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Sample records for fluctuating redox conditions

  1. Ediacaran Redox Fluctuations

    Science.gov (United States)

    Sahoo, S. K.; Jiang, G.; Planavsky, N. J.; Kendall, B.; Owens, J. D.; Anbar, A. D.; Lyons, T. W.

    2013-12-01

    Evidence for pervasive oxic conditions, and likely even deep ocean oxygenation has been documented at three intervals in the lower (ca. 632 Ma), middle (ca. 580 Ma) and upper (ca. 551 Ma) Ediacaran. The Doushantuo Formation in South China hosts large enrichments of redox-sensitive trace element (e.g., molybdenum, vanadium and uranium) in anoxic shales, which are indicative of a globally oxic ocean-atmosphere system. However, ocean redox conditions between these periods continue to be a topic of debate and remain elusive. We have found evidence for widespread anoxic conditions through much of the Ediacaran in the deep-water Wuhe section in South China. During most of the Ediacaran-early Cambrian in basinal sections is characterized by Fe speciation data and pyrite morphologies that indicate deposition under euxinic conditions with near-crustal enrichments of redox-sensitive element and positive pyrite-sulfur isotope values, which suggest low levels of marine sulfate and widespread euxinia. Our work reinforces an emerging view that the early Earth, including the Ediacaran, underwent numerous rises and falls in surface oxidation state, rather than a unidirectional rise as originally imagined. The Ediacaran ocean thus experienced repetitive expansion and contraction of marine chalcophilic trace-metal levels that may have had fundamental impact on the slow evolution of early animals and ecosystems. Further, this framework forces us to re-examine the relationship between Neoproterozoic oxygenation and metazoan diversification. Varying redox conditions through the Cryogenian and Ediacaran may help explain molecular clock and biomarker evidence for an early appearance and initial diversification of metazoans but with a delay in the appearance of most major metazoan crown groups until close to Ediacaran-Cambrian boundary.

  2. Carbon Mineralization Can Be Sustained or Even Stimulated under Fluctuating Redox Conditions in Tropical and Temperate Soils

    Science.gov (United States)

    Huang, W.; Hall, S. J.

    2017-12-01

    Soil carbon (C) mineralization is widely thought to be affected by O2 availability, and anaerobiosis represents a significant global mechanism of C stabilization. However, mineral-associated organic C (e.g. Fe-bound organic C) may be vulnerable to redox fluctuations due to release following Fe reduction, which could counteract protective effects of anaerobiosis. Many soils, including temperate Mollisols and tropical Oxisols, experience fluctuating redox conditions following moisture variations that could impact C cycling and stabilization. Here we incubated two soils with C4 leaf litter at different duration and frequencies of anaerobic periods for 128 days to investigate how redox fluctuations affect soil C mineralization. The treatments included static aerobic (control), and 2-, 4-, 8- and 12- day anaerobic followed by 4-day aerobic. We measured CO2, CH4, and their C isotope ratios. Longer durations of anaerobic conditions promoted greater Fe reduction and more DOC released. Notably, in both soils despite their large differences in composition, the production of CO2 and CH4 was stimulated under aerobic conditions following anaerobic conditions (relative to the control), which compensated for the decrease under anaerobic conditions. After 128 days, cumulative C mineralization in the control was similar between the Mollisol (9.7 mg C g-1) and the Oxisol (10.1 mg C g-1). The value in the Mollisol was significantly higher in the 12-day anaerobic treatment (11.2 mg C g-1) than the aerobic control and the 2-day anaerobic treatment (9.7 mg C g-1). In the Oxisol, cumulative C mineralization was not significantly affected by any of the fluctuating redox treatments relative to the control. Our findings challenge theory by showing that redox fluctuations can counteract the suppressive effects of O2 limitation on decomposition.

  3. Effects of a fluctuating water table : Column study on redox dynamics and fate of some organic pollutants

    NARCIS (Netherlands)

    Sinke, A.J.C.; Dury, O.; Zobrist, J.

    1998-01-01

    The development of the redox conditions has been studied in an initially aerobic column filled with quartz sand coated with ferrihydrite and subjected to a fluctuating water table. The purpose of this study was to evaluate the effect of water table fluctuations on the redox dynamics and the fate of

  4. Characterization of redox conditions in pollution plumes

    DEFF Research Database (Denmark)

    Christensen, Thomas Højlund; Bjerg, Poul Løgstrup; Banwart, Steven A.

    2000-01-01

    Evalution of redox conditions in groundwater pollution plumes is often a prerequisite for understanding the behviour of the pollutants in the plume and for selecting remediation approaches. Measuring of redox conditions in pollution plumes is, however, a fairly recent issue and yet relative few...

  5. Soil phosphorus redistribution among iron-bearing minerals under redox fluctuation

    Science.gov (United States)

    Lin, Y.; Bhattacharyya, A.; Campbell, A.; Nico, P. S.; Pett-Ridge, J.; Silver, W. L.

    2016-12-01

    Phosphorus (P) is a key limiting nutrient in tropical forests that governs primary production, litter decomposition, and soil respiration. A large proportion of P in these highly weathered soils is bound to short-range ordered or poorly crystalline iron (Fe) minerals. It is well-documented that these Fe minerals are redox-sensitive; however, little is known about how Fe-redox interactions affect soil P turnover. We evaluated the impacts of oxic/anoxic fluctuation on soil P fractions and reactive Fe species in a laboratory incubation experiment. Soils from a humid tropical forest were amended with plant biomass and incubated for up to 44 days under four redox regimes: static oxic, static anoxic, high frequency fluctuating (4-day oxic/4-day anoxic), and low frequency fluctuating (8-day oxic/4-day anoxic). We found that the static anoxic treatment induced a 10-fold increase in Fe(II) (extracted by hydrochloric acid) and a 1.5-fold increase in poorly crystalline Fe (extracted by ammonium oxalate), suggesting that anoxic conditions drastically increased Fe(III) reduction and the formation of amorphous Fe minerals. Static anoxic conditions also increased Fe-bound P (extracted by sodium hydroxide) and increased the oxalate-extractable P by up to 110% relative to static oxic conditions. In two fluctuating treatments, Fe(II) and oxalate-extractable Fe and P were all increased by short-term reduction events after 30 minutes, but fell back to their initial levels after 3 hours. These results suggest that reductive dissolution of Fe(III) minerals mobilized a significant amount of P; however, this P could be rapidly re-adsorbed. Furthermore, bioavailable P extracted by sodium bicarbonate solution was largely unaffected by redox regimes and was only increased by static anoxic conditions after 20 days. Overall, our data demonstrate that a significant amount of soil P may be liberated and re-adsorbed by Fe minerals during redox fluctuation. Even though bioavailable P appears to be

  6. Dissolution of UO2 in redox conditions

    International Nuclear Information System (INIS)

    Casas, I.; Pablo de, J.; Rovira, M.

    1998-01-01

    The performance assessment of the final disposal of the spent nuclear fuel in geological formations is strongly dependent on the spent fuel matrix dissolution. Unirradiated uranium (IV) dioxide has shown to be very useful for such purposes. The stability of UO 2 is very dependent on vault redox conditions. At reducing conditions, which are expected in deep groundwaters, the dissolution of the UO 2 -matrix can be explained in terms of solubility, while under oxidizing conditions, the UO 2 is thermodynamically unstable and the dissolution is kinetically controlled. In this report the parameters which affect the uranium solubility under reducing conditions, basically pH and redox potential are discussed. Under oxidizing conditions, UO 2 dissolution rate equations as a function of pH, carbonate concentration and oxidant concentration are reported. Dissolution experiments performed with spent fuel are also reviewed. The experimental equations presented in this work, have been used to model independent dissolution experiments performed with both unirradiated and irradiated UO 2 . (Author)

  7. Redox Conditions Among the Terrestrial Planets

    Science.gov (United States)

    Jones, J. H.

    2004-01-01

    Early solar system conditions should have been extremely reducing. The redox state of the early solar nebula was determined by the H2O/H2 of the gas, which is calculated (based on solar composition) to have been about IW-5. At high temperature under such conditions, ferrous iron would exist only as a trace element in silicates and the most common type of chondritic material should have been enstatite chondrites. The observation that E-chondrites form only a subset of the chondrite suite and that the terrestrial planets (Earth, Moon, Mars, Venus, 4 Vesta) contain ferrous and ferric iron as major and minor elements, respectively, implies that either most chondritic materials formed under conditions that were not solar or that early-formed metals oxidized at low temperature, producing FeO. For example, equilibrated ordinary chondrites (by definition, common chondritic materials), by their phase assemblage of olivine, orthopyroxene and metal, must fall not far from the QFI (Quartz-Fayalite-Iron) oxygen buffer. The QFI buffer is about IW-0.5 and, as we shall see, this fo2 is close to that inferred for many materials in the inner solar system.

  8. Measuring intracellular redox conditions using GFP-based sensors

    DEFF Research Database (Denmark)

    Björnberg, Olof; Ostergaard, Henrik; Winther, Jakob R

    2006-01-01

    Recent years have seen the development of methods for analyzing the redox conditions in specific compartments in living cells. These methods are based on genetically encoded sensors comprising variants of Green Fluorescent Protein in which vicinal cysteine residues have been introduced at solvent......-exposed positions. Several mutant forms have been identified in which formation of a disulfide bond between these cysteine residues results in changes of their fluorescence properties. The redox sensors have been characterized biochemically and found to behave differently, both spectroscopically and in terms...

  9. Biodegradation of NSO-compounds under different redox-conditions

    DEFF Research Database (Denmark)

    Dyreborg, S.; Arvin, E.; Broholm, K.

    1997-01-01

    Laboratory experiments were carried out to investigate the potential of groundwater microorganisms to degrade selected heterocyclic aromatic compounds containing nitrogen, sulphur, or oxygen (NSO-compounds) under four redox-conditions over a period of 846 days. Eight compounds (pyrrole, 1-methylp...... together with the long lag periods and the low degradation rates under aerobic conditions suggest that NSO-compounds might persist in groundwater at creosote-contaminated sites. (C) 1997 Elsevier Science B.V....

  10. Hydrodynamics: Fluctuating initial conditions and two-particle correlations

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, R.P.G.; Grassi, F. [Instituto de Fisica, Universidade de Sao Paulo (Brazil); Hama, Y., E-mail: hama@fma.if.usp.b [Instituto de Fisica, Universidade de Sao Paulo (Brazil); Qian, W.-L. [Instituto de Fisica, Universidade de Sao Paulo (Brazil)

    2011-03-15

    Event-by-event hydrodynamics (or hydrodynamics with fluctuating initial conditions) has been developed in the past few years. Here we discuss how it may help to understand the various structures observed in two-particle correlations.

  11. Competing for phosphors under changing redox conditions: biological versus geochemical sinks

    Science.gov (United States)

    Gross, A.; Pett-Ridge, J.; Silver, W. L.

    2016-12-01

    Competing for phosphorus under changing redox conditions: biological versus geochemical sinksAvner Gross1, Jennifer Pett-Ridge2 and Whendee L Silver1 University of California Berkeley, Department of Environmental Science, Policy, & Management, Berkeley, CA, USA. Lawrence Livermore National Laboratory, Physical and Life Science Directorate, Livermore, CA, USA. The cycling of phosphorous (P) in highly weathered, humid tropical forest soils is tightly regulated by P sorption dynamics to the surfaces of Fe(III) (hydr)oxides and root and microbial demands for P. Periods of anoxic soil conditions, which are common in humid environments, induce the reduction of Fe (III) to Fe (II) and may release sorbed P into the soil solution. The microbial demand for P is influenced by the C and nutrient composition of their available substrates. Therefore, we hypothesize that soil redox conditions and substrate quality and availability will control the partitioning of P between microbial biomass and the soil mineral phase. The aim of this study was to examine how fluctuations in soil redox conditions and changes in microbial P demand affect the fate of new P that enters the soil solution. To achieve this aim we conducted a series of soil incubation experiments using a wet tropical soil from Puerto Rico (where redox conditions and P availability naturally oscillate) with a single pulse of phosphate (PO4), altering both the microbial activity and redox conditions. To follow the fate the added P, the added phosphate was labeled with 18O. As the exchange of oxygen between phosphate and water only occurs during biological processes, P-18O labeling can be used as an indicator of microbial use. To quantify sizes of the microbial and mineral P pools we used traditional chemical extractions in the bulk scale. We used NanoSIMS isotopic imaging to map the distribution of P-16O and P-18O and co-localization with Fe minerals at the nano scale. Our results show that the amount of the added P fixed

  12. Dissipation of oxytetracycline in soils under different redox conditions

    International Nuclear Information System (INIS)

    Yang Jigeng; Ying Guangguo; Zhou Lijun; Liu Shan; Zhao Jianliang

    2009-01-01

    This study investigated the dissipation kinetics of oxytetracycline in soils under aerobic and anoxic conditions. Laboratory experiments showed that the dissipation of oxytetracycline in soil followed first-order reaction kinetics and its dissipation rates decreased with increasing concentration. Oxytetracycline dissipated faster in soil under aerobic conditions than under anoxic conditions. The half-lives for oxytetracycline in soil under aerobic conditions ranged between 29 and 56 days for non-sterile treatments and 99-120 days for sterile treatments, while under anoxic conditions the half-lives of oxytetracycline ranged between 43 and 62 days in the non-sterile soil and between 69 and 104 days in the sterile soil. This suggests microbes can degrade oxytetracycline in agricultural soil. Abiotic factors such as strong sorption onto soil components also played a role in the dissipation of oxytetracycline in soil. - Oxytetracycline dissipation in soils is influenced by redox conditions and soil properties.

  13. New lab scale approaches for quantification of redox conditions

    Science.gov (United States)

    Fernandez, P. M.; Dathe, A.; Nadeem, S.; Bakken, L. R.; Bloem, E.; French, H. K.; Binley, A. M.

    2013-12-01

    Degradation of organic chemicals in the unsaturated zone is a process highly relevant for developing remediation techniques for protecting groundwater. Degradation causes changes in chemical composition of the water phase and gas releases. These changes can potentially be mapped with electrical resistivity measurements in the bulk soil and gas measurements at the soil surface. The redox potential combined with the local geological conditions determines the composition of available electron acceptors as well as microbial degradation pathways and how the soil system is affected in the long term. After oxygen and nitrate are depleted, manganese and iron should be reduced. However, in experiments conducted in the unsaturated zone at Gardermoen airport, Norway, it was found that for the degradation of the de-icing agent propylene glycol (PG), manganese and iron were preferred over nitrate as electron acceptor. A key hypothesis for the work presented is that for a designated soil, the redox potential affects gas releases and soil solution composition profoundly. As the redox potential decreases, the reactants of the degradation change and therefore the composition of the soil-water system changes. These changes can be quantified dynamically by gas measurements and changes in electrical conductivity of the pore water and electrical resistivity of the bulk soil. Batch experiments were conducted to examine whether nitrate is a preferred electron acceptor over iron and manganese oxides as described in classical redox reaction theory. Gas releases during PG and glutamate degradation were measured in a sandy pristine soil with and without nitrate under anaerobic condition during two weeks of incubation. Chemical reactions were quantified with the modelling tool ORCHESTRA. We are currently investigating whether dynamical measurements of electrical conductivity and bulk resistivity are suited to trace which electron acceptors (nitrate, manganese or iron) are being reduced. First

  14. Organic chemical degradation by remote study of the redox conditions

    Science.gov (United States)

    Fernandez, P. M.; Revil, A.; Binley, A. M.; Bloem, E.; French, H. K.

    2014-12-01

    Monitoring the natural (and enhanced) degradation of organic contaminants is essential for managing groundwater quality in many parts of the world. Contaminated sites often have limited access, hence non-intrusive methods for studying redox processes, which drive the degradation of organic compounds, are required. One example is the degradation of de-icing chemicals (glycols and organic salts) released to the soil near airport runways during winter. This issue has been broadly studied at Oslo airport, Gardermoen, Norway using intrusive and non-intrusive methods. Here, we report on laboratory experiments that aim to study the potential of using a self-potential, DCresistivity, and time-domain induced polarization for geochemical characterization of the degradation of Propylene Glycol (PG). PG is completely miscible in water, does not adsorb to soil particles and does not contribute to the electrical conductivity of the soil water. When the contaminant is in the unsaturated zone near the water table, the oxygen is quickly consumed and the gas exchange with the surface is insufficient to ensure aerobic degradation, which is faster than anaerobic degradation. Since biodegradation of PG is highly oxygen demanding, anaerobic pockets can exist causing iron and manganese reduction. It is hypothesised that nitrate would boost the degradation rate under such conditions. In our experiment, we study PG degradation in a sand tank. We provide the system with an electron highway to bridge zones with different redox potential. This geo-battery system is characterized by self-potential, resistivity and induced polarization anomalies. An example of preliminary results with self-potential at two different times of the experiment can be seen in the illustration. These will be supplemented with more direct information on the redox chemistry: in-situ water sampling, pH, redox potential and electrical conductivity measurements. In parallel, a series of batch experiments have been

  15. Targeting the Redox Balance in Inflammatory Skin Conditions

    Directory of Open Access Journals (Sweden)

    Ditte M. S. Lundvig

    2013-04-01

    Full Text Available Reactive oxygen species (ROS can be both beneficial and deleterious. Under normal physiological conditions, ROS production is tightly regulated, and ROS participate in both pathogen defense and cellular signaling. However, insufficient ROS detoxification or ROS overproduction generates oxidative stress, resulting in cellular damage. Oxidative stress has been linked to various inflammatory diseases. Inflammation is an essential response in the protection against injurious insults and thus important at the onset of wound healing. However, hampered resolution of inflammation can result in a chronic, exaggerated response with additional tissue damage. In the pathogenesis of several inflammatory skin conditions, e.g., sunburn and psoriasis, inflammatory-mediated tissue damage is central. The prolonged release of excess ROS in the skin can aggravate inflammatory injury and promote chronic inflammation. The cellular redox balance is therefore tightly regulated by several (enzymatic antioxidants and pro-oxidants; however, in case of chronic inflammation, the antioxidant system may be depleted, and prolonged oxidative stress occurs. Due to the central role of ROS in inflammatory pathologies, restoring the redox balance forms an innovative therapeutic target in the development of new strategies for treating inflammatory skin conditions. Nevertheless, the clinical use of antioxidant-related therapies is still in its infancy.

  16. Redox conditions and protein oxidation in plant mitochondria

    DEFF Research Database (Denmark)

    Møller, Ian Max; Kasimova, Marina R.; Krab, Klaas

    2005-01-01

    Redox conditions and protein oxidation in plant mitochondria NAD(P)H has a central position in respiratory metabolism. It is produced by a large number of enzymes, e.g. the Krebs cycle dehydrogenases, in the mitochondrial matrix and is oxidised by, amongst others, the respiratory chain. Most...... of this NAD(P)H appears to be bound to proteins, in fact free NAD(P)H – an important parameter in metabolic regulation - has never been observed in mitochondria. We have estimated free and bound NAD(P)H in isolated plant mitochondria under different metabolic conditions. The fluorescence spectra of free...... and bound NADH was determined and used to deconvolute fluorescence spectra of actively respiring mitochondria. Most of the mitochondrial NADH is bound in states 2 and 4. The amount of free NADH is lower but relatively constant even increasing a little in state 3 where it is about equal to bound NADH...

  17. Field scale heterogeneity of redox conditions in till-upscaling to a catchment nitrate model

    DEFF Research Database (Denmark)

    Hansen, J.R.; Erntsen, V.; Refsgaard, J.C.

    2008-01-01

    Point scale studies in different settings of glacial geology show a large local variation of redox conditions. There is a need to develop an upscaling methodology for catchment scale models. This paper describes a study of field-scale heterogeneity of redox-interfaces in a till aquitard within an...

  18. Redox Fluctuation Influences Viral Abundance in the Reduced Zone of a Shallow Alluvial Aquifer in Rifle, CO

    Science.gov (United States)

    Pan, D.; Williams, K. H.; Robbins, M.; Weber, K. A.

    2014-12-01

    Naturally reduced zones (NRZs) within alluvial aquifers contain naturally elevated concentrations of organic carbon (OC) and can play a role in controlling the fate of redox-active contaminants. OC in NRZs stimulates microbial activity through coupling of OC oxidation to reduction of subsurface electron acceptors. Stimulation of the indigenous microbial community also results in the stimulation of the viral community. Viruses are the most abundant biological entity on Earth and can indirectly influence carbon cycling by infecting and lysing host cells, resulting in release of OC bound in biomass. In the alluvial aquifer near Rifle, CO, prior acetate injection resulted in reductive immobilization of U and a reduced zone simulating a NRZ, with elevated ferrous iron and sulfide (53.2-62.5 µM and 0.2-3.1 µM, respectively). To study the effects of redox fluctuations in a RZ, oxygenated groundwater was injected. Prior to injection, groundwater was suboxic (0.05-0.11 mg/L). Amended O2 was immediately consumed in the RZ. While cell numbers didn't significantly increase, viruses increased from 1.1x106-2.1x106 mL-1 to 2.3x106-4.6x106 mL-1. VCR increased 1.8-3.4 fold from 3.9-10.1 to 11.0-17.9, demonstrating microbial activity. These changes were associated with large fluctuations of groundwater dissolved OC, suggesting viral release of OC from cellular biomass. Groundwater ORP decreased from an initial -146 mV - -132 mV to -317 mV - -304 mV, indicating an increase in the supply of available electron donors. Thus, rather than expected oxidative solubilization of U following amendment of O2, soluble U decreased, suggesting likely U reduction in the RZ. Fe and S fluctuated, but changes were not associated with aqueous U. Across the whole floodplain, viral abundance is correlated to groundwater dissolved OC, suggesting that viruses may be contributing to the liberalization of dissolved OC from biomass in NRZs, allowing turnover of carbon and reduction of contaminants of

  19. Effect of redox conditions on pharmaceutical loss during biological wastewater treatment using sequencing batch reactors

    Energy Technology Data Exchange (ETDEWEB)

    Stadler, Lauren B., E-mail: lstadler@umich.edu [Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, EWRE, Ann Arbor, MI 48109 (United States); Su, Lijuan, E-mail: lijuansu@buffalo.edu [Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260 (United States); Moline, Christopher J., E-mail: christopher.moline@hdrinc.com [Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, EWRE, Ann Arbor, MI 48109 (United States); Ernstoff, Alexi S., E-mail: alexer@dtu.dk [Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, EWRE, Ann Arbor, MI 48109 (United States); Aga, Diana S., E-mail: dianaaga@buffalo.edu [Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260 (United States); Love, Nancy G., E-mail: nglove@umich.edu [Department of Civil and Environmental Engineering, University of Michigan, 1351 Beal Avenue, EWRE, Ann Arbor, MI 48109 (United States)

    2015-01-23

    Highlights: • Pharmaceutical fate was studied in SBRs operated at different redox conditions. • Stable carbon oxidation and nitrification occurred under microaerobic conditions. • Losses of atenolol and trimethoprim were highest under fully aerobic conditions. • Loss of sulfamethoxazole was highest under microaerobic conditions. • Deconjugation occurred during treatment to form sulfamethoxazole and desvenlafaxine. - Abstract: We lack a clear understanding of how wastewater treatment plant (WWTP) process parameters, such as redox environment, impact pharmaceutical fate. WWTPs increasingly install more advanced aeration control systems to save energy and achieve better nutrient removal performance. The impact of redox condition, and specifically the use of microaerobic (low dissolved oxygen) treatment, is poorly understood. In this study, the fate of a mixture of pharmaceuticals and several of their transformation products present in the primary effluent of a local WWTP was assessed in sequencing batch reactors operated under different redox conditions: fully aerobic, anoxic/aerobic, and microaerobic (DO concentration ≈0.3 mg/L). Among the pharmaceuticals that were tracked during this study (atenolol, trimethoprim, sulfamethoxazole, desvenlafaxine, venlafaxine, and phenytoin), overall loss varied between them and between redox environments. Losses of atenolol and trimethoprim were highest in the aerobic reactor; sulfamethoxazole loss was highest in the microaerobic reactors; and phenytoin was recalcitrant in all reactors. Transformation products of sulfamethoxazole and desvenlafaxine resulted in the reformation of their parent compounds during treatment. The results suggest that transformation products must be accounted for when assessing removal efficiencies and that redox environment influences the degree of pharmaceutical loss.

  20. TIC/TOC and Redox Sensitive Trace Element (RSTEs) Signals Indicating Redox Conditions of the Lower Part of the Cabo Formation Near Organya (Organya Basin), Catalunya, Spain

    Science.gov (United States)

    Herdocia, C.; Maurrasse, F. J.

    2017-12-01

    The thick (> 4.5 km) sedimentary succession of the Organya Basin includes the Cabo Formation [1] which is well exposed in the Cabo valley area and is characteristically composed of black to dark gray marlstones and limestones that accumulated during the greenhouse climate and contain variable amount of organic matter [2-4]. Here we present geochemical results to assess redox conditions of 35.6 m of the Cabo Formation near the Barremian / Aptian boundary, along Catalunya Route C-14, immediately north of the town of Organya. TOC values range between 1 wt% and 5.8 wt%, and peak in all black limestones (0.43 m, 4.38 m, 14.85 m, 29.95 m, and 35.6 m). These TOC values average about 2.0 wt %, except at a height of 0.43 m, where the TOC has a strong peak (5.78 wt%). TIC values oscillated between 86.7 wt% and 96.8 wt%, and averaged at 92.7 wt% and show a strong negative correlation with TOC (r = -0.78). Measured carbon isotope on the organic carbon fraction (δ13Corg) showed fluctuations that ranged from -24.41‰ to -22.15‰. The TOC and δ13Corg curves show a positive correlation (r = 0.58), suggesting that carbon sequestration in the basin followed the overall global signature. Redox sensitive trace elements (V, Ni, Cu, and Mo) correlate with TOC values (r > 0.6), suggesting that dysoxic conditions were responsible for the preservation of organic matter. Biolimiting trace elements (Fe, P) also correlate positively with redox trace elements, and both have highest concentrations at 14.85 m, in concurrence with a high TOC value (2.93 wt%) indicating high primary productivity at that level. Major elements (Al, Si, and Ti) also correlates slightly with TOC (Al: r = 0.39; Si: r = 0.36; Ti: r = 0.43). References: [1] García-Senz, J., 2002, PhD Thesis, University of Barcelona, 310 pp. [2] Bernaus, J.M., et al., 2003. Sedimentary Geology 159 (3-4), 177-201. [3] Caus, E., et al., 1990. Cret. Research 11, 313-320. [4] Sanchez-Hernandez, Y., Maurrasse, F.J-M.R. 2014. Chem

  1. Very high gravity ethanol fermentation by flocculating yeast under redox potential-controlled conditions

    Directory of Open Access Journals (Sweden)

    Liu Chen-Guang

    2012-08-01

    Full Text Available Abstract Background Very high gravity (VHG fermentation using medium in excess of 250 g/L sugars for more than 15% (v ethanol can save energy consumption, not only for ethanol distillation, but also for distillage treatment; however, stuck fermentation with prolonged fermentation time and more sugars unfermented is the biggest challenge. Controlling redox potential (ORP during VHG fermentation benefits biomass accumulation and improvement of yeast cell viability that is affected by osmotic pressure and ethanol inhibition, enhancing ethanol productivity and yield, the most important techno-economic aspect of fuel ethanol production. Results Batch fermentation was performed under different ORP conditions using the flocculating yeast and media containing glucose of 201 ± 3.1, 252 ± 2.9 and 298 ± 3.8 g/L. Compared with ethanol fermentation by non-flocculating yeast, different ORP profiles were observed with the flocculating yeast due to the morphological change associated with the flocculation of yeast cells. When ORP was controlled at −100 mV, ethanol fermentation with the high gravity (HG media containing glucose of 201 ± 3.1 and 252 ± 2.9 g/L was completed at 32 and 56 h, respectively, producing 93.0 ± 1.3 and 120.0 ± 1.8 g/L ethanol, correspondingly. In contrast, there were 24.0 ± 0.4 and 17.0 ± 0.3 g/L glucose remained unfermented without ORP control. As high as 131.0 ± 1.8 g/L ethanol was produced at 72 h when ORP was controlled at −150 mV for the VHG fermentation with medium containing 298 ± 3.8 g/L glucose, since yeast cell viability was improved more significantly. Conclusions No lag phase was observed during ethanol fermentation with the flocculating yeast, and the implementation of ORP control improved ethanol productivity and yield. When ORP was controlled at −150 mV, more reducing power was available for yeast cells to survive, which in turn improved their viability and VHG

  2. The effect of different chemical treatments, pyrolysis conditions and feedstocks on the redox properties of biochar.

    Science.gov (United States)

    Chacón, Francisco Javier; Cayuela, María Luz; Roig, Asunción; Ángel Sánchez-Monedero, Miguel

    2017-04-01

    Pyrogenic carbonaceous materials can have a role in several biogeochemical redox reactions as electron transfer catalysts. Low N2O emissions in biochar amended soils can be related to its ability to act as an "electron shuttle", facilitating the transport of electrons to soil denitrifying microorganisms. Modifying biochar redox properties could be an interesting approach to regulate this effect. In this work we propose several methods for the development of biochars from slow pyrolysis with altered electrochemical properties. To improve its electron exchange capacity we aimed to: 1) Increase the number of redox active functional groups in biochar. Several pyrolysis conditions and chemical treatments (KOH, H3PO4 and H2O2) were tested. 2) Raise the fraction of redox active mineral in biochar. The presence of Fe and Mn-based minerals in biochar could also catalyze redox reactions in soil associated with the nitrogen cycle. Different additives (FeCl3, KMnO4 and clay) were combined with the feedstock before the pyrolysis process. Results of their ability to modify biochar redox properties, measured by mediated electrochemical analysis, are presented. Additionally, we characterized biochars produced from different feedstocks to assess how their lignin, holocellulose and ash composition affects these properties. Analytical issues arising from the difficulty of measuring the electron exchange capacity of biochar will also be discussed.

  3. Groundwater redox conditions and conductivity in a contaminant plume from geoelectrical investigations

    Directory of Open Access Journals (Sweden)

    V. Naudet

    2004-01-01

    Full Text Available Accurate mapping of the electrical conductivity and of the redox potential of the groundwater is important in delineating the shape of a contaminant plume. A map of redox potential in an aquifer is indicative of biodegradation of organic matter and of concentrations of redox-active components; a map of electrical conductivity provides information on the mineralisation of the groundwater. Both maps can be used to optimise the position of pumping wells for remediation. The self-potential method (SP and electrical resistivity tomography (ERT have been applied to the contaminant plume associated with the Entressen landfill in south-east France. The self-potential depends on groundwater flow (electrokinetic contribution and redox conditions ('electro-redox' contribution. Using the variation of the piezometric head in the aquifer, the electrokinetic contribution is removed from the SP signals. A good linear correlation (R2=0.85 is obtained between the residual SP data and the redox potential values measured in monitoring wells. This relationship is used to draw a redox potential map of the overall contaminated site. The electrical conductivity of the subsoil is obtained from 3D-ERT analysis. A good linear correlation (R2=0.91 is observed between the electrical conductivity of the aquifer determined from the 3D-ERT image and the conductivity of the groundwater measured in boreholes. This indicates that the formation factor is nearly homogeneous in the shallow aquifer at the scale of the ERT. From this correlation, a map of the pore water conductivity of the aquifer is obtained. Keywords: self-potential, redox potential, electrical resistivity tomography, fluid conductivity, contaminant plume

  4. Copper dynamics under alternating redox conditions is influenced by soil properties and contamination source.

    Science.gov (United States)

    Balint, Ramona; Said-Pullicino, Daniel; Ajmone-Marsan, Franco

    2015-02-01

    Understanding the effect of soil redox conditions on contaminant dynamics is of significant importance for evaluating their lability, mobility and potential transfer to other environmental compartments. Under changing redox conditions, soil properties and constituents such as Fe and Mn (hydr)oxides and organic matter (OM) may influence the behavior of associated metallic elements (MEs). In this work, the redox-driven release and redistribution of Cu between different soil pools was studied in three soils having different contamination sources. This was achieved by subjecting soil columns to a series of alternating reducing and oxidizing cycles under non-limiting C conditions, and assessing their influence on soil pore water, leachate and solid phase composition. Results showed that, in all soils, alternating redox conditions led to an increase in the distribution of Cu in the more labile fractions, consequently enhancing its susceptibility to loss. This was generally linked to the redox-driven cycling of Fe, Mn and dissolved organic matter (DOM). In fact, results suggested that the reductive dissolution of Fe and Mn (hydr)oxides and subsequent reprecipitation as poorly-ordered phases under oxic conditions contributed to the release and mobilization of Cu and/or Cu-containing organometallic complexes. However, the behavior of Cu, as well as the mechanisms controlling Cu release and loss with redox cycling, was influenced by both soil properties (e.g. pH, contents of easily reducible Fe and Mn (hydr)oxides) and source of Cu contamination. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. Targeting the redox balance in inflammatory skin conditions

    NARCIS (Netherlands)

    Wagener, F.A.D.T.G.; Carels, C.E.L.; Lundvig, D.M.S.

    2013-01-01

    Reactive oxygen species (ROS) can be both beneficial and deleterious. Under normal physiological conditions, ROS production is tightly regulated, and ROS participate in both pathogen defense and cellular signaling. However, insufficient ROS detoxification or ROS overproduction generates oxidative

  6. Redox potential dynamics in a horizontal subsurface flow constructed wetland for wastewater treatment: Diel, seasonal ans spatial fluctuations

    Czech Academy of Sciences Publication Activity Database

    Dušek, Jiří; Picek, T.; Čížková, Hana

    2008-01-01

    Roč. 34, - (2008), s. 223-232 ISSN 0925-8574 Institutional research plan: CEZ:AV0Z60870520 Keywords : Redox potential * Redox prosesses * Phragmites australis * Wastewater treatment s * Constructed wetlands * Contunuous measurement Subject RIV: DJ - Water Pollution ; Quality Impact factor: 1.836, year: 2008

  7. Cu/ZnO nanocatalysts in response to environmental conditions: surface morphology, electronic structure, redox state and CO2 activation.

    Science.gov (United States)

    Martínez-Suárez, Luis; Frenzel, Johannes; Marx, Dominik

    2014-12-21

    Methanol synthesis is one of the landmarks of heterogeneous catalysis due to the great industrial significance of methanol as a clean liquid fuel and as a raw material for industry. Understanding in atomistic detail the properties of the underlying metal/oxide catalyst materials as a function of temperature and composition of the reactive gas phase is of utmost importance in order to eventually improve the production process. By performing extensive density functional theory based slab calculations in combination with a thermodynamic formalism we establish an atomistic understanding of gas phase-induced changes of surface morphology, redox properties and reactivity of ZnO supported Cu nanocatalysts. Extending our recent insights [Phys. Rev. Lett., 2013, 110, 086108], we explore surface stabilization mechanisms and site-dependent redox states of both catalyst components as well as the pronounced electronic charge transfer processes across the metal-support interface. Moreover, ab initio molecular dynamics simulations unveil the vital role played by dynamical shape fluctuations of the deposited Cu8 cluster. The pronounced structural flexibility of the metal nanoparticle is found to enhance CO2 activation over Cu8 at the elevated temperature conditions of the industrial methanol synthesis process, in addition to activation of CO2via electronic charge transfer from the ZnO support.

  8. Model development to evaluate evolution of redox conditions in the near field

    International Nuclear Information System (INIS)

    Chiba, Tamotsu; Miki, Takahito; Inagaki, Manabu; Sasamoto, Hiroshi; Yui, Mikazu

    1999-02-01

    Deep underground is thought to be a potential place for high level radioactive waste repository. It is believed that the chemical condition of deep groundwater is generally anoxic and reducing. However, during construction and operation phase of repository, oxygen will diffuse some distance into the surrounding rock mass, and diffused oxygen may remain in the surrounding rock mass even after repository closure. In such a case, the transitional redox condition around the drift is not preferable in view point of safety assessment for HLW disposal. Hence, it is very important to evaluate evolution of redox conditions in the near field. This report describes the status of model development to evaluate evolution of redox conditions in the near field. We use the commercial solver to equate the mathematical equations which mean evolution of redox condition in the near field. The target area modeled in this report are near field rock mass and engineered barrier (buffer). In case of near field rock mass, we consider the following two geological media: (1) porous media for sedimentary rock, (2) fractured media for crystalline rock. In case of the engineered barrier, we regard the buffer as porous media. We simulate the behavior of dissolved oxygen and Fe 2+ in groundwater during evolution of redox condition in the near field rock mass and the buffer. In case of the porous media, we consider diffusion of chemical species as dominant transport mechanism. On the other hand, in case of the fractured media, we consider diffusion of chemical species in rock matrix and advection of that (only dissolved oxygen considered in this model) in fracture as transport mechanism. We also use the rate law of iron oxidation reaction and dissolution of Fe-bearing minerals in this model besides. (author)

  9. Effect of redox conditions on pharmaceutical loss during biological wastewater treatment using sequencing batch reactors

    DEFF Research Database (Denmark)

    Stadler, Lauren B.; Su, Lijuan; Moline, Christopher J.

    2015-01-01

    condition, and specifically the use of microaerobic (low dissolved oxygen) treatment, is poorly understood. In this study, the fate of a mixture of pharmaceuticals and several of their transformation products present in the primary effluent of a local WWTP was assessed in sequencing batch reactors operated......We lack a clear understanding of how wastewater treatment plant (WWTP) process parameters, such as redox environment, impact pharmaceutical fate. WWTPs increasingly install more advanced aeration control systems to save energy and achieve better nutrient removal performance. The impact of redox...... of their parent compounds during treatment. The results suggest that transformation products must be accounted for when assessing removal efficiencies and that redox environment influences the degree of pharmaceutical loss....

  10. Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Anita Ayer

    Full Text Available Responses to many growth and stress conditions are assumed to act via changes to the cellular redox status. However, direct measurement of pH-adjusted redox state during growth and stress has never been carried out. Organellar redox state (E GSH was measured using the fluorescent probes roGFP2 and pHluorin in Saccharomyces cerevisiae. In particular, we investigated changes in organellar redox state in response to various growth and stress conditions to better understand the relationship between redox-, oxidative- and environmental stress response systems. E GSH values of the cytosol, mitochondrial matrix and peroxisome were determined in exponential and stationary phase in various media. These values (-340 to -350 mV were more reducing than previously reported. Interestingly, sub-cellular redox state remained unchanged when cells were challenged with stresses previously reported to affect redox homeostasis. Only hydrogen peroxide and heat stress significantly altered organellar redox state. Hydrogen peroxide stress altered the redox state of the glutathione disulfide/glutathione couple (GSSG, 2H(+/2GSH and pH. Recovery from moderate hydrogen peroxide stress was most rapid in the cytosol, followed by the mitochondrial matrix, with the peroxisome the least able to recover. Conversely, the bulk of the redox shift observed during heat stress resulted from alterations in pH and not the GSSG, 2H(+/2GSH couple. This study presents the first direct measurement of pH-adjusted redox state in sub-cellular compartments during growth and stress conditions. Redox state is distinctly regulated in organelles and data presented challenge the notion that perturbation of redox state is central in the response to many stress conditions.

  11. Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.

    Science.gov (United States)

    Ayer, Anita; Sanwald, Julia; Pillay, Bethany A; Meyer, Andreas J; Perrone, Gabriel G; Dawes, Ian W

    2013-01-01

    Responses to many growth and stress conditions are assumed to act via changes to the cellular redox status. However, direct measurement of pH-adjusted redox state during growth and stress has never been carried out. Organellar redox state (E GSH) was measured using the fluorescent probes roGFP2 and pHluorin in Saccharomyces cerevisiae. In particular, we investigated changes in organellar redox state in response to various growth and stress conditions to better understand the relationship between redox-, oxidative- and environmental stress response systems. E GSH values of the cytosol, mitochondrial matrix and peroxisome were determined in exponential and stationary phase in various media. These values (-340 to -350 mV) were more reducing than previously reported. Interestingly, sub-cellular redox state remained unchanged when cells were challenged with stresses previously reported to affect redox homeostasis. Only hydrogen peroxide and heat stress significantly altered organellar redox state. Hydrogen peroxide stress altered the redox state of the glutathione disulfide/glutathione couple (GSSG, 2H(+)/2GSH) and pH. Recovery from moderate hydrogen peroxide stress was most rapid in the cytosol, followed by the mitochondrial matrix, with the peroxisome the least able to recover. Conversely, the bulk of the redox shift observed during heat stress resulted from alterations in pH and not the GSSG, 2H(+)/2GSH couple. This study presents the first direct measurement of pH-adjusted redox state in sub-cellular compartments during growth and stress conditions. Redox state is distinctly regulated in organelles and data presented challenge the notion that perturbation of redox state is central in the response to many stress conditions.

  12. Redox conditions and protein oxidation in plant mitochondria

    DEFF Research Database (Denmark)

    Møller, Ian Max; Kasimova, Marina R.; Krab, Klaas

    2005-01-01

    . The consequences for the regulation of respiratory metabolism will be considered. When the conditions in the mitochondria are particularly reducing such as in state 4 or in the presence of inhibitors of the respiratory chain the production of Reactive Oxygen Species is increased (Ref. 1). This can lead to damage...... to proteins, lipids and DNA. We are studying the oxidation of proteins. The formation of N-formylkynurenin by dioxygenation of tryptophan was detected in peptides from rice leaf and potato tuber mitochondria by mass spectrometry. Oxidized tryptophan was detected in 27 peptides representing 17 different....... The same site was modified in (i) several adjacent spots containing the P-protein of the glycine decarboxylase complex, (ii) two different isoforms of the mitochondrial processing protease in complex III and (iii) superoxide dismutase in both rice and potato mitochondria. This indicates that tryptophan...

  13. Preliminary analysis for evolution of redox conditions in the near field

    International Nuclear Information System (INIS)

    Chiba, Tamotsu; Miki, Takahito; Inagaki, Manabu; Sasamoto, Hiroshi; Yui, Mikazu

    1999-06-01

    It is planned that high level radioactive waste is going to be disposed under deep geological environment. It is believed that the chemical condition of deep groundwater is generally anoxic and reducing. However, during construction and operation phase of repository, oxygen will diffuse some distance into the surrounding rock mass, and diffused oxygen may remain in the surrounding rock mass even after repository closure. In such a case, the transitional redox condition around the drift is not preferable in view point of safety assessment for HLW disposal. Hence, it is very important to evaluate evolution of redox conditions in the near field. This report describes results of preliminary analysis for evolution of redox conditions in the near field rock mass and buffer after repository closure based on the model developed by Chiba et al. (1999). The results of preliminary analysis are summarized as follows: The decrease of oxygen in the near field rock mass and buffer are affected by pH of groundwater and surface area of iron-bearing minerals. The decrease of oxygen in the near field rock mass takes place at time scales lower than 500 years in considering the hypothetical reference groundwater pH range for H12 report. It is implicated that the redox conditions in the near field rock mass will recover to reducing conditions. The decrease of oxygen in the buffer takes place at time scales lower several tens years under neutral to weakly alkaline pH values of porewater in the buffer, even if it is assumed that residual oxygen in the near field rock mass after repository closure will diffuse into the buffer. On the other hand, under weakly acid pH values of porewater in the buffer, it may be presumed that oxygen remain in the buffer at time scale more than 500 years. (author)

  14. Generating wind fluctuations for Large Eddy Simulation inflow boundary condition

    International Nuclear Information System (INIS)

    Bekele, S.A.; Hangan, H.

    2004-01-01

    Large Eddy Simulation (LES) studies of flows over bluff bodies immersed in a boundary layer wind environment require instantaneous wind characteristics. The influences of the wind environment on the building pressure distribution are a well-established fact in the experimental study of wind engineering. Measured wind data of full or model scale are available only at a limited number of points. A method of obtaining instantaneous wind data at all mesh points of the inlet boundary for LES computation is necessary. Herein previous and new wind inflow generation techniques are presented. The generated wind data is then applied to a LES computation of a channel flow. The characteristics of the generated wind fluctuations in comparison to the measured data and the properties of the flow field computed from these two wind data are discussed. (author)

  15. 3D IMAGING OF THE MITOCHONDRIAL REDOX STATE OF RAT HEARTS UNDER NORMAL AND FASTING CONDITIONS.

    Science.gov (United States)

    Xu, He N; Zhou, Rong; Moon, Lily; Feng, Min; Li, Lin Z

    2014-03-01

    The heart requires continuous ATP availability that is generated in the mitochondria. Although studies using the cell culture and perfused organ models have been carried out to investigate the biochemistry in the mitochondria in response to a change in substrate supply, mitochondrial bioenergetics of heart under normal feed or fasting conditions has not been studied at the tissue level with a sub-millimeter spatial resolution either in vivo or ex vivo . Oxidation of many food-derived metabolites to generate ATP in the mitochondria is realized through the NADH/NAD + couple acting as a central electron carrier. We employed the Chance redox scanner - the low-temperature fluorescence scanner to image the three-dimensional (3D) spatial distribution of the mitochondrial redox states in heart tissues of rats under normal feeding or an overnight starvation for 14.5 h. Multiple consecutive sections of each heart were imaged to map three redox indices, i.e., NADH, oxidized flavoproteins (Fp, including flavin adenine dinucleotide (FAD)) and the redox ratio NADH/Fp. The imaging results revealed the micro-heterogeneity and the spatial distribution of these redox indices. The quantitative analysis showed that in the fasted hearts the standard deviation of both NADH and Fp, i.e., SD_NADH and SD_Fp, significantly decreased with a p value of 0.032 and 0.045, respectively, indicating that the hearts become relatively more homogeneous after fasting. The fasted hearts contained 28.6% less NADH ( p = 0.038). No significant change in Fp was found ( p = 0.4). The NADH/Fp ratio decreased with a marginal p value (0.076). The decreased NADH in the fasted hearts is consistent with the cardiac cells' reliance of fatty acids consumption for energy metabolism when glucose becomes scarce. The experimental observation of NADH decrease induced by dietary restriction in the heart at tissue level has not been reported to our best knowledge. The Chance redox scanner demonstrated the feasibility of 3D

  16. 3D imaging of the mitochondrial redox state of rat hearts under normal and fasting conditions

    Directory of Open Access Journals (Sweden)

    He N. Xu

    2014-03-01

    Full Text Available The heart requires continuous ATP availability that is generated in the mitochondria. Although studies using the cell culture and perfused organ models have been carried out to investigate the biochemistry in the mitochondria in response to a change in substrate supply, mitochondrial bioenergetics of heart under normal feed or fasting conditions has not been studied at the tissue level with a sub-millimeter spatial resolution either in vivo or ex vivo. Oxidation of many food-derived metabolites to generate ATP in the mitochondria is realized through the NADH/NAD+ couple acting as a central electron carrier. We employed the Chance redox scanner — the low-temperature fluorescence scanner to image the three-dimensional (3D spatial distribution of the mitochondrial redox states in heart tissues of rats under normal feeding or an overnight starvation for 14.5 h. Multiple consecutive sections of each heart were imaged to map three redox indices, i.e., NADH, oxidized flavoproteins (Fp, including flavin adenine dinucleotide (FAD and the redox ratio NADH/Fp. The imaging results revealed the micro-heterogeneity and the spatial distribution of these redox indices. The quantitative analysis showed that in the fasted hearts the standard deviation of both NADH and Fp, i.e., SD_NADH and SD_Fp, significantly decreased with a p value of 0.032 and 0.045, respectively, indicating that the hearts become relatively more homogeneous after fasting. The fasted hearts contained 28.6% less NADH (p = 0.038. No significant change in Fp was found (p = 0.4. The NADH/Fp ratio decreased with a marginal p value (0.076. The decreased NADH in the fasted hearts is consistent with the cardiac cells' reliance of fatty acids consumption for energy metabolism when glucose becomes scarce. The experimental observation of NADH decrease induced by dietary restriction in the heart at tissue level has not been reported to our best knowledge. The Chance redox scanner demonstrated the

  17. Subsurface Conditions Controlling Uranium Incorporation in Iron Oxides: A Redox Stable Sink

    Energy Technology Data Exchange (ETDEWEB)

    Fendorf, Scott [Stanford Univ., CA (United States)

    2016-04-05

    Toxic metals and radionuclides throughout the U.S. Department of Energy Complex pose a serious threat to ecosystems and to human health. Of particular concern is the redox-sensitive radionuclide uranium, which is classified as a priority pollutant in soils and groundwaters at most DOE sites owing to its large inventory, its health risks, and its mobility with respect to primary waste sources. The goal of this research was to contribute to the long-term mission of the Subsurface Biogeochemistry Program by determining reactions of uranium with iron (hydr)oxides that lead to long-term stabilization of this pervasive contaminant. The research objectives of this project were thus to (1) identify the (bio)geochemical conditions, including those of the solid-phase, promoting uranium incorporation in Fe (hydr)oxides, (2) determine the magnitude of uranium incorporation under a variety of relevant subsurface conditions in order to quantify the importance of this pathway when in competition with reduction or adsorption; (3) identify the mechanism(s) of U(VI/V) incorporation in Fe (hydr)oxides; and (4) determine the stability of these phases under different biogeochemical (inclusive of redox) conditions. Our research demonstrates that redox transformations are capable of achieving U incorporation into goethite at ambient temperatures, and that this transformation occurs within days at U and Fe(II) concentrations that are common in subsurface geochemical environments with natural ferrihydrites—inclusive of those with natural impurities. Increasing Fe(II) or U concentration, or initial pH, made U(VI) reduction to U(IV) a more competitive sequestration pathway in this system, presumably by increasing the relative rate of U reduction. Uranium concentrations commonly found in contaminated subsurface environments are often on the order of 1-10 μM, and groundwater Fe(II) concentrations can reach exceed 1 mM in reduced zones of the subsurface. The redox-driven U(V) incorporation

  18. Non-equilibrium concentration fluctuations in binary liquids with realistic boundary conditions.

    Science.gov (United States)

    Ortiz de Zárate, J M; Kirkpatrick, T R; Sengers, J V

    2015-09-01

    Because of the spatially long-ranged nature of spontaneous fluctuations in thermal non-equilibrium systems, they are affected by boundary conditions for the fluctuating hydrodynamic variables. In this paper we consider a liquid mixture between two rigid and impervious plates with a stationary concentration gradient resulting from a temperature gradient through the Soret effect. For liquid mixtures with large Lewis and Schmidt numbers, we are able to obtain explicit analytical expressions for the intensity of the non-equilibrium concentration fluctuations as a function of the frequency ω and the wave number q of the fluctuations. In addition we elucidate the spatial dependence of the intensity of the non-equilibrium fluctuations responsible for a non-equilibrium Casimir effect.

  19. Effect of the soil's redox conditions on the mobility of Se

    International Nuclear Information System (INIS)

    Darcheville, O.; Fevrier, L.; Martin-Garin, A.

    2005-01-01

    Full text of publication follows: 79 Se is a long-life fission product found in the nuclear wastes. Understanding its behaviour in soils is of major concern because of its bioavailability with almost all living organisms (plants and animals). This study is part of a wider multi-disciplinary program that aims at studying 'the effect of the geochemical transformations and the microbial activities on the fate of Se in soils' [1]. The first part of this program is focused on the interactions between Se and the organic compartment of soils, including both biotic (microorganisms, plants,...) and abiotic (humic, fulvic substances,...) components. Special attention is paid to their consequence on the mobility of Se. The second part of the study, presented here, aims specifically at understanding the effect of the soil's redox status on the mobility of Se and at identifying the nature of the processes involved (microbiological vs chemical). Different types of experiments are performed under laboratory controlled conditions. They consist in mixing a 2 mm-sieved sandy soil from the Rhone borders (France) with radio-labelled selenite ( 75 SeIV). In batch experiments, the soil is incubated in a closed vessel under various atmospheres (O 2 or N 2 ) and amended with specific nutrients in order to create contrasted redox conditions and to stimulate specific microbial communities. These conditions are also used in column experiments to determine their impact on Se mobility. They are completed by varying the degree of water saturation in the column and the structure of the soil. The percolation front of Se is followed in-situ with a mobile gamma detector without disturbing the system. In both experimental designs, mass recoveries of 75 Se are precisely determined in the soil-solution systems thanks to the quantification of Se concentrations in the aqueous, solid and gaseous phases. In addition, the non reversibly sorbed Se fraction is assessed by performing sequential extractions

  20. Microbial responses to fluctuation of soil aeration status and redox conditions

    Czech Academy of Sciences Publication Activity Database

    Picek, Tomáš; Šimek, Miloslav; Šantrůčková, Hana

    2000-01-01

    Roč. 31, - (2000), s. 315-322 ISSN 0178-2762 R&D Projects: GA ČR GA206/96/0169; GA AV ČR KSK2017602 Institutional research plan: CEZ:AV0Z6066911 Subject RIV: EE - Microbiology, Virology Impact factor: 1.307, year: 2000

  1. Redox conditions effect on flow accelerated corrosion: Influence of hydrazine and oxygen

    International Nuclear Information System (INIS)

    Bouvier, O. de; Bouchacourt, M.; Fruzzetti, K.

    2002-01-01

    Flow accelerated corrosion (FAC) of carbon steels has been studied world-wide for more than twenty years and is now fairly well understood. The influence of several parameters like water chemistry (i.e. pH and oxygen content), temperature, hydrodynamic or mass transfer conditions (i.e. flow velocity, geometry, steam quality..) and steel composition on the corrosion kinetics has been demonstrated both theoretically and experimentally. However, the effect of a reducing environment and variable redox conditions have not yet been fully explored. It's well known that a reducing environment is effective in increasing the resistance of steam generator tubing to intergranular attack / stress corrosion cracking (IGA/SCC) and pitting. In that way, secondary water chemistry specifications have been modified from low hydrazine to high hydrazine chemistry in the steam-water circuit. Nevertheless, increasing hydrazine levels up to 200 μg/kg could have a detrimental effect by potentially enhancing the FAC process. Moreover, in order to have a complete understanding of the possible impact of the water chemistry environment it is also important to consider the impact of redox conditions during shutdowns (cold and/or hot shutdowns) and start up periods when aerated water injections are made to maintain a constant water level in the Steam Generators from the auxiliary feedwater circuit. Therefore, a common EDF and EPRI R and D effort has been recently carried out to study the effects of hydrazine and oxygen on FAC. The results are presented as follows. (authors)

  2. Redox conditions effect on flow accelerated corrosion: Influence of hydrazine and oxygen

    Energy Technology Data Exchange (ETDEWEB)

    Bouvier, O. de [EDF, R and D Div., Moret sur Loing (France); Bouchacourt, M. [EDF, Engineering and Service Div., Villeurbanne (France); Fruzzetti, K. [EPRI, Science and Technology Div., Palo Alto, CA (United States)

    2002-07-01

    Flow accelerated corrosion (FAC) of carbon steels has been studied world-wide for more than twenty years and is now fairly well understood. The influence of several parameters like water chemistry (i.e. pH and oxygen content), temperature, hydrodynamic or mass transfer conditions (i.e. flow velocity, geometry, steam quality..) and steel composition on the corrosion kinetics has been demonstrated both theoretically and experimentally. However, the effect of a reducing environment and variable redox conditions have not yet been fully explored. It's well known that a reducing environment is effective in increasing the resistance of steam generator tubing to intergranular attack / stress corrosion cracking (IGA/SCC) and pitting. In that way, secondary water chemistry specifications have been modified from low hydrazine to high hydrazine chemistry in the steam-water circuit. Nevertheless, increasing hydrazine levels up to 200 {mu}g/kg could have a detrimental effect by potentially enhancing the FAC process. Moreover, in order to have a complete understanding of the possible impact of the water chemistry environment it is also important to consider the impact of redox conditions during shutdowns (cold and/or hot shutdowns) and start up periods when aerated water injections are made to maintain a constant water level in the Steam Generators from the auxiliary feedwater circuit. Therefore, a common EDF and EPRI R and D effort has been recently carried out to study the effects of hydrazine and oxygen on FAC. The results are presented as follows. (authors)

  3. Frequency domain averaging based experimental evaluation of gear fault without tachometer for fluctuating speed conditions

    Science.gov (United States)

    Sharma, Vikas; Parey, Anand

    2017-02-01

    In the purview of fluctuating speeds, gear fault diagnosis is challenging due to dynamic behavior of forces. Various industrial applications employing gearbox which operate under fluctuating speed conditions. For diagnostics of a gearbox, various vibrations based signal processing techniques viz FFT, time synchronous averaging and time-frequency based wavelet transform, etc. are majorly employed. Most of the time, theories about data or computational complexity limits the use of these methods. In order to perform fault diagnosis of a gearbox for fluctuating speeds, frequency domain averaging (FDA) of intrinsic mode functions (IMFs) after their dynamic time warping (DTW) has been done in this paper. This will not only attenuate the effect of fluctuating speeds but will also extract the weak fault feature those masked in vibration signal. Experimentally signals were acquired from Drivetrain Diagnostic Simulator for different gear health conditions i.e., healthy pinion, pinion with tooth crack, chipped tooth and missing tooth and were analyzed for the different fluctuating profiles of speed. Kurtosis was calculated for warped IMFs before DTW and after DTW of the acquired vibration signals. Later on, the application of FDA highlights the fault frequencies present in the FFT of faulty gears. The result suggests that proposed approach is more effective towards the fault diagnosing with fluctuating speed.

  4. Mobilization and Release of colloidal Carbon from a Soil Column Under Redox Oscillation Condition

    Science.gov (United States)

    Afsar, M. Z.; Jin, Y.

    2015-12-01

    Dissolved organic matter (DOM), the most mobile form of carbon (C), strongly influences the cycling, distribution and behavior of C in soil. In wetlands, the reductive dissolution of iron and manganese oxy-hydroxides releases large quantities of DOM into the soil solution. The objective of this study is to quantify the changes in aqueous organic carbon concentration in different sized fractions induced by reduction of iron and increase in pH. Twenty four cm long soil columns were prepared. Columns were run under oxic (as control) and anoxic conditions. Two platinum redox probes were inserted at 10 and 17 cm depths from the soil surface to monitor the redox status of the column. Anoxic and oxic conditions were maintained by flushing with either nitrogen or oxygen gas through the soil. No additional organic sources were added. After 35 days of anoxic environment, column leachate samples were separated by differential centrifugation into five colloidal sized fractions ( 450 nm), 0.59-5.12% (450-220 nm), 0.45-4.91% (220-100 nm), 0.18-2.91% (100-50 nm), 15.48-35.23% (50 nm - 2.3 nm) and 49.15-63.94% (<2.3 nm). The preliminary results confirmed the release of more nanoparticulate (50-2.3 nm) and truly dissolved (<2.3 nm) organic matter from the anoxic soil column. This study will allow us to systematically evaluate the contribution of colloid mobilization on C release and the distribution of different sized fractions of C under redox dynamic environment.

  5. Role of biotransformation, sorption and mineralization of 14C-labelled sulfamethoxazole under different redox conditions

    International Nuclear Information System (INIS)

    Alvarino, T.; Nastold, P.; Suarez, S.; Omil, F.; Corvini, P.F.X.; Bouju, H.

    2016-01-01

    14 C-sulfamethoxazole biotransformation, sorption and mineralization was studied with heterotrophic and autotrophic biomass under aerobic and anoxic conditions, as well as with anaerobic biomass. The 14 C-radiolabelled residues distribution in the solid, liquid and gas phases was closely monitored along a total incubation time of 190 h. Biotransformation was the main removal mechanism, mineralization and sorption remaining below 5% in all the cases, although the presence of a carbon source exerted a positive effect on the mineralization rate by the aerobic heterotrophic bacteria. In fact, an influence of the type of primary substrate and the redox potential was observed in all cases on the biotransformation and mineralization rates, since an enhancement of the removal rate was observed when an external carbon source was used as a primary substrate under aerobic conditions, while a negligible effect was observed under nitrifying conditions. In the liquid phases collected from all assays, up to three additional peaks corresponding to 14 C-radiolabelled residues were detected. The highest concentration was observed under anaerobic conditions, where two radioactive metabolites were detected representing each around 15% of the total applied radioactivity after 180 h incubation. One of the metabolites detected under anoxic and anaerobic conditions, is probably resulting from ring cleavage of the isoxazole ring. - Highlights: • New procedure based on 14 C to determine sulfamethoxazole (SMX) removal • Complete SMX mass balances in solid, liquid and gas phases • Quantification of SMX biotransformation, mineralization and sorption • Influence of the primary metabolism and redox potential on SMX removal • SMX metabolites have been detected and a possible chemical structure was proposed.

  6. Instability of bottom-water redox conditions during accumulation of Quaternary sediment in the Japan Sea

    Science.gov (United States)

    Piper, D.Z.; Isaacs, C.M.

    1996-01-01

    The concentrations of Cd, Cr, Cu, Mo, Ni, Sb, U, V, and Zn were measured in early Quaternary sediment (1.32 to 1.08 Ma) from the Oki Ridge in the Japan Sea. The elements were partitioned between a detrital fraction, composed of terrigenous and volcaniclastic aluminosilicate debris, and a marine fraction, composed of biogenic and hydrogenous debris derived from seawater. The most important factors controlling minor-element accumulation rates in the marine fraction were (1) primary productivity in the photic zone, which largely controlled the flux of particulate organic-matter-bound minor elements settling through the water column and onto the seafloor, and (2) bottom-water redox, which determined the suite of elements that accumulated directly from seawater. This marine fraction of minor elements on Oki Ridge recorded six periods of high minor-element abundance. Assuming a constant bulk sediment accumulation rate, each period lasted roughly 5,000 to 10,000 years with a 41,000-year cycle. Accumulation rates of individual elements such as Cd, Mo, and U suggest sulfate-reducing conditions were established in the bottom water during the 10,000-year periods; accumulation rates of Cr and V during the intervening periods are indicative of less reducing, denitrifying conditions. Interelement ratios, for example, Cu:Mo, V:Cr, and Sb:Mo, further reflect bottom-water instability, such that bottom-water redox actually varied from sulfate reducing to denitrifying during the periods of highest minor-element accumulation rates; it varied from denitrifying to oxidizing during the intervening periods. Sediment lithology supports these interpretations of the minor-element distributions; the sediment is finely laminated for several of the periods represented by Cd, Mo, and U maxima and weakly laminated to bioturbated for the intervening periods. The geochemistry of this sediment demonstrates the unambiguous signal of Mo, principally, but of several other minor elements as well in

  7. Redox Conditions and Related Color Change in Eastern Equatorial Pacific Sediments: IODP Site U1334

    Science.gov (United States)

    Kordesch, W. E.; Gussone, N. C.; Hathorne, E. C.; Kimoto, K.; Delaney, M. L.

    2011-12-01

    This study was prompted by a 65 m thick brown-green color change in deep-sea sediments of IODP Site U1334 (0-38 Ma, 4799 m water depth) that corresponds to its equatorial crossing (caused by the Northward movement of the pacific plate). Green sediment is a visual indicator of reducing conditions in sediment due to enhanced organic matter deposition and burial. Here we use geochemical redox indicators to characterize the effect of equatorial upwelling on bottom water. The modern redox signal is captured in porewater profiles (nitrate, manganese, iron, sulfate) while trace metal Enrichment Factors (EF) in bulk sediment (manganese, uranium, molybdenum, rhenium) normalized to the detrital component (titanium) record redox state at burial. To measure export productivity we also measure biogenic barium. Porewater profiles reveal suboxic diagenesis; profiles follow the expected sequence of nitrate, manganese oxide, and iron oxide reduction with increasing depth. Constant sulfate (~28 μM) implies anoxia has not occurred. Bulk sediment Mn EF are enriched (EF > 1) throughout the record (Mn EF = 15-200) while U and Mo enrichment corresponds to green color and equatorial proximity (U EF = 4-19; Mo EF = 0-7). Constant Mn enrichment implies continuous oxygenation. Uranium and Mo enrichment near the equator represents suboxic conditions also seen in the porewater. Low Re concentrations (below detection) provide additional evidence against anoxia. A comparison of Mn EF from total digestions to samples treated with an additional reductive cleaning step distinguishes between Mn-oxides and Mn-carbonates, indicating oxygenated and reducing conditions respectively. Mn-carbonate occurrence agrees with U and Mo EF; conditions were more reducing near the equator. Bio-Ba shows significant variability over this interval (22-99 mmol g-1). Our geochemical results indicate that bottom waters became suboxic at the equator as a result of equatorial upwelling-influenced increases in organic

  8. Experimental investigation of factors affecting the control of redox conditions within a radwaste repository

    International Nuclear Information System (INIS)

    Guppy, R.M.; Atkinson, A.

    1991-04-01

    The maximum aqueous concentration of multivalent radioelements in a radwaste repository can be estimated from the expected Eh (the oxidising or reducing tendency of the solution) and pH of the aqueous phase in the repository so long as equilibrium between all oxidising and reducing species can be guaranteed. The objective of the work reported here was to ascertain whether any significant departures from redox equilibrium are likely to arise. Technetium (VII) species were exposed under anaerobic conditions to concentrations of ferrous, hydrogen sulphide and thiosulphate species likely to be present in a repository environment to establish which species are capable of reducing aqueous Tc(VII) to a less soluble Tc(IV) solid compound. Potential catalytic solid phases and phases capable of electron exchange were also exposed to Tc(VII) species under anaerobic, aerobic and hydrogen atmospheres. We have not been able to demonstrate conclusively that mutual equilibrium was attained between technetium and iron redox couples, nor that the apparent solubility of technetium was that expected from the resulting Eh and pH of the solution, although some technetium was removed from solution. Hydrogen did not reduce Tc(VII) within the timescale of the experiments and no catalytic effects by haematite or a cementitious backfill grout for reductions involving hydrogen were observable. Magnetite removed some technetium from solution, apparently by surface reaction, under inert (argon) and reducing (hydrogen) atmospheres. Sulphides, and to a lesser extent thiosulphates, will reduce the solubility of technetium to a very low level. (author)

  9. Crude oil degradation by bacterial consortia under four different redox and temperature conditions.

    Science.gov (United States)

    Xiong, Shunzi; Li, Xia; Chen, Jianfa; Zhao, Liping; Zhang, Hui; Zhang, Xiaojun

    2015-02-01

    There is emerging interest in the anaerobic degradation of crude oil. However, there is limited knowledge about the geochemical effects and microbiological activities for it. A mixture of anaerobic sludge and the production water from an oil well was used as an inoculum to construct four consortia, which were incubated under sulfate-reducing or methanogenic conditions at either mesophilic or thermophilic temperatures. Significant degradation of saturated and aromatic hydrocarbons and the changing quantities of some marker compounds, such as pristane, phytane, hopane and norhopane, and their relative quantities, suggested the activity of microorganisms in the consortia. Notably, the redox conditions and temperature strongly affected the diversity and structure of the enriched microbial communities and the oil degradation. Although some specific biomarker showed larger change under methanogenic condition, the degradation efficiencies for total aromatic and saturated hydrocarbon were higher under sulfate-reducing condition. After the 540-day incubation, bacteria of unknown classifications were dominant in the thermophilic methanogenic consortia, whereas Clostridium dominated the mesophilic methanogenic consortia. With the exception of the dominant phylotypes that were shared with the methanogenic consortia, the sulfate-reducing consortia were predominantly composed of Thermotogae, Deltaproteobacteria, Spirochaeta, and Synergistetes phyla. In conclusion, results in this study demonstrated that the different groups of degraders were responsible for degradation in the four constructed crude oil degrading consortia and consequently led to the existence of different amount of marker compounds under these distinct conditions. There might be distinct metabolic mechanism for degrading crude oil under sulfate-reducing and methanogenic conditions.

  10. Solar wind fluctuations at large scale - A comparison between low and high solar activity conditions

    Science.gov (United States)

    Bavassano, B.; Bruno, R.

    1991-02-01

    The influence of the sun's activity cycle on the solar wind fluctuations at time scales from 1 hour to 3 days in the inner heliosphere (0.3 to 1 AU) is investigated. Hourly averages of plasma and magnetic field data by Helios spacecraft are used. Since fluctuations behave quite differently with changing scale, the analysis is performed separately for two different ranges in time scale. Between 1 and 6 hours Alfvenic fluctuations and pressure-balanced structures are extensively observed. At low solar activity and close to 0.3 AU Alfvenic fluctuations are more frequent than pressure-balanced structures. This predominance, however, weakens for rising solar activity and radial distance, to the point that a role-exchange, in terms of occurrence rate, is found at the maximum of the cycle close to 1 AU. On the other hand, in all cases Alfvenic fluctuations have a larger amplitude than pressure-balanced structures. The Alfvenic contribution to the solar wind energy spectrum comes out to be dominant at all solar activity conditions. These findings support the conclusion that the solar cycle evolution of the large-scale velocity pattern is the factor governing the observed variations.

  11. Role of biotransformation, sorption and mineralization of (14)C-labelled sulfamethoxazole under different redox conditions.

    Science.gov (United States)

    Alvarino, T; Nastold, P; Suarez, S; Omil, F; Corvini, P F X; Bouju, H

    2016-01-15

    (14)C-sulfamethoxazole biotransformation, sorption and mineralization was studied with heterotrophic and autotrophic biomass under aerobic and anoxic conditions, as well as with anaerobic biomass. The (14)C-radiolabelled residues distribution in the solid, liquid and gas phases was closely monitored along a total incubation time of 190 h. Biotransformation was the main removal mechanism, mineralization and sorption remaining below 5% in all the cases, although the presence of a carbon source exerted a positive effect on the mineralization rate by the aerobic heterotrophic bacteria. In fact, an influence of the type of primary substrate and the redox potential was observed in all cases on the biotransformation and mineralization rates, since an enhancement of the removal rate was observed when an external carbon source was used as a primary substrate under aerobic conditions, while a negligible effect was observed under nitrifying conditions. In the liquid phases collected from all assays, up to three additional peaks corresponding to (14)C-radiolabelled residues were detected. The highest concentration was observed under anaerobic conditions, where two radioactive metabolites were detected representing each around 15% of the total applied radioactivity after 180 h incubation. One of the metabolites detected under anoxic and anaerobic conditions, is probably resulting from ring cleavage of the isoxazole ring. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Applying linear discriminant analysis to predict groundwater redox conditions conducive to denitrification

    Science.gov (United States)

    Wilson, S. R.; Close, M. E.; Abraham, P.

    2018-01-01

    Diffuse nitrate losses from agricultural land pollute groundwater resources worldwide, but can be attenuated under reducing subsurface conditions. In New Zealand, the ability to predict where groundwater denitrification occurs is important for understanding the linkage between land use and discharges of nitrate-bearing groundwater to streams. This study assesses the application of linear discriminant analysis (LDA) for predicting groundwater redox status for Southland, a major dairy farming region in New Zealand. Data cases were developed by assigning a redox status to samples derived from a regional groundwater quality database. Pre-existing regional-scale geospatial databases were used as training variables for the discriminant functions. The predictive accuracy of the discriminant functions was slightly improved by optimising the thresholds between sample depth classes. The models predict 23% of the region as being reducing at shallow depths (<15 m), and 37% at medium depths (15-75 m). Predictions were made at a sub-regional level to determine whether improvements could be made with discriminant functions trained by local data. The results indicated that any gains in predictive success were offset by loss of confidence in the predictions due to the reduction in the number of samples used. The regional scale model predictions indicate that subsurface reducing conditions predominate at low elevations on the coastal plains where poorly drained soils are widespread. Additional indicators for subsurface denitrification are a high carbon content of the soil, a shallow water table, and low-permeability clastic sediments. The coastal plains are an area of widespread groundwater discharge, and the soil and hydrology characteristics require the land to be artificially drained to render the land suitable for farming. For the improvement of water quality in coastal areas, it is therefore important that land and water management efforts focus on understanding hydrological

  13. Determination of Pressure Fluctuations in Rotor Bundle of Centrifugal Compressor at Critical Conditions of Operation

    Science.gov (United States)

    Levashov, V. A.; Lyubchenko, K. Yu

    2017-08-01

    This article describes the physical processes that occur in the stage flow part of the compressor while it is operating and can create conditions for the occurrence of forced vibrations, which in turn can lead to the destruction of the impellers. Critical conditions of compressor operation are determined. To understand that critical condition of operation is cause of the destruction of the impellers, transient CFD analysis was carried for test stage of compressor. The obtained pressure fluctuation amplitudes allow to evaluate the critical conditions of compressor operation.

  14. Long-term competitive dynamics of two cryptic rotifer species: diapause and fluctuating conditions.

    Directory of Open Access Journals (Sweden)

    Carmen Gabaldón

    Full Text Available Life-history traits may have an important role in promoting species coexistence. However, the complexity of certain life cycles makes it difficult to draw conclusions about the conditions for coexistence or exclusion based on the study of short-term competitive dynamics. Brachionus plicatilis and B. manjavacasare two cryptic rotifer species co-occurring in many lakes on the Iberian Peninsula. They have a complex life cycle in which cyclical parthenogenesis occurs with diapausing stages being the result of sexual reproduction. B. plicatilis and B. manjavacasare identical in morphology and size, their biotic niches are broadly overlapping, and they have similar competitive abilities. However, the species differ in life-history traits involving sexual reproduction and diapause, and respond differently to salinity and temperature. As in the case of certain other species that are extremely similar in morphology, a fluctuating environment are considered to be important for their coexistence. We studied the long-term competitive dynamics of B. plicatilis and B. manjavacas under different salinity regimes (constant and fluctuating. Moreover, we focused on the dynamics of the diapausing egg bank to explore how the outcome of the entire life cycle of these rotifers can work to mediate stable coexistence. We demonstrated that these species do not coexist under constant-salinity environment, as the outcome of competition is affected by the level of salinity-at low salinity, B. plicatilis excluded B. manjavacas, and the opposite outcome occurred at high salinity. Competitive dynamics under fluctuating salinity showed that the dominance of one species over the other also tended to fluctuate. The duration of co-occurrence of these species was favoured by salinity fluctuation and perhaps by the existence of a diapausing egg bank. Stable coexistence was not found in our system, which suggests that other factors or other salinity fluctuation patterns might act as

  15. A novelty detection diagnostic methodology for gearboxes operating under fluctuating operating conditions using probabilistic techniques

    Science.gov (United States)

    Schmidt, S.; Heyns, P. S.; de Villiers, J. P.

