22S75; 22q11.2 probe revealed deletions in ten individuals (62%. Therefore, even in the absence of cardiac anomalies testing for the 22q11.2 microdeletions in individuals showing other clinical features of this syndrome is recommended.

  8. Pharmacological characterization of the oxytocic peptides in the pituitary of a marine teleost fish (pollachius virens) (1961); Caracterisation pharmacologique des peptides ocytociques dans la glande pituitaire d'un poisson marin teleost (pollachius virens) (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Maetz, J; Morel, F [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Heller, H; Pickering, B T [Bristol Univ. (United Kingdom)

    1961-07-01

    Demonstration of the existence, in the neurohypophysis of a marine fish (pollachius virens), of an oxytocic-active peptide different to the oxytocin in mammals and to the arginine vasotocin. (authors) [French] Mise en evidence dans la neurohypophyse d'un poisson marin (pollachius virens) d'un peptide a activite ocytocique different de l'ocytocine des mammiferes et de l'arginine vasotocine. (auteurs)

  9. Pharmacological characterization of the oxytocic peptides in the pituitary of a marine teleost fish (pollachius virens) (1961); Caracterisation pharmacologique des peptides ocytociques dans la glande pituitaire d'un poisson marin teleost (pollachius virens) (1961)

    Energy Technology Data Exchange (ETDEWEB)

    Maetz, J.; Morel, F. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires; Heller, H.; Pickering, B.T. [Bristol Univ. (United Kingdom)

    1961-07-01

    Demonstration of the existence, in the neurohypophysis of a marine fish (pollachius virens), of an oxytocic-active peptide different to the oxytocin in mammals and to the arginine vasotocin. (authors) [French] Mise en evidence dans la neurohypophyse d'un poisson marin (pollachius virens) d'un peptide a activite ocytocique different de l'ocytocine des mammiferes et de l'arginine vasotocine. (auteurs)

  10. [HER-2 oncogene amplification assessment in invasive breast cancer by dual-color in situ hybridization (dc-CISH): a comparative study with fluorescent in situ hybridization (FISH)].

    Science.gov (United States)

    Akhdar, Abbas; Bronsard, Marc; Lemieux, Renald; Geha, Sameh

    2011-12-01

    The amplification of the gene encoding for the human epidermal growth factor receptor 2 (HER-2 oncogene), located on chromosome 17 (17q21-q22), or the overexpression of this receptor have prognostic and therapeutic implications in invasive breast cancer. An evaluation of the HER-2 status by immunohistochemistry (IHC) is performed on all invasive breast cancer cases. Fluorescent in situ hybridization (FISH) is considered as the gold standard for the detection of HER-2 gene amplification for IHC equivocal cases (score 2+). A more recent in situ hybridization technique, the dual-color chromogenic in situ hybridization (dc-CISH), has been proposed as an alternative to FISH. The aim of this study was to measure the correlation between dc-CISH and FISH for HER-2 oncogene amplification assessment in invasive breast cancer. We built four tissue micro-array (TMA) blocs with 100 breast invasive cancer cases that had been previously tested by IHC for HER-2 detection: 10 score 0 cases, 10 score 3+cases, 39 score 1+and 41 score 2+cases. Both FISH and dc-CISH techniques were applied on all TMA cases as well as on two additional slides serving as controls. Interpretation of dc-CISH was carried out by a pathologist using an optical microscope. For FISH, the interpretation was done by a professional from the medical genetics department using a fluorescent microscope linked to a computer system for image capturing and analysis. The interpretation of the HER-2/CEN-17 ratio for both tests was in accordance with the values of the updated recommendations from the Canadian National Consensus Meeting on HER-2/neu testing in breast cancer and from the ASCO/CAP. Among the 100 cases initially included in the study, eight were excluded from the analysis due to sampling or technical flaws. From the 92 remaining cases, we obtained a concordance of 97.8% (90/92 cases) between the two techniques (Kappa coefficient 0.97, 95% confidence interval). The correlation coefficient (rho) between ratios

  11. Extensive polymorphism and chromosomal characteristics of ribosomal DNA in a loach fish, Cobitis vardarensis (Ostariophysi, Cobitidae) detected by different banding techniques and fluorescence in situ hybridization (FISH)

    Czech Academy of Sciences Publication Activity Database

    Rábová, Marie; Ráb, Petr; Ozouf-Costaz, C.

    2001-01-01

    Roč. 111, - (2001), s. 413-422 ISSN 0016-6707 R&D Projects: GA ČR GA206/00/0668; GA AV ČR IAA6045704; GA AV ČR KSK6005114 Keywords : chromosome banding * cytotaxonomy * fish Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.916, year: 2001

  12. Resolving colocalization of bacteria and metal(loid)s on plant root surfaces by combining fluorescence in situ hybridization (FISH) with multiple-energy micro-focused X-ray fluorescence (ME μXRF).

    Science.gov (United States)

    Honeker, Linnea K; Root, Robert A; Chorover, Jon; Maier, Raina M

    2016-12-01

    Metal(loid)-contamination of the environment due to anthropogenic activities is a global problem. Understanding the fate of contaminants requires elucidation of biotic and abiotic factors that influence metal(loid) speciation from molecular to field scales. Improved methods are needed to assess micro-scale processes, such as those occurring at biogeochemical interfaces between plant tissues, microbial cells, and metal(loid)s. Here we present an advanced method that combines fluorescence in situ hybridization (FISH) with synchrotron-based multiple-energy micro-focused X-ray fluorescence microprobe imaging (ME μXRF) to examine colocalization of bacteria and metal(loid)s on root surfaces of plants used to phytostabilize metalliferous mine tailings. Bacteria were visualized on a small root section using SytoBC nucleic acid stain and FISH probes targeting the domain Bacteria and a specific group (Alphaproteobacteria, Gammaproteobacteria, or Actinobacteria). The same root region was then analyzed for elemental distribution and metal(loid) speciation of As and Fe using ME μXRF. The FISH and ME μXRF images were aligned using ImageJ software to correlate microbiological and geochemical results. Results from quantitative analysis of colocalization show a significantly higher fraction of As colocalized with Fe-oxide plaques on the root surfaces (fraction of overlap 0.49±0.19) than to bacteria (0.072±0.052) (proots, metal(loid)s and microbes, information that should lead to improved mechanistic models of metal(loid) speciation and fate. Copyright © 2016 Elsevier B.V. All rights reserved.

  13. Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA-FISH).

    Science.gov (United States)

    Kubota, Kengo; Ohashi, Akiyoshi; Imachi, Hiroyuki; Harada, Hideki

    2006-09-01

    Two-pass tyramide signal amplification-fluorescence in situ hybridization (two-pass TSA-FISH) with a horseradish peroxidase (HRP)-labeled oligonucleotide probe was applied to detect prokaryotic mRNA. In this study, mRNA of a key enzyme for methanogenesis, methyl coenzyme M reductase (mcr), in Methanococcus vannielii was targeted. Applicability of mRNA-targeted probes to in situ hybridization was verified by Clone-FISH. It was observed that sensitivity of two-pass TSA-FISH was significantly higher than that of TSA-FISH, which was further increased by the addition of dextran sulphate in TSA working solution. Signals from two-pass TSA-FISH were more reliable compared to the weak, spotty signals yielded by TSA-FISH.

  14. DAMPAK PEMBUANGAN LIMBAH TERHADAP PERUBAHAN KUALITAS OSEANOGRAFI BIOFISIK-KIMIA DAN PRODUKSI IKAN TERI (Stolephorus spp. DI PERAIRAN LAUT TELUK AMBON (The Impact of Waste Disposal on the Biophysical-chemical Characteristics Changes and Teri fish

    Directory of Open Access Journals (Sweden)

    Latif Sahubawa

    2001-04-01

    m2 pada musim Barat di wilayah Ambang Galala-Rumahtiga. Potensi ikan pelagis kecil pada musim Timur 63.968,76 tor/tahun, Peralihan 56.311,55 ton/tahun, dan Barat 60.244,35 ton/tahun atau 3,86Vo dari total potensi ikan pelagis kecil perairan Maluku (1.564,000 ton/tahun. Densitas ikan pelagis kecit pab musim Timur 34,62 kg/m3, Peralihan 29,83 kg/m3, dan Barat 32,33 kg/m3. Tingkat eksploitasi sumber daya ikan pelagis kecil perairan Teluk Ambon yaitu 30 % ("status sedang berkembang".   ABSTRACT The research objectives are: (1 identify the characteristics of waste from human activities that cause reduced biophysical-chemical oceanography characteristics of Ambon Bay Marine; (2 to evaluate the reduced biophysichemical oceanography characteristics of Ambon Bay marine in relation to assigmen requirements for fish aquaticulture; and (3 to evaluate the effect of assignment requirements fulfillment on the abundance and density of small pelagic fish, and teri fish production during Eastern and Western monsoons. Research samples consisted of sea water, teri fish, and mollusca. Sample collection methods were simple random and nonrandom sampling. Data were analyzed by using Norman Curve, Least Square, Factorial and Block completely Randomized Design with F-test, Nutritional Value Coefficient (NVC, surplus production, Hydroacoustic and Utermohl Sedimentation Methods. The results of the statistical analyses show that in general, the variables did not affect the biophysical-chemical oceanographic parameters of the Ambon Bay marine waters. The values range of the biophysical-chemical oceanography parameters of Ambon Bay marine are: temperature 23.7-28oC, TSS 2.005-12.436 mg/l, salinity 24.00-32.66 ppt, clearance 2.5-9.0 meters, pH 6.5-8.6, solved oxygen 2.09-6.88 mg/l, BOD5 10.0-50.0 mg/l, COD 22.5-150.8 mg/l, PO43- 0.22-329 µg/l, NO2- 0.22-2.94 µg/l, NO3- 0.21-15.40 µg/l, Hg in the water body 0.001-0.065 mg/l and mollusca meat 0.115-0.741 mg/l, and hydrocarbons 0.011-2.540 mg

  15. Evaluation of gene amplification and protein expression of HER-2/neu in esophageal squamous cell carcinoma using Fluorescence in situ Hybridization (FISH) and immunohistochemistry

    International Nuclear Information System (INIS)

    Sato-Kuwabara, Yukie; Neves, José I; Fregnani, José HTG; Sallum, Rubens A; Soares, Fernando A

    2009-01-01

    Esophageal squamous cell carcinoma (ESCC) is the sixth most frequent neoplasia in Brazil. It is usually associated with a poor prognosis because it is often at an advanced stage when diagnosed and there is a high frequency of lymph node metastases. It is important to know what prognostic factors can facilitate diagnosis, optimize therapeutic decisions, and improve the survival of these patients. A member of the epidermal growth factor receptor (EGFR) family, c-erbB-2, has received much attention because of its therapeutic implications; however, few studies involving fluorescence in situ hybridization (FISH) analysis of HER-2/neu gene amplification and protein expression in ESCC have been conducted. The aim of this study was to verify the presence of HER-2/neu gene amplification using FISH, and to correlate the results with immunohistochemical expression and clinical-pathological findings. One hundred and ninety-nine ESCC cases were evaluated using the Tissue Microarray (TMA) technique. A polyclonal antibody against c-erbB-2 was used for immunohistochemistry. Analyses were based on the membrane staining pattern. The results were classified according to the Herceptest criteria (DAKO): negative (0/1+), potential positive (2+) and positive (3+). The FISH reactions were performed according to the FISH HER2 PharmDx (DAKO) protocol. In each case, 100 tumor nuclei were evaluated. Cases showing a gene/CEN17 fluorescence ratio ≥ 2 were considered positive for gene amplification. The c-erbB-2 expression was negative in 117/185 cases (63.2%) and positive in 68 (36.8%), of which 56 (30.3%) were 2+ and 12 (6.5%) were 3+. No significant associations were found among protein expression, clinicopathological data and overall survival. Among the 47 cases analyzed, 38 (80.9%) showed no gene amplification while 9 (19.1%) showed amplification, as demonstrated by FISH. Cases that were negative (0/1+) and potential positive (2+) for c-erbB-2 expression by immunohistochemistry showed no

  16. Fluorescence In Situ Hybridization (FISH-Based Karyotyping Reveals Rapid Evolution of Centromeric and Subtelomeric Repeats in Common Bean (Phaseolus vulgaris and Relatives

    Directory of Open Access Journals (Sweden)

    Aiko Iwata-Otsubo

    2016-04-01

    Full Text Available Fluorescence in situ hybridization (FISH-based karyotyping is a powerful cytogenetics tool to study chromosome organization, behavior, and chromosome evolution. Here, we developed a FISH-based karyotyping system using a probe mixture comprised of centromeric and subtelomeric satellite repeats, 5S rDNA, and chromosome-specific BAC clones in common bean, which enables one to unambiguously distinguish all 11 chromosome pairs. Furthermore, we applied the karyotyping system to several wild relatives and landraces of common bean from two distinct gene pools, as well as other related Phaseolus species, to investigate repeat evolution in the genus Phaseolus. Comparison of karyotype maps within common bean indicates that chromosomal distribution of the centromeric and subtelomeric satellite repeats is stable, whereas the copy number of the repeats was variable, indicating rapid amplification/reduction of the repeats in specific genomic regions. In Phaseolus species that diverged approximately 2–4 million yr ago, copy numbers of centromeric repeats were largely reduced or diverged, and chromosomal distributions have changed, suggesting rapid evolution of centromeric repeats. We also detected variation in the distribution pattern of subtelomeric repeats in Phaseolus species. The FISH-based karyotyping system revealed that satellite repeats are actively and rapidly evolving, forming genomic features unique to individual common bean accessions and Phaseolus species.

  17. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH).

    Science.gov (United States)

    Cortés-Gutiérrez, Elva I; Ortíz-Hernández, Brenda L; Dávila-Rodríguez, Martha I; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

    2013-02-19

    We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  18. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH

    Directory of Open Access Journals (Sweden)

    Jaime Gosálvez

    2013-02-01

    Full Text Available We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH. A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL, 10 with high-grade SIL (HG-SIL, and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

  19. Utility and impact of early t(15;17) identification by Fluorescence In Situ Hybridization (FISH) in clinical decision making for patients in Acute Promyelocytic Leukemia (APL).

    Science.gov (United States)

    Kolhe, R; Mangaonkar, A; Mansour, J; Clemmons, A; Shaw, J; Dupont, B; Walczak, L; Mondal, A; Rojiani, A; Jillella, A; Kota, V

    2015-08-01

    Acute Promyelocytic Leukemia (APL) is a curable malignancy with studies showing above 90% survival. However, population-based studies looking at survival suggest that approximately 30% of patients with APL die during induction. Early demonstration of t(15;17) will lead to accurate decision making regarding treatment. The aim of this project was to validate earlier time frames for the Abbott Molecular Vysis LSI promyelocytic leukemia (PML)/ retinoic acid receptor alpha (RARA) fluorescence in situ hybridization (FISH) probe (ASR 6-16 h). Twenty patients (15 APL cases and five non-APL cases) were selected for validating various hybridization times for the FISH probe. Expected normal signal pattern was two red and two green signals (2R2G), and the most common expected abnormal signal pattern was two fusion (yellow) signals, one red and one green (2F1R1G) and/or one fusion, one red and one green (1F1R1G). The specificity of the probe ranged from 84% at 2 h, 86% at 4 h, 84% at 6 h, and 87% for overnight hybridization. The sensitivity increased from 79% at 2 h, 80% at 4 h, 81% at 6 h to 87% for overnight hybridization. Based on the validation studies, we recommend reading of FISH results at the 4-h incubation mark for a preliminary diagnosis and confirmation with overnight hybridization. © 2015 John Wiley & Sons Ltd.

  20. HER2 Gene Amplification Testing by Fluorescent In Situ Hybridization (FISH): Comparison of the ASCO-College of American Pathologists Guidelines With FISH Scores Used for Enrollment in Breast Cancer International Research Group Clinical Trials.

    Science.gov (United States)

    Press, Michael F; Sauter, Guido; Buyse, Marc; Fourmanoir, Hélène; Quinaux, Emmanuel; Tsao-Wei, Denice D; Eiermann, Wolfgang; Robert, Nicholas; Pienkowski, Tadeusz; Crown, John; Martin, Miguel; Valero, Vicente; Mackey, John R; Bee, Valerie; Ma, Yanling; Villalobos, Ivonne; Campeau, Anaamika; Mirlacher, Martina; Lindsay, Mary-Ann; Slamon, Dennis J

    2016-10-10

    Purpose ASCO and the College of American Pathologists (ASCO-CAP) recently recommended further changes to the evaluation of human epidermal growth factor receptor 2 gene (HER2) amplification by fluorescent in situ hybridization (FISH). We retrospectively assessed the impact of these new guidelines by using annotated Breast Cancer International Research Group (BCIRG) -005, BCIRG-006, and BCIRG-007 clinical trials data for which we have detailed outcomes. Patients and Methods The HER2 FISH status of BCIRG-005/006/007 patients with breast cancers was re-evaluated according to current ASCO-CAP guidelines, which designates five different groups according to HER2 FISH ratio and average HER2 gene copy number per tumor cell: group 1 (in situ hybridization [ISH]-positive): HER2-to-chromosome 17 centromere ratio ≥ 2.0, average HER2 copies ≥ 4.0; group 2 (ISH-positive): ratio ≥ 2.0, copies < 4.0; group 3 (ISH-positive): ratio < 2.0, copies ≥ 6.0; group 4 (ISH-equivocal): ratio < 2.0, copies ≥ 4.0 and < 6.0; and group 5 (ISH-negative): ratio < 2.0, copies < 4.0. We assessed correlations with HER2 protein, clinical outcomes by disease-free survival (DFS) and overall survival (OS) and benefit from trastuzumab therapy (hazard ratio [HR]). Results Among 10,468 patients with breast cancers who were successfully screened for trial entry, 40.8% were in ASCO-CAP ISH group 1, 0.7% in group 2; 0.5% in group 3, 4.1% in group 4, and 53.9% in group 5. Distributions were similar in screened compared with accrued subpopulations. Among accrued patients, FISH group 1 breast cancers were strongly correlated with immunohistochemistry 3+ status (P < .0001), whereas groups 2, 3, 4, and 5 were not; however, groups 2, 4 and, 5 were strongly correlated with immunohistochemistry 0/1+ status (all P < .0001), whereas group 3 was not. Among patients accrued to BCIRG-005, group 4 was not associated with significantly worse DFS or OS compared with group 5. Among patients accrued to BCIRG-006, only

  1. Specific Fluorescence in Situ Hybridization (FISH) Test to Highlight Colonization of Xylem Vessels by Xylella fastidiosa in Naturally Infected Olive Trees (Olea europaea L.)

    Science.gov (United States)

    Cardinale, Massimiliano; Luvisi, Andrea; Meyer, Joana B.; Sabella, Erika; De Bellis, Luigi; Cruz, Albert C.; Ampatzidis, Yiannis; Cherubini, Paolo

    2018-01-01

    The colonization behavior of the Xylella fastidiosa strain CoDiRO, the causal agent of olive quick decline syndrome (OQDS), within the xylem of Olea europaea L. is still quite controversial. As previous literature suggests, even if xylem vessel occlusions in naturally infected olive plants were observed, cell aggregation in the formation of occlusions had a minimal role. This observation left some open questions about the whole behavior of the CoDiRO strain and its actual role in OQDS pathogenesis. In order to evaluate the extent of bacterial infection in olive trees and the role of bacterial aggregates in vessel occlusions, we tested a specific fluorescence in situ hybridization (FISH) probe (KO 210) for X. fastidiosa and quantified the level of infection and vessel occlusion in both petioles and branches of naturally infected and non-infected olive trees. All symptomatic petioles showed colonization by X. fastidiosa, especially in the larger innermost vessels. In several cases, the vessels appeared completely occluded by a biofilm containing bacterial cells and extracellular matrix and the frequent colonization of adjacent vessels suggested a horizontal movement of the bacteria. Infected symptomatic trees had 21.6 ± 10.7% of petiole vessels colonized by the pathogen, indicating an irregular distribution in olive tree xylem. Thus, our observations point out the primary role of the pathogen in olive vessel occlusions. Furthermore, our findings indicate that the KO 210 FISH probe is suitable for the specific detection of X. fastidiosa. PMID:29681910

  2. Mercury speciation in thawed out and refrozen fish samples by gas chromatography coupled to inductively coupled plasma mass spectrometry and atomic fluorescence spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Krystek, Petra; Ritsema, Rob [National Institute for Public Health and the Environment (RIVM), Laboratory for Analytical Chemistry, Bilthoven (Netherlands)

    2005-01-01

    Different sub-sampling procedures were applied for the determination of mercury species (as total mercury Hg, methylmercury MeHg{sup +} and inorganic mercury Hg{sup 2+}) in frozen fish meat. Analyses were carried out by two different techniques. After the sample material was pre-treated by microwave digestion, atomic fluorescence spectroscopy (AFS) was used for the determination of total Hg. Speciation analysis was performed according to the following procedure: dissolution of sample material in tetramethylammonium hydroxide (TMAH), derivatisation with sodium tetraethylborate (NaBEt{sub 4}), extraction into isooctane and measurement with gas chromatography inductively coupled plasma mass spectrometry (GC-ICPMS) for the identification and quantification of methylmercury (MeHg{sup +}) and inorganic mercury (Hg{sup 2+}). The concentration range of total Hg measured in the shark fillets is between 0.9 and 3.6 {mu}g g{sup -1} thawed out shark fillet. Speciation analysis leads to {>=}94% Hg present as MeHg{sup +}. Homogeneity, storage conditions and stability of analytical species and sample materials have great influence on analytical results. Sub-sampling of half-frozen/partly thawed out fish and analysis lead to significantly different concentrations, which are on average a factor of two lower. (orig.)

  3. Considerations in the use of fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy to characterize rumen methanogens and define their spatial distributions.

    Science.gov (United States)

    Valle, Edith R; Henderson, Gemma; Janssen, Peter H; Cox, Faith; Alexander, Trevor W; McAllister, Tim A

    2015-06-01

    In this study, methanogen-specific coenzyme F420 autofluorescence and confocal laser scanning microscopy were used to identify rumen methanogens and define their spatial distribution in free-living, biofilm-, and protozoa-associated microenvironments. Fluorescence in situ hybridization (FISH) with temperature-controlled hybridization was used in an attempt to describe methanogen diversity. A heat pretreatment (65 °C, 1 h) was found to be a noninvasive method to increase probe access to methanogen RNA targets. Despite efforts to optimize FISH, 16S rRNA methanogen-specific probes, including Arch915, bound to some cells that lacked F420, possibly identifying uncharacterized Methanomassiliicoccales or reflecting nonspecific binding to other members of the rumen bacterial community. A probe targeting RNA from the methanogenesis-specific methyl coenzyme M reductase (mcr) gene was shown to detect cultured Methanosarcina cells with signal intensities comparable to those of 16S rRNA probes. However, the probe failed to hybridize with the majority of F420-emitting rumen methanogens, possibly because of differences in cell wall permeability among methanogen species. Methanogens were shown to integrate into microbial biofilms and to exist as ecto- and endosymbionts with rumen protozoa. Characterizing rumen methanogens and defining their spatial distribution may provide insight into mitigation strategies for ruminal methanogenesis.

  4. Phenotypic consequences of a mosaic marker chromosome identified by fluorescence in situ hybridization (FISH) as being derived from chromosome 16

    Energy Technology Data Exchange (ETDEWEB)

    Ray, J.H.; Zhou, X.; Pletcher, B.A. [Cornell Univ. Medical College, Manhasset, NY (United States)] [and others

    1994-09-01

    De novo marker chromosomes are detected in 1 in 2500 amniotic fluid samples and are associated with a 10-15% risk for phenotypic abnormality. FISH can be utilized as a research tool to identify the origins of marker chromosomes. The phenotypic consequences of a marker chromosome derived from the short arm of chromosome 16 are described. A 26-year-old woman underwent amniocentesis at 28 weeks gestation because of a prenatally diagnosed tetralogy of Fallot. Follow-up ultrasounds also showed ventriculomegaly and cleft lip and palate. 32 of 45 cells had the karyotype 47,XY,+mar; the remaining cells were 46,XY. The de novo marker chromosome was C-band positive and non-satellited and failed to stain with distamycin A/DAPI. At birth the ultrasound findings were confirmed and dysmorphic features and cryptorchidism were noted. Although a newborn blood sample contained only normal cells, mosaicism was confirmed in 2 skin biopsies. FISH using whole-chromosome painting and alpha-satellite DNA probes showed that the marker chromosome had originated from chromosome 16. As proximal 16q is distamycin A/DAPI positive, the marker is apparently derived from proximal 16p. At 15 months of age, this child is hypotonic, globally delayed and is gavage-fed. His physical examination is significant for microbrachycephaly, a round face, sparse scalp hair, ocular hypertelorism, exotropia, a flat, wide nasal bridge and tip, mild micrognathia, and tapered fingers with lymphedema of hands and feet. Inguinal hernias have been repaired. His features are consistent with those described for patients trisomic for most or all of the short arm of chromosome 16. Marker chromosomes derived from the short arm of chromosome 16 appear to have phenotypic consequences. As the origin of more marker chromosomes are identified using FISH, their karyotype/phenotype correlations will become more apparent, which will permit more accurate genetic counseling.

  5. KONSUMSI IKAN LAUT KADAR MERCURY DALAM RAMBUT DAN KESEHATAN NELAYAN DI PANTAI KENJERAN SURABAYA (Sea Fish Consumption, Degree of Mercury Content in Hair, and Fisherman Health at Surabaya Kenjeran Beach, Indonesia

    Directory of Open Access Journals (Sweden)

    Sudarmaji Sudarmaji

    2004-11-01

    Full Text Available ABSTRAK Pantai Kenjeran di Surabaya mempunyai banyak fungsi baik sebagai tempat rekreasi, perikanan serta tempat pembuangan limbah dari kota Surabaya. Studi sebelumnya telah menjelaskan bahwa pantai Kenjeran telah tercemar khususnya Hg. Polutan ini telah diindikasikan terdapat dalam ikan yang dikonsumsi masyarakat sekitar. Penelitian ini mengkaji dampak mengkonsumsi ikan dari Kenjeran kaitannya dengan kesehatan masyarakat yang menkonsumsi ikan. Peneliltian ini rnengambil sample 70 orang yang mengkonsumsi ikan dan 45 orang sebagai kontrol grup. Dalarn penelitian ini rambut responden diambil dan dikaji dengan Atomic Absorption Spectrophotometer (AAS. Penelitian ini menghasilkan bahwa responden yang mengkonsumsi ikan sebanyak rata-rata 99,11 gram/hari mempunyai kadar Hg dalam rambutnya sebesar 0.511 ppb. Penelitian ini mengindikasikan gejala-gejala penyakit yang terjadi pada mereka yang rnengkonsumsi ikan antara lain ginjal, pusing-pusing, tumor, pendarahan gusi, dan gangguan penglihatan. Penelitian ini rnenyimpulkan adanya korelasi yang signifikan antara responden yang mengkonsumsi ikan yang tercemar dengan kadar Hg dalam rambutnya.   ABSTRACT Surabaya Kenjeran Beach, as a part of Eastern coastal area at East Java, functions as a sea recreation place and fishing. The condition of Surabaya Kenderan Beach is polluted by Hg as observed by previous researchers. They suggested that water, sediment, and fish from Kenjeran beach were already contaminated by Hg at dangerous level. Fisherman communities is one of the group which have a risk of getting affected by methyl Hg, because they usually consume fish from sea. This research is to study the relationship between consumption of sea fish and degree of Hg in fisherman’s hair, to measure the average degree of Hg in their hair and then to compare it with limit value. It is also studying the health disorder that likely appears as a result of Hg poisoning. This research took place at Kenjeran district

  6. Large-scale polymorphism near the ends of several human chromosomes analyzed by using fluorescence in situ hybridization (FISH)

    Energy Technology Data Exchange (ETDEWEB)

    Trask, B.J.; Friedman, C. [Univ. of Washington, Seattle, WA (United States); Giorgi, D. [CNRS, Montpelier (France)] [and others

    1994-09-01

    We have discovered a large DNA segment that is polymorphically present at the ends of several human chromosomes. The segment, f7501, was originally derived form a human chromosome 19-specific cosmid library. FISH was used to determine the cosmid`s chromosomal distribution on 44 unrelated humans and several closely related primates. The human subjects represent a diversity of reproductively isolated ethnic populations. FISH analysis revealed that sequences highly homologous to the cosmid`s insert are present on both homologs at 3q, 15q,. and 19p in almost all individuals (88, 85, and 87 of 88 homologs, respectively). Other chromosomes sites were labeled much more rarely in the sampled individuals. For example, 56 of the 88 analyzed chromosomes 11 were labeled (18+/+, 6-/-, and 20+/- individuals). In contrast, 2q was labeled on only 1/88 sampled chromosomes. The termini of 2q, 5q, 6p, 6q, 7p, 8p, 9p, 9q, 11p, 12q, 16p, 19q, and 20q and an interstitial site at 2q13-14 were labeled in at least one individual of the set. EcoR1-fragments derived from the cosmid showed the same hybridization pattern as the entire cosmid, indicating that at least 40 kbp is shared by these chromosome ends. Ethnic differences in the allele frequency of these polymorphic variants was observed. For example, signals were observed on 8/10 and 7/10 of the chromosomes 7p and 16q, respectively, derived form Biakan Pygmies, but these sites were infrequently labeled in non-Pygmy human populations (2/68, respectively). This region has undergone significant changes in chromosome location during human evolution. Strong signal was seen on chimpanzee and gorilla chromosome 3, which is homologous to human chromosome 4, a chromosome unlabeled in any of the humans we have analyzed.

  7. Ultra-high performance liquid chromatography with fluorescence detection following salting-out assisted liquid-liquid extraction for the analysis of benzimidazole residues in farm fish samples.

    Science.gov (United States)

    Tejada-Casado, Carmen; Lara, Francisco J; García-Campaña, Ana M; Del Olmo-Iruela, Monsalud

    2018-03-30

    Ultra-high performance liquid chromatography (UHPLC) coupled with fluorescence detection (FL) has been proposed for the first time to determine thirteen benzimidazoles (BZs) in farmed fish samples. In order to optimize the chromatographic separation, parameters such as mobile phase composition and flow rate were carefully studied, establishing a gradient mode with a mobile phase consisted of water (solvent A) and acetonitrile (solvent B) at a flow rate of 0.4 mL/min. The separation was performed on a Zorbax Eclipse Plus RRHD C 18 column (50 × 2.1 mm, 1.8 μm), involving a total analysis time lower than 12 min. Salting-out assisted liquid-liquid extraction (SALLE) was applied as sample treatment to different types of farmed fish (trout, sea bream and sea bass). To obtain satisfactory extraction efficiencies for the studied analytes, several parameters affecting the SALLE procedure were optimized including the amount of sample, type and volume of the extraction solvent, and the nature and amount of the salt used. Characterization of the method in terms of performance characteristics was carried out, obtaining satisfactory results for the linearity (R 2  ≥ 0.997), repeatability (RSD ≤ 6.1%), reproducibility (RSD ≤ 10.8%) and recoveries (R ≥ 79%; RSD ≤ 7.8%). Detection limits between 0.04-29.9 μg kg -1 were obtained, demonstrating the applicability of this fast, simple and environmentally friendly method. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. Human chromosome-specific changes in a human-hamster hybrid cell line (AL) assessed by fluorescent in situ hybridization (fish)

    International Nuclear Information System (INIS)

    Geard, Charles R.; Jenkins, Gloria

    1995-01-01

    Purpose: To quantitatively assess all gamma-ray induced chromosomal changes confined to one human chromosome using fluorescence microscopy and in situ hybridization with a fluorescently labeled human chromosome specific nucleic acid probe. Methods and Materials: Synchronized human-hamster hybrid cells containing human chromosome 11 were obtained by a modified mitotic shake-off procedure. G1 phase cells (> 95%) were irradiated with 137 Cs gamma rays (0, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, and 10.0 Gy) at a dose rate of 1.1 Gy/min and mitotic cells collected 16-20 h later; chromosomal spreads were prepared, denatured, and hybridized with a fluorescein-tagged nucleic acid probe against total human DNA. Chromosomes were examined by fluorescence microscopy and all categories of change involving the human chromosome 11 as target, recorded. Results: Overall, of the 3104 human-hamster hybrid cells examined, 82.1% were euploid, of which 88.6% contained one copy of human chromosome 11, 6.2% contained two copies, and 5.2% contained 0 copies. This is compatible with mitotic nondisjunction in a small fraction of cells. Of the remaining 17.9% of cells, 85.2% were tetraploid cells with two copies of human chromosome 11. For all aberrations involving human chromosome 11 there was a linear relationship between yield and absorbed dose of 0.1 aberrations per chromosome per Gy. The yield of dicentrics, translocations, and terminal deletions that involve one lesion on the human chromosome was linear, while the yield of interstitial deletions that arise from two interacting lesions on the human chromosome was curvilinear. The frequencies of dicentrics and translocations were about equal, while there was a high (40-60%) incidence of incomplete exchanges between human and hamster chromosomes. Conclusions: Fluorescent in situ hybridization (FISH) procedures allow for the efficient detection of a broad range of induced changes in target chromosomes. Symmetrical exchanges induced in G1

  9. Fluorescent ligand fishing combination with in-situ imaging and characterizing to screen Hsp 90 inhibitors from Curcuma longa L. based on InP/ZnS quantum dots embedded mesoporous nanoparticles.

    Science.gov (United States)

    Hu, Yue; Fu, Anchen; Miao, Zhaoyi; Zhang, Xiaojing; Wang, Tianlin; Kang, An; Shan, Jinjun; Zhu, Dong; Li, Wei

    2018-02-01

    Although ligand fishing has been shown to be an efficient technique for the identification of bioactive components from complex mixtures such as natural products, it cannot be applied to biomedical image processing. Herein, a specific fluorescent ligand fishing combined with in situ imaging approach is presented for the identification of heat shock protein 90 (Hsp 90) inhibitors from complex matrixes, Curcuma longa L., using N-terminus immobilized Hsp 90α functionalized InP/ZnS quantum dots embedded mesoporous nanoparticles (i.e. Hsp 90α (NT)-FQDNs) as extraction sorbents and fluorescent tracer. The fished ligands were identified by liquid chromatography time-of-flight/mass spectrometry (LC-TOF/MS) and gas chromatography-mass spectrometry (GC-MS). Moreover, in situ imaging by confocal laser scanning microscopy (CLSM) was applied for evaluating the effect of fished-ligands on bioactivity-induced apoptosis morphologically in HeLa cells. MTT assay verified the bioactivity of the ligands and molecular docking results further provided convincing information to verify the feasible binding mode between ligands and protein. Twelve ligands as potential Hsp 90 inhibitors were ultimately fished and identified from Curcuma longa L. crude extracts. The proposed approach based on Hsp 90α functionalized nanocomposites is superior in the combination of highly specific screening efficiency and concurrent visual in situ imaging, which could have great promise for the development of other plant-derived Hsp 90 inhibitors, and providing a rapid and reliable platform for discovering biologically active molecules in natural products. Copyright © 2017 Elsevier B.V. All rights reserved.

  10. ANALISIS DAN PREDIKSI BEBAN PENCEMARAN LIMBAH CAIR PABRIK PENGALENGAN IKAN (Analysis and Prediction of Liquid Waste Pollutant Level in Fish Canning Plant

    Directory of Open Access Journals (Sweden)

    Latif Sahubawa

    2011-03-01

    Full Text Available ABSTRAK Penelitian bertujuan untuk mengetahui kadar parameter pencemaran serta beban pencemaran limbah cair pabrik pengalengan ikan sardin. Manfaatnya adalah sebagai saran pertimbangan kepada industri untuk mengelola limbah cair secara profesional, menjamin kelestarian dan peruntukkan badan air penerima limbah cair, serta bahan informasi ilmiah kepada pengambil kebijakan (terutama pejabat daerah dalam pengelolaan kualitas lingkungan hidup secara berkelanjutan. Metode analisis yang digunakan adalah neraca masa, dengan parameter pengamatan yaitu: debit limbah cair maksimum (DM dan debit limbah cair sebenarnya (DA, serta beban pencemaran maksimum (BPM dan beban pencemaran sebenarnya (BPA dari parameter pH, TSS, BOD, COD, dan minyak/lemak sesuai Kepmen LH. No. 06 Tahun 2007. Berdasarkan hasil pengukuran, tercatat debit air limbah aktual (Dp = 10,0 liter/detik, debit air limbah sebenarnya (DA = 2.880 m 3, serta debit air limbah maksimum (DM = 720 m 3, (jadi DA > DM. Dari hasil analisis laboratorium, kadar parameter indikator pencemaran limbah cair pabrik ikan kaleng, masing-masing: pH = 6,5; TSS = 250 mg/l; BOD 5 = 95,0 mg/l; COD = 105 mg/l; dan minyak/lemak = 0,5 mg/l. Berdasarkan hasil perhitungan, ternyata beban pencemaran sebenarnya dari masing-masing parameter (BPA-TSS, BOD 5 , dan COD lebih besar dari beban pencemaran maksimum (BPM, kecuali lemak di mana BPA MWD. Based on the laboratory analytical result, wastewater pollution indicator parameters are, respectively, pH=6.5; TSS=250 mg/l; BOD­5=95.0 mg/l; COD=105 mg/l; and oil/fat = 0.5 mg/l. Based on the measuring result, the substantive pollution load, respectively, higher than maximum pollution load (SPL>MPL, except fat parameter, while SPL less than MPL.  We can conclude that wastewater pollution load produced by canning industries, exceeded the allowed limit of wastewater quality for fisheries industries (Kepmen LH No. 06 Year 2007, and also resulted pollution (changing the utilization in

  11. Use of X-ray fluorescence in a laboratory for the treatment of uranium ores (1960); Utilisation de la fluorescence X dans un laboratoire de traitements de minerais d'uranium (1960)

    Energy Technology Data Exchange (ETDEWEB)

    Guillet, H [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1960-07-01

    A brief description will be given of some aspects of the experience gained over a year during which X-ray fluorescence was used at the laboratory or the present Section Autonome d'Etudes, Recherches et Applications Chimiques of the Commissariat a l'Energie Atomique. First part. - Reproducibility of results. A standard is tested daily. The observations made during the months from december 58 to may 59 are described. Second part. - Study of two chemical treatment processes using X-ray fluorescence, without development of a detailed method of analysis. a) Acid lixiviation of uranium ores. The residues are analysed by X-Ray fluorescence directly in powder form. b) Fixation and elution of vanadium on ion exchange resin. Third part. - Method for the quantitative analysis of the uranium in solution. A method of analysis of the uranium in solution is described for concentrations between 30 {gamma}/cc and 600 {gamma}/cc, whatever may be the impurities present (except for the elements between Zn and Rb, and between Ir and Pa). (author) [French] On se propose de decrire brievement quelques aspects de l'experience acquise en une annee d'utilisation de fluorescence X au laboratoire de l'actuelle Section Autonome d'Etudes, Recherches et Applications chimiques du Commissariat a l'Energie Atomique. Premiere Partie. - Etude de la reproductibilite des resultats. Un standard est teste quotidiennement. On donne la description des observations faites durant les mois de decembre 58 a mai 59. Deuxieme Partie. - Etude de deux procedes de traitements chimiques au moyen de la fluorescence X sans mise au point de methode d'analyse elaboree. a) Liziviation acide de minerais d'uranium. Les residus sont analyses par fluorescence X directement sous forme pulverulente. b) Fixation et elution du vanadium sur resine echangeuse d'ions. Troisieme partie. - Methode d'analyse quantitative de l'uranium en solution. On decrit une methode d'analyse de l'uranium en solution pour des concentrations allant

  12. Caractérisation de l'état chimique d'un élément par fluorescence X : l'aluminium dans les zéolithes Characterizing the Chemical State of an Element by X-Ray Fluorescence: Aluminium in Zeolites

    Directory of Open Access Journals (Sweden)

    Freund E.

    2006-11-01

    Full Text Available La fluorescence X est essentiellement connue dans le domaine de l'analyse quantitative, mais c'est aussi une méthode qui permet d'obtenir des informations détaillées sur la structure électronique des éléments. Dans cet article, nous montrons qu'il est possible de mettre au point une technique simple de caractérisation de l'état chimique d'un élément en utilisant de façon conjointe un spectromètre commercial et un programme adapté de traitement des données. Nous avons appliqué cette technique au cas de l'aluminium dans une zéolithe particulière : la mordénite dite à petits pores modifiée par différents traitements. Nous avons ainsi pu classer les solides suivant leur rapport (AI hexa-coordinné/AI tétracoordinné et montrer que le débouchage de la mordénite dite à petits pores est lié à l'extraction d'atomes d'aluminium de la charpente cristalline. X-ray fluorescence is mainly known in the field of qua-titative analysis, but it is also a useful method for obtaining detailed informations about the electronic structure of elements. This article shows that it is possible to develop a simple technique for characterizing the chemical state of an element by combining a commercial spectrometer with a program suited for data processing. This technique has been applied to the case of aluminum in a specific zeolite, ( small portmodernite, modified by various treatments. We were thus able to classify the solids according to the ratio of hexacoordinated AI/tetracoordinated AI and to show that the deblocking of the small portmordenite is linked to the extraction of aluminum atomrs from the crystalline structure.

  13. PERUBAHAN KARAKTERISTIK SURIMI IKAN CUCUT DAN IKAN PARI AKIBAT PENGARUH PENGKOMPOSISIAN DAN PENYIMPANAN DINGIN DAGING LUMAT [Characteristic Changes of Shark and Stingray Surimi as Affected by Compositioning and Chill Storage of the Mince Fish

    Directory of Open Access Journals (Sweden)

    Joko Santoso1

    2008-06-01

    Full Text Available This experiment was carried out to study the effects of leaching, compositioning, and chill storage of mince fish on the characteristic changes of surimi from shark and stingray fish. Three times leaching of mince fish could reduce the urea contents of the shark and stingray as much as 88% and 100%, respectively; with the salt soluble protein contents were 13.52% and 13.24%, respectively. Mixture of mince shark and stingray in proportion of 25% : 75% (A1B2 gave the highest value of gel strength being 209.29 g.cm in comparison with others composition. During chill storage, deterioration process still occurred as indicated by increasing value of acidity and contents of base volatile compounds i.e. total volatile base nitrogen (TVBN and tri-methyl amine (TMA; and also decreasing contents of urea and salt soluble protein. Deterioration process of mince fish also affected the physical characteristic of surimi i.e. decreasing values of gel strength, water holding capacity (WHC, and colour (whiteness.

  14. Implementation and importance of fluorescence in situ hybridization (fish) in paraffin tissues for categorization of B-cell lymphoma unclassifiable, with features intermediate between Burkitt lymphoma and diffuse large B-cell lymphoma

    International Nuclear Information System (INIS)

    Carvajal Cuenca, Alejandra

    2011-01-01

    The diagnostic criteria have been defined based on the tools that the country has acquired and international guidelines for pure entities: the LB, LDCGB, and the new entity of B lymphoma unclassifiable with features intermediate LDCGB and LB. The fluorescence in situ hybridization for the translocation (8;14) has been implemented in paraffin tissues for proper categorization. A total of 21 cases have been studied: the characteristics of patients, morphology, immunohistochemistry and the presence or absence of the translocation (8;14). Twelve of the cases have been classified as B-cell lymphoma unclassifiable with features intermediate between LDCGB and LB. Furthermore, nine of the cases were classified in LB. Fluorescence in situ hybridization (FISH) has been negative in 5 of the 21 cases. The diagnosis of lymphoma with features bordering between the LB and the LDCGB has been imperative for the survival of the patient and the corresponding treatment [es

  15. Studies of the Ecophysiology of Single Cells in Microbial Communities by (Quantitative) Microautoradiography and Fluorescence In Situ Hybridization (MAR-FISH)

    DEFF Research Database (Denmark)

    Nierychlo, Marta; Nielsen, Jeppe Lund; Nielsen, Per Halkjær

    2015-01-01

    image analysis (MARQuant). Quantification of MAR signals can answer more specific questions regarding metabolic activity and function of the microbes. Here, we give an overview of how to use MAR-FISH in various ecosystems and provide a detailed protocol for MAR-FISH, including sampling, incubation...

  16. KARAKTERISASI PROTEIN MIOFIBRIL DARI IKAN KUNIRAN (Upeneus moluccensis DAN IKAN MATA BESAR (Selar crumenophthalmus [Characterization of Myofibrillar Protein from Goldband Goat Fish (Upeneus moluccensis and Bigeye Scad Fish (Selar crumenophthalmus

    Directory of Open Access Journals (Sweden)

    Achmad Subagio

    2004-04-01

    Full Text Available Characteristics of myofibrillar protein from goldband goat fish (U. moluccensis and bigeye scad fish (S. crumenophthalmus were studied for their development as food ingredient. Color analysis using chromameter showed that myfibrillar protein from goldband goat fish was light colored, while that of bigeye scad was slightly drak colored. Proximate analysis showed that their contents were similar by crude protein 7-10%, crude fat 0.2-0.5%, and ash 0.4-0.7%. Amino acid compositions of both myofibrillar proteins were very close, dominated by glutamic acid (20%, aspartic acid (10% and lysine (9%. However, comparing with bigeye scad, myofibrillar proteins from goldband goatfish were easily aggregated, had higher gelation capacity and higher emulsion activity, but lower solubility. Based on these result, myofibrillar protein from goldband goatfish has good charachteristics as food ingredient especially for restructured products comparing with bigeye scad

  17. Petroleum hydrocarbons, fluorescent aromatic compounds in fish bile and organochlorine pesticides from areas surrounding the spill of the Kab121 well, in the Southern Gulf of Mexico: a case study.

    Science.gov (United States)

    Gold-Bouchot, G; Ceja-Moreno, V; Chan-Cocom, E; Zapata-Perez, O

    2014-01-01

    In October 2007, a light crude oil spill took place in the off shore Kab121 oil well, 32 km north of the mouth of the Grijalva River, Tabasco, Mexico. In order to estimate the possible effects of oil spill on the biota in the area surrounding the spilled well, the level of different fractions of petroleum hydrocarbons were measured in fish, as well as the concentration of some chlorinated hydrocarbons and PCBs. The organisms examined were cat fish (Ariopsis felis), in addition fluorescent aromatic compounds in bile, the contaminants above mentioned and their relationship with cyotochrome P-450 and Ethoxyresorufin-O-deethylase, Glutathion-S-Transferase and catalase activities in liver were determined. The concentration of most pollutants were low, except PAHs. Spatial distribution of these compounds, as well as most biomarkers, reflected the highest exposure of fish to pollutants in the area adjacent to well, as well as in the proximity of rivers. The profile of exposure to this environment was chronic in nature and not temporary.

  18. Identification and determination of the viability of Giardia lamblia cysts and Cryptosporidium parvum and Cryptosporidium hominis oocysts in human fecal and water supply samples by fluorescent in situ hybridization (FISH) and monoclonal antibodies.

    Science.gov (United States)

    Lemos, Vanessa; Graczyk, Thaddeus K; Alves, Margarida; Lobo, Maria Luísa; Sousa, Maria C; Antunes, Francisco; Matos, Olga

    2005-12-01

    In the present study, fluorescent in situ hybridization (FISH) and monoclonal antibodies (MAbs) were evaluated for species-specific detection and viability determination of Giardia lamblia, Cryptosporidium parvum, and Cryptosporidium hominis in human fecal and water supply samples. A total of 50 fecal human samples positive for G. lamblia cysts, 38 positive for C. parvum, and 23 positive for C. hominis were studied. Also, 18 water supply samples positive for Giardia spp. and Cryptosporidium spp. by the United States Environmental Protection Agency (USEPA) Method 1623 were studied by FISH and fluorescein isothiocyanate (FITC)-conjugated MAbs. Eighteen percent of the fecal samples parasitologically positive for G. lamblia presented viable and nonviable cysts, and 5% of those positive for Cryptosporidium spp. presented viable and nonviable oocysts. Of the 18 water supply samples analyzed, 6 (33%) presented Giardia spp. viable and nonviable cysts and 2 (11%) presented viable and nonviable Cryptosporidium spp. oocysts. G. lamblia identification was confirmed by polymerase chain reaction (PCR) and sequencing of the beta-giardin gene in the fecal and water samples found positive by FISH and FITC-conjugated MAbs. C. parvum and Cryptosporidium muris were identified, by PCR and sequencing of the small subunit of ribosomal RNA gene, in seven and one water samples, respectively. Our results confirm that this technique enables simultaneous visualization, species-specific identification, and viability determination of the organisms present in human fecal and water supply samples.

  19. Fish Allergy

    Science.gov (United States)

    ... Cause Blog Vision Awards Common Allergens Fish Allergy Fish Allergy Learn about fish allergy, how to read ... that you must avoid both. Allergic Reactions to Fish Finned fish can cause severe and potentially life- ...

  20. Mercury speciation in thawed out and refrozen fish samples by gas chromatography coupled to inductively coupled plasma mass spectrometry and atomic fluorescence spectroscopy

    NARCIS (Netherlands)

    Krystek, Petra; Ritsema, Rob

    Different sub-sampling procedures were applied for the determination of mercury species (as total mercury Hg, methylmercury MeHg+ and inorganic mercury Hg2+) in frozen fish meat. Analyses were carried out by two different techniques. After the sample material was pre-treated by microwave digestion,

  1. Fish Proteins as Targets of Ferrous-Catalyzed Oxidation: Identification of Protein Carbonyls by Fluorescent Labeling on Two-Dimensional Gels and MALDI-TOF/TOF Mass Spectrometry

    DEFF Research Database (Denmark)

    Pazos, Manuel; da Rocha, Angela Pereira; Roepstorff, Peter

    2011-01-01

    Protein oxidation in fish meat is considered to affect negatively the muscle texture. An important source of free radicals taking part in this process is Fenton's reaction dependent on ferrous ions present in the tissue. The aim of this study was to investigate the susceptibility of cod muscle pr...

  2. Role of immunohistochemistry and fluorescence in-situ hybridization (FISH in the diagnosis of spindle and round cell tumors of the kidney

    Directory of Open Access Journals (Sweden)

    M. Abbas

    2015-09-01

    Conclusion: In summary we advise an immunohistochemical panel for round/spindle cell tumors of the kidney and for unclear cases we advise to add (FISH to get the correct diagnosis, as they are completely different regarding surgical approach and post-operative adjuvant therapy.

  3. ILLEGAL FISHING SEBAGAI KEJAHATAN KORPORASI SUATU TEROBOSAN HUKUM PIDANA MENGADILI KEJAHATAN ILLEGAL FISHING

    Directory of Open Access Journals (Sweden)

    Moch. Iqbal

    2012-11-01

    Full Text Available Cakupan, pengertian dan pemahaman illegal fishing yang dipahami dan dianut dalam hukum positif Indonesia adalah pencurian ikan yang berdampak dan berpengaruh besar terhadap perekonomian negara. Oleh karenanya esensi regulasi dan penindakan terhadap illegal fishing di Indonesia seharusnya dipahami pada penindakan pencurian ikan yang berskala besar, yang pada umumnya dilakukan oleh armada-armada asing, kapal-kapal asing yang illegal, yang telah pula berakibat merugikan perekonomian negara (Indonesia triliunan rupiah tiap tahunnya. Dengan model dan jenis pencurian yang berskala besar dan membahayakan perekonomian nasional tersebut, penanganan regulasinya patut diarahkan pada atau untuk mengatasi kejahatan-kejahatan berskala besar dan rumit (sophisticated, yang tergolong dalam jenis kejahatan white collar crime atau kejahatan korporasi. Mengenal dan memahami seluk beluk serta keberadaan kejahatan korporasi, bagi para penegak hukum adalah sebuah keharusan di era modern ini, oleh karenanya tulisan ini memfokuskan pada dua kata kunci (key word illegal fishing dan corporate crime. Mengetahui dan memahami konsep dan regulasi seputar illegal fishing serta kejahatan korporasi menjadi kontribusi penting dalam proses penegakan hukum. Dengan pemahaman atas illegal fishing dan kejahatan korporasi akan menjadi landasan yang kuat bagi setiap praktisi hukum, khususnya penegak hukum dalam berproses acara maupun menentukan jenis dan klasifikasi pertanggungjawaban pidana yang tepat dan benar bagi penegak hukum khususnya para hakim agar berani dan tidak ragu dalam menjatuhkan sanksi pada setiap pelaku illegal fishing atau pelaku kejahatan korporasi. Dengan hukuman yang tegas dan jelas serta pasti, para penegak hukum tidak saja menegakkan hukum dan keadilan melainkan juga telah menyelamatkan perekonomian negara, dengan mencegah potensi kehilangan kekayaan nasional, sebagai pelaksanaan dari fungsi hukum sebagai alat rekayasa sosial.   Coverage, insight, and

  4. Detection of MDM2/CDK4 amplification in lipomatous soft tissue tumors from formalin-fixed, paraffin-embedded tissue: comparison of multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH).

    Science.gov (United States)

    Creytens, David; van Gorp, Joost; Ferdinande, Liesbeth; Speel, Ernst-Jan; Libbrecht, Louis

    2015-02-01

    In this study, the detection of MDM2 and CDK4 amplification was evaluated in lipomatous soft tissue tumors using multiplex ligation-dependent probe amplification (MLPA), a PCR-based technique, in comparison with fluorescence in situ hybridization (FISH). These 2 techniques were evaluated in a series of 77 formalin-fixed, paraffin-embedded lipomatous tumors (27 benign adipose tumors, 28 atypical lipomatous tumors/well-differentiated liposarcomas, 18 dedifferentiated liposarcomas, and 4 pleomorphic liposarcomas). Using MLPA, with a cut-off ratio of >2, 36/71 samples (22 atypical lipomatous tumors/well-differentiated liposarcomas, and 14 dedifferentiated liposarcomas) showed MDM2 and CDK4 amplification. Using FISH as gold standard, MLPA showed a sensitivity of 90% (36/40) and a specificity of 100% (31/31) in detecting amplification of MDM2 and CDK4 in lipomatous soft tissue tumors. In case of high-level amplification (MDM2-CDK4/CEP12 ratio >5), concordance was 100%. Four cases of atypical lipomatous tumor/well-differentiated liposarcoma (4/26, 15%) with a low MDM2 and CDK4 amplification level (MDM2-CDK4/CEP12 ratio ranging between 2 and 2.5) detected by FISH showed no amplification by MLPA, although gain of MDM2 and CDK4 (ratios ranging between 1.6 and 1.9) was seen with MLPA. No amplification was detected in benign lipomatous tumors and pleomorphic liposarcomas. Furthermore, there was a very high concordance between the ratios obtained by FISH and MLPA. In conclusion, MLPA proves to be an appropriate and straightforward technique for screening MDM2/CDK4 amplification in lipomatous tumors, especially when a correct cut-off value and reference samples are chosen, and could be considered a good alternative to FISH to determine MDM2 and CDK4 amplification in liposarcomas. Moreover, because MLPA, as a multiplex technique, allows simultaneous detection of multiple chromosomal changes of interest, it could be in the future a very reliable and fast molecular analysis on

  5. Assessment of DNA damage and Chromosome aberration in human lymphocyte exposed to low dose radiation detected by FISH(Fluorescence In Situ Hybridization) and SCGE(Single Cell Gel Electrophoresis)

    International Nuclear Information System (INIS)

    Chung, Hai Won; Kim, Su Young; Kim, Byung Mo; Kim, Sun Jin; Ha, Sung Whan; Kim, Tae Hwan; Cho, Chul Koo

    2000-01-01

    Comparative study was performed for the assessment of DNA damage and Chromosomal aberration in human lymphocyte exposed to low dose radiation using Fluorescence In Situ Hybridization(FISH) and Single Cell Gel Electrophoresis(SCGE). Chromosomal aberrations in human lymphocyte exposed to radiation at doses of 5, 10, 30 and 50cGy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. FISH with chromosome-specific probe has been used to be a valid and rapid method for detection of chromosome rearrangements induced by low dose radiation. The frequencies of stable translocation per cell equivalents were 0.0116, 0.0375, 0.0407, 0.0727 and 0.0814 for 0, 5, 10, 30 and 50cGy, respectively, and those of dicentric were 0.00, 0.0125, 0.174, 0.0291 and 0.0407 respectively. Radiation induced DNA damage in human lymphocyte in a dose-dependent manner at low doses from 5cGy to 50cGy, which were analysed by single Cell Gel Electrophoresis(SCGE). From above results, FISH seemed to be useful for radiation biodosimetry by which the frequencies of stable aberrations in human lymphocyte can be observed more easily than by conventional method and SCGE also seemed to be sensitive method for detecting DNA damage by low dose radiation exposure, so that those methods will improve our technique to perform meaningful biodosimetry for radiation at low doses

  6. Fish proteins as targets of ferrous-catalyzed oxidation: identification of protein carbonyls by fluorescent labeling on two-dimensional gels and MALDI-TOF/TOF mass spectrometry.

    Science.gov (United States)

    Pazos, Manuel; da Rocha, Angela Pereira; Roepstorff, Peter; Rogowska-Wrzesinska, Adelina

    2011-07-27

    Protein oxidation in fish meat is considered to affect negatively the muscle texture. An important source of free radicals taking part in this process is Fenton's reaction dependent on ferrous ions present in the tissue. The aim of this study was to investigate the susceptibility of cod muscle proteins in sarcoplasmic and myofibril fractions to in vitro metal-catalyzed oxidation and to point out protein candidates that might play a major role in the deterioration of fish quality. Extracted control proteins and proteins subjected to free radicals generated by Fe(II)/ascorbate mixture were labeled with fluorescein-5-thiosemicarbazide (FTSC) to tag carbonyl groups and separated by two-dimensional gel electrophoresis. Consecutive visualization of protein carbonyl levels by capturing the FTSC signal and total protein levels by capturing the SyproRuby staining signal allowed us to quantify the relative change in protein carbonyl levels corrected for changes in protein content. Proteins were identified using MALDI-TOF/TOF mass spectrometry and homology-based searches. The results show that freshly extracted cod muscle proteins exhibit a detectable carbonylation background and that the incubation with Fe(II)/ascorbate triggers a further oxidation of both sarcoplasmic and myofibril proteins. Different proteins exhibited various degrees of sensitivity to oxidation processes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), nucleoside diphosphate kinase B (NDK), triosephosphate isomerase, phosphoglycerate mutase, lactate dehydrogenase, creatine kinase, and enolase were the sarcoplasmic proteins most vulnerable to ferrous-catalyzed oxidation. Moreover, NDK, phosphoglycerate mutase, and GAPDH were identified in several spots differing by their pI, and those forms showed different susceptibilities to metal-catalyzed oxidation, indicating that post-translational modifications may change the resistance of proteins to oxidative damage. The Fe(II)/ascorbate treatment significantly

  7. The application of fluorescence in situ hybridization (FISH technique for studying the microbial communities in intestinal tissues of white shrimp (Penaeus vannamei

    Directory of Open Access Journals (Sweden)

    Supamattaya, K.

    2005-02-01

    Full Text Available Fluorescence in situ hybridization technique is very useful for the evaluation of microbial communities in various environments. It is possible to apply this technique to study the intestinal microflora in white shrimp (Penaeus vannamei. Different fixatives and storage temperature were tested in this technique. It was found that fixation with 10% buffered formalin for 12 hours and changed to 70% ethanol shown positive results when compared to the fixation with Davidson's fixative or RF fixative. The best signaling was obtainedfrom the samples which were stored in -20ºC. By using the DNA probe targeted to the Eubacteria domain (EUB338 probe, 5′-GCT GCC TCC CGT AGG AGT-3′ labeled with fluorescein as a hybridizing probe, it was found that most intestinal microflora were aggregated with the intestinal contents, or dispersed in the lumen. There was not evidence of the attachment of the microflora with the intestinal epithelium in this study.

  8. Fluorescence spectroscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2016-01-01

    Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses the foundati......Fluorescence spectroscopy is a powerful experimental tool used by scientists from many disciplines. During the last decades there have been important developments on distinct fluorescence methods, particularly those related to the study of biological phenomena. This chapter discusses...

  9. Detection of {open_quotes}cryptic{close_quotes}karyotypic rearrangements in closely related primate species by fluorescence in situ hybridization (FISH) using human subtelomeric DNA probes

    Energy Technology Data Exchange (ETDEWEB)

    Youngblom, J.J. [California State University-Stanislaus, Turlock, CA (United States); Trask, B.J.; Friedman, C. [Univ. of Washington, Seattle, WA (United States)] [and others

    1994-09-01

    Specific human subtelomeric DNA probes were used to reveal cryptic chromosomal rearrangements that cannot be detected by conventional high resolution cytogenetic techniques, or by chromosomal in situ suppression hybridization using whole chromosome paint analysis. Two cosmids containing different subtelomeric DNA sequences were derived from human chromosome 19 and designated as 7501 and 16432. Cosmid 7501 was hybridized to chromosomes from humans, chimpanzee, gorilla and orangutan. In humans, 7501 consistently labeled chromosomes 3q, 15q, and 19p. Additional chromosomes were labeled in different individuals, indicating a polymorphic distribution of this sequence in the human genome. In contrast, 7501 consistently and strongly labeled only the q arm terminus of chromosome 3 in both chimp and gorilla. The identification of the chromosome was made by two-color FISH analysis using human chromosome 4-specific paint and homologous to human chromosome 4. None of the human subjects showed labeling of chromosome 4 with 7501. This finding suggests that in the course of human evolution, subsequent to the divergence of humans and African apes, a cryptic translocation occurred between the ancestral human chromosome 4 and one or more of the other human chromosomes that now contain this DNA segment. In orangutan, 7501 labeled a single acrocentric chromosome pair, a distinctly different chromosome than that labeled in chimp and gorilla. Comparison of chromosome sites labeled with cosmid 16432 showed the distribution of signals on chromosome 1q arm is the same for humans and chimp, but different in the gorilla. Humans and chimps show distinct labeling on sites 1q terminus and 1q41-42. In gorilla, there is instead a large cluster of intense signal near the terminus of 1q that clearly does not extend all the way to the terminus. A paracentric inversion or an unequal cross-over event may account for the observed difference between these species.

  10. KASUS DISTRIBUSI DAN PENGGUNAAN FORMALIN DALAM PENGAWETAN KOMODITI IKAN LAUT SEGAR (STUDI KASUS DI KOTA BANDAR LAMPUNG [Formaldehyde Distribution and Using for Preserving Fresh Fish (A Case Study in Bandar Lampung City

    Directory of Open Access Journals (Sweden)

    Dias Yusdianson Girsang

    2014-12-01

    Full Text Available The purpose of this study was to identify the presence of formaldehyde in some fresh fish commodities and to trace formaldehyde distribution in Bandar Lampung City. The  formadelhyde tests were done  on the storage water of fresh fish samples. The study conducted in July to October 2013 was devided in two stages: a survey to fill out a questionnaire to a number of respondents, followed by sampling some storage water of fresh fish to be tested in the laboratory.  The tests were  carried out on 52 sampling points taken from the fishing boats in the fishing landing port (17 boats, fish supplier car from outside Bandar Lampung (6 cars as well as some

  11. KASUS DISTRIBUSI DAN PENGGUNAAN FORMALIN DALAM PENGAWETAN KOMODITI IKAN LAUT SEGAR (STUDI KASUS DI KOTA BANDAR LAMPUNG [Formaldehyde Distribution and Using for Preserving Fresh Fish (A Case Study in Bandar Lampung City

    Directory of Open Access Journals (Sweden)

    Dias Yusdianson Girsang

    2014-12-01

    Full Text Available The purpose of this study was to identify the presence of formaldehyde in some fresh fish commodities and to trace formaldehyde distribution in Bandar Lampung City. The  formadelhyde tests were done  on the storage water of fresh fish samples. The study conducted in July to October 2013 was devided in two stages: a survey to fill out a questionnaire to a number of respondents, followed by sampling some storage water of fresh fish to be tested in the laboratory.  The tests were  carried out on 52 sampling points taken from the fishing boats in the fishing landing port (17 boats, fish supplier car from outside Bandar Lampung (6 cars as well as some fish sellers in 5 traditional market in Bandar Lampung (29 sellers. By conducting a laboratory test using Formaldehyde Test Kit, there were 2 samples that were purple (positively contain formaldehyde, which were taken from 2 boats in fish landing port of Lempasing. Then the assertion test was carried out to the positive samples using chromotropic acid (SNI 01 – 2894 – 1992. The results showed that the samples positively contained formaldehyde indeed, which was characterized by a bluish purple color. Based on the survey results and tracking, there was a distortion of formaldehyde distribution in Bandar Lampung, where domestic industries (including fisherman obtained formaldehyde illegally, either from End Users, a local manufacturer or drugstore/hospital/other health care facilities. Keywords: formaldehyde, fresh fish storage water, distribution distortion.