    2018-02-01

    In this paper, a fault diagnostic methodology is developed which is able to detect, locate and trend gear faults under fluctuating operating conditions when only vibration data from a single transducer, measured on a healthy gearbox are available. A two-phase feature extraction and modelling process is proposed to infer the operating condition and based on the operating condition, to detect changes in the machine condition. Information from optimised machine and operating condition hidden Markov models are statistically combined to generate a discrepancy signal which is post-processed to infer the condition of the gearbox. The discrepancy signal is processed and combined with statistical methods for automatic fault detection and localisation and to perform fault trending over time. The proposed methodology is validated on experimental data and a tacholess order tracking methodology is used to enhance the cost-effectiveness of the diagnostic methodology.

  16. The evolution of redox conditions and groundwater geochemistry in recharge-discharge environments on the Canadian Shield

    International Nuclear Information System (INIS)

    Gascoyne, M.

    1996-10-01

    Groundwater composition evolves along flow paths from recharge to discharge in response to interactions with bedrock and fracture-filling minerals, and dissolution of soluble (Cl-rich) salts in the rock matrix. The groundwater redox potential changes from oxidizing to reducing conditions due, initially, to rapid consumption of dissolved oxygen by organics in the upper ∼100 m of bedrock and, subsequently, interaction with Fe (II)-containing minerals. Measured Eh values of groundwaters at depth in the granitic Lac du Bonnet batholith indicate that biotite and chlorite control groundwater redox potential. This is supported by other geochemical characteristics such as absence of CH 4 , H 2 S, H 2 , NO 3 , low concentrations of Fe (II), and abundance of SO 4 . Further evidence of evolution of redox conditions is given by variations in U concentration ranging from up to 1000 μg/L in dilute near-surface waters to <1 μg/L in some deep, saline groundwaters. Groundwaters at about 400 m depth in a recharge area on the Lac du Bonnet batholith contain significantly more U than groundwaters further along the flow path or near surface in discharge areas. Uranium concentration is found to be a useful and sensitive indicator of redox conditions. (author)

  17. RANS simulation of cavitation and hull pressure fluctuation for marine propeller operating behind-hull condition

    Directory of Open Access Journals (Sweden)

    Kwang-Jun Paik

    2013-12-01

    Full Text Available Simulations of cavitation flow and hull pressure fluctuation for a marine propeller operating behind a hull using the unsteady Reynolds-Averaged Navier-Stokes equations (RANS are presented. A full hull body submerged under the free surface is modeled in the computational domain to simulate directly the wake field of the ship at the propeller plane. Simulations are performed in design and ballast draught conditions to study the effect of cavitation number. And two propellers with slightly different geometry are simulated to validate the detectability of the numerical simulation. All simulations are performed using a commercial CFD software FLUENT. Cavitation patterns of the simulations show good agreement with the experimental results carried out in Samsung CAvitation Tunnel (SCAT. The simulation results for the hull pressure fluctuation induced by a propeller are also compared with the experimental results showing good agreement in the tendency and amplitude, especially, for the first blade frequency.

  18. Conditional analysis of floating potential fluctuations at the edge of the Texas Experimental Tokamak Upgrade (TEXT-U)

    International Nuclear Information System (INIS)

    Filippas, A.V.; Bengston, R.D.; Li, G.; Meier, M.; Ritz, C.P.; Powers, E.J.

    1995-01-01

    Fluctuations in floating potential in the scrape-off layer and plasma edge were analyzed using a conditional statistical analysis technique. The floating potential fluctuations had a nearly Gaussian probability density function with the largest deviation from a Gaussian at the shear layer. The conditional averaging technique followed the statistical evolution of selected conditions in the floating potential signal. The decay rate of a conditional feature in time or space showed a small systematic variation with the amplitude of condition chosen. Either long-lived coherent structures are not present in statistically significant numbers, or the fluctuations are dominated by a large number of coherent structures with a nearly Gaussian distribution of fluctuation amplitudes, or conditional analysis using the amplitude of the floating potential as a condition is not a sensitive technique for identifying coherent structures

  19. Reconstruction of Redox Conditions and Productivity in Coastal Waters of the Bothnian Sea during the Holocene

    Science.gov (United States)

    Dijkstra, N.; Quintana Krupinski, N. B.; Slomp, C. P.

    2014-12-01

    Hypoxia is a growing problem in coastal waters worldwide, and is a well-known cause of benthic mortality. The semi-enclosed Baltic Sea is currently the world's largest human-induced dead zone. During the early Holocene, it experienced several periods of natural hypoxia following the intrusion of seawater into the previous freshwater lake. Recent studies suggest that at that time, the hypoxia expanded north to include the deep basin of the Bothnian Sea. In this study, we assess whether the coastal zone of the Bothnian Sea was also hypoxic during the early Holocene. We analysed a unique sediment record (0 - 30 mbsf) from the Ångermanälven estuary, which was retrieved during the International Ocean Discovery Programme (IODP) Baltic Sea Paleoenvironment Expedition 347 in 2013. Using geochemical proxies and foraminifera abundances, we reconstruct the changes in redox conditions, salinity and productivity in the estuary. Our preliminary results suggest that bottom waters in this coastal basin became anoxic upon the intrusion of brackish seawater in the early Holocene and that the productivity was elevated. The presence of benthic foraminifera in this estuary during the mid-Holocene suggests more saline conditions in the Bothnian Sea than today. Due to isostatic uplift, the estuary likely gradually became more isolated from the Bothnian Sea, which itself became more isolated from the Baltic Sea. Both factors likely explain the subsequent re-oxygenation of bottom waters and gradual refreshening of the estuary as recorded in the sediments. Interestingly, the upper meters of sediment are enriched in minerals that contain iron, phosphorus and manganese. We postulate that the refreshening of the estuary triggered the formation of these minerals, thereby increasing the phosphorus retention in these sediments and further reducing primary productivity. This enhanced retention linked to refreshening may contribute to the current oligotrophic conditions in the Bothnian Sea.

  20. Bacterial degradation of pyridine, indole, quinoline, and their derivatives under different redox conditions

    Energy Technology Data Exchange (ETDEWEB)

    Fetzner, S. [Oldenburg Univ. (Germany). Fachbereich 7 - Biologie

    1998-08-01

    Bacteria have evolved a diverse potential to transform and even mineralize numerous organic compounds of both natural and xenobiotic origin. This article describes the occurrence of N-heteroaromatic compounds and presents a review of the bacterial degradation of pyridine and its derivatives, indole, insoquinoline, and quinoline and its derivatives. The bacterial metabolism of these compounds under different redox conditions - by aerobic, nitrate-reducing, sulfate-reducing and methanogenic bacteria - is discussed. However, in natural habitats, various environmental factors, such as sorption phenomena, also influence bacterial conversion processes. Thus, both laboratory and field studies are necessary to aid our understanding of biodegradation in natural ecosystems and assist the development of strategies for bioremediation of polluted sites. Occurring predominantly near (former) wood-treatment facilities, creosote is a frequent contaminant of soil, subsoil, groundwater, and aquifer sediments. In situ as well as withdrawal-and-treatment techniques have been designed to remediate such sites, which are polluted with complex mixtures of aromatic and herocyclic compounds. (orig.)

  1. Quantification and characterization of colloids and organic carbon released under oscillating redox conditions

    Science.gov (United States)

    Jin, Yan; Afsar, Mohammad; Yan, Jing

    2017-04-01

    Wetlands account for 8-10% of the world's land surface but their soils contain 20-30% of globe terrestrial carbon. The carbon is intimately mixed with minerals in the soils. Thus, mineral-associated-organic carbon (MOC), which often exists as colloids, can directly affect global carbon cycling at multiple scales. When wetland soils become reduced, large quantities of MOC are released due to dissolution of metal oxides, and mobilized and discharged into adjacent streams during rainfall events. Despite the clear relevance of wetlands to global carbon reservoirs and cycling, MOC, as an important component of wetland carbon pool, is poorly understood. Further, understanding of the key factors controlling the fluxes and compositional characteristics of MOC thus the underlying reaction mechanisms that are responsible for the sequestration and stabilization of OC is also lacking. Here we present results from both field sampling and laboratory experiments on the amount, size distribution, and composition of MOC as influenced by oscillating redox conditions. Using both conventional and advanced analytical techniques, including x-ray photoelectron spectroscopy (XPS) and isotope ratio mass spectroscopy (IRMS), we identify 4 MOC size fractions: 450-1000 nm, 100-450 nm, 2.3-100 nm and < 2.3 nm. Normalized atomic% of different elements obtained from XPS analysis reveal clear variations in mineral and OC compositions in the different size fractions. In particular, the "nano sized" MOC (i.e., 2.3-100 nm fraction) has the highest Mg/Al ratio and OC/mineral ratio, the lowest percentages of Al and Si, is mostly depleted in C-C/C-H functional groups but enriched with C=0 and C-O/C-N groups in contract to other size groups. IRMS analysis shows depletion of the heavier isotope 13C from the 2.3-100 nm fraction indicating the presence of more lignin derivatives in this size fraction. The observed size-dependent heterogeneity on C attachment and release to/from MOC can lead to more

  2. Combining sedimentological, trace metal (Mn, Mo) and molecular evidence for reconstructing past water-column redox conditions: The example of meromictic Lake Cadagno (Swiss Alps)

    DEFF Research Database (Denmark)

    Wirth, S. B.; Gilli, A.; Niemann, H.

    2013-01-01

    processes in a sulfidic environment, possibly depicting analogous conditions in an ancient ocean. Our study underscores the value of combining sedimentological, geochemical, and microbiological approaches to characterize paleo-environmental and -redox conditions in lacustrine and marine settings. (C) 2013...

  3. Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: toward fine-tuning flow in biological microfluidics applications.

    Science.gov (United States)

    Cheah, Lih Tyng; Fritsch, Ingrid; Haswell, Stephen J; Greenman, John

    2012-07-01

    A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs-Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox-magnetohydrodynamics (redox-MHD) conditions. Redox-MHD possesses unique capabilities to control fluid flow using ionic current from oxidation and reduction processes at electrodes in a magnetic field, making it attractive to fine-tune fluid flow around tissues for "tissue-on-a-chip" applications. The manuscript describes a parallel setup to study two tissue samples simultaneously, and 6-min static incubation with Triton X100. Tissue viability was subsequently determined by assaying perfusate for lactate dehydrogenase (LDH) activity, where LDH serves as an injury marker. Incubation with KHGB containing 5 mM hexaammineruthenium(III) (ruhex) redox species with and without a pair of NdFeB magnets (∼ 0.39 T, placed parallel to the chamber) exhibited no additional tissue insult. MHD fluid flow, viewed by tracking microbeads with microscopy, occurred only when the magnet was present and stimulating electrodes were activated. Pulsating MHD flow with a frequency similar to the stimulating waveform was superimposed over thermal convection (from a hotplate) for Triton-KHGB, but fluid speed was up to twice as fast for ruhex-Triton-KHGB. A large transient ionic current, achieved when switching on the stimulating electrodes, generates MHD perturbations visible over varying peristaltic flow. The well-controlled flow methodology of redox-MHD is applicable to any tissue type, being useful in various drug uptake and toxicity studies, and can be combined equally with on- or off-device analysis modalities. Copyright © 2012 Wiley Periodicals, Inc.

  4. Self-organization of critical fluctuations and the 1/f spectra under conditions of critical boiling

    International Nuclear Information System (INIS)

    Skokov, V.N.; Reshetnikov, A.V.; Koverda, V.P.

    2000-01-01

    Paper presents the results of the experimental investigation into evaporation dynamics under change over of water boiling conditions at a platinum wire heater and at the local Joule heating of aqueous electrolyte solutions. Under the film boiling one detected at the vertical heater the generation of thermal fluctuations with spectral characteristics in inverse proportion to frequency (flicker or 1/f-noise). Within the range of stability loss of film boiling the frequency spectra changed in inverse proportion to the frequency square. In the course of the experiments dealing with the Joule heating of the aqueous electrolytes one detected flicker-noise within the range of change-over of vapor generation conditions. The experiment results show the self-implementation of the system critical behaviour under nonequilibrium phase transitions [ru

  5. Synergistic effects of seasonal rainfall, parasites and demography on fluctuations in springbok body condition

    Science.gov (United States)

    Turner, Wendy C.; Versfeld, Wilferd D.; Kilian, J. Werner; Getz, Wayne M.

    2011-01-01

    Summary 1. Seasonality of rainfall can exert a strong influence on animal condition and on host-parasite interactions. The body condition of ruminants fluctuates seasonally in response to changes in energy requirements, foraging patterns and resource availability, and seasonal variation in parasite infections may further alter ruminant body condition. 2. This study disentangles effects of rainfall and gastrointestinal parasite infections on springbok (Antidorcas marsupialis) body condition and determines how these factors vary among demographic groups. 3. Using data from four years and three study areas, we investigated i) the influence of rainfall variation, demographic factors and parasite interactions on parasite prevalence or infection intensity, ii) whether parasitism or rainfall is a more important predictor of springbok body condition and iii) how parasitism and condition vary among study areas along a rainfall gradient. 4. We found that increased parasite intensity is associated with reduced body condition only for adult females. For all other demographic groups, body condition was significantly related to prior rainfall and not to parasitism. Rainfall lagged by two months had a positive effect on body condition. 5. Adult females showed evidence of a “periparturient rise” in parasite intensity, and had higher parasite intensity and lower body condition than adult males after parturition and during early lactation. After juveniles were weaned, adult females had lower parasite intensity than adult males. Sex differences in parasitism and condition may be due to differences between adult females and males in the seasonal timing of reproductive effort and its effects on host immunity, as well as documented sex differences in vulnerability to predation. 6. Our results highlight that parasites and the environment can synergistically affect host populations, but that these interactions might be masked by their interwoven relationships, their differential

  6. Evidence for local and global redox conditions at an Early Ordovician (Tremadocian) mass extinction

    Science.gov (United States)

    Edwards, Cole T.; Fike, David A.; Saltzman, Matthew R.; Lu, Wanyi; Lu, Zunli

    2018-01-01

    Profound changes in environmental conditions, particularly atmospheric oxygen levels, are thought to be important drivers of several major biotic events (e.g. mass extinctions and diversifications). The early Paleozoic represents a key interval in the oxygenation of the ocean-atmosphere system and evolution of the biosphere. Global proxies (e.g. carbon (δ13C) and sulfur (δ34S) isotopes) are used to diagnose potential changes in oxygenation and infer causes of environmental change and biotic turnover. The Cambrian-Ordovician contains several trilobite extinctions (some are apparently local, but others are globally correlative) that are attributed to anoxia based on coeval positive δ13C and δ34S excursions. These extinction and excursion events have yet to be coupled with more recently developed proxies thought to be more reflective of local redox conditions in the water column (e.g. I/Ca) to confirm whether these extinctions were associated with oxygen crises over a regional or global scale. Here we examine an Early Ordovician (Tremadocian Stage) extinction event previously interpreted to reflect a continuation of recurrent early Paleozoic anoxic events that expanded into nearshore environments. δ13C, δ34S, and I/Ca trends were measured from three sections in the Great Basin region to test whether I/Ca trends support the notion that anoxia was locally present in the water column along the Laurentian margin. Evidence for anoxia is based on coincident, but not always synchronous, positive δ13C and δ34S excursions (mainly from carbonate-associated sulfate and less so from pyrite data), a 30% extinction of standing generic diversity, and near-zero I/Ca values. Although evidence for local water column anoxia from the I/Ca proxy broadly agrees with intervals of global anoxia inferred from δ13C and δ34S trends, a more complex picture is evident where spatially and temporally variable local trends are superimposed on time-averaged global trends. Stratigraphic

  7. Modulation of redox homeostasis under suboptimal conditions by Arabidopsis nudix hydrolase 7

    Directory of Open Access Journals (Sweden)

    Jambunathan Niranjani

    2010-08-01

    differences observed in growth phenotypes of the Atnudt7-1 knockout mutants can be due to differences in the nutrient composition of potting mix. Our data suggests AtNUDT7 plays an important role in maintaining redox homeostasis, particularly for maintaining NADH:NAD+ balance for normal growth and development. During stress conditions, rapid induction of AtNUDT7 is important for regulating the activation of stress/defense signaling and cell death pathways.

  8. Effects of biodegradation of mecoprop, dichlorprop and bentazone by changing the redox conditions from anaerobic to aerobic in sandy aquifer

    DEFF Research Database (Denmark)

    Levi, Suzi; Bjerg, Poul Løgstrup; Albrechtsen, Hans-Jørgen

    2011-01-01

    Pesticides and metabolites are detected in an increasing number of aquifers resulting in closure of many drinking water wells. The natural redox conditions of aquifers are considered to be one of the important factors governing the pesticides degradation in groundwater. Water abstraction strategy...... in combination with geological variations may affect microbial degradation processes by mixing in even low oxygen concentrations and create steep gradients and drastic redox zones in aquifers. The aim of the current study was to investigate the stimulation of pesticides biodegradation by adding oxygen...... concentration range 0.0-11mg/L. We have studied effects of oxygen addition to anaerobic sediment on biodegradation of 1 μg/L mecoprop, dichlorprop and bentazone by microcosm experiments in 48 incubations for 130 days. A significant increase of mecoprop degradation was obtained (18-20%) at high oxygen...

  9. Fluctuating and directional asymmetry in young human males: effect of heavy working condition and socioeconomic status.

    Science.gov (United States)

    Ozener, Bariş

    2010-09-01

    Many adverse environmental and genetic factors can affect stability of development during human growth. Although the level of fluctuating asymmetry (FA) may be influenced by environmental and genetic stress encountered during this period, directional asymmetry (DA) is largely attributable to differential mechanical loading during bone growth, for example, handedness. I assessed the effects of heavy working conditions and socioeconomic conditions on asymmetry levels in three groups of young human males: 1) individuals employed in the heavy industry sector (n = 104, mean age = 18.48 +/- 0.61 years), 2) individuals who had the same socioeconomic status as the laborers (n = 102, mean age = 18.39 +/- 0.58 years) but were not laborers, and 3) nonlaborers from the higher socioeconomic levels of society (n = 103, mean age = 18.43 +/- 0.67). For all subjects, hand length, hand width, elbow width, wrist width, knee width, ankle width, foot length, foot width, ear length, and ear width were measured. All measurements of the upper extremities in the labor group appeared to exhibit DA; in the other two groups only hand measurements exhibited DA. According to analysis of FA, subjects living in poor conditions exhibited more FA than their nonlaborer peers living in better conditions. In addition, biomechanical pressures due to heavy working conditions of the labor group appeared to cause increased DA in the upper extremities: DA increased with an increase in the number of years working.

  10. Numerical simulation of pressure fluctuation of a pump-turbine with MGV at no-load condition

    International Nuclear Information System (INIS)

    Liu, J T; Wang, L Q; Liu, S H; Sun, Y K; Wu, Y L

    2012-01-01

    In order to analyse the pressure fluctuation caused by misaligned guide vanes (MGV) during starting period at no-load condition, 3-D (three dimensional), unsteady flows in a pump-turbine were numerically studied. Pressure fluctuations of different points at no-load condition are obtained. Fast Fourier Transform(FFT) was used to analyse the frequency spectrum of pressure fluctuations. The amplitude and dominant frequency of pressure fluctuation at vaneless space between the runner and guide vane, as well as the inlet of draft tube, was investigated. The amplitude of pressure fluctuation of the pump-turbine with MGV device is twice that of synchronous vanes. This might be caused by the non-uniform flow in the pump-turbine due to the pre-opened guide vanes. The pump-turbine with synchronous vanes has a low frequency which is 0.33f n , while the low frequency changes into 0.63f n when the MGV device is used. The vortex rope in the draft tube is large than that of synchronize vanes. Resultsof pressure fluctuations with synchronous vanes agree with each other between computational and testing results. The numerical study of pressure fluctuations with MGV can provide a basic understanding for the improvement of the instability of a pump-turbine.

  11. Prediction and visualization of redox conditions in the groundwater of Central Valley, California

    Science.gov (United States)

    Rosecrans, Celia Z.; Nolan, Bernard T.; Gronberg, JoAnn M.

    2017-03-01

    Regional-scale, three-dimensional continuous probability models, were constructed for aspects of redox conditions in the groundwater system of the Central Valley, California. These models yield grids depicting the probability that groundwater in a particular location will have dissolved oxygen (DO) concentrations less than selected threshold values representing anoxic groundwater conditions, or will have dissolved manganese (Mn) concentrations greater than selected threshold values representing secondary drinking water-quality contaminant levels (SMCL) and health-based screening levels (HBSL). The probability models were constrained by the alluvial boundary of the Central Valley to a depth of approximately 300 m. Probability distribution grids can be extracted from the 3-D models at any desired depth, and are of interest to water-resource managers, water-quality researchers, and groundwater modelers concerned with the occurrence of natural and anthropogenic contaminants related to anoxic conditions. Models were constructed using a Boosted Regression Trees (BRT) machine learning technique that produces many trees as part of an additive model and has the ability to handle many variables, automatically incorporate interactions, and is resistant to collinearity. Machine learning methods for statistical prediction are becoming increasing popular in that they do not require assumptions associated with traditional hypothesis testing. Models were constructed using measured dissolved oxygen and manganese concentrations sampled from 2767 wells within the alluvial boundary of the Central Valley, and over 60 explanatory variables representing regional-scale soil properties, soil chemistry, land use, aquifer textures, and aquifer hydrologic properties. Models were trained on a USGS dataset of 932 wells, and evaluated on an independent hold-out dataset of 1835 wells from the California Division of Drinking Water. We used cross-validation to assess the predictive performance of

  12. Determination of dissolution rates of spent fuel in carbonate solutions under different redox conditions with a flow-through experiment

    International Nuclear Information System (INIS)

    Roellin, S.; Spahiu, K.; Eklund, U.-B.

    2001-01-01

    Dissolution rates of spent UO 2 fuel have been investigated using flow-through experiments under oxidizing, anoxic and reducing conditions. For oxidizing conditions, approximately congruent dissolution rates were obtained in the pH range 3-9.3 for U, Np, Ba, Tc, Cs, Sr and Rb. For these elements, steady-state conditions were obtained in the flow rate range 0.02-0.3 ml min -1 . The dissolution rates were about 3 mg d -1 m -2 for pH>6. For pH 2 (g) saturated solutions dropped by up to four orders of magnitude as compared to oxidizing conditions. Because of the very low concentrations, only U, Pu, Am, Mo, Tc and Cs could be measured. For anoxic conditions, both the redox potential and dissolution rates increased approaching the same values as under oxidizing conditions

  13. Heterogeneous redox conditions, arsenic mobility, and groundwater flow in a fractured-rock aquifer near a waste repository site in New Hampshire, USA

    Science.gov (United States)

    Harte, Philip T.; Ayotte, Joseph D.; Hoffman, Andrew; Revesz, Kinga M.; Belaval, Marcel; Lamb, Steven; Böhlke, J.K.

    2012-01-01

    Anthropogenic sources of carbon from landfill or waste leachate can promote reductive dissolution of in situ arsenic (As) and enhance the mobility of As in groundwater. Groundwater from residential-supply wells in a fractured crystalline-rock aquifer adjacent to a Superfund site in Raymond, New Hampshire, USA, showed evidence of locally enhanced As mobilization in relatively reducing (mixed oxic-anoxic to anoxic) conditions as determined by redox classification and other lines of evidence. Redox classification was determined from geochemical indicators based on threshold concentrations of dissolved oxygen (DO), nitrate (NO3-), iron (Fe2+), manganese (Mn2+), and sulfate (SO42-). Redox conditions were evaluated also based on methane (CH4), excess nitrogen gas (N2) from denitrification, the oxidation state of dissolved As speciation (As(III) and As(V)), and several stable isotope ratios. Samples from the residential-supply wells primarily exhibit mixed redox conditions, as most have long open boreholes (typically 50–100 m) that receive water from multiple discrete fractures with contrasting groundwater chemistry and redox conditions. The methods employed in this study can be used at other sites to gauge redox conditions and the potential for As mobilization in complex fractured crystalline-rock aquifers where multiple lines of evidence are likely needed to understand As occurrence, mobility, and transport.

  14. Effect of redox conditions on bacterial and fungal biomass and carbon dioxide production in Louisiana coastal swamp forest sediment

    International Nuclear Information System (INIS)

    Seo, Dong Cheol; DeLaune, Ronald D.

    2010-01-01

    Fungal and bacterial carbon dioxide (CO 2 ) production/emission was determined under a range of redox conditions in sediment from a Louisiana swamp forest used for wastewater treatment. Sediment was incubated in microcosms at 6 Eh levels (-200, -100, 0, + 100, + 250 and + 400 mV) covering the anaerobic range found in wetland soil and sediment. Carbon dioxide production was determined by the substrate-induced respiration (SIR) inhibition method. Cycloheximide (C 15 H 23 NO 4 ) was used as the fungal inhibitor and streptomycin (C 21 H 39 N 7 O 12 ) as the bacterial inhibitor. Under moderately reducing conditions (Eh > + 250 mV), fungi contributed more than bacteria to the CO 2 production. Under highly reducing conditions (Eh ≤ 0 mV), bacteria contributed more than fungi to the total CO 2 production. The fungi/bacteria (F/B) ratios varied between 0.71-1.16 for microbial biomass C, and 0.54-0.94 for microbial biomass N. Under moderately reducing conditions (Eh ≥ + 100 mV), the F/B ratios for microbial biomass C and N were higher than that for highly reducing conditions (Eh ≤ 0 mV). In moderately reducing conditions (Eh ≥ + 100 mV), the C/N microbial biomass ratio for fungi (C/N: 13.54-14.26) was slightly higher than for bacteria (C/N: 9.61-12.07). Under highly reducing redox conditions (Eh ≤ 0 mV), the C/N microbial biomass ratio for fungi (C/N: 10.79-12.41) was higher than for bacteria (C/N: 8.21-9.14). For bacteria and fungi, the C/N microbial biomass ratios under moderately reducing conditions were higher than that in highly reducing conditions. Fungal CO 2 production from swamp forest could be of greater ecological significance under moderately reducing sediment conditions contributing to the greenhouse effect (GHE) and the global warming potential (GWP). However, increases in coastal submergence associated with global sea level rise and resultant decrease in sediment redox potential from increased flooding would likely shift CO 2 production to bacteria

  15. Redox-Magnetohydrodynamic Microfluidics Without Channels and Compatible with Electrochemical Detection Under Immunoassay Conditions

    Science.gov (United States)

    Weston, Melissa C.; Nash, Christena K.; Fritsch, Ingrid

    2010-01-01

    A unique capability of redox-magnetohydrodynamics (redox-MHD) for handling liquids on a small scale was demonstrated. A 1.2-μL solution plug was pumped from an injection site to a detector without the need for a channel to direct the flow. The redox pumping species did not interfere with enzymatic activity in a solution compatible with enzyme-linked immunoassays. Alkaline phosphatase (AP), a common enzyme label, converted p-aminophenyl phosphate (PAPP) to p-aminophenol (PAPR) in the presence of 2.5 mM Ru(NH3)6Cl2 and 2.5 mM Ru(NH3)6 Cl3, in 0.1 M Tris buffer (pH=9). A solution plug containing PAPP (no AP) was pumped through the surrounding solution containing AP (no PAPP), and the enzymatically-generated PAPR was easily detected and distinguishable electrochemically from the pumping species with square wave voltammetry down to 0.1 mM concentrations. The test device consisted of a silicon chip containing individually-addressable microband electrodes, placed on a 0.5-T NdFeB permanent magnet with the field oriented perpendicular to the chip. A 8.0-mm wide × 15.5-mm long × 1.5-mm high volume of solution was contained by a poly(dimethylsiloxane) gasket and capped with a glass slide. A steady-state fluid velocity of ~30 μm/s was generated in a reinforcing flow configuration between oppositely polarized sets of pumping electrodes with ~2.1 μA. PMID:20681513

  16. No Abrupt Changes in redox conditions associated with the end-Permian marine ecosystem collapse in the east Greenland basin

    DEFF Research Database (Denmark)

    Nielsen, Jesper K.; Shen, Y; Piasecki, Stefan

    2010-01-01

    compositions of pyrites from the East Greenland Basin. The size distributions of framboidal pyrites in sediments from a continuous section across the Permian–Triassic boundary reveal that sulfidic conditions in water columns were established about 0.7 m above the extinction event in the East Greenland Basin...... is not indicative of an abrupt change of redox chemistry in water columns, in contrast to previous claims. The integration of isotope and framboidal pyrite data provides a nearly continuous record of ocean chemistry evolution and new insights into the end-Permian extinction and delayed biotic recovery in the East...

  17. Fluctuations in the heliospheric hydrogen distribution induced by generalized and time-dependent interstellar boundary conditions

    International Nuclear Information System (INIS)

    Scherer, K.; Fahr, H.J.

    1990-01-01

    It is well known that the neutral component of the local interstellar medium (LISM) can effectively pass through the plasma interface ahead of the solar system and can penetrate deeply into the inner heliosphere. Here we present a newly-developed theoretical approach to describe the distribution function of LISM neutral hydrogen in the heliosphere, also taking into account time-dependent solar and interstellar boundary conditions. For this purpose we start from a Boltzmann-Vlasov equation, Fourier-transformed with respect to space and time coordinates, in connection with correspondingly transformed solar radiation forces and ionization rates, and then arrive at semi-analytic solutions for the transformed hydrogen velocity distribution function. As interstellar boundary conditions we allow for very general, non-Maxwellian and time-dependent distribution functions to account for the case that some LISM turbulence patterns or non-linear wave-like shock structures pass over the solar system. We consider this theoretical approach to be an ideal instrument for the synoptic interpretation of huge data samples on interplanetary Ly-α resonance glow intensities registered from different celestial directions over extended periods of time. In addition we feel that the theoretical approach presented here, when applied to interplanetary resonance glow data, may permit the detection of genuine fluctuations in the local interstellar medium. (author)

  18. The role of fluctuation-dissipation dynamics in setting initial conditions for inflation

    Science.gov (United States)

    Bastero-Gil, Mar; Berera, Arjun; Brandenberger, Robert; Moss, Ian G.; Ramos, Rudnei O.; Rosa, João G.

    2018-01-01

    We study the problem of initial conditions for slow-roll inflation along a plateau-like scalar potential within the framework of fluctuation-dissipation dynamics. We consider, in particular, that inflation was preceded by a radiation-dominated epoch where the inflaton is coupled to light degrees of freedom and may reach a near-equilibrium state. We show that the homogeneous field component can be sufficiently localized at the origin to trigger a period of slow-roll if the interactions between the inflaton and the thermal degrees of freedom are sufficiently strong and argue that this does not necessarily spoil the flatness of the potential at the quantum level. We further conclude that the inflaton can still be held at the origin after its potential begins to dominate the energy balance, leading to a period of thermal inflation. This then suppresses the effects of nonlinear interactions between the homogeneous and inhomogeneous field modes that could prevent the former from entering a slow-roll regime. Finally, we discuss the possibility of an early period of chaotic inflation, at large field values, followed by a first stage of reheating and subsequently by a second inflationary epoch along the plateau about the origin. This scenario could prevent an early overclosure of the Universe, at the same time yielding a low tensor-to-scalar ratio in agreement with observations.

  19. Phosphorus recycling and burial in Baltic Sea sediments with contrasting redox conditions

    DEFF Research Database (Denmark)

    Mort, Haydon P; Slomp, Caroline P; Gustafson, Bo G

    2010-01-01

    . Most burial of P takes place as organic P. We find no evidence for significant authigenic Ca–P formation or biogenic Ca–P burial. The lack of major inorganic P burial sinks makes the Baltic Sea very sensitive to the feedback loop between increased hypoxia, enhanced regeneration of P and increased......In this study, redox-dependent phosphorus (P) recycling and burial at 6 sites in the Baltic Sea is investigated using a combination of porewater and sediment analyses and sediment age dating (210Pb and 137Cs). We focus on sites in the Kattegat, Danish Straits and Baltic Proper where present...... be accounted for in budgets and models for the Baltic Sea....

  20. Normalized rare earth elements in water, sediments, and wine: identifying sources and environmental redox conditions

    Science.gov (United States)

    Piper, David Z.; Bau, Michael

    2013-01-01

    The concentrations of the rare earth elements (REE) in surface waters and sediments, when normalized on an element-by-element basis to one of several rock standards and plotted versus atomic number, yield curves that reveal their partitioning between different sediment fractions and the sources of those fractions, for example, between terrestrial-derived lithogenous debris and seawater-derived biogenous detritus and hydrogenous metal oxides. The REE of ancient sediments support their partitioning into these same fractions and further contribute to the identification of the redox geochemistry of the sea water in which the sediments accumulated. The normalized curves of the REE that have been examined in several South American wine varietals can be interpreted to reflect the lithology of the bedrock on which the vines may have been grown, suggesting limited fractionation during soil development.

  1. Relationship between Prevailing Redox Conditions, Water Type, Topographic Location and Methane Concentrations in Susquehanna County, NE Pennsylvania

    Science.gov (United States)

    Molofsky, L. J.; McHugh, T. E.; Connor, J. A.; Richardson, S. D.

    2014-12-01

    Historical occurrence of methane in residential water wells in parts of the Appalachian basin (Pennsylvania, New York, West Virginia) has long been recognized as a natural phenomenon. The recent increase in shale gas extraction activities in these areas has highlighted the need to distinguish between baseline methane concentrations and those that may results from gas extraction activities. For the first time, this study shows that natural dissolved methane in Northeastern Pennsylvania exhibits a relationship with prevailing redox conditions of groundwater, though this relationship is not entirely as predicted. Specifically, methane concentrations in 806 pre-drill samples from residential water wells in Susquehanna County, NE Pennsylvania, were found to be highest in samples with low SO4 concentrations but low Fe(II) concentrations. This is opposite from what would be expected if high methane concentrations were associated with a reduction of insoluble Fe(III)-minerals resulting in the release of soluble Fe(II) (and therefore, an increase in measurable dissolved iron). The water type (i.e., Na-rich vs. Ca-rich), and topographic location (i.e., valley vs. upland) was also evaluated for each of the prevailing redox states to identify associations and potential driving factors. Based on this information, this talk identifies a combination of easily identifiable natural environmental "risk" factors (i.e., advanced redox state, Na-rich water type, and valley setting) that are highly predictive of naturally elevated methane concentrations in water wells. These findings highlight simple and meaningful relationships that may be used to infer whether methane in residential water sources is natural or associated with stray gas migration.

  2. Role of biotransformation, sorption and mineralization of {sup 14}C-labelled sulfamethoxazole under different redox conditions

    Energy Technology Data Exchange (ETDEWEB)

    Alvarino, T., E-mail: teresa.alvarino@usc.es [Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Nastold, P. [Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, 40 Grundenstrasse, CH 4132 Muttenz (Switzerland); Suarez, S.; Omil, F. [Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15782 Santiago de Compostela (Spain); Corvini, P.F.X. [Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, 40 Grundenstrasse, CH 4132 Muttenz (Switzerland); State Key Laboratory for Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093 (China); Bouju, H. [Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, 40 Grundenstrasse, CH 4132 Muttenz (Switzerland)

    2016-01-15

    {sup 14}C-sulfamethoxazole biotransformation, sorption and mineralization was studied with heterotrophic and autotrophic biomass under aerobic and anoxic conditions, as well as with anaerobic biomass. The {sup 14}C-radiolabelled residues distribution in the solid, liquid and gas phases was closely monitored along a total incubation time of 190 h. Biotransformation was the main removal mechanism, mineralization and sorption remaining below 5% in all the cases, although the presence of a carbon source exerted a positive effect on the mineralization rate by the aerobic heterotrophic bacteria. In fact, an influence of the type of primary substrate and the redox potential was observed in all cases on the biotransformation and mineralization rates, since an enhancement of the removal rate was observed when an external carbon source was used as a primary substrate under aerobic conditions, while a negligible effect was observed under nitrifying conditions. In the liquid phases collected from all assays, up to three additional peaks corresponding to {sup 14}C-radiolabelled residues were detected. The highest concentration was observed under anaerobic conditions, where two radioactive metabolites were detected representing each around 15% of the total applied radioactivity after 180 h incubation. One of the metabolites detected under anoxic and anaerobic conditions, is probably resulting from ring cleavage of the isoxazole ring. - Highlights: • New procedure based on {sup 14}C to determine sulfamethoxazole (SMX) removal • Complete SMX mass balances in solid, liquid and gas phases • Quantification of SMX biotransformation, mineralization and sorption • Influence of the primary metabolism and redox potential on SMX removal • SMX metabolites have been detected and a possible chemical structure was proposed.

  3. Harmonic Injection-Based Power Fluctuation Control of Three-Phase PV Systems under Unbalanced Grid Voltage Conditions

    Directory of Open Access Journals (Sweden)

    Nian-Cheng Zhou

    2015-02-01

    Full Text Available Unbalanced voltage will inevitably cause power and DC voltage fluctuations in a three-phase PV system. The deterioration of power quality will do great harm to the PV panels and the loads, so it is necessary to suppress the power fluctuations. This paper further explores the coefficients control strategy of PV converters under unbalanced voltage conditions, aiming to suppress power fluctuations by controlling the injection of some specific orders of current harmonics into the grid. In order to achieve this, the current reference of the PV inverter has been changed by bringing in two control coefficients, and the expression of each order of the current harmonics has been deduced. Based on the standards of PV systems, the regions from which the coefficients can be selected are determined. Then, by tuning these coefficients in the feasible regions, the output parameters (power fluctuation, current THD and odd harmonics can be controlled precisely. The model of this method is built and simulated in PSCAD/EMTDC, and as a result, it is shown that the power fluctuations can be restricted according to different power quality requirements.

  4. Disentangling the record of diagenesis, local redox conditions, and global seawater chemistry during the latest Ordovician glaciation

    DEFF Research Database (Denmark)

    Ahm, Anne-Sofie Crüger; Bjerrum, Christian J.; Hammarlund, Emma U.

    2017-01-01

    The Late Ordovician stratigraphic record integrates glacio-eustatic processes, water-column redox conditions and carbon cycle dynamics. This complex stratigraphic record, however, is dominated by deposits from epeiric seas that are susceptible to local physical and chemical processes decoupled from...... the open ocean. This study contributes a unique deep water basinal perspective to the Late Ordovician (Hirnantian) glacial record and the perturbations in seawater chemistry that may have contributed to the Hirnantian mass extinction event. We analyze recently drilled cores and outcrop samples from....... The variable magnitude in the record of the Hirnantian carbon isotope excursion may be explained by this increased storage of isotopically light carbon in the deep ocean which, in combination with increased glacio-eustatic restriction, would strengthen lateral- and vertical gradients in seawater chemistry. We...

  5. Surface and redox properties of cobalt–ceria binary oxides: On the effect of Co content and pretreatment conditions

    Energy Technology Data Exchange (ETDEWEB)

    Konsolakis, Michalis, E-mail: mkonsol@science.tuc.gr [School of Production Engineering and Management, Technical University of Crete, GR-73100, Chania, Crete (Greece); Sgourakis, Michalis [School of Production Engineering and Management, Technical University of Crete, GR-73100, Chania, Crete (Greece); Carabineiro, Sónia A.C. [Laboratório de Catálise e Materiais (LCM), Laboratório Associado LSRE/LCM, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto (Portugal)

    2015-06-30

    Graphical abstract: - Highlights: • Impact of Co content and pretreatment conditions on Co/CeO{sub 2} surface chemistry. • The improved reducibility of Co/CeO{sub 2} compared to single oxides is disclosed. • A synergistic effect between Co and Ce toward more oxygen vacancies is revealed. • Calcination procedure leads to the impoverishment of catalyst surface on cobalt. • Reduction results in a uniform distribution of Co and Ce on the catalyst surface. - Abstract: Ceria-based transition metal catalysts have recently received considerable attention both in heterogeneous catalysis and electro-catalysis fields, due to their unique physicochemical characteristics. Their catalytic performance is greatly affected by the surface local chemistry and oxygen vacancies. The present study aims at investigating the impact of Co/Ce ratio and pretreatment conditions on the surface and redox properties of cobalt–ceria binary oxides. Co–ceria mixed oxides with different Co content (0, 20, 30, 60, 100 wt.%) were prepared by impregnation method and characterized by means of N{sub 2} adsorption at −196 °C, X-ray diffraction (XRD), H{sub 2} temperature-programmed reduction (H{sub 2}-TPR) and X-ray photoelectron spectroscopy (XPS). The results shown the improved reducibility of Co/CeO{sub 2} mixed oxides compared to single oxides, due to a synergistic interaction between cobalt and cerium. Oxidation pretreatment results in a preferential localization of cerium species on the outer surface. In contrast, a uniform distribution of cobalt and cerium species over the entire catalyst surface is obtained by the reduction process, which facilitates the formation of oxygen vacancies though Co{sup 3+}/Co{sup 2+} and Ce{sup 3+}/Ce{sup 4+} redox cycles. Fundamental insights toward tuning the surface chemistry of cobalt–ceria binary oxides are provided, paving the way for real-life industrial applications.

  6. Microbial C:P stoichiometry is shaped by redox conditions along an elevation gradient in humid tropical rainforests

    Science.gov (United States)

    Lin, Y.; Gross, A.; Silver, W. L.

    2017-12-01

    Elemental stoichiometry of microorganisms is intimately related to ecosystem carbon and nutrient fluxes and is ultimately controlled by the chemical (plant tissue, soil, redox) and physical (temperature, moisture, aeration) environment. Previous meta-analyses have shown that the C:P ratio of soil microbial biomass exhibits significant variations among and within biomes. Little is known about the underlying causes of this variability. We examined soil microbial C:P ratios along an elevation gradient in the Luquillo Experimental Forest in Puerto Rico. We analyzed soils from mixed forest paired with monodominant palm forest every 100 m from 300 m to 1000 m a.s.l.. Mean annual precipitation increased with increasing elevation, resulting in stronger reducing conditions and accumulation of soil Fe(II) at higher elevations. The mean value and variability of soil microbial C:P ratios generally increased with increasing elevation except at 1000 m. At high elevations (600-900 m), the average value of microbial C:P ratio (108±10:1) was significantly higher than the global average ( 55:1). We also found that soil organic P increased with increasing elevation, suggesting that an inhibition of organic P mineralization, not decreased soil P availability, may cause the high microbial C:P ratio. The soil microbial C:P ratio was positively correlated with soil HCl-extractable Fe(II), suggesting that reducing conditions may be responsible for the elevational changes observed. In a follow-up experiment, soils from mixed forests at four elevation levels (300, 500, 700, and 1000 m) were incubated under aerobic and anaerobic conditions for two weeks. We found that anaerobic incubation consistently increased the soil microbial C:P ratio relative to the aerobic incubation. Overall, our results indicate that redox conditions can shift the elemental composition of microbial biomass. The high microbial C:P ratios induced under anoxic conditions may reflect inhibition of microbial P

  7. The elemental geochemistry of Lower Triassic shallow-marine carbonates from central Saudi Arabia: Implications for redox conditions in the immediate aftermath of the latest Permian mass extinction

    Science.gov (United States)

    Eltom, Hassan A.; Abdullatif, Osman M.; Babalola, Lamidi O.

    2018-03-01

    The southern margin of the Tethys Ocean was occupied by a broad, shallow continental shelf during the Permian-Triassic boundary interval, with the area of present-day Saudi Arabia located from 10° to 30° south of the paleo-equator. The strata deposited in modern Saudi Arabia in the aftermath of the latest Permian mass extinction (LPME) are dominated by oolitic microbialite limestone (OML), which are overlain by skeletal oolitic limestones (SOL) capped by dolostones and dolomitic limestones (DDL). This succession reflects changes in depositional setting, which can be potentially tied to redox conditions using redox sensitive trace elements and rare earth elements (REEs). Statistical analyses reveals that trace elements and REEs are associated with detrital material, and possibly with diagenetic minerals as well. Proxies such as the Y/Ho, Pr/Pr*, Smn/Ybn, Lan/Smn and Lan/Ybn ratios indicate that REEs do not record a seawater-like pattern, and cannot be used as redox indicator. The presence of a normal marine fauna implies oxic conditions during deposition of the DDL and SOL units. However, the OML unit, which represents the immediate aftermath of LPME, lacks both a normal marine fauna and reliable geochemical signals, making it difficult to infer redox conditions in the depositional environment. Similar to published data from sections that reflect shallow marine condition in the LPME of the Tethys Ocean, chemical index of alteration values are consistently high throughout the study succession, suggesting globally intense chemical weathering in the aftermath of the LPME. As a result, geochemical redox proxies in shallow marine carbonates of the Tethys Ocean are likely to be contaminated by detrital material that have been generated by chemical weathering, and thus, other methods are required to determine depositional redox conditions.

  8. Photosynthetic acclimation of WS and WS-gpt2 in Arabidopsis thaliana under fluctuating natural light condition

    Science.gov (United States)

    Pa'ee, Furzani; Johnson, Giles

    2017-10-01

    Photoacclimation is a process by which photosynthetic capacity is regulated in response to environmental adjustments in terms of light regime. Photoacclimation is essential in determining the photosynthetic capacity to optimize light use and to avoid potentially damaging effects. Previous work in our laboratory has identified a gene, gpt2 (At1g61800) that is essential for plants to acclimate to an increase and decrease of growth irradiance, separately. To investigate the photoacclimation ability towards fluctuating natural light condition in Arabidopsis thaliana, photosynthetic capacity was measured in plants of the accession Wassileskija (WS) and in plants lacking expression of the gene At1g61800 (WS-gpt2). The experiment was carried out over a time span from early Autumn to early Spring season in 2010-2011 and 2011-2012. The seedlings were grown in an unheated greenhouse in Manchester, UK without supplementary lighting. Gas exchange measurements and chlorophyll content estimation were performed on WS and WS-gpt2 and it showed that both sets of plants were able to acclimate to fluctuating natural light condition. Therefore, it is suggested that the mechanisms of acclimation in a separate growth light condition is mechanistically distinct than the mechanism under fluctuating natural light condition.

  9. Hydrologic influence on redox dynamics in estuarine environments

    Science.gov (United States)

    Michael, H. A.; Kim, K. H.; Guimond, J. A.; Heiss, J.; Ullman, W. J.; Seyfferth, A.

    2017-12-01

    Redox conditions in coastal aquifers control reactions that impact nutrient cycling, contaminant release, and carbon budgets, with implications for water resources and ecosystem health. Hydrologic changes can shift redox boundaries and inputs of reactants, especially in dynamic coastal systems subject to fluctuations on tidal, lunar, and longer timescales. We present two examples of redox shifts in estuarine systems in Delaware, USA: a beach aquifer and a saltmarsh. Beach aquifers are biogeochemical hot spots due to mixing between fresh groundwater and infiltrating seawater. At Cape Henlopen, DE, geochemical measurements identified reactions in the intertidal aquifer that include cycling of carbon, nitrogen, iron, and sulfur. Measurements and modeling illustrate that redox potential as well as the locations of redox reactions shift on tidal to seasonal timescales and in response to changing beach and aquifer properties, impacting overall rates of reactions such as denitrification that reduces N loads to coastal waters. In the St. Jones National Estuarine Research Reserve, tidal fluctuations in channels cause periodic groundwater-surface water exchange, water table movement, and intermittent flooding that varies spatially across the saltmarsh. These changes create shifts in redox potential that are greatest near channels and in the top 20 cm of sediments. The magnitude of redox change depends on hydrologic setting (near channels or in marsh interior), hydrologic conditions (tidal stage, seasonal shifts), as well as prevalence of macropores created by crab burrows that change seasonally with crab activity. These shifts correspond to changes in porewater chemistry that have implications for nutrient cycling and carbon export to the ocean. Understanding hydrologic influence on redox geochemistry is critical for predicting how these systems and their ecosystem services may change in the future in response to anthropogenic and climate change.

  10. DC-Voltage Fluctuation Elimination Through a DC-Capacitor Current Control for DFIG Converters Under Unbalanced Grid Voltage Conditions

    DEFF Research Database (Denmark)

    Liu, Changjin; Xu, Dehong; Zhu, Nan

    2013-01-01

    Unbalanced grid voltage causes a large second-order harmonic current in the dc-link capacitors as well as dc-voltage fluctuation, which potentially will degrade the lifespan and reliability of the capacitors in voltage source converters. This paper proposes a novel dc-capacitor current control me...... grid voltage conditions. A modular implementation method of the proposed control strategy is developed for the DFIG controller. Finally, experiments are presented to validate the theoretical analysis....

  11. Probing freeze-out conditions in heavy ion collisions with moments of charge fluctuations

    CERN Document Server

    Karsch, Frithjof

    2011-01-01

    We calculate the first four moments of baryon number, electric charge and strangeness fluctuations within the hadron resonance gas model. Different moments and their ratios as well as skewness and kurtosis are evaluated on the phenomenologically determined freeze-out curve in the temperature, baryon chemical potential plane. The model results and its predictions as well as relations between different moments are compared with the first data on net proton fluctuations in Au-Au collisions obtained at RHIC by the STAR Collaboration. We find good agreement between the model calculations and experimental results. We also point out that higher order moments should be more sensitive to critical behavior and will also distinguish hadron resonance gas model calculations from results obtained from lattice QCD.

  12. Disentangling the record of diagenesis, local redox conditions, and global seawater chemistry during the latest Ordovician glaciation

    Science.gov (United States)

    Ahm, Anne-Sofie C.; Bjerrum, Christian J.; Hammarlund, Emma U.

    2017-02-01

    The Late Ordovician stratigraphic record integrates glacio-eustatic processes, water-column redox conditions and carbon cycle dynamics. This complex stratigraphic record, however, is dominated by deposits from epeiric seas that are susceptible to local physical and chemical processes decoupled from the open ocean. This study contributes a unique deep water basinal perspective to the Late Ordovician (Hirnantian) glacial record and the perturbations in seawater chemistry that may have contributed to the Hirnantian mass extinction event. We analyze recently drilled cores and outcrop samples from the upper Vinini Formation in central Nevada and report combined trace- and major element geochemistry, Fe speciation (FePy /FeHR and FeHR /FeT), and stable isotope chemostratigraphy (δ13COrg and δ34SPy). Measurements of paired samples from outcrop and core reveal that reactive Fe is preserved mainly as pyrite in core samples, while outcrop samples have been significantly altered as pyrite has been oxidized and remobilized by modern weathering processes. Fe speciation in the more pristine core samples indicates persistent deep water anoxia, at least locally through the Late Ordovician, in contrast to the prevailing interpretation of increased Hirnantian water column oxygenation in shallower environments. Deep water redox conditions were likely decoupled from shallower environments by a basinal shift in organic matter export driven by decreasing rates of organic matter degradation and decreasing shelf areas. The variable magnitude in the record of the Hirnantian carbon isotope excursion may be explained by this increased storage of isotopically light carbon in the deep ocean which, in combination with increased glacio-eustatic restriction, would strengthen lateral- and vertical gradients in seawater chemistry. We adopt multivariate statistical methods to deconstruct the spatial and temporal re-organization of seawater chemistry during the Hirnantian glaciation and attempt to

  13. Influence of redox conditions on the intensity of Mars crustal magnetic anomalies

    Science.gov (United States)

    Brachfeld, Stefanie; Shah, Deepa; First, Emily; Hammer, Julia; Bowles, Julie

    2015-10-01

    We evaluate the relationship between the intensity of remanent magnetization and fO2 in natural and synthetic Mars meteorites. The olivine-phyric shergottite meteorite Yamato 980459 (Y-980459) and a sulfur-free synthetic analog (Y-98*) of identical major element composition were analyzed to explore the rock magnetic and remanence properties of a basalt crystallized from a primitive melt, and to explore the role of magmatic and alteration environment fO2 on Mars crustal anomalies. The reducing conditions under which Y-980459 is estimated to have formed (QFM-2.5; Shearer et al. 2006) were replicated during the synthesis of Y-98*. Y-980459 contains pyrrhotite and chromite. Chromite is the only magnetic phase in Y-98*. The remanence-carrying capacity of Y-980459 is comparable to other shergottites that formed in the fO2 range of QFM-3 to QFM-1. The remanence-carrying capacity of these low fO2 basalts is 1-2 orders of magnitude too weak to account for the intense crustal anomalies observed in Mars's southern cratered highlands. Moderately oxidizing conditions of >QFM-1, which are more commonly observed in nakhlites and Noachian breccias, are key to generating either a primary igneous assemblage or secondary alteration assemblage capable of acquiring an intense remanent magnetization, regardless of the basalt character or thermal history. This suggests that if igneous rocks are responsible for the intensely magnetized crust, these oxidizing conditions must have existed in the magmatic plumbing systems of early Mars or must have existed in the crust during secondary processes that led to acquisition of a chemical remanent magnetization.

  14. Modeling the Lag Period and Exponential Growth of Listeria monocytogenes under Conditions of Fluctuating Temperature and Water Activity Values▿

    Science.gov (United States)

    Muñoz-Cuevas, Marina; Fernández, Pablo S.; George, Susan; Pin, Carmen

    2010-01-01

    The dynamic model for the growth of a bacterial population described by Baranyi and Roberts (J. Baranyi and T. A. Roberts, Int. J. Food Microbiol. 23:277-294, 1994) was applied to model the lag period and exponential growth of Listeria monocytogenes under conditions of fluctuating temperature and water activity (aw) values. To model the duration of the lag phase, the dependence of the parameter h0, which quantifies the amount of work done during the lag period, on the previous and current environmental conditions was determined experimentally. This parameter depended not only on the magnitude of the change between the previous and current environmental conditions but also on the current growth conditions. In an exponentially growing population, any change in the environment requiring a certain amount of work to adapt to the new conditions initiated a lag period that lasted until that work was finished. Observations for several scenarios in which exponential growth was halted by a sudden change in the temperature and/or aw were in good agreement with predictions. When a population already in a lag period was subjected to environmental fluctuations, the system was reset with a new lag phase. The work to be done during the new lag phase was estimated to be the workload due to the environmental change plus the unfinished workload from the uncompleted previous lag phase. PMID:20208022

  15. Modeling the lag period and exponential growth of Listeria monocytogenes under conditions of fluctuating temperature and water activity values.

    Science.gov (United States)

    Muñoz-Cuevas, Marina; Fernández, Pablo S; George, Susan; Pin, Carmen

    2010-05-01

    The dynamic model for the growth of a bacterial population described by Baranyi and Roberts (J. Baranyi and T. A. Roberts, Int. J. Food Microbiol. 23:277-294, 1994) was applied to model the lag period and exponential growth of Listeria monocytogenes under conditions of fluctuating temperature and water activity (a(w)) values. To model the duration of the lag phase, the dependence of the parameter h(0), which quantifies the amount of work done during the lag period, on the previous and current environmental conditions was determined experimentally. This parameter depended not only on the magnitude of the change between the previous and current environmental conditions but also on the current growth conditions. In an exponentially growing population, any change in the environment requiring a certain amount of work to adapt to the new conditions initiated a lag period that lasted until that work was finished. Observations for several scenarios in which exponential growth was halted by a sudden change in the temperature and/or a(w) were in good agreement with predictions. When a population already in a lag period was subjected to environmental fluctuations, the system was reset with a new lag phase. The work to be done during the new lag phase was estimated to be the workload due to the environmental change plus the unfinished workload from the uncompleted previous lag phase.

  16. Biofilm-induced bioclogging produces sharp interfaces in hyporheic flow, redox conditions, and microbial community structure

    Science.gov (United States)

    Caruso, Alice; Boano, Fulvio; Ridolfi, Luca; Chopp, David L.; Packman, Aaron

    2017-05-01

    Riverbed sediments host important biogeochemical processes that play a key role in nutrient dynamics. Sedimentary nutrient transformations are mediated by bacteria in the form of attached biofilms. The influence of microbial metabolic activity on the hydrochemical conditions within the hyporheic zone is poorly understood. We present a hydrobiogeochemical model to assess how the growth of heterotrophic and autotrophic biomass affects the transport and transformation of dissolved nitrogen compounds in bed form-induced hyporheic zones. Coupling between hyporheic exchange, nitrogen metabolism, and biomass growth leads to an equilibrium between permeability reduction and microbial metabolism that yields shallow hyporheic flows in a region with low permeability and high rates of microbial metabolism near the stream-sediment interface. The results show that the bioclogging caused by microbial growth can constrain rates and patterns of hyporheic fluxes and microbial transformation rate in many streams.

  17. Anaerobic biodegradation of gasoline oxygenates. Extrapolation of information to multiple sites and redox conditions

    Energy Technology Data Exchange (ETDEWEB)

    Mormile, M.R.; Liu, S.; Suflita, J.M. (Univ. of Oklahoma, Norman, OK (United States))

    1994-09-01

    A series of alcohol, ketone, ester, and ether oxygenates were tested for their susceptibility to anaerobic decay in samples from four chronically contaminated sedimentary environments. The effect of various electron acceptors on oxygenate biodegradation was also evaluated with a single inoculum source. In addition, two acetogenic bacteria were tested for their ability to metabolize selected oxygenate compounds. The susceptibility of the test oxygenates to anaerobic decay could be related to their chemical structure. That is, compounds other than the ethers that possessed primary or secondary substituted carbon atoms were readily degraded under all conditions tested while compounds that had tertiary substituted carbon atoms resisted biodegradation. The ether oxygenates were generally not degraded when incubated with various inocula, regardless of the electron acceptor status. The exceptions included methyl butyl ether, which was depleted in both sulfate-reducing and methanogenic incubations, and the partial transformation of methyl tert-butyl ether to tert-butanol after a 152-day acclimation period in a single replicate from a river sediment chronically contaminated with fuel. Heat-inactivated control incubations suggested that the latter transformation was biologically catalyzed. 47 refs., 1 fig., 3 tabs.

  18. Water Quality and Sediment Profile in Shrimp Culture with Different Sediment Redox Potential and Stocking Densities Under Laboratory Condition

    Directory of Open Access Journals (Sweden)

    Wiyoto Wiyoto

    2016-06-01

    Full Text Available Sediment quality has been considered as one of the prime factors influencing the environment quality that support maximum shrimp production.The aim of the study was toevaluate the effects of sediment redox potential and shrimp stocking density on the profile of some sediment and water quality parameters. Two factors randomized factorial design was applied, with stocking density (60 and 120 shrimps.m-2 as the first variable and sediment redox potential (-65 mV, -108 mV and -206 mV as the second variable. Some significant changes in TP, total Mn, and total S concentrations in the sediment were observed after the experimentation (P<0.05. Sediment redox potential significantly affected the dissolved oxygen, TAN, NO2, NO3, and H2S concentrations in the water. Whereas shrimp stocking density affected all water quality parameters except H2S concentration. Significant interactions between redox potential and stocking densities were observed in the nitrite and alkalinity concentrations. The significant effects of both shrimp density and redox potential on the sediment and water parameters in particular those that are known to directly affect the shrimp welfare (e.g. oxygen, ammonia, nitrite and H2S indicate that these variables are of important aspects in shrimp pond management. Furthermore, the results clearly showed that -206mV redox potential significantly reduced the dissolved oxygen concentration in the sediment-water interface and increased the generation of H2S in water column. Thereby, this redox potential level is not advisable for shrimp culture system. Keywords: redox potential, stocking density.

  19. Mercury transformations in resuspended contaminated sediment controlled by redox conditions, chemical speciation and sources of organic matter

    Science.gov (United States)

    Zhu, Wei; Song, Yu; Adediran, Gbotemi A.; Jiang, Tao; Reis, Ana T.; Pereira, Eduarda; Skyllberg, Ulf; Björn, Erik

    2018-01-01

    Mercury (Hg) contaminated sediments can be significant sources of Hg in aquatic ecosystems and, through re-emission processes, to the atmosphere. Transformation and release of Hg may be enhanced by various sediment perturbation processes, and controlling biogeochemical factors largely remain unclear. We investigated how rates of Hg transformations in pulp-fiber enriched sediment contaminated by Hg from chlor-alkali industry were controlled by (i) transient redox-changes in sulfur and iron chemistry, (ii) the chemical speciation and solubility of Hg, and (iii) the sources and characteristics of organic matter (OM). Sediment-bottom water microcosm systems were exposed to four combinations of air and nitrogen gas for a total time of 24 h. The treatments were: 24 h N2, 0.5 h air + 23.5 h N2, 4 h air + 20 h N2 and 24 h of air exposure. As a result of these treatments, microcosms spanned a wide range of redox potential, as reflected by the dissolved sulfide concentration range of ≤0.3-97 μM. Four different chemical species of inorganic divalent Hg (HgII) and methyl mercury (MeHg), enriched in different Hg isotope tracers, were added to the microcosms: 201Hg(NO3)2(aq), 202HgII adsorbed to OM (202HgII-OM(ads)), 198HgII as microcrystalline metacinnabar (β-198HgS(s)) and Me204HgCl(aq). Microcosm systems were composed of bottom water mixed with sediment taken at 0-2, 0-5 and 0-10 cm depth intervals. The composition of OM varied with sediment depth such that compared to deeper sediment, the 0-2 cm depth-interval had a 2-fold higher contribution of labile OM originating from algal and terrestrial inputs, serving as metabolic electron-donors for microorganisms. The potential methylation rate constant (kmeth) of Hg tracers and net formation of ambient MeHg (MeHg/THg molar ratio) increased up to 50% and 400%, respectively at intermediate oxidative conditions, likely because of an observed 2-fold increase in sulfate concentration stimulating the activity of sulfate reducing

  20. Redox Buffer Strength

    Science.gov (United States)

    de Levie, Robert

    1999-04-01

    The proper functioning of enzymes in bodily fluids requires that the pH be maintained within rather narrow limits. The first line of defense against large pH fluctuations in such fluids is the passive control provided by the presence of pH buffers. The ability of pH buffers to stabilize the pH is indicated by the buffer value b introduced in 1922 by van Slyke. It is equally important for many enzymes that the redox potential is kept within a narrow range. In that case, stability of the potential is most readily achieved with a redox buffer. In this communication we define the redox buffer strength by analogy with acid-base buffer strength.

  1. Earthworm responses to Cd and Cu under fluctuating environmental conditions: a comparison with results from laboratory exposures

    Energy Technology Data Exchange (ETDEWEB)

    Spurgeon, David J. [Centre for Ecology and Hydrology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire, PE28 2LS (United Kingdom)]. E-mail: dasp@ceh.ac.uk; Svendsen, Claus [Centre for Ecology and Hydrology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire, PE28 2LS (United Kingdom)]. E-mail: csv@ceh.ac.uk; Lister, Lindsay J. [Centre for Ecology and Hydrology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire, PE28 2LS (United Kingdom)]. E-mail: llist@ceh.ac.uk; Hankard, Peter K. [Centre for Ecology and Hydrology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire, PE28 2LS (United Kingdom)]. E-mail: pkh@ceh.ac.uk; Kille, Peter [School of Biosciences, University of Cardiff, PO Box 911, Cardiff, CF1 3US (United Kingdom)]. E-mail: kille@cardiff.ac.uk

    2005-08-15

    Laboratory toxicity tests are usually conducted under stable ambient conditions, while exposures in ecosystems occur in a fluctuating climate. To assess how climate influences the toxicity of Cu and Cd for the earthworm Lumbricus rubellus, this study compared effects for life-cycle parameters (survival, reproduction), cellular status (lysosomal membrane stability), gene expression (transcript of the metal binding protein metallothionein-2) and tissue metal concentration measured under outdoor conditions, with the same responses under constant conditions as measured by Spurgeon et al. [Spurgeon, D.J., Svendsen, C., Weeks, J.M., Hankard, P.K., Stubberud, H.E., Kammenga, J.E., 2003. Quantifying copper and cadmium impacts on intrinsic rate of population increase in the terrestrial oligochaete Lumbricus rubellus. Environmental Toxicology and Chemistry 22, 1465-1472]. Both metals were found to significantly influence earthworm reproduction, compromise lysosomal membrane stability and induce MT-2 gene expression in the outdoor system. Comparison with physiological and life-cycle responses in the laboratory indicated similar response patterns and effect concentrations for Cu. For Cd, lysosomal membrane stability and MT-2expression showed comparable responses in both exposures. Juvenile production rate, however, gave different dose response relationships, with the EC-{sub 50} in the outdoor test approximately half that in the laboratory test. A difference in Cd accumulation was also seen. Overall, however, the comparison indicated only a marginal effect of environmental fluctuations typical for northern temperate Europe on earthworm sensitivity to the two metals. - Comparative analysis of life-cycle, physiological and molecular responses to Cu and Cd indicate similar responses under static and fluctuating climate regimes.

  2. Plasma structure change and intermittent fluctuation near magnetic island X-point under detached plasma condition in LHD

    International Nuclear Information System (INIS)

    Ohno, N.; Tsuji, Y.; Tanaka, H.; Masuzaki, S.; Kobayashi, M.; Akiyama, T.; Morisaki, T.; Motojima, G.; Narushima, Y.

    2014-10-01

    Plasma profiles and intermittent fluctuations near the helical divertor X-point and on a divertor plate were investigated using a fast scanning Langmuir probe and a probe array embedded on a divertor plate in detached divertor condition that was sustained by applying a resonant magnetic perturbation (RMP) field in LHD. When the RMP induced magnetic island X-point (n/m = 1/1) is located near the helical divertor X-point, the reduction of particle flux accompanied by the plasma detachment occurred near the helical divertor X-point (n/m = 2/10), which leads to the reduction of the particle flux at the strike point on the divertor plate. We also found that when the divertor plasma turned to be the detached condition, the enhanced plasma fluctuations were confirmed between the helical divertor X-point and ergodic region, which exhibited a dynamic behavior having a large amount of positive-spike components with highly intermittent property. (author)

  3. Trace and rare earth elemental geochemistry of carbonate succession in the Middle Gaoyuzhuang Formation, Pingquan Section: Implications for Early Mesoproterozoic ocean redox conditions

    Directory of Open Access Journals (Sweden)

    Guo Hua

    2013-04-01

    Full Text Available The concentrations of redox-sensitive trace elements, such as uranium (U, vanadium (V, molybdenum (Mo, cobalt (Co, chromium (Cr and rare earth elements (REE+Y were determined in a given carbonate succession in the Gaoyuzhuang Formation (~1.56 Ga, which spans depths from outer shelf to intertidal, to explore the Early Mesoproterozoic ocean redox conditions. The values of the Zr-normalized redox-sensitive trace element concentrations and some relevant ratios show obvious changes from bottom to top in the succession. Samples from the outer shelf setting (M1 interval demonstrate significantly enhanced values in Zr-normalized redox-sensitive trace element concentrations and relevant ratios (the peaks of Mo/U, V/Cr and Ni/Co ratios larger than 8, 4.25, and 7, respectively. Authigenic framboidal pyrites were also found within oncolite-like carbonate concretions and surrounding host rocks in this interval. These all indicate a euxinic state in the outer shelf environment. Less enrichment of Zr-normalized redox-sensitive elemental abundances and a mild decrease in the values of geochemical ratios were present in the inner shelf environment (M2 interval (the V/Cr and Ni/Co ratios fall into a range of 2.5-4.25 and 4-5, respectively, suggesting dysoxic conditions dominant in the inner shelf setting. Samples from the shallower subtidal and intertidal settings (M3 and M4 intervals are mostly invariable with much lower values of Zr-normalized redox-sensitive elements and relevant ratios, with the V/Cr and Ni/Co ratios typically near or less than 2 and 5 respectively, indicative of oxic conditions in the high-energy subtidal/intertidal zones. A remarkable negative Ce anomaly exhibited in the shale-normalized REE+Y diagram in the M4 interval may provide evidence in support of the hypothesis. Taken together, our results suggest a relatively shallow chemocline in the Early Mesoproterozoic ocean: the transitions between euxinic, dysoxic and oxic may occur in quiet

  4. Fluctuating water depths affect American alligator (Alligator mississippiensis) body condition in the Everglades, Florida, USA

    Science.gov (United States)

    Brandt, Laura A.; Beauchamp, Jeffrey S.; Jeffery, Brian M.; Cherkiss, Michael S.; Mazzotti, Frank J.

    2016-01-01

    Successful restoration of wetland ecosystems requires knowledge of wetland hydrologic patterns and an understanding of how those patterns affect wetland plant and animal populations.Within the Everglades, Florida, USA restoration, an applied science strategy including conceptual ecological models linking drivers to indicators is being used to organize current scientific understanding to support restoration efforts. A key driver of the ecosystem affecting the distribution and abundance of organisms is the timing, distribution, and volume of water flows that result in water depth patterns across the landscape. American alligators (Alligator mississippiensis) are one of the ecological indicators being used to assess Everglades restoration because they are a keystone species and integrate biological impacts of hydrological operations through all life stages. Alligator body condition (the relative fatness of an animal) is one of the metrics being used and targets have been set to allow us to track progress. We examined trends in alligator body condition using Fulton’s K over a 15 year period (2000–2014) at seven different wetland areas within the Everglades ecosystem, assessed patterns and trends relative to restoration targets, and related those trends to hydrologic variables. We developed a series of 17 a priori hypotheses that we tested with an information theoretic approach to identify which hydrologic factors affect alligator body condition. Alligator body condition was highest throughout the Everglades during the early 2000s and is approximately 5–10% lower now (2014). Values have varied by year, area, and hydrology. Body condition was positively correlated with range in water depth and fall water depth. Our top model was the “Current” model and included variables that describe current year hydrology (spring depth, fall depth, hydroperiod, range, interaction of range and fall depth, interaction of range and hydroperiod). Across all models, interaction

  5. DOC-dynamics in a small headwater catchment as driven by redox fluctuations and hydrological flow paths - are DOC exports mediated by iron reduction/oxidation cycles?

    Science.gov (United States)

    Knorr, K.-H.

    2013-02-01

    Dissolved organic carbon (DOC) exports from many catchments in Europe and North-America are steadily increasing. Several studies have sought to explain this observation. As possible causes, a decrease in acid rain or sulfate deposition, concomitant reductions in ionic strength and increasing temperatures were identified. DOC often originates from riparian wetlands; but here, despite higher DOC concentrations, ionic strength in pore waters usually exceeds that in surface waters. In the catchment under study, DOC concentrations were synchronous with dissolved iron concentrations in pore and stream water. This study aims at testing the hypothesis that DOC exports are mediated by iron reduction/oxidation cycles. Following the observed hydrographs, δ18O of water and DOC fluorescence, the wetlands were identified as the main source of DOC. Antecedent biogeochemical conditions, i.e., water table levels in the wetlands, influenced the discharge patterns of nitrate, iron and DOC during an event. The correlation of DOC with pH was positive in pore waters, but negative in surface waters; it was negative for DOC with sulfate in pore waters, but only weak in surface waters. Though, the positive correlation of DOC with iron was universal for pore and surface water. The decline of DOC and iron concentrations in transition from anoxic wetland pore water to oxic stream water suggests a flocculation of DOC with oxidising iron, leading to a drop in pH in the stream during high DOC fluxes. The pore water did not per se differ in pH. There is, thus, a need to consider processes more thoroughly of DOC mobilisation in wetlands when interpreting DOC exports from catchments. The coupling of DOC with iron fluxes suggested that increased DOC exports could at least, in part, be caused by increasing activities in iron reduction, possibly due to increases in temperature, increasing wetness of riparian wetlands, or by a shift from sulfate dominated to iron reduction dominated biogeochemical

  6. DOC-dynamics in a small headwater catchment as driven by redox fluctuations and hydrological flow paths – are DOC exports mediated by iron reduction/oxidation cycles?

    Directory of Open Access Journals (Sweden)

    K.-H. Knorr

    2013-02-01

    Full Text Available Dissolved organic carbon (DOC exports from many catchments in Europe and North-America are steadily increasing. Several studies have sought to explain this observation. As possible causes, a decrease in acid rain or sulfate deposition, concomitant reductions in ionic strength and increasing temperatures were identified. DOC often originates from riparian wetlands; but here, despite higher DOC concentrations, ionic strength in pore waters usually exceeds that in surface waters. In the catchment under study, DOC concentrations were synchronous with dissolved iron concentrations in pore and stream water. This study aims at testing the hypothesis that DOC exports are mediated by iron reduction/oxidation cycles. Following the observed hydrographs, δ18O of water and DOC fluorescence, the wetlands were identified as the main source of DOC. Antecedent biogeochemical conditions, i.e., water table levels in the wetlands, influenced the discharge patterns of nitrate, iron and DOC during an event. The correlation of DOC with pH was positive in pore waters, but negative in surface waters; it was negative for DOC with sulfate in pore waters, but only weak in surface waters. Though, the positive correlation of DOC with iron was universal for pore and surface water. The decline of DOC and iron concentrations in transition from anoxic wetland pore water to oxic stream water suggests a flocculation of DOC with oxidising iron, leading to a drop in pH in the stream during high DOC fluxes. The pore water did not per se differ in pH. There is, thus, a need to consider processes more thoroughly of DOC mobilisation in wetlands when interpreting DOC exports from catchments. The coupling of DOC with iron fluxes suggested that increased DOC exports could at least, in part, be caused by increasing activities in iron reduction, possibly due to increases in temperature, increasing wetness of riparian wetlands, or by a shift from sulfate dominated to iron

  7. Sliding-window analysis tracks fluctuations in amygdala functional connectivity associated with physiological arousal and vigilance during fear conditioning.