  12. KASUS DISTRIBUSI DAN PENGGUNAAN FORMALIN DALAM PENGAWETAN KOMODITI IKAN LAUT SEGAR (STUDI KASUS DI KOTA BANDAR LAMPUNG Formaldehyde Distribution and Using for Preserving Fresh Fish (A Case Study in Bandar Lampung City

    Directory of Open Access Journals (Sweden)

    Dias Yusdianson Girsang

    2014-12-01

    Full Text Available The purpose of this study was to identify the presence of formaldehyde in some fresh fish commodities and to trace formaldehyde distribution in Bandar Lampung City. The  formadelhyde tests were done  on the storage water of fresh fish samples. The study conducted in July to October 2013 was devided in two stages: a survey to fill out a questionnaire to a number of respondents, followed by sampling some storage water of fresh fish to be tested in the laboratory.  The tests were  carried out on 52 sampling points taken from the fishing boats in the fishing landing port (17 boats, fish supplier car from outside Bandar Lampung (6 cars as well as some fish sellers in 5 traditional market in Bandar Lampung (29 sellers. By conducting a laboratory test using Formaldehyde Test Kit, there were 2 samples that were purple (positively contain formaldehyde, which were taken from 2 boats in fish landing port of Lempasing. Then the assertion test was carried out to the positive samples using chromotropic acid (SNI 01 – 2894 – 1992. The results showed that the samples positively contained formaldehyde indeed, which was characterized by a bluish purple color. Based on the survey results and tracking, there was a distortion of formaldehyde distribution in Bandar Lampung, where domestic industries (including fisherman obtained formaldehyde illegally, either from End Users, a local manufacturer or drugstore/hospital/other health care facilities. Key word: formaldehyde, fresh fish storage water, distribution distortion.

  13. Fish Allergy

    Science.gov (United States)

    ... Safe Videos for Educators Search English Español Fish Allergy KidsHealth / For Parents / Fish Allergy What's in this ... Print en español Alergia al pescado About Fish Allergy A fish allergy is not exactly the same ...

  14. Fish allergy and fish allergens

    DEFF Research Database (Denmark)

    Kuehn, A; Hilger, Christiane; Ollert, Markus

    2016-01-01

    Fish is one of the main elicitors for food allergies. For a long time, the clinical picture of fish allergy was reduced to the following features. First, fish-allergic patients suffer from a high IgE cross-reactivity among fishes so that they have to avoid all species. Second, clinically relevant...... symptoms are linked to the presence of IgE-antibodies recognizing parvalbumin, the fish panallergen. This view was challenged by results from recent studies as follows. 1. Allergic reactions which are limited to single or several fish species (mono-or oligosensitisations) apply not only to single cases...... but patients with this phenotype constitute an important sub-group among fish-allergic individuals. 2. Newly identified fish allergens, enolases, aldolases, and fish gelatin, are of high relevance as the majority of the fish-allergic individuals seem to develop specific IgE against these proteins. The present...

  15. SEBARAN LARVA IKAN DAN KAITANNYA DENGAN KONDISI OSEANOGRAFI LAUT SULAWESI

    Directory of Open Access Journals (Sweden)

    Khairul Amri

    2015-06-01

    Full Text Available Laut Sulawesi diketahui sebagai daerah penangkapan ikan yang potensial sekaligus diduga sebagai lokasi pemijahan. Berbagai jenis larva ikan pelagis maupun demersal ditemukan di perairan ini. Kelimpahan dan sebaran larva ikan di suatu perairan sangat dipengaruhi oleh kondisi oseanografi seperti temperatur, salinitas dan sejumlah parameter lainnya termasuk ketersediaan pakan. Untuk mengetahui pengaruh parameter oseanografi terhadap kelimpahan dan sebaran spasial larva ikan di Laut Sulawesi, telah dilakukan penelitian menggunakan kapal riset KR Baruna Jaya VII pada Oktober 2012. Parameter oseanografi yaitu temperatur dan salinitas diukur menggunakan iCTD dan sampling larva menggunakan bonggo net pada 18 stasiun pengukuran. Analisa hubungan kondisi oseanografi dengan sebaran larva dilakukan secara deskriptif dan pemetaan sebarannya dilakukan secara spasial. Hasil menunjukan keterkaitan sejumlah parameter oseanografi dengan kelimpahan dan sebaran spasial larva ikan. Sebaran larva famili Scombroidae dominan berada pada perairan bersalinitas tinggi karena merupakan jenis ikan oseanik. Larva ikan demersal banyak ditemukan di perairan sekitar Kep.Sangihe Talaud. Kelimpahan larva tertinggi ditemukan di perairan bagian utara dan barat lokasi penelitian dimana kelimpahan plankton tinggi ditemukan.   Celebes Sea is known as a potential fishing and spawning grounds for several pelagic fish species. Abundance and distribution of fish larvae are allegedly linked to oceanographic conditions such as temperature, salinity and others oceanographic parameters including food availablity. To see the effect of oceanographic on the abundance and spatial distribution of fish larvae in the Celebes Sea, has conducted a research in October 2012using the research vessel KR Baruna Jaya VII. The measurement of oceanographic parameters including temperature and salinity and larval sampling were done respectively by using iCTD and Bonggo net at 18 measuring stations. The

  16. Novel dansyl-appended calix[4]arene frameworks: fluorescence properties and mercury sensing.

    Science.gov (United States)

    Pandey, Shubha; Azam, Amir; Pandey, Siddharth; Chawla, H M

    2009-01-21

    Covalently-attached fluorophores may impart enhanced chemosensing capabilities to calixarene frameworks. Synthesis and characterization of six novel dansyl-appended calix[4]arenes, namely, H/Dan4, NO2/Dan4, H/(OH)2Dan2, H/(Ester)2(Dan)2, t-Bu/(OH)2Dan2, and t-Bu/(Ester)2Dan2, containing two or four dansyl moieties are reported. Among these, fluorescence intensity of NO2/Dan4 is observed to decrease significantly in the presence Hg2+ in the solution. Based on the decrease in fluorescence, a limit of detection for Hg2+ of 20 ppb is obtained. NO2/Dan4 as a chemosensing agent for Hg2+ shows excellent selectivity and adequate reversibility. Complexation of NO2/Dan4 with Hg2+ is investigated using fluorescence spectroscopy and is observed to be 2:1. The formation constant of (NO2/Dan4)2Hg2+ is estimated to be 5.2(+/- 0.8) x 10(10) M(-2) at ambient conditions. These observations are traced to the fact that while all other dansyl-appended calix[4]arenes show cone conformation in the solution, NO2/Dan4 is in the 1,3-alternate conformation. Stokes shift versus solvent orientational polarizability for NO2/Dan4 also indicates the difference in the ground- to excited-state dipole moment of this compound to be the maximum among all six, rendering it most sensitive to its environment. Fluorescence emission of NO2/Dan4 in nonpolar chloroform, polar-aprotic acetonitrile, and polar-protic ethanol is observed to be different than that of the rest of the dansyl-appended compounds as well.

  17. Environmental monitoring of the La Grande complex (2003-2004) : evolution of mercury levels in the flesh of fish; Reseau de suivi environnemental du complexe La Grande (2003-2004) : evolution du mercure dans la chair des poissons

    Energy Technology Data Exchange (ETDEWEB)

    Therrien, J. [Genivar SEC, Quebec, PQ (Canada); Schetagne, R. [Hydro-Quebec Production, Baie-Comeau, PQ (Canada)

    2005-11-15

    The results of surveys conducted to assess the duration of temporary mercury levels in piscivorous species in the La Grande Complex were presented. A 2003 survey conducted in the easter sector and a 2004 survey conducted in the western sector of the complex showed that for non-piscivorous fishes of standardized length, a return to mean natural mercury levels will be achieved between 10 and 20 years after impounding. For piscivorous fishes, the evolution pattern of the mean mercury levels suggested that a return to background levels will occur after 20 to 30 years. Mercury levels for northern pike in the Robert-Bourassa Reservoir are expected to return to normal levels after 30 to 35 years. The surveys indicated that mean mercury levels in non-piscivorous fishes were often higher immediately below the La Grande generating stations. Similar observations were made for northern pike and lake trout downstream of the generating stations in the eastern sector of the complex. Mean mercury levels were significantly higher for fishes in the complex than fishes in the natural lakes of the region. Results of the surveys suggested that additional consumption restrictions for piscivorous fishes in the reservoirs are needed. Consumption guidelines for varieties of non-piscivorous and piscivorous fishes from the complex were included.

  18. Meet EPA's Dan Nelson

    Science.gov (United States)

    EPA’s Dan Nelson is the Director of the Human Research Protocol Office at the National Health and Environmental Effect Research Laboratory, Dan works to protect the rights and welfare of EPA’s research participants.

  19. Bahasa, Simbol, dan Religi

    OpenAIRE

    Supriyadi, Supriyadi

    1999-01-01

    Manusia sering disebut sebagai makhluk sosial, makhluk berakal, makhluk berseni, dan sebagainya. Tulisan ini secara tidak langsung membicarakan manusia sebagai makhluk berbahasa, bersimbol, dan bereligi . Meskipun demikian, titik tolaknya bukan pada manusia itu sendiri, tetapi pada bahasa, simbol, dan religinya, terutama pada hubungan di antara ketiganya . Hubungan antara bahasa dan simbol tidak diragukan lagi karena bahasa merupakan jenis simbol . Demikian juga hubungan antara simbol dengan ...

  20. Fish Rhabdoviruses

    Science.gov (United States)

    Kurath, G.; Winton, J.

    2008-01-01

    Many important viral pathogens of fish are members of the family Rhabdoviridae. The viruses in this large group cause significant losses in populations of wild fish as well as among fish reared in aquaculture. Fish rhabdoviruses often have a wide host and geographic range, and infect aquatic animals in both freshwater and seawater. The fish rhabdoviruses comprise a diverse collection of isolates that can be placed in one of two quite different groups: isolates that are members of the established genusNovirhabdovirus, and those that are most similar to members of the genus Vesiculovirus. Because the diseases caused by fish rhabdoviruses are important to aquaculture, diagnostic methods for their detection and identification are well established. In addition to regulations designed to reduce the spread of fish viruses, a significant body of research has addressed methods for the control or prevention of diseases caused by fish rhabdoviruses, including vaccination. The number of reported fish rhabdoviruses continues to grow as a result of the expansion of aquaculture, the increase in global trade, the development of improved diagnostic methods, and heightened surveillance activities. Fish rhabdoviruses serve as useful components of model systems to study vertebrate virus disease, epidemiology, and immunology.

  1. DISTRIBUSI NITROGEN DAN FOSFOR PADA BUDIDAYA IKAN GABUS (Channa striata DENGAN APLIKASI ECENG GONDOK (Eichhornia crassipes DAN PROBIOTIK

    Directory of Open Access Journals (Sweden)

    Adang Saputra

    2018-01-01

    Full Text Available Permasalahan yang dihadapi pembudidaya ikan dengan sistem intensif adalah meningkatnya limbah yang terakumulasi pada air dan sedimen. Limbah budidaya ikan pada umumnya berupa padatan dan nutrien terlarut pada air terutama nitrogen dan fosfor. Tujuan penelitian ini adalah mengkaji distribusi nitrogen total dan fosfor total pada budidaya ikan gabus secara intensif yang diberi eceng gondok Eichhornia crassipes dan probiotik (Pseudomonas aeruginosa dan Achromobacter insuavis. Penelitian dirancang menggunakan rancangan acak lengkap dengan perlakuan pemberian kombinasi eceng gondok dan probiotik (A, pemberian eceng gondok (B, dan pemberian probiotik (C, masing-masing perlakuan diulang tiga kali. Benih ikan gabus yang digunakan berukuran panjang 14,74 ± 0,01 cm dan bobot 25,53 ± 0,09 g dengan padat tebar 175 ekor/kolam (50 ekor/m3. Selama 90 hari masa pemeliharaan, ikan gabus diberi pakan berupa pelet dengan kandungan protein sekitar 30%. Jumlah pemberian pakan 5% dari biomassa dengan frekuensi pemberian empat kali dalam sehari (pagi, siang, sore, dan malam. Hasil penelitian menunjukkan nitrogen dan fosfor pada budidaya ikan gabus terdistribusi pada eceng gondok, sedimen, air, dan ikan. Eceng gondok menyerap nitrogen dan fosfor paling tinggi (P<0,05 dibandingkan air, ikan, dan sedimen. Laju pertumbuhan spesifik bobot (4,37 ± 0,01%/hari dan biomassa (1,88 ± 0,01 g ikan gabus tertinggi dicapai pada pemberian kombinasi eceng gondok dan probiotik. Hasil ini dapat dijadikan landasan untuk pengelolaan limbah nitrogen dan fosfor pada budidaya ikan gabus secara intensif. One of the problems in intensive aquaculture system is the the accumulation of waste in the water and sediment. Aquaculture wastes are discharged into the water in form of solids and dissolved nutrients which mostly consisted of nitrogen and phosphorus. The purpose of this study was to study the dynamics of total nitrogen and phosphorus in an intensive aquaculture media supplied with water

  2. Fluorescence microscopy.

    Science.gov (United States)

    Sanderson, Michael J; Smith, Ian; Parker, Ian; Bootman, Martin D

    2014-10-01

    Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution. The basics of wide-field microscopy are outlined to emphasize the selection, advantages, and correct use of laser scanning confocal microscopy, two-photon microscopy, scanning disk confocal microscopy, total internal reflection, and super-resolution microscopy. In addition, the principles of how these microscopes form images are reviewed to appreciate their capabilities, limitations, and constraints for operation. © 2014 Cold Spring Harbor Laboratory Press.

  3. Microbial abundance and diversity in water, and immune parameters of red tilapia reared in bioflocs system with different fish density (25 fish/m3, 50 fish/m3, and 100 fish/m3

    Directory of Open Access Journals (Sweden)

    Frid Agustinus

    2010-07-01

    Full Text Available ABSTRACTThe objective of this experiment was to study microbial abundance and diversity in the water, and immune parameters of red tilapia Oreochromis sp. cultured in bioflok system with different fish stocking densities. The experiment comprised of two different factors, carbon source addition (bioflocs and control, and fish stocking density (25 fish/m3, 50 fish/m3, dan 100 fish/m3, with an experimental period of 99 days. Microbial load in water was determined biweekly, whereas immune parameters represented by fish blood profile were measured on day 0, 50, and 90. There was no significant difference in total bacteria count in the water of all treatments; there was however a tendency shown by all treatments that the microbial load in water increased along with the culture period. There were 4 genera of bacteria which particularly found in bioflok system, which are Acinetobacter sp., Corynobacterium sp., Listeria sp., dan Pseudomonas sp, and are suggested to play a role in bioflok formation. The percentage of phagocytic index of fish in bioflok system was higher than that in control, and may indicate that bioflok may stimulate the fish immune system.Keywords: bioflocs, red tilapia, bacteria, blood profile. ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi kelimpahan dan keragaman jenis bakteri dalam air dan parameter imunitas ikan nila Oreochromis sp. yang dipelihara dalam sistem bioflok dengan kepadatan ikan yang berbeda. Penelitian terdiri atas dua faktor perlakuan yaitu penambahan sumber carbon (bioflok dan kontrol, dan padat penebaran ikan (25 ekor/m3, 50 ekor/m3, dan 100 ekor/m3 dengan lama waktu pemeliharaan ikan selama 99 hari. Kelimpahan bakteri diukur setiap 2 minggu sekali selama masa pemeliharaan. Parameter imunitas meliputi gambaran darah diukur dengan pengambilan contoh darah yang dilakukan pada tiga ekor ikan pada hari ke 0, 50, dan 99. Kelimpahan bakteri pada semua perlakuan pada setiap titik pengamatan tidak menunjukkan

  4. Analisis Kebijakan dan Efektivitas Organisasi

    Directory of Open Access Journals (Sweden)

    Amir Syarifudin Kiwang

    2015-05-01

    Full Text Available Penerapan Peraturan Gubernur Nusa Tenggara Timur Nomor 8 Tahun 2011 tentang Organisasi dan Tata Kerja Unit Pelaksana Teknis Pendidikan dan Pelatihan Koperasi dan Usaha Mikro Kecil dan Menengah pada Dinas Koperasi dan Usaha Mikro Kecil dan Menengah Provinsi Nusa Tenggara Timur adalah dalam rangka untuk meningkatkan efektivitas kerja organisasi pada UPT Diklat Koperasi dan UMKM. Penelitian ini berusaha untuk melihat dampak kebijakan organisasi terhadap efektivitas organisasi dengan menggunakan enam elemen variabel dari kebijakan dan praktik manajemen. Keenam elemen tersebut adalah penetapan tujuan strategis, pencarian dan pemanfaatan sumber daya, lingkungan prestasi, proses komunikasi, kepemimpinan dan pengambilan keputusan, serta adaptasi dan inovasi organisasi. Penelitian kualitatif ini menggunakan pendekatan studi kasus. Teknik analisis data yang digunakan adalah analisis data model Miles dan Huberman, yang terdiri atas reduksi data, display/penyajian data, dan kesimpulan/verifikasi. Hasil penelitian menunjukkan bahwa efektivitas organisasi pada UPT Diklat koperasi dan UMKM belum berjalan secara baik, hal ini dapat dilihat dari beberapa hal antara lain, belum adanya tenaga spesialisasi pengelola diklat dan tenaga khusus (sarjana di bidang perkoperasian dan kondisi lingkungan kerja (lingkungan prestasi pada UPT Diklat koperasi dan UMKM yang belum efektif. Penempatan pegawai juga belum tepat, mutasi ke UPT Diklat Koperasi dan UMKM tidak memperhatikan latar belakang pendidikan dan spesialisasi/keahlian pegawai sehingga membutuhkan waktu dalam proses penyesuaian serta menurunkan jumlah pelaksanaan diklat dikarenakan keterbatasan alokasi dana.

  5. APLIKASI TEKNIK AAN DI REAKTOR RSG-GAS PADA PENENTUAN UNSUR ESENSIAL DAN TOKSIK DI DALAM IKAN DAN PAKAN IKAN

    Directory of Open Access Journals (Sweden)

    Saeful Yusuf

    2015-03-01

    Full Text Available Pada makalah ini diuraikan tentang aplikasi teknik AAN (Analisis Aktivasi Neutron dalam penentuan konsentrasi unsur-unsur esensial dan cemaran yang terkandung di dalam beberapa spesies ikan dan pakan ikan. Unsur-unsur esensial yang terkandung dalam pakan ikan buatan juga dianalisis untuk mengetahui pengaruhnya terhadap ikan. Penentuan unsur menggunakan teknik AAN dengan metode perbandingan dan metode k0-AAN. Sampel diiradiasi di reaktor RSG-GAS yang memiliki fluks neutron thermal 5 x 1013 n.cm-2.s-1 pada daya 15 MW. Hasil penelitian menunjukkan bahwa 12 unsur di dalam 11 spesies ikan air laut dan air tawar telah ditentukan yaitu As, Br, Cr, Co, Cs, Fe, Hg, K, Na, Rb, Se and Zn. Konsentrasi cemaran As didalam ikan laut sudah melampaui batas maksimum 1 mg/kg, sedangkan konsentrasi cemaran Hg masih dibawah batas maksimum 0,5 mg/kg, baik untuk ikan laut maupun ikan air tawar. Unsur K dan Na merupakan unsur makroesensial sedangkan unsur Cr, Co, Fe, Se and Zn adalah termasuk unsur mikroesensial. Secara umum ditunjukkan bahwa kandungan mineral didalam ikan laut lebih tinggi konsentrasinya dibandingkan ikan air tawar. Br, Cs dan Rb merupakan unsur-unsur non esensial yang teridentifikasi dalam semua ikan yang dianalisis. Penelitian terhadap pakan ikan air tawar menunjukkan bahwa semua unsur yang teridentifikasi juga terdapat di dalam ikan laut dan ikan air tawar. Hal ini menunjukkan bahwa pakan ikan berkontribusi terhadap konsentrasi unsur di dalam ikan air tawar. Kata kunci : Analisis aktivasi neutron, unsur esensial, unsur cemaran, ikan, pakan ikan   This paper reported on the application of NAA (Neutron Activation Analysis Technique in the determination of the concentration of the essential and toxic elements in some species of fish and fish feed. Determination of elements using instrumental NAA technique with comparison and k0-INAA methods. Samples were irradiated in the RSG-GAS which has a thermal neutron flux  5.0E +13 ncm-2s-1. The results

  6. Fish health and fish quality

    DEFF Research Database (Denmark)

    Ingerslev, Hans-Christian

    Aquaculture is an expanding worldwide industry producing an increasing amount of fish every year. The quality of the fish meat is dependent upon many biological and non-biological factors. Infectious diseases are known to cause bleedings and damage of the muscle tissue that may lead to scarring...... are poorly described in fish. The present work in this thesis focused on: 1) examination of potential changes in the quality regarding texture of the muscle tissue in rainbow trout (Oncorhynchus mykiss) after previous infection with the bacterial pathogens Yersinia ruckeri and Vibrio anguillarum; 2...... of these studies showed that previous infections by Yersinia ruckeri and Vibrio anguillarum gave rise to subsequent changes regarding textural quality parameters in fresh fish meat, while no differences were seen for cold-smoked meat from the same fish. The texture in previous infected fish was less flaky and less...

  7. TANGKI SEPTIK DAN MASALAHNYA

    Directory of Open Access Journals (Sweden)

    Sri Soewasti Soesanto

    2012-10-01

    Full Text Available Manusia yang sehat tiap hari membuang tinja yang harus ditampung dan/atau diolah secara saniter. Meskipun Indonesia telah 55 tahun merdeka dan telah memiliki banyak ahli sanitasi/teknik penyehatan baik lulusan dalam maupun luar negeri, namun cara pengelolaan tinja yang ada kebanyakan masih belum memenuhi syarat kesehatan. Tangki septik dianggap sebagai cara penampungan tinja yang terbaik, padahal sebenarnya masih terjadi pencemaran tanah dan air melalui saluran perembesan.Tangki septik (septic tank merupakan salah satu macam sarana pengolahan tinja manusia yang pada garis besarnya terdiri dari sebuah tangki pembusukan lumpur (sludge digester dan saluran perembesan efluen. Tangki pembusukan harus memenuhi syarat mengenai perbandingan panjang dan lebar serta syarat kedalaman maksimum dan minimum, agar pembusukan lumpur dari tinja manusia dapat berjalan sempurna malahan tidak berbau busuk lagi.

  8. Fish pelleting

    African Journals Online (AJOL)

    PUBLICATIONS1

    fish meal pelletizing machine utilized 4kg of ingredients to produce 3.77kg pellets at an effi- ciency of .... Design and fabrication of fish meal pellet processing machine ... 53 ... horsepower for effective torque application on .... two edges were tacked with a spot weld to hold ... then welded on to the shaft making sure that the.

  9. Fish parasites

    DEFF Research Database (Denmark)

    This book contains 22 chapters on some of the most important parasitic diseases in wild and farmed fish. International experts give updated reviews and provide solutions to the problems......This book contains 22 chapters on some of the most important parasitic diseases in wild and farmed fish. International experts give updated reviews and provide solutions to the problems...

  10. Fish reproduction

    National Research Council Canada - National Science Library

    Rocha, Maria João; Arukwe, Augustine; Kapoor, B. G

    2008-01-01

    ... of reproductive systems is essential for such studies. Fishes comprise over 28,000 species, with a remarkable variability in morphology, physiology and environmental adaptation. Knowledge on fish reproduction is scattered across numerous sources that shows a dynamic research field. The Editors believe it to be an opportune moment for a...

  11. DEPRESI DAN GANGGUAN TIDUR

    Directory of Open Access Journals (Sweden)

    Wayan Eko Radityo

    2012-12-01

    Full Text Available Depresi merupakan gangguan mood berupa kesedihan yang intens, berlangsung dalamwaktu lama, dan mengganggu kehidupan normal yang insidennya semakin meningkatseiring dengan meningkatnya tekanan hidup. Tahun 2020, depresi diperkirakanmenempati urutan kedua penyakit di dunia. Gejala-gejala depresi terdiri dari gangguanemosi, gangguan kognitif, keluhan somatik, gangguan psikomotor, dan gangguanvegetatif. Salah satu gejala depresi yang muncul adalah gangguan tidur yang bisaberupa insomnia, bangun secara tiba-tiba, dan hipersomnia. Hal ini disebabkan olehgangguan neurotransmiter dan regulasi hormon. Selain sebagai gejala depresi, gangguantidur juga bisa merupakan penyebab depresi. Beberapa penelitian memberikanhubungan gangguan tidur dapat meningkatkan risiko depresi di kemudian hari.

  12. Epidermal Growth Factor Receptor (EGFR) gene copy number (GCN) correlates with clinical activity of irinotecan-cetuximab in K-RAS wild-type colorectal cancer: a fluorescence in situ (FISH) and chromogenic in situ hybridization (CISH) analysis

    International Nuclear Information System (INIS)

    Scartozzi, Mario; Galizia, Eva; Berardi, Rossana; Biscotti, Tommasina; Labianca, Roberto; Masi, Gianluca; Falcone, Alfredo; Cascinu, Stefano; Bearzi, Italo; Mandolesi, Alessandra; Pierantoni, Chiara; Loupakis, Fotios; Zaniboni, Alberto; Negri, Francesca; Quadri, Antonello; Zorzi, Fausto

    2009-01-01

    K-RAS wild type colorectal tumors show an improved response rate to anti-EGFR monoclonal antibodies. Nevertheless 70% to 40% of these patients still does not seem to benefit from this therapeutic approach. FISH EGFR GCN has been previously demonstrated to correlate with clinical outcome of colorectal cancer treated with anti-EGFR monoclonal antibodies. CISH also seemed able to provide accurate EGFR GCN information with the advantage of a simpler and reproducible technique involving immunohistochemistry and light microscopy. Based on these findings we investigated the correlation between both FISH and CISH EGFR GCN and clinical outcome in K-RAS wild-type colorectal cancer treated with irinotecan-cetuximab. Patients with advanced K-RAS wild-type, colorectal cancer receiving irinotecan-cetuximab after failure of irinotecan-based chemotherapy were eligible. A cut-off value for EGFR GCN of 2.6 and 2.12 for FISH and CISH respectively was derived from ROC curve analysis. Forty-four patients were available for analysis. We observed a partial remission in 9 (60%) and 2 (9%) cases with a FISH EGFR GCN ≥ 2.6 and < 2.6 respectively (p = 0.002) and in 10 (36%) and 1 (6%) cases with a CISH EGFR GCN ≥ 2.12 and < 2.12 respectively (p = 0.03). Median TTP was 7.7 and 6.4 months in patients showing increased FISH and CISH EGFR GCN whereas it was 2.9 and 3.1 months in those with low FISH and CISH EGFR GCN (p = 0.04 and 0.02 respectively). FISH and CISH EGFR GCN may both represent effective tools for a further patients selection in K-RAS wild-type colorectal cancer treated with cetuximab

  13. Precise measurement of the absolute fluorescence yield of nitrogen in air. Consequences on the detection of ultra-high energy cosmic rays; Mesure precise du rendement absolu de la fluorescence de l'azote dans l'air. Consequences sur la detection des rayons cosmiques d'ultra-haute energie

    Energy Technology Data Exchange (ETDEWEB)

    Lefeuvre, G

    2006-07-15

    The study of the energy spectrum of ultra-high energy cosmic rays (E > 10{sup 20} eV) requires to determine the energy with much more precision than what is currently achieved. The shower of particles created in the atmosphere can be detected either by sampling particle on the ground, or by detecting the fluorescence induced by the excitation of nitrogen by shower electrons. At present, the measurement of the fluorescence is the simplest and the most reliable method, since it does not call upon hadronic physics laws at extreme energies, a field still inaccessible to accelerators. The precise knowledge of the conversion factor between deposited energy and the number of fluorescence photons produced (the yield) is thus essential. Up to now, it has been determined with an accuracy of 15 % only. This main goal of this work is to measure this yield to better than 5 per cent. To do this, 1 MeV electrons from a radioactive source excite nitrogen of the air. The accuracy has been reached thanks to the implementation of a new method for the absolute calibration of the photomultipliers detecting the photons, to better than 2 per cent. The fluorescence yield, measured and normalized to 0.85 MeV, 760 mmHg and 15 Celsius degrees, is (4.23 {+-} 0.20) photons per meter, or (20.46 {+-} 0.98) photons per deposited MeV. In addition, and for the first time, the absolute fluorescence spectrum of nitrogen excited by a source has been measured with an optical grating spectrometer. (author)

  14. Fish assemblages

    Science.gov (United States)

    McGarvey, Daniel J.; Falke, Jeffrey A.; Li, Hiram W.; Li, Judith; Hauer, F. Richard; Lamberti, G.A.

    2017-01-01

    Methods to sample fishes in stream ecosystems and to analyze the raw data, focusing primarily on assemblage-level (all fish species combined) analyses, are presented in this chapter. We begin with guidance on sample site selection, permitting for fish collection, and information-gathering steps to be completed prior to conducting fieldwork. Basic sampling methods (visual surveying, electrofishing, and seining) are presented with specific instructions for estimating population sizes via visual, capture-recapture, and depletion surveys, in addition to new guidance on environmental DNA (eDNA) methods. Steps to process fish specimens in the field including the use of anesthesia and preservation of whole specimens or tissue samples (for genetic or stable isotope analysis) are also presented. Data analysis methods include characterization of size-structure within populations, estimation of species richness and diversity, and application of fish functional traits. We conclude with three advanced topics in assemblage-level analysis: multidimensional scaling (MDS), ecological networks, and loop analysis.

  15. HUBUNGAN KECUKUPAN ASAM EIKOSAPENTANOAT (EPA, ASAM DOKOSAHEKSANOAT (DHA IKAN DAN STATUS GIZI DENGAN PRESTASI BELAJAR SISWA

    Directory of Open Access Journals (Sweden)

    Siti Zulaihah

    2012-04-01

    Full Text Available ABSTRACT   Background: Fish contain of high protein, EPA, DHA needed for the formation of brain cell and improving intelligence. Consuming fish and other sea food make healthy and improve the brain ability to reach optimum study achievement. In 2003, fish consumption in Indonesia is still low 24,67kg/capita/year. Based on BPS 2002, fish consumption in Semarang is 5,38%. The fish consumption has a big influence on nutrition sufficiency especially EPA and DHA, nutrition status and attaining healthy and smart Indonesian human resources. Goal: To analyze the relationship between fish meal frequency, fish EPA and DHA recommended and nutrition status with student's study achievement. Method: The research used survey method, analytical research, and cross -sectional time approach. This research was conducted on September-October 2004. Sample was 100 subject of SD Taqwiyatui Wathon (grade IV are 54 person, grade V are 46 person by using Stratified Random Sampling method. The data preparation used NUTRISOFT. Result: Fish frequently consumed by responden was bandeng (Chanos chanos 5%, tongkol (Euthynnus allitteratus rafmescue 4%, kembung (Scomber kanoguria russei 1% and mujair (Tilapia mossambica 1 %. EPA, DHA % RDA defisit 62%, normal nutritional status 93% and average category of study achievement 55%. There was relation between fish meal frequency and fish EPA, DHA % RDA (ρ=0,000, there was no relation between fish meal frequency and nutritional status (ρ=0,213, there was relation between fish meal frequency and study achievement (ρ=0,000, there was relation between fish EPA, DHA recommendation and study achievement (ρ=0,000, and there was no relation between nutrition status and study achievement (ρ=0.378. Based on Pearson correlation test, there was no relation between fish EPA, DHA recommendation and nutritional status (ρ=0,000. Conclution: Students with frequent fish consumption and high RDA of EPA, DHA % RDA showed better study achievement

  16. Epidermal Growth Factor Receptor (EGFR gene copy number (GCN correlates with clinical activity of irinotecan-cetuximab in K-RAS wild-type colorectal cancer: a fluorescence in situ (FISH and chromogenic in situ hybridization (CISH analysis

    Directory of Open Access Journals (Sweden)

    Scartozzi Mario

    2009-08-01

    Full Text Available Abstract Background K-RAS wild type colorectal tumors show an improved response rate to anti-EGFR monoclonal antibodies. Nevertheless 70% to 40% of these patients still does not seem to benefit from this therapeutic approach. FISH EGFR GCN has been previously demonstrated to correlate with clinical outcome of colorectal cancer treated with anti-EGFR monoclonal antibodies. CISH also seemed able to provide accurate EGFR GCN information with the advantage of a simpler and reproducible technique involving immunohistochemistry and light microscopy. Based on these findings we investigated the correlation between both FISH and CISH EGFR GCN and clinical outcome in K-RAS wild-type colorectal cancer treated with irinotecan-cetuximab. Methods Patients with advanced K-RAS wild-type, colorectal cancer receiving irinotecan-cetuximab after failure of irinotecan-based chemotherapy were eligible. A cut-off value for EGFR GCN of 2.6 and 2.12 for FISH and CISH respectively was derived from ROC curve analysis. Results Forty-four patients were available for analysis. We observed a partial remission in 9 (60% and 2 (9% cases with a FISH EGFR GCN ≥ 2.6 and Conclusion FISH and CISH EGFR GCN may both represent effective tools for a further patients selection in K-RAS wild-type colorectal cancer treated with cetuximab.

  17. [Progress in transgenic fish techniques and application].

    Science.gov (United States)

    Ye, Xing; Tian, Yuan-Yuan; Gao, Feng-Ying

    2011-05-01

    Transgenic technique provides a new way for fish breeding. Stable lines of growth hormone gene transfer carps, salmon and tilapia, as well as fluorescence protein gene transfer zebra fish and white cloud mountain minnow have been produced. The fast growth characteristic of GH gene transgenic fish will be of great importance to promote aquaculture production and economic efficiency. This paper summarized the progress in transgenic fish research and ecological assessments. Microinjection is still the most common used method, but often resulted in multi-site and multi-copies integration. Co-injection of transposon or meganuclease will greatly improve the efficiency of gene transfer and integration. "All fish" gene or "auto gene" should be considered to produce transgenic fish in order to eliminate misgiving on food safety and to benefit expression of the transferred gene. Environmental risk is the biggest obstacle for transgenic fish to be commercially applied. Data indicates that transgenic fish have inferior fitness compared with the traditional domestic fish. However, be-cause of the genotype-by-environment effects, it is difficult to extrapolate simple phenotypes to the complex ecological interactions that occur in nature based on the ecological consequences of the transgenic fish determined in the laboratory. It is critical to establish highly naturalized environments for acquiring reliable data that can be used to evaluate the environ-mental risk. Efficacious physical and biological containment strategies remain to be crucial approaches to ensure the safe application of transgenic fish technology.

  18. STATUS INTRODUKSI IKAN DAN STRATEGI PELAKSANAAN SECARA BERKELANJUTAN DI PERAIRAN UMUM DARATAN DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Chairulwan Umar

    2013-11-01

    Full Text Available Masuknya jenis ikan asing berpotensi merubah keseimbangan pada perairan umum daratan, dan di Indonesia gejala ini sudah mulai terlihat. Makalah ini bertujuan untuk membahas lebih lanjut tentang perkembangan introduksi ikan asing, strategi pelaksanaan intoduksi dan berbagai dampaknya terhadap komunitas ikan asli perairan di Indonesia. Penelitian dilakukan melalui penelusuran dan studi pustaka tentang: sejarah kegiatan introduksi ikan yang dilakukan ke Indonesia, jenis-ikan introduksi ikan di Indonesia dan kegiatan introduksi ikan yang telah dan dilakukan di Indonesia beserta dampaknya. Verifikasi lapangan dilakukan pada tahun 2011 di beberapa danau dan waduk antara lain Danau Sentani, Danau Kerinci, Danau Matano dan Waduk Riam Kanan. Data yang terkumpul ditabulasi dan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa introduksi ikan asing dilakukan di Indonesia sebelum tahun 1900. Introduksi ikan yang telah dilakukan selama ini, lebih banyak dilakukan tanpa melalui kajian ilmiah yang mendalam dan telah terbukti mengakibatkan hilang atau berkurangnya populasi ikan asli atau endemik serta menjadi agen pembawa penyakit. The entry of alien fish species could potentially change the balance of the inland waters and in Indonesia this symptoms was shown. The paper aims are to discuss more about the development introduction of alien fish species, the introduction implementation strategy and its impact to indigenous fish species communities in Indonesian inland waters. The study was conducted through a literature research about: the history of fish introductions activities which carried out in Indonesia, the species of fish introduction in Indonesia and the activities of introduction fish that have been conducted in Indonesia and its impact. Field verification was conducted in 2011 at several lakes and reservoir such us: Sentani Lake, Kerinci Lake, Matano Lake and Riam Kanan Reservoir. The collected data were tabulated and analyzed

  19. Epidermal Growth Factor Receptor (EGFR) gene copy number (GCN) correlates with clinical activity of irinotecan-cetuximab in K-RAS wild-type colorectal cancer: a fluorescence in situ (FISH) and chromogenic in situ hybridization (CISH) analysis.

    Science.gov (United States)

    Scartozzi, Mario; Bearzi, Italo; Mandolesi, Alessandra; Pierantoni, Chiara; Loupakis, Fotios; Zaniboni, Alberto; Negri, Francesca; Quadri, Antonello; Zorzi, Fausto; Galizia, Eva; Berardi, Rossana; Biscotti, Tommasina; Labianca, Roberto; Masi, Gianluca; Falcone, Alfredo; Cascinu, Stefano

    2009-08-27

    K-RAS wild type colorectal tumors show an improved response rate to anti-EGFR monoclonal antibodies. Nevertheless 70% to 40% of these patients still does not seem to benefit from this therapeutic approach. FISH EGFR GCN has been previously demonstrated to correlate with clinical outcome of colorectal cancer treated with anti-EGFR monoclonal antibodies. CISH also seemed able to provide accurate EGFR GCN information with the advantage of a simpler and reproducible technique involving immunohistochemistry and light microscopy. Based on these findings we investigated the correlation between both FISH and CISH EGFR GCN and clinical outcome in K-RAS wild-type colorectal cancer treated with irinotecan-cetuximab. Patients with advanced K-RAS wild-type, colorectal cancer receiving irinotecan-cetuximab after failure of irinotecan-based chemotherapy were eligible. A cut-off value for EGFR GCN of 2.6 and 2.12 for FISH and CISH respectively was derived from ROC curve analysis. Forty-four patients were available for analysis. We observed a partial remission in 9 (60%) and 2 (9%) cases with a FISH EGFR GCN >or= 2.6 and CISH EGFR GCN >or= 2.12 and CISH EGFR GCN whereas it was 2.9 and 3.1 months in those with low FISH and CISH EGFR GCN (p = 0.04 and 0.02 respectively). FISH and CISH EGFR GCN may both represent effective tools for a further patients selection in K-RAS wild-type colorectal cancer treated with cetuximab.

  20. Keragaman dan Peran Biologi Arthrophoda pada Sawah Irigasi dan Tegalan

    OpenAIRE

    Suwarno, Suwarno

    2016-01-01

    Penelitian ini bertujuan untuk mengetahui keragaman arthropoda dan peran biologinya pada tanah sawah irigasi dan tegalan. Penelitian ini dilaksanakan di daerah persawahan di daerah Sragen pada bulan Maret – Mei 2016. Metode Penelitian dengan menggunakan pitfall trap atau perangkap jebakan yang diletakkan area persawahan dan tegalan. Teknik pengumpulan data dengan menggunakan purposive sampling. Sampel arthropoda yang diperoleh diidentifikasi dan dianalisis di Laboratorium pendidikan Biologi. ...

  1. One Fish, Two Fish, Redfish, You Fish!

    Science.gov (United States)

    White, Katherine; Timmons, Maryellen; Medders, Paul

    2011-01-01

    The recreational fishing activity presented in this article provides a hands-on, problem-based experience for students; it unites biology, math, economics, environmental policy, and population dynamics concepts. In addition, the activity allows students to shape environmental policy in a realistic setting and evaluate their peers' work. By…

  2. Evaluasi Sifat Morpologi dan Teknologi Pengolahan pada Gabah dan Beras

    OpenAIRE

    Santosa, Susilo

    2017-01-01

    Penelitian evaluasi dan beras terhadap mutu dan jumlah hasil ditekankan pada lr36 , lr38 dan Cisadane. Penelitian dilakukan di laboratorium Balai Penelitian Tanaman Pangan Karawang. Hasil studi menunjukkan diantara ketiga varitas, Cisadane mempunyai ukuran dimensi butiran yang lebih besar daripada Ir36 dan Ir38. Di antara ketiga varitas, persen kulit sekam yang tertinggi didapat 23,57 % pada lr38 diikuti 22,32% pada Ir36 dan 19,5% pada Cisadane. Persen beras pecah kulit tertinggi diperoleh pa...

  3. Evaluasi Sifat Morpologi Dan Teknologi Pengolahan Pada Gabah Dan Beras

    OpenAIRE

    Santosa, Susilo

    1982-01-01

    Penelitian evaluasi dan beras terhadap mutu dan jumlah hasil ditekankan pada lr36 , lr38 dan Cisadane. Penelitian dilakukan di laboratorium Balai Penelitian Tanaman Pangan Karawang. Hasil studi menunjukkan diantara ketiga varitas, Cisadane mempunyai ukuran dimensi butiran yang lebih besar daripada Ir36 dan Ir38. Di antara ketiga varitas, persen kulit sekam yang tertinggi didapat 23,57 % pada lr38 diikuti 22,32% pada Ir36 dan 19,5% pada Cisadane. Persen beras pecah kulit tertinggi diperoleh pa...

  4. Pengaruh Jenis Tepung dan Pengolahan Ikan Terhadap Mutu Tepung Sala Lauk

    Directory of Open Access Journals (Sweden)

    Kamsina Kamsina

    2011-12-01

    Full Text Available Sala lauk is a specific food of Pariaman City of West Sumatera which is made from rice flour, fish, chilli and other spices, then mixed together, cooked with low heat until smooth. The dough was formed into small balls and then fried. It is now available sala lauk flour mixed with spices, but chilli and fish are not included in the mixture and the flour still has short shelf life. The research was conducted to enrich the nutrients in the sala lauk by adding fish as a protein source. Completely randomized design (CRD in factorial was used to see the effect of the use of flour, rice flour 100%, rice flour 95% and glutinous rice flour 5%, rice flour 95% and tapioca flour 5% and the variation of fish treatment that was directly dried, cooked in a pressure cooker and steamed. The result showed that sala lauk flour using rice flour 95% and glutinous rice flour 5% with directly dried fish gave preferred result in terms of organoleptic (taste, colour, flavour, and texture, with water content was 8.97%, acid degree was 0.35%, and protein content was 71.31%. Mold/yeast, salmonella and other bacteria were not found and the product had shelf life for 3 months.ABSTRAKSala lauk merupakan makanan khas Pariaman Sumatera Barat yang terbuat dari bahan baku tepung beras, ikan, cabe, dan bumbu lainnya dengan cara diadon, dimasak dengan api kecil sampai kalis. Adonan dibentuk menjadi bulat-bulat kecil dan kemudian digoreng. Saat ini sudah tersedia tepung sala lauk yang dicampur langsung dengan bumbubumbu, tetapi cabe dan ikan belum termasuk dalam campuran, dan tepung tersebut masih memiliki daya tahan simpan yang masih rendah. Penelitian dilakukan untuk memperkaya nutrisi dalam sala lauk dengan menambahkan sumber protein yaitu ikan. Rancangan acak lengkap (RAL secara faktorial digunakan untuk melihat pengaruh tiga perlakuan pemakaian tepung yaitu tepung beras 100%, tepung beras 95% dan tepung beras ketan 5%, tepung beras 95% dan tepung tapioka 5% dan variasi

  5. Alabama ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, and freshwater fish species in Alabama. Vector polygons in this data set represent...

  6. Maryland ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and freshwater fish species in Maryland. Vector polygons in this data...

  7. Hawaii ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for reef, marine, estuarine, and native stream fish species in coastal Hawaii. Vector polygons in this data...

  8. Virginia ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and brackishwater fish species in Virginia. Vector polygons in this data...

  9. Louisiana ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for freshwater (inland) fish species in coastal Louisiana. Vector polygons represent water-bodies and other...

  10. Adapting Fishing Policy to Climate Change with the Aid of Scientific ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Climate change affects the fishing sector in various ways, by modifying the composition of animal and plant populations, ocean currents, and the frequency and intensity of tropical storms. Over-fishing ... Coopération entre milieux ruraux et urbains dans la gestion de l'eau face aux changements climatiques au Burkina Faso.

  11. Fishing Access Areas

    Data.gov (United States)

    Vermont Center for Geographic Information — The Vermont Fish & Wildlife Department maintains developed fishing access areas. These sites provide public access to waters in Vermont for shore fishing...

  12. Liever kraanwater dan bronwater

    NARCIS (Netherlands)

    Kole, A.P.W.

    2011-01-01

    Een test in het Restaurant van de Toekomst van de invloed van CO2-labels op het aankoopgedrag van consumenten, heeft nog geen duidelijk beeld opgeleverd. Wel pakten mensen vaker kraanwater dan bronwater.

  13. Dan Performer Mei Lanfang

    DEFF Research Database (Denmark)

    Risum, Janne

    2010-01-01

    The convention of performing female characters (dan characters) in Beijing opera, as practised by its most prominent male performer of female characters Mei Lanfang, and its and his cultural context and aesthetic aim...

  14. Do bacteria, not fish, produce 'fish kairomone'?

    NARCIS (Netherlands)

    Ringelberg, J.; Van Gool, E.

    1998-01-01

    Fish-associated chemicals enhance phototactic downward swimming in Daphnia. If perch were treated with the antibiotic ampicillin, this enhancement was significantly decreased. Therefore, not fish, but bacteria associated with fish, seem to produce this kairomone. [KEYWORDS: Diel vertical migration;

  15. Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).

    Science.gov (United States)

    Hwang, Harry C; Gown, Allen M

    2016-01-01

    Current standard of care requires that HER2 gene testing be performed on all newly diagnosed invasive breast cancers in order to determine eligibility for anti-HER2 antibody therapy and should be performed in accordance with current ASCO-CAP guidelines (Hammond et al., J Clin Oncol 29(15):e458, 2011; Wolff et al., J Clin Oncol 31(31):3997-4013, 2013). Here we describe a HER2 FISH methodology to evaluate HER2 gene status in FFPE breast tumor specimens.

  16. TINGKAT EFISIENSI PRODUKSI DAN PENDAPATAN PADA USAHA PENGOLAHAN IKAN ASIN SKALA KECIL

    Directory of Open Access Journals (Sweden)

    Himawan Arif Sutanto

    2015-08-01

    Full Text Available The aim of this research was to measure the level of production efficiency and calculate the return and cost salted fish processing small scale in Pekalongan municipality. There are 20 business owners of small scale processing of salted fish were sampled with snowball sampling. Stochastic Frontier Analysis is used to measure the level of efficiency and descriptive statistics are used to calculate the ratio of return and cost. The results indicate that the level of efficiency of production of salted fish processing small scale in Pekalongan yet efficient so it is still possible to be improved. Factors that influence the production of salted fish in Pekalongan is availability of fish, labor, equipment or facilities, auxiliary materials and extensive effort. Salted fish processing businesses in Pekalongan small scale is still quite favorable, as indicated by the R/C value of 1.37 which indicates that the amount of revenue entrepreneurs of small-scale fish processing is still greater than the costs to be incurred in running the business.Penelitian ini bertujuan untuk mengukur tingkat efisiensi produksi dan menghitung rasio biaya dan pendapatan usaha pengolahan ikan asin skala kecil di kota pekalongan. Sampel dalam penelitian ini adalaah 20 pemilik usaha ikan asin dengan metode snowball sampling. Analisis frontier stokastik digunakan untuk mengukur tingkat efisiensi dan metode deskriptif statistik digunakan untuk menghitung rasio biaya dan pendapatan. Hasil penelitian ini menunjukan bahwa usaha ikan asin skala kecil di kota pekalongan telah cukup efisien sehingga masih memungkinkan untuk ditingkatkan.  Faktor yang mempengaruhi efisiensi usaha ikan asin di kota pekalongan adalah ketersediaan bahan baku ikan, tenaga kerja, peralatan usaha, bahan penolong, dan luas usaha. Usaha pengolahan ikan asin di pekalongan masih menguntungkan, hal ini dapat terlihat dari nilau R/C sebesar 1,37 yang mengindikasikan keuntungan usaha masih lebih tinggi dibandingkan

  17. Fish irradiation

    International Nuclear Information System (INIS)

    Kovacs, J.; Tengumnuay, C.; Juangbhanich, C.

    1970-01-01

    Chub-mackerel was chosen for the study because they are the most common fish in Thailand. Preliminary investigations were conducted to determine the maximum radiation dose of gamma-rays by organoleptic tests. The samples were subjected to radiation at various doses up to 4 Mrad. Many experiments were conducted using other kinds of fish. The results showed that 1 Mrad would be the maximum acceptable dose for fish. Later, the influence of the radiation dose from 0.1-1 Mrad was studied in order to find the optimum acceptable dose for preservation of fish without off-flavour. For this purpose, the Hedonic scale was used. It was found that 0.2 and 0.5 Mrad gave the best result on Chub mackerel. The determinations of optimum dose, organoleptic, microbiological and trimethylamine content changes were done. The results showed that Chub mackerel irradiated at 0.2, 0.5 and 1 Mrad stored at 3 0 C for 71 days were still acceptable, on the contrary the untreated samples were found unacceptable at 14 days. The trimethylamine increment was significantly higher in the untreated samples. At 15 days storage, trimethylamine in the non-irradiated Chub-mackerel was about 10 times higher than the irradiated ones. At 51 and 79 days storage, about 13 times higher in the control samples than the irradiated samples except 0.1 Mrad. Only 2 times higher was found for the 0.1 Mrad. The microbiological results showed that the irradiation above 0.2 Mrad gave favorable extension of shelf-life of fish

  18. EVALUASI VARIASI FENOTIPE DAN GENOTIPE POPULASI IKAN TAMBAKAN DARI JAWA BARAT, KALIMANTAN TENGAH, DAN JAMBI DENGAN TRUSS MORFOMETRIK DAN RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD

    Directory of Open Access Journals (Sweden)

    Anang Hari Kristanto

    2018-01-01

    Full Text Available Ikan tambakan (Helostoma temminckii digemari sebagai ikan konsumsi, di daerah Sumatera dan Kalimantan. Dalam rangka pengembangan budidayanya melalui program domestikasi, informasi terkait variasi fenotipe dan genotipe induk asal perlu dilakukan. Penelitian ini bertujuan untuk mendapatkan karakteristik fenotipe dan genotipe ikan tambakan dari Jawa Barat, Kalimantan Tengah, dan Jambi. Penelitian dilakukan di Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan (BRPBATPP, Bogor. Data diperoleh melalui pengukuran jarak bagian tubuh berdasarkan metode truss morphometric dan analisis DNA menggunakan metode RAPD. Hasil yang diperoleh menunjukkan bahwa nilai polimorfisme (81,25% dan heterozigositas (0,3544 tertinggi terdapat pada populasi ikan tambakan asal Kalimantan Tengah. Jarak genetik tertinggi antara populasi Jambi dengan Kalimantan Tengah sebesar 0,1452; sedangkan jarak genetik terendah adalah 0,1044 yaitu antara populasi Jambi dengan Jawa Barat. Berdasarkan uji karakter morfometrik diketahui terdapat 13 karakter yang berbeda nyata yaitu A1, A2, A4, A5, B3, C1, C3, C4, C5, D3, D4, D5, dan D6. Populasi Jambi dengan Jawa Barat memiliki hubungan kekerabatan lebih dekat dibanding dengan populasi Kalimantan Tengah. Kissing gouramy (Helostoma temminckii is a favored fish for consumption in Sumatra and Kalimantan area. Currently, information related to phenotypic variation and genotypes of the original broodstocks of kissing gouramy is limited to develop the fish’s culture technology through a domestication program. Therefore, the study was conducted to determine the characteristics of phenotype, and genotype of original kissing gouramy broodstocks. The research was conducted at the Institute for Freshwater Aquaculture Research and Development, Bogor. Data collection was carried out by measuring the body length using truss morphometric method and DNA analysis using the RAPD method. The results showed that the highest polymorphism (81

  19. Microbiological spoilage of fish and fish products

    DEFF Research Database (Denmark)

    Gram, Lone; Huss, Hans Henrik

    1996-01-01

    Spoilage of fresh and lightly preserved fish products is caused by microbial action. This paper reviews the current knowledge in terms of the microbiology of fish and fish products with particular emphasis on identification of specific spoilage bacteria and the qualitative and quantitative...... biochemical indicators of spoilage. Shewanzella putrefaciens and Pseudomonas spp. are the specific spoilage bacteria of iced fresh fish regardless of the origin of the fish. Modified atmosphere stored marine fish from temperate waters are spoiled by the CO2 resistant Photobacterium phosphoreum whereas Gram......- positive bacteria are likely spoilers of CO2 packed fish from fresh or tropical waters. Fish products with high salt contents may spoil due to growth of halophilic bacteria (salted fish) or growth of anaerobic bacteria and yeasts (barrel salted fish). Whilst the spoilage of fresh and highly salted fish...

  20. PREFERENSI PEMBIAYAAN USAHA MIKRO DAN KECIL : PERSPEKTIF GENDER DAN ENTREPRENEURABILITY

    Directory of Open Access Journals (Sweden)

    Khaira Amalia Fachrudin

    2013-06-01

    Full Text Available Preferensi pembiayaan dalam perusahaan besar lebih didasarkan pada karakteristik perusahaan tersebut.  Namun dalam usaha mikro dan kecil hal ini mungkin berbeda dan bergantung pada karakteristik pemiliknya seperti gender dan entrepreneurability.   Perbedaan gender mungkin juga membedakan entrepreneurability dan dukungan yang diterima.  Penelitian ini akan menguji apakah terdapat perbedaan preferensi pembiayaan berdasarkan gender, entrepreneurability berdasarkan gender dan berdasarkan preferensi pembiayaan, serta perbedaan dukungan berdasarkan gender.  Sampel dari usaha mikro dan kecil yang bergerak dalam bidang kuliner  diuji dengan uji beda Independent Sample t Test dan kemudian dengan Crosstabulation untuk lebih memperinci hasilnya.  Hasil uji menunjukkan bahwa tidak terdapat perbedaan yang signifikan preferensi pembiayaan berdasarkan gender dan berdasarkan  entrepreneurability, namun entrepreneurability berbeda signifikan berdasarkan gender dan juga ditemukan bahwa dukungan moral, tenaga, dan modal yang diterima pemilik usaha pria dan wanita tidak berbeda signifikan (p value lebih kecil dari 0.05.   Hasil ini menunjukkan bahwa terdapat kesetaraan gender dalam pembiayaan usaha dan entrepreneurability lebih tinggi pada pria.  Pria pemilik usaha juga mendapatkan dukungan tenaga kerja yang lebih banyak daripada wanita.  Dukungan tenaga ini adalah salah satu bentuk  financial bootstrapping karena dapat mengurangi pembiayaan usaha

  1. DETEKSI DAN MANAJEMEN REFEEDING SYNDROME

    OpenAIRE

    Darmadi Darmadi; Riska Habriel Ruslie

    2012-01-01

    AbstrakRefeeding syndrome (RFS) dideskripsikan sebagai perubahan biokimiawi, manifestasi klinis dan komplikasi sebagai konsekuensi pemberian nutrisi pada pasien kurang gizi. Refeeding syndrome ini menyebabkan dampak buruk dan kematian. Sindroma ini lebih sering terjadi pada kelompok risiko. Refeeding syndrome merupakan suatu sindroma yang sering tak terdiagnosis oleh karena itu perlu peningkatan pengetahuan dan kesadaran dari tenaga medis untuk mengurangi morbiditas dan mortalitas dari RFS. K...

  2. INOVASI DAN STRATEGI PENCAPAIANNYA

    Directory of Open Access Journals (Sweden)

    Liem Ferryanto

    2009-01-01

    Full Text Available Innovation is the way of life of any institution to profitably sustain its life. It starts with empathy, the ability to reach outside of ourselves and walk in someone else’s shoes, and optimal implementation of the newly advanced technology. Innovation shows its results through continuously hard working efforts known as "10 Thousand Hours Rule". As world uncertainty creates complexity we, instead of predicting, should therefore anticipate the future by creating and managing real options on contingent projects or elements of alternative optimal strategies. This should reflect into our portfolio strategy. Abstract in Bahasa Indonesia: Inovasi merupakan darah bagi suatu institusi untuk bisa hidup berkelanjutan serta menguntungkan. Inovasi berupa penemuan baru secara sistematis yang berawal dari empati, kemampuan untuk melihat dunia melalui mata orang lain, dan pemanfaatan secara optimal kemajuan teknologi yang ada. Inovasi baru menghasilkan buahnya melalui kerja keras, yaitu dengan mengikuti “Aturan 10 Ribu Jam” secara berkesinambungan. Ketidakpastian, interaksi, keterbatasan dan degradasi menciptakan kompleksitas tentang kebutuhan dan solusi di masa depan. Oleh sebab itu daripada meramalkan risiko yang bakal terjadi, kita sebaiknya memasang strategi berupa skenario untuk mereduksi akibat dari risiko masa depan yang tidak kita mengerti. Skenario ini dapat diperoleh lewat penciptaan dan penanganan beberapa pilihan nyata atas semua proyek antisipatif yang ada. Kata kunci: Inovasi, ketidakpastian dan kompleksitas, aturan 10 ribu jam, paradoks strategi, peta jalan, empati, kerja berkesinambungan.

  3. Kajian awal kadar merkuri (Hg dalam ikan dan kerang di Teluk Kao, Pulau Halmahera

    Directory of Open Access Journals (Sweden)

    Edward Edward

    2017-09-01

    Full Text Available The aim of the study was to determine the concentration of Hg  in some species of fish and mussels harvested from Kao Bay . Fish and mussels samples were purchased from fishermen at Kao Bay  in November 2015. The Hg concentration was measured by using Atomic Absorption Spectrophotometer (AAS.The results showed that the highest concentration of Hg was found in gurara fish (Nemipterus japonicus  that is 0.98 ppm, followed by suo fish  (Sphyraena jello  0.89 ppm,  tatameri fish (Gazza minuta  0.38 ppm, gaca fish (Lutjanus argentimaculatus 0.31 ppm, totodi fish (Synodus foetens  0.24 ppm,  bubara fish (Caranx sp 0, 19 ppm, ngafi fish (Stolephorus indicus 0.19 ppm and biji nangka fish (Upeneus vittatus 0.15 ppm. In the shelfish meat, the highest concentration of Hg is found in the blood mussels (Anadara granosa, that is 0.42 ppm, and then followed by papaco (Telescopium telescopium 0.05 ppm. The concentration of Hg in all samples of fish and shelfish were below from the threshold value for seafood fish and shellfish of 0.5 ppm and 1.0 ppm, respectively. Penelitian ini bertujuan untuk mengetahui kadar Hg dalam beberapa jenis ikan dan kerang yang ada di Teluk Kao dalam kaitannya dengan kesehatan makanan hasil laut. Contoh ikan dan kerang di beli dari nelayan di Teluk Kao pada bulan November 2015. Kadar Hg  diukur dengan menggunakan Spektrofotometer Serapan Atom (AAS. Hasilnya menunjukkan kadar Hg tertinggi dalam ikan dijumpai dalam daging ikan gurara (Nemipterus japonicus yakni 0,98 ppm, selanjutnya diikuti oleh ikan suo (Sphyraena jello 0,89 ppm, ikan tatameri (Gazza minuta 0,38 ppm, ikan gaca (Lutjanus argentimaculatus 0,31 ppm, ikan totodi (Synodus foetens 0,24 ppm, ikan bubara (Caranx sp 0,19 ppm, ikan ngafi (Stolephorus indicus  0,19 ppm, dan ikan biji nangka (Upeneus vittatus 0,15 ppm. Dalam daging kerang kadar Hg tertinggi dijumpai dalam kerang darah (Anadara granosa yakni 0,42 ppm selanjutnya dikuti oleh kerang papaco (Telescopium

  4. Fish gelatin.

    Science.gov (United States)

    Boran, Gokhan; Regenstein, Joe M

    2010-01-01

    Gelatin is a multifunctional ingredient used in foods, pharmaceuticals, cosmetics, and photographic films as a gelling agent, stabilizer, thickener, emulsifier, and film former. As a thermoreversible hydrocolloid with a narrower gap between its melting and gelling temperatures, both of which are below human body temperature, gelatin provides unique advantages over carbohydrate-based gelling agents. Gelatin is mostly produced from pig skin, and cattle hides and bones. Some alternative raw materials have recently gained attention from both researchers and the industry not just because they overcome religious concerns shared by Jews and Muslims but also because they provide, in some cases, technological advantages over mammalian gelatins. Fish skins from a number of fish species are among the other sources that have been comprehensively studied as sources for gelatin production. Fish skins have a significant potential for the production of high-quality gelatin with different melting and gelling temperatures over a much wider range than mammalian gelatins, yet still have a sufficiently high gel strength and viscosity. Gelatin quality is industrially determined by gel strength, viscosity, melting or gelling temperatures, the water content, and microbiological safety. For gelatin manufacturers, yield from a particular raw material is also important. Recent experimental studies have shown that these quality parameters vary greatly depending on the biochemical characteristics of the raw materials, the manufacturing processes applied, and the experimental settings used for quality control tests. In this review, the gelatin quality achieved from different fish species is reviewed along with the experimental procedures used to determine gelatin quality. In addition, the chemical structure of collagen and gelatin, the collagen-gelatin conversion, the gelation process, and the gelatin market are discussed. Copyright © 2010 Elsevier Inc. All rights reserved.

  5. Fish Immunoglobulins

    OpenAIRE

    Sara Mashoof; Michael F. Criscitiello

    2016-01-01

    The B cell receptor and secreted antibody are at the nexus of humoral adaptive immunity. In this review, we summarize what is known of the immunoglobulin genes of jawed cartilaginous and bony fishes. We focus on what has been learned from genomic or cDNA sequence data, but where appropriate draw upon protein, immunization, affinity and structural studies. Work from major aquatic model organisms and less studied comparative species are both included to define what is the rule for an immunoglob...

  6. Fish cognition

    OpenAIRE

    Bshary, Redouan; Brown, Culum

    2017-01-01

    The central nervous system, and the brain in particular, is one of the most remarkable products of evolution. This system allows an individual to acquire, process, store and act on information gathered from the environment. The resulting flexibility in behavior beyond genetically coded strategies is a prime adaptation in animals. The field of animal cognition examines the underlying processes and mechanisms. Fishes are a particularly interesting group of vertebrates to study cognition for two...

  7. Fish hemoglobins

    OpenAIRE

    Souza,P.C. de; Bonilla-Rodriguez,G.O.

    2007-01-01

    Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta) and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemica...

  8. PENGELOLAAN PENELITIAN DAN PENGEMBANGAN KESEHATAN DI THAILAND, MYANMAR, DAN INDIA

    Directory of Open Access Journals (Sweden)

    Anorital, SKM Anorital, SKM

    2012-09-01

    Full Text Available Dalam bulan Januari 1991 yang lalu, Sdr. Anorital, SKM (Ka. Subbag. Pengumpulan dan PengolahanData Badan Litbangkes dan H. Syafwani Mirin, SKM (Ka. Bag. Keuangan Badan Litbangkes memperoleh fellowship dari WHO untuk melakukan studi perbandingan ke institusi-institusi penelitian kesehatan di Thailand,Myanmar, dan India.Berikut di bawah ini tulisan bersangkutan yang menggambarkan secara garis besar pengelolaan penelitian dan pengembangan kesehatan pada masing-masing negara obyek studi. Semoga informasi yang terkandung pada tulisan ini dapat bermanfaat bagi pengembangan Badan Penelitian dan Pengembangan Kesehatan.