    Science.gov (United States)

    Baczkowski, Blazej M; Johnstone, Tom; Walter, Henrik; Erk, Susanne; Veer, Ilya M

    2017-06-01

    We evaluated whether sliding-window analysis can reveal functionally relevant brain network dynamics during a well-established fear conditioning paradigm. To this end, we tested if fMRI fluctuations in amygdala functional connectivity (FC) can be related to task-induced changes in physiological arousal and vigilance, as reflected in the skin conductance level (SCL). Thirty-two healthy individuals participated in the study. For the sliding-window analysis we used windows that were shifted by one volume at a time. Amygdala FC was calculated for each of these windows. Simultaneously acquired SCL time series were averaged over time frames that corresponded to the sliding-window FC analysis, which were subsequently regressed against the whole-brain seed-based amygdala sliding-window FC using the GLM. Surrogate time series were generated to test whether connectivity dynamics could have occurred by chance. In addition, results were contrasted against static amygdala FC and sliding-window FC of the primary visual cortex, which was chosen as a control seed, while a physio-physiological interaction (PPI) was performed as cross-validation. During periods of increased SCL, the left amygdala became more strongly coupled with the bilateral insula and anterior cingulate cortex, core areas of the salience network. The sliding-window analysis yielded a connectivity pattern that was unlikely to have occurred by chance, was spatially distinct from static amygdala FC and from sliding-window FC of the primary visual cortex, but was highly comparable to that of the PPI analysis. We conclude that sliding-window analysis can reveal functionally relevant fluctuations in connectivity in the context of an externally cued task. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Sulphate chemistry under pressurized oxidizing, reducing and fluctuating conditions; Sulfatkemi under trycksatta oxiderande, reducerande och fluktuerande foerhaallanden

    Energy Technology Data Exchange (ETDEWEB)

    Hupa, M.; Yrjas, P.; Backman, P. [Aabo Akademi, Turku (Finland). Combustion Chemistry Research Group

    1997-10-01

    In the literature it has been reported that sulfur capture with limestone (CaCO{sub 3}) under atmospheric fluidized bed combustion conditions reaches a maximum at about 850 deg C. Previously, the maximum has been attributed to the sintering of sorbent particles which decreases the reactive surface area. Lately, also another explanation has been reported. In this case the sulfur capture decrease at higher temperatures was concluded to be due to fluctuating oxidizing/reducing conditions in the atmospheric combustor. In this work the influence of alternating oxidizing/reducing conditions on SO{sub 2} capture at atmospheric and elevated pressure (15 bar) has been studied. In the pressurized case, the CO{sub 2} partial pressure was kept high enough to prevent CaCO{sub 3} from calcining and therefore the CaSO{sub 4} would not form CaO but CaCO{sub 3} under reducing conditions. The experiments were done with a pressurized TGA by periodically changing the gas environment between oxidizing (O{sub 2}. SO{sub 2}, CO{sub 2} and N{sub 2}) and slightly reducing (CO, SO{sub 2}, CO{sub 2} and N{sub 2}) gas mixtures at different temperatures. The results from the experiments showed that under normal pressure and slightly reducing conditions CaO formation from CaSO{sub 4} increased with temperature as expected. However, no significant amounts of CaCO{sub 3} were formed from CaSO{sub 4} at elevated pressure. It was also concluded that since the formation of CaO from CaSO{sub 4} was relatively slow it could not explain the sharp sulfur capture maximum at about 850 deg C. Therefore, it was assumed that the strongly reducing zones, where CaS thermodynamically is the stable compound, play a more important role concerning the sulfur capture in fluidized bed combustors. (orig.)

  9. Fluctuations of conserved charges within a Hadron Resonance Gas approach: chemical freeze-out conditions from net-charge and net-proton fluctuations

    Directory of Open Access Journals (Sweden)

    Mantovani Sarti V.

    2015-01-01

    Full Text Available We present an analysis of fluctuations of conserved charges, as baryon number B and electric charge Q, using the Hadron Resonance Gas (HRG model. The study of such observables within a theoretical approach and the comparison to experimental data on the moments of the multiplicity distributions of specific particles represents a useful tool to extract the chemical freeze-out parameters, temperature T and baryo-chemical potential μB. In particular we calculate ratios of higher-order susceptibilities for net-protons and net-charge and we compare our results to the latest data from STAR collaboration. The effect of resonance decays and kinematic cuts applied in the experimental analysis in transverse momentum, rapidity and pseudo-rapidity are included in the calculation along with the randomization of nucleons occurring in the hadronic phase.

  10. Redox fluctuations in the Early Ordovician oceans

    DEFF Research Database (Denmark)

    D'Arcy, Joan Mary; Gilleaudeau, Geoffrey Jon; Peralta, Silvio

    2017-01-01

    δ53Cr values of limestones and dolostones from an Early Ordovician marine carbonate platform (Cerro La Silla section of the Precordillera, San Juan, Argentina). An increasing number of studies support the hypothesis that the Early Ordovician oceans were stratified with respect to oxygen...

  11. Health Effects of Short-Term Fluctuations in Macroeconomic Conditions: The Case of Hypertension for Older Americans.

    Science.gov (United States)

    Angrisani, Marco; Lee, Jinkook

    2016-11-01

    We investigate the health effects of short-term macroeconomic fluctuations as described by changes in unemployment rate, house, and stock market price indexes. The 'Great Recession' provides the opportunity to conduct this analysis as it involved contemporaneous shocks to the labor, housing, and stock markets. Using panel data from the Health and Retirement Study over the period 2004-2010, we relate changes in hypertension status to changes in state-level unemployment rate and house prices and to changes in stock market prices. We consider hypertension, a disease related to stress and of high prevalence among older adults, that has received little attention in the literature linking macroeconomic conditions to individual health. Our analysis exploits self-reports of hypertension diagnosis as well as directly measured blood pressure readings. Using both measures, we find that the likelihood of developing hypertension is negatively related to changes in house prices. Also, decreasing house prices lower the probability of stopping hypertension medication treatment for individuals previously diagnosed with the condition. We do not observe significant associations between hypertension and either changes in unemployment rate or stock market prices. We document heterogeneity in the estimated health effects of the recession by gender, education, asset ownership, and work status. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  12. Crystal chemistry of iron in low-temperature chlorites, implications for geo-thermometry and the determination of redox paleo-conditions in uranium deposits

    International Nuclear Information System (INIS)

    Rigault, Cecile

    2010-01-01

    In contexts of uranium deposits, redox conditions constitute the main factor controlling the uranium deposition. Often observed in these deposits, chlorites are the unique clay mineral which can be able to record in their structure the redox conditions through their Fe 3+ /SFe ratio. However, the common presence of several populations of chlorites makes difficult to understand the message carried out by these minerals. Thanks to μ-XANES spectroscopy, we are now able to measure on thin sections the Fe 3+ /SFe ratio in chlorites with an accuracy of 5 %. Measurements show that it can reach 60 % in di-tri-octahedral chlorites and 5 % to more than 40 % for tri-octahedral chlorites. In hydrothermal contexts where chlorites crystallize through a dissolution-recrystallization process, their Fe 3+ /SFe ratio decreases with the increase of the global Fe content. Diagenetic chlorites observed resulting from the polymorphic transformation of berthierine have a different behavior because there is no link between their total iron content and their Fe 3+ /SFe ratio: their chemistry is directly inherited from the one of the precursor mineral because this transformation does not allow a reorganization of cations in the structure. This transformation explains that thermodynamic models cannot work for these phases. For the use of chlorites as makers of redox paleo-conditions in contexts of uranium deposits where diagenetic and hydrothermal chlorites can be present, it is decisive to determine their origin, for example analyzing their polytype: Ib (b=90 degrees) for chlorites crystallized from solid-state transformation and IIb for chlorites crystallized through dissolution-recrystallization process. (author)

  13. Impact of Redox Condition on Fractionation and Bioaccessibility of Arsenic in Arsenic-Contaminated Soils Remediated by Iron Amendments: A Long-Term Experiment

    Directory of Open Access Journals (Sweden)

    Quan Zhang

    2018-01-01

    Full Text Available Iron-bearing amendments, such as iron grit, are proved to be effective amendments for the remediation of arsenic- (As- contaminated soils. In present study, the effect of redox condition on As fractions in As-contaminated soils remediated by iron grit was investigated, and the bioaccessibility of As in soils under anoxic condition was evaluated. Results showed that the labile fractions of As in soils decreased significantly after the addition of iron grit, while the unlabile fractions of As increased rapidly, and the bioaccessibility of As was negligible after 180 d incubation. More labile fractions of As in iron-amended soils were transformed into less mobilizable or unlabile fractions with the contact time. Correspondingly, the bioaccessibility of As in iron-amended soils under the aerobic condition was lower than that under the anoxic condition after 180 d incubation. The redistribution of loosely adsorbed fraction of As in soils occurred under the anoxic condition, which is likely ascribed to the reduction of As(V to As(III and the reductive dissolution of Fe-(hydroxides. The stabilization processes of As in iron-amended soils under the anoxic and aerobic conditions were characterized by two stages. The increase of crystallization of Fe oxides, decomposition of organic matter, molecular diffusion, and the occlusion within Fe-(hydroxides cocontrolled the transformation of As fractions and the stabilization process of As in iron-amended soils under different redox conditions. In terms of As bioaccessibility, the stabilization process of As in iron-amended soils was shortened under the aerobic condition in comparison with the anoxic condition.

  14. The effect of redox conditions and adaptation time on organic micropollutant removal during river bank filtration: A laboratory-scale column study.

    Science.gov (United States)

    Bertelkamp, C; Verliefde, A R D; Schoutteten, K; Vanhaecke, L; Vanden Bussche, J; Singhal, N; van der Hoek, J P

    2016-02-15

    This study investigated the redox dependent removal and adaptive behaviour of a mixture of 15 organic micropollutants (OMPs) in laboratory-scale soil columns fed with river water. Three separate pilot systems were used consisting of: (1) two columns, (2) ten columns and (3) twenty two columns to create oxic, suboxic (partial nitrate removal) and anoxic (complete nitrate removal). The pilot set-up has some unique features--it can simulate fairly long residence times (e.g., 45 days using the 22 column system) and reduced conditions developed naturally within the system. Dimethoate, diuron, and metoprolol showed redox dependent removal behaviour with higher biodegradation rates in the oxic zone compared to the suboxic/anoxic zone. The redox dependent behaviour of these three OMPs could not be explained based on their physico-chemical properties (hydrophobicity, charge and molecular weight) or functional groups present in the molecular structure. OMPs that showed persistent behaviour in the oxic zone (atrazine, carbamazepine, hydrochlorothiazide and simazine) were also not removed under more reduced conditions. Adaptive behaviour was observed for five OMPs: dimethoate, chloridazon, lincomycin, sulfamethoxazole and phenazone. However, the adaptive behaviour could not be explained by the physico-chemical properties (hydrophobicity, charge and molecular weight) investigated in this study and only rough trends were observed with specific functional groups (e.g. ethers, sulphur, primary and secondary amines). Finally, the adaptive behaviour of OMPs was found to be an important factor that should be incorporated in predictive models for OMP removal during river bank filtration. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Augmenting atrazine and hexachlorobenzene degradation under different soil redox conditions in a bioelectrochemistry system and an analysis of the relevant microorganisms.

    Science.gov (United States)

    Wang, Hui; Cao, Xian; Li, Lei; Fang, Zhou; Li, Xianning

    2018-01-01

    Soil microbial fuel cells (MFCs) are a sustainable technology that degrades organic pollutants while generating electricity. However, there have been no detailed studies of the mechanisms of pollutant degradation in soil MFCs. In this study, the effects of external resistance and electrode effectiveness on atrazine and hexachlorobenzene (HCB) degradation were evaluated, the performance of soil MFCs in the degradation of these pollutants under different soil redox conditions was assessed, and the associated microorganisms in the anode were investigated. With an external resistance of 20Ω, the degradation efficiencies of atrazine and HCB were 95% and 78%, respectively. The degradation efficiency, degradation rate increased with decreasing external resistance, while the half-life decreased. There were different degradation trends for different pollutants under different soil redox conditions. The fastest degradation rate of atrazine was in the upper MFC section (aerobic), whereas that of HCB was in the lower MFC section (anaerobic). The results showed that electrode effectiveness played a significant role in pollution degradation. In addition, the microbial community analysis demonstrated that Proteobacteria, especially Deltaproteobacteria involved in current generation was extremely abundant (27.49%) on soil MFC anodes, although the percentage abundances of atrazine degrading Rhodocyclaceae (8.77%), Desulfitobacterium (0.64%), and HCB degrading Desulfuromonas (0.73%), were considerably lower. The results of the study suggested that soil MFCs can enhance the degradation of atrazine and HCB, and bioelectrochemical reduction was the main mechanism for the pollutants degradation. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. The reactivity of Fe(II) associated with goethite formed during short redox cycles toward Cr(VI) reduction under oxic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Tomaszewski, Elizabeth J.; Lee, Seungyeol; Rudolph, Jared; Xu, Huifang; Ginder-Vogel, Matthew (UW)

    2017-08-01

    Chromium (Cr) is a toxic metal that causes a myriad of health problems and enters the environment as a result of anthropogenic activities and/or natural processes. The toxicity and solubility of chromium is linked to its oxidation state; Cr(III) is poorly soluble and relatively nontoxic, while Cr(VI) is soluble and a known carcinogen. Solid Fe(II) in iron-bearing minerals, such as pyrite, magnetite, and green rusts, reduce the oxidation state of chromium, reducing its toxicity and mobility. However, these minerals are not the only potential sources of solid-associated Fe(II) available for Cr(VI) reduction. For example, ferric (Fe(III)) (hydr)oxides, such as goethite or hematite, can have Fe(II) in the solid without phase transformation; however, the reactivity of Fe(II) within Fe(III) (hydr)oxides with contaminants, has not been previously investigated. Here, we cyclically react goethite with dissolved Fe(II) followed by dissolved O2, leading to the formation of reactive Fe(II) associated with goethite. In separate reactors, the reactivity of this Fe(II) is probed under oxic conditions, by exposure to chromate (CrO42 -) after either one, two, three or four redox cycles. Cr is not present during redox cycling; rather, it is introduced to a subset of the solid after each oxidation half-cycle. Analysis of X-ray absorption near edge structure (XANES) spectra reveals that the extent of Cr(VI) reduction to Cr(III) depends not only on solid Fe(II) content but also surface area and mean size of ordered crystalline domains, determined by BET surface area analysis and X-ray diffraction (XRD), respectively. Shell-by-shell fitting of the extended X-ray absorption fine structure (EXAFS) spectra demonstrates chromium forms both single and double corner sharing complexes on the surface of goethite, in addition to sorbed Cr(III) species. Finally, transmission electron microscope (TEM) imaging and X-ray energy-dispersive spectroscopy (EDS) illustrate that Cr preferentially

  17. Engineering redox balance through cofactor systems.

    Science.gov (United States)

    Chen, Xiulai; Li, Shubo; Liu, Liming

    2014-06-01

    Redox balance plays an important role in the production of enzymes, pharmaceuticals, and chemicals. To meet the demands of industrial production, it is desirable that microbes maintain a maximal carbon flux towards target metabolites with no fluctuations in redox. This requires functional cofactor systems that support dynamic homeostasis between different redox states or functional stability in a given redox state. Redox balance can be achieved by improving the self-balance of a cofactor system, regulating the substrate balance of a cofactor system, and engineering the synthetic balance of a cofactor system. This review summarizes how cofactor systems can be manipulated to improve redox balance in microbes. Copyright © 2014 Elsevier Ltd. All rights reserved.

  18. Effect of redox conditions on the fate of emergin organic micropollutants during artificial recharge of groundwater: batch experiment

    OpenAIRE

    Barbieri, Manuela

    2011-01-01

    La recarrega artificial de les aigües subterrànies consisteix en infiltrar aigua als aqüífers per mitjà de les instal·lacions dissenyades per a tal fi i representa una eina important en la gestió dels recursos. A més de l’augment dels recursos d'aigües subterrànies, la recàrrega pot suposar una millora natural de la seva qualitat durant el seu pas pel subsòl. Els processos que tenen lloc en el sistema sòl-aqüífer (filtració, adsorció, reaccions de mescla , redox, biodegradació, et...

  19. The Association of Arsenic With Redox Conditions, Depth, and Ground-Water Age in the Glacial Aquifer System of the Northern United States

    Science.gov (United States)

    Thomas, Mary Ann

    2007-01-01

    More than 800 wells in the glacial aquifer system of the Northern United States were sampled for arsenic as part of U.S. Geological Survey National Water-Quality Assessment (NAWQA) studies during 1991-2003. Elevated arsenic concentrations (greater than or equal to 10 micrograms per liter) were detected in 9 percent of samples. Elevated arsenic concentrations were associated with strongly reducing conditions. Of the samples classified as iron reducing or sulfate reducing, arsenic concentrations were elevated in 19 percent. Of the methanogenic samples, arsenic concentrations were elevated in 45 percent. In contrast, concentrations of arsenic were elevated in only 1 percent of oxic samples. Arsenic concentrations were also related to ground-water age. Elevated arsenic concentrations were detected in 34 percent of old waters (recharged before 1953) as compared to 4 percent of young waters (recharged since 1953). For samples classified as both old and methanogenic, elevated arsenic concentrations were detected in 62 percent of samples, as compared to 1 percent for samples classified as young and oxic. Arsenic concentrations were also correlated with well depth and concentrations of several chemical constituents, including (1) constituents linked to redox processes and (2) anions or oxyanions that sorb to iron oxides. Observations from the glacial aquifer system are consistent with the idea that the predominant source of arsenic is iron oxides and the predominant mechanism for releasing arsenic to the ground water is reductive desorption or reductive dissolution. Arsenic is also released from iron oxides under oxic conditions, but on a more limited basis and at lower concentrations. Logistic regression was used to investigate the relative significance of redox, ground-water age, depth, and other water-quality constituents as indicators of elevated arsenic concentrations in the glacial aquifer system. The single variable that explained the greatest amount of variation in

  20. The effects of the biogeochemical properties of clay minerals on the Pb sorption and desorption in various redox condition

    Science.gov (United States)

    Koo, T. H.; Kim, J. Y.; Kim, J. W.

    2016-12-01

    The fate and transportation of hazardous trace metal in soil environment can be controlled by various factors including temperature, geological location, properties of bed rock or sediment, human behavior, and biogeochemical reactions. The sorption and desorption process is one of the major process for control the transportation of trace metal in soil-water system. Nonetheless, few studies were focused on the biological controlling parameters, particularly redox reaction of structural metal of clay minerals. Thus, the objective of the present study is to investigate the correlation between the sorption and desorption reaction of Pb and biogeochemical properties of clay minerals. The effects of redox state of structural Fe and layer charge of the minerals on the migration/speciation of Pb at the various geochemical environment will be elucidated. The Fe-rich smectite, nontronite (NAu-1), and bulk soil samples which were collected from abandoned mine areas were reduced by microbial respiration by Shewanella Oneidensis MR-1 and/or Na-dithionite to various oxidation state of structural Fe. Then the Pb-stock solution made with common lead and nitric acid were spiked into the mineral/soil slurry with various Pb concentration to test the sorption and desorption reaction upto 7 days. The reaction was stopped at each time point by freezing the pellet and supernatant separately after centrifugation. Then the concentration and stable isotope ratio of Pb in the supernatant were measured using Inductively Coupled Plasma Mass Spectrometer (ICP-MS) and Multicollector (MC)-ICP-MS. The structural as well as chemical modification on nontronite and bulk soil sample were measured using x-ray diffraction (XRD), scanning electron microscopy (SEM) and wet chemistry analysis. The changes in Pb species in supernatant by sorption and desorption and its consequences on the clay structural/biogeochemical properties will be discussed.

  1. Attenuation of bulk organic matter, nutrients (N and P), and pathogen indicators during soil passage: Effect of temperature and redox conditions in simulated soil aquifer treatment (SAT)

    KAUST Repository

    Abel, Chol D T

    2012-07-22

    Soil aquifer treatment (SAT) is a costeffective natural wastewater treatment and reuse technology. It is an environmentally friendly technology that does not require chemical usage and is applicable to both developing and developed countries. However, the presence of organic matter, nutrients, and pathogens poses a major health threat to the population exposed to partially treated wastewater or reclaimed water through SAT. Laboratory-based soil column and batch experiments simulating SAT were conducted to examine the influence of temperature variation and oxidation-reduction (redox) conditions on removal of bulk organic matter, nutrients, and indicator microorganisms using primary effluent. While an average dissolved organic carbon (DOC) removal of 17.7 % was achieved in soil columns at 5 °C, removal at higher temperatures increased by 10 % increments with increase in temperature by 5 °C over the range of 15 to 25 °C. Furthermore, soil column and batch experiments conducted under different redox conditions revealed higher DOC removal in aerobic (oxic) experiments compared to anoxic experiments. Aerobic soil columns exhibited DOC removal 15 % higher than that achieved in the anoxic columns, while aerobic batch showed DOC removal 7.8 % higher than the corresponding anoxic batch experiments. Ammonium-nitrogen removal greater than 99 % was observed at 20 and 25 °C, while 89.7 % was removed at 15 °C, but the removal substantially decreased to 8.8 % at 5 °C. While ammonium-nitrogen was attenuated by 99.9 % in aerobic batch reactors carried out at room temperature, anoxic experiments under similar conditions revealed 12.1 % ammonium-nitrogen reduction, corresponding to increase in nitrate-nitrogen and decrease in sulfate concentration. © Springer Science+Business Media B.V. 2012.

  2. Combination of aquifer thermal energy storage and enhanced bioremediation: resilience of reductive dechlorination to redox changes.

    Science.gov (United States)

    Ni, Zhuobiao; van Gaans, Pauline; Smit, Martijn; Rijnaarts, Huub; Grotenhuis, Tim

    2016-04-01

    To meet the demand for sustainable energy, aquifer thermal energy storage (ATES) is widely used in the subsurface in urban areas. However, contamination of groundwater, especially with chlorinated volatile organic compounds (CVOCs), is often being encountered. This is commonly seen as an impediment to ATES implementation, although more recently, combining ATES and enhanced bioremediation of CVOCs has been proposed. Issues to be addressed are the high water flow velocities and potential periodic redox fluctuation that accompany ATES. A column study was performed, at a high water flow velocity of 2 m/h, simulating possible changes in subsurface redox conditions due to ATES operation by serial additions of lactate and nitrate. The impacts of redox changes on reductive dechlorination as well as the microbial response of Dehalococcoides (DHC) were evaluated. The results showed that, upon lactate addition, reductive dechlorination proceeded well and complete dechlorination from cis-DCE to ethene was achieved. Upon subsequent nitrate addition, reductive dechlorination immediately ceased. Disruption of microorganisms' retention was also immediate and possibly detached DHC which preferred attaching to the soil matrix under biostimulation conditions. Initially, recovery of dechlorination was possible but required bioaugmentation and nutrient amendment in addition to lactate dosing. Repeated interruption of dechlorination and DHC activity by nitrate dosing appeared to be less easily reversible requiring more efforts for regenerating dechlorination. Overall, our results indicate that the microbial resilience of DHC in biosimulated ATES conditions is sensitive to redox fluctuations. Hence, combining ATES with bioremediation requires dedicated operation and monitoring on the aquifer geochemical conditions.

  3. Biogeochemical reactive-transport modelling of the interactions of medium activity long-lived nuclear waste in fractured argillite and the effect on redox conditions

    International Nuclear Information System (INIS)

    Small, J.S.; Steele, H.; Kwong, S.; Albrecht, A.

    2010-01-01

    Document available in extended abstract form only. The role of anaerobic microbial processes in mediating gas generation and redox reactions in organic (cellulose) containing low level activity nuclear wastes (LLW) is well established through monitoring of operational near-surface LLW disposal sites and municipal waste disposal sites. Modelling approaches based on Monod kinetic growth models to represent the complex suite of anaerobic processes have been developed and these models are able to reproduce the evolving biogeochemistry and gas generation of large scale and long term (10 year) experiments on cellulose waste degradation. In the case of geological disposal of medium activity long-lived nuclear waste (MAVL) microbial processes have the potential to exploit metabolic energy sources present in the waste, engineered barriers and host geological formation and as a consequence influence redox potential. Several electron donors and electron acceptors may be present in MAVL. Electron donors include; hydrogen (resulting from radiolysis and anaerobic corrosion of metals), and hydrolysis products of organic waste materials. Sulphate, nitrate and Fe(III) containing minerals and corrosion products are examples of electron acceptors present in intermediate level wastes. Significant amounts of organic matter, sulphate and iron minerals may also be present in host geological formations and have the potential to act as microbial energy sources once the system is perturbed by electron donors/acceptors from the waste. The construction of a geological disposal facility will physically disturb the host formation, potentially causing fracturing of the excavation damage zone (EDZ). The EDZ may thus provide environmental conditions, such as space and free water that together with nutrient and energy sources to promote microbial activity. In this study the Generalised Repository Model (GRM) developed to simulate the coupled microbiological, chemical and transport processes in near

  4. Effects of Varying RedoxConditions on Natural Attenuation of Inorganic Contaminants from the D-Area Coal Pile Runoff Basin (U)

    Energy Technology Data Exchange (ETDEWEB)

    Kaplan, D

    2004-05-30

    The objective of this study was to provide geochemical parameters to characterize the D-Area Coal Pile Runoff Basin (DCPRB) sediment as a potential source term. It is anticipated that the measured values will be used in risk calculations and will provide additional technical support for imposing Monitored Natural Attenuation at D-Area. This study provides a detailed evaluation of the DCPRB sediment and is part of another study that quantified the Monitored Natural Attenuation of inorganic contaminants more broadly at the D-Area Expanded Operable Unit, which includes the DCPRB (Powell et al. 2004). Distribution coefficients (K{sub d} values; a solid to liquid contaminant concentration ratio) and the Potentially Leachable Fraction (the percent of the total contaminant concentration in the sediment that can likely contribute to a contaminant plume) were measured in a DCPRB sediment as a function of redox conditions. Redox conditions at the DCPRB are expected to vary greatly as the system undergoes varying drying and flooding conditions. Conservative values; K{sub d} values that err on the side of being too low and Potentially Leachable Fraction values that err on the side of being too high, are presented. The K{sub d} values are high compared to conservative literature values, and underscores the importance of measuring site-specific values to provide estimates of sediments natural attenuation/sorption capacities. The Potentially Leachable Fraction indicates that as little as 27% of the As, but all of the Cu and Tl will be part of the source term. In the case of the As, the remaining 83% will likely never leach out of the sediment, thereby providing a form of natural attenuation. Importantly, Be, Cr, Cu, Ni, and V concentrations in the sediment were less-than twice background levels, indicating this sediment was not a potential source for these contaminants. K{sub d} values generally increased significantly (As, Cd, Co, Cr, Cu, Ni, Se, and Tl) when the sediment was

  5. Laminated sediments from the central Peruvian continental slope: A 500 year record of upwelling system productivity, terrestrial runoff and redox conditions

    Science.gov (United States)

    Sifeddine, A.; Gutiérrez, D.; Ortlieb, L.; Boucher, H.; Velazco, F.; Field, D.; Vargas, G.; Boussafir, M.; Salvatteci, R.; Ferreira, V.; García, M.; Valdés, J.; Caquineau, S.; Mandeng Yogo, M.; Cetin, F.; Solis, J.; Soler, P.; Baumgartner, T.

    2008-10-01

    Sedimentological studies including X-ray digital analyses, mineralogy, inorganic contents, and organic geochemistry on cores of laminated sediments accumulated in the oxygen minimum zone of the central Peruvian margin reveal variable oceanographic and climate conditions during the last 500 yr. Coherent upcore variations in sedimentological and geochemical markers in box cores taken off Pisco (B0405-6) and Callao (B0405-13) indicate that variability in the climate proxies examined has regional significance. Most noteworthy is a large shift in proxies at ˜1820 AD, as determined by 210Pb and 14C radiometric dating. This shift is characterized by an increase in total organic carbon (TOC) in parallel with an abrupt increase in the enrichment factor for molybdenum Mo indicating a regional intensification of redox conditions, at least at the sediment water interface. In addition there was lower terrestrial input of quartz, feldspar and clays to the margin. Based on these results, we interpret that during several centuries prior to 1820, which corresponds to the little ice age (LIA), the northern Humboldt current region was less productive and experienced higher terrestrial input related to more humid conditions on the continent. These conditions were probably caused by a southward displacement of the inter-tropical convergence zone and the subtropical high pressure cell during the LIA. Since 1870, increases in TOC and terrigenous mineral fluxes suggest an increase of wind-driven upwelling and higher productivity. These conditions continued to intensify during the late 20th century, as shown by instrumental records of wind forcing.

  6. Controls on the evolution of Ediacaran metazoan ecosystems: A redox perspective.

    Science.gov (United States)

    Bowyer, F; Wood, R A; Poulton, S W

    2017-07-01

    A growing number of detailed geochemical studies of Ediacaran (635-541 Ma) marine successions have provided snapshots into the redox environments that played host to the earliest known metazoans. Whilst previous compilations have focused on the global evolution of Ediacaran water column redox chemistry, the inherent heterogeneity evident in palaeogeographically distinct environments demands a more dissected approach to better understand the nature, interactions and evolution of extrinsic controls on the development of early macrobenthic ecosystems. Here, we review available data of local-scale redox conditions within a palaeogeographic and sequence stratigraphic framework, to explore the mechanisms controlling water column redox conditions and their potential impact on the record of metazoans. The openly connected Laurentian margin, North America (632-540 Ma) and Nama basin, Namibia (550-538 Ma), and the variably restricted Yangtze Block, South China (635-520 Ma), show continued redox instability after the first fossil evidence for metazoans. This may support opportunistic benthic colonisation during periods of transient oxygenation amidst episodic upwelling of anoxic waters beneath a very shallow, fluctuating chemocline. The first skeletal metazoans appeared under conditions of continued redox stratification, such as those which characterise the Dengying Formation of the Yangtze Block and the Kuibis Subgroup of the Nama basin. Current data, however, suggests that successful metazoan reef-building demanded more persistent oxia. We propose that cratonic positioning and migration throughout the Ediacaran Period, in combination with gradually increasing dissolved oxygen loading, may have provided a first-order control on redox evolution through regulating circulation mechanisms in the Mirovian Ocean. Some unrestricted lower slope environments from mid-high latitudes benefited from sustained oxygenation via downwelling, whilst transit of isolated cratons towards

  7. Anaerobic degradation of a mixture of MtBE, EtBE, TBA, and benzene under different redox conditions.

    Science.gov (United States)

    van der Waals, Marcelle J; Pijls, Charles; Sinke, Anja J C; Langenhoff, Alette A M; Smidt, Hauke; Gerritse, Jan

    2018-04-01

    The increasing use of biobased fuels and fuel additives can potentially change the typical fuel-related contamination in soil and groundwater. Anaerobic biotransformation of the biofuel additive ethyl tert-butyl ether (EtBE), as well as of methyl tert-butyl ether (MtBE), benzene, and tert-butyl alcohol (TBA, a possible oxygenate metabolite), was studied at an industrially contaminated site and in the laboratory. Analysis of groundwater samples indicated that in the field MtBE was degraded, yielding TBA as major product. In batch microcosms, MtBE was degraded under different conditions: unamended control, with medium without added electron acceptors, or with ferrihydrite or sulfate (with or without medium) as electron acceptor, respectively. Degradation of EtBE was not observed under any of these conditions tested. TBA was partially depleted in parallel with MtBE. Results of microcosm experiments with MtBE substrate analogues, i.e., syringate, vanillate, or ferulate, were in line with the hypothesis that the observed TBA degradation is a cometabolic process. Microcosms with ferulate, syringate, isopropanol, or diethyl ether showed EtBE depletion up to 86.5% of the initial concentration after 83 days. Benzene was degraded in the unamended controls, with medium without added electron acceptors and with ferrihydrite, sulfate, or chlorate as electron acceptor, respectively. In the presence of nitrate, benzene was only degraded after addition of an anaerobic benzene-degrading community. Nitrate and chlorate hindered MtBE, EtBE, and TBA degradation.

  8. Pressure Fluctuations on the Bed of Surge Tank at the H.P. Zimapan, Hgo., with Different Arrangements Studied on Hydraulic Model, with the Lowest Operation Conditions

    Directory of Open Access Journals (Sweden)

    H. Marengo–Mogollón

    2009-10-01

    Full Text Available In this paper, the pressure fluctuations of the surge tank in the Zimapan Hydroelectric Project are compared in a hydraulic model. The shaft is located lateral, over the conduction tunnel and in the simple form (permitting the tunnel entering the shaft, with and without orifice plates taking into account the demand and supply condition of energy with the minimum level of water of the conduction. It was determined the hydraulic efficiency and it was found that it was the best constructive option.

  9. Assessment of the performance of SMFCs in the bioremediation of PAHs in contaminated marine sediments under different redox conditions and analysis of the associated microbial communities

    KAUST Repository

    Hamdan, Hamdan Z.

    2016-10-09

    The biodegradation of naphthalene, 2-methylnaphthalene and phenanthrene was evaluated in marine sediment microbial fuel cells (SMFCs) under different biodegradation conditions, including sulfate reduction as a major biodegradation pathway, employment of anode as terminal electron acceptor (TEA) under inhibited sulfate reducing bacteria activity, and combined sulfate and anode usage as electron acceptors. A significant removal of naphthalene and 2-methylnaphthalene was observed at early stages of incubation in all treatments and was attributed to their high volatility. In the case of phenanthrene, a significant removal (93.83 ± 1.68%) was measured in the closed circuit SMFCs with the anode acting as the main TEA and under combined anode and sulfate reduction conditions (88.51 ± 1.3%). A much lower removal (40.37 ± 3.24%) was achieved in the open circuit SMFCs operating with sulfate reduction as a major biodegradation pathway. Analysis of the anodic bacterial community using 16S rRNA gene pyrosequencing revealed the enrichment of genera with potential exoelectrogenic capability, namely Geoalkalibacter and Desulfuromonas, on the anode of the closed circuit SMFCs under inhibited SRB activity, while they were not detected on the anode of open circuit SMFCs. These results demonstrate the role of the anode in enhancing PAHs biodegradation in contaminated marine sediments and suggest a higher system efficiency in the absence of competition between microbial redox processes (under SRB inhibition), namely due to the anode enrichment with exoelectrogenic bacteria, which is a more energetically favorable mechanism for PAHs oxidation than sulfate.

  10. Modelling Salmonella concentration throughout the pork supply chain by considering growth and survival in fluctuating conditions of temperature, pH and a(w).

    Science.gov (United States)

    Pin, Carmen; Avendaño-Perez, Gaspar; Cosciani-Cunico, Elena; Gómez, Natalia; Gounadakic, Antonia; Nychas, George-John; Skandamis, Panos; Barker, Gary

    2011-03-01

    We aim to predict the population density of Salmonella spp. through the pork supply chain under dynamic environmental conditions (pH, a(w) and temperature) that fluctuate from growth to survival/slow inactivation. To do this, the dependence of the probability of growth, and of the growth and inactivation rate on the temperature, pH and a(w) were modelled. Probabilistic and kinetic measurements, i.e. growth and survival curves, were collected from the ComBase database (www.combase.cc). Conditions at which selected data used to fit the models were generated covered wide ranges that are relevant to the pork supply chain. Probabilistic and kinetic models were combined to give predictions on the concentration of Salmonella spp. at any stage of the pork supply chain under fluctuating pH, a(w) and/or temperature. Models were implemented in a user-friendly computing tool freely available from http://www.ifr.ac.uk/safety/SalmonellaPredictions/. This program provides estimates on the population dynamics of Salmonella spp. at any stage of the pork supply chain and its predictive performance has been validated in several pork products. Copyright © 2010 Elsevier B.V. All rights reserved.

  11. Leaf growth rate per unit thermal time follows QTL-dependent daily patterns in hundreds of maize lines under naturally fluctuating conditions.

    Science.gov (United States)

    Sadok, Walid; Naudin, Philippe; Boussuge, Benoit; Muller, Bertrand; Welcker, Claude; Tardieu, Francois

    2007-02-01

    We have analysed daily patterns of leaf elongation rate (LER) in large data sets with 318 genotypes placed in naturally fluctuating temperature and evaporative demand, and examined the effect of targeted alleles on these patterns. The method consisted, firstly, in expressing elongation rate per unit thermal time, so it became temperature independent; secondly, in a joint analysis of diurnal fluctuations of elongation rate and of micrometeorological conditions in several experiments, and finally, in a comparison of daily patterns between groups of genotypes possessing targeted alleles. (1) Conditions for using thermal time at a time step of 15 min were first tested successfully in 318 recombinant inbred lines (RILs) of three mapping populations. (2) An analysis of 1989 time courses revealed a robust daily pattern of LER per unit thermal time (LERth) over several experiments. LERth was constant during the night and was reproducible (for a given RIL) over up to 10 consecutive nights in different experiments. It declined rapidly during the early morning, closely following the daily pattern of transpiration rate. (3) Groups of RILs carrying alleles conferring a high response to temperature had markedly higher night-time plateau of LER than those with low responses. Groups of RILs with high response to evaporative demand had rapid decreases in elongation rate at the transition between night and day, while this decrease was slower in groups of RILs with low response. These results open the way for using kinetics of responses to environmental stimuli as a phenotyping tool in genetic analyses.

  12. Soil Metabolome and Metabolic Fate: Microbial Insights into Freshwater Tidal Wetland Redox Biogeochemistry

    Science.gov (United States)

    Roy Chowdhury, T.; Bramer, L.; Hoyt, D. W.; Kim, Y. M.; Metz, T. O.; McCue, L. A.; Jansson, J.; Bailey, V. L.

    2017-12-01

    Earth System Models predict climate extremes that will impact regional and global hydrology. Aquatic-terrestrial transition zones like wetlands will experience the immediate consequence of climate change as shifts in the magnitude and dynamics of hydrologic flow. Such fluctuating hydrology can alter the structure and function of the soil microbial populations that in turn will alter the nature and rate of biogeochemical transformations and significantly impact the carbon balance of the ecosystem. We tested the impacts of shifting hydrology on the soil microbiome and the role of antecedent moisture condition on redox active microbial processes in soils sampled from a tidal freshwater wetland system in the lower Columbia River, WA, USA. Our objectives were to characterize changes in the soil microbial community composition in response to soil moisture legacy effects, and to elucidate relationships between community response, geochemical signatures and metabolite profiles in this soil. The 16S rRNA gene sequencing showed significant decreases in bacterial abundance capable of anaerobic metabolism in response to drying, but quickly recovered to the antecedent moisture condition, as observed by redox processes. Metabolomics and biogeochemical process rates generated evidence for moisture-driven redox conditions as principal controls on the community and metabolic function. Fluctuating redox conditions altered terminal electron acceptor and donor availability and recovery strengths of these pools in soil such that a disproportionate release of carbon dioxide stemmed from alternative anaerobic degradation processes like sulfate and iron reduction in compared to methanogenesis. Our results show that anoxic conditions impact microbial communities in both permanently and temporarily saturated conditions and that rapid change in hydrology can increase substrate availability for both aerobic and anaerobic decomposition processes, including methanogenesis.

  13. Fully Quantum Fluctuation Theorems

    Science.gov (United States)

    Åberg, Johan

    2018-02-01

    Systems that are driven out of thermal equilibrium typically dissipate random quantities of energy on microscopic scales. Crooks fluctuation theorem relates the distribution of these random work costs to the corresponding distribution for the reverse process. By an analysis that explicitly incorporates the energy reservoir that donates the energy and the control system that implements the dynamic, we obtain a quantum generalization of Crooks theorem that not only includes the energy changes in the reservoir but also the full description of its evolution, including coherences. Moreover, this approach opens up the possibility for generalizations of the concept of fluctuation relations. Here, we introduce "conditional" fluctuation relations that are applicable to nonequilibrium systems, as well as approximate fluctuation relations that allow for the analysis of autonomous evolution generated by global time-independent Hamiltonians. We furthermore extend these notions to Markovian master equations, implicitly modeling the influence of the heat bath.

  14. Redox regulation of mitochondrial proteins and proteomes by cysteine thiol switches.

    Science.gov (United States)

    Nietzel, Thomas; Mostertz, Jörg; Hochgräfe, Falko; Schwarzländer, Markus

    2017-03-01

    Mitochondria are hotspots of cellular redox biochemistry. Respiration as a defining mitochondrial function is made up of a series of electron transfers that are ultimately coupled to maintaining the proton motive force, ATP production and cellular energy supply. The individual reaction steps involved require tight control and flexible regulation to maintain energy and redox balance in the cell under fluctuating demands. Redox regulation by thiol switching has been a long-standing candidate mechanism to support rapid adjustment of mitochondrial protein function at the posttranslational level. Here we review recent advances in our understanding of cysteine thiol switches in the mitochondrial proteome with a focus on their operation in vivo. We assess the conceptual basis for thiol switching in mitochondria and discuss to what extent insights gained from in vitro studies may be valid in vivo, considering thermodynamic, kinetic and structural constraints. We compare functional proteomic approaches that have been used to assess mitochondrial protein thiol switches, including thioredoxin trapping, redox difference gel electrophoresis (redoxDIGE), isotope-coded affinity tag (OxICAT) and iodoacetyl tandem mass tag (iodoTMT) labelling strategies. We discuss conditions that may favour active thiol switching in mitochondrial proteomes in vivo, and appraise recent advances in dissecting their impact using combinations of in vivo redox sensing and quantitative redox proteomics. Finally we focus on four central facets of mitochondrial biology, aging, carbon metabolism, energy coupling and electron transport, exemplifying the current emergence of a mechanistic understanding of mitochondrial regulation by thiol switching in living plants and animals. Copyright © 2016 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

  15. Iron and sulfur isotope constraints on redox conditions associated with the 3.2 Ga barite deposits of the Mapepe Formation (Barberton Greenstone Belt, South Africa)

    Science.gov (United States)

    Busigny, Vincent; Marin-Carbonne, Johanna; Muller, Elodie; Cartigny, Pierre; Rollion-Bard, Claire; Assayag, Nelly; Philippot, Pascal

    2017-08-01

    The occurrence of Early Archean barite deposits is intriguing since this type of sediment requires high availability of dissolved sulfate (SO42-), the oxidized form of sulfur, although most authors argued that the Archean eon was dominated by reducing conditions, with low oceanic sulfate concentration (state of the paleo-atmosphere and -oceans, we examined Fe and S isotope compositions in a sedimentary sequence from the 3.2 Ga-old Mendon and Mapepe formations (Kaapvaal craton, South Africa), recovered from the drill-core BBDP2 of the Barberton Barite Drilling Project. Major elements were also analyzed to constrain the respective imprints of detrital vs metasomatic processes, in particular using Al, Ti and K interrelations. Bulk rock Fe isotope compositions are linked to mineralogy, with δ56Fe values varying between -2.04‰ in Fe sulfide-dominated barite beds, to +2.14‰ in Fe oxide-bearing cherts. δ34S values of sulfides vary between -10.84 and +3.56‰, with Δ33S in a range comprised between -0.35 and +2.55‰, thus supporting an O2-depleted atmosphere (<10-5 PAL). Iron isotope variations together with major element correlations show that, although the sediments experienced a pervasive stage of hydrothermal alteration, the rocks preserved a primary/authigenic signature predating subsequent hydrothermal stage. Highly positive δ56Fe values recorded in primary Fe-oxides from ferruginous cherts support partial Fe oxidation in a reducing oceanic environment (O2 < 10-4 μM), but are incompatible with a model of complete oxidation at the redox boundary of a stratified water column. Iron oxide precipitation under low O2 levels was likely mediated by anoxygenic photosynthesis, and/or abiotic photo-oxidation processes. Our results are consistent with global anoxic conditions in the 3.2 Ga-old sediments, implying that the barite deposits were most likely sourced by atmospheric photolysis of S gases produced by large subaerial volcanic events, and possibly SO42

  16. Quantum fluctuations

    International Nuclear Information System (INIS)

    Reynaud, S.; Giacobino, S.; Zinn-Justin, J.

    1997-01-01

    This course is dedicated to present in a pedagogical manner the recent developments in peculiar fields concerned by quantum fluctuations: quantum noise in optics, light propagation through dielectric media, sub-Poissonian light generated by lasers and masers, quantum non-demolition measurements, quantum electrodynamics applied to cavities and electrical circuits involving superconducting tunnel junctions. (A.C.)

  17. Redox regulation of cell proliferation: Bioinformatics and redox proteomics approaches to identify redox-sensitive cell cycle regulators.

    Science.gov (United States)

    Foyer, Christine H; Wilson, Michael H; Wright, Megan H

    2018-03-29

    Plant stem cells are the foundation of plant growth and development. The balance of quiescence and division is highly regulated, while ensuring that proliferating cells are protected from the adverse effects of environment fluctuations that may damage the genome. Redox regulation is important in both the activation of proliferation and arrest of the cell cycle upon perception of environmental stress. Within this context, reactive oxygen species serve as 'pro-life' signals with positive roles in the regulation of the cell cycle and survival. However, very little is known about the metabolic mechanisms and redox-sensitive proteins that influence cell cycle progression. We have identified cysteine residues on known cell cycle regulators in Arabidopsis that are potentially accessible, and could play a role in redox regulation, based on secondary structure and solvent accessibility likelihoods for each protein. We propose that redox regulation may function alongside other known posttranslational modifications to control the functions of core cell cycle regulators such as the retinoblastoma protein. Since our current understanding of how redox regulation is involved in cell cycle control is hindered by a lack of knowledge regarding both which residues are important and how modification of those residues alters protein function, we discuss how critical redox modifications can be mapped at the molecular level. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

  18. Zinc and the modulation of redox homeostasis

    Science.gov (United States)

    Oteiza, Patricia I.

    2012-01-01

    Zinc, a redox inactive metal, has been long viewed as a component of the antioxidant network, and growing evidence points to its involvement in redox-regulated signaling. These actions are exerted through several mechanisms based on the unique chemical and functional properties of zinc. Overall, zinc contributes to maintain the cell redox balance through different mechanisms including: i) the regulation of oxidant production and metal-induced oxidative damage; ii) the dynamic association of zinc with sulfur in protein cysteine clusters, from which the metal can be released by nitric oxide, peroxides, oxidized glutathione and other thiol oxidant species; iii) zinc-mediated induction of the zinc-binding protein metallothionein, which releases the metal under oxidative conditions and act per se scavenging oxidants; iv) the involvement of zinc in the regulation of glutathione metabolism and of the overall protein thiol redox status; and v) a direct or indirect regulation of redox signaling. Findings of oxidative stress, altered redox signaling, and associated cell/tissue disfunction in cell and animal models of zinc deficiency, stress the relevant role of zinc in the preservation of cell redox homeostasis. However, while the participation of zinc in antioxidant protection, redox sensing, and redox-regulated signaling is accepted, the involved molecules, targets and mechanisms are still partially known and the subject of active research. PMID:22960578

  19. Redox regulation of plant development.

    Science.gov (United States)

    Considine, Michael J; Foyer, Christine H

    2014-09-20

    We provide a conceptual framework for the interactions between the cellular redox signaling hub and the phytohormone signaling network that controls plant growth and development to maximize plant productivity under stress-free situations, while limiting growth and altering development on exposure to stress. Enhanced cellular oxidation plays a key role in the regulation of plant growth and stress responses. Oxidative signals or cycles of oxidation and reduction are crucial for the alleviation of dormancy and quiescence, activating the cell cycle and triggering genetic and epigenetic control that underpin growth and differentiation responses to changing environmental conditions. The redox signaling hub interfaces directly with the phytohormone network in the synergistic control of growth and its modulation in response to environmental stress, but a few components have been identified. Accumulating evidence points to a complex interplay of phytohormone and redox controls that operate at multiple levels. For simplicity, we focus here on redox-dependent processes that control root growth and development and bud burst. The multiple roles of reactive oxygen species in the control of plant growth and development have been identified, but increasing emphasis should now be placed on the functions of redox-regulated proteins, along with the central roles of reductants such as NAD(P)H, thioredoxins, glutathione, glutaredoxins, peroxiredoxins, ascorbate, and reduced ferredoxin in the regulation of the genetic and epigenetic factors that modulate the growth and vigor of crop plants, particularly within an agricultural context.

  20. State conditions transferability of vapor-liquid equilibria via fluctuation solution theory with correlation function integrals from molecular dynamics simulations

    DEFF Research Database (Denmark)

    Christensen, Steen; Peters, Günther H.J.; Hansen, Flemming Yssing

    2007-01-01

    –457] comprise the nearly ideal benzene/methyl acetate system, and the less ideal benzene/ethanol system at ambient temperatures. Both are at low pressures and remote from the pure component critical points. For the IFPSC system, we have used the same method even though predictions are for conditions remote from...... on isobaric–isothermal molecular dynamics (NPT-MD) simulations, using force field parameters published in the literature and fitted CHARMM force field parameters. Systems studied previously [S. Christensen, G.H. Peters, F.Y. Hansen, J.P. O’Connell, J. Abildskov, Molecular Simulation 33 (2007) 449...... those of the provided data, the pressures are elevated, and the temperatures are near the critical temperature of one of the components. We first describe the computational method and thermodynamic modeling for the entry submitted, which assumed the vapor was an ideal gas and no Poynting correction...

  1. Bioconversion of Coal: Hydrologic indicators of the extent of coal biodegradation under different redox conditions and coal maturity, Velenje Basin case study, Slovenia

    Science.gov (United States)

    Kanduč, Tjaša; Grassa, Fausto; Lazar, Jerneja; Jamnikar, Sergej; Zavšek, Simon; McIntosh, Jennifer

    2014-05-01

    Underground mining of coal and coal combustion for energy has significant environmental impacts. In order to reduce greenhouse gas emissions, other lower -carbon energy sources must be utilized. Coalbed methane (CBM) is an important source of relatively low-carbon energy. Approximately 20% of world's coalbed methane is microbial in origin (Bates et al., 2011). Interest in microbial CBM has increased recently due to the possibility of stimulating methanogenesis. Despite increasing interest, the hydrogeochemical conditions and mechanisms for biodegradation of coal and microbial methane production are poorly understood. This project aims to examine geochemical characteristics of coalbed groundwater and coalbed gases in order to constrain biogeochemical processes to better understand the entire process of coal biodegradation of coal to coalbed gases. A better understanding of geochemical processes in CBM areas may potentially lead to sustainable stimulation of microbial methanogenesis at economical rates. Natural analogue studies of carbon dioxide occurring in the subsurface have the potential to yield insights into mechanisms of carbon dioxide storage over geological time scales (Li et al., 2013). In order to explore redox processes related to methanogenesis and determine ideal conditions under which microbial degradation of coal is likely to occur, this study utilizes groundwater and coalbed gas samples from Velenje Basin. Determination of the concentrations of methane, carbondioxide, nitrogen, oxygen, argon was performed with homemade NIER mass spectrometer. Isotopic composition of carbon dioxide, isotopic composition of methane, isotopic composition of deuterium in methane was determined with Europa-Scientific IRMS with an ANCA-TG preparation module and Thermo Delta XP GC-TC/CF-IRMS coupled to a TRACE GC analyzer. Total alkalinity of groundwater was measured by Gran titration. Major cations were analyzed by ICP-OES and anions by IC method. Isotopic composition of

  2. Redox Behavior of Fe2+/Fe3+ Redox Couple by Absorption Spectroscopy and Measurement

    International Nuclear Information System (INIS)

    Oh, J. Y.; Park, S.; Yun, J. I.

    2010-01-01

    Redox behavior has influences on speciation and other geochemical reactions of radionuclides such as sorption, solubility, and colloid formation, etc. It is one of the factors for evaluation of long-term safety assessment under high-level radioactive waste (HLW) disposal conditions. Accordingly, redox potential (Eh) measurement in aquatic system is important to investigate the redox conditions. Eh is usually measured with redox active electrodes (Pt, Au, glassy carbon, etc.). Nevertheless, Eh measurements by general methods using electrodes provide low accuracy and high uncertainty problem. Therefore, Eh calculated from the concentration of redox active elements with a proper complexing reagent by using UV-Vis absorption spectroscopy is progressed. Iron exists mostly as spent nuclear waste container material and in hydro-geologic minerals. In this system, iron controls the redox condition in near-field area and influences chemical behavior and speciation of radionuclides including redox sensitive actinides such as U, Np, and Pu. In the present work, we present the investigation on redox phenomena of iron in aquatic system by a combination of absorption spectroscopy and redox potential measurements

  3. Plant redox proteomics

    DEFF Research Database (Denmark)

    Navrot, Nicolas; Finnie, Christine; Svensson, Birte

    2011-01-01

    In common with other aerobic organisms, plants are exposed to reactive oxygen species resulting in formation of post-translational modifications related to protein oxidoreduction (redox PTMs) that may inflict oxidative protein damage. Accumulating evidence also underscores the importance of redox...... PTMs in regulating enzymatic activities and controlling biological processes in plants. Notably, proteins controlling the cellular redox state, e.g. thioredoxin and glutaredoxin, appear to play dual roles to maintain oxidative stress resistance and regulate signal transduction pathways via redox PTMs....... To get a comprehensive overview of these types of redox-regulated pathways there is therefore an emerging interest to monitor changes in redox PTMs on a proteome scale. Compared to some other PTMs, e.g. protein phosphorylation, redox PTMs have received less attention in plant proteome analysis, possibly...

  4. Redox activity of naphthalene secondary organic aerosol

    Science.gov (United States)

    McWhinney, R. D.; Zhou, S.; Abbatt, J. P. D.

    2013-04-01

    Chamber secondary organic aerosol (SOA) from low-NOx photooxidation of naphthalene by hydroxyl radical was examined with respect to its redox cycling behaviour using the dithiothreitol (DTT) assay. Naphthalene SOA was highly redox active, consuming DTT at an average rate of 118 ± 14 pmol per minute per μg of SOA material. Measured particle-phase masses of the major previously identified redox active products, 1,2- and 1,4-naphthoquinone, accounted for only 21 ± 3% of the observed redox cycling activity. The redox-active 5-hydroxy-1,4-naphthoquinone was identified as a new minor product of naphthalene oxidation, and including this species in redox activity predictions increased the predicted DTT reactivity to 30 ± 5% of observations. Similar attempts to predict redox behaviour of oxidised two-stroke engine exhaust particles by measuring 1,2-naphthoquinone, 1,4-naphthoquinone and 9,10-phenanthrenequinone predicted DTT decay rates only 4.9 ± 2.5% of those observed. Together, these results suggest that there are substantial unidentified redox-active SOA constituents beyond the small quinones that may be important toxic components of these particles. A gas-to-SOA particle partitioning coefficient was calculated to be (7.0 ± 2.5) × 10-4 m3 μg-1 for 1,4-naphthoquinone at 25 °C. This value suggests that under typical warm conditions, 1,4-naphthoquinone is unlikely to contribute strongly to redox behaviour of ambient particles, although further work is needed to determine the potential impact under conditions such as low temperatures where partitioning to the particle is more favourable. As well, higher order oxidation products that likely account for a substantial fraction of the redox cycling capability of the naphthalene SOA are likely to partition much more strongly to the particle phase.

  5. Chloroplast Redox Poise

    DEFF Research Database (Denmark)

    Steccanella, Verdiana

    The redox state of the chloroplast is maintained by a delicate balance between energy production and consumption and is affected by the need to avoid increased production of reactive oxygen species (ROS). Redox power and ROS generated in the chloroplast are essential for maintaining physiological...... the redox status of the plastoquinone pool and chlorophyll biosynthesis. Furthermore, in the plant cell, the equilibrium between redox reactions and ROS signals is also maintained by various balancing mechanisms among which the thioredoxin reductase-thioredoxin system (TR-Trx) stands out as a mediator...

  6. Evaluation of I/Ca ratios in benthic foraminifera from the Peruvian oxygen minimum zone as proxy for redox conditions in the ambient water masses

    Science.gov (United States)

    Glock, N.; Liebetrau, V.; Eisenhauer, A.

    2014-12-01

    Tropical oxygen minimum zones (OMZs) are most important areas of oxygen depletion in today´s oceans and nutrient cycling in these regions has a large socio-economic impact because they account for about 17% of the global commercial fish catches(1). Possibly increasing magnitude and area of oxygen depletion in these regions, might endanger rich pelagic fish habitats in the future threatening the global marine food supply. By the use of a quantitative redox proxy in OMZs, reconstruction of the temporal variation in OMZ extension eventually providing information about past and future changes in oxygenation and the anthropogenic role in the recent trend of expanding OMZs(2). Recent work has shown that iodine/calcium (I/Ca) ratios in marine carbonates are a promising proxy for ambient oxygen concentration(3). Our study explores the correlation of I/Ca ratios in four benthic foraminiferal species (three calcitic, one aragonitic) from the Peruvian OMZ to bottom water oxygen concentrations ([O2]BW) and evaluates foraminiferal I/Ca ratios as a possible redox proxy for the ambient water masses. Our results show that all species have a positive trend in the I/Ca ratios as a function of [O2]BW. Only for the aragonitic species Hoeglundina elegans this trend is not significant. The highest significance has been found for Uvigerina striata (I/Ca = 0.032(±0.004).[O2]BW + 0.29(±0.03), R² = 0.61, F = 75, P solutions, (ii) a species dependency of the I/Ca-[O2]BW relationship which is either related to a strong vital effect or toa species dependency on the calcification depth within sediment, and (iii) the inter-test variability of I/Ca between different specimens from the same species and habitat. (1): FAO FishStat: Fisheries and aquaculture software. In: FAO Fisheries and Aquaculture Department[online]. Rome. Updated 28 Nov. 2013. (2): Stramma et al.: Expanding Oxygen-Minimum Zones in the Tropical Oceans, Science, 320, 655-658, 2008. (3): Lu et al.: Iodine to calcium ratios in

  7. Redox signaling in plants.

    Science.gov (United States)

    Foyer, Christine H; Noctor, Graham

    2013-06-01

    Our aim is to deliver an authoritative and challenging perspective of current concepts in plant redox signaling, focusing particularly on the complex interface between the redox and hormone-signaling pathways that allow precise control of plant growth and defense in response to metabolic triggers and environmental constraints and cues. Plants produce significant amounts of singlet oxygen and other reactive oxygen species (ROS) as a result of photosynthetic electron transport and metabolism. Such pathways contribute to the compartment-specific redox-regulated signaling systems in plant cells that convey information to the nucleus to regulate gene expression. Like the chloroplasts and mitochondria, the apoplast-cell wall compartment makes a significant contribution to the redox signaling network, but unlike these organelles, the apoplast has a low antioxidant-buffering capacity. The respective roles of ROS, low-molecular antioxidants, redox-active proteins, and antioxidant enzymes are considered in relation to the functions of plant hormones such as salicylic acid, jasmonic acid, and auxin, in the composite control of plant growth and defense. Regulation of redox gradients between key compartments in plant cells such as those across the plasma membrane facilitates flexible and multiple faceted opportunities for redox signaling that spans the intracellular and extracellular environments. In conclusion, plants are recognized as masters of the art of redox regulation that use oxidants and antioxidants as flexible integrators of signals from metabolism and the environment.

  8. Controls on the redox potential of rainwater.

    Science.gov (United States)

    Willey, Joan D; Mullaugh, Katherine M; Kieber, Robert J; Avery, G Brooks; Mead, Ralph N

    2012-12-18

    Hydrogen peroxide acting as a reductant affects the redox potential of rainwater collected at the Bermuda Atlantic Time Series Station, the South Island of New Zealand, the contiguous USA, and the primary study site in Wilmington, NC. Analytical measurements of both halves of redox couples for dissolved iron, mercury, and the nitrate-nitrite-ammonium system can predict the rainwater redox potential measured directly by a platinum electrode. Measurements of these redox couples along with the pH in rain yields pe⁻ between 8 and 11; the half reaction for hydrogen peroxide acting as a reductant using typical rainwater conditions of 15 μM H₂O₂ at pH 4.7 gives pe⁻ = 9.12, where pe⁻ = negative log of the activity of hydrated electrons. Of the six rainwater redox systems investigated, only manganese speciation appeared to be controlled by molecular oxygen (pe⁻ = 15.90). Copper redox speciation was consistent with superoxide acting as a reductant (pe⁻ = 2.7). The concentration of H₂O₂ in precipitation has more than doubled over the preceding decade due to a decrease in SO₂ emissions, which suggests the redox chemistry of rainwater is dynamic and changing, potentially altering the speciation of many organic compounds and trace metals in atmospheric waters.

  9. Fluctuation analysis

    International Nuclear Information System (INIS)

    Clarke, J.

    1980-01-01

    This paper briefly reviews sources of noise in Josephson junctions, and the limits they impose on the sensitivity of dc and rf SQUIDS. The results are strictly valid only for a resistively shunted junction (RSJ) with zero capacitance, but should be applicable to point contact junctions and microbridges in so far as these devices can be approximated by the RSJ model. Fluctuations arising from Nyquist noise in the resistive shunt of a single junction are discussed in the limit eI/sub o/R/k/sub B/T << 1 in which a classical treatment is appropriate, and then extend the treatment to the limit eI/sub o/R/k/sub B/T greater than or equal to 1 in which quantum effects become important. The Nyquist limit theory is used to calculate the noise in a dc SQUID, and the results are compared with a number of practical devices. The quantum limit is briefly considered. Results for the predicted sensitivity of rf SQUIDS are presented, and also compared with a number of practical devices. Finally, the importance of l/f noise (f is the frequency) in limiting the low frequency performance of SQUIDS is discussed

  10. Redox-gated electron transport in electrically wired ferrocene molecules

    Energy Technology Data Exchange (ETDEWEB)

    Xiao Xiaoyin [Department of Electrical Engineering and Center for Solid State Electronic Research, Arizona State University, Tempe, AZ 85287 (United States); Brune, Daniel [Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 (United States); He Jin [Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, AZ 85287 (United States); Lindsay, Stuart [Center for Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, AZ 85287 (United States); Gorman, Christopher B. [Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204 (United States); Tao Nongjian [Department of Electrical Engineering and Center for Solid State Electronic Research, Arizona State University, Tempe, AZ 85287 (United States)], E-mail: nongjian.tao@asu.edu

    2006-07-11

    We have synthesized cysteamine-terminated ferrocene molecules and determined the dependence of the electron transport properties of the molecules on their redox states by measuring the current through the molecules as a function of the electrode potential. The current fluctuates over a large range, but its average value increases with the potential. We attribute the current fluctuation and its increase with the potential to the switching of the molecules from low-conductance reduced state to high-conductance oxidized state.

  11. Redox-gated electron transport in electrically wired ferrocene molecules

    International Nuclear Information System (INIS)

    Xiao Xiaoyin; Brune, Daniel; He Jin; Lindsay, Stuart; Gorman, Christopher B.; Tao Nongjian

    2006-01-01

    We have synthesized cysteamine-terminated ferrocene molecules and determined the dependence of the electron transport properties of the molecules on their redox states by measuring the current through the molecules as a function of the electrode potential. The current fluctuates over a large range, but its average value increases with the potential. We attribute the current fluctuation and its increase with the potential to the switching of the molecules from low-conductance reduced state to high-conductance oxidized state

  12. Causal signal transmission by quantum fields. VI: The Lorentz condition and Maxwell’s equations for fluctuations of the electromagnetic field

    International Nuclear Information System (INIS)

    Plimak, L.I.; Stenholm, S.

    2013-01-01

    The general structure of electromagnetic interactions in the so-called response representation of quantum electrodynamics (QED) is analysed. A formal solution to the general quantum problem of the electromagnetic field interacting with matter is found. Independently, a formal solution to the corresponding problem in classical stochastic electrodynamics (CSED) is constructed. CSED and QED differ only in the replacement of stochastic averages of c-number fields and currents by time-normal averages of the corresponding Heisenberg operators. All relations of QED connecting quantum field to quantum current lack Planck’s constant, and thus coincide with their counterparts in CSED. In Feynman’s terms, one encounters complete disentanglement of the potential and current operators in response picture. Based on this parallelism between QED and CSED, it is natural to expect validity of the Lorentz condition and Maxwell’s equations for the time-normal averages of the potential and current. Things however turn out to be more complicated. Maxwell’s equations under the time-normal ordering can only be demonstrated subject to cancellation of the so-called Schwinger terms by gauge-invariant regularisations. We presume this pattern to be general, formulating this as “commutativity conjecture”. Consistency of the latter with the Heisenberg uncertainty principle is discussed. -- Highlights: •The general structure of interaction in quantum electrodynamics (QED) is analysed. •A detailed parallelism between QED and classical stochastic electrodynamics is shown. •Validity of Maxwell’s equations for fluctuations of the field is discussed. •This validity turns out to be in essence a renormalisation postulate

  13. Iron Redox Dynamics in Humid Tropical Forest Soils: Carbon Stabilization vs. Degradation?

    Science.gov (United States)

    Hall, S. J.; Silver, W. L.; Hammel, K.

    2015-12-01

    Most terrestrial soils exhibit a patchwork of oxygen (O2) availability that varies over spatial scales of microsites to catenas to landscapes, and over temporal scales of minutes to seasons. Oxygen fluctuations often drive microbial iron (Fe) reduction and abiotic/biotic Fe oxidation at the microsite scale, contributing to anaerobic carbon (C) mineralization and changes in soil physical and chemical characteristics, especially the dissolution and precipitation of short-range ordered Fe phases thought to stabilize C. Thus, O2 fluctuations and Fe redox cycling may have multiple nuanced and opposing impacts on different soil C pools, illustrated by recent findings from Fe-rich Oxisols and Ultisols in the Luquillo Experimental Forest, Puerto Rico. Spatial patterns in surface soil C stocks at the landscape scale correlated strongly (R2 = 0.98) with concentrations of reduced Fe (Fe(II)), reflecting constitutive differences in reducing conditions within and among sites that promote C accumulation in mineral soil horizons. Similarly, turnover times of a decadal-cycling pool of mineral-associated organic matter increased with Fe(II) across a catena, possibly reflecting the role of anaerobic microsites in long-term C stabilization. However, two different indices of short-range order Fe showed highly significant opposing relationships (positive and negative) with spatial variation in soil C concentrations, possibly reflecting a dual role of Fe in driving C stabilization via co-precipitation, and C solubilization and loss following dissimilatory Fe reduction. Consistent with the field data, laboratory incubations demonstrated that redox fluctuations can increase the contribution of biochemically recalcitrant C (lignin) to soil respiration, whereas addition of short-range order Fe dramatically suppressed lignin mineralization but had no impact on bulk soil respiration. Thus, understanding spatial and temporal patterns of Fe redox cycling may provide insight into explaining the

  14. Biotite and chlorite weathering at 25 degrees C: the dependence of pH and (bi)carbonate on weathering kinetics, dissolution stoichiometry, and solubility; and the relation to redox conditions in granitic aquifers

    International Nuclear Information System (INIS)

    Malmstroem, M.; Banwart, S.

    1995-01-01

    We have studied the kinetics and thermodynamics of biotite and chlorite weathering in the pH range 2 2 -10 2 year); and 2. the development of characteristic Fe(III) concentrations (10 -5 M in 10 - 1 years). The Fe(III)-bearing clay minerals formed during these experiments are similar to the fracture-filling-material observed at the Aespoe Hard Rock Laboratory. Such clays can provide reducing capacity to a repository. They can help maintain anoxic conditions by consuming oxygen that enters the repository during the construction and operation phases thereby helping maintain the redox stability of the repository regarding canister corrosion. The half-life of oxygen trapped in the repository at the time of closure depends on the rate of oxygen uptake by Fe(II) minerals, sulfide minerals and organic carbon. Fe(II)-clay minerals are important to the redox stability of a repository, as well as providing a sorption barrier to radionuclide migration. 107 refs, 52 figs, 35 tabs

  15. Water table fluctuations and soil biogeochemistry: An experimental approach using an automated soil column system

    Science.gov (United States)

    Rezanezhad, F.; Couture, R.-M.; Kovac, R.; O'Connell, D.; Van Cappellen, P.

    2014-02-01

    Water table fluctuations significantly affect the biological and geochemical functioning of soils. Here, we introduce an automated soil column system in which the water table regime is imposed using a computer-controlled, multi-channel pump connected to a hydrostatic equilibrium reservoir and a water storage reservoir. The potential of this new system is illustrated by comparing results from two columns filled with 45 cm of the same homogenized riparian soil. In one soil column the water table remained constant at -20 cm below the soil surface, while in the other the water table oscillated between the soil surface and the bottom of the column, at a rate of 4.8 cm d-1. The experiment ran for 75 days at room temperature (25 ± 2 °C). Micro-sensors installed at -10 and -30 cm below the soil surface in the stable water table column recorded constant redox potentials on the order of 600 and -200 mV, respectively. In the fluctuating water table column, redox potentials at the same depths oscillated between oxidizing (∼700 mV) and reducing (∼-100 mV) conditions. Pore waters collected periodically and solid-phase analyses on core material obtained at the end of the experiment highlighted striking geochemical differences between the two columns, especially in the time series and depth distributions of Fe, Mn, K, P and S. Soil CO2 emissions derived from headspace gas analysis exhibited periodic variations in the fluctuating water table column, with peak values during water table drawdown. Transient redox conditions caused by the water table fluctuations enhanced microbial oxidation of soil organic matter, resulting in a pronounced depletion of particulate organic carbon in the midsection of the fluctuating water table column. Denaturing Gradient Gel Electrophoresis (DGGE) revealed the onset of differentiation of the bacterial communities in the upper (oxidizing) and lower (reducing) soil sections, although no systematic differences in microbial community structure

  16. Nonequilibrium quantum fluctuations of work.

    Science.gov (United States)

    Allahverdyan, A E

    2014-09-01

    The concept of work is basic for statistical thermodynamics. To gain a fuller understanding of work and its (quantum) features, it needs to be represented as an average of a fluctuating quantity. Here I focus on the work done between two moments of time for a thermally isolated quantum system driven by a time-dependent Hamiltonian. I formulate two natural conditions needed for the fluctuating work to be physically meaningful for a system that starts its evolution from a nonequilibrium state. The existing definitions do not satisfy these conditions due to issues that are traced back to noncommutativity. I propose a definition of fluctuating work that is free of previous drawbacks and that applies for a wide class of nonequilibrium initial states. It allows the deduction of a generalized work-fluctuation theorem that applies for an arbitrary (out-of-equilibrium) initial state.

  17. Redox meets protein trafficking.

    Science.gov (United States)

    Bölter, Bettina; Soll, Jürgen; Schwenkert, Serena

    2015-09-01

    After the engulfment of two prokaryotic organisms, the thus emerged eukaryotic cell needed to establish means of communication and signaling to properly integrate the acquired organelles into its metabolism. Regulatory mechanisms had to evolve to ensure that chloroplasts and mitochondria smoothly function in accordance with all other cellular processes. One essential process is the post-translational import of nuclear encoded organellar proteins, which needs to be adapted according to the requirements of the plant. The demand for protein import is constantly changing depending on varying environmental conditions, as well as external and internal stimuli or different developmental stages. Apart from long-term regulatory mechanisms such as transcriptional/translation control, possibilities for short-term acclimation are mandatory. To this end, protein import is integrated into the cellular redox network, utilizing the recognition of signals from within the organelles and modifying the efficiency of the translocon complexes. Thereby, cellular requirements can be communicated throughout the whole organism. This article is part of a Special Issue entitled: Chloroplast Biogenesis. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Fluctuations in quantum chaos

    International Nuclear Information System (INIS)

    Casati, G.; Chirikov, B.V.

    1996-01-01

    Various fluctuations in quantum systems with discrete spectrum are discussed, including recent unpublished results. Open questions and unexplained peculiarities of quantum fluctuations are formulated [ru

  19. Fluctuations in Cerebral Hemodynamics

    National Research Council Canada - National Science Library

    Latka, Miroslaw

    2003-01-01

    We demonstrate that the scaling properties of intracranial pressure (ICP) fluctuations and fluctuations of blood flow velocity in middle cerebral arteries are characterized by two scaling exponents...

  20. Redox Paradox: A Novel Approach to Therapeutics-Resistant Cancer.

    Science.gov (United States)

    Chaiswing, Luksana; St Clair, William H; St Clair, Daret K

    2018-02-21

    Cancer cells that are resistant to radiation and chemotherapy are a major problem limiting the success of cancer therapy. Aggressive cancer cells depend on elevated intracellular levels of reactive oxygen species (ROS) to proliferate, self-renew, and metastasize. As a result, these aggressive cancers maintain high basal levels of ROS compared with normal cells. The prominence of the redox state in cancer cells led us to consider whether increasing the redox state to the condition of oxidative stress could be used as a successful adjuvant therapy for aggressive cancers. Recent Advances: Past attempts using antioxidant compounds to inhibit ROS levels in cancers as redox-based therapy have met with very limited success. However, recent clinical trials using pro-oxidant compounds reveal noteworthy results, which could have a significant impact on the development of strategies for redox-based therapies. The major objective of this review is to discuss the role of the redox state in aggressive cancers and how to utilize the shift in redox state to improve cancer therapy. We also discuss the paradox of redox state parameters; that is, hydrogen peroxide (H 2 O 2 ) as the driver molecule for cancer progression as well as a target for cancer treatment. Based on the biological significance of the redox state, we postulate that this system could potentially be used to create a new avenue for targeted therapy, including the potential to incorporate personalized redox therapy for cancer treatment. Antioxid. Redox Signal. 00, 000-000.