  9. Fish hemoglobins

    Directory of Open Access Journals (Sweden)

    P.C. de Souza

    2007-06-01

    Full Text Available Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemical strategies to adapt to the changing environmental gas availability. Structurally, most fish hemoglobins are tetrameric; however, those from some species such as lamprey and hagfish dissociate, being monomeric when oxygenated and oligomeric when deoxygenated. Fish blood frequently possesses several hemoglobins; the primary origin of this finding lies in the polymorphism that occurs in the globin loci, an aspect that may occasionally confer advantages to its carriers or even be a harmless evolutionary remnant. On the other hand, the functional properties exhibit different behaviors, ranging from a total absence of responses to allosteric regulation to drastic ones, such as the Root effect.

  10. Kinerja Dan Efisiensi Bank Pemerintah (Bumn) Dan Busn Yang Go Publik Di Indonesia

    OpenAIRE

    Haryanto, Sugeng

    2012-01-01

    Penelitian ini menganalisis kinerja dan tingkat efisiensi bank-bank BUMN dan BUSN yang go Publik di Bursa Efek Indonesia. Sample penelitian ini mengambil tiga bank BUMN Bank BNI 46, Bank Mandiri dan Bank BRI) dan tiga bank BUSN (Bank BCA, Bank Niaga dan Bank Panin) dengan periode analisis tahun 2005-2011. Varibael yang digunakan meliputi ROA, ROE, LAR. LDR, NPL dan BOPO. Tujuan dari penelitian ini adalah melihat dan menganalisis perbedaan kinerja antara Bank BUMN dan BUSN yang go public di...

  11. Kondisi oral higiene dan karies gigi pada vegetarian dan non vegetarian di Maha Vihara Maitreya Medan

    OpenAIRE

    Prawira, Albert

    2011-01-01

    Perbedaan pola makan antara vegetarian dan non vegetarian dapat mempengaruhi kesehatan gigi dan mulut. Tujuan penelitian adalah untuk mengetahui kondisi oral higiene dan karies gigi pada umat vegetarian dan non vegetarian di Maha Vihara Maitreya Medan. Jenis penelitian adalah survei deskriptif. Sampel terdiri atas 74 vegetarian dan 65 non vegetarian. Pemeriksaan oral higiene dan karies gigi masing-masing menggunakan indeks OHIS Greene dan Vermillion dan indeks DMFT Klein, se...

  12. Fish Tales

    International Nuclear Information System (INIS)

    McLerran, L.

    2010-01-01

    This talk is about fishing and the friendships that have resulted in its pursuit. It is also about theoretical physics, and the relationship of imagination and fantasy to the establishment of ideas about nature. Fishermen, like theoretical physicists, are well known for their inventive imaginations. Perhaps neither are as clever as sailors, who conceived of the mermaid. If one doubts the power of this fantasy, one should remember the ghosts of the many sailors who drowned pursuing these young nymphs. An extraordinary painting by J. Waterhouse is shown as Fig. 1. The enchantment of a mermaid must reflect an extraordinary excess of imagination on the part of the sailor, perhaps together with an impractical turn of mind. A consummated relationship with a mermaid is after all, by its very nature a fantasy incapable of realization. To a theoretical physicist, she is symbolic of many ideas we develop. There are many truths known to fisherman in which one might also find parallels to the goals of scientists: (1) A fish is the only animal that keeps growing after its death; (2) Nothing makes a fish bigger than almost being caught; (3) ''...of all the liars among mankind, the fisherman is the most trustworthy.'' (William Sherwood Fox, in Silken Lines and Silver Hooks); and (4) Men and fish are alike. They both get into trouble when they open their mouths. These quotes may be interpreted as reflecting skepticism regarding the honesty of fisherman, and probably do not reflect adequate admiration for a creative imagination. Is it fair to criticize a person for believing a falsehood that he or she sincerely believes to be true? The fisherman simultaneously invents the lie, and believes in it himself. The parallel with theoretical physics is perhaps only approximate, although we physicists may invent stories that we come to believe, on some rare occasions our ideas actually correspond to a more or less true descriptions of nature. These minor philosophical differences are not

  13. Fish Tales

    Energy Technology Data Exchange (ETDEWEB)

    McLerran, L.

    2010-07-06

    This talk is about fishing and the friendships that have resulted in its pursuit. It is also about theoretical physics, and the relationship of imagination and fantasy to the establishment of ideas about nature. Fishermen, like theoretical physicists, are well known for their inventive imaginations. Perhaps neither are as clever as sailors, who conceived of the mermaid. If one doubts the power of this fantasy, one should remember the ghosts of the many sailors who drowned pursuing these young nymphs. An extraordinary painting by J. Waterhouse is shown as Fig. 1. The enchantment of a mermaid must reflect an extraordinary excess of imagination on the part of the sailor, perhaps together with an impractical turn of mind. A consummated relationship with a mermaid is after all, by its very nature a fantasy incapable of realization. To a theoretical physicist, she is symbolic of many ideas we develop. There are many truths known to fisherman in which one might also find parallels to the goals of scientists: (1) A fish is the only animal that keeps growing after its death; (2) Nothing makes a fish bigger than almost being caught; (3) ''...of all the liars among mankind, the fisherman is the most trustworthy.'' (William Sherwood Fox, in Silken Lines and Silver Hooks); and (4) Men and fish are alike. They both get into trouble when they open their mouths. These quotes may be interpreted as reflecting skepticism regarding the honesty of fisherman, and probably do not reflect adequate admiration for a creative imagination. Is it fair to criticize a person for believing a falsehood that he or she sincerely believes to be true? The fisherman simultaneously invents the lie, and believes in it himself. The parallel with theoretical physics is perhaps only approximate, although we physicists may invent stories that we come to believe, on some rare occasions our ideas actually correspond to a more or less true descriptions of nature. These minor philosophical

  14. An Assessment of the Heavy Metal Content of Cat Fish (Clarias Lazera) obtained from the Lower Niger Basin at Idah, Kogi State using Energy Dispersive X- ray Fluorescence (EDXRF) Technique

    International Nuclear Information System (INIS)

    Amodu, A.E.; Achumu, L. A.; Egwuogu, C.P.

    2013-01-01

    Five catfish (Clarias lazera) samples obtained from the Lower Niger River Basin at Idah were subjected to elemental analysis by Energy Dispersive X - ray Fluorescence (EDXR) technique. A voltage of 30KV and current of 1mA was applied to produce a 17.441KeV molybdenum X-ray which was used to irradiate the samples for ten minutes. The analysis was performed using the mini pal 4 version PW 4030 X -ray spectrometer at the Center for Energy Research and Training (CERT), Ahmadu Bello University, Zaria, Kaduna State, Nigeria. The elemental composition and concentration of seven major and trace elements: Ca, Cr, K, Cu, Fe, Mn, and V were detected in the samples. The major elements are Ca,K and Fe while the trace elements are Cr,Cu, V and Mn. The concentration of Calcium which is the highest range fro 0.370% to 3.110% while the concentration of Copper which is the least range from 46.030pm to 99.859pm. The result shows the presence of Cr, Cu, Fe, Mn and V which are heavy metals the concentration of Cr, Cu, Fe and Mn in the sample were below World Health Organization (WHO) and Food and Agricultural Organization(FAO) maximum permissible limit of intake of the various minerals respectively. However the concentration of V found in the sample is above the WHO and FAO maximum limit for Vanadium.

  15. OPTIMASI JUMLAH RUMPON, UNIT ARMADA DAN MUSIM PENANGKAPAN PERIKANAN TUNA DI PERAIRAN PRIGI, JAWA TIMUR

    Directory of Open Access Journals (Sweden)

    Erfind Nurdin

    2016-05-01

    Full Text Available Sebagai alat bantu penangkapan ikan, rumpon berfungsi untuk menarik kelompok ikan agar berkumpul di sekitarnya. Dalam jangka pendek rumpon dapat meningkatkan produksi hasil tangkapan, efisiensi dan efektivitas operasi penangkapan ikan. Namun rumpon juga dapat berdampak negatif terhadap keberlajutan stok sumberdaya. Penelitian ini dilakukan di PPN Prigi, Jawa Timur, dengan tujuan untuk mengkaji status pemanfaatan perikanan tuna, optimasi jumlah unit armada dan rumpon serta musim penangkapan ikan.  Beberapa analisis yang digunakan antara lain linear goal programming (LGP, fishing power indeks (FPI, catch per unit of effort (CPUE, maximum sustainable yield (MSY, dan untuk mengetahui pola musim tangkap menggunakan Metode Persentase Rata-rata. Hasil penelitian menunjukkan terdapat indikasi pemanfaatan perikanan tuna yang berlebih pada tingkat pengupayaan yang melampaui batas maksimum (MSY = 2334,9 ton/tahun.  Jumlah optimum untuk armada jaring insang sebanyak 43 unit, pancing tonda 63 unit dan rumpon 33 unit pada luasan area penelitian 8.940 km². Musim tangkap berlangsung pada Bulan Juni sampai Desember dengan puncak musim di bulan Juli.    Fish Aggregating Device (FADs has a function to attract and aggregate fish schooling. In short term, the advantage of FADs used is to increase the efficiency and effectiveness of fishing operations and the fish caught by the fishers; however FADs might also result a negative impact on the sustainability of fish stock.This study was conducted in fishing area of Prigi National Fishing Port, East Java. The objective of this study is to investigate the tuna fisheries status, optimization number of fishing units and number of FADs. Some analysis methods applied in this study were linear goal programming (LGP, fishing power index (FPI, catch per unit of effort (CPUE, maximum sustainable yield (MSY, and analysis of fishing season using the Average Percentage Methods. The results showed that the tuna fisheries in

  16. Reviews in fluorescence 2010

    CERN Document Server

    Geddes, Chris D

    2011-01-01

    ""Reviews in Fluorescence 2010"", the seventh volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year's progress in fluorescence and its applications, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. ""Reviews in Fluorescence"" offers an essential reference material for any lab working in the fluoresc

  17. Principles of fluorescence techniques

    CERN Document Server

    2016-01-01

    Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

  18. IDE DAN DESAIN

    Directory of Open Access Journals (Sweden)

    Priscilia Yunita Wijaya

    1999-01-01

    Full Text Available We have seen many desain all around our everyday living. Design exist in the form of brochures%2C posters%2C banners%2C signages%2C book covers%2C and etcetera. Some are nice and easy to understand%2C some are ok%2C and some look like they are not fully develop and thought. A word that run across our mind whrn we enjoying a design is idea . A good design almost always associated with a good idea%2C is it tru? This paper is going to discuss about desain and its relationship to idea. Abstract in Bahasa Indonesia : Banyak desain yang diamati dalam kehidupan kita sehari-hari. Desain hadir dalam bentuk brosur%2C poster%2C spanduk%2C tanda-tanda%2C sampul buku%2C dan lain sebagainya. Ada beberapa yang bagus dan mudah untuk dimengerti%2C beberapa yang biasa%2C dan beberapa yang tampak asal dibuat. Sebuah kata yang terlintas dalam pemikiran kita dalam menikmati sebuah desain adalah ide . Sebuah desain yang baik selalu di identikan dengan ide yang baik%2C benarkah hal ini? Apakah desain dan apa hubungannya dengan ide akan dibahas pada tulisan ini.

  19. Fishing activities

    Science.gov (United States)

    Oberle, Ferdinand; Puig, Pere; Martin, Jacobo; Micallef, Aaron; Krastel, Sebastian; Savini, Alessandra

    2018-01-01

    Unlike the major anthropogenic changes that terrestrial and coastal habitats underwent during the last centuries such as deforestation, river engineering, agricultural practices or urbanism, those occurring underwater are veiled from our eyes and have continued nearly unnoticed. Only recent advances in remote sensing and deep marine sampling technologies have revealed the extent and magnitude of the anthropogenic impacts to the seafloor. In particular, bottom trawling, a fishing technique consisting of dragging a net and fishing gear over the seafloor to capture bottom-dwelling living resources has gained attention among the scientific community, policy makers and the general public due to its destructive effects on the seabed. Trawling gear produces acute impacts on biota and the physical substratum of the seafloor by disrupting the sediment column structure, overturning boulders, resuspending sediments and imprinting deep scars on muddy bottoms. Also, the repetitive passage of trawling gear over the same areas creates long-lasting, cumulative impacts that modify the cohesiveness and texture of sediments. It can be asserted nowadays that due to its recurrence, mobility and wide geographical extent, industrial trawling has become a major force driving seafloor change and affecting not only its physical integrity on short spatial scales but also imprinting measurable modifications to the geomorphology of entire continental margins.

  20. Fish tapeworm infection

    Science.gov (United States)

    Fish tapeworm infection is an intestinal infection with a parasite found in fish. ... The fish tapeworm ( Diphyllobothrium latum ) is the largest parasite that infects humans. Humans become infected when they eat raw ...

  1. FISH and PNA FISH for the diagnosis of Q fever endocarditis and vascular infections.

    Science.gov (United States)

    Prudent, Elsa; Lepidi, Hubert; Angelakis, Emmanouil; Raoult, Didier

    2018-06-13

    Purpose. Endocarditis and vascular infections are common manifestations of persistent localized infection due to Coxiella burnetii and recently, fluorescence in situ hybridization (FISH) was proposed as an alternative tool for their diagnosis. In this study, we evaluated the efficiency of FISH in a series of valve and vascular samples infected by C. burnetii. Methods. We tested 23 C. burnetii -positive valves and thrombus samples obtained from patients with Q fever endocarditis. Seven aneurysms and thrombus specimens were retrieved from patients with Q fever vascular infection. Samples were analyzed by culture, immunochemistry and FISH with oligonucleotide and PNA probes targeting C. burnetii -specific 16S ribosomal RNA sequences. Results. Immunohistochemical analysis was positive for five (17%) samples with significantly more copies of C. burnetii DNA than the negative ones ( p= 0.02). FISH was positive for 13 (43%) samples and presented 43% and 40% sensitivity compared to qPCR and culture, respectively. PNA FISH detected C. burnetii in 18 (60%) samples and presented 60% and 55% sensitivity compared to qPCR and culture, respectively. Immunohistochemistry had 38% and 28% sensitivity compared to FISH and PNA FISH, respectively. Samples found positive by both immunohistochemistry and PNA FISH contained significantly more copies of C. burnetii DNA than the negative ones ( p= 0.03). Finally, PNA FISH was more sensitive than FISH (60% versus 43%) for the detection of C. burnetii Conclusion. We provide evidence that PNA FISH and FISH are important assays for the diagnosis of C. burnetii endocarditis and vascular infections. Copyright © 2018 American Society for Microbiology.

  2. Automated Image Analysis for Quantitative Fluorescence In Situ Hybridization with Environmental Samples▿ †

    OpenAIRE

    Zhou, Zhi; Pons, Marie Noëlle; Raskin, Lutgarde; Zilles, Julie L.

    2007-01-01

    When fluorescence in situ hybridization (FISH) analyses are performed with complex environmental samples, difficulties related to the presence of microbial cell aggregates and nonuniform background fluorescence are often encountered. The objective of this study was to develop a robust and automated quantitative FISH method for complex environmental samples, such as manure and soil. The method and duration of sample dispersion were optimized to reduce the interference of cell aggregates. An au...

  3. Reviews in fluorescence 2008

    CERN Document Server

    Geddes, Chris D

    2010-01-01

    This volume serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence spectroscopy. It summarizes the year's progress in fluorescence and its applications as well as includes authoritative analytical reviews.

  4. Fluorescent optical position sensor

    Science.gov (United States)

    Weiss, Jonathan D.

    2005-11-15

    A fluorescent optical position sensor and method of operation. A small excitation source side-pumps a localized region of fluorescence at an unknown position along a fluorescent waveguide. As the fluorescent light travels down the waveguide, the intensity of fluorescent light decreases due to absorption. By measuring with one (or two) photodetectors the attenuated intensity of fluorescent light emitted from one (or both) ends of the waveguide, the position of the excitation source relative to the waveguide can be determined by comparing the measured light intensity to a calibrated response curve or mathematical model. Alternatively, excitation light can be pumped into an end of the waveguide, which generates an exponentially-decaying continuous source of fluorescent light along the length of the waveguide. The position of a photodetector oriented to view the side of the waveguide can be uniquely determined by measuring the intensity of the fluorescent light emitted radially at that location.

  5. Fish welfare: Fish capacity to experience pain

    Directory of Open Access Journals (Sweden)

    Vučinić Marijana

    2009-01-01

    Full Text Available Teleost fish possess similar nociceptive processing systems to those found in terrestrial vertebrates. It means that they react to potential painful stimuli in a similar manner as mammals and birds. However, the welfare of fish has been the focus of less research than that of higher vertebrates. Humans may affect the welfare of fish through fisheries, aquaculture and a number of other activities. There is scientific evidence to support the assumption that fish have the capacity to experience pain because they possess functional nociceptors, endogenous opioids and opioid receptors, brain structures involved in pain processing and pathways leading from nociceptors to higher brain structures. Also, it is well documented that some anaesthetics and analgesics may reduce nociceptive responses in fish. Behavioural indicators in fish such as lip-rubbing and rocking behaviours are the best proof that fish react to potential painful stimuli. This paper is an overview of some scientific evidence on fish capacity to experience pain.

  6. Safe biodegradable fluorescent particles

    Science.gov (United States)

    Martin, Sue I [Berkeley, CA; Fergenson, David P [Alamo, CA; Srivastava, Abneesh [Santa Clara, CA; Bogan, Michael J [Dublin, CA; Riot, Vincent J [Oakland, CA; Frank, Matthias [Oakland, CA

    2010-08-24

    A human-safe fluorescence particle that can be used for fluorescence detection instruments or act as a safe simulant for mimicking the fluorescence properties of microorganisms. The particle comprises a non-biological carrier and natural fluorophores encapsulated in the non-biological carrier. By doping biodegradable-polymer drug delivery microspheres with natural or synthetic fluorophores, the desired fluorescence can be attained or biological organisms can be simulated without the associated risks and logistical difficulties of live microorganisms.

  7. URGENSI DAN PROSPEK PENGATURAN (IUS CONSTITUENDUM UU TENTANG CONTEMPT OF COURT UNTUK MENEGAKKAN MARTABAT DAN WIBAWA PERADILAN

    Directory of Open Access Journals (Sweden)

    Lilik Mulyadi

    2015-07-01

    Full Text Available UU Contempt of Court merupakan kebutuhan yang bersifat urgent, segera dan mendesak, sehingga perlu dilakukan kajian dan penelitian secara kritis, akademis dan bersifat komprehensif untuk menjaga keluhuran dan menegakkan martabat dan wibawa peradilan.

  8. RENDEMEN DAN KOMPOSISI PROKSIMAT GELATIN KULIT IKAN BELUT DAN LELE PADA KEADAAN SEGAR DAN KERING (YIELD AND PROXIMATE OF GELATIN EXTRACTED FROM FRESH AND DRY SWAMP ELL AND CATFISH SKIN

    Directory of Open Access Journals (Sweden)

    Hafni Rahmawati

    2017-02-01

    Full Text Available Kulit ikan belut dan lele berpotensi untuk diekstrak gelatinnya. Kulit ikan belut dan lele tidak bersisik, berlendir dan berlemak untuk ikan lele, berbeda dengan kulit ikan pada umumnya yang dijadikan gelatin. Penelitian ini mempelajari tentang gelatin kulit ikan belut dan lele dari segi rendemen dan komposisi proksimat. Untuk mengetahui pengaruh pengeringan, kondisi kulit segar dan kering juga dipelajari. Tahapan ekstraksi yang dilakukan untuk keseluruhan jenis ikan sama, kecuali penanganan kulit ikan kering yang sebelumnya direndam dalam air selama 4 jam. Kulit ikan direndam kembali dalam 0,05M asam asetat selama 10 jam, kemudian dicuci dan diekstraksi dengan aquadest pada suhu 80OC selama 2 jam, cairan yang didapat difiltrasi. Filtratnya dikeringkan dalam cabinet dryer suhu 55OC selama        48 jam hingga diperoleh lembaran gelatin, kemudian diblender menjadi granula gelatin. Hasil penelitian menunjukkan bahwa gelatin kulit ikan kering mempunyai rendemen sedikit lebih rendah dibandingkan gelatin dari kulit ikan segarnya. Kadar protein gelatin kulit ikan kering telihat tinggi dibandingkan kulit segarnya. Pengeringan kulit ikan berpengaruh pada penurunan kadar abu dan lemak, namun tidak mempengaruhi kadar air gelatin hasil ekstraksi. Gelatin kulit segar ikan lele memiliki nilai rendemen tertinggi yaitu 22,01%. Komposisi proksimat yang terbaik diantara keseluruhan kondisi dan jenis kulit dapat dilihat pada gelatin kulit segar ikan belut dimana memiliki kadar air 9,91%; kadar abu 3,07%; kadar protein 91,61%; dan kadar lemak 0,82%. Skin of swam ell and catfish were potential to gelatin extracted. Swamp ell skin doesn’t have scales, with much mucus and a few fat for catfish, it’s different from another fish skin that gelatin extracted usually. This research was studied yield and proximate gelatin extracted from skin of swam ell and catfish. Influence of drying was observed too. The first stage of the research was raw

  9. Fish for Feed vs Fish for Food

    OpenAIRE

    Allan, Geoff L.

    2004-01-01

    Aquaculture is the fastest-growing food producing industry sector in the world. Demand for feed ingredients, particularly for preferred protein sources such as fishmeal, fish oil and ‘trash fish’, has also increased, raising questions about sustainability and uses of fish for aquaculture feeds or directly as human food. Approximately 30 million metric tonnes (MMT) of fish from capture fisheries are used each year to produce fishmeal and fish oil. The species used are not usually consumed dire...

  10. Optimization of fluorescent proteins

    NARCIS (Netherlands)

    Bindels, D.S.; Goedhart, J.; Hink, M.A.; van Weeren, L.; Joosen, L.; Gadella (jr.), T.W.J.; Engelborghs, Y.; Visser, A.J.W.G.

    2014-01-01

    Nowadays, fluorescent protein (FP) variants have been engineered to fluoresce in all different colors; to display photoswitchable, or photochromic, behavior; or to show yet other beneficial properties that enable or enhance a still growing set of new fluorescence spectroscopy and microcopy

  11. ANALISIS JENIS, JUMLAH, DAN MUTU GIZI KONSUMSI SARAPAN ANAK INDONESIA

    Directory of Open Access Journals (Sweden)

    Fachruddin Perdana

    2013-11-01

    Full Text Available ABSTRACTThe objective of this study was to analyze type, amount, and nutritional quality of breakfast among Indonesian children aged 3—12 years old. Data used for this study was secondary data of Basic Health Survey 2010 (Riskesdas 2010. Data was collected from June until August 2010 by applying a cross sectional design. This study covered 33 provinces of Indonesia, with total 40,437 children consisted of 20,659 boys and 19,778 girls. The results of this study showed that ten major type of foods consumed during breakfast were rice, swamp cabbage, egg, fish, tempe, instant noodles, tofu, bread, chicken and biscuits; and five major type of beverages consumed during breakfast were drinking water, tea, milk, coffee, and syrup. The food consumed on average of more than 5 g a day during breakfast were rice, swamp cabbage, egg, fish, tempe, instant noodles; and the beverages consumed on average of more than 15 mL a day were water, tea, and milk. Only 10.6% of children breakfast met energy intake>30% RDA.Keywords: breakfast, breakfast amount, breakfast type, children, nutritional quality of breakfastABSTRAKTujuan dari penelitian ini adalah untuk menganalisis jenis, jumlah, dan mutu gizi konsumsi sarapan anak Indonesia usia 3—12 tahun. Data yang digunakan untuk penelitian ini adalah data sekunder (data konsumsi pangan yang diperoleh dari hasil penelitian Riset Kesehatan Dasar 2010 (Riskesdas 2010 yang dilaksanakan oleh Badan Penelitian dan Pengembangan Kesehatan, Kementrian Kesehatan Indonesia. Pengumpulan data dilakukan pada bulan Juni sampai Agustus 2010 dengan menggunakan desain cross sectional. Penelitian ini mencakup 33 provinsi di Indonesia, dengan 40 437 total subjek; yang terdiri dari 20 659 laki-laki dan 19 778 perempuan. Hasil penelitian menunjukkan bahwa sepuluh jenis makanan yang paling banyak dikonsumsi selama sarapan adalah nasi, kangkung, telur ayam, ikan, tempe, mi instan, tahu, roti, daging ayam, dan biskuit; lima jenis minuman yang

  12. Epistemologi dan Keterbatasan Teori Gravitasi

    Directory of Open Access Journals (Sweden)

    Erwin Erwin

    2017-08-01

    Full Text Available Peristiwa tentang kecenderungan jatuhnya benda-benda menuju pusat bumi dan keteraturan peredaran planet dan benda-benda langit lainnya dalam tata surya dahulu dianggap dua fenomena yang berbeda. Mekanika benda langit dan mekanika bumi yang sebelumnya merupakan dua pengetahuan yang terpisah, dianggap satu kesatuan oleh Sir Isaac Newton. Newton mengemukakan hukum gravitasi umum yaitu gaya tarik menarik antara dua benda besarnya sebanding dengan massa masing-masing benda dan berbanding terbalik dengan kuadrat jarak antara kedua benda. Hukum gravitasi ini sukses menjalaskan bagaimana benda cendrung jatuh menuju pusat bumi dan peredaran planet dan benda-benda langit lain mengelilingi matahari dalam sistem tata surya. Namun hukum gravitasi Newton ternyata tidak sepenuhnya tepat, beberapa hal dapat dijelaskan dengan hukum relativitas Einstein, namun demikian hukum relativitas Einstein juga dicurigai masih perlu diamandemen agar dapat menjelaskan fenomena alam dengan tepat.

  13. EPISTEMOLOGI DAN KETERBATASAN TEORI GRAVITASI

    Directory of Open Access Journals (Sweden)

    Erwin Erwin

    2017-08-01

    Full Text Available Peristiwa tentang kecenderungan jatuhnya benda-benda menuju pusat bumi dan keteraturan peredaran planet dan benda-benda langit lainnya dalam tata surya dahulu dianggap dua fenomena yang berbeda. Mekanika benda langit dan mekanika bumi yang sebelumnya merupakan dua pengetahuan yang terpisah, dianggap satu kesatuan oleh Sir Isaac Newton. Newton mengemukakan hukum gravitasi umum yaitu gaya tarik menarik antara dua benda besarnya sebanding dengan massa masing-masing benda dan berbanding terbalik dengan kuadrat jarak antara kedua benda. Hukum gravitasi ini sukses menjalaskan bagaimana benda cendrung jatuh menuju pusat bumi dan peredaran planet dan benda-benda langit lain mengelilingi matahari dalam sistem tata surya. Namun hukum gravitasi Newton ternyata tidak sepenuhnya tepat, beberapa hal dapat dijelaskan dengan hukum relativitas Einstein, namun demikian hukum relativitas Einstein juga dicurigai masih perlu diamandemen agar dapat menjelaskan fenomena alam dengan tepat.

  14. Microbiological spoilage of fish and fish products.

    Science.gov (United States)

    Gram, L; Huss, H H

    1996-11-01

    Spoilage of fresh and lightly preserved fish products is caused by microbial action. This paper reviews the current knowledge in terms of the microbiology of fish and fish products with particular emphasis on identification of specific spoilage bacteria and the qualitative and quantitative biochemical indicators of spoilage. Shewanella putrefaciens and Pseudomonas spp. are the specific spoilage bacteria of iced fresh fish regardless of the origin of the fish. Modified atmosphere stored marine fish from temperate waters are spoiled by the CO2 resistant Photobacterium phosphoreum whereas Gram-positive bacteria are likely spoilers of CO2 packed fish from fresh or tropical waters. Fish products with high salt contents may spoil due to growth of halophilic bacteria (salted fish) or growth of anaerobic bacteria and yeasts (barrel salted fish). Whilst the spoilage of fresh and highly salted fish is well understood, much less is known about spoilage of lightly preserved fish products. It is concluded that the spoilage is probably caused by lactic acid bacteria, certain psychotrophic Enterobacteriaceae and/or Photobacterium phosphoreum. However, more work is needed in this area.

  15. Fishing Fish Stem Cells and Nuclear Transplants

    OpenAIRE

    Hong, Yunhan

    2011-01-01

    Fish has been the subject of various research fields, ranging from ecology, evolution, physiology and toxicology to aquaculture. In the past decades fish has attracted considerable attention for functional genomics, cancer biology and developmental genetics, in particular nuclear transfer for understanding of cytoplasmic-nuclear relationship. This special issue reports on recent progress made in fish stem cells and nuclear transfer.

  16. ISLAM DAN RADIKALISME: Upaya Antisipasi dan Penanggulangannya

    Directory of Open Access Journals (Sweden)

    Idrus Ruslan

    2017-02-01

    Full Text Available Sejak tragedi pemboman WTC tanggal 11 September 2001 yang disusul dengan rangkaian peledakan bom di sejumlah negara, banyak orang non Muslim yang berasumsi bahwa ajaran Islam identik dengan radikalisme. Walaupun assumsi yang berkembang itu dapat dimaklumi, namun keyakinan tersebut tentu saja tidak sepenuhnya benar. Karena meskipun terdapat sekelompok orang Islam yang berbuat radikal, akan tetapi mayoritas Muslim justru bertentangan dengan mereka. Lagi pula, jika dicermati secara mendalam, sesungguhnya Islam sama sekali tidak mentolerir tindakan radikal. Ia adalah agama kasih sayang yang sangat mendodrong penganutnya untuk berbuat baik terhadap orang lain, termasuk kepada orang-orang non-Muslim. Artikel ini menawarkan beberapa upaya strategis dalam mengantisipasi dan menanggulangi bahaya radikalisme-terorisme.

  17. DIVERSITAS DAN KERAPATAN MANGROVE, GASTROPODA DAN BIVALVIA DI ESTUARI PERANCAK, BALI

    OpenAIRE

    Susiana; Ali, Syamsu Alam; Rukminasari, Nita

    2011-01-01

    Penelitian ini bertujuan membandingkan diversitas dan kerapatan mangrove dengan kepadatan gastropoda dan bivalvia di mangrove alami dan rehabilitasi. Pengukuran ekosistem mangrove menggunakan transek kuadrat 10 m x 10 m. Kelimpahan dan kepadatan gastropoda dan bialvia menggunakan transek kuadrat berukuran 1 m x 1 m. Analisis nMDS, cluster untuk melihat hubungan karekteristik mangrove alami dan rehabilitasi dianalisis secara deskriptif dan analisis regresi untuk mendetermi...

  18. Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions

    DEFF Research Database (Denmark)

    Jensen, Tim Kåre; Montgomery, Donald L.; Jaeger, Paula T.

    2007-01-01

    A fluorescent in situ hybridisation (FISH) assay targeting 16S ribosomal RNA was developed for detection of the zoonotic bacterium Coxiella burnetii in formalin-fixed, paraffin-embedded tissue, and applied on placentas from ruminant abortions. The applicability of the FISH assay was compared...

  19. HUBUNGAN PANJANG-BERAT DAN FAKTOR KONDISI LOBSTER BATU (Panulirus penicillatus DI PERAIRAN SELATAN GUNUNG KIDUL DAN PACITAN

    Directory of Open Access Journals (Sweden)

    Moh Fauzi Fauzi

    2016-03-01

    Full Text Available Perairan di sebelah selatan Gunung Kidul dan Pacitan merupakan daerah penangkapan lobster yang cukup potensial. Analisis hubungan panjang berat, faktor kondisi dan sebaran kisaran panjang lobster batu (Panulirus penicillatus di perairan tersebut dilakukan pada bulan Maret 2010 hingga Maret 2012. Pengukuran panjang-berat terhadap 1.803 individu lobster batu menunjukkan pola pertumbuhan yang bersifat allometrik negatif. Faktor kondisi lobster memiliki kesamaan antara jenis kelamin jantan dan betina dan terdapat kecenderungan menurunnya faktor kondisi dengan bertambahnya panjang (umur. Panjang karapas dominan berada pada kisaran antara 45-50mm. Lobster betina yang membawa telur ditemukan pertama kali pada kelas panjang karapas antara 35-40mm. The waters in the south of Gunung Kidul and Pacitan were indicated as a potentially fishing ground of spiny lobster. Analysis of Length-weight relationship, condition factor and distribution of the pronghorn spiny lobster (Panulirus penicillatus in those waters were conducted in March 2010 through March 2012. Length-weight measurements on 1.803 individual lobsters showed allometric negative growth patterns. Lobster have in common condition factor between male and female, and there is a tendency condition factor decreased with increasing length (age. Dominant carapace length is in the range between 45-50mm. Female lobsters carrying eggs were first discovered in the class of carapace length between 35-40mm.

  20. DAKWAH DAN PEMBERDAYAAN PEREMPUAN

    Directory of Open Access Journals (Sweden)

    Siti Nurmahyati

    2017-02-01

    Full Text Available Da’wah is an attempt to persuade and influence others in realizing the teachings of Islam, to achieve worldly prosperity and welfare of the hereafter. The success was fueled by propaganda aspects are interrelated, anatara preachers, propaganda material, propaganda strategy, mad’u (target da’wah. The number of women in this country nearly half of people (49.36. therefore, it is important to empower women in da’wah to build their own people in consciousness and religious activities that provide the basics of life prosperous world and hereafter.   Dakwah merupakan suatu upaya untuk mengajak dan mempengaruhi orang lain dalam merealisasikan ajaran Islam, untuk mencapai tujuan kesejahteraan duniawi maupun kesejahteraan ukhrawi. Keberhasilan tersebut pun dipicu oleh berbagai aspek dakwah yang saling terkait, antara juru dakwah, materi dakwah, strategi dakwah, mad’u (sasaran dakwah. Jumlah kaum perempuan di negara ini hampir mencapai separuh dari jumlah masyarakatnya (49,36. Oleh karena itu, sangat penting sekali pemberdayaan perempuan dalam dakwah untuk membangun kaumnya sendiri dalam kesadaran dan aktivitas-aktivitas keagamaan yang memberikan dasar kehidupan sejahtera duniawi dan ukhrawi.

  1. ASIMETRI INFORMASI DAN UNDERPRICING

    Directory of Open Access Journals (Sweden)

    Tety Anggita Safitri

    2013-03-01

    Full Text Available Tujuan penelitian ini adalah untuk menguji pengaruh asimetri informasi terhadap underpricing. Penelitian ini menggunakan sampel 63 perusahaan yang melakukan penawaran umum perdana di Bursa Efek Indonesia dalam kurun waktu 2005-2010. Analisis data menggunakan regresi linier berganda, yaitu menguji proksi asimetri informasi yang terdiri atas ukuran perusahaan, umur perusahaan, proporsi saham yang ditawarkan kepada masyarakat, reputasi underwriter dan reputasi auditor terhadap underpricing. Hasil penelitian ini mengindikasikan bahwa reputasi underwriter dan reputasi auditor berpengaruh terhadap underpricing. Ukuran perusahaan, umur perusahaan dan proporsi saham yang ditawarkan tidak berpengaruh terhadap underpricing.The aim of this research is to examine the effect of assymetric information on underpricing. This research used a sample of 63 companies that make initial public offering on the Indonesia Stock Exchange in the period of 2005-2010. The data analysis is using multiple linear regression, which is testing the proxy of asymmetric information which consists of the firm size, the firm age, the proportion of shares offered to the public, underwriter reputation and auditor reputation on underpricing. This research indicates that underwriter reputation and auditor reputation have a significant effect on underpricing. The firm size, the firm age and the proportion of shares offered to the public have no significant effect on underpricing.

  2. Fish under exercise

    NARCIS (Netherlands)

    Palstra, A.P.; Planas, J.V.

    2011-01-01

    Improved knowledge on the swimming physiology of fish and its application to fisheries science and aquaculture (i.e., farming a fitter fish) is currently needed in the face of global environmental changes, high fishing pressures, increased aquaculture production as well as increased concern on fish

  3. Supernumerary ring chromosome 20 characterized by fluorescence in situ hybridization

    NARCIS (Netherlands)

    Van Langen, Irene M.; Otter, Mariëlle A.; Aronson, Daniël C.; Overweg-Plandsoen, W.C.G.; Hennekam, Raoul C.M.; Leschot, Nico J.; Hoovers, Jan M.N.

    1996-01-01

    We report on a boy with mild dysmorphic features and developmental delay, in whom karyotyping showed an additional minute ring chromosome in 60% of metaphases. Fluorescence in situ hybridization (FISH) with a centromere specific probe demonstrated that the ring chromosome contained the centromeric

  4. Fluorescence of muscle and connective tissue from cod and salmon

    DEFF Research Database (Denmark)

    Andersen, Charlotte Møller; Wold, J.P.

    2003-01-01

    Autofluorescence of salmon and cod muscle was measured and compared with autofluorescence of collagen type I and type V. Similarities between fluorescence of fish muscle and collagen were found in that the same peaks were obtained around 390, 430, and 480 nm, These similarities are supported...

  5. Common Fluorescence In Situ Hybridization Applications in Cytology.

    Science.gov (United States)

    Savic, Spasenija; Bubendorf, Lukas

    2016-12-01

    - Fluorescence in situ hybridization (FISH) is a well-established method for detection of genomic aberrations in diagnostic, prognostic, and predictive marker testing. - To review common applications of FISH in cytology. - The published literature was reviewed. - Cytology is particularly well suited for all kinds of FISH applications, which is highlighted in respiratory tract cytology with an increasing demand for predictive FISH testing in lung cancer. Fluorescence in situ hybridization is the gold standard for detection of predictive anaplastic lymphoma kinase gene (ALK) rearrangements, and the same evaluation criteria as in histology apply to cytology. Several other gene rearrangements, including ROS proto-oncogene 1 receptor tyrosine kinase (ROS1), are becoming clinically important and share the same underlining cytogenetic mechanisms with ALK. MET amplification is one of the most common mechanisms of acquired resistance to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors and can be targeted by crizotinib. As genomic aberrations are a hallmark of malignant cells, FISH is a valuable objective ancillary diagnostic tool. In urinary tract cytology, atypical urothelial cells equivocal for malignancy are a common diagnostic dilemma and multitarget FISH can help clarify such cells. Diagnosis of malignant mesothelioma remains one of the most challenging fields in effusion cytology, and ancillary FISH is useful in establishing the diagnosis. Fluorescence in situ hybridization is a morphology-based technique, and the prerequisite for reliable FISH results is a targeted evaluation of the cells in question (eg, cancer or atypical cells). Cytopathologists and cytotechnicians should therefore be involved in molecular testing in order to select the best material and to provide their morphologic expertise.

  6. Meet the surrogate fish

    International Nuclear Information System (INIS)

    Johnson, Bob; Neitzel, Duane; Moxon, Suzanne

    1999-01-01

    This article gives details of the US Department of Energy's innovative research into the development of a sensor system that will work as a surrogate fish to provide information to aid the design of fish-friendly turbines for hydroelectric power plants. The selection of the dams for the testing of sensor fish, the release and recovery of the sensor fish, the recording of the physical forces exerted on fish as they pass through the turbines, and use of the information gathered to build more sensor fish are discussed. Fish investigations conducted at the Pacific Northwest National Laboratory are briefly described. (UK)

  7. Plastic fish

    CERN Multimedia

    Antonella Del Rosso

    2015-01-01

    In terms of weight, the plastic pollution in the world’s oceans is estimated to be around 300,000 tonnes. This plastic comes from both land-based and ocean-based sources. A lecture at CERN by chemist Wolfgang Trettnak addressed this issue and highlighted the role of art in raising people’s awareness.   Artwork by Wolfgang Trettnak. Packaging materials, consumer goods (shoes, kids’ toys, etc.), leftovers from fishing and aquaculture activities… our oceans and beaches are full of plastic litter. Most of the debris from beaches is plastic bottles. “PET bottles have high durability and stability,” explains Wolfgang Trettnak, a chemist by education and artist from Austria, who gave a lecture on this topic organised by the Staff Association at CERN on 26 May. “PET degrades very slowly and the estimated lifetime of a bottle is 450 years.” In addition to the beach litter accumulated from human use, rivers bring several ki...

  8. Three Kinds of Fish

    DEFF Research Database (Denmark)

    Høst, Jeppe Engset

    2012-01-01

    There are three kinds of fish. Fish you were given, fish you bought and fish you lease. This might sound a bit odd, but it is nevertheless the basis for the activities of Danish commercial fishers since the introduction of transferable fishing concessions (TFCs) in 2007. In the current 2012 reform...... of market based systems are wild speculation, concentration and monopolization of fishing access and subsequent leasing with fishing communities and new entrants very likely being worse off (see for example the chapter “From fishing rights to financial derivatives” is this volume or Olson 2011; Sumaila 2010...... will examine five Danish fishing operations and discuss how they have reacted in different ways to the newly introduced system of transferable fishing concessions. By introducing TFCs as a solution to fleet overcapacity, the EU Commission will also be introducing a system where buying, selling and leasing...

  9. DAMPAK NEGATIF PERKEMBANGAN TEKNOLOGI INFORMATIKA DAN KOMUNIKASI DAN CARA ANTISIFASINYA

    Directory of Open Access Journals (Sweden)

    I Gede Ratnaya

    2011-03-01

    Full Text Available Sampai saat ini perkembangan ilmu pengetahuan telah menghantarkan masyarakat menuju babak baru yaitu babak yang memanfaatkan peralatan-peralatan yang merupakan hasil dari teknologi. Penggunaan tenaga manusia yang semakin hari semakin kecil volumenya sering kali menyebabkan orang kehilangan pekerjaannya karena tugasnya telah tergantikan oleh peralatan atau mesin. Sebagai sarana penyampaian informasi dan komunikasi, komputer bisa dipakai sebagai sarana berinternetan. Lewat internet orang bisa mencari bermacam-macam informasi dan berkomunikasi. Peran yang dapat diberikan oleh aplikasi teknologi informasi ini adalah mendapatkan informasi untuk kehidupan pribadi seperti informasi tentang kesehatan, hobi, rekreasi, dan rohani. Selain memberikan keuntungan, ternyata peralatan teknologi informasi dan komunikasi juga memberikan dampak negatif bagi penggunanya. Dampak negatif tersebut muncul sebagai akibat dari penggunaan yang salah atau tidak bertanggung jawab dari yang menggunakan. Beberapa dampak negatif tersebut adalah 1. Anak lebih banyak menghabiskan waktu menonton TV ketimbang melakukan hal lainnya (seperti belajar dan olah raga, 2. Anak kehilangan kemampuan berbaur dengan masyarakat dan cenderung nyaman dengan kehidupan online, 3 Adanya pelanggaran hak cipta, 4.  Kejahatan di internet, 5. Penyebaran virus komputer, dan 6. Pornografi, perjudian, penipuan, tayangan kekerasan. Adapun cara mengatasi dampak-dampak negatif tersebut adalah : 1. Gunakan teknologi untuk menjalin hubungan dengan orang yang sudah dikenal, 2. Cari komunitas positif  yang sering melakukan pertemuan didunia nyata, 3. Perlunya penegakkan hukum yang berlaku dengan dibentuknya polisi internet, 4. Menghindari pemakaian telepon seluler yang berfitur canggih oleh anak-anak dibawah umur dan lebih mengawasi penggunaan telepon seluler, 5. Perbanyak membaca buku-buku yang bersifat edukatif dan bersifat keimanan serta aplikasi komputer yang bersifat mendidik, dan 6. Perlunya pengaturan

  10. KETERGANTUNGAN ONLINE GAME DAN PENANGANANNYA

    Directory of Open Access Journals (Sweden)

    Ridwan Syahran

    2015-06-01

    Full Text Available Penelitian ini dimaksudkan untuk mengetahui perilaku yang ditimbulkan dari kecanduan bermaian online game pada siswa SMP Negeri 1 Palu yang meliputi faktor-faktor penyebab kecanduan, keadaan psikologis yang ditimbulkan dari kecanduan bermain online game dan dampak-dampak yang ditimbulkan dari kecanduan bermain online game.Pendekatan penelitian ini adalah penelitian kualitatif dengan jenis penelitian studi kasus dengan berdasarkan responden dan informan sebagai bahan sumber data. Dalam proses pengumpulan data digunakan teknik observasi dan wawancara, dimana setelah data diperoleh selanjutnya dianalisis dengan menggunakan analisis deskriptif kualitatif.Berdasarkan hasil penelitian ini, bahwa subyek (RZ dan (MT, kedua subyek penelitian menjadi ketergantungan atau kecanduan untuk bermain online game yang dikarenakan tersedianya beberapa fasilitas pendukung untuk bermain game di rumah, adanya faktor sosial dari pertemanan dengan teman bermain dan keingintahuan yang sangat besar terhadap suatu jenis game. Penanganan ketergantungan game online dengan bantuan dari berbagai pihak terutama pihak sekolah memberikan kepercayaan kepada konselor sekolah atau guru BK untuk mengadakan seminar kepada orang tua siswa tentang game online dan masalah yang akan ditimbulkan, mengatur waktu belajar dan bermain anak, memasukkan materi tentang game online dan dampaknya kepada siswa serta memberikan penyaluran yang baik dalam memilih game yang edukatif serta Menjalin komunikasi interpersonal agar anak dapat terbuka dengan orang tua.

  11. DISTRIBUSI, KELIMPAHAN DAN VARIASI UKURAN LARVA IKAN DI ESTUARIA SUNGAI MUSI

    Directory of Open Access Journals (Sweden)

    Eko Prianto

    2016-03-01

    Full Text Available Penelitian tentang distribusi, kelimpahan dan ukuran larva ikan dilakukan pada bulan Maret, Mei, Juni dan Oktober 2011. Stasiun pengambilan contoh meliputi Muara Delta Upang (stasiun 1, Muara Sungai Musi (stasiun 2 dan Pulau Payung (stasiun 3. Pengambilan larva pada siang hari menggunakan Bongo net yang berukuran mata jaring 250 µm. Hasil identifikasi diperoleh 13 famili ditinjau menurut musim, pada bulan Mei dan Oktober masing-masing diperoleh 7 famili, dan pada bulan juni sebanyak 3 famili. Kelimpahan larva ikan berkisar antara 9-46 ind/m3 dengan jumlah yang tertinggi (46 ind/m3 pada bulan Mei dan terendah pada bulan Juni (9 ind/m3. Larva ikan dari famili Gobiidae memiliki sebaran yang cukup luas baik spasial maupun temporal. Variasi ukuran larva ikan menurut famili setiap bulannya memiliki variasi ukuran yang hampir sama.  Research about the distribution, abundance and size of fish larvae was conducted in March, May, June and October 2011. Sampling stations encompasses Delta Upang (station 1, Muara Sungai Musi (station 2 and Pulau Payung (station 3. Larvae taken during the daytime using a Bongo net with mesh size of 250 µm. Identification results obtained 13 families based on the season, in May and October respectively 7 families, and in June as many as 3 families. Abundance of fish larval around 9-46 ind/m3 with the highest number (46 ind/m3 in May and the lowest in June (9 ind/m3. Larvae of Gobiidae family have a large distribution on spatial and temporal. The variation in size of fish larvae by family on each month are the same.

  12. MEMAHAMI DAN MENGANTISIPASI DAMPAK PERUBAHAN IKLIM PADA PESISIR DAN LAUT DI INDONESIA BAGIAN TIMUR

    Directory of Open Access Journals (Sweden)

    Ibnu Sofian

    2014-06-01

    Full Text Available Indonesia mempunyai peran yang cukup besar dalam negosiasi internasional untuk mengelola dampak dari perubahan iklim. Peran tersebut antara lain dengan menyelenggarakan the 13th Conference of the Parties to the UNFCCC di Bali yang menghasilkan the Bali Action Plan. Peran pada isu perubahan iklim di atas merupakan konsekuensi logis dari besarnya bahaya dan kerentanan wilayah Indonesia terhadap fenomena global tersebut, antara lain di wilayah pesisir dan laut. Secara lebih spesifik, wilayah Indonesia bagian timur memiliki karakteristik bahaya dan kerentanan terhadap perubahan iklim. Kenaikan muka air laut dengan laju sekitar 0,6 – 0,8 cm/tahun, yang dapat menimbulkan dampak pada wilayah pemukiman, infrastruktur, dan ekosistem di wilayah pesisir serta pulau-pulau kecil. Sementara itu, peningkatan intensitas El Niño dan La Niña menyebabkan terjadinya perubahan pola arus dan sirkulasi laut seperti Arus Lintas Indonesia di Selat Makassar yang akan berdampak pada hasil tangkapan ikan. Selanjutnya, karakteristik gelombang laut juga mengalami perubahan sebagai akibat dari perubahan variabilitas iklim yang berdampak pada sektor transportasi laut. Selain itu, peningkatan intensitas La Niña, menyebabkan kenaikan intensitas pemutihan terumbu karang (coral bleaching sebagai akibat dari kenaikan suhu permukaan laut yang drastis. Mengingat besarnya dampak dan kerentanan di wilayah Indonesia bagian timur tersebut, perlu disusun strategi adaptasi, yang meliputi strategi proteksi, akomodasi, dan retreat-adaptation.   Indonesia has provided substantial role in international negotiations to manage the impacts of climate change. Roles include the holding of the 13th Conference of the Parties to the UNFCCC in Bali who produce the Bali Action Plan. Role in climate change issues is a logical consequence of the hazard and vulnerability of Indonesia to the global phenomenon, including in coastal areas and sea. More specifically, the eastern part of Indonesia has the

  13. The covert world of fish biofluorescence: a phylogenetically widespread and phenotypically variable phenomenon.

    Directory of Open Access Journals (Sweden)

    John S Sparks

    Full Text Available The discovery of fluorescent proteins has revolutionized experimental biology. Whereas the majority of fluorescent proteins have been identified from cnidarians, recently several fluorescent proteins have been isolated across the animal tree of life. Here we show that biofluorescence is not only phylogenetically widespread, but is also phenotypically variable across both cartilaginous and bony fishes, highlighting its evolutionary history and the possibility for discovery of numerous novel fluorescent proteins. Fish biofluorescence is especially common and morphologically variable in cryptically patterned coral-reef lineages. We identified 16 orders, 50 families, 105 genera, and more than 180 species of biofluorescent fishes. We have also reconstructed our current understanding of the phylogenetic distribution of biofluorescence for ray-finned fishes. The presence of yellow long-pass intraocular filters in many biofluorescent fish lineages and the substantive color vision capabilities of coral-reef fishes suggest that they are capable of detecting fluoresced light. We present species-specific emission patterns among closely related species, indicating that biofluorescence potentially functions in intraspecific communication and evidence that fluorescence can be used for camouflage. This research provides insight into the distribution, evolution, and phenotypic variability of biofluorescence in marine lineages and examines the role this variation may play.

  14. Incremental benefits from the increasing in the production of koi fish Cyprinus carpio var. koi culture

    Directory of Open Access Journals (Sweden)

    Iis Diatin

    2017-07-01

    Full Text Available ABSTRACT  Koi fish is one of the species included in the intensification program of ornamental fish production. Production of koi has only reached 82.04% of total national production target thus making it potential for development. The objective of the study was to assess additional financial benefit of production increment through stocking pattern modification. Present research was performed using case study method on Pokdakan PBC Fish Farm (PPFF, koi fish farmers in Sukabumi. Financial analysis consisted of business analysis, investment criteria, and sensitivity. Stocking pattern management could increase ornamental fish production and its benefit margin up to 1.5 times higher. That investment criteria has shown NPV at value of IDR3,824 million, net BCR 4.96, IRR 86.0%, and PP 1.7 years. Koi fish farming was sensitive to a decline in survival rate and insensitive to the rise of formulated feed price. Keywords: business analysis, koi, investment criteria, production pattern, sensitivity  ABSTRAK  Ikan koi merupakan salah satu ikan yang termasuk dalam program penguatan produksi ikan hias Indonesia. Capaian dari target produksi ikan koi yang ditetapkan pemerintah hanya mencapai 82,04 %, sehingga budidaya ikan koi potensial untuk dikembangkan dan ditingkatkan produksinya. Metode yang digunakan adalah studi kasus pada kelompok pembudidaya ikan hias koi PPFF di Sukabumi. Analisis finansial yang digunakan meliputi analisis usaha, kriteria investasi dan sensitivitas. Pengaturan pola tebar dapat meningkatkan jumlah produksi ikan hias dan marjin keuntungan hingga 1,5 kali. Analisis kriteria investasi menghasilkan nilai NPV sebesar Rp3.824 juta, net B/C 4,94, IRR 86,0% dan PP 1,7 tahun. Budidaya ikan koi sensitif terhadap penurunan kelangsungan hidup dan tidak sensitif terhadap peningkatan harga pakan buatan. Kata kunci: analisis usaha, koi, kriteria investasi, pola tebar, produksi

  15. [Performance evaluation of a fluorescamine-HPLC method for determination of histamine in fish and fish products].

    Science.gov (United States)

    Kikuchi, Hiroyuki; Tsutsumi, Tomoaki; Matsuda, Rieko

    2012-01-01

    A method for the quantification of histamine in fish and fish products using tandem solid-phase extraction and fluorescence derivatization with fluorescamine was previously developed. In this study, we improved this analytical method to develop an official test method for quantification of histamine in fish and fish products, and performed a single laboratory study to validate it. Recovery tests of histamine from fillet (Thunnus obesus), and two fish products (fish sauce and salted and dried whole big-eye sardine) that were spiked at the level of 25 and 50 µg/g for T. obesus, and 50 and 100 µg/g for the two fish products, were carried out. The recoveries of histamine from the three samples tested were 88.8-99.6% with good repeatability (1.3-2.1%) and reproducibility (2.1-4.7%). Therefore, this method is acceptable for the quantification of histamine in fish and fish products. Moreover, surveillance of histamine content in food on the market was conducted using this method, and high levels of histamine were detected in some fish products.

  16. Atomic-fluorescence spectrophotometry

    International Nuclear Information System (INIS)

    Bakhturova, N.F.; Yudelevich, I.G.

    1975-01-01

    Atomic-fluorescence spectrophotometry, a comparatively new method for the analysis of trace quantities, has developed rapidly in the past ten years. Theoretical and experimental studies by many workers have shown that atomic-fluorescence spectrophotometry (AFS) is capable of achieving a better limit than atomic absorption for a large number of elements. The present review examines briefly the principles of atomic-fluorescence spectrophotometry and the types of fluorescent transition. The excitation sources, flame and nonflame atomizers, used in AFS are described. The limits of detection achieved up to the present, using flame and nonflame methods of atomization are given

  17. Fluorescence of irradiated hydrocarbons

    International Nuclear Information System (INIS)

    Gulis, I.G.; Evdokimenko, V.M.; Lapkovskij, M.P.; Petrov, P.T.; Gulis, I.M.; Markevich, S.V.

    1977-01-01

    A visible fluorescence has been found out in γ-irradiated aqueous of carbohydrates. Two bands have been distinguished in fluorescence spectra of the irradiated solution of dextran: a short-wave band lambdasub(max)=140 nm (where lambda is a wave length) at lambdasub(β)=380 nm and a long-wave band with lambdasub(max)=540 nm at lambdasub(β)=430 nm. A similar form of the spectrum has been obtained for irradiated solutions of starch, amylopectin, lowmolecular glucose. It has been concluded that a macromolecule of polysaccharides includes fluorescent centres. A relation between fluorescence and α-oxiketon groups formed under irradiation has been pointed out

  18. Karakteristik Habitat dan Keberadaan Larva Aedes spp. pada Wilayah Kasus Demam Berdarah Dengue Tertinggi dan Terendah di Kota Tasikmalaya

    Directory of Open Access Journals (Sweden)

    Muhammad Umar Riandi

    2017-06-01

    Full Text Available Abstract. Dengue hemorrhagic fever is a vector‐borne disease caused by dengue virus and transmitted by Aedes spp. controlling the mosquito population is currently the only means to prevent dengue outbreaks. Thehighest dengue case in Tasikmalaya City until July 2015 was in Cikalang village dan the lowest in Cibunigeulis village. This research was an observational study with a cross‐sectional design with the aim to determine vector density and Aedes sp. presence risk factor based on habitat characteristic in the highest and lowest dengue cases regions. Aedes spp. breeding sites and their characteristic were examined indoor and outdoor at 100 house each in Cikalang dan Cibunigeulis village. The result showed that in Cikalang, larval presence was mostly found in a non‐water‐reservoir containers (18.4%, indoor (6.5%, made of cement/soil/rubber (11.1%, open lid (7.5%, <1 litre volume (14.6%, without draining (22.2%, without larvivorous fish (6.5%, with temephos poured (20.3%, and water company household water source (7,7%. As in Cibunigeulis larval habitats was mostly found in a non‐water‐reservoir containers (8.7%, indoor (0.9%, made of plastic/ceramic/metal/glass (0.9%, open lid (1.1%, <1 litre volume (4%, without draining (2.2%, without larvivorous fish (0.9%, without temephos poured (0.9%, and non‐water company household water source (0.9%. Binary logistic regression analysis showed that household water source (p=0,021, OR=13,78 and drainage (p=0,001, OR=0,101 as a risk factor in Aedes larvae inhabit at Cikalang village and none for Cibunigeulis village. These results showed the importance effect of containers draining factor to the presence of Aedes spp. larvae in the highest dengue cases region.Keywords: Aedes spp., larvae habitat, density, habitat characteristic, TasikmalayaAbstrak. Demam Berdarah Dengue merupakan penyakit menular yang disebabkan oleh virus dengue dan disebarkan oleh nyamuk Aedes spp. Pengendalian populasi nyamuk

  19. POTENSI MAGGOT UNTUK PENINGKATAN PERTUMBUHAN DAN STATUS KESEHATAN IKAN

    Directory of Open Access Journals (Sweden)

    Melta Rini Fahmi

    2016-11-01

    Full Text Available Penggunaan maggot sebagai pakan alternatif ikan telah dikaji di Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot merupakan larva serangga black soldier (Hermetia illusence yang dapat mengkonversi material organik menjadi biomassanya. Salah satu keunggulan maggot adalah dapat diproduksi dalam berbagai ukuran, sesuai dengan kebutuhan. Penyimpanan maggot pada suhu rendah dapat menghambat pertumbuhan dan mempertahankan kehidupannya. Produksi maggot pada ukuran kecil dimulai dari penyediaan telur, penetasan, dan pembesaran dalam media PKM (Palm Kernel Meal atau bungkil kelapa sawit, pemanenan dan penyimpanan dalam suhu rendah. Nilai nutrisi maggot pada umur 6-7 hari adalah protein: 60,2%; lemak: 13,3%; abu: 7,7%; karbohidrat: 18,8%. Percobaan pemanfaatan maggot sebagai suplemen pakan diujikan terhadap ikan Balashark (Balantiocheilus melanopterus Bleeker ukuran 2,0 ± 0,2 g. Hasil yang didapatkan menunjukkan bahwa pemberian maggot memberikan pertumbuhan dan sintasan yang lebih baik. Dampak penambahan maggot pada ikan terlihat signifikan terhadap gambaran darah ikan yang menunjukkan daya tahan tubuh ikan yang lebih baik. Maggot utilization as fish feed alternative has been studied at Loka Riset Budidaya Ikan Hias Air Tawar (LRBIHAT, Depok. Maggot is an insect larvae of black soldier (Hermetia illusence that can convert organic material to its body biomass. One of the advantages in maggot culture is that it can be produced in different sizes according to fish requirement. Keeping maggot at low temperature can delay its growth while keeping it alive. Production of small size maggot starts from eggs preparation, hatching, and rearing in media of PKM (Palm Kernel Meal or coconut oil cake of palm, cropping and then keeping it in low temperature. Nutritional value of maggot at the age of 6-7 days is as follows: protein, 60.2%, fat; 13.3%, ash; 7.7%, carbohydrate; 18.8%. Trial feeding using maggot as feed supplement was done on Balashark

  20. Evaluation of the damage in fish spermatozoa cryopreservation

    Science.gov (United States)

    Li, Jun; Liu, Qinghua; Zhang, Shicui

    2006-12-01

    Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.

  1. Marker chromosome 21 identified by microdissection and FISH

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Y.; Palmer, C.G. [Indiana Univ. School of Medicine, Indianapolis, IN (United States); Rubinstein, J. [Univ. Affiliated Cincinnati Center for Developmental Disorders, OH (United States)] [and others

    1995-03-27

    A child without Down`s syndrome but with developmental delay, short stature, and autistic behavior was found to be mosaic 46,XX/47,XX,+mar(21) de novo. The marker was a small ring or dot-like chromosome. Microdissection of the marker was performed. The dissected fragments were biotinylated with sequence-independent PCR as a probe pool for fluorescence in situ hybridization (FISH). FISH results suggested an acrocentric origin of the marker. Subsequent FISH with {alpha}-satellite DNA probes for acrocentric chromosomes and chromosome-specific 21 and 22 painting probes confirmed its origin from chromosome 21. 14 refs., 3 figs.

  2. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.

    Directory of Open Access Journals (Sweden)

    David F Gruber

    Full Text Available We report the identification and characterization of two new members of a family of bilirubin-inducible fluorescent proteins (FPs from marine chlopsid eels and demonstrate a key region of the sequence that serves as an evolutionary switch from non-fluorescent to fluorescent fatty acid-binding proteins (FABPs. Using transcriptomic analysis of two species of brightly fluorescent Kaupichthys eels (Kaupichthys hyoproroides and Kaupichthys n. sp., two new FPs were identified, cloned and characterized (Chlopsid FP I and Chlopsid FP II. We then performed phylogenetic analysis on 210 FABPs, spanning 16 vertebrate orders, and including 163 vertebrate taxa. We show that the fluorescent FPs diverged as a protein family and are the sister group to brain FABPs. Our results indicate that the evolution of this family involved at least three gene duplication events. We show that fluorescent FABPs possess a unique, conserved tripeptide Gly-Pro-Pro sequence motif, which is not found in non-fluorescent fatty acid binding proteins. This motif arose from a duplication event of the FABP brain isoforms and was under strong purifying selection, leading to the classification of this new FP family. Residues adjacent to the motif are under strong positive selection, suggesting a further refinement of the eel protein's fluorescent properties. We present a phylogenetic reconstruction of this emerging FP family and describe additional fluorescent FABP members from groups of distantly related eels. The elucidation of this class of fish FPs with diverse properties provides new templates for the development of protein-based fluorescent tools. The evolutionary adaptation from fatty acid-binding proteins to fluorescent fatty acid-binding proteins raises intrigue as to the functional role of bright green fluorescence in this cryptic genus of reclusive eels that inhabit a blue, nearly monochromatic, marine environment.

  3. UJI TOKSISITAS DAN PERUBAHAN STRUKTUR MIKROANATOMI INSANG IKAN NILA LARASATI (Oreochromis nilloticus YANG DIPAPAR TIMBAL ASETAT

    Directory of Open Access Journals (Sweden)

    FAM Mulyani

    2014-11-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui Nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati yang dipapar timbal; mengetahui perubahan struktur  mikroanatomi dan mengetahui pada konsentrasi berapa timbal menyebabkan perubahan struktur mikroanatomi insang. Sampel uji toksisitas sebanyak 120 ekor untuk mengetahui tingkat  kematian ikan hingga 50% dalam 96 jam, dan uji perlakuan sebanyak 80 ekor dengan konsentrasi 0 ppm; 259,51 ppm; 291,94 ppm; dan 324,38 ppm. Pada akhir minggu ke empat diambil sampel insang dan dilakukan analisis gambaran struktur mikroanatomi insang secara diskriptif. Hasil analisis menunjukkan kerusakan edema 0%-25%, fusi lamela antara 1%-75%, hiperplasia 0%-50%, epithelial lifting 0%-50%, dan nekrosis 0%-50%. Dari hasil penelitian dapat disimpulkan bahwa nilai  LC50-96 jam pada uji toksisitas ikan Nila Larasati sebesar 324,38 ppm dan mulai terjadi perubahan struktur mikroanatomi insang pada konsentrasi 259,51 ppm. The objective of thje study was to determine the value of LC50-96 hours in a toxicity test of Nila Larasati fish exposed to lead acetate; to determine the changes in the microanatomy structure and to determine the concentration of lead acetate which causes microanatomy structure changes in the gills of Nila Larasati fish. The samples were 120 fish to find out the 50% fish mortality within 96 hours, and 80 fish in treatment test with the lead acetate concentrations of 0 ppm; 259.51 ppm; 291.94 ppm; and 324.38 ppm. At the end of the 4th week of the research, the gill samples were taken and the analysis of the microanatomy structure was carried out descriptively. The result of the analysis showed that there were 0%-25% edema, 1%-75% lamella fusion, 0%-50% hyperplasia, 0%-50% epithelial lifting and 0%-50% necrosis. It was concluded that the value of LC50-96 hours in Nila Larasati fish toxicity test was 324.38 ppm; and microanatomy changes started to occur in the lead acetate concentration of 259.51 ppm. 

  4. PENGARUH FLUKTUASI TINGGI MUKA AIR TERHADAP HASIL TANGKAPAN IKAN DI SUNGAI DAN RAWA MAHAKAM HULU KALIMANTAN TIMUR

    Directory of Open Access Journals (Sweden)

    Kamaluddin Kasim

    2015-12-01

    Full Text Available Beberapa penelitian menyebutkan bahwa fluktuasi tinggi muka air (TMA dapat mempengaruhi hasil tangkapan ikan di perairan sungai dan rawa namun tidak terhadap semua jenis ikan.  Penelitian ini dilakukan dengan tujuan untuk mengetahui hasil tangkapan jenis ikan sungai dan rawa sungai Mahakam yang mendapat pengaruh fluktuasi TMA dan jenis ikan yang tidak mendapatkan pengaruh langsung oleh fluktuasi TMA. Data mengenai hasil tangkapan ikan yang berasal dari alat tangkap pancing dan jaring diperoleh melalui enumerator di Tempat Pendaratan Ikan (TPI Selili Kota Samarinda pada periode 2007-2012, sedangkan nilai rata-rata Tinggi Muka Air (TMA DAS Mahakam secara bulanan diperlukan sebagai salah satu faktor yang diduga berpengaruh terhadap hasil tangkapan beberapa jenis ikan sungai dan rawa. Data dianalisis dengan metode regresi linear sederhana dan penentuan perbedaan hasil tangkapan pada musim hujan, peralihan dan kemarau dilakukan dengan Analysis of Variance (ANOVA. Hasil penelitian menunjukkan bahwa jumlah hasil tangkapan ikan berkorelasi kuat (r=0,7 terhadap Tinggi Muka air (TMA dengan arah hubungan negatif atau berkebalikan, yakni semakin tinggi nilai TMA maka hasil tangkapan semakin rendah. Jenis ikan sungai dan rawa seperti patin, nila, sepat siam (Trichogaster pectoralis, lais dan betok (Anabas testudineus merupakan jenis ikan yang hasil tangkapannya dipengaruhi secara signifikan (P0,05 oleh fluktuasi TMA.   Some studies have showed that water level fluctuation may have a significant correlation to the catch of several commercial fish target in inland fishery and does not influence directly the cath of some commercial fish. This study aimed to determine which species are directly influenced and such species not inluenced by water level fluctuation for its catches. Catch data obtained from hand line and gill net are recorded by enumerators at Fish Landing Sites of Selili, Samarinda during the period of 2007-2012, while the data of surface water

  5. STRATEGI PEGELOLAAN SUAKA PERIKANAN RAWA BANJIRAN DI SUMATERA DAN KALIMANTAN

    Directory of Open Access Journals (Sweden)

    Agus Djoko Utomo

    2016-10-01

    “Danau lindung Empangau “ (124 ha di Kapuas Hulu Kalimantan Barat terdapat stok ikan 5.700 ekor/ha didominansi oleh ikan Haruan (Channa striata, Entukan (Thynnichthys thynnoides, Biawan (Helostoma temmenckii, Tengadak (Barbonymus schwanenfeldii. Suaka perikanan tipe sungai “Kapak Hulu” (segmen sungai panjang 1 km, lebar 30 m di Lubuk Lamam Sumatera selatan terdapatstok ikan 4 ton didominansi oleh ikan Lais (Kryptopterus kryptopterus, Baung (Hemibagrus nemurus, Palau (Osteochilus hasselti, Lampam (Barbonymus schwanenfeldii. Suaka perikanan “Lubuk Gunung Isam” di Lubuk Lamam Sumatera selatan terdapat stok ikan 2 ton didominansi oleh ikan Tapa (Wallago leeri, Bulu Tulang (Kryptopterus apagon, Baung (Hemibagrus nemurus, Lais (Kryptopterus kryptopterus. Agar supaya suaka perikanan dapat berfungsi dengan baik sehingga mempunyai dampak terhadap masyarakat sekitarnya maka suaka tersebut harus dikelola dengan tepat mulai dari penentuan lokasi yang tepat, sarana prasarana yang diperlukan, serta kelembagaan dan pengawasan. Floodplain waters are strongly influenced by season, drought during dry season and floods in rainy season. There are two groups of fish in the swamp namely the swamp fishgroup (black fish and river fish group (white fish. Based on the type of habitat in the swamp flood, there are several types of fish reserves namely floodplain pool reserve, swamp lake reserve, river segment reserve, and deep pool of the riverreserve. Floodplain pool reserve type:” Suak Buaya Reserve”(0.5 ha in southern Sumatra contained 2 tons of fish stocks dominated by snakehead, kissing gourami, Snakeskin gourami, climbing perches. Swamp lake reserve type: Empangau Lake Reserve (124 ha in West Kalimantan with5.700 fish/ha dominated by Snakehead fish, minnows or carp,kissing gourami, Tinfoil barb. River segment reserve type: “Kapak Hulu Reserve “ (river segment, length =1 km and width =30 m in southern Sumatra contained 4 tons of fish dominated by Sheatfishes, Catfish

  6. GAMBARAN JENIS DAN JUMLAH KONSUMSI FAST FOOD DAN SOFT DRINK PADA MAHASISWA OBESITAS DI UNIVERSITAS HASANUDDIN

    OpenAIRE

    Suryanti, Rut; Jafar, Nurhaedar; Syam, Aminuddin

    2013-01-01

    Konsumsi fast food dan soft drink dapat menyebabkan kelebihan berat badan dan obesitas.Penelitian ini bertujuan untuk mengetahui gambaran jenis dan jumlah zat gizi makro dari konsumsi fast food dan soft drink pada mahasiswa yang obesitas.Jenis penelitian adalah deskriptif, dilaksanakan pada bulan Mei-Juni 2013 di Universitas Hasanuddin dengan jumlah responden 60 orang. Instrument penelitian adalah kuesioner identitas diri dan food frekuensiSemiQuantitatif fast food dan soft drink, food pictur...

  7. PERSEPSI DAN TRANSFORMASI VISI DAN MISI PADA CIVITAS AKADEMIKA STAIN PEKALONGAN

    Directory of Open Access Journals (Sweden)

    Imam Khanafi

    2013-05-01

    Full Text Available Penelitian ini mengukur tingkat persepsi visi dan misi civitas akademika STAIN Pekalongan, dan mengukur juga tingkat implementasi sebagai transformasi visi misi tersebut serta menemukan hubungan antara persepsi dengan implementasinya. Kajian ini memiliki signifikan yang strategis dalam konteks manajemen dan pengembangan Pendidikan di STAIN Pekalongan dan lembaga pendidikan lainnya. Hal ini karena posisi visi dan misi sebagai kerangka acuan (frame of reference yang mengarahkan dan menjelaskan kepada keseluruh unsur civitas akademika dalam setiap program kerjanya.

  8. Membranes and Fluorescence microscopy

    DEFF Research Database (Denmark)

    Bagatolli, Luis

    2009-01-01

    Fluorescence spectroscopy-based techniques using conventional fluorimeters have been extensively applied since the late 1960s to study different aspects of membrane-related phenomena, i.e., mainly relating to lipid-lipid and lipid-protein (peptide) interactions. Even though fluorescence...

  9. Multimodal fluorescence imaging spectroscopy

    NARCIS (Netherlands)

    Stopel, Martijn H W; Blum, Christian; Subramaniam, Vinod; Engelborghs, Yves; Visser, Anthonie J.W.G.

    2014-01-01

    Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample.

  10. SEJARAH PENGUASAAN SUMBER DAYA PESISIR DAN LAUT DI TELUK TOMINI

    Directory of Open Access Journals (Sweden)

    Muhammad Obie

    2015-01-01

    Full Text Available This article aims to analyze the historical milestones of coastal and sea resources management in Tomini Bay. It used a critical theory paradigm with two strategies, namely historical sociology and case studies. The collected data were primary and secondary ones, then were analyzed by using qualitative approach. The analysis results indicated that coastal and sea management in To-mini Bay could be divided into era before 1901, when Bajo Tribe was the sea adventurer in To-mini Bay as well as owning the resources. Since 1901 to independence era of Old Order, Bajo tribe began to settle to coastal area, built houses above the sea surfaces with economic resources coming from fishing and other sea pickings.  During the New Order, precisely from 1977 to Reformation Order, the existence of Bajo Tribe was terribly disturbed by the wood company, fishpond, and conservation policy. In this era, Bajo Tribe faced the resettlement pressure that caused their community was divided, Sea Bajo and Land Bajo.  This reality caused the access of the Land Bajo community to the coastal and sea resources was limited, while the Sea Bajo community was progressively under the pressure of of the expansion of the wood company, fishpond, and conservation policy.Key words: Bajo Tribe, wood company, fishpond, conservation, resettlement, cultural tourismTujuan penelitian ini adalah menganalisis tonggak-tonggak sejarah penguasaan sumber daya pesisir dan laut di Teluk Tomini. Penelitian ini menggunakan paradigma teori kritis, dengan strategi sosiologi sejarah dan studi kasus. Data yang terkumpul berupa data primer dan data sekunder, kemudian dianalisis dengan pendekatan kualitatif. Hasil penelitian menunjukkan bahwa penguasaan sumber daya pesisir dan laut di Teluk Tomini dapat dibagi atas masa sebelum tahun 1901, yang ditandai Suku Bajo sebagai pengembara laut di Teluk Tomini sekaligus me-nguasai sumber daya yang ada. Sejak tahun 1901 sampai masa kemerdekaan (Orde Lama, Suku Bajo

  11. Hawaiian Fish Distributors Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This is proprietary sales data from one Kona-based fish dealer for August 1986 to Decemeber 1988. Fishing was generally around Kona. This is Dealer Data and is...

  12. Pittsburgh Fish Fry Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Lenten Fish Fry records for the Greater Pittsburgh region. Data is collected before and during the Lenten fish fry season each year by Code for Pittsburgh. Data is...

  13. Fishing fleet profiling methodology

    National Research Council Canada - National Science Library

    Ferraris, Jocelyne

    2002-01-01

    A fishing fleet profile aims tho assist in understanding the complexity and structure of fisheries from a technical and socio-economic point of view, or from the point of view of fishing strategies...

  14. Poisoning - fish and shellfish

    Science.gov (United States)

    Fish poisoning; Dinoflagellate poisoning; Seafood contamination; Paralytic shellfish poisoning; Ciguatera poisoning ... algae and algae-like organisms called dinoflagellates. Small fish that eat the algae become contaminated. If larger ...

  15. Scorpion fish sting

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/002849.htm Scorpion fish sting To use the sharing features on this page, please enable JavaScript. Scorpion fish are members of the family Scorpaenidae, which includes ...

  16. Fish population dynamics

    National Research Council Canada - National Science Library

    Gulland, J. A

    1977-01-01

    This book describes how the dynamics of fish populations can be analysed in terms of the factors affecting their rates of growth, mortality and reproduction, with particular emphasis on the effects of fishing...

  17. Perancangan dan Analisis Redistribution Routing Protocol OSPF dan EIGRP

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2016-02-01

    Full Text Available Abstrak OSPF (Open Shortest Path First dan EIGRP (Enhanced Interior Gateway Routing Protocol adalah dua routing protokol yang banyak digunakan dalam jaringan komputer. Perbedaan karakteristik antar routing protokol menimbulkan masalah dalam pengiriman paket data. Teknik redistribution adalah solusi untuk melakukan komunikasi antar routing protokol. Dengan menggunakan software Cisco Packet Tracer 5.3 pada penelitian ini dibuat simulasi OSPF dan EIGRP yang dihubungkan oleh teknik redistribution, kemudian dibandingkan kualitasnya dengan single routing protokol EIGRP dan OSPF. Parameter pengujian dalam penelitian ini adalah nilai time delay dan trace route. Nilai trace route berdasarkan perhitungan langsung cost dan metric dibandingkan dengan hasil simulasi. Hasilnya dapat dilakukan proses redistribution OSPF dan EIGRP. Nilai delay redistribution lebih baik 1% dibanding OSPF dan 2-3% di bawah EIGRP tergantung kepadatan traffic. Dalam perhitungan trace route redistribution dilakukan 2 perhitungan, yaitu cost untuk area OSPF dan metric pada area EIGRP. Pengambilan jalur utama dan alternatif pengiriman paket berdasarkan nilai cost dan metric yang terkecil, hal ini terbukti berdasarkan perhitungan dan simulasi. Kata kunci: OSPF, EIGRP, Redistribution, Delay, Cost, Metric. Abstract OSPF (Open Shortest Path First and EIGRP (Enhanced Interior Gateway Routing Protocol are two routing protocols are widely used in computer networks. Differences between the characteristics of routing protocols pose a problem in the delivery of data packets. Redistribution technique is the solution for communication between routing protocols. By using the software Cisco Packet Tracer 5.3 in this study were made simulating OSPF and EIGRP redistribution linked by technique, then compared its quality with a single EIGRP and OSPF routing protocols. Testing parameters in this study is the value of the time delay and trace route. Value trace route based on direct calculation of cost

  18. Immunity to Fish Rhabdoviruses

    OpenAIRE

    Purcell, Maureen K.; Laing, Kerry J.; Winton, James R.

    2012-01-01

    Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabd...

  19. Sugar Cane Genome Numbers Assumption by Ribosomal DNA FISH Techniques

    NARCIS (Netherlands)

    Thumjamras, S.; Jong, de H.; Iamtham, S.; Prammanee, S.

    2013-01-01

    Conventional cytological method is limited for polyploidy plant genome study, especially sugar cane chromosomes that show unstable numbers of each cultivar. Molecular cytogenetic as fluorescent in situ hybridization (FISH) techniques were used in this study. A basic chromosome number of sugar cane

  20. Clinical consequences of using PNA-FISH in Staphylococcal bacteraemia

    DEFF Research Database (Denmark)

    Laub, R R; Knudsen, Inge Jenny Dahl

    2014-01-01

    To optimize patient treatment and rational use of antimicrobials, it is important to provide fast information on findings in blood-cultures (BCs). The purpose of this study was to evaluate the impact of using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) on positive BCs conta...

  1. Combined fluorescence in situ hybridization and microautoradiography (FISH-MAR)

    International Nuclear Information System (INIS)

    Ginige, M.P.

    2005-01-01

    Cultivation independent studies have revealed, that due to the complexity of natural ecosystems such as activated sludge, rhizosphere, rumen etc., the pure cultivation of all related micro-organisms in these diverse ecosystems is quite unsuccessful. Accordingly, the small subunit ribosomal RNA (SSU rRNA, i.e. 16S and 18S rRNA) or genes, obtained from these ecosystems without cultivation has become a widely accepted approach to describe the phylogenetic diversity of microbial communities present in these ecosystems. The rapid growth of the rRNA gene (rDNA) sequence data bank, accessible via the Internet (http://www.ncbi.nlm.nih.gov/BLAST/) has enabled us to compare microbial diversities across the globe without cultivation. However, these rRNA gene sequences provide very few direct clues regarding the interactions and the metabolic capabilities of the identified micro-organisms. Accordingly, the knowledge available on the in situ physiology of the inhabitants of most microbial ecosystems is quite remote and the availability of such knowledge will enable our ability to manipulate ecosystems (e.g. activated sludge) to achieve better process performances with the aid of improved mathematical models. In both natural and engineered systems, there are a diverse group of microorganisms cohabiting within matrix-encloses such as bio-films or flocs rather than single planktonic cells. In order to manipulate these systems to obtain higher productivity, it is important to understand population structure, dynamics, functionality and also special distributions in the matrix-encloses. It is an accepted fact that pure cultivation of micro-organisms in these complex ecosystems does not permit to resolve any of the above mentioned issues since (1) rigorous biomass disaggregation which is a common practice in cultivation-based approaches results in a loss of special information; (2) cultivation approaches also introduces significant biases resulting in pronounced population shifts resulting in an inaccurate evaluation of the natural population dynamics; and (3) the influence caused by artificial environments where cultivation studies are performed also influences gene expression of related organisms. Hence, an accurate functional analysis of the active communities in these complex communities is not feasible through pure cultivation studies

  2. Fish eye optics

    Science.gov (United States)

    Hudec, R.; Michalova, S.

    2017-07-01

    We report on small student (high—school) project of the Czech Academy of Sciences dealing with animal (fish) eyes and possible application in science and technology. Albeit most fishes have refractive eyes, the recent discoveries confirm that some fishes have reflective eyes with strange arrangements as well.

  3. Komposisi Asam Lemak, Angka Peroksida, dan Angka TBA Fillet Ikan Kakap (Lutjanus sp pada Suhu dan Lama Penyimpanan Berbeda

    Directory of Open Access Journals (Sweden)

    Rahim Husain

    2018-01-01

    Full Text Available Fish has a high nutritional value and is a major food source in many countries. Fish lipid has a high content of polyunsaturated fatty acids (PUFAs, especially eicosapentaenoic acid (EPA; 20: 5n-3 and docosahexsanoic acid (DHA; 22: 6n-3. The objective of this research was to determine fatty acids composition of snapper (Lutjanus sp fillet and its damage during the storage process. The results showed that total of the saturated fatty acids (SFA increased from 4.35% to 25.55%, 28.06%, 32.73%, and 61.75% during storage at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C, respectively. Total mono-unsaturated fatty acids (MUFA were 23.72%, 23.69%, 14.4%, 22.66%, and 29.4% at storage temperature of 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Total PUFA decreased from 25.06% to 15.98%, 14.99%, 10.32%, and 8.84% at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Peroxide value, as primary peroxide of snapper fillet, increased about 10.60 times with an increased in storage temperature from 0 °C to 40 °C. Value of TBA increased 6.60 times with an increased in temperature from 0 °C to 40 °C during 45 days. ABSTRAK Ikan memiliki nilai gizi tinggi dan merupakan sumber makanan utama di banyak negara. Lipid ikan memiliki kandungan tinggi asam tak jenuh ganda (Poly Unsaturated Fatty Acid, PUFA, terutama asam eikosapentanoat (EPA; 20:5n-3 dan asam docosahexsanoat (DHA; 22:6n-3. Penelitian ini bertujuan untuk mengetahui komposisi asam lemak fillet ikan kakap (Lutjanus sp dan kerusakan akibat proses penyimpanan. Hasil analisis asam lemak jenuh (Saturated Fatty Acid, SFA menunjukkan bahwa asam lemak jenuh meningkat dari 4,35% menjadi 25,55%, 28,06%, 32,73%, dan 61,75% selama penyimpanan pada 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Total asam lemak tak jenuh (Mono Unsaturated Fatty Acid, MUFA adalah 23,72%, 23,69, 14,4%, 22,66%, dan 29,4% pada penyimpanan 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Sedangkan total PUFA turun dari 25,05% menjadi 15,98%, 14,99%, 10,32%, dan

  4. Learning the scientific method using GloFish.

    Science.gov (United States)

    Vick, Brianna M; Pollak, Adrianna; Welsh, Cynthia; Liang, Jennifer O

    2012-12-01

    Here we describe projects that used GloFish, brightly colored, fluorescent, transgenic zebrafish, in experiments that enabled students to carry out all steps in the scientific method. In the first project, students in an undergraduate genetics laboratory course successfully tested hypotheses about the relationships between GloFish phenotypes and genotypes using PCR, fluorescence microscopy, and test crosses. In the second and third projects, students doing independent research carried out hypothesis-driven experiments that also developed new GloFish projects for future genetics laboratory students. Brianna Vick, an undergraduate student, identified causes of the different shades of color found in orange GloFish. Adrianna Pollak, as part of a high school science fair project, characterized the fluorescence emission patterns of all of the commercially available colors of GloFish (red, orange, yellow, green, blue, and purple). The genetics laboratory students carrying out the first project found that learning new techniques and applying their knowledge of genetics were valuable. However, assessments of their learning suggest that this project was not challenging to many of the students. Thus, the independent projects will be valuable as bases to widen the scope and range of difficulty of experiments available to future genetics laboratory students.

  5. KEMAMPUAN GENTENG PLASTIK BERGELOMBANG (CORRUGATED PLASTIC SEBAGAI BIOFILTER PARTIKEL AMONIAK DAN BAHAN ORGANIK DI MEDIA BUDIDAYA DAN LIMBAH CAIR BUDIDAYA IKAN (Performance of Corrugated Plastic as Biofilter of Ammonia Particle and Organic Material

    Directory of Open Access Journals (Sweden)

    Muslim Muslim

    2010-07-01

    Full Text Available ABSTRAK Pertumbuhan budidaya ikan dalam beberapa dekade ini berkembang sangat pesat, hal ini karena permintaan akan ikan meningkat. Meningkatnya kegiatan budidaya ikan selalu diiringi dengan meningkatnya limbah yang dihasilkan. Hal ini akan sangat cepat berpengaruh bila sistem budidaya yang dipakai adalah semi intesif atau intensif. Limbah tersebut harus segera dihilangkan atau dikurangi, karena akan berdampak pada ikan yang dibudidaya dan lingkungan seperti sungai dan laut. Tujuan penelitian ini adalah ingin mengetahui kemampuan genteng plastik bergelombang mengurangi limbah yang dihasilkan budidaya ikan yaitu Total Suspended Sediment (TSS, Suspended Sediment (SS, amoniak dan bahan organik (COD. Dari hasil penelitian diperoleh bahwa air limbah budidaya ikan yang mengandung TSS, SS, amoniak dan bahan organik setelah dilewatkan dengan genteng plastik bergelombang konsentrasinya menurun dengan tingkat efisiensi pengurangan yang terjadi di dalam kolam ikan dan di luar kolam ikan adalah sebagai berikut: 74,51% dan 54,42% (TSS; 39,20% dan 49,12% (SS; 19,82% dan 14,2% (amoniak; dan 24,82% dan 22,47% (COD. Ternyata genteng plastik bergelombang mempunyai tingkat pengurangan (g/m3/hr dan tingkat pengurangan spesifik (mg/m2/hr terhadap kandungan amoniak lebih efektif bila dibandingkan dengan material lain seperti plastic rolls, scrub pads, pipa PVC dan lain sebagainya.   ABSTRACT Aquaculture has been developing rapidly during the last few decades; it is due to the increase of fish demand. Increasing aquaculture activities especially with semi-intensive and intensive system have significant effect on waste production, which has to be removed or to be reduced quickly because will effect on fish in rearing tank and environment when through away to environment such as river and sea. The objectives of this study were to know the capability of corrugated plastic to remove or to reduce wastes content produced by aquaculture activities, i.e, Total Suspended

  6. Pengaruh Perebusan, Penggaraman Dan Penjemuran Pada Udang Dan Cumi Terhadap Pembentukan 7 Ketokolesterol

    Directory of Open Access Journals (Sweden)

    Rudi Riyanto

    2007-12-01

    Full Text Available Penelitian pengaruh perebusan, penggaraman dan penjemuran pada udang dan cumi terhadap pembentukan 7‑ketokolesterol telah dilakukan. Dalam penelitian ini, udang dan cumi masing ­masing dibagi dalam dua kelompok. Pada kelompok pertama diberikan perlakuan perebusan dalam air, dan pada kelompok kedua diberikan perlakuan perebusan dalam air garam (3% air garam untuk udang dan 20% air garam untuk cumi. Setelah perebusan, dilakukan pengeringan di bawah matahari (udang dikupas dahulu sebelum dijemur. Pengambilan sampel dilakukan saat kondisi segar, setelah direbus dan setelah dijemur. Pengamatan dilakukan terhadap kandungan 7‑ketokolesterol, kolesterol, nilai TBA, dan kadar air. Hasil penelitian menunjukkan bahwa perebusan, penggaraman dan penjemuran berpengaruh terhadap pembentukan 7‑ketokolesterol sampai konsentrasi 20,82 ppm pada udang dan 15,05 ppm pada cumi sebagai konsentrasi tertinggi produk hasil oksidasi kolesterol yang terbentuk. Penelitian ini dapat dijadikan bukti bahwa pemanasan (perebusan dan penjemuran, serta penggaraman berpengaruh dalam proses oksidasi kolesterol pada udang dan cumi.

  7. Fluorescence and Spectral Imaging

    Directory of Open Access Journals (Sweden)

    Ralph S. DaCosta

    2007-01-01

    Full Text Available Early identification of dysplasia remains a critical goal for diagnostic endoscopy since early discovery directly improves patient survival because it allows endoscopic or surgical intervention with disease localized without lymph node involvement. Clinical studies have successfully used tissue autofluorescence with conventional white light endoscopy and biopsy for detecting adenomatous colonic polyps, differentiating benign hyperplastic from adenomas with acceptable sensitivity and specificity. In Barrett's esophagus, the detection of dysplasia remains problematic because of background inflammation, whereas in the squamous esophagus, autofluorescence imaging appears to be more dependable. Point fluorescence spectroscopy, although playing a crucial role in the pioneering mechanistic development of fluorescence endoscopic imaging, does not seem to have a current function in endoscopy because of its nontargeted sampling and suboptimal sensitivity and specificity. Other point spectroscopic modalities, such as Raman spectroscopy and elastic light scattering, continue to be evaluated in clinical studies, but still suffer the significant disadvantages of being random and nonimaging. A recent addition to the fluorescence endoscopic imaging arsenal is the use of confocal fluorescence endomicroscopy, which provides real-time optical biopsy for the first time. To improve detection of dysplasia in the gastrointestinal tract, a new and exciting development has been the use of exogenous fluorescence contrast probes that specifically target a variety of disease-related cellular biomarkers using conventional fluorescent dyes and novel potent fluorescent nanocrystals (i.e., quantum dots. This is an area of great promise, but still in its infancy, and preclinical studies are currently under way.

  8. CORPORATE IDENTITY SEJARAH DAN APLIKASINYA

    Directory of Open Access Journals (Sweden)

    Christine Suharto Cenadi

    1999-01-01

    Full Text Available In the recent globalization era%2C and the developed market%2C many companies compete to attract consumers to buy their products. One of the keys to compete and survive in this developing market is by creating an image and graphic identity. This paper will discuss about corporate identity%2C image%2C its purpose and applications. Abstract in Bahasa Indonesia : Dalam era globalisasi dewasa ini%2C dan dengan berkembangnya pasar (market%2C banyak perusahaan bersaing untuk menarik perhatian konsumen untuk membeli produknya. Salah satu cara untuk bersaing dan dapat bertahan di dalam pasar yang terus berkembang ini adalah dengan menciptakan suatu image dan identitas graphic. Tulisan ini akan membahas tentang corporate identity%2C image%2C fungsi dan aplikasinya

  9. Profil Sensori dan Nilai Gizi Beberapa Jenis Ikan Patin dan Hibrid Nasutus

    Directory of Open Access Journals (Sweden)

    Theresia Dwi Suryaningrum

    2010-11-01

    Full Text Available Penelitian mengenai profil sensori dan nilai gizi filet patin Siam (Pangasius hypopthalmus, Jambal (Pangasius djambal Bleeker, Pasupati, Nasutus serta hasil silangan Siam dan Nasutus (hibrid Nasutus telah dilakukan. Analisis sensori dilakukan dengan uji pembeda menyeluruh, uji pembeda atribut, uji kesukaan, dan uji rangking. Pengamatan lainnya dilakukan terhadap edible portion dan nilai gizi (proksimat dan profil asam amino. Hasil penelitian menunjukkan bahwa filet patin hibrid Nasutus lebih memiliki kesamaan warna dengan filet patin Nasutus daripada filet patin Siam. W arna daging filet patin hibrid Nasutus berbeda nyata dengan induknya yaitu patin Nasutus dan patin Siam. Panelis lebih menyukai warna filet patin hibrid Nasutus dibandingkan dengan patin Pasupati. Patin hibrid Nasutus mempunyai tekstur yang berbeda nyata dengan Nasutus dan Jambal yang kompak dan padat, tetapi mempunyai kesamaan dengan patin Siam dan Pasupati yang agak kompak dan agak padat. Berdasarkan intensitas warna, hasil uji pembeda atribut dan uji kesukaan, maka secara berturut-turut panelis menyukai filet patin Jambal, Nasutus, hibrid Nasutus, Pasupati, dan Siam. Hibrid Nasutus mempunyai edible portionpaling tinggi (49% dibandingkan dengan patin lainnya tetapi mempunyai kadar air, kadar lemak, dan kadar protein yang lebih rendah dan berbeda nyata dengan induknya (patin Siam dan Nasutus. Patin Siam mengandung asam amino esensial paling tinggi di antara berbagai jenis patin yang diteliti. Profil asam amino patin hibrid Nasutus, Jambal, Pasupati, dan Nasutus hampir sama, kecuali pada patin Siam yang mengandung glisin, leusin, isoleusin, histidin, serin, treonin, dan prolin yang lebih tinggi dibandingkan dengan patin lainnya

  10. POLA USAHA DAN PENDAPATAN RUMAH TANGGA PETANI PADA BERBAGAI TIPOLOGI LAHAN RAWA LEBAK

    Directory of Open Access Journals (Sweden)

    Nasir Nasir

    2015-11-01

    Full Text Available The study aim to 1 Describing of business pattern developed farmer households in various typologies of lowland swamp land, and 2 Analyzing household income of farmers in various businesses developed in lowland swamp land. The sampling method used is a simple random sample with a total of  responden  as many as 222 farmers. The study used a survey method. The results showed that the business pattern of households at shallow swampy, ie: Horticulture: annual fruit, Livestock: chickens and goats, trade, household industry, transport services, Rice, fishing, horticulture: vegetables and fruits a season and crops: cassava and peanuts, Labour, fish farming. The business pattern of households at middle swamp, ie: horticulture: annual fruit, Livestock: ducks, trade, household industry, transport services, rice, fishing, horticulture: vegetables and fruits a season, Labor, fish farming. The business pattern of households at deep swampy, ie: Horticulture: annual fruit, Livestock: duck and buffalo, trade, household industry, transport services, rice, fishing, horticulture: vegetables and fruits a season, Labor, fishing, fish farming. Household income in each of the  swampy land, ie: household income  the shallow swampy amounted Rp19.525.400/year.  The household income  the middle swampy amounted Rp20.212.000/year.  The household income the deep swampy amounted Rp18.248.000/yearKeywords: pattern of business, income, household, lebak, surveyABSTRAKTujuan penelitian ini 1 mendeskripsikan pola usaha yang dikembangkan rumah tangga petani pada berbagai tipologi lahan rawa lebak, dan 2 menghitung pendapatan rumah tangga petani pada berbagai pola usaha yang dikembangkan pada lahan rawa lebak. Metode penarikan contoh yang digunakan adalah sampel acak sederhana (random sampling dengan jumlah total sebanyak 222 orang petani. Metode penelitian yang digunakan adalah metode survei. Hasil penelitian menunjukan bahwa pola usaha yang dikembangkan petani lahan rawa

  11. MENGENALI GEJALA TINITUS DAN PENATALAKSANAANNYA

    Directory of Open Access Journals (Sweden)

    Dewi Purwita Agustini

    2016-05-01

    Full Text Available Telinga berdenging atau dikenal dalam bahasa medis sebagai tinitus, banyak dikeluhkan sebagai suatu bising atau bunyi yang muncul di kepala tanpa adanya rangsangan dari luar. Adapun keluhan yang dialami ini seperti bunyi mendengung, mendesis, menderu, atau berbagai variasi bunyi yang lain. Tinitus bukanlah penyakit atau sindroma, tapi hanya merupakan gejala yang mungkin berasal dari satu atau sejumlah kelainan. Sebetulnya suara yang terdengar oleh telinga tersebut belum tentu bersifat kelainan atau patologis. Jika orang sehat yang terbukti telinganya normal, berada dalam ruang kedap (anehoic chamber, maka ia akan dapat mendengar berbagai macam suara yang berasal dari berbagai organ tubuhnya sendiri yang memang bekerja setiap saat, contohnya: pernapasan, kontraksi jantung, dan aliran darah. Kenyataannya, dalam kehidupan sehari-hari, suasana yang memungkinkan suara fisiologis atau normal tersebut terdengar oleh seseorang sangat jarang tercipta dan bahkan dalam kamar yang sunyi di malam hari sekalipun. Hal ini dikarenakan, bunyi masking dari lingkungan dengan berintensitas bunyi sekitar 25 – 30 dB. Tinitus baru menjadi gejala jika suara organ tubuh intensitasnya melebihi bunyi masking lingkungan tadi. Tinitus kerap diderita terutama orang pada kelompok usia pertengahan dan usia tua. Menurut data statistic dari pusat kesehatan di Amerika, sekitar 32% orang dewasa pernah mengalami tinitus pada suatu saat tertentu dalam hidupnya, dan 6 % nya sangat menganggu dan cukup sulit disembuhkan. Di Inggris, 17% populasi juga memiliki masalah tinitus. Sayangnya di Indonesia belum ada data statistic yang memadai, namun berdasarkan pengalaman empiris, penderita tinitus cukup banyak dan sering ditemui di tempat praktek, klinik, maupun rumah sakit. Meski tinitus bukanlah keadaan yang membahayakan, munculnya gejala ini pada hampir kebanyakan orang sangat mengganggu dan sering mempengaruhi kualitas hidup dan pekerjaannya. Kata Kunci: Tinitus, berdenging, telinga

  12. Le CRDI dans les Philippines

    International Development Research Centre (IDRC) Digital Library (Canada)

    Le CRDI appuie la recherche dans les. Philippines depuis 1972. Le travail qu'y ont accompli des universités et des instituts de recherche vigoureux et la participation active de la société civile ont entraîné d'importantes améliorations dans les domaines de l'agriculture, du suivi de la pauvreté et de la gestion des forêts.

  13. XML specifications DanRIS

    DEFF Research Database (Denmark)

    2009-01-01

    XML specifications for DanRIS (Danish Registration- og InformationsSystem), where the the aim is: Improved exchange of data Improved data processing Ensuring future access to all gathered data from the year 1999 until now......XML specifications for DanRIS (Danish Registration- og InformationsSystem), where the the aim is: Improved exchange of data Improved data processing Ensuring future access to all gathered data from the year 1999 until now...

  14. Ketergantungan Online Game Dan Penanganannya

    OpenAIRE

    Syahran, Ridwan

    2015-01-01

    Penelitian ini dimaksudkan untuk mengetahui perilaku yang ditimbulkan dari kecanduan bermaian online game pada siswa SMP Negeri 1 Palu yang meliputi faktor-faktor penyebab kecanduan, keadaan psikologis yang ditimbulkan dari kecanduan bermain online game dan dampak-dampak yang ditimbulkan dari kecanduan bermain online game.Pendekatan penelitian ini adalah penelitian kualitatif dengan jenis penelitian studi kasus dengan berdasarkan responden dan informan sebagai bahan sumber data. Dalam proses ...

  15. Fluorescent discharge lamp

    Science.gov (United States)

    Mukai, E.; Otsuka, H.; Nomi, K.; Honmo, I.

    1982-01-01

    A rapidly illuminating fluorescent lamp 1,200 mm long and 32.5 mm in diameter with an interior conducting strip which is compatible with conventional fixtures and ballasts is described. The fluorescent lamp is composed of a linear glass tube, electrodes sealed at both ends, mercury and raregas sealed in the glass tube, a fluorescent substance clad on the inner walls of the glass tube, and a clad conducting strip extending the entire length of the glass tube in the axial direction on the inner surface of the tube.

  16. Highly thermostable fluorescent proteins

    Science.gov (United States)

    Bradbury, Andrew M [Santa Fe, NM; Waldo, Geoffrey S [Santa Fe, NM; Kiss, Csaba [Los Alamos, NM

    2011-03-22

    Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

  17. IMPLEMENTASI TQM BERORIENTASI HARD SKILL DAN SOFT SKILL DALAM PEMBELAJARAN SEJARAH SMA DI KOTA SEMARANG

    Directory of Open Access Journals (Sweden)

    Cahyo Budi Utomo

    2011-10-01

    Full Text Available This study aims to introduce the implementation of hardskill and softskill oriented TQM in teacher’s learning of history in Senior High School in Semarang. The research model are structural fit model and significance of relationship between TQM implementation model, PDSA cycle, the model analysis of fish bone, continuous improvement , hard skills and soft skills and qualified history learning is the main target of research. Data was obtained from field studies using Likert scale questionnaire developed from the constructs and theoretical construct indicator. Analysis performed using software models SmartPLS. Implementation of PDSA cycle correlated 0879 to the implementation of fish bone analysis, correlates 0830 PDSA cycle to continuously improvement , while continuously improvement  correlated 0441 and 0749 against the hard skills to soft skills. Soft skills to hard skills correlate of 0329, while the correlation of hard skills to qualified history learning is at 0673. The developed qualityfied history learning need support from  the realization of the continuous (continuously improvement and optimal orientation of hard skills. Keywords: model, TQM, PDSA, the analysis of fish bone, hard skills, soft skills, learning the history of quality  Kajian ini bertujuan mengenalkan model implementasi TQM yang berorientasi pada hardskill dan softskill dalam pembelajaran sejarah di sekolah atas Semarang pada para guru. Sebagai model penelitian, model struktural fit dan signifikasi  dari korelasi antara model implementasi TQM, siklus PDSA, model analisis tulang ikan, perbaikan terus-menerus, hard skill dan soft skill, dan pembelajaran sejarah bermutu (PSB menjadi target utama penelitian. Data didapatkan dari studi lapangan menggunakan angket skala Likert yang dikembangkan dari konstruk dan indicator konstruk teoretis. Analisis menggunakan program SmartPLS. Implementasi siklus PDSA berkorelasi 0879 terhadap implementasi model analisis

  18. Turbine related fish mortality

    International Nuclear Information System (INIS)

    Eicher, G.J.

    1993-01-01

    A literature review was conducted to assess the factors affecting turbine-related fish mortality. The mechanics of fish passage through a turbine is outlined, and various turbine related stresses are described, including pressure and shear effects, hydraulic head, turbine efficiency, and tailwater level. The methodologies used in determining the effects of fish passage are evaluated. The necessity of adequate controls in each test is noted. It is concluded that mortality is the result of several factors such as hardiness of study fish, fish size, concentrations of dissolved gases, and amounts of cavitation. Comparisons between Francis and Kaplan turbines indicate little difference in percent mortality. 27 refs., 5 figs

  19. ENERGI DAN DAMPAKNYA TERHADAP LINGKUNGAN

    Directory of Open Access Journals (Sweden)

    I Made Astra

    2010-11-01

    Full Text Available Energi dapat berubah dari satu bentuk ke bentuk yang  lainnya, perubahannya sering mempengaruhi lingkungan dan udara yang kita hirup dengan berbagai cara. Energy kimia dalam bahan bakar fosil diubah menjadi energy panas, mekanik, atau listrik melalui pembakaran dan  ini sebagai penghasil polutan terbesar. Dan dengan demikian pembangkit listrik, kendaraan bermotor, dan kompor adalah penyebab utama terjadinya polusi udara.  Polutan yang dikeluarkan biasanya dikelompokan menjadi  hidrokarbon (HC, nitrogen oksida (NOx, dan  karbon monoksida (CO. Polutan yang dihasilkan pada pembakaran fosil merupakan faktor terbesar terjadinya asap, hujan asam,  pemanasan global dan perubahan iklim.   The conversion of energy from one form to another often affects the environment and the air we breath in many ways. Pollutants are emitted as the chemical energy in fossil fuels is converten to thermal, mechanical, or electrical energy via combustion, and thus power plants, motor vehicles, and even stoves take the blame for air pollution, and  the pollutants released by the vehicles are usually grouped as hydrocarbons (HC, nitrogen oxides (NOx, and carbon monoxide (CO. Pollutans emitted during the combustion of fossil fuels are responsible for smog, acid rain, and global warming and climate change.

  20. Isolasi dan Karakterisasi Ralstonia syzygii

    Directory of Open Access Journals (Sweden)

    Yanuar Danaatmadja

    2009-07-01

    Full Text Available Ralstonia syzygii is one of important pathogens of cloves. This study was aimed at obtaining pure culture of R. syzygi from diseased plant tissue samples. The acterium was isolated from infected clove plantations in Ungaran, Central Java. It had the ability to make clove seedlings show symptoms and then die 41 days after inoculation. The reisolated bacterium also showed its ability to kill clove seedlings after 17 days. The bacterium is gram negative, able to ultilize glucose, sucrose, maltose, mannitol, sorbitol, dulcitol, and glycerol, able to hydrolyze starch, grow well at 27oC, able to catalyze and release water and oxygen from hydrogen peroxyde (H2O2 and aerobically.   Ralstonia syzygii merupakan salah satu patogen penting pada tanaman cengkeh. Penelitian ini dilakukan untuk mendapakan isolat murni R syzygii dari jaringan tanaman terinfeksi. Bakteri diisolasi dari pertanaman cengkeh sakit yang berasal dari daerah Ungaran, Jawa Tengah. Bakteri menunjukkan kemampuan untuk menyebabkan bibit tanaman cangkeh sakit dan kemudian mati 41 hsi. Bakteri hasil reisolasi juga menunjukkan kemampuannya menyebabkan sakit dan kematian pada bibit tanaman cengkeh setelah 17 hsi. Bakteri yang diperoleh bersifat gram negatif, mampu memanfaatkan glukosa, sukrosa, maltosa, manitol, sorbitol, dulsitol, dan gliserol, mampu menghidrolisis pati, tumbuh baik pada suhu 27o C, mampu mengkatalisis dan melepaskan air dan oksigen dari hydrogen peroxide (H2O2 dan bersifat aerob.

  1. INTERAKSI VARIABILITAS IKLIM DENGAN EKOSISTEM TERUMBU KARANG DAN SUMBER DAYA IKAN KARANG

    Directory of Open Access Journals (Sweden)

    Reny Puspasari

    2015-12-01

    Full Text Available Keberadaan ikan karang di perairan dapat dipengaruhi oleh beberapa faktor, antara lain: kondisi ekosistem terumbu karang, lingkungan perairan di sekitarnya dan perubahan iklim. Variabilitas iklim ditandai oleh besar kecilnya nilai dari Southern Oscillation Index (SOI, Dipole Mode Index (DMI dan curah hujan. Hubungan antara variabilitas iklim dengan ekosistem terumbu karang dan sumber daya ikan karang di wilayah perairan Sumatera Barat dianalisis dengan menggunakan analisis deskriptif terhadap data temporal, analisis komponen utama dan uji-t pada nilai CPUE (catch per unit effort  ikan karang dari tahun-tahun terjadinya La Nina dan El Nino. Hasil penelitian menunjukkan bahwa SOI mempengaruhi kondisi ekosistem terumbu karang dan nilai CPUE ikan karang. Pemutihan karang di perairan Sumatera Barat pada 1997 terjadi ketika peristiwa El Nino Ekstrim negatif yang berasosiasi dengan IODM (Indian Ocean Dipole Mode positif kuat. Nilai CPUE ikan karang lebih tinggi pada saat terjadi La Nina bila dibandingkan dengan nilai saat peristiwa El Nino. Rata-rata CPUE ikan karang pada saat terjadi peristiwa La Nina adalah 67 kg/trip, sementara pada saat terjadi peristiwa El Nino rata-rata nilai CPUE ikan karang adalah 32 kg/trip. Faktor DMI mempengaruhi peningkatan konsentrasi klorofil-a perairan sebagai tanda terjadi peningkatan kesuburan perairan. Status of reef fishes are highly influenced by several factors, such as: coral reef condition, adjacent waters condition and climate change. Climate variability is indicated by the values of Southern Oscillation Index (SOI, Dipole Mode Index (DMI and rain fall. Interaction between climate variability and coral reef ecosystem and reef fish condition were analyzed by using a descriptive analysis to the temporal data, principal component analysis and t-test for CPUE of reef fishes in a certain years when La Nina and El Nino occurred. The results show that SOI influenced the coral reef ecosystem and reef fishes CPUE. Coral

  2. Fish allergy: in review.

    Science.gov (United States)

    Sharp, Michael F; Lopata, Andreas L

    2014-06-01

    Globally, the rising consumption of fish and its derivatives, due to its nutritional value and divergence of international cuisines, has led to an increase in reports of adverse reactions to fish. Reactions to fish are not only mediated by the immune system causing allergies, but are often caused by various toxins and parasites including ciguatera and Anisakis. Allergic reactions to fish can be serious and life threatening and children usually do not outgrow this type of food allergy. The route of exposure is not only restricted to ingestion but include manual handling and inhalation of cooking vapors in the domestic and occupational environment. Prevalence rates of self-reported fish allergy range from 0.2 to 2.29 % in the general population, but can reach up to 8 % among fish processing workers. Fish allergy seems to vary with geographical eating habits, type of fish processing, and fish species exposure. The major fish allergen characterized is parvalbumin in addition to several less well-known allergens. This contemporary review discusses interesting and new findings in the area of fish allergy including demographics, novel allergens identified, immunological mechanisms of sensitization, and innovative approaches in diagnosing and managing this life-long disease.

  3. Do Fish Resist?

    Directory of Open Access Journals (Sweden)

    Dinesh Joseph Wadiwel

    2016-03-01

    Full Text Available There have been a number of scientific studies on the question of whether fish feel pain. Some have suggested that some fish indeed do feel pain and that this has significant welfare implications (2003. Others have argued that fish do not have the brain development necessary to feel pain. In terms of number of animals killed, the slaughter of sea animals for human consumption significantly exceeds that of any land animals that we use for food, and sea animal slaughter practices frequently lack any basic welfare protections. If fish can be shown to feel pain—or more importantly, if humans can agree that fish feel pain—then this would place a significant question mark over many contemporary fishing practices.  This article substitutes the question 'Do Fish Feel Pain?' with an alternative: 'Do Fish Resist?' It explores the conceptual problems of understanding fish resistance, and the politics of epistemology that surrounds and seeks to develop a conceptual framework for understanding fish resistance to human capture by exploring the development of fishing technologies - the hook, the net and contemporary aquaculture.

  4. Augmented fish health monitoring

    International Nuclear Information System (INIS)

    Michak, P.; Rogers, R.; Amos, K.

    1991-05-01

    The Bonneville Power Administration (BPA) initiated the Augmented Fish Health Monitoring project in 1986. This project was a five year interagency project involving fish rearing agencies in the Columbia Basin. Historically, all agencies involved with fish health in the Columbia Basin were conducting various levels of fish health monitoring, pathogen screening and collection. The goals of this project were; to identify, develop and implement a standardized level of fish health methodologies, develop a common data collection and reporting format in the area of artificial production, evaluate and monitor water quality, improve communications between agencies and provide annual evaluation of fish health information for production of healthier smolts. This completion report will contain a project evaluation, review of the goals of the project, evaluation of the specific fish health analyses, an overview of highlights of the project and concluding remarks. 8 refs., 1 fig., 4 tabs

  5. GAYA HIDUP, KONSUMSI PANGAN, DAN HUBUNGANNYA DENGAN TEKANAN DARAH PADA LANSIA ANGGOTA POSBINDU

    Directory of Open Access Journals (Sweden)

    Ruth Ayu Wulandari

    2016-01-01

    Full Text Available ABSTRACTThe purpose of this study was to determine the relationship of life style and food consumption to blood pressure among elderly in Posbindu Al-Wusto in Bogor District. The study design was cross-sectional study. Subjects were consisted of eight men and 31 women from Posbindu Al-Wusto in Bogor District. The result showed most subject had hypertension (64.1%. There was no significant relationship between life style (smoking, coffee consumption, and exercise with blood pressure (p>0.05. There was significantly negative relationship between hypertension preventive food (broccoli and sunflower seeds and hypertension triggering food (crackers and salted fish with systolic blood pressure (p<0.05. There was significantly positive food high blood pressure induce (boiled fish with systolic blood pressure (p<0.05.Keywords: blood pressure, elderly, food consumption, life styleABSTRAKPenelitian ini bertujuan untuk mengetahui hubungan gaya hidup dan konsumsi pangan dengan tekanan darah pada lansia di Posbindu Al-Wusto Kabupaten Bogor. Desain penelitian ini adalah cross-sectional. Subjek terdiri atas delapan orang laki-laki dan 31 orang perempuan dari Posbindu Al-Wusto Kabupaten Bogor. Hasil penelitian menunjukkan bahwa sebagian besar subjek (64,1% mempunyai hipertensi. Tidak terdapat hubungan yang signifikan antara gaya hidup (merokok, konsumsi kopi, dan olahraga dengan tekanan darah (p>0,05. Terdapat hubungan signifikan negatif antara pangan pencegah (brokoli dan biji bunga matahari dan pangan pemicu (crackers dan ikan asin dengan tekanan darah sistolik (p<0,05. Terdapat hubungan signifikan positif antara pangan pemicu (ikan pindang dengan tekanan darah sistolik (p<0,05.Kata kunci: gaya hidup, konsumsi pangan, lansia, tekanan darah

  6. Pengaruh Iodium terhadap Perubahan Fungsi Tiroid dan Status Iodium

    Directory of Open Access Journals (Sweden)

    Suryati Kumorowulan

    2015-01-01

    Full Text Available ABSTRACT Iodine is an essential element that enables the thyroid gland to produce thyroid hormones. The source of iodine in the body comes from food, iodized salt and iodine capsules. Iodized salt and iodine capsules had been used by the government in Iodine Deficiency Disorders (IDD elimination program. However, iodine capsules prophylaxis program in the IDD endemic areas had caused symptoms of hyperthyroidism, subsequently the program was discontinued since 2009. The aim of this research to determine the effect of iodine supplementation on thyroid function and iodine status. This was quasi-experimental design, conducted in Pituruh Purworejo District. Sample of this study consisted of 85 women of childbearing age, divided into three intervention groups. Each group obtained iodized salt for one month, then continued with the provision of iodized salt and 200 mg iodine capsules in group I, iodized salt and 400 mg iodine capsules in group II, and iodized salt and shredded tuna fish 2 times a week in group III for 3 months. Thyroid status was measured by levels of TSH, FT4 analyzed with ELISA. Iodine status measured by UIE levels analyzed with spectrophotometer. Data was analyzed using General Linear Model Repeated Measure (GLM RM. After 3 months, in all three treatment groups the TSH changes were statistically significant. In FT4 hormone levels, after administration of the intervention occurred “Wolff-Chaikoff” mechanism. There were significant increase on Iodine status (UIE (p< 0.05 in all three treatment groups. There is a change in iodine status and thyroid function after administration of three forms of iodine intervention. Keywords: iodine, TSH, FT4, UIE. ABSTRAK Iodium merupakan bahan dasar untuk membentuk hormon tiroid. Sumber iodium tubuh berasal dari makanan, garam beriodium, dan kapsul iodium. Garam beriodium dan kapsul iodium telah digunakan oleh pemerintah dalam penanggulangan Gangguan Akibat Kekurangan Iodium (GAKI. Namun peng

  7. KOMPOSISI UKURAN, NISBAH KELAMIN DAN DAERAH PENYEBARAN HIU BUAYA (Pseudocarcharias kamoharai YANG TERTANGKAP DI SAMUDERA HINDIA

    Directory of Open Access Journals (Sweden)

    Dian Novianto

    2016-05-01

    Full Text Available Ikan hiu buaya (Pseudocarcharias kamoharai merupakan salah satu jenis hiu yang tertangkap secara tidak sengaja pada perikanan rawai tuna (tuna longline. Ikan hiu buaya dalam penanganannya selalu dibuang di laut karena ukuran tubuhnya yang kecil dan tidak memiliki pasar diantara ikan non target (by-catch lainnya. Penelitian tentang komposisi ukuran, nisbah kelamin dan daerah penyebaran hiu buaya dilakukan pada tahun 2010 – 2011 di perairan WPP 573 – 572 Samudera Hindia dengan mengikuti kapal longline yang berbasis di Benoa dan Bungus. Tujuan penelitian adalah untuk mendapatkan data dan informasi dalam rangka pengelolaan dan konservasi hiu buaya. Hiu buaya memiliki kisaran panjang tubuh 37 – 96 cm FL dengan panjang maksimal 96 cm FL untuk jenis betina dan 94 cm FL untuk jenis jantan dan modus pada kisaran 85 – 89 cm FL untuk jenis betina dan 75 – 79 cm FL untuk jenis jantan. Perbandingan jenis kelamin ikan jantan dan betina adalah 1 : 1,33, menunjukkan bahwa rasio ikan jantan dan betina tidak seimbang. Ikan hiu buaya terdistribusi tidak merata, hanya terdapat di 77 posisi setting pada 1120 – 1220 Bujur Timur dan 090 – 150 Lintang Selatan dari 256 jumlah setting selama periode penelitian ini.   Crocodile shark (Pseudocarcharias kamoharai is one of sharks species caught accidentally in tuna longline fisheries. Crocodile shark handling is always discarded at sea because of it is small size and is not economics value among others non-target fish (by-catch. Research on the biology of crocodile shark was conducted in 2010 – 2011 at Fisheries management Areas (FMA 572 (Indian Ocean – west Sumatera and 573 (South of Java – East Nusa Tenggara following longline vessels based in Benoa and Bungus. The purpose of research was to investigate data and information on the crocodile shark for management and conservation purposes. Crocodile shark has a range of body length of 37-96 cm FL with a maximum length of 96 cm FL for females and 94 cm

  8. KEBIJAKAN PENGOPERASIAN BUBU DENGAN ALAT BANTU TERUMBU KARANG BUATAN DAN RUMPON DI WILAYAH REHABILITASI PERAIRAN KEPULAUAN SERIBU

    Directory of Open Access Journals (Sweden)

    Isa Nagib Edrus

    2014-05-01

    Full Text Available Ujicoba bubu bersama terumbu karang buatan dan rumpon di wilayah rehabilitasi perairan Pulau Pari dan Pramuka adalah untuk menentukan kesesuaian paket teknologi alat tangkap dan alat bantu dalam usaha perikanan yang layak dari sisi teknis, sosial dan ekonomi. Tulisan ini merupakan sintesa kebijakan pola pemanfaatan bubu dengan dua alat bantu pengumpul ikan. Pendekatan yang digunakan adalah analisis kebijakan dengan memformulasikan semua informasi yang relevan dan hasil penelitian terkait. Hasil analisis menunjukkan bahwa penggunaan bubu dalam perikanan multi alat tangkap dengan pemanfaatan alat bantu penangkapan tergolong efektif secara teknis, sosial dan ekonomis. CPUE bubu dari 4 hari perendaman/trip adalah ratarata 1,1 kg/unit/trip untuk nelayan Pulau Pari yang mengoperasikan bubu di dekat terumbu buatan dan 2,4 kg/unit/trip untuk nelayan Pulau Pulau Pramuka yang menaruh bubu di bawah rumpon. Perikanan bubu tergolong layak ekonomi jika terintegrasi dengan perikanan multi alat tangkap. Nilai tambah pendapatan dari penggunaan bubu berkisar pada Rp. 196.000–Rp. 400.000 per trip. Perikanan bubu memiliki prospek yang baik untuk dikembangkan dalam skala besar melalui diversifikasi usaha perikanan di wilayah perairan yang direhabilitasi dan aplikasinya dapat diterima nelayan. Alat bantu rumpon memberikan pengaruh lebih besar pada hasil tangkap bubu dibanding hanya penggunaan karang buatan.   The pot application by using artificial reefs and payaos as rehabilited fishing grounds in the waters of Pari and Pramuka Islands was to determine technological, social and economical feasible fisheries used pots and the both of fish aggregation divices (FAD. This paper is policy making of pot utility design using two kinds of the FAD. Policy analysis is an approach used to formulate any relevant information and related study results. The analysis showed that pot applications in multi gears fisheries using FAD were effective by technical, social and

  9. Reviews in fluorescence 2007

    CERN Document Server

    Lakowicz, Joseph R; Geddes, Chris D

    2009-01-01

    This fourth volume in the Springer series summarizes the year's progress in fluorescence, with authoritative analytical reviews specialized enough for professional researchers, yet also appealing to a wider audience of scientists in related fields.

  10. Introduction to fluorescence

    CERN Document Server

    Jameson, David M

    2014-01-01

    "An essential contribution to educating scientists in the principles of fluorescence. It will also be an important addition to the libraries of practitioners applying the principles of molecular fluorescence."-Ken Jacobson, Kenan Distinguished Professor of Cell Biology and Physiology, University of North Carolina at Chapel Hill"An exquisite compendium of fluorescence and its applications in biochemistry enriched by a very exciting historical perspective. This book will become a standard text for graduate students and other scientists."-Drs. Zygmunt (Karol) Gryczynski and Ignacy Gryczynski, University of North Texas Health Science Center"… truly a masterwork, combining clarity, precision, and good humor. The reader, novice or expert, will be pleased with the text and will not stop reading. It is a formidable account of the fluorescence field, which has impacted the life sciences so considerably in the last 60 years."-Jerson L. Silva, M.D., Ph.D., Professor and Director, National Institute of Science and Tech...

  11. Fluorescence (Multiwave) Confocal Microscopy.

    Science.gov (United States)

    Welzel, J; Kästle, Raphaela; Sattler, Elke C

    2016-10-01

    In addition to reflectance confocal microscopy, multiwave confocal microscopes with different laser wavelengths in combination with exogenous fluorophores allow fluorescence mode confocal microscopy in vivo and ex vivo. Fluorescence mode confocal microscopy improves the contrast between the epithelium and the surrounding soft tissue and allows the depiction of certain structures, like epithelial tumors, nerves, and glands. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Contribution of fluorescence in situ hybridization to biological dosimetry

    International Nuclear Information System (INIS)

    Sorokine-Durm, I.; Roy, L.; Durand, V.; Voisin, P.

    1995-01-01

    Fluorescence in situ hybridization with composite whole chromosome specific DNA probes for human chromosomes 2, 4 and 12 an α-satellite centromeric DNA probe labelled with biotin were used to measure symmetrical and terminal translocations (dose rate 0.5 Gy/min) and dicentrics (0.1 Gy/min) induced in vitro by 60 Co γ-irradiation (0-5 Gy). The suitability of fluorescence in situ hybridization (F.I.S.H.) technique for dicentrics detection is compared with the conventional technique. Dose-response curves for γ-rays ( 60 Co) for two dose rates are shown (dicentrics and translocations). (authors). 10 refs., 2 figs

  13. BIOMASA, PRODUKTIVITAS KAPAL PENANGKAP DAN POTENSI PRODUKSI IKAN DEMERSAL DI LAUT ARAFURA

    Directory of Open Access Journals (Sweden)

    Purwanto Purwanto

    2015-09-01

    Full Text Available Tulisan ini menyajikan hasil kajian potensi produksi dan upaya penangkapan optimal serta menjelaskan perkembangan perikanan demersal di Laut Arafura, termasuk pula estimasi dampak peningkatan upaya penangkapan terhadap produktivitas kapal dan hasil tangkapan ikan demersal. Kajian menggunakan data statistik produksi perikanan yang dikoreksi dengan menambahkan estimasi hasil tangkapan yang tidak tercatat dan data jumlah kapal penangkap dengan memperhitungkan pula estimasi jumlah kapal yang beroperasi secara ilegal. Berdasarkan hasil analisis, potensi produksi ikan demersal yang dapat dihasilkan secara lestari dari pemanfaatan stok ikan demersal di Laut Arafura adalah 539 ribu ton/tahun dengan upaya penangkapan sekitar 903 unit kapal pukat ikan 180 GT. Walaupun upaya penangkapan dari kapal yang memiliki surat izin penangkapan ikan di Laut Arafura tahun 2011 lebih rendah dibandingkan upaya penangkapan optimal untuk menghasilkan MSY, stok ikan demersal tersebut telah dimanfaatkan melebihi tingkat optimumnya akibat tingginya intensitas operasi kapal perikanan tanpa izin. Estimasi kerugian hasil tangkapan akibat kegiatan penangkapan ikan ilegal juga disajikan disini. This paper presents result of the assessment of potential production and optimal fishing effort, and briefly describes the past development of the demersal fishery in the Arafura Sea, including the estimated impact of increased fishing effort on the productivity of vessels and demersal fish catches. The assessment used corrected fisheries statistic data taking into account unreported catches and corrected fishing vessel data taking into account vessels operated illegally. Based on the analysis, the production potential of demersal fish that can be produced in a sustainable manner of demersal fish stocks in the Arafura Sea is 539 thousand tons/year to attempt the arrest of about 903 units of fishing trawlers 180 GT. Although fishing effort from vessels granted license in

  14. Penskalaan Butir Format Respons Pilihan dan Respons Bebas Berdasarkan Model Rasch dan Partial Credit

    OpenAIRE

    Eko Hariadi

    2007-01-01

    Penelitian melihat pengaruh jumlah parameter butir, kategori respons bebas (RB), pengaruh sampel terhadap akurasi estimasi parameter kemampuan untuk menghasilkan estimasi yang stabil dan pengaruh pembobotan butir RP dan butir RB terhadap kesalahan baku. Penelitian dalam dua tahap, simulasi menggunakan 30 kondisi dengan replikasi 50 dengan variabel panjang tes, jumlah kategori, dan jumlah parameter butir, dan analisis deskriptif, dilanjutkan penerapan penskalaan gabungan butir tipe res...

  15. Metode Sederhana dan Efektif untuk Penghitungan dan Visualisasi Tiga Dimensi (3D) Biofilm Vibriio Cholera

    OpenAIRE

    Kurniawan, Andi; Fadjar, Mohamad; Sukoso, Sukoso; Prihanto, Asep Awaludin

    2015-01-01

    Mikroorganisme yang mampu menghasilkan biofim menimbulkan masalah yang serius dalam bidang kesehatan dan pangan. Penelitian biofim bagi sebagian peneliti sangat identik dengan kerumitan proses penghitungan dan visualisasi penutupan permukaan substrat penempelan bakteri. Penelitian ini ditujukan untuk mengetahui efiiensi metode alternatif untuk menghitung dan memvisualisasikan biofimVibrio cholera. Pada penelitian ini beberapa faktor lingkungan seperti pH, suhu, dan kondisi kultur diujicobakan...

  16. North Slope, Alaska ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and freshwater fish species for the North Slope of Alaska. Vector...

  17. Columbia River ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, anadromous, and freshwater fish species in Columbia River. Vector polygons in this...

  18. Western Alaska ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine, estuarine, and anadromous fish species in Western Alaska. Vector polygons in this data set...

  19. Health effects of fish and fish oils

    National Research Council Canada - National Science Library

    Chandra, Ranjit Kumar

    1989-01-01

    .... Based on epidemiologic data, it has been suggested that a fish-containing diet is beneficial in the prevention and management of a variety of disorders including coronary heart disease, hypertension, and psoriasis...

  20. Southeast Alaska ESI: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains biological resource data for estuarine, benthic, and pelagic fish in Southeast Alaska. Vector polygons in this data set represent locations of...

  1. Fluorescence Image Segmentation by using Digitally Reconstructed Fluorescence Images

    OpenAIRE

    Blumer, Clemens; Vivien, Cyprien; Oertner, Thomas G; Vetter, Thomas

    2011-01-01

    In biological experiments fluorescence imaging is used to image living and stimulated neurons. But the analysis of fluorescence images is a difficult task. It is not possible to conclude the shape of an object from fluorescence images alone. Therefore, it is not feasible to get good manual segmented nor ground truth data from fluorescence images. Supervised learning approaches are not possible without training data. To overcome this issues we propose to synthesize fluorescence images and call...

  2. Fish elevator and method of elevating fish

    Energy Technology Data Exchange (ETDEWEB)

    Truebe, J.; Drooker, M.S.

    1984-02-14

    A means and method are disclosed for transporting fish from a lower body of water to a higher body of water. The means comprise a tubular lock with a gated entrance below the level of the lower body of water through which fish may enter the lock and a discharge passage above the level of the upper body of water. The fish raising means in the lock is a crowder pulled upward by a surface float as water from the upper body of water gravitationally flows into the closed lock filling it to the level of the upper body. Water is then pumped into the lock to raise the level to the discharge passage. The crowder is then caused to float upward the remaining distance through the water to the level of the discharge passage by the introduction of air into a pocket on the underside of the crowder. The fish are then automatically discharged from the lock into the discharge passage by the out of water position of the crowder. The movement of the fish into the discharge passage is aided by the continuous overflow of water still being pumped into the lock. A pipe may be connected to the discharge passage to deliver the fish to a selected location in the upper body of water. 6 figs.