  1. Redox Species of Redox Flow Batteries: A Review.

    Science.gov (United States)

    Pan, Feng; Wang, Qing

    2015-11-18

    Due to the capricious nature of renewable energy resources, such as wind and solar, large-scale energy storage devices are increasingly required to make the best use of the renewable power. The redox flow battery is considered suitable for large-scale applications due to its modular design, good scalability and flexible operation. The biggest challenge of the redox flow battery is the low energy density. The redox active species is the most important component in redox flow batteries, and the redox potential and solubility of redox species dictate the system energy density. This review is focused on the recent development of redox species. Different categories of redox species, including simple inorganic ions, metal complexes, metal-free organic compounds, polysulfide/sulfur and lithium storage active materials, are reviewed. The future development of redox species towards higher energy density is also suggested.

  2. Impacto dos mediadores redox na remoção de cor de corantes azo e antraquinônico por lodo granular anaeróbio sob condições mesofílicas e termofílicas Impact of redox mediators on colour removal of azo and anthraquinone dyes by anaerobic granular sludge under mesophilic and thermophilic conditions

    Directory of Open Access Journals (Sweden)

    André Bezerra dos Santos

    2007-03-01

    Full Text Available Investigou-se o efeito de diferentes mediadores redox (MR na remoção de cor de corantes azo e antraquinônico pelo uso de lodo granular anaeróbio sob condições mesofílicas (30ºC e termofílicas (55ºC. Comprovou-se em experimento em batelada que a adição de concentrações catalíticas de MR pode ter um efeito marcante nas taxas de descoloração do corante azo Reactive Red 2 (RR2, mas o mesmo comportamento não pode ser obtido com o corante antraquinônico Reactive Blue 5 (RB5. Entretanto, com ambos os corantes, o simples aumento da temperatura de incubação para condições termofílicas fez acelerar consideravelmente os processos de descoloração, comparados com condições mesofílicas. Por exemplo, a constante de primeira ordem "k" da redução dos corantes RR2 e RB5, foi aumentada em 6,2 e 11 vezes, respectivamente, à 55ºC quando comparado com 30ºC. Por fim, comprovou-se em experimentos de fluxo contínuo, a boa performance do tratamento termofílico na descoloração redutiva de corantes azo.The effect of different redox mediators (RM on colour removal of azo and anthraquinone dyes was investigated with anaerobic granular sludge under mesophilic (30ºC and thermophilic (55ºC conditions. Batch experiments revealed that an addition of catalytic concentrations of RM provided a remarkable effect on the decolourisation rates of the azo dye Reactive Red 2 (RR2, but the same effect could not be obtained with the anthraquinone dye Reactive Blue 5 (RB5. Nevertheless, for both dyes, the temperature increase to thermophilic conditions was an effective strategy to considerably accelerate the decolourisation process compared to mesophilic conditions. For instance, the first-order rate constant "k" of RR2 and RB5 reduction, was increased in 6.2 and 11-fold, respectively, at 55ºC in comparison with 30ºC. Such an effect of the temperature on the reductive decolourisation of azo dye was also verified in continuous flow experiments.

  3. Dimensional behavior of Ni-YSZ composites during redox cycling

    DEFF Research Database (Denmark)

    Pihlatie, Mikko; Kaiser, Andreas; Larsen, Peter Halvor

    2009-01-01

    The dimensional behavior of Ni-yttria-stabilized zirconia (YSZ) cermets during redox cycling was tested in dilatometry within the temperature range 600-1000 degrees C. The effect Of humidity oil redox stability was investigated at intermediate and low temperatures. We show that both the sintering...... of nickel depending on temperature of the initial reduction and the operating conditions, and the temperature of reoxidation are very important for the size of the dimensional change. Cumulative redox strain (CRS) is shown to be correlated with temperature. Measured maximum CRS after three redox cycles...

  4. Investigation of activity and selectivity of redox catalysts in oxidative ...

    African Journals Online (AJOL)

    In this study, oxidative coupling of methane on Redox catalysts in fluidized bed reactor was investigated. For this purpose, the catalyst Mn-Na2WO4/SiO2 was selected as a Redox catalyst. In order to investigate this catalyst, transient state experiments were designed and performed. Then, the different reaction conditions on ...

  5. Circadian redox signaling in plant immunity and abiotic stress.

    Science.gov (United States)

    Spoel, Steven H; van Ooijen, Gerben

    2014-06-20

    Plant crops are critically important to provide quality food and bio-energy to sustain a growing human population. Circadian clocks have been shown to deliver an adaptive advantage to plants, vastly increasing biomass production by efficient anticipation to the solar cycle. Plant stress, on the other hand, whether biotic or abiotic, prevents crops from reaching maximum productivity. Stress is associated with fluctuations in cellular redox and increased phytohormone signaling. Recently, direct links between circadian timekeeping, redox fluctuations, and hormone signaling have been identified. A direct implication is that circadian control of cellular redox homeostasis influences how plants negate stress to ensure growth and reproduction. Complex cellular biochemistry leads from perception of stress via hormone signals and formation of reactive oxygen intermediates to a physiological response. Circadian clocks and metabolic pathways intertwine to form a confusing biochemical labyrinth. Here, we aim to find order in this complex matter by reviewing current advances in our understanding of the interface between these networks. Although the link is now clearly defined, at present a key question remains as to what extent the circadian clock modulates redox, and vice versa. Furthermore, the mechanistic basis by which the circadian clock gates redox- and hormone-mediated stress responses remains largely elusive.

  6. How the redox state of tobacco 'Bel-W3' is modified in response to ozone and other environmental factors in a sub-tropical area?

    International Nuclear Information System (INIS)

    Dias, Ana P.L.; Dafre, Marcelle; Rinaldi, Mirian C.S.; Domingos, Marisa

    2011-01-01

    This study intended to determine whether the redox state in plants of Nicotiana tabacum 'Bel-W3' fluctuates in response to the environmental factors in a sub-tropical area contaminated by ozone (Sao Paulo, SE - Brazil) and which environmental factors are related to this fluctuation, discussing their biomonitoring efficiency. We comparatively evaluated the indicators of redox state (ascorbic acid, glutathione, superoxide dismutase, ascorbate peroxidase, and glutathione reductase) and leaf injury in 17 field experiments performed in 2008. The redox state was explained by the combined effects of chronic levels of O 3 and meteorological variables 4-6 days prior to the plant sampling. Moderate leaf injury was observed in most cases. The redox state of tobacco decreases few days after their placement in the sub-tropical environment, causing them to become susceptible to oxidative stress imposed by chronic doses of O 3 . Its bioindicator efficiency would not be diminished in such levels of atmospheric contamination. - Research highlights: → Nicotiana tabacum 'Bel-W3' is potentially a bioindicator of O 3 in the sub-tropics. → However, it is unknown if its redox state would affect its bioindicator performance under sub-tropical environmental conditions. → This study revealed that the redox state of tobacco decreases few days after their placement in the sub-tropical environment, causing them to become susceptible to oxidative stress imposed by chronic doses of O 3 . → Therefore, its bioindicator efficiency would not be diminished in such levels of atmospheric contamination. → However, the bioindicator efficiency N. tabacum 'Bel-W3' for biomonitoring O 3 should be regionally modeled in the sub-tropics, based on both its redox state and on the flux of O 3 through stomata, in response to the varying micro-meteorological conditions that govern both physiological processes. - The bioindicator efficiency of tobacco plants is not restrained under chronic doses of O 3 in

  7. How the redox state of tobacco 'Bel-W3' is modified in response to ozone and other environmental factors in a sub-tropical area?

    Energy Technology Data Exchange (ETDEWEB)

    Dias, Ana P.L.; Dafre, Marcelle; Rinaldi, Mirian C.S. [Instituto de Botanica, Caixa Postal 3005, 01061-970 Sao Paulo, SP (Brazil); Domingos, Marisa, E-mail: mmingos@superig.com.b [Instituto de Botanica, Caixa Postal 3005, 01061-970 Sao Paulo, SP (Brazil)

    2011-02-15

    This study intended to determine whether the redox state in plants of Nicotiana tabacum 'Bel-W3' fluctuates in response to the environmental factors in a sub-tropical area contaminated by ozone (Sao Paulo, SE - Brazil) and which environmental factors are related to this fluctuation, discussing their biomonitoring efficiency. We comparatively evaluated the indicators of redox state (ascorbic acid, glutathione, superoxide dismutase, ascorbate peroxidase, and glutathione reductase) and leaf injury in 17 field experiments performed in 2008. The redox state was explained by the combined effects of chronic levels of O{sub 3} and meteorological variables 4-6 days prior to the plant sampling. Moderate leaf injury was observed in most cases. The redox state of tobacco decreases few days after their placement in the sub-tropical environment, causing them to become susceptible to oxidative stress imposed by chronic doses of O{sub 3}. Its bioindicator efficiency would not be diminished in such levels of atmospheric contamination. - Research highlights: Nicotiana tabacum 'Bel-W3' is potentially a bioindicator of O{sub 3} in the sub-tropics. However, it is unknown if its redox state would affect its bioindicator performance under sub-tropical environmental conditions. This study revealed that the redox state of tobacco decreases few days after their placement in the sub-tropical environment, causing them to become susceptible to oxidative stress imposed by chronic doses of O{sub 3}. Therefore, its bioindicator efficiency would not be diminished in such levels of atmospheric contamination. However, the bioindicator efficiency N. tabacum 'Bel-W3' for biomonitoring O{sub 3} should be regionally modeled in the sub-tropics, based on both its redox state and on the flux of O{sub 3} through stomata, in response to the varying micro-meteorological conditions that govern both physiological processes. - The bioindicator efficiency of tobacco plants is not

  8. Hydrologic conditions and lake-level fluctuations at Long Lost Lake, 1939-2004, White Earth Indian Reservation, Clearwater County, Minnesota

    Science.gov (United States)

    Christensen, Victoria G.; Bergman, Andrea L.

    2005-01-01

    Long Lost Lake, a closed-basin lake in Clearwater County, Minnesota, has had a substantial rise in lake level since 1990. The increased level and surface area of the lake has led to the inundation of nearby homes and roads. The U.S. Geological Survey, in cooperation with the White Earth Band of Chippewa Indians, conducted a study to document the historical lake-level fluctuations, to investigate reasons for hydrologic change, and to develop a general understanding of the hydrology of lakes that have had rapid changes in lake level.

  9. Biotransformation of gabapentin in surface water matrices under different redox conditions and the occurrence of one major TP in the aquatic environment.

    Science.gov (United States)

    Henning, Nina; Kunkel, Uwe; Wick, Arne; Ternes, Thomas A

    2018-06-15

    Laboratory-scale incubation experiments in water/sediment systems were conducted to test the transformation behavior of the anticonvulsant gabapentin (GBP) under different environmental conditions (aerobic, anaerobic, with abiotic controls). GBP was transformed by biological processes as it was eliminated quickly under aerobic conditions (dissipation time 50% of initial concentration (DT 50 ): 2-7 days) whereas no decrease was observed under anaerobic conditions. Measurements via high resolution mass spectrometry (LC-Orbitrap-MS) revealed eight biological transformation products (TPs). Three of them were identified with reference standards (GBP-Lactam, TP186, TP213), while for the other five TPs tentative structures were proposed from information by MS 2 /MS 3 experiments. Furthermore, the quantitatively most relevant TP GBP-Lactam was formed via intramolecular amidation (up to 18% of initial GBP concentration). Incubation experiments with GBP-Lactam revealed a higher stability against biotic degradation (DT 50 : 12 days) in contrast to GBP, while it was stable under anaerobic and abiotic conditions. Besides GBP, GBP-Lactam was detected in surface water in the μg L -1 range. Finally, GBP and GBP-Lactam were found in potable water with concentrations up to 0.64 and 0.07 μg L -1 , respectively. According to the elevated environmental persistence of GBP-Lactam compared to GBP and its presumed enhanced toxicity, we recommend to involve GBP-Lactam into monitoring programs. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. Measurement of Redox Potential in Nanoecotoxicological Investigations

    Directory of Open Access Journals (Sweden)

    Ratna Tantra

    2012-01-01

    Full Text Available Redox potential has been identified by the Organisation for Economic Co-operation and Development (OECD as one of the parameters that should be investigated for the testing of manufactured nanomaterials. There is still some ambiguity concerning this parameter, i.e., as to what and how to measure, particularly when in a nanoecotoxicological context. In this study the redox potentials of six nanomaterials (either zinc oxide (ZnO or cerium oxide (CeO2 dispersions were measured using an oxidation-reduction potential (ORP electrode probe. The particles under testing differed in terms of their particle size and dispersion stability in deionised water and in various ecotox media. The ORP values of the various dispersions and how they fluctuate relative to each other are discussed. Results show that the ORP values are mainly governed by the type of liquid media employed, with little contributions from the nanoparticles. Seawater was shown to have reduced the ORP value, which was attributed to an increase in the concentration of reducing agents such as sulphites or the reduction of dissolved oxygen concentration. The lack of redox potential value contribution from the particles themselves is thought to be due to insufficient interaction of the particles at the Pt electrode of the ORP probe.

  11. Measurement of redox potential in nanoecotoxicological investigations.

    Science.gov (United States)

    Tantra, Ratna; Cackett, Alex; Peck, Roger; Gohil, Dipak; Snowden, Jacqueline

    2012-01-01

    Redox potential has been identified by the Organisation for Economic Co-operation and Development (OECD) as one of the parameters that should be investigated for the testing of manufactured nanomaterials. There is still some ambiguity concerning this parameter, i.e., as to what and how to measure, particularly when in a nanoecotoxicological context. In this study the redox potentials of six nanomaterials (either zinc oxide (ZnO) or cerium oxide (CeO(2))) dispersions were measured using an oxidation-reduction potential (ORP) electrode probe. The particles under testing differed in terms of their particle size and dispersion stability in deionised water and in various ecotox media. The ORP values of the various dispersions and how they fluctuate relative to each other are discussed. Results show that the ORP values are mainly governed by the type of liquid media employed, with little contributions from the nanoparticles. Seawater was shown to have reduced the ORP value, which was attributed to an increase in the concentration of reducing agents such as sulphites or the reduction of dissolved oxygen concentration. The lack of redox potential value contribution from the particles themselves is thought to be due to insufficient interaction of the particles at the Pt electrode of the ORP probe.

  12. Separate effects of flooding and anaerobiosis on soil greenhouse gas emissions and redox sensitive biogeochemistry

    Science.gov (United States)

    Gavin McNicol; Whendee L. Silver

    2014-01-01

    Soils are large sources of atmospheric greenhouse gases, and both the magnitude and composition of soil gas emissions are strongly controlled by redox conditions. Though the effect of redox dynamics on greenhouse gas emissions has been well studied in flooded soils, less research has focused on redox dynamics without total soil inundation. For the latter, all that is...

  13. Redox Flow Batteries, a Review

    Energy Technology Data Exchange (ETDEWEB)

    Knoxville, U. Tennessee; U. Texas Austin; U, McGill; Weber, Adam Z.; Mench, Matthew M.; Meyers, Jeremy P.; Ross, Philip N.; Gostick, Jeffrey T.; Liu, Qinghua

    2011-07-15

    Redox flow batteries are enjoying a renaissance due to their ability to store large amounts of electrical energy relatively cheaply and efficiently. In this review, we examine the components of redox flow batteries with a focus on understanding the underlying physical processes. The various transport and kinetic phenomena are discussed along with the most common redox couples.

  14. Transition from confined to phreatic conditions as the factor controlling salinization and change in redox state, Upper subaquifer of the Judea Group, Israel

    Science.gov (United States)

    Gavrieli, Ittai; Burg, Avi; Guttman, Joseph

    2002-08-01

    An increase in salinity and change from oxic to anoxic conditions are observed in the Upper subaquifer of the Judea Group in the Kefar Uriyya pumping field at the western foothills of the Judea Mountains, Israel. Hydrogeological data indicate that the change, which occurs over a distance of only a few kilometers, coincides with a transition from confined to phreatic conditions in the aquifer. The deterioration in the water quality is explained as a result of seepage of more saline, organic-rich water from above, into the phreatic "roofed" part of the aquifer. The latter is derived from the bituminous chalky rocks of the Mount Scopus Group, which confine the aquifer in its southeastern part. In this confined part, water in perched horizons within the Mount Scopus Group cannot leak down and flow westward while leaching organic matter and accumulating salts. However, upon reaching the transition area from confined to phreatic conditions, seepage to the Judea Upper subaquifer is possible, thereby allowing it to be defined as a leaky aquifer. The incoming organic matter consumes the dissolved oxygen and allows bacterial sulfate reduction. The latter accounts for the H2S in the aquifer, as indicated by sulfur isotopic analyses of coexisting sulfate and sulfide. Thus, from an aquifer management point of view, in order to maintain the high quality of the water in the confined southeastern part of the Kefar Uriyya field, care should be taken not to draw the confined-roofed transition area further east by over pumping.

  15. Fluctuations in LC Oscillators

    Directory of Open Access Journals (Sweden)

    O. Ondracek

    1994-03-01

    Full Text Available An analysis of the phase and amplitude fluctuations in oscillators with simple resonant circuit is presented. Negative feedback is used to minimize effect of the inherent noise produced by bipolar transistor on fluctuation characteristics.

  16. Intense molybdenum accumulation in sediments underneath a nitrogenous water column and implications for the reconstruction of paleo-redox conditions based on molybdenum isotopes

    Science.gov (United States)

    Scholz, Florian; Siebert, Christopher; Dale, Andrew W.; Frank, Martin

    2017-09-01

    The concentration and isotope composition of molybdenum (Mo) in sediments and sedimentary rocks are widely used proxies for anoxic conditions in the water column of paleo-marine systems. While the mechanisms leading to Mo fixation in modern restricted basins with anoxic and sulfidic (euxinic) conditions are reasonably well constrained, few studies have focused on Mo cycling in the context of open-marine anoxia. Here we present Mo data for water column particulate matter, modern surface sediments and a paleo-record covering the last 140,000 years from the Peruvian continental margin. Mo concentrations in late Holocene and Eemian (penultimate interglacial) shelf sediments off Peru range from ∼70 to 100 μg g-1, an extent of Mo enrichment that is thought to be indicative of (and limited to) euxinic systems. To investigate if this putative anomaly could be related to the occasional occurrence of sulfidic conditions in the water column overlying the Peruvian shelf, we compared trace metal (Mo, vanadium, uranium) enrichments in particulate matter from oxic, nitrate-reducing (nitrogenous) and sulfidic water masses. Coincident enrichments of iron (Fe) (oxyhydr)oxides and Mo in the nitrogenous water column as well as co-variation of dissolved Fe and Mo in the sediment pore water suggest that Mo is delivered to the sediment surface by Fe (oxyhydr)oxides. Most of these precipitate in the anoxic-nitrogenous water column due to oxidation of sediment-derived dissolved Fe with nitrate as a terminal electron acceptor. Upon reductive dissolution in the surface sediment, a fraction of the Fe and Mo is re-precipitated through interaction with pore water sulfide. The Fe- and nitrate-dependent mechanism of Mo accumulation proposed here is supported by the sedimentary Mo isotope composition, which is consistent with Mo adsorption onto Fe (oxyhydr)oxides. Trace metal co-variation patterns as well as Mo and nitrogen isotope systematics suggest that the same mechanism of Mo delivery

  17. Changes in productivity and redox conditions during the Last Glacial Maximum as recorded in high-resolution geochemical records from Alfonso Basin, Gulf of California

    Science.gov (United States)

    Choumiline, K.; Lyons, T. W.; Carriquiry, J. D.; Perez-Cruz, L. L.; Beaufort, L.

    2015-12-01

    The Last Glacial Maximum represents the most recent major sea level low stand in Earth history. Such changes in eustatic sea level impacted the oxygenation of marine basins, yet the characteristics and mechanisms of those impacts remain poorly known. Specific basin conditions are needed to develop, record, and preserve those paleoredox changes through time most effectively, including rapid sedimentation rates and silled basin morphologies. The anoxic Alfonso Basin, partially separated from the Gulf of California by a bathymetric sill, is such a place. The basin is located in a dry semiarid region dominated by dust inputs and only occasional tropical cyclone-induced fluvial contributions. We present the first multi-proxy reconstruction of southern Gulf of California paleoredox that covers an uninterrupted timespan from the Late Pleistocene to the present, with an emphasis on the Last Glacial Maximum. In this research we contrast geochemical data from a 47-meter-long sediment core (collected with the giant CALYPSO corer aboard the R/V Marion Dufresne) with the solid phase and pore water chemical data from a shorter but very detailed box core (collected on the R/V El Puma), both from Alfonso Basin. Our results show that during the Late Pleistocene and throughout the Holocene several paleoredox shifts occurred, often accompanied by differences in lamination (laminated/massive alternations) and trace element (Mo, V, U) compositions; detailed Fe chemistry (FeHR/FeTand Fe/Al ratios); as well as carbon and sulfur concentrations and isotope ratios. For example, FeHR/FeT ratios indicate values of roughly 0.15 during the Last Glacial Maximum (lower than theoretic threshold of 0.2 between oxic and anoxic), suggesting more oxic conditions, in comparison to 0.25 and higher in the Holocene. Most of the variability seen in these proxies is related to global sea level change, while some variability is instead related to local variations in paleoproductivity, often connected to

  18. Salt stress affects the redox status of Arabidopsis root meristems

    Directory of Open Access Journals (Sweden)

    Keni eJiang

    2016-02-01

    Full Text Available We report the redox status (profiles for specific populations of cells that comprise the Arabidopsis root tip. For recently germinated, 3-5-day-old seedlings we show that the region of the root tip with the most reduced redox status includes the root cap initials, the quiescent center and the most distal portion of the proximal meristem, and coincides with (overlays the region of the auxin maximum. As one moves basally, further into the proximal meristem, and depending on the growth conditions, the redox status becomes more oxidized, with a 5-10 mV difference in redox potential between the two borders delimiting the proximal meristem. At the point on the root axis at which cells of the proximal meristem cease division and enter the transition zone, the redox potential levels off and remains more or less unchanged throughout the transition zone. As cells leave the transition zone and enter the zone of elongation the redox potentials become more oxidized. Treating roots with salt (50, 100 and 150 mM NaCl results in marked changes in root meristem structure and development, and is preceded by changes in the redox profile, which flattens, and initially becomes more oxidized, with pronounced changes in the redox potentials of the root cap, the root cap initials and the quiescent center. Roots exposed to relatively mild levels of salt (< 100 mM are able to re-establish a normal, pre-salt treatment redox profile 3-6 days after exposure to salt. Coincident with the salt-associated changes in redox profiles are changes in the distribution of auxin transporters (AUX1, PIN1/2, which become more diffuse in their localization. We conclude that salt stress affects root meristem maintenance, in part, through changes in redox and auxin transport.

  19. Redox-capacitor to connect electrochemistry to redox-biology.

    Science.gov (United States)

    Kim, Eunkyoung; Leverage, W Taylor; Liu, Yi; White, Ian M; Bentley, William E; Payne, Gregory F

    2014-01-07

    It is well-established that redox-reactions are integral to biology for energy harvesting (oxidative phosphorylation), immune defense (oxidative burst) and drug metabolism (phase I reactions), yet there is emerging evidence that redox may play broader roles in biology (e.g., redox signaling). A critical challenge is the need for tools that can probe biologically-relevant redox interactions simply, rapidly and without the need for a comprehensive suite of analytical methods. We propose that electrochemistry may provide such a tool. In this tutorial review, we describe recent studies with a redox-capacitor film that can serve as a bio-electrode interface that can accept, store and donate electrons from mediators commonly used in electrochemistry and also in biology. Specifically, we (i) describe the fabrication of this redox-capacitor from catechols and the polysaccharide chitosan, (ii) discuss the mechanistic basis for electron exchange, (iii) illustrate the properties of this redox-capacitor and its capabilities for promoting redox-communication between biology and electrodes, and (iv) suggest the potential for enlisting signal processing strategies to "extract" redox information. We believe these initial studies indicate broad possibilities for enlisting electrochemistry and signal processing to acquire "systems level" redox information from biology.

  20. Redox-Based Regulation of Bacterial Development and Behavior.

    Science.gov (United States)

    Sporer, Abigail J; Kahl, Lisa J; Price-Whelan, Alexa; Dietrich, Lars E P

    2017-06-20

    Severe changes in the environmental redox potential, and resulting alterations in the oxidation states of intracellular metabolites and enzymes, have historically been considered negative stressors, requiring responses that are strictly defensive. However, recent work in diverse organisms has revealed that more subtle changes in the intracellular redox state can act as signals, eliciting responses with benefits beyond defense and detoxification. Changes in redox state have been shown to influence or trigger chromosome segregation, sporulation, aerotaxis, and social behaviors, including luminescence as well as biofilm establishment and dispersal. Connections between redox state and complex behavior allow bacteria to link developmental choices with metabolic state and coordinate appropriate responses. Promising future directions for this area of study include metabolomic analysis of species- and condition-dependent changes in metabolite oxidation states and elucidation of the mechanisms whereby the redox state influences circadian regulation.

  1. Hydrodynamical fluctuations in smooth shear flows

    International Nuclear Information System (INIS)

    Chagelishvili, G.D.; Khujadze, G.R.; Lominadze, J.G.

    1999-11-01

    Background of hydrodynamical fluctuations in a intrinsically/stochastically forced, laminar, uniform shear flow is studied. The employment of so-called nonmodal mathematical analysis makes it possible to represent the background of fluctuations in a new light and to get more insight into the physics of its formation. The basic physical processes responsible for the formation of vortex and acoustic wave fluctuation backgrounds are analyzed. Interplay of the processes at low and moderate shear rates is described. Three-dimensional vortex fluctuations around a given macroscopic state are numerically calculated. The correlation functions of the fluctuations of physical quantities are analyzed. It is shown that there exists subspace D k in the wave-number space (k-space) that is limited externally by spherical surface with radius k ν ≡ A/ν (where A is the velocity shear parameter, ν - the kinematic viscosity) in the nonequilibrium open system under study. The spatial Fourier harmonics of vortex as well as acoustic wave fluctuations are strongly subjected by flow shear (by the open character of the system) at wave-numbers satisfying the condition k ν . Specifically it is shown that in D k : The fluctuations are non-Markovian; the spatial spectral density of energy of the vortex fluctuations by far exceeds the white-noise; the term of a new type associated to the hydrodynamical fluctuation of velocity appears in the correlation function of pressure; the fluctuation background of the acoustic waves is completely different at low and moderate shear rates (at low shear rates it is reduced in D k in comparison to the uniform (non-shear) flow; at moderate shear rates it it comparable to the background of the vortex fluctuations). The fluctuation background of both the vortex and the acoustic wave modes is anisotropic. The possible significance of the fluctuation background of vortices for the subcritical transition to turbulence and Brownian motion of small macroscopic

  2. Determining redox properties of clay-rich sedimentary deposits in the context of performance assessment of radioactive waste repositories : Conceptual and practical aspects

    NARCIS (Netherlands)

    Behrends, T.; Bruggeman, Christophe

    Redox reactions play a key factor controlling the mobility of redox sensitive radionuclides in clay-rich sediments which might serve as host formations for radioactive waste repositories. Assessing the redox speciation of radionuclides requires information about the redox conditions in the formation

  3. Redox-active nanomaterials for nanomedicine applications.

    Science.gov (United States)

    Sims, Christopher M; Hanna, Shannon K; Heller, Daniel A; Horoszko, Christopher P; Johnson, Monique E; Montoro Bustos, Antonio R; Reipa, Vytas; Riley, Kathryn R; Nelson, Bryant C

    2017-10-19

    Nanomedicine utilizes the remarkable properties of nanomaterials for the diagnosis, treatment, and prevention of disease. Many of these nanomaterials have been shown to have robust antioxidative properties, potentially functioning as strong scavengers of reactive oxygen species. Conversely, several nanomaterials have also been shown to promote the generation of reactive oxygen species, which may precipitate the onset of oxidative stress, a state that is thought to contribute to the development of a variety of adverse conditions. As such, the impacts of nanomaterials on biological entities are often associated with and influenced by their specific redox properties. In this review, we overview several classes of nanomaterials that have been or projected to be used across a wide range of biomedical applications, with discussion focusing on their unique redox properties. Nanomaterials examined include iron, cerium, and titanium metal oxide nanoparticles, gold, silver, and selenium nanoparticles, and various nanoscale carbon allotropes such as graphene, carbon nanotubes, fullerenes, and their derivatives/variations. Principal topics of discussion include the chemical mechanisms by which the nanomaterials directly interact with biological entities and the biological cascades that are thus indirectly impacted. Selected case studies highlighting the redox properties of nanomaterials and how they affect biological responses are used to exemplify the biologically-relevant redox mechanisms for each of the described nanomaterials.

  4. Tuning of redox regulatory mechanisms, reactive oxygen species and redox homeostasis under salinity stress

    Directory of Open Access Journals (Sweden)

    Hossain eSazzad

    2016-05-01

    Full Text Available Soil salinity is a crucial environmental constraint which limits biomass production at many sites on a global scale. Saline growth conditions cause osmotic and ionic imbalances, oxidative stress and perturb metabolism, e.g. the photosynthetic electron flow. The plant ability to tolerate salinity is determined by multiple biochemical and physiological mechanisms protecting cell functions, in particular by regulating proper water relations and maintaining ion homeostasis. Redox homeostasis is a fundamental cell property. Its regulation includes control of reactive oxygen species (ROS generation, sensing deviation from and readjustment of the cellular redox state. All these redox related functions have been recognized as decisive factors in salinity acclimation and adaptation. This review focuses on the core response of plants to overcome the challenges of salinity stress through regulation of ROS generation and detoxification systems and to maintain redox homeostasis. Emphasis is given to the role of NADH oxidase (RBOH, alternative oxidase (AOX, the plastid terminal oxidase (PTOX and the malate valve with the malate dehydrogenase isoforms under salt stress. Overwhelming evidence assigns an essential auxiliary function of ROS and redox homeostasis to salinity acclimation of plants.

  5. An Excel Workbook for Identifying Redox Processes in Ground Water

    Science.gov (United States)

    Jurgens, Bryant C.; McMahon, Peter B.; Chapelle, Francis H.; Eberts, Sandra M.

    2009-01-01

    The reduction/oxidation (redox) condition of ground water affects the concentration, transport, and fate of many anthropogenic and natural contaminants. The redox state of a ground-water sample is defined by the dominant type of reduction/oxidation reaction, or redox process, occurring in the sample, as inferred from water-quality data. However, because of the difficulty in defining and applying a systematic redox framework to samples from diverse hydrogeologic settings, many regional water-quality investigations do not attempt to determine the predominant redox process in ground water. Recently, McMahon and Chapelle (2008) devised a redox framework that was applied to a large number of samples from 15 principal aquifer systems in the United States to examine the effect of redox processes on water quality. This framework was expanded by Chapelle and others (in press) to use measured sulfide data to differentiate between iron(III)- and sulfate-reducing conditions. These investigations showed that a systematic approach to characterize redox conditions in ground water could be applied to datasets from diverse hydrogeologic settings using water-quality data routinely collected in regional water-quality investigations. This report describes the Microsoft Excel workbook, RedoxAssignment_McMahon&Chapelle.xls, that assigns the predominant redox process to samples using the framework created by McMahon and Chapelle (2008) and expanded by Chapelle and others (in press). Assignment of redox conditions is based on concentrations of dissolved oxygen (O2), nitrate (NO3-), manganese (Mn2+), iron (Fe2+), sulfate (SO42-), and sulfide (sum of dihydrogen sulfide [aqueous H2S], hydrogen sulfide [HS-], and sulfide [S2-]). The logical arguments for assigning the predominant redox process to each sample are performed by a program written in Microsoft Visual Basic for Applications (VBA). The program is called from buttons on the main worksheet. The number of samples that can be analyzed

  6. Direct electrochemistry of redox proteins

    NARCIS (Netherlands)

    Heering, H.A.

    1995-01-01

    The goal of the project was to obtain more detailed insight in interactions between redox proteins and solid electrodes and the mechanisms of electron transfer. In addition to this, the influence of the protein environment on the redox properties of the active site and the possible

  7. Wind fluctuations over the North Sea

    DEFF Research Database (Denmark)

    Vincent, Claire Louise; Pinson, Pierre; Giebel, Gregor

    2011-01-01

    Climatological patterns in wind speed fluctuations with periods of 1 min to 10 h are analysed using data from a meteorological mast in the Danish North Sea. Fluctuations on these time scales are of particular relevance to the effective management of the power supply from large wind farms. The Hil......Climatological patterns in wind speed fluctuations with periods of 1 min to 10 h are analysed using data from a meteorological mast in the Danish North Sea. Fluctuations on these time scales are of particular relevance to the effective management of the power supply from large wind farms...... a certain class of conditions can be found. Here, the HHT is applied to create conditional spectra which demonstrate patterns in the occurrence of severe wind variability. It is shown that wind fluctuations over the North Sea are more severe for westerly flow than for easterly flow, and that severe...... fluctuations are often observed in the vicinity of precipitation. The most severe wind fluctuations occur in the autumn and winter seasons, and are slightly more common when the pressure tendency is rising. Further, it is found that the wind is more variable for atmospherically unstable conditions than...

  8. Mo isotopes as redox indicators for the Southern Tethys during the PETM

    Science.gov (United States)

    Wouters, H.; Dickson, A.; Porcelli, D.; Hesselbo, S. P.; van den Boorn, S.; Gomez, V. G.; Mutterlose, J.

    2014-12-01

    As several ocean and climate models predict a decline in dissolved ocean oxygen concentrations associated with future global warming [1], recent research is increasingly focusing on past episodes of low ocean oxygen levels. Trace metals are generally enriched in organic-rich sediments deposited under such low oxygen conditions, and the concentration and isotopic signatures of several of these elements (e.g. Mo, U, Cr) may be applied as proxies to reconstruct the processes involved in these redox changes [2,3]. This project investigates the use of the molybdenum isotope system as a proxy for redox changes during an interval of abrupt environmental change spanning the Paleocene/Eocene boundary (the Paleocene/Eocene Thermal Maximum, PETM, ~56 Ma). The PETM is characterized by global warming and environmental and ecological changes including decreased ocean oxygen levels [4]. Study of the PETM can therefore offer us a valuable insight into how marine ecosystems and biogeochemical cycles may respond to future climate changes, and the predicted decrease of oxygen concentrations in seawater. The molybdenum concentrations and isotope compositions of organic-rich sediments spanning the PETM have been obtained from a Jordan oil shale drill core (OS-28). The obtained δ98/95Mo isotopic ratios range between -0.12‰ and 1.59‰ and coincide with significant fluctuations in trace metal abundances. The data together demonstrate that the global environmental changes associated with the PETM were manifest in the Jordanian basin as significant changes in basin hydrography and dissolved oxygen levels.

  9. Demographic response of cutlassfish (Trichiurus japonicus and T. nanhaiensis) to fluctuating palaeo-climate and regional oceanographic conditions in the China seas.

    Science.gov (United States)

    He, Lijun; Zhang, Aibing; Weese, David; Li, Shengfa; Li, Jiansheng; Zhang, Jing

    2014-09-16

    Glacial cycles of the Quaternary have heavily influenced the demographic history of various species. To test the evolutionary impact of palaeo-geologic and climatic events on the demographic history of marine taxa from the coastal Western Pacific, we investigated the population structure and demographic history of two economically important fish (Trichiurus japonicus and T. nanhaiensis) that inhabit the continental shelves of the East China and northern South China Seas using the mitochondrial cytochrome b sequences and Bayesian Skyline Plot analyses. A molecular rate of 2.03% per million years, calibrated to the earliest flooding of the East China Sea shelf (70-140 kya), revealed a strong correlation between population sizes and primary production. Furthermore, comparison of the demographic history of T. japonicus populations from the East China and South China Seas provided evidence of the postglacial development of the Changjiang (Yangtze River) Delta. In the South China Sea, interspecific comparisons between T. japonicus and T. nanhaiensis indicated possible evolutionary responses to changes in palaeo-productivity that were influenced by East Asian winter monsoons. This study not only provides insight into the demographic history of cutlassfish but also reveals potential clues regarding the historic productivity and regional oceanographic conditions of the Western Pacific marginal seas.

  10. Demographic response of cutlassfish (Trichiurus japonicus and T. nanhaiensis) to fluctuating palaeo-climate and regional oceanographic conditions in the China seas

    Science.gov (United States)

    He, Lijun; Zhang, Aibing; Weese, David; Li, Shengfa; Li, Jiansheng; Zhang, Jing

    2014-01-01

    Glacial cycles of the Quaternary have heavily influenced the demographic history of various species. To test the evolutionary impact of palaeo-geologic and climatic events on the demographic history of marine taxa from the coastal Western Pacific, we investigated the population structure and demographic history of two economically important fish (Trichiurus japonicus and T. nanhaiensis) that inhabit the continental shelves of the East China and northern South China Seas using the mitochondrial cytochrome b sequences and Bayesian Skyline Plot analyses. A molecular rate of 2.03% per million years, calibrated to the earliest flooding of the East China Sea shelf (70–140 kya), revealed a strong correlation between population sizes and primary production. Furthermore, comparison of the demographic history of T. japonicus populations from the East China and South China Seas provided evidence of the postglacial development of the Changjiang (Yangtze River) Delta. In the South China Sea, interspecific comparisons between T. japonicus and T. nanhaiensis indicated possible evolutionary responses to changes in palaeo-productivity that were influenced by East Asian winter monsoons. This study not only provides insight into the demographic history of cutlassfish but also reveals potential clues regarding the historic productivity and regional oceanographic conditions of the Western Pacific marginal seas. PMID:25223336

  11. Multiple redox states of multiheme cytochromes may enable bacterial response to changing redox environments

    Science.gov (United States)

    Arbour, T.; Wrighton, K. C.; Mullin, S. W.; Castelle, C.; Luef, B.; Gilbert, B.; Banfield, J. F.

    2013-12-01

    Multiheme c-type cytochromes (MHCs) are key components in electron-transport pathways that enable some microorganisms to transfer electron byproducts of metabolism to a variety of minerals. As a response to changes in mineral redox potential, microbial communities may shift their membership, or individual organisms may adjust protein expression. Alternatively, the ability to respond may be conferred by the innate characteristics of certain electron-transport-chain components. Here, we used potentiostat-controlled microbial fuel cells (MFCs) to measure the timescale of response to imposed changes in redox conditions, thus placing constraints on the importance of these different mechanisms. In the experiments, a solid electrode acts as an electron-accepting mineral whose redox potential can be precisely controlled. We inoculated duplicate MFCs with a sediment/groundwater mixture from an aquifer at Rifle, Colorado, supplied acetate as an electron donor, and obtained stable, mixed-species biofilms dominated by Geobacter and a novel Geobacter-related family. We poised the anode at potentials spanning the range of natural Fe(III)-reduction, then performed cyclic voltammetry (CV) to characterize the overall biofilm redox signature. The apparent biofilm midpoint potential shifted directly with anode set potential when the latter was changed within the range from about -250 to -50 mV vs. SHE. Following a jump in set potential by 200 mV, the CV-midpoint shift by ~100 mV over a timescale of ~30 minutes to a few hours, depending on the direction of the potential change. The extracellular electron transfer molecules, whose overall CV signature is very similar to those of purified MHCs, appear to span a broad redox range (~200 mV), supporting the hypothesis that MHCs confer substantial redox flexibility. This flexibility may be a principle reason for the abundance of MHCs expressed by microorganisms capable of extracellular electron transfer to minerals.

  12. Quantum fluctuations and inflation

    International Nuclear Information System (INIS)

    Bardeen, J.M.; Bublik, G.J.

    1987-01-01

    The authors study the effect of quantum fluctuations on the roll-down rate of the inflation field in a semiclassical approximation; this is done by treating the inflation field as a classical random field. The quantum fluctuations are simulated by a noise term in the equation of motion. Two different inflationary scenarios (new and chaotic inflation) are considered and it is found that the roll-down rate of the median value of the inflation field is increased by the quantum fluctuations. Non-linear effects may become important in the later stages of the inflationary regime. (author)

  13. Fully Quantum Fluctuation Theorems

    Directory of Open Access Journals (Sweden)

    Johan Åberg

    2018-02-01

    Full Text Available Systems that are driven out of thermal equilibrium typically dissipate random quantities of energy on microscopic scales. Crooks fluctuation theorem relates the distribution of these random work costs to the corresponding distribution for the reverse process. By an analysis that explicitly incorporates the energy reservoir that donates the energy and the control system that implements the dynamic, we obtain a quantum generalization of Crooks theorem that not only includes the energy changes in the reservoir but also the full description of its evolution, including coherences. Moreover, this approach opens up the possibility for generalizations of the concept of fluctuation relations. Here, we introduce “conditional” fluctuation relations that are applicable to nonequilibrium systems, as well as approximate fluctuation relations that allow for the analysis of autonomous evolution generated by global time-independent Hamiltonians. We furthermore extend these notions to Markovian master equations, implicitly modeling the influence of the heat bath.

  14. Fishing and stock fluctuations

    National Research Council Canada - National Science Library

    Laevastu, Taivo; Favorite, F

    1988-01-01

    .... Scarcely publicized are the multitude of causes of fish stock fluctuations. This book attempts to summarize the available knowledge on the subject and includes original work of the authors on a matter vital to the fisheries industries of the world...

  15. Classical and quantum temperature fluctuations via holography

    Energy Technology Data Exchange (ETDEWEB)

    Balatsky, Alexander [Nordita, KTH Royal Institute of Technology and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); Institute for Materials Science, Los Alamos National Laboratory,Los Alamos, NM 87545 (United States); Gudnason, Sven Bjarke; Kedem, Yaron [Nordita, KTH Royal Institute of Technology and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); Krikun, Alexander [Nordita, KTH Royal Institute of Technology and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); Institute of Theoretical and Experimental Physics,B. Cheremushkinskaya 25, 117218 Moscow (Russian Federation); Thorlacius, Lárus [Nordita, KTH Royal Institute of Technology and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); University of Iceland, Science Institute,Dunhaga 3, IS-107 Reykjavik (Iceland); The Oskar Klein Centre for Cosmoparticle Physics, Department of Physics,Stockholm University, AlbaNova University Centre, 10691 Stockholm (Sweden); Zarembo, Konstantin [Nordita, KTH Royal Institute of Technology and Stockholm University,Roslagstullsbacken 23, SE-106 91 Stockholm (Sweden); Institute of Theoretical and Experimental Physics,B. Cheremushkinskaya 25, 117218 Moscow (Russian Federation); Department of Physics and Astronomy, Uppsala University,SE-751 08 Uppsala (Sweden)

    2015-01-07

    We study local temperature fluctuations in a 2+1 dimensional CFT on the sphere, dual to a black hole in asymptotically AdS spacetime. The fluctuation spectrum is governed by the lowest-lying hydrodynamic modes of the system whose frequency and damping rate determine whether temperature fluctuations are thermal or quantum. We calculate numerically the corresponding quasinormal frequencies and match the result with the hydrodynamics of the dual CFT at high temperature. As a by-product of our analysis we determine the appropriate boundary conditions for calculating low-lying quasinormal modes for a four-dimensional Reissner-Nordström black hole in global AdS.

  16. Scaling metabolic rate fluctuations

    OpenAIRE

    Labra, Fabio A.; Marquet, Pablo A.; Bozinovic, Francisco

    2007-01-01

    Complex ecological and economic systems show fluctuations in macroscopic quantities such as exchange rates, size of companies or populations that follow non-Gaussian tent-shaped probability distributions of growth rates with power-law decay, which suggests that fluctuations in complex systems may be governed by universal mechanisms, independent of particular details and idiosyncrasies. We propose here that metabolic rate within individual organisms may be considered as an example of an emerge...

  17. Redox potential - field measurements - meassured vs. expected values

    Science.gov (United States)

    Stavělová, Monika; Kovář, Martin

    2016-04-01

    Oxidation and reduction (redox) potential is an important and theoretically very well defined parameter and can be calculated accurately. Its value is determinative for management of many electrochemical processes, chemical redox technologies as well as biotechnologies. To measure the redox value that would correspond with the accuracy level of theoretical calculations in field or operational conditions is however nearly impossible. Redox is in practice measured using combined argentochloride electrode with subsequent value conversion to standard hydrogen electrode (EH). Argentochloride electrode does not allow for precise calibration. Prior to the measurement the accuracy of measurement of particular electrode can only be verified in comparative/control solution with value corresponding with oxic conditions (25°C: +220 mV argentochloride electrode, i.e.. +427 mV after conversion to EH). A commercial product of stabile comparative solution for anoxic conditions is not available and therefore not used in every day practice - accuracy of negative redox is not verified. In this presentation results of two tests will be presented: a) monitoring during dynamic groundwater sampling from eight monitoring wells at a site contaminated by chlorinated ethenes (i.e. post-oxic to anoxic conditions) and b) laboratory test of groundwater contaminated by arsenic from two sites during reaction with highly oxidized compounds of iron (ferrates) - i.e. strongly oxic conditions. In both tests a simultaneous measurement by four argentochloride electrodes was implemented - all four electrodes were prior to the test maintained expertly. The redox values of testing electrodes in a comparative solution varied by max. 6 mV. The redox values measured by four electrodes in both anoxic and oxic variant varied by tens to a hundred mV, while with growing time of test the variance of measured redox values increased in both oxic and anoxic variant. Therefore the interpretation of measured redox

  18. Redox Properties of Free Radicals.

    Science.gov (United States)

    Neta, P.

    1981-01-01

    Describes pulse radiolysis as a useful means in studing one-electron redox potentials. This method allows the production of radicals and the determination of their concentration and rates of reaction. (CS)

  19. Primordial fluctuations without scalar fields

    Science.gov (United States)

    Magueijo, João; Noller, Johannes

    2010-02-01

    We revisit the question of whether fluctuations in hydrodynamical, adiabatical matter could explain the observed structures in our Universe. We consider matter with variable equation of state w=p0/ɛ0 and a concomitant (under the adiabatic assumption) density dependent speed of sound, cs. We find a limited range of possibilities for a setup when modes start inside the Hubble radius, then leaving it and freezing out. For expanding universes, power-law w(ɛ0) models are ruled out (except when cs2∝w≪1, requiring post-stretching the seeded fluctuations); but sharper profiles in cs do solve the horizon problem. Among these, a phase transition in cs is notable for leading to scale-invariant fluctuations if the initial conditions are thermal. For contracting universes all power-law w(ɛ0) solve the horizon problem, but only one leads to scale-invariance: w∝ɛ02 and cs∝ɛ0. This model bypasses a number of problems with single scalar field cyclic models (for which w is large but constant).

  20. Redox regulation of protein damage in plasma

    Directory of Open Access Journals (Sweden)

    Helen R. Griffiths

    2014-01-01

    In this review, we focus on redox regulatory control of those enzymes and processes which control protein maturation during synthesis, produce reactive species, repair and remove damaged plasma proteins. We have highlighted the potential for alterations in the extracellular redox compartment to regulate intracellular redox state and, conversely, for intracellular oxidative stress to alter the cellular secretome and composition of extracellular vesicles. Through secreted, redox-active regulatory molecules, changes in redox state may be transmitted to distant sites.

  1. Universal mesoscopic conductance fluctuations

    International Nuclear Information System (INIS)

    Evangelou, S.N.

    1992-01-01

    The theory of conductance fluctuations in disordered metallic systems with size large compared to the mean free path of the electron but small compared to localization length is considered. It is demonstrates that fluctuations have an universal character and are due to repulsion between levels and spectral rigidity. The basic fluctuation measures for the energy spectrum in the mesoscopic regime of disordered systems are consistent with the Gaussian random matrix ensemble predictions. Although our disordered electron random matrix ensemble does not belong to the Gaussian ensemble the two ensembles turn out to be essentially similar. The level repulsion and the spectral rigidity found in nuclear spectra should also be observed in the metallic regime of Anderson localization. 7 refs. (orig.)

  2. Spin fluctuations and the

    Directory of Open Access Journals (Sweden)

    V.M. Loktev

    2008-09-01

    Full Text Available We analyze the spectral properties of a phenomenological model for a weakly doped two-dimensional antiferromagnet, in which the carriers move within one of the two sublattices where they were introduced. Such a constraint results in the free carrier spectra with the maxima at k=(± π/2 , ± π/2 observed in some cuprates. We consider the spectral properties of the model by taking into account fluctuations of the spins in the antiferromagnetic background. We show that such fluctuations lead to a non-pole-like structure of the single-hole Green's function and these fluctuations can be responsible for some anomalous "strange metal" properties of underdoped cuprates in the nonsuperconducting regime.

  3. Studying the relationship between redox and cell growth using quantitative phase imaging (Conference Presentation)

    Science.gov (United States)

    Sridharan, Shamira; Leslie, Matthew T.; Bapst, Natalya; Smith, John; Gaskins, H. Rex; Popescu, Gabriel

    2016-03-01

    Quantitative phase imaging has been used in the past to study the dry mass of cells and study cell growth under various treatment conditions. However, the relationship between cellular redox and growth rates has not yet been studied in this context. This study employed the recombinant Glrx-roGFP2 redox biosensor targeted to the mitochondrial matrix or cytosolic compartments of A549 lung epithelial carcinoma cells. The Glrx-roGFP2s biosensor consists of a modified GFP protein containing internal cysteine residues sensitive to the local redox environment. The formation/dissolution of sulfide bridges contorts the internal chromophore, dictating corresponding changes in florescence emission that provide direct measures of the local redox potential. Combining 2-channel florescent imaging of the redox sensor with quantitative phase imaging allowed observation of redox homeostasis alongside measurements of cellular mass during full cycles of cellular division. The results indicate that mitochondrial redox showed a stronger inverse correlation with cell growth than cytoplasmic redox states; although redox changes are restricted to a 5% range. We are now studying the relationship between mitochondrial redox and cell growth in an isogenic series of breast cell lines built upon the MCF-10A genetic background that vary both in malignancy and metastatic potential.

  4. Longitudinal fluctuations and decorrelation of anisotropic flow

    Energy Technology Data Exchange (ETDEWEB)

    Pang, Long-Gang [Frankfurt Institute for Advanced Studies, Ruth-Moufang-Strasse 1, 60438 Frankfurt am Main (Germany); Petersen, Hannah [Frankfurt Institute for Advanced Studies, Ruth-Moufang-Strasse 1, 60438 Frankfurt am Main (Germany); Institute for Theoretical Physics, Goethe University, Max-von-Laue-Strasse 1, 60438 Frankfurt am Main (Germany); GSI Helmholtzzentrum für Schwerionenforschung, Planckstr. 1, 64291 Darmstadt (Germany); Qin, Guang-You [Key Laboratory of Quark & Lepton Physics (MOE) and Institute of Particle Physics, Central China Normal University, Wuhan 430079 (China); Roy, Victor [Institute for Theoretical Physics, Goethe University, Max-von-Laue-Strasse 1, 60438 Frankfurt am Main (Germany); Wang, Xin-Nian [Key Laboratory of Quark & Lepton Physics (MOE) and Institute of Particle Physics, Central China Normal University, Wuhan 430079 (China); Nuclear Science Division MS70R0319, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)

    2016-12-15

    We investigate the decorrelation of 2nd and 3rd order anisotropic flow for charged particles in two different pseudo rapidity (η) windows by varying the pseudo rapidity gap, in an event-by-event (3+1)D ideal hydrodynamic model, with fluctuating initial conditions from A Multi-Phase Transport (AMPT) model. We visualize the parton distribution at initial state for Pb+Pb collisions at LHC and Au+Au collisions at RHIC, and demonstrate the longitudinal fluctuations originating from the asymmetry between forward and backward going participants, the fluctuations of the string length and the fluctuations due to finite number of partons at different beam energies. The decorrelation of anisotropic flow of final hadrons with large η gaps is found to originate from the spatial decorrelation along the longitudinal direction in the AMPT initial conditions through hydrodynamic evolution. The agreement between our results and recent CMS data in most centralities suggests that the string-like mechanism of initial parton production in AMPT model captures the initial longitudinal fluctuation that is responsible for the measured decorrelation of anisotropic flow in Pb+Pb collisions at LHC. Our predictions for Au+Au collisions at the highest RHIC energy show stronger longitudinal decorrelation than at LHC, indicating larger longitudinal fluctuations at lower beam energies.

  5. The fluctuating gap model

    International Nuclear Information System (INIS)

    Cao, Xiaobin

    2011-01-01

    The quasi-one-dimensional systems exhibit some unusual phenomenon, such as the Peierls instability, the pseudogap phenomena and the absence of a Fermi-Dirac distribution function line shape in the photoemission spectroscopy. Ever since the discovery of materials with highly anisotropic properties, it has been recognized that fluctuations play an important role above the three-dimensional phase transition. This regime where the precursor fluctuations are presented can be described by the so called fluctuating gap model (FGM) which was derived from the Froehlich Hamiltonian to study the low energy physics of the one-dimensional electron-phonon system. Not only is the FGM of great interest in the context of quasi-one-dimensional materials, liquid metal and spin waves above T c in ferromagnets, but also in the semiclassical approximation of superconductivity, it is possible to replace the original three-dimensional problem by a directional average over effectively one-dimensional problem which in the weak coupling limit is described by the FGM. In this work, we investigate the FGM in a wide temperature range with different statistics of the order parameter fluctuations. We derive a formally exact solution to this problem and calculate the density of states, the spectral function and the optical conductivity. In our calculation, we show that a Dyson singularity appears in the low energy density of states for Gaussian fluctuations in the commensurate case. In the incommensurate case, there is no such kind of singularity, and the zero frequency density of states varies differently as a function of the correlation lengths for different statistics of the order parameter fluctuations. Using the density of states we calculated with non-Gaussian order parameter fluctuations, we are able to calculate the static spin susceptibility which agrees with the experimental data very well. In the calculation of the spectral functions, we show that as the correlation increases, the quasi

  6. The fluctuating gap model

    Energy Technology Data Exchange (ETDEWEB)

    Cao, Xiaobin

    2011-01-15

    The quasi-one-dimensional systems exhibit some unusual phenomenon, such as the Peierls instability, the pseudogap phenomena and the absence of a Fermi-Dirac distribution function line shape in the photoemission spectroscopy. Ever since the discovery of materials with highly anisotropic properties, it has been recognized that fluctuations play an important role above the three-dimensional phase transition. This regime where the precursor fluctuations are presented can be described by the so called fluctuating gap model (FGM) which was derived from the Froehlich Hamiltonian to study the low energy physics of the one-dimensional electron-phonon system. Not only is the FGM of great interest in the context of quasi-one-dimensional materials, liquid metal and spin waves above T{sub c} in ferromagnets, but also in the semiclassical approximation of superconductivity, it is possible to replace the original three-dimensional problem by a directional average over effectively one-dimensional problem which in the weak coupling limit is described by the FGM. In this work, we investigate the FGM in a wide temperature range with different statistics of the order parameter fluctuations. We derive a formally exact solution to this problem and calculate the density of states, the spectral function and the optical conductivity. In our calculation, we show that a Dyson singularity appears in the low energy density of states for Gaussian fluctuations in the commensurate case. In the incommensurate case, there is no such kind of singularity, and the zero frequency density of states varies differently as a function of the correlation lengths for different statistics of the order parameter fluctuations. Using the density of states we calculated with non-Gaussian order parameter fluctuations, we are able to calculate the static spin susceptibility which agrees with the experimental data very well. In the calculation of the spectral functions, we show that as the correlation increases, the

  7. A Membrane-Free Redox Flow Battery with Two Immiscible Redox Electrolytes.

    Science.gov (United States)

    Navalpotro, Paula; Palma, Jesus; Anderson, Marc; Marcilla, Rebeca

    2017-10-02

    Flexible and scalable energy storage solutions are necessary for mitigating fluctuations of renewable energy sources. The main advantage of redox flow batteries is their ability to decouple power and energy. However, they present some limitations including poor performance, short-lifetimes, and expensive ion-selective membranes as well as high price, toxicity, and scarcity of vanadium compounds. We report a membrane-free battery that relies on the immiscibility of redox electrolytes and where vanadium is replaced by organic molecules. We show that the biphasic system formed by one acidic solution and one ionic liquid, both containing quinoyl species, behaves as a reversible battery without any membrane. This proof-of-concept of a membrane-free battery has an open circuit voltage of 1.4 V with a high theoretical energy density of 22.5 Wh L -1 , and is able to deliver 90 % of its theoretical capacity while showing excellent long-term performance (coulombic efficiency of 100 % and energy efficiency of 70 %). © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

  8. Characterisation of the Redox Sensitive NMDA Receptor

    KAUST Repository

    Alzahrani, Ohood

    2016-05-01

    Glucose entry into the brain and its subsequent metabolism to L-lactate, regulated by astrocytes, plays a major role in synaptic plasticity and memory formation. A recent study has shown that L-lactate produced by the brain upon stimulation of glycolysis, and glycogen-derived L-lactate from astrocytes and its transport into neurons, is crucial for memory formation. A recent study revealed the molecular mechanisms that underlie the role of L-lactate in neuronal plasticity and long-term memory formation. L-lactate was shown to induce a cascade of molecular events via modulation of redox-sensitive N-Methyl-D-aspartate (NMDA) receptor activity that was mimicked by nicotinamide adenine dinucleotide hydride (NADH) co-enzyme. This indicated that changes in cellular redox state, following L-lactate transport inside the cells and its subsequent metabolism, production of NADH, and favouring a reduced state are the key effects of L-lactate. Therefore, we are investigating the role of L-lactate in modulating NMDA receptor function via redox modulatory sites. Accordingly, crucial redox-sensitive cysteine residues, Cys320 and Cys87, of the NR2A NMDA receptor subunit are mutated using site-directed mutation, transfected, and expressed in HEK293 cells. This cellular system will then be used to characterise and monitor its activity upon Llactate stimulation, compared to the wild type. This will be achieved by calcium imaging, using fluorescent microscopy. Our data shows that L-lactate potentiated NMDA receptor activity and increased intracellular calcium influx in NR1/NR2A wild type compared to the control condition (WT NR1/NR2A perfused with (1μM) glutamate and (1μM) glycine agonist only), showing faster response initiation and slower decay rate of the calcium signal to the baseline. Additionally, stimulating with L-lactate associated with greater numbers of cells having high fluorescent intensity (peak amplitude) compared to the control. Furthermore, L-lactate rescued the

  9. Terrestrial Gravity Fluctuations

    Directory of Open Access Journals (Sweden)

    Jan Harms

    2015-12-01

    Full Text Available Different forms of fluctuations of the terrestrial gravity field are observed by gravity experiments. For example, atmospheric pressure fluctuations generate a gravity-noise foreground in measurements with super-conducting gravimeters. Gravity changes caused by high-magnitude earthquakes have been detected with the satellite gravity experiment GRACE, and we expect high-frequency terrestrial gravity fluctuations produced by ambient seismic fields to limit the sensitivity of ground-based gravitational-wave (GW detectors. Accordingly, terrestrial gravity fluctuations are considered noise and signal depending on the experiment. Here, we will focus on ground-based gravimetry. This field is rapidly progressing through the development of GW detectors. The technology is pushed to its current limits in the advanced generation of the LIGO and Virgo detectors, targeting gravity strain sensitivities better than 10^–23 Hz^–1/2 above a few tens of a Hz. Alternative designs for GW detectors evolving from traditional gravity gradiometers such as torsion bars, atom interferometers, and superconducting gradiometers are currently being developed to extend the detection band to frequencies below 1 Hz. The goal of this article is to provide the analytical framework to describe terrestrial gravity perturbations in these experiments. Models of terrestrial gravity perturbations related to seismic fields, atmospheric disturbances, and vibrating, rotating or moving objects, are derived and analyzed. The models are then used to evaluate passive and active gravity noise mitigation strategies in GW detectors, or alternatively, to describe their potential use in geophysics. The article reviews the current state of the field, and also presents new analyses especially with respect to the impact of seismic scattering on gravity perturbations, active gravity noise cancellation, and time-domain models of gravity perturbations from atmospheric and seismic point sources. Our

  10. Terrestrial Gravity Fluctuations

    Science.gov (United States)

    Harms, Jan

    2015-12-01

    Different forms of fluctuations of the terrestrial gravity field are observed by gravity experiments. For example, atmospheric pressure fluctuations generate a gravity-noise foreground in measurements with super-conducting gravimeters. Gravity changes caused by high-magnitude earthquakes have been detected with the satellite gravity experiment GRACE, and we expect high-frequency terrestrial gravity fluctuations produced by ambient seismic fields to limit the sensitivity of ground-based gravitational-wave (GW) detectors. Accordingly, terrestrial gravity fluctuations are considered noise and signal depending on the experiment. Here, we will focus on ground-based gravimetry. This field is rapidly progressing through the development of GW detectors. The technology is pushed to its current limits in the advanced generation of the LIGO and Virgo detectors, targeting gravity strain sensitivities better than 10-23 Hz-1/2 above a few tens of a Hz. Alternative designs for GW detectors evolving from traditional gravity gradiometers such as torsion bars, atom interferometers, and superconducting gradiometers are currently being developed to extend the detection band to frequencies below 1 Hz. The goal of this article is to provide the analytical framework to describe terrestrial gravity perturbations in these experiments. Models of terrestrial gravity perturbations related to seismic fields, atmospheric disturbances, and vibrating, rotating or moving objects, are derived and analyzed. The models are then used to evaluate passive and active gravity noise mitigation strategies in GW detectors, or alternatively, to describe their potential use in geophysics. The article reviews the current state of the field, and also presents new analyses especially with respect to the impact of seismic scattering on gravity perturbations, active gravity noise cancellation, and time-domain models of gravity perturbations from atmospheric and seismic point sources. Our understanding of

  11. Synthesis and characterization of redox-active ferric nontronite

    Energy Technology Data Exchange (ETDEWEB)

    Ilgen, A. G.; Kukkadapu, R. K.; Dunphy, D. R.; Artyushkova, K.; Cerrato, J. M.; Kruichak, J. N.; Janish, M. T.; Sun, C. J.; Argo, J. M.; Washington, R. E.

    2017-10-01

    Heterogeneous redox reactions on clay mineral surfaces control mobility and bioavailability of redox-sensitive nutrients and contaminants. Iron (Fe) residing in clay mineral structures can either catalyze or directly participate in redox reactions; however, chemical controls over its reactivity are not fully understood. In our previous work we demonstrated that converting a minor portion of Fe(III) to Fe(II) (partial reduction) in the octahedral sheet of natural Fe-rich clay mineral nontronite (NAu-1) activates its surface, making it redox-active. In this study we produced and characterized synthetic ferric nontronite (SIP), highlighting structural and chemical similarities and differences between this synthetic nontronite and its natural counterpart NAu-1, and probed whether mineral surface is redox-active by reacting it with arsenic As(III) under oxic and anoxic conditions. We demonstrate that synthetic nontronite SIP undergoes the same activation as natural nontronite NAu-1 following the partial reduction treatment. Similar to NAu-1, SIP oxidized As(III) to As(V) under both oxic (catalytic pathway) and anoxic (direct oxidation) conditions. The similar reactivity trends observed for synthetic nontronite and its natural counterpart make SIP an appropriate analog for laboratory studies. The development of chemically pure analogs for ubiquitous soil minerals will allow for systematic research of the fundamental properties of these minerals.

  12. conditions

    Directory of Open Access Journals (Sweden)

    M. Venkatesulu

    1996-01-01

    Full Text Available Solutions of initial value problems associated with a pair of ordinary differential systems (L1,L2 defined on two adjacent intervals I1 and I2 and satisfying certain interface-spatial conditions at the common end (interface point are studied.

  13. Redox reactions in food fermentations

    DEFF Research Database (Denmark)

    Hansen, Egon Bech

    2018-01-01

    Food fermentations are typically performed without actively supplying air. Except for possible surface microorganisms, oxygen will only be transiently available and the redox reactions during the fermentation need to be in balance. Production of ATP from fermentation of carbohydrates typically in...... of the redox properties of strains used to compose food cultures.......Food fermentations are typically performed without actively supplying air. Except for possible surface microorganisms, oxygen will only be transiently available and the redox reactions during the fermentation need to be in balance. Production of ATP from fermentation of carbohydrates typically...... involves oxidative steps in the early part of the pathways whereas a multitude of different reactions are used as compensating reductions. Much of the diversity seen between food fermentations arise from the different routes and the different electron acceptors used by microorganisms to counterbalance...

  14. Redox Pioneer: Professor Joseph Loscalzo

    OpenAIRE

    Leopold, Jane A.

    2010-01-01

    Dr. Joseph Loscalzo (M.D., 1978; Ph.D., 1977) is recognized here as a Redox Pioneer because he has published two articles in the field of antioxidant/redox biology that have been cited more than 1,000 times and 22 articles that have been cited more than 100 times. Dr. Loscalzo is known for his seminal contributions to our understanding of the vascular biology of nitric oxide. His initial discovery that the antiplatelet effects of organic nitrates are potentiated by thiols through a mechanism ...

  15. Fluctuations in quantum devices

    Directory of Open Access Journals (Sweden)

    H.Haken

    2004-01-01

    Full Text Available Logical gates can be formalized by Boolean algebra whose elementary operations can be realized by devices that employ the interactions of macroscopic numbers of elementary excitations such as electrons, holes, photons etc. With increasing miniaturization to the nano scale and below, quantum fluctuations become important and can no longer be ignored. Based on Heisenberg equations of motion for the creation and annihilation operators of elementary excitations, I determine the noise sources of composite quantum systems.

  16. Physics of fashion fluctuations

    Science.gov (United States)

    Donangelo, R.; Hansen, A.; Sneppen, K.; Souza, S. R.

    2000-12-01

    We consider a market where many agents trade different types of products with each other. We model development of collective modes in this market, and quantify these by fluctuations that scale with time with a Hurst exponent of about 0.7. We demonstrate that individual products in the model occasionally become globally accepted means of exchange, and simultaneously become very actively traded. Thus collective features similar to money spontaneously emerge, without any a priori reason.

  17. Quantitative proteomic characterization of redox-dependent post-translational modifications on protein cysteines

    Energy Technology Data Exchange (ETDEWEB)

    Duan, Jicheng; Gaffrey, Matthew J.; Qian, Wei-Jun

    2017-01-01

    Protein cysteine thiols play a crucial role in redox signaling, regulation of enzymatic activity and protein function, and maintaining redox homeostasis in living systems. The unique chemical reactivity of thiol groups makes cysteine susceptible to oxidative modifications by reactive oxygen and nitrogen species to form a broad array of reversible and irreversible protein post-translational modifications (PTMs). The reversible modifications in particular are one of the major components of redox signaling and are involved in regulation of various cellular processes under physiological and pathological conditions. The biological significance of these redox PTMs in health and diseases has been increasingly recognized. Herein, we review the recent advances of quantitative proteomic approaches for investigating redox PTMs in complex biological systems, including the general considerations of sample processing, various chemical or affinity enrichment strategies, and quantitative approaches. We also highlight a number of redox proteomic approaches that enable effective profiling of redox PTMs for addressing specific biological questions. Although some technological limitations remain, redox proteomics is paving the way towards a better understanding of redox signaling and regulation in human health and diseases.

  18. Sedimentary cobalt concentrations track marine redox evolution

    Science.gov (United States)

    Swanner, Elizabeth; Planavsky, Noah; Lalonde, Stefan; Robbins, Jamie; Bekker, Andrey; Rouxel, Olivier; Konhauser, Kurt O.; Mojzsis, Stephen J.

    2013-04-01

    Oxygen production by photosynthesis drove the redox evolution of the atmosphere and ocean. Primary productivity by oxygenic photosynthesizers in the modern surface ocean is limited by trace nutrients such as iron, but previous studies have also observed high Co uptake associated with natural cyanobacterial populations. Constraining the size and variation of the oceanic reservoir of Co through time will help to understand the regulation of primary productivity and hence oxygenation through time. In this study, Co concentrations from iron formations (IF), shales and marine pyrites deposited over nearly 4 billion years of Earth's history are utilized to reconstruct secular changes in the mechanisms of Co removal from the oceanic reservoir. The Co reservoir prior to ~2 Ga was dominated by hydrothermal inputs and Fe(III)oxyhydroxides were likely involved in the removal of Co from the water column. Fe(II) oxidation in the water column resulted in the deposition of IF in the Archean and Paleoproterozoic, and the Co inventory of IF records a large oceanic reservoir of Co during this time. Lower Co concentrations in sediments during the Middle Proterozoic signify a decrease in the oceanic reservoir due to the expansion euxinic environments, corresponding to the results of previous studies. A transition to an oxidized deep ocean in the Phanerozoic is evidenced by correlation between Co and manganese (Mn) concentrations in hydrothermal and exhalative deposits, and in marine pyrites. This relationship between Co and Mn, signifying deposition of Co in association with Mn(IV)oxides, does not occur in the Precambrian. Mn(II) oxidation occurs at higher redox potentials than that required for Fe(II) oxidation, and the extent of Mn redox cycling prior to full ventilation of the oceans at the end of the Neoproterozoic was likely limited to spatially restricted oxic surface waters. In this regard, Co is another valuable redox proxy for tracking the growth and decline in oxygenated

  19. Redox zones of a landfill leachate pollution plume (Vejen, Denmark)

    DEFF Research Database (Denmark)

    Lyngkilde, John; Christensen, Thomas Højlund

    1992-01-01

    Downgradient from an old municipal landfill allowing leachate, rich in dissolved organic carbon, to enter a shallow sandy aerobic aquifer, a sequence of redoxe zones is identified from groundwater chemical analysis. Below the landfill, methanogenic conditions prevail, followed by sulfidogenic...... the fate of reactive pollutants leached from the landfill....

  20. Testing and improving the redox stability of Ni-based solid oxide fuel cells

    DEFF Research Database (Denmark)

    Pihlatie, Mikko; Ramos, Tania; Kaiser, Andreas

    2009-01-01

    cells. When the symmetric cells are reduced and redox cycled isothermally at 850 °C, no major change in the serial or polarisation resistance of the cell and electrodes was measured. When the cells are, after the similar initial reduction treatment, redox cycled at 650 °C, the serial resistance remains......Despite active development, solid oxide fuel cells (SOFCs) based on Ni-YSZ anodes still suffer from thermomechanical instability under conditions where the anode side is exposed to oxidising conditions at high temperature. In the first part of the paper, structures and solutions, which could...... improve the redox stability of Ni-YSZ anode supported SOFC's in terms of dimensional and mechanical stability are reported. Porosity is identified as a major microstructural parameter linked to the dimensional and structural stability during redox cycling. The cumulative redox strain (CRS) after three...

  1. Molecular Controls of the Oxygenation and Redox Reactions of Hemoglobin

    Science.gov (United States)

    Henkens, Robert; Alayash, Abdu I.; Banerjee, Sambuddha; Crumbliss, Alvin L.

    2013-01-01

    Abstract Significance: The broad classes of O2-binding proteins known as hemoglobins (Hbs) carry out oxygenation and redox functions that allow organisms with significantly different physiological demands to exist in a wide range of environments. This is aided by allosteric controls that modulate the protein's redox reactions as well as its O2-binding functions. Recent Advances: The controls of Hb's redox reactions can differ appreciably from the molecular controls for Hb oxygenation and come into play in elegant mechanisms for dealing with nitrosative stress, in the malarial resistance conferred by sickle cell Hb, and in the as-yet unsuccessful designs for safe and effective blood substitutes. Critical Issues: An important basic principle in consideration of Hb's redox reactions is the distinction between kinetic and thermodynamic reaction control. Clarification of these modes of control is critical to gaining an increased understanding of Hb-mediated oxidative processes and oxidative toxicity in vivo. Future Directions: This review addresses emerging concepts and some unresolved questions regarding the interplay between the oxygenation and oxidation reactions of structurally diverse Hbs, both within red blood cells and under acellular conditions. Developing methods that control Hb-mediated oxidative toxicity will be critical to the future development of Hb-based blood substitutes. Antioxid. Redox Signal. 18, 2298–2313. PMID:23198874

  2. Surface Fluctuation Scattering using Grating Heterodyne Spectroscopy

    DEFF Research Database (Denmark)

    Edwards, R. V.; Sirohi, R. S.; Mann, J. A.

    1982-01-01

    Heterodyne photon spectroscopy is used for the study of the viscoelastic properties of the liquid interface by studying light scattered from thermally generated surface fluctuations. A theory of a heterodyne apparatus based on a grating is presented, and the heterodyne condition is given in terms...

  3. Redox freezing and melting in the Earth's deep mantle resulting from carbon-iron redox coupling.

    Science.gov (United States)

    Rohrbach, Arno; Schmidt, Max W

    2011-04-14

    Very low seismic velocity anomalies in the Earth's mantle may reflect small amounts of melt present in the peridotite matrix, and the onset of melting in the Earth's upper mantle is likely to be triggered by the presence of small amounts of carbonate. Such carbonates stem from subducted oceanic lithosphere in part buried to depths below the 660-kilometre discontinuity and remixed into the mantle. Here we demonstrate that carbonate-induced melting may occur in deeply subducted lithosphere at near-adiabatic temperatures in the Earth's transition zone and lower mantle. We show experimentally that these carbonatite melts are unstable when infiltrating ambient mantle and are reduced to immobile diamond when recycled at depths greater than ∼250 kilometres, where mantle redox conditions are determined by the presence of an (Fe,Ni) metal phase. This 'redox freezing' process leads to diamond-enriched mantle domains in which the Fe(0), resulting from Fe(2+) disproportionation in perovskites and garnet, is consumed but the Fe(3+) preserved. When such carbon-enriched mantle heterogeneities become part of the upwelling mantle, diamond will inevitably react with the Fe(3+) leading to true carbonatite redox melting at ∼660 and ∼250 kilometres depth to form deep-seated melts in the Earth's mantle.

  4. Strain fluctuations and elastic constants

    Energy Technology Data Exchange (ETDEWEB)

    Parrinello, M.; Rahman, A.

    1982-03-01

    It is shown that the elastic strain fluctuations are a direct measure of elastic compliances in a general anisotropic medium; depending on the ensemble in which the fluctuation is measured either the isothermal or the adiabatic compliances are obtained. These fluctuations can now be calculated in a constant enthalpy and pressure, and hence, constant entropy, ensemble due to recent develpments in the molecular dynamics techniques. A calculation for a Ni single crystal under uniform uniaxial 100 tensile or compressive load is presented as an illustration of the relationships derived between various strain fluctuations and the elastic modulii. The Born stability criteria and the behavior of strain fluctuations are shown to be related.

  5. Gambling with Superconducting Fluctuations

    Science.gov (United States)

    Foltyn, Marek; Zgirski, Maciej

    2015-08-01

    Josephson junctions and superconducting nanowires, when biased close to superconducting critical current, can switch to a nonzero voltage state by thermal or quantum fluctuations. The process is understood as an escape of a Brownian particle from a metastable state. Since this effect is fully stochastic, we propose to use it for generating random numbers. We present protocol for obtaining random numbers and test the experimentally harvested data for their fidelity. Our work is prerequisite for using the Josephson junction as a tool for stochastic (probabilistic) determination of physical parameters such as magnetic flux, temperature, and current.

  6. Classical and quantum temperature fluctuations via holography

    Energy Technology Data Exchange (ETDEWEB)

    Balatsky, Alexander V. [KTH Royal Inst. of Technology, Stockholm (Sweden); Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Gudnason, Sven Bjarke [KTH Royal Inst. of Technology, Stockholm (Sweden); Thorlacius, Larus [KTH Royal Inst. of Technology, Stockholm (Sweden); University of Iceland, Reykjavik (Iceland); Zarembo, Konstantin [KTH Royal Inst. of Technology, Stockholm (Sweden); Inst. of Theoretical and Experimental Physics (ITEP), Moscow (Russian Federation); Uppsala Univ. (Sweden); Krikun, Alexander [KTH Royal Inst. of Technology, Stockholm (Sweden); Inst. of Theoretical and Experimental Physics (ITEP), Moscow (Russian Federation); Kedem, Yaron [KTH Royal Inst. of Technology, Stockholm (Sweden)

    2014-05-27

    We study local temperature fluctuations in a 2+1 dimensional CFT on the sphere, dual to a black hole in asymptotically AdS space-time. The fluctuation spectrum is governed by the lowest-lying hydrodynamic sound modes of the system whose frequency and damping rate determine whether temperature fluctuations are thermal or quantum. We calculate numerically the corresponding quasinormal frequencies and match the result with the hydrodynamics of the dual CFT at large temperature. As a by-product of our analysis we determine the appropriate boundary conditions for calculating low-lying quasinormal modes for a four-dimensional Reissner-Nordstrom black hole in global AdS.

  7. Thallium isotopes track fluctuations in global manganese oxide burial during the Ediacaran Period

    Science.gov (United States)

    Ostrander, C. M.; Nielsen, S.; Owens, J. D.; Jiang, G.; Planavsky, N.; Sahoo, S. K.; Zhang, F.; Lyons, T. W.; Anbar, A. D.

    2017-12-01

    Complex marine ecosystems appear in the geologic record for the first time during the Ediacaran (635 - 541 Ma), after the Marinoan Glaciation but before the Cambrian Explosion. Much debate surrounds the redox-state of global oceans during this diversification, with some arguing for pervasive anoxic conditions and others for increased oxygenation, including the possibility of episodic oxygen increases. Here, we use thallium (Tl) isotopes preserved in organic-rich shales from a deep-water section at Wuhe, South China, to track large-scale perturbations in Mn oxide burial during the Ediacaran. Changes to the Tl isotope composition of seawater over geologic timescales are driven dominantly by fluctuations in global Mn oxide burial, which require persistent O2 at the sediment-water interface. Importantly, the suite of sedimentary rocks analyzed is thought to have been deposited beneath persistent localized euxinia, which is an environment shown to effectively capture the Tl isotope composition of seawater. Within samples previously suggested to host oceanic oxygenation episodes (OOEs) because of high redox-sensitive element (RSE) enrichments (Sahoo et al. 2016, Geobiology), we find Tl isotope values as light as -5 epsilon units, which are indicative of removal of heavy Tl by Mn oxides elsewhere in the Ediacaran ocean and in-line with the presence of deep-marine O2. Intriguingly, between these events, during periods previously viewed as dominantly anoxic, we find Tl isotope excursions to values that are even lighter than during the OOEs (less than -10 epsilon units). To first order, these results imply that an even larger Mn oxide sink was present between the OOEs, which would require pervasive oceanic oxygenation. This interpretation is in direct conflict with interpretations of low RSE enrichments in these same samples, which invoke reservoir drawdown due to widespread anoxia—as well as many other data that suggest dominantly anoxic deep marine conditions through the

  8. Impact of Redox on Glass Durability: The Glass Selection Process

    International Nuclear Information System (INIS)

    PEELER, DAVID

    2004-01-01

    objective s: (1) the impact of REDOX on glass durability (as measured by the Product Consistency Test (PCT)) and (2) the interactive effects that may mitigate the predicted negative impacts based on current free energy of hydration model theory. These glasses will be batched and melted under conditions that target both the projected compositions and intended REDOX states. Durability (as measured by the PCT) will be experimentally determined for each glass in triplicate using standard procedures. The measured response will then be compared to model based predictions to assess the applicability and/or potential conservatism of the model under REDOX activated conditions

  9. NAD(H) and NADP(H) Redox Couples and Cellular Energy Metabolism.

    Science.gov (United States)

    Xiao, Wusheng; Wang, Rui-Sheng; Handy, Diane E; Loscalzo, Joseph

    2018-01-20

    The nicotinamide adenine dinucleotide (NAD + )/reduced NAD + (NADH) and NADP + /reduced NADP + (NADPH) redox couples are essential for maintaining cellular redox homeostasis and for modulating numerous biological events, including cellular metabolism. Deficiency or imbalance of these two redox couples has been associated with many pathological disorders. Recent Advances: Newly identified biosynthetic enzymes and newly developed genetically encoded biosensors enable us to understand better how cells maintain compartmentalized NAD(H) and NADP(H) pools. The concept of redox stress (oxidative and reductive stress) reflected by changes in NAD(H)/NADP(H) has increasingly gained attention. The emerging roles of NAD + -consuming proteins in regulating cellular redox and metabolic homeostasis are active research topics. The biosynthesis and distribution of cellular NAD(H) and NADP(H) are highly compartmentalized. It is critical to understand how cells maintain the steady levels of these redox couple pools to ensure their normal functions and simultaneously avoid inducing redox stress. In addition, it is essential to understand how NAD(H)- and NADP(H)-utilizing enzymes interact with other signaling pathways, such as those regulated by hypoxia-inducible factor, to maintain cellular redox homeostasis and energy metabolism. Additional studies are needed to investigate the inter-relationships among compartmentalized NAD(H)/NADP(H) pools and how these two dinucleotide redox couples collaboratively regulate cellular redox states and cellular metabolism under normal and pathological conditions. Furthermore, recent studies suggest the utility of using pharmacological interventions or nutrient-based bioactive NAD + precursors as therapeutic interventions for metabolic diseases. Thus, a better understanding of the cellular functions of NAD(H) and NADP(H) may facilitate efforts to address a host of pathological disorders effectively. Antioxid. Redox Signal. 28, 251-272.

  10. Mesoscale wind fluctuations over Danish waters

    Energy Technology Data Exchange (ETDEWEB)

    Vincent, C.L.

    2010-12-15

    Mesoscale wind fluctuations affect the large scale integration of wind power because they undermine the day-ahead predictability of wind speed and power production, and because they can result in large fluctuations in power generation that must be balanced using reserve power. Large fluctuations in generated power are a particular problem for offshore wind farms because the typically high concentration of turbines within a limited geographical area means that fluctuations can be correlated across large numbers of turbines. Furthermore, organised mesoscale structures that often form over water, such as convective rolls and cellular convection, have length scales of tens of kilometers, and can cause large wind fluctuations on a time scale of around an hour. This thesis is an exploration of the predictability of mesoscale wind fluctuations using observations from the world's first two large offshore wind farms - Horns Rev I in the North Sea, and Nysted in the Baltic Sea. The thesis begins with a climatological analysis of wind fluctuations on time scales of 1-10 hours at the two sites. A novel method for calculating conditional climatologies of spectral information is proposed, based on binning and averaging the time axis of the Hilbert spectrum. Results reveal clear patterns between wind fluctuations and locally observed meteorological conditions. The analysis is expanded by classifying wind fluctuations on time scales of 1-3 hours according to synoptic patterns, satellite pictures and wind classes. Results indicate that cold air outbreaks and open cellular convection are a significant contributor to mesoscale wind variability at Horns Rev. The predictability of mesoscale wind fluctuations is tested by implementing standard statistical models that relate local wind variability to parameters based on a large scale weather analysis. The models show some skill, but only achieve a 15% improvement on a persistence forecast. The possibility of explicitly modelling

  11. Acupuncture Mechanism and Redox Equilibrium

    Directory of Open Access Journals (Sweden)

    Xiang-Hong Zeng

    2014-01-01

    Full Text Available Oxidative stress participates in the pathological process of various diseases. Acupuncture is a component of the health care system in China that can be traced back for at least 3000 years. Recently, increased evidences indicate that acupuncture stimulation could reduce oxidative damage in organisms under pathological state, but the exact mechanism remains unclear. This review focuses on the emerging links between acupuncture and redox modulation in various disorders, such as vascular dementia, Parkinson’s disease, and hypertension, ranging from redox system, antioxidant system, anti-inflammatory system, and nervous system to signaling pathway. Although the molecular and cellular pathways studies of acupuncture effect on oxidative stress are preliminary, they represent an important step forward in the research of acupuncture antioxidative effect.

  12. Benzene dynamics and biodegradation in alluvial aquifers affected by river fluctuations.

    Science.gov (United States)

    Batlle-Aguilar, J; Morasch, B; Hunkeler, D; Brouyère, S

    2014-01-01

    The spatial distribution and temporal dynamics of a benzene plume in an alluvial aquifer strongly affected by river fluctuations was studied. Benzene concentrations, aquifer geochemistry datasets, past river morphology, and benzene degradation rates estimated in situ using stable carbon isotope enrichment were analyzed in concert with aquifer heterogeneity and river fluctuations. Geochemistry data demonstrated that benzene biodegradation was on-going under sulfate reducing conditions. Long-term monitoring of hydraulic heads and characterization of the alluvial aquifer formed the basis of a detailed modeled image of aquifer heterogeneity. Hydraulic conductivity was found to strongly correlate with benzene degradation, indicating that low hydraulic conductivity areas are capable of sustaining benzene anaerobic biodegradation provided the electron acceptor (SO4 (2-) ) does not become rate limiting. Modeling results demonstrated that the groundwater flux direction is reversed on annual basis when the river level rises up to 2 m, thereby forcing the infiltration of oxygenated surface water into the aquifer. The mobilization state of metal trace elements such as Zn, Cd, and As present in the aquifer predominantly depended on the strong potential gradient within the plume. However, infiltration of oxygenated water was found to trigger a change from strongly reducing to oxic conditions near the river, causing mobilization of previously immobile metal species and vice versa. MNA appears to be an appropriate remediation strategy in this type of dynamic environment provided that aquifer characterization and targeted monitoring of redox conditions are adequate and electron acceptors remain available until concentrations of toxic compounds reduce to acceptable levels. © 2013, National Ground Water Association.

  13. Vanadium and Chromium Redox Behavior in borosilicate Nuclear Waste Glasses

    International Nuclear Information System (INIS)

    McKeown, D.; Muller, I.; Gan, H.; Feng, Z.; Viragh, C.; Pegg, I.

    2011-01-01

    X-ray absorption spectroscopy (XAS) was used to characterize vanadium (V) and chromium (Cr) environments in low activity nuclear waste (LAW) glasses synthesized under a variety of redox conditions. V 2 O 5 was added to the melt to improve sulfur incorporation from the waste; however, at sufficiently high concentrations, V increased melt foaming, which lowered melt processing rates. Foaming may be reduced by varying the redox conditions of the melt, while small amounts of Cr are added to reduce melter refractory corrosion. Three parent glasses were studied, where CO-CO 2 mixtures were bubbled through the corresponding melt for increasing time intervals so that a series of redox-adjusted-glasses was synthesized from each parent glass. XAS data indicated that V and Cr behaviors are significantly different in these glasses with respect to the cumulative gas bubbling times: V 4+ /V total ranges from 8 to 35%, while Cr 3+ /Cr total can range from 15 to 100% and even to population distributions including Cr 2+ . As Na-content decreased, V, and especially, Cr became more reduced, when comparing equivalent glasses within a series. The Na-poor glass series show possible redox coupling between V and Cr, where V 4+ populations increase after initial bubbling, but as bubbling time increases, V 4+ populations drop to near the level of the parent glass, while Cr becomes more reduced to the point of having increasing Cr 2+ populations.

  14. Redox signaling in acute pancreatitis

    Science.gov (United States)

    Pérez, Salvador; Pereda, Javier; Sabater, Luis; Sastre, Juan

    2015-01-01

    Acute pancreatitis is an inflammatory process of the pancreatic gland that eventually may lead to a severe systemic inflammatory response. A key event in pancreatic damage is the intracellular activation of NF-κB and zymogens, involving also calcium, cathepsins, pH disorders, autophagy, and cell death, particularly necrosis. This review focuses on the new role of redox signaling in acute pancreatitis. Oxidative stress and redox status are involved in the onset of acute pancreatitis and also in the development of the systemic inflammatory response, being glutathione depletion, xanthine oxidase activation, and thiol oxidation in proteins critical features of the disease in the pancreas. On the other hand, the release of extracellular hemoglobin into the circulation from the ascitic fluid in severe necrotizing pancreatitis enhances lipid peroxidation in plasma and the inflammatory infiltrate into the lung and up-regulates the HIF–VEGF pathway, contributing to the systemic inflammatory response. Therefore, redox signaling and oxidative stress contribute to the local and systemic inflammatory response during acute pancreatitis. PMID:25778551

  15. Fluctuations in email size

    Science.gov (United States)

    Matsubara, Yoshitsugu; Musashi, Yasuo

    2017-12-01

    The purpose of this study is to explain fluctuations in email size. We have previously investigated the long-term correlations between email send requests and data flow in the system log of the primary staff email server at a university campus, finding that email size frequency follows a power-law distribution with two inflection points, and that the power-law property weakens the correlation of the data flow. However, the mechanism underlying this fluctuation is not completely understood. We collected new log data from both staff and students over six academic years and analyzed the frequency distribution thereof, focusing on the type of content contained in the emails. Furthermore, we obtained permission to collect "Content-Type" log data from the email headers. We therefore collected the staff log data from May 1, 2015 to July 31, 2015, creating two subdistributions. In this paper, we propose a model to explain these subdistributions, which follow log-normal-like distributions. In the log-normal-like model, email senders -consciously or unconsciously- regulate the size of new email sentences according to a normal distribution. The fitting of the model is acceptable for these subdistributions, and the model demonstrates power-law properties for large email sizes. An analysis of the length of new email sentences would be required for further discussion of our model; however, to protect user privacy at the participating organization, we left this analysis for future work. This study provides new knowledge on the properties of email sizes, and our model is expected to contribute to the decision on whether to establish upper size limits in the design of email services.

  16. Dissecting Redox Biology Using Fluorescent Protein Sensors.

    Science.gov (United States)

    Schwarzländer, Markus; Dick, Tobias P; Meyer, Andreas J; Morgan, Bruce

    2016-05-01

    Fluorescent protein sensors have revitalized the field of redox biology by revolutionizing the study of redox processes in living cells and organisms. Within one decade, a set of fundamental new insights has been gained, driven by the rapid technical development of in vivo redox sensing. Redox-sensitive yellow and green fluorescent protein variants (rxYFP and roGFPs) have been the central players. Although widely used as an established standard tool, important questions remain surrounding their meaningful use in vivo. We review the growing range of thiol redox sensor variants and their application in different cells, tissues, and organisms. We highlight five key findings where in vivo sensing has been instrumental in changing our understanding of redox biology, critically assess the interpretation of in vivo redox data, and discuss technical and biological limitations of current redox sensors and sensing approaches. We explore how novel sensor variants may further add to the current momentum toward a novel mechanistic and integrated understanding of redox biology in vivo. Antioxid. Redox Signal. 24, 680-712.

  17. Big Bang or vacuum fluctuation

    International Nuclear Information System (INIS)

    Zel'dovich, Ya.B.

    1980-01-01

    Some general properties of vacuum fluctuations in quantum field theory are described. The connection between the ''energy dominance'' of the energy density of vacuum fluctuations in curved space-time and the presence of singularity is discussed. It is pointed out that a de-Sitter space-time (with the energy density of the vacuum fluctuations in the Einstein equations) that matches the expanding Friedman solution may describe the history of the Universe before the Big Bang. (P.L.)

  18. Fitness effects of fluctuations in biochemical networks

    Science.gov (United States)

    Tanase-Nicola, Sorin

    2009-03-01

    The concentration of many cellular components fluctuates not only as a response to external and internal inputs but also due to random birth and death events of individual molecules. This biochemical noise affects the capacity of every individual cell in a population to respond and adapt to the environment. While the sources and effects of biochemical fluctuations on individual cells have been intensively studied, the effects of noise on the growth rate of a population of cells are much less understood. We present a model of the cell cycle in which the growth and division of individual cells are coupled with the noisy dynamics of their internal components. The model allows us to compute the contribution of the biochemical noise to the average growth rate of a population of cells as a function of the noise strength and the correlation time of the fluctuations. We show that, due to fluctuations, the growth rate of a population of cells is always larger than the average growth rate of a individual cell and can be larger even than a corresponding deterministic model. In most relevant cases it is assumed that the average concentration of a cellular component is close to a value that maximizes the population growth as given by the external, environmental, conditions and the internal cellular regulation. In such cases we show that contribution of fluctuations to the growth rate is negative and increases with the sensitivity of the biochemical networks to the noise sources and the noise correlation time. We also discuss how the selection pressure due to fluctuations affects the structure and parameters of genetic regulatory networks.

  19. Molecular analysis of Ku redox regulation

    Directory of Open Access Journals (Sweden)

    Shatilla Andrea

    2009-08-01

    Full Text Available Abstract Background DNA double-strand breaks (DSBs can occur in response to ionizing radiation (IR, radiomimetic agents and from endogenous DNA-damaging reactive oxygen metabolites. Unrepaired or improperly repaired DSBs are potentially the most lethal form of DNA damage and can result in chromosomal translocations and contribute to the development of cancer. The principal mechanism for the repair of DSBs in humans is non-homologous end-joining (NHEJ. Ku is a key member of the NHEJ pathway and plays an important role in the recognition step when it binds to free DNA termini. Ku then stimulates the assembly and activation of other NHEJ components. DNA binding of Ku is regulated by redox conditions and evidence from our laboratory has demonstrated that Ku undergoes structural changes when oxidized that results in a reduction in DNA binding activity. The C-terminal domain and cysteine 493 of Ku80 were investigated for their contribution to redox regulation of Ku. Results We effectively removed the C-terminal domain of Ku80 generating a truncation mutant and co-expressed this variant with wild type Ku70 in an insect cell system to create a Ku70/80ΔC heterodimer. We also generated two single amino acid variants of Cys493, replacing this amino acid with either an alanine (C493A or a serine (C493S, and over-expressed the variant proteins in SF9 insect cells in complex with wild type Ku70. Neither the truncation nor the amino acid substitutions alters protein expression or stability as determined by SDS-PAGE and Western blot analysis. We show that the C493 mutations do not alter the ability of Ku to bind duplex DNA in vitro under reduced conditions while truncation of the Ku80 C-terminus slightly reduced DNA binding affinity. Diamide oxidation of cysteines was shown to inhibit DNA binding similarly for both the wild-type and all variant proteins. Interestingly, differential DNA binding activity following re-reduction was observed for the Ku70/80

  20. Broad temperature adaptability of vanadium redox flow battery—Part 1: Electrolyte research

    International Nuclear Information System (INIS)

    Xiao, Shuibo; Yu, Lihong; Wu, Lantao; Liu, Le; Qiu, Xinping; Xi, Jingyu

    2016-01-01

    Highlights: • Impact of temperature (-35 °C-50 °C) on properties of VRFB electrolyte is studied. • V 2+ , V 3+ , V 3.5+ , V 4+ (VO 2+ ) and V 5+ (VO 2 + ) electrolytes are tested respectively. • V 2+ , V 3+ and V 3.5+ precipitates at low temperature can redissolve when temperature increases. • V 5+ precipitates at high temperature cannot redissolve when temperature decreases. • Conductivity and viscosity of the electrolytes are greatly affected by temperature. - Abstract: The broad temperature adaptability of vanadium redox flow battery (VRFB) is one of the key issues which affects the large-scale and safety application of VRFB. Typically, five types of vanadium electrolytes, namely V 2+ , V 3+ , V 3.5+ (V 3+ :VO 2+ = 1:1), V 4+ (VO 2+ ) and V 5+ (VO 2 + ), are the most common electrolytes' status existing in VRFB system. In this work, the physicochemical and electrochemical properties of these vanadium electrolytes are studied in detail at a broad temperature range (-35 °C–50 °C). The results show that all types of vanadium electrolytes are stable between -25 °C–30 °C. The temperature fluctuation will largely influence the conductivity and viscosity of the electrolytes. Besides, the electrochemical properties of the positive (VO 2+ ) and negative (V 3+ ) electrolytes are greatly affected by the temperature; and the charge transfer process fluctuates more greatly with the temperature variation than the charge diffusion process does. These results enable us to better and more comprehensively evaluate the performance of the electrolyte changing with the temperature, which will be beneficial for the rational choice of electrolyte for VRFB operation under various conditions.

  1. Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state.

    Science.gov (United States)

    Maharjan, Sunita; Sakai, Yasuyoshi; Hoseki, Jun

    2016-01-01

    Mitochondrial impairment and the resulting generation of reactive oxygen species (ROS) have been associated with aging and its related pathological conditions. Recently, dietary antioxidants have gained significant attention as potential preventive and therapeutic agents against ROS-generated aging and pathological conditions. We previously demonstrated that food-derived antioxidants prevented intracellular oxidative stress under proteasome inhibition conditions, which was attributed to mitochondrial dysfunction and ROS generation, followed by cell death. Here, we further screened dietary antioxidants for their activity as redox modulators by visualization of the redox state using Redoxfluor, a fluorescent protein redox probe. Direct alleviation of ROS by antioxidants, but not induction of antioxidative enzymes, prevented mitochondria-mediated intracellular oxidation. The effective antioxidants scavenged mitochondrial ROS and suppressed cell death. Our study indicates that redox visualization under mitochondria-mediated oxidative stress is useful for screening potential antioxidants to counteract mitochondrial dysfunction, which has been implicated in aging and the pathogenesis of aging-related diseases.

  2. Amplified and in situ detection of redox-active metabolite using a biobased redox capacitor.

    Science.gov (United States)

    Kim, Eunkyoung; Gordonov, Tanya; Bentley, William E; Payne, Gregory F

    2013-02-19

    Redox cycling provides a mechanism to amplify electrochemical signals for analyte detection. Previous studies have shown that diverse mediators/shuttles can engage in redox-cycling reactions with a biobased redox capacitor that is fabricated by grafting redox-active catechols onto a chitosan film. Here, we report that redox cycling with this catechol-chitosan redox capacitor can amplify electrochemical signals for detecting a redox-active bacterial metabolite. Specifically, we studied the redox-active bacterial metabolite pyocyanin that is reported to be a virulence factor and signaling molecule for the opportunistic pathogen P. aeruginosa. We demonstrate that redox cycling can amplify outputs from various electrochemical methods (cyclic voltammetry, chronocoulometry, and differential pulse voltammetry) and can lower the detection limit of pyocyanin to 50 nM. Further, the compatibility of this biobased redox capacitor allows the in situ monitoring of the production of redox-active metabolites (e.g., pyocyanin) during the course of P. aeruginosa cultivation. We anticipate that the amplified output of redox-active virulence factors should permit an earlier detection of life-threatening infections by the opportunistic pathogen P. aeruginosa while the "bio-compatibility" of this measurement approach should facilitate in situ study of the spatiotemporal dynamics of bacterial redox signaling.

  3. Redox reactivity and coordination chemistry of uranium

    International Nuclear Information System (INIS)

    Nocton, G.

    2009-09-01

    The study and the understanding of actinides chemistry have important implications in the nuclear field both for the development of new actinides materials and the retreatment of the nuclear wastes. One of the major issues in that chemistry is that the actinides elements are known to undergo redox reaction and to form assemblies of different size and different topologies. In that context uranium can be a good model of the heavier radioelement because it is much less radioactive. So, this work concerns the synthesis and the study of the spectroscopy and the magnetic properties of several uranium based polymetallic assemblies synthesized by taking advantage of the redox properties and the coordination chemistry of uranium. The hydrolysis reactivity of trivalent uranium has been studied in absence of sterically hindered ligands and led to the synthesis of oxo/hydroxo uranium assemblies with different sizes by changing the starting complex or the reaction conditions. By following the same strategy, the controlled oxidation of trivalent uranium complexes led to an original azido/nitrido uranium complex. The coordination chemistry of the pentavalent uranyl polymer {[UO 2 py 5 ][KI 2 py 3 ]} n has also been studied with different ligand and in different conditions and led to several cation-cation complexes for which the stability is sufficient for studying there dismutation by proton NMR. By changing the ancillary ligands stable monomeric complexes of pentavalent uranyl complexes were also obtained. The magnetic properties of all the complexes, monomers and polymetallic complexes were studied and an antiferromagnetic coupling was observed for the cation-cation pentavalent uranyl dimer [UO 2 (dbm) 2 (K 18 C 6 )] 2 . (author)

  4. Magnetic fluctuations associated with density fluctuations in the tokamak edge

    International Nuclear Information System (INIS)

    Kim, Y.J.; Gentle, K.W.; Ritz, C.P.; Rhodes, T.L.; Bengtson, R.D.

    1989-01-01

    Electrostatic density and potential fluctuations occurring with high amplitude near the edge of a tokamak are correlated with components of the fluctuating magnetic field measured outside the limiter radius. It has been established that this turbulence is associated with fluctuations in current as well as density and potential. The correlation extends for substantial toroidal distances, but only if the probes are displaced approximately along field lines, consistent with the short coherence lengths poloidally but long coherence lengths parallel to the field which are characteristic for this turbulence. Furthermore, the correlation can be found only with density fluctuations measured inside the limiter radius; density fluctuations behind the limiter have no detectable magnetic concomitant for the toroidally spaced probes used here. (author). Letter-to-the-editor. 12 refs, 3 figs

  5. Fluctuations of pT from initial size fluctuations

    International Nuclear Information System (INIS)

    Chojnacki, M.; Broniowski, W.; Obara, L.

    2010-01-01

    We investigate the initial transverse size of the source, which comes directly from the Glauber treatment of the earliest stage of relativistic heavy-ion collisions. After the hydrodynamic evolution stage the fluctuations in the transverse velocity flow at the hadronic freeze-out are transformed into the even-by-event fluctuations of the average transverse momentum. The Glauber phase is simulated by GLISSANDO and followed by a realistic hydrodynamic evolution stage. The statistical hadronization is performed by the THERMINATOR. We describe the pT fluctuations at RHIC, in particular the magnitude of the effect, its centrality dependence, and the weak dependence on the incident energy. The results show that the observed event-by-event p T fluctuations are mainly caused by the initial source size fluctuations. (author)

  6. Characterization of redox proteins using electrochemical methods

    OpenAIRE

    Verhagen, M.

    1995-01-01

    The use of electrochemical techniques in combination with proteins started approximately a decade ago and has since then developed into a powerfull technique for the study of small redox proteins. In addition to the determination of redox potentials, electrochemistry can be used to obtain information about the kinetics of electron transfer between proteins and about the dynamic behaviour of redox cofactors in proteins. This thesis describes the results of a study, initiated to get a ...

  7. Radii of Redox Components from Absolute Redox Potentials Compared with Covalent and Aqueous Ionic Radii

    Czech Academy of Sciences Publication Activity Database

    Heyrovská, Raji

    2010-01-01

    Roč. 22, č. 9 (2010), s. 903-907 ISSN 1040-0397 Institutional support: RVO:68081707 Keywords : Electrochemistry * Absolute redox potentials * Radii of redox components Subject RIV: BO - Biophysics Impact factor: 2.721, year: 2010

  8. Fluid Fuel Fluctuations in the Spherical Tank

    Directory of Open Access Journals (Sweden)

    H. D. Nguyen

    2014-01-01

    Full Text Available Many authors tried to solve a task concerning small fluctuations of the incompressible ideal liquid, which partially fills a stationary tank of any shape. There is a long list of references to this subject. The article presents a task solution on own fluctuations of liquid in spherical capacity, with boundary conditions on a free surface and a surface with a resistance – drain surface. Relevance of problem consists in assessment of influence of intra tank devices (measuring, intaking, damping devices, etc. on the liquid fuel fluctuations. The special attention is paid to finding the own values and frequencies of the equations of disturbed flow fluctuations with dissipation available on the boundary surfaces. In contrast to the previous examples, the lowering speed and the free surface area at undisturbed state are variable.The article also considers a variation formulation of the auxiliary boundary tasks. In solution of variation tasks, the attached Legendre's functions were used as coordinate functions. Further, after substitution of the variation tasks solution in the boundary conditions and the subsequent mathematical operations the characteristic equation was obtained. To obtain solutions of the cubic characteristic equation Cardano formulas were used. The article also considers the task on the own motions of liquid filling a capacity between two concentric spheres and flowing out via the intake in case there is a free surface. Reliability of the obtained numerical results is confirmed by comparison with calculation results of frequencies resulting from solutions of a task on the own fluctuations of liquid in the spherical capacity with the constant depth of liquid. All numerical calculations were performed using the Matlab environment.

  9. Differential alkylation-based redox proteomics – Lessons learnt

    Science.gov (United States)

    Wojdyla, Katarzyna; Rogowska-Wrzesinska, Adelina

    2015-01-01

    Cysteine is one of the most reactive amino acids. This is due to the electronegativity of sulphur atom in the side chain of thiolate group. It results in cysteine being present in several distinct redox forms inside the cell. Amongst these, reversible oxidations, S-nitrosylation and S-sulfenylation are crucial mediators of intracellular redox signalling, with known associations to health and disease. Study of their functionalities has intensified thanks to the development of various analytical strategies, with particular contribution from differential alkylation-based proteomics methods. Presented here is a critical evaluation of differential alkylation-based strategies for the analysis of S-nitrosylation and S-sulfenylation. The aim is to assess the current status and to provide insights for future directions in the dynamically evolving field of redox proteomics. To achieve that we collected 35 original research articles published since 2010 and analysed them considering the following parameters, (i) resolution of modification site, (ii) quantitative information, including correction of modification levels by protein abundance changes and determination of modification site occupancy, (iii) throughput, including the amount of starting material required for analysis. The results of this meta-analysis are the core of this review, complemented by issues related to biological models and sample preparation in redox proteomics, including conditions for free thiol blocking and labelling of target cysteine oxoforms. PMID:26282677

  10. Differential alkylation-based redox proteomics--Lessons learnt.

    Science.gov (United States)

    Wojdyla, Katarzyna; Rogowska-Wrzesinska, Adelina

    2015-12-01

    Cysteine is one of the most reactive amino acids. This is due to the electronegativity of sulphur atom in the side chain of thiolate group. It results in cysteine being present in several distinct redox forms inside the cell. Amongst these, reversible oxidations, S-nitrosylation and S-sulfenylation are crucial mediators of intracellular redox signalling, with known associations to health and disease. Study of their functionalities has intensified thanks to the development of various analytical strategies, with particular contribution from differential alkylation-based proteomics methods. Presented here is a critical evaluation of differential alkylation-based strategies for the analysis of S-nitrosylation and S-sulfenylation. The aim is to assess the current status and to provide insights for future directions in the dynamically evolving field of redox proteomics. To achieve that we collected 35 original research articles published since 2010 and analysed them considering the following parameters, (i) resolution of modification site, (ii) quantitative information, including correction of modification levels by protein abundance changes and determination of modification site occupancy, (iii) throughput, including the amount of starting material required for analysis. The results of this meta-analysis are the core of this review, complemented by issues related to biological models and sample preparation in redox proteomics, including conditions for free thiol blocking and labelling of target cysteine oxoforms. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  11. Biomolecules: Fluctuations and relaxations

    Science.gov (United States)

    Parak, F.; Ostermann, A.; Gassmann, A.; Scherk, C.; Chong, S.-H.; Kidera, A.; Go, N.

    1999-10-01

    The normal-mode refinement of X-ray crystallographic data opened a new possibility to analyze the mean-square displacements in a protein molecule. A comparison of the X-ray structure of myoglobin at several temperatures with Mössbauer data is performed. In the low-temperature regime below 180 K the iron mean-square displacements obtained by Mössbauer spectroscopy are in good agreement with a normal-mode analysis. The X-ray mean-square displacements at the position of the iron, after the motion originated from the external degrees of freedom are subtracted, have practically the same temperature dependence as those from Mössbauer spectroscopy. The difference between the X-ray mean-square displacements and those predicted by normal-mode analysis measures the distribution of molecules into conformational substates. Above 180 K the Mössbauer effect indicates fluctuations between conformational substates. The relaxation from a Fe(III) conformation to a Fe(II) conformation is shown for superoxide dismutase of Propionibacterium shermanii.

  12. Behavior of uranium during the formation of granitic magma by anatexis (I). Influence of redox conditions and the presence of chloride on the solubility of uranium in the hydrothermal solutions

    International Nuclear Information System (INIS)

    Satoru Nakashima; Toshimichi Iiyama, J.

    1983-01-01

    The behavior of uranium is examined experimentally in the course of partial fusion of natural or synthetic granitic rocks. Uranium is definitely soluble in the associated hydrothermal solutions containing chloride under oxidizing conditions, but it is not soluble in the same fluids under reducing conditions [fr

  13. Conditioning of NEC accelerator tubes

    International Nuclear Information System (INIS)

    Yntema, J.L.

    1979-01-01

    The conditioning process of NEC tubes was investigated by recording the fluctuations observed with a residual gas analyzer for a fixed mass and a simultaneous recording of the current fluctuations in background pressure

  14. Nonequilibrium fluctuations in a resistor.

    Science.gov (United States)

    Garnier, N; Ciliberto, S

    2005-06-01

    In small systems where relevant energies are comparable to thermal agitation, fluctuations are of the order of average values. In systems in thermodynamical equilibrium, the variance of these fluctuations can be related to the dissipation constant in the system, exploiting the fluctuation-dissipation theorem. In nonequilibrium steady systems, fluctuations theorems (FT) additionally describe symmetry properties of the probability density functions (PDFs) of the fluctuations of injected and dissipated energies. We experimentally probe a model system: an electrical dipole driven out of equilibrium by a small constant current I, and show that FT are experimentally accessible and valid. Furthermore, we stress that FT can be used to measure the dissipated power P = R I2 in the system by just studying the PDFs' symmetries.

  15. Suppressing Quantum Fluctuations in Classicalization

    CERN Document Server

    Vikman, Alexander

    2013-01-01

    We study vacuum quantum fluctuations of simple Nambu-Goldstone bosons - derivatively coupled single scalar-field theories possessing shift-symmetry in field space. We argue that quantum fluctuations of the interacting field can be drastically suppressed with respect to the free-field case. Moreover, the power-spectrum of these fluctuations can soften to become red for sufficiently small scales. In quasiclassical approximation, we demonstrate that this suppression can only occur for those theories that admit such classical static backgrounds around which small perturbations propagate faster than light. Thus a quasiclassical softening of quantum fluctuations is only possible for theories which classicalize instead of having a usual Lorentz invariant and local Wilsonian UV- completion. We illustrate our analysis by estimating the quantum fluctuations for the DBI-like theories.

  16. Gravitons and light cone fluctuations

    International Nuclear Information System (INIS)

    Ford, L.H.

    1995-01-01

    Gravitons in a squeezed vacuum state, the natural result of quantum creation in the early Universe or by black holes, will introduce metric fluctuations. These metric fluctuations will introduce fluctuations of the light cone. It is shown that when the various two-point functions of a quantized field are averaged over the metric fluctuations, the light cone singularity disappears for distinct points. The metric-averaged functions remain singular in the limit of coincident points. The metric-averaged retarded Green's function for a massless field becomes a Gaussian which is nonzero both inside and outside of the classical light cone. This implies some photons propagate faster than the classical light speed, whereas others propagate slower. The possible effects of metric fluctuations upon one-loop quantum processes are discussed and illustrated by the calculation of the one-loop electron self-energy

  17. Quantum fluctuations from thermal fluctuations in Jacobson formalism

    Energy Technology Data Exchange (ETDEWEB)

    Faizal, Mir [University of British Columbia-Okanagan, Irving K. Barber School of Arts and Sciences, Kelowna, BC (Canada); University of Lethbridge, Department of Physics and Astronomy, Lethbridge, AB (Canada); Ashour, Amani; Alcheikh, Mohammad [Damascus University, Mathematics Department, Faculty of Science, Damascus (Syrian Arab Republic); Alasfar, Lina [Universite Clermont Auvergne, Laboratoire de Physique Corpusculaire de Clermont-Ferrand, Aubiere (France); Alsaleh, Salwa; Mahroussah, Ahmed [King Saud University, Department of Physics and Astronomy, Riyadh (Saudi Arabia)

    2017-09-15

    In the Jacobson formalism general relativity is obtained from thermodynamics. This is done by using the Bekenstein-Hawking entropy-area relation. However, as a black hole gets smaller, its temperature will increase. This will cause the thermal fluctuations to also increase, and these will in turn correct the Bekenstein-Hawking entropy-area relation. Furthermore, with the reduction in the size of the black hole, quantum effects will also start to dominate. Just as the general relativity can be obtained from thermodynamics in the Jacobson formalism, we propose that the quantum fluctuations to the geometry can be obtained from thermal fluctuations. (orig.)

  18. Effects of dynamic redox zonation on the potential for natural attenuation of trichloroethylene at a fire-training-impacted aquifer

    Science.gov (United States)

    Skubal, K.L.; Haack, S.K.; Forney, L.J.; Adriaens, P.

    1999-01-01

    Hydrogeochemical and microbiological methods were used to characterize temporal changes along a transect of an aquifer contaminated by mixed hydrocarbon and solvent wastes from fire training activities at Wurtsmith Air Force Base (Oscoda, MI). Predominant terminal electron accepting processes (TEAPs) as measured by dissolved hydrogen indicated reoxygenation along the transect between October 1995 and October 1996, possibly because of recharge, fluctuations in water table elevation, or microbial activity. Microbiological analyses using universal and archaeal probes revealed a relationship between groundwater hydrogen concentration, TEAP, and predominant bacterial phylogeny. Specifically, a raised water table level and evidence of methanogenesis corresponded to an order of magnitude increase in archaeal 16S rRNA relative to when this zone was unsaturated. Spatial microbial and geochemical dynamics did not result in measurable differences in trichloroethylene (TCE) mineralization potential in vadose, capillary fringe, and saturated zone soils during a 500-day microcosm experiment using unprocessed contaminated soil and groundwater. Aerobic systems indicated that methane, but not toluene, may serve as cosubstrate for TCE cometabolism. Anaerobic microcosms demonstrated evidence for methanogenesis, CO2 production and hydrogen consumption, yet dechlorination activity was only observed in a microcosm with sulfate-reduction as the dominant TEAP. Mass balance calculations indicated less than 5% mineralization, regardless of redox zone or degree of saturation, at maximum rates of 0.01-0.03 ??mol/g soil??d. The general lack of dechlorination activity under laboratory conditions corroborates the limited evidence for natural dechlorination at this site, despite abundant electron donor material and accumulated organic acids from microbial degradation of alkylbenzenes. Thus, the short-term temporal dynamics in redox conditions is unlikely to have measurable effects on the long

  19. Redox pioneer: professor Barry Halliwell.

    Science.gov (United States)

    Pervaiz, Shazib

    2011-05-01

    Professor Barry Halliwell is recognized as a Redox Pioneer because he has published eight articles on redox biology that have been each cited more than 1000 times, and 158 articles that have been each cited more than 100 times. His contributions go back as far as 1976, when he was involved in elucidation of the Foyer-Halliwell-Asada cycle, an efficient mechanism for preventing oxidative damage to chloroplasts. His subsequent work established the important role of iron and zinc in free radical reactions and their relevance to human pathologies. Professor Halliwell is also a leader in developing novel methodology for detecting free radical intermediates in vivo, and his contributions to our knowledge of reactive nitrogen species are highly significant. His sustained excellence won him the top-cited scientist award in the United Kingdom in biomedical sciences in 1999, and in 2003 he was recognized as a highly cited scientist by Institute of Scientific Information (ISI) for work on plant antioxidants, and the same year ranked 28 out of 5494 biochemists/biologists for scientific impact. Two pieces of his scholarly work have been listed as Citation Classics by ISI, and in 2007 his laboratory was ranked number 1 worldwide based on highest citation score in research on free radicals.

  20. Information processing through a bio-based redox capacitor: signatures for redox-cycling.

    Science.gov (United States)

    Liu, Yi; Kim, Eunkyoung; White, Ian M; Bentley, William E; Payne, Gregory F

    2014-08-01

    Redox-cycling compounds can significantly impact biological systems and can be responsible for activities that range from pathogen virulence and contaminant toxicities, to therapeutic drug mechanisms. Current methods to identify redox-cycling activities rely on the generation of reactive oxygen species (ROS), and employ enzymatic or chemical methods to detect ROS. Here, we couple the speed and sensitivity of electrochemistry with the molecular-electronic properties of a bio-based redox-capacitor to generate signatures of redox-cycling. The redox capacitor film is electrochemically-fabricated at the electrode surface and is composed of a polysaccharide hydrogel with grafted catechol moieties. This capacitor film is redox-active but non-conducting and can engage diffusible compounds in either oxidative or reductive redox-cycling. Using standard electrochemical mediators ferrocene dimethanol (Fc) and Ru(NH3)6Cl3 (Ru(3+)) as model redox-cyclers, we observed signal amplifications and rectifications that serve as signatures of redox-cycling. Three bio-relevant compounds were then probed for these signatures: (i) ascorbate, a redox-active compound that does not redox-cycle; (ii) pyocyanin, a virulence factor well-known for its reductive redox-cycling; and (iii) acetaminophen, an analgesic that oxidatively redox-cycles but also undergoes conjugation reactions. These studies demonstrate that the redox-capacitor can enlist the capabilities of electrochemistry to generate rapid and sensitive signatures of biologically-relevant chemical activities (i.e., redox-cycling). Published by Elsevier B.V.

  1. Methods of simulating low redox potential (Eh) for a basalt repository

    International Nuclear Information System (INIS)

    Jantzen, C.M.

    1983-01-01

    Basalt groundwaters have inherently low redox potentials, approximately -0.4V, which can be measured with platinum electrodes, but are difficult to reproduce during leaching experiments. In the presence of deionized water, crushed basalt reaches the measured Eh-pH values of a basalt repository. Other waste package components, such as iron, will interact with groundwater in different ways under oxic or anoxic conditions since the presence of any redox active solid will affect the groundwater Eh. 26 references, 4 figures

  2. Characterization of redox proteins using electrochemical methods

    NARCIS (Netherlands)

    Verhagen, M.

    1995-01-01

    The use of electrochemical techniques in combination with proteins started approximately a decade ago and has since then developed into a powerfull technique for the study of small redox proteins. In addition to the determination of redox potentials, electrochemistry can be used to obtain

  3. Interplay between redox status and inflammasome activation

    NARCIS (Netherlands)

    Rubartelli, A.; Gattorno, M.; Netea, M.G.; Dinarello, C.A.

    2011-01-01

    Several inflammation-related processes, including inflammasome activation and interleukin (IL)-1beta secretion, are dependent on redox signaling. However, the type of redox response involved as well as the relevant role of pro-oxidant and antioxidant events are matters of intense debate. By

  4. Redox and Reactive Oxygen Species Regulation of Mitochondrial Cytochrome c Oxidase Biogenesis

    Science.gov (United States)

    Bourens, Myriam; Fontanesi, Flavia; Soto, Iliana C.; Liu, Jingjing

    2013-01-01

    Abstract Significance: Cytochrome c oxidase (COX), the last enzyme of the mitochondrial respiratory chain, is the major oxygen consumer enzyme in the cell. COX biogenesis involves several redox-regulated steps. The process is highly regulated to prevent the formation of pro-oxidant intermediates. Recent Advances: Regulation of COX assembly involves several reactive oxygen species and redox-regulated steps. These include: (i) Intricate redox-controlled machineries coordinate the expression of COX isoenzymes depending on the environmental oxygen concentration. (ii) COX is a heme A-copper metalloenzyme. COX copper metallation involves the copper chaperone Cox17 and several other recently described cysteine-rich proteins, which are oxidatively folded in the mitochondrial intermembrane space. Copper transfer to COX subunits 1 and 2 requires concomitant transfer of redox power. (iii) To avoid the accumulation of reactive assembly intermediates, COX is regulated at the translational level to minimize synthesis of the heme A-containing Cox1 subunit when assembly is impaired. Critical Issues: An increasing number of regulatory pathways converge to facilitate efficient COX assembly, thus preventing oxidative stress. Future Directions: Here we will review on the redox-regulated COX biogenesis steps and will discuss their physiological relevance. Forthcoming insights into the precise regulation of mitochondrial COX biogenesis in normal and stress conditions will likely open future perspectives for understanding mitochondrial redox regulation and prevention of oxidative stress. Antioxid. Redox Signal. 19, 1940–1952. PMID:22937827

  5. [Effect of the medium redox potential on the growth and metabolism of anaerobic bacteria].

    Science.gov (United States)

    Vasilian, A; Trchunian, A

    2008-01-01

    Based on the available literature data on a decrease in the redox potential of medium to low negative values and a decrease in pH during the growth of sugar-fermenting anaerobic bacteria, it was concluded that these processes cannot be described by the theory of redox potential. A theory was developed according to which the regulation of bacterial metabolism is accomplished through changes in the redox potential. The theory considers the redox potential as a factor determining the growth of anaerobic bacteria, which is regulated by oxidizers and reducers. The assumption is put forward that, under anaerobic conditions, bacteria are sensitive to changes in the redox potential and have a redox taxis. The effect of the redox potential on the transport of protons and other substances through membranes and the activity of membrane-bound enzymes, including the proton F1-F0-ATPase, whose mechanisms of action involve changes in the proton conductance of the membrane, the generation of proton-driving force, and dithiol-disulfide transitions in proteins was studied.

  6. Spectral fluctuations and zeta functions

    International Nuclear Information System (INIS)

    Balazs, N.L.; Schmit, C.; Voros, A.

    1987-01-01

    The study theoretically and numerically the role of the fluctuations of eigenvalue spectra {μ/sub n} in a particular analytical continuation process applied to the (generalized) zeta function Z(s) = Σ/sub n/μ/sub n//sup -s/ for s large and positive. A particularly interesting example is the spectrum of the Laplacian on a triangular domain which tessellates a compact surface of constant negative curvature (of genus two). The authors indeed find that the fluctuations restrict the abscissa of convergence, and also affect the rate of convergence. This then initiates a new approach to the exploration of spectral fluctuations through the convergence of analytical continuation processes

  7. Fluctuation theorem: A critical review

    Science.gov (United States)

    Malek Mansour, M.; Baras, F.

    2017-10-01

    Fluctuation theorem for entropy production is revisited in the framework of stochastic processes. The applicability of the fluctuation theorem to physico-chemical systems and the resulting stochastic thermodynamics were analyzed. Some unexpected limitations are highlighted in the context of jump Markov processes. We have shown that these limitations handicap the ability of the resulting stochastic thermodynamics to correctly describe the state of non-equilibrium systems in terms of the thermodynamic properties of individual processes therein. Finally, we considered the case of diffusion processes and proved that the fluctuation theorem for entropy production becomes irrelevant at the stationary state in the case of one variable systems.

  8. The Mineralogy, Geochemistry, and Redox State of Multivalent Cations During the Crystallization of Primitive Shergottitic Liquids at Various (f)O2. Insights into the (f)O2 Fugacity of the Martian Mantle and Crustal Influences on Redox Conditions of Martian Magmas.

    Science.gov (United States)

    Shearer, C. K.; Bell, A. S.; Burger, P. V.; Papike, J. J.; Jones, J.; Le, L.; Muttik, N.

    2016-01-01

    The (f)O2 [oxygen fugacity] of crystallization for martian basalts has been estimated in various studies to range from IW-1 to QFM+4 [1-3]. A striking geochemical feature of the shergottites is the large range in initial Sr isotopic ratios and initial epsilon(sup Nd) values. Studies by observed that within the shergottite group the (f)O2 [oxygen fugacity] of crystallization is highly correlated with these chemical and isotopic characteristics with depleted shergottites generally crystallizing at reduced conditions and enriched shergottites crystallizing under more oxidizing conditions. More recent work has shown that (f)O2 [oxygen fugacity] changed during the crystallization of these magmas from one order of magnitude in Y980459 (Y98) to several orders of magnitude in Larkman Nunatak 06319. These real or apparent variations within single shergottitic magmas have been attributed to mixing of a xenocrystic olivine component, volatile loss-water disassociation, auto-oxidation during crystallization of mafic phases, and assimilation of an oxidizing crustal component (e.g. sulfate). In contrast to the shergottites, augite basalts such as NWA 8159 are highly depleted yet appear to be highly oxidized (e.g. QFM+4). As a first step in attempting to unravel petrologic complexities that influence (f)O2 [oxygen fugacity] in martian magmas, this study explores the effect of (f)O2 [oxygen fugacity] on the liquid line of descent (LLD) for a primitive shergottite liquid composition (Y98). The results of this study will provide a fundamental basis for reconstructing the record of (f)O2 [oxygen fugacity] in shergottites and other martian basalts, its effect on both mineral chemistries and valence state partitioning, and a means for examining the role of crystallization (and other more complex processes) on the petrologic linkages between olivine-phyric and pyroxene-plagioclase shergottites.

  9. Microbial Response to Experimentally Controlled Redox Transitions at the Sediment Water Interface

    Science.gov (United States)

    Frindte, Katharina; Allgaier, Martin; Grossart, Hans-Peter; Eckert, Werner

    2015-01-01

    The sediment–water interface of freshwater lakes is characterized by sharp chemical gradients, shaped by the interplay between physical, chemical and microbial processes. As dissolved oxygen is depleted in the uppermost sediment, the availability of alternative electron acceptors, e.g. nitrate and sulfate, becomes the limiting factor. We performed a time series experiment in a mesocosm to simulate the transition from aerobic to anaerobic conditions at the sediment–water interface. Our goal was to identify changes in the microbial activity due to redox transitions induced by successive depletion of available electron acceptors. Monitoring critical hydrochemical parameters in the overlying water in conjunction with a new sampling strategy for sediment bacteria enabled us to correlate redox changes in the water to shifts in the active microbial community and the expression of functional genes representing specific redox-dependent microbial processes. Our results show that during several transitions from oxic-heterotrophic condition to sulfate-reducing condition, nitrate-availability and the on-set of sulfate reduction strongly affected the corresponding functional gene expression. There was evidence of anaerobic methane oxidation with NOx. DGGE analysis revealed redox-related changes in microbial activity and expression of functional genes involved in sulfate and nitrite reduction, whereas methanogenesis and methanotrophy showed only minor changes during redox transitions. The combination of high-frequency chemical measurements and molecular methods provide new insights into the temporal dynamics of the interplay between microbial activity and specific redox transitions at the sediment–water interface. PMID:26599000

  10. Engineered Proteins: Redox Properties and Their Applications

    Science.gov (United States)

    Prabhulkar, Shradha; Tian, Hui; Wang, Xiaotang; Zhu, Jun-Jie

    2012-01-01

    Abstract Oxidoreductases and metalloproteins, representing more than one third of all known proteins, serve as significant catalysts for numerous biological processes that involve electron transfers such as photosynthesis, respiration, metabolism, and molecular signaling. The functional properties of the oxidoreductases/metalloproteins are determined by the nature of their redox centers. Protein engineering is a powerful approach that is used to incorporate biological and abiological redox cofactors as well as novel enzymes and redox proteins with predictable structures and desirable functions for important biological and chemical applications. The methods of protein engineering, mainly rational design, directed evolution, protein surface modifications, and domain shuffling, have allowed the creation and study of a number of redox proteins. This review presents a selection of engineered redox proteins achieved through these methods, resulting in a manipulation in redox potentials, an increase in electron-transfer efficiency, and an expansion of native proteins by de novo design. Such engineered/modified redox proteins with desired properties have led to a broad spectrum of practical applications, ranging from biosensors, biofuel cells, to pharmaceuticals and hybrid catalysis. Glucose biosensors are one of the most successful products in enzyme electrochemistry, with reconstituted glucose oxidase achieving effective electrical communication with the sensor electrode; direct electron-transfer-type biofuel cells are developed to avoid thermodynamic loss and mediator leakage; and fusion proteins of P450s and redox partners make the biocatalytic generation of drug metabolites possible. In summary, this review includes the properties and applications of the engineered redox proteins as well as their significance and great potential in the exploration of bioelectrochemical sensing devices. Antioxid. Redox Signal. 17, 1796–1822. PMID:22435347

  11. The Fluctuation Niche in Plants

    International Nuclear Information System (INIS)

    Terradas, J.; Penuelas, J.; Lloret, F.; Penuelas, J.

    2009-01-01

    Classical approaches to niche in coexisting plants have undervalued temporal fluctuations. We propose that fluctuation niche is an important dimension of the total niche and interacts with habitat and life-history niches to provide a better understanding of the multidimensional niche space where ecological interactions occur. To scale a fluctuation niche, it is necessary to relate environmental constrictions or species performance not only to the absolute values of the usual environmental and eco physiological variables but also to their variances or other measures of variability. We use Mediterranean plant communities as examples, because they present characteristic large seasonal and inter annual fluctuations in water and nutrient availabilities, along an episodic-constant gradient, and because the plant responses include a number of syndromes coupled to this gradient.

  12. The Fluctuation Niche in Plants

    Directory of Open Access Journals (Sweden)

    Jaume Terradas

    2009-01-01

    Full Text Available Classical approaches to niche in coexisting plants have undervalued temporal fluctuations. We propose that fluctuation niche is an important dimension of the total niche and interacts with habitat and life-history niches to provide a better understanding of the multidimensional niche space where ecological interactions occur. To scale a fluctuation niche, it is necessary to relate environmental constrictions or species performance not only to the absolute values of the usual environmental and ecophysiological variables but also to their variances or other measures of variability. We use Mediterranean plant communities as examples, because they present characteristic large seasonal and interannual fluctuations in water and nutrient availabilities, along an episodic-constant gradient, and because the plant responses include a number of syndromes coupled to this gradient.

  13. Molecular evolution under fitness fluctuations.

    Science.gov (United States)

    Mustonen, Ville; Lässig, Michael

    2008-03-14

    Molecular evolution is a stochastic process governed by fitness, mutations, and reproductive fluctuations in a population. Here, we study evolution where fitness itself is stochastic, with random switches in the direction of selection at individual genomic loci. As the correlation time of these fluctuations becomes larger than the diffusion time of mutations within the population, fitness changes from an annealed to a quenched random variable. We show that the rate of evolution has its maximum in the crossover regime, where both time scales are comparable. Adaptive evolution emerges in the quenched fitness regime (evidence for such fitness fluctuations has recently been found in genomic data). The joint statistical theory of reproductive and fitness fluctuations establishes a conceptual connection between evolutionary genetics and statistical physics of disordered systems.

  14. Fluctuating attention in Parkinson's disease

    DEFF Research Database (Denmark)

    Starrfelt, Randi; Aarsland, Dag; Janvin, Carmen

    2001-01-01

    great variability in performance in reactiontime tasks. Aiming to investigate fluctuation of attention in PD, we re- analysed data from a cue-target reactiontime task, specifically searching for differences in variability between patients and controls. The subjects included were a representative group...... a significant difference (pattention might be fluctuating on a moment to moment basis in PD. Some of the PD patients have also been tested with a choice reaction time...... task, shown by Walker et al. (2000) to be sensi- tive to fluctuation of cognition in DLB patients. Preliminary data-analysis indicate that PD patients also show considerable intra-individual variation in performance on this test. These findings suggest that fluctuating attention and cogni- tion...

  15. Fluctuations and Instability in Sedimentation

    KAUST Repository

    Guazzelli, Élisabeth

    2011-01-21

    This review concentrates on the fluctuations of the velocities of sedimenting spheres, and on the structural instability of a suspension of settling fibers. For many years, theoretical estimates and numerical simulations predicted the fluctuations of the velocities of spheres to increase with the size of the container, whereas experiments found no such variation. Two ideas have increased our understanding. First, the correlation length of the velocity fluctuations was found experimentally to be 20 interparticle separations. Second, in dilute suspensions, a vertical variation in the concentration due to the spreading of the front with the clear fluid can inhibit the velocity fluctuations. In a very dilute regime, a homogeneous suspension of fibers suffers a spontaneous instability in which fast descending fiber-rich columns are separated by rising fiber-sparse columns. In a semidilute regime, the settling is hindered, more so than for spheres. © 2011 by Annual Reviews. All rights reserved.

  16. Collective fluctuations in magnetized plasma: Transition probability approach

    International Nuclear Information System (INIS)

    Sosenko, P.P.

    1997-01-01

    Statistical plasma electrodynamics is elaborated with special emphasis on the transition probability approach and quasi-particles, and on modern applications to magnetized plasmas. Fluctuation spectra in the magnetized plasma are calculated in the range of low frequencies (with respect to the cyclotron one), and the conditions for the transition from incoherent to collective fluctuations are established. The role of finite-Larmor-radius effects and particle polarization drift in such a transition is explained. The ion collective features in fluctuation spectra are studied. 63 refs., 30 figs

  17. Enhanced microbial decolorization of methyl red with oxidized carbon fiber as redox mediator

    International Nuclear Information System (INIS)

    Emilia Rios-Del Toro, E.; Celis, Lourdes B.; Cervantes, Francisco J.; Rangel-Mendez, J. Rene

    2013-01-01

    Highlights: • Activated carbon fibers (ACFs) act as redox mediator. • Electron accepting capacity increased with oxidation time of ACF. •ACFs increased 8-fold the reduction of methyl red in biological assays. •Biofilm formed on the ACFs partly blocked their redox mediator capacity. -- Abstract: The anaerobic degradation of azo dyes under anaerobic conditions is possible but at a slow rate. Redox mediators (quinones, activated carbon) are used to improve the reduction rate. The aim of this work was to use activated carbon fiber (ACF) as a redox mediator for the anaerobic reduction of the azo dye methyl red. ACF was chemically modified with 8 M HNO 3 to increase its redox-mediating capacity and used in chemical and anaerobic biological batch assays for the reduction of methyl red. ACF increased its redox-mediating capacity up to 3-fold in chemical assays; in biological assays ACF increased the reduction rate up to 8-fold compared to controls without ACF. However, since the ACF served as support for biomass, a biofilm formed on the fiber significantly reduced its redox-mediating capacity; substrate consumption suggested that the electron transport from ACF to methyl red was the rate-limiting step in the process. These results are the first evidence of the role of ACF as a redox mediator in the reductive decolorization of methyl red, in addition to the effect of biofilm attached to ACF on methyl red reduction. Due to the versatile characteristics of ACF and its redox-mediating capacity, carbon fibers could be used in biological wastewater treatment systems to accelerate the reductive transformation of pollutants commonly found in industrial effluents

  18. Enhanced microbial decolorization of methyl red with oxidized carbon fiber as redox mediator

    Energy Technology Data Exchange (ETDEWEB)

    Emilia Rios-Del Toro, E. [División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Sección, San Luis Potosí, SLP 78216 (Mexico); Celis, Lourdes B. [División de Geociencias Aplicadas, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Sección, San Luis Potosí, SLP 78216 (Mexico); Cervantes, Francisco J. [División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Sección, San Luis Potosí, SLP 78216 (Mexico); Rangel-Mendez, J. Rene, E-mail: rene@ipicyt.edu.mx [División de Ciencias Ambientales, Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), Camino a la Presa San José 2055, Col. Lomas 4a Sección, San Luis Potosí, SLP 78216 (Mexico)

    2013-09-15

    Highlights: • Activated carbon fibers (ACFs) act as redox mediator. • Electron accepting capacity increased with oxidation time of ACF. •ACFs increased 8-fold the reduction of methyl red in biological assays. •Biofilm formed on the ACFs partly blocked their redox mediator capacity. -- Abstract: The anaerobic degradation of azo dyes under anaerobic conditions is possible but at a slow rate. Redox mediators (quinones, activated carbon) are used to improve the reduction rate. The aim of this work was to use activated carbon fiber (ACF) as a redox mediator for the anaerobic reduction of the azo dye methyl red. ACF was chemically modified with 8 M HNO{sub 3} to increase its redox-mediating capacity and used in chemical and anaerobic biological batch assays for the reduction of methyl red. ACF increased its redox-mediating capacity up to 3-fold in chemical assays; in biological assays ACF increased the reduction rate up to 8-fold compared to controls without ACF. However, since the ACF served as support for biomass, a biofilm formed on the fiber significantly reduced its redox-mediating capacity; substrate consumption suggested that the electron transport from ACF to methyl red was the rate-limiting step in the process. These results are the first evidence of the role of ACF as a redox mediator in the reductive decolorization of methyl red, in addition to the effect of biofilm attached to ACF on methyl red reduction. Due to the versatile characteristics of ACF and its redox-mediating capacity, carbon fibers could be used in biological wastewater treatment systems to accelerate the reductive transformation of pollutants commonly found in industrial effluents.

  19. Primordial fluctuations from nonlinear couplings

    International Nuclear Information System (INIS)

    Calzetta, E.A.; Gonorazky, S.

    1997-01-01

    We study the spectrum of primordial fluctuations in theories where the inflaton field is nonlinearly coupled to massless fields and/or to itself. Conformally invariant theories generically predict a scale-invariant spectrum. Scales entering the theory through infrared divergences cause logarithmic corrections to the spectrum, tilting it towards the blue. We discuss in some detail whether these fluctuations are quantum or classical in nature. copyright 1997 The American Physical Society

  20. Literature survey of redox reactions in the near field

    International Nuclear Information System (INIS)

    Miki, Takahito; Chiba, Tamotsu; Inagaki, Manabu; Sasamoto, Hiroshi; Yui, Mikazu

    2000-01-01

    This report presents a summary of literature survey about geochemical reactions which are important to evaluate the redox conditions in the near field rock mass and buffer. The results of literature survey are summarized as follows; Minerals including ferrous iron and organic materials in the rock mass are important reductants. Initial stage after closure of repository, oxygen will be consumed by pyrite, because the reaction rate between pyrite and oxygen is relatively fast. It is possible to estimate the redox capacity for reductants by rock (mineral)-water interaction experiment in a laboratory. And it is expected that the ferrous iron-rich rock and higher porosity rock may have bigger redox capacity. It is impossible to estimate the oxygen consumption rate by reductants such as minerals including ferrous iron. The rate law and rate constant for the oxidation reaction of ferrous iron in the solution are also determined. As a conclusion, it seems that we can evaluate kinetically the evolution of geochemical conditions in the near field rock mass and buffer by excavation of drifts, based on data derived from these existing literatures. (author)

  1. Chiral cyclopentadienylruthenium sulfoxide catalysts for asymmetric redox bicycloisomerization

    Directory of Open Access Journals (Sweden)

    Barry M. Trost

    2016-06-01

    Full Text Available A full account of our efforts toward an asymmetric redox bicycloisomerization reaction is presented in this article. Cyclopentadienylruthenium (CpRu complexes containing tethered chiral sulfoxides were synthesized via an oxidative [3 + 2] cycloaddition reaction between an alkyne and an allylruthenium complex. Sulfoxide complex 1 containing a p-anisole moiety on its sulfoxide proved to be the most efficient and selective catalyst for the asymmetric redox bicycloisomerization of 1,6- and 1,7-enynes. This complex was used to synthesize a broad array of [3.1.0] and [4.1.0] bicycles. Sulfonamide- and phosphoramidate-containing products could be deprotected under reducing conditions. Catalysis performed with enantiomerically enriched propargyl alcohols revealed a matched/mismatched effect that was strongly dependent on the nature of the solvent.

  2. Glutathione in Cellular Redox Homeostasis: Association with the Excitatory Amino Acid Carrier 1 (EAAC1

    Directory of Open Access Journals (Sweden)

    Koji Aoyama

    2015-05-01

    Full Text Available Reactive oxygen species (ROS are by-products of the cellular metabolism of oxygen consumption, produced mainly in the mitochondria. ROS are known to be highly reactive ions or free radicals containing oxygen that impair redox homeostasis and cellular functions, leading to cell death. Under physiological conditions, a variety of antioxidant systems scavenge ROS to maintain the intracellular redox homeostasis and normal cellular functions. This review focuses on the antioxidant system’s roles in maintaining redox homeostasis. Especially, glutathione (GSH is the most important thiol-containing molecule, as it functions as a redox buffer, antioxidant, and enzyme cofactor against oxidative stress. In the brain, dysfunction of GSH synthesis leading to GSH depletion exacerbates oxidative stress, which is linked to a pathogenesis of aging-related neurodegenerative diseases. Excitatory amino acid carrier 1 (EAAC1 plays a pivotal role in neuronal GSH synthesis. The regulatory mechanism of EAAC1 is also discussed.

  3. Sawteeth fluctuations detection

    International Nuclear Information System (INIS)

    Vannucci, A.; Nascimento, I.C. do; Caldas, I.L.; Sanada, E.K.; Tuszel, A.G.; Fagundes, A.N.

    1988-01-01

    Sawteeth oscillations were identified during the discharges in the small Tokamak TBR-1 through a pin-hole camera and their main characteristics were studied. Comparing the measured period of the internal disruption (sawteeth) with the ones expected from scaling laws, good agreement is reached. The measured sawteeth crashes agree with the value expected from Kadomtsev's model. However, there some sawteeth oscillations, corresponding to conditions of higher Z eff of the plasma, which showed longer crashes that could not be explained by this model. (author) [pt

  4. Redox activity of naphthalene secondary organic aerosol

    OpenAIRE

    R. D. McWhinney; S. Zhou; J. P. D. Abbatt

    2013-01-01

    Chamber secondary organic aerosol (SOA) from low-NOx photooxidation of naphthalene by hydroxyl radical was examined with respect to its redox cycling behaviour using the dithiothreitol (DTT) assay. Naphthalene SOA was highly redox active, consuming DTT at an average rate of 118 ± 14 pmol per minute per μg of SOA material. Measured particle-phase masses of the major previously identified redox active products, 1,2- and 1,4-naphthoquinone, accounted for only 21 ± 3% of the obse...

  5. Diffusion by Infragravity Stokes Drift Fluctuations

    Science.gov (United States)

    Smit, P.; Janssen, T. T.; Herbers, T. H. C.; Kirshner, Z.

    2016-02-01

    The group-scale variability of ocean waves variability drives infragravity Stokes drift fluctuations, which are important for small-scale diffusion of passive tracers (to the order of a few kilometers), and can thus be important for the break-up and dispersion of e.g. oil spills or sewage outflow, and coastal transport in general. The implications of this were first considered theoretically by Herterich and Hasselmann (1982, JPO), who demonstrated that on small scales, wave diffusion can compete with other upper ocean diffusive processes, but their theory has thus far not been extensively validated with field observations. To investigate drift fluctuations and wave-induced diffusion, we consider the wave-induced dispersion of a cluster of O(10) buoys. The experiment, conducted offshore of San Francisco, uses a cluster of Lagrangian drifters equipped with fast-sampling GPS sensor packages, to accurately resolve both the surface wave motions, and directly measure the Lagrangian dynamics, including surface drift fluctuations. We revisit the Herterich and Hasselmann theory, expand it to include shallow water and variable wave conditions, and compare the theoretical predictions with the new observations.

  6. Analysis of Meniscus Fluctuation in a Continuous Casting Slab Mold

    Science.gov (United States)

    Zhang, Kaitian; Liu, Jianhua; Cui, Heng; Xiao, Chao

    2018-03-01

    A water model of slab mold was established to analyze the microscopic and macroscopic fluctuation of meniscus. The fast Fourier transform and wavelet entropy were adopted to analyze the wave amplitude, frequency, and components of fluctuation. The flow patterns under the meniscus were measured by using particle image velocimetry measurement and then the mechanisms of meniscus fluctuation were discussed. The results reflected that wavelet entropy had multi-scale and statistical properties, and it was suitable for the study of meniscus fluctuation details both in time and frequency domain. The basic wave, frequency of which exceeding 1 Hz in the condition of no mold oscillation, was demonstrated in this work. In fact, three basic waves were found: long-wave with low frequency, middle-wave with middle frequency, and short-wave with high frequency. In addition, the upper roll flow in mold had significant effect on meniscus fluctuation. When the position of flow impinged was far from the meniscus, long-wave dominated the fluctuation and the stability of meniscus was enhanced. However, when the velocity of flow was increased, the short-wave dominated the meniscus fluctuation and the meniscus stability was decreased.

  7. Determining the Limiting Current Density of Vanadium Redox Flow Batteries

    Directory of Open Access Journals (Sweden)

    Jen-Yu Chen

    2014-09-01

    Full Text Available All-vanadium redox flow batteries (VRFBs are used as energy storage systems for intermittent renewable power sources. The performance of VRFBs depends on materials of key components and operating conditions, such as current density, electrolyte flow rate and electrolyte composition. Mass transfer overpotential is affected by the electrolyte flow rate and electrolyte composition, which is related to the limiting current density. In order to investigate the effect of operating conditions on mass transport overpotential, this study established a relationship between the limiting current density and operating conditions. First, electrolyte solutions with different states of charge were prepared and used for a single cell to obtain discharging polarization curves under various operating conditions. The experimental results were then analyzed and are discussed in this paper. Finally, this paper proposes a limiting current density as a function of operating conditions. The result helps predict the effect of operating condition on the cell performance in a mathematical model.

  8. Species-Specific Thiol-Disulfide Equilibrium Constant: A Tool To Characterize Redox Transitions of Biological Importance.

    Science.gov (United States)

    Mirzahosseini, Arash; Somlyay, Máté; Noszál, Béla

    2015-08-13

    Microscopic redox equilibrium constants, a new species-specific type of physicochemical parameters, were introduced and determined to quantify thiol-disulfide equilibria of biological significance. The thiol-disulfide redox equilibria of glutathione with cysteamine, cysteine, and homocysteine were approached from both sides, and the equilibrium mixtures were analyzed by quantitative NMR methods to characterize the highly composite, co-dependent acid-base and redox equilibria. The directly obtained, pH-dependent, conditional constants were then decomposed by a new evaluation method, resulting in pH-independent, microscopic redox equilibrium constants for the first time. The 80 different, microscopic redox equilibrium constant values show close correlation with the respective thiolate basicities and provide sound means for the development of potent agents against oxidative stress.

  9. Redox Modulations, Antioxidants, and Neuropsychiatric Disorders

    Science.gov (United States)

    Fraunberger, Erik A.; Laliberté, Victoria L. M.; Duong, Angela; Andreazza, Ana C.

    2016-01-01

    Although antioxidants, redox modulations, and neuropsychiatric disorders have been widely studied for many years, the field would benefit from an integrative and corroborative review. Our primary objective is to delineate the biological significance of compounds that modulate our redox status (i.e., reactive species and antioxidants) as well as outline their current role in brain health and the impact of redox modulations on the severity of illnesses. Therefore, this review will not enter into the debate regarding the perceived medical legitimacy of antioxidants but rather seek to clarify their abilities and limitations. With this in mind, antioxidants may be interpreted as natural products with significant pharmacological actions in the body. A renewed understanding of these often overlooked compounds will allow us to critically appraise the current literature and provide an informed, novel perspective on an important healthcare issue. In this review, we will introduce the complex topics of redox modulations and their role in the development of select neuropsychiatric disorders. PMID:26640614

  10. Mitochondrial redox biology and homeostasis in plants.

    Science.gov (United States)

    Noctor, Graham; De Paepe, Rosine; Foyer, Christine H

    2007-03-01

    Mitochondria are key players in plant cell redox homeostasis and signalling. Earlier concepts that regarded mitochondria as secondary to chloroplasts as the powerhouses of photosynthetic cells, with roles in cell proliferation, death and ageing described largely by analogy to animal paradigms, have been replaced by the new philosophy of integrated cellular energy and redox metabolism involving mitochondria and chloroplasts. Thanks to oxygenic photosynthesis, plant mitochondria often operate in an oxygen- and carbohydrate-rich environment. This rather unique environment necessitates extensive flexibility in electron transport pathways and associated NAD(P)-linked enzymes. In this review, mitochondrial redox metabolism is discussed in relation to the integrated cellular energy and redox function that controls plant cell biology and fate.