  3. Fish elevator and method of elevating fish

    Science.gov (United States)

    Truebe, Jonathan; Drooker, Michael S.

    1984-01-01

    A means and method for transporting fish from a lower body of water to a higher body of water. The means comprises a tubular lock with a gated entrance below the level of the lower body of water through which fish may enter the lock and a discharge passage above the level of the upper body of water. The fish raising means in the lock is a crowder pulled upward by a surface float as water from the upper body of water gravitationally flows into the closed lock filling it to the level of the upper body. Water is then pumped into the lock to raise the level to the discharge passage. The crowder is then caused to float upward the remaining distance through the water to the level of the discharge passage by the introduction of air into a pocket on the underside of the crowder. The fish are then automatically discharged from the lock into the discharge passage by the out of water position of the crowder. The movement of the fish into the discharge passage is aided by the continuous overflow of water still being pumped into the lock. A pipe may be connected to the discharge passage to deliver the fish to a selected location in the upper body of water.

  4. Pengujian Kemampuan Daya Samak Cube Black dan Limbah Cair Gambir Terhadap Mutu Kulit Tersamak

    Directory of Open Access Journals (Sweden)

    Gustri Yeni

    2016-06-01

    Full Text Available Leather tanning industries generally use sintetic tanner such as chrome (Cr+3 which can damage environment. Therefore, it is needed to find environmentally friendly tannery material, one is by using gambier. The aim of this research was to know tannic ability of gambier on goat’s leather and tuna fish. The gambier for tannery material contained high tannin (>70% namely Cube black and liquid waste from gambier processing. Concentration of tannery material that would be used for tanned was 1 kg. Tanned leather was tested to tannery degree (DP and physical test included pull strength, elongation, and rip strength, at same treatment compared to chrome tannery. Test results leather tanned showed that, the higher the concentration of tannery material the higher the results of DP, and the physical characteristic was better. Tanned leather from goat that used Cube black gambier concentration at 4% gave DP value approximately equal to chrome tanner (38,45% and 36,60%. For tanned tuna fish gave value of DP were 39,57% and 31,35%. Tannery material of gambier gave value of pull strength, rip strength, and elongation were higher than chrome’s. The value of pull strength of goat’s leather was 730,37 kg cm-2, tuna fish was 353,33 kg cm-2 got from liquid waste tanner. The value of rip strength fof goat’s leather was 353,33 kg cm-2, tuna fish skin 29,96 kg cm-2, and elongation value from tuna fish skin 202,0% was gotten from Cube black gambier. The result of the research showed that tannery material of gambier had tannery characteristic that can replace tanner of chrome.ABSTRAKIndustri penyamakan kulit umumnya menggunakan bahan penyamak sintetis seperti krom (Cr+3 yang dapat merusak lingkungan. Untuk itu, perlu dicari bahan penyamak yang ramah lingkungan, diantaranya menggunakan gambir. Tujuan penelitian ini adalah mengetahui daya samak gambir terhadap kulit kambing dan ikan tuna. Gambir untuk  bahan penyamak mengandung kadar tanin tinggi (>70% adalah

  5. Concentrations of metals, PCB and dioxin and furans in fish and sediments of four lakes in Northern Quebec in 2001; Teneurs en metaux, en BPC et en dioxines et furanes dans les poissons et les sediments de quatre lacs du nord du Quebec en 2001

    Energy Technology Data Exchange (ETDEWEB)

    Laliberte, D.; Tremblay, G. [Quebec Ministere de l' Environnement, Quebec, PQ (Canada). Direction du Suivi de l' Etat de l' Environnement

    2002-07-01

    An environmental study was conducted on Chibougamau, aux Dores, Obatogamau, and Waconichi Lakes in northern Quebec in 2001. Those lakes are used by the Cree Indians for subsistence fishing and sport fishing by the rest of the Quebec population. Concentrations in heavy metals and polychlorinated biphenyls (PCB) were measured in sediments from Lakes Chibougamau and aux Dores. Heavy metals concentrations were also measured from two mining effluents and four waste samples originating from a mining waste location around the lakes. In addition, three fish species were sampled in those two lakes to evaluate the effect of mining activity on mercury levels in those species. The measurements were compared to those obtained from fish originating from Obatogamau and Waconichi Lakes. Finally, heavy metals, PCB, and dioxins and furans were measured in five fish species from the four lakes. The results of the analysis from the mining effluents revealed concentrations of the six metals included in directive 019 for the mining industry were lower than the authorized limits. However, a bio-essay performed in 2002 in the final effluent of the mining basin showed that the effluent from Copper Rand Mine presented toxicity and was not in agreement with the directive for this parameter. The sediments had low mercury concentrations. The highest concentration originated from the site near Henderson Mine I. None of the concentrations exceeded the limit and there are no negative effect expected on the fauna. Arsenic, cadmium, copper, nickel and zinc had high concentrations in sediments. The corresponding locations were near Copper Rand Mine, south of Principale Mine and at the foot of Principale Park. No PCB were detected in Chibougamau and aux Dores Lakes. Data does not indicate that the mining activity caused an increase in mercury levels in fish. Mercury levels in fish captured in 2001 in Chibougamau Lake were lower than those measured in 1999. 12 refs., 9 tabs., 12 figs.

  6. Purwarupa Sistem Integrasi Quadcopter dan Mobile Robot

    OpenAIRE

    Dharmawan, Andi; Lusiarta Putera, Christian Antonia

    2012-01-01

    AbstrakQuadcopter, juga dikenal sebagai quadrotor, adalah helikopter dengan empat motor yang dilengkapi dengan  empat buah propeller pada masing-masing motornya yang digunakan untuk terbang dan bermanuver. Pada quadcopter terdapat 3 variabel sudut yang menjadi element utama dalam pengendaliannya yaitu roll, pitch, dan yaw yang diperoleh dari penggabungan sensor accelerometer, gyroscope dan magnetometer dengan metode Direction Cosine Matrix. Pada sistem integrasi mobile robot dan quadcopter, d...

  7. Review Dendrimer : Definisi, Sintesis, Aplikasi Dan Prospektif

    OpenAIRE

    Rahmi, Dwinna

    2013-01-01

    Dendrimer merupakan makrostruktur monodisperse dengan banyak cabang yang homogen dan degree of branching (DB) 100%. Dua cara sintesis dendrimer yaitu convergent dan divergent dilakukan. Convergent dilakukan dengan reaksi kovalen antara dua dan lebih monomer. Divergent dimulai dengan pembentukan inti dilanjutkan dengan pembentukan cabang yang merupakan group fungsional yang aktif. Sejauh ini dendrimer sudah banyak diterapkan pada bidang farmasi yaitu drug delivery dan non farmasi pada proses i...

  8. Rancang Bangun Aplikasi Enkripsi dan Dekripsi Email Dengan Menggunakan Algoritma Advanced Encryption Standard Dan Knapsack

    Directory of Open Access Journals (Sweden)

    Timothy John Pattiasina

    2014-07-01

    Full Text Available Advanced Encryption Standard (AES dan Knapsack adalah dua algoritma enkripsi simetris dan asimetris yang paling sering digunakan. Penelitian ini menganalisa kedua algoritma AES dan algoritma Knapsack. Prototipe aplikasi enkripsi email ini dirancang dengan menggabungkan karateristik algoritma AES dan Knapsack untuk memecahkan masalah keamanan email. Algoritma AES digunakan untuk mengenkripsi dan deskripsi email berupa teks atau file, sedangkan Algoritma Knapsack di gunakan untuk mengenkripsi kunci AES. Enkripsi hybrid yang diterapkan pada aplikasi bertujuan untuk menambah keamanan informasi dalam sebuah jaringan.

  9. PENANGKAPAN BENIH BETUTU (Oxyeleotris marmorata (Bleeker DENGAN PANGILAR (Fish Trap MENGGUNAKAN UMPAN HIDUP

    Directory of Open Access Journals (Sweden)

    Iriansyah Iriansyah

    2016-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui alat yang cocok untuk menangkap bibit benih betutu dalam keadaan hidup dan sehat. Hasil penelitian menunjukkan bahwa hasil tangkapan pangilar dalam jumlah (ekor dan berat (gram terdapat perbedaan hasil tangkapan antara perlakuan A dan B sebesar 7 ekor dan 1050 gram.  Jumlah total hasil tangkapan Pangilar (Fish Trap baik yang menggunakan umpan hidup dan yang tidak menggunakan umpan hidup adalah 75 ekor  atau dengan berat 5350 gram (5,350 Kg  dalam 16 kali pengambilan data dengan menggunakan 30 buah alat tangkap This study aims to determine a suitable tool to capture betutu seed seedlings alive and healthy. The results showed that the amount of the catch pangilar (tail and weight (g there are differences in catches between treatment A and B by 7 tails and 1050 grams. The total amount of the catch Pangilar (Fish Trap whether using live bait and live bait are not using tail is 75 or weighing 5350 grams (5.350 kg to 16 times the retrieval of data by using 30 pieces of gear.

  10. Adoption Of Improved Fish Technologies Among Fish Farmers In ...

    African Journals Online (AJOL)

    A shortfall exists between fish supply and fish demand in the country despite the introduction of improved technology to fish farmers. This led to huge wage bill on the importation of fish to meet the protein need of the ever increasing population. This prompted this study with focus on adoption of improved fish technologies ...

  11. Immunostimulants in fish diets

    Science.gov (United States)

    Gannam, A.L.; Schrock, R.M.

    1999-01-01

    Various immunostimulants and their methods of application in fish culture are examined in this review. Important variables such as life stage and innate disease resistance of the fish; immunostimulant used, its structure and mode of action; and the fish's environment are discussed. Conflicting results have been published about the efficacy of immunostimulants in fish diets. Some researchers have had positive responses demonstrated as increased fish survival, others have not. Generally, immunostimulants enhance individual components of the non-specific immune response but that does not always translate into increased fish survival. In addition, immunostimulants fed at too high a dose or for too long can be immunosuppressive. [Article copies available for a fee from The Haworth Document Delivery Service: 1-800-342-9678. E-mail address: getinfo@haworthpressinc.com ].

  12. Vaccination in Fish

    DEFF Research Database (Denmark)

    Chettri, Jiwan Kumar

    vaccines have reduced the need for usage of antibiotics with more than 99 % since the 1980s. Fish can be vaccinated by three different administration routes: injection, immersion and oral vaccination. Injection vaccination (intraperitoneal injection of vaccine) is the most time consuming and labor...... intensive method, which however, provides the best protection of the fish. Immersion vaccination is used for immunization of a high number of small fish is cost-efficient and fast (30 sec immersion into vaccine). Oral vaccination (vaccine in feed) is the least efficient. As in higher vertebrates fish...... respond to vaccination by increasing the specific antibody titer and by activating the cellular responses. My talk will cover vaccination methods in fish, immune responses and some adverse effect of oil-adjuvanted vaccines in fish with reference to our work in rainbow trout, Oncorhynchus mykiss....

  13. KORELASI ANTARA ISLAM DAN EKONOMI

    Directory of Open Access Journals (Sweden)

    Akmal Zainal Abidin

    2015-03-01

    Full Text Available CORELATION BETWEEN ISLAM AND ECONOMY. This paper aims to identifies about the correlation between economic and Islam. Islam is the perfect religion that governs all things in life, including the economy. This is evident with the concept of  well-being which is described in the al-Quran and Sunnah. Basically the goal of every human life is to prosper, although humans make sense of  well- being with a different perspective. Most understand economics assume that welfare is the welfare of  earthly material. But to make sense of well-being with the term al-Falah, is meaning holistic wellbeing and balance between material and spiritual dimensions. al-Quran and Sunnah have taught that the human being will be achieved if  living in balance between material and spiritual. This is because human life does not just stop in the life of this world, but there is still a second life that will be faced by humanity in the hereafter, and well-being will be achieved with the truth is that people can balance the needs of the world and the hereafter, and that is what is taught in Islamic economics. Keywords: Islam, Economy, Prosperity. Tulisan ini bertujuan untuk menjelasakn tentang korelasi antara ekonomi  dan  Islam.  Islam  adalah  agama  yang  sempurna  yang mengatur segala hal dalam kehidupan ini, termasuk juga ekonomi. Hal ini terbukti dengan konsep kesejahteraan yang dipaparkan dalam al-Quran dan Sunnah. Pada dasarnya tujuan hidup setiap manusia adalah untuk mencapai kesejahteraan, meskipun manusia memaknai kesejahteraan dengan perspektif  yang berbeda-beda. Sebagian besar paham ekonomi menganggap bahwa kesejahteraan adalah kesejahteraan material duniawi. Namun Islam memaknai kesejahteraan dengan istilah  Falah  yang  berarti  kesejahteraan  holistik  dan  seimbang antara dimensi material dan spiritual. Al-Quran dan Sunnah telah mengajarkan bahwa kesejahteraan akan tercapai jika manusia menjalani hidup secara seimbang antara material dan

  14. Karakteristik Dan Spektrum Manajemen Pendidikan Islam

    OpenAIRE

    Jannah, Siti Raudhatul

    2013-01-01

    Artikel ini mendiskusikan mengenai manajemen pendidikan Islam, karakteristik, dan spektrumnya. Karakteristik manajemen pendidikan Islam harus didasarkan pada al-Qur'an dan Hadits serta pemikiran rasional yang telah diuji validitasnya. Spektrum manajemen pendidikan Islam meliputi lingkup manajemen personalia pendidikan Islam, manajemen peserta didik pendidikan Islam, maanjemen kurikulum pendidikan Islam, manajemen keuangan pendidikan Islam, manajemen sarana dan prasarana pendidikan Islam, mana...

  15. PRODUKSI NANOFIBER DAN APLIKASINYA DALAM PENGOLAHAN AIR

    OpenAIRE

    Krisnandika, Vania Elita

    2017-01-01

    Abstrak Kebutuhan air meningkat seiring meningkatnya jumlah penduduk dan taraf kehidupan masyarakat. Pembangunan yang dilakukan secara terus-menerus dan sangat cepat di Indonesia mengakibatkan penurunan kualitas air permukaan. Teknologi membran merupakan salah satu teknologi pengolahan air yang menghasilkan produk dengan kualitas tinggi. Membran berstruktur nano, khususnya nanofiber, saat ini menjadi perhatian karena menjawab kebutuhan teknologi filtrasi yang efektif dan hemat biaya. Pr...

  16. DETEKSI DAN MANAJEMEN REFEEDING SYNDROME

    Directory of Open Access Journals (Sweden)

    Darmadi Darmadi

    2012-09-01

    Full Text Available AbstrakRefeeding syndrome (RFS dideskripsikan sebagai perubahan biokimiawi, manifestasi klinis dan komplikasi sebagai konsekuensi pemberian nutrisi pada pasien kurang gizi. Refeeding syndrome ini menyebabkan dampak buruk dan kematian. Sindroma ini lebih sering terjadi pada kelompok risiko. Refeeding syndrome merupakan suatu sindroma yang sering tak terdiagnosis oleh karena itu perlu peningkatan pengetahuan dan kesadaran dari tenaga medis untuk mengurangi morbiditas dan mortalitas dari RFS. Karena alasan tersebut ditulis tinjauan pustaka ini yang akan membahas mengenai patofisiologi, faktor risiko, manifestasi klinis, dan manajemennya.Kata kunci : refeeding syndrome, kurang gizi, hipofosfatemia, dukungan nutrisiAbstractRefeeding syndrome (RFS describel as biochemical changes, clinical manifestations, and complications that can occur as a consequence of feeding a malnourished individual. RFS can result in serious harm and death. RFS more commonly occurs in at-risk populations. Increased awareness amongst healthcare professionals is likely to reduce morbidity and mortality. This review examines the pathophysiology, risk factors, clinical manifestations, and management of RFS.Key word : refeeding syndrome, malnourished, hypophosphatemia, nutritional support

  17. Fish and wildlife surveillance

    Energy Technology Data Exchange (ETDEWEB)

    Poston, T.M.

    1995-06-01

    This section of the 1994 Hanford Site Environmental Report summarizes the monitoring of radioactive contaminants in fish and wildlife species that inhabit the Colombia River and Hanford Site. Wildlife have access to areas of the Site containing radioactive contamination, and fish can be exposed to contamination in spring water entering the river along the shoreline. Therefore, samples are collected at various locations annually, generally during the hunting or fishing season, for selected species.

  18. Fish and wildlife surveillance

    International Nuclear Information System (INIS)

    Poston, T.M.

    1995-01-01

    This section of the 1994 Hanford Site Environmental Report summarizes the monitoring of radioactive contaminants in fish and wildlife species that inhabit the Colombia River and Hanford Site. Wildlife have access to areas of the Site containing radioactive contamination, and fish can be exposed to contamination in spring water entering the river along the shoreline. Therefore, samples are collected at various locations annually, generally during the hunting or fishing season, for selected species

  19. Islam, Media, dan Politik : Sebuah Perdebatan dan Kontempelasi Nilai Berdemokrasi

    Directory of Open Access Journals (Sweden)

    Idil Akbar

    2016-10-01

    Full Text Available Infact, press or media is one of pilar democracy. Media in democracy is a public sphere to communi-cation and makes relation, and a same time as public arena to gain information. But, media also could not release from streotypes and tendency that usually bring of interest, either the owner or ideology. It is meaning media can not released from perspective distorsion, values bias and take a side. Media also effective to become propaganda instrument for a political interest and an ideology. It is seen like how media in US, for example, stereotype of Islam in discourses contradiction and glob-al interest opposition. Then, Islam dominantly identified as doctrine teaches radicalism, fundamen-talism and terorism. Thus, it is often be justification for US to operate the military mission in the name of democracy. Tak dipungkiri pers atau media menjadi salah satu pilar bagi demokrasi. Media dalam demokrasi adalah sebagai ruang bagi publik berkomunikasi dan berinteraksi, sekaligus sarana publik mem-peroleh informasi tentang banyak hal. Namun, media juga tak bisa dilepaskan dari stereotip dan kecenderungan-kecenderungan, yang biasanya membawa kepentingan, baik pemilik maupun ide-ologi. Media karenanya tak lepas dari distorsi perspektif, bias nilai dan berpihak. Media juga efektif menjadi alat propaganda bagi sebuah kepentingan politik dan ideologi, seperti bagaimana media di negara Amerika, misalnya, menstereotipkan Islam dalam analisis pertentangan wacana dan kepent-ingan global. Sehingga, dominan Islam diidentikkan dengan sematan sebagai ajaran yang mengajarkan radikalisme, fundamentalisme dan terorisme. Karenanya pula seringkali menjadi alasan pembenar bagi Amerika untuk menjalankan misi militer atas nama demokrasi.

  20. Experimental observation of G banding verifying X-ray workers' chromosome translocation detected by FISH

    International Nuclear Information System (INIS)

    Sun Yuanming; Li Jin; Wang Qin; Tang Weisheng; Wang Zhiquan

    2002-01-01

    Objective: FISH is the most effective way of detecting chromosome aberration and many factors affect its accuracy. G-banding is used to verify the results of early X-ray workers' chromosome translocation examined by FISH. Methods: The chromosome translocations of early X-ray workers have been analysed by FISH (fluorescence in situ hybridization) and G-banding, yields of translocation treated with statistics. Results: The chromosome aberrations frequencies by tow methods are closely related. Conclusion: FISH is a feasible way to analyse chromosome aberrations of X-ray workers and reconstruct dose

  1. Nine New Fluorescent Probes

    Science.gov (United States)

    Lin, Tsung-I.; Jovanovic, Misa V.; Dowben, Robert M.

    1989-06-01

    Absorption and fluorescence spectroscopic studies are reported here for nine new fluorescent probes recently synthesized in our laboratories: four pyrene derivatives with substituents of (i) 1,3-diacetoxy-6,8-dichlorosulfonyl, (ii) 1,3-dihydroxy-6,8-disodiumsulfonate, (iii) 1,3-disodiumsulfonate, and (iv) l-ethoxy-3,6,8-trisodiumsulfonate groups, and five [7-julolidino] coumarin derivatives with substituents of (v) 3-carboxylate-4-methyl, (vi) 3- methylcarboxylate, (vii) 3-acetate-4-methyl, (viii) 3-propionate-4-methyl, and (ix) 3-sulfonate-4-methyl groups. Pyrene compounds i and ii and coumarin compounds v and vi exhibit interesting absorbance and fluorescence properties: their absorption maxima are red shifted compared to the parent compound to the blue-green region, and the band width broadens considerably. All four blue-absorbing dyes fluoresce intensely in the green region, and the two pyrene compounds emit at such long wavelengths without formation of excimers. The fluorescence properties of these compounds are quite environment-sensitive: considerable spectral shifts and fluorescence intensity changes have been observed in the pH range from 3 to 10 and in a wide variety of polar and hydrophobic solvents with vastly different dielectric constants. The high extinction and fluorescence quantum yield of these probes make them ideal fluorescent labeling reagents for proteins, antibodies, nucleic acids, and cellular organelles. The pH and hydrophobicity-dependent fluorescence changes can be utilized as optical pH and/or hydrophobicity indicators for mapping environmental difference in various cellular components in a single cell. Since all nine probes absorb in the UV, but emit at different wavelengths in the visible, these two groups of compounds offer an advantage of utilizing a single monochromatic light source (e.g., a nitrogen laser) to achieve multi-wavelength detection for flow cytometry application. As a first step to explore potential application in

  2. Musisi Sekuler dan Gerejawi

    Directory of Open Access Journals (Sweden)

    Benny Cristovel

    2007-12-01

    Full Text Available Menjadi seorang musisi tidak sekedar bermain musik atau menyanyi saja. Ada jenjang-jenjang yang akan dilewatidalam dunia musik, yang pada akhirnya setiap musisi dihadapkan kepada tanggung jawab dalam dunia tersebut. Di jaman ini, jika jenjang seorang musisi sudah tahap profesional, maka musik dan bisnis merupakan hal yang tidak terpisahkan. Jadi tidak heran jika ada banyak orang yang memimpikan untuk berkarier dalam bidang musik, bahkan telah men1adicita-cita sejak kecil.Jadi penting bagi musisi gerejawi benar-benar "mengkotakkan dirinya" terhadap persepsi yang dianut oleh musisi sekuler, bukan mencampurkannya. Hal ini sangat esensial, karena musisi gerejawi bukan melayani manusia melainkan oleh anugerah Tuhan dipercayakan pekerjaan ini. Dengan demikian pelayanan sebagai musisi gerejawi tidak tercemar oleh persepsi dari dunia musik sekuler. Selain itu musisi gerejawi dapat merefleksikan Tuhan yang disembahnya kepada dunia sekuler, karena tujuan dari semua yang dilakukannya adalah untuk kemuliaan nama Tuhan.

  3. ADAPTASI PERIKANAN PUKAT CINCIN DI LAUT JAWA DAN IMPLIKASINYA TERHADAP PENGELOLAAN

    Directory of Open Access Journals (Sweden)

    Suherman Banon Atmaja

    2014-11-01

    Full Text Available Usaha perikanan akan mengalami fluktuasi terkait dengan dinamika faktor alam, sedangkan nelayan mempunyai kemampuan yang lentur dan adaptif dalam kelangsungan usahanya. Tujuan utama makalah ini adalah membahas tentang kemampuan adaptasi perikanan purse seine untuk bertahan dari usaha perikanan tangkap. Pemetaan rekaman data sistem pemantauan kapal (VMS purse seine pelagis kecil tahun 2012 dilakukan berdasarkan kriteria kecepatan kapal nol adalah waktu melakukan aktifitas penangkapan. Daerah penangkapan perikanan pukat cincin pada saat ini telah menyebar semakin luas, tidak terbatas di wilayah teritorial dan perairan nusantara, tetapi sudah sampai ke wilayah Samudera (ZEEI. Jumlah hari laut pada perikanan cantrang kurang dari sebulan memperoleh rata – rata pendapatan nelayan ABK hampir dua kali lipat dari pada ABK pukat cincin yang beroperasi lebih dari sebulan. Rotasi penggunaan alat tangkap di Laut Jawa adalah suatu kondisi yang muncul ke permukaan sebagai sinyal pergeseran populasi ikan dari karakteristik sumber daya yang multi-spesies. Fenomena hasil tangkapan yang tidak tercatat dan dilaporkan menyebabkan pendugaan stok ikan dengan akurat yang rendah akan menimbulkan ketidakpastian dalam penyusunan rekomendasi. Tersedianya sistem pemantauan kapal perlu didukung oleh kegiatan validasi hasil tangkapan yang didaratkan, untuk itu sangat perlu meningkatkan keterlibatkan enumerator dan observer di atas kapal serta penguatan sistem log-book yang sedang berjalan.   The fishing business will fluctuate as a result of high connectivity with the dynamic and nature of environmental factors, in the other side, experience fishers have flexible and adaptive capabilities in order to sustain their business. The objective of this paper is to describe the purse seine fishery adaptability to survive from long term fishing. Plotting data on recorded vessel monitoring system (VMS of small pelagic purse seine in 2012 were applied based on the criteria of the

  4. Fish and hydroelectricity

    International Nuclear Information System (INIS)

    Zorpette, G.

    1990-01-01

    This paper reports on the problems that hydroelectric plants have regarding fish populations. The utilities that operate these plants are finding that accommodating migrating fish presents unique engineering challenges, not the least of which involves designing and building systems to protect fish species whose migratory behavior remains something of a mystery. Where such systems cannot be built, the status of hydroelectric dams may be in doubt, as is now the case with several dams in the United States. A further twist in some regions in the possibility that certain migratory fish will be declared threatened or endangered-a development that could wreak havoc on the hydroelectric energy supply in those regions

  5. Of Fish and Micrornas

    DEFF Research Database (Denmark)

    Bela-Ong, Dennis; Schyth, Brian Dall; Lorenzen, Niels

    Fish is an important small vertebrate multidisciplinary model for investigating various aspects of reproduction, development, disease (immunology, toxicology, carcinogenesis), and aging. It is also an important model for comparative and evolutionary studies because it represents the lower...... to the mechanisms of control of gene expression, impacting a broad range of biological processes. Thus far, >25, 000 miRNA sequences have been identified in 193 species, including fish. In fish, the interest on miRNAs started with the analysis of their expression and function during embryonic development. In our...... selection markers to identify disease-resistant fish....

  6. Intelligent Fish Freshness Assessment

    Directory of Open Access Journals (Sweden)

    Hamid Gholam Hosseini

    2008-01-01

    Full Text Available Fish species identification and automated fish freshness assessment play important roles in fishery industry applications. This paper describes a method based on support vector machines (SVMs to improve the performance of fish identification systems. The result is used for the assessment of fish freshness using artificial neural network (ANN. Identification of the fish species involves processing of the images of fish. The most efficient features were extracted and combined with the down-sampled version of the images to create a 1D input vector. Max-Win algorithm applied to the SVM-based classifiers has enhanced the reliability of sorting to 96.46%. The realisation of Cyranose 320 Electronic nose (E-nose, in order to evaluate the fish freshness in real-time, is experimented. Intelligent processing of the sensor patterns involves the use of a dedicated ANN for each species under study. The best estimation of freshness was provided by the most sensitive sensors. Data was collected from four selected species of fishes over a period of ten days. It was concluded that the performance can be increased using individual trained ANN for each specie. The proposed system has been successful in identifying the number of days after catching the fish with an accuracy of up to 91%.

  7. Characterization of Fish Skin Gelatin Yellowfin Tuna

    Directory of Open Access Journals (Sweden)

    Mala Nurilmala

    2017-08-01

    Full Text Available Gelatin is one of the products which become a necessity for various industries, i.e. food and non-food industries. The application of gelatin has been increasing year by year in Indonesia. However, there is nogelatin industry in Indonesia so far. Thus, it is necessary to find an alternative source of gelatin, especially from fishery by products.Thus, the purpose of this research was to extract fish skin gelatin of yellowfin tuna with temperature treatments (55, 65 and 75oC. In addition, the properties of resulted gelatin were determined including yield, pH, gel strength, viscosity, functional groups, molecular weight profiles, and amino acid composition. The extraction at 75oC was chosen as the best result. The yield was 17%; pH 5.3; gel strength 1789.55 gf, viscosity 104.2 Cp, respectively. There was functional group amide A, I, II, dan III. SDS-PAGE showed β, α1 dan α2 bands for tuna skin gelatin. In addition, the main amino acids were glycine and proline.

  8. Fish silage as feed ingredient for fish and livestock

    NARCIS (Netherlands)

    Rurangwa, E.; Vuuren, van A.M.; Poelman, M.

    2014-01-01

    The present report analyses through a literature review the potential of fish silage to valorise fish processing by-products into economically relevant protein sources for fish and livestock feed production in East Africa.

  9. Fish-allergic patients may be able to eat fish.

    Science.gov (United States)

    Mourad, Ahmad A; Bahna, Sami L

    2015-03-01

    Reported fish allergy prevalence varies widely, with an estimated prevalence of 0.2% in the general population. Sensitization to fish can occur by ingestion, skin contact or inhalation. The manifestations can be IgE or non-IgE mediated. Several fish allergens have been identified, with parvalbumins being the major allergen in various species. Allergenicity varies among fish species and is affected by processing or preparation methods. Adverse reactions after eating fish are often claimed to be 'allergy' but could be a reaction to hidden food allergen, fish parasite, fish toxins or histamine in spoiled fish. Identifying such causes would allow free consumption of fish. Correct diagnosis of fish allergy, including the specific species, might provide the patient with safe alternatives. Patients have been generally advised for strict universal avoidance of fish. However, testing with various fish species or preparations might identify one or more forms that can be tolerated.

  10. Essential Fish Habitat (EFH) Areas Protected From Fishing

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Designated Essential Fish Habitat (EFH) areas where fishing or the use of fishing gears has been restricted or modified in order to minimize the adverse effects of...

  11. PENGELOLAAN ZAKAT OLEH NEGARA DAN SWASTA Studi Efektifitas dan Efisiensi Pengelolaan Zakat Oleh BAZ Dan LAZ Kota Madiun

    Directory of Open Access Journals (Sweden)

    Atik Abidah

    2010-12-01

    Full Text Available Abstraks: Keberadaan lembaga zakat di Indonesia yang diakui oleh perundang-undangan ada dua, yaitu Badan Amil Zakat (BAZ dan Lembaga Amil Zakat (LAZ. BAZ adalah lembaga zakat yang dikelola oleh pemerintah, sedangkan LAZ adalah lembaga yang dikelola oleh masyarakat. Artikel ini merupakan merupakan hasil penelitian lapangan dengan menggunakan pendekatan kualitatif. Data digali langsung dari BAZ Kota Madiun dan LAZ dalam hal ini ada 2 lembaga, yaitu Lembaga Manajemen Infaq (LMI dan Baitul Maal Hidayatullah (BMH. Hasil penelitian BAZ lebih efektif dibanding LAZ, karena BAZ di bawah naungan Pemerintah Kota Madiun dan didukung dengan kebijakan dalam menjalankan progam kerjanya. Sedangkan pada LAZ (LMI dan BMH karena sifatnya mandiri, maka segala sesuatunya akan efektif jika mereka bekerja keras, dan itulah yang selama ini dilakukan oleh LMI dan BMH, sehingga sampai saat ini mereka masih eksis.

  12. INTERAKSI DAN HARMONI UMAT BERAGAMA

    Directory of Open Access Journals (Sweden)

    Joko Tri Haryanto

    2012-05-01

    Masyarakat Singkawang adalah masyarakat yang terdiri atas berbagai kelompok suku bangsa dan agama. Secara historis, masyarakat Singkawang mampu mem­pertahankan harmoni antar agama. Model interaksi sosial yang bersifat asosiatif tampaknya potensial untuk mendukung harmoni di dalam masyarakat. Ber­dasarkan paradigma fungsional-struktural, masyarakat diasumsikan sebagai sistem organik yang memiliki huungan antar bagiannya untuk mempertahankan masyarakat. Interaksi sosial di dalam masyarakat Singkawang dikembangkan melalui interaksi di dalam lingkup keluarga, lingkungan sekitar, aktifitas ekonomi, para pimpinan agama, dan hubungan di dalam lingkup budaya dan tradisi. Meskipun demikian, hubungan antar unsur masyarakat masih kosmopolitan, yang di dalamnya anggota masyarakat kurang aktif dalam mengembangkan harmoni masyarakat.

  13. FISH analysis for diagnostic evaluation of challenging melanocytic lesions.

    Science.gov (United States)

    Zimmermann, A K; Hirschmann, A; Pfeiffer, D; Paredes, B E; Diebold, J

    2010-09-01

    The differential diagnosis of malignant melanomas and atypical melanocytic nevi is still a diagnostic challenge. The currently accepted morphologic criteria show substantial interobserver variability, likewise immunohistochemical studies are often not able to discriminate these lesions reliably. Techniques that support diagnostic accuracy are of the greatest importance considering the growing incidence of malignant melanomas and their increase in younger patients. In this study we analyzed the feasibility of fluorescence in situ hybridization (FISH) analysis for the discrimination of malignant and benign melanocytic tumors. A panel of DNA probes was used to detect chromosomal aberrations of chromosomes 6 and 11. On a series of 5 clearly malignant and benign melanocytic tumors we confirmed the applicability of the test. Then we focused on examination of ambiguous melanocytic lesions, where atypical cells are often difficult to relocalize in the 4',6-Diamidino-2-phenylindol (DAPI)-fluorescence stain. FISH analyses were conducted on destained H&E-stained slides. By comparison of the DAPI-image with photos taken from the H&E stain, unambiguous assignment of the FISH results to the conspicuous groups of cells was possible. The results of FISH analysis were consistent with the conventional diagnosis in 11 of 14 small ambiguous lesions. Of the remaining 3 cases, 2 showed FISH-results close to the cut-off level. Comparison of FISH results on thin and thick sections revealed that the cut-off values have to be adapted for 2 microm destained sections. In conclusion, FISH analysis is a useful and applicable tool for assessment of even smallest melanocytic neoplasms, although there will remain unclear cases that cannot be solved even after additional FISH evaluation.

  14. 49 CFR 173.218 - Fish meal or fish scrap.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Fish meal or fish scrap. 173.218 Section 173.218... Fish meal or fish scrap. (a) Except as provided in Column (7) of the HMT in § 172.101 of this subchapter, fish meal or fish scrap, containing at least 6%, but not more than 12% water, is authorized for...

  15. ANALISIS KUALITAS LINGKUNGAN PERAIRAN BERDASARKAN KOMUNITAS MEIOBENTOS DAN KUALITAS SEDIMEN DI PANTAI DAN AREA PERTAMBAKAN, PESISIR SRIWULAN KABUPATEN DEMAK (The Quality Analysis of Aquatic Environment based on Meiobentos Community and Sediment Quality

    Directory of Open Access Journals (Sweden)

    Muhandis Sidqi

    2015-01-01

    Full Text Available ABSTRAK  Tujuan studi ini meliputi (1 menentukan kualitas lingkungan daerah pesisir dan tambak ikan, kualitas sedimen, dan komunitas meiobentos, (2 menemukan hubungan antara kualitas sedimen dan meiobentos, dan (3 menentukan tingkat produktivitas peikampungan tambak ikan berdasarkan jarak, tingkat polusi dan destruksi. Parameter kualitas air dianalisis menggunakan metode deskriptif-komparatif, sedangkan parameter sedimen dianalisis menggunakan PCA (Principal Component Analisys untuk menentukan distribusi spasial pada setiap stasiun pemantauan dan lapisan kedalaman sedimen. Komunitas meiobentos diperiksa dengan menggunakan CA (Factorial Correspondence Analysis untuk mendeteksi tingkat distribusi spasial yang juga berdasarkan stasiun pemantauan dan lapisan kedalaman sedimen. Data tersebut dianalisis menggunakan korelasi dan regresi untuk memahami pengaruh parameter bebas terhadap produktivitas tambak ikan. Kemudian tes statistik non parametric dari Kruskall Wallis digunakan untuk membedakan produktivitas pada 3 desa berdasarkan jarak terhadap sumber pencemaran dan tingkat destruksi tambak ikan. Penelitian ini menemukan bahwa nilai parameter kualitas air (muddy, TSS NH3, NO2, beyond threshold level, negative redox potential (Eh sediment value/reduction zone, and IMLP adalah moderat. Hal ini menunjukkan bahwa kondisi air di daerah penelitian tidak stabil. Penelitian juga menunjukkan nilai indeks diversitas yang rendah, dominasi organisme meiobentos tertentu, dan konformitas antar komunitas bentos. Hasil tes statistik Kruskall Wallis menunjukkan adanya signifikansi antara produktivitas tambak ikan dengan factor jarak dan tingkat destruksi diantara 3 desa dalam daerah penelitian yaitu Bedono, Sriwulan. Purwosari yaitu dengan nilai produktivitas 0.65,0.56, 0.41 ton/hektar/tahun.   ABSTRACT The objective of this study area to determine the environmental quality of coastal area and fish ponds which are on water quality, sediment quality, and Meiobentos

  16. Nanosecond fluorescence spectroscopy

    International Nuclear Information System (INIS)

    Leskovar, B.

    1985-03-01

    This article is a summary of a short course lecture given in conjunction with the 1984 Nuclear Science Symposium. Measuring systems for nanosecond fluorescence spectroscopy using single-photon counting techniques are presented. These involve systems based on relaxation-type spark gap light pulser and synchronously pumped mode-locked dye lasers. Furthermore, typical characteristics and optimization of operating conditions of the critical components responsible for the system time resolution are discussed. A short comparison of the most important deconvolution methods for numerical analysis of experimental data is given particularly with respect to the signal-to-noise ratio of the fluorescence signal. 22 refs., 8 figs

  17. Fluorescence uranium determination

    International Nuclear Information System (INIS)

    Fernandez Cellini, R.; Crus Castillo, F. de la; Barrera Pinero, R.

    1960-01-01

    An equipment for analysis of uranium by fluorescence was developed in order to determine it at such a low concentration that it can not be determined by the most sensible analytical methods. this new fluorimeter was adapted to measure the fluorescence emitted by the phosphorus sodium fluoride-sodium carbonate-potasium carbonate-uranyl, being excited by ultraviolet light of 3,650 A the intensity of the light emitted was measure with a photomultiplicator RCA 5819 and the adequate electronic equipment. (Author) 19 refs

  18. Anadromous fish behaviour important for fish passage

    International Nuclear Information System (INIS)

    Kynard, B.E.

    1993-01-01

    An understanding of the behavior of target fish species is necessary for proper design, location, and operation of a successful upstream or downstream fishway for anadromous migrants. Important fish behaviors are seasonal and daily timing of migration; rheotaxis and near field behavior; stimulus-response behavior; swimming capability; shoaling behavior; response to physical environmental factors such as illumination, sound, water depth, current velocity, and structure; response to chemicals; and response to biological factors such as competition for space and response to predators. The information on migrant fish behavior is reviewed, using examples from the literature on the behavior of eastern anadromous species, particularly Atlantic salmon (Salmo salar) and American shad (Alosa sapidissima). 87 refs

  19. ETIKA BISNIS AL-GHAZALI DAN ADAM SMITH DALAM PERSPEKTIF ILMU BISNIS DAN EKONOMI

    Directory of Open Access Journals (Sweden)

    AM. M. Hafidz MS.

    2013-05-01

    Full Text Available Penelitian ini adalah untuk mengeksplorasi: (1 pembangunan etika bisnis yang telah dirumuskan oleh al-Ghazali dan Adam Smith, (2 persamaan dan perbedaan antara dua etika bisnis pria, dan (3 relevansi bisnis mereka etika bisnis modern dunia dan ekonomi. Bisnis etika dibangun oleh al-Ghazali dan Smith di dataran praksis tidak jauh berbeda. Etika bisnis konstruksi dibangun oleh al-Ghazali didasarkan pada prinsip-prinsip seperti orientasi itikad baik tentang dunia dan akhirat, kejujuran, kepentingan pribadi dan social keseimbangan, dan perilaku / perbuatan yang tepat. Di sisi lain, etika bisnis konstruksi dibangun oleh Smith, berdasarkan keadilan, altruisme, keadilan dan liberal (kebebasan ekonomi. Baik etika bisnis yang diperkenalkan oleh kedua sangat relevan untuk menjadi digunakan sebagai bahan pokok acuan dalam etika bisnis modern.

  20. Pengembangan Sistem Otomatisasi AC dan Lampu Menggunakan Fuzzy dan Raspberry Pi

    Directory of Open Access Journals (Sweden)

    Rudy Ariyanto

    2017-11-01

    Full Text Available Otomatisasi AC dan lampu dilakukan untuk menghemat energi yang digunakan pada kehidupan sehari-hari. Dalam pengembangan otomatisasi AC dan lampu perlu menerapkan sebuah perangkat yang memiliki fungsi maksimal dengan harga yang minimal. Raspberry Pi merupakan perangkat atau modul dengan harga rendah yang mampu melakukan komunikasi wireless tanpa bantuan modul lain. Dalam pengembangan otomatisasi AC dan lampu juga diperlukan sebuah metode yang mampu melakukan kontrol terhadap nyala AC dan lampu. Penerapan metode fuzzy dapat dilakukan untuk menghimpun informasi keadaan ruang yang didapat dari sensor untuk menentukan nyala AC dan lampu secara otomatis. Oleh sebab itu pada penelitian ini mengusulkan pengembangan otomatisasi AC dan lampu menggunakan Raspberry Pi dan Fuzzy. Otomatisasi AC dan lampu menggunakan Raspberry Pi yang menerapkan metode Fuzzy dapat menghemat energi hingga 59,87% dalam hal lama waktu nyala AC dan 57,47% untuk lumenasi lampu

  1. Selective detection of heavy metal ions by calixarene-based fluorescent molecular sensors

    Science.gov (United States)

    Zhang, Haitao; Faye, Djibril; Zhang, Han; Lefevre, Jean-Pierre; Delaire, J. A.; Leray, Isabelle

    2012-06-01

    The synthesis, spectroscopic characterization and complexing properties of calixarene-based fluorescent sensors are reported. The calixarene bearing four dansyl fluorophores (Calix-DANS4) exhibits a very high affinity for the detection of lead. A fluorimetric micro-device based on the use of a Y-shape microchannel was developed and allows lead detection with a 5 ppb detection limit. For mercury detection, a fluorescent molecular sensor containing a calixarene anchored with four 8-quinolinoloxy groups (Calix-Q) has been synthesized. The absorption and fluorescence spectra of this sensor are sensitive to the presence of metal cations. An efficient fluorescence quenching is observed upon mercury complexation because of a photoinduced electron transfer from the fluorophore to the bound mercury. Calix-Q shows a high selectivity towards Hg2+ over interfering cations (Na+, K+, Ca2+, Cu2+, Zn2+, Cd2+ and Pb2+) and a 70 ppb sensitivity.

  2. Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway di Petshop "PetZone"

    Directory of Open Access Journals (Sweden)

    Siska Fadhilah Wati

    2013-09-01

    Full Text Available Dewasa ini teknologi informasi sangat membantu dalam dunia bisnis. Kemudahan yang ditawarkan akan menjaring lebih banyak konsumen. Namun, perusahan tingkat menengah seperti PetZone saat ini masih jarang yang menawarkan kemudahan berbelanja atau pemesanan layanan secara online. Oleh karena itu diperlukan sebuah sistem berbasis web di perusahaan PetZone yang dapat memudahkan pemilik dan karyawan dalam apenjualan, pelayanan, dan pemasaran, serta memudahkan pelanggan dalam jual-beli barang dan jasa. Atas dasar masalah tersebut dibangun Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway untuk menunjang proses bisnis yang ada. Sistem informasi dibangun menggunakan bahasa pemrograman PHP framework Codeigniter, javascript untuk tampilan yang dinamis, dan database MySQL. Proses pembuatan dan pengembangan Sistem Informasi Penjualan ini menggunakan metode air terjun. Metode air terjun meliputi kebutuhan pengguna, analisis, rancangan, implementasi dan pengujian. Pemodelan Sistem Informasi yang dibangun menggunakan metode berorientasi objek UML (Unified Modeling Language yang terdiri dari Use case  diagram, Class diagram dan Sequence diagram. Hasil pengujian Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway menunjukkan bahwa semua fitur yang terdapat baik dalam sistem informasi maupun SMS gateway dapat bekerja dengan baikdengan ratusan sample data, dan server SMS gateway dapat memproses lebih dari satu SMS secara bersamaan. Dalam perkembangan ke depannya nanti, Sistem Informasi Penjualan dan Pemesanan Layanan Berbasis Web dan SMS Gateway masih dapat dikembangkan lagi dengan menambah fitur-fitur pada SMS gateway sehingga lebih memudahkan konsumen.

  3. Efikasi Kendiri: Perbandingan Antara Islam dan Barat

    Directory of Open Access Journals (Sweden)

    Noornajihan, J.

    2014-12-01

    Full Text Available Terminologi efikasi kendiri (EK merupakan terminologi yang diperkenalkan dalam ilmu psikologi sekitar tahun 70-an. Namun, istilah ini masih kurang difahami oleh kebanyakan individu terutama mereka yang bukan berlatar belakangkan bidang psikologi. Oleh itu, satu keperluan hasil penulisan ini diketengahkan, memandangkan konsep ini penting dalam diri setiap individu bagi memacu kecemerlangan diri. Justeru, kertas kerja ini akan mengupas serba ringkas mengenai konsep EK dari perspektif Islam dan Barat, dengan melihat kepada beberapa titik persamaan dan perbezaan antara kedua-dua pandangan. Islam dan Barat bersetuju bahawa individu yang memiliki EK yang tinggi merupakan individu yang berfikiran positif, berani mengambil risiko dan tidak mudah berputus. Namun pandangan Islam terhadap konsep ini lebih luas, kerana Islam mengaitkan konsep ini dengan konsep tauhid uluhiyyah dan konsep manusia sebagai ahsan al-taqwim. Oleh demikian, EK dari perspektif Islam lebih bersifat kekal dan umum, di samping individu yang berefikasi tinggi menurut Islam ialah individu yang memiliki sifat sabar, syukur, redha dan redha.

  4. PERANCANGAN DAN PENGEMBANGAN PROTOTYPE SISTEM PARKIR

    Directory of Open Access Journals (Sweden)

    Indah Ayu Septriyaningrum

    2016-10-01

    Abstrak  Sistem parkir manual pada pelayanan parkir mall memerlukan pengelolaan dan pengembangan sistem yang lebih rumit dan jauh dari kata efisien. Informasi yang didapatkan pengelola parkir terkait kondisi parkir di lapangan setiap harinya masih kurang. Tujuan dari penelitian ini adalah merancang dan mengembangkan prototype sistem parkir. Metode pengembangan dan perancangan sistem pada penelitian ini adalah metode Waterfall. Hasil yang didapatkan adalah sistem ini dapat mendeteksi keberadaan mobil pada slot parkir yang tersedia dengan bantuan Intel Galileo Board Gen 2 sebagai kontroler, sensor LDR (Light Dependent Resistor dan sensor ultrasonik (PING sebagai alat bantu pendeteksi keberadaan mobil. Sistem dapat menampilkan aktifitas parkir pada aplikasi web seperti kondisi lahan parkir kosong, lahan terisi, waktu masuk, waktu keluar, lama parkir dan kapasitas parkir yang tersedia serta grafik pemakaian lahan parkir. Dapat disimpulkan bahwa perancangan dan pengembangan sistem berhasil diimplementasikan. Kata Kunci : Sistem parkir, waterfall, Intel Galileo Board Gen 2,sensor LDR (Light Dependent Resistor, sensor ultrasonik

  5. Evaluation of PMSG (Oodev® application on hepatosomatic and gonadosomatic index of snakehead fish

    Directory of Open Access Journals (Sweden)

    Rizal Akbar Hutagalung

    2015-10-01

    Full Text Available ABSTRACT Snakehead fish (Channa striata is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI and hepatosomatic index (HSI. The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish  ABSTRAK Ikan gabus (Channa striata merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS dan indeks hepatosomatik (IHS. Induk ikan gabus yang digunakan berukuran 30−40 cm

  6. PARASITES OF FISH

    Science.gov (United States)

    The intent of this chapter is to describe the parasites of importance to fishes maintained and used in laboratory settings. In contrast to the frist edition, the focus will be only on those parasites that pose a serious threat to or are common in fishes held in these confined en...

  7. Enzymes in Fermented Fish.

    Science.gov (United States)

    Giyatmi; Irianto, H E

    Fermented fish products are very popular particularly in Southeast Asian countries. These products have unique characteristics, especially in terms of aroma, flavor, and texture developing during fermentation process. Proteolytic enzymes have a main role in hydrolyzing protein into simpler compounds. Fermentation process of fish relies both on naturally occurring enzymes (in the muscle or the intestinal tract) as well as bacteria. Fermented fish products processed using the whole fish show a different characteristic compared to those prepared from headed and gutted fish. Endogenous enzymes like trypsin, chymotrypsin, elastase, and aminopeptidase are the most involved in the fermentation process. Muscle tissue enzymes like cathepsins, peptidases, transaminases, amidases, amino acid decarboxylases, glutamic dehydrogenases, and related enzymes may also play a role in fish fermentation. Due to the decreased bacterial number during fermentation, contribution of microbial enzymes to proteolysis may be expected prior to salting of fish. Commercial enzymes are supplemented during processing for specific purposes, such as quality improvement and process acceleration. In the case of fish sauce, efforts to accelerate fermentation process and to improve product quality have been studied by addition of enzymes such as papain, bromelain, trypsin, pepsin, and chymotrypsin. © 2017 Elsevier Inc. All rights reserved.

  8. Perspectives on fish impingement

    International Nuclear Information System (INIS)

    Sharma, R.K.

    1977-01-01

    Data on fish impingement and related parameters are being gathered at a large number of power stations throughout the country at substantial monetary and manpower costs. A national survey of fish impingement at power plants was conducted and much of the information compiled in a standardized format--an effort that we think will aid in planning improvements in the design, siting, and operation of the cooling-water intakes. This paper examines the objectives of the fish impingement studies, monitoring programs, variables affecting fish impingement, siting and design criteria, state-of-the-art of screening systems, and suggestions for meeting 316(b) requirements. It also discusses where the emphasis should be placed in future fish-impingement related activities

  9. Detection of Fish Bones in Cod Fillets by UV Illumination.

    Science.gov (United States)

    Wang, Sheng; Nian, Rui; Cao, Limin; Sui, Jianxin; Lin, Hong

    2015-07-01

    The presence of fish bones is now regarded as an important hazard in fishery products, and there is increasing demand for new analytical techniques to control it more effectively. Here, the fluorescent properties of cod bones under UV illumination were investigated, and the maximal wavelengths for excitation and emission were determined to be 320 nm and 515 nm, respectively, demonstrating significantly different fluorescence characteristics and much higher fluorescence intensity compared to those of fillet muscles. Based on the results, UV fluorescence-assisted candling for the detection of bones in fishery products was developed for the first time. Using cod fillets as samples, the detection ratio of this technique was calculated as 90.86%, significantly higher than that of traditional candling under daylight (76.78%). Moreover, the working efficiency of the new technique was about 26% higher than that of the traditional method. A UV fluorescence imaging framework was also developed, and a method for automatic identification of the fish bones in the cod fillets based on the linear discriminant analysis proposed by Fisher was preliminarily realized, but the detection ratio was demonstrated to be relatively poor compared to those of candling techniques. These results allow us to suggest UV-based methods as new and promising approaches for routine monitoring of bones in fishery products.

  10. Monitoring by fluorescence measurements

    International Nuclear Information System (INIS)

    Malcolme-Lawes, D.J.; Gifford, L.A.

    1981-01-01

    A fluorimetric detector is described in which the fluorescence excitation source may be 3 H, 14 C, 35 S, 147 Pm or 63 Ni. Such a detector can be adapted for use with flowing liquid systems especially liquid chromatography systems. (U.K.)

  11. Fluorescence lifetime based bioassays

    Science.gov (United States)

    Meyer-Almes, Franz-Josef

    2017-12-01

    Fluorescence lifetime (FLT) is a robust intrinsic property and material constant of fluorescent matter. Measuring this important physical indicator has evolved from a laboratory curiosity to a powerful and established technique for a variety of applications in drug discovery, medical diagnostics and basic biological research. This distinct trend was mainly driven by improved and meanwhile affordable laser and detection instrumentation on the one hand, and the development of suitable FLT probes and biological assays on the other. In this process two essential working approaches emerged. The first one is primarily focused on high throughput applications employing biochemical in vitro assays with no requirement for high spatial resolution. The second even more dynamic trend is the significant expansion of assay methods combining highly time and spatially resolved fluorescence data by fluorescence lifetime imaging. The latter approach is currently pursued to enable not only the investigation of immortal tumor cell lines, but also specific tissues or even organs in living animals. This review tries to give an actual overview about the current status of FLT based bioassays and the wide range of application opportunities in biomedical and life science areas. In addition, future trends of FLT technologies will be discussed.

  12. Fluorescent Lamp Replacement Study

    Science.gov (United States)

    2017-07-01

    not be cited for purposes of advertisement. DISPOSITION INSTRUCTIONS: Destroy this document when no longer needed. Do not return to the... recycling , and can be disposed safely in a landfill. (2) LEDs offer reduced maintenance costs and fewer bulb replacements, significantly reducing... recycling . Several fixtures, ballasts and energy efficient fluorescent bulbs that were determined to be in pristine condition were returned to ATC

  13. Efektivitas Weblog dan Facebook Terintegrasi untuk Pembelajaran Virtual

    OpenAIRE

    Hartono

    2014-01-01

    Abstrak: Saat ini, banyak mahasiswa menghabiskan waktu mereka di internet. Mereka melakukan kegiatan seperti membuat blog, mengunduh, mengunggah, chatting, dan berinteraksi melalui facebook. Mahasiswa sekarang ini hidup dengan teknologi. Penelitian menunjukkan bahwa weblog dan facebook berpotensi mendukung belajar dan mengajar. Guru dan pendidik menggunakan kegiatan tersebut untuk meningkatkan kualitas belajar. Penelitian ini bertujuan untuk mengetahui efektivitas weblog dan facebook ketika d...

  14. Fish allergy in childhood.

    Science.gov (United States)

    Pascual, Cristina Y; Reche, Marta; Fiandor, Ana; Valbuena, Teresa; Cuevas, Teresa; Esteban, Manuel Martin

    2008-11-01

    Fish and its derived products play an important role in human nutrition, but they may also be a potent food allergen. Fish can be an ingested, contact, and inhalant allergen. Gad c I, a Parvalbumin, the major allergen in codfish, is considered as fish and amphibian pan-allergen. Prevalence of fish allergy appears to depend on the amount of fish eaten in the local diet. In Europe, the highest consumption occurs in Scandinavian countries, Spain and Portugal. In Spain, fish is the third most frequent allergen in children under 2 yr of age after egg and cow's milk. An adverse reaction to fish may be of non-allergic origin, due to food contamination or newly formed toxic products, but the most frequent type of adverse reactions to fish are immunologic-mediated reactions (allergic reactions). Such allergic reactions may be both IgE-mediated and non-IgE-mediated. Most cases are IgE-mediated, due to ingestion or contact with fish or as a result of inhalation of cooking vapors. Some children develop non-IgE-mediated type allergies such as food protein induced enterocolitis syndrome. The clinical symptoms related to IgE-mediated fish allergy are most frequently acute urticaria and angioedema as well as mild oral symptoms, worsening of atopic dermatitis, respiratory symptoms such as rhinitis or asthma, and gastrointestinal symptoms such as nausea and vomiting. Anaphylaxis may also occur. Among all the species studied, those from the Tunidae and Xiphiidae families appear to be the least allergenic.

  15. Rapid method for identification of transgenic fish zygosity

    Directory of Open Access Journals (Sweden)

    . Alimuddin

    2007-07-01

    Full Text Available Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart.  This method needs relatively large amounts of fish material and is time- and labor-intensive. New approaches addressing this problem could be of great help for fish biotechnologists.  In this experiment, we applied a quantitative real-time PCR (qr-PCR method to analyze zygosity in a stable line of transgenic zebrafish (Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene. The qr-PCR was performed using iQ SYBR Green Supermix in the iCycler iQ Real-time PCR Detection System (Bio-Rad Laboratories, USA.  Data were analyzed using the comparative cycle threshold method.  The results demonstrated a clear-cut identification of all transgenic fish (n=20 classified as a homozygous or heterozygous.  Mating of those fish with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws. Thus, we found that the qTR-PCR to be effective for a rapid and precise determination of zygosity in transgenic fish. This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact. Keywords: quantitative real-time PCR; zygosity; transgenic fish; mass production   ABSTRAK Identifikasi sigositas ikan transgenik biasanya dilakukan menggunakan analisa PCR dengan cetakan DNA genomik yang diekstraksi dari jaringan ikan hasil persilangan antara ikan transgenik dan ikan normal.   Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga.  Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan.  Pada penelitian ini, kami menggunakan metode PCR real-time kuantitatif (krt-PCR untuk

  16. Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy

    Czech Academy of Sciences Publication Activity Database

    Macháň, Radek; Kapusta, Peter; Hof, Martin

    Roč. 406 , č. 20 (2014), s. 4797-4813 ISSN 1618-2642 R&D Projects: GA ČR GBP208/12/G016 Institutional support: RVO:61388955 Keywords : Filtered fluorescence correlation spectroscopy * Fluorescence lifetime correlation spectroscopy * Fluorescence spectral correlation spectroscopy Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.436, year: 2014

  17. Why fishing magnifies fluctuations in fish abundance.

    Science.gov (United States)

    Anderson, Christian N K; Hsieh, Chih-hao; Sandin, Stuart A; Hewitt, Roger; Hollowed, Anne; Beddington, John; May, Robert M; Sugihara, George

    2008-04-17

    It is now clear that fished populations can fluctuate more than unharvested stocks. However, it is not clear why. Here we distinguish among three major competing mechanisms for this phenomenon, by using the 50-year California Cooperative Oceanic Fisheries Investigations (CalCOFI) larval fish record. First, variable fishing pressure directly increases variability in exploited populations. Second, commercial fishing can decrease the average body size and age of a stock, causing the truncated population to track environmental fluctuations directly. Third, age-truncated or juvenescent populations have increasingly unstable population dynamics because of changing demographic parameters such as intrinsic growth rates. We find no evidence for the first hypothesis, limited evidence for the second and strong evidence for the third. Therefore, in California Current fisheries, increased temporal variability in the population does not arise from variable exploitation, nor does it reflect direct environmental tracking. More fundamentally, it arises from increased instability in dynamics. This finding has implications for resource management as an empirical example of how selective harvesting can alter the basic dynamics of exploited populations, and lead to unstable booms and busts that can precede systematic declines in stock levels.

  18. Optimization of three FISH procedures for in situ detection of anaerobic ammonium oxidizing bacteria in biological wastewater treatment.

    Science.gov (United States)

    Pavlekovic, Marko; Schmid, Markus C; Schmider-Poignee, Nadja; Spring, Stefan; Pilhofer, Martin; Gaul, Tobias; Fiandaca, Mark; Löffler, Frank E; Jetten, Mike; Schleifer, K-H; Lee, Natuschka M

    2009-08-01

    Fluorescence in situ hybridization (FISH) using fluorochrome-labeled DNA oligonucleotide probes has been successfully applied for in situ detection of anaerobic ammonium oxidizing (anammox) bacteria. However, application of the standard FISH protocols to visualize anammox bacteria in biofilms from a laboratory-scale wastewater reactor produced only weak signals. Increased signal intensity was achieved either by modifying the standard FISH protocol, using peptide nucleic acid probes (PNA FISH), or applying horse radish peroxidase- (HRP-) labeled probes and subsequent catalyzed reporter deposition (CARD-FISH). A comparative analysis using anammox biofilm samples and suspended anammox biomass from different laboratory wastewater bioreactors revealed that the modified standard FISH protocol and the PNA FISH probes produced equally strong fluorescence signals on suspended biomass, but only weak signals were obtained with the biofilm samples. The probe signal intensities in the biofilm samples could be enhanced by enzymatic pre-treatment of fixed cells, and by increasing the hybridization time of the PNA FISH protocol. CARD-FISH always produced up to four-fold stronger fluorescent signals but unspecific fluorescence signals, likely caused by endogenous peroxidases as reported in several previous studies, compromised the results. Interference of the development of fluorescence intensity with endogenous peroxidases was also observed in cells of aerobic ammonium oxidizers like Nitrosomonas europea, and sulfate-reducers like Desulfobacter postgatei. Interestingly, no interference was observed with other peroxidase-positive microorganisms, suggesting that CARD-FISH is not only compromised by the mere presence of peroxidases. Pre-treatment of cells to inactivate peroxidase with HCl or autoclavation/pasteurization failed to inactive peroxidases, but H(2)O(2) significantly reduced endogenous peroxidase activity. However, for optimal inactivation, different H(2)O(2

  19. Lõuka tuuliku restaureerimine / Dan Lukas

    Index Scriptorium Estoniae

    Lukas, Dan

    2005-01-01

    Hiiumaal Pühalepa vallas Vahtrepa külas Lõuka talu maadel asuva pukktuuliku ajaloost, restaureerimise käigust. Teostus: Dan Lukas. Muinsuskaitseline järelevalve: Tõnu Sepp, tema kommentaar. Ill.:pukktuuliku lõiked ja korruste plaanid, 7 värv. fotot

  20. Internal Control: Peran Dan Perkembangannya

    OpenAIRE

    Murtin, Alek

    2000-01-01

    Perhatian terhadap pentingnya internal control dalam organisasi berjalan dari waktu ke waktu untuk meningkatkan fungsi internal control yang disesuaikan dengan perkembangan lingkungan organisasi, dimulai dengan adanya internal check, kemudian internal control system, internal control structure dan terakhir adalah internal control-integrated framework. Internal control system sempat berkembang beberapa tahun di Indonesia yang selanjutnya digantikan oleh internal control structure yang digunaka...

  1. Asuransi Takaful: Prospek Dan Tantangannya

    OpenAIRE

    Sobirin, Achmad; Muhtar, Muchsin

    1994-01-01

    Dewasa ini perhatian terhadap nilai-nilai syariah Islam terhadap muamalah khususnya di bidang ekonomi menjadi besar. Lembaga perekonomian yang terjalin dengan pranata keagamaan makin menggejala dimana-mana dan kini sedang tumbuh mencari bentuk untuk dapat diterima di tengah-tengah masyarakat.

  2. Who's who in fluorescence 2008

    CERN Document Server

    Geddes, Chris D

    2008-01-01

    The Journal of Fluorescence's sixth Who's Who directory publishes the names, contact details, specialty keywords, and a brief description of scientists employing fluorescence methodology and instrumentation in their working lives. This is a unique reference.

  3. STRUKTUR KOMUNITAS DAN RELUNG MAKANAN IKAN PASCA INTRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus DI WADUK MALAHAYU, KABUPATEN BREBES

    Directory of Open Access Journals (Sweden)

    Kunto Purnomo

    2016-06-01

    Full Text Available Studi tentang struktur komunitas ikan dan pembagian sumber daya pakan ikan pasca introduksi ikan patin siam (Pangasianodon hypophthalmus di Waduk Malahayu (620 ha dilakukan dengan tujuan untuk mengetahui komposisi jenis ikan, preferensi makanan, dan luas relung tiap jenis ikan. Penelitian dilaksanakan dengan menggunakan metode survei pada bulan Agustus sampai Nopember 2009 dan bulan Maret sampai Oktober 2010. Hasil penelitian menunjukkan bahwa struktur komunitas ikan terdiri atas 13 jenis ikan, yang didominansi oleh ikan nila (Oreochromis niloticus, udang (Macrobrachium sp., dan gabus (Channa striata. Jenis-jenis sumber daya pakan yang dimanfaatkan oleh ikan adalah fitoplankton (20%, detritus (19%, zooplankton (17%, insekta (11%, tumbuhan air (9%, ikan (9%, udang (9%, dan moluska (6%. Ikan patin siam, mujair (Oreochromis mossambicus, dan beunteur (Puntius binotatus bersifat generalis karena mampu memanfaatkan semua sumber daya pakan alami yang tersedia. Ikan sili (Macrognathus aculeatus dan keting (Mystus nigriceps lebih bersifat spesialis karena hanya memanfaatkan insekta sebagai makanan utamanya. Peluang kompetisi antara ikan patin siam (diintroduksikan tahun 2009 dan ikan nila relatif kecil sebab sumber daya pakan utamanya berbeda, yaitu ikan patin siam memanfaatkan moluska sebagai makanan utamanya sedangkan ikan nila sebagian memanfaatkan fitoplankton. Hasil tangkapan ikan di waduk ini berkisar antara 34,3-1.323,1 ton/tahun dengan rata-rata 157,3 ton/tahun.   Malahayu Reservoir located in Brebes Regency, was impounded in 1930, with a surface area of 620 hectares, a mean water depth of 8 m. Its main function are flood control and irrigation. Study on fish community structure and food resource partitioning of fishes in Malahayu Reservoir were conducted from August to November 2009 and March to October 2010. The aim of the study was to evaluate the existing condition of fish resources, with emphasis on species composition, food preferency, and

  4. KETENTUAN TENTANG STANDAR PELAYANAN MINIMAL BIDANG KELUARGA BERENCANA DAN KELUARGA SEJAHTERA BERDASARKAN ASAS PERIKEMANUSIAAN DAN HAK ASASI MANUSIA

    Directory of Open Access Journals (Sweden)

    . Riyanti

    2017-01-01

    Hasil penelitian, ketentuan tentang standar pelayanan minimal bidang keluarga berencana, keluarga sejahtera dan asas perikemanusiaan dengan hak asasi manusia sangat berhubungan keterbatasan lingkup pelayanan dan standar pelaksanaan Komunikasi Informasi dan Edukasi yang tidak jelasberdampak pada tidak dipenuhinya hak asasi manusia untuk mendapatkan kesetaraan dan kebebasan dalam pelayanan keluarga berencana dan keluarga sejahtera.