  11. Symproportionation versus Disproportionation in Bromine Redox Systems

    International Nuclear Information System (INIS)

    Toporek, Marcin; Michałowska-Kaczmarczyk, Anna M.; Michałowski, Tadeusz

    2015-01-01

    Graphical abstract: Display Omitted -- Highlights: • The disproportionation and symproportionation of bromine in different media is presented. • All the redox systems are elaborated according to the principles of the generalized approach to electrolytic redox systems (GATES/GEB). • All physicochemical knowledge is involved in the algorithm applied for this purpose. • The graphical representation of the systems is the basis of gaining the detailed physicochemical knowledge on the systems in question. -- Abstract: The paper refers to dynamic (titration) redox systems where symproportionation or disproportionation of bromine species occur. The related systems are modeled according to principles assumed in the Generalized Approach to Electrolytic Redox Systems (GATES), with Generalized Electron Balance (GEB) concept involved in the GATES/GEB software. The results obtained from calculations made with use of iterative computer programs prepared according to MATLAB computational software, are presented graphically, as 2D and 3D graphs

  12. Polyarene mediators for mediated redox flow battery

    Energy Technology Data Exchange (ETDEWEB)

    Delnick, Frank M.; Ingersoll, David; Liang, Chengdu

    2018-01-02

    The fundamental charge storage mechanisms in a number of currently studied high energy redox couples are based on intercalation, conversion, or displacement reactions. With exception to certain metal-air chemistries, most often the active redox materials are stored physically in the electrochemical cell stack thereby lowering the practical gravimetric and volumetric energy density as a tradeoff to achieve reasonable power density. In a general embodiment, a mediated redox flow battery includes a series of secondary organic molecules that form highly reduced anionic radicals as reaction mediator pairs for the reduction and oxidation of primary high capacity redox species ex situ from the electrochemical cell stack. Arenes are reduced to stable anionic radicals that in turn reduce a primary anode to the charged state. The primary anode is then discharged using a second lower potential (more positive) arene. Compatible separators and solvents are also disclosed herein.

  13. Redox- and non-redox-metal-induced formation of free radicals and their role in human disease.

    Science.gov (United States)

    Valko, Marian; Jomova, Klaudia; Rhodes, Christopher J; Kuča, Kamil; Musílek, Kamil

    2016-01-01

    Transition metal ions are key elements of various biological processes ranging from oxygen formation to hypoxia sensing, and therefore, their homeostasis is maintained within strict limits through tightly regulated mechanisms of uptake, storage and secretion. The breakdown of metal ion homeostasis can lead to an uncontrolled formation of reactive oxygen species, ROS (via the Fenton reaction, which produces hydroxyl radicals), and reactive nitrogen species, RNS, which may cause oxidative damage to biological macromolecules such as DNA, proteins and lipids. An imbalance between the formation of free radicals and their elimination by antioxidant defense systems is termed oxidative stress. Most vulnerable to free radical attack is the cell membrane which may undergo enhanced lipid peroxidation, finally producing mutagenic and carcinogenic malondialdehyde and 4-hydroxynonenal and other exocyclic DNA adducts. While redox-active iron (Fe) and copper (Cu) undergo redox-cycling reactions, for a second group of redox-inactive metals such as arsenic (As) and cadmium (Cd), the primary route for their toxicity is depletion of glutathione and bonding to sulfhydryl groups of proteins. While arsenic is known to bind directly to critical thiols, other mechanisms, involving formation of hydrogen peroxide under physiological conditions, have been proposed. Redox-inert zinc (Zn) is the most abundant metal in the brain and an essential component of numerous proteins involved in biological defense mechanisms against oxidative stress. The depletion of zinc may enhance DNA damage by impairing DNA repair mechanisms. Intoxication of an organism by arsenic and cadmium may lead to metabolic disturbances of redox-active copper and iron, with the occurrence of oxidative stress induced by the enhanced formation of ROS/RNS. Oxidative stress occurs when excessive formation of ROS overwhelms the antioxidant defense system, as is maintained by antioxidants such as ascorbic acid, alpha

  14. The influence of operational and design parameters on vertical redox profiles in sub-surface flow constructed wetlands: surveying the optimal scenario for microbial fuel cell implementation

    OpenAIRE

    Garfi, Marianna; Corbella Vidal, Clara; Puigagut Juárez, Jaume

    2013-01-01

    The objective of the present work was to determine the optimal redox gradient that can be obtained in sub-surface flow constructed wetlands (SSF CWs) to maximize the energy production with microbial fuel cells (MFCs). To this aim, a pilot plant based on SSF CW was evaluated for vertical redox profiles. Key operational and design parameters surveyed that influences redox conditions in SSF CW were the presence of plants (Phragmites australis) and the flow regime (continuous and discontinuous fl...

  15. Modeling fluctuations in scattered waves

    CERN Document Server

    Jakeman, E

    2006-01-01

    Fluctuations in scattered waves limit the performance of imaging and remote sensing systems that operate on all wavelengths of the electromagnetic spectrum. To better understand these fluctuations, Modeling Fluctuations in Scattered Waves provides a practical guide to the phenomenology, mathematics, and simulation of non-Gaussian noise models and discusses how they can be used to characterize the statistics of scattered waves.Through their discussion of mathematical models, the authors demonstrate the development of new sensing techniques as well as offer intelligent choices that can be made for system analysis. Using experimental results and numerical simulation, the book illustrates the properties and applications of these models. The first two chapters introduce statistical tools and the properties of Gaussian noise, including results on phase statistics. The following chapters describe Gaussian processes and the random walk model, address multiple scattering effects and propagation through an extended med...

  16. Fluctuations in the multiparticle dynamics

    International Nuclear Information System (INIS)

    Bozek, P.; Ploszajczak, M.

    1993-01-01

    The appearance and properties of intermittent fluctuations in physical systems, in particular the formation of rare structures in transport phenomena are discussed. The distribution of fluctuations approaches a limiting log-normal statistical distribution. The log-normal distribution is introduced as a simple parametrization of the energy fluctuations leading to the subthreshold production of particles in nuclear collisions, and it is shown that it fits all available data both for total π 0 production cross section as well as the π 0 kinetic energy spectra for E/A < 90 MeV. It is suggested that the same universal distribution should also describe the subthreshold production of other hadrons like η and K. (author) 36 refs., 11 figs

  17. Cysteine-based redox regulation and signalling in plants

    Directory of Open Access Journals (Sweden)

    Jérémy eCouturier

    2013-04-01

    Full Text Available Living organisms are subjected to oxidative stress conditions which are characterized by the production of reactive oxygen (ROS, nitrogen (RNS and sulfur (RSS species. In plants as in other organisms, many of these compounds have a dual function as they damage different types of macromolecules but they also likely fulfil an important role as secondary messengers. Owing to the reactivity of their thiol groups, some protein cysteine residues are particularly prone to oxidation by these molecules. In the past years, besides their recognized catalytic and regulatory functions, the modification of cysteine thiol group was increasingly viewed as either protective or redox signalling mechanisms. The most physiologically relevant reversible redox post-translational modifications (PTMs are disulfide bonds, sulfenic acids, S-glutathionylated adducts, S-nitrosothiols and to a lesser extent S-sulfenylamides, thiosulfinates and S-persulfides. These redox PTMs are mostly controlled by two oxidoreductase families, thioredoxins and glutaredoxins. This review focuses on recent advances highlighting the variety and physiological roles of these PTMs and the proteomic strategies used for their detection.

  18. Fluctuation of Information Entropy Measures in Cell Image

    Directory of Open Access Journals (Sweden)

    Ishay Wohl

    2017-10-01

    Full Text Available A simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE which is shown to reflect intracellular biophysical properties like generalized entropy. In this study, the analytical relations between cellular thermodynamic generalized entropy and diffusivity and GLIE fluctuation measures are explored for the first time. The standard deviation (SD of GLIE is shown by experiments, simulation and theoretical analysis to be indifferent to microscope system “noise”. Then, the ability of GLIE fluctuation measures to reflect basic cellular entropy conditions of early death and malignancy is demonstrated in a cell model of human, healthy-donor lymphocytes, malignant Jurkat cells, as well as dead lymphocytes and Jurkat cells. Utilization of GLIE-based fluctuation measures seems to have the advantage of displaying biophysical characterization of the tested cells, like diffusivity and entropy, in a novel, unique, simple and illustrative way.

  19. Speciation of selenium in groundwater: Seasonal variations and redox transformations

    International Nuclear Information System (INIS)

    Kumar, A. Ramesh; Riyazuddin, P.

    2011-01-01

    Highlights: → Selenium(VI) was the predominant species of Se present in groundwater. → Groundwater recharge increased Se mobilization. → Dissolved oxygen and redox potential control the mobilization of soil selenium. → Shallow groundwater is susceptible for more selenium enrichment than deeper ones. - Abstract: Speciation of selenium in groundwater is essential from the viewpoint of toxicity to organisms and biogeochemical cycling. Selenium speciation in groundwater is controlled by aquifer redox conditions, microbial transformations, dissolved oxygen (DO) and other redox couples. A suburban area of Chennai city in India, where improper waste disposal measures have been practiced is selected for this study. Se(IV), Se(VI) and other hydrochemical parameters were monitored in shallow ground water during pre- and post-monsoon seasons for a period of three years. The objective of the study was to investigate the effect of groundwater recharge on selenium speciation. The concentration of Se(IV), and Se(VI) ranged between 0.15-0.43 μg L -1 and 0.16-4.73 μg L -1 , respectively. During post-monsoon period the concentration of Se(IV), and Se(VI) ranged between 0.15-1.25 μg L -1 and 0.58-10.37 μg L -1 , respectively. Se(VI) was the dominant species of selenium during the pre- and post-monsoon periods. During the post-monsoon periods, leaching of selenium from soil was more effective due to the increased oxidizing nature of the groundwater as indicated by the DO and redox potential (Eh) measurements. This finding has important implications on the behavior of selenium in groundwater, and also on the health of people consuming groundwater from seleniferous areas.

  20. Tracing iron-carbon redox from surface to core

    Science.gov (United States)

    McCammon, C. A.; Cerantola, V.; Bykova, E.; Kupenko, I.; Bykov, M.; Chumakov, A. I.; Rüffer, R.; Dubrovinsky, L. S.

    2017-12-01

    Numerous redox reactions separate the Earth's oxidised surface from its reduced core. Many involve iron, the Earth's most abundant element and the mantle's most abundant transition element. Most iron redox reactions (although not all) also involve other elements, including carbon, where iron-carbon interactions drive a number of important processes within the Earth, for example diamond formation. Many of the Earth's redox boundaries are sharp, much like the seismic properties that define them, for example between the lower mantle and the core. Other regions that appear seismically homogeneous, for example the lower mantle, harbour a wealth of reactions between oxidised and reduced phases of iron and carbon. We have undertaken many experiments at high pressure and high temperature on phases containing iron and carbon using synchrotron-based X-rays to probe structures and iron oxidation states. Results demonstrate the dominant role that crystal structures play in determining the stable oxidation states of iron and carbon, even when oxygen fugacity (and common sense) would suggest otherwise. Iron in bridgmanite, for example, occurs predominantly in its oxidised form (ferric iron) throughout the lower mantle, despite the inferred reducing conditions. Newly discovered structures of iron carbonate also stabilise ferric iron, while simultaneously reducing some carbon to diamond to balance charge. Other high-pressure iron carbonates appear to be associated with the emerging zoo of iron oxide phases, involving transitions between ferrous and ferric iron through the exchange of oxygen. The presentation will trace redox relations between iron and carbon from the Earth's surface to its core, with an emphasis on recent experimental results.

  1. The redox status of cystinotic fibroblasts.

    Science.gov (United States)

    Vitvitsky, Victor; Witcher, Marc; Banerjee, Ruma; Thoene, Jess

    2010-04-01

    A key unresolved question in the pathogenesis of phenotype development in nephropathic cystinosis is whether intralysosomal cystine, the hallmark of this lethal inborn error of metabolism, alters cytoplasmic redox potential. Variable findings on this issue have been reported. This study of fetal and non-fetal skin and lung-derived cystinotic fibroblasts compared to origin and age-matched normal control fibroblasts reveals that cystinotic cells do not exhibit redox perturbations. We find that the steady-state redox status as assessed by the [GSH]/[GSSG] ratio, an indicator of the intracellular redox poise, is unchanged in cystinotic cells. Furthermore, the dependence of the intracellular GSH and cysteine pool sizes and the [GSH]/[GSSG] ratio are similarly dependent on the two major sources of cysteine, i.e. the transsulfuration pathway and the plasma membrane cystine transporter, xc(-), in both cystinotic and control cells, and the presence of lysosomal cystine has no measurable effect on the redox status of these cells. Hence, mechanisms other than cytosolic redox perturbations are involved in the etiology of nephropathic cystinosis. Copyright 2009 Elsevier Inc. All rights reserved.

  2. Membranes for Redox Flow Battery Applications

    Science.gov (United States)

    Prifti, Helen; Parasuraman, Aishwarya; Winardi, Suminto; Lim, Tuti Mariana; Skyllas-Kazacos, Maria

    2012-01-01

    The need for large scale energy storage has become a priority to integrate renewable energy sources into the electricity grid. Redox flow batteries are considered the best option to store electricity from medium to large scale applications. However, the current high cost of redox flow batteries impedes the wide spread adoption of this technology. The membrane is a critical component of redox flow batteries as it determines the performance as well as the economic viability of the batteries. The membrane acts as a separator to prevent cross-mixing of the positive and negative electrolytes, while still allowing the transport of ions to complete the circuit during the passage of current. An ideal membrane should have high ionic conductivity, low water intake and excellent chemical and thermal stability as well as good ionic exchange capacity. Developing a low cost, chemically stable membrane for redox flow cell batteries has been a major focus for many groups around the world in recent years. This paper reviews the research work on membranes for redox flow batteries, in particular for the all-vanadium redox flow battery which has received the most attention. PMID:24958177

  3. Membranes for Redox Flow Battery Applications

    Directory of Open Access Journals (Sweden)

    Maria Skyllas-Kazacos

    2012-06-01

    Full Text Available The need for large scale energy storage has become a priority to integrate renewable energy sources into the electricity grid. Redox flow batteries are considered the best option to store electricity from medium to large scale applications. However, the current high cost of redox flow batteries impedes the wide spread adoption of this technology. The membrane is a critical component of redox flow batteries as it determines the performance as well as the economic viability of the batteries. The membrane acts as a separator to prevent cross-mixing of the positive and negative electrolytes, while still allowing the transport of ions to complete the circuit during the passage of current. An ideal membrane should have high ionic conductivity, low water intake and excellent chemical and thermal stability as well as good ionic exchange capacity. Developing a low cost, chemically stable membrane for redox flow cell batteries has been a major focus for many groups around the world in recent years. This paper reviews the research work on membranes for redox flow batteries, in particular for the all-vanadium redox flow battery which has received the most attention.

  4. Origin of cosmological density fluctuations

    International Nuclear Information System (INIS)

    Carr, B.J.

    1984-11-01

    The density fluctuations required to explain the large-scale cosmological structure may have arisen spontaneously as a result of a phase transition in the early Universe. There are several ways in which such fluctuations may have ben produced, and they could have a variety of spectra, so one should not necessarily expect all features of the large-scale structure to derive from a simple power law spectrum. Some features may even result from astrophysical amplification mechanisms rather than gravitational instability. 128 references

  5. Adaptations to endurance training depend on exercise-induced oxidative stress: exploiting redox interindividual variability.

    Science.gov (United States)

    Margaritelis, N V; Theodorou, A A; Paschalis, V; Veskoukis, A S; Dipla, K; Zafeiridis, A; Panayiotou, G; Vrabas, I S; Kyparos, A; Nikolaidis, M G

    2018-02-01

    The aim of this study was to reveal the role of reactive oxygen and nitrogen species (RONS) in exercise adaptations under physiological in vivo conditions and without the interference from other exogenous redox agents (e.g. a pro-oxidant or antioxidant). We invented a novel methodological set-up that exploited the large redox interindividual variability in exercise responses. More specifically, we used exercise-induced oxidative stress as the 'classifier' measure (i.e. low, moderate and high) and investigated the physiological and redox adaptations after a 6-week endurance training protocol. We demonstrated that the group with the low exercise-induced oxidative stress exhibited the lowest improvements in a battery of classic adaptations to endurance training (VO 2 max, time trial and Wingate test) as well as in a set of redox biomarkers (oxidative stress biomarkers and antioxidants), compared to the high and moderate oxidative stress groups. The findings of this study substantiate, for the first time in a human in vivo physiological context, and in the absence of any exogenous redox manipulation, the vital role of RONS produced during exercise in adaptations. The stratification approach, based on a redox phenotype, implemented in this study could be a useful experimental strategy to reveal the role of RONS and antioxidants in other biological manifestations as well. © 2017 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.

  6. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators.

    Science.gov (United States)

    Dooley, Colette T; Dore, Timothy M; Hanson, George T; Jackson, W Coyt; Remington, S James; Tsien, Roger Y

    2004-05-21

    Changes in the redox equilibrium of cells influence a host of cell functions. Alterations in the redox equilibrium are precipitated by changing either the glutathione/glutathione-disulfide ratio (GSH/GSSG) and/or the reduced/oxidized thioredoxin ratio. Redox-sensitive green fluorescent proteins (GFP) allow real time visualization of the oxidation state of the indicator. Ratios of fluorescence from excitation at 400 and 490 nm indicate the extent of oxidation and thus the redox potential while canceling out the amount of indicator and the absolute optical sensitivity. Because the indicator is genetically encoded, it can be targeted to specific proteins or organelles of interest and expressed in a wide variety of cells and organisms. We evaluated roGFP1 (GFP with mutations C48S, S147C, and Q204C) and roGFP2 (the same plus S65T) with physiologically or toxicologically relevant oxidants both in vitro and in living mammalian cells. Furthermore, we investigated the response of the redox probes under physiological redox changes during superoxide bursts in macrophage cells, hyperoxic and hypoxic conditions, and in responses to H(2)O(2)-stimulating agents, e.g. epidermal growth factor and lysophosphatidic acid.

  7. STIR: Redox-Switchable Olefin Polymerization Catalysis: Electronically Tunable Ligands for Controlled Polymer Synthesis

    Science.gov (United States)

    2013-03-28

    were reduced using standard hydrogenation conditions with hydrogen and palladium on carbon . Though the synthesis of 1,1’-diaminoferrocene has...forward reduction of diimine 7 proved unusually difficult under typical conditions, although a stepwise procedure utilizing formic acid and sodium...REPORT Final Report: STIR: Redox-Switchable Olefin Polymerization Catalysis: Electronically Tunable Ligands for Controlled Polymer Synthesis 14

  8. Redox Nano-Architectures: Perspectives and Implications in Diagnosis and Treatment of Human Diseases.

    Science.gov (United States)

    Sufi, Shamim Akhtar; Pajaniradje, Sankar; Mukherjee, Victor; Rajagopalan, Rukkumani

    2018-03-02

    Efficient targeted therapy with minimal side-effects is the need of the hour. Locally altered redox state is observed in several human ailments, such as inflammation, sepsis, and cancer. This has been taken advantage of in designing redox-responsive nanodrug carriers. Redox-responsive nanosystems open a door to a multitude of possibilities for the control of diseases over other drug delivery systems. Recent Advances: The first-generation nanotherapy relies on novel properties of nanomaterials to shield the drug and deliver it to the diseased tissue or organ. The second generation is based on targeting the drug or diagnostic material to the diseased cell-specific receptors, or to a particular organ to improve the efficacy of the drug. The third and the latest generation of nanocarriers, the stimuli-responsive nanocarriers exploit the disease condition or environment to specifically deliver the drug or diagnostic probe for the best diagnosis and treatment. Several different kinds of stimuli such as temperature, magnetic field, pH, and altered redox state-responsive nanosystems have educed immense promise in the field of nanomedicine and therapy. We describe the evolution of nanomaterial since its inception with an emphasis on stimuli-responsive nanocarriers, especially redox-sensitive nanocarriers. Importantly, we discuss the future perspectives of redox-responsive nanocarriers and their implications. Redox-responsive nanocarriers achieve a near-to-zero premature release of the drug, thus avoiding off-site toxicity associated with the free drug. This bears great potential for the development of more effective drug delivery with better pharmacokinetics and pharmacodynamics. Antioxid. Redox Signal. 00, 000-000.

  9. Redox-active labile iron in fortified flours from the Brazilian market Ferro lábil redox-ativo em farinhas fortificadas do mercado brasileiro

    Directory of Open Access Journals (Sweden)

    Breno Pannia Espósito

    2007-08-01

    Full Text Available OBJECTIVE: To quantify the fraction of redox-active labile iron in iron-fortified flours acquired on the Brazilian market. METHODS: Samples of wheat flour, maize flour and breadcrumbs were extracted with buffers that mimic gastric juice, saliva and intestinal juice. Redox-active labile iron levels were assessed through the reaction of autoxidation of ascorbic acid catalyzed by iron in the presence of a fluorescence probe. RESULTS: Redox-active labile iron represents 1% to 9% of the total iron in the flour and breadcrumb samples, with the lowest values found under gastric juice conditions and the highest in the more alkaline media. Redox-active labile iron possibly arises from the decomposition of an iron-phytic acid complex. A positive correlation between redox-active labile iron and total iron was found in saline biomimetic fluids. CONCLUSION: Redox-active labile iron may be a risk factor for people with impaired antioxidant defenses, such as those who are atransferrinemic or iron overloaded (e.g. thalassemic. Total iron can be used to predict redox-active labile iron absorption at each stage of the gastrointestinal tract after ingestion of iron-fortified flours.OBJETIVO: Quantificar a porcentagem de ferro lábil redox ativo em farinhas fortificadas adquiridas no comércio popular. MÉTODOS: Amostras de farinha de trigo, fubá e rosca foram extraídas com tampões miméticos de suco gástrico, saliva e suco intestinal. Os níveis de ferro lábil redox ativo foram determinados por meio da reação de auto-oxidação do ácido ascórbico catalisada pelo ferro, em presença de uma sonda fluorimétrica. RESULTADOS: A fração de ferro lábil redox ativo representa entre 1% e 9% do ferro total nas farinhas estudadas, sendo os menores valores encontrados em condições miméticas do suco gástrico e os maiores nos meios mais alcalinos. Há indícios de que o ferro lábil redox ativo origina-se da decomposição de um complexo entre ferro e ácido f

  10. Universal fluctuations in orbital diamagnetism

    Science.gov (United States)

    Pal, P. S.; Saha, Arnab; Jayannavar, A. M.

    2018-03-01

    Bohr-van Leuween theorem has attracted the notice of physicists for more than 100 years. The theorem states about the absence of magnetisation in classical systems in thermal equilibrium. In this paper, we discuss about fluctuations of magnetic moment in classical systems. In recent years, this topic has been investigated intensively and it is not free from controversy. We have considered a system consisting of a single particle moving in a plane. A magnetic field is applied perpendicular to the plane. The system is in contact with a thermal bath. We have considered three cases: (a) particle moving in a homogeneous medium, (b) particle moving in a medium with space-dependent friction and (c) particle moving in a medium with space-dependent temperature. For all the three cases, the average magnetic moment and fluctuations in magnetic moment have been calculated. Average magnetic moment saturates to a finite value in the case of free particle but goes to zero when the particle is confined by a 2D harmonic potential. Fluctuations in magnetic moment shows universal features in the presence of arbitrary friction inhomogeneity. For this case, the system reaches equilibrium asymptotically. In the case of space-dependent temperature profile, the stationary distribution is non-Gibbsian and fluctuations deviate from universal value for the bounded system only.

  11. Universal fluctuations in orbital diamagnetism

    Indian Academy of Sciences (India)

    P S Pal

    2018-01-31

    Jan 31, 2018 ... Indian Academy of Sciences https://doi.org/10.1007/s12043-018-1521-5. Universal fluctuations ... dissertation almost a century ago. They had shown that in the presence of constant magnetic field ..... Ph.D. Thesis (Indian Institute of Science, Bengaluru,. 1982). [7] N Kumar and K Vijay Kumar, Europhys. Lett.

  12. Fluctuating hydrodynamics for ionic liquids

    Energy Technology Data Exchange (ETDEWEB)

    Lazaridis, Konstantinos [Department of Mathematics and Statistics, Washington State University, Pullman, 99163 (United States); Wickham, Logan [Department of Computer Science, Washington State University, Richland, 99354 (United States); Voulgarakis, Nikolaos, E-mail: n.voulgarakis@wsu.edu [Department of Mathematics and Statistics, Washington State University, Pullman, 99163 (United States)

    2017-04-25

    We present a mean-field fluctuating hydrodynamics (FHD) method for studying the structural and transport properties of ionic liquids in bulk and near electrified surfaces. The free energy of the system consists of two competing terms: (1) a Landau–Lifshitz functional that models the spontaneous separation of the ionic groups, and (2) the standard mean-field electrostatic interaction between the ions in the liquid. The numerical approach used to solve the resulting FHD-Poisson equations is very efficient and models thermal fluctuations with remarkable accuracy. Such density fluctuations are sufficiently strong to excite the experimentally observed spontaneous formation of liquid nano-domains. Statistical analysis of our simulations provides quantitative information about the properties of ionic liquids, such as the mixing quality, stability, and the size of the nano-domains. Our model, thus, can be adequately parameterized by directly comparing our prediction with experimental measurements and all-atom simulations. Conclusively, this work can serve as a practical mathematical tool for testing various theories and designing more efficient mixtures of ionic liquids. - Highlights: • A new fluctuating hydrodynamics method for ionic liquids. • Description of ionic liquid morphology in bulk and near electrified surfaces. • Direct comparison with experimental measurements.

  13. Fluctuation relation for heat engines

    International Nuclear Information System (INIS)

    Sinitsyn, N A

    2011-01-01

    We derive the exact equality, referred to as the fluctuation relation for heat engines (FRHE), that relates statistics of heat extracted from one of the two heat baths and the work per one cycle of a heat engine operation. Carnot's inequality of classical thermodynamics follows as a direct consequence of the FRHE. (paper)

  14. Reaction rates when barriers fluctuate

    OpenAIRE

    Reimann, Peter

    1999-01-01

    Reaction rates when barriers fluctuate : a path integral approach / P. Hänggi and P. Reimann. - In: International Conference on Path Integrals from peV to TeV : Proceedings of the ... / eds.: R. Casalbuoni ... - Singapore u.a. : World Scientific, 1999. - S. 407-409

  15. Fluctuation conductivity in cuprate superconductors

    Indian Academy of Sciences (India)

    model to be inadequate to describe the fluctuation conductivity in these materials. The modification ... shown by various models which consider several conducting layers per unit cell, with ei- ther interlayer or ..... Pomer et al [6] have observed a large discrepancy of their data measured at 1 tesla from the prediction of eq. (1).

  16. Kondo effect and mesoscopic fluctuations

    Indian Academy of Sciences (India)

    Slave boson/fermion mean-field approach. A complete solution of this problem would presumably involve developing a Fermi liquid theory 'à la Nozières' [15] taking properly into account the mesoscopic fluctuations. A first step in this direction is to use a mean-field treatment based on the slave boson/fermion technique [1] ...

  17. Funnel's Fluctuations in Dyonic Case: Intersecting D1-D3 Branes

    Directory of Open Access Journals (Sweden)

    Douari J.

    2007-07-01

    Full Text Available The fluctuations of funnel solutions of intersecting D1 and D3 branes are quite explicitly discussed by treating different modes and different directions of the fluctuation at the presence of world volume electric field. The boundary conditions are found to be Neumann boundary conditions.

  18. Redox interplay between mitochondria and peroxisomes

    Directory of Open Access Journals (Sweden)

    Celien eLismont

    2015-05-01

    Full Text Available Reduction-oxidation or ‘redox’ reactions are an integral part of a broad range of cellular processes such as gene expression, energy metabolism, protein import and folding, and autophagy. As many of these processes are intimately linked with cell fate decisions, transient or chronic changes in cellular redox equilibrium are likely to contribute to the initiation and progression of a plethora of human diseases. Since a long time, it is known that mitochondria are major players in redox regulation and signaling. More recently, it has become clear that also peroxisomes have the capacity to impact redox-linked physiological processes. To serve this function, peroxisomes cooperate with other organelles, including mitochondria. This review provides a comprehensive picture of what is currently known about the redox interplay between mitochondria and peroxisomes in mammals. We first outline the pro- and antioxidant systems of both organelles and how they may function as redox signaling nodes. Next, we critically review and discuss emerging evidence that peroxisomes and mitochondria share an intricate redox-sensitive relationship and cooperate in cell fate decisions. Key issues include possible physiological roles, messengers, and mechanisms. We also provide examples of how data mining of publicly-available datasets from ‘omics’ technologies can be a powerful means to gain additional insights into potential redox signaling pathways between peroxisomes and mitochondria. Finally, we highlight the need for more studies that seek to clarify the mechanisms of how mitochondria may act as dynamic receivers, integrators, and transmitters of peroxisome-derived mediators of oxidative stress. The outcome of such studies may open up exciting new avenues for the community of researchers working on cellular responses to organelle-derived oxidative stress, a research field in which the role of peroxisomes is currently highly underestimated and an issue of

  19. Single-molecule conductance of redox molecules in electrochemical scanning tunneling microscopy

    DEFF Research Database (Denmark)

    Haiss, W.; Albrecht, Tim; van Zalinge, H.

    2007-01-01

    across the molecular bridge through control of the electrochemical potential and consequently the redox state of the viologen moiety. This gating is theoretically considered within the framework of superexchange and coherent two-step notions for charge transport. It is shown here that the absence...... of a maximum in the I-tunneling versus electrode potential relationship can be fitted by a "soft" gating concept. This arises from large configurational fluctuations of the molecular bridge linked to the gold contacts by flexible chains. This view is incorporated in a formalism that is well-suited for data...

  20. Transitions in optimal adaptive strategies for populations in fluctuating environments

    Science.gov (United States)

    Mayer, Andreas; Mora, Thierry; Rivoire, Olivier; Walczak, Aleksandra M.

    2017-09-01

    Biological populations are subject to fluctuating environmental conditions. Different adaptive strategies can allow them to cope with these fluctuations: specialization to one particular environmental condition, adoption of a generalist phenotype that compromises between conditions, or population-wise diversification (bet hedging). Which strategy provides the largest selective advantage in the long run depends on the range of accessible phenotypes and the statistics of the environmental fluctuations. Here, we analyze this problem in a simple mathematical model of population growth. First, we review and extend a graphical method to identify the nature of the optimal strategy when the environmental fluctuations are uncorrelated. Temporal correlations in environmental fluctuations open up new strategies that rely on memory but are mathematically challenging to study: We present analytical results to address this challenge. We illustrate our general approach by analyzing optimal adaptive strategies in the presence of trade-offs that constrain the range of accessible phenotypes. Our results extend several previous studies and have applications to a variety of biological phenomena, from antibiotic resistance in bacteria to immune responses in vertebrates.

  1. Predicting redox conditions in groundwater at a regional scale

    Science.gov (United States)

    Tesoriero, Anthony J.; Terziotti, Silvia; Abrams, Daniel B.

    2015-01-01

    Defining the oxic-suboxic interface is often critical for determining pathways for nitrate transport in groundwater and to streams at the local scale. Defining this interface on a regional scale is complicated by the spatial variability of reaction rates. The probability of oxic groundwater in the Chesapeake Bay watershed was predicted by relating dissolved O2 concentrations in groundwater samples to indicators of residence time and/or electron donor availability using logistic regression. Variables that describe surficial geology, position in the flow system, and soil drainage were important predictors of oxic water. The probability of encountering oxic groundwater at a 30 m depth and the depth to the bottom of the oxic layer were predicted for the Chesapeake Bay watershed. The influence of depth to the bottom of the oxic layer on stream nitrate concentrations and time lags (i.e., time period between land application of nitrogen and its effect on streams) are illustrated using model simulations for hypothetical basins. Regional maps of the probability of oxic groundwater should prove useful as indicators of groundwater susceptibility and stream susceptibility to contaminant sources derived from groundwater.

  2. Behaviour of radionuclides in sedimentation processes under varying redox conditions

    Energy Technology Data Exchange (ETDEWEB)

    Ilus, E.; Ikaeheimonen, T.K.; Mattila, J.; Klemola, S. [STUK Radiation and Nuclear Safety Authority (Finland)

    2001-04-01

    Determination of sedimentation rates plays an important role in material balance and model calculations of seas and other bodies of water. The Baltic Sea offers an exceptionally good opportunity to study processes in sediments and sedimentation rates with radioecological methods, because the concentration peaks of {sup 137}Cs and {sup 239,240}Pu are easily detectable in its sediments. In 1995-1996 sediment profiles were taken at 51 sampling stations situated in the Baltic Proper, Bothnian Bay, Bothnian Sea and Gulf of Finland. The aim was to estimate sedimentation rates in different parts of the Baltic Sea by using alternative methods and to consider reasons for eventual differences in results. The {sup 210}Pb, {sup 137}Cs, {sup 239,240}Pu and th sediment trap methods were used in estimations. The results show that the accumulation rates of dry matter may vary between 0.006 and 0.90 g cm{sup -2}y{sup -1} at different sampling stations of the Baltic Sea and the sedimentation rates between 0.2 and 29 mm y{sup -1} depending on the sedimentation itself and the method used in calculation. This is a considerable range in results, considering that all of the sampling stations were located in areas of soft sediment bottoms. In general, the sedimentation rates were highest at the Bothnian Sea sampling stations. In the Gulf of Finland the sedimentation rates were highest in the eastern part, while in the Bothnian Bay and in the Baltic Proper the rates were in general lower than in the 2 areas first mentioned. The differences among the results obtained with various methods varied unsystematically; thus it was not possible to predict that anyone of the methods would always give higher results than any of the others or vice versa. The results show that in the Baltic Sea the use of more than 1 parallel methods in estimation of sedimentation rate is highly recommended. None of the methods is necessarily suitable for routine use in the Baltic Sea. In those cases where the {sup 137}Cs or {sup 239,240}Pu peak is distinct, sharply defined and at sufficient depth, these methods may result in the best estimates. One the other hand, at those stations where the peaks are spread out over a broad range of depths, the methods based on {sup 210}Pb may give more accurate results. On of the advantages of the {sup 210}Pb-based methods is that they give information on sedimentation rate over longer period of time than the {sup 137}Cs and {sup 239,240}Pu methods. In contrast to the {sup 239,240}Pu method, {sup 210}Pb can be analysed gammaspectrometrically; however the gammaspectrometric method for {sup 210}Pb is very demanding both for the equipment and the staff. At least in the case of our material, the practice used in the CF:CS-model appeared to be the most useable {sup 210}Pb-methods for the Baltic Sea sediments. (EHS)

  3. Hydrogen as indicator for redox conditions and dechlorination

    NARCIS (Netherlands)

    Meer, J. ter; Gerritse, J.; Mauro, C. di; Harkes, M.P.; Rijnaarts, H.H.M.

    2000-01-01

    The use of intrinsic degradation processes (natural attenuation) is becoming the basis for the restoration of many contaminated sites. Within this context, measuring concentrations of hydrogen in groundwater is important in two ways. Firstly, the hydrogen concentration can be used to characterize

  4. Controlled Chemical Doping of Semiconductor Nanocrystals Using Redox Buffers

    Energy Technology Data Exchange (ETDEWEB)

    Engel, Jesse H. [Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Surendranath, Yogesh [Univ. of California, Berkeley, CA (United States); Alivisatos, Paul [Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

    2013-07-20

    Semiconductor nanocrystal solids are attractive materials for active layers in next-generation optoelectronic devices; however, their efficient implementation has been impeded by the lack of precise control over dopant concentrations. Herein we demonstrate a chemical strategy for the controlled doping of nanocrystal solids under equilibrium conditions. Exposing lead selenide nanocrystal thin films to solutions containing varying proportions of decamethylferrocene and decamethylferrocenium incrementally and reversibly increased the carrier concentration in the solid by 2 orders of magnitude from their native values. This application of redox buffers for controlled doping provides a new method for the precise control of the majority carrier concentration in porous semiconductor thin films.

  5. Colloid mobilization and transport during capillary fringe fluctuations.

    Science.gov (United States)

    Aramrak, Surachet; Flury, Markus; Harsh, James B; Zollars, Richard L

    2014-07-01

    Capillary fringe fluctuations due to changing water tables lead to displacement of air-water interfaces in soils and sediments. These moving air-water interfaces can mobilize colloids. We visualized colloids interacting with moving air-water interfaces during capillary fringe fluctuations by confocal microscopy. We simulated capillary fringe fluctuations in a glass-bead-filled column. We studied four specific conditions: (1) colloids suspended in the aqueous phase, (2) colloids attached to the glass beads in an initially wet porous medium, (3) colloids attached to the glass beads in an initially dry porous medium, and (4) colloids suspended in the aqueous phase with the presence of a static air bubble. Confocal images confirmed that the capillary fringe fluctuations affect colloid transport behavior. Hydrophilic negatively charged colloids initially suspended in the aqueous phase were deposited at the solid-water interface after a drainage passage, but then were removed by subsequent capillary fringe fluctuations. The colloids that were initially attached to the wet or dry glass bead surface were detached by moving air-water interfaces in the capillary fringe. Hydrophilic negatively charged colloids did not attach to static air-bubbles, but hydrophobic negatively charged and hydrophilic positively charged colloids did. Our results demonstrate that capillary fringe fluctuations are an effective means for colloid mobilization.

  6. De Novo Construction of Redox Active Proteins.

    Science.gov (United States)

    Moser, C C; Sheehan, M M; Ennist, N M; Kodali, G; Bialas, C; Englander, M T; Discher, B M; Dutton, P L

    2016-01-01

    Relatively simple principles can be used to plan and construct de novo proteins that bind redox cofactors and participate in a range of electron-transfer reactions analogous to those seen in natural oxidoreductase proteins. These designed redox proteins are called maquettes. Hydrophobic/hydrophilic binary patterning of heptad repeats of amino acids linked together in a single-chain self-assemble into 4-alpha-helix bundles. These bundles form a robust and adaptable frame for uncovering the default properties of protein embedded cofactors independent of the complexities introduced by generations of natural selection and allow us to better understand what factors can be exploited by man or nature to manipulate the physical chemical properties of these cofactors. Anchoring of redox cofactors such as hemes, light active tetrapyrroles, FeS clusters, and flavins by His and Cys residues allow cofactors to be placed at positions in which electron-tunneling rates between cofactors within or between proteins can be predicted in advance. The modularity of heptad repeat designs facilitates the construction of electron-transfer chains and novel combinations of redox cofactors and new redox cofactor assisted functions. Developing de novo designs that can support cofactor incorporation upon expression in a cell is needed to support a synthetic biology advance that integrates with natural bioenergetic pathways. © 2016 Elsevier Inc. All rights reserved.

  7. Compartmentation of redox metabolism in malaria parasites.

    Directory of Open Access Journals (Sweden)

    Sebastian Kehr

    Full Text Available Malaria, caused by the apicomplexan parasite Plasmodium, still represents a major threat to human health and welfare and leads to about one million human deaths annually. Plasmodium is a rapidly multiplying unicellular organism undergoing a complex developmental cycle in man and mosquito - a life style that requires rapid adaptation to various environments. In order to deal with high fluxes of reactive oxygen species and maintain redox regulatory processes and pathogenicity, Plasmodium depends upon an adequate redox balance. By systematically studying the subcellular localization of the major antioxidant and redox regulatory proteins, we obtained the first complete map of redox compartmentation in Plasmodium falciparum. We demonstrate the targeting of two plasmodial peroxiredoxins and a putative glyoxalase system to the apicoplast, a non-photosynthetic plastid. We furthermore obtained a complete picture of the compartmentation of thioredoxin- and glutaredoxin-like proteins. Notably, for the two major antioxidant redox-enzymes--glutathione reductase and thioredoxin reductase--Plasmodium makes use of alternative-translation-initiation (ATI to achieve differential targeting. Dual localization of proteins effected by ATI is likely to occur also in other Apicomplexa and might open new avenues for therapeutic intervention.

  8. Structure, stabilité et fluctuations

    CERN Document Server

    Glansdorff, P

    1971-01-01

    Théorie générale : lois de conservation et bilans ; le second principe de la thermodynamique et le bilan entropique ; thermodynamique linéaire des phénomènes irréversibles ; théorie de la stabilité de l'équilibre thermodynamique par la méthode Gibbs-Duhem ; théorie générale de la stabilité de l'équilibre thermodynamique ; conditions de stabilité thermodynamique et hydrodynamique des syst¿mes hors d'équilibre ; forme explicite des conditions de stablité des processus hors d'équilibre ; stabilité et fluctuations ; le critère général d'évolution.

  9. Relationships Between Redox Potential and Sediment Organic Matter Characteristics and Consequences for Restoration of Aquatic Vegetation

    Science.gov (United States)

    Laing, J.

    2015-12-01

    Eutrophication in rivers is often characterized by sharp increases in filamentous algae and sediment organic matter and decreases in populations of native submerged aquatic vegetation (SAV). Because established SAV oxygenate the rhizosphere of underlying sediments, declines in SAV and increases in sediment organic matter may result in highly reduced conditions in river bottom sediments. These reduced sediments may contain phytotoxic compounds which inhibit the establishment and early growth of SAV. In this study we measured sediment redox potential in three subtropical spring-fed rivers. For each river we compared redox potential in sediments high in organic matter with redox potential in mineral sediments and in sediments underlying SAV beds (n=9). Additionally, we collected plant biomass and sediment samples to investigate relationships between sediment redox potential and its potential drivers. Preliminary results show that sediments underlying SAV beds high in belowground biomass had higher relative redox potential than unvegetated organic and mineral sediments. These results have strong implications for SAV restoration plantings. Reducing conditions in unvegetated sites dominated by filamentous algae may cause widespread plant senescence when sediments are not properly prepared for planting.

  10. Redox behaviour of uranium with iron compounds

    International Nuclear Information System (INIS)

    Ithurbide, A.

    2009-10-01

    An option investigated for the management of long-term nuclear waste is a repository in deep geological formations. It is generally admitted that the release of radionuclides from the spent fuel in the geosphere could occur several thousand years after the beginning of the storage. Therefore, to assess the safety of the long-term disposal, it is important to consider the phenomena that can reduce the migration, and in particular the migration of uranium. The aim of this work is to study if siderite, an iron compound present both in the near - and far -field, can limit this migration as well as the role played by the redox process. Siderite thin layers have been obtained by electrochemistry. The layers are adherent and homogeneous. Their thickness is about 1 μm and they are composed of spherical grains. Analytical characterizations performed show that siderite is free of any impurity and does not exhibit any trace of oxidation. The interactions between siderite and uranium (VI) have been carried out in solutions considered as representative of environmental waters, in terms of pH and carbonate concentration. The retention of uranium on the thin layer is important since, after 24 hours of interaction, it corresponds to retention capacities of several hundreds of uranium micro-moles per gram of siderite. XPS analysis show that, in any studied condition, part of uranium present on the thin layer is reduced into an over stoichiometric uranium dioxide. The process of interaction differs depending on the considered environment, specially on the stability of siderite. (author)

  11. Photon counting and fluctuation of molecular movement

    International Nuclear Information System (INIS)

    Inohara, Koichi

    1978-01-01

    The direct measurement of the fluctuation of molecular motions, which provides with useful information on the molecular movement, was conducted by introducing photon counting method. The utilization of photon counting makes it possible to treat the molecular system consisting of a small number of molecules like a radioisotope in the detection of a small number of atoms, which are significant in biological systems. This method is based on counting the number of photons of the definite polarization emitted in a definite time interval from the fluorescent molecules excited by pulsed light, which are bound to the marked large molecules found in a definite spatial region. Using the probability of finding a number of molecules oriented in a definite direction in the definite spatial region, the probability of counting a number of photons in a definite time interval can be calculated. Thus the measurable count rate of photons can be related with the fluctuation of molecular movement. The measurement was carried out under the condition, in which the probability of the simultaneous arrival of more than two photons at a detector is less than 1/100. As the experimental results, the resolving power of photon-counting apparatus, the frequency distribution of the number of photons of some definite polarization counted for 1 nanosecond are shown. In the solution, the variance of the number of molecules of 500 on the average is 1200, which was estimated from the experimental data by assuming normal distribution. This departure from the Poisson distribution means that a certain correlation does exist in molecular movement. In solid solution, no significant deviation was observed. The correlation existing in molecular movement can be expressed in terms of the fluctuation of the number of molecules. (Nakai, Y.)

  12. Modeling seasonal redox dynamics and the corresponding fate of the pharmaceutical residue phenazone during artificial recharge of groundwater.

    Science.gov (United States)

    Greskowiak, Janek; Prommer, Henning; Massmann, Gudrun; Nützmann, Gunnar

    2006-11-01

    Reactive multicomponent transport modeling was used to investigate and quantify the factors that affect redox zonation and the fate of the pharmaceutical residue phenazone during artificial recharge of groundwater at an infiltration site in Berlin, Germany. The calibrated model and the corresponding sensitivity analysis demonstrated thattemporal and spatial redox zonation at the study site was driven by seasonally changing, temperature-dependent organic matter degradation rates. Breakthrough of phenazone at monitoring wells occurred primarily during the warmer summer months, when anaerobic conditions developed. Assuming a redox-sensitive phenazone degradation behavior the model results provided an excellent agreement between simulated and measured phenazone concentrations. Therefore, the fate of phenazone was shown to be indirectly controlled by the infiltration water temperature through its effect on the aquifer's redox conditions. Other factors such as variable residence times appeared to be of less importance.

  13. Fluctuation conductivity in cuprate superconductors

    Indian Academy of Sciences (India)

    Abstract. We have measured the in-plane resistivity of Bi2Sr2CaCu2O8+δ and Tl2Ba2. CaCu2O8+δ single crystals in the temperature range 70–300 K. The thermodynamic fluctuations in the conductivity of both the samples start around ∼ 125 K. We find the Lawrence and Doniach [1] model to be inadequate to describe the ...

  14. Random numbers from vacuum fluctuations

    International Nuclear Information System (INIS)

    Shi, Yicheng; Kurtsiefer, Christian; Chng, Brenda

    2016-01-01

    We implement a quantum random number generator based on a balanced homodyne measurement of vacuum fluctuations of the electromagnetic field. The digitized signal is directly processed with a fast randomness extraction scheme based on a linear feedback shift register. The random bit stream is continuously read in a computer at a rate of about 480 Mbit/s and passes an extended test suite for random numbers.

  15. Fluctuating hydrodynamics for ionic liquids

    Science.gov (United States)

    Lazaridis, Konstantinos; Wickham, Logan; Voulgarakis, Nikolaos

    2017-04-01

    We present a mean-field fluctuating hydrodynamics (FHD) method for studying the structural and transport properties of ionic liquids in bulk and near electrified surfaces. The free energy of the system consists of two competing terms: (1) a Landau-Lifshitz functional that models the spontaneous separation of the ionic groups, and (2) the standard mean-field electrostatic interaction between the ions in the liquid. The numerical approach used to solve the resulting FHD-Poisson equations is very efficient and models thermal fluctuations with remarkable accuracy. Such density fluctuations are sufficiently strong to excite the experimentally observed spontaneous formation of liquid nano-domains. Statistical analysis of our simulations provides quantitative information about the properties of ionic liquids, such as the mixing quality, stability, and the size of the nano-domains. Our model, thus, can be adequately parameterized by directly comparing our prediction with experimental measurements and all-atom simulations. Conclusively, this work can serve as a practical mathematical tool for testing various theories and designing more efficient mixtures of ionic liquids.

  16. Heat fluctuations and initial ensembles

    Science.gov (United States)

    Kim, Kwangmoo; Kwon, Chulan; Park, Hyunggyu

    2014-09-01

    Time-integrated quantities such as work and heat increase incessantly in time during nonequilibrium processes near steady states. In the long-time limit, the average values of work and heat become asymptotically equivalent to each other, since they only differ by a finite energy change in average. However, the fluctuation theorem (FT) for the heat is found not to hold with the equilibrium initial ensemble, while the FT for the work holds. This reveals an intriguing effect of everlasting initial memory stored in rare events. We revisit the problem of a Brownian particle in a harmonic potential dragged with a constant velocity, which is in contact with a thermal reservoir. The heat and work fluctuations are investigated with initial Boltzmann ensembles at temperatures generally different from the reservoir temperature. We find that, in the infinite-time limit, the FT for the work is fully recovered for arbitrary initial temperatures, while the heat fluctuations significantly deviate from the FT characteristics except for the infinite initial-temperature limit (a uniform initial ensemble). Furthermore, we succeed in calculating finite-time corrections to the heat and work distributions analytically, using the modified saddle point integral method recently developed by us. Interestingly, we find noncommutativity between the infinite-time limit and the infinite-initial-temperature limit for the probability distribution function (PDF) of the heat.

  17. Redox conversions of methemoglobin during redox cycling of quinones and aromatic nitrocompounds.

    Science.gov (United States)

    Cénas, N; Ollinger, K

    1994-11-15

    The study focused on the effects on various redox states of hemoglobin during NADPH: cytochrome P-450 reductase-catalyzed redox cycling of quinones and nitrocompounds. The following reactions involving quinone/semiquinone and methemoglobin/oxyhemoglobin redox couples were observed: (i) the direct oxidation of oxyhemoglobin by quinones, (ii) the reduction of methemoglobin by quinones, (ii) the reduction of methemoglobin during redox cycling of quinones and nitrocompounds was partially inhibited by superoxide dismutase, and (iii) the reoxidation of oxyhemoglobin by hydrogen peroxide, formed during redox cycling was accompanied by the formation of choleglobin. Hydrogen peroxide was produced during redox cycling, and upon depletion of hydrogen peroxide by catalase, the reduction of methemoglobin significantly prevailed over oxidation of oxyhemoglobin. Furthermore, the reduction of ferrylhemoglobin to oxyhemoglobin during redox cycling was about twice as slow as the reduction of methemoglobin. For a series of compounds possessing a single-electron reduction potential (E1(7)) between 0.01 and -0.355 V, the rate constants for methemoglobin reduction by their corresponding radicals was estimated to range from 4.1 x 10(5) to 7.6 x 10(7) M-1 S-1. Radicals of the nitrocompounds were approximately 10 times less reactive as compared to quinones possessing similar E1(7) values.

  18. Measurement of magnetic fluctuation induced energy transport

    International Nuclear Information System (INIS)

    Fiksel, G.; Prager, S.C.; Shen, W.; Stoneking, M.

    1993-11-01

    The local electron energy flux produced by magnetic fluctuations has been measured directly in the MST reversed field pinch (over the radial range r/a > 0.75). The flux, produced by electrons traveling parallel to a fluctuating magnetic field, is obtained from correlation between the fluctuations in the parallel heat flux and the radial magnetic field. The fluctuation induced flux is large (100 kW/cm 2 ) in the ''core'' (r/a 2 ) in the edge

  19. Reverse Engineering Applied to Red Human Hair Pheomelanin Reveals Redox-Buffering as a Pro-Oxidant Mechanism.

    Science.gov (United States)

    Kim, Eunkyoung; Panzella, Lucia; Micillo, Raffaella; Bentley, William E; Napolitano, Alessandra; Payne, Gregory F

    2015-12-16

    Pheomelanin has been implicated in the increased susceptibility to UV-induced melanoma for people with light skin and red hair. Recent studies identified a UV-independent pathway to melanoma carcinogenesis and implicated pheomelanin's pro-oxidant properties that act through the generation of reactive oxygen species and/or the depletion of cellular antioxidants. Here, we applied an electrochemically-based reverse engineering methodology to compare the redox properties of human hair pheomelanin with model synthetic pigments and natural eumelanin. This methodology exposes the insoluble melanin samples to complex potential (voltage) inputs and measures output response characteristics to assess redox activities. The results demonstrate that both eumelanin and pheomelanin are redox-active, they can rapidly (sec-min) and repeatedly redox-cycle between oxidized and reduced states, and pheomelanin possesses a more oxidative redox potential. This study suggests that pheomelanin's redox-based pro-oxidant activity may contribute to sustaining a chronic oxidative stress condition through a redox-buffering mechanism.

  20. Quantum inflaton, primordial perturbations, and CMB fluctuations

    International Nuclear Information System (INIS)

    Cao, F.J.; Vega, H.J. de; Sanchez, N.G.

    2004-01-01

    We compute the primordial scalar, vector and tensor metric perturbations arising from quantum field inflation. Quantum field inflation takes into account the nonperturbative quantum dynamics of the inflaton consistently coupled to the dynamics of the (classical) cosmological metric. For chaotic inflation, the quantum treatment avoids the unnatural requirements of an initial state with all the energy in the zero mode. For new inflation it allows a consistent treatment of the explosive particle production due to spinodal instabilities. Quantum field inflation (under conditions that are the quantum analog of slow-roll) leads, upon evolution, to the formation of a condensate starting a regime of effective classical inflation. We compute the primordial perturbations taking the dominant quantum effects into account. The results for the scalar, vector and tensor primordial perturbations are expressed in terms of the classical inflation results. For a N-component field in a O(N) symmetric model, adiabatic fluctuations dominate while isocurvature or entropy fluctuations are negligible. The results agree with the current Wilkinson Microwave Anisotropy Probe observations and predict corrections to the power spectrum in classical inflation. Such corrections are estimated to be of the order of (m 2 /NH 2 ), where m is the inflaton mass and H the Hubble constant at the moment of horizon crossing. An upper estimate turns to be about 4% for the cosmologically relevant scales. This quantum field treatment of inflation provides the foundations to the classical inflation and permits to compute quantum corrections to it

  1. Effects of temporal fluctuations on mixing

    Science.gov (United States)

    Pool, Maria; Dentz, Marco; Post, Vincent E. A.; Simmons, Craig T.

    2016-04-01

    Mixing and dispersion in coastal aquifers are strongly influenced by periodic temporal flow fluctuations on multiple time-scales ranging from days (tides), seasons (pumping and recharge) to glacial cycles (regression and transgressions). Transient forcing effects lead to a complex space- ant time-dependent flow response which induces enhanced spreading and mixing of a dissolved substance. We study effective mixing and solute transport in temporally fluctuating one-dimensional flow for a stable stratification of two fluids of different density. We derive explicit expressions for the concentration distribution and variance to identify the controls and obtain realistic predictions of the coupling between mixing and oscillatory transient flow. We find that the magnitude of transient-driven mixing is mainly controlled by the hydraulic diffusivity, the period and the initial interface location. We also find a spatial dependence of the effective dispersion coefficient which at long times causes the concentration profile to become asymmetric. Sand column experiments under well-controlled laboratory conditions are presented to validate the theoretical effective model defined. The proposed formulation is found to provide very good predictions and correctly reproduces the experimental mixing dynamics.

  2. Fluctuating selection on basal metabolic rate.

    Science.gov (United States)

    Nilsson, Johan F; Nilsson, Jan-Åke

    2016-02-01

    BMR (Basal metabolic rate) is an important trait in animal life history as it represents a significant part of animal energy budgets. BMR has also been shown to be positively related to sustainable work rate and maximal thermoregulatory capacity. To this date, most of the studies have focused on the causes of interspecific and intraspecific variation in BMR, and fairly little is known about the fitness consequences of different metabolic strategies. In this study, we show that winter BMR affects local survival in a population of wild blue tits (Cyanistes caeruleus), but that the selection direction differs between years. We argue that this fluctuating selection is probably a consequence of varying winter climate with a positive relation between survival and BMR during cold and harsh conditions, but a negative relation during mild winters. This fluctuating selection can not only explain the pronounced variation in BMR in wild populations, but will also give us new insights into how energy turnover rates can shape the life-history strategies of animals. Furthermore, the study shows that the process of global warming may cause directional selection for a general reduction in BMR, affecting the general life-history strategy on the population level.

  3. Electrostatic fluctuation in Low-β plasmas

    International Nuclear Information System (INIS)

    Krane, B

    1997-11-01

    The thesis gives an overview, which by no means is complete, to two dimensional plasma flows. The plasma is taken to be strongly magnetized such that magnetic fields generated by internal currents are small compared to the background magnetic field. This requires that the magnetic pressure exerted by the ambient magnetic field is large compared to the pressure due to thermal fluctuations, i.e. low-β plasma. The author also assume low frequency electrostatic fluctuations with ω c i where ω c i is the ion gyro frequency. A brief introduction to nonlinear phenomena in two dimensional plasma flows has been presented. Particular attention was given to simple models describing flute and drift modes. Although the derivations of the model equations are based on different assumptions regarding the plasma conditions, the resulting equations exhibit similar behavior in some respects. For instance, both the simple guiding center model and the Hasegawa-Mima model have stable dipolar structures. The inverse cascade was also found in both models. However, it is evident that there are significant differences, first of all the Hasegawa-Mima model assumes a background density gradient which makes it an inhomogeneous model. Secondly, in this model the electrons respond instantaneously to variations in the ion density by moving along the magnetic field, thereby introducing Debye shielding

  4. Wall pressure fluctuations in rod bundles

    International Nuclear Information System (INIS)

    Moeller, S.V.

    1990-01-01

    Microphones and hot wires were applied for the measurement of wall pressure fluctuations and velocity fluctuations in rod bundles with several aspect ratios. By means of auto and cross spectral density functions their interdependence was investigated. Results show that the pressure fluctuations in rod bundles are mainly associated with the phenomenon of quasi-periodic flow pulsations between subchannels. (author)

  5. Growth and decay of large fluctuations far from equilibrium

    Indian Academy of Sciences (India)

    Administrator

    tems which admit of stable dynamical attractors in absence of noise. An interesting 'detailed balance' like condition in the steady state which is manifested in the time reversal symmetry between growth and decay of fluctuation far from equilibrium, similar to what is observed in thermally equilibrated systems, is demonstrated ...

  6. Rewiring hydrogenase-dependent redox circuits in cyanobacteria

    Science.gov (United States)

    Ducat, Daniel C.; Sachdeva, Gairik; Silver, Pamela A.

    2011-01-01

    Hydrogenases catalyze the reversible reaction 2H+ + 2e-↔H2 with an equilibrium constant that is dependent on the reducing potential of electrons carried by their redox partner. To examine the possibility of increasing the photobiological production of hydrogen within cyanobacterial cultures, we expressed the [FeFe] hydrogenase, HydA, from Clostridium acetobutylicum in the non-nitrogen-fixing cyanobacterium Synechococcus elongatus sp. 7942. We demonstrate that the heterologously expressed hydrogenase is functional in vitro and in vivo, and that the in vivo hydrogenase activity is connected to the light-dependent reactions of the electron transport chain. Under anoxic conditions, HydA activity is capable of supporting light-dependent hydrogen evolution at a rate > 500-fold greater than that supported by the endogenous [NiFe] hydrogenase. Furthermore, HydA can support limited growth solely using H2 and light as the source of reducing equivalents under conditions where Photosystem II is inactivated. Finally, we demonstrate that the addition of exogenous ferredoxins can modulate redox flux in the hydrogenase-expressing strain, allowing for greater hydrogen yields and for dark fermentation of internal energy stores into hydrogen gas. PMID:21368150

  7. Synthesis of redox polymer nanobeads and nanocomposites for glucose biosensors.

    Science.gov (United States)

    Wang, Jen-Yuan; Chen, Lin-Chi; Ho, Kuo-Chuan

    2013-08-28

    Redox polymer nanobeads of branched polyethylenimine binding with ferrocene (BPEI-Fc) were synthesized using a simple chemical process. The functionality and morphology of the redox polymer nanobeads were investigated by Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). This hydrophilic redox nanomaterial could be mixed with glucose oxidase (GOx) for drop-coating on a screen-printed carbon electrode (SPCE) for glucose sensing application. Electrochemical properties of the BPEI-Fc/GOx/SPCE prepared under different conditions were studied by cyclic voltammetry (CV). On the basis of these CV results, the synthetic condition of the BPEI-Fc/GOx/SPCE could be optimized. By incorporating conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), the performance of a redox polymer nanobead–based enzyme electrode could be further improved. The influence of PEDOT:PSS on the nanocomposite enzyme electrode was discussed from the aspects of the apparent electron diffusion coefficient (D(app)) and the charge transfer resistance (R(ct)). The glucose-sensing sensitivity of the BPEI-Fc/PEDOT:PSS/GOx/SPCE is calculated to be 66 μA mM(–1) cm(–2), which is 2.5 times higher than that without PEDOT:PSS. The apparent Michaelis constant (K(M)(app)) of the BPEI-Fc/PEDOT:PSS/GOx/SPCE estimated by the Lineweaver–Burk plot is 2.4 mM, which is much lower than that of BPEI-Fc/GOx/SPCE (11.2 mM). This implies that the BPEI-Fc/PEDOT:PSS/GOx/SPCE can catalytically oxidize glucose in a more efficient way. The interference test was carried out by injection of glucose and three common interferences: ascorbic acid (AA), dopamine (DA), and uric acid (UA) at physiological levels. The interferences of DA (4.2%) and AA (7.8%) are acceptable and the current response to UA (1.6%) is negligible, compared to the current response to glucose.

  8. On the importance of hydroquinone/p-quinone redox system in the photoyellowing of mechanical pulps

    Science.gov (United States)

    Umesh P. Agarwal

    1999-01-01

    In the area of photoyellowing of mechanical pulps, recently obtained experimental evidence has shown that hydroquinone/p-quinone redox couple is present in lignin-rich mechanical pulps. It was also noted that compared to a control pulp the concentration of p-quinones was significantly higher in a photoyellowed pulp. Under ambient conditions, upon exposure to light, the...

  9. Effects of iron concentration and redox states on failure of boron-free ...

    Indian Academy of Sciences (India)

    Effects of iron concentration and redox states on failure of boron-free E-glass fibres under applied stress in different conditions ... Materials, Donghua University, Shanghai 201620, China; Material Research Center, Missouri University of Science and Technology, Rolla 65409, USA; Fiber Glass Science and Technology, ...

  10. In Situ Redox Manipulation Field Injection Test Report - Hanford 100-H Area

    Energy Technology Data Exchange (ETDEWEB)

    Fruchter, J.S.; Amonette, J.E.; Cole, C.R. [and others

    1996-11-01

    This report presents results of an In Situ Redox Manipulation (ISRM) Field Injection Withdrawal Test performed at the 100-H Area of the US. Department of Energy`s (DOE`s) Hanford Site in Washington State in Fiscal Year 1996 by researchers at Pacific Northwest National Laboratory (PNNL). The test is part of the overall ISRM project, the purpose of which is to determine the potential for remediating contaminated groundwater with a technology based on in situ manipulation of subsurface reduction-oxidation (redox) conditions. The ISRM technology would be used to treat subsurface contaminants in groundwater zones at DOE sites.

  11. In Situ Redox Manipulation Field Injection Test Report - Hanford 100-H Area

    International Nuclear Information System (INIS)

    Fruchter, J.S.; Amonette, J.E.; Cole, C.R.

    1996-11-01

    This report presents results of an In Situ Redox Manipulation (ISRM) Field Injection Withdrawal Test performed at the 100-H Area of the US. Department of Energy's (DOE's) Hanford Site in Washington State in Fiscal Year 1996 by researchers at Pacific Northwest National Laboratory (PNNL). The test is part of the overall ISRM project, the purpose of which is to determine the potential for remediating contaminated groundwater with a technology based on in situ manipulation of subsurface reduction-oxidation (redox) conditions. The ISRM technology would be used to treat subsurface contaminants in groundwater zones at DOE sites

  12. TEMPOL increases NAD+ and improves redox imbalance in obese mice

    OpenAIRE

    Yamato, Mayumi; Kawano, Kimika; Yamanaka, Yuki; Saiga, Misako; Yamada, Ken-ichi

    2016-01-01

    Continuous energy conversion is controlled by reduction–oxidation (redox) processes. NAD+ and NADH represent an important redox couple in energy metabolism. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) is a redox-cycling nitroxide that promotes the scavenging of several reactive oxygen species (ROS) and is reduced to hydroxylamine by NADH. TEMPOL is also involved in NAD+ production in the ascorbic acid–glutathione redox cycle. We utilized the chemical properties of TEMPOL to invest...

  13. Study of thermal - hydraulic sensors signal fluctuations in PWR

    International Nuclear Information System (INIS)

    Hennion, F.

    1987-10-01

    This thesis deals with signal fluctuations of thermal-hydraulic sensors in the main coolant primary of a pressurized water reactor. The aim of this work is to give a first response about the potentiality of use of these noise signals for the functionning monitoring. Two aspects have been studied: - the modelisation of temperature fluctuations of core thermocouples, by a Monte-Carlo method, gives the main characteristics of these signals and their domain of application. - the determination of eigenfrequency in the primary by an acoustic representation could permit the monitoring of local and global thermo-hydraulic conditions [fr

  14. MICROSCALE METABOLIC, REDOX AND ABIOTIC REACTIONS IN HANFORD 300 AREA SUBSURFACE SEDIMENTS

    Energy Technology Data Exchange (ETDEWEB)

    Beyenal, Haluk [WSU; McLEan, Jeff [JCVI; Majors, Paul [PNNL; Fredrickson, Jim [PNNL

    2013-11-14

    The Hanford 300 Area is a unique site due to periodic hydrologic influence of river water resulting in changes in groundwater elevation and flow direction. This area is also highly subject to uranium remobilization, the source of which is currently believed to be the region at the base of the vadose zone that is subject to period saturation due to the changes in the water levels in the Columbia River. We found that microbial processes and redox and abiotic reactions which operate at the microscale were critical to understanding factors controlling the macroscopic fate and transport of contaminants in the subsurface. The combined laboratory and field research showed how microscale conditions control uranium mobility and how biotic, abiotic and redox reactions relate to each other. Our findings extended the current knowledge to examine U(VI) reduction and immobilization using natural 300 Area communities as well as selected model organisms on redox-sensitive and redox-insensitive minerals. Using innovative techniques developed specifically to probe biogeochemical processes at the microscale, our research expanded our current understanding of the roles played by mineral surfaces, bacterial competition, and local biotic, abiotic and redox reaction rates on the reduction and immobilization of uranium.

  15. Intracellular Redox Compartmentation and ROS-Related Communication in Regulation and Signaling.

    Science.gov (United States)

    Noctor, Graham; Foyer, Christine H

    2016-07-01

    Recent years have witnessed enormous progress in understanding redox signaling related to reactive oxygen species (ROS) in plants. The consensus view is that such signaling is intrinsic to many developmental processes and responses to the environment. ROS-related redox signaling is tightly wedded to compartmentation. Because membranes function as barriers, highly redox-active powerhouses such as chloroplasts, peroxisomes, and mitochondria may elicit specific signaling responses. However, transporter functions allow membranes also to act as bridges between compartments, and so regulated capacity to transmit redox changes across membranes influences the outcome of triggers produced at different locations. As well as ROS and other oxidizing species, antioxidants are key players that determine the extent of ROS accumulation at different sites and that may themselves act as signal transmitters. Like ROS, antioxidants can be transported across membranes. In addition, the intracellular distribution of antioxidative enzymes may be modulated to regulate or facilitate redox signaling appropriate to the conditions. Finally, there is substantial plasticity in organellar shape, with extensions such as stromules, peroxules, and matrixules playing potentially crucial roles in organelle-organelle communication. We provide an overview of the advances in subcellular compartmentation, identifying the gaps in our knowledge and discussing future developments in the area. © 2016 American Society of Plant Biologists. All Rights Reserved.

  16. Protein Redox Modification as a Cellular Defense Mechanism against Tissue Ischemic Injury

    Directory of Open Access Journals (Sweden)

    Liang-Jun Yan

    2014-01-01

    Full Text Available Protein oxidative or redox modifications induced by reactive oxygen species (ROS or reactive nitrogen species (RNS not only can impair protein function, but also can regulate and expand protein function under a variety of stressful conditions. Protein oxidative modifications can generally be classified into two categories: irreversible oxidation and reversible oxidation. While irreversible oxidation usually leads to protein aggregation and degradation, reversible oxidation that usually occurs on protein cysteine residues can often serve as an “on and off” switch that regulates protein function and redox signaling pathways upon stress challenges. In the context of ischemic tolerance, including preconditioning and postconditioning, increasing evidence has indicated that reversible cysteine redox modifications such as S-sulfonation, S-nitrosylation, S-glutathionylation, and disulfide bond formation can serve as a cellular defense mechanism against tissue ischemic injury. In this review, I highlight evidence of cysteine redox modifications as protective measures in ischemic injury, demonstrating that protein redox modifications can serve as a therapeutic target for attenuating tissue ischemic injury. Prospectively, more oxidatively modified proteins will need to be identified that can play protective roles in tissue ischemic injury, in particular, when the oxidative modifications of such identified proteins can be enhanced by pharmacological agents or drugs that are available or to be developed.

  17. Redox characteristics of the eukaryotic cytosol

    DEFF Research Database (Denmark)

    López-Mirabal, H Reynaldo; Winther, Jakob R

    2007-01-01

    organism, Saccharomyces cerevisiae, where the combination of genetic and biochemical approaches has brought us furthest in understanding the mechanisms underlying cellular redox regulation. It has been shown in yeast that, in addition to the enzyme glutathione reductase, other mechanisms may exist...

  18. Investigating improvements on redox flow batteries

    CSIR Research Space (South Africa)

    Swartbooi, AM

    2006-09-01

    Full Text Available storage devices coupled to most of their applications. Lead-acid batteries have long been used as the most economical option to store electricity in many small scale applications, but lately more interest have been shown in redox flow batteries. The low...

  19. Methods for using redox liposome biosensors

    Science.gov (United States)

    Cheng, Quan; Stevens, Raymond C.

    2002-01-01

    The present invention provides methods and compositions for detecting the presence of biologically-important analytes by using redox liposome biosensors. In particular, the present invention provides liposome/sol-gel electrodes suitable for the detection of a wide variety of organic molecules, including but not limited to bacterial toxins.

  20. Redox regulation in cancer stem cells

    Science.gov (United States)

    Reactive oxygen species (ROS) and ROS-dependent (redox regulation) signaling pathways and transcriptional activities are thought to be critical in stem cell self-renewal and differentiation during growth and organogenesis. Aberrant ROS burst and dysregulation of those ROS-dependent cellular processe...

  1. Redox Control of Skeletal Muscle Regeneration.

    Science.gov (United States)

    Le Moal, Emmeran; Pialoux, Vincent; Juban, Gaëtan; Groussard, Carole; Zouhal, Hassane; Chazaud, Bénédicte; Mounier, Rémi

    2017-08-10

    Skeletal muscle shows high plasticity in response to external demand. Moreover, adult skeletal muscle is capable of complete regeneration after injury, due to the properties of muscle stem cells (MuSCs), the satellite cells, which follow a tightly regulated myogenic program to generate both new myofibers and new MuSCs for further needs. Although reactive oxygen species (ROS) and reactive nitrogen species (RNS) have long been associated with skeletal muscle physiology, their implication in the cell and molecular processes at work during muscle regeneration is more recent. This review focuses on redox regulation during skeletal muscle regeneration. An overview of the basics of ROS/RNS and antioxidant chemistry and biology occurring in skeletal muscle is first provided. Then, the comprehensive knowledge on redox regulation of MuSCs and their surrounding cell partners (macrophages, endothelial cells) during skeletal muscle regeneration is presented in normal muscle and in specific physiological (exercise-induced muscle damage, aging) and pathological (muscular dystrophies) contexts. Recent advances in the comprehension of these processes has led to the development of therapeutic assays using antioxidant supplementation, which result in inconsistent efficiency, underlying the need for new tools that are aimed at precisely deciphering and targeting ROS networks. This review should provide an overall insight of the redox regulation of skeletal muscle regeneration while highlighting the limits of the use of nonspecific antioxidants to improve muscle function. Antioxid. Redox Signal. 27, 276-310.

  2. Redox flow cell energy storage systems

    Science.gov (United States)

    Thaller, L. H.

    1979-01-01

    NASA-Redox systems are electrochemical storage devices that use two fully soluble Redox couples, anode and cathode fluids, as active electrode materials separated by a highly selective ion exchange membrane. The reactants are contained in large storage tanks and pumped through a stack of Redox flow cells where the electrochemical reactions (reduction and oxidation) take place at porous carbon felt electrodes. A string or stack of these power producing cells is connected in series in a bipolar manner. Redox energy storage systems promise to be inexpensive and possess many features that provide for flexible design, long life, high reliability and minimal operation and maintenance costs. These features include independent sizing of power and storage capacity requirements and inclusion within the cell stack of a cell that monitors the state of charge of the system as a whole, and a rebalance cell which permits continuous correction to be made for minor side reactions that would tend to result in the anode fluid and cathode fluids becoming electrochemically out of balance. These system features are described and discussed.

  3. Solvent fluctuations at hydrophobic, hydrophilic, and electrochemical interfaces

    Science.gov (United States)

    Willard, Adam Phillip

    nanoscale hydrophobic particles in water. We show how solvent degrees of freedom are involved in the process. By using tools of transition path sampling, we elucidate the reaction coordinates describing the assembly. In accord with earlier expectations, we find that fluctuations of the liquid-vapor-like interface surrounding the solutes are significant, in this case leading to the formation of a vapor tunnel between the two solute particles. This tunnel accelerates assembly. Finally, the results of molecular dynamics simulations of the properties of water in an aqueous ionic solution close to an interface with a model metallic electrode are described. In the simulations the electrode behaves as an ideally polarizable hydrophilic metal, supporting image-charge interactions with charged species, and it is maintained at a constant electrical potential with respect to the solution so that the model is a textbook representation of an electrochemical interface through which no current is passing. We show how water is strongly attracted to and ordered at the electrode surface. This ordering is different to the structure that might be imagined from continuum models of electrode interfaces. Further, this ordering significantly affects the probability of ions reaching the surface. We describe the concomitant motion and configurations of the water and ions as functions of the electrode potential, and we analyze the length scales over which ionic atmospheres fluctuate. The statistics of these fluctuations depend upon surface structure and ionic strength. The fluctuations are large---sufficiently so that the mean ionic atmosphere is a poor descriptor of the aqueous environment near a metal surface. The importance of this finding for a description of electrochemical reactions is examined by calculating, directly from the simulation, Marcus free-energy profiles for transfer of charge between the electrode and a redox species in the solution and comparing the results with the predictions

  4. Bimetallic redox synergy in oxidative palladium catalysis.

    Science.gov (United States)

    Powers, David C; Ritter, Tobias

    2012-06-19

    Polynuclear transition metal complexes, which are embedded in the active sites of many metalloenzymes, are responsible for effecting a diverse array of oxidation reactions in nature. The range of chemical transformations remains unparalleled in the laboratory. With few noteworthy exceptions, chemists have primarily focused on mononuclear transition metal complexes in developing homogeneous catalysis. Our group is interested in the development of carbon-heteroatom bond-forming reactions, with a particular focus on identifying reactions that can be applied to the synthesis of complex molecules. In this context, we have hypothesized that bimetallic redox chemistry, in which two metals participate synergistically, may lower the activation barriers to redox transformations relevant to catalysis. In this Account, we discuss redox chemistry of binuclear Pd complexes and examine the role of binuclear intermediates in Pd-catalyzed oxidation reactions. Stoichiometric organometallic studies of the oxidation of binuclear Pd(II) complexes to binuclear Pd(III) complexes and subsequent C-X reductive elimination from the resulting binuclear Pd(III) complexes have confirmed the viability of C-X bond-forming reactions mediated by binuclear Pd(III) complexes. Metal-metal bond formation, which proceeds concurrently with oxidation of binuclear Pd(II) complexes, can lower the activation barrier for oxidation. We also discuss experimental and theoretical work that suggests that C-X reductive elimination is also facilitated by redox cooperation of both metals during reductive elimination. The effect of ligand modification on the structure and reactivity of binuclear Pd(III) complexes will be presented in light of the impact that ligand structure can exert on the structure and reactivity of binuclear Pd(III) complexes. Historically, oxidation reactions similar to those discussed here have been proposed to proceed via mononuclear Pd(IV) intermediates, and the hypothesis of mononuclear Pd

  5. Critical swelling of fluctuating capsules

    Science.gov (United States)

    Diamant, Haim; Haleva, Emir

    2009-03-01

    In many natural transport processes the solute molecules to be transported are encapsulated in semipermeable, flexible membrane vesicles of micron size. We study the swelling of such fluctuating capsules, as the number of encapsulated particles is increased, or the concentration of the outer solution is decreased. The approach to the maximum volume-to-area ratio and the associated buildup of membrane tension involve a continuous phase transition and follow universal scaling laws. The criticality and its features are model-independent, arising solely from the interplay between volume and surface degrees of freedom.ootnotetextE. Haleva and H. Diamant, Phys. Rev. Lett. 101, 078104 (2008).

  6. Noise and fluctuations an introduction

    CERN Document Server

    MacDonald, D K C

    2006-01-01

    An understanding of fluctuations and their role is both useful and fundamental to the study of physics. This concise study of random processes offers graduate students and research physicists a survey that encompasses both the relationship of Brownian Movement with statistical mechanics and the problem of irreversible processes. It outlines the basics of the physics involved, without the strictures of mathematical rigor.The three-part treatment starts with a general survey of Brownian Movement, including electrical Brownian Movement and ""shot-noise,"" Part two explores correlation, frequency

  7. Fluctuations in Overlapping Generations Economies

    DEFF Research Database (Denmark)

    Tvede, Mich

    In the present paper stationary pure-exchange overlapping generations economies with l  goods per date and m consumers per generation are considered. It is shown that for an open and dense set of utility functions there exist endowment vectors such that n-cycles exist for n = l +1 and l  = m....... The approach to existence of endogenous fluctuations is basic in the sense that the prime ingredients are the implicit function theorem and linear algebra. Moreover the approach is applied to show that for an open and dense set of utility functions there exist endowment vectors such that sunspot equilibria...

  8. Using a redox-sensitive phosphorescent probe for optical evaluation of an intracellular redox environment.

    Science.gov (United States)

    Liu, Shujuan; Zhou, Na; Chen, Zejing; Wei, Huanjie; Zhu, Yana; Guo, Song; Zhao, Qiang

    2017-01-01

    A reducing intracellular environment is necessary for living cells. Here a redox-sensitive phosphorescent probe Ir-NO has been developed for evaluating the redox environment in living cells. Upon addition of reducing molecules, such as glutathione and ascorbic acid, the phosphorescent intensity of the probe is turned on, and the emission lifetime is elongated evidently. Furthermore, this probe has been used for optical imaging of the intracellular reducing environment by utilizing confocal laser scanning microscopy and phosphorescence lifetime imaging microscopy.

  9. An Objective Fluctuation Score for Parkinson's Disease

    Science.gov (United States)

    Horne, Malcolm K.; McGregor, Sarah; Bergquist, Filip

    2015-01-01

    Introduction Establishing the presence and severity of fluctuations is important in managing Parkinson’s Disease yet there is no reliable, objective means of doing this. In this study we have evaluated a Fluctuation Score derived from variations in dyskinesia and bradykinesia scores produced by an accelerometry based system. Methods The Fluctuation Score was produced by summing the interquartile range of bradykinesia scores and dyskinesia scores produced every 2 minutes between 0900-1800 for at least 6 days by the accelerometry based system and expressing it as an algorithm. Results This Score could distinguish between fluctuating and non-fluctuating patients with high sensitivity and selectivity and was significant lower following activation of deep brain stimulators. The scores following deep brain stimulation lay in a band just above the score separating fluctuators from non-fluctuators, suggesting a range representing adequate motor control. When compared with control subjects the score of newly diagnosed patients show a loss of fluctuation with onset of PD. The score was calculated in subjects whose duration of disease was known and this showed that newly diagnosed patients soon develop higher scores which either fall under or within the range representing adequate motor control or instead go on to develop more severe fluctuations. Conclusion The Fluctuation Score described here promises to be a useful tool for identifying patients whose fluctuations are progressing and may require therapeutic changes. It also shows promise as a useful research tool. Further studies are required to more accurately identify therapeutic targets and ranges. PMID:25928634

  10. Conserved number fluctuations in a hadron resonance gas model

    International Nuclear Information System (INIS)

    Garg, P.; Mishra, D.K.; Netrakanti, P.K.; Mohanty, B.; Mohanty, A.K.; Singh, B.K.; Xu, N.

    2013-01-01

    Net-baryon, net-charge and net-strangeness number fluctuations in high energy heavy-ion collisions are discussed within the framework of a hadron resonance gas (HRG) model. Ratios of the conserved number susceptibilities calculated in HRG are being compared to the corresponding experimental measurements to extract information about the freeze-out condition and the phase structure of systems with strong interactions. We emphasize the importance of considering the actual experimental acceptances in terms of kinematics (pseudorapidity (η) and transverse momentum (p T )), the detected charge state, effect of collective motion of particles in the system and the resonance decay contributions before comparisons are made to the theoretical calculations. In this work, based on HRG model, we report that the net-baryon number fluctuations are least affected by experimental acceptances compared to the net-charge and net-strangeness number fluctuations

  11. Redox models in chemistry :  A depiction of the conceptions held by secondary school students of redox reactions

    OpenAIRE

    Österlund, Lise-Lotte

    2010-01-01

    According to previous research, students show difficulties in learning redox reactions. By the historical development different redox models exist to explain redox reactions, the oxygen model, the hydrogen model, the electron model and the oxidation number model. This thesis reports about three studies concerning conceptions held by secondary school students of redox reactions. A textbook analysis is also included in the thesis. The first study was an investigation of the students’ use of red...

  12. Selective dendritic susceptibility to bioenergetic, excitotoxic and redox perturbations in cortical neurons.

    Science.gov (United States)

    Hasel, Philip; Mckay, Sean; Qiu, Jing; Hardingham, Giles E

    2015-09-01

    Neurodegenerative and neurological disorders are often characterised by pathological changes to dendrites, in advance of neuronal death. Oxidative stress, energy deficits and excitotoxicity are implicated in many such disorders, suggesting a potential vulnerability of dendrites to these situations. Here we have studied dendritic vs. somatic responses of primary cortical neurons to these types of challenges in real-time. Using a genetically encoded indicator of intracellular redox potential (Grx1-roGFP2) we found that, compared to the soma, dendritic regions exhibited more dramatic fluctuations in redox potential in response to sub-lethal ROS exposure, and existed in a basally more oxidised state. We also studied the responses of dendritic and somatic regions to excitotoxic NMDA receptor activity. Both dendritic and somatic regions experienced similar increases in cytoplasmic Ca²⁺. Interestingly, while mitochondrial Ca²⁺ uptake and initial mitochondrial depolarisation were similar in both regions, secondary delayed mitochondrial depolarisation was far weaker in dendrites, potentially as a result of less NADH depletion. Despite this, ATP levels were found to fall faster in dendritic regions. Finally we studied the responses of dendritic and somatic regions to energetically demanding action potential burst activity. Burst activity triggered PDH dephosphorylation, increases in oxygen consumption and cellular NADH:NAD ratio. Compared to somatic regions, dendritic regions exhibited a smaller degree of mitochondrial Ca²⁺ uptake, lower fold-induction of NADH and larger reduction in ATP levels. Collectively, these data reveal that dendritic regions of primary neurons are vulnerable to greater energetic and redox fluctuations than the cell body, which may contribute to disease-associated dendritic damage. This article is part of a Special Issue entitled: 13th European Symposium on Calcium. Copyright © 2014. Published by Elsevier B.V.

  13. Root-Zone Redox Dynamics - In Search for the Cause of Damage to Treated-Wastewater Irrigated Orchards in Clay Soils

    Science.gov (United States)

    Yalin, David; Shenker, Moshe; Schwartz, Amnon; Assouline, Shmuel; Tarchitzky, Jorge

    2016-04-01

    Treated wastewater (TW) has become a common source of water for agriculture. However recent findings raise concern regarding its use: a marked decrease (up to 40%) in yield appeared in orchards irrigated with TW compared with fresh water (FW) irrigated orchards. These detrimental effects appeared predominantly in orchards cultivated in clay soils. The association of the damage with clay soils rather than sandy soils led us to hypothesize that the damage is linked to soil aeration problems. We suspected that in clay soils, high sodium adsorption ratio (SAR) and high levels of organic material, both typical of TW, may jointly lead to an extreme decrease in soil oxygen levels, so as to shift soil reduction-oxidation (redox) state down to levels that are known to damage plants. Two-year continuous measurement of redox potential, pH, water tension, and oxygen were conducted in the root-zone (20-35 cm depth) of avocado trees planted in clay soil and irrigated with either TW or FW. Soil solution composition was sampled periodically in-situ and mineral composition was sampled in tree leaves and woody organs biannually. In dry periods the pe+pH values indicated oxic conditions (pe+pH>14), and the fluctuations in redox values were small in both TW and FW plots. Decreases in soil water tension following irrigation or rain were followed by drops in soil oxygen and pe+pH values. TW irrigated plots had significantly lower minimum pe+pH values compared with FW-irrigated plots, the most significant differences occurred during the irrigation season rather than the rain season. A linear correlation appeared between irrigation volume and reduction severity in TW-irrigated plots, but not in the FW plots, indicating a direct link to the irrigation regime in TW-irrigated plots. The minimum pe+pH values measured in the TW plots are indicative of suboxic conditions (9water tension and oxygen concentration levels. The consequences of our findings to plant health will be discussed, and

  14. Redox regulation and pro-oxidant reactions in the physiology of circadian systems.

    Science.gov (United States)

    Méndez, Isabel; Vázquez-Martínez, Olivia; Hernández-Muñoz, Rolando; Valente-Godínez, Héctor; Díaz-Muñoz, Mauricio

    2016-05-01

    Rhythms of approximately 24 h are pervasive in most organisms and are known as circadian. There is a molecular circadian clock in each cell sustained by a feedback system of interconnected "clock" genes and transcription factors. In mammals, the timing system is formed by a central pacemaker, the suprachiasmatic nucleus, in coordination with a collection of peripheral oscillators. Recently, an extensive interconnection has been recognized between the molecular circadian clock and the set of biochemical pathways that underlie the bioenergetics of the cell. A principle regulator of metabolic networks is the flow of electrons between electron donors and acceptors. The concomitant reduction and oxidation (redox) reactions directly influence the balance between anabolic and catabolic processes. This review summarizes and discusses recent findings concerning the mutual and dynamic interactions between the molecular circadian clock, redox reactions, and redox signaling. The scope includes the regulatory role played by redox coenzymes (NAD(P)+/NAD(P)H, GSH/GSSG), reactive oxygen species (superoxide anion, hydrogen peroxide), antioxidants (melatonin), and physiological events that modulate the redox state (feeding condition, circadian rhythms) in determining the timing capacity of the molecular circadian clock. In addition, we discuss a purely metabolic circadian clock, which is based on the redox enzymes known as peroxiredoxins and is present in mammalian red blood cells and in other biological systems. Both the timing system and the metabolic network are key to a better understanding of widespread pathological conditions such as the metabolic syndrome, obesity, and diabetes. Copyright © 2015 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

  15. Thermal crackling: study of the mechanical effects of quick temperature fluctuations on metallic surfaces

    International Nuclear Information System (INIS)

    Pradel, P.

    1984-05-01

    After a brief overview of the thermohydraulical conditions of liquid sodium leading to important temperature fluctuations near the metallic surfaces, the author examines the transfer modes of these fluctuations in the structure thickness and the long term mechanical effects. Dimensioning models based on thermal and metallurgical properties are under study for structures subject to such sodium loads [fr

  16. Entropic fluctuations in DNA sequences

    Science.gov (United States)

    Thanos, Dimitrios; Li, Wentian; Provata, Astero

    2018-03-01

    The Local Shannon Entropy (LSE) in blocks is used as a complexity measure to study the information fluctuations along DNA sequences. The LSE of a DNA block maps the local base arrangement information to a single numerical value. It is shown that despite this reduction of information, LSE allows to extract meaningful information related to the detection of repetitive sequences in whole chromosomes and is useful in finding evolutionary differences between organisms. More specifically, large regions of tandem repeats, such as centromeres, can be detected based on their low LSE fluctuations along the chromosome. Furthermore, an empirical investigation of the appropriate block sizes is provided and the relationship of LSE properties with the structure of the underlying repetitive units is revealed by using both computational and mathematical methods. Sequence similarity between the genomic DNA of closely related species also leads to similar LSE values at the orthologous regions. As an application, the LSE covariance function is used to measure the evolutionary distance between several primate genomes.

  17. Study of droplet flow in a T-shape microchannel with bottom wall fluctuation

    Science.gov (United States)

    Pang, Yan; Wang, Xiang; Liu, Zhaomiao

    2018-03-01

    Droplet generation in a T-shape microchannel, with a main channel width of 50 μm , side channel width of 25 μm, and height of 50 μm, is simulated to study the effects of the forced fluctuation of the bottom wall. The periodic fluctuations of the bottom wall are applied on the near junction part of the main channel in the T-shape microchannel. Effects of bottom wall's shape, fluctuation periods, and amplitudes on the droplet generation are covered in the research of this protocol. In the simulation, the average size is affected a little by the fluctuations, but significantly by the fixed shape of the deformed bottom wall, while the droplet size range is expanded by the fluctuations under most of the conditions. Droplet sizes are distributed in a periodic pattern with small amplitude along the relative time when the fluctuation is forced on the bottom wall near the T-junction, while the droplet emerging frequency is not varied by the fluctuation. The droplet velocity is varied by the bottom wall motion, especially under the shorter period and the larger amplitude. When the fluctuation period is similar to the droplet emerging period, the droplet size is as stable as the non-fluctuation case after a development stage at the beginning of flow, while the droplet velocity is varied by the moving wall with the scope up to 80% of the average velocity under the conditions of this investigation.

  18. Application of genetically encoded redox biosensors to measure dynamic changes in the glutathione, bacillithiol and mycothiol redox potentials in pathogenic bacteria.

    Science.gov (United States)

    Tung, Quach Ngoc; Linzner, Nico; Loi, Vu Van; Antelmann, Haike

    2018-02-15

    Gram-negative bacteria utilize glutathione (GSH) as their major LMW thiol. However, most Gram-positive bacteria do not encode enzymes for GSH biosynthesis and produce instead alternative LMW thiols, such as bacillithiol (BSH) and mycothiol (MSH). BSH is utilized by Firmicutes and MSH is the major LMW thiol of Actinomycetes. LMW thiols are required to maintain the reduced state of the cytoplasm, but are also involved in virulence mechanisms in human pathogens, such as Staphylococcus aureus, Mycobacterium tuberculosis, Streptococcus pneumoniae, Salmonella enterica subsp. Typhimurium and Listeria monocytogenes. Infection conditions often cause perturbations of the intrabacterial redox balance in pathogens, which is further affected under antibiotics treatments. During the last years, novel glutaredoxin-fused roGFP2 biosensors have been engineered in many eukaryotic organisms, including parasites, yeast, plants and human cells for dynamic live-imaging of the GSH redox potential in different compartments. Likewise bacterial roGFP2-based biosensors are now available to measure the dynamic changes in the GSH, BSH and MSH redox potentials in model and pathogenic Gram-negative and Gram-positive bacteria. In this review, we present an overview of novel functions of the bacterial LMW thiols GSH, MSH and BSH in pathogenic bacteria in virulence regulation. Moreover, recent results about the application of genetically encoded redox biosensors are summarized to study the mechanisms of host-pathogen interactions, persistence and antibiotics resistance. In particularly, we highlight recent biosensor results on the redox changes in the intracellular food-borne pathogen Salmonella Typhimurium as well as in the Gram-positive pathogens S. aureus and M. tuberculosis during infection conditions and under antibiotics treatments. These studies established a link between ROS and antibiotics resistance with the intracellular LMW thiol-redox potential. Future applications should be directed

  19. Redox control of iron biomineralization in Magnetospirillum magneticum AMB-1

    Science.gov (United States)

    Jones, Stephanie Rhianon

    use in vitro binding and synthesis studies with putative magnetite-templating proteins, the Mms6 family proteins, to show that they are competent to bind and stabilize non-thermodynamically stable faces of magnetite. We also use in vitro iron mineralization to show that the Mms6 family proteins can work together with the redox protein MamP to produce mixed-valent iron oxides from soluble Fe(II) species and to control mineral structure. Further studies with Mms7ct indicate that it and other Mms6 family proteins may play a more significant role in controlling magnetite mineral structure than previously hypothesized. Beyond simple control of size and shape of magnetite, it may also template the crystal lattice of the mineral itself similar to what has been observed with calcium biomineralization, where unstable crystal forms and phases of the mineral are stabilized by interaction with peptides and other macromolecules. We next set up and begin testing systems to engineer magnetotactic bacteria for the production of new functional materials. We replace the metal-binding C-terminus of native Mms6 family proteins in AMB-1 with peptides known to precipitate metal oxides in vitro under mild conditions. Initial characterization of the behavior of these constructs in AMB-1 has been carried out, although additional experiments are required to test whether they can enable formation of new materials in vivo. We also discuss developing a cellular biosensor based on the formation of a magnetic material in response to an analyte.

  20. Redox effects on the microbial degradation of refractory organic matter in marine sediments

    Science.gov (United States)

    Reimers, Clare E.; Alleau, Yvan; Bauer, James E.; Delaney, Jennifer; Girguis, Peter R.; Schrader, Paul S.; Stecher, Hilmar A.

    2013-11-01

    Microbially mediated reduction-oxidation (redox) reactions are often invoked as being the mechanisms by which redox state influences the degradation of sedimentary organic matter (OM) in the marine environment. To evaluate the effects of elevated, oscillating and reduced redox potentials on the fate of primarily aged, mineral-adsorbed OM contained in continental shelf sediments, we used microbial fuel cells to control redox state within and around marine sediments, without amending the sediments with reducing or oxidizing substances. We subsequently followed electron fluxes in the redox elevated and redox oscillating treatments, and related sediment chemical, isotopic and bacterial community changes to redox conditions over a 748-day experimental period. The electron fluxes of the elevated and oscillating redox cells were consistent with models of organic carbon (OC) oxidation with time-dependent first-order rate constants declining from 0.023 to 0.005 y-1, in agreement with rate constants derived from typical OC profiles and down core ages of offshore sediments, or from sulfate reduction rate measurements in similar sediments. Moreover, although cumulative electron fluxes were higher in the continuously elevated redox treatment, incremental rates of electron harvesting in the two treatments converged over the 2 year experiment. These similar rates were reflected in chemical indicators of OM metabolism such as dissolved OC and ammonia, and particulate OC concentrations, which were not significantly different among all treatments and controls over the experimental time-scale. In contrast, products of carbonate and opal dissolution and metal mobilization showed greater enrichments in sediments with elevated and oscillating redox states. Microbial community composition in anode biofilms and surrounding sediments was assessed via high-throughput 16S rRNA gene sequencing, and these analyses revealed that the elevated and oscillatory redox treatments led to the

  1. Mesoporous tungsten oxynitride as electrocatalyst for promoting redox reactions of vanadium redox couple and performance of vanadium redox flow battery

    Science.gov (United States)

    Lee, Wonmi; Jo, Changshin; Youk, Sol; Shin, Hun Yong; Lee, Jinwoo; Chung, Yongjin; Kwon, Yongchai

    2018-01-01

    For enhancing the performance of vanadium redox flow battery (VRFB), a sluggish reaction rate issue of V2+/V3+ redox couple evaluated as the rate determining reaction should be addressed. For doing that, mesoporous tungsten oxide (m-WO3) and oxyniride (m-WON) structures are proposed as the novel catalysts, while m-WON is gained by NH3 heat treatment of m-WO3. Their specific surface area, crystal structure, surface morphology and component analysis are measured using BET, XRD, TEM and XPS, while their catalytic activity for V2+/V3+ redox reaction is electrochemically examined. As a result, the m-WON shows higher peak current, smaller peak potential difference, higher electron transfer rate constant and lower charge transfer resistance than other catalysts, like the m-WO3, WO3 nanoparticle and mesoporous carbon, proving that it is superior catalyst. Regarding the charge-discharge curve tests, the VRFB single cell employing the m-WON demonstrates high voltage and energy efficiencies, high specific capacity and low capacity loss rate. The excellent results of m-WON are due to the reasons like (i) reduced energy band gap, (ii) reaction familiar surface functional groups and (ii) greater electronegativity.

  2. Temperature- and pH-dependent effect of lactate on in vitro redox stability of red meat myoglobins.

    Science.gov (United States)

    Nair, M N; Suman, S P; Li, S; Ramanathan, R; Mancini, R A

    2014-01-01

    Our objective was to evaluate the influence of lactate on in vitro redox stability and thermostability of beef, horse, pork, and sheep myoglobins. Lactate (200 mM) had no effect (P>0.05) on redox stability at physiological (pH7.4, 37°C) and meat (pH 5.6, 4°C) conditions. However, lactate increased (Pmeat conditions was species-specific (Pmeat condition suggests that the color stability of lactate-enhanced fresh meat is not due to direct interactions between the ingredient and the heme protein. © 2013.

  3. Bet Hedging against Demographic Fluctuations

    Science.gov (United States)

    Xue, BingKan; Leibler, Stanislas

    2017-09-01

    Biological organisms have to cope with stochastic variations in both the external environment and the internal population dynamics. Theoretical studies and laboratory experiments suggest that population diversification could be an effective bet-hedging strategy for adaptation to varying environments. Here we show that bet hedging can also be effective against demographic fluctuations that pose a trade-off between growth and survival for populations even in a constant environment. A species can maximize its overall abundance in the long term by diversifying into coexisting subpopulations of both "fast-growing" and "better-surviving" individuals. Our model generalizes statistical physics models of birth-death processes to incorporate dispersal, during which new populations are founded, and can further incorporate variations of local environments. In this way, we unify different bet-hedging strategies against demographic and environmental variations as a general means of adaptation to both types of uncertainties in population growth.

  4. Characterizing flow fluctuations with moments

    Directory of Open Access Journals (Sweden)

    Rajeev S. Bhalerao

    2015-03-01

    Full Text Available We present a complete set of multiparticle correlation observables for ultrarelativistic heavy-ion collisions. These include moments of the distribution of the anisotropic flow in a single harmonic and also mixed moments, which contain the information on correlations between event planes of different harmonics. We explain how all these moments can be measured using just two symmetric subevents separated by a rapidity gap. This presents a multi-pronged probe of the physics of flow fluctuations. For instance, it allows to test the hypothesis that event-plane correlations are generated by non-linear hydrodynamic response. We illustrate the method with simulations of events in A MultiPhase Transport (AMPT model.

  5. Fluctuating nonlinear hydrodynamics of flocking

    Science.gov (United States)

    Yadav, Sunil Kumar; Das, Shankar P.

    2018-03-01

    Starting from a microscopic model, the continuum field theoretic description of the dynamics of a system of active ingredients or "particles" is presented. The equations of motion for the respective collective densities of mass and momentum follow exactly from that of a single element in the flock. The single-particle dynamics has noise and anomalous momentum dependence in its frictional terms. The equations for the collective densities are averaged over a local equilibrium distribution to obtain the corresponding coarse grained equations of fluctuating nonlinear hydrodynamics (FNH). The latter are the equations used frequently for describing active systems on the basis of intuitive arguments. The transport coefficients which appear in the macroscopic FNH equations are determined in terms of the parameters of the microscopic dynamics.

  6. Biosignature Preservation Vulnerability Associated with Stress Response Metabolic Redox Mode Switching in a Mars Analogue Coupled Microbial Mat Transiting Near-Space

    Science.gov (United States)

    Archer, R.; Ralat, A.

    2016-05-01

    Examination of a coupled microbial mat recovered from Death Valley failed to detect rosickyte, both before and after exposure to near-space conditions; associated redox proxies suggest diagenesis caused by rapid adaptive microbial stress response.

  7. Origin and nature of spontaneous shape fluctuations in "small" nanoparticles.

    Science.gov (United States)

    Yang, Ying; Zhang, Hao; Douglas, Jack F

    2014-07-22

    Normally chemically inert materials such as Au have been found to be catalytically active in the form of particles whose size is about 1 nm. Direct and indirect observations of various types of metal nanoparticles (NPs) in this size range, under catalytically relevant conditions for fuel-cell operation and catalysis, have indicated that such "small" particles can exhibit large spontaneous shape fluctuations and significant changes in shape and chemical activity in response to alterations in environmental conditions. NPs also normally exhibit facile coalescence when in proximity, impacting their stability and reactivity in applications. We perform molecular dynamics simulations on Ni nanoparticles, a commonly used NP in catalytic applications and carbon nanotube growth, in the ≈1 nm size regime where large-scale shape fluctuations have been observed experimentally. An analysis of the large-scale shape fluctuations observed in our simulations of these "small" NPs indicates that they are accompanied by collective motion of Ni atoms through the NP center, and we quantify these dynamic structures and their impact on NP shape. In contrast, stringlike collective atomic motion is confined to the NP interfacial region of NPs having a diameter greater than a few nanometers, and correspondingly, the overall NP shape remains roughly spherical, a case studied in our prior Ni NP simulations. Evidently, the large spontaneous NP shape fluctuations reflect a change in character of the collective atomic dynamics when the NPs become critically small in size.

  8. Interplanetary Alfvenic fluctuations: A stochastic model

    International Nuclear Information System (INIS)

    Barnes, A.

    1981-01-01

    The strong alignment of the average directions of minimum magnetic variance and mean magnetic field in interplanetary Alfvenic fluctuations is inconsistent with the usual wave-propagation models. We investigate the concept of minimum variance for nonplanar Alfvenic fluctuations in which the field direction varies stochastically. It is found that the tendency of the minimum variance and mean field directions to be aligned may be purely a consequence of the randomness of the field direction. In particular, a well-defined direction of minimum variance does not imply that the fluctuations are necessarily planar. The fluctuation power spectrum is a power law for frequencies much higher than the inverse of the correlation time. The probability distribution of directions a randomly fluctuating field of constant magnitude is calculated. A new approach for observational studies of interplanetary fluctuations is suggested

  9. Anion permselective membrane. [For redox fuel cells

    Energy Technology Data Exchange (ETDEWEB)

    Alexander, S.S.; Hodgdon, R.B.

    1978-01-01

    Experimental anion permeselective membranes were improved and characterized for use as separators in a chemical redox, power storage cell being developed at the NASA Lewis Research Center. The goal of minimal Fe/sup +3/ ion transfer was achieved for each candidate membrane system. Minimal membrane resistivity was demonstrated by reduction of film thickness using synthetic backing materials but usefulness of thin membranes was limited by the scarcity of compatible fabrics. The most durable and useful backing fabrics were modacrylics. One membrane, a copolymer of 4 vinylpyridine and vinyl benzylchloride was outstanding in overall electrochemical and physical properties. Long term (1000 hrs) membrane chemical and thermal durability in redox environment was shown by three candidate polymers and two membranes. The remainder had good durability at ambient temperature. Manufacturing capability was demonstrated for large scale production of membrane sheets 5.5 ft/sup 2/ in area for two candidate systems.

  10. Redox potential driven aeration during very-high-gravity ethanol fermentation by using flocculating yeast

    OpenAIRE

    Chen-Guang Liu; Xue-Mi Hao; Yen-Han Lin; Feng-Wu Bai

    2016-01-01

    Ethanol fermentation requires oxygen to maintain high biomass and cell viability, especially under very-high-gravity (VHG) condition. In this work, fermentation redox potential (ORP) was applied to drive the aeration process at low dissolved oxygen (DO) levels, which is infeasible to be regulated by a DO sensor. The performance and characteristics of flocculating yeast grown under 300 and 260?g glucose/L conditions were subjected to various aeration strategies including: no aeration; controll...

  11. Fluctuations in Supercooled Fluids and Ionic Solutions

    Science.gov (United States)

    Thorpe, Dayton Gray

    An overview of five studies is presented in two parts. The first part presents two studies of supercooled fluids. The second part presents three studies of water and aqueous solutions. Each study seeks a minimal model of a condensed matter system. In the first study, kinetically constrained models (KCM's) are compared to alternative theories of the glass transition in high dimensions. Dimensionality is used as a parameter to tune the connectivity of a lattice, where a higher dimensional model has more interactions between neighboring sites. This study finds that KCM's outperform alternative theories in high dimensions. The second study explores the possibility that bacteria have evolved to exploit the glass transition to enter a dormant state when environmental conditions are unfavorable. Although the available evidence shows that the bacterial cytoplasm does not meet the strict definition of a fragile glass former, much of its behavior is similar to and can be described using close analogies with the glass transition. In the second part, the third study describes the molecular mechanisms that gives rise to large electric field fluctuations, which in turn cause autoionization and ion dissociation. The fourth study analyzes several candidate order parameters as the basis for a Gaussian field theory of ion solvation. Finally, the fifth study discusses the most popular current explanation for observed charge asymmetry at liquid-vapor interfaces. This explanation, based on linear response of the surface polarization to the presence of an ion, is incorrect. Instead, the surface polarization responds non-linearly to the presence of an ion. Incorporating these non-linear fluctuations is essential to predict solvation free energies.

  12. Convergence and fluctuations of Regularized Tyler estimators

    KAUST Repository

    Kammoun, Abla

    2015-10-26

    This article studies the behavior of regularized Tyler estimators (RTEs) of scatter matrices. The key advantages of these estimators are twofold. First, they guarantee by construction a good conditioning of the estimate and second, being a derivative of robust Tyler estimators, they inherit their robustness properties, notably their resilience to the presence of outliers. Nevertheless, one major problem that poses the use of RTEs in practice is represented by the question of setting the regularization parameter p. While a high value of p is likely to push all the eigenvalues away from zero, it comes at the cost of a larger bias with respect to the population covariance matrix. A deep understanding of the statistics of RTEs is essential to come up with appropriate choices for the regularization parameter. This is not an easy task and might be out of reach, unless one considers asymptotic regimes wherein the number of observations n and/or their size N increase together. First asymptotic results have recently been obtained under the assumption that N and n are large and commensurable. Interestingly, no results concerning the regime of n going to infinity with N fixed exist, even though the investigation of this assumption has usually predated the analysis of the most difficult N and n large case. This motivates our work. In particular, we prove in the present paper that the RTEs converge to a deterministic matrix when n → ∞ with N fixed, which is expressed as a function of the theoretical covariance matrix. We also derive the fluctuations of the RTEs around this deterministic matrix and establish that these fluctuations converge in distribution to a multivariate Gaussian distribution with zero mean and a covariance depending on the population covariance and the parameter.

  13. Redox pioneer:Professor Christine Helen Foyer.

    Science.gov (United States)

    Del Río, Luis A

    2011-10-15

    Dr. Christine Foyer (B.Sc. 1974; Ph.D. 1977) is recognized here as a Redox Pioneer because she has published an article on redox biology that has been cited more than 1000 times, 4 other articles that have been cited more than 500 times, and a further 32 articles that have been each cited more than 100 times. During her Ph.D. at the Kings College, University of London, United Kingdom, Dr. Foyer discovered that ascorbate and glutathione and enzymes linking NADPH, glutathione, and ascorbate are localized in isolated chloroplast preparations. These observations pioneered the discovery of the ascorbate-glutathione cycle, now known as Foyer-Halliwell-Asada pathway after the names of the three major contributors, a crucial mechanism for H(2)O(2) metabolism in both animals and plants. Dr. Foyer has made a very significant contribution to our current understanding of the crucial roles of ascorbate and glutathione in redox biology, particularly in relation to photosynthesis, respiration, and chloroplast and mitochondrial redox signaling networks. "My view is that science…is compulsive and you have to keep with it all the time and not get despondent when things do not work well. Being passionate about science is what carries you through the hard times so that it isn't so much work, as a hobby that you do for a living. It is the thrill of achieving a better understanding and finding real pleasure in putting new ideas together, explaining data and passing on knowledge that keeps you going no matter what!" --Prof. Christine Helen Foyer.

  14. Chronopotentiometric determination of redox states of peptides

    Czech Academy of Sciences Publication Activity Database

    Dorčák, Vlastimil; Paleček, Emil

    2007-01-01

    Roč. 19, č. 23 (2007), s. 2405-2412 ISSN 1040-0397 R&D Projects: GA AV ČR(CZ) IAA500040513; GA ČR(CZ) GA301/07/0490; GA MŠk(CZ) LC06035 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : peptide redox states * constant current chronopotentiometry * catalytic hydrogen evolution Subject RIV: BO - Biophysics Impact factor: 2.949, year: 2007

  15. Electrochemical determination of thioredoxin redox states

    Czech Academy of Sciences Publication Activity Database

    Dorčák, Vlastimil; Paleček, Emil

    2009-01-01

    Roč. 81, č. 4 (2009), s. 1543-1548 ISSN 0003-2700 R&D Projects: GA AV ČR(CZ) KAN400310651; GA ČR(CZ) GA301/07/0490; GA MŠk(CZ) LC06035 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : thioredoxin redox states * constant current chronopotentiometric stripping * carbon and mercury electrodes Subject RIV: BO - Biophysics Impact factor: 5.214, year: 2009

  16. Electronic Connection Between the Quinone and Cytochrome c Redox Pools and Its Role in Regulation of Mitochondrial Electron Transport and Redox Signaling

    Science.gov (United States)

    Sarewicz, Marcin; Osyczka, Artur

    2015-01-01

    Mitochondrial respiration, an important bioenergetic process, relies on operation of four membranous enzymatic complexes linked functionally by mobile, freely diffusible elements: quinone molecules in the membrane and water-soluble cytochromes c in the intermembrane space. One of the mitochondrial complexes, complex III (cytochrome bc1 or ubiquinol:cytochrome c oxidoreductase), provides an electronic connection between these two diffusible redox pools linking in a fully reversible manner two-electron quinone oxidation/reduction with one-electron cytochrome c reduction/oxidation. Several features of this homodimeric enzyme implicate that in addition to its well-defined function of contributing to generation of proton-motive force, cytochrome bc1 may be a physiologically important point of regulation of electron flow acting as a sensor of the redox state of mitochondria that actively responds to changes in bioenergetic conditions. These features include the following: the opposing redox reactions at quinone catalytic sites located on the opposite sides of the membrane, the inter-monomer electronic connection that functionally links four quinone binding sites of a dimer into an H-shaped electron transfer system, as well as the potential to generate superoxide and release it to the intermembrane space where it can be engaged in redox signaling pathways. Here we highlight recent advances in understanding how cytochrome bc1 may accomplish this regulatory physiological function, what is known and remains unknown about catalytic and side reactions within the quinone binding sites and electron transfers through the cofactor chains connecting those sites with the substrate redox pools. We also discuss the developed molecular mechanisms in the context of physiology of mitochondria. PMID:25540143

  17. Relating hyporheic fluxes, residence times, and redox-sensitive biogeochemical processes upstream of beaver dams

    Science.gov (United States)

    Briggs, Martin A.; Lautz, Laura; Hare, Danielle K.

    2013-01-01

    Abstract. Small dams enhance the development of patchy microenvironments along stream corridors by trapping sediment and creating complex streambed morphologies. This patchiness drives intricate hyporheic flux patterns that govern the exchange of O2 and redox-sensitive solutes between the water column and the stream bed. We used multiple tracer techniques, naturally occurring and injected, to evaluate hyporheic flow dynamics and associated biogeochemical cycling and microbial reactivity around 2 beaver dams in Wyoming (USA). High-resolution fiber-optic distributed temperature sensing was used to collect temperature data over 9 vertical streambed profiles and to generate comprehensive vertical flux maps using 1-dimensional (1-D) heat-transport modeling. Coincident with these locations, vertical profiles of hyporheic water were collected every week and analyzed for dissolved O2, pH, dissolved organic C, and several conservative and redox-sensitive solutes. In addition, hyporheic and net stream aerobic microbial reactivity were analyzed with a constant-rate injection of the biologically sensitive resazurin (Raz) smart tracer. The combined results revealed a heterogeneous system with rates of downwelling hyporheic flow organized by morphologic unit and tightly coupled to the redox conditions of the subsurface. Principal component analysis was used to summarize the variability of all redox-sensitive species, and results indicated that hyporheic water varied from oxic-stream-like to anoxic-reduced in direct response to the hydrodynamic conditions and associated residence times. The anaerobic transition threshold predicted by the mean O2 Damko

  18. Structural characteristics of the redox-sensing coiled coil in the voltage-gated H+ channel.

    Science.gov (United States)

    Fujiwara, Yuichiro; Takeshita, Kohei; Nakagawa, Atsushi; Okamura, Yasushi

    2013-06-21

    Oxidation is an important biochemical defense mechanism, but it also elicits toxicity; therefore, oxidation must be under strict control. In phagocytotic events in neutrophils, the voltage-gated H(+) (Hv) channel is a key regulator of the production of reactive oxygen species against invading bacteria. The cytoplasmic domain of the Hv channel forms a dimeric coiled coil underpinning a dimerized functional unit. Importantly, in the alignment of the coiled-coil core, a conserved cysteine residue forms a potential intersubunit disulfide bond. In this study, we solved the crystal structures of the coiled-coil domain in reduced, oxidized, and mutated (Cys → Ser) states. The crystal structures indicate that a pair of Cys residues forms an intersubunit disulfide bond dependent on the redox conditions. CD spectroscopy revealed that the disulfide bond increases the thermal stability of the coiled-coil protein. We also reveal that two thiol modifier molecules are able to bind to Cys in a redox-dependent manner without disruption of the dimeric coiled-coil assembly. Thus, the biochemical properties of the cytoplasmic coiled-coil domain in the Hv channel depend on the redox condition, which may play a role in redox sensing in the phagosome.

  19. Redox Regulation in Amyotrophic Lateral Sclerosis

    Directory of Open Access Journals (Sweden)

    Sonam Parakh

    2013-01-01

    Full Text Available Amyotrophic lateral sclerosis (ALS is a neurodegenerative disease that results from the death of upper and lower motor neurons. Due to a lack of effective treatment, it is imperative to understand the underlying mechanisms and processes involved in disease progression. Regulations in cellular reduction/oxidation (redox processes are being increasingly implicated in disease. Here we discuss the possible involvement of redox dysregulation in the pathophysiology of ALS, either as a cause of cellular abnormalities or a consequence. We focus on its possible role in oxidative stress, protein misfolding, glutamate excitotoxicity, lipid peroxidation and cholesterol esterification, mitochondrial dysfunction, impaired axonal transport and neurofilament aggregation, autophagic stress, and endoplasmic reticulum (ER stress. We also speculate that an ER chaperone protein disulphide isomerase (PDI could play a key role in this dysregulation. PDI is essential for normal protein folding by oxidation and reduction of disulphide bonds, and hence any disruption to this process may have consequences for motor neurons. Addressing the mechanism underlying redox regulation and dysregulation may therefore help to unravel the molecular mechanism involved in ALS.

  20. Assessment of macro-micro element accumulation capabilities of Elodea nuttallii under gradient redox statuses with elevated NH4-N concentrations

    OpenAIRE

    Zaman, Tanjeena; Asaeda, Takashi

    2014-01-01

    Aquatic plants often encounter various redox conditions in their natural environment. Elodea nuttallii (Planch.), a submerged aquatic macrophyte, has a flexile ability to use different nutrient sources from various environments. In the present study, Elodea nuttallii was subjected to various redox conditions (+400 mV to –180 mV) at both normal (2.5 ppm) and high (10 ppm) ammonium concentrations and evaluated for macro and micro element accumulation. A reduced environment was prepared by addin...

  1. Integral fluctuation theorems for stochastic resetting systems

    Science.gov (United States)

    Pal, Arnab; Rahav, Saar

    2017-12-01

    We study the stochastic thermodynamics of resetting systems. Violation of microreversibility means that the well-known derivations of fluctuations theorems break down for dynamics with resetting. Despite that we show that stochastic resetting systems satisfy two integral fluctuation theorems. The first is the Hatano-Sasa relation describing the transition between two steady states. The second integral fluctuation theorem involves a functional that includes both dynamical and thermodynamic contributions. We find that the second law-like inequality found by Fuchs et al. for resetting systems [Europhys. Lett. 113, 60009 (2016), 10.1209/0295-5075/113/60009] can be recovered from this integral fluctuation theorem with the help of Jensen's inequality.

  2. Fluctuations in percolation of sparse complex networks

    Science.gov (United States)

    Bianconi, Ginestra

    2017-07-01

    We study the role of fluctuations in percolation of sparse complex networks. To this end we consider two random correlated realizations of the initial damage of the nodes and we evaluate the fraction of nodes that are expected to remain in the giant component of the network in both cases or just in one case. Our framework includes a message-passing algorithm able to predict the fluctuations in a single network, and an analytic prediction of the expected fluctuations in ensembles of sparse networks. This approach is applied to real ecological and infrastructure networks and it is shown to characterize the expected fluctuations in their response to external damage.

  3. Neutrino propagation in a fluctuating sun

    International Nuclear Information System (INIS)

    Burgess, C.P.; Michaud, D.

    1997-01-01

    We adapt to neutrino physics a general formulation for particle propagation in fluctuating media, initially developed for applications to electromagnetism and neutron optics. In leading approximation this formalism leads to the usual MSW effective Hamiltonian governing neutrino propagation through a medium. Next-to-leading contributions describe deviations from this description, which arise due to neutrino interactions with fluctuations in the medium. We compute these corrections for two types of fluctuations: (i) microscopic thermal fluctuations and (ii) macroscopic fluctuations in the medium s density. While the first of these reproduces standard estimates, which are negligible for applications to solar neutrinos, we find that the second can be quite large, since it grows in size with the correlation length of the fluctuation. We consider two models in some detail. For fluctuations whose correlations extend only over a local region in space of length l, appreciable effects for MSW oscillations arise if (δn/n) 2 l approx-gt 100m or so. Alternatively, a crude model of helioseismic p-waves gives appreciable effects only when (δn/n)approx-gt 1%. In general the dominant effect is to diminish the quality of the resonance, making the suppression of the 7 Be neutrinos a good experimental probe of fluctuations deep within the sun. Fluctuations can also provide a new mechanism for reducing the solar neutrino flux, giving an energy-independent suppression factor of 1/2 away from the resonant region, even for small vacuum mixing angles. copyright 1997 Academic Press, Inc

  4. Quantum fluctuations within the fragmentation theory

    International Nuclear Information System (INIS)

    Maruhn, J.A.; Hahn, J.; Lustig, H.J.; Zeigenhain, K.H.; Greiner, W.

    1980-01-01

    The measured spread of the fragment mass distributions in heavy ion collisions may be due to two quite different physical mechanisms: the quantum-mechanical uncertainty associated with collective motion in the mass asymmetry degree of freedom, and the spread caused by thermal excitation of the nuclear system. The fluctuations in physical observables induced in these ways are referred to as quantum fluctuations and statistical fluctuations. In this lecture quantum fluctuations are studied within the fragmentation theory. Mass distributions for spontaneous fission and low energy heavy ion collisions are investigated. (author)

  5. Pressure fluctuations on the bed of surge tank at the H.P. Zimapan, Hgo., with different arrangements studied on hydraulic model, with the lowest operation conditions; Fluctuaciones de presion en la base del pozo de oscilacion del P.H. Zimapan Hgo., con diferentes arreglos estudiados en modelo hidraulico ante las condiciones minimas de operacion

    Energy Technology Data Exchange (ETDEWEB)

    Marengo Mogollon, H. [Facultad de Ingenieria, Universidad Nacional Autonoma de Mexico (Mexico)]. E-mail: humberto.marengo@cfe.gob.mx; Ochoa Alvarez, F.J.; Cortes Cortes, C. [Comision Federal de Electricidad (Mexico)]. E-mail: federico.ochoa@cfe.gob.mx; carlos.cortes01@cfe.gob.mx

    2009-10-15

    In this paper, the pressure fluctuations of the surge tank in the Zimapan Hydroelectric Project are compared in a hydraulic model. The shaft is located lateral, over the conduction tunnel and in the simple form (permitting the tunnel entering the shaft); with and without orifice plates taking into account the demand and supply condition of energy with the minimum level of water of the conduction. It was determined the hydraulic efficiency and it was found that it was the best constructive option. [Spanish] En este articulo se comparan las fluctuaciones de presion en el pozo de oscilacion del P.H. Zimapan, Hgo., Mexico las cuales fueron estudiadas en modelo hidraulico al considerar dicho pozo ubicado en diferentes posiciones; lateralmente y sobre el eje de la conduccion; pozo simple y con tuberia de conexion; con y sin placa de orificio, para maniobras de rechazo y demanda de carga de las turbinas de generacion con el nivel del agua correspondiente al NAMINO. Se determino la eficiencia hidraulica comparandola con las otras opciones encontrandose que la mejor opcion para el funcionamiento hidraulico es el pozo lateral, que ademas permite optimizar el procedimiento constructivo.

  6. Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster

    Directory of Open Access Journals (Sweden)

    Katja E. Menger

    2015-06-01

    Full Text Available Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT, to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster.

  7. Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster

    Science.gov (United States)

    Menger, Katja E.; James, Andrew M.; Cochemé, Helena M.; Harbour, Michael E.; Chouchani, Edward T.; Ding, Shujing; Fearnley, Ian M.; Partridge, Linda; Murphy, Michael P.

    2015-01-01

    Summary Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster. PMID:26095360

  8. Mapping the diatom redox-sensitive proteome provides insight into response to nitrogen stress in the marine environment.

    Science.gov (United States)

    Rosenwasser, Shilo; Graff van Creveld, Shiri; Schatz, Daniella; Malitsky, Sergey; Tzfadia, Oren; Aharoni, Asaph; Levin, Yishai; Gabashvili, Alexandra; Feldmesser, Ester; Vardi, Assaf

    2014-02-18

    Diatoms are ubiquitous marine photosynthetic eukaryotes responsible for approximately 20% of global photosynthesis. Little is known about the redox-based mechanisms that mediate diatom sensing and acclimation to environmental stress. Here we used a quantitative mass spectrometry-based approach to elucidate the redox-sensitive signaling network (redoxome) mediating the response of diatoms to oxidative stress. We quantified the degree of oxidation of 3,845 cysteines in the Phaeodactylum tricornutum proteome and identified approximately 300 redox-sensitive proteins. Intriguingly, we found redox-sensitive thiols in numerous enzymes composing the nitrogen assimilation pathway and the recently discovered diatom urea cycle. In agreement with this finding, the flux from nitrate into glutamine and glutamate, measured by the incorporation of (15)N, was strongly inhibited under oxidative stress conditions. Furthermore, by targeting the redox-sensitive GFP sensor to various subcellular localizations, we mapped organelle-specific oxidation patterns in response to variations in nitrogen quota and quality. We propose that redox regulation of nitrogen metabolism allows rapid metabolic plasticity to ensure cellular homeostasis, and thus is essential for the ecological success of diatoms in the marine ecosystem.

  9. Redox stratification of an ancient lake in Gale crater, Mars.

    Science.gov (United States)

    Hurowitz, J A; Grotzinger, J P; Fischer, W W; McLennan, S M; Milliken, R E; Stein, N; Vasavada, A R; Blake, D F; Dehouck, E; Eigenbrode, J L; Fairén, A G; Frydenvang, J; Gellert, R; Grant, J A; Gupta, S; Herkenhoff, K E; Ming, D W; Rampe, E B; Schmidt, M E; Siebach, K L; Stack-Morgan, K; Sumner, D Y; Wiens, R C

    2017-06-02

    In 2012, NASA's Curiosity rover landed on Mars to assess its potential as a habitat for past life and investigate the paleoclimate record preserved by sedimentary rocks inside the ~150-kilometer-diameter Gale impact crater. Geological reconstructions from Curiosity rover data have revealed an ancient, habitable lake environment fed by rivers draining into the crater. We synthesize geochemical and mineralogical data from lake-bed mudstones collected during the first 1300 martian solar days of rover operations in Gale. We present evidence for lake redox stratification, established by depth-dependent variations in atmospheric oxidant and dissolved-solute concentrations. Paleoclimate proxy data indicate that a transition from colder to warmer climate conditions is preserved in the stratigraphy. Finally, a late phase of geochemical modification by saline fluids is recognized. Copyright © 2017, American Association for the Advancement of Science.

  10. Redox stratification of an ancient lake in Gale crater, Mars

    Science.gov (United States)

    Hurowitz, J. A.; Grotzinger, J. P.; Fischer, W. W.; McLennan, S. M.; Milliken, R. E.; Stein, N.; Vasavada, A. R.; Blake, D. F.; Dehouck, E.; Eigenbrode, J. L.; Fairén, A. G.; Frydenvang, J.; Gellert, R.; Grant, J. A.; Gupta, S.; Herkenhoff, K. E.; Ming, D. W.; Rampe, E. B.; Schmidt, M. E.; Siebach, K. L.; Stack-Morgan, K.; Sumner, D. Y.; Wiens, R. C.

    2017-06-01

    In 2012, NASA's Curiosity rover landed on Mars to assess its potential as a habitat for past life and investigate the paleoclimate record preserved by sedimentary rocks inside the ~150-kilometer-diameter Gale impact crater. Geological reconstructions from Curiosity rover data have revealed an ancient, habitable lake environment fed by rivers draining into the crater. We synthesize geochemical and mineralogical data from lake-bed mudstones collected during the first 1300 martian solar days of rover operations in Gale. We present evidence for lake redox stratification, established by depth-dependent variations in atmospheric oxidant and dissolved-solute concentrations. Paleoclimate proxy data indicate that a transition from colder to warmer climate conditions is preserved in the stratigraphy. Finally, a late phase of geochemical modification by saline fluids is recognized.

  11. Filling the gap: using non-invasive geophysical methods to monitor the processes leading to enhanced carbon turnover induced by periodic water table fluctuations

    Science.gov (United States)

    Mellage, A.; Pronk, G.; Atekwana, E. A.; Furman, A.; Rezanezhad, F.; Van Cappellen, P.

    2017-12-01

    Subsurface transition environments such as the capillary fringe are characterized by steep gradients in redox conditions. Spatial and temporal variations in electron acceptor and donor availability - driven by hydrological changes - may enhance carbon turnover, in some cases resulting in pulses of CO2-respiration. Filling the mechanistic knowledge gap between the hydrological driver and its biogeochemical effects hinges on our ability to monitor microbial activity and key geochemical markers at a high spatial and temporal resolution. However, direct access to subsurface biogeochemical processes is logistically difficult, invasive and usually expensive. In-line, non-invasive geophysical techniques - Spectral Induced Polarization (SIP) and Electrodic Potential (EP), specifically - offer a comparatively inexpensive alternative and can provide data with high spatial and temporal resolution. The challenge lies in linking electrical responses to specific changes in biogeochemical processes. We conducted SIP and EP measurements on a soil column experiment where an artificial soil mixture was subjected to monthly drainage and imbibition cycles. SIP responses showed a clear dependence on redox zonation and microbial abundance. Temporally variable responses exhibited no direct moisture dependence suggesting that the measured responses recorded changes in microbial activity and coincided with the depth interval over which enhanced carbon turnover was observed. EP measurements detected the onset of sulfate mineralization and mapped its depth zonation. SIP and EP signals thus detected enhanced microbial activity within the water table fluctuation zone as well as the timing of the development of specific reactive processes. These findings can be used to relate measured electrical signals to specific reaction pathways and help inform reactive transport models, increasing their predictive capabilities.

  12. Ediacaran Marine Redox Heterogeneity and Early Animal Ecosystems

    Science.gov (United States)

    Li, Chao; Planavsky, Noah J.; Shi, Wei; Zhang, Zihu; Zhou, Chuanming; Cheng, Meng; Tarhan, Lidya G.; Luo, Genming; Xie, Shucheng

    2015-11-01

    Oxygenation has widely been viewed as a major factor driving the emergence and diversification of animals. However, links between early animal evolution and shifts in surface oxygen levels have largely been limited to extrapolation of paleoredox conditions reconstructed from unfossiliferous strata to settings in which contemporaneous fossils were preserved. Herein, we present a multi-proxy paleoredox study of late Ediacaran (ca. 560-551 Ma) shales hosting the Miaohe Konservat-Lagerstätte of South China and, for comparison, equivalent non-fossil-bearing shales at adjacent sections. For the fossiliferous strata at Miaohe there is geochemical evidence for anoxic conditions, but paleontological evidence for at least episodically oxic conditions. An oxygen-stressed environment is consistent with the low diversity and simple morphology of Miaohe Biota macrofossils. However, there is no evidence for euxinic (anoxic and sulphidic) conditions for the fossiliferous strata at Miaohe, in contrast to adjacent unfossiliferous sections. Our results indicate that Ediacaran marine redox chemistry was highly heterogeneous, even at the kilometre-scale. Therefore, our study provides direct—rather than inferred—evidence that anoxia played a role in shaping a landmark Ediacaran ecosystem. If the anoxic conditions characteristic of the studied sections were widespread in the late Neoproterozoic, environmental stress would have hindered the development of complex ecosystems.

  13. Sorption of redox-sensitive elements: critical analysis

    International Nuclear Information System (INIS)

    Strickert, R.G.

    1980-12-01

    The redox-sensitive elements (Tc, U, Np, Pu) discussed in this report are of interest to nuclear waste management due to their long-lived isotopes which have a potential radiotoxic effect on man. In their lower oxidation states these elements have been shown to be highly adsorbed by geologic materials occurring under reducing conditions. Experimental research conducted in recent years, especially through the Waste Isolation Safety Assessment Program (WISAP) and Waste/Rock Interaction Technology (WRIT) program, has provided extensive information on the mechanisms of retardation. In general, ion-exchange probably plays a minor role in the sorption behavior of cations of the above three actinide elements. Formation of anionic complexes of the oxidized states with common ligands (OH - , CO -- 3 ) is expected to reduce adsorption by ion exchange further. Pertechnetate also exhibits little ion-exchange sorption by geologic media. In the reduced (IV) state, all of the elements are highly charged and it appears that they form a very insoluble compound (oxide, hydroxide, etc.) or undergo coprecipitation or are incorporated into minerals. The exact nature of the insoluble compounds and the effect of temperature, pH, pe, other chemical species, and other parameters are currently being investigated. Oxidation states other than Tc (IV,VII), U(IV,VI), Np(IV,V), and Pu(IV,V) are probably not important for the geologic repository environment expected, but should be considered especially when extreme conditions exist (radiation, temperature, etc.). Various experimental techniques such as oxidation-state analysis of tracer-level isotopes, redox potential measurement and control, pH measurement, and solid phase identification have been used to categorize the behavior of the various valence states

  14. Do Amplitudes of Water Level Fluctuations Affect the Growth and Community Structure of Submerged Macrophytes?

    Science.gov (United States)

    Wang, Mo-Zhu; Liu, Zheng-Yuan; Luo, Fang-Li; Lei, Guang-Chun; Li, Hong-Li

    2016-01-01

    Submerged macrophytes are subjected to potential mechanical stresses associated with fluctuating water levels in natural conditions. However, few experimental studies have been conducted to further understand the effects of water level fluctuating amplitude on submerged macrophyte species and their assemblages or communities. We designed a controlled experiment to investigate the responses of three submerged macrophyte species (Hydrilla verticillata, Ceratophyllum demersum and Elodea nuttallii) and their combinations in communities to three amplitudes (static, ± 30 cm, ± 60 cm) of water level fluctuations. Results showed that water level fluctuating amplitude had little effects on the community performance and the three tested species responded differently. H. verticillata exhibited more growth in static water and it was negatively affected by either of the water level fluctuations amplitude, however, growth parameters of H. verticillata in two fluctuating water level treatments (i.e., ± 30 cm, ± 60 cm) were not significantly different. On the other hand, the growth of C. demersum was not significantly correlated with different amplitude treatments. However, it became more abundant when water levels fluctuated. E. nuttallii was inhibited by the two fluctuating water level treatments, and was less in growth parameters compared to the other species especially in water level fluctuating conditions. The inherent differences in the adaptive capabilities of the tested species indicate that C. demersum or other species with similar responses may be dominant species to restore submerged macrophyte communities with great fluctuating water levels. Otherwise, H. verticillata, E. nuttallii or other species with similar responses could be considered for constructing the community in static water conditions.

  15. Do Amplitudes of Water Level Fluctuations Affect the Growth and Community Structure of Submerged Macrophytes?

    Directory of Open Access Journals (Sweden)

    Mo-Zhu Wang

    Full Text Available Submerged macrophytes are subjected to potential mechanical stresses associated with fluctuating water levels in natural conditions. However, few experimental studies have been conducted to further understand the effects of water level fluctuating amplitude on submerged macrophyte species and their assemblages or communities. We designed a controlled experiment to investigate the responses of three submerged macrophyte species (Hydrilla verticillata, Ceratophyllum demersum and Elodea nuttallii and their combinations in communities to three amplitudes (static, ± 30 cm, ± 60 cm of water level fluctuations. Results showed that water level fluctuating amplitude had little effects on the community performance and the three tested species responded differently. H. verticillata exhibited more growth in static water and it was negatively affected by either of the water level fluctuations amplitude, however, growth parameters of H. verticillata in two fluctuating water level treatments (i.e., ± 30 cm, ± 60 cm were not significantly different. On the other hand, the growth of C. demersum was not significantly correlated with different amplitude treatments. However, it became more abundant when water levels fluctuated. E. nuttallii was inhibited by the two fluctuating water level treatments, and was less in growth parameters compared to the other species especially in water level fluctuating conditions. The inherent differences in the adaptive capabilities of the tested species indicate that C. demersum or other species with similar responses may be dominant species to restore submerged macrophyte communities with great fluctuating water levels. Otherwise, H. verticillata, E. nuttallii or other species with similar responses could be considered for constructing the community in static water conditions.

  16. Fluctuations of offshore wind generation: Statistical modelling

    DEFF Research Database (Denmark)

    Pinson, Pierre; Christensen, Lasse E.A.; Madsen, Henrik

    2007-01-01

    The magnitude of power fluctuations at large offshore wind farms has a significant impact on the control and management strategies of their power output. If focusing on the minute scale, one observes successive periods with smaller and larger power fluctuations. It seems that different regimes yi...

  17. Dynamical interplay between fluctuations, electric fields and ...

    Indian Academy of Sciences (India)

    interplay between fluctuation in gradients, turbulent transport and radial electric fields has shown that these parameters ... electric fields and density fluctuations, ΓE¢B(t) = ˜n(t) ˜Eθ (t)/B. The poloidal electric field has been .... transport increases and the system performs a relaxation which tends to drive the plasma back to the ...

  18. Computer simulations of phospholipid - membrane thermodynamic fluctuations

    DEFF Research Database (Denmark)

    Pedersen, U.R.; Peters, Günther H.j.; Schröder, T.B.

    2008-01-01

    This paper reports all-atom computer simulations of five phospholipid membranes, DMPC, DPPC, DMPG, DMPS, and DMPSH, with a focus on the thermal equilibrium fluctuations of volume, energy, area, thickness, and order parameter. For the slow fluctuations at constant temperature and pressure (defined...

  19. temperature fluctuation inside inert atmosphere silos

    African Journals Online (AJOL)

    This research was conducted to study temperature fluctuation inside the inert atmosphere silos loaded with wheat, compare the temperature fluctuation across the top, middle and bottom part of the silo in relation to the ambient temperature. Temperature readings of the ambient and at the top, middle and bottom part of the ...

  20. Discharge Fluctuation Effect on Meandering River Bed Evolution

    Science.gov (United States)

    Kuntjoro; Saud, Ismail; Harijanto, Didik

    2017-11-01

    This research was based on some considerations: first discharge fluctuation argued that none rivers with constant discharge and second meandering river bed evolution with considering that none of rivers in a steady state without bed change. This research developed to get formulation the relationship between fluctuations discharge with the evolution of the bottom of river considering discharge, parameter rivers and parameter sediment. In the span of daily discharge data 1997-2011 and cross section monitoring annual results 1997-2011 evolution of bottom of a river subjects obtained: Formula 1 is the relationship between discharge fluctuations with rate of sedimentation (S) and Formula 2 is the relationship between discharge fluctuations with rate of erosion (E). Thus formula have higher prediction accuracy than other published formulas and it is applicable to predict Brantas River bed evolution approximate with the real conditions. Further analysis from the output KUN-QArSHOV formula produces: Erosion equation ΔS = 25,167e0,0034 ΔQ , on constant discharge, sedimentation value occur is 25.167.10-5 meter. Sedimentation equation ΔE = 8,3455e0,0075 ΔQ , on constant discharge, erosion value occure is 8.3455.10-5 meter. Critical point between sedimentation and erosion happened on discharge between 269 m3/second and 270 m3/second

  1. The effect of climate fluctuation on chimpanzee birth sex ratio.

    Directory of Open Access Journals (Sweden)

    Hjalmar S Kühl

    Full Text Available Climate and weather conditions, such as the North Atlantic Oscillation, precipitation and temperature influence the birth sex ratio (BSR of various higher latitude species, including deer, elephant seals or northern human populations. Although, tropical regions show only little variation in temperature, climate and weather conditions can fluctuate with consequences for phenology and food resource availability. Here, we evaluate, whether the BSR of chimpanzees, inhabiting African tropical forests, is affected by climate fluctuations as well. Additionally, we evaluate, if variation in consumption of a key food resource with high nutritional value, Coula edulis nuts, is linked to both climate fluctuations and variation in BSR. We use long-term data from two study groups located in Taï National Park, Côte d'Ivoire to assess the influence of local weather conditions and the global climate driver El Niño Southern Oscillation (ENSO on offspring sex. Côte d'Ivoire has experienced considerable climate variation over the last decades, with increasing temperature and declining precipitation. For both groups we find very similar time windows around the month of conception, in which offspring sex is well predicted by ENSO, with more males following low ENSO values, corresponding to periods of high rainfall. Furthermore, we find that the time spent cracking and feeding on Coula nuts is strongly influenced by climate conditions. Although, some of our analysis suggest that a higher proportion of males is born after periods with higher nut consumption frequency, we cannot conclude decisively at this point that nut consumption may influence shifts in BSR. All results combined suggest that also chimpanzees may experience climate related shifts in offspring sex ratios as response to climate fluctuation.

  2. On effective cross section fluctuations in unitary theory

    International Nuclear Information System (INIS)

    Lyuboshits, V.L.; Podgoretskij, M.I.

    1975-01-01

    In the framework of a single-channel theory of overlapping resonances, satisfying the unitarity condition, there were studied the fluctuations of the effective cross sections and the structure of the correlation coefficients for the amplitudes and cross sections. It is shown that at a high overlapping of the resonance levels the characteristic correlation period is determined by an average distance between the levels, but not by the width. The obtained results differ from the conclusions of the statistical Erikson theory

  3. Reducing number fluctuations of ultracold atomic gases via dispersive interrogation

    OpenAIRE

    Sawyer, Bianca J.; Deb, Amita B.; McKellar, Thomas; Kjærgaard, Niels

    2012-01-01

    We have used nondestructive laser probing to follow the central density evolution of a trapped atomic cloud during forced evaporative cooling. This was achieved in a heterodyne dispersive detection scheme. We propose to use this as a precursor measurement for predicting the atom number subsequent to evaporation and provide a simple experimental demonstration of the principle leading to a conditional reduction of classical number fluctuations.

  4. Coupled Quantum Fluctuations and Quantum Annealing

    Science.gov (United States)

    Hormozi, Layla; Kerman, Jamie

    We study the relative effectiveness of coupled quantum fluctuations, compared to single spin fluctuations, in the performance of quantum annealing. We focus on problem Hamiltonians resembling the the Sherrington-Kirkpatrick model of Ising spin glass and compare the effectiveness of different types of fluctuations by numerically calculating the relative success probabilities and residual energies in fully-connected spin systems. We find that for a small class of instances coupled fluctuations can provide improvement over single spin fluctuations and analyze the properties of the corresponding class. Disclaimer: This research was funded by ODNI, IARPA via MIT Lincoln Laboratory under Air Force Contract No. FA8721-05-C-0002. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of ODNI, IARPA, or the US Government.

  5. Multiplicity Distributions and Charged-neutral Fluctuations

    CERN Document Server

    Nayak, Tapan K.; Agnihotri, A.; Ahammed, Z.; Angelis, A.L.S.; Antonenko, V.; Arefev, V.; Astakhov, V.; Avdeitchikov, V.; Awes, T.C.; Baba, P.V.K.S.; Badyal, S.K.; Baldine, A.; Barabach, L.; Barlag, C.; Bathe, S.; Batiounia, B.; Bernier, T.; Bhalla, K.B.; Bhatia, V.S.; Blume, C.; Bock, R.; Bohne, E.M.; Bucher, D.; Buijs, A.; Buis, E.J.; Busching, H.; Carlen, L.; Chalyshev, V.; Chattopadhyay, S.; Chenawi, K.E.; Cherbatchev, R.; Chujo, T.; Claussen, A.; Das, A.C.; Decowski, M.P.; Djordjadze, V.; Donni, P.; Doubovik, I.; Dubey, A.K.; Dutta Majumda, M.R.; Eliseev, S.; Enosawa, K.; Feldmann, H.; Foka, P.; Fokin, S.; Frolov, V.; Ganti, M.S.; Garpman, S.; Gavrishchuk, O.; Geurts, F.J.M.; Ghosh, T.K.; Glasow, R.; Gupta, S.K.; Guskov, B.; Gustafsson, H.A.; Gutbrod, H.H.; Higuchi, R.; Hrivnacova, I.; Ippolitov, M.; Kalechofsky, H.; Kamermans, R.; Kampert, K.H.; Karadjev, K.; Karpio, K.; Kato, S.; Kees, S.; Kim, H.; Kolb, B.W.; Kosarev, I.; Koutcheryaev, I.; Kugler, A.; Kulinich, P.; Kumar, V.; Kurata, M.; Kurita, K.; Kuzmin, N.; Langbein, I.; Lebedev, A.; Lee, Y.Y.; Lohner, H.; Mahapatra, D.P.; Manko, V.; Martin, M.; Maximov, A.; Mehdiyev, Rashid R.; Mgebrichvili, G.; Miake, Y.; Mikhalev, D.; Mishra, G.C.; Miyamoto, Y.; Mohanty, B.; Morrison, Douglas R.O.; Mukhopadhyay, D.S.; Myalkovski, V.; Naef, H.; Nandi, B.K.; Nayak, S.K.; Nayak, T.K.; Neumaier, S.; Nianine, A.; Nikitine, V.; Nikolaev, S.; Nishimura, S.; Nomokov, P.; Nystrand, J.; Obenshain, F.E.; Oskarsson, A.; Otterlund, I.; Pachr, M.; Parfenov, A.; Pavliouk, S.; Peitzmann, T.; Petracek, V.; Plasil, F.; Purschke, M.L.; Raeven, B.; Rak, J.; Raniwala, R.; Raniwala, S.; Ramamurthy, V.S.; Rao, N.K.; Retiere, F.; Reygers, K.; Roland, G.; Rosselet, L.; Roufanov, I.; Rubio, J.M.; Sambyal, S.S.; Santo, R.; Sato, S.; Schlagheck, H.; Schmidt, H.R.; Shabratova, G.; Sibiriak, I.; Siemiarczuk, T.; Sinha, B.C.; Slavine, N.; Soderstrom, K.; Solomey, N.; Sood, G.; Sorensen, S.P.; Stankus, P.; Stefanek, G.; Steinberg, P.; Stenlund, E.; Stuken, D.; Sumbera, M.; Svensson, T.; Trivedi, M.D.; Tsvetkov, A.; Twenhofel, C.; Tykarski, L.; Urbahn, J.; van Eijndhoven, N.; van Heeringen, W.H.; van Nieuwenhuizen, G.J.; Vinogradov, A.; Viyogi, Y.P.; Vodopianov, A.S.; Voros, S.; Vos, M.A.; Wyslouch, B.; Yagi, K.; Yokota, Y.; Young, G.R.; Nayak, Tapan K.

    2001-01-01

    Results from the multiplicity distributions of inclusive photons and charged particles, scaling of particle multiplicities, event-by-event multiplicity fluctuations, and charged-neutral fluctuations in 158$\\cdot A$ GeV Pb+Pb collisions are presented and discussed. A scaling of charged particle multiplicity as $N_{part}^{1.07\\pm 0.05}$ and photons as $N_{part}^{1.12\\pm 0.03}$ have been observed, indicating violation of naive wounded nucleon model. The analysis of localized charged-neutral fluctuation indicates a model-independent demonstration of non-statistical fluctuations in both charged particles and photons in limited azimuthal regions. However, no correlated charged-neutral fluctuations are observed.

  6. Understanding controls on redox processes in floodplain sediments of the Upper Colorado River Basin

    Energy Technology Data Exchange (ETDEWEB)

    Noël, Vincent; Boye, Kristin; Kukkadapu, Ravi K.; Bone, Sharon; Lezama Pacheco, Juan S.; Cardarelli, Emily; Janot, Noémie; Fendorf, Scott; Williams, Kenneth H.; Bargar, John R.

    2017-12-15

    River floodplains, heavily used for water supplies, housing, agriculture, mining, and industry, may have water quality jeopardized by native or exogenous metals. Redox processes mediate the accumulation and release of these species in groundwater. Understanding the physicochemical, hydrological, and biogeochemical controls on the distribution and variability and variability of redox conditions is therefore critical to developing conceptual and numerical models of contaminants transport within floodplains. The distribution and intensity of redox activity at the Rifle, CO, site within the Upper Colorado River Basin (UCRB), are believed to be controlled by textural and compositional heterogeneities. Regionally, the UCRB is impacted by former uranium and vanadium ore processing, resulting in contaminations by U, Mo, V, As, Se, and Mn. Floodplains throughout the UCRB share sediment and groundwater characteristics, making redox activity regionally important to metal and radionuclide mobility. In this study, Fe and S speciation were used to track the distribution and stability of redox processes in sediment cores from three floodplain sites covering a 250 km range in the central portion of the UCRB. The results of the present study support the hypothesis that Fe(III) and sulfate reducing sediments are regionally important in the UCRB. The presence of organic carbon together with pore saturation were the key requirements for reducing conditions, dominated by sulfate-reduction. Sediment texture moderated the response of the system to external forcing, such as oxidant infusion, making fine-grain sediments resistant to change in comparison to coarser-grained sediments. Exposure to O2 and NO3- mediates the reactivity and longevity of freshly precipitated sulfides creating the potential for release of sequestered radionuclides and metals. The physical and chemical parameters of reducing zones evidenced in this study are thus thought to be key parameters on the dynamic exchange

  7. Sepiapterin Reductase Mediates Chemical Redox Cycling in Lung Epithelial Cells*

    Science.gov (United States)

    Yang, Shaojun; Jan, Yi-Hua; Gray, Joshua P.; Mishin, Vladimir; Heck, Diane E.; Laskin, Debra L.; Laskin, Jeffrey D.