  5. KINERJA DAN EFISIENSI BANK PEMERINTAH (BUMN DAN BUSN YANG GO PUBLIK DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Sugeng Haryanto

    2012-06-01

    Full Text Available Penelitian ini menganalisis kinerja dan tingkat efisiensi bank-bank BUMN dan BUSN yang go Publik di Bursa Efek Indonesia.  Sample penelitian ini mengambil  tiga bank BUMN Bank BNI 46, Bank Mandiri dan Bank BRI  dan tiga bank BUSN (Bank BCA, Bank Niaga dan Bank Panin dengan periode analisis tahun 2005-2011. Varibael yang digunakan meliputi ROA, ROE, LAR. LDR, NPL dan BOPO. Tujuan dari penelitian ini adalah melihat dan menganalisis perbedaan kinerja antara Bank BUMN dan BUSN yang go public di Bursa Efek Indonesia tahun 2005-2011.  Pendekatan pengukuran kinerja yang digunakan adalah Return on Asset (ROA, Return on Equity (ROE dan Loan to Deposit Ratio (LDR, Loan to Asset Ratio (LAR,  dan efisiensi bank. Hasil dari penelitian menunjukkan bahwa 1 Bank-bank nasional, baik itu bank BUMN maupun BUSN menunjukkan kinerja yang semakin baik, 2 tidak terdapat perbedaan yang signifikan antara kinerja bank BUMN dan BUSN untuk variabel ROA, ROE, LAR, LDR, dan BOPO sedangkan variabel NPL yang merupakan indikator risiko kredit menunjukkan adanya perbedaan yang signifikan antara Bank BUMN dan BUSN

  6. PEMBINAAN DAN PENGEMBANGAN USAHA KECIL

    Directory of Open Access Journals (Sweden)

    Sentot Harman Glendoh

    2001-01-01

    Full Text Available Small business as an economic activity of society on a small scale, has a central role in the economy of Indonesia. Although the economic crisis has created a great disturbance in the live of large and middle-size business, apparently small business continues to function well in the lower levels of the economy. The main role of small business is: 1 use excess labor; 2 as a producer of goods and services at reachable prices for the lower economic levels of society; 3 as a potential producer of foreign exchange because of the success of this type of industry in producing non-oil commodities for export. Remembering that the role of small business is large and has great influence on the lower economic levels of society, it is quite necessary to pay attention to the development of this sector. Abstract in Bahasa Indonesia : Usaha kecil sebagai kegiatan ekonomi rakyat berskala kecil memiliki peran sentral dalam perekonomian Indonesia. Walaupun krisis ekonomi telah memporakporandakan kehidupan bidang usaha besar dan menengah, ternyata usaha kecil tetap tegar dan berjalan marak dikawasan kehidupan ekonomi tingkat bawah. Peran pokok usaha kecil ini adalah: (1 sebagai penyerap tenaga kerja, (2 sebagai penghasil barang dan jasa pada tingkat harga yang terjangkau bagi kebutuhan rakyat banyak yang berpenghasilan rendah, (3 sebagai penghasil devisa negara yang potensial kerena keberhasilannya dalam meproduksi komoditi ekspor non migas. Mengingat peran usaha kecil tersebut sangat besar andilnya bagi negara dan masyarakat kecil dilapisan bawah, maka pembinaan dan pengembangannya sangat perlu diperhatikan. Kata kunci: pembinaan, pengembangan, usaha kecil.

  7. AGROFORESTRI UNTUK ADAPTASI DAN MITIGASI PERUBAHAN IKLIM

    Directory of Open Access Journals (Sweden)

    Tigor Butarbutar

    2017-01-01

    Full Text Available Hilangnya tutupan lahan hutan karena konversi hutan untuk pemukiman, perkebunan, pertanian dan kebutuhan untuk pembangunan di sektor lain, telah menyebabkan perubahan pola cuaca/iklim di berbagai tempat. Perubahan iklim dapat diantisipasi dengan mitigasi dan adaptasi. Mitigasi berarti usahausaha pencegahan yang perlu dilakukan, sedangkan adaptasi merupakan kegiatan-kegiatan penyesuaian yang perlu dilakukan untuk dapat hidup dan bertahan dan meningkatkan ketahanan, kelenturan dan mengarahkemigrasikarenakondisiiklimyangberbeda.Agroforestridapatmemitigasidanmengadaptasi perubahaniklim dengan alasan-alasansebagaiberikut: aPencampuranjenis pohonpenghasilkayu,buah dan lain-lain, karena campuran jenis lebih baik dari tanaman murni; b Pencampuran jenis yang didasarkan pada sifat toleransi ( dan , sehingga akan memanfaatkan seluruh cahaya untuk fotosintesis; c Pencampuran perbedaan umur; d Pencampuran berdasarkan perbedaan waktu pemanenan; e Penggabungan nilai ekonomi, sosial dan budaya sehingga perubahan vegetasi dapat berjalan seiring dengan perubahan sosial dan budaya secara berangsur yang dapat disesuaikan dengan perubahan iklim; dan f Dapat digunakan sebagai model untuk memfasilitasi perubahan kelompok vegetasi menjadi kelompok yang baru (adaptasi, seperti teori perubahan vegetasi melalui perladangan berpindah-pindahyangteratur.

  8. Synthesis and Fluorescence Spectra of Triazolylcoumarin Fluorescent Dyes

    Institute of Scientific and Technical Information of China (English)

    PENG Xian-fu; LI Hong-qi

    2009-01-01

    Much attention is devoted to fluorescent dyes especially those with potential in versatile applications. Reactions under "click" conditions between nonfluorescent 3 - azidocoumarins and terminal alkynes produced 3 -(1, 2, 3- triazol- 1 - yl)cournarins, a novel type of fluorescent dyes with intense fluorescence. The structures of the new coumarins were characterized by 1H NMR, MS, and IR spectra. Fluorescence spectra measurement demonstrated excellent fluorescence performance of the triazolylcoumarins and this click reaction is a promising candidate for bioconjugation and bioimaging applications since both azide and alkynes are quite inert to biological systems.

  9. Fluorescence spectroscopy of dental calculus

    International Nuclear Information System (INIS)

    Bakhmutov, D; Gonchukov, S; Sukhinina, A

    2010-01-01

    The aim of the present study was to investigate the fluorescence properties of dental calculus in comparison with the properties of adjacent unaffected tooth structure using both lasers and LEDs in the UV-visible range for fluorescence excitation. The influence of calculus color on the informative signal is demonstrated. The optimal spectral bands of excitation and registration of the fluorescence are determined

  10. Fluorescence spectroscopy of dental calculus

    Science.gov (United States)

    Bakhmutov, D.; Gonchukov, S.; Sukhinina, A.

    2010-05-01

    The aim of the present study was to investigate the fluorescence properties of dental calculus in comparison with the properties of adjacent unaffected tooth structure using both lasers and LEDs in the UV-visible range for fluorescence excitation. The influence of calculus color on the informative signal is demonstrated. The optimal spectral bands of excitation and registration of the fluorescence are determined.

  11. Fluorescence Imaging Reveals Surface Contamination

    Science.gov (United States)

    Schirato, Richard; Polichar, Raulf

    1992-01-01

    In technique to detect surface contamination, object inspected illuminated by ultraviolet light to make contaminants fluoresce; low-light-level video camera views fluorescence. Image-processing techniques quantify distribution of contaminants. If fluorescence of material expected to contaminate surface is not intense, tagged with low concentration of dye.

  12. Who's who in fluorescence 2005

    CERN Document Server

    Geddes, Chris D

    2006-01-01

    The Journal of Fluorescence's third Who's Who directory publishes the names, contact details, specialty keywords, photographs, and a brief description of scientists employing fluorescence methodology and instrumentation in their working livesThe directory provides company contact details with a brief list of fluorescence-related products.

  13. INDUSTRIALISASI DAN TANTANGANNYA PADA SEKTOR PENDIDIKAN

    Directory of Open Access Journals (Sweden)

    Arif Unwanullah

    2015-10-01

    Full Text Available Abstrak: Industrialisasi dan Tantangannya pada Sektor Pendidikan. Sumbangan pendidikan dalam perubahan dan pembangunan masa lalu telah bergeser dengan kemajuan teknoekonomi dan komunikasi. Perubahan yang terjadi telah menggeser tatanan kehidupan dan pandangan masyarakat. Materialisme, kapitalisme, efisiensi, dan efektivitas telah menjadi tujuan dan semangat hidup. Pergeseran pandangan masyarakat telah mengubah pula pandangan keberhasilan dan mutu pendidikan, di mana pendidikan diukur dari keberhasilan dalam keterserapan lulusan dalam dunia kerja, oleh karenanya pendidikan dianalisis dari karakteristik sebagai investasi (capital-investment. Pergeseran makna dan tanggung jawab pendidikan mendorong dunia pendidikan melakukan pembaruan dengan alternatif: membangun pembaruan penalaran warganya menuju pemerdekaan dan pendewasaan, pendidikan dilaksanakan secara komprehensif dan bekerjasama dengan semua pihak secara kemitraan, dan membangun visi pendidikan secara komprehensif dan simultan dengan semua pihak. Kata kunci: perubahan sosial, materialistik, modernisasi dan kapitalis Abstract: Industrialization and its education Sector challenges. Contribution of education has shifted with the progress of technology, economy, and communication. The changes have shifted the society's views. Materialism, capitalism, efficiency, and effectiveness have become of interest and enthusiasm for life. The shift has changed society's view, i.e. the view of success and quality of education, in which education is measured from a rate of the absorption of graduates into labor market, therefore education is analyzed from the characteristics of an investment (capital-investment. A shift in meaning and responsibility of education encourages the education sector to create reformation through these following alternatives: creating new thoughts towards liberation and maturation, implementing education comprehensively and cooperating with all parties in partnership, and creating

  14. Why do fish school?

    Institute of Scientific and Technical Information of China (English)

    Matz LARSSON

    2012-01-01

    Synchronized movements (schooling) emit complex and overlapping sound and pressure curves that might confuse the inner ear and lateral line organ (LLO) of a predator.Moreover,prey-fish moving close to each other may blur the electro-sensory perception of predators.The aim of this review is to explore mechanisms associated with synchronous swimming that may have contributed to increased adaptation and as a consequence may have influenced the evolution of schooling.The evolutionary development of the inner ear and the LLO increased the capacity to detect potential prey,possibly leading to an increased potential for cannibalism in the shoal,but also helped small fish to avoid joining larger fish,resulting in size homogeneity and,accordingly,an increased capacity for moving in synchrony.Water-movements and incidental sound produced as by-product of locomotion (ISOL) may provide fish with potentially useful information during swimming,such as neighbour body-size,speed,and location.When many fish move close to one another ISOL will be energetic and complex.Quiet intervals will be few.Fish moving in synchrony will have the capacity to discontinue movements simultaneously,providing relatively quiet intervals to allow the reception of potentially critical environmental signals.Besides,synchronized movements may facilitate auditory grouping of ISOL.Turning preference bias,well-functioning sense organs,good health,and skillful motor performance might be important to achieving an appropriate distance to school neighbors und aid the individual fish in reducing time spent in the comparatively less safe school periphery.Turning preferences in ancestral fish shoals might have helped fish to maintain groups and stay in formarion,reinforcing aforementioned predator confusion mechanisms,which possibly played a role in the lateralization of the vertebrate brain [Current Zoology 58 (1):116-128,2012].

  15. Technical Resources for Fish and Shellfish Consumption

    Science.gov (United States)

    Information on ways to develop local fish advisories, access national state and local fish advisories, obtain information on fish tissue contamination and fish tissue studies, and access information on fish consumption and human health.

  16. Which Fish Should I Eat? Perspectives Influencing Fish Consumption Choices

    Science.gov (United States)

    Choi, Anna L.; Karagas, Margaret R.; Mariën, Koenraad; Rheinberger, Christoph M.; Schoeny, Rita; Sunderland, Elsie; Korrick, Susan

    2012-01-01

    Background: Diverse perspectives have influenced fish consumption choices. Objectives: We summarized the issue of fish consumption choice from toxicological, nutritional, ecological, and economic points of view; identified areas of overlap and disagreement among these viewpoints; and reviewed effects of previous fish consumption advisories. Methods: We reviewed published scientific literature, public health guidelines, and advisories related to fish consumption, focusing on advisories targeted at U.S. populations. However, our conclusions apply to groups having similar fish consumption patterns. Discussion: There are many possible combinations of matters related to fish consumption, but few, if any, fish consumption patterns optimize all domains. Fish provides a rich source of protein and other nutrients, but because of contamination by methylmercury and other toxicants, higher fish intake often leads to greater toxicant exposure. Furthermore, stocks of wild fish are not adequate to meet the nutrient demands of the growing world population, and fish consumption choices also have a broad economic impact on the fishing industry. Most guidance does not account for ecological and economic impacts of different fish consumption choices. Conclusion: Despite the relative lack of information integrating the health, ecological, and economic impacts of different fish choices, clear and simple guidance is necessary to effect desired changes. Thus, more comprehensive advice can be developed to describe the multiple impacts of fish consumption. In addition, policy and fishery management inter-ventions will be necessary to ensure long-term availability of fish as an important source of human nutrition. PMID:22534056

  17. Fluorescence in situ hybridization on formalin-fixed and paraffin-embedded tissue

    DEFF Research Database (Denmark)

    Laub Petersen, Bodil; Zeuthen, Mette Christa; Pedersen, Sanni

    2004-01-01

    Fluorescence in situ hybridization (FISH) is widely used to study numerical and structural genetic abnormalities in both metaphase and interphase cells. The technique is based on the hybridization of labeled probes to complementary sequences in the DNA or RNA of the cells. Interphase FISH is most...... in time lapse between removal of tissue and fixation, duration of fixation, enzymatic pretreatment, hybridization conditions, and posthybridization washing conditions are important factors in the hybridization. In this study, we have listed the results of a systematic approach to improve FISH on isolated...

  18. Desain dan Uji Kinerja Fungsional Sistem Penggerak dan Kendali ROVERGARD

    Directory of Open Access Journals (Sweden)

    Moh Fikri Pomalingo

    2017-04-01

    Full Text Available Abstract The high population rate has an impact on increasing of land function change from agricultural land become housing and commercial building. As a result, it is difficult to get land for planting in the urban area. Therefore, this research is aimed to design equipment that can be used for planting in the narrow land using vertical gardening. This paper will report about design and functional testing of drive and control system on ROVERGARD. Drive system is based on water pump with additional gear train and chainsprocket mechanism. Control system use was on open loop type based on timer. The performance test of drive system was focused on electrical energy consumption and rotational speed of the system that was measured by multifunctional mini ammeter and tachometer. The control system was tested during 4 days, to evaluate their performances between set point and actual timing while filling water tank and rotate the system at maximal load condition. Electrical power consumption was 208 W at average rotational speed 2703 rpm. Increasing load caused an increase of energy consumption but made the drive rotation decline. The position control performance had on position error around 50 cm. Consequently, setting time on timer must be adjusted. Abstrak Laju pertumbuhan penduduk yang tinggi, mengakibatkan tingginya alih fungsi lahan pertanian menjadi bangunan perumahan dan komersial. Masalah ini mengakibatkan sulitnya mencari lahan pertanian di daerah padat penduduk khususnya perkotaan. Oleh karena itu perlu dirancang sebuah alat yang dapat digunakan untuk bercocok tanam di lahan sempit. Tujuan dari penelitian ini adalah mendesain dan menguji sistem penggerak dan kendali pada ROVERGARD. Sistem penggerak berasal dari pompa air yang dimodifikasi. Sedangkan sistem kendali menggunakan tipe open loop berbasis waktu dimana timer sebagai komponen utamanya. Pengujian kinerja penggerak difokuskan pada konsumsi listrik dan rpm yang diukur menggunakan

  19. Histamine fish poisoning revisited.

    Science.gov (United States)

    Lehane, L; Olley, J

    2000-06-30

    Histamine (or scombroid) fish poisoning (HFP) is reviewed in a risk-assessment framework in an attempt to arrive at an informed characterisation of risk. Histamine is the main toxin involved in HFP, but the disease is not uncomplicated histamine poisoning. Although it is generally associated with high levels of histamine (> or =50 mg/100 g) in bacterially contaminated fish of particular species, the pathogenesis of HFP has not been clearly elucidated. Various hypotheses have been put forward to explain why histamine consumed in spoiled fish is more toxic than pure histamine taken orally, but none has proved totally satisfactory. Urocanic acid, like histamine, an imidazole compound derived from histidine in spoiling fish, may be the "missing factor" in HFP. cis-Urocanic acid has recently been recognised as a mast cell degranulator, and endogenous histamine from mast cell degranulation may augment the exogenous histamine consumed in spoiled fish. HFP is a mild disease, but is important in relation to food safety and international trade. Consumers are becoming more demanding, and litigation following food poisoning incidents is becoming more common. Producers, distributors and restaurants are increasingly held liable for the quality of the products they handle and sell. Many countries have set guidelines for maximum permitted levels of histamine in fish. However, histamine concentrations within a spoiled fish are extremely variable, as is the threshold toxic dose. Until the identity, levels and potency of possible potentiators and/or mast-cell-degranulating factors are elucidated, it is difficult to establish regulatory limits for histamine in foods on the basis of potential health hazard. Histidine decarboxylating bacteria produce histamine from free histidine in spoiling fish. Although some are present in the normal microbial flora of live fish, most seem to be derived from post-catching contamination on board fishing vessels, at the processing plant or in the

  20. EVALUASI KUALITAS WARNA IKAN KLOWN Amphiprion percula Lacepède 1802 TANGKAPAN ALAM DAN HASIL BUDIDAYA

    Directory of Open Access Journals (Sweden)

    Sukarman Sukarman

    2018-01-01

    Full Text Available Kualitas warna ikan klown hasil budidaya lebih rendah dibandingkan tangkapan alam, hal ini dipengaruhi oleh berbagai faktor, namun belum ada data ilmiah sebagai dasar untuk melakukan perbaikan. Tujuan penelitian adalah menganalisis dan mengevaluasi kualitas warna ikan klown (Amphiprion percula hasil tangkapan alam dibandingkan dengan hasil budidaya. Kualitas warna diukur pada dua zona: zona-I kulit berwarna oranye antara insang dengan band warna putih pada tengah badan dan zona-II adalah bagian kulit warna oranye antara band putih tengah badan dengan band warna putih pada pangkal ekor, dengan parameter nilai L* (lightness, a* (redness, b* (yellowness, C (chroma, H (Hue. Analisis total karotenoid (TC dilakukan pada kulit kedua zona, sirip pektoral, sirip dorsal, sirip kaudal, dan serum darah. Analisis kromatografi lapis tipis (KLT dilakukan pada kulit dan sirip untuk mengonfirmasi jenis karotenoid dalam kulit dan sirip. Data kualitas warna dianalisis menggunakan t-test, hubungan kualitas warna dengan TC dianalisis dengan regresi sederhana, dan analisis deskriptif untuk hasil KLT. Hasil penelitian menunjukkan bahwa kualitas warna ikan klown tangkapan alam lebih baik dibanding budidaya, didukung oleh tingginya total karotenoid pada kulit zona-I, kulit zona-II, sirip pektoral, sirip dorsal, sirip kaudal, dan serum darah berturut-turut 51,64; 51,24; 136,40; 124,37; 194,18 mg/kg; dan 2,2 mg/mL; pada ikan hasil budidaya berurut-turut 2,5; 3,5; 8,45; 10,01; 23,43 mg/kg; dan 0,8 mg/mL. Hasil KLT menunjukkan bahwa jenis karotenoid pada kulit dan sirip ikan klown adalah astaxanthin, serta satu jenis karotenoid diduga zeaxanthin. Berdasarkan hasil penelitian, maka perlu ditambahkan pigmen karotenoid, dan prekursor pigmen lainnya melalui pakan untuk ikan klown budidaya. The color quality of cultured clownfish is not as good as the wild one. However, it’s influenced by several factors. However, but there is not enough scientific data to be used as the basis

  1. KONTEKS DAN KONSTRUKSI SOSIAL MENGENAI KEMATIAN ELEKTIF ( EUTHANASIA ∗

    Directory of Open Access Journals (Sweden)

    Helly Prajitno Soetjipto

    2015-09-01

    konteks sosial dan konstruksi sosial kematian. Euthanasia didiskusikan di dalam suatu kerangka pikir yang mencoba memberi perhatian kepada hal-hal yang kontekstual dan interpretatif fenomena sosial suatu proses kematian dan kejadian kematian

  2. Fluorescence fluctuation spectroscopy (FFS)

    CERN Document Server

    Tetin, Sergey

    2012-01-01

    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

  3. Fluorescent quantification of melanin.

    Science.gov (United States)

    Fernandes, Bruno; Matamá, Teresa; Guimarães, Diana; Gomes, Andreia; Cavaco-Paulo, Artur

    2016-11-01

    Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. PEMANFAATAN MINYAK BUAH MERAH, Pandanus conoideus Lam DAN CAROPHYLL PINK DALAM RANSUM PAKAN YUWANA IKAN KAKAP MERAH, Lutjanus sebae

    Directory of Open Access Journals (Sweden)

    Titiek Aslianti

    2016-11-01

    Full Text Available Ikan kakap merah, Lutjanus sebae termasuk komoditas budidaya yang banyak diminati konsumen. Kualitas benih untuk pembesaran selain ditentukan dari ukuran dan performansi fisik, juga berdasarkan warna ikan. Dalam penelitian ini telah dilakukan 4 perlakuan pakan yang diperkaya dengan Minyak Buah Merah (MBM dan Carophyll Pink (CP yaitu: (A 10 mL MBM/kg pakan; (B 10 mL MBM+1,4 g CP/kg pakan; (C 1,4 g CP/kg pakan, dan (D Kontrol. Hewan uji adalah yuwana kakap merah dengan rata-rata panjang dan bobot tubuh awal 11,32±0,76 cm dan 37,56±7,22 g. Ikan ditempatkan dalam bak fiber kapasitas 5 m3 dengan kepadatan 200 ekor/bak dan diberi pakan 2 kali sehari secara ad libitum serta dipelihara selama 2 bulan dengan pergantian air 200%-300%/hari. Kualitas yuwana diamati pada akhir penelitian melalui pengukuran panjang dan bobot tubuh, sedangkan performansi warna diamati dengan kamera digital dan dianalisis secara diskriptif menggunakan Microsoft Adobe Photoshop 8. Hasil penelitian menunjukkan bahwa MBM dan CP dapat dimanfaatkan dalam ransum pakan guna meningkatkan pertumbuhan dan performansi warna yuwana kakap merah. Pakan yang mengandung 10 mL MBM+1,4 g CP menghasilkan sintasan 94%, kenaikan pertumbuhan panjang dan bobot mutlak 58,92% dan 281%, konversi pakan 0,82 serta performansi yuwana berwarna merah cerah dengan nilai persentase rata-rata sebesar 89,58%±0,07%. Red emperor snapper, Lutjanus sebae is one of high demanded cultured fishes. The quality of seeds does not only depend on size and performance of fish, but also depends on fish color. A feeding experiment was conducted during two-months period by adding red fruit oil (RFO and carophyll pink (CP to every kilogram of pellet fed as the treatment i.e. (A 10 mL RFO/kg feed; (B 10 mL RFO+1.4 g CP/kg feed; (C 1.4 g CP /kg feed and (D Control. Two hundred seeds with initial total length of 11.32±0.76 cm and 37.56±7.22 g of body weight were stocked in a fiber glass tank of 5 m3. Feeding frequency was

  5. DIAGNOSIS DAN PENATALAKSANAAru LIMFOMA ORtsITA

    Directory of Open Access Journals (Sweden)

    Ardizal Rahman

    2014-12-01

    Full Text Available Limfoma orbita merujuk pada limfoma yang terjadi di konjungtiva, kelenjar lakrimal, palpebradan otot-otot ekstraokular. Limfoma primer non-Hodgkin (NHL dari orbita dapat ditemukanpada hanya 1o/o dari semua limforna non-Hodgkin. Anaiisis mutasi somatik pada regiovariabel (V dari immunoglobulin (ig dan segmen gen rantai berat (H telah menunjukkanperan dari stimulasi antigen kronik pada patogenesis limfoma /nucosa-associated lymphoidflssue (MALT. Patogen mikroba seperti Helicobacter pylaridan Chlamydia pneumonia dapaimendasari proses inflamasi dan pada akhirnya memicu akuisisi MALT juga memainkanperan penting dalam tranformasi maligna dan ekspansi klonal lanjutan limfoma. Penentuanstadium kanker sangat penting karena akan menentukan terapi apa yang akan diberikan dankemungkinan remisi dan prognosisnya. Berdasarkan sistem stadium Ann-Arbor, limfoma yangterbatas di orbita disebut sebagai stadium l, keterlibatan struktur sekitar (sinus paranasal,tonsil, dan hidung menjadikannya stadium ll. Stadium lll adalah penyakit nodal abdominaldibawah diafragma dan stadium lV merujuk pada keterlibatan yang tersebar dari satu ataulebih lokasi ekstranodal (hepar, sum-sum tulang atau sistem saraf pusat. Mayoritas pasiendatang dengan keluhan massa konjungtiva berwarna pink (91%, diikuti hiperemis konjungtiva(32%, propiosis (27%, massa palpebra atau orbita (19"fi, penurunan visus dan ptosis (6%,dan diplopia(2%. Bilateralitas terjadi pada 10% hingga 15% kasus dimana 80 % terjadisecarasimultan sedangkan 20% merupakan kondisi yang berurutan. Penilaian lanjut untuk stagingyang akurat dan perencanaan terapitermasuk anamnesis yang lengkap dan pemeriksaan fisik,pemeriksaan laboratorium rutin, elektroforesis protein sei-um, LDH serum, Fr-mikroglobulin,rontgen thoraks, CT scan thoraks, abdornen, dan pelvis, dan biopsisum-sum tulang. Diagnosapositif harus berdasarkan pada perneriksaan histologik dari sampeltumor yang memadai yangdiperoleh dengan biopsiorbita. Beberapa kriteria

  6. TRANSFORMASI ORGANISASIONAL DAN MSDM: HAMBATAN DAN IMPLIKASINYA PADA REKRUTMEN DAN SELEKSI

    Directory of Open Access Journals (Sweden)

    Licen Indahwati Darsono

    2002-01-01

    Full Text Available Rapid change of environment is an external force that causes the organization to transform. Fundamentally, the main purpose of the transformation is to change the organizational structure to be more flexible and competitive with fewer hierarchial levels, managers, and employees. The transformation needs to be radical, causing resistance from the organization's members. Their resistance can cause the transformation to fail, therefore organization must find ways to lessen this resistance. First, organization must learn about the diversity of its members' cultures and values. Second, organization must build its own organizational culture which can support the success of the transformation, by communicating it with the members of the organization. To support organizational culture building efforts, it needs changes in human resources practices, especially recruitment and selection. Abstract in Bahasa Indonesia : Perubahan yang cepat dalam lingkungan merupakan kekuatan eksternal yang mengakibatkan transformasi dalam sebuah organisasi. Pada dasarnya, tujuan utama dari transformasi tersebut adalah merubah struktur organisasi agar menjadi lebih fleksibel dan mampu bersaing, dengan tingkat structural yang sedikit, serta jumlah manajer dan karyawan yang lebih kecil. Transformasi tersebut harus menyeluruh, dan hal ini dapat menyebabkan resistensi dari para anggota organisasi yang memperhambat perubahan tersebut. Resistensi itu bisa menyebabkan perubahan tersebut batal, oleh karena itu organisasi harus mencari jalan untuk mengurangi hambatan-hambatan tersebut. Pertama, organisasi harus belajar keanekaragaman dari budaya dan nilai anggotanya. Kedua, organisasi harus mengembangkan budaya organisasi sendiri melalui komunikasi yang baik dengan anggotanya. Untuk mendukung usaha mengembangkan budaya organisasi, harus ada perubaban pada kebijakan sumber daya manusia, terutama dalam rekrutmen dan seleksi karyawan. Kata kunci: transformasi, resistensi, budaya

  7. Perbandingan Akurasi Pengukuran Suhu Dan Kelembaban Antara Sensor DHT11 Dan DHT22

    OpenAIRE

    Saptadi, Arief Hendra

    2014-01-01

    Suhu dan kelembaban merupakan dua objek pengukuran yang acapkali terdapat di dalam sistem akuisisi data. Terdapat banyak piranti sensor yang berfungsi untuk mengukur dua objek tersebut dan akurasi merupakan salah satu parameter yang dapat digunakan untuk memilihnya. DHT11 dan DHT22 adalah sensor seri DHT dari Aosong Electronics yang dapat melakukan pengukuran suhu dan kelembaban secara serempak dengan keluaran digital. Informasi tentang akurasi terdapat di dalam lembar data keduanya. Kendati ...

  8. Pertumbuhan dan Mutu Fisik Bibit Jabon (Anthocephalus Cadamba Miq.) di Polibag dan Politub

    OpenAIRE

    Junaedi, Ahmad

    2010-01-01

    Informasi pertumbuhan dan mutu fisik bibit jabon (Anthocephalus cadamba Miq.) di wadah bibit dengan volume yang berbeda diperlukan sebagai bagian yang akan diperhitungkan dalam pemilihan wadah bibit yang akan digunakan. Penelitian ini dilakukan untuk mengetahui pertumbuhan dan mutu fisik bibit jabon yang disapih pada wadah bibit polibag (volume 300 cm3) dan politub (volume 60 cm3). Tahapan penelitian meliputi : pembibitan serta pengamatan pertumbuhan dan penilaian mutu fisik bibit jabon yang ...

  9. Dampak belanja irigasi dan jaringan terhadap output PDRB sektor pertanian dan pertumbuhan ekonomi Kota Jambi

    OpenAIRE

    Aminah, Siti; Parmadi, Parmadi

    2018-01-01

    Penelitian ini bertujuan untuk menganalisis perkembangan Belanja irigasi dan jaringan serta perkembangan output GDP sektor pertanian Kota Jambi selama periode tahun 2005-2015 dan menganalisis pengaruh belanja irigasi dan jaringan terhadap  GDP Output sektor pertanian dan pertumbuhan ekonomi Kota Jambi. Hasil penelitian menunjukkan Selama periode penelitian tahun 2005-2015 dengan rata-rata pertumbuhan 61,98%, nilai rata-rata PDRB harga berlaku Kota Jambi sebesar 157.601,68 dalam juta rupi...

  10. PERFORMA PERTUMBUHAN, KOEFISIEN VARIASI, DAN HETEROSIS HASIL PERSILANGAN IKAN PATIN (Pangasius sp. PADA TAHAP PENDEDERAN II

    Directory of Open Access Journals (Sweden)

    Jadmiko Darmawan

    2017-05-01

    Full Text Available Hibridisasi merupakan salah satu teknik pemuliaan ikan dalam rangka mendapatkan varietas unggul sehingga mampu meningkatkan nilai produksi suatu komoditas ikan yang dibudidayakan. Tujuan penelitian ini adalah untuk mengevaluasi performa pertumbuhan, koefisien variasi, dan nilai heterosis dari hasil persilangan tiga spesies ikan patin sebagai tetua, yaitu patin siam (Pangasianodon hypophthalmus, patin jambal (Pangasius djambal, dan patin nasutus (Pangasius nasutus pada tahap pendederan II. Induk ikan patin siam dan patin jambal yang digunakan merupakan ikan yang sudah dirilis sebagai ikan budidaya, sedangkan induk ikan patin nasutus berasal dari perairan umum dan sedang dalam proses domestikasi sebagai ikan budidaya. Persilangan yang dibuat adalah: A f  patin jambal >0,05. Ikan patin SN memiliki performa yang lebih baik dari tetuanya dengan nilai heterosis bobot akhir, LPS bobot, panjang total, LPS panjang total, dan sintasan berturut-turut sebesar 110,87%; 19,78%; 36,14%, 36,09%; dan 15,04%; serta nilai koefisien variasi berkisar antara 0,00-11,08. Bobot akhir, panjang total dan sintasan ikan patin SJ juga lebih baik dari tetuanya dengan nilai heterosis berturut-turut sebesar 46,00%; 11,27%; dan 2,27%; namun untuk heterosis LPS bobot dan LPS panjang total bernilai negatif (-6,65% dan -1,01%, serta nilai koefisien variasi berkisar antara 0,00-12,75. Berdasarkan hasil penelitian tersebut menunjukkan bahwa ikan patin hibrida SN berpotensi sebagai ikan budidaya dalam rangka peningkatan produksi ikan patin daging putih selain dari ikan patin hibrida  SJ (pasupati yang telah dirilis ke masyarakat. Hybridization is a fish breeding technique in order to obtain high yielding varieties as to increase the production value of a farmed fish. The purpose of this study was to evaluate the growth performance, the coefficient of variation and heterosis value of the result of crossing three species of catfish as a parent, which was striped catfish

  11. Fluorescent nanodiamond for biomedicine

    International Nuclear Information System (INIS)

    Milos Nesladek

    2014-01-01

    NV centers in diamond have gained strong interest as a novel tool for quantum information processing, quantum computing and quantum photonics. These applications are based on fluorescent and spin properties of NV-centres. However, in some conditions NV- can lose an electron and turn to NV0. The occupation of NV0 and NV- charge states depend on the position of their ground states with respect to the Fermi level and the mechanism of the charge transfer. Interestingly, that the charge switch has important implications on applications of fluorescent nanodiamond (fND) to nano-biology and nano-medicine. fND can be used for bio-marking and bio-tracking but also for the monitoring of targeted delivery to the cells. In this presentation we review the current state-of-the art for using fND particles for fluorescent bio imaging in cells and discuss the charge transfer and its luminescence stability by using ultra high sensitive spectroscopy methods to study the NV0 and NV- state occupation. (author)

  12. Quantitative and qualitative determination of enrofloxacin residues in fish tissues

    OpenAIRE

    Đorđević Vesna; Baltić M.; Ćirković M.; Kilibarda Nataša; Glamočlija Nataša; Stefanović S.; Miščević Mirjana

    2009-01-01

    Presence of enrofloxacin residues in fish liver, kidney and muscle tissue was investigated after per os application of the drug. For the purpose of determination of enrofloxacin, the following analytical methods were used: microbiological method - plate pH 8 with Escherichia coli ATCC 11303 and HPLC method with fluorescence detection. After a 5-day oral treatment of carps, enrofloxacin residues in tissues were determined up to the 10th day after the end of the drug application. Enrofloxacin c...

  13. SIS - Fish Assessment

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Fish Assessment data set within the Species Information System (SIS) constraints information related to fishery stock assessments, including assessment meta-data...

  14. West Coast Fishing Ethnography

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Created as part of a 2012 BOEM study on OCS renewable energy space-use conflicts, this data contains the commercial and recreational fishing locations off the...

  15. In Place of Fishing

    DEFF Research Database (Denmark)

    Ounanian, Kristen

    Communities historically reliant on fisheries have faced changing circumstances in terms of their livelihoods, identities, demographics, and viabilities. I examine various manifestations of fisheries dependence and the process of transition as related to six cases of fishing communities undergoing...

  16. Fish-friendly future

    International Nuclear Information System (INIS)

    Brookshier, P.; Newman, Gemma

    2001-01-01

    The latest US research into ways of reducing the harm to fish from hydroelectric turbines is outlined. Laboratory studies, field studies and advanced computational studies are being carried out to improve the understanding of and reduce the effects of stress and injury to fish from turbines. The Advanced Hydro Turbine System programme is part of the Department of Energy's Hydropower Programme. Turbine passage injuries are caused by a number of mechanisms, leaving the fish either dead or stunned. Advanced turbine technology can help to minimise fish injury and can add dissolved oxygen to the discharged water, thus improving water quality. Turbine modifications are aimed at all species, but studies have focussed on salmon, trout and eels. The new minimum gap runner (MGR) appear as efficient as standard Kaplan turbines

  17. Freshwater Fish Communities

    Science.gov (United States)

    Freshwater fish are ecologically important in stream ecosystems, and they provide people with significant food, recreation, and conservation value as biological indicator of freshwater streams. Historically, the streams and rivers of southern New England supported moderately dive...

  18. Fishing Community Profiles

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — To enable fisheries managers to comply with National Standard 8 (NS8), NMFS social scientists around the nation are preparing fishing community profiles that present...

  19. Wacana Penjarahan Dan Kekerasan Simbolik Terhadap Petani

    Directory of Open Access Journals (Sweden)

    Agus Sudibyo

    2002-01-01

    Full Text Available Perlawanan kaum petani sudah lama terjadi dalam konteks sejarah hubungan antara si kaya dan si miskin dan antara kaum petani dan negara. Persoralannya selalu bahwa negara, aparat keamanan dan pemilik modal telah mengambil alih secara paksa surplus ekonomi dari petani. Mereka bekerja sama dalam aksi penyerobotan tanah petani, pendirian perkebunan-perkebunan dan dalam menghadapi gerakan protes petani. Negara bukan hanya menghadapi gerakan perlawanan petani dengan represi-represi fisik. Secara sistematis negara juga melakukan propaganda dengan menciptakan gambaran-gambaran yang unfavourable tentang petani pelaku penjarahan dalam berbagai representasi wacana. Dalam batas tertentu, media massa justru memperkuat kecenderungan ini dengan menonjolkan dimensi kekerasan, pemberontakan dan anarkisme dalam mewacanakan aksi-aksi penjarahan petani.

  20. Motivasi, Kebiasaan, dan Keamanan Penggunaan Internet

    Directory of Open Access Journals (Sweden)

    Zinggara Hidayat

    2017-01-01

    Full Text Available Abstract: This study investigates motivation, habits, and security of internet use by high schools and universities students in Jakarta, Bogor, Depok, Tangerang, and Bekasi. This descriptive and quantitative reasearch uses survey method. The findings of the study show that the strongest motivation is to show self existence, entertainment, and academic purposes. At some points, the habit of using the internet also makes the students face some risks such as verbal and nonverbal abuses, bullying, pornoghrapy, account hacking, and the risk of interaction with unknown parties. Abstrak: Penelitian ini menyelidiki motivasi, kebiasaan, dan keamanan penggunaan internet di antara siswa sekolah menengah atas dan universitas di Jakarta, Bogor, Depok, Tangerang, dan Bekasi. Penelitian deskriptif kuantitatif ini menggunakan metode survei. Hasil penelitian menunjukkan bahwa motivasi paling kuat adalah untuk memperlihatkan eksistensi diri, pencarian hiburan, dan penyelesaian tugas akademis. Kebiasaan penggunaan internet oleh pelajar dan mahasiswa menghadapi beberapa risiko, seperti kekerasan verbal dan nonverbal,

  1. SOSIAL MEDIA DAN PERUBAHAN INDEKS PRESTASI MAHASISWA

    Directory of Open Access Journals (Sweden)

    Risnah Risnah

    2015-12-01

    Tujuan dari penelitian ini adalah mengetahui jumlah mahasiswa semester II, IV, dan VI Jurusan Keperawatan yang menggunakan internet secara aktif dan karak-teristiknya, membandingkan hasil belajar mahasiswa semester II-III, IV-V, dan VI-VII Jurusan Keperawatan, dan mengetahui pengaruh sosial media (facebook dan twitter terhadap perubahan indeks prestasi mahasiswa, dengan jumlah res-ponden sebanyak 150 orang. Penelitian ini adalah deskriptif dengan desain pene-litian kausal. Pengumpulan data dengan menggunakan instrumen berupa kuesio-ner, dengan jumlah responden sebanyak 150 orang. Adapun hasil penelitian me-nunjukkan bahwa indeks prestasi mahasiswa dengan penggunaan sosial media umumnya responden menyatakan sosial media tidak mempengaruhi indeks prestasi. Dari hasil analisis uji statistik diperoleh terdapat pengaruh antara sosial media (facebook dan twitter dengan indeks prestasi. Dengan begitu, penggunaan sosial media harus digunakan sebagaimana mestinya, agar mahasiswa/i yang ak-tif pengunaan sosial media tidak mempengaruhi indeks prestasinya.

  2. PERANAN SEKTOR PERIKANAN DALAM PEMBANGUNAN WILAYAH DAN STRATEGI PENGEMBANGANYA DALAM RANGKA OTONOMI DAERAH KABUPATEN BALANGAN

    Directory of Open Access Journals (Sweden)

    Syahrida Ariani

    2016-06-01

    , Perdagangan dan Koperasi memfasilitasi peta jalur pemasaran bagi petani ikan sehingga harga ikan bisa tetap stabil, 9 Perlu melakukan pembinaan dan pendampingan kepada masyarakat terutama untuk diversifikasi poduk menjadi produk olahan, 10 Perbaikan fasilitas infrastruktur dan fungsi sarana dan prasarana produksi budidaya perikanan melalui perbaikan saluran irigasi dan BBI, 11 Pemerintah daerah lebih mensosialisasikan skim perkreditan untuk rakyat KUR melalui sosialisasi kepada masyarakat. Balangan Regency has a fairly large waters resources and potentially increase revenues original areas, expected role of Government that has the authority Balangan Regency as Executive exploration and wealth management waters should be able to dig into the potential of the fishery to be more optimal in order to exercise the autonomy of the region.  In General, this research aims to apply the techniques of planning regional development Balangan Regency. Whereas in particular this research aims to know the role of the fisheries sector to the economy and setting the Balangan Regency alternative strategies for the development of fisheries sector in the framework of regional autonomy in Balangan Regency. Research methods used are descriptive qualitative, and used case studies approaches and analysis of the data used  descriptive analysis, analysis of profit and SWOT analysis (Strategic Issues and problem identification. Based on the results of the SWOT analysis and the calculation of weighting matrix with QSP obtained the alternative order of precedence for the development strategy of the fishery as follows: 1 local government commitment to the development of the fisheries sector through the allocation of the budget, 2 of local government through the Office of PTPHPP improves  the role of BBI and UPR to produce quality seeds and stem and have a certification, 3 increases the role and function of fisheries extension, officers, 4 increase the utilization potential of land fish breeding with

  3. Persepsi Santri dan Kiai terhadap Pluralisme Agama di Pendidikan Ulama Tarjih Muhammadiyah (PUTM dan Aswaja Nusantara Yogyakarta

    Directory of Open Access Journals (Sweden)

    Zainal Arifin

    2017-05-01

    Abstrak: Perdebatan pluralisme agama masih mengemuka dalam konteks masyarakat majemuk di Nusantara. Bahkan, tidak jarang menimbulkan konflik dan perang pemikiran. Menarik melihat perspektif kiai dan santri Muhammadiyah dan NU terhadap isu pluralisme melalui praktik pendidikan di Ulama Tarjih Muhammadiyah (PUTM Yogyakarta dan Pondok Pesantren Aswaja Nusantara Yogyakarta yang dianggap representatif karena membawa muatan konsep, ide dan paradigma dari Muhammadiyah dan NU. Penelitian ini bersifat kualitatif,  data diperoleh dari wawancara dan studi kepustakaan dengan saling memberikan verifikasi, koreksi dan pelengkap. Hasil penelitian ini, PUTM dan Pesantren Aswaja Nusantara memiliki konsep yang sama dalam memegang teguh akidah, yakni sikap eksklusif dan menolak pluralisme secara teologis namun menerimanya secara sosiologis. Sehingga, paradigma dari dua lembaga tersebut merepresentasikan dari paradigma Muhammadiyah dan NU dalam merespon fenomena pluralisme agama dan budaya.

  4. VAKSIN DENGUE DAN PERKEMBANGANNYA SAAT INI DAN DI MASA MENDATANG

    Directory of Open Access Journals (Sweden)

    Triwibowo Ambar Garjito

    2012-09-01

    Full Text Available Dengue virus merupakan salah satu virus anggota dari famili Flaviviridae yang sejak tahun 1956 telah dikenal dapat menimbulkan demam dengue maupun demam berdarah dengue (DBD. Penyakit yang ditularkan oleh nyamuk Aedes aegypti ini diperkirakan telah menjangkiti pada selatar 50-100 juta manusia dengan 500.000 kasus di antaranya dalam manifestasi yang ganas yang dikenal sebagai dengue haemorrhagic fever dan dengue shock syndrome dan 25.000 di antaranya berakibat fatal (meninggal. Saat ini pengembangan vaksin merupakan salah satu solusi yang diharapkan dapat menekan penyebaran penyakit tersebut. E (envelope merupakan salah satu bagian dari protein struktural virus yang sangat penting dalam pengembangan vaksin, yaitu sebagai badan yang memproduksi antibodi netralisasi untuk protein. Non-struktural protein l juga telah diketahui sebagai salah satu komponen penting dalam pengembangan vaksin oleh karena kemampuannya untuk dapat diekspresi pada permukaan sel yang diinfeksi yang dapat menjadi target untuk immune cytolisis. Ada dua pendekatan yang digunakan dalam memproduksi suatu vaksin dengue, yaitu: a. Vaksin hidup yang telah dilemahkan (live attenuated vaccine: b. Vaksin hasil rekayasa (engineered vaccine. Penelitian terhadap vaksin DENV baik rekombinan maupun non-rekombinan yang didasarkan pada uji virus telah dilakukan secara terus-menerus baik pada monyet dan manusia. Sampai saat ini telah dikembangkan sejumlah kandidat vaksin DENV yang berdasar pada tetravalent virus dengue, yaitu a. vaksin konvensional, b. vaksin dengue rekombinan berdasar pada flavivirus, c. vaksin intertypic chimeric, d. vaksin chimerivac, e. vaksin dengue rekombinan menggunakan vector non-ftavivirus dan f. vector adenovirus. Namun demikian, sampai sekarang belum ada vaksin yang siap digunakan untuk menangkal infeksi ke empat serotype virus dengue, sehingga masih diharapkan untuk pengembangan virus lebih lanjut.   Kata kunci: Aedes aegypti, dengue virus, vaksin dengue.

  5. BAHAYA LATEN INFEKSI GONORE DAN KLAMIDIA DI ASIA SELATAN & ASIA TENGGARA, DI INDONESIA DAN KOTA MANADO

    Directory of Open Access Journals (Sweden)

    Aron Pirade

    2014-04-01

    menempati urutan tertinggi. Wilayah Asia Selatan dan Asia Timur-Selatan (juga disebut Asia Tenggara adalah wilayah dengan prevalensi Gonore dan Klamidia terbesar. Prevalensi Gonore di tahun 1995 dan 1999 yaitu 29.11 dan 27.2 juta penduduk wilayah Asia Selatan dan Asia Tenggara. Klamidia, di tahun 1995 dan tahun 1999 yaitu 40.48 dan 42.89 juta penduduk wilayah Asia Selatan dan Asia Tenggara. Di Indonesia sendiri, pada 11 area penelitian menunjukkan bahwa prevalensi Gonore dalam wilayah Indonesia menunjukkan rata-rata prosentasenya 20%-40% dari keseluruhan STI pada wanita beresiko tinggi terkena STI. Sedangkan infeksi oleh Klamidia prosentasenya 15%- 45% dari keseluruhan STI pada wanita beresiko tinggi terkena penyakit kelamin. Prosentase Gonore dan Klamidia di Kota Manado rata-rata 15-30%. Tingginya prevalensi infeksi Gonore dan Klamidia di Asia Tenggara, ternyata diikuti juga dengan tingginya perkembangan infeksi Gonore dan Klamidia di Indonesia dan Kota Manado.

  6. Social learning in fish

    OpenAIRE

    Atton, Nicola

    2010-01-01

    Social learning is known to be a common phenomenon in fish, which they utilise under many different contexts, including foraging, mate-choice and migration. Here I review the literature on social learning in fish and present two studies. The first examines the ability of threespined sticklebacks to use social learning in the enhancement of food preferences. The second study examines the ability of both threespined sticklebacks and ninespined sticklebacks to use social learning in the avoidanc...

  7. CARD-FISH for Environmental Microorganisms: Technical Advancement and Future Applications

    OpenAIRE

    Kubota, Kengo

    2012-01-01

    Fluorescence in situ hybridization (FISH) has become a standard technique in environmental microbiology. More than 20 years have passed since this technique was first described, and it is currently used for the detection of ribosomal RNA, messenger RNA, and functional genes encoded on chromosomes. This review focuses on the advancement and applications of FISH combined with catalyzed reporter deposition (CARD, also known as tyramide signal amplification or TSA), in the detection of environmen...

  8. Mercury speciation with fluorescent gold nanocluster as a probe.

    Science.gov (United States)

    Yang, Jian-Yu; Yang, Ting; Wang, Xiao-Yan; Chen, Ming-Li; Yu, Yong-Liang; Wang, Jian-Hua

    2018-05-11

    Fluorescent nanoparticles are widely used for sensing biologically significant species. However, it is rarely reported for the discrimination or speciation of metal species. In this work, we report for the first time the speciation of mercury (Hg 2+ ) and methylmercury (CH 3 Hg + ) by taking advantage of the fluorescence feature of folic acid-capped gold nanoclusters (FA-AuNCs). FA-Au NCs exhibit an average size of 2.08±0.15 nm and a maximum emission at λ ex /λ em = 280/440 nm with a quantum yield of 27.3%. It is interesting that Hg 2+ causes a significant quench on the fluorescence of FA-Au NCs, whereas CH 3 Hg + leads to a remarkable fluorescence enhancement. Based on this discriminative fluorescent response between Hg 2+ and CH 3 Hg + , a novel nanosensor for the speciation of CH 3 Hg + and Hg 2+ was developed, providing limits of detection (LOD) of 28 nM for Hg 2+ and 25 nM for CH 3 Hg + within 100-1000 nM. This sensing system is highly selective to mercury. Its practical applications were further demonstrated by the analysis of CH 3 Hg + and the speciation of mercury (CH 3 Hg + and Hg 2+ ) in environmental water and fish samples.

  9. NCEP ATP III dan Framingham score

    OpenAIRE

    Hasan, Refli; Fahila, Reny

    2016-01-01

    Laporan ini merupakan Program Pendidikan Kolesterol National yang diperbaharui yaitu pedoman klinis untuk melakukan pengujian kolesterol dan manajemen. ATP III dibuat berdasarkan bukti dan laporan ekstensif yang akan menjadi referensi dan rekomendasi ilmiah. Laporan ATP III dapat dijadikan pedoman untuk pemberian terapi penurun kolesterol yang intensif dalam praktek. Pedoman ini hanya sebagai informasi , tidak dapat mempengaruhi secara mutlak dalam penilaian klinis dokter yang akhirnya menent...

  10. DESAIN, TATA LETAK, DAN KONSTRUKSI TAMBAK

    Directory of Open Access Journals (Sweden)

    Akhmad Mustafa

    2008-12-01

    Full Text Available Komoditas yang umum dibudidayakan di tambak Indonesia adalah udang dan ikan bandeng yang menjadi komoditas unggulan untuk dikembangkan. Salah satu faktor penting yang sangat menentukan keberhasilan usaha budidaya tambak adalah rekayasa tambak yang mencakup disain, tata letak, dan konstruksi tambak. Secara umum, desain petakan tambak merupakan perencanaan bentuk tambak yang meliputi: ukuran panjang dan lebar petakan, kedalaman, ukuran pematang, ukuran berm, dan ukuran saluran keliling, serta ukuran dan letak pintu air. Tata letak suatu unit tambak harus memenuhi tujuan seperti: menjamin kelancaran mobilitas operasional sehari-hari, menjamin kelancaran dan keamanan pasok air, serta pembuangannya, dapat menekan biaya konstruksi tanpa mengurangi fungsi teknis dari unit tambak yang dibangun dan mempertahankan kelestarian lingkungan. Konstruksi tambak yang menggambarkan proses pengerjaan tambak harus disesuaikan dengan desain dan tata letak yang telah ada. Rekayasa tambak diarahkan pada kemampuan untuk menciptakan kondisi yang sesuai dengan keadaan alami yang dituntut oleh organisme akuatik yang dibudidayakan sehingga produktivitas tambak meningkat, efisien secara ekonomis, dan berkelanjutan.

  11. KONTEKTUALISASI DAN (KEMUNGKINAN KONSEKUENSINYA BAGI PSIKOLOGI

    Directory of Open Access Journals (Sweden)

    Prof. Supratiknya

    2015-09-01

    jaran di perguruan tinggi seperti kita kenal sekarang (selanjutnya disebut psikologi, lahir di Jerman pada penghujung abad ke‐19. Selanjutnya psikologi berkembang pesat di Amerika Utara khususnya Amerika Serikat dan negara‐negara lain di Eropa yang lebih dulu mengalami industrialisasi khususnya di Eropa Barat. 1 Alhasil, paling tidak sampai dasawarsa 1980‐an perkembangan dan persebaran psikologi di tingkat global ditandai oleh ketimpangan di antara apa yang oleh Moghaddam (1987 disebut tiga dunia tempat psikologi dikembangkan dan dipraktekkan, disimak antara lain berdasarkan besarnya produksi pengetahuan psikologis yang disebarluaskan melalui penyelenggaraan pendidikan tinggi psikologi maupun lewat publikasi ilmiah berupa baik berkala maupun buku rujukan dan buku teks.

  12. Innovation in utilization of fish tanks for fish culture among fish ...

    African Journals Online (AJOL)

    This study investigated innovation in utilization of fish tanks for fish culture among fish farmers in Obio/Akpor Local Government Area of Rivers State, Nigeria. Data for this study was obtained through the administration of questionnaire and scheduled interview to 120 sampled fish farmers randomly selected from the study ...

  13. Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridisation

    NARCIS (Netherlands)

    Lim, K.B.; Wennekes, J.; Jong, de J.H.S.G.M.; Jacobsen, E.; Tuyl, van J.M.

    2001-01-01

    Detailed karyotypes of Lilium longiflorum and L. rubellum were constructed on the basis of chromosome arm lengths, C-banding, AgNO3 staining, and PI-DAPI banding, together with fluorescence in situ hybridisation (FISH) with the 5S and 45S rDNA sequences as probes. The C-banding patterns that were

  14. Fluorescence in situ hybridization for phytoplasma and endophytic bacteria localization in plant tissues.

    Science.gov (United States)

    Bulgari, Daniela; Casati, Paola; Faoro, Franco

    2011-11-01

    In the present study, we developed a rapid and efficient fluorescence in situ hybridization assay (FISH) in non-embedded tissues of the model plant Catharanthus roseus for co-localizing phytoplasmas and endophytic bacteria, opening new perspectives for studying the interaction between these microorganisms. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. STATUS POPULASI IKAN NAPOLEON DI WILAYAH TAMAN NASIONAL BUNAKEN DAN KABUPATEN KARAS FAK-FAK

    Directory of Open Access Journals (Sweden)

    Isa Nagib Edrus

    2016-03-01

    Full Text Available Penelitian ikan napoleon di perairan Bunaken pada Oktober 2012 dan Kabupaten Karas pada Nopember 2010 bertujuan untuk mengetahui status populasi ikan tersebut di bawah usaha perlindungan otoritas Taman Nasional Bunaken dan pasca penutupan penangkapannya di Karas.  Metode Underwater Visual Census digunakan untuk mendapatkan data jumlah individu dan ukuran tubuh ikan napoleon.  Hasil penelitian menunjukkan bahwa rata-rata kepadatan ikan napoleon dari luas wilayah sensus 19,26 hektar di Bunaken adalah 1,71 individu per hektar dan dari luas sensus 44,61 hektar di perairan Karas adalah 1,41 individu per hektar. Distribusi panjang frekuensi populasi ikan ini di kedua wilayah tersebut menunjukkan adanya dua kelompok umur, dimana interval ukuran panjang adalah antara 15 cm sampai 100 cm.  Perkembangan populasi ikan tersebut di Bunaken selama 7 tahun menunjukkan peningkatan sebesar 427,5%, sedang di Karas selama kurun waktu 5 tahun sebesar 298%. Namun pertumbuhan populasi sebesar ini ternyata belum masuk pada kategori status kelimpahan yang membaik. Jadi perlindungan ikan ini disarankan untuk lebih diperketat dengan cara melakukan moratorium.   Study on humphead wrasse fish in Bunaken and Karas district waters recpectively in October 2012 and November 2010 were aimed to determine population status of the fish under protecting regulations of the Bunaken National Park Authority and fishing closed session in Karas.  A method used to get population sizes and body sizes was the Napoleon Underwater Visual Census. The results showed that an average of fish density was 1,71 individual/ha of 19.26 ha in area census at Bunaken and it was 1,41 individual/ha of 44.61ha in area census of Karas waters. Distribution frequencies of the fish population were under two cohorts  with body size interval ranged from 15cm to 100 cm. Population trend during seven  years in Bunaken have increased in 427.5 % and those during five years in Karas have increase in 298

  16. Clinical application of fluorescence in situ hybridization for prenatal diagnosis

    Directory of Open Access Journals (Sweden)

    Shu-fang JIANG

    2012-07-01

    Full Text Available Objective To establish and optimize the procedures of fluorescence in situ hybridization(FISH), and evaluate its clinical value in rapid prenatal diagnosis of fetal numerical abnormality of chromosomes 21, 18, 13, X, Y. Methods Amniotic fluid or fetal blood was sampled by routine invasive procedures. After the amniotic fluid cells or fetal blood cells were separated and sequentially processed with hypotonic solution, fixation solution, smear and high temperature, they were hybridized in situ with two panels of specific fluorescence probes to detect numerical abnormality of chromosomes 21, 18, 13, X, Y. All the samples were also cultured and analyzed for their karyotype by conventional methods. Results When it was used as a diagnostic criterion of chromosomal number that the fluorescence signals were observed in ≥90% cells, GLP 13/GLP 21 probe panel showed 2 green/2 red fluorescence signals and CSP18/CSP X/CSP Y probe panel showed 2 blue/2 yellow (female or 2 blue/1 yellow/1 red fluorescence signals (male under normal condition. The test reports of all 196 cases were sent out in 72-96 hours, and 7 cases of Down syndrome, 2 cases of trisomy 18 and 1 case of sex chromosomal numerical abnormality were detected, which were accordant with karyotype analysis results reported one month later. Conclusions FISH has potential for clinical application, and is applicable to rapid prenatal diagnosis of fetal numerical abnormality of chromosomes 21, 18, 13, X, Y. The rapid FISH, together with conventional karyotyping, offer a valuable means for prenatal diagnosis of fetal aneuploidies.

  17. TASAWUF DAN TANTANGAN PERUBAHAN SOSIAL

    Directory of Open Access Journals (Sweden)

    Yusno Abdullah Otta

    2010-12-01

    Full Text Available Abstract: Sufism in the eras of globalization and information often faces considerable challenges. The function and role of religion in modern life have in some aspects diminished, including sufism because it is claimed to have played no or less role in resolving problems nor stimulating social change. As one of Islamic traditions, sufism makes ihsan as its essential teaching. By this concept, sufism encourages humans to pray to God wholeheartedly. Sufism is now required to show its own existence and function by making fundamental contribution for modern life, an era which differ socio-culturally from the one when sufism firstly emerged. Sufism thus needs to adapt itself to the changes in the era of information and globalization so that it will remain relevant and suitable for human progress. Sufism needs to release itself from its exclusivism and to avoid being trapped in its own logic, namely speculative-trancendentalism Abstrak: Tasawuf di era globalisasi dan informasi seperti saat ini tengah menghadapi tantangan yang sangat berat. Kehidupan modern sudah sampai pada taraf tidak lagi mempercayai fungsi dan peran agama, termasuk di dalamnya tasawuf, sebagai ‘sesuatu’ yang dapat memberikan solusi bagi perubahan sosial. Sebagai manifestasi paripurna dari ajaran Islam, tasawuf berintikan pada konsep Ihsān yang mengajarkan untuk beribadah kepada Sang Pencipta secara ikhlas. Untuk menunjukkan eksistensinya, tasawuf dituntut peran aktifnya secara konstruktif-solutif terhadap berbagai kebutuhan manusia modern yang amat berbeda dengan setting maupun struktur masyarakat pada saat tasawuf “dilahirkan” pada zamannya. Tasawuf dituntut untuk memerlukan “baju” baru di era informasi yang kerap berubah ini, agar tetap realible dan suitable dengan kemajuan zaman. Hal itu dapat dilakukan dengan berusaha “keluar” dari eksklusifismenya dengan meminjam tradisi filsafat analitik pasca positivisme dan tradisi eksistensialis serta dinamika filsafat

  18. Sensitive determination of nucleic acids using organic nanoparticle fluorescence probes

    Science.gov (United States)

    Zhou, Yunyou; Bian, Guirong; Wang, Leyu; Dong, Ling; Wang, Lun; Kan, Jian

    2005-06-01

    This paper describes the preparation of organic nanoparticles by reprecipitation method under sonication and vigorous stirring. Transmission electron microscopy (TEM) was used to characterize the size and size distribution of the luminescent nanoparticles. Their average diameter was about 25 nm with a size variation of ±18%. The fluorescence decay lifetime of the nanoparticles also was determined on a self-equipped fluorospectrometer with laser light source. The lifetime (˜0.09 μs) of nanoparticles is about three times long as that of the monomer. The nanoparticles were in abundant of hydrophilic groups, which increased their miscibility in aqueous solution. These organic nanoparticles have high photochemical stability, excellent resistance to chemical degradation and photodegradation, and a good fluorescence quantum yield (25%). The fluorescence can be efficiently quenched by nucleic acids. Based on the fluorescence quenching of nanoparticles, a fluorescence quenching method was developed for determination of microamounts of nucleic acids by using the nanoparticles as a new fluorescent probe. Under optimal conditions, maximum fluorescence quenching is produced, with maximum excitation and emission wavelengths of 345 and 402 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range 0.4-19.0 μg ml -1 for calf thymus DNA (ct-DNA) and 0.3-19.0 μg ml -1 for fish sperm DNA (fs-DNA). The corresponding detection limits are 0.25 μg ml -1 for ct-DNA and 0.17 μg ml -1 for fs-DNA. The relative standard deviation of six replicate measurements is 1.3-2.1%. The method is simple, rapid and sensitive with wide linear range. The recovery and relative standard deviation are very satisfactory.

  19. Fishing down the largest coral reef fish species.

    Science.gov (United States)

    Fenner, Douglas

    2014-07-15

    Studies on remote, uninhabited, near-pristine reefs have revealed surprisingly large populations of large reef fish. Locations such as the northwestern Hawaiian Islands, northern Marianas Islands, Line Islands, U.S. remote Pacific Islands, Cocos-Keeling Atoll and Chagos archipelago have much higher reef fish biomass than islands and reefs near people. Much of the high biomass of most remote reef fish communities lies in the largest species, such as sharks, bumphead parrots, giant trevally, and humphead wrasse. Some, such as sharks and giant trevally, are apex predators, but others such as bumphead parrots and humphead wrasse, are not. At many locations, decreases in large reef fish species have been attributed to fishing. Fishing is well known to remove the largest fish first, and a quantitative measure of vulnerability to fishing indicates that large reef fish species are much more vulnerable to fishing than small fish. The removal of large reef fish by fishing parallels the extinction of terrestrial megafauna by early humans. However large reef fish have great value for various ecological roles and for reef tourism. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Analisis Kinerja EIGRP dan OSPF pada Topologi Ring dan Mesh

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2014-01-01

    Full Text Available ABSTRAK EIGRP (Enhanced Interior Gateway Routing Protocol dan OSPF (Open Shortest Path Fisrt adalah routing protokol yang banyak digunakan pada suatu jaringan komputer. EIGRP hanya dapat digunakan pada perangkat Merk CISCO, sedangkan OSPF dapat digunakan pada semua merk jaringan. Pada penelitian ini dibandingkan delay dan rute dari kedua routing protokol yang diimplementasikan pada topologi Ring dan Mesh. Cisco Packet Tracer 5.3 digunakan untuk mensimulasikan kedua routing protokol ini. Skenario pertama adalah perancangan jaringan kemudian dilakukan pengujian waktu delay 100 kali dalam 5 kasus. Skenario kedua dilakukan pengujian trace route untuk mengetahui jalur yang dilewati paket data lalu memutus link utama. Pada skenario kedua juga dilakukan perbandingan nilai metric dan cost hasil simulasi dengan perhitungan rumus. Skenario ketiga dilakukan pengujian waktu konvergensi untuk setiap routing protokol pada setiap topologi. Hasilnya EIGRP lebih cepat 386 µs daripada OSPF untuk topologi Ring sedangkan OSPF lebih cepat 453 µs daripada EIGRP untuk topologi Mesh. Hasil trace route menunjukan rute yang dipilih oleh routing protokol yaitu nilai metric dan cost yang terkecil. Waktu konvergensi rata-rata topologi Ring pada EIGRP sebesar 12,75 detik dan 34,5 detik pada OSPF sedangkan topologi Mesh di EIGRP sebesar 13 detik dan 35,25 detik di OSPF. Kata Kunci: EIGRP, OSPF, Packet Tracer 5.3, Ring, Mesh, Konvergensi ABSTRACT EIGRP (Enhanced Interior Gateway Routing Protocol and OSPF (Open Shortest Path Fisrt is the routing protocol that is widely used in a computer network. EIGRP can only be used on devices Brand CISCO, while OSPF can be used on all brands of network. In this study comparison of both the delay and the routing protocol implemented on Ring and Mesh topology. Cisco Packet Tracer 5.3 is used to simulate both the routing protocol. The first scenario is the design of the network and then do the test of time delay 100 times in 5 cases. The

  1. Analisis Kinerja EIGRP dan OSPF pada Topologi Ring dan Mesh

    Directory of Open Access Journals (Sweden)

    DWI ARYANTA

    2016-02-01

    Full Text Available ABSTRAK EIGRP (Enhanced Interior Gateway Routing Protocol dan OSPF (Open Shortest Path Fisrt adalah routing protokol yang banyak digunakan pada suatu jaringan komputer. EIGRP hanya dapat digunakan pada perangkat Merk CISCO, sedangkan OSPF dapat digunakan pada semua merk jaringan. Pada penelitian ini dibandingkan delay dan rute dari kedua routing protokol yang diimplementasikan pada topologi Ring dan Mesh. Cisco Packet Tracer 5.3 digunakan untuk mensimulasikan kedua routing protokol ini. Skenario pertama adalah perancangan jaringan kemudian dilakukan pengujian waktu delay 100 kali dalam 5 kasus. Skenario kedua dilakukan pengujian trace route untuk mengetahui jalur yang dilewati paket data lalu memutus link utama. Pada skenario kedua juga dilakukan perbandingan nilai metric dan cost hasil simulasi dengan perhitungan rumus. Skenario ketiga dilakukan pengujian waktu konvergensi untuk setiap routing protokol pada setiap topologi. Hasilnya EIGRP lebih cepat 386 µs daripada OSPF untuk topologi Ring sedangkan OSPF lebih cepat 453 µs daripada EIGRP untuk topologi Mesh. Hasil trace route menunjukan rute yang dipilih oleh routing protokol yaitu nilai metric dan cost yang terkecil. Waktu konvergensi rata-rata topologi Ring pada EIGRP sebesar 12,75 detik dan 34,5 detik pada OSPF sedangkan topologi Mesh di EIGRP sebesar 13 detik dan 35,25 detik di OSPF. Kata Kunci : EIGRP, OSPF, Packet Tracer 5.3, Ring, Mesh, Konvergensi ABSTRACT EIGRP (Enhanced Interior Gateway Routing Protocol and OSPF (Open Shortest Path Fisrt is the routing protocol that is widely used in a computer network. EIGRP can only be used on devices Brand CISCO, while OSPF can be used on all brands of network. In this study comparison of both the delay and the routing protocol implemented on Ring and Mesh topology. Cisco Packet Tracer 5.3 is used to simulate both the routing protocol. The first scenario is the design of the network and then do the test of time delay 100 times in 5 cases. The

  2. RNA detection in situ with FISH-STICs.

    Science.gov (United States)

    Sinnamon, John R; Czaplinski, Kevin

    2014-02-01

    The ability to detect RNA molecules in situ has long had important applications for molecular biological studies. Enzyme or dye-labeled antisense in vitro runoff transcripts and synthetic oligodeoxynucleotides (ODN) both have a proven track record of success, but each of these also has scientific and practical drawbacks and limitations to its use. We devised a means to use commercially synthesized oligonucleotides as RNA-FISH probes without further modification and show that such probes work well for detection of RNA in cultured cells. This approach can bind a high concentration of fluorescent ODN to a short stretch of an RNA using commercial DNA synthesis outlets available to any laboratory. We call this approach for creating in situ hybridization probes Fluorescence In Situ Hybridization with Sequential Tethered and Intertwined ODN Complexes (FISH-STICs). We demonstrate that one FISH-STIC probe can detect mRNA molecules in culture, and that probe detection can be improved by the addition of multiple probes that can be easily adapted for robust mRNA quantification. Using FISH-STICs, we demonstrate a nonoverlapping distribution for β-actin and γ-actin mRNA in cultured fibroblasts, and the detection of neuron-specific transcripts within cultured primary hippocampal neurons.

  3. KEJAHATAN PORNOGRAFI Upaya Pencegahan dan Penanggulangannya di Kabupaten Ponorogo

    Directory of Open Access Journals (Sweden)

    Irma Rumtianing Uswatul Hanifah

    2013-12-01

    Full Text Available Pemerintah telah mengeluarkan undang-undang No 44 Tahun 2008 tentang pornografi. Tujuan dari undang-undang tersebut salah satunya mewujudkan dan memelihara tatanan kehidupan masyarakat yang beretika, berkepribadian luhur, menjungjung tinggi nilai-nilai Ketuhanan Yang Maha Esa, serta menghormati harkat dan martabat kemanusiaan. Dalam perkembangannya, materi pornografi mengalami pertumbuhan dan penyebaran yang pesat seiring dengan perkembangan dan kemajuan teknologi informasi dan komunikasi. Peredaran dan penyebaran film porno kini semakin pesat karena ditopang dengan kecanggihan sarana informasi dan komunikasi salah satunya media internet yang bisa diakses oleh masyarakat pada setiap saat. Tulisan berikut akan mengkaji bagaimana upaya dan trategi yang dilakukan oleh Pemerintah Kabupaten Ponorogo dalam mencegah dan menanggulangi kejahatan pornografi di Kabupaten Ponorogo, apa hambatan dan bagaimana solusi dalam pencegahan serta penanggulangan kejahatan pornografi di Kabupaten Ponorogo serta bagaimana formulasi kebijakan untuk pencegahan dan penanggulangan kejahatan pornografi di Kabupaten Ponorogo. Kata kunci: Pornografi, Polres, KP3A

  4. PERANAN LEMAK PAKAN DALAM MENDUKUNG PERKEMBANGAN EMBRIO, DERAJAT PENETASAN TELUR, DAN SINTASAN LARVA IKAN BAUNG (Mystus nemurus

    Directory of Open Access Journals (Sweden)

    Ningrum Suhenda

    2016-11-01

    Full Text Available Penelitian ini dilakukan di Balai Riset Perikanan Budidaya Air Tawar (BRPBAT, Bogor dengan tujuan untuk mengetahui pengaruh kadar lemak pakan yang berbeda terhadap perkembangan embrio, derajat pembuahan, penetasan telur, dan sintasan larva ikan baung. Pakan yang digunakan berupa pakan buatan yang mengandung lemak dengan kadar yang berbeda yaitu 4%, 6%, 8%, 10%, dan 12% dengan sumber lemak yang dipergunakan adalah campuran minyak ikan dan minyak nabati dengan perbandingan 1:1. Bobot rata-rata induk ikan baung pada awal percobaan 420,89±72,10 g/ekor dan dipelihara di dalam kolam beton. Rancangan percobaan yang digunakan yaitu rancangan acak lengkap dengan 5 perlakuan dan 3 ulangan. Parameter yang diamati yaitu derajat penetasan, perkembangan embrio, dan sintasan larva. Hasil penelitian menunjukkan bahwa lemak pakan merupakan faktor penting yang erat hubungannya dengan perkembangan embrio yang terlihat mulai dari pembuahan sampai dengan penetasan yaitu berkisar antara 25 jam 5 menit sampai 26 jam 37 menit. Stadia morula, blastula, dan gastrula terjadi relatif lebih cepat pada induk yang diberi pakan dengan kadar lemak 8%, akan tetapi induk yang diberi pakan dengan kadar lemak 6%, telur yang dihasilkan lebih cepat menetas. Perlakuan kadar lemak 6% dan 8% memperoleh derajat pembuahan di atas 98%, derajat penetasan telur di atas 95%, dan sintasan larva di atas 99%. Selanjutnya pakan ini cukup untuk mendukung terjadinya proses perkembangan embrio secara sempurna dan mendukung sintasan larva. Fish feed should contain sufficient amount of lipid and fatty acid because these elements play eminent roles in supporting reproduction and survival rate of larvae. Fatty acid in egg mass has an important role in fish breeding technology development because it influences the early development of embryogenesis and development of embryo. The research was conducted at the Research Institute for Freshwater Aquaculture, Bogor aimed to study the effect of different

  5. Fluorescence in situ hybridization with reference to biodosimetry: a review

    International Nuclear Information System (INIS)

    Venkatachalam, P.; Paul, S.F.D.; Jeevanram, R.K.

    1996-01-01

    Many advances have taken place in the field of radiation biodosimetry in the recent past. Measurement of dicentric chromosome aberrations, was first developed and followed by micronuclei scoring. These, however, are unstable type aberrations and the cells carrying such aberrations are eliminated from the body in few years. They are therefore of use primarily in case of accidental exposures. The challenge is to measure the cumulative radiation exposure resulting from normal operations by measuring stable chromosome aberrations. Banding technique can measure stable chromosome aberration but require long time to analyse the banding pattern to study translocations. On the other hand fluorescence in situ hybridization (FISH) technique is sensitive, fast and easy to identify the translocations as the chromosomes involved in translocation are painted with different colours. This review brings out the requirements of various materials, their preparations, method of detection of fluorescence etc. for carrying out FISH. The experience of various laboratories using FISH in the monitoring of radiation absorbed dose is discussed. (author)

  6. KANDUNGAN ASAM AMINO, TAURIN, MINERAL MAKRO-MIKRO, DAN VITAMIN B12 UBUR-UBUR (Aurelia aurita SEGAR DAN KERING

    Directory of Open Access Journals (Sweden)

    - - Nurjanah

    2014-06-01

    Full Text Available Tujuan penelitian adalah menentukan komposisi gizi, asam amino, taurin, mineral makro dan mikro,dan vitamin B12 pada ubur-ubur (Aurelia aurita segar dan kering. Asam amino esensial pada ubur-uburyaitu arginina, leusina, valina, treonina, lisina, isoleusina, fenilalanina, metionina, dan histidina, sedangkanasam amino non esensial yaitu asam glutamat, glisina, asam aspartat, serina, alanina, dan tirosina. Asamamino esensial tertinggi segar dan kering adalah arginina sebesar 1,72% (bk dan 1,44% (bk dan terendahhistidina yaitu sebesar 0,19% (bk dan 0,13% (bk. Asam amino non esensial segar dan kering tertinggiadalah asam glutamat dan glisina yaitu sebesar 3,26% (bk dan 2,62% (bk dan terkecil tirosina sebesar0,38% (bk dan 0,41% (bk. Taurin segar sebesar 2,68% (bk dan kering sebesar 0,67% (bk. Mineral makrotertinggi segar dan kering adalah natrium yaitu 180.092,1 ppm (bk dan 111.209,4 ppm (bk, terkecil adalahkalsium yaitu 5.750,2 ppm (bk dan 11,1 ppm (bk. Mineral mikro tertinggi segar dan kering adalah iodium yaitu8.291,5 ppm (bk dan 1.800 ppm (bk dan yang terkecil adalah tembaga yaitu 1,1 ppm (bk dan 0,6 ppm (bk.Vitamin B12 segar adalah 396,6 μm/100 g (bk dan kering 63,5 μm/100 g (bk.Kata kunci: asam amino, mineral, taurin, ubur-ubur (Aurelia aurita, vitamin B12

  7. Fluorescent microthermographic imaging

    Energy Technology Data Exchange (ETDEWEB)

    Barton, D.L.

    1993-09-01

    In the early days of microelectronics, design rules and feature sizes were large enough that sub-micron spatial resolution was not needed. Infrared or IR thermal techniques were available that calculated the object`s temperature from infrared emission. There is a fundamental spatial resolution limitation dependent on the wavelengths of light being used in the image formation process. As the integrated circuit feature sizes began to shrink toward the one micron level, the limitations imposed on IR thermal systems became more pronounced. Something else was needed to overcome this limitation. Liquid crystals have been used with great success, but they lack the temperature measurement capabilities of other techniques. The fluorescent microthermographic imaging technique (FMI) was developed to meet this need. This technique offers better than 0.01{degrees}C temperature resolution and is diffraction limited to 0.3 {mu}m spatial resolution. While the temperature resolution is comparable to that available on IR systems, the spatial resolution is much better. The FMI technique provides better spatial resolution by using a temperature dependent fluorescent film that emits light at 612 nm instead of the 1.5 {mu}m to 12 {mu}m range used by IR techniques. This tutorial starts with a review of blackbody radiation physics, the process by which all heated objects emit radiation to their surroundings, in order to understand the sources of information that are available to characterize an object`s surface temperature. The processes used in infrared thermal imaging are then detailed to point out the limitations of the technique but also to contrast it with the FMI process. The FMI technique is then described in detail, starting with the fluorescent film physics and ending with a series of examples of past applications of FMI.

  8. RNA Imaging with Multiplexed Error Robust Fluorescence in situ Hybridization

    Science.gov (United States)

    Moffitt, Jeffrey R.; Zhuang, Xiaowei

    2016-01-01

    Quantitative measurements of both the copy number and spatial distribution of large fractions of the transcriptome in single-cells could revolutionize our understanding of a variety of cellular and tissue behaviors in both healthy and diseased states. Single-molecule Fluorescence In Situ Hybridization (smFISH)—an approach where individual RNAs are labeled with fluorescent probes and imaged in their native cellular and tissue context—provides both the copy number and spatial context of RNAs but has been limited in the number of RNA species that can be measured simultaneously. Here we describe Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH), a massively parallelized form of smFISH that can image and identify hundreds to thousands of different RNA species simultaneously with high accuracy in individual cells in their native spatial context. We provide detailed protocols on all aspects of MERFISH, including probe design, data collection, and data analysis to allow interested laboratories to perform MERFISH measurements themselves. PMID:27241748

  9. Kemampuan Penalaran Dan Komunikasi Matematis : Apa, Mengapa, Dan Bagaimana Ditingkatkan Pada Mahasiswa

    Directory of Open Access Journals (Sweden)

    Cita Dwi Rosita

    2014-01-01

    Full Text Available Matematika diberikan kepada semua siswa tanpa terkecuali agar terlatih berpikir secara logis, analitis, sistematis, dan kreatif. Dengan kompetensi-kompetensi tersebut diharapkan siswa dapat memiliki kemampuan menerima, mengelola, dan memanfaatkan pengetahuan yang diperolehnya untuk bertahan hidup dalam keadaan yang selalu berubah dan kompetitif. Latihan berpikir, merumuskan dan memecahkan masalah serta mengambil kesimpulan akan membantu siswa untuk mengembangkan pemikirannya atau intelegensinya. Dengan demikian, semakin banyak siswa berlatih memecahkan masalah matematis maka akan semakin mengerti dan berkembang cara berpikirnya. Kemahiran siswa dalam memecahkan masalah matematis, dipengaruhi oleh kemampuannya dalam memahami matematika. Kemampuan bernalar berperan penting dalam memahami matematika. Bernalar secara matematis merupakan suatu kebiasaan berpikir, dan layaknya suatu kebiasaan, maka penalaran semestinya menjadi bagian yang konsisten dalam setiap pengalaman-pengalaman matematis siswa. Dari pengalaman-pengalaman awal siswa belajar materi matematika, penting bagi guru untuk membantu siswa memahami bahwa penegasan-penegasan harus selalu mempunyai alasan. Komunikasi matematis berperan penting pada proses pemecahan masalah. Melalui komunikasi ide bisa menjadi objek yang dihasilkan dari sebuah refleksi, penghalusan, diskusi, dan pengembangan. Proses komunikasi juga membantu dalam proses pembangunan makna dan pempublikasian ide. Ketika para siswa ditantang untuk berpikir dan bernalar tentang matematika dan mengomunikasikan hasil pikiran mereka secara lisan atau dalam bentuk tulisan, sebenarnya mereka sedang belajar menjelaskan dan meyakinkan. Mendengarkan penjelasan lain, berarti sedang memberi kesempatan kepada siswa untuk mengembangkan pemahaman mereka.

  10. KEADILAN ORGANISASIONAL DAN KONSEKUENSINYA TERHADAP ORGANIZATIONAL CITIZENSHIP BEHAVIOR GURU SMA DAN SMK KOTA MADIUN*

    Directory of Open Access Journals (Sweden)

    Veronika Agustini Srimulyani

    2017-11-01

    Full Text Available Keadilan organisasional memiliki potensi untuk menciptakan manfaat besar bagi organisasi dan karyawan, dan salah satu manfaatnya adalah menumbuhkan extra-role behavior (ERB atau organizational citizenship behavior (OCB. Tujuan dari penelitian ini untuk menganalisis konsekuensi keadilan organisasional (keadilan distributif, keadilan prosedural, dan keadilan interaksional pada organizational citizenship behavior guru SMA dan guru SMK di Kota Madiun. OCB dibagi menjadi dua jenis yaitu citizenship behaviors directed toward individuals (OCB-I dan citizenship behaviors directed towards the organisation (OCB-O. Penelitian ini menggunakan pendekatan kuantitatif dengan metode survei. Sampel penelitian ini adalah 409 guru, yang dipilih secara non-acak. Data diperoleh dengan menyebarkan kuesioner. Analisis data menggunakan analisis regresi. Hasil penelitian disimpulkan: keadilan distributif dan keadilan interaksional berpengaruh positif signifikan terhadap OCB-I dan OCB-O; keadilan prosedural tidak berpengaruh signifikan terhadap OCB-I tetapi keadilan prosedural berpengaruh positif signifikan terhadap OCB-O.

  11. Cowlitz Falls fish passage

    International Nuclear Information System (INIS)

    1995-09-01

    The upper Cowlitz was once home to native salmon and steelhead. But the combined impacts of overharvest, farming, logging and road building hammered fish runs. And in the 1960s, a pair of hydroelectric dams blocked the migration path of ocean-returning and ocean-going fish. The lower Cowlitz still supports hatchery runs of chinook, coho and steelhead. But some 200 river miles in the upper river basin--much of it prime spawning and rearing habitat--have been virtually cut off from the ocean for over 26 years. Now the idea is to trap-and-haul salmon and steelhead both ways and bypass previously impassable obstacles in the path of anadromous fish. The plan can be summarized, for the sake of explanation, in three steps: (1) trap and haul adult fish--collect ocean-returning adult fish at the lowermost Cowlitz dam, and truck them upstream; (2) reseed--release the ripe adults above the uppermost dam, and let them spawn naturally, at the same time, supplement these runs with hatchery born fry that are reared and imprinted in ponds and net pens in the watershed; (3) trap and haul smolts--collection the new generation of young fish as they arrive at the uppermost Cowlitz dam, truck them past the three dams, and release them to continue their downstream migration to the sea. The critical part of any fish-collection system is the method of fish attraction. Scientists have to find the best combination of attraction system and screens that will guide young fish to the right spot, away from the turbine intakes. In the spring of 1994 a test was made of a prototype system of baffles and slots on the upriver face of the Cowlitz Falls Dam. The prototype worked at 90% efficiency in early tests, and it worked without the kind of expensive screening devices that have been installed on other dams. Now that the success of the attraction system has been verified, Harza engineers and consultants will design and build the appropriate collection part of the system

  12. Fish robotics and hydrodynamics

    Science.gov (United States)

    Lauder, George

    2010-11-01

    Studying the fluid dynamics of locomotion in freely-swimming fishes is challenging due to difficulties in controlling fish behavior. To provide better control over fish-like propulsive systems we have constructed a variety of fish-like robotic test platforms that range from highly biomimetic models of fins, to simple physical models of body movements during aquatic locomotion. First, we have constructed a series of biorobotic models of fish pectoral fins with 5 fin rays that allow detailed study of fin motion, forces, and fluid dynamics associated with fin-based locomotion. We find that by tuning fin ray stiffness and the imposed motion program we can produce thrust both on the fin outstroke and instroke. Second, we are using a robotic flapping foil system to study the self-propulsion of flexible plastic foils of varying stiffness, length, and trailing edge shape as a means of investigating the fluid dynamic effect of simple changes in the properties of undulating bodies moving through water. We find unexpected non-linear stiffness-dependent effects of changing foil length on self-propelled speed, and as well as significant effects of trailing edge shape on foil swimming speed.

  13. Immunity to fish rhabdoviruses

    Science.gov (United States)

    Purcell, Maureen K.; Laing, Kerry J.; Winton, James R.

    2012-01-01

    Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabdoviruses, but several studies also indicate a role for cell-mediated immunity. Survival of acute rhabdoviral infection is also dependent on innate immunity, particularly the interferon (IFN) system that is rapidly induced in response to infection. Paradoxically, rhabdoviruses are sensitive to the effects of IFN but virulent rhabdoviruses can continue to replicate owing to the abilities of the matrix (M) protein to mediate host-cell shutoff and the non-virion (NV) protein to subvert programmed cell death and suppress functional IFN. While many basic features of the fish immune response to rhabdovirus infections are becoming better understood, much less is known about how factors in the environment affect the ecology of rhabdovirus infections in natural populations of aquatic animals.

  14. Freshwater and fish

    International Nuclear Information System (INIS)

    Saxen, R.

    1997-01-01

    Severe radioactive contamination of the freshwater environment could have serious consequences for both drinking water and fish. Most of the Nordic countries have an abundance of freshwater lakes and rivers. Finland alone has about 56,000 lakes, each with a surface area of 1 hectare or more. Nearly 10% of Finland's surface is covered with lakes and rivers. In Sweden, about 9% of the surface area is freshwater, in Norway about 5%, and in Denmark only about 2%. Freshwater plays a minor role in Iceland, but even there numerous rivers discharge from the volcanic soils to the Ocean. Cs-137 and 90 Sr are likely to be the most important radionuclides with respect to long term radioactive contamination of freshwater. If radioactive deposition occurs in the absence of snow and ice radionuclides will contaminate the surface water directly and may rapidly enter the aquatic food chain. Fish which eat contaminated plankton become contaminated almost immediately. Deposition during summer increases the transfer for radionuclides to fish since fish metabolism is faster during the warm season. During the cold period, fish metabolism is slow and thus uptake and excretion of radiocaesium are also slow. (EG)

  15. Immunity to fish rhabdoviruses.

    Science.gov (United States)

    Purcell, Maureen K; Laing, Kerry J; Winton, James R

    2012-01-01

    Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabdoviruses, but several studies also indicate a role for cell-mediated immunity. Survival of acute rhabdoviral infection is also dependent on innate immunity, particularly the interferon (IFN) system that is rapidly induced in response to infection. Paradoxically, rhabdoviruses are sensitive to the effects of IFN but virulent rhabdoviruses can continue to replicate owing to the abilities of the matrix (M) protein to mediate host-cell shutoff and the non‑virion (NV) protein to subvert programmed cell death and suppress functional IFN. While many basic features of the fish immune response to rhabdovirus infections are becoming better understood, much less is known about how factors in the environment affect the ecology of rhabdovirus infections in natural populations of aquatic animals.

  16. Immunity to Fish Rhabdoviruses

    Directory of Open Access Journals (Sweden)

    Maureen K. Purcell

    2012-01-01

    Full Text Available Members of the family Rhabdoviridae are single-stranded RNA viruses and globally important pathogens of wild and cultured fish and thus relatively well studied in their respective hosts or other model systems. Here, we review the protective immune mechanisms that fish mount in response to rhabdovirus infections. Teleost fish possess the principal components of innate and adaptive immunity found in other vertebrates. Neutralizing antibodies are critical for long-term protection from fish rhabdoviruses, but several studies also indicate a role for cell-mediated immunity. Survival of acute rhabdoviral infection is also dependent on innate immunity, particularly the interferon (IFN system that is rapidly induced in response to infection. Paradoxically, rhabdoviruses are sensitive to the effects of IFN but virulent rhabdoviruses can continue to replicate owing to the abilities of the matrix (M protein to mediate host-cell shutoff and the non‑virion (NV protein to subvert programmed cell death and suppress functional IFN. While many basic features of the fish immune response to rhabdovirus infections are becoming better understood, much less is known about how factors in the environment affect the ecology of rhabdovirus infections in natural populations of aquatic animals.

  17. A fluorescence scanning electron microscope

    International Nuclear Information System (INIS)

    Kanemaru, Takaaki; Hirata, Kazuho; Takasu, Shin-ichi; Isobe, Shin-ichiro; Mizuki, Keiji; Mataka, Shuntaro; Nakamura, Kei-ichiro

    2009-01-01

    Fluorescence techniques are widely used in biological research to examine molecular localization, while electron microscopy can provide unique ultrastructural information. To date, correlative images from both fluorescence and electron microscopy have been obtained separately using two different instruments, i.e. a fluorescence microscope (FM) and an electron microscope (EM). In the current study, a scanning electron microscope (SEM) (JEOL JXA8600 M) was combined with a fluorescence digital camera microscope unit and this hybrid instrument was named a fluorescence SEM (FL-SEM). In the labeling of FL-SEM samples, both Fluolid, which is an organic EL dye, and Alexa Fluor, were employed. We successfully demonstrated that the FL-SEM is a simple and practical tool for correlative fluorescence and electron microscopy.

  18. PEMANFAATAN SOSIAL MEDIA DALAM PENYULUHAN PERTANIAN DAN PERIKANAN DI INDONESIA

    Directory of Open Access Journals (Sweden)

    Kadhung Prayoga

    2017-04-01

    Full Text Available ABSTRAKPertukaran informasi menjadi masalah yang mendapat sorotan dalam kegiatan penyuluhan pertanian dan perikanan. Sulitnya petani mengakses informasi ini kemudian memunculkan solusi dengan memanfaatkan teknologi informasi seperti media sosial. Sehingga, paper ini bertujuan untuk mengetahui bagaimana pelaksanaan penyuluhan yang memanfaatkan media sosial ini lewat sebuah studi literature terhadap sumber data sekunder. Dari hasil analisis penggunaan facebook dirasa masih sangat kurang optimal karena tidak ada update informasi terkait kegiatan perikanan. Namun, Kementerian Pertanian justru sangat aktif dalam menggunakan facebook. Sedangkan untuk pemanfaatan twitter, keduanya sama-sama aktif untuk berinteraksi dengan masyarakat. Pemanfaatan video conference dinilai sangat baik untuk Pusat Penyuluhan dan Pemberdayaan Masyarakat Kelautan dan Perikanan, namun masih kurang dioptimalkan oleh Kementerian Pertanian. Secara rutin dua institusi ini memperbaharui informasi terkait kegiatan pertanian dan perikanan seperti: budi daya, teknologi, maupun pemasaran. Sehingga, dapat disimpulkan bahwa kegiatan penyuluhan yang memanfaatkan media sosial harus terus dioptimalkan karena jumlah penggunanya yang terus meningkat.UTILIZATION OF SOCIAL MEDIA IN AGRICULTURE AND FISHERIES EXTENSION ACTIVITY IN INDONESIAABSTRACTThe exchange of information becomes a problem that gets the spotlight in the agriculture and fisheries extension activities. The difficulty for farmers to access this information is then led to solutions that take advantage of information technology such as social media.Thus, this paper aims to determine how the implementation of social media outreach utilizing it through a literature study on secondary data sources. From the analysis of the use of facebook it is still a very sub-optimal because there is no update information related to fishing activities. However, the Ministry of Agriculture is very much active in using facebook. As for the use of

  19. ANALISIS TINGKAT KEPUASAN DAN HARAPAN PASEN TERHADAP PELAYANAN PUSKESMAS DAN RUMAH SAKIT DAERAH PROVINSI DIY

    Directory of Open Access Journals (Sweden)

    Sunarto Sunarto

    2010-10-01

    Full Text Available Kepuasan merupakan perasaan seseorang mengenai kesenangan atau kekecewaan sebagai hasil membandingkan antara kinerja dan harapan. Jumlah kunjungan Puskesmas tahun 2007 sebesar 3.094.027 pasien yang terdiri dari 3.076442 rawat jalan dan 17.585 rawat inap. Penelitian untuk mengentahui gambaran tingkat kepuasan dan untuk mengetahui perbedaan tingkat kepuasan dan harapan pasien yang mendapatkan pelayanan Puskemas dan rumah sakit menurut wilayah Kabupatet / Kota di Propinsi DIY Penelitian ini merupakan penelitian deskriptif analitik dengan menggunakan rancangan cros sectional. Instrumen penelitian yang digunakan adalah kuisioner dengan 35 item pernyataan yang diambil dari atribut-atribut SERVQUAL dalam 5 dimensi. Total sampel untuk lima Kabupaten Kota sebanyak 1000, masing-masing Kabupaten Kota 200. Penelitian ini dilakukan terhadap pasien yang menerima pelayanan kesehatan di Puskesmas dan Rumah Sakit Daerah di Kabupaten-Kota Daerah Istimewa Yogyakarta. Penilaian tingkat kepuasan pasien ketika mereka berobat ke Puskesmas dan Rumah Sakit di Propinsi DIY, dengan menggunakan skala O sampai 4. Hasilnya adalah dimensi tangibles, rata-rata kepuasan pasien (2,96 cukup dan harapan pasien (3,38 pada dimensi reliability, rata-rata kepuasan pasien (3,01 bagus dan harapan pasien (3,40. Pada dimensi responsiveness, rata-rata kepuasan pa-sien (3,07 bagus dan harapan pasien (3,42. Dimensi assurance, rata-rata kepuasan pasien (3, 1 0 bagus dan harapan pasien (3 ,42. pada dimensi emphaty, rata-rata kepuasan pasien (3,02 bagus dan harapan pasien (3,35. Hasil uji statistik didapatkan nilai p > 0.05, berarti pada alpha 5%, dapat disimpulkan bahwa tidak terdapat perbedaan tingkat kepuasan diantara kelima wilayah kabupaten/kota di DIY. Penilaian tingkat kepuasan pasien, ketika mereka berkunjung ke Puskesmas dan Rumah Sakit di DIY secara rata-rata bagus dan tidak ada perbedaan tingkat kepuasan menurut wilayah Kabupaten-Kota di Propinsi DIY. Kata Kunci : Kepuasan, harapan

  20. KEMUNCULAN DAN TINGKAH LAKU PESUT (Orcaella brevirostris (Owen in Gray 1866 SEBAGAIMAMALIATERANCAMLANGKA DI PERAIRANKUBU RAYADANKAYONGUTARAKALIMANTAN BARAT

    Directory of Open Access Journals (Sweden)

    Regi Fiji Anggawangsa

    2015-12-01

    2013. Selama pengamatan hanya diketahui terdapat satu kelompok pesut sebanyak 4-6 individu yang terdiri dari pesut muda dan dewasa. Kelompokan itu dijumpai di muara Sungai Bumbun pada kedalamanan perairan 11 meter. Tingkah laku yang teramati menunjukkan gerombolan pesut umumnya memburu kelompokan ikan dan sesekali menyemburkan air dari blowhole nya. Berdasarkan ciri-ciri morfologi dan tingkah laku yang teramati serta kondisi lingkungan perairan setempat maka perairan di Kabupaten Kubu Raya dan Kabupaten Kayong Utara yang terletak di Provinsi Kalimantan Barat diduga merupakan perairan yang potensial sebagai habitat pesut (Orcaella brevirostris. Irrawaddy dolphins (Orcaella brevirostris (Owen in Gray 1866 is one of aquatic mammals species who populations are increasingly threatened. The lack information about population of Irrawaddy dolphins in West Kalimantan waters makes the conservation of this species and its management were still not optimized yet. This research aims to identify the characteristics and behavior of dolphins found in Kubu Raya and Kayong Utara waters of West Kalimantan. The observation was held on April 2013, exploring coastal waters and estuaries which are expected to be the habitats of Irrawaddy dolphins using binoculars. There are 4-6 individuals of Irrawaddy dolphin found at the mouth of Bumbun River. A group consist of young and adults dolphins at the depth of 11 meters. The observed behavior is chasing schooling fish and occasionally spitting water from his blowhole. Based on morphological character and fish behavior observed, and environmental condition parameters, indicated that the water surrounding of Kubu Raya and Kayong Utara District were potential habitat for Irrawaddy dolphins.

  1. Development of a fluorescent cryocooler

    International Nuclear Information System (INIS)

    Edwards, B.C.; Buchwald, M.I.; Epstein, R.I.; Gosnell, T.R.; Mungan, C.E.

    1995-01-01

    Recent work at Los Alamos National Laboratory has demonstrated the physical principles for a new type of solid-state cryocooler based on anti-Stokes fluorescence. Design studies indicate that a vibration-free, low-mass ''fluorescent cryocooler'' could operate for years with efficiencies and cooling powers comparable to current commercial systems. This paper presents concepts for a fluorescent cryocooler, design considerations and expected performance

  2. Produksi Panel Dinding Bangunan Tahan Gempa dan Ramah Lingkungan dari Limbah Bahan Berbahaya dan Beracun Industri Minyak dan Gas

    Directory of Open Access Journals (Sweden)

    Luqman Hakim

    2015-10-01

    Full Text Available Berdasarkan hasil uji karakteristik fisik terhadap panel dinding dari komposit limbah industry migas berupa activated alumina, sandblasting dan glasswall yang telah dilakukan pada tahun pertama diketahui bahwa kuat lentur tertinggi diperoleh dari sampel B4 yaitu sebesar 67,8 Kg/Cm2 dengan standar DIN 1101 17 Kg/cm2, kuat desak sampel B 2 68,31 N/mm2 dengan standar bata merah 25 N/mm2 dan batako 20 N/mm2 dan tingkat keausan terendah diperoleh dari sampel 37 streap. Dari hasil tersebut diketahui bahwa uji telah memiliki kemampuan lebih tinggi jika dibandingkan dengan standar yang berlaku. Maka pada penelitian lanjutan yang akan dilakukan bertujuan untuk mempelajari apakah produk panel dinding ini ramah lingkungan sehingga aman bagi kesehatan manusia dan lingkungan sekitarnya.Penelitian dilakukan dengan menggunakan metode uji toxicity characteristic leaching procedure (TCLP dan LC50 terhadap produk panel dinding terbaik. Uji TCLP yang akan dilakukan yaitu dengan cara mendestruksi dan ekstraksi produk panel dinding dengan menggunakan rotating agitator selama 24 jam kemudian diuji dengan menggunakan AAS untuk mengetahui konsentrasi logam berat yang terdapat dalam produl panel dinding. Adapun untuk uji LC50 dilakukan dengan menggunakan hewan uji larva udang atau tikus.Berdasarkan hasil uji TCLP dan LC50 diketahui bahwa: a Kadar kandungan logam berat yang terdapat di dalam wall panel setelah dilakukan uji TCLP ternyata berada dibawah baku mutu seperti yang telah ditetapkan dalam PP No.85 Tahun 1999. Jadi ini artinya produk wall panel dalam penelitian ini ramah lingkungan, b pengujian terhadap bahan baku wall panel, Limbah Activated Alumina, Sandblasting dan Glasswoll sebelum di solidifikasi dapat mematikan sebesar 50% hewan uji pada konsentrasi 116.667 ppm dalam waktu 96 jam, dan c hasil uji LC50 terhadap produk wall panel selama 96 jam tidak menunjukkan adanya kematian hewan uji. Dari hasil penelitian ini dapat disimpulkan bahwa produk wall panel dari

  3. UPAYA MENINGKATKAN HASIL BELAJAR, AKTIVITAS DAN SIKAP PADA MATERI GETARAN, GELOMBANG DAN BUNYI, MELALUI METODE DISKUSI, OBSERVASI, DAN EKSPERIMEN

    Directory of Open Access Journals (Sweden)

    Yuni Lestari Purnomowati

    2016-03-01

    Full Text Available Penelitian dilakukan menggunakan metodePenelitian Tindaan Kelas (PTK. Penelitian ini dilaksanakan di  SMKN 3 Metro pada siswa Kelas XI-TKJa semester genapTahun Pelajaran 2012/2013 berjumlah 31 siswa.Sasaran perubahan dalam penelitian ini yaitu peningkatan hasil, aktivitas dan sikap siswa terhadap pelajaran Fisika. Peneliti dapat menyimpulkan bahwa peningkatan hasil belajargetaran, gelombang dan bunyi terjadi karena kerjasama siswa selama proses pembelajaran menumbuhkan suasana yang nyaman sekaligus kompetitif. Perubahan sikap siswa terhadap pelajaran Fisika semakin baik. Ketika pembelajaran fisika mampu memberikan apa yang diharapkan oleh siswa, yaitu siswa merasa nyaman, tidak terintimidasi oleh guru dan teman karena keterbatasan yang dimilikinya, terlibat dengan teman sejawat pada saat menyelesaikan tugas-tugas yang diberikan dengan diskusi, melakukan observasi, dan bereksperimen, maka persepsi terhadap fisika sebagai objek menjadi berubah lebih baik. Adanya interaksi siswa dengan media pembelajaran karena kegiatan mendiskusikan, mengobservasi, dan melakukan eksperimen mendorong siswa menjadi aktif selama proses pembelajaran. Penelitian dilakukan menggunakan metodePenelitian Tindaan Kelas (PTK. Penelitian ini dilaksanakan di  SMKN 3 Metro pada siswa Kelas XI-TKJa semester genapTahun Pelajaran 2012/2013 berjumlah 31 siswa.Sasaran perubahan dalam penelitian ini yaitu peningkatan hasil, aktivitas dan sikap siswa terhadap pelajaran Fisika. Peneliti dapat menyimpulkan bahwa peningkatan hasil belajargetaran, gelombang dan bunyi terjadi karena kerjasama siswa selama proses pembelajaran menumbuhkan suasana yang nyaman sekaligus kompetitif. Perubahan sikap siswa terhadap pelajaran Fisika semakin baik. Ketika pembelajaran fisika mampu memberikan apa yang diharapkan oleh siswa, yaitu siswa merasa nyaman, tidak terintimidasi oleh guru dan teman karena keterbatasan yang dimilikinya, terlibat dengan teman sejawat pada saat menyelesaikan tugas-tugas yang

  4. Dhenggung Asmarandana dan Dhegung Banten: Sebuah Komparasi

    Directory of Open Access Journals (Sweden)

    Teguh -

    2013-11-01

    Full Text Available The Comparation of Dhenggung Asmarandana and Dhegung Banten. Penelitian ini membahas perbandinganantara gending Dhenggung Asmaradana gaya Surakarta dengan gending Dhegung Banten gaya Yogyakarta.Berdasarkan penelitian dapat disimpulkan bahwa kedua gending memiliki persamaan dan perbedaan. Persamaankeduanya terletak pada laras, pathet, bentuk gendhing, struktur gendhing, serta balungan gending. Sedangkanperbedaannya hanya pada nama dan garap tabuhan bonang.

  5. PERTUMBUHAN EKONOMI DAN KEBUTUHAN PANGAN DI INDONESIA

    Directory of Open Access Journals (Sweden)

    S. Rusdiana

    2017-04-01

    Full Text Available Pertumbuhan ekonomi berpengaruh terhadap kebutuhan pangan, sesuai dengan pertambahan jumah penduduk. Kebutuhan pangan di Indonesia hampir dapat dipenuhi semua, dari potensi domestik, kecuali untuk komoditas pangan asal daging impor dan kedelai yang masih mengalami defisit, sedangkan untuk beras, jagung, kacang maupun ubi, telor, daging ayam, dan susu mengalami surplus yang tinggi. Tujuan tulisan ini untuk mengetahui petumbuhan ekonomi dan kebutuhan pangan di Indonesia, sehingga dapat diatasi dengan penyediaan pangan asal pertanian dan peternakan sesuai kebutuhan. Pemerintah dapat mempertahankan dan berupaya terus memacu pembangunan ketahanan pangan, melalui program yang benar-benar mampu memperkokoh untuk ketahanan pangan, sekaligus dapat meningkatkan kesejahteraan masyarakat. Kebijakan pembangunan nasional dalam rangka mewujudkan kedaulatan pangan yang diarahkan pada peningkatan produksi pangan asal daging sapi dan tanamanm pangan beras. Tingkat pendapatan rumah tangga dapat mencerminkanmenjadi salah satu ukuran kemampuan dalam mengakses konsumsi pangan yang dibutuhkan beserta keragamannya.Pertumbuhan komoditi pangan yang paling tinggi setiap tahun adalah komoditi beras, sedangkan kontribusi daging sapi dalam memenuhi kebutuhan protein hewani menduduki urutan yang kedua setelah daging unggas. Dalam pencapaian swasembada pangan perlu difokuskan pada terwujudnya ketahanan pangan dan pengembangan teknologi pangan, diharapkan mampu memfasilitasi program pasca panen dan pengolahan hasil pertanian, secara efektif, serta mendukung kebijakan pemerintah, lebih memperhatikan masalah ketahanan pangan yang ada di Indonesia.

  6. (Crustacés, Amphipodes) dans la

    African Journals Online (AJOL)

    enseignant1

    Fadil, 2006 (Crustacés, Amphipodes) dans la source Tataw (Moyen Atlas, Maroc) ... sous-espèces ou des formes locales multiples dans toute l'Europe centrale [3 - 10]. .... le pH, et la conductivité électrique ont été réalisées sur le terrain à l'aide ...

  7. Fluorescence of ceramic color standards

    International Nuclear Information System (INIS)

    Koo, Annette; Clare, John F.; Nield, Kathryn M.; Deadman, Andrew; Usadi, Eric

    2010-01-01

    Fluorescence has been found in color standards available for use in calibration and verification of color measuring instruments. The fluorescence is excited at wavelengths below about 600 nm and emitted above 700 nm, within the response range of silicon photodiodes, but at the edge of the response of most photomultipliers and outside the range commonly scanned in commercial colorimeters. The degree of fluorescence on two of a set of 12 glossy ceramic tiles is enough to introduce significant error when those tiles have been calibrated in one mode of measurement and are used in another. We report the nature of the fluorescence and the implications for color measurement.

  8. [Ciguatera fish poisoning].

    Science.gov (United States)

    Oehler, Erwan; Bouchut, Jérémie

    2014-09-01

    Ciguatera, an ichtyosarcotoxism linked to the consumption of usually healthy coral fish is a common poisoning in the Pacific, Caribbean and Indian Ocean where it is endemic. However, increased tourism and commercial transportation of tropical fish for consumption make it an unexceptional intoxication in countries away from its endemic area. Environmental stresses such as climate changes also contribute to the expansion of its geographical area. The non-specific clinical symptomatology is characterized by the occurrence of gastrointestinal, cardiovascular, nervous and general signs few hours after eating a ciguatoxic fish. The diagnosis is clinical and relatively easy in endemic areas but much less for physicians who are rarely confronted with, which is a source of prolonged diagnostic delays and a significant increase in spending. Treatment of ciguatera is symptomatic but new treatments, still experimental, give a real hope for the future. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  9. Fish consumption and track to a fish feed formulation

    Science.gov (United States)

    Cai-Juan, Soong; Ramli, Razamin; Rahman, Rosshairy Abdul

    2015-12-01

    Strategically located in the equator, Malaysia is blessed with plenty of fish supply. The high demand in fish consumption has helped the development in the fishery industry and provided numerous jobs in the secondary sector, contributing significantly to the nation's income. A survey was conducted to understand the trend of current demands for fish for the purpose of designing a feed formulation, which is still limited in this area of study. Results showed that grouper fish in restaurants commanded a very high price compared to other species of fish. Tiger grouper gained the highest demand in most restaurants, while giant grouper had the highest price in restaurants. Due to the demand and challenges to culture this type of fish, a framework for fish feed formulation is proposed. The formulation framework when materialized could be an alternative to the use of trash fish as the feed for grouper.

  10. Soluble protein isolated from low cost fish and fish wastes

    OpenAIRE

    Lekshmy Nair, A.; Gopakumar, K.

    1982-01-01

    The method of preparation, composition, amino acid content, protein efficiency ratio and areas of possible application of water soluble protein isolates from low cost fish and fish wastes are discussed in detail in this communication.

  11. Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications.

    Science.gov (United States)

    Cui, Chenghua; Shu, Wei; Li, Peining

    2016-01-01

    Fluorescence in situ hybridization (FISH) is a macromolecule recognition technology based on the complementary nature of DNA or DNA/RNA double strands. Selected DNA strands incorporated with fluorophore-coupled nucleotides can be used as probes to hybridize onto the complementary sequences in tested cells and tissues and then visualized through a fluorescence microscope or an imaging system. This technology was initially developed as a physical mapping tool to delineate genes within chromosomes. Its high analytical resolution to a single gene level and high sensitivity and specificity enabled an immediate application for genetic diagnosis of constitutional common aneuploidies, microdeletion/microduplication syndromes, and subtelomeric rearrangements. FISH tests using panels of gene-specific probes for somatic recurrent losses, gains, and translocations have been routinely applied for hematologic and solid tumors and are one of the fastest-growing areas in cancer diagnosis. FISH has also been used to detect infectious microbias and parasites like malaria in human blood cells. Recent advances in FISH technology involve various methods for improving probe labeling efficiency and the use of super resolution imaging systems for direct visualization of intra-nuclear chromosomal organization and profiling of RNA transcription in single cells. Cas9-mediated FISH (CASFISH) allowed in situ labeling of repetitive sequences and single-copy sequences without the disruption of nuclear genomic organization in fixed or living cells. Using oligopaint-FISH and super-resolution imaging enabled in situ visualization of chromosome haplotypes from differentially specified single-nucleotide polymorphism loci. Single molecule RNA FISH (smRNA-FISH) using combinatorial labeling or sequential barcoding by multiple round of hybridization were applied to measure mRNA expression of multiple genes within single cells. Research applications of these single molecule single cells DNA and RNA FISH

  12. Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications

    Directory of Open Access Journals (Sweden)

    Chenghua Cui

    2016-09-01

    Full Text Available Fluorescence in situ hybridization (FISH is a macromolecule recognition technology based on the complementary nature of DNA or DNA/RNA double strands. Selected DNA strands incorporated with fluorophore-coupled nucleotides can be used as probes to hybridize onto the complementary sequences in tested cells and tissues and then visualized through a fluorescence microscope or an imaging system. This technology was initially developed as a physical mapping tool to delineate genes within chromosomes. Its high analytical resolution to a single gene level and high sensitivity and specificity enabled an immediate application for genetic diagnosis of constitutional common aneuploidies, microdeletion/microduplication syndromes and subtelomeric rearrangements. FISH tests using panels of gene-specific probes for somatic recurrent losses, gains and translocations have been routinely applied for hematologic and solid tumors and are one of the fastest-growing areas in cancer diagnosis. FISH has also been used to detect infectious microbials and parasites like malaria in human blood cells. Recent advances in FISH technology involve various methods for improving probe labeling efficiency and the use of super resolution imaging systems for direct visualization of intra-nuclear chromosomal organization and profiling of RNA transcription in single cells. Cas9-mediated FISH (CASFISH allowed in situ labeling of repetitive sequences and single-copy sequences without the disruption of nuclear genomic organization in fixed or living cells. Using oligopaint-FISH and super-resolution imaging enabled in situ visualization of chromosome haplotypes from differentially specified single-nucleotide polymorphism loci. Single molecule RNA FISH (smRNA-FISH using combinatorial labeling or sequential barcoding by multiple round of hybridization were applied to measure mRNA expression of multiple genes within single cells. Research applications of these single molecule single cells

  13. Penerapan Sistem Remunerasi dan Kinerja Pelayanan

    Directory of Open Access Journals (Sweden)

    Tri Wisesa Soetisna

    2015-08-01

    Full Text Available Remunerasi dapat memengaruhi motivasi pegawai sekaligus meningkatkan kinerjanya. Demikian halnya di rumah sakit sebagai institusi pelayanan kesehatan yang padat modal, sumber daya manusia serta padat ilmu dan teknologi. Penelitian ini bertujuan untuk menganalisis persepsi pegawai terhadap implementasi sistem remunerasi dan kinerja unit pelayanan bedah jantung dewasa (UPBJD di rumah sakit. Penelitian ini menggunakan pendekatan mixed methods (kuantitatif dan kualitatif. Desain penelitian kuantitatif adalah potong lintang menggunakan instrumen kuesioner self-assessment. Sedangkan desain penelitian kualitatif adalah deskriptif, dilakukan melalui focus group discussion dan telaah dokumen pada data berupa buku jadwal, buku registrasi, catatan keperawatan, dan rekam medis. Pengambilan data dilakukan pada tahun 2013 di salah satu rumah sakit di Jakarta. Responden/informan adalah staf medis fungsional, perawat, dan petugas administrasi berjumlah 29 orang. Data dianalisis secara univariat (metode kuantitatif, dan content analysis (metode kualitatif. Hasil penelitian menunjukkan bahwa sebagian besar staf medis fungsional dan perawat tidak puas (71,2% dengan beberapa hal dalam penerapan sistem remunerasi, seperti pada sistem penggajian dan penentuan grading. Terlihat kinerja unit pelayanan bedah jantung dewasa mengalami kenaikan setiap tahun sebelum dan setelah penerapan sistem remunerasi. Diharapkan agar rumah sakit ini dapat memperbaiki sistem remunerasi yang sesuai ketentuan kebijakan dan menyusun formulasi insentif dan bonus yang lebih sesuai dengan kondisi saat ini serta perlu dilakukan sosialisasi yang tepat dan evaluasi secara berkala. Implementation of Remuneration System and Service Performance Remuneration can influence worker`s motivation, and improve their performance. Likewise in hospital as capital-intensive, human resources-intensive as well as knowledge and technology-intensive health care institution. This study aimed to analyze employee

  14. First molecular identification of the transgene red fluorescent protein (RFP in transgenic ornamental zebrafish (Danio rerio introduced in Peru

    Directory of Open Access Journals (Sweden)

    Carlos Scotto

    2013-09-01

    Full Text Available In this paper the transgenic fluorescent red, orange and pink zebra fish (Danio rerio, found in local aquariums in Peru, were identified using the PCR technique to amplify the transgene RFP sea anemone belonging to Discosoma spp. The gene expression of the red fluorescent protein (RFP transgene was found to determine different gradients-of-bioluminescence (shades in color in each GMO fish analyzed. We performed sequence analysis of the two variants of the RFP along with six variants of the existing fluorescent protein GFP from the Genbank, this could help identify quickly if they are new genes or variants thereof as these novel fluorescent proteins may be introduced in aquatic GMO in the future. Thus, developing and improving biosecurity measures through its timely detection at the molecular genetic level.

  15. HUBUNGAN NONMONOTONIK ANTARA KONTROL DAN KINERJA PERUSAHAAN

    Directory of Open Access Journals (Sweden)

    Gede Juliarsa

    2012-07-01

    Full Text Available ABSTRAK Dalam rerangka agency theory, manajemen perusahaan merupakan agent yang harus bertanggung jawab kepada pemegang saham sebagai principal. Agent diberikan tanggung jawab untuk mengelola aset principal untuk dapat dikelola secara ekonomis. Studi ini bertujuan untuk mengetahui hubungan antara kinerja perusahaan dengan kepemilikan manajemen dan ukuran perusahaan. Studi ini menguji 46 kinerja perusahaan tahun 2001 terhadap perusahaan go public yang terdaftar di Bursa Efek Jakarta.  Data diperoleh dari Indonesian Capital Market Directory 2002. Pengujian hubungan antara kinerja perusahaan menggunakan Economic Value Added, kepemilikan manajemen, dan ukuran perusahaan dilakukan dengan analisis regresi berganda dan korelasi. Hasil pengujian menunjukkan bahwa hubungan antara kinerja perusahaan, ukuran perusahaan, dan kepemilikan manajemen adalah tidak monotonik, tetapi secara signifikan curvilinear. Temuan ini sesuai dengan penelitian yang dilakukan oleh Fogelberg dan Griffth (2000.   Kata kunci: economic value added, kepemilikan manajemen, kinerja,ukuran perusahaan, teori agensi

  16. Monosomy 3 by FISH in uveal melanoma: variability in techniques and results.

    Science.gov (United States)

    Aronow, Mary; Sun, Yang; Saunthararajah, Yogen; Biscotti, Charles; Tubbs, Raymond; Triozzi, Pierre; Singh, Arun D

    2012-09-01

    Tumor monosomy 3 confers a poor prognosis in patients with uveal melanoma. We critically review the techniques used for fluorescence in situ hybridization (FISH) detection of monosomy 3 in order to assess variability in practice patterns and to explain differences in results. Significant variability that has likely affected reported results was found in tissue sampling methods, selection of FISH probes, number of cells counted, and the cut-off point used to determine monosomy 3 status. Clinical parameters and specific techniques employed to report FISH results should be specified so as to allow meta-analysis of published studies. FISH-based detection of monosomy 3 in uveal melanoma has not been performed in a standardized manner, which limits conclusions regarding its clinical utility. FISH is a widely available, versatile technology, and when performed optimally has the potential to be a valuable tool for determining the prognosis of uveal melanoma. Copyright © 2012 Elsevier Inc. All rights reserved.

  17. Seminar dan Wokshop Jurnalistik Anak SMA “Yournalism”

    OpenAIRE

    T.R, Amal Gamasi; Nugroho, Adi; Setyabudi, Djoko

    2013-01-01

    PENDAHULUANMasyarakat Indonesia saat ini merupakan masyarakat informasi yangmenghabiskan sebagian besar waktunya dengan media komunikasi dan menggunakanteknologi informasi seperti ponsel dan komputer maupun laptop. Mereka akan mudahmelakukan pertukaran data informasi karena saat ini,mengingat konvergensi mediatidak hanya mengubah basis data dan medium yang menyalurkannya, tetapi jugasecara keseluruhan mengubah proses produksi, pengolahan, dan distribusi informasisehingga media-media seperti k...

  18. Detection of dengue group viruses by fluorescence in situ hybridization

    Directory of Open Access Journals (Sweden)

    Raquin Vincent

    2012-10-01

    Full Text Available Abstract Background Dengue fever (DF and dengue hemorrhagic fever (DHF represent a global challenge in public health. It is estimated that 50 to 100 million infections occur each year causing approximately 20,000 deaths that are usually linked to severe cases like DHF and dengue shock syndrome. The causative agent of DF is dengue virus (genus Flavivirus that comprises four distinct serotypes (DENV-1 to DENV-4. Fluorescence in situ hybridization (FISH has been used successfully to detect pathogenic agents, but has not been implemented in detecting DENV. To improve our understanding of DENV infection and dissemination in host tissues, we designed specific probes to detect DENV in FISH assays. Methods Oligonucleotide probes were designed to hybridize with RNA from the broadest range of DENV isolates belonging to the four serotypes, but not to the closest Flavivirus genomes. Three probes that fit the criteria defined for FISH experiments were selected, targeting both coding and non-coding regions of the DENV genome. These probes were tested in FISH assays against the dengue vector Aedes albopictus (Diptera: Culicidae. The FISH experiments were led in vitro using the C6/36 cell line, and in vivo against dissected salivary glands, with epifluorescence and confocal microscopy. Results The three 60-nt oligonucleotides probes DENV-Probe A, B and C cover a broad range of DENV isolates from the four serotypes. When the three probes were used together, specific fluorescent signals were observed in C6/36 infected with each DENV serotypes. No signal was detected in either cells infected with close Flavivirus members West Nile virus or yellow fever virus. The same protocol was used on salivary glands of Ae. albopictus fed with a DENV-2 infectious blood-meal which showed positive signals in the lateral lobes of infected samples, with no significant signal in uninfected mosquitoes. Conclusion Based on the FISH technique, we propose a way to design and use

  19. SPECIES INVENTORY OF AMPHIBIOUS FISH IN INTERTIDAL ZONE OF NGRENEHAN, NGOBARAN AND NGUYAHAN COASTELS, GUNUNG KIDUL, YOGYAKARTA

    Directory of Open Access Journals (Sweden)

    Sukiya Sukiya

    2015-10-01

    Full Text Available Tropical region is high on faunal diversity including vertebrates and invertebrates. Research on diversity of amphibious fish in Intertidal zone of  Yogyakarta southern coast is still limited so there is no complete records regarding these unique fish. This research aimed to know the diversity of amphibious fish in three beaches of Yogyakarta southern coast, namely pantai Ngrenehan, Ngobaran dan Nguyahan. Fish sampling were conducted using random sampling method combined with observational method. Fish were collected using small nets then identified to species rank. The total length of each fish were measured and recorded. Few individuals of the same species were sacrificed as voucher specimens. The result showed that in pantai Ngrenehan, Ngobaran, and Nguyahan, five different species of amphibius fish were found, which are Cabillus lacertops, Bathygobius fuscus, Enneapterygus sp., Blenniella cyanostigma, and Blenniella caudolineata. Cabillus lacertops was found in all of sampling locations. Enneapterygus sp. was found in pantai Nguyahan while Bathigobius fuscus, and Blenniella caudolineata can only be found in pantai Ngrenehan. Further studies regarding the adaptation, ecological features, taxonomy and biological reproduction of Yogyakarta southern coast’s  amphibious fish are still needed.   Keywords: Inventarization, amphbious fish, intertidal zone, Yogyakarta southern coast

  20. Fish parasites, fish food, and the marine environment | Nnadi ...

    African Journals Online (AJOL)

    The paper addresses the incontrovertible fact that fish and fish products have historically been a reliable supplier of protein, in particular, and food, in general for humans. Seventy to a hundred metric tons arc caught each year since the early seventies. Fish protein represents about twenty five percent of the total animal ...

  1. Fish Commoditization: Sustainability Strategies to Protect Living Fish

    Science.gov (United States)

    Lam, Mimi E.; Pitcher, Tony J.

    2012-01-01

    The impacts of early fishing on aquatic ecosystems were minimal, as primitive technologies were used to harvest fish primarily for food. As fishing technology grew more sophisticated and human populations dispersed and expanded, local economies transitioned from subsistence to barter and trade. Expanded trade networks and mercantilization led to…

  2. Economic differential of integrated fish, rice cum piggery and fish ...

    African Journals Online (AJOL)

    Fish, rice cum piggery and fish, rice cum poultry production integration platforms were set up to perfect the ideas of integrated fish farming in Sierra Leone. Water quality parameters measured biweekly included: dissolved oxygen, water temperature, pH, water hardness, water alkalinity, ammonia, nitrate, BOD and nitrite.

  3. Survival and growth responses of snakehead fish Channa striata Bloch. juvenile in aerated and unaerated acid sulfate water

    Directory of Open Access Journals (Sweden)

    , Purnamawati

    2017-07-01

    Full Text Available ABSTRACT  The aim of the research was to analyze survival rate, specific growth rate, albumin, and feed efficiency and physiological (blood glucose, cortisol, dan haemoglobin responses of snake head fish juvenil that reared at aerated and unaerated of tidal land water have been conducted in the laboratory. Experiments using completely randomized design with aerated and unaerated as a treatment, and each treatment has twelve replications. The snakehead fish juvenil wich length 2.4±0.2 cm and weight of 0.21±0.05 g reared in the aquarium that are size 30×25×35 cm (water volume 25 L with a stocking density 2 juvenile/L, for 40 days. The fishes were fed with commercial feed with protein content about 40%, feeding two times a day (morning and afternoon were at satiation. Replacement of water done every two days about 10% of the total water volume in the aquarium. The results showed that unaerated median significantly affected to biometric and physiological response of juvenile of snake head fish. The media un-aerated gives the best results shown by the higher value of survival (92%, specific growth rate (6.73%/ day, feed efficiency (78.22%, protein retention (41.91%, energy retention (30.81% value of albumin (6.60 g/100 mL and the haemoglobin (5.58 g/dL, and have the lowest value of cortisol (21.49 ng/L and blood glucose (43.36 mg/100 mL. Keywords: acid sulfate water, growth rate, aeration, Channa striata   ABSTRAK  Penelitian ini bertujuan untuk menganalisis respons kelangsungan hidup, pertumbuhan spesifik, albumin, dan efisiensi pakan dan fisiologis (kortisol, glukosa darah, dan hemoglobin juvenil ikan gabus yang dipelihara dengan dan tanpa aerasi pada media air rawa pasang surut. Penelitian menggunakan rancangan acak lengkap dengan aerasi dan tanpa aerasi sebagai perlakuan, dan masing-masing perlakuan memiliki 12 ulangan. Juvenil ikan gabus berukuran panjang 2,4±0,3 cm dan bobot 0,21±0,03 g dipelihara dalam akuarium 30×25×35 cm (volume

  4. Fast-FISH Detection and Semi-Automated Image Analysis of Numerical Chromosome Aberrations in Hematological Malignancies

    Directory of Open Access Journals (Sweden)

    Arif Esa

    1998-01-01

    Full Text Available A new fluorescence in situ hybridization (FISH technique called Fast-FISH in combination with semi-automated image analysis was applied to detect numerical aberrations of chromosomes 8 and 12 in interphase nuclei of peripheral blood lymphocytes and bone marrow cells from patients with acute myelogenous leukemia (AML and chronic lymphocytic leukemia (CLL. Commercially available α-satellite DNA probes specific for the centromere regions of chromosome 8 and chromosome 12, respectively, were used. After application of the Fast-FISH protocol, the microscopic images of the fluorescence-labelled cell nuclei were recorded by the true color CCD camera Kappa CF 15 MC and evaluated quantitatively by computer analysis on a PC. These results were compared to results obtained from the same type of specimens using the same analysis system but with a standard FISH protocol. In addition, automated spot counting after both FISH techniques was compared to visual spot counting after standard FISH. A total number of about 3,000 cell nuclei was evaluated. For quantitative brightness parameters, a good correlation between standard FISH labelling and Fast-FISH was found. Automated spot counting after Fast-FISH coincided within a few percent to automated and visual spot counting after standard FISH. The examples shown indicate the reliability and reproducibility of Fast-FISH and its potential for automatized interphase cell diagnostics of numerical chromosome aberrations. Since the Fast-FISH technique requires a hybridization time as low as 1/20 of established standard FISH techniques, omitting most of the time consuming working steps in the protocol, it may contribute considerably to clinical diagnostics. This may especially be interesting in cases where an accurate result is required within a few hours.