    2013-01-01

    In the lung, chemical redox cycling generates highly toxic reactive oxygen species that can cause alveolar inflammation and damage to the epithelium, as well as fibrosis. In this study, we identified a cytosolic NADPH-dependent redox cycling activity in mouse lung epithelial cells as sepiapterin reductase (SPR), an enzyme important for the biosynthesis of tetrahydrobiopterin. Human SPR was cloned and characterized. In addition to reducing sepiapterin, SPR mediated chemical redox cycling of bipyridinium herbicides and various quinones; this activity was greatest for 1,2-naphthoquinone followed by 9,10-phenanthrenequinone, 1,4-naphthoquinone, menadione, and 2,3-dimethyl-1,4-naphthoquinone. Whereas redox cycling chemicals inhibited sepiapterin reduction, sepiapterin had no effect on redox cycling. Additionally, inhibitors such as dicoumarol, N-acetylserotonin, and indomethacin blocked sepiapterin reduction, with no effect on redox cycling. Non-redox cycling quinones, including benzoquinone and phenylquinone, were competitive inhibitors of sepiapterin reduction but noncompetitive redox cycling inhibitors. Site-directed mutagenesis of the SPR C-terminal substrate-binding site (D257H) completely inhibited sepiapterin reduction but had minimal effects on redox cycling. These data indicate that SPR-mediated reduction of sepiapterin and redox cycling occur by distinct mechanisms. The identification of SPR as a key enzyme mediating chemical redox cycling suggests that it may be important in generating cytotoxic reactive oxygen species in the lung. This activity, together with inhibition of sepiapterin reduction by redox-active chemicals and consequent deficiencies in tetrahydrobiopterin, may contribute to tissue injury. PMID:23640889

  8. Liquid Redox Electrolytes for Dye-Sensitized Solar Cells

    OpenAIRE

    Yu, Ze

    2012-01-01

    This thesis focuses on liquid redox electrolytes in dye-sensitized solar cells (DSCs). A liquid redox electrolyte, as one of the key constituents in DSCs, typically consists of a redox mediator, additives and a solvent. This thesis work concerns all these three aspects of liquid electrolytes, aiming through fundamental insights to enhance the photovoltaic performances of liquid DSCs. Initial attention has been paid to the iodine concentration effects in ionic liquid (IL)-based electrolytes. I...

  9. Stability and fluctuations in black hole thermodynamics

    International Nuclear Information System (INIS)

    Ruppeiner, George

    2007-01-01

    I examine thermodynamic fluctuations for a Kerr-Newman black hole in an extensive, infinite environment. This problem is not strictly solvable because full equilibrium with such an environment cannot be achieved by any black hole with mass M, angular momentum J, and charge Q. However, if we consider one (or two) of M, J, or Q to vary so slowly compared with the others that we can regard it as fixed, instances of stability occur, and thermodynamic fluctuation theory could plausibly apply. I examine seven cases with one, two, or three independent fluctuating variables. No knowledge about the thermodynamic behavior of the environment is needed. The thermodynamics of the black hole is sufficient. Let the fluctuation moment for a thermodynamic quantity X be √( 2 >). Fluctuations at fixed M are stable for all thermodynamic states, including that of a nonrotating and uncharged environment, corresponding to average values J=Q=0. Here, the fluctuation moments for J and Q take on maximum values. That for J is proportional to M. For the Planck mass it is 0.3990(ℎ/2π). That for Q is 3.301e, independent of M. In all cases, fluctuation moments for M, J, and Q go to zero at the limit of the physical regime, where the temperature goes to zero. With M fluctuating there are no stable cases for average J=Q=0. But, there are transitions to stability marked by infinite fluctuations. For purely M fluctuations, this coincides with a curve which Davies identified as a phase transition

  10. Extending roGFP Emission via Förster-Type Resonance Energy Transfer Relay Enables Simultaneous Dual Compartment Ratiometric Redox Imaging in Live Cells.

    Science.gov (United States)

    Norcross, Stevie; Trull, Keelan J; Snaider, Jordan; Doan, Sara; Tat, Kiet; Huang, Libai; Tantama, Mathew

    2017-11-22

    Reactive oxygen species (ROS) mediate both intercellular and intraorganellar signaling, and ROS propagate oxidative stress between cellular compartments such as mitochondria and the cytosol. Each cellular compartment contains its own sources of ROS as well as antioxidant mechanisms, which contribute to dynamic fluctuations in ROS levels that occur during signaling, metabolism, and stress. However, the coupling of redox dynamics between cellular compartments has not been well studied because of the lack of available sensors to simultaneously measure more than one subcellular compartment in the same cell. Currently, the redox-sensitive green fluorescent protein, roGFP, has been used extensively to study compartment-specific redox dynamics because it provides a quantitative ratiometric readout and it is amenable to subcellular targeting as a genetically encoded sensor. Here, we report a new family of genetically encoded fluorescent protein sensors that extend the fluorescence emission of roGFP via Förster-type resonance energy transfer to an acceptor red fluorescent protein for dual-color live-cell microscopy. We characterize the redox and optical properties of the sensor proteins, and we demonstrate that they can be used to simultaneously measure cytosolic and mitochondrial ROS in living cells. Furthermore, we use these sensors to reveal cell-to-cell heterogeneity in redox coupling between the cytosol and mitochondria when neuroblastoma cells are exposed to reductive and metabolic stresses.

  11. Factors Controlling Redox Speciation of Plutonium and Neptunium in Extraction Separation Processes

    Energy Technology Data Exchange (ETDEWEB)

    Paulenova, Alena [Principal Investigator; Vandegrift, III, George F. [Collaborator

    2013-09-24

    The objective of the project was to examine the factors controlling redox speciation of plutonium and neptunium in UREX+ extraction in terms of redox potentials, redox mechanism, kinetics and thermodynamics. Researchers employed redox-speciation extractions schemes in parallel to the spectroscopic experiments. The resulting distribution of redox species w studied uring spectroscopic, electrochemical, and spectro-electrochemical methods. This work reulted in collection of data on redox stability and distribution of redox couples in the nitric acid/nitrate electrolyte and the development of redox buffers to stabilize the desired oxidation state of separated radionuclides. The effects of temperature and concentrations on the redox behavior of neptunium were evaluated.

  12. Oxidative shift in tissue redox potential increases beat-to-beat variability of action potential duration.

    Science.gov (United States)

    Kistamás, Kornél; Hegyi, Bence; Váczi, Krisztina; Horváth, Balázs; Bányász, Tamás; Magyar, János; Szentandrássy, Norbert; Nánási, Péter P

    2015-07-01

    Profound changes in tissue redox potential occur in the heart under conditions of oxidative stress frequently associated with cardiac arrhythmias. Since beat-to-beat variability (short term variability, SV) of action potential duration (APD) is a good indicator of arrhythmia incidence, the aim of this work was to study the influence of redox changes on SV in isolated canine ventricular cardiomyocytes using a conventional microelectrode technique. The redox potential was shifted toward a reduced state using a reductive cocktail (containing dithiothreitol, glutathione, and ascorbic acid) while oxidative changes were initiated by superfusion with H2O2. Redox effects were evaluated as changes in "relative SV" determined by comparing SV changes with the concomitant APD changes. Exposure of myocytes to the reductive cocktail decreased SV significantly without any detectable effect on APD. Application of H2O2 increased both SV and APD, but the enhancement of SV was the greater, so relative SV increased. Longer exposure to H2O2 resulted in the development of early afterdepolarizations accompanied by tremendously increased SV. Pretreatment with the reductive cocktail prevented both elevation in relative SV and the development of afterdepolarizations. The results suggest that the increased beat-to-beat variability during an oxidative stress contributes to the generation of cardiac arrhythmias.

  13. S-Nitrosylation-Mediated Redox Transcriptional Switch Modulates Neurogenesis and Neuronal Cell Death

    Directory of Open Access Journals (Sweden)

    Shu-ichi Okamoto

    2014-07-01

    Full Text Available Redox-mediated posttranslational modifications represent a molecular switch that controls major mechanisms of cell function. Nitric oxide (NO can mediate redox reactions via S-nitrosylation, representing transfer of an NO group to a critical protein thiol. NO is known to modulate neurogenesis and neuronal survival in various brain regions in disparate neurodegenerative conditions. However, a unifying molecular mechanism linking these phenomena remains unknown. Here, we report that S-nitrosylation of myocyte enhancer factor 2 (MEF2 transcription factors acts as a redox switch to inhibit both neurogenesis and neuronal survival. Structure-based analysis reveals that MEF2 dimerization creates a pocket, facilitating S-nitrosylation at an evolutionally conserved cysteine residue in the DNA binding domain. S-Nitrosylation disrupts MEF2-DNA binding and transcriptional activity, leading to impaired neurogenesis and survival in vitro and in vivo. Our data define a molecular switch whereby redox-mediated posttranslational modification controls both neurogenesis and neurodegeneration via a single transcriptional signaling cascade.

  14. Biogeochemical redox processes and their impact on contaminant dynamics

    Science.gov (United States)

    Borch, Thomas; Kretzschmar, Ruben; Kappler, Andreas; Van Cappellen, Philippe; Ginder-Vogel, Matthew; Campbell, Kate M.

    2010-01-01

    Life and element cycling on Earth is directly related to electron transfer (or redox) reactions. An understanding of biogeochemical redox processes is crucial for predicting and protecting environmental health and can provide new opportunities for engineered remediation strategies. Energy can be released and stored by means of redox reactions via the oxidation of labile organic carbon or inorganic compounds (electron donors) by microorganisms coupled to the reduction of electron acceptors including humic substances, iron-bearing minerals, transition metals, metalloids, and actinides. Environmental redox processes play key roles in the formation and dissolution of mineral phases. Redox cycling of naturally occurring trace elements and their host minerals often controls the release or sequestration of inorganic contaminants. Redox processes control the chemical speciation, bioavailability, toxicity, and mobility of many major and trace elements including Fe, Mn, C, P, N, S, Cr, Cu, Co, As, Sb, Se, Hg, Tc, and U. Redox-active humic substances and mineral surfaces can catalyze the redox transformation and degradation of organic contaminants. In this review article, we highlight recent advances in our understanding of biogeochemical redox processes and their impact on contaminant fate and transport, including future research needs.

  15. Exercise redox biochemistry: Conceptual, methodological and technical recommendations

    Directory of Open Access Journals (Sweden)

    James N. Cobley

    2017-08-01

    Full Text Available Exercise redox biochemistry is of considerable interest owing to its translational value in health and disease. However, unaddressed conceptual, methodological and technical issues complicate attempts to unravel how exercise alters redox homeostasis in health and disease. Conceptual issues relate to misunderstandings that arise when the chemical heterogeneity of redox biology is disregarded: which often complicates attempts to use redox-active compounds and assess redox signalling. Further, that oxidised macromolecule adduct levels reflect formation and repair is seldom considered. Methodological and technical issues relate to the use of out-dated assays and/or inappropriate sample preparation techniques that confound biochemical redox analysis. After considering each of the aforementioned issues, we outline how each issue can be resolved and provide a unifying set of recommendations. We specifically recommend that investigators: consider chemical heterogeneity, use redox-active compounds judiciously, abandon flawed assays, carefully prepare samples and assay buffers, consider repair/metabolism, use multiple biomarkers to assess oxidative damage and redox signalling. Keywords: Exercise, Oxidative stress, Free radical, Antioxidants, Redox signalling

  16. Numerical analysis of temperature fluctuation in core outlet region of China experimental fast reactor

    International Nuclear Information System (INIS)

    Zhu Huanjun; Xu Yijun

    2014-01-01

    The temperature fluctuation in core outlet region of China Experimental Fast Reactor (CEFR) was numerically simulated by the CFD software Star CCM+. With the core outlet temperatures, flows etc. under rated conditions given as boundary conditions, a 1/4 region model of the reactor core outlet region was established and calculated using LES method for this problem. The analysis results show that while CEFR operates under rated conditions, the temperature fluctuation in lower part of core outlet region is mainly concentrated in area over the edge components (steel components, control rod assembly), and one in upper part is remarkable in area above all the components. The largest fluctuation amplitude is 19 K and the remarkable frequency is below 5 Hz, and it belongs to typically low frequency fluctuation. The conclusion is useful for further experimental work. (authors)

  17. Redox-mediated quorum sensing in plants.

    Science.gov (United States)

    Fuller, Alexandra W; Young, Phoebe; Pierce, B Daniel; Kitson-Finuff, Jamie; Jain, Purvi; Schneider, Karl; Lazar, Stephen; Taran, Olga; Palmer, Andrew G; Lynn, David G

    2017-01-01

    The rhizosphere, the narrow zone of soil around plant roots, is a complex network of interactions between plants, bacteria, and a variety of other organisms. The absolute dependence on host-derived signals, or xenognosins, to regulate critical developmental checkpoints for host commitment in the obligate parasitic plants provides a window into the rhizosphere's chemical dynamics. These sessile intruders use H2O2 in a process known as semagenesis to chemically modify the mature root surfaces of proximal host plants and generate p-benzoquinones (BQs). The resulting redox-active signaling network regulates the spatial and temporal commitments necessary for host attachment. Recent evidence from non-parasites, including Arabidopsis thaliana, establishes that reactive oxygen species (ROS) production regulates similar redox circuits related to root recognition, broadening xenognosins' role beyond the parasites. Here we compare responses to the xenognosin dimethoxybenzoquinone (DMBQ) between the parasitic plant Striga asiatica and the non-parasitic A. thaliana. Exposure to DMBQ simulates the proximity of a mature root surface, stimulating an increase in cytoplasmic Ca2+ concentration in both plants, but leads to remarkably different phenotypic responses in the parasite and non-parasite. In S. asiatica, DMBQ induces development of the host attachment organ, the haustorium, and decreases ROS production at the root tip, while in A. thaliana, ROS production increases and further growth of the root tip is arrested. Obstruction of Ca2+ channels and the addition of antioxidants both lead to a decrease in the DMBQ response in both parasitic and non-parasitic plants. These results are consistent with Ca2+ regulating the activity of NADPH oxidases, which in turn sustain the autocatalytic production of ROS via an external quinone/hydroquinone redox cycle. Mechanistically, this chemistry is similar to black and white photography with the emerging dynamic reaction-diffusion network

  18. Redox-mediated quorum sensing in plants.

    Directory of Open Access Journals (Sweden)

    Alexandra W Fuller

    Full Text Available The rhizosphere, the narrow zone of soil around plant roots, is a complex network of interactions between plants, bacteria, and a variety of other organisms. The absolute dependence on host-derived signals, or xenognosins, to regulate critical developmental checkpoints for host commitment in the obligate parasitic plants provides a window into the rhizosphere's chemical dynamics. These sessile intruders use H2O2 in a process known as semagenesis to chemically modify the mature root surfaces of proximal host plants and generate p-benzoquinones (BQs. The resulting redox-active signaling network regulates the spatial and temporal commitments necessary for host attachment. Recent evidence from non-parasites, including Arabidopsis thaliana, establishes that reactive oxygen species (ROS production regulates similar redox circuits related to root recognition, broadening xenognosins' role beyond the parasites. Here we compare responses to the xenognosin dimethoxybenzoquinone (DMBQ between the parasitic plant Striga asiatica and the non-parasitic A. thaliana. Exposure to DMBQ simulates the proximity of a mature root surface, stimulating an increase in cytoplasmic Ca2+ concentration in both plants, but leads to remarkably different phenotypic responses in the parasite and non-parasite. In S. asiatica, DMBQ induces development of the host attachment organ, the haustorium, and decreases ROS production at the root tip, while in A. thaliana, ROS production increases and further growth of the root tip is arrested. Obstruction of Ca2+ channels and the addition of antioxidants both lead to a decrease in the DMBQ response in both parasitic and non-parasitic plants. These results are consistent with Ca2+ regulating the activity of NADPH oxidases, which in turn sustain the autocatalytic production of ROS via an external quinone/hydroquinone redox cycle. Mechanistically, this chemistry is similar to black and white photography with the emerging dynamic reaction

  19. Relaxation of enthalpy fluctuations during sub-T(g) annealing of glassy selenium.

    Science.gov (United States)

    Gulbiten, Ozgur; Mauro, John C; Lucas, Pierre

    2013-06-28

    The relaxation behavior of glass is influenced by the presence of dynamical heterogeneities, which lead to an intrinsically non-monotonic decay of fluctuations in density and enthalpy during isothermal annealing. This is apparently a universal feature of fragile glass forming systems associated with localized spatial variations in relaxation time. Here we present direct experimental observation of the nonmonotonic evolution of enthalpy fluctuations in glassy selenium annealed near room temperature. The nonmonotonic change in the distribution of enthalpy fluctuations measured by heat capacity spectroscopy offers direct evidence for the presence of dynamical heterogeneity in this glass. An enthalpy landscape model of selenium is then used to simulate annealing under identical conditions. The simulation results closely follow the evolution of enthalpy fluctuations observed experimentally. The close match between model and experiment demonstrate that enthalpy and density fluctuations are sources of dynamical heterogeneities in glassy materials.

  20. Redox polymer mediation for enzymatic biofuel cells

    Science.gov (United States)

    Gallaway, Joshua

    Mediated biocatalytic cathodes prepared from the oxygen-reducing enzyme laccase and redox-conducting osmium hydrogels were characterized for use as cathodes in enzymatic biofuel cells. A series of osmium-based redox polymers was synthesized with redox potentials spanning the range from 0.11 V to 0.85 V (SHE), and the resulting biocatalytic electrodes were modeled to determine reaction kinetic constants using the current response, measured osmium concentration, and measured apparent electron diffusion. As in solution-phase systems, the bimolecular rate constant for mediation was found to vary greatly with mediator potential---from 250 s-1M-1 when mediator and enzyme were close in potential to 9.4 x 10 4 s-1M-1 when this overpotential was large. Optimum mediator potential for a cell operating with a non-limiting platinum anode and having no mass transport limitation from bulk solution was found to be 0.66 V (SHE). Redox polymers were synthesized under different concentrations, producing osmium variation. An increase from 6.6% to 7.2% osmium increased current response from 1.2 to 2.1 mA/cm2 for a planar film in 40°C oxygen-saturated pH 4 buffer, rotating at 900 rpm. These results translated to high surface area electrodes, nearly doubling current density to 13 mA/cm2, the highest to date for such an electrode. The typical fungal laccase from Trametes versicolor was replaced by a bacterially-expressed small laccase from Streptomyces coelicolor, resulting in biocatalytic films that reduced oxygen at increased pH, with full functionality at pH 7, producing 1.5 mA/cm 2 in planar configuration. Current response was biphasic with pH, matching the activity profile of the free enzyme in solution. The mediated enzyme electrode system was modeled with respect to apparent electron diffusion, mediator concentration, and transport of oxygen from bulk solution, all of which are to some extent controlled by design. Each factor was found to limit performance in certain circumstances

  1. Aqueous electrolytes for redox flow battery systems

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Tianbiao; Li, Bin; Wei, Xiaoliang; Nie, Zimin; Wang, Wei; Liu, Jun; Sprenkle, Vincent L.

    2017-10-17

    An aqueous redox flow battery system includes an aqueous catholyte and an aqueous anolyte. The aqueous catholyte may comprise (i) an optionally substituted thiourea or a nitroxyl radical compound and (ii) a catholyte aqueous supporting solution. The aqueous anolyte may comprise (i) metal cations or a viologen compound and (ii) an anolyte aqueous supporting solution. The catholyte aqueous supporting solution and the anolyte aqueous supporting solution independently may comprise (i) a proton source, (ii) a halide source, or (iii) a proton source and a halide source.

  2. Redox-active and Redox-silent Compounds: Synergistic Therapeutics in Cancer

    Czech Academy of Sciences Publication Activity Database

    Tomasetti, M.; Santarelli, L.; Alleva, R.; Dong, L.F.; Neužil, Jiří

    2015-01-01

    Roč. 22, č. 5 (2015), s. 552-568 ISSN 0929-8673 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0109 Institutional support: RVO:86652036 Keywords : Apoptosis * autophagy * redox-active agents Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.455, year: 2015

  3. Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae under Hyperosmotic Stress during Icewine Fermentation

    Directory of Open Access Journals (Sweden)

    Fei Yang

    2017-11-01

    Full Text Available Acetic acid is undesired in Icewine. It is unclear whether its production by fermenting yeast is linked to the nicotinamide adenine dinucleotide (NAD+/NADH system or the nicotinamide adenine dinucleotide phosphate (NADP+/NADPH system. To answer this question, the redox status of yeast cytosolic NAD(H and NADP(H were analyzed along with yeast metabolites to determine how redox status differs under Icewine versus table wine fermentation. Icewine juice and dilute Icewine juice were inoculated with commercial wine yeast Saccharomyces cerevisiae K1-V1116. Acetic acid was 14.3-fold higher in Icewine fermentation than the dilute juice condition. The ratio of NAD+ to total NAD(H was 24-fold higher in cells in Icewine fermentation than the ratio from the dilute juice condition. Conversely, the ratio of NADP+ to total NADP(H from the dilute fermentation was 2.9-fold higher than that in the Icewine condition. These results support the hypothesis that in Icewine, increased NAD+ triggered the catalysis of NAD+-dependent aldehyde dehydrogenase(s (Aldp(s, which led to the elevated level of acetic acid in Icewine, whereas, in the dilute condition, NADP+ triggered NADP+-dependent Aldp(s, resulting in a lower level of acetic acid. This work, for the first time, analyzed the yeast cytosolic redox status and its correlation to acetic acid production, providing a more comprehensive understanding of the mechanism of acetic acid production in Icewine.

  4. Role of oxbow lakes in controlling redox geochemistry of shallow groundwater under a heterogeneous fluvial sedimentary environment in an agricultural field: Coexistence of iron and sulfate reduction.

    Science.gov (United States)

    Choi, Byoung-Young; Yun, Seong-Taek; Kim, Kyoung-Ho

    2016-01-01

    This study aimed to extend the knowledge of the vertical distribution of redox conditions of shallow groundwater in heterogeneous fluvial sediments near oxbow lakes. For this study, we revisited the study area of Kim et al. (2009) to examine the redox zoning in details. Three multi-level samplers were installed along a flow path near two oxbow lakes to obtain vertical profiles of the subsurface geology and hydrochemical and isotopic data (δ(18)O and δD of water, δ(15)N and δ(18)O of nitrate, and δ(34)S of sulfate) of groundwater. Geologic logging showed that characteristics of the heterogeneous subsurface geology are closely related to the pattern of vertical redox zoning. Hydrochemical data in conjunction with nitrogen and sulfur isotope data show that the redox status of groundwater near oxbow lakes is controlled by denitrification, iron reduction, and sulfate reduction. The oxidizing condition of groundwater occurs in the sand-dominant alluvium located in the up-gradient of oxbow lakes, whereas the reducing condition accompanying denitrification, iron reduction, and local sulfate reduction is developed in silt-rich alluvium in and the downgradient of oxbow lakes. The occurrence of sulfate reduction was newly found in this study. However, the vertical profiles of redox-sensitive parameters show that iron reduction and sulfate reduction occur concurrently near oxbow lakes, although the measured redox potentials suggest that thermodynamic conditions are controlled by the stability of Fe(2+)/Fe-oxides. Therefore, this study shows that the redox condition of groundwater in the iron-rich zone should be carefully interpreted. For this purpose, depth-specific sampling and careful examination of sulfur isotope data will be very useful for identifying the redox processes occurring in the zone with overlapping iron reduction and sulfate reduction in heterogeneous fluvial sediments. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. The Measurement of Reversible Redox Dependent Post-translational Modifications and Their Regulation of Mitochondrial and Skeletal Muscle Function

    Energy Technology Data Exchange (ETDEWEB)

    Kramer, Philip A.; Duan, Jicheng; Qian, Wei-Jun; Marcinek, David J.

    2015-11-25

    Mitochondrial oxidative stress is a common feature of skeletal myopathies across multiple conditions; however, the mechanism by which it contributes to skeletal muscle dysfunction remains controversial. Oxidative damage to proteins, lipids, and DNA has received the most attention, yet an important role for reversible redox post-translational modifications (PTMs) in pathophysiology is emerging. The possibility that these PTMs can exert dynamic control of muscle function implicates them as a mechanism contributing to skeletal muscle dysfunction in chronic disease. Herein, we discuss the significance of thiol-based redox dependent modifications to mitochondrial, myofibrillar and excitation-contraction (EC) coupling proteins with an emphasis on how these changes could alter skeletal muscle performance under chronically stressed conditions. A major barrier to a better mechanistic understanding of the role of reversible redox PTMs in muscle function is the technical challenges associated with accurately measuring the changes of site-specific redox PTMs. Here we will critically review current approaches with an emphasis on sample preparation artifacts, quantitation, and specificity. Despite these challenges, the ability to accurately quantify reversible redox PTMs is critical to understanding the mechanisms by which mitochondrial oxidative stress contributes to skeletal muscle dysfunction in chronic diseases.

  6. The measurement of reversible redox dependent post-translational modifications and their regulation of mitochondrial and skeletal muscle function

    Directory of Open Access Journals (Sweden)

    Philip A Kramer

    2015-11-01

    Full Text Available Mitochondrial oxidative stress is a common feature of skeletal myopathies across multiple conditions; however, the mechanism by which it contributes to skeletal muscle dysfunction remains controversial. Oxidative damage to proteins, lipids, and DNA has received the most attention, yet an important role for reversible redox post-translational modifications (PTMs in pathophysiology is emerging. The possibility that these PTMs can exert dynamic control of muscle function implicates them as a mechanism contributing to skeletal muscle dysfunction in chronic disease. Herein, we discuss the significance of thiol-based redox dependent modifications to mitochondrial, myofibrillar and excitation-contraction (EC coupling proteins with an emphasis on how these changes could alter skeletal muscle performance under chronically stressed conditions. A major barrier to a better mechanistic understanding of the role of reversible redox PTMs in muscle function is the technical challenges associated with accurately measuring the changes of site-specific redox PTMs. Here we will critically review current approaches with an emphasis on sample preparation artifacts, quantitation, and specificity. Despite these challenges, the ability to accurately quantify reversible redox PTMs is critical to understanding the mechanisms by which mitochondrial oxidative stress contributes to skeletal muscle dysfunction in chronic diseases.

  7. A METHOD FOR DETERMINING LOCATION OF VOLTAGE FLUCTUATIONS SOURCE IN ELECTRIC GRID

    Directory of Open Access Journals (Sweden)

    G.A. Senderovich

    2016-06-01

    Full Text Available Purpose. The purpose of work is development of a method of definition of the location of a source of fluctuations of voltage. Methodology. The reasons of emergence of fluctuations of voltage at an arrangement of a source both in power lines, and in the consumer's networks, are connected with changes of consumption and active and reactive capacities. As criterion for definition of the location of a source of fluctuations of voltage we choose change of size of the active power received by reception substation on equivalent communication with system. The source of fluctuations of voltage is external for the consumer if emergence of fluctuations of voltage leads to the coordinated changes of tension and consumed in the area of active power that corresponds to a condition of the positive regulating effect of active loading on voltage (1. The source of fluctuations of voltage is internal for the consumer if emergence of fluctuations of voltage leads to counter changes of tension and consumed in the area of active power that resembles a condition of the negative regulating effect of active loading on voltage superficially (6. Results. The method of definition of the location of a source of fluctuations of voltage in an electric network which, works by the principle of an assessment of correlation of change of power and tension in a power supply network is developed. The method allows to consider shift between extrema of curves of change of voltage of U(t and power of Pload(t. Originality. The method of definition of an arrangement of a source of fluctuations of voltage is developed. Practical value. The answer to this question where the source of fluctuations of voltage (in the territory of the consumer is located or in an external network confirmed with the determined calculation, can form a basis of the expert opinion for the solution of legal disputes at an assessment of the damages caused by poor quality of electric energy.

  8. Quantum fluctuation theorems and power measurements

    International Nuclear Information System (INIS)

    Prasanna Venkatesh, B; Watanabe, Gentaro; Talkner, Peter

    2015-01-01

    Work in the paradigm of the quantum fluctuation theorems of Crooks and Jarzynski is determined by projective measurements of energy at the beginning and end of the force protocol. In analogy to classical systems, we consider an alternative definition of work given by the integral of the supplied power determined by integrating up the results of repeated measurements of the instantaneous power during the force protocol. We observe that such a definition of work, in spite of taking account of the process dependence, has different possible values and statistics from the work determined by the conventional two energy measurement approach (TEMA). In the limit of many projective measurements of power, the system’s dynamics is frozen in the power measurement basis due to the quantum Zeno effect leading to statistics only trivially dependent on the force protocol. In general the Jarzynski relation is not satisfied except for the case when the instantaneous power operator commutes with the total Hamiltonian at all times. We also consider properties of the joint statistics of power-based definition of work and TEMA work in protocols where both values are determined. This allows us to quantify their correlations. Relaxing the projective measurement condition, weak continuous measurements of power are considered within the stochastic master equation formalism. Even in this scenario the power-based work statistics is in general not able to reproduce qualitative features of the TEMA work statistics. (paper)

  9. Fluctuations and structure of amphiphilic films

    International Nuclear Information System (INIS)

    Gourier, CH.

    1996-01-01

    This thesis is divided in three parts.The first part exposes in a theoretical point of view, how the fluctuations spectrum of an amphiphilic film is governed by its properties and its bidimensional characteristics.The measurements of fluctuations spectra of an interface are accessible with the measurement of intensity that interface diffuses out of the specular angle, we present in the second chapter the principles of the X rays diffusion by a real interface and see how the diffuse diffusion experiments allow to determine the fluctuations spectrum of an amphiphilic film. The second part is devoted to the different experimental techniques that have allowed to realize the study of fluctuation as well as the structural study.The third part is devoted to experimental results concerning the measurements of fluctuations spectra and to the study of the structure of amphiphilic films. We show that it is possible by using an intense source of X rays (ESRF: European Synchrotron Radiation Facility) to measure the water and amphiphilic films fluctuations spectra until molecular scales. The last chapter is devoted to the structural study and film fluctuations made of di-acetylenic molecules. (N.C.)

  10. Fluctuating Asymmetry of Human Populations: A Review

    Directory of Open Access Journals (Sweden)

    John H. Graham

    2016-12-01

    Full Text Available Fluctuating asymmetry, the random deviation from perfect symmetry, is a widely used population-level index of developmental instability, developmental noise, and robustness. It reflects a population’s state of adaptation and genomic coadaptation. Here, we review the literature on fluctuating asymmetry of human populations. The most widely used bilateral traits include skeletal, dental, and facial dimensions; dermatoglyphic patterns and ridge counts; and facial shape. Each trait has its advantages and disadvantages, but results are most robust when multiple traits are combined into a composite index of fluctuating asymmetry (CFA. Both environmental (diet, climate, toxins and genetic (aneuploidy, heterozygosity, inbreeding stressors have been linked to population-level variation in fluctuating asymmetry. In general, these stressors increase average fluctuating asymmetry. Nevertheless, there have been many conflicting results, in part because (1 fluctuating asymmetry is a weak signal in a sea of noise; and (2 studies of human fluctuating asymmetry have not always followed best practices. The most serious concerns are insensitive asymmetry indices (correlation coefficient and coefficient of indetermination, inappropriate size scaling, unrecognized mixture distributions, inappropriate corrections for directional asymmetry, failure to use composite indices, and inattention to measurement error. Consequently, it is often difficult (or impossible to compare results across traits, and across studies.

  11. Redox Homeostasis and Cellular Antioxidant Systems: Crucial Players in Cancer Growth and Therapy

    Directory of Open Access Journals (Sweden)

    Barbara Marengo

    2016-01-01

    Full Text Available Reactive oxygen species (ROS and their products are components of cell signaling pathways and play important roles in cellular physiology and pathophysiology. Under physiological conditions, cells control ROS levels by the use of scavenging systems such as superoxide dismutases, peroxiredoxins, and glutathione that balance ROS generation and elimination. Under oxidative stress conditions, excessive ROS can damage cellular proteins, lipids, and DNA, leading to cell damage that may contribute to carcinogenesis. Several studies have shown that cancer cells display an adaptive response to oxidative stress by increasing expression of antioxidant enzymes and molecules. As a double-edged sword, ROS influence signaling pathways determining beneficial or detrimental outcomes in cancer therapy. In this review, we address the role of redox homeostasis in cancer growth and therapy and examine the current literature regarding the redox regulatory systems that become upregulated in cancer and their role in promoting tumor progression and resistance to chemotherapy.

  12. Insights into the redox cycle of human quinone reductase 2.

    Science.gov (United States)

    Reybier, Karine; Perio, Pierre; Ferry, Gilles; Bouajila, Jalloul; Delagrange, Philippe; Boutin, Jean A; Nepveu, Françoise

    2011-10-01

    NRH:quinone oxidoreductase 2 (QR2) is a cytosolic enzyme that catalyzes the reduction of quinones, such as menadione and co-enzymes Q. With the aim of understanding better the mechanisms of action of QR2, we approached this enzyme catalysis via electron paramagnetic resonance (EPR) measurements of the by-products of the QR2 redox cycle. The variation in the production of oxidative species such as H(2)O(2), and subsequent hydroxyl radical generation, was measured during the course of QR2 activity under aerobic conditions and using pure human enzyme. The effects on the activity of the following were compared: (i) synthetic (N-benzyldihydronicotinamide, BNAH) or natural (nicotinamide riboside, NRH) co-substrates; (ii) synthetic (menadione) or natural (co-enzyme Q0, Q2) substrates; (iii) QR2 modulators and inhibitors (melatonin, resveratrol and S29434); (iv) a pro-drug activated via a redox cycle [CB1954, 5-(aziridin-1-yl)-2,4-dinitrobenzamide]. The results were also compared with those obtained with human QR1. The production of hydroxyl radicals is: (i) observed whatever the substrate/co-substrate used; ii) quenched by adding catalase; (iii) not observed with the specific QR2 inhibitor S29434; (iv) observed with the pro-drug CB1954. While QR2 produced free radicals with this pro-drug, QR1 gave no EPR signal showing the strong reducing capacity of QR2. In conclusion, EPR analysis of QR2 enzyme activity through free radical production enables modulators and effective inhibitors to be distinguished. © 2011 Informa UK, Ltd.

  13. Chemistry of the redox sensitive elements. Literature review

    International Nuclear Information System (INIS)

    Suter, D.

    1991-10-01

    As a part of the safety assessment for a nuclear waste repository, the migration of the radioactive elements from the waste matrix to the biosphere has to be modelled. The geosphere is an important barrier and a consideration of the retention of the radioactive isotopes needs knowledge of sorption coefficients and solubilities. Important long-lived isotopes in the high level radioactive waste are the fission products selenium, technetium, palladium and tin, and the actinide neptunium, which are all redox sensitive elements. A transport model using conservative sorption values predicts mainly doses from these five elements. Since the individual oxidation states of the redox sensitive elements have different and largely unknown sorption properties and solubilities, the realistic doses might be far less. The relevant literature about the chemistry of the five elements is summarized and is planned to serve as the basis for an experimental programme. For every element, the literature about the general chemistry, selected sorption studies, geochemistry, and analytical methods is reviewed. It was found that the knowledge about some of these points is very limited. Even the general chemistry of some of the elements in not well known, because they have only limited applications and research concentrates only on certain aspects. Most of the sorption studies in the context of nuclear waste concentrate on a few of the relevant elements and others have been neglected up to now. The simulation of a realistic system in the laboratory poses some problems, which have to be solved as well. The literature about this subject is also critically reviewed. The elements which are most mobile under realistic far-field conditions are identified and it is recommended to concentrate research on these at the beginning. (author) 9 figs., 192 refs

  14. Fluctuations and transport in an inhomogeneous plasma

    International Nuclear Information System (INIS)

    Nevins, W.M.; Chen, L.

    1979-11-01

    A formalism is developed for calculating the equilibrium fluctuation level in an inhomogeneous plasma. This formalism is applied to the collisionless drift wave in a sheared magnetic field. The fluctuation level is found to be anomalously large due to both the presence of weakly damped normal modes and convective amplification. As the magnetic shear is reduced, the steady-state fluctuation spectrum is found to increase both in coherence and in amplitude. The transport associated with this mode is evaluated. The diffusion coefficient is found to scale as D is proportional to B 2 /nT/sup 1/2/

  15. Spin-current noise from fluctuation relations

    Energy Technology Data Exchange (ETDEWEB)

    Lim, Jong Soo [Institut de Fisica Interdisciplinària i Sistemes Complexos IFISC (UIB-CSIC), E-07122 Palma de Mallorca (Spain); Sánchez, David; López, Rosa [Institut de Fisica Interdisciplinària i Sistemes Complexos IFISC (UIB-CSIC), E-07122 Palma de Mallorca, Spain and Departement de Fisica, Universitat de les Illes Balears, E-07122 Palma de Mallorca (Spain)

    2013-12-04

    We present fluctuation relations that connect spin-polarized current and noise in mesoscopic conductors. In linear response, these relations are equivalent to the fluctuation-dissipation theorem that relates equilibrium current-current correlations to the linear conductance. More interestingly, in the weakly nonlinear regime of transport, these relations establish a connection between the leading-order rectification spin conductance, the spin noise susceptibility and the third cumulant of spin current fluctuations at equilibrium. Our results are valid even for systems in the presence of magnetic fields and coupled to ferromagnetic electrodes.

  16. Statistical regimes of random laser fluctuations

    International Nuclear Information System (INIS)

    Lepri, Stefano; Cavalieri, Stefano; Oppo, Gian-Luca; Wiersma, Diederik S.

    2007-01-01

    Statistical fluctuations of the light emitted from amplifying random media are studied theoretically and numerically. The characteristic scales of the diffusive motion of light lead to Gaussian or power-law (Levy) distributed fluctuations depending on external control parameters. In the Levy regime, the output pulse is highly irregular leading to huge deviations from a mean-field description. Monte Carlo simulations of a simplified model which includes the population of the medium demonstrate the two statistical regimes and provide a comparison with dynamical rate equations. Different statistics of the fluctuations helps to explain recent experimental observations reported in the literature

  17. Redox regulation of mammalian sperm capacitation

    Directory of Open Access Journals (Sweden)

    Cristian O′Flaherty

    2015-01-01

    Full Text Available Capacitation is a series of morphological and metabolic changes necessary for the spermatozoon to achieve fertilizing ability. One of the earlier happenings during mammalian sperm capacitation is the production of reactive oxygen species (ROS that will trigger and regulate a series of events including protein phosphorylation, in a time-dependent fashion. The identity of the sperm oxidase responsible for the production of ROS involved in capacitation is still elusive, and several candidates are discussed in this review. Interestingly, ROS-induced ROS formation has been described during human sperm capacitation. Redox signaling during capacitation is associated with changes in thiol groups of proteins located on the plasma membrane and subcellular compartments of the spermatozoon. Both, oxidation of thiols forming disulfide bridges and the increase on thiol content are necessary to regulate different sperm proteins associated with capacitation. Reducing equivalents such as NADH and NADPH are necessary to support capacitation in many species including humans. Lactate dehydrogenase, glucose-6-phospohate dehydrogenase, and isocitrate dehydrogenase are responsible in supplying NAD (P H for sperm capacitation. Peroxiredoxins (PRDXs are newly described enzymes with antioxidant properties that can protect mammalian spermatozoa; however, they are also candidates for assuring the regulation of redox signaling required for sperm capacitation. The dysregulation of PRDXs and of enzymes needed for their reactivation such as thioredoxin/thioredoxin reductase system and glutathione-S-transferases impairs sperm motility, capacitation, and promotes DNA damage in spermatozoa leading to male infertility.

  18. Redox regulation of photosynthetic gene expression.

    Science.gov (United States)

    Queval, Guillaume; Foyer, Christine H

    2012-12-19

    Redox chemistry and redox regulation are central to the operation of photosynthesis and respiration. However, the roles of different oxidants and antioxidants in the regulation of photosynthetic or respiratory gene expression remain poorly understood. Leaf transcriptome profiles of a range of Arabidopsis thaliana genotypes that are deficient in either hydrogen peroxide processing enzymes or in low molecular weight antioxidant were therefore compared to determine how different antioxidant systems that process hydrogen peroxide influence transcripts encoding proteins targeted to the chloroplasts or mitochondria. Less than 10 per cent overlap was observed in the transcriptome patterns of leaves that are deficient in either photorespiratory (catalase (cat)2) or chloroplastic (thylakoid ascorbate peroxidase (tapx)) hydrogen peroxide processing. Transcripts encoding photosystem II (PSII) repair cycle components were lower in glutathione-deficient leaves, as were the thylakoid NAD(P)H (nicotinamide adenine dinucleotide (phosphate)) dehydrogenases (NDH) mRNAs. Some thylakoid NDH mRNAs were also less abundant in tAPX-deficient and ascorbate-deficient leaves. Transcripts encoding the external and internal respiratory NDHs were increased by low glutathione and low ascorbate. Regulation of transcripts encoding specific components of the photosynthetic and respiratory electron transport chains by hydrogen peroxide, ascorbate and glutathione may serve to balance non-cyclic and cyclic electron flow pathways in relation to oxidant production and reductant availability.

  19. Redox regulation of mammalian sperm capacitation

    Science.gov (United States)

    O’Flaherty, Cristian

    2015-01-01

    Capacitation is a series of morphological and metabolic changes necessary for the spermatozoon to achieve fertilizing ability. One of the earlier happenings during mammalian sperm capacitation is the production of reactive oxygen species (ROS) that will trigger and regulate a series of events including protein phosphorylation, in a time-dependent fashion. The identity of the sperm oxidase responsible for the production of ROS involved in capacitation is still elusive, and several candidates are discussed in this review. Interestingly, ROS-induced ROS formation has been described during human sperm capacitation. Redox signaling during capacitation is associated with changes in thiol groups of proteins located on the plasma membrane and subcellular compartments of the spermatozoon. Both, oxidation of thiols forming disulfide bridges and the increase on thiol content are necessary to regulate different sperm proteins associated with capacitation. Reducing equivalents such as NADH and NADPH are necessary to support capacitation in many species including humans. Lactate dehydrogenase, glucose-6-phospohate dehydrogenase, and isocitrate dehydrogenase are responsible in supplying NAD (P) H for sperm capacitation. Peroxiredoxins (PRDXs) are newly described enzymes with antioxidant properties that can protect mammalian spermatozoa; however, they are also candidates for assuring the regulation of redox signaling required for sperm capacitation. The dysregulation of PRDXs and of enzymes needed for their reactivation such as thioredoxin/thioredoxin reductase system and glutathione-S-transferases impairs sperm motility, capacitation, and promotes DNA damage in spermatozoa leading to male infertility. PMID:25926608

  20. Redox characterization of functioning skeletal muscle

    Directory of Open Access Journals (Sweden)

    Li eZuo

    2015-11-01

    Full Text Available Skeletal muscle physiology is influenced by the presence of chemically reactive molecules such as reactive oxygen species (ROS. These molecules regulate multiple redox-sensitive signaling pathways that play a critical role in cellular processes including gene expression and protein modification. While ROS have gained much attention for their harmful effects in muscle fatigue and dysfunction, research has also shown ROS to facilitate muscle adaptation after stressors such as physical exercise. This manuscript aims to provide a comprehensive review of the current understanding of redox signaling in skeletal muscle. ROS-induced oxidative stress and its role in the aging process are discussed. Mitochondria have been shown to generate large amounts of ROS during muscular contractions, and thus are susceptible to oxidative stress. ROS can modify proteins located in the mitochondrial membrane leading to cell death and osmotic swelling. ROS also contribute to the necrosis and inflammation of muscle fibers that is associated with muscular diseases including Duchenne muscular dystrophy (DMD. It is imperative that future research continues to investigate the exact role of ROS in normal skeletal muscle function as well as muscular dysfunction and disease.

  1. Environmental heterogeneity generates fluctuating selection on a secondary sexual trait.

    Science.gov (United States)

    Robinson, Matthew R; Pilkington, Jill G; Clutton-Brock, Tim H; Pemberton, Josephine M; Kruuk, Loeske E B

    2008-05-20

    In any population in which resources are limiting, the allocation of resources toward increased reproductive success may generate costs to survival [1-8]. The relationship between a sexually selected trait and fitness will therefore represent a balance between its relative associations with fecundity versus viability [3, 6, 7]. Because the risk of mortality in a population is likely to be heavily determined by ecological conditions, survival costs may vary as a function of the prevailing environment [7]. As a result, for populations experiencing heterogeneous ecological conditions, there may not be a single optimal level of allocation toward reproduction versus survival [9]. Here, we show that early viability and fecundity selection act in opposing directions on a secondary sexual trait and that their relative magnitude depends upon ecological conditions, generating fluctuating selection. In a wild population of Soay sheep (Ovis aries), phenotypic and genetic associations between male horn growth and lifetime reproductive success were positive under good environmental conditions (because of increased breeding success) and negative under poor environmental conditions (because of reduced survival). In an unpredictable environment, high allocation to early horn growth is a gamble that will only pay off if ensuing conditions are favorable. Such fluctuating selection may play an important role in preventing the erosion of genetic variance in secondary sexual traits.

  2. An alternative preparation method for ion exchanged catalysts: Solid state redox reaction

    DEFF Research Database (Denmark)

    Schneider, E.; Hagen, A.; Grunwaldt, J.-D.

    2004-01-01

    A new method for modifying zeolites with zinc is proposed. The solid state redox reaction between metallic zinc and ZSM-5 zeolites with different Si/Al ratios was investigated by temperature programmed hydrogen evolution (TPHE), X-ray absorption near edge structure (XANES) and diffuse reflectance...... should be located at the same sites as in catalysts prepared by conventional methods. Combination of XANES and catalytic activity point to zinc being mainly present in tetrahedral geometry under reaction conditions....

  3. Plant cytoplasmic GAPDH: redox post-translational modifications and moonlighting properties

    Directory of Open Access Journals (Sweden)

    Mirko eZaffagnini

    2013-11-01

    Full Text Available Glyceraldehyde-3-phosphate dehydrogenase (GAPDH is a ubiquitous enzyme involved in glycolysis and shown, particularly in animal cells, to play additional roles in several unrelated non-metabolic processes such as control of gene expression and apoptosis. This functional versatility is regulated, in part at least, by redox post-translational modifications that alter GAPDH catalytic activity and influence the subcellular localization of the enzyme. In spite of the well established moonlighting (multifunctional properties of animal GAPDH, little is known about non-metabolic roles of GAPDH in plants. Plant cells contain several GAPDH isoforms with different catalytic and regulatory properties, located both in the cytoplasm and in plastids, and participating in glycolysis and the Calvin-Benson cycle. A general feature of all GAPDH proteins is the presence of an acidic catalytic cysteine in the active site that is overly sensitive to oxidative modifications, including glutathionylation and S-nitrosylation. In Arabidopsis, oxidatively-modified cytoplasmic GAPDH has been successfully used as a tool to investigate the role of reduced glutathione, thioredoxins and glutaredoxins in the control of different types of redox post-translational modifications. Oxidative modifications inhibit GAPDH activity, but might enable additional functions in plant cells. Mounting evidence support the concept that plant cytoplasmic GAPDH may fulfill alternative, non-metabolic functions that are triggered by redox post-translational modifications of the protein under stress conditions. The aim of this review is to detail the molecular mechanisms underlying the redox regulation of plant cytoplasmic GAPDH in the light of its crystal structure, and to provide a brief inventory of the well known redox-dependent multi-facetted properties of animal GAPDH, together with the emerging roles of oxidatively-modified GAPDH in stress signaling pathways in plants.

  4. Inflammatory cytokines and plasma redox status responses in hypertensive subjects after heat exposure

    Directory of Open Access Journals (Sweden)

    S.F. Fonseca

    2016-03-01

    Full Text Available Hypertension is characterized by a pro-inflammatory status, including redox imbalance and increased levels of pro-inflammatory cytokines, which may be exacerbated after heat exposure. However, the effects of heat exposure, specifically in individuals with inflammatory chronic diseases such as hypertension, are complex and not well understood. This study compared the effects of heat exposure on plasma cytokine levels and redox status parameters in 8 hypertensive (H and 8 normotensive (N subjects (age: 46.5±1.3 and 45.6±1.4 years old, body mass index: 25.8±0.8 and 25.6±0.6 kg/m2, mean arterial pressure: 98.0±2.8 and 86.0±2.3 mmHg, respectively. They remained at rest in a sitting position for 10 min in a thermoneutral environment (22°C followed by 30 min in a heated environmental chamber (38°C and 60% relative humidity. Blood samples were collected before and after heat exposure. Plasma cytokine levels were measured using sandwich ELISA kits. Plasma redox status was determined by thiobarbituric acid reactive substances (TBARS levels and ferric reducing ability of plasma (FRAP. Hypertensive subjects showed higher plasma levels of IL-10 at baseline (P<0.05, although levels of this cytokine were similar between groups after heat exposure. Moreover, after heat exposure, hypertensive individuals showed higher plasma levels of soluble TNF receptor (sTNFR1 and lower TBARS (P<0.01 and FRAP (P<0.05 levels. Controlled hypertensive subjects, who use angiotensin-converting-enzyme inhibitor (ACE inhibitors, present an anti-inflammatory status and balanced redox status. Nevertheless, exposure to a heat stress condition seems to cause an imbalance in the redox status and an unregulated inflammatory response.

  5. Plant cytoplasmic GAPDH: redox post-translational modifications and moonlighting properties

    Science.gov (United States)

    Zaffagnini, Mirko; Fermani, Simona; Costa, Alex; Lemaire, Stéphane D.; Trost, Paolo

    2013-01-01

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis and shown, particularly in animal cells, to play additional roles in several unrelated non-metabolic processes such as control of gene expression and apoptosis. This functional versatility is regulated, in part at least, by redox post-translational modifications that alter GAPDH catalytic activity and influence the subcellular localization of the enzyme. In spite of the well established moonlighting (multifunctional) properties of animal GAPDH, little is known about non-metabolic roles of GAPDH in plants. Plant cells contain several GAPDH isoforms with different catalytic and regulatory properties, located both in the cytoplasm and in plastids, and participating in glycolysis and the Calvin-Benson cycle. A general feature of all GAPDH proteins is the presence of an acidic catalytic cysteine in the active site that is overly sensitive to oxidative modifications, including glutathionylation and S-nitrosylation. In Arabidopsis, oxidatively modified cytoplasmic GAPDH has been successfully used as a tool to investigate the role of reduced glutathione, thioredoxins and glutaredoxins in the control of different types of redox post-translational modifications. Oxidative modifications inhibit GAPDH activity, but might enable additional functions in plant cells. Mounting evidence support the concept that plant cytoplasmic GAPDH may fulfill alternative, non-metabolic functions that are triggered by redox post-translational modifications of the protein under stress conditions. The aim of this review is to detail the molecular mechanisms underlying the redox regulation of plant cytoplasmic GAPDH in the light of its crystal structure, and to provide a brief inventory of the well known redox-dependent multi-facetted properties of animal GAPDH, together with the emerging roles of oxidatively modified GAPDH in stress signaling pathways in plants. PMID:24282406

  6. Nitric oxide-releasing prodrug triggers cancer cell death through deregulation of cellular redox balance

    Directory of Open Access Journals (Sweden)

    Anna E. Maciag

    2013-01-01

    Full Text Available JS-K is a nitric oxide (NO-releasing prodrug of the O2-arylated diazeniumdiolate family that has demonstrated pronounced cytotoxicity and antitumor properties in a variety of cancer models both in vitro and in vivo. The current study of the metabolic actions of JS-K was undertaken to investigate mechanisms of its cytotoxicity. Consistent with model chemical reactions, the activating step in the metabolism of JS-K in the cell is the dearylation of the diazeniumdiolate by glutathione (GSH via a nucleophilic aromatic substitution reaction. The resulting product (CEP/NO anion spontaneously hydrolyzes, releasing two equivalents of NO. The GSH/GSSG redox couple is considered to be the major redox buffer of the cell, helping maintain a reducing environment under basal conditions. We have quantified the effects of JS-K on cellular GSH content, and show that JS-K markedly depletes GSH, due to JS-K's rapid uptake and cascading release of NO and reactive nitrogen species. The depletion of GSH results in alterations in the redox potential of the cellular environment, initiating MAPK stress signaling pathways, and inducing apoptosis. Microarray analysis confirmed signaling gene changes at the transcriptional level and revealed alteration in the expression of several genes crucial for maintenance of cellular redox homeostasis, as well as cell proliferation and survival, including MYC. Pre-treating cells with the known GSH precursor and nucleophilic reducing agent N-acetylcysteine prevented the signaling events that lead to apoptosis. These data indicate that multiplicative depletion of the reduced glutathione pool and deregulation of intracellular redox balance are important initial steps in the mechanism of JS-K's cytotoxic action.

  7. Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.

    Science.gov (United States)

    Blank, Lars M; Ionidis, Georgios; Ebert, Birgitta E; Bühler, Bruno; Schmid, Andreas

    2008-10-01

    A key limitation of whole-cell redox biocatalysis for the production of valuable, specifically functionalized products is substrate/product toxicity, which can potentially be overcome by using solvent-tolerant micro-organisms. To investigate the inter-relationship of solvent tolerance and energy-dependent biocatalysis, we established a model system for biocatalysis in the presence of toxic low logP(ow) solvents: recombinant solvent-tolerant Pseudomonas putida DOT-T1E catalyzing the stereospecific epoxidation of styrene in an aqueous/octanol two-liquid phase reaction medium. Using (13)C tracer based metabolic flux analysis, we investigated the central carbon and energy metabolism and quantified the NAD(P)H regeneration rate in the presence of toxic solvents and during redox biocatalysis, which both drastically increased the energy demands of solvent-tolerant P. putida. According to the driven by demand concept, the NAD(P)H regeneration rate was increased up to eightfold by two mechanisms: (a) an increase in glucose uptake rate without secretion of metabolic side products, and (b) reduced biomass formation. However, in the presence of octanol, only approximately 1% of the maximally observed NAD(P)H regeneration rate could be exploited for styrene epoxidation, of which the rate was more than threefold lower compared with operation with a non-toxic solvent. This points to a high energy and redox cofactor demand for cell maintenance, which limits redox biocatalysis in the presence of octanol. An estimated upper bound for the NAD(P)H regeneration rate available for biocatalysis suggests that cofactor availability does not limit redox biocatalysis under optimized conditions, for example, in the absence of toxic solvent, and illustrates the high metabolic capacity of solvent-tolerant P. putida. This study shows that solvent-tolerant P. putida have the remarkable ability to compensate for high energy demands by boosting their energy metabolism to levels up to an order of

  8. Redox regulation of ischemic limb neovascularization – What we have learned from animal studies

    Directory of Open Access Journals (Sweden)

    Reiko Matsui

    2017-08-01

    Full Text Available Mouse hindlimb ischemia has been widely used as a model to study peripheral artery disease. Genetic modulation of the enzymatic source of oxidants or components of the antioxidant system reveal that physiological levels of oxidants are essential to promote the process of arteriogenesis and angiogenesis after femoral artery occlusion, although mice with diabetes or atherosclerosis may have higher deleterious levels of oxidants. Therefore, fine control of oxidants is required to stimulate vascularization in the limb muscle. Oxidants transduce cellular signaling through oxidative modifications of redox sensitive cysteine thiols. Of particular importance, the reversible modification with abundant glutathione, called S-glutathionylation (or GSH adducts, is relatively stable and alters protein function including signaling, transcription, and cytoskeletal arrangement. Glutaredoxin-1 (Glrx is an enzyme which catalyzes reversal of GSH adducts, and does not scavenge oxidants itself. Glrx may control redox signaling under fluctuation of oxidants levels. In ischemic muscle increased GSH adducts through Glrx deletion improves in vivo limb revascularization, indicating endogenous Glrx has anti-angiogenic roles. In accordance, Glrx overexpression attenuates VEGF signaling in vitro and ischemic vascularization in vivo. There are several Glrx targets including HIF-1α which may contribute to inhibition of vascularization by reducing GSH adducts. These animal studies provide a caution that excess antioxidants may be counter-productive for treatment of ischemic limbs, and highlights Glrx as a potential therapeutic target to improve ischemic limb vascularization. Keywords: Ischemic limb, Angiogenesis, Oxidants, GSH adducts, Glutaredoxin

  9. A robust and versatile mass spectrometry platform for comprehensive assessment of the thiol redox metabolome

    Directory of Open Access Journals (Sweden)

    T.R. Sutton

    2018-06-01

    Full Text Available Several diseases are associated with perturbations in redox signaling and aberrant hydrogen sulfide metabolism, and numerous analytical methods exist for the measurement of the sulfur-containing species affected. However, uncertainty remains about their concentrations and speciation in cells/biofluids, perhaps in part due to differences in sample processing and detection principles. Using ultrahigh-performance liquid chromatography in combination with electrospray-ionization tandem mass spectrometry we here outline a specific and sensitive platform for the simultaneous measurement of 12 analytes, including total and free thiols, their disulfides and sulfide in complex biological matrices such as blood, saliva and urine. Total assay run time is < 10 min, enabling high-throughput analysis. Enhanced sensitivity and avoidance of artifactual thiol oxidation is achieved by taking advantage of the rapid reaction of sulfhydryl groups with N-ethylmaleimide. We optimized the analytical procedure for detection and separation conditions, linearity and precision including three stable isotope labelled standards. Its versatility for future more comprehensive coverage of the thiol redox metabolome was demonstrated by implementing additional analytes such as methanethiol, N-acetylcysteine, and coenzyme A. Apparent plasma sulfide concentrations were found to vary substantially with sample pretreatment and nature of the alkylating agent. In addition to protein binding in the form of mixed disulfides (S-thiolation a significant fraction of aminothiols and sulfide appears to be also non-covalently associated with proteins. Methodological accuracy was tested by comparing the plasma redox status of 10 healthy human volunteers to a well-established protocol optimized for reduced/oxidized glutathione. In a proof-of-principle study a deeper analysis of the thiol redox metabolome including free reduced/oxidized as well as bound thiols and sulfide was performed

  10. Simulation of Temperature Fluctuations in Stirling Engine Regenerator Matrices

    DEFF Research Database (Denmark)

    Andersen, Stig Kildegaard; Carlsen, Henrik; Thomsen, Per Grove

    2003-01-01

    The objective of this study has been to create a model for studying the effects of fluctuations in regenerator matrix temperatures on Stirling engine performance. A one-dimensional model with axial discretisation of engine components has been formulated using the balance equations for mass, energy...... an idealised model. Steady state periodic solutions to the model are found using a custom built shooting method that finds solutions that simultaneously satisfy cyclic boundary conditions and integral conditions. It has been found that true steady state periodic solutions to the model can be reliably...

  11. Impact of uranium (U) on the cellular glutathione pool and resultant consequences for the redox status of U.

    Science.gov (United States)

    Viehweger, Katrin; Geipel, Gerhard; Bernhard, Gert

    2011-12-01

    Uranium (U) as a redox-active heavy metal can cause various redox imbalances in plant cells. Measurements of the cellular glutathione/glutathione disulfide (GSH/GSSG) by HPLC after cellular U contact revealed an interference with this essential redox couple. The GSH content remained unaffected by 10 μM U whereas the GSSG level immediately increased. In contrast, higher U concentrations (50 μM) drastically raised both forms. Using the Nernst equation, it was possible to calculate the half-cell reduction potential of 2GSH/GSSG. In case of lower U contents the cellular redox environment shifted towards more oxidizing conditions whereas the opposite effect was obtained by higher U contents. This indicates that U contact causes a consumption of reduced redox equivalents. Artificial depletion of GSH by chlorodinitrobenzene and measuring the cellular reducing capacity by tetrazolium salt reduction underlined the strong requirement of reduced redox equivalents. An additional element of cellular U detoxification mechanisms is the complex formation between the heavy metal and carboxylic functionalities of GSH. Because two GSH molecules catalyze electron transfers each with one electron forming a dimer (GSSG) two UO(2) (2+) are reduced to each UO(2) (+) by unbound redox sensitive sulfhydryl moieties. UO(2) (+) subsequently disproportionates to UO(2) (2+) and U(4+). This explains that in vitro experiments revealed a reduction to U(IV) of only around 33% of initial U(VI). Cellular U(IV) was transiently detected with the highest level after 2 h of U contact. Hence, it can be proposed that these reducing processes are an important element of defense reactions induced by this heavy metal.

  12. Hydrologic control on redox and nitrogen dynamics in a peatland soil

    International Nuclear Information System (INIS)

    Rubol, Simonetta; Silver, Whendee L.; Bellin, Alberto

    2012-01-01

    Soils are a dominant source of nitrous oxide (N 2 O), a potent greenhouse gas. However, the complexity of the drivers of N 2 O production and emissions has hindered our ability to predict the magnitude and spatial dynamics of N 2 O fluxes. Soil moisture can be considered a key driver because it influences oxygen (O 2 ) supply, which feeds back on N 2 O sources (nitrification versus denitrification) and sinks (reduction to dinitrogen). Soil water content is directly linked to O 2 and redox potential, which regulate microbial metabolism and chemical transformations in the environment. Despite its importance, only a few laboratory studies have addressed the effects of hydrological transient dynamics on nitrogen (N) cycling in the vadose zone. To further investigate these aspects, we performed a long term experiment in a 1.5 m depth soil column supplemented by chamber experiments. With this experiment, we aimed to investigate how soil moisture dynamics influence redox sensitive N cycling in a peatland soil. As expected, increased soil moisture lowered O 2 concentrations and redox potential in the soil. The decline was more severe for prolonged saturated conditions than for short events and at deep than at the soil surface. Gaseous and dissolved N 2 O, dissolved nitrate (NO 3 − ) and ammonium (NH 4 + ) changed considerably along the soil column profile following trends in soil O 2 and redox potential. Hot spots of N 2 O concentrations corresponded to high variability in soil O 2 in the upper and lower parts of the column. Results from chamber experiments confirmed high NO 3 − reduction potential in soils, particularly from the bottom of the column. Under our experimental conditions, we identified a close coupling of soil O 2 and N 2 O dynamics, both of which lagged behind soil moisture changes. These results highlight the relationship among soil hydrologic properties, redox potential and N cycling, and suggest that models working at a daily scale need to consider

  13. Simulating the influence of groundwater table fluctuation on vapor intrusion

    Science.gov (United States)

    Huo, J.

    2017-12-01

    The migration of volatile chemicals from groundwater to an overlying building is a commonly existing phenomenon around the world. Due to the distinction of hydrologic conditions among vapor intrusion sites, it is necessary to consider the effect of dominant hydrologic factors in order to obtain a precise site evaluation and a health risk assessment during the screening process. This study mainly discusses the impact of groundwater table fluctuation and other hydrological factors including porosity, permeability and soil moisture on the vapor intrusion transport. A two-dimensional model is configured to inject different typical volatile organic contaminants from EPA's Vapor Intrusion Database. Through quantifying the contaminant vapor concentration attenuation factors under the effect of groundwater table fluctuation, this study provides suggestions for indoor air sample and vapor intrusion assessment.

  14. Resonant behavior of a fractional oscillator with fluctuating frequency

    Science.gov (United States)

    Soika, Erkki; Mankin, Romi; Ainsaar, Ain

    2010-01-01

    The long-time behavior of the first moment for the output signal of a fractional oscillator with fluctuating frequency subjected to an external periodic force is considered. Colored fluctuations of the oscillator eigenfrequency are modeled as a dichotomous noise. The viscoelastic type friction kernel with memory is assumed as a power-law function of time. Using the Shapiro-Loginov formula, exact expressions for the response to an external periodic field and for the complex susceptibility are presented. On the basis of the exact formulas it is demonstrated that interplay of colored noise and memory can generate a variety of cooperation effects, such as multiresonances versus the driving frequency and the friction coefficient as well as stochastic resonance versus noise parameters. The necessary and sufficient conditions for the cooperation effects are also discussed. Particularly, two different critical memory exponents have been found, which mark dynamical transitions in the behavior of the system.

  15. Resonant behavior of a fractional oscillator with fluctuating frequency.

    Science.gov (United States)

    Soika, Erkki; Mankin, Romi; Ainsaar, Ain

    2010-01-01

    The long-time behavior of the first moment for the output signal of a fractional oscillator with fluctuating frequency subjected to an external periodic force is considered. Colored fluctuations of the oscillator eigenfrequency are modeled as a dichotomous noise. The viscoelastic type friction kernel with memory is assumed as a power-law function of time. Using the Shapiro-Loginov formula, exact expressions for the response to an external periodic field and for the complex susceptibility are presented. On the basis of the exact formulas it is demonstrated that interplay of colored noise and memory can generate a variety of cooperation effects, such as multiresonances versus the driving frequency and the friction coefficient as well as stochastic resonance versus noise parameters. The necessary and sufficient conditions for the cooperation effects are also discussed. Particularly, two different critical memory exponents have been found, which mark dynamical transitions in the behavior of the system.

  16. Gravity waves from quantum stress tensor fluctuations in inflation

    International Nuclear Information System (INIS)

    Wu, Chun-Hsien; Hsiang, Jen-Tsung; Ford, L. H.; Ng, Kin-Wang

    2011-01-01

    We consider the effects of the quantum stress tensor fluctuations of a conformal field in generating gravity waves in inflationary models. We find a nonscale invariant, non-Gaussian contribution which depends upon the total expansion factor between an initial time and the end of inflation. This spectrum of gravity wave perturbations is an illustration of a negative power spectrum, which is possible in quantum field theory. We discuss possible choices for the initial conditions. If the initial time is taken to be sufficiently early, the fluctuating gravity waves are potentially observable both in the CMB radiation and in gravity wave detectors, and could offer a probe of trans-Planckian physics. The fact that they have not yet been observed might be used to constrain the duration and energy scale of inflation. However, this conclusion is contingent upon including the contribution of modes which were trans-Planckian at the beginning of inflation.

  17. Gravity waves from quantum stress tensor fluctuations in inflation

    Science.gov (United States)

    Wu, Chun-Hsien; Hsiang, Jen-Tsung; Ford, L. H.; Ng, Kin-Wang

    2011-11-01

    We consider the effects of the quantum stress tensor fluctuations of a conformal field in generating gravity waves in inflationary models. We find a nonscale invariant, non-Gaussian contribution which depends upon the total expansion factor between an initial time and the end of inflation. This spectrum of gravity wave perturbations is an illustration of a negative power spectrum, which is possible in quantum field theory. We discuss possible choices for the initial conditions. If the initial time is taken to be sufficiently early, the fluctuating gravity waves are potentially observable both in the CMB radiation and in gravity wave detectors, and could offer a probe of trans-Planckian physics. The fact that they have not yet been observed might be used to constrain the duration and energy scale of inflation. However, this conclusion is contingent upon including the contribution of modes which were trans-Planckian at the beginning of inflation.

  18. Scattering of lower-hybrid waves by density fluctuations

    International Nuclear Information System (INIS)

    Andrews, P.L.; Perkins, F.W.

    1981-07-01

    The investigation of the scattering of lower-hybrid waves by density fluctuations in tokamaks is distinguished by the presence in the wave equation of a large, random, derivative-coupling term. Assuming the fluctuations to be of long wavelength compared to the incident wave the similarity of the wave equation to the Schroedinger equation for a particle in a random magnetic field is used to derive a two-way diffusion equation for the wave energy density. The diffusion constant found disagrees with earlier findings and the source of the discrepancy is pointed out. When the correct boundary conditions are imposed this equation can be solved by separation of variables. However most of the important features of the solution are apparent without detailed algebra

  19. Redox zones of a landfill leachate pollution plume (Vejen, Denmark)

    DEFF Research Database (Denmark)

    Lyngkilde, John; Christensen, Thomas Højlund

    1992-01-01

    , ferrogenic, nitrate-reducing and aerobic environments overa distance of 370 m. This redox zone sequence is consistent with thermodynamical principles and is closely matched by the leachate plume determined by the chloride plume distribution. The redox zone sequence is believed to be key in controlling...

  20. Development of redox stable, multifunctional substrates for anode supported SOFCS

    DEFF Research Database (Denmark)

    Sudireddy, Bhaskar Reddy; Foghmoes, Søren Preben Vagn; Ramos, Tania

    2017-01-01

    upon redox cycling, while other properties such as catalytic activity for methane reforming and/or water gas shift, thermal conductivity in addition to electronic conductivity for current pickup are highly wanted for SOFC applications. In order to combine the advantages of a redox stable anode...

  1. Novikov Engine with Fluctuating Heat Bath Temperature

    Science.gov (United States)

    Schwalbe, Karsten; Hoffmann, Karl Heinz

    2018-04-01

    The Novikov engine is a model for heat engines that takes the irreversible character of heat fluxes into account. Using this model, the maximum power output as well as the corresponding efficiency of the heat engine can be deduced, leading to the well-known Curzon-Ahlborn efficiency. The classical model assumes constant heat bath temperatures, which is not a reasonable assumption in the case of fluctuating heat sources. Therefore, in this article the influence of stochastic fluctuations of the hot heat bath's temperature on the optimal performance measures is investigated. For this purpose, a Novikov engine with fluctuating heat bath temperature is considered. Doing so, a generalization of the Curzon-Ahlborn efficiency is found. The results can help to quantify how the distribution of fluctuating quantities affects the performance measures of power plants.

  2. Synchronous imaging of coherent plasma fluctuations.

    Science.gov (United States)

    Haskey, S R; Thapar, N; Blackwell, B D; Howard, J

    2014-03-01

    A new method for imaging high frequency plasma fluctuations is described. A phase locked loop and field programmable gate array are used to generate gating triggers for an intensified CCD camera. A reference signal from another diagnostic such as a magnetic probe ensures that the triggers are synchronous with the fluctuation being imaged. The synchronous imaging technique allows effective frame rates exceeding millions per second, good signal to noise through the accumulation of multiple exposures per frame, and produces high resolution images without generating excessive quantities of data. The technique can be used to image modes in the MHz range opening up the possibility of spectrally filtered high resolution imaging of MHD instabilities that produce sufficient light fluctuations. Some examples of projection images of plasma fluctuations on the H-1NF heliac obtained using this approach are presented here.

  3. Magnetic fluctuations and heavy electron superconductivity

    International Nuclear Information System (INIS)

    Norman, M.R.

    1988-01-01

    A magnetic fluctuation self-energy based on neutron scattering data is used to calculate mass renormalizations, and superconducting critical temperatures and order parameters, for various heavy electron metals

  4. Temperature fluctuations in the Atlantic Ocean

    International Nuclear Information System (INIS)

    Hjoello, Solfrid Saetre

    2005-01-01

    The article discusses the temperature fluctuations in connection with drought in Africa, the climate in North America, the European heat waves and the frequent tropical hurricanes in the Atlantic Ocean. Problems with climate modelling and some pollution aspects are mentioned

  5. Scalar field fluctuations in the early universe

    International Nuclear Information System (INIS)

    Enqvist, K.; Ng, K.W.; Olive, K.A.

    1988-01-01

    We compute the quantum fluctuations of a non-self-interacting but unstable scalar field of arbitrary mass during the period of inflation. Instead of treating the scalar field in a static De Sitter space, we begin with a scalar field in the Friedmann universe just before the start of inflation, and work out the dynamics of the growing quantum fluctuation of the field after it has entered into the inflationary epoch. We use the physically sensible method of Vilenkin to regularize the theory. We find that in all but two special cases the fluctuations produced are different from those in a static De Sitter space, and the effect of the finite width of the scalar field limits the growth of fluctuations. (orig.)

  6. Electric Current Fluctuations, Entropy and Ionic Conductivity

    OpenAIRE

    Zhang, Yong-Jun

    2016-01-01

    This paper reports a relation between ionic conductivity and electric current fluctuations. The relation was derived using statistical analysis and entropy approach. The relation can be used to calculate ionic conductivity.

  7. Event by Event fluctuations and Inclusive Distribution

    OpenAIRE

    Bialas, A.; Koch, V.

    1999-01-01

    Event-by-event observables are compared with conventional inclusive measurements. We find that moments of event-by-event fluctuations are closely related to inclusive correlation functions. Implications for upcomming heavy ion experiments are discussed.

  8. Characterization of edge plasma fluctuations in ASDEX

    International Nuclear Information System (INIS)

    Qin, J.; Carlson, A.; Endler, M.; Giannone, L.; Niedermeyer, H.; Rudyj, A.; Theimer, G.

    1991-04-01

    Nonlinear dynamical characterizations of the edge plasma fluctuations measured by both H α -light diagnostic and Langmuir probes in ASDEX are presented. The edge plasma fluctuations are stochastic rather than chaotic, they have a higher-dimensional structure in phase space. In time, the edge turbulence is found to have memory properties, the time required to lose the memory is different in the different cases. (orig.)

  9. Fluorescence fluctuation spectroscopy (FFS), part A

    CERN Document Server

    Tetin, Sergey

    2013-01-01

    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers fluorescence fluctuation spectroscopyContains chapte

  10. Population Genetics with Fluctuating Population Sizes

    OpenAIRE

    Chotibut, Thiparat; Nelson, David R.

    2016-01-01

    Standard neutral population genetics theory with a strictly fixed population size has important limitations. An alternative model that allows independently fluctuating population sizes and reproduces the standard neutral evolution is reviewed. We then study a situation such that the competing species are neutral at the equilibrium population size but population size fluctuations nevertheless favor fixation of one species over the other. In this case, a separation of timescales emerges natural...

  11. Inertial picobalance reveals fast mass fluctuations in mammalian cells

    Science.gov (United States)

    Martínez-Martín, David; Fläschner, Gotthold; Gaub, Benjamin; Martin, Sascha; Newton, Richard; Beerli, Corina; Mercer, Jason; Gerber, Christoph; Müller, Daniel J.

    2017-10-01

    The regulation of size, volume and mass in living cells is physiologically important, and dysregulation of these parameters gives rise to many diseases. Cell mass is largely determined by the amount of water, proteins, lipids, carbohydrates and nucleic acids present in a cell, and is tightly linked to metabolism, proliferation and gene expression. Technologies have emerged in recent years that make it possible to track the masses of single suspended cells and adherent cells. However, it has not been possible to track individual adherent cells in physiological conditions at the mass and time resolutions required to observe fast cellular dynamics. Here we introduce a cell balance (a ‘picobalance’), based on an optically excited microresonator, that measures the total mass of single or multiple adherent cells in culture conditions