  5. Biofilm biodiversity presented by fluorescent in situ hybridisation

    Directory of Open Access Journals (Sweden)

    Wolf Mirela

    2017-01-01

    Full Text Available Numerous microorganisms may be present in the water distribution system. This is associated with the imperfection of purification processes, or secondary water pollution. Not only it results in the deterioration of water quality parameters, but it also increases threat of epidemiological problems. The water that is biologically unstable creates ideal conditions for colonization of the microorganisms to the inner surface of pipelines which may form biofilm. The key issue, enabling prevention and control of the impact of the development of biofilms, is to assess the biodiversity of microbiocenosis. In order to obtain comprehensive characteristics of microorganisms communities on a particular substrate, it is necessary to combine several techniques. Further analysis using molecular biology methods are usually after traditional methods of assessing the microbiological quality of water. Standard methods do not reflect the actual species composition, because they are targeted at the bacteria that can be isolated and cultured in the laboratory. Conventional methods are capable of detecting less than 10% of the organisms in the sample. In order to study the biodiversity of organisms inhabiting a biofilm (apart from the conventional methods analyses of the diversity of nucleic acids should be used. The first method could be the polymerase chain reaction (PCR and denaturing gradient gel electrophoresis (DGGE. Another way may be fluorescence in situ hybridization, which allows to detect determined DNA sequence using specially labeled oligonucleotide probes. Visualization of the material is performed using a fluorescence microscope. The main purpose of this article is to present rapid and precise identification groups of microorganisms in their natural habitat in biofilm using fluorescent in situ hybridization method (FISH . FISH method can be successfully used to visualize these microorganisms, which show difficulties in culturing, as well as to provide

  6. Struktur Komunitas Ikan Karang di Perairan Kendari (Community Structure of Coral Reef Fishes at Kendari Waters

    Directory of Open Access Journals (Sweden)

    Muhammad Adrim

    2012-09-01

    Full Text Available Keberadaan ikan karang merupakan salah satu bioindikator terhadap kondisi terumbu karang yang baik. Penelitian ikan karang di perairan Kendari bertujuan untuk mengetahui komposisi jenis, kelimpahan, sebaran, dan struktur komunitas ikan karang di perairan tersebut. Pengumpulan data dilakukan bulan Juli 2011 pada lima lokasi di bagian utara dan selatan Kendari. Data dihimpun dengan menggunakan teknik Underwater Visual Census (UVC dan metode transek (Line Intersept Transect, LIT dengan peralatan SCUBA. Total jenis ikan karang terkumpul sebanyak 111 jenis yang mewakili 24 famili, terdiri dari 31 jenis ikan target (ikan konsumsi, 17 jenis ikan indikator (indicator species, dan kelompok major 63 jenis. Kelornpok ikan pangan (target yang dominan; Caesio cuning, Siganus vulpinus dan Ctenochaetus striatus. Jenis yang paling dominan dari ikan indikator adalah Chaetodon octofasciatus. Sedangkan kelompok lainnya (major yang dominan adalah Pomacentrus smithii, Chrysiptera rollandi, Chrysiptera springeri, dan Pomacentrus alexanderae. Nilai Indeks keanekaragaman berkisar 1,36– 3,23. Indeks dominasi Margalef (d berkisar 4,74–8,66. Indeks kemerataan Pielou (J’=H’/logeS diperololeh pada kisaran 0,38–0,81 . Hasil analisis kluster pada matrik kesamaan Bray-Curtis 37 % diperoleh dendrograrn yang menunjukkan dua pengelompokan stasiun. Berdasarkan ordinasi sampel dengan MDS diperoleh dari kesamaan (stress= 0 dengan jelas menunjukkan dua komunitas yang berbeda. Hasil penelitian ini diharapkan dapat menjadi masukan sebagai data dasar untuk pengelolaan daerah pesisir bagi pemerintahan daerah (PEMDA. Kata kunci: ikan karang, struktur komunitas, indeks ekologi, perairan Kendari. Coral reef Fishes is one of bio-indicators for good condition coral reef ecosystem. A study on coral reef fishes in the Kendari waters was aimed to find out species composition, abundance, distribution and community structure of coral reef fishes in that area. The study was conducted in

  7. TUGAS DAN WEWENANG PUSAT PELAPORAN DAN ANALISIS TRANSAKSI KEUANGAN (PPATK DALAM PEMBERANTASAN TINDAK PIDANA PENCUCIAN UANG

    Directory of Open Access Journals (Sweden)

    Johari Johari

    2014-03-01

    Full Text Available Tugas dan wewenang Pusat Pelaporan dan Analisis Transaksi Keuangan (PPATK terdapat di dalam Pasal 26 dan Pasal 27 Undang-Undang No. 25 Tahun 2003 tentang Tindak Pencucian Uang. Berdasarkan ketentuan tersebut, tugas dan wewenang PPATK tersebut bertujuan untuk mendeteksi terjadinya tindak pidana pencucian uang, dan membantu penegakan hukum yang berkaitan dengan pencucian uang, termasuk tindak pidana asal yang melahirkannya (predicate offences. Namun, Peranan PPATK akan berjalan secara efektif apabila aparat penegak hukum seperti Kepolisian, Kejaksaan, Pengadilan, Bea dan Cukai, para regulator seperti Bank Indonesia, Departemen Keuangan, Badan Pengawas Pasar Modal serta Penyedia Jasa Keuangan, industri perbankan, asuransi, perusahaan pembiayaan, dana pensiun, perusahaan efek, pengelola reksadana, media massa, masyarakat bekerjasama secara terorganisir dan terpadu dalam pemberantasan tindak pencucian uang di Indonesia. Dengan kewenangan yang dimilikinya, PPATK dapat mengejar hasil dari kejahatan, apabila hasil kejahatan tersebut dapat dikejar dan disita maka negara dengan sendirinya akan mengurangi tindak kejahatan itu sendiri. Kata kunci : Pencucian uang, tindak pidana pencucian uang (money laundering, kejahatan terorganisir, dan Pusat Pelaporan dan Analisis Transaksi Keuangan (PPATK.

  8. PENGARUH VARIETAS DAN SISTEM BUDIDAYA TERHADAP PERTUMBUHAN, PRODUKSI, DAN KANDUNGAN GIZI JAGUNG (ZEA MAYS L.

    Directory of Open Access Journals (Sweden)

    Yukarie Ayu Wulandari

    2016-06-01

    Full Text Available Jagung merupakan bahan pangan yang berpotensi untuk dikembangkan sebagai pangan lokal, pakan, dan bahan baku industri. Mengingat pentingnya jagung, maka perlu adanya upaya untuk peningkatan produktivitasnya. Penelitian bertujuan untuk mengetahui pengaruh varietas dan sistem budidaya terhadap pertumbuhan, produksi, dan kandungan gizi jagung. Penelitian menggunakan Rancangan Petak Terbagi dengan petak utama varietas (Srikandi Putih-1 dan Srikandi Kuning-1 dan anak petak sistem budidaya (organik dan inorganik yang diulang lima kali. Hasil menunjukkan bahwa varietas Srikandi Putih-1 memberikan hasil tertinggi pada tinggi tanaman, jumlah daun, luas daun, lingkar batang, pati, dan vitamin A. Varietas Srikandi Kuning-1 memberikan hasil tertinggi pada produksi, protein dan lemak. Sistem budidaya inorganik memberikan hasil tertinggi pada tinggi tanaman, jumlah daun, panjang tongkol, bobot bersih tongkol, bobot pipilan kering, bobot 100 butir, konversihasil/ha, patidan protein. Interaksi varietas Srikandi Putih-1 dengan sistem budidaya organik memberikan hasil tertinggi terhadap jumlah daun, lingkar batang dan kandungan vitamin A. Varietas Srikandi Kuning-1 yang dibudidayakan secara inorganik memberikan hasil tertinggi terhadap bobot bersih tongkol, bobot pipilan kering, bobot 100 butir, konversi hasil ha-1 dan kandungan protein. Kandungan pati tertinggi pada varietas Srikandi Putih-1 yang dibudidayakan secara inorganik, sedangkan kandungan lemak tertinggi pada varietas Srikandi Kuning-1 yang dibudidayakan secara organik.

  9. Peran Mitra Strategis dan Agen Perubahan dalam Manajemen Talenta dan Kinerja Manajer

    Directory of Open Access Journals (Sweden)

    Ida Ketut Kusumawijaya

    2014-06-01

    Full Text Available Abstract  The purposes of the study are to analyze: (1 characteristic of strategic partner and change agent roles, talent management and managers performance, (2 influence of strategic partner and change agent roles to talent management and its impact to managers performance. Four hundred hotel managers in Bali were participated in this study by using proportional random sampling. Data were analyzed using descriptive analysis and SEM. The result shows that: (1 strategic partner and change agent role, talent management and managers performance are in enough category, (2 positive and significant effect between strategic partner and change agent roles to talent management and its impact to managers performance. Keywords:Strategic Partner Role, Change Agent Role, Talent Management, Managers Performance.AbstrakTujuan penelitian ini adalah untuk menganalisis: (1 karakteristik dari mitra strategis dan peran agen perubahan, manajemen bakat dan kinerja manajer, (2 pengaruh mitra strategis dan peran agen perubahan untuk manajemen bakat dan dampaknya terhadap kinerja manajer. Sebanyak empat ratus manajer hotel di Bali berpartisipasi dalam penelitian ini dengan menggunakan proporsional random sampling. Data dianalisis menggunakan analisis deskriptif dan SEM. Hasil penelitian menunjukkan bahwa: (1 mitra strategis dan peran agen perubahan, manajemen bakat dan kinerja manajer termasuk dalam kategori cukup, (2 pengaruh positif dan signifikan antara mitra strategis dan peran agen perubahan untuk manajemen bakat dan dampaknya terhadap kinerja manajer.Keywords: Peran Mitra Strategis, Peran Agen Perubahan, Manajemen Talenta, kinerja Manajer.

  10. Temperature - Live Hauling of Fish

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In certain markets, live fish can be sold for substantially higher prices than fresh dressed fish. A significant live-haul industry has developed in the U.S. and...

  11. LCA of Danish fish products

    DEFF Research Database (Denmark)

    Thrane, Mikkel

    2006-01-01

    The article presents the main results from a PhD dissertation about environmental impacts from Danish fish products.......The article presents the main results from a PhD dissertation about environmental impacts from Danish fish products....

  12. KLA - Live Hauling of Fish

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In certain markets, live fish can be sold for substantially higher prices than fresh dressed fish. A significant live-haul industry has developed in the U.S. and...

  13. Pickering nuclear fish diversion net

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, J.; Lew, A. [Ontario Power Generation, Toronto, Ontario (Canada)

    2013-07-01

    Pickering Fish Diversion Net - An Engineered Environmental Solution that has significantly reduced fish impingement at the Pickering Nuclear Facility. Note: As a recent urgent request/discussed by Mark Elliot, CNE-OPG and Jacques Plourde, CNS.

  14. Human cDNA mapping using fluorescence in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Korenberg, J.R.

    1993-03-04

    Genetic mapping is approached using the techniques of high resolution fluorescence in situ hybridization (FISH). This technology and the results of its application are designed to rapidly generate whole genome as tool box of expressed sequence to speed the identification of human disease genes. The results of this study are intended to dovetail with and to link the results of existing technologies for creating backbone YAC and genetic maps. In the first eight months, this approach generated 60--80% of the expressed sequence map, the remainder expected to be derived through more long-term, labor-intensive, regional chromosomal gene searches or sequencing. The laboratory has made significant progress in the set-up phase, in mapping fetal and adult brain and other cDNAs, in testing a model system for directly linking genetic and physical maps using FISH with small fragments, in setting up a database, and in establishing the validity and throughput of the system.

  15. KAJIAN ASPEK FISIKA, KIMIA, DAN BIOLOGI PERAIRAN SITU RAWABEBEK, KARAWANG, DALAM RANGKA PENGELOLAAN PERIKANAN BERBASIS BUDI DAYA

    Directory of Open Access Journals (Sweden)

    Bambang Gunadi

    2016-11-01

    Full Text Available Penelitian tentang aspek-aspek fisika, kimia, dan biologi perairan bekas galian pasir telah dilakukan di Situ Rawabebek Kabupaten Karawang. Tujuan penelitian adalah untuk mendapatkan data dasar bagi penyusunan model pengelolaan perikanan di perairan bekas galian pasir berdasarkan prinsip perikanan berbasis budi daya (culture-based fisheries, CBF. Hasil pengamatan menunjukkan bahwa fluktuasi air di Situ Rawabebek mencapai hingga 1,90 m dan sangat dipengaruhi oleh ketinggian air Sungai Citarum. Nilai semua parameter kualitas air berada dalam kisaran yang layak untuk mendukung kehidupan ikan sementara kadar logam berat berada dalam ambang aman untuk kehidupan organisme akuatik termasuk ikan dan kesehatan manusia. Indeks keragaman plankton (Indeks Shanon-Wiener pada setiap stasiun dan musim pengamatan berkisar antara 3,95—4,52 yang berarti bahwa komunitas plankton di Situ Rawabebek stabil dan perairannya dalam kondisi belum tercemar. Jenis ikan yang teramati untuk mengetahui pola reproduksinya di Situ Rawabebek antara lain lalawak (Puntius bramoides, paray (Rasbora argyrotaenia, dan seren (Cyclocheilichthys apogon dengan tingkat Indeks Kematangan Gonad (IKG masing-masing 3,43%; 12,82%; dan 3,95%. Dari analisis terhadap kondisi fisika, kimia, dan biologinya disimpulkan bahwa perairan bekas galian pasir Situ Rawabebek cukup layak untuk pengembangan perikanan berbasis budi daya. A study on the physical, chemical, and biological aspects of the abandoned sand mining reservoir was conducted in Rawabebek Reservoir (Karawang Regency, West Java. This study aimed to develop a model of fisheries management in the reservoir based on the culture based fisheries (CBF system. The study showed that the fluctuation of water level in the Rawabebek Reservoir was monitored up to 1.90 m and highly influenced by the water level of Citarum River. The average value of all water quality parameters and heavy metals were in the suitable range for aquatic organism, fish and

  16. Immunohistochemistry and alternative FISH testing in breast cancer with HER2 equivocal amplification.

    Science.gov (United States)

    Agersborg, Sally; Mixon, Christopher; Nguyen, Thanh; Aithal, Sramila; Sudarsanam, Sucha; Blocker, Forrest; Weiss, Lawrence; Gasparini, Robert; Jiang, Shiping; Chen, Wayne; Hess, Gregory; Albitar, Maher

    2018-03-22

    While HER2 testing is well established in directing appropriate treatment for breast cancer, a small percentage of cases show equivocal results by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Alternative probes may be used in equivocal cases. We present a single community-based institution's experience in further evaluating these cases. Between 2014 and 2016, 4255 samples were submitted for HER2 amplification testing by alternative probes, TP53, RAI1, and RARA. Of the patients tested by FISH, 505/3908 (12.9%) also had IHC data. Most (73.9%) FISH equivocal cases remained equivocal after IHC testing. However, 50.5% of equivocal cases were classified as HER2 amplified by alternative probes. Most cases were positive by more than one probe: 78% of positive cases by RAI1 and 73.9% by TP53. There was a significant difference between IHC and FISH alternative testing (p alternative FISH testing. Available data showed that 41% of patients were treated with palbociclib and were positive by alternative FISH. The prevalence of double HER2 equivocal cases and the discrepancy between IHC and alternative FISH testing suggest that FISH alternative testing using both RAI1 and TP53 probes is necessary for conclusive classification. Because almost half of FISH equivocal cases converted to HER2 amplified upon alternative testing, clinical studies to determine the benefit of anti-HER2 therapy in these patients are urgently needed.

  17. Guidelines for Eating Fish that Contain Mercury

    Science.gov (United States)

    Learn about how to minimize exposure to methylmercury while eating fish. Read about fish advisories, how to use them to consume fish safely, and use the national fish advisories locator to find them in an area near you.

  18. COMBO-FISH Enables High Precision Localization Microscopy as a Prerequisite for Nanostructure Analysis of Genome Loci

    Directory of Open Access Journals (Sweden)

    Rainer Kaufmann

    2010-10-01

    Full Text Available With the completeness of genome databases, it has become possible to develop a novel FISH (Fluorescence in Situ Hybridization technique called COMBO-FISH (COMBinatorial Oligo FISH. In contrast to other FISH techniques, COMBO-FISH makes use of a bioinformatics approach for probe set design. By means of computer genome database searching, several oligonucleotide stretches of typical lengths of 15–30 nucleotides are selected in such a way that all uniquely colocalize at the given genome target. The probes applied here were Peptide Nucleic Acids (PNAs—synthetic DNA analogues with a neutral backbone—which were synthesized under high purity conditions. For a probe repetitively highlighted in centromere 9, PNAs labeled with different dyes were tested, among which Alexa 488Ò showed reversible photobleaching (blinking between dark and bright state a prerequisite for the application of SPDM (Spectral Precision Distance/Position Determination Microscopy a novel technique of high resolution fluorescence localization microscopy. Although COMBO-FISH labeled cell nuclei under SPDM conditions sometimes revealed fluorescent background, the specific locus was clearly discriminated by the signal intensity and the resulting localization accuracy in the range of 10–20 nm for a detected oligonucleotide stretch. The results indicate that COMBO-FISH probes with blinking dyes are well suited for SPDM, which will open new perspectives on molecular nanostructural analysis of the genome.

  19. PENGARUH CARA PENGAWETAN TERHADAP KOMPOSISI KIMIA DAN EFISIENSI DALAM BENTUK HAY DAN SILASE PADA DAUN 16 PROVENAN GAMAL (GLIRICIDIA SEPIUM

    Directory of Open Access Journals (Sweden)

    A. W. Puger

    2012-09-01

    Full Text Available RINGKASAN Percobaan yang bertujuan untuk mengetahui pengaruh cara pengawetan terhadap kandungan zat-zat makanan dan efisiensinya pada daun 16 provenan gamal telah dilaksanakan selama 3 bulan. Penelitian menggunakan rancangan acak lengkap yang terdiri atas 3 perlakuan pengawetan (gamal segar, gamal hay, dan gamal silase dan 3 blok sebagai ulangan. Setiap perlakuan terdiri atas 16 provenan, yaitu 6 dari Mexico (M, 4 dari Guatemala (G, dan satu provenan masing-masing dari Colombia (C, Indonesia (I, Nicaragua (N, Panama (P, Costa Rica (R, dan Venezuela (V. Sampel setiap daun (helai dan tangkai provenan sebelum dan setelah diawetkan dianalisis kandungan zat-zat makanannya dan dihitung efisiensinya setelah dibuat hay dan silase. Kandungan DM dan CP dari hay adalah tertinggi (P0,05 setelah diawetkan menjadi hay dan silase, sedangkan efisiensi DM, OM, dan CP dari hay lebih tinggi (P<0,05 daripada silase. Provenan P13, R12, dan M34 mengandung zat-zat makanan lebih tinggi; sementara provenan G14 dan G17 lebih efisien bila diawetkan dalam bentuk hay dan silase. Pada pertanian lahan kering pengawetan gamal dalam bentuk hay lebih efektif dan efisien jika dibandingkan dengan dalam bentuk silase.

  20. How to Represent a Fish?

    Directory of Open Access Journals (Sweden)

    Elspeth Probyn

    2017-05-01

    Full Text Available This article canvasses a broad range of fish representations across several disciplines. It asks what cultural studies can learn from scientific representation of fish, and argues that in turn cultural studies can be a nuanced understanding of the work of images. The objective of the article is to open debate about fish and their sustainability beyond discrete disciplines and/or ideologies. This, it is argued, is crucial if we are to go beyond a simplified cultural politics of fish.

  1. Fish Oil in Diabetic Nephropathy

    DEFF Research Database (Denmark)

    Rossing, Peter; Hansen, Birgitte V.; Nielsen, Flemming S.

    1996-01-01

    OBJECTIVE: Recent studies in nondiabetic kidney diseases suggest that dietary supplementation with n-3 polyunsaturated fatty acids (fish oil) may have beneficial effects on albuminuria, kidney function, arterial blood pressure, and dyslipidemia. Therefore, we evaluated the long-term effect of fish...... in the fish oil compared with the placebo group. CONCLUSIONS: Our study does not suggest that fish oil has beneficial effects on albuminuria, kidney function, blood pressure, and dyslipidemia in normotensive IDDM patients suffering from diabetic nephropathy....

  2. High resolution FISH on super-stretched flow-sorted plant chromosomes.

    NARCIS (Netherlands)

    Valárik, M.; Bartos, J.; Kovarova, P.; Kubalakova, M.; Jong, de J.H.S.G.M.; Dolezel, J.

    2004-01-01

    A novel high-resolution fluorescence in situ hybridisation (FISH) strategy, using super-stretched flow-sorted plant chromosomes as targets, is described. The technique that allows longitudinal extension of chromosomes of more than 100 times their original metaphase size is especially attractive for

  3. The development of FISH tools for genetic, phylogenetic and breeding studies in tomato (Solanum lycopersicum)

    NARCIS (Netherlands)

    Szinay, D.

    2010-01-01

    In this thesis various fluorescence in situ hybridization (FISH) technologies are described to support genome projects, plant breeding and phylogenetic analysis on tomato (Solanum lycopersicum, 2n=24). Its genome is 980 Mb and only 30 % are single copy sequences, which are mostly found in the

  4. 10p Duplication characterized by fluorescence in situ hybridization

    Energy Technology Data Exchange (ETDEWEB)

    Wiktor, A.; Feldman, G.L.; Van Dyke, D.L.; Kratkoczki, P.; Ditmars, D.M. Jr. [Henry Ford Hospital, Detroit, MI (United States)

    1994-09-01

    We describe a patient with severe failure to thrive, mild-moderate developmental delay, cleft lip and palate, and other anomalies. Routine cytogenetic analysis documented a de novo chromosome rearrangement involving chromosome 4, but the origin of the derived material was unknown. Using chromosome specific painting probes, the karyotype was defined as 46,XY,der(4)t(4;10)(q35;p11.23). Characterization of the dup(10p) by fluorescence in situ hybridization (FISH) analysis provides another example of the usefulness of this technology in identifying small deletions, duplications, or supernumerary marker chromosomes. 19 refs., 4 figs.

  5. Fluorescing macerals from wood precursors

    Energy Technology Data Exchange (ETDEWEB)

    Stout, S A; Bensley, D F

    1987-01-01

    A preliminary investigation into the origin of wood-derived macerals has established the existence of autofluorescent maceral precursors in the secondary xylem of swamp-inhabiting plant species. The optical character and fluorescent properties of microtomed thin-sections of modern woods from the Florida Everglades and Okefenokee Swamp, Georgia are compared to the character and properties of their peatified equivalents from various Everglades and Okefenokee peat horizons and their lignitic equivalents from the Brandon lignite of Vermont and the Trail Ridge lignitic peat from northern Florida. The inherent fluorescence of woody cell walls is believed to be caused by lignin though other cell wall components may contribute. The fluorescence spectra for several wood and cell types had a ..gamma../sub m//sub a//sub x/ of 452 nm and Q value of 0.00. The color as observed in blue light and the spectral geometry as measured in UV light of peatified and lignitic woody cell walls (potential textinites) may change progressively during early coalification. Cell wall-derived maceral material is shown to maintain its fluorescing properties after being converted to a structureless material, perhaps a corpohuminite or humodetrinite precursor. Fluorescing xylem cell contents, such as condensed tannins or essential oils, can maintain the fluorescent character through early coalification. Xylem cell walls and xylem cell contents are shown to provide fluorescing progenitor materials which would not require subsequent infusion with 'lipid' materials to account for their fluorescence as phytoclast material or as macerals in coal. 35 references.

  6. Assessing Photosynthesis by Fluorescence Imaging

    Science.gov (United States)

    Saura, Pedro; Quiles, Maria Jose

    2011-01-01

    This practical paper describes a novel fluorescence imaging experiment to study the three processes of photochemistry, fluorescence and thermal energy dissipation, which compete during the dissipation of excitation energy in photosynthesis. The technique represents a non-invasive tool for revealing and understanding the spatial heterogeneity in…

  7. The fish egg microbiome

    NARCIS (Netherlands)

    Liu, Y.

    2016-01-01

    Y. Liu

    Prof. dr. F. Govers (promotor); Prof. dr. J.M. Raaijmakers (promotor); Dr. I. de Bruijn (co-promotor); Wageningen University, 13 June 2016, 170 pp.

    The fish egg microbiome: diversity and activity against the oomycete pathogen

  8. De fiscale fishing expedition

    NARCIS (Netherlands)

    Poelmann, E.

    2016-01-01

    In de fiscale literatuur en/of mediaberichten duikt met enige regelmaat de term ‘fishing expedition’ op. De term wordt in rechterlijke uitspraken en in beleid genoemd, in nationale en internationale context. Het valt op dat voor die term kennelijk slechts indirecte definities bestaan, althans ik heb

  9. Oral vaccination of fish

    NARCIS (Netherlands)

    Embregts, Carmen W.E.; Forlenza, Maria

    2016-01-01

    The limited number of oral vaccines currently approved for use in humans and veterinary species clearly illustrates that development of efficacious and safe oral vaccines has been a challenge not only for fish immunologists. The insufficient efficacy of oral vaccines is partly due to antigen

  10. Access and Fishing Activities

    DEFF Research Database (Denmark)

    Høst, Jeppe Engset

    2015-01-01

    a detailed ethnographic description of five different fishing operations and then compare them on a number of different fronts. This will direct us to some general differences in their modes of operation in relation to the vessel quota share (VQS) system and lead us to the next chapter, where the principal...

  11. Fish and shellfish allergy.

    Science.gov (United States)

    Thalayasingam, Meera; Lee, Bee-Wah

    2015-01-01

    Fish and shellfish consumption has increased worldwide, and there are increasing reports of adverse reactions to fish and shellfish, with an approximate prevalence of 0.5-5%. Fish allergy often develops early in life, whilst shellfish allergy tends to develop later, from adolescence onwards. Little is known about the natural history of these allergies, but both are thought to be persistent. The clinical manifestations of shellfish allergy, in particular, may vary from local to life-threatening 'anaphylactic' reactions within an individual and between individuals. Parvalbumin and tropomyosin are the two major allergens, but several other allergens have been cloned and described. These allergens are highly heat and biochemically stable, and this may in part explain the persistence of these allergies. Diagnosis requires a thorough history, skin prick and in-vitro-specific IgE tests, and oral challenges may be needed for diagnostic confirmation. Strict avoidance of these allergens is the current standard of clinical care for allergic patients, and when indicated, an anaphylactic plan with an adrenaline auto-injector is prescribed. There are no published clinical trials evaluating specific oral immunotherapy for fish or shellfish allergy. © 2015 S. Karger AG, Basel.

  12. Profil Gangguan Kognitif pada Tumor Intrakranial Primer dan Metastasis

    OpenAIRE

    Kartika Maharani; Andira Larasari; Tiara Aninditha; Yetty Ramli

    2015-01-01

    Gangguan kognitif sering menyertai pasien tumor intrakranial dan menjadi penyebab utama disabilitas. Perbedaan patofisiologi tumor intrakranial primer (TIP) dan metastasis (TM) menyebabkan perbedaan gambaran klinis dan derajat  gangguan kognitif. Tujuan penelitian ini untuk mengetahui prevalensi dan profil gangguan kognitif pasien TIP dan TM. Disain penelitian potong-lintang retrospektif menggunakan data sekunder dari Poliklinik Saraf RSCM pada bulan Januari 2011-Desember 2013. Subjek b...

  13. Isolasi, Karakterisasi dan Potensi Bakteri Aerob sebagai Pendegradasi Limbah Organik

    OpenAIRE

    Zahidah, Dinda; Shovitri, Maya

    2013-01-01

    Penelitian ini dilakukan untuk mengisolasi dan mengkarakterisasi bakteri aerob yang mampu mendegradasi amilum, protein dan selulosa. Penelitian ini berhasil memurnikan dan mengkarakterisasi isolat bakteri C5 yang cenderung masuk ke genus Bacillus. Berdasarkan uji kualitatif amilolitik, selulolitik dan proteolitik, diketahui bahwa isolat C5 memiliki indeks amilolitik (IA) sebesar 0.93, indeks selulolitik (IS) sebesar 1.95 dan indeks proteolitik (IP) sebesar 1.39

  14. Fish welfare in capture fisheries

    NARCIS (Netherlands)

    Veldhuizen, L.J.L.; Berentsen, P.B.M.; Boer, de I.J.M.; Vis, van de J.W.; Bokkers, E.A.M.

    2018-01-01

    Concerns about the welfare of production animals have extended from farm animals to fish, but an overview of the impact of especially capture fisheries on fish welfare is lacking. This review provides a synthesis of 85 articles, which demonstrates that research interest in fish welfare in capture

  15. BIOTECHNOLOGY OF THE FISH AQUACULTURE

    Directory of Open Access Journals (Sweden)

    L. P. Buchatsky

    2013-12-01

    Full Text Available The latest progress in biotechnology on fish aquaculture and different modern methods of investigations for increasing of fish productivity in aquaculture are analyzed. Except for the applied aspect, the use of modern biotechnological methods of investigations opens new possibilities for fundamental researches of sex-determining mechanisms, polyploidy, distant hybridization, and developmental biology of bony fishes. Review contains examples of utilizing modern biotechnology methods to obtain transgenic fishes with accelerated growth and for designing surrogate fishes. Methods for receiving unisexual shoals of salmon and sturgeon female fishes with the view of obtaining a large quantity of caviar, as well as receiving sterile (triploid fishes are analyzed. Great attention is given to androgenesis, particularly to disperm one, in connection with the problem of conserving rare and vanishing fish species using only sperm genetic material. Examples how distant hybrids may be obtained with the use of disperm androgenesis and alkylated DNA are given. Methods of obtaining fish primordium germ cells, recent developments in cultivation of fish stem cells and their use in biotechnology, as well as ones of transplantation of oogonium and spermatogonium to obtain surrogate fishes. The examples of successful experiments on spermatogonial xenotransplantation and characteristic of antifreezing fish proteins and also the prospect of their practical usage are given.

  16. Time-resolved fluorescence spectroscopy

    International Nuclear Information System (INIS)

    Gustavsson, Thomas; Mialocq, Jean-Claude

    2007-01-01

    This article addresses the evolution in time of light emitted by a molecular system after a brief photo-excitation. The authors first describe fluorescence from a photo-physical point of view and discuss the characterization of the excited state. Then, they explain some basic notions related to fluorescence characterization (lifetime and decays, quantum efficiency, so on). They present the different experimental methods and techniques currently used to study time-resolved fluorescence. They discuss basic notions of time resolution and spectral reconstruction. They briefly present some conventional methods: intensified Ccd cameras, photo-multipliers and photodiodes associated with a fast oscilloscope, and phase modulation. Other methods and techniques are more precisely presented: time-correlated single photon counting (principle, examples, and fluorescence lifetime imagery), streak camera (principle, examples), and optical methods like the Kerr optical effect (principle and examples) and fluorescence up-conversion (principle and theoretical considerations, examples of application)

  17. RESPON DAN KOPING PASIEN DM POST AMPUTASI

    Directory of Open Access Journals (Sweden)

    Candra Kusuma Negara

    2017-09-01

    Full Text Available Penyakit DM merupakan masalah kesehatan yang sangat penting karena berkaitan dengan tingginya kejadian komplikasi dan mortalitas yang tinggi. Bagi kebanyakan orang penyakit DM adalah suatu penyakit yang sangat mengkhawatirkan dan masyarakat sadar akan besarnya potensi bahaya yang ditimbulkannya. Bagi individu yang menderita DM dengan pasca amputasi, kehidupan selanjutnya merupakan babak baru yang penuh tantangan dan perubahan serta akan melalui proses koping terhadap proses perubahan tersebut. Secara umum penelitian ini bertujuan untuk mengeksplorasi berbagai pengalaman pasien DM pasca amputasi tentang respon dan koping yang dialaminya. Penelitian ini menggunakan studi fenomenologi. Pengambilan data menggunakan indepth interview pada empat orang partisipan yang dirawat jalan di Poli kaki diabetic RSUD Ulin Banjarmasin yang dilengkapi dengan pedoman wawancara dan informed consent. Metode analisis yang terstruktur dari Creswell menjadi 6 langkah. Terdapat 2 Tema yang ditemukan dalam penelitian ini yaitu berbagai respon post amputasi dan Berbagai koping pasien DM post amputasi. Berbagai respon post amputasi terdiri dari tiga Sub-Tema yaitu: (1 Mengalami hambatan fisik, (2 Mengalami perubahan peran, (3 Mengalami proses berduka, dan Terdapat empat Sub-Tema yang menggambarkan berbagai koping pasien DM post amputasi yaitu: (1 Lebih banyak beribadah, (2 Menerima keadaan, (3 Motivasi yang kuat, (4 Mencari dukungan sosial.

  18. Pengaruh Lama Perendaman dan Jenis Minuman Beralkohol Bir dan Tuak terhadap Kekerasan Email Gigi Manusia (In Vitro)

    OpenAIRE

    Magista, Malida; Nuryanti, Archadian; Wahyudi, Ivan Arie

    2014-01-01

    Erosi gigi merupakan hilangnya lapisan email gigi karena asam. Jenis asam, pH rendah, serta kandungan kalsium, fosfat, dan fluoride pada bir dan tuak diduga merupakan faktor kimiawi penyebab erosi gigi. Tujuan penelitian ini untuk mengetahui pengaruh lama perendaman dan jenis minuman beralkohol bir dan tuak terhadap kekerasan email gigi manusia (in vitro). Penelitian ini menggunakan 14 sampel gigi premolar pertama atas. Setiap gigi dibagi menjadi 2 bagian, bukal dan palatal. kemudian dibagi m...

  19. Pengaruh Lama Perendaman Dan Jenis Minuman Beralkohol Bir Dan Tuak Terhadap Kekerasan Email Gigi Manusia (in Vitro)

    OpenAIRE

    Magista, Malida; Nuryanti, Archadian; Wahyudi, Ivan Arie

    2014-01-01

    Erosi gigi merupakan hilangnya lapisan email gigi karena asam. Jenis asam, pH rendah, serta kandungan kalsium, fosfat, dan fluoride pada bir dan tuak diduga merupakan faktor kimiawi penyebab erosi gigi. Tujuan penelitian ini untuk mengetahui pengaruh lama perendaman dan jenis minuman beralkohol bir dan tuak terhadap kekerasan email gigi manusia (in vitro). Penelitian ini menggunakan 14 sampel gigi premolar pertama atas. Setiap gigi dibagi menjadi 2 bagian, bukal dan palatal. kemudian dibagi m...

  20. Pengaruh Perbedaan Jenis Asam dan Waktu Demineralisasi pada Nilai Rendemen dan Sifat Fisiko Kimia Gelatin Tulang Sapi Bali.

    OpenAIRE

    Ramadani, Dewi

    2014-01-01

    2014 Dewi Ramadani (I 411 10 267). Pengaruh Perbedaan Jenis Asam dan Waktu Demineralisasi pada Nilai Rendemen dan Sifat Fisiko Kimia Gelatin Tulang Sapi Bali. Muhammad Irfan Said Sebagai Pembimbing Utama dan Farida Nur Yuliati Sebagai Pembimbing Anggota. Gelatin merupakan senyawa hasil hidrolisis serabut kolagen secara persial. Tujuan dari penelitian ini adalah untuk melihat pengaruh jenis larutan dan waktu demineralisasi pada nilai rendemen dan sif...

  1. Single Molecule Spectroscopy of Fluorescent Proteins

    NARCIS (Netherlands)

    Blum, Christian; Subramaniam, Vinod

    2009-01-01

    The discovery and use of fluorescent proteins has revolutionized cellular biology. Despite the widespread use of visible fluorescent proteins as reporters and sensors in cellular environments the versatile photophysics of fluorescent proteins is still subject to intense research. Understanding the

  2. Fluorescent multiplex cell flow systems and methods

    KAUST Repository

    Merzaban, Jasmeen; Abuelela, Ayman F.; Mohammad, Amal Jehad

    2017-01-01

    scanning system emits multiple electromagnetic wavelengths simultaneously it cause multiple fluorescent labels having different excitation wavelength maximums to fluoresce. The system can simultaneously capture real-time fluorescence images from at least

  3. Epistaksis dan Hipertensi : Adakah Hubungannya?

    Directory of Open Access Journals (Sweden)

    Bestari J. Budiman

    2012-09-01

    Full Text Available Abstrak Latar Belakang: Epistaksis merupakan suatu kondisi klinis yang sering ditemui dan dapat terjadi pada semua umur dengan banyak variasi penyebabnya. Salah satu faktor risiko yang diduga ikut berperan dalam terjadinya epistaksis adalah hipertensi. Tujuan: Menjelaskan hubungan antara epistaksis dengan hipertensi. Tinjauan Pustaka: Hipertensi diduga tidak menyebabkan epistaksis secara langsung, tapi memperberat episode epistaksis. Mengendalikan tekanan darah sebagai salah satu faktor risiko, akan menurunkan insiden terjadinya epistaksis. Di ruang gawat darurat, pemberian obat anti hipertensi diberikan sebelum atau bersamaan dengan manajemen epistaksis itu sendiri. Kesimpulan: Terdapat hubungan antara epistaksis dengan hipertensi yang berlangsung lama dan adanya hipertrofi ventrikel kiri. Kata kunci : Hipertensi, kegawatdaruratan, penatalaksanaan epistaksis. Abstract Background: Epistaxis is a common clinical problem in all age groups with varied etiological factors. Hypertension has been suggested as a risk factor in epistaxis case. Purpose: To explain relationship between epistaxis and hypertension. Review: It has been suggested that hypertension does not cause epistaxis directly, but hypertension prolongs the episode of epistaxis when it does occur. The controlling for blood pressure as a risk factor will be decreased the incidencies of epistaxis. In emergency rooms, high blood pressure is usually treated before or in parallel with the management of epistaxis. Conclusion: There was an association between epistaxis and long duration of hypertension in adult and left ventricle hypertrophy. Key words : Hypertension, emergency case, management of epistaxis.

  4. Perbedaan konsumsi buah dan sayur pada anak sekolah dasar yang obes dan tidak obes di Kota Yogyakarta dan Kabupaten Bantul

    Directory of Open Access Journals (Sweden)

    Irma Nuraeni

    2016-03-01

    gender and energy intake, showed that obese  children who rarely consuming fruits (<7 times/week (OR=2,24, 95%CI: 1.53-3.28, rarely consuming vegetables (<7 times/week (OR=2,52, 95%CI: 1,70-3,73, and consuming fruits and vegetables less than 5 servings/day (equivalent to 400 g/day (OR= 4,59, 95%CI:2,11-10,00 were greater risk for being obesity.Conclusion:Obese children had rarely and less consume of fruits and vegetables than that did in non-obese children at Yogyakarta Municipality and District of Bantul. The children rarely and less consuming fruits and vegetables increased the risk of obesity.KEYWORDS: children obesity, vegetable, fruitABSTRAKLatar Belakang: Indonesia saat ini mengalami masalah gizi ganda, yaitu masalah gizi kurang dan gizi lebih. Kelebihan  gizi atau obesitas pada anak dan remaja apabila tidak diatasi maka berdampak menjadi obesitas pada masa dewasa yang berpotensi mengalami penyakit tidak menular, seperti jantung, hipertensi dan diabetes mellitus. Prevalensi obesitas pada anak di Provinsi Daerah Istimewa Yogyakarta mengalami peningkatan dari tahun ke tahun (1; 2. Obesitas disebabkan ketidakseimbangan antara masukan dengan keluaran energi. Anak cenderung mengkonsumsi padat energi yang berasa manis dan berlemak tinggi serta makanan kurang serat dari buah dan sayur. Beberapa penelitian menunjukkan peningkatan risiko obesitas pada orang yang kurang konsumsi buah dan sayur.Tujuan: Untuk mengetahui perbedaan frekuensi dan jumlah konsumsi buah dan sayur pada anak SD obes dan tidak obes di Kota Yogyakarta dan Kabupaten Bantul serta peran konsumsi buah dan sayur terhadap kejadian obesitas. Metode: Rancangan penelitian ini adalah case-control, 244 kasus (anak obes dan 244 kontrol (anak tidak obes. Subjek penelitian adalah anak usia 6-12 tahun yang duduk di kelas 1 hingga kelas 5 sekolah dasar di Kota Yogyakarta dan di Kabupaten Bantul. Data identitas diperoleh dari kuesioner terstruktur, sedangkan data frekuensi dan jumlah konsumsi buah dan sayur diperoleh

  5. ANEMIA DAN LAMA KONSUMSI OBAT ANTI TUBERCULOSIS

    Directory of Open Access Journals (Sweden)

    Anny Thuraidah

    2017-12-01

    Full Text Available Tuberkulosis adalah penyakit infeksi akibat kuman Mycobacterium tuberculosis yang dapat menginfeksi beberapa organ tubuh, diantaranya paru-paru, ginjal, dan tulang.Tujuan pengobatan tuberkulosis adalah memusnahkan basil tuberkulosis dengan cepat dan mencegah kekambuhan. Obat anti tuberkulosis (OAT dapat diterima dalam terapi, namun mempunyai efek toksik seperti agranulositosis, eosinofilia, trombositopenia dan anemia. Penelitian ini bertujuan untuk mengetahui hubungan lama konsumsi OAT pada pasien TB paru terhadap anemia. Penelitian ini bersifat Survey Analitic dengan rancangan Cross Sectional. Hasil penelitian rata-rata hitung sel darah merah 0 bulan 5,16 106/uL, 2 bulan 4,39 106/uL dan 6 bulan 4,61 106/uL, rata-rata kadar hemoglobin 0 bulan 15,17 g/dL, 2 bulan 12,73 g/dL dan 6 bulan 13,28 g/dL serta ratarata nilai hematokrit 0 bulan 44,26 %, 2 bulan 38,24 % dan 6 bulan 39,04 %. Dari hasil uji Spearman diperoleh nilai signifikan sebesar hitung sel darah merah 0,004 < α (0,05, kadar hemoglobin 0,007 < α (0,05 dan nilai hematokrit 0,015 < α (0,05 sehingga dapat disimpulkan ada hubungan antara lama konsumsi obat anti tuberkulosis pada pasien TB paru terhadap anemia dan nilai correlation coefficient hitung sel darah merah -0,531, kadar hemoglobin -0,479 serta nilai hematokrit -0,440 berarti memiliki kekuatan hubungan yang sedang. Disarankan untuk penelitian selanjutnya menggunakan parameter yang berbeda seperti jumlah trombosit, kadar AST/ALT dan sebaiknya menggunakan sampel atau pasien yang sama dari 0 bulan sampai 6 bulan.

  6. FISH as A method for detection of radiation Induced genetic damage

    International Nuclear Information System (INIS)

    Lakatosova, M.; Holeckova, B.

    2006-01-01

    Fluorescence in situ hybridization (FISH) has been considered as a suitable method for rapid and easy detection of chromosome aberrations. In contrast to the standard conventional staining procedure, this technique enables the detection and specification of stable chromosomal re-arrangements, which are compatible with cellular division and thus, they could be transmitted from common ancestral to next cell generations. FISH chromosome - specific painting probes have been effectively applied for the detection of chromosomal damage after exposure to radiation. During last years, several specific fluorescent labeled probes were performed that allowed precise detection of centromeres, sub-telomeres or other regions (sequences) in genome. Our paper deals with describing of different types of FISH probes and their possibilities for application in radiobiology. (authors)

  7. ISOLASI DAN IDENTIFIKASI KOMPONEN KIMIA MINYAK ATSIRI DAUN LEGUNDI (Vitex trifolia L.) DAN UJI AKTIVITAS ANTIMIKROBANYA

    OpenAIRE

    Mufidah, S.Si., M.Si., Apt.

    2004-01-01

    Dalam rangka pengembangan obat tradisional, telah dilakukan isolasi dan uji aktivitas antimikroba minyak atsiri daun legundi (Vitex trifolia, L.) familia Verbenaceae. Penelitian ini bertujuan untuk mengisolasi, menganalisis dan menguji aktivitas antimikroba minyak atsiri daun legundi. Isolasi dilakan dengan alat destilasi Stahl, diperoleh minyak atsiri sebanyak 0,02% v/b daun segar dan 0,08% v/b daun kering. Dari interpretasi data GC-MS tampak adanya 1,8 ???Cineole (17,83%), ???-terpiny...

  8. Status Oral Higiene Dan Periodontal Pada Pasien Diabetes Melitus Dan Non- Diabetes Di RSUD Dr. Pirngadi

    OpenAIRE

    Butar-Butar, Mardiah Rizqo

    2014-01-01

    Diabetes melitus adalah penyakit gangguan metabolisme ditandai dengan meningkatnya kadar gula darah yang terjadi karena kelainan sekresi insulin, kerja insulin, atau kedua-duanya. Diabetes melitus merupakan salah satu penyakit sistemik yang berperan sebagai faktor risiko penyakit periodontal. Penelitian ini bertujuan untuk mengetahui status oral higiene dan periodontal dan odd rasio periodontitis pada pasien Diabetes melitus dan non-Diabetes. Rancangan penelitian adalah case-control. Popul...

  9. Penskalaan Butir Format Respons Pilihan dan Respons Bebas Berdasarkan Model Rasch dan Partial Credit

    Directory of Open Access Journals (Sweden)

    Eko Hariadi

    2007-06-01

    Full Text Available Penelitian melihat pengaruh jumlah parameter butir, kategori respons bebas (RB, pengaruh sampel terhadap akurasi estimasi parameter kemampuan untuk menghasilkan estimasi yang stabil dan pengaruh pembobotan butir RP dan butir RB terhadap kesalahan baku. Penelitian dalam dua tahap, simulasi menggunakan 30 kondisi dengan replikasi 50 dengan variabel panjang tes, jumlah kategori, dan jumlah parameter butir, dan analisis deskriptif, dilanjutkan penerapan penskalaan gabungan butir tipe respons pilihan (rp dan butir respons bebas (rb pada konstruksi tes elektronika yang terdiri 40 butir pilihan ganda dan 4 butir jawaban tersusun, 3 butir memiliki lima kategori jawaban dan 1 butir dengan 4 kategori jawaban, melibatkan 355 siswa. Hasil penelitian menunjukkan: ukuran sampel kurang berpengaruh pada root mean square error atau (RSME> dan korelasi antara 9 dengan 0, namun berpengaruh terhadap akurasi estimasi parameter butir pilihan ganda (/>/y,dan parameter butir respons tersusun (3^- Jumlah parameter butir berpengaruh terhadap parameter kemampuan, tetapi tidak berpengaruh terhadap akurasi dari b^, dan S„,. Estimasi dari parameter tingkat kesulitan butir jawaban tersusun tiga kategori lebih akurat daripada butir jawaban tersusun lima kategori. Estimasi tahan {robust untuk parameter kesulitan butir jawaban tersusun 5 kategori memerlukan sampel minimal 250 responden, sedangkan untuk butir respons tersusun 3 kategori memerlukan sampel minimal 100 responden. Estimasi parameter kemampuan dari skor total (0^^ tidak sama dengan rata-rata jumlah tbeta dari masing-masing subtes (0^ + 0CR. Theta dari tes yang dikalibrasi bersama-sama berbeda dengan theta dari total subtes yang dikalibrasi secara terpisah. Korelasi kemampuan yang mengunakan pembobotan dan kemampuan tanpa pembobotan mempunyai suatu rentang dari 0,988 sampai 0,948. Kata kunci: penyekaiaan, model rash dan partial credit.

  10. Prinsip Transparansi dalam Perjanjian TBT dan SPS

    Directory of Open Access Journals (Sweden)

    Dina Widyaputri Kariodimedjo

    2012-02-01

    Full Text Available Transparency principle in the TBT and SPS Agreement is implemented by notification. Its application is prepared by related agencies through the making of regulations, establishment of enquiry points, building capacity and infrastructure. It guarantees certainty, predictability and credibility of Indonesia in international trade, and its application should not undermine national trade policy. Prinsip transparansi dalam Perjanjian TBT dan SPS diterapkan melalui notifikasi. Penerapan prinsip ini dilakukan oleh instansi-instansi terkait melalui pembuatan peraturan, upaya terkait kelembagaan, SDM dan infrastruktur. Prinsip yang bertujuan menciptakan kepastian, konsistensi bagi perdagangan dan kredibilitas Indonesia dalam perdagangan internasional, harus dilakukan tanpa mengorbankan kepentingan untuk mengamankan kebijakan impor nasional.

  11. SPIRITUALITAS MURIA: AKOMODASI TRADISI DAN WISATA

    Directory of Open Access Journals (Sweden)

    Ahmad Falah

    2012-12-01

    Tradisi “nyekar” sangat dikenal oleh orang Jawa, khususnya di makam Muria Kudus Jawa Tengah. Penelitian ini mengungkap bahwa kebanyakan peziarah makam ini bertujuan untuk mendapatkan keberkahan (tabarrukan, keamanan dan kemudahan dalam hidup, menguasai ilmu linuwih, disembuhkan dari penyakit, dan mengharapkan kebaikan dari nenek moyang. Kunjungan mereka ke makam itu memberikan dampak yang besar bagi kehidupan masyarakat, baik secara ekonomi maupun sosial. Dalam perspektif ekonomi, lebih dari 1.500 tenaga kerja tergantung pada keberadaan makam Muria. Dampak yang lain adalah slidaritas setempat, kompetisi sosial, dan kesempatan kerja yang mengakibatkan munculnya tumbuhnya pragmatisme di kalangan anggota masyarakat Muria.

  12. Media Sosial dan Perkembangan Komunikasi Korporat

    Directory of Open Access Journals (Sweden)

    Rina Juwita

    2017-07-01

    Full Text Available Kehadiran media sosial telah mengubah aktivitas komunikasi korporat dan dengan cepat merevolusi bagaimana kampanye atau program public relations berjalan. Jika dibandingkan dengan cara tradisional yang mengandalkan pada output murni, media sosial memaksa komunikasi korporat beralih pada proses dialog dimana para pemangku kepentingan, dan bukan hanya perusahaan sama-sama memiliki kekuasaan terhadap pesan yang beredar. Media sosial merupakan alat komunikasi revolusioner yang mengubah secara cepat bagaimana praktik PR menjadi bagian integral dari komunikasi korporat bagi sejumlah perusahaan dan menawarkan pilihan baru bagi para praktisi PR pada setiap aspek proses komunikasi yang dilakukan.

  13. Inovasi Desain, Teknologi, dan Pemasaran Lewat Website Usaha Kecil Menengah Batik dan Lutik (Lurik Batik di Kecamatan Laweyan Surakarta

    Directory of Open Access Journals (Sweden)

    Anastasia Riani Suprapti

    2016-12-01

      Tujuan penelitian terapan ini adalah untuk meningkatkan produktivitas dan mengembangkan produk batik dan lutik yang ada di wilayah Kecamatan Laweyan Surakarta dengan inovasi desain dan teknologi baru. Program ini bekerja sama dengan mitra UKM Batik Dewi (UKM 1 dan Batik Sinung Rejeki (UKM2 yang terletak di Kecamatan Laweyan. Pengusaha batik Laweyan dengan produknya berupa batik dan tekstil bermotif batik (printing dan cap dalam era globalisasi sekarang ini ternyata memiliki daya tahan yang baik, ditengah persaingan dengan produk sejenis dari daerah lain maupun dari luar negeri. Secara garis besar pola yang digunakan dalam implementasi penelitian ini meliputi: diskusi (FGD, kerja bengkel, operasional pabrik, pelatihan dan pendampingan (kelompok dan individu. Seperti halnya Usaha Kecil Menengah pada umumnya, pengusaha batik di Laweyan juga menghadapi masalah Internal meliputi  permodalan, inovasi desain, pemasaran, dan manajemen keuangan, yang meliputi administrasi keuangan/pembukuan. Beberapa solusi yang ditawarkan untuk mengatasi permasalahan yang dihadapi diantaranya adalah melakukan Inovasi desain dan teknologi, pengaturan layout pabrik, dan pemasaran lewat web. Selain itu mengembangkan desain motif batik yang lebih diminati pasar diberikan juga untuk pengembangan teknik kombinasi batik-lurik ikat dengan cara lukis dan pengelantangan. Penggunaan media teknologi informasi dan komunikasi untuk promosi lewat website. Adapun untuk usaha pengembangan manajemen pemasaran,  diusahakan media promosi dan menjalin kerjasama pemasaran  dengan instansi terkait, misalnya dengan mengikuti pameran-pameran di Solo dan Jakarta.

  14. Marine Fish Hybridization

    KAUST Repository

    He, Song

    2017-04-01

    Natural hybridization is reproduction (without artificial influence) between two or more species/populations which are distinguishable from each other by heritable characters. Natural hybridizations among marine fishes were highly underappreciated due to limited research effort; it seems that this phenomenon occurs more often than is commonly recognized. As hybridization plays an important role in biodiversity processes in the marine environment, detecting hybridization events and investigating hybridization is important to understand and protect biodiversity. The first chapter sets the framework for this disseration study. The Cohesion Species Concept was selected as the working definition of a species for this study as it can handle marine fish hybridization events. The concept does not require restrictive species boundaries. A general history and background of natural hybridization in marine fishes is reviewed during in chapter as well. Four marine fish hybridization cases were examed and documented in Chapters 2 to 5. In each case study, at least one diagnostic nuclear marker, screened from among ~14 candidate markers, was found to discriminate the putative hybridizing parent species. To further investigate genetic evidence to support the hybrid status for each hybrid offspring in each case, haploweb analysis on diagnostic markers (nuclear and/or mitochondrial) and the DAPC/PCA analysis on microsatellite data were used. By combining the genetic evidences, morphological traits, and ecological observations together, the potential reasons that triggered each hybridization events and the potential genetic/ecology effects could be discussed. In the last chapter, sequences from 82 pairs of hybridizing parents species (for which COI barcoding sequences were available either on GenBank or in our lab) were collected. By comparing the COI fragment p-distance between each hybridizing parent species, some general questions about marine fish hybridization were discussed: Is

  15. KANDUNGAN ASAM LEMAK DAN KARAKTERISTIK FISIKO-KIMIA MINYAK IKAN LELE DAN MINYAK IKAN LELE TERFERMENTASI (FATTY ACID CONTENTS AND PHYSICO-CHEMICAL CHARACTERISTICS OF CATFISH OIL AND FERMENTED CATFISH OIL

    Directory of Open Access Journals (Sweden)

    Iskari Ngadiarti

    2013-06-01

    Full Text Available ABSTRACT Catfish oil and fermented catfish oil have not been developed and commercially produced as catfish derived products. Various processing of catfish oil will change both the composition and physico-chemical characteristics. The objective of this study was to identify the composition of fatty acids and physico-chemical characteristics of both catfish oil (MIL and fermented catfish oil (MILT. Results showed that fatty acid composition of catfish oil was MUFA (36.12%> PUFA> (32.43%> SFA (31.45%, while the composition of fermented catfish oil was MUFA (42.96%> SFA (42.32%> PUFA (15.39%. The fermentation process with lactic acid bacteria increased the content of stearic acid but decreased the content of linoleic acid and linolenic acid. Physical and chemical characteristics of MIL and MILT were almost similar, they indicated oxidation process, based on melting point, viscosity, and thiobarbituric acid values. Therefore, in the production of catfish oil or fermented cat fish oil, it is necessary to add spices and/or nutrient as antioxidants sources. Keywords: catfish oil, PUFA, MUFA, SFA and CLA  ABSTRAK Minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT sebagai produk turunan dari ikan lele masih belum banyak dikembangkan dan diproduksi secara komersial. Berbagai proses pengolahan minyak dapat mengubah komposisi dan karakteristik fisiko-kimia minyak ikan lele. Tujuan dari penelitian ini adalah untuk mengidentifikasi komposisi asam lemak dan karakteristik fisiko-kimia dari minyak ikan lele (MIL dan minyak ikan lele terfermentasi (MILT. Komposisi asam lemak pada MIL secara berurutan adalah MUFA (36,12% > PUFA (32,43% > SFA (31,45%, sedangkan setelah difermentasi menjadi MILT terjadi perubahan yaitu MUFA (42,96% > SFA (42,32% > PUFA (15,39%. Jenis asam lemak jenuh yang mengalami peningkatan  pada MILT adalah asam lemak stearat, sedangkan asam lemak tidak jenuh ganda yang mengalami penurunan adalah asam linoleat dan linolenat yang

  16. Pengembangan Sistem Informasi Akuntansi Dan Pengelolaan Keuangan Modul Bendahara Menggunakan Teknologi Java dan PostgreSQL

    Directory of Open Access Journals (Sweden)

    Alfie Satria Hidayat

    2012-09-01

    Full Text Available Penerapan sistem informasi saat ini sudah menjadi kebutuhan yang krusial bagi proses bisnis instansi pemerintah. Direktorat Jenderal Perbendaharaan adalah salah satu instansi pemerintah di bawah Kementerian Keuangan yang memiliki tugas pokok dan fungsi terkait dengan siklus anggaran (budget cycle mulai dari perencanaan, pelaksanaan, sampai pertanggungjawaban anggaran. Saat ini terdapat banyak aplikasi yang dikembangkan oleh Direktorat Jenderal Perbendaharaan untuk membantu satuan kerja sebagai mitra kerja dalam pengelolaan Anggaran Pendapatan dan Belanja Negara. Namun, aplikasi-aplikasi tersebut belum terintegrasi padahal dalam proses bisnisnya semuanya saling terkait dan berurutan. Selain itu, beberapa aplikasi menggunakan database yang sama sehingga dimungkinkan terjadi duplikasi data dan memerlukan sinkronisasi jika terjadi perubahan. Diantara aplikasi yang ada, salah satunya terdapat aplikasi pembukuan bendahara. Melalui penelitian ini, penulis akan membuat Sistem Informasi Akuntansi dan Pengelolaan Keuangan (SIAPKAN  khususnya modul bendahara sebagai solusi untuk menjawab permasalahan dengan mengintegrasikan semua aplikasi yang terkait dalam proses siklus anggaran. Aplikasi ini merupakan aplikasi berbasis web yang akan dikembangkan dengan menggunakan bahasa pemrograman Java (Servlet dan JSP. Aplikasi ini akan menggunakan single database yaitu dengan database PostgreSQL sehingga data yang diolah dan disajikan lebih akurat dan proses bisnis akan berjalan lebih efektif dan efisien.

  17. Isolasi dan Identifikasi Mikroba Lipolitik dari Limbah Cair Surimi dan Rajungan

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    Devi Ambarwati Oktavia

    2017-05-01

    Full Text Available Industri pengolahan hasil perikanan di sepanjang pantai Utara Jawa seperti pengalengan rajungan di Cirebon (Jawa Barat dan pengolahan surimi di Kendal (Jawa Tengah, menghasilkan air limbah yang mengandung banyak protein dan lemak. Bakteri yang hidup di limbah yang banyak mengandung protein dan lemak tersebut diperkirakan memiliki kemampuan untuk menghidrolisis lemak menjadi asam lemak dan gliserol sehingga berpotensi untuk dimanfaatkan sebagai bioremedian alami bagi penanganan air limbah hasil perikanan di tempat lain. Penelitian ini bertujuan untuk melakukan penapisan dan identifikasi bakteri lipolitik potensial sebagai bioremedian air limbah perikanan. Penapisan dilakukan terhadap 11 isolat bakteri lipolitik dari air limbah yang diambil dari industri pengalengan rajungan di Cirebon dan pengolahan surimi di Kendal dengan menggunakan media spesifik agar tributirin. Isolat bakteri lipolitik potensial ditentukan berdasarkan zona bening yang terbentuk di sekitar koloni, yaitu sekurang-kurangnya 6 mm. Isolat bakteri potensial ini selanjutnya diidentifikasi secara molekuler berdasarkan analisis sekuen 16S-rDNA. Dari penapisan diperoleh empat isolat bakteri potensial, yaitu isolat SPB, SHj, SOr dan SKn. Identifikasi molekuler menunjukkan bahwa isolat SPB dan SHj masing-masing adalah Serratia fonticola 10AdanBacillus cereus strain 103.2.2dengan kemiripan 97%, isolat SOr memiliki kemiripan 96% dengan Bacillus pumilus strain vit bac1 dan isolat SKn adalah Enterococcus pseudoavium strain L3C21K2dengan kemiripan 87%. Keempat isolat tersebut berpotensi untuk dimanfaatkan sebagai bioremedian pada air limbah dari industri pengolahan hasil perikanan di Indonesia.

  18. EKSISTENSI DAN SEBARAN NYAMUK AEDES AEGYPTI DAN AEDES ALBOPICTUS DI KAMPUS UNIVERSITAS HASANUDDIN MAKASSAR

    Directory of Open Access Journals (Sweden)

    Yosefina Dota T

    2015-03-01

    Full Text Available Penelitian yang bertujuan untuk mengetahui eksistensi dan sebaran nyamuk Aedes aegypti dan Aedes albopictus telah dilakukan di Kampus Universitas Hasanuddin, Kec. Tamalanrea, Makassar. Penelitian bersifat eksploratif dengan melakukan sampling terhadap lima lokasi yaitu : a Fak. Peternakan (Utara, b Fak. Hukum (Timur, c Pusat Kegiatan Penelitian/PKP (Selatan, d Workshop/Dekat Pondokan mahasiswa (Barat dan e Fak. MIPA (Tengah. Sampling nyamuk menggunakan metode ovitrap (menggunakan attraktan Eluisine Indica L. dan survei terhadap berbagai tempat penampungan air. Sampel telur dan larva nyamuk yang diperoleh disimpan dalam microtube berisi alkohol 70% kemudian diidentifikasi berdasarkan Rueda (2004. Hasil penelitian menunjukkan bahwa Nyamuk Ae. aegypti dan Ae. albopictus ditemukan hidup dan berkembang biak di kampus Universitas Hasanuddin, Makassar. Eksistensi dan sebaran kedua jenis nyamuk tersebut dipengaruhi oleh faktor adanya manusia/masyarakat kampus yang beraktivitas baik di dalam ruangan (indoor maupun di luar ruangan (outdoor, adanya berbagai tempat penampungan air baik buatan (bak mandi, ember maupun barang bekas (botol/kaleng bekas, tempurung kelapa, vegetasi/tanaman dan berbagai macam hewan yang berada di sekitaran kampus. Hasil penelitian dalam ruangan (indoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di dalam ruangan gedung PKP sedangkan Ae.albopictus lebih banyak di Fak. Hukum. Hasil penelitian di luar ruangan (outdoor menunjukkan bahwa nyamuk Ae. aegypti lebih banyak ditemukan hidup di area Workshop sedangkan Ae.albopictus lebih banyak di area PKP.

  19. Improving fish survival through turbines

    International Nuclear Information System (INIS)

    Ferguson, J.W.

    1993-01-01

    Much of what is known about fish passage through hydroturbines has been developed by studying migratory species of fish passing through large Kaplan turbine units. A review of the literature on previous fish passage research presented in the accompanying story illustrates that studies have focused on determining mortality levels, rather than identifying the causal mechanism involved. There is a need for understanding how turbine designs could be altered to improve fish passage conditions, how to retrofit existing units, and how proposed hydro plant operational changes may affect fish survival. The US Army Corps of Engineers has developed a research program to define biologically based engineering criteria for improving fish passage conditions. Turbine designs incorporating these criteria can be evaluated for their effects on fish survival, engineering issues, costs, and power production. The research program has the following objectives: To gain a thorough knowledge of the mechanisms of fish mortality; To define the biological sensitivities of key fish species to these mechanisms of mortality; To develop new turbine design criteria to reduce fish mortality; To construct prototype turbine designs, and to test these designs for fish passage, hydro-mechanical operation, and power production; and To identify construction and power costs associated with new turbine designs

  20. The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation

    Directory of Open Access Journals (Sweden)

    Rescan Pierre-Yves

    2010-05-01

    Full Text Available Abstract Background Fish skeletal muscle growth involves the activation of a resident myogenic stem cell population, referred to as satellite cells, that can fuse with pre-existing muscle fibers or among themselves to generate a new fiber. In order to monitor the regulation of myogenic cell differentiation and fusion by various extrinsic factors, we generated transgenic trout (Oncorhynchus mykiss carrying a construct containing the green fluorescent protein reporter gene driven by a fast myosin light chain 2 (MlC2f promoter, and cultivated genetically modified myogenic cells derived from these fish. Results In transgenic trout, green fluorescence appeared in fast muscle fibers as early as the somitogenesis stage and persisted throughout life. Using an in vitro myogenesis system we observed that satellite cells isolated from the myotomal muscle of transgenic trout expressed GFP about 5 days post-plating as they started to fuse. GFP fluorescence persisted subsequently in myosatellite cell-derived myotubes. Using this in vitro myogenesis system, we showed that the rate of muscle cell differentiation was strongly dependent on temperature, one of the most important environmental factors in the muscle growth of poikilotherms. Conclusions We produced MLC2f-gfp transgenic trout that exhibited fluorescence in their fast muscle fibers. The culture of muscle cells extracted from these trout enabled the real-time monitoring of myogenic differentiation. This in vitro myogenesis system could have numerous applications in fish physiology to evaluate the myogenic activity of circulating growth factors, to test interfering RNA and to assess the myogenic potential of fish mesenchymal stem cells. In ecotoxicology, this system could be useful to assess the impact of environmental factors and marine pollutants on fish muscle growth